LOCUS       DAZUCC010000001        65918 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZUCC010000001 DAZUCC010000000
VERSION     DAZUCC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.001"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..460)
                     /gene="folK"
                     /locus_tag="ACHY09_000001"
     CDS             complement(<1..460)
                     /gene="folK"
                     /locus_tag="ACHY09_000001"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFS3883027.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELLFPDGLSLVEALQAKGF"
     gene            complement(457..1854)
                     /gene="pcnB"
                     /locus_tag="ACHY09_000002"
     CDS             complement(457..1854)
                     /gene="pcnB"
                     /locus_tag="ACHY09_000002"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFS3883028.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1914..2810)
                     /gene="gluQRS"
                     /locus_tag="ACHY09_000003"
     CDS             complement(1914..2810)
                     /gene="gluQRS"
                     /locus_tag="ACHY09_000003"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFS3883029.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTLFTDQLRGIIHANEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNTQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2877..3332)
                     /gene="dksA"
                     /locus_tag="ACHY09_000004"
     CDS             complement(2877..3332)
                     /gene="dksA"
                     /locus_tag="ACHY09_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFS3883030.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3510..4214)
                     /gene="sfsA"
                     /locus_tag="ACHY09_000005"
     CDS             complement(3510..4214)
                     /gene="sfsA"
                     /locus_tag="ACHY09_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS3883031.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTSC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4229..4759)
                     /gene="thpR"
                     /locus_tag="ACHY09_000006"
     CDS             complement(4229..4759)
                     /gene="thpR"
                     /locus_tag="ACHY09_000006"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFS3883032.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4833..7262
                     /gene="hrpB"
                     /locus_tag="ACHY09_000007"
     CDS             4833..7262
                     /gene="hrpB"
                     /locus_tag="ACHY09_000007"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFS3883033.1"
                     /translation="MSSLPVAAVLPELLAALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVAHFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDTAQMSWLDQPPTVNLQAAKRL
                     LQMLGALEGERLSAQGQKMAVLGSDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIACRRGQDG
                     RYQLANGMGAMLDADDALSRYEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLFAAKWLPEYDWPAVDDESLLATLETWLLPHMSGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDKPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7356..9887
                     /gene="mrcB"
                     /locus_tag="ACHY09_000008"
     CDS             7356..9887
                     /gene="mrcB"
                     /locus_tag="ACHY09_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFS3883034.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDYDDYDDYEDEEP
                     MPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLP
                     AAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDF
                     PDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRS
                     GFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLF
                     LSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYF
                     GRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDM
                     LSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAE
                     KAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIG
                     SLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVD
                     ALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAF
                     QTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTG
                     RQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMS
                     IYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEM
                     QQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10107..12296
                     /gene="fhuA"
                     /locus_tag="ACHY09_000009"
     CDS             10107..12296
                     /gene="fhuA"
                     /locus_tag="ACHY09_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFS3883035.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIASDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12347..13144
                     /gene="fhuC"
                     /locus_tag="ACHY09_000010"
     CDS             12347..13144
                     /gene="fhuC"
                     /locus_tag="ACHY09_000010"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFS3883036.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13144..14034
                     /gene="fhuD"
                     /locus_tag="ACHY09_000011"
     CDS             13144..14034
                     /gene="fhuD"
                     /locus_tag="ACHY09_000011"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFS3883037.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAIIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMIW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14031..16013
                     /gene="fhuB"
                     /locus_tag="ACHY09_000012"
     CDS             14031..16013
                     /gene="fhuB"
                     /locus_tag="ACHY09_000012"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFS3883038.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAINPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16048..17328)
                     /gene="hemL"
                     /locus_tag="ACHY09_000013"
     CDS             complement(16048..17328)
                     /gene="hemL"
                     /locus_tag="ACHY09_000013"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFS3883039.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPHEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17494..17541
                     /gene="yadX"
                     /locus_tag="ACHY09_000014"
     CDS             17494..17541
                     /gene="yadX"
                     /locus_tag="ACHY09_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFS3883040.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17553..18974
                     /gene="clcA"
                     /locus_tag="ACHY09_000015"
     CDS             17553..18974
                     /gene="clcA"
                     /locus_tag="ACHY09_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFS3883041.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            18998..19063
                     /gene="yadW"
                     /locus_tag="ACHY09_000016"
     CDS             18998..19063
                     /gene="yadW"
                     /locus_tag="ACHY09_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFS3883042.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19056..19400
                     /gene="erpA"
                     /locus_tag="ACHY09_000017"
     CDS             19056..19400
                     /gene="erpA"
                     /locus_tag="ACHY09_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFS3883043.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19447..20070)
                     /gene="yadS"
                     /locus_tag="ACHY09_000018"
     CDS             complement(19447..20070)
                     /gene="yadS"
                     /locus_tag="ACHY09_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFS3883044.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20108..20908)
                     /gene="btuF"
                     /locus_tag="ACHY09_000019"
     CDS             complement(20108..20908)
                     /gene="btuF"
                     /locus_tag="ACHY09_000019"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFS3883045.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLVPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20901..21599)
                     /gene="mtnN"
                     /locus_tag="ACHY09_000020"
     CDS             complement(20901..21599)
                     /gene="mtnN"
                     /locus_tag="ACHY09_000020"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS3883046.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21683..23200
                     /gene="dgt"
                     /locus_tag="ACHY09_000021"
     CDS             21683..23200
                     /gene="dgt"
                     /locus_tag="ACHY09_000021"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFS3883047.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLYSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23330..24754
                     /gene="degP"
                     /locus_tag="ACHY09_000022"
     CDS             23330..24754
                     /gene="degP"
                     /locus_tag="ACHY09_000022"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFS3883048.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24909..26066
                     /gene="cdaR"
                     /locus_tag="ACHY09_000023"
     CDS             24909..26066
                     /gene="cdaR"
                     /locus_tag="ACHY09_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFS3883049.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEQR"
     gene            complement(26181..26567)
                     /gene="yaeH"
                     /locus_tag="ACHY09_000024"
     CDS             complement(26181..26567)
                     /gene="yaeH"
                     /locus_tag="ACHY09_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFS3883050.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGIGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26725..27537)
                     /gene="yaeI"
                     /locus_tag="ACHY09_000025"
     CDS             complement(26725..27537)
                     /gene="yaeI"
                     /locus_tag="ACHY09_000025"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFS3883051.1"
                     /translation="MITRRRFLQAAAATLAAGSSLSYMHYWEPGWLELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGVAQKPDLILLGGDYVLFDMQLNFSAFS
                     DVLSSLAECAPTFACYGNHDRPVGTEKNRLIGEALKSAGITVLFNEAMLITTQKQQFE
                     LVGTGDLWTGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIP
                     LVGEPFAPIEDKRYAAGLNAFGERQIYTTRGVGSLYGLRLNCRPEVTILELV"
     gene            complement(27590..28414)
                     /gene="dapD"
                     /locus_tag="ACHY09_000026"
     CDS             complement(27590..28414)
                     /gene="dapD"
                     /locus_tag="ACHY09_000026"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFS3883052.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28445..31117)
                     /gene="glnD"
                     /locus_tag="ACHY09_000027"
     CDS             complement(28445..31117)
                     /gene="glnD"
                     /locus_tag="ACHY09_000027"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFS3883053.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31179..31973)
                     /gene="map"
                     /locus_tag="ACHY09_000028"
     CDS             complement(31179..31973)
                     /gene="map"
                     /locus_tag="ACHY09_000028"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS3883054.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32341..33066
                     /gene="rpsB"
                     /locus_tag="ACHY09_000029"
     CDS             32341..33066
                     /gene="rpsB"
                     /locus_tag="ACHY09_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS3883055.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33324..34175
                     /gene="tsf"
                     /locus_tag="ACHY09_000030"
     CDS             33324..34175
                     /gene="tsf"
                     /locus_tag="ACHY09_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS3883056.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34322..35047
                     /gene="pyrH"
                     /locus_tag="ACHY09_000031"
     CDS             34322..35047
                     /gene="pyrH"
                     /locus_tag="ACHY09_000031"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS3883057.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35339..35896
                     /gene="frr"
                     /locus_tag="ACHY09_000032"
     CDS             35339..35896
                     /gene="frr"
                     /locus_tag="ACHY09_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS3883058.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35988..37184
                     /gene="ispC"
                     /locus_tag="ACHY09_000033"
     CDS             35988..37184
                     /gene="ispC"
                     /locus_tag="ACHY09_000033"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFS3883059.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37373..38131
                     /gene="ispU"
                     /locus_tag="ACHY09_000034"
                     /gene_synonym="uppS"
     CDS             37373..38131
                     /gene="ispU"
                     /locus_tag="ACHY09_000034"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFS3883060.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38144..39001
                     /gene="cdsA"
                     /locus_tag="ACHY09_000035"
     CDS             38144..39001
                     /gene="cdsA"
                     /locus_tag="ACHY09_000035"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS3883061.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39013..40365
                     /gene="rseP"
                     /locus_tag="ACHY09_000036"
     CDS             39013..40365
                     /gene="rseP"
                     /locus_tag="ACHY09_000036"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFS3883062.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVTSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40395..42827
                     /gene="bamA"
                     /locus_tag="ACHY09_000037"
     CDS             40395..42827
                     /gene="bamA"
                     /locus_tag="ACHY09_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFS3883063.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42949..43434
                     /gene="skp"
                     /locus_tag="ACHY09_000038"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42949..43434
                     /gene="skp"
                     /locus_tag="ACHY09_000038"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFS3883064.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43438..44463
                     /gene="lpxD"
                     /locus_tag="ACHY09_000039"
     CDS             43438..44463
                     /gene="lpxD"
                     /locus_tag="ACHY09_000039"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFS3883065.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44568..45023
                     /gene="fabZ"
                     /locus_tag="ACHY09_000040"
     CDS             44568..45023
                     /gene="fabZ"
                     /locus_tag="ACHY09_000040"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS3883066.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45027..45815
                     /gene="lpxA"
                     /locus_tag="ACHY09_000041"
     CDS             45027..45815
                     /gene="lpxA"
                     /locus_tag="ACHY09_000041"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFS3883067.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45815..46963
                     /gene="lpxB"
                     /locus_tag="ACHY09_000042"
     CDS             45815..46963
                     /gene="lpxB"
                     /locus_tag="ACHY09_000042"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFS3883068.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTTRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46960..47556
                     /gene="rnhB"
                     /locus_tag="ACHY09_000043"
     CDS             46960..47556
                     /gene="rnhB"
                     /locus_tag="ACHY09_000043"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS3883069.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRVLGLAS"
     gene            47593..51075
                     /gene="dnaE"
                     /locus_tag="ACHY09_000044"
     CDS             47593..51075
                     /gene="dnaE"
                     /locus_tag="ACHY09_000044"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS3883070.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51088..52047
                     /gene="accA"
                     /locus_tag="ACHY09_000045"
     CDS             51088..52047
                     /gene="accA"
                     /locus_tag="ACHY09_000045"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS3883071.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52146..54287
                     /gene="ldcC"
                     /locus_tag="ACHY09_000046"
     CDS             52146..54287
                     /gene="ldcC"
                     /locus_tag="ACHY09_000046"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFS3883072.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGERWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54344..54733
                     /gene="yaeR"
                     /locus_tag="ACHY09_000047"
     CDS             54344..54733
                     /gene="yaeR"
                     /locus_tag="ACHY09_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS3883073.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54798..56096
                     /gene="tilS"
                     /locus_tag="ACHY09_000048"
     CDS             54798..56096
                     /gene="tilS"
                     /locus_tag="ACHY09_000048"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS3883074.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRMENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELVQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIIGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56145..56405)
                     /gene="rof"
                     /locus_tag="ACHY09_000049"
     CDS             complement(56145..56405)
                     /gene="rof"
                     /locus_tag="ACHY09_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFS3883075.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGTTRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56392..56592)
                     /gene="yaeP"
                     /locus_tag="ACHY09_000050"
     CDS             complement(56392..56592)
                     /gene="yaeP"
                     /locus_tag="ACHY09_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFS3883076.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56758..57303
                     /gene="yaeQ"
                     /locus_tag="ACHY09_000051"
     CDS             56758..57303
                     /gene="yaeQ"
                     /locus_tag="ACHY09_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFS3883077.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFSNLSIWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57300..57722
                     /gene="arfB"
                     /locus_tag="ACHY09_000052"
     CDS             57300..57722
                     /gene="arfB"
                     /locus_tag="ACHY09_000052"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFS3883078.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57736..58446
                     /gene="nlpE"
                     /locus_tag="ACHY09_000053"
                     /gene_synonym="cutF"
     CDS             57736..58446
                     /gene="nlpE"
                     /locus_tag="ACHY09_000053"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414734.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFS3883079.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58476..59300)
                     /gene="yaeF"
                     /locus_tag="ACHY09_000054"
     CDS             complement(58476..59300)
                     /gene="yaeF"
                     /locus_tag="ACHY09_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFS3883080.1"
                     /translation="MDKPKAHCRLLLPCFLFLSACTVDISQPDSSATAVDVEAKTWVV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQVCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(59353..61071)
                     /gene="proS"
                     /locus_tag="ACHY09_000055"
     CDS             complement(59353..61071)
                     /gene="proS"
                     /locus_tag="ACHY09_000055"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS3883081.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61182..61889)
                     /gene="tsaA"
                     /locus_tag="ACHY09_000056"
     CDS             complement(61182..61889)
                     /gene="tsaA"
                     /locus_tag="ACHY09_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFS3883082.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61886..62290)
                     /gene="rcsF"
                     /locus_tag="ACHY09_000057"
     CDS             complement(61886..62290)
                     /gene="rcsF"
                     /locus_tag="ACHY09_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFS3883083.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAESAKPKVP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62408..63223)
                     /gene="metQ"
                     /locus_tag="ACHY09_000058"
     CDS             complement(62408..63223)
                     /gene="metQ"
                     /locus_tag="ACHY09_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFS3883084.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63263..63916)
                     /gene="metI"
                     /locus_tag="ACHY09_000059"
     CDS             complement(63263..63916)
                     /gene="metI"
                     /locus_tag="ACHY09_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFS3883085.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63909..64940)
                     /gene="metN"
                     /locus_tag="ACHY09_000060"
     CDS             complement(63909..64940)
                     /gene="metN"
                     /locus_tag="ACHY09_000060"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFS3883086.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAITWLQEHH
                     VKVEVLGYV"
     gene            65128..65700
                     /gene="gmhB"
                     /locus_tag="ACHY09_000061"
     CDS             65128..65700
                     /gene="gmhB"
                     /locus_tag="ACHY09_000061"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFS3883087.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15670 a        16317 c        18100 g        15831 t
ORIGIN      
        1 taaagccttt tgcctgtaga gcttcaacta atgataagcc atcaggaaat agcaattccg
       61 gcgcaatttc aaacagtggc cacagcataa agccgcgatt tttcatatcg tagtgcggaa
      121 cggtcagacg ttcggtattt attacttcat taccaaacag catgatgtcg agatccagcg
      181 tgcgcggtcc ccagcgttca gctttacgga cgcgaccttg ctgcaattcg atacgctgtg
      241 tgtgattgag tagctcttca ggggcaagag tggttttcag cgcgacggct gcatttaagt
      301 aatcgggttg atcttgcggc cccagcggtg gggtgcggta aaacgaagaa acagcaagaa
      361 tgcggctttc tggaatatcg cctaatgctt tcagggcagc attgacctgc tccagcggag
      421 aggccagatt gctgcctatg gcaatatacg ccactgtcat gcggtaccct cacgacgcgg
      481 tgcgcgtttg cgtggacgac gagtacggcg acgcggtgac ggttcttcat ccagttcgtt
      541 aagcatccct ttttggtctg gtggcgcgga aacctggaac tcaccccacc atttcaccag
      601 acgttgcagt tcagcgttac gctcaacttc ggctcgtaag gccaacaggt cgtatgccgc
      661 acggaactta ggatgctcca gcagtttcca ggcgcgttta ccctgacgtc gggacatacg
      721 caactgcaac tgccagatat cgcgggttaa cgtcgtcaga cgtttcggga tcgccagtga
      781 acgacaggct tcgtccagca cgtcgttcat cgccagcgcg aaagcgtcgt gataggtcag
      841 gccgctttcc tgggcgatct tctgtgccgt ctccagcagt gggtaccaga acatggcggc
      901 aaacaggaac gccgggttca cgcgcatatc gttatggata cgcgtatcgg tattcttcag
      961 cacctgttca atgatccgct ccatcgggct gtcgccattt tccgtgaagt agcgggtaat
     1021 ggtcgggaac agcggctgga acaggtgata ttcacacagc agcttatagg tttcgtaacc
     1081 gtagcccgct tgcagcagtt tcaaagattc ttcaaacaga cgtgccggtg gaatatcgtt
     1141 cagcagggta gccaggcgag ggatcggttc tgcggtttca ggactgatac gcatacccaa
     1201 tttggcggca aaacgtaccg cgcgcagcat ccgtaccgga tcttcacggt agcgcgtttc
     1261 cgggttacca atcagacgga taacgccgtc cttcagatcc ttcatgccgc caacgtaatc
     1321 acggacggta aaatctgcca cgctgtaata caggctgttg atagtgaaat cgcggcgctg
     1381 ggcgtcttct tcgatggagc cgaaaatgtt gtcgcgcagc aacatgccgt tttgcccgcg
     1441 ttgggaggtc gtgcggtcgc tgacgttacc ttcgtgatgt ccacggaagg tcgcaacttc
     1501 gataatctcc gggccaaaca tcacatgagc cagacggaaa cggcgaccca ccaggcggca
     1561 gttacggaac agtttacgta cctgttcagg cgtggcgtta gtggttacgt caaaatcttt
     1621 cggctttttg ccaagtaaca ggtcgcgcac gccaccgcca accagccagg cttcgtatcc
     1681 cgctttattg agcctgtaca ttaccttcag ggcattttca ctgatatctt tgcgggaaat
     1741 agcatgctgc tcacgcggga tcaccgtcac ctgtggacgg gcgactgcct gttcagcctc
     1801 gctttcctcg cggcttagca ccttgcggca aaaattagcg actcgggtaa aaatagtaca
     1861 cctcggtagt gtcaaacatc attcaggaca aaaaaatagc ggctaatcat agctcagcat
     1921 gacgcatttg agaatgttga atttacaatt gccgactcag gcacggcggt aagccgccag
     1981 tttttgacgg ctgactgaag gatttgctcg acgctgaaat cctgccagtg tgcttctgcc
     2041 tgctgaccca gaaattgaag tgccgcgatt agtaccgggc gcggatcgcc tttcggcaac
     2101 gcaggcgcat gattctgctt ggaaagttta gcgccttgtg tgttaagcgc cagcggtaaa
     2161 tgaatgtaat ctggcacttt ccagccaaaa agctggtaca gcgagatttg ccgcactgtc
     2221 ggttcgatca aatctgcccc gcggacaatt tcgctcacgc cctggaagtg atcatccacc
     2281 accaccgcca ggttataggc aaacaaacca tcacggcgat gaataataaa atcttcccgc
     2341 gccagttttt cgttggcgtg aataatgccg cgcagctgat cagtaaatag cgtgaccgga
     2401 tgctgctggc ggatacgtac tgcggcgttg tctggtccat gatgcaattc ccggcaatga
     2461 ccgtcgtaaa taccgccaat gctttgaata cgcgcgcgcg tgcaggtgca gtaataactt
     2521 agtccttgct catgtaacca ggcgagtgct tcgcgatagg catcgtgacg ttgcgattgc
     2581 cagagaacgt cgccgtccca gtgcagaccg taatgttcca gctggcgcag gatagtctct
     2641 gcggcaccgg gaacttcacg aggcgggtcg atatcttcaa tgcgtaccag ccagcgacct
     2701 tgccgggcgc gagcctgtaa atagctgcca agcgcggcaa tcagagagcc aaaatgaagc
     2761 tcgccggaag gagagggggc aaagcggcca atatagtgtg tgtctgtcat ctcgttgaac
     2821 aaaaaataag gcgggagcat ttcccgcctg tggtaaacgt gatggaacgg ctgtatttag
     2881 ccagccattt gtttttcgcg aatttcagcc agcgttttgc agtcgatgca cagatcggct
     2941 gtcgggcgcg cttccagacg gcgaatacca atttcaacac cgcaggattc gcagtagccg
     3001 aaatcttcgt cttccacttt tttcagcgtc ttctcgatct ttttgatcag cttacgctcg
     3061 cgatcgcggt tacgcagttc gaggctgaac tcttcttcct gggctgcacg gtctaccggg
     3121 tccgggaagt tggctgcttc atcctgcata tgtgtaacgg tgcgatcgac ttcatccctg
     3181 agttgattac gccatgcttc cagaatacga cggaagtgcg ccagctgggc ttcattcata
     3241 tactcttcgc ccggcttctc ctgatatggt tccaccccag cgatggcgag aatactcagg
     3301 gacgatgttt tacggttttg cccttcttgc atgttgcttc tccttaacac gcactatcga
     3361 tccccatgtt cgggggaaaa atgaggccgc tataaatagc agatgctttt ccggatagca
     3421 attatctaaa cgtaacactt gacaactgtg tgaggaaaag cgtatttgcg cacgcgacca
     3481 gaatgtaaat taaccagtta catactttac tacaatgtaa ccggcagtga ttttttaaga
     3541 gccatgcctt cagcagaaag ttccgctttg taagccagaa tttctacccc cctctgttga
     3601 gcttctgaca atagttgcgc gtatttctca tcgatgtggc gcgcgggtga aaaccgtgta
     3661 atggctgaat gcagcacggc gaaaaagata accgcacgct ggccttcagc cgctacgctc
     3721 atcaactccc gaaggtgttt ctgacctcgt tcagtgaccg catcgggaaa atatccctgt
     3781 tcgttctccg ctaacgtaac cgatttcact tcaatatagc agtctggacg cgaatccgcc
     3841 tgcaacataa agtcaatacg gctgcgttcg gcgccgtatt ttacttcgct tttcagcgag
     3901 ctatagcctg acagctctga aattgattca ttaaggatag cctctttcgt caacctgtta
     3961 gcccaaagcg tgttgacgca aataaatgcg ccgctctggc tttgagtcaa ttcccaggtg
     4021 tgtgggtatt tccgtttggt gttgtctgaa gtcgaatacc agacggtatc gccaggcgtt
     4081 gcacaactgg tcatcgcacc cgtattcggg cagtgtagcg ttaattcgcg accatcgggt
     4141 gtgatcacat cggctaaaaa acgtttgtaa cgctgaatta gcgtcgcgcg ctgtagaggg
     4201 ggagaaaatt ccattacgac aatccttgtt attgcgttag cgcccagcgt tttagcggcg
     4261 tgtagcgtgt gcgtccacgg gcaaacgagg aggcgtaaag ggtgaactcc gtcaccgtat
     4321 acgaccagtt aaaacctggc ggcgggattg tcaccgcctc gctggcgtcg cgcaataagg
     4381 taatatgtgg atgaaacgga cgattgcttt gaaaacaacc gctgcgggca gcctgtgaac
     4441 ggagcatatt cgccagctgg attaagccgc gtggtggctg acgcatccct aaccacacca
     4501 cacgcgaacg cagccattga ccggcgtcat caagcgtgag tgtgaaaccc ggttgacgaa
     4561 tccgcccggc taaaagagaa agcgccttct ctttctcggc gctcacttcg cctaaaaatg
     4621 ccagagtcag atgcaaatta tcggcggcga ccggacgtcc cgcgtcaggt gggaagtgtg
     4681 tggcgcgcca gtggataatc tgttcgcgga tttctgcagg taagtcgata gcaaagaaca
     4741 gccgttgcgg ttcagacatg tgaggcactc ggttatgaat taccgcgatg ctacaatgtg
     4801 gcgcgaagaa tgttaaccct ctggagcgtt ttgtgtcgtc gttgcccgtt gctgccgtct
     4861 tacctgagtt acttgctgct ctcgattgtg catcgcaggt gttattaagt gcgccgaccg
     4921 gggccgggaa atcaacctgg ctgccgctgc aactgctggc gcatcccggc attaacggga
     4981 aaattatcct gctggagccg cgtcgtctgg cggcgcgtaa cgttgcgcaa cgactggcgg
     5041 agctgcttaa cgaaaagcca ggcgataccg ttggctaccg gatgcgtgcg caaaactgcg
     5101 tcgggccgaa tacccgcctg gaagtggtta ccgaaggcgt gctgacgcgg atgatccagc
     5161 gtgacccgga actgagcggt gttggattgg tgatccttga tgaatttcat gagcgcagct
     5221 tgcaggcgga tttggcgttg gcgctgttac tcgatgtgca acaaggtctg cgtgatgacc
     5281 ttaaactgct gattatgtcg gctacgctgg acaacgaccg cttgcagcaa atgctgccag
     5341 aagcgcccgt cgtcatctca gaagggcgct cgtttccggt tgaacgccgt tatttgccgc
     5401 tgcccgctca tcagcgtttt gacgaagccg tcgcggttgc caccgccgaa atgctgcgtc
     5461 aggaaagtgg atcattactg ttatttttac ctggcgtcgg agaaattcag cgtgtgcagg
     5521 aacaactggc ttcacgcatc ggcagtgatg tgttgctctg cccgctgtat ggcgcgttgt
     5581 cgctgaacga tcagcgaaaa gcgatcctcc cggcaccgca agggatgcgt aaagtggtgc
     5641 tggcgaccaa tattgctgaa accagtttaa ccatcgaagg tattcgtctg gtggtggatt
     5701 gtgcccagga gcgtgtggcg cattttgatc cgcgtacagg gcttacgcgg ctggttactc
     5761 aacgcgttag tcaggcgtcg atgacgcaac gtgccgggcg tgccgggcgt ctggagccgg
     5821 gtatctgcct gcatttaatc gccaaagaac aagcagaacg cgctgccgcg caaagtgaac
     5881 cggagatctt acaaagcgat ctttccggtt tgctgatgga attactgcaa tggggatgca
     5941 gcgatacggc gcagatgagc tggctggatc aaccgccaac ggtgaatcta caggccgcga
     6001 aacggctgtt acaaatgctg ggggcgctgg agggtgaacg gcttagcgca caagggcaaa
     6061 aaatggcagt gctgggtagc gatccgcgtt tagcggcaat gctggttagc gcgaagaacg
     6121 acgacgaagc tgctaccgcg gcaaaaattg ccgccattct cgaagagccg ccacggatgg
     6181 gcaatagtga cctgggcgtg gcgttttcgc gcaatcaacc cgcctggcag caacgtagtc
     6241 agcaactgtt aaaacgctta aacgtacgcg gcggtgaggc agacagttcg cttatcgcgc
     6301 cgctacttgc cggggcgtat gccgatcgca ttgcttgtcg ccgtgggcaa gatggacgct
     6361 atcaactggc aaacggcatg ggggcgatgc tcgatgccga cgatgcgcta agccgctacg
     6421 aatggttgat cgcaccgtta ttattgcagg gcagcgcctc gccggatgcg cggattttac
     6481 tggcgctacc ggtcgatatc gatgaattag tacaacgctg tccgcagctg gtacagcagt
     6541 cggacaccgt ggaatgggat gacgcgcaag gcacgctgaa agcctggcgt cggctgcaaa
     6601 tcggtcagtt gacggtgaaa gtgcagccac tggcgaaacc ctcggaagac gagttgcatc
     6661 aggcgatgct taacggcatc cgtgataaag gtttaagcgt gctcaactgg acggcggaag
     6721 cggaacagct acgcttgcgt ttgttattcg ccgcaaagtg gttgccggaa tatgactggc
     6781 cagcggttga tgatgagagt ttgttggcga cgctggaaac gtggctactg ccgcatatga
     6841 gcggcgtgca ttcactacgc ggcctgaaat cactcgacat ttatcaggcg ctacgcggat
     6901 tacttgattg gggaatgcag caacgtctgg atagtgaatt gcctgcgcat tacactgtgc
     6961 cgacgggaag ccggatcgcc attcgttatc atgaagataa accgcccgcg ctggcggtga
     7021 gaatgcagga gatgtttggt gaggccacca atccgacgat cgcccagggg cgcgtgccgc
     7081 tggtgctgga gttgctttcc cctgcccaaa ggccattaca aatcacacga gatttgagcg
     7141 ccttctggaa aggagcgtac cgtgaggtgc aaaaagagat gaaagggcgt tatcccaaac
     7201 atgtctggcc ggacgacccg gcaaatactg caccgacgcg acggacgaaa aagtattcgt
     7261 gaagaccaaa gcaaatttga gagatatctt cttctgtctt gcaacagaag aacagaaaat
     7321 cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac cgcgagccaa
     7381 ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt cgtcgttacg
     7441 aagatgacga ttacgacgat tatgatgact atgaggatga agaaccgatg ccgcgcaaag
     7501 gtaagggcaa aggcaaaggg cgtaagcctc gtggcaaacg cggctggcta tggctgctgc
     7561 taaaactggc tatcgttttt gccgtgctga tcgccattta cggcgtttat ctcgatcaaa
     7621 aaattcgcag ccgtattgat ggcaaggtct ggcaattgcc cgcggcagtt tatggccgaa
     7681 tggtcaacct tgagccagac atgaccatca gcaagaacga aatggtgaag ctgctggagg
     7741 cgacccagta tcgtcaggtg tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca
     7801 acagcattga gatgatccgc cgtccgtttg atttcccgga cagtaaagaa gggcaggtgc
     7861 gtgcgcgtct gacctttgat ggcgatcatc tggcgacgat cgtcaatatg gagaacaacc
     7921 gtcagtttgg tttcttccgt cttgatccgc gcctgatcac catgatctcc tcgccaaacg
     7981 gtgagcagcg tttgtttgtg ccgcgcagtg gtttcccgga tttgctggtg gatactttgc
     8041 tggcgacaga agaccgccat ttctacgagc atgatgggat cagtctctac tccatcggac
     8101 gtgcggtgct ggcaaacctg accgccggac gcacggtgca gggtgcgagt acgctgacgc
     8161 aacagctggt gaaaaacctg ttcctctcca gcgagcgctc ttactggcgt aaagcgaacg
     8221 aagcttacat ggcgctgatc atggacgcgc gttacagcaa agaccgtatt cttgagctgt
     8281 atatgaacga ggtgtatctc ggtcagagcg gcgacaacga aatccgcggc ttcccgctgg
     8341 cgagcttgta ttactttggt cgcccagtag aagaattgag cctcgaccag caggcgctgt
     8401 tggtgggtat ggtgaaaggg gcgtccatct acaatccgtg gcgtaacccg aaactggcgt
     8461 tggagcgacg taatctggta ctgcgtctgc tgcaacagca acagattatt gatcaagaac
     8521 tctatgacat gttgagtgca cgtccgctgg gggttcagcc gcgcggtggg gtgatctctc
     8581 cacagccagc ctttatgcaa ctggtgcgtc aggagctgca ggcaaaactg ggcgataagg
     8641 taaaagatct ctccggcgtg aagatcttca ctacctttga ctcggtggcc caggacgcgg
     8701 cagaaaaagc cgccgtggaa ggcattccgg cactgaagaa acagcgtaag ttgagcgatc
     8761 ttgaaaccgc gattgtggtc gtcgaccgct ttagtggtga agttcgtgcg atggttggag
     8821 gttctgagcc gcagtttgcg ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt
     8881 cccttgcaaa accagcgact tatctgacgg ccttaagcca gccgaaaatc tatcgcctga
     8941 acacgtggat tgcggatgcg ccaatcgcgc tacgtcagcc gaatggccag gtctggtcac
     9001 cgcagaacga tgaccgtcgt tatagcgaaa gcggcagagt gatgctggta gatgcgttga
     9061 cccgttcgat gaacgtgccg acggtgaatc tgggcatggc gttggggctg cctgcggtta
     9121 cggagacctg gattaaactg ggtgtgccga aagatcaact gaatccagtt ccagcaatgt
     9181 tgttgggggc gttgaactta acgccaatcg aagtggcgca ggcgttccag accatcgcca
     9241 gcggtggtaa ccgtgctccg ctttctgcac tgcgttcggt aatcgcggaa gatggcaagg
     9301 tgctgtatca gagcttcccg caggcggaac gcgctgttcc ggcgcaggcg gcgtatctga
     9361 cactatggac catgcagcag gtggtacaac gtggtacggg gcgtcagctt ggggcgaaat
     9421 acccgaacct gcatctggca gggaaaacag ggactaccaa caataacgta gatacctggt
     9481 ttgcgggcat tgacggcagt acggtgacca tcacctgggt aggccgtgat aacaaccaac
     9541 cgaccaaatt gtatggagcc agcggggcaa tgtcgattta tcagcgttat ctggctaacc
     9601 agacgccaac accgctgaat cttgttccgc cagaagatat tgcagatatg ggcgtggact
     9661 atgacggcaa ctttgtttgc agcggtggca tgcgtgtctt gccggtctgg accagcgatc
     9721 cgcaatcgct gtgccagcag agtgagatgc agcagcagcc gtcaggcaat ccgtttgatc
     9781 agtcttctca gccgcagcaa cagccgcaac agcaacctgc tcagcaagag cagaaagaca
     9841 gcgacggtgt agccggttgg atcaaggata tgtttggtag taattaacgt ctaagcgtga
     9901 cataccggat ggcgagtagc catccggtaa aataacatcc cacccaaagt attaaccctt
     9961 tcttttcatc tggttgttta ttaacccttc aggaaagctc agattgcgta ccgcttgcga
    10021 acccgccagc gtttcgaata ttatcttatc tttataataa tcattctcgt ttacgttatc
    10081 attcacttta tatcagagat ataccaatgg cgcgttccaa aactgctcag ccaaaacact
    10141 cactgcgtaa aatcgcagtt gtagtagcca cagcggttag cggcatgtct gtttatgcac
    10201 aggcagcggt tgaaccgaaa gaagacacta tcaccgttac cgctgcacct gcgccgcaag
    10261 aaagcgcatg gggaccggct gcaactattg cggcgaagca ctctgctact gcgactaaaa
    10321 cggatacacc aattgaaaaa acgccgcagt ctatttcggt tgtgacaaat gaagagatgc
    10381 agatgcatca atttcagtct gtaaaagaag cattaggtta tacacctggc gttacggtta
    10441 gcagtcgtgg tgcttctaat acatatgatt ttgttattat tcgtggtttc tcatctgttg
    10501 gtctgaatca aaataattac cttgatggac taaaacttca gggcaacttt tataacgatg
    10561 ctgtgattga tccttacatg ctcgagaggg ttgaactgat gcgcgggccg acgtctgttc
    10621 tctatgggaa aagtaatcct ggtgggatta tttctatggt gagtaagcgt ccgacaactg
    10681 aacccctgaa agaaattcag tttaaaatgg ggacggataa tctgtttcag accggattcg
    10741 atttcagtga tgcgctggat gataacggtg agttctccta ccgtttgact ggcctcgcac
    10801 gctcaacaaa cgaacagcag aaaaactctg aatctcagcg ttataccatt gctccatcat
    10861 tctcatggcg tccagacgac aaaaccaatt ttactttcct gtcctatttt cagaacgaac
    10921 cagaaacggg ttattacggt tggttgccga aagaggggac cgttgagcca ttgcctaatg
    10981 gtaagcgtct accgactgac ttcaatgaag gtgcatcgaa taatacatac tcccgtaacc
    11041 agaaaatggt gggatatagt tttgaacatg gttttaatga caccttcacc gtgcgtcaga
    11101 atctgcgttt cagtgaaatg aaaacctcac agaaaagtgt ttatggcaca gggattgcca
    11161 gcgatggtca taccctaaac cgcgggacag tggtggataa tgagcgtctg caaaacttta
    11221 gcgttgatac ccaacttgaa agtaaatttg ctacaggtga agtcgagcat actttgctga
    11281 caggggtaga cttcatgcgt atgcgcaatg atatcaatgc cagctttgga tctgcaccat
    11341 ccatcgatct ttataacaaa tatcatcctg aatactttgc atttggtaac gcagagccat
    11401 accaaatgaa tgaaagcaaa caaacaggta tttatgttca ggatcaggcg gaatggaata
    11461 aatgggtatt cactctgggg ggacgctacg attggtctaa gcaagcgacc actgttcgtg
    11521 aaaactctta tacgccgact gaaggttata ttgagcgtaa tgatcatcag ttcacctggc
    11581 gcggtggtgt aaactactta ttcgataatg gtatttcacc ttactttagt tatagccagt
    11641 cctttgaacc gagtgctttc gatctgtgga gcaacccgcg cgtttcctat aagccatcga
    11701 aaggtgaaca gtatgaagct ggcgtaaaat atgttccgaa tgatatgccg gtcgttgtta
    11761 cgggcgcagt ctatcaattg acgaaaacaa ataacctgac agcagaccca acaaacccgt
    11821 tagcgcaagt cccagcaggt gagattcgcg ctcgtggtgt ggaacttgaa gcaaaagcgg
    11881 cgttaaatgc caatattaac ttgacggctt cttataccta cacagatgcg gaatacacca
    11941 aagacaccaa tctcaaaggt aaaactccag aacaagtacc ggagcatatg gcatctctct
    12001 ggggggatta taccttcaat gaagggccgc tttctggttt aacattggga acaggtggtc
    12061 gttttattgg ttccagctat ggtgatccgg cgaacacttt taaagtgggt agcgcagctg
    12121 taatggatgc tgttgtaaaa tatgatctgg cacgctttgg tatggcggga tccagccttg
    12181 ctgtgaacgt caacaatttg ctcgatcgtg agtatgttgc cagttgcttc cagacctatg
    12241 gctgcttctg gggcgcagaa cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttt
    12301 ttctttgggg cacggatttc cgtgcccgct tcacaagttg gctgttatgc aggaatacac
    12361 gaatcattcc gataccactt ttgcactgcg taatatctcc tttcgtgtgc cagggcgcac
    12421 gcttttgcat ccgctgtcgt taacctttcc tgccgggaaa gtgactggtt tgattggtca
    12481 caacggttct ggtaaatcca ctctgctcaa aatgcttggc cgtcatcagc caccgtcgga
    12541 aggcgaaatt cttcttgatg cccagccgct ggaaagctgg agcagcaaag cgttcgcccg
    12601 caaagtggct tatttgccgc agcagcttcc tccggcagaa gggatgaccg tgcgtgaact
    12661 ggtggcgatt ggtcgttacc cgtggcatgg cgcactgggg cgctttggtg cggcagatcg
    12721 cgaaaaggtc gaagaggcga tttcgctggt tggcttaaaa ccgctggcgc atcggctggt
    12781 cgatagtctc tctggcggcg aacgtcagcg ggcgtggatc gctatgctgg tggcgcagga
    12841 tagccgttgt ctgttgctcg acgaaccgac ctcggcgctg gatatcgccc accaggttga
    12901 tgtgctggcg ctggtgcatc gtttaagtca ggaacgtggt ctgacggtca ttgccgtgct
    12961 gcacgatatt aatatggctg cgcgctactg tgattatctg gtcgccctgc gcggtggtga
    13021 aatgattgct cagggaacgc ctgcggaaat tatgcgcggc gaaaccctcg aaatgattta
    13081 tggcatcccg atgggtattc tgccgcatcc ggcgggtgct gcacctgtga gttttgttta
    13141 ttgatgagcg gcttacctct tatttcgcgc cgccgactgt taacggcgat ggcgctttct
    13201 ccgttgttat ggcagatgaa taccgcccac gcggcgatta ttgatcccaa tcgtattgtg
    13261 gcgctggagt ggttgccggt ggaattactg ctggcgctcg gcatcgtgcc ttacggcgtg
    13321 gcggacacca tcaactatcg cctgtgggtc agcgaaccgc cattgccgga ctcagtgatt
    13381 gacgttggtt tgcgtacaga acccaacctt gaactgctga ccgaaatgaa accgtcgttt
    13441 atgatctggt cggcaggata tggcccttca ccagaaatgc tggcacgtat tgcgccgggg
    13501 cgcggattta acttcagcga cggcaaacag ccgctggcga tggcgcgtaa atcactgacg
    13561 gaaatggcag atttacttaa cctgcaaagc gcagcagaaa cgcatttagc gcaatatgaa
    13621 gactttatcc gcagcatgaa accccgcttt gtgaagcgtg gtgcacgccc gttattgctg
    13681 acgacgctta tcgatccgcg ccatatgttg gtcttcggtc caaacagctt gttccaggaa
    13741 attcttgatg agtacggcat cccaaatgcc tggcaagggg aaaccaactt ctggggcagt
    13801 accgccgtca gtatcgatcg tctggcggcg tataaagacg ttgatgtgct ctgttttgat
    13861 cacgacaaca gcaaagacat ggatgcgcta atggcaaccc cgctgtggca ggccatgccg
    13921 tttgtccgtg caggacgttt tcagcgcgtt ccggcggtct ggttctatgg cgcgacgctt
    13981 tcggcaatgc actttgtgcg tattctggat aacgccatcg gaggtaaagc gtgagtaaac
    14041 gaattgcgct ttttccggcg ttattgctgg cgctgctggt gattgtcgct acggcgctca
    14101 cctggatgaa cttctcgcag gcgttgccgc gcagccagtg ggcgcaggcc gcctggtcgc
    14161 cggatattga cgtcatcgag cagatgattt ttcactacag cttgttgccg cgtctggcga
    14221 tttcgctgct ggtgggcgcg ggcctggggc tggtgggcgt gctgtttcag caagtgctgc
    14281 gtaacccgct ggcggaaccg acgacgttgg gcgttgcaac aggcgcgcaa ctggggatta
    14341 ccgtcactac gctctgggcg atcccaggtg ctatggcgag ccagtttgct gcgctggcag
    14401 gggcttgcgt tgttggctta attgtctttg gcgtcgcgtg ggggaaacgg ctgtcgccgg
    14461 taacgctgat cctcgcgggg ctggtagtga gcctttattg tggggcaatc aatcagttac
    14521 tggttatctt ccatcacgac cagctacaaa gcatgttcct gtggagcacc ggaacgctga
    14581 cgcaaaccga ctggggcggc gttgagcgtt tatggccgca gctgctgggc ggcgtgatgc
    14641 tgacgttatt gctacttcgc ccgttaaccc tgatggggct tgatgatggc gtggcgcgca
    14701 atctcggtct ggccttgtcg ctggcgcgtc tggcggcgct gtcgctggcg atagtcatca
    14761 gtgcgctgct ggtgaatgca gtggggatta tcggctttat cgggttgttc gcgccgctgc
    14821 tggcaaaaat gctgggggcg cggcgtctgc tgccacggtt aatgctggcg tcgctgattg
    14881 gtgcgttaat tctgtggctt tctgatcaaa tcatcctctg gctgactcgc gtgtggatgg
    14941 aagtttccac cggttcggtc actgcgttga tcggtgcgcc gctgctactg tggctgctgc
    15001 cgcgtttacg cagcattagc gcgccggata tgaaggtcaa cgatcgtgtc gcggctgaac
    15061 gccaacatgt gctggcgttt gccctcgcgg gcggcgtgct gctgttgatg gctgtggtag
    15121 tggcgctgtc gtttggtcgt gatgcgcacg gctggacgtg ggcgagcggg gcgttgctcg
    15181 aggatttaat gccctggcgc tggccgcgaa ttatggcggc gctgtttgcg ggcgtcatgc
    15241 tggcggtggc gggctgtatt attcagcggc tgaccggaaa cccgatggca agcccggaag
    15301 tgctggggat tagctccggc gcggcgtttg gcgtggtgtt gatgctgttt ctggtgccgg
    15361 gtaatgcctt tggctggctg ttacctgcag gcagtctcgg cgcggcggtg acgctgttga
    15421 tcattatgat cgccgccgga cgcggtggat tttccccaca ccgcatgtta ctggcgggga
    15481 tggcgttaag taccgcgttc accatgcttt tgatgatgtt gcaggcaagt ggtgacccgc
    15541 gaatggcgca agtgctgacc tggatctccg gttcgaccta caacgcgacc gatgcgcagg
    15601 tctggcgcac gggaattgtg atggtaattt tgctggcgat taacccgctg tgccgccgct
    15661 ggctgaccat tttaccgctg ggcggtgata ccgcacgagc cgtaggaatg gcgctgacgc
    15721 cgacgcgaat tgcgctgctg ctattagcgg cttgcctgac ggcgaccgcg acgatgacta
    15781 ttggaccgtt gagttttgtt ggtttaatgg caccgcatat tgcgcggatg atgggttttc
    15841 gacggacgat gccacacatc gtaatttcgg cgctggtggg tggtttactg ctggtgttcg
    15901 ctgactggtg tgggcggatg gtattgtttc cattccagat cccggcgggg ctgctgtcaa
    15961 cctttatcgg cgcgccatat tttatctatt tgttgagaaa gcagagccgt taatagaatg
    16021 cctcgcccgg tgggcgaggc aaaatcatca caacttcgca aacacccgac gtgcagcatc
    16081 gatggtgtta ttgatatctt ccatgctgtg cgccacggac ataaagcccg cttcaaacgc
    16141 tgacggtgcc agataaacac cttcgtccag catcatatgg aagaaacgct taaagcgttc
    16201 cacgtcacag gccatcacat cctgatagca cgtcacggac tcggcgtcgg taaagaaaat
    16261 accgaacatg ccgccaacgt ggtttaccac cagcggaatt ccggcttctt ctgccgcttc
    16321 cagcagacct tctgccagac gtgttgtcag ctcatccagc gtctcgtgaa cgcccggctg
    16381 tgcgacttca ttcagacagg cgaaaccagc tgccatcgca atcgggttac cggaaagcgt
    16441 acccgcctga tagaccggac cagtcggggc cagcgcatcc attacatcac gacgaccacc
    16501 gaatgcgcct accggcattc caccgccgat gattttgccg aggcaggtta aatctggcac
    16561 tacgccgtaa taatcctgtg cgccagctag cgctacgcgg aaaccggtca tcacttcatc
    16621 gatgatcagc aacgcgccaa attcgtcaca cagcgcacgc agacctggca ggaactctgg
    16681 cagcggtgga acgcagttca tattgcctgc caccggctcg acgataatac aggcaatctc
    16741 atgcgggtac tgttcgaacg cggcgcgtac agaggccaga tcgttataag tacaggttaa
    16801 ggtatgtttg gcgaaatctg ccggaacacc cggcgagttt ggctggccta acgtgagtgc
    16861 gccagaaccg gctttcacca gcaggcagtc agcgtgaccg tggtaacaac cttcaaattt
    16921 aatgattttg tcgcgaccgg taaaaccacg ggccaggcgg attgcgctca tggtcgcttc
    16981 agtgccggag ttcaccatgc gcaccatatc catggtcggg accagttcgg tcaccagttg
    17041 cgccattttc acttccattt cggtcggtgc accaaagctt aaaccacgct cggcggcttc
    17101 aatcacggca ttgcggatcg ccggatggtt atggcccagc accatcggcc cccaggaacc
    17161 gacataatcg atataggctt tgccatcaac atcgtacaga taagcgccgt ccgctttttc
    17221 gataaacagt ggagtgccgc ccacgccagt aaaggcgcga acaggggagt tcacaccgcc
    17281 agggatcagc tcgcgcgctg cgctgtaaag attttcagac ttactcatgg agggttcctg
    17341 attcgtagaa aaagtgaatg gctgctattc tatgttattc ataacaagtt aaatactcgt
    17401 caaacatcag gctgcttgta ctggtgcaat cctgatttct tagagtataa aagttttgtg
    17461 catttgaaac attacgcttt gcaaaggatt ttcatggaac gtgcgagtaa aatgccgtca
    17521 tcttatttgt atgaccaata agtgatcatt ggatgaaaac tgatactccc tctttagaaa
    17581 caccgcaggc cgcgcgcctg cgacgcagac aactgattcg ccaacttctt gagcgcgata
    17641 aaaccccgtt agccattttg tttatggcgg cagtcgtcgg cacgcttgtc gggctggctg
    17701 cggttgcttt tgacaaaggt gtcgcctggt tgcagaacca acgtatgggg gcactggtac
    17761 atactgctga taattatccg ctactgttaa ccgtcgcttt tctctgttcg gcggtgctgg
    17821 cgatgttcgg ctactttttg gtgcgcaaat acgcgccgga agcaggtggt tcggggatcc
    17881 cggaaattga aggggcgctg gaagatcaac gtcccgttcg ctggtggcgt gtgttgccgg
    17941 tgaaattctt tggcgggctg ggaacactcg gcggaggcat ggtgctgggc cgcgaagggc
    18001 caaccgtaca gatcggcggc aatatcggtc gcatggtgct tgatgttttc cgcctgaaag
    18061 gtgatgaagc gcgccatacg ctgctggcaa ccggtgctgc ggcggggctg gctgcggcct
    18121 ttaacgcccc gctggcgggt attttgttta ttatcgaaga gatgcgtccg cagtttcgct
    18181 atacgttaat ttcgattaaa gcggtattta ttggcgtcat tatgtcgacc attatgtacc
    18241 ggatttttaa tcatgaagtt gcgttgattg acgtcggtaa actttctgac gcgccgctta
    18301 atacgctgtg gctttatctg atcctcggta ttatttttgg cattttcggc cctattttta
    18361 ataaatgggt gctggggatg caggatttgc tgcaccgtgt gcacggcggc aatattacca
    18421 aatgggtact gatgggcggt gcaattggcg gtctgtgtgg attgctgggg tttgtggcac
    18481 cagcaacgtc gggcggcggt tttaacctga ttcctatcgc taccgcgggg aatttcagca
    18541 tgggaatgct ggtgtttatc ttcgtcgcgc gggtcattac caccttactc tgcttctctt
    18601 ccggcgcgcc gggcggtatt tttgccccga tgctggcgct gggtactgtg ctgggaaccg
    18661 ctttcggaat ggttgccgtt gagctgtttc cgcaatatca ccttgaggcg gggacatttg
    18721 ctattgccgg aatgggggca ttactggcgg catctattcg cgcgccgtta acggggatca
    18781 ttctggttct ggaaatgacc gataactacc agctcatttt gccaatgatt attaccggtc
    18841 ttggcgcaac actattagcg caatttaccg gcgggaaacc gctatactcg gcgattcttg
    18901 cgcgcacgct ggcgaaacag gaagctgagc aactggcgcg aagcaaggcc gcatcagcca
    18961 gcgagaatac ttgaacgaaa taccagggta ttagataatg gcgattatta ttgggttaga
    19021 atttgcccaa ttgccgatgt cgtttggagc aaaatatgag tgatgacgta gcactgccgc
    19081 tggagtttac cgacgcagca gccaacaaag ttaaaagcct gatcgctgac gaagataacc
    19141 cgaatctgaa attacgcgtg tatattaccg gtggcggttg cagcggcttc cagtatggtt
    19201 tcacctttga tgatcaggtg aacgaaggcg atatgaccat cgaaaaacag ggcgttggcc
    19261 tggtggttga tccgatgagc ctgcaatatc tggtcggcgg ttccgttgac tataccgaag
    19321 gtctggaagg ttctcgtttc atcgtgacca acccgaacgc gaaaagcacc tgcggttgcg
    19381 gttcttcctt tagcatctaa tctgttgttc atgtgccgga taaactatcc ggcacatctt
    19441 ctctcttcaa cgcccattct catccagcgc aaacgtcggt agcttaagat gccaacgaat
    19501 agccgccagc cgaatcaata gcgtcacgac catgcccatc atactggctg tttccagtgg
    19561 tacggaaaat gtgtaataag ccgtagcgtg gacaataccg ccgataatac aggctgttgc
    19621 gtagatttct gtacgtaaaa tcatggggat ctcgcgggca agtacatcac gaatgatccc
    19681 gccgccaacg ccagtaatga cgcccataca aaccgcgatt aacggaccgg cttccgcatt
    19741 aaaggcttta ttcacgccaa tgccgacaaa cactgccaga ccaacggcgt ccagcaccgg
    19801 caacatccat tttggtaagc gtcttggctg gcgcaccagc acgatggtca gcatgctggt
    19861 gaccattgca acgaccagat cggtgggatc tttcacccaa aataccgggc cgtgatccag
    19921 cgccatgtcg cgaattgttc cgccgcctac tgcggtaacc acgcccaata ccagaacacc
    19981 aaaagggtcc atacgcaatt ttccggctaa caaaacgcca gagatagcaa ataccgctgt
    20041 gccgactata tccagccaat agacgagcat tgttcaatcc ccactgagca cctgaaaagg
    20101 tcaggcatta atctacctgt gaaagcgcat tacagagctg ttgtgcagcg aggataatac
    20161 gtgggcttgc acgttcaaac cagtcactcg tgagaggaat aacgggaatt ttgagctgtt
    20221 caccccagta ttgtttgatt ttaggaattt ggtccggtcc gcctgtaatg acaatcgcct
    20281 gtggcgcgcg tgctaacacc tgttcgcggc taacttgcgg ccagggaacc cggctgtctt
    20341 taaagatgtt ttctccgcca caaacttcga gtacctggtt ttgaatcgac tcttttccac
    20401 tagtaaatgg cggattaatg ccgaattgca gaaaaacacg ttttttaggt ttatcagcat
    20461 attgcgcttt caattgcgcg tactggtcca gcagggattg cgcggcttgt tcggccttat
    20521 ccggttgcgg actccagggg accagttgac gtaacgcatt ggcaatttgt tcaatgcttg
    20581 tcgcatcgac ccacatcact tttattccca gagaagccag ctggtcaacc tgccgctcgg
    20641 cattacctcc acgccaggca atcaccagat cgggtttcag cgccacaatg cgttccagat
    20701 tcatcccctg ccaggtggaa acctgctcaa ttttttgcgc ttgtggagga tagtcggaat
    20761 agctgctgac cccaaccggc gtgatcccgg cggcaaaggc aagttcagtg ttggcgggag
    20821 aaagcgtgat gacgcgcggc gcggcgttga gccacagcgg cgcgagaaaa gacagggcga
    20881 ccagcgccct gaacagtgac ttagccatgt gccagtttct gcaccagtga ctcaaccatc
    20941 aggctggact gtttagcggc aacagccagg aactcatcga agctaagatg agactgttga
    21001 tcggccacgt cggaaatggc gcgtacgaca acaaacggga cgttgaaatt gtggcagaca
    21061 tgggcgattg ccgtcgcttc catctctaca gcaatggcct gtgggaagtt gtggcggatt
    21121 ttcgccagac caacagaacc attgatgaaa gcgtcgccgc tgacaatcag gccacgtaca
    21181 gcgtttagat tcagttcggc aatgcaggcc tcagcggcag cgatcagttt atcgtcagct
    21241 ttaaagcctg ccgggcagcc tggtaactgg ccgtattcat aaccaaatgc cgtgacatcc
    21301 gcgtcgtgat aacgcgcttc gtccgagaca acgatatcgc ccactttcag tgttggtgcc
    21361 aggccaccgg cagaaccggt gttaataatc acatctggct tgcagtgttc caacagcaaa
    21421 gtggcaccca gcgccgcagc gactttaccg atgcccgatt tcagaagcgc aacctcggtt
    21481 ccattcagtt ggccggtata gatttcgcaa ccgccgagac tgatagtttg acggttttcg
    21541 attttgtcac gcagtagcgt aacttcttct tccattgcac caatgatgcc gattttcata
    21601 gatttactcg cgataagccc gatttgaagg catagtttac catgcgctta cggggaagcg
    21661 tatttctcac gcgggagagg acatggcaca gattgatttc cgaaaaaaaa taaactggca
    21721 tcgtcgttac cgttcaccgc agggcgttaa aaccgaacat gagatcctac ggatcttcga
    21781 gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt cgtctgcaac aaaaaaccca
    21841 ggtttttcca ctggagcgca atgccgccgt gcgcacgcgt cttacccact cgatggaagt
    21901 ccagcaggtg gggcgctaca tcgccaaaga aattttaagc cgtctgaaag agcttaaatt
    21961 actggaagca tacggcctgg atgaactgac cggccccttt gaaagcattg ttgagatgtc
    22021 atgccttatg cacgatatcg gcaatccgcc gtttggtcat tttggcgaag cggcgataaa
    22081 tgactggttt cgccagcgtt tgcacccgga agatgccgaa agccagcctc tgactgacga
    22141 tcgctgcagc gtggcagcac tacgtttacg ggacggggaa gaaccgctta acgagctgcg
    22201 gcgcaaaata cgtcaggact tatgtcattt tgaggggaat gcacaaggca ttcgtctggt
    22261 gcatacattg atgcggatga atctcacctg ggcacaggtt ggcggtattt taaaatatac
    22321 tcgtccggcg tggtggcgtg gtgaaacgcc tgagacacat cactatttaa tgaaaaagcc
    22381 gggttattat ctttctgagg aagcctatat tgcccggttg cgtaaagaac ttaatttggc
    22441 gctttacagt cgttttccat taacgtggat tatggaagct gccgacgaca tctcctattg
    22501 tgtggcagac cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca
    22561 tcatttgcac gaagcgtggg gccagcatga gaaaggttcg ctctattcgc tggtggttga
    22621 aaatgcctgg gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt
    22681 tatgtattta cgggtaaaca ccctaaataa actggtaccc tacgcggcac aacgatttat
    22741 tgataatctg cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag
    22801 cgaatgcagt gatcttctaa agctatataa aaatgtcgct gtaaaacatg tatttagcca
    22861 tccagatgtc gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat
    22921 ttatcgccct ttattaagcc tgtcgttatc agactttact gaactggtag aaaaagaacg
    22981 ggtgaaacgt ttccctattg aatcgcgctt attcaacaaa ctctcgacgc gccatcggct
    23041 ggcctatgtc gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga
    23101 atattattac cgttgccgcc tgctgcagga ttatatcagc ggtatgaccg acctctatgc
    23161 gtgggatgaa taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga
    23221 acaataaatt tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat
    23281 ctgaagaaca cagcaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac
    23341 cacattagca ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc
    23401 aacggcggct gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat
    23461 gctcgaaaag gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa
    23521 tacgccgcgt atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca
    23581 ggaaggttct ccgttccaga gctctccgtt ctgccagggg ggccagggcg gtaatggtgg
    23641 cggccagcaa cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg
    23701 ctatgtcgtc accaacaacc acgttgttga taacgcgacg gtgattaaag tccagctgag
    23761 cgatggccgt aagttcgacg cgaagatggt tggcaaagat ccgcgctctg atatcgcgct
    23821 gatccagatc cagaacccga aaaatctgac cgcaattaag atggcggatt ctgatgcact
    23881 gcgcgtgggt gattacaccg tagcaattgg taacccgttt ggtctgggcg agacggtaac
    23941 ttccgggatt gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt
    24001 catccagacc gatgcagcga tcaaccgtgg taactccggt ggtgcgttgg ttaacctgaa
    24061 cggcgaactg atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat
    24121 cggttttgct atcccgagta acatggtgaa aaacctgacc tcgcagatgg tggaatacgg
    24181 ccaggtgaaa cgcggtgagc tgggtattat ggggactgag ctgaactccg aactggcgaa
    24241 agcgatgaaa gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaactcctc
    24301 cgctgcaaaa gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccaat
    24361 cagcagcttt gccgccctgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac
    24421 cctgggctta ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag
    24481 ccagaatcag gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa
    24541 caaaggcaaa gatcagggcg tggtagtgaa caacgtgaaa acgggcactc cggctgcgca
    24601 gatcggcctg aaaaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat
    24661 cgctgaactg cgtaaagttc tcgacagcaa accgtctgtg ctggcactga acattcagcg
    24721 cggcgacagc accatctacc tgttaatgca gtaatctccc tcaacccctt cctgaatacg
    24781 ggaaggggtt ctccttacaa tctgtgaact tcgccacaac tccatacatc ttcatcatcc
    24841 tttgggcatt tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg
    24901 gaggacttat ggctggctgg catcttgata ccaaaatggc gcaggatatc gtagcacgta
    24961 ccatgcgcat catcgatacc aatatcaacg taatggatgc ccgcgggcga attatcggca
    25021 gcggcgatcg tgagcgtatt ggtgaattgc acgaaggagc attgctggta ctttcacagg
    25081 gacgagtcgt cgatatcgat gacgcggtgg cacgtcatct gcacggtgtg cggcagggga
    25141 ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac
    25201 cagagaatct gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac
    25261 agtcgcggtt gatgcacctg ttggcgcagg atagccgttt gcgggaagaa ctggtgatga
    25321 acctcattca ggctgaggag aatactcccg cacttactga atgggcgcaa cggctgggga
    25381 tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg
    25441 tggacagcgc aatggcggag ttacagcaac tgcaaaatgc gctgactacg cccgagcgta
    25501 ataatctggt ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact
    25561 cttttgggcg ctgggacgca gaagatcatc gtaagagagt tgaacaactg attacccgca
    25621 tgaaagagta tggccagttg cgttttcgcg tttcgctggg caactatttt accggtcctg
    25681 gcagtattgc ccgatcctat cgtacggcga aaacgacgat ggtagtgggg aaacagcgga
    25741 tgccagaaag tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt
    25801 tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg
    25861 ataataacgg cttgctgcga cgtacgctgg cggcgtggtt tcgccataac gtgcagccgc
    25921 tggcaacgtc aaaggcgctg tttattcatc gtaataccct ggagtatcgg cttaatcgta
    25981 tatcggaact cacaggctta gatctgggga attttgatga caggttattg ttgtatgtgg
    26041 cgttacaact ggatgaacag cgatagggta aaaatagtaa atgccgggaa atatgctcgt
    26101 cttaattcaa gttgcatgta cggcgactcg aattattcag cgtatacttt acccggctgg
    26161 cagaatggca atatgcaaaa ttatttacgt gtcagctttt ctaaatctgc ttctatctcg
    26221 ctgattttat tggtcaccac tgactccagg tgacgcaagt cgtcgagaat tttacgctta
    26281 aggtcaactt cgctacgatc acgctggcag atttgatcaa gctcatcaat gatataacga
    26341 agattagggc tgatttcctg aacttcttta tatccctggc caataccatc ggcgaccacc
    26401 gttttgcgct gacgcggata tttaaacttc acactcttgg cgaaaaactc gcctttatct
    26461 ttctgaaaat agattttcaa aatatcgtta ttggcttcct gccggaggct gtaacgatca
    26521 atttcctcag gattggtaat gcccaaactt ttcagattgt cgtacatagt tgtacctcaa
    26581 aagagtcagt aattgtgctt atcttagcat ttcgcctggc ggtttagctc gacggagatc
    26641 gcaggaagcc ttttcttcca gacttttatc atggcctaaa aatcaaaaaa ttacctgctt
    26701 tattctgata agagaattcg ctttctacac cagttccagg attgtcactt ccgggcggca
    26761 attcagacgc aagccataca aacttccgac accccgagtc gtgtaaattt gtcgctcacc
    26821 aaaggcattt aaaccggcgg cgtagcgttt atcttctatg ggcgcaaaag gctcaccaac
    26881 caacggtatg cgtaactgcc cgccgtgggt gtgaccacag agcatcagat cccacttttc
    26941 attacgcatg acttctttgc tgtcgggatt atgcgccagc accagtcgtg gcagatttgc
    27001 ttcgctggcg ggaggcggtt tgcattgtcc ggtccataaa tcacccgtgc caaccagttc
    27061 aaattgctgc ttttgagtgg tgattagcat ggcttcgtta aataacaccg tgatccccgc
    27121 cgattttaat gcctcgccaa ttaagcgatt tttctctgta ccaacagggc gatcgtggtt
    27181 gccgtaacag gcaaatgtcg gcgcacattc ggcaagggag gagagtacgt cactaaacgc
    27241 cgaaaaattc agctgcatat caaataatac gtaatcgccg cccagcaata tcaaatcggg
    27301 cttttgtgct acgccaagag caattgcgtc agaaatcagg cttaaaggaa caaaacgaga
    27361 gtaatggaga tctgccagaa aaaggatttt gaaaggagca gcgttgtctt taaaaaaggc
    27421 gaggcggtga cggattaatt caagccagcc aggttcccaa taatgcatat agctgaggct
    27481 tgatccggca gctaacgtcg cagcggctgc ctgtaaaaaa cgcctgcgtg tgatcatcat
    27541 ccatccctgc aaaaaatcgg gcagcgtcat gctgcccgtg tactgatttt tagtcgatgg
    27601 tacgcagcag ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga
    27661 taactgcaca ataaaggctg tatttgccat ctttcgacgg caggttgcca gaaacaacca
    27721 ccgaaccagc cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg
    27781 tgctctgacc aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt
    27841 caaccacttc agagcgtgca ccgatgaagc aattatcttc aataatggtc gggttagcct
    27901 gcagcggctc cagcacgccg ccgatgccta caccgccaga cagatggacg tttttaccaa
    27961 tttgtgcaca ggaaccgaca gtggcccagg tatcaaccat agtgccttca tcaacatatg
    28021 cgccgatgtt gacgtaagac ggcatcagca cggtgttacg ggcaataaat gcaccctgac
    28081 gtaccgccgc tggtggcaca acgcggaagc cttctttctg aaaacgcgct tcgtcgtagt
    28141 cggcgaattt catcggcact ttatcgaagt agcggctttc tgccccttcg atcacctgat
    28201 tatcattaat acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact
    28261 gaccgtcaat tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat
    28321 ttaccgcttc gcgggttacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa
    28381 aagcggtttc aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa
    28441 cacatcaccc tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct
    28501 gcaactcgtt attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc
    28561 gctcgccaat ggttgtaatt cgggcgccat gaagcgaaat tcccagatcg gcaaaaattt
    28621 tcccgactcg cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc
    28681 ggtcggtatg ggtcggcaaa aacgtcactt cggtttcaac agtaaaatgg cgcaatttgg
    28741 cgggttggcg acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac
    28801 caaaccgaat aacctcatga cgatctgcgg atagcgggct accatcgggt tccagcacga
    28861 tgaaggtatc catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta
    28921 aattgcggcg gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc
    28981 agataaaaat ctcggtgcct ccacgcgtag cctgcggact aagcaatacc agcggtttgc
    29041 ttaaatcatg ctgtaataaa tggcgcgcat gccaggccag ttgatttggg ctatggcgga
    29101 caaaatagtt agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat
    29161 ccatgcgcag tagtgccagc gcctggagtt ggtgatggcg aacccgttcg cgcatatccg
    29221 gcgtgttttg cattccgcgt cgtagctgct tttcggtggc gaaatagagt tcgcgcaaca
    29281 gactttgctt ccagctattc cacagcgttt cgttggtggc gcatatatcg gccactgtca
    29341 ggcataccag ataacgaagt cggttttctg tttgcacttc ttcggcaaac tgcttgatca
    29401 cttccgggtc ctgaatatcg cggcgttggg cggtcaccga catcagcaag tgctggcgaa
    29461 ccagccacgc caccagctgt gtttcgcgcg agttaagccc gtggagttcg gcaaaatgca
    29521 ccacgtcttg tgcaccgaga atggagtggt cgccgccacg ccctttggcg atatcgtgaa
    29581 acagcgcggc gatgaaaatc agctcagttg acggcaggcg cggccagacg tctacacaca
    29641 acggatggcg ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgaa
    29701 tagtatgttc atccaccgtg taggcgtgga acaggtcaaa ctgcatctgc ccgacgatat
    29761 gcgaccattg gggcatatac gcgccgagca cgctatgacg atgcatcggt aatagtccgc
    29821 gacgcaccgc accagggtga cgcagaatgc tcaaaaacag ttttcgcgct tccggaatat
    29881 tacacagcgg ttgttgcaaa tggcgacggg cgtggcgtaa ctggcgcagc gtggtagagt
    29941 aaatgccggt gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg
    30001 gctggcgcat aaatagtgtt tcatcacgca ggtcgatcag cgttccgcgt agctgaaact
    30061 catcgtcgat tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca
    30121 gttgcagcag catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca
    30181 tcatccgctc gaccggttcg ttaccttcgc cactgtaatt cagacgctgg gcgacgctaa
    30241 gctggcgatc aaacagcagg cgattatcgt aacggctgac gaccagatgc agggcaaagc
    30301 gaatacgcca caatatatgc agacattcgt ttaattccgc tcgctccgct gaggttaaga
    30361 agccaaaccc gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt
    30421 gcagagtgtg aatatcgcgc aagccgccag gactactttt gatgtctggt tcaaggttgt
    30481 agctggtgcc gtggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga
    30541 acttgtcgga aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg
    30601 ctacatcgcc aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata
    30661 acccttccag catgcactct tcaagcgtgc gcacgctatg accgacttcc agctttacat
    30721 cccagagcag cgttaacagc tcgcccactt tttgcgcctg atcatccggg agctttttac
    30781 ggcttaaaat cagtaaatcg atgtcggaaa gtggatgcag ctcgccacga ccataaccac
    30841 cgacggcaac caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt
    30901 gcaggagttg gtcgataaac tcggtgcgtg cctcaatcaa ctgttctgcg gagatcccat
    30961 tgtcaaaggc atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga
    31021 cggttaattc atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtgggga
    31081 gagcggtgtt tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa
    31141 ttatcgcatt aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc
    31201 gccgggatgg tgtcatcctt gcgtagcgtc agaatttcgc agccgttatc agtcaccaca
    31261 atagtatgct catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct
    31321 ttcatggtgc ggatctcttt tttacccgcg ttgaccattg gctcgatggt gaacgtcatc
    31381 ccaggtttca gtacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttcg
    31441 tggaagccgc gaccaatacc gtgtccacaa tattcacgaa cgacggagaa gccttctgct
    31501 tcgacaaatt tctgaattgc cgcaccgatt tcgcgcagat taatgccagg ttttaccatg
    31561 cgtagcgcca ggtacaggct ttcttgcgtg atgcggcaca gacgttcgcc catgatggtc
    31621 ggcttaccga cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg
    31681 acatcaatgt taacgatatc gccatctttc agcagcttgg cgtcgtccgg gataccgtgg
    31741 cacaccactt cattaataga aatgcaaacg gatttcggat agccgtgata gccgaggcag
    31801 gcagaaaccg cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg
    31861 gtgctgacgc ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg
    31921 ccagcgacgc gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct
    31981 gtccatcagc gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc
    32041 cgggcaaatt gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat
    32101 agaaaattca acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat
    32161 ttgtggtata aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc
    32221 gacaatctca ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg
    32281 gggtcggtaa tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc
    32341 atggcaactg tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc
    32401 cgttactgga acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc
    32461 atcaaccttg agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt
    32521 gcttctcgca aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg
    32581 aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg
    32641 ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag
    32701 tctcaggacg gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag
    32761 ctggagaaac tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct
    32821 ctgtttgtaa tcgatgctga ccacgaacac attgctatca aagaagcaaa caacctgggt
    32881 attccggtat ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc
    32941 ccgggtaacg acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc
    33001 gtacgtgaag gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct
    33061 gagtaataag gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc
    33121 gagaattccc gggagcttac atcagtaagt gaccgggatg agcgagcgaa gataacgcat
    33181 ctgcggcgcg aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta
    33241 gggggcctgc atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag
    33301 atcacatctc cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc
    33361 gtgagcgtac tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg
    33421 acatcgagct ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag
    33481 caggcaacgt tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca
    33541 ttctggaagt taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg
    33601 cagacaaagt tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gtactgaaag
    33661 cacagttcga agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc
    33721 gccgcgttgc tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg
    33781 gtgttctggt tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg
    33841 ttgctgcaag caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa
    33901 aagaatacca ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag
    33961 agaaaatggt tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc
    34021 cgttcgttat ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag
    34081 tgactggctt catccgcttc gaagtgggtg aaggcatcga gaaagttgag actgactttg
    34141 cagcagaagt tgctgcgatg tccaagcagt cttaattatc gaaaaggagc cgcctgaggg
    34201 cggcttcttt ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa
    34261 gcgacgtaca atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa
    34321 catggctacc aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc
    34381 tctgcagggc actgaaggct tcggtattga tgcaagcata ctggatcgca tggctcagga
    34441 aatcaaagaa ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct
    34501 gttccgtggc gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg
    34561 gatgctggcg accgtaatga acggcctggc aatgcgtgat gcactgcacc gcgcctatgt
    34621 gaacgctcgt ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc
    34681 agaagctatc agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa
    34741 cccgttcttt accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt
    34801 ggtgctgaaa gcaaccaaag ttgacggcgt gtttactgct gatccggcga aagatccaac
    34861 cgcaaccatg tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat
    34921 ggacctggcg gccttcacgc ttgctcgtga ccataaatta ccgattcgtg ttttcaacat
    34981 gaacaaaccg ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac
    35041 ggaataattc ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt
    35101 aagtctggtt ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac
    35161 cggagcgtac aaaagtacgt gaggatggcg agcactgccc ggggccaaaa tggcaaataa
    35221 aatagcctaa taatccagac gattacccgt aatatgttta atcagggcta tacttagcac
    35281 acttccactg tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt
    35341 gattagcgat atcagaaaag atgctgaagt acgcatggat aaatgcgtag aagcgttcaa
    35401 aacccaaatc agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt
    35461 cgtggaatat tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga
    35521 ttcccgtact ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc
    35581 gattatggcg tccgatcttg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc
    35641 gctgccgccg ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc
    35701 agaacaagcg cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc
    35761 actgttgaaa gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca
    35821 gaaactgact gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga
    35881 actgatgcag ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc
    35941 ggctggcggc gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca
    36001 ccattctggg ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc
    36061 ccgaacactt ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac
    36121 agtgcctgga attctctccc cgctatgctg taatggatga tgaagcgagt gcgaaacttc
    36181 ttaaaacgat gctacagcaa cagggtagcc gcaccgaagt cttaagtggg caacaagccg
    36241 cttgcgatat ggcagcgctt gaggatgttg atcaggtgat ggcagccatt gttggcgctg
    36301 ctgggctgtt acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata
    36361 aagaatcact ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc
    36421 aattgttacc ggtcgatagc gaacataacg ccatttttca gagtttaccg caacctatcc
    36481 agcataatct gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg
    36541 ggtctggtgg ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc
    36601 aagcctgccg tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca
    36661 tgatgaacaa aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc
    36721 agatggaagt gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg
    36781 gcagtgttct ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg
    36841 catggccgaa tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt
    36901 tgacatttgc cgcaccggat tatgatcgtt atccatgcct gaaactggcg atggaggcgt
    36961 tcgaacaagg ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgtagctg
    37021 cttttcttgc gcaacaaatc cgctttacgg atatcgccgc gttgaattta tccgtactgg
    37081 aaaaaatgga tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg
    37141 cgcgtgaagt cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca
    37201 gagagtcgcg ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt
    37261 aagtagttgg ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag
    37321 gcttcagtat tcctgagtac cgtaaaccct gtcagggaat aagaaacgcg tgatgttgtc
    37381 tgctactcaa ccacttagcg aaaaattgcc agcgcatggc tgtcgtcatg ttgcgatcat
    37441 tatggacggc aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa
    37501 agccggggca aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc
    37561 gttaacgctg tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc
    37621 gttaatggaa ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa
    37681 cgtgcgtctg cgtattattg gcgataccag tcgctttaac tcgcgtttgc aagaacgtat
    37741 tcgtaaatct gaagcgctaa cagccgggaa taccggtctg acgctgaata ttgcggcgaa
    37801 ctacggtgga cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca
    37861 aggaaacctg caaccagatc agatagatga agagatgcta aaccagcatg tctgtatgca
    37921 tgaactggcc cctgtagatt tagtaattag gactgggggg gagcatcgca ttagtaactt
    37981 tttgctttgg caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt
    38041 cgatgaacaa gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg
    38101 cggcaccgag cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct
    38161 gatatctgct tttgtgttaa tacccgttgt catcgcggcg ttgtttctgt tgccgccggt
    38221 ggggttcgcc attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct
    38281 tagcggtttt accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt
    38341 ggcgctgatg ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga
    38401 aatctcactt tgggcttcgc tgggttggtg gattgtcgcg ctattgctgg tgctgtttta
    38461 ccctggttcc gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct
    38521 aaccattgtt cccttcttct ggggcatgct ggcgttacgg gcctggcact acgacgagaa
    38581 tcattacagt ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc
    38641 tggcgcatat atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc
    38701 gggtaaaacc tggcaaggct ttataggtgg actcgcgacc gcagcggtaa tctcatgggg
    38761 ttatggcatg tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt
    38821 cgcagcgtta gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg
    38881 aattaaggac agcggtcatt taattccagg acacggtggt attttagatc gtattgatag
    38941 cctgacggct gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta
    39001 acggaaggta atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt
    39061 gtacttatca ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc
    39121 gttgagcgtt tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc
    39181 accgaatatg ttatcgccct gatcccgctg ggcggttatg tcaaaatgct ggatgagcgc
    39241 gcagaaccgg tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa
    39301 cgagcggcga ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac
    39361 tggctggttt ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc
    39421 aattcgatag ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt
    39481 atcgaaacgc ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa
    39541 agcaccacca ttacggtagc gccatttggc agcgaccaac ggcgggatgt aaagctcgat
    39601 ttacgtcact gggcgtttga gcctgataaa gaagatccgg taacttcgct ggggattcgt
    39661 cctcgtgggc cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc
    39721 aaggcaggtt tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag
    39781 tgggtgacct ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc
    39841 gaaaggcagg ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt
    39901 aaagcgattg gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa
    39961 gttgtacgcc agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag
    40021 ctgatgaagc tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac
    40081 aacctcagtg ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggtt
    40141 gtttattacc tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt
    40201 ccgttgcccg tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc
    40261 ggaccggtat ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tttgctggtg
    40321 ctgttaatgg ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag
    40381 aacgcataat aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg
    40441 ccaccgtata cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc
    40501 gtgtcgccgt tggtgcggcc ctcctcagta tgccggtgcg cacaggcgac acggttaatg
    40561 atgaagatat cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc
    40621 gcgtccttcg tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca
    40681 gcattacttt ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg
    40741 cttctggcgt gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag
    40801 gtctggaaga cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga
    40861 ccccgctgcc gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg
    40921 aaatccagca aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc
    40981 atttccaact gcgtgacgaa gtgccgtggt ggaacgtggt aggcgatcgt aaataccaga
    41041 aacagaaact ggcgggcgac cttgaaaccc tgcgcagtta ctatctggat cgcggttatg
    41101 cccgtttcaa catcgactct acccaggtca gtctgacgcc tgataaaaaa ggtatttacg
    41161 tcacggtgaa catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca
    41221 accttgccgg gcactccgct gaaattgagc agctgactaa gatcgagccg ggcgagctgt
    41281 ataacggcac caaagtgacc aaaatggaag atgacattaa aaagcttctt ggtcgctatg
    41341 gttatgccta tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta
    41401 aattacgtgt gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag
    41461 gtaacgatac ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat
    41521 ggctggggag cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg
    41581 aaactgtcga taccgatacc caacgcgttc cgggtagccc ggaccaggtt gatgtagtct
    41641 acaaggtgaa agaacgtaac accggtagct tcaacttcgg gattggttac ggtactgaaa
    41701 gtggcgtgag cttccaggct ggtgtgcaac aggataactg gttaggtacg ggttatgctg
    41761 ttggtatcaa cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc
    41821 cgtacttcac cgtagatggt gtaagcctcg gtggtcgtct cttctataat gacttccagg
    41881 cagatgacgc cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg
    41941 gcttcccgat caacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc
    42001 tgtccaacat gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc
    42061 cgagcacctc tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt
    42121 ggacctataa caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga
    42181 ccggtaaagt gaccattccg ggatcggata acgaatacta caaagtgacg ttagacacgg
    42241 cgacttatgt gccgattgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg
    42301 gttatggtga tggtttaggc ggcaaagaga tgccgttcta tgagaacttc tatgccggtg
    42361 gttccagcac cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc
    42421 cgcatcaggc cagtaattat gatccggact atgattacga atgtgcgact caggacggcg
    42481 cgaaagatct gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc
    42541 tcgagttcat caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt
    42601 ccttcttctg ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg
    42661 gatatccgga ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat
    42721 ggatgtcccc attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg
    42781 gagacaaggc agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa
    42841 aggaatgtag tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac
    42901 gcaaagaact gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg
    42961 ttattagctg caggtctcgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt
    43021 gcaatcgtca atatgggcag cctgttccag caggtagcgc agaaaaccgg agtttctaac
    43081 acgctggaaa atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg
    43141 caggctaaaa tgaaaaagct gcagtccatg aaagcgggca gcgatcgcac caaactggaa
    43201 aaagacgtga tggcccagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat
    43261 cgcgcacgtc gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg
    43321 aaatccgttg ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac
    43381 aacagcagcg atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg
    43441 ccttcaattc gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc
    43501 gatatcgtca tcaccggcgt tgcgtccatg caatctgcac aaacaggtca catcacgttc
    43561 atggttaacc caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg
    43621 acccaggacg atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg
    43681 acttacgcgc gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca
    43741 cccagtgcgg tgattgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac
    43801 gcggtgattg agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc
    43861 gtaggtaaaa acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac
    43921 catgagatcc agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac
    43981 ggctttggtt atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta
    44041 attattggcg atcgcgtgga gatcggtgcc tgcactacca ttgatcgtgg cgcgctggat
    44101 gacactgtta ttggcaatgg tgtgatcatt gataaccagt gccagattgc acataacgtc
    44161 gtgattggcg acaatacggc agttgccggt ggcgtcatta tggcgggcag cctgaaaatt
    44221 ggtcgttact gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac
    44281 aaagtgacgg ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat
    44341 tcctcaggca ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg
    44401 aacattgatg acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac
    44461 taacgttcca tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca
    44521 ggccgtgtta ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca
    44581 ctcatactct gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac
    44641 tggtggatcg cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct
    44701 ctgtcaatga gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc
    44761 tgattctgga agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac
    44821 tggaaccggg tgagctgtac tacttcgccg gtattgacga agcgcgcttt aagcgcccgg
    44881 tcgtgcctgg cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga
    44941 cccgttttaa aggggttgct ctggtcgacg gtaaagtagt ttgcgaagca acgatgatgt
    45001 gtgctcgtag ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg
    45061 ccattgtgga agagggcgcg tcgattggcg cgaacgctca cattggtcct ttttgtatcg
    45121 ttggacccca tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg
    45181 gtcatactaa aattggccgc gacaatgaga tttatcagtt cgcctccatc ggcgaagtca
    45241 accaggatct taaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca
    45301 ttcgcgaaag cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg
    45361 gcagcgacaa cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc
    45421 gctgtattct cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga
    45481 tcatcggcgg catgaccgca gtccatcagt tctgcatcat tggtgcgcac gtgatggttg
    45541 gcggctgctc cggtgtggcg caggacgtcc ctccttatgt cattgcgcag ggtaaccacg
    45601 caacgccgtt cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga
    45661 ttaccgctat ccgcaatgcg tataagctga tttatcgtag cggtaaaacg ctcgatgaag
    45721 tgaaaccgga aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt
    45781 tctttgcacg ctcaacgcgc ggtctgattc gttaatgact gaacagcgtc cattaacgat
    45841 tgccctggtc gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct
    45901 gaaagaacgt gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga
    45961 aggctgcgaa gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct
    46021 cggtcgtctg cgtcgcttac tgcatattcg tgccgatctg acaacgcgtt ttggcgaact
    46081 gaagccagat gtttttgtcg gtattgatgc gcctgacttc aatattaccc ttgaaggtaa
    46141 ccttaaaaag cagggtatca aaaccattca ttatgtcagt ccgtccgtct gggcgtggcg
    46201 acagaaacgc gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt
    46261 cgaaaaagcg ttttatgaca aatataacgt gccgtgccgt tttatcggtc acaccatggc
    46321 tgatgccatg ccgttagatc cagataaaaa cgccgcgcgt gatgtgctgg ggatccctca
    46381 cgatgcccac tgtctggcat tgttgccggg aagccgtggc gcggaagtgg agatgcttag
    46441 cgccgatttc ctgaaaacgg cccagctttt gcgccagacg tatcctgatc tggagatcgt
    46501 ggtgccgctg gtaaatgcca aacgccgtga gcaatttgaa cgcatcaaag ctgaagtcgc
    46561 gccagacctt tcggttcata tgctggatgg aatgggccgt gaggcgatgg tcgccagcga
    46621 tgcggcgcta ttggcgtcgg gtacggctgc actggagtgc atgctggcga aatgcccgat
    46681 ggtggtgggg tatcgcatga agcctttcac cttctggttg gcgaagcggc tggtgaaaac
    46741 cgattatgtc tcgctgccaa acctgctggc gggcagagag ttagttaaag aattattgca
    46801 ggaagagtgt gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa
    46861 aaccagccac gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc
    46921 cgatgagcag gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg
    46981 cacacgcacc tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc
    47041 gtcaccgctg cggtgatcct cgacccggcg cgcccgattg ccgggctgaa tgattccaaa
    47101 aagctgagcg aaaaacgccg tctggcgctc tgcgaagaga tcaaagagaa agcgttgagc
    47161 tggagtctgg gccgagcgga acctcacgaa atcgacgaac tgaatatttt acacgcgacc
    47221 atgctggcga tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt
    47281 gatggtaacc gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggcgatagc
    47341 cgcgtaccgg aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg
    47401 gcggcgctgg atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc
    47461 gcttttcatc tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt
    47521 gggcctgtca aacgcgtact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac
    47581 cggaatctga agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg
    47641 atgatcgatg gtctggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg
    47701 ccagcactgg cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg
    47761 ggacatggcg cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg
    47821 ctgggtgatg agttaaccca cctgacggtg ctggcggcga acaataccgg ctatcagaat
    47881 ctgacgttgc tgatctcaaa agcgtatcag cgcgggtacg gtgcagctgg gccgatcatc
    47941 gatcgcgact ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg
    48001 ggcgacgtcg gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg
    48061 ttttatgaag aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg
    48121 gacgaagaaa gctatctgca cgctgcggtg gaactggcgg aagcacgcgg tttgcccgtt
    48181 gtggcgacca acgacgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc
    48241 gtcgcgatcc acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg
    48301 cagcaatata tgcgtagcga agaggagatg tgcgagcttt ttgccgacat tcccgaagcc
    48361 cttgccaaca ccgttgagat cgccaaacgt tgtaacgtaa ccgtgcgtct tggtgaatac
    48421 ttcctgccgc agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca
    48481 aaagaggggc tggaagagcg tctggccttt ttattccctg acgaggaaga acgtgttaag
    48541 cgccgcccgg aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc
    48601 ttcccgggtt acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta
    48661 ccagtagggc caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc
    48721 accgacctcg atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt
    48781 gtctccatgc ctgacttcga cgttgacttc tgtatggaga aacgcgacca ggttatcgag
    48841 cacgtagcgg acatgtacgg tcgtgatgcg gtatcgcaga tcatcacctt cggtacgatg
    48901 gcggcgaaag cggtgatccg cgacgtaggt cgcgtgctgg ggcatccgta cggctttgtc
    48961 gatcgtatct cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa
    49021 gccgagccgc agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac
    49081 atggcgcgca aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt
    49141 atcgcgccga ccaaaattac cgattttgcg ccgctttact gcgatgaaga gggcaaacat
    49201 ccggtcaccc agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc
    49261 cttggtttgc gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg
    49321 gcgaagaatg gcgagccgcc gctggatatc gccgcgatcc cgctggatga taagaaaagc
    49381 ttcgacatgc tgcaacgctc ggaaaccact gcggtattcc agcttgaatc gcgcggcatg
    49441 aaggacctga tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca
    49501 ctgttccgcc ccggtccgtt gcaatcaggg atggtggata actttatcga ccgtaaacat
    49561 ggtcgcgaag agatctccta tccggacgtg cagtggcagc atgaaagcct gaaaccggta
    49621 ctggagccaa cctacggcat catcctgtat caggaacagg tcatgcagat tgcccaggtg
    49681 ctttctggtt ataccctcgg tggcgcagat atgctgcgtc gtgcgatggg taagaaaaag
    49741 ccggaagaga tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc
    49801 aacgccgaac tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt
    49861 aacaaatcgc actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg
    49921 cactatccgg cagagtttat ggcggcggta atgaccgccg atatggacaa taccgagaag
    49981 gtggtgggcc tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata
    50041 aactccggtc tttaccattt ccacgtcaac gacgacggcg aaatcgtgta tggtattggc
    50101 gcgatcaaag gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc
    50161 ggctacttcc gcgaactgtt tgatctctgc gcccgaaccg acaccaaaaa gttaaaccgg
    50221 cgggtgctgg aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgtgcg
    50281 gcgctgatga actcgctggg cgatgcgtta aaagcggcag atcaacacgc gaaagcggaa
    50341 gctatcggtc aggccgatat gttcggcgtg ctggccgaag aaccggaaca aattgaacaa
    50401 tcctacgcca gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg
    50461 ttaggtctgt acctgacggg acaccctatc aaccagtatc taaaagagat tgagcgttat
    50521 gtcggaggcg taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct
    50581 gcggggctcg ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc
    50641 tgcacgctgg atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat
    50701 aaataccagc aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt
    50761 gatgacttca gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc
    50821 cgggaaaaat atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag
    50881 cttttaaacc gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat
    50941 ctctactatc agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc
    51001 tctccgagcg atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa
    51061 ctggagtttg actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg
    51121 attgcagagc tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa
    51181 ctggatatta acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg
    51241 cgtaaaatct tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccacag
    51301 cgtccttata ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc
    51361 gaccgcgcgt atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt
    51421 ccggtgatga tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac
    51481 tttggtatgc cagcgccaga aggttaccgt aaagcactgc gtctgatgca aatggctgaa
    51541 cgctttaaga tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc
    51601 gcagaagagc gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctc
    51661 ggcgtaccgg tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt
    51721 ggcgtgggcg ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa
    51781 ggttgtgcgt ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg
    51841 ggtatcatcg ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg
    51901 ctgggcggcg ctcatcgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg
    51961 gcggatctgg ccgatctcga cgtgttaagc actgaagatt taaaaaatcg tcgttatcag
    52021 cgcctgatga gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt
    52081 ggcccttttt tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg
    52141 aacacatgaa catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca
    52201 tcaaagaact ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca
    52261 gcgttgattt gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact
    52321 gggatgagta cagtctcgat ttatgtagcg atatcaatca gcttaatgaa tatctcccgc
    52381 tttatgcctt catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg
    52441 cgctctggtt ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc
    52501 agtacaccga cgaatatctt gataacatta caccgccgtt cacgaaagcc ttgtttacct
    52561 acgtcaaaga gcggaagtac accttttgta cgccgggtca tatgggcggc accgcatatc
    52621 aaaaaagccc ggttggctgt ctgttttatg attttttcgg cgggaatact cttaaggctg
    52681 atgtctctat ttcggttacc gagcttggtt cgttgctcga ccacaccggg ccacacctgg
    52741 aagcggaaga gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg
    52801 gaacatcgac atcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt
    52861 taattgaccg caattgtcat aaatcgctgg cgcatctgtt gatgatgaac gatgtagtgc
    52921 cagtctggct gaaaccgacg cgtaatgcgt tggggattct tggtgggatc ccgcgccgtg
    52981 aatttactcg cgacagcatc gaagagaaag tcgctgctac cacgcaagca caatggccgg
    53041 ttcatgcggt gattaccaac tccacctatg atggcttgct ctacaacacc gactggatca
    53101 aacagacgct ggatgtcccg tcgattcact tcgattctgc ctgggtgccg tacacccatt
    53161 ttcatccgat ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagtga
    53221 tcttcgaaac gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcgctgatcc
    53281 acattaaagg cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca
    53341 cctcgcccag ttatcccatt gttgcttcgg ttgagacggc agcggcgatg ctgcgtggta
    53401 atccgggcaa acggctgatt aaccgttcag tagaacgagc gctgcatttt cgcaaagagg
    53461 tccagcggct gcgggaagag tctgacggct ggtttttcga tatctggcaa ccgccgcagg
    53521 tggatgaagc cgaatgctgg cccgttgcgc caggcgaacg atggcacggc tttaacgatg
    53581 cggatgccga tcatatgttt ctcgatccgg ttaaagtcac tattctgaca ccggggatgg
    53641 acgagcaggg caatatgagc gaggagggga tcccggcggc gctggtggca aaattcctcg
    53701 acgaacgtgg gatcgtagta gagaaaaccg gcccgtataa cctgctgttt ctttttagta
    53761 ttggcatcga taaaaccaag gcaatgggat tattgcgtgg gttgacggaa ttcaaacgct
    53821 cttacgatct caacctgcgg atcaaaaata tgctacccga tctctatgct gaagatcccg
    53881 atttctaccg caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta
    53941 aacacgatct ttccggtttg atgttgcggg catttgatac cttgccggag atgatcatga
    54001 cgccacatca ggcatggcag agacagatta aaggcgaagt ggaaaccatt gcgctggaac
    54061 aactggtcgg tagagtatcg gcaaatatga tcctgcctta tccacctggc gtaccgctgc
    54121 tgatgccagg agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc
    54181 tttgttccgt agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg
    54241 acgaagacgg cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg
    54301 cttccgggcg agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca
    54361 ggttcaccat attgcgatta ttgcgacgga ttatgcggtg agcaaagcgt tttactgcga
    54421 tattcttggt ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg
    54481 ggatttggcg cttaatgggc aatatgtgat tgagcttttc tcatttccgt tcccgccgga
    54541 gcgccccagc cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgatat
    54601 cgatgcggca gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga
    54661 tccatacacg caaaaacgct tcacgttctt taacgatccg gacgggctgc cgttggaact
    54721 gtatgagcag taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac
    54781 tgtaaaatcg catcatcatg acactcacgc tcaatagaca acttctctcc tcacgccaga
    54841 ttctggtggc ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt
    54901 ggcggatgga aaatccgggc gtcactctgc gcgctatcca tgtgcatcac ggtttaagtg
    54961 ctaatgccga tgcctgggtt aggcattgcg agaacatctg ccaacagtgg caggtgccgc
    55021 tggtggtcga gcgcgtgcaa cttgcgcaag aaggactggg cattgaggcc caggcgcggc
    55081 aggcacgtta tcaggcattt gcccgcacct tgttacccgg tgaagtgctg gtcaccgcgc
    55141 aacatctcga cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg
    55201 ccgggctttc ggctatgggg gaagtttcgg agtttgccgg aacacggctt atccgcccgt
    55261 tgctcgcccg cacgcgggga gaactggtgc agtgggcgcg tcagtatgat ttacgctgga
    55321 ttgaagacga aagtaatcag gatgatagct acgatcgtaa cttcctgcgc ctgcgcgtgg
    55381 tgccgttatt gcagcagcgt tggccgcatt ttgccgaagc aacggcccgc agcgctgcgc
    55441 tttgcgctga acaagagagc ctgttggatg aactgctggc ggatgatata gcacactgtc
    55501 aaacgtcgca ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgccgcg
    55561 cggcgattat ccgccgctgg ctggcaggga agaatgcacc gatgccttcc cgcgacgcgt
    55621 tggtgaggat ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgcc
    55681 tgggcgcgtt tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcactg
    55741 ggcaaagcga aaccattgtg ccgtggcaga cgtggctaca accgctggaa ttaccggcgg
    55801 gactgggaag tgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag
    55861 cggtcagcgt gcgtttcaaa gcgccaggat tgctgcatat tatcgggcgc aacggcggac
    55921 gtaagctgaa gaaaatctgg caagagctgg gtgtgccgcc gtggctacgt gacaccacgc
    55981 cactgctgtt ttatggcgaa acgttgattg cggcggcagg ggtatttgtg acgcaagaag
    56041 gtgtggctga aggtgagaat ggcgtcagtt ttgtctggca gaaaacgctt agttaagtga
    56101 aagccggata agacgcttca aacgtcgcat ccggcgaaag tcaatcagga ctcgctcacc
    56161 accaccgtac cgatttccgg gtggctaaag ctggtaattt tatccagacg cagctcgcgg
    56221 gttgtaccag cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcgctg
    56281 gccttcgcct gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg
    56341 caggcgagct caagattatc gtaatcatca caattgattg gttgatacgt atcattcatt
    56401 gacataatcg ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc
    56461 agaaagggcg tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc
    56521 cggaacgtat cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca
    56581 atatttttcc acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc
    56641 ctacgaagat aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag
    56701 cgccgcaact gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg
    56761 gcgcttaaag cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag
    56821 tttctcgatg cctctctgac gctggcgcgc cacccttcag aaacccagga gcgcatgatg
    56881 ctgcgcctgc tggcgtggct aaaatatgcc gatgaacgtc tgcaatttac ccgtggtttg
    56941 tgtgccgatg atgagccgga agcgtggctg cgtaacgatc atttgggtat tgatttgtgg
    57001 attgagctgg ggctgccgga tgagcgacgg attaagaaag cctgcaccca ggccgcagaa
    57061 gtggcgctgt ttgcctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc
    57121 aaatgcgcgc agttttccaa tctttccatc tggtatctgg acgatgaaca actggcgaaa
    57181 gtgagcgcct ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggcgtgatc
    57241 tggttatcgg atgataagaa taatctggaa gtgaacttaa ccgcctggca acaaccttca
    57301 tgattgtgat ttcccgacat gttgctatcc ccgatggtga gcttgagatc accgccattc
    57361 gtgcgcaggg cgcgggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt
    57421 ttgacattcg ggcgtccagc ctgccagagt attacaaaga acgtctgctc gccgccagcc
    57481 atcacttgat cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg
    57541 aactgaaccg cgaagcggct ctggcccggc tggtggcagt gattaaagat ttaacaacag
    57601 aacaaaaagc ccgacgaccc acgcgcccca cccgtgcatc gaaagagcgc aggctggcat
    57661 cgaaagcaca aaaatcaagc gtgaaggcga tgcgcggcaa agtgcgcagc ggtcgggaat
    57721 aaaaagaagg aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc
    57781 tttactctga tgggttgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct
    57841 gccgaactga aaccgatgcc gcaaagttgg cgcggcgtgc tgccgtgtgc tgattgcgaa
    57901 ggaatcgaaa cctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat
    57961 ctcggtgctc gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct
    58021 gacaagctag tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat
    58081 gcgctggaga tgctcgatcg tgaaggcaat ccgattgaat cgcagttcaa ctatacgcta
    58141 gaaccggcac aatccagttt acctatgacg ccgatgaccc tgcggggcat gtatttttat
    58201 atggctgatg cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat
    58261 aacgcagagc tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta
    58321 ttgtcagtag aaggtcactt tacgcttgag ggtaatccgg ataccggtgc gccgactaaa
    58381 gtattggcac ccgatacggc gggtaaattt tatccgaacc aggattgcag tagtttgggg
    58441 cagtaattga aagccggagc attctccggc ttttatcact gagtcagccc aacaaagcga
    58501 cccgccttga tataaatccc tggcttcatg tgcccaacat actgtaactg cgtttctggc
    58561 ttaaacgcag agatatcgcc agtacgcata tgcatcagat cggcagggct aatccagcct
    58621 gattgcgcca gcgtaagcgg atgcccggct ttggcaaatg catcagtgac aaattccgaa
    58681 caaaaccacg actttttgtc tccttcgccc acactgctta actgcgcttt cgccaggccg
    58741 ctgacgcact gttgacgaaa atcctcggag aacggattca gtgagcacac ctggcgagtc
    58801 accataaagg gaataaattc gacaatgccg cgatagttat aaccgctatc tttgatttta
    58861 ttagcaaacg cggtgatatc tgtggcttgt tgcggggtaa gatccgggac tcgtaagacg
    58921 aaaagcttat cactgtgctt catcgctttt ttaagggaaa cgatctggac gccagcgcct
    58981 gtcgcttctg caacgttatt atcacccaga aagattgcaa cgtgactcac agaggaagtg
    59041 ctgaagacgc ggattccaaa tgaggttacc ccaaggcttg aggagaacag caaatcgccg
    59101 ggtttgagat caggtgccgt tatttctttt attgattgtt cggtgaaaga gctttgatgc
    59161 tggaatttga ctacccatgt ttttgcctcg acatctaccg ctgtggcaga ggagtctggc
    59221 tggctgatat caacggtaca ggccgaaagg aaaagaaagc aggggagaag caggcggcaa
    59281 tgcgcctttg gtttatccat tttatacaat ccatgtaaaa aagggccctg aaattcagga
    59341 ccctttctgg catcagcctt taatctgttt caccagatat tcgacgatgt caccagtctt
    59401 aattaactgt ttctcgccgt tacgacgata tttatattcg atatcatcgt tgtcgaggtt
    59461 acggtcgccc agcacaatag tgtgcggaat accgatcagt tccatatcag caaacatcac
    59521 gcccggacgc tctttgcggt catccagcag cacttcgata ccttgtgcac gcagttcgct
    59581 gtacagtttc tcagcaagct cttgtacgcg gaaggatttg tgcatgttca tcggcagaat
    59641 cgccacctgg aaaggcgcga tagcgtcagg ccatacgatg ccgcgttcgt catagttctg
    59701 ctcaattgcc gcagccacaa cgcgcgttac cccgataccg tagcaaccca tcgtcaggat
    59761 ttgattacgg ccatcttcac cctgtacgga ggctttcagt gcttcggagt acttggtacc
    59821 cagctggaag atgtgaccaa cttcgatacc acgtttgatc agcagcgtac cctgaccatc
    59881 cgggcttgga tcgccagcca ccacgttgcg gatatcagca atttccgggg tagcgacatc
    59941 gcgatcccag ttgataccga agtagtgttt accatcgatg ttagcaccag cagcgaaatc
    60001 actcatcgcc gcaacggtac ggtcaatcac caccggaatt ggcatgttta ccggacccag
    60061 tgagccaggg ccggctttaa ccacggcacg aatttcttct tcggtcgcga aagtcagcgg
    60121 gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc agctcgtggt caccgcgcac
    60181 cagcagcgca accagcggga agctactgcc ttcaaccgct ttaaccagca gggtcttaac
    60241 cgttttctca atcggcagat tgaactgttc aaccagttcc gcgatggtct tcgcgttcgg
    60301 cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc ggttctttcg gcgcgatagc
    60361 ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg gagaagacca catcgtcttc
    60421 accgctttgc gccagcacct ggaattcgtg agaggcgcta ccgccgatag aaccggtatc
    60481 ggcctgtacg gcgcggaaat ccagccccat gcggctgaag attttgctgt aggccgcata
    60541 cattgcatcg taggtttcct gcagggattc ctgagaggta tggaaagagt aagcatcttt
    60601 catcaggaat tcgcgggaac gcatgacgcc gaaacgcggg cgcacttcat cacggaactt
    60661 agtctggatc tgatagaagt tcagcggcag ctgtttgtaa gagctcagct cgttacgaat
    60721 caggtcggta atcacttctt catgagttgg gccaagtacg aacggacggt caccacggtc
    60781 agcaatacgc agcaattccg ggccatactg ttcccaacga ccactctctt gccacagttc
    60841 agatggctga accaccggca ttaacacctc aatcgcaccg gcgttgttca tctcttcacg
    60901 cacgatgttt tcgacttttt tcagaacgcg cacgccggtc ggcagccagg tgtataaccc
    60961 ggaggccagc ttgcggatca tcccggcgcg cagcatcagc tgatggctga tcacctcggc
    61021 gtcggcaggt gtctccttga gagtggagag caggtattgg ctagtacgca tgttgttacg
    61081 gttccagttg gaaggtagaa caggctcaag gtgagcctgg gacaaaaaaa gtgatttagt
    61141 ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg ttccagcgca
    61201 aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg cagccagacg
    61261 gcataggttt tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc ctgcgctagt
    61321 acttcgcgga taaacagcgt taactgcgga taacgcttct ccagcgtcaa aagctgcttt
    61381 tcgacctctg cggtaaaact caccgccatc tctgcagctg gcgcactttg cgcatagctg
    61441 gcactggcat ttggaagcga ttcggcaaag gggagatacg gtttgatatc cactaccggc
    61501 gtaccatcga ccagatccag actgccgagc ttcagaatca cgctgtcttt atggcaaaca
    61561 acctctttca gctctaccag cgacatacca attgggttag ggcgaaaagt ggagcgtgtt
    61621 gcgaaaaccc ccattctggc attaccgcca agacgaggcg gacgcacagt cggacgccag
    61681 ccgccttcca tcgtttgatg aaagacgaaa agtatccata aatggctgaa cgcttccagg
    61741 ccgcgaacgg cgtcggcttg gttgtaggga gcaatgagat gcagttcgcc gttggcgctt
    61801 tttaccagac ctggttggcg cggaacggcg aatttttctt tatagggcga gcgaataacg
    61861 cctatttgct cgaactggaa actgctcatt tcgccgtaat gttaagcgca gaaccgatac
    61921 atacagcctg acgatagcag cctggcgtac cgctggtgac ttcgcagcta tgcagtaata
    61981 ccgcattggc tttcattttc gaggcgttga tttgcatccg cttacgcgcg gttggaatgc
    62041 tcggcggaga gtcctgatta gaggcttggc aagagtcgcc actgacttca ccgagatcgc
    62101 ggaacggttt gccgactaat tcttctgcat tggtataaat tcggaccggc gtggcgcgcg
    62161 gcactttcgg ttttgcagac tccgctttcg gctggggtgc agtgctttga acgggttcga
    62221 caggggatct gcttaacatg gaacagccgc ttagcatgag tgctactaaa cagatcggta
    62281 aagcacgcat agtatttcct caatgtatga tcaaaacgtc aatattgaat caggagcttg
    62341 taaaaatgac aagacgggca agcgcccgtc ctgaatgata ttacaaattg tggaaacagc
    62401 ctaaaaatta ccagccttta acagcgccgc cgttaaacac tttgtttgct gcttcgtaaa
    62461 cttcgtcaga ctgataagcc tggacgaatt tcttcacgtt ctcggcgtct ttgttatctt
    62521 cacgcgttac gatcaggttt acgtacgggg actctttatc ttcgacaaag ataccgtctt
    62581 tcgccggagt caggccaatc tggctggcat aggtggtatt gataactgcc agagcgattt
    62641 gcgcgtcgtc cagagaacgc ggcagctgcg gtgcttccag ctcaacaatt ttcagatttt
    62701 tggggttctc aacaacatca agaacggtcg gcagcaggcc aacgccatct ttcagtttga
    62761 tcaagcccac tttttgcagc agcagcagtg aacgaccaag gttagttggg tcgtttggca
    62821 cggcaacctg cgaaccatcc tgcagttcat ccagtgattt gattttcttg gagtaacctg
    62881 caatcggata aacaaaagtg ttgcctactg cgaccagttt gtagccacga tctttcagtt
    62941 gctgatcaag gtacggttta tgctggaagg cgttggcgtc gatatcgcct ttgctcaatg
    63001 cttcgtttgg cagaacatag tcgttgaatg ttaccagctc aacgtccagg ccatatttgt
    63061 ctttcgcaac tttctgcgcg acttctgcaa cctgctgttc ggcaccaaca atcacgccga
    63121 ctttaatgtg gtttggatct ttttcatcct gaccgcagcc taccagtgcc agtgatccaa
    63181 tcagggctcc cactgccgca aaggttttga atttgaacgc cataccttat tccttcttca
    63241 attatttatg ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg cggtcgcctg
    63301 cgaactgaat taaataaacc agaatgacca gcaataccag taccgtattc atcaccgtcg
    63361 cgttgtagcc gatgtagcca tactgatagc caatctgacc taaaccaccg gcaccgactg
    63421 caccacccat cgcggaataa ccgaccaggg taatcagggt gatagttgcc gcatttacca
    63481 gacccggcaa cgcttccggt aacagcactt tacggacgat ctgcattggc gtggcaccca
    63541 ttgcgcggga agcttcaatt aacccggttg ggatctccag cagagcgttc tcgaccatac
    63601 gggcaataaa cggtgctgca ccaacggtta acggaacaat cgctgcctgc aaaccaatcg
    63661 atgtaccaac aataacgcgg gtaaacggaa tcatccatac cagcaagata atgaacggga
    63721 tggaacggaa aatgttcaca atcgcagaaa cggtacgata cagcttcgcg ttagcaataa
    63781 tttgccccgg acgcgtgaca taaagcagga cgccaaccgg cagaccaatc acaaatccaa
    63841 aaaaaccgga tacgaaggtc attgccagcg tttcccatac gccacgaacc agcagccaca
    63901 tcatcggctc agacataacc cagtacctct acttttacat ggtgttcctg cagccaggta
    63961 atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag tcagcatgat gccgaactta
    64021 acgccaccgg cgtaatccat ctgcgcgcta ataatgttgt tgttgacgtt gaaacgacgc
    64081 gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg taaactccag acgcaacatc
    64141 ggcacgcagt cagtgaatgg ctccgcttgc agacgttcct ggtaatcttc cgggatatcc
    64201 agatgcaggg tcgactgaat aaacttctgc gccagcggcg ttttcggatg cgagaacact
    64261 tcacttaccg tgtcctgctc gatcagttcg ccattgctga tgaccgccac gcaatcacaa
    64321 atacgcttca caacgtccat ttcgtgggtg atcaacagaa tcgtcaaacc cagacggcgg
    64381 ttgatgtctt tcagcagttc gagaatagaa cgtgtcgttg ccggatccag cgcgctggtg
    64441 gcctcatcac acagcaatac tttgggattg ctggctaacg cacgggcaat cgccacacgc
    64501 tgtttctgcc caccggaaag attcgacggg tagctatcat gcttatcgcc aagaccaacc
    64561 aatgacagca attccgtcac gcgacgtttg atctcatctt tcggtgtgtt gtccagctcc
    64621 agcggcagag ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa atgctggaaa
    64681 atcataccaa tctggcggcg agctttggtc aactcggatt ctgacagcgt ggtcagttcc
    64741 tggccatcga ccagcacgct accctcggtt gggcgctcca gcaggtttac acaacgtata
    64801 agcgtactct tacccgcgcc agaggcaccg ataacgccat aaatttgtcc agctggcaca
    64861 tgcaggctga cgttgttcaa cgcctggatg gtgcgggtgc cctggtggaa cactttggtg
    64921 atattcgaaa gttttatcat tgattattta ttatcgtcat taagttagtc gtggcatttc
    64981 gaatgcctgg aacgggcaac gccttcaatg aaatggatgt taaggcatcc agacgtctaa
    65041 atcaatcagg tttatgcgaa gagcactttc ttgctggtcg aaacatgcga tactagcgtc
    65101 acatgcctta ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat ttttcttgac
    65161 cgtgatggca ccattaatgt cgatcacggc tatgtccatg agatcgacaa ctttgaattt
    65221 atcgacggtg ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc gctggtggta
    65281 gtaaccaacc agtctggcat tgctcgcggt aaatttactg aagcacagtt tgaaacgctg
    65341 accgagtgga tggactggtc gctggcggac cgcgatgtcg atctggatgg tatctattat
    65401 tgcccgcatc atccgcaggg tagtgttgaa gagtttcgcc aggtctgcga ctgccgcaaa
    65461 ccacatccgg ggatgctttt gtcagcacgc gattatttgc atattgatat ggccgcttct
    65521 tatatggtgg gcgataaatt agaagatatg caggcagcag ctgcggcgaa cgtgggaaca
    65581 aaagtgctgg tgcgtacggg taaacctgtt acgcctgaag cagaaaacgc ggcggattgg
    65641 gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa accggcgtaa
    65701 tgattaaaag atgagcggtt gaaataaaaa tgcatttttc cgcttgtctt cctgagccga
    65761 ctccctataa tgcgcctcca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    65821 ttcggttaaa gagaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
    65881 tacgccacct cgcgacagtg agctgaaagc cgcgtcgc
//
LOCUS       DAZUCC010000002        61144 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZUCC010000002 DAZUCC010000000
VERSION     DAZUCC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.002"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            291..1232
                     /gene="psuK"
                     /locus_tag="ACHY09_000062"
     CDS             291..1232
                     /gene="psuK"
                     /locus_tag="ACHY09_000062"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFS3883088.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            1220..2158
                     /gene="psuG"
                     /locus_tag="ACHY09_000063"
     CDS             1220..2158
                     /gene="psuG"
                     /locus_tag="ACHY09_000063"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFS3883089.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELAHTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            2252..3502
                     /locus_tag="ACHY09_000064"
     CDS             2252..3502
                     /locus_tag="ACHY09_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFS3883090.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(3570..4268)
                     /gene="yeiL"
                     /locus_tag="ACHY09_000065"
     CDS             complement(3570..4268)
                     /gene="yeiL"
                     /locus_tag="ACHY09_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFS3883091.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            4398..5339
                     /gene="rihB"
                     /locus_tag="ACHY09_000066"
     CDS             4398..5339
                     /gene="rihB"
                     /locus_tag="ACHY09_000066"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFS3883092.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5439..6689
                     /gene="nupX"
                     /locus_tag="ACHY09_000067"
     CDS             5439..6689
                     /gene="nupX"
                     /locus_tag="ACHY09_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFS3883093.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKSFINRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(6745..7833)
                     /gene="yeiI"
                     /locus_tag="ACHY09_000068"
     CDS             complement(6745..7833)
                     /gene="yeiI"
                     /locus_tag="ACHY09_000068"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFS3883094.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(7836..8693)
                     /gene="nfo"
                     /locus_tag="ACHY09_000069"
     CDS             complement(7836..8693)
                     /gene="nfo"
                     /locus_tag="ACHY09_000069"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS3883095.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTSAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(8767..9816)
                     /gene="yeiH"
                     /locus_tag="ACHY09_000070"
     CDS             complement(8767..9816)
                     /gene="yeiH"
                     /locus_tag="ACHY09_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFS3883096.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            9915..10796
                     /gene="yieE"
                     /locus_tag="ACHY09_000071"
     CDS             9915..10796
                     /gene="yieE"
                     /locus_tag="ACHY09_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFS3883097.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            11001..12470
                     /gene="lysP"
                     /locus_tag="ACHY09_000072"
     CDS             11001..12470
                     /gene="lysP"
                     /locus_tag="ACHY09_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFS3883098.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAVS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            12763..14742
                     /gene="cirA"
                     /locus_tag="ACHY09_000073"
                     /pseudo
     CDS             12763..14742
                     /gene="cirA"
                     /locus_tag="ACHY09_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(14774..15610)
                     /gene="yeiG"
                     /locus_tag="ACHY09_000074"
     CDS             complement(14774..15610)
                     /gene="yeiG"
                     /locus_tag="ACHY09_000074"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFS3883099.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            15868..16536
                     /gene="folE"
                     /locus_tag="ACHY09_000075"
     CDS             15868..16536
                     /gene="folE"
                     /locus_tag="ACHY09_000075"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFS3883100.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            16553..17694
                     /gene="yeiB"
                     /locus_tag="ACHY09_000076"
                     /pseudo
     CDS             16553..17694
                     /gene="yeiB"
                     /locus_tag="ACHY09_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
     gene            17836..18876
                     /gene="galS"
                     /locus_tag="ACHY09_000077"
     CDS             17836..18876
                     /gene="galS"
                     /locus_tag="ACHY09_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFS3883101.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            19156..20154
                     /gene="mglB"
                     /locus_tag="ACHY09_000078"
     CDS             19156..20154
                     /gene="mglB"
                     /locus_tag="ACHY09_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFS3883102.1"
                     /translation="MNKKVLTLFAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            20215..21735
                     /gene="mglA"
                     /locus_tag="ACHY09_000079"
     CDS             20215..21735
                     /gene="mglA"
                     /locus_tag="ACHY09_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFS3883103.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            21751..22761
                     /gene="mglC"
                     /locus_tag="ACHY09_000080"
     CDS             21751..22761
                     /gene="mglC"
                     /locus_tag="ACHY09_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFS3883104.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(22832..24067)
                     /gene="preA"
                     /locus_tag="ACHY09_000081"
     CDS             complement(22832..24067)
                     /gene="preA"
                     /locus_tag="ACHY09_000081"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFS3883105.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(24061..25299)
                     /gene="preT"
                     /locus_tag="ACHY09_000082"
     CDS             complement(24061..25299)
                     /gene="preT"
                     /locus_tag="ACHY09_000082"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFS3883106.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLKGACSC"
     gene            complement(25618..25857)
                     /gene="yeiS"
                     /locus_tag="ACHY09_000083"
     CDS             complement(25618..25857)
                     /gene="yeiS"
                     /locus_tag="ACHY09_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFS3883107.1"
                     /translation="MDVQQFFVVAVFFLIPVFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(25860..26579)
                     /gene="sanA"
                     /locus_tag="ACHY09_000084"
     CDS             complement(25860..26579)
                     /gene="sanA"
                     /locus_tag="ACHY09_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFS3883108.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(26729..27613)
                     /gene="cdd"
                     /locus_tag="ACHY09_000085"
     CDS             complement(26729..27613)
                     /gene="cdd"
                     /locus_tag="ACHY09_000085"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS3883109.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALVAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(27743..28438)
                     /gene="yohK"
                     /locus_tag="ACHY09_000086"
     CDS             complement(27743..28438)
                     /gene="yohK"
                     /locus_tag="ACHY09_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFS3883110.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(28435..28833)
                     /gene="yohJ"
                     /locus_tag="ACHY09_000087"
     CDS             complement(28435..28833)
                     /gene="yohJ"
                     /locus_tag="ACHY09_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS3883111.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            complement(28964..29872)
                     /locus_tag="ACHY09_000088"
     CDS             complement(28964..29872)
                     /locus_tag="ACHY09_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3883112.1"
                     /translation="MANWAQKLKLHHLQMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGLTLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLKEMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYRVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            29999..31357
                     /locus_tag="ACHY09_000089"
     CDS             29999..31357
                     /locus_tag="ACHY09_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3883113.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAIIRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAVCIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTQLITVVRQSTSVPDSLPHAGVV
                     NE"
     gene            31369..32397
                     /gene="gtdA"
                     /locus_tag="ACHY09_000090"
     CDS             31369..32397
                     /gene="gtdA"
                     /locus_tag="ACHY09_000090"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFS3883114.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTSMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVTIGNETFSFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            32413..33114
                     /locus_tag="ACHY09_000091"
     CDS             32413..33114
                     /locus_tag="ACHY09_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFS3883115.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAIVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEN
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKASETGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGSVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            33122..33766
                     /gene="maiA"
                     /locus_tag="ACHY09_000092"
     CDS             33122..33766
                     /gene="maiA"
                     /locus_tag="ACHY09_000092"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFS3883116.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLESHYPQVPLMPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            33781..34974
                     /locus_tag="ACHY09_000093"
     CDS             33781..34974
                     /locus_tag="ACHY09_000093"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFS3883117.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARQGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEIARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWIGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPCCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            35036..35983
                     /gene="dusC"
                     /locus_tag="ACHY09_000094"
     CDS             35036..35983
                     /gene="dusC"
                     /locus_tag="ACHY09_000094"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFS3883118.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPAGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(36356..36439)
                     /gene="yohP"
                     /locus_tag="ACHY09_000095"
     CDS             complement(36356..36439)
                     /gene="yohP"
                     /locus_tag="ACHY09_000095"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFS3883119.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            36663..38099
                     /gene="mdtQ"
                     /locus_tag="ACHY09_000096"
     CDS             36663..38099
                     /gene="mdtQ"
                     /locus_tag="ACHY09_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFS3883120.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQHTPAVQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPVMQVAEQRIQLAEAQAKAIATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVDAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            38152..38913
                     /gene="yohF"
                     /locus_tag="ACHY09_000097"
     CDS             38152..38913
                     /gene="yohF"
                     /locus_tag="ACHY09_000097"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3883121.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGARAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVRPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(39032..39610)
                     /gene="yohD"
                     /locus_tag="ACHY09_000098"
     CDS             complement(39032..39610)
                     /gene="yohD"
                     /locus_tag="ACHY09_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFS3883122.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILAVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39780..40367
                     /gene="yohC"
                     /locus_tag="ACHY09_000099"
     CDS             39780..40367
                     /gene="yohC"
                     /locus_tag="ACHY09_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFS3883123.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            40541..41473
                     /gene="pbpG"
                     /locus_tag="ACHY09_000100"
     CDS             40541..41473
                     /gene="pbpG"
                     /locus_tag="ACHY09_000100"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFS3883124.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(41512..43227)
                     /gene="dld"
                     /locus_tag="ACHY09_000101"
     CDS             complement(41512..43227)
                     /gene="dld"
                     /locus_tag="ACHY09_000101"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFS3883125.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGVGKTSKRKN
                     WQEVE"
     gene            43423..45720
                     /gene="bglX"
                     /locus_tag="ACHY09_000102"
     CDS             43423..45720
                     /gene="bglX"
                     /locus_tag="ACHY09_000102"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFS3883126.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATAKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45869..46786
                     /gene="osmF"
                     /locus_tag="ACHY09_000103"
     CDS             45869..46786
                     /gene="osmF"
                     /locus_tag="ACHY09_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFS3883127.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            46793..47950
                     /gene="yehY"
                     /locus_tag="ACHY09_000104"
     CDS             46793..47950
                     /gene="yehY"
                     /locus_tag="ACHY09_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFS3883128.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACVIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHLLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGISGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            47943..48869
                     /gene="yehX"
                     /locus_tag="ACHY09_000105"
     CDS             47943..48869
                     /gene="yehX"
                     /locus_tag="ACHY09_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFS3883129.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLVEA"
     gene            48874..49605
                     /gene="yehW"
                     /locus_tag="ACHY09_000106"
     CDS             48874..49605
                     /gene="yehW"
                     /locus_tag="ACHY09_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFS3883130.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKSMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(49586..49693)
                     /gene="yohO"
                     /locus_tag="ACHY09_000107"
     CDS             complement(49586..49693)
                     /gene="yohO"
                     /locus_tag="ACHY09_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFS3883131.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49753..50484)
                     /gene="mlrA"
                     /locus_tag="ACHY09_000108"
     CDS             complement(49753..50484)
                     /gene="mlrA"
                     /locus_tag="ACHY09_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFS3883132.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            50706..52391
                     /gene="btsS"
                     /locus_tag="ACHY09_000109"
     CDS             50706..52391
                     /gene="btsS"
                     /locus_tag="ACHY09_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFS3883133.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDCYTRITLRLPWRDEA"
     gene            52388..53107
                     /gene="btsR"
                     /locus_tag="ACHY09_000110"
                     /gene_synonym="yehT"
     CDS             52388..53107
                     /gene="btsR"
                     /locus_tag="ACHY09_000110"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFS3883134.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            53154..53624
                     /gene="yehS"
                     /locus_tag="ACHY09_000111"
     CDS             53154..53624
                     /gene="yehS"
                     /locus_tag="ACHY09_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS3883135.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(53666..54127)
                     /gene="yehR"
                     /locus_tag="ACHY09_000112"
     CDS             complement(53666..54127)
                     /gene="yehR"
                     /locus_tag="ACHY09_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFS3883136.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(54252..56255)
                     /gene="yehQ"
                     /locus_tag="ACHY09_000113"
     CDS             complement(54252..56255)
                     /gene="yehQ"
                     /locus_tag="ACHY09_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS3883137.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIDGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGKCLCQLAALTSRLNSAQEIARRDSVGEVPPMPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVEGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEHSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(56252..57388)
                     /gene="yehP"
                     /locus_tag="ACHY09_000114"
     CDS             complement(56252..57388)
                     /gene="yehP"
                     /locus_tag="ACHY09_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFS3883138.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGDSNLTTPEWINSIHTLFPQRVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSDQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(57381..59660)
                     /gene="yehM"
                     /locus_tag="ACHY09_000115"
     CDS             complement(57381..59660)
                     /gene="yehM"
                     /locus_tag="ACHY09_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFS3883139.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPYAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREDFMARWIGWAMQQNNGDVLVVCGGWHASALAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWRWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISFPAE
                     LTLNRFTPDGLAQSQVLHRLVILEIPGVVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFNQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLVQLSPTQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(59671..60759)
                     /gene="yehL"
                     /locus_tag="ACHY09_000116"
     CDS             complement(59671..60759)
                     /gene="yehL"
                     /locus_tag="ACHY09_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFS3883140.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT        14508 a        15360 c        16387 g        14889 t
ORIGIN      
        1 caaacactaa ggttatttcc tcaagggcag attattttct gccctttttt agtgttcctg
       61 ttctgtgcat cccgtcacaa attcctcact tttgtaattc aggcatttaa tcgcattttg
      121 tgagcctgct caaatctgcc cattttgcct ctctggcggc aatttacttt acgtaaaaca
      181 tgcatactat gagcacatgt ttaaatatga acacatgttt aaaccgtctt tgcagtatct
      241 atattcttgt ggattgacag gattggtaat tttttaccag aggacgtgct atgcgcgaaa
      301 aggattatgt cgtaattata ggttcggcga atattgatgt cgccggatat tcacatgaat
      361 cattaaatta tgcggattca aatccaggta aaataaagtt tacgcctggt ggagtagggc
      421 gcaatattgc acaaaacctg gcgttgctgg ggaataaagc ctggctactg agcgccgtag
      481 gcagtgattt ttatggtcaa tcgctgctaa cgcaaaccaa tcaatctggt gtttatgtcg
      541 ataaatgcct gattgtgccg ggagaaaata cgtcgagcta tttatcatta ctcgataata
      601 ccggtgaaat gctggttgct ataaatgaca tgaatattag caacgctatt acagctgaat
      661 atctcgcaca gcacggtgaa tttattcaga gggcaaaggt cattgtcgcg gactgtaata
      721 tcagtgaaga ggcactggca tggattctgg ataatgccgc caacgtaccc gtatttgtcg
      781 atcctgtttc cgcgtggaaa tgtgtcaaag tgcgcgaccg tctaaatcaa atccacactc
      841 tcaagccaaa ccgccttgaa gcggaaaccc tgagtgggat tgctttgtca gggcgtgaag
      901 atgtggcaaa agttgctgcc tggttccatc aacatggcct gaaccgactg gtattgagca
      961 tgggcggcga cggcgtttat tacagtgata tcagcggtga aagtggttgg tctgcgccga
     1021 tcaaaaccaa tgtcattaat gttaccggag cgggcgatgc catgatggcg ggacttgctt
     1081 cgtgttgggt agacggaatg ccgtttgccg aatctgttcg tttcgcacag ggatgttcgt
     1141 caatggcgct ctcctgtgaa tacaccaata accccgattt atcgattgcc aacgttatat
     1201 cgttagtgga gaacgcagaa tgtctgaatt aaaaatttcc cctgaattat tacaaatttc
     1261 cccggaagtg caggacgctt taaaaaacaa aaaaccggtt gtggcgctgg aatcgaccat
     1321 tatttctcac gggatgccgt tcccacaaaa tgcccagacc gcaattgaag ttgaagaaac
     1381 tattcgtaaa cagggcgctg tacctgccac tatcgccatt attggcggcg tgatgaaagt
     1441 gggtttaagc aaagaagaaa ttgaattgct gggtcgtgaa gggcataacg tgaccaaagt
     1501 tagtcgtcgc gatttacctt ttgttgttgc cgccggaaaa aatggtgcaa ccaccgttgc
     1561 ttcaacgatg attattgcgg cgcttgccgg aattaaagta tttgccaccg ggggaattgg
     1621 tggtgtgcat cgcggggcgg aacatacctt cgatatttct gccgatttgc aagaactggc
     1681 gcacaccaat gtcaccgtcg tttgtgccgg ggcgaaatct attctcgatt taggcttaac
     1741 taccgaatat ttagaaactt tcggtgtacc gctaattggc tatcagacca aagcgctgcc
     1801 tgcgtttttc tgccgcacca gcccgtttga cgtgagcatt cgtctcgaca gcgccagcga
     1861 aattgcccgt gcaatggcgg tgaaatggca aagcgggctg aacggtggcc tcgtggtagc
     1921 gaacccgatc ccggaacagt ttgctatgcc ggaacacact atcaatgcgg cgatcgatca
     1981 ggcggtagct gaagctgaag cgcagggtgt tattggtaaa gaaagtacgc cattcctgct
     2041 ggcgcgcgtt gctgagttga ccggcggtga cagcctgaaa tccaacatcc agctggtgtt
     2101 caacaacgcc atcctggcga gtgaaatagc caaagaatac cagcgtctcg cgggttaatt
     2161 tctgacgggc agaaagccgt cgcccgtact catcaagcat accaaaacct ggcgctgacg
     2221 ccgggtttac tggcatgaag ggaaaatcat tatggatata atgagaagtg ttgtggggat
     2281 ggtggtgtta ctggcaatag catttctgtt gtcagtgaat aaaaagagca tcagtttgcg
     2341 cacggttgga gccgcactgc tgctgcaaat cgctattggt ggcatcatgc tctacttccc
     2401 accgggaaaa tgggcagtag aacaggcggc attaggcgtt cataaagtga tgtcttacag
     2461 tgatgccggt agcgccttca tttttggttc gctggtcggg ccgaaaatgg atgtcctctt
     2521 tgacggtgcg ggttttatct tcgcctttcg cgtacttccg gcgattatct tcgtcactgc
     2581 gctcatcagt ctgctgtact acattggcgt gatggggctg ctgattcgca tccttggcag
     2641 cattttccag aaagccctca acatcagcaa aatcgaatct tttgttgcgg ttaccactat
     2701 tttcctcggg caaaatgaga tcccggcgat cgttaaaccg tttatcgatc gcatgaatcg
     2761 caacgagttg tttaccgcaa tttgtagcgg gatggcgtcc attgctggtt cgatgatgat
     2821 tggttatgcc ggaatgggcg taccaattga ctacctgtta gcggcatcgc tgatggcgat
     2881 ccctggtggt attttgtttg cacgtattct tagcccagcc accgagcctt cgcaggtgac
     2941 atttgaaaat ctgtcgttca gcgaaacgcc gccaaaaagc tttatcgaag cggcggcgag
     3001 cggtgcgatg accgggctga aaatcgccgc tggtgtggcg acggttgtaa tggcgtttgt
     3061 cgcaattatt gcgctgatca acggcattat cggcggaatt ggcggctggt ttggtttcgc
     3121 caatgcctct ctggaaagta tttttggcta tgtgctggca ccgctggcgt ggatcatggg
     3181 tgtggactgg agtgatgcca atcttgcggg tagcctgatt gggcagaaac tggcgattaa
     3241 cgaattcgtc gcttacctga gtttctcccc atacctgcaa acgggcggca cgctggaagt
     3301 gaaaaccatt gcgattatct cctttgcgct ttgtggtttt gctaactttg gttctatcgg
     3361 tgttgtcgtt ggggcatttt cggctatttc gccaaaacgc gcaccggaaa tcgcccagct
     3421 tggtttacgg gcgctggcag cagcaacgct ttccaacctg atgagcgcga ccattgccgg
     3481 gttctttatt ggtttagctt gagtgtcatt gccggatgta cgcgcttatt aagcgtcaca
     3541 tccggcaatc gctctattgc aaataatttt tactgcatca tccctgagaa tttattttcc
     3601 gggtccatct ccagtgccag tcctgacaac tgctttctgt ttttaatgag ataccctttc
     3661 ttgcttttaa ttaataaacc gtcgtgaatg aattgcgcga gaacatataa aagatgtcga
     3721 taagaaacac ctaaatactc tgccgcttgc gtatgctttt cgtgataaag atcaccttcc
     3781 tgcgagagta atataaaagc tgccaggcga ttcactaacg gaaatgattg attctgagtt
     3841 aaagaaacaa tattacgata atttttatga cttaaggtga cgcagagttt tcgtaaaaat
     3901 agcgtgtcgt ttaataacag cgggcggtaa tgtttcatag ggagcgcaag gcaccaacac
     3961 tcttcaatag cctgcaccgc acgcggttca tggtctttgt cgattaactc aatctcgcca
     4021 ataaaacagg gagcggcaaa gaaatcgatc agcgacacgc gaccattagc aagcgtggcg
     4081 taaagcctgg cacggcctcg cgtcagataa aacagccatg acggctgttg cccttcctgc
     4141 acaatgtagt cacgcgctaa aaaatgaaac agccgcgtat cggctgaaac atccgttgaa
     4201 aagcaatcct taaacgcaga ttcactcatc aattgctgct taaggtcatt attgtggatt
     4261 tctttcacga tgtttatccg ctatgagatt tctcatattt agcatactta cgctgttgtt
     4321 aagattcaat catcggcccg gtcgaatttt ctttgctgta gaaaaaataa cgcaactgga
     4381 aacagaggaa ataaacaatg gaaaagagaa aaattattct ggattgtgat ccgggtcatg
     4441 atgatgctat tgccataatg atggcagcga aacatccggc gattgattta ttaggtatca
     4501 ccattgtggc gggtaatcag acgcttgata aaacattaat taatggcctg aatgtttgcc
     4561 agaaactgga gattaatgtt ccggtttatg cggggatgcc acagcccatt atgcgtcaac
     4621 aaatcgttgc cgataatatt cacggtgaaa ccggactgga tggcccggta ttcgagccgc
     4681 tgacccgcca ggcagaaagc actcatgcgg tgaaatatat cattgatacc ctgatggcaa
     4741 gcgatggcga tatcactttg gtgccggttg gcccactttc aaatatcgcg gtggcaatgc
     4801 gtatgcaacc cgcgatcctg ccgaaaatcc gtgaaattgt gctgatgggc ggcgcttacg
     4861 gtactggcaa cttcacccca tctgccgagt tcaacatctt tgctgacccg gaagccgcac
     4921 gcgtggtgtt cacctccggc gttccattag tgatgatggg cctcgatctc accaaccaga
     4981 ccgtttgcac cccggacgtg attgctcgga tggaaagggc aggcggccct gccggagagc
     5041 tgttcagcga catcatgaac ttcactctca aaacccagtt cgaaaactac ggcctggctg
     5101 gcggcccggt gcatgacgcc acctgcatcg gttatctgat taaccctgat ggcattaaaa
     5161 cccaggagat gtacgtcgaa gtggacgtta acagcggccc ttgctacggg cgtacggtct
     5221 gcgacgagct gggcgttctt ggcaagcccg ccaataccaa agtcggcatc actattgata
     5281 ctgactggtt ctggggatta gtcgaagagt gcgtgcgcgg ctacatcaaa acccattaag
     5341 tgttgataac aagccgggac agtatcgcaa gatgcaggcc cggctatttc tgatgggcag
     5401 aaagccgtcg cccaaaatga catgaagaga taataactat ggatgtcatg agaagtgttc
     5461 tgggaatggt ggtattgctg gcaattgcgt ttttgctgtc agtaaacaag aagaagatca
     5521 gcctgcgtac cgttggcgcg gcgttagtgt tacaggtcgt gattggcggc attatgcttt
     5581 ggttaccacc agggcgttgg gtcgcagaaa aagtcgcttt tggcgtgcat aaagtgatgg
     5641 cgtacagcga tgcaggtagc gcatttatct ttggttctct ggtcggacca aaaatggata
     5701 ccttatttga tggcgcagga tttatctttg gtttcagggt attaccggca attatcttcg
     5761 ttacagcact ggtgagtatt ctctactaca tcggtgtgat ggggatctta attcgcattc
     5821 tcggcggtat cttccagaaa gcattgaata tcagcaagat cgagtcattc gtcgcggtca
     5881 ctaccatttt cctcgggcaa aacgaaattc cggcaatcgt caaatccttt atcaatcgtc
     5941 tgaatcgcaa tgaattattt acagcgattt gtagcgggat ggcctcgatt gctggttcga
     6001 caatgattgg ttacgccgca ctgggcgtgc ctgtggaata tctgctggcg gcatcgttaa
     6061 tggcgatccc cggcgggatc ttgtttgccc gcctgttaag cccggcaaca gaatcttcgc
     6121 aggtttcctt taataacctc tctttcaccg aaacaccgcc aaaaagcatt attgaagccg
     6181 ctgcgacagg ggcaatgacc gggctgaaaa tcgccgcagg tgtggcgaca gtagtgatgg
     6241 catttgtcgc catcattgcg ttaattaacg gtattatcgg cggcgttggc ggctggtttg
     6301 gctttgaaca tgcctcgctg gagtccattt taggttacct gctggcccca ctggcgtggg
     6361 taatgggtgt tgactggagt gatgcgaatc ttgccgggag tttgattgga cagaaactgg
     6421 caataaatga atttgtcgct tacctcaatt tctcacctta tctgcaaaca gctggcactc
     6481 tggatgctaa aaccgtggcg attatttcct tcgcgttgtg cggtttcgct aactttggtt
     6541 ctatcggggt ggtggtgggg gcgttttctg cggttgcgcc acaccgtgca ccggaaatcg
     6601 cccagcttgg tttacgcgcg ctggcggcgg cgacactttc taacctgatg agtgcaacca
     6661 ttgcaggatt ctttattggc ttagcgtagt gcgcgagtta tgagccggat aagatgtagt
     6721 caagcgtcga atccggcaac attgttacac catcggcact aatgaaagcg cgttatcggc
     6781 agacagggta gggttgttta agctgccgct ggcgcgtgaa attgccgcgc aggccatcgc
     6841 aaaacgggcg ctgtcacgga aattgtttcc ctccagaaag ctatatacca ggcccgccat
     6901 aaaaccatcg tcagcaccaa aactgtctac tgtcgtatgc gctggcgctg tcagcaaaaa
     6961 ttgctctcca tccttttcgc tgcaataaac tgactcatcg ggcaaataaa caaacagttg
     7021 ctgaacgcct tgctgatgca acgcattcac tgcggcatta cggtcagcat cgctggtgat
     7081 cgcctgtccc cataaaattt ccagctccgg taaagtgggt ttcagggtgt gaatatgcgc
     7141 cagccagtgt ttgattttgc ccgctttgaa ttctgaaacg gtatcgacaa acaccgggat
     7201 ttcatcagcg agggtaaaga cccattccag cgcctcggct gtcaggttac aatcagcaag
     7261 taccacgccc gcatgacgaa gtaaatcgcg cgacccgttc agtagttgcg gtgtcaactg
     7321 atccagcaga tgggtatcgt taatcgccag cacggtttga tcgtctcgat tggcaattgc
     7381 cagatacgtc gatgtgcttt ggccatgtaa acgaacgcag ccggagacat tcacgccggc
     7441 gcggcgcgtt tcttccagga gcatttcgcc ataaaagtca tcaccaatca ctgaaagcaa
     7501 atggacgtcg cggcctaaca gcgccaggtt gtgggcgata ttgcgtccaa cgccgcctgc
     7561 cgagcaatga atggtaccgg gatgagaagc cgcttgcggg taacggatat ccgccatccc
     7621 gcgaatatcc atattgattg tccccaccac tacgcagtat tcctgctcgg tgagaatgta
     7681 acctttgcct ttaatccggc ctttgcgcat taaatccata atatgcgccg caacgcgcga
     7741 acggctgatt tgcagcatat ccgcaatttc gttctgctga atcagcgggt tacgccgtaa
     7801 aattgcaagg atctccttct cccggttgtt catcttcagg ctaccgcttt ttcagtttgt
     7861 tgcgctttca gccaggcgat ctcttctgcc cagatatccg ggttgatggt ttcaaggatc
     7921 agcggaatgc cgtcgaaacg gtcgtcctgc atgatccagc ggaacgcatc atgaccaata
     7981 ttgccttcac cgaggctatg atggcggtca acgcggctgc caaaggtgct tttcgcatcg
     8041 ttaaggtgca tcccgcgcag atacttaaag ccaacaatac gggcaaaatc cgcgaacgtt
     8101 ttctcgcatt cggcagaagt acgcaaatca tacccggcag caaaagcatg gcaggtatca
     8161 atgcagacgc cgacgcggga tttatcttcc acgccgtcga taatcgccgc gagatgttcg
     8221 aatttaaacc ctaagttact gccctgaccg gcggtgtttt caatcaccgc tgtcacacct
     8281 tgagttttat ccagcgcaat gttgatggat tcggcaatac gcgcaaggca atcctcttct
     8341 gaaatctgca tcagatggct gccagggtgg aagttgagca aagaaagccc cagctgttcg
     8401 caacgctgca tttcatctat aaacgcatcg cgcgattttt ccagagcttc agcgaccgga
     8461 tgtccgaggt taatcagata actgtcgtgg ggaagaattt gcgccgatgt gtagtgatat
     8521 ttttcacagg cggctttgaa ttcatcgatg gtttgcgtcg tgagcggtgc ggcacgccac
     8581 tgacgttggt ttttggtgaa caaggcaaac gcggttgcgt cgatttcggc ggcgcgaatt
     8641 gcggcatttg ccagaccgcc agcagcacta acgtgcgctc caatgtattt catgcgagga
     8701 ctcctgttaa acccgctgga ggaaaacggt aatgatagcg ggttaacagg agcaagatgt
     8761 agtggtttat gcgatgacgc tttgaatcac atagttaatc gcaccaccac caacaatcag
     8821 ccaggcaaac agtaccagtg ccatcagcag aggtttcgcc ccggcttttt tcagcgcgct
     8881 gacgtgagtg gtcagaccca gcgccgccat cgccattgcc agcaggaagg tatccagcgt
     8941 taccagcatg ttcaccacgc tctgcggtaa caggtggaac gagttaaaga tggcaactac
     9001 gatgaacaag atggcaaacc acggaatagt gattttgctt ttctcgccgc tgttcgcccc
     9061 agacagctgt ttaacacgcg ccgccagcag gatgaggaac ggagccagca tcatcacgcg
     9121 cagcattttg gaaataactg ctgcgttttc tgcatccggg ctgatggcat gacctgccgc
     9181 caccacctgc gccacttcgt gcacagtaga accaatgtag ataccgaaag tttccggact
     9241 aaaccattga gacatcagcg gatatatcgc cgggtagagg aaaatcgcga cggtcccgaa
     9301 gataacaacg gttgcaacag ccacggttac tttactggct tccgctttca ctaccggctc
     9361 agtcgccagt accgcggcag caccacagat actgctaccg gcaccgatca accagctggt
     9421 gtgcttatcc agaccaaaca ctttctgccc caggaagcaa gccagcagga aggtactgga
     9481 cagcgtcagc acgtcaatga tgatcccact gataccgaca tcggcaattt gcgagaacgt
     9541 cagacggaag ccataaagaa tgatacccag acgtaataaa tattgcttgg caaacagcac
     9601 accaccgtca cagcttttcc agatgtgcgg atagatggtg ttgcctaaaa ccatccccaa
     9661 caagattgcg agggtgaggg cactaaaccc ggcacccgca accgcgggaa tggaaccacc
     9721 ccacagggcg accccggtga taactgcact cagggctaac cccggaataa aatgccacag
     9781 tgtacgatgt tgtttctgta aggtgatatt cgtcataacc ctctccttta accgggcata
     9841 aggttacgac taactggttt aaaaataaaa ttgattatat atttataatt aatctttata
     9901 agtggtaagc gactatgcac atcaccctcc ggcagttgga agtttttgca gaagtattga
     9961 aaagtggatc aaccacccag gcgtcggtga tgctggcgtt gtcgcaatca gcagtgagcg
    10021 cagccttgac cgatctggaa gggcagcttg gcgtgcaact gtttgatcgc gtggggaaaa
    10081 gactggttgt taatgaacac gggcggctgc tctatccgcg cgcgttggca ctgcttgaac
    10141 aggcggttga aatcgaacaa ctgtttcgcg aagacaacgg cgcgattcgt atctatgcca
    10201 gtagtactat cggtaactac attctgcctg cagtcattgc ccgttatcgc catgattatc
    10261 cgcagttgcc gattgaactt agcgttggga acagccagga cgtgatgcaa gcggtgctgg
    10321 atttccgcgt tgatattggc tttattgaag gaccgtgcca cagcactgaa atcatttctg
    10381 aaccgtggct ggaagacgag ctggtggttt tcgccgcgcc gacttcgcca ttggctcgtg
    10441 gtccggtcac cttagaacag ctggccgctg cgccgtggat cctgcgtgaa cgcggctccg
    10501 gcacgcggga gattgtcgat tatctgttgc tgtcacattt accgaagttt gagatggcga
    10561 tggaattagg taactccgag gcaatcaaac atgcggtgcg tcatgggttg ggaattagtt
    10621 gcctgtcgcg acgggtgatt gaagatcaat tgcaggcagg cacattaagt gaagttgcgg
    10681 tccctctgcc gcgcctgatg cgtacgttgt ggcgtataca tcatcggcaa aaacaccttt
    10741 ccaacgcgct acggcgcttt ctggactatt gcgatcccgc gaatgtaccg cgttaagttg
    10801 ctgtacaaga acatgctggt gctgtgtcga tttcgtgacg cagcgccctc agcatgcatt
    10861 cgccagaaaa gagattggct gctttactta taatccctgg gcgatcatga aggtgtctta
    10921 taaccgtgta tttctgccgg aaggattgcc aatcgtctgc tacaatcgcg cctcattttt
    10981 aagatggata gcatttttgt atggtttccg aaactaaaac cacagaagcg ccgggcttac
    11041 gccgtgaatt aaaggcgcgt cacctgacga tgattgccat tggcggttcc atcggtacag
    11101 gtctttttgt tgcctctggc gcaacgattt ctcaggcagg tccgggcggg gcattgctct
    11161 cgtatatgtt gattggcctg atggtttact tcctgatgac cagtctcggt gaactggctg
    11221 catatatgcc ggtttccggt tcgtttgcca cttacggtca gaactatgtt gaagaaggct
    11281 ttggcttcgc gctgggctgg aactactggt acaactgggc ggtgactatc gccgttgacc
    11341 tggttgcagc tcagctggtc atgagctggt ggttcccgga tacaccgggc tggatctgga
    11401 gtgcgctgtt cctcggcgtt atcttcctgc tgaactacat ctcagttcgt ggctttggtg
    11461 aagcggaata ctggttctca ctgatcaaag tcacgacagt tattgtcttt atcatcgttg
    11521 gcgtgctgat gattatcggt atcttcaaag gcgcgcagcc tgcgggctgg agcaactgga
    11581 caatcggcga agcgccgttt gctggtggtt ttgcggcgat gatcggcgta gctatgattg
    11641 tcggcttctc tttccaggga accgagctga tcggtattgc tgcaggcgag tccgaagatc
    11701 cggcgaaaaa cattccacgc gcggtacgtc aggtgttctg gcgaatcctg ttgttctatg
    11761 tgttcgcgat cctgattatc agcctgatta ttccgtacac cgatccgagc ctgctgcgta
    11821 acgatgttaa agacatcagc gtcagtccgt tcaccctggt gttccagcac gcgggtctgc
    11881 tctctgctgc agcggtgatg aacgcagtta ttctgacggc ggtgctgtca gcgggtaact
    11941 ccggtatgta tgcgtctact cgtatgctgt acaccctggc gtgtgacggt aaagcgccgc
    12001 gcattttcgc taaactgtcg cgtggtggcg tgccgcgtaa tgcgctgtat gcgacgacgg
    12061 tgattgccgg tctgtgcttc ctgacctcca tgtttggcaa ccagacggta tacctgtggc
    12121 tgctgaacac ctccgggatg accggtttta tcgcctggct ggggattgcc gttagccact
    12181 atcgcttccg tcgcggttac gtattgcagg gacacgacat taacgatctg ccgtaccgtt
    12241 caggtttctt cccactgggg ccgatcttcg cattcattct gtgtctgatt atcactttgg
    12301 gccagaacta tgaagcgttc ctgaaagata ccattgactg gggcggcgta gcggcaacgt
    12361 atattggtat cccgctgttc ctgattattt ggttcggcta caagctgatt aaaggaactc
    12421 actttgtacg ctacagcgaa atgaagttcc cgcagaacga taagaaataa gttttctccc
    12481 ttccttgcta agccctctca accgagaggg ctttttcaat tccatttccc tgacaaatca
    12541 tgcggatata aaatttaaca tttggattga taattgttat cgtttgcatt atcgttacgc
    12601 cgcaatcaaa aaaggctgac aaatcagagg ctggtctggc tttctgggat gatcacctgc
    12661 atacaaataa gtccaccgcg atgctgccgt acgcaagggg acgtgaagaa gatgtgagcg
    12721 ataacccatt ttattttcgt agttacctca tggagatatg gaatgtttag gttgaaccct
    12781 ttcgtacggg tcgggctgtg tttgtccgct atttcttgtg catggcctgt gttagcggtc
    12841 gatgatgatg gcgaaacgat ggttgtcact gcatcttccg ttgagcaaaa ccttaaagat
    12901 gcacctgcca gtatcagcgt cattacctag gaagacctgc agcgaaaacc ggtacagaat
    12961 ctgaaggatg tcctcaaaga agtgcctggc gtacaactga cgaacgaagg ggataaccgt
    13021 aagggcgtta gtattcgtgg tctggacagc agctacaccc tgattctcgt cgacggtaaa
    13081 cgcgttaact cccgcaatgc cgtcttccgc cacaatgatt tcgatctgaa ctggatcccg
    13141 gtcgattcca tcgaacgtat tgaagtggtc cgtggcccga tgtcgtcgct gtacggttcc
    13201 gatgcgctcg gcggtgtagt gaatatcatc accaaaaaaa tcggtcagaa atggtcgggc
    13261 accgttaccg tcgataccac cgttcaggaa catcgcgatc gcggtgatac ctataacggt
    13321 caattcttta ccagcggacc attaattgac ggcgtgctgg gaatgaaagc ttacggcagc
    13381 ctggcaaaac gtgaaaagga tgacccgcaa aactcaacga ccaccgatac cggagaaacg
    13441 ccgcgtattg aaggattctc cagccgcgac ggcaatgtcg aatttgcctg gacaccgaat
    13501 caaaatcacg attttactgc cggatacggt ttcgaccgtc aggatcgtga ttccgactcg
    13561 ctggacaaaa accgcctgga acgccagaac tactccgtca gccataatgg gcgttgggat
    13621 tacggcacca gcgaactgaa atactacggt gagaaagtcg agaacaaaaa ccctggcaac
    13681 agcagcccga taacttccga aagcaatacg gtcgacggca aatacacgtt gccgctgacg
    13741 gcgattaatc agtttctcac ggttggcggt gaatggcgtc acgacaaact tagcgatgcg
    13801 gtgaacctga ctgggggaac cagctccaaa acatctgcca gccagtacgc gctatttgtt
    13861 gaagatgaat ggcggatctt cgagccgctg gcgctgacga ccggcgtacg tatggacgat
    13921 catgaaacct acggtgaaca ctggagtccg cgtgcctacc tggtttataa cgccaccgac
    13981 accgtaacgg tgaaaggggg ctgggcgacg gcatttaaag caccttctct gttgcaactt
    14041 agccctgact ggacgagcaa ttcctgccgt ggcgcatgta agattgtggg tagcccggat
    14101 ctgaaaccag aaaccagcga aagttgggag ctggggcttt actacatggg cgaagaaggc
    14161 tggctggaag gggttgaatc cagcgttacc gttttccgta acgatgtgaa agatcgtatc
    14221 agcatcagcc gtacgtctga cgtcaatgct gcaccaggct accaaaactt tgttggtttt
    14281 acagcgaacg gtgtgccggt atttagctac tacaacgtta acaaagcacg tattcagggc
    14341 gtggaaaccg agctgaaaat tccgttcaac gatgaatgga aactgtcact caactacacc
    14401 tacaacgatg gtcgtgatgt cagcaacggc gaaaataaac ctttatccga tctgccgttc
    14461 cataccgcta acggtacgct ggactggaaa ccgctggcgc tggaagactg gtcattctat
    14521 gtttccggac actataccgg gcagaaacgc gccgacagcg cgacggctaa aacaccgggc
    14581 ggttatacca tctggaatac cggtgcggcc tggcaggtga ctaaagacgt caaactgcgc
    14641 gcaggcgtgc tgaaccttgg cgacaaggat ctcagtcgtg acgactacag ctataacgaa
    14701 gacggacgtc gttactttat ggcagtggat tatcgcttct gatgagaaga tgcccggcga
    14761 accgggcgga ctttcacttc agtaaatact gcgcatggaa gcgcaggtga tcctctataa
    14821 aagaggcgat gaagtagtaa ctgtgatcat atcccggctg aatacgcagc gtcatcggcc
    14881 acgctttctg gcgggcggct tctgccagca ctgcgggttg cagctggtcg gcaagaaact
    14941 gatcattatc accctgatcg ataagcgtcg ggatcgcatc ctgcgcgtta ctggcataca
    15001 tcagcgcaca actgtcccat tccagccatg catttttgtc ctcacctaaa tagctgctaa
    15061 acgctttaat gccccagggt acgctgcatg gattcacaat cggcgcaaag gcggaaacgc
    15121 tggtgtattt gcccgggttt ttcagcgcca taatcagcgc accgtgacca cccattgaat
    15181 gaccgctaat agcgcagcgg tcgctgacat taaattgcga ctgaaccagc gccggtaatt
    15241 catcgcgcag ataatcatac atccggtaat gcgtcgccca cggcggttgc gtggcattaa
    15301 gataaaagcc tgcgccctgg cctaaatcgt agccatcgtc gttggcaacc tgttcgccgc
    15361 gcgggctggt gtctggcatc accagtacaa tccccagttc tgccgccacg cgctgggcac
    15421 ccgccttggt ggtgaagttc tcgtcattgc aggttaatcc ggaaagccag tacagcactg
    15481 gtggcggggt gtgatcacga ggtggtggga gaaagatact gaacgtcatc ggacagttta
    15541 aggtactgga gtcgtgtcgc cagcgttgct gccagccttc aaaacagcgg tgctcttcga
    15601 gcatttccat gcgtggctcc ttgttgtgtt gtttgcaatt tcttcatcat accgtttatt
    15661 caatgtgctg tgagtaactt tcacttccgt atttgcataa cgatgtttta acatctgctg
    15721 atgaaaggca gcggcaatta caataattat cgctgtgaat actggattat gtgcgccgcc
    15781 tcacgcacaa taatcaggct gtaaatcagc ttaataactt tgcccccacg cagggcggag
    15841 gcgtcacacc tgcaggagaa atcataaatg ccatcactca gtaaagaagc ggccctggtt
    15901 catgaagcgt tagttgcgcg aggactggaa acaccgctgc gcccgcccgt gcatgaaatg
    15961 gataacgaaa cgcgcaaaag ccttattgct ggtcatatga ccgaaatcat gcagctgctg
    16021 aatctcgacc tggctgatga cagtttgatg gaaacgccgc atcgcatcgc taaaatgtat
    16081 gtcgatgaaa ttttctccgg tctggattac gccaatttcc cgaaaatcac cctcattgaa
    16141 aacaaaatga aggtcgatga aatggtcacc gtgcgcgata tcaccctgac cagcacctgt
    16201 gaacaccatt ttgttaccat cgatggcaaa gcgacggtgg cttatatccc gaaagattcg
    16261 gtgatcggtc tgtcaaaaat taaccgcatc gtgcagttct ttgcccagcg cccacaggta
    16321 caggaacgtc tgacccagca aattctcatt gcgctacaaa cgctgctggg caccaataac
    16381 gtggctgtct ctatcgacgc ggtgcattac tgcgtgaaag cgcgtggcat ccgcgacgca
    16441 accagtgcca caacaacgac ctctcttggt ggattgttca aatccagtca gaatacgcgc
    16501 cacgaatttc tgcgcgctgt gcgtcatcac aactgattaa aaggcaggaa ccatggggcg
    16561 caacgtcacg ctcgattttg ttcgcggcgt cgccattctg gggatcctgc tattaaacat
    16621 cagcgccttt gggctaccaa aggcggctta tcttaatccc gcctggtacg gcgctattac
    16681 gccgcaggat gcatggacct gggcatttct cgatctcatc ggccaggtga aattcctcac
    16741 gctttttgcg ctgctgtttg gtgctggcct gcaaatgttg ctgccccgtg gcagacgctg
    16801 gatccagtcg cggttaacgc tgttagtctt gctgggcttt attcacggtt tattgttctg
    16861 ggacggcgat attctgctgg cttacgggct ggtgggctta atctgctggc ggctggtgcg
    16921 cgatgcgcca tcggtaaaaa gcctgtttaa taccggcgtc atgctttatc tggtggggct
    16981 tggcgttttg ctgttattgg ggttgatttc cgatagccag accagccgcg cctggacacc
    17041 tgatgcatcg gctattttgt atgaaaagta ctggaaactt cacggtggtg ttgaagcgat
    17101 cagtaatcgt gccgatggtg ttggcaacag tttactggca ctgggcgcac agtatggctg
    17161 gcaactggca gggatgatgc tcattggtgc ggcgctgatg cgcagcggct ggctgaaagg
    17221 gcagttcagc ttacgtcact atcgtcgtac tggttttgtg ctggtggcga ttggggtgac
    17281 cattaacctt cctgccatcg ccctgcaatg gcagctggac tgggcgtatc gctggtgtgc
    17341 ctttttactt cagatgccgc gggaactgag tgcgccgttt caggcgattt ttatggcttc
    17401 tggccgcaat tgagccgctt taagctggtg cttgcgatcg cctgcgtcgg acggatggcg
    17461 ctgaccaact atctattgca aacgctgatt tgtaccacgc ttttttacca cctcggtttg
    17521 tttatgcatt ttgaccgcct ggagctgctg gcgtttgtta ttccggtatg gctggcgaat
    17581 atcctcttct ctgttatctg gctgcgttac ttccgccagg ggccggtgga atggctctgg
    17641 cgtcagttaa ctttgcgtgc agctgggccg gcaagatcta aaacatcaag ataacgatct
    17701 ggatcacatt cgttaacaaa acggctgtaa ccgtttccat tgctgtgact cgattcacga
    17761 agtcctgtat tcagtgctga caaaatagcc gccagcaagc agtcatttac tgcaatctca
    17821 taacaggtag tgaatatgat caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg
    17881 gcaacggttt cccgggtgct caataacagc acgctggtca gtgccgacac gcgtgaagca
    17941 gtaatgaaag ccgtgagtga gctggattat cggccaaacg ccaatgccca ggcgctggca
    18001 actcaggtta gcgataccat tggcgtggtg gtgatggacg tttctgatgc gttttttggt
    18061 gcgctggtaa aagcggtgga tttggtcgcc cagcagcatc agaaatacgt gctaataggc
    18121 aatagctatc atgaagcgga aaaagagcgt cacgccattg aggtgttaat tcgccagcgg
    18181 tgtaatgcgt tgattgttca ctcaaaagca ttgagtgatg atgaactggc acaatttatg
    18241 gataacattc ctggtatggt gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc
    18301 gtttgcctgg ataatctcag cggtgcccga atggcgacgc gcatgttgct gaataacggt
    18361 catcaacgta ttggttatct ttcttccagt cacggcattg aagatgacgc catgcgtaaa
    18421 gcaggctgga tgagtgcgtt gaaagagcag gatattattc cgccggaaag ctggattggc
    18481 acaggtacgc cggacatgcc gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat
    18541 ctacaactta ccgctgtatt tgcttataac gacaatatgg ccgctggcgc actgacagca
    18601 ttaaaagata atggcattgc gattccgtta catctctcca tcatcggttt cgatgatatt
    18661 cccatcgccc gttacaccga cccgcaatta acgactgtgc gttatcccat tgcttcaatg
    18721 gctaaattag ccaccgaact ggccttgcag ggggcagcag gcaatattga tccgcgtgcc
    18781 agccactgtt ttatgccgac gttagtgcgt cgccattctg tcgcaacgcg ccagaatgcg
    18841 gcggcgatca ctaactcaac aaatctggcg atgtaaccgc tttcaatctg tgagtgattt
    18901 cacagtatct taacaatgtg atagctatga ttgcaccgtt ttaacgttgt aacccgtatg
    18961 taacagtgaa taatcacttt caccgaggta atagcgtcat aacaacaatt aaagccgttt
    19021 tctggagcgt taccgggcat ggaagaacga attttaaaaa gtgagcttcg gcgttcagta
    19081 acacttcatt aactctactg ccccgccgag catttatctc aagcactacc ctgcataaga
    19141 aaaaccggag ataccatgaa taagaaggtg ttaaccctgt ttgctgtgat ggccagcatg
    19201 ttattcggtg ccgctgcaca cgctgctgat actcgcattg gtgtaacaat ctataagtac
    19261 gacgataact ttatgtctgt ggtgcgcaag gctattgagc aagatgcgaa agccgcgcca
    19321 gatgttcagc tgctgatgaa tgattctcag aatgaccagt ccaagcagaa cgatcagatc
    19381 gacgtattgc tggcgaaagg ggtgaaggca ctggcaatca acctggttga cccggcagct
    19441 gcgggtacgg tgattgagaa agcgcgtggg caaaacgtgc cggtggtttt cttcaacaaa
    19501 gaaccgtctc gtaaggcgct ggatagctac gacaaagcct actacgttgg cactgactcc
    19561 aaagagtccg gcattattca gggcgatttg attgctaaac actgggcggc gaatcagggt
    19621 tgggatctga acaaagacgg tcagattcag ttcgtactgc tgaaaggtga gccgggccat
    19681 ccggatgcag aagcacgtac cacttacgtg attaaagaat tgaacgacaa aggcatcaaa
    19741 actgaacagt tacagttaga taccgctatg tgggataccg ctcaggcgaa agataagatg
    19801 gacgcctggc tgtctggccc gaacgccaac aaaatcgaag tggttatcgc caacaacgat
    19861 gcgatggcaa tgggcgcggt agaagcactg aaagcacaca acaagtccag cattccggtg
    19921 tttggcgtcg atgcgctgcc agaagcgctg gcgctggtga aatccggtgc actggcgggc
    19981 accgtactga acgatgctaa caaccaggcg aaagcgacct ttgatctggc gaaaaacctg
    20041 gccgatggca aaggtgcggc tgatggcacc aactggaaaa tcgacaacaa agtggtccgc
    20101 gtaccttatg ttggcgtaga taaagacaac ctggctgaat tcagcaagaa ataagactga
    20161 ttgggcgcag tcgtttactg cgcccttatt gtatggccaa caaggtaaaa ttatatggtc
    20221 agctcaacga ctccgtcttc cggggaatac ttgttggaaa tgagcggtat caacaagtct
    20281 tttccaggtg ttaaggcact tgataacgtt aatttaaaag tccggccaca ttctatccat
    20341 gcattaatgg gggaaaacgg cgcaggaaaa tcaacattat taaaatgcct gtttggtatt
    20401 tatcaaaaag actccggcac cattttattc cagggtaaag agatcgattt ccattctgcc
    20461 aaagaagcgc tggaaaatgg tatttcgatg gtacaccagg agttaaacct ggtattacaa
    20521 cgttcggtga tggacaacat gtggctgggg cgatatccca ccaaaggcat gtttgtcgat
    20581 caggacaaaa tgtaccgcga aaccaaagcg atttttgatg aactggatat tgatatcgat
    20641 ccgcgtgcgc gcgtcggcac attatccgtt tcgcaaatgc agatgatcga aatcgccaaa
    20701 gcgttttcct ataacgcgaa aattgtgatt atggatgaac cgacttcttc gttaaccgaa
    20761 aaagaggtca atcatctgtt cactattatt cgtaaattaa aagagcgcgg ctgcggtatt
    20821 gtttatatct cacataaaat ggaagaaatc ttccagttat gtgatgaagt taccgtattg
    20881 cgcgacggtc agtggatcgc cactgaaccg ctggcaggac tgacgatgga caagatcatc
    20941 gccatgatgg ttgggcgttc tcttaaccag cgtttccctg acaaagaaaa caagccgggc
    21001 gaagtgattc ttgaagtgcg taacctgacg tcgctgcgcc agccgtcgat tcgcgatgtc
    21061 tcgtttgatc tgcataaagg ggagattctc ggtatagccg gtctggtggg agcgaaacgt
    21121 accgatattg ttgagacgtt atttggtatt cgcgagaaat cggctggcac cattacgttg
    21181 cacggcaaaa agatcaataa ccataatgcc aacgaagcca taaaccacgg atttgcactg
    21241 gtaactgaag agcgccgctc aacgggaatt tatgcctatc tggatatcgg ttttaactcg
    21301 ttaatttcca atattcgcaa ctacaaaaat aaagttggtt tactggataa ctcgcggatg
    21361 aaaagcgata cccagtgggt gattgattcg atgcgggtaa aaacaccggg gcatcggacg
    21421 caaattggtt cgctctccgg cggtaatcag caaaaggtga ttattggtcg ctggctacta
    21481 acgcaaccag aaatattaat gctcgatgaa ccgacgcgcg gtattgatgt cggagcgaag
    21541 tttgagatct atcagttaat tgccgaactg gcgaagaaag gcaaagggat tattattatc
    21601 tcctctgaaa tgcctgagtt gttagggata acagaccgta ttctggtcat gagcaatggt
    21661 ctcgtttccg gaattgtcga tacaaaaaca acaacgcaaa acgaaattct gcgtcttgcg
    21721 tctttgcacc tttaagatta ggggcttccc atgagtgcgt taaataagaa aagttttctt
    21781 acttacctga aagagggcgg tatttacgtc gttcttttag ttttgctggc aattattatt
    21841 ttccaggacc caacattttt aagtctgttg aacttaagta atattctcac ccagtcatcg
    21901 gtgcgtatta ttatcgcgct cggtgtggca gggttaattg tcacccaggg gaccgacctt
    21961 tctgcaggtc gccaggtggg gctggcggca gtagtagcgg cgacgttatt gcagtccatg
    22021 gataacgcca acaaagtgtt cccggaaatg gcgacgatgc cgattgcgct ggttattctg
    22081 attgtctgtg ccattggtgc ggtgatcggt ttgatcaacg gtctgattat cgcctatctc
    22141 aacgtgacgc cgttcattac cacgctcggc acgatgatca tcgtctatgg catcaactcg
    22201 ctctattacg actttgtcgg ggcgtcgccg atttctggtt tcgacagtgg cttctctacc
    22261 tttgctcagg gctttgtcgc gctggggagt ttccgtctct cttacatcac cttctacgcg
    22321 ttgattgcgg tggcgttcgt ctgggtgttg tggaacaaaa cccgcttcgg taagaacatt
    22381 tttgccattg gcggtaaccc ggaagcggcg aaagtatctg gtgtcaacgt cggcctgaac
    22441 ctgctgatga tctacgcgtt gtctggcgtg ttctatgcct tcggcgggat gttagaagcc
    22501 ggacgtatcg gctctgccac caacaacctc ggctttatgt atgagttgga tgctatcgcg
    22561 gcatgtgtgg tgggtggtgt atcgttcagc ggtggtgtag ggacggtgat tggcgtggtg
    22621 accggggtga ttatttttac cgtcatcaac tatggcctga cgtacatcgg cgtaaaccca
    22681 tactggcagt acatcatcaa aggggcgatt attatcttcg ccgtggcgct ggattcactg
    22741 aaatacgcgc gtaagaaatg atgtgaatgt gggtattgcc ggatgcgccg catccggcaa
    22801 tcggtgccaa atgccggatg cgtctctggt tttacaacgt taccgggtgt tctttctctc
    22861 ccttcttaaa cttcacttct ccgagatcaa tacaacccac cgggcagacg tgaccacaaa
    22921 gcaggcaacc aacacatttc tcggtattac aatgcggtgt acgggttttc tcgctccatt
    22981 ccatcgcctg gtgaccgccg tcgtaacagg aaatataaca gcgtccacag ccaacacatt
    23041 tatcgagatt gatatgggga tagacaatat aactgcggtc taaatcttcc gccgggacaa
    23101 tattgttatt cgccagacct accatctcct gcaacgaatc aaacccttga tcggcgagat
    23161 aatgcgacaa cccgcttgcc atatcttcca ctatccgata cccgtactgc ataatgccgg
    23221 tggtcacctg caacgtcgct gcaccgagca ataaaaactc cgccgcatct tcccaggttt
    23281 caatgccgcc gataccgctg atcgggaaat cgcgcagttc tggatgggta cgcatttgct
    23341 gaatgaagcg cagggcgatc ggttttaccg ctttaccgga atatccggaa atactcgatt
    23401 taccgttaac gatcggcata ccgattttct gattaagatc gatattggtg atggatttaa
    23461 cggtgttaat cgccgcaatg ccatctgcgc cgccgcgctt cgccgccagc gccacttcgc
    23521 acatatcacc gatattcggc gtcatcttcg ccagcattgg cagcgtggaa ccacgtttta
    23581 ctgcccgaca atatttttct accagctccg ggctttgccc gacatcgcta cccatcgcat
    23641 gagaagtcat ttgcggacag gagaagttac actcgatcat atccgcgcca gcttcttgca
    23701 ccagacgcgc cagctcttcc cattgctgct cattttcgcc catgattgaa gcgatcaata
    23761 ctttgtccgg gtaatcttct ttcagccgac gcagtgcggc cagattatct tccaacggat
    23821 gttcagcaat ctgctccata tttttgaagc cgataaaacc ggtatcttct ttcaccagat
    23881 gatcaaaacg cggcgagact tcgttggcga taaaaaaacc gatcgtttta aacaccacgc
    23941 caccccagcc tgtgtcgtag gctttggcac acatctcata gcagttgcct acaggcgaag
    24001 aagagaggca gaacgggttg ggaaacttca caccacaaaa ggtaatcgaa agatctttcg
    24061 ttaacatgag caagctccct ttaaatagtg atgaatcgcc tcagcggctt ctttcccggt
    24121 tttcacggca taaaccacgg ttttgtcacc ctcaacaata tcgccagcag caaagacttg
    24181 cgggtcgcgg gtctggtaat tttgtgtttt gatggtgtta cgctgcggct ctaactccgc
    24241 aaaggcatcc agtctggcat gctgaccgac ggcgagaata attttatctg ccgccatcgt
    24301 cagttcgccc ggtaaccgta catgcttaaa cgtgacttta ttgccttcca cagctactgg
    24361 cgtgaatcca tcgatgatcg aaacgcccag ctcccgggcg ctggcaaact ctttttcgct
    24421 tgccggaaac tcatctaact cttcacgcgc tacgcaagtt actgcctgac agccgagaac
    24481 tttcagcgtg ctggctacgt ccatcgcgac atcaccgccg ccgataatta atgcgctttg
    24541 tggaatgctg atatcgcctt gcgcctgtcg tgcacgttgc aagaagtcga cggcaatctc
    24601 aacatcgcta tgctcaaaca gtggtagccc cgaaccgctc gataacccca cagtgaccag
    24661 taccgcgcgg ttttccgctt tcagctgctc cagggtgagt gtgttaccga tttcgttatt
    24721 gcatttaatg gtcacgccca ttttttcaat acgggcgatt tctgcatcca gtactgattg
    24781 cggtaaccgg aattgcggaa tgccgttacg caaccagcca ccgggttgtg cttctttctc
    24841 atagatcgtg acgtcataac cctggtttgt cagtgtcaca ctggcctgca atccggcagg
    24901 acccgcgcca ataatggcga ctttgccgag cgttttagta ccgggctgat aaatttccat
    24961 cccagtttgt tgttcaaaat cagtaacaaa acgctgtaag cgaccgatat caatgggagt
    25021 atcaacaccg gcacgggtac aaccgctttg gcataatttc tccgtcgggc acactctggc
    25081 acaaacggca ccgagggcat tattttcacg aattgtctcg gcagcgcctt taaaattacg
    25141 aaagtagatt gagcgaataa atttccccgg atcggtctgt gccgggcaag cctgactaca
    25201 gggggcgtca tggcataata aacagcgaga ggcttcttta atcgccagta acggtgtaaa
    25261 agccggagtg agttcatcca gataattttg ttgcggcatg tgatcttcct taatgctatg
    25321 agtaattaag gcgaacgcta aatatactcg tcatacttca agttgcatgt gctgcgtctg
    25381 cgttcgctca ccccagtcac ttacttatgt aagctcctgg ggactcactc acttgtcgcc
    25441 ttcctgcaac tcgaattatt tagagtataa atattgcgtg ctgatattaa ggctatgcgc
    25501 gtggggaaat ttaaactgtg aaaggattca caaatagctg cggttcagaa tgttatatcc
    25561 gcagcttatt gagtgaagta ataattattc tcaataatta acagaaaaga gaaaatatta
    25621 acgatagaaa ataagataaa caatcatgcc gatagataaa attccgaagc cgatatatgc
    25681 caccatataa gcgaagaaga gtccgggatt ttttgctcgc cagacataca ccggttccgg
    25741 tgcattttta acccgtttat caatcgcgcc tgcgcgccag cctttccatg cttcgcggaa
    25801 acaaaatacc gggataagga aaaaaacggc aacgacaaaa aactgctgta catccataac
    25861 tactttcctt gtttcttttg taattcaagt aactgttcgg gtgtgacggc gggatacccc
    25921 tgcgcatctt caggcacctg gtgcatagcc ggaataggga ccagcggccc taaaaaacgc
    25981 ggttcacgtt taaaaatata aaggtcagcc agcgcaccga aacgggcggc aaattcgcga
    26041 atacgtactg acagcatatc tttcggtgac ggtacggcat aacactgagc ctgaatcccc
    26101 atatgcagcg caataaataa tgcccgctca cagtggaaac gttgggtgat aataatgaaa
    26161 tcattagtat cgaaaacttt gcgtgtacgc acgatggaat ccagcgtacg aaagcctgcg
    26221 taatcgagaa caatatctga tgggtcgaca ccagcagcga ttaaatcttt gcgcatggtc
    26281 atcggctcat tataactttg caatgcgtta tcgccgctca gtaatagata atttacctta
    26341 ccgctgttat aggcattaat cgctccttga atgcggtagc gataatactg attaattacg
    26401 ccagtacgat aatattttgc tgttccgagc accacaccga cctggcggta ggggagatcc
    26461 tgcaattcgt cgtagatata aggcgcggtt ttccagctca tccagcgatc gaggccgagc
    26521 acagtcaaca gcagcaagcc gatcaggact aacaggctga ggaacacgcg ctttaacatg
    26581 caggagactc gatctaagga aaatttccat caggctactt tacccgctgg ttttgcgcaa
    26641 gaaacggcaa actgtttgcc gggattttcg acaccaggag agggctggca agccggacaa
    26701 ggcgttcacg ccgcatccgg caccaggctt aagcgagaag cacgcggtcg atattgtgac
    26761 agccgagagc tttcagtgtt gcggaggtgg catcccactg aatcaacggc gcatcggctt
    26821 tttctgccag aaccgcgcgc tggatatccg ggtaatcata acccttgaga ttcaacagaa
    26881 ttaacgcccc ctgcaacggt ggcagagtcg ggttgaatgc ggcgttttca gcgtagctgc
    26941 cgctgaaaat acgaccgtct ttacattcca gcgcgacacc gcttggcgac ttactgtaag
    27001 gcatgtgcga acggtttgcc gccgcaatcg ctgcctgaga aagcgcatca cccgtcagcg
    27061 catagccgtg atcttgctcg tccatcagca gcgttttaat ctccagatct ttcggcccaa
    27121 aggcatccgg cagatagtca cgcagcgcgt gtgcctcgcg gcccggcaga tgaatacgca
    27181 gatccagacc gctgttcagt tcattcataa actgacggca gtgaccacaa ggcgtatagt
    27241 taacggtgat ggctacaagc gctttttcac cactcaacca ggcgtggctg atcgcgcttt
    27301 gttcggcatg aacggtttgc tgcattgtcg caccaataaa ttccatattg gcaccaaaat
    27361 accaggttcc gctcacaccg cgcgcaattg cgccaacatt aaaattcgac aatggcgtac
    27421 gcgcacaggc tgccgccagc ggaagtagtg cgaatgccag cgcgtcttcg tccagccccg
    27481 ttgcgctctt cagcgatgag acttgctccc cggtcaacaa agcggggaag tacttgtctg
    27541 ccagaatagg ttccagtgca gattgcaagt tatccgcaag ttgggcaaaa gcggtttgaa
    27601 aacgtggatg catggcgttg cctcataatc atgtaatggg ataacagttt acgaacccgt
    27661 tatggcttta tatgtgatct gaatctcatt aattatgcaa cgcgtgaaac ttacatcaaa
    27721 aatgcgcgac gcatcgcaaa ttttagccca ttactgccag aataatcggg aaaaggaacg
    27781 gtgcgatcag cgaagtaata atcccgcata acaccagcgc cagcgaacta aatgcacctt
    27841 cctgataatc cagctcggcg cagcgcgccg taccgagggc gtgcgaggct gtgcccattg
    27901 ccagaccgcg cgcagctttg gtacgaatac gcatcgcatt aagcaatgta tggccaaaca
    27961 cagcgccgag gatgccgacg aaaatcacgc aaactgcgct gattgccgga ataccgccaa
    28021 tactgccgcc aaccgccatt gcaataggcg tggtgactga tttcggcagg attgacgcgg
    28081 cgatttccgg tgaagcgccc atcaacaatg ccacagaagt ccccgtcacc atcgcaacca
    28141 cgctgccgat aaaacagatg gtgatgatcg atttccagcg cgcgcggatc tggtgcagct
    28201 gctcatataa aggataggcc agtgcgacaa ccgccggttg aagcagatcg tttagcactt
    28261 cgctaccttt aaagtagctg tcgtaagaga tgccggtcag cattaaaaaa ggaatgatca
    28321 ccaccattgc taccagcaac gggttaagca ggggaaactt ataccgtgcc gccagtttgc
    28381 gggcggcaaa aaagacaatc aacgtcagcg gtaatgacca ccagatattc gccatcattc
    28441 ttctgatcct ttctgaccta cgactttacg ttcaccgtgc accagttgtg aactccagct
    28501 caccaccaga aaaaccacca gcgtactgac tgcacaggaa accactaccg gaccaaactg
    28561 tgcgcggagc aaatcaaaat attgcatgac gcctacacca atcggcacaa acaatagcgc
    28621 catatagcga atcagtacgt agcaccccgg attgacccat ttcgccggaa gaatttgcaa
    28681 ggccagcagg acaaacagga tcagcatccc gatgatgctg cccggaatgg ttaccggtag
    28741 cagggaagca atgaaaatgc ctgcatacag gcaggcataa atcaggacaa aagcacgtaa
    28801 atattgccag ataatgttca gtgtcttact cataatcaac accccagatg agatggattc
    28861 atcatacaat taattcgtaa aacgtgctac cgatcacatt aagaaatctc agcatagttg
    28921 aattttaggg acgtaacctc gggcgagcgc ccagttcagc acactaattc tgctgtaaaa
    28981 agtgcagaaa ctgacttaaa gcataagaag gcgtttcgtt attgcgccag aacacaccga
    29041 cgctgccttt ctgttctatc tttggcaggt tgagaatcac cagttgtccc tggctggcat
    29101 aacgttgcgc caggcgtaat gacaaaatag agatcatatc gctgctttgt aacaggttgg
    29161 tactgacgtt catcgacgcc gactcaatcg tgttttctgg cagcatcacg ccgtggtcta
    29221 ccagcgcatt atcaatgctg atgcggatgg gggtacctgt cggccagact atccaccgcc
    29281 agttagcaag atcattccag ctgagggtgg cgtatttcgc cagtggatgc tcaggacggg
    29341 cgacaaaaca caccggctcg gtgtagagca cgcgataatt cagcggcagc tgcaacgcgc
    29401 gaccgccaat tcgcccaacc accacatcga cagaacctgc caacagatct tgcagcaatg
    29461 gggtcatcac cttctcttcg atattcaggt gtagagttgg catctccttg agcaaatgca
    29521 aaatcgcctg ggatacgcag tcagtggcga cgggagaaca gccaattttc agactgccga
    29581 ccagtccacc ttgtttaaaa cgggccagtt catgttgcga gcgctccatg tcattgatta
    29641 accgttgggc atgttgcaat aacaatttgc cgccttcagt aggacgaagt cctttgctgt
    29701 gccgctcaaa gagtgtgagg ccgatctcat cttcgagttg tgacaaccat ttcgacagcg
    29761 caggctgggt gatattcatc atccgcgcga catgggtcag attgccctgt tcacccagcg
    29821 ccaccagcat ttgcaggtga tgaagcttta gtttctgcgc ccagtttgcc atttgccata
    29881 acctccaggt tatctctctg tccgaaatgc cattgtacat ttcatctctt taatcacaat
    29941 atcgcaacat acataaaaca aatgaaacat ctaccttacc tgcactgagg catttactat
    30001 gactcaacga cgtgatttac aagcactgat cgatgctgct ccggtcggca agatgcagtg
    30061 gcgcgttatc atctgctgtt ttctggtggt aatgctcgat ggttttgaca ccgcggctat
    30121 cggctttatc gcccctgata ttcgcactca ctggcaactc accgcaggcg atctcgcccc
    30181 gctttttggc gcgggtctgc tggggttaac cgcaggcgca ctgctttgtg gtccgttgtc
    30241 cgatcgcttt ggccgcaaac gggtgattga gctgtgcgtc tttttgtttg gcgcgttaag
    30301 cctcgcctcg gctttctcac cggatctgca aacgctggta ttcctgcgtt tcctcaccgg
    30361 ccttgggctg ggtggcgcga tgccaaatac aattaccatg acctcggaat atctgcctgc
    30421 ccgccgccgc ggcgcgctgg tgacgctgat gttttgcggc tttaccctgg gttcggcgtt
    30481 tggcgggatt gtcagcgcgc agctggtgcc ggtgattggc tggcacggga tcctggtgtt
    30541 gggtggcgta ttaccgctga tgctgtttgt tgcactgctg gtggttctgc cggaatcgcc
    30601 acgctggcag gtgcgccgcc agctacccca ggcggtgatt gcgaaaaccg tcagcgccat
    30661 catccgcgag cgttatgtcg atacccactt ttatctcatt gaatcagcct ccgttacaaa
    30721 aggcagcatt cgtcaactgt ttatggggcg tcagttaccc atcacgttga tgctgtgggt
    30781 ggtgttcttt atgagcttgc tgattattta cctgctctca agctggatgc caacgctgct
    30841 caaccatcgc ggcatcgacc tgcaacatgc ctcctgggtt accgccgcgt tccagattgg
    30901 cggtacgttg ggcgcgctgg cgctgggcgt gcttatggat aagttcaatc ctttccgcgt
    30961 actggcgctg agctatgcca taggcgctgt atgcatcgtg atgattggct taagccagga
    31021 cggattatgg ctaatggcgc tggcgatttt cggcaccggc attggcatca gcggatcgca
    31081 ggtggggctg aatgcgctga ccgcaacact ttatccaaca caaagtcgcg ccacgggcgt
    31141 gagctggtca aacgccgtgg gccgctgcgg cgcgattgtt ggctcgctgt ctggcggcgt
    31201 tatgatggcg atgaatttct ctttcgacac cctgtttttc attatcgctg tgcccgcggc
    31261 gatcagtgcg gtgatgttaa cccagctgat caccgtggtt cgccagtcga cctctgtccc
    31321 tgactcactg cctcacgcag gtgttgtgaa cgaataagga gcatcccaat gactgataac
    31381 aatcaaaata gccgcgagca attttatcag catatttccg ggcaaaacct gaccccgctg
    31441 tgggagtcac tgcaccatct ggtgccaaaa acgcccaacg ctaactgtgc gccagcgtac
    31501 tggaattatc aggagatccg cccgttgctg ctggagagcg gcagcctgat tggcgcgaaa
    31561 gaagccgtgc gccgcgtgct ggtgctggaa aacccggcgc tgcgcgggca gtcttccatc
    31621 accgcgactt tatatgccgg tttgcaactg atcatgccgg gcgaagtggc accgagtcat
    31681 cgccataacc agtcggcgct gcgttttatt gttgaaggta aaggggcatt caccgccgtt
    31741 gatggcgaac gcacgtcaat gaatgagggc gattttatcc tgaccccgca gtggcgctgg
    31801 cacgatcacg gtaaccctgg cgacgaaccg gttatctggc tcgatgggct ggatctgcca
    31861 ttagtgaaca ctctgggctg cggttttgcc gaagattacc cggaggagca acaaccagta
    31921 acgcgtaaag agggcgatta cctgccgcgt tacgctgcca atatgttgcc gttgcgccat
    31981 cagaccggga actcctcgcc catctttaac taccgttatg accgtagccg cgaagtgcta
    32041 cacgatttaa cccgcctggg cgatgccgat gagtgggatg gctacaaaat gcgctacgtc
    32101 aacccggtca ccggtggcta tccgatgccg tcgatgggcg cttttctgca actgctgccg
    32161 aaagggtttg cctcacgcgt ggcgcgtacc actgacagca ccatctacca tgtggtggaa
    32221 ggtagcgggc aggtcactat cggcaatgaa accttcagct ttagtgcaaa agatatcttc
    32281 gtggtgccga cctggcacgg cgtgtcgttc cagaccacgc aagataccgt tttatttagt
    32341 ttttcggaca gaccggtaca agaagccctg gggctgttcc gcgaagcacg ttattaattt
    32401 gggagagaga aaatgactca atacgtattc gaaccacaag cgcccgttac cgtaccggtt
    32461 gttggcagtg atgaacaatt ccccgtacgc cgcgtttact gcgtcggacg taactatgcc
    32521 gcccacgccc gcgaaatggg ctttgatccg gatcgtgaac cgccattctt cttctgcaaa
    32581 ccggcggatg ccattgttcc ggttgccgct ggcgaaacgc tggcgctgcc gtatccggcg
    32641 cagaccgata actaccatta tgaaattgaa ctggtggtgg cgattggtaa aaaaggcagt
    32701 gatatcccgc tggaaaacgc ccatgaatat gtctggggtt atgccacggg tcttgatatg
    32761 actcgccgtg accgtcaaat ggaaatgcgc cagatgggcc gcccgtggga aatcggcaaa
    32821 gcgttcgatc tctcggcccc catcgcgccg ctgcacaaag ccagcgaaac tggcggtatt
    32881 gataacgcac ctatctggtt acaggttaac ggcgaagatc atcagcgcag cgatattcgt
    32941 catctcatct ggtcagtgaa tgaaaccatc agctacctgt caggcttctt tgaactgcaa
    33001 ccgggcgatc taatctttac cggtacgccg gaaggggttg gctcagtagt caaaggtgat
    33061 gtgatcacgg gcaacgtcga gggcttaacg cctatcgcgg tgaagattgt ctgaggtgat
    33121 gatgaagctg tacagttttt ttaatagttc agcgtcttac cgggtgcgca ttgcgctggc
    33181 gctgaagggt atcgattacc agaccgaagg ggtgaatatc cgcattggtc agcaaaacga
    33241 actggcctac cggcggatga atccggtcgg gctggtgccg acgctgctga ccgacgaggg
    33301 cgaatcactc ggacaatcgc tggcgattat tgactggctg gagagtcact acccgcaagt
    33361 tccgctgatg ccgcaggaag aaccggcccg caacaaggtg ctggaaattg tctatgccat
    33421 cgcctgcgat attcatccat tgaataacct acgcgtgctg cgctacctga ccgaagagct
    33481 gaacgtcagt gaagaagata aaaaacgctg gtatgcgcac tggatccagc aagggttaag
    33541 cgcggtggaa cagctactgc gccagagcca gtcggggcaa ttttgtgtgg gcgagacgcc
    33601 gacgctggca gattgctgcc ttgttccaca gtgggcgaac gcgctacgta tgaactgcga
    33661 tctgagcggg tatccgcgct gcaaagcggt atacgacgcc tgcacgcagc tgccagcgtt
    33721 cattgccgca gcgccagaaa atcaacaaga taaaatttca gcctgaccag gagaaccata
    33781 atgactaaag tgacacgcgc agtaattgtg ggaggcggga tcggcggtgc ggcaactgca
    33841 ttgtcactgg cccgccaggg gatcaaagtg gtgctgctgg aaaaggcgca tgagatcggt
    33901 gagattggcg caggtatcca gttggggcca aatgcgtttt cggcgttgga tagcctcggc
    33961 gtcggcgaaa tcgcccgcca gcgcgcggta ttcaccgatc acatcaccat gatggatgcg
    34021 gtaaacgccg aagaagtggt gcgcattgaa accggtcagg cctttcgcga tcatttcggc
    34081 ggcccttacg cggtgatcca ccgggttgat attcacgcct cggtgtggga agcggtactg
    34141 acgcatccgg acgtggaata tcacacctcc accaacgtcg tggatattcg cgagacagaa
    34201 gacgatgtca ccgttttcga cgatcgcggc aatagttgga tcggcgatat tctgattggc
    34261 tgtgacggcg tgaagtcagt ggtgcgccag agcttattgg gcgatgcgcc gcgtgtcacc
    34321 gggcatgtgg tctaccgtgc tgttatcgat tgtgccgata tgccggaaga tttgcgcatt
    34381 aacgcaccag tactgtgggc ggggccgtgc tgtcacttgg tgcattaccc tttacgcggc
    34441 ggcaagcagt ataacctggt ggtgacattc cacagccgcc agcaggaaga gtggggcgtg
    34501 aaggatggca gtaaagagga agtgctctct tactttgaag ggattcaccc ccgtccgcgc
    34561 cagatgctgg ataaaccgac ctcctggcga cgctggtcaa ccgccgaccg tgaaccggta
    34621 gcgaagtggg gaacgaagcg cattacgctg gtgggcgatg cggcccatcc ggtggcgcag
    34681 tatatggcgc agggtgcctg tatggcgctg gaagacgcgg taacactggg taaggcgctg
    34741 gcgcagtgtg agggtgatgc ggcacaagcc tttgcgctgt atgagtcggt acgtattccg
    34801 cgcaccgcgc gcatcgtctg gtcgacccgt gaaatggggc gggtttatca tgccgcagga
    34861 gtagagcgcc aggtacgtaa cctgttgtgg aaagggaaat cgcaggcaga gttttaccgc
    34921 ggcatggagt ggctgtacgg ctggaaagaa gataactgtt tgcaaccacg ctgagtactt
    34981 taatccgctg accataggcg ctaacatagc gcctcatttt tttgcgggtg atgatatgcg
    35041 tgtgttactg gcaccgatgg agggtgtact cgactctctg gtgcgtgaat tgctgaccga
    35101 agttaacgac tacgatctgt gcatcaccga gtttgtccgc gtggtggatc aactgctgcc
    35161 ggtaaaagtc tttcatcgca tttgccctga gctacaaaac gccagccgga caccagccgg
    35221 tacgctggtg cgcgtgcagt tgttaggtca gttcccacaa tggctggcag agaatgccgc
    35281 ccgtgcggtc gagttaggtt cctggggcgt ggatctcaat tgcggctgcc cgtcgaaaac
    35341 ggttaacggt agcggcggtg gggcgacgtt actcaaagat cctgaactca tctatcaggg
    35401 tgcaaaagcg atgcgcgaag ctgtaccggc gcatttgcct gtcagcgtaa aagtgcgtct
    35461 gggctgggac agcggtgaga agaaatttga aatcgccgat gcggtgcaac aggctggcgc
    35521 tacggagctg gtagtacatg ggcggacaaa agagcagggt taccgcgcgg agcatattga
    35581 ctggcaggcg attggcgaga ttcgccagcg gttgaatatt cctgtgattg ccaacggtga
    35641 aatctgggac tggcagagtg cgcaacaatg catgacgatc agcggatgcg acgcagtgat
    35701 gattggtcgc ggggcgctca atattcccaa cctgagccgg gtggtaaaat ataacgaacc
    35761 gcgaatgccg tggccggagg tagttgcttt gctgcaaaaa tatacccgtc tggaaaagca
    35821 gggcgatacc gggttatatc acgttgcgcg gattaaacag tggttgagtt atttgcgtaa
    35881 agaatacgat gaagcaacgg aattatttca gcatgttcgg gtgttgaata attcccctga
    35941 tattgcaagg gctattcagg caattgatat cgagaaactc taagaaacca tagtgatgtt
    36001 ttttgacaat gtgttagctc tttatcaata ggccgcacgg ttgtttggtg gcgtcaaaat
    36061 cgccgaggcg ttccctgaag gccggggcag cccacatgga tgtgggctga gggcgcgttt
    36121 tacagggatg ttacctcgcg cccgacccgg tagccgtaag ggataagccg agggcaccgc
    36181 gcagcggcga ttttgttcgc cagagcccgg gggtgcaggg ggcggcggcg attggccgcc
    36241 ccctgcgcgc tccttgcgcc agtgattata tgaagcaatg cacatgacag gagcgcaaca
    36301 ggatgagaga ctgaaatatt ctcgttattt tcggatctga tgacattaat taattttaaa
    36361 atatcatctt aaatacgcca gtcaccacca gtagcccaat caggaaaata atcaatacag
    36421 cccagagtat aattttcatt ggcgttcctt tatttgtgag tgctgaataa gtataggaaa
    36481 aaacgaagcc ctttgccgct atccataaac gattaagata aatcggaaga tatcattcac
    36541 ttggtgtata tttttctgga tatgtcgttt tattgccact gatgtattgc cacgcgaaac
    36601 taaaaaatat atcgcaacac acggtaatct ccgcgcacct tttcttacct gattattccc
    36661 ccatgaatcg tgattccttt tatccagcca tcgcctgttt tccgctgtta ctgatgctgg
    36721 ccgggtgtgc gcctatgcat gaaacccgcc aggcgttaag ccagcacacg cccgctgtgc
    36781 aagttgacac cgcattaccc acggcgctga aaaatggttg gccagacagc caatggtggc
    36841 tggagtatca cgataatcaa ctcacctcat taattaataa cgcactacag aacgcaccgg
    36901 ttatgcaggt ggctgaacag cgcattcagc tggctgaagc ccaggcgaag gcaattgcta
    36961 ctcaggatgg tccgcagctc gacttttctg cggatatgga gcggcaaaaa atgtcggcag
    37021 aaggcttaat ggggccgttt gctctgaacg atccggccgc aggtacgacc ggtccgtggt
    37081 acaccaacgg tacttttggc ttaacggcgg gctggcatct cgatatctgg ggaaagaatc
    37141 gggcggaggt aactgctcgc ctgggtacgg ttaaagcacg agcggcggaa cgcgagcaaa
    37201 cccgccaatt gctggctggc agcgtagccc gcctgtactg ggagtggcaa acccaggcgg
    37261 cgttaaacac ggtcttgcag caaatcgaaa aagagcagaa caccattatc gccaccgatc
    37321 gccagctata tcagaacggg attacttctt cagttgaagg tgtggaaacc gatattaatg
    37381 ccagcaaaac ccggcagcag ctcaacgatg tcgcggggaa aatgaaaatt attgaggcac
    37441 ggttaagtgc acttacaaat caccagacaa aggcattgaa gcttaatccg gtcgcgttgc
    37501 cgaaagtggc aagccagctt cctgatgaac tggggtactc cttactggcc cggcgggcag
    37561 atttgcaggc ggcgcactgg tacgttgagt catcgttaag cactattgat gcggcaaaag
    37621 cggcatttta tcctgacatc aacctgatgg ccttcctgca acaggatgcg ttgcacttaa
    37681 gcgatctgtt ccgtcattcc gcgcagcaaa tgggcgttac ggcaggcctg acgctaccca
    37741 ttttcgatag tggtcgtctt aacgccaacc tcgatatcgc aaaagccgaa agcaacttgt
    37801 ctatcgccag ctacaacaaa gcggtggttg acgcggtgaa tgacgtggcg cgggcagcca
    37861 gtcaggttca gacactggcg gagaaaaacc agcatcaggc gcaaattgag cgcgatgcct
    37921 tgcgtgtagt aggtcttgca caggcgcgct ttaacgcggg catcattgcc ggttcccgcg
    37981 tcagcgaagc cagaatcccg gcgcttcgcg agcgggccaa tggcctgtta ttgcaagggc
    38041 agtggctgga tgcctccatt caactcactg gtgcgttggg cgggggttac aaacgctgat
    38101 gatatgcgcc ttctatactt aacgtttatt cagcgttaag tggagaactc gatggcacag
    38161 gttgcgatta ttaccgcctc cgattcgggg atcggcaaag agtgcgcgtt attactggcg
    38221 cagcaggggt ttgatattgg cattacctgg cactctgatg aagaaggggc aaaagatact
    38281 gcgcgtgagg tggttagcca cggcgcacgt gcggagatcg tgcagctgga tctcggtaaa
    38341 ctgccagaag gtgcgcaggc gctggagaaa ctcattcaac gactggggcg catcgatgtg
    38401 ctggtgaata atgcgggtgc aatgaccaaa gcgccgtttc ttgatatggc ttttgatgag
    38461 tggcgcaaga tttttaccgt tgatgtcgac ggtgcattct tatgctcgca aattgcggct
    38521 cgtcagatgg tgaaacaagg gcagggcggt cgcatcatca acattacgtc ggtgcatgaa
    38581 catacgccgc tgccggatgc cagcgcttac acagcagcta aacatgcgct cggcgggtta
    38641 accaaagcaa tggcgctgga gttggtcagg cataaaattt tggtgaacgc agtcgcacct
    38701 ggggcgatcg ccacgccaat gaacggcatg gatgatagcg atgtgaggcc cgacgcggag
    38761 ccttcgattc ccttgcgacg ttttggcaca acgcatgaga ttgccagcct ggtggtatgg
    38821 ctttgttcgg agggcgcaaa ttacaccacc gggcagtcgt tgatagtgga tggcggcttt
    38881 atgttggcga atccgcagtt caacccagaa tagcgcagtg aggtgcgtgg ttatttcaat
    38941 gggttaggcc agataagaca cgccagcgtc gcatcaggca tggaacatcc ggtgccggat
    39001 gtggcgtgaa tgcttatctg tggcaactgc tttacgcctg attatccggc tttttcttcc
    39061 cgcgtcgttt cagccaccag cgcacgccta ccaccagaac cacggccaga atcaaccaga
    39121 cccagtgctt caaatgctgg tcgagattgt gcaaccacgg cgcaatcacc tgaccaccag
    39181 cataaccgat agtggtaaaa atcaacgccc aggcaaatgc gccgagaata ttcagcggca
    39241 gaaagatttt cggcggcagc tggctggcac caatcagcgt cgggccaatc accctaaagc
    39301 catacataaa acgcgtacca atgacaaaca gatacggatg gcgttggata agtttctgcg
    39361 cccgctcaat tttatcctga tgtttcgaga aacggcgtaa tagcttgccg ccaaagcgcc
    39421 gcccgcacag ataaagcacc tggtcgccaa tcatgccgcc aagcgccaca gaaagtacca
    39481 ccagcgggaa ctttaatagc ccctgatgtg ccgcaacgcc tcccagcaaa gtcacggttt
    39541 caccttccgc caggctaccg atcaccagcg cggcataacc atattgtgag atgagtgtat
    39601 tgagatccat aggccagtct gtctcccttt gcttgcctgt aagcatacac cctgtgaata
    39661 gttcacgctg aaaaaggacg tacatcagac ggtgcaaatt acatgagcga acaaaaaata
    39721 atcacaagct gacttatact tgaactgtca ggttcatgaa ctcacaacaa gggggcggta
    39781 tgagccatgt ctggggactg ttttcccatc ccgatcgtga aatgcaggtg attaatcgcg
    39841 aaaacgaaac gatttctcac cattacaccc accacgtttt gctgatggcg gcgattccgg
    39901 tgatttgcgc cttcattggc actacacaga ttggctggaa ttttggcgat ggcactatcc
    39961 tgaagttatc ctggtttacc ggactggcgc tggctgtctt gttttacggc gttatgctgg
    40021 ctggggttgc ggtgatgggg cgggtcatct ggtggatggc gcgtaattat ccgcagcgtc
    40081 cgtcacttgc gcactgcatg gtcttcgcgg gctacgttgc aactccgctg tttttaagcg
    40141 gtctggtggc gctttacccg ctggtctggt tgtgtgcgct ggtcggcacg gtggcgctgt
    40201 tttacaccgg ctacctgctg tatctcggta ttccgagctt cctgaatatc aacaaagagg
    40261 aaggacttag cttctccagt tcgacactgg cgataggcgt gctggtgctg gaagtgctgc
    40321 tcgcccttac cgttatacta tggggctacg gttatcgttt gttctagaaa gttcttttgc
    40381 gctgcattgc tggcgtaaat gtcagcaatg cagcatttcc tcacgattct tctttgacga
    40441 tcgcactttt tgctcgttat gatggcgtcg ctagcctcag taaatcctta ctccggcggt
    40501 gcgcaacccg tgcgcgtgaa ccactatctg aatgctcatc atgccgaaat ttcgagtttc
    40561 tttatttagc ctggccctga tgctggctgt gcctttggca ccgcaggccg ttgcgaaaac
    40621 ggcagccgct accaccgctt cacaaccgga aattgcctcc ggtagcgcga tgattgttga
    40681 tctgaatacc aacaaagtga tctattcgaa ccacccggat ctggtgcgtc cgattgcgtc
    40741 tatcagcaaa ttaatgaccg cgatggttgt gctggatgca cgactgccgc tggatgaaaa
    40801 actaaaagtg gatatcagcc agacgccgga gatgaaaggg gtctattcgc gcgtacgact
    40861 gaacagcgaa atcagccgta aagatatgct gttgctggcg ctgatgtctt cagaaaaccg
    40921 cgcggcggca agccttgcgc accattatcc cggtggttac aaagccttta ttaaggcgat
    40981 gaatgcgaaa gcgaaatcgc tcggaatgaa caacacgcgc tttgttgaac cgaccggatt
    41041 gtcggtgcat aacgtttcaa ctgcccgtga cttaaccaaa ctgctcattg ccagcaaaca
    41101 atatccgttg attggtcagt taagtactac cagggaagat atggcaacct tctctaaccc
    41161 gacgtatacg ctgccgttcc gcaatactaa ccatctggtg tatcgcgata actggaatat
    41221 tcagttaacc aaaacgggct ttaccaatgc ggcgggccat tgtctggtga tgcgtacggt
    41281 tatcaataat aaaccggtgg cgctggtagt gatggacgcg tttggcaaat atacccattt
    41341 tgccgatgcc agccgcctgc gtacctggat tgagaccggt aaagtgatgc ctgttccggc
    41401 agcagcgttg agctataaaa aacaaaaagc cgcccaaatg gcggcggcgg ggcagacggc
    41461 acagaacgat taaatgaatg acggatggcg atactctgcc atccgtaatt tttactccac
    41521 ttcctgccag tttttccgct tactggtttt accaaccccc ggattcatac tgttggtcgg
    41581 atcgttctcg cgataaaact tctgcaacgt ttccggtgct ttatacaaat gaccgacgtt
    41641 atgctcggca gggtattgcg cgccgcgctg ctgtagcagt tccagcatct gttctttcaa
    41701 cgcatgcaca tccacgcctt ttttaacgat gtaatcctga tggaagacat agcacataaa
    41761 atggccgtaa tagagcttgt gcaccagctg gctgtcgatc tccggcggta aatgctcata
    41821 ccactcggtg tcgttacgcc gcagagcgat atccagtgcc agaatgtctt cgacttcatc
    41881 ggaatgcacc gcctgataac gaattgctgc gcccgcagcg gcgaaacggt gtaaaaacgc
    41941 tttgctgcct tcctccggcg tacagacaaa gaaatcgcct tcggcctgtt tgaaataatc
    42001 caccagccac gatttggctt caccgacgcc atcgcccgcc atttttaaca gcagatgatg
    42061 ttcgtattta tcgcgccagt ttttcatgcg cggcggtaaa tggctgggga acaggtgacc
    42121 gaatttttgc atcgcacggt cggtaaaatg cggacggaag aatttcactt tctccagcat
    42181 cgcatcggtg cgccccttga gattaaagaa gaacggcatc ttgtcggtgc ctaacttatc
    42241 aatcatcagg aaggtgtctt tgccgtattt ttccgctata tcgtagatat cccggtgcat
    42301 atattccccg gcaaccggca gattttcgaa attagccaga atatgacggc ggatttcggt
    42361 cagtacttcc ggctggttgg tgccgatata aaacacctgc tgattttttt ccgcttcgaa
    42421 ggtatcaaga cgtaccgcaa agacggccag cttcccggcg caaccagaag attcaaataa
    42481 ccgatccgga tcggcgttat aacgtgcggg cgtgtcggct tcaatatcac gaacgcggtg
    42541 gacatagtca taatcgtggg cgtgacgacc atcgtggcgc acatcgtcat ctttgatgcg
    42601 atcgtcatcc agcttgctaa ggatctgttc cggcgtttcg cccagatcaa tccccagatg
    42661 gttaaccagc gtcagcttgc catcttcatt tatacgcgcg aataacgaca tttcggtata
    42721 cgccgggcca cgttgcacca gcgagccgcc ggagttgtta caaataccgc cgatgaccga
    42781 tgcgcctata cacgatgatc caatcaccga gtgcggttcg cgtcccagcg gtttgagggc
    42841 tttttccagc gaataaagcg tggtgcccgg ataggccagc acctgttcgc ccttgccgag
    42901 aacgtgcagc ttgtcgagac gcagggtgct gatgatcacg atatcgcgat catagtcgtt
    42961 accgtttggc gtcgatcctt cggtcaggcc tgtattggca gcctgcatca gaataatttt
    43021 gtcggcggtg acgcaggctt tcagcacccg ccacaattcc agtagtgagc cagggaaaac
    43081 gacagccagt gcgtcgccct gaccagaacg gaagcccttg cgatagcggg ccgtttttgc
    43141 gggatcggtg agcaggtgtg aatgaccgac cagacgggca agttcattca aaaaggcttt
    43201 attatcagtt gttgtcatgg aagacatttt ccactccttg tggtggcgaa aaaatatcac
    43261 aaactagaat agcgccttgt atgacaattc agcgagtgaa tattttgaaa attcagacaa
    43321 taaactgagc attctttttc tctctccatc atgcttatgg caaactacgg tttttgcacc
    43381 attttcgctg ccacgtcggg caacaaagga agaaaaatcc atatgaaatg gctatgttca
    43441 gtaggaatcg cggtaagtct ggccctgcag ccagcactgg cggatgattt attcggtaac
    43501 catcccttaa cgcctgaagc gcgggatgcg ttcgtcaccg aactgcttaa gaaaatgacg
    43561 gttgatgaga aaattggtca gctgcgctta atcagcgtcg gcccggataa tccgaaagag
    43621 gcgatccgcg agatgatcaa agacgggcag gttggggcga ttttcaacac cgtaacccgt
    43681 caggatatcc gcgccatgca ggatcaggtg atggaattaa gccgcctgaa aattcctctt
    43741 ttctttgctt acgacgtgct gcacggtcag cgcacggtgt tcccgattag cctcggtctg
    43801 gcctcgtctt ttaacctcga tgcggtgaaa acggtcggac gtgtctctgc ttatgaagcg
    43861 gcagatgatg gcctgaatat gacctgggca ccgatggtcg atgtctcgcg cgatccgcgc
    43921 tggggacgtg cttccgaagg ttttggcgaa gatacgtatc tcacttcaac aatgggtaaa
    43981 accatggtgg aagcgatgca gggtaaaagc ccggcagatc gttactcggt gatgaccagc
    44041 gtcaaacact ttgccgcata cggcgcggta gaaggcggta aagagtacaa caccgtcgat
    44101 atgagtccgc agcgcctgtt taatgattat atgccgccgt acaaagcggg gctggacgca
    44161 ggcagcggcg cggtgatggt ggcgctgaac tcgctgaacg gtacgccagc cacctccgac
    44221 tcctggctgc tgaaagatgt tctgcgcgac cagtggggct ttaaaggcat caccgtttcc
    44281 gatcacggcg caatcaaaga gctgattaaa catggcacgg cggcagaccc ggaagatgcg
    44341 gtgcgcgtgg cgctgaaatc cggcatcaac atgagtatga gcgacgagta ttactcgaag
    44401 tatctgcctg ggttgatcaa atccggcaaa gtgacgatgg aagagctgga tgacgctgcc
    44461 cgtcatgtac tgaacgttaa atatgatatg gggttgttta acgacccata cagccatttg
    44521 gggccgaaag agtctgaccc ggtggatacc aatgccgaaa gccgcctgca ccgcaaagaa
    44581 gcgcgtgaag tggcacgcga aagcctggtg ttgctgaaaa accgtctcga aacgttaccg
    44641 ctgaaaaaat cagccaccat tgcggtggtt ggcccgctgg cagacagcaa gcgtgacgtg
    44701 atgggaagct ggtccgcagc cggtgtcgcc gatcaatctg ttactgtgct aacagggatt
    44761 aaaaacgccg tcggtgaaaa cggtaaagtg ctgtatgcca aaggggcgaa cgttaccagt
    44821 gacaaaggca ttatcgattt cctgaatcag tatgaagaag cggtcaaagt cgatccgcgt
    44881 tcgccgcaag agatgattga tgaagcggtg cagaccgcga aacaatctga tgtggtggtg
    44941 gctgtagtcg gtgaagcaca ggggatggcg cacgaggctt ccagccgtac cgatatcact
    45001 attccgcaaa gccaacgtga cttgattgcg gcgctgaaag ccaccgctaa accgctggtg
    45061 ctggtgctga tgaacgggcg tccgctggcg ctggtgaaag aagatcagca ggcggatgcg
    45121 attctggaaa cctggtttgc ggggactgaa ggcggtaatg caattgccga tgtattgttt
    45181 ggtgattaca acccgtccgg caagctgccg atgtccttcc cgcgttctgt cgggcagatc
    45241 ccggtgtact acagccatct gaataccggt cgcccgtata acgccgataa gccgaacaaa
    45301 tacacgtcgc gttattttga tgaagctaac ggggcgctgt atccgttcgg ctatggtctg
    45361 agctacacca ctttcaccgt ctctgatgtg aaactttccg cgccgaccat gaagcgtgac
    45421 ggcaaagtga ccgccagtgt gcaggtgacg aacaccggta agcgcgaagg ggcgacggta
    45481 gtgcagatgt acttgcagga tgtgacggct tccatgagtc gtccggtgaa gcagctgaaa
    45541 ggctttgaga aaatcaccct gaaaccaggc gaaacccaga ccgtcagctt cccgattgat
    45601 atcgaggcgc tgaagttctg gaatcaacag atgaaatatg acgccgagcc tggcaagttc
    45661 aatgtcttta tcggcactga ttccgcacgc gttaagaaag gcgagtttga gttgctgtaa
    45721 tgctttagta agggcgcgac gtctgcgccc tttaagtatt tatctgattc tgcaactgaa
    45781 tccgcccaga caggctaacc ctgtcattat caactatgct tttctcttaa ttcgctgaaa
    45841 acaacagtaa aagaatgagg aaagcatcat gccactctca aagctctggg caggttcatt
    45901 ggttttgttg gcagccgtga gcctgccgct acaggcggct tcccccgtta aggtcggttc
    45961 aaaaatcgat accgaaggcg cgctgctcgg caatatcatt ttgcaggtgc tggaaagtca
    46021 cggtgttcct acggtaaata aagtgcaact gggaacgact cccgtggtgc gcggggcgat
    46081 tacttccggt gaactggata tctatccgga atataccggc aatggcgcgt ttttctttaa
    46141 agatgaaaac gatgcggcat ggaaaaatgc cgggcaaggc tacgagaaag tcaaaaaact
    46201 cgatgcagag caaaacaagt taatctggct gacgcccgcg cctgcaaata acacctggac
    46261 tatcgccgtg cgtcaggatg tggcagagaa aaacaaactc acttcgcttg ccgacctgag
    46321 tcgttatctg aaagagggcg gcaccttcaa actggcggcc tcggcagaat ttattgaacg
    46381 cgccgatgcc ttacccgctt ttgaaaaagc ctacgacttt aaactcgatc aggatcagtt
    46441 gctgtcactg gctggtggcg acacggcggt aacgatcaaa gccgctgccc agcaaacttc
    46501 tggcgttaat gccgcaatgg cttacggcac tgacggtccg gtcgcagcgc tggggttgca
    46561 aaccttaagc gatccgcaag gcgtgcaacc tatctacgcg cctgcaccag tggttcgtga
    46621 atcggtgttg aaggagtatc cgcaaatggc acagtggcta cagccagtct tcgccagcct
    46681 cgatgcaaaa acattgcagc aactgaatgc cagcattgca gttgaaggac tggatgccaa
    46741 aaaagtggct gccgactacc tgaaacaaaa agggtggacg aagtaatttc ccgtgactta
    46801 tctacgtatt aatcctgttc tggcgctgct gctgttgctg acggcaatcg cagcggcgct
    46861 gccgtttatc agttacgcgc ctaatcgttt agtttcgggt gaggggcgtc atctctggca
    46921 gctgtggccg caaacgatct ggatgctggt gggcgttggt tgcgcctggc tgaccgcctg
    46981 tgttattccc ggtaaaaaag gcagcatttg tgcactcatt ctggcgcaat tcgtcttcgt
    47041 attgctggtg tggggagctg gaaaggcggc gacacaactg gcacaaaatg gcagtgcgct
    47101 ggcgcgtacc agcctcggca gtggtttctg gctggctgcg gcgctggcat tgctggcctg
    47161 tagcgatgcc atccgccgaa tctccacgca tctgctgtgg cgctggttgt tgcatatgca
    47221 gattgccatt attccgctgt ggttgctgta ctccggcacg cttaacgatc tctcgctaat
    47281 gaaagaatac gccaaccgtc aggatgtgtt tgacgacgcg ctggcacaac atctgacgtt
    47341 gctgtttggt gcggtgctgc ctgcgttagt gattggtgtg ccgttgggcg tctggtgcta
    47401 cttttccact gcgcggcagg gagcgatttt ttcgctactc aatgtcattc agaccgtgcc
    47461 ttcggtggcg ctctttggcc tgttgattgc gccgcttgcc gcgctggtta cggcctttcc
    47521 gtggctgggt aagctcggca tatcaggaac cggaatgaca cccgcactga ttgcgctggt
    47581 gctctatgcc ttgctgccgc tggtgcgcgg cgtggtagtc ggcttgaacc agatcccgcg
    47641 cgatgtgctg gagagcgcca gagcgatggg catgagcggg gcgcagcgat tcctgcatgt
    47701 tcagttaccg ctggcgttac cggtatttct gcgtagcctg cgggtggtga tggtgcaaac
    47761 cgtcggcatg gcggtgattg cggcgttaat cggcgcaggc ggttttggtg cgctggtttt
    47821 ccaggggctg ctaagcagtg ccattgattt agtgttgctg ggggtgatcc cggtaattgt
    47881 tctggcggtg ctgatcgatg cgctgttcga tttgcttatc gcactgctga aggtgaaacg
    47941 taatgattga atttagccat gtcagcaaac tgttcggcgc acaaaaagcc gttaacgatc
    48001 tcaatctcaa ttttcaggag gggagttttt cggtgctgat tggtacatca ggctccggta
    48061 aatccaccac cctgaaaatg attaaccgcc tggtggagca tgacagcggc gtgatccgct
    48121 ttgccggaga agaaattcgc tcgctgccag tgctggagtt gcgccgccgg atgggctatg
    48181 ccattcaatc tattggcctg tttccccact ggagcgtggc gcaaaatatt gccaccgtgc
    48241 cgcaattaca aaaatggtca cgggcgcgga tcgacgatcg tatcgacgaa ttaatggcgc
    48301 tactggggct ggagccaaat ttgcgtgagc gttatccgca tcagctttcc ggtggtcagc
    48361 agcaacgtgt gggagtggcg cgcgcactgg ctgccgatcc gcaagtctta ctgatggatg
    48421 aaccttttgg cgcgctggac ccggtaacgc gcggcgcgtt gcaacaagag atgacgcgca
    48481 tacaccgttt gctggggcgt accattgtgc tggtcactca tgatattgat gaggcgctac
    48541 gactggcaga acatctggtg ttgatggatc acggtgaagt ggtgcagcag ggcaatccgc
    48601 tgacgatgct gactcgtccg gcgaatgatt ttgtccgcca gttttttggg catagtgaac
    48661 tgggtgtccg cctgctttcg ttacgtagcg tggcggatta cgtgcgtcgc gaagaacggg
    48721 cagaaggtga ggcgctggca gaagagatga cgctacgcga tgcgctctcc ctgtttgtcg
    48781 cgcggggatg tgaagtgctg ccggtggtga acacgcaggg cgagccttgc ggcacgctgc
    48841 attttcagga tctgctggtg gaggcgtaag cgtatgaaga tgttgcgcga tccgctgttc
    48901 tggctcattg ctttgtttgt ggcactgatt ttctggctgc cttacagcca gccgctgttt
    48961 gctgccttgt tcccacaact gccacgcccc gtttatcagc aagaaagttt tgctgctctg
    49021 gcactggctc atttctggct ggtgggaatt tcgagtttgt ttgcggtgat cattggcact
    49081 ggtgcaggaa ttattgttac tcgcccgtgg ggcgcggaat ttcgcccact ggtggaaact
    49141 attgccgccg ttggacagac ttttccgccc gtcgcggtgc tggcgattgc cgttccggtg
    49201 attgggtttg gtctgcaacc cgcgattatc gccttgatcc tttatggtgt gctgcccgtc
    49261 ctgcaggcga cacttgccgg gctgggagcg attgatgcca gcgtgacaga agttgcgaaa
    49321 agtatgggaa tgagtcgagg tcagcgactg cgtaaggtcg agctaccgct ggcggctccg
    49381 gtgattctgg cgggtgtgcg aacttcggtg attatcaaca ttggtacggc gacgatcgcc
    49441 tcaacggtag gggccagcac gctgggtacg cccatcatca tcgggcttag cggatttaat
    49501 accgcgtatg tgatccaggg ggcgttactg gtggcactgg cggcgatcat tgcagaccgc
    49561 ctgtttgaaa ggctggtgca ggcgcttagc cagcacgcaa aataaaggta taacctgcga
    49621 gcatgacgcc accaattccg cctaacgcca taaacaggaa cagggcgatg accccaattt
    49681 tagctatgcg cataatgcac tccttatgtt aacgaaagga ttgtacagta aagcgcattt
    49741 gttaacgaat cattaaatgc cgagtgggaa aatatcatgg ccttgttcct gccaactggt
    49801 gagttgctgc tgttgggcgg aggttcgatt ttcaccgcac cacaccagca atgtacggcc
    49861 ttcgaacagt tcagggcgta gttgattgag cgagtgggcg aggacatcaa tgcgccatcc
    49921 ttgttgactg gcaatccagc cctccagcca cagacgggtg gtatcgtgaa tattccagcc
    49981 aaccaccagc gcatctttac cctgtttttt acgtgccgaa gccaggcaaa tggcgatgta
    50041 gttgatcagt acgccgtcga ggatcgccag cagcgcctgg agagtcggtt gttggcactg
    50101 aagtcgtcgg cgcagaggaa taaacagatg cgtggtgagt gtctgggcgg ggtaatcctg
    50161 accgcgctct ttgatccacg ttcgcaggct atgcagattg ccgctttgca ggtaagtcag
    50221 taatgtttct tgctgatcgc gccagccgtt ctgcacatca acattttcat tactgagcag
    50281 cattttaact ttgctgacct gcacgccgtt gtcgatccag cgtttgatct cgcggatccg
    50341 gtcaatatcg gcatcattga acagccgatg accgccgtct gtccgttgcg gtttcagcaa
    50401 tccgtaacgc ctctgccacg cgcgtaacgt gacagggtta atatcacaaa gcaacgccac
    50461 ttcaccaatt gtgtaaagcg ccatcgtctc acccttgctc gcgaggtccc ggtttaactt
    50521 tagacgcagt tttgcgaacc aggtagtttt gcccgttttt tgtgcatcta tagggtgatt
    50581 ttatttttgc caggcgattt tgagtgatcg tactcacgaa ttctcatttt tctgcaagag
    50641 ttcaaagaaa gttaaacgca ggcaatgtat gttacgcgtt ttaaagggaa gtgtggtttg
    50701 cgggtatgta cgattttaat ctggtgttgc tgctgcttca gcagatgtgc gtttttttag
    50761 tcattgcgtg gttaatgagt aaaacgccat tattcatacc gttaatgcag gtcacggttc
    50821 gtctgccgca taaatttctc tgctacatcg tcttttccat cttctgcatc atgggcacct
    50881 ggtttgggct gcatattgac gattctattg ccaatacccg tgcgataggc gcggtaatgg
    50941 gcggcttact cggcggtccg gtcgtcggtg ggctggttgg tctgaccggc gggttacatc
    51001 gatattcgat ggggggcatg accgcgctaa gttgcatgat ctcgaccatc gttgaagggt
    51061 tgctcggcgg cctggtacac agcatcctga tccgtcgcgg gcgcactgat aaagtcttta
    51121 accccattac cgccggtgcc gtcacgttcg tcgctgaaat ggtgcaaatg ttgatcatcc
    51181 tggcgatcgc ccgaccttat gaagatgcgg tgcgtctggt gagtaatatt gctgcgccaa
    51241 tgatggttac caataccgtc ggcgcggcgc tgtttatgcg tatattgctc gataaacgcg
    51301 cgatgtttga aaaatacact tcggcttttt ctgccactgc gctaaaagtg gctgcctcga
    51361 cggaaggcat tttgcgccag gggtttaacg aagtgaacag catgaaagtg gcacaggtgc
    51421 tgtatcagga gctggatatt ggtgcagtcg cgattaccga tcgagagaaa ttgctggcct
    51481 ttaccggaat tggtgacgac caccatttac ccggcaaacc gatttcttcg acttacacct
    51541 taaaagcgat tgaaaccggt gaagtggtct acgctgatgg caacgaagta ccttatcgtt
    51601 gctctttgca tccgcaatgc aaactggggt cgacgctggt cattccgttg cgtggcgaaa
    51661 atcagcgggt gatgggcacc atcaaattgt atgaagccaa aaaccgttta ttcagttcaa
    51721 tcaaccgcac gttgggcgag gggattgcgc aactgctttc ggcgcagatc ctcgccgggc
    51781 aatatgagcg gcaaaaagcg atgctcaccc agtcagagat caaactgctt cacgcccagg
    51841 tgaaccccca ttttttgttt aatgcgctta acaccattaa agcggtgatc cgccgcgaca
    51901 gcgaacaggc cagccagctg gtccagtatc tttccacttt tttccgcaaa aacttaaagc
    51961 ggccttcgga gtttgttact ctcgcagacg aaattgaaca tgtgaacgct tatctgcaaa
    52021 ttgaaaaggc gcgcttccag tcgcggttgc aggtcaacat tgctattccg caagaattat
    52081 cccagcagca attgcccgcg tttaccctgc aaccgatagt ggaaaacgcc attaaacatg
    52141 ggacatcaca actgctggat acagggcgag tggcgatcag cgcccgacgt gaggggcaac
    52201 atttgatgct ggagatcgaa gacaatgccg gtttgtatca accggtaacc aatgccagtg
    52261 ggctggggat gaatctggtg gataagcgtt tacgtgaacg gtttggcgat gactatggga
    52321 taagcgtcgc ctgtgagcct gattgttaca cccgaataac gttacgacta ccatggaggg
    52381 acgaggcatg attaaagtct taattgtcga tgatgaaccg cttgcacggg agaacctgcg
    52441 cgtatttttg caggagcaga gcgatattga aatcgttgga gagtgttcaa acgccgtaga
    52501 agggatcggc gcggtgcata aactgcgccc ggatgtgctg tttctcgata tccagatgcc
    52561 gcgcatcagt ggtctggaaa tggtggggat gctcgacccc gaacatcgac cgtatatcgt
    52621 ttttctcacc gcgtttgacg aatacgctat taaagccttt gaagaacatg cctttgacta
    52681 tctgctgaag ccaattgatg aagcgcgact ggagaaaacg ctggcgcgtt tgcgtcagga
    52741 acgcagtaag caggatgttt cgctgttacc ggaaaatcaa caggcgctga aatttatccc
    52801 ttgtacgggg catagtcgga tttatttact gcaaatgaaa gatgtggcat ttgtcagcag
    52861 tcggatgagc ggtgtctacg ttaccagcca cgaagggaaa gagggcttta ccgaattgac
    52921 attacgtacc ctggaaagtc gtacaccact actgcgctgc catcgtcagt atctggttaa
    52981 cctcgcgcat ttacaggaga ttcgtctgga agataacggc caggccgagt tgattttgcg
    53041 taatggctta accgtgccgg tcagccgccg ttatctgaaa agcttaaaag aggcgattgg
    53101 cctgtaaaag actgctaaaa tggctttttg cctcatcaac acctgaaggc ctcatgctaa
    53161 gtaacgatat tctgcgcagc gtgcgctaca ttttgaaagc caataataat gacctggtgc
    53221 gtattctggc gctgggtaat gtcgaagcca ccgcggaaca gatcgccgtc tggctacgta
    53281 aagaagacga agagggtttt cagcgttgtc cggacattgt tttgtcgtca ttcctcaatg
    53341 gcctgattta tgaaaaacgc ggcaaggatg agtctgctcc ggcactggag ccggaacgtc
    53401 gcattaataa caacatcgtg ctgaaaaaat tacgcatcgc gttttcgctg aaaaccgatg
    53461 acattctggc gatcctcacc gaacagcagt tccgcgtttc gatgccggaa attacagcga
    53521 tgatgcgcgc accggatcat aaaaacttcc gcgaatgcgg cgatcaattt ttacgttatt
    53581 ttctgcgtgg actggcagcg cgccagcatg tgaagaaaag ctaagacggg tatggcggcc
    53641 atgcaaaaac atggccgccg acagattatt tcacttcttt aaaaccagcg gctttcatca
    53701 ccagttccat ttgcgccata gtgatacctt ttttggcatc ttcagcagaa acgttgattc
    53761 ctgaaatacc ctgcagggct ttgaaatcca ctttttccat atcgatagtc acgttttcct
    53821 gcgcgtaagt atcggtatag gttaattttt cttcaacacc cgcgatgttt ttgtatttgg
    53881 cgcttaacgg ctcaagtgtc ctggcagcgt cttctttggt ggttgcacca atggaggcaa
    53941 attgaatttt ggtttcagaa gattgcttaa gcaccttgtc acctttgtag acataggtaa
    54001 tggcaatttc agtaccgttc agattggcgc tgaatttttt cgattcttct ttgtcaccgc
    54061 agccagcaag agagaaaacc agaacagatg caacaacgag ggaaaacagc ttattgaaag
    54121 ccttcatgta aaactccatt ttatttaatc aaggaactgg tgactctcac caggggctat
    54181 ataggatatg cctaataccg tggcgtgagc agtccggaac tggagtagaa ctcttagtaa
    54241 aaagcactat ttcatccttg ttgctgaagc atggggaata attgttcgca cagcaaaaca
    54301 ccgttattca ttgcttctac ccgtgcctcg ctttctgtat tacgaaattg tcccaacaca
    54361 tgtgccagcc gataaaaacc caccgcggtg aggtcattcg ccagcaactc tgcctgacca
    54421 atagcactat gttcctgata gcgccagccg ttatggagca gttgaataag taacgcctgg
    54481 cagcgcatca gcaactgatg agcagtagac ggcacaggca gaacgctggc agaaggtagt
    54541 gatgccacca caggcgcagt ttctgcgtcc agcgcccagg cacgagtctt tgtcatcatt
    54601 acctgtggtt ccagtgtcaa ttgcccctct acaaagctga caaagccaga aaccagacac
    54661 acggggtcgt ctgtttgttg caaaagcgcc gccatgcgtt caacggcata aggcgcgctg
    54721 gctgaggctg gcagtgataa cgtcagcaga ttatcttcac cttcgccgct gattacctgc
    54781 gcatccagcg tctggcggct gctatcccaa ccgagcgaaa tacactcagc gaccggcaga
    54841 ataaataagt tatcgacctg attaagaggc cgtatgcagg cggggggacg ctggcgtaaa
    54901 tattcccgca aagccacaat gcccggctgg cgtaacggcg cgctcaacat ttgccaggca
    54961 tcaggcgaca gcggcacaac gctgcttaag cggttgcggg tagctaacag cagctcgcca
    55021 tcggcactgc gttttgctgc ttgtgaaaca atttgcccgc ccgccagtgc gccagcctga
    55081 aaactaaaca gccgacgcgt cgctgccggt gagttttcct gttcgcttct cggccaacta
    55141 cgcgaaagat gcaaaatact gccggtgtcg ggatcggtaa accagatgcg taaaccataa
    55201 tgctcaatat cctgccagca acgcatacct aaagacacca gccgcagatg atcaagcttt
    55261 gcttctccgg caatgccaga gccaacgacc gtgcgccacg gcatgggcgg tacttcaccg
    55321 acactgtcac gtcgcgctat ctcttgtgca ctgttcaagc gactggttaa cgccgcaagc
    55381 tggcacaagc attttccggc atgatagtgg ctggcgcggg cgtggaaggc atcaacgctg
    55441 gcgcgcagtt gccgtagcga ttcactcacc catcgccagt tgcagcgttc cgccgcctgc
    55501 tgtgcgcggc tgaaagcggc ctcgtaatgg attggcggct ggctgatgcc gcccagccat
    55561 aatgcctgac ttaattgctg aacatattga cgacacgcgt tgccctcttc gctggcaaac
    55621 ggatcgtcag atgatgtgac gtgttcgctg cgcatctgcc agattaaatg agtaaattcc
    55681 gcttgctgag ttttggcctc gacgaaggcc tgcacagcca gtacgacatg ttcgcaaagt
    55741 gtgccgtcaa tacaatcaca gcgagcgaaa cgaatgctgc tgcgggaata aaaacgtaca
    55801 tcgctcatcg gtaagcgggc agagggaatt tcgcccggcg tacagaacaa ctcaatggtg
    55861 atgcctttag cgaccagcgc ctgtgcgcgt ttgcgggtgg catcgggcag ggtagccagt
    55921 tcttccagcc agattgccgg atcccactct tcttcttttt ccgtaggctg ggtggtggcg
    55981 caaagtcgct gataacttaa aaccagcatc acgcgatggc ggcacatgcc gctggccccg
    56041 cagctgcact gagcctcttt cagtgcctgg ccgttcgcca gctgggtgcg gacaccgtca
    56101 ctgaaggtgg cgattaaagc gccgttctca tgactgattt ccgggacgtt gccattttcc
    56161 agttctttaa ggctgcgctt aacaaaaccg gcattgctta acgccgtcag tgcctgtggt
    56221 gtcagttcta ataattccgg acgtagtgaa ttcatgactg aagattctcc gcaagccagg
    56281 tcgccagttc gcccggcgtc atggcggcta tttgtgcacc gacattaacc agtgcctggg
    56341 ccatatcgcg gtcatagcaa ggtgttgcgg tgctatcgag cgccgccagt cccagcactt
    56401 tgatgccgct ctggacacac tttttcacct gatgcgtcag caatgatgat gaaccacctt
    56461 catagaaatc gctcacgagg ataatgacac tttttgcagg ttgttcaata agttgccgac
    56521 catactccac ggcactggcg atattggtcc cgccgcccag ttgtactttc attaataact
    56581 ctaccggatc ggcaacgtct gccgtgagat caaccacgct ggtgtcaaac gccaccagat
    56641 gggtacgaat gccgggtaac tgccataaac aggccgccat caccgctgag tggatcaccg
    56701 agtcaaccat cgatccgctt tgatcaacca gtaagaccag ttgccattga tcgctatggc
    56761 gcttaatgcg gctgttaaag cggggggatt cgatatacaa cttgccgtgt tgcggatgcc
    56821 agtgttgcag gttggcacgc agcgtacttt tgaaatcgaa attacgcgcc agtggaataa
    56881 atgagcgacg gcggcgatcg cggacaccag aaaaagcctg acgaacttcc tttgccagtc
    56941 gcgccataat ttcttcaaca acctggcgca ctattcggcg ggcggcagcc agcacttcgg
    57001 gattcatcag atgtttggtg tgcaaaacgg cgcgtagcag gctttcagaa ggctgcatac
    57061 gttccagcac gtcgagattc gtcaccacat cttcaatgcc gtagcgcagt acggcatcgc
    57121 tttccagtcg ctcgatcacc cgttgcggaa acagcgtgtg aatactgttg atccactcag
    57181 gggtggtgag atttgagtca cctaatccac cggagcgttc accacgctgg agccgttcag
    57241 gatcgcgccc atacagccac tccagcgcgt ggtctatctg ccgggcgttg tcatccagcc
    57301 cacaaagcgt cgtttctgcc gcttcgccaa gaattaatcg ccagcgttgt agctcacggg
    57361 tggtcagaag atcgttcagt tcagacatgg aaaactcccc agtgttgcag cgatgccagt
    57421 gcctgctgtt cgagttgttg atgatgtgca atgtcttgtg gtggacaatg caacggcatt
    57481 tgcagtgccg aaacgggaag ttgcaccagt tgataatgct caagcacctg atgcgccagc
    57541 gtcccgcgtt ctcgtggtgg tagccaggcc atcgccgcgc gtaaatcggg cagggcattg
    57601 ataaaatcgg catcactcag ttgatttaaa tgactgctga aaccggcgat aaataccggc
    57661 tgacaggcca gttgatgacg ggctaatgcc agcaaaccgt gcagcgcctc gccggattgt
    57721 gtcggggata attgcaccag cattgtcagc gccgcttcgg cactggcgtt gggatgttcc
    57781 agacgcatca gtgcgccaag tgccgcgccg cgatccagcg ccggggcatg aatcgcctga
    57841 ctgcgtcgct ccagtaaggc taccgccgca gaaagagaaa cgccgggtaa attaacgccg
    57901 ctatgtagat cgcgcaggat atggcaaagc gcttgccagc tatgcaggtg agcgtgaaac
    57961 tccttttcat ccggtctgcc gttggattca catagccaca gcgtgcgatc gatggccgcg
    58021 cataatgtcg tttgtaatat ctgcgcgccc tgcataccgc taatttcatc cagccgccat
    58081 aaggcatata gcacttccag cgcctggccc atttcggcaa actgattttc ctgggcgatt
    58141 aataatgtga gttgctccag taattgttga ttgaaggacg ccagacccgc taacgccgcc
    58201 tggctaagac atgtggtgat actgccgatt ccacccgcgt ccagcatatc ggcttctaat
    58261 ttattgcgtg cggcttcctg gagtgtggca ccaaagcagg cggcctcaat taatgccgca
    58321 tgttggctaa gcgggcgggt taatttccag cgttcttcac cgttgcctgc aagtgtcagt
    58381 gtacttccct gctggcgcac aacccctggg atctccagta ttaccagccg atgtaacacc
    58441 tgactttgcg ccagtccatc gggggtaaag cgatttaaag taagctccgc cggaaaagag
    58501 attcctgtac gttccagttc gcaggtgaca tcttttggca gaggcggctg tggcgtagag
    58561 ggggcaagtt taccgaatcc gtcacccgct aatgtgtcta tcaacgttag cagaatcgga
    58621 tcggtatcgg gatgaataac gccgcgatag ctccacggca acggcgcatt cagggcttct
    58681 ttaatcagcg agcctgctat ggcatccagc cagtcagtgc gtaacggtag tgtatgacca
    58741 cgcaactgtg ccagtgccat cgcatgcaga tgagcggcag ccatatccgc tgttgaagca
    58801 ggcaagtggt gctggcgcaa acgggtaaga accgttttca gcagttgttc acctgcctgc
    58861 tgcaagcccc accgccagca ccagttttgc cagaccgggg caggcatacc ggaaaggtat
    58921 cctgccagca catcaaggcg cttttcactg tagggggtga gataacaacc tgtaacggca
    58981 tctgccagcg agggcaattc cggcgtgtta atttcctgcg ggcattcgcg ccacattttt
    59041 gccagtgcag aagcgtgcca gccaccgcag acgactaaca cgtcgccatt gttttgctgc
    59101 atcgcccaac caatccagcg ggccataaag tcttcgcgct gacgattgag agcatctccg
    59161 ggggaatcgc cccgcagttg tgcaaaatag tgcgccagtg cagagggtaa tgcagtttgc
    59221 tggctttcat cttcgaacaa gtgatcccac agggtgtcgc tgttatccat gcgggtggca
    59281 cgcagcagca aggtctggct ttcctcttgc gtatcaggcg aatcgtcctc ttcttcactc
    59341 tgcgcccagc aaggcaaatc gatgaagtaa gtttgtgcct gaatacgacg agcggcttgt
    59401 agcgcctgcc actccggcga aaattcagca tatggcgtcc aggcaccacg cccgggggct
    59461 gcaccgtcct gatactggca ataactgtaa atagctaccg gaagctggtg ggctaaaaac
    59521 agttcgtcta cccggtcatt aaaatcagcc gggccttcaa tcaacacgta tcgtggccgc
    59581 tggctttcga ttaacgattt caccagccgg gcgcaggccg gactatgatg ccggatgccg
    59641 acaattaacg gctcgctcat gcatctttcc tcacggcagg cggtggcgag cttgataata
    59701 agcctgccag tgagcttctt tatgtgtggc gactcgctgt tcgaagtaac ggcgcagacg
    59761 agcgcgatct tcttcgttat ctttgacgat ggttccggca atacagtcca ccagatcggc
    59821 tggctcacct gcgcgattcg ctaaaaacca ggcgcggacg cccactgcgt gggccacgtt
    59881 cacggcttca gcggtggaca taattgccgt cagggtatcc attgacgttt tcttttcgcc
    59941 gttggcacgc agatcgcgga aggtgcggac cagtaactcc agtaccgcgt caggcacttt
    60001 atgcggaata ccgctatggg ccagcaaacg cgccgaagca ctggcgacca gttccagctc
    60061 ctgggcgaaa tccataatcg gaaagacggt ttcaaaatcg aagcgacgct ttagcgcggc
    60121 actcatctca ttgacgccgc gatcgcgggt attggcagtg gcaataatat taaagccttc
    60181 tcgcgcatag agctgactgg cttcaccggt gagttccggc accgtcatta ccctgtcaga
    60241 aagcatacct aacagacagt cttgtacttc cagcggtgta cgcgttatct cttcaaaacg
    60301 gacaattttg ccgtcgcgca ttccctggta aagcggggca gggaccagcg cttccgttga
    60361 cggaccatgg ttgataagca gggcgtagtt ccagccgtac ttgatttgat cttcggtggt
    60421 agatgcgccg ccctggatgg ttaacccggc atcgccgcta atagcggttg ccagtaattc
    60481 agacaggagc gattttgcgg tgccaggttc ccccaccaac atcaaaccgc gcccggtagc
    60541 gagagtgact aacatgcgtt cgacagcgga gggactgatc acgactttac ggctaatatt
    60601 ttgcgcgcta tcgccaagga taaaagcgcg ggccgcaggc aaacttaact gccaaccggg
    60661 cgggcaaggt gcgtgatcat tttgttttaa ttttgccagt tcatcggcgt ataacacagc
    60721 ggcaggcgga cgctgcagat gattattctg cggtgacata tattcatccc tgaaatgtat
    60781 taatgaaata aacgatattt tacgcgcaat aaatgtttgc cgcattagtt gtttgagcga
    60841 aaaatatctg gaaagcagat cacagtaaat aaaaaaacct gctggtgagg caggttcttt
    60901 tatttactac aatgacgcta acttgctgat tgtgcaacat ttcgtaccaa cgaataaatt
    60961 ataaccatat ccacactcca ttgcaattca gtgaatgaga tttataatcc tgcccgttgg
    61021 gaggttgtgc cggatcggac caattttaag tcctggctgc tggacgggtg gtgccgcagg
    61081 cggtgccctg atccggagag caacgtttcg taccaacata cgtaaaccag tatcgacagg
    61141 cagt
//
LOCUS       DAZUCC010000003        60400 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZUCC010000003 DAZUCC010000000
VERSION     DAZUCC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.003"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..110)
                     /locus_tag="ACHY09_000117"
     CDS             complement(<1..110)
                     /locus_tag="ACHY09_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XapX domain-containing protein"
                     /protein_id="HFS3883141.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILS"
     gene            complement(163..1419)
                     /gene="xapB"
                     /locus_tag="ACHY09_000118"
     CDS             complement(163..1419)
                     /gene="xapB"
                     /locus_tag="ACHY09_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFS3883142.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(1480..2313)
                     /gene="xapA"
                     /locus_tag="ACHY09_000119"
     CDS             complement(1480..2313)
                     /gene="xapA"
                     /locus_tag="ACHY09_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFS3883143.1"
                     /translation="MSQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            2561..3325
                     /gene="yfeN"
                     /locus_tag="ACHY09_000120"
     CDS             2561..3325
                     /gene="yfeN"
                     /locus_tag="ACHY09_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFS3883144.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(3363..4289)
                     /gene="yfeR"
                     /locus_tag="ACHY09_000121"
     CDS             complement(3363..4289)
                     /gene="yfeR"
                     /locus_tag="ACHY09_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3883145.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFILHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWEVVRGQGRALMAGREGDPLYQI"
     gene            4379..5377
                     /gene="yfeH"
                     /locus_tag="ACHY09_000122"
     CDS             4379..5377
                     /gene="yfeH"
                     /locus_tag="ACHY09_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFS3883146.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(5374..5592)
                     /gene="ypeB"
                     /locus_tag="ACHY09_000123"
     CDS             complement(5374..5592)
                     /gene="ypeB"
                     /locus_tag="ACHY09_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFS3883147.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5594..7609)
                     /gene="ligA"
                     /locus_tag="ACHY09_000124"
     CDS             complement(5594..7609)
                     /gene="ligA"
                     /locus_tag="ACHY09_000124"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS3883148.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHEVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAVKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(7680..8678)
                     /gene="zipA"
                     /locus_tag="ACHY09_000125"
     CDS             complement(7680..8678)
                     /gene="zipA"
                     /locus_tag="ACHY09_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFS3883149.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQLVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            8908..9669
                     /gene="cysZ"
                     /locus_tag="ACHY09_000126"
     CDS             8908..9669
                     /gene="cysZ"
                     /locus_tag="ACHY09_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFS3883150.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            9854..10825
                     /gene="cysK"
                     /locus_tag="ACHY09_000127"
     CDS             9854..10825
                     /gene="cysK"
                     /locus_tag="ACHY09_000127"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS3883151.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11209..11466
                     /gene="ptsH"
                     /locus_tag="ACHY09_000128"
     CDS             11209..11466
                     /gene="ptsH"
                     /locus_tag="ACHY09_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFS3883152.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11511..13238
                     /gene="ptsI"
                     /locus_tag="ACHY09_000129"
     CDS             11511..13238
                     /gene="ptsI"
                     /locus_tag="ACHY09_000129"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFS3883153.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     TDLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13279..13788
                     /gene="crr"
                     /locus_tag="ACHY09_000130"
     CDS             13279..13788
                     /gene="crr"
                     /locus_tag="ACHY09_000130"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS3883154.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(13830..14681)
                     /gene="pdxK"
                     /locus_tag="ACHY09_000131"
                     /gene_synonym="thiJ"
     CDS             complement(13830..14681)
                     /gene="pdxK"
                     /locus_tag="ACHY09_000131"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFS3883155.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            14786..15154
                     /gene="yfeK"
                     /locus_tag="ACHY09_000132"
     CDS             14786..15154
                     /gene="yfeK"
                     /locus_tag="ACHY09_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFS3883156.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDDHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(15157..16068)
                     /gene="cysM"
                     /locus_tag="ACHY09_000133"
     CDS             complement(15157..16068)
                     /gene="cysM"
                     /locus_tag="ACHY09_000133"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFS3883157.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MLAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPFAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(16202..17299)
                     /gene="cysA"
                     /locus_tag="ACHY09_000134"
     CDS             complement(16202..17299)
                     /gene="cysA"
                     /locus_tag="ACHY09_000134"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFS3883158.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(17289..18164)
                     /gene="cysW"
                     /locus_tag="ACHY09_000135"
     CDS             complement(17289..18164)
                     /gene="cysW"
                     /locus_tag="ACHY09_000135"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFS3883159.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(18164..18997)
                     /gene="cysT"
                     /locus_tag="ACHY09_000136"
     CDS             complement(18164..18997)
                     /gene="cysT"
                     /locus_tag="ACHY09_000136"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFS3883160.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(18997..20013)
                     /gene="cysP"
                     /locus_tag="ACHY09_000137"
     CDS             complement(18997..20013)
                     /gene="cysP"
                     /locus_tag="ACHY09_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFS3883161.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(20171..20962)
                     /gene="ucpA"
                     /locus_tag="ACHY09_000138"
     CDS             complement(20171..20962)
                     /gene="ucpA"
                     /locus_tag="ACHY09_000138"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFS3883162.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(21091..21948)
                     /gene="murR"
                     /locus_tag="ACHY09_000139"
     CDS             complement(21091..21948)
                     /gene="murR"
                     /locus_tag="ACHY09_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFS3883163.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKEQALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSELVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            22112..23008
                     /gene="murQ"
                     /locus_tag="ACHY09_000140"
     CDS             22112..23008
                     /gene="murQ"
                     /locus_tag="ACHY09_000140"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS3883164.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSQEGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGPEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23012..24436
                     /gene="murP"
                     /locus_tag="ACHY09_000141"
     CDS             23012..24436
                     /gene="murP"
                     /locus_tag="ACHY09_000141"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFS3883165.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            24441..25742
                     /gene="pbp4b"
                     /locus_tag="ACHY09_000142"
     CDS             24441..25742
                     /gene="pbp4b"
                     /locus_tag="ACHY09_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFS3883166.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSALMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDAIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPCSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSDTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(25800..26699)
                     /gene="yfeX"
                     /locus_tag="ACHY09_000143"
     CDS             complement(25800..26699)
                     /gene="yfeX"
                     /locus_tag="ACHY09_000143"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFS3883167.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSIHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(26795..27370)
                     /gene="yfeY"
                     /locus_tag="ACHY09_000144"
     CDS             complement(26795..27370)
                     /gene="yfeY"
                     /locus_tag="ACHY09_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFS3883168.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27431..27880)
                     /gene="yfeZ"
                     /locus_tag="ACHY09_000145"
     CDS             complement(27431..27880)
                     /gene="yfeZ"
                     /locus_tag="ACHY09_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HFS3883169.1"
                     /translation="MKSTEFHPVHYDAYGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(27867..28292)
                     /gene="ypeA"
                     /locus_tag="ACHY09_000146"
     CDS             complement(27867..28292)
                     /gene="ypeA"
                     /locus_tag="ACHY09_000146"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFS3883170.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28506..29375
                     /gene="amiA"
                     /locus_tag="ACHY09_000147"
     CDS             28506..29375
                     /gene="amiA"
                     /locus_tag="ACHY09_000147"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFS3883171.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            29379..30278
                     /gene="hemF"
                     /locus_tag="ACHY09_000148"
     CDS             29379..30278
                     /gene="hemF"
                     /locus_tag="ACHY09_000148"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFS3883172.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGTEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEDDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPEDGSPEAALSEFIKVRDWV"
     gene            complement(30212..31336)
                     /gene="eutR"
                     /locus_tag="ACHY09_000149"
     CDS             complement(30212..31336)
                     /gene="eutR"
                     /locus_tag="ACHY09_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFS3883173.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSSG"
     gene            complement(31382..31882)
                     /gene="eutK"
                     /locus_tag="ACHY09_000150"
     CDS             complement(31382..31882)
                     /gene="eutK"
                     /locus_tag="ACHY09_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFS3883174.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(31895..32554)
                     /gene="eutL"
                     /locus_tag="ACHY09_000151"
     CDS             complement(31895..32554)
                     /gene="eutL"
                     /locus_tag="ACHY09_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFS3883175.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(32564..33451)
                     /gene="eutC"
                     /locus_tag="ACHY09_000152"
     CDS             complement(32564..33451)
                     /gene="eutC"
                     /locus_tag="ACHY09_000152"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFS3883176.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCTTPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(33472..34833)
                     /gene="eutB"
                     /locus_tag="ACHY09_000153"
     CDS             complement(33472..34833)
                     /gene="eutB"
                     /locus_tag="ACHY09_000153"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFS3883177.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(34845..36248)
                     /gene="eutA"
                     /locus_tag="ACHY09_000154"
     CDS             complement(34845..36248)
                     /gene="eutA"
                     /locus_tag="ACHY09_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFS3883178.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTEARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPVGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDEKDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(36245..37471)
                     /gene="eutH"
                     /locus_tag="ACHY09_000155"
     CDS             complement(36245..37471)
                     /gene="eutH"
                     /locus_tag="ACHY09_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFS3883179.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(37688..38875)
                     /gene="eutG"
                     /locus_tag="ACHY09_000156"
     CDS             complement(37688..38875)
                     /gene="eutG"
                     /locus_tag="ACHY09_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFS3883180.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSLVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(38865..39701)
                     /gene="eutJ"
                     /locus_tag="ACHY09_000157"
     CDS             complement(38865..39701)
                     /gene="eutJ"
                     /locus_tag="ACHY09_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFS3883181.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKTEGIY
                     AK"
     gene            complement(39712..41115)
                     /gene="eutE"
                     /locus_tag="ACHY09_000158"
     CDS             complement(39712..41115)
                     /gene="eutE"
                     /locus_tag="ACHY09_000158"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFS3883182.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(41127..41414)
                     /gene="eutN"
                     /locus_tag="ACHY09_000159"
     CDS             complement(41127..41414)
                     /gene="eutN"
                     /locus_tag="ACHY09_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFS3883183.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(41521..41814)
                     /gene="eutM"
                     /locus_tag="ACHY09_000160"
     CDS             complement(41521..41814)
                     /gene="eutM"
                     /locus_tag="ACHY09_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFS3883184.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(41853..42869)
                     /gene="pta"
                     /locus_tag="ACHY09_000161"
     CDS             complement(41853..42869)
                     /gene="pta"
                     /locus_tag="ACHY09_000161"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS3883185.1"
                     /translation="MIIERCRKLALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFALRWLARAGEKTPPDALDKLTD
                     PLMFAAAMVSAGTADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVQRQTEVNRESSLQ
                     TLVE"
     gene            complement(42866..43669)
                     /gene="eutT"
                     /locus_tag="ACHY09_000162"
     CDS             complement(42866..43669)
                     /gene="eutT"
                     /locus_tag="ACHY09_000162"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFS3883186.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQSLTVKQIQQRLGETQ"
     gene            complement(43666..44367)
                     /gene="eutQ"
                     /locus_tag="ACHY09_000163"
     CDS             complement(43666..44367)
                     /gene="eutQ"
                     /locus_tag="ACHY09_000163"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFS3883187.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(44342..44821)
                     /gene="eutP"
                     /locus_tag="ACHY09_000164"
     CDS             complement(44342..44821)
                     /gene="eutP"
                     /locus_tag="ACHY09_000164"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFS3883188.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(44834..45169)
                     /gene="eutS"
                     /locus_tag="ACHY09_000165"
     CDS             complement(44834..45169)
                     /gene="eutS"
                     /locus_tag="ACHY09_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFS3883189.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(45462..47741)
                     /gene="maeB"
                     /locus_tag="ACHY09_000166"
     CDS             complement(45462..47741)
                     /gene="maeB"
                     /locus_tag="ACHY09_000166"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFS3883190.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYRGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            48030..48980
                     /gene="tal"
                     /locus_tag="ACHY09_000167"
     CDS             48030..48980
                     /gene="tal"
                     /locus_tag="ACHY09_000167"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFS3883191.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            49000..51003
                     /gene="tkt"
                     /locus_tag="ACHY09_000168"
     CDS             49000..51003
                     /gene="tkt"
                     /locus_tag="ACHY09_000168"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS3883192.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKYAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(51106..52149)
                     /gene="ypfG"
                     /locus_tag="ACHY09_000169"
     CDS             complement(51106..52149)
                     /gene="ypfG"
                     /locus_tag="ACHY09_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFS3883193.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(52275..52850)
                     /gene="nudK"
                     /locus_tag="ACHY09_000170"
     CDS             complement(52275..52850)
                     /gene="nudK"
                     /locus_tag="ACHY09_000170"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFS3883194.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     NKALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(52918..54897)
                     /gene="aegA"
                     /locus_tag="ACHY09_000171"
     CDS             complement(52918..54897)
                     /gene="aegA"
                     /locus_tag="ACHY09_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFS3883195.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QVQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            55103..56803
                     /gene="narQ"
                     /locus_tag="ACHY09_000172"
     CDS             55103..56803
                     /gene="narQ"
                     /locus_tag="ACHY09_000172"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFS3883196.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            56967..60080
                     /gene="acrD"
                     /locus_tag="ACHY09_000173"
     CDS             56967..60080
                     /gene="acrD"
                     /locus_tag="ACHY09_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFS3883197.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAQLVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(60179..60238)
                     /gene="ypfM"
                     /locus_tag="ACHY09_000174"
     CDS             complement(60179..60238)
                     /gene="ypfM"
                     /locus_tag="ACHY09_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFS3883198.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        14186 a        16668 c        15410 g        14136 t
ORIGIN      
        1 atggataata tacccggtaa tacatttggt acaggaatag gcaagcgcac gattgtaaac
       61 aaagcaccaa tgccaaaacc aacgccagaa gaaaataaaa tatctttcac taattaacct
      121 ttatcataaa agcagcccca aggaacagag ctgcgaatcc ttttaatgtg taactgaacg
      181 atgcgttatc ttttcagggt catggttata tttaaaccca aagaaaaata tcactgcgag
      241 gaaaagagca taccctgcaa acatcagcca aatggtatgc caatctttta cgccatccac
      301 cgagaagtaa tccactgcca taccgctcag aatcgagcca acccatgcgc cgacaccatt
      361 taccatggtc ataaaaaggc cctgggcgct ggcacgaatg ctggaatcaa cttcctgttc
      421 gacaaatacc gaaccagaaa tattgaagaa atcgaatgca cagccataaa caatcatcga
      481 cagcagcagc aaaataaacc cggttggtga tgggtcgcca taggcaaaga agccaaagcg
      541 cagcgtccac gccaccatac tcatcagcat gacggtttta ataccgaaac gcttcaaaaa
      601 gaacggtatc gtcagaatga aaccgacttc agccatctgt gaaactgaca gtaaaatgga
      661 cggatatttc accacgaagc tatcagcaaa ctcaggatta cgggcgaaat catgcaggaa
      721 cgggttgcca aaaacgttgg taatttgcag caccgcgccc agcatcatgg cgaagaggaa
      781 aaagatagcc atgcgtggat ttttaaacag cacgaaggca tccagaccca gcttgctggc
      841 aagcgatgta gtcgcttttt tctccgcaac cggaatcttc ggcaaagtca gcgcataagc
      901 cgacagcagc aatgacgcac cggacgcgat atacagctgc agactactca attccagatg
      961 cagcaggctt actgcccaca tggcgacaat gaaccccacc gtaccaaaaa cgcgaatggg
     1021 cgggaaagcg gtcaccgggt caagccctgc ctgggcaaga caggaataag agacgctgtt
     1081 cgataacgca atagtcggca taaacgccat cgcattgact aacatcaccc aaaacatcat
     1141 atccggatca gttacggatg ccgcataaaa aagtacgccc gcacacacca ggtgacacag
     1201 catgtatgca cgttctgcac gcagccattt gtctgcgatg atccccatta tacccggcat
     1261 aataatcgcg gcgatccctt tggaactgta aaccatgcca acattagcgc cggtgaaatg
     1321 aagggtatta atcatgtaag aaccgagggt aaccagccag ctcccccaga taaaatattg
     1381 caaaaaggac attaccttta agcgcaaagc gatgctcata atcgtttcct tgtcgcatca
     1441 tatggcctca ctcagtaagg ccgtttattg ttgttttact caggcaattt tgcgcagaaa
     1501 gccgcaaata agattaataa agttttgctt tgagagttcc gctgctgcca gcgtttgggc
     1561 atgagaaagc ttcacgtcac tcagaccttc cgccatgttg gtaatcgcag agaccgcaac
     1621 gactttaagt tcgcaatggc gagctgaaat aacctcaggc accacagaca taccaacaac
     1681 atccccacca ataatttgca tcatacgaat ttccgccgca gtctcgaaat tcggccccgg
     1741 atacgagacg aacacgccct ccgtcagagg gaacccctct tctttcgcca ctttttgtaa
     1801 cagtgcgcgg tattccgcat cgtaggcatt cgccagcgag aagaagcgct caccaaaacg
     1861 ttcatcatta agacccacca tcggcgttcc cggcatggtg ttgatgtgat ctttcaatgc
     1921 gaccagactg ccggccccca cttcaggacg cagtgagcca gccgcattgg tgcagaacag
     1981 caactcgcag cccagcaact taaaggtacg gattgcatcc gtcatgatgg tcatcccacg
     2041 accttcgtag aaatgtccgc gacctttcat gcacgccact ggcaccccct ggagataccc
     2101 cagcaccagc tcacccgcat gaccgtgtac ggtacttacc gggaacccag gcagcttttc
     2161 gtaggaaatt gcgaccgcgt tctcaatctg atcggccagc gcgcccagcc cggaacctaa
     2221 aataaaggcc actcgtggcg tgaaatcagg tttataagtc ttgataatat ctacgcaata
     2281 cagtgggtta tgagaaaact gagcctgaga catatatatc ctttttttgt agggtggaat
     2341 ctaacgcctt tttataacga gattgcccta cctcatacca atacactttt ttcatgtatc
     2401 cgatagttat tcgatatcga tatatttata ttttttcact attaataggc tgaaaaaact
     2461 gtattggtac tctcctttcc cttgcattaa caatatgccg cttttaatca accttttatt
     2521 aagcaatcaa ctaataacga tcggcatagg gaattatgct atgaaaaaac atctttttac
     2581 tctgacactc tcttctgtat tggcgatacc agcagtatcc catgcagaat ttaaaggcgg
     2641 ttttgctgat ataggtattc attatcttga ctggactagc agaaccactg agaaatcgtc
     2701 gaccaaatca cacaaagatg attttggata tctggaactg gaaggcggcg ctaactttag
     2761 ttggggagaa atgtacgggt tcttcgactg ggaaaacttt tataatgatc gtcatgataa
     2821 accgggtagc gagcagcgtt atacctttaa aaataccaac cgtatttacc tgggtgacac
     2881 cggatttaat ctctacttgc atgcgtatgg cacctacggt tctgcgaatc gcgtgaattt
     2941 ccatgacgat atgttccttt acggtatcgg ctacaatttc accggtagcg gttggtggtt
     3001 caaaccgttc tttgcaaaac gctatacaga ccaaacctat tataccggcg ataacggtta
     3061 tgtcgccggt tgggttgcag gttacagctt tatgctgggc agtgagaaat tcaccctcac
     3121 caactggaac gagtacgagt ttgatcgtga tgccacctac gcggcaggta atggcggcaa
     3181 agaaggtctg aacggtgccg tcgcactttg gtggaatgca acttcacaca tcaccacagg
     3241 aattcagtat cgttatgcgg atgacaaact gggcgaagat ttttaccagg atgcaataat
     3301 ctactccatc aaatttaatt tctgattgat tactgccggg gctcccggca gtatgaatag
     3361 ctctatatct gatacagcgg atccccttcc cgcccagcca ttagcgcacg gccctggcct
     3421 cgtaccactt cccataacgc ttgtgcggcg gtggaaagtg aacggttctt cctgcgcacc
     3481 agcatcaact ggcgttccac caccggcgta atgcgcttta ccaccagcgg gctaccttca
     3541 ggtaacggca gcgccagtgc gggaagaata ctaatgccga tgcctgcggc taccatcggg
     3601 aacagcgtcg ccggatgacc aatctcctgc acaatattcg cctgaatacc gttgcgcgcc
     3661 agtgctgcat caatcagcgg tcggctgcct gacgcgtaat cctgcaacac caatttcgct
     3721 ccctgtaatg cctgccaggg tacataatct tccgcggcca aagcgctatc gcgatggcaa
     3781 agcaagaaga aaggctcgga aagaatcgct tcacattgca gatcgctcac tgggcctgga
     3841 tcgatgacga tgccaaaatc gacatctccc tggcgaatac tttccatcac ccactgctgc
     3901 ggacgatcgt gcaggataaa ctgaatatct ggatagcggc gatggctttc agcaatgcat
     3961 tgcggaataa gatgcgcgga aatggtctgg ctggcagcga cccgaacttt tccgctcagt
     4021 tgttgcccca tacgcccggt atcgcgcaac gtgctgttaa gttcatccag cagtcgctca
     4081 agacgcaaag ccaactgctg ccctgcatct gtaagcacca cttcacgcgt ggttctgtcc
     4141 agcaggcgaa caccagtatg attttccagc tccttcacac tgtgactcac tgccgactgg
     4201 ctcaggccga tacgctctcc tgcacgacta aaacttttct cctgcgctac tgtgacgaaa
     4261 acctttaatt gttttaaaga ataattcatc ttttttcttc atgaatggat gcaataaatc
     4321 aattttattt ctcaaactgg aagaagcaca atagaaccat cgatcatctg gagtctttat
     4381 gaaacttttt cgtatcctcg atcctttcac cttaaccctg atcacggtgg tgttgctggc
     4441 ctctttcttt ccggccagag gcgatttcgt ccccttcttt gaaaatctga ccactgcagc
     4501 tattgccctg ctgttcttta tgcacggcgc gaagttgtcg cgtgaggcga ttattgctgg
     4561 cggtgggcac tggcgactgc atttgtgggt aatgtgcagc accttcgtat tgtttccgat
     4621 tctgggcgtg ctgtttgcct ggtggaaacc ggtaaatgtc gacccgatgc tctactccgg
     4681 ttttctctac ttgtgcattc tccccgctac cgtgcagtct gcaatcgcct tcacgtcaat
     4741 ggcgggcggt aacgttgcgg cggcggtttg ttctgcgtca gcatccagcc tgcttgggat
     4801 tttcctttca ccattgctgg ttggtctggt gatgaatgtt cacggtgcgg gaggcagcct
     4861 tgagcaggtc ggtaaaatta tgctgcaatt gctactgccg tttgtgttgg ggcatctttc
     4921 ccggccgtgg attggtgact gggtgtcgcg caataaaaaa tggattgcga aaactgacca
     4981 gacgtccatt ctgttggtgg tttatacggc attcagcgaa gccgtcgtta acggtatctg
     5041 gcacaaagtt ggctggggat cattgctgtt tatcgtggtg gtcagttgcg ttcttctggc
     5101 tatcgtgatt gtagttaacg tctttatggc acgccgactg ggcttcaata aggcagatga
     5161 aattactatc gtcttttgtg gttcgaaaaa gagtctggca aatggcatcc cgatggcaaa
     5221 cattctgttc cccacatcgg tgatcggtat gatggtgctg cccctgatga ttttccatca
     5281 gatccaattg atggtctgtg cggtgctggc gcgtcgatac aaacgccaga ccgaacagtt
     5341 acaggcgcag caggaaagca gcgccgataa agcttaaagc ggacgcttca ggggctggac
     5401 cagttgcgtc agcccctcgg ttttaatcag cagcgtgatt tgcattagct caccgagctt
     5461 ccctgccggg aactcatctt tgcgggcaaa ccacagcaga tactcctccg gcaggtcgat
     5521 taagcgacgc cctttgtact tgccaaacgg cattatcgta ttggctattt caatcagctg
     5581 ctctttttcc atctcagcta cccagcaaac gcagcatttc cgtttcgtcg atgacttcaa
     5641 tacccagttc ctgcgccttc gccagtttag atcctgcagc ttcacccgct atcaccagat
     5701 cggttttctt cgacacgctg cccgcgactt tcgcgcccag ttcgaccagt cgagctttag
     5761 cgtcatcacg cgacatctgg cttaagctgc ccgtaagcac cacggtttta ccagcaaacg
     5821 ggctgtcaat ctcttccgcg ttaatcacca ccggcgcagg ccagtgaacg ccttccgcca
     5881 acagctcgct gatgacattg cggttgcttt cttcggcaaa gaagttgtga acgtgggagg
     5941 caacgacaat gccaacatca ggcacctttt gcagctcttc aatcgaagcg gcttccagcg
     6001 cttccagcgt gccgaaatat gccgccagac ctgctgcggt ggcctcgccg acttcacgga
     6061 tgccaagtgc atagaggaag cgagcaaagg tggtttcttt cgctttttcc agcgcgttaa
     6121 ccacgttttg tgccgatttc ggccccatac gctccagtcc ggtcagtttg actgcggtga
     6181 gtttgaacag atccgccgga gtgtggacat attctttttc aaccagttga tcgatgattt
     6241 tgtcgcccat tccgtcaaca tccatcgcac ggcgggaaac aaagtgtttc agagactctt
     6301 tacgctgcgc accgcaaatc aggccaccgg tacagcgggc aaccgcttca ccttccacac
     6361 gctcgacgtc agaaccacat accggacaat gcgtcgggaa tacaacctca cgggtatctt
     6421 ccgggcgttc agaaagcacg acgttaacca cctgcgggat cacgtcgcca gcgcggcgaa
     6481 taaccacttt atcgccaatg cgtaaaccaa gacgttcgat ttcgtcagca ttatgcagtg
     6541 tagcgttact caccaacacg ccagcaacat ggacaggttc cagacgcgca acaggcgtaa
     6601 tcgcgccagt acgcccaacc tgaaactcga cgtcacgcac aaaggtcatc tgttcctgcg
     6661 ccgggaattt aaacgctacc gcccagcgcg gggcacgcgc gacaaagcca agctgctcct
     6721 gctgtgccag tgagttgacc ttaatcacca cgccgtcgat atcaaagccc agcgtcggac
     6781 ggtctttttc tacttcgtga taaaacgcca gcacttcttc cgccgattca caaagtgtta
     6841 cccgatcact gacaggtaat ccccacgctt taaattgcat taagcgtccc aaatgggtat
     6901 ccggcaattc accgccttcc agaacaccaa cgccatagca gaagaaagtg agcggccgct
     6961 tcgctgtaat acgcggatca agctgacgca gtgaaccagc tgccgcatta cgtgggttag
     7021 caaacacttt cccgcccgtg cgtcgcgcat cttcgttaat cttttcgaac cccgcctgcg
     7081 gcaggaacac ttcaccacgc acttccagac gcgccgggat attctctccg tgtagcttca
     7141 gcggaatggc gcgaatagta cgcacattag acgtgatatc ttccccggtg gtgccatcgc
     7201 cacgggtggc ggcactgact aaaacaccat tttcatacag aatactgacg gcaagaccat
     7261 ccagcttcag ctcacagcac caggtgactt tctcgttgct tttcagacgg tcctgcacac
     7321 gtttgttgaa agcaagaaag ctttcttcat caaaaacgtt atccagcgac agcattggca
     7381 cttcatggcg gatctggctg aaagccgcca gcggcgcagc acctacacgt tgggtaggcg
     7441 aatcaggcgt aatcagttct ggatgtttgg tttccagctc gcgcaattcg cgcatcagcc
     7501 tgtcgtattc agcgtcggga atttccggcg catccatcac atgataaaga tattcatgat
     7561 ggcgaagcgt cgttcgcagt tctgtcagtt gttgttcgat tgattccata tcgcaccatc
     7621 aatgctaaaa acccccgaca agcgggggtt cgaagaggag ttaatttgcc ttaagtgtat
     7681 caggcgttgg cgtctttgac ttcgcggatg atgtcctggt actcgcgcaa tttctgcgga
     7741 gtcatcatac ggcgctgatc gtcaagaaca acaccgccca cttcatcggc aatatgctgc
     7801 gcagattgca gcatcagctt gaagttctgc agctcgtcac cgtaagacgg tacctgcata
     7861 aagatagtga cacccggagt agtgaaatcc ttcatttcag gatcaaaggt tcccggtttc
     7921 accatattcg ccaggctgaa taacgccggg ccgctgccat ccgggctaag atgacgatgg
     7981 taaatattca tatcgccaaa aatgaagccc gcttgttgaa tgctgttaag aagcagttca
     8041 ccgtttagct cgctaccgtg atgcgccgcg acgttcatga taatcaccgc ttctttgcgc
     8101 ttcggtttat ccataactgg agccggttcc gctacaggct caggctgtgg tgccgctacg
     8161 ggttctgcag gctggaaagc ctgttgtgcc ggttgcggtg ctgaatgcac tggctgcggc
     8221 gctggcgcga cctgtggcga aactggctgc tgcaacggct gttcaggctg cggctgatag
     8281 gcaggctgct gcacgggttg ctgcaccagc tgcgctggac gcggagcatg ttgcggcggt
     8341 acctgcgctt caggcggctg ctggaccggt tgacgcggct gcgcagacgc ataaggcggt
     8401 tggtactggt gttgcggcga cggacgagca gcctcatgct cctgggcgtt agccggggca
     8461 tgattcacgc ggtgaactcg aacctcacca acgccctcat catcttcgac atcctcgtca
     8521 taagaatcgt cgtcacgttt tgacttcatt cgttttaatg gccgatcgcg gaacatagaa
     8581 gatcgttctt tacggctggt ccagaaacca tgtaccagta aagcgattat ggcgatcgcg
     8641 ccaacaatga ttaatatcag acgcaaatcc tgcatcatta tattctctgt tgttctaaca
     8701 ccttgccacc acggcaaaca tttactcact aagagtattt gtcgattacc tcaagtgcaa
     8761 gtgcactatt aactttcaca gcacaaagat agatgaaatc gtgctttttg ctgttttttc
     8821 gaacatatcc taactgtcca ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg
     8881 attggtcaaa aggagctcat cctgattatg gtttcatcat tcacatctgc cccacgcagc
     8941 ggtttttact attttgcgca aggctggaag ctcgtctcgc aacctgggat tcggcgtttc
     9001 gttattttac cgctgctggt caatattttg ttgatggggg gcgcattctg gtggctcttt
     9061 acgcaactcg atgtctggat cccgactctc atgagttatg ttccggactg gttacaatgg
     9121 ctgagctatc tgttgtggcc cctggctgtc atctctgtgc tgttagtatt tggttatttc
     9181 ttttcaacga ttgctaactg gattgccgct ccgttcaacg gtttattggc tgaacaactg
     9241 gaagcaagac tgactggcgc tacaccgcca gataccggaa ttttcggtat catgaaagat
     9301 gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt
     9361 gtattgctaa ttctttactt catccctggt attgggcaaa ccgtcgcgcc agtactgtgg
     9421 ttcctgttta gcgcctggat gttagccatc cagtattgcg attacccctt cgataaccac
     9481 aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag
     9541 tttggtgctt taaccagcct gtttacgatg atcccgttgc ttaatctgtt catcatgccc
     9601 gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg
     9661 tggcggtaac aatctaccgg ttattttgta aaccgtttgt gtgaaacagg ggtggcttat
     9721 gccgcccctt attccatctt gcatgtcatt atttcccttc tgcatataga tatgctaaat
     9781 ccttacttcc gcatattctc tgagcgggta tgctacctgt tgtatcccaa tttcatacag
     9841 ttaaggacag gccatgagta agatttttga agataactcg ctgactatcg gtcacacgcc
     9901 gctggttcgc ctgaatcgca tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa
     9961 ccccagcttc agcgttaagt gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg
    10021 cggcgtgctg aaaccaggcg ttgaactggt tgaaccaacc agcggtaata ccgggattgc
    10081 actggcctat gtcgctgccg ctcgcggtta caaactcacc ctgactatgc cagaaaccat
    10141 gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca aacctggtgc tgaccgaagg
    10201 tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa attgtcgcca gcaatccaga
    10261 gaaatacctg ctgctgcaac aattcagcaa tccggcaaac cctgaaattc acgaaaaaac
    10321 caccggtccg gagatctggg aagataccga cggtcaggtt gatgtattta ttgctggcgt
    10381 tgggactggc ggtacgctga ctggcgtcag ccgctacatt aaaggcacca aaggcaagac
    10441 cgatcttatc tctgtcgccg ttgagccaac cgattctcca gttatcgccc aggcgctggc
    10501 aggtgaagag attaaacctg gtccgcataa aattcagggt attggcgctg gttttatccc
    10561 ggctaacctt gatctcaagc tggtcgacaa agtcattggc atcaccaatg aagaagccat
    10621 ttctaccgca cgtcgtctga tggaagaaga aggcattctt gctggtatct cttctggagc
    10681 agctgttgct gcggcgttga aactacaaga agatgaaagc tttaccaata agaatattgt
    10741 ggttattctg ccgtcatcgg gtgagcgtta tttaagcact gcattgtttg ccgatctttt
    10801 cactgagaaa gaactgcaac agtaatgcca gcttgtaaaa aatgcgtaaa aaagcacctt
    10861 tttaggtgct tttttgtggc ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct
    10921 gtcggaactg gtatttaacc agactaatta ttttgatgcg cgaaattaat cgttacagga
    10981 aaagccaaag ctgaatcgat tttatgattt ggttcaattc ttcctttagc ggcataatgt
    11041 ttaatgacgt acgaaacgtc agcggtcaac acccgccagc aatggactgt attacgctct
    11101 tcgtgcgtcg cgtctgttaa aaactggcgc taacaataca ggctaaagtc gaaccgccag
    11161 gctagacttt agttccacaa cactaaacct ataagttggg gaaatacaat gttccagcaa
    11221 gaagttacca ttaccgctcc gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa
    11281 gaagctaagg gcttcacttc tgaaattact gtgacttcca acggcaaaag cgccagcgcg
    11341 aaaagcctgt ttaaactgca gactctgggc ctgactcaag gtaccgttgt gactatctcc
    11401 gcagaaggcg aagacgagca gaaagcggtt gaacatctgg ttaaactgat ggcggaactc
    11461 gagtaatttc ccgggttctt ttaaaaatca gtcacaagta aggtagggtt atgatttcag
    11521 gcattttagc atccccgggt atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa
    11581 ttgtcattga ccggaaaaaa atttctgccg accaggttga tcaggaagtt gaacgttttc
    11641 tgagcggtcg tgccaaggca tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa
    11701 cgttcggtga agaaaaagaa gccatctttg aagggcatat tatgctgctc gaagatgagg
    11761 agctggagca ggaaatcata gccctgatta aagataagca catgacagct gacgcagctg
    11821 ctcatgaagt tatcgaaggt caggcttctg ccctggaaga gctggatgat gaatacctga
    11881 aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc
    11941 tgaagattat cgacctgagc gccattcagg atgaagtcat tctggttgcc actgatctga
    12001 cgccgtccga aaccgcacag ctgaacctga agaaggtgct gggtttcatc accgacgcgg
    12061 gtggccgtac ttcccacacc tctatcatgg cgcgttctct ggaactgcct gctatcgtgg
    12121 gtaccggtag cgtcacctct caggtgaaaa atgacgacta tctgattctg gatgccgtaa
    12181 ataatcaggt ttacgtcaat ccaaccaacg aagttattga taaaatgcgc gctgttcagg
    12241 agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa agatctgcca gctattacgc
    12301 tggacggtca ccaggtagaa gtgtgcgcta acattggtac ggttcgtgac gttgaaggtg
    12361 cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc
    12421 gcgacgcgct gcccactgaa gaagaacagt ttgctgctta caaagcagtg gctgaagcgt
    12481 gtggctcgca ggcggttatc gttcgtacca tggacatcgg cggcgacaaa gagctgccat
    12541 acatgaactt cccgaaagaa gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga
    12601 tggaccgtaa agagatcctg cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg
    12661 gtaaattgcg cattatgttc ccgatgatca tctctgttga agaagtacgt gcactgcgca
    12721 aagagatcga aatctacaaa caggaactgc gcgacgaagg taaagcgttt gacgagtcaa
    12781 ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac aattgcacgt catttagcca
    12841 aagaagttga tttctttagt atcggcacca atgatttaac gcagtacact ctggcagttg
    12901 accgtggtaa tgatatgatt tcacaccttt accagccaat gtcaccgtcc gtgctgaact
    12961 tgatcaagca agttattgat gcttctcatg ctgaaggcaa atggactggc atgtgtggtg
    13021 agcttgcagg cgatgaacgt gctacacttc tgttgctggg gatgggtctg gacgaattct
    13081 ctatgagcgc catttctatc ccgcgcatta agaagattat ccgtaacacg aacttcgaag
    13141 atgcgaaggt gttagcagag caggctcttg ctcaaccgac aacggacgag ttaatgacgc
    13201 tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt
    13261 actgcttagg agaagatcat gggtttgttc gataaactga aatctctggt ttccgacgac
    13321 aagaaggata ccggaactat tgagatcatt gctccgctct ctggcgagat cgtcaatatc
    13381 gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg gtgatggcat tgctatcaaa
    13441 ccaactggaa acaaaatggt cgcgcctgta gacggcacca ttggtaaaat ctttgaaacc
    13501 aaccacgcgt tctctatcga atctgatagc ggcgttgaac tgttcgtcca cttcggtatc
    13561 gacaccgttg aactgaaagg cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg
    13621 aaagttggcg atactgtcat tgaatttgat ctgccgctgc tggaagagaa agccaagtct
    13681 accctgactc cggttgttat ctccaacatg gacgaaatca aagaactgat caaactgtcc
    13741 ggtagcgtga ctgtgggtga aaccccggtt atccgcatca agaagtaatt cttgccgcag
    13801 tgaaaaatgg cgcccatcgg cgccattttt tatgcttccg ccagcggcgg caaaatcaat
    13861 tcatcgctct catgctgctg ggtgtagcgc atcacttcca gtacgcgcaa ccccgcacgg
    13921 tgcaccgcat cgtttaacgc cttccctttc agcaatccac tgataagctg agcacaaaac
    13981 aggtcgccag tccctttcag gtcggttttt acccgtgaat gggaaatgac attcacgctg
    14041 tcggcactga ccaccaccac ctgcatctcc tgattttctt cattaccgga ggcgctggta
    14101 atcaccaccc attttaatgt gtctgaaagc agactttttg cggcagcaat ggcactgtcg
    14161 agatcgcggc aatttttacc ggtcaggatt tccagctcaa agatattggg ggtaattccc
    14221 tgcgccagcg gcagtaaata ttgtcgatac gcctcgggaa ggtcaggttt gacataaatt
    14281 ccgctatcaa tatcgccaat caccggatcg accatgatca ataggtcagg atggtctttg
    14341 cgtagcgcag tcagccactc ggcaaggatt ttgatttgcg atgccgttcc catatagccc
    14401 gtggttacag ctcgaagttg gcgcagcgca tcacgctcct gaagcgcacg caaatagccg
    14461 ctaaaccatt catccggaat cgcaccaccg tagaaagtgt cataatgcgg tgtattgctc
    14521 agcaataccg tcggcacggc aaagacattc aggccgttct gtttgatagc aggcaccgca
    14581 atgctgttgc ctacgctgcc gtacactacc tgcgactgca cggcgacgat atccgcctgc
    14641 aatgccctgc tcttatcgtt aaacaacaac aaactactca tttaattttt tctccttgcc
    14701 gatgatcctc atcgtattcc aaccgaaact ttacctgatt ctggcagtca aatcggctat
    14761 cacaaaacaa ggataaggta attcaatgaa gaaaatcatt tgtctggtca ttacgctgct
    14821 aatgacactc cccgtttacg cgaagttaac tgcccatgaa gaagcccgta tcaacgccat
    14881 gctggaggga ttagcacaga aaaaggattt gatatttgtg cgtaacggcg atgatcatac
    14941 ctgcgatgaa gcggtttccc acctgcgtct gaagctcggc aatacccgta accgcattga
    15001 cactgccgag cagtttattg ataaggtagc ctcgtcgtca tcgattaccg ggaagccgta
    15061 tatcgtgaag atgcccggta aaagcgatga gaacgcacaa ccttttttac atgcgctgat
    15121 tgcgcagacg gataaaacgg tgcctgcgca ataatcttaa atccccgccc cctggctaaa
    15181 atgctcttcc ccaaacaccc cggtagaaag gtagcgatcg ccgcgatcgc agatgatcgc
    15241 cacaaccacc gcgccggggt tagcttttgc cacgcgcagt gctccggcaa ccgcgccgcc
    15301 ggagctgacg ccacagaata ttccttcccg cactgccagt tcgcgcatgg tgttttccgc
    15361 atcgcgctga tgaatatcca gcacctcatc caccagcgaa gcgttgaaaa tccccggcag
    15421 atattccgca ggccagcggc gaatgccggg aatgctactg ccctcttccg gctgcaggcc
    15481 aacaatggtc accggtttgg attgttcgcg cataaagcgt gaaacgccgg tgatagtgcc
    15541 ggttgttccc atgctggaga caaaatgggt aatgcgcccg ccggtttgct gccagatttc
    15601 cggcccggtg gtggtgtaat gggcgaaggg attatcagga ttattgaact gatcgagcag
    15661 ctttccttca ccacgattgg ccatctccag cgccagatcg cgcgcgcctt ccatgccctg
    15721 ctctttggtg acaagaatca gttccgcacc gtatgccagc atcgctgcac ggcgctcctg
    15781 gctcatgttg tcgggcatca gcaatttcat gcgatagcct ttaagcgcgg caatcattgc
    15841 cagcgcaatg ccggtgttac cactggtggc ttcaatcagg acatcgcccg gtttgatttc
    15901 tccgcgcttt tccgcctcga cgatcatcga aagcgctgca cgatctttca ccgaacctgc
    15961 cgggttattg ccttccagtt tcaaccagac ttcactgccg ttatccggcg ccattcgctg
    16021 caacttcacc agaggcgtat tgccaattgt ttgttctaat gtactcacga tctctgtcca
    16081 tacgtggtgt tgcctgatgc gacgcttgcg cgtcttatca ggcctacagg ttacaaacct
    16141 tgccattaaa aagcccggat cgcgggaagc gcctccgggc gtttaacatt cactcaacct
    16201 atcaggcgct ttgtgcgaga gcaagttcct catcgcgggt ttcaatacgc tcgtcgccgt
    16261 tatacagccg cgcatgttgc agaccaacga ataaacgctc gccacgctgc ggggcatcgt
    16321 cgccatgcat cacaaccgtc agcggttcgt tgtaccaccc cagcggctgc acgactaatt
    16381 gggtgtagtg gcctttcggg ctggcttcca gtacctgtac cggcagcggc gaatcgaggc
    16441 tggtacggcg gctgatatcc acttcccacg gacgcaggaa gagatccacc ggcccctgat
    16501 acgcaggtgt atagcccagc ggccagcgat gtgcgccaac atggaactgc ccgccgcgaa
    16561 tggttccctg caggcggttc acttcgccca taaattcgag tacaaaacgg gtcgctggtt
    16621 cgcgccatac ctggtccggt gcatcagcct gctcgatatt gccctggctc atcaccacta
    16681 cacgatcggc tacttcggtc gcttcttcct ggtcatgggt cacaaacacg ctggtgaatt
    16741 tcagctcttc atgtaactga cgcagccagc gacgcaactc tttacgcacc tgcgcatcca
    16801 gcgcgccaaa cggttcatca agcagcagaa tttgcggttc tacagccagc gcgcgcgcca
    16861 gtgccacacg ctgtttctga ccgccggaaa gctgtgctgg ataacgatcc gccagatgcg
    16921 caagctgcac catctccagc aattttgtca ctttcgcttt gatggctgcg gcattcgggc
    16981 gctcgcgacg cggcagcacc gtcaggccga aagcgatatt gtcgaacacc gtcatatggc
    17041 ggaacagcgc atagtgctgg aacacgaaac cgactttacg atcgcgcgca tgcaggcggc
    17101 tgacgtcggt gccgtggaag cgaatatgcc cgctggtttg atgctccagc ccggcgataa
    17161 tgcgcagcag cgtggttttc ccggaaccgg acggccccag caacgcgacc atctgaccag
    17221 aaggaatatc cagtgagata tcgttcagca cctgggtgcg accaaaagac ttcttaatat
    17281 tggcaatctc aatgctcatg atgttcctcc tgctgcgcgc gtttttcctg attctccaga
    17341 cgccactgca acatactctt taaaaacagg gtgataatcg ccatcagcgt caacaacgcg
    17401 gcagcggtaa aggagccgac ggtgttgtag tcttgctcca gcaattcaat ctgtaacggc
    17461 agcgaaagtg tttcgccgcg aatcgagccg gaaaccaccg acaccgcacc aaactcgcca
    17521 atcgcgcggg cgttggtcag caccacgcca taaagcagcg cccagcggat gttcggcagc
    17581 gtgacgcggc gaaacatctg ccagccagac gcgccaagca aaatcgccgc ttcgtcttcc
    17641 tggctgccct ggcttaacat caccggcacc agctcacgca ccacaaacgg acacgtcacg
    17701 aagatggtga ccagcaccat tcccggccag gagaacataa tttgcagatt atgttcatcc
    17761 agccagccgc ccagcgggcc gttagagccg tagaacagca gatacaccag accagcaacc
    17821 accggcgaca cggcaaacgg aatgtccaac agcgtcagca atagctggcg tccggggaag
    17881 ttaaagcgcg tcaccagcca ggccagcaga ataccgaaca ccaggtttac cggtacggca
    17941 atcagcgcga tcatcaccgt cagccagatg gcgtgcagca tgtccggatc ggccagattc
    18001 tgtaaaaccg gcatcagccc tttgctgaac gcctgtacga agatataaat catcggcacc
    18061 agcaggatga acgccgatac cagcatcccg atgccaatca gaaaccattt gccccagtta
    18121 atcgggcgcg cgtcataacg cttcaactgt gtcacttccg ccattaatga cctaccacac
    18181 gccgaccaaa gcgactttgc agagtgttaa ttgagaacag cagcagcagc gatgccgcga
    18241 ggatcaccga ggcaatcgcg ctcgccgccg ggtaatcaaa ctcctgtaag cgaacaaaga
    18301 tcatcagcga cgtcacttct gttttccagg cgatattccc ggcgataaaa atcaccgcgc
    18361 caaactcacc gaggctgcgg gtaaacgaca gcgccacacc cgccaccagc gccggagata
    18421 gttccggcag caccactttg cgaaaactct gccagcgtgt tgcgccgagc gtttctgccg
    18481 cctcttcata ttccgggcct aactcttcca gcaccggctg cacggtgcgc accacaaacg
    18541 gaatgctggt aaaggccatc gccaccgcaa tccccagcca ggtataggtg actttgatat
    18601 caaacttcgc cagccattca ccgtaaaaac cgttcacgga aaagagcgag gccagcgtta
    18661 aaccggcgac tgccgttggc agcgcaaagg gtaaatccat cagcgcatca agcagcgtgc
    18721 gacccggaaa gcgatagcgg gttaagatcc acgccatcag cagaccgaaa acgccgttaa
    18781 aaatcgatgc cacaaacgcc gacagcagcg tcactttata ggctgcgacc acctgcgggt
    18841 tggtgatcac ctcccagtac tgcgcccagc tcatctgggc cagttgcatc accagcgcgg
    18901 agagcggcag cagcaaaatc aggcacacaa acagcagact ggtgccgagg cttaaggtaa
    18961 agcccggcag cacgcgtctt gaagaaactg caaacattac ttacgccccg ccgctaacag
    19021 cttgtctaac tcgccgccgc tggtgaagtg ggttttcatc acttccggcc aggagccaaa
    19081 tttatcttcc acgcggaaca gctcggtctg cgggaattta tctttcagtt tgcccatgac
    19141 ttccgggtta tttacgcggt agtaatagtc ggtgatgatg gtttgcgcct gcgggctgta
    19201 gagccagttc aggtaggctt ttgccgcttt ttccgtgccg ttggcctgta cgtttttatc
    19261 cacccacgcc accgggaatt ccgccagaat gttggttttc ggaatcacca cttcaaagcc
    19321 ctgcgcttca tactgtttac ggatgttgtt cacttccgac tcgaagctga tcagcacatc
    19381 gcccaggccg cgctcggcaa aagtggtggt cgcgccacga ccgccagtat cgaacacttc
    19441 aacgtttttc aggaactggg tcataaactg ttcggttttg gctttatcac caccgtcggc
    19501 tttgtccgct gcgccccatg ccgcaaggta ggtataacgc gcgttacccg acgttttcgg
    19561 gttcgggaaa atcagcttca cgtcagagcg taccaggtcg ttccagtcgt ggatattctt
    19621 tgggttgccc ttacgcacca ggaagcccat ggtggagtag aacggcgagc tattattcgg
    19681 caggcgcgac tgccagtccg ctgggatcag cttgccttta tcgtgcagga tttgtacgtc
    19741 ggtcacctgg ttataagtga caacgtcggc ttttaaaccc tgcaaaattg ccagcgcctg
    19801 ttttgatgat ccggcatgag attgtttaat cgtcagtttg tcgccaccgt tatcttttgc
    19861 ccattgttgc tcaaacggcg ggttcagggc ggcaaacagc tcgcgggaga cgtcataaga
    19921 actgttcagc agttccgttg cctgtacatg gcccgccagt agcagagaag caaccagcac
    19981 gagtgagttc tttttcagta agttaacggc cattgcgcac ccttataaat ttaatgactt
    20041 tctaatagcc atcatattta taacggacgt gaaaggagta acggttttat ataccgtttg
    20101 gtgatttgga agttgaaaag ggaataagac gaaatcctcc ccacaaatgc agggaggaaa
    20161 cagaggtgaa tcagatgccg acgctaaccg tctccggcag tgtgctgccg ccatcaatca
    20221 cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc gccagttcgc
    20281 cgacttccag cggatcggcg aggcgacgca tcgggattgc tttcgccatt tcagtcagca
    20341 ccgattctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt ggcgtgcgaa
    20401 cgtatccagg gcagatggcg ttaacgcgaa taccagactg cgcgtactcc accgccagcg
    20461 atttagtcag gccaacaatc gccgctttcg ttaaggcgta cgccgtttcg cccgggtctg
    20521 ccaccatatc gccagtgact gaagacatca tcacaatccg gccatctttg cgggcaatca
    20581 tctccggcag caccgccttc gtgacgttcc atacgccttt tatattgata tcaatatgga
    20641 aatcgcgatc ttcatcgctc atatcaagga aactgcccag acgacaaacg cctgcgttat
    20701 tcaccagaat atcaatgcgc ccttcttttt ccttagcgcg cttaatagct gcggcgaccg
    20761 acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacgg ccacacagtt
    20821 cgtccgccag cttttcgatc tcaggggaga tatccagcaa gattaggttt gcgccatgac
    20881 gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg
    20941 tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc gttacactcc
    21001 attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca ctaattcagg
    21061 aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgcgtta gctcactgct
    21121 gcgctcaatc attttcaatg actccacatc attgagttgc acaaggccaa caaacagtaa
    21181 atcggtaaca gaattttgcg ccgtgcgggt ggacatcgac gagctgcgcc attcggtttc
    21241 gccagaaacg gtatcgaggg tgtaatgcgc cagccgccgc agcggggaat cggttaatga
    21301 ggtaatggca atcaccgttg ccccctgctt tcgcgctgcc tcggcgcaca agacaatctc
    21361 ttttttactg ccactgtaag agatagcgat ttgtacatcg ccttttttta gcgcctgaga
    21421 aacggtggct tgtacgtggg tgtcggcctc acaggccacg cgataaccaa ttttcatcag
    21481 tttgaacgac agatcgcgcc ccaccagctc cgacccgccc aggcccgtta tctggataaa
    21541 cggtgcttta ctgatgacct caatgatttt ttgcagccgc gcgtagtcaa acaacgcgca
    21601 ggtttgctcc agcgcctgct ctttttcgcg attcagttta cgggcaatca cttccagcga
    21661 atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct
    21721 ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt
    21781 ctgggcaaac ttcacaatac tcgactggct gatgccgagc tgtttcgcca tcttgcgcga
    21841 agagacggat ttcagctcac tcacgttggc ccgcagaaag tcagcaattt tttgctcgtt
    21901 cccggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc
    21961 tgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcgagtgt
    22021 agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat
    22081 tatttgatcg taaatagtaa ggtcaccacc gatgcaactt gaaaagatga ttaccgaagg
    22141 ctcgaacgcc gcttcggctg aaattgaccg cgtatcaacg ctggaaatgt gccggattat
    22201 caacgatgaa gataaaaccg taccactcgc cgttgagcgc gtactgccgg atatcgccgc
    22261 agcgatcgat gttatccacg cccaggtcag cggcggcggt cgtctgattt atctcggtgc
    22321 gggaacatca ggtcgtctgg ggattctgga tgccagcgaa tgtccgccga cctacggcgt
    22381 gaaaccgggt ctggtggttg gattaattgc tggcggcgaa tatgccattc agcacgcggt
    22441 ggaaggcgcg gaagatagcc aggaaggcgg tgtgaatgat ctgaaaaaca tcggattaac
    22501 cgcgcaggat gtggtggttg gtattgccgc cagtggtcgc acgccgtatg tgattgccgg
    22561 actggaatac gcgcgccagc tcggctgccg cacagtggga atttcctgta atccagggag
    22621 cgcggtttcc accaccgctg agtttgctat tacgccgatc gttggtcccg aggtggttac
    22681 cggttcttca cgaatgaaag caggcacagc gcagaaactg gtgctcaata tgctctccac
    22741 cgggctgatg attaaatccg gcaaagtgtt cggcaaccta atggtcgatg tggtcgccac
    22801 caacgaaaaa ttgcatgtgc gccaggtcaa tatcgtcaaa aacgctaccg gatgtagcgc
    22861 agagcaagcg gaagcagcgt tgattgcctg cgagcgcaac tgtaaaacgg ccattgtgat
    22921 ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt
    22981 tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct
    23041 gaacaccatt cttacccggg tcggtggacc gggaaatatc gccagttgtg gtaactgtat
    23101 gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct
    23161 ggaaggcgtg aagggcgtca ttttaaccag cgaccaggtg caggtcgttt ttgggcctgg
    23221 taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggagaagcac cggtacagga
    23281 tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt
    23341 gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc
    23401 cgctggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga
    23461 tgctcagggc acgctacccg atgcgctgaa ttttatgaag gtgttcagca aaggcctgtt
    23521 caccttcctg gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt
    23581 caatggcgca attatcgccg cgctcttttt gctcggttat aacccaactg caaccaccgg
    23641 ttactacgcc ggatttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat
    23701 cggcgtgttg attgccgcgt gggcctgcgc ccgcatcgaa ggcatggtgc gccgctttat
    23761 gccggacgat ctcgacatgc tgttgacctc gttaatcacc ctgctaatca ccgctacgct
    23821 tgcgtacctg atcattatgc cgctgggcgg ctggctgttt gaaggcatgt cgtggctgtt
    23881 tatgcacctg aacagtaatc cgctcggttg tgcagtatta gccgggctgt tcctgatcgc
    23941 cgtggtgttt ggcgtgcatc agggctttat tcccgtttac ctcgcgttaa tggacagtca
    24001 gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc
    24061 gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagccagg tgcgcggggc
    24121 gattattccc ggtctgttgg gcgttggcga accgctgatt tacggcgtca ccctgccccg
    24181 catgaaaccg tttgttaccg cctgtttagg tggcgcggcg ggtggtttgt ttatcgggct
    24241 gatagcctgg tggggcctgc cgatgggctt aaacagtgcc tttgggccgt ctggtctggt
    24301 ggcgctgccg ctgatgacat cagcacaagg tatccttccg gcaatggcgg tttatgcagg
    24361 cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa
    24421 cgtcaatctg gactgataat atgaaacgga caatgctcta tctttctctg ctggctgtca
    24481 gctgtagcgt cagcgccgca aaataccctg ttctgacaga aagttcgcca gagaaagcag
    24541 ggtttaacgt cgaacggctt aatcagatgg atcgctggat aagccagcaa gtggacgccg
    24601 gttatcccgg cgtaaacctg ctgattataa aagataatca gattgtttat cgcaaagcct
    24661 ggggcgcggc gaaaaagtac gatggcagcg cgttaatggc gcagcctgtc aaagccacca
    24721 ccgggacgct gtatgacctg gcttccaata ccaagatgta cgccactaac ttcgccctgc
    24781 aaaagctgat gagcgaaggc aaactgcatc ctgacgatcg gattgcgaaa tatattccag
    24841 ggtttgccga cagcccgaac gatgcgatca aaggcaaaaa cacgctgcgg atttctgatc
    24901 tgctgcatca cagcggcggt ttcccggcag atccgcaata cccgaataaa gcggtcgcgg
    24961 gcgcgttata ttcccaggat aaaggccaga cgctggagat gatcaagcgc acaccgctgg
    25021 agtatcaacc ctgcagcaag catatctaca gcgatgtcga ttatatgctg cttggattta
    25081 tcgtcgagtc ggttaccggt cagccacttg accgctatgt tgaagagtcg atttatcgcc
    25141 cgctcggcct gacgcacacg gtatttaatc cgctgctgaa aggctttaaa ccacagcaaa
    25201 ttgccgccac ggaactgaac ggcaataccc gcgatggcgt gattcacttc ccgaatatcc
    25261 gcacttccac tctctggggt caggtgcacg atgaaaaagc cttttattcg atgggcggcg
    25321 tttccgggca cgcaggtttg ttttccgata ccggcgatat tgcggtgtta atgcaaacga
    25381 tgctgaacgg cggtggctat ggtgatgtgc agctgttcaa tgcggaaacg gtgaagatgt
    25441 tcaccaccag ctctaaggaa gatgccactt ttggccttgg ctggcgcgtg aatggtaatg
    25501 ccaccatgac gccgacgttt ggcacgctgg caagcccgca aacctacggt cacactggct
    25561 ggaccgggac ggtgaccgtt atcgatccgg tgaatcatat ggcgattgtg atgttaagca
    25621 acaagccaca ttcgccagta gccgatccgc aaaagaatcc caatatgttt gaaagcggtc
    25681 agttgccgat tgcaacttat ggttgggttg ttgatcaggt gtatgcggcg ttaaagcagt
    25741 aataaaaagg ccagtcagtt gtgatactgg ctggccttta atcatcgaat cagaaacgct
    25801 tacaacgcca ttaacttgtc cagcgacggc gcgaagtaat agccgccagt gaccggtttg
    25861 gtaaaacgca acatcgcatc acgtttgcca tcggtatcgc caaacatgct cagcagttgc
    25921 tgctcaatgt tatgcagacg cgcgcagtag gcgcagaagt acagaccgtg cgtaccgctg
    25981 gcggtgccgt acggcaggct ctgtcgaaca atcttcagcc ctttgccatc ttctttcaga
    26041 tcaacgcggg tcaggtgaga ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc
    26101 tctttagtgc gcccgatcat catctcttga tcgtgaatgc tcatccggtt gagttgcttc
    26161 aggttgtgct cccaacgctg cacaaacaca tagctgccgc cggcatccac gccatctttg
    26221 ataaccgcca cttcgcggcg cgtctcttca cccgccgggt tttctgtacc gtcaacaaag
    26281 ccgctcaggt cacgctcttc aacccaacgg aagccgtgga tctcttcttt cacttcaatg
    26341 caatcaccaa acgcttccat cgccgcctgg gcgacagaga agtttacgtc gtgacgcaga
    26401 gaaagaatgt ggatcaacac atcgaactgg gtcgtcggtg caagaccttt accgtagccc
    26461 ggaaaatctt tcagctcttc ggccccaacg ccgccgctca gagcgcgcca ggtattgtta
    26521 ccaaaggcaa ccaccgcacc aagatgcgcg tccgggaatt tcgcttcaaa agttgccagc
    26581 ttgtcggcaa atgttttact ggccgcacgc agggcgtcaa cttccccttt cacgttggct
    26641 tcgatccaaa tcgccgcgcg gcaatgttct ggcaaaatgc cactctgaac ctgagacatt
    26701 gttcctcctg aaaataagaa tgccacgcaa tccgtggcat tgttgggcgc tattttacgt
    26761 gttttttcgc ggcggcggtt tgttcagacg caaattaacg ccgccagata attttactga
    26821 ctttccagtt tttcagggta tcgtccgaag gcattaaccc ttcaggacca ctccattccc
    26881 cgctgaactg gtagctaata tgttgactgc cttcggcttt acattcgacg gcacgattat
    26941 catcaccatc agctttttgg caattgccaa atgctttgct gtaaaggtcg ctaaacggtg
    27001 taccgatttt aacaccggtg tcagcaggaa tatcgctatc cagcacgtca atgcgactga
    27061 tcgtgccttg atcgccgtta atcaccatcg ccacgttgtc gcccttcatc acttcaaaaa
    27121 agcgcaccac gttgccgttc gcggttttca ttccgctgcg caggcgataa tcgccatcaa
    27181 gcgcatcggc aatggcttgt tcttgcagtg gtgtggacgc cgttaattca ccaacgccct
    27241 gctcgctcac tttggtggat gatccaaacc agttccacgg attagcggca gaccagttaa
    27301 cggacgacat cgtggaacag ccggtcagca tcaacggcat agcgcataac attaaacgca
    27361 gcgatttcat gtcacttcct ttggttattc aataacgttg cttggagtgc aaattcacca
    27421 aaaagtgccg ttattctttt tcttcattaa aacaagcgcg taaacgtcga ttggtcagca
    27481 gccagattaa cgccacaata tccgccacta ccagcgccag accgataccg ctaacggatt
    27541 caccgttcag ccacaaccac ggctgccagc aaagtaaaac cacttgcgcc aacagcaaca
    27601 gaaaatagag cacatgccag atacggggaa atgaagcccg ccgaccactc agcaaaaacg
    27661 ccagcaccgc cggaatgccg ggaatcaacc ccagccagaa attatcgtga tcgggataaa
    27721 acagatttag cagtgcagta ccctgctcgc gcgacgcacc ggcaatgaca aacagcaccc
    27781 aggttcgcgc ctgaagcaat agcacaagcc agaagagcaa gggtaaacgc aggcgaccgt
    27841 acgcatcata atggacagga tgaaactcag tactcttcat cttcaatcaa acgcttaccc
    27901 agactcagca cgtcggcgtg ttcatatccc aggcgttcat acattccgag caccatgtcg
    27961 ttatcttccg gcacgttgat ctgaattttc gggcagccgc gagcaatcag ctttttctcc
    28021 aaccgattaa gcaacgcatt agcaatccca cgcccacgaa actctggatg cacgccaaga
    28081 taataagcag acccgcgatg cccgtcataa ccgcccatca ccgttccgac cacttcaccg
    28141 ttcacctcag cgaccagaaa caaactgacg tcatggttca tcttacgctc aatgtccatt
    28201 tccggatcgt tccacggacg cagcaagtcg caacgctccc aaagggtgat gacctcttcg
    28261 aaatcttcct ggcgaaatac gcgtatctcc atggtattcg ttaccttttt gcgggttaaa
    28321 aggctgatta tggcgtgaac ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa
    28381 gtggcataat ggggagttgt caactattga aatgaaaagt aaaacaattc tcaacagcaa
    28441 acggtcgtaa cggattacgc gatacgatat aacatctgga actttattat tacaactcag
    28501 gccgtatgag cacttttaaa ccactaaaaa cactcacttc gcgccgccag gtgctgaaag
    28561 caggacttgc agcgctgacg ttgtcaggaa tgtcgcaagc catcgccaaa gaagaacctt
    28621 taaaaaccag caacggacac agcaagccga aagcaaaaaa atctggtggc aaacgtgtcg
    28681 ttgttctcga tccaggccac ggcgggattg ataccggagc gatcggacgc aacggttcaa
    28741 aagaaaaaca tgtggtgctg gcaattgcca aaaacgtccg ttccattttg cgtaatcatg
    28801 gcattgatgc gcgtttaacg cgttctggcg atacgtttat cccactgtac gatcgcgttg
    28861 aaatcgccca taaacatggc gcagatctgt ttatgtcaat tcatgccgac ggctttacca
    28921 acccgaaagc tgccggtgct tcggtatttg ccctctctaa ccgtggggca agtagcgcaa
    28981 tggcgaaata cctgtctgaa cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga
    29041 ctgacaagga tcacctattg cagcaagtgc tgtttgatct ggtgcaaacc gatactatta
    29101 aaaacagcct gacgctcggc tcgcatattc tgaaaaagat taagccggtg cataaactgc
    29161 acagccgcaa caccgaacaa gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg
    29221 tgctggtgga aacctcgttt atcaccaacc cggaagaaga gcggctgtta ggcacggcgg
    29281 catttcgcca gaaaatcgcc acagcgattg ccgaaggcgt gatcagttat ttccactggt
    29341 ttgacaacca gaaagcacac tcgagaaagc ggtaagtgat gaaacccgac gcacaccagg
    29401 ttaaacagtt tctgctcaac cttcaggata caatttgtca gcagttgagc gccgtcgatg
    29461 gcacagaatt tgtcgaagat agctggcagc gcgaagctgg cggcggcgga cgtagccggg
    29521 tgttgcgtaa tggtggtgtt ttcgaacagg cgggtgtcaa cttttcacat gtccacggtg
    29581 aggcgatgcc tgcttccgcc accgctcatc gcccggaact tgccgggcgc agtttcgagg
    29641 cgatgggcgt ttcactggta gtgcatccgc ataatccgta tgttcccacc agccacgcga
    29701 atgtgcgctt ttttattgcc gaaaaaccgg gtgccgaacc cgtctggtgg tttggcggcg
    29761 gcttcgactt aacccctttc tatggttttg aagacgatgc tattcactgg caccgcaccg
    29821 cccgtgacct gtgcctgcca tttggcgaag acgtttatcc gcgttacaaa aagtggtgcg
    29881 acgactactt ctacctcaaa catcgtaacg aacaacgcgg cattggcggg ctgttctttg
    29941 atgatctgaa cacgccagat ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag
    30001 gctacaccga cgcttattta ccaattgtag agcgacgtaa aacgatggcc tacggcgagc
    30061 gcgagcgcaa tttccagtta tatcgtcgcg gtcgttatgt cgagttcaat ctggtctggg
    30121 atcgtggcac actgtttggc ctgcaaaccg gcgggcgcac cgagtctatc ctgatgtcaa
    30181 tgccgccgct ggtacgctgg gagtatgatt atcagccaga agatggcagc ccggaagcgg
    30241 cgttaagtga gtttattaag gtcagggatt gggtgtaact ccctcccccc cattcccgca
    30301 tccgctgatg cagcgtcagt gacggcttct cggcaaacag ctgctggtaa tccgtggcga
    30361 attgccccag atgccagaat ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg
    30421 accaagggct tatcagttcg cggcgtacgg cgttcaggcg aatgcgtttc agccacgcgt
    30481 ttgggccaat gcctaaaata gcgtgaaacg cgttttgtag cgtgcggcgg ctgacatgca
    30541 gttgattaca caagtccagc accgtcactg gttcggacat gttttccagc acatattcac
    30601 gggcgcggga aagcaatcga cggtaactct gatgactgat gctttccgcc gtcaccattg
    30661 gctgcgcttc ttccagcatc gcccccatcg ccattagcaa attatccccc agcacttttc
    30721 gcactgcagg ctgatggaga ttttccgggt tttcgcaaaa cgtcgccagc gcctgttgga
    30781 caaagcccca cagagcggct ttatgctgct ctttcacttc caacgccgac tggctacgca
    30841 gcatatgtaa tacccgatcc ggattatgca aaaagttagc ctgacgagtg atgacatctt
    30901 cagaaagcac tacgccaagg atcgtgtaat cgtccggcgt acttaattca aactcggttc
    30961 caccggggcg cgtcgctatt tctgcgcttc ccagacattg cgaaccgata aatccctgct
    31021 caccgcgcgt cgccggaatg ccaaaccaga acgagttcgg ccagaccagg cacgactggc
    31081 gcagcgccag accggtgtat tcacggaaaa cctgaatatc atcgagtaga atttccgtaa
    31141 attcaccatg aaacttgccc ggatgcagct gatcgtaaat ctgctgccag gcggtaattg
    31201 tcaaagcatg ttcatagaca tccgttgtct gtcgttgatg aacattatcc acttcgacct
    31261 tcggcgtgag cttcaggttt tcgggtaagg gttcatgata aagatggtgc aaattggctg
    31321 tacgggtctt tttcatgatg ttaatgccgg gtgttgtagg acacccggca cctccgacag
    31381 gttaatgggg cttgagacga taacgactac tgcgtttacg taacgtcccg gcagaaaaga
    31441 gctgttcgag cgcatttctg gctttttcca gcggccagcc aaagtgggca gccacttctc
    31501 ctgccgtcat tccctgacgt actgatgtta acagcgccaa caactcgtca gcagattccg
    31561 cgacgataac tggcgcgtcg ggttccttta cgggttgctt aggctggcgg ttaaagccag
    31621 taaccagcca ctgggtgtca tcgtctggcc gaccgatctc cttacggctg atgacacgcc
    31681 cggtacgcat cgcggcagca caacctgcgt ccagcgccgc acgacacgcc gccagatcgc
    31741 cttccaccac cagcgttagg cgaccagggt caagcacttc gtgactgagc agacgaacgt
    31801 tagctgcttt gagcatggca tcagctgcat ctatcgcggc gaccatgccg tccacttcca
    31861 gcaatcccag tgcattgatc atcggcaacc tccgttacgc acgctggatt ggattacgcg
    31921 cgatttccag cactgcatcg gtaaaggcgt tacaggctgc tttacacgcg gcctggctac
    31981 cggttaaaaa tgctgccgag tagttggttt cagacggtgg cgggacatag gtcaccagct
    32041 gtacgtcggc agatttcagg gctgcatcaa tgccgtaggt cgcttccagc ggcggtgcca
    32101 ccagatacgc catcggatcg ccaagcgtga tcccggcggt tgatgagaga taagaaccgg
    32161 tacgcgaaac tacatgtgcc aggaaggctg tgttttctgc gtcattagcc cactggaaag
    32221 cagcgccatt ttcaatatgc gcaaccatcg catccagacc agcacgcact tccgccgggt
    32281 ttggcccacc aagcataatc agcacttcac cggcagtcgg tgacgggccg tgtgcggctc
    32341 ccgcatacag cgagcggcca tacaccactt ccaccatcgc ctgcttggtc gcttcgtcag
    32401 ccgcaatgta ggtgacgtca tctgaatcag cagaaatcag cccgaggcta cgaatatgcg
    32461 gcggcaattt cagttcacgc gcaaattcgg cgttaacgga ggcaatcacc cgcatggcgg
    32521 ttaccgacgg tcgaatcaaa tctaaagctg gcatgatgcc tccttatcgg gtcatgttga
    32581 tgccggatgc tttctgctcc agcatacgtt tcgccaaatc cacaatgacg gctgcggctt
    32641 caaccggcgg cgtgccgccc tggtgaatgt tagagataca ggtacggtcg gcctcaacgg
    32701 tggttgccat acgcggcgag taaacggcgt agcaggagag gctttctgac tgccctaatc
    32761 ccgggcgttc ccccaccagt aggatcacta ctttcgcgcc gaggatctca ccgatctgat
    32821 cttcaatctt cacgcgacca taacgaacaa agaatggtgt accgactttc agcccggcct
    32881 gtttcaggcc cgccatcagc ggtggcagga tctcttcgta gttcacggtg atcgcatcag
    32941 ttgacaggcc atcagaaata acgacctgta cgtccggatt agcaacacac tgtgctttca
    33001 gcgcttcaac agcttctgca cacaggcggc ggcccatatc cggacgagtc aggtagagat
    33061 ttttgtcgct aatttcagag cggacttcca gcaagccctg cgctttcacc cactcttccg
    33121 gtacttcttt cagtacggta tctttcgaac gagagtgatc ggccaggaaa cgcagcaacg
    33181 cctgggtacg cggacgcgga ccggcacgac cggtacaaac gcgggccacg gtgctgcgac
    33241 gcagttctgt taatacgtct gcgcgatgcg ggttttccac accaatccac gcttttgctt
    33301 ccgcagaacc taaatccagc gcgcagcttt ccgaggtcac tggtgtcgta caggtggtgg
    33361 tggcgcactt tgcttctgac ggcgcggggg ccgcttgtcc cattgacgcc atcacgctgc
    33421 gtacaatttc ttcaatctgt ttttgatcca tgatgtgtta tctccgcgtc atcagaagaa
    33481 cagtgacgga tcgcccgccc gtttggtcag gcgaccgttt gccataatgc ccatgctttc
    33541 cagccagcgt tcaaactccg gtgacgggcg caggttgagt aactgacgca cagtggcggt
    33601 gtcgtggaag gccgtagtct ggtagttgag catgatgtca tcacccagcg gcatccccat
    33661 gatgtagtta cagcctgcgg tggcgagcag gatcatcagg ttttcgttga ggttctggtc
    33721 agcgtcagcg tggttggtgt agcagcagtc acagcccata gagatgccgc tcagcttgcc
    33781 cataaagtga tcttctaagc ccgcgcggat aatctggcgg tcgttgtaga gatactccgg
    33841 cccaataaaa ccgaccacgg tgttgacgat aaacggatcg taatgacgcg ccagcccata
    33901 gttacgcgct tccatcgtca cctggtcagc gccgaagtta gcgccagcgg acagcgccga
    33961 accttgtccg gtttcgaagt agaggcagtt ttccccggcg atacggttaa actctgcacc
    34021 cactgcgcgc gcttcgtcga gcatcgccag ctccacgcca aactctttca gccctttttc
    34081 gctgccgcaa atactctgga aaatcagccc gcccggtgcg ccgcgacgaa tcgcttcgat
    34141 ctgggtggtg acgtgcgcca gtacgcagcc ctgagttggg atgttgaatt tgtcgatcac
    34201 gccatagatg gtatccaaca cgcggcttaa gttttccacg tcgtcagtca ccgggttaac
    34261 gccgatcacc gcatcgccca ccccgaagga aagcccttcg tagatttgcg cggcgatact
    34321 ttgcacatcg tcacgggtat cgtttggctg caagcgggcg ctaaaggtgc ccggaatacc
    34381 gatggtggta ttggcctttt tgatcaccgg cattttcttc gcgccgtaga tcaggtccgc
    34441 gttggagcaa atcttcgcta ccgccgctac cacttccgag gtcagccctt tgcgggtaaa
    34501 ggcaatgtcg tccacgctgg tttcatcgct cagcacatac tcacgcagtt cgctgatgct
    34561 ccagttttta atctggttgt aggccgtttc gttaacatcg tcctgaatca gccgcgtcac
    34621 gcagtcatct tcataggcaa tcaccggatt attgcggatg tccgctacgg tcatttccga
    34681 caacacctgc tttgccgcca cgcgctcctg tgagcttgcc gcagcaacgc ccgccagcac
    34741 atcccccgaa cgcagttcgt tggctttagc cagcacctct tttacatcct taaactgata
    34801 tacattgccg aacaatgtgg tctttagttt cataagtcgt tccctcagga aggaaatgcg
    34861 agtgatttca ccgtcaccgg cacaaccgat ccgccaaaaa gaggcgtacc aatgtcgata
    34921 tagtcccccg cgcggacaat cacttcgtca atgactgcca acgggagttg ttgtagctgt
    34981 gggcgcaaca acatgcccag agctttacca aagtcctgcc cggccaccac cagcagggga
    35041 tgcggattcg gaaaacgcgc gacgaaatcg accaacgcgt tgatgaccgt cagtaccgcg
    35101 gcgtaacgca caggcagcga ggcgggaagc gccagcacgt acgcgtcagt tttaggatca
    35161 agatccagct gaatcagcgc ctgttgccag gcactcacca gatccttttc atcaattggg
    35221 atcgccaccg gcaagttgcg cagcggcagt tgtacgccct ccagccagat tgtgctgcca
    35281 gagagcgaaa gggtatgtgc acccgcgcca atcaccgtgg cgcgcacggt ttgcgccgga
    35341 aactgcacgt tcatctcacg caggcgcgga tggtcatgca gcgccgttgc cagaagcggg
    35401 ccaatatcgg caaaacagaa cgggtcggcg ggctggtggc gataacattc gccaacgccg
    35461 ccagaaagcg taatgatttc gggcgtaaca cctacgggca gcaaaccggt ttgcatcaat
    35521 gcctgcgcga gcggcgaaag cgttccgtca atcacttcga caatcagctc tgccatccgc
    35581 cgggtaacct gcaccagctg cgcgccggtc agcgaacggg cttcggtgcc tgcaccgaag
    35641 cactcatcca caatcatctg ccccggttta tgagcgtaaa ccacacgccc ctggctgtcg
    35701 gtttccagca ggcgaccgcc gacattgagg caggcggtgc cgctgatttt tccggcatcg
    35761 aacagggcgt agttcgcggt gccaccgccg atatcgatat tcaggacccg acacagccgt
    35821 tgttcagaaa gggtttgcgc cccggcaccg tgaccggcga tcacggattc gagatgcggt
    35881 ccagcgctgg cgacgacaaa atcgcccagc gactgggaga gcgtcatcac cgccgggcga
    35941 gcgttgcggg ttttcgcgct ttcaccggtg atgatgatgg caccagaatc aacgctttcc
    36001 ggcgcaatgc ccgcagcctg atattgctcg aggattaagg ttttcagttc cgcttctttt
    36061 aaaccaccct gtttatcgac aggggtaaag aacaccgggc tttgccagct aatttcgcgt
    36121 ttgatgaatt cgtagcgcgg cacctgcgac accgccgcac ggttaaccag ctccaggtgg
    36181 gagaaaatca cctgggtggt ggtggtgccg atatcgatac cgacgctcag tagctggcga
    36241 gtgttcacga ttgcgcctcc gcttcggttt tagccgcgct cgcgtcttct ttaggtacca
    36301 gcatcatcgc cacgccaatc gccgtgacgc cgccgatcag cttgccgaca atcatcggga
    36361 agatcatggc gttcatgttg gcagccgcga agcctaaatg gtcgcccagg gcgaaagcag
    36421 cggaaacggc gaaggcacag ttgatgactt tgccgcgggt atccatctgc ttcatcatgc
    36481 caaacatcgg gatgttgttg gcaagcgttg ccaccatgcc ggctgccgct atattgttca
    36541 tattcagcac cttaccgaca ctcatcagcg gtttttcaaa ccagcgagtc agcagcagca
    36601 ccatcggata cgcccccagc agcacgcagg agatagaacc gataacttca atggcgcgca
    36661 tcacttcacc gggtttgtcg cctggagcca tgaagatcgg atccagaccg gggatcagtt
    36721 cccaaccgag caggaatttc actaccgcag cggcaagacc gagggtgatc aatgcaacga
    36781 ggaatttggc gaagatctgg aagccgttga tcattttttc cgggatgaat ttcagcccca
    36841 gcgccaccag caccgcaacg ataagtaccg ggatcatgtt catcaggatc agcgcaaagg
    36901 tgaattccac cggctgaccg ttgatctgca caccggagta catagcaacc agaccaccag
    36961 caatacagcc aatcggaatg gtcacaatgc ccgccagcac gccgagcgcc agataacgac
    37021 ggtcagaagg ttcgataatg ccgagcgcca ccggaatgga aaacacaatc gttggcccca
    37081 tcatcgaccc gagaattaac ccagagtata gccacgctgc tacgtcgccg cccgccagct
    37141 ctttggcgag gaagaagccg cccatatcgc acgccagcag tgttccggcg aacatcgatg
    37201 ggttagcgcc gagcatttcg taaaccggga taatcaccgg cccgagtacg tgggccagta
    37261 ccggtgccag cgcggtcata ccgaccatag ccaggcccaa agcgcccatt gccataaagc
    37321 cttcttcgaa ctgaccgccg gatccttcga tacttttacc gaacttaccg aggaaacgag
    37381 cagaaccgcc gaactgcgac aggatcctgt ctacggcagc tatcagcata aagaacatca
    37441 tgatgtacat gatgatttcg ttaataccca tcgcctttac tccctgttag ttgttattta
    37501 ttggcggatg cggcgtgaac gccttatccg ccctacatgt gcaatcccga aggccagata
    37561 agacgcggca agcgtcgcat ccggcaattg caccgcgcca ctggcggatg cggcgtgaac
    37621 gccttatccg ccctacatgt gcaatcccgt aggccggata agacgcggca agcgtcgcat
    37681 ccggcattta ttgcgccgct gcgtacaggc cgacaatctg ctccaggctg gcggtacgcg
    37741 ggttactgcg cagacaaata tcttccagcg cggcctgcgc ccatgcgccg taatgcgcag
    37801 atgtcgcacc aacatcgccc agtcgtttac caatcccaac ttccgctatc agctcactta
    37861 ccgcattaat agcgtcacga tcgtcggatt ttttagttcg cagcgcccga ccaaccaagc
    37921 taaaccgatc acgacaaacc atccggttaa attccatcac cgttggcaac aacatggcgt
    37981 tcgccagacc gtgcggaata tgcagcgccg cccctggctg atgcgccatc gcgtggcaca
    38041 acccaagtcc ggcactggaa aacgccattc ccgccataca tgaagccagc aacatgctct
    38101 cgcgcgcggc aaggtcatga ccgtagccca ccgctttcgg cagcgatttg ccaatcatcg
    38161 caatggcacc aatcgccaga ctgtcggtaa acggtgtggc gttcagcgcg ctgtacgctt
    38221 caatggcatg ggttaacgca tcaatgccag tcatcgccgt gacatgcgac ggcacacctt
    38281 cggtcaatgc ggcgtcgaga atcgccacat ccggcatcag cgaggcatgg gctaacacct
    38341 gcttgcgccc gctcaccgcg tcgataatca ccgttacgtt ggtggtttca gagccggttc
    38401 cggcggtcgt tggaatggca atcaacggca ggcgcggttg cagaacgctg gtttctgaca
    38461 tttctgccag cgtgctatcg gggttcgtca ccagcaacgc cacggctttc gccgcatcca
    38521 gcaccgagcc gccgccaaac gcgatcaccc cgtcacaacc tgattcacgc aactgcgcca
    38581 cggctgcaca cacgtcggtg atgcacggtt cgcccaccgg acatggccag agcgtcatgg
    38641 cgataccttt aacggccagg ctacgcgtca gcccggcggt catccccgcc tgatgcaaaa
    38701 agctgtctgc catcacgaac agatgtttca gcccacgcgt ttgcgcttgc tgcccacaac
    38761 tgctcaccgc gcccggaccg caaagcgtca ccggtggaac gctaaatgtt ttcacccgct
    38821 gcagattcag ggtatcgaat gcctgaaaaa gcgccgtttg caattcactt tgcatagatc
    38881 ccctccgttt tctctcgccc actactggcg atcgccagcg gggtcataaa caaactgtgc
    38941 tgcggtaaat gcacctgtaa cgccgggaat tgtttgcgaa acagttccgc tacgcccggt
    39001 tgcatacagg aaccgcccgc cagccataaa tccgtaatgc cctgcccttc aatatgccgg
    39061 gccacaatgt ccgccatttt ttcgtacacc ggtttcaccg cgggccaaat ctcgtcaccg
    39121 tgaccgcgtt tgtactgctc tgcctcttcc agcgaaatac ggcgatttcc ggcgagagtc
    39181 agagagatgt gatgtccgcc ggtggcttca tccgccgagt acgtcacttt gccctttttc
    39241 acgatggcaa tgccggtagt gccgccgccg atatccacta caccggcgtt gtccagttgc
    39301 agcagatccg caactgcggt tggctcatcc agcacatggc tcacttccag tccagcagac
    39361 tccagcacgt tgatggaaat acgcgggtcc gtacctggag gaaaagaggt cgccgcatgg
    39421 ctaaaacggc gaccgaattg ctgctcgagg gtgtcgagat ggcgacgaac aatggtgacc
    39481 gcaccgaaga aatcccagac gatgccgtcg cggaccacat cagcccagtc gaggcatacc
    39541 gccaccggct gaccgtcgcg gtcgacaacc atcgacacca catcgcaggt gcccagatcc
    39601 acccccagcc acagcggaga ttccgtcgcg gcgggcgtct ggttacacag cgttgccgcc
    39661 gtttgcagac gtggggtgag ccattgttcg tcgtgcgcca tctgttactc cttaaacaat
    39721 gcgaaacgca tcgactaata cacagcgacg cagacggaca aacgtccgcg cgctggttac
    39781 cccttcaccg gttggcgtgg tgatggtcat agtggtccag ccttccccgc ccagccccag
    39841 cccggcaatg cacggtccgt tcttaacgaa aatgctggta tcaatggcat tcgccatctg
    39901 gttcatgttc tcgatattgc gcgagtgcat tgccgccgtg tggtggcaac cgccttccag
    39961 tttcaccgcc agcgcaatgg catccgccac gttggcgacg cgcacgacgg gcagcaccgg
    40021 catcatcagt tcagtcacag caaacggatg ttctgcggtg gtttctacaa acagcagtcg
    40081 cgtttgttcc ggaactttaa ggccgattgc cgccgcgatt ttcgccgcat cgcgaccaac
    40141 ccagtcacgg ctgacggtgc cttttccgcg ctcgtcgata tttttcagca acaccgattg
    40201 cagttgctgc gcctgctctg cggtcagttt caccgcatgc tggccttcca tcagacgcat
    40261 cagttcatcg gctacgctat caacaacaat cagtaccttt tcgtcggcac aaatgatgtt
    40321 gttatcgaaa gaagcacctt tgacgatgga ctgagctgca cgggcgaggt cggcggtttc
    40381 atccaccact accggcgggt taccggcacc tgccgcaatc aggcgtttat tggtgtgttt
    40441 gcgcgccgct tccactaccg cttcgccgcc ggtcaccacc agcaggccga tacccggaaa
    40501 cttgaacaag cgttgcgcgg tttcgatatc cggatttgct acggtaacca gtaagttttc
    40561 cggcccgcct gcggcaacaa tcgcctggtt gagcagcgta atcgcccgct gggagacttt
    40621 tttcgccgcc ggatgcgggg caaaaatgac gctgttgccc gcggcaatca ggctgatggc
    40681 gttattaatg acggttgccg ccgggttagt ggaaggcgtc accgaagcca ctacgcccca
    40741 gggcgcgttt tcaattaaag tcaggccgtt gtcgccagtc agcacctgtg gagagaggca
    40801 ctcaacgcct ggtgtgccgc gcgcctgagc gacgtttttg gcaaatttat cttcaacgcg
    40861 ccccatgccg gtttcactga cggcaagttc cgctaaatct ctggcgtgtt tttcgcctgc
    40921 ttcacgaatg gcagcaatgg ctaactggcg cattgccacg ctttttaacc cttgctgggc
    40981 gactttggct gccgcaacgg cgtcatccag ggacgcgaaa acgcccatct catgaacggc
    41041 ggcgggcggc gtgtcactgc tttgcatttt cagcagtacc gctttcacca cctgttcaat
    41101 atcctgttga ttcatgatgt tctgccttat ttgtggaaaa tgacctgacc gccagacacc
    41161 acctcatcga cgatgccaat cacgcacaga tcgaccggtg acgtttcgct tttatgcgcc
    41221 tggcgggcgg aactgccact caccagcaac acccactccc cggttcccgc gccaatattg
    41281 tcgatggcga cggcgcattg cccgtcagga ttaccttgtg gatcaatcat ttccaccatc
    41341 agcaatttgt catgcgccag tccgtgatgg cgtacggtac aaacaatttg tccagtgacg
    41401 actgccagtt tcatacccgc ctccgtggcg tatttcaggt aaaagctccc cctaccctcc
    41461 gcagaaggta aaatgaaaaa ggagagagcg tgacgcccga atcgacgtca cacagggtga
    41521 ttacaggttg ctgctatcgc ctttcaggcc gatcgggaag acttcttcca ggtcgccgtg
    41581 cggacgcggg ataacgtgta cagataccag ctcgccgata cgctgtgccg ctgcggcacc
    41641 tgcatcggtc gcggctttac aggctgcaac atcaccacga accatggcag tacacaggcc
    41701 gccgccaatc tgcttaacac caaccagctt gacgcgcgca gctttaacca tcgcgtcaga
    41761 agcctcaatc agtgcaacca ggccccgggt ttcgatcatt cctaatgctt ccattgtgct
    41821 ttcctcttta tcagggtcca gaacgggacc gttcattcaa ccagtgtttg taaactgctt
    41881 tcgcggttca cttctgtctg acgctgcaca gctgccacca gcgccagttc gataatttcc
    41941 tgcacgctac aaccacgaga gagatcgtgc atcggcgcgg caagtccttg tatcaatggc
    42001 ccgacggcac gatatccgcc aagtcgttgt gcgattttgt agccaatatt tccggcttcc
    42061 agcgacggaa aaaccatcac attagctttg ccctgtaacg ggctggcagg cgctttttgc
    42121 gccgccactt ccggcacgaa ggcggcgtca aactgcaact cgccatcgac aaccagcttt
    42181 ggtgcgcgct cacggacgat ttctgtcgcc tgctggacgt tggcaacgca ggggtgacgg
    42241 gcactaccgt tgctggaaaa cgacaacatc gccacgcgcg gttcttctcc ggtgatggcg
    42301 cgccaggttt cggcgctggc aagcgcgata tccgccagtt gcgccgccgt cggctgtggc
    42361 actacgctgc aatcggcaaa gcccaatgcg gggccactgt actgtggcag catcaggaaa
    42421 atggaggaga gcgttttaca gcctggctgt aagccgataa tgcgtaatcc ggcacgcagc
    42481 acattcgccg tggaagagag attgcccgcg atacagacat ccgctgtccc ggcgctgacc
    42541 attgcggcag cgaacatcag cggatcggtg agtttatcca gcgcatccgg cggcgttttt
    42601 tcgcccgcac gggccagcca gcggagagca aattcttccc gcattgcgag gttgccatgc
    42661 ggatcaatca cctgtagccc gtccatcgcc acgccgtgac tgagcgcaaa ctgacgaagt
    42721 tcaaacggat tggcgaccag aatgggcgtt gccagacctt gttgatgtaa atattgcgcc
    42781 gctttcagca cacgttgatc taacgcatcc ggaaaaacta ctctggcggg cgctcgcaac
    42841 gccagtttac gacaacgttc aataatcatt gcgtttcccc cagtcgttgt tggatctgct
    42901 tcaccgttag cgactgcttc gttacgctca ggatcatcat cacgtaaacc gtgctggaaa
    42961 ggcggttcag cgcctgcaag atgtccgggc gtaatacttc aaagctgcgg gtgataaaca
    43021 cctgcgccgc tacagtttcg gtttcccgca ctttggtgcg tagcagattg agcaacgccg
    43081 catcacgccc gtggctggct tccggcacca gatgatcgtg gtcgagatag cgcagcggct
    43141 ggtgagaaag ccgatgcaga tcttcgtcag aaagcccgac aatcgcttgt tcgcccagcg
    43201 gttcccccag cgcatcggcg cgcataatgt tgccaagacg cgaacggata tccgccagcc
    43261 acggctgcca cggttccgcc agttcaattt gcagccacac tgccagcgca atggtgctgt
    43321 cgagaacagc gcgaaaagcc aggcgcggat cgcttttggc aaccattttt tccgccgaca
    43381 ggtgagtcag cgtatccggc ttttttgcca ccggctggcg gcacagttcg cagcacgcct
    43441 gcggatgttc atcgctactg gttagcccgt gaacgggctg cggctgctgc tgttcatcgt
    43501 caacaaacag gcggccctgc tcgtcaataa acttgatgcg cagatggcgg ctttccagta
    43561 actcccgggc agagggcgtc aggcgactgt ccgcagggag atggatctct gcgccttcgc
    43621 tgagcgtatg attcgctctt agccatgctt cggtgatgaa atctttcata aggattgcca
    43681 gttagccggc caggcgacat acaggaattt cacgctggat gtcgtaccaa attcgatgct
    43741 ggagcctttc gggataaaca tcacatcgcc cgctttggcg atcatagttt cgccttcgtg
    43801 gcgaacatgc agctcccctt ccagcaccat gtcgatttcg tcgtagttca gggtccacgg
    43861 aaagaaggcg ttctcccact gcatgaaacc agcggccata ctgctgccgt cgtctccggt
    43921 cacgagatca gtcaagccta cgcagtgcgg ttccgcgcca tcgaaacgac caaacttgac
    43981 gctgctgcca tcaataactt tgatgccgcc tttgccggtc accgatttaa agctcggctg
    44041 cagtgcaccc tgctccagcg actgtttttc cttcatcact ttttccatca gctgcgcgac
    44101 caggctttcg gtaaactgac cttccggcag ttgggcgata atggtttcac ggatgcgctg
    44161 gctttcggtt ttatcggctg atgcgctggc gggaacagac gccgttaccg ggatcgattc
    44221 atcacattcg gtgatggtaa aacccaacag atcagccact tcacgagctt ccggggtaat
    44281 aatgctggcg cgcagaacca cggacattgc cagttcgccg cgcgcgtgtg cttcacgaat
    44341 atcattcgct gtgatgagtt ttttcacctg cttcctcctg tttggtgaga gatgccagat
    44401 aatccaccag ttgctgcacg ctttgcgggt cgtggctgtt tagctcgaaa atcggctctt
    44461 cgaagcccgt ctcaagcaat aactttcgcg ttgcggcgac gtcagcatct ggcatgtccg
    44521 ttttactgat gacggcgatt tgccgtttgc tgacgccaat atccagcaac ccggcaggca
    44581 accgactttc cgggtcattc gcgccgtgga catagatcag catgtccaca tcctgtagcg
    44641 tggtaattaa tgcgtgatac cagcggggat ggctaaaata ttcacccggc gtatcaatat
    44701 cgccattatc attaaattcc acggcctgcg tttttctggc gagggtataa ttcccctgca
    44761 acgcattaaa taacgttgtt tttcctgccc cgacggtacc cacaaacgca attcgtttca
    44821 tgaatgcctc atattaactt ttggtcattt cacaaagcgt ataatttaat aagcgtccta
    44881 aaccactgac ggtttgtgat aacgcttctt ctactgcgcc caccgaacca taaatcacca
    44941 gcgcgccgct aaaacggtcg agaaaaccga tatgcacatc ggcggctttc aacgctaaat
    45001 caccggcgat catcgccgtt tctccgggag ttagcgtcat aatgccaatt gcgcccgcat
    45061 ccggaacgcc gattttcttc gccagttcct cgccgggatg cgcgatgaga tgcgccagcg
    45121 tgacctgttt gccgggcaca aattcctgaa tgatgcgttc tttatccatg agtcgccgcc
    45181 tgtgaaaaac tttcgtgtat cgtgacaatt tttcgtcaga gtaaataaca aaattcggca
    45241 agcgcgttta aaaggtgagg tagatcacta agaatggaga gatatttact gttatgaata
    45301 aactttcata tgcgacgcat gaatataaat aaaataatat cgagatattt tatatactga
    45361 tttttttatt attcgtaaac tcagtaataa gctttactaa tatattgcag ctaaactgct
    45421 taccctgaat attcagggta agcgtgagag ttaaaaaaaa attacagcgg ttgggtttgc
    45481 gcttcaacca cggccagcgc caccatattg acgatacgac gtaccgatgc gatcggcgtt
    45541 aacacgtgaa ccggtttcgc cacacccatc agcaccggac cgacagtcac gccttccgag
    45601 ctggaaacgc gcagtaagtt gtaactaatg cgggcagctt ccatgttcgg catcaccaga
    45661 atattagcgg aacctttcaa agggctgtcc ggcatacggt cattccgaat cgcttccacc
    45721 agcgctgcat cgccgtgcat ttcaccatca atcatcagtt ctggtgcacg ttccctgacc
    45781 agttccagcg cctgacgcat tttgctcgac gacgggcagt cagaagaacc aaagttggag
    45841 tgcgacaaca gagccacacg cggctcaata ccaaaacgac gaacagtttc tgccgccatc
    45901 aaggtgatct ccgccagctc ttctgcatca ggctcatcat tgacgtaggt atcggcaata
    45961 aaggtgttac cactcggcaa cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg
    46021 cgataaccaa agacattttt caccacgcta aagtgttcat gataatcgcc caccgtaccg
    46081 caaatcattg catcggcttc gccacgctga accatgatcg cgccgatcac tgtcgggtta
    46141 ctgatcagcg cacgctgcgc ctgctcctga gtgacaccgc gacgcttcat gatctggaag
    46201 tattcggtcc agtactcttt aaagcgcgga tcggattcgt tattgacgat ctcaaaatca
    46261 acgcccgcct tgatctgcaa gcccagtttc tgaatgcgca tttcgatcac gttcggacga
    46321 ccaataagga tcggtttcgc cagtcccagc gttaccagtt cctgagtggc atgcagaacg
    46381 cgcgcctctt ccccttccgg cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa
    46441 atcggcttca taaacaggtt agttttgtaa acgaactccg tcagcttgtc gatgtagacg
    46501 tcgaaatcag caatcggacg cgtcgccacg cccgactcca tcgccgcttt cgccaccgca
    46561 ggagcgatct taacgatcaa gcgcggatca aacggttttg gaatgatgta ttccggacca
    46621 aagctcagat cctgatcgcc atacgctgaa gccaccactt cactctgttc cgcatgggcc
    46681 agttctgcaa tcgcgcgtac cgccgccagt ttcatctctt cgttgatggc ggttgcgcca
    46741 acgtccagcg cgccacggaa gataaacggg aagcaaagga cgttgttcac ctggttcggg
    46801 tagtcggaac ggccggtaca gatgatggca tccggacgca cttctttcgc cagcggcggc
    46861 agaatttccg gttccgggtt cgccagcgcc aggatcattg gcgcacgggc cattttcttc
    46921 accatttcct gggtcagcac tttcgggccg gaacagccga ggaaaatatc cgcgccttca
    46981 atcacatcat cgagggtacg tttaccgtca tccaccaccg cgtatgcggc tttggtttcc
    47041 gccatgtttg gctcacggcc ccgatagata acgccttttg aatcgcaaac cacgatgtta
    47101 tgtttttgca gacccagcgc taccagcagg ttcatacagg cgattgctgc ggcacccgcg
    47161 ccagaaacca ccatccgcac gtcggagatg tttttctcca ccacgcgcaa accgttgagg
    47221 atggcggcag tgctgataat tgccgtgccg tgctgatcgt cgtggaatac cggaatattc
    47281 atccgctcgc gcagtttctg ttcaatatag aaacattctg gcgctttaat gtcttcgagg
    47341 ttgatgccgc caaaggtcgg ttcgagcgca gcgacgactt caataaattt gtccgggtcg
    47401 agttcatcaa cttctatgtc aaatacatca atcccggcga atttcttaaa cagaacgccc
    47461 ttgccttcca tcaccggttt gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc
    47521 gtaccgttag agatcaccgc taccaggtta ccgcgcgcgg tatatttgta ggcttttaac
    47581 gggtcttttt cgatttcaag acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga
    47641 tcgcgctgcg ttgccagagg cttggttgga gaaacctgga ttttccctgg aactggaaat
    47701 tcatggaaat caagtgcact ttgttttaac tggtcatcca tttggttgtt cctttcacgt
    47761 aacgttcaca aataaagtgt gtgggcaaca gcccctgccc acaacgtggc gcacattatt
    47821 accttgccgg agtctacaga ctttgagcaa gtccaaactc tcaccattaa tataatgttt
    47881 tggtaataat cctataacac tgatgttacc tgcttaatcc agcaatacca tgcctgtctg
    47941 ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac gcactcatct
    48001 aacactttac ttttcaagga gtatttccta tgaacgagtt agacggcatc aaacagttca
    48061 ccactgtcgt ggcagacagc ggcgatattg agtccattcg ccattatcat ccccaggatg
    48121 ccaccaccaa tccttcgctg ttactcaagg ctgccggatt atcacaatat gagcatttaa
    48181 tagacgatgc tatcgcctgg ggtaaaaaaa atggcaagac ccaggagcaa caggtggtcg
    48241 cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtc
    48301 gcgtgtcaac agaagttgat gcacgcctct cttttgataa agaaaagagt attgagaagg
    48361 cgcgccatct ggtggacttg tatcagcaac aaggcgttga gaaatcacgc attctgatca
    48421 agctggcttc gacctgggaa ggaattcgcg cggcagaaga gctggaaaaa gaaggtatta
    48481 actgcaacct gacgctgctg ttttcttttg cacaggcgcg ggcctgtgca gaagcaggcg
    48541 tttttctgat ttcgccgttt gtcgggcgta tttatgactg gtatcaggca cgcaagccga
    48601 tggacccgta tgtggtggaa gaagatccag gcgttaaatc agtacgcaat atctacgact
    48661 actataagca acaccactat gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac
    48721 aaatcctcgc cttaaccggc tgcgaccgac tgactatcgc accgaatttg ctgaaggagc
    48781 tacaggaaaa agtttcgcca gtggtacgta aattaatccc accttctcag acgttcccac
    48841 gcccagctcc catgagcgaa gcggaattcc gttgggagca caatcaggat gcgatggcgg
    48901 tagaaaaact gtctgaaggc attcgtctgt tcgccgttga tcaacgcaaa ctggaagatc
    48961 ttcttgccgc caaactataa accagccacg gagtgttata tgtcccgaaa agaccttgcc
    49021 aatgcgattc gcgcactcag tatggatgcg gtacaaaaag ccaattctgg tcatcccggc
    49081 gcgccgatgg gcatggctga tattgccgaa gtgctgtgga acgattttct taaacataat
    49141 cctaccgacc caacctggta tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg
    49201 atgctgctct acagtttgct gcatctgacc ggttacgacc tgccactgga agaactgaag
    49261 aacttccgtc agttgcattc gaaaacccct ggtcacccgg aaatcggcta tacgccaggt
    49321 gttgaaacca ccaccggtcc tcttggacaa ggtttggcga acgccgtcgg gctggcgata
    49381 gcagagcgta cgctggcggc gcagtttaat cagccggatc atgaaattgt cgatcacttc
    49441 acctatgtgt ttatgggcga cggctgcctg atggaaggta tctctcacga agtctgttcg
    49501 ctggcgggca cgctggggct gggcaagctg attggttttt acgatcacaa cggtatttcc
    49561 atcgacggtg aaaccgaagg ctggtttacc gacgatacgg caaaacgttt tgaagcctat
    49621 cactggcatg tgatccacga gatcgacggt cacgatccgc aggcggtgaa ggaagcgatc
    49681 cttgaagcgc aaagcgtgaa agataagccg tcgctgatta tctgccgtac ggtgattggc
    49741 tttggatcgc cgaataaagc aggtaaagaa gaggcgcacg gcgcaccgct gggtgaagaa
    49801 gaagtggcat tggcacggca aaaactgggc tggcaccatc cgccatttga gatccctaaa
    49861 gagatttatc acgcctggga tgcccgcgaa aaaggcgaaa aagcgcagca gagctggaat
    49921 gagaagtatg cagcctacaa aaaggcccat ccgcaactgg cagaagagtt tacccgtcgg
    49981 atgagcggtg gtttaccgaa ggactgggag aaaacgactc agaaatatat caatgaatta
    50041 caggcaaatc cggcgaaaat cgctacccgt aaggcttcgc aaaatacgct taacgcttac
    50101 gggccaatgc ttcctgaatt gctcggcggt tcggcggatc tggctcccag caacctcacc
    50161 atctggaaag gttctgtgtc gctgaaggag gatccggcgg gcaactacat tcactacggg
    50221 gtgcgggaat ttggcatgac cgctatcgcc aatggcattg cgcaccacgg cggcttcgtg
    50281 ccgtataccg cgacgttcct gatgtttgtt gaatacgctc gtaacgccgc gcggatggcg
    50341 gcactgatga aagcgcggca gattatggtt tatacccacg actcaattgg cttaggggaa
    50401 gatggtccga cccaccaggc cgttgagcaa ctggccagtc tgcgcttaac gccaaatttc
    50461 agcacctggc gaccgtgcga tcaggtggaa gcggcggttg gctggaagct ggcggttgag
    50521 cgccacaacg gaccgacggc actgatcctc tccaggcaga atctggccca ggtggaacgt
    50581 acgccggatc aggttaaaga gattgctcgt ggcggttatg tactgaaaga cagcggcggt
    50641 aagccagata ttattttgat tgccaccggt tcagagatgg aaatcaccct gcaagcggca
    50701 gagaaattag caggagaagg tcgcaatgta cgcgtagttt cgctgccctc gaccgatatt
    50761 ttcgacgccc aggatgagga atatcgggag tcggtgttgc cttctaacgt tgcggctcgc
    50821 gtggcggtgg aagcaggcat cgccgattac tggtacaaat atgttggtct gaaaggggca
    50881 attgtcggga tgaccggcta tggggaatct gctccggcag ataagctgtt cccgttcttt
    50941 ggctttaccg ccgagaatat tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc
    51001 tgatggtgat cgccggatgc tatatgttcc cctcacccta accctctccc cagaggggcg
    51061 aggggacggt ccgtgcgctt tttaggggcg catctgggat tttgtttaac gggtgatcca
    51121 caacgtgggc caggcgtctg gcccatgcca gttatcgcag gtgggttctg cggcgtaacg
    51181 caccaggcga aaacgctgac cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa
    51241 tccgcgccct ttcgctaagg tcaccagttc acgcgatttt tcatcgaatg ttgcgttcat
    51301 cagttccagt tcattcgtct cctgaccgct gttgaacggc aaacgtaacc gaaccgggcg
    51361 cgaggccagt ggctttttac gcgacacaat ccatgccaaa tcaatggtgt tataggcccc
    51421 tgcctcacag ctaatcatca tcagcgcttt atcatcggtc agcgcagtga cattcacctc
    51481 tcgacgcaat ggatcaagcg agcagcgcag accattcatc cgccagtttc cataatccag
    51541 caaatcgttg cgttcttcga gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc
    51601 tttcagcgca ggcgcgggcg gtacgctgag cggcggttca tcccctttct ttatccacgc
    51661 ggtttcactg ccaacgcgct tttgctgagc atctataaac aacaacgccg cttttaagcc
    51721 actcagagaa atagtctgat tgccgtcgcg taaggtgatc gccctccctt cctgaatcat
    51781 ctgcaaaaac gcggtgatgg ttgccgtatc atcggttact aataaccatg gtgaaatccg
    51841 ccacttgtca ccacttaacg ccaacggctc accatctaac agcagccgtg gcgctatctc
    51901 cccttcagac gcatccggcg acttcaaccc gccgcgctca atacgtaaaa cggcatcggt
    51961 atgcgccccg gcgctgcggc tcagggtcat caccagtccg ttatgatcgc ccgtattacg
    52021 cgccacgcag aagttttggt tattacaggt gacctgccag tcggaaaacg cccgttgcgc
    52081 tggtgccgcc cacaccaaag acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg
    52141 atagcgcatg gacggtacga ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt
    52201 tgcgcaggta gcgcggctca atcggattta tcggattgag ccaataataa acaacgaaat
    52261 attattgtta tttttcagtc cattaaatgt gacatttgca aatagttaag caataacacc
    52321 gtcttaccgt cacgtatctc gccagttttg atcatctcca gcgccttgtt gaacggcagc
    52381 tcgagcactt caatatcttc atcttcgaca ccaccgccgg cgttagcgcg ctggctgtca
    52441 ctgtattcgg cgataaagaa gtggattagc tcagtcacac cgcctggcga catatacagt
    52501 tcaaataatt tgcgcacttc gccaacttca taacccgtct cttcaatcgc ttctttacga
    52561 atacacactt ccggttcgtc gttatccagc agcccggcgc aggtttcaat cagttgcccg
    52621 ctttcattgc cattaaccca ggtagcgacg cggaactggc gaatcagaac cacggtcttt
    52681 ttcttcgcgt tgtacaggag gatcgttgca ccattaccgc gatcgtaaac ttcacgttta
    52741 tggcggataa cttcgccatc tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa
    52801 tagttatcgg agagaatttt gtctttaatg agggtgattt gttgcgtcat accgactcca
    52861 cagcgcgaaa tgaacaatta tcttacgctg tgaagccggg tttgtctgcg caggctatca
    52921 gtgagatttg actgatttta cccccagcca gtcaataatc ccctgtgccg catgacgtcc
    52981 ttctgccatt gcggtaacca ccagatccgc accacgcacg gcgtcaccac cggcgaagat
    53041 tttcggattg gtggtctggt aacggtactg gctttccaca tccgcgatga tgcggcccca
    53101 tttgtctacc gttacaccgt gcgactccag ccacggcatc ccgtgcggat tgaagccaaa
    53161 cgccataatc accgcatcgg ctggcatgac aaattcactg ccttccaccg gcactggacg
    53221 ccgacgcccc tgggcatccg gctcgccaag acgcgtgcgc aggaaacgaa tcccgcagac
    53281 gtgaccttgt tcattcagtt caagcgccac cggctggacg ttaaattcga agttggcccc
    53341 ctcttcacgg gcgttcttca cctctttctt cgagcctggc atgttagctt catcacgacg
    53401 ataagcgcag gtgacgttac tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc
    53461 ggtgtcgccg ccgcccagta ccacgacgtt aagtccggca gtattgataa acggctcttc
    53521 cggtagctct tcgagtccca tcacctgttt agtattggcg atgaggaacg gcagcgcgtc
    53581 ataaacgccc ggcgcatctt cattgggtaa acccgctttc atggagcggt aagtgcctac
    53641 gccaacgaag accgcgtcgt attgttccaa aagcgaattc aaagagacat ctttacccac
    53701 ttcacaattg agttcgaagt gaatccccat cgcgctgaag atttcccgac ggcgtgccag
    53761 cagggattta tccagtttga aagaaggaat gccgaaagtg agcaagccac cgatttctgg
    53821 atggcgatcg tacaccgtca cctctacgcc attgcgggtc aaaacatccg cacaggccag
    53881 ccctgccgga cccgcaccga taatcgccac ccgcttgtcc actttggtga catggcttaa
    53941 gtcaggacgc caacctttcg ccaacgcctg atctgaaatg tagcgttcaa tgttaccgat
    54001 agttactgcg ccgtgctcat cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg
    54061 gcaaacgcgt ccggtaattt ccggcagggt gttggtctgg tgagaaagct cgacggcggc
    54121 gtcgatgttt ccggctttca ccagttcaat ccactgcggt atatggttat gcagcgggca
    54181 ggtccattca caaacgctgt gctcgccgca cttaaggcag cgcgaggctt cccgttgtac
    54241 ctgatcggca cggaatggca gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc
    54301 cagtttatct ggctcgccac gcgcgggcgt tgcctgcatt tgctcgactt tactcattac
    54361 cggcatttct tgcgccgcgg tactggcatg ccacggttga tgttcctgac gtgcggtacg
    54421 caagcggcgg gatttcgcca tgcctgagag tgcggcgtca gtgaccagtt gcagcgcgtc
    54481 cgctgggcaa ttctcgacac aagcaggacc gttttcgcgc cctgcacaaa ggtcgcattt
    54541 atgcgccgtg gcttttactt ttcctgccgc gacgggggtc aggacgattt gcatcgtacc
    54601 aaatggacag gccaccacgc aggatttaca gccaatacac ttttgctgat tgacctgaat
    54661 gctgtcatca acgtggctga ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc
    54721 acaatggtga caggtcacgg cactacgttg ctgctgatgt ttgataaccg taattcgggg
    54781 atgaaaatgg tgttggctca ggacatgttg ctcatcattg tgagccatga cacaggcgat
    54841 ttcacaagca tgacaaccca gacattgctg actgttggcc ataataaaac gattcataac
    54901 gaccttcttt tttggttgta aaaaccttat tctttatatg agtgttgtta ttacccgact
    54961 tacaggggaa tcggcaatgt tcatgtgccc agaataagta actatttcgc tataaactgt
    55021 ggcagatcaa ataatcccat cactgactaa attgcgtttc agcgaattgg aacattaatg
    55081 attttttgtg gagaagacgc gtgtgattgt taaacgaccc gtctcggcca gtctggcccg
    55141 ggcctttttt tacattgtgc tgctgtcgat tctttccacg ggtatcgctc tgctaacgct
    55201 ggcgagcagt ttacgcgacg ctgaggctat caatattgcc ggatcgctgc gtatgcagag
    55261 ttaccgcctg ggctacgact tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt
    55321 atttcagcag gcactgcatt caccggtatt aaccaatctc aacgtctggt atgtgccaga
    55381 ggcagtaaaa acccgctatg cgcatctgaa tgccaactgg ctggagatga ataatcggct
    55441 cagcaagggc gacttgccgt ggtatcaggc caatattaat aattatgtta atcagataga
    55501 cctgttcgtg ctggctttac agcactacgc cgaacgcaaa atgctgctgg tggtggcgat
    55561 ctccctggct ggcggcatcg gtattttcac gctggtcttt tttactttgc gccgcatacg
    55621 ccatcaggtg gttgccccgc tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg
    55681 gcagttcgac tcgccgccgc tggataccag cctgccgaat gagcttggcc tgcttgcaaa
    55741 aacctttaac cagatgtcga gcgagctgca taaattgtac cgttcgctgg aggcgtcagt
    55801 agaagaaaag acccgcgatc tccacgaggc caagcgtcgt ctggaggtgt tgtatcagtg
    55861 ttcgcaggcg ctaaacacca gccagattga tgtgcattgt ttccgccata ttttgcagat
    55921 tgttcgcgac aatgaagcgg ctgaatatct ggagttaaat gtcggtgaca actggcggat
    55981 tagcgaaggg cagccaaacc cggaattgcc gatgcagatt ttaccggtga caatgcaaga
    56041 gacggtttac ggcgaactgc actggcaaaa tagtcacgtt tcatcatcag aaccgctgct
    56101 taacagtgtt tcgtcgatgc tgggacgcgg tttgtacttt aatcaggcgc agaagcattt
    56161 tcagcagtta ttgttgatgg aagaacgtgc gaccatcgcc cgcgaattgc acgactcgct
    56221 ggctcaggta ctttcttact tacgtatcca gttgacgtta ctgaagcgtt cgataccgga
    56281 agataacgcc accgcacaaa gtatcatggc cgatttctcc caggcgctga atgatgctta
    56341 tcggcagtta cgcgagctgt taactacttt ccgcctgacg ctgcagcagg cggatctccc
    56401 ctccgcgttg agggaaatgc tggatacgtt acaaaatcaa accagcgcca aactgaccct
    56461 cgactgccgt ctgccaaccc tggcgctgga tgcgcaaatg caggtgcatt tgttgcaaat
    56521 tattcgcgaa gcggtgctga atgcgatgaa gcacgccaac gccagcgaaa tcgccgttag
    56581 ctgcgtcacc gcgccggatg gcaatcacac agtctatatt cgcgacaacg ggattggcat
    56641 cggtgaaccg aaagaacccg aaggccatta tggtctgaat atcatgcgcg aacgcgcgga
    56701 aagattgggt ggaacgctga ctttttcaca accttccggc ggcggcacgt tagtgagtat
    56761 tagctttcgc tctgcggagg gtgaggaaag tcagctgatg taatgcctct tattgaccaa
    56821 agaatagtgg cacttaaggt tcagtataaa agggcatgat aatttacatt aactcctttt
    56881 tttctccacg attggctcgt accttgccgc tacagtgaag caagtcaagc ctacaacgat
    56941 acgcagaaac acgaggtcct cttttaatgg cgaatttctt tattgatcgc cccatttttg
    57001 cctgggtgct ggcaatcctg ttgtgtctga caggtaccct ggcgattttt tcattgcccg
    57061 ttgaacaata ccccgatctc gcgccaccga atgtgcgagt gaccgctaac tatcccggcg
    57121 catcggccca gacgctggaa aacaccgtga cccaggttat cgagcaaaat atgaccggcc
    57181 tcgataatct catgtatatg tcgtctcaga gcagtggcac cggccaggca tctgtcactt
    57241 taagttttaa agcaggcacc gatccggacg aagccgtgca gcaagtacaa aaccagctgc
    57301 aatcagccat gcgaaagtta ccgcaggcgg tgcaaaatca gggcgtgacg gtgcgtaaaa
    57361 ccggcgatac caatattctg accattgcct tcgtctctac cgatggttcg atggataaac
    57421 aggatattgc cgattacgtt gccagtaata ttcaggaccc gttaagccgc gtgaatggcg
    57481 tcggggatat cgatgcctat ggttcgcaat attccatgcg tatctggctg gacccggcga
    57541 aactcaacag tttccagatg acggctaaag atgtcactga tgccattgag tcacagaacg
    57601 cgcagattgc ggttgggcaa cttggtggta cgccttccgt cgataagcag gcgctcaacg
    57661 ccaccattaa cgcccagtca ctgctgcaaa caccagaaca gttccgcgat atcaccttgc
    57721 gggtgaatca ggacggttca gaggtaaggc tgggcgatgt cgccaccgtc gaaatggggg
    57781 cggaaaaata cgattatctt agccgcttca atggtaagcc agcctccggg ctgggggtaa
    57841 aactggcctc cggcgcgaac gaaatggcga ctgcgcagct ggtgctcaat cgtctcgacg
    57901 agctggcgca gtattttcca cacggactgg aatacaaggt ggcgtatgaa accacctcgt
    57961 ttgttaaagc ctccattgaa gacgtggtga aaacgctgct ggaagctatc gctctggttt
    58021 tcctcgttat gtatctgttc ctgcaaaact tccgcgccac gctgataccc actatcgccg
    58081 tgccggtggt gttgatggga accttctccg tactttatgc cttcggttac agcgtcaaca
    58141 ccttaaccat gttcgcgatg gtgctggcga tcggtctgct ggtggatgat gccatcgtgg
    58201 tggtggaaaa cgtcgaacgt attatgagtg aggaaggact cactcctcgc gaagccacac
    58261 gtaaatcgat ggggcagatc cagggggcac tggtcgggat tgcgatggtg ctttcggcgg
    58321 tatttgtgcc aatggccttc ttcggcggca ccaccggtgc catctatcgc cagttctcta
    58381 ttaccattgt tgcggcgatg gtgctgtcag tactggtagc gatgatcctc actccggctc
    58441 tgtgtgccac gctacttaag ccactgaaaa aaggtgagca tcacgggcaa aaaggctttt
    58501 ttgcctggtt taaccagatg tttaaccgca acgccgaacg ctacgaaaaa ggggtggcga
    58561 aaattctcca ccgtagcctg cgctggattg tgatttatgt cctgctgctt ggcggcatgg
    58621 tgttcctgtt cctgcgtttg ccgacgtcgt tcttgccact tgaagaccgt ggcatgttta
    58681 ctacctcggt acagttgccc agcggttcaa cccaacaaca gaccctgaaa gtcgttgagc
    58741 aaatcgagaa atactacttc acccatgaaa aagacaacat catgtcggtg tttgccactg
    58801 ttggttctgg ccctgggggt aacggacaaa acgtggcgag aatgtttatc cgcctgaaag
    58861 actggagcga acgcgacagt aagaccggca cctcgtttgc cattatcgag cgtgcaacga
    58921 aggcgtttaa caagattaaa gaagctcgcg ttatcgccag cagcccgcca gcaattagcg
    58981 gtcttggtag ttctgcaggt tttgatatgg agttgcagga ccacgctgga gcgggtcacg
    59041 atgcgctgat ggcagcacgt aaccagttgc tggcgctggc ggcggaaaac ccggagctaa
    59101 cccgtgtgcg ccataacggc ctcgacgaca gtccgcagtt gcagattgat atcgaccagc
    59161 gtaaagctca ggcgctgggc gttgctatcg acgatattaa cgacacactg caaaccgcct
    59221 ggggttcgag ctatgtgaat gactttatgg atcgcggtcg cgtgaagaaa gtctatgtgc
    59281 aggcagctgc gccgtatcgc atgctgccag atgacatcaa tctctggtat gtccgaaata
    59341 aagatggcgg catggtgccc ttctctgctt tcgcgacctc acgctgggag acaggctcgc
    59401 cgcgtctgga acgctataac ggttattctg cggtagagat tgtaggggaa gccgcaccgg
    59461 gggtcagtac cggtacggcg atggatatta tggaatcgtt agtgaagcag ttgccaaacg
    59521 gctttggtct ggagtggacg gcgatgtcgt atcaggagcg gctttccggc gcgcaggctc
    59581 cggcgctgta cgccatttcc ttgctggtgg tattcctgtg tcttgcggcg ttgtatgaaa
    59641 gctggtcggt gccgttctcg gtaatgctgg tcgtgccgct gggggttatc ggcgcgctgc
    59701 tggcaacctg gatgcgcggg ctggaaaacg acgtttactt ccaggtgggc ctgttaacgg
    59761 tcattggttt atcggcgaaa aacgccatcc tgatcgtcga gtttgctaac gagatgaacc
    59821 aaaaaggcca cgacctgttt gaagcgacgc tccacgcctg ccgtcagcgt ttacgcccga
    59881 ttctgatgac ttcgctggca tttatcttcg gcgtattgcc aatggcaacc agcacgggtg
    59941 ccggttccgg tggtcagcat gcggtgggta ctggcgtaat gggcgggatg atttcggcca
    60001 ctattctggc tatttacttc gtgccgctgt tctttgtgct ggtgcgccgc cgcttcccgc
    60061 tgaagccgcg cccggaataa gcaataaaaa aggcgacatg ctaatgtgtc gcctttttca
    60121 actttccgat taagaacctg ctcagcgggt tcttgctgtt tgtactttgt ctcaggaatt
    60181 acttacgaag cataacttcg ataaagtctt tccagttccc cagttcacgt tcaatcataa
    60241 caacctctct tataattatg ggtattctac ggaaacaata taccgtggtg aagctaattt
    60301 actcgattgc tgcgatgact acctccgggg gacaaacctt atgtaaatac tatggtcctg
    60361 cggtgataat ttgtatgtga tacacagcaa catttcgaga
//
LOCUS       DAZUCC010000004        54535 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZUCC010000004 DAZUCC010000000
VERSION     DAZUCC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.004"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            197..349
                     /gene="hokA"
                     /locus_tag="ACHY09_000175"
     CDS             197..349
                     /gene="hokA"
                     /locus_tag="ACHY09_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFS3883199.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMVRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(537..749)
                     /gene="cspA"
                     /locus_tag="ACHY09_000176"
     CDS             complement(537..749)
                     /gene="cspA"
                     /locus_tag="ACHY09_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFS3883200.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1030..1320)
                     /locus_tag="ACHY09_000177"
     CDS             complement(1030..1320)
                     /locus_tag="ACHY09_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFS3883201.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1754..2464
                     /locus_tag="ACHY09_000178"
     CDS             1754..2464
                     /locus_tag="ACHY09_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFS3883202.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2514..3488)
                     /gene="ghrB"
                     /locus_tag="ACHY09_000179"
     CDS             complement(2514..3488)
                     /gene="ghrB"
                     /locus_tag="ACHY09_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFS3883203.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTIEQNAAILA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3592..4251)
                     /locus_tag="ACHY09_000180"
     CDS             complement(3592..4251)
                     /locus_tag="ACHY09_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFS3883204.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4404..6737
                     /gene="bisC"
                     /locus_tag="ACHY09_000181"
     CDS             4404..6737
                     /gene="bisC"
                     /locus_tag="ACHY09_000181"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFS3883205.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVMVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6706..7146)
                     /locus_tag="ACHY09_000182"
     CDS             complement(6706..7146)
                     /locus_tag="ACHY09_000182"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFS3883206.1"
                     /translation="MIREVQHSELPAILELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7143..7706)
                     /gene="tag"
                     /locus_tag="ACHY09_000183"
     CDS             complement(7143..7706)
                     /gene="tag"
                     /locus_tag="ACHY09_000183"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS3883207.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7864..8562
                     /locus_tag="ACHY09_000184"
     CDS             7864..8562
                     /locus_tag="ACHY09_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFS3883208.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTIGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8791..9999
                     /locus_tag="ACHY09_000185"
     CDS             8791..9999
                     /locus_tag="ACHY09_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3883209.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10323..12014
                     /gene="eptB"
                     /locus_tag="ACHY09_000186"
     CDS             10323..12014
                     /gene="eptB"
                     /locus_tag="ACHY09_000186"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFS3883210.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            12106..12182
                     /locus_tag="ACHY09_000187"
     tRNA            12106..12182
                     /locus_tag="ACHY09_000187"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12140..12142,aa:Pro,seq:cgg)
     gene            12217..12350
                     /locus_tag="ACHY09_000188"
     ncRNA           12217..12350
                     /ncRNA_class="other"
                     /locus_tag="ACHY09_000188"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            13095..14702
                     /gene="dppA"
                     /locus_tag="ACHY09_000189"
     CDS             13095..14702
                     /gene="dppA"
                     /locus_tag="ACHY09_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFS3883211.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(14765..14992)
                     /locus_tag="ACHY09_000190"
     CDS             complement(14765..14992)
                     /locus_tag="ACHY09_000190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883212.1"
                     /translation="MTRSLELRVCIAHKSIPALVEQGMKYCCRMALHAEAQRHRVCRL
                     LTKSALFMPDAAQTPYPTYKIVQTQYIAEFS"
     gene            15010..16029
                     /gene="dppB"
                     /locus_tag="ACHY09_000191"
     CDS             15010..16029
                     /gene="dppB"
                     /locus_tag="ACHY09_000191"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFS3883213.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16039..16941
                     /gene="dppC"
                     /locus_tag="ACHY09_000192"
     CDS             16039..16941
                     /gene="dppC"
                     /locus_tag="ACHY09_000192"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFS3883214.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16952..17935
                     /gene="dppD"
                     /locus_tag="ACHY09_000193"
     CDS             16952..17935
                     /gene="dppD"
                     /locus_tag="ACHY09_000193"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFS3883215.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17932..18936
                     /gene="dppF"
                     /locus_tag="ACHY09_000194"
     CDS             17932..18936
                     /gene="dppF"
                     /locus_tag="ACHY09_000194"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFS3883216.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18966..20237)
                     /locus_tag="ACHY09_000195"
     CDS             complement(18966..20237)
                     /locus_tag="ACHY09_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFS3883217.1"
                     /translation="MQHNTLPKHDQKMPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20713..20820
                     /locus_tag="ACHY09_000196"
     CDS             20713..20820
                     /locus_tag="ACHY09_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS3883218.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWLNKRK"
     gene            21196..21303
                     /locus_tag="ACHY09_000197"
     CDS             21196..21303
                     /locus_tag="ACHY09_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS3883219.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21679..21786
                     /locus_tag="ACHY09_000198"
     CDS             21679..21786
                     /locus_tag="ACHY09_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS3883220.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            22162..22269
                     /locus_tag="ACHY09_000199"
     CDS             22162..22269
                     /locus_tag="ACHY09_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS3883221.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22356..24035)
                     /gene="bcsG"
                     /locus_tag="ACHY09_000200"
     CDS             complement(22356..24035)
                     /gene="bcsG"
                     /locus_tag="ACHY09_000200"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFS3883222.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDITEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(24032..24223)
                     /gene="bcsF"
                     /locus_tag="ACHY09_000201"
     CDS             complement(24032..24223)
                     /gene="bcsF"
                     /locus_tag="ACHY09_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFS3883223.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(24220..25791)
                     /gene="bcsE"
                     /locus_tag="ACHY09_000202"
     CDS             complement(24220..25791)
                     /gene="bcsE"
                     /locus_tag="ACHY09_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFS3883224.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            26064..26252
                     /gene="bcsR"
                     /locus_tag="ACHY09_000203"
     CDS             26064..26252
                     /gene="bcsR"
                     /locus_tag="ACHY09_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFS3883225.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            26264..27016
                     /gene="bcsQ"
                     /locus_tag="ACHY09_000204"
     CDS             26264..27016
                     /gene="bcsQ"
                     /locus_tag="ACHY09_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFS3883226.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICTGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            27013..29631
                     /gene="bcsA"
                     /locus_tag="ACHY09_000205"
     CDS             27013..29631
                     /gene="bcsA"
                     /locus_tag="ACHY09_000205"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFS3883227.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            29642..31981
                     /gene="bcsB"
                     /locus_tag="ACHY09_000206"
     CDS             29642..31981
                     /gene="bcsB"
                     /locus_tag="ACHY09_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFS3883228.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            31988..33094
                     /gene="bcsZ"
                     /locus_tag="ACHY09_000207"
     CDS             31988..33094
                     /gene="bcsZ"
                     /locus_tag="ACHY09_000207"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFS3883229.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWALLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            33076..36567
                     /gene="bcsC"
                     /locus_tag="ACHY09_000208"
     CDS             33076..36567
                     /gene="bcsC"
                     /locus_tag="ACHY09_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFS3883230.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQAGQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQW
                     SSNIQELVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRD
                     YTAARAAYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRR
                     VALAQAQLGDTAAAQQTFNKLIPLAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETY
                     KDAMVASGVTTTRPLDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDY
                     WGSSGTGGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGT
                     CTLQDCSGNRSQSDSGASVAVGWRNDTWSWDIGTTPMGFNVVDVVGGISYSDDIGPLG
                     YTVNAHRRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLS
                     GDQLTGKNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGY
                     YSPQEYLSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAA
                     RQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGW
                     QGDMDLPPQPLIPYADW"
     gene            36649..38637
                     /gene="hmsP"
                     /locus_tag="ACHY09_000209"
     CDS             36649..38637
                     /gene="hmsP"
                     /locus_tag="ACHY09_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFS3883231.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            38820..40106
                     /gene="dctA"
                     /locus_tag="ACHY09_000210"
     CDS             38820..40106
                     /gene="dctA"
                     /locus_tag="ACHY09_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFS3883232.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            40327..41823
                     /locus_tag="ACHY09_000211"
     CDS             40327..41823
                     /locus_tag="ACHY09_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS3883233.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(41919..42848)
                     /gene="kdgK"
                     /locus_tag="ACHY09_000212"
     CDS             complement(41919..42848)
                     /gene="kdgK"
                     /locus_tag="ACHY09_000212"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFS3883234.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSQTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            43080..43847
                     /gene="pdeH"
                     /locus_tag="ACHY09_000213"
     CDS             43080..43847
                     /gene="pdeH"
                     /locus_tag="ACHY09_000213"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFS3883235.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            43917..45977
                     /locus_tag="ACHY09_000214"
     CDS             43917..45977
                     /locus_tag="ACHY09_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFS3883236.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLASDDNKDTNAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(46156..47478)
                     /locus_tag="ACHY09_000215"
     CDS             complement(46156..47478)
                     /locus_tag="ACHY09_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3883237.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(47871..48884)
                     /gene="yhjD"
                     /locus_tag="ACHY09_000216"
     CDS             complement(47871..48884)
                     /gene="yhjD"
                     /locus_tag="ACHY09_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFS3883238.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(48933..49832)
                     /locus_tag="ACHY09_000217"
     CDS             complement(48933..49832)
                     /locus_tag="ACHY09_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3883239.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            50352..50954
                     /locus_tag="ACHY09_000218"
     CDS             50352..50954
                     /locus_tag="ACHY09_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS3883240.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(51005..52654)
                     /locus_tag="ACHY09_000219"
     CDS             complement(51005..52654)
                     /locus_tag="ACHY09_000219"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFS3883241.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKACARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            53059..54456
                     /gene="ccp"
                     /locus_tag="ACHY09_000220"
     CDS             53059..54456
                     /gene="ccp"
                     /locus_tag="ACHY09_000220"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFS3883242.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT        13090 a        13576 c        14757 g        13112 t
ORIGIN      
        1 gattatatca aggagaagaa acgaacttat taagctagaa tagctgtggg tgcttgagac
       61 tgtttgtctc aggtattcac cgaaaggcag acagagaaaa gccccacctg actataaatc
      121 aaagtgaggc taccctatgc ctgaacacca taaggttagc ctcttactcg ttggaaatca
      181 acacaggagg ctgggaatgc cgcagaaata tagattgctt tctttaatag tgatttgttt
      241 cacgctttta ttttttacct ggatggtaag agattcactg tgtgaattgc atattaaaca
      301 gggaagttat gagctggcgg catttttagc ctgcaatttg aaagagtaag agactttggc
      361 gggaagttat tcccgccttt cttacggcgt tgcgcattct cattgcaccc aaatttattc
      421 ttcacaaaaa taacactaga ttctattacg cgatcgatta tttatttcct gaaaaacaaa
      481 taaaaaaatc cccgccaaat ggcagggatc ttagattctg tgcttttaag cagagattac
      541 aggctggtta cgttaccagc tgccgggcct ttagcgccgc tttcgatggt gaaggacact
      601 ttctgacctt cgtccagaga tttgtaacca tcgttctgga tagcagagaa gtgtacgaac
      661 acgtctttag agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat
      721 tttacgatac cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct
      781 ttcggcgata tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg
      841 gtatcaacga taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt
      901 caaaccgttg atgtgcatta agccacgcat tggcgggtga tgcaacaatt atttttcata
      961 tttatgatta atcggttgga aaacggtcct gtcatcagga ccgtaaacag caataaagtg
     1021 gataaaagtc tattccatca actgcttact taatgccggg ttggcttgaa tcaaacgcat
     1081 caattttagt tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc
     1141 gactgatacg cccaaaaccc gggcgaaatc atcgattttt aaccctgtcc cttttcgtaa
     1201 ctgctcaatt tcggtacagg acgttgttct gtgcgtcagg gtaattttct gcgtttcatc
     1261 tttaaaaaca atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat
     1321 tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag
     1381 tcgggaaaaa cagacatagc ggggtgtagt cagggcatgc tctggctatg attcgattca
     1441 gccagtcatt tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct
     1501 taccactccc ggtttgtaaa gatttcgaaa atctaacgtt ctgcgaaggc gtaaatctca
     1561 ggctacaaaa aaagcaacgg cactatcatt ttttaacaat gaaacaacaa attgaccttg
     1621 tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg
     1681 tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga
     1741 aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc
     1801 gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc
     1861 gtttatcgac ttcctgcaga atacagtgat gcgtagcggt gaacgtctac caaccctgac
     1921 tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta
     1981 ttctcagcag gtgaatcagg cgatggattc cggtctgcgc ccggttgtag acagcgttaa
     2041 cgcaattcgc gtaccgcagg attacgttac gcaaagcggc ccactgcgtg aaatgaacgg
     2101 ttctctgggc gtactggcgc aacagctgca aaatgcgaag ttgcaggccg atgctgcgca
     2161 ctctgcgcta aaacagagcg atgacctgaa accggtcttc gatcaggcat tcaccaaagt
     2221 ggtaacgacg ccagctgatg cgttgcaacc gttgattccg gcggcacaaa ctttcacgca
     2281 acaactggtg atggttggag actacattgc tcagcaaggt actcaggtga gctttgtcgc
     2341 aaatggcatc cagttcccga cttcgcagca ggcaagtgaa tataacaaac tgattgcgcc
     2401 attaccggca caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca
     2461 ataaagagta aaagcccgag cgattatcgg gctttactcc acgcagtcgc ggcttagtcc
     2521 gcgacgtgcg gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaagtta
     2581 tccacggcac aggcggccat gccataacgc gtctcatgag tggcagatcc aatatgcggt
     2641 actgcgacga cgttggccat tgagagcaac ggcgaatcta ctgacagtgg ctcttgttcg
     2701 aagacatcca gcccggcagc gtgaatttcc cctttctgca atgcagcaat cagtgcattt
     2761 tcatcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg
     2821 aattgttctg cgccaaacag atgatgcgtt tcatcagtta acggcaggat caggcaaacg
     2881 aaatctgact cttgtaacag tgtatccaaa tcgcagtagc gggcgttgaa gcgttcttct
     2941 gcttctttat ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcgca
     3001 cgttgtgcca gcgccatgcc gatccgtccc atcccgacaa tgcccagtgt tttatggtga
     3061 acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttactcgt
     3121 tctgctacct ccacaacccg acgagcggta gacaatacca gcgccatcag cgtatcggcg
     3181 acggtttctg ttaatacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca
     3241 tcgacatcaa aattgtcata gcctacggag atcgttgatg tggcacgcag tttcggcatt
     3301 ttttccagca atgcggcatc aacattctcg tttgaaccca gtaaaccttc agcttcggca
     3361 agaattgctg cattttgttc gatggtttgt gggctgaggt ttgccacctg gtgaacggtg
     3421 aaatgctctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac
     3481 ggcttcattc ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa
     3541 ggaaaactga agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg
     3601 gcttaaggta atttctacac ggcggttttg cgccttacct tctgcagtgc tgttgctggc
     3661 gattgggtta gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt
     3721 gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcaggtcgtg
     3781 cccacccgtg ctgtcggtat aaccaatcac gttaaccgcc gttttcggat actctttcag
     3841 taccattgcc acgccggtca gggtgttagc gcccgccggt ttcagggttg cgctgctgct
     3901 gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct
     3961 aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc
     4021 cacgccgccg cccagagctg cgcctgctgc tgcgccaatg agcgcgcctt taccgcgatc
     4081 tttcttcgaa gaagagagcg caccaatacc cgcgccaacg agagagccca gacctgcgcc
     4141 gatagcagat ttacctgctt cgcgttcgcc ggtgtaaggg ttagttgtac agccagatac
     4201 cgccagagcg ccactcacta cggcggcaat aagataaaca cgtttcttca ttgttaatcc
     4261 ttaataacct ttttattctt tgccacgggt tctgtggcgg gagattatgc cgcgtgaaca
     4321 tgaagattat tcctgggaat actcggaaat ttgtaagtaa tatttaactg ctcaatacat
     4381 ctaacctttc aggagtcttc ggtttggcca actcatcctc acgatattcc gttctgactg
     4441 ccgcccactg ggggcctatg ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg
     4501 gcgcgttagc cacaggaatg gaaaactcct tgcagagcgc ggttcgcgac caggttcaca
     4561 gcaatacgcg ggtgcgattt ccaatggtgc gcaaaggctt tcttgcgtca ccggaaaatc
     4621 cgcaaggcat tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag gcgctggagc
     4681 ttattcacca tcaacataaa cgcattcgtg aggcttatgg tccggcatcg atttttgctg
     4741 gttcctacgg ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct
     4801 atatggcgct ggcaggcggt tatactgggc atctggggga ttattcgacc ggcgcggcac
     4861 aggcgatcat gccgtatgtc gtgggtggca gcgaagttta tcaacagcaa accagttggc
     4921 cgctggtgct ggaacatagc gatgtcatgg tgctgtggag tgctaaccca ctcaatacgc
     4981 tgaaaattgc gtggaatgca tccgatgagc aggggctttc ttacttttcc gcactgcgtg
     5041 acagcgggaa aaagctgatc tgcattgatc caatgcgatc ggaaaccatc gatttctttg
     5101 gcgataaaat ggagtgggtg gcaccacaca tgggcaccga tgttgcgttg atgctgggga
     5161 tcgcccatac gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca
     5221 caggttatgc cgtcttcgcc tcttatttgc tgggcgagag tgatggtata gcgaaaaacg
     5281 ccgaatgggc ggcagagatt tgtggtgttg gcgcagcgaa aatccgcgag ctggcggcta
     5341 ttttccacca aaatacaacc atgctgatgg caggctgggg aatgcagcgc caacagtttg
     5401 gtgagcaaaa acactggatg ctcgtcacgc tggcggcaat gttggggcaa atcggcacac
     5461 ccggcggcgg ttttggtctt tcttaccatt ttgccaatgg tggtaacccc acgcgccgcg
     5521 ctgcggtgct ctcttccatg cagggcagtt tgcctggcgg caccgatgcg gtggataaaa
     5581 tccctgttgc ccgcattgtt gaagcactgg aaaaccccgg tggcgcatat caacataacg
     5641 gtatggaccg acatttcccg gatattcgtt ttatctggtg ggcgggtggt gccaacttta
     5701 ctcatcatca ggataccaat cgcctgatcc gtgcctggca aaaaccggag ctggtggtga
     5761 tctctgaatg cttctggacg gcggcggcga aacacgcgga tatcgttctg cctgcgacca
     5821 cctcttttga gcgtaatgat ctcaccatga ccggcgatta cagtaatcag catctggtgc
     5881 cgatgaagca agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg
     5941 agttaagtga acgttgggag aagggcggct atgcgcggtt tacggaagga aaaagtgagc
     6001 tgcaatggct ggaaacgttt tataacgttg cccggcaacg cggggcaagc cagcaggttg
     6061 aattgccgcc atttgctgag ttctggcaag ccaaccagtt aattgagatg ccggaaaacc
     6121 cggacagcga gcggtttatt cgcttcgccg attttcgccg cgatccgcag gcgcatccat
     6181 taaaaaccgc cagcggcaag attgaaatct tctcgcagcg tattgccgat tacgcttatc
     6241 cggattgccc tgggcatcca atgtggctgg agccggacga atggcagggc aatgccgaac
     6301 cggaacagtt gcaggtactt tctgctcatc cggcacatcg tctgcacagc cagctgaatt
     6361 acagttctct gcgcgaattg tacgcggtgg caaaccgtga gcctgtcacc attcatcctg
     6421 acgatgctca ggcgcgcggc atacaagatg gcgatactgt tcggctgtgg aacgcacgcg
     6481 ggcaaattct tgccggggca gtcattagcg agggaattaa acctggcgtg atttgcattc
     6541 acgaaggggc atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag
     6601 tgaacgtgct gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata
     6661 cggcgctggc atggctggaa aaatacaacg gtccggaact gacacttaca gcgtttgaac
     6721 caccggccag ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcagtcgac
     6781 aatgtgaaaa ccctgcgcct ggtaaaaatc tatcgccggt tgattttttt gataaacctc
     6841 cagcatcaga tggggatggc gctgctgcac atactgcatt agcgccttac caataccgcg
     6901 tctgacggcc ttcggtgcga caaacattgc agccagaaat cggccttcca taatgctgac
     6961 aaaaccgaga agcttaccgt cttcttccca gacccagttt tgcgcgttgg caagatacgc
     7021 atcgcgcacc agcggaatgc agtcacgcca gtaactcgat tttataaagg gatgccccca
     7081 ggttgtactt tccagccaca gttcgaggat cgcggggagt tctgaatgtt gcacttcccg
     7141 aatcatggtt tatttcccgg atggcaacag cagccaacca catgatcatt caccagccca
     7201 catgcctgca taaaggagta acagattgtg gtaccgacaa acttaaaacc acgttttttt
     7261 agtgccttag atagggcgtc ggaggcgggt gtagatgtgg ggatttcgct caacgttgtg
     7321 gcttgtgtca cctgtggttg atgattgaca aacgaccaga caaagtcggc aaacggttcg
     7381 ccgttctgtt ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttcccg
     7441 cgatggcgga taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgca
     7501 gccaccttca ccggatcgaa ctgatgaaag ctggcgcgat agttttcgcg ttttttgagg
     7561 acggtgatcc acgataatcc agcctgctgc ccttcaaggc agatcatttc gaacagtttt
     7621 ttactgtcag tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggtcc
     7681 tgactcaccc agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt
     7741 tcagccgctt ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc
     7801 ccgatatcct tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg
     7861 ttaatgatca taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagtgtggta
     7921 atcagtgcct tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg
     7981 caacccggac attccacaga gcgttacacc tgggatagcg atcatcaacc tgattacaac
     8041 gatattttgt cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg
     8101 gcggaagaaa ctcctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta
     8161 tatgagcagg ttacaatcgg cccggtcgcg gcattacatt acgatggcac aaccacctcg
     8221 atgtataacg agtttggcga cagtactacc acgctggccg atccgttatg gcatgccagc
     8281 gtgagtacct taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa
     8341 atcagctata accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg
     8401 atgacggcga caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg
     8461 ctcaatcaaa atattgctgc ctatgctgcg ctatctcagg cagaaaatac cactaacaat
     8521 agcgactatc tgtatacgat gggggttagc gccagatttt aacgttatag atacaattga
     8581 aaccaataaa taacaactaa tgtggcgata gtgggtgata acttactaaa taataaattt
     8641 ggtgaataat tgtcgagtca ttcattcctg aactaatgca tttcattccg ttctgatggc
     8701 atttcatgcc gtttttcccc aggcataaag tgcacttcgt tatggttgtc agcagagatt
     8761 tttccttttt attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc
     8821 tggttgacac tcatcggtac tatcattacc cagtttgccc ttgggtcggt ttatacctgg
     8881 agcctgttta acggcgcgct ttctgccaag ctggatgcgc cggtaagcca ggtcgctttc
     8941 tctttcggct tgttaagtct ggggctggca atttcatctt ctgttgcggg gaaattacag
     9001 gaacgttttg gcgttaaacg cgtcactatg gcttccggca ttttgctggg attaggcttc
     9061 ttcctgacag cgcattctga caacctgatg atgctgtggt taagcgccgg tgtgctggtc
     9121 ggtctggcgg atggcgcggg ttacctgctg acgctctcta actgcgtgaa gtggttcccg
     9181 gagcgtaaag gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt
     9241 ttcaaattta tcgacacgca gctgctggaa acggtgggtc tggaaaaaac ctttgtgatt
     9301 tggggagcga ttgcgctggt gatgattgtc tttggcgcaa cattaatgaa agacgcacca
     9361 aaacaggaag tgaaaaccag caatggtgtg gtggagaagg actacaccct ggcagagtcg
     9421 atgcgtaaac cgcagtactg gatgttagcg gttatgttcc tgactgcgtg catgagtggt
     9481 ctgtatgtga ttggtgtagc gaaagatatc gctcaaagtc tggcgcatct tgatgcaatt
     9541 tccgcagcca atgctgtgac ggttatttcc atcgccaacc tttctggtcg tctggtgctg
     9601 ggcattctgt ctgacaaaat cgcccgtatc cgtgttatca ccattggtca ggtgatatcg
     9661 ctggtgggta tggcggccct gctgtttgca ccattgaatg cagtgacgtt ctttgcagcg
     9721 attgcctgcg tggcatttaa ctttggcggc actatcacgg tgttcccgtc actggtcagt
     9781 gagttcttcg gcctcaataa cctggcgaaa aactacggtg tgatttatct cggtttcggt
     9841 atcggcagca tttgtgggtc gattatcgcc tcactgtttg gcggcttcta tgtgaccttc
     9901 tacgtgattt tcgcactgct gattctgtca ctggcgctct ctacgacgat tcgtcagcca
     9961 gagcagaaaa tgttgcgtga ggcgcatggc tccctttaat catctgccgc gagctactgg
    10021 gctttagcct tagtgtgaat caaacgcttc aacgcaatgt ataaacaatg acgcgtaaaa
    10081 tttgggccag ttaactggcc ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa
    10141 cttgcatttt tgtaaatttg tgcttcatgc acactctttc cccacacttt ttccctttgc
    10201 tgtggtctac ttattcgcgc gtgtagattt tacttatctg actacctccg cacttttccc
    10261 ctgccgggcc tgaaaagccg ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt
    10321 gcatgagata catcaaatcg attacacagc agaagctgag ctttttgctt gcaatctata
    10381 ttggcctttt tatgaatggc gcggtttttt accgccgctt cggcagctat gcgcacgatt
    10441 ttaccgtctg gaaaggcatt tctgctgttg ttgaactggc cgccaccgta ctggtgacct
    10501 tctttttact acgtcttctt tcgctgtttg gccgccgtag ctggcgtatt ctggcgtcgc
    10561 tggtggtgct cttttccgca ggtgccagct attacatgac cttccttaat gtggtcattg
    10621 gttatggcat catcgcttcc gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg
    10681 gtctgaactt tattctctgg ttaatcgccg ttagcgcgtt gcctcttatc cttatctgga
    10741 ataaccgctg tcgttacacc ttgctccgac aactgcgaac cccagggcag cgtattcgca
    10801 gcctggcggt cgtcgtactg gcaggtatta tggtttgggc accgattcgt ttgctggata
    10861 tccagcagaa gaaagtggag agggcgacag gtgttgattt gccgagttat ggcggcgtcg
    10921 tcgcgaactc ttatctgcca tcaaactggc tttcggcatt aggtctttat gcctgggcgc
    10981 gggtcgatga atcttccgat aacaattctc tccttaaccc tgcgaagaag tttacgtacc
    11041 aggctccaca aaacgttgat gatacatatg tcgttttcat tattggtgaa accacgcgct
    11101 gggatcatat gggcattttc ggctatgaac gcaataccac accgaagctg gcccaggaaa
    11161 aaaatctggc ggcgttccgt ggttactcct gtgataccgc aaccaaactc tctctgcgtt
    11221 gcatgtttgt acgtcagggg ggcgcggaag ataatccgca gcgcacatta aaagaacaga
    11281 acattttcgc ggtattgaag cagttaggat tcagttctga tctctacgct atgcaaagcg
    11341 aaatgtggtt ctacagcaat acgatggcgg acaacattgc ttatcgtgag cagattggtg
    11401 cggagccacg taaccgtggc aaaccggtag atgatatgtt gctggtagac gaaatgcagc
    11461 aatcgctggg gcgcaacccg gatggtaagc atctgatcat tctgcatacc aaaggctcac
    11521 actttaacta cacccagcgt tacccgcgca gctttgcgca gtggaagccg gaatgtattg
    11581 gtgttgatag cggctgtacc aaagcgcaga tgatcaactc atacgacaac tcggtgacct
    11641 atgtggatca ctttatttcc agcgtgattg atcaggttcg cgataagaaa gcgattgtgt
    11701 tctatgcagc tgaccatggt gagtcaatta atgaacgcga gcacctgcac ggcacgccgc
    11761 gtgaactggc accgccggaa cagttccgcg tgccgatgat ggtctggatg tcggataaat
    11821 atctggaaaa tccggtcaat gcgcaggcat ttgcgcagct gaaaaaagaa gctgacatga
    11881 aagtgccacg ccgtcacgta gagctgtacg acaccatcat gggttgtctt ggctatactt
    11941 caccggatgg tggaattaac gaaaacaaca actggtgcca catcccgcag gcaagagagg
    12001 cggcggctaa ctaagaggcc tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa
    12061 gggattgacg agggcgtatc tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag
    12121 cctggtagcg cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac
    12181 caaattcgaa aagcctgctc aacgagcagg cttttttgca tttgtagcct atgaggatga
    12241 gaacctccgg gggcagggag gttcgactcg agcgaagcga gagaacgttg cgccagcaac
    12301 ggcccgcagg gcgagccacg aagtggcgag taatcctcta tcaccgacca aattcgaaaa
    12361 gcctgctcaa cgagcaggct tttttgcatc tgtagctcat gaggaagaga acccccgggg
    12421 gcggggaggt tcgactcgac gtaggtctga taagacgcgc cagcgtcgca tcaggcaacg
    12481 gcaccgcact tattgtcgga tgcggcgtga acgccttatt cgatctacac accacacatt
    12541 tccctgtcaa atttgcgccc catatctcac tatccattaa cggatttgtg acagaaacca
    12601 ttgtcttttt ttatatttgc ttaaatcttt ttttgcgtta cttatggctg gcgctggcat
    12661 tttccgctgt gcgttttagc gttcatggta ttaattgctc agatatccac cttttcctca
    12721 gatttttttc attaaatgca taaaacttaa tcaccaaatg ttgctaaata ttaagctgat
    12781 tgttctggtt gtgttggcgt agcacaaatt tctctgctgc aaatagctgt ttgaagtttt
    12841 tttaatcttt gtttgtggat tctcaccgtt ggtgtaaatc ccggttggct gtattgacgt
    12901 tttcacattc tgttgacaga ttgtaggtca cgaggggcat tttatggagg atccgcactg
    12961 ttacactgat gttaattagt acggcatccc cacctcataa cgttgacccg accgggcaaa
    13021 aaacaaaaaa ggtcaggcag cgacaaccca ctgcaaaggg ttaaaacaac aaacatcaca
    13081 attggagcag aataatgcgt atttccttga aaaagtcagg gatgctgaag cttggtctca
    13141 gcctggtggc tatgaccgtc gcagcaagtg ttcaggctaa aactctggtt tattgctcag
    13201 aaggatctcc ggaagggttt aacccgcagc tgtttacctc cggcaccacc tatgacgcct
    13261 cttccgtacc gctttataac cgtctggttg aatttaaaat cggcaccact gaagtgatcc
    13321 cgggcctcgc tgaaaagtgg gaagtcagcg aagacggtaa aacctatacc ttccatctgc
    13381 gtaaaggtgt gaagtggcac gacaataaag aattcaaacc gacgcgcgaa ctgaatgccg
    13441 atgacgtagt gttctcgttc gatcgtcaga aaaacgcgca aaacccgtac cataaagtct
    13501 ctggcggcag ctacgaatac ttcgaaggca tgggcttacc agagctgatc agcgaagtga
    13561 aaaaggtgga cgataacacc gttcagtttg tgctgactcg cccggaagcg ccgttcctcg
    13621 ctgacctggc aatggacttc gcctctattc tgtcaaaaga atatgctgac gcgatgatga
    13681 aagccggtac accagaaaaa ctggacctca acccaatcgg taccggcccg ttccagttac
    13741 agcagtacca gaaagattcc cgtattcgct ataaagcgtt tgatggctac tggggcacca
    13801 aaccgaagat cgatacgctg gtcttctcta ttactcctga cgcttccgtg cgttatgcga
    13861 aattgcagaa gaacgaatgc caggtgatgc cgtacccgaa cccggcagat atcgcccgca
    13921 tgaagcagga taaatccatc aacctgatgg aaatgccggg gctgaacgtc ggttatctct
    13981 cgtataacgt gcagaaaaaa ccactcgatg acgtgaaagt tcgccaggct ctgacctacg
    14041 cggtgaacaa agacgcgatc atcaaagcgg tttatcaggg cgcgggcgta tcagcgaaaa
    14101 acctgatccc gccaaccatg tggggctata acgacgacgt tcaggactac acctacgatc
    14161 ctgaaaaagc gaaagccttg ctgaaagaag cgggtctgga aaaaggtttc tccatcgacc
    14221 tgtgggcgat gccggtacaa cgtccgtata acccgaacgc tcgccgcatg gcggagatga
    14281 ttcaggcaga ctgggcgaaa gtcggcgtgc aggccaaaat tgtcacctac gaatggggtg
    14341 agtacctcaa gcgtgcgaaa gatggcgagc accagacggt aatgatgggc tggactggcg
    14401 ataatgggga tccggataac ttcttcgcca ccctgttcag ctgcgccgcc tctgaacaag
    14461 gctccaacta ctcaaaatgg tgctacaaac cgtttgaaga tctgattcaa ccggcgcgtg
    14521 ctaccgacga ccataacaaa cgcgttgaac tgtacaagca agcgcaggtg gtgatgcacg
    14581 atcaggctcc ggcactgatc atcgctcact ccaccgtgtt tgaaccggta cgcaaagaag
    14641 ttaaaggcta tgtggttgat ccattaggca aacatcactt cgaaaacgtc tctatcgaat
    14701 aattaaaagc catacaagac tgatggcaaa ggcaaaactg cctgatgcgc tccgcttatc
    14761 aggcctacga aaactctgca atgtattgag tttgcacgat tttgtaggtc ggataaggcg
    14821 tttgcgccgc atccggcata aacaaagcgc tctttgtcaa caatctgcat acccggtggc
    14881 gctgtgcctc tgcgtgcagc gccatccggc agcaatactt cattccctgt tccacaaggg
    14941 cgggaattga tttgtgagca atacagacac gcagttccag gctgcgggtc attatagaga
    15001 atccgggtta tgttgcagtt tattctccga cgtttgggac tcgtcatccc cacgtttatc
    15061 ggtattaccc ttctcacatt tgcctttgtc cacatgatcc cgggcgatcc ggtgatgatc
    15121 atggcgggcg agcgtgggat ctccccagag cgtcacgcgc agctgctggc tgaactcggc
    15181 ttagataaac cgatgtggca gcagtatctc cattacattt ggggcgtaat gcacggcgat
    15241 ttaggcattt cgatgaaaag ccgcatcccg gtatgggaag agttcgtgcc gcgcttccag
    15301 gccacgctgg aacttggcgt ctgcgcgatg atttttgcta cggcagtcgg tattccggtt
    15361 ggtgtgctgg ctgcggttaa acgcggttcc attttcgatc acacggcggt tggcctggcg
    15421 ctgacaggtt attcaatgcc tatcttctgg tggggcatga tgctgatcat gctggtttcg
    15481 gtgcactgga acctgacgcc cgtctccggt cgcgtgagcg acatggtgtt cctcgatgat
    15541 tccaatccgt taaccggttt tatgctgatc gacaccgcca tctggggtga agacggtaac
    15601 tttatcgatg ccgtcgccca tatgatcttg cccgccattg tgctgggtac tatcccgctg
    15661 gcggtcattg tgcgtatgac acgctcctcg atgctggaag tgctgggtga agattacatc
    15721 cgcaccgcgc gcgccaaagg gttgacccgc cttagggtga ttatcgtcca tgcgctgcgt
    15781 aacgcgatgc tgccggtggt gaccgttatc ggcctgcagg tgggaacgtt gctggcgggg
    15841 gcgattctga ccgaaaccat cttctcgtgg cccggtctgg ggcgctggtt gattgacgca
    15901 ctgcaacgcc gcgactatcc ggtagtgcag ggcggcgtat tgctggtggc gacgatgatt
    15961 atcctcgtca acttgctggt cgatctgctg tacggcgtgg tgaacccgcg tattcgtcat
    16021 aagaagtaag gggacatcat gtcacaggtt actgaaaata aagtgattag cgcaccggtg
    16081 ccgatgaccc cgttacagga gttctggcac tattttaaac gcaacaaagg cgcggtcgtc
    16141 gggctggttt acgtcgtcat cgtgctgttt atcgcgatct ttgccaactg gattgcaccc
    16201 tataacccgg cggaacagtt ccgcgatgca ctgctcgccc cgccagcctg gcaggaaggc
    16261 ggcagcatgg cgcacttgct gggcaccgat gacgtaggcc gtgatgtgct gtcgcgtctg
    16321 atgtacggtg cgcgattgtc actgctggtt ggctgtctgg tagttgtgtt atcgctgatt
    16381 atgggcgtta ttctcggcct gatcgccggt tacttcggcg gcctggtcga taacatcatc
    16441 atgcgcgtgg tcgatatcat gctggcgctg ccaagtctgc tgttggcact ggtgctggtg
    16501 gcgattttcg gcccgtcgat tggtaacgcc gcgctggcgc tgacctttgt tgccctgccg
    16561 cactatgtgc gcttaacccg cgccgccgtg ctggtggaag ttaaccgcga ttacgttacc
    16621 gcgtctcgcg tggcaggtgc cggggcgatg cgtcagatgt ttattaacat cttcccgaac
    16681 tgccttgcgc ctctgattgt tcaggcgtcg ctcggtttct ctaacgccat tctcgatatg
    16741 gctgctcttg gtttcctcgg catgggggcg cagccgccaa cgccggagtg gggcaccatg
    16801 ctctccgacg tgttgcagtt cgcgcaaagc gcctggtggg tcgtgacctt cccgggtctg
    16861 gcgatcctgc tgacggtgct ggcatttaac ctgatgggtg acggtctgcg tgacgcgctc
    16921 gatcccaaac tgaagcagta agaggttcga gatggcgtta ttaaatgtag ataaattatc
    16981 ggtgcatttc ggcgacgaaa gcgcaccgtt ccgcgccgta gaccgcatca gctacagcgt
    17041 aaaacagggc gaagtggtcg ggattgtggg tgagtccggc tctggtaaat cggtcagttc
    17101 gctggcgatt atggggctga ttgattatcc gggccgcgta atggcggaaa agctggagtt
    17161 taacggtcag gatttgcagc gtatctcgga aaaagagcgc cgcaaccttg tgggtgccga
    17221 agtggcgatg atcttccagg acccgatgac cagccttaac ccgtgctaca ccgtgggttt
    17281 ccagattatg gaagcgatta aggtgcatca gggcggtaat aagagcactc gtcgtcagcg
    17341 ggcgattgac ttgttgaatc aggtcggtat tcccgatccg gcttcgcgtc tggatgttta
    17401 cccgcatcag ctttccggcg gcatgagcca gcgcgtgatg attgccatgg cgattgcctg
    17461 tcggccaaaa ctgctgattg ccgatgaacc aaccactgcg ctggacgtga ccattcaggc
    17521 gcaaatcatt gaactactgc tggagctaca gcagaaagag aacatggcgc tggtgttaat
    17581 tacccatgac ctggcgctgg tggcggaagc ggcacataaa atcatcgtga tgtatgccgg
    17641 tcaggtggtg gaaacgggcg atgcgcacgc catcttccat gcgccgcgtc acccgtatac
    17701 tcaggcattg ctgcgtgcgc tgccggaatt tgctcaggat aaagaacgtc tggcgtcgtt
    17761 gccaggtgtt gttcccggca agtatgaccg cccgaacggc tgcctgctta acccgcgctg
    17821 cccctatgcc actgacagat gtcgcgctga agaaccggcg ctgaatatgc ttgctgacgg
    17881 gcgtcagtcc aaatgccatt acccacttga tgatgccggg aggccgacac tatgagtacg
    17941 caagaggcca cctcgcaaca accgctgttg caggctatcg acctgaaaaa acattatccg
    18001 gtgaagaaag gtatgttcgc gccggaacgt ttggttaaag cgctggacgg cgtttcgttt
    18061 aaccttgaac gcggcaaaac gctggcggta gtaggggaat ctggctgcgg taaatcgacc
    18121 ctcggtcggt tgctgacgat gatcgaaacg cccaccggtg gtgaactgta ttaccaaggg
    18181 caggatctgc tcaagcacga tccgcaggcg cagaagctgc gtcggcagaa aatccagatt
    18241 gtcttccaga acccttacgg ttcgctgaat ccacgtaaaa aagtcgggca aattcttgag
    18301 gagccgctgc tgattaatac cagcttaagc aaagaacagc gtcgggaaaa agccctgtcg
    18361 atgatggcga aagtcggcct gaaaaccgaa cattatgacc gctatccgca tatgttctcc
    18421 ggcggtcagc gtcagcgtat cgccattgcc cgaggtctga tgctcgaccc ggatgtggtg
    18481 attgccgatg agccggtttc cgcgctggac gtctcagtgc gcgcgcaggt gctgaatctg
    18541 atgatggatt tgcagcagga gttggggctg tcttatgtct ttatctcgca cgacctgtcg
    18601 gtggtagagc acattgctga tgaagtgatg gtgatgtacc tgggccgctg cgtggagaag
    18661 ggaacgaagg accaaatctt caataacccg cgtcatccgt acactcaggc gctactctcc
    18721 gcgacgccgc gcctgaaccc ggacgatcgc cgcgagcgca tcaagctcac cggtgaactg
    18781 ccaagcccac tgaatccacc gccgggctgc gcgtttaacg cccgctgtcg tcggcgcttc
    18841 ggcccctgca cccagttgca gccgcagcta aaagactacg gcggtcaact ggtagcttgt
    18901 tttgctgttg atcaggatga aaatccgcag cgttaactca caaaaccggg gccgtgcgct
    18961 ccggtttact caatcagctt caacagtggc gacaagcata gcaaaatgcc gtaaagcaga
    19021 atcagccagg ctttaaaccc gcgaagtttt tccagttgtg cgactttata gatcaggaaa
    19081 aatggaatga ggcacgacac aatgccatat aacgggcttc ccaactgaaa gaacaccagc
    19141 accgatacac gaaacgagac ccaaatcgtc aacgtaatga cgataaaagc gcagatcgcc
    19201 agagtcagca tgcgtgagtt aattttcttg gtatcaataa ttcggcttaa cagattgaga
    19261 ataatgcctt taatggcctc gtggaaaccg agataaatgc caaagaatgc ggtcagtacg
    19321 gcaaagatat tcagcaccgt cgaggtgata tgaatgatat gcccagggat cacctgcgcg
    19381 gccagtgcca gtgccgagat attttgttca aaggcagaaa cggcttcttc gtggctaatt
    19441 gagaaggtaa acgaaaaggc aaaaaatagg atcaccgcga tgagcgtgac ataactaatt
    19501 cggtgggtac gcagcgcgag ccgggttgcc agtactttat ccgcttcccg tttgcggtag
    19561 gcaatattca tcgggtttag cacctgaata aataccgcag aaaagaagca aaaaggaata
    19621 gtaagcagga cgtcgcggaa gaagacggag gcttgcggga aggcggttat attggcgaaa
    19681 ttccagtgcg ggatcatcgc aaaaccgaag acgacaataa tccctacttt gaccaccacc
    19741 attggcccgg aaatcttaaa cagtaatcgt tcaccaccag acgcaattgc caccagtacg
    19801 gcgaaaatgg cgactttata aagtagagat tgtgaaagat cggcatcggt taaaccgaag
    19861 gttttcaggt acgaggcgct gtcgaaaacc acggagagag agtagataaa aatcccgtgg
    19921 ataatcatca aaaagtagat aaccccgagg aaaattcccc agttcttccc caggtaatga
    19981 ctgataatat cggtgtagtc attacaggaa tcgctttcag aaagggtttt taaataaatg
    20041 tcctgcacta cccaggtggc aggataagca atgatcgctg cggtaataaa tacccaaatt
    20101 cccttcaagc caatttgtac tggcatcagc acggttccgg caccaatcgc catgcctatg
    20161 cataataaaa cccagccgaa gtcgtagcgt gtaaacggca ttttctgatc gtgtttcggt
    20221 agtgtgttgt gctgcataaa gtcctgtcca gaaagaagtc ggcctcgcaa aaatgaataa
    20281 acccagtttc tcttgcgcaa caggtggaga agtcgaatac gcagtatgta aaaaatgagg
    20341 caacagattc actggggagg tcgcgaggag gcgcaattct acaaaattgc gcatttcact
    20401 accagtctgg agtcagcccg gatatgaact gcaactaatg caaataaaga aattaagctg
    20461 gaaggcatca cgcaatgagg aagattgccg ggtgctgacg gtaaaaagca atcgtaaaat
    20521 cagcggcaag ccggaaaggt tccggtgagg cgcaatgttg cgggggctta atccctggtg
    20581 gcattggttg ctggagagag aaaacccccg cacgttgcag gtatgcacct gacaacatca
    20641 cgggggctaa tcttgaacct agaccactca agaatagccg cgaaacgttg tcattacaac
    20701 acaggcggct atatgacgct cgcagaactg ggcatggcct tctggcatga tttagcggct
    20761 ccggtcatag ctggcattct tgccagtctg atcgtgaact ggctgaacaa gcggaagtaa
    20821 cgtgtcagac gggcgtcagg ctgccgtaat ggcaatttgc gcccggatca ggccgcaggg
    20881 ataaaactct gcggcctttt tcgtaagaaa aatgcaacgg ttgcaagcgg ataatattct
    20941 gcggaatgca cagcacagag tgaagaagtg acggatttcg gagctgaaaa acagtcgtag
    21001 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc ttaatccctg
    21061 gtggcattgg ttgctggaga gagaaaaccc ccgcatattg caggtatgca cctgacaaca
    21121 tcacgggggc cattcttgaa cctacacaac tcaagaatag ccgcgaaacg ttgtcattac
    21181 aactcaggcg gctatatgac gctcgcagag ctgggcatgg ccttctggca tgatttagcg
    21241 gctccggtca ttgctggcat tcttgccagc atgatcgtga actggctgaa taagcggaag
    21301 taacgtgtca gacgggcgtc aggctgccgt aatggcaatt tgcgcccgga ccaggccgca
    21361 gggataaaac tctgcggcct ttttcgtaag aaaaatgcaa cggttgcaag cggataatat
    21421 tctgcggaat gcgcagcaca gagtgaagaa gtgatggatt ccggaacaga aaaacagtcg
    21481 tagaatcagc ggcaagccgg aaaggttccg gtgaggcgca atgttgcggg ggcttaatcc
    21541 ctggtggcat tggttgctgg agagagaaaa cccccgcacg ttgcaggtat gcacctgaca
    21601 acaccacggg ggccattctt gcatctagac cattcaagaa tagccgcgaa acgttgtcat
    21661 tacaactcag gcggctatat gacgctcgca gagctgggca tggccttctg gcatgattta
    21721 gcggctccgg tcattgctgg cattcttgcc agcatgatcg tgaactggct gaataagcgg
    21781 aagtaacgtg tcagacgggc gtcaggctgc cgtaatggca agttgcgccc ggatcaggcc
    21841 gcagggataa aactctgcgg cctttttcgt aagaaaaatg caacggttgc aagcggataa
    21901 tattctgcgg aatgcgcagc acagagtgaa gacgtgacgg attccggagc agaaaaacag
    21961 tcgtagaatc agcggcaagc cggaaaggtt ccggtgaggc gcaatgttgc gggggcttaa
    22021 tccctggtgg cattgtttgc tggagagaga aaacccccgc acgttgcagg tatgcacctg
    22081 acaacaccac gggggccatt cttgaaccca aacaactcaa gaatagccgc gaaacgttgt
    22141 cattacaact caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat
    22201 ttagcggctc cggtcattgc tggcattctt gccagcatga tcgtgaactg gctgaacaag
    22261 cggaagtaac gtgtcatgcg ggcgtcaggc tgccgtaatg gcaatttgcg cccggatcag
    22321 gccgcagggg tgaaactctg cggccttttt cgttcttatt gcgggtaagg cacccagtcg
    22381 ccgccgttca ggcgaacgta cggtttatcc tggtattgaa taactactgc atttgagttc
    22441 tcggagaccg gtgctgtttg tggcaaccca ctggtgagtt ttttccagtc aacattgtct
    22501 tcggagaaaa tcttgccatc aagaacgcga accaccagat cggagatagc caggaagctg
    22561 ctcggttggt cgatgacaat cggtgccccc tgatgtggtg ccttcatgcc gaagaatttc
    22621 accccaacgg ggacgtctgt gatagacggg ctagggatat cacgtaggcc agatacctgc
    22681 attctgtcgc ccttcagcgc gccgccgtgt tccggcacca cgaccaccat cactttacga
    22741 cccgatttct ccagttcagt aaagaaggcg tccagttcat caaagaattt ctgcgcccgc
    22801 gctttgtaat ccgctgtttt gctgacgccc ggataatggt tgccgtcatg cagtggaagc
    22861 gtgttgtaga acgtggcact acggctgttt ttatcttttt cggtaatgtc cagccagcgg
    22921 ttaagcacgg cggtatcgtc ataaaccggc gaaccatcaa agcccagcaa aataaccggc
    22981 agatttgttt gatccatcaa ttcggtctgc atgccgccat tttcgcgaac ttctttcaaa
    23041 aaaccgccga actggccgtt atgtcccatc atcaggtgct gggtaaagcc cagtttcgac
    23101 aggttatcaa acagatagca gtcgttattt gccggttgat acagattagt gtgcgaagtc
    23161 tgcccgcagc tggcgcgcag taaacggatc gccgccgggc cactgtagga ggtggcggag
    23221 ttaaagttct tgaactcaat atcgaaatgc gaccacagcg gatgcgacat caacccggcg
    23281 gcttcaatat ccgaccagga aagcgaacag atattaatca ccagtagttc aaatggctga
    23341 gcatcagcgg gcagcgaaga cgggaaggtc gatttacgtt tcgcctccgc gttatagaaa
    23401 ttatttagcc aggcgttaag gttcgccgtt gttggcggtg cagtttgtgc gggcatatca
    23461 cccactaccg gtgcgccacc cgtcgccgca acggttgccg ctgcgttacc acccgtcgtt
    23521 gttacagtgg tcgtcggttg accggctggc cacaaggaga aacttggtcc cgccagggta
    23581 agtacgttca gccatagcag aatggcaacc acaaaaacgg taatgcgaat ccattgtgac
    23641 aggaataacc aggccacgaa taaaacaaaa atggccccaa tcatctgcca gttaataaag
    23701 cgtgtgacaa ggtcgattaa ataatcggta ctgaaccccg ccacctgcga accctggctc
    23761 attatgcttt ccgggccagg caaccaggtg tcatgccaga acaaagcaaa gccgatcggc
    23821 agggcaatcc agtggcgcaa gcgatgcagg ctgtagcgcg gaatgggcat cagcagaaac
    23881 gcggcaaaga ccaaattgag gagcggatgg aagttaagat atcccgccca caacaggccg
    23941 aacttaacca gaaaatagaa gttccagccg gaaaggccgc gccagtattg ccagagggaa
    24001 gaaggcatgg cggtattttg cgtaaattga gtcatttttt ggttgccctg gctttttccg
    24061 tgcggcgtaa cgtcccggcc ggtttaacat aacgaggttt agcaaagaac aaacgtaagg
    24121 tgtcgcgaat gcgtcgcaaa gagtgccgcg ccagatagcc cagcgggaaa aatatcagtg
    24181 cgcaaacgac aataatttca atgatatcgc tgatggtcat catgatgagc gctccacagc
    24241 atcatctaac agtcgcattg gttctggtat tcgtcgccag tggcgaccat cgtgctcggc
    24301 attgatgacc ggtttagaac tttgcgttaa aggcagcggc atgccccatt gttctggggc
    24361 aagcaggcgc atctgcacca gctcggcact gatttgatca tcttcaaacc agaccatacg
    24421 gtttgagaaa atgtcgccag taggcaatgg gaaaatatga ttcaacgcgg tatccagatc
    24481 gttaatccga cagaatgaga gaaacagcac cagccgatta ccgccaatgg tcatgatatc
    24541 gccggtgcga ttagggcgac atagcgtcag ggcttgctca acgcggatac ccggtaccgg
    24601 acgtagggca accagaacgc ctttaccgtg ggcaggtaat agagggttat tcatcatgtt
    24661 gttgacggca ttacagaaca catcccactt ctggaaacca cgcagtttga gcggctgggt
    24721 cattgacagc aaggtagtga tatcttccgg cacatagcga ctaaacttct gcccttgcac
    24781 gctttcgatc atcgtcagac agcgggagag tggcgcattc cacgggataa ccatatttgc
    24841 accgcaggcc aataataaac gttcatcggt ggcgcgcagg ctagcggtat tttcccgtac
    24901 gaggattttc atcgcactac cgcgctggcg acgcagagta tgaatgctgc gggccagtgg
    24961 ctcgatttgc gcattttgct gtaaagaaaa gaccaccgtc gccgcctgag cggtacgggc
    25021 ttcattgaac aggacttcgt tattgttgaa cagttgccag tgttccgata gcggcggcgc
    25081 accttccagt acagcaacat tactcagaat gcgtttttcg tcgctgcgtg gttggatttc
    25141 cgcctcctgg tgttgaacta atgtccagca accattttgt tgctgaacgc taagctgctg
    25201 acgggcgctg accccttttt cgttgcacca gaaagcaata tccagcaaat gttggtcgcc
    25261 ctgaaaacgc aaactggcaa gaccaaaaag tgaacggtat tcctcaagta acaatgaaaa
    25321 ttgtttatcg ttattatttc cggaattaat taccaaaagc gaacaatggt ttagccggct
    25381 ccatttattc attttatcca accatgagcg aagccgctcg gcaggaatgt tttgccatgc
    25441 gttatttgcg caaacaagaa taaaaaggta attatgggga tcaatagaac actgcaaatc
    25501 acgggccaaa tagtatagac ctttttcatg atttagcatt gaaaataatg ttatttttcc
    25561 cggcccttga gaatcatcta attgaaagat tttcgctgga tcgctgtcca tgctaatgac
    25621 cgcgacgtgt gcggtttcag cctgggatgc aattgtttga ttcgccagac tgatagcatc
    25681 ttcatggcga tcgacgttaa accaccagac gccgcctgct ggcatatgtc gcagctcatc
    25741 ccataatgat gagataccga tagagaatac agggtccaca atgtccctca tgtcaacatt
    25801 ttgtctgatt atcagtttac tagcgaaagc taagaaataa acctaccatt gaaattaaaa
    25861 cttatcagat ttagcatgtg aatcaattta gcaggtcaca agacatgtga ttttagtctt
    25921 ttcgttttta actttttctg ttgcgtaacg gaaagtcaaa aagtgagcac attcccgtct
    25981 cgccgtaaac aataaaccga cgaaatgctc acagttagaa ttgtctcatg aacggtacgg
    26041 ttatttcata gggatcaaga aaaatgaata acaatgaacc agatactctg cctgatcccg
    26101 cgataggcta tatcttccag aatgatattt tggcgttaaa gcaggcattt tcactgcctg
    26161 atattgatta tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc
    26221 cgttgctggc agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt
    26281 gcagggggtt cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt
    26341 acaaatgttg ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct
    26401 gtcatttaac gttgatttta cccaccgtca gggttgggcc agagctatgc tggatggcca
    26461 ggactggcgt gacgccgggt tgcgctatac ctcgcagctc gatttgctgc cttttggtca
    26521 gttatcgatt gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg
    26581 caccggctta cagcaactaa aagccagcgg gcgttaccag tggattttaa ttgacttacc
    26641 gcgtgatgcc tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat
    26701 cgtcaatgtg gatgccaact gccatatccg actgcatcag caagcgctgc cggatggtgc
    26761 acatattttg attaatgact tccgtattgg cagccaggtt caggacgata tttaccagct
    26821 ttggttgcaa agccagcgcc gattgctgcc gatgctcatt catcgtgatg aagcgatggc
    26881 tgaatgtctg gcagctaagc aaccagtcgg tgaatatcgc agtgatgcgc tggcggctga
    26941 agagatactg acgctggcga actggtgcct gttgaattat tccgggctga aaacgccagt
    27001 cgggagtgca tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcacgg
    27061 cttattgggc gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc
    27121 ggctgtttct ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctggcag
    27181 cgtattcgcg cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg
    27241 ctggacccgg tccgttatct tattcaaaca tgctggttac tgatcggtgc atcgcgcaaa
    27301 gaaacgccga aaccgcgcag gcgggcattt tcaggtctac aaaatattcg tggacgttac
    27361 catcaatgga tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag
    27421 aaaaaagagc tcggtcattt gagtgccggg gcgcggcggt tgatccttgg tatcatcgtc
    27481 accttctcgc tgattctggc gttaatctgc gttactcagc cgtttaaccc gctagcgcag
    27541 tttatcttcc tgatgctgct gtggggggta gcgctgatcg tacggcggat gccggggcgc
    27601 ttctcggcgc tgatgctgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg
    27661 cgttacacct cgacgttaaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg
    27721 ctgttcgccg aaacgtacgc gtggatagtg ctggtgctcg gctacttcca ggtcgtatgg
    27781 ccgctgaatc gtcagccggt gccattgccg aaagatatgt cgctgtggcc gtcggtggat
    27841 atctttgtcc cgacttacaa cgaagatctc aacgtggtga aaaataccat ttacgcttcg
    27901 ctgggtatcg actggccgaa agacaagctg aacatctgga tcctcgatga cggcggcagg
    27961 gaagagtttc gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg taccactcat
    28021 gaacatgcga aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg
    28081 tcgatattcg actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg
    28141 ttcctgaaag agaaacagct ggcgatgatg cagacgccgc accacttctt ctcgccggat
    28201 ccgtttgaac gcaacctggg gcgttttcgt aaaacgccga acgaaggcac gctgttctat
    28261 ggtctggtgc aggacggcaa tgatatgtgg gatgccactt tcttctgcgg ttcatgtgcg
    28321 gtgattcgcc gtaagccgct ggatgagatt ggcggtattg ctgtcgaaac ggtaaccgaa
    28381 gatgcgcaca cttctctgcg tctgcaccgt cgtggctaca cctcagcgta tatgcgtatt
    28441 ccgcaggcag cggggctggc gaccgaaagt ctctcggcgc atatcggtca gcgtattcgc
    28501 tgggcgcgcg ggatggtgca aatcttccgt ctcgataacc cactcaccgg taaagggctg
    28561 aagtttgctc agcggctgtg ctatgtcaac gccatgttcc atttcttgtc gggcattcca
    28621 cggctgatct tcctgactgc gccgctggcg ttcctgctgt ttcatgccta catcatctac
    28681 gcgccagcgt tgatgatcgc cctgttcgtg ctaccgcata tgatccatgc cagcctgact
    28741 aactcgaaga tccagggcaa atatcgccac tctttctgga gtgaaatcta cgaaacagtg
    28801 ctggcgtggt atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa
    28861 tttaacgtca ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg
    28921 cggccctaca tcttccttgt tctgctcaac ctggtgggcg tagcggttgg gatctggcgc
    28981 tacttctatg gtccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtattc
    29041 tacaacctga ttattctcgg cggcgcagtc gcagtatcgg tagaaagcaa acaggtacgc
    29101 cgatcgcacc gcgtggagat gacgatgccc gcagcaattg cccgcgaaga tggtcacctc
    29161 ttctcgtgta ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag
    29221 gcgcagattc tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac
    29281 gtcttcccgg ctcaggtggc gcgtgtgatg ggtaatgaag ttgggctgaa attaatgccg
    29341 ctcactaccc agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg
    29401 gcgctctggc aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg
    29461 aagctcggct tccgtggcta ccgccatctg gcggaatttg cgccttcttc ggtgaagggc
    29521 atattccgtg tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccgcccg
    29581 gagcggagcg aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc
    29641 gatgaaaaga aaaatattct ggatttgtgc agtggctctg gggatgagtg cgttcccctc
    29701 tttcatgacg caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc
    29761 cgcccaggcg gagcaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgcaga
    29821 tgcgccagtc gtggcgcaga acggtccttc ccgtgatgtg aagctgacct ttgcgcaaat
    29881 tgcgccgccg ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt
    29941 tggtatgcgc agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc
    30001 gccatcatta ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg aactgatggg
    30061 cgtgctgcca gtgaccaaag aacagttggg taaaaaaacg ctggcacaaa tgccaattaa
    30121 cccactgttt attaccgact tcaaccgtgt gcggctggag tttgtcggtc attatcagga
    30181 cgtgtgcgaa aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact
    30241 ggatctgacc tatcagaccc tgaatgtgaa gaatgacctg tcacacttcc cggtgccatt
    30301 ctttgacccg cgtgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga
    30361 tgtcgggctg caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg
    30421 gcgtgggcag aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccatcgtttt
    30481 tgccaccaat gacaaacgcc cggacttcct gcgcgatcat ccggcggtaa aagccccggt
    30541 gattgaaatg atcaaccatc cgcagaatcc ttatgtcaaa ctgctggtgg tgtttggtcg
    30601 tgacgacaaa gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg
    30661 tggtgaaagc gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc
    30721 gccgaactgg gtgcgtaccg atcgtccggt tacctttggt gaactgaaaa cctatgaaga
    30781 acagttacaa tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccacc
    30841 ggatctctac ctgatgcgca gtaccggcat tgatatggat attaattatc gctacaccat
    30901 gccgccagtg aaagacagct cacggatgga tatcagcctg aataaccagt tcctgcaatc
    30961 cttcaacctg agcagtaaac aggaggcgaa ccgcctgctg ctgcgtattc cggtattaca
    31021 aggtttgctg gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa
    31081 ccagctgcgc tttgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg
    31141 cattaccttc cagccggtgc agaatcatgt ggtgattggt gatgattcca ccatcgactt
    31201 ctcgaagtat taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt
    31261 cccgttcagc cggatggcgg atctgtcaca aaccatcacc gtgatgccga aagcgcctaa
    31321 cgaagcacag atggaaacct tgctgaatac cgttggcttt atcggtgcgc agacgggctt
    31381 cccggcgatt aacctgacgg tgaccgatga tggcagcact attcagggga aagatgccga
    31441 catcatgatc gtcggtggta tcccggacaa actgaaagac gacaagcaga tcgacctgtt
    31501 ggtgcaggcg accgaaagct gggtgaaaac gccgatgcgc cagaccccgt tccccggcat
    31561 tgtaccggac gagagcgatc gcgcggcaga aactcagtca acgctgacct cttcaggtgc
    31621 gatggcagcg gtgattggct tccagtcgcc gtataacgac cagcgtagcg tgattgcgct
    31681 gctggcagat agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa
    31741 acgcgccacc atgttcggtt cggtcgcggt gatccgcgag tccggtatca atagcctgcg
    31801 tgttggcgac gtctattacg ttggccatct gccgtggttc gaacgcttat ggtatgcgct
    31861 ggcaaaccat ccgattctgc tggcggtgct ggcggcaatc agcgtcgtgc tgctggcatg
    31921 ggtactgtgg cgtctgctgc gtattatcag ccgtcgtcgt cttaacccgg ataacgagta
    31981 attgaagatg aatgtgttgc gtagtggact cgtgacgatg ctgctgctgg ctgcctttag
    32041 tgttcaggca gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca
    32101 ggaagggcgc gtcatcgacc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag
    32161 ttacggcatg ttctttgccc tggcggctaa cgaccgtgca gctttcgata atcttctcga
    32221 ctggacgcag aacaatctcg cacagggttc tttaaaagaa catttgcccg cctggctgtg
    32281 gggcaagaaa gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga
    32341 tgtctggatg gcctgggcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac
    32401 cgacatcggc agcgcattgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg
    32461 gctgggctcc atgttgttac cgggcaaagt gggatttgca gaggataaca gctggcgttt
    32521 taaccccagc tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg
    32581 gaccacgctg cgcgaaacca atcaacgttt attgctggaa accgccccga aaggcttttc
    32641 gccagactgg gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt
    32701 gatcagcagc tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga
    32761 tccgcaaaaa gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa
    32821 cggttatccg ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccggt
    32881 cggtttttct gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg
    32941 ccagcgcgtg gccgataact ttcctggcag cgatgcctat tacaactatg tgctgaccct
    33001 gtttggacaa ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc
    33061 tgactggggc caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg
    33121 gccgtcatgc cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt
    33181 cggttaggcg aagcgaccca tcgtgaagac ctggtgcaac agtcgttgta tcgtctggaa
    33241 cttattgatc cgaataaccc ggacgtcgtt gctgcccgtt ttcgttctct gttacgtcag
    33301 ggcgatattg atggcgcaca aaaacagctc gatcggctgt cgcagttagc gccgagttca
    33361 aatgcgtata aatcgtcacg gactacgatg ctgctttcca cgtcggatgg tcgtcaggca
    33421 ctgcaacagg caagattgca ggcgacgact ggtcatgcgg aagaagctgt ggcgagttac
    33481 aacaaactgt tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg
    33541 gtggcgaaaa ttccggcccg acgtggcgaa gcgattaatc agctaaaacg cattaatgcg
    33601 gatacgccgg gcaacacggg cctgcaaaac aatctggcgc tattactgtt tagtagcgat
    33661 cgccgtgatg aaggttttgc cgtcctggaa cagatggcga aatcgaacgc cgggcgcgaa
    33721 ggggcctcta aaatctggta cgggcagatt aaagacatgc ctgtcagcga tgccagcgtt
    33781 caggcactga aaaaatatct ctcgatcttt agcgatggcg atagcgtagc tgctgcacaa
    33841 tcgcaactgg cagaacagca aaaacagctg gccgaccctg ctttccgcgc tcgtgcgcaa
    33901 ggtttagcgg cggtggactc tggcatggcg ggtaaagcca ttcccgaact acaacaggcg
    33961 gtgcgggcga acccgaaaga cagtgaggct ctgggggcgc tgggccaggc gtattctcag
    34021 aaaggcgatc gcgctaatgc ggtggcgaat ctggaaaaag ccctcgcgct ggatccgcac
    34081 aacagcaata acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc
    34141 cagcagggcg atgccgcgct gaaagccaat aatcctgacc gggcagaacg gctgttccag
    34201 caggcacgta atgtcgataa caccgacagc tatgcagtgc ttgggctggg cgatgtggcg
    34261 atggcgcgca aagattatcc cgccgccgaa cgctattacc agcagacctt gcgtatggac
    34321 agcggcaaca ctaacgccgt gcgcgggctg gcgaatattt accgccagca gtcgccagaa
    34381 aaagccgaag cgtatatcgc ctcgctctct gccagccagc ggcgcagcat tgatgatatc
    34441 gaacgcagcc tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggg
    34501 aaatgggcgc aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagtgta
    34561 tggattactt accgactttc acaggatctc tggcaggccg gacaacgcag ccaggccgga
    34621 caacgcagcc aggccgatac gttaatgcgt aatctggcgc agcagaagcc gaacgacccg
    34681 gagcaggttt acgcttacgg gctgtatctc tctggtcatg accaggacag agcggcgctg
    34741 gcgcatatca acagcctgcc gcgcgcgcag tggagcagca atattcagga gctggttaat
    34801 cgactgcaaa gcgatcaggt gttggaaacc gctaatcgcc tgcgagaaag cggcaaagaa
    34861 gctgaagcgg aagcgatgct gcgccagcaa ccaccttcca cgcgcataga cctcacgctg
    34921 gctgactggg cgcaacaacg acgtgattac accgccgctc gcgctgcata tcagaatgtc
    34981 ctgacgcggg agccaactaa cgccgatgcc attcttggtc tgacggaagt ggatattgct
    35041 gccggtgaca aagcggcggc acgtagccag ctggcgaagc tgcctgccac cgataacgcc
    35101 tcgctgaaca ctcagcggcg cgtggcgctg gcacaggcgc agcttggcga taccgcagcg
    35161 gcgcagcaga cgtttaataa gttgatcccg ctggcaaaat ctcagccgcc gtcgatggaa
    35221 agtgcgatgg tactgcgtga tggtgcgaag tttgaagcgc aggcgggcga tccaacgcag
    35281 gcgctggaaa cctacaaaga cgcgatggtt gcatccggtg tgaccacgac gcgtccgctg
    35341 gataacgaca cctttacccg actgacccgc aatgacgaga aagatgactg gctgaaacgc
    35401 ggcgtgcgca gcgatgcggc ggacctctat cgccagcagg atcttaacgt cacccttgag
    35461 catgattact ggggttcgag cggcaccggt ggttactccg atctgaaagc gcacactacc
    35521 atgttgcagg tggatgcgcc gtattctgac gggcggatgt tctttcgcag tgatttcgtc
    35581 aatatgaacg tcggcagttt ctccactaat gccgatggta aatgggatga caactggggc
    35641 acctgtacat tacaggactg tagcggcaac cgcagccagt cggattccgg tgccagcgtg
    35701 gcggtcggtt ggcgcaacga cacctggagc tgggatatcg gcaccacgcc gatgggcttc
    35761 aacgtggtgg atgtggtcgg cggcatcagt tacagcgatg atatcgggcc gctgggttac
    35821 accgttaacg cccaccgtcg gcccatctcc agttctttgc tggcctttgg tgggcaaaaa
    35881 gactccccga gcaataccgg gaaaaaatgg ggcggcgtgc gtgccgacgg cgtggggcta
    35941 agcctgagct acgataaagg tgaagcaaac ggcgtctggg catcgcttag tggcgaccag
    36001 ttaaccggta aaaatgtcga agataactgg cgtgtgcgct ggatgacggg ctattactat
    36061 aaggtcatta accagaacaa tcgccgcgtc acaatcggcc tgaacaacat gatctggcat
    36121 tacgacaaag acctgagtgg ctactcactc ggtcagggcg gttactacag cccgcaggaa
    36181 tacctgtcgt ttgccatacc ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag
    36241 ctgggggcgt ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg
    36301 atgaatctga tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc
    36361 agcagtcagg gcttcggcta tacggcgcgg gcattacttg aacgacgtgt tacttccaac
    36421 tggtttgtcg gcacggcaat tgatatccag caggcgaaag attacgcacc cagccatttc
    36481 ctgctctacg tacgttattc cgccgccgga tggcagggtg acatggattt accgccgcag
    36541 ccgctgatac cttacgccga ctggtaagtt ttcagatagc gcctctctta atgccgctgc
    36601 gatcgggtat actcgggcgg caatctggga tttccggggg gagacaattt gcgcgtaagt
    36661 cgctcgttaa caatcaagca gatggcaatg gtggcagccg ttgtcctggt gttcgttttt
    36721 attttttgca ccgttttgct gttccatctg gtccagcaga atcgctataa cacggctacg
    36781 caactggaaa gcattgctcg ctctgtccgc gaacccttat cttcagctat tttgaaaggc
    36841 gatattcccg aagcggaagc tattcttgcc agcattaaac cggcaggcgt ggtcagccgt
    36901 gccgatgtgg tgctgcccaa ccagttccag gcgctgcgta aaagttttat tccagagcgt
    36961 ccggtgccgg taatggttac tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtc
    37021 tactcgctcg aacgtccggc aaatccgcag ccaattgcct atctggtgct acaggcggat
    37081 tccttccgta tgtataagtt cgtgatgagc accctctcaa cgttagtgac catttactta
    37141 cttttgtcgc ttatcctgac cgtcgccatc agctggtgta ttaatcgact gattttacat
    37201 ccgttacgca atattgctcg cgaacttaac gccatcccag cccaggagct tgttggtcac
    37261 caactggcat taccacgtct gcatcaggac gatgaaatcg gtatgctggt gcgcagttac
    37321 aacctcaacc agcaattgct gcagcgccat tatgaagaac agaacgaaaa cgcgatgcgc
    37381 ttcccggtgt cggatttgcc gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc
    37441 gcgcgtaaac aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg tgatactgcg
    37501 ggcgtgctga aagaggcgca acgagaaatt ctgctgctga cgctggtgga aaaactcaaa
    37561 tcggtactgt cgccacgcat gatcctcgcg cagattagcg gttatgactt tgctgtcatt
    37621 gccaacggtg tacaggaacc gtggcacgct atcacattag gtcagcaagt gctcactatc
    37681 atgagcgagc gcctgccgat tgaacgtatt caactccgtc cgcactgtag cattggcgtg
    37741 gcgatgttct acggcgatct caccgccgaa cagctttaca gtcgcgctat ttctgcggca
    37801 tttaccgctc gccataaagg caaaaatcag attcagttct ttgatccgca gcagatggaa
    37861 gccgcccagc agcggttgac ggaagagagc gatatcctta atgcactgga aaatcatcaa
    37921 tttgcgattt ggttacagcc acaggtcgag atgaccagcg gtaaactggt cagtgcggaa
    37981 gtgttactgc gtatccagca accggatggc agttgggatc tgccggatgg cttaatcgat
    38041 cgcattgagt gctgtgggct gatggttacc gttggtcact gggtgctgga agagtcttgt
    38101 cgactgcttg ctgcctggca agagcgcggc attatgctgc ccttgtcggt aaacctctca
    38161 gcgctacaac tgatgcaccc gaatatggta gcggatatgc tggaactgtt aacccgctat
    38221 cgcattcagc cgggaacact gattctggaa gtgacagaaa gccgacgtat tgacgaccct
    38281 catgctgcgg tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat
    38341 gatttcggca tgggctacgc agggctgcgt cagctgcagc atatgaaatc gttgccaatc
    38401 gacgtactga aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag cagcatgatt
    38461 gctgcaatta tcatgttggc gcaaagcctg aacttacaaa tgattgccga aggcgtggag
    38521 actgaagcac aacgcgactg gctggcaaaa gcgggcgttg gtattgccca gggcttcctt
    38581 tttgctcgcc cactccctat tgaaatcttc gaagagagtt acctggaaga aaagtagcta
    38641 ccccaaactg attacaaaac tttaaaaagt gctggtttgt gcgagccagc tcaaactttt
    38701 taaccttttt gtttcaatta tgatccaggt acatttctgt gatgttgtct gggtgttatt
    38761 ttagggccgc aggtacccca taaccttaca agacctgtgg ttttactaaa ggacacccta
    38821 tgaaaacctc tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta
    38881 ttctccttgg ccatttctac cctgaaatag gcgagcaaat gaaaccgctt ggcgacgggt
    38941 tcgttaagct cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca
    39001 ttgcgggcat ggaaagcatg aaggcggttg gtcgtaccgg cgcagtcgca ctgctttact
    39061 ttgaaattgt cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc
    39121 ctggtgccgg aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg
    39181 ccgatcaggc gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg
    39241 tcattggcgc atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg
    39301 gttttgcgct ccaccgtctg ggcagcaaag gccaactgat ttttaacgtc atcgaaagtt
    39361 tctcgcaggt catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgt
    39421 tcggggcaat ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc
    39481 agctgattat ctgtttctac atcacctgta tcctgtttgt ggtgctggta ttgggttcaa
    39541 tcgctaaagc gactggtttc agtatcttca aatttatccg ctacatccgt gaagagctgc
    39601 tgattgtact gggcacttct tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg
    39661 agaaactcgg ctgccggaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta
    39721 accttgatgg cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggcgacta
    39781 acagtcagat ggatatcgtc caccaaatta cgctattaat tgtgttgttg ctctcttcca
    39841 aaggggctgc cggggtaacg ggcagtggct ttatcgtgct ggcggcgacg ctctctgcgg
    39901 tgggccattt gccggtagca ggtctggcgc tgatccttgg tatcgaccgc tttatgtcag
    39961 aagctcgtgc gctgactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt
    40021 gggtgaaaga actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg
    40081 gcaaaacgca cgaattatcc tcttaatctc atcacttatg cccgcactcc cttccccgag
    40141 tgcgggtgtt tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg
    40201 tttatttcac ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta
    40261 acgtcattta gactgaccga caacgggcag tttttttatt accaggatag ttgatcaggg
    40321 gttcacatgc agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact
    40381 gctggctatg tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt
    40441 tccaacggtt tacagtggca agtgctgacc acgccccagc gtcctagcga tcgtgttgaa
    40501 attcgcctgc tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagc
    40561 cacgccatcc ctcgtattgc gttaacgcaa agcggtggcc ttgaagcagc acaggcgcgt
    40621 tcattgtggc agcaggggat cgaccctaaa cgcccgatgc cgccagtaat tgtctcttat
    40681 gacaccacgc tgtttaatct gagtttgccc aataaccgta acgacttgct gaaagaagcg
    40741 ctctcttatc tggcaaatgc cactggcaaa ctgaccatca caccagaaac catcaaccac
    40801 gcgctgcaaa gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg
    40861 cgctatcgtc tgaaaggatc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa
    40921 cccgttgaag cggaaaagat taaagatttc tatcagaaat ggtatacccc ggatgcaatg
    40981 acgctgctgg tggtgggaaa cgtggatgcg cgctcggtcg tcgaccaaat caataaaacg
    41041 tttggcgaac tgaaaggcaa acgtgaaaca ccggctccgg tgccgacgct ttctccgctg
    41101 cgtgcggaag cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atcaatcatg
    41161 tgggatacgc cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg
    41221 gacctggccc gtgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagt
    41281 aaagacatcg gtcttggatt tgactgtcgt gtgctgtatc tgcgtgcgca gtgtgccatc
    41341 aacatcgaat caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg
    41401 gcgaaggttc gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag
    41461 aaactggagc tgcagaaact gtttgccgcc tatgcgcgag ctgataccga tattctgatg
    41521 gggcagcgga tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcaatatcag
    41581 aaactgcgtc aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt
    41641 cagcagttgt cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt
    41701 aacatgaaag cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgcggctgcc
    41761 gccacatctg tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgtacag
    41821 taatgcctga tggtgatgag ttagcacaac catcaggtat ttatggatga gctggatagg
    41881 ggggtaacac tctatccagc cttttgcata tgctgctttt acgctggcat cgcttcacgc
    41941 gggataatcg cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgct
    42001 gcgtcttccg cactgccgcc cgtcaaacgt accgccagat aaccggcact gaaagagtcg
    42061 ccagctgcgg tggtatcgat cactttttct ttcggcagtt ttaccgccgg aacatccact
    42121 aacccttcgc cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct
    42181 ttcacgcccg cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc
    42241 gcgtcttcat cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac
    42301 acctgctgtg tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc
    42361 acttttccgc cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc
    42421 tggcttaaga tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct
    42481 tcgcaaatcg ccgcggattg ctcactctcc agccagaatt tggcggcggc ttcgttacgc
    42541 cagtagtaga acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga
    42601 cggttttcca tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc
    42661 atctgctggc taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca
    42721 ggatcgacct gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc
    42781 ttaacgtccg cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt
    42841 ttggacatag tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt
    42901 tgctggtcaa ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg
    42961 aaagattccg ttgtggagtg gggaaattta ccccgaccag gcggtaaagt tctgcccttg
    43021 cgcgccgata atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga
    43081 tgataaggca ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg
    43141 aacggcgttt ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat
    43201 gcggacggtt aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc
    43261 aacgcctgcc gccggatcgc tattttactg aaatcaccgt tagccatcgg atggaggttg
    43321 tgaaagagca gattgatttg ctggcgcaaa aagccgactt ctttatagag cacgacctgc
    43381 tggcatcggt caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc
    43441 tgcgccaaat tgagcgtctt ccctggctgc gtttcgaact ggtggagcat atccgtctgc
    43501 cgaaagattc aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg
    43561 gcaccgggat ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg
    43621 cgcgagagct gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc
    43681 ttttacatct catgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacgccgg
    43741 aagagtggcg tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac
    43801 gcccggcacc gatagaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt
    43861 ccgcctggct ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga
    43921 gcaaggcagg caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg
    43981 cgatcatttt gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag
    44041 tctctgcgga gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc
    44101 ggcaaaaaca agaaactggc tggcgcagct gggtgccgtg gccccatgta cacgcggaag
    44161 acatcattct tggcaatcca ccggatattc ccgaagtcac aatggtgcat ttgccgcgag
    44221 tagaagcgac gctggccccg ctggcgctgc tgaccaaaac ggtctggttg ccgtggatca
    44281 agctcgaaaa gcccgacgca cgcctgattc gcctctcaga aaagaacaac aactggacat
    44341 ttaatctcgc cagcgatgac aacaaagaca cgaatgcaaa gccgtcggca tggtcgtttc
    44401 ggctggataa tattctgttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag
    44461 cggatctaga aatttttgtc gatccgttag gcaagccgct gccgttcagc gaagttactg
    44521 gatcgaaagg taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc
    44581 agggacgtta taacggagag ccgctcactg gtacgggaaa aataggcggt atgctggcgc
    44641 tgcgtggcga agggacgcca tttcctgtac aggctgattt tcgctctggt aacacccgtg
    44701 ttgcttttga tggcgttgtg aatgacccaa tgaagatggg tggtgtcgat ttacggctta
    44761 aattttctgg cgattcgctg ggtgatctct atgaactgac gggcgttctg ctgcccgata
    44821 ccccgccgtt tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg
    44881 tctttgatta tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg
    44941 tctacaccac cggcaagcca cgaccaaaac tggaaggtga tgttgagtcg cggcaattgc
    45001 ggctggcaga tttagggccg ttaattggcg ttgattccgg gaaaggagct gaaaagtcga
    45061 aacggtctga acagaagaag ggtgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt
    45121 atgaccgctt cgaaaccgat aaatgggacg tcatggatgc cgatgttcgc ttcaaagggc
    45181 ggcgcattga gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca
    45241 aaaatgctga cctgcgcctg caaccgctga aatttggcat ggcaggcggc agcattgcgg
    45301 cgaatattca tctggaaggt gataaaaaac cgatgcaagg gcgggcagat attcaggctc
    45361 gtcgactgaa actgaaagaa ctgatgcccg atgtagaact gatgcagaag acgctggggg
    45421 aaatgaacgg tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca
    45481 acagtaacgg caacctgaaa ctgctgatga atgacgggct ggtgagccgc aacctgatgg
    45541 agattgtcgg gctgaatgtc ggtaactaca ttgtcggtgc gatatttggt gatgatgagg
    45601 tgcgggtgaa ctgtgcggcg gcgaatctgg atattgccaa cggcgtggcg cgcccgcaga
    45661 tttttgcttt cgatactgag aacgcgttga ttaacgttac cggtacggca agttttgctt
    45721 cggaacagct ggatttgact attgatccgg agagtaaagg gattcggatt atcacattgc
    45781 gttcgccgct gtatgtacgt gggacgttta aaaatccgca ggctggggtg aaagccgggc
    45841 cgttgattgc tcgcggagcc gttgcggcgg cactggcaac gctggtaaca ccggcggcgg
    45901 cgttactggc gctgatctca ccttccgaag gggaggctaa tcagtgcagg acaattttgt
    45961 cgcagatgaa gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt
    46021 agattgttgg caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc tccggcttac
    46081 aaatccttgc taattcaata tattgtatgg aaattctggc ctgataagcg tagcgcatca
    46141 ggcttttcac tcttattaca acgactgatg tcgcgtctca tgggtcagca gcagggcgat
    46201 taacgtcaag ccagccatcg ccgccagata taaccccacc gcacctaacc cgtagttagt
    46261 ctgcaaccag gctgcgatat acggcgcaac ggaagccccg agaatcgacg ctacgttgta
    46321 agagaacgat gctccggtgt aacgcacttc tgtcgggaac agctctggta acagcgcgcc
    46381 catcggaccg aaggtcagcc ccatcagact taaccccagc agcaggaagg caaaaaccag
    46441 aatcgggttg ccagaaccga gcaacgggtt aaaggcgaac agcgcgaaca ggatgatcag
    46501 cgtggtgatg attaccatgc ttttacggcg accaaaggca tcagccagta atccggcgac
    46561 tggcaccatc acgccaaaac caataactgc catcatcagc atccacaaca cttcgttacg
    46621 cggcaggcca agcccaacgg gcgcggcggc ggtactaaag gtcatagagt agaccgtcat
    46681 gatgtaaaac agcgtatagg ttgccagcat aatgaacgta cccagtacgg ttacgcgaac
    46741 atgtttggtc agcagcgtac ccagcgggat cttcacctgt tttttcgctt tagcgacttt
    46801 ctcaaacacc ggcgactcat gcagcgacac gcgaacatac aggccgataa tgaccagcac
    46861 cgccgagaag ataaatggca cacgccagcc ccagctcata aactgctcgt cggtcagtag
    46921 ccaggaaagc agcaagaaag tgccgttggc aaagaagaag ccaatcggtg cgcccagctg
    46981 cgggaaggag ccgtacaatg cacgtttgcg cggtggggcg ttttcagtcg ccagcagagc
    47041 cgcgccgccc cattcaccac ctaagcccag accctgacca aatcgagcca gcgccagcag
    47101 cagcggggcg aaaataccaa tcgtggcata gcctggcagc aggccaatca ccacggtcga
    47161 aatccccatc gttagcaacg aggcgaccag cgtcgcttta cgcccaacgc gatcgccaaa
    47221 atgaccaaaa acggcagagc caatggggcg cgcgacgaag gcgatggcaa aggtggcgag
    47281 cgactgtagc gttgctgccg taggatcgcc ctgcgggaag aagatatgcg gaaacacaat
    47341 aacggccgca gtggcgtaaa tgtaaaagtc gaagaactca atggccgtgc caatgagaga
    47401 ggcgacaagg actttattac gcgagttgat cggcgtgtat tcttgctcgt ggtcgagtgt
    47461 tgtggctgtt gcttgcataa tcttttctta ttttttggct aacgaatagc catattacgc
    47521 acagcaaaag tgatatttca atgtgtaaca aatcgcggaa atggtcaaaa accaggcaaa
    47581 aaggggataa tgtttaggcc agtgattttt cttctatgaa atgcttcaca taattttata
    47641 aatagtggat aaaactggtt tcaggttaaa taaaagttac caactggatt tatatgctgg
    47701 ggttatgagt tggtatgaaa ttgtttgctc ctgaatagat ggctaaagag gctctgggaa
    47761 agagtttcaa gaggttatgt gcgcattata gccggatgcg atgctggcgc atcttatccg
    47821 gcctacgggg agccaggccg aataaattcg tacgccgcat ccggcctatt ttaaggctgc
    47881 gttttccccg gcattcgcgg gtcgtcttta tattcggcgg tggcaatcca cgccgcgcaa
    47941 aacagcgtca aacgggcgaa gaagtagaaa aatgccatca gtcccagcac ggaaccaaat
    48001 gctgcgccag agggggattt catcagcgat ggcagggtgt aggtcatcac gattttaatc
    48061 acttcaaaac caatcgcagc cagaaatgtt ccgcgaatca gggctttttt gcgcggacgg
    48121 tggcgcggca agcgccagaa gatccaaaag aaaagcagat agttggcgaa gatggaaatc
    48181 gccaggccaa tcaaccgcca cgtcggtttc agccactcaa tgctgttcag gtgcagggcg
    48241 ctaataatca tttgctgcgc cgaaccggca accgaggtga tcgaaagcgt cacaatcagc
    48301 gcaatcaaca aaccaatcag cgaaataaaa tcacgcagat attttaccca gaacttctcc
    48361 tgatcttgcg gcgagcgttc ccagacatcg cgtgactggg cgcgaatcgc ttcacgcagg
    48421 ttacccatcc agttgatgcc ggaataaagc gccaccgcca ggccgacaag ccctacggta
    48481 gtacgctgct gaacggcggt gttgatggtg tttttcaacg tggcggctag cgtcggatcg
    48541 ctgatgtttt gcagaatttt gtcgaagata tcctgtagca acatcggatg ggaggccagc
    48601 acaaagcccc ctgcggcaaa cgataccatc aaaatcggga tcatcgacaa aaatgagaaa
    48661 taggtgatag ccgcaccaaa ctggttaccc agccgatcgt taaagcgctc tgttgcgcga
    48721 atcaggtgcg caataaccgg ttggcgttgg actttctcgg cagtagtggt gacggtttcc
    48781 agcgcctggc taactttgcc gcctttctcg ctattatcca actgcttaat cggctcgtgc
    48841 tccagatcct gggtgggacg tttgatctcg ttttcctgcg tcatccgctc ttttgtcctt
    48901 ctgtcgtggt cattcttaaa aagtatagtc ggtcagtcca ggtgctcttt cattacgcca
    48961 gccagccact gcataaacag gtttacacgc cgggaaagct cccgacgctg cggataaacc
    49021 agtgaaaggg agagcggctc ggcacggtag ccaggcaaca cttcaataag ccgcccggca
    49081 cgcagggctt cgcgcacggc aatgcgcggg atctgaataa tccccagacc ggcaagaccg
    49141 gcggcgagat agttttcgcg actgtttacc gtcaacatgc cgccagactt aaaccactgg
    49201 acgccattaa cgctggcaac ctcaaaacct aacggatgta cacccaggtg cggtgtataa
    49261 cgcactattg cgtgtgaagt cagatcatcg gggctttgcg gataaccaaa gcgcgtcaga
    49321 tagtgtggac tggcacagtt gaccatggtc agtttgccga gagaacgggc gataacgcca
    49381 tcttccggta atgcgccagt gcgtatcacg caatcaaaac catcatgaag aatatctacc
    49441 ggacggtcat ggctactcag ttccagctca attcccggat gcagatagag aaattccgac
    49501 aagcgcggca gtaacaggct tttcgcgatt cctggcggga tgtcgatgcg taatttaccg
    49561 ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt cgctgagatt actcaacaca
    49621 tcttttgccc gttgataata ggtcatgcct tctggcgtga gtttgacccg tcgcgtagtg
    49681 cgctggagaa gctgggtgcc aagttgatgt tccagtgcct gtatctggcg cgaaacactt
    49741 ccctttggca aagcaaagaa atccgccgcg cgggaaaaac tttccagctc cgcgactttg
    49801 atgaacaact gcattgcgtg aattttatcc atttctttga ccgattgttg ttcacagcga
    49861 aacagtaacg cgcatatcgc attgtttatt gttttttcta acatctaaag ccgggtttca
    49921 caatgttgtc atgacgaatg tggcgatttc tcagtaggaa aagggaggaa tagctttctt
    49981 agaaaggtga atccccttaa acagagcgta tttttcagcg attcccttta aagtgaatag
    50041 aggacgtatt ggctatttgt gcttattttt gtctcattgc catttgttat tttggcttgt
    50101 ggtcgcggaa tttcattttt aattgcatgc gtactaacca ctaatataag gaaatcctca
    50161 aaattgtttt aaatagtgcc ctcgacatgt aatttcaggc gtttagctgt aactatttat
    50221 atcggtaact attttctacg cgtcatctct gtagaatggc caccgccaaa tggcgaattc
    50281 ttattttttc tcgcgggtaa tacccttcca ctacttaagt ctttttaacg tattcaggtg
    50341 gagattcagt tatgcaaata gtcatgtttg acaggcagtc aatatttatt catggaatga
    50401 aaatcagttt acagcaacgt attccaggag tgagtattca gggggccagt caggcagacg
    50461 agttatggca aaagctggaa agttaccctg aagccttagt gatgctcgat ggcgatcagg
    50521 atggtgagtt ttgttactgg ttgctgcaaa aaaccgtggt gcaatttcct gaggttaagg
    50581 tgttaattac ggcgacggat tgcaataaac ggtggttaca ggaagttatc cattttaatg
    50641 tgctggccat tgtgcctcgt gattcaaccg tcgagacatt tgcgttggcg gtaaatagtg
    50701 cggcgatggg gatgatgttt ctgcctggag actggcggac gacgccggaa aaggacatta
    50761 aagatctcaa atcgttaagc gcccgccagc gggagatttt aaccatgtta gcggcgggcg
    50821 aatcaaataa ggagatcggc agggcgctga atatcagtac cggaacggta aaagcgcatc
    50881 tggaatccct gtatcgccgt ctggaagtga aaaatcgcac ccaggcggca atgatgttaa
    50941 atatctcctc ctgaagagga taattttaca gccagcagga agttagcggc tggctgtaaa
    51001 aatattatgg ttcaccgtac aaaccaatta aacggcgcac cacaccgtta gtccagccaa
    51061 acccatcctg caacggatac tcgccaccgc cgccttcgcg gggaacacca tcggcaatat
    51121 ggtatttctc gatcagtttg tgctgttcca gatagaactg attcaccgtc ttcagccagc
    51181 tgcgcgcgat ttcatcaccc agaaggtcat cgccatacat tttaaatccc tgaatcgcca
    51241 tccattgtaa cggtgcccag ccgttgggtt tatcccactg ttcaccggtt tcgtactcgc
    51301 ttgccagaat cccgccaggt gtcagtaatc ggctgcgcac ggcgtttgcc agacgatcgg
    51361 cctgttcatg gttcgccata ccgacataga gcggcacaat ggcggcagcg gaaaacagcg
    51421 ccagttgctc gcgtcgccag tcgtaatcgc ggtagatacc gttttcatca tcccagaggt
    51481 aacggtttac cgcatcgcga cgggcacagg ccttctggcg gaacagtgct tctgtctctt
    51541 tctcgccttt cagcgccgag atgttggcga tggcgctctc cagtttaaac aggaaggcat
    51601 tcagatcgat ggggatgaac tgggtggtac gaatgctcgc cagacgacca gtatcacgca
    51661 gccagcggga agagtaatcc caaccggagg ccgcccccgc gcgtaaatcg cggtacacct
    51721 cgttgggcgg gcgaccagaa tgtttcgcgg tttcaacgtc ctcaagccag gattcgtcac
    51781 gcggtgtgtc gcgatcgtcc cagtaacggt tgagcagcga tccgtccggc atccgcacaa
    51841 catggcgata ggcctgatta gggatcaacg attctgcacc gtccatccag aaggcatatt
    51901 ccattttcag gtggtcgaga tagcggcgcg caccgcgtac accatcttct tcaaacaact
    51961 ccaccatcag cgcaaaaacc ggtggttgcg agcggctcaa ataataggtg cggttgccgt
    52021 tggggatgtg accgtagttt tcgatcatcc aggcgaagtt atcggccatg catttcagca
    52081 aatcttcccg accactttcc gccagcccca gcatggtgaa ataggaatcc caatagtagg
    52141 tttcgctaaa acggccgccc gggacaatat atgactgcgg cagcgccagc agagaagacc
    52201 acggaatgtg atcctgcggt tcgcgggtta gcaccggcca cagctggtcg atatgctctt
    52261 tcagggaatt ttgcgggtcc gatacatact cgctggagta gacctccggc agccagaagt
    52321 ggttttcaac aaacttgcgc aagtcaaaat cacgatgacg gcgcacttta cggtagcgga
    52381 ttaagatatc cagcgggtcc attttaggtg cgcagtcggg aaaggtttta ctgtcgggga
    52441 aaattttcgc cgactgaaca tgttcgaaca gttcgagata gcgatcggca ggcgtcagcg
    52501 catcagaggc gggcagccct tcaatcatct cgggttccgg ttctgcctcg atcatctcat
    52561 ccagttttaa ttcatatggg tccagttcat agcagagatc gacctcgatc atcagttcgt
    52621 ctggattagg gttttgaatt ttctgattga gcataaggat ccgacctccg aaattcgtcg
    52681 ctaattatcg ggcttttctt taagggtagt cattcgctcc gcctggtggg cgacaaagcg
    52741 tgcggtggat cacgtttatt aacctgccaa aaaatataac gcactaatat caatggaatt
    52801 tataaaaata cggatttcgt gttgtttttc attttcagaa cattcaatcc tgctctcgcc
    52861 gcaatcatga tatgccattc ctgatctggg taataggcac tggctatctt attgatagtt
    52921 tatattcatg taattgattg attcctatca aaaagcatat ctccctgttt tttatgatgg
    52981 ctttgtctcg cagagacgtt tgcagcattc tggaaaattt tctgggtcac aataactcac
    53041 caccgtgagg gaagaataat gaaaatggtc tcacgtatta ccgcgatcgg cctggctggc
    53101 gtcgcgattt gctatttagg gttatctggt tatgtgtggt accacgataa taaacgcagt
    53161 aaacaggccg atgttcaggc atctgctgtc agtgaaaata ataaggtttt aggctttctc
    53221 cgtgaaaaag gatgcgacta ttgccataca ccttcggcag aattacccgc ctattattat
    53281 attcccggcg cgaaacagtt gatggattac gacataaagc ttggatataa atcttttaac
    53341 ctcgaggccg tgcgtgcggc tctgctggct aataaacccg tttcgcaaag cgatctgaat
    53401 aagattgaat gggtgatgca gtatgaaact atgccgccaa cgcgttatac cgcgctacac
    53461 tgggcgggta aggtgagtga tgaagagcgg gcggaaatac tggcctggat tgcaaaacag
    53521 cgcgcggaat attacgccag caatgatact gctccggagc atcgcaatga accggtgcag
    53581 cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg cgttgggttt tgcgctgtat
    53641 cacgatcccc gtttatcggc tgatagcacc atttcatgcg ctcattgcca tgcgttgaat
    53701 gcggggggcg tcgatggcag aaaaacatcg attggtgttg gtggcgcagt tgggccgatt
    53761 aacgcgccga cggtatttaa ctcagtattt aacgttgagc agttctggga tggtcgtgcg
    53821 gcaacattgc aggatcaggc tggtggaccg ccgttgaacc cgattgaaat ggcgtcgaaa
    53881 tcctgggacg aaattattgc taagctggaa aaagatccgc agcttaaagc gcagttcctc
    53941 gaagtctatc cacaaggttt cagtggcgaa aatattactg atgccattgc tgaatttgag
    54001 aaaacattaa ttacgccgga ttccccattt gataaatggt tgcgtggaga tgagaatgct
    54061 ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag ataataaatg tgcaacttgt
    54121 catggtggta ttattctcgg cggacgttcc tttgaaccgt tggggctgaa aaaagacttt
    54181 aactttggtg aaattacggc ggcggatatt ggtcgtatga atgttactaa agaagagcgt
    54241 gataaattgc gtcagaaagt acccggttta cgtaacgttg ctttaacggc accgtacttc
    54301 catcgcggtg acgtgccgac gctggacggg gcggtgaaac tgatgctgcg ctatcaggta
    54361 ggcaaagagc tgccgcagga ggatgtggat gatatcgtag ctttcctgca cagtctgaac
    54421 ggggtttaca cgccgtatat gcaggataaa caataattaa tttgatcgcc cgaacaggag
    54481 tgtttgggcg atttttatta cgataataaa gtctgttttt aatattatca tatta
//
LOCUS       DAZUCC010000005        54358 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZUCC010000005 DAZUCC010000000
VERSION     DAZUCC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.005"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..162
                     /gene="yhfL"
                     /locus_tag="ACHY09_000221"
     CDS             <1..162
                     /gene="yhfL"
                     /locus_tag="ACHY09_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFS3883243.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
     gene            complement(269..1354)
                     /gene="yhfS"
                     /locus_tag="ACHY09_000222"
     CDS             complement(269..1354)
                     /gene="yhfS"
                     /locus_tag="ACHY09_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS3883244.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAPAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVSRIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     YYAIRINPNRSGEETVLRILRESIASI"
     gene            complement(1366..2670)
                     /gene="yhfT"
                     /locus_tag="ACHY09_000223"
     CDS             complement(1366..2670)
                     /gene="yhfT"
                     /locus_tag="ACHY09_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFS3883245.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2682..3035)
                     /gene="yhfU"
                     /locus_tag="ACHY09_000224"
     CDS             complement(2682..3035)
                     /gene="yhfU"
                     /locus_tag="ACHY09_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFS3883246.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3046..3924)
                     /gene="php"
                     /locus_tag="ACHY09_000225"
     CDS             complement(3046..3924)
                     /gene="php"
                     /locus_tag="ACHY09_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFS3883247.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNIVKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(3921..5147)
                     /gene="yhfW"
                     /locus_tag="ACHY09_000226"
     CDS             complement(3921..5147)
                     /gene="yhfW"
                     /locus_tag="ACHY09_000226"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS3883248.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPMLEKMGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALLNADWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITFNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVLTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(5147..6310)
                     /gene="yhfX"
                     /locus_tag="ACHY09_000227"
     CDS             complement(5147..6310)
                     /gene="yhfX"
                     /locus_tag="ACHY09_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFS3883249.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPLAHQ
                     GHLVQIPCHQVSDAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVADIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(6394..6756)
                     /gene="yhfY"
                     /locus_tag="ACHY09_000228"
     CDS             complement(6394..6756)
                     /gene="yhfY"
                     /locus_tag="ACHY09_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS3883250.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVKPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6773..7678)
                     /gene="yhfZ"
                     /locus_tag="ACHY09_000229"
     CDS             complement(6773..7678)
                     /gene="yhfZ"
                     /locus_tag="ACHY09_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFS3883251.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVNRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            complement(7968..8972)
                     /gene="trpS"
                     /locus_tag="ACHY09_000230"
     CDS             complement(7968..8972)
                     /gene="trpS"
                     /locus_tag="ACHY09_000230"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS3883252.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8965..9723)
                     /gene="gph"
                     /locus_tag="ACHY09_000231"
     CDS             complement(8965..9723)
                     /gene="gph"
                     /locus_tag="ACHY09_000231"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFS3883253.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9716..10393)
                     /gene="rpe"
                     /locus_tag="ACHY09_000232"
     CDS             complement(9716..10393)
                     /gene="rpe"
                     /locus_tag="ACHY09_000232"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS3883254.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10411..11247)
                     /gene="dam"
                     /locus_tag="ACHY09_000233"
     CDS             complement(10411..11247)
                     /gene="dam"
                     /locus_tag="ACHY09_000233"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFS3883255.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11354..12640)
                     /gene="damX"
                     /locus_tag="ACHY09_000234"
     CDS             complement(11354..12640)
                     /gene="damX"
                     /locus_tag="ACHY09_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFS3883256.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12732..13820)
                     /gene="aroB"
                     /locus_tag="ACHY09_000235"
     CDS             complement(12732..13820)
                     /gene="aroB"
                     /locus_tag="ACHY09_000235"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS3883257.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13877..14398)
                     /gene="aroK"
                     /locus_tag="ACHY09_000236"
     CDS             complement(13877..14398)
                     /gene="aroK"
                     /locus_tag="ACHY09_000236"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFS3883258.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14799..16037)
                     /gene="hofQ"
                     /locus_tag="ACHY09_000237"
     CDS             complement(14799..16037)
                     /gene="hofQ"
                     /locus_tag="ACHY09_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFS3883259.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15949..16353)
                     /gene="hofP"
                     /locus_tag="ACHY09_000238"
     CDS             complement(15949..16353)
                     /gene="hofP"
                     /locus_tag="ACHY09_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFS3883260.1"
                     /translation="MRVKRWLLAGITLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            complement(16343..16783)
                     /gene="hofO"
                     /locus_tag="ACHY09_000239"
     CDS             complement(16343..16783)
                     /gene="hofO"
                     /locus_tag="ACHY09_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFS3883261.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16767..17306)
                     /gene="hofN"
                     /locus_tag="ACHY09_000240"
     CDS             complement(16767..17306)
                     /gene="hofN"
                     /locus_tag="ACHY09_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFS3883262.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQLQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(17306..18075)
                     /gene="hofM"
                     /locus_tag="ACHY09_000241"
                     /pseudo
     CDS             complement(17306..18075)
                     /gene="hofM"
                     /locus_tag="ACHY09_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
     gene            18195..20747
                     /gene="mrcA"
                     /locus_tag="ACHY09_000242"
     CDS             18195..20747
                     /gene="mrcA"
                     /locus_tag="ACHY09_000242"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFS3883263.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20915..21475)
                     /gene="nudE"
                     /locus_tag="ACHY09_000243"
     CDS             complement(20915..21475)
                     /gene="nudE"
                     /locus_tag="ACHY09_000243"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFS3883264.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            21795..23930
                     /gene="igaA"
                     /locus_tag="ACHY09_000244"
     CDS             21795..23930
                     /gene="igaA"
                     /locus_tag="ACHY09_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFS3883265.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAHDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23995..24663
                     /gene="yrfG"
                     /locus_tag="ACHY09_000245"
     CDS             23995..24663
                     /gene="yrfG"
                     /locus_tag="ACHY09_000245"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFS3883266.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24674..25075
                     /gene="hslR"
                     /locus_tag="ACHY09_000246"
     CDS             24674..25075
                     /gene="hslR"
                     /locus_tag="ACHY09_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFS3883267.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            25100..25978
                     /gene="hslO"
                     /locus_tag="ACHY09_000247"
     CDS             25100..25978
                     /gene="hslO"
                     /locus_tag="ACHY09_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS3883268.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(26116..27840)
                     /gene="yhgE"
                     /locus_tag="ACHY09_000248"
     CDS             complement(26116..27840)
                     /gene="yhgE"
                     /locus_tag="ACHY09_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFS3883269.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRRRGLLMAFDGVQRLQQQISEKA
                     LADNVFIASGSGKPDTIFWSAVMKNRYNAMICIEKDACVLVEQDLNSDGRAERILFAF
                     NDERYIIYGIDPAGKEWKEITMNSLPAVITKEKLLMAAEDGKLGTKPKVWRDLTVDGE
                     RLDLNLNE"
     gene            28219..29841
                     /gene="pckA"
                     /locus_tag="ACHY09_000249"
     CDS             28219..29841
                     /gene="pckA"
                     /locus_tag="ACHY09_000249"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS3883270.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTRILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            29956..30273
                     /locus_tag="ACHY09_000250"
     CDS             29956..30273
                     /locus_tag="ACHY09_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3883271.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            30332..30628
                     /gene="nadS"
                     /locus_tag="ACHY09_000251"
     CDS             30332..30628
                     /gene="nadS"
                     /locus_tag="ACHY09_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFS3883272.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(30660..32012)
                     /gene="envZ"
                     /locus_tag="ACHY09_000252"
     CDS             complement(30660..32012)
                     /gene="envZ"
                     /locus_tag="ACHY09_000252"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFS3883273.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGSSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(32009..32728)
                     /gene="ompR"
                     /locus_tag="ACHY09_000253"
     CDS             complement(32009..32728)
                     /gene="ompR"
                     /locus_tag="ACHY09_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFS3883274.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32956..33432
                     /gene="greB"
                     /locus_tag="ACHY09_000254"
     CDS             32956..33432
                     /gene="greB"
                     /locus_tag="ACHY09_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFS3883275.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            33529..35850
                     /gene="yhgF"
                     /locus_tag="ACHY09_000255"
     CDS             33529..35850
                     /gene="yhgF"
                     /locus_tag="ACHY09_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS3883276.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            36288..36515
                     /gene="feoA"
                     /locus_tag="ACHY09_000256"
     CDS             36288..36515
                     /gene="feoA"
                     /locus_tag="ACHY09_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFS3883277.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            36532..38853
                     /gene="feoB"
                     /locus_tag="ACHY09_000257"
     CDS             36532..38853
                     /gene="feoB"
                     /locus_tag="ACHY09_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFS3883278.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            38853..39089
                     /gene="feoC"
                     /locus_tag="ACHY09_000258"
     CDS             38853..39089
                     /gene="feoC"
                     /locus_tag="ACHY09_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFS3883279.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGNCKSCPEGKACLREWWALR"
     gene            39292..40173
                     /gene="rpnA"
                     /locus_tag="ACHY09_000259"
     CDS             39292..40173
                     /gene="rpnA"
                     /locus_tag="ACHY09_000259"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFS3883280.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKESLMTIAERLRQEGFQKGIRAIAKTMLDAGVPLEDV
                     LRFTSLTVEDLAEINHQ"
     gene            40245..40364
                     /locus_tag="ACHY09_000260"
                     /pseudo
     CDS             40245..40364
                     /locus_tag="ACHY09_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(40393..41163)
                     /gene="bioH"
                     /locus_tag="ACHY09_000261"
     CDS             complement(40393..41163)
                     /gene="bioH"
                     /locus_tag="ACHY09_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFS3883281.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKMVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            41201..41884
                     /gene="gntX"
                     /locus_tag="ACHY09_000262"
     CDS             41201..41884
                     /gene="gntX"
                     /locus_tag="ACHY09_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFS3883282.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            41943..42518
                     /gene="nfuA"
                     /locus_tag="ACHY09_000263"
     CDS             41943..42518
                     /gene="nfuA"
                     /locus_tag="ACHY09_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFS3883283.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42879..44195
                     /gene="gntT"
                     /locus_tag="ACHY09_000264"
     CDS             42879..44195
                     /gene="gntT"
                     /locus_tag="ACHY09_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFS3883284.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(44240..46324)
                     /gene="malQ"
                     /locus_tag="ACHY09_000265"
     CDS             complement(44240..46324)
                     /gene="malQ"
                     /locus_tag="ACHY09_000265"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFS3883285.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTFTQEDQRVHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQTRDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QVAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGSETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(46334..48727)
                     /gene="malP"
                     /locus_tag="ACHY09_000266"
     CDS             complement(46334..48727)
                     /gene="malP"
                     /locus_tag="ACHY09_000266"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFS3883286.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELAEYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWTNDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPQVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            49349..52054
                     /gene="malT"
                     /locus_tag="ACHY09_000267"
     CDS             49349..52054
                     /gene="malT"
                     /locus_tag="ACHY09_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFS3883287.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTDDWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWHNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(52097..53113)
                     /gene="rtcA"
                     /locus_tag="ACHY09_000268"
     CDS             complement(52097..53113)
                     /gene="rtcA"
                     /locus_tag="ACHY09_000268"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFS3883288.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSSTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(53117..54343)
                     /gene="rtcB"
                     /locus_tag="ACHY09_000269"
     CDS             complement(53117..54343)
                     /gene="rtcB"
                     /locus_tag="ACHY09_000269"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFS3883289.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        13037 a        14936 c        13607 g        12778 t
ORIGIN      
        1 aaatttatta aagttgcact ggtaggtgcg gtactggcta cgttaactgc atgtactggt
       61 catattgaaa accgtgataa gaactgctct tacgactacc tgctgcaccc ggcaatttct
      121 atttctaaaa tcattggcgg ttgcggtcct actgcacagt aagtctctga ctgaaagcgg
      181 ctaacaccgg tttaattgcc tgacgccctc ggggttaccc gagggcgtat ttatttctgc
      241 attcttttcg aaaacatcat gcacaagatt aaatactggc aatactctca cgcaaaatcc
      301 gcagcaccgt ctcttcaccg ctgcgatttg ggttaatacg aatcgcgtaa tattcgagtt
      361 gcggattcgc ctggcgaaac gttccggaaa gtcgataaaa aagtggcgga atttcatatt
      421 tcgactctgc accaaccggg taaggcaagg caccgcgctt ttgcgcctct tccagtactc
      481 tggcggcaat cggctgatga aactccacaa tcaacacctt cgactgcgca ttggcaatca
      541 ccgcgctttt cacttccgcc actgcgccac cgttaagtaa agccagcaac cgttcagata
      601 cccccgcctg caccgcgtgc atcactggcg cgagcaccag accacgcaac acctctaatg
      661 cctgcgcccc ctgaatctga ctgccgccgg aatagagcgt ggcgcgaatc cggctaataa
      721 catcggcatc gcctaccacc gcaccaacgc cctctggccc aaatagcttg aagcaggaaa
      781 atgtcgacac attcgccccg cattcgcagc caattcgcga caccttcatc accgcatagt
      841 tgtcatcggt taacgctgga acacctgccg cgcgcaacgt tgccagcacg tctgccagca
      901 catagcggtc ctgcggctgc tggcgcgtat gctgcaccag cgcggcatca ggttgttgct
      961 cgtcaacaac ctgcttcagt gccgacagat cattgaaatc agccgtaatg agcgttagcc
     1021 ccatctgctc aataatgacg cgcgtcgttg gataaaccgg cgcatcatgc accagaagac
     1081 gttgccccgg tttgagcaaa gccgccagtg ccgcacgaat cgcgccagta cccgcgccct
     1141 gtaccagtgc cgccgcgggt gcatgaaacg cgtcagccag cacctgttcc acacgctggg
     1201 taatgcgggg ctgatttagc cccggcgtta agcccaaatc accgcaggta agaaactcgc
     1261 tgccaggaaa atgacggcaa atcgtgtcca ccagcgcaaa ctgcttttgc tgcgcctcaa
     1321 taagcgtcag gctttgcaga ggaaacgtct tcataggtgc cttccttaag ccggaacgaa
     1381 caggccaagc cagtaaagaa cattcagcag gatgccggtg atcattactg ctacgaccgg
     1441 tgccgccatt ttctgtaccg gacggcccag ggattcgttg aggaagtaaa tggcaaccgc
     1501 gatagagaat ccggtatagc ccgccatttt gattgctgca aagatcgaac cgaccagcaa
     1561 cgccacttcc atcagcatat tcatggcgtt acggatgtta tccgacgcat tacgtaccga
     1621 cgggtagcgc cccaaccatt tgccgatcga acgaagcagc aagacttccg ccgaaataac
     1681 cactgcgcct aataccgctg caaccatcgg attcggcgag agatagccca ccgcataaac
     1741 aaaggtaaag cccgcaactg catacacacc agtggctaac gcggtggtgg caatcaacgg
     1801 tacaaaaccc agcccgcgca taaattccgc cagagctgcc tggttgatta gtgtttgcga
     1861 ttgttccggc gttacgcctg cggaatatgc tttctccagt gtgaagatcg acacttcact
     1921 gccagcaaaa atcttcatgc tggcaacggc ggcaatcagc gccccgacaa tagcgatata
     1981 cggtaagttt ttgataatcc gtgacgtgcg ttcttcaaac accgaaagcc cgctggcatc
     2041 aatgtcattt tcatcacgat gacgcaggtc gtgagtaatc gcgatcccca gcagcatcac
     2101 catgccaata aagatttcga tggattccgg attgagatgc gggaaatagc gcactactac
     2161 cacacgggtc atcagtacca ccacggcggc gatcagactt tgcttccagc caaactggta
     2221 gaaaatcgcc accaacggga acagtgcaaa agctgaaacc accggcgagc ttaattcccc
     2281 caggctacct aatacatcca ccggtagcgc ggtcagcagc tggtttaccg gtaacaggca
     2341 agtaaggatc aacacgcccc agatagcgcc aagaccaaac gccatcaggc tgtttatcgc
     2401 cagtacgccg aggatatcgg taggaagaaa gagtaaccat gcgttgagca gtccggtttt
     2461 cagggtaaaa gagatcccca ccgaagccac aaaaccaatg ctcagaccaa aagcgatact
     2521 ccccgcctcg cgacggttca tatagccttc aatcagttgc ggcaggatcg ggcggatacc
     2581 gtcatgaaaa acggccgccg agcgatgcgc cagaagcgat gtcatacccg tcaggcacgc
     2641 caccacgata atctgaatat acagatccat agtcgtgcct tttatttcag atggttaatc
     2701 agcatcggaa tcgcgtgttc gacgtgctca acggaaagcc caaacgccac tttcccttcg
     2761 gcgatcattt tggcgatatg ttcgtctttc gctttaatgc ctggtttggc aatggtgcaa
     2821 cttttgttat agccaatcac cgcgatggca atcgacaatg ccgcgcccgc accggtatta
     2881 cacgcgccga tgtagtaatc cagttgcccg gatttgactt tcattgccgc ttccatgtcg
     2941 ttgtgaatga aaacttcaaa acagccagga gccgtcgctt caatggtttt tttaatctgc
     3001 tcacgctgta agcctgcaac accaatcttt ttcatgagtc tgtccttatt ggaaaaattg
     3061 agagggagtt tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa
     3121 ttgcggaata aaggtggtca ataaatagtc atagccataa ccaccgttgg cttttaaatg
     3181 ggagcggcgc gtaatatcca tcgacagcat gacgcggtta agcaaacctc ggtcacgtag
     3241 cgcgtggagc atcgcaatgc gcttttcgtc cgggtagtaa ctgttcttac cgatggtgtc
     3301 gaactgcaca tacgcgccga gatcgatcat cttcacaatg ttgtcgaggt tgtctttcag
     3361 atcgcagtga ccaacggtga cgcgcgaaag atcaactccg tgggcttgca gtagcgccag
     3421 ttgttccagc cccatggtgc taaaagaggt atgcgtggag atcgggcgtc cggtctggtt
     3481 atgcgccagc gcggcggcaa taaatacctt ctcttccagc ggcgtgattt ttccttcgct
     3541 ggagcctatc tccgcgatga tcccggcttt cagctccgtg ccatcgatac cctgttcaat
     3601 ttcatcgacc atctcttgcg ccagttcctg cacgctgcgg gtcgccacat gttccgggaa
     3661 aaacgcgtcc tggtaataac cggtacaggc caccacgttg atcccggttt cgcgcattac
     3721 atcgagcata aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg
     3781 cacgccctgg gccatcaggt cgttcatctc ctggcaaatg aacgcatact gatcaaggcg
     3841 gcagtccacg ttgtttttaa agccggagag atcaatatgc agatgctcat gggcgagggt
     3901 gtagcccgtc ggatcaaaac tcatagggtg tctcctgcaa accggaggga ggaaagaaaa
     3961 gagcgaccat tttgtggcgg tggcgcgcgg aaaaattcac atacggtagc ccccacatca
     4021 gaaagcgtgg tgagcacacc aagctgcgta gcgaccatcc cttgctgata aaccagcact
     4081 ggcaccactt cgcgggtatg gtggctgtga ccaatggtcg gatcgttacc gtgatccgcc
     4141 atcacgacca ggcaatcatc tggctgcatc gcctcaataa gccgggcaag gttacggtca
     4201 acgacctgca aacgttccgc ataacgtgcg acgtcttctg catgaccagc gaggtcggtt
     4261 tcctggatat tggtacaaat aaacgccgtc ggatgggtgt taaattcgtt gaaggtgata
     4321 tccataatcc gctggctatc caccaggttt tgccagctca cgccataagg attactggca
     4381 atatccgcca ctttccccac cagcacggtt ggtacgccca cttcatacag tttttgtggc
     4441 acctgcactt tttcatcgac gccgtagccc atatgcacga cctggaagcc gttgtcataa
     4501 gcgccagaac gcggcgcatt gatgccaata aagcgccctt ctttgctttc tgcggcatcg
     4561 aggatgcgct ggctgtcggt taacaggcca ccaaatgtaa tgacccgacc gacctgtacc
     4621 tgctcacgca cgatacgacc aattttgatt gcgtcgtcaa aagagatcac agagagattg
     4681 gcggtgatgt tatagacctg acctaaatcc gcctcgagat tatcgccaat cgcaaccgcc
     4741 tgattgaccc acagaaattg cagatcatcg ccacggcgct ccacctgcca gtcagcgtta
     4801 agtaatgctt gctcaacacg gtcaatcaca tcgcgaaagg gcatccgtag cggcggtaac
     4861 gggcgcgtgc ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc
     4921 tctgccacgc cccaggttgc ggagtctgac ggctgcatat ctccaggcgc ataacctaat
     4981 gcgttgatta gccccatttt ctccagcatt ggtagctgca aatgcggcaa ctggctcagg
     5041 atgtgaccac aggtatttgc cccggcatcc tgcggacgca ccaacgtgac atctttcatt
     5101 gcccctacgc caaagctatc aatcactaac accacaaatc gcgccattac gcccccagag
     5161 agtttccaag actgtcataa cgaccgacga tttctggttc gccacggtga ataccggaca
     5221 gcaacaccac atcgctacgg gtgacaaaaa tctgcgtgcg aaaacagagc actactgcac
     5281 tgcttaccgg aaactcgcct gccagcggca gggtgtagtc gatactgctg tcatccacag
     5341 ttttgagatt ggtttcagta atcttttgat tttctggcgt aaacaccagc gcatgttgcg
     5401 cgtgaccacg acggtagtaa ccgccgccgt agcagtagct gtcgccacga aaatgatggg
     5461 agatttcact taaccagagc atcgcgatac gttcaggctg atcgccctgc tggtttgccg
     5521 gaatcgtccc cgtcagcgca tgaccgggtt cggcatgagt cacaccgtat tccgccagca
     5581 atggcagcga agtgcagctg gtcgcagaag gtgcgttcag ttgctcaatc gcaataccag
     5641 atttcgccag ttgatcccgt gcctgtatca gcgtgtgaag attcggtgtc ggcaaaactt
     5701 tcccggcagc ctcatcccaa agcaggcaag ggaaatgggt tagtccggct aaatgcagcc
     5761 ccggcagttt cttaatatca gcaaccacct cgtgcagcga atgctgtaca aaaccgctct
     5821 cctgccccgg ataaagaaaa tcatcgtcgc tataaacttt gagcagcaca gactgcacac
     5881 gtccggcctt caccgctgct gcagaaactt cccgcgcttt atcgagagta aacacggtga
     5941 tgacgtcagt tccctgtgca acggcgtcag aaacctggtg acaagggatt tgcaccagat
     6001 gcccctgatg cgccaacggc agaccggcgc ggcgcatgac ccgcgcctct ttgtaatcca
     6061 ccgccacaat gccgctgtag cccagtgcca gaagtttttc cgccagccac ggattacggc
     6121 caaactgctt ggtcatcaga tacagttcaa tgccgtaaag tcgcgccgtc tcaatcagcc
     6181 gcttaccgtt ttccagtatc tgatccacat cgatcaccca gctgtccggg gcgatcttgc
     6241 cctgctgcca caaacttagt gcggcggaaa tcagcgccgg gttctggcgt ttcaatgctt
     6301 ctacaaacat cttgttgcct cgtttctgtt ccttgaatta aatattcatt tttctgaata
     6361 tttagattat gcgacgtgga tttgtcggga gcttcaaacc tcttcgttag cagccatcca
     6421 taatccataa agattagcca gcaaatagcc ttcttcacac ggcttaactt ccagtgcgaa
     6481 ttccttcaac aacacctgat gcaattgcac aacggcttgc cagtggctgg attgcgccag
     6541 ctctgccagc aattcgtcat ccagcggctc tatttcttca ccacggcgac tgcgcatcag
     6601 tgcgctcgcc atatgtgtca tcgccatcat tccttgctcg ctgcgcaccg gtagatgaca
     6661 ctctgtttcc agtacgttga cgacctgcat catgcctttg cagatgtcct tatcaataac
     6721 gcctgcctca caaagcaggt tgagtctggt ttccatttca gatgcctttc gtttaataac
     6781 tcggttcctg ttccccactg accactcgtt gttgatgggc aagcagggcc tgtttatcaa
     6841 caactgcacg cagcaattgt tgcattgggt aatcatcgac tcgcgtcaaa actacggctt
     6901 cggtggcctg taaaaatcgc ggatcgtctg tgagcggcgt cgcttctaat cccagcatgg
     6961 tcagttcgtt ttccgccacc acgttccaga tcaccgcatc aacatcgcct ttaacaatgc
     7021 gttgtaaact ctcgtgataa gagagatcga ctcgttccac atcactatcg ccaaaaaaga
     7081 catcggtcat gattttctga tccgccgaac ggttatccag ccccacgcga ttcacgtttg
     7141 cggactcgcc tttacggcaa atcaactggt gctcgccaac gtaggtgtgc ggccccaact
     7201 ccagcgcgag gcataaccct ttttgcgtga gataactttc cgccgccagt cgggaaacaa
     7261 ccgccatgtc atacacgccg ttaagtaaac actccacgcg aatatccgcg ccgcgcatgt
     7321 gcgcatagta aaaaggaata ccatcaaact gagctttcaa tccgctcgcc aggccttcgt
     7381 acaaacgggt atagggcaag ggcatcgcgc acaccacatt gttgatatcc acatgactca
     7441 gcaatgcttt gttatccatt tccaccagat aactgccatt acgcccacgg cgttctatcc
     7501 gtatcgctcc gcttgattcc agcgttttca acgcggcctg cgtcaggcca acggatgaac
     7561 ggcattcagt tgccagttca tctatggttt tcagtcgatt accgcacttt tcacccaaca
     7621 aataacgggc cagtgtcgtg atgacgacgc cttctttttt gataaacgtt cgacgcataa
     7681 taaattttca gtaaattgaa tatttatatc ttcaggaatt tgaagataag gcgcaacagc
     7741 gagatgtgga aacggcgagg cacttcacat tttttcggat tattgcaaat gaggataaaa
     7801 aaaccgggtt tccccggttt cagagtgatg ataaaagcaa aattgcctga tgcgctacgc
     7861 ttatcaggcc tacatttcct tgcaatatgt gcattacttt gtaggccgga taaggcgttc
     7921 acgccgcatc cggcatgaac aaagcgcaat ttgccagcaa tagtgaatta cggcttcgcc
     7981 acaaaaccaa tcgcttcgta caccgctttc agcgtacgag aagcgtgtgc actggctttt
     8041 tccgcgccat ctttcatcac ctgttgcagg aaggcttcgt cattgcggaa acggtgatag
     8101 cgttcctgca attcagtcag cataccggaa acggcatcag ccacttcgcc tttcagatga
     8161 ccatacatct tgccttcgaa ctgtttttcc agttccggga tgctctggcc cgttaccgca
     8221 gaaaggatat ccagcaggtt ggaaacgccc gctttgttct gcacatcgta gcgaactacc
     8281 ggcggctcgt cggagtcagt gaccgcacgt ttgattttct tcactaccga tttcggatct
     8341 tccagcaggc cgataacgtt attgcggtta tcgtcagact tggacatctt tttggtcggc
     8401 tccagcagcg acattacgcg cgcgccagat ttcggaataa acggctccgg cactttaaag
     8461 atctcgccat acagcgcgtt gaaacgctgg gcgatatcgc ggctcagttc caggtgctgt
     8521 ttctggtctt caccgaccgg taccagatta gtttgataca gcaggatgtc cgcagccatc
     8581 agcaccggat agtcaaacag accagcattg atgttctcgg cataacgcgc agatttatct
     8641 ttaaactgcg tcatgcggct cagttcgccg aagtaggtat agcagttcag tgcccagcct
     8701 aactgtgcat gttccggcac gtgggactga acaaaaatgg tgcttttctc aggatcgata
     8761 ccacaagcca gatacaaggc cagcgtatcc agcgtcgctt tacgcagctt ctgtgcatcc
     8821 tggcgcacgg tgatcgcgtg ttggtcaacg atacagtaaa tgcaatgata gtcatcctgc
     8881 atgtttaccc actgacgcag cgcacccatg tagttaccaa tggtcaattc acctgagggc
     8941 tgtgcgccac taaaaacgat gggcttagtc atttttcgat tcctgatttt cgctatgcgg
     9001 aagccctaat gcgggcagaa ggtcatttat agactgataa attacatcag gctggctgag
     9061 atcgatagcc tcgccgtagt tatatccgta ggttaagcca actgatgggc aacctgccgc
     9121 ttttgctgcc tgaatatcat tgcgtgagtc gccaacaaac agcatctgtt gtggggcaat
     9181 tcccatccgc tcagccacca gtaacagcgg gtccggatgc ggttttttgt tttgcacatc
     9241 atcaccgccg ataaccacgc tgaagtattt ggcgatatct aaggcttcga gcagcggcgc
     9301 gacgaacggc gtcggtttgt tggtgaccag gcctagcggc aggcctttag cctgcaatgc
     9361 gcccagcgta tcggcaacgt gcgggaacaa aaacgtcccc tcttcggcaa cctcgccata
     9421 gtagcgatcg aacagtttac gcagaatacg tacctgttct tctgccggaa tgtcgtcatc
     9481 aacgggcggt ttacccattg ttttacgcag agtcgcacgt tcctgacgcg cccaggtcaa
     9541 tgcgcgctcc atcagaatat ctgcgccgtt accaatccag gtaataacgc gttcttcacc
     9601 tgcgacaggc aactccagcg catacagcgc catatctacc gcagcagcaa gaccgggtgc
     9661 actgtcaacc agcgtaccgt cgagatcaaa agcgacgccg cgaatatctt caaacttatt
     9721 catgacttac ctttgccagt tcactgcgca tttcatcaat gacttttttg tagtctggct
     9781 ggtcgaagat tgccgaacca gcaacaaaca tatccgcgcc cgccgcagcg atttcgccaa
     9841 tgttgttcac cttcacgcca ccgtccactt ccaatcgaat gtcaaagcca gactcgtcga
     9901 tacgacgacg tacttcgcgc agtttatcca gtgtttgagg aatgaaagac tgaccgccga
     9961 aaccaggatt gacggacatc agcaggatca catccagctt atccatcacg taatccagat
    10021 aactcagagg tgtcgccggg ttaaatacca gacccgcttt acagccattt tctttaatca
    10081 gttgcagcgt gcggtcaaca tgctcggagg cttctggatg aaaggtaatg atgctggcac
    10141 cagcggcagc gaaatcaggc acaatgcgat cgacgggttt caccatcagg tgtacgtcga
    10201 taggggcggt aatgccgtag ttacgcaagg atttcagcac cattggccca atagtcagat
    10261 tgggaacata gtggttatcc atgacgtcaa aatgcacgac atcagcgcca gctgccaggg
    10321 cttttgcggt atcttcaccc aggcgggcaa aatcagccga cagaattgag ggggcaatca
    10381 aatactgttt catccgcttc tccttgagaa ttattttttc gcgggtgaaa cgactcctgg
    10441 tttgtacaaa gccagcagtt cgtccacctt tttacgtgtg ccgccgttgc tgcttatact
    10501 gcgtcgaact ttgacgacat gcaattttgc gcgctgatac cactcacgcg ttaacatcgt
    10561 atcgtgattg gagatcagca ctggaatatg gcgatcaacc agaccttcgg cgatctccgc
    10621 cagatgcgct tgttgttcaa gcgtaaaact gtttgtgtga tacgccgtaa agttggcggt
    10681 cgcagacagc ggcgcataag gcggatcgca atagacgacg gatgcatcat ctgcgcgcgc
    10741 catgctatcg gcgtaagact cacagtagaa aaaggcattc tgcgcttttt cagcgaagtg
    10801 atacaactct gcttccggga aatagggttt tttgtagcgg ccaaacggca cgttaaactc
    10861 accgcgcaga ttgtaacgac acagaccgtt gtaaccgtag cggttcaaat ataaaaacag
    10921 taccgcccga cggaacggat cctggctttt gttgaactct tcgcggaact gatagtaaac
    10981 ctcggcgcaa tttgtttcgg gaacaaacag ctcgcgtgcg gcctgtacgt actcatcagt
    11041 acgcatcttc acaatgttat agagactgat caggtcgcta ttgatatcgg caaggatata
    11101 acgagaaaag tcggtgttga gaaacaccga cccggcacct acaaaaggct caaccagaca
    11161 ttcgcccttg ggcaaatgcc gtttaatatc atcaagcagg ggatacttgc cccctgccca
    11221 cttcaaaaaa gcgcgatttt tcttcatgct gactaactaa ttacaccttc tccggctgtg
    11281 gagaaagctc cgacagcatc cagcgcttca ggcaactccc gcgatgataa accattgcgg
    11341 gagataacct tgattacttc agatcggcct gtacctgacg cagcggtttc gcccacgggt
    11401 ttttggcctg gacatcggct ggcaatgtag ataccgcttt tttcgcctct tctttcgaag
    11461 cgtacacgcc agaaaccagg acataccacg gctgaccatt acgcgtcgtt tcatagacaa
    11521 cgtagttttt cagattctct ttcttcgccc aaccgttcag gttgtcgtag ttagaggaac
    11581 tgctcagctg cagagtgtaa tggctggacg gtgccgattt caacgaacca acattacctg
    11641 cggtcttccc tccagcggct ggtgttgccg tggtttgcgc cggggatgcc gtctgtactg
    11701 gagccgtagt cgccgtctct tttggtgctg gcgtagaagt cgcagccggt gccttcgtgc
    11761 ttgccacagg agcagctggt tcagtacgct tcggcgtctg tgctaccggc ttcggctccg
    11821 ttttcacggt tgcttgcggt tttttcggtt caatcaccgc ctgctggcga gctggacgcg
    11881 tagtggccgg acgttcagcg gtttgcgttt tcgccgtgtc acgcgatgca ttgccattgc
    11941 gaacaggcgc gaccgtcgcg ggttcagtcg gcaacgtgga attgaccgcc acattattca
    12001 actgttgctg attttgtggc tgggtcagcg cattgttcag gtcaccctgc acttcaacac
    12061 gttgttgacc atccgttgcc gccggggttt gcccttgagt cggcgtagaa gagatcggcg
    12121 gcagagaaac atcctgctga gtattttccg cagacgtggt tcccggcgct ggctgcacgc
    12181 cattcgcctg atcggtcgca ttgccagcaa gatcaatact cttctcgcca gacgcggttt
    12241 gatcgctgga agaggtcgag ggggctttta gcgcagaacc gataccgatg atcaacagca
    12301 ggagaaccag aatgccgacg cccatcatca tatactgacg agaagcgggt ttactggctg
    12361 cttttttgcg cttacgtgga cgacgctcta cgcgctcttc atccacggtt tcatcttcgg
    12421 attcgtcaat ttcttcttcg agttccggtt cctcattgcg ctctttttgc gcacgagtcg
    12481 gacggcgatc gtcagtgtca tcaagttcaa tatcatcaaa attgatctgc ggttcgccac
    12541 gttcagtacg ctcagaacgt tcagaagatt gacgagaacg accagtacga cgatcgctgg
    12601 gatcgggttt cagctcgtct tctggtttga attcatccat ttaacacccc actaaaaggt
    12661 taatgcttac cacgttgcaa ttaacctgaa gctaatagac cgcttgataa gcggcctgac
    12721 ctttcttgtt gttacgctga ttgacaatcg gcaatggcgt taagaacaag ctcgtgcgaa
    12781 acgccgctgc gaacttcact cttaccaatt gccaacggaa gaattaagcg catctctccc
    12841 gcaaggactt tcttgtcacg cagcatatgc ggtaaatacg cctgcgcaga catttcgcgc
    12901 ggcccattga ccggtaaccc agcccgcgtg agcagggtta taatacgctg cgtttcggca
    12961 gaactaaact gcccgagacg ttccgacgtc cgcgccgcca tcaccatacc cgcagcaacc
    13021 gcttcaccgt gtaaccaatt gccatacccc atttcagctt caatggcatg accaaaggtg
    13081 tgtcccagat tcagtaaagc acgtaacccg gtttcgcgct cgtcggcggc gacaacttct
    13141 gccttcagtt cacaacaacg gcgaatacag tacgccattg ccggaccgtc cagacgcaac
    13201 aacgcatcca gattctcttc cagccagttg aaaaacgcac cgtcaagaat aatgccgtat
    13261 ttgatgactt ctgccagccc cgacgctaac tcacgcgggg gaagcgtttt cagacagtcg
    13321 agatccacca ccactgaagc aggctggtag aacgcgccaa tcatgttttt accgagggga
    13381 tggttgaccg cagttttgcc gccaacggag gaatcgacct gcgacagcaa tgtcgtcggg
    13441 acttgaatga aacgtacacc gcgctgataa ctcgccgccg caaagccggt cagatcgccc
    13501 actacgccgc cgccaagcgc caccagcgta gtatcgcgac catgcggctt ttgtaacaac
    13561 gccgtaaaga cggtatcgag tacagccagg cttttatact gctcgccgtc agggaggata
    13621 acgctatcga cgttaacacc cgcctgttca agtacgccgc ggaccttatc gagatacagg
    13681 ggagccaggg tttcgttggt aaccaacatg acctgctcgc ccgatttcag cggtaagaat
    13741 gaagctggtt cattaaacaa accagatgcg atggtaattg ggtaactacg ttccccgaga
    13801 gtaacgacaa tcctctccat aacgcgacat ccaccttaat tactgtaccc gcagacgagt
    13861 gtatataaag ccagaattag ttgctttcca gcatgtgaat aatctggttt gcaaccactt
    13921 tagcgctttg atcatcagta cgaatggtca cgtcggcaat ctcttcatac agcggattgc
    13981 gttcattggc caacgcttcc agaacttcac gcggcggtgt ttcaacgtgc agcaacgggc
    14041 gttttttatc acgctgcgtg cgtgcaagtt gcttttcgat ggtcgtttca agataaacga
    14101 caacgccacg agcggaaaga cggttacgcg tttcacggga tttcacagag ccgccgccag
    14161 tagccagcac aataccctgt ttctcggtca actcattgat gaccttttct tcgcgatcgc
    14221 ggaagccttc ttcgccttct aaatcgaaaa cccagcccac atcagctccg gttcgtttct
    14281 caatctcttg atcggaatcg taaaattcca tattgagttg ttgagctaac tggcgcccaa
    14341 tagtgctttt tccggcaccc ataggcccaa ccagaaagat attgcgtttc tctgccattt
    14401 tttcggtact actaagacta ttcgttaatg ataaacccgc ttcgctcaga gagcgccgca
    14461 ggacatgaac tgaaacctca taagatattg cgagagtcag actgaaaatt atctcaatac
    14521 tccagcgggt ttggcaactg aataaatcac ccatccctct gcatatctgg tcgctgcaag
    14581 cgcgctgcct tgctaccacc gctctggcga ttaatcaccg ggtaagatta gcgtaaaaaa
    14641 gacagcaaaa tgccgcctga atgataaatc atcatcatgg aacgcaggca gcgaagagca
    14701 ccaactctca aatcggtact ccttgtatgc taaatacctg cgcgcgtcaa atagatgaaa
    14761 cacgttcagc gtaaaaacat taccgctttt acggctgttt actcactgga aaccagtcgt
    14821 ggcgtgataa acaccactaa ctcgcgtcgt tcatcttctt ttccgtcatg acgaaataat
    14881 tgcccgaacc agggaatgtc gccaagcaac ggtacgctat cctgacccga tttattttta
    14941 cgggtaaaaa tgccgcccag cgccaacgtt tctccgcttt tgacctcgac ctgcgtttcg
    15001 atctcctgct tatcaatcgc caacacttcg ccatcggcct gctgtagcac ctgccccgga
    15061 acgttctggc tgatgtgtaa tttcagccgg atgcgacctt tttgtaacac cgtgggcgta
    15121 acttccatcc ccaggacggc ctctttaaat tccaccgacg tcgcgccact ttccccgctg
    15181 gaaacctgat atggaatttc gctcccctgt ttaatgctgg caggctgaag atgtgaggcc
    15241 agcagacgcg gactggcgat aatatccagc tgctgttttt gttcgagcgc ggaaagctca
    15301 agatccagta aacgtccgtt gatgcgtcca atgttaaaac cgacatgcgt tgtcgccgtc
    15361 gctacggaga ggtcgctgcc aagcgtggtg acttgcccaa cgccaccagc ttgctgcgca
    15421 tcagccaacg tccatttcac gcctaactca cgcaaacttt tttcattaat ggtgacaata
    15481 tgcgccgaca gctcaacctg cccgaccggc agatccattt gcgctaccca ctgttcaagc
    15541 gcgcttaacg ccgttttgtt atctcgcagc aaaaggcgat tggtgcgttt atcgacggtc
    15601 atactccctt tggcactcag tagcttctcc ccggctttcg ccagttctcc cgcgtcggcg
    15661 tattgcaggg ttatactgcg attttccagc ggcagatttg cctgcgcccg cgcctgctcc
    15721 gcctcctgac gggcgatatt gtcattctgc caggcaacgg aatgcaccga gagaatgttg
    15781 ccctcctggc gcgttatcaa tccggcgctt ttcactacag tttgtagtgc ctgcttccag
    15841 ggaacgtcag ttaaatgtaa cgacaccgta ccgctgacgt ctggcgaaac caccaggttc
    15901 aacttctcct gttcagccag cgcctgcaac acctgagcta ccggaacgtc atccaccatc
    15961 agcgtcactt tttgcggctt tgccgcctgt acgccgggta taagcatcaa cagtagtgcg
    16021 gctatccatt gcttcatttg tatctccttg ccgttgccac aaccagtgtg gcggttcgca
    16081 gtttgtcccg gttcccagcg ttagtgcgtc tggcgtcagc tgtaaaattg tccagccgtt
    16141 ttccagcacg tcgttttgct gcacccgtcg ccattttttt tgcccgtctt ttattacacc
    16201 gatgatgcgc tcacctcgcc ctaccatccc ctgatagcgc cactggctaa gttcgctaat
    16261 ccggcataga tcttccggcg gtttaaaagg gtcacgcata ccggttaaaa ggcacaatgt
    16321 aatacctgcc aacaaccagc gtttaaccct catgcggcgt ctccagttgt agcgtgaaca
    16381 aaaggtcatc accttccacg cttaacgaaa aacggctcac gctgacgttg cgctctgcca
    16441 gccgtgtaaa tgccgacggc actgcttccc acagcgtttt caacgccaac tcacctccct
    16501 gcgcggatgg atgccagtaa accagttgcg cgccggataa ctgaaaatcc agtggcgaaa
    16561 agggcagcgt tttttcctcg ctgaagggag tggtgtcaac caggcgatac agtgcggccc
    16621 actgctggtg atgacttgcc cgtagtcgaa ttaatgcgtc gctctcttca tggtgtgtcg
    16681 atgacagaaa aatgagcgta actaacatca gcaaccagac tgcccagcaa agctggcgga
    16741 ggcggggtga tgtggcgaac caccagtcaa agagcatgtc catcgctaac cttccttgtt
    16801 aactgatact caaattgcca gcgtccctgc gcatcctgct gcgtggcccc ccgctgattg
    16861 agatgaaaag atgcatcctg gcggagtgac gtttccagcg cgtttaacgc ggtaatgctt
    16921 gttgtcagcc ccttgatctc cagcgtacct tgctgccagc ttatcgttgt cagccaggca
    16981 tgttcgggta aaagcgccgc cagtgcttcc agcgcagatt gccagtcacg ggtaaattgt
    17041 cgctggagct gcctttgcaa acgctgttcg cgtaactgct ggcgttccag caaacgtggt
    17101 ttcgttatct gtaagctgcg ggcgagttgt tgttccgcct gaagcaaaac ggtgtctacg
    17161 cgtgcttcgg cgttgcttgt cagacgcagt attagcgtta tcccgacagc cagcagcaga
    17221 ggcgcaacga acatcagcaa ccagaaacgc agaaaagcgg tccggcgttg ctgtcgccag
    17281 ggcaaaaaat taattggcgg gttcatcagt acgccttccc tagcgccagc cccagcgcga
    17341 tggcaaagtc tccacctggc ggcggtagcg gcggctgacg aacagaaacc gcctcccagg
    17401 gatcaaatcc gccttcgcca catatcgcga cgctttcggg atcaacggat aacgctgccg
    17461 ccagctcctt cgcgctagtc atccccaccg ccagtttgcg cccccagcga tagcgcgtcg
    17521 cccacagcca ctgttcgtta tcacgccagg ccagacattg ctgatgagaa ggtaaaaaag
    17581 gcaggaatcg ctgtaatgca ctggcatccg gggtgatcgc gctaacatga acacgcaacc
    17641 tttctgccag agtaagcagc gttgccaact ctttgctttg cgcggcagtc acgttatagg
    17701 cgggactgag tgagtcttcg ctgtaatcga agcgcaggga gtccggatcc atatccagct
    17761 cgcgggccat cgtcccggac agccaggccg tttgctcccg ctcaccaagg gacatcgacg
    17821 gacgtggaaa tgaccgctgt aatgtgcgac tggcgggaaa cgccaacata atgtgatgac
    17881 gctgcggcag ttcgcgactc cacggtaaca acgttttagc cagccgctgc acatcaacaa
    17941 tccgcccatc tttgataatg tcgttctcca gcggcaaccg ccaccagcgt tgcaaaaagc
    18001 attcttttgt gccccaaccg ctaccgcttc ttgctgttgt aaatgcaaac caatttgcca
    18061 gatcttaaat gccattgtga tgatctcctt atcacccgtc actctgacgg gtatatcaat
    18121 gcgtctggct tgcctttata ctaccgcgcg tttgtttata aactgcccaa atgaaactaa
    18181 atgggaaatt tccagtgaag ttcgtaaagt attttttgat ccttgcagtc tgttgcattc
    18241 tgctgggagc aggctcgatt tatggcctat accgctacat cgagccacaa ctgccggatg
    18301 tggcgacatt aaaagatgtt cgcctgcaaa ttccgatgca gatttacagc gccgatggcg
    18361 agctgattgc tcaatacggt gagaaacgtc gtattccggt tacgttggat caaatcccgc
    18421 cggagatggt gaaagccttt atcgcgacgg aagacagccg cttctacgag catcacggcg
    18481 ttgacccggt ggggatcttc cgtgcggcaa gtgtggcgct gttctccggc cacgcgtcac
    18541 aaggggcgag taccattacc cagcagctgg cgagaaactt cttcctcagt ccagaacgca
    18601 cgctgatgcg taagattaag gaagtcttcc tcgcgattcg cattgaacag ttgctgacga
    18661 aagacgagat cctcgagctt tatctgaaca agatttacct cggttaccgc gcctatggtg
    18721 tcggtgctgc ggcacaagtc tatttcggta aaacggtcga ccaactgacg ctgaacgaaa
    18781 tggcggtgat agccgggctg ccgaaagcgc cttccacctt taatccgctc tactcaatgg
    18841 atcgtgccgt cgcgcggcgt aacgtcgtac tgtcgcggat gctggatgaa gggtatatca
    18901 cccaacaaca gttcgatcag acacgcactg aggcgattaa cgctaactat cacgcgccgg
    18961 agattgcttt ctctgcgccg tacctgagcg aaatggtgcg ccaggagatg tataaccgtt
    19021 atggcgaaag tgcctatgaa gacggttatc gcatttacac caccatcacc cgcaaagtgc
    19081 agcaggccgc acagcaggcg gtacgtaata acgtgctgga ctacgacatg cgccacggct
    19141 atcgcggccc ggcaaatgtg ctgtggaaag tgggcgagtc ggcgtgggat aacaacaaga
    19201 ttaccgatac gctgaaggcg ctgccgacct atggtccgct gctgcctgcc gcagtcacca
    19261 gcgccaatcc tcaggaagcg acggcgatgc tggcggacgg gtcgaccgtc gcattgagta
    19321 tggaaggcgt tcgctgggcg cgtccttacc gttcggatac tcagcaggga ccgacgccgc
    19381 gtaaagtgac cgatgttctg caaacgggtc agcaaatctg ggttcgtcag gttggcgatg
    19441 catggtggct ggcgcaagtg ccggaagtga actcggcgct ggtgtcgatc aatccgcaaa
    19501 acggtgccgt tatggcgctg gtcggtggct ttgatttcaa tcagagcaag tttaaccgcg
    19561 ccacccaggc actgcgtcag gtaggttcca acatcaaacc gttcctctac accgcggcga
    19621 tggataaagg tctgacgctg gcaagtatgt tgaacgatgt gccaatttcc cgctgggatg
    19681 caggtgccgg ttctgactgg cagccgaaga actcaccacc gcagtatgcc ggtccaattc
    19741 gcttacgtca gggactgggc cagtcgaaaa acgtggtgat ggtacgcgca atgcgggcga
    19801 tgggcgtcga ctacgctgca gaatatctgc aacgcttcgg cttcccggcg caaaacattg
    19861 tccacaccga atcgctggcg ctgggttcag cgtcattcac cccaatgcag gtggcgcgcg
    19921 gctacgcggt catggcgaac ggcggcttcc tggtggaccc gtggtttatc agcaaaattg
    19981 aaaacgatca gggcggcgtg attttcgaag cgaaaccgaa agtagcctgc ccggaatgcg
    20041 atattccggt gatttacggt gatacgcaga aatcgaacgt gctggaaaat aacgatgttg
    20101 aagatgtcgc tatctcccgc gagcagcaga atgtttctgt accaatgccg cagctggagc
    20161 aggcaaatca ggcgttagtg gcgaagaccg gcgcgcagga atacgcaccg cacgtcatca
    20221 acactccgct ggcattcctg attaagagtg ccttgaacac caatattttt ggtgaacccg
    20281 gctggcaggg tactggctgg cgagcaggtc gtgatttaca acggcatgat ataggcggaa
    20341 aaacgggtac cactaacagt tcgaaagatg cgtggttctc gggttacggt ccgggcgttg
    20401 tgacctcggt ctggattggc tttgatgatc accgtcgtaa tctcggtcat acaacagctt
    20461 ccggagcgat taaagatcag atctcaggtt acgaaggcgg tgccaagagt gcccaacctg
    20521 catgggacgc ttatatgaaa gccgttcttg aaggtgtgcc ggagcagccg ctgacgccgc
    20581 cgccgggtat tgtgacggtg aatatcgatc gcagcaccgg gcagttagct aatggtggca
    20641 acagccgcga agagtatttc atcgaaggta cgcagccgac acaacaggcg gtgcacgagg
    20701 tgggaacgac cattatcgat aatggcgagg cacaggaatt gttctgatta aaaaggcgct
    20761 tcagcgcctt tttaagtttg ctgacaaagt gcactttgtt tataccggat acggcgtgaa
    20821 cactttaccc gtctaaaaac atctagaaaa atcaatatat tgcaggagcc acacaggcct
    20881 gataagcatt atgcatcagg caaacttcac gcatttacaa ccgcccctgc cccttcaacc
    20941 attcgcgcac gaggaaaagc gcgctgacat tacgcgcttc gttgaagtca gggtcttcca
    21001 gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct ggctcatcgc
    21061 cttccagcga ttccgggtag agatcttgcg ctaccacgat attcattttg ctggaaaagt
    21121 aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca aatcccacct
    21181 cttcttttag ctcgcggttc gcggcttcga agacgctttc acccggatca attaatcctt
    21241 tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc aggatcaggt
    21301 gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc attcgttcat
    21361 aaacacgccg cacgccattg ctgaactcca gatccacgct ttcgacggta aacagtcggg
    21421 aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg ctcatcgtgg
    21481 gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc ggcaatgtga
    21541 attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat caaattaaaa
    21601 gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga gggtttgcta
    21661 tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac gcttgccgat
    21721 agccaaccgc agaatcatgt tttgtgtccg gtgcgactga ccacgcctga cagactaagt
    21781 aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg gcctgctcac
    21841 tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc tggaccaacg
    21901 ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc gttgaaaatt
    21961 atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagcc agcgcggcac
    22021 cgatttctct ggcgctgaat gccgaaagca acaacgtcat gatgctaaca cacgctatca
    22081 cgcgttacgg catctctacc gacgatccga ataaatggcg ttactacctc gattcggtag
    22141 aagtccacct gccccctttc tgggagcagt acatcaacga tgagaatacc gttgaactga
    22201 ttcataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg caggagtaca
    22261 tgcaggaaac tcgcggctat gccttgcaac ctgttccgtc aacacaggcg tcgattcgcg
    22321 gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcac gaagagtatg
    22381 cgctaagtcg tccgcgcggg ctgcgcgaag cgttgctgat tgtcgcctcc ttcctgatgt
    22441 tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc ggcgcgttac
    22501 tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc tccctgcggg
    22561 aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa aacgatcagg
    22621 aacagatcaa caatatttca ctcggtatta tcgacctggt ctaccccgca cactggcagc
    22681 cctacattgc tcatgatctc ggccaacaaa ccgatatcga tatctatctc gaccgccacg
    22741 tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt ccgttacagc
    22801 actggctgcg cagtacgatt atcgctgcgg gttcgctgct ggtgctgttt atgctgttat
    22861 tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa ggcgcgcaga
    22921 ccattgaagc caccagcgtg aaacaactgg ctgacgctgg cgtacgggtt ggcgatacat
    22981 tgcgtattag cggtacggga atgtgtaata ttcgcacttc cggtacctgg agcgcgaaaa
    23041 ccaactcacc ttttttaccg tttgactgct cgcagatcat ctggaatgac gcccgctcat
    23101 tgccattacc agaatctgaa ttggtcaaca aagcgacggc attgaccgaa gcagttaatc
    23161 gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag cgcctcatta cgttcagcaa
    23221 ttcaaaaatc cggcatggta ttgcttgatg attttggcga catagtactg aagacagcgg
    23281 atctgtgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc aatctcggca
    23341 acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaaactc gatggcgtga
    23401 atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca acctccaccg
    23461 cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt ccggcccccg
    23521 gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc tggccttcgg
    23581 catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa ctggcacaaa
    23641 tgctgatgca tacgccgttt aacgccgagg gtatcgtcac aaaaatcttc actgacgcca
    23701 atggtacgca gcatataggc cttcatccga tcccggatcg ttccggcctg tggcgctatc
    23761 tcagcaccac gctgctgcta ctgacgatgc tgggtagcgc catttataat ggcgtacagg
    23821 cctggcggcg ttaccagcgt catcgtactc gcatgatgaa gattcaggcc tattatgaaa
    23881 gctgcctgaa cccacaactg atcacccctt cagaaagcct tatcgaataa cacgtttgcg
    23941 cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc cctcatgcat
    24001 atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg cacgttgctc
    24061 gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg gggcgcgaaa
    24121 aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca cgacgtacag
    24181 catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct ggatatctgt
    24241 gcgatgacca ccgagatggg accgcgcgcc gtactgcgtg aagataccat tccgtttctt
    24301 gaggcactga aagccagcgg taagcagcga attttgctca ccaatgcgca tccgcacaac
    24361 ctggcggtaa aacttgagca taccggtctg gacgcacacc ttgatttatt actttccacc
    24421 cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc cgaagctacg
    24481 ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct cgatgctgcc
    24541 gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgactccgg gattgccgag
    24601 aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc ctcgctaatg
    24661 tgaaggagac gccatgaaag aaaaacctgc tgttgaggtt cgactggata aatggctatg
    24721 ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag gcggtaaggt
    24781 gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg ccacgctcac
    24841 tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg aacagcgtcg
    24901 ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag agaaacgcga
    24961 aaaaatggcg ctggcacgta aacttaatgc cttaaccatg ccgcacccgg accgacgccc
    25021 ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg aataactgtc
    25081 acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc tgtttgaaaa
    25141 ctttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga tccttgagaa
    25201 ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg cgaccagcct
    25261 gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcaag gcgacggtcc
    25321 gatgaatctg gcggttatta acggtaacaa caaccagcag atgcgcggtg tggcgcgcgt
    25381 gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg gttacgtggt
    25441 gatcaccatc accccgagcg aaggtgaacg ctatcagggc gtggtgggtc tggaaggtga
    25501 tactctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc cgacgcgcct
    25561 gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt tgttgcaggt
    25621 aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcgacgc taaccgaaac
    25681 catcaaaacc gaagaactgt tgaccttacc ggcaaacgaa gtgttgtggc gtttgtatca
    25741 cgaagaagag gtgacggttt atgatccgca ggatgtggag ttcaaatgca cctgctcgcg
    25801 tgaacgttgc gccgacgcgc taaaaacgct gccggatgaa gaagtcgata gcatcctggc
    25861 ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc tgttcaatgc
    25921 gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag ttcattaatg
    25981 agtatgtccg gcagagaggg ctctgccgga ttttacgacg taatggcctg atgcgcttat
    26041 cagacctacg gattcaatgc aatttatcgc aggtaagata atgtgccgaa tccaataaaa
    26101 atcagatatg cgctgtcatt cattcagatt caaatccagt ctttcgccat ccaccgtaag
    26161 atcgcgccac actttaggct tcgtccccag ttttccatcc tccgcagcca tgagtaattt
    26221 ttctttcgtt attacagccg ggagtgaatt catcgtaatc tctttccatt ctttcccagc
    26281 aggatcaata ccatagataa tgtatctttc atcattaaag gcaaacagga tccgctctgc
    26341 cctgccatca ctattcagat cttgctctac cagcacgcag gcatcttttt caatgcatat
    26401 cattgcgtta tagcgatttt tcatcactgc cgaccagaat atcgtatccg gttttccaga
    26461 acctgaggca ataaatacat tatctgctaa tgctttttcc gatatctgct gctgaagacg
    26521 ttgtactcca tcaaatgcca tcagcagacc ccgcctgcgt ttcgggtctt tcatatactc
    26581 ggcatcgctt ttcagcgact caagcgccgc acgtccatag cgaccgctct gttcgagcat
    26641 atagagactt acctggtctg gcgtgttttt gccgctctga taacgcgcca tatgactgtt
    26701 cacgctaatg cgcatactgt ccagcaccgg agaattaaga agcaccagta ttaccaacac
    26761 caataacgaa accgcaaggt tcactttgcc ctgaagaaca agggggtttt gccctttacg
    26821 ccagacgata ctgacaaaat accccagcga ccataccagt aacaccagca ccgccagcgc
    26881 gccctgcaag cggtcgacag tccagccgta ctgagcgacc cgcagccata acgcccaggc
    26941 ggcgacgaac acatacagcg gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg
    27001 ccccgtccag ggaagagacg ctttttgcgg atcgcggaca atagccatta agatcaattg
    27061 caaaaaggcc agcgttaaca gcaacccagc ggcggagatg tggcgagaaa tcgcgctcag
    27121 gcccgtaaac ggcagggtga taataaacat cagggttagc aatgatacta acggcagcaa
    27181 ccccgtggcg attaacgtga acaacttttg aatagagtcg attaaacgtg actgtgtacg
    27241 cgcgaggatc accgccagtg cggtaaccag acctaacgtg agataaataa accagtcggt
    27301 tgcaaaaaag agcgttttga aaaacgtgat gccaacaagt ttaaacaact ctccccacag
    27361 taaaagtacc agccacgtca ggccattggc gagaaaaatc accagtaata taagtacgtt
    27421 atgccatact gactggtaaa aatagcgata gcggctactg tcattgcgga tgcgaaggct
    27481 ttgttgtatc cacggtagca acaacattgc catcagcagc agatagcaac caaaatccca
    27541 aagcgccttt tctgctctcc aggggttcat gccatcaatt tgccacttta gccaaccgct
    27601 catccctagc gtggcaataa acaccagcgc cagccatccc caaaggcgct tctgtttaaa
    27661 ggagatcacg gagaagagta aaacggatga aaacgcgacg gtcgcaggca cgccataaac
    27721 aatccaactg tgattttttc cggcaagcca ggcgatcagc aggtagcaaa ccagaccctg
    27781 aagtaatccg gtagcaatca tcccccagcg tgttgccggt gaaagttcaa cgttatccat
    27841 gttagttatc cagaatcaaa aagtgggtta attattgcat ccgggccgta gtattttgct
    27901 tttttcagaa aataatcaaa aaaagttagc gtggtgaatc gatactttac cgattgaatt
    27961 tgcatcaatt tcattcagga atgcgattcc actcacaata ttcccgccat ataaaccaag
    28021 atttaacctt ttaagaacat tttccacacc taaaatgcta tttctgcgat aatagcaacc
    28081 gtttcgtgac aggaatcacg gagttttttg tcaaatatga atttctccag atacgtaaat
    28141 ctatgagcct tgtcgcggtt aacaccccca aaaagacttt actattcagg caatacatat
    28201 tggctaagga gcagtgaaat gcgcgttaac aatggtttga ccccgcaaga actcgaggct
    28261 tatggtatca gtgacgtaca tgatatcgtt tacaacccaa gctacgacct gctgtatcag
    28321 gaagagctcg atccgagcct gacaggttat gagcgcgggg tgttaactaa tctgggtgcc
    28381 gttgccgtcg ataccgggat cttcaccggt cgttcaccaa aagataagta tatcgtccgt
    28441 gacgatacca ctcgcgatac tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac
    28501 aaacctctct ctccggaaac ctggcagcat ctgaaaggcc tggtgaccaa acagctttcc
    28561 ggcaaacgtc tgttcgttgt cgacgccttc tgtggtgcga acccggatac tcgtctttcc
    28621 gtccgtttca tcaccgaagt ggcctggcag gcgcattttg tcaaaaacat gtttattcgc
    28681 ccgagcgatg aagagctggc aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag
    28741 tgcactaacc cgcagtggaa agaacagggt ctgaactccg aaaacttcgt ggcgtttaac
    28801 ctgaccgagc gcatgcagct gattggcggc acctggtacg gcggcgaaat gaagaaaggg
    28861 atgttctcaa tgatgaacta cctgctgccg ctgaaaggta tcgcttctat gcactgctcc
    28921 gccaacgttg gcgagaaagg cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa
    28981 accacccttt ccaccgaccc gaaacgtcgc ctgattggcg atgacgagca cggctgggac
    29041 gatgacggtg tgtttaactt tgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa
    29101 gaagcggaac ctgaaatcta caacgctatc cgtcgtgatg cgctgctgga aaacgtcacc
    29161 gtgcgtgaag atggcactat cgactttgat gatggttcaa aaaccgagaa cacccgcgtt
    29221 tcttatccga tctatcacat cgaaaacatc gttaagccgg tgtccaaagc gggccacgca
    29281 actaaagtta tcttcctgac cgcagacgcc tttggtgtgc tgccgccggt ttctcgtctg
    29341 actgccgatc aaacccagta ccacttcctc tctggcttca ccgccaaact ggccggtaca
    29401 gagcgcggca tcaccgaacc gacgccaacc ttctccgcct gcttcggcgc ggcattcctg
    29461 tcgctgcacc cgacgcagta cgcagaagtg ctggtgaaac gtatgcaggc ggcaggcgcg
    29521 caggcgtatc tggttaacac cggctggaac ggcactggca aacgtatctc cattaaagat
    29581 acccgcgcca ttatcgacgc catcctcaac ggttcgctgg ataacgcaga aaccttcact
    29641 ctgccgatgt ttaacctggc gatcccaacc gaactgccgg gcgtagacac caggatcctc
    29701 gatccgcgta acacctacgc ctctccggaa cagtggcagg aaaaagctga aacgctggcg
    29761 aaactgttta tcgacaactt cgataaatac accgacaccc ctgcgggtgc cgcgctggta
    29821 gcggcaggtc ctaagctgta atcagctgat atctcattga ccccttatcc aaacggataa
    29881 ggggtttttt tatctaatta gctaccaata tgtacacggt acacttataa ttatcaatag
    29941 accgcaggat gcattatgtt cacctttatt gaactacaag gatttagcaa acgccgccag
    30001 ttattgctac ctgatgatga gttccgggct tttcaggaac tactcattca agatcccact
    30061 gccggtgaca ttatcgcagg cacaggcagt tttagaaaaa ttcgctggaa ccgttctgga
    30121 atggggaaac ggggtggcat tcggataatt tattacaacg taacacgcaa tggccgaatt
    30181 tatctggcgt tgatttatcc taaaaatgaa caggatgatt taacagaaga acaaagaaag
    30241 gcattgaaac tattgtccga gaagctgctt taatgttgaa gctccacaaa ttccaccatg
    30301 gccgaaatgt caacggaggc attgaaaaca gatgaaagat gaattatttg ctgatcttct
    30361 tgccagcgca gaagaaatgg tacgcatcga aaagggtgaa gaaacgcctg aaccagagca
    30421 cgtccatact tttagcgaaa tcgatgtaaa agcgatccgt gaagcaacgg gtttacgtca
    30481 gcaagatttt gctgtcgccg taggggtaag ttatgacctg gtcaaaagct gggaaaccaa
    30541 acgccgtcaa cctaccggag caccccgaaa attgctcctg cttttgcaag cgaatccttt
    30601 cattatcaat caattgaaaa caatctaaaa acaggaggca ccttcgcctc ccgttttatt
    30661 tacccttctt ttgtcgtgcc ctgcgcccgc gttaccggca ctggcagcca ggcgcgaatg
    30721 gaaagcccgc cccgctcgct gctgccaagc tccagcatgc cgttatggtt atccacgata
    30781 cgctgcacaa tcgccagccc taatcccgtg ccgctaatgg tgcgcgcact gtcgccgcga
    30841 acaaacggct ggaacaggtg cttacgttgt tccggcgcaa ttcccggacc atcatcttcc
    30901 acctggaacc aggcgcgatt cggctccgtt ccgctgctga ctttgatcca gccattgcca
    30961 tagcgggcag cgttcaccac catattcgcc accgcacgtt tgatcgacag cgggtgcatt
    31021 ttcacctcaa tgctgccggg gtaaagcgcg gtttcaattt cccgctcata gccactttcg
    31081 gcggcaatca cctcaccgag cactgcgttg agatccgcca tttccatcgg catctcctgc
    31141 ccggtgcgca ggtagtcgat aaactgctca atgatggcgt tgcactcttc gatatcttta
    31201 ttgatcgatt cggccaggta gccatcctgc tcgctcatca tctcggtcgc caggcgaatg
    31261 cgcgtcagcg gcgtacgcag atcatgactt acccccgcca tcagcagcgt gcgatcatcc
    31321 gccagttgct taacaccagc cgccatatgg ttaaaggcgc gggtaacgga acgcacctcc
    31381 gaagcgccat attcacgcag cggcggcgga ataatccctt taccgacctg cagagcagca
    31441 tgttcgagat cgaccaacgg tcggttctgg atacggataa acagccacgc cccgcctatc
    31501 gccaatagca taatcgccag cgtatagcgg aacagcggag aaaaatcacc ctgatgaatt
    31561 tcggtcagcg gcacgcgcac ccagatattg ggcgacagcc aggttttcag ccagacaaca
    31621 ggcgaacttt tgttgacctc aacgcgcact tccgtcgggc cgcccagttg ctgcgccatc
    31681 tgatggctta agaattcata gtgttgtgcc caacgcagac ctgcctcttc ggcagcttcg
    31741 ttggaataga gagagatccc cagctcacgg tagatctccc gacggaaagc gggaggcaca
    31801 accaactgcg tgccgtcctc cagttgcagt ttgtcggtca tcaacatacg cacttcgtac
    31861 gcgaggactt tattaaactg ctggaggctc ggcaaaatcg cgaagttcag caccaccaga
    31921 taagtcgtca ccaggctgac gaacagcaag gtgacgatga gcaataacgt acgggcaaat
    31981 gaacttcgtg gcgagaagcg caatcgcctc atgctttaga accgtccggt acaaagacgt
    32041 agcccagacc ccagacggtc tgaatgtaac gcggatgcgc cggatcttct tccaccatgc
    32101 ggcgtagacg cgaaatctgc acgtcgatgg agcgttccat tgcggagtat tcacggccac
    32161 gggcaaggtt catcagctta tcgcgggaga gcggctcacg cggatggctg accagtgcct
    32221 tcagtaccgc aaactcaccg ctggtgagcg gcatcggctc atcttcgcgg aacatttcac
    32281 gcgtaccgag gttaagtttg aacttaccga aagcaattac cgcctcttcc tgtgacggcg
    32341 cgcccggcag ttcgttggcc tgacgacgca gcaccgcacg aatgcgggcc agcaattcac
    32401 gcgggttaaa cggttttgga atgtagtcgt cagcgccaat ctccaggcca acgatacggt
    32461 ccacttcttc ccctttcgcc gtcaccataa tgatcggcat cgggttgctc tggctacgaa
    32521 gacgtcggca aatcgacaag ccatcttcac caggtaacat caaatccagt accataagat
    32581 ggaaagattc acgagtcagc aggcgatcca tctgttctgc attagcgacg cttcgaacct
    32641 ggaagccttg ttcggtgaga taacgttcca gcagcgcgcg caggcgcata tcgtcatcga
    32701 ccaccagaat cttgtagttc tcttgcattg tttgtactcc caaaggttcg caacaatttg
    32761 taagcgtgta ttcttaaaaa agctcacgtt cgtcaccagc taaatctggt atgaatttca
    32821 gcctaaattg ttacaaagca tattaaacag cagcttaagt atacaattta ttcggcgaaa
    32881 cattattgat tctgttgata tgatcacgtt atacccaatg tgcgcattat caaacagaca
    32941 aagggaatca acgagatgaa aacgcccctg gttacccggg aagggtatga aaaactcaaa
    33001 caagagctta attatctctg gcgtgaagaa cgcccggagg tcacaaaaaa ggtgacctgg
    33061 gccgcaagtc tgggcgaccg cagcgaaaat gctgactatc agtataataa aaagcgcctg
    33121 cgtgaaatcg accgtcgcgt gcgctatctc actaaatgca tggaaaatct caaaatcgtc
    33181 gattactccc ctcagcagga aggcaaagtc ttttttggtg cgtgggtgga gattgaaaac
    33241 gacgatggcg tgactcaccg tttccgtatt gtcggctacg atgaaatttt tggccgtaaa
    33301 gattacatct ctatcgattc cccgatggct cgcgcattgt tgaaaaaaga ggtgggcgat
    33361 ttggcggtgg tgaatacccc tgccggggaa gcgagctggt atgtgaatgc tatcgagtac
    33421 gtgaaaccgt aaggaagagt cttaacctcc tgccgatggc tggcattttt gccagccagt
    33481 ccgtataact atcccctgat ttttgatccg aaaagatgaa ctcaaaccat gatgaatgat
    33541 tcgttctgcc gcattattgc gggtgaaatt caggcgcgcc cggaacaggt tgacgctgcc
    33601 gttcgcctgc ttgacgaagg gaataccgtg ccgtttatcg cacgttatcg taaggaaatc
    33661 accggcggtc tggatgacac gcagctgcgt aatctggaaa cgcgtctgag ctatctgcgc
    33721 gagctggaag agagacgtca ggcgatcctc aagtccattt ccgagcaagg caaactcact
    33781 gacgagctgg cgaatgccat caacgccaca ctaagcaaaa ccgaactcga agacctctac
    33841 ctgccctaca aacctaaacg ccgcacccgc gggcaaatcg ccattgaagc agggcttgag
    33901 ccgttggctg acctgctgtg gagcgatccg tcacacacgc cagaagtcgc cgctgcacaa
    33961 tatattgatg ccgataaagg cgtggcagat accaaagccg cgctggacgg cgcgcgctat
    34021 atcctaatgg aacggtttgc cgaagatgcc gcgctgctgg cgaaagtgcg tgattatctg
    34081 tggaagaacg cgcatttggt ctcaaccgtg gtgaatggta aagaagagga aggggcgaaa
    34141 ttccgcgact atttcgatca tcacgaaccg ctgtccacgg tgccttctca ccgcgcgctg
    34201 gcgatgttcc gtggtcgtaa cgaaggcgta cttcagcttt cgctgaatgc cgatccgcag
    34261 ttcgaagagc cgccgaaaga gagctattgc gagcaaatca tcatggatca ccttggcctg
    34321 cgcctgaaca atgccccggc ggatagctgg cgcaaaggtg tggtgagctg gacgtggcgc
    34381 atcaaggtgt tgatgcatct ggaaactgaa ctgatgggca ccgtgcgcga acgcgcggaa
    34441 gatgaagcaa tcaacgtctt tgcccgtaac ctgcacgatc tgctgatggc ggcccctgcc
    34501 ggactgcgtg caacgatggg cctcgatccg ggtctgcgta ccggggtaaa agtggcggta
    34561 gtcgatgcga caggtaaact ggtggcgacc gataccattt atccgcatac cgggcaggcc
    34621 gcaaaagcag cgatgaccgt tgctgcgctg tgtgaaaagc ataacgttga actggtggcg
    34681 atcggcaacg gtacagcttc tcgcgaaacc gaacgtttct atctcgacgt gcagaagcag
    34741 ttcccgaaag tgaccgcgca gaaagtgatc gtcagcgaag ctggcgcgtc ggtttactcg
    34801 gcttccgagc tggcagcaca ggaattcccg gatctcgacg tttcgctgcg tggcgcggtg
    34861 tctatcgccc gtcgtttgca ggatccgctg gcggagctgg tgaaaatcga tccgaaatct
    34921 atcggcgtag gtcagtatca gcatgacgtc agccagacgc agctggcacg caaactggat
    34981 gcagtagtag aagactgcgt aaacgccgtt ggcgtcgatc tcaacaccgc ttctgttcct
    35041 ctgttaaccc gcgtggcggg cctgacgcgc atgatggcgc aaaacatcgt ggcctggcgc
    35101 gatgagaacg gccagttcca gaaccgtcag caactgttaa aagtgagccg tctggggccg
    35161 aaagccttcg agcagtgcgc gggcttcttg cgcattaacc atggtgataa cccgctggac
    35221 gcgtctaccg tccacccgga agcctatccg gtggtggaac gcattctggc agcaacacag
    35281 caggcactga aagatctgat gggtaacagc agcgagctgc gtaacctgaa agcgtctgac
    35341 tttactgatg aaaaattcgg tgtgccgacg gtaaccgaca tcatcaaaga gctggaaaaa
    35401 ccgggtcgcg atccgcgtcc ggaatttaaa accgctcagt ttgccgatgg cgtcgagaca
    35461 atgaacgacc tgcaaccggg tatgatcctc gaaggtgcgg tgaccaacgt caccaacttt
    35521 ggcgcgtttg tcgatattgg cgtgcatcag gacggcctgg ttcacatctc ttcattgtcg
    35581 aacaagtttg tggaagatcc gcataccgtg gtgaaagcgg gcgacattgt gaaggtgaaa
    35641 gtgctggaag tggatcttca gcgtaaacgt atcgccctga ccatgcgtct ggacgagcag
    35701 cctggcgaaa ccaacgcccg tcgcggtggc ggtaacgatc gcccgcaaaa caaccgcccg
    35761 gcagccaaac cgcgtgggcg tgaagcgcag cctgccggta atagcgcgat gatggatgcg
    35821 ctggcggcgg caatgggcaa aaaacgttaa acgcccggac tggcccctaa acctgcgcga
    35881 ttaggcgttc acgccacatc tggcaacgaa cgccttgcct gacataaaag tgccggagaa
    35941 tatctccggc ctttttattc cacaagcaaa ctcataatat atcccattaa tatttatcac
    36001 ttcattaaca actgaaacct taattaaaca ttaaccagcc ctaataattc gtcatgcgaa
    36061 ttatattttc gctgcgatat aaccttgagc catatcaaca ttgagtcaga ttattattca
    36121 aaccaacatt cacacacatt ttaagtattg ctgatagaaa ccattctcat tatcattgta
    36181 ctgttgatta tttaatctct ccttcgttgg caaatcatct ggtctcatgt cgctgtcaaa
    36241 cgccccatga ggtagttatc cagttaatga gaaacaagta ggcacctatg caatacactc
    36301 cagatactgc gtggaaaatt actggctttt cccgtgaaat cagcccggca tatcgccaaa
    36361 aactgctttc tcttggcatg ttacctggct cctcttttaa tgtggtgcgc gtcgctccac
    36421 tcggcgaccc cattcatatc gaaacccgtc gtgtgagcct ggtattacga aaaaaagatc
    36481 tggccttatt agaagtggaa gcggtttcct gttaatacgg tgataacaac aatgaaaaaa
    36541 ttaaccattg gcttaattgg taatccaaat tctggcaaga caacgttatt taaccagctc
    36601 actggctcac gtcagcgtgt aggtaactgg gctggcgtta ccgtcgaacg taaagaaggg
    36661 caattctcca ccaccgatca tcaggtcacg ctggtggacc tgcccggcac ctattctctg
    36721 accaccatct catcgcagac ctcgctcgat gagcaaatcg cctgtcacta cattttgagt
    36781 ggcgacgcag acctgctgat taacgtggtg gatgcgtcta accttgagcg taacctgtac
    36841 ctgacgctgc aactgttgga actcggcatt ccctgcatcg tggcactgaa catgctcgac
    36901 attgccgaga agcaaaatat tcgtattgaa attgatgctc tgtcggcgcg tcttggctgc
    36961 ccggtgatcc cgctggtttc aacccgtggt cgcggtattg aagcgctcaa gctggcgatt
    37021 gatcgctata aagctaacga gaatgtggaa ctggtgcatt acgcacagcc gctactcaac
    37081 gaagcagatt cactggcaaa agtgatgcct tccgacatcc cgctgaaaca acgtcgctgg
    37141 ctgggcctgc aaatgctgga aggcgatatc tacagccgcg cctacgccgg tgaagcgtcg
    37201 cagcatctgg atgccgccct cgcccgtctg cgtaatgaga tggacgatcc ggcgctgcac
    37261 attgccgatg cgcgttacca gtgcattgct gccatctgtg atgtggtaag caacaccctg
    37321 acggcagaac ccagccgttt caccactgcg gtagataaaa tcgtgctcaa ccgtttcctc
    37381 ggtctgccga ttttcctctt tgtgatgtac ctgatgttcc tgctggctat caacatcggc
    37441 ggggcgttac agccgctgtt tgatgtcggt tccgtggcgc tgtttgtgca tggtattcag
    37501 tggattggct acacgctcca cttcccggac tggctgacta tcttcctcgc ccagggcctg
    37561 ggtggcggta ttaacaccgt gctgccactg gtgccgcaga tcggcatgat gtacctgttc
    37621 ctctccttcc ttgaggactc cggctatatg gcgcgtgcgg cgtttgtgat ggaccgcctg
    37681 atgcaggcgc tgggcttgcc ggggaaatcc tttgtaccgc tgatcgtcgg cttcggttgt
    37741 aacgtaccat cggtaatggg cgcacgtacg cttgatgcac cgcgtgaacg cctgatgacc
    37801 atcatgatgg caccgtttat gtcctgcggc gcacgtctgg ctatcttcgc agtattcgcg
    37861 gctgccttct tcgggcagaa cggtgcgctg gcggtcttct cgctgtatat gctgggtatc
    37921 gtaatggcgg tgctgacggg cctgatgctc aagtacacca tcatgcgcgg tgaagcgacg
    37981 ccgtttgtca tggagctgcc ggtctaccat gtaccacacg ttaaaagcct gattatccag
    38041 acctggcagc gtctgaaagg cttcgttctg cgtgctggta aagtgattat catcgtcagc
    38101 attttcctga gcgccttcaa cagcttctcg ctgagcggaa aaatcgtcga taacatcaac
    38161 gactcggcgc tggcttccgt cagccgggtg atcaccccag tcttcaagcc gattggcgtg
    38221 catgaagata actggcaggc aacggttggc ctgtttacag gcgcaatggc gaaagaagtg
    38281 gtggtgggca cgctcaacac cctctacacc gcagaaaata ttcaggacga agagttcaat
    38341 ccggcagaat ttaacctcgg tgaagagctg ttcagcgcgg tagatgaaac ctggcagagc
    38401 ctgaaagaca ccttcagcct tagcgtactg atgaatccca ttgaagcgag caaaggcgac
    38461 ggcgaaatgg ggaccggggc gatgggcgtg atggatcaga aattcggtag cgcagctgcc
    38521 gcatacagct acctgatttt cgtcctgctg tatgtaccgt gcatctcggt gatgggggct
    38581 atcgcccgtg aatcaagccg tggctggatg ggcttctcca tcctgtgggg gctgaatatc
    38641 gcttactcac tggcaacatt gttctatcag gtcgccagct acagccagca cccaacttac
    38701 agcctggtgt gcattctggc ggttatcctg tttaacatcg tggttatcgg tttgctgcgt
    38761 cgcgcgcgta gccgggtgga tatcgaactg ctggcaacgc gcaagtcggt aagcagttgc
    38821 tgcgcagcca gcaccaccgg tgattgccat taatggcttc acttattcag gtgcgtgatt
    38881 tgctggcgtt acggggccgt atggaagcgg cccagataag ccagacattg aacaccccgc
    38941 agccaatgat taacgccatg ctgcaacagc tggaaagcat gggcaaagcc gtgcggattc
    39001 aggaagaacc tgacggctgc ctctctggca attgtaaaag ctgcccggaa ggcaaagcct
    39061 gcctgcgcga gtggtgggcg ctgcgttaac catactccat cacctgtttt gaaaagcccg
    39121 gtatgcgttt gcatccgggc ttttttgcgt gcggctttcc ataaaaatgc aacccttgca
    39181 gcgcgacgta aattcctctg aaagcgtctc gcagggatga aaactcgcta atgcacaggt
    39241 gtggagtggt gcgtagagtc gcggcattca aacaacaggt gaaggaacgc catgagcaaa
    39301 aagcagagtt ccaccccaca cgatgcgctg ttcaaactct ttttacgcca accggagacg
    39361 gcgcgcgatt ttcttgcgtt tcatttaccg gcacctattc acgcgctctg tgatatgaaa
    39421 accctcaagc tggagtcgag cagctttatt gatgacgatc tgcgtgaaag ctattccgac
    39481 gtgctgtggt cggtgaaaac ggaacaaggg ccaggatata tctattgtct gattgaacat
    39541 caaagcacct caaacaaact gatcgcattt cgcatgatgc gttacgccat tgccgcaatg
    39601 caaaatcacc tggatgccgg atacaaaaca ttgccgatgg tggtgccatt gttgttttac
    39661 cacggtattg aaagccccta tccctattcg ctgtgttggc ttgattgttt cgccgatccc
    39721 aaactggcaa ggcagcttta tgcctccgca tttccgctga ttgatgtcac cgtcatgcct
    39781 gatgatgaaa tcatgcagca ccgacgtatg gcgctgctgg agttaattca aaaacatatt
    39841 cgtcaacgcg atctgatggg gctggtagag caaatggcct gcttattaag tagtggatac
    39901 gctaatgaca gacaaatcaa agggctgttt aattacatct tgcaaaccgg cgatgcggta
    39961 cgttttaacg attttatcga cggcgttgcc gaacattcac cgaaacacaa ggagagctta
    40021 atgactattg cggaaagatt gcggcaagag gggtttcaga aaggtatacg agctatcgcc
    40081 aaaaccatgc tggatgccgg agtccctctt gaggacgttc tgcgatttac cagcctgacg
    40141 gttgaagacc ttgctgaaat aaatcatcaa taacgtaaag gatggtttta atgacagatg
    40201 cagaaagaga atatcttatt agttacctga ttggttattt attgggtcga cgcctggaag
    40261 ccctggatat cgccaaaaga atgctgtaat ccggagtccc tcttgcagac atcatgcgct
    40321 ttaccggggt gtcagaagaa gagttggctg cggcgagcca gtaaagttct gtctcgccat
    40381 ttcaaaagcc acctacaccc tctgcttcaa cgccaccagc atgcgacaaa actcggccgg
    40441 atgcgaaata aacggcgcat gggccgcttt ggcgaagatg tatgattcgc tgtgaggcca
    40501 aagtttatcc agcatcggca ccactttgcg cggcaccaga ccgtcgagat agccatacaa
    40561 tcgcaaaaac ggcatggaca cgttttgcag cggctgacgg agatcgacca ttttcaggat
    40621 ttccagcccg ccattaagca cgtcaacctc cggcatcggt aacgccagaa cggttttctt
    40681 cagcgcccgc gcatcctggc gcgccgtttc agtacccatg gtttgtaacg ccaggaaccg
    40741 ctccaccgta cgctgaaaat catcactgag ttgctgctga aatcccgcca gcacgtccgg
    40801 ttttatcccc ggccactcgt cacgagcact aaaacaaggt gacgacgcca cggtgaccag
    40861 cgcctgaaca cgctcgggat gggttaacgc aatctggctt gccaccagcc cgcccagact
    40921 ccagcctaac caaatggctt tatcaggtgc ctgtcgcagc acggcttcgg ccatatcagc
    40981 aagtgacatc gcaccaaatc cccggctacg cccgaagccg ggcagatcaa caaggtgcag
    41041 cgtaaaatgc gagctcagtt cctcgtcaat gcaacgccac acttcggcat tcagtcccca
    41101 tccgtgcagc agcacaagat gaacattccc ctgacctttg gtctgccacc agatgttatt
    41161 catccgctat tgttctcttt tgacttacaa ggatgaacat atgctaacag taccgggatt
    41221 atgctggcta tgccgaatgc cgctggcgtt aggtcattgg gggatttgtt cggtatgctc
    41281 acgcgccgcc cgtacagata aaacgttatg cccccaatgt ggattacccg ccacacactc
    41341 acatcttccc tgcggtcgct gcctgcaaaa accgccgccc tggcaaagac tggtcacggt
    41401 tgccgactat gcgccgccgt taagtccgct tatccaccag cttaagtttt cccggcgcag
    41461 cgaaatcgcc agcgccctgg cacgtctgtt actattggaa gtcttacacg ctcgtcgcac
    41521 caccgggttg caattgccgg atcgcattat cagcgttccg ttatggcagc ggcgtcactg
    41581 gcgtcgggga tttaatcaga gcgatttgct gtgtcagccg ttatcacgct ggttgcactg
    41641 ccgatgggat agcgaagccg tcacacgaac acgggccact gcgacccagc attttctcag
    41701 tgcccggctg cgcaagcgca acctgaaaaa tgcctttcgc cttgaattgc ctgtgcaagg
    41761 tcgccatatg gtgattgtgg atgatgtcgt caccaccgga agtaccgtcg cagagattgc
    41821 gcagttgctt ttacgcaatg gtgcggcgac tgtccaggtc tggtgccttt gtcgaacctt
    41881 gtagagcctc gatgatgggc gtattataac caactaaaat agtcaactat taggccatta
    41941 ctatgatccg tatttccgat gctgcacaag cgcactttgc caaactgctg gcaaatcagg
    42001 aagaagggac acaaatccgc gtatttgtga ttaaccctgg cacgcctaac gctgaatgtg
    42061 gcgtttctta ttgtccgccg gacgctgtag aagccaccga cactgccctg aaatttgacc
    42121 tgctgaccgc gtatgttgat gagttaagcg ctccttacct ggaagatgca gagatcgact
    42181 ttgttacaga ccagttgggt tcccagttaa cgctgaaagc gcctaacgcc aaaatgcgta
    42241 aagtcgcaga cgatgcaccg ctgatggagc gcgtggagta tatgctgcag tcgcagatca
    42301 acccacagct tgctggtcac ggtggtcgcg tttcgctgat ggaaatcacc gaagacggtt
    42361 acgccattct gcaatttggc ggcggctgta acggttgttc tatggtagat gtgacgctga
    42421 aagaagggat cgagaagcag ctgctgaacg aattcccgga gctgaaaggt gtgcgcgatc
    42481 tcaccgaaca ccagcgcggc gagcactcct actactaagt tatcccctct tttgtagatt
    42541 gcccgatgcg acgctaaagc gtcatttcgg gcctacgttg aacatgtgcc agacgttggc
    42601 gcagcattgc tttggcaaac ccctcccttc gtttccccgc gataatatga ccaacctctc
    42661 ataatttaaa tttaccccgc tctggtgatt ctcaaacgcc ggatgttacc cgtatcattc
    42721 acgtgggtac caaacatact cctgacatct gactacaata attagtttta gtgggtatca
    42781 gtcgtggtgc cgcaatatct ctgttcccga ttgggataat tagagtttgt cgtcagaaaa
    42841 ttgacgttac ccataacaaa tgaaaggcca ggtaaatcat gccattagtc attgttgcta
    42901 tcggtgtaat cttgttgttg ctcctgatga tccgtttcaa aatgaacggc ttcatcgctc
    42961 tcgtcctcgt ggcgcttgct gttggattaa tgcaaggaat gccgctggat aaagttattg
    43021 gctccatcaa agcaggtgtc ggcggaacgc tcggtagcct tgccctgatc atgggttttg
    43081 gcgcaatgct gggcaaaatg ctggcagact gcggtggcgc acaacgtatc gccaccacgc
    43141 tgattgctaa atttggtaaa aaacacatcc agtgggcggt ggtactgacc ggttttaccg
    43201 ttggttttgc cctgttctat gaagtgggct ttgtgctgat gctgccgctg gtgtttacca
    43261 tcgcggcttc cgcgaatatt ccactgctgt atgttggtgt accaatggcg gctgcactgt
    43321 ctgtgaccca cggcttcctg ccaccgcatc cgggcccgac tgcgattgcc accattttca
    43381 atgccgatat gggtaaaacc ctgctgtacg gtactattct ggcaatcccg accgtgattc
    43441 tcgccggtcc ggtttacgct cgcgtgctga aaggtataga taagccgatc ccggaaggtc
    43501 tgtacagcgc gaaaaccttc agcgaagaag agatgccaag ttttggcgtc agcgtctgga
    43561 cctctctggt cccggttgtg ctgatggcga tgcgtgcgat tgccgaaatg atcctgccga
    43621 aaggtcacgc tttcctgccg gtagcggagt tcctcggtga cccggtaatg gctacgctga
    43681 ttgccgtact gattgcgatg ttcacctttg gtctgaaccg tggtcgttca atggatcaga
    43741 ttaacgacac gctggtttct tccatcaaaa tcattgcgat gatgctgttg attatcggtg
    43801 gtggcggtgc gttcaagcag gtgctggtag acagcggcgt ggacaaatac attgcttcca
    43861 tgatgcacga aaccaacatt tctccgctgc tgatggcctg gtcgattgct gccgtactgc
    43921 gtatcgcgct gggttccgca accgttgcgg caatcactgc gggtggtatc gcggcaccgc
    43981 tgattgcaac gacgggtgtt agcccggagc tgatggttat tgcggttggt tccggtagtg
    44041 tgattttctc tcacgtgaac gatccgggct tctggctgtt caaagagtac tttaacctga
    44101 ctatcggcga gaccatcaaa tcctggtcga tgctggaaac cattatctcg gtgtgcggtc
    44161 tggtaggctg tctgctgctg aatatggtga tttgataaca ctgactgccg gatgcatcgc
    44221 atccggcatc aaccgcactc tacttcttct tcgctgccgc tctgcgccgt ctgtccaaat
    44281 ccttcagcaa cttgttcaca ccatcatcgg caaacatcgc ctcaagcgtt gcggagagct
    44341 tgcgccgcca gtttttatac tggtaactgg tgccgggaat atttaccggg tccgccatat
    44401 ccagccagtc ttctggctgt agtcctaaca gggcgctgtt gctatcggcg atgtagcgct
    44461 gtaagccacg attcagcgtc ggtgtcatcg acatcaacga tgccttatgc ccggcacgtt
    44521 ttggcaggca accatattta tgcagagcat ccagtagtcc ttgcttcgcc agttcgcgat
    44581 cctgatacag accgcgcagt accacttcat ccggatacag ccccagcgtt ttgcccagcg
    44641 ttaaatcccc gctctcccag tagccgcgta gcgtaggcag gtcgtgtgtc gccgcaaccg
    44701 ccatcgactg ctctgggtac gctttcggtg cgcggaatgt cttctcgtgg tcgttttcga
    44761 aatagagcac tttgtaggag tacacaccgc tgctgcgcag cttaccgaca atctctaccg
    44821 gtacggtacc gagatcttca ccaatcacca tacagcgatg acgtttactt tcgagtgcca
    44881 gaatcgagag cagatcatcc accggatagt gaacatacgc gccctgatct gccgtctcgc
    44941 catacggtat ccaccacaaa cgcagcatcg acatcacgtg atcaattcgt aatgcgccgc
    45001 agttttgcat attggcacgc aacagctcga taaacggttc ataggcacgc gcggtgataa
    45061 tatgcgggtc cattggcggc aatccccagt tctgccccaa cgggccgagg atatccggcg
    45121 gtgctccaac cgacgctttc aggcaataca actcccggtc gcaccaggtt tctgatccac
    45181 cctctgctac accaaccgcc agatcacgat acaagccaat cggcatttca tagccctggc
    45241 ttatctccca gcaggcggca aactggctgt aagccagcca ctgcaaccag agataaaaat
    45301 cgacgtcatc acgatgttct tcgcagaact ggcgcacttc tggtgaatcc acgttctgat
    45361 acatctctgg ccatgcaggc cagccccagc gcatttcgtc ctctttcact tgctgggcat
    45421 gtagcgcgtc aaacgccgcc tgccagaaca ggctatcgcc ttgctcggca acaaactgac
    45481 gaaacgcggc cacctgttca tcatcacgtt gcgcgaaacc tttccacgcc atgcgcaatg
    45541 ctgtcatttt tagggcggta accgtggagt aatcgaccca gtcggcatcc cgcgtctgtt
    45601 gcagcgtctg ttgcgtggtc ggcaacttcc accaggcctg agcctcttcg ctaagatgga
    45661 aatcttcgac ggcgttaacg tcgatataaa tcacattcag ccaacggcga gaagacgggc
    45721 tgtatgggct ggcactttcc ggatttgccg gatagagcgc atgaatcggg ttcagaccga
    45781 tgaacgaccc gccacgtttt gccacatcca ccagcatcgc tttgagatcg ccaaaatccc
    45841 caatacccca gtttttttcc gatcgcagcg tataaagctg aacgcaggca ccccacagct
    45901 tttgtttatt cagcaacgcc tgcggttcgt aacagcgttt cggggcgaca atcaccctgc
    45961 aatgcacgcg ctggtcttcc tgggtaaacg tcagcgtgtg ataaccttcc ggcagcttcg
    46021 tcggtagatt gaacgctttg ccccccgtta catggccttt gtactgcgtt ccttcttcgg
    46081 tggtcagcag ccagctatat tcgccgctgc cctccaccac catcggcatt tttttgccgc
    46141 tggtataaac catgacattc gggactggcg ttaccgccac tttcgtggcg gtacgttgat
    46201 gcatcgcgtc aagcaaacgc cgtttggttt cggcgctaat cgactgcggt ttaccgtggg
    46261 cattgatgta attggggcta atccccgccg ccagcgcggc attatccaga cgtttgcttt
    46321 ccatcgagct tccttagcgt tttgcctgcc agatacgagc ctgataatcg cgaatagagc
    46381 gatccgagct aaacataccg cagcgggcgg tattgaggat cgccgcgcga gtccaggcct
    46441 cctggtcgcg gtacagcaca tccacctgct tttgtgcctc tacataggct gcgaaatccg
    46501 ccatcaccag atacggatcg ccgccctgtt tgccgatact gtgcagcatc tgatcgaagg
    46561 catgcttatc gccgtcgctg tatttgccgc tttccagctc tttcaatact gcgtccagca
    46621 ccttatcttt cttccgccat ttcaccgggt cgtagccttt ggccagaatt gccttcactt
    46681 gttccacggt atgaccaaaa ataaagatat tttcttcacc aactttctcg gcgatttcaa
    46741 cgttcgcacc atccagcgta ccgacagtaa gcgcaccatt gagcgccagt ttcatattgc
    46801 cggtaccgga agcttcttta cctgccgtcg aaatttgttc ggagatatcc gccgccggga
    46861 tcagtttttc cgccgccgaa acgcaataat ccggcaggaa caccaccttc aatttgtcgc
    46921 caacctgcgg atcgttgttg atcacgtcag ccactttgtt gatcgcaaag ataatgtttt
    46981 tcgccaggta gtagcccggt gccgctttcg cgccaaagag gaagacgcgc ggtacgcgat
    47041 cagcctgcgg gttttcacga atttctttgt acaacgccag aatatgcagc agattcaggt
    47101 gctggcgttt gtactcatgc agacgtttga tctgaatatc gaaaatcgcc tgtggattga
    47161 tctcaatacc ggtacgaact ttcacaaact ctgccagacg gactttattc gcctgcttga
    47221 tctcgcgata ttgctgacgg aatttcgcat catcagcgaa tttttccaga ttgatcagtt
    47281 gatcgagatc gttagtccac tcttttttca gtgatttatc caacagagcc gccagtgccg
    47341 ggttgcactg tttgatccag cgacgtgggg taataccgtt ggtgacgtta tggaatttgt
    47401 tcggccatag ctggtgatat tccgggaaca gatctttcac caccagatcc gagtgcaggg
    47461 ccgcaacacc gtttaccgcg aaaccgccaa ccacacacag gttcgccata tgcacttgtt
    47521 tgtcgtgtac caccgccagt ttggcccata ctttttcatc gcccggccag gttttttcca
    47581 ccagcgtttt aaagcgtgta ttaatttcgt taataatctg catgtggcgc ggcagtaagc
    47641 ctttcaccag tttcacatcc cagcgttcca gcgcttctgg catcaaggta tggttggtgt
    47701 aagcgaaagt tttactggtg atggcccagg catcatccca gctcatctgg tgctcatcga
    47761 tcagcacgcg cagcagttct ggaatcgcga tagttgggtg ggtatcgttc agctgaataa
    47821 cttcgtactc cgccagctcg tgcagtttac gccccgccag atgatggcga cgcaaaatat
    47881 ccgctaccga gcaggcacac tggaagtatt gctgcatcag gcgcagcttt ttaccggcag
    47941 tatggttgtc gtttggatag agaactttgg tcagtttttc cgcgttgatg ccctgctgtt
    48001 cggcacgcag gaaatcacca tcgttaaatt tagtcagatc aaacggatgc gcatgcgtcg
    48061 cctgccacag acgcagcggc tgcgccacgc cattacgata gccgacaacg gggagatccc
    48121 acgcttgacc ggtaatggta aactccggct cccagcgtcc gtctttcgtc actttaccgc
    48181 caatccctac ctgcacatcc agtgcttcgt tgtggcggaa ccacgggtag ttactgcgat
    48241 gccagtcatc cggcgcttca acctgtttgc catcgacaaa tgactggcgg aacaagccat
    48301 attgataatt aaggccgtaa ccagtagctg actgcccgac agttgccatt gagtcgagga
    48361 agcacgccgc cagacgtccc agaccaccgt tacccagcgc cgggtcgatc tcttcttcca
    48421 gcaagtcagt caggttgatg tcataagcct tcaacgaatc ctgtacatcc tgataccagc
    48481 cgagattcaa cagattgttg cccgtcaggc gaccaatcaa aaactccatt gagatgtagt
    48541 taacatgccg ctgattcgcc accggtttgg caaatggctg agcacgcagc atttcggcca
    48601 gtgcttcgct cactgccaac caccactgac gaggggtcat ttcagccgca gaatttaagc
    48661 cataacgctg ccactgacgt gaaagcgctt cctgaaattg cttatcgtta aaaataggtt
    48721 gtgacatagg agttccactt ttcttagatt ttcaacacaa cgttatcgct agtttgccag
    48781 gctcgatgtt gaccttcctc atcctgcggg ggattaggca gggaggagtt gcggggatga
    48841 gcaaggaaat gtgatctcga ccacttaaag ctagtgcaaa ccacaggatt agcagcaaat
    48901 caatgccata cagcgcagaa aatctgtatc aaagtgcaaa aaatggccgt tgcgtatttt
    48961 caaaaagcgg aaggtaactc tataattaag taaaggagtg aaacagtttc acaagtaaaa
    49021 tatccagtgt gctccatctc attcttaata gatttattaa gatcatcttt ttagatggca
    49081 ctttcatcag aaatgaagaa gaaacccttg cttaaatgaa actgatgaac ataaggaaaa
    49141 taccgtacaa cccagtattc acgctggatc agcgtcgttt taggtgagtt gttaataaac
    49201 atttggaatt gtgacacagt gcaaattcag acacataaaa aaacgtcatc gcttgcatta
    49261 gaaaggtttc tggccgacct tataaccatt aattacgaag cgcaaaaaaa ataatatttc
    49321 ctcattttcc acagtgaagt gattaactat gctgattccg tcaaaattaa gtcgcccggt
    49381 tcgactcgac cataccgtgg ttcgtgagcg cctgctggct aaactttccg gcgcgaacaa
    49441 cttccggctg gcgctgatca caagtcctgc gggctacgga aagaccacgc tcatttccca
    49501 gtgggcggca ggcaaaaacg atatcggctg gtactcgctg gatgaaggtg ataaccagca
    49561 agagcgtttc gccagctatc tcattgccgc cgtgcaacag gcaaccaatg gtcactgcgc
    49621 gatatgtgag acgatggcgc aaaaacggca atatgccagc ctgacgtcac tcttcgccca
    49681 gcttttcatt gagctggcgg aatggcatag cccactttat ctggtcatcg atgactatca
    49741 tctgatcact aatcctgtga tccacgagtc aatgcgcttc tttattcgcc atcaaccaga
    49801 aaatctcacc cttgtggtgt tgtcacgcaa ccttccgcaa ctgggcattg ccaatctgcg
    49861 tgttcgtgac cagctgctgg aaattggcag tcagcaactg gcatttaccc atcaggaagc
    49921 gaagcagttt tttgattgcc gtctgtcatc gccaattgaa gccgcagaaa gcagtcggat
    49981 ttgcgatgac gtttccggtt gggcgacggc actgcagcta atcgccctct ccgcccggca
    50041 gaatactcac tcagcccata agtcggcacg ccgcctggcg ggaatcaatg ccagccatct
    50101 ttcggattat ctggtcgatg aggttttgga taacgtcgat ctcgcaacgc gccattttct
    50161 gttgaaaagc gccattttgc gctcaatgaa cgatgcactc atcacccgtg tgaccggcga
    50221 agaaaacggg caaatgcgcc tcgaagagat tgagcgtcag gggctgtttt tacagcggat
    50281 ggatgatacc gacgattggt tttgctatca cccactgttt ggtaacttcc tgcgccagcg
    50341 ctgccagtgg gaactggcgg cggagctgcc ggaaatccac cgtgccgccg cagaaagctg
    50401 gatggcccag ggatttccca gcgaagcgat tcatcatgcg ctggcggcag gcgatgcgct
    50461 gatgctgcgc gatattctgc ttaatcacgc ctggagtttg ttcaaccata gcgaactgtc
    50521 gctgctggaa gagtcgctta aggccctgcc gtgggacagt ttgctggaaa atccgcagtt
    50581 ggtgttattg caggcgtggc tgatgcaaag ccagcatcgc tacggcgaag ttaacaccct
    50641 actggcccgc gctgaacatg aaatcaagga cattagagaa ggcaccatgc acgcagaatt
    50701 taacgctctg cgcgcccagg tagcgattaa cgatggtaat ccggatgaag cggaacggct
    50761 ggcaaaactg gcactggaag agctgccgcc gggctggttc tatagccgca ttgtggcaac
    50821 ctcggtgctg ggtgaagtgc tgcactgcaa aggcgaattg acccgctcac tggcgctaat
    50881 gcagcaaacc gaacagatgg cacgccagca cgacgtctgg cactacgcct tatggagttt
    50941 aatccagcaa agtgaaattc tgtttgctca gggattcctg caaaccgcgt gggaaacgca
    51001 ggaaaaagcg ttccagctga tcaacgagca gcatctggaa cagctgccaa tgcatgagtt
    51061 tctggtgcgc atccgagcac aactgttatg ggcctgggca cggctggatg aagccgaagc
    51121 gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag ccacagcaac agcttcagtg
    51181 cctggcaatg ttgattcaat gctcgctggc ccgtggtgat ttagataacg ctcgtagcca
    51241 gcttaaccgt ctggaaaacc tgctggggaa tggcaaatat cacagcgact ggatctctaa
    51301 cgccaacaaa gtccgggtga tttactggca aatgaccagc gataaagccg ccgctgccaa
    51361 ctggttgcgt catacggcta aaccggagtt tgcgaacaac cacttcctgc aaggtcaatg
    51421 gcacaacatt gcccgtgcac aaatcttgct gggcgagttt gaaccggcag aaattgttct
    51481 cgaagaactc aatgaaaacg cccgcagtct gcggttgatg agcgatctca accgtaacct
    51541 gttgctgctt aatcaactgt actggcaggc cggacgtaaa agtgacgccc agcgtgtgtt
    51601 gctggacgca ttaaaactgg cgaatcgcac cggatttatc agtcattttg tcatcgaagg
    51661 cgaagcgatg gcgcaacaac tgcgccagct tattcaactc aatacgctgc cggagctgga
    51721 acagcatcgc gcacagcgta ttctgcgaga aatcaatcaa catcatcggc ataaattcgc
    51781 ccatttcgat gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc cagaactgat
    51841 ccgcaccagc ccgttgaccc aacgtgaatg gcaggtcctg gggctgattt actccggtta
    51901 cagcaacgag caaattgcag gtgagctgga agtggcggca accaccatca agacgcatat
    51961 ccgcaatctg taccaaaaac tcggtgtcgc ccaccgtcag gccgcggtac aacacgcgca
    52021 gaaactgctg aagatgatgg gatatggggt gtgagtttag ccggataata cgtcggatcc
    52081 ggcttacatc tttatatcat tcaatgctca cccgcgttac gccatccgcc tccaccagac
    52141 taaaccgcac tggcaagaaa cgctccacca ccgcaatatt ggtcagcaga tggcatgagg
    52201 gatgggcgac cgtaaattct cccgcgcccg ccagcgccat cggcagtacc agctggtcgg
    52261 cgagatattc ccccaccgcc gccggacttg ccaggtagcg tttcacctct ttcaccaact
    52321 gtgccgcgac cacttcggcg ctgacgcgct tttcaccgac gacaaaaaag cgttcggtga
    52381 tattttcact ttcgacttca agcgaaacgg tattacccgg tccctggtca cgcggcaggt
    52441 tatgaatatt ctgctcatgc agggaaaaac tccccgccag tgtagcgatt tcacgctcag
    52501 cgacatggcg cggcacgcca gctaatagca cctctccacg catctgcaca atgttcccgc
    52561 gctcgccaag ttgcaaggtg ttaaacaatg ccaccggcga gacttccgtt gccaccaccc
    52621 cgcctccagc aggataaaaa ccgtggcgta ataatgtggt ttgctgatga atgcccattt
    52681 tcgccagcag cggctccagc acccggcgga taaaatccgc aggcggtgcc gacgggttat
    52741 cggtgccgcc gctcacttca acacgcgaag gcccatcagc aaaccacagc gcgggtagca
    52801 ccgtttgcag caccagcgtg ctgcttccgg cactaccgat agcaaagcgg taatcgccgc
    52861 cacgcacggt accgggccgg aagaccagac gctgtgaccc cagctccgcg ccttccaccg
    52921 ttgccctaca aatttccgca gccgctttta ccgcggtcag atgttggcgc aacagccccg
    52981 gtttcgcccg cccggcacga atgccggtga tggtaaatgg ctggccggtt atcatcgaca
    53041 ggctcagcgc cgagcgcagg atctgcccgc cgccttcgcc ctgtgcgcca tccagcgcaa
    53101 tcatcctttt catcatttat ccttttacgc acaccacctg acgcagggta tagataactt
    53161 ccaccagatc gctttgtgcc gccatcaccg catcaatatc tttatacgcc atcgggattt
    53221 cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc ggtggcacga atttgatctt
    53281 ccacgctgaa catttttttc gctttagtac ggctcataac ccgcccggca ccgtggctgc
    53341 acgaacagaa cgactcttca tttcccagcc cacggacgat aaagcttttc gctcccatcg
    53401 accctggaat aattccatat tgaccagcac gcgcagacac cgcgccttta cgcgtcacgt
    53461 agatctcttc accaaagtgc tgttcttttt gcacatagtt gtggtgacag ttgatctctt
    53521 ccatcgccag cgtttgtggc tgtctgaccg ttttctgcgt aatgctctgc aacgccgtta
    53581 ccacgttttc catcatcgca tcgcggttaa ggctggcaaa aagctgcgcc caggccacgg
    53641 ctttcaggta atcatcaaag tattccgtac cttccataaa gtacgccaga tcacgcgacg
    53701 gcaatgtttc aagcgtttcc tgcatctctt tttgcgccag atcgataaag taagtcccga
    53761 tggcgttacc aattccgcgt gaaccggagt gcagcataat ccacacctgg tccgactcat
    53821 caaggcagat ttcaataaag tggttacccg ttcccagcgt tcccaggtgt ttatagttgt
    53881 tagtgttaag gaaacgggga tatttttgcg ttaaccactg ataaccggct tccagctcag
    53941 cccatttcgc atcgacgtta acaggcggat tttcccatgc gcctttatca cgtttacaac
    54001 gtccggtagt acgtccgtgc ggcacggctg tttcaatcgc ctgacgcagc tccgccaggt
    54061 tttccggcag gtctgccgcc gttaacgcgg tacgcagcgc gttcattcca caaccgatat
    54121 ccacgcccac cgccgccgga ataatcgccc ctttggtcgg gatcacgcta ccaatggtgg
    54181 aacctttacc caggtgtaca tccggcatta ccgcaatatg tttgaaaata aacggcatct
    54241 tcgccgtatt aataagttgc tgacgcgcat cggcctctac cggcacgcct ttggtccaca
    54301 tttttaccgg ggcattttca gtggtcagta attcgtaatt cattttgttt ttctcttt
//
LOCUS       DAZUCC010000006        53079 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZUCC010000006 DAZUCC010000000
VERSION     DAZUCC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.006"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..422
                     /gene="cmtB"
                     /locus_tag="ACHY09_000270"
     CDS             <1..422
                     /gene="cmtB"
                     /locus_tag="ACHY09_000270"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFS3883290.1"
                     /translation="ESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQAIKDSTIN
                     NGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIGLSAADADS
                     HIGAIQALSELLCEEEILNQLLTAASEKQLADIISRG"
     gene            450..1838
                     /gene="cmtA"
                     /locus_tag="ACHY09_000271"
     CDS             450..1838
                     /gene="cmtA"
                     /locus_tag="ACHY09_000271"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFS3883291.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSVI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKCVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKN"
     gene            1853..3130
                     /gene="yggP"
                     /locus_tag="ACHY09_000272"
     CDS             1853..3130
                     /gene="yggP"
                     /locus_tag="ACHY09_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFS3883292.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTDKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3127..3442
                     /locus_tag="ACHY09_000273"
                     /pseudo
     CDS             3127..3442
                     /locus_tag="ACHY09_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            3464..3973
                     /gene="fumE"
                     /locus_tag="ACHY09_000274"
     CDS             3464..3973
                     /gene="fumE"
                     /locus_tag="ACHY09_000274"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFS3883293.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSRMVKTALTLAV
                     TTTLKELTP"
     gene            3970..4683
                     /gene="yggC"
                     /locus_tag="ACHY09_000275"
     CDS             3970..4683
                     /gene="yggC"
                     /locus_tag="ACHY09_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFS3883294.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WQELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQEAEAFYARTDGPNVERVLVNS
                     RQANLILEMTEEGRYHFKS"
     gene            4968..5987
                     /gene="epd"
                     /locus_tag="ACHY09_000276"
     CDS             4968..5987
                     /gene="epd"
                     /locus_tag="ACHY09_000276"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFS3883295.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6037..7200
                     /gene="pgk"
                     /locus_tag="ACHY09_000277"
     CDS             6037..7200
                     /gene="pgk"
                     /locus_tag="ACHY09_000277"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS3883296.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            7414..8493
                     /gene="fbaA"
                     /locus_tag="ACHY09_000278"
     CDS             7414..8493
                     /gene="fbaA"
                     /locus_tag="ACHY09_000278"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS3883297.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            8684..9544
                     /gene="mscS"
                     /locus_tag="ACHY09_000279"
     CDS             8684..9544
                     /gene="mscS"
                     /locus_tag="ACHY09_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFS3883298.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            9683..10318
                     /gene="argO"
                     /locus_tag="ACHY09_000280"
     CDS             9683..10318
                     /gene="argO"
                     /locus_tag="ACHY09_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFS3883299.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIVHAQALLS"
     gene            10411..11151
                     /gene="yggE"
                     /locus_tag="ACHY09_000281"
     CDS             10411..11151
                     /gene="yggE"
                     /locus_tag="ACHY09_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFS3883300.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            11319..12215
                     /gene="ygfI"
                     /locus_tag="ACHY09_000282"
     CDS             11319..12215
                     /gene="ygfI"
                     /locus_tag="ACHY09_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3883301.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(12212..13690)
                     /gene="scpC"
                     /locus_tag="ACHY09_000283"
     CDS             complement(12212..13690)
                     /gene="scpC"
                     /locus_tag="ACHY09_000283"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFS3883302.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPNAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPSAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(13714..14499)
                     /gene="scpB"
                     /locus_tag="ACHY09_000284"
     CDS             complement(13714..14499)
                     /gene="scpB"
                     /locus_tag="ACHY09_000284"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFS3883303.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(14510..15505)
                     /gene="meaB"
                     /locus_tag="ACHY09_000285"
     CDS             complement(14510..15505)
                     /gene="meaB"
                     /locus_tag="ACHY09_000285"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFS3883304.1"
                     /translation="MINEAKLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNRLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(15498..17642)
                     /gene="scpA"
                     /locus_tag="ACHY09_000286"
     CDS             complement(15498..17642)
                     /gene="scpA"
                     /locus_tag="ACHY09_000286"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFS3883305.1"
                     /translation="MSKVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSAAE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGRKDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(17846..18739)
                     /gene="argP"
                     /locus_tag="ACHY09_000287"
     CDS             complement(17846..18739)
                     /gene="argP"
                     /locus_tag="ACHY09_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFS3883306.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18880..19065
                     /locus_tag="ACHY09_000288"
     CDS             18880..19065
                     /locus_tag="ACHY09_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883307.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFASWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            19121..19780
                     /gene="rpiA"
                     /locus_tag="ACHY09_000289"
     CDS             19121..19780
                     /gene="rpiA"
                     /locus_tag="ACHY09_000289"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS3883308.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20036..21268
                     /gene="serA"
                     /locus_tag="ACHY09_000290"
     CDS             20036..21268
                     /gene="serA"
                     /locus_tag="ACHY09_000290"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS3883309.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLAEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            21497..21556
                     /gene="ibsC"
                     /locus_tag="ACHY09_000291"
     CDS             21497..21556
                     /gene="ibsC"
                     /locus_tag="ACHY09_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFS3883310.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(21657..22259)
                     /gene="fau"
                     /locus_tag="ACHY09_000292"
     CDS             complement(21657..22259)
                     /gene="fau"
                     /locus_tag="ACHY09_000292"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS3883311.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTLPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(22280..22463)
                     /gene="ssrS"
                     /locus_tag="ACHY09_000293"
     ncRNA           complement(22280..22463)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHY09_000293"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22505..22834)
                     /gene="zapA"
                     /locus_tag="ACHY09_000294"
     CDS             complement(22505..22834)
                     /gene="zapA"
                     /locus_tag="ACHY09_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS3883312.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALFEQGRITE
                     KTNQNFE"
     gene            23002..23580
                     /gene="ygfB"
                     /locus_tag="ACHY09_000295"
     CDS             23002..23580
                     /gene="ygfB"
                     /locus_tag="ACHY09_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFS3883313.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23606..24931
                     /gene="pepP"
                     /locus_tag="ACHY09_000296"
     CDS             23606..24931
                     /gene="pepP"
                     /locus_tag="ACHY09_000296"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFS3883314.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            24928..26106
                     /gene="ubiH"
                     /locus_tag="ACHY09_000297"
                     /gene_synonym="visB"
     CDS             24928..26106
                     /gene="ubiH"
                     /locus_tag="ACHY09_000297"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFS3883315.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGCVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWGPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
                     "
     gene            26129..27331
                     /gene="ubiI"
                     /locus_tag="ACHY09_000298"
                     /gene_synonym="visC"
     CDS             26129..27331
                     /gene="ubiI"
                     /locus_tag="ACHY09_000298"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFS3883316.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(27405..27488)
                     /locus_tag="ACHY09_000299"
     CDS             complement(27405..27488)
                     /locus_tag="ACHY09_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883317.1"
                     /translation="MSKNTKSKIHNIRKYNAKTEVKLVYFK"
     gene            27779..28873
                     /gene="gcvT"
                     /locus_tag="ACHY09_000300"
     CDS             27779..28873
                     /gene="gcvT"
                     /locus_tag="ACHY09_000300"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFS3883318.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            28897..29286
                     /gene="gcvH"
                     /locus_tag="ACHY09_000301"
     CDS             28897..29286
                     /gene="gcvH"
                     /locus_tag="ACHY09_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFS3883319.1"
                     /translation="MSNVPAELKYSKEHEWLRKEAGGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            29405..32278
                     /gene="gcvP"
                     /locus_tag="ACHY09_000302"
     CDS             29405..32278
                     /gene="gcvP"
                     /locus_tag="ACHY09_000302"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFS3883320.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(32442..33875)
                     /gene="bglA"
                     /locus_tag="ACHY09_000303"
     CDS             complement(32442..33875)
                     /gene="bglA"
                     /locus_tag="ACHY09_000303"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFS3883321.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLHEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            33920..34231
                     /gene="yqfB"
                     /locus_tag="ACHY09_000304"
     CDS             33920..34231
                     /gene="yqfB"
                     /locus_tag="ACHY09_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFS3883322.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTDLKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            34395..35054
                     /gene="yqfA"
                     /locus_tag="ACHY09_000305"
     CDS             34395..35054
                     /gene="yqfA"
                     /locus_tag="ACHY09_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS3883323.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     IKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(35131..36111)
                     /gene="ygfZ"
                     /locus_tag="ACHY09_000306"
     CDS             complement(35131..36111)
                     /gene="ygfZ"
                     /locus_tag="ACHY09_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFS3883324.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            36364..36630
                     /gene="sdhE"
                     /locus_tag="ACHY09_000307"
     CDS             36364..36630
                     /gene="sdhE"
                     /locus_tag="ACHY09_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFS3883325.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            36611..37018
                     /gene="ygfX"
                     /locus_tag="ACHY09_000308"
     CDS             36611..37018
                     /gene="ygfX"
                     /locus_tag="ACHY09_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFS3883326.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(37058..37579)
                     /gene="fldB"
                     /locus_tag="ACHY09_000309"
     CDS             complement(37058..37579)
                     /gene="fldB"
                     /locus_tag="ACHY09_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFS3883327.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            37691..38587
                     /gene="xerD"
                     /locus_tag="ACHY09_000310"
     CDS             37691..38587
                     /gene="xerD"
                     /locus_tag="ACHY09_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS3883328.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            38612..39322
                     /gene="dsbC"
                     /locus_tag="ACHY09_000311"
     CDS             38612..39322
                     /gene="dsbC"
                     /locus_tag="ACHY09_000311"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFS3883329.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            39328..41061
                     /gene="recJ"
                     /locus_tag="ACHY09_000312"
     CDS             39328..41061
                     /gene="recJ"
                     /locus_tag="ACHY09_000312"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS3883330.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHSGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGVA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAL
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLTYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            41152..42250
                     /gene="prfB"
                     /locus_tag="ACHY09_000313"
     CDS             join(41152..41226,41228..42250)
                     /gene="prfB"
                     /locus_tag="ACHY09_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS3883331.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            42260..43777
                     /gene="lysS"
                     /locus_tag="ACHY09_000314"
     CDS             42260..43777
                     /gene="lysS"
                     /locus_tag="ACHY09_000314"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS3883332.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(43820..44368)
                     /gene="idi"
                     /locus_tag="ACHY09_000315"
     CDS             complement(43820..44368)
                     /gene="idi"
                     /locus_tag="ACHY09_000315"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS3883333.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     YYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            44423..44494
                     /locus_tag="ACHY09_000316"
     CDS             44423..44494
                     /locus_tag="ACHY09_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883334.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(44491..44616)
                     /gene="yqfG"
                     /locus_tag="ACHY09_000317"
     CDS             complement(44491..44616)
                     /gene="yqfG"
                     /locus_tag="ACHY09_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFS3883335.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(44618..46066)
                     /gene="uacT"
                     /locus_tag="ACHY09_000318"
     CDS             complement(44618..46066)
                     /gene="uacT"
                     /locus_tag="ACHY09_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFS3883336.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            46501..48420
                     /gene="ygfT"
                     /locus_tag="ACHY09_000319"
     CDS             46501..48420
                     /gene="ygfT"
                     /locus_tag="ACHY09_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFS3883337.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            48420..48908
                     /gene="ygfS"
                     /locus_tag="ACHY09_000320"
     CDS             48420..48908
                     /gene="ygfS"
                     /locus_tag="ACHY09_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFS3883338.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVSACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(48944..50311)
                     /gene="ghxQ"
                     /locus_tag="ACHY09_000321"
                     /gene_synonym="ygfQ"
     CDS             complement(48944..50311)
                     /gene="ghxQ"
                     /locus_tag="ACHY09_000321"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFS3883339.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(50347..51663)
                     /gene="guaD"
                     /locus_tag="ACHY09_000322"
     CDS             complement(50347..51663)
                     /gene="guaD"
                     /locus_tag="ACHY09_000322"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFS3883340.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(51681..>53079)
                     /gene="xanQ"
                     /locus_tag="ACHY09_000323"
     CDS             complement(51681..>53079)
                     /gene="xanQ"
                     /locus_tag="ACHY09_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFS3883341.1"
                     /translation="SDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPALI
                     VGATLQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIAL
                     GSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGLS
                     LIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGLC
                     VGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGDI
                     TATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGVA
                     SRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGLK
                     RRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGYR
                     QENVLPGITSAEEMD"
BASE COUNT        12927 a        13208 c        14126 g        12818 t
ORIGIN      
        1 cagaatcatc aatctcagtt atacattcag caaaagattg gcaggaagct atcgatttct
       61 cgatggcatc attgctggat aaaaactata tcagcgagaa ttacattcag gcaattaaag
      121 attccaccat taacaatggc ccttattata ttctcgcacc aggcgtggca atgcctcatg
      181 ctcgaccgga atgtggggcg cttaaaaccg ggatgtcttt aacattactt aaacaaggcg
      241 tttattttcc ggggaatgac gaaccgataa aattactcat cgggctttct gctgccgatg
      301 ccgattcgca cattggcgct attcaggcgt taagtgaatt attgtgtgaa gaagaaatac
      361 tcaaccaatt attaacagca gcatcagaaa aacaattagc ggacataatc agccgcggat
      421 aattactctc tcctctacag gataatatta tggaaaacaa gtctgctcgt gcaaaggtcc
      481 aggcttttgg gggctttttg actgcaatgg tcatccccaa tatcggcgct tttatagcct
      541 ggggttttat aacggcgtta tttattccca ccggctggct gcctaacgaa catttcgcca
      601 aaattgtcgg cccgatgatt acctatttat tgcccgtgat gattggttct accggtggtc
      661 atctggtcgg cggtaaacgc ggcgcggtca tgggcggaat aggtactatt ggtgtgatcg
      721 tcggcgcaga gatcccgatg ttccttggct caatgattat ggggccgctc ggtggtctgg
      781 tgattaaata tatcgacaag gcgctggaaa agcggatccc tgctggtttt gagatggtta
      841 tcaataactt ctcattaggt attgcgggga tgctcctttg tctgctgggt tttgaagtta
      901 tcggcccggc ggtgttaatt gccaatactt tcgtcaaaga gtgtattgag gcgttggtac
      961 atgcgggtta tctgcctttg ctgtcagtca tcaatgaacc ggcgaaagtg cttttcctca
     1021 ataacgcgat agatcagggc gtctattacc cgttggggat gcaacaggct tcagttaacg
     1081 gtaaatccat cttctttatg gtggcgtcca accctgggcc tggcttagga ctgttgctgg
     1141 cgtttacttt gtttggtaaa gggatgagta aacgttctgc gcccggggcg atgattattc
     1201 acttcctcgg tgggatccac gaactgtatt tcccgtatgt gctgatgaag ccgctgacca
     1261 ttattgccat gattgcgggc ggtatgtctg gcacctggat gtttaactta ctggacggtg
     1321 gtctggtggc tggcccaagt ccggggtcta tctttgctta cctggcactg acgccgaaag
     1381 gctcgttcct ggcgacaatt gccggtgtta cggtaggtac cctggtgtcc tttgctatca
     1441 cttccgtaat actgaagatg gaaaaaacgg tggaaacgga gagcgaagat gagtttgctc
     1501 agtcagccaa tgcggttaag gcgatgaaac aagaaggtgc attctcgtta agcagggtta
     1561 agcgtatcgc ctttgtttgc gatgcgggga tgggctccag cgcgatgggc gcgaccacct
     1621 tccgtaaacg cctggaaaag gcggggctgg caatagaagt aaaacattac gcgatagaaa
     1681 acgtgcctgc ggatgcggat atcgttgtta ctcatgccag tctggaaggg cgcgtgaaat
     1741 gtgtgacgga taaaccgctg atactgatta ataactatat tggcgatcca aaactcgaca
     1801 ctttatttaa tcaattaacc gccgaacata aaaactgatt gccgaggtaa aaatgaaaac
     1861 caaagttgct gctatttatg gcaagcggga tgtccgtctg cgcgaatttg aactgccaga
     1921 aattaccgat aatgaactgc tggtgagtgt gatttctgac agcgtgtgtt tatccacctg
     1981 gaaagcggcg ctactcggta gtgaacataa acgcgtaccc gacgatttag aaaatcatcc
     2041 ggtcattacc gggcatgaat gcgccggggt tattgtcgaa gtgggtaaaa atctcaccga
     2101 caaatataaa aaaggccagc gttttgtatt gcaaccggca atggggttac caagcggata
     2161 ttcagcaggc tacagctacg aatattttgg cggtaacgcc acctatatga ttattcctga
     2221 aatagccatt aacctgggct gcgtattacc gtatcacggc tcttattttg ctgcggcgtc
     2281 gctggcagag cctatgtgct gcattattgg cgcttatcat gccaattatc acaccacgca
     2341 atatgtttat gagcatcgca tgggcgttaa acctggcggc aatattgcgc tgctggcatg
     2401 tgctggtccg atgggcattg gcgctatcga ttatgctatt aacggcggca tacaaccgtc
     2461 gcgggtggtg gtggtcgata tcgacgacaa acgcctggcg caggtacaaa agctgctgcc
     2521 ggtggaactg gcggccagta aaggcattga gctggtgtat atgaatacca aagggatgag
     2581 cgatccggtc cagacgctgc gggcgctgac ggatgatgcc ggatttgatg acgtatttgt
     2641 ctatgcagcg gtgccttcag tcgtcgagat ggctgatgaa ttactggcgg aagatggctg
     2701 cctgaacttc tttgccgggc cgacggataa aaacttcaaa gtgccgttta atttctacaa
     2761 cgtccattac aacagcacgc atgtcgtcgg tacgtccggc ggttcaacgg acgacatgaa
     2821 agaggcgatt gcgcttagcg ccacagggca gttgcagccg tcgtttatgg tgacccatat
     2881 cggtgggctg gatgcggtgc ctgataccgt gctcaatctg ccggatatcc ccggcggtaa
     2941 aaaactcatt tataacggcg tcaccatgcc gctcactgcc attgccgatt ttgccgaaaa
     3001 aggcaaaacc gatccgctgt ttaaagagct ggcgcggctg gttgaggaaa cgcacggcat
     3061 ctggaatgag caggccgaga aatatctgct ggcgcaattt ggcgttgata tcggggaggc
     3121 cgcgcaatga tgtccctggc gtggccatta tttcgcgtta cggaacaggc agcgttggct
     3181 gcctggccgc aaaccggatg tggcgacaaa aataaaattg atggcctggc ggtcaccgcg
     3241 atgcgccagg cattaaacga cgtcgctttt cgtgggcgag tggttatcgg cgagggggaa
     3301 cgatatcctc tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg gtatccaaca
     3361 gacggcgaat ggggtgcgga cgcagacatt actgatcggc ggcgcggacc aaacgtgtaa
     3421 tataatagac tccctgcatt gatggtgatc tatcggaaga ccaatggcga cgctgacaga
     3481 agatgatgtg cttgagcaac tggatgcaca ggacaattta ttttcattta tgaaaactgc
     3541 gcattctatt ttgctccagg ggatacgcca gtttctgccg tcgctgtttg tcgataacga
     3601 tgaagagatc gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc cgcttgacga
     3661 tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa atggataaat ggctgtacgc
     3721 cgatatcacg catttttccc agttttggca ttacctgaac gaacaggatg aaacgcccgg
     3781 atttgccgat gacatgacct gggattttat cagcaacgtc aatagcataa cctgcaatgc
     3841 gacgctctat gacgcgttga aagcgatgaa gttcgccgat ttcgccgtct ggtcagaggc
     3901 gcgttttagc aggatggtca aaacggcgct gacgctggca gtaacgacca ccttaaagga
     3961 attaacgccg tgaaaattga attaacggtg aatgggctga agatacaggc acagtaccag
     4021 aacgaagaga ttgaaaatgt acataaaccg ttgctgcaca tgttggcggc cttacagacg
     4081 gtaaatccgc agcggcgtac ggtggttttt ctttgcgctc cgccggggac aggtaaatct
     4141 accctaacca ccttctggga atatctcgca caacaagacc cggaactgcc cgctattcag
     4201 acactgccga tggatggttt tcaccattac aatagctggc tggatgcgca tcaattgcgc
     4261 cccttcaaag gcgcaccaga gacattcaac gttgcgaaac tggcggaaaa tctgtgccgg
     4321 gttgtggaag gggattgtac gtggccgcag tacgatcgac aaaagcatga tcctgttgaa
     4381 gatgcgttgc acgttaccgc accactcgtc atcgtcgaag gaaactggct attactggat
     4441 gatgaaaagt ggcaggagtt ggcatctttc tgcgattttt ctatttttat tcatgcacct
     4501 gctcaaattc tgcgggagcg cttgattagt cgtaaaattg cgggaggatt atcgcggcaa
     4561 gaagccgaag ctttttatgc ccgaaccgat ggtcccaatg tcgagcgtgt gctggtgaat
     4621 agtcgtcagg ccaatctgat attagagatg accgaagaag gcagatatca cttcaaaagc
     4681 tgacaattta ttccagttat tgcaggggta ttattacgcc agcctggaat aaagccccct
     4741 acaaaagggg ataaaagtgt gatgtaagtc agataaatgt cttcttcggc tggacaatca
     4801 ttccttttat tccacgtttc gcttatccta gctgaagcgt ttcagtcgat taaatgttcg
     4861 acaattaacc aatcagtcgc agtttgcgac aggtaaggtt tccccggacg atttgctgga
     4921 ttactctgtc tggcaaattt gctcagggaa aaccttgcag gagatctatg accgtacgcg
     4981 tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt tcgtgctttg tatgaatccg
     5041 gacgccgggc ggaaattacc gtggtggcaa tcaacgaact ggcggatgct gcgggcatgg
     5101 cgcatttgtt gaaatatgac accagccatg gccgttttgc atgggaagta cgacaggaac
     5161 gcgatcaact ttttgttggt gatgacgcca tccgtgtatt gcatgaacgt tcactgcaat
     5221 cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga ctgcaccggc gtatatggct
     5281 cccgcgagca tggcgaagcg catattgccg ccggggccaa aaaagtgctc ttttcacatc
     5341 caggcagtaa cgatctcgac gcgaccgttg tttacggcgt caatcaggat caacttcgtg
     5401 cggaacaccg catcgtttct aacgcttcct gtaccacgaa ttgcataatt cccgtcatca
     5461 aattgttaga tgatgcgtac ggtattgagt ccggcactgt gaccacaatt cactccgcca
     5521 tgcacgatca acaggttatt gatgcatacc atcctgacct gcgtcgcacc cgggctgcca
     5581 gccagtcgat cattccggtc gatactaaac tggccgccgg tatcacacga ttttttccgc
     5641 aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc aaccataaat gtgacggcaa
     5701 tcgatttaag cgtgacggtg aagaaacctg taaaagccaa tgaagtcaac ctgttgctgc
     5761 aaaaagcagc acaaggtgca tttcatggta tagttgacta tacggaattg ccgttggtct
     5821 ctgtagattt taaccacgat ccgcacagtg ccattgtcga tggcacccaa acccgggtta
     5881 gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa cgaatggggc tttgctaacc
     5941 gaatgctcga cacgacgtta gctatggcta ctgttgcttt caggtaagac gcaagcagcg
     6001 tctgcaaaac ttttagaatc aacgagagga ttcaccatgt ctgtaattaa gatgaccgat
     6061 ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc tgaacgtacc agtaaaagac
     6121 gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc cgaccatcga acttgccctg
     6181 aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc gtcctaccga aggcgagtac
     6241 aacgaagaat tctctctgct gccggttgtt aactacctga aagacaaact gtctaacccg
     6301 gttcgtctgg ttaaagatta cctcgacggc gttgacgttg ctgaaggtga actggttgtt
     6361 ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg acgaaaccct gtccaaaaaa
     6421 tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg gtactgctca ccgcgcgcag
     6481 gcttctactc acggtatcgg taaattcgct gacgttgcgt gcgcaggccc gctgctggca
     6541 gctgaactgg acgcgctggg taaagcactg aaagaacctg ctcgcccgat ggtggctatc
     6601 gttggtggtt ctaaagtatc taccaaactg accgttctgg actccctgtc taaaatcgct
     6661 gaccagctga ttgttggtgg tggtatcgct aacaccttta tcgcggcaca aggccacgat
     6721 gtgggtaaat ccctgtacga agctgatctg gttgacgaag caaaacgtct gctgaccacc
     6781 tgcaacatcc cggttccgtc tgatgttcgt gtagcaactg agttctctga aactgcaccg
     6841 gctaccctga aatctgttaa cgatgtgaaa gctgacgagc agatcctgga tatcggtgat
     6901 gcttccgctc aggaactggc tgaaatcctg aagaatgcga aaaccatttt gtggaacggt
     6961 ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta ctgaaatcgt ggctaatgct
     7021 atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg acactctggc agcaatcgac
     7081 ctgttcggca ttgctgacaa aatctcctac atctccactg gcggcggtgc attcctcgaa
     7141 ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg aagagcgcgc taagaagtaa
     7201 aaaatcacag ggcagggaaa cctgcccttg ttgcagcgcg cttttaaagc acgcactttt
     7261 tcatactcta aataattcga gttgcaggaa ggcgacaaga gagtgaatcc catgagctta
     7321 cataagtaag tgactggggt gagcgaacgc agacgcagca catgcaactt gaagtatgac
     7381 gagtataagg cccgacgata caggacaaga gacatgtcta agatttttga tttcgtaaaa
     7441 cctggcgtaa tcactggtga tgacgtacag aaagttttcc aggtagcaaa agaaaacaac
     7501 ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca tcaacgccgt gctggaaacc
     7561 gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca acggtggtgc ttcctttatc
     7621 gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg ctatcctggg cgcgatctct
     7681 ggtgcgcatc acgttcacca gatggctgaa cattatggtg ttccggttat cctgcacacc
     7741 gaccactgcg cgaagaaact gctgccgtgg atcgacggtc tgctggacgc gggtgaaaaa
     7801 cacttcgcag ctaccggcaa gccgctgttc tcttctcaca tgatcgacct gtctgaagaa
     7861 tctctgcaag agaacatcga aatctgctct aaatacctgg agcgcatgtc caaaatcggc
     7921 atgactctgg aaatcgaact gggttgcacc ggtggtgaag aagacggcgt ggacaacagc
     7981 cacatggacg cttctgcact gtacacccag ccggaagacg ttgattatgc gtataccgag
     8041 ctgagcaaaa tcagcccgcg tttcaccatc gcagcgtcct tcggtaacgt acacggtgtt
     8101 tacaagccgg gtaacgtggt tctgactccg accatcctgc gcgactctca ggaatatgtt
     8161 tctaagaaac acaacctgcc gcacaacagc ctgaacttcg tattccacgg tggttccggt
     8221 tctactgctc aggaaatcaa agactccgta agctacggcg tagtgaaaat gaacatcgat
     8281 accgataccc aatgggcaac ctgggaaggc gttctgaact actacaaagc gaacgaagct
     8341 tatttgcagg gtcagctggg taacccgaaa ggcgaagatc agccgaacaa gaaatactac
     8401 gatccgcgcg tatggctgcg tgccggtcag acttcgatga tcgctcgtct ggaaaaagca
     8461 ttccaggaac tgaacgcgat cgacgttctg taagtcattc ctttctgctt atctcaaggc
     8521 ccgctctgcg ggtctttttt tcgccaaaag caaggtgatg cagatgagaa atctgtgatc
     8581 tatttggcaa aattatgctt tattgtttac ccttgtcaga ctgcccgcca taaggcggcg
     8641 gagtgtattt ctccattttg agtcagttga aaaggaatat tgaatggaag atttgaatgt
     8701 tgtcgatagc ataaacggcg cgggaagctg gctggtggct aaccaggcgc tgctgctaag
     8761 ttatgcagta aacatcgtgg cggcactcgc gatcatcatt gttggtttga ttatcgcgcg
     8821 gatgatttcc aacgcggtga atcgcctgat gatctcccgt aaaatcgatg ccactgttgc
     8881 tgattttctc tctgcattag tccgttacgg tattatcgcc tttacgctaa tcgctgcact
     8941 ggggcgcgtg ggcgtacaaa cagcgtcagt cattgctgta ctcggtgccg caggcttagc
     9001 ggttggtctg gctttgcagg ggtcactgtc taacctggcc gctggcgtgt tacttgttat
     9061 gttccgcccg ttccgtgccg gagaatatgt tgatctcggc ggcgtagccg gtactgtgct
     9121 gagtgtgcag attttctcca ccaccatgcg tactgcagac ggtaaaatta tcgttattcc
     9181 gaacggtaaa attattgccg gaaatattat taacttctct cgcgagccag ttcgccgtaa
     9241 cgaatttatt attggcgtgg cgtatgattc cgatatcgat caggttaagc agatcctgac
     9301 cgatattatc cagtctgaag atcgcatttt gaaagatcgc gaaatgactg tgcgcctgaa
     9361 cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc tggagcaaca gcggcgatct
     9421 gcaaaacgtg tactgggatg tgctggagcg tattaaacgt gaatttgatg ccgccggtat
     9481 cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg gtgaaagaag acaaagctgc
     9541 gtaatcaacg ctgaggccag ataatactct ctggcctctc tcttattagt ttttctgatt
     9601 gccaattaat attatcaatt tccgctaata acaatcccgc gatatagtct ctgcatcaga
     9661 tacttaattc ggaatatcca acgtgttttc ttattacttt caaggtcttg cacttggggc
     9721 ggctatgatc ctaccgctcg gtccacaaaa tgcttttgtg atgaatcagg gcattcgtcg
     9781 tcagtaccac attatgattg ccttactttg cgctatcagc gatttggtcc tgatttgcgc
     9841 cgggattttt ggtggcagcg cgttattgat gcagtcgccg tggttgctgg cgctggtcac
     9901 ctggggcggc gtagccttct tgctgtggta tggttttggc gcttttaaaa cagcgatgag
     9961 cagtaatatt gagttagcca gtgccgaagt cctgaagcaa ggcagatgga aaattatcgc
    10021 caccatgctg gcagtgacct ggctgaatcc gcatgtttac ctggatactt ttgttgtact
    10081 gggcagtctt ggcgggcaac ttgatgtgga accaaaacgt tggtttgcgc tcgggacaat
    10141 tagcgcctct ttcctgtggt tctttggtct ggctattctc gcagcctggc tggcaccgcg
    10201 cctgcgcacg gcaaaatcac agcgcattat caatctggta gtgggatgtg ttatgtggtt
    10261 tattgccttg cagctggcga gagacggtat tgttcatgca caagccttgc tcagttaggc
    10321 gctatctgat ggaaaaataa aacagaggcg ctaagcttgc ctccagaggt cctgaatttt
    10381 aggcaagtca attaacacgg agagactaac gtgaagttca aagttatcgc cctggcggca
    10441 ttaatgggta ttagcgggat ggcagcgcag gctaacgaat tgccggatgg accgcatatt
    10501 gtcacctccg gtacggcaag cgtggatgcg gtgccagaca ttgccactct tgcgattgaa
    10561 gtcaacgtgg ccgcgaagga tgccgctact gccaagaaac aggcggatga gcgcgtcgca
    10621 caatacattt ccttccttga actcaatcag atcgcgaaaa aagatatcag ctcagcgaac
    10681 ttacgcaccc agccagatta tgattatcag gatggtaaaa gtatcctgaa aggctaccgt
    10741 gctgtgagaa cggtggaagt cacgctccgt cagttagaca aactgaattc cttgctggat
    10801 ggcgcgctga aggcgggtct taacgaaatt cgttctgtat cgctgggcgt ggcgcagccg
    10861 gatgcctata aagacaaagc gcgtaaggca gcgattgata acgcgattca tcaggcgcag
    10921 gaactggcga acggctttca tcgtaaactg gggccggtat atagtgtgcg ctaccatgtt
    10981 tccaactatc agcccagccc aatggtgcgg atgatgaaag ccgatgccgc gccggtgtcc
    11041 gcccaggaaa cttacgagca ggccgctatt cagtttgatg atcaggtcga tgtggtcttc
    11101 cagttagaac ctgtggatca acaacccgct aaaacacctg cagcacaata atatttgtag
    11161 cccgacgtat tcgtcgggct aatgccttta cccgatattg cctccttttt ctgtaatttt
    11221 ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt ttcatcaagg ataggctgac
    11281 tgaaacggtt aagcggacaa ggatgcgggg gagggggtat ggacattttt atttcgaaaa
    11341 agatgcgcaa tttcatttta ttagcgcaaa ccaataatat tgcccgggcg gcagaaaaaa
    11401 tccatatgac agcttcgcca tttggtaaaa gcattgccgc gctggaagaa caaattggct
    11461 atacgctatt tacccgcaaa gataataata tcagcctcaa taaagccgga caggaactgt
    11521 atcaaaaact gttcccggtt tatcaacggc tttctgcgat tgataatgaa atccataact
    11581 cagggcgtcg ttcacgagaa attgtgattg gcatagataa tacttatccg acaattattt
    11641 tcgatcagtt gattagtctc ggagataagt atgaaggcgt gaccgcccag ccagttgagt
    11701 tcagcgaaaa tggcgttatc gataatcttt ttgaccgtca gttagatttt attatctccc
    11761 cacagcatgt atctgcacga gttcaggaac tggaaaacct gactatcagt gaactaccgc
    11821 cgctgcgatt gggctttctc gtttctcgtc gttatgaaga gcgacaagag caagagttgc
    11881 ttcaggagct accctggcta caaatgcgct ttcagaaccg ggctaacttc gaagcaatga
    11941 ttgatgcgaa tatgcgtcct tgtgggatca atcccaccat tatttatcgt ccctacagtt
    12001 ttatggccaa aatcagcgcc gtggagcgcg gacattttct gacggtgatc ccgcattttg
    12061 cctggcgact ggtgaatccg gcaacgctga aatattttga tgcgccgcac aggccgatgt
    12121 atatgcagga atatctttat tcaatcagaa atcatcgtta taccgccacg atgcttcagc
    12181 atattgctga agatcgtgac gggacgagtc attaacccag catcgagccg gttgcaatta
    12241 aattacggtg taagtcgaag acgtggctaa gatcgtggtg aatatgtccg ccgggcgcat
    12301 tttccagata gcgatgcaga taatcccgat acataggatg tgcacaatta tcaatgatag
    12361 tgcgggcgcg ttgaagcggg gaaagaccac gcagatcggc gatgccttgt tcggtgatga
    12421 tcactttgac gctgtgttcg ctgtgatcaa catggctgca cattggcacg acggttgaga
    12481 tcttgccttc tttagcaatc gacggggcca taaagatcga cagatacgcg ttgcgttcaa
    12541 aatcaccgct gccaccgatg ccgttcatca gatcgacccc ggctacgtgc gttgagttgg
    12601 catgcccgta aatatcaaac tccaggccga cgttcagagc gatgacgccc agacgacgga
    12661 tgatttccgg gttattggaa atctcctgcg gacgcaacac aatgcggctg gcaaagtaat
    12721 ccatattgtc gtaaatcttg cgcagggaat cggccgagat agtcaggctg gaggcgctgg
    12781 ccccgctgat tttgcccgtt tccagtaaat gcaccaccga ttcctgtagc acttccgaat
    12841 acatcataaa cggaggaatt tccggatttt cccccagacg cgccattacc gcattattga
    12901 tgttgcctac gccactttgc agcggcagaa attccggcgg aatacgccca tgcgccattt
    12961 cctgcaacaa gaacgtgacc acgttatcgg caatctgctg gcacatggga ttttgcttat
    13021 ccagcatatt accggcgtca ggcaagttgg tttccacgac ggcgacaatc tttttcggat
    13081 cgatttgcac atagcgggta ccgacgcgat ccattgcatg gaagatcgac acgctattgc
    13141 gccgcggtgg cgcgctagga atcacaatat ctgccagttc tgcaacgcgc ggatcgtgat
    13201 agtgattgag ttcaatgatc actttcttcg cccgcagcag ccaggtcggc gcattaccga
    13261 tcccgctggt taaccagact cgaccatccg gtgccagtgc cgatgcttca atgacggcaa
    13321 catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc cacttcgctc aaatgcaggt
    13381 caacgaaact caccgcgccc tgattgatct ttttacgtaa accggacgat gtttgatatg
    13441 gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc gtcagcggcg gcgctgattg
    13501 acgcccccgt cagcaggcga atttgatacg gctttttggc ctcatgctgt tcgttagctc
    13561 tgcgggcaat cgcgttgggt aaggctttcg gcgaacccgc tggggtaaag ccgctaaatg
    13621 ccaccatgtc gttatgctgg ataatttctg ccgcttcatc ggcggtcatc cttgtccact
    13681 gagtttccat tttaactcct tcgttcgcag ggattaatga ccaacgaaat taggtttacg
    13741 tttttcgagg aaagcgttca tcccttcctg gtaatcttcg ctgtcataaa ccgcgcggcg
    13801 catcccctga atacgttcaa attcatcgga gttcatggtg tgcgcttcgc ccagtacacg
    13861 cagctcttct ttgataacgg caatggctaa cggcgctttc tcagagatgt ggtgcgccat
    13921 ttgtaaggtg aaatcttcca gttcttccac ttccacaaca tggttgagga tgccgacagc
    13981 cagcgcgcgc tgggctgtga ttggcgaagc ggtaaaaatc agctctttga caatgtggaa
    14041 gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc cgaggtttac
    14101 aggtgtcatt gagaaggttg aggtactggc ggcgatgatc agatcggaac tcatgatcat
    14161 ttcaaatgcg ccaccccaga cactaccttc caccatcgaa atgatcggtt tcgggaattt
    14221 ttggatcatg cgggtgattt gacgcaacgg atcatcatag gagagcggat cgcgaccgcc
    14281 agacggcagt tcgtgaatat cgtgacctgc ggagaagact ttggaaccac tcggcgcgcg
    14341 caaaatgata cagcgaattt ccggccggtt gagatcgctt aatgcctgca taagatcatc
    14401 aataaagact ttacttaagg cattaagttt gcggccatag ttaaactcaa tgaccgccac
    14461 tttgttgata gtgacaacgt tcacatactg ataagacata aaaattcctt taatcaaaat
    14521 attgcgtctg gataaattca ctgagctgcc gcaggccggt gcgcggtgag agcgtattgt
    14581 ttttgactgc taaaagcgtc tggcggtaat agcgatcgaa atcttcattc gcgaacaggc
    14641 gattcagtac ttcttcttcg gtctgcttac gcagccattc caccgattgt tgttgccgca
    14701 cttgttgtaa tcgaccactg gcagttagcg cggttttgaa gtcgatgatg gcgtgccaga
    14761 tctcatcgat tccacgtttt tccagtgcgc tacaagtcag aacccgtggc tgccattcgt
    14821 cgtatttacg tcgcagaata tgcagggcac tctcgtacat atgccgggca atggcgacat
    14881 tggtatggtt atcgccatcg tctttgttga taacgatcag atctgccact tccatcagcc
    14941 cctttttaat gccctgcaga tcatcgccgc caccggcaat ttgcaacgag ataaaacagt
    15001 ccaccatgcg ggcgacttcc gtttctgact gcccgacgcc aaccgtttcg acaatcacta
    15061 cgtcataacc cgccgcttcg cataacagca ttaattcccg cgctcgctga ctggcaccgc
    15121 ccagatggcc ggaggagggt accgggcgaa taaacgccgc ctcggcacgc gcaaggtcat
    15181 tcatgcgggt tttatccccg agaatgctac cgccagtgac cgggctgctg ggatcgaccg
    15241 caataaccgc aacctttaat ccctcacgaa tcaacaacat gccaaaggct tcaagaaagg
    15301 tacttttccc cgcgccgggt gtgccggtaa cgcccagtcg cagggcgtta ccacagaacg
    15361 gcataatggc gtcaagcagc tgtgtactta gcgcctgatg acgcgggtga cggctttcca
    15421 ccaacgtcat ggcctgggcg agcgtggcac gctcaccctg acgtaagcgg cgaatacttt
    15481 ctgctagctt ggcttcatta atcatgatgc tggcttatca gattcagtac gtcgcgcacg
    15541 ctgtcgagca taggtgtacc tggaccataa atcgccgcca cgccgcgctc ttgcaggaag
    15601 gcgtaatcct gcggcggaat gacgccaccc gcgaccacgc agatatcttt gcgtccccat
    15661 tttttcagcg cttcgaccag ttccgggatc agcgttttat gaccggcagc cagtgaggat
    15721 gcgcccacta cgtgaacgtc gttttctacg gccaggcggg cgatctcttc aggtgtagag
    15781 aacatcgggc ttaaatctac gtcgaaaccg agatcggaat aggcgctggc gatcactttc
    15841 gcgccgcgat cgtgcccatc ctggcccatt ttggcgatca gaatgcgcgg gcgacgacca
    15901 ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat cgaactcggc ggccgatttc
    15961 tcagactgat gatagctttg cgcaatcacg ccggtaacac actggcttgg caccagataa
    16021 cggtcgaaag cggcttccag cgcatcggaa atttcaccca gggtggcgcg aacgcgagca
    16081 gcattaacag cggcagccag caggttttcg ttatgctgtg cggcgtgagt cagggcgttc
    16141 aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag cgaagcaatt
    16201 tgctcgttac gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc ttcgtgatcc
    16261 agtttgtact tgttgacacc aacgatgaca cgcttgccct ggtcgatcag cgactgttcg
    16321 cgcgctgagg cctcttcgat cattcgtttt ggcagacctg cttcgatcgc tttcgccatg
    16381 ccaccggctt cgtcgatctg ttggataata gctctggctt gtttgacgat ttgatcggtc
    16441 agcgactcaa tgtaatagga tccggccagt ggatcgacgg tgcggcagag ttctgattct
    16501 tcctgaatga tgatctgggt attgcgggca atgcgtgctg agaaatcggt aggcaaacca
    16561 agcgcttcat caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt tgcagccagt
    16621 gcttcaatgg tggtgcggat aacgttgtta tacggatcct gttcagtcag gctccagcct
    16681 gaggtctggc agtgggtacg cagcgccagt gatttcggat cctgtgcgcc aaatccactg
    16741 accgcttcgc tccataaata acgtgccgca cgcaacatgg cgacgttcat aaacagatcc
    16801 atgccaatgc cgaagaagaa cgacagacga ggagcgaagt catcaatttt cagcccggcg
    16861 gagattgctg ctttgatgta ctcaatccca tcggcgagcg taaatgctac ctgctgcacg
    16921 cagttggcac ccgcttcacc catgtggtaa ccgctgatac tgatggtatt aaatcgcggc
    16981 atgttgccgg aacaccaggc gatgatgtct gcgataatac gcattgacgg ttttggcggg
    17041 taaatatagg tgttgcggca aagatactct ttgaggatat cgttttgaat ggtgccggtc
    17101 agtttatcgg gtgtaacacc ttgctcttct gcggcgacaa tataaaacgc cagtactggt
    17161 agtactgcgc cattcatggt catcgaaacc gacattttat ccagcgggat ctggtcgaac
    17221 aggactttca tatcttccac ggtgtcgata gcaacgcccg ctttgccgac gtcgcccgcc
    17281 acgcgtgggt tatcggagtc gtagcctcgg tgggtggcaa ggtcaaacgc aacggaaaga
    17341 cctttttgcc cggcggccag gttacggcga taaaaagcgt tggactcttt tgctgttgaa
    17401 aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc acgcgggcca
    17461 cgaacgtagg gaggcaaacc aggaagggta cctgtcacct ccagattatc gagatcggct
    17521 tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag cgagtcgaca
    17581 gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg caccttagac
    17641 attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg tgagcttttt
    17701 acgtgggttt gtcgccagca aaaaaaatgc attcagacgt cgttttttgc cgcctgagat
    17761 ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa gtgaggataa cgcctgatat
    17821 gtgctatcag gcattatttg atggattaat cctgacgaag gactttgtga ccataatcga
    17881 gcaacgcatc agtgacttta cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga
    17941 gcatccgtcg ttgaaatagc cctggcgtta agtcaatcag ttcaccgctg gccagctctt
    18001 tctcgatttg caggtgcggg atcatacagc aggtggtgcc ctgacgagca agttgcacga
    18061 acgcttccga agaattaacg atatggcagg gtacgctgcc aggaggcaga tcgaagtttt
    18121 gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa agcgaccaca ggtgctttca
    18181 ataatgccga acgcgttacg ccgttaggga aatatttttc ggcaaacggt tttgagctga
    18241 cgaacagata gtcgagcgca ccgagcttat cgacaaggca actcggcagc gcctgatgtt
    18301 gaatactcac cgcgccgacc acttcgccgc ggcgcagacg ttcctgagtg cgggtttcat
    18361 cttctacctg caagttgagg cggataggcg aatcagctaa cacaggagcc agcgccggaa
    18421 gcaaccacgt cgccagactg tcagcgttga ccgccagtga aagcagcagc ggagttgaac
    18481 cggtttgttc atcgcccagc cactcttctt ccagtaactc cacctggcgt agcagtgcca
    18541 gcagtttttg cccttgttct gtcgggcgcg gtggtacggt acgcaccaac agcggctgcc
    18601 cgaacatatt ttccagttgc ttaatgcgct gtgagacggc tgattgtgta atgcacagct
    18661 tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc
    18721 tgtagtccgg gcgtttcatt gctgttgtct gctcctggta aatgcttttt cctgcactat
    18781 gacacaattt tgtgtcaggt tgcatatacc gaagtcgttg gcgggtaaca tttgtatcag
    18841 tggttaacgc gcagcttaaa agatcaggga tttgcgctaa tgcatttcgc tcagcgagtt
    18901 cgcgccctgg ttgtgttgaa tggcgtggcg ttattgcctc tgttcgcatc gtggagcggc
    18961 atacccagac cgttgtatgc tttatttgcg gggcgaaagg ggatgcccgc cattgcgcga
    19021 tattttatgg atgagttaac cacccggctt gccaacgggg tctgaatcgc tttttttgta
    19081 tataatgcgt gtgaaatttc ataccacagg cgaaacgatc atgacgcagg atgaattgaa
    19141 aaaagcagta ggatgggcgg cacttcagta tgttcagccc ggcaccattg ttggtgtagg
    19201 tacaggttcc accgccgcac actttattga cgcgctcggc acgatgaaag gccagattga
    19261 aggggccgtt tccagttcag atgcttccac tgaaaaactg aaaagcctcg gcattcacgt
    19321 ttttgatctc aacgaagtcg acagccttgg catctacgtt gatggcgcag atgaaatcaa
    19381 cggccacatg caaatgatca aaggcggcgg cgcggcgctg acccgtgaaa aaatcattgc
    19441 ttcggttgcg gaaaaattta tctgtattgc cgacgcctcc aagcaggttg atattctggg
    19501 caaattcccg ctgccagtag aagttatccc gatggcacgt agtgcagtgg cgcgccagct
    19561 ggtgaaactg ggcggtcgtc cggaataccg tcagggcgtg gtgaccgata acggcaacgt
    19621 gatcctcgac gtccacggca tggaaatcct tgacccgata gcgatggaaa acgccataaa
    19681 tgcgattcct ggcgtggtga ctgttggctt gtttgctaac cgtggcgcgg acgttgcgct
    19741 gattggcaca cctgacggtg tcaaaaccat tgtgaaatga tctgacgggg gaacctcccc
    19801 cgttaaaaaa attctcttca ttaaatttgg tgacatgtgt cacgttttta cctggctatt
    19861 gtcgattgct ctaaataaac cctctaaacc agcatattca tccaagaaat acctttgcgt
    19921 gatatttcct caatatcgtg acgcaaacgt tcatattgcc gcaatattat tttttgatat
    19981 gttgaaaggc ggatgcaaat ccgcacacaa catttcaaaa gacaggattg ggtaaatggc
    20041 aaaggtatcg ctggagaaag acaagattaa gtttctgctg gtagaaggcg tgcaccaaaa
    20101 ggcgctggaa agccttcgtg cagcgggtta caccaacatc gaatttcaca aaggcgcgct
    20161 ggatgatgaa caattaaaag aatccatccg cgatgcccac ttcatcggcc tgcgatcccg
    20221 tacccatctg gctgaagacg tgatcaacgc cgcagaaaaa ctggtcgcta ttggctgttt
    20281 ctgtatcgga acaaaccagg ttgatctgga tgcggcggca aaacgcggga tcccggtatt
    20341 taacgcaccg ttctcaaata cgcgctctgt tgcggagctg gtgattggcg aactgctgct
    20401 gctattgcgc ggcgtgccgg aagccaatgc taaagcgcat cgtggcgtgt ggaacaaact
    20461 ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt atcatcggct acggtcatat
    20521 tggtacgcaa ttgggcattc tggctgaatc gctgggaatg tatgtttact tttatgatat
    20581 tgaaaacaaa ctgccgctgg gcaacgccac tcaggtacag catctttctg acctgctgaa
    20641 tatgagcgat gtggtgagtc tgcatgtacc agagaatccg tccaccaaaa atatgatggg
    20701 cgcgaaagag atttcgctaa tgaaacccgg ctcgctgctg attaatgctt cgcgcggtac
    20761 tgtggtggat attccagcgc tgtgtgacgc gctggcgagc aaacatctgg caggggcggc
    20821 aatcgacgta ttcccgacgg aaccggcgac caatagcgat ccatttacct ctccgctgtg
    20881 tgaattcgac aatgtccttc tgacgccaca cattggtggt tcgactcagg aagcgcagga
    20941 gaatatcggt ctggaagttg cgggtaaatt gatcaagtat tctgataatg gttcaacgct
    21001 ctctgcggtg aacttcccgg aagtctcgct gccactgcac ggtggtcgtc gtctgatgca
    21061 catccacgaa aaccgtccgg gtgtgctgac tgcgctcaac aaaatttttg ccgagcaggg
    21121 cgtcaacatc gccgcgcaat atctgcaaac ttccgcccag atgggttatg tagttattga
    21181 tattgaagcc gacgaagacg ttgccgaaaa agcgctgcag gcaatgaaag ctattccggg
    21241 taccattcgc gcccgtctgc tgtactaaat ccccttatct gaaaatcaac gggcaggtca
    21301 ctgacttgcc cgttttttta tcccttctcc acaccgttgc cgcattgcgc attctacttt
    21361 gcgagtctgc tccagaaaat aaatcaaagt cagatcaatg cgttgtgttt atttaatggg
    21421 gatttacgat ggcagggcag catggggctg taacggtaaa gccctcaccg aagcgagggc
    21481 ttgaaggaga agggttatga tgcgacttgt catcatactg attgtactgt tactcataag
    21541 tttcccagct tattaacagt cagtctcagg ggaggagtca tcctccctta cccttactca
    21601 ctaaattagg ttaaagaatc aacgatgtca atcagggcga tgcaggtgtg tcgccctcac
    21661 cactcccaga ctttcgacgg tgtaaccacc gcaggaagag ggatatccca ctcttcaacg
    21721 gggagttttt ccaccaactg acaatcatgc gcataaccca ccggttgcat tttatagtgc
    21781 tgccagtttt gtaaggtccg atcataaaaa ccaccgccca ttcccaggcg ctgaccgtac
    21841 tcatcaaagg cgaccagcgg tgtgatcaac acgtctaatc gggaaagggg tagcacgtca
    21901 cgcacatcca gtttgggctc atggatcttc aacctgttca tcaccagttc gctttgcgga
    21961 tggtaattca ggaacagcaa attaccggca ctgaagggat gcaaaactgg aagatatacg
    22021 cgcttaccgg cgcgccagag ttgttctatg agtggcaggg tgtcgagttc gccatcaaaa
    22081 gagaggaata cagcgaccgt atgtgccatc accaccgggg gataagtcat catccgggta
    22141 gcggcttgtt gacccatttc ctgctgttgt tccggcgtta acgcacgacg acgttgccga
    22201 atcattttgc ggatttcttg tcgggataat gtcagtggga gttctggtag ttgcgtcatg
    22261 gctggtgcca gataagaagg gaatctccga gatgccgccg caggctgtaa cccttgaacc
    22321 cttggttcaa ggtgaatgtg tcgtcgcagt tttaaggctt ctcggacgga ccgagcatgc
    22381 tcaccaaccg cggagcgcca cattcttgtg gtatgaaata tcggctcagg ggactggccc
    22441 gcttgcgaac atctcagaga aattttgtct tcacggttac tctaccacag taaaccgaaa
    22501 agtgtcattc aaagttttgg ttagtttttt cggtgatgcg accttgttca aataacgctt
    22561 gttctatggt ctgctgcagc atccgaatac gctgttccat acttgccgca tagtcacgag
    22621 tcttcgcttt ttcttgcgct aactcatagc tgatattcaa tgcggcaatg aagaccaact
    22681 gttcagtatt tgtgactcta gtgcgttctt tcagatcttg caaccgttgg ttcagatcgt
    22741 ccgctgcctg attcaacgca tccctttggt caggcgggca gttcacacgc agtgaacggc
    22801 caaaaatttg gatatcgacg ggttgtgcag acatgccacc ttcctgctga ttgactgcgc
    22861 tgccttcgtc tttagaccca tggtctgcga aggggcgaca ctatagctac cctgatgaga
    22921 agagacaagc ccttttctgg tccaccaggg cccaaagtgg tagcatatca tgaatattcc
    22981 tccctttgac gacgaatgct tatgtctata cagaacgaaa tgcctggtta caacgaaatg
    23041 aaccagtatc tgaaccaaca agggacgggt ctgacccctg ctgagatgca tggtttaatc
    23101 agcgggatga tatgtggcgg taacgatgac agctcatggc tgccgctact tcacgacctg
    23161 acgaacgaag gcatggcttt cggtcatgag ctggcacagg cactgcgtaa aatgcactct
    23221 gccaccagcg atgccctgca ggatgacggc ttcctttttc agctttatct gcctgatggc
    23281 gatgatgtca gcgttttcga tcgggctgat gcgctggcag gttgggtcaa tcacttcctg
    23341 cttggtcttg gcgttacgca accgaagctg gacaaagtga ccggagaaac cggtgaagcc
    23401 atcgacgatc tgcgtaacat cgcgcagctg ggctacgacg aagacgaaga tcaggaagag
    23461 cttgaaatgt cgcttgaaga gatcatcgaa tacgttcgtg ttgccgcgct gttatgccac
    23521 gacaccttta ctcatccgca accgaccgcg ccagaagtac aaaaaccgac tctacactaa
    23581 aaacaaaaaa cgtaaggaga gtgttatgag tgagatatcc cggcaagagt ttcagcgtcg
    23641 ccgtcaggcc ctggtggagc aaatgcaacc cggcagcgcc gcgctgattt ttgctgcacc
    23701 agaagtaaca cgtagcgccg acagcgaata cccctatcgt cagaacagtg acttctggta
    23761 cttcaccggc tttaacgaac cggaagcggt gctggtgctg attaaaagcg acgacactca
    23821 taaccacagc gttttgttta accgcgttcg cgacctgacg gcagagatct ggtttggtcg
    23881 tcgcttaggc caggatgccg cgccagagaa actgggcgtt gaccgcgcac tggcattcag
    23941 cgaaatcaat cagcaacttt atcaactgct taatggtctg gatgtggttt atcacgctca
    24001 gggcgaatat acctatgctg atgaaatcgt gaacagcgcg ctggaaaagc tgcgtaaagg
    24061 ctcgcgacag aatctcactg ccccggctac gatgatcgac tggcgtcctg ttgttcatga
    24121 aatgcgcctg ttcaaatcac cagaagagat tgccgtactt cgccgcgcgg gtgaaatcac
    24181 cgcactggcg catacccggg cgatggaaaa atgccgtccg gggatgttcg agtaccatct
    24241 ggaaggcgaa attcaccacg agtttaaccg ccacggtgcg cgttaccctt cctataacac
    24301 cattgttggc agcggtgaaa acggctgcat tttgcactac accgaaaacg agtgtgaact
    24361 gcgtgacggt gatctggtgt tgattgacgc gggttgtgaa tacaaaggtt acgctggcga
    24421 cattacccgc accttcccgg tcaacggcaa attcactcag gctcagcgtg aaatctatga
    24481 cattgtgctg gagtctcttg aaaccagcct gcgcctgtat cgtccgggaa cttccatgca
    24541 ggaagtcact ggtgaagtgg tgcgtatcat ggttagcggc ctggtaaaac tcggcatcct
    24601 gaagggtgat gttgatgaac tgatcgctca gaacgcccat cgtcctttct ttatgcatgg
    24661 ccttagccac tggttaggac tggatgtcca tgacgttggc gtttatggtc aggatcgctc
    24721 gcgcattctg gaaccgggga tggtactgac cgtagagcct ggtctgtata tcgcgccgga
    24781 tgcggatgtg ccagaacaat atcgcggtat cggcattcgt attgaagacg acattgtgat
    24841 taccgaaacc ggcaacgaaa acctcaccgc cagcgtggtg aaaaagccgg aagaaatcga
    24901 agcgttgatg gctgctgcga gaaagcaatg agcgtaatca tcgtcggtgg cggcatggcg
    24961 ggcgcgacgc tggcgctggc tatttcccgg ttaagtcacg gggcgctgcc ggtacatttg
    25021 attgaagcga ctgcgccaga gtcacatgct catccgggct ttgatggacg agcgatagcg
    25081 ctggcggcgg gtacctgtca gcaactggcg cgcatcggcg tctggcaatc tctggcggat
    25141 tgcgcaaccg ccatcactac cgtgcatgtc agcgatcgtg gtcatgccgg attcgtcacc
    25201 ctcgccgcag aagattacca actggcggcg ctggggcagg ttgtcgaatt gcataatgtc
    25261 gggcaacggc tgtttgcatt gctgcgcaaa gcacctggcg taacgctgca ttgtcctgat
    25321 cgcgtggcta acgttgcccg tactcagagt cacgttgaag tgacgctgga gagtggcgag
    25381 acgctgacgg gctgcgtgct ggtagccgct gatggcaccc attcagcgtt agccaccgtg
    25441 tgcggcgttg actggcagca ggagccttac gaacaactgg ctgtgattgc caacgttgcg
    25501 acttccgttg cgcatgaagg gcgcgctttt gaacgcttta cacaatatgg cccgctggcg
    25561 atgttgccga tgtctgacgg acgctgttcg ctggtctggt gtcatccact ggaacggcgc
    25621 gaagaggtac tgtcttggag cgacgagaag ttttgccgtg aactccagtc ggcctttggt
    25681 tggcgacttg ggaaaattac ccacgctggt aaacgcagtg cttatccgct ggcgttaacc
    25741 cgcgccgcca gacctattac ccatcgtacc gtgctggtgg gcaatgcggc gcaaactctg
    25801 cacccgattg ccgggcaagg gttcaacctc ggtatgcgag atgtcatgag ccttgcggaa
    25861 accctgactc aggcgcagga gcgcggagaa gacatgggtg attacggcgt attgtgccgt
    25921 tatcagcagc gtcgacagcg cgatcgcgaa gcaaccatcg gcgtcacgga cagccttgta
    25981 catctttttg ccaaccgttg ggggccgctg gttgtcgggc gcaacatcgg gctgatggca
    26041 atggaattat tcaccccagc acgcgatgtg ctggcgcagc gcaccctcgg gtgggtggcg
    26101 cgttgaaggc ggttttaagg aataaacaat gcaaagtgtt gatgtagcca ttgttggcgg
    26161 cggcatggtg gggctggcgg ttgcctgcgg cttgcagggt agcggcttac gcgttgccgt
    26221 actggagcag cgcgtaccgg aacctctggc ggcggatgca ccaccacaac tgcgcgtttc
    26281 ggctatcaat gccgccagcg aaaaattact cacccgtctt ggtgtctggc aggacattct
    26341 ctctcgtagg gccagttgtt atcacggtat ggaagtgtgg gacaaagaca gttttggtca
    26401 catttcgttt gacgatcaaa gcatgggcta tagccatctt gggcatatcg ttgaaaactc
    26461 ggtgattcac tacgcgctgt ggaacaaagc gcagcagtcg tcagatatca ctctgttggc
    26521 ccccgcagaa ttacagcagg tcgcctgggg agaaaatgaa accttcctga cgttgaaaga
    26581 tggcagtatg ttaacggcgc gtctggtgat tggcgcggac ggcgctaatt cctggttgcg
    26641 caacaaagcc gatattccgc tgactttctg ggattatcag catcacgcgc tggtagcgac
    26701 cattcgcacg gaagaaccgc atgatgcggt ggcgcggcag gtttttcatg gcgaaggcat
    26761 tctggccttt ttgccgctta gcgatccgca tctttgctcg attgtctggt cactgtcgcc
    26821 agaggaagcg cagcggatgc agcaggcagg cgaagacgaa tttaatcgcg cgttaaatat
    26881 cgcttttgat aatcgcctgg gcttatgcaa ggttgaaagt gagcgcctgg tgttcccgct
    26941 aacggggcgt tatgcgcgcc agtttgccgc acaccgtctg gcgctggtgg gcgacgccgc
    27001 gcatactatt cacccgctgg cggggcaggg ggtaaatctc ggctttatgg atgctgcaga
    27061 gctgattgct gaactgaaac ggttgcatcg tcagggtaaa gacatcgggc agtacattta
    27121 cctgcgtcgc tatgagcgta gccgtaagca cagtgcggcg ctgatgctgg ctggtatgca
    27181 gggattccgc gatctgtttt ccggtaccaa tccggcgaaa aaattgctgc gtgatattgg
    27241 tctgaaactg gctgatacgc ttcctggcgt taagccacaa cttattcgtc aggcaatggg
    27301 attaaacgat ttgcctgaat ggctgcgtta aatatttctc ttctgctgtt tatttgatac
    27361 ccatcacact ttcatctccc ggttttttcg ccgggagatt ttcctcattt gaaataaact
    27421 aatttaacct ccgttttcgc attatatttt ctaatgttat ggatttttga tttagtgttt
    27481 tttgacattt ttttagcgct taatattgtc ttattcaaat taactttctc atcacatcat
    27541 ctttgtatag aaactgatgt gttttttggt tttttattct gttgcgattt ttgcattttt
    27601 taaccataag ctaatgtgat gatcaatttt accttatggt taacagtctg tttcggtggt
    27661 aagttcaggc aaaagagaac gattgcgttg gggaccggga gtggctccga tgctgggttt
    27721 cgtggtgata atttcgccat ggaaaagttg tcagccccgc ttattcaatg aggacaagat
    27781 ggcacaacag actcctttgt acgaacaaca cacgctttgc ggcgctcgca tggtggattt
    27841 ccacggctgg atgatgccgc tgcattacgg ttcgcaaatc gacgaacatc atgcggtacg
    27901 taccgatgcc ggaatgtttg atgtgtcaca tatgaccatc gtcgatcttc gcggcagccg
    27961 cactcgggag tttctgcgtt atctgctggc gaacgatgtg gcgaaactca ccaaaagcgg
    28021 caaagccctt tactcgggga tgttgaatgc ctctggcggt gtgatagatg acctcatcgt
    28081 ctactacttt actgaagatt tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga
    28141 cctctcctgg attacccaac acgctgaacc tttcggcatc gaaattaccg ttcgtgatga
    28201 cctttccatg atcgccgtac aagggccgaa tgcgcaggca aaagctgcca cactgtttaa
    28261 tgacgcccag cgtcaggcgg tggaagggat gaaaccgttc tttggcgtgc aggcgggcga
    28321 tctgtttatt gccaccactg gttataccgg tgaagcgggc tatgaaattg cgctgcccaa
    28381 tgaaaaagcg gcggatttct ggcgcgcgct ggtggaagcg ggcgttaagc catgcggcct
    28441 gggcgcgcgt gacacgctgc gtctggaagc gggtatgaat ctttatagtc aggagatgga
    28501 cgaaaccatc tctcctttag ccgccaatat gggctggact atcgcctggg aaccggcaga
    28561 tcgtgacttc atcggtcgtg aagccctgga agtgcagcgt gagcatggca cagaaaaact
    28621 ggttggtctg gtgatgaccg aaaaaggcgt gctgcgtaat gaactgccgg tacgctttac
    28681 cgatgcgcag ggcaaccagc atgaaggcat tatcaccagc ggtactttct ccccgacgct
    28741 gggttacagc attgcgctgg cgcgcgtgcc ggaaggtatt ggtgaaacag cgattgtaca
    28801 aattcgcaac cgtgaaatgc cggttaaagt gacgaaacct gtttttgtgc gtaacggtaa
    28861 agccgtcgcg tgatttactt ttttggagat tgattgatga gcaacgtacc agcagaactg
    28921 aaatacagca aagaacacga atggctgcgt aaagaagccg gcggcactta caccgttggt
    28981 atcaccgaac atgcccagga gctgttaggc gatatggtgt ttgttgacct gccggaagtg
    29041 ggcgcaacgg ttagcgcggg cgatgactgc gcggttgccg aatcagtaaa agcggcgtca
    29101 gacatttatg cgccagtaag cggtgaaatc gtggcggtaa acgacgcact gagcgattcc
    29161 ccggaactgg tgaacagcga accgtatgca ggcggctgga tctttaaaat caaagccagc
    29221 gatgaaagcg aactggaatc actgctggat gcgaccgcat acgaagcatt gttagaagac
    29281 gagtaacggc tttattcctc ttctgcggga gaggatcagg gtgaggaact tttacgcctc
    29341 accctcactc tcttcgcaag gagagaggtt cacaattcac tgcacgtttc aggaaccatc
    29401 gctcatgaca cagacgttaa gccagcttga aaacagcggc gcttttattg aacgccatat
    29461 cggaccggac gccgcgcaac agcaagaaat gctgaatgcc gttggcgcac aatcgttaaa
    29521 cgcgctgacc ggccagattg tgccgaaaga tattcagctt gcgaccccgc cgcaggttgg
    29581 cgcaccggcg accgaatacg ccgcgctggc ggaactcaag gctattgcca gtcgcaataa
    29641 acgcttcacg tcttacatcg gcatgggtta caccgccgtg cagctaccgc cggttattct
    29701 gcgtaacatg ctggaaaatc cgggctggta taccgcgtat actccgtatc aacctgaagt
    29761 ctcccagggc cgccttgaag cactgctcaa cttccagcag gtaacgctgg atttgaccgg
    29821 gctggatatg gcctctgcct ctcttctgga cgaggccact gctgccgccg aagcaatggc
    29881 gatggcgaaa cgcgtcagca aactgaaaaa tgccaaccgc ttcttcgttg cttctgacgt
    29941 tcatccgcaa acgctcgatg tggtccgtac tcgtgctgaa acctttggtt ttgaagtgat
    30001 tgtcgatgac gcgcaaaaag tgctcgacca tcaggacgtc ttcggcgtgc tgttacagca
    30061 ggttggcaca accggtgaaa tccacgacta cactgcgctc atcagcgaac tgaaatcacg
    30121 caaaattgtg gtcagtgtcg ccgccgatat aatggcgctg gtgctgttaa ctgcgccggg
    30181 taaacagggc gcggatattg tctttggttc ggcgcaacgc ttcggtgtgc cgatgggcta
    30241 cggtggccca cacgcagcat tctttgcggc gaaagatgaa tacaaacgct caatgccggg
    30301 ccgtattatc ggtgtatcga aagatgcagc tggcaatacc gctctgcgca tggcgatgca
    30361 gactcgcgag caacatattc gccgtgagaa agcgaactcc aacatttgta cttcccaggt
    30421 actgctggca aacatcgcca gcctgtatgc cgtttatcac ggcccggttg gcctgaaacg
    30481 tatcgctaac cgcatccacc gtctgaccga tatcctggcg gcgggcctgc aacaaaaagg
    30541 tctgaaactg cgtcatgcgc actatttcga cactttgtgt gtggaagtgg cagacaaggc
    30601 gggcgtactg gcgcgtgccg aagcggctga aatcaacctg cgtagcgata tcctgaacgc
    30661 ggttgggatc acccttgatg aaaccaccac gcgtgaaaac gtgatgcagc ttttcagcgt
    30721 actgctgggg gataaccacg gcctggacat cgacacgctg gacaaagacg tggctcacga
    30781 cagccgctct attcagcctg cgatgctgcg cgacgacgaa atcctcaccc atccggtgtt
    30841 taatcgctac cacagcgaaa ccgaaatgat gcgctatatg cactcgctgg agcgtaaaga
    30901 tctggcgctg aatcaggcga tgatcccgct gggttcctgc accatgaaac tgaacgccgc
    30961 cgccgagatg atcccaatca cctggccgga atttgccgaa ctgcacccgt tctgcccgcc
    31021 ggagcaggcc gaaggttatc agcagatgat tgcgcagctg gctgactggc tggtgaaact
    31081 gaccggttac gacgccgttt gtatgcagcc gaactctggc gcacagggcg aatacgcggg
    31141 cctgctggcg attcgtcatt atcatgaaag ccgcaacgaa gggcatcgtg atatctgcct
    31201 gatcccggct tctgcgcacg gaactaaccc cgcttctgca catatggcag gaatgcaggt
    31261 ggtggttgtg gcgtgtgata aaaacggcaa catcgatctg actgatctgc gcgcgaaagc
    31321 ggaacaggcg ggcgataacc tctcctgtat catggtgacc tatccttcca cccacggcgt
    31381 gtatgaagaa acgatccgtg aagtgtgtga agtcgtgcat cagttcggcg gtcaggttta
    31441 ccttgatggc gcgaacatga acgcccaggt cggcatcacc tcaccgggct ttattggcgc
    31501 ggacgtctcg caccttaacc tgcataaaac tttctgcatt ccgcacggtg gcggcggccc
    31561 tggtatggga ccgatcggcg tgaaagcgca tctggcaccg tttgtgccag gtcatagcgt
    31621 ggtacaaatt gaaggcatgt taacccgtca gggcgcggtt tctgcggcac cgttcggtag
    31681 cgcatccatt ctgccaatca gctggatgta catccgcatg atgggcgcag aagggctgaa
    31741 aaaagcaagc caggtggcaa ttcttaacgc caactatatt gccagccgcc tgcaggatgc
    31801 cttcccggtg ctgtataccg gtcgcgacgg tcgcgtggcg cacgaatgta ttctcgatat
    31861 tcgcccgctg aaagaagaaa ccggcatcag cgaactggat attgccaagc gtctgatcga
    31921 ctacggtttc cacgcgccga ccatgtcgtt cccggtggcg ggtacgctga tggttgaacc
    31981 gactgaatct gaaagcaaag tggaactgga tcgctttatc gacgcgatgc tggctatccg
    32041 cgcagaaatt gaccaggtga aagccggtgt ctggccgctg gaagataacc cgctggtgaa
    32101 cgcgccgcac attcagagcg aactggtcgc cgagtgggcg catccgtaca gccgtgaagt
    32161 tgcggtattc ccggcaggcg tggcagacaa atactggccg acagtgaaac gtctggatga
    32221 tgtttacggc gaccgtaacc tgttctgctc ctgcgtaccg attagcgaat accagtaatt
    32281 cactgattcg actatcttct aaaggcgctt cggcgccttt ttagcttgtt aataaagtgc
    32341 gctttgtgtg tcggatgaag cgtaaatata aactcctgga gttcaatata tgacaggagc
    32401 gccaggcctg atgagcgggg cgcatcaggc acatcgggta attaaagctt ctcgccgttg
    32461 ctggcaatca cttctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca
    32521 gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta
    32581 gtgaacgaca cgcaatcgat gcagccccac ggtgtgtagc ccatcagatc cacgccatcg
    32641 taagtcaccg cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtca
    32701 tcgttgatgc tgccatcttc ttccactttg tcataagcgc caaaaccgtt ttcgacaata
    32761 aacagcggct tctgataacg ctcatacagt tcgcaaagtg catagcgcag acccacagga
    32821 tcaatctgcc agccccagtc agacgcttta acatacgggt ttggtacgct gccttcaaaa
    32881 ccagagatcg catcgccggt gccgccttcg gccttcaccg cgttggtcat gtaatagctg
    32941 aaaccgagat aatcgcaggt gccttcgcgc agcacatcga gatcgccgtc ttccattttg
    33001 atgttaaatc cgcgacgctc ccactcgtgc aacacatagg acgggtaata gccacgcagt
    33061 tgcacatcgg taaagacgta gcgttcgcgc atcgactcct gggcgaacat cacatcgtcc
    33121 ggattacagg agtaagggta aagcggcacc atcgccagca tacagccgac tttcatttcc
    33181 gggttaatgc gacgcgcggc tttcaccgcc agggcgctgg cgacaaactg gtgatgcagc
    33241 acctggtaca tcgtctcttc cgggttttca tgctcggtat acaccacgcc ggagcagcag
    33301 taaccgaaca gcggtgcacg ccagttacgc tggttgttaa tttcgttgaa ggtcatccag
    33361 tatttgactt tatgcttata gcgttcaaat accacttctg cgaaacggac aaagaaatca
    33421 accactttac ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag
    33481 tgggagaggg tgatcaccgg ttcgatgttg tatttcagca gttcatcgaa catatcatcg
    33541 tagaacttca gcccttcttc gtttggctga gcttcatcgc cttttggaaa aatgcgcgtc
    33601 caggcgatgg atgtacgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc
    33661 ttgtaatgac cataaaaatc aacggcttca tggttcggat agtattttcc tggcaacact
    33721 tctttggtga tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc
    33781 ggccctttgc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac tgcgccgccc
    33841 cataagaaat ctttcggtaa ggtgagtttt ttcactatca taggctccag atgagtcaat
    33901 ttttacagga aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga
    33961 tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa
    34021 aacgggtgat gtgcttcgtg tcgggcgttt tgaagacgac ggttattttt gcacgattga
    34081 agtcaccgca acctcaaccg taacgctgga tacactgacg gaaaaacatg cagagcagga
    34141 aaatatgacc ctgactgatc tgaaaaaggt cattgccgat atctatcccg gccagacaca
    34201 gttttatgtg attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt
    34261 cgcatttaat ttcatgattt ttcggagatt agtctcaaat cagaatttat tttagctaac
    34321 aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt
    34381 ctccggagta agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg
    34441 aaattgccaa cagcgtcagt cacggcattg ggttggtgtt tggtatcgtt gggctggtgt
    34501 tgctactggt tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc
    34561 tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc
    34621 atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctattgattg
    34681 ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga
    34741 tgattgttat ctggagcctg gcattgctgg ggattctgtt caaactgacc atcgcgcacc
    34801 gattcaaaat tttatctctg gtgacctatc tggcgatggg ctggctgtcg ctggtggtga
    34861 tttatgaaat ggcaattaag cttgcggcgg gcagcgttac cttactggct gtaggcggcg
    34921 tggtttactc gctcggggtg attttctacg tctgcaaacg cattccctac aaccatgcca
    34981 tctggcacgg ctttgtgctc ggcggcagcg tgtgccactt tctggcgatc tatttatata
    35041 ttgggcaggc gtaatgggtg tgcctttgta ggcctgaaaa gacacaccct tgtcgcatca
    35101 ggcgataaat gcatgatgcg acacagagat ttactcttcg agtgaatacg gcagcggctc
    35161 gatatgcaat gtattcgcat cgtcacgcac gcggaagatg ctgtccggtt ccatatcgtt
    35221 attcatgacg acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcacggt
    35281 tccggtacga cgccagttct cgcccatttt cagctctaag tcttcaccgg cttccggcag
    35341 tcggctggcg ctacccgtca gcaaccagag cgcgcgtttg ttggcaccac ggaatttagc
    35401 tcgcgccacc atctcttgtc cggtatagca gcctttttta aagctaatgc cgcccagcgc
    35461 ctggaggttg gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa
    35521 acccgcttca atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg
    35581 cagtttatcg gtcagcatat tggcagtggc ttcatcggtt acgatcagga aacgttctgc
    35641 cgggtgttca aaccatagca aagtggtcgc gccttcttta actacctgtt tttctttcga
    35701 aggcagttca ctaaagatat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag
    35761 cagcacacgc tcgtcgtctg gcgcgatggt cactttagag aacaccgcat attttttcag
    35821 ttcagtcagc tgcggttcac gcacgctgcg ccgttcaatc cacgcaaagc catcgccgtc
    35881 gcggaacaga cgtaaattgc tccacatttt gcctttggcg tcgcaatggg cggcgagcag
    35941 gtgctgatct tctgtcatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc
    36001 gctgtccgcg ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg
    36061 taaacgggca gaagccgtag gctgacgggg aggaaaaggt gtaaaagcca taatagagtc
    36121 ctgagttgct taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt
    36181 gctttcacta cgcgatctgt gccttctgat atgctttgcg aggcgacttc caaaaaaatg
    36241 aagcccttga attgttgcga attaatttcg cgatcgcgat tgtgtttact gatattcccg
    36301 caaaagaagt cgaaaaacac gttacaatag ccgggtactg gattttcgtg agacaggaag
    36361 aagatggaca ttaacaacaa agcccgcatt cattgggcat gccgccgtgg tatgcgcgaa
    36421 ctcgatattt caatcatgcc gtttttcgaa catgagtacg acagcttaag cgatgacgaa
    36481 aaacgcatct ttattcgtct gctggaatgc gacgacccgg acctgtttaa ctggctgatg
    36541 aatcacggta aaccggccga tgcagaactg gaaatgatgg tccgactcat ccagacacgg
    36601 aaccgggaac gtggtcctgt ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct
    36661 ttccttgctg attcatgggc tggttgccgc tgttatttta ctcatgccct ggccgctcag
    36721 ttacaccccg ttatggatgg tgttgctttc gctggtggtg tttgattgcg ttcgcagcca
    36781 gcgccgcatt aatgctcgcc agggggaaat tcgcctgttg atggacgggc gtttgcgttg
    36841 gcaagggcag gagtggagca tcgtcaaagc gccgtggatg attaagagcg gcatgatgct
    36901 gcgtttacgt tctgacagcg gtaaacggca acatttatgg ctggcagccg acagcatgga
    36961 cgaagctgaa tggcgggatt tacggcggat tttgttgcaa caagagacgc aaagataaag
    37021 atgacggcaa agcatatgcg ctaagccgtc agtgacatca ggcgtaatgc tctgccattt
    37081 cgttgaggat ttgctcgcac cagctctgaa tacgctcgtc gctaaggtca tactggttag
    37141 tttcatccag cgccagaccc acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg
    37201 taaattcata tccttccgtt ggccagtagc cgacgaactt cacgcctttg gtcgagagtt
    37261 tgtcatgcag cataccgagc gcatcgagga accactcgcc gtatcccagt tgatcgccaa
    37321 gcccatacag cgcaacaatt ttaccttcga ggttcaggtc gtcgagctga tcccagacgg
    37381 cttcccagtc ttcctggatt tcgccaaaat cccaggtcgg gatacccaga atgagcacat
    37441 cgtactgctc cattaatttc ggggagtcgt ccttgaggtt atgtaaggtc accagttctg
    37501 ggccgataat atcgcggatt ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt
    37561 aaaaaagacc catattcata acgtaagctt ctcgattctg ctgtggcttt tgcggctagt
    37621 gtatcagaat cgctttagtc acggcacaat aaggcataat ggatcaaatg agtgaccata
    37681 aggggcgcaa gtgaaacagg atctggcacg catcgagcag tttcttgatg ctctgtggct
    37741 ggaaaaaaat ctggctgaaa atacgttgaa cgcttaccgt cgcgatctgt caatgatggt
    37801 ggagtggttg catcaccgtg ggttgacgct ggcgacggcg caaagtgacg atttgcaggc
    37861 attactggca gaacggctgg agggcgggta taaagccacc agctcagcgc gtttgctgag
    37921 tgcagtgcgc cgattgttcc agtatcttta tcgcgaaaag tttcgtgaag atgatcccag
    37981 tgcgcatctc gcttcaccga aattgcccca gcgtttgcca aaagatttaa gcgaagcgca
    38041 ggtcgaacgt ttattacagg caccattaat tgatcagcca ctggagctac gcgataaagc
    38101 catgcttgaa gtgttgtatg ctaccggact gcgtgtctct gaactggtcg ggctgacaat
    38161 gagtgatatc agcctgcgtc agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg
    38221 cctggtgccg ttaggtgaag aggcggttta ctggctggaa acctatctgg aacatgggcg
    38281 tccgtggctg ttgaatggtg tgtcaattga cgtgttgttt cccagccagc gtgcgcagca
    38341 gatgacgcga cagaccttct ggcaccgcat taaacattat gccgtgctgg cgggtatcga
    38401 cagcgaaaag ctgtcaccgc atgtgttgcg tcacgctttt gccactcact tattaaatca
    38461 tggtgcggat ttacgcgtgg tgcagatgct actgggccac agcgatctct ccaccacgca
    38521 aatttatacg catgtcgcta ccgagcgtct gcgacaactt catcaacagc atcacccgcg
    38581 ggcgtgatgt ctgaaaagaa caggaaaatt tatgaagaaa ggttttatgt tatttacctt
    38641 gttggcagcg ttttcaggct ttgttcaggc tgatgacgcg gcaattcaac aaacgttagc
    38701 caaaatgggc atcaaaagca gcgatattca gcccgcgccc gtagctggca tgaagacagt
    38761 tctgactaac agcggtgtgt tgtacatcac cgatgatggt aaacatatca ttcaggggcc
    38821 aatgtatgac gttagtggca cggctccggt caatgtcacc aataagatgc tgttaaagca
    38881 gttgaatgcg cttgaaaaag agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat
    38941 caccgtgttt actgatatta cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga
    39001 ctataacgcg ctggggatca ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag
    39061 cgatgcagag aaagaaatga aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga
    39121 tgatgtgatg gcaggtaaaa gcgtcgcacc agccagctgc gacgtggata ttgccgacca
    39181 ttacgcactt ggcgtccagc ttggcgttag cggtactccg gcagttgtgc tgagcaatgg
    39241 cacacttgtt ccgggttacc agccgccgaa agacatgaaa gaattcctcg acgaacacca
    39301 aaaaatgacc agcggtaaat aattcgcgtg aaacaacaga tacaacttcg tcgccgagaa
    39361 gtcgatgaaa cggccgactt gcccgctgaa ttgcctccct tgctgcgccg tttatatgcc
    39421 agccggggtg tgcgtagcgc gcaggaactg gaacgcagtg ttaaaggcat gctgccctgg
    39481 cagcaactga gcggcgtcga aaaggccgtt gagatccttt acaacgcttt tcgcgaaggc
    39541 acgcggatta ttgtggtcgg tgatttcgac gccgacggtg ccaccagcac ggctctaagc
    39601 gtgctggcga tgcgctcgct tggttgcagc aatatcgact acttggttcc aaaccgtttc
    39661 gaagacggtt acggcttaag cccggaagtg gtcgatcagg cccatgcccg tggcgcgcag
    39721 ttaattgtca cggtggataa cggtatttcc tcccattcgg gcgttgaaca cgctcgctcg
    39781 ttgggcattc cggttattgt taccgatcac catttgccgg gcgaaacatt acccgcggcg
    39841 gaagcgatca ttaaccctaa cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc
    39901 gtgggtgtgg cgttttatct gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg
    39961 tttgatgagc gcggcgtcgc aattcctaac ctggcagaac tgctggatct ggtcgcgctg
    40021 ggaacagtgg cggacgtcgt gccgctggac gccaataacc gcatcctcac ctggcagggg
    40081 atgagtcgca tccgtgccgg aaagtgccgt ccagggatta aagcgctgct ggaagtggca
    40141 aaccgtgatg cactaaaact cgccgccagc gatttaggtt ttgcgctggg gccacgtctc
    40201 aatgctgccg ggcgactgga cgatatgtcc gtcggtgtgg cgctgttgct gtgcgacaac
    40261 atcggcgaag cgcgcgtgct ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag
    40321 atcgaacaag gaatgcagat tgaagccctg accctgtgcg agaaactgga gcgcagccgc
    40381 gacacgttac ccggcgggct ggcaatgtac caccccgaat ggcatcaggg cgttgtcggt
    40441 attctggctt cgcgcatcaa agagcgtttt caccgtccgg ttatcgcgtt tgcgccagca
    40501 ggtgacggta cgctgaaagg ttccggtcgc tccattcagg ggctgcatat gcgtgatgcg
    40561 ctggagcgat tagacacact ctaccccggc atgatgctca agtttggcgg ccatgcgatg
    40621 gcggcgggtt tgtcgctgga agaggataaa ttcgaactct ttcaacaacg gtttggcgag
    40681 ctggttaccg agtggctgga tccttcacta ttgcaaggcg aagtggtgtc agacggtccg
    40741 ttaagcccgg ccgaaatgac tatggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg
    40801 gggcagatgt tcccggagcc gctgtttgac ggtcatttcc gtctgctgca acagcggctg
    40861 gtgggcgagc gtcatttgaa agtcatggtc gagccggtcg gcggcggtcc gctgctggat
    40921 ggtattgctt ttaatgtcga taccgccctc tggccggata acggcgtgcg cgaagtgcaa
    40981 ctgacttaca agctcgatat caacgagttt cgcggcaacc gcagcctgca aattatcatc
    41041 gacaatatct ggccaattta gcgtcatctt ctctataaaa aagagcgtgg attgggtaca
    41101 atcccgctct tatcaccgca ttttgactag ctcaataaaa gaaatcagac catgtttgaa
    41161 attaatccgg taaataatcg cattcaggac ctcacggaac gctccgacgt tcttaggggg
    41221 tatctttgac tacgacgcca agaaagagcg tctggaagaa gtaaacgccg agctggaaca
    41281 gccggatgtc tggaacgaac ccgaacgcgc acaggccctg ggtaaagagc gttcttccct
    41341 cgaagccgtt gtcgacaccc tcgaccaaat gaaacagggg ctggaagatg tttctggtct
    41401 gctggaactg gctgtagaag ctgacgacga agaaaccttt aacgaagccg ttgctgaact
    41461 cgacgctctg gaagaaaaac tggcgcagct tgagttccgc cgtatgttct ctggcgaata
    41521 tgacagcgcc gactgctacc tcgatattca ggcggggtct ggcggtacgg aagcacagga
    41581 ctgggcgagc atgcttgagc gtatgtatct gcgctgggca gaatcgcgtg gtttcaaaac
    41641 tgaaatcatc gaagagtcgg aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat
    41701 ctccggcgat tacgcttacg gctggctgcg tacagaaact ggcgttcacc gcctggtgcg
    41761 taagagcccg tttgactccg gcggtcgtcg ccacacgtcg ttcagctccg cgtttgttta
    41821 cccggaagtt gatgacgata tcgatatcga aatcaatccg gcggatctgc gcatcgacgt
    41881 ttatcgcgcg tccggcgcgg gcggtcagca cgttaaccgt accgaatctg cggtgcgtat
    41941 tactcacatc ccaaccggga tcgtgaccca gtgccagaac gaccgttccc agcacaagaa
    42001 caaagaccag gccatgaagc agatgaaagc gaagctttat gaactggaga tgcagaagaa
    42061 aaatgccgag aagcaggcga tggaagacaa taaatccgac atcggctggg gcagccagat
    42121 tcgttcttat gtccttgatg actcccgcat taaagatctg cgcaccgggg tagaaacccg
    42181 caacacgcag gccgtgctgg acggcagcct ggatcaattt atcgaagcaa gtttgaaagc
    42241 agggttatga ggaaccaaca tgtctgaaca acacgcacag ggcgctgacg cggtagtcga
    42301 tcttaacaat gaactgaaaa cgcgtcgtga gaagctggcg aacctgcgcg agcaggggat
    42361 tgccttcccg aacgatttcc gtcgcgatca tacctctgac caattgcacg cagaattcga
    42421 cggtaaagag aacgaagaac tggaagcgct gaacatcgaa gttgccgttg ctggccgcat
    42481 gatgacccgt cgtattatgg gtaaagcgtc tttcgttacc ctgcaggatg tgggtggccg
    42541 cattcagctg tacgttgccc gtgacgatct gccggaaggc gtttacaacg agcagttcaa
    42601 aaaatgggac ctcggcgata tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg
    42661 cgaactgtct atccactgta ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc
    42721 ggataaattc cacggcttgc aggatcagga agcgcgctat cgtcagcgtt atctggatct
    42781 catctctaac gatgaatccc gcaacacctt taaagtgcgc tcgcagatcc tctctggtat
    42841 tcgccagttc atggtgaacc gcggctttat ggaagttgaa acgccgatga tgcaggtgat
    42901 ccctggcggt gccgctgcgc gtccgttcat cacccaccat aatgcgctgg atctcgacat
    42961 gtacctgcgt atcgcgccgg aactgtacct caagcgtctg gtggtcggtg gcttcgagcg
    43021 tgtattcgaa atcaaccgta acttccgtaa cgaaggtatt tccgtacgtc ataacccaga
    43081 gttcaccatg atggaactct atatggctta cgcagattac aaagatctga tcgagctgac
    43141 cgaatcgctg ttccgtactc tggcacagga tattctcggt aagacggaag tgacctacgg
    43201 cgacgtaacg ctggacttcg gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa
    43261 gaaatatcgc ccggaaaccg acatggcgga tctggacaac ttcgactctg cgaaagcgat
    43321 tgctgaatct atcggcatcc acgttgagaa gagctggggt ctgggccgta tcgttaccga
    43381 gatcttcgaa gaagtggcag aagcacatct gatccagccg accttcatta ctgaatatcc
    43441 ggcagaagtt tctcctctgg cgcgtcgtaa cgacgttaac ccggaaatca cagaccgctt
    43501 tgagttcttc attggtgggc gtgaaatcgg taacggcttt agcgagctga atgacgcgga
    43561 agatcaggcg cagcgcttcc tggatcaggt tgccgcgaaa gatgcaggtg acgacgaagc
    43621 gatgttctac gacgaagatt acgtcaccgc actggaacac ggcttaccgc cgacagcagg
    43681 tctgggaatt ggtatcgacc gtatggtaat gctgttcacc aacagtcata ccatccgcga
    43741 cgttattctg ttcccggcga tgcgtccggt gaaataagca ttacgttatg ctcacaaccc
    43801 cggcaaatgt cggggttttt taattgtgct gcgcgaaagc agacaacaat tttcttgctt
    43861 cactattggc tgcctgcatc accatccacg gactgaacgc ccacggcgtg gcatcaatac
    43921 cgtgtaatac atctgctaaa tcacaccatt gataatccat cacttcatca tcgttgatct
    43981 gtaacgcact ggtggtgcgt gcggcaaata ccggacacac ttcattttcc acaatgccat
    44041 tcggatcggt ggcgcgatag tagaagtcag gatagataga ttcaggaggc gtaatttcca
    44101 cgccaagctc ataacggcaa cggcggatca ccgcgtcttc gttgctttct cccagttgtg
    44161 ggtgcccaca aaccgagtta gtccacacgc caggccacgc ttttttgcta agggcgcggc
    44221 gggtaactaa taattgccct ttggcattaa acagccaact ggagaacgcg agatgtaaga
    44281 gagtgtctgc cgtgtgtgcg gcatactttt ccagcgtacc cgtgggaact ccctgtgcat
    44341 tcaataaaat gacgtgttcc gtttgcataa tttctcacat gtaattctaa tcactcatca
    44401 ttatagataa ttgaagagtg aaatgattaa ccaagtgagc gtttatcgac aaccgcccgt
    44461 tttgagcgga tgtcgacagg taaaaaccat ttaaaaaagt agcatcatat atttgaagcg
    44521 actttcaatg gccattgcaa cacagtcaga aatgacagga atagagagag taaaaagcaa
    44581 cagcagactg aatatagcgc gcattaaaag attcatatta ttctccatgc tcatttttct
    44641 tcagcttgcg catcagaagc cacatacgta ctgtcctgac ttttaaatct ttatcagaga
    44701 cggattcctt caccagatca gcatgatgct gatagccatt aaagaagacg ttcagcacaa
    44761 cggcgctaag ggttgcgagc ataatgccgc tatgcagcaa cggttgcagt acggccggta
    44821 acttagaaaa gaaatcgtga gagagcgtcg gagtcatgcc aacgccgaga ctgatcgcca
    44881 caatatagag gttgtaacgg ttggtggtgt agttacagcg cgacagaatt cgaatccctg
    44941 tcgccagtac catgccgaac atcaccagac cagcgccgcc cagcacaaat tgcggaatgg
    45001 aggcgaccag caccgccatt tttggcacca tgccaaacag gattaatata attcccgaag
    45061 aaatacacac ccagcggcta tgaacgcgcg tcacgctaac caggccaacg ttttgagaaa
    45121 atgacgtatg ggggaagctg ttaaacgttc cgcctatcat tgtccctacg ccatcgacac
    45181 gcagcccgcg aataatatcg tgtgaagaga gtttacgacc aactatttca cccagtgcca
    45241 ggaacatccc catcgactcg ataaacacga tgattaacac ggcagtcatg gtcagaatgg
    45301 aaacgggatc aaaaatcggc ataccaaacg acatcggggt gacaatcgca aaccacgagg
    45361 catcatgtag cccggataaa ttgacttcat tcatcatcca cgaaagtaaa aagccaaata
    45421 caatccccag taatacggcg acgttggaca taaatccttt cgcatagcga gtaatgagca
    45481 agataaaaat taagacggca aaggagatac ctaaataaac gggattacca tattgcggat
    45541 tccctttacc tcctgcggcc cagtcaatac ccacctgaat gatgctaagt ccgatagaag
    45601 taataaccac accggtaacc agtggcggga ataaaggcat caagcgaccg ataagtggcg
    45661 ctaataatgt ggtgataaaa cccgcggcga tagtggcacc aaatatcccc agcaggccga
    45721 tatccgggtt catcccaatg gctatcattg gtgttacagc ggcgaaggtc accgacataa
    45781 tcaccggcag gcggatcccc ataaagcggc cgataccgat acattgcaat aatgtgacga
    45841 tcccgcagca aaagagatcc gagctaatga gcatcgcaat agcttctttt gagaggccca
    45901 gtcggtcgcc aatcataaga ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt
    45961 gcagaccgag tatgatcagc tttcctggtg ataatatgcg gtcaacctca tcagttgggc
    46021 ggtcttgccc agaagatgag ggaagttggg aatctatggc gctcatcagt gtttctcctt
    46081 tcgcccgcca gagtaaaaag caaccgttta cgttcatgaa actgtctaaa tacatgctcg
    46141 tggagtgcca gttttgcgag gaaggggagt tagagacagg aattgcggag gtaatcacat
    46201 aatcgtggtt aaaccacgaa aatgagaaga gaacctgaca atggaattat ctgtaatttt
    46261 ctcatattga tatttcatta atcattctgg catgtcgcac tctcgcattt aatcgttttt
    46321 atctggatag cattcttttg atcggcaatg ttgaaataat gaaagctgca tagattgagg
    46381 ggcaataatt tgaactcagt cgcaaaatta taattaaatc ggccatgcta cctaatacat
    46441 gcccatttat tgcatcgcga aatttgagaa gacataactc attaaatgaa agggatgcaa
    46501 atgaataagt ttatcgctgc tgaagctgcg gaatgtatag gctgtcatgc ttgtgaaatt
    46561 gcctgtgcga tggcacataa tcaagaaaac tggccgctga gtcacagtga ctttcgaccg
    46621 cgtatccacg ttgtagggaa aggccaggct gcgaatccgg tggcctgcca tcactgcaac
    46681 aatgcccctt gcgttacggc ttgtccggtt aatgctctga ctttccagcc cgatagcgta
    46741 caactggacg agcaaaaatg tattggttgt aaaagatgcg caatcgcttg cccctttggc
    46801 gtcgttgaga tggtcgatac gattgcgcag aaatgcgacc tttgtaacca gcgcagttcc
    46861 ggcacgcaag cctgtattga agtctgccca acacaggcgt tacggctgat ggacgataaa
    46921 gggttgcagc agataaaggt ggcccgccag cgcaaaacgg cagcaggaaa agcgtcatca
    46981 gacgctcagc catctcgcag tgcagcgttg ctccccgtta actcgcgtaa aggcgcagat
    47041 aaaatttcag cgagtgaacg gaaaacccac tttggcgaaa tctattacgg gctggatcca
    47101 caacaagcga cttatgagag tgaccgctgt gtttattgcg ccgaaaaagc taactgcaac
    47161 tggcattgcc cgctgcataa cgctattccg gattacatcc gtctggtgca ggaaggaaag
    47221 attattgaag cggcagaact ttgccaccag accagttcct tgccagaaat ctgcggcagg
    47281 gtatgtccac aggaccgtct ttgtgaaggc gcatgtactt tgaaagatca ctctggcgca
    47341 gtctctatcg ggaatctgga acgctacatt accgataccg cgctggcgat gggctggcgt
    47401 cccgatgtca gcaaagttgt tccccgtatc gaaaaagtgg cggtgattgg cgcagggcct
    47461 gcagggttag ggtgtgctga tattttggcg cgcgcaggag ttcaggttga tgtctttgat
    47521 cgccatccag aaattggcgg tatgctgact tttggcatac ctcctttcaa actcgataaa
    47581 acggtattaa gccagcggcg agagatattc accgcaatgg gaatcgactt tcatcttaac
    47641 tgtgaaattg gccgcgatat tacctttagc gatttaactt ctgaatatga tgcagttttt
    47701 atcggcgtgg ggacttacgg gatgatgcga gcagatctgc cgcatgaaga tgcgcccggt
    47761 gtcattcagg cgttaccgtt cctgactgcc catacccgcc agctcatggg gttgccggag
    47821 tctgaagagt atccgctgac ggatgtggaa ggtaagcgag tcgtggtatt gggcggtggc
    47881 gatacgacca tggattgttt gcggacttcc atccgcctca atgccgccag cgtgacctgc
    47941 gcgtatcgtc gtgatgaagt cagtatgccg ggctcgcgca aagaggtggt caatgcgcgc
    48001 gaggaaggtg tcgagtttca gttcaatgtt cagccgcaat atatcgcttg tgacgaagat
    48061 gggcgtttaa ctgcggtggg cctgattcgt accgccatgg gtgagccggg gccggatggt
    48121 cgccgtcgtc ctcgtccggt agcgggttca gagtttgaat tgcccgccga tgttctcatt
    48181 atggcctttg gtttccaggc gcatgccatg ccgtggttgc agggcagcgg aattaaactc
    48241 gataaatggg gcctgattca aaccggtgac gtcgggtatt tacctaccca gacgcatctg
    48301 aaaaaagtct ttgctggtgg tgatgctgtt catggcgcgg atctggttgt cactgcaatg
    48361 gccgcaggaa ggcaggcggc gcgcgatatg ttaactctgt ttgatacgaa ggcatcatga
    48421 tgaaatcgtt aattatcgtt aatccggctg actgtattgg ctgccgtact tgtgaagtag
    48481 cctgtgtggt cgctcaccct tcagaacagg agttgaatgc cgatgtcttt ttaccccggc
    48541 tgaaggtaca gcgactggac agcatcagtg cgccggtgat gtgccatcag tgtgaaaacg
    48601 ccccttgtgt tagcgcttgc cccgtggggg cgctgacgat gggcgagcag gtggtgcaga
    48661 ccaattctgc ccgttgtatt ggctgtcaga gttgcgtcag tgcgtgcccg tttggaatga
    48721 tcaccattca gtcgttgccg ggggataccc ggcaacaaat cgtgaaatgc gatctctgtg
    48781 aacagcggga agaggggcca gcctgcgttg aatcttgccc aacacaggcg ctgcagttgc
    48841 ttactgaaag agaactcagg cgagtccgcc agcagcgtat tgttgccagc ggtgagaatc
    48901 ctctgtgaaa gaaatctccg cgctgaaagg cgcggaggat ctattagatt gcccaaccac
    48961 ccgcgtaaaa tgcgaccagt gcggcagtaa tgatcaccgt accaatattc agcttttgcc
    49021 attcgcgtgc aaagacgcgg cctacgacca gtgtcacaaa gccaagcata ataccggtaa
    49081 cgatattaca agtcagaacg atgaacacgg cacacaccag gccagccatt gcgtcaataa
    49141 aatcattgaa atccagcttc gagacgttac ttaacatcag caaacctacg tacatcagag
    49201 cgggggcagt ggcgtaacca gggatcaaaa atgacagcgg tgataagaac agaatcagca
    49261 ggaataacgc ccccactacg gttgcggtta aaccggtttt ccccccggcg gcggttcctg
    49321 ccgctgattc gatataaacc gccgcgggcg ctgcgcccac caggccggag aatattgaac
    49381 ttactgagtc actggtcagg gctttgccgc cgttgatgat ctggttgtct ttatccaaca
    49441 aattcgcctg accggcgacg gcacggatgg tgccagtggc gtcgaacact gcggtcatca
    49501 ccaatgccag tacactcgga agtacagttg gctggagtgc gcccataata tcgaggctga
    49561 aaatcagaga cttaccatct tcgccagtca ggcttggcat cgccaccaga ccgtggtact
    49621 tcaccgctgg gtcaaagatt aagccgatga tcgaaattgc aataatcacc aacaagatcc
    49681 cgccgggtac gcgacacttc tccagaccga agataaccgc cagccccagc aagctcatca
    49741 tcaccgggaa ggaggtaaac gcaccgagcg ccactggcaa gccttcaatc gggtttttga
    49801 taaccattcc cacaccgtta gcagcaatca gcagcagaaa caggccgata ccgatacctg
    49861 taccgtgagc aatccccatc ggcaaattac gtaagatcca ggtacgcaca ccggttacgg
    49921 aaatggcggt gaagatgacg cccatcagaa ataccgcgcc cagtgcgaca ggaacgctaa
    49981 tttgttgccc gagtaccaga ctgaatgcgg taaacgccgt caaggaaatc gcacaaccaa
    50041 tcgccattgg caaattggcc cacaacccca tcagcaacga gccgaagccc gcgaccagac
    50101 aggtggcaac aaacacagct gcgggaggaa aacctgcttt gcccagcatt cccggaacga
    50161 cgataacgga ataaaccatg gccagaaagg tcgttaagcc agccagtact tcctgacgaa
    50221 cggtactgcc acgagcggta attttaaaat aattatccag cgcgccctgt ggctttggtg
    50281 cgtccggtgt ttgtaggatg tctccagaca taatgatgtc ctcacgagga tgttaaagtt
    50341 ttattattag ttgcgttcgt acaccagacg accatcaacg taggtgcggt agatcgaacg
    50401 gtcatcgccc aacgtcatca tcacgaacaa tttgtcgact aaagaaacag agttgtcata
    50461 gcgcagctgc tgtagcggag tggcagtggg ttccatcacc acgaaatcag cctctttgcc
    50521 aggtaaaaag ttgccaatca aatcgtcaag gcccagagat ttcgctccgc cgagcgtggc
    50581 caggtaaaac gcttcatatg ccgagaggcg atagccttgt aattgcaata ccttgtaggc
    50641 ttcgttcagc gtttgcagca tgttaaaagt tgttccggca ccgatatccg ttcccatgcc
    50701 cactttgact ttcttctgcc atgctttttt caagttgaat aagccgctgc cgaggtaaag
    50761 gttggaggtc ggacagaaag caatgctgga tttggtttcg ctgagacgat cccactcttt
    50821 ttcttcgaga tggacgcagt gagcaaagac acagttttta ccggtcagac cgtactgatg
    50881 gtaaacatcc agataaccat catggtcagg ataaagcgat ttgacccagg caatttcatc
    50941 tttgttttca cagagatggg tatgtaccca cgtatccgga tattcttctt tcaggcgttg
    51001 cgccatcgcc atctgttcag gagatgaggt aggggcgaag cgtggcgtaa tcgcatacaa
    51061 cagacgacca tttttgtgcc agcgttcaat cagttctttg ctttggtgat agctgctttc
    51121 ggcagtgtcg agcagataat ccggtgcgtt tcggtccatc atcaccttac cggcaatcat
    51181 acgcatattg atatgactgg cggcttcaaa cagcgcatca acggattgtg gatgaacagt
    51241 gccaaacacc agcgccgtgg tggttccgtt acgtaaaagc tgcttgatga agaacgccga
    51301 catctcgcgg gcgtactcta aatcctcata acgacgttca gtagggaagg tgtgtttatt
    51361 caaccactcc agcaattgtt caccataggc ccccaccatt tcactttgcg gataatggat
    51421 atgtgtatcg acaaagcccg gtactatcag tttgccgcga tagtcgcgca cgcgaatggt
    51481 gtcaggaatt tgatgctttc cgtcttccca ttcgccaaac cattccactt ttccctgttt
    51541 aatgagtaat aaaccatcct caataaaccg cagcgcagag gcaatttctt ccgggttatc
    51601 gaccgtacgg gtgacatcaa taaaactgcc tcgtaccgct tttaacgtgt gttctcctga
    51661 catcattgac tcctttactg ttaatccatc tcttccgctg aggtaatacc aggcagaacg
    51721 ttttcctgtc ggtagccacc agggaggata atatttaata aaatcgcagt taacccgcca
    51781 gcacaaatag ggttttcaac taatacataa atagaggctg gcaatatttt aaaaatttcg
    51841 ggatcgtagg agacgccaag ccctaaacct aaagacgtgg cgacaataag tgtttctcgg
    51901 cgctttaatc cgttggtgat gatgatgcga atcccagcga tggcaatcat ggaaaacatc
    51961 aacgtcattg cgcctcccag aactgccgag ggaatggtcg tgaagaagcc gccaatcatc
    52021 ggaaataagc cgaggataac cagcattacc gcgatggttc gcccgacata acgtgaagcg
    52081 acgccagtca tctgaataac cccattattt tgcgcaaacg tggttaatgg taatgaaccg
    52141 acagcggagg cgataacaga aaccagacca tctgccagca cgccgccttt cagtcgggac
    52201 tgatactctt ccccctgaat ggggcggcgg gaaaccattg ccgtggcggt gatatcgccg
    52261 actgcttcca gcacgctaag cagataaatc gtgccaacca ccaggaactg atggaagcta
    52321 aaactaaagc cgtatttgaa cggatgcggg atggtgatta acggcaaatt gcgcatactg
    52381 ctgaaatcca ccatgcccag gcataacgat gcgatatagc cgacacatag cccaatggcg
    52441 atccctccca tgcgtagcaa cggactgcga cagcagttaa agccgatcac cacaattaaa
    52501 accaataaac caacgccgag atgttcgtaa ttgccgaacg taccgctgct tttggctgca
    52561 aatcctccac caaaatcgat aatgccgact ttaatcaggc ttaagccgat catcagtacc
    52621 acaataccgc tgacggtagg cgtaataacc cgacgtaaat agggcaggat aaaagaagag
    52681 ccgacaacca gaaatgcgcc aacgaaggag acgccgagga gcgacgacat gattaactct
    52741 tcgtgaaaac cgtcgctttt catgctgctg cccagcgcaa tcatgaccgt aacaaatgaa
    52801 aaattgactg actgaattga gagaagacca gaaccgacga tgccgtagcg gtttacttgt
    52861 aaccaggtac caataccaga ggcgatcatc gccatagaaa caagataggc agttgtttca
    52921 gcggaaagct gtaaggtcgc acccacgatt aacgcggggg ttaccatcgg aacgaaaatt
    52981 gccaacagat gggtaatggc acctacgaga gcctgatgaa agggagggcg atcctccagt
    53041 tcaaatataa ggtcagaacc tgcatggttt atatcagac
//
LOCUS       DAZUCC010000007        51957 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZUCC010000007 DAZUCC010000000
VERSION     DAZUCC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.007"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..1034
                     /gene="ybhD"
                     /locus_tag="ACHY09_000324"
     CDS             81..1034
                     /gene="ybhD"
                     /locus_tag="ACHY09_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3883342.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1075..2070)
                     /gene="pgl"
                     /locus_tag="ACHY09_000325"
     CDS             complement(1075..2070)
                     /gene="pgl"
                     /locus_tag="ACHY09_000325"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFS3883343.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSEDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2225..3043
                     /gene="ybhA"
                     /locus_tag="ACHY09_000326"
     CDS             2225..3043
                     /gene="ybhA"
                     /locus_tag="ACHY09_000326"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFS3883344.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3044..4102)
                     /gene="modC"
                     /locus_tag="ACHY09_000327"
     CDS             complement(3044..4102)
                     /gene="modC"
                     /locus_tag="ACHY09_000327"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFS3883345.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4105..4794)
                     /gene="modB"
                     /locus_tag="ACHY09_000328"
     CDS             complement(4105..4794)
                     /gene="modB"
                     /locus_tag="ACHY09_000328"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFS3883346.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4794..5567)
                     /gene="modA"
                     /locus_tag="ACHY09_000329"
     CDS             complement(4794..5567)
                     /gene="modA"
                     /locus_tag="ACHY09_000329"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3883347.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5733..5882)
                     /gene="acrZ"
                     /locus_tag="ACHY09_000330"
     CDS             complement(5733..5882)
                     /gene="acrZ"
                     /locus_tag="ACHY09_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFS3883348.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6011..6799
                     /gene="modE"
                     /locus_tag="ACHY09_000331"
     CDS             6011..6799
                     /gene="modE"
                     /locus_tag="ACHY09_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFS3883349.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARNHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6867..8339
                     /gene="modF"
                     /locus_tag="ACHY09_000332"
     CDS             6867..8339
                     /gene="modF"
                     /locus_tag="ACHY09_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFS3883350.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARAQAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            8600..9616
                     /gene="galE"
                     /locus_tag="ACHY09_000333"
     CDS             8600..9616
                     /gene="galE"
                     /locus_tag="ACHY09_000333"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS3883351.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9626..10672
                     /gene="galT"
                     /locus_tag="ACHY09_000334"
     CDS             9626..10672
                     /gene="galT"
                     /locus_tag="ACHY09_000334"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFS3883352.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10676..11824
                     /gene="galK"
                     /locus_tag="ACHY09_000335"
     CDS             10676..11824
                     /gene="galK"
                     /locus_tag="ACHY09_000335"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS3883353.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDATIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11818..12858
                     /gene="galM"
                     /locus_tag="ACHY09_000336"
     CDS             11818..12858
                     /gene="galM"
                     /locus_tag="ACHY09_000336"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFS3883354.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPF
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYTDWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            13061..13813
                     /gene="gpmA"
                     /locus_tag="ACHY09_000337"
     CDS             13061..13813
                     /gene="gpmA"
                     /locus_tag="ACHY09_000337"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS3883355.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13961..15013)
                     /gene="aroG"
                     /locus_tag="ACHY09_000338"
     CDS             complement(13961..15013)
                     /gene="aroG"
                     /locus_tag="ACHY09_000338"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFS3883356.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            15329..15709
                     /gene="ybgS"
                     /locus_tag="ACHY09_000339"
     CDS             15329..15709
                     /gene="ybgS"
                     /locus_tag="ACHY09_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFS3883357.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15823..16764
                     /gene="zitB"
                     /locus_tag="ACHY09_000340"
     CDS             15823..16764
                     /gene="zitB"
                     /locus_tag="ACHY09_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFS3883358.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16761..17480)
                     /gene="pnuC"
                     /locus_tag="ACHY09_000341"
     CDS             complement(16761..17480)
                     /gene="pnuC"
                     /locus_tag="ACHY09_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS3883359.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQMFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17518..18561)
                     /gene="nadA"
                     /locus_tag="ACHY09_000342"
     CDS             complement(17518..18561)
                     /gene="nadA"
                     /locus_tag="ACHY09_000342"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFS3883360.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCSVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPV
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18884..19876)
                     /locus_tag="ACHY09_000343"
     CDS             complement(18884..19876)
                     /locus_tag="ACHY09_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3883361.1"
                     /translation="MSIKQLKDGRYKVDVRPQGTEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIEAWWLLDGRNQDYGDTYRVRLEKINREMGDPRASQMT
                     RKFMLGYRSDKLQAGLMPSSINRDLCALSAMFSSLIEGEVFHNENPVRGIRKLKVRNT
                     EMAFLSDEEIERLLERLDGDARRVAILCLSTGARWGEAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDAVLALIKTRRTGLLYQADYLRFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLAESVHKLSID"
     gene            complement(19946..20293)
                     /locus_tag="ACHY09_000344"
     CDS             complement(19946..20293)
                     /locus_tag="ACHY09_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3883362.1"
                     /translation="MNSTLSISIGEKILMIRKSERLTRKQFFELTGIPVNTQRYYETG
                     RQDGIGSEILLKITKNLRFKKYALWLTTDETFEAGGQIAPALSLDGSSQSEGDLASID
                     VTQKSSRSGRNAG"
     gene            20395..20919
                     /locus_tag="ACHY09_000345"
     CDS             20395..20919
                     /locus_tag="ACHY09_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFS3883363.1"
                     /translation="MRDFIADKELSDTDHTTGAAYINADTSQQEATDNNDEQKPQRTY
                     KKGATLRLDGPIAGLCSLEKGAAYIGLTKTALRAALQRNQMPGHKTRSNPEDENSDGV
                     WWFNVREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFTPADKEDCQIVNGKRVYKG
                     RKSKLEQSQDVNER"
     gene            20926..21321
                     /locus_tag="ACHY09_000346"
     CDS             20926..21321
                     /locus_tag="ACHY09_000346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883364.1"
                     /translation="MSGKNKSEKNATVLKQLSLLKEEHKDATILMHLECSPKITITTT
                     YSELHKELEGLINFYSKLKANENIDNLILKPEAEFGHFCESRENGCIGIMLYATRGIM
                     INLPVEQAVDIFGEMALNKTTIYKRKMPE"
     gene            complement(21335..21529)
                     /locus_tag="ACHY09_000347"
     CDS             complement(21335..21529)
                     /locus_tag="ACHY09_000347"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883365.1"
                     /translation="MSYDDELIKMLKSVIEIVDKIPEVPPSSDDKHDQIRDLANDVRE
                     KVLHKYPRKSSWNFSKGPTY"
     gene            21637..21972
                     /locus_tag="ACHY09_000348"
     CDS             21637..21972
                     /locus_tag="ACHY09_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFS3883366.1"
                     /translation="MTQRRSFRTGTERHANRFSTSASRSNSHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPWGN"
     gene            21976..22230
                     /locus_tag="ACHY09_000349"
     CDS             21976..22230
                     /locus_tag="ACHY09_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883367.1"
                     /translation="MATTTKDMAEIASAYTIITHWLIANNARNALNTAKPLWEAKEDI
                     ISALQGLQKCADLAGDYAAYMAIEDTIEKIESGKSLSDFV"
     gene            22251..22493
                     /locus_tag="ACHY09_000350"
     CDS             22251..22493
                     /locus_tag="ACHY09_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFS3883368.1"
                     /translation="MDKEYKVLINKALERFNFRLSMSGVAAEHAARDSLTRAIRSLYD
                     AAFYADDLDALNELSELVRDAECGVHIEPYALGNAA"
     gene            22490..22615
                     /locus_tag="ACHY09_000351"
     CDS             22490..22615
                     /locus_tag="ACHY09_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883369.1"
                     /translation="MSIFLSWVILIISVLMAIGIMRIIEALKNIESLFTGEERRK"
     gene            22695..22973
                     /locus_tag="ACHY09_000352"
     CDS             22695..22973
                     /locus_tag="ACHY09_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883370.1"
                     /translation="MSTNTPTEINKLFTDPAHIELTQKTLTEITNVLDERISEIDKDK
                     HFATYMALQMQSMAMVTAKQTISELYMKNLRLERELAELRAQSGQFSA"
     gene            22985..23227
                     /locus_tag="ACHY09_000353"
     CDS             22985..23227
                     /locus_tag="ACHY09_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883371.1"
                     /translation="MNSIFTATMLTRFTDAVGHEFMVESHLITTTTPCPSDADYLYIH
                     LADGTQITAIASTVREVMAIRGAWKSETQAHGELRP"
     gene            23224..23436
                     /locus_tag="ACHY09_000354"
     CDS             23224..23436
                     /locus_tag="ACHY09_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883372.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            23463..24014
                     /locus_tag="ACHY09_000355"
     CDS             23463..24014
                     /locus_tag="ACHY09_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3883373.1"
                     /translation="MRLTPMGIPGKAPAHVKPWTQQEDKLLAALYHSHTTPELTKHFQ
                     RTQCAIQSRIRILLSQGVIHAKQKPLNKKELNTLIKNRHTKTIRELANELGRSPSTVE
                     LNLRKRGYRFRKYGELHHRARYSDQLVVLVTELRDEQGMTFSEIRKYIRDTTGISLKR
                     WVPARLYARYTAADTVLCELLPD"
     gene            24335..24562
                     /locus_tag="ACHY09_000356"
     CDS             24335..24562
                     /locus_tag="ACHY09_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFS3883374.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLWRASRVAQIMYINMLHEHAH
                     ALSDRYCAAVFSYLADDLAELQDLINQQHHH"
     gene            24631..25047
                     /locus_tag="ACHY09_000357"
     CDS             24631..25047
                     /locus_tag="ACHY09_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883375.1"
                     /translation="MKNVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMALVLEMTNHSV
                     AVKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            25044..27563
                     /locus_tag="ACHY09_000358"
     CDS             25044..27563
                     /locus_tag="ACHY09_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFS3883376.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQVIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLHHDEERRIRLEKLARHKHENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSTALERACDEVIEKTS
                     RGDDDLLTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTAPDPEQVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMTCARGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRFFAAVRRTIDKAGLGWYGVRTVEPHHDGTPHW
                     HMLVFTSQENEARITEIMRNAAIREDRAELGDDISPRFKCEKIDPAKGTPTSYIATYI
                     GKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAGLHRIHQFQFFGIPPRQV
                     WRELRRLAGQMARNPAAPQRLDHDDIDAILAAADVGCFATYITRQGGVLIPRNTYLVR
                     TAYETAEEANDYGEFPQRIYGVRAPSLGERYTICTHPDTWKLVRRKPENEDRTDEGFD
                     VECGFAAPWTRGNNCPPDQNLTINDPPERQPDGRREQRLTLPEGDLGEWLRSLTPTER
                     KQLTRQLKNPPPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALGVQLHE
                     SAVVSVRHGARVRLADGRTAYWNDSTRRIDITPAQNTEAPQ"
     gene            27560..27964
                     /locus_tag="ACHY09_000359"
     CDS             27560..27964
                     /locus_tag="ACHY09_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883377.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTTIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLVTINRKAAIARYVHSAAGRQSRR"
     gene            28013..28183
                     /locus_tag="ACHY09_000360"
     CDS             28013..28183
                     /locus_tag="ACHY09_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883378.1"
                     /translation="MNDTRRISDRELTKMIESARQLLSRQNPVVEETWWENLLAALTE
                     LRERRNNEGAAK"
     gene            28180..28506
                     /locus_tag="ACHY09_000361"
     CDS             28180..28506
                     /locus_tag="ACHY09_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS3883379.1"
                     /translation="MKTPGMTGKEIILRYLETHTRFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAAFR"
     gene            complement(28572..28835)
                     /locus_tag="ACHY09_000362"
     CDS             complement(28572..28835)
                     /locus_tag="ACHY09_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFS3883380.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(28873..29925)
                     /locus_tag="ACHY09_000363"
     CDS             complement(28873..29925)
                     /locus_tag="ACHY09_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS3883381.1"
                     /translation="MSKKRRNKTSRVTKKAFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAHMGFLLYTNDPGITTEMED
                     EIREKVAESKGLGNFRNLYINIPKGSPDGVKIIPIGEVSAKDEFSNIKGISAQDVFTA
                     HRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKIMNAVNSDPDIPEHLYLCF
                     DVEAGHVAEEKAVPVG"
     gene            complement(29922..31727)
                     /locus_tag="ACHY09_000364"
     CDS             complement(29922..31727)
                     /locus_tag="ACHY09_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFS3883382.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAVELGIPPRTVYHWCDVGR
                     WAALLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAE
                     IAALRPAGHEDGVAAGICGGGEGKKRRYRKNDVSGLTAEMLDEWARKNLYAYQLHCRE
                     NRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIIDFAQ
                     QFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMA
                     THDKYRTTYFSTPSAKTHQAYLVWNGDDWRGDDPTRRAVEFPKESAMRVGCECPDGIW
                     RYIIRLEEAVAGGLSARVDIERIRNRYNPTTYAMLYGCEFVDSKDAVFKFSELVRCEV
                     EMETWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAPPVHEGERFRVLAVWQWQ
                     GFNFTWQADQIRELMQRFNITYIGIDITGIGKGVFDIVSRFAPREANAILYSVESKNR
                     LVMKMIDVVAHKRIEWAKDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDV
                     TGHADVFFAISHAVINEPVDYDYERPSTWAFGKAS"
     gene            31894..32943
                     /locus_tag="ACHY09_000365"
     CDS             31894..32943
                     /locus_tag="ACHY09_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFS3883383.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     LLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTSPPPRKPESFLKRLFSAGQTDNTDTSKPDTGEDAKMDEIKALLEKLITLVEGGS
                     AAAAGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAEEVKAAPEDEVKAEAFTAAKA
                     KLEDAIKAFSTITPEKGERYRRFAARRRDDTDKEEIKAIAEKLNDLTTKLSATGITPR
                     PAGTPAESEKPFDFV"
     gene            32955..33983
                     /locus_tag="ACHY09_000366"
     CDS             32955..33983
                     /locus_tag="ACHY09_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFS3883384.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGGENEFFNRIQNFVNRQIALDILRIAFNGTSVAEDTDPVTN
                     KNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEAA
                     EEPASGGA"
     gene            34001..34699
                     /gene="gpM"
                     /locus_tag="ACHY09_000367"
     CDS             34001..34699
                     /gene="gpM"
                     /locus_tag="ACHY09_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFS3883385.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIADGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTTTWR
                     LHEEIRAKWFRFAGLYLLRDEAGKPRATAINDTAVLEKADAYLAQAAALSRTAGVKSI
                     RARIRARISALNAA"
     gene            34807..35280
                     /locus_tag="ACHY09_000368"
     CDS             34807..35280
                     /locus_tag="ACHY09_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFS3883386.1"
                     /translation="MSEPSFSVSGKPLKSTPQTVTNGEPFWPDLDLAELQGSRTLPAD
                     LPDDTAVMALLAAVAEVNTTLAPVVAHWKAQGHERAADVPGARLGDETQLVAQYKKAV
                     YARAKADLMGEFATIGRRETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            35277..35750
                     /locus_tag="ACHY09_000369"
     CDS             35277..35750
                     /locus_tag="ACHY09_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3883387.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMELFVSRSRE"
     gene            35747..36457
                     /locus_tag="ACHY09_000370"
     CDS             35747..36457
                     /locus_tag="ACHY09_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883388.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQTAPDGTAWAPRKRSKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTSGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQNGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITVMEDQIIAGPRQQKARTWTITLPSRVFLGVTNEEFSHILA
                     RQMNAMGYGLDVDAQDIR"
     gene            36469..37614
                     /locus_tag="ACHY09_000371"
     CDS             36469..37614
                     /locus_tag="ACHY09_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFS3883389.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDESLLKSTLTAAIANGGQNWFAYVHVLSEPKPPAPEGGDANAAWVDAVK
                     KAQTIASAEGVVIAIDITAKDAVNRATETRALLQSAYGRFVWFMLCVAGPGRDEAWAA
                     YVTRISAIQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPARVATGALVAMG
                     NDEIPQDGKKQPLELATLRSLEGKRFSVPMWYHDFDGLYWSDGRTLDVEGGDYQVIEN
                     VRVVDKVSRRVRLRAIARLADRALNSTPGSIAASQTYFSRTLREMALATEINGVPFPG
                     EVKPPQDGDITIVWTSSTHVQIYIVVRPYESAKAIGVSIELDTALEI"
     gene            37616..38077
                     /locus_tag="ACHY09_000372"
     CDS             37616..38077
                     /locus_tag="ACHY09_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFS3883390.1"
                     /translation="MAGNERISGGAFDINYDGIMIHVESATVTITDNSAVAQTRGVPN
                     GYTRGSVSADVEVEVDAQNFKKFGAVARSAGSWRAIPEKDFLFYANNGSEEEKIEVFG
                     CVPMLSDVVNISAGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            38082..38387
                     /locus_tag="ACHY09_000373"
     CDS             38082..38387
                     /locus_tag="ACHY09_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFS3883391.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFKFPDMPELVVVGLACALGIMGSAFIEECFCRLADRYLKQKERRNDP"
     gene            38377..39129
                     /locus_tag="ACHY09_000374"
     CDS             38377..39129
                     /locus_tag="ACHY09_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS3883392.1"
                     /translation="MTHKPYISPSLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYINDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRASRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCITHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRND"
     gene            39129..39467
                     /locus_tag="ACHY09_000375"
     CDS             39129..39467
                     /locus_tag="ACHY09_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFS3883393.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KSGKGIHNSLHTLRLAVDFNLFINGEYQADTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGMK"
     gene            39464..39850
                     /locus_tag="ACHY09_000376"
     CDS             39464..39850
                     /locus_tag="ACHY09_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883394.1"
                     /translation="MMKQLAGCLVILFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVRPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDGR"
     gene            39950..40225
                     /locus_tag="ACHY09_000377"
     CDS             39950..40225
                     /locus_tag="ACHY09_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFS3883395.1"
                     /translation="MTTKNTGTIILSVAGVTLSFEPNKTAFNNLINEITMTNKVAPMS
                     TYLNRIVTPASRDALADLIARYPGCEMQIVEKINEVYSPKLEIELKN"
     gene            complement(40276..40416)
                     /locus_tag="ACHY09_000378"
     CDS             complement(40276..40416)
                     /locus_tag="ACHY09_000378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883396.1"
                     /translation="MSPSVKCDRYTFSGLVGAGTGKIFIQPDGPGDALFVVRLSGEVV
                     TP"
     gene            40415..42601
                     /locus_tag="ACHY09_000379"
     CDS             40415..42601
                     /locus_tag="ACHY09_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS3883397.1"
                     /translation="MASQLDFIINLTDQLTRPLRQVQESVTGFAERSQGAFARIGVGV
                     AGLWGVAEGIRAAMDPAWEMQKALGELEAKGVGAQALTALTTQAMKLGTAFGQSSTEI
                     IRSAWHIRGALDGLSDGDLPRVTAATSTLAVAARVSSEEASGYLAAMGARFRSEFSAM
                     GAVGFAEDLAGKTAWMVQQFGVEMSAMQELIEGTKNAGADFGVSMAEQFAILGTLSRT
                     MGTEASGIYEEFLRSAPAAAQKMGMSFVDATGKILPMGDILDRLQARFGRNIEGNVAA
                     QQALDDAFGSGADVIKKLWGQQEMLNRSISALGRNDGMKRATEMAEAMTDPWDRLVAS
                     FYNIRAAIGNALYPILAPLAGRFADIGATFVRWLNMFPNIARWLGYITLTMLALGAAG
                     AVTNIVLGVFSFIMEGHKAIIKGARAAWSLYTLALTKVRSAIIATSLAARLAGVSFLT
                     MSAPVLLVAAAIAALVVVAIKFWQPIKAFIRGFIDGFTSALDAMSPVTAAFGDIARAL
                     APVWQGIKTLFSAFSDLFAPVQLTTGQMNSVTRAGETCGRVVAAAVSMISPALEVVIS
                     VIRTLIDIFAIVIRGWQDVVAAFDPTAPVESFRKMAGIIGNVFGDLWNVIKNSFNRTW
                     NWLAEKLNKIPGISIDMKPTEQVNTMTRGATGSTVPPQIAETAVPALAGNRISAELPP
                     GGIRNQIRTDTSTRIDNSRTFGNLTINTQNPMTPGQLDEWTELYAG"
     gene            42598..42948
                     /locus_tag="ACHY09_000380"
     CDS             42598..42948
                     /locus_tag="ACHY09_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFS3883398.1"
                     /translation="MNTPSLYIDLLITDGSFTLNSGNEPQRCDNRVSIGQDVVHSIIE
                     SGIAALLIGERSPTLRSDIITRLILLVEDDERLVPGTVFINEEAPGRLHVTAETQDFG
                     RINTRVNYERETGR"
     gene            42926..44110
                     /locus_tag="ACHY09_000381"
     CDS             42926..44110
                     /locus_tag="ACHY09_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFS3883399.1"
                     /translation="MKGKPDVDFNQVFRESGIPETEEEIATLFHEDVQKTGLITNTSP
                     MSPFWRLILTIVIRPVQWLRDALVDVVLPGMFVATADRSLLLLHAHDVNITPKPASKA
                     SGVIWFAKTRANDDVLIPEGTIVQTARINGVIYSMTVTEETLISSGKSGASVPVEATT
                     EGSGHNLAPGYFRILPVAIPGIASVVNREDWLTVPGADEESDDELRDRIRNQFNLVSS
                     YHTDAVYRGLIASVAGLSIDRIYFQHDAPRGPGTANAYLLLDSGEASAPFIEAVNRYI
                     NEEGHHGHGDDLQCFAMPETRHRLEVTVYIRSSTTLPPDGAEGLKQDIGDLIRCAFRE
                     NARFNVKKVWPYSRFSFSTLGKEIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVV
                     IE"
     gene            44103..44651
                     /locus_tag="ACHY09_000382"
     CDS             44103..44651
                     /locus_tag="ACHY09_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3883400.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAAQAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTEIYRRRISYAFINAQEAGEVAGFIAIFE
                     RLGIGYVELLERQPGVDWDVILVQVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     NPFIIHYGAYEGEYICWTARAE"
     gene            44674..46740
                     /locus_tag="ACHY09_000383"
     CDS             44674..46740
                     /locus_tag="ACHY09_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3883401.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVGKTGVVNENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAM
                     IVHAPTQKKIRTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQTWQIDFTARLASMDE
                     RQRRENVDIYGKATFFDDGWLVKKGETWTVNKGVGYVEGLRAELSTHHPMTTPAFPSR
                     IWLNVWWTGTLTSEWATEHEIITADPDVTEERLRKTYPRDGIQQYFCAIAEIDEDGNV
                     TDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLSSATTSTSETQAATPKAI
                     KIAMDNADGRLAKNRNGEDIPNKELFLINVGAPRVYAKDGYVGKAGQRWTTEQFVEWL
                     NERGAFRPGLWIFKANISLSQHSVLTDTGYGDICLGGCLIEVSGDINGGLIRITTGIE
                     SPEDITRPHRTQFTGVILSKGKSAIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPW
                     PSDTPPDGYAIMVGQTFDKAQYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQD
                     GIKSHTHGGSVSNTDLGTKTTSSFDYGTKTTSTNGQHEHSVPLRRQDGGGINFDWLDG
                     SSSGSHIGNGYVPAAGDHQHTVPIGAHIHTLALGQHGHGLTINPTGNAENTVKNIAFN
                     YIVRLA"
     gene            46740..47336
                     /locus_tag="ACHY09_000384"
     CDS             46740..47336
                     /locus_tag="ACHY09_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS3883402.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENAWHLMEDHRGKTVWRLESGSALLITEPGPLPENVTMIKP
                     DGEFCRWDGSSWVKDEAAEQAYRVSEAENKKRKLIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLLSRTDTGNAPDIVWPESPSPDHHAG"
     gene            47348..48073
                     /locus_tag="ACHY09_000385"
     CDS             47348..48073
                     /locus_tag="ACHY09_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883403.1"
                     /translation="MTWTRKTISVSADMPPLSCSVIPVTPWTYGLGRYEESGVYLSPP
                     NAINWLAGKMAGSRASGDVTIIMIAENTHDLFIQSMAALTAVLPVPVFTQAQRMAQAA
                     ASLSTDKMQIPITTDTLPAPVHLSVATMRSAVSAARAAQARQLAAQRPDAAGLRRQIS
                     RFISRRAGALAGLEDGIRTLAEQKAQAWVFQYRGYHRAAAAAMVKDIPAPTAVHTVAA
                     LLAADSLTELGKMIHEPDRTARP"
     gene            48045..48596
                     /locus_tag="ACHY09_000386"
     CDS             48045..48596
                     /locus_tag="ACHY09_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883404.1"
                     /translation="MSQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVTGMITFDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFS
                     GQIDAQQQTDRLAWQVSFTLRERRSVPEKRQARAAPGSSRKQTPGTRQTPGAKGKTVA
                     DETPEKMTWFEEKVLKPVNDALE"
     gene            48599..50272
                     /locus_tag="ACHY09_000387"
     CDS             48599..50272
                     /locus_tag="ACHY09_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883405.1"
                     /translation="MNPTKRLLLGNDTIQLVSCSVMLELNACGRGFITARTEQNYTGR
                     PVRLDIGYGNDLVRWFTGYVERSQPAENGSVRLLIREMAGILDHPFPCSFQHPTMRTV
                     TQWLSETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPDYIWHPLPDG
                     GIMAAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNGQRLTMVMLE
                     DDNMTITWTPRNKATGAPLQKSPFRRQAEKAFPELASGLHLPKLARVEAPTEAVSAGN
                     IADPFRPRYAVNLQLLDENGNPAADTPVYNAVPLPVPMAGSESGMFQFPPPGTLVEVG
                     FVEGRADKPFIRQIMAEGHNLPDVKPGEQLQQQRDGVSQRVTVAGDWERQTDQRIQET
                     SRERVVSADEETRSLTSRETTIQATDTTTVLGVSTLQAGAVTHITEENYSISTGGHMM
                     VVAGTCERDVEGDTLDTVGGSLTEKIKGVRHSIAQAQKLVGDTVKLGNEGINVLTMLT
                     DLADVVEELADITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE"
     gene            50675..50980
                     /locus_tag="ACHY09_000388"
     CDS             50675..50980
                     /locus_tag="ACHY09_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883406.1"
                     /translation="MVLHFVQGFAKVCKNSGFLRIPKNFNALNNKRFLKLRHDRRSEK
                     GSDDENEKIRKARTGAAWQGVAAKITFAHFCTTAPKKDGEGEGKTATSSVARNGRKR"
     gene            51137..51277
                     /locus_tag="ACHY09_000389"
     CDS             51137..51277
                     /locus_tag="ACHY09_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3883407.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHKSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            51608..51748
                     /locus_tag="ACHY09_000390"
     CDS             51608..51748
                     /locus_tag="ACHY09_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3883408.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
BASE COUNT        12975 a        14447 c        13750 g        10785 t
ORIGIN      
        1 taatttaagt tggctaaatg cagttaaaat ttttaactgc cagtcaccca aaatcgtcgc
       61 ttccattttc cggagtaata atgaagcatg aattatcaag tatgaaggca tttgtcatac
      121 tggcagagtc cagttcattt aacaatgctg ctaaattact caatattacg caacctgcat
      181 taacgcgaag aataaaaaag atggaggaag atttacacat tcaactattt gagcgtacaa
      241 ctcgcaaggt tacgttaaca aaagcaggaa aaaggttgct cccggaggcg cgggaattaa
      301 taaaaaaatt cgatgaaacg ctttttaata ttcgtgatat gaatgcttat catcgtggta
      361 tggtgacgtt agcgtgtatt ccgaccgcag tattttattt tttaccgctg gcaatcggta
      421 aatttaatga gctgtatccc aatattaaag tgcggattct ggaacaaggc acgaataatt
      481 gcatggaatc ggtgctgtgt aacgaatcag actttggcat aaacatgaac aacgtcacaa
      541 attcatccat tgattttacc ccactggtca acgagccgtt cgtgctggcc tgtcggcgtg
      601 accatccatt ggccaaaaag cagcttgtag aatggcagga gttagtgggt tacaaaatga
      661 ttggcgtacg ctcctccagc ggcaaccgac tattgataga acaacagcta gccgacaagc
      721 cctggaagct ggactggttt tacgaagtgc gtcatctttc gacgtcatta gggctggtgg
      781 aggcgggact ggggatttcg gcgcttcctg gtctggcaat gccccatgcg ccctattctt
      841 cgattattgg tatcccgctg gtggaaccgg ttatacgtcg gacattgggg attattcgcc
      901 gcaaagatgc cgtgctttct ccggcagctg aacgcttttt tgccttgctg attaatttgt
      961 ggactgacga taaagacaat ttgtggacca atatcgtcga acgccagaga catgcgttac
     1021 aggagatagg ttaatacacc ggcgcagaag agactgcgcc gggtaaatca gcggttagtg
     1081 tgcgttaacc accacccaca ttggtccctg cccgaccgca tagcggcctt tttcatgcag
     1141 tagcccctgc tcgccaacaa tttcgtatac cgagatgtgg tgagattttt gcccggcggc
     1201 aatcagatac ttgccgctgt gatcaacatt gaagccgcgc ggctgggttt cagttggctg
     1261 gaagccttct ttactcaaca cgctgccatc ttccgaaacg ctgaaaacag taatcagact
     1321 ggcggtacgg tcgcaggcgt ataaatgacg accatccggg gtgatatgaa tatcagccgc
     1381 ccaacgggtg tcggagaagt tttccggcat catatccagc gtctggacac attcgatatt
     1441 gccatgcgga tctttcagtt cccagacatc cactgagctg tttaactcat tgacgcaata
     1501 cgcatattgt tcgtttggat ggaataccat atgacgcggg ccggcccctt caacggtagt
     1561 cacttccgca gggtcctgcg ccaccagatg accatcctcg ctgacagtaa acaggcaaat
     1621 gcgatcctgc tttaatgccg gaacccacag cgtacggttg tccggtgaga tattggcgga
     1681 atggcaaccg tccagcccct cgaccacatc gacgacgccc actggcaggc catcttccag
     1741 acgcgttacg ctcacgttac ccgcattgta agaaccgaca aagacaaact gcccctggtg
     1801 atcggtggaa atatgcgtcg gactacccgg cagcgcagac tctgcggcaa aggtcagcgc
     1861 gccatcgtcc ggggcgatac gatacgccag gacgcgaaac tcagggcgaa caccaacata
     1921 gagataacgt ttgtccgggc tgaccaccat cggctgcacc tgccccggca cgtcgacaac
     1981 ctgtgtcagc gtcagtgcgc cttcatgatt caggttccag acgtgaattt gctggctctc
     2041 agggctggcg atataaactg tttgcttcat gaatgctcct ttgcatttag caactgactg
     2101 taacagctaa aattagtcgc ttttggcggt gaatgctgaa ttaaaacgca gaattttgtc
     2161 cgatagtggt atcggtgtac cattcgcgca gacgaaaatt cttaacctta atctggaata
     2221 cgccatgacc acacgcgtga ttgctctcga cttagacggc accttattga ccccgaaaaa
     2281 gaccttgctt ccatcatcga tagaagccct ggcccgcgct cgcgaagcag gctatcaatt
     2341 aatcatcgtc acaggtcgcc atcacgtcgc tattcatcct ttttatcagg cgctggcgct
     2401 ggatacacct gctatttgct gtaatggcac ctatttgtat gattatcatg caaaaacagt
     2461 gctggaagcg gacccaatgc ccgttaataa agccctgcaa ctcattgaga tgctgaatga
     2521 acaccacatt cacggtctga tgtatgtcga tgatgcaatg gtctatgagc acccgaccgg
     2581 acatgtcatt cgcacgtcta actgggcgca aaccctgccg ccggaacagc gtccgacttt
     2641 cacacaagtc gcttctctgg ctgaaacggc gcaacaagtt aacgccgtat ggaagttcgc
     2701 cctgacgcac gatgacctgc cgcaattgca gcattttggt aagcatgtcg aacatgaact
     2761 gggactggag tgtgaatggt cctggcacga tcaggttgat attgcacgcg gcggcaacag
     2821 caaaggtaaa cgtttgacga aatgggttga ggcgcaaggc tggtcgatgg aaaacgtcgt
     2881 ggccttcggc gataacttca atgatattag tatgctggaa gccgctggta caggcgtggc
     2941 gatgggcaac gccgatgacg cagtaaaagc gcgcgccaac attgtgattg gtgataacac
     3001 caccgacagc attgcccagt tcatttatag ccacctgatt taatcaggcg gttatcgaca
     3061 cacttttaat ttgcgcatac agccacaggc caggtttgat cgccagttca tccctggccc
     3121 acgggctgat acgcgcccac agcgttttac cgccgacttc cagctccact tccacctggc
     3181 cgttgtcgtc ataactatta accacttttg cccgcaatac gttacgaatg ctggtttgct
     3241 gcggcggttg taaaaccagc gagacatcgg aagcctgaat gcggatgcgt agcgcagctt
     3301 gcagcggttc gtccagctta ttgacccaca aatgctgatc gcccagcgcc agcgcggtca
     3361 tcgcgtaatg cggatgatgc tcaagcaccg tcactttcag aatgctactt tgttgctctt
     3421 tcggcagcca cggattcatc acgctactgc cccacacttc ctccagcgcg ccaaaggctt
     3481 tcacctgacc gttttccagt accatcactc tgtctgccag atggaggatc tcatccagcg
     3541 aatggctgac atacaacatc ggaatgttga tttcccgtgt cagccgttgc agataaggca
     3601 acagttcgcg tttacgcgga atatccagtg acgccagcgg ttcatccagc aacagcaatt
     3661 ccggtgctgt cagcaaagcc cgaccaatcg ccacgcgctg tttttcacct ccggacaggc
     3721 tgcctggtaa acggtcaagc aacggttcaa tgcctaaaag cgccaccagc ttatcgaact
     3781 gatcgaccat acttttcgcc atgccgtagc gcagattgcc acgcactttg taatgcggga
     3841 acagccgcgc atcctgaaaa acatagccaa cgcgacgctt ttccggcgtc aggcagatac
     3901 ctttttcggc atcatttagt acccgcccat tgaggacaat ccgccctttt tgcgggcgcg
     3961 tcagtccact gatggcgtta atcagcgaag tttttccggc accggagacg ccaaagatag
     4021 cagtgatgcc attggcgggc agcgtttcat taatggtcag gcaatggttg cccaacgtct
     4081 gggaaaaatt cagttccagc atgattagcg ccccgcccgt tcacggctga ttctggccag
     4141 ccattctgaa atcaacaggg agatcatcgc cagtgcaata gaaataatgc acagtctcgc
     4201 cgctccactt tccccgccgg gggtctggat cagggtatac atggcagaag gaatggttcg
     4261 cgtttcacca ggaatgttcg acacaaaggt gatggttgca ccaaactcac cgagagaacg
     4321 agcaaaagcc agtaccgtac caacaataat gcccggtaag gtcagcggta acgtgatagt
     4381 aaagaaaacg cgccagcgcc cggcacccag tgttcttgcg gcctgttcca gtttgacatc
     4441 aaccccttcc agcgccagac gaattgcccg caccatcagc ggaaacgaca tgacggcggc
     4501 agcgagaacc gcgccgcgcc agctaaaggc gaaggtaatg ccaaaccagt catacagacg
     4561 ttcaccgata aatccgcgcc gtcccatcga aactaataat aagtaaccga cgaccacggg
     4621 cggtaacacc agcggtagat gcagtacgct gtcgagcaga gctttgcccg gaaacgtgca
     4681 acgcaccagt aaccaggcaa aaaagatccc aaacggcagg ctaaacagca cagccaggga
     4741 agaaactttc aggcttagta aaaccgcctg ccattctgga tcggtcagta tcattacttg
     4801 gttgtaaatc cgtaacgttt aaagatttcc gcagcctgcg gtcccttcag ataatcataa
     4861 aatgctttca ccgtcgcatt gttatgccct tccacaacag caaccggata ttccactttt
     4921 ttatgcgaat cttccgggaa ggtggcaacc acttttaccc ctttgctggc aactgcgtca
     4981 gaaccgtaaa caatacccag cggcgcttca ttgcgttcga ccaacgccag cgcgccacga
     5041 acatcttccg ccggagccag tttcggggag agcgtatccc atgcgcccag tttttgcagt
     5101 gcttcttttg cataaatgcc agcgggaaca tgttccggat cgccaaccgc cagacgaccg
     5161 ccattcagca gcgaagtcca gttggttttg ctgtcgatgg tgaaatcttt ctgctcgctg
     5221 gctttcggtg ctacaacgac caggctattg ccgagcagtg tctggcgcga agccgtatcg
     5281 atcgcttttt tatcaaccgc ataatccatc catttctgat cggcagaaat aaacagatcc
     5341 gcaggcgcac ccgcttcaat ctgacgggcc aaagtagatg acgaagcgaa agaagaaacc
     5401 acatccacgc ctttctcttt tttatactgc gtagcaatgt cctgcattgc gttagtcagt
     5461 gatgcggcgg cgaacaccgt gattttccct tcatctgcca gtgcattgcc agcaacagcg
     5521 aaagagagtg ccgccccggc aaacaagttc aaccatttac gagccattgg taacccctta
     5581 atctaacgtt atgtaggcga caatataacg gctaactcag aattttccca gcagttattg
     5641 ctaacctttg tgaggtagat gagaaaaaat atcggcagga aaagcaggag gttgagaaaa
     5701 agaaatgccc gactaagcgg gcattcaggg attcaatgat tttgtccggg ctggtctttt
     5761 ttaccaacac cagaaaagat gttgaatact tcaccaagac cgtaaatcag acccaggatg
     5821 atggccatca cgacaggtac catgattacg gcgaatacca gactttttaa taactctaac
     5881 atggtcaact ccagatatag tcatgagact attctaaccg ctaagcaaag aaaagcactc
     5941 cccttttgtg cggtcagctt tgcgtggcgt tcgttttccg tcacaataag actttttcca
     6001 ggatattgtt atgcaggccg aaatccttct cacccttaag ctccaacaaa aattattcgc
     6061 cgacccgcgc cgcatttcgc tactaaaaca cattgcgctt tccggttcca ttagccaggg
     6121 agcgaaagat gccggtatca gctataaaag cgcatgggat gccattaacg agatgaatca
     6181 gttaagtgag catattctgg ttgagcgcgc aacaggcggt aaaggtggag gcggcgcagt
     6241 actgacccgc tatggtcagc gactgattca gctctatgac ttactggcgc aaatccagca
     6301 aaaagccttc gatgtgttaa gtgacgatga cgccctgccg ctgaacagcc tgctggccgc
     6361 gatctcacgt ttttcactgc aaaccagcgc ccgtaaccaa tggtttggca ccatcaccgc
     6421 ccgcaatcat gatgacgttc aacagcatgt tgatgtctta ctggctgacg gaaaaacacg
     6481 cctgaaagtc gcaattaccg cacaaagcgg cgcgcgtctg gggctggatg aaggcaaaga
     6541 agtgttgata ttactaaaag cgccgtgggt aggcattact caggacgaag cggtcgcaca
     6601 aaacgccgac aaccaattac cgggtattat tagccatatt gagcgcggcg cagagcagtg
     6661 cgaagtatta atggcgctac ccgacgggca aacactgtgc gccacagtgc cggtaaatga
     6721 agcgacttct cttcagcaag gacagaatgt cacggcctac tttaatgccg acagcgtgat
     6781 tatcgccacg ctgtgctaag cgtgttgaca atttgttatg aaacacgtat ccctgtcagt
     6841 aatcgctgca caaaatggga tataaaatgt catcgttgca aattttgcaa ggcacgtttc
     6901 gtcttagcga cacaaaaacg ctgcaattgc ctcagctaac gttaaacgcg ggtgatagtt
     6961 gggcgtttgt cggttcgaat ggaagcggga aatcggcgct ggcccgcgcg caggcggggg
     7021 aacttccgct tttgaaaggt gaacggcaaa gccagttttc ccacatcact cgtctctcct
     7081 tcgagcaatt gcaaaagctc gtcagcgacg aatggcagcg gaataacacc gatatgctcg
     7141 gccctggcga agatgacacc gggcgtacca cggctgaaat cattcaggat gaagtaaagg
     7201 atgcaccgcg ttgcatgcaa ctggcgcagc agttcggtat taccgccctc ctcgaccgac
     7261 gctttaaata cctttccact ggcgagacgc gaaaaaccct gctgtgtcag gcgctgatgt
     7321 cggagcctga cttgttgatt cttgatgagc cgttcgatgg cctggatgtt gcctcacgtc
     7381 agcagctggc tgagctactc gcctcgttac atcagtccgg tattactctg gtactggtgc
     7441 tcaatcgctt cgatgagatc ccggaatttg tccagtttgc tggcgtgctg gcggattgta
     7501 cgttagcgga aacgggcgct aaagaggaac tgctccagca agcactcgtc gcgcaactgg
     7561 cacatagcga acagcttgaa ggtgtgcaac tgccggagcc ggatgaacct tcagcacgtc
     7621 acgccttacc cgccaacgaa ccgcgcattg tgctgaacaa cggcgtggtt tcttataacg
     7681 atcgccccat tcttaataac cttagctggc aggtgaatcc aggcgagcac tggcaaattg
     7741 tcgggccaaa tggcgcggga aaatcgacgt tattaagcct gattactggc gatcatccgc
     7801 aaggttacag caacgatttg acgcttttcg gacgacgtcg cggcagcggc gaaaccatct
     7861 gggatatcaa aaagcatatc ggttacgtca gcagtagttt gcatctggat taccgggtca
     7921 gcactaccgt gcgtaatgtg atcctttctg gctattttga ttcgattggc atttatcagg
     7981 ccgtttcgga tcgccagcaa aaactggtgc agcagtggct ggatattctc ggcattgata
     8041 aacgcacggc tgacgctccg ttccatagtc tttcctgggg acagcagcgt ctggcgctga
     8101 tcgtccgcgc gctggtgaaa catccgacgt tgcttattct cgatgaacca ctacaggggc
     8161 ttgatccgct caatcgccag cttatccgcc ggtttgttga tgtgctgatt agcgaaggtg
     8221 aaacgcaatt gttgtttgtt tcgcaccatg ctgaagatgc gcctgcctgt attacccatc
     8281 gtcttgagtt cgtgccggac ggtgactttt atcgctatgc gctgacaaaa ataaactgag
     8341 tcggtaatgc tgactgtacc ggaggcggcc taagcgccct ctccggccta cagttcgacg
     8401 catccaggca tgaaaccgcg acttttttca gataaaaagc tcaatcattc ataaaccctc
     8461 tgttttataa tccattaatg gcgcataaaa aacggctaaa ttcttgtgta aacgattcca
     8521 ctaatttatt ccatgtcaca cttttcgcat ctttgttatg ctatggttat ttcataccat
     8581 aagcctaatg gagcgaatta tgagagttct ggttaccggt ggtagcggtt acattggaag
     8641 tcatacctgt gtgcaattac tgcaaaacgg tcatgatgtc atcattcttg ataacctctg
     8701 taacagtaag cgcagcgtac tgcctgttat cgagcgttta ggcggcaaac atccaacgtt
     8761 tgttgaaggc gatatccgta acgaagcgtt gatgaccgag atcctgcacg atcacgctat
     8821 cgacactgtg atccacttcg ccgggctgaa agccgttggc gaatcggtac aaaaaccgct
     8881 tgaatattac gacaacaatg tcaacggtac cctgcgcctg attagcgcca tgcgcgccgc
     8941 gaacgtcaaa aactttattt ttagctcctc cgccaccgtc tatggcgatc agcccaaaat
     9001 tccatacgtt gaaagcttcc cgaccggcac accgcaaagc ccttacggca aaagcaaact
     9061 gatggtggaa cagatcctca ccgatctgca aaaagcccag ccggactgga gcattgccct
     9121 gctgcgctac ttcaacccgg ttggcgcgca tccgtcgggc gatatgggcg aagatccgca
     9181 aggcattccg aataacctga tgccatacat cgcccaggtt gctgtaggcc gtcgcgactc
     9241 gctggcgatt tttggtaacg attatccgac cgaagacggt actggcgtac gcgattacat
     9301 ccacgtaatg gatctggcgg acggtcacgt cgtggcgatg gaaaaactgg cgaacaagcc
     9361 aggcgtacac atctataacc tcggcgctgg cgtaggcagc agcgtgctgg acgtggttaa
     9421 tgccttcagc aaagcctgcg gcaaaccggt taattatcat tttgcaccgc gtcgcgaggg
     9481 cgaccttccg gcctactggg cggacgccag caaagccgac cgtgaactga actggcgcgt
     9541 aacgcgcaca ctcgatgaaa tggcgcagga cacctggcac tggcagtcac gccatccaca
     9601 gggatatccc gattaaggaa cgaccatgac gcaatttaat cccgttgatc atccacatcg
     9661 ccgctacaac ccgctcaccg ggcaatggat tctggtttca ccgcaccgcg ctaagcgccc
     9721 ctggcagggg gcgcaggaaa cgccagccaa acaggtgtta cctgctcatg atccagattg
     9781 cttcctctgc gcaggtaatg tgcgggtgac aggcgataaa aaccccgatt acaccgggac
     9841 ttacgttttc actaatgact ttgcggcttt gatgtctgac acaccagatg cgccggaaag
     9901 tcacgatccg ctgatgcgtt gccagagcgc gcgtggcacc agtcgggtga tctgcttttc
     9961 accggatcac agcaaaacgt tgccagaact cagcgttgcg gcattgacgg aaatcgtcaa
    10021 aacctggcag gagcaaaccg cagaactggg gaaaacgtac ccatgggtgc aggtctttga
    10081 aaacaaaggc gcggcgatgg gctgctctaa cccgcatccg cacggacaga tttgggcaaa
    10141 tagcttcctg cctaacgaag ctgagcgcga agaccgtctg caaaaagaat atttcgccga
    10201 acagaaatcg ccaatgctgg tggattatgt tcagcgcgag ctggcagacg gtagccgtac
    10261 cgttgtcgaa accgaacact ggttagccgt tgtaccttac tgggctgcct ggccgttcga
    10321 aacgctactg ctgcccaaag cccacgtttt acggatcacc gatttgaccg acgcccagcg
    10381 cagcgatttg gcactggcgt tgaaaaagct gaccagtcgt tatgacaacc tcttccagtg
    10441 ctccttcccc tactctatgg gctggcacgg cgcgccattt aatggcgaag agaatcaaca
    10501 ctggcagctg cacgcgcact tttatccgcc tctgttgcgc tctgccaccg tacgtaaatt
    10561 tatggttggt tatgaaatgc tggcagaaac ccagcgagac ctgaccgcag aacaggccgc
    10621 agagcgtttg cgcgcagtca gcgatatcca ttttcgcgaa tccggagtgt aagaaatgag
    10681 tctgaaagaa aaaacacaat ctctgtttgc caacgcattt ggctaccctg ccactcacac
    10741 cattcaggcg cctggccgcg tgaatttgat tggtgaacac accgactaca acgacggttt
    10801 cgttctgccc tgcgcgattg attatcaaac cgtgatcagt tgtgcaccac gcgatgaccg
    10861 taaagttcgc gtgatggcag ccgattatga aaatcagctc gacgagtttt ccctcgatgc
    10921 gaccattgtc gcgcatgaaa actatcaatg ggctaactac gttcgtggcg tggtgaaaca
    10981 tctgcaactg cgtaacaaca gcttcggcgg cgtggacatg gtgatcagcg gcaatgtgcc
    11041 gcagggtgcc gggttaagtt cttccgcttc actggaagtc gcggtcggaa ccgtattgca
    11101 gcagctttat catttgccgc tggacggcgc acaaatcgcg cttaacggtc aggaagcaga
    11161 aaaccagttt gttggctgta actgcgggat catggatcag ctaatttccg cactcggcaa
    11221 gaaagatcat gccttgctga ttgactgccg ctcactgggg actaaagcag tttccatgcc
    11281 gaaaggtgtg gctgtcgtca tcattaacag taacttcaaa cgtaccctgg ttggcagcga
    11341 atacaacacc cgtcgtgaac agtgcgaaac tggtgcgcgt ttcttccagc agccagcgct
    11401 gcgcgatgtc accattgaag agttcaacgc tgttgcgcat gaactggacc caatcgtggc
    11461 gaaacgcgtg cgtcatatcc tgactgaaaa cgcccgcacc gttgaagctg ccagcgcact
    11521 ggagcaaggc gacctgaaac gtatgggcga gttgatggcg gagtctcatg cctctatgcg
    11581 cgatgatttc gaaatcaccg tgccgcaaat tgacactctg gtagaaatcg tcaaagctgt
    11641 gattggcgac aaaggtggcg tacgcatgac cggcggcgga tttggcggct gtatcgtcgc
    11701 gctgatcccg gaagagctgg tgcctgccgt acagcaagct gtcgctgaac aatatgaagc
    11761 aaaaacaggt attaaagaga ctttttacgt ttgtaaacca tcacaaggag caggacagtg
    11821 ctgaacgaaa ctcccgcact ggcacccgat ggtcagccgt accgactgtt aactttgcgt
    11881 aacaacgcag ggatggtagt cacgctgatg gactggggtg cgactttact ttccgcccgt
    11941 attccgtttt ccgatggcag cgtccgcgag gcgctgctcg gctgtgccag cccggaatgc
    12001 tatcaggatc aggccgcatt tctgggggcc tctattggtc gttatgccaa ccgtatcgcc
    12061 aatagccgtt atacctttga cggtgaaacc gtgacgcttt caccaagtca gggcgttaac
    12121 cagctgcacg gcggcccgga agggttcgac aaacgtcgct ggcagattgt gaaccagaac
    12181 gatcgtcagg tgctgtttgc cctgagttca gatgatggcg atcagggctt cccgggtaat
    12241 ctcggagcga cggtacaata tcgtctgacc gacgataacc gtatctctat tacttatcgc
    12301 gccacagttg ataaaccttg cccggtgaat atgactaatc acgtctattt caatcttgac
    12361 ggcgagcagt ctgacgtgcg caatcacaag ttgcagattc tggcggacga atatctgccg
    12421 gttgatgaag gcggcattcc ccacgacggc ctgaaatctg tcgccggaac gtcttttgat
    12481 ttccgcagcg ccaaaatcat cgccagtgag tttcttgccg acgacgatca gcgcaaagtg
    12541 aaaggttacg atcacgcgtt cttgttacag gccaaaggcg atggcaagaa agtggcggcg
    12601 catgtctggt cagcagatga aaaattgcag ctgaaggtct acaccaccgc tccggctctg
    12661 caattctact ccggcaacta cctcggcggc acaccgtcgc ggggaaccga accttacacc
    12721 gactggcaag ggctggcgtt ggaaagcgag tttctgccgg acagcccgaa ccaccctgaa
    12781 tggccgcaac cggactgttt cctgcgtcct ggcgaagagt attccagcct gacggaatat
    12841 cagtttattg ctcagtaatg ttaagccctc gtcatgaggg ctttatcttc atattgttca
    12901 aatcaccagc aaacaccgac atatttgcaa ctcaatattc acaacaacct tacactgcga
    12961 cactattttc gctatggtta tgcgtaagca ttgctgttgc ttcgtcacgg caatataatg
    13021 agaattatta tcattaaaag atgatttgag gagtaagtat atggctgtaa ctaagctggt
    13081 tctggttcgt catggcgaaa gtcagtggaa caaagaaaac cgtttcaccg gttggtacga
    13141 cgtggatctg tctgagaaag gcgtaagcga agcaaaagca gcaggtaagc tgctgaaaga
    13201 ggaaggttac agctttgact ttgcttacac ctctgtgctg aaacgcgcta tccataccct
    13261 gtggaatgtg ctggacgaac tggatcaggc atggctgccc gttgagaaat cctggaaact
    13321 gaacgaacgt cactacggtg cgttgcaggg tctgaacaaa gcagaaaccg ctgaaaagta
    13381 tggcgacgag caggtgaaac agtggcgtcg tggttttgca gtgactccgc cggaactgac
    13441 taaagatgat gagcgttatc cgggtcacga tccacgttac gcgaagctga gcgagaaaga
    13501 actgccactg acggaaagcc tggcgctgac cattgaccgc gtgatccctt actggaatga
    13561 aaccattctg ccgcgtatga agagcggtga gcgcgtaatc atcgctgcac acggtaactc
    13621 gttacgtgcg ctggtgaaat atcttgataa catgagcgaa gaagagattc ttgagctgaa
    13681 tatcccgact ggtgtgccac tggtgtatga gttcgacgag aatttcaaac cgttgaaacg
    13741 ctattatctg ggtaatgctg acgagatcgc ggcgaaagca gcggccgttg cgaaccaggg
    13801 taaagcgaag taaacgtcat tcgcttaaaa atgagaaagc cgactgcaag tgagtcggct
    13861 tttttgtttg tcgttgctaa caatggagca cattgcctga tgctacgctg cgcgtcttat
    13921 cagggctgca aacgtacagt catggcgatt cgactataaa ttacccgcga cgcgctttta
    13981 ctgcattcgc cagttgacgt aacagagcat cggtatcatc ccagccaatg caggcatcgg
    14041 tgatgctctt accataggcc agcggttccc cactctcgag gctctgattg ccttccacca
    14101 gatggctttc caccatcacg ccaataatag ccttttcgcc accggcaatc tgctggcaaa
    14161 cgtcagcaca aacatccatc tgctttttga attgttttga cgagttagca tggctgaaat
    14221 cgatcatcac ctgtgctggc aggcctgctt tgttcagccc ttctttcact tcagcaacgt
    14281 gcttcgcgct gtagttaggc tctttaccgc cgcgcagaat gatatggcaa tcgccgttac
    14341 cgctggtatt cacaatcgcc gaatgccccc atttcgttac ggacaggaag cagtgcggcg
    14401 caccggcggc attaatggca tcgatagcca ctttaatcgt accgtcggtg ccatttttga
    14461 agccgaccgg acaagaaagc cctgatgcca gttcgcggtg cacctgcgat tcggtggtac
    14521 gtgcgccaat tgcgccccag ctcatcaggt cagcgagata ttgtggggtg atcatatcca
    14581 ggaattcacc cgccgctggc agaccgctgt cgttaatatc aagcagcaat ttacgggcaa
    14641 tacgcagacc gtcgttgatc tggaagctgt tatccatatg cggatcgtta atcagccctt
    14701 tccagcccac cgtggtacgc ggcttttcaa aatagacgcg catcacgatt tccagctcat
    14761 cttgcagctc ttcacgcagc gtcagcaagc gagtggcata ctctttcgcc gcgacaggat
    14821 catgaattga gcacggacca atcaccacca acaggcgatc atcattacct ttcaggatct
    14881 tatggatcgc ttttcgggca tgggcgaccg tattcgcggc attttcagta gcggggaatt
    14941 tttccagcaa tgcgactgga ggaagtaact ctttgatttc tttgatgcgt aaatcgtcgt
    15001 tctgataatt catgtctgtt ccagtgttgc catacttatc ttagtgaatg caatacttcc
    15061 aatctatatc ttccgtcaga atgtgtaaac ggggttttac actatgaacg gattaatcct
    15121 ggaattagca agaaaatcgc cagattgtcg cgaaaaacga gatctctcct acaatttcta
    15181 actgtaactc ctttgtttgt taattatttc aagattctct gctgggtatc tttgcctggc
    15241 tgaaatctta aaccattgcc ttatattcac ctgcaaatgc actgttggaa gaggttatcc
    15301 gacataacga ccataacagg agcatcctat gaaaatgaca aaactggcca cactattttt
    15361 gactgccact ctaagccttg ccagcggtgc cgcactggct gccgatagcg gagcgcaaag
    15421 caataacggc caggcaaacg ccgcagctga tgcgggccag gtagcccctg atgcccgtga
    15481 aaatgtcgcg ccaaataacg tcgacaataa cggggtaaat accggttctg gcggcacaat
    15541 gctgcatccg gatggttctt caatgaacaa tgacggcatg accaaagatg aagagcacaa
    15601 gaacaccatg tgcaaagatg gtcgctgccc ggatatcaat aaaaaagtcc aaaccggtga
    15661 tggcatcaac aatgatgtcg ataccaaaac cgacggtacc acgcagtaac cctgcgaaat
    15721 agccaataat gggagagcgg agtctctccc ttttaatttt tcccttctaa aagcagtatg
    15781 atgtcataaa gttaagtgag aataattaag ggatgactcg tcatggcgca ctcacactca
    15841 cacacttcct cacacctgcc agaagataat aatgctcgtc gcttgttgta tgctttcggc
    15901 gtgactgccg ggtttatgct cgtagaagtc attggtggtt ttctttccgg ttctctggca
    15961 ttgctggccg atgcgggtca tatgttgacc gatactgccg ccctgctttt cgccctactc
    16021 gcagtgcaat tttcccgtcg tcctcccacc attcgccaca ctttcggttg gctaagattg
    16081 accacgctcg cggcgtttgt gaatgccatc gccctggtgg tgattactat tttgattgtc
    16141 tgggaggcga tagaacgctt ccgcactccg cgtccggtcg agggcggcat gatgatggca
    16201 attgccgtgg ctgggctgct ggcaaatata ctttctttct ggttacttca tcacggcagc
    16261 gaagagaaaa acctcaacgt gcgagcagcg gcactgcatg tgctcgggga cctgctgggt
    16321 tcggttgggg ctattattgc cgcgttgatt attatctgga ccggctggac acctgctgac
    16381 cccattctct caatactggt gtcgcttctg gttctgcgta gcgcgtggcg actgttgaaa
    16441 gatagcgtga atgaattact tgaaggtgca ccggtatcgc tggatatcgc cgaactgaag
    16501 cgtcgtatgt gccgggaaat cccggaagtc cgcaatgtgc accatgtgca tgtatggatg
    16561 gtaggcgaga agccggtgat gacgctgcat gtgcaggtga tcccgccaca cgatcacgat
    16621 gccttgttag atcagatcca acactacctg atggatcact atcagattga gcacgccact
    16681 attcagatgg aatatcaacc ttgtcatggg ccagactgcc acctgaatga aggggtgtct
    16741 ggccattcac atcatcacca ttaatgggac agcgcgcgtg aacctctttc gcgtgcgctg
    16801 ttgatccaca tccggctgcc gttgagggca ataaaggtca ggatgatgta ctccagagac
    16861 atggcgtaaa cgccctgaag tgcaaaaata acgacgctaa tcacgttaat aatcacccac
    16921 aacagccagt tttccacata cttacgcgtc atcagaatca ttgccacgat agataacacc
    16981 atcatgcatg aatcccagaa cgggaaagcg tccggctgta gttcaggcat cgcaacctgt
    17041 aatcctaatg cttgcatgat catgactgcc acgcgggtca aaaatgcaaa caccggattg
    17101 ataaataccg tcatcagacc aatcgaaaca acgcaaaccg ccaaccagct tagcgccttc
    17161 ggcaatggca accagcgaat tttcaactcc gcctcgttct gactggtttg tcgcgaccac
    17221 gcataccagc cgtaaatatt cgcggcaaag aaaaacatct gtaatagcag gctggcatac
    17281 agctgaatct gaaagaaaat aatgccaaac aaggtgacgt tgatcaggcc aaagaagtag
    17341 ttgctgatct tctccagact ggcaaggcca atacacagca acccggcgat cgtgcctacc
    17401 gcttcgatcc atgagagatc ataaccgcct gcacctattg gtatatgtac caggatattc
    17461 tgcacactaa aaaaatccat ctttccccca aagcgtaacg ttacgcctta ttattcgtta
    17521 tccacgtagt gtagccgcaa aatccagcat acgattgagc ggcaccagcg ccctctctcg
    17581 cagcctttca tcaacatgaa cctcgtgatt gcttccttcc tgttctaatg cctctgcgat
    17641 ggcctgaagg ccattcatgg ccatccacgg acaatgcgcg cagctgcggc aggttgcacc
    17701 ctcacctgcg gttggtgctt ccagtaactc tttaactggc accgcctgct gcattttgta
    17761 gaaaataccc cgatcggttg ccacaataag cctctgatgt ggcaatgttt tcgcagcagc
    17821 gatcagttga ctggtggaac cgaccgcatc cgccatctct acaatagctt gcggtgattc
    17881 tggatgcacc agtatggcag catccgggta ttcttcttgc aagcgggtta acgcctgagt
    17941 cttaaactca tcatgcacaa tgcaggcacc ctgccagcat aatatgtctg cgcccgtctg
    18001 tttttgcacg taacgcccca gatgtttgtc gggtgcccag atgatttttt cacccaaact
    18061 atccagatga tcaataagtt cgacggcaat gcttgaagtt accacccaat ctgcgcgcgc
    18121 ttttaccgca gcagaagtgt tggcgtagac gacgacagta cgatcgggat gggcatcgca
    18181 aaatgcgtta aattcttcaa cagagcagcc gagatccagt gaacattcag cctgaagtgt
    18241 cggcatcaga attgtttttt ccggactgag aattttggcg gtttctccca taaatctcac
    18301 cccagcgact aacaacgtag aagcgggatg ctttgcaccg aagcgcgcca tttccagaga
    18361 atcagaaata cagccaccgg tttcttctgc cagttgttga atttcgggat cggtatagta
    18421 gtgggcaacc atcaccgcat tacgttcttt tagcagacgt tttatcttct cgcggtaata
    18481 cgctttttca tcaatgctta acggcgtcgg cttcggggga aaaggataaa tcgccgtgtc
    18541 tggatcaaac atcacgctca tcttaccatc tcgttttaca ggcttaacgt taaaaccgac
    18601 atgagcgtaa tattcgctgt tatcggtgaa atttgtttta tatgctaaac aagatagtcg
    18661 aatggtgggg aaaagtcact cgaattacgc ctaattccac cgacgtagag ttgcttgcaa
    18721 agtaagctct gaagatgtca tcaggttaca aagtgaaatt gaggatgcct gaatagcaaa
    18781 aaagcgcctt taggaggttt aacgctactt actccccttg atgatttgct tatttttcaa
    18841 tgagtaaatc aaaacaaggg gagcaaataa gcacaagtta caactaatcg atggacagtt
    18901 tatggacact ttcagccagc ggattaaagc tgattgcatc ctgtaaaaag tccggcgaaa
    18961 aatgcgcata cgtcatcgtt tgttgaatcg tggcatggcc caggattcgt tgtagcgtaa
    19021 cgatatttcc gccattcatc atgaaatgtg tagcgaatgt atgacgcatg acatgggtgg
    19081 cctgcccttt cggtaagtcc ggcttcacct cctgaaggat ctcccgaaat ctgaggtaat
    19141 cagcctggta caacaatccg gttctgcggg ttttaattaa cgcaagtacc gcatcagaaa
    19201 cgggaacggc gcgagatttt ccgttcttgg tgttaaagaa catcacgcgg ttacctacaa
    19261 tatgttcccc acgtaaacca gctgcttcac cccaccttgc ccccgtggaa agacacagaa
    19321 tggcaacacg ccgcgcatct ccatcgagtc gttcaagcag tcgttcaatc tcctcatctg
    19381 aaagaaaagc catctctgtg ttcctgactt tcagcttacg tattccccga accgggtttt
    19441 cgttatggaa cacctcaccc tcaatcagcg agctgaacat tgcagacaag gcacacaaat
    19501 cgcggttaat gctggaaggc attaacccgg cctgtaattt gtcggaccgg tacccgagca
    19561 taaattttcg cgtcatctga ctggcacgag gatcacccat ctcacggttt atcttttcca
    19621 gtctgaccct gtaggtatcc ccgtaatcct ggttacgacc atcaagcaac caccaggcct
    19681 ctatcagttc ggataaccga cgctgatctg ctggcttagc ctgccatggc ttatcatgaa
    19741 aattctgcaa aacatacctt tcaaactcct gcgcctttga cttcagggta aaaatcttac
    19801 gaatccgctt tccttccgtc ccttgtggcc ttacatcaac cttataacgt ccgtctttga
    19861 gttgcttaat cgacatgatt agcccctcca acggataaat taaccaaaca ctccctgcgg
    19921 gatgaaaccc gctcgatgtg taacgtcagc cagcattgcg gcctgatcgg gatgattttt
    19981 gggtaacgtc gattgaagcc agatcgccct ccgactgaga agatccatca agagagagag
    20041 ccggggcaat ttgaccccca gcttcaaacg tttcatcagt tgttaaccaa agtgcatatt
    20101 ttttaaaacg taaatttttt gtgatcttta ataagatctc acttccgatt ccatcttgcc
    20161 ttcccgtttc gtaatacctc tgcgtattaa caggaatccc tgtaagctca aagaattgct
    20221 tccttgtaag cctctcactt ttccggatca tcaagatttt ttcaccgata cttattgaca
    20281 gtgtcgaatt cataccacta aaatcctcgc aaaagtgtcg taatttcgac attaaacaaa
    20341 ccaaaacaaa ccttcataag gtgcaatagg cgcacctgtg cgagaggata acaaatgcgg
    20401 gacttcattg ccgataaaga gttatctgac accgatcaca caactggagc ggcgtacatc
    20461 aacgccgata ccagtcaaca agaagcaaca gataacaacg acgaacaaaa gccacagcgc
    20521 acatataaaa aaggggcaac cctgcggctg gatggcccaa tagccgggct ttgctcactt
    20581 gaaaaagggg ccgcctacat cggattaaca aaaacagcat tacgcgcagc tcttcagcgt
    20641 aaccagatgc cagggcataa aacccgctca aaccctgaag atgagaattc ggatggtgtt
    20701 tggtggttta acgtcagaga gtgggacaag ctggccgacg aactcccgga atgtgagcca
    20761 ccagaatggc acaactggaa aagctactgg acatacgacc gccagaaaag aaagtttacc
    20821 ccggctgata aggaagactg ccagatcgtc aatggtaagc gggtttacaa aggcagaaag
    20881 tcaaagctgg aacagagcca ggatgtaaac gaacggtgat gaacgatgag cggcaaaaat
    20941 aaatcagaaa aaaacgcaac cgtccttaag cagttaagcc tcctgaaaga ggaacacaag
    21001 gacgccacga tactgatgca tctggaatgc tcaccgaaaa taacaatcac aacaacctat
    21061 agcgagttac acaaagagct tgaaggatta attaactttt attcaaagct aaaagcgaat
    21121 gaaaacattg ataacctgat acttaaacca gaagcagagt tcgggcactt ttgcgaaagt
    21181 agagaaaatg gatgcatcgg aataatgcta tacgcaaccc gcggaataat gattaatctg
    21241 cccgttgagc aggctgtgga tatattcgga gaaatggcac ttaataaaac cactatttac
    21301 aaaagaaaaa tgccggagta acccggcatg gttattagta tgttggtccc ttagaaaagt
    21361 tccaggaact cttgcgaggg tatttatgaa gtactttttc gcggacatcg ttagccagat
    21421 ctctgatttg atcgtgctta tcatcagaag acggaggaac ttcggggatt ttatccacga
    21481 tttcaattac tgatttaagc atttttatta attcatcgtc gtaagacatt gttatctcct
    21541 ttttctggtt gtgtggaaac accagaatac cacgcgccgg gcgtggttaa acatcccggc
    21601 actgattttc atttaactta aacggggttt tccgatatga cacagcgacg cagcttccgc
    21661 accggaacag aacgccacgc taaccgtttc tcaaccagcg catcacgcag caactcgcac
    21721 tacagcctgc aggaaacgca cgccacacct gacgggcatc tggttaaaca aaccggtaaa
    21781 catacctggc tgattaatga tgccggaatt gtcattcacc ggggcgcacg caacccgatt
    21841 accgggaata cctgttactc cctgactcgc ggagatgagc agttcgggca ggatttcacc
    21901 ctgcacgaag cattaagaac ggcagaccgg ttaatcagcg gtcgcagttt tcagcatcca
    21961 tgggggaatt aatacatggc aacaacgaca aaagacatgg cagagatagc atcagcctac
    22021 acaattatca cacactggct catagctaat aatgcccgta atgcactaaa tacagcaaaa
    22081 cctttatggg aagcaaaaga agatatcatc agcgcattac aggggctaca gaaatgcgcc
    22141 gacctcgcag gcgattacgc cgcatacatg gctattgaag acacgataga gaagattgaa
    22201 tcaggtaaat cattaagcga ttttgtttaa ttgttttccg ggaaaataca atggacaaag
    22261 aatataaggt tctcatcaat aaagcccttg agcgctttaa ctttcgcctg agtatgtcag
    22321 gagttgccgc agaacatgca gcaagagact cactgaccag agcaattcgc agcctgtatg
    22381 atgcagcttt ttacgctgac gatctggatg ctctcaatga gctgtctgag cttgttcgtg
    22441 atgcagaatg cggagtacat attgagccat acgcacttgg gaatgcagca tgagcatatt
    22501 tctttcatgg gtgattctga ttatttccgt gcttatggcg attggcatta tgcgaattat
    22561 cgaagcgctg aaaaatatcg aatctttatt tactggcgaa gaacgccgta aataaataac
    22621 caaccggaca gaaaacatta aaaccatccg catctgcgga gggcttcact caatccagaa
    22681 acggagaaac agctatgagc accaatacac ctacagaaat aaacaaacta tttaccgacc
    22741 cggcgcatat tgaattgacg caaaaaacgc tgactgaaat aaccaatgta ctggatgaaa
    22801 gaatatcaga aatagataaa gacaagcatt ttgctactta catggccctg caaatgcaaa
    22861 gcatggcaat ggtgacagca aaacagacca tcagtgaact ctatatgaaa aacctgcgcc
    22921 tcgagcgcga actggcagaa ctccgggcac agtccggcca gttttcagcg tgaggggcag
    22981 gtcgatgaac agcatattta ccgccaccat gctgacgcgt ttcaccgatg ccgtgggcca
    23041 cgagttcatg gtggaaagtc atttgatcac aacgaccacg ccgtgcccgt cagacgctga
    23101 ttatctctac atccatctgg ctgacggcac acaaatcacc gcgattgcca gtaccgttcg
    23161 cgaagtaatg gccatcagag gggcatggaa atcagaaaca caagcccacg gagagctgcg
    23221 gccatgatca gcaagtacac caccaacgca cgtaaacccg gtaaagacgc acgccaccac
    23281 atcgccgtac tccgcaatct gttactggag gaaatcgacg acttcacggc aacgctgagc
    23341 ggtcctggcg caccagtaac accaggtgag atccaccggg accttaaacg ccgtattgaa
    23401 aacgccattg attacaccct gaatccgacc gaataaccgg aacagtaacg gaggttatcg
    23461 cgatgagact tacaccaatg ggaataccgg gcaaagcccc ggcacatgta aagccgtgga
    23521 cacaacagga ggacaaactg ctggccgcgc tataccactc ccacacgaca ccggaactga
    23581 caaagcactt tcagagaact caatgtgcaa tacaaagccg catccgtatt ttgctctctc
    23641 agggggttat tcacgccaaa cagaaacccc tgaataaaaa agagctcaac acgctcatca
    23701 aaaatcgcca cacaaaaacc atccgggaac tcgcgaatga actggggcgc tcgccatcca
    23761 cggtagaact gaatctgaga aaaagagggt atcggttcag gaaatacggg gaactccacc
    23821 acagagccag atacagcgat caactggtgg tgctggtaac tgaactgcgc gatgaacagg
    23881 ggatgacatt cagcgaaatc agaaagtaca tccgggacac gaccgggata tcccttaaaa
    23941 gatgggtccc cgcccgactt tacgcacgat ataccgccgc cgacaccgtg ctctgtgaac
    24001 tgttaccgga ctgagtgatc atcatgcaca cacaaaaaaa ccgcttgcca gcgttcgcct
    24061 ggtcgggtta cagttttccc gcacctcata aaacgggtgc cgggtttcgc agcctgctga
    24121 ctacacaagc gcacaaccgc gccagcggtt tttttgtgcg tactgtatcg ccacgtcttt
    24181 ttcgcacacg aattatggcg gggcgtacgg ggccgacttc ggtcgggccg ggttcttgtg
    24241 tagccggtac tgcgaacctc gtacgcctcg ccacccacag tttcgcagct ctggatggtg
    24301 agttttcaca acttactaca caaggggcca caccatggca aaccgcaagc aacagcgcgc
    24361 ccgcgctgag cgtcttcaca tccgcaccga aatcaaccgc agactctggc gcgcatcacg
    24421 cgtcgcgcaa atcatgtaca tcaacatgct gcatgaacac gcacacgcgc tgtcagaccg
    24481 ttactgtgcc gccgtcttca gctacctggc ggatgacctg gcagaactgc aggaccttat
    24541 caaccagcaa caccaccact aaacaacatc ctgaagccat cccgcacccg tgcgcgggag
    24601 gcttccgcac gtctgttacc ggaggttctc atgaagaacg ttaagggact ttcagatatt
    24661 caggattttt ttaactggat gtgggcagtg gatccccggc tggcttcccg cctgaacgac
    24721 tggcacgact attaccgccg cgcctgtaaa caggccgcca gacgcgtcac cacatacggg
    24781 gatttctcct tcaccgttga tgaccgttac cgcgtggacg tgaaaggcga tggtgatggt
    24841 tttggtttat ttcacatcaa cggcaccgac agcaccgcca tcagctggtc gtcgtcagag
    24901 atggcgcttg tgctggagat gacaaaccac agcgtggccg tgaaaggcat atacaccgca
    24961 gaagacttta tcggtgaata ccagcgcctt ctcgtgctgt accgcgcggc gtttcaggcg
    25021 gcgactgccg gggagtgcga cgcatgagtg accacaatgc acagctggcg tacccgtgga
    25081 acgccccgct accggttatc gatcctgaaa cgttcggcag agcgcagcca accgcattac
    25141 gccaggtcat tcagagctac atcaacgagg atattcgcac tgacgccaga ctggatgaag
    25201 aaaccgtcgc cttcctgaca accacagacg aagggaagcg aattaattcg catcttcatc
    25261 acgacgaaga gcgtcggatc agactggaaa aactggcgcg gcacaaacac gagaatccgc
    25321 ccacactggt atctgaagcc atggcggagc tgcgcgccct gccgccgttt cttgccggtc
    25381 cgctcatccg ggacctggca cgcctgagcc gcaaacagga gagcgcacgc cgggaagggc
    25441 ttaagaacaa cagcgcatac gcggcggata acttcgttcg tcacgggctg cgtgcccgcc
    25501 tgaaacgtat cgcccgtgtg aatgaacgct ttgccacccc ggcctttaag gccaccgccg
    25561 cccgcgaacg gctggatgaa ctgcttatgc tgccgcagct gagccgtgac gaaatccagc
    25621 gccttgccac cctgaccgcc accgcgtttt ccaccgcact ggagcgcgca tgtgatgaag
    25681 tcatcgaaaa gaccagcagg ggtgacgatg acctgctcac ctggttactg acctaccagc
    25741 cactggcaca catggcgtta aaactgggta tcacgccacc ctgctggccg tcgctggaaa
    25801 tcaggcccga ccgccgcacc gcccccgacc cggaacaggt gccgggtgcg gtcatgcgcc
    25861 tttcctgtgc gacatggtgg aacagtcagt taagacacct ccgggacatc tggcgtgaag
    25921 agctgttacg cgccgcaggt ctggtgtcac gtaagacatc ggtatatatc agccatgaag
    25981 cgttactgga ctggcgcgaa aaacgccagc gcacccgcga ttttctgaag gcctgggaca
    26041 tcgagaacga agagggagaa cggttcagcc ttgaggatat ctactgggca ggtctctcta
    26101 atccgcgtaa ccgccgtaac gagatgatga cctgcgcccg gggaatggag caggtcgccg
    26161 aagcacgcgg cgacgtggcg tttttcgtca ccgtcacagc cccttcccgt tttcacagcg
    26221 tgaacgatga cggcagcctg aacccgaaat actgcggagc aacggtacgc gatgccagtg
    26281 attatctggt ttaccgcttt ttcgccgccg tccgcaggac catcgacaag gccgggctgg
    26341 gctggtacgg cgtgcgcacc gtggagcccc accacgacgg tacaccgcac tggcacatgc
    26401 tggtattcac cagccaggaa aacgaagccc gtatcactga aatcatgcgc aacgccgcca
    26461 tcagggagga ccgcgcagag ctgggtgatg atatctcccc gcgttttaaa tgcgaaaaaa
    26521 tcgacccggc aaaaggcaca ccgacaagct atatcgcgac ctatatcggt aagaacctgg
    26581 acgccagcgc cttcatgggg aatgacccga aaaccggcaa accttacgtc gataaggaat
    26641 caggcaaaac catggccgaa accgtggaga acgccatcgg ctgggcaggc cttcaccgca
    26701 tccaccagtt ccagttcttc ggtatccctc cccgccaggt atggcgcgaa ctccgccgcc
    26761 ttgccgggca aatggcccgt aacccggctg ccccgcagcg tctggaccat gacgacattg
    26821 acgccatact ggccgccgcc gacgtgggat gttttgccac ctatatcacc cgccagggtg
    26881 gtgtgctcat tccccgcaac acgtacctgg tccgcaccgc atacgaaact gccgaagagg
    26941 ccaacgacta cggcgaattc ccgcaacgca tttacggcgt ccgcgccccg tcactcgggg
    27001 agcgctacac catatgcacc cacccggaca cctggaaact ggtcagacgt aagccggaaa
    27061 acgaagaccg cacggacgag ggttttgacg ttgagtgcgg ctttgccgca ccttggactc
    27121 gtggcaataa ctgtccccct gaccagaatc tgaccataaa cgacccgcca gaaaggcagc
    27181 cggacggacg cagggagcag cgacttaccc tgccggaagg tgatttgggt gaatggttaa
    27241 ggtcactgac ccccacagag cgaaaacaac tcaccaggca gctgaaaaac ccgccacctg
    27301 acacggaaac cgtgaaaaac ggcggcatca gtgcgcccgc tggcggcgtt cgcgtaagtg
    27361 tcgcactgac cgctgagcca cacatcaccg cacaaatcac ccgtgagctt gatgcgctgg
    27421 gcgtgcagct gcatgaaagc gccgtcgttt ccgtccgtca cggtgcccgc gtgcgcctgg
    27481 ctgacggacg caccgcgtac tggaatgaca gcacccgccg cattgatatc acccccgcac
    27541 agaatacgga ggcgccacaa tgacccgctt ttattgcgcc atgcagcgcc agcgttttga
    27601 cgaaatgatg cgccagcgca cgcccttact gaccctgaac aacattgcca cggggccaca
    27661 accgggcgat gagctggaaa tcacggtcgt cagtcccgat cgcaccacca cgaccatcat
    27721 caccgacata ctcaacggac gaccggacag caccaaccgc agctggcttg tcgccgttcg
    27781 tccgcgccgt ccgcccggag agccggagcc gctcacgcct gccggaaaaa tcatgcggca
    27841 gtaccacgaa gagcaggcac acaaacacaa cgcagaactc accagaacac tggtcacaat
    27901 taaccgcaaa gcggcaattg cccgctatgt gcattcagcc gctggcagac aatcacgcag
    27961 gtaaacaaag accatgaaca taaccagaaa cagccaacta aaggagcgca ccatgaacga
    28021 caccaggagg atcagcgaca gagagcttac gaaaatgatt gagtcggccc ggcaactgct
    28081 ttcacgacaa aacccggtcg tggaagaaac gtggtgggag aatttacttg cagcactgac
    28141 cgaactccgg gaacgccgga acaacgaagg agccgccaaa tgaagacccc aggaatgacc
    28201 ggaaaggaaa tcattttacg ctacctggaa acgcacacac gtttcagcgc cccggaactg
    28261 gtagaaaaac tgggcgtgaa aagaaccgtc attaatcagg ccgccgggaa aatggtacgg
    28321 gaaggcctgc tggttgtgga cgggtacacg gacaagataa cgcattaccg gaagccaacg
    28381 gaaaaggaac gacaggaact ggcgcaacgg gctgaacggc agcaggaaaa atcagtcatc
    28441 gaagcctgca agcgcagcga aatcatgaag cgaattctgt ttatctacgg cgctgcattc
    28501 cgttaaagga gctggtgagt cggggtaaaa attctttttg ccccggcgaa gcagcagaca
    28561 agcgaagcgc gtcaggaacg ccgcgacagt agttcaagcg cataccggcg atcttccggg
    28621 cgcatggcat caacaagaaa cttcaccata ttgcgaccgg agcgggcacc ggggctgagg
    28681 gtatgggaaa aggtcatcgt acagacgaag gtgtgcccgc attcgacatc actgcatgca
    28741 cagtacagat ccgcaagttc aggggttttc cagtctgtct tccggatgac agcaggtgcg
    28801 ccacagtccg gacaccgggc tttaaaaatc cgcatcttac ctcccgataa cacgtattcc
    28861 ggggaggaca ttttacccga ccggcaccgc tttttcttcc gccacatgcc cggcttccac
    28921 atcaaaacaa agataaaggt gttccggtat atcgggatcg ctgttcacgg cgttcatgat
    28981 tttacgctga agcggtatca cctcatcctt gcggtacgtt tcacgcgtcg tttccgggtt
    29041 agggatggtg gccccgttcg tggggataat ccccgcaaga ccggcaggaa agcggtgtgc
    29101 cgtaaacaca tcctgggcgc tgatcccctt gatgtttgaa aattcatcct tggcgctcac
    29161 ctcaccaatc ggaataattt tcaccccatc cggcgaacct ttggggatgt tgatgtacag
    29221 attgcggaaa tttcccagcc ccttagactc cgccaccttc tcgcggatct catcctccat
    29281 ctccgtggtg atccccgggt cgttcgtata caacaaaaac cccatatgcg ccccgttgtt
    29341 gtagtaacgg cgacgaaaaa ggctggcctc actgttcagc agcgccgagt gaatgccacc
    29401 gatataatcc ggcagcccgt aaatttgctg acgcggatca tacatccgca gaaagataat
    29461 atcttccggc gtatagacca gcgcggggcc ttcctgaagc accacgaact caccggtttt
    29521 gcggcgccgg agatacagcg acggaagcgg ctcaagtgcc acgacgtcgc cgaatacgtt
    29581 acgcaccttg agcagggcga gatcgccgaa caacaggtag tcaaataccg cctgttccat
    29641 ctggtccgtt gtgaggccac caccggtata gcctcccgcc accatgttac ggcgggcata
    29701 cagcacccca ccgtgctggg cgttaaggtt cgggagctgc gccagtgcca ggcggttaat
    29761 cggcagctgc cagtggttgt gttcgttgtc ataccagatg ttatggtagt ccgtgccggt
    29821 ggtaagcacc ggttccggct gaccaaaggt gattgtgctg ccacgtgcgg gcgtgaatgc
    29881 ttttttcgtc acgcgtgacg ttttgtttct gcgtttcttg ctcatgaggc tttcccgaag
    29941 gcccatgtgg acgggcgttc atagtcataa tctacaggct cattaatcac ggcgtgcgaa
    30001 atagcaaaaa acacatccgc atgccccgta acatccgacc gttccgccac gaacgtcagc
    30061 gcgttaccgc tttttgtggt ggtgcggcgt atggacatga acgacgcggg gatctccgtg
    30121 cgttcacggc tggcctcatc aatggcgtct ttcgcccact cgatacgctt atgggccacc
    30181 acgtctatca ttttcatcac caggcggttt ttgctctcca cgctgtaaag aatggcgtta
    30241 gcctcacgtg gcgcaaagcg actgacgata tcaaacaccc ctttcccgat accggtgata
    30301 tctatgccga tataggtaat gttaaagcgc tgcatcagtt cgcggatctg atcggcctgc
    30361 caggtgaagt taaatccctg ccactgccag acggcgagca cacggaaccg ctcgccttca
    30421 tggacaggcg gagccacaat cacaaacgtg gaattatccc cggaccttga cgggtcaaag
    30481 cccgcccaca cctcacgatt gccgaacgga cgtgccgccg tgggatcgta gtcaccccac
    30541 gtctccatct ccacctcaca cctgaccagt tcggagaact taaataccgc atccttgctg
    30601 tcaacaaact cgcagccgta cagcatcgca taggtggtcg ggttgtagcg gttcctgata
    30661 cgttcaatat caacccgtgc agacaggcca cccgccaccg cctcttccag ccggataatg
    30721 tagcgccaga tcccgtcagg gcattcacag cccacacgca tcgcgctttc tttcggaaac
    30781 tcaacagcac gacgcgtcgg gtcatccccg cgccagtcgt caccgttcca gaccagataa
    30841 gcctggtggg ttttggcgct gggtgtggaa aaatacgtcg tgcggtattt gtcgtgggtc
    30901 gccattgccg aggccacttc atgcagtcgc gtgaattttg ggatccagaa cacctcatcg
    30961 ccgtaaaggt ggccgttaaa tccctgggcg gtactggcat tggtggacag gaagcgcagc
    31021 attgcaccat tgctcagacg gatatgctta cccgtgagcg tgatcccgaa ctgctgggcg
    31081 aaatcaataa tatattcacg gaagatttcc gcctgtaccc gtgacgcaga aaagaaaatc
    31141 tggttatcac cggtcaccac cgcatcttcg aacgcctccc atgcaaaata ccaggtcatg
    31201 ccgatctggc ggctcttgag gatgaaacgg cgctcacggt gccggttctc gcggcagtgc
    31261 agctggtagg cataaagatt tttacgcgcc cattcatcca gcatttcagc agtcagtccg
    31321 gaaacgtcgt ttttccggta gcgccgcttt ttcccttctc ccccgccgca gatccccgca
    31381 gccacgccgt cctcatgccc tgccggacgc agggcggcaa tttccgccat cctttcagcg
    31441 tgtttgtttt tctgggcgat gatcttcacg tgctgtgcga taagttcacg cagttctgca
    31501 agctcacact ctgtcttgcc ctcccgtccg gtcagcacat caatacgccg ggcgatcacg
    31561 tcctcaacgg attcagcggg cagcagggca gcccaccgcc ccacatcaca ccagtggtaa
    31621 accgtgcgcg gcggaatacc gagttccacc gcaatatcgc gggggtgcca ccgcttcagg
    31681 taaagcgatc gcgccgcctc tttgatttca tcagaatatt tagccatgcg cccattatgg
    31741 cggcgcatcc ggcccgcatt caggcagaaa atttcggctg tgttcggcta tcccgcgtta
    31801 tccgaagaca gccgaattca gccagtagcc ccccgctgta aaaatcccga cactcaccgg
    31861 agttcttcag attttcacgt aaaagaggcc acgatgccgc agccaacgtt gatgaccgac
    31921 tggatatgta tcgccacatc aggaccgacc gttgacggcc gcgaaattga tccgcagtgg
    31981 ctgactgatg ccgctgaaac ctattcccgc aacacttaca cggcgctgct gtggccctgg
    32041 catgaggaag aaatcggaca ccgccagtac accagtaatc tgggtgaagt ggactcactg
    32101 aaagcggaaa ccggcgagga cggtaaaacc cgcctctacg cacggctggt ccctaaccag
    32161 tacctgattg aagccaacag actggggcag aagctgttca catccgccga gatagtggag
    32221 aacttcgccc ggagcggacg cgactatctc ctgggcgtgg cggtgacgga tatccccgcc
    32281 agtctgggga cggaaaaaat ccgcttcacc ctgaacaacg aggaatacac ggcacagcgg
    32341 gcgaactgcc agacgttcac gctgggcagc ctgtcaccgg acacctcccc gcccccccgt
    32401 aaacctgaat cattcctgaa acgcctgttc agcgcaggac agaccgacaa caccgacacc
    32461 agtaagcctg atacaggcga ggacgcaaaa atggacgaaa ttaaagcact gcttgaaaag
    32521 ctgatcaccc tggtggaagg cggcagcgcc gctgccgctg gcgagggcac agcagacacg
    32581 ccggaagaag caaaagccga agtcgcggaa atcgccgccg aaatcgccga cgccgccgca
    32641 gaggtggcag agctggcaga agaagtgaaa gccgccccgg aagacgaggt taaggccgaa
    32701 gcctttaccg ctgcaaaagc gaagctggaa gacgccatca aagccttcag caccatcaca
    32761 ccggaaaaag gcgaacgtta ccgccgcttt gccgcccgtc gccgggacga caccgacaaa
    32821 gaagaaatca aagccattgc cgaaaaactt aacgacctga ccacgaagct gtcagccacc
    32881 ggcatcacgc cacgcccggc tggcacaccg gcagaaagcg aaaaaccgtt tgactttgtt
    32941 taaggatacg caacatgcaa ctgaaccaga aaaccgaatc actgcttcgt aagtacgctg
    33001 ccgggctggg tgcggcaaac ggggtcgacg acgtcacacg ctattttgcg ctgtctgacc
    33061 cgaaggagac cgcgctgcgt gacgccatca tgcactccga cgagttttta tcaaaactgc
    33121 cgaacgtgct ggatgttgaa caggtgacag gccaggtggt taccaccggc gttccgggcc
    33181 tccataccgg acgtaaaaaa aatgcccgct tcacccttcc gctgggtgtt accgggaatg
    33241 aatacaagct ggctgaaacc gattccggtg cccacctcct gtacaccaca ctggtcacct
    33301 gggcaaacgc aggcggtgaa aacgagttct ttaaccgcat tcagaatttt gttaacagac
    33361 agatcgcgct ggatatcctg cgcattgcct ttaacggtac cagcgtggcc gaagataccg
    33421 acccggtaac caacaaaaac ggcgaggacg tcaacaccgg ctggcatgcc atcgtcaaag
    33481 gccgcagcgc aaaacagatc gtggaggctg gcccgacgct gggcaagggt ggcgacttta
    33541 tcgggctgga tgcagccgtg gcagacctcg tgcacaccct cattgatgag tcgtatcgcg
    33601 gtcatccgga tctggtggta ctggttgcgc ccgatcttgt ggcacaggac aaggtgacgc
    33661 tgcttaacgc catcgacgcc cccacggaaa aagtggccgc gcagcttatc ggtcgccaga
    33721 tcgccgggct gacagcatac accccgccgt tcatgccgga aggacgcctg attgtcacca
    33781 cgctggcaaa ccttcacatc tacacgcagc gcggcacccg tcagcgccgt gcggagtgga
    33841 acgacgatcg caagcgtttt gaaaacaact atctgcgcat ggaaggttac gccgtggagc
    33901 acgacgaaat ttacgcggcc tacgacaagc tgaccatcaa gggcgcagaa gcggcagaag
    33961 agcccgcatc tggcggcgca taagcacaga gggcggcgca atgacaccct gccagcgaca
    34021 ccgggcgaaa atccgcacac aggaggcact cgaacgccgg gaggcgctga cggcctcccc
    34081 ggtcagcttc cacctgttac gcgcggagct tgatcgcgat gtggcacggc tgcgcagtct
    34141 tccggtccgt gaagagcggc tggcgttcaa gcgcgacatt ctcctgccgc gctggctgcc
    34201 cgtcgcggag cgctatatcg ctgacggaaa gcgccacgcc tgcccggtac tggtgtactg
    34261 catcatctgg ctgttcgaca cgggcgacct gagtcgcgcc cttgactggg cagacattgc
    34321 catcagtgaa ggccaggcca cgccggacaa cttccgtagc accctgcccg ccttcgtggc
    34381 cgacacggtg ctgcagtggg ccaccgacaa cagcgaggcc ggacacagca tagaaccgta
    34441 tttttcgcgc accttcagga acgtcaccac cacgtggcgg ctgcatgagg aaatccgcgc
    34501 gaagtggttc agatttgccg ggctgtatct tctgcgcgac gaggcgggca aaccgagagc
    34561 caccgcgatt aatgatactg ccgtactgga aaaggcagac gcatacctgg cacaggcggc
    34621 agcactgagc cgtaccgctg gcgtaaaaag tatcagggcg cgcatcaggg cacgcatcag
    34681 cgcccttaat gcagcgtaaa cgactacccg cagggccggg cgggcgcggg aggaggcatc
    34741 agtgacggaa actgtatggc cgtggaatcc cggacagccc gcctttttat tcatatgagg
    34801 cacgtcatga gcgaaccgag cttcagcgtc agcggtaaac cgctgaaatc cacaccgcag
    34861 acggtgacaa acggcgagcc gttctggccg gaccttgatc tggctgagct tcaggggtca
    34921 cgcacgcttc cggctgattt acccgacgac acggcggtca tggctctgct tgccgcagtg
    34981 gccgaggtaa acaccacgct ggcgccggtt gtggcgcact ggaaggcgca ggggcatgaa
    35041 cgcgccgcag acgtaccggg ggccaggctg ggcgatgaaa cccagctggt cgcacagtac
    35101 aaaaaggccg tttatgcccg tgccaaggcc gatcttatgg gcgagttcgc caccattggt
    35161 cgccgtgaaa cctatccggg gcaggaaagc gaggacacac gcgccacctt actggctgaa
    35221 gcctcgctgg tcatgcgtaa catgctgggg cacagacgcg cgggagtgca catcatatga
    35281 accagctgga aagcctgacc gccttcatta ccgaaaatct gccgcctgac gccatgcagt
    35341 tcttcatgag cagtcaggaa gacgcgcagc ttatccgcag tcagaaggcg ctgggtaacg
    35401 accagatccg catgggcgtg atgacgtaca ccggcgtgat tgcatgggat aacttcccgt
    35461 tccgcaaata ctcaccgggg ctggtttacg cgctggtcat ggcctggctg gaagagcacg
    35521 cggacccgct gagggaggaa ttccgcccgg aggatgtgaa cgtcgacccg cagtttaacg
    35581 atgaggactc gtgccttctc gatatcacgg tgggactggc tgacccgctg atcatccggg
    35641 tgcaggaaga cggccccgtt ccgtacaggg ggaaacgctg gacactggtt aaccctgaaa
    35701 tctggacggc ggaaaccatg gagctgtttg tcagcaggag ccgcgaatga taagcggcca
    35761 gctgaacccg cagcagctgg cagaagcgca ggcgatgctt cagtcgctgg atctgccacc
    35821 aaaaaaacgc cgtcgcctgt tatggcgtat tgcccggcac ggcattatcg ccgcagccag
    35881 acgccatcag cgcgatcaga ccgcgccgga cggcacagca tgggcacccc ggaaacgcag
    35941 caaggggaaa atgttcagga agctgccgcg tcttctggcg gtccgtgaga tgcccggaca
    36001 ggatgccgta aaggtttatt tccgtaacgg caatcacacc agtggcagga gccgcatcag
    36061 tgccggtgcc gtgggctggg ttcaccacaa cggcgcagaa atccgggtca gtgctgacag
    36121 cttcagcggt caggaccaga acgggcagac cgccacccgc cgacaggcaa aacgactgcg
    36181 ggagctgggc tttaaggtac gccagaaggg gcgctggcgg aaagcatcca tgaaatacct
    36241 gttaacgctt tcgcgggcgc aggccgggtt gctgatcacc gttatggaag atcagattat
    36301 cgccggacca cgccagcaga aggcccggac atggaccatt acccttccgt cgcgcgtctt
    36361 tctgggggta acaaacgaag aattttcaca catcctggca cgccagatga acgcaatggg
    36421 ctacggcctg gacgttgacg cacaggatat cagataagga gtcacaccgt gagctggccg
    36481 aatgtaaccg taagccatca gaaccgtttc aacggtgcca cccgtgaagt ggagcgcacg
    36541 ctgctttttg tgggctatgg caaaaagaac acaggtaaca ccctttccgt ctcccctgag
    36601 accgacctgg acgacgtgct gggaccggat gaaagcctgc ttaaatccac gcttaccgcc
    36661 gcaatcgcca acggtggcca gaactggttt gcctatgttc acgttctgtc tgaacccaaa
    36721 cccccggcac cggagggcgg cgacgctaac gccgcatggg tggacgcggt gaaaaaagcg
    36781 cagaccattg cttctgccga gggcgtggtg attgccattg acatcacggc caaagatgcc
    36841 gttaaccgcg ccaccgaaac ccgcgccctg cttcagtccg cttatggccg ttttgtctgg
    36901 ttcatgctgt gcgtggccgg accgggcagg gatgaagcat gggcggcata cgtcacccgc
    36961 atcagtgcca tacaggacgg tgtggcagca cctggcgtga tggtggttcc ccgcttatgg
    37021 ggaaatgagc ccggcgtact ggcgggccgt ctctgtaatc cttccgtcac cgttgcggac
    37081 agtccggcac gtgtggcaac cggtgccctg gtggcgatgg gtaacgatga gatcccgcag
    37141 gacggcaaga aacagcccct tgaactggcc acgctgcgct cgctggaagg caaacgcttc
    37201 agcgtgccca tgtggtatca cgatttcgac gggctctact ggtcagacgg acgcaccctt
    37261 gatgtggagg ggggcgatta ccaggtcatc gaaaacgtgc gcgtggtgga taaagtctcc
    37321 cgccgtgtac gtctgcgcgc cattgcccgc cttgcggatc gcgcactcaa cagcacaccg
    37381 ggcagcattg ccgccagcca gacatatttc agccgcacgc tgcgtgaaat ggcgctcgcc
    37441 acggagatta acggcgtacc gttccccggc gaggttaaac cgccacagga cggggatatc
    37501 accatcgtct ggaccagcag tacacacgtg cagatttaca ttgtggtacg cccctacgag
    37561 agcgcaaaag ccattggcgt aagtattgaa ctcgacacag cactggagat ctgacatggc
    37621 cggaaatgaa cgtatcagcg gcggcgcgtt tgatatcaac tacgacggga tcatgatcca
    37681 cgtggaaagc gccaccgtca ccatcaccga caacagcgcc gtggcacaga cgcgcggcgt
    37741 gccgaacggc tacacccgtg gttctgtttc tgccgatgtg gaggtggaag tcgacgcgca
    37801 gaactttaaa aagtttggtg ctgtggcccg ctctgcgggg tcgtggcgtg ccattcctga
    37861 aaaggatttt ctcttttatg ccaacaacgg cagcgaggag gaaaaaatcg aggtgttcgg
    37921 ctgcgtcccc atgttgtcgg acgtggtgaa catcagcgca ggtgaagccg gaaagaccac
    37981 gaaaaaaatc aagttcatgg tcacaagccc ggactttatc agcattgacg gcgtgcccta
    38041 cctgtcagcc cgtgacaccc gcgatctgaa ggggtgatgc catgccggac ggtcagactt
    38101 cactcctggc aaaactgatt atcatcggtg ccgccatcgg gctggggcgt ctcatggtga
    38161 gccgcgatcg catcacagcc cgtctgctta tcgggcgaat gattatgggc tctgccgtgg
    38221 caccggttgc cgccattccg ctgtttaaat ttccggacat gccggagctg gtagtggtcg
    38281 gtctggcgtg cgcgctgggc attatgggca gcgcctttat tgaagaatgc ttttgccgtc
    38341 tggcagaccg ctacctgaag cagaaggaac gcagaaatga cccataagcc ttacatttcg
    38401 ccttccctga gcctgtatca cgcgcaggcg catgacgttc tgcggatgct gccggaaaac
    38461 agtgtggacg cgctcatcac agacccgcca tacagttcag gtgccacaca caaggcgggg
    38521 cggacatgcc agggcagcca cgccaaatac atcaacgatg aagatcttca ccgctttgaa
    38581 ggcttcgaag gcgagaacat ggacgcccgt tcatgggaat actggacgca tctgtggatg
    38641 acacaggcat ggcgcacggt acgacccggt ggttacgccc tggtcttcac cgactggcgg
    38701 cagttgcccg cgctgacgga cgcattccag gccgccggct ttacctggcg ggggatcgtg
    38761 gcatggaaca agggacgggc ctcaagaaca ccgcataccg gttattttcg tcaccagtgc
    38821 gaatatgtgg tctggggcag taaaggcgcg ctgggcaaat ccccttccgg gccgttcgac
    38881 ggctgcatca cgcaccacac acgaccacag gagaaacagc acccgacagg taaaccggaa
    38941 tcgttgatgg atgagcttgt gcgcgtgtgt gaacccggag ggctgatcct cgatccgttt
    39001 atggggtccg gcaccacggg cgtggccgcc cttcgccggg gctgccgttt tacgggtatt
    39061 gaactgaacg cccactattt cagcgtggca caggcgcgcc ttacggggac ggaacagagg
    39121 aatgactgat gaaactgagc gaaaaacaac aactgtttgc cgtcatgatt gccgatctca
    39181 tccactgggc acaggagcac ggctatcgcc tgaccttcgg cgaggcatac cgcacgccgg
    39241 agcaggccgc actgaatgcg aaaagcggaa aaggcattca caacagcctt cacacgctgc
    39301 gcctcgcggt ggacttcaac ctgttcatta acggcgaata ccaggccgac accgacgcct
    39361 acaggccgct gggggaatac tgggaatcca tcggcggcac atggggcggg cgcttcagcc
    39421 gggcggacgg caatcatttc agccttgagc acaacgggat gaaatgatga aacagctggc
    39481 gggctgcctg gtgatcctct ttgccgccat gtgggcaggc tggctggccc atgactggca
    39541 tgacgccagg gtggcacttg ccgaacagga agccatggaa gccacgcgac gtgcggtgac
    39601 agaagccggg cagcagtccg cccgcatact ggaaaaacgg ctggaggcgt taaagggcaa
    39661 tgaaattcac actgaacgcg ttattcacac ggaaaccgtc cgcccggtat tccgcaatgt
    39721 gtgcgctact gatgattacg tccggctgtt caacgcccgt gctgaagccg ctgaacgtac
    39781 cctggcagga aaaccagctg acggaatgcc cgaagacact gccgagactg gcaggcccga
    39841 cgggcgctga ttttgatgcg gcactgcgaa ccctcacgag ccgctacacc gaatgcgccg
    39901 ccaggcataa cagcctggtg gaaaccatac gaaacacgga gaaactgcaa tgaccacgaa
    39961 gaacaccggc accatcatcc tgtccgttgc gggcgtaacc ctgagctttg aaccgaacaa
    40021 aacggcattt aacaacctca tcaacgaaat cacgatgacc aacaaggtgg cgccgatgag
    40081 cacctatctt aaccgcatcg tgacgcctgc cagccgcgac gcactggctg acctgattgc
    40141 gcgttatccg ggctgtgaga tgcagatcgt cgagaagatt aacgaggttt actccccgaa
    40201 actggagatt gagttaaaaa actgaccgcg cgggtcgaaa gcatccgccg caatcccctt
    40261 gagcagtatc tggcattacg gcgtcactac ctccccggag agccggacga cgaagagagc
    40321 atcgcccggg ccatctggct ggatgaatat tttgcccgta cccgcgccaa caagaccgct
    40381 gaaggtatag cgatcgcatt taacggaagg tgacatggcc agccagctgg atttcattat
    40441 caatctgaca gaccagctta cccgcccgct gcggcaggtt caggaatccg tgacgggatt
    40501 cgccgaacgc tcgcagggcg cgttcgcgcg tatcggtgtg ggtgtggccg gactgtgggg
    40561 cgtggccgaa ggtatccgcg ccgcgatgga tccggcgtgg gagatgcaga aggcgctggg
    40621 cgagcttgag gccaaaggcg tcggggcgca ggcgctgacc gccctcacca cgcaggccat
    40681 gaaactgggg accgcattcg gtcagtcttc cacggagatc atccgttccg catggcatat
    40741 tcgcggcgca ctggacggac tgagcgacgg ggatttaccc cgtgtgacgg cagcaaccag
    40801 caccctggcc gtggctgccc gcgtcagcag cgaagaggcg agcggctacc ttgccgccat
    40861 gggtgcccgt ttccgcagtg agttttctgc catgggtgcc gtggggtttg ctgaagatct
    40921 ggcaggtaaa accgcgtgga tggttcagca gttcggcgtg gaaatgagcg ccatgcagga
    40981 gcttattgag ggcaccaaaa acgcgggcgc ggattttggc gtcagcatgg cagagcagtt
    41041 tgccatactg ggcaccctgt cacggaccat gggcaccgag gcatcgggca tctatgagga
    41101 attcctgcgc agtgcccccg ctgccgcaca aaaaatgggg atgagttttg ttgatgccac
    41161 cgggaagatt ttacccatgg gtgacatcct agaccgtctg caggcgaggt tcggcaggaa
    41221 catcgagggt aacgtggcgg cacaacaggc gctggatgac gccttcggca gtggcgcgga
    41281 cgtgattaaa aaactctggg gacagcagga aatgcttaac cggagcatat cagcgctggg
    41341 gcgtaatgac ggcatgaagc gtgccacgga aatggcggaa gccatgactg acccgtggga
    41401 ccgccttgtg gcctccttct acaacatccg ggccgccatc ggtaacgccc tctaccccat
    41461 tctcgcacca ctggctggcc gttttgccga tatcggcgcg acgttcgtgc gctggctgaa
    41521 catgttcccg aacatcgccc gctggctggg ttacatcacg ctgaccatgc tggcgctggg
    41581 cgcagccggt gcggtcacga acatcgtgct gggtgtgttc agcttcatca tggaaggcca
    41641 taaggccatc ataaagggcg ccagggcagc ctggagtctg tacacgcttg cactcacaaa
    41701 agtacgcagt gccatcattg ccaccagtct tgccgcgcgt ctggcgggcg tgtcgtttct
    41761 gaccatgagc gccccggtcc tcctggttgc cgccgccatc gctgctctgg tggtggtggc
    41821 cattaagttc tggcagccca tcaaagcctt tattcgcggc tttattgatg gttttaccag
    41881 cgcactggac gccatgagcc cggtgactgc cgcgttcggt gacattgccc gggcgctggc
    41941 cccggtatgg cagggcataa aaacactgtt cagtgccttc tccgatctgt ttgcaccggt
    42001 acagctgacc acgggacaga tgaacagcgt cacccgtgcc ggggaaacat gcggtcgcgt
    42061 ggtggccgca gcggtaagca tgatatcccc cgcgctggag gtggttatca gcgtgatcag
    42121 aaccctgatc gacatcttcg ccatcgtcat cagagggtgg caggacgtgg tggctgcatt
    42181 tgacccgacc gccccggtcg aatccttccg gaaaatggcg gggatcatcg gtaacgtctt
    42241 cggggactta tggaacgtca ttaaaaactc gtttaaccgg acgtggaact ggcttgcgga
    42301 aaagctgaac aaaatccccg gtatcagtat cgacatgaag cccacggagc aggtgaacac
    42361 catgacgcgg ggcgcaacag gcagcaccgt acctccgcag attgcagaaa ccgccgtccc
    42421 ggcactggca ggcaaccgca tcagcgcaga gctgccaccc ggaggcatac gcaaccagat
    42481 acgcacggac acctcaaccc gtatcgacaa cagccggacg ttcggcaatc tgacaatcaa
    42541 cacgcagaac ccgatgacgc cggggcaact ggatgaatgg acggagcttt acgcgggatg
    42601 aacacgccat cgctttacat cgacctgctt atcactgacg gcagttttac gctgaacagc
    42661 ggcaacgagc cacagcgatg tgataaccgg gtcagcatcg gccaggacgt ggtgcacagc
    42721 attatcgaaa gcgggatcgc cgccctgctt atcggcgaac gcagcccgac gctgcgcagc
    42781 gacatcatta cccggcttat cctcctggtt gaggatgatg aacggctggt tccggggacg
    42841 gtgttcatta acgaagaggc accgggcagg ctgcatgtga cggcagagac acaggatttt
    42901 ggacgcataa acaccagggt gaactatgaa agggaaaccg gacgttgatt ttaatcaggt
    42961 attccgtgaa agcggtatac cggaaacaga agaagagatc gccaccctct ttcatgagga
    43021 tgtacagaaa accggactca tcaccaacac ctcgcccatg agtcccttct ggcggctcat
    43081 tctgaccatt gtcatacgac ccgttcagtg gctgcgtgat gcgctggttg acgttgtgct
    43141 gcccggtatg tttgtcgcca cggctgaccg gtcacttctt ctgcttcatg cgcacgacgt
    43201 caacatcacc ccaaaaccgg ccagtaaggc cagtggcgtg atctggtttg ccaaaaccag
    43261 ggcaaatgat gacgtcctca tccccgaagg caccatcgtc cagaccgcca gaattaacgg
    43321 cgtgatatac agcatgaccg tcacggaaga aacgcttatc agcagcggta aaagcggcgc
    43381 atccgttccc gtggaagcca ccaccgaagg cagcgggcac aacctcgcgc cggggtactt
    43441 ccgtatcctt cccgttgcca ttcccggcat tgcatccgtg gtgaaccggg aggactggct
    43501 gaccgtaccg ggtgccgatg aggaaagcga cgacgaactg cgcgacagaa tacgcaacca
    43561 gtttaacctc gtgagcagtt accacacgga cgccgtctac cggggactta tcgccagcgt
    43621 ggcgggcctg agcatcgacc ggatctactt tcagcacgac gccccgagag gaccgggaac
    43681 ggccaacgcc tacctgttac tggacagcgg cgaggcctct gcccctttca ttgaagcagt
    43741 caaccgctac atcaacgaag aggggcacca cggacacggg gatgatttgc agtgtttcgc
    43801 catgccggag acgcgtcacc gtctggaggt caccgtatac atcagaagtt ccaccaccct
    43861 gccaccggac ggcgcggaag ggctgaagca ggatatcggc gacctgatac gctgcgcttt
    43921 ccgggagaac gcccgcttta acgtgaaaaa agtctggccc tattcgcgct tttcgttttc
    43981 cacgctgggc aaggaaattc atcagacctt cccggtggtg gcttcggttc agttttcact
    44041 ggaagacatc atcagcgatc tggccgtgcc acggctcgaa tccctgacag tggaggtggt
    44101 cattgaatga ctttttgcag cggcttcgct cgctgaaact gccctcctgg atggacaggg
    44161 gggaacccga caaactgctg cgggccgcgc aggcgttctg gtcccgcgta tgggaatgga
    44221 tcacctggcc cgtgcgccag tttgacccca tgacatgtgc agagcccgta cttgatttac
    44281 tggcatggga gcgggacatc acccgcttta aaggagaacc cacggagatc taccgccgcc
    44341 gcatcagtta cgccttcatc aatgcacagg aggctggcga ggtggcgggc tttatcgcca
    44401 tttttgaacg gctgggcatc ggctacgtgg agttactcga gcgccagccc ggcgtggact
    44461 gggatgtgat cctggtgcag gtgacagaca gccagatagc cgacaacagc gatcttcttc
    44521 tggagatcat ccgcaaatat ggcaggacat gccgccgtta ccgttttgag gtggaaaccc
    44581 tcaacccctt catcatccat tatggcgcat acgaagggga gtatatctgc tggacggcca
    44641 gggcagagtg aatgactgaa ggagtatgac tttatggcga aatctgtaat taccagcgcc
    44701 tttatccggc tcaaggcaca gcaggcggcg gaaggcaccc ccgttgtgct ggatgagttt
    44761 atttttgcaa acgtcccgga cctcaacccg gaaacaccgg tatcccccga cgaaactctg
    44821 ccacctgaag atcagattgt tcaccgccag gccgttggca aaaccggcgt ggtgaatgag
    44881 aatgccgtgg tttattcggt caccctgggg gcagagctgg gtgattttga atttaactgg
    44941 gtgggactgg tcagcaaagc aaccagcacg ctggccatga ttgttcacgc ccccacacag
    45001 aaaaaaatca gaacccacaa cggtaacgaa ggtaatgtgc tggtccgttc catgctcatg
    45061 gagtacagcg gtgcgaagga agccacaggt atcaccaccc cggcgcagac ctggcagata
    45121 gacttcacgg cacgcctggc atccatggac gaacgccagc gccgcgaaaa cgtcgatatc
    45181 tacgggaagg caactttttt tgatgacggg tggctggtta agaaaggcga aacctggaca
    45241 gtcaacaaag gggtgggtta cgtggagggg ctacgcgcag agctgagcac tcaccacccc
    45301 atgaccacgc ccgccttccc gtcccgtatc tggctgaatg tgtggtggac cggcacgctc
    45361 accagtgaat gggcgacaga gcacgaaatc ataacagccg acccggacgt tacggaagag
    45421 cgtttacgga aaacgtatcc gagggacggc attcagcaat atttttgtgc catcgctgaa
    45481 attgatgaag acggcaacgt gactgacctc cgccccagag gaacgccgga agagcgccgc
    45541 agtctggacg ccctgaaaaa acacgagaaa tcacgcaacc acccggacgc aacgctgaaa
    45601 gaaaagggct tcacacagtt aagcagcgcc accaccagta catcagagac acaggcagcc
    45661 acgccaaagg ctataaagat cgccatggac aacgccgatg gtcggcttgc gaaaaaccgc
    45721 aatggtgagg acatccccaa caaggagctg ttcctgatca acgtgggcgc accacgcgtt
    45781 tatgcaaaag acgggtacgt gggtaaagca gggcaacgct ggacaacgga acaattcgtg
    45841 gaatggctga atgaacgtgg cgccttcaga ccaggactct ggatattcaa agccaatatt
    45901 tcactgtcac agcacagcgt gctcactgat acgggctatg gcgatatctg ccttggaggc
    45961 tgccttatcg aagtatcagg cgacatcaac ggggggctca tcaggatcac tacaggcatt
    46021 gaatcccctg aagacatcac cagacctcac cgcacacagt tcaccggagt tattttaagt
    46081 aaagggaaaa gcgccatctg gtaccgcact tacacggaca aaaacaaacc cacaccacag
    46141 gacatcggcg cactgcctgc aagtgagttg ttacccgcag gtgttcccct gccgtggccg
    46201 tcagacaccc cgcctgacgg ttacgccatc atggtggggc agacattcga taaagcgcaa
    46261 tatcccctgc tggcaaaagc ctatccggac ggtcgcatcc cggatatgcg cggctggaca
    46321 atcaagggaa aacctgccag cggtcgtgcg gtattgtcac aggaacagga cggcatcaaa
    46381 tctcacaccc acggggggag tgtctcaaat actgaccttg gcacgaaaac gacctcatca
    46441 tttgattacg gaaccaaaac aacctctacg aacggacaac atgaacacag cgtccccctg
    46501 agaagacaag atggcggagg cataaacttt gactggctgg atggttcatc gtctggttct
    46561 catattggta atggatacgt tcctgccgca ggtgaccacc aacataccgt acccattggc
    46621 gcacacatcc acacgctcgc actgggtcaa catggtcacg gcctgaccat taaccccacc
    46681 ggtaatgccg aaaacaccgt taaaaacatc gcatttaact acatcgtgag gcttgcataa
    46741 tgacctttaa aatgagcgaa cacccacaga caattaaaat ctacaacctg tcagccggaa
    46801 caaatgaatt tatcggcgaa ggcgatgcct ggatccctcc gcataccgga ctgcctgcgc
    46861 actcaacgga cattgccccg cctgacattc ctgaaggaca cgtggctatc tttgatgagg
    46921 atgaaaacgc ctggcacctc atggaagatc accggggtaa aaccgtctgg cgtctggagt
    46981 cggggagcgc cttactgatt actgagccgg gcccgttacc ggaaaacgtg accatgataa
    47041 aaccggacgg ggagttctgc cgctgggatg gttcatcatg ggttaaggat gaagcggcag
    47101 aacaggctta ccgggtaagc gaggcggaga ataaaaaacg gaaattaata caggaggcaa
    47161 caaatacaat aaacatcctt caggatgccg tggatttaaa tatggcgacc gacgatgaaa
    47221 gcgccctgct tcccctgtgg aaaaaatacc gggtattact gagccggaca gacacgggca
    47281 acgcaccgga tattgtctgg ccggaatccc cttcgccgga tcatcacgca ggctgaccgg
    47341 aggcaccatg acgtggacaa gaaaaacaat cagcgtcagc gccgacatgc ctccgctgag
    47401 ctgttccgtc atccccgtca caccgtggac ttacgggctg ggacgatatg aagaatcggg
    47461 ggtatattta agccctccga acgccataaa ctggctggcc gggaaaatgg ccggaagccg
    47521 tgcatccggc gacgtgacca ttatcatgat tgccgaaaac acccacgatc tttttataca
    47581 gtcaatggcg gcactgaccg ccgttctgcc cgtgccggta ttcacgcagg cacagcgcat
    47641 ggcacaggct gccgccagtc tgagcactga caaaatgcaa atcccgatca ccaccgacac
    47701 cctgcccgca cctgtgcatc tttccgttgc cacaatgcgc agcgcagtca gtgccgcccg
    47761 tgcagcacag gccaggcagc tggcagcaca acgcccggat gccgcagggc ttcgccgcca
    47821 gataagccgc tttatcagca gacgggctgg cgcacttgcc ggactggaag acgggatcag
    47881 aacactggca gaacagaagg cgcaggcctg ggtatttcag taccgggggt atcaccgcgc
    47941 cgcagcggcg gccatggtga aggatatccc ggcaccgacc gccgtacata ctgtggccgc
    48001 actcctggcg gcagactcat taaccgaact ggggaaaatg atccatgagc cagaccgcac
    48061 tgctcgccct tgatggtgag ggtattttca tgcagaacat ggtgatttcg ccctcgatgc
    48121 agttccagga aaaggaccag tccggccaga catcgagcac cgcgaacgcc gagcagggca
    48181 tcaaggccaa agagcttcgc gtaaccggga tgatcacctt tgacaatgag cgggcccttc
    48241 agcgcatttt tcagctggcc tctgccacca ccggggacgg tgccctgaaa gtttaccgta
    48301 ttgcaaacgc caccgctgcc gccattaact ttcgtgaagg gacattttcc gggcagatag
    48361 acgcacagca gcagaccgac cgcctcgcct ggcaggtcag cttcacatta cgcgaaagac
    48421 gaagcgtacc ggaaaaacgc caggcacgcg ccgcccccgg cagcagccgt aaacagacgc
    48481 cgggaacccg acagacaccc ggcgcaaaag gcaaaaccgt ggccgacgaa acaccggaga
    48541 aaatgacctg gttcgaagaa aaggtcctca aacccgtcaa tgatgcgctg gagtaaccat
    48601 gaaccccact aaacgtctgt tactgggtaa cgacaccatt cagctggtca gttgttccgt
    48661 catgcttgag ctgaacgcgt gcggacgcgg atttatcacg gccagaacag agcagaacta
    48721 caccgggcgc cccgttcgtc tcgatatcgg atacggaaac gacctggtgc gctggtttac
    48781 cggatacgtt gaacgcagcc agcccgctga aaacggctcg gtgcggcttc tgattcgtga
    48841 aatggcgggg atccttgatc accccttccc ctgttcattt cagcacccga caatgcgcac
    48901 ggtcacacag tggctgagtg agaccagcgg gctggaaatc atcctgccgg acaacgcggc
    48961 ttacactgac agacccgttc cgcatttcac acacagcggc acgggctacg aactgctggc
    49021 cagtctgggg cgtattttct ccgtaccgga ttacatctgg catccgttac cggacggcgg
    49081 cattatggcc gctgctgccg cgcaggggat gttttccggt cgccccgtca ccatcccgca
    49141 cgagttcagc caggcatcaa cgggcgggca cagtatgacc ctcccgatga ttcagacgct
    49201 gcgaccgggt gtggaggtga acggtcagcg cctgacgatg gtcatgctgg aagatgacaa
    49261 catgaccatc acgtggacac cgcgcaataa agccaccggc gctcccctcc agaagtcccc
    49321 tttccgtcgc caggctgaaa aggccttccc tgagctggca tcagggcttc acctgcccaa
    49381 actggcacgt gttgaggcac cgaccgaagc cgtcagcgcg ggcaatattg ccgacccgtt
    49441 cagaccccgc tacgcggtga atctccagct gcttgacgag aacggcaacc cggcagcaga
    49501 tacgcccgtc tataacgccg ttcccctccc cgttcccatg gcgggcagtg agtccggcat
    49561 gttccagttc ccgccacccg gaaccctggt ggaagtggga tttgtggaag gccgggcgga
    49621 taagcccttc atccgccaga taatggcaga gggccacaat ctgcccgacg tgaagccggg
    49681 cgagcagttg cagcaacagc gcgacggcgt atcgcagcgc gtgacggtcg ccggagactg
    49741 ggagcgccag acagaccagc ggatacagga gacatcacgc gagcgcgtgg tgagcgcaga
    49801 cgaggaaacg cggagcctga ccagccggga gaccaccata caggcaaccg acacaaccac
    49861 cgtcctgggc gtatccacgc ttcaggctgg cgcggtcacc catatcactg aagagaacta
    49921 cagcatcagc acgggcgggc acatgatggt ggttgccggt acctgtgagc gcgacgtgga
    49981 aggcgacaca ctggacaccg tgggcggcag ccttaccgaa aagattaagg gcgtccgtca
    50041 cagtattgcg caggcgcaga agctggttgg cgatacggtg aagctgggaa acgagggcat
    50101 taatgtactg accatgctga cggaccttgc cgatgtggtg gaagagctgg cagatattac
    50161 agcatcacat acccacccca aaacgggcac gtctccgcag gccgcacagt tcagccaggt
    50221 cgctcaggag tgccgccagc taaaaaataa atattcgcca ataatcgaat agttattcat
    50281 tttaacaggc cgcgcaatgt ggcctttttt atccccccga caataaaaaa tattcatcac
    50341 cggacatcac ccgccacgaa aaaaccgtcc gcagcagaaa aaaagatgga cgaaacgccc
    50401 cacgtaatcc ccacgtaaaa cgtcgcgtaa cgccacgtaa cgaagagata accaagacag
    50461 cctgattcga tcccctgaca tcggaaaacg atttctgcgc cccaaaatcg ccgcaaatgg
    50521 ccggaaattt tcgggggcga cgtaacgcgt aaagtgacgt aatcggcgct accccgcaca
    50581 cgcctccggt atttgtgtac gataaatttt gcaaatcgaa aggggtgcaa aacaccccgc
    50641 cagggcgcgc cattactgag gatttccctt ctgaatggtt ttgcactttg tgcaaggttt
    50701 tgcaaaagtg tgcaaaaact ccggctttct gaggatcccg aaaaatttta atgcactgaa
    50761 taacaagaga tttttaaaat tacgtcacga tcgaagatct gaaaaaggat ccgacgacga
    50821 aaacgaaaaa atcaggaaag ccagaactgg cgcggcctgg cagggagtgg cagcaaaaat
    50881 cacttttgca cacttttgta caactgctcc gaaaaaggat ggcgaagggg aaggaaaaac
    50941 agccacgtca tccgttgcac gtaacggacg taaaaggtaa attacgcgcg ggtgcctttc
    51001 ggctgacggc tggaaggatt acctgaaggc cggatgtgga aaggccccga gtcaaacatt
    51061 tgtttaaccc gaggccctaa cccatctacc tcgcaagtgg aaggttagcg cctccctgta
    51121 aaaggagtca agcgctatgc cgcaaaaaac gatcatagtg agcattttat gcataactat
    51181 tttgatgatt gtctggatga tacacaagtc accgtgtgag ttcaggctta acatcatgtg
    51241 gtcacaattt gcggcgttct tgcagtgtaa acagtaagaa accgcagcgg gggtgaaagc
    51301 ccccgctatc cggttgctgg gttcggctga tatggcaccc ttttgggtta gcgtactgac
    51361 ttcattgccc gcaatatgcg ggcttttttt gccgtctccc ctcaacagct tcaatactga
    51421 gcattgcgca cacaaaggga aatcagtaaa ttacatgcgg gtgcctttcg gctgacggct
    51481 ggaaggaata cctgaaggcc ggatgtggaa aggccccgga gtcaacttta atgttaaccc
    51541 gaggccctga ccatctaccc taagcaagtg gaaggttagc gcctccctgt aaaaggagtc
    51601 aagcgctatg ccgcaaaaaa cgatcatcgt gagcatttta tgcttaacca tgttgttgac
    51661 cgtctggatg atacaccgtt ctccgtgtga gttcaggctt aacatcatgt ggtcagaatt
    51721 tgcggcgttc ttgcagtgta aaccgtaaga aaccgcagcg ggggtgaaag cccccgctat
    51781 ccggttgcca aaggttcggt cgaaagtcgc accctcacac aaaagtcaca aagaaaaaca
    51841 aagcccgcgg cgtaaaaaac tgcgggcttt tttgtttcaa agatggacgt tatctggaca
    51901 catgaaaata aaaaatcttt attttcaata ttttaacccc acactaaaac cacccgc
//
LOCUS       DAZUCC010000008        50187 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZUCC010000008 DAZUCC010000000
VERSION     DAZUCC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.008"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            282..1229
                     /gene="cyoA"
                     /locus_tag="ACHY09_000391"
     CDS             282..1229
                     /gene="cyoA"
                     /locus_tag="ACHY09_000391"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFS3883409.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1251..3242
                     /gene="cyoB"
                     /locus_tag="ACHY09_000392"
     CDS             1251..3242
                     /gene="cyoB"
                     /locus_tag="ACHY09_000392"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFS3883410.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3232..3846
                     /gene="cyoC"
                     /locus_tag="ACHY09_000393"
     CDS             3232..3846
                     /gene="cyoC"
                     /locus_tag="ACHY09_000393"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFS3883411.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKVFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVVSWL
                     ALTWLFGAGFIGMELYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3846..4175
                     /gene="cyoD"
                     /locus_tag="ACHY09_000394"
     CDS             3846..4175
                     /gene="cyoD"
                     /locus_tag="ACHY09_000394"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFS3883412.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4187..5077
                     /gene="cyoE"
                     /locus_tag="ACHY09_000395"
     CDS             4187..5077
                     /gene="cyoE"
                     /locus_tag="ACHY09_000395"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFS3883413.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5226..6590
                     /gene="yajR"
                     /locus_tag="ACHY09_000396"
     CDS             5226..6590
                     /gene="yajR"
                     /locus_tag="ACHY09_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3883414.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILAATGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADTAANEALEVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6718..7209)
                     /gene="yajQ"
                     /locus_tag="ACHY09_000397"
     CDS             complement(6718..7209)
                     /gene="yajQ"
                     /locus_tag="ACHY09_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFS3883415.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7377..8288
                     /gene="panE"
                     /locus_tag="ACHY09_000398"
     CDS             7377..8288
                     /gene="panE"
                     /locus_tag="ACHY09_000398"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS3883416.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGITVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8251..8841
                     /gene="yajL"
                     /locus_tag="ACHY09_000399"
     CDS             8251..8841
                     /gene="yajL"
                     /locus_tag="ACHY09_000399"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFS3883417.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8895..10343)
                     /gene="thiI"
                     /locus_tag="ACHY09_000400"
     CDS             complement(8895..10343)
                     /gene="thiI"
                     /locus_tag="ACHY09_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HFS3883418.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10549..10791
                     /gene="xseB"
                     /locus_tag="ACHY09_000401"
     CDS             10549..10791
                     /gene="xseB"
                     /locus_tag="ACHY09_000401"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS3883419.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10791..11690
                     /gene="ispA"
                     /locus_tag="ACHY09_000402"
     CDS             10791..11690
                     /gene="ispA"
                     /locus_tag="ACHY09_000402"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFS3883420.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLEALERIHRHKTGALIRAAVRLGALSAGDKGRRALSVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            11715..13577
                     /gene="dxs"
                     /locus_tag="ACHY09_000403"
     CDS             11715..13577
                     /gene="dxs"
                     /locus_tag="ACHY09_000403"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS3883421.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEIVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            13632..14606
                     /gene="yajO"
                     /locus_tag="ACHY09_000404"
     CDS             13632..14606
                     /gene="yajO"
                     /locus_tag="ACHY09_000404"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFS3883422.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESNENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(14660..15178)
                     /gene="pgpA"
                     /locus_tag="ACHY09_000405"
     CDS             complement(14660..15178)
                     /gene="pgpA"
                     /locus_tag="ACHY09_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS3883423.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDHNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15156..16133)
                     /gene="thiL"
                     /locus_tag="ACHY09_000406"
     CDS             complement(15156..16133)
                     /gene="thiL"
                     /locus_tag="ACHY09_000406"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFS3883424.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(16211..16630)
                     /gene="nusB"
                     /locus_tag="ACHY09_000407"
     CDS             complement(16211..16630)
                     /gene="nusB"
                     /locus_tag="ACHY09_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS3883425.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16650..17120)
                     /gene="ribE"
                     /locus_tag="ACHY09_000408"
     CDS             complement(16650..17120)
                     /gene="ribE"
                     /locus_tag="ACHY09_000408"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFS3883426.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17209..18312)
                     /gene="ribD"
                     /locus_tag="ACHY09_000409"
     CDS             complement(17209..18312)
                     /gene="ribD"
                     /locus_tag="ACHY09_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFS3883427.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18316..18765)
                     /gene="nrdR"
                     /locus_tag="ACHY09_000410"
     CDS             complement(18316..18765)
                     /gene="nrdR"
                     /locus_tag="ACHY09_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS3883428.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            18916..19455
                     /gene="yajI"
                     /locus_tag="ACHY09_000411"
     CDS             18916..19455
                     /gene="yajI"
                     /locus_tag="ACHY09_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFS3883429.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            19754..20638
                     /gene="tsx"
                     /locus_tag="ACHY09_000412"
     CDS             19754..20638
                     /gene="tsx"
                     /locus_tag="ACHY09_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFS3883430.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20676..21023)
                     /gene="yajD"
                     /locus_tag="ACHY09_000413"
     CDS             complement(20676..21023)
                     /gene="yajD"
                     /locus_tag="ACHY09_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFS3883431.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21153..22124)
                     /gene="secF"
                     /locus_tag="ACHY09_000414"
     CDS             complement(21153..22124)
                     /gene="secF"
                     /locus_tag="ACHY09_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFS3883432.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22135..23982)
                     /gene="secD"
                     /locus_tag="ACHY09_000415"
     CDS             complement(22135..23982)
                     /gene="secD"
                     /locus_tag="ACHY09_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFS3883433.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(24010..24342)
                     /gene="yajC"
                     /locus_tag="ACHY09_000416"
     CDS             complement(24010..24342)
                     /gene="yajC"
                     /locus_tag="ACHY09_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS3883434.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24365..25492)
                     /gene="tgt"
                     /locus_tag="ACHY09_000417"
     CDS             complement(24365..25492)
                     /gene="tgt"
                     /locus_tag="ACHY09_000417"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS3883435.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(25547..26617)
                     /gene="queA"
                     /locus_tag="ACHY09_000418"
     CDS             complement(25547..26617)
                     /gene="queA"
                     /locus_tag="ACHY09_000418"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS3883436.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDERINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            26710..27291
                     /gene="acpH"
                     /locus_tag="ACHY09_000419"
     CDS             26710..27291
                     /gene="acpH"
                     /locus_tag="ACHY09_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFS3883437.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     NSWYDLDAHYDALETCFWQFYPRMMEQASRKAL"
     gene            complement(27296..29113)
                     /gene="malZ"
                     /locus_tag="ACHY09_000420"
     CDS             complement(27296..29113)
                     /gene="malZ"
                     /locus_tag="ACHY09_000420"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFS3883438.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVPMHKQRSQPQPGVTAWRAAIDLSGGLPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLYQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29269..30642)
                     /gene="proY"
                     /locus_tag="ACHY09_000421"
     CDS             complement(29269..30642)
                     /gene="proY"
                     /locus_tag="ACHY09_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFS3883439.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQLTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     SLKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENH"
     gene            complement(30718..32037)
                     /gene="brnQ"
                     /locus_tag="ACHY09_000422"
     CDS             complement(30718..32037)
                     /gene="brnQ"
                     /locus_tag="ACHY09_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFS3883440.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32444..33739)
                     /gene="phoR"
                     /locus_tag="ACHY09_000423"
     CDS             complement(32444..33739)
                     /gene="phoR"
                     /locus_tag="ACHY09_000423"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFS3883441.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKVTRFSFVIPERLIAKNSD"
     gene            complement(33797..34486)
                     /gene="phoB"
                     /locus_tag="ACHY09_000424"
     CDS             complement(33797..34486)
                     /gene="phoB"
                     /locus_tag="ACHY09_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFS3883442.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            34676..35878
                     /gene="sbcD"
                     /locus_tag="ACHY09_000425"
     CDS             34676..35878
                     /gene="sbcD"
                     /locus_tag="ACHY09_000425"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFS3883443.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            35875..39018
                     /gene="sbcC"
                     /locus_tag="ACHY09_000426"
     CDS             35875..39018
                     /gene="sbcC"
                     /locus_tag="ACHY09_000426"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFS3883444.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISSMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIKEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSVREHLRQWQQQLTHA
                     EQKLNALTAITLTLTADEVATALAQHAEQRTLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTMEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            39144..40340
                     /gene="araJ"
                     /locus_tag="ACHY09_000427"
     CDS             39144..40340
                     /gene="araJ"
                     /locus_tag="ACHY09_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFS3883445.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGMVLGNVLSGRISGRYSPLRIAAVTD
                     FIIVLALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQ
                     IAFNLGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPV
                     LAKPLG"
     gene            complement(40479..41387)
                     /gene="mak"
                     /locus_tag="ACHY09_000428"
     CDS             complement(40479..41387)
                     /gene="mak"
                     /locus_tag="ACHY09_000428"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFS3883446.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            41512..42423
                     /gene="rdgC"
                     /locus_tag="ACHY09_000429"
     CDS             41512..42423
                     /gene="rdgC"
                     /locus_tag="ACHY09_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFS3883447.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(42504..42584)
                     /locus_tag="ACHY09_000430"
     CDS             complement(42504..42584)
                     /locus_tag="ACHY09_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883448.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(42581..42910)
                     /locus_tag="ACHY09_000431"
                     /pseudo
     CDS             complement(42581..42910)
                     /locus_tag="ACHY09_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(43067..43351)
                     /gene="ppnP"
                     /locus_tag="ACHY09_000432"
     CDS             complement(43067..43351)
                     /gene="ppnP"
                     /locus_tag="ACHY09_000432"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFS3883449.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSLFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(43423..44100)
                     /gene="aroM"
                     /locus_tag="ACHY09_000433"
     CDS             complement(43423..44100)
                     /gene="aroM"
                     /locus_tag="ACHY09_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFS3883450.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(44358..44549)
                     /gene="yaiA"
                     /locus_tag="ACHY09_000434"
     CDS             complement(44358..44549)
                     /gene="yaiA"
                     /locus_tag="ACHY09_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFS3883451.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(44599..45123)
                     /gene="aroL"
                     /locus_tag="ACHY09_000435"
     CDS             complement(44599..45123)
                     /gene="aroL"
                     /locus_tag="ACHY09_000435"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFS3883452.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(45306..45764)
                     /gene="yaiI"
                     /locus_tag="ACHY09_000436"
     CDS             complement(45306..45764)
                     /gene="yaiI"
                     /locus_tag="ACHY09_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS3883453.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            45884..46693
                     /gene="proC"
                     /locus_tag="ACHY09_000437"
     CDS             45884..46693
                     /gene="proC"
                     /locus_tag="ACHY09_000437"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS3883454.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(46710..47825)
                     /gene="adrA"
                     /locus_tag="ACHY09_000438"
                     /gene_synonym="dgcC"
     CDS             complement(46710..47825)
                     /gene="adrA"
                     /locus_tag="ACHY09_000438"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFS3883455.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(47927..48247)
                     /gene="psiF"
                     /locus_tag="ACHY09_000439"
     CDS             complement(47927..48247)
                     /gene="psiF"
                     /locus_tag="ACHY09_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFS3883456.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(48366..49781)
                     /gene="phoA"
                     /locus_tag="ACHY09_000440"
     CDS             complement(48366..49781)
                     /gene="phoA"
                     /locus_tag="ACHY09_000440"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFS3883457.1"
                     /translation="MKQSTIALALLPLLFSPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATVGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(49881..50141)
                     /gene="iraP"
                     /locus_tag="ACHY09_000441"
     CDS             complement(49881..50141)
                     /gene="iraP"
                     /locus_tag="ACHY09_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFS3883458.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
BASE COUNT        12185 a        13408 c        12701 g        11893 t
ORIGIN      
        1 tacccagttg tcaccttaat gataattatt tgttaaataa ttgttttatt tcgcattggt
       61 tataccaatt gcccgcccag actccttctg cactcccctt ttgttataac gcccttttgc
      121 aacagcttct taaaatcaac ctgatatgtt ttgcaacata tgtgacctgt aagccaaatc
      181 caagtaacag gaatttaatc atgtttacag taatgtaacc ttcccgtaaa atgcccacac
      241 actttaaacg ccaccagatc ccgtggaatt gaggtcgtta aatgagactc aggaaataca
      301 ataaaagttt gggatggttg tcattatttg caggcactgt attgctcagt ggctgtaatt
      361 ctgcgctgtt agatcccaaa ggacagattg gtctggagca acgttcactg atactgacgg
      421 catttggcct gatgttgatt gtcgttattc ccgcaatctt gatggctgtt ggtttcgcct
      481 ggaagtaccg tgcgagcaat aaagatgcta agtacagccc gaactggtca cactccaata
      541 aagtggaagc tgtggtctgg acggtaccta tcttaatcat catcttcctt gcggtactga
      601 cctggaaaac cactcacgct cttgagccta gcaagccgct ggcacacgac gagaagccca
      661 ttaccatcga agtggtttcc atggactgga aatggttctt catctacccg gaacagggca
      721 ttgcaaccgt gaatgaaatc gctttcccgg cgaacactcc ggtgtacttc aaagtgacct
      781 ccaactccgt gatgaactcc ttcttcattc cgcgtctggg tagccagatt tatgccatgg
      841 ccggtatgca gactcgcctg catctgatcg ccaacgaacc cggtacttat gacggtatct
      901 ccgccagcta cagcggcccg ggcttctcag gcatgaagtt caaagctatt gcaacaccgg
      961 atcgcgccgc attcgaccaa tgggtcgcaa aagcgaaaca gtcgccgaac agcatgtctg
     1021 acatggcagc gttcgaaaaa ctggccgcgc ctagcgaata caaccaggtg gaatatttct
     1081 ccaacgtgaa accagacttg tttgccgatg tgattaacaa gtttatggct cacggtaaga
     1141 gcatggacat gacccagcca gaaggtgagc acagcgcaca tgaaggtatg gaaggcatgg
     1201 acatgagcca cgcggaatcc gcccattaaa ggggttgagg aagaataaag atgttcggaa
     1261 aattatcact tgatgcagtc ccgttccatg aacctatcgt catggttacg atcgctggca
     1321 ttattttggg aggtctggcg ctcgttggcc tgatcactta cttcggtaag tggacctacc
     1381 tgtggaaaga gtggctgacc tccgtcgacc ataaacgcct cggtatcatg tatatcatcg
     1441 tggcgattgt gatgttgctg cgtggttttg ctgacgccat tatgatgcgt agccagcagg
     1501 ctcttgcctc ggcgggcgaa gcgggcttcc tgccacctca ccactacgat cagatcttca
     1561 ccgcgcacgg cgtgattatg atcttcttcg tggcgatgcc tttcgttatc ggtctgatga
     1621 acctggtggt tccgctgcag atcggcgcgc gtgacgttgc gttcccgttc ctcaacaacc
     1681 taagcttctg gtttaccgtt gttggtgtga ttctggttaa cgtttctctc ggcgtgggcg
     1741 aatttgcgca gaccggctgg ctggcctatc caccgctatc gggaatagag tacagtccgg
     1801 gagtcggtgt cgattactgg atatggagtc tccagctatc cggtataggt acaacgctta
     1861 ccggtatcaa cttcttcgtt accattctga agatgcgcgc accgggcatg accatgttca
     1921 agatgccagt atttacctgg gcatcactgt gcgcgaacgt actgattatt gcttccttcc
     1981 caattctgac ggttaccgtt gcgttgttga ccctggatcg ctatctgggc acccatttct
     2041 ttaccaacga tatgggtggc aacatgatga tgtacatcaa cctgatttgg gcctggggcc
     2101 acccggaagt ttacatcctg atcctgcctg ttttcggtgt gttctccgaa attgcggcaa
     2161 ccttctcgcg taaacgtctg tttggttata cctcgctggt atgggcaacc gtctgtatca
     2221 ccgtgctgtc gttcatcgtc tggctgcacc acttctttac gatgggtgcg ggcgcgaacg
     2281 taaacgcctt ctttggtatc accactatga ttatcgccat cccgaccggg gtgaagatct
     2341 tcaactggct gttcaccatg tatcagggcc gcatcgtgtt ccattctgcg atgctgtgga
     2401 ccatcggttt tatcgtcacc ttctcggtag gcggtatgac tggcgtgctg ctggcagtac
     2461 cgggcgcgga cttcgttctg cataacagcc tgttcctgat tgcgcacttc cataacgtga
     2521 tcatcggcgg cgtggtcttc ggctgcttcg cagggatgac ctactggtgg cctaaagcgt
     2581 tcggtttcaa actgaacgaa acctggggta aacgcgcgtt ctggttctgg atcatcggct
     2641 tcttcgttgc ctttatgccg ctgtatgcgc tgggcttcat gggcatgacc cgtcgtttga
     2701 gccagcagat tgacccgcag ttccacacca tgctgatgat tgcagccagc ggtgcagtac
     2761 tgattgcgct gggtattctc tgcctcgtta ttcagatgta cgtttctatt cgtgaccgcg
     2821 accagaaccg tgacctgact ggcgacccgt ggggtggccg tacgctggag tgggcaacct
     2881 cttccccgcc tccgttctat aactttgccg ttgtgccgca cgttcacgag cgtgatgcat
     2941 tctgggaaat gaaagagaaa ggcgaagcgt acaaaaagcc tgaccactat gaagaaattc
     3001 atatgccgaa aaacagcggt gcaggtatcg tcattgcggc tttctccacc atcttcggtt
     3061 tcgccatgat ctggcatatc tggtggctgg cgattgttgg cttcgcaggc atgatcatca
     3121 cctggatcgt gaaaagcttc gacgaggacg tggattacta cgtgccggtg gcagaaatcg
     3181 aaaaactgga aaaccagcat ttcgatgaga ttactaaggc agggctgaaa aatggcaact
     3241 gatactttga cgcacgcgac tgcccacgcg cacgaacacg ggcaccacga tgcaggcgga
     3301 accaaagtct tcggattctg gatctacctg atgagcgact gcattctgtt ctctatcctg
     3361 tttgctacct atgccgttct ggtgaacggc accgcaggcg gcccgacagg taaggacatt
     3421 ttcgaactgc cgttcgttct ggttgaaact ttcctgctgt tgttcagctc catcacctac
     3481 ggcatggcgg ctatcgccat gtacaaaaac aacaaaagcc aggttgtctc ctggctggcg
     3541 ttgacctggt tgtttggcgc cggatttatc gggatggagc tctatgaatt ccatcatctg
     3601 attgttaacg gcatgggtcc ggatcgcagc ggcttcctgt cagcgttctt tgcgctggtc
     3661 ggcacgcacg gtctgcacgt cacttccggt cttatctgga tggcggtgct gatggtgcaa
     3721 atcgcccgtc gcggcctgac cagcactaat cgtacccgca tcatgtgcct gagcctgttc
     3781 tggcacttcc tggatgtggt ttggatctgt gtattcactg ttgtttatct gatgggggcg
     3841 atgtaatgag tcattctacc gatcacagcg gcgcgtccca tggcagcgta aaaacctaca
     3901 tgacaggctt tatcctgtcg atcattctga cggtgattcc gttctggatg gtgatgacag
     3961 gagctgcctc tccggccgta attctgggaa caatcctggc aatggcagtg gtacaggttc
     4021 tggtgcatct ggtgtgcttc ctgcacatga ataccaaatc agatgaaggc tggaacatga
     4081 cggcgtttgt cttcaccgtg ctaatcatcg ctatcctggt tgtaggctcc atctggatta
     4141 tgtggaacct caactacaac atgatgatgc actaagagcg gcggttatga tgtttaagca
     4201 atacctgcaa gtaacgaaac caggcatcat ctttggcaac ctgatctcgg tgattggggg
     4261 attcctgttg gcctcaaagg gcagcattga ttatcccctg tttatctaca cgctggttgg
     4321 ggtgtcactg gttgtggcgt cgggttgtgt gtttaacaac tacatcgaca gggatatcga
     4381 cagaaagatg gaaaggacga agaatcgggt gctggttaaa ggcctgatct cacctgctgt
     4441 ctcgctggtg tacgccacct tgctgggtat tgctggcttt atgctgctgt ggtttggcgc
     4501 gaatccgctg gcctgctggc tgggggtgat gggctttgtg gtttatgtcg gcgtttatag
     4561 cctgtacatg aaacgccact cagtctacgg cacattgatt ggttcgctct ccggcgctgc
     4621 gccgccggtg atcggctact gtgcagtaac cggcgagttc gatagtggcg cagcgatcct
     4681 gcttgctatc ttcagtctgt ggcagatgcc tcactcctat gccatcgcca ttttccgctt
     4741 taaggattac caggcggcaa acattccggt attgccagtg gtaaaaggca tttcggtggc
     4801 gaagaatcac atcacgctgt atatcatcgc ctttgccgtt gccacgctga tgctctctct
     4861 tggcggttac gctgggtata aatatctggt agtcgccgcg gcggtgagcg tctggtggtt
     4921 aggcatggct ctgcgcggtt ataaagttgc tgatgacaga atctgggcgc gcaagctgtt
     4981 cggcttctct atcatcgcca tcactgccct ctcggtgatg atgtccgttg attttatggt
     5041 gccggactcg catacgctgc tggctgctgt gtggtaacac aacctctcag ttaaaaaggt
     5101 gctacggcac cttttttctt agcattagaa tcatatccct ctcgaaatat ttactaaaaa
     5161 atccgcatgt ttaccccatt cgtttgccgc tttacactag tcgcgaattt aaaacagagg
     5221 tggtaatgaa cgattataaa atgacgccag gtgagcgacg cgcgacctgg ggtttaggga
     5281 ccgtattctc gttgcgcatg ctgggcatgt tcatggttct gccggttctg accacgtacg
     5341 gcatggctct gcaaggtgcc agcgaagcat taatcggtat tgccattggt atttatggtc
     5401 tgactcaggc cgtttttcag attccgtttg gcctgctttc cgaccgcatt ggtcgcaaac
     5461 cattaattgt cggtgggctg gcagtgtttg ccgccggtag cgttatcgct gcgctctctg
     5521 actccatctg gggaattatt ctgggccggg cgctacaagg ctccggtgcg attgccgctg
     5581 ccgttatggc gctgctttcc gatctcacgc gcgaacaaaa ccgcaccaaa gcgatggcgt
     5641 ttatcggcgt gagctttggc attacctttg ccattgcgat ggtgcttggc ccgatcatca
     5701 ctcacaaact tgggctgcac gcgctgttct ggatgatcgc tattctggca gcaaccggca
     5761 ttgcgttgac catttgggtt gtgcccaaca gtagcactca cgtacttaat cgtgagtccg
     5821 gaatggtgaa aggaagtttc agtaaagtgc tggcggaacc gcggctgctg aaactcaact
     5881 ttggcattat gtgtctgcat attttgctga tgtcgacctt tgttgccctg cccggacaga
     5941 tggctgatgc ggggttcccg gcggctgaac actggaaggt ctacctggca acgatgttaa
     6001 tcgcctttgg ctcggtcgtg cctttcatta tctacgctga agttaagcgc aaaatgaagc
     6061 aagtctttgt cttttgcgtc gggctgatcg tggttgcgga aattgtgttg tggaacgcac
     6121 aaacgcagtt ctggcaactg gtggtcggcg tgcagctttt ctttgtggcg tttaatttga
     6181 tggaagccct tctgccttca cttatcagta aagaatcgcc agcaggttac aaaggtacgg
     6241 cgatgggtgt ttactccacc agccagtttc ttggcgtggc gattggcggt tcgctgggcg
     6301 gctggattga cggcatgttt gacggtcagg gagtatttct cgctggcgca atgctggccg
     6361 cagtgtggct ggcagtcgcc agtaccatga aagaaccgcc gtatgtcagc agcttgcgca
     6421 ttgaaatccc ggcggacact gccgcaaacg aagcgttaga agtacgtttg ctggaaactg
     6481 aaggcgtcaa agaagtgttg attgcagaag aagaacattc agcgtatgtg aaaatcgaca
     6541 gcaaagtgac gaatcgcttt gaggttgaac aggcaattcg ccaggcataa aagcgaggcg
     6601 aaattgcctg ctgcgctacg cttatcaggc ctactaagtt cctgagttac aaacctttgt
     6661 aagccaaatc aggcgttcat gccgcatctg acatgaacaa agcgcaggtt gtcgcgatta
     6721 atcgcggaag tttttgaact ggaacggctg accgagatcg ccaccacgta ccatcgccat
     6781 gacagcctgc aaatcatcac gagatttacc cgttacgcgg atctcatcgc cctgaatttg
     6841 cgcctgcact ttcagtttgc tgtctttgat cattttgacg attttcttct gagtcgcgct
     6901 ctcaatgccc tgtttcagtt tcgcttccac aaaccaggtt ttaccgctat gaacgatatt
     6961 ttccggtaca tccagcgaac tgccttcaat gccgcgcttc agcagcttgg cacgcagaat
     7021 atccagcaac tgattgacct ggaaatcgga ctcgctcaac actttgatgg ttttgctggc
     7081 gtcgttcagc tcaaatgagg cttcaacatt acggaagtca aaacgggact ccacttcgcg
     7141 gctcgcgtta tcgaccgcgt tacgtgcttc ctgaagatca acttcagaga caatatcgaa
     7201 agatggcatc ttttcctctc ccttcatttt tgatgcgaag cataatacct gcaaagttaa
     7261 ataaccaaca gtcctgcacc aggacgtatc ccttaattgt gcatagtcgc aacagcatgc
     7321 acaattaagg gatagcctta ttgaacaccc ggagtggttg cgggtgagga ggaacaatga
     7381 aaattaccgt attgggatgc ggtgccttag ggcaattatg gcttacagca ctttgtaaac
     7441 agggtcacga cgttcagggc tggctgcgcg taccgcaacc ttattgtagc gtgaatctgg
     7501 ttgagacaga tggttcgata tttaacgaat cgctgaccgc caacgatccc gattttctcg
     7561 ccaccagcga tctgctcctg gtgacgctga aagcatggca ggtttccgat gccgtcaaaa
     7621 gcctcgcgtc cacactgcct gtaactacgc caatactgtt aattcacaac ggcatgggca
     7681 ccatcgaaga gttgcaaaac attcagcagc cattactgat gggcaccacc acccatgccg
     7741 ctcgccgcga cggcaatgtc attattcatg tggcaaacgg tatcacgcat attggtccgg
     7801 cacggcaaca ggatggcgat tacagttatc tggcggatat tttgcaaacc gtgttgcctg
     7861 acgttgcctg gcataacaat attcgcgccg agctgtggcg caagctggca gtcaactgtg
     7921 tgattaatcc actgactgcc atctggaatt gcccgaacgg tgaattacgt catcatccgc
     7981 aagaaattat gcagatatgc gaggaagtcg cggcagtgat cgaacgcgaa ggacatcata
     8041 cttcagcaga agatttgcgt gattacgtga tgcaggtgat tgatgccaca gcggaaaata
     8101 tctcgtcgat gttgcaggat atccgcgcgc tgcgccacac cgaaatcgac tatatcaatg
     8161 gttttctctt acgccgcgcc cgcgcgcatg ggattaccgt accggaaaac acccgcctgt
     8221 ttgaaatggt aaaaagaaag gagagtgaat atgagcgcat cggcactggt ttgcctcgcc
     8281 cctggtagtg aagagactga agccgtcacc actatcgatc tgctggttcg cggcggtatc
     8341 aaagtcacca ctgccagcgt cgccagcgat ggcaacctgg cgattacctg ctcgcgcggc
     8401 gtgaagctgc tggcggatgc gccgctggtc gaagtagctg atggcgaata tgacgtgatc
     8461 gtgctgcctg gaggcattaa aggcgcagag tgttttcgcg atagcaccct gctggttgaa
     8521 accgttaaac agttccaccg ttccgggcgt atcgtcgcgg ctatttgcgc cgcaccagcc
     8581 accgtgctgg tcccgcacga tatcttcccg attggcaata tgaccggctt cccgacgctg
     8641 aaagacaaaa tacccgccga acaatggcag gacaagcgcg tcgtctggga tgcacgggta
     8701 aaattgctga ccagtcaggg gccgggtaca gctatcgact ttggtctgaa aattatcgac
     8761 ctgctggttg ggcgtgaaaa agcccatgaa gtggcatcac aactggtgat ggcggcaggg
     8821 atttataatt attacgagta gtggcggatg cggcaaacgt cgcatctgac cagatgcgac
     8881 gtaaaaacca caaattacgg gcgatatacc ttcacattgt taaagccctg ctcgcgcaga
     8941 tagagcgcct gcaggcggct catcaccccg cgctcacacc acaacagcca ggttttgttc
     9001 tggtcgagat cgccaaattt ggtgctcagt ttatagaacg gcagagaaac cacatcaatc
     9061 ccttcgactt tcagcggctt atcttcctgt tcatcgatag aacggatatc gaggatcacg
     9121 tcgttcgggc cgaagccatt gacggtttcc acttccacca cttcctgctc ggtctgctgg
     9181 gcgatttcgc ggatatcaac gttattcgct tcctcaacca ctttatcgag aatgctgaag
     9241 tcgaacttct cttcttccgc ttcaatcttc gatttaaccg ctttcaccgt cgggcttttg
     9301 gagatcacac cgcaatattc gggcatcgtg cgggcaaagt cttcagtgcc aatctggcga
     9361 gccaggttga tgatgtgctc tttgtcgtaa gaaatcagcg gacgcaggat cagcgtgtcg
     9421 gagacgttat caatcaggcg caggttagtc agcgtctggc tggacacctg gcccagtgct
     9481 tcgccagtga ccagcgcctg tacaccgtaa cgttcagcca ctttagacgc ggcacgcacc
     9541 atcatacgtt tgaggataac acccatctga ccgtcgtcga ttttctcgag aatttccccg
     9601 acgacaggtt cgaaattaat agcgacaaaa cgcacgcggt gggagctgcc aaagcggttc
     9661 cacagataat gcgccacctg acgcacgcca atttcatgcg ccgcgccgcc aaggttaaag
     9721 aagcagtaat gcacacgaca tccgcgacgc atcaacatat aactggaaac gccggagtca
     9781 aaaccaccgg aaatgagcga cagcacatct tcctgggtac caatcgggaa accgccaata
     9841 ccttcgtagc gacctttaat cagcaggaga cgatcgtctt ccacttccag atggacagtc
     9901 acatccggat tggtcagctt cacgcgcgcg gactcaatat gctgatttaa accgccgccg
     9961 acgtaacgtt ccacatcaat cgagctaaaa tcatgtttgc cacggcgctt cacgcgcacg
    10021 cagaaggttt tgccttccag ctgatcgcga tactgaacca acgctttctc gaaaatatcg
    10081 tgcatgtcgg taaacggcac gtcttcgact tcgagaatat ggtggatgcc cggaatacgg
    10141 gtcagggcgt cgcgaatagc cagacgctgg ttttcatctt ttgcgcgaac ttcgatgtta
    10201 tcccagtggc ggacgacagc gagcgtctca tcatagtgct ttaaaacgtt acgaatgttc
    10261 ccggtaagga tttttataaa gcgcaagcgc acagattggc ttttgatggt gatttccggg
    10321 aacaatttaa tgataaactt catggcggca atggttcgtt ggcaagcctt aagcgacttg
    10381 tatagggaaa aatacagcag cccacacctg cggctgcatc caggcgcgga agtataccac
    10441 taacatcgct ttgctgcgca catcacctta ccattgcgcg ttatttgcta tttgccctga
    10501 gtccgttacc atgacggggc gaaaaatatt gagagtcaga cattcattat gccgaagaaa
    10561 aatgaggcgc ccgccagctt tgaaaaggcg ctgagcgagc tggaacagat tgtaacccgt
    10621 ctggaaagtg gcgacctgcc gctggaagag gcgctgaacg agttcgaacg cggcgtgcaa
    10681 ctggcacgtc aggggcaggc caaattacaa caagccgaac agcgcgtgca aattctgctg
    10741 tctgacaatg aagacgcctc tctaacccct tttacaccgg acaatgagta atggactttc
    10801 cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc gctgagccgt tttatcgccc
    10861 cactgccctt tcagaacact cccgtggtcg aaaccatgca gtatggcgca ttattaggtg
    10921 gtaagcgcct gcgacctttc ctggtttatg ccaccggtca tatgttcggc gttagcacaa
    10981 acacgctgga cgcacccgct gccgccgttg agtgtatcca cgcttactca ttaattcatg
    11041 atgatttacc ggcgatggat gatgacgatc tgcgtcgcgg tttgccgacc tgccatgtga
    11101 agtttggcga agcgaacgcg attctcgctg gcgatgcttt acaaacgctg gcgttctcga
    11161 ttttaagcga tgctgatatg ccggaagtgt cggatcgcga cagaatttcg atgatttctg
    11221 aactggcgag cgccagcggt attgccggaa tgtgcggtgg tcaggcatta gatttagacg
    11281 cggaaggcaa acacgtacct ctggaggcgc ttgagcgtat tcatcgtcat aaaaccggcg
    11341 cattgattcg cgccgccgtt cgccttggtg cattaagcgc cggagataaa ggacgtcgtg
    11401 ctctgtctgt actcgacaag tacgcagaga gcatcggcct tgccttccag gttcaggatg
    11461 acatcctgga tgtggtggga gatactgcaa cgttgggaaa acgccagggt gccgaccagc
    11521 aacttggtaa aagtacctac cctgcacttc tgggtcttga gcaagcccgg aagaaagccc
    11581 gggatctgat cgacgatgcc cgtcagtcgc tgaaacaact ggctgaacag tcactcgata
    11641 cctcggcact ggaagcgcta gcggactaca tcatccagcg taataaataa acaataagta
    11701 ttaataggcc cctgatgagt tttgatattg ccaaataccc gaccctggca ctggtcgact
    11761 ccacccagga gttacgtctg ttgccgaaag agagtttacc gaaactctgc gacgaactgc
    11821 gccgctattt actcgacagc gtgagccgtt ccagcgggca cttcgcctcc gggctgggca
    11881 cggtcgaact gaccgtggcg ctgcactatg tctacaatac cccgtttgac cagttaatct
    11941 gggatgtggg gcatcaggct tatccgcata aaattttgac cgggcgccgc gacaaaatcg
    12001 gcaccatccg tcagaaaggt ggcctgcacc cgttcccgtg gcgcggcgaa agcgaatatg
    12061 acgtattaag cgtcgggcat tcatcaacct ccatcagtgc cggaattggg atcgcagttg
    12121 ctgccgagaa agaaggcaaa aatcgccgca ccgtctgtgt cattggcgat ggcgcgatta
    12181 ctgctggcat ggcgtttgaa gcgatgaatc acgcgggcga tatccgtcct gatatgctgg
    12241 tggttctcaa cgacaatgaa atgtcgattt ccgaaaatgt tggcgcgctc aacaaccatc
    12301 tggcgcagct gctttccggt aagctttact cttcactgcg cgaaggcgga aaaaaagttt
    12361 tctctggcgt gccacccatt aaagagctgc tcaaacgtac cgaagaacat attaaaggca
    12421 tggtagtgcc tggcacgttg tttgaagagc tgggctttaa ctacatcggc ccggtggacg
    12481 gtcacgatgt gctggggctt atcaccacgc ttaagaacat gcgcgacctg aaaggcccgc
    12541 agttcctgca tatcatgacc aaaaaaggtc gtggttatga accggcagaa aaagacccaa
    12601 tcaccttcca cgccgtgcct aaatttgatc cctccagcgg ttgtctgccg aaaagtagcg
    12661 gcggtttgcc aagctattca aaaatctttg gcgactggtt gtgcgaaacc gcagcgaaag
    12721 ataacaagct gatggcgatt actccggcga tgcgtgaagg ttccggcatg gtcgagtttt
    12781 cacgtaaatt cccggatcgc tacttcgacg tggcaattgc cgagcaacac gcagtgacct
    12841 ttgctgcggg tctggcgatt ggaggctaca aacccattgt tgcgatctac tccaccttcc
    12901 tgcaacgcgc ctatgatcag gtgctgcatg acgtggcgat tcaaaagctt ccggtcctgt
    12961 tcgccatcga ccgtgcgggc attgttggtg ctgacggtca aacccaccag ggcgcttttg
    13021 atctctctta cctgcgctgc ataccggaaa ttgtcattat gaccccgagc gatgaaaacg
    13081 aatgtcgcca gatgctctat accggctatc actataacga tggcccgtcc gcggtgcgct
    13141 acccgcgcgg caacgcagtt ggcgtggaac tgacgccact ggaaaaactg ccaattggca
    13201 aaggcattgt gaagcgtcgt ggtgagaaac tggcgatcct taactttggt acgctgatgc
    13261 cagaagcggc gaaagtcgct gaatcgctga acgctacgct ggtcgatatg cgtttcgtga
    13321 aaccgcttga tgaagcgtta attctggaaa tggccgccag ccatgaagcg ctggttaccg
    13381 tagaagaaaa cgccattatg ggtggtgcag gcagcggcgt gaacgaagtg ctgatggccc
    13441 atcgtaaacc agtacccgtg ctgaacattg gtctgccgga cttctttatt ccacaaggaa
    13501 ctcaggaaga aatgcgcgcc gaactcggcc tcgatgccac cggtatggaa gccaaaatca
    13561 aagcctggct ggcataatcc ctactctact cctgctatgc ttaagaaatt attcatggca
    13621 ggagtggcag catgcaatac aaccccttag gaaaaaccga ccttcgtgtt tcccgacttt
    13681 gcctcggctg tatgaccttt ggcgagccag atcgcggtaa tcacgcatgg acactgccgg
    13741 aagagagcag ccgtcccatc attaaacgcg cactggaagg cggcataaat ttctttgata
    13801 ccgccaatag ctattccgat ggcagcagcg aagagatcgt cggtcgcgca ctgcgggatt
    13861 tcgcccgtcg tgaagacgtg gtcgttgcga ccaaagtgtt ccatcgcgtt ggtgatttac
    13921 cggaaggatt atcccgtgca caaattttgc gctctatcga cgacagcctg ctccgtctcg
    13981 gcatggatta tgtcgatatc ctgcaaattc atcgctggga ttacaacacg ccgatcgaag
    14041 agacgctgga agccctgaac gacgtggtaa aagccgggaa agcacgttat atcggcgcgt
    14101 catccatgca cgcttcgcag tttgctcagg cactggaact acaaaaacag cacggctggg
    14161 cgcagtttgt cagtatgcag gatcactaca atctgattta tcgtgaagaa gagcgcgaga
    14221 tgctgccgct gtgttatcag gaaggcgtgg cggtgattcc atggagtccg ctggcgcggg
    14281 gccgtctgac gcgtccttgg ggagaaacca ccgcacggct ggtgtcggat gaagtgggga
    14341 aaaatctcta ccaagaaagc aatgaaaatg acgcacaaat tgcagaacgc ttaacgggcg
    14401 tcagtgaaga acttggtgca acgcgagcac aagttgcgct ggcctggttg ttgagtaaac
    14461 cgggcattgc cgcaccgatt atcggaactt ctcgggagga acagcttgat gaactgctaa
    14521 acgcggtgga tatcaccttg aagccggaac agatcgccga gctggaaacg ccgtataaac
    14581 cgcatccggt cgtaggattt aaataggtat gcaggcctga tgagacgtga caagcgtcac
    14641 atcaggcatc ggtgcacaac tacgacagaa tacccagcgg ccagtgatga ccgataaaat
    14701 acaggatgcc agcggagatc accccggcga caatatcgtc aatcatgatc cccatgccgc
    14761 catgtacatt gtgatcaaac cagcggatcg gccacggctt ccacatatcc agaatacgga
    14821 aaatcacaaa tccggcggta acccactgcc agtcattggt cggcagcgcc atgagcgtaa
    14881 tccacatacc aataaattcg tcccagacaa tactgccgtg atcgtgcacg cccatgtctt
    14941 tcgccgtttg atgacaaaga tagacgccga tacagatccc cagcattacc accagcgaat
    15001 agagctgcca gggcaaaaaa gtcatcagat accagaaggg aatcgccgcc agcgacccca
    15061 tcgtgccagg aacgatcggg cttaatccac ttccgaatcc gacagcaagc agatgccacg
    15121 gattactcat tttcaggcga cttttcgcga catctttatg gcgtggcaaa atggtcatat
    15181 cctttccagt ctaacgtgac aggttcgccg tcacgaataa aacaaagccc ttcgatatcg
    15241 gcggtcattt gcccgataca ggtaaacggt acgcccaggt gtccgagagc cacatccagc
    15301 gcgccacggt tcagttccgg caccgtgaaa cacaactcgt aatcttcacc accagagagc
    15361 gcccaacgca gcgcctgttc cggttcaaca tggcgagaaa gcgcatcaga aaacggcagc
    15421 aatgccaggt caatacgtgc gccgcagtcg ctggctttca cgatatgccc gagatcggaa
    15481 atcagaccgt cagagagatc gatagctgaa tttgccagat cgcgcagtgc ctgcccctgt
    15541 aaaatacgcg gcgacggacg gagatgacgt ttgatcaagt agtccgcatc tttagcatcg
    15601 gcaacctgca aacggttttg caaaatcgcc agcccggcag cgctatcgcc cggtgtaccg
    15661 gtcacataga tccagtcacc cggtttcgcc ccagagcgcg ttaaggctcg tcccatcgga
    15721 acaaagccgt ggatacccaa cgtcattgat aatggcccac gcgtggtatc gccgccaatg
    15781 agttgcatat cgtaataatt gagaagatca aacaagctgt cgctgaagga ctcaagccac
    15841 gcttcgtcta cgtccggtaa ggttaatgcc agcgtcagcc aggccggatc ggcccccatc
    15901 gctgccagat cgcttaggtt caccgccagt gctttataag ccagatcagc aggatcgata
    15961 tcagggagga agtggttgcc cgccaccagc gtatcagtgc tgatcgccag ggtctgtttc
    16021 tcggggatat tgagaagagc gcaatcgtcg ccgatgccca gttcgacatc aagacgagaa
    16081 cttcttacac ggtcaaaata acgggcaatc agggagaact cgccacatgc catacgttat
    16141 gcctcagcag gtaaaaaaga aaaggccggc gatcatggaa cggtcttccg tgaatctacc
    16201 ggcctggata ttactttttg ttagggcgaa tcacaggtgc tgctttatcg agtacgccgt
    16261 tgacgaactt atggctgtct tctgcgccga acgatttcgc cagttcgatc gcttcgttaa
    16321 tggccacttt gtatggcaca tcgctacgtt tagacagctc gtacagcgca atgcgcagta
    16381 ctgctttttc tacctggccc agttcttcca gcagacggga caggtacggc ttcatcagtc
    16441 cgtcgaggta tgcggtgtta gtcgccaccc cggccagcag ctcacggaag tacaggacgt
    16501 caacgtcttt tacatcctgt tcagccagga actggtattc aacatcagcg atgtcgttct
    16561 gggacaactg ccaggagtag agcgcctgga cggcacactc acgagcgcgg cgacgagcag
    16621 caggtttcac ggatttcccc ttactaattt caggccttga tggctttcaa tacattaatc
    16681 atttcaagcg cggtcagtgc agcttctgca cctttgttgc cagctttggt gccagcacgt
    16741 tcgatcgctt gttcaatgct ttcagtggtc agaaccccaa aagcaaccgg aatttcgctg
    16801 tcctgggcaa catgcgccag gccgttgctt gcaccaccag cgacatattc aaagtgggca
    16861 gtgccaccac gaataaccgt acccagcgca atcaccgcgt cgtatttacc ggttttagcc
    16921 agtgcacccg ccgccagcgg cagctcatag gcacctggca cccaaacaac ggtaatgttt
    16981 tcatctttta cctgaccgat acgtttcagt gcgtcaattg caccttccag caggctgtca
    17041 ttgataaagt tgttgaaacg cgcgatggtg atggcgacgc gagcgtccgg ggtagcaacg
    17101 ttagcttcaa taatgttcat tttcttcctt cgggttcgag tatggccccg caggggggcg
    17161 gattttagca taatatttcg tgcgctgctt ccctttcgag ccgggagatc atgcacccac
    17221 taaatgcagg caaacatccg ggcctacatg acgtatctct ttgaatttaa attggggagc
    17281 gtcggctaat ttctcaagcc ctggcagcgt gcataatcca cgggcgtcgt tgcctaatag
    17341 tttaggtgcg atatagacaa tcagctcatc gactaaaccc gcctgtagca atgcgccagc
    17401 gagcgttggc cccgcttcca cccagatgct gttaatctgc tgtttaccca gttgcatcat
    17461 tagtacaacc agatccaggt gacctttatg ctctggaatc agcaaggtac gcaccgtttc
    17521 cggccactca cgagaatctt cctgcgtacg cgcgaaccag gtttcgccgg gctgctgcac
    17581 aatgcgatgt tccggcgtca cgcgattttg gctatcaatc acaatacgta ccggctggcg
    17641 gagattttgt tgcgggtaga gtccctgagt ttgttcatcc agttcagacc aacgcactgt
    17701 taaggctgga tcatccgcca gcaccgtggc gctgctggtt aaaatggcat gactttgcgc
    17761 gcgcagtcgt tgtacgtcgc gcctcgcctg aggtgaagtg atccactggc tttcgccact
    17821 cgccatcgcc gtgcgaccat caagcgatgc gccaagtttt aactgaatat aaggaaagcc
    17881 ggtgcgcatc cgtttgagaa agcctttatt caattgctca gcttcactca tcatcagccc
    17941 gtggctgacg tcaatgccag cctgttgcag acggtaaagt ccacgtccag cgacctgcgg
    18001 attggggtct tgcattgcag caaccacgcg cgccacgccc gcagcaatta atgcatcaca
    18061 acacggtggc gtacgaccat gatggctaca gggttcgagt gtgacatacg ccgttgcgcc
    18121 tttggctttt tcgccggcca tgcgcaacgc gtgtacctcg gcatgcggtt cacccgcgcg
    18181 ttgatggtaa ccttcaccga caatttcgcc atctttgaca atgacgcacc cgacattcgg
    18241 gttgggatgc gtggtaaaac gtcctcgttg cgccagcttt agcgcccgcg ccatgtaata
    18301 ctcgtcctgc acggcttagt cctccaggcg cgcgatctct tcgccaaatt ctttgatatc
    18361 ttcgaaactg cggtagacag aggcaaaacg gatataggcg actttatcga gctttttcaa
    18421 ttgctccatc accagattgc caatcatctt gctcggcact tcgcgctcac cggtggcgcg
    18481 cagctgcgat tttatatgat tgattgccat ttcgacgtca tcggaactca ccggacgttt
    18541 ttccagcgcc cgcagcattc cgctacgtaa tttctcttca ttaaacggtt cacgcacgtc
    18601 gttgcttttt acaacacgcg gcataaccag ctccgccact tcaaaggtgg tgaaacgttc
    18661 attacacacc agacactgcc gacggcggcg tacggatgaa ccctcgccca cgagacgaga
    18721 gtcaattacc ttagtgtcca cggcgaaaca gaatgggcaa tgcatacggc gtcctgacca
    18781 ggtggttaac agaaatctat tttaccctga actgacgata caacaaaggc gcaacgcttt
    18841 tgagataaag gatcgatgct ttcactgctt ttaccctcta ccattaggct aattgcgacg
    18901 agaaagggac gacctatgaa tactaacgtt tttcgactgc tcctgctggg aagcctgttc
    18961 agccttagcg cctgtgtgca gcaaagtgaa gtgcgacaga tgaaacacag cgtcagcacg
    19021 ctgaaccagg agatgacgca actcaataaa gaaacggtca aaatcactca gcaaaacaag
    19081 ctgaatgcaa aatccagcag tggggtttac cttctgcctg gagcaaaaac accagcaaga
    19141 ctggaaagcc agatcggtac tttacgtatg tcgctggtga atattacgcc tgacacagat
    19201 ggcactaccc tgacgctacg tattcagggc gaatccaatg accctttacc cgcattcagc
    19261 ggaaccgttg aatatgggca aattcaggga acaatagaca actttcagga aatcaatgtg
    19321 cagaatcaat tgattaacgc acctgccagc gtcctcgccc ccagcgatgt tgatattccg
    19381 ttacagttaa agggtatgtc tgtggatcag ttaggctttg tacgtatcca cgacattcaa
    19441 cctgttatgc agtaaacgta ttgcggggcg atattgtgcg tcccgcaaca tctttctccg
    19501 tcattttgtt actccgctta catcacctgg attgatagta aaagtttgca acaagggtga
    19561 aagtcagtac aatccccgcc cgaatgtgtg taaacgtgaa cgcaatcgat tacgtaaatg
    19621 atagaactgt gaaacgaaac atatttttgt gagcaatgat ttttataata ggctcctctg
    19681 tatacgaaat atttagaaac gcaatttgcg cctttttcac tcccgcaagg gattttcaaa
    19741 cagtggcata catatgaaaa aaacattact ggcagccggt gcggtactgg cgctctcttc
    19801 gtcttttact gtcaacgcag ctgaaaacga caaaccgcag tatctttccg actggtggca
    19861 ccagagcgtt aacgttgtcg gaagctatca cacccgtttc ggaccgcagc tccgcaacga
    19921 tacctacctt gagtacgaag cattcgctaa aaaagactgg ttcgacttct atggttatgc
    19981 ggatgcgccg gtattcttcg gcggtaactc cgatgcaaaa ggtatctgga accacggttc
    20041 tccgctgttt atggaaatcg aaccgcgttt ctccatcgac aagctgacca atactgacct
    20101 tagctttggt ccgttcaaag agtggtactt cgcgaacaac tacatttacg acatgggtcg
    20161 taataaagat ggccgccaga gcacctggta catgggtctg ggtaccgaca tcgatactgg
    20221 tctgccgatg agcctgtcca tgaacgtcta tgcgaaatac cagtggcaga actacggcgc
    20281 agcgaacgaa aacgagtggg acggttaccg tttcaaagtg aagtactttg tgccgattac
    20341 cgatctgtgg ggcggccagc tgagctacat cggcttcacc aacttcgact ggggttccga
    20401 cctgggcgat gacagcggtt atgcgaacaa cggtattaaa acacgtacca ataactctat
    20461 tgcgtccagc catattctgg cgctgaatta cgatcactgg cactactctg tcgttgcccg
    20521 ttactggcat aacggtggtc agtggaatga cgatgcagaa ctgaacttcg gcaacggcaa
    20581 cttcaacgtt cgctctaccg gctggggtgg ttatctggta gtaggttaca acttctaatt
    20641 gtgagaatgc cggatttcat atccggcatt ttttatcact tcttcttgtt catcatcgct
    20701 ttcaaatcag cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct
    20761 gcgataacgg tcgtaccata ctgatccgct tcggtgtatt tcgaatgctc atgatcgtgg
    20821 caatagagac aaagtaattc ccagttactg ccatcttccg ggttattagt atggtcgtga
    20881 tcaatgtggt gaacggtaag ttcacgcagg ttggaataaa caaactcgcg ggaacagcga
    20941 ccgcagaccc acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgcgca
    21001 tagttttttg ggatgatagc catgaaaaag ttacctggat gaaatgtagg gttacgcgca
    21061 gtttcccaag atgcggggat tttcgcaatc accttacatc aaaaaaaatc ccgatcttct
    21121 gaccgggatt ttcaacatca gctgaaactt gattaaggca gaattgacgg ctgatccgcc
    21181 ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag tttcagagcc
    21241 agcgccgatg ccacatagat ggaagatgca gtaccgatgg aaacaccgat aagcatggtc
    21301 agcgagaagc cttccagtac cggaccaccg aagaggtaca gcatcaggat aaccatcaag
    21361 gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac gttaaagatt
    21421 tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac gaatacggtc cgataccacg
    21481 atactgtcgt taagcgagta accgataact gacatcaacg atgccacaat ggtcaggtca
    21541 atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg tgccagcgca
    21601 ataacaaccc ctgccgccag tcgccactca aagcggaaac ctacgtacac gaggatagac
    21661 agcagcgccg ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc cacgctcgga
    21721 ccgacgaact caatacgctt cactgccgca ttctgattgg tggattcgtt aatcaccttc
    21781 aggacctggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg catacggacc
    21841 atgatgtcgt ggctgctacc aaagttttgc agcatcggct cttcaaaacc ggctttttgc
    21901 aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc aataaccgta
    21961 ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac gatagcggcg
    22021 attaacagca gaccagagat gccaaaagcc cagtagtccc agcgcataaa gtcatagact
    22081 ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca ctcctcagat
    22141 tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac gcgtaccgac
    22201 aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga accctttaat
    22261 tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga tgttcgcatc
    22321 gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa cagtacgtcc
    22381 gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga ccgctaccgc
    22441 aagggttaag acgatacccg cgatacctgg catactcagc gttgcgcctg gcaacagcga
    22501 cataataccg acaatcaaga tcaagttggc aatcagagca ctggtcgcaa tcagaccaaa
    22561 cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa ggcaagcctc
    22621 cagcccctgc tcaatgttct gcatacccag ggttgggcca atggtacgtt cttcaacaat
    22681 ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gaaagctgac gcgcttcgct
    22741 tgggttattg atgccagtaa tacggaagct gttacccaga cgagactgga tgttggcgat
    22801 attaatcacc tcttcctgtt tcaccagaac cgcacgaccg tttgcatctt tcttaccgct
    22861 gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct tggtgaagtt
    22921 agacatgatg ttaccacccg cgctatcgag cgagatgtta acctgcggct ggttgtattc
    22981 gtcctggctg gaagtggagt cggtgatatg gtcaccggtc agaattacgc gtttgtacag
    23041 cacaaccggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta cgcgaccgga
    23101 tgccgcagcg gcctgatcaa cgttggtgtt taccagacgg aattccagcg ttgccgtcgc
    23161 acccagaatc tctttcgcac gcgctgtgtc ctgaatacct ggcagttcaa caacgatacg
    23221 gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta cacggttacg
    23281 caggatatta atgttctgct gcaccgcata ttcacgcgct tcactcaggc gagcatcgct
    23341 cattaccgca cgcagctggt tgctgccctg gctgctaatc accaggtccg gatggcgctt
    23401 gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtga tgctcaggcc
    23461 gtagttgttt tctttacgaa cggtggtata cgggatgccc ttttcgcgca ggtcgctgcg
    23521 caggctatcg atgttttgtt cctggagttt gccaagcgcg gtgtccatat ccacttccat
    23581 caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag cgtgaatagc
    23641 tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt tgtcacccat
    23701 aacgcccatt aatgcttcac gagcgcgcag ctgggtgtca gtggagtcga agcgcgcaag
    23761 aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt gtaacgtttt
    23821 ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag tgatctgaac
    23881 agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga caatcaccac
    23941 gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc ccttagggaa
    24001 aaattttaat tacagcgcct tcatggtgcc tttcggcaga acggcagcta cgaagtcacg
    24061 tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt tttccgctac
    24121 tttggttacg cgaccgacca ggccaccgtt cgtcagcact tcatcacctt tagcgatgga
    24181 gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga tcatgaaata
    24241 gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac cttgcgccgg
    24301 tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc cctcattatt
    24361 aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa aaatcagtta
    24421 cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc aaacgctggt
    24481 agtaacgaag gttatgaatg gtattgagtc gcgcgcctaa tatttcgttg caacggtcga
    24541 gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac tcaggatcga
    24601 gtgggccagt atcgctctta tacttcgcat tgcggatttt caccacgcca tcggtcacga
    24661 acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata tcgataccgc
    24721 gacgcacgcc ttcaaccagg tcttctggtt taccaacgcc catcaggtaa cgcggtttgt
    24781 ctgccggaat ttgcgggcat acatgctcca gaatgcggtg catatctgct ttcggctcac
    24841 ccacagccag accgccgaca gcgtagccat caaaaccgat atctaccaga cctttaacag
    24901 aaatatcacg taaatcttcg taaacgctgc cctgaatgat accaaacagt gcatttttgt
    24961 ttccgagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac atctccatgg
    25021 agcgttttgc gtaatcccag tcagcaggat acggcgtaca ctcatcaaag atcatgacga
    25081 tatccgaacc aagatcgtac tgaatctcca tcgacttttc cgggtcgagg aaaatcgggt
    25141 cgccgttgat cgggttacgg aagtgcacgc cctgttcggt gattttacga atatcaccaa
    25201 ggctgaagac ctggaagccg ccggagtcgg taagaatcgg ccctttccac tgcataaaat
    25261 cgtgtaaatc gccatgcagt ttcatgattt cctggcccgg gcgcagccac aggtggaagg
    25321 tgttaccgag aataatttgt gcgccagtgg cttcaacttc ttccggcgtc atccctttta
    25381 cggtgccgta agtgccaaca ggcataaaac aaggcgtttc cactacgcca cgatcaaaga
    25441 ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc attttttctc
    25501 cactacgtca gaaaaacagt ccaacgttta aaccagcgcc gcggaattac tccccaacgc
    25561 gctcattaat tgcctgcgga ttgtacgtga taaacatcgc atcaccgtaa ctaaaaaagc
    25621 gatatttctc ttctaccgcc gctttatagg cattcatggt gtgttgataa ccagcaaatg
    25681 ccgaaaccag cataatcagc gtcgattccg gaaggtggaa gttcgtcacc agcgcatcga
    25741 ccactttgta ctggaaaccc ggatagataa agatttgggt atcgtcaaag aacggttcaa
    25801 tgagatcgtt tttcgctgcc tgagccgcac tttccagcga acgtactgag gtggtaccaa
    25861 ccgcaatcac ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg
    25921 gcacttcagc gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct
    25981 ggaaggtgcc cgcaccaacg tgcaacgtca caaacgccat ctccacgcct ttggcgcgta
    26041 atttttccag caaaggttcg tcaaagtgca gtccggcggt cggtgctgct accgcgccgg
    26101 gtttttcgct ataaaccgtt tgataaagtt cgcggtctgc atcttcgtcc ggacggtcga
    26161 tgtacggcgg cagcggcata tggccgatgc tgttgagaat atccagcacc gaacgttgat
    26221 cattaaattc gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg ttaatacgtt
    26281 cgtcatcgcc cagcagcagt tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg
    26341 caagaatgcg tttgtcgtcg agcatccgct caaccagcac ttcaatcttg ccgccgctgg
    26401 ctttacgccc aaacagacgc gccgggatca cgcgggtatt attaaaaacc agaagatcgc
    26461 cggggttgag cttatcgagt aaatcggtga aagtaccgtg cgtcagcgcg cccgtcggcc
    26521 cgtccagcga cagtaaacga cagctactgc gttcaggcat gggatagtgg gcaatcaggg
    26581 attcgggcaa ttcaaaagag aaatcggtaa cgcgcatgac actgactcag actaaaataa
    26641 gaggcgggta gtctagtgcc ggggcgctct ccctgcaacc tttacccccc tttggataga
    26701 atataatcga tgaatttttt agctcacctg catttagccc atctcgcgga aagctcgctt
    26761 tccggcaatt tactggctga tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac
    26821 gtcgtggctg gcattcatat gcatcggcga atcgacgtat taaccgacaa tctgccagaa
    26881 gtccgcgaag cgcgggagtg gtttcgtaat gaaacgcgcc gtgtcgcgcc tattacgctg
    26941 gatgtgatgt gggatcactt tctgtcccgc cactggtcgc agctgtcgcc cgattttccg
    27001 ctacaggaat ttacctgtta tgcccgggag caagtgatga ctattcttcc agactcaccg
    27061 ccacgcttta tcaatctgaa caactactta tggtcagagc agtggttggt gcgctatcgc
    27121 gatatggatt tcatccagag cgtgttaaac ggcatggcaa gccgtcgccc acgtctggat
    27181 gccctgcgta actcctggta cgatttagac gcccattacg atgctctcga aacctgcttc
    27241 tggcagtttt atccgcggat gatggagcag gcgtcacgca aggcgttata agacgttaat
    27301 tcatccacac cgtagccgaa atggcaggca aagccagaat cccgtctgtc agttgcccgt
    27361 gaccttcttt gcgttgccat tgcgcgacat taagcaacgg cgacgcgggt agcaccactt
    27421 cacaggcttc gccacggttg attgccacca gtacacgctg ctgatacagc acgcggacaa
    27481 ataccaccac gttatcttcc gcatacagta cctgacagcc gccacgacgc agcgcctgac
    27541 ttttcttacg cagcgcaatc attcgctggt acagcgcgaa taacgccgta tcctgctttt
    27601 ccacctgcca ggggaacggt ttacggcaaa acggatcgtt tttaccatcc agtcccactt
    27661 catcaccgta ataaatgcac ggtacaccag gccaggtgaa caaccagacc accgccagcg
    27721 gcaggcgcgc aatatcccga ccgagcagcg ttttaaatcg cgcagtatcg tggctatcga
    27781 gctgattaaa catacgcagt tgctgttgat gagaaagtcc ggcgcggtaa ttatccatcc
    27841 aggccataca ggtttgggcg tcaatttgct gcggatcgta agagatgtcg gtattggcaa
    27901 gaaatcccca caacgggaat gtgaagccgc gatagttcat ggcggcatct tccacatcgg
    27961 cctgtaacca ttgccgtgca tcgccaaaat gctctccaac gatatacgct tccggctggg
    28021 tttctttcgc cgcttcggtg atcccggcta cgtgctgcaa attattgcgc gccccacccg
    28081 cttcccccag catatgcacc acatccagcc gccagccgtc catattccac ggcgctttca
    28141 gccagtggcg gacgatactg tcttccccac gataaatttc attcaccaga ctttccgact
    28201 gataatccag tttcggcaag ctggcatagc ccagccagtc gagcgccgtg ccatcgtcac
    28261 taaacgagta ccagtcgcgc cagggcgatt cggggttgtg acaagcacca cccgtcccgc
    28321 gattgtgtct gtcaaaccag gcatgggaat cgccactgtg gttaaacacg ccgtccagca
    28381 ccagccgcat tcccagctgc tgcgtattgt gtcgcaaacg caacaacgcc ccatcaccgc
    28441 caaactgcgg atcgacatgg cgataatcct cggtatcgta tttatgtacg ctgggagctt
    28501 taaacaccgg attgagatac agcgccgtca cgccaagttt tttcagatac ggcagttttt
    28561 cgcttatccc gtctagatcg ccgccataga acgttgatcc gcccgcctgc gccgtgaccg
    28621 gttcatccca gtcacgcaag atgatctctt gtccggctgc gtgatggtaa tagacatgat
    28681 cctgttcagc ttcacgagga agactacggg cgaaacgatc agggaagatc tgataaaaaa
    28741 tctgatccgc agcccattgt gggccgatat ccggtacatc gacggcaaac tgctccagtc
    28801 gtgccggcgg cattcggctg aagccctgcg gtgtaaacca acgctggcga tcgtgccaca
    28861 gtaatttgaa actataacgc cgccgaggta gtccgccgga gagatcgatc gccgcccgcc
    28921 atgcggtaac accaggctgc ggctgactgc gctgcttatg catcggtact gacgtttctt
    28981 cgttgtcgtg ttctgtacgc agcataatgc gctgcggtgg gtcttcgccc gtcagccaca
    29041 gtgtaatgag cagttgatct ttgctttgtt taacaaatgg gggcaccggc aggtgccatg
    29101 catttaacat cataattccc ctgtgatgaa aatctgatca ccttgccata gcctaatgca
    29161 ggatcactca tcatctgaag gatatttggg ggaggaggac gggggcggat aactttcgga
    29221 atagccggat caggatttcc tcctgaaccg gcttacagaa cgcaaggatt agtggttttc
    29281 agccagctga cgatcgtggc ggcgcttaaa catccagcca atcaacagca caacaatcca
    29341 cgcgaaaccg acatacagcg agatacgagt atccgggtga taaccaatca acccgataat
    29401 aaagagcagg aaaatcaaac cgccgatggt ggttgctacc ccacccggta ctttaaattt
    29461 cagcgactta acttcttctg gcggtaaacg gcgacggaag gcgatttgcg acagcaggat
    29521 cataatccac acccacaccg tggcgaaggt tgccagcgaa gcgatcacca ggaagacgtt
    29581 ttccggcatg atgtagttca gatacacagc aaacagcagc gcggtagtca tcaccagcac
    29641 cgtaacccac ggaataccgc gacgcgaggt tttgctgaaa atttttggtg cgctgccctg
    29701 ctctgccata ccgtggagca tacggcctac gccaaatacg tcactgttaa ttgccgacag
    29761 cgaagcggtc agcacaacaa agttaagaat gctggcggca aaggtaatgc ccatatgctg
    29821 gaacgtcagt acgaacgggc taccggcagt gccaacctga ttccacgggt agatagacat
    29881 aatgacgaac agcgtaccga cgtagaacac cagaatacgc atcggcacgg agttaatcgc
    29941 acgcggaatc gatttctcag gatctttcgc ttcaccggcg gtaatcccga taatttcgat
    30001 cccaccgtaa gcaaacatca ccatttgcaa cgacatcacc atcccaagcc agccgttact
    30061 gaagaagccg ccgttgctcc acaggttatg aataccggtc agttgcccgc cgttgccaat
    30121 cccccagatg atgatgccga aaccggcgac aatcatgatg atgatggtgg cgactttaaa
    30181 gaacgagaac cagaactcca gctcaccgaa taccttcacg ctcatcaggt ttacggcgca
    30241 aatgatcagc accacgctca gtacccaaat ccagtgcggc accgtcggga accagacacc
    30301 catatagata ccaaaagcgg tcacatcggc gatggcgaca ataaggattt caaagcagta
    30361 ggtccagccg gtaatgtaac cagccagcgg gccgaggttt tcctgcgcat aacgcgagaa
    30421 agagctggcg gccgggttat gtaccgacat ttcccccagc gcgcgcataa tgatatacgc
    30481 cgcgacacca ccgataatat aggccaacaa cacgctcgga ccggccattt tgatggcgtc
    30541 tgccgaaccg taaaacaacc cggtgccaat tgctgaaccc agtgccataa agcgtatgtg
    30601 gcgggtactt agcccacgct ttagcttgtt cttactttcc atcaattcct aacccatcaa
    30661 cacaataaaa aaccacgggg catcgcagcc ccgtggttaa aacaaatgtt cagtgattta
    30721 gtgagcgctg gaggtcacct gacgacctgc cgcgcgatcc cagataatgg ccagaaccac
    30781 catcaccact gttggcatta accacgccag accttgttcg gccagcggta aacgctgcgc
    30841 ccaggacggt aagatatcgc tgaatgcaga tgctttgatc ccgtcgagaa taccaaaaag
    30901 caggctgata aacatcggcg gagcaatcac gcgggacgaa ttatgccacc atgagcgtgt
    30961 aaaacttaat acaaccagtg cgatacacgg cggataaatg gcggtcagca ccggtacaga
    31021 gatctgaatc agctgactca agccgaggtt agacaccacc atcgagaagc cgccgaggat
    31081 aaacaccagc gtacgataag agagcggtac gtactgggcg aagaattctg cacaagcaca
    31141 ggtcaggcca accgccgtga ccaggcaggc gataaagatt aacgccgcca gcaggaagct
    31201 accgccgccg ccaaaggtat gctgaacgta agcatgcagg atcgccgcac cgtttgcaga
    31261 ctgatcgacc agcgacgcgc tgtctgaacc cagacggaac agcgccaggt acagcagagt
    31321 cagaccaaca cccgccatca ggccagccca gacggtataa cgggtcagca gacgcgcttc
    31381 ggtaacgcca cgagaacgcg ccgcgttaac aataacgata ccaaacacca ttgcgcccag
    31441 cgtatccatg gtcagatagc cgttgacgaa gccgttagaa aacgcagcgt tttgataagc
    31501 ctcagtagcc gtgctgatag aacccgccgg ccagataatt gcggcaacag acaggatgac
    31561 cagcgcgata attttcagcg gcgcaaggaa gttgcccacg gtatccagca gcttgcccgg
    31621 atagagcgaa accagaataa cgatagcgaa atagaccagg ctgtaaataa acagcggcag
    31681 tgcggaatca cccgtcagcg gcgcaatccc cacttcaaaa gaaacggtag ctgtacgcgg
    31741 cgtagcgaac agcggcccca ccgccaggta acacaccgtt gccagcagta cgccagcaac
    31801 tttaccgatt ggcgtgctga ggctgtcaac accgccgcca acttttgcca gcgccaccac
    31861 cgttaatacc ggcaggccaa cggcagtaat gaggaagccg aatgccgcag tccagacgtg
    31921 ttcgcctgcc tgcaagccga ccattggagg gaaaataatg ttacctgcgc cgacgaacaa
    31981 cgcaaatgtc ataaagccca gagcgatgat atcgcgcgat cttaattgat gggtcataaa
    32041 tcatactgcc tgtggatgtg gtgttgtaac gttaaatttt tgtgccaatc cctggggatg
    32101 atacagcggt atatttaaac aattttagcg ggaaatactc ccaaccaggc gcggcgaaga
    32161 atagtttttc gatttaccgc gttaatgcag tcagtatgac agcacctgaa gcgcaattta
    32221 aacgcttata acgttttaaa gcaagaaggg atctaaaaac cagaggtata tagcgataag
    32281 aaatcaccaa ccagtataaa acactatttc aatgaaaaag acatggctaa tcatgcgaac
    32341 aaaaaatcac caaccgttgt aaaatctaac gcccgtattt cgactgcaaa gaaaataagc
    32401 cagcatcggc tggcttatgg caataaaaga tgacaaaggc gaattaatcg ctgtttttgg
    32461 caattaaacg ttccgggata acaaaactga aacgtgtaac ttttcctact gtactctcaa
    32521 tattcaggcg actttcgtga tgattcaccg catgtttcac gatcgctaac cctaatccgc
    32581 taccaccggt ttgccgggaa cgcgctttat caacgcgata aaaacgctcg gtcaggcgcg
    32641 gaatatgctc cggtgcaatg cccggtccgt tatcttcaac actaaattcg gcaccgtgcg
    32701 gcactcgctg ccagcgtacg gtgatatgcg tgccttccgg cgtatgattc acggcgttat
    32761 agaccaggtt cgaaatcgca ctgcgtagct gatcttcgtt gcctgacacc ttgaggccgt
    32821 tatctatctc aaaggtaaat gtctgttttt tctgactcag agtctgagcc tcgcgctcaa
    32881 cgacgcgcag catcatcggc acatcaacct tttcattgag caaatgcgtc ggtgcggctt
    32941 ctattttcga cagcgtcagc aattgcttca ccaacccttc catccgctgg gtctgctcgc
    33001 gcatggtgtg caacgctttt tcgcgtaccg cgccttccag cggctgctca tccatcatct
    33061 ccaggtaacc ctgtaacacg gtcaatggcg tacgtaactc atggctcacg ttggcaaaaa
    33121 agttacgccg cgccccttcc agttgatgca tttgcgtgac atcacgcgcc accatcagca
    33181 actgtttgtg ggtataaggc ataacgcgaa tttccagatg ccgcccggtg ttgagcacca
    33241 gattaagcgg gcgagaaaaa tcacgcgttt tcagatattg cgtaaactcc gggtaacgca
    33301 gcaggttaag gatgttctgc ccgttatctt ccggccagcg caaaccaaga atctgttgcg
    33361 ccagaccgtt acaccagaaa ataccgccct cttccgtggt cagcactact gcgtcgggca
    33421 gcgactccgc gccactacga aagcgtttaa tcagattgcc cagttcgcgg cggcgttttt
    33481 tatttcgcag ctgcatctgg tgtaagccgt atagtagcgg ttcccagcta ccacgccccg
    33541 gtggcggggt catactgcga tccacccaca gccaccatga aaggcgcaat aaattccaga
    33601 aatgccagat aagcagtcct gttaccgatg ccagcaaaaa ccagggcagg taaccaaaaa
    33661 atgcacccag gatgaaagcc gggaggcagc aaagtagcag ctccagcacc agccttttcc
    33721 acgacagccg ttccagcacg taagatactc cagttaagaa atcataagcc ctgctctgcg
    33781 tccgatgagc aaggcgttaa aagcgggttg aaaaacgata tcctgtaccg cgcacggtct
    33841 gcaccatgcg gtcatgcccg ccgggctcca gtgctttacg caggcgacga atgtggacat
    33901 cgaccgtgcg gtcttccaca tacacgttag ttccccagac gtggtttaac agctgctcgc
    33961 ggctgtacac gcgctcagga tgcgtcataa aaaagtgcag cagtttaaat tctgtcggcc
    34021 ccatctccag tggctcttcg cccgccatca cccggtgaga tgtcgggtcg aggcttagcc
    34081 cctgcatctc gatcacctct tccaccgcca ttggcgaaat acggcgcatt accgctttga
    34141 ttcgcgccac cagctccttc ggcgaaaacg gcttggtgat atagtcatcc gcgccggttt
    34201 caaggccgcg cacgcgatct tcttcttccc ctctggcggt caacatcacc actggaatat
    34261 cccgggtcat cgactcgcgc ttgaggtgtt tgatgaactg gataccggag ccgccaggta
    34321 acatccagtc aaggagaatt aaatccggcc agggttcatt cagttgattc acagcactgt
    34381 cataatcttc cgcttcgacc ggctgaaagc cattttgttc gagcacgaag cagaccattt
    34441 cgcgaattgg agcttcatct tctacgacca gaatacgtct cgccatgatt tgccctgttg
    34501 taataaatag gttgcgatca ttaatgcgac gtcattatgc gtcagattta tgacagattt
    34561 atgaaaagct cgtcgcacat atcttcaggt tattgatttc cgtggcgcag aaaaaagcaa
    34621 atggcacatc tgtttgggta taatcgcgcc catgcttttt cgccagggaa ccgttatgcg
    34681 catccttcac acctcagact ggcatctcgg ccagaacttc tacagtaaaa gccgcgaagc
    34741 cgaacatcag gcttttcttg actggctgct ggagacagca caagcccatc aggtggatgc
    34801 gattattgtt gccggtgatg ttttcgatac cggctcgccg cccagttacg cccgcacgtt
    34861 atacaaccgt tttgttgtca atttacagca aactggctgt catctggtgg tactggcagg
    34921 aaaccatgac tcggtcgcca cgctgaatga atcgcgcgat atcatggcgt tcctcaatac
    34981 aaccgtggtc gccagcgccg gacatgcgcc gcaaatcttg cctcgtcgcg acgggacacc
    35041 aggcgcagta ctgtgcccca ttccgttttt acgcccacgt gacattatta ccagccaagc
    35101 ggggcttaac ggtattgaaa aacagcagca tttgctggcg gctattactg attattatca
    35161 acaacactat gccgatgcct gcaaactgcg cggcgatcag cctctgccca tcatcgccac
    35221 gggacattta acgaccgtgg gtgccagtaa aagtgatgcc gtgcgtgaca tctatattgg
    35281 cacgctggac gcgtttccgg cacaaaactt tccaccagcc gactacatcg cgctcgggca
    35341 tattcaccgc gcacagatta ttggcggcat ggaacatgtt cgatattgcg gttcccccat
    35401 tccactgagt tttgatgaat gcggtaagag taaatatgtc catcttgtga cattttcaaa
    35461 cggcaaatta gagagcgtgg aaaacctgaa cgtaccggta acgcaaccca tggcggtgct
    35521 gaaaggcgat ctggcgtcga ttaccgaaca gctggaacag tggcgcgatg tatcacagga
    35581 gccacctgtc tggctggata tcgaaatcac tactgatgag tatctgcatg atattcagcg
    35641 caaaatccag gcattaaccg aatcattgcc tgtcgaagta ttgctggtac gccggagtcg
    35701 tgaacagcgc gagcgtgtgt tagccagcca acagcgtgaa accctcagcg aactcagcgt
    35761 cgaagaggtg ttcaatcgcc gtctggcact ggaagaactg gaggaatcgc agcagcaacg
    35821 tctgcagcat cttttcgcca cgacgttgca tagcctcgcc ggagaacacg aagcatgaaa
    35881 attctcagcc tgcgcctgaa aaacctgaac tcattaaaag gcgaatggaa gattgatttc
    35941 acccgcgagc cgttcgccag caacgggctg tttgccatta ccggcccaac cggtgcgggg
    36001 aaaaccaccc tgctggacgc catttgtctg gcgctgtatc acgaaactcc gcgcctttct
    36061 aacgtttcac aatcgcaaaa tgatctcatg acccgcgata ccgccgaatg tctggcggag
    36121 gtggagtttg aagtgaaagg tgaagcgtat cgcgcgttct ggagccagaa tcgggcgcgt
    36181 aaccagcccg acggtaattt gcaggtgcca cgcgtagagc tggcgcgctg cgccgatggc
    36241 aaaattctcg ccgacaaagt gaaagataag ctggaactga cggcgacgtt aaccggactg
    36301 gattacgggc gcttcacccg ttcgatgctg ctttcgcagg ggcaatttgc tgccttcctg
    36361 aatgccaaac ccaaagaacg cgcggaattg ctcgaggagt taaccggcac tgaaatctac
    36421 ggacaaatct cgtcgatggt gtttgagcag cacaaatcgg cccgcactga gctagagaag
    36481 ctgcaagcgc aggccagcgg cgtcgcgttg ctcacgccgg aacaagtgca atcgctgaca
    36541 gcgagtttgc aggtacttac tgacgaagaa aaacagttac ttaccgcgca gcagcaagaa
    36601 caacaatcac taaactggtt aacgcgtctg gacgaattgc agcaagaagc cagccgccgt
    36661 cagcaggcct tgcaacaggc gttagccgaa gaagaaaaag cgcaacctca actggcggcg
    36721 ctcagtctgg cacaaccggc acgaaatctt cgtccgcact gggaacgcat cgcagaacac
    36781 agcgcggcgc tggcgcatac tcgccagcag attaaagaag taaatactcg cttacagagc
    36841 acaatggcgc ttcgggcgag cattcgccac cacgcggcga agcagtcagc agaattacag
    36901 cagcagcaac aaagcctgaa tgcctggtta caggaacacg accgcttccg tcagtggaac
    36961 aacgaactgg cgggttggcg tgcgcagttc tcccaacaaa ccagcgttcg cgagcatctg
    37021 cggcaatggc agcaacagtt aacccatgct gagcaaaaac ttaatgcgct tacggcgatc
    37081 acgttgacgt taaccgccga tgaagttgct accgccctgg cgcaacatgc tgagcaacgc
    37141 acgctgcgtc agcgcctggt cgcgctgcat ggacagattg ttccccaaca aaaacgcctg
    37201 gcgcaattac aggtcgctat ccagaatgtc acgcaagaac agacgcaacg taacgccgca
    37261 cttaacgaaa tgcgccagcg ttataaagaa aagacgcagc aacttgccga tgtgaaaact
    37321 atttgcgagc aggaagcgcg catcaaaacg ctggaagctc aacgtgcaca gttacaggcg
    37381 ggtcagcctt gtccactttg tggttccacc agccacccgg cggtcgaggc gtatcaggcg
    37441 ctggagcctg gcgttaatca gtctcgatta ctggcgctgg aaaacgaagt taaaaagctc
    37501 ggtgaagaag gtgcgacgct acgtgggcaa ctggacgcct taacaaagca acttcagcgc
    37561 gatgaaaacg aagcgcaaag cctccgacaa gatgagcaag cacttactca acaatggcaa
    37621 gccgtcacgg ccagcctcaa tatcaccttg cagccacagg acgatattca accgtggctg
    37681 gatgcacaag atgagcacga acgccagctg cggttactca gccaacgaca tgaattacaa
    37741 gggcagattg ccgcgcataa tcagcaaatt atccagtatc aacagcaaat tgaacaacgc
    37801 cagcagcagc tcttaacggc attggcgggt tatgcactga cattgccaca ggaagatgaa
    37861 gaagagagct ggttggcgac gcgtcagcaa gaagcgcaga gctggcagca tcgccagaac
    37921 gaattaaccg cgctgcaaaa ccgtatgcag cagctgacgc cgattctgga aacgttgccg
    37981 caaagtgatg aactcccgca cagcgaagaa actgtggtgc tggaaaactg gcggcaggtt
    38041 catgaacaat gtctcgcatt acacagccag cagcagacgt tacagcaaca ggacgttctg
    38101 gcggcgcaaa gtctgcaaaa agcccaggcg cagtttgaca ccgcgctaca ggccagcgtc
    38161 tttgacgatc agcaggcgtt ccttgcggcg ctaatggatg aacaaacact aacgcaactg
    38221 gaacagctca agcagaatct ggaaaaccag cgccgtcagg cgcaaacgct ggtcactcag
    38281 acagcagaaa cgctggcaca gcatcagcaa caccgacctg acgggttggc tctcactgtg
    38341 acgatggagc agattcagca agagttagcg caaactcacc aaaagttgcg tgaaaacacc
    38401 accagtcagg gcgagattcg ccagcagctg aagcaggatg cagacaaccg tcagcaacaa
    38461 caaaccttaa tgcagcaaat tgctcaaatg acgcagcagg ttgaggactg gggatatctg
    38521 aattcgctaa taggttccaa agagggcgat aaattccgca agtttgccca ggggctgacg
    38581 ctggataatt tagtccatct cgctaatcag caacttaccc ggctgcacgg gcgctatctg
    38641 ttacagcgca aagccagcga ggcgctggaa gtcgaggttg ttgatacctg gcaggcagat
    38701 gcggtacgcg atacccgtac cctttccggc ggcgaaagtt tcctcgtcag tctggcgctg
    38761 gcgctggcgc tttcggatct ggtcagccat aaaacgcgta ttgactcgct gttccttgat
    38821 gaaggttttg gcacgctgga tagcgaaacg ctggataccg cccttgatgc gctggatgcc
    38881 ctgaatgcca gtggcaaaac catcggtgtg attagccacg ttgaagcgat gaaagagcgt
    38941 attccggtgc agatcaaagt gaagaagatc aacggcctgg gctacagcaa actggaaagt
    39001 gcgtttgcag tgaaataact attcagcagg ataatgaata cagaggggcg aattatctct
    39061 tggccttgct ggtcgttatc ctgcaagcta tcactttatt ggctacggtg attggtagcc
    39121 gttctggtgg ttgtgatggt ggtatgaaaa aagtcatttt atctttggct ctgggcacgt
    39181 ttggtttggg gatggccgaa tttggcatta tgggcgtgct cacggagctg gcgcataacg
    39241 taggaatttc gattcctgct gccgggcata tgatctcgta ttatgcgctg ggggtggtgg
    39301 tcggtgcgcc aatcatcgca ctcttttcca gccgctactc actcaaacat atcttattgt
    39361 ttctggtggc gttgtgcgtc attggcaacg ccatgttcac gctctcttcg tcttacctga
    39421 tgctcgccat tggtcggctg gtatccggct ttccgcatgg cgcatttttt ggcgtcggcg
    39481 cgatcgtgtt atcaaaaatt atcaaacccg gaaaagtcac cgccgccgtg gcggggatgg
    39541 tttccgggat gacagtcgcc aatttgctgg gcattccgct gggaacgtat ttaagtcagg
    39601 aatttagctg gcgctacacc tttttattga tcgctgtttt taatattgcg gtgatggcat
    39661 cggtctattt ttgggtgccg gatattcgcg acgaagcgaa aggaaagctg cgcgaacaat
    39721 ttcacttttt acgcagcccg gccccgtggt taattttcgc cgccaccatg tttggcaacg
    39781 caggtgtatt tgcctggttc agctacgtaa agccatacat gatgtttatt tccggttttt
    39841 cggaaacggc gatgactttt attatgatgt tagttgggct agggatggtg ctggggatgg
    39901 tgctggggaa tgtgctaagt ggccggattt caggacgtta ttcaccactg cgcattgcgg
    39961 cagtaactga ctttattatt gtgctggcac tgctgatgct ctttttcttc ggcggcatga
    40021 aaacaacgtc gcttattttt gcatttattt gttgcgcggg attatttgcc ctttcagcac
    40081 cactgcaaat attgttactg caaaatgcca aaggcggaga gttattaggt gccgcaggtg
    40141 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggtggt atgatgctga
    40201 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    40261 tgtcgtcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    40321 tggcgaaacc actggggtag attactgtca ccgtgcgcgt tgcttattgc cggatgcggc
    40381 gtaaacgcct tatccggcct acgggctgct cattttgtag gcctgataag acgcggcaag
    40441 cgtcgcatca ggcatgatgc gctaattgcc tggttttttt actcttgtgg ccataaccac
    40501 gcagcgccgc gtacgccgct ggaatcaccg tgcttcgcct tacgcaccgg cgtttcacat
    40561 tcgccgccga agacaaattg tttaatcaac tgcggaaccg tttgatataa acggtctaca
    40621 ttgctcatcc cgccccccag gacaatcaca tccggatcga gaatattcac gacatgtgcc
    40681 agcgattttg ccagccgcag ctcgtagcga cgcaatgcca gttccgctac cggatcgctt
    40741 tcttcaacca ggcggataat ttcactgcct ttcagcgcat gtccgctcaa acgacgataa
    40801 tccgtcgcaa atcccgtgcc cgaaataaag gtttcaatac aaccttgttt accgcaataa
    40861 caagggactt cctcgcgata acgcagttcg tcttcgtcca tccacggtag cggattgtgt
    40921 ccccactcac ctgccgtgcc attgccgccg atatgcgccc gcccattgaa tgccacgccc
    40981 gcgccgcatc ccgtgccgat aatcacggca aataccgtct gcgctcccgc tgccgcgcca
    41041 tctactgctt ctgaaaccgc cagacagttg gcgtcatttg ccagccgcac ttcccgctgc
    41101 aacctcgcgc ttaagtcttt atcgaatggc tgaccgttga gccaggttga attggcattc
    41161 ttcaccacgc cggtgtaggg cgaaatcgaa ccaggaatgc ccatacctac cgttccgcgc
    41221 tgccctgttg cctgctccgc catatcaacc aacgtggcga tcgtttcaat agtctgccga
    41281 taatcatcac gcggcgtggg cagacgatgg cggtacaact gctcccctgc atcgcccagt
    41341 gcaatcactt cagttttggt gccgcctaaa tcgataccta tacgcacggt actctcctta
    41401 tttttttcaa tatcaatagc gtagagacgg acaactggat tggcaatgca aggccgccga
    41461 caattcgtta tcatgcccgc taaatttaac gacaaggccg tggaaattat catgctgtgg
    41521 ttcaaaaatt taatggttta ccgtcttagc cgcgagattt cgttgcgtgc agaagagatg
    41581 gaaaaacagc tagcctcgat ggcatttacc ccatgcggca gccaggacat ggcgaagatg
    41641 ggctgggttc ctccgatggg atcgcacagc gatgcgttaa cgcacgttgc caatggtcaa
    41701 attgttatct gcgcgcgcaa agaagaaaaa atcctcccgt ctccggtgat taaacaggcg
    41761 ctggaagcga aaatcgccaa actggaagcg gaacaggcgc gtaagctgaa gaaaaccgaa
    41821 aaagattcgc tgaaagacga agtgctgcac tctctgctac cgcgtgcttt cagccgtttt
    41881 agccagacaa tgatgtggat cgacacggtt aacggtttga ttatggtgga ctgcgccagt
    41941 gccaaaaaag cggaagatac gctggcactg ctgcgtaaaa gcctggggtc gttaccggtt
    42001 gtaccattga gcatggaaaa cccgattgaa ctgacgctga ccgaatgggt tcgctccggc
    42061 agcgcggcac agggcttcca gctgcttgat gaagccgagc tgaaatcgtt gctggaagat
    42121 ggcggagtga tccgcgcgaa gaaacaagat ctgaccagcg aagagatcac caaccacatt
    42181 gaagccggaa aagtggtgac taaactggcg ctcgactggc agcagcgcat tcagtttgtg
    42241 atgtgcgacg atggttcgct caagcgtctg aagttctgcg acgagctgcg cgatcaaaac
    42301 gaagatatcg accgtgaaga tttcgcccag cgctttgatg ccgatttcat cctgatgact
    42361 ggtgaactgg cagcgttaat tcaaaacctg attgaaggat taggtggcga agcacaacgt
    42421 taattgctga tttaccttta atgccggatg cgacgcctgc cgcgtcttat ccggcgtacg
    42481 aagccacacc aggaatataa tcgttagcta cagcgggcaa taattcttag cgcagcaata
    42541 ttatgcgttt tatgctgtaa cttgctccag gggcgttgtg tcattgtttt tcctcaagcc
    42601 ggtgacgcgc ttcactgcga atttttcgcg ttgccccgtt tttcactaaa tcacgtaaca
    42661 gcgaaagatc gggttgttcg acaaataaga gtaatgatgg ctcctctttt aaccacgccg
    42721 ttttcacatc ggcagccagc tgtgaattat taatagccag cgaccgctcc tgaggctcag
    42781 tcaatgtcgt taacaacacc ggagtatgtg cgttgtgcaa tgccgtttgg cgcaaatgat
    42841 cattgcgact ttgcagcaat gcacaccacc attcatcatc tgatttatct tttaacgtcg
    42901 ccagttccgc aatagtatct ggagatcttc gctgatattt atatcaacct tgatgaatta
    42961 tgaatatatc ggaaatcgaa aaaatcccag caaaggaaat attattatgg gttaaagaga
    43021 ggacatttca gaaaaatgcc ctcccgttca aggggaaggc gaggaattac agatagcggc
    43081 acagataaga ggtgggttcg gcaacttgca gatgaaactc gctgtgaccg ggaacattaa
    43141 acagcgaacc ggcttcatac acttgccagt cggtcgcgtc aggcagtaac acattcagcg
    43201 cgccactgat taccgtcatc tcttcaggct cagcggtgct gaaggtgtat tcaccttcaa
    43261 ccataacacc cacgctggcg cgaccagtgc tgctgctgga aaagccgatc gatttcactt
    43321 tgccggaaaa gtactcatta ctttgaagca taaactggcc ctgtctggta gttgaacatc
    43381 tacttcacta taggggccag aggcgctggc tgtcacgcaa aattacatca gtaattccgc
    43441 agccagccgt gcaatcaata cgttagagag caagacagga acatcgagct gtttttgcag
    43501 taaatcgcga tgacgctggt taaatcccaa acaatccagc atgatgacat ctgcaccttt
    43561 tgccagtaat tctttcccgg catcaatgat tttttgttcc gaatcatgaa tgggattacc
    43621 caatgaaaat actggcggtt tctgcaaaat ttgccatttt tgcgcctgaa cggtcagcaa
    43681 ctcctcaacc ggtacgataa cccccacctg atgatcttca acaatagagg aaaccagcgg
    43741 tggcaatatc cgcgacggct caagaaagat cgtattacgc gcggtcatac tactaatgtt
    43801 tgctgtactc attaatataa tgacgtcgta accctgatta tcgagcactt caaccacacc
    43861 ttgcaggtct cgctccactt tgcgacgcga aacatgggcc agatggttgt catttaataa
    43921 tgtgagaatg gtgtcttcgc ctgcttctgg cgcgtactcc gccatcactt cttcgcggct
    43981 taacttcccc agcaggctat gatgggaaat attatcttcg tcaatgtatt ctgtcaggag
    44041 cggcaaaact tcctgcatag gtacaatgcc gatggtcagg atcgccaacg acgcactcat
    44101 tgttcttcca ccttactttt atttcgtcgt taattaccgg gcaagtgtta agcaccactg
    44161 ctgacactta cttgtgctac aaatgacaaa aagcgtagca gcagacctgc actgcataat
    44221 gtaaagattc caaatgattc cagtaatgga ttgttattgt ttaatattct aattatttga
    44281 aaaacatgaa ttgtgaaaaa tgtgacgcag atcccttgtg gcggcactca gaatcccttc
    44341 cgagcagtct gatgcggtca ctctttgtca gggtcctcat agcgtttttt cgcatccatc
    44401 gcttcctgag cggtcggatg ttcactgatc aacgagtctg gtttaggatg atcggctctg
    44461 agttggtacc aggttaccgt ctgtcctggc tttccttttt caatcgtcac tatatatgct
    44521 tcacgaggat aaggcggttt cgttggcatc gttcttcctt atttcacggg atgaacgtta
    44581 agtataggcg ctcgaaaatc aacaattgat cgtctgtgcc agagcgctgc gaatttcaga
    44641 aatcacctgg ctgggttcgt ttgttgcgtc gatgataata tgcgcaactt cgcgatatag
    44701 cgcatcgcgt tcttccagca cttcctgaac ttcttcgctc agtggttttc ccgttaaggt
    44761 tggccgtaaa tcttcttccg gtgcagcttg cagtcggtta accaggactg atactggcgc
    44821 acacaaataa accacgatcc cgttattttg catgaagtga cgattaaatt ccgtcagaat
    44881 aatgccgccg cctgtagcga taacggtgga tggcgcagtt accgcttcca gcgccgccgt
    44941 ttctctggcg cggaatcccg cccactcttc cctttcgacg atctccgcga ccgtcatatt
    45001 gagctgtgat tgcaaccact ggtcggtatc gacaaaccga cggttaagcg aatcggcaag
    45061 ggccattccg accgttgttt taccacagcc ccgaggcccg atcagaaaaa gaggttgtgt
    45121 catcgtgggt tttccccaat aggtcgcaat gcggcgaaag ccggtgtcat gagaatagcg
    45181 atcataccat caaactagta caatttcgat tgtaaagaaa aaattccact taaagtgaaa
    45241 atctcaatac atcccttact ataccaataa atattcaaga atgaagtgta aataataaat
    45301 tacatttagc cacgactacg ttgcacttcc agccaccact tctccagctc cgcagcaaaa
    45361 gcctggcggt cacgttgtga aagactatct ggtcctccgg tctggatccc actggcacgt
    45421 aaggtatcca taaaatcgcg catcgtcagg cgctcacgaa tggtcgctgg cgtgtaacgt
    45481 tcgccgcgcg gattaagcgc cgccgcgcct ttctcgatgg cttcagcagc caaaggtata
    45541 tctgcggtga tcaccaaatc gcccgcttca cactgccgaa caatttcgtt atcagcaacg
    45601 tcgaaacctg ccgcgacgcg cagcgtacga ataaatcgcg atggcggcac gcgtaaactc
    45661 tggtttgcta ccagtaccag cggcatctgc atacgttccg ccgcgcgata caaaatctct
    45721 ttaattacat tgggacacgc gtcggcatcc acccaaattg tcataaagtc atcctttgtt
    45781 gggtaatcct ctattgtgtc gcgcttttgc cttccggcat agttctgttt atgcttctgc
    45841 cagcgattat caaaacaatg aatttcacgg caggagtgag gcaatggaaa agaaaatcgg
    45901 ttttattggc tgcggcaata tgggaaaagc cattctcggc ggtctgattg ccagcggtca
    45961 ggtgcttcca gggcaaatct gggtatatac cccctccccg gataaagtca ccgccctgca
    46021 tgaccagttc ggcatcaacg ccgcagaatc ggcgcaagaa gtagcgcaaa tcgccgacat
    46081 catttttgcc gccgttaaac caggcatcat gattaaagtg cttagcgaaa tcacctccag
    46141 cctgaataaa gactctctgg tcgtttctat tgctgcaggt atcacgctcg accagcttgc
    46201 ccgcgcgctg ggccatgacc ggaaaattat ccgcgccatg ccgaacactc ccgcgctggt
    46261 taatgccggg atgacctccg taacgccaaa cgcgctggta accccagaag ataccgctga
    46321 tgtgctgaat attttccgct gctttggcga agcggaagtg attgctgagc cgatgatcca
    46381 cccggtggtc ggtgtgagcg gttcttcgcc agcctacgtc tttatgttta tcgaagcgat
    46441 ggccgacgcc gccgtgctgg gcgggatgcc acgcgcccag gcgtataaat ttgccgctca
    46501 ggcggtcatg ggttccgcaa aaatggtgct ggaaacggga gaacatccgg gaacactgaa
    46561 agatatggtc tgctcaccgg gaggcaccac cattgaggcg gtacgcgtac tggaagagaa
    46621 aggcttccgt gccgcagtga tcgaagcgat gacgaagtgt atggaaaaat cagaaaaact
    46681 cagcaaatcc tgatggcttt tgccggacgt caggccgcca cttcggtgcg gttacgtccg
    46741 gctttctttg ctttgtagag cgccaaatct gccgatttca accactcgcg atagtgactc
    46801 atttgtggat tcagcggcgc aacccccaca ctaatccgta aagttacctg tggcgtattc
    46861 ggcaagcgta acgtgtttag cccttcatgc acccgtaaca tggcggtgat ggcgctctct
    46921 gctggcgtac cggacatgat tactgcaaac tcatcgccgc caaaccgacc aatcacatcg
    46981 ctaccgcgta gggtaatttg taactgtcgg gtaagcgcca caatcgcttc atcacccaca
    47041 tcatggcccc aggtatcgtt gatgctcttg aaatggtcga tatcgataat cagtaacgtt
    47101 gcatcgcgat tatgccgccg acagttatca aattcattgc gtaacatagt ttcccaatga
    47161 cgtcggttat acacgcccgt cataccgtcg cgggtactca tgacctgcaa cctgcgttta
    47221 tgttccgcca gtttagtcgc cgtctggtag ctgacccagc caaacagcag aggataaatg
    47281 acaataatgg gaagggagag ccaccattcc agcggtgcgc tattgaacga cacggtgatg
    47341 cccgtcagct cgagggtgac aaggcaggag accaccatca acaccagacc cgcgacaaac
    47401 agacgggggc cgcctgcccc catcaaattc agacacataa tcatcaacat cgcggtggaa
    47461 ggcagtgcgt ttacgcccat tacgcctacc cacattcccg ctaatactgc atcggttttt
    47521 aagttgtaaa tttcccggct aagcggatcg acggccctgc tcgctatttg ccaggctaaa
    47581 tgcggccaga cgaacgccca gccgaccaac aacagccacc accagcccgg cgacggatgc
    47641 gaaaccagcg ttgaagcaat cggtaagaac atgccagcca ggccaaccgc gcggggtagt
    47701 ctgacgcgac gggcaaagcg cagcccggac cgctgatgtt cgttttgagg agttaaagga
    47761 ggttcctccc catgcgccgc cgcttttttg aaaaagtttt catcattcat tatttttggg
    47821 aacattcaga aacaattttc ccaaattata gagacggggc taaaggcaga agtatgaaat
    47881 ttcgggtgag ccattttcgt agctcacccg ttagcactat caggtatcag gcggctttct
    47941 tgaggcaggc gctcataaac ttattacgat catccccttt cagagattgt tgcgttgctt
    48001 gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa acttttttca ccaggcgcag
    48061 acttgctgtt cttcaggcaa tcactcatgt aggtcttacg agcatcccct ttcaacgcct
    48121 gcgccgtcgc ctgctgatta caggaggtca tacgctgttg ttgcggggtt aaggttctct
    48181 cggcagcgcc gacggtggtt aaaaaaacca gtccgaaaag caaggtaacc agtaatgtta
    48241 ttttcatcgc accatccctc ttcatgtttt aaccatgagc gtttgcgccc gtgatctgcc
    48301 attaagtctg gttgctaaca gcaaaaaaac cacccgacag cgaaaattca ccgccgggcg
    48361 cggttttatt tcagccccag ggcggctttc atggtgtaga agagatcggt ctggtcggtc
    48421 agtccaacga cattggcggc atgtgggcca tacgctgcaa tacgcaactg actgccggta
    48481 tgttcttgtg aatcctcttc ggagttcccg taactcatca ccatcactgc gccatctttg
    48541 gtatttagcg cctgggtgag gcccggcgct ttggtgtccg gcgcgacaat ctggctggcg
    48601 tgggcgtgat cagcggtgac tatgaccagc gtgttgccat cctttttagc gaattccagc
    48661 gcacgttgta cggcttcatc gagatcgacc gtctcgccaa tttgcccaca aggattcgca
    48721 gcgtgatcct gtttatcgat ggatgcacct tcaacttgca ggaaaaaacc tttctcattt
    48781 ttactcaaca attcaatggc tttgtcggtc atctgcgcca gggtcggtac actgtcatta
    48841 cgttgcggat taggcgtaca ggtaactgcg ggcttatcga tattgccgtg gtacgttgct
    48901 ttcggtccta gccagcgcac tggcatattg ccgtcagcaa acaggccaag caggggtttt
    48961 tgctgattcg cttccgtcac cgcatttagc gaggcagcat cactcaccaa ctgataacca
    49021 cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt cgccgacggt tgccgtttca
    49081 gcaaaggttt ttgcgccgcc gccaagcgta acatcggcac gagcgttaag cagctgttcg
    49141 gtaatcgatc cttttccgcc tttttccaga gcgttacccg gacatttttc actggtcgcg
    49201 ctcggaccgt agcatttgcg cgaggtcaca tgcgccacca gcgcggcggg cgtggcatcc
    49261 tgcaactctg cggtagaaac gttaccggtc gccagacctg cggcttttgc catttccaga
    49321 atcgttgggt gatctttttc gtgaatatcg acgcccagcg caccgttata ggttttgaca
    49381 ccagttgacc aggcggttgc tgatgcagcc gagtcggtga cgtagtccgg tttaccggtt
    49441 tttttattca gcgcatagtg agtgtattgc ccggtaagcg gtaatgcatc gataccttta
    49501 aaaaagccgc ccgcaccttc ggcataattg cgtgcagcag taatttccga gtcccccatc
    49561 ccatcgccaa tcagcaaaat aatatttttg gcgggtttat cgctaagaga atcacgcaga
    49621 gcggcggtct gatcccccgt taaacggcga gcaccgccgg gtgcagtaat atcgccctga
    49681 gcagcccggt tttccagaac aggcatttct ggtgtccggg cttttgtcac aggggaaaac
    49741 agtaacggta agagtgccag tgcaatagtg ctttgtttca ctttattttc tccatgtaca
    49801 aatacattaa aaataaaaac aaagcgacta taagtctcgg ccgtgacagc tttatgacag
    49861 ctgttgaaaa gattaacttt ttactgacga ggatgcttca ataacttctt tacataattg
    49921 cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg gctttacctc gtacagcgcc
    49981 ccctctacct gatcaatcaa cctctgttgg tcattttgcg ccatattgcg aagcattgca
    50041 gtgacgataa tctccaaagc ttctacctgc gcgcacagtt ctttcgactc ttcttctttt
    50101 tgggcaagct taaataacaa ctcagcaatg agatttttca tgtctgtatt tccttatcca
    50161 aagtatggag aagttaacat tatgttt
//
LOCUS       DAZUCC010000009        48203 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZUCC010000009 DAZUCC010000000
VERSION     DAZUCC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.009"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            154..1371
                     /gene="alaA"
                     /locus_tag="ACHY09_000442"
     CDS             154..1371
                     /gene="alaA"
                     /locus_tag="ACHY09_000442"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFS3883459.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1455..2054
                     /gene="yfbR"
                     /locus_tag="ACHY09_000443"
     CDS             1455..2054
                     /gene="yfbR"
                     /locus_tag="ACHY09_000443"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFS3883460.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2113..3945)
                     /locus_tag="ACHY09_000444"
     CDS             complement(2113..3945)
                     /locus_tag="ACHY09_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFS3883461.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4032..4682)
                     /gene="hxpA"
                     /locus_tag="ACHY09_000445"
     CDS             complement(4032..4682)
                     /gene="hxpA"
                     /locus_tag="ACHY09_000445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFS3883462.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4693..5187)
                     /locus_tag="ACHY09_000446"
     CDS             complement(4693..5187)
                     /locus_tag="ACHY09_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS3883463.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5271..5726)
                     /gene="yfbV"
                     /locus_tag="ACHY09_000447"
     CDS             complement(5271..5726)
                     /gene="yfbV"
                     /locus_tag="ACHY09_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFS3883464.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6064..7266
                     /gene="ackA"
                     /locus_tag="ACHY09_000448"
     CDS             6064..7266
                     /gene="ackA"
                     /locus_tag="ACHY09_000448"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFS3883465.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7341..9485
                     /gene="pta"
                     /locus_tag="ACHY09_000449"
     CDS             7341..9485
                     /gene="pta"
                     /locus_tag="ACHY09_000449"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS3883466.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9675..11195
                     /gene="yfcC"
                     /locus_tag="ACHY09_000450"
     CDS             9675..11195
                     /gene="yfcC"
                     /locus_tag="ACHY09_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS3883467.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11228..11770)
                     /gene="yfcD"
                     /locus_tag="ACHY09_000451"
     CDS             complement(11228..11770)
                     /gene="yfcD"
                     /locus_tag="ACHY09_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFS3883468.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11828..12379)
                     /gene="yfcE"
                     /locus_tag="ACHY09_000452"
     CDS             complement(11828..12379)
                     /gene="yfcE"
                     /locus_tag="ACHY09_000452"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFS3883469.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12435..13079)
                     /gene="yfcF"
                     /locus_tag="ACHY09_000453"
     CDS             complement(12435..13079)
                     /gene="yfcF"
                     /locus_tag="ACHY09_000453"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFS3883470.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERSTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13215..13862
                     /gene="yfcG"
                     /locus_tag="ACHY09_000454"
     CDS             13215..13862
                     /gene="yfcG"
                     /locus_tag="ACHY09_000454"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFS3883471.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAASQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13919..14281
                     /gene="folX"
                     /locus_tag="ACHY09_000455"
     CDS             13919..14281
                     /gene="folX"
                     /locus_tag="ACHY09_000455"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFS3883472.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14302..15195
                     /locus_tag="ACHY09_000456"
     CDS             14302..15195
                     /locus_tag="ACHY09_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFS3883473.1"
                     /translation="MKIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VALWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCAHWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15243..16145)
                     /gene="rpnB"
                     /locus_tag="ACHY09_000457"
     CDS             complement(15243..16145)
                     /gene="rpnB"
                     /locus_tag="ACHY09_000457"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFS3883474.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLE
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16342..17115)
                     /gene="hisP"
                     /locus_tag="ACHY09_000458"
     CDS             complement(16342..17115)
                     /gene="hisP"
                     /locus_tag="ACHY09_000458"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFS3883475.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17123..17839)
                     /locus_tag="ACHY09_000459"
     CDS             complement(17123..17839)
                     /locus_tag="ACHY09_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3883476.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17836..18522)
                     /gene="hisQ"
                     /locus_tag="ACHY09_000460"
     CDS             complement(17836..18522)
                     /gene="hisQ"
                     /locus_tag="ACHY09_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFS3883477.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18612..19394)
                     /gene="hisJ"
                     /locus_tag="ACHY09_000461"
     CDS             complement(18612..19394)
                     /gene="hisJ"
                     /locus_tag="ACHY09_000461"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFS3883478.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19615..20397)
                     /gene="argT"
                     /locus_tag="ACHY09_000462"
     CDS             complement(19615..20397)
                     /gene="argT"
                     /locus_tag="ACHY09_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFS3883479.1"
                     /translation="MKKSILALSLLVGLSAVASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20663..21232)
                     /gene="ubiX"
                     /locus_tag="ACHY09_000463"
     CDS             complement(20663..21232)
                     /gene="ubiX"
                     /locus_tag="ACHY09_000463"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFS3883480.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21327..22844)
                     /gene="purF"
                     /locus_tag="ACHY09_000464"
     CDS             complement(21327..22844)
                     /gene="purF"
                     /locus_tag="ACHY09_000464"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS3883481.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22881..23369)
                     /gene="cvpA"
                     /locus_tag="ACHY09_000465"
     CDS             complement(22881..23369)
                     /gene="cvpA"
                     /locus_tag="ACHY09_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFS3883482.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23628..24278)
                     /gene="dedD"
                     /locus_tag="ACHY09_000466"
     CDS             complement(23628..24278)
                     /gene="dedD"
                     /locus_tag="ACHY09_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFS3883483.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAVNNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPASKPVVEEKAAPTGKAYVVQLGALKNADKVNEIVGKL
                     RGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYTPN"
     gene            complement(24268..25536)
                     /gene="folC"
                     /locus_tag="ACHY09_000467"
     CDS             complement(24268..25536)
                     /gene="folC"
                     /locus_tag="ACHY09_000467"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFS3883484.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYTSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25606..26520)
                     /gene="accD"
                     /locus_tag="ACHY09_000468"
     CDS             complement(25606..26520)
                     /gene="accD"
                     /locus_tag="ACHY09_000468"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS3883485.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26676..27335)
                     /locus_tag="ACHY09_000469"
     CDS             complement(26676..27335)
                     /locus_tag="ACHY09_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFS3883486.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27418..28230)
                     /gene="truA"
                     /locus_tag="ACHY09_000470"
     CDS             complement(27418..28230)
                     /gene="truA"
                     /locus_tag="ACHY09_000470"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS3883487.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28230..29243)
                     /locus_tag="ACHY09_000471"
     CDS             complement(28230..29243)
                     /locus_tag="ACHY09_000471"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS3883488.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29309..30445)
                     /gene="pdxB"
                     /locus_tag="ACHY09_000472"
     CDS             complement(29309..30445)
                     /gene="pdxB"
                     /locus_tag="ACHY09_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFS3883489.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDDTWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTRHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30544..31539
                     /gene="flk"
                     /locus_tag="ACHY09_000473"
     CDS             30544..31539
                     /gene="flk"
                     /locus_tag="ACHY09_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFS3883490.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31536..32714)
                     /locus_tag="ACHY09_000474"
     CDS             complement(31536..32714)
                     /locus_tag="ACHY09_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3883491.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(32979..34199)
                     /gene="fabB"
                     /locus_tag="ACHY09_000475"
     CDS             complement(32979..34199)
                     /gene="fabB"
                     /locus_tag="ACHY09_000475"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFS3883492.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34358..36364
                     /gene="mnmC"
                     /locus_tag="ACHY09_000476"
     CDS             34358..36364
                     /gene="mnmC"
                     /locus_tag="ACHY09_000476"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFS3883493.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHLHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RLYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVGANEVEGVTGVV
                     TNCGGITYPQGGWLCPAELTCNVLKLAQQQGLQIRYQHQLKDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSEKEARCGV
                     RCATRDHLPMVGNVPDYDVTLVEYASLAEQKDEAVSAPIYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSNEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36420..36698)
                     /locus_tag="ACHY09_000477"
     CDS             complement(36420..36698)
                     /locus_tag="ACHY09_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFS3883494.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQKASQQ"
     gene            complement(36732..37280)
                     /gene="epmC"
                     /locus_tag="ACHY09_000478"
     CDS             complement(36732..37280)
                     /gene="epmC"
                     /locus_tag="ACHY09_000478"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFS3883495.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37280..38089)
                     /locus_tag="ACHY09_000479"
     CDS             complement(37280..38089)
                     /locus_tag="ACHY09_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS3883496.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38089..38913)
                     /gene="mepA"
                     /locus_tag="ACHY09_000480"
     CDS             complement(38089..38913)
                     /gene="mepA"
                     /locus_tag="ACHY09_000480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFS3883497.1"
                     /translation="MNKTAIVLLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38916..40001)
                     /gene="aroC"
                     /locus_tag="ACHY09_000481"
     CDS             complement(38916..40001)
                     /gene="aroC"
                     /locus_tag="ACHY09_000481"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS3883498.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40036..40968)
                     /gene="prmB"
                     /locus_tag="ACHY09_000482"
     CDS             complement(40036..40968)
                     /gene="prmB"
                     /locus_tag="ACHY09_000482"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS3883499.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            41134..41685
                     /gene="smrB"
                     /locus_tag="ACHY09_000483"
     CDS             41134..41685
                     /gene="smrB"
                     /locus_tag="ACHY09_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFS3883500.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41801..42643)
                     /locus_tag="ACHY09_000484"
     CDS             complement(41801..42643)
                     /locus_tag="ACHY09_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFS3883501.1"
                     /translation="MKKWTMIFASLVLLVMSASVPKSYAGDKLTSASFDSVKIYYGKG
                     KMSIHNTGGLYVTVLTPNEAAYGVYNDAIRKNEQASVISWTGSGPAPVLILTDYGNAD
                     SSNCPGINTATFQCAYETFKITVQSDSYGCPWLASFYTITDQPGFGRYTGPTNHATVC
                     PTIPVASYDISWSENYVSHNKALQLQSNGSTITTTLSTYLMEDGKLCDGSVFDSRGAY
                     CRVVSELLTFTSYGCDKSTVTVSPTRHPVTDKQLHDIVVNVNTSSRQPIDSTCRFQYV
                     LNEL"
     gene            complement(42645..43169)
                     /locus_tag="ACHY09_000485"
     CDS             complement(42645..43169)
                     /locus_tag="ACHY09_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3883502.1"
                     /translation="MKKKRALVFICSLMLLYSVTAPAVDNLHFTGNLLGKSCTPVING
                     SLLAEVHFPTIAASDLMNLGQSERVPLVFQLKDCKSSTLFSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGAAVPVNSTTGVTFPLNQGNNSLNFNAWLQAKGGRDVTS
                     GDFSATATATFEYF"
     gene            complement(43166..43636)
                     /locus_tag="ACHY09_000486"
     CDS             complement(43166..43636)
                     /locus_tag="ACHY09_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3883503.1"
                     /translation="MKRISLLMLWGFSSMALSNVSFHGYLVQPPNCTISNGKTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMKLSWSGTQSDFDDAAVSTDISGLA
                     IRLKQAGQSFKLNTALVVNETALPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            complement(43633..44139)
                     /locus_tag="ACHY09_000487"
     CDS             complement(43633..44139)
                     /locus_tag="ACHY09_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3883504.1"
                     /translation="MKRSCSVWAGTWLLMCCIGSDFAVAGSKSVNLSLRVLVDAPPPC
                     TVNGATVEFGNVFINKIDGVGYKQPIDYSLVCNNLAMDDLRLQMRATTVVINGETVIS
                     TGISGFGIRVQKSSDHSTLDLTSGSWLPFNFSSGVPALEAVPVKQSGTTLSAAEFNAS
                     ATIVVDYQ"
     gene            complement(44156..44905)
                     /locus_tag="ACHY09_000488"
     CDS             complement(44156..44905)
                     /locus_tag="ACHY09_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS3883505.1"
                     /translation="MTKHLSVKTLATVLSLLAATVSFSALASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKQPYLAQSWIEDEKGVKISSPLAVLPPVQRIDAMMNGQVKIQGLPDIH
                     KLPADRESIFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALEKASMQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPADGFSPIVMSPKTSAPLKVKMASVPVL
                     TYVNDYGARMPLFFTCSGNSCQVDEELSRKG"
     gene            complement(44925..47564)
                     /locus_tag="ACHY09_000489"
     CDS             complement(44925..47564)
                     /locus_tag="ACHY09_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFS3883506.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNVLADDTIQFDARFLELKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNHPLPDEYDIYWYVAENDPNKSYACLPPELI
                     AQFGFKDDFAKSLQWGHDGQCLKTDQIGGMEIKGDLSQSALLVSVPQAYLEYTDDDWD
                     PPSRWDEGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNMRGYAPDISGVARTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALFGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDTDRARTGNDKEMY
                     TVTYNQNFRDARVSVYLNYSHHTYWDREDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSSQASLYHRIDDASHYTLSAGTS
                     ENHTSLDGYYSHDSTLAKVDLSANYHEGQYTSAGVSLQGGVTLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATQ
                     SVVQATLTEGAIGYRKFAVISGEKAMVVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSAPVITHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            complement(47650..>48203)
                     /locus_tag="ACHY09_000490"
     CDS             complement(47650..>48203)
                     /locus_tag="ACHY09_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3883507.1"
                     /translation="KAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCSIVPDD
                     HKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMTTTFTGNPYSGNSKNYS
                     LSNMDNGTEIPNVSLVIGDQRGTGYDLGAGIDQDIAMDSSTGKGKQKQTLNFKAWLVG
                     ETAAVTPTPAPFETLTTFQITYL"
BASE COUNT        11883 a        12993 c        11929 g        11398 t
ORIGIN      
        1 gccattatta agttattaac agcacaatcg agtcttcccc tctggcaaaa tcttattctg
       61 cagaccttca aaacgccgtc ctgggggagt acattgttct aagctgactt ccacggcagg
      121 gagtggcgat aacagcaaaa aaggtcaaga ttcatgtccc ccattgaaaa atccagcaaa
      181 ttagagaatg tctgttacga catccgtggt ccggtgctga aagaagcaaa acgcctggaa
      241 gaagaaggta acaaggtact gaaactgaac atcggcaacc ctgccccgtt cggttttgac
      301 gcgccagatg aaatcctcgt tgacgtgatt cgcaacctgc ctaccgctca agggtattgc
      361 gattccaaag ggctttactc cgcgcgtaaa gccatcatgc agcactacca ggctcgtggc
      421 atgcgtgatg ttaccgtgga agatatttac atcggaaatg gtgtatcgga gcttatcgtt
      481 caggcaatgc aggcattgct gaacagcggc gacgaaatgt tggttcctgc acctgattac
      541 ccactatgga ccgcggcggt ttcgctctcc agcggtaaag cggtgcatta tctttgcgat
      601 gaatcctctg actggttccc ggaccttgat gatattcgcg ctaaaattac gcctcgtacg
      661 cgtgggatcg ttattatcaa cccaaataac ccaaccggcg cggtatattc caaagagctt
      721 ttaatggaga tagtggagat tgcacgtcag cataatctca ttatcttcgc cgatgaaatt
      781 tatgacaaaa ttctctacga cgacgctgag catcactcaa ttgcgccgct ggcacctgat
      841 ctgctgacca ttacctttaa cggcctgtcg aaaacatacc gcgttgcagg cttccgtcag
      901 gggtggatgg tgttgaacgg gccgaaaaaa cacgccaaag gctacatcga aggtctggaa
      961 atgctggctt caatgcgcct gtgtgctaac gttcctgcgc aacacgccat tcagaccgcg
     1021 ctgggcggtt atcagagcat cagtgaattt attacccctg gcggtcgtct ttatgagcag
     1081 cgtaaccgcg catgggaatt gatcaatgat attccgggcg tttcctgcgt gaaacctcgt
     1141 ggtgcgctgt acatgttccc gaaaatcgac gccaaacgct ttaatattca cgacgatcag
     1201 aaaatggtgc tggatttcct gttgcaggaa aaagttctgt tggtgcaagg gacggcattc
     1261 aactggccgt ggccggatca cttccgcatt gtcacgctac cgcgtgtcga tgatatcgag
     1321 ctgtctttga gcaagttcgc gcgtttcctt tctggttatc atcagctgta atcttaattt
     1381 cactgccgga gattgcatcc ggcagtgtta tcccgccaca atgacctgat gatgtcatca
     1441 tacgtaaggt cactatgaaa cagagccatt tttttgccca tctctcccgc ctgaaactca
     1501 ttaaccgctg gccgctaatg cgcaatgtgc ggacggaaaa tgtgtccgaa cacagtttgc
     1561 aggtagcgat ggtcgcccat gcgctggcag ctatcaaaaa tcgtaaattt ggcggtaatg
     1621 tcaacgccga acgtatcgct ttactggcga tgtaccacga tgcctcagaa gtgcttaccg
     1681 gcgatctccc taccccggtg aaatacttca attcgcaaat cgctcaggaa tacaaggcga
     1741 ttgaaaaaat cgctcagcaa aaactggtcg atatggttcc ggaagagttg cgggatatct
     1801 ttgcgccgtt aattgacgag catgcatata gtgatgaaga aaaatcactg gtgaaacagg
     1861 cagatgcact atgtgcatat ctgaaatgtc tggaagaact cgcggcggga aataatgaat
     1921 tcttgctggc aaaaacgcga ctggaagcga cgcttgaagc gcgccgcagc caggagatgg
     1981 actacttcat ggaagtcttt gttcccagct tccatctttc gctcgatgag attagccagg
     2041 attcaccgct gtaagcagcc ggagtctgcg tcgcatcagg caataagcgc cggatgcgac
     2101 atcaggctct tgtcaaaacg gaaacagcat cgggatcatc accacacaaa ccgccatcac
     2161 gataatggtg aacggtaccc ccaacttcac aaagtcactg aagctgtaat ttcccggacc
     2221 taaaaccagt gtgttaacag gtgaagaaac cggcgtcata aaggcggcgg atgctgccat
     2281 cgccacgacc atcgcgaatg ggtaaggcga cacccccatc gttttagcag cagccagcgc
     2341 aatcggagcc atcaacaccg ccgtcgcggt attagaaata aatagcccaa taaccgccga
     2401 caagacaaac aaacaaccca gcatcatatg tggcccgtaa ccgccgccaa tgtccatcag
     2461 ccctttcacc gccagcgcaa cacctcccgt tttctgtaat gccacagcaa acggcatcat
     2521 cccaacgatc aaaataatgc tcggccagtg tatggattta taggcgcttt cagcatcgat
     2581 acagcggaat ttccccatca gcaggcaggc gatgatagcg gcaacaggat taggaatttc
     2641 atcggtcagc attaacgcca ccattagcac cagacagaaa atggcatggg gtgcctgact
     2701 gtgcgcgggc gatgcttcac tcacctcttc cggtaagttc agcgctacga agtcgcggcc
     2761 ctgtttggcc agcataccga tcagtttcca gttacccaca accaggatga tatcgcccag
     2821 cagcagaggc tcatccgcca gcgaaccttc cagcgccacg ccattgcgct ttagccccac
     2881 tacattcaat ccgtagcggg tacgaaaacc aatttcgcgc accgatttac caataagttc
     2941 tgactcagga attaatgaaa tctctgccat gcccacatca agggcctggt cagaaaaata
     3001 ctcgccgcgc agtaccatcg gctccagcaa ttgctcacta caaaattgcc ggagatcgac
     3061 atcagccgca gacatatcaa taagcaaaac gtcacgcgcg cgaaattcag aaaccccatt
     3121 aacgttcacg ataacgcgac gaaaacgccg ccagcgttca acaccgatga cgttagcacc
     3181 ataacgttca cgtaatttga gatcgtccag tcgttgacca atcattggcg atccggggcg
     3241 aatagccaga cgtcgcgcac gcccggtcag tcgatattca cggataagat cgcgaaaggt
     3301 tcggcgcgtc cagccttcgc gttgtggggt ctgggtatcc cctttcagca tgaaacgcat
     3361 cactaacata tacaaaatac ccagcaccag cacgaccagg ccaataggtg ttacgctaaa
     3421 gaaactgaag ccgtgatagc cttcacgcag caattcactg ttgactacca ggttcggcgg
     3481 cgtcgccacc agcgtcatca tgccgcttat cagcccggca aaacttaacg gcatcatcag
     3541 acgcgacggc gacgtttgca tacgcatggc aacgcttaac accacgggaa taaagatagc
     3601 gacaacgccg gttgaactca taaacgcgcc aagccccgcg acggtcagca tcaacaaaac
     3661 caacattttg atttcactgt tgcccgccac tttgaccagc catgtcccca ttacagtggc
     3721 aacaccggta cggaccaaac catcgccaat aatgaacaag gcggcaatca ggacaacgtt
     3781 aggatcggaa aagccggaaa atacttcggg gaccgtcagc gttccgctta atgcaaacgc
     3841 gacaataaca aacaaagcga ccgcatccat acgcactctg cccgtcgcaa acaagacgat
     3901 ggcaacggcc agtaatgaaa gaacccaaat caattcaccg ttcacaactt atccttgtta
     3961 attgaggggg ataacttgat tctgccataa aaaagccccg acgagacggg gctaaatcat
     4021 gatcaggtgt ttcactgaat aataacatcg ccatttggct gtttggtcac agtaatttgc
     4081 tccagactgt ggaggaccaa atcgacctca ttcaggcgcg gggtatctgc cggagcgtta
     4141 accgcaatga catggcaacc cgccgccagg ccagaaagca cgccagcggg agcatcttcc
     4201 accactacac actcctgcgg cgcaagcccc agcagctgcg cgcctagcag atacgcatca
     4261 ggttctggtt ttccgcgctt cactcgctca gcggtcacaa acacctctgg cgcgggaagc
     4321 ccagctattt tatggcgcgc tcgcgctacc ggcatggagc cagaagtcac aatggcccac
     4381 ggaatacctg ctttattcaa atgattgagt aaggcgattg cccccggaag tgcggtaata
     4441 ccctcggttt ccgtggcctc gatttgctcc agacgcgtaa actcggcggc aatatcagcc
     4501 tcggatttgc ccgccataaa atggcgcaaa gaggtgatcg cctgtttacc gtgaataaaa
     4561 gccagcacct cttccggcgc taacccatga cgtctggccc agttgctcca cgcccgttct
     4621 accgcaggca gggaatccac cagcgttcca tcaagatcaa acagaaaacc tttgcaccgc
     4681 acgcgggcct cctcaggcgt tgataatttg attaatttcg ttggcgctca aatggtactg
     4741 acgcgggcag gcatgccaca cattaagcat gcgctgatat ttttcccaca ttggcgtctg
     4801 ggcgttaaaa ccgtgagttc cggcatcaaa atgggtatag cgtccttcca cattaaccat
     4861 aaagcggaca taaccgaggt aacgtgcttc agtggcggcg tcaaagccga ggaaggtgac
     4921 acgacgttca tcgatggatt gctggtcctg caaattagac caggaaacat gcaacgcatg
     4981 atacatctcc ataatgtcga tgatagtgcg gcaggtttct tctttcagct cgccaaactc
     5041 gcgatccagt tcacgcatct gtaatccata accacgctca atgattgttt gcaggcgacg
     5101 gtaacgttca gcatttgccg gatcgagcat agtcatcatc ttgtactggt tagacaaaat
     5161 aagacgttgc gcgttggtca tttccattgt tgactcctgt atcactctac tacggtgaaa
     5221 aaaaaagaag gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca
     5281 tcaaggaaag ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg
     5341 taatcaggct tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga
     5401 acttcataaa accagttaag gattgcaggg ggtaatggcg ttacagaacg tttgcccagc
     5461 caccacaatc cctgcatggg taaacttaag gcgaacagcg cagtggcaac tgccggccca
     5521 agctgaccgc ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa
     5581 cggatcgcat aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt
     5641 ttttccagcg gccacgtctt tgagtaatgc tgtccccggc gaaacaagct aaaaaaatta
     5701 acagaacgat tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa
     5761 atttgtgcaa attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca
     5821 ctatcaggta tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa
     5881 attcatatac attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt
     5941 tcttccatca tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat
     6001 catgcgctac gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact
     6061 tccatgtcga gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc
     6121 atcatcgatg cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg
     6181 cccgaagcac gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca
     6241 ggcgccgctc acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca
     6301 gaactgtctg cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat
     6361 accagctccg tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt
     6421 gcaccgctgc acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca
     6481 cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa
     6541 gagtcttacc tctacgccct gccgtacaac ctgtacaaag agcacggcat ccgtcgttac
     6601 ggcgcgcacg gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa
     6661 ccggtagaag aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct
     6721 atccgcaacg gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc
     6781 atgggtaccc gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg
     6841 ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg
     6901 accgaagtga ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg
     6961 aagcgcgcaa tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg
     7021 ctgatggatg gtcgtctgga cgctgttgta ttcaccggtg gtatcggtga aaatgccgcg
     7081 atggttcgtg aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa
     7141 cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct
     7201 gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact
     7261 gcctgatttc acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg
     7321 gtaacgaaag aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg
     7381 tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc
     7441 tgagcgtttt caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta
     7501 cgactatcgt gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct
     7561 acgttgaagg tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa
     7621 actaccacgc taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac
     7681 gtaagcacca gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa
     7741 tcgtcttcgt tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtattgaac
     7801 tgacccgcaa cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca
     7861 aactgaatgc accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg
     7921 acgactcttc caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc
     7981 cgctgccggt tctcggcgcg gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg
     8041 atatggctcg ccacctgaat gcaaccatca tcaacgaagg cgacatcaat actcgccgcg
     8101 ttaaatccgt cactttctgc gcacgcagca ttccacacat gctggagcac ttccgtgccg
     8161 gttctctgct ggtgacttcc gcagaccgtc ctgacgtgct ggttgccgcg tgcctggctg
     8221 ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc
     8281 gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca
     8341 ccaacacctg gcagacttct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg
     8401 atcacgagcg tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga
     8461 tcgattctct gactgccact tctgagcgca gccgtcgtct gtctccgcca gcgttccgtt
     8521 atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat cgttctgccg gaaggtgacg
     8581 aaccgcgtac cgttaaggca gccgctatct gtgctgaacg tggtatcgca acttgcgtac
     8641 tgctgggtaa tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg
     8701 caggcattga aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg
     8761 agctgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagataacg
     8821 tggttctcgg tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg
     8881 ttcacactac cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca
     8941 gctccctggt atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg
     9001 actgtgcgat caacccggat ccgaccgcag aacagctggc agaaatcgcg attcagtccg
     9061 cagattccgc tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg
     9121 ggacttctgg tgctggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg
     9181 aaaaacgtcc tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg
     9241 acgttgcgaa atccaaagca ccgaactctc cggttgcagg tcgcgctacc gtgttcatct
     9301 tcccggatct gaacaccggt aacaccacct acaaagcggt acagcgttct gctgacctga
     9361 tctctatcgg accgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg
     9421 cactggttga cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc
     9481 agtaatctcg tcatcatccg cagctttacg ctgcggatat ctgaaccgga aatagtcact
     9541 atttccggtt ttttattctc ttaatttgca ttaatccttt ctgattatct tgcttaactg
     9601 cgctgcatca atgaattgcg ccatctcaat ttgcatactt accactttgt tttgtgcaag
     9661 ggaatatttg cgctatgtcc gcaatcactg aatctaaacc aacaagaaga tgggcaatgc
     9721 ccgatacgtt ggtgattatc ttttttgttg ccattttaac cagccttgcc acctgggtag
     9781 ttccggtggg catgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa
     9841 cacgcaaagt cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg
     9901 agtatcaccg cgtacagctg ttcacgacgg gcgatgaacg cccgggtctg atgaacttcc
     9961 cgtttgaagg gttaacctca ggatcgaaat acgggacagc cgttggcatc atcatgttta
    10021 tgctggtgat tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta
    10081 tcctggcgct tattcgccat acccgcggaa atgaaattct ctttattcct gcgctgttta
    10141 ttctgttttc acttggcggc gcaatatttg gtatgggaga agaggccgtc gcctttgcca
    10201 ttattatcgc accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga
    10261 cctatatcgc cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggttg
    10321 ttgctcaggg gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt
    10381 gggttatcgc caccctgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa
    10441 agaatcctct tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg
    10501 atgttgaaca acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg
    10561 taatggtctg ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca
    10621 gccagttctt caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg
    10681 gcatgacggt taataccatg gcttcatcct ttaccgaagg ggcgcgaatg atgatcgccc
    10741 ctgccctgct ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg
    10801 gtgatgccag cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg
    10861 ataacgcggt cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga
    10921 catctggttc tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg
    10981 tcggtgtaaa ccgtcaggtt accgttctgg cgttccagtt tggtgatggc ttcagccaca
    11041 tcatttaccc gacctcagct tcgttaatgg caacgctcgg tgtttgccgg gtggacttcc
    11101 gtaactggct gaaggtgggc gcgacactgc ttggactgct gtttattatg tccagcgtcg
    11161 tggtgatcgg cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc
    11221 tcttttttca ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc
    11281 acaacgccag cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt
    11341 ccggcgtcag ccagcaaact tcgctgactt catcttcctg tagggcgaag ggaccgtgag
    11401 agacgcagct gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc
    11461 cgtgctcggc aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg
    11521 attccagcag ttgctcatcg gcctggacta caccgcctgc ggtcgcatct aacatgccgg
    11581 gtaaaaagtc ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca
    11641 cgacgatgta agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt
    11701 gtgcaatgac ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac
    11761 gctgttccat tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt
    11821 gggtaaatta cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta
    11881 cactaagtac gtcattatcc agcatgccat aactcgccgg attaccgcct ttaggaatac
    11941 tcaccgagcc ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg
    12001 tatgaccgta caccagcaca tcgttctggt ttaaagcagg taggttttcc gggccaaaca
    12061 gatgaccatg cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg
    12121 ttatcgggaa atgcaaaagc atttgatcca cttcgctgtc gcaattgccg cgcacagcga
    12181 taaccttatg tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg
    12241 gtaaagcatt acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac
    12301 cactttgggc aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg
    12361 atgcaaacat cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga
    12421 ttctgtcgtt gctatcagcc agattgcttc gccgaaagag caataaaacg ctggacagac
    12481 gctcgctgcc actggaatgt tgcataatcc accaggcgtt ctggcacctc atcgccatgt
    12541 agtaccaggc ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat
    12601 aaattcggct gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg
    12661 gcttttccct ccgccgttaa tggcgctttt ttcgcacccg caaaaacgac atctgtcgaa
    12721 cgttcttcgc ggatgggcat cagatcgcta cgcagccagg cctgaatctg tcgtgcacgc
    12781 gcacgatttt ctaaatcaag cggataaatg cgttcccagg tcggtggcgc aaatcgatct
    12841 tccagatact ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac
    12901 ggcacacggc gtgtctgacc gtaaccttgc cacgtcggct gcaaatgttc accgctgtcg
    12961 aggtcgatgg tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat
    13021 aacacataag gggagaaaaa atgggcatct gaccaaagcg tgatagcggg tttactcatt
    13081 atgacctcgg caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac
    13141 gtgacgaata ctcacgcaag tcgccgcgct ttctatactt gtggtgttca gatgattaga
    13201 caaccggagc tgcaatgatc gatctctatt tcgccccgac accaaatggt cacaaaatta
    13261 cgctgtttct cgaagaagca gggctggatt atcgcttgat aaaggtagac ctggggaaag
    13321 gcggtcagtt tcgcccggaa tttttgcaca tttcgcctaa caacaaaatt ccggcaattg
    13381 ttgatcattc tccagccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt
    13441 tgttgtatct ggctgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg
    13501 ccacattaca gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc
    13561 atcattttaa tcacgcagcc tcccaaacca ttccttacgc tattgaacgt tatcaggttg
    13621 aaactcagcg tctgtaccat gtactgaaca agcggctgga aaactcgccc tggctgggtg
    13681 gcgagaacta cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc
    13741 agcgaattga cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc
    13801 gccctgccac cggtcaggca ctgttaaaag cacaactcgg tgatgagcgt tcggatagtt
    13861 aacagaaaca ggttctcgtg tattatttca tcctaagaaa aacaacggag aacctgcaat
    13921 ggcacaacct gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat
    13981 taaggaagaa gaaattaaca accgtcagga tattgttatc aatgtgacga ttcactaccc
    14041 cgccgataaa gcgcgcacca gcgaagatat caacgatgcg ctgaattatc gcaccgtgac
    14101 gaaaaacatt attcagcatg tagagaataa ccgtttctct ttgctggaaa aattaactca
    14161 ggatgtgctc gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga
    14221 taaactgcac gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta
    14281 accgccatat cgggaggctg catgaagata gtgatcaccg gagggacagg attaattggt
    14341 cgccatttga ttccacgttt gctggagctg ggccatcaaa tcacggtaat gacgcgtaac
    14401 ccgcagaaag ccagttctgt tctcggccct cgggtggcac tatggcaagg gcttgccgat
    14461 caaagcaacc tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat
    14521 aaacgctgga ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa
    14581 aaactggtcg atttgattaa tgccagcgac acgccgccgt cggtactcat ttccggctcg
    14641 gcaacgggct attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat
    14701 aacgaattta ctcataaact ctgcgcccac tgggaagaaa ttgcctgccg ggcgcaaagt
    14761 gacaaaacgc gagtgtgcct gctgcgtacc ggcgtagtgc tggcaccgga tggcggtatt
    14821 ctcggtaaaa tgctgccgcc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg
    14881 cagtatctgg cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat
    14941 aacgagctgc gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt
    15001 gcccatgcgc tcggtcatgc tctgcatcgc ccggctattt tgcgcgtccc cgcaaccgcc
    15061 attcggctgt taatgggcga atcttcagta ctggtattag gtggacaacg cgcgctgcct
    15121 aaacggctgg aagaagcggg ttttgcgttt cgttggtacg atttagaaga ggcgctggcg
    15181 gatgtcgttc gctgatgtgg tttactgcaa acatccgcca gttaactccc ggtgttacag
    15241 ggttagtggc tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac
    15301 tctcggtcta aacctctctc cagcatctcc tgagcaatac gcagggcttc ttcgcgtttg
    15361 ccaagaatta atccatcatt ccgcccttct tcacgtaatc tgtcagcaat agtcatcagt
    15421 ttctcctttt cttgtggtgc gcgttccgct atctcaccaa taaatgcacg gaaacgctgg
    15481 gcatcccccg tttgtaatac gtaattaaac agggctttta gctgtctgtc attagtgttc
    15541 cctgtaacta gcagcgaaac aatttggtcg actaatccca acagatcgcg ctgacgaata
    15601 tgtttctgaa ttaactccaa cagcgccatt ttgcgatgtt gcataatctc gtcatccggc
    15661 accacggtaa tatctaccag cggaaaagcc gatgaataaa ttttgcgggc tatagcaggc
    15721 tcggcaaatt catcaagcca gcaaagtgaa taaggataag gacttctgca accatgataa
    15781 aacagcatcg ggatcaccaa tggaagctct ttatagcctg catcaagatg gttttgcatc
    15841 gccgcaatgg aatagcgcat catgcgaaaa gccattaatt cttccggctt actttggtgc
    15901 tctatcacta cataaatata acctgctccc tcctgcgttt tcacagacca caagaggtcg
    15961 gaataatatt gccgcaggtc ttcatcaata aaactgtttg gttccagttt tagcgttgtt
    16021 aaatcacaca gtttgcgcag cggcgcggga agatgaatat caataaaatc ccgcgcggtg
    16081 tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg gcgtggaagt tgtcgatatt
    16141 gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg acaataacca cgattaatac
    16201 ccaatgcagc aggcagtttt tatctttgga cagcgaattc agaactattt ttagcggact
    16261 gcaactttta catttcagcg gaagctgact cgcaaattcc ccttgaaaat actccggcag
    16321 cgttagccac cggaggggga attatttcag cgatccctta aggaactgtt gcagacgagg
    16381 gctttgcggg ttaccaaata actgttctgg cgcgccctct tcttctattt tcccctgatg
    16441 gaggaaaatg acgtgagtag aaacatggcg agcaaagccc atttcgtgag tcactaccac
    16501 catggttttc ccctcttctg ccagttgctg cataatacgc aacacttcgc ctaccagttc
    16561 aggatcgagc gccgaggtag gttcatcaaa cagtaaaact tccggttcca tcgccagcgc
    16621 ccgcgcgata gaaacacgct gttgctgacc accggacagg tgcaccgggt atttcccctg
    16681 cgcacgttcg tctatcccga cttttgccag atatttcacc gcccgctcgc gcgcttcctg
    16741 cttgctcagg cccaacacct gaatcggcgc ttccatgacg ttttccagca ccgtcatatg
    16801 gctccagaga ttgaagtgct ggaataccat cgtcaggcgt gtgcgcagta agcgcagctg
    16861 atttttatcg gcgactttga gttgaccatc tttgtcgcgc accagattga tcgtctggcc
    16921 attgaccacg atcgatcctt cgctcggttt ttcgaggaag ttaatgcagc gcagaaaggt
    16981 acttttcccc gatcccgacg atccgatgat gctaattaca tctccggcat tcgcttgcag
    17041 tgaaacccct ttcagcactt cgtgttcgcc gtagcgttta tgcaaatcga taacgtttaa
    17101 tttattctcg gacatcgtat tctcagtgcg ttgaagaagg tttcacatgc tgcaaccagc
    17161 gtttttccgc tctgcgaaag aggctgatca ggacataaga gatgattaaa tagagcaccg
    17221 cggcaatgcc gaaggcggta aaaggttgat atgtggcggc gttaatatcg cgggcaattt
    17281 tcagcagatc cggcaccgtg gcagtaaatg ccagcgcagt agagtgcagc atcaggatca
    17341 cttcgttgct gtacgccggt aacgcaatac gcagcgcaga aggcaaaata atgcagcgat
    17401 acattttaaa ggtcgagaag ccataggctc tggcggcttc aatttcccca tgcggcactg
    17461 aacggattgc cccggcaaaa atctcggtag tgtaagcgca ggtgttaagc gtcagcgcca
    17521 gcacggtaca gttcagacca ctgcgaaaaa aggcgttaag gaactcggtt cccttaacaa
    17581 tctccagcgt gtacatgccg gaatagaaca ccagcaactg aacatacagc ggcgtaccgc
    17641 gaaaaatata ggtaaataac cagattggaa actggatgta tttattactt gagacgcgac
    17701 caatcgccag aaacagcgcc agaactccgc ctatcactac cgacaaaata agcagccaca
    17761 gggtgatcgc cacgccagta aagcgataac cgtcggtcca cagcagtggt ttccagtatt
    17821 catgtaagat ttcgatcaca ggtcagccct cttcacaccc acggagtagc ggcgctcaag
    17881 gaacagcagc acaccattgg aaacagtggt gaaaaccagg taaatcacgc cacagacgat
    17941 ggcgaaatag aacggttccc aggtactttt gcctgccagc tgagtggctt tgaccacatc
    18001 ttccaggccg agtaacgaaa cgagtgcggt agatttgagg atcacctgcc agttgttgcc
    18061 aatgcctggc agggcgtaac gcatcatcgc cggaaacatg atccgtcgaa acacttgccc
    18121 acgagtaaaa ccgaacgccg tcgccgcctc tatgtgtcct ttcggcactg ccataaacgc
    18181 gccacgaaac gtttcggtaa aataagcacc gtagataaaa ccgagagtga taatacctgc
    18241 gaccatcgga tcaatatcaa tctgcccgac gcccatcgcc tccgtcaccg tgtttagcgc
    18301 aatctgcaaa ccgtagaaaa tcagcagcat caacaccaag tccggcacgc ctcgaatcag
    18361 cgtggtgtac ccttcgaaaa tcagccccga aagccgattt tgcgagagct taccgccagc
    18421 gccaattaaa ccgataatta cagcgagcac tacagagctg atagccagct ccagcgtgac
    18481 gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac atgctgcctg tcctgtcgtg
    18541 tgtggtgaat taccgcctct tgtctccctc cggttacccg aagggagcgg actttctttc
    18601 accgatggcg attaaccacc ataaacatca aaatcgaagt actttttcgc taatttctcg
    18661 taagtaccgt cagcgcgcat ttcggcaaag gctttgttca gtgcctcgcg cagttcgtta
    18721 tcttctttac gcaggcccat gccggtccct acgccaaaca gtttttcatc tttaacagac
    18781 ggaccaccga atttgtaatc tttaccgacg ggttgtttga ggaaaccttc gctggcagcg
    18841 acctcatcct ggaacgcggc atcaatacgt ccggcagtca ggtcagaata aatgttgtcc
    18901 tgcccctgat aagaaacgat ttcaatgcct tttggtgccc aatgttcgtt accgaacgtc
    18961 tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt tcagcgactc gactgtcggc
    19021 tgaatgtcgg aatttttcgc caccaccaga cgagaatcgg cagcgtacag tttgtcggtg
    19081 aaggcaattt cttgctgacg tttttccgta atggaaagcg atgacatgat ggcgtcaatc
    19141 ttcttcgctt ttaaggacgg gattaacgca tccagtgggt tttcgacaaa cgtacattgc
    19201 gtattgatgc gtttgcacaa ctccttcgcc agatcgatat cgaagccaac cagttcgcct
    19261 tgtgaatttt ttgattcaaa tggcgcatag gtcggatcgg taccgatgcg gatgttttgc
    19321 ggaatcgcag caaacgccgc agttgcgctg gagaaggcca gaaccagaga gagcgatagc
    19381 accagttttt tcataatgtc ctcaactgac agtcttttta tagggaatat ttacaggttt
    19441 gttacactta tcgtgccata aaattgacca tcaaggcaaa atatcccgcc ccgttagacg
    19501 tattattttt cattaaaaat gcttaagtat gcatttcaat gcaccacgat agtgcaacaa
    19561 acctggcgtg caccatgatg atcattccat caggtacagt ttccctgcga cgtatcagtc
    19621 accgtagaca ttaaagtcga aatacttttt cgccatcttg tcgtaggtac cgtcctgacg
    19681 cagctcgcca agcgccttat taaacgccgc cgtcagttca gcatcatctt tacgtagccc
    19741 tacaccggta ccgtcaccga agtatttttt gtctttcact gatgagccag caaaggcgaa
    19801 atctttacca gcaggttgct tgaggaaacc ttcgctggcc gccacttcat cctgcagtgc
    19861 ggcatccaga cgtcctgcag ccagatcgga atagaccaaa tcctggttgg cataggccac
    19921 cacatccaca cctttgctac gccaggtctc gttagcgtaa gcttcctggg ttgatccctg
    19981 cagcacaccc acatgtttac ctttcagtga atccagcgtt ggctgaatcg gcgaaccttt
    20041 ggcggcaatc aaacgagaat ctgcggcgta caacttgtcg gagaaagcaa tctcctgctg
    20101 acgtttatcg gtaatggaga gcgacgaaat aatggcgtcg atttttttcg ctttcagtga
    20161 ggggatcagc gcgtcaaagt cactggcaac ccaggtacat ttcacctgca tccgtttgca
    20221 catctcgtta ccgagatcga tatcaaagcc aacaaagtca cctttggcat ctttcgatga
    20281 gaacggcgca taggtggtat cggttccgat acgtaccgtc tccggtagcg ccgcatagct
    20341 ggaagccact gcggagagac cgactaacaa agacagagcg agaatcgact tcttcataca
    20401 taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg tgttgtgtgt ttgcaggctc
    20461 tttcatgcag gtcttatgcc atctttacga caacagtaac attcaacgtt aaatatgtta
    20521 ataagacgtt gcattattgt cctaaagttg aagatagcag gtatggcggt tgaatcgcag
    20581 cgtttcgatt catcgaaagg aacaaaatgt cgaggatttg atcgcggttg caaaattgcc
    20641 ctgaaacagg gcaacaatga ggttatgcgc cctgccagcg ggcaaagaga tcttcaggaa
    20701 gggttatcgc aaactggtca agaacacgat taaccgtctg atttatcaca tcatcaaggg
    20761 attgtgggcg atgataaaac gccggaacgg gaggcataat caccgcaccg atttctgccg
    20821 cctgagtcat taaacgcaga tggcctaagt gcagtggcgt ttcacgcacg cagagcacca
    20881 acgggcgacg ctctttcagc accacatctg ccgcacgggt cagtaagcca tcagtatagc
    20941 tatggacaat gccggacagg gttttgattg aacagggtaa aatcaccatc cccaacgtct
    21001 ggaaagaacc ggaagaaatg ctggcagcaa tatcgcgcgc atcgtgtgtc acatcagcta
    21061 acgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga taaggtctgg cgcgctgcct
    21121 ggctcattac cagatgcgtt tcgatatctg tgacatcgcg cagaacctgt aataaacgca
    21181 cgccataaat cgcgccgctg gcaccgctga tgcctacaat gagtcgtttc ataaatagtt
    21241 gccctgtcag acttgcaggc agactttgca ggatttcgac gggagttgca agtcagggtg
    21301 ccagaccggc accctgagcg aaggcatcat ccttcgttat gcatttcgag attttccact
    21361 tcgttctgac gttgcactgc tttggcgtcg tcattacgta aagtatcgag gaaatcgagg
    21421 tagccctgat caacgtcttt ggtgacgtag acgccgttga ataccgagca ttcaaactgc
    21481 tggatatccg ggttttcagc gcgaacggct tcgatcagat cgttcagatc ctggaaaatc
    21541 aacccgtcag caccgatgat ctggcgaatt tcatctactt cgcgaccgtg agcgatcagt
    21601 tccgtggcgc tcggcatatc gataccgtaa acgttcggga agcgaatttc cggtgccgca
    21661 gaagcgaggt acactttctt cgctccggct tcgcgtgcca tctcgataat ctgctcagaa
    21721 gtggtgccac ggacaatgga gtcgtcgacc agcaagacgt ttttatcgcg gaactcggcg
    21781 cggttggcgt tcagtttacg gcgcacggac ttacgacgca gctgctggcc cggcatgatg
    21841 aaggtgcggc caacatagcg gtttttaacg aagccctggc ggtacggttt gcccagaata
    21901 cgagcaattt ccagcgcgat atcacacgag gtttccggaa tagggatcac cacgtcgata
    21961 tccagatctt cccattcgcg ggcaattttc tcgcccagtt tggtgcccat attcacgcgc
    22021 gcgctgtaaa cggaaatttt gtcgatgaac gagtccgggc gggcaaagta tacatactca
    22081 aacaggcacg gattgctgac cggattgtca gcacattgac gggtaaacaa ctgcccttct
    22141 tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca ggaaatcaaa gcccagcgta
    22201 tcgagcgcaa cgctttcgga agcgaccata tattctgtac ggttctcgtc aatatcacgt
    22261 tttcccagta ccaacggacg aataccgttt ggatcgcgga aagcaaccat accgtggccg
    22321 atgatcatcg ccacacaggc atacgcgccg cggattaagc ggtttgtagc agcgatggcg
    22381 gcgaaaatgt tgtcggcttc cagcggatag tggcggaagt tgtccagttc gctggcaaag
    22441 atattaagca gaatttccga gtcggaagtg gtgttgatgt ggcggcgttt ttcttcaaac
    22501 agttttttac gcaactcgtg agcgttggtc agattgccgt tgtgggcaag cgtaatgcca
    22561 tacggggagt taacgtaaaa cggctgcgct tcagaggcgc tggagctgcc agccgtaggg
    22621 taacgcacat gaccaatgcc catattgccc tgcaaacgct gcatatggcg agcttcaaat
    22681 acatcgctca ccagcccgtt cgctttacgc aaacggaagc agttattggc atctatggtg
    22741 atgatgccgg cggcatcctg accgcgatgc tgaagcaccg ttaaggcatc ataaatcgac
    22801 tggttaaccg gcataacacc ggcgataccg acaataccgc acatacgtct ttttcctcgt
    22861 taagccacat ctcagagcac ttacgctctg ggcaagaaac ttgacgagct ttgcagataa
    22921 tcaaaaaacc atctgatgat aaaactgaac tgcgggatca gctgtgattt gctccagtct
    22981 tcgcttttcg acaccccggt aaaggagtcg agaaagaaga gaatggcggc aacaatcaac
    23041 acaccgcgca acgcaccgaa acagacgccc agcacccgat cggtgcctga caaccccgtt
    23101 ttctctacca actggcctat cacgaagttc acgatagcac caacgatcag ggtagcgata
    23161 aacagtaccg cgatggcaat cccatttcga accagttcgt cttcaaagcc cgtaaaccag
    23221 actgacaggt aagtgtagta atgactggca acaaagaaag cacaacccca tgtcaccagc
    23281 gataacgctt cacgaacaaa gccgcggatc aggctaacca gagaggaaaa agcaatcacc
    23341 gcgattatgg cgtaatcaat ccagaccata tgtgtcccac gattttacgc cctgtcatcc
    23401 tgttcggggc gcattctaac agaaaaagaa aacgtttgcg tagggatttc cttcccgcgc
    23461 atcaataaaa atggcgctga aaaaacattc aacgccatcg actttttatg cctttgcggc
    23521 atcgggcaat gcgtgtcgga tgcggcgtga acaccttatc cgacctacgg ttctacccct
    23581 gtgtaggcct gataagacgc gccagcgtcg catcaggcaa gaccgtatta attcggcgta
    23641 tagcccatta ccacgccact taagccagaa agttgcttca actcacccag cgatcctttc
    23701 agcttatctt tcgaggcatc aggcccaacc agaatacggg taattttacc ctgcactggc
    23761 gtggatggcg acgtataaac ccgataaccg gcaccgcgca gcttaccgac aatctcattc
    23821 actttatcgg catttttcag cgcgcccagt tgcacaacat aggctttacc cgttggcgct
    23881 gctttttctt ccacgaccgg cttcgacgca ggtggtgctt caaccttcgg tttaggcgac
    23941 tccaccggtt tcggcttcgg tggggcgacc ggtgcaggtt ccggttcaaa ctcggtgtta
    24001 ttgactgcaa tagtggccgg atcgagcgac ggtgctgcgg catcacctgc ccgcacctct
    24061 tccgctgcgc cttccggcgg ctgcgtcggc agcgcctggg tggcggctgg catcatatca
    24121 ggctcatcgc gatcgcccgc tttcggcacc agcgggatag ccgcgaactc atcctgataa
    24181 tgttttttct gcccgtccag cagccctgga agtacaatca cccccagcgc caccagcacg
    24241 atcgtgccca ctaaccgatt ctgaaactta cttgccaccg cttctcctcg cgtcaatcac
    24301 ttccatgaca tgtgcgaccg tgtggaacga accacacacc agcacggtat cttccgcttt
    24361 agcgtccgcc attgcggcat cccatgcctg cgccacgcta tcaaatgatt tgccgttacc
    24421 caaatgctca agcagttgtt ctgccgtggc accgcgcggc ccttccagtg gcgcacaata
    24481 ccagtcatca accacgcttt tcaaccaggc cagagttccg gcaatatctt tatcatgtag
    24541 cataccgata accgccagca cgcgcccgtt tttcggtagc gctttcatac gcccggtgag
    24601 atattccgcc gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg actcgctcac
    24661 aatctggaaa cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg cattttcact
    24721 gacctccagc ccgctggcac gcagtgccgc cagcgccgtt gcggcattcg gttgcgggac
    24781 aagcggcaac ggcagatttt ccagcgtgcc gtgagcatcg ctaaacgccc agtcatgatc
    24841 ggtgacggaa tagttccact caacgccccg acgttgtaac agtgcacttt tttcctgcgc
    24901 cacatcagca atggtagaag gcatttccgg ctcaccgaca attgccggtt tttcgctgcg
    24961 gaagatgcct gctttctcgc gaccaatgct ttcgcgatct ggccccagcc agtcggtatg
    25021 atccagcgca atgctggtta ctaccgcaac atcggcatcg acaatattgg ttgcgtccag
    25081 acgaccgccc agccctactt ccagaatcac cacgtcaagt tgtgcctgtt tgaacagcca
    25141 caacgccgac agcgtaccgt actcgaaata ggtcagggaa atatcaccgc gtgccgattc
    25201 aatctccgca aaagaggcgg tgtgggccga ttccggcaat tcctggccct gcacacgtac
    25261 gcgctcggta taacgcacca gatgcggcga agtgtagacg cccactttgt accctgccgc
    25321 catcagaatc gactccagcg tacggcaagt ggtgcctttg ccattcgtac ccgcaacggt
    25381 aaacacaaat ggcgcaggtt tcaggacgcc aagacgcgcc gcgacctggc tcacgcgctc
    25441 aaggccgaga tcgatagttt tactgtgcag gttttccaga taagaaagcc acgaagccag
    25501 aggcgacgcg gcttgaggag tgcgtttgat aatcatggta tccgctgatt cgttacggtg
    25561 agaatagcaa aagggcagag ccagtggccc tgcccttatc agttatcagg cctcaggttc
    25621 ctgatctggt accggtggca ccactacgcc ttcacgcggc gcttcaggat tcggcgctgg
    25681 cagattcatc aacttcgcca gaatgctcgc cagtttcagg cgcatttccg gacgacggac
    25741 gatcatgtcg atcgcgcctt tctcgatcag gaattcactg cgctggaatc ctggcggcag
    25801 tttttcgcga acggtctgtt cgataacacg cggaccggca aagccgatta acgctttcgg
    25861 ttcagcgatg ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc ccatggtcgg
    25921 gtcggtcagc acggagatgt acggcaagcc gcgctcctgc atttttgcca gcgccgcaga
    25981 ggttttcgcc atctgcatca gcgacatcag tgcttcctgc atacgtgcgc caccagaggc
    26041 ggagaagcag atcagtgggc agttatcttc cagcgcctgc tcaacggcac gcacgaaacg
    26101 tgcacctacc acagacccca tggaaccgcc cataaaggcg aactcgaatg ccgcagcgac
    26161 aactggcatt ccatacaggg tgcctttcat caccaccagc gcatcttttt cgccggtttc
    26221 tttctgcgca gatgccagac ggtctttata cttcttggag tcacgaaact tcagcacatc
    26281 tttcggctca agctcgctac ccagctccac aaggcttcct tcatctaaca ggctatgcag
    26341 gcgattacgc gctgtcatac gcatgtgatg gtcacacttc ggacagacct caagattacg
    26401 ttccagctca gcgcggtata aaacctgacc gcagctatca cacttagtcc acaccccttc
    26461 aggaatgctc gccttgcggg tgggagtaat gttgctttta attcgttcaa tccagctcat
    26521 tagggacctt tctgtctgaa cctggttcga tgccagtttt atctttgggg acgcataatg
    26581 ccatttttgc ccccaacaga ccatgaatgt tgcacattaa aacataacag cccgaaactt
    26641 tggataaaaa agtggtcgaa ccgcggagtt actttttatt ttgcggcgcg tgccgcagcg
    26701 cgtttgtggc gaataaattc gaaaacacct ggcaatattg aaacaaaaat aatgccaaca
    26761 atcatcagtt taaggttgct ctgaatgaag gggagcgttc cgaagaaata acccgcgtag
    26821 gtaaaaatca gtacccatag caacgcgccg atgacgttaa aagcggcgaa atgacgatac
    26881 gacatgtggc ccattcccgc aacgaacggc gcgaacgttc tgacgatcgg cacaaaacgg
    26941 gcgagaataa tcgttttgcc gccatgtttc tcataaaact gatgagtctt atcaagataa
    27001 ctgcgacgaa agattttcga atccggatta ctgaacagtt tgtcaccaaa taaccgtcca
    27061 atggtgtagt tcaccgcgtc acccacaatc gcggcgatca gcatcagcac caccatcata
    27121 tggacgttga gatcgttggt ttccagtgat gccaacgcac cggcaacaaa cagcaatgaa
    27181 tcacccggta aaaacggcgt taccaccaga ccggtttcac agaacaaaat taagaacaaa
    27241 atggcataaa cccagacgcc atactccgcg accagttctg ccagatgcac gtcaatgtgc
    27301 aggataaaat cgatgaggaa ataaatcagg tccatattag cctatgcctt gtactcgtca
    27361 tctttcaggc tgtaactacg ttggctacag cctgaaatgt tccgagcatt attcttgtta
    27421 gtccgccaga aatagcgggc ccattggcgg ttttggaaga tcataccggt cagggtaatc
    27481 caccgcgacc agatacagcc cttccgcttt tgccgttgct gccgccagcg ttctgtcctt
    27541 tgccgccagc aactctgcta tccagctctc cggctggttg tgggcaccga cttccatcag
    27601 gctgccgaca atattcctga ccatatgatg tacaaaggca ttcgctttga tatctaccac
    27661 cacataagga ccgtgacgcg tgacattaat atgcataacg ttgcgccacg gggttcggga
    27721 ctggcactgc accgcacgga acgaggtgaa atcattctcg cccagcaagc attgcgcagc
    27781 ccgatgcatc cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt tactcagtac
    27841 cgccgggcgc agccgatgat tgtagatgat gtagcgataa cggcgagccg tggcgctaaa
    27901 tcgggcatga aaatcatcag gtacagcttt aacccaacgc acggcgatgt caccaggtaa
    27961 attcgcattt acgcccaacg tccacgcagc gtctttgcgc tgtgcggtcg tttcgaaatg
    28021 cacaacctgc ccggtaccgt gtacaccggc gtcagtacgc ccggcgcaga agacggtgat
    28081 gggttcgttc gccacctggg agagcgcctt ttccagcttc tcctgcacac tgcggacttc
    28141 attctgccgt tgccagccgt aatacctact gccgtcgtac tcaatgccca gcgcaatttt
    28201 ataaactggc ggttgttgct ggtcggacat tagtacagat actcctgcac cagtttctcg
    28261 gcgattttta ctgccatcag cgcgccgcca aagcgaacgt tatcggccac cgaccagaac
    28321 tgaacttgct ccggcatacc gtagtcatta cgtacgcagc caacagaaag atgcggcgta
    28381 ccagaagcat ctcctacctg agtcgggaat tcgttctctt cagagagcac aatatcttcg
    28441 ccctgagcaa acgcatcacg cgcttcttct gccgccagcg gacgcagtgc ttcaaagttg
    28501 accatctggg catgaccgta gaataccggt gcctggacga cgcttgccga aatcatcagc
    28561 ccttcgtcct gcaggatttt gcgtacttcg tcaacgatac gacgttcttc acgcacgcta
    28621 ccttcgctat ccggcagtaa cggcagcatg ttgaacgcca gctgacgccc gaagaaatct
    28681 tcttcgtcaa tcggaatgcc attgagtaat ttagcactct gccccgctaa cgcatcgacc
    28741 gcttttttgc cctgggcgga ggctgaaatc aggctggtaa cgctgatacg tgataaaccg
    28801 ccctgatcaa ttagcggttt cagtgccgcc agcagctggc tggtcagact gtccggtacg
    28861 gcgatgacat tccggttgcg gtaatcggtc agtacaaacg ggtttacttc cggcactacc
    28921 agcggtacgt caggttcgag agcaaacaat ccgctgctgt cgatcaccag acaacctgag
    28981 ttggtcgctt cttcaaccca ggcagcggta gcttctttgc ctgcgacaaa aaatgccagc
    29041 tgcgcctgcg tccagtcaaa ttcagcggca tcctgcacgg tgattgtctt accaccaaag
    29101 cgcagttgtt cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc aaccgggaac
    29161 tgacgttcag ccagcgtttc aagcagggct tcgcccacag cgccagttgc gcccaggacg
    29221 gcaatgttcc agccttcaga catggtggtt tactccagaa atagcaaagc tccctgccaa
    29281 tgttacagca gagagcatga agaagagatt aacgtgccgg atgatgaacg gcgttaaaac
    29341 ccagtttaca cagcaatgat gccgcactgg cgtcatcaca aattacatac agagatgacc
    29401 attcacggcg ctcaagatag tttttgcgca gtttatcgaa ctcacccggt atcccggcga
    29461 ctttacgcag tggcgcatca tcgcggcgca catcatacac caaatgcacc agccttttca
    29521 gcgtcggttg atcgagcgga ccatgcagcg taatgcgacc aaactctggc gcgggcagta
    29581 atgtatccag cgcaacgtgt tgttcatgcc caatgaattt gctataggct tcaaacactt
    29641 gcgtagtacc gcgtgcttta ccttccaggg tatagcctgc aatatgcggc gtgccgatat
    29701 ccacttttgt cagcaattcc acattaagtt ccggctcgcc ttcccagaca tccagcacca
    29761 cgcttaactt ctggccttca ttcaggcagg tcagcagcgc ggtattatcg acgactgcgc
    29821 cacggcaggc gttaatcaga atcgctccgg gcttcaggct acggatcagt ttttcatccg
    29881 ccagatgtcg cgttttgtac ggaccatctt taaagagtgg cgtatggaaa gtcagaatat
    29941 ccgcatgctg gactaattca tccagcgagc ggaaatcgcc ctcatcccca cggtcggcgc
    30001 gaggcggatc gcaaagcaag gttgtaatcc ctaacgcttc cagtcgtgcc tgtaaacgcc
    30061 gaccaacgtt accaacgccc acgatcccca cggtacgctc gtgcagtgaa aatccatcgc
    30121 gttcagcaag catcagcagt gaggagaaaa catattccac tactgcaatc gcattacagc
    30181 caggtgcagc ggaaaaacca attcccgcct gctttaacca tgtgtcatca acatggtctg
    30241 tccccgcggt ggcagtgcca acaaatttaa tgggttttcc tgccagcaaa gattcattca
    30301 ctttcgtgac cgaacgcacc atcagcgcat ccgcgtctgc cagttgagcc acggggatgg
    30361 gacgcccggg aaccgcggtc acctcaccca aacggctaaa taagtcgcgg gcataaggca
    30421 tattttcatc aacaaggatt ttcacgtttg tgttacctgt atgagacgag agttaaccgg
    30481 acaagtgtgc cataatctcg cggccaggca tacttgcgaa gatttcaggt ataaggatac
    30541 gtaatgatac aacctatttc cggccctcct cctgggcaac caccaggtca gggagataac
    30601 ctgccgtctg gcgcgggcaa tcagccttta tccagtcagc aacgtacttc gctggaaagc
    30661 ttaatgacga aagtgacctc actgacgcaa cagcaaagag cagaactgtg ggcgggtatc
    30721 aggcacgata ttggtctgtc gggagattca ccgctgcttt cgcgtcactt ccctgccgct
    30781 gagcacaatc tggcgcaacg tctgctggcc gcgcaaaaaa gccattctgc ccgccagctt
    30841 ttagcgcaat taggggagta tttacgtctg gggaataatc gtcaggcggt cacggattat
    30901 atccgtcata actttggtca gacgccgctg aatcagctct caccggagca attaaaaacc
    30961 attctaaccc tgttgcagga agggaagatg gttattccgc aaccgcagca gcgcgaggcg
    31021 accgaccgtc cgttattacc ggcggagcac aatgcgctca aacagctggt gaccaaactt
    31081 gcggcggcaa cgggagaacc cagcaaacag atctggcaat cgatgctgga actttccggg
    31141 gtgaaagatg gcgagttaat tccagcgaaa ctgtttaacc atctggtgac ctggctgcag
    31201 gcgcgtcaga cgctaagcca gcaaaatacg ccgacgcttg aatcactaca gatggcgctg
    31261 aaacaacctt tagatgccag tgaactggcg gcgttatcgg catatatcca gcaaaaatat
    31321 ggcctttctg cgcaatcatc gctttcttct gcccaggccg aggatattct taatcagctt
    31381 tatcaacggc gggttaaagg gattgatccg cgtgatatgc aaccgctgct taatcctttt
    31441 ccaccgatga tggacacgtt gcaaaatatg gcaacgcgtc ccgcgctgtg gatactgtta
    31501 gtcgcgatta tcctgatgct ggtctggctg gttcgttaac cccgacgaaa tgacagtatc
    31561 gtgacaataa ttcccagcac cgcagagatc gccccggcaa gaaataccga agagtaacca
    31621 aacgtggtcg ccagcattcc cgcaagcggc ccggagacgc cgagggcgat atcctgaaac
    31681 gcggcgtaac cgcccagtgc ggtgccgcga acgtgtgacg gtacgcgttt aaccacctcc
    31741 acgcccagcg caggaaagat aagcgaacat ccggctccgg ttaacgccgc gcccgctaat
    31801 gcgacccatg cacctggggc ttgccagagc agcaacaagc ccaccgtttc tacaagcaga
    31861 gagacgatcg ccactttcac gccgccaaaa cggtccggca tccagccaaa catgacgcgc
    31921 atcacgacaa atgcgccgcc aaacgcggta agcgtaaagc ccgccatcgc ccatcctttg
    31981 ctggcaaagt agagcgaaac gaaagtcccg ataaccgcaa aaccaacgcc ttgtagtgcc
    32041 agacctaacc ctggtttcca gataagcccg acaacgctcc acagcgatgg acgttccccc
    32101 gccagggccg gtactttgcg cactgtgccg ttacaggccc acgccagtaa gggtaatgcc
    32161 attgtggtga gcgccagtgc ggcaaaaccg taatggctat gaatcaatag gccaagcggc
    32221 gcaccaacag cgagggcacc gtaaatcgcc attccgttcc atgacatcac tttgccggaa
    32281 tgttttggcc caacgatgcc taatccccag gtcagagcgc ccgtcagtaa ctggctttca
    32341 ccaaagccaa gaatcaaacg cccgacgacc aacagggcaa atttgaacgg tgcggggacg
    32401 ggcaaaatcg ccgccagcag caacgcgccc cccgccagac cacaagctaa catcccctga
    32461 agcgccgaac gttttgcgcc atattgatcg gccagtcgcc cggcgtaacc acgcgtcagc
    32521 accgtagcca gaaactgaat cccgacggca atgccgacca tagtgttgcc atagcccagt
    32581 tcatgatgaa caaacagcgg gataaccggc aacggcaacc ctacggtcat gtaggtgaga
    32641 aaaaccgcaa aagcgatgcg gaagagcgaa aaattggcag aagatcgtgt ttcggtttgg
    32701 cttacagcag tcatgcatta ctccagaatg cagcgcaagg cgaggagtat ccccgtctca
    32761 tctctctggt ttcagggtta cggtgcgttg gcaggaatta acgcgtacgt cttttcagaa
    32821 ggaaatcgac aaagcgggaa gtttgcctgg tacaggcggc gattgtcaat gatgtgaaaa
    32881 agggaaccat agggttccct tattctttag taccggaggc agcattgccg catatcgtcg
    32941 gatgcgacgc tgacgcgtcc tatccgacct actccgaatt aatctttcag cttgcgcatt
    33001 accagcgtgg cgttggtgcc gccgaagccg aagctgttag acataacggt ggtcagttcg
    33061 cgatcggtcg tttcggtcac gatgttcaga cccgcagcct gctcgtccag ctcttcaatg
    33121 ttgatgctcg gggcgataaa gccgtgttcc agcatcagca gagagtagat agcttcctgt
    33181 acgccagcag cgcccagaga gtgaccggtc attgctttgg ttgcagaaat cgccgggctc
    33241 ttatcgccga acacttcacg gatagcagcc agctctttca cgtcgccaac cggagtcgaa
    33301 gtaccgtggg agttcaggta gtcgattggg gtatcaacac cgtgcatcgc catcttcatg
    33361 caacgcactg cgccttcgcc agacggagca accatgtctg cgccatcaga cgttgcgccg
    33421 tagccaacga tttcagcgta gatatgagca ccacgtgcca acgcgtgttc cagttcttcg
    33481 accaccacca taccgccgcc gccagcgata acgaaaccgt cacggtgagc gtcataagta
    33541 cgggaggctt tttccggggt gtcgttgtat ttggtagaca gcgcacccat cgcgtcgaat
    33601 tcacaagcca tttcccagca cagctcttcg ccgccaccag caaacacgat gtcctgtttg
    33661 cccagttgga tctgctctac tgcgttaccg atacagtgtg cggaggtcgc acacgcggag
    33721 ctgatggagt agtttacacc gtgaatttta aacggcgtgg cgagacaggc agaaacgccg
    33781 gatgccatcg ctttggtgac cacatacggg ccaaccgctt tcaggccgcg cgggccgcgc
    33841 attgcgtcag cgccgaacac ctgaaaacgc ggggaaccgc cgccggaacc tgcaatcagg
    33901 ccaacgcgcg ggttattctg gtaagcttcc ggagagaggc cagcatctgc aattgcctgc
    33961 tccatagaaa ggaatgcata aatggatgcg tcgctcataa agcgcacaac tttgcggtca
    34021 atgaggccag tggtatccag ttttacgttg ccccagacgt ggctacgcat gccggaatcc
    34081 ttcagctcct gagagaaagt gatccctgaa cgtccttcac gcagagatgc caggacttcc
    34141 tgctggttat taccgatgct ggaaacaatg cccaggccag taatcactgc acgtttcatt
    34201 caatacctct gtaagtcgca catagagtaa gtttcgaatg cacaatagcg tacacttgta
    34261 cgccgaacaa gtccgatcag ccaattaata gagaaatttg cgcagcctga cacacatcgt
    34321 taagatcgtc agactgcccg tcagacgagt aacttacgtg aaacactacg ccatacaacc
    34381 tgccaacctc gaatttaatg ctgagggtac acctgtttcc cgagattttg acgacgttta
    34441 cttttccaat gacaacggac tggaagagac gcgttatgtt tttctcggag gtaaccaatt
    34501 agaggcacgc tttcctgagc atccacatcc tctgtttgtg gtagcagaga gcggcttcgg
    34561 caccggatta aacttcctga cactatggca ggcatttgat cagtttcgcg aagcacatcc
    34621 gcaagcgcaa ttacaacgct tacatttcat tagttttgag aaatttcccc tcacccgtgc
    34681 ggatttagcc ttagcgcatc tacactggcc ggaactggct ccgtgggcag aacaacttca
    34741 ggcgcagtgg ccaatgccct tgcccggttg ccatcgttta ttgctcgatg aaggccacgt
    34801 gacactggat ttatggtttg gcgatataaa cgaactgacc agccaactgg acgactcgct
    34861 gaatcaaaaa gtggacgcct ggtttctgga cggctttgcg ccagcgaaaa acccggatat
    34921 gtggacgcaa aatctgttta acgccatggc acgactggcg cgtccgggca gcacgctggc
    34981 gacatttacg tctgccggtt ttgtccgccg cggtttgcag gacgccggat tcacgatgca
    35041 aaaacgtaag ggctttgggc gcaaacggga aatgctttgt ggggtgatgg aacagacatt
    35101 acccctcccc tgctccacgc cgtggtttaa tcgtacgggc agcagcaaac gggaagtagc
    35161 gattatcggc ggtggtattg ccagcgcgtt gttgtcgctg gcgttattac ggcgcggctg
    35221 gcaagtaacg ctttattgcg cggatgaagc ccccgcgctg ggtgcttccg gcaatcgcca
    35281 gggggcgctg tatccgctct taagtaaaca cgatgaggcg ttgaaccgct ttttctctaa
    35341 tgcgtttact tttgctcgtc ggctttacga tttattgccc gtcaaatttg atcatgactg
    35401 gtgcggcgtc acgcagttag gctgggatga gaaaagccag cataaaatcg cacagatgct
    35461 gtcgatggat ttacccacag aactggctgt tgcggtcggg gcgaatgagg ttgaaggcgt
    35521 tacaggtgta gtaacaaatt gtggcggtat tacatatccg caaggtggat ggctgtgtcc
    35581 agcagaactg acctgtaatg tgctgaaact ggcgcaacag caaggtttac agattcgtta
    35641 tcaacaccag ttaaaggatt tatcccgtaa ggatgactgt tggttgctga attttgcagg
    35701 agatcagcaa gcaacgcata gcgtggtggt actggcgaac gggcatcaaa tcagccgttt
    35761 cagccaaaca tcgtctctgc cagtgtattc ggttgccggg caggttagcc atattccgac
    35821 aacgccggaa ctagcggagc tgaagcaggt gctgtgctat gacggttatc tcacgccaca
    35881 aaatccggcg aatcaacatc attgtattgg tgccagttat catcgcggca gcgaagatac
    35941 ggcgtacagt gaggacgatc agcagcagaa tcgccagcgg ttgattgatt gtttcccgca
    36001 ggcgcagtgg gcaaaagagg ttgatgtcag tgagaaagag gcgcgctgcg gtgtgcgttg
    36061 tgccacccgc gatcatctgc caatggtagg caacgtgccc gattatgacg taacgctcgt
    36121 ggaatatgct tcgctggcgg agcagaaaga tgaggcagta agcgcgccaa tttatgacga
    36181 tctttttatg ttagctgctt taggttctcg cggtttgtgc tctgccccgt tgtgtgccga
    36241 gattctggcg gcgcagatga gcaacgaacc aattccgatg gatgccagta cgctggcggc
    36301 gttaaacccg aatcggttat gggtgcggaa gttgttgaaa ggtaaagcgg ttaaggcggg
    36361 gtaatcagct ctggcattgc ttgccggata tggcgtgaac gccttatccg gcacattact
    36421 tactgctgtg acgccttctg aaacaggttt tcccacatat cggtcaccag cgcctggtca
    36481 cgcggcgaca actcaccggc accaatcgct ttttccagac tctggctaac ggttgtatgt
    36541 accgcctggg cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg
    36601 ccacgcaaat acccactggc aaacaactca tcatcactgg catggtcaac cataccgtcg
    36661 attaatgcca gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg
    36721 tggcgctggc ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc
    36781 gtgtgataat aattttgtag tgctttaatg aagcgtgccg gacgttcggg aatgccgttt
    36841 gccagataat ccattacctg cgcatgtacc cgccgttgaa acaccacgcg gtccggttcg
    36901 aaatcccctt ccagattgtc gcagctaaca ttaaacggat atcccgccgc cacgcagaac
    36961 aaccagtcca gcgcctgcgg cttcacttca acatcttcaa actggctttg cgtctgggca
    37021 tcgcgaccgt ccgggcaata ccagtagccg aaatcaacct gctcacggcg cgcttttccg
    37081 gcaatacacc agtgcgaaat ctcatgaatt gcgctggcat aaaagccatg agcaaagacg
    37141 attcggttat acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta
    37201 atcagacggg tattaaattc atcggcaaag cagctattaa aaatctcaat caactgctca
    37261 tagtggtgtg tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc
    37321 tatcgtaaag taattttgca ctcatcaccg ccgagacgat aacgatcatc gggcggatca
    37381 gcttttgtcc tttgctcaac accagtcttg aacccatgcg cgcgccgagg aactgcccca
    37441 ccagcatcac aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac
    37501 cgccaatgtt tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga
    37561 atccgcacag cgtaacgaag gccagcgcgt aaaacgatcc ggcagctggc ccaaagaatc
    37621 catcgtaaaa accgacgcag ccccccgcca ccagcgcaaa tggcagtcca tacatccggc
    37681 gctggcgatc ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca
    37741 ccagaatggg cagaatctgc cgtaagacat cagcctgaac atactgcacc agtaatgcgc
    37801 cgctcattga gccgacaaag gtcatggcga tattgagttt ctgatcgctt aaactaacca
    37861 ctttgcggcg aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat
    37921 tggttgccag cgcgttagcc ggagacatcc ccgccgccat caatgccgga atggtgagta
    37981 acccaccgcc accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa
    38041 ctcccagcag cagcggggaa accataaaca ggctattaaa cgtttccatt agatcacgtg
    38101 ctcatccagt agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt
    38161 tgttgttccc ggttttggtg gttcaaacca gctttgcagt tctgccccgc aaccatcgcc
    38221 cggtggcggt aaaggttgat cttcacactc caggctatcg gcaggacaac gtagtcgtac
    38281 atgcatatgg gcgcgatgct ggaaccaggg ccgcactttg cgtaaccagt cgcgatcggt
    38341 gcccgcatca aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc
    38401 tttgtcctgc gcggcgagtt taatcaagct gaaaatttcc ggcttccaca gcgtcgggac
    38461 aacgtgttta ccgtcgcggg aaactaaatc taacgcttgc gggcgcaaga gctgcgcgga
    38521 ggtccagcgt gtttttggca gttgcaggaa gatatcgaca tccagtccag tctggtggct
    38581 ggcgtgaccg ccgttgaaac gaccaccagc aggcatcccc atatcgccaa tcagcaccgt
    38641 acccataccc agattgctca cctggcgact cagacgctgg ataaacatca ccagatccgg
    38701 gtgtccgaaa tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg
    38761 cagcgtgtca gcaccgacga tacagccatt tgaaaaactg cctatcgatt gtgcactacc
    38821 cggcacaggt tgggttattt tttgccacgg cgtcgctgcc aggctggcgc tactggcaag
    38881 cagggccagc agcacaatcg cagttttgtt catttttacc agcgtggaat atcagtcttc
    38941 acatcagcat tttgcgcccg ttgccgtaac aggtgatcca ttaaaacgat cgccagcatc
    39001 gcttcagcga tcggcaccgc gcggatccca acacacggat cgtgacggcc tttggtgatc
    39061 atctcaactt cttcgccaaa gcggttaatc gtgcgccccg gcacagtaat gcttgaggtc
    39121 ggtttcagcg ccatatgggc aatgatttgc tgcccgctgc tgataccacc aagaatgccg
    39181 cccgcatggt tgctctggaa accgtctttg gtgatttcgt cacggttctg gctgccacgc
    39241 agcgctacca cgtcaaaacc atcaccaatt tccacacctt tcaccgcgtt aatgctcatt
    39301 agcgcatggg cgatgtcggc atccaggcgg tcaaaaaccg gctcgccaag tccggcgggt
    39361 acgccactgg caacgacggt gactttcgcg ccgatggagt caccctcttt tttcagcgcg
    39421 cgcatcagtt catctaacgc gtcgattttg tccgggtccg ggcagaaaaa cggattttgc
    39481 tcgacctgcg accagtcttt gatttccagc ggaatatcac ccatctgggt caggcagccg
    39541 cgaatctcaa tgccaaattt ctcggcgaga tattttttgg caatcgcccc tgctgcaacg
    39601 cgcatagcgg tttcgcgggc ggaagaacgt ccaccgccgc gataatcgcg caggccgtat
    39661 ttttgttcat aggtgtaatc ggcatgacct ggacggaaaa cgtccttaat cgcactataa
    39721 tcctgagaac gctggtcggt attttcgatc aacaatccga tgctggtgcc agtagtaaca
    39781 ccttcaaaaa caccggagag aattttgacc tgatccggct cgcggcgctg ggtggtatag
    39841 cgcgatgtcc caggtcgacg acgatcaagg tcatgttgca agtccgcttc cgtcagcgga
    39901 atgcctggcg gaacaccatc gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag
    39961 gtggttacgc gaaagagttg tccaattgtg tttccagcca tcacggctcc gttattgttg
    40021 tgtttgcgtg tttacttaat ctttataaat cgcgaaatgt tctcgtgcgg caataagctg
    40081 ctctttagtg agcataaaca caccatcgcc gccgttatca aactccagcc aggtgaacgg
    40141 aacatccgga tattgttcca taagatgtac catgctgttg cccacttcac aaatcagcac
    40201 gccatcatca gcgaggtaat ctgccgcgtt accaagaatg cgacgcgtca gtttcaggcc
    40261 gtcagtgcca gatgccagac cgagttccgg ctcgtggcgg tattcgtttg gcaggtcgga
    40321 catatcttcc gcatcgacat acggcgggtt ggtgacaatc aggtcgtact gcactttcgg
    40381 caaatcacgg aacaagtcgg aacgaatcgg aatgacgttg tggatcaaac cgtgttcttc
    40441 gatgttctgt tcagcaaccg ccagcgcgtc gggagagatg tctaccgcgt cgacttccgc
    40501 ttccgggaag gcataagcac aggcaatggc gatgcagccg ctaccggtac acatatctaa
    40561 aatgtgctgc ggttgcttgc tgatgagtcc ggcaaattta ttgttgatca gttcaccaat
    40621 cggcgagcgc ggcaccagca cgcgttcatc gacgtaaaat tcatggccac agaaccaggc
    40681 tttattggtc aggtaagcca ccggaatgcg ttcgttgacg cggcggatca cgcgctcaac
    40741 aatacggtgt ttttcgctgg aagtcagacg cgcggtgcgc atatcttccg gaatatccag
    40801 cggcaggtag agtgaaggca acaccagttg cacggcttca tcccacgggt tatcggtgcc
    40861 gtggccgtac cagatattcg ccgcgctgaa gcggctcacc gaccagcgca acatgtcctg
    40921 aatggtttgc agctcattta ctgcttcatc aacgaaaatt ttatccacgt attcctccag
    40981 ggcatgatcg caataatttc ggcggctagt ttgccatgaa gatgacgata aatcagcatt
    41041 cacgcgcggt gagtgaggaa aaatacgttt aaaacgatcg attgtgctac gagtcaggta
    41101 aactgtagga aaattagaaa tagagacagt taaatgaaaa agaaaacaac acttagcgag
    41161 gaggaccagg cgctgtttcg ccagttgatg gcggggactc gcaagattaa gcaggacacg
    41221 attgtccacc gaccgcagcg taaaaaaatc agcgaagtac cggtcaagcg gttgattcag
    41281 gagcaggccg atgccagcca ttatttttcc gatgagtttc agccattatt aaataccgaa
    41341 ggtccggtga aatatgttcg cccggatgtc agccattttg aagcgaagaa actgcggcgc
    41401 ggtgattatt caccggagtt gtttcttgat ttgcacggtc tgacgcagtt gcaagccaaa
    41461 caggaactgg gggcgttgat tgccgcctgc cgccgtgaac atgtgttttg tgcctgcgtg
    41521 atgcatggtc acgggaagca tattttgaag cagcaaacgc cgctgtggct ggcgcagcat
    41581 ccacatgtaa tggcatttca ccaggcacca aaagagtatg gtggcgatgc ggctttgttg
    41641 gtgttgattg aagtggaaga gtggctgccg cctgaattgc cctgaatgct ggcatcgaat
    41701 gctgtagagg gaaagttcgc cttagccatc atatcataaa acagacgccg gaaatgacga
    41761 cagggccaga tggccccgtc gacttgtgaa aatatcgtca ctacaattca ttcagtacat
    41821 actggaatcg gcacgtagaa tcaatcggtt gtcggctact ggtgttcaca tttaccacga
    41881 tatcatgtag ctgtttatcg gttaccggat gccgtgtggg agaaaccgtt accgttgatt
    41941 tatcgcaacc gtaggaggta aaggttaaca gttccgagac cacgcggcag tatgcacctc
    42001 tgctatcaaa aacgctgcca tcacagagtt tgccatcttc catcaggtag gtcgaaagcg
    42061 tggtggtgat tgtcgagcca ttggattgca actgtaacgc cttattgtgg ctgacgtaat
    42121 tctcactcca cgagatgtca tacgacgcga cagggatcgt cggacatact gtggcatggt
    42181 tcgttggccc agtatatctc ccgaatccgg gttgatctgt gatcgtataa aaagaagcga
    42241 gccacgggca accataactg tcggactgaa cggttatctt aaacgtctca tatgcacact
    42301 gaaatgtcgc cgtatttatc ccaggacaat tcgagctatc agcattaccg tagtcagtta
    42361 agatcaacac tggcgctggt cccgaccccg tccacgaaat aacgctggct tgctcgttct
    42421 tccttatggc atcgttgtac accccatagg ccgcctcgtt gggagttaac accgtgacat
    42481 acaaacctcc ggtgttatga atagacattt tccccttccc ataataaatc ttcacactgt
    42541 cgaaactcgc gctggtgagt ttatccccgg catagctctt tggaacgctc gcactcatca
    42601 ccagcaacac caggctcgca aaaatcattg tccatttttt catgttaaaa atactcaaag
    42661 gttgccgtcg ccgtagcgct gaaatcaccg gacgtcacat cccgcccgcc cttcgcctgt
    42721 agccaggcat taaaattgag actgttattg ccctggttta gcgggaacgt gacacccgtt
    42781 gtgctgttga ctggcaccgc cgcgcctgcc gccgtttcaa taccaatccc agctccggat
    42841 gcagaagatg aggcgtcaaa ggccagaaat ccgggcagtt cgctatcttc cgttcccgtc
    42901 agcgttaccc tgacgctaaa cagcgtggag cttttgcaat ccttcaattg aaataccaac
    42961 ggtactcgct ctgactgccc gaggttcatt aaatcgctgg cggcaatggt gggaaagtgc
    43021 acctccgcca acaggctacc gttgatcact ggcgtacagg atttaccgag caaattaccg
    43081 gtaaagtgca ggttgtcgac cgccggggca gtaacgctgt acaacagcat caggctacag
    43141 ataaaaacca acgcacgctt tttcttcatt gataatccac ctgtaacgtg gcccaggctt
    43201 caaagtctgc ctcaggcaaa tcgacaccgc tctttttgac cggtactgcg gttaacaccg
    43261 gtaaggccgt ttcattcacc accagtgccg tattgagctt aaagctctgc cctgcctgtt
    43321 taagccgaat cgccaggcca gagatatcgg tgctaaccgc ggcatcgtca aagtcgctct
    43381 gcgtaccgct ccagctcaat ttcatttcca ttgatgggtc gcgaaccgcc gtatcacagg
    43441 taatggaata cggcacggtt tgctcatagt tactgccatt aatgtcatca acaaaaacat
    43501 cctgaaaggt tatttcaatg gttttaccgt tgctaatagt gcagttaggt ggctgcacta
    43561 aatagccgtg aaaagagaca tttgagagtg ccatcgagct aaagccccac agcataagaa
    43621 gtgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa attccgctgc
    43681 cgataaggtt gtaccactct gtttcaccgg cactgcttcg agtgcaggga cgcccgagga
    43741 aaaattaaac ggcaaccatg aaccagacgt taagtcgagt gttgagtgat cactactttt
    43801 ctgaacccga ataccaaacc ctgaaatacc tgtgctgata actgtttcac cgttgatcac
    43861 aaccgttgtt gcccgcatct gtaaacgcag gtcatccatt gccaggttgt tacaaacaag
    43921 cgagtaatca atgggttgtt tataacctac cccatcgatc ttattgatga atacattacc
    43981 aaactcaacc gtcgcaccat taaccgtgca aggaggaggg gcatcaacga gcactcgtaa
    44041 actcaaattt actgatttac tccctgcaac tgcaaagtcg ctaccaatac aacacatcag
    44101 taaccatgtt cccgcccaga ctgaacaaga tcttttcata cttacttccc cgtcattaac
    44161 ctttgcggct cagctcttca tcaacctgac aactgttgcc gctacaggtg aaaaagagtg
    44221 gcatccgtgc gccatagtcg ttgacatagg tcagcaccgg cacgctagcc atcttcacct
    44281 tgagcggtgc ggaggttttt ggcgacatca ctatcggcga gaaaccgtct gccggattgc
    44341 catttttttg cgcgctggca ttactgaaaa tgacgtaata cggagtaggg ttattgacgg
    44401 taaattcctg accgttacgg gttaaggtca ctttatgctg ccatgggttt tgcatgctag
    44461 ctttttccag ggctttaggt cgccagaaaa gtttgatgcg tgtctgcagc gcaatttgta
    44521 acgtattggc tttgttcgat ttcggcggaa tttcacgcac attgaaataa aatatcgatt
    44581 cacgatcggc aggcaattta tggatatctg gcaatccctg aattttcacc tgaccattca
    44641 tcattgcatc aatgcgctgt acggggggca atactgccag cggcgaacta atcttgacgc
    44701 ccttttcatc ttcgatccag ctttgtgcca gataaggctg tttcgggtcg ttattacgca
    44761 gcgtgacgct gatggattta tcgctttcat taaaaatcag gcgagtacga tcgggcgtta
    44821 ctgacgccag agcagaaaaa cttaccgttg cagccagtaa cgacagtacc gttgccaatg
    44881 ttttgacaga gagatgttta gtcatggatc attccttaat cacgttattg gttcgatgaa
    44941 acagacatcg gcggttccat tggtgtcacc tgttgtgtct gctcctgaat tacaggttga
    45001 atatcatgcg ttatgacggg agcacttccc cctttttgct ggcatggcaa caacaagcca
    45061 ttgaacaggt cattcggtaa cggatctggc aggctgatat cgcagtgaga ttctccctcc
    45121 cagaaaacgg tcatatgttc acccggctta acgcctgcaa gataaacatt accttcgtca
    45181 tcaaccagcc caacttgttg ttgattatcg tttttcactt ccgccccaaa tggcggatgg
    45241 ctgccatcct gcaagcgcag cactaccatc gccttctcac cgctgatcac cgcaaattta
    45301 cggtagccaa tagcgccttc cgtcagcgtt gcctggacca ccgattgggt agcttcggcg
    45361 ttttccggta gtttgttcaa atcgatataa gcctggttac ggtagtaatc attaacatct
    45421 gccaccaccg ctttcccgaa catgttggta taaacggcgg aaccattacc ttcgaccggc
    45481 acattagcaa cgccatcggc gtcgattagc agacgtgtgc ctcccatatt ttgcgtacga
    45541 tgcaacgccc cgccgtgagc agtcagtgtt acaccgccct gtaaggagac ccccgcagat
    45601 gtgtattgcc cctcatggta gtttgcactc aggtcaactt ttgccagcgt actgtcatga
    45661 ctgtaataac catcaagact ggtgtgattt tcactggttc cggcgctcaa cgtatagtgg
    45721 ctagcgtcat cgatgcggtg atacaaactc gcctggctgc tgtcggcacc actgccataa
    45781 ttgccgttgt aactgatggt gctgttgtcg ccccatggca aactcaagga cagataaaca
    45841 cctttgtcgg cacttttatc atattcgtag cgataaccgg tgagagaaac gctcaggttg
    45901 cgaagacttc ctaaattgaa ataatgagaa agcatcatgt tgtagttggt ctgatcctcg
    45961 cgatcccaat aggtatggtg agaataattg agatagaccg aaacgcgcgc atccctaaag
    46021 ttctggttgt aagtgaccgt gtacatttcc ttgtcgttac ccgtgcgcgc acggtcggtg
    46081 tcgttcgcat ccagatactc actcatggtc atgtagtttt tttcagagaa gcgatatccg
    46141 gcaaacgtca cccgactgtt gagttcatcg aaatctttgg catagctgac gcgataagag
    46201 ttgccgtcca gcgttttgtt gccatacacg ctgttatcgt cgagttgaac acgagagtga
    46261 gtgacatcaa acgctagcgc tccgaacagc gcgaggtcac gaccgaggcc taacgctgcc
    46321 gactggtagt tttcatcggc caacgccccg ccgtagagcg accagccatt cgcaatcccc
    46381 catgacgcct caccgcccga gaagaagctg cctgcaacct gatggtccca gttttgtgga
    46441 cgccccattg tcactttgta acgcacctga ccggggcggg tcagaaacgg catggacgcc
    46501 gtgctgacgt catattcctg tacctgaccg ttttgttctt caatgcggac atgcagcgtg
    46561 ccagatacgg aatcgccaat atcctgaatg cggaatggac ctgcgggcac ttgggtttcg
    46621 taaatgacac ggcccatttg ggttacggtc actttagcgg tagtgcgtgc aacacctgaa
    46681 atatcgggtg cgtagccgcg catgtttggc ggtaacattt gatcatcggt actgatgctg
    46741 ccgccaatgt aactgaagcc atcaaaaata tcggagttga gataatcttc acccagcgaa
    46801 agtttggctt ttaatgacgg caaggcgcgc caggcgtagt aacggctcca ctcccagttt
    46861 ttttgcgtac cgctatcatc agtgtcgccg tcatcgttac tgcgggtatg ttggtaatcg
    46921 ctctgccagt cggctcgtaa gcgccacggc cccacgttaa cgccgaccgt accattaccg
    46981 ctgatgtcgt tagtgtcatc ccccccattt tcatgtcggg tttgtgcgtt gatactgtaa
    47041 tcggcaataa gcccaggaat cccctcatcc caacgcgaag gtgggtccca gtcatcgtca
    47101 gtgtattcga gatatgcctg tggcacagaa actaagagcg cagattgact taaatcgccc
    47161 ttaatttcca tacctcctat ttggtcagtt ttcagacatt gtccatcatg accccattgc
    47221 aaactcttcg caaaatcatc tttgaagcca aactgggcta tcagttcagg tggtaagcag
    47281 gcatatgatt tattcggatc gttttcagct acataccaat agatatcgta ttcatcaggt
    47341 aatgggtgat tatttacatg aacacgtaaa ttgtatttcc ctggttcaac ataccctttt
    47401 tgagaaaaac gccccagatc aattttggta tttcctttta actccagaaa tcgagcatca
    47461 aattgaatgg tatcatctgc cagtacatta aaaggactcc cggaaattgc cagagcaatc
    47521 caccatgaaa gcccccgtaa acgaagaaaa gaatgttcag gcatctacgt atccatccat
    47581 gattattata atttagttat cttttattta taaaccccat aacattgcag ttatggggga
    47641 acagcataat tagagatatg taatctggaa cgtcgttagc gtttcaaacg gcgctggggt
    47701 tggtgttacc gctgcagttt cacctactaa ccaggcttta aagttcaacg tttgtttctg
    47761 tttgcctttt cctgttgaac tgtccatcgc gatgtcctga tcgatgcctg cccccaggtc
    47821 ataaccagta ccacgttggt cgccaatcac cagacttaca tttggaattt cagttccatt
    47881 atccatgtta gacagggaat agttttttga gttgccggag tacggattac cagtgaatgt
    47941 cgtggtcatt gtcgtttcag tattgaaatt acaatcctgt aaacgaatct ggaaatcttt
    48001 aggtgtggta gtcttgccac cggttaaaga acctgtacca atgctgccca tatccacttc
    48061 cagtttatga tcgtcaggaa caatcgagca cggagagtct tcaattgtgc cgtaaaatct
    48121 cactgtaccg ttgttacctg cggcaacgac tgatgctgaa gtcagcgccc ccatgagcat
    48181 tgccgtggca actgctgctt tta
//
LOCUS       DAZUCC010000010        46404 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZUCC010000010 DAZUCC010000000
VERSION     DAZUCC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.010"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..492)
                     /gene="yjgL"
                     /locus_tag="ACHY09_000491"
     CDS             complement(<1..492)
                     /gene="yjgL"
                     /locus_tag="ACHY09_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFS3883508.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNDFARIAGGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVEIEITLSTITTMGTRQGHTAIIFQQSDGSTNRYEGKSFERKDD
                     SSLH"
     gene            complement(615..1067)
                     /gene="tabA"
                     /locus_tag="ACHY09_000492"
     CDS             complement(615..1067)
                     /gene="tabA"
                     /locus_tag="ACHY09_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFS3883509.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKANVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(1212..1805)
                     /gene="bdcR"
                     /locus_tag="ACHY09_000493"
     CDS             complement(1212..1805)
                     /gene="bdcR"
                     /locus_tag="ACHY09_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS3883510.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILEE"
     gene            1876..2589
                     /gene="bdcA"
                     /locus_tag="ACHY09_000494"
     CDS             1876..2589
                     /gene="bdcA"
                     /locus_tag="ACHY09_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3883511.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2721..3116
                     /gene="yjgH"
                     /locus_tag="ACHY09_000495"
     CDS             2721..3116
                     /gene="yjgH"
                     /locus_tag="ACHY09_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFS3883512.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            3397..3531
                     /gene="pyrL"
                     /locus_tag="ACHY09_000496"
     CDS             3397..3531
                     /gene="pyrL"
                     /locus_tag="ACHY09_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFS3883513.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3535..4470
                     /gene="pyrB"
                     /locus_tag="ACHY09_000497"
     CDS             3535..4470
                     /gene="pyrB"
                     /locus_tag="ACHY09_000497"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFS3883514.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4483..4944
                     /gene="pyrI"
                     /locus_tag="ACHY09_000498"
     CDS             4483..4944
                     /gene="pyrI"
                     /locus_tag="ACHY09_000498"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFS3883515.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5017..5403
                     /gene="ridA"
                     /locus_tag="ACHY09_000499"
     CDS             5017..5403
                     /gene="ridA"
                     /locus_tag="ACHY09_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFS3883516.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5609..8305)
                     /gene="mgtA"
                     /locus_tag="ACHY09_000500"
     CDS             complement(5609..8305)
                     /gene="mgtA"
                     /locus_tag="ACHY09_000500"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS3883517.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGVYSRRYGWQ"
     gene            complement(8446..8499)
                     /gene="mgtL"
                     /locus_tag="ACHY09_000501"
     CDS             complement(8446..8499)
                     /gene="mgtL"
                     /locus_tag="ACHY09_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFS3883518.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8684..9631
                     /gene="treR"
                     /locus_tag="ACHY09_000502"
     CDS             8684..9631
                     /gene="treR"
                     /locus_tag="ACHY09_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFS3883519.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            9750..11171
                     /gene="treB"
                     /locus_tag="ACHY09_000503"
     CDS             9750..11171
                     /gene="treB"
                     /locus_tag="ACHY09_000503"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFS3883520.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11221..12876
                     /gene="treC"
                     /locus_tag="ACHY09_000504"
     CDS             11221..12876
                     /gene="treC"
                     /locus_tag="ACHY09_000504"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS3883521.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKASPYRQFYIWRDGESETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRRLMTVGEMSSTSLEHCQRYATLTGSELSMTFN
                     FHHLKVDYPSGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13261..15399
                     /gene="nrdD"
                     /locus_tag="ACHY09_000505"
     CDS             13261..15399
                     /gene="nrdD"
                     /locus_tag="ACHY09_000505"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS3883522.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRSQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15557..16021
                     /gene="nrdG"
                     /locus_tag="ACHY09_000506"
     CDS             15557..16021
                     /gene="nrdG"
                     /locus_tag="ACHY09_000506"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFS3883523.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16066..16452)
                     /gene="cybC"
                     /locus_tag="ACHY09_000507"
     CDS             complement(16066..16452)
                     /gene="cybC"
                     /locus_tag="ACHY09_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFS3883524.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16635..17987)
                     /gene="pmbA"
                     /locus_tag="ACHY09_000508"
     CDS             complement(16635..17987)
                     /gene="pmbA"
                     /locus_tag="ACHY09_000508"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFS3883525.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18081..18632
                     /gene="yjgA"
                     /locus_tag="ACHY09_000509"
     CDS             18081..18632
                     /gene="yjgA"
                     /locus_tag="ACHY09_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFS3883526.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIEQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18790..20163)
                     /gene="mpl"
                     /locus_tag="ACHY09_000510"
     CDS             complement(18790..20163)
                     /gene="mpl"
                     /locus_tag="ACHY09_000510"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFS3883527.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20339..21337
                     /gene="fbp"
                     /locus_tag="ACHY09_000511"
     CDS             20339..21337
                     /gene="fbp"
                     /locus_tag="ACHY09_000511"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFS3883528.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21370..22365)
                     /gene="yjfF"
                     /locus_tag="ACHY09_000512"
     CDS             complement(21370..22365)
                     /gene="yjfF"
                     /locus_tag="ACHY09_000512"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFS3883529.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLLIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22352..23374)
                     /gene="ytfT"
                     /locus_tag="ACHY09_000513"
     CDS             complement(22352..23374)
                     /gene="ytfT"
                     /locus_tag="ACHY09_000513"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFS3883530.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(23388..24890)
                     /gene="ytfR"
                     /locus_tag="ACHY09_000514"
     CDS             complement(23388..24890)
                     /gene="ytfR"
                     /locus_tag="ACHY09_000514"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFS3883531.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25030..25986)
                     /gene="ytfQ"
                     /locus_tag="ACHY09_000515"
     CDS             complement(25030..25986)
                     /gene="ytfQ"
                     /locus_tag="ACHY09_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFS3883532.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26296..26826
                     /gene="ppa"
                     /locus_tag="ACHY09_000516"
     CDS             26296..26826
                     /gene="ppa"
                     /locus_tag="ACHY09_000516"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFS3883533.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27206..27547)
                     /gene="ytfP"
                     /locus_tag="ACHY09_000517"
     CDS             complement(27206..27547)
                     /gene="ytfP"
                     /locus_tag="ACHY09_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFS3883534.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(27550..31329)
                     /gene="tamB"
                     /locus_tag="ACHY09_000518"
     CDS             complement(27550..31329)
                     /gene="tamB"
                     /locus_tag="ACHY09_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFS3883535.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSMPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(31326..33059)
                     /gene="tamA"
                     /locus_tag="ACHY09_000519"
     CDS             complement(31326..33059)
                     /gene="tamA"
                     /locus_tag="ACHY09_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFS3883536.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33265..33903
                     /gene="msrA"
                     /locus_tag="ACHY09_000520"
     CDS             33265..33903
                     /gene="msrA"
                     /locus_tag="ACHY09_000520"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS3883537.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34226..35569
                     /gene="paeA"
                     /locus_tag="ACHY09_000521"
     CDS             34226..35569
                     /gene="paeA"
                     /locus_tag="ACHY09_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS3883538.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(35631..35837)
                     /gene="ytfK"
                     /locus_tag="ACHY09_000522"
     CDS             complement(35631..35837)
                     /gene="ytfK"
                     /locus_tag="ACHY09_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFS3883539.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36162..36719
                     /gene="ytfJ"
                     /locus_tag="ACHY09_000523"
     CDS             36162..36719
                     /gene="ytfJ"
                     /locus_tag="ACHY09_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFS3883540.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(36709..37449)
                     /gene="cysQ"
                     /locus_tag="ACHY09_000524"
     CDS             complement(36709..37449)
                     /gene="cysQ"
                     /locus_tag="ACHY09_000524"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFS3883541.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAVAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37639..39582
                     /gene="cpdB"
                     /locus_tag="ACHY09_000525"
     CDS             37639..39582
                     /gene="cpdB"
                     /locus_tag="ACHY09_000525"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFS3883542.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAVFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39711..40091)
                     /gene="ytfH"
                     /locus_tag="ACHY09_000526"
     CDS             complement(39711..40091)
                     /gene="ytfH"
                     /locus_tag="ACHY09_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS3883543.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40180..41040
                     /gene="qorB"
                     /locus_tag="ACHY09_000527"
     CDS             40180..41040
                     /gene="qorB"
                     /locus_tag="ACHY09_000527"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFS3883544.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41148..42113
                     /gene="ytfF"
                     /locus_tag="ACHY09_000528"
     CDS             41148..42113
                     /gene="ytfF"
                     /locus_tag="ACHY09_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS3883545.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPGFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            42221..42883
                     /gene="ytfE"
                     /locus_tag="ACHY09_000529"
     CDS             42221..42883
                     /gene="ytfE"
                     /locus_tag="ACHY09_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFS3883546.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(42928..44340)
                     /gene="cycA"
                     /locus_tag="ACHY09_000530"
                     /gene_synonym="dagA"
     CDS             complement(42928..44340)
                     /gene="cycA"
                     /locus_tag="ACHY09_000530"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFS3883547.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44649..45269)
                     /gene="fklB"
                     /locus_tag="ACHY09_000531"
     CDS             complement(44649..45269)
                     /gene="fklB"
                     /locus_tag="ACHY09_000531"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS3883548.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45487..46125
                     /gene="ytfB"
                     /locus_tag="ACHY09_000532"
     CDS             45487..46125
                     /gene="ytfB"
                     /locus_tag="ACHY09_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFS3883549.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46109..>46404)
                     /locus_tag="ACHY09_000533"
     CDS             complement(46109..>46404)
                     /locus_tag="ACHY09_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS3883550.1"
                     /translation="YAKSQRLLEINHAHLQLMESLLDEGKKNNIFKPDIDPLQVNINI
                     AALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
BASE COUNT        11322 a        12419 c        11629 g        11034 t
ORIGIN      
        1 gtgtaatgaa ctatcatctt ttctttcaaa agacttccct tcataacgat tagtcgaacc
       61 atcagattgt tgaaatataa tagccgtatg tccttgacga gtccccatag tagtgatagt
      121 ggaaagtgta atttctattt ccaccttaga taaatccgct cgttcccctt caggattatt
      181 catgtaaaac ttcatttttt cattgccatc agtatcttta gaataagatg aggactcaat
      241 atgagccatc atatttctac ccgcttcgtt ggagcgtttg ctaaaatcca cataacccag
      301 ggtaaatatc tttgccaaaa actctaaaaa tgctccagga gaaagaatat tccttctgct
      361 atcctgcccc ccggcaatac gggcaaaatc attcattcca acacgccagg tatttaaaac
      421 ttcacttttt tgtttaaaat tgtcggctaa tgtaatgtgt tgagaataat tcaaatctga
      481 tattttgctc atgacgaact ccttgagtta ataagaacgt ctaagcatta caaattttac
      541 tcacaaaaaa atatcaaaaa acgctctttt cacaaactcg ccgtaaacct ggagttattt
      601 acggcgaaaa gtcatcacgc catcagcatc ttcactactg ctttgcgaac ccgggctggc
      661 gcgcccactg cgcacagcgg tttatgcact tcccccggat aaaacacaac aaaatcacct
      721 tcattaagga taactgtttt ctcatcaacg ccttccggca aaaatgcgat gtctttatca
      781 gctaaccagt cggtatccgg cgtacctgca ggttgtgtgc tgaaggtcat gccttcctga
      841 ccttttaaca caatctgaat gtcgagatag cgggcatggt actccgcacg gcgagcttcg
      901 tacggctcgg tcatatcttc cgagataaga taaaacagac gattgccttc gatatcgtgc
      961 ttgcccttgg gcgtttctgc cgtaacgttt gctttgatat gctcaatcgc ctggcgtaac
     1021 tcttggggta gccacggctg aagattatga atatttccga tgatcatctt ttccactcct
     1081 taattaaaac aacgtttcat ttctatcgtt atacgaaaag atgatcaatt ccgctagcct
     1141 taacgcgtga attattgtct tatcacaact tatcggcagg cgcgttcccg cctttcgggt
     1201 caaggcccgc atcactcctc gagaatagtt tttatcgctt cccccgccag ggcagctgtt
     1261 gcacagagtt gttcgatgga gtgcccctcc cgtgccttcg cagaaagccc tgacatcacg
     1321 gtactcataa aatcagtcac acattgtgcg ctttgtggat gccgcctggc gatatagtca
     1381 taaatggtcg tttctgcggc gtgataatat tgaatggcaa tatcacgcgc ttgtggatca
     1441 tgactatgaa taccttcaag aaccatacag ccagcgcagc cgccgttttg gctatatctg
     1501 cgcgccgctt cttttaatac ctcaaccagg cactcgccta ccggacgatc atcacgaaga
     1561 atatcggcaa gcggaatagc ttccgtaccg acatattcat tgagtacacg gctaaacaac
     1621 ccagccttac tgccaaaagc cgcgtagagg ctcggggggt taataccaag ataatcagta
     1681 acttcagaga cacttactgc atcgaaacct ttttggtgaa aaagcacttt tgccgcagag
     1741 actgcctgct caggagcgaa acgtctgggg cgacctggaa cgcgagattg ttttttagtg
     1801 accattacaa aacttgttga cagaaagtta aaacagtttt gtaatgcatg ttacataata
     1861 aatcaaggag tccttatggg cgcttttacc ggtaagacag ttctcatcct cggtggtagc
     1921 cgtggaatcg gtgccgctat cgtacgtcgt ttcgtcaccg atggagccaa tgtacgattc
     1981 acctatgcgg ggtcgaaaga tgccgctgaa cgtctggcac aagagactgg agcgacagca
     2041 gtattcacag atagtgctga cagagacgct gttattgatg tcgttcgtaa gagcggcgca
     2101 ttggatatcc tggtggtaaa tgcaggtatt ggcgtctttg gcgatgccct ggaattaaat
     2161 gccgacgata ttgatcgcct tttcaaaatc aatattcatg ctccttatca tgcctctgtt
     2221 gaagccgccc ggcagatgcc cgaaggcggg cgcatcttaa tcatcggctc cgtgaatggc
     2281 gatcgtatgc ctgttgcagg catggctgct tatgccgcca gtaaatctgc cctgcaaggc
     2341 atggcgcgcg ggctggcccg tgattttgga ccgcgaggga tcaccatcaa cgtcgtccag
     2401 ccaggaccaa ttgataccga cgctaatccc gccaacgggc caatgcgcga tatgttgcat
     2461 agttttatgg ccatcaaaag acatgggcaa ccggaagagg tcgctggtat ggtcgcatgg
     2521 ttagcagggc cagaagccag ttttgttacc ggcgcgatgc ataccattga tggcgcgttt
     2581 ggcgcataac cgactacgct caattaagcc cagccattat ccatgatgtc tgggttttgt
     2641 ttactaacgt cgtcccccta aaagcggctc ccggtaaata taaatcttct gttcatgtcg
     2701 taaatcttga ggataaccaa atggtagaaa gaaccgctgt tttccctgct ggccgacatt
     2761 cactgtatgc tgagcatcgt tattctgcgg ctattcgttc cggcgatttg ctgtttgttt
     2821 ccgggcaagt aggaagtcga gaggacggaa caccagaacc cgattttcag caacaagtca
     2881 gactggcatt tgataatttg catgcgaccc tggctgctgc gggatgcact tttgacgata
     2941 tcattgatgt tacgagcttt cataccgatc cagaaaaaca atttgaagac atcatgacgg
     3001 tcaaaaatga aatatttagc gccccacctt atccaaactg gacggcggtg ggtgttacct
     3061 ggctggcagg ctttgatttt gaaattaaag tgatagcgcg catccctgag ctataagcaa
     3121 tagtgttagc cgttcgtttt cacactccgc cctataagtc ggatgaatgg aataaaatgc
     3181 atatctgatt gcgtgaaagt gaaaaagaaa aaagcaggaa atgtctgcaa ttattgatac
     3241 cgaacgacag ttctccccca gaatcacatc aaataaaaat gcatgtattt tgacttttaa
     3301 ttcaaataaa ccgtttgcgc tgacaaaata ttgcatcaaa tgcttgcacc gcttctgacg
     3361 atgagtataa tgccggacaa tttgccggga ggatgtatgg ttcagtgtgt tcgacatttt
     3421 gtcttaccgc gtctgaaaaa agacgctggc ctgccgtttt tcttcccgtt gatcacccat
     3481 tcccagcccc tcaatcgagg ggcttttttt tgcccaggcg tcaggagata aaagatggct
     3541 aatccgctat atcagaaaca tatcatttcc ataaacgacc ttagtcgcga tgaccttaat
     3601 ctggtgctgg cgacagcggc gaaactgaaa gcaaacccgc aaccagagct gttgaagcac
     3661 aaagtcattg ccagctgttt cttcgaagcc tctacccgta cccgcctctc tttcgaaact
     3721 tccatgcacc gcctgggtgc cagcgtggtg ggcttctccg acagcgccaa tacatcactg
     3781 ggtaaaaaag gcgaaacgct ggccgatacc atttcggtta tcagcactta cgtcgatgcg
     3841 atagtgatgc gtcatccgca ggaaggtgcg gcgcgcctgg ccaccgaatt ttctggcaat
     3901 gtaccggtac tgaatgccgg tgatggctcc aaccaacatc cgacgcaaac cttgctggac
     3961 ttattcacca ttcaggaaac ccaggggcgt ctggacaatc tccacgtcgc aatggttggt
     4021 gacctgaaat atggccgcac cgttcactcc ctgacccagg cgctagcgaa gttcgacggc
     4081 aaccgttttt actttatcgc gccggatgcg ctggcaatgc cgcaatacat ccttgatatg
     4141 ctcgatgaaa aagggatcgc ctggagtctg cacagctcta ttgaagaagt gatggcggaa
     4201 gtagacatcc tgtacatgac ccgcgtgcaa aaagagcgtc tggacccgtc cgagtacgcc
     4261 aacgtgaaag cgcagtttgt tcttcgcgcc agcgatctcc acaacgccaa agccaatatg
     4321 aaagtgctgc atccgctgcc gcgtgttgat gagattgcga cggatgtgga taaaacgcct
     4381 cacgcctggt acttccagca agcaggcaac gggattttcg ctcgccaggc gttactggca
     4441 ctggttctga atcgcgatct ggtactgtaa ggggaaatag agatgacaca cgataataaa
     4501 ttgcaggttg aagctattaa acgcggcacg gtaattgacc atatccccgc ccagatcggt
     4561 tttaagctgt tgagtctgtt caagctgacc gaaacggatc agcggatcac cattggtctg
     4621 aacctgcctt ctggcgagat gggccgcaaa gatctgatca aaatcgaaaa tacctttttg
     4681 agtgaagatc aggtagatca actggcattg tatgcaccgc aagccacggt taaccgtatc
     4741 gacaactatg aagtggttgg caaatcgcgc cccagtctgc cggagcgcat cgacaatgtg
     4801 ctggtctgcc cgaacagcaa ctgtatcagc catgccgaac cagtttcttc cagctttgcc
     4861 gtgcgaaaac gcgccaatga catcgcgctc aaatgcaaat actgtgaaaa agagttttcc
     4921 cataatgtgg tgctggccaa ttaattgcgg ttggtaataa aagtctggct ccctataatg
     4981 agccagactt tttaccgctg taataaagga gaaatcatga gcaaaactat cgcgacggaa
     5041 aatgcaccgg cagctatcgg tccttacgta cagggcgttg atctgggcaa tatgatcatc
     5101 acctccggtc agatcccggt aaatccgaaa acgggcgaag taccggcaga cgtcgctgca
     5161 caggcacgtc agtcgctgga taacgtaaaa gcgatcgtcg aagccgctgg tctgaaagtg
     5221 ggcgacatcg ttaaaaccac cgtgttcgtg aaagatctga acgacttcgc aaccgtaaac
     5281 gccacttacg aagcgttctt caccgagcac aatgccacct tcccggcacg ttcttgcgtt
     5341 gaagttgccc gtctgccgaa agacgtgaag attgagatcg aagcaatcgc tgttcgtcgc
     5401 taatcttgat ggaaatccgg gctatcatgc ccggattaag tctgatgaca aaagcaaaat
     5461 cgcccgatgc gctacgctta tcaggcctac gtgattcctg caatttattg aatttgtagg
     5521 ccggattggg cgtttacgcc gcatccggca tgaacaaagc tcactttgtc tgcaatctga
     5581 atccgggcca ccatgcccag tttattcttt actgccagcc gtaacgacgg ctatagaccc
     5641 ctttcaccaa ctgggttaac gtcatatacc ctgccagaat cgcaaccagc cacgggaaat
     5701 agcttaacgg cagcgcctgc agctgcagat aactggccag cggtgaaaac ggcaatgcga
     5761 tcccgacaat catcacgatc acggtcatga tcattaacgg ccacgatgca cagctctgaa
     5821 taaacggtac acgacgggta cggatcatat gcacaatcag cgtttgcgac agtaagccca
     5881 ccacaaacca gcccgactgg aacagcgttt gcgtttccgg cgtgttggca tggaataccc
     5941 accacatcag gcaaaacgtc agaatatcga agatcgagct gatcggtccg aagaagatca
     6001 taaagcgccc cagatccgcc ggattccaac gctgcggctt ttgaatttgc tcgtcgtcga
     6061 cgttatcaaa cgggatcgcc acctgtgaca catcgtacag caagttctga ataagcaagt
     6121 gtaacggcaa catcggcagg aagggcaaga aagcactcgc caccagcacg ctgaacacat
     6181 taccgaagtt agaactcgcc gtcattttga tgtatttcag catgttggcg aaagtgcgac
     6241 gtccctcaat aaccccctct tccagcacca tcaggctttt ttccagcagg atgatatcag
     6301 ccgcttcacg ggcaatatct accgcgccgt ccacagaaat gccgatatcc gcagcgcgta
     6361 aggcgggcgc atcattaata ccatcgccca taaagccaac cacatgccct tcgcgcttca
     6421 gtaaggtcac aatgcgttct ttatgcatcg gcgtcaggcg ggcaaacagc gtggtgcgct
     6481 gtgcgagatt tgccagttca tcgtcagata gcgtttcaat atcactacca atgaccacct
     6541 ctcccgcatc cagccccact tcatggcaca ctttcgctgc aactaactca ctgtcgccag
     6601 taaggatttt tacggtaatt ccactcgctt ttaatgcctt cagtgccgga gcggttgtct
     6661 cttttggcgg atcaaggaaa gcaatatatc cttcgaggat caggtcggat tcatccgccc
     6721 gctggtaatc tccttcacgc gctggcaggt atttcgtcgc caccgcaacc acgcgcagcc
     6781 cctgacgatt cagcgtatca gtaacccgct taatcttacg cagcatgatg tcatcgagcg
     6841 gcacaatctc gccattgtga cgcacctgcg aacacacatt gaggatttcc tgcaatgcac
     6901 ctttgcaaac cagctgatgg tgttcggtat tttctgccac taccaccgac atccgacgac
     6961 gctcgaaatc aaacggaatc tcatcaattt tttgccaacg gctggccagc gaacgcgctg
     7021 actcttcatc cgtaccttcg agcaccgcgg tatcaagcag gtttttaagt ccggtctggt
     7081 aatgactgtt caaccacgcg ctatgcagca cgcgttcgct ggttttaccg gagatatcgg
     7141 tatgattctc cagcacaatt ttgtcctgcg tcagggtgcc ggttttatca gtgcacagaa
     7201 tatccattgc gccaaagttc tgaatggcat ccagatgttt gacgatcact ttctgtttcg
     7261 acagctttac tgcgccgcgc gccagcgtcg aggtaacaat catcggcaac atttctggcg
     7321 ttaagcctac cgcgaccgaa agcgcaaaca gtgccgcttc ccaccagtcg cctttggtgt
     7381 aaccgttgat aagcaacacc accggagcca tcaccagcat aaagcgaatc agcagcatac
     7441 tgacgcggct aatcccttgc tgaaaggcat tcggctcgct ttcctgctca ctaacacgcc
     7501 ccgccagttg accaaaccag gtattggccc ctgtggcaat caccattgct tgtgccgtac
     7561 cgctcaccac ggtggtgccc ataaaacaca gggtgtcgca ctccagcgga ttgctgtgct
     7621 ccggctggcg agtagtagcg gctttttcca cgggcagaga ctcaccggtt aacgacgcct
     7681 gagcgacgaa cagatcccgc gcctgcaaga tacgtaaatc tgccgggatc atatcccctg
     7741 ccgccagttt tataatatcg ccgggcacca gttggtcgat cgggatctcc agccagccat
     7801 tttcgccttt gtcgttaatt acgcgcagca ccgtcgcagt attgctgacc atcgctttca
     7861 gggcatctgc cgctttagtg gaacgtgctt cctgaataaa gttcagcaac gtagaaatag
     7921 cgaccattag cgcgataacg cccgcggcaa ataaatcttc cgtggcgtaa gaaatagcgc
     7981 cgagaatggt gagtaaaata ttaaaggggt tgcgatagca gacccataaa tgaacccacc
     8041 acggcgacgg ttgttgtgcg ggtaatttat tttcaccatg ttgttcgcgg gcagattcca
     8101 cttccgcctg atttaatcct tccggatggg tgtcgaacgt tttccacaat tcttcttcgg
     8161 gcatcaccgc cattttcagg caatgcgcac ttaaggacgg cgggaccgcc gtattcactg
     8221 tctgctgcgc gccaggcaat ggatcgcgat gaaccagacg ggaaggtaaa tggcgaatga
     8281 accgggtaaa aatttcttta aacataagga gtccctccgc actgtctgaa acagtacaga
     8341 aatgtcttca ggcgtggcga agccttacct gaaggtaata aagctgagca gggacgttaa
     8401 aacgtcacgg ccttacgcat ccggtaacgc cgtgaaaaga cgggcttacc ggaaaagttt
     8461 taatctccgt cgagggagag gcgtgggatc aggttccata taacctccgg taagtgaaaa
     8521 aagcgccgca aataataagg agaatccaat aatattcgta gcatttatgg cgatataata
     8581 cctgctggca actaaaccaa cgataaacca gactttaccc attgctgaat gcacaggtaa
     8641 agttaggctc aaataattaa acaacacgtt atgggacaac aggatgcaaa atcggctgac
     8701 catcaaagac atcgcgcgct taagcggcgt ggggaaatct acggtttccc gggtgctgaa
     8761 taacgaaagc ggcgtgagcc agcgcacgcg cgagcgtgtt gaagcagtga tgaatcagca
     8821 tggattttcc ccttcccgct ctgcgcgcgc tatgcgtggg caaagcgata aagtggtcgc
     8881 catcattgtt acccgtctgg attcgttgtc agaaaatctc gccgttcaaa ccatgctgcc
     8941 agcgttctat gaacaaggtt acgatccaat catgatggaa agtcagtttt ccccacaatt
     9001 agttgccgaa catttggggg tgctgaaacg gcgtaatatc gacggcgtag tgctgttcgg
     9061 ttttaccggc ataacagaag aaatgttagc ccactggcag tcatcgctgg ttctgctggc
     9121 gcgtgacgca aaaggctttg cttcggtctg ttatgacgac gaaggggcaa tcaagatcct
     9181 gatgcaacgg ctgtatgacc agggacatcg taatatcagt tatctcggcg tgccgcacag
     9241 tgacgtgacg accggtaagc gacgtcacga agcctacctg gcgttctgca aagcgcataa
     9301 attgcatccc gttgccgctc tgccagggct tgctatgaag caaggctatg agaacgtagc
     9361 aaaagtgatt acgcctgaaa ctaccgcgtt actgtgcgca accgacacgc tggcacttgg
     9421 cgcaagtaaa tacctgcaag agcaacgcat cgacaccttg caactggcga gcgtcggtaa
     9481 tacgccgtta atgaaattcc tccatccgga gatcgtcacc gtagatcccg gttacgccga
     9541 agctggacgc caggcggcct gccagttgat cgcgcaggta accgggcgca gcgaaccgca
     9601 acaaatcatc attcccgcca ccctgtcctg atcgtttcct gaacgataaa ttgtgatctt
     9661 cgctgcgttt cgggaacgtt cccgttttta aatttttcag cgcaatatat tctgcagcca
     9721 accaaaaatg tcatctgcca tggggcttta tgatgagcaa aataaaccaa acggatatcg
     9781 atcggttgat tgaactggtc ggcgggcgcg gcaatattgc gacggtgagc cactgtatta
     9841 ctcgtctgcg ctttgtcctc aaccaaccgg ccaatgccag accgaaagaa attgagcaac
     9901 tccccatggt gaaaggctgt ttcaccaatg ccgggcaatt tcaggtggtg attggcacca
     9961 acgtgggtga ttactatcaa gcactgatag cgtcaaccgg acaggcgcag gttgataaag
    10021 agcaggtaaa aaaagccgcc cggcagaata tgaaatggca tgagcagttg atctctcatt
    10081 tcgcggagat tttcttcccg ttgctgcccg cgttgattag cggcggtttg atcctcggtt
    10141 ttcgcaatgt gatcggcgat ttgcccatga gcaacggtca aacgctggcg caaatgtacc
    10201 cttccctgca aacgatctac gattttctgt ggttgatcgg tgaagcgatc ttcttctacc
    10261 tgccggtcgg gatttgctgg tcagcggtga aaaaaatggg cggcacgccg atccttggta
    10321 tcgtgcttgg cgtaacgctg gtttctccac agctgatgaa cgcttatctg ctcgggcagc
    10381 agctgccgga agtgtgggac tttggcatgt tcagcatcgc caaagtgggc tatcaggcgc
    10441 aggtgatccc ggcactgtta gccgggctgg cgctgggcgt tattgaaact cgtcttaagc
    10501 gcatcgtgcc ggattacctc tatctggtgg tggtgcccgt ttgttcgctg atcctcgcgg
    10561 tgttcctcgc ccatgcgctg attggtccgt ttggtcgcat gattggcgat ggcgttgcct
    10621 ttgcggtacg tcacctgatg accggcagct ttgctccgat tggcgcggca ttgtttggct
    10681 tcctgtacgc cccgctggtg atcaccggcg tacatcagac cacgcttgct attgatttgc
    10741 agatgattca aagcatgggt ggtacgccag tgtggccgct gattgcgctg tcgaatatcg
    10801 ctcagggttc cgccgtgata ggcattatca tttccagccg caagcacaat gaacgcgaga
    10861 tctccgtgcc tgccgctatc tccgcctggc ttggagtcac tgagcctgct atgtacggca
    10921 tcaacctgaa atatcgcttc ccgatgctgt gcgcgatgat tggttctggt ctggcaggat
    10981 tgctatgcgg cctgaacggc gttatggcga atggcatcgg cgtaggcggc ctgccgggaa
    11041 ttctctcgat tcaaccgagc tactggcagg tgtttgcgct ggcaatggct atcgccatca
    11101 tcatcccgat tgtactcacc tcgtttatct atcagcggaa ataccgcctg ggcacgctgg
    11161 acattgttta attttcttcg gggcgcaatt gcgccccctc gcatttgcag gaataacgta
    11221 atgactaatc ttccccactg gtggcaaaac ggcgttatct accagattta tccaaagagt
    11281 tttcaggaca ccacgggtag cggcaccggc gatttacgtg gcgttatcca gcgcctggac
    11341 tatctgcata aactgggcgt tgatgccatc tggctaaccc ccttttatgt ctctccccag
    11401 gttgataacg gttacgacgt agcgaactat acggctattg atcccaccta cggcacgctg
    11461 gacgattttg acgaactggt gacacaggca aaatcgcgcg ggattcgtat cattctcgat
    11521 atggtgttta accatacctc tacccaacat gcctggtttc gcgaggcgct gaacaaagca
    11581 agcccttacc gccagtttta tatctggcgc gatggagaat cagaaacgcc accgaacaac
    11641 tggcgttcaa aatttggcgg tagtgcgtgg cgctggcatg cggaaagcga acagtactat
    11701 ttgcatctct ttgcaccaga acaggcggat ctcaactggg agaatccagc ggtacgcgca
    11761 gagctgaaaa aagtctgtga gttctgggcc gatcgtgggg tcgacgggtt gcgcctggat
    11821 gtggtgaatc tgatctccaa agacccgcgt ttccctgatg acctggatgg cgacgggcgt
    11881 cgcttctaca ccgacgggcc acgagcacac gagtttttgc acgagatgaa ccgcgatgtg
    11941 tttacgccac gcaggttaat gaccgtaggt gaaatgtcct ccaccagcct tgagcattgc
    12001 cagcgatatg cgacactgac aggcagtgaa ttgtcgatga cctttaattt tcatcacctg
    12061 aaagtcgatt atcccagtgg tgaaaaatgg acgctggcta aacctgactt tgtggcgttg
    12121 aaaacattgt tccgccactg gcaacaagga atgcacaacg tggcatggaa tgccttgttc
    12181 tggtgtaacc acgatcagcc gcgcattgtt tctcgctttg gtgatgaagg tgaataccgc
    12241 gtgcctgcgg caaaaatgct ggcgatggtg ctgcatggca tgcagggaac gccgtatatc
    12301 taccagggcg aagagattgg catgaccaac ccgcatttca cgcgcattac tgactatcgc
    12361 gacgtggaga gcctcaatat gtttgccgag ctgcgcaacg atggtcgtga tgccgacgag
    12421 ttattggcaa tcctcgccag taaatcccgt gacaacagtc gcacacccat gcaatggagc
    12481 aacggcgata atgccgggtt tacggctggc gaaccgtgga ttggcctggg cgataactat
    12541 caggaaatca acgtagaagc agcgctggcc gatgagtcct cggtgtttta cacctaccaa
    12601 aagttaatcg cactgcgtaa gcaagaagcc gtcctgacat ggggcgatta ccaggatctg
    12661 ctgccaaaca gccctgtatt gtggtgctat cgccgtgagt ggaaggggca aaccttactg
    12721 gtcattgcca accttagccg tgagacccaa ccctggcagc cagggaaaat gctcggcaac
    12781 tggcagcttg tgatgcataa ctacgaagaa gcctcaccac aaccctgtgc catgacttta
    12841 cggccttttg aggctgtctg gtggttacag aagtaagtat ccgctaagcc ccggtaatac
    12901 tggtcattcc ggggttttgc ctttcagcat gttgatttta taatcaaaaa attcattaaa
    12961 aaatttacat aacgctgtag cgcccgtcat ccgtacgctc tgctttttac tttgagctac
    13021 atcaaaaaaa gttcaaacat ccttgatgca aagcactata tatagacttt aaaatgcgtc
    13081 ccgacccaat atgttgtatt aatcgactat aattgctact acaactcccc acgaaaaagg
    13141 tgcggcgttg tggataagcg gatggcgatt gcggaaagcg ccggaaaacg aaacgaaaaa
    13201 accggaaaac acctttccca atttctgtgg ataacctgtt cttaaaaata tggagcgatc
    13261 atgacaccgc atgtgatgaa acgagacggc tgcaaagtgc cgtttaaatc agagcgcatc
    13321 aaagaagcga ttctgcgtgc agctaaagca gcggaagtcg atgatgcaga ttattgcgcc
    13381 actgttgccg cggttgtcag cgagcagatg cagggccgca gccaggtgga tatcaacgag
    13441 atccagaccg ccgttgaaaa ccagcttatg tcgggtccat acaaacaact ggctcgtgct
    13501 tacatcgagt accgtcacga tcgcgacatt gaacgtgaaa aacgcggtcg cctgaatcag
    13561 gagatccgtg gtctggttga gcagaccaac gcctcgttgc tcaacgaaaa cgccaacaaa
    13621 gacagcaagg tgattccaac ccagcgcgac ctgctggccg ggattgtggc taaacactat
    13681 gcacgccagc acctgctgcc gcgtgacgtg gtgcaggcac atgaacgtgg tgatattcac
    13741 tatcacgatc tcgattactc accgttcttc ccgatgttca actgcatgtt gatcgacctg
    13801 aaaggcatgc tgacccaggg ctttaaaatg gggaacgccg agattgaacc gccgaagtcg
    13861 atctctacgg caaccgcggt aactgcgcag attattgccc aggtcgccag ccatatttat
    13921 ggcggcacca ccattaaccg tatcgatgaa gtgctggcac cgtttgtcac cgccagctac
    13981 aacaaacatc gcaagaccgc agaagagtgg aacatcccgg acgccgaagg ctacgctaac
    14041 tctcgtacca tcaaagagtg ctacgatgcc ttccagtcgc tggagtacga agtaaacacc
    14101 ctgcacaccg ccaacggtca aacgccgttt gtaacctttg gttttggtct gggcaccagc
    14161 tgggagtcgc gcctgattca ggaatctatc ctgcgtaacc gtatcgccgg cctcggtaaa
    14221 aaccgtaaaa cggcggtgtt cccgaaactg gtgtttgcga ttcgcgatgg cctgaaccat
    14281 aaaaaaggcg atccgaacta cgacatcaaa cagctggcgc tggagtgcgc aagcaagcgc
    14341 atgtatccgg atatcctgaa ctacgatcag gtagtgaaag tcaccggttc gtttaaaacc
    14401 ccgatgggct gccgcagctt cctcggcgtg tgggaaaatg aaaacggcga gcagatccac
    14461 gatggtcgta acaacctcgg cgtgatcagc ctgaacctgc cgcgtattgc tctggaggca
    14521 aaaggcgatg aagccacctt ctggaagctg ctggatgaac gtctggtgct ggcacgtaag
    14581 gcgttgatga cccgtatcgc tcgtctcgaa ggcgtgaaag cgcgcgtggc cccgatcctc
    14641 tatatggaag gtgcttgtgg cgtgcgtctg aatgctgacg acgacgtttc tgaaatcttc
    14701 aaaaacggtc gtgcgtctat ttcgctgggt tacatcggca tccacgaaac cattaacgcg
    14761 ctgttcggcg gcgagcatgt ttacgacaac gagcagctgc gcgcgaaagg tattgcgatt
    14821 gttgagcgtc tgcgtcaggc ggtggatcag tggaaagaag aaacgggtta tggtttcagt
    14881 ctctacagca cgccaagtga aaacctgtgc gaccgcttct gccgtctcga taccgctgag
    14941 tttggcgtgg tgccgggcgt gaccgataaa ggttactaca ccaacagttt ccaccttgat
    15001 gtggagaaaa aggtgaaccc gtatgacaag atcgactttg aagcgcctta cccgccgctg
    15061 gcgaacggtg gtttcatttg ctacggcgag tatccaaaca ttcagcacaa cctgaaggcg
    15121 ctggaagatg tctgggatta cagctatcag catgtaccgt attacggcac caatacgccg
    15181 atcgatgagt gctacgagtg tggctttacc ggtgagttcg agtgcaccag caaaggcttc
    15241 acttgtccga aatgtggtaa ccatgacgcc tcccgtgtgt cggtgactcg ccgcgtgtgc
    15301 ggatatttag gtagcccgga tgcacgtcca tttaacgccg gtaagcagga agaagttaag
    15361 cgccgcgtta aacatttggg gaatgggcag ataggttaat cttcactctg cgttaagcaa
    15421 atgccggatg cggcgtaaac gccttatccg gcctacattt ggcaacggct gtaggcctga
    15481 taagacgcga cagcgtcgca tcaggcgttg attactggat gcagcctaca cgcggactga
    15541 taagccaggc gaaataatga attatcatca gtactatcct gtcgacatcg tcaacggccc
    15601 cggcactcgc tgcaccctgt ttgtctccgg gtgtgttcat gaatgccccg gttgctataa
    15661 caaaagcacc tggcgggtaa attccggtca gccatttacc aaagcaatgg aagaccagat
    15721 cattaacgat ctgaatgaca ctcgcatcaa acgccagggt atttccctct ccggcggcga
    15781 tccgctgcat ccgcaaaacg tgccggatat tctgaaactg gtacaacgca tccgcgccga
    15841 atgtccgggt aaagacatct gggtgtggac aggctataaa ctcgacgaac tcaacgctgc
    15901 gcaaatgcag gttgttgatt tgattaacgt gctggtcgac ggcaaatttg tgcaggattt
    15961 aaaagaccct tccctgatct ggcgcggcag cagcaatcag gtggtgcatc atttgcgatg
    16021 ataagttccc tgttacctga gtgcaggcaa cagggaaata aggaattaac gatacttctg
    16081 gtgataggcg ttgcgggtcg ttttcagttg ctctgcggca gcctgcgctt cttttacttt
    16141 accttcattt gccagcttca gcgcgtcgtc aatctgaccg accagaatat cgaaaccgtg
    16201 gcggaagtct ttcatttccg ggctgtccgg tgatttgcct tcgagcttcg gcggcgtcgc
    16261 tttttgcgca tccagcgctg cggcgcgcat cttcgttaac gcgtctttga cttgcgccgc
    16321 gttatcggct ttttcgacca cttttaaatt gtcgttgagg gtttccatat tgtcttcaag
    16381 atcagcggca aacgacgccg aactgaatac caacgaggag actgcaagaa tagctaacag
    16441 gcttttacgc attgctcact tcctttttta tttacacttc atccttcaca ctgcctcatc
    16501 gacggctacg ctcattcgcc cgagtcacgt actgcgagta cgctccccgg tcctcattca
    16561 ctttccgtct cgatgcagtg cgaatgattt tgtgtaattt tttagtttat agcgcggcag
    16621 gtcgcgccag ttttttactg cccggcgatt ttcatctccg gcaacagcac agaaccacac
    16681 tgtatattac tgcgtgtttc aatatcgtta ccgacggtga caatattgcg ccacatatct
    16741 tttaaattac ctgcgatggt gatttcgcta accggatact gaatttcgcc attctctacc
    16801 cagaagcccg ctgcaccacg ggaataatca ccggtaatgg cactcacgcc ctggcccatc
    16861 agctccgtca ccaccagccc ggtgcccatc tctttgagca tttgttcgaa gcttagccct
    16921 tgtccggcaa tccgccagtt gtgaataccg cccgcatgtc cggtgctttt cagccccagt
    16981 ttccgcgccg aatagctggt cagcagccac tgagtcagga tgccatcttt aataatatcg
    17041 cgacgctcgg tgcgcacacc ttcgctgtcg aatggcgtag aggccagccc tttcagcaga
    17101 tgcggatgct cttcaatggt cagccagtcc ggcagaattt gtttacccag cgaatccagc
    17161 aggaaggtag atttacggta aaccgatcca cccgctatcg ccccaaccag atgcccaaaa
    17221 agcccggttg ccacttcatt ggcaaaaatg accggcgctt tcatggtgga gagtttacgc
    17281 ggtgacagac gcgataaagt gcggcgagca cagtcggccc cgacccactc tggcgtttgc
    17341 agatcgctca tcgcacgacc aatggtgtag gcgtaatcac gctccatatc gccattttct
    17401 tcggcaatta cacagctgga gagcgaatga cgcgtagagc agtaaccctg caacatgccg
    17461 tggctgttgc caaaaacttt gacaccgtag tggctgttaa agctgccacc ttcggtattg
    17521 gtgatgcgtt tgtccgcctg caatgctgcc tgttctgcgc gggccgccag ttcaatggct
    17581 tcatccgggg aaacttccgc agggtggaac aagtcgagat ccggtgcgtc aaaggccagc
    17641 agctctttgt ctgccacgcc ggcacaggga tctggcgagg tataacgggc aatatctagc
    17701 gccgcctgta cggtgcgggc aatggcctgc gggcttaaat cggtagacga tgcgctacct
    17761 ttgcggttct ggtgataaac agtgatcccc aacgcgccat cgctattgaa ttcaacattc
    17821 tccacttcac cataacgcgt gcttacgcta atgccggtgg tcttgctgac ggcaacttct
    17881 gcaccgtccg atttgcctga ggccaactcc agcgcagtcg aaactgcttc ttccagaatc
    17941 ttgcgctgcg cttcaacttg agagattact ttcattgcaa gtgccataat gtagagagtt
    18001 tctctgaagt ctaacagaga accgtttttc agtgcgcatc ttaactggta acattagcct
    18061 ctttttttaa ggagcctgag atgactaagc agcccgaaga ctggctcgac gacgttcccg
    18121 gtgatgacat cgaagacgaa gacgatgaaa ttatctgggt cagtaaaagt gaaattaaac
    18181 gcgatgccga ggagctaaaa cgccttggcg cggaaattgt tgatctgggg aaaaacgcgc
    18241 tggataagat cccgttagat gcggatctgc gcgcggctat tgagctcgcc cagcgtatta
    18301 agatggaagg tcgccgccgc cagttgcagc tcatcggtaa gatgctgcgc cagcgcgatg
    18361 ttgagcctat tcgtcaggcg ctggataagc tgaaaaaccg ccacaaccag caagtggtgt
    18421 tgttccataa actggaaaac ctgcgcgatc gtctgatcga gcaaggtgat gacgccatcg
    18481 ctgaagtatt gaatctgtgg cccgatgccg atcgtcagca actgcgtact ctgatccgta
    18541 acgcgaagaa agagaaagaa gggaataagc cgccgaaatc cgcgcgccag attttccagt
    18601 atctgcgtga gttagcggaa aacgaagggt aattttcgtt gttgatgtga gtatggtttg
    18661 ccggatgcgg tgcacctgta ggcctgataa gacgcatcaa gcgtcgcatc aggcactatg
    18721 ctcaaatgcc ggatgcggcg tgaacgcctt atccggccta cacacactct atcaggctga
    18781 ggctgtgaat tactgcgcgg cttccgcttt cttcgctaac ccatccagca gtttctgatg
    18841 gatcccacca aaaccgccgt tactcatcac cagaatgtga tcgcccggct gagcggtttt
    18901 caccaccata tctgccagcg tatccacatc gccgctccag tgtgcaggct gaacgcaggc
    18961 ttctgccact tctgccacct gccacggaat atgcgccggt tgcagcagga agacttcatc
    19021 ggcacgacct aatgaaggtg ccagatcgtc tttgcagatc cccattttca tggtgtttga
    19081 gcgcggttcc agcacggcaa taatgcgcgc cgtaccacca actttgccac gcagcgccgc
    19141 aagcgttgcc agaatcgccg tcgggtgatg ggcaaaatcg tcatataccg tcacaccatt
    19201 cgcttcacca cgcaactcca gacggcgacg agcattaata aacgaaccca gcgcgttagc
    19261 ggcatctgcc ggcgctacac caacatggcg agccgccgca atcgccatca ggccattgtg
    19321 catattatgt tcgcctacca gcgaccattt cacttcgccc actttttcgc catccagcaa
    19381 gacttcccat tcggaagcat cggtggtcag ctttttcgcc tgccagtgac cttgctcgcc
    19441 caccagctcc tgctcgctcc agcagcccat cgccatggtc tgtttcaggt tgatgtcatt
    19501 ttccggccag ataatacggc cctgccccgg aacgatacgc accagatggt ggaactgttt
    19561 ctggatcgct ttcaggtcgt caaagatatc ggcgtgatcg aactcaaggt tgttgaggat
    19621 cagcgtacgc gggcagtaat ggacaaattt agagcgtttg tcgaagaagg cgcagtcata
    19681 ctcatccgct tcgataacaa agaagtcgct ttcgcccaga cgcgccgaaa cctcaaagtt
    19741 ccccggcaca ccgccgatca caaatcccgg tttgtaaccg cactgttcca gaatccaggt
    19801 tgccattccc gcggtggtgg ttttgccatg tgtaccggca acggccagca cccagcggtc
    19861 gcgcagcaca aaatcgtgca gccactgcgg acctgacata taagggatgt ttttttccag
    19921 taccgcttcc acacacggat ttccacgggt catggcgtta ccaataatca ccagatccgg
    19981 ctgtggatcg agctggctgg catcgtaacc ctgaatcagt tcaatgcctt gcttctcaag
    20041 taaggtgctc atcggcggat acacattggc gtccgaaccc gttacttcat ggcctaactg
    20101 gcgcgccagc atcgccagac cgcccataaa cgtgccacaa attcctaaaa tatgaatgcg
    20161 catacgtcac tatccttttt taatctggcg ctcattttac gcatatgtcg ggcaagtgag
    20221 aaacgcattt caggataatc cgtaatttgc tggcgcgatt cacctgagcg caacattggg
    20281 attatttgtt aagattgttg cggtcgcttt actccataaa cattgcaggg aaagttttat
    20341 gaaaacgtta ggtgaattta ttgtcgaaaa gcagcacgag ttttctcatg ctaccggtga
    20401 gctcactgct ttgctgtcgg caataaaact gggcgccaag attatccatc gcgatatcaa
    20461 caaagcagga ctggttgata tcctgggtgc cagcggtgct gagaacgtgc agggcgaggt
    20521 tcagcagaaa ctcgacctgt tcgctaatga gaaactgaaa gccgcactga aagcacgcga
    20581 tatcgttgcg ggcattgcct ctgaagaaga agatgagatt gtcgtctttg aaggctgtga
    20641 acacgcaaaa tacgtggtgc tgatggaccc actggatggc tcgtccaaca tcgatgttaa
    20701 cgtctctgtc ggtaccattt tctccatcta ccgccgcgtt acgcctgttg gcacgccggt
    20761 aacggaagaa gatttcctcc agcctggtaa caaacaggtt gcggcaggtt acgtggtata
    20821 cggctcctct actatgctgg tttacaccac cggatgcggt gttcacgcct ttacttatga
    20881 tccttcgctg ggtgttttct gcctgtgcca ggaacggatg cgcttcccgg agaaaggcaa
    20941 aacctactcc atcaacgaag gaaactacat taagtttccg aacggggtga agaagtacat
    21001 taaattctgc caggaagaag ataaatccac caaccgcccg tatacctcac gttatatcgg
    21061 ttcactggtc gcggatttcc accgtaacct gctgaaaggc ggtatttatc tctacccaag
    21121 caccgccagc cacccggacg gtaaactgcg tttactgtat gagtgcaacc cgatggcatt
    21181 cctggcggaa caagcaggcg gtaaagcgag cgatggcaaa gagcgtattc tggatatcat
    21241 cccggaaacc ctgcaccagc gccgttcatt ctttgtcggc aacgaccata tggttgaaga
    21301 tgtcgaacgc tttatccgtg agttcccgga cgcgtaatct cctgcgcctc tccacacgga
    21361 gaggcgtttt tatcgctgcg caatgttgac tcttgtcaca ggcgaactct gacgattctc
    21421 ccacagcacc gttaatccac gctgtaatgc tataaaaatc aacaacaaaa taccgatggc
    21481 gattttcgtc caccatgagc tcagcgtgcc atcaaagttt atgtaagtct gaatcagtcc
    21541 ctgaatcgcc acgccaaaaa gcgtccctaa taccgttcca acgccaccac tcaaaagcgt
    21601 accgccaatt accactgagg cgatagcatc cagttccaca cctacgcccg ccagcgcata
    21661 tccggcctgg gtataaatcg agaagacaat ccccgccagt gtcgccagcc cggtagagag
    21721 catataaatg cgaatagtgg tgctgcgagt ggaaatcccc atcaggttcg ccgacgttgc
    21781 gttgccgcca atggcgtaca cctgattacc aaaacgcgta cgatgcgcga ggaatatgcc
    21841 gataaccacc accgccagca tcagcagccc catcgcactt aagcgaccgc cgccagggat
    21901 tttccacgca aggcttgaga gcgtgtcata aacaggatgg tttatcggga tcgactcttc
    21961 cgaaacgaga tagctgacgc cacgcaaaaa gaacattcct gccaaagtaa tgataaacgc
    22021 cgggatcttc agggcgtcaa tcagaagccc cataaatgcg ccgaaggcac agcccatcac
    22081 caacaccagc ggaaacgcca gcagcggcga gaggccgaaa tcgccaatca cttttgccag
    22141 aaacacgccg gtaaaggcga tcaccgaacc gacggagaga tcgatcccac cggagaggat
    22201 cacaaaggtc atgccaacgg cgattatccc aagaaaggca ttatcggtca ggatattgca
    22261 gatcactctt gtggaagcaa aaccgggaaa ctgggtcaga cagtagatat aacccaacac
    22321 aaagacgccg atggtgatca tcagtggcaa attacgtttt atcacggtta cgtacccctt
    22381 taatcagact gataaagcgt tgcgactgga caatcagcac gcaaagcacc accaccgctt
    22441 tcaccacctg gttcatctcc ggcggaaatc cagaaagcaa aattccggtg ttcattccct
    22501 gaataatcag cgcccccacc accgaaagca gcaggttaaa gcgcccgccc atcagcgatc
    22561 cgccgccaat caccaccgcg agaatggcgt ccagctccag ccataacccg gcgttattgg
    22621 catcggcacc gcgaatatcc gccgccacga taatgcccgc aatcgccgca catagcccgc
    22681 tcaacacgta ggtgagcatg acgatgattc gcgtgtttac cccggcattt tttgccgccc
    22741 gaatattgat accaacggct tcgataaaca tccccagtgc cgttttgcgg gtcaacagcc
    22801 agaacaggag aagcgtcagc accgcaataa tgactggcgt tggcaggaac aacagcgatc
    22861 cactgccgaa ccaggagaga tccggtgagt taaatgtgac gatctgtccg gaggtgatca
    22921 gttgcgccac accgcgcccg gcgaccatca ggatcagggt ggcgacaaac ggctgaattt
    22981 tgaggatcgc taccagtatg ccgttccaca atcctgccag gatgccagtg cccagggcgc
    23041 ttaacaaaac aatcggcagg ctgaatcccg cgaccgtcat cgcagccgtt gtggctccgg
    23101 cgatagccat caccgcccct acggagagat caatcccacc ggtggcgata accagcgtca
    23161 tgccaatcgc cagtagcgca acgggggccg cacggttaag aatgtcgata gggctaccga
    23221 acaaacgccc atcctgaagc accacctgcc agaaatgcgg ggccaccagg ctatcgacca
    23281 gcagcaccag caacagtgcc gccagctgcg gcattcctgt tggccagcga aaacgccttt
    23341 tcggcggcgt agtgtccggg agagattgag gcatcacact attctcctta cgccgcaatg
    23401 gcgttcatga tcgccggaac ggaaagctct gccagcggga tctccgccac ctgtttgcga
    23461 tcgcgcatga taatcacccg gtcggcatag cccaccagct cttccagttc agaggagatc
    23521 accagcagcg ccagaccgtc agcgcacagc gtttcaatca ggcggatgat ttcagcgtgg
    23581 gcaccaacat caatgccgcg ggttggctcg tcgaggatca aaaattgcgg acgagtcagt
    23641 agccaacgtg aaagcaacac tttttgctga ttgccgccag agagaaattc aatcggttgt
    23701 tcagttgaag gtgtgcgaat gccaagctgg cggataaagc gttcggcaat ctcttgctgt
    23761 tctttgcggg aaatcggtcg tagccagccg cgctgggcct ggagagcgag gatgatattt
    23821 tcccgcaccg aggcggcggc gatgatgcca tcggttttcc tgtcttccgg gcagaagccg
    23881 atacccagta ccgaagcctg atgtggcgat cgcagggttt gctgtttgcc tttgatcaac
    23941 gccgtgccgc tgtcagcagg tttgatcccg aagatcactt cggcggtttc ggtacgtccg
    24001 gatcccagca agcctgccag gccaacgatc tcgccggggc gtacttcgag atcaaacggt
    24061 gcgatcgttc cttttttgcc gtaatttttg aacgcggcaa cgggtttgtc gctcagcaat
    24121 gttctcccgg cacgctgaag cgcgtgggtg tccagttcac gccccagcat catttttacc
    24181 agttcgatct gcggtagctc gcgggtttcc cgacagccca caaaactgcc gttacgtaag
    24241 acggtgatcc gatcgctaac ctgataaacc tgatcgagaa aatgagtgac gaagatcagg
    24301 ctgacgccgc gatcgcgaag ctgacgcatc aggccaaaca gtaactctac ttcctgagtg
    24361 tcgagactgg cggtgggttc atcgaggatc agcactttgg cggagagatc gatagcccgg
    24421 caaatggcga cgatttgctg catcgcgact gaaaagcggt tgagcggttc gcgcacgtcg
    24481 agggagaaac cgtaagatgc catcagttcg gtggcgcgct tttccatctc tttacggcgt
    24541 agaaggccga aacgtttggg ttcgcgacct atgaatagat tatcagcgac cgacatattg
    24601 ggtagcaggt tgacttcctg atagacggtg ccgatgccga gttgttgtgc gtgggcggtg
    24661 tttttcggtg agatagcctg gccttccagc cagatagtgc cgcgatcggc gtggtataca
    24721 ccggttaatg ctttgattag cgtcgatttt cccgccccgt tttcaccgag cagcgccatg
    24781 atttcgccac ggcgcaggct gaaatcaacg ttgtctaacg ctttgacacc ggggaaaaat
    24841 ttacttaatc cttcggtgcg gaggatttcc tggtgttggt cggtcgtcat ggtttccccc
    24901 ggtgtaaagc gtgctcccag taggcatgat aagacgcggt aagcgtcgca tcaggcttca
    24961 gcgcacaacc gccggatgcg gcgcgagcgc cttatccggc ctacgaatcg gcatcaggca
    25021 tagcaaccct caatacccca tatttttctt cttctctaac tcttcttttg cggtatcagg
    25081 cagataaagg gtggatttgg ttaacgtcag cttttcaggc atggtgccgt cttttttata
    25141 tttctccagc gcgtcgaagg cgggacctgc catattcggc gtcagttcaa cactggcgtt
    25201 cgcttcgcca tcaatcatcg ctttgtagat gtccggcaca ccgtcgatag agcctgtcag
    25261 gatatctttg cccggtttca ggcccgcttc tttaattgcc tgaattgcac caatcaccat
    25321 gtcatcgtta tgggcgtaaa ccatgcagat gtttttgccg ttgttttccg ctttgataaa
    25381 gctctccatc acttctttgc ctttgctgcg ggtgaagtca cctgactgcg agcggataat
    25441 tttgatattt ggcgcattct taatggcttc ggcaaagcct ttcttacggt caatggcgac
    25501 gctggcccca acggtgccct gcagctccac cacgttgcat ggtttgccat tcacttcttt
    25561 taccagccag tcaccaatca acttgccttc gaggatgttg tcggcggtga cagtggtcat
    25621 atagagagat ttgtctttca catcgatgga acggtcaagc aagaagaccg ggatttcggc
    25681 atctttcgcc tcttttaata ccggctccca accggtcgct accactggag cgataaagat
    25741 cgcatccacc ccttgcgcga cgaaggaacg taccgcttta atctggtttt cctgcttttg
    25801 ctgaccatcg gcaattttca acgtaattcc gcgcttttcg gcttcacttt tcgccacatt
    25861 ggtttctgcg gcgcgccagc cggattccga cccgacctgc gaaaatccaa cggttaatgg
    25921 agcggccaac gccatagacg acatggctgc cgagactgca gagactacaa gtaagcgttt
    25981 ccacataggg ttgtcctcgt cggggtaatt attattggtg aaaagatgtt cgcgaaaaaa
    26041 ctatagacaa ttcgttatgt aacggattgc gttacatcac acttcaaaaa agtaaataaa
    26101 acgttaatca caagtttgta atcgctttca tctcactatg aaaaatgcgg ctacggttat
    26161 ggattttcct gctctgtata ccgtcttaaa actggcgaaa aaggaaaatg aagacgaaaa
    26221 caagcgaaga cattcggcgc gagttggcta taatactcgg cacttgtttg ccacatattt
    26281 ttaaaggaaa cagacatgag cttactcaac gtccctgcgg gtaaagatct gccggaagac
    26341 atctacgttg ttattgagat cccggctaac gcagatccaa tcaaatacga aatcgacaaa
    26401 gagagcggcg cactgttcgt tgaccgcttc atgtccaccg cgatgttcta tccgtgcaac
    26461 tacggttaca tcaaccacac tctgtctctg gacggtgacc cggttgacgt actggtcccg
    26521 actccgtacc cgctgcagcc gggttctgtg atccgctgcc gtccggttgg cgtactgaaa
    26581 atgaccgacg aagccggtga agatgcgaaa ctgattgctg ttccgcacac caagctgagc
    26641 aaagaatacg atcacattaa agacgttaac gatctgccag agctgctgaa agcgcaaatc
    26701 gctcacttct tcgagcacta caaagacctc gaaaaaggca agtgggttaa agttgaaggt
    26761 tgggaaaacg cagaagccgc taaagctgaa atcgttgctt ccttcgagcg cgccaagaat
    26821 aaataagttc ttctggcgca ataaccctga acaccgggct tcggttagta agggtttttt
    26881 tatgcccgca atttataaac tctctactcc actattatta ttttcagcag ttgaaaggct
    26941 tgaacggtaa gaacaagcta aactcgacca ccattttgct gttcatagcc acttgctgga
    27001 agttagccgg cctcactcat actcaccgtt caaagcgtgt ccccgtttta cccctgtaca
    27061 tttgttaacg ccagcagagt tttttatgcg attgcagata tggaaagcgg cccggagaga
    27121 aaagcaaaag atgggaaagt tacaaaaaaa atcgcgagat acatcgcata aaacaacgcc
    27181 acccgaaggt ggcgtgcttt tattattact tatccctgtc taaccagtcg ccgctttcaa
    27241 ttagctttaa tccatcgacg ggtcgttgat aaacgtacat ccatgcactc ccgtacggcg
    27301 tctgaatcaa ctggcgcgcg tattcaccgc ccctggtgcg caaggcatca agttcggcca
    27361 gcgtggcgtt atcaatacga taaacttcac cgtgtactgt tccgttcccc ggaactgcgc
    27421 ctggatagtg gcccaggcta tacaactggt agttatcgat actgaaatcg cccagtaact
    27481 gggcattggt catccagtga ctgttgcctt gtttgtggcg taaactgccg tagacaaata
    27541 ttcgcattgc taaaactcga actgatagag caaatccagt gcctggtcta caccagacac
    27601 ggcttccaga tatagcttag gcatcaggcg ataacgtaac gtgagtgttg ctatagagtc
    27661 aaatataccc acgccgtatt tcacttgcag acctggcaat acatagccgc tgaccaccac
    27721 ctgggaggag tcgcctactc cctgggtgtc gagcgctaaa ttgcttacgc caaacgtctc
    27781 gccgatttta cccacaatct gaccactttg tgcaaccccc agaccaatca gcatcgaggt
    27841 catcgccgca ctgtcgctct gatcgctctc cagcccttgt ccacgtagca aataagacaa
    27901 ggcagcctgt tgcgacatgg ccgggtcaga gaagatctcc gctttcggtt cgtccgccag
    27961 accagtgacg cgaactccgg cgattacgtc gtcttctgta gcatccgggt tacgaatagc
    28021 ttcgatatta agatacggtt gatccggcgg accagagaac agtaactcac ccttacgcac
    28081 aatcagatcc tgaccatagg catggaagcg cccttcaggg atgttgatct gcccgttcag
    28141 gcccagccct tgtttgtcct gaacgacgtt gagatcgccc gtcagccgcg ctttcaggcc
    28201 aaaggcgtca atgcgcacat tgttgccgac gtggacaatc aggttgctgt taatcggaat
    28261 cgacgccgtt tttgcctctt ccggttgcag gttatcgtta agcattacca catcgctgga
    28321 gacgcccacc gcgctttccg gcagctcatg caccacgatg cgcgcccacg ggacatccac
    28381 gcgaccatcg agggtaaaca ggtttggtgt agcctcgaat acaacatctg gcgatacatc
    28441 cattcgtacc atcggcggca cggtgatccg cactttactg cctttcgccg ttacccgcgc
    28501 ccgccagttt tcaatctggc tccagtcggc atcaccgttc aggtagattt caccctgctg
    28561 ggtccgtact gtaccggcaa gcgtcgagcg cataccgtta aagttgaccg caagctggct
    28621 cggttgcata tcaaacggca taaagttgcc gtcgatatcc acacccgtaa cctgaagctg
    28681 accaaacaac tgcgggcttt gcacatcacc acccagacgc aagttggcac tcaccatccc
    28741 cgctgctttt tccccacggg taaagatggg gtttatcatc gcaaggttga agttacggat
    28801 attgacgttg ccaccaagat tacggcggcc ttgcggatcg gtcacctgca cctgtccatc
    28861 aaactggccg ttattggtca ggcggatggt ccagcccaat tcggcacggt tgttacgcaa
    28921 ttccgccgtc agattcagcg tctgaaacgc cacgggcagc gccgcatcgt tgacggtttg
    28981 cgtcacctgc acattacgcc ccgaaagggt gatactgccc tgcggcagcc cctctttcgt
    29041 ggtgtcccag gcaacgtccg ctttacccgt gaagataccg ctggcctgag tggtttctgg
    29101 cataaatggt ttcagcatgg ccaggtcgaa gcggttgaga ttcaccaccg cacgcccttc
    29161 ggcacccgca tcgatagttt gcggcacgca cagttccgca ttcgggttaa gccagcagtg
    29221 tggcccgatg ctgatttttt gctccttatt gcggtaatcc agcgcaatat cgcgggtcag
    29281 cgaccacggg ccgaccggcg tctggaagcg ggtattgcta agagttccct tccagcgttc
    29341 ttctttgcga tcaaaacttc ctgccagatt aagctgcccg gagacaggct cgccctgaat
    29401 ccgcaactgt agctcgtgct gcttttcgct gcctttggca ttcagggtga cgaggttgat
    29461 atttacatcc ggctgcgaaa tttgctcaac gcgtacgtcg agtttacccg caatctggtc
    29521 ggtggatttg atatcgcctt ccacgcgaac ctgcgccacg gaaagttcct gccagcgcag
    29581 gccgcgcgcg gtgatatctg ccagtagttg tggcgcttcc accgtgccgc gtactttcac
    29641 caaccctttc gctgtaccgc caagccccgg cagcgcgtta tccagccccg gcgcgttgat
    29701 ggtggcatca agattgagat cttttacccc cagctcgcct ttcacttcgg cactgtttgg
    29761 ccccagttcc agatgcagcc ctgggatcat ccactgcata taactgttgc ctttcagcgt
    29821 gccgtcaacg ttcactttgt tctgtttgac gttaccggtc agcttcagtt ctggcacctc
    29881 catctgccag gtgccgccgt acaggctacc gcgggtttta atcaagccat tgagtttcga
    29941 cggccactcc gggatctctt tggcggtgtt aataccgtta agcgttagct caccgcgcca
    30001 actaatggcc tgctgccagt cgagcaacgc cttaagttcc gttttccctt ccagcgccgc
    30061 gacggtaagt ttgtcgagat tgacctgctg ttcattacct ttggcatcaa gggtaatggt
    30121 ggctggcggg atctcctggc ccttaacagc cgtgcgcata gagatcgcat aatcggtcat
    30181 tttgccggta agtttcagtt tcagatcatc cgcctgatac tgcttctcac cagtgaacgg
    30241 ccagtaaagc tgtttgctgt tcacttccac gttgagcggc aatccggctt ccgccagtcg
    30301 cgtctgggcg cgtaaatcca tatccaccgg accggaaagg ttaacgccta tctccagctg
    30361 ttcgcgcagc gcaccgccca ctttcagctt caccttttca cctttcaacg gctccacgtt
    30421 cagtgtgctg ttgagggtga tatccaccgg ccagttgtct gacagctgcg ccgtgccgct
    30481 ggcgttgacg atcccctggt tggagtcgat atccagcgcg tccagtttgg tattgccgtc
    30541 gatactgctc actttcagca gcatggtgcg cacggtgatg tccgtgtcgc ccgtcacgcg
    30601 cagttgctcg cccttaaact cttcaatgtt caggttgagc ggcagatgca cgtcggtcat
    30661 ttccggcaat accgggcgag aaaagagatc tttcagcgtt tcgccgagcg gcttttcctc
    30721 cggctgtgga ttttcaattt tcggttcgac cacttcttcc tgcgccgctt ccgccacttt
    30781 cggcaaagca atcaacaggc ctttcagcga tgtcggtttc agggtcaggg ttttctcctg
    30841 ccagttcagg ccggaggtga agtccatcac cgataccgtg gtgtcatcaa tcttgatgtt
    30901 gacgttgtcc agcgccaccc gtgtcagggt aatgggatac ggcatggaga gatccaacgg
    30961 accgctatct tcctcttctt caacctgttc agaaggaggc atttttttac tgtcgatgtt
    31021 gacctgaatg tctttcagcg ccagatcatt aatacaaacg ctactgttcc acaggcactc
    31081 aagcccgacc gccagatgca gattgcccgc ttttaccgcc acgcctggct gctcataacg
    31141 aacgtcagac aaggtgagat cacgccagcc gccggtgacc ttgccaatat ccagtcctgg
    31201 cacccagcga tccgccgctt taaataccag atgcaggccg ctggtggtgc ccaccagaaa
    31261 cgccaccgat cccagcaaca gtaagataac gataaccacg ccgaggctga tttttttcca
    31321 taaactcata attctggccc cagaccgatg taaaactgta acccgtgttc gtctttatcc
    31381 gcgaccggta cggcaaaatc gagtttgatt ggcccgaccg gcgattccca gcgcacgccg
    31441 accccggtac cggttttaaa gtcgctgcgg cgaatatcgc ttaccgcttc gccgctatcg
    31501 acaaacaccg cgccccacca ttttccggtc acgttgtact ggtattccag cgatccggtt
    31561 atcagcttcg aggccccttt caggtcaccg ttggcgtatt tcggggcgat agatttgtat
    31621 ttgtagccgc gaatactgcg atcgcccccg gcgaagaaac gcagatccgg cggtactttg
    31681 tcgaaatcac ccgtttcaat ccagcccagc gtgccgcgtg tcacaaaacg atggcgatcg
    31741 tacagtgtgc ggatccagac gttctgcgcc tggaaaacgg agaaatcaac atccgagccc
    31801 caggccgtgt tggagtagtc gatagagtag cgttgcgagt cgccccaggt tggcatcagg
    31861 ccaccacgag aacgcgtgcg gctaatcatc accccaggat aaaacagcat cgtggtgttg
    31921 gtaatttcac cctgggtaaa gtggtcgaaa ctccagcgca ggttaatggc acgctgccag
    31981 ccgctggaga gatcccagta gcgagaagcc accagcgtag tggagtcaga ctcggtatcg
    32041 ttcaggtcag tgcgcttaaa accgccctgc accaaataat attgttccag cggattcttc
    32101 agcagcggca ttttatagct gaagtcgagg atctgttccg gcgcggaaat actggtactg
    32161 gtggtcaaac tgtgaccgta agagttcatc cacggctttt tccacgtcgc tttcacgcgc
    32221 ggtcccacgt ccgtagagta accgacgccg gtttcgatgg tgttttctgt tcgcggcgaa
    32281 accacgcccg tcaatggtaa tactttcgtt tcgcgcgctt tatcaaattg tggagccacc
    32341 accaccgagt taaaccagcc ggtagcagaa agtcgacggt tcagttctgc cagatctttc
    32401 gattcgtact catcgccctc tttaaacggc accagatttt gcaggtattc atcgcggatt
    32461 tgtgatcctt caaaggtcac atgcccaaag cggtaacgtt cgccactgtt ataatcaata
    32521 tcccagaagg ctttatgcag gccgagcgca atgcccagct gcgctttggt aaattcgcta
    32581 tcgaaataac ctttacgcaa cgcaatgctg gttaaggact ttttgaaatt ttcataatcg
    32641 ccctggttca gtaccgtacc aatagccggg cgagtatcga gcaatttcaa atagtcttta
    32701 tcggtccgcg cgccgccgcg caataccaca tcggtgccgc caattaacac cggcacgcct
    32761 ggcgtgactt tggcgatcaa tacctgccgc cctttctttg gcggtggacg gagatcaaat
    32821 tcaatggtcg gctggtaata gcccagcgct ttcagacctt cgcggatggc atcatcgacg
    32881 cgtgcgcgaa agcgacggtc tggcgtcact tcatcacttt caatcgtaga aagctgcgcg
    32941 cgaacgttct tttccagctg tcccgataac ccctcgacct gtagacggac gttcgcggcg
    33001 acggcagatc cgcttaagca gagtaagctt acacagcata actgtcggat atagcgcaca
    33061 ttttctcctg aatatccttt tttcctgccc ctggaacgcc gttaaacggc ttaacaaaaa
    33121 tccagtaata tggattaaaa agcagactaa acccaaaata tttcttatgt tttactttag
    33181 acctattcac ggtggttatt gtgtgcaaat acgcctcttg ttacaacctt aaccccaatg
    33241 accgattttc gggagagcga caccatgagt ttatttgata aaaagcatct ggtttccccc
    33301 gccgatgccc tgcctggacg taacaccccg atgcccgtag ccacactgca tgcggtcaac
    33361 ggtcactcaa tgaccaatgt acctgacgga atggagattg ccatttttgc gatgggttgt
    33421 ttctggggtg tggagcgtct gttctggcag ttacccggcg tttacagcac cgccgcaggc
    33481 tataccggcg gctatacgcc aaatccgact tatcgggaag tgtgctccgg cgatactggc
    33541 catgccgaag cggtgcgcat tgtttacgat ccatccgtca tcagctatga gcagttgcta
    33601 caagtatttt gggagaatca cgatcccgcc cagggaatgc gtcagggcaa tgaccacggc
    33661 acgcagtatc gttcagcgat ttatccgctg accccagaac aggatgccgc cgctcgcgcc
    33721 agtctggaac gttttcaggc ggcgatgctt gccgccgatg acgatcgtcg catcaccacg
    33781 gaaatcgcta acgccacacc gttttattat gccgaagatg accaccagca atatctgcat
    33841 aaaaacccgt atggttactg tggaattggc ggaattggcg tctgtctgcc gccggaagca
    33901 tagcgttacg ggtacaaatg tagattgttg ataaagtgca ttttgtttat gccggatacg
    33961 gcgtaaacgc cttgccaggc ctacaaaatc gtccaaattc aatatattgc ggggactgcg
    34021 taggcctgtt cggcatagcg catcaggcag tttttcattt ttcagctgtc tgaattttaa
    34081 ccctctggcg gctttacagt accttacgct atactagcca ctgaaaatgc cggttcactt
    34141 tcttcgaatc ggctttcaat gtgtatttca cacaaattaa tcaacttccc cttccgagga
    34201 tctggcctga aaggtcggat aagatatgtt aaacagtatt ttagtcatac tctgtttgat
    34261 cgctgtaagt gcgttcttct cgatgtccga gatctcgctt gccgcctcac gcaaaatcaa
    34321 acttaaactg ctggctgatg aaggcaatat aaatgcccaa cgcgttctga atatgcagga
    34381 aaatcccggc atgttcttta ccgtggtcca gatcggtctg aacgcagtgg cgattctcgg
    34441 cggtatcgtc ggtgatgcgg cattttctcc agcttttcac agcctgttct cccgctatat
    34501 gtcggcagaa ctctctgagc aactgagctt tattctctct ttctcgttag tgactggcat
    34561 gtttatcctg tttgcggatt taaccccgaa acgcatcggt atgattgcgc cagaagctgt
    34621 ggctttgcgt atcatcaacc cgatgcgctt ctgcctgtac gtttgcaccc cgctggtgtg
    34681 gttcttcaac ggactggcga acataatctt ccgtattttc aaactgccaa tggtacgtaa
    34741 agatgatatc acttctgatg acatctacgc ggtagtggaa gccggtgcgc tggcgggcgt
    34801 gttacgtaaa caggaacacg agctgattga aaacgtcttt gagctggaat cccgtaccgt
    34861 tccgtcttca atgacaccgc gtgaaaacgt gatttggttt gatctccacg aagatgagca
    34921 aagtctgaag aataaggtgg cggaacatcc gcactctaag tttctcgtct gtaatgaaga
    34981 tattgaccac atcatcggct atgtcgattc taaagacctg ctgaaccgcg tgctggctaa
    35041 ccaaagcctg gcactgaaca gcggcgtaca aattcgcaat acgctgattg tgccggatac
    35101 gttaaccctt tcagaggcgt tggaaagttt taaaaccgca ggtgaagact tcgcggtgat
    35161 catgaacgag tacgcgctgg tagtggggat catcaccctc aacgacgtga tgaccacgct
    35221 gatgggcgat ctggtcggtc aggggctgga agagcaaatt gtcgcccgtg atgagaactc
    35281 atggctgata gacggcggca cgcctattga cgacgtcatg cgcgtgctgg atattgacga
    35341 gttcccgcag tcgggcaact acgaaaccat cggcggcttt atgatgttta tgctgcgtaa
    35401 gatcccgaaa cgcaccgatt cggtgaaatt cgccggctac aaatttgaag tggtggatat
    35461 cgataactac cgcatcgacc agctgctggt gacccgaatc gacagcaagg ccaccgccct
    35521 ttcgccaaaa ttgcctgacg ctaaagataa agaagaaagc gtcgcgtaac ccccagaaac
    35581 atcaacggct cctgaatcag gagccgtctt attactgcat agcactttgg ttaagccatc
    35641 tctgtttgca gacgcataac ctgacggtta acttcggaca tcactgacaa atgcagctta
    35701 tccttcactt ttgggataag aatcttaccc ttatcaaatt caaaagcgcc aacgtccttg
    35761 atgtataacc gtccacggaa caggatcttt acgtacttcg ccacctgaag tgggttgtac
    35821 cgttggaaaa ttttcattgt tatctcctgc tgagtattac gcctgtgcgg tgccacaatc
    35881 ggcccaacta ttatgaggcg caaattttaa tgcttagtga ctatagacta tccgggcaat
    35941 gtttccaccg cgtataactt tttttaccta aaggttacaa ttattcagaa ttatcttttt
    36001 accgaagcgc cgtcttcagt ataagcattc atttttcata tgatttgtgc gcttgaccgc
    36061 aaactggcat cacacttgcg ggaaattcga taaatagcac atatgattaa aactcagacc
    36121 caagtggtcg gatcacctgc atatcataag aaggaaacac catgacccta cgcaagattc
    36181 tggcactcac ctgcctgctg ttgccgatga tggcttccgc acatcagttc gaaaccggtc
    36241 agcgagtgcc gccgattggc attaccgatc gtggcgagtt ggtgcttgat aaagatcagt
    36301 ttagctacaa aacctggaac agcgcgcagc tagtgggaaa agtgcgagta ctgcaacata
    36361 ttgctggtcg cacctctgca aaagagaaaa acgcgacgct gattgaagcg attaaatcag
    36421 caaagttacc gcacgatcgt taccagacca ccaccattgt taacaccgac gacgcaattc
    36481 cgggttccgg catgtttgtg cgcagcagtc tggagagtaa taaaaagctt tatccctggt
    36541 cgcagtttat tgtcgatagc aatggcgtcg cgcgcggtgc ctggcagctg gatgaagaaa
    36601 gttccgctgt agtggtgctg gataaagacg gtcgcgtgca atgggccaaa gacggggcgc
    36661 ttactcagga agaggtgcag caagtgatgg acctgctgca taaattaatt aataaataga
    36721 tactctgaac cccggattca ggaacgactc gcgcggagtg taatccagcg gtttaccctg
    36781 ccagtcgtga acgtgcgctc cggcagctac agccacggca tgaccagcgg cggtgtccca
    36841 aatattcgtt ggtccgaagc gcgggtacag ctgcgcctgc ccttccgcca ccaggcagaa
    36901 tttcagcgaa gagccgatgg acgtggtctg atgttcgcca agctgttgca gatactcttt
    36961 cagctctgca tctgcatggg atcggctaat aacgaccagt ggcgggcgcg catcacggac
    37021 ctgaatctgc ttgcgcacgc cgcactcttc tttccacgct ttgccttctg ccgcgctgta
    37081 cattacgttc attaccggcg catacaccac acctaaaatc ggtttgccgt ggtcgatgag
    37141 cgcaatgtta acggtgaatt cgccattacg tttaataaac tctttagtac catccagtgg
    37201 gtctaccagc cagtaacgct gccagtgctg gcggacttcc caaccgggag gatcttcttc
    37261 agaaagcacc ggaatatccg gtgtcagcgt acgtaaaccg tccatgataa cggtgtgtgc
    37321 ggcaatatcc gctgccgtta ccggagaatt gtccgctttg ctgacgacgt ccatcggttt
    37381 cgtcccgtcg tagacctgca taatggcatc gcctgcattc cgtgcaagct ggcatacttg
    37441 atctaacatt tctccacctc gtctctgtga gcggtgttaa cttattgttt tacttatacc
    37501 ctatcgttaa tgaatgcgcc aactgtgata gtgtcatcat tttcaaagcg taaaattgtg
    37561 gcattcttca ctgttctata agtaagacgt ttattctccc ttttctttcg tattcccgat
    37621 gataaaagga tgtccctgat gattaagttt agcgcaacgc tcctggccac gctgattgcc
    37681 gccagtgtga atgcagcgac ggtcgatctg cgtatcatgg aaaccactga tctgcatagc
    37741 aacatgatgg atttcgatta ttacaaagac accgccacgg aaaaattcgg actggtacgt
    37801 accgcaagcc tgattaacga tgcccgcaat gaagtgaaaa acagcgtact ggtcgataac
    37861 ggcgatttga ttcaggggag tccgcttgca gattacatgt cggcgaaagg tttaaaagca
    37921 ggtgatattc acccggtcta taaggcatta aatacgctgg actataccgt cggcacgctt
    37981 ggcaaccacg agtttaacta cggtctggat tatctgaaaa atgcgctggc gggagcgaaa
    38041 ttcccttatg taaatgccaa cgtcattgac gccagaacca aacagccaat gtttacaccg
    38101 tatttaatta aagacaccga agtggtcgat aaagacggaa aaaaacagac gctgaagatt
    38161 ggttatattg gcgtcgtccc gccacaaatc atgggctggg ataaagctaa tttatccggg
    38221 aaagtgacgg tgaatgatat taccgaaacc gtgcgcaaat acgtgcctga aatgcgcgag
    38281 aaaggtgccg atgtcgttgt cgttctggcg cattccgggc tgtctgccga tccgtataaa
    38341 gtgatggcgg aaaactcagt ttattacctc agtgaaattc cgggcgttaa cgccattatg
    38401 tttggccatg ctcacgccgt tttcccaggt aaagattttg ctgatatcga aggggctgat
    38461 atcgcgaaag gcacgctgaa tggtgttccg gcggtaatgc caggcatgtg gggcgatcat
    38521 cttggtgtgg tcgacttaca actcagtaat gacagcggta aatggcaggt gacgcaggcg
    38581 aaagcggaag cacggccgat ttacgacatc gcgaataaaa aatccctcgc ggcggaagac
    38641 agcaagctgg tagaaacact caaagccgat cacgatgcca cacgccagtt cgtcagcaag
    38701 ccaatcggta aatctgccga caatatgtat agctatctgg cgcttgtgca ggacgatccg
    38761 accgtgcagg tggtgaacaa cgcgcaaaaa gcgtatgtcg agcattacat tcagggcgat
    38821 ccggatctgg caaaactgcc agtgctttca gctgccgcac cgtttaaagt tggtggtcgc
    38881 aaaaatgacc cggcaagcta tgtggaggtg gaaaaaggcc agttgacctt ccgtaatgcc
    38941 gccgatcttt atctctaccc caatacgctg attgtggtga aagccagcgg taaagaggtg
    39001 aaagagtggc tggagtgctc cgccggacag tttaaccaga ttgatcctaa cagcacgaaa
    39061 ccacagtcac tcatcaactg ggatggtttc cgcacctata actttgatgt tattgatggt
    39121 gtgaattatc agattgatgt tacccagccc gcccgttatg acggcgagtg ccagatgatt
    39181 aatgccaatg cggaaaggat taagaacctg acctttaacg gcaagccgat tgatccgaac
    39241 gccatgttcc tcgttgccac caataactat cgcgcttacg gcggcaaatt tgccggtacg
    39301 ggcgacagcc atatcgcttt tgcttcaccg gatgagaacc gctcggtgct ggcagcgtgg
    39361 attgctgatg agtcgaaacg tgcgggggaa attcacccgg cggcagataa caactggcgt
    39421 ttagcaccaa tagctggcga taagaaactg gatatccgtt tcgaaacctc tccgtcagat
    39481 aaagccgcag tgtttattaa agagaaaggg cagtatccga tgaataaagt cgcgaccgat
    39541 gatatcgggt ttgcgattta tcaggtggat ctgagtaagt aaaacacttc tttttcggcc
    39601 tacaaatcat caaccgcatc cggcatttat tggcggatgc gatgatgacg catcttatcc
    39661 gccctacaag ccatgaaccg tagtccggat aagcgcagcg catccggcag ttatgccgca
    39721 cgttcatccc gcaccgctaa cacctcgggc aaattcaact caatccagtc cgccagtgca
    39781 gcaacctttt cgctcacctg ctcgcccagc ggcgtgaggc tatattccac atgcggcggc
    39841 accaccggat acgcgatacg gttaagaaaa ccatcctgtt ctaatgcctg taacgactgc
    39901 gcaagcatct tttcactcac cccgcccatt ttgcgccgca ggtcgctaaa gcgatgagta
    39961 ccttcgcgta gcgccaccag aatcaacacc ccccaacggc tggtgacgtg tttcaaaacc
    40021 tcgcgcgacg gacactgttc agcgaagaga tttccctctt tcagttgttg cgacaggcta
    40081 acctgactca tctcatactt acctttttgt acgtacttac taaaagtaag tttaagtgtt
    40141 agcgtattta aacacaagac aaaacgatgg agacttccca tgatcgctat tactggtgcc
    40201 actggccaac ttggtcacta tgttattgaa tccttgatga aaacggttcc tgccagccaa
    40261 atagtggcta tcgttcgtaa tccggcaaaa gcacaggcac tgacagcaca aggcattacc
    40321 gtgcgtcagg ctgattacgg cgatgaagcc gcactgacat ctgcacttca gggagtggaa
    40381 aaactactgc tgatctcttc cagcgaagtg ggtcaacgtg ccccgcagca tcgtaatgtt
    40441 attaatgccg caaaggcggc cggcgtgaaa tttatcgctt ataccagcct gctacatgcc
    40501 gattcctccc cgctcggcct cgccgatgag cacatcgaga cggagaaaat gctggctgat
    40561 tctggcatcg tttacaccct gctgcgcaac ggctggtaca ccgaaaacta cctcgccagc
    40621 gccccggcag cactggagca cggcgtattt atcggtgccg caggcgatgg caaaattgcc
    40681 tcagcaacgc gggctgatta tgcggtagct gcggcacgcg tgattagcga agccggtcac
    40741 gaaggcaagg tttacgaact ggcgggcgat agtgcctgga cgctgacaca gttagcggca
    40801 gaactcacca aacagagcgg caaacaggtt acctatcaaa acctgagcga agctgatttc
    40861 gctgcggcac tgaaaagcgt cggactgcct gatggactgg cggatatgct ggcggattct
    40921 gacgttggcg catcgaaagg cggtctgttt gatgacagca aaacgcttag caaattgatt
    40981 ggccgcccaa cgacaacgtt agccgaaagc gtaagccatc tttttaatgt taataactag
    41041 ttaattaaag tggcatcctc ccgcatcctc tctgataatg acgggatgcc gggagcaatc
    41101 atgtctgctt cctgaacttt cttctgacag accaatggat gccagtaatg attagcggcg
    41161 tgctgtacgc cctgttagca gggttgatgt gggggcttat ttttgtcggg ccgttgatcg
    41221 tgccggaata cccggcgatg ttgcagtcga tggggcgtta tctggcgtta gggttaattg
    41281 cgctgcccat tgcctggctg ggacgcgtgc gtctgcgtca gttggcgcgt cgcgactggc
    41341 ttaccgcctt gatgctcacc atgatgggca acctcatcta ttacttctgc cttgccagtg
    41401 ccattcaacg tactggcgcg cctgtttcca cgatgattat tggcaccctg ccggtggtga
    41461 ttcccgtttt tgccaatctg ctttatagcc agcgcgacgg caaactcgcg tggggaaaac
    41521 tcgccccggc actggtttgt attggcatcg gcctggcgtg tgtgaatatt gctgagttaa
    41581 accacggact ccccgatttt gactgggcac gttatacctc tggcatcgtg ctggcgttag
    41641 tttccgtggt ctgctgggca tggtatgccc tgcgcaacgc ccgctggctg cgggagaatc
    41701 ccgacaaaca tccgatgatg tgggcgacgg cgcaggcgct ggtcacgctg ccggtttctc
    41761 tcatcggcta tctcgtcgcc tgttactggc tgaatacgca aacgccgggc ttctccctac
    41821 cctttggccc ccgtccgctg gtgtttatta gtctgatggt tgcgatagcc gtgctttgct
    41881 catgggttgg cgcactctgc tggaacgtcg ccagccagcg attaccgaca gtgattctcg
    41941 ggccgctgat tgttttcgaa acgctggcag gcttgctgta cacctttttg ttacgccagc
    42001 aaatgccgcc gctaatgacg ctaagcggta tcgcgctgtt agtggttggc gtggtcattg
    42061 cagtcagagc aaaaccagaa aaacctttaa ctgaatctgt ctcagaaagt tgacacgctg
    42121 gcagtgagtt aaataagcct ctgctacgta agggttatag catttgccat aaagatgcat
    42181 ttaaaataca tcttatctta ttaagaatga ggtatcagct atggcttatc gcgaccaacc
    42241 tttaggtgaa ctggcgctct ctattcctcg cgcttcggct ctgtttcgta aatatgatat
    42301 ggattactgc tgtggcggta agcagacgct ggcgcgcgcg gcggcacgta aagaactgga
    42361 tgttgacgtc attgaagccg aactggcaaa gcttgctgaa caaccgattg agaaagactg
    42421 gcgtagcgcc ccgctggcag aaatcatcga ccatatcatc gtgcgctacc acgatcgcca
    42481 ccgcgagcaa ctgccggagc tgatcctgca agcgactaaa gtcgagcgcg ttcacgccga
    42541 caaaccaagt gtgccaaaag ggctgacaaa atacctgact atgctgcatg aagagctttc
    42601 cagccacatg atgaaagaag agcaaattct cttcccgatg attaaacaag gcatgggcag
    42661 ccaggcaatg gggccaatca gcgtaatgga aagcgagcac gatgaagcgg gcgaactgct
    42721 ggaagtgatc aaacacacca ccaataacgt cacaccgccg ccggaagcct gcaccacctg
    42781 gaaagcgatg tataacggca ttaatgaact gattgatgac ctgatggagc acatcagtct
    42841 ggaaaacaat gtactattcc cgcgcgcgct ggcgggtgag tgatctaaaa gctggatggc
    42901 attgcgccat ccagcatgat aatgcgatta tttccgcagt tcagcagccc gcttcttacc
    42961 aataaacagc cagcccagcc ccagcgcgat aaaccacagc ggggtaacca gcagcgcctg
    43021 gcgggtgtca tcttccagtg tcaacaacac cagaacaaac acaaagaacg ccatacatac
    43081 ccagcacatc agcttgccga gtggcatctt gtagatcgac ttctcatgca gatgaggacg
    43141 ctgtttgcga tacaccaggt acgagcaaag gataatcgtc cagacaaaca taaacagaat
    43201 cgcggaaacg gttgtaatca tcgtgaacgc gccaatcacg ctgggattca catacaacat
    43261 caccacgccg ccgagcagac agatacaaga gaaggtcagc cctttcgcgg gtactgcgcg
    43321 cttagagagt ttagcgaacg ctttcggtgc cacaccttcc tgggccagac caaacagcat
    43381 acggctggta gagaagacac cgctgttagc ggaagacgcc gcagaggtca gcaccacaaa
    43441 gttgatcacg ctggcagccg caggcaaacc taccaacaca aacagttcaa cgaacgggct
    43501 tttctccggg actaccgaac tccacggcgt cacggacata atcacaatca gcgcgaagac
    43561 gtagaacata atgatacgga tcggaatgga gttaatcgcg cgtggcagtg atttctctgg
    43621 atctttggtt tccgcagctg ttgtacctac cagctcaatc cccacgaaag cgaaaaccgc
    43681 tatctggaat ccggcaaaga agccacttaa acctttcggg aaccagccgc catcattcca
    43741 caaatgcgcg aatgacgctt ccacaccggt cggtgactga aagtgcatcg ccaccatgac
    43801 caggccgacg actatcagcg acacaatggc gacgattttg atcatcgcaa accagaactc
    43861 catctcaccg aacattttca cggtggcgag gttaagcgtc agcagcagca ctatcaccgc
    43921 cagcgaggcg acccagtcgg agagatcggg gaaccagaac tgagcataag ccgtgatcgc
    43981 caccacgtct gccataccgg ttacaaccca gcagaaccag taggtccagc cggtgaaata
    44041 tcctgcccac ggcccgagta aatcggaagc gaagtcacta aaagatttgt attccagatt
    44101 cgaaagcagc aattccccca ttgcccgcat cacgaaaaag agcataaaac cgatgatcat
    44161 ataaacgaaa atgatcgacg gcccggcaag gctaatcgtt ttgccggacc ccataaacaa
    44221 ccctgtacca atggcaccgc caatggcaat aagctgaata tgtcggtttg tgagattgcg
    44281 ccgtagcgac tgttcagccg gagcctgatc atcggcaacg acttttacct gatctaccat
    44341 gtttttttct tcctgtacct gtctgtgttg ttcaggctct acggccttta gtcagtctat
    44401 gatacgacga atctgtatcg ggactcatcg atattaggta acaatacgcg gtatgaatac
    44461 taagatttag atctaatgtt aattttatgt ttaaagtgag tgtcatatca cggtgataac
    44521 gcgaaacagc tcacaaaaat acacgcttac ggggtctttg caacacaaaa tagcgccgtt
    44581 tggcaaactg aagggtttat tgctgaatgt ctgctcccct ctggttcgag gggaacagaa
    44641 tgcgctgctt agaggatttc cagcagttcg acttcaaaca ccagggtgct gaacggaggg
    44701 atggacgcgc ctgcgccacg ctcgccatat gccagttcct gcgggatagt cagttcccat
    44761 ttagaaccta ccggcatcag agtcagtgct tcaatccagc cagggatcac gccgttaacc
    44821 gggaattcag ctggctcgcc gcgagcaacg gagctgtcaa acacggtgcc gtcgatcagt
    44881 tttccggtgt aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc ttcgccctgg
    44941 ttgatcacgc ggaattgcag gccagattcg gtgctattta caccttcttt tttggcgttt
    45001 tcttccaggt atttcacacc ttcagcagcc atcgcctgga aacgctgacg acgaacggca
    45061 tcggcgcgtt cgtggatttc acgcagcgcg cggtgcacca catcaaccgg aacagccgga
    45121 tgtttgcctt ccagcgcatc ggcaatgcct gcaaccagtg cttctggcag cagcccttcc
    45181 aggccagatt cactcagttg ttgccctacc tgcaaaccaa tgccgtagct tgcttgcgct
    45241 tcgatggtgt caaaagttgg ggtggtcatc gtatttcctt tcttagggta taaagtagcg
    45301 ggcagcatat cagccacact tctttgggta aaactttgtc gcgggaatga tgacaaatcc
    45361 cggggaaaaa gaaacaatag caatatcccg tatgaatata ataaaggcgg gtttaccacg
    45421 gacatcatag cgttagctta ttcataggct atgattgagg aacaagacgc ggagcaggag
    45481 gaaaccatgc ccgggcgctt tgaactaaaa ccaaccctgg agaaagtctg gcacgcgccg
    45541 gacaattttc gcttcatgga cccgctgccg ccgatgcatc gtcgggggat cattattgcc
    45601 gccattgtat tggtagtcgg atttctgctt ccatctgatg atacgcccaa cgcgccggtg
    45661 gtgacgcgtg aagcgcagtt agatattcag tcgcaatcac aaccaccgac agaagaacag
    45721 ctgcgggcgc aattagtcac gccacaaaat gatcctgatc aggtggctcc ggtcgctcca
    45781 gaaccaatac aggaaggcca gccagaagag cagccgcaaa ccacccatac gcaaccattc
    45841 cagccagata gcggaattga taatcaatgg cgctcctatc gtgtcgaacc cggtaaaacg
    45901 atggcgcagc tgttccgcga ccacggactg cccgctaccg atgtctatgc catggcgcag
    45961 gtagaaggcg cggataagcc actgagtaat ttgcaaaacg ggcagatggt gaaaatccgc
    46021 cagaacgcca gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc
    46081 ctgtttaccc gccagccgga tggcagtttt attcgcgcgc ggtagatgaa gggtcaacca
    46141 aaagccagct aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctggggtg
    46201 aaaccatact gatgtgataa accagaccaa gcgtatgctg gttgatcaaa taatatcccc
    46261 caagcgcagc aatattaata ttcacctgca atgggtcgat atctggctta aaaatattgt
    46321 ttttcttccc ttcatccaat aaggattcca ttaactgcaa gtgcgcatgg ttaatttcta
    46381 aaagacgctg tgactttgca taat
//
LOCUS       DAZUCC010000011        45924 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZUCC010000011 DAZUCC010000000
VERSION     DAZUCC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.011"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..261)
                     /gene="yfhL"
                     /locus_tag="ACHY09_000534"
     CDS             complement(1..261)
                     /gene="yfhL"
                     /locus_tag="ACHY09_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFS3883551.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(317..1165)
                     /locus_tag="ACHY09_000535"
     CDS             complement(317..1165)
                     /locus_tag="ACHY09_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS3883552.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            1374..2009
                     /gene="yfhb"
                     /locus_tag="ACHY09_000536"
     CDS             1374..2009
                     /gene="yfhb"
                     /locus_tag="ACHY09_000536"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFS3883553.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2067..2570
                     /gene="tadA"
                     /locus_tag="ACHY09_000537"
     CDS             2067..2570
                     /gene="tadA"
                     /locus_tag="ACHY09_000537"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS3883554.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2567..4123)
                     /gene="mltF"
                     /locus_tag="ACHY09_000538"
     CDS             complement(2567..4123)
                     /gene="mltF"
                     /locus_tag="ACHY09_000538"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFS3883555.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            4381..8268
                     /gene="purL"
                     /locus_tag="ACHY09_000539"
                     /gene_synonym="purI"
     CDS             4381..8268
                     /gene="purL"
                     /locus_tag="ACHY09_000539"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFS3883556.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYVEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGASGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            8825..10252
                     /gene="qseE"
                     /locus_tag="ACHY09_000540"
     CDS             8825..10252
                     /gene="qseE"
                     /locus_tag="ACHY09_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFS3883557.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIDLPSSKNTK"
     gene            10417..11130
                     /gene="qseG"
                     /locus_tag="ACHY09_000541"
     CDS             10417..11130
                     /gene="qseG"
                     /locus_tag="ACHY09_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFS3883558.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            11120..12454
                     /gene="glrR"
                     /locus_tag="ACHY09_000542"
     CDS             11120..12454
                     /gene="glrR"
                     /locus_tag="ACHY09_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFS3883559.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            12515..12853
                     /gene="glnB"
                     /locus_tag="ACHY09_000543"
     CDS             12515..12853
                     /gene="glnB"
                     /locus_tag="ACHY09_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFS3883560.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(12898..14088)
                     /gene="hmpA"
                     /locus_tag="ACHY09_000544"
     CDS             complement(12898..14088)
                     /gene="hmpA"
                     /locus_tag="ACHY09_000544"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFS3883561.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGLSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            14417..15670
                     /gene="glyA"
                     /locus_tag="ACHY09_000545"
     CDS             14417..15670
                     /gene="glyA"
                     /locus_tag="ACHY09_000545"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS3883562.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDASGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(15733..16926)
                     /locus_tag="ACHY09_000546"
     CDS             complement(15733..16926)
                     /locus_tag="ACHY09_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS3883563.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGCLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATKIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            17044..20325
                     /locus_tag="ACHY09_000547"
     CDS             17044..20325
                     /locus_tag="ACHY09_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFS3883564.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNITLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVTKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKCSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            20422..21405
                     /locus_tag="ACHY09_000548"
     CDS             20422..21405
                     /locus_tag="ACHY09_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS3883565.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            21428..22939
                     /locus_tag="ACHY09_000549"
     CDS             21428..22939
                     /locus_tag="ACHY09_000549"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFS3883566.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVSPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKDAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            22964..23962
                     /locus_tag="ACHY09_000550"
     CDS             22964..23962
                     /locus_tag="ACHY09_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3883567.1"
                     /translation="MSASSLLLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            23995..25089
                     /locus_tag="ACHY09_000551"
     CDS             23995..25089
                     /locus_tag="ACHY09_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFS3883568.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            25101..25973
                     /locus_tag="ACHY09_000552"
     CDS             25101..25973
                     /locus_tag="ACHY09_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFS3883569.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKNGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRNDDILC
                     LVYEHRSGVYHYRVSQTFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(26122..26544)
                     /locus_tag="ACHY09_000553"
     CDS             complement(26122..26544)
                     /locus_tag="ACHY09_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFS3883570.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(26641..27843)
                     /gene="hcaD"
                     /locus_tag="ACHY09_000554"
     CDS             complement(26641..27843)
                     /gene="hcaD"
                     /locus_tag="ACHY09_000554"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFS3883571.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFSGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAASVMGRNAPPPVQRYLLPRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRIDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     HGDDWICRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLID
                     EDIALKSL"
     gene            complement(27853..28665)
                     /gene="hcaB"
                     /locus_tag="ACHY09_000555"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(27853..28665)
                     /gene="hcaB"
                     /locus_tag="ACHY09_000555"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFS3883572.1"
                     /translation="MSDLHNESIFITGGGSGLGLALAERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVNQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(28662..28982)
                     /locus_tag="ACHY09_000556"
     CDS             complement(28662..28982)
                     /locus_tag="ACHY09_000556"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFS3883573.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(28982..29500)
                     /gene="hcaF"
                     /locus_tag="ACHY09_000557"
     CDS             complement(28982..29500)
                     /gene="hcaF"
                     /locus_tag="ACHY09_000557"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFS3883574.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            complement(29497..30858)
                     /gene="hcaE"
                     /locus_tag="ACHY09_000558"
     CDS             complement(29497..30858)
                     /gene="hcaE"
                     /locus_tag="ACHY09_000558"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFS3883575.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKHGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            30994..31884
                     /gene="hcaR"
                     /locus_tag="ACHY09_000559"
     CDS             30994..31884
                     /gene="hcaR"
                     /locus_tag="ACHY09_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFS3883576.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            32044..33183
                     /locus_tag="ACHY09_000560"
     CDS             32044..33183
                     /locus_tag="ACHY09_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFS3883577.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSSLTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(33175..34455)
                     /gene="csiE"
                     /locus_tag="ACHY09_000561"
     CDS             complement(33175..34455)
                     /gene="csiE"
                     /locus_tag="ACHY09_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFS3883578.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRKSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(34646..35500)
                     /locus_tag="ACHY09_000562"
     CDS             complement(34646..35500)
                     /locus_tag="ACHY09_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3883579.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAEKAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(35645..36448)
                     /gene="suhB"
                     /locus_tag="ACHY09_000563"
     CDS             complement(35645..36448)
                     /gene="suhB"
                     /locus_tag="ACHY09_000563"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFS3883580.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            36567..37307
                     /gene="trmJ"
                     /locus_tag="ACHY09_000564"
     CDS             36567..37307
                     /gene="trmJ"
                     /locus_tag="ACHY09_000564"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFS3883581.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            37577..38065
                     /gene="iscR"
                     /locus_tag="ACHY09_000565"
     CDS             37577..38065
                     /gene="iscR"
                     /locus_tag="ACHY09_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFS3883582.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            38177..39391
                     /gene="iscS"
                     /locus_tag="ACHY09_000566"
     CDS             38177..39391
                     /gene="iscS"
                     /locus_tag="ACHY09_000566"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS3883583.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            39419..39805
                     /gene="iscU"
                     /locus_tag="ACHY09_000567"
     CDS             39419..39805
                     /gene="iscU"
                     /locus_tag="ACHY09_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFS3883584.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            39822..40145
                     /gene="iscA"
                     /locus_tag="ACHY09_000568"
     CDS             39822..40145
                     /gene="iscA"
                     /locus_tag="ACHY09_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFS3883585.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            40241..40756
                     /gene="hscB"
                     /locus_tag="ACHY09_000569"
     CDS             40241..40756
                     /gene="hscB"
                     /locus_tag="ACHY09_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFS3883586.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            40773..42623
                     /gene="hscA"
                     /locus_tag="ACHY09_000570"
     CDS             40773..42623
                     /gene="hscA"
                     /locus_tag="ACHY09_000570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFS3883587.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            42625..42960
                     /gene="fdx"
                     /locus_tag="ACHY09_000571"
     CDS             42625..42960
                     /gene="fdx"
                     /locus_tag="ACHY09_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFS3883588.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            42972..43172
                     /gene="iscX"
                     /locus_tag="ACHY09_000572"
     CDS             42972..43172
                     /gene="iscX"
                     /locus_tag="ACHY09_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFS3883589.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            43231..44514
                     /gene="pepB"
                     /locus_tag="ACHY09_000573"
     CDS             43231..44514
                     /gene="pepB"
                     /locus_tag="ACHY09_000573"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFS3883590.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAARLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            44633..45409
                     /gene="sseB"
                     /locus_tag="ACHY09_000574"
     CDS             44633..45409
                     /gene="sseB"
                     /locus_tag="ACHY09_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFS3883591.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(45441..45566)
                     /gene="timP"
                     /locus_tag="ACHY09_000575"
     CDS             complement(45441..45566)
                     /gene="timP"
                     /locus_tag="ACHY09_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFS3883592.1"
                     /translation="MKIRCFCIVLIVSGALLSEVNNNRSLSGNNLLAVNNLQSSK"
BASE COUNT        10770 a        11627 c        12668 g        10859 t
ORIGIN      
        1 ttaaatttta tccgcgtgat gcataagcac aaatttatcc cacaactgtt cttccgtctc
       61 gacatgttgc ggatccttca caatagtatt ggggatcggg cacaccttct ggcaggttgg
      121 tgtctcgtag tgccctacgc attcggtaca cttatcgctg ttaatctcgt agatatgatc
      181 gcccattgaa atcgcctcat tcgggcattc gggttcacac atatcacaat tgatgcattt
      241 tttagtgatt aacagcgcca taaggaaact ctcaaaacaa cacaaaccgg gcgggcatta
      301 tacgcgcatt ctttactcag accagttttt tcaccagcgc ttcactgtga cgaatacgtt
      361 ctggagccag ttccagatcc tgctgaatca gcgcaatgaa cagcaaatcc gttaacattc
      421 cctgcgcgtg acaagcagag attgaagcac tgtttgtcgc ttgttcttcg gcaatggtat
      481 acaggcaatg agaagcacgc tgttgcaggg cattgggagt aaagccggta atcgccagca
      541 cttttccgcc cactcgcagc atctcatctg ccgccaggtt taactcgcgt cgtacaccgg
      601 tgtaggaaat ggctaataac agatcgtcag gggacgacgc ctgtactgta gcgagtagcg
      661 catgcatatc gcgcactgcg gcagcattga agccaatctt catcagcttc caggcaaagt
      721 tttgcgccac cagacccgaa gcgccaatac cggtcagaat tatccgccgc gcagagcgca
      781 acattgccac gcattcatgc agtttctctt cactattaac gtttaacgtt gcgtacatag
      841 cggcagtatt ttctttaatc agtttttcgc cgaccagccg taacggatca tcaccgcgga
      901 tttggttatg aatgggcacg gagggagatt ccggctggct tgccagcgct tcactcaacg
      961 ccaacttaag tgccggaaaa cctttatagc cgagtttttg cgcgaacttc acgacgctgg
     1021 actgactgac tccggcttcg ttggccagtt gctgagagct taaatggcgc gccgtatcag
     1081 gttgtagcag cagataatcc gccagttttt tatcactttg ggcaagcccc tggtaacgct
     1141 gacggatacg aagtaagccg ttcatatctt tcctcgcgat gaaatagtgg cgacaacagg
     1201 caatatgaga cacacgcgag tgaattttat attccattgc cgctaattgc catgaattaa
     1261 aaattccagt tagccatgat gcggtcgatt gttgccggaa aagtgatgat ctatctgtta
     1321 ggctatgtag tagcgaaaat taatcaggat gtttcagtcc agaggagtat ggtttggcaa
     1381 ctcacgagcg tcgtgtggtg ttttttgact tagatggaac attgcatcag caggatatgt
     1441 tcggcagttt tctgcgttat ttactgcgtc gccaaccgct gaatgcgtta cttgtcctgc
     1501 cgttgttgcc gatcatagcc attgcgttat tgataaaagg tcgtgcggca cgctggccga
     1561 tgagtctgct tctgtgggga tgcacttttg gtcacaatga agcgcgtttg caggcgttgc
     1621 aggccgattt cgtacgctgg tttcgtgaca atgttaccgc ctttccgctg gttcaggagc
     1681 gattaaccac ctacctgtta agttccgatg ctgatatctg gttgattacc ggctctccgc
     1741 agccgctggt tgaagcggtt tatttcgata cgccctggct gccgcgggtt aatcttatcg
     1801 ccagccaaat tcagcgtggc tatggtggtt gggtattgac gatgcgttgt ctgggacatg
     1861 aaaaggtcgc acaactggag cgcaaaatcg gcacgccgct gcggctgtac agtggctata
     1921 gcgacagtaa tcaggacaat ccgctgcttt atttctgtca gcatcgttgg cgagtaaccc
     1981 cgcgcggtga actccagcaa ctggaataga gtaaagcata gcgtccgtgt ataatgcgcc
     2041 gcgcttttat aaccggagtt ttctttttgt ctgaagtcga atttagccac gaatactgga
     2101 tgcgtcacgc gctgacgctg gcaaagcgtg cctgggatga gcgggaagtg ccggtcggcg
     2161 cggtattagt gcataacaat cgtgttattg gcgaaggctg gaaccgtccg attggtcgac
     2221 atgatcccac cgcacatgca gaaatcatgg ccctgcggca gggcggtctg gtgatgcaaa
     2281 attatcgact gatcgacgcc acgctgtatg tcacgcttga accctgtgta atgtgtgccg
     2341 gagcaatgat ccacagtcgc attggtcgcg tggtctttgg tgcgcgtgac gcgaaaactg
     2401 gcgctgcggg atctttaatg gatgtgctgc atcatccggg tatgaatcac cgggtggaaa
     2461 ttaccgaagg aatactggcg gatgagtgcg cggcgttgct cagtgacttc tttcgcatgc
     2521 gccgccagga aattaaagcg cagaaaaaag cgcaatcctc gacagattaa ttttgtttct
     2581 cttcactccc ttttctggaa aacagcagag agggagagtg ggtcaaatag tttgatgacg
     2641 actgggaaag aaacgagaga aaaggaaatt tctctttgcc cagctccgta ggcgataccg
     2701 ccggataatc ctgcgccagt tgcatcgccg cttctgtagc ctgcttctct ttctcctgca
     2761 gataacccac caggctaatc tgatacttac gaatattttc gacataagcg taggcttcat
     2821 gcccacgagc gtagccgtaa gtcagcttgc tgtaataggg tttctggcta agtaaaggca
     2881 gacgctgttt tacgtcagcc caactgtcag gattaccttt ggtttttgcc gtcagggcgc
     2941 gggcatccag catatgcgca tagcccatat tgtacgcggc aagggcaaac cagatccgtt
     3001 cgttctccgg cacactttcc ggcactttac tcatcatatc ctgcaaataa cgcacgccgc
     3061 cgctgatgct ctgttcggca tcggtacgat ccgtaatgcc gaggctttgc gcggtgtttt
     3121 tggttaacat catcatgccg cgcacacccg tcggtgaagt ggcctgagca tcccagtgcg
     3181 attcttgata agcaatagcg gccagcaaac gccagtcaat ttcttcggcg tatttctcaa
     3241 acaggggctt taactgcggc agtaccgcat cgacggcgcg taaaaatgtg cgcgtatcga
     3301 cgtaatcaaa atcatcgcca tgccccaggt atttctcttc aatgcgtgcc agcgtaccgt
     3361 cttcattcat ctcattgaag aaatcgagca gagcggcgga aagggtatta tcgccatcta
     3421 acgggctaaa ccaggtcacc ggttgttcat cggtgatatc gagcgctacg gcgagttccg
     3481 ggtgaacgcg ctgaaacagg ctgatggcga cagaatcagc aatggtgtaa tcgagttttc
     3541 cttcgatgac atcttccatt aattccgcag agcctttttt gtcgtctacc ttccagctta
     3601 attccgggaa ttttgtttct ttcagggtct ggagatcgtt aaccaccaca tgacccggtg
     3661 caacggtgag ttgctccgcc gtcaggttgc ccagtgtacg tgggcgatac tgacccactt
     3721 tataaaccag ttgttgtgac acggaataat aggtagggcc aggctgataa ttttttacac
     3781 gctcactgtt atagaccagg cctgccgcca gcaggtcggc gttaccatta tcgaggtcgt
     3841 caaacagctg gctgatattc tgccgcacgg tcactttcag ttttacgccg aggtaatcgg
     3901 caaactgttt cgccagttcg taatccaggc caaaaggttt cccgttgatt tcgttataag
     3961 tcaggggggt atgaatggtg ctcacacgca actctccccg cgcttgaatg gcggcgatac
     4021 ggttgtcggc tttaccaaac cagggaatgg atggccagag agcgaccgcg agcagcagtg
     4081 ccagaatgcc gatgaacaga taattaatct ttaatttttt caattagtta attctctgtg
     4141 tcgtgcgcgt cccagcttga aaaaacgtaa taatagtgaa aggtttactc ataaatgagc
     4201 ggcattttgc gtaaacctgc gccagttggc aacttattac agccattggc ggcacgcgtt
     4261 gctaattcac gatggtgatt ttatttccac gcaaacggtt tcgtcagcgc atcagattct
     4321 ttataatgac gcccgtttcc cccccttggg tacaccgaaa gcttagaaga cgagagactt
     4381 atgatggaaa ttctgcgtgg ttcgcctgca ttgtcggcat tccgaatcaa caaactgctg
     4441 gcacgttttc aggctgccag gctcccggtt cacactattt acgccgagta tgtccatttt
     4501 gctgacctca atgcgccgtt aaacgatgat gagcacgcac aacttgaacg cctgctgaaa
     4561 tatggcccgg cactcgccag ccacgccccg caaggcaaac tcctgctggt gaccccgcgt
     4621 cctggcacca tctctccctg gtcttcgaaa gcgaccgata tcgcccataa ttgcggtcta
     4681 caacaggtaa accgccttga gcgcggcgtt gcttactatg tagaagccgg tacgctgacc
     4741 aatgaacaat ggcagcaggt taccgctgaa ctgcacgacc gcatgatgga aacggtcttt
     4801 tttgctttag atgatgcaga gcagctgttt gcacaccatc aaccgactcc ggtcaccagc
     4861 gttgatttgc tggggcaggg ccgtcaggcg ctaatcgacg ctaacctgcg tcttggcctg
     4921 gcactggcgg aagatgaaat tgactatttg caggatgctt tcacaaagct gggtcgtaac
     4981 ccgaacgaca tcgaactgta tatgtttgcc caggcgaact ccgagcactg ccgccacaaa
     5041 atttttaacg ccgactgggt tatcgatggt gaacagcagc cgaaatcact gtttaagatg
     5101 atcaaaaaca ccttcgaaac cacgccggat cacgttctct ctgcttataa agataacgcc
     5161 gccgtaatgg aaggttctga agtgggccgc tactttgctg accacgaaac gggccgctac
     5221 gatttccatc aggaacctgc gcatatcctg atgaaagttg aaactcacaa ccacccgacg
     5281 gcgatttctc cgtggccggg cgcggcgacc ggttccggcg gtgaaatccg cgatgaaggt
     5341 gcctccgggc gcggcgcgaa gccgaaagct ggtctggtcg gtttctccgt gtccaacctg
     5401 cgaattcctg gcttcgaaca gccgtgggaa gaagatttcg gtaaacctga gcgcattgtt
     5461 accgcgctgg acatcatgac cgaaggcccg ctgggcggtg cggcgtttaa caacgaattt
     5521 ggtcgtccgg cactgaatgg ctacttccgt acttatgaag aaaaagtgaa cagccacaat
     5581 ggcgaagagc tgcgcggtta tcacaaaccg atcatgctgg cgggcgggat cggcaacatt
     5641 cgtgccgatc acgtacaaaa aggcgagatc aacgttggtg cgaagctggt cgttctcggc
     5701 ggcccggcaa tgaacatcgg tcttggcggc ggcgcggcgt cttccatggc gtctggtcag
     5761 tctgatgccg acctcgactt cgcttccgta cagcgcgaca acccggagat ggagcgtcgc
     5821 tgccaggaag tgatcgaccg ttgctggcag cttggtgatg ccaacccaat cctgtttatc
     5881 cacgacgttg gcgcaggcgg tctttctaac gccatgccgg aactggtgag cgacggcggg
     5941 cgcggcggta aattcgaact gcgcgatatc ctgagcgacg aaccgggcat gagcccgctg
     6001 gaaatctggt gtaacgaatc ccaggaacgc tacgtgctgg cagttgctgc cgatcagtta
     6061 ccgctgtttg acgaactgtg taagcgtgag cgcgcgccat acgcggtgat tggtgaagcg
     6121 accgaagaac tgcatctttc tttgcacgat cgtcattttg ataatcagcc gatcgatctg
     6181 ccgctggacg tactgcttgg taaaacgccg aagatgaccc gcgaagtaca aacgctgaaa
     6241 gcgaagggtg atgcgctggt tcgtgaaggg attaccattg ctgacgcggt gaaacgcgtg
     6301 ctgcacctgc cgaccgtggc ggagaaaact ttcctggtga ccattggtga ccgcagcgta
     6361 accggcatgg tggcgcgcga tcagatggtg ggtccgtggc aggtgccggt cgctaactgc
     6421 gcggtcacta ccgccagcct cgatagctac tacggtgaag cgatggcgat tggcgagcgt
     6481 gcgccggttg cgctgctgga tttcgccgct tctgcccgtc tggcggtcgg tgaagcgtta
     6541 accaacatcg ccgcaacaca aattggcgat atcaaacgca ttaaactttc cgccaactgg
     6601 atggcggcgg caggccaccc tggtgaagat gcaggcctgt atgaagccgt taaagccgtg
     6661 ggtgaagaac tttgtccggc gctgggcctg acgatcccgg tgggtaaaga ctctatgtcg
     6721 atgaaaaccc gttggcagga aggtaacgaa gagcgcgaaa tgacgtcgcc gctgtcgctg
     6781 gtgatttctg catttgcccg cgtggaagat gtacgtcaca ccatcacgcc acagctttct
     6841 accgaagata acgcactgct gctgattgat ttgggcaaag gcaataacgc gctgggcgca
     6901 acggcgctgg cgcaggttta tcgtcagctt ggcgacaaac cggcagatgt acgcgatgtc
     6961 gcgcaactga aaggcttcta tgacgcgatt caggcgctgg ttgcacagcg taagctgctg
     7021 gcgtatcacg accgctctga tggcggcctg ctggtaacgc tggcggaaat ggcctttgct
     7081 ggtcattgcg gcattgacgc ggatatcgcc tctctgggtg acgatcgcct ggcggcgttg
     7141 tttaacgaag aactgggtgc ggtgattcag gttcgtgccg ctgaccgtga agccgttgaa
     7201 gcggtactgg cacagcatgg gcttgcggat tgcgtccatt atgtagggca ggcggtttcc
     7261 ggtgaccgtt ttgtgattac cgccaacggg cagactgtat tcagcgaaag ccgcactacg
     7321 ttgcgtgtct ggtgggcaga aactacctgg cagatgcagc gcctgcgtga caacccggag
     7381 tgtgccgatc aggagcacca ggcgaaatca aacgacgccg atccgggcct gaatgtgaaa
     7441 ctgtcgttcg atatcaacga agatgtggca gcaccgtata ttgccactgg tgcgcgtccg
     7501 aaagttgccg tgctgcgtga gcagggggtg aactcgcatg ttgaaatggc ggcagctttc
     7561 caccgtgcag gcttcgatgc aatcgatgtg catatgagcg acctgctggc tggacgcacg
     7621 ggcctggagg atttccacgc tctggtagcg tgcggtggat tctcctacgg tgacgtgctg
     7681 ggtgctggtg aaggctgggc gaagtctatc ctgttcaatg accgtgtacg cgatgagttt
     7741 gcaaccttct tccaccgtcc gcaaacgctg gcgctggggg tatgtaacgg ttgccagatg
     7801 atgtctaacc tacgcgaact gatcccaggt agtgagttgt ggccacgttt tgtgcgcaat
     7861 acctccgatc gctttgaagc gcgtttcagc ctggttgaag taacccaaag cccgtctctg
     7921 ctgttgcagg ggatggtggg ctcgcaaatg ccgattgctg tctctcatgg cgaagggcgc
     7981 gtggaagtgc gtgatgcggc gcatctggcg gcactggaaa gcaaagggct ggtggcactg
     8041 cgctatgtcg ataacttcgg caaagtcact gaaacctacc cggctaaccc gaacggttct
     8101 ccgaatggta ttacggcagt cacgactgaa agtggtcgag tcaccattat gatgccgcac
     8161 ccggaacgtg ttttccgtac tgtcagcaac tcctggcatc cggaaaactg gggcgaggat
     8221 gggccgtgga tgcgtatttt ccgcaatgca cgtaagcagt tggggtaagc cgtcaaccca
     8281 ttggttttgc agccggatga ggcattaagc accaactact attataagct cccaacttcg
     8341 ggagcttttt tgtgtctgta aatcacgaca atgggtggtt taccgcgtcg ctttctggcg
     8401 acacttaact aattgatttt aatggcatct aataagttta tcttaagttg tctcttttta
     8461 gcgacacagt ggctgattta cagccaaata gcgaagaagc cataacccct tcaaaacagc
     8521 gtacaatcag tacgttacca aactattttc tttattggca cagttactgc ataatagtaa
     8581 ccagtggctc attcaccgac ttatgtcagc cccttcggga cgtgctacat aaaatacgaa
     8641 tgacgcacaa caaggtgcct gccgtccaac ttctgatatc agcgtagcta tatcaaccat
     8701 cgggcgaaac gtcgagttag gcaccgcctt attccataac aaagccgggt aattcccggc
     8761 tttgttgtat ctgaacttcc cctcggttag catcaggcta ttcgcgtctg acgagagtaa
     8821 caccttgaaa cgctggcccg tttttccccg ctcattacga caactggtaa tgctggcatt
     8881 tttgctgatt ctgctgcccc tgttggtgct ggcatggcaa gcctggcaaa gcctgaatgc
     8941 gcttagcgat caggcggcgc tggttaaccg cactacgctt attgatgccc ggcgtagtga
     9001 agcgatgacc aacgcggcgc tggagatgga gcgtagctac cgtcagtatt gcgtgctgga
     9061 cgaccctacg cttgcgaagg tttatcaaag ccagcgcaag cgttacagcg aaatgctcga
     9121 tgcccacgca ggcgtactgc cagacgataa actttaccag gcattgcgtc aggatttgaa
     9181 caatctggcc caacttcagt gtaacaacag tggtcccgat gctgccgccg ccgcgcgtct
     9241 tgaagccttt gccagtgcca ataccgaaat ggtgcaggcc acgcgcacag tggtgttctc
     9301 tcgtgggcag caacttcagc gtgaaatcgc cgaacgtggg caatattttg gttggcaatc
     9361 gctggtgcta tttctggtga gtctggtgat ggtgctactt ttcacgcgga tgattatcgg
     9421 gccggtgaaa aatatcgagc gcatgatcaa ccgtctgggg gaagggcgtt ctctgggcaa
     9481 tagcgtttcg ttcagtggac cgagcgagtt acgctcggtt gggcaacgta ttctttggtt
     9541 aagtgagcgc ctgtcatggc tggaatccca acgccatcaa tttttaagac atttatctca
     9601 tgaattaaaa acgccactgg cgagtatgcg cgagggcact gaattactgg ctgaccaggt
     9661 tgtcgggccg cttacgccag agcaaaaaga ggtggtgagc attcttgata gcagcagccg
     9721 caatttgcaa aaactgatcg aacaactgct tgattacaac cgtaaacagg cggacagtgc
     9781 ggtggaactg gagaatgttg aattagcacc gctggtggag acagtggttt ctgctcatag
     9841 cctgcccgca cgggctaaaa tgatgcatac cgacgtcgat ctcaaagcaa cagcttgcct
     9901 ggcggagcca atgctgctga tgagcgtact ggataatctt tactccaatg cggtgcacta
     9961 cggggctgaa tccggtaaca tttgccttcg cagcagttca catggtgcgc gggtttatat
    10021 tgatgtcatc aatacaggca cgcccattcc gcaagaggaa cgcgccatga tcttcgaacc
    10081 cttttttcag ggaagccacc agcgaaaagg ggcggtgaag ggcagcggtc tgggattaag
    10141 cattgccagg gattgtattc gccgtatgca aggggaactg tatctggtcg acgagagcgg
    10201 gcaagacgtt tgtttccgca ttgatttacc gtcgtcgaaa aacacgaaat aatgctaaca
    10261 gcccatccca tcaggctatt ttatttgtca gttcggcccc gggcagtgct cgaaatcttc
    10321 acgtacgaca tgtacgctct ggtttctccg cgctgtccat gtccaaactg cctgcaacaa
    10381 ttacgcccac tgggataggc tttaagtctg gtgaatatgc gacacatttt tcaacgatta
    10441 ttgccccgac ggctgtggct ggcagggctg ccatgtctgg cattgctggg ttgcgtgcag
    10501 agtcacaata agccagccat tgatacgcca gcagaagaaa aaattccggt ttatcaactg
    10561 gctgactatc tttctactga atgtagcgac atctgggcgc tgcaaggtaa atcaaccgaa
    10621 accaatccgc tttactggct gcgggcgatg gattgtgctg atcgtttaat gcctgcgcag
    10681 tcgcgtcagc aagcccgcca atatgacgac ggcaactggc aaaatacctt taaacaaggg
    10741 attttgctcg ccgacgccaa aattacgccg tatgagcgcc gccagctggt cgcgcgcatt
    10801 gatgcgctaa gtaccgagat cccggcacag gttcgccctc tgtatcaact ctggcgcgac
    10861 ggtcaggcgc tgcaactgca actggcggaa gagcgccaac gctacagtaa actccagcag
    10921 tccagcgaca gcgagctgga tactttgcgc cagcaacatc acgttctaca acagcaactg
    10981 gaactcacta cccgcaagct ggaaaacctg accgatattg aacgccagct ctcgacccgc
    11041 aaaccggctg gaaatttctc gccggatacg ccgcacgaga gtgaaaagcc cgcgccatcc
    11101 actgatgagg tcactcctga tgagccataa acctgcgcat ttattactgg tcgatgacga
    11161 tccgggattg ctgaaactgc ttggcctgcg cctgaccagc gaaggctaca gcgtggtcac
    11221 ggcggaaagt ggtgctgaag gattacgggt actgaatcgc gaaaaagtag atttagttat
    11281 cagcgacctg cggatggatg aaatggatgg tatgcagctg tttgctgaaa tccagaaagt
    11341 gcagccggga atgccggtaa ttattcttac cgcacatggt tctattcccg atgccgttgc
    11401 tgcaacacag cagggagtgt tcagtttcct caccaagcct gtcgataaag acgcgctata
    11461 tcaggcaatt gacgatgcgc tggaacaatc cgcgccagcc accgatgaac gctggcgcga
    11521 ggcgattgtc acccgcagcc cgctgatgct gcgtttgctg gaacaggcgc ggctggtggc
    11581 gcaatcagac gttagcgttt tgattaacgg tcagagcggc accgggaaag agattttcgc
    11641 ccaggctatc cacaacgcca gcccgcgcaa tagcaaacca tttattgcta ttaactgtgg
    11701 tgcattgccc gagcaattgc tggagtcgga gctgtttggt catgcgcgtg gcgcgtttac
    11761 tggcgctgtc agcaatcgcg aaggtttatt ccaggcggcg gaaggcggta cgctatttct
    11821 cgatgagatt ggcgatatgc ctgcaccgtt gcaggtcaaa ctgctgcgtg tgttgcagga
    11881 gcgtaaagtg cgcccgctgg gcagtaaccg cgatattgat atcgacgtgc ggattatttc
    11941 cgccactcac cgcgacctgc caaaagcgat ggcgcgcggg gaattccgtg aagatctcta
    12001 ttaccgcctc aacgttgtca gcctgaaaat tccggctctg gcagagcgca cagaagacat
    12061 tccgctactg gcaaatcacc tgttgcgcca ggcggcagag cgacataaac cgtttgtccg
    12121 cgcgttctcc accgatgcga tgaaacgcct gatgaccgct agctggccgg gtaatgtacg
    12181 ccagttggtc aacgtgattg aacagtgcgt ggcgctgacc tcatctccgg tgattagcga
    12241 tgcattagta gagcaggcgc tggagggcga aaacacggcg ctgccaacct ttgttgaggc
    12301 acgtaatcag tttgaactca actatttgcg taagctgctg caaatcacca aaggcaacgt
    12361 cacccacgcg gcgagaatgg cggggcgcaa ccggacagaa ttttataaac tgctttcccg
    12421 acacgagctg gatgcaaacg atttcaagga atgaattggc gttatgtgtt acgtttagca
    12481 gatcaaaaga caggcgacct tttcaaggaa tagcatgaaa aagattgatg cgattattaa
    12541 acccttcaag ctggacgatg ttcgcgaagc actggctgaa gtcggcatta ccggcatgac
    12601 ggtcactgag gtgaaaggtt ttggccgcca gaaaggccat accgagctgt accgcggcgc
    12661 ggagtatatg gtggattttc tgccgaaagt gaaaattgag attgtcgtac cggacgacat
    12721 tgtcgatacc tgtgtcgata ccattattcg cacggcgcaa accggcaaaa tcggtgacgg
    12781 taaaatcttc gtctttgacg tggcacgggt cattcgtatc cgtaccggtg aagaagacga
    12841 cgcggcaatt taaaccgcgt caagggttgc cggatgtttc catccggcaa catcaaatca
    12901 cagcacctta tgcgggccaa agcattcgta atgaatattt tcctgcttca cgcccagatc
    12961 cactaactgt ttcgcggcaa actgcatgaa gccaaccggg ccgcagagat agaactgcat
    13021 tgtcggatcg ctgaacgcac cttccagttt gctcaaatcc atcagacctt cgctatcaaa
    13081 ctgaccttta gcgcgatcgg cttcgctcgg ctgacgatac caggtgtgcg cggtaaagcg
    13141 cggcagtgac agccccagtt ccttaacttc atcagcaaag gcgtgaacat cgccattttc
    13201 tgccgcatgg aaccagttca cttgtgctgt gtggcctgct tttgccagtg tgtcgagcat
    13261 tgccagcatt ggcgtttgac cgacaccggc agagattaac gtcactggtg tgtcatctgc
    13321 gacagccata aaaaaatcac ctgctggagc gaccagtttc acgacatcgc caacattggc
    13381 gtgattgtgc agccagttgg atacctgccc accctcttcg cgtttcaccg caatacgata
    13441 gcctttgcca tccggtttgc gagtcaaaga gtactgacga atttcctgat gcgggaaacc
    13501 ttccggcttc agccagacgc cgagatattg ccccggacgg tattctgcca ctgcgccacc
    13561 gtcgaccggc tccagttcga agctggtgat aagcgcgctg cgcggtgttt tagccacaat
    13621 gcggaaatcg cgagtacctt cccagccacc ggctttgctg gcgttttcgt tatagatttc
    13681 cgcctcgcga ttgataaata cattagccag tacgccatag gctttacccc acgcgtccag
    13741 cacttcctgg cccgggctga acatttcgtc cagcgtcgcc aacaggtgtt caccgacaat
    13801 gttgtactgt tccggtttga tctggaagct ggtgtgcttc tgcgcgattt tttctaccgc
    13861 tggcagcagc gcaggcaggt tttcaatatt actggcgtag gcggcaatag cgttaaacag
    13921 ggcttcacgt tgatcgccat tgcgctggtt actcatgtta aaaatttctt tgagttctgg
    13981 gttatgagta aacatacggt cgtagaaatg ggcggttaac tttggtcccg tttccaccag
    14041 taaagggatg gtggctttta ctgtagcgat ggtttgagcg tcaagcatat ggtcttcctt
    14101 ttttttgcat cttaattgat gtatctcaaa tgcatcttat aaaaaatagc cctgcaatgt
    14161 aaatggttct ttggtgtttt tcagaaagaa tgtgatgaag tgaaaaattt gcatcacaaa
    14221 cctgaaaaga aatccgtttc cggttgcaag ctctttattc tccaaagcct tgcgtagcct
    14281 gaaggtaatc gtttgcgtaa attcctttgt caagacctgt tatcacacaa tgattcggtt
    14341 atactgttcg ccgttgtcca acaggaccgc ctataaaggc caaaaatttt attgttagct
    14401 gagtcaggag atgcggatgt taaagcgtga aatgaacatt gccgattatg atgccgaact
    14461 gtggcaggct atggagcagg aaaaagtacg tcaggaagag cacatcgaac tgatcgcctc
    14521 cgaaaactac accagcccgc gcgtaatgca ggcgcagggt tctcagctga ccaacaaata
    14581 tgctgaaggt tatccgggca aacgctacta cggcggttgc gagtatgttg atatcgttga
    14641 acaactggcg atcgatcgtg cgaaagaact gttcggtgct gactacgcta acgtccagcc
    14701 gcactccggc tcccaggcta actttgcggt gtacaccgcg ctgctggaac caggtgatac
    14761 cgttctgggt atgaacctgg cgcatggcgg tcacctgact cacggttctc cggttaactt
    14821 ctccggtaaa ctgtacaaca tcgttcctta cggtatcgat gctagcggtc atatcgacta
    14881 cgccgatctg gaaaaacaag ccaaagaaca caaaccgaaa atgattatcg gtggcttctc
    14941 tgcatattcc ggcgtggtgg actgggcgaa aatgcgtgaa atcgctgaca gcatcggtgc
    15001 ttacctgttc gttgatatgg cgcacgttgc gggcctggtt gcagctggcg tctacccgaa
    15061 cccggttcct catgctcacg ttgttactac caccactcac aaaaccctgg cgggtccgcg
    15121 cggcggcctg atcctggcga aaggtggtag cgaagagctg tacaaaaaac tgaactctgc
    15181 ggttttccct ggtggtcagg gtggtccgtt gatgcacgta atcgccggta aagcggttgc
    15241 tctgaaagaa gcgatggagc ctgagttcaa aacttaccag cagcaggtcg ctaaaaacgc
    15301 taaagcgatg gtagaagtgt tcctggaacg cggctacaaa gtggtttccg gcggcactga
    15361 taaccatctg ttcctggttg atctggttga taaaaacctg accggtaaag aagctgacgc
    15421 cgctctgggc cgtgctaaca tcaccgtcaa caaaaacagc gtaccgaacg atccgaagag
    15481 cccgtttgtg acctccggta ttcgcgtggg tactccggcg attacgcgtc gcggcttcaa
    15541 agaagcagaa gcgaaagaac tggctggctg gatgtgtgac gtgctggaca gcatcaatga
    15601 tgaagccgtt atcgagcgca tcaaaggtaa agttctcgac atctgtgcac gttacccggt
    15661 ttacgcataa gcgaaacggt taatctgttg aaaaaggctt ccttcgggaa gcctttctac
    15721 gttatcgcgc catcaaatct gtcgtaactg cgcctcaaca tacaaatagc caatccccag
    15781 cacctgttgt gcgcggctta attgcccaaa gccaattttc gtcgctttca cgctcggtcc
    15841 ttcgtcgcgg tcgaacgggt taaatcccgt ctggcgaata atggtattaa gccagtgctc
    15901 accaaaggca caactgcgac cgtacaacca aatctgatta atattgagga tgttgaggaa
    15961 gttatacaga ctcaaaccaa tggcattggc agagcgatca acccagctgg tgatccacgg
    16021 ttctccactc tgccaggcag cgattaactg cgctgttgtc agtttttcag gatcaagctg
    16081 agtattaacc ggttgtgatt ttagccatac ccgcgcctgt ttttttaatg cacttaacga
    16141 ggcgacggtt tccaggcagc cgtaacgtcc acagtcgcag acgacgccat ccggattaac
    16201 gatggtgtga ccaatctgtc cgctaccata caaacagccg cgataaattt gctcgttaat
    16261 cacgaacgac gagccaatgc cgtaatcaac gttgatcacg cagaaatccc gttcctgcga
    16321 attattttgc catttctccg ccagcgccag catcacgcag tcattatcga ccatcacccg
    16381 aatgccgagc ttctcttcca gcagatactt tacctcaacc ggcgttgtcc acggcgcttg
    16441 cggcatggtt tgcgacacgc cggtcaccgg atcaacctga ccgtggattg ccagcgccag
    16501 gttgatggta cggtccggcc acaatttacg gtggcgatgc cagcattttt cgatttcgga
    16561 cagcagcgcc tgcggcgtcg gtgcatcaat ctgtaaatac tcaaattcac ctttcggact
    16621 taaacaagca ttagcaacct gacactcaat actggtgggc gtcacgttca ggcacaacgt
    16681 ccagtcacct tccggcgcaa tcagccatgt accgctacta tgcccgcgcg tctggctttc
    16741 atcgtcgata ttcaccaccc gtttttcgct ttccagttcc tgcaaaatat tactgaccgc
    16801 cggaatcgaa atttgcgcca gccgggccag cgttgattta ttggcgcgct tttgccggta
    16861 cagcagttcc agaatcacgc atttgttatg gtggcgaatc tgttgattat taatgcaggc
    16921 tctcatcttc actaaacctt gtttagttaa ttgcgtgatt attccgcgaa aacattgcca
    16981 gattgttcaa tacactgcca caaatctttt aattcagtat gtcttgttaa tattgagggc
    17041 accatgactc cagtaaaagt gtggcaagag cgcgttgaga tcccgaccta tgaaaccggg
    17101 ccgcaggata tacatcccat gttcctggaa aatcgcgttt atcagggatc gtccggcgcg
    17161 gtttatccct acggcgtgac cgatacgctg agcgagcaga aaaccctgaa atcctggcag
    17221 gcggtgtggc tggaaaacga ctacatcaaa gtgatgatcc tgccggagct gggcggtcgg
    17281 gtgcatcgcg cgtgggataa agtgaaacag cgcgattttg tctatcataa tgaagtcatt
    17341 aaaccggcgc tggtggggtt gctggggccg tggatctccg gcgggattga gtttaactgg
    17401 ccgcaacacc atcgcccgac cacctttatg cccgtcgatt tcaccctcga agcccatgaa
    17461 gacggcgcac aaaccgtgtg ggtcggcgaa acggagccga tgcacggttt acaggtgatg
    17521 acaggtttca ccctgcgccc tgaccgggcg gcactggaaa ttgccagccg cgtctataac
    17581 ggcaacgcca cgccgcgtca tttcttgtgg tgggccaacc cggcagtgaa agggggtgaa
    17641 gggcatcaga gcgtcttccc gccggatgta acggcggtgt ttgatcacgg caaacgggcc
    17701 gtctccgctt tccccatcgc caccggcact tactacaaag tggactactc cgccggagtg
    17761 gacatttctc gctataaaaa tgtgcccgtt ccaacctcat atatggctga aaaatcacag
    17821 tacgattttg ttggcgcgtg gtgtcacgat gaagatggcg gtttgctgca cgttgccaac
    17881 caccatattg cgccaggtaa aaaacagtgg agctggggac acagtgaatt tggccaggcg
    17941 tgggataaga gtctgaccga caataacggc ccgtatatcg aactgatgac tggtattttt
    18001 gccgataacc agcccgattt tacctggctt gatgcttacg aagagaagcg ttttgagcag
    18061 tatttcctgc cttaccattc tttgggcatg gtgcaaaacg cctcccgcga tgcggtgatt
    18121 aaactccagc gtagcgagcg ggggattgag tgggggctgt atgccatctc tccgttgaaa
    18181 ggataccgtc tggcgatccg cgaaatcggc aaatgcaacg cgttgctcga tgatgccgtg
    18241 gccctgacac cagcgaccgc catccagggc gtgttgcacg gtatcaatcc tgaaaggctg
    18301 accattgaac tctccgatgc cgacggcaat atcacactga gttatcagga acatcagccg
    18361 caagagttgc cgttgccgga cgtcgccaaa gcgccactgg cagcacaaga cattaccagt
    18421 acagatgaag cctggtttat cggtcagcat ctggagcaat atcatcacgc cagccgttca
    18481 ccgttcgatt actacctgcg cggcgtggcg ctggatccgc tggattatcg ctgtaacctg
    18541 gcgctggcga tgctggaata taaccgagca gatttcccgc aagcggtggc gtatgccact
    18601 caggcgctga aacgcgcaca tgcgctaaac aaaaatccgc agtgcggaca ggcgagtttg
    18661 attcgcgcca gtgcttacga acgtcagggg caatatcaac aagccgaaga ggatttctgg
    18721 cgggcggtct ggagcggcaa cagtaaagca ggtggctatt atggtctggc acggctggcg
    18781 gcgcgtaatg gtcacttcga cgctggtctg gatttttgcc aacaaagtct tcgcgcctgc
    18841 ccaaccaatc aggaagtgct ttgcctgcat aatctgctgc tggtgttaag tggtcgtcag
    18901 gacaacgcgc gtttgcagcg cgagaaactg ctgcgtgatt atccgctgaa cgccactctg
    18961 tggtggctga actggttcga tggtcgtagc gaatcagccc tcgcgcagtg gcgcggtctg
    19021 tgtcagggac gcgacgttaa cgccctgatg accgccgggc aactgattaa ctggggaatg
    19081 cccaccctgg cggcagagat gctgaacgca ctggactgcc agcgcacgct gccgctttac
    19141 ttgcaagcca gcttgctgcc gaaagctgaa cgcggcgaac tggtcacaaa agccattgat
    19201 gtcttcccac agtttgtccg tttcccgaat acgctggaag aagtggcggc gctggagagt
    19261 attgaagagt gctggtttgc ccgtcattta ctggcctgct tctactacaa caagtgcagc
    19321 tacaacaaag ccattacttt atggcaacgt tgcgtagaga tgtcgccgga gtttgccgac
    19381 ggctggcgcg ggttagcgat ccatgcgtgg aataagcaac acgattatga gctggccgcg
    19441 cgttatcttg ataatgctta tcagcttgcg ccgcaggatg cacgtctgct tttcgaacgg
    19501 gatttgctgg ataagttaag cggtgtcaca ccggagaaac gactggcgcg tctggaaaat
    19561 aatctggaaa ttgcgctgaa acgcgacgac atgaccgcag aactgctcaa tttgtggcat
    19621 ctcacgggtc aggcagacaa agcggcggac attctcgcca cgcgcaaatt ccacccgtgg
    19681 gaaggcgggg aagggaagat caccagtcag tttatcctca accagttatt acgcgcctgg
    19741 cagcatcttg atgccagaga gccgcagcag gccagcgaac tgcttcatgc cgcgttgcat
    19801 tatccggaga atttaagcga aggccgttta ccggggcaaa ctgacaacga catctggttc
    19861 tggcaggcga tatgcgccaa cgcgcagggc gatgaaactg aagcgacgcg ttgtttacgt
    19921 ctggcggcga ctggcgatcg caccattaac attcacagtt attacaacga tcagccggtt
    19981 gattatctct tctggcaagg aatggcgctg cgactgctgg gcgaacaaca caccgcacag
    20041 caactgttta gtgaaatgaa acagtgggcg caagagatgg cgaaaaccag tatcgaggcg
    20101 gatttctttg ctgtttcaca acctgacctg ttgtcgctgt atggcgattt acaacagcag
    20161 cataaagaaa aatgcctgat ggtggcgatg ctggcgtccg cgggactcgg ggaggttgcg
    20221 cactatgaat ctgctcgcgc tgaattgatg gcgattaatc cagcctggcc gaaagcggca
    20281 ttattcacca ccgtgatgcc ttttattttt agctacgttc actaaacctt attaactgta
    20341 atatttgagc ggcacgcgct gccgctcacg ctcaaataat taaaataagc cgttgtaaat
    20401 aaaaacccta caggaggcac tatgcctaaa aaaatgacaa gaacccgtaa tttattatta
    20461 atggctacgc tattgggtag cgcactattt gccagggctg cggataaaga aatgactatt
    20521 ggcgcaatat accttgatac ccagggatat tacgccggag tgcgtcaggg cgttcaggat
    20581 gcggcaaaag attcttcagt acaggtacag ttaattgaaa ctaacgctca gggcgatatt
    20641 tcgaaagaaa gtacctttgt tgataccctc gtggcgcgta atgtcgatgc cattatttta
    20701 tcggcagtgt ctgaaaatgg cagtagtcgt accgttcgtc gcgccagtga agcgggtatt
    20761 ccggtgattt gctacaacac ctgtattaat caaaaaggtg tcgataaata tgtctcggcg
    20821 tatctggtcg gcgatccgct ggaatttggt aaaaaactgg gtaacgctgc cgccgattat
    20881 tttattgcca ataaaattga ccagccgaaa attgccgtca tcaattgcga agcctttgaa
    20941 gtttgtgtgc agcgacgtaa aggatttgaa gaggtattaa aatcccgcgt tcccggcgcg
    21001 caaatagtcg ctaatcagga agggactgtt ttagataaag cgatttccgt tggtgaaaaa
    21061 ctaattatct ccacgccgga tctcaacgcc attatggggg aatcgggcgg tgcgacactc
    21121 ggcgcggtaa aagcggtacg taatcaaaat caggccggaa aaattgctgt tttcggttcg
    21181 gatatgacca ctgaaattgc tcaggagctg gaaaacaatc aggtgctgaa agcggtggtg
    21241 gatatttccg gtaagaaaat gggcaatgct gttttcgcgc aaactttgaa ggttatcaat
    21301 aaacaagccg acggtgaaaa agtgattcag gtgccaatcg atctctatac caaaacggaa
    21361 gacggtaaac agtggctggc aacgcacgtt gatggtctgc cctaatcgct gattcgcaga
    21421 ggtccctatg ttcacagcaa cagaggcagt cccggtagca aaagtggtgg caggaaataa
    21481 gcgttatccc ggcgtcgttg cgctggataa cgttaacttc acgctcaata aaggcgaagt
    21541 tcgtgcgctg ttaggcaaaa acggtgcggg aaaatcgacc ctcattcgaa tgcttaccgg
    21601 tagcgaacgt ccggatagcg gtgatatctg gattggcgag acgcgactgg aaggtgacga
    21661 agctacgctg actcgccgtg ccgctgaact gggggtacgc gcggtttatc aggagttaag
    21721 tctggtggaa gggctgacag tggcggaaaa cctctgcctc ggtcagtggc cccgccgcaa
    21781 cggcatgatt gattacctgc aaatggcgca ggacgcccaa cgttgcttgc aggcgttggg
    21841 cgttgacgtt agtcctgaac aacttgtttc aacactaagc ccggcgcaaa agcagctggt
    21901 ggaaattgcg cgggtgatga agggcgagcc gcgcgtggtc attcttgatg aacccaccag
    21961 ctcgcttgcc agtgcggagg ttgaactggt gatcagcgcg gtgaaaaaga tgtcggcact
    22021 gggcgtggcg gtgatttatg tcagccaccg gatggaagaa attcgccgca ttgcctcctg
    22081 tgccaccgtt atgcgcgatg gtcaggtggc gggcgatgtg atgctcgaaa acacctccac
    22141 gcatcatatt gtgtcgctaa tgctcgggcg cgatcacgtt gatattgcgc cggtttcccc
    22201 tcaggaaatt gtcgatcagg ccgtgctgga agtccgtgcg ttacgccata agcccaagct
    22261 ggaggatatc agctttacgc tccgtcgtgg cgaagtactc ggcattgctg gcctgctggg
    22321 ggcagggcgc agtgaattgt tgaaagccat agttgggctg gagacgtatg aacagggcga
    22381 aattgttatc aacggcgaga aaatcactcg ccccgattac ggcgacatgc tgaaacgcgg
    22441 cattggctat acgccagaaa accgcaaaga tgcggggatc attccctggc tgggcgttga
    22501 cgaaaataca gtgctgacca atcggcaaaa aatcagcgcc aacggtgtgc tgcaatggtc
    22561 caccatccgc cgcctgaccg aagaggtgat gcagcggatg acggtcaagg ccgccagtag
    22621 cgaaacgccc atcggcacgc tttctggcgg caatcagcaa aaagtggtga tcggtcgttg
    22681 ggtctatgcc gccagccaga ttttgttgct cgacgagcca acgcgtggcg tcgatatcga
    22741 agccaaacag cagatttacc gtattgtccg cgagctggct gccgaaggaa aaagcgtggt
    22801 gtttatctcc agtgaagtgg aggagttgcc gctggtgtgc gaccgcattc tgttgttaca
    22861 gcacggtacg ttctcgcagg agtttcacgc tccggtcaat gtggatgagc tgatgtccgc
    22921 cattctgtct gtgcactgat gaattttacc gaggaacctg aagatgtctg cttcgtcatt
    22981 actattgccg cagggcaaga gcgtctcgct caaacaattt gtcagtcgcc atattaatga
    23041 gatcggtttg ctggtggtga tcgccattct ttatctggtc ttctctctga acgcgcctgg
    23101 ctttatctca ttgaataacc agatgaacgt gctgcgcgat gccgccacca tcgggatcgc
    23161 cgcatgggcg atgacgctga ttattatctc cggtgaaatt gatgtcagcg ttggtccgat
    23221 ggtggctttt gtctcggtgt gcctggcatt tttgctgcaa tttgaagttc cgctggcggt
    23281 ggcgtgtctg ctggtgttgc tgttaggcgc gctgatggga acgctcgccg gggtgctgcg
    23341 cggcgtgttt aacgtgccaa gttttgttgc cacgctgggt ttatggagcg ccctgcgcgg
    23401 aatggggctg tttatgacca acgccttgcc agtgccgatt gatgaaaacg aagtgctgga
    23461 ctggctgggg ggacaatttc tcggtgtgcc ggtatccgcg ctgatcatga tggtattgtt
    23521 tgcgctgttt gtgttcatca gccgcaaaac cgccttcggg cgctcggttt ttgccgttgg
    23581 cggtaatgcc actgcagcac agttgtgcgg tattaacgtt cgtcgggtac gcattcttat
    23641 ctttaccctt tcgggattat tagcggcggt gaccggcatt ttgttggcgg cgcgcctcgg
    23701 ttccggtaac gcaggtgccg caaacggtct ggagtttgac gtcatcgccg cggtcgtggt
    23761 tggcggtacg gcgctttccg gtggtcgcgg ctcgttgttc ggtacgctgc ttggcgtact
    23821 ggtgattacg ctaatcggta acggtctggt gctgctcggg attaactcct ttttccagca
    23881 ggtggtgcgc ggcgtcatca tcgtggtggc ggtgctggcg aatatcttgc tgactcagcg
    23941 tagcagtaaa gcgaaacgct aaaccttaac gcccctggcg cgggtgcggc agggatgaaa
    24001 attaatcgta ttgttaatga gggatttatg aaaacgatgc tggcagctta tttaccagga
    24061 aattcgaccg tcgatctgcg ggaagttgcg gtgccgacgc cggggattaa ccaggtactg
    24121 atcaaaatga aatcctccgg gatttgcgga agcgatgtcc actatatcta tcatcaacac
    24181 cgtgccacgg cagcggcacc cgataaaccg ttataccagg gatttatcaa cggtcatgaa
    24241 ccgtgcgggc agattgtggc gatggggcaa ggctgccgcc attttaaaga gggcgaccgt
    24301 gtgctggtgt atcacatttc tggctgtggt ttttgcccga actgccgtcg cggctttcct
    24361 atctcttgta ctggcgaagg aaaagcggct tacggctggc agcgtgacgg cggtcatgcc
    24421 gaatatctgc tggcggaaga aaaagatctg atcctcctgc cggatgcact gagttacgaa
    24481 gatggtgcgt ttatcagttg cggcgttggt acagcgtatg aagggatttt gcgcggcgaa
    24541 gtttccggca gtgataacgt actggtggtc ggtctggggc cggtcggcat gatggcgatg
    24601 atgctggcga aaggtcgcgg tgcaaaaagg atcatcggcg ttgatatgct gccggaacgt
    24661 ctggcgatgg cgaaacagtt aggagtgatg gatcacggct atttagcgac caccgaaggt
    24721 ctgccgcaga taatcgccga actcactcac ggcggcgcgg atgttgcgct tgattgttcc
    24781 ggtaatgccg caggtcgctt actggcactg caatccaccg ctgactgggg acgcgtggtt
    24841 tacattggtg aaaccggaaa agtcgagttc gaggttagcg ccgacctgat gcaccatcaa
    24901 cggcggatta tcggctcctg ggtgaccagt ctgttccata tggaaaaatg cgcccacgat
    24961 ttaacggact ggaaactgtg gccgcgtaac gccattaccc accgcttctc gctggaacag
    25021 gcgggagatg cttatgcact gatggcaagc ggcaaatgcg ggaaagttgt gattaacttc
    25081 ccggattaag gagttagcgg atgaccatct ataccttatc ccacgggtcg ctaaagctgg
    25141 gcgtttctga ccagggcggc gtaatcgaag gcttctggcg cgatacgacg ccgctcttac
    25201 gtcctggtaa aaaaaacggt gtggcaaccg atgcctcttg ttttccgctg gtgccatttg
    25261 caaaccgggt gagcggcaac cgttttgtct ggcaggggcg tgagtatcaa ctgcaaccga
    25321 atgttgagtg ggatgcgcac tatctgcacg gcgacggctg gctggggcag tggcaatgtg
    25381 tctcgcgcaa tgatgatatc ttgtgtctgg tgtatgaaca tcgcagcggt gtttatcact
    25441 atcgggtaag ccagacgttt catttaacgg cggatacgct gacggtgaca ctctctgtca
    25501 ccaatcaagg ggcagagacg ctgccatttg gtaccggctg gcatccttat tttccgttgt
    25561 cgccgcaaac gcggattcag gcacaggcga gcggttactg gctggagcgg gagcagtggc
    25621 tggcgggtga gttttgcgag cagctaccgc aagaattgga ttttaaccag ctcgcgccgt
    25681 tgccgcgcca gtgggtgaac aatggttttg ccggatggaa tggtcaggcc cgcatcgagc
    25741 agccgcagga gggttatgcc atcatcatag aaacgacgcc acctgcaccg tgttatttca
    25801 tctttgtttc cgacccggcg tttgataaag gatatgcgtt tgatttcttc tgtctggaac
    25861 cgatgagcca tgcgccagat gaccatcatc gcccggaagg tggtgacctc attgcgcttg
    25921 caccagggga atcaacaact tcagagatgt cgttgcgggt tgcgttactg taatttataa
    25981 aatgccggat aaggcgttca caccgcatcc ggcactcggc gcacgatgcc tgatgcgacg
    26041 cttgccgcgt cttatcaggc ctacaattgg tgttggaacg gtaggcagga taaggcgttc
    26101 acgccgcatc cggcatcttt cctaacgccg atcgagagaa atcgcccccg gcccggtaat
    26161 tgccaacagc aggaatgcgc cagcgatact gacattcttc cagaaattaa tcatatttgg
    26221 cccaaccgca tcgccggtca tatcccagta atggtgacca atcaccgccg tacccagcgt
    26281 gtagaaaata aacagcaccg ccagcggacg ggtgaaaaag ccaagcacga ttaatatcgc
    26341 ggcgggcact tccataacta ccgcaataat cgctgccagc atcggcatcg gcgcgcccag
    26401 cgaggccata tattggaccg taccgtcaaa gcccatcatt ttgggaaaac caaaaataat
    26461 gaaaattaag accacggcga tacgggcaat taataacaaa acggggcgtg cagcaccaaa
    26521 atcaaaataa cgtagtgagt tcataaccaa tccctttgca agataatgtg atttaaacgt
    26581 aatacacact cttgcacctc gccacgcccc tggcattgag atattcgcta attatcctgg
    26641 ttacagtgat ttaagcgcga tgtcctcatc tatcagcagt ttcgcatcaa acgttttgcc
    26701 gctctggatc catttgcgga ttaagcgaat ctcacgcccc tgattcagcg ttaccgcacc
    26761 gataagcacg ccgttttgca gattaaacca aatcgccttc tgagtttccg ggttgccacg
    26821 acaaatccag tcatcgccat gcatatcgcc aataaactgt aagttatcac tgtactgatc
    26881 gctccagaac cacggcggcg gcagtcgcgg aagcggtagc cccaacattg cggcagcggc
    26941 aatttgcgcc tggttattgg cgttttccca gctttcgcag cggtgtagtg caccattatc
    27001 aatacgggtg attgccacat cgccaccggc aaagatcgcg ggatcgcagg tgcggcaagc
    27061 ttcatcaatg acaataccat tggcagtatc aaggttggcc tcgcgagcca gttggtcgtt
    27121 ggcgctgata ccaataccgt aaatcaccac atcggcccga agcgtttcgc cactttgcag
    27181 cgtcagttct actttttcac catcgaccac atgttcaatg gcattattga gcagaatgcg
    27241 cacaccagcc tgctgatggc gcggtaaaag atagcgttgc acgggcggtg gtgcattacg
    27301 gcccatgacg cttgccgcca gttcaatcac tgtcacctta catccacgct gcgtggcgct
    27361 ggcagccagt tccagaccaa tagttccggc accgacaatc acgactgacc gttcgggctg
    27421 cagaacttct cgcagtctgg cggcatcacc ggcatggcgc agggtaaagc agcgttctcc
    27481 cagtgcatca agcaacggca gcggtcgagc tgccgcgccg gttgctataa aaagctgatc
    27541 ccagtgccag ctttcgccgt tagctaacac taactcttgt gtgtcgcggc ccaatgtttt
    27601 gatggttaca ccggaatgca gatgaacatt gttttcctgc caccagtgag cgggtaacac
    27661 agactgcaac tgtggggaat cttccagcaa catggatttc gagagcgggg ggcgttcata
    27721 aggaagatgt tgctcatcgg aaaacagatg cagctcaccg ctgaaccctt gctggcgtag
    27781 caaggccgca gccattgccg ccgcttgccc gccaccgaca ataatgatcg ttttttcttt
    27841 catcgtgctt ccttaaagat ccagcccagc cgctacgtgg cgaatgccgc gaatcgctaa
    27901 acccgcatca gcgttgatca tcacaccgct taatgcgcga ttattgcgcc gcgatgccaa
    27961 catcacatac ggacccgtaa aatccgccgg ttgcgggaaa aattgcagcg gtaaaatggc
    28021 ggcaattttc tccggcgtca gagactgcat tatcgaggtt tcactttgcc cgagcgcctg
    28081 tgggccgcgc aggtcgctgg ccataccaca cgggccgacg ccattcaccc gcactttcgg
    28141 tgccagttca taagccagtt ggcgaataag tccggttgcg gcatgtttac tggcggtgta
    28201 cagcgggcca ccgccgccgg gataccaggc ggcgtttgac agtgtgaaaa tcatgctgcc
    28261 ttcactggcg attaacgccg gagcgcaggc ttttgcgccc agcaggtaac cgagtacgtt
    28321 gacattaaac agctcgtgga agccggtttc gagcgtctct gcgggagtgt taaccagtga
    28381 ggcattgtga tcccagatgc ccgcattgcc gataaaacaa tccagcttgc cagaacgagt
    28441 caggatctga ttaaccgcgc gttgataatc ggcatagcag gtcacgttac cttccaccgc
    28501 cagaatatgt tcgccaaatc gctgacgcag actggcgact tttgccgccg acagttccag
    28561 cgtggcaacc tgcgcgcctt cttcgataaa tcgctcggcc agcgccaggc ctaatcccga
    28621 tccgccgccg gtaataaaga tggactcgtt atgcagatcg ctcatggctg cgcctccggt
    28681 aagtcgatga agatgtcacc accttcaacg tgtactggat aagtggttag cggatcggtg
    28741 gcgggcaggc ataacgcttt ccccgttttc aggcaaaaac tggcggcgtg cagcgggcac
    28801 tccaccgtgg cgtcatcttc cagataccct tctgacatcg acgcattacc atggctgcaa
    28861 cgatcgttaa tggcataaaa ctcgccgcct acgttgaaca gggcgatgac gggcgaggta
    28921 tcgatccgga gagcctcacc ctccggcaca tccgcaacgg gacacgcata aattcgattc
    28981 atcagaacag tacactcagg ttatgggagg agatgaccgc ttgatccagg acgatatccc
    29041 gttccagcaa gcgccagttc ccgtcctcca gacggcgaac tttgtcgaaa cgcgttccaa
    29101 catagaatgt ttcatcgcgc tctttttgtg cccgataaag tagataattc accctcacgg
    29161 caaatacgtt tgggatgtcg gtttcgctta cctggcagtt gctgattaag tgacgagtgc
    29221 gtgacggcgg ttcttctgcc caggccatgc cggtttccag acgggcgatt cgccgttcca
    29281 gctggtcttt ggtgtcatta aaaatccagg ttgtcggcgg ttgaacgcct ttgcggcggt
    29341 cgcgcgtttg tgcgttaacc gtggtgcgca tggtgtaacg aatctcttcg tcgagctgtg
    29401 ccagccagtc acgaaatttc cagtcatcca gcagactggc ttcgtgaaag agaaactggc
    29461 taatgcggtg atgtaactct agtgaaactt gcgcactcat ttcatcacct cctgctggta
    29521 agcggcggtt ttatcgagca cttcctgcca gctttcgcta ctcagaagat cggcccagcg
    29581 ttggtacatt ccacgagcgg ccgtttctga gaagatatag ttagtaatgc ctggaatgcc
    29641 gtcgtcgcga cgcttttcct gaccaagccc catttccaga cacagtttgc tgttgcgggc
    29701 gcggtggcct ttcagcaatt tctggatttc acaccagttc tccgagtcat cctgctcgag
    29761 aaaaccagca ggaccaaaag cacgagtggc gctgttttca aaagcggctt taacttcatc
    29821 ggaggcggct ttgtcagtaa tacagaacgc ccacacttcg acttgatcag ggccgcgcgg
    29881 atgccagacg cggagcgtgg cagtgccgtt gagccatgaa agcgtgggga aaatattgtt
    29941 atgacccgcc aggcgcaggg cgcgaacttc acctaaacgt tgttctgctt cggcataggt
    30001 ttcgcggtaa tagctggaaa ccgcgccatc gacccagaca ttagcatccg gtttttcagt
    30061 aaagaagaaa ccgctaccgt gaccgtcctg accaaattgc agcgcatctt tggcggtttc
    30121 ccacaccggg cgggcggttt gtccatcacc gagacgctta tcgctgccat catctttcgc
    30181 ccctaatacc tgaacggcag aagcatggct gaacagagca tgatattggt cactggcgaa
    30241 ctgctctgcc gggaatttcc agttgcagtt gatcacccac ttttgtacac cgccgacaat
    30301 ttcggtgccg ccttcgcgac gatccagcat gccatccaga taccaggcaa tgtcacccag
    30361 gtaatcacgc aggcccggtg cgctggtatc ccagttgcca aaaatcagcc ctttataact
    30421 ctccacacaa ggaacttcgt ttagtcccca gtgggattta cacaaccctt gtgggtaggc
    30481 gcgaggttcc agcggtacat cgatcaactc gccgttaatg ccataagacc agccgtgata
    30541 cggacaggta aaggcgcgag tgttaccgca atctgcataa ctcacacgca tggcccggtg
    30601 gcggcattgg ttgagaaacg ccttaatgct gccgtctttc tgacgcacta cgacaaccgc
    30661 atcttccccc atgtaggtgt taaagaaatc accatgtttt gggatctggc tttcgtgggc
    30721 gagaaatagc cagcaacgac cgaaaatgcg ctcaagctcc agttggtaaa tatccgggtc
    30781 ggtataaata cgcggagtga cccgaccgtt ttgggtgtca atcagttggt aaatgttcaa
    30841 atctgagggt gtggtcatat tttttaatcc tgaaagataa gatgtattga ccttctattt
    30901 ttgtgccata aggatgttgc tggttggttg ctgatatgtg aaatagttaa tttctaccag
    30961 ctgagacgtt ttttcgataa caaggggaag gtgatggaac tacggcattt acgctatttc
    31021 gtcgcagtgg cgcaggcact gaactttacc cgtgcggcgg aaaaactgca tacctcacag
    31081 ccttcgttaa gcagccagat ccgcgatctt gaaaactgtg tcggtgttcc gttgctggtg
    31141 agggataagc gcaaagtcgc gttgacggcg gcgggagaat gttttctcca ggacgcgctg
    31201 gcaatcctcg aacaagcgga aaatgccaaa ttacgggcgc ggaaaattgt tcaggaagac
    31261 agacaattaa ccattggctt tgtgccatcg gcggaagtga atttactgcc aaaagtatta
    31321 ccgatgtttc gtctcagaca gccagacacc ttaattgagc tggtgagttt aatcaccacg
    31381 gaacaggaag aaaaaattcg ccgtggtgaa ctcgacgtcg gcttaatgcg ccatcctgtt
    31441 tatagcccgg agattgatta tctggagctt tttgacgaac cattagtggt ggtgttaccg
    31501 gttgatcacc ctttagcaca tgaaaaagag atcactgccg cgcaactgga tggcgtgaat
    31561 ttcgtcagta ctgatccagc gtattccggt tcgcttgcgc cgatcgttaa agcgtggttt
    31621 gcgcaagaaa acagccagcc aaatatcgtc caggtggcaa cgaatattct ggtgaccatg
    31681 aatctggtgg ggatggggct gggcgtcact ttgatacccg gttatatgaa taattttaat
    31741 accggccagg tggtatttcg acccattgcc ggaaatgtgc ctgccattgc tttactgatg
    31801 gcgtggaaga aaggggagat gaagccggca ttacgcgatt tcatcgccat tgtgcaggaa
    31861 cgtttggcaa gcgtaacggc ataattacgc attatttttg caaaacgcgt cgcggatcgc
    31921 agttttttag tgcttgtgaa ctggcgttac agacatcggg aagcaggctc gaatattctt
    31981 atttttcaac ttgcaccaac attcgattaa ccccagacta acgcctcctg acgggaggga
    32041 ctcatggttt tgcaatccac gcgctggttg gcgctcggct atttcacata cttttttagt
    32101 tacggcattt ttctaccttt ctggagcgtt tggcttaagg ggattggttt aacgccagaa
    32161 accatcggcc tgttattggg ggcaggtctg gttgcccgtt ttctcgggag cttgctcatc
    32221 gcgccccgcg tcagcgatcc ttctcgcctg atttccgcct tgcgcgtgct ggcactgctg
    32281 acacttctct ttgctgtcgc cttctgggcg ggggcgcacg tagcgtggct gatgctggtg
    32341 atgattggct ttaacctctt tttctcaccg ctggtaccgt tgaccgatgc actggcgaat
    32401 acgtggcaaa agcagttccc gcttgattac ggcaaagtgc gattgtgggg ctcggtggcg
    32461 tttgtcattg gctcgtcgct gacgggcaaa ctggtcacta tgtttgatta tcgggtgatc
    32521 ctcgcgctgt tgacgttggg cgtggcatcc atgctgctcg gctttctcat ccgtccgacg
    32581 attcagccac aaggggcaag ccgccagcag gagagcaccg gttggtctgc gtggttggcg
    32641 ttggttcgcc agaactggcg ctttctggcc tgcgtttgtt tattgcaggg ggcgcatgcg
    32701 gcctattacg gttttagcgc catttactgg caggctgctg gctactcggc ctcggcggtg
    32761 ggctatttgt ggtcgctggg cgtggtggcg gaagtcatta tctttgcgct gagtaataaa
    32821 cttttccgcc gttgtagtgc acgcgatatg ctgttgatct cagcgatttg cggcgtagtg
    32881 cgctggggca ttatgggagc aactacggcg ttgccgtggt tgatagtggt gcaaattctg
    32941 cattgtggca ccttcacggt ctgccacctg gccgccatgc gctatattgc cgctcgccag
    33001 ggtagcgaag tcatccgttt acaggcggtt tactctgccg tcgcgatggg cggcagtatt
    33061 gcgatcatga ccgttttcgc cggtttcctg tatcaatatc tgggccacgg cgtgttctgg
    33121 gtgatggcgc tggtggcgct tccggcaatg tttttgcgcc cgaaagttgt tccctcatgc
    33181 tgattcgagc attttgcgga tctgctcctg ttgatgtgtt gtcaacgtca ggtccgtata
    33241 gatcagcggt ggtgaaaaga gcggtaacgg catggtataa ggagcaataa tcagtgccgc
    33301 gctgcgcgga gcgcctgtct gcaaaaatgc ctttaccgac atatgtttaa tattgagcgg
    33361 cagtaacgtt agttcgcgta gctgctgctc aatctgcgct tctcgctcgc tatcattccc
    33421 ggtcagtaga ataatctgtt tctcatgcag gtcgttttcc tgcattagcc aggcaccaaa
    33481 aatcaccgcg accagaccgc tttcctcatc agaaagatgg acgccgtatt cactttcaaa
    33541 tccggccagc gccgcgcggg tggtgcgcac caggcgtgga tacaggcggg cgaactcttc
    33601 cggcagggtg ttatcaatac cgatggcaaa caaactgcga tttaacgcct gggcgaggtg
    33661 ggtataaagc tgatcgcata acccctgttc atcataaaaa cgaacatttc ccagctcacg
    33721 aaaatggttt accagacgtt tgatagactt acgcagttgt ctgtcccgct gatgcgcatc
    33781 ccgcagtgga tcgggaaccc gcaacatgga aaaaagtaat gccataaaca gtggctcatc
    33841 aggcggtaca ggctggagcg cccgacgctg ccagtggcgt ccaatttcct gcgctacctg
    33901 aaattcaagg caggattccg cccagcgacg ttggagcgga ttaaactgag gcgtaattcc
    33961 agcgtggtgt tgcagcaaac aatattgcag atacagacac aggaaatgaa tatcgcgcgt
    34021 ttcaaagcgt ttttgcagcc gtcgggagca gagattcact aacgcttgta gattggtatc
    34081 gtcgtaaaag ttacgcgcaa ttccgcgccg cttcagttca ctcttcaggg cgggggtgaa
    34141 atggctggta ataaatgacg gacacagacg gaaaccacgt cgtagccagt gaaataaaca
    34201 caaacgttgg ttaagcactg taccttcaac ccggtagcta ccgtcataac cagtagtcag
    34261 tgtgagttga tgatagcgca ggatctcccg tcctgtttcg ctgatatcaa gactggcaat
    34321 atcatcatcc acaccattaa gctcgctgaa agttgccgtg gtggcggtca accccggctg
    34381 aaagagcgtc agcaagatct ggcagcggcg ctggggagcc gaaaggacag atggtggagc
    34441 aagcgtaggc atcatctgta gatctcccat aagcataatt gataagaata gctaaagttt
    34501 gctcagggaa atggcgaagg gaatgctaat catcagaaat gttggcaagc gtcacagaat
    34561 ttttctcatc ttaaactgtt ctgaccagtg gattagcatg tcttaaggtg gttttgtgaa
    34621 tgattatcgt gctagcgatc atgggttagt tctgcggatt caacgcgcta aggataaaat
    34681 ccaccatctg ttcgcgataa acatcgccgt gacgatcgga aaaagcatca atgtgttcgc
    34741 catccgggat taggatcaac cgttttggct cttttgccag gctatacaac ttttcgctgt
    34801 gctgccatgg gataacgtga tcagctttac cgtgaatcag taaaagcggg atcgggctga
    34861 cgctggcgat gtaattttcg ccgctgtaac tctcatcaag taagtagcca ctgccgggga
    34921 tcatctggtt ggcgatggtc gcataagagg caaatgtgga gtcgaggatc accgcacgta
    34981 tgccttcacg atcaccctga ccaataacat ccaaaatatt cgccccgcca atactctgcc
    35041 cgaacagcac cagccgttgt gggtttacat cactgcgatg gcgcaccaca ttgatggcac
    35101 tttgcgtatc gtccagcaat ccggcctggg acggcgtgcc ttttgattta ccaaacccgc
    35161 gataatcaaa cataaaaacg ttgaaattac gctcgggtaa ccaactgacc agcggccagt
    35221 gggcggacat atttccggca ttgccgtgag catgaataat ggtggcaatg gccttttcag
    35281 cggggccagt tgcagaaggg ataaaccaac catgtaggcg agtgccatct ttagcggtga
    35341 attctactga ttcggctgac cagggatcgg ggccgtagat tttgtcgtca gggtaataga
    35401 aaaaattgat ggcgatgcgc ggtattaaat aaatgcagaa ggcaacaaca aagagaataa
    35461 acagaatttt gagaacgcgt ttgttcactg gcagcgccat aacctttcct tgcgacttct
    35521 ttatcatcca ttaatcgggg aaagatggtg ggagagtggc gaaagaaagt aaagcggtga
    35581 ggaagtattt cggaatagcg cctgaaaggc gagggcgggt gagtgacatc acccgccaga
    35641 gtcattaacg cttcagggcg tcgcttaact cgtcacgcat gttcgccagc atggctttaa
    35701 caacgcgcgg gttaccagca acgatgttac cggtcagcat gtagttatga ccaccggtga
    35761 agtcgctgac gatgccgccc gcttcacgaa ccagcagctc gcctgccgcg aaatcccacg
    35821 gacgcagacc gatttcaaag aaaccgtcaa cgcgacccgc agcaacgtaa gccagatcca
    35881 gcgccgcaga accggtacga cggaagtctg cacattcgtt gaacagtttg ccgacgatgt
    35941 tgatgtaggt agtggcgtac tgttttgctt tgaacgggaa gccggtcgcc agaatagtac
    36001 cgtcgagatc gcgagcggtg ctgccgcgca gacggtagcc gttcagctgt gcgccctgac
    36061 cgcgagtggc ggtgaacagt tcgttacgca taggatcgta aaccacagca acttcagtgc
    36121 ggcctttgat acgaacggcg atagataccg cgaagtgcgg cagacgtttg ataaagttgg
    36181 tagtgccatc cagtggatcg ataacccatt gaacatcctg atcagtacct tcaagttcac
    36241 cgctttcttc ggtgatgatg gtgtgctgtg ggtaagattt acgaatcgtg tcgataatca
    36301 ccgcttcggc agctttatct acgttggtca cgaagtcgtt actgcctttc tggctcgctt
    36361 ctacagcgtc cggggtttca tagtttttgg caattaaatt acccgccttg cgcgctgcgc
    36421 gcacggcgat gttcagcatc ggatgcatcg gtctctctca ctggatgtta aagaacggga
    36481 aaacggcgca gagtatagca gcgagatcgg catctgtctt cgttttatga taagatgcgt
    36541 gaataatctt tagatgaaga aagacaatgc tgcaaaatat tcgaattgtg ctggtggaga
    36601 cgtcacacac cggcaatatg gggtctgttg cccgtgccat gaaaacaatg ggattaacca
    36661 atctgtggct ggttaatcca ctggtgaaac ctgactccca ggcgattgcc ctggcagcag
    36721 gggccagcga tgtgattggt aatgctcaca tcgtcgatac gctcgacgaa gcgttagccg
    36781 gttgtagcct ggtggtgggc accagcgcac gctcccgcac gctgccgtgg ccgatgctcg
    36841 acccgcgcga atgcggtctg aaaagcgtcg ctgaagcggc aaataccccg gtggcactgg
    36901 tgtttggtcg cgagcgcgtc ggcttgacca atgaagagct gcagaaatgc cattatcatg
    36961 tcgcgattgc ggctaacccg gaatacagtt cgctgaacct ggcgatggcg gttcaggtta
    37021 ttgcctatga agtgcgcatg gcctggctgg caactcagga aaatggcgag caggtcgagc
    37081 atgaagagac gccgtatccg ctggtcgatg atctggagcg tttttatggt catctggaac
    37141 aaacgctgct ggcaaccggt tttattcgtg aaaaccatcc ggggcaggtg atgaataaat
    37201 tgcgccgtct gtttacccgt gcgcgcccgg aaagccagga attgaatatc ctgcgcggga
    37261 ttctggcttc tatagagcag cagaataaag gtaacaaggc cgaataatag tggttgcctg
    37321 atgcgacgct taacgcgtct tatcaggcct gcagtgcaca gagcccctgg gcggataagg
    37381 cgttcacgtc gcatccgaca acaggtacaa acgccacgat aaaaaaatgg cactgaaggt
    37441 taaatacccg actaaatcag tcaagtaaat agttgaccaa tttactcggg aatgtcagac
    37501 ttgaccctgc tatgcaatac ccccactttt acaataaaaa accccgggca ggggcgagtt
    37561 tgaggtgaag taagacatga gactgacatc taaagggcgc tatgccgtga ccgcaatgct
    37621 tgacgttgcg ctcaactctg aagcgggccc ggtaccgttg gctgatattt ccgaacgtca
    37681 gggaatttcc ctttcttatc tggaacaact gttttcccgt ctgcgtaaaa atggtctggt
    37741 ttccagcgta cgtggaccag gcggtggtta tctgttaggc aaagatgcca gcagcatcgc
    37801 cgttggcgaa gtgattagcg ccgttgacga atctgtagat gccacccgtt gtcagggtaa
    37861 aggcggctgc cagggcggcg ataaatgcct gacccacgcg ctgtggcgtg atttgagcga
    37921 ccgtcttacc ggttttctca ataacattac tttaggcgaa ctggttaata accaggaagt
    37981 gctggatgtg tctggtcgtc agcatactca cgacgcgcca cgcacccgca cacaagacgc
    38041 gatcgacgtt aagttacgcg cttaataaaa agaattcaga atcaggccgg agtgctaaac
    38101 actccgtaaa cacggtcgta catccagccg gtagcctggt tccttgcatt gagtgatgta
    38161 cggagtttat agagcaatga aattaccgat ttatctcgac tactccgcaa ccacgccggt
    38221 ggacccgcgt gttgccgaga aaatgatgca gtttatgacg atggacggaa cctttggtaa
    38281 cccggcctcc cgttctcacc gtttcggctg gcaggctgaa gaagcggttg atatcgcccg
    38341 taatcagatt gccgatctgg tcggcgctga cccgcgtgaa atcgtcttta cctctggtgc
    38401 aaccgaatct gacaacctgg cgatcaaagg tgcagccaac ttttatcaga aaaaaggcaa
    38461 gcacatcatc accagtaaaa ccgaacataa agcggtactg gatacctgcc gtcagctgga
    38521 gcgcgaaggt tttgaagtca cttacctggc accgcagcgt aatggcatta tcgacctgaa
    38581 agaactcgaa gcagcgatgc gtgacgacac catccttgtt tccatcatgc acgtaaataa
    38641 cgaaatcggc gtggtgcagg atatcgcggc tatcggcgaa atgtgccgtg ctcgtggcat
    38701 tatctatcac gttgatgcaa cccagagcgt gggcaaactg cctatcgatc tgagccagtt
    38761 gaaagttgac ctgatgtctt tctccggtca caaaatctat ggcccgaaag gtattggtgc
    38821 gctgtatgtg cgtcgtaaac cgcgcgtacg tatcgaagca caaatgcatg gcggcggtca
    38881 cgagcgcggt atgcgttccg gcactctgcc tgttcaccag atcgtcggta tgggcgaagc
    38941 ctatcgcatc gcaaaagaag agatggcgac cgagatggaa cgtctgcgcg gcctgcgtaa
    39001 ccgtctgtgg aacggcatca aagatatcga agaagtttac ctgaacggtg acctggaaca
    39061 cggtgcgccg aacattctca acgtcagctt caactacgtt gaaggtgagt cgctgattat
    39121 ggcgctgaaa gacctcgcgg tttcttcagg ttccgcctgt acgtcagcaa gcctcgaacc
    39181 gtcctacgtg ctgcgcgcgc tggggctgaa cgacgagctg gcacatagct ctatccgttt
    39241 ctctttaggt cgttttacta ctgaagaaga gatcgactac accatcgagt tagttcgtaa
    39301 atccatcggt cgtctgcgtg acctttctcc gctgtgggaa atgtacaagc agggcgtgga
    39361 tctgaacagc atcgaatggg ctcatcatta atcggtatcg gaatcaggag aatttataat
    39421 ggcttacagc gaaaaagtta tcgaccatta cgagaatccg cgtaacgtgg gttcctttga
    39481 caacaacgac gagaacgtcg gcagcggcat ggtgggtgca ccggcctgtg gcgacgtgat
    39541 gaagttgcag attaaagtca acgatgaagg tatcattgaa gacgcgcgtt ttaaaactta
    39601 cggctgcggt tctgctatcg cttccagctc cctggtcacc gaatgggtga aagggaagtc
    39661 tctcgacgaa gcgcaggcga tcaaaaacac cgatattgct gaagaacttg aactgccgcc
    39721 ggtgaaaatt cactgttcta ttctggcaga agacgcgatc aaagccgcca ttgcggacta
    39781 taaaagcaaa cgtgaagcaa aataagagtt gaggtttggt tatgtcgatt acactgagcg
    39841 acagtgcagc agcgcgagta aatacctttc tggctaaccg cggcaaaggg tttggcctgc
    39901 gtctgggcgt gagaacctcc ggatgttcag gtatggctta tgtactggaa tttgttgacg
    39961 aaccgacgcc ggaagacatc gtgtttgaag acaaaggcgt gaaagtcgtg gtcgatggca
    40021 aaagcctgca atttctggac ggtacgcagc tggacttcgt aaaagaaggc ctgaacgaag
    40081 ggtttaaatt caccaacccg aacgtcaaag atgagtgtgg ttgcggcgaa agcttccacg
    40141 tttgatgcgc ataccgataa ccccaccgtg gtcgcctgcg cgtggggttt gttttacctg
    40201 attcgccgtt atcgcggcgg atcgcagccc tgagaatgtt atggattact tcaccctctt
    40261 tggcttgcct gcctgttatc aactcgatac ccaggcgctg agcctgcgtt ttcaggatct
    40321 acaacgtcag tatcatcctg ataaattcgc cagcggaagc caggcggaac aactcgccgc
    40381 cgtacagcaa tctgcaacca ttaaccaggc ctggcaaacg ctgcgtcatc cgttaatgcg
    40441 cgcggaatat ttgctttctt tgcacggttt tgatcttgcc agcgagcagc atactgtgcg
    40501 cgacaccgcg ttcctgatgg aacagctgga actgcgcgaa gaactggacg agatcgaaca
    40561 ggcgaaagat gaagcgcggc tggaaagctt tatcaaacgt gtgaaaaaga tgtttgatac
    40621 ccgccatcag ttgatggttg aacagttaga caacgagacg tgggacgcgg cggcggatac
    40681 cgtgcgtaag ctgcgttttc tcgataaact gcgaagcagt gccgaacaac tcgaagaaaa
    40741 actgctcgat ttttaatttc tggaagctaa acatggcctt attacaaatt agtgaacctg
    40801 gtttgagtgc tgcgccgcat cagcgtcgtc tggcggccgg tatcgacctt ggcacaacca
    40861 actcgctggt ggcgacagtg cgcagcggtc aggccgaaac gttagccgac catgaaggcc
    40921 gtcacctgct gccgtctgtt gttcactatc aacagcaagg gcattcggtg ggttatgatg
    40981 cgcgtactaa cgcagcgctc gataccgcca acaccattag ttctgttaaa cgcctgatgg
    41041 ggcgctcgct ggctgatatc cagcaacgct atccacatct gccttatcaa ttccaggcca
    41101 gcgaaaacgg cctgccgatg attgaaacgg cggcggggct gctgaacccg gtgcgcgttt
    41161 ctgcggacat cctcaaagca ctggcggcgc gggcaactga agccctggca ggcgagctgg
    41221 atggtgtagt tatcaccgtt ccggcgtact ttgacgatgc ccagcgtcag ggcaccaaag
    41281 acgcggcgcg tctggcgggg ttgcatgttc tgcgcttact taacgaaccg accgctgcgg
    41341 ctatcgccta cgggctggat tccggtcagg aaggcgtgat cgccgtttat gacctcggcg
    41401 gcgggacgtt tgatatttcc attctgcgct taagtcgcgg cgtgtttgaa gtgctggcga
    41461 ccggcggtga ttccgcgctc ggcggcgatg atttcgacca tctgctggcg gattacattc
    41521 gcgagcaggc gggcattcct gatcgtagcg ataaccgcgt tcagcgtgaa ctgctggatg
    41581 ccgccattgc agccaaaatc gcgctgagcg atgcggactc cgtgactgtg aatgttgcgg
    41641 gctggcaggg cgaaatcagc cgtgaacaat tcaacgaact gatcgcgcca ctggtaaaac
    41701 gaaccttgct ggcttgtcgt cgcgcgctga aagacgctgg cgtagaagct gatgaagtgc
    41761 tggaagtggt gatggttggc ggttctactc gcgtgccgct agtgcgtgaa cgagtaggcg
    41821 aatttttcgg tcgtccaccg ctgacttcta tcgacccgga taaagtcgtc gctattggcg
    41881 cggcgattca ggcggatatt ctggtgggta acaagccgga cagcgaaatg ctgttgctgg
    41941 atgtgatccc gctgtcgctg ggcctcgaaa cgatgggcgg cctggtggag aaagtgattc
    42001 cgcgtaatac cactattccg gtggcgcgcg ctcaggattt caccaccttt aaagatggtc
    42061 agacggcgat gtctatccat gtaatgcagg gtgagcgcga actggtgcag gactgccgct
    42121 cgctggcgcg ttttgcgctg cgtggtattc cggcgttacc ggctggcggc gcgcatattc
    42181 gcgtgacgtt ccaggtcgat gccgacggtc ttttgagcgt gacggcgatg gagaaatcca
    42241 ccggcgttga ggcatctatt caggtcaaac cgtcctacgg tctgaccgac agcgaaatcg
    42301 cctcgatgat caaagactca atgagctatg ccgagcagga cgtaaaagcc cgtatgctgg
    42361 cagaacaaaa agtagaagcg gcgcgtgtgc tggaaagttt gcacggcgcg ctggctgctg
    42421 atgccgcgct gttaagcacc gcagagcgtc aggtcattga cgatgctgcc gctcacctga
    42481 gtgaagtggc gcagggcgat gatgttgacg ccatcgaaca agcgattaaa aacgtagaca
    42541 aacaaaccca ggatttcgcc gctcgccgca tggaccagtc ggttcgtcgt gcgctgaaag
    42601 gccattccgt ggacgaggtt taatatgcca aagattgtta ttttgcctca tcaggatctc
    42661 tgccctgatg gcgctgttct ggaagcgaat agtggtgaaa ccattctcga cgttgcgctg
    42721 cgtaacggta tcgagattga acacgcctgt gaaaaatcct gtgcttgcac cacctgccac
    42781 tgcatcgttc gcgaaggttt tgactcgctg ccggaaagct cagagcagga agacgacatg
    42841 ctggacaaag cctggggact ggagccggaa agccgtttaa gctgccaggc gcgcgttacc
    42901 gacgaagatt tagtggtcga aatcccgcgt tacactatca accatgcgcg tgagcattaa
    42961 cagaggttag tatgggactt aagtggaccg atagccgcga aattggcgaa gcactgtacg
    43021 atgcgtatcc cgatcttgat ccgaaaacgg ttcgattcac cgatatgcat cagtggattt
    43081 gcgatctgga agatttcgac gacgacccgc aggcatcgaa cgagaaaatc ctcgaagcga
    43141 ttttgttggt ctggctggac gaagccgagt gaatgaacgg gctgcctggg ggtagcccgt
    43201 tttgctgtta aacgaataag gataactaaa atgacagaag cgatgaaaat taccctttct
    43261 acccaacctg ccgacgcgcg ctggggagaa aaagcgactt acagcattaa taatgatggc
    43321 attaccctgc atttgaacgg gacagacgat ctggggctga tccagcgtgc ggcgcgcaag
    43381 attgacggtc tgggcatcaa gcatgttcag ttaagcggtg aagactggga tgcggatcgc
    43441 tgctgggcat tctggcaagg ttacaaagcc ccgaaaggca cacgtaaagt ggagtggccg
    43501 gatctggacg atgcccaacg gcaggaactg gataaccgcc tgatgatcat cgactgggtg
    43561 cgtgacacca tcaacgcacc ggcggaagaa ctggggccat cgcaactggc acagcgtgct
    43621 gttgatctga tcagcaacgt cgcgggcgat cgtgtgactt accggatcac caaaggcgaa
    43681 gatctgcgtg atcaaggtta tatggggctg cacacggtcg ggcgcggttc agaacgttcc
    43741 ccggtattgc tggcgctgga ttacaaccca acaggcgata aagaagcgcc agtgtacgcg
    43801 tgcctggttg gtaaaggcat cacctttgat tccggcggct acagtatcaa acagaccgca
    43861 tttatggact cgatgaagtc ggacatgggc ggcgcggcaa cggttaccgg agcgctggcg
    43921 tttgccatta cgcgcggact gaacaagcgc gtgaagctgt tcctctgctg tgcggataac
    43981 ctgatcagcg gcaatgcgtt caagctgggc gatatcatta cctatcgcaa cggtaaaaaa
    44041 gttgaagtga tgaacaccga tgcggaaggg cgtctggtgc tggccgatgg cctgattgat
    44101 gccagtgcgc agaaaccgga actgatcatt gatgcggcga ccctcaccgg ggcggcgaaa
    44161 accgcgctgg gtaatgatta tcacgcgctg ttcagttttg acgatgcgct tgccgctcgc
    44221 ttgctggcga gtgccgcgca ggagaatgaa ccattctggc gtctgccgct ggcggagttc
    44281 caccgcagcc agctgccgtc taactttgcc gaactgaaca ataccggaag cgcggcgtat
    44341 ccggcaggtg caagcacggc ggcgggcttc ctgtcgcact ttgttgagaa ctatcagcaa
    44401 ggctggctgc atatcgactg ctcggcgact taccgtaaag cgccggttga acagtggtct
    44461 gcgggcgcga ccgggctagg tgtgcgcacg attgctaatc tgttaacggc gtaaacgtct
    44521 tatttggcct acaaaattgc gaaaatccaa tagattgaga ttcccttgta ggcctgataa
    44581 gcgtagcgca tcaggcactt gtgccccgaa cgttgaatga attgtggttc ttatgtccga
    44641 aacaaaaaac gaacttgaag acctgctgga aaaagcagca accgaaccgg cgcaccgccc
    44701 ggcctttttc cgtactctac tggaatccac cgtctgggtg ccgggtacgg cggcgcaggg
    44761 cgaggctgtg gttgaagata gcgcgcttga tttacagcac tgggaaaaag aagacggcac
    44821 cagcgtcatt ccttttttta cctcgttaga agcacttcaa caggcggttg aagacgaaca
    44881 ggcatttgtc gtaatgcccg ttcgcacatt gtttgagatg acccttggcg aaacgctctt
    44941 ccttaatgcc aaactgccaa ccggtaaaga atttatgccg cgtgaaatca gtttgctgat
    45001 tggtgaagaa ggaaatccgc tgagcagcca ggaagtactg gaaggcggtg aatcgctgat
    45061 attatcggaa gtcgcagagc caccagcaca aatgattgat tcactcacca ccttatttaa
    45121 aactattaag ccggtgaagc gtgcttttat ttgttcaatt aaagagaacg aagaggcaca
    45181 gcctaattta cttattggca ttgaagccga tggtgatatc gaagaaatta ttcaggcggc
    45241 gggaagtgta gcgaccgata cattacctgg cgatgaaccc atcgatattt gtcaggtgaa
    45301 aaaaggggaa aaaggaatta gccactttat taccgaacat attgcgccat tctatgaacg
    45361 tcgctggggt ggttttttgc gtgactttaa acagaaccgg ataatctaaa atatgccgcc
    45421 ccaaagggcg gcattcgggc ttacttgctg ctctgaaggt tgttaaccgc cagtaaattg
    45481 ttgccggaga gactccggtt attatttacc tcgcttaaca gcgcaccaga aacaataagt
    45541 acgatacaaa agcaccgtat cttcattgtg cggtccatgc gaagaccgca tcacgacagc
    45601 accactggcg atattgccgc gatagcaagc gctgagacgc gcttgctcta acggtcgcgg
    45661 aacatccttt ccttaagcca gcgcccgctt gcgagacggc ggaaaggtcc gcagtggata
    45721 atacactaac agaacaataa taactctctt ttacttgaca aaaagagagt tactggcgag
    45781 tattgtgtcc gctgagacgc gcttgcatta ctacggtaat acaatctgct ggaaaggccg
    45841 cgaaccaaac cagcaacaaa taaccgcctg attttggcgg ttttttgttt tttaaaggaa
    45901 agtgaattta catgtttaag taat
//
LOCUS       DAZUCC010000012        45762 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZUCC010000012 DAZUCC010000000
VERSION     DAZUCC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.012"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(363..449)
                     /locus_tag="ACHY09_000576"
     tRNA            complement(363..449)
                     /locus_tag="ACHY09_000576"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(413..415),aa:Leu,seq:gag)
     gene            complement(464..796)
                     /gene="secG"
                     /locus_tag="ACHY09_000577"
     CDS             complement(464..796)
                     /gene="secG"
                     /locus_tag="ACHY09_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS3883593.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQIQPAAPA
                     KPTSDIPN"
     gene            complement(1024..2361)
                     /gene="glmM"
                     /locus_tag="ACHY09_000578"
     CDS             complement(1024..2361)
                     /gene="glmM"
                     /locus_tag="ACHY09_000578"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS3883594.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2354..3202)
                     /gene="folP"
                     /locus_tag="ACHY09_000579"
     CDS             complement(2354..3202)
                     /gene="folP"
                     /locus_tag="ACHY09_000579"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFS3883595.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYHDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3292..5226)
                     /gene="ftsH"
                     /locus_tag="ACHY09_000580"
     CDS             complement(3292..5226)
                     /gene="ftsH"
                     /locus_tag="ACHY09_000580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS3883596.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5326..5955)
                     /gene="rlmE"
                     /locus_tag="ACHY09_000581"
     CDS             complement(5326..5955)
                     /gene="rlmE"
                     /locus_tag="ACHY09_000581"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFS3883597.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6081..6374
                     /gene="yhbY"
                     /locus_tag="ACHY09_000582"
     CDS             6081..6374
                     /gene="yhbY"
                     /locus_tag="ACHY09_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS3883598.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6530..7006)
                     /gene="greA"
                     /locus_tag="ACHY09_000583"
     CDS             complement(6530..7006)
                     /gene="greA"
                     /locus_tag="ACHY09_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS3883599.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7254..8687
                     /gene="dacB"
                     /locus_tag="ACHY09_000584"
     CDS             7254..8687
                     /gene="dacB"
                     /locus_tag="ACHY09_000584"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFS3883600.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8728..9900)
                     /gene="cgtA"
                     /locus_tag="ACHY09_000585"
     CDS             complement(8728..9900)
                     /gene="cgtA"
                     /locus_tag="ACHY09_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFS3883601.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9916..10881)
                     /gene="yhbE"
                     /locus_tag="ACHY09_000586"
     CDS             complement(9916..10881)
                     /gene="yhbE"
                     /locus_tag="ACHY09_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS3883602.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLGPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11008..11265)
                     /gene="rpmA"
                     /locus_tag="ACHY09_000587"
     CDS             complement(11008..11265)
                     /gene="rpmA"
                     /locus_tag="ACHY09_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS3883603.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11286..11597)
                     /gene="rplU"
                     /locus_tag="ACHY09_000588"
     CDS             complement(11286..11597)
                     /gene="rplU"
                     /locus_tag="ACHY09_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS3883604.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11856..12827
                     /gene="ispB"
                     /locus_tag="ACHY09_000589"
     CDS             11856..12827
                     /gene="ispB"
                     /locus_tag="ACHY09_000589"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFS3883605.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13055..13333
                     /gene="sfsB"
                     /locus_tag="ACHY09_000590"
     CDS             13055..13333
                     /gene="sfsB"
                     /locus_tag="ACHY09_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFS3883606.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13381..14640)
                     /gene="murA"
                     /locus_tag="ACHY09_000591"
     CDS             complement(13381..14640)
                     /gene="murA"
                     /locus_tag="ACHY09_000591"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS3883607.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14695..14949)
                     /gene="ibaG"
                     /locus_tag="ACHY09_000592"
     CDS             complement(14695..14949)
                     /gene="ibaG"
                     /locus_tag="ACHY09_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFS3883608.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15109..15402)
                     /gene="mlaB"
                     /locus_tag="ACHY09_000593"
     CDS             complement(15109..15402)
                     /gene="mlaB"
                     /locus_tag="ACHY09_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFS3883609.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15402..16037)
                     /gene="mlaC"
                     /locus_tag="ACHY09_000594"
     CDS             complement(15402..16037)
                     /gene="mlaC"
                     /locus_tag="ACHY09_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFS3883610.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16056..16607)
                     /gene="mlaD"
                     /locus_tag="ACHY09_000595"
     CDS             complement(16056..16607)
                     /gene="mlaD"
                     /locus_tag="ACHY09_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFS3883611.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16612..17394)
                     /gene="mlaE"
                     /locus_tag="ACHY09_000596"
     CDS             complement(16612..17394)
                     /gene="mlaE"
                     /locus_tag="ACHY09_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFS3883612.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17402..18211)
                     /gene="mlaF"
                     /locus_tag="ACHY09_000597"
     CDS             complement(17402..18211)
                     /gene="mlaF"
                     /locus_tag="ACHY09_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFS3883613.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18421..19398
                     /gene="yrbG"
                     /locus_tag="ACHY09_000598"
     CDS             18421..19398
                     /gene="yrbG"
                     /locus_tag="ACHY09_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFS3883614.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19412..20398
                     /gene="kdsD"
                     /locus_tag="ACHY09_000599"
     CDS             19412..20398
                     /gene="kdsD"
                     /locus_tag="ACHY09_000599"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFS3883615.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGVTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20419..20985
                     /gene="kdsC"
                     /locus_tag="ACHY09_000600"
     CDS             20419..20985
                     /gene="kdsC"
                     /locus_tag="ACHY09_000600"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFS3883616.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSIAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            20982..21557
                     /gene="lptC"
                     /locus_tag="ACHY09_000601"
     CDS             20982..21557
                     /gene="lptC"
                     /locus_tag="ACHY09_000601"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFS3883617.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21526..22083
                     /gene="lptA"
                     /locus_tag="ACHY09_000602"
     CDS             21526..22083
                     /gene="lptA"
                     /locus_tag="ACHY09_000602"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFS3883618.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22090..22815
                     /gene="lptB"
                     /locus_tag="ACHY09_000603"
     CDS             22090..22815
                     /gene="lptB"
                     /locus_tag="ACHY09_000603"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFS3883619.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22863..24296
                     /gene="rpoN"
                     /locus_tag="ACHY09_000604"
     CDS             22863..24296
                     /gene="rpoN"
                     /locus_tag="ACHY09_000604"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS3883620.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24319..24606
                     /gene="hpf"
                     /locus_tag="ACHY09_000605"
     CDS             24319..24606
                     /gene="hpf"
                     /locus_tag="ACHY09_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFS3883621.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24724..25215
                     /gene="ptsN"
                     /locus_tag="ACHY09_000606"
     CDS             24724..25215
                     /gene="ptsN"
                     /locus_tag="ACHY09_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFS3883622.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25261..26115
                     /gene="rapZ"
                     /locus_tag="ACHY09_000607"
     CDS             25261..26115
                     /gene="rapZ"
                     /locus_tag="ACHY09_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS3883623.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26112..26384
                     /gene="npr"
                     /locus_tag="ACHY09_000608"
     CDS             26112..26384
                     /gene="npr"
                     /locus_tag="ACHY09_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFS3883624.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26597..27229
                     /gene="yrbL"
                     /locus_tag="ACHY09_000609"
     CDS             26597..27229
                     /gene="yrbL"
                     /locus_tag="ACHY09_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFS3883625.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27226..27954)
                     /gene="mtgA"
                     /locus_tag="ACHY09_000610"
     CDS             complement(27226..27954)
                     /gene="mtgA"
                     /locus_tag="ACHY09_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFS3883626.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(27951..28604)
                     /gene="elbB"
                     /locus_tag="ACHY09_000611"
     CDS             complement(27951..28604)
                     /gene="elbB"
                     /locus_tag="ACHY09_000611"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFS3883627.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28834..31170)
                     /gene="arcB"
                     /locus_tag="ACHY09_000612"
     CDS             complement(28834..31170)
                     /gene="arcB"
                     /locus_tag="ACHY09_000612"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFS3883628.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLALVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31266..32195)
                     /gene="yhcC"
                     /locus_tag="ACHY09_000613"
     CDS             complement(31266..32195)
                     /gene="yhcC"
                     /locus_tag="ACHY09_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS3883629.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQTECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            32870..37330
                     /gene="gltB"
                     /locus_tag="ACHY09_000614"
     CDS             32870..37330
                     /gene="gltB"
                     /locus_tag="ACHY09_000614"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFS3883630.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37343..38761
                     /gene="gltD"
                     /locus_tag="ACHY09_000615"
     CDS             37343..38761
                     /gene="gltD"
                     /locus_tag="ACHY09_000615"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFS3883631.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            38944..40071
                     /gene="yhcG"
                     /locus_tag="ACHY09_000616"
     CDS             38944..40071
                     /gene="yhcG"
                     /locus_tag="ACHY09_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFS3883632.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNPDARSFYE
                     KETLRCGWSVRQLERQITTQFYERTLLSHDKSAMLQQHAPAEMHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(40131..40595)
                     /gene="nanQ"
                     /locus_tag="ACHY09_000617"
     CDS             complement(40131..40595)
                     /gene="nanQ"
                     /locus_tag="ACHY09_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFS3883633.1"
                     /translation="MIMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQCDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(40592..41467)
                     /gene="nanK"
                     /locus_tag="ACHY09_000618"
     CDS             complement(40592..41467)
                     /gene="nanK"
                     /locus_tag="ACHY09_000618"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFS3883634.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALHDA
                     LSALVSPLQVHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHCSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(41464..42153)
                     /gene="nanE"
                     /locus_tag="ACHY09_000619"
     CDS             complement(41464..42153)
                     /gene="nanE"
                     /locus_tag="ACHY09_000619"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS3883635.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(42201..43691)
                     /gene="nanT"
                     /locus_tag="ACHY09_000620"
     CDS             complement(42201..43691)
                     /gene="nanT"
                     /locus_tag="ACHY09_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFS3883636.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(43801..44694)
                     /gene="nanA"
                     /locus_tag="ACHY09_000621"
     CDS             complement(43801..44694)
                     /gene="nanA"
                     /locus_tag="ACHY09_000621"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFS3883637.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(44816..45607)
                     /gene="nanR"
                     /locus_tag="ACHY09_000622"
     CDS             complement(44816..45607)
                     /gene="nanR"
                     /locus_tag="ACHY09_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFS3883638.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        11225 a        12443 c        11419 g        10675 t
ORIGIN      
        1 aaacactgtt agttttttct ttttaaatga tgcggccatt gttctcagta attaaataac
       61 attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg
      121 agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact ggagttgaca
      181 caataaagtg ccaattatgt cagcagaagg gaaaagttta taactaaggc gtaccgatac
      241 catcgtttca aagtaccagc tacgagtaaa gcaactggac gagatacaga tacctgacaa
      301 ccattcctca gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa
      361 tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt
      421 agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc gggatatcgc
      481 tggtcggctt agccggagct gctggctgaa tttgttcggt tttcgccggc gcactcaggt
      541 tttcccattc gctaccttta ttggttttgt tgctattgat gttacccagc accagactga
      601 tgatgaagaa taacgttgcc agcagcgctg tcatgcgggt catgaagtta ccagaaccac
      661 ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt
      721 taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacta
      781 aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa
      841 ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac taaccaaagc
      901 gaatgacctt cgcaagggca attttattgc attgtatcaa ctgcggaaaa aaacagcaaa
      961 accgatgtgt tcgctgagaa aaaaggcgac aatcgccgcc tttttagcca gttatttatc
     1021 gttttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc
     1081 gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac gcaacagcac
     1141 acgtccacgg ctacccagcg cagcttcaac ttctgcggtc acagctttga ctgactcatg
     1201 ctccagcgga tcgccgctgc ctgctgtgta acgcacgtta accagaatct gcgggaacat
     1261 tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgcgccatcg ccgccagcac
     1321 ctgcaagcca gcaacaatgc cgtcaccggt agtggtttta tccagcagga tcacatgacc
     1381 ggagttctct gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc
     1441 acccactttc gcgcgcgcaa atggaatacc taactgtttc agcgccagtt caagccccat
     1501 gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa gaccttcacg
     1561 cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt caaccataat
     1621 cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg
     1681 agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgttcgg
     1741 ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg gcgcgatgtg
     1801 ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc taagttcgtt
     1861 cgggaacgtg gctttgcaaa actcgatata gcgacccgca gcatcaacga tacggctggc
     1921 tttacccagt tctgccgaat caacgcagct gatctctttt tccatttccg cttcgatggc
     1981 ctcttctacc gcatccggta gtttggtgcc gtcgatagag aagaatttaa tgccgttatc
     2041 gtagaatggg ttatgcgatg cagaaattac aattccagcc tctgcgcgga aggtacgcgt
     2101 cagataagcc acggccggtg ttggcatcgg gccagtgaag agcgcggaaa gaccggctgc
     2161 cgcaagcccc gcttccagtg ctgactccag catatagcca gaaatacgcg tgtctttacc
     2221 aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt
     2281 aagcacaaaa tcaggggtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc
     2341 gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg gcttccacca
     2401 cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc gcgccttgca
     2461 ttgcggcaat gaccgcacag gccagactac cgctcaaacg ctcggacggc cccacgttca
     2521 gcagctgtcc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt
     2581 gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga
     2641 atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt
     2701 gctcaataaa gtagcgattc acttctgcaa agacatcgtg atacttcggg gcttcctgca
     2761 tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagccg
     2821 cctccagagc gccaggttcg gaaagggagc ggatatcgtt aataatgtga gcgccaactt
     2881 tcgctgactc acggatgact tctggtttgg atgtatctac cgagatccag acttcgaagc
     2941 gttgagcaat tgcctcaacc acaggaataa cacgttgtaa ctcttcttca acgctaactt
     3001 ccgccgcccc tggacgtgtg gactcgccac caacgtcaat gatcgtcgcg ccagcgttga
     3061 tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa
     3121 aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag
     3181 taccctgggc aaagagtttc ataatgttat ccctggtatg aattgataag aaaaaacccc
     3241 ggagcacgcc ccggggtttt tggtacaaat acagtcatct gatgcgggaa cttacttgtc
     3301 gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg
     3361 aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt cttcccagcc
     3421 cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt
     3481 ctcatatttc atgagagcat ctttcatcgc atgcagtata tccatattgt cggtcagaag
     3541 ctgacgcgca cgattatagt tgcgctcaat cagtgctttc acttcctggt cgatgatacg
     3601 tgcagtttca tcggacatat gtttcgcctt cgctacgcta cggccgagga acacttcacc
     3661 ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat
     3721 gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag aaacatgttc
     3781 cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa tctggctttc
     3841 cagtttctga cggctagcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag
     3901 cgcacgaccg cgtgggataa tcgtcacttt gtgcaccgga tcatgttccg gcaccaggcg
     3961 accgataatc gcatgacccg cttcgtggta agccgtcgat tctttctgcg cttccgtcat
     4021 caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct cgaactcaac
     4081 catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt
     4141 cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc
     4201 gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgttcacgac cgcgaacatc
     4261 tggcaagcca accacaacct gacggtcgaa acggccagga cgcagcagcg ccgggtcgag
     4321 aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt cgaagccatc
     4381 catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccagacc
     4441 tgcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggtgccgc
     4501 tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc
     4561 tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caatcgcttt
     4621 cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt tcgggatctt
     4681 accgccgagt ttctggaagc ggctcggctc gcgcagatac tcaaccagtt cagcaacttc
     4741 ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct gatcttcagt
     4801 cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc cgccctgcat
     4861 ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc aggagatgaa
     4921 gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga cgttcttggt
     4981 caacaagtta tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact
     5041 atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt
     5101 attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc
     5161 aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt
     5221 cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat
     5281 actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc
     5341 ggtcgctaca atatacactt cccgcgaacg tgcgcgagaa gagtccggct tacgaacttt
     5401 gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa
     5461 caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt ctagcgccag
     5521 ttccaccaga tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc
     5581 catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat
     5641 caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg
     5701 taaaagatcg caagcgatga tacggccttt accgccaatt tgggtgacca catattgtga
     5761 ccaaccaccc ggcgcagcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc
     5821 actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc
     5881 ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg
     5941 cttcttacct gtcatttaac tttcccattg ggataactca tcgtaaccaa ttgcgtaaat
     6001 ttttacgcgc ctatttggcg atatatggga gatggcggta gaatgacccg ttttcaatcc
     6061 caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc
     6121 tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc
     6181 tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg
     6241 aagatcgcga aactaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta
     6301 atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct
     6361 cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt
     6421 tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gcatagcggc
     6481 ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct tacaggtatt
     6541 ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc acaacatcat
     6601 cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc aggttttgtt
     6661 taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca gaatccagat
     6721 tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt ttggtgacat
     6781 caatcacctg cgcgttcgac agcttggctt cgatgtcttt gatacggcct tcgcagaagc
     6841 cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca tgctcacgcg
     6901 cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa tccagctctt
     6961 cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt tatacctctt
     7021 gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc ccggcataac
     7081 tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca ggtcagctac
     7141 aattcacatt ttgatagtca ttttaccctg aagttcccga agggtcatcg tttactttat
     7201 agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag attatgcgat
     7261 tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg
     7321 ttgatgagta cattactcaa ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag
     7381 tcggcgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg cccgccagta
     7441 cgcagaaagt gattactgca ctggcggcgt tgattcaact cggccccgat tttcgtttta
     7501 ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg
     7561 cgcgatttgg tgccgatccg acgttaagac gtcaggatat tcgcaatatg gtcgcgactt
     7621 tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc tccattttcg
     7681 ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc tttagcgctc
     7741 cgcctgccgc cgccatagtt gaccgcaact gtttctccgt ctcgctctat agtgccccaa
     7801 aacctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg atgttcagcc
     7861 aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgagctg gatgtggtgc
     7921 caggcgacct gaaccgcttt acgctgaccg gatgcctgcc acaacgttct gagccgctcc
     7981 cgttggcttt tgccgtgcag gatggtgcca gctatgctgg agcaattctg aaagatgagt
     8041 taaaacaggc aggtatcacc tggagcggaa cactgctgcg tcagacgcag gttaacgaac
     8101 ccggaacggt agttgccagt aaacagtcgg ccccgttgca cgatctgctt aagattatgc
     8161 tgaaaaagtc ggacaacatg attgccgata cggttttccg catgatcggc catgcgcgct
     8221 tcaatgtgcc tggaacatgg cgggccgggt cggacgccgt gcgtcagatc ctgcgccagc
     8281 aagccggtgt cgatattgga aataccatta ttgccgatgg ttcagggctt tcgcggcata
     8341 acctgattgc tcccgccacc atgatgcagg tgctgcaata cattgcccaa cacgacaatg
     8401 aacttaactt tatctctatg ctgccgctgg cgggctatga cggctctttg cagtaccgtg
     8461 caggtctgca tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg
     8521 gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg gcgtttgtgc
     8581 aatatctttc tggctatgca gtagaaccgg cggatcagcg taatcgccgt attccgttgg
     8641 tacgttttga aagccgtttg tataaggata tttaccagaa caattagtct aaagagaaac
     8701 cccggcacat ggccggggtt ttgctgatta acgcttgtaa atgaactcaa cgccttcttc
     8761 gtcgtcttcg tcccagtcgt catcccagtc ttcatcatcc tcttcagcaa tctcttcaag
     8821 ctgctggcga tgataatcat cccacatgaa ttcgactttc tctggctgtt tcgcttcttc
     8881 agcctgcacg accgggtttt caatgataaa ggtcatcaca tcccagcaga gatctttcac
     8941 gcccagtccg ctcgccgcag agatcagata atatttatct tcccaaccca gcgcctcagc
     9001 gatcgctttc gctttctctt cggcttctgc cttatccagc aggtcgatct tgttgaagac
     9061 taaccaacgc ggtttcgccg ccagatcctg gctgtatttt tccagctcgc tgataataat
     9121 acgcgcgttt tcaaccggat cggtgccatc aatcggatcg atatcgatga ggtgcaacag
     9181 gacgcggcaa cgctccaggt gcttcaggaa gcgaatgccc agacctgcgc cttctgcagc
     9241 gccttcaatc agtcctggaa tatcggcaac aacgaagctc ttttcgttgt ccattcgtac
     9301 cacaccaaga cttggcacca gagtggtaaa cggataatcc gccactttcg gtttagccgc
     9361 cgatactgcg cggataaatg tcgatttacc cgcgtttggc atccccagca taccgacgtc
     9421 agccagcagc atcagctcca gtagcagctc acgcttatcg cccggcgtac cgttagtttt
     9481 ctgccgtggt gtacggttaa ctgacgattt gaaacgggta ttgcccagac cgtgccagcc
     9541 gcccttagca accagcagac gctgaccgtg tttggtcata tcgcccatgg tttcaccggt
     9601 accctggtcg ataacacgcg tacctaccgg cactttaatc gtcacgtctt taccgcgctt
     9661 accggtacag tcgcggcttg cgccattctg accacgctct gcgcggaaag atttttcaaa
     9721 acgataatcg ataagcgtgt tcaggttctc gtcggcttcc atccatacgt caccgccgtc
     9781 accgccgtcg ccgccatccg ggccgccttt cggaatatac ttttcgcggc ggaagctcac
     9841 gcaaccatta ccgccatcac ctgcaacgac cagaatcgat gcttcatcaa caaacttcat
     9901 tttattctcc gtaaatcatt cgcctgcgcg gggttgcgat accaccgttg tatgcttacg
     9961 taatccgccc caaatacgat gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa
    10021 cgcaccgaga taacctaaaa ggtttaacat cggtctggcg aagaaatcgg gccaggccag
    10081 tgataaaaga tctgaaaaaa acagcgtaaa cagtggggtg agcgtaatga tcgcgctcac
    10141 ctgcgctgcc tgccagcgag ccatcgcttc cgccagggcg ccatatccta ccaatgtatt
    10201 cagtccgcaa aaaattaaac atgcgagctg ccagtggcta agctgcgcta tcactccagg
    10261 ctttgccaga gggaagagcg caattgtaca taaagtgtac agcaaaaaca ggatctgcgg
    10321 tgaggccagt cgacgcaata aaaccttttg cgccacgcca taactcaccc acaccgtcgc
    10381 cgcaccgacc ccaaagatta ctccccaggt gtaatcggtg agctttgtaa atatctcgac
    10441 cagactggtg ttaaaaaaca tcaccaggcc gctcaggagc atcaatgccc ctacaacctg
    10501 agtgctgcgc attttctctt tcaggataaa tacgctggca accatcatgc caactggcga
    10561 gagttgccca atcacctgcg aagcggtcgg acccaggtat tgcaaggatg agctgaacag
    10621 gatgaagttc ccaaacagcc cggcggtcgc cactgccaac aaaatcaacc agcgtggctt
    10681 acgaaacacg cgtaatggcg gcaaccgctt cttcaccgca agaatggcac ccaggccaat
    10741 actcgccatc aagaaacggt aaaacacgat tgtcggaggt tccatcacct ccagcacctg
    10801 cttcattgcg attggcaacg ccccccagca aattgctgtg gtgagcgcca aaagaatgcc
    10861 aatgcctgcc tgctgcttca taccgtattc cctgcaaagc gcatttgcgg attaacgtcg
    10921 caccatgcgc atttttaccg gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct
    10981 ttcatccgtt accgggacgc gaaaaactta ttcagcttcg atgctgataa atttacggtt
    11041 tttcgggcct ttaacttcga atttcacttt accgtctgct ttagcaaaca gagtgtggtc
    11101 acgaccgcaa cctacgttag cgccagcgtg gaatttggta ccacgttgac gaacgatgat
    11161 gctacccgcc agaacggatt cgccaccgaa acgcttaacg cccaggcgtt tagcttctga
    11221 atcgcgaccg ttacgtgtgg agccgccagc ctttttatgt gccatttgaa atctctcctc
    11281 aggtcttagg cgctgatgcc agtaattttc acatcagtga accactgacg atggccctgc
    11341 tgcttacgat agtgtttacg acgacgaaac ttaacgattt taactttctc gccacgaccg
    11401 tgagcaacaa cttcagcttt gattacgccg ccatcaacga aaggaacgcc gattttgact
    11461 tcttcaccgt ttgcgatcat cagcacttca gcgaactcaa cagtttcgcc agttgcgatg
    11521 tccagctttt ccaggcgaac ggtctgacct tcgcttactc ggtgttgttt accaccactt
    11581 tggaaaaccg cgtacatata aaactccgct tccgcgcaca ccttttcaat gattcagagt
    11641 gcgctataaa tattcacaat agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt
    11701 gctacagtca atacacgaag aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca
    11761 ttttttcagt acaatcaccc tatattccta accataaacc ctaagttgcc tttgttcaca
    11821 gtaaggtaat cggggcgaaa agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt
    11881 taaccgcgca agatatggcg ggtgttaatg cggcaatcct tgagcagctt aattccgacg
    11941 tccaactgat caatcagtta ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc
    12001 cgatgattgc tgtactggct gcacgagctg ttggctatga gggaaatgcg catgtcacca
    12061 ttgctgccct gatcgagttt atccacacgg cgactctgct gcacgacgac gttgtggatg
    12121 aatcagatat gcgcaggggt aaagctaccg ccaacgctgc atttggcaat gccgccagcg
    12181 tgctggtagg cgattttatt tatacccgcg cgttccagat gatgaccagc ctcggctcgc
    12241 tcaaagtgct ggaagtcatg tcagaagccg taaacgtcat cgcagaaggt gaagttctgc
    12301 aactgatgaa cgttaacgat ccggacatca ctgaagaaaa ctacatgcgc gttatctata
    12361 gcaaaaccgc gcgtctgttt gaggctgccg cgcagtgttc cgggattctg gctggctgta
    12421 cgccggagga ggagaaaggc ctgcaggatt atgggcgcta tctcggcact gctttccagt
    12481 tgatcgacga tttactcgat tacaatgccg atggcgaaca gttaggtaaa aacgtcggcg
    12541 acgatctgaa cgaaggtaaa ccgacgctgc cgctgctgca tgcaatgcat catggcacac
    12601 cagaacaggc acagatgatt cgtaccgcca tcgaacaggg taacggtcgc catcttctgg
    12661 aaccggttct ggaagcaatg aacgcttgtg gatctcttga atggacgcgt cagcgtgccg
    12721 aggaagaagc agacaaagcc atcgcagcgt tacaggtgct cccggacacc ccgtggcgag
    12781 aagcactcat cggcctcgcg cacatcgctg ttcaacgcga tcgttaatcc cctcccctca
    12841 tcccgcgcag cgcgcgggat gattccagta aattccataa aaacacttat tcagctctat
    12901 aacacctgaa aaatagggac gtacatcttt atatattctg aatattcaca ctctttacag
    12961 gaacttttta gagcaatagg ccatcaggag tatagtgatg ctcgacagaa gaagtgttct
    13021 gaatgaaagc gactaactta aggagtgagg aaagatggaa agtaatttca ttgactggca
    13081 tcccgctgac atcattgcgg gtttgcgcaa gaagggaacg tcaatggcgg cggaatctcg
    13141 cagaaatggt ttgagttcct caacgctggc gaacgcatta tcgcgcccct ggccgaaagg
    13201 agagatgatt attgcgaaag ccctgggaac tgacccctgg attatctggc catctcgcta
    13261 ccatgatccg cagacccatg agtttatcga cagaacgcag ttgatgcgta gctacactaa
    13321 accgaaaaaa tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc tctcagacaa
    13381 ttactcgcct ttcacacgct caatatttgc acctaaagcg cgcagtttgt cttcaatgcg
    13441 ttcgtagcca cgatcgatgt gataaatacg atcaactacc gtcgtccctt ccgcaataca
    13501 gccagccagc accaggcttg ctgatgcacg cagatcggtt gccataacct gtgcgccaga
    13561 aagtttttca acaccgtgac aaataacggt attgctttcg atttcggcgt gcgcgcccat
    13621 acggctcagc tctggcacat gcataaagcg gttttcaaag accgtttcgg tgatgaagcc
    13681 ggtcccttct gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg tcgggaatgc
    13741 cggatgcggc gcggtacgta cgttaacagc cttcggacgt ttgccatgca tatccaggct
    13801 aatccagtct tcgccgactt cgatgtctgc tccagcgtca cgcagtttcg ccagcacggc
    13861 gtcgagagta tctggctgcg cgttacggca gataattttg ccgcgagaga tcgccgccgc
    13921 caccaggaaa gtaccggttt cgatacgatc cggcagcacg cgatagacac cgccgcctaa
    13981 acgttccaca ccttcgatga cgatacgatc ggtgccctga ccgctaattt tcgcacccag
    14041 cgtaatcagg aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt tttcaataat
    14101 cgtggtgcct tccgccaggg ttgcagcaca catgatggtc accgttgcgc caacgctgac
    14161 tttatccatc acgatatgtg cgcctttcag acgaccatcg acggaagctt taacgtaacc
    14221 ttcttccagt ttgatggtcg cgcctaattg ttcgagacca gaaatgtgta gatcaaccgg
    14281 acgtgcacca atcgtacaac cgccaggcag tgaaacttgc ccctgaccaa agcgcgctac
    14341 cagcggcccc agcgcccaga tagaagcacg catggtttta accagatcgt aaggtgcgca
    14401 gaatacatta acgtcgcggg catcaatatg cacagaacca ttacgttcta ctttcgcacc
    14461 cagctggctt agcagcttca ttgatgtatc gacgtctttt agtttcggga cgttctggat
    14521 ctctaccggt tcttccgcca gtagtgcggc aaaaaggata ggcagagcag catttttagc
    14581 gccggaaatt gtgacttcgc cctggagctt cgttggcccc tgaacacgaa atttatccat
    14641 ttagtttgtt ctcagttaac aattcatatc cgctaccggc gaatcgccca tagctcaaaa
    14701 gccgttcagt ttgcgatcgc gcgcccactc cgcaggggta tacgctttga tcgacacagc
    14761 atgaatgcgg ttatccgcaa tatattccat cagcgggcca tagaccgtct gctgtttttt
    14821 aacccgactc atgccgtcaa acaactcacc cacggcaata acctgaaagt ggctgccatc
    14881 gccggaaacg tggacttcct ggagggagag agcgttcatc aacacgctct gaatttcatt
    14941 attttccatg ggatcttcaa tcatcagtta ataaaccagc gaaacatctt agagcaaagt
    15001 tgcgctggca taaataagca aaaagcctcg ctgataaatc agacaaggct cgacttgcag
    15061 gcaggtttgc cggacaggcg gttaacgcca tatccggcct gaaaaaattt aacgaggcag
    15121 aacatcagca ggcaaattat acaatttcgc cagggtatac actttgtcgt ttaccccctg
    15181 aagcgtcaca ttgttgccct gctttttcgc cagatcgata agatggagca gcagcgccag
    15241 tccacctgta tcaacgcggg atacacggct aagatcgata caggtaatcc ccttcaccgc
    15301 ttcctcacgc atttcccaaa gcggtagcag aacgtcctga tccagctctc cggataacgc
    15361 cagcgtgtca cccgtctgca tccagctcag tgactcgctc attatttttt ctcttccaga
    15421 gtgattttct gttgagaaat cgatttcagt tgcgcagtca ggccgtcaat acctttggta
    15481 cgcagcagcg ttccccactc gttttgtttg gtggtgatca tactgacgcc ttcagcaatc
    15541 atgtcgtaag cctgccagtt gcccgtctgg gagtttttac gccactggaa gtccagacgc
    15601 accggcgggc ggccattcgg gtcaataatg gtaacgcgaa taggcacaat ggttttatca
    15661 cccagcggct gttctggcgc aatctgatag gtttgaccgt gatacatcgc cagcgcctga
    15721 ccgtaagcct gcttcaggta ctcacggaaa gcggcaaagt aagcatcacg ttgagctggg
    15781 gtcgcactct tgtaatactg acccagcacc agcgcaccgg cgtatttcac ctgtacgtat
    15841 ggcagcagtt cctgatcaac aatggtacgc agataatccg ggttggcccg aatttgtggt
    15901 tgctcattct tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag cttatacgga
    15961 ttggtctggt ctgccgcggt tgccgcactc agcggtgcaa tcaccagcaa agcgaccatc
    16021 attaaacgtt taaacatgcg tcggttctcc tgaaattatt tcgttgtacc cacaggttca
    16081 gtggtttcat tattacctgg cgcagcagct ggcgcatcgc cactattctt attgtcatcg
    16141 cctttactac cgtaaaggaa ctgaccaata agatcttcca gcaccatcgc agacttggtg
    16201 tcctgaattg tatcgccatc cttcaggata gcagtcccca gttccgggtc ttcaaaaccg
    16261 acgtttaatg ccagatattg ttcccccagc aggccggaag tacgaatgct cagcgaactg
    16321 gtatcaggaa tgtggttata acgttgttca atttccagcg ttacgcgcgg cagataggtt
    16381 ttcgggtcca gcgtaatatc cgccacccgg cccacaacaa caccaccaat gctgaccgga
    16441 gagcgcgctt tcaggccgcc aatgttatcg aacgtcgcat aaagcgtgta ggtcggttca
    16501 gtacgtatgg acgtcacgtt cgccgccttc aggcaaacaa acagcgccgc cagtagtgct
    16561 gctaataaaa agatacccac ccaaatttca ttttttttcg tttgcatgaa ctcaattccc
    16621 aaacatcaat gcggtcagca caaaatccag ccccagaaca gccagagacg agtggacaac
    16681 ggtgcgagtg gttgcccggc taatcccggc agacgtcggg atggcgtcgt agccgttaaa
    16741 caacgaaatc cacgtcaccg tgatggcaaa caccacgctc ttaatcagac agttgaccag
    16801 atccatacgc cagtcgacgg cattttgcat tgccgaccag aagaacccgc tatcaatgcc
    16861 tttccaactg acgccgacca gagatccgcc ccagatcccc acggcgacga aaataaccgt
    16921 caacagtggt aatgaaataa ccccagccca gaaacgggga gaaataaccc gacgcagcgg
    16981 atccaccgcc atcatctcca tactggagag ttgctctgta gcgcgcatca ggccgatttc
    17041 tgcggttagc gccgaaccag cacgcccggc aaacaacaac gcggcaacca ccggccccag
    17101 ttcacgcagt agcgataacg ccaccagcat acccagactg gtttccgcac tataagtggt
    17161 cagaaccaga taaccttgca gccccaacac cattccgatg aacacgccag aaaccacaat
    17221 aatcagcatc gacaggacgc cgacattata gagctggcgc accagcagcg gcgcatgttt
    17281 gcgaaattcc ggtttgccga ccagcgcatt gaataacatt aacccggccc gcccgaacgt
    17341 tctcagggtt ttaatccctt tatgtccgag cgacgccagc gcatttaaca gcatgagtgg
    17401 cttaactccc tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg aacggaacag
    17461 gcccgtcagc tatcccgtcc agaaactgac gtacgcgtgg atcaggattc gcctgcaacg
    17521 cctgggcact gccatgagcg acaatttttt tgtccgccag gatccaggcg tgatcggcaa
    17581 tacttaacac ttccggcaca tcgtgagaaa ccaccacaca agtcacaccc agcgcgctgt
    17641 tcagctcaga aatcagcttc accagtacgc ccatggtgat gggatcttgc ccaacaaagg
    17701 gttcatcaaa catgatgaga tccggctcca gcgcaatcgc acgcgccagc gctgcacgcc
    17761 gcgccatccc accggaaagt tcagaaggca ttagtttagc cgctccacgc agccccacgg
    17821 cctccagctt catcatcacc gtactatgca acaatggcgc gggaagttgg gtatgttcgc
    17881 gcagtggata ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc cctgactgaa
    17941 ataacatgct catccgtttg cgcactgtat acaggcgcga acgagacatc gccggaatat
    18001 tctcaccatc gaaaagaatc tcaccatgat ctggtgcgat ttgcccgcca atcagacgga
    18061 gtagcgtcgt tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc cctcgcggca
    18121 cggtcaggga aatattatcg aagatgcagc gattgccacg cgtaaaactg acatcgcgca
    18181 tatcgactaa attcgccaca gactgctcca taattcaccc ttcgtcttgc gttgattttc
    18241 taagcatggc gctcaattta accttgaacc caacatattt acagaatatt acccgccgtg
    18301 gttagcgaaa gctggcattt gttttacttt ttagccgcat aaagtcaaaa ttaagaatcc
    18361 gttacggctt tctgaaaatc ttcagcggac cggcgagtat acctgaagaa aggacgttag
    18421 atgcttttag ctacggcact gttaattgtt ggtttacttt tggtcgttta cagtgccgac
    18481 cgcctggttt ttgccgcgtc tattctttgc cgaacctttg gcatcccgcc gctaatcatc
    18541 ggcatgacgg tggtcagtat tggtacatcg ttaccagaaa tcatcgtctc gcttgccgcg
    18601 tctctgcacg aacaacgcga tttagccgtt ggtacagccc tcggctcaaa cattatcaat
    18661 atattgctga tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca ttctgatgtt
    18721 ctacgccgtg aattaccctt aatgttgttg gtcagcgtgg tggccggttc cgtactctat
    18781 gacggacaac ttagtcgaag cgatggtatc tttctcctct ttctggctgt gctatggctg
    18841 ctgttcattg ttaaacttgc acgtcaggct gaacgtcagg ggactgacag cctgaccaga
    18901 gagcagcttg cagagctgcc tcgtgacggc ggattgcccg tcgcgttttt atggctcggc
    18961 attgcgctta tcatcatgcc agtggccacg cggatggtgg ttgataacgc cacggtgctg
    19021 gcgaattact ttgccatcag cgagttgacg atgggtctga cggcaattgc tatcggaacc
    19081 agcctgccgg aactggcaac cgcaatagcg ggggttcgca aaggtgaaaa cgacattgct
    19141 gtcggaaata tcattggcgc aaacattttt aatattgtca tcgtattggg tttacccgcg
    19201 ctgataacgc caggagagat tgatccactg gcgtacagtc gtgactacag cgtgatgttg
    19261 ctggtgagca ttatttttgc gttgctgtgc tggcggcgct ccccgcaacc gggccgtggt
    19321 gtaggggtat tattaactgg cggatttatc gtatggctgg cgatgttgta ctggttatcg
    19381 ccaatactcg ttgaataact ggaaacgcat tatgtcgcac gtagagttac aaccgggttt
    19441 tgactttcag caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc tggcggagct
    19501 tgatcaatac atcaatcaga atttcacgct tgcctgtgaa aagatgttct ggtgtaaagg
    19561 gaaagttgtc gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa tggccgcaac
    19621 gtttgccagc accggtacac cttcattttt cgtccatcct ggtgaagccg cgcatggtga
    19681 tttaggcatg gtcaccccac aggatgtggt gattgctatc tctaactcag gtgaatccag
    19741 cgaaatcacg gccttaattc cagtgcttaa gcgtcttcac gtaccgttaa tctgcatcac
    19801 cggtcgcccg gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg ttaaagtagc
    19861 gaaagaagcc tgtccgttag ggctggcacc gaccagcagc accaccgcca cgctggttat
    19921 gggcgatgcc ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg aagattttgc
    19981 gctctcacac ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa acgatattat
    20041 gcatacgggc gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg acgcattgct
    20101 ggaagttacc cgcaaaaatc ttggtgtgac tgtcatttgc gatgacaata tgatgattga
    20161 aggcatcttt accgacggtg atttacgccg tgtcttcgat atgggcgtgg atgttcgtca
    20221 gttaagtatt gccgatgtga tgacgccggg gggaatacgt gtgcgccctg gcattctggc
    20281 cgttgaggca ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg ttgccgatgg
    20341 cgaccattta ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg tagtgtaaag
    20401 attcaaggat aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg ttacggccct
    20461 gtcagcgccg acgttatagc aaaagcagag aacattcgtc tgctgatcct cgatgtcgat
    20521 ggcgtactgt cagatggcct gatttatatg ggcaataatg gcgaagagct gaaagcgttc
    20581 aatgttcgtg acggttatgg cattcgttgt gcgctcacct ctgatattga agtcgctatc
    20641 attaccgggc gaaaggctaa actggtagaa gatcgttgtg ccacattggg gatcactcac
    20701 ttgtatcagg ggcagtcaaa caaactgatc gcctttagcg atttgctgga aaaactggcg
    20761 attgccccgg aaaatgtggc ttatgtcggc gatgatctca tcgactggcc ggtaatggaa
    20821 aaagtgggtt taagcatcgc cgtggccgat gcgcatccac tgttaatccc gcgcgccgat
    20881 tacgtgacgc gcattgctgg cggtcgtggt gcagtgcgtg aagtttgcga cttattactc
    20941 ctggcgcagg gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa gccagacgtt
    21001 gggttatcat tgtgctgtca ctggcggttc tggtgatgat cggcattaat atggccgaaa
    21061 aagacgatac cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa agcgagcata
    21121 cggacacgct cgtctataac ccagaagggg cactaagcta tcgattgatt gctcaacacg
    21181 ttgaatatta ttccgatcag gccgtttcgt ggtttacgca gccggtactt accacgtttg
    21241 ataaggataa aatcccgaca tggtccgtaa aagcagataa agccaagctg accaatgacc
    21301 ggatgctcta tttatatgga cacgttgaag tcaacgcact cgtgccggac tctcaacttc
    21361 gcagaatcac gacggataac gcgcagatca atctggtgac gcaggatgtt acctctgaag
    21421 acctcgtcac gttatacgga acaacattta actccagcgg tctgaaaatg cgcggcaact
    21481 tacgcagcaa gaacgccgag ctgattgaaa aggttagaac atcctatgaa attcaaaaca
    21541 aacaaactca gccttaatct tgtgcttgcc agctcacttc tggccgccag cattccggca
    21601 tttgccgtaa ccggagacac tgatcagccg atccacattg aatcggacca gcaatctctt
    21661 gatatgcaag gcaacgtggt tacctttacc ggcaatgtca tcgtcaccca gggcaccatc
    21721 aaaattaatg ccgacaaagt ggtcgttacc cgtccgggcg gcgaacaagg taaagaagtg
    21781 attgacggct acggtaaacc ggcaacgttc taccagatgc aggacaacgg taaacccgtt
    21841 gaaggtcacg cttcacagat gcactacgaa ctggcaaaag attttgtcgt tctgacgggt
    21901 aatgcttatc tgcagcaggt cgatagcaac attaagggcg ataagatcac ttacctggtg
    21961 aaagagcaga aaatgcaggc tttcagcgac aaaggcaagc gcgtaaccac cgttctggtg
    22021 ccgtcgcagc tgcaggacaa aaacaataaa ggccagaccc cggcacagaa gaagggtaat
    22081 taattcgtta tggcaacatt aactgcaaag aaccttgcaa aagcctataa aggccgtcgc
    22141 gtggtagaag acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct gctggggcca
    22201 aacggtgccg gtaagaccac cactttctac atggttgttg gcattgtgcc gcgcgatgcg
    22261 ggcaacatca ttattgatga tgacgatatc agtctgctgc ctctgcatgc acgcgcgcgc
    22321 cgcggtatcg gctatctgcc gcaggaagcc tccattttcc gtcgcctcag cgtttacgat
    22381 aacctgatgg cggtactgca aattcgtgac gacttgtctg ctgaacaacg tgaagaccgc
    22441 gcgaacgagc tgatggaaga gtttcacatt gagcacctgc gtgacagcat ggggcagtca
    22501 ctctccgggg gtgaacgtcg ccgtgtagaa attgcccgtg cactggctgc gaatccgaaa
    22561 tttattctgc tcgacgaacc gtttgccggg gttgacccga tctcggttat cgacattaaa
    22621 cgcatcattg agcacctgcg cgacagcggc ctgggcgtac tgatcactga ccacaacgtg
    22681 cgtgaaacac tggcggtttg tgaacgcgct tatatcgtca gtcaggggca tttgatcgcc
    22741 cacggcacgc ctacagaaat cttacaagac gaacacgtta agcgtgtata ccttggggaa
    22801 gacttcagac tctgataggg tagaagtttg cgatgtttta gcaggagagt acgattctga
    22861 acatgaagca aggtttgcaa ctcaggctta gccaacaact ggcgatgacg ccacagctcc
    22921 aacaggcaat tcgtctgttg cagttgtcga cgctggaact tcagcaggag ctacagcagg
    22981 cgctggagag taatccgctg cttgagcaaa tcgacactca tgaagaaatc gacacccgcg
    23041 aaacgcaaga cagtgaaaca ctggacaccg ccgacgcgct cgaacaaaaa gagatgccgg
    23101 aagagctgcc gctcgatgcc agttgggaca ccatttacac cgctggtaca ccatccggca
    23161 ccagcggtga ctacattgac gacgagctgc cggtctatca gggcgaaacg acgcagacct
    23221 tgcaggatta cctgatgtgg caggtcgagc tgacaccgtt ttccgacact gaccgcgcta
    23281 ttgctacctc tatcgtcgat gccgttgatg acaccggtta tctgactgtc ccgctggaag
    23341 atattctcga aagtatgggc gatgaagaga ttgacatcga cgaagttgaa gccgtcctta
    23401 agcggatcca acggtttgat ccggtcggtg tggcggcaaa agatctgcgt gactgtctgc
    23461 tgatccaact ctcccaattc gataaaacca cgccatggct ggaagaggcc agactgatca
    23521 ttagcgatca tctcgatctg ttagccaatc acgacttccg cactttaatg cgcgtcacgc
    23581 ggctgaaaga agatgtgctg aaagaagccg tcaatctgat ccagtcgctc gatccgcgcc
    23641 ccgggcagtc gatccagacg ggcgaacctg agtatgtcat tccagatgtg ctggtgcgta
    23701 agcataacgg tcactggacg gtagaactca acagtgacag cattccgcgt ctgcaaatca
    23761 accagcacta cgcctcgatg tgcaataacg cgcgcaacga tggtgacagc cagtttatcc
    23821 gcagcaatct gcaggatgcc aaatggttga tcaagagcct ggaaagccgt aacgatacgc
    23881 tactgcgcgt gagtcgctgt atcgttgaac agcagcaagc cttctttgag caaggcgaag
    23941 aatatatgaa accgatggta ctggccgata tcgcccaggc tgtcgaaatg catgaatcga
    24001 cgatatctcg cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt tttgaactga
    24061 agtatttctt ttccagtcac gtcaataccg agggcggcgg cgaagcttcc tccacggcga
    24121 ttcgtgcgct ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg ttgagcgaca
    24181 gcaagttaac ctctttgctg tcggaacaag gtatcatggt ggcacgccgc actgttgcga
    24241 agtaccgaga gtctttatcc attccgccgt caaaccagcg taaacagctc gtttgaccca
    24301 accgataagg aagacactat gcagctcaac attaccggaa ataacgtcga gatcaccgag
    24361 gcactgcgcg aatttgttac agccaaattt gccaaacttg agcaatattt tgaccgaatc
    24421 aaccaggtct atgttgttct gaaagtggag aaagtcaccc acacctcaga tgcaacactg
    24481 catgtaaacg gtggtgaaat tcatgccagc gcggaaggtc aggacatgta cgctgccatt
    24541 gatggcttaa ttgataagct ggcacgccag ttgaccaaac ataaagataa actgaaacaa
    24601 cactaattgt ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca caacgggcca
    24661 tttgtacggt taatgctccg agcctgttcc actgtttgag tgggcaggtt cttaggtgaa
    24721 attatgacaa ataatgatac aactctacag cttagcagtg ttcttaacag ggaatgtacg
    24781 cgaagccgcg tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag cgagctggcg
    24841 gcgaaacaac ttagtctgcc gcctcaggtg gtttttgaag ctatcctgac gcgggaaaaa
    24901 atgggcagta ccggtatcgg caatggtatt gccattccgc atggcaaact ggaagaagat
    24961 actctgcgcg ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt cgatgccatt
    25021 gacaaccaac cggtagattt actatttgcg ttgctggttc cggctgacca aaccaaaacg
    25081 cacctgcata ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat ctgccgccgt
    25141 ttacgcgcag cccagagcga tgaagagctg tatcaaatca ttacggatac cgaaggtact
    25201 ccggatgaag cgtagttatt cggtaatgtc tcttttagac gttgtgagga gaaacagtac
    25261 atggtactga tgatcgtcag cggacgttca ggttcaggta aatctgtcgc cctgcgtgcg
    25321 ctggaagata tgggttttta ctgcgtggat aaccttcccg tagtgttgtt acccgatctg
    25381 gctcgaaccc tggccgatcg cgagatttct gccgccgtca gcattgatgt tcgtaatatg
    25441 ccggagtcac cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc tttctcaccg
    25501 caactcctgt tcctggatgc cgaccgtaat accttaattc gtcgttacag tgacacgcgc
    25561 cgactgcatc cgctttccag caaaaacctg tcgctggaaa gtgctatcga caaagaaagc
    25621 gatttgctgg agcctctgcg ttcgcgagcg gatctgattg tcgatacctc agaaatgtcc
    25681 gttcacgagc tggcagaaat gctgcgtacc cgtctgctgg gtaaacgtga acgtgaactg
    25741 accatggtct ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc agattacgtc
    25801 tttgacgtgc gcttcttgcc gaacccgcac tgggatccga aactgcgtcc aatgacaggt
    25861 cttgataaac ctgtcgccgc gttcctcgac cgccacacag aagtacacaa ttttatctac
    25921 cagacgcgaa gctatcttga gctatggtta cctatgctgg aaaccaacaa ccgtagctac
    25981 ctgacggtcg ccattggttg taccggcggg aagcaccgtt cggtgtatat tgcagagcaa
    26041 ctggcagact acttccgctc gcgcggtaaa aacgtccagt cacgccatcg tacgctggaa
    26101 aaacgtaaac catgaccgtc aagcaaactg ttgaaatcac aaacaagctg ggcatgcatg
    26161 cccggcctgc aatgaagctg tttgaattaa tgcaaggttt tgacgcagaa gtgctgttac
    26221 gtaatgacga aggtaccgag gccgaagcca acagcgttat tgcactgctg atgctggatt
    26281 ctgccaaagg acggcagatt gaagttgaag cgaccggtcc acaggaagag gaagcactgg
    26341 ccgccgttat cgccctcttt aattctggtt ttgatgaaga ttaatcttca tcactttgac
    26401 attcaaatcc cttcaagctc ccccgacaat aagaaaagcg cgtgctgaaa tcgttctcaa
    26461 tcaacgtcat ttgtacattt tgtgcgcttt tcactttcag aagaacctta agaaaacctt
    26521 aagaagcatt gtttaggttt cgtttaagtt aatcgaccat actggagatc gtcagaaaat
    26581 atttccagga gatggcatga ttcgcttatc tgaacaaagt cccctgggca ccggaagaca
    26641 tcggaaatgt tatgcgcatc cggaagatgc ccaacgctgt atcaagattg tctaccatcg
    26701 tggcgatggt ggcgataaag agatccgccg tgagttaaag tactacgcgc atcttggtcg
    26761 ccggttaaaa gactggagtg gaataccgcg ctatcacggt accgttgaga cggattgcgg
    26821 aaccggttat gtctacgatg tgattgcaga ttttgacggc aaaccctcca ttacgctgac
    26881 cgaatttgct gaacaatgcc gttacgaaga agacattgca caactgcgcc agctactgaa
    26941 acagctgaag cgttatttgc aggataaccg tatcgtgacg atgtcgctga agccgcagaa
    27001 tatcctctgt caccgcatca gtgagtcaga ggttatcccc gtcgtctgcg acaacattgg
    27061 tgaaagcaca ttgattccac tggcgacctg gtcgaagtgg tgttgtctgc gtaagcaaga
    27121 gcggttatgg aaacgattta tcgcccaacc ggcgctggct attgccctgc agaaagatct
    27181 gcaacctcgt gagagcaaaa cgctggccct cacttcgcgc gaagcttaat ccagctggtg
    27241 ctgttgcata aacgactcac cgcctaactg atacatctgc cgtaaaatcc acgcctgacg
    27301 gctacgcacg tagcccgatg gcgcggagac tttgaaacga agtggattag gtaatacagc
    27361 agccagtaat gcagcttccg agcgggtaag tttgctcgcg ggtttgtgga aataacgttg
    27421 tgccgcagct tcgacgccaa acacaccgtc gccaaattcg gcgatattca ggtaaaccgt
    27481 caggatacgc tttttgctcc agactgtttc tatccccagc gttaatccag cttccagccc
    27541 ttttcgtacc cagctacgcc catcccataa aaagagattt ttggccgtct gctgagaaat
    27601 cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg tgcgccaggg ctttctcaat
    27661 ggaagcgaca tcaaagcccc agtgctcagg aaatttctga tcttctgcgg caatcaccgc
    27721 cagtcccatc cacggcgaga tttgatccat actgacccag tcagaatgtg ccacgtaacg
    27781 aaaattgcca tgcagccagg cgctgacctg tcgctcgacc attaccgctg agaagggaac
    27841 aggcgcaaca ctaaacaacg cgatgccccc gccccagaaa acggcgagga caaccattaa
    27901 ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag cggcttttac tcattcagcc
    27961 agaaccagca cgcgggaaac cagcttatca atgccgctcg ccgcttctgc aatgttctgc
    28021 gccagcatat atgctggggt ggtgacaatc ttattgtctt catcaaccac gatatcatcg
    28081 acaggacatg gcacatgctc cgcgcccatc tcttccagca cttctgcggt atcgatatca
    28141 gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg gcagcatcgc cggggcaata
    28201 cacataaaac caagcggttt tccggcctga tgcatcgctt gtgccagcgc ctttaattca
    28261 cggtcaacgg tgcattcgct accaagactg gcaaaattgc ttaaattctt cgccgcgcca
    28321 aaaccccccg gcacaatcaa cgcatccagt tcagcggcat cggcctgggc cagaggacgg
    28381 atttcaccgc gcgttattcg tgccgcttca atcagcacat tgcgcgtttc cgtcatcgct
    28441 tcgcccgtta aatggttgat aacatcaacc tgctgcttat ccggtgcaaa gcagaccgcc
    28501 tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg cttcatgaat ttcagaaccg
    28561 tcatagacgc cgcatccgct cagaattacg ccaattttct tcattgtgat catcctttcg
    28621 caattgactg aaacacatta atttttttaa taaaaatggt acgcatcaca catttaactg
    28681 attcatgtaa caaatcattt aagttttgct atcttaactg cgtgcggcct gaaaaacagt
    28741 gctgtgccct tgtaactcat cataataatt tacggcgcag ccaagatttc cctggtgttg
    28801 gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt ttagtggctt ttgccaccca
    28861 cgctttcagc acttctacgt cgtgacgcca ctcttctttc atctcttcaa tccattcacc
    28921 gacgttatct tcccaggcag gaaggtcagg agactgaatt tgctgaccca gctgttgcag
    28981 atggcgtaac cccactgacc ccgccgcacc tttaatttta tgtccttcct caacaatgcc
    29041 ttttttatcc tgcgccgtca gattcgactc cagcacgctg acatagcccg gcatcatctt
    29101 ctcaaacacc gctaacccgt cggtgatcag cttcggtcct acaagtgcga gatactgttc
    29161 cagcatggga atatcgagca atgcttctga tttactgttc tcttctgtcg tcaccgtact
    29221 ctcctcatca tcctgggtat cccagaattt cttgatcatc gcggttagcg ccggaaccga
    29281 aagcggctta ctcagcacat catccattcc agcattgagg tactcttgtt tgtctttcag
    29341 cacgttagcg gttaaggcca ccagcggcgg taaatcctcg cgcggataac gcttcgtcag
    29401 ttcacgagag atatccagcc cggtcatatc tggcaactga atatccagca acaccaggtc
    29461 gtattcgccc ggtttaaaca tctccagcgc cgccttgccg gtcatggcga catcaacgct
    29521 gttacctaat ttttccagca cagaacgcgc aacaatcacg ttcagttcaa tgtcttccac
    29581 cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca tcaaacgcat catcgacctc
    29641 ttctgctacc gacggtgcgt ggatcgtcaa cgtaaaggtt gaacctttgc cctgttcgct
    29701 ggtaaccgta atatcgccgc ccatattttt cgccagacga cgagaaacgg ccagaccaat
    29761 accggtgccg gtggcaggtt taccgccatg actgtctttc acctggtaat acatggcgaa
    29821 aattttatcc agctcatcct gcggaatgcc gataccagag tcttccactt caaaatgcag
    29881 catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg ccttgctggg tgaatttgac
    29941 ggcgttactg atgagattcc acaggatctg ccgtaaacgc gtcccgtcgg taatgacctg
    30001 atgcggtaat ggcagcgtag gctccaggtt aaagcgcaat cctttttgtt gcgcctgcaa
    30061 tgcggaaaga ttttccagat cggcaaggaa gctggtgaaa tcaaccggct gattatcaag
    30121 ctggacctta cgccgttcca tcttatccat gtcgataata tcgttgaaga tattcccgag
    30181 cgtgacggcc gaaacatgga tggttttgag atatttttcc tgctcggcgg tgagttcggt
    30241 atccagcaga atgcggctca ggccgacgat accattcagc ggtgtacgca attcgtgact
    30301 gatggtggag ataaacgtcg ttttgtcgcg gctggcccgt tcaagcgcat cctgataccg
    30361 cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg tgacgtttac ccacgcggtc
    30421 gtagtacggc actttacgga tttcaaagca ggctttgcgt ccgtcgggat aatccagcca
    30481 ctgctcatag gtcagtgaca cattatgacg gaacactttt tcatcggttt caatgacttt
    30541 cgcagcggct tccggtgagt aaacatcagc aggtttcaga tgaaccagtt gtttttcgct
    30601 ttttccggtc agcagctcca tcgcgcggtt acagccggaa aactctttat cttcgttacg
    30661 ataaaaaacc aggtcgggtg aagcgtcaag gaaggaacgt aagaatgagg attgctgctc
    30721 gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt ttcagttggc cgaaggtttc
    30781 ctgcagttct gcttccgctt tttcacgaac ggcgatttcc tgatttagct gggcaatatt
    30841 atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc atctcctcca gtttttgcac
    30901 cagtcgtgac agacgttgtc gtgactcctc cagttgctcg acgaccaccg atagaaagta
    30961 gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa cgaataacat caatgctttc
    31021 gacctgacca tgcagcacca tggttaccgc catttgtacc acaatggcaa gaacgacgag
    31081 ggccagcgcc agtaacattg agaagcgcac cagacctaac ttcatcatca ggtcaacata
    31141 atactgcgcc agcagacgaa tttgcttcat tagggaattc cttcacgaca acctgcccca
    31201 ataataccca attctgacag ttacgttata aattgtgtga gaaatgcgga attcccctca
    31261 atggcctacg ccgttggagg tagccacgga cgtcccagcg ccgatccctg tacgccatgt
    31321 tcattcagat aacgatccag ctcgaccatt cccgtccatc gattttcgca ccacagcgga
    31381 gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt ggtagatcac ttccggtggc
    31441 gtatggcgaa tcatttctcc ggcagtgagc gtgtaatcct ccagttcaat tccgttcaaa
    31501 cgccccgctt cccaggcttt cgccataata ctgcctttca caatatgcag cggatgcagc
    31561 tttatgccgt ccacgccggt ttcaaccact cgctccagcg tttgcaaaca ttccgtctgc
    31621 ccttcgccag gcaggccgac aattaagtgg gaacacacct tcagcccgcg ctgacgtgcc
    31681 agctgggttg tacgctgata acaggcaaaa tcatggccgc ggttgatgcg atgcagcgtt
    31741 ttgtcgtggg cggtttgtag ccccagctcc agccacactt cgtaaccctg gtccttatat
    31801 tcgcaaagca gatccagcac cgcatccggc acgcagtccg ggcgggtacc aacacacaaa
    31861 ccgacaatat tggcctggct caccgcctgc tgatacatag aacgcagcac ctgaacttcc
    31921 gcaaaggtgc tggtatacgc ctgaaagtag gccagatagc gtttagcgcg gttaactaaa
    31981 ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct gctgcgcttc atcggcaaac
    32041 gaggccacat tacagaatgt gcagccacca cgcccgatgg taccgtcacg gttagggcag
    32101 ctaaaaccgc cgtgcagcgt cagcttatgc accttttgcc cataacgacg ggtgagatca
    32161 ccaccaaaca tattgactaa tttctgtaac tgcataatct gatagaccgc gccttgaaaa
    32221 gaggccaaag cctgccattt ttagcccaat tcggcgatga cctggatcaa tcgtcccggc
    32281 ccgcttttat caactgcata atcaatcaaa attaccgaaa tttcatgcac aatcacataa
    32341 atcacttttg cttatcttgt gtcagatttt tttatctcct tatggatttt aggcaaaaac
    32401 agtagcatga aacgttatta ccaattaagg cagtataaaa tgctggtttt ctcgtcagtt
    32461 caaggcagga taagggttaa cacgccttta tgacagtcag gaattggctg tttctctaac
    32521 gacgcccctt ttagccttaa atagaaaatc cactttaatt tcagccaatt aataaagaat
    32581 tttgcgctaa agcacatttc tgtaccaata agcttgccat ttgacctgta tcagctttcc
    32641 cgataagttg gaaatccgct ggaagctttc tggatgagca gcctgctcat catatttatg
    32701 cagtaattga gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa caagggggcg
    32761 aatgcgaggc gcgcgtatga cacgcaaacc ccgtcgccac gctctttctg tgcccgtgcg
    32821 cagcggttcg gaagtggggt tcccgcagag cctgggggag gttcacgata tgttgtacga
    32881 taaatccctt gagagggata actgtggttt cggcctgatc gcccacatag aaggcgaacc
    32941 tagccacaag gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc accgtggcgc
    33001 gattctcgcc gatggtaaaa ccggtgacgg ttgcggcttg ctgttacaaa aaccggatcg
    33061 cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta gcaaaaaact acgccgtcgg
    33121 gatgctcttc ctgaataaag atcctgaact cgccgctgcc gcacgccgca tcgttgaaga
    33181 agagctgcaa cgcgaaacgt tgtcgattgt gggctggcgt gatgtcccca ctaacgaagg
    33241 cgtgctgggt gaaatcgccc tctcctctct gccacgcatt gagcaaattt ttgtgaacgc
    33301 cccggcaggc tggcgtccgc gcgatatgga gcgccgtctg tttatcgccc gccgccgcat
    33361 tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt agcctgtcga atctggtcaa
    33421 tatttataaa ggtctgtgta tgccggcgga tctgccgcgc ttctacctgg acctcgcgga
    33481 cctgcgtctg gaatcggcta tctgcctgtt tcaccagcgc ttttccacta acaccgtacc
    33541 gcgctggccg ctggcgcagc cgttccgcta cctcgcgcat aacggtgaaa tcaacaccat
    33601 caccggtaac cgccaatggg cgcgtgcgcg tacctataaa ttccagacgc cgctgatccc
    33661 tgacctgcac gacgccgcgc cgttcgtcaa cgaaaccggc tctgactcca gttcgatgga
    33721 taacatgctg gaactcctgc tggcaggcgg gatggatatc atccgtgcca tgcgtctgtt
    33781 agtgccacca gcctggcaga acaacccgga tatggacccg gaactgcgtg ccttctttga
    33841 ctttaactcc atgcatatgg agccgtggga tggtccggca gggatcgtga tgtccgatgg
    33901 ccgtttcgcc gcctgtaacc tcgaccgtaa cggtctgcgt ccggcacgct acgtcatcac
    33961 caaagataaa ctgatcacct gcgcctctga agtcggtatc tgggattatc agcctgacga
    34021 agtggtcgaa aaaggccgcg tagggccagg cgaactgatg gtgatcgaca ctcgcagtgg
    34081 gcgcattcta cactcggcag aaaccgatga tgacctgaaa agccgccatc cctataaaga
    34141 gtggatggag aaaaacgtcc gccgtctggt accgtttgaa gatctgcccg atgaagaagt
    34201 gggtagccgc gaactggacg acgacacgct tgccagctac cagaaacaat ttaactacag
    34261 cgcggaagag ctggattcgg tgatccgcgt actgggtgaa aacggtcagg aagcggtcgg
    34321 ttcgatgggc gacgataccc cattcgccgt gctctccagc cagccgcgca ttatttacga
    34381 ctacttccgc cagcagtttg ctcaggtaac caacccgcca atcgacccgc tgcgcgaagc
    34441 gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg aacgtcttct gtgaagcaga
    34501 aggccaggcg caccgtttaa gctttaaatc gccgattctg ctctattccg atttcaaaca
    34561 gctcacgacg atgaaagagg atcactaccg cgcagatacg ctggatatca cctttgacgt
    34621 cactaaaacc acgctcgaag cgacggtgaa agagctgtgc gacaaagccg aaaaaatggt
    34681 acgtagtggc accgtgctgc tggtgctctc cgaccggaat atcgctaaag atcgcctgcc
    34741 ggttccggct ccgatggcgg ttggcgcgat ccagacccgt cttgtcgagc aaagcctgcg
    34801 ttgcgatgcc aacatcatcg tagaaaccgc cagcgcccgt gatccgcacc acttcgccgt
    34861 gttgctgggt ttcggcgcga cggctattta tccatacctt gcctatgaaa cgctgggccg
    34921 cctggtagac acccacgcga ttgccaaaga ttatcgtacc gtgatgctca actatcgtaa
    34981 cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca ccatcgcctc
    35041 ttaccgctgc tcgaaactgt ttgaagcggt cggtctgcac gatgatgtcg tgggcctgtg
    35101 cttccagggg gcggttagcc gcattggggg ggctagcttt gaagacttcc agcaggatct
    35161 gctgaatctg tcgaaacgag cctggctggc gcgtaagcct atcagccagg gcggcctgct
    35221 gaaatacgtg cacggtggcg aataccacgc ctacaatccg gacgtggtgc gcacgctgca
    35281 acaggcggta cagagcggcg agtatagcga ctatcaggaa tacgcgaagc tggttaatga
    35341 gcgtccggca accacgctac gcgatctgct ggcaattacg ccgggtgaaa acgcggtcaa
    35401 cattgctgat gttgaaccgg caagcgaact gtttaaacgc ttcgataccg ccgcgatgtc
    35461 tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg gaagcgatga acagcatcgg
    35521 cggtaactca aactccggcg aaggcggtga agacccggcg cgctatggca ctaacaaagt
    35581 gtcgcgcatc aagcaggtgg cgtccggtcg cttcggcgtt actccggcgt acctggtcaa
    35641 tgccgacgtc attcagataa aagtcgccca gggcgcgaag ccgggtgaag gaggtcagtt
    35701 accgggtgat aaagtcactc cttacattgc caaactgcgc tattcggtgc cgggcgtgac
    35761 gctgatctcc ccgccgccgc accacgatat ctactctatt gaggatttag cgcagctgat
    35821 tttcgacctc aagcaggtta acccgaaagc gatgatctcc gtgaagctgg tttccgaacc
    35881 gggcgtaggc accatcgcga ctggcgtggc aaaagcttat gccgatttaa tcaccatcgc
    35941 aggctatgac ggcggcaccg gcgcaagtcc gctttcatcg gtgaaatacg caggctgtcc
    36001 gtgggagctg gggcttgttg aaacccaaca ggcgctggtt gccaacggct tgcgtcacaa
    36061 gatccgtttg caggtcgatg gcggcctgaa aacgggtgtc gatatcatca aagcagcgat
    36121 tcttggcgcg gaaagttttg gcttcggcac tggcccgatg gtagcgcttg gctgtaaata
    36181 tctgcgaatt tgccacctga acaactgcgc cacgggtgta gcaactcagg atgacaaact
    36241 gcgtaagaat cactatcacg gcctgccgtt caaagtgact aattactttg agtttatcgc
    36301 ccgtgaaacc cgcgagctga tggcacagct gggggtaacg cgtctggtgg atctgattgg
    36361 tcgcaccgac ctgctgaaag agctggacgg tttcaccgcc aaacagcaga aactggcact
    36421 gtcgaagctg ctggagactg ctgagccgca tgctggtaaa gcgctctact gcaccgaaaa
    36481 caacccgccg tttgataacg gcctgctgaa cgcgcagttg ctgcaacagg caaaaccgtt
    36541 tgtcgatgaa cgccagagca aaaccttctg gttcgatatc cgcaacaccg accgttctgt
    36601 cggcgcatcg ctttcaggct atatcgccca gacgcacggc gatcaggggc tggctgccga
    36661 tcctatcaaa gcgtacttca acggcaccgc aggccagagc ttcggcgtgt ggaacgcggg
    36721 cggcgtggaa ctgtacctga ccggtgatgc caacgactat gtcggtaaag gcatggcggg
    36781 cggcttaatc gccattcgtc ctccggttgg ctcagccttc cgcagccatg aagcgagcat
    36841 tatcggcaac acctgcctgt atggcgcgac cggtggtcgt ctgtatgccg caggccgcgc
    36901 gggtgaacgt ttcggcgtgc gtaactccgg tgctatcacc gtggtagaag gcattggcga
    36961 caacggctgt gaatatatga cgggtggtat cgtctgtatt ctgggtaaaa ccggcgttaa
    37021 cttcggcgca ggcatgactg gcggcttcgc ttacgttctc gatgaaagcg gcgatttccg
    37081 caaacgcgtt aacccggaac tggtcgaggt cttaagcgtt gacgatctgg cgatccatga
    37141 agagcatctg cgcggtctta tcaccgagca tgttcagcat accggttctc agcgcggtga
    37201 agagattctg gcgaactggt caaccttcgc cactaaattt gcgctggtta aaccgaagtc
    37261 cagtgatgta aaagcactgc tgggtcaccg tagtcgtagc gcagcagagt tgcgcgtgca
    37321 ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat caatttatcg acctgcagcg
    37381 cgttgatccg cctaagaaac cgctgaagat ccgcaaaatt gagtttgttg aaatttacga
    37441 gccgttttcc gaaggccagg ccaaagcgca ggctgaccgc tgcctgtcgt gcggcaaccc
    37501 atactgcgag tggaaatgcc cggtacacaa ctacatcccg aactggctga agctcgccaa
    37561 cgaggggcgt atttttgaag cggcggaact gtcgcaccag accaacaccc tgccggaagt
    37621 atgcggacgt gtctgcccgc aagaccgtct gtgcgaaggt tcctgcaccc tgaacgatga
    37681 gtttggcgct gtgaccatcg gcaacattga gcgttatatc aacgataaag cgttcgaaat
    37741 gggctggcgt ccggatctgt ccggcgtgaa acagaccggt aaaaaagtgg cgattatcgg
    37801 cgctggcccg gcgggtctgg cgtgtgcgga tgttctgacg cgcaacggcg taaaagcggt
    37861 cgtcttcgac cgtcacccgg aaattggcgg attgctgacc ttcggtattc cggctttcaa
    37921 gctggaaaaa gaggtaatga cgcgccgccg tgaaatcttc accggcatgg gtattgaatt
    37981 caaactcaat accgaagtgg gccgcgacgt gcagctggac gatctgctga gtgattacga
    38041 tgccgtgttc cttggcgtcg ggacttatca gtcaatgcgc ggcgggctgg aaaacgaaga
    38101 cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc aacaccaaac agttaatggg
    38161 ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa ggcaaacgcg tggtggtcct
    38221 tggcggtggc gacactgcga tggactgcgt gcgcacgtct gtacgccagg gggcaaagca
    38281 cgttacctgt gcctatcgtc gtgatgaaga gaacatgccg ggttcccgcc gcgaagtgaa
    38341 aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc cagccgctgg gtatagaagt
    38401 gaatggcaac ggcaaagtca gcggcgtaaa aatggtgcgc acagaaatgg gcgaaccgga
    38461 tgccaaaggc cgtcgccgcg cggagattgt ggcaggttcc gaacatatcg ttccggcaga
    38521 tgcggtgatc atggcgtttg gtttccgtcc acacaacatg gaatggctgg caaaacacag
    38581 cgtcgagctg gattcgcagg ggcgcattat cgccccggaa gacaacgaca acgctttcca
    38641 gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg atctggtggt
    38701 gaccgctatt gccgaaggtc gtaaggcggc agacggtatt atgaactggc tggaagttta
    38761 agcctgtccc agcgccgcct cctcagaaaa gagtcggcgc tgaaattctc ctttctttct
    38821 ggaaagcgcc tcgcataatc tgaagaaaac caatgtaata cgctcatctg gattgttacc
    38881 ggtagatgcc cggcgtatag ctgattccat tattcaacgc agcgccatat aaagggacag
    38941 ataatggaat cgctctcaga gagaacctca acaggctacc agcaaatcca cgacggtatt
    39001 attcacctgg tcgatagcgc ccggacggaa acggtacgta gcgttaacgc gctgatgacc
    39061 gcgacatact gggaaattgg ccgacgaatt gtcgaatttg aacaaggtgg cgaggccagg
    39121 gctgcgtatg gtgcgcagct aatcaagcga ctatcaaagg atttaagtct aaggtataag
    39181 cgtgggttct ctgcaaaaaa cttacgccaa atgaggcttt tttacctctt ttttcaacat
    39241 gttgaaattc gccagacagt gtctggcgaa ttaacaccat tgggaattcc gcagacacca
    39301 tctgcagaat tcccatcggc aaagatttgg cagacgctgt ctgccaaatc tttccccctg
    39361 ccctggtcca cctacgtccg tttactttcc gttaaaaacc ctgatgcccg cagcttttat
    39421 gaaaaagaga cgctccgctg tggttggtct gtccgccagc tagagcggca aattacgacc
    39481 caattttatg agcgaacact actgtcacat gacaaatcag ccatgctgca acaacacgct
    39541 cctgccgaga tgcatattct tccgcaacag gcgatacgcg atccctttgt gctcgaattt
    39601 ctggagttga aagatgaata ctcagaatct gattttgagg aggcgctgat caaccacctg
    39661 atggatttca tgctggaact tggggatgat tttgcctttg ttggtcggca gcgaaggtta
    39721 cgcattgatg acaactggtt tcgggtcgat ctgctgtttt tccaccgccg tttacgctgc
    39781 ctgctaatcg tcgatctaaa agtgggcaaa ttcagctata gcgatgccgg acagatgaat
    39841 atgtatctca actacgccaa agagcactgg acactaccgg atgaaaatcc gcccatcggt
    39901 ctggttctct gtgcagagaa aggagccggc gaagcgcatt atgcactggc tggtttgcct
    39961 aacaccgttc tggcgagtga atataagatg caactacctg atgaaaaacg actcgcggat
    40021 gaactcgttc gaacacaggc ggtgttagag gaaggctata gacgccgtta atttcagcgg
    40081 tttcgaattc tccagacact gtctggagaa tctttaaaac gaactaagtt ttaagccatt
    40141 aaatcagcct taaccttcac cacaaccttt ttaatctcac caggttcgcc gacaacgcat
    40201 cctggtttat gcggttctcc cggcataaac acggcgaaca ttcccggttt taagatgatg
    40261 gcttgctcgt tctcaatggc gctgcaaagc tggtaatcat cctcatggtg gaactcttca
    40321 cactgacgcg cagtgcctgc cgtgccaaac agaatccgtt cctcaccgtt taataacagc
    40381 tggatatcaa tgtattgctc gtgcagctcc gcttttttct caacgggcga ttgagtgtta
    40441 aacgtcatga cattcataaa gatattgtcg cactgtaatt cgtaacgacc cggtgctttt
    40501 tcttgcggcc tggcggctaa ggctagtgtt aacgcgtcct gtaacgcagg atgcaatccg
    40561 gcagacggta acgactgtac ttcacccatt atcataattt ttctccctgg gccaacaacg
    40621 cagccccaag taaacctgca tcgtggcggt agtgcgccgc cagtaaatca acatgaaatg
    40681 ccgctggctc ctgcgccaga tacgtttcca ccaacgccag atacccttct gccagaccaa
    40741 cgctgccacc gaccactacg cactggcaat cagttgtggc tttaatatca gcgatcagcc
    40801 ttgccagcac atgtgcggag cagtgaatca gttgctgcgc ctgctcatcg ccctgcccgg
    40861 cgcgtgtgaa aatagttctc gcatccgcgc ccgccagttc accctgcgct gccgctgcaa
    40921 taccgcgacc agaagcaatc gcttccacac aaccagtgcg tccacagccg cagactggac
    40981 catgaggatc agccagcgta tgcccgatgt gccccgccag accgccaggg ccggtgagca
    41041 gtttgccgcc gctcactaca ccgccgccaa caccagtgga aacggtgata aacaccatct
    41101 cggttatatc gccttccagc gcctgatact ccgcccacgc tgcggcctgc gcgtcgttaa
    41161 tggcaatggt cggcaaatcg gtaagttgtt ccaaagtttt gactaacgga aagtgtagca
    41221 atccaccgag attatgcgga ttcagcgcca gcaggctgcc gtcgcgaata attccggttg
    41281 aagcgatagc aacccgctgc gcatgaactt gcaacggaga gactaatgcg gataaggcat
    41341 catgcaaggc ttctggtgtc tggctggctg gcgtaggaag ttcacgacga tcgcggatct
    41401 gcccatcagc gccaatcagc gcggcggcaa gtttagtacc gccgatatca atcgccagtg
    41461 tggtcatagc accgcctttt tcatcgctgt gttgtaccac tgacaaatgt gttcaagacg
    41521 cgtaatggca gaaccgaccg tcaccgccca cgcgccgtgg cgcatcgcat ctgccgcctg
    41581 agcaggcgtg ttgtaacgcc cttcggcaat cacccgacat ccggcgtcgc tcaacgtttt
    41641 caccagcgcc agatccggct cttctggcgt ttcaggcgtg gtatagccag aaagcgtagt
    41701 gccaataatt tcggctccca gcttttggca tgccaggccg tcttccggcg ttgagcagtc
    41761 ggtcatcgcc agtaaaccgt gatggtgaat acgtgccagc agcgtttcaa caggcaccgg
    41821 acgcggtcgg tcggtgccgt caatggcgat aatgtccgcg cccgcctgcg ccagcgcatc
    41881 aacatcttca atataggccg tgatgcgtac cggagaatcc tccagatcgc gtttcacaat
    41941 cccgataatt ggcacgctca ccaccgcacg cgtggcttgc agatttgcca caccttcaat
    42001 gcgaatggca accgcgcccg cctgttctgc cgctaatgcc atggcggcga cgatttcggg
    42061 tttatcgagc gggctgtccg gaaccggctg gcaggagaca atcaggccac cgttagcagc
    42121 gattttttga tccagttgtg caagtaacga catacatctt cccttagcga aaggcccggt
    42181 acagagaccg ggcaacagga ttaacttttg gttttgacta aatcgttttt ggcgcggcca
    42241 aatggcacgg caccgctaaa tggtttgccg tcgatagcgt catgagtacg caatgcttcc
    42301 gggcgcagcc aacgctgaac gcgggaaggc atatccagac caatcagcag gatcaccaca
    42361 aatgtcagac tgaacgagag cgatgccagc gcagtaccca gatccagacg ttgagcgatc
    42421 aacgcgccga tgattggggc cagtgcaccg cccaatgcgc cgacgttgta ggtaaagccc
    42481 aggcccgctg cacgctggtc ggtatcgaaa taaccgccaa tcagtttcgg taagatcccg
    42541 gcgatccctt gtccaagcat ttgctggaag aacagtaaca gaccgagcac ccagacgttt
    42601 gcgccgccaa tcgcaaatac cggaataatc agcagctgcg aggccagcag actacaaacg
    42661 tacgctttgc gggttcccag ccagtcaccg aggaagccac cgacgcagca ccccaccgcc
    42721 gcgccaaagc cactaaagaa cagcacattg gcgacagtat gcgggtcata agccagatcg
    42781 gttttcagat acgttggcag cagcgcctga atcggccatg agtagaggaa agcaaacaac
    42841 acgaccacca tcagcattac gcccgttggc cagcgtttgc ccgtactctg caccataaag
    42901 ctgataaaga ttgcggcgca taacagcccg agtacagcga cgattgcggc attttgcagg
    42961 ttaccggcga agcagaacca cagcgcagta gccgccgcca gcgtcattac gatattggca
    43021 atgcgatgtt caccacggta aagaatgtct accatcgtgc gtactggtgc tttacccccg
    43081 tgtttctctt tccagtcttc cgcttccggg atgtttttac gcagccagag agcaaagatg
    43141 attggcaaaa tgccgataaa gaacagcgct ctccagcccc agaccggaac caccagacta
    43201 tagacctgag cggcaacgac ggcccccaca gagaagcctg aaatcaaaaa accactggct
    43261 ttgttacgca gatgttttgg ccagctttca atgacatagg tggcgctgga accgtattca
    43321 cccgccatcc ccatgccgat gaccagacgc gcgataaaca tggtgatgta gcctggcgca
    43381 aagccgcagg ccagcgtccc ggccgagaag agaacgatgc tggtgaccat tgccagacga
    43441 cgcccgtagc ggtcacccat agcgccgagc atcaggccgc cgaaccagcg agagataaag
    43501 gctgcggaga tcagacttgc cgcctgcacc gtcgtcagcc cgaattcacc ctgtacttcg
    43561 gtgagtacca gggcgattaa aacgaaatca aaaccgtcaa gcagatatcc caaccaggca
    43621 gcggaaaatg cgcgccattg tgcacggttg agatggcgat accacgggat attctgggtt
    43681 gtagtactca ttgtgagtct cccgcggtgg gcgatgccca cacgctttgg tatgaaaatt
    43741 gtagggtaca gatgcgttta tttcccctca cccggtaggg gcgagcgagg ggaaaacaac
    43801 tcacccgcgc tcttgcatca actgctgggc cagcgccttc agttctggca gatatttttc
    43861 atctaccggt ccaaacggtt tgcggcacag cggcacagaa acgacatcca tataatgcag
    43921 gacagttttc aggccgcgga atacgcccgt tttgatcagt aaatcaatga ctttgttgca
    43981 ctcagtttgc agtttctgcg cggtctggat atcgccttct ttcagcgcct tcacgatccc
    44041 ctgatatcgc cagcccatga tgttgtaagt actgccgata ccaccatcag cgcctgccag
    44101 caagccggaa gcgaagattt cgtcgtaacc gttatagagc accagatcag gatgttcacg
    44161 acggatctgt tccatctgat agagatcgcc agaagtctgt ttcagcgcac cgacgccagg
    44221 caacgtaacc agggtgttga tctgatccag ggtcagtttt accccactca gggctggaat
    44281 gttgtacacc accatcggca aaccatccgc cgaatcaata attgcccgat agtgatcgca
    44341 gtgttcttca aagctgaaag gatagtagaa cggcgtgacg gcggagacgg catcgaagcc
    44401 ataacgttta gccgatgccg caagttgttg gctttcggcg gtgctgacgc aaccgacgtg
    44461 ggcgatgagt ttaatcttac ctttcgcctc ttcagcgacg atttccagta cctgttcacg
    44521 ctcggaaagg ctttgtacaa aggcttcgcc ggtagaacca cccacgtata aaccgtcgat
    44581 gccctgctga atattgaact gaaccaggcg acgcagactc gctttatcca gtgcttgttg
    44641 ttgatcaaaa ggagtcagga gtgcggccat tacgccacgt aaattcgttg ccataaatac
    44701 ctctgaagtg atgcttatct gataaacgat atacctttat acctgttata ccagatcaat
    44761 taagcaacac cccatacaga aagcttataa tgcgatctgc ttcactaaag tggcattatt
    44821 tctttttgtt ggtggtctga ccgaaggcgt gccaggtagc agagacgctg ttgagatgcg
    44881 attgcaacgc acgatcggct tcatcgggat cgtggcggcg gatcgcgtca acgatcgcaa
    44941 tatgctgttg atagctaacg ttgttatgct cgtgcagagc ctgatcagcc acggttggac
    45001 gtgcggcgat cagccagtcg agcaaggcaa cgtggatcgc catgaagatt gggttaccgg
    45061 ggatctccgc cagcacgcgg tggaaatcaa cgtctgaacg aatgaatgcc gcgttgttat
    45121 ccagcgactg actgttgatt tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg
    45181 cgtgttcagc cgcatagcgc accaggctgg attcaaagaa cagacgtaat tgttcgaaat
    45241 gggcaatccc gccaggatga gaaaggaaat ctttcgccat gccggaaagc tcgccgatga
    45301 tggtgtccgc agaaggacgt gagacgcgag cgcgttcgcc gttgtttatc tgcaccagac
    45361 ctttgcgttt taacgctgcc agcgcttcgc gcaccgaagg acgcccgacg ttaaagaacg
    45421 ccatcagttc gcgttcagac ggtaattgtt caccttcgcc aaattcacga cggcggatca
    45481 tctgttccag ctcttcttcc accatttcgg agagtttttt acgcgccagc gggcggctgc
    45541 gtaagttgcg gccaattgca ggtgaagaat cttcggtttg cgaatcaaat gcgttcataa
    45601 ggcccattct gtaaggtcag tgtgattaac atcatcagtg acatcctatc acaggattga
    45661 aagtaggggg aaatggcggg gttttctctt tgtgcctcat cattactata attaactgaa
    45721 taattaacta ttgcgaaaaa ttaatgtaac gcagataaaa ac
//
LOCUS       DAZUCC010000013        45678 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZUCC010000013 DAZUCC010000000
VERSION     DAZUCC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.013"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(341..883)
                     /gene="ycfP"
                     /locus_tag="ACHY09_000623"
     CDS             complement(341..883)
                     /gene="ycfP"
                     /locus_tag="ACHY09_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFS3883639.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDICQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(906..1931)
                     /gene="nagZ"
                     /locus_tag="ACHY09_000624"
     CDS             complement(906..1931)
                     /gene="nagZ"
                     /locus_tag="ACHY09_000624"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFS3883640.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAREAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHSDPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(1942..2766)
                     /gene="thiK"
                     /locus_tag="ACHY09_000625"
     CDS             complement(1942..2766)
                     /gene="thiK"
                     /locus_tag="ACHY09_000625"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFS3883641.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2747..3388)
                     /gene="lpoB"
                     /locus_tag="ACHY09_000626"
     CDS             complement(2747..3388)
                     /gene="lpoB"
                     /locus_tag="ACHY09_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFS3883642.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPIEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3402..3779)
                     /locus_tag="ACHY09_000627"
     CDS             complement(3402..3779)
                     /locus_tag="ACHY09_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFS3883643.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTLPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3782..4141)
                     /gene="hinT"
                     /locus_tag="ACHY09_000628"
     CDS             complement(3782..4141)
                     /gene="hinT"
                     /locus_tag="ACHY09_000628"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFS3883644.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4475..6664
                     /gene="fhuE"
                     /locus_tag="ACHY09_000629"
     CDS             4475..6664
                     /gene="fhuE"
                     /locus_tag="ACHY09_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFS3883645.1"
                     /translation="MLSTQFNRDNQYQTTSKPSLLAACIALALLPSAAFAAPATEETV
                     IVEGSATTPVDDENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWAYIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6724..8157)
                     /gene="ptsG"
                     /locus_tag="ACHY09_000630"
     CDS             complement(6724..8157)
                     /gene="ptsG"
                     /locus_tag="ACHY09_000630"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFS3883646.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8452..9249)
                     /locus_tag="ACHY09_000631"
     CDS             complement(8452..9249)
                     /locus_tag="ACHY09_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS3883647.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPQDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9260..10264)
                     /gene="holB"
                     /locus_tag="ACHY09_000632"
     CDS             complement(9260..10264)
                     /gene="holB"
                     /locus_tag="ACHY09_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS3883648.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFHGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10261..10902)
                     /gene="tmk"
                     /locus_tag="ACHY09_000633"
     CDS             complement(10261..10902)
                     /gene="tmk"
                     /locus_tag="ACHY09_000633"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS3883649.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATLPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(10892..11914)
                     /gene="yceG"
                     /locus_tag="ACHY09_000634"
     CDS             complement(10892..11914)
                     /gene="yceG"
                     /locus_tag="ACHY09_000634"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFS3883650.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKLRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11917..12726)
                     /gene="pabC"
                     /locus_tag="ACHY09_000635"
     CDS             complement(11917..12726)
                     /gene="pabC"
                     /locus_tag="ACHY09_000635"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFS3883651.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12846..14087)
                     /gene="fabF"
                     /locus_tag="ACHY09_000636"
     CDS             complement(12846..14087)
                     /gene="fabF"
                     /locus_tag="ACHY09_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS3883652.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     VYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14175..14411)
                     /gene="acpP"
                     /locus_tag="ACHY09_000637"
     CDS             complement(14175..14411)
                     /gene="acpP"
                     /locus_tag="ACHY09_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS3883653.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14622..15356)
                     /gene="fabG"
                     /locus_tag="ACHY09_000638"
     CDS             complement(14622..15356)
                     /gene="fabG"
                     /locus_tag="ACHY09_000638"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFS3883654.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15369..16298)
                     /gene="fabD"
                     /locus_tag="ACHY09_000639"
     CDS             complement(15369..16298)
                     /gene="fabD"
                     /locus_tag="ACHY09_000639"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS3883655.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16314..17267)
                     /gene="fabH"
                     /locus_tag="ACHY09_000640"
     CDS             complement(16314..17267)
                     /gene="fabH"
                     /locus_tag="ACHY09_000640"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS3883656.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17335..18405)
                     /gene="plsX"
                     /locus_tag="ACHY09_000641"
     CDS             complement(17335..18405)
                     /gene="plsX"
                     /locus_tag="ACHY09_000641"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS3883657.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18486..18659)
                     /gene="rpmF"
                     /locus_tag="ACHY09_000642"
     CDS             complement(18486..18659)
                     /gene="rpmF"
                     /locus_tag="ACHY09_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS3883658.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18711..19232)
                     /gene="yceD"
                     /locus_tag="ACHY09_000643"
     CDS             complement(18711..19232)
                     /gene="yceD"
                     /locus_tag="ACHY09_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFS3883659.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19431..20015
                     /gene="yceF"
                     /locus_tag="ACHY09_000644"
     CDS             19431..20015
                     /gene="yceF"
                     /locus_tag="ACHY09_000644"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFS3883660.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20128..21087)
                     /gene="rluC"
                     /locus_tag="ACHY09_000645"
     CDS             complement(20128..21087)
                     /gene="rluC"
                     /locus_tag="ACHY09_000645"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFS3883661.1"
                     /translation="MKTETPSVKIVAINADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21660..24845
                     /gene="rne"
                     /locus_tag="ACHY09_000646"
     CDS             21660..24845
                     /gene="rne"
                     /locus_tag="ACHY09_000646"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFS3883662.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERAEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVAETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            24979..25272
                     /locus_tag="ACHY09_000647"
     CDS             24979..25272
                     /locus_tag="ACHY09_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFS3883663.1"
                     /translation="MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            25269..25763
                     /locus_tag="ACHY09_000648"
     CDS             25269..25763
                     /locus_tag="ACHY09_000648"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3883664.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIDR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(25858..26811)
                     /gene="flgL"
                     /locus_tag="ACHY09_000649"
     CDS             complement(25858..26811)
                     /gene="flgL"
                     /locus_tag="ACHY09_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFS3883665.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26823..28466)
                     /gene="flgK"
                     /locus_tag="ACHY09_000650"
     CDS             complement(26823..28466)
                     /gene="flgK"
                     /locus_tag="ACHY09_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFS3883666.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDASGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDTGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(28532..29473)
                     /gene="flgJ"
                     /locus_tag="ACHY09_000651"
     CDS             complement(28532..29473)
                     /gene="flgJ"
                     /locus_tag="ACHY09_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFS3883667.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(29473..30570)
                     /gene="flgI"
                     /locus_tag="ACHY09_000652"
     CDS             complement(29473..30570)
                     /gene="flgI"
                     /locus_tag="ACHY09_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFS3883668.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(30582..31280)
                     /gene="flgH"
                     /locus_tag="ACHY09_000653"
     CDS             complement(30582..31280)
                     /gene="flgH"
                     /locus_tag="ACHY09_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFS3883669.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(31333..32115)
                     /gene="flgG"
                     /locus_tag="ACHY09_000654"
     CDS             complement(31333..32115)
                     /gene="flgG"
                     /locus_tag="ACHY09_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFS3883670.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(32286..33041)
                     /gene="flgF"
                     /locus_tag="ACHY09_000655"
     CDS             complement(32286..33041)
                     /gene="flgF"
                     /locus_tag="ACHY09_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFS3883671.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQTTRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(33061..34266)
                     /gene="flgE"
                     /locus_tag="ACHY09_000656"
     CDS             complement(33061..34266)
                     /gene="flgE"
                     /locus_tag="ACHY09_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFS3883672.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMTLVFNANGVLTSDPTANISTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(34291..34986)
                     /gene="flgD"
                     /locus_tag="ACHY09_000657"
     CDS             complement(34291..34986)
                     /gene="flgD"
                     /locus_tag="ACHY09_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFS3883673.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGTTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34998..35402)
                     /gene="flgC"
                     /locus_tag="ACHY09_000658"
     CDS             complement(34998..35402)
                     /gene="flgC"
                     /locus_tag="ACHY09_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFS3883674.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(35406..35822)
                     /gene="flgB"
                     /locus_tag="ACHY09_000659"
     CDS             complement(35406..35822)
                     /gene="flgB"
                     /locus_tag="ACHY09_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFS3883675.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35978..36637
                     /gene="flgA"
                     /locus_tag="ACHY09_000660"
     CDS             35978..36637
                     /gene="flgA"
                     /locus_tag="ACHY09_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFS3883676.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            36713..37006
                     /gene="flgM"
                     /locus_tag="ACHY09_000661"
     CDS             36713..37006
                     /gene="flgM"
                     /locus_tag="ACHY09_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFS3883677.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            37011..37427
                     /gene="flgN"
                     /locus_tag="ACHY09_000662"
     CDS             37011..37427
                     /gene="flgN"
                     /locus_tag="ACHY09_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFS3883678.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(37467..39002)
                     /gene="murJ"
                     /locus_tag="ACHY09_000663"
     CDS             complement(37467..39002)
                     /gene="murJ"
                     /locus_tag="ACHY09_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFS3883679.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(39112..40035)
                     /locus_tag="ACHY09_000664"
     CDS             complement(39112..40035)
                     /locus_tag="ACHY09_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS3883680.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(40037..40684)
                     /locus_tag="ACHY09_000665"
     CDS             complement(40037..40684)
                     /locus_tag="ACHY09_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFS3883681.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVMDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(40695..41279)
                     /gene="rimJ"
                     /locus_tag="ACHY09_000666"
     CDS             complement(40695..41279)
                     /gene="rimJ"
                     /locus_tag="ACHY09_000666"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFS3883682.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            41515..42723
                     /gene="mdtH"
                     /locus_tag="ACHY09_000667"
     CDS             41515..42723
                     /gene="mdtH"
                     /locus_tag="ACHY09_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFS3883683.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            42787..43434
                     /gene="grxB"
                     /locus_tag="ACHY09_000668"
     CDS             42787..43434
                     /gene="grxB"
                     /locus_tag="ACHY09_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFS3883684.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPIELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            43568..44128
                     /locus_tag="ACHY09_000669"
     CDS             43568..44128
                     /locus_tag="ACHY09_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS3883685.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            44234..45280
                     /gene="pyrC"
                     /locus_tag="ACHY09_000670"
     CDS             44234..45280
                     /gene="pyrC"
                     /locus_tag="ACHY09_000670"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS3883686.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVRKEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            45354..45599
                     /gene="dinI"
                     /locus_tag="ACHY09_000671"
     CDS             45354..45599
                     /gene="dinI"
                     /locus_tag="ACHY09_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFS3883687.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11023 a        12333 c        11393 g        10929 t
ORIGIN      
        1 attaacaaca ggttacagca atgaaaatgt tcttcactat tgagaataac atgaatggtg
       61 cattggtcat accaaaattg atgttaatca agttttgttg ctgaaaagat aggcgacttt
      121 accgggcgag atcaaaaaac agagtttatt gtttattatt tgagcgaatt tgtgagagtg
      181 atatagagtg aggggtgtat ggagtgcgtt cggtaggcct gataagaggc gcatcaggca
      241 ttgatgctcg actgtcggat gcggcgtgga cgccttatcc gacttacaga attcggagcg
      301 catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg
      361 ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc
      421 agacaatctc gtaataatga tgcagctctt cagatgtccg ctggctgtta agagcttcat
      481 cattacgcga caaaatcacc aaacaacgat cgcgattctt ttcgcggaag ttggtcacac
      541 acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat
      601 aagggaacaa attagggttg aagatcacct ggcagatatc gcaaagaaaa ccaatccgtt
      661 ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg acgctcgtcg acgttcagtt
      721 gcaacatttt gtccacttct ttgagcagat gctgcatatc atgtttcgga tgccgcgtac
      781 tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt
      841 tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc
      901 cagggttagt gacctgcttt ctcttcctgc cagcgttcat gtaactgatt cagacgggcg
      961 ctgatcgctt tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga
     1021 tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc
     1081 cctttacgat tattgcagac caggatcata tcgcaacccg cgtccagtga tgcctgcccg
     1141 cgttcggcat aactgcccat aatcgcggct ccttccatcg ataaatcgtc agagaaaatc
     1201 acgccatcaa aacccagttc ctgacgcaaa acggttttca gccaataggg agaaccgctc
     1261 gccggacgcg gatcgacatc actgtagatc acatgcgcag gcataatggc gtcgagttta
     1321 ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgcgga
     1381 cgtgggtcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag
     1441 tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg gctggcaatt
     1501 gccagggctt tttcgggatc ggaatgataa gaacgctcgc caatcgccgc gctgatatgc
     1561 ccgacgtcca gcactggcgc aaagctgata tcaatatcca tagcaatcat ttcgctggcc
     1621 atcaaccaac ctgcctctcg cgccagtttt ccgccttctt ccattcccaa cagcgcagca
     1681 aatgattgtg ccgctggcaa gcgggtaaaa ccttcacgaa aacgctgcac gcgtccacct
     1741 tcctgatcaa ccgccaccac cagatgattg cgcgaagctg cgcggatctg gcgcaccagt
     1801 tcacgtaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc
     1861 ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt aaccttcgac atccaacatt
     1921 actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc
     1981 cagcctgata aattgttgat cgccggtttg tcgccagcgg tactcaaacc accctgcttt
     2041 gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat
     2101 cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tggtcagtat tttccaccca
     2161 caccgccgcc agttccagcg cgatatcacc atctccggca tactcccagt cgatgagttt
     2221 taaccctgac gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa
     2281 gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt aaccttcgca gccaacccac
     2341 tgtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaacggca acagcgttat
     2401 tcgccagcca aaacgcggtt gttgatgtag ataatacagc aagcctgcca gttcgttggt
     2461 atccggcaaa tacgttttta cctcgccggg cagatagtcg accaccatcc agtcacggag
     2521 atataaatgc ggcttcggtg caatgcatgc gggtagttgt gataaagccc gatactggcg
     2581 caagaacgcg aaccgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc
     2641 ctgatgttca atgagaaaac tcccgccact aagcccacta ttaaacgtcg tgacgggatg
     2701 aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa
     2761 cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgaa
     2821 gggtcggagc gttaacattg ccagaggcgc tggagtacag cacgtaatga gcgccgacat
     2881 tgcgggcaat gcctatggct ttactacggg tgcctaaact gtcctgcggc gacaaaccta
     2941 actgttgctt tgccatcgac agctgctggg cggaaaccag ggtaaattta ccgttattag
     3001 ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt
     3061 tgttaacgct atcaaccagc aggacgctac ccgcagtcac gccgtcagcc ccaagcatct
     3121 tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg
     3181 gtgcagtttg atcttcgtgc tcaattgggc ctggctgctg cggtatcgtc ggcaccgatg
     3241 gcactgtggg gacaggctgt tgcggctcgg ctggttgttc cggcgctggt ttcacttctt
     3301 ctatcggcgc aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa
     3361 tcaaggcgta gcgactcatt ttggtcatca agattcaccc cttacaaata tagataaagt
     3421 ctgactttgt gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg
     3481 ggaagggtaa cgctgcgtgg cctttccaga ggatgcatct ccagccctct ggcgtcatac
     3541 cagtaaaaac gataatgaac ggtaacgggt tcttgccttt cgttataaag cgttgaggat
     3601 gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc
     3661 aataaagatg actccatcac cagcgattgc tcatcattca ccggaatttc cggatgtgaa
     3721 cgacagccca ccagcactaa caacgccaga gacaccagcc caaagcatcc ttttctcatt
     3781 gttacagacc tttatgcgcc agcattggtc ccagtggacg gccacccaac aaatgcatat
     3841 ggatgtggta aacctcttgt ccgccatggc ggttggtgtt catgatcagg cgatagccat
     3901 cttcggcaat accttcttgc tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg
     3961 cctgctcatg ctcagctgac acgtcgttca cagtcggaat gaggatattc ggaatgatca
     4021 gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt
     4081 agacgatatc ggaggggatc tcacgacgaa taattttgct gaatatagtt tcttctgcca
     4141 cgacattttc ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg
     4201 agtatagagt atggtcgagt taaacctgct ctttcaacct taactcacga tttcttaagc
     4261 aaaaaatcgt tttgctgccg tatatatcgc cattattccc atttctgcct gcgaatgcgc
     4321 tgaaaagaca ttctctgttt ggtttacacg tcaaatatga atgcgtatat ttctcatttg
     4381 catttacatg caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat
     4441 gagcctgacg acaacataaa ccaagagatt tcagatgctt tcaacacaat ttaacaggga
     4501 taatcaatat caaaccacct ccaaaccgtc actgcttgcc gcctgcatag cactggcact
     4561 cttaccttcc gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc
     4621 agccactact ccagttgatg acgaaaatga ttacagcgta acgtctacct ctgcgggtac
     4681 caaaatgcag atgactcaac gtgatattcc tcagtcggtc actattgtta gtcagcagcg
     4741 gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag
     4801 caaaagccaa gcggattccg atcgtgctct ttattattcc cgcggattcc agatcgataa
     4861 ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact
     4921 ttctgatatg gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac
     4981 cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga ccagtcgtga
     5041 atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga
     5101 tttacaaagc ccactcaccg aagatggtaa aatccgcgca cgaattgtcg gcggctacca
     5161 gaataacgac tcatggctgg accgctacaa cagtgaaaag accttcttct ccggcattgt
     5221 cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga
     5281 tgttaatagc ccaacctggg gcggtttacc gcgctggaat actgatggca gcagcaacag
     5341 ttacgatcgc gcacgcagta ccgcacctga ctgggcgtat aacgataaag agatcaacaa
     5401 ggtctttatg accctcaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac
     5461 ccactccgaa gtcgaatttg acagtaaaat gatgtatgtc gatgcctatg taaacaaagc
     5521 ggatggtatg ctggttgggc catacagtaa ttatggacct ggctttgatt atgtcggcgg
     5581 taccggttgg aacagcggca aacgtaaagt tgatgcgctg gatttgtttg ctgacggtag
     5641 ttatgaattg tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa
     5701 caatcgttac ttcagttcat gggcctacat cttcccggat gaaattggca gtttctacaa
     5761 ctttaatggc aatttcccac aaaccgactg gtcaccacag agcctggcgc aggacgatac
     5821 cacacatatg aaatcgttat atgctgccac tcgtgtcacc cttgccgatc cgctgcatct
     5881 gatcctcggc gcacgttata ccaactggcg ggttgatacg ctgacttaca gcatggagaa
     5941 aaaccacacc acgccttacg ctggtctggt gtttgacatc aatgacaact ggtcgaccta
     6001 cgccagctat acctctattt tccagccgca aaatgatcgt gacagttcag gcaaatatct
     6061 ggctccaatc accggtaaca actacgagct gggtctgaaa tcggactgga tgaatagccg
     6121 tctgaccacc acgttagcca tcttccgtat tgagcaggat aatgtcgctc agtccaccgg
     6181 tacacctatc cccggcagca acggcgaaac cgcctataaa gcggtggatg ggacagtcag
     6241 taaaggtgtg gaatttgaac tcaacggcgc aattaccgac aactggcagc tgacgtttgg
     6301 cgcaacgcgc tatattgcag aggataacga aggaaacgcc gttaatccta atctgccacg
     6361 caccacggtt aaaatgttca ccagctaccg gttgcctgtc atgccagagt tgacggtcgg
     6421 cggtggtgtt aactggcaaa atcgcgtgta taccgacacc gtgacaccgt atggcacctt
     6481 ccgcgccgag caaggtagct acgcgctggt ggatctcttc acccgctacc aggtgacgaa
     6541 aaacttctcg ttacagggga atgtcaataa cctgttcgac aaaacctacg ataccaacgt
     6601 ggaaggttct attgtctacg gcgcaccgcg taatttcagc attaccggca cgtatcaatt
     6661 ctgaataaca cctgtaaaaa aggcagccat ctggctgcct tagtctcccc aacgtcttac
     6721 ggattagtgg ttacggatgt actcatccat ctcggttttc aggttatcgg atttagtacc
     6781 gaaaatcgcc tgaacaccag aaccagcaac cactacgccc gctgcgccca gtttcttcag
     6841 gccagcctga tccactttag acacatcagc aacgctgacg cgcagacggg taatacatgc
     6901 gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca accagagccg gtgccatttc
     6961 gctggtacct gtcgcttttg cgtcttcagt cgcgtcttca cgacccggcg ttttcagatc
     7021 cagtgcttta atcagcacgc ggaagatggt gtagtaaaca atggcataac cgataccgac
     7081 gatcgggaac agccacagtt tgctgctgtt accagacaga acgatgaagt cgatcagacc
     7141 gtgcgagaac gacgtaccgt cacgcatccc cagaagaata cagattggga atgccaggcc
     7201 tgccagaatc gcgtggatga tgtacaggat cggcgcaacg aacatgaagg agaactcgat
     7261 cggctcggtg ataccggtca ggaacgaggt cagcgccgcg gagatcataa taccgcccac
     7321 tttcgcgcgg ttttctggtt tagcagagtg ccagatagca attgcggcag ctggcagacc
     7381 gtacattttg aacaggaagc caccagacag tttacccgca gtcgggtcac ccgccatata
     7441 acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac caatctgcat
     7501 ctggaaaggt acgttccaga tgtggtgcag accaaacggt accaggcaac gttcgatgaa
     7561 accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag agaaggtctg
     7621 gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa agatggcagc
     7681 caggcctgaa atgatcggca caaagcgttt accggcaaag aagccaagat actcaggcag
     7741 cttaatacgg tagaaacggt taaacatgta cgctgcgatc gcaccggaga taatcccccc
     7801 gagtacgcca gtatccgcca ggtgtttaga ggcgatttct tcagcaggta aatgcagtac
     7861 cagtggcgca accacggcca tggttttaac catgatgcca taggcaacaa ctgcggccag
     7921 cgcggatacg ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa aaatcagtgg
     7981 catgtttgca aagacggaac cgcctgcttc tgccataaca tgcgatacaa cggcgggcag
     8041 ccagctgaaa ttcgcggaac cgacgcccag cagaatacct gcgataggca gtacggatac
     8101 cggcagcatc agcgatttac cgaccttttg caggttagca aatgcattct taaacataat
     8161 tgagagtgct cctgagtatg ggtgcttttt ttacgttttc acgcgtggca aggggggaga
     8221 gcctcgccgt gtacagggca tctaagcgcc ctttatttat tacacagagt aaaataattc
     8281 agtgccgatt tgtttgacgg ctatcacgtt tcaacttcag atcattggag aagttgcaca
     8341 ttttgacaaa acatatgtca caatatttca aacgtggtta ttcaccgccc aattttaggc
     8401 ggtgaacttt actcgtttta gccattaatt acgagcttta aaaaaactca ttcaacggat
     8461 ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg aagttatcgg tggttacctg
     8521 cgccagttct tcaacggcaa cacctttcaa cacagccatg tattctgcaa cgtcacgaac
     8581 catcgcaggt tgattctctt ttcctcgatg cggaaccggt gcaaggtaag gtgagtcagt
     8641 ttctaccagt aaccgatcct gggggacata acgcgcggca tcgcgcagtt gctccgcatt
     8701 acggaaggtc acaatgccgg aaaaggagat gtaaaatccg agatccagta atttacccgc
     8761 cgtttctctg tcctctgtaa aacagtgtag tacgccaccg caatccgtca ctttttcttc
     8821 gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta tggacgataa ccggcttgtt
     8881 cagttcacga ccaatctgga tatgatggat gaaggattct tgctgccgta ctttagtttc
     8941 cggcgtgtaa taataatcca acccggtttc acccagcgca acaacgccct cttctgccgc
     9001 cagacggcgt aaatcttcta catcgtaggg atcattctgg ttgagtggat gcacgccaca
     9061 agaaaatacg acgttgtcac gttcgcctac cagatcccgc atgtgtaaat aacccggtaa
     9121 tgttgtggcg actgccagac aaaatttcac atcgcgtgcg gcggctttcg ccagaacgtc
     9181 atccacgtcc ttatgcaaag attcataatc cagaccatcg agatggcagt gtgagtcgac
     9241 taaaaacatg atgtctctct taaagatgag gaaccggtag cacaacgccc ggttgcaggt
     9301 aatgctcaat acgcagtaaa agatcggtga tgagaagctc gcggttaata cctgtgacag
     9361 acattaactg ttcacgaatg tggcaaacat cccccagtat agcctgcagg cgcgagggag
     9421 aaagatggtt tgccagttcg acgaccaggc ccggcacatc aacattggtc acctgcgcag
     9481 cgccatgatg gcgttttagc gcatccatca gcaacgttgc cagccagtgt aaacgcgccg
     9541 gagcttgttc atgattaagg gccgctagca gcgaatacca gtcgcccgat ggcacgctat
     9601 atgccaaagc ctgacacaat gtttcacgag cctgccagtt atctccgtga aacaacgcca
     9661 gtgccgcgcc aggcgaaccg gcacttaagc gcaatgcggc aagtaatgca tcctgtgaca
     9721 ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc cggcggcggc gcaaggtaat
     9781 gtaaccgaca acgactacgt aatgttgcca gtaaacgttc tggctcgcgg gtagcaagga
     9841 aaaaccacgt ttctgctggt ggctcttcaa gcgttttcag caatgcgtta gccgcggcgt
     9901 cggtcagtaa ggcagcatcg gttacccaga caactttcgc accacctaag cgtgcgtgct
     9961 cattcagctt ttcggtgacc tcacgtaccg catcaatgcc cagcgtattt ttcccttttt
    10021 cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg catcaactga catccacgac
    10081 agtgaccgca acttttgtgg ccctgcggtt gttggcagag taaataacgg ctcagggcgt
    10141 agattaaagc atcatcgccc atgcccggta acgcctgaat gagcagcgca tggtgacctc
    10201 ttccggcctg atagctggct accagttttt cgaaatcagg tcgtaaccat ggataccatc
    10261 tcatgcgtcc aactccttca cccagttggt cacggtagtg cggattgcat ccatcacagc
    10321 ctccagcggc agggtggcat caatggtatg aatgctttta tcttgtgctg ccagttccag
    10381 atagcgggcg cgggtgcgat taaaaaaatc gaaagactct tgctcaatac gatccagctc
    10441 gccgcgtgca cgcgcgcgtt ttaagccaac ttccggggta acatcgagat agagcgttaa
    10501 gtcggggcga aaatccccga gaacagcatc acgcagtgtt gccagcatat gttggtcaat
    10561 accacgtccg ccgccctgat acgcctgagt ggagagatcg tggcgatcgc caatcaccca
    10621 agtgccgtta gccagcgcag gtttgatgac cgtttctacc agttgaacgc gcgcggcata
    10681 aaacatcaga acttcggctt tatcggtaat gacttcatcg cctaccgatt tgatatccag
    10741 caccaggctt cttaactttt cggcaagttg cgtaccgcca ggttcccgag tgaaaaccat
    10801 gtcgcggata cccagttgct caagcgtctc aaccaccacg ttacgcgcgg tagttttgcc
    10861 tgcgccttcc agcccctcaa tgacgatata cttactgcgc atttttttcc ttaagcactt
    10921 tcagataatc ctgcacagac ttgttatgac tggcaagatt ggtattaaac gtgtgaccac
    10981 ctttaccatc ggccacaaaa tagagatacg gcgtttttgc cggatgcgca gcagccttca
    11041 gcgaatccgc ccccggcgtc gctatcgcac ctggcggcaa accggtaatg gtataggtgt
    11101 tatacgctgt cggcgtttcc aggtctgcac gagaaagttt gccattataa cgctctccca
    11161 tcccgtaaat cacggtcggg tcggtctgca ggcgcatacc aatgcgtaac cggttgataa
    11221 ataccgaggc aacctgatcg cgttcactgg caacggcggt ttctttttcg ataattgatg
    11281 ccatcgtcac caactggttt ttatctttat aaggcagacc gtccgcacgc ccttcccagg
    11341 cgctatcgac cgctttcacc attttcttgt gcgctcgctt gagtaacgcg acatcggtgg
    11401 tattggcggt atacatccag gtgtctggcc agaaccaacc ttcaatccac tccgggtttt
    11461 ccagttcaag tgcctgcgct acggtggcgt acttatcgtc gctcagcgtg tgcttgatat
    11521 agggggcctc acgcaattgc ttgaggtaat cgctcagacg catcccttct accagtcgca
    11581 gagggaactg tgcttcttta ccgctttcca gcaatttcag catctcgcgc acggtcatct
    11641 gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag atccggttcg atacgcagca
    11701 gccattggaa aacccgtggg cgattgatga tcttatcggc ataaagctgt tcaccgagcg
    11761 ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc ttctttgata agtaatttgc
    11821 tgtcggcaag atggcgaagc ttccagacgc ccacaccagc ggcgataccc agtaccacca
    11881 gcaataacaa gataatcaat aacacttttt tcatgactaa ttcgggcgct cacaaagtgg
    11941 ggctaaatat tcatataacg ttgctgacga aaaggagaca tcgccacagg cacgtacggg
    12001 catcactggc attaacgcat tacaaataac catctcatct gcctgcaacg cctcctccag
    12061 cgaggcttgc acttcgacaa gctgataaga ggattgtgcc agcaaacgga tacagaattg
    12121 tcgcataatg ccgttaacac ctgcctgatc cagtcgcggc gtataaacta cgttgccctt
    12181 ccgccagaac aaattagccg cacagcattc cgtaacccac ccttcgctgt caaggaccag
    12241 cgcctcatca gcgtttgtct gctcaagatg agagcggatc aatacttgct caagccgatt
    12301 aagatgttta atacctgcaa gatgaggatt gcgccccagc cgcaccgggc ttagcgccaa
    12361 cgtcatcccc tcgttacgca aacggtcgta atgtgcggga taaaccgtaa cggagagaat
    12421 ccgcgttgcc ggtccgctgt tcaatgtgct gtatcctcgc ccgccactac cgcgactgat
    12481 caccactttc agcacaccat tttgctgttc tgctgccagc gttttcatct ctagttcaag
    12541 ctgaggccag aagtcacagg aaatcatcaa ccgctgacaa gcatcctgta gtcgctggat
    12601 atgcgccgat aacaaactga ctttaccgtc gataactctg gcggtggtaa aacaaccatc
    12661 accaaactgc gttgcccgat cgcttactgc cagcgattcc tgcttatgac cgttaattaa
    12721 gaacataccg gctccttatc agagaattgt tagtgtggca gcatgttcac tacggaacaa
    12781 gtcggaataa aagctaagaa aaaaggcccg caagcggacc ttttataagg gtgggaaatg
    12841 acaacttaga tctttttaaa gatcaaagaa ccattagtgc caccgaagcc gaaggagtta
    12901 cacagcgtgt attccattcc gctgacctga cgtgcttcgt gcggtacgaa atccagatcg
    12961 caaccttcat ccgggttatc caggttgatg gttggcggaa cagcctgatc gcgcagcgcc
    13021 aggatggagt agatagattc tactgcaccc gccgcaccta acaggtgacc ggtcatagat
    13081 ttcgtggagc ttaccaacac acggcttgca gcttcaccga agatggtttt caccgcctgc
    13141 gcttcagctt tatcgccagc cggcgtagaa gtaccgtgag cgttaacgta gccaatctga
    13201 ctcgcttcaa tgcctgcatc acgcagagca tttgccattg ccagagctgc gcctgcgcca
    13261 ttttctggcg gtgacgtcat atgataagca tcgctgctca taccaaagcc gacgagttca
    13321 gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac cagcataccg
    13381 gcaccatcgc ccagtacaaa accatcacgc tctttatccc acgggcggct cgccgcttgc
    13441 gggttatcat tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac gcccagcggc
    13501 gtactggctt tctctgcgcc acctgcaacc atcacgtcag catcgccata cgcgataata
    13561 cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg cggtcgcgat agagatgctt
    13621 gggccacgca ggccatacat gatagtcaga tgacctgcca ccatgttcac aatcgttgac
    13681 ggaacgaaga atgggctgat cttacgtgga ccaccgttca tcagagatgt gtggttttct
    13741 tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg caccaatgcg ggttgcgttc
    13801 tcttccgtta tttcaaggcc agaatcctgc atggcctgaa cgccagcgac aattccatat
    13861 tgaatgaagg catccatctt gcgctgttct ttgcgcgaga taatgtcctc acagttaaaa
    13921 tcctttacta agccagcaaa tttcgttgca tagacgctag tatcgaaatg gtcgattagg
    13981 ctgatgccac tctgaccggc aagcagagct ttccaggtag actctacggt attgccgaca
    14041 ggagacaaca tgcccagtcc ggtcacaact acacgacgct tagacacgtt tgtcctccag
    14101 ggagggaaaa aatgattcta gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc
    14161 ctggagatgt tcacttacgc ctggtggccg ttgatgtaat caatggcagc ctgaacggtg
    14221 gtgattttct cagcttcttc gtccggaatc tcagtatcaa actcttcttc cagagccatt
    14281 accagctcaa cggtgtcaag agaatccgcg cccaggtctt caacgaaaga agcattgttg
    14341 gtaacttctt cctgcttaac gcccagctgt tcgccgataa ttttcttaac gcgttcttcg
    14401 atagtgctca tactcttaaa tttcctatca aaactcgctt tcgcgatggt tttcgtagtg
    14461 tataaaatgt tgaaaaattt gcaactaaat cccggcaggt cttaccacga ttttacgtta
    14521 ttttgaggcc ttacccccca ataacgcaaa tcattttgca ctaattatta ctcataacca
    14581 cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat gtacatcccg
    14641 ccgttcacat gcaaagtttc acccgtgatg taagctgctt cgtcggatgc caggaatgca
    14701 accgcgttgg cgatttcctg tgcgccgccg aggcgacccg caggaacctg cgccaggata
    14761 cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa gcccggagca
    14821 acaacgttta cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt actgaagccg
    14881 atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc catttcccat ggtaccaacc
    14941 acagaaccga tagtgataat acgaccatga cgctttttca tcatagcgcg cattaccgct
    15001 tttgacagac ggaaaacaga tgaaaggttg gtttcgataa tatcgttcca ctcttcatct
    15061 ttcattcgca ttaacaggtt atcacgagtg ataccggcat tattgaccag gatatccact
    15121 tcaccaaatt ctgcgcgaat tttttccaga acagattcga tagatgccgg gtcggtcaca
    15181 ttcaacatca gacctttgcc gttggcacct aaataatcag tgatcgcctg agcgccattt
    15241 tcactggtcg cagtgccgat aactttcgcg ccacgggctg cgagcgtttc agcaattgcg
    15301 cggccaattc cgcggcttgc accggttacc agtgcgattt ttccttcaaa attcatgatt
    15361 ttcctctttt aaagctcgag cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg
    15421 gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca ctttgcccgg gccgacttca
    15481 tagagatgtt ctacgccttg cgctgccatg tattcaacag acttcgtcca ctgaaccggg
    15541 ttatacaact gacgtaccag tgcgtcacgg atggcatcac cattggtttc gcatttcaca
    15601 tcaacgttat tcacaacagg aactgttggt gcgttaaagg tgattttcgc taattctact
    15661 gccagtttgt cggctgctgg tttcatcagc gcacagtgag acggtacgct cactggtaac
    15721 ggcagagcgc gttttgcgcc cgcagcttta caggcagcgc cagcacgctc aactgcttct
    15781 ttatgaccgg caataaccac ctgtcccgga gagttaaagt ttaccggaga aacgacctga
    15841 ccttctgcag cttcttcaca cgctttcgca atagacgcat catccagacc gatgattgcc
    15901 gccatagcac ccgttccttc cggtacggct tcttgcatga acttgccgcg catctcaacc
    15961 agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa ccagcgcgga gtattccccc
    16021 aggctgtgac cagccatcat tgccggtgct ttaccgccct gctgctgcca tacgcgatac
    16081 agcgcaacag atgcagtcaa cagcgctggc tgggtttgcc aggttttatt cagttcttca
    16141 gccggcccct gctgggtcag cgcccacagg tcgtagccca gcgccgcaga agcttcagca
    16201 aacgtttctt cgacaattgg atagctcgcc gccatatcag ccagcattcc aacggtttga
    16261 gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa
    16321 cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg cttcaagcag aaccaactgc
    16381 cccggcttaa tgcgcccgtc gcgtacagct tcatccagag cgcacgggac agaggccgca
    16441 gaggtattac cgtggcgatc cagcgtcacc acgacattgt ccatcgacat gccgagtttt
    16501 ttcgctgttg cactgataat acgcaggtta gcctgatgcg gaaccagcca gtccagttga
    16561 gaacggtcaa gattattcgc cgccagcgtc tcatctacga tgtgcgccag ttctgttacc
    16621 gcaaccttga agacttcgtt gcccgccatc gtcagatgaa ttgaattctc tggattcaca
    16681 cggtcagcat taggcagcgt cagcaactca ccatagctac cgtcggcatg cagatgggtg
    16741 gagatgattc ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa
    16801 ataataatag tcccacgatc ggttggatcg caggtgcgcg ccagtacatc ggaaccgacg
    16861 accagagcat acttcaccgc cccagatttc acgtattgat cggctacgct taatgcatag
    16921 gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc aacctttaat gcccagcatg
    16981 ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg
    17041 atcaggccaa tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca
    17101 aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta
    17161 cgagtgacaa tccactcgtc agaggtgtcc accatttttt ccaaatcggc gtttgtccgc
    17221 acttgttcgg gcagatagct gccagtacca ataatcttcg tatacatgta cgctcagtca
    17281 cttttcggtt atataccgtc acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg
    17341 cagagttccg cttttgccac cgtccagcag ctcaaaacca gctgggtata cagattccag
    17401 gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc
    17461 gaccgcaaaa gctcgctgat tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc
    17521 taacagacag gcgccgttat actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct
    17581 cttttgtagc caacgcttta ataacagtag ccaccacgac cgttttttcc cttcaccctg
    17641 agatttcagc agagaaagga acatcctgac aacaccttcc atcgtcttta atgtgacatt
    17701 tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact cattggcttc
    17761 aagatagccg atataattga tagaagggat tgttttaagc accgctgagg catcccgaat
    17821 actgtcgaga ccctttactt cttcttcacc aatattgagc aacgccacgc gaggattagg
    17881 aatttccacc acctcttcag ccagaactga gcccataatg gcaaattgca ccaacattgt
    17941 actgtcacaa tcgacgttgg cccctaaatc gaggaccacc gttttgccct tttgctgatg
    18001 cggtaatacc gtcaccagcg ccggacgctc aatcccctcc aggggcttga gtaataattt
    18061 tgccagcccc attagcgccc cggtattacc ggcactgaca caggcttgcg ctcgaccttc
    18121 tttcaccagc tccagtgcca cgcgcattga actcccacga ctggcgcgga tagcttgcga
    18181 aggccgggca tcactggcga taactgactg cgcaggaata atctgcagac gcgaacgttg
    18241 ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag
    18301 agtgagttgc gaattagaat tcagtgcctg caatgctgca ggcactgtca cggaagggcc
    18361 aaaatcccct cccatgacat ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc
    18421 cggtttggtc tttccccagt tgcctgggga aaatcctcac taagcttcat cacgcagacg
    18481 cgtgattact tagcgatgac cttgcggccg cggtagtaac cgtcggcagt gatgtggtga
    18541 cgcaggtgtt tttcaccaga agttttgtct acagacaggc tggtgactgc ggtcagcgcg
    18601 tcatgggaac gacgcatgcc acgtttggaa cgggttggtt tattctgttg tacggccatg
    18661 gaccttactc ctcaattacg gtttatcccc gatccaacgg ggagcaccaa ttacttacgc
    18721 tttaagctgg ctaatacggc aaatgggttt ggcttttgcg cttcttcagg cagttcacca
    18781 aagaccatgt ccgcttcgga cacttcacag tgttcagaat catgcaccgg aactaccggc
    18841 aaggcgagga tgatttcatc ttcaaccatt gcaagcagat cgatttcacc gaattcgtta
    18901 acctcaatcg gttcatacgc ttccggcagt gcttcagcct gttcgtctga acgcacagga
    18961 ctaaaacaat acgttgtgta gacctgatga gtaaacggct tcccgcaacg ctgacactcg
    19021 agcgttaccg tcaccttcgc atcgccgttt aacactgcga gacgttggtt atcgatagcg
    19081 aacgacatgg agcattccac atcactgtcc acactgacta cggattcggc gacgcgctca
    19141 acctgatcag gagtatagat accctggtaa tcaaggcgtt tttgagccgt acgaaccgga
    19201 tcgagagtca ggggtaattt taccttttgc atagggcgcg catattaact ttgtaacgtc
    19261 atagagtcaa agaaaaaggc agcctgtggt tgccttttgc caataattcg cacacattgc
    19321 gggttactgc tttattttcg ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg
    19381 catagtttaa aatggctatc atcaatacgc tatatctgat ggaaaaaaat atgcctaaac
    19441 ttattttagc ctccacctcg ccctggcgtc gcgctcttct ggaaaaactg caaatctctt
    19501 ttgaatgtgc agcacccgag gtcgacgaaa ctccacgcag cgacgaatca ccgcgacagt
    19561 tggtgcttcg actggcacaa gaaaaagcgc aatctctggc gtcacgttat ccggatcatt
    19621 taattattgg ttcagaccag gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa
    19681 cggaagaaaa tgcccgtctg caattacgca aagccagcgg caatatcgtc accttctata
    19741 ctggactggc gttgtttaat tcggcgaatg ggcatctaca aacagaagtg gagccttttg
    19801 acgtccattt ccgtcacctg agcgaggcgg agattgataa ttacgtgcgt aaagagcatc
    19861 ccctgcactg cgcgggtagc tttaagagtg aaggatttgg cattacgctg tttgagcgtt
    19921 tagaggggcg agatcctaac acgctggttg gtttaccgct aatcgcgctg tgtcagatgt
    19981 tacgtcggga agggaaaaac ccgctgatgg gatagttttc tgcgccggat gcggttacgt
    20041 cgcatccggc aatcggtgct ggcggcctga tgcggcgtaa acgccttatc aaggccaacc
    20101 gcccattatc aggtttatat gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg
    20161 cttcaaacct tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt
    20221 aaacttcaac gctgcggcgt gcaggaacag acgatttaat cctgtgcctg cttcagtgag
    20281 ctgtctgtca aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacccgcata
    20341 ctgtgtatgc acacggatct gatgagtacg ccctgttacc gggctacaac gcaccagggt
    20401 ggcaaaagca tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact
    20461 cacacgcacg atacgttcgc cgctttgcag aatatttttc agcaacggcg cttgaacgct
    20521 cttcacatgc gactgccact gaccgcgcac cagcgccagg taatcttttt gcatcccttt
    20581 ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac
    20641 acctgaagtg tcccggtcga gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa
    20701 cgcccgcaaa ccttcgataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc
    20761 ggaaggttta ttcagcacca ggatatggtc atcttcatat aagatgacgt ccgccagcgc
    20821 cgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc cgttcagcaa cgcgaaccgg
    20881 tgggatacgc acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt
    20941 cacccgcact tcgcctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa
    21001 ttgggtacgc aaaaagttat cgatacgttg ccccgcttcg tcagcgttga tagcaacaat
    21061 ttttacggat ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc
    21121 gccactcatt tttctatgct tatatttagt tttgcacctt attacttcac tacgtgatca
    21181 ctttattgat ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg
    21241 gtgataaatg gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt
    21301 ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg
    21361 atcatccacg cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg
    21421 gttgcagtcc ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt
    21481 tgataattgc gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga
    21541 gctgacgata accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg
    21601 gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga
    21661 tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg
    21721 ggcagcgtct gtatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca
    21781 tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg
    21841 gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta
    21901 actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca
    21961 ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca
    22021 gtctggcggg tagctatctg gttctgatgc cgaacaaccc gcgcgcgggt ggcatttctc
    22081 gtcgtatcga aggcgacgat cgtaccgaat taaaagaagc actggcaagc cttgaactgc
    22141 cggaaggcat gggacttatc gtgcgcaccg ctggcgtcgg caaatctgct gaggcgctgc
    22201 aatgggattt aagcttccgt ctgaaacact gggaagccat caaaaaagcc gctgaaagcc
    22261 gccctgctcc attcctgatt catcaggaga gcaacgtcat cgttcgcgca ttccgcgatt
    22321 acttacgtca ggatatcggc gaaatcctta tcgataaccc gaaagtgctc gaactggcac
    22381 gccagcatat cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg
    22441 gcgagatccc gctgttcagc cactaccaga ttgagtcaca gattgagtcc gccttccagc
    22501 gtgaagttcg tctgccatct ggtggttcca ttgttatcga cagcaccgaa gcgttaaccg
    22561 ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa accgcgttta
    22621 acaccaacct cgaagctgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg
    22681 gcctgattgt tatcgacttc atcgacatga cgccagtacg ccaccagcgt gcggtagaaa
    22741 accgtctgcg tgaagcggtg cgtcaggacc gggcgcgtat tcaaatcagc catatttctc
    22801 gctttggcct gctggaaatg tcccgtcagc gtctgagccc atcactgggt gaatccagcc
    22861 atcacgtctg cccgcgctgc tccggtaccg gtaccgtgcg tgacaacgaa tcgttgtcgc
    22921 tctctattct gcgtctgatc gaagaagaag cgctgaaaga gaacactcag gaagttcacg
    22981 ccattgttcc tgtgccaatc gcgtcttacc tgctgaatga aaaacgttct gcagtgaatg
    23041 cgattgaaac tcgtcaggac ggcgtgcgct gtgtgattgt gccaaacgat cagatggaaa
    23101 ccccgcacta ccatgtgctg cgcgtgcgta aaggggaaga aactccaacc ttaagctaca
    23161 tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac
    23221 gtaagcgtcc tgaacaacct gcgctggcaa cctttgccat gccggatgtg ccgccagctc
    23281 caaccccagc tgaacctgcc gcgcctgttg tagccccagc acctaaagct gcaacggcaa
    23341 caccagcatc tcctgcacaa ccagggttgt tgagccgctt cttcggcgca ctgaaagcgc
    23401 tgttcagcgg tggtgaagaa accaaaccgg ccgagcaacc agcaccgaaa gcagaagcga
    23461 aaccggaacg tcaacaggat cgtcgcaagc ctcgtcagaa caaccgccgt gaccgtaatg
    23521 agcgccgcga cacccgtagt gaacgtgctg aaggcaacga taatcgcgaa gaaaaccgtc
    23581 gtaatcgtcg ccaggcacag cagcagactg ccgagacgcg tgagagccgt cagcaggctg
    23641 aggtaacgga aaaagcacgt accaccgacg agcagcaagc gccgcgtcgt gaacgtagcc
    23701 gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag
    23761 agcaatctgt tcaggaaacc gaacaggagg aacgtgtacg tccggttcag ccgcgtcgta
    23821 aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag
    23881 tcgcaccggt ggttgaagaa actgtcgctg ccgaaccaat tgttcaggaa gcgccagctc
    23941 cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc
    24001 aagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct
    24061 cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc
    24121 caacccaatc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca
    24181 aagtctggat ccgctatcca attgtacgtc cgcaagatgt acaggtcgaa gagcagcgcg
    24241 aacaggaaga agtacaagtg cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg
    24301 aaccggttgt tagcgcgcca gttgttgaag aagtggctga agttgtagaa gcccccgttc
    24361 aggttgccga accgcaaccg gaagtggctg aaacgacgca tcctgaagta attgccgccg
    24421 cggtaactga acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagttg
    24481 cagaacacgc agaaccggtg gttgaaccgc aggaagagac ggctgatatt gaagaagttg
    24541 ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct gccgcgccag
    24601 tcgtcgctga agtcgcagca gaagttgaaa cggtagccgc ggttgaacca gagatcaccg
    24661 ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg
    24721 aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggc aaaggtgccg
    24781 caggtggtca tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg caacctgttg
    24841 agtaataatt agctcaagta atcaagccct ggtaactgcc agggcttttt tatttcaggg
    24901 gtagcatctt tgctgtaatg acattagcgt tacatgtact gtataattat catccacgca
    24961 aagccgaagg atatatttat gccagcagcc aataacattc cagccaggcg cgaatcttta
    25021 aacttgcgca taaaacccgc agaacgcgat cttattgacc gggccgctaa agcccgagga
    25081 aaaaatcgca ccgaatttgt ccttgaggcc gccagagccg cagctgaaga ggcgctcatt
    25141 gagcagagaa ttttcaggat cgattctgat gattatcagc tatttttgaa tcgtctggat
    25201 cagccccccg cgcccaatgc tgcattacgc aaaaccatgc aaacacccgc gccgtgggag
    25261 caaaaaaaat gattcaggcc cccgagccac tcatggcaag gcatcaattc acctcttttt
    25321 gctcgggagt agaaacgatg gataactggc taaagcagag agccctaaaa aatcagcttg
    25381 ccggcgcatc acgtacattt gttagctgtg atacgtatag caacgttttg gcatattatt
    25441 cgcttgcttc cagcgccgtt gaaacgtatg tcgcgacagg acgatttcgc cgaaatatgc
    25501 cagaaccgat ccctgttgta gtacttggtc gtctggcaat agataaatca ttacagggtc
    25561 agggcatcga tcgtgcgatg gtcagagatg ccggtctacg agtactacag gcggctgaag
    25621 tcattggtat tcgcggaatg ttggtccatg cgttatcaga ccaggcccgt gagttttatc
    25681 tgcgagtggg gtttgaacca tcgcctgtcg attctatgat actgatggca acattggctg
    25741 atttgcagga atgtcttaaa tagttcgtta tgtctcatgc aaaaaaatgc ccgccccggc
    25801 gatcccagag caggcagaca aaacataccc agtttcatga tatgttttaa agcgaaatta
    25861 tttgttgagc tggaagagcg acaatccctg catatcggta aatgctttat acgacgcctg
    25921 caatgccgtt tgctgcatga tgtaagatga aatagttgca ttccagtcca catcaaccag
    25981 atcgctcatc tgctgcgttt gccctaaagc gcgatcgcta cctaatgaat ccagcgactc
    26041 cagttcgttc agctgcgtgc ctaattccgc gcgaacagtc agcacattgt tcagcgagtt
    26101 tttcagtccg cggttggttt tatccagtgc cgcagcggcg gtttctttat ccgcttcgct
    26161 atccgcgacc ggcgttttca gggctgcgat ggcgctatcc agcatggcaa aaagattggt
    26221 ttcagaagcg ctaccgtctg gttccgctac cgcgttgctg gtaatactgt cgaaaatttt
    26281 gtcacccgtg tgccctatca ccatcgaacg cgaagcatcg acctgttgtt taatactttc
    26341 tgctccgcct tcgtattttc catccgcctc gctaaacggt gcggattctg ttttataacc
    26401 ggcaaaaatg tagcgcccgt taccgtcagt ggtgttcgcc agattcagca actggtcacg
    26461 aagcccctga atatccgtag ccagcgaggc ccggtcatcg tcactcaagg tgccattgct
    26521 ggcgtagaca attttttcct gagcattctg gatagcggtg gtgacctggc taagtacact
    26581 ctcttccagt gacacttttt gagtggcgaa agtacgcgcc agcgtgtact ggctgttttg
    26641 cgcctgtgcc tgggagagaa caacagcttg tgatgcagca atgggatcat ctgaagggtt
    26701 aacgactcgc ttacccgtcg acatctgttc gccgtacttc atccattctg cctgagaatt
    26761 ggtgatacca cgcatgtttt gctggtacat catctgggta ctgaaacgca tcttatctcc
    26821 ccttagcgaa tgttaatcag cgcatcaaaa atcgcgtttg ccgtctgcag aacctgcgca
    26881 ttcgccaggt aatactgctg aaaacgttgc agatttccgt actcctcatc gagattgaca
    26941 ccggaaatcg actgctgctg attggaaagc tgcgtcacca cattaccttg cgtggtgcta
    27001 ctggttttca acgtcgcggt tttattaccg atatcactca ctaacgaggc ataagcgtcg
    27061 ttaaaggatt tcgcaccgcc caccgtttta ctgttgcttt gcagatcgag cagggcctga
    27121 ccgttgcggt tgtcgctatc acctgtatct tctgcgctcg ccatggcgat tttcgcttcg
    27181 tcggtgatta atacatccat gttgacgatg gcgtcactta ccggtttcag cgtgaagctg
    27241 tcgttaacgg caggcgttcc tgtaaacgtc aactccagac catcaaatgc cactttaccg
    27301 ctggcatccg gcgtcaccgt aaaagtggta ttactggcaa ggcgggtgac ctgccactga
    27361 ttattatcga acgagatttt gtaatctgtc gccagtaccg cggaggcatc agttaccgtt
    27421 gcaccgatcg caacgtcacc tttgtttttc gtgttttgca gaaccgcggg cttaccgata
    27481 gcaaagaaat cttcaccggc atcgccgtta gcatcaaatc cggctttgtg ttgggtgttg
    27541 aaagcctcgg caaatgccag cgccagttgt ccaagcgtat tacgcgtctg gtccagatcc
    27601 tgagaacgga atgtcagaat gccgcccagc gacccggtat tcagtaattt ctccgggatc
    27661 tcaatattgc ctgccgtccc atcaacataa gcgacagtcg tacgagaagg gtcagcgctg
    27721 gaaggaactg ccgccagttg ccgagccgta cttccctgaa ccagtgagta accattggcc
    27781 atcgtgatgt tataagtgcc accatcctga acgctgactt ctacaccaac aatctggttt
    27841 aattcgctca ccagttgatc gcgttgatcc agcagattgt taggtgacgc ccctgccccc
    27901 acgcctgtca ggcgggagat ttgatcgttc aggctggcga tttgtttagc gtaattgttg
    27961 atctgatcaa cgctggcacc tatcgcgata ttgacctgtt tgtcctggtc gcgcagatat
    28021 tgatcggtgg ttttaaactg attcaccaac ccttctgatt tcccaatcag cgcctggcgc
    28081 gctgccggat cttccgcgtt actcaccagc gtttgcaggc tggtgaagaa atcctgcatc
    28141 tgtgttgcca gcgaagaggt actggtggag agcatattgt cgattttcga catctgctca
    28201 tagcgggcgg tcaggccgct actttgcgtc tgcgcagcac gtaactggtt ggtaataaac
    28261 gcatcatact cacgctgcac accagaaacg tagacgccat tgccaaccca gccgccagcg
    28321 cccaacgtgc tattggcctg cgccataata gtggtttggc gggtatatcc ggcaacgtta
    28381 tagctggaga tattattact tgccgtattt aacgccgcct gggccgcgtt cagtccgctc
    28441 atggcgttat tgatcaagct ggacatggag gttccttata agccttcaat tacagagtat
    28501 tatcggcagc gccccgccgg acttgagtta ttcagaacag attatcaatg ttcatgctgt
    28561 aggttttgct caccttgtcg cttatcgatt tcatctgctg aatcatgctg gtgagtttgc
    28621 gggcatagtg aggatcggtg gcatagcccg cgtcctgtag ggcctgcgcc ccctgctccg
    28681 cactcgcggc ggtcgtcacg gcggcgtagc gcgggttacg cgttaacagc ccaacgtaat
    28741 ccgacaatgc ttccagatac gagctgtaga cccgaaactt cgcttttact ttcttcgctt
    28801 cgccattttc atattcagtc gtggtgattt cagtgactgg ccctttccag ttgccagagg
    28861 ctttgacgcc aaacaggtta tagctcggct cgccgttttc acggcggatt tgccgttgtc
    28921 cccagccaga ttccagcgcc gcctgagcga ggatcaaatg atgtggcaca ccgctttgct
    28981 ggcttgccag ttgggcgggc aacgagagtt gcgcgaggaa tgctttactg tcacccggca
    29041 gcgaatcatc gtagttacgt ggtacggcct tttgcaccag ctgcgaaagc gtctgatttt
    29101 gataacgcac cacggtttcg agcgggaatt tcatcggtgc tgctggcgtg gactcctctg
    29161 gcaatggttg ttctggcgtc atctgtttaa ccatcatctc tgccagcccc agacctttgc
    29221 ccgccgtcat ctgttgggca atctgctggt catacatact ggtatacagg cgagtgtgct
    29281 cgctgctgaa caggccatct tttggtaaag cgtcgcgcat gcttttcaac atcatctgca
    29341 cgaacatccc ttccacctgg cgggccaccg gacggatatt tgccgccgga tcttcgcccg
    29401 ctttcgcctt aagttcgttg agcgattgtg catcccaggc cgcacttgcc agtagtttgc
    29461 tgtcgctgat catcagatga tttccagttt tgcccgcaga catcccgcac tttgcattga
    29521 ttgcaaaata gacatcagat ccatcggcgt agcgcccagc gcattgagcg cacgcaccac
    29581 gttattgagg ctggcgctgg aacgtacgct ttgcagcgaa ccgccgctct ggcgtaaatc
    29641 gatctgcgtt tgtggagtaa ccacagtctg tccaccacca aacggtgtat ctggctggct
    29701 gacattggcc tggcgattaa ctgttactga gagatttccc tgcgctaccg cgcagctgtc
    29761 gagggtcact tcgcgattca tcaccaccga accggtgcgc gagttaatca ctactttagc
    29821 gtcctgcggg gtaacattaa cctgcatatt ctggatatcg gcaaggaaac ggacctggga
    29881 actgttgcca ctcggtacgc gcacctgaat agtccgcgca tccaacgcgg tggcgctgcc
    29941 atatccacgc actcggttga tggtgtcagc gatttgctgt gccatgctga aatcttcgtc
    30001 gttaagttgc aaattaaggg tattcccgac gccaaactgg ctgggcaatt cacgttcaat
    30061 aaccgcacca ttggtgatcc gtccaccgtt cagttggttc acctgaacac tgctaccgcc
    30121 agcggaggcc cctgcgccgc caaccagaat attgccctgc gccagcgcat acacctgact
    30181 gtcaacgccc ttaagcggtg tcatcaataa cgtaccgcca cgcagacttt tggcatttcc
    30241 catggaagaa accaccacgt caatggtttg cccctgacgt ccaaacggtg gaagtgacgc
    30301 tgtcaccata accgcagcga cgttttttag ctgcatatta gtgcccgtcg gaacggtaat
    30361 tcccagctgt gagagcatgt tattaagcgt ttgtgtggta aacggcgtct gagttgtctg
    30421 gtcaccagtt ccatccagcc ccaccaccag gccatagcca atcagtgagt tttgccttac
    30481 cccctgaaca ctggtgagat cgcgaatacg ctcagcctga gccgccgtcg taaccagtag
    30541 aagaattaat gcagagagaa atttaatcac cacagcctca cttacattgg agacaggtta
    30601 agaaagaaac gctgcaacca gcccatattt tgcgcttcgt taatgtagcc attgcctacg
    30661 tattcaatgc gcgcatccgc cacctgagta gacggtacgg tattgctgcc gctgatagtg
    30721 cgtggattaa ccacgccaga gaagcgaata aattcggtac cctgattaat ggcgatctgt
    30781 ttttcaccca ccacatgcag gttgccgtta accagtacct ggtcaaccgt caccgtcaac
    30841 gtgccgctaa aggtattact ggcattggcc ccgccttttc cgttgaacgt gttaccaccg
    30901 gaggcttcga catcggcacg agcgttacca aacagcccct gcaaatagcg cggcacagta
    30961 tcaaagccaa aattagtttt accgtcacgg ctggcattcg cagaggagct tttgctggcg
    31021 ctgacgttct cctgcaacac gatggtcagc gtatcgccaa tattgcgtgg tcgacgatct
    31081 tcaaacagcg gttgatagcc atagttaatc ggctgagcag actggaaaat agaaccgttg
    31141 gcgacgggcg tcggaccggg aaccggttgt gcactggtcg ccccctgtac cagcggcgtg
    31201 gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca agctggaaat ggcataagta
    31261 tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc agtaaaccgg tgaatctgcc
    31321 accggttatg ccttagagtt gcgtcagttt ttgcagcatc tgatcggtgg tggataccgc
    31381 tttactgttg atttcgtaag cgcgttgcac ctgaatcata ttgaccagtt cttccgccac
    31441 gttgacgtta gacgtttcaa catacccttg atacagcagt cccgcgccgt tcaggcccgg
    31501 cgtgctttcg ttcggtgcac cggaggattg cgtttcggtg tagaggtttt cgccaatact
    31561 ctccagtccg gtatcattca taaaggtggt gagattgagc tgcccaacct gaaccggagc
    31621 tgcctggcct tgttgtgtta cgctgaccac gccatcacga ccgatggtga tacttagcgc
    31681 attcgccgga atggtgatcg ctggctgcac ctgaaaacca cctgccgtca ccagctgccc
    31741 gttctgatcc acctggaaag agccgtcacg ggtataggct gatgagccat ctggcaacat
    31801 cacctggaaa aagccctgcc ctttaatcgc gacatctttg ctgttgttgg tctgcgacag
    31861 attgccctgg ctgtgtaagc gttcagtggc gaccgggcgt acacccgtgc cgatttgtaa
    31921 tccggatggt aaggtggttt gttcggaaga ctgagcacct ggctggcgaa tggtttgata
    31981 aagcagatct tcaaataccg cgcgctgacg cttaaaaccg ttggtactga cgtttgccag
    32041 gttgttggca atgacgtcca tattggtttg ctgggcatcc aggcccgttt tggcgatcca
    32101 taatgaactt atcataaaat gtcctgtagt ggattagtga caaagtgcat ctgtcttctt
    32161 gccggatgcg gcgtaaacgc cttatccggc ctacaagagc tcgcgaaatc aataaattgc
    32221 gattgcccca tagacccgat aaacttgcgc atcgggcaac ttatacttgt catgtatcct
    32281 ttcaattaac tcatcgacag cagttggttg gctcggcctg cgttatcatc gacgctgctg
    32341 atcaccttca tctgcatttc aaaacgccgc gcgctggcaa tcatgtcgct cattgccgca
    32401 acggcattga cgttactgcc ttccagaaca cccgacatca cacgcaaggt tggatctgcc
    32461 tgcagtaccg gcccacgcgt ggtctgggtt tctgcgctta aacgaaaaat gccgtcgtca
    32521 ccgcgctgga cttcgctgcc cgtggctttc accagtttca gacgccccac tggcgcaacc
    32581 gtatttgccg gatcgcccgg attcagcgcc gagattgtgc catcggcagc aatagtgatt
    32641 tccgcccctt ccggcacagc aattggccca gcctcgccta tcaccggatg tccctgaatt
    32701 gtcagttgcc cggtgggatc aacctgaatg ctgccattac gcgtataccc ttcgctaccg
    32761 tcagcggtct gcacggccag ccagccatcc tgctgcaacg caacgtccag cgggcgcgag
    32821 gtgtaatcca tcttgccggg cgtcatgtct gcacccggcg ttgacgccgt taccagcgtg
    32881 cgcgtaggca gtgaaagccc ttccaccggc actgcgcgca aggcgtttaa ctgcgcgcga
    32941 aaaccgggcg ttgaggcatt ggccagatta ctggcggtta ccgcctgttg attcaacgtc
    33001 tggctggctg ctcccatcgc ggtataaatt gcgtgatcca ttgagctatc ccgtcagcga
    33061 ttagcgtaag ttaaccagcg tgttgaggat ctggtcctgg gttttgatgg tctgggcgtt
    33121 agactgatag ttacgctgag caacgatcat attgaccagt tctttactga gatcgacgtt
    33181 ggacgcttcc agcgcaccgt tggtcagggt gccaaagttg cccgtcccgg ctgtccccaa
    33241 cagcgccacg ccagaagatt gcgtcgcaga ccagacgttg tcgccttcgg atgccagacc
    33301 ttcgttgttg gcaaagttcg ccagtacaat ctgccccagc agttgggttt gttcgttgga
    33361 atagttgccg acaaccgtac cgtcatcatt gatttgataa ctcaccagat cgcccggttt
    33421 gtagccgttc tgggtggttg ccacaatgtt gttagcgccg gtattttgct gcatggagtt
    33481 gaggaagctc aggctaaacg tggcgggttc tgcaccgtta attgcgccag tggaaatatt
    33541 cgctgttgga tctgaggtca gaacgccatt ggcattaaac accagcgtca ttgcaggctc
    33601 agctgtacct gttggatcac tgctatcctg ggtgtagacc tgccagttat tatccccggt
    33661 cttcacaaag tagacgctca tgtcatgagc attaccctga ctatcgaaaa cagtcaccga
    33721 accttttttg ttatagctat ccgcattgct agcatcaaat gcgttaacag agggaagcgg
    33781 atcactggaa ttcaggttga tctgcatcga cgccgtggtg gtagttttcg ctgccatcag
    33841 ggtattcgga atcgaaatat tggtcggatt cgccccttgc tgaatagtcg gcggcgtacc
    33901 ggttgccggg taacccgtca gctgtaaacc ttgcatattc accaggttac ggttttcatc
    33961 cagcttaaat tgtccgttac ggctgtagaa caccgaaccg ttgctgtcta ccagacggaa
    34021 aaaaccgttc tggctgatag caacgtccag acctcgcccg gtgttggtgg tcgtgccatc
    34081 ggtaaagtcc tgagtgatac cagcaacttt tacccccagt cccactttcg aaccggcaaa
    34141 catatcggca aaagaggccg tgcctgattt aaagccgtag gtggcagagt tggcgatatt
    34201 gttgccaata acatcgaggt tggtggcggc agcgtttaat ccgctaaccg cttgagaaaa
    34261 ggccatgact gactcctgaa atgtgaaggc ttaaattatc tgccgtactt cgtcgagggt
    34321 ggtggtgccg taagtgccga gatccagcgt attaccgttg ttgccgcgga tcacaccctg
    34381 caccagagca aactgcagcg gctgggcaac cagttgtgta ccaccgttac tggcgctaat
    34441 cgctacgttg taagaaccgt tcggcgcagt ggtgccgtca gtcaacgtac cgtcccaggt
    34501 gaaactgtgg actccggcgg tcagttcacc aatatcaatg gtacggacaa ccgcaccgtt
    34561 tttatcggtg atggtggccg tcactttgtc tgccgcctgt tgcagctcaa caccaaacgg
    34621 cgtggtcgtg gtcacagccc cttcttcact gccggttccc gcaagaacag tggtgccggg
    34681 gatcatcacg ccgtgaccga tcaggttact ggcctgtaac gactggctgt tatcaatctg
    34741 tccggaaata gatccgagcg tggtattgag tttttcaatc ccgctgaccg tgctgatttg
    34801 agccaattgc gacgtcagct cgttgttttc cattggattg gtcgggtcct ggtttttcag
    34861 ctgcgccacc aacaaagtca gaaaactgct ttgtaaatct gcggcgttgc tgcccgtgag
    34921 cgaactacta ctggtggtgc tgacgccggt atttgtcgga tcggtggtgg ttaccgcaat
    34981 ggacatagtt ttctccttta ttgaccgagc gtaagggttt tcagcatcat gcttttcacc
    35041 gtgttgagca cttcaacatt ggcctgatag ctgcgtgacg ccgacatggt gttaaccatc
    35101 tctccgacga catcaacgtt tggcattttc acgtagccct ttgcatccgc cagcggatta
    35161 cccggttcat aaaccagttt gtccggggcc tgactttcta taacatcggc aacctttacg
    35221 ccgcctgtcg cagcacctgg cgcagcgtta acctgaaata ccacctgttt tgcccgatat
    35281 ggctgtccat cgggaccggt cacgctatca gcattcgcca gattactggc cgccacgttc
    35341 aggcgctggg actgagcagt taacgccgac ccggcgatat caaaaatatt cagcagtgcc
    35401 atccgttaat ttccgctctg taaaacgttc atcatgcctt tgatttgccc gctcaacgcg
    35461 ctaaggctca tctggtattg caggctgtta tcggcaaact gggtgcgttc gcgatccata
    35521 tcgacagtat taccatccag cgagggctgg tccggaatac ggtattgcag ttctgcggta
    35581 ggaggcgtca gcgcctgcgc cggaatgtgt tgcgttgagg tcatcgtcag tgcaaccacg
    35641 cttgttgcat cccgtccacg ttgcatgact tttttaagtt cactggcaaa atcgatatcg
    35701 cgcgcctgat aaccaggggt atcggcattg gcgatgtttg ctgccagcac ttcctgacgc
    35761 tgggcgcgca gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag cttatcgagc
    35821 atatctcctc cgcaggtatc aaaattcggc catcagctta aatgccttta cgcgcgcgtt
    35881 atcggcggaa taaacgcaaa atgggtcgct atttatgccg ttgatggtca ttgcggacag
    35941 gtacaattca cgttgtagaa atggctgggg ggcgaaaatg ctggcaataa aacgtagcgt
    36001 ggcgatcatc gcgatactgt tcagtccgtt aagtgcggcg agcaatctca catcgcaatt
    36061 gcacaccttt tttagcgccc agctcgcggg ggtaagtgat gaggttcgtg tttctattcg
    36121 tacagcgccc aatctactac cgccgtgcga gcagccattg ctttcgatga gcaataattc
    36181 ccgcctgtgg ggcaatgtga atgtgttggc acgctgcggt aacgacaaac gatatttaca
    36241 ggttaatgtg caggccacag gaaattatgt ggttgccgca atgcccattg tgcggggagg
    36301 aaagctggaa gctggcaatg tcaaactgaa acgcggacgg ctggacacct tgccaccgcg
    36361 tacggtgctg gatatcaatc aacttgttga tgccgttagc ctgcgcgatc tatcacccga
    36421 tcaacctatc cagttaaccc agtttcgcca ggcatggcgg gtaaaagcgg gacaacgcgt
    36481 caacgtgatc gccagcggtg atgggtttag cgccaacgca gaaggtcagg cgctgaacaa
    36541 tgctgccgtc gcacagaatg cgcgggtgcg catggtatcg ggacaagtgg tcagcggcgt
    36601 tgttgatgca gatgggaata ttcttataaa cctgtaagct gttaaagatt acccgtccct
    36661 tgccgataaa taagcaacac atgataaaag cgccctcaat gaggaataaa ccatgagtat
    36721 tgatcgcact tcgcctctga agcccgtaag caccgttcaa ccgcgcgaaa ccactgacgc
    36781 gccggtaacg aacacccggg cggcaaaaac aaccgcctcc accagcacca gtgtgacgtt
    36841 aagcgacgcg caagcaaaac tgatgcaacc cggcagcagt gatatcaatc ttgaacgtgt
    36901 cgaagcgtta aaactagcga ttcgtaacgg tgaactaaaa atggacaccg gcaaaattgc
    36961 cgatgcgctg atcaacgaag cgcagcaaga cttgcagagt aactgatcgt atgacgcgtc
    37021 ttgcagagat cctcgaccag atgtccgctg tgcttaacga tctcaaaacg gtaatggatc
    37081 aagagcagca acatctctct atggggcaga tcaacggcag ccagttgcaa tggattacag
    37141 aacaaaaaag ctctctgctg gcgacgctgg attacctcga acagttacgc aggaaagaac
    37201 ccaatgcagc aaatagcgtt gatattagtc aacgctggca ggaaattact gtgaaaacgc
    37261 agcaactacg ccaaatgaat caacataacg gctggttact ggaaggacag attgagcgca
    37321 atcaacaggc gctggaaatg ttgaaaccgc atcaggaacc gacgctatat ggagcgaacg
    37381 gtcagacctc aacaacccat cgcggcagta aaaagatttc gatctgaaga ttatctccgg
    37441 cctgcactgc aggccggaat gcctttttac accgtccggc gggcaaactc tttaactttg
    37501 aagcccagta ccgccagtgc tgcaaagtac gcggcaatcc ccgccagcac gaccgccatt
    37561 aaacgcagta aacgccaggg catggtaccc aatgaccact ccggcatgat atgtaacata
    37621 cctaaaagca cgccagacat caccagtacc gccaccacca gacgcaacag aaacgccatc
    37681 cagccgggtt gcggggtaaa gattttctgc ttacgcaact gccagtaaag cagcgaagca
    37741 ttcagacacg ccgccagacc aatagaaagt gacagcccgg catgtttcaa cggaccgata
    37801 aacgccaggt tcatcaattg cgttaaaatc agcgtaacga tggcaatttt cactggcgtt
    37861 ttaatgtcct ggcgggaata aaagccagga gccaacactt tcactacaat caggccaatc
    37921 aaacccaccg agtaggcaat taacgcccgc tgggtcatca gcgcatcaaa ggcggtaaat
    37981 ttaccgtact ggaacagcga aacggttaac ggacctgaaa gaatgcccaa cgcaaccgca
    38041 ctcggcagcg ccaacaggaa acaaagacgc agcccccagt ccatcaaacg gttgtattca
    38101 tcatgattgc cactggcaaa acttttcgac agcgacggca gcaaaatggt gccaagcgcc
    38161 acgcccagta caccggacgg aaactccatt aagcggtcgg cgtaatacat ccaagacacc
    38221 gaaccggaag caagaaacga ggcaaaaatg gtgttgatga ttaaggagat ctggctcaca
    38281 gagacgccaa ggatcgccgg tcccatctgt ttcaccacgc gcattgctcc ggcatcgtgg
    38341 aagttaatgc gcggcaggac cagcatgccg atcttcttca ggtgcggtag ctgatacacc
    38401 agctgcagga tgccgccgac cgtaacagcc cacgccagcg ccagcaccgg tgggttaaag
    38461 tacggtgcgg caaacagcgc gaaaccaatc atgctgatat taagcagtgt tggagcaaac
    38521 gccggaatcg agaagcggtt ccacgtattc agaatcgctc ccaccagcga cgccagggag
    38581 atcagcaaga tatagggaaa ggttatcttt agtagctggc tggtcagggc aaatttgtcg
    38641 gcagtgtcag cgaagcctgg cgcggtcacc atgatcaccc acggtgcagc gagcatgcca
    38701 gcgaccgtca caaccgccag cgcgagtgtc agcaggccag aaacataaga gacaaagacc
    38761 cgcgtggcgt cttcaccctg cttgctttta tattctgcca gaatcggtac aaatgcctgg
    38821 gaaaatgccc cttcggcaaa gatacggcgc aacaagttag gaagtttaaa agcgacgaaa
    38881 aaggcgtcgg ttgccatccc tgcgccaaag attctggcga caattgcgtc acgtgcgaag
    38941 ccaagcacac gcgaaaacat ggtcatcgag ctgacggcgg ccagcgattt taataaattc
    39001 atcggtgttc taatccagac ccacgggcaa aacgcctgca aagcaggcgc tgaaagaggc
    39061 gaatagtcta cctggattat ggtgaattgc taccgccaga tgttacaggg tttattcact
    39121 catcgcatcg cgccagatct tgtcaacgat acgttgcgcc cgcactgcct gttcgccggc
    39181 ggtttgcgga actgtctgat tttgcacgca ttcaatgaag tgccgcgcac agccgacaaa
    39241 tccacgttgc tcaagcgtac tctgccaacc aggaatcggt ttatgcacta cgccctgccc
    39301 gcgctcctca cgccattcgc gcatatccgt aatgtcgatg agcgcaccgt cagtcacggc
    39361 ctgcacggtt tcacgctggc ttccggcacg gcgatgcata caggtggtga tctgcaaagg
    39421 accggccgaa aaatggtgct cggcaaacag catttcgcca gcgtcgttag tcagtagcgt
    39481 accgccatcc agagaggctt tgccgcccga caaccacagc gcggtatcca ccacatgcag
    39541 ataatcatcc agcaacgtga aataaagatc gtgtggcccg acgctattgc tacgatgttt
    39601 atccattctt agcgaggctg cggtggcgag ttgcgttttt aactctccgt agagtggtgc
    39661 gaaacgacgg ttaaaaccga ccatcaacgt cagttttttc cgcgccgcca gttccaccaa
    39721 ccgttcagca tcgcgcagat tttctgccag cggtttatcg acacagacat gtacccccgc
    39781 attgagtaac gtactgacca cgtcaaagtg gctggcggtg ctggaatgca caaaaaccgc
    39841 atcgcaactg gcggcaaggc tggataacga atcggcataa ggaatgcgcc agctttcaca
    39901 aattggcagg gctttcgcgc gcgtaggcga ccaggctcct tgtaacatcc agtcagacgc
    39961 tgccgccagc accggtaacc acgctttttg cgcaatgccg cctaatccca ctacgccgat
    40021 acgtaatttt ttcactttaa tctcccagat gggcaagcaa cgaatcaaga cgctgtttca
    40081 gttctgccac ttcgatttcc agggcttcga cgcgagcctg taaatcaccg tcaaccgcgt
    40141 tcgacatatc catcaccgcc ggctgatctt caacctcacc actgaaaaga tgcatgtagc
    40201 ggctttcacg tttacccggt tcgcgggcca gacgcaccac aaaaggacca tcttcgcgat
    40261 ttgccagttg ttccagcgtc gactccactt ccgccatatc gctgaattca tacattcgcg
    40321 cggcgcggct gcgcagttca cccggcgtct gggcaccacg caataacaac gtggtgatta
    40381 acgccacttc tgcggcgctc agtttcagat cgccaaattc tgaattacaa aaacgttgct
    40441 catatttggt gacccgatta ccaaaaccgc tcactgtgcg tagataatga cgtttgacca
    40501 gattatccag ctgttcctgc acttcggatt cactcagatt catgaccggt tcacggttcg
    40561 ttttctgatt acaggccgtg actacaccat tgactgagag cgggtattgc tccggcgtgg
    40621 tcacctgctt ttccagcaag caaccaatca cgcgagcttc aagggcagta agttgatatt
    40681 tcatgagatt ctccttagcg gccgggcgtc cagtctgggg tagttaatgc cgtcagtacg
    40741 tgatcgcgcc attgtccatc aatcaacaga tagtctttcg catagccttc tttttcaaaa
    40801 cccagtcgcg ccagtaaatc accgctgcgt ttattgtgcg gcatataatt agccataatg
    40861 cgatgaatat gttgggtgcg ctgcatataa cgaatggctg cggtcagggc ttcaaacatg
    40921 agtcctttgc cctgccattt ttgcccaatc gaataaccga gatagcaggc atgaaaagag
    40981 ccacgaacaa cattggaaaa attggcaacg ccaataatct ctttttcgtc cgggtcgaat
    41041 aagccaaagt agaaagctga accttgttta tgaaattcgt taatcatccc tagcctggcc
    41101 tgccagcctg atggataaca gtggctttcg tcgcgcactg gctcccaggg cttgaggaaa
    41161 tggcgattct ctgcgtaata atccgccaga cgccaggcat cacgatcatg caccagacgc
    41221 acgaccagtc ggtctgtggt taagcgcact tttggcacgt tactgcgata gccaaacatt
    41281 ctatacctac tccttcccgt aacgtcttta atacgctgta agtattacta tacctgcgca
    41341 accagaccct gtgaaaagtc cagaatgcca ttttctgcat tattcacata tttgataaaa
    41401 actctctatc aaagcgacgt tttgataaaa aaatattgtc cccgaagtga tgggaaaagt
    41461 cttagcggca atgcagaata gaaagtgctc atcttttatt tcccgggagg ggaaatgtcc
    41521 cgcgtgtcgc aggccaggaa cctgggtaaa tatttcctgc tcatcgataa tatgctggtc
    41581 gtgctggggt tctttgttgt cttcccgctg atctctatcc gcttcgttga tcaaatgggc
    41641 tgggccgccg tcatggtcgg tattgccctc ggcctacgcc agtttattca gcaaggtctg
    41701 ggtattttcg gcggagcaat tgccgaccgc tttggtgcca aaccgatgat tgttaccggt
    41761 atgctgatgc gtgccgccgg attcgccaca atgggtatcg cccacgaacc gtggctactg
    41821 tggttttcat gcctgctttc gggactcggc ggcacgttgt ttgatcctcc gcgttcggcg
    41881 ctggtggtga aattaatccg tccacagcag cgtggtcgtt ttttctcgct gttgatgatg
    41941 caggacagtg ccggtgcggt cattggcgca ttgttgggga gctggctgtt gcaatacgac
    42001 tttcgcctgg tctgcgccac aggggcagtt ttgtttgtgc tgtgtgcagc gtttaatgcg
    42061 tggttgttac cggcatggaa actctccacc gtacgcacgc ccgttcgcga aggcatgacc
    42121 cgcgtgatgc gcgacaagcg ttttgtcacc tatgtactga cgctggcggg ttactacatg
    42181 ctggctgtac aagtgatgct gatgctgcca attatggtca acgacgtggc gggcgcgccc
    42241 tctgccgtta aatggatgta tgccattgaa gcgtgtctgt cgttaacgtt gctctaccct
    42301 atcgcccgct ggagtgaaaa gcattttcgt ctggaacacc ggttgatggc tgggctattg
    42361 ataatgtcat taagcatgat gccggtgggc atggtcagcg gtctgcaaca acttttcacc
    42421 ctgatttgtc tgttttatat cgggtcaatc attgccgagc ctgcgcgtga aaccttaagt
    42481 gcttcgctgg cggacgcaag agctcgcggc agctatatgg ggtttagccg tctgggtctg
    42541 gcgattggcg gcgctattgg ttacatcggt ggcggctggc tgtttgacct gggcaaatcg
    42601 gcgcaccagc cagagcttcc gtggatgatg ctgggcatta ttggcatctt cactttcctt
    42661 gcgctgggtt ggcagtttag ccagaaacgc gccgcgcgtc gtttgctgga acgcgacgcc
    42721 tgatttgata ctcccccctg actcctccat actgaaagtg aactgacagc caaatggagg
    42781 agtcacgtga agctatacat ttacgatcac tgcccttact gcctcaaagc ccgcatgatt
    42841 ttcggcctga aaaatatccc catcgaatta catgttctgc tcaacgacga cgcagaaaca
    42901 cccacccgga tggtcggtca aaaacaggtt cccattctgc aaaaagatga cagccgctat
    42961 atgccagaaa gcatggacat cgttcactat gtcgataaac tcgacggcaa accgttactg
    43021 accggcaaac gttcccctgc cattgaagag tggctgcgca aggtcaatgg ctacgccaac
    43081 aaactgctgt tgccgcgttt tgccaaatcg gcatttgatg agttttctac tcctgccgcg
    43141 cgcaaatatt tcgtcgacaa gaaagaggct agcgcgggta attttgccga cctgctggcc
    43201 cactctgacg gtctgattaa gaatatcagc gatgatttac gtgcgctgga caaactgatc
    43261 gtcaaaccga acgccgtgaa tggcgaactt tcggaagatg atattcagct atttccgctc
    43321 ctgcgtaatc tgacgctggt agccggaatc aactggccaa gccgcgttgc tgattaccgc
    43381 gataatatgg cgaaacagac acaaatcaat ttgttatcat caatggcgat ttaatcctga
    43441 cccgccgcgg cagcctgtat tcgcgggttg ccgccgtttt cagttttttc tgctggcgcg
    43501 tttatcgctg gtgacacatg ctgcgttgtg tcaggatatt gacgcagatt tcatgtcgag
    43561 gactaccatg aacaagtttt tatttgccgc tgcattgatc gtcagtggcc tgctcgttgg
    43621 ctgtaatcaa ctcacccaat acaccatcac cgaacaagaa attaaccagt cgcttgcgaa
    43681 acataataat ttctcaaaag atatcggttt acccggcgtg gctgacgccc atattgttct
    43741 gacaaacctg accagccaaa ttggtcgcga agagccgaat aaggttaccc taaccggaga
    43801 cgccaatctg gacatgaact ccctgttcgg tagccagaaa gcgaccatga aactgaagct
    43861 gaaagcgctg cccgtgtttg ataaagaaaa aggcgctatc ttcctgaaag agatggaagt
    43921 ggtcgatgcg acggtacaac cggaaaaaat gcaaacggtg atgcaaacgt tgcttcccta
    43981 tttaaaccag gcattacgca attactttaa ccagcaacct gcttacgtcc tgcgcgaaga
    44041 tggcagccag ggcgaagcaa tggcgaagaa actggcgaaa ggcattgaag tgaagccagg
    44101 cgaaattgtc attccgttta ctgattaatc acgagggcgc attcgcgccc tttatttttc
    44161 gtgcaaagga aaacgtttcc gcttatcctt tgtgtccggc aaaaacatcc cttcagccgg
    44221 agcatagaga ttaatgactg caccatccca ggtattaaag atccgccgcc cagacgactg
    44281 gcaccttcac ctccgcgatg gcgacatgtt aaaaactgtc gtgccgtata ccagcgaaat
    44341 ttatggacgg gctatcgtaa tgcccaatct ggctccgccc gtgaccaccg ttgaggctgc
    44401 cgtggcttat cgccagcgta ttcttgacgc cgtacctgcc gggcacgatt tcaccccgct
    44461 gatgacctgt tatttgacag attcactaga ccctaatgag cttgagcgtg gatttaacga
    44521 aggcgtgttc accgctgcca aactttaccc ggcaaacgca accactaact ccagtcacgg
    44581 cgtgacgtca gttgacgcaa tcatgccggt acttgagcgc atggaaaaaa tcggtatgcc
    44641 gttactggtg catggtgaag tgacacatgc agatatcgac atttttgatc gtgaagcgcg
    44701 ctttattgaa agcgtgatgg aaccgctacg ccagcgtctg actgcgctga aagtcgtttt
    44761 tgagcacatc accaccaaag acgctgccga ctatgtccgt gacggaaatg aacggctggc
    44821 tgccaccatc actccgcagc atctgatgtt taaccgcaac catatgctgg ttggtggcgt
    44881 gcgtccgcac ctgtattgtc tacccatcct caaacgcaat atccaccaac aggcattgcg
    44941 tgaactggtc gccagcggtt ttaatcgcgt attcctcggt acggattctg cgccgcatgc
    45001 acgtcatcgc aaagagagca gctgcggctg cgcgggctgc ttcaacgccc caaccgcgct
    45061 gggcagttac gctaccgtct ttgaagagat gaatgctctg cagcactttg aagcattctg
    45121 ttctgtaaac ggcccacagt tctatggctt gccggtcaac gacacattca tcgaactggt
    45181 ccgcaaagag caacaggttg ctgaaagcat cgcattgact gatgacacgc tggtgccatt
    45241 cctcgccggg gaaacggtac gctggtccgt taaacaataa aaatcgtagc ttcctgttgt
    45301 cattaggtta ttttaactgt ataaataacc agtatattca acagggggct attatgcgaa
    45361 ttgaagtcac catagcgaaa acttctccat tgccagctgg ggctattgac gccctggctg
    45421 gcgaactttc ccgccgtatt cagtatgcgt ttcctgataa tgaaggccac gtatcggtac
    45481 gttatgccgc agcgaataat ttatcggtta ttggcgcgac aaaagaagat aaacagcgca
    45541 ttagcgaaat tctccaggaa acgtgggaaa gcgccgatga ctggtttgtc agcgaataat
    45601 atgcagtgat ttttgttttt gccgggtcgc cccggctttt ttattttact tgcgaattgt
    45661 gagtacaaaa tttctaca
//
LOCUS       DAZUCC010000014        42329 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZUCC010000014 DAZUCC010000000
VERSION     DAZUCC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.014"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            262..888
                     /locus_tag="ACHY09_000672"
     CDS             262..888
                     /locus_tag="ACHY09_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFS3883688.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTHRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            909..3011
                     /locus_tag="ACHY09_000673"
     CDS             909..3011
                     /locus_tag="ACHY09_000673"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFS3883689.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRKTLQR
                     LGLEDIAADLAAHNLLPA"
     gene            3024..3662
                     /locus_tag="ACHY09_000674"
     CDS             3024..3662
                     /locus_tag="ACHY09_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFS3883690.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDHADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3726..4394
                     /locus_tag="ACHY09_000675"
     CDS             3726..4394
                     /locus_tag="ACHY09_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFS3883691.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTMQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4391..5176
                     /gene="phsC"
                     /locus_tag="ACHY09_000676"
     CDS             4391..5176
                     /gene="phsC"
                     /locus_tag="ACHY09_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFS3883692.1"
                     /translation="MNPSQHAEQFQSQLANYVPLFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSANDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     TVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5180..5992
                     /gene="ydhT"
                     /locus_tag="ACHY09_000677"
     CDS             5180..5992
                     /gene="ydhT"
                     /locus_tag="ACHY09_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFS3883693.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5998..7608)
                     /locus_tag="ACHY09_000678"
     CDS             complement(5998..7608)
                     /locus_tag="ACHY09_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFS3883694.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEEIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAIAQAVVKPASRARRRLSFNQM"
     gene            complement(7734..8039)
                     /locus_tag="ACHY09_000679"
     CDS             complement(7734..8039)
                     /locus_tag="ACHY09_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFS3883695.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTNEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8147..8223)
                     /locus_tag="ACHY09_000680"
     tRNA            complement(8147..8223)
                     /locus_tag="ACHY09_000680"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8187..8189),aa:Val,seq:gac)
     gene            complement(8228..8304)
                     /locus_tag="ACHY09_000681"
     tRNA            complement(8228..8304)
                     /locus_tag="ACHY09_000681"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8268..8270),aa:Val,seq:gac)
     gene            8613..9869
                     /locus_tag="ACHY09_000682"
     CDS             8613..9869
                     /locus_tag="ACHY09_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFS3883696.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSTYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGSINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9910..11283)
                     /gene="mdtK"
                     /locus_tag="ACHY09_000683"
     CDS             complement(9910..11283)
                     /gene="mdtK"
                     /locus_tag="ACHY09_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFS3883697.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            11498..12139
                     /gene="ribE"
                     /locus_tag="ACHY09_000684"
     CDS             11498..12139
                     /gene="ribE"
                     /locus_tag="ACHY09_000684"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFS3883698.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12179..13327)
                     /gene="cfa"
                     /locus_tag="ACHY09_000685"
     CDS             complement(12179..13327)
                     /gene="cfa"
                     /locus_tag="ACHY09_000685"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFS3883699.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13618..14829)
                     /gene="punC"
                     /locus_tag="ACHY09_000686"
     CDS             complement(13618..14829)
                     /gene="punC"
                     /locus_tag="ACHY09_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFS3883700.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            14942..15874
                     /gene="punR"
                     /locus_tag="ACHY09_000687"
     CDS             14942..15874
                     /gene="punR"
                     /locus_tag="ACHY09_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFS3883701.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15871..16896)
                     /gene="purR"
                     /locus_tag="ACHY09_000688"
     CDS             complement(15871..16896)
                     /gene="purR"
                     /locus_tag="ACHY09_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFS3883702.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17195..17284
                     /gene="cydH"
                     /locus_tag="ACHY09_000689"
                     /gene_synonym="ynhF"
     CDS             17195..17284
                     /gene="cydH"
                     /locus_tag="ACHY09_000689"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFS3883703.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVVVGLTAALH"
     gene            17450..18619
                     /locus_tag="ACHY09_000690"
     CDS             17450..18619
                     /locus_tag="ACHY09_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3883704.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18765..19346)
                     /gene="sodB"
                     /locus_tag="ACHY09_000691"
     CDS             complement(18765..19346)
                     /gene="sodB"
                     /locus_tag="ACHY09_000691"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFS3883705.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19474..20301)
                     /gene="mepH"
                     /locus_tag="ACHY09_000692"
     CDS             complement(19474..20301)
                     /gene="mepH"
                     /locus_tag="ACHY09_000692"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFS3883706.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASANFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20636..20983
                     /gene="grxD"
                     /locus_tag="ACHY09_000693"
     CDS             20636..20983
                     /gene="grxD"
                     /locus_tag="ACHY09_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFS3883707.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21034..25650)
                     /locus_tag="ACHY09_000694"
     CDS             complement(21034..25650)
                     /locus_tag="ACHY09_000694"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS3883708.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSMERLDTLLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQADEWYSRVRRAAPWKDLPRRVFDVTLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAKLGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARTLLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEANQANSSALQQAIVTVLADGGAWFAQQISQRIRDKIGESVDPSAMQEAL
                     WALVWQGVITSDIWAPLRALTRSSTNTRTSTRRSHRARRGRPVYAQPVSPLVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANAWGNL
                     LPWPAHPATLIPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEIFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25743..26390)
                     /gene="rnt"
                     /locus_tag="ACHY09_000695"
     CDS             complement(25743..26390)
                     /gene="rnt"
                     /locus_tag="ACHY09_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFS3883709.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26493..26900)
                     /gene="gloA"
                     /locus_tag="ACHY09_000696"
     CDS             complement(26493..26900)
                     /gene="gloA"
                     /locus_tag="ACHY09_000696"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS3883710.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26981..28078)
                     /gene="nemA"
                     /locus_tag="ACHY09_000697"
     CDS             complement(26981..28078)
                     /gene="nemA"
                     /locus_tag="ACHY09_000697"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFS3883711.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGKPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28115..28714)
                     /gene="nemR"
                     /locus_tag="ACHY09_000698"
     CDS             complement(28115..28714)
                     /gene="nemR"
                     /locus_tag="ACHY09_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFS3883712.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATPAV"
     gene            28817..29056
                     /locus_tag="ACHY09_000699"
     CDS             28817..29056
                     /locus_tag="ACHY09_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFS3883713.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29105..30001
                     /locus_tag="ACHY09_000700"
     CDS             29105..30001
                     /locus_tag="ACHY09_000700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS3883714.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDYLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30082..30603
                     /gene="sodC"
                     /locus_tag="ACHY09_000701"
     CDS             30082..30603
                     /gene="sodC"
                     /locus_tag="ACHY09_000701"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFS3883715.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30604..32616)
                     /locus_tag="ACHY09_000702"
     CDS             complement(30604..32616)
                     /locus_tag="ACHY09_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS3883716.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELQHL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGSNALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32616..33473)
                     /locus_tag="ACHY09_000703"
     CDS             complement(32616..33473)
                     /locus_tag="ACHY09_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFS3883717.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSNLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33476..33712)
                     /locus_tag="ACHY09_000704"
     CDS             complement(33476..33712)
                     /locus_tag="ACHY09_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFS3883718.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLSLAILIAW"
     gene            33913..34347
                     /gene="slyA"
                     /locus_tag="ACHY09_000705"
     CDS             33913..34347
                     /gene="slyA"
                     /locus_tag="ACHY09_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFS3883719.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(34394..34861)
                     /gene="slyB"
                     /locus_tag="ACHY09_000706"
     CDS             complement(34394..34861)
                     /gene="slyB"
                     /locus_tag="ACHY09_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFS3883720.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35135..36244
                     /gene="anmK"
                     /locus_tag="ACHY09_000707"
     CDS             35135..36244
                     /gene="anmK"
                     /locus_tag="ACHY09_000707"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFS3883721.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLILSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36342..36671
                     /gene="mliC"
                     /locus_tag="ACHY09_000708"
     CDS             36342..36671
                     /gene="mliC"
                     /locus_tag="ACHY09_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFS3883722.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLFERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36730..37386
                     /gene="pdxH"
                     /locus_tag="ACHY09_000709"
     CDS             36730..37386
                     /gene="pdxH"
                     /locus_tag="ACHY09_000709"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFS3883723.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37515..38789
                     /gene="tyrS"
                     /locus_tag="ACHY09_000710"
     CDS             37515..38789
                     /gene="tyrS"
                     /locus_tag="ACHY09_000710"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS3883724.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38848..39711
                     /gene="pdxY"
                     /locus_tag="ACHY09_000711"
     CDS             38848..39711
                     /gene="pdxY"
                     /locus_tag="ACHY09_000711"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFS3883725.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEDVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39755..40360)
                     /gene="gstA"
                     /locus_tag="ACHY09_000712"
     CDS             complement(39755..40360)
                     /gene="gstA"
                     /locus_tag="ACHY09_000712"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFS3883726.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40466..41968)
                     /gene="dtpA"
                     /locus_tag="ACHY09_000713"
     CDS             complement(40466..41968)
                     /gene="dtpA"
                     /locus_tag="ACHY09_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFS3883727.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT        10652 a        10387 c        11071 g        10219 t
ORIGIN      
        1 ataataaata tcagttcact tataatttct tgctttgtga ttttaattga taacgatcaa
       61 tgttaagagc ccagaataat cacattattt tttctcctaa ccataaagga ttagtttatc
      121 agcgttaaat cttttctcat acactattgg gttagggaaa atatctggtg ttgaggacag
      181 agtcagcagc gtattagttg agtgcctaat atcaggctgc tgaattgtca gcactgtgtt
      241 tgcgaagagg atgcgaaata aatgaacccg gttgatcgtc cactattaaa tattggctta
      301 acgcgattag aattcttgcg tatatcagga aaaggccttg ctggtttaac aattgcccct
      361 gcgttactgt cacttttggg ttgcaaacag gaagatattg atagcggcac agtaggcttg
      421 ataaatacac ccaaaggggt gctggtgacc catcgtgcgc gatgcacggg ttgccaccgc
      481 tgtgaaatct cttgtaccaa cttcaacgat ggctcagtag ggacattctt ctcccgtatc
      541 aaaatccatc gcaattattt ctttggcgat aacggggttg gctctggtgg cggcctgtat
      601 ggcgatctca actataccgc ggacacctgc cgtcaatgca aagagccgca atgcatgaac
      661 gtctgcccga ttggtgcgat tacctggcag cagaaagaag gctgtattac cgtcgatcat
      721 aaacgttgta ttggctgtag cgcctgtact acggcatgcc cgtggatgat ggccaccgta
      781 aataccgaaa gtaaaaaatc ctcgaaatgt gtgttatgcg gtgaatgcgc aaacgcctgc
      841 ccaacagggg cgttaaaaat tatcgagtgg aaagatatta ctgtgtgaat cttgcaaagg
      901 aaaaaatcat ggctaacggt tggacaggta atatattaag agtcaatctc acgacaggaa
      961 atattaccct cgaagattcc agtaagttta aaagttttgt cggtggcatg ggcttcggct
     1021 acaaaattat gtatgacgaa gtaccgccag gcacgaaacc tttcgatgaa gcgaataaat
     1081 tagtttttgc taccggccca ttaactggat ctggtgcccc ctgtagttct cgcgtaaata
     1141 tcacctcact ttctactttt accaaaggaa atttagtcgt cgatgcccat atggggggct
     1201 tttttgcagc gcaaatgaaa ttcgctggat acgacgtcat tattatcgaa gggaaagcga
     1261 aatcaccggt atggctgaag attaaagatg acaaagttag ccttgaaaaa gccgatttct
     1321 tatggggaaa agggacgcgc gcaacgacgg aagaaatttg tcgcttgacc agtccggaaa
     1381 cctgtgtggc ggctattggt caggctgggg aaaaccttgt tcctctctct ggcatgttga
     1441 atagccgtaa ccacagcggt ggtgcgggaa ctggcgcaat aatgggttcg aaaaacctga
     1501 aagcgattgc ggttgaaggg acgaaagggg tcaacattgc cgatcgtcag gagatgaagc
     1561 gtctcaacga ttacatgatg actgaactta tcggtgcgaa taacaaccat gtcgtgccaa
     1621 gtacgccgca atcgtgggca gagtattcag atcccaagtc acgttggact gcacgtaaag
     1681 ggttgttttg gggcgcggct gaaggtggtc cgattgaaac gggtgagatt ccgccaggca
     1741 atcagaacac ggtcggcttt cgtacctata aatccgtttt tgacttaggt ccggcggcag
     1801 agaaatacac agtaaaaatg agtggctgcc actcttgccc gatccgttgt atgacccaaa
     1861 tgaatattcc tcgggtgaaa gagtttggcg tgcccagcac aggtggtaac acttgtgtag
     1921 caaactttgt ccataccacc atcttcccga acggtccgaa agattttgaa gataaagatg
     1981 atggtcgtgt gattggtaac ctggttggtc tgaatctgtt cgatgactac ggcctatggt
     2041 gtaactatgg tcagcttcat cgcgacttta cctattgtta cagcaaaggg gtgttcaagc
     2101 gtgttctgcc tgctgaagag tatgcggaaa ttcgctggga tcaactggaa gcgggtgacg
     2161 ttaacttcat taaagatttt tactaccgtc tggcgcatcg cgtgggtgag ctgagtcacc
     2221 tggctgatgg ttcatatgcc atcgcagaac gctggaattt gggtgaagag tactggggct
     2281 acgcgaaaaa taaactctgg tcgccgtttg gctatccagt tcaccacgcc aatgaagcgt
     2341 cagcgcaggt cggctccatt gttaactgta tgttcaaccg tgactgcatg acgcataccc
     2401 atatcaactt tattggttcc ggcttgccat tgaaactgca acgtgaagtg gcgaaagaac
     2461 tttttggttc tgaagatgct tacgatgaaa ccaaaaacta cacgccaatc aatgacgcaa
     2521 aaattaaata tgctaagtgg tcgctgttgc gggtctgttt gcataacgcc gtcaccctgt
     2581 gcaactgggt ctggccaatg accgtttcgc cgctgaaaag ccgtaattat cggggcgatc
     2641 tggcgcttga agccaaattc ttcaaagcga tcactggcga agatatgact caggaaaaat
     2701 tagatttagc tgcagagcgt atctttacgt tgcatcgcgc ctacacggta aaactgatgc
     2761 aaactaaaga tatgcgtaat gaacacgatc ttatctgttc ctgggtattc gacaaggatc
     2821 cgcagatccc ggtctttact gaaggtacag acaaaatgga ccgtgacgat atgcatgctt
     2881 cgctcacgat gttctacaaa gaaatgggct gggacccaca gcttggttgt ccgacccgca
     2941 aaacattgca gcgtctgggg ctggaagata ttgccgccga tctggcagca cacaatctac
     3001 tgcctgcgta aggaatgggt gagatgaacc atcaggatga attgccgtta gcgaaagtca
     3061 gcgaagttga tgaggcgaag cggcaatggt tgcaggggat gcgtcacccc gttgatacgg
     3121 taaccgaacc agaacctgct gaaatcctgg cagagttcat tcgtcagcat tctgcggctg
     3181 ggcagctggt ggcgcgcgcc gtgtttctct cgccgcccta ttcggttgca gaagaggaac
     3241 tgtcagtttt gctggaaaac attaagcaga acggagacca cgcggatatc gcgtgtatga
     3301 cgggaagcca ggatgattac tattactcaa cgcaggccat gagtgaaaac tacgcggcga
     3361 tgtctttgca agttgtggag caggacattt gtcgcgccat tgcacacgcc gttcgttttg
     3421 agtgccagac gtatcctcgt ccttacaaag tcgccatgct gatgcaagcg ccatattact
     3481 tccaggaagc gcagatcgaa gccgcgatcg ccgcgatgga tattgcacct gaatatgctg
     3541 atatacggca ggtggagtca tcgacggcgg tgttgtactt gtttagcgag cgatttatga
     3601 cctatggcaa ggcgtatggc ttatgcgaat ggttcgaggt agagcagttt cagaacccct
     3661 gaaatttcag ggagatgtca tggatagggt ggacagtagc agccactgca ttaggggata
     3721 atcagatgtc cttcactcga cgcaaatttg ttctggggat gggaacagta atctttttta
     3781 ctggttctgc gtcttctctg ttagcgaaca cgatgcaaga aaagaaagtt cggtatgcca
     3841 tgattcatga cgagtcacgg tgcaatggct gtaatatttg cgcccgcgca tgccgtaaaa
     3901 ctaaccacgt ccccgctcag ggaagccgtt tatcgatagc acacattcca gtcaccgata
     3961 acgacaacga aacgcagtat cacttttttc gccagtcatg ccagcactgt gaagatgctc
     4021 cttgcatcga cgtttgcccg acaggggcgt cgtggcgtga tgagcagggg atcgtgcggg
     4081 tagaaaaatc gcagtgcatc ggctgcagtt attgcattgg cgcttgtcct taccaggttc
     4141 gctaccttaa ccctgtcact aaagtggcag ataaatgtga tttctgcgct gagtcccgac
     4201 tggcaaaagg ctttccgccc atttgcgtca gcgcctgtcc ggaacacgca ttgatctttg
     4261 gtcgcgaaga tagcccagaa attcaggcgt ggttgcagga aaataaatac tatcaatatc
     4321 agctacctgg tgccgggaaa ccgcacctgt atcgccggtt cggtcaacat ttgattaaaa
     4381 aggaaaatgt atgaacccgt cgcaacatgc tgaacagttt cagagccagt tggcgaacta
     4441 tgtgccactg ttcactcccc aattttggcc ggtgtggttg atcattgccg gactattgct
     4501 ggtagggatg tggttggtgc tggggctgca tgccttgctt cgtgctcgtg gcgtgaagaa
     4561 atcagccaac gaccatggtg agaaggttta tctttacagc aaagcggtca gattatggca
     4621 ctggtcgaat gcgttactct ttgtattgtt gctggcaagt gggctgataa atcactttgc
     4681 gctggtgggc gcaactacgg ttaaaagtct ggttgcggtg catgaagttt gcggattttt
     4741 gttactggca tgctggctcg gctttgttct gatcaatgcc gttgggggta atggtcacca
     4801 ctatcgcatt cgtcgtcagg ggtggctgga gcgagcggca aaacaaacgc gattctattt
     4861 gtttggcatt atgcaggggg aagaacatcc tttcccggca acaacccagt ctaaatttaa
     4921 tcccttacag caggtcgcct atgttggtgt catgtatgga ttgctgccat tgttactatt
     4981 gacggggctg ctgtgtctct atccgcaagc cgtgggagat atgtttcctg gcgtaagata
     5041 ctggttattg caggcgcatt ttgctctggc atttataagc ctctttttta tcttcggtca
     5101 tctttatctt tgcaccacgg ggcgtacgcc acacgaaacc tttaaaagca tggtcgatgg
     5161 ctatcaccgg cactaacgaa tgatcattac acgagcagac ctgcgagaat ggcgtatcgg
     5221 agcggtgatg tatcgctggt ttctgcgcca tttcccacgc ggcggtagtt atgccgatat
     5281 tcatcatgca ctgattgagg aaggatatac ggactgggcg gagagtctgg ttgagtatgc
     5341 gtggaaaaaa tggctggcgg atgaaaactt cgcgcatcag gaagtttctt ctatgcaaaa
     5401 actggccaca gatcccggag agagagcatt ttgcagtcag tttgcccgca gcgacgatca
     5461 tgctcgcatc ggctgttgtg aggataatgc cagaatcgca accgctgggt atgccgcgca
     5521 aattgccagt atgggttata gcgtgcggat tggaagtgtc ggctttaaca gtcacattgg
     5581 cagctccggt gaacgcgcac gggttgctgt aaccggtaat tcttcgcgga taagcagcgc
     5641 tggagatagc agtcggattg cgaataccgg aatgcgagta cgcgtttgta cattgggtga
     5701 acgatgtcat gtcgccagta atggtgacct ggtgcaaatc gccagctttg gcgcaaacgc
     5761 cagaattgcc aacagcggcg acaatgtaca catcattgcc agcggtgaag attcaacaat
     5821 agtgagcacg ggcgtggtcg actcaattat tctcgggcca gggggaagcg ccgcactggc
     5881 atatcatgac ggcgaaagag ttcgcttcgc cgtcgccatt gaaggtgaaa acaatattcg
     5941 agccggagtc cgctatcgcc ttaatgaaca gcatcagttt gttgagtgct gatagcttta
     6001 catttgatta aacgaaagac gccgacgagc acgggatgca ggctttacga ccgcctgagc
     6061 tatcgcctca ccaatttctg cacatgccgt aagtccctgt acgaatggct ggtcgtgcat
     6121 caaccagggc aacgcgccga acgctacgcg cccacgaatt tcttcgggtt gctgtagcgt
     6181 ataatcttcg ccaacatcag ggatttcatc ccctgttttc tgtaattgtt cgcgtagtcc
     6241 agggaaagga atatctttca ctttaagcgg acgctgccca cgggcatcaa taaaaacgtc
     6301 aaacgagtag ctattgtctt ccgttttcag gacggtgcgc gactcattaa tttccatttc
     6361 gtagtcttca ccgagggcga gaatatgaat gattcccgct tcacgtaagg ccagtagtcg
     6421 acgaatagac tctgaaggga ttgccgcata attatcgatg aaaacccggg caaggccttt
     6481 actgaaccgt ttatggtcgt gttcattcag atgaggaaca atttcctgta cggcttcatg
     6541 caggcgcaga atgacataac gccagggaac agtatgtttt tctcgtttat tgcgttcgac
     6601 ttcctggaga tttttttctg cccagtcgaa tgggtcgcgt tgtttgcgct cggcaaacca
     6661 ggcttgagca aaggaatcga cattcaggct ttctaaggct atgcgttggc tccagtctgg
     6721 atcagcaaat ttgatttcct ctactatcaa tctaaatacc cgatccaaaa ggcccacttc
     6781 gcctttttga atctcagcat ttaatgcttg atcggtgacg atgtgtaagg gctcgtaggg
     6841 aatagggcaa tagaaatcgg cttcgggtaa aatacccgta cgcgacatca acgtaatatt
     6901 tagcttttca cttgcgttat cgcggtgaaa aacgacgtgt tgtttatcat cttcaatgaa
     6961 cgaaccatgc tgaatagcca ctgccattgc cgcatccagt ccgctcaagg atgttcccat
     7021 aatacccacg ttacacgcat cgacctttgc ttccatcagg cctgaccacg ggctgggaaa
     7081 atacgttcgg gttgcttctt cttcatcagg ccagacgtga cccgtggcga tcaccgctaa
     7141 atcaaacgtc tcgctgggta aatcctgatt tgtagcgagc atgacgccag catttgtaat
     7201 ttgcagatcg gtaacctggc atgattcata aacagccact gcaaattttt gctgtcgtgc
     7261 ctggtctact aatcgtaaaa attgatcgcg gaaatattcg cccagcagaa ttcgcggtaa
     7321 aaactgacga tcgtgcaagg tttctttttt aacgccataa cgctggagat ggctggcttc
     7381 ttgcttttgt agccattcga gatacgtaca ataaatcggc ggtatttcaa tactggcaat
     7441 atttgccagc atcatttttg agttttcctc atcactgtat ggcatcccga caccggcttc
     7501 atcagcctgc tcgaagatag aaatagaaag tggagtttgt tgctgtagaa gcgagaataa
     7561 ggtgtagatc cccgtaggcc cggcacccac aatagcaatt tttttcattg ttacttacct
     7621 gtacgcattc tttcgaattt agtgttatca gataagtttg gttgagattt ttagaatttg
     7681 agaaatttca gacagggatg aaaagggcgc taaccgccct tgattaaatt ctgtcaggcg
     7741 agttttgcct gattgatttg actaagtggc tcattgacat cgaaaacttt ggcgacaact
     7801 tcctcaacgc cgagattttt cagtcgggca gtatgttttt ccagataggc aagggcgctt
     7861 ttttcattag taaaaaggta gatcccaccg gcttcgtggt tcttttcact ttctgtccat
     7921 accttccaca gaaaaccagg ttcctgatta atcgactcag caagcggttt aagctgctca
     7981 gccattgcgt cgccaaatgg gccattgaaa gaaaaatgaa gttgtaacaa agttgccata
     8041 gcgattctcc ttctgataac gcatacgaag acagtgtaaa tcttttaacg tattggatat
     8101 agagaagaaa cagagggaaa aaggagaaga ccagattaag aaaatctggt gcgtccgagt
     8161 ggactcgaac caccgacccc caccatgtca aggtggtgct ctaaccaact gagctacgga
     8221 cgcaggatgg tgcgttcaat tggactcgaa ccaacgaccc ccaccatgtc aaggtggtgc
     8281 tctaaccaac tgagctatga acgcaacgtt gtaggtgaca acggggacga atattagcgg
     8341 cagagtggga aggtggcaag aggcaaaacg taattttctg cgctatttcg accgtttgca
     8401 gagcttttaa gcaaattggc tatattttgt tgatttgcaa gggcgatttt tattcaggat
     8461 cgcatttaca tcagatacaa cccaataatc cgtgtatcag agtttaaaca gagttgtttg
     8521 ccaccatctc aaaatctgtc gtggataaat cctccgtagc ctttacactt aggtagcaat
     8581 aaaggcacac agatgcagca aaggaataaa tcatgggatc tgatgcgaaa aacttgatga
     8641 gcgacgggaa cgtgcaaatt gttaagaccg gcgaggtcat tggcgcgacg caacttactg
     8701 aaggtgagtt aattgttgaa gctggcggaa gagccgaaaa taccgtggtc acgggggctg
     8761 gctggttgaa agtggcaacc ggtgggatcg ccaaatgcac acagtacggc aacaatggca
     8821 cgctatcggt cagcgacggt gccattgcca cagatattgt tcagtccgag ggaggcgcaa
     8881 ttagtctctc tacgctcgct acggttaatg gccgccatcc cgaaggtgaa ttcagcgttg
     8941 ataaaggtta tgcctgcggt ttgttgctgg aaaatggcgg taacctgcgt gtactggaag
     9001 gccatcgcgc ggaaaaaatt attctcgatc aagagggtgg cctgttggtc aatgggacaa
     9061 cctcagcggt cgtggtagat gaaggtggtg aattgttggt gtatccaggt ggggaagcca
     9121 gcaattgtga gattaatcag ggcggcgttt ttatgctggc ggggaaagcc agtgatacat
     9181 tgcttgctgg tggcaccatg aataatctcg gtggtgaaga ctctgacact attgttgaga
     9241 atggatccac ttatcgtctg gggacggatg gtcttcagct ctacagttcc ggtaagacgc
     9301 aaaacctgtc cgttaatgtg ggtggtcggg ctgaagtgca tgccggtacg ctggaaaatg
     9361 cggtaataca aggtgggaca gtcatcctgt tgtcacccac cagcgcggac gaaaattttg
     9421 tcgtagagga agatcgcgca ccggttgaac tgaccggtag tgttgcatta ctggacggcg
     9481 cttcaatgat tattggctat ggcgcagatc tgcaacaatc aacgattact gtacagcagg
     9541 gcggtgtatt gattctcgac ggcagtacgg taaaaggtga cagtgtcact ttcagtgttg
     9601 gtagcatcaa tctaaatggc ggaaaactgt ggctaatcac tggtgcggca acgcatgtgc
     9661 aactgaaagt gaaacgcctg cgcggagagg gagcgatttg cctgcaaacc agtgcgaaag
     9721 aaatttcacc tgacttcatc aatgtgaaag gggaagttac tggtgatata cacgttgaga
     9781 taacagatgc cagtcggcaa actctgtgta acgcactgaa actacagcca gacgaagacg
     9841 ggattggcgc aacgctccag cctgcgtaaa aacacaggcg caacccgtga aggttgcgcc
     9901 ttgtctttac tagcgagctg ctcgttgcag aatgatggct gacggcagac gttgcaggaa
     9961 ccgcatacgc agcatcatca taatggctgc cgacgtcagg ccaataataa agcctatcca
    10021 gaagcctgct ggccccatag gttcaacgac cagatcggtc agtgccagaa tatagccgct
    10081 tggcaagccc agcacccagt aagccgtaaa ggtaatatag aaaatggaac gcgtatcttt
    10141 atatccacgc aaaatcccac tgccaatcac ctggattgag tcagaaatct gatataccgc
    10201 cgccagcaac atcaaatgcg cagccagcgt tacaacctcg ggattgtcgt tgtacaacag
    10261 ggcgatttgc tcccgcagtg aaaccgtgaa aatggccgtc agggttgcca tacagacacc
    10321 caccataagc ccggtccgcg cagcggtttg cgcatccagc gttgagccct gacccagacg
    10381 ataacctacg cggatggtta ccgctgccgc cagcgacatg ggaagtacga acatcagtga
    10441 actaaagttc agggcaatct gatgtcctgc gacatcaaca ataccgagcg gagacactaa
    10501 cagagccacg acggcaaaca gcgttacttc aaagaacagc gccagcgcaa ttggcaaacc
    10561 gagttgaatc agtcgtttca taaccgcagg atcgggcttc gcagtgcctt tttcgttacg
    10621 aatatcgcgc atcgagcggg cgcgtttaat gtaagaaacc atggcaagga acatgaccca
    10681 atacaccgca gcagtagcca cgccacaacc aacgccaccg agctcaggca taccgaaatg
    10741 accgtaaata aagatatagt tcaccggaat gttcaccagc aggccgataa agcccatcac
    10801 catacccggc ttggtttttg ccagaccttc acactggtta cgggcaacct ggaagaaaag
    10861 atagcccggc gcgccccata gcaacgcacg cagataaccc acggctttgt ccgccagagc
    10921 cggatcgatg ttttccatgg agcggataat gtaacctgca ttccacagca ccagcataat
    10981 gagaacggaa acaaaacccg ccagccagaa accttgtcgc acctgatgcg caatgcgctc
    11041 acgtcgaccg gaaccattta attgcgcgat aaccggcgtt aatgccagca gcagtccgtg
    11101 accaaagagg atcgccggaa gccagataga agtaccgata gcgaccgccg ccatgtcggt
    11161 ggcactatag ccgcccgcca tcacggtatc gacaaaaccc atcgcagttt gggcgatttg
    11221 cgcgagaatc accgggattg ctaatgctaa taacagacgc gcttcactga tatacttctg
    11281 cacgtgaaca ccttttattt gtagttatat gagagactaa aaagccgcct gaatgggcag
    11341 caaaaagaag aagcagggga aattcagtct attgtagcga ggatttatta attctccagt
    11401 gaaaaaatag tcattcttac cccttcactg gtaagggcgt ttgccacctg ctattgtgct
    11461 gaacagaatg tcttaactga tttcaggagt tgtaagtatg tttacgggga ttgtacaggg
    11521 caccgcaaaa ctggtgtcga ttgacgagaa accaaatttt cgcacacatg tggtggagtt
    11581 acccgaccac atgctggacg gtctggaaac cggtgcttcc gtggcgcata acggttgctg
    11641 cctgaccgtg acggaaatta acggcaacca tgtcagtttt gacctgatga aagaaacgtt
    11701 acgcattacc aatcttggcg atttaaaagt gggggattgg gtaaacgttg agcgtgcggc
    11761 gaaattcagt gatgaaattg gcggacactt aatgtcaggt catattatga ccaccgctga
    11821 agtggcgaaa atattaacct cggaaaataa tcgccagatc tggtttaaag tccaggatag
    11881 tcagttgatg aaatatattc tgtacaaagg atttattggc atcgacggta ttagcctgac
    11941 cgtcggtgaa gtcacgccaa cgcgtttttg cgtccattta attccggaaa cactggaacg
    12001 cacgactctt gggaagaaaa aacttggcgc acgcgtcaac attgaaatcg atccacaaac
    12061 tcaggcagtg gtagatacgg tagaacgagt gctggcggca cgagaaaatg ccatgaatca
    12121 accaggtaca gaagcctgat ctgaaatcgc ccccggagag ggggcgatag aatagccttt
    12181 agcgagccac tcgaaggccg ttttcaacac cgcgtgagaa cacgacctgc cagagctgaa
    12241 tatcacgggc gcggaaagca cccgcacagg cattcagata ataggtaaac atccgtttaa
    12301 agcgttcact gtagttatcc gcaatttctg gccatgcggc gaggaatcgt tcataccacg
    12361 ccatcaacgt agtatcgtaa tcagcaccga agttatgcca gtcttccatc acaaagtggg
    12421 gttcgctgga ctgagcaatc tggcgtacag agggcaggca accgttcgga aaaatatatt
    12481 tattaatcca gggatcaaca ttcagatcgg tttttttcga accgatagta tggagcagga
    12541 atatgccttc cggtttcaaa ttacgatcca ccaccgcaaa ataggtatcg taatttttcg
    12601 gtccgacgtg ctcgaacatc cccacagaaa caatacgatc aaactggtcg ttcaggtcac
    12661 gataatcttg cagcaaaatg gtgacatcca ggccttcaca gcgttcctga gccatttttt
    12721 gctgttcggc agaaatggtg acgccgacca cgcttacgtc ataattagac gccatgtagt
    12781 gcgccagtcc accccagcca cagccaatat ccagtacgcg catccctggt tttaactgca
    12841 atttttcaca aatcattttg agcttcgcct gctgggctga ttccagatta tcggcatctt
    12901 tccagtaagc acaggaatat tgcatgaagg gatcaagcat gcggctgaac aagtcattac
    12961 ccaaatcgta atgctctttg ccgactatcc aggcacgttt tttactctgc agattgaaga
    13021 gacgagcgcc ggcaatacgc agcgtgtctt tgaaatgatg ggggagttgg ttctcgagac
    13081 ctgcgcgtaa gactttgcta aaaaacatat ccagtcggtc acattcccac cagccatcca
    13141 tataactttc gcctaacccc aacgatcctt cctgcagaac gcgtttaaaa aaatcggggt
    13201 ttttcacacg aatatccgcc ggggcagaac cgttaatggc tataccggca cggctaagta
    13261 attcgttggc gatacggtac cagttgtcat ccggtacact gacttcttct atacacgatg
    13321 aactcatagt ttctccatca ctggtcggtg atcagaacct taaaacagcg tagacgcttt
    13381 ttgggctttg tgagaaatct cgcagaaaaa accgcgagtc tataatccga cgtagaacag
    13441 agggaaagga ggaacccttg ccgaaatgac catccatagt gagtcgggaa cgatcctgtc
    13501 cccgtaaaaa cataatattt atcgtagctt taacaactcg aaattagtat aggccgctaa
    13561 tttcaatcat acaacaaaat tttgttagca gcctaagtat aatcatatcg atatagatca
    13621 gtgtgattcg ctatgagcga cttcggcatt gccatgattc tgacagtcta cttcttcaca
    13681 acgttgcatc atgtaaccca gagcgaccag cactactgtt gataacatca cgctggtggt
    13741 ggtgagcaat ggcgtgctga tactgatcag ccaggaaact accagacttg cgaggaagca
    13801 cagacccagt tgtagagtgt tctgcaacgc tgcggcgcga ccggttgcat gtgggaaggg
    13861 acgcagcgcc tgggcgacaa caatcgggta gatcgcgcca ttggcaatcg ccatcacaca
    13921 gaatgggatc aggatttcga ctagcgacac atggctaatg aagcccgcag cccaggtcgc
    13981 aatgacgctg acagcaaaca gtaccagcaa ccacggtaat aactgcttgc cttgccattt
    14041 ctgcagtgcg gcgcgacagc cataaccacc aatcagaaac gcgatagttt gcgggacata
    14101 acttaaacca ataactgccg ggctgtagcc catttcacta aggatgaacg gtgagccggt
    14161 cagccatgca aaaaaactgg ctgaacaggc cgcatatatc agcacgttgc cgcgataggt
    14221 tttagaacgt agcaggtcgg taaaggtcag accatcctga ctattgttac gggccttcgt
    14281 cgtgggtttg agccagaaaa taggcagaat cagcaccacg gtaatggcaa acagggtggc
    14341 gaaaatcgcc tgccaggaaa aatggaccag cagccagctt cctaacagag gagccagtgc
    14401 cggagataga cccaccagcg gcatgatggt cgcaaaaata cggttaactt tctgtgaagg
    14461 ataataatcc gtcactaacg cttgccagat aactgccgcg gcgcagacac ccacagcctg
    14521 tacaaaacgc aataccagca gcgtagcggc gttttctacc cacagcatcc ccagactacc
    14581 taacgcaaaa attgtcaggc cgattaataa taccggttta cgaccataac ggtcggagag
    14641 cggcccccac agaagctggg ctgcggcaaa accggcaagg aacagactaa ggctggcact
    14701 gacagcagac gcaggcgttt gcaggtcggc ctgtatggcg gcgaaagcag gcagatacat
    14761 atcggttgcc agaaaaccga gtacgctcaa acccgccagc cagactaaaa atcttttccc
    14821 aggttgcatg tatattctct aaatctctca attaaacacc gccgcaaagt gtagggagtg
    14881 aaattagtgt tgtgaaacgg taatatttga tgactggttt caaaaatttt gcaggcagac
    14941 tatgtggtca gaatattcac tcgaagttgt tgatgcggta gcgcgtaatg gtagttttag
    15001 cgctgcggca caggagttgc atcgcgttcc ttctgcggtc agctataccg tgcgtcagct
    15061 ggaagagtgg ctggcggtgc cgctgtttga acgacgtcac cgtgatgtgg aactgaccgc
    15121 tgccggggcg tggtttctca aagaagggcg ctctgttgtc aaaaaaatgc agatcacccg
    15181 gcagcaatgt cagcagatag cgaacggctg gcgcggtcag ttagctatcg cagtggataa
    15241 tattgtcagg ccagaacgta cacggcagat gatcgttgat ttttatcgcc attttgatga
    15301 tgtcgaactt cttgtctttc aggaagtgtt caacggtgtc tgggatgcgc tttccgacgg
    15361 gcgcgtggaa ctggcgattg gcgcaacacg cgcgattccg gtaggcggtc gttatgcctt
    15421 ccgcgatatg gggatgctaa gctggagttg cgttgttgcc agccaccacc cgctggcgtt
    15481 gatggatggc ccgttcagcg atgatacgtt gcgcaactgg ccgtcgttgg tgcgcgaaga
    15541 cacctcgcga acgctaccta aacgtattac ctggttgctg gataaccaaa aaagacttgt
    15601 cgtgcctgac tgggaatcat cggcaacctg tatctcggca ggattatgca tagggatggt
    15661 gccaacacat ttcgccaaac cgtggcttaa tgaggggaag tgggtagcac tagaactgga
    15721 aaaccccttc ccggattcgg catgttgtct gacatggcag caaaatgata tgtcgcctgc
    15781 gttaacctgg ttgcttgagt atctcggcga tagtgagacg ctgaataagg agtggctgcg
    15841 ggagccggaa gagactcccg caacgggtga ttaacgacga tagtcgcgga acgggccgtc
    15901 agccacggag cggcgttcaa tcaagcgcgg atgcacttca atggactgcg gttcttcgcg
    15961 tttattgacg atacgatcca acagcatgtt gaacgccgtt tcgcccagcg aatcttttgg
    16021 ctgatggatc gtggtcagcg ctggcgtaaa atagcgggcg ttgcgcacgt tatcataacc
    16081 gatcagcgaa acatcctgcg gcacgcgcag acccatttca tcagcagcac aaagtgcgcc
    16141 cattgccatg atatcgccac cacagaagac ggcagtaggg cgatgcggtt gcgacagtat
    16201 ttgctgcatg gcgcgataac cggattcagg ttcaaagtca ccctgtacaa tccagctttc
    16261 cggcactttg atcatcgctt cttccatcgc cttcataaaa ccggcaaggc ggcctgcgcc
    16321 ggtgttacgt tccagcgggc cagggataac gccgatttcg cgatgaccgc gttcaatcag
    16381 ataacgcccg gccatgtagc cgccttcgaa tgcgttatca atgaccgcat cggtgaagtc
    16441 agcttttgct tcaccccagt ccatcaccac cattgggata tggcgatact cttccagcat
    16501 cgccagcaac ggctctgggt actcagaaca catcaccagc agaccatcga cgcgtttttg
    16561 cgccatcatc gacagataag cccgctgttt ctcaagattg ttccacgcat tgcccagaat
    16621 cagggtgtaa cctttctgga agcaattttt ttcaaccgct tcaatgatct cggcaaaata
    16681 ggccgcttcg ctgctggtcg ccagcaaacc gatagacttg gtgtggttaa ccttcaggct
    16741 acgcgccacc gcgctagggg agtagtgtaa ttctttaatc gctgcccaca cggcgttgcg
    16801 cgtttcttca gcgacgaaac gtgttttgtt gatcacgtgt gacacagttg tagtggaaac
    16861 gtttgctcgt ttcgctacat cttttattgt tgccattcca tttcactcca gaccctaact
    16921 tcagctcctg caaaatcgca aggtaaacgt ttgcctttac acaccttacg cacaacaaaa
    16981 gtgttgcggt acgccggaaa aacgacacgg agcgtcagga aggggtcaat ggccagtacg
    17041 caaatagtgt aagcgtggaa ttttggccga tcttgacgaa aaggggaagt ggtaaaaaca
    17101 cttatcatta taaatgacgg gagatttttt catcacagtg tgtaaaaatg cgtaatatca
    17161 ctttttgtgt ggtaataaaa aaggagaaaa cttgatgagc accgacctta aattttcact
    17221 ggtaacaacg attatcgtcc tcggtttgat cgtggtcgtg ggtttgactg ccgcgctgca
    17281 ctgatttcct ctcgagggag cgcgttctcc ctcgcatctc cttcctgttt gttagcaaaa
    17341 tggcaaaagt gttttctctt tttgttattt tcattttttt gtgattgctg tcatgctttc
    17401 gccactttaa tgaatatcgc gtaatgacgc gaagaggtgg agataaggca tgaaaattaa
    17461 ctatccgttg ctggcgctgg cgattggcgc gtttggtatc gggacaacgg agttctcgcc
    17521 aatgggcttg ttgcccgtca ttgcgcgcgg tgtggatgtc tcgattcccg ctgccggaat
    17581 gttaatcagt gcttatgcag ttggcgtaat ggttggcgcg ccgctgatga cgcttctact
    17641 ttctcatcgt gcccgccgca gtgcgttgat tttcctgatg gcgattttca cgcttggcaa
    17701 cgtactttcc gccatcgcgc cggattatat gaccctgatg ctttcacgca ttttgaccag
    17761 cctgaatcac ggggcatttt ttggtttggg ttcagtcgtg gccgcaagcg tggtgccaaa
    17821 acataaacag gccagcgcag ttgccactat gtttatgggg ttaaccctgg caaatatcgg
    17881 tggcgtgccg gcggcgacct ggttgggtga aaccatcggc tggcggatgt catttctggc
    17941 aacggcgggg ctgggagtga tttcaatggt aagtctgttc ttctcattac ctaaaggtgg
    18001 tgcaggggca cgacctgaag tgaaaaaaga gctggcggta ttaatgcgtc cgcaggtgct
    18061 gtctgcattg ctgacgacgg tactgggagc tggtgcaatg tttaccctct acacctatat
    18121 ctctccggta ctgcaaagta ttacccacgc aacaccggtg ttcgtcacag caatgctggt
    18181 gctgattggt gtcggattct ctatcggtaa ctatctcggc ggcaaactgg cagatcgttc
    18241 agttaacggc acgttgaaag gctttttgtt gctgctgatg gtgattatgc tggcaatccc
    18301 gttcctggcc cgcaatgagt tcggcgcagc tattagcatg gtggtgtggg gcgctgcaac
    18361 ctttgcggtc gtaccgccgt tacagatgcg cgtgatgcgt gtcgccagtg aagcgccagg
    18421 tctgtcttca tcagtcaata ttggtgcctt taatcttgga aatgcgctgg gagcagctgc
    18481 tggtggtgcg gtaatttccg ctgggctggg atacagcttt gtgccggtga tgggggcgat
    18541 tgtcgcggga ctggcattat tgctggtgtt tatgtcagcc agaaaacaac ctgaaacagt
    18601 ttgcgttgcc aacagctaat taaacaaaaa cgcagaagga taaccttctg cgttacagtt
    18661 gctggcaacg tgcgatttat cttgccgaat gcgctgttaa cgcctcatcc gtgttggcat
    18721 ggcgcgcgaa gcgcatcagg taatgttgca tttgccatca gttattatgc agcgagattt
    18781 ttcgctacga attcccagtt caccagcgcc cagaagtgct ccagatagcc aggacgtgca
    18841 ttgcgatagt cgatgtaata agcgtgttcc cagacatcaa cggtcagcag cggagttgca
    18901 tcggtggtca gtggagtacc cgcgttagag gttgaaacga tagccagttt gccatcgctg
    18961 tttttcacca gccaggtcca gccagaacca aagtttttga tcgctgcatc agtaaactga
    19021 gctttgaaat cggcaaagct gccaaaagat gcggcgatag cttcagcgac ttttccagtc
    19081 ggttcgccac ccgcgttcgg tgccaggcag ttccagtaga aagtatggtt ccagacctga
    19141 gctgcgttgt tgaatacgcc accttcagag ctgcgaataa tctcttccag tgatttacct
    19201 tcaaacgcgg tacctttaat caggttgttc aggttagtga cataagtctg atgatgcttg
    19261 ccatagtgat actcgatggt ttccgcagaa atgtgcggtg ccagagcatc tttagcatat
    19321 ggtagtgcag gtaattcgaa tgacattgct actctccttt attattaatt tgcatacgta
    19381 caatagcctt attgtgcgta tggatagggt agcaaattaa aaggtgagac aaaagatgaa
    19441 cttaccctgt tgccgtaaca acagggtaaa gttttagcga agtgtttttg gggtcattac
    19501 ccgacgagcg ccaacatagt ggcgctgcca gtagtcttca ctgagagaag tgatttgaat
    19561 ttcttggccc gtgcgcggtg actgaataaa tttgccgttg ccgacataca cgccgacatg
    19621 atcggctgta ccgcgtccct gagtacggaa gaagaccagg tcgccgtttt tcagttcact
    19681 acgttcgata ggtgctgcat cacgcaggtg atacatttca ttcgccgtac gcggaatacg
    19741 aattttcacc aaatctttat aagcgtaata aaccaggccg ctgcaatcaa aaccggtacg
    19801 cggtgagctg ccgccccaac gatatggctt accaatttgt tgcatcagtt tattcattgc
    19861 cactttcgtg gctttttgca ccttcgcttt gtgcgcgtcg gcaagggttc ctgacgcggc
    19921 atttttgact ttcacacaat gcgatttata gcccttacgt ttggtacact tttcggtaaa
    19981 gttggctgag gccgttttat tgacggcatg tttgctgcga gttttggcgt ttttagaagc
    20041 ggtaatggaa gattttttaa cggaagaagc ggttttgctt ttggtcgtac ttttactggc
    20101 ggcttttttc gctgttttct ggctggcttt ctgggtcttt ttggtggttg ccgtgctttt
    20161 ctttttatct gcttttttgg tgatatgagt agtagcagaa ctctccctgg cttgctttga
    20221 agcatgggcc ataggcgtta aaggcagggt ggtaaaaagt aaagcacaga gcgtgatcga
    20281 aatacggttt atccgcgcca ctgagcgtcc ctctgttaag gcaggtctat catgcctgcg
    20341 tctgatttac aaaacttgat gattctaatg catagaagaa gagaaagtta atagctttta
    20401 ttgttaaaaa atacgtttcg ttggcgagcg caaaaaaatg cagcagtgaa agctaaaaat
    20461 ggttataaat acgccagttt gccgtgcgtc agggaagaaa cgaatatttg aaatgcaaca
    20521 aatagagcaa agggataaaa tagcaaaagc gtgatgaaaa tgttattttc cgatgccgca
    20581 ctgacccgct acaatgtcag attactgccg taaagaagtt tgaggaagca agacaatgag
    20641 caccactatc gaaaaaatcc aacgccagat tgctgaaaac ccgatcctgc tgtacatgaa
    20701 aggttcaccg aaactgccga gctgcggttt ctctgcccag gcagtccagg cgcttgccgc
    20761 atgtggcgaa cgttttgcct atgttgatat cctgcagaat ccggacattc gtgcggaact
    20821 gccgaaatat gctaactggc cgaccttccc gcaactgtgg gttgacggcg agctggtcgg
    20881 cggttgtgat atcgtgatcg aaatgtatca gcgtggcgaa ctgcagcagc tgatcaaaga
    20941 aactgccgct aaatacaagt ctgaagagcc ggacgctgaa taatccgtcc tgcaatactg
    21001 acggatgcga acgggcattc gtcagtcctt tctctatccc caatccagcc cttgtggcga
    21061 acttgaaaat cccgcctcac gcagcagcgt aaacatcggc gtttgccgga ccggtaggtc
    21121 attcacttct gttagcgtaa agcgcagccg tggttcgcga cgcagtgcgg tagtcagcgc
    21181 gtggaaaatc tctggggcga gtaattcctc tttttcctgc cagaccagca tttttttgcc
    21241 accttgcgcc agatagagta acaatttgcc gccagaaacg accaccagcg cacccgcccg
    21301 acgcgttggt atcagcgttg ccggatgtgc aggccagggc agaagatttc cccatgcatt
    21361 agccggatcg ttggcagaga gcgccactgg tgtatagtgg cgcgtttgcg tggcttgtgt
    21421 cgccagatcg cgcaatcggt caatagtcag acgttcagcg aattgcgcgc cacccagacc
    21481 ttctacaaaa cgacctcgca taattcgccc ggagtcttcc atacttcgac aaagcgtttg
    21541 catcgatgga aacccgccag ggatattttc ggctatcacc gcctgacgac tgatgatgcc
    21601 gtagcggtcg agcatatttt ccgccagcgc cagcatcctt tcggtatcgt ttagtggctc
    21661 cacctgcaat aacgaccagc gtccagcaag ctttggtgtg ttgtaagata ccagcggcga
    21721 gacgggttgc gcatagacag gacgtccacg acgagcccgg tgactgcggc gagttgaggt
    21781 gcgtgtgttg gtactgctgc gggtgagggc gcgtaacggt gcccaaatgt cgctggtgat
    21841 gacgccttgc cagaccagcg cccataacgc ctcttgcatg gctgagggat caaccgattc
    21901 gccgatttta tcgcgtatcc gttggctgat ttgttgtgca aaccaggctc ctccgtcagc
    21961 cagaaccgtg actatcgctt gctgcagcgc cgaactattc gcctgattcg cttcggcggg
    22021 agtgaacgat tctgcggcat attcctgtag atgcagtgcc accagaccgt catcttcacc
    22081 cagctttttt tgccccgacc agataaccgc accggttgcc agtaattcat cgagcatttc
    22141 tggcgaatag tcgcgtacgc gggcaggcag gatctggctt tcccagagtg aggcgggtaa
    22201 accgactccg gcaagctgtt cgatcacccg catgacgcca tccacgccct cataaacgcc
    22261 tggcgatgtt gaggcaaaga gtgccgggct accatcggtg gcgggtaata cgccctgacg
    22321 ttccagcagc aatcgcgcat aggtcgtggc cgcaacggga cgcgtcgctt ctctggcggc
    22381 ttgcagcgag cgcaaacgca gacgacgaaa tacttcatcg ctcacccaga tgtcttgttg
    22441 cagattcatc accagcccct gttcgcgcag ttgctgaagt tgctcttcga caatggcaat
    22501 accgagacta aactcatgag ccagttgttc agacgtgacc agagcatgag cccggagata
    22561 gcgcagaaag aggtcgcgaa gcgggtaact gactcggtgc aaataaatct ctggcagtga
    22621 ctctggtagt cgtacgccga gagcatcgcg cagcctggcg gcatcatcca tacaggcaag
    22681 acgctcctgc ccgctaatca tggtcgggaa gattcgtttt actgcaagaa gattttccag
    22741 atacgacgca atctcttcac tgctgcctgt atgtcgttgc gccaggtctt caacggtcat
    22801 tggccccagt tcgcgcagca ggtcgaacaa tccttcttca cctttcgctc ttctgccagg
    22861 agccagtcgt tgcaactctt cttccacctg gcgaatgacc tgtgggtcga gtaattcccc
    22921 cggatcgacc tgtccgagta gattgcgcag taactcgctg tccagcgaca gtacggatgc
    22981 ccggcgctct gccagcgggg cgtcgctctg gtacataaat tccgcgacat agccgaacaa
    23041 taaactggcg gcgaaaggcg aaggcgtagt ggtcgttaca tcggatattt gaatttcgcc
    23101 accgttcagg cgacgcatca aacgttccag cgcgggaaga tcataaacat cttgcagaca
    23161 ttcgcgtagg gtttcgagaa tgaccggaaa atctggatat ccctgagcga tttccagcaa
    23221 ctgactggcg cgcagccgtt gttgccagag cggggtgcga tggcccggcg tgcggcccgg
    23281 cattaataat gtccgcgcgg cgcattcgcg aaaacgggcg gcgaaaagtg ccgagctgcc
    23341 taccgcctcg cggacaattt gcagcaactt ttctggttca aacaaaaaaa tcgcggcatc
    23401 aggcaattta ccatcggtgt caggaatgcg cgcaacaatg ccgtcatcac tggcaaccac
    23461 agacgcgtca gcgccccata gtgcatgtat tcgcccggca atcgccagcg cccagggttc
    23521 atgcacccgt cttccatagg gagagtgcaa aataatacgc cagtcaccaa tttcatcacg
    23581 gcaccgttcg aggaccagat gacgactccc cggaacgatg cccgtcgcgt tgcgctgctc
    23641 gtcaatcaac ccctgaatat tggcgatcgt attttcttct gccaaccacg ggggaatagt
    23701 cccggaaaag aacgcgccat ccgccagtaa atggagaaaa tcgccgatca tctcgcctaa
    23761 ttttgccgga cgtccgttac cttcaccacg ccagaaggga aggcgggcag aacgacctgg
    23821 agcaggagtc acaatcacct gatcgcgggt gatttgctgg atccgccatg aggtagcgcc
    23881 gagagtgata atgtcgttca cccgcgactc atataccatc tcctcatcca gttcacccac
    23941 ccgccgcgaa ccggcctgtt cttcaccttc gggtaataac acgctataca tgccacgatc
    24001 cggaatggtg ccaccgctgg taaccgccaa cagctgagcg ccaggtcggg cggtcaatat
    24061 cccggtctcc ctgttccaga ccagttttgg tcgaaaagca gaaaaatcgc cagagggata
    24121 gcgcccggaa agcatatcca gcgtgacgtc aaaaacacgt cttggcagat ctttccacgg
    24181 tgcggcacgg cgtacgcggg agtaccattc gtctgcctgt aatgcatcca tcgccgcggc
    24241 ggcaacggtt tgctgcgcaa ggacgtcgag tggattatgc ggcggtgtca ggttttccag
    24301 cctgcctgcg aacatacact ctacaatgac tgcggaatcg actaagtcac gcctggttcg
    24361 ggggaaaaaa agccctttag atacaccgcc aacctgatgt ccggcgcgac caatgcgttg
    24421 taacccactg gcaacagaaa gcggcgttgc cacctgaatc accagatcca ccgcgcccat
    24481 atcaatcccc agttcgagac tggaggttgc gaccacacag cgtaattcac ccgatttcaa
    24541 cgcctgttcg gtgattgctc gttgttcttt agagacggag ccgtggtgtg agcgggcaat
    24601 aaaaatgtcg ctactttgta cacggttaga ggttgcgccg gaggtcgact cgaaatgggc
    24661 cgcatcaacg gcgatagatg gggaacgctg taagcgtgcg gcgtaaagct catttaatcg
    24721 tgccgtcagt ttttccgcca gcccgcgtga attagtaaag acaatggtcg agcgatggcg
    24781 caaaacttca tcaaggatac ccgtttcaat atatggccag atggagcctt cccggccggc
    24841 atggctgtct tcgccggtgc cgctggcgac cgatgagaca tcatccatat tggcgaccgg
    24901 tacgacaatc cgtatctgcg gatggcgcat tgcgggcggg ttgactaccg taaccgggcg
    24961 atcgccacca agaaatgctg ccacatcgct ggctgaacgc acagtggcag aaaggccaat
    25021 tcgctgtgct gaggtgtgga gcagcgtatc gagccgctcc atacttaacg ccagatgcgc
    25081 accacgttta ctgcccgcta ccgcgtggac ttcatcaata attaccgttt cgacgccgcg
    25141 tagcgtttcg cgcgcgcggg aggtcagcat cagatagagt gattcgggtg tggtgatcag
    25201 aatatccggc ggattacggg tgagtttgct gcgttcctgt gcaggcgtat cgccagtacg
    25261 gatccccacg cgaagattga cttccgtttc gccgcgccgc cgccgttcat cggcaatacc
    25321 ctgtaacggg atctgcaagt tgcgctgaac gtcggtgccc agagctttga tcggtgaaat
    25381 atagaggatg cgtgaggttt tacgcttatg cgcctcgcgg gtatcttcgc cgccttcgcg
    25441 gaagagctga tcgagggcgt agagaaatgc tgccagcgtt ttcccggagc cagtcggtgc
    25501 aatcaccagc gcatgttcgc ctcgcgccgc cacatgccag gtttgcgact ggacagcggt
    25561 cggctgttta aaggcgcgaa gaaaccagtc gcgggtcgcc ggtgaaaaca cgtccggcag
    25621 gggcgatgaa gggtttagat tatctgccat taacgctatt ccccgtgaat atcatgcatg
    25681 atgcgtggaa tagcctcact atagatgcct gaatcattgc atgtcatcag gcatcgactc
    25741 gattacacct cttcggcggc aggtagcggc cagcctccca gacgtttcca gcggttgacg
    25801 atttcacaaa acagcacagc agtgcgttcg gtgtcgtaca gcgcggagtg cgcctgggtg
    25861 ctgtcgaagt ccatgccagc ggtctggcaa gcctttgaca atacggtttg tccgagtgcc
    25921 agcccggcca gtgcagcagt gtcaaaagtg gcgaaagggt ggaacgggtt acgtttcagt
    25981 gaggcgcgtt ctgcggcggc catcataaag ctgtgatcga aattggcgtt gtgcgccacc
    26041 ataatggcgc ggttacaacc gctcgcttta atacctttac gcacaacttt aaaaatttcg
    26101 tgcagcgcct cgtattcgct gaccgcgccg cgatcgggat cgttcgggtc aatgccgttg
    26161 aaggcgaggg cctctggctg caaatttgcg ccgacaaatg gttcgacgtg gaaatgtaat
    26221 gtggtgtccg gcatcagcca gccttgttca tccattttca gggtgatggc ggcaatctca
    26281 agcagcgcat cggttttggc gttaaatcca gctgtttcaa catcgatcac aacaggataa
    26341 aaaccacgaa aacggtcgca cagaccggta agttgagcgt tatcggacat ctgattctct
    26401 taatacggat aaattgcagc gcgcattatg acaaatatta cttgtcgatg cagtaaagat
    26461 gcgggcgcga tgagttcacg cccggcagga gattagttgc ccagaccgcg accggcgtct
    26521 ttctcttcga ttaactcaat tttgtaaccg tccggatctt ccacaaacgc gataaccgta
    26581 gtgccgcctt ttaccggacc cgcttcacgg gtgacgttac cgccgttctg acggattttt
    26641 tcgcacgctt cagcggcgtt atctacgcta agcgcgatgt gaccataagc agtgccgagt
    26701 tcgtatttat ccacgcccca gttgtaggtc agttcaatca ccgcttcttc ggtttccggg
    26761 ccgtagccaa caaacgccag tgagtatttg tattccgggt tttcgctggt acgcagcagt
    26821 ttcatgccca gtactttggt ataaaaatcg atggagcgtt gcagatcgcc aacgcgcagc
    26881 atggtatgaa gaagacgcat ctttatatcc tcaaaatggc taacagattc aaaatgttgt
    26941 tttagtatag cggcggcttt gcgccgctcg caatgttgga ttacagcgtc gggtaatcag
    27001 tgtagccttc cgcgccgcca ccgtaaaaac tttcggcacg ctgtgggtta agctcagctt
    27061 tgcgttgcaa gcgggcgacc agatccgggt tcgcaatcca gtcacgacca aatgccaccg
    27121 catcaattaa ccctttgccg atcagcgttt cggctttttc taccgtgtat gcacctgcgc
    27181 cgataatcgg accgtggaaa cgggcgcgta ctttttcgcg gaacgcatca gtatacggtt
    27241 taccccccgc ccaatctggt tctgacatat gcagataagc aatgccgcgt ttacccagtt
    27301 gttctatcag atacagtgca tcggcttctt cattcgggcc gttatctgtg ttctggaaag
    27361 taccgattgg cgaaacgcga atgccaatgc gatcggcacc ccattcttca atcccggcat
    27421 cgaccacttc cagcaccaga cgtgcgcgat tttccacgct gccgccgtac tgatcggtac
    27481 gatggtttga agaaggagaa aggaactgat gcagcaaata accatgagca gagtggagct
    27541 ctaccagatc aaaaccggct tcacgcgcgt tagcaatggc ctgacggaaa tcattgacga
    27601 tacccggaat ctcttccagt tcaagcgcac gcggcatgga tgtttcaaca cggatcgcct
    27661 gaccattttc atcgcgcaga gaagtacgtg ttcccgcgct aagtgcggaa ggcgctaccg
    27721 gtgcctgacc gccaggttgc aggctggcgt gagaaatgcg tccggtgtgc cacagctgta
    27781 cggccatatg accattttca gcatgaacgc cagcggtaat ttttttccat gcggcaattt
    27841 gttccggact atggatgcca ggtgcaccag catacccttt tgcctgggca gaaatttgcg
    27901 tggcttcgct aataatcaaa ccggcactgg cacgttggcg atagtattcc gccatcaacg
    27961 gggtagggat gtcacccggt tcaatactgc gcagacgcgt cagcggtgcc ataaaaatac
    28021 ggtttgccgc cgtgatcgcg cccactttca gtggggaata cagtttttca gatgacataa
    28081 aggctcccgg tcgtctggtg ataaaaaatg cctgctaaac ggcaggcgtc gcaataatgg
    28141 tttttacatg tgccagcgcg ttttccagtg gctcgaaact gcgcgaaatt ttggcctgca
    28201 gattcgcacc cagccatagt gcgtaaagca cttgtgcctg ttgcagcggt tcgccacaaa
    28261 aggttaaaca atggctatca cggccatttt ccagcgcctg cgagagcagg gcgatcacgc
    28321 cgcgagcgcc tttatccatc gcgctacgca tatcttctga cagatcgcac acttcggcag
    28381 agagttttac tgtcaggcaa ccactgatgg ttccatgttg gcaaaactgg ttcagtgttt
    28441 gctggtaata agccagtatg cggtcgcggt agttaccttc gccggattgc agcaactcag
    28501 tcagtcgttg gtgatatgtg gcgtaatggc gctcaagcat ggcaacgcca aacgcttctt
    28561 tagagcgaaa gtagtgatag aaggaccctt tcggcacttc agcggttttt agtaattcgc
    28621 ttagccccat cccggtgaat ccacgttgca ggcaaagttg ctcgcccgtc gccaggagat
    28681 gttcgcgagt atcatgttcg gtgtgtttgt tcatgcgttg gagtgtagta gaccagtcgg
    28741 tctagtgcaa gcaaagttgc tactttgggc attaacagct tcaataatgg taataatctt
    28801 atttgaggag ggcgaagtgg cggagcaatt agagttcttt cccgttcaga gcccgtgccg
    28861 gggaatttgc cagtctgacg aacgcgggtt ttgtcgcggc tgttttcgta gtcgggatga
    28921 gcgttttaac tggaataaaa tgagcgatgg cgaaaaacaa gaggtgctgc gcctttgtcg
    28981 gcagcgatta atgcgcaaat tacgcgcaaa taagccagcg tcatcagacg aaccggagca
    29041 accatcactc ttttgagcct aatcttgcgt atactcctct catatctgat aagaggaagc
    29101 gattatggtt cagcgtatta ctattgcgcc gcaaggcccg gagttttccc gttttgtgat
    29161 gggctactgg cgattgatgg actggaatat gtccgcccgc cagctggtca gttttattga
    29221 agagcatctg gatctcggcg tgaccaccgt ggaccatgct gatatttatg gtggctatca
    29281 gtgcgaagcg gcgtttggcg aggcgctgaa actggctcct caccttcgtg aacggatgga
    29341 aatcgtcagt aaatgcggta tcgcgacgac cgcgcgtgaa gaaaacgtca ttggtcatta
    29401 cattactgac cgcgatcaca tcattaagag cgccgaacag tcgctaatta atcttgcgac
    29461 cgattatctg gatctgctgt taatccaccg accagacccg ttaatggatg ccgatgaagt
    29521 ggcggacgcg tttaaacatc tgcatcagag cggcaaagtg cgccattttg gcgtatcgaa
    29581 ctttacgcct gcgcaatttg ccctgttgca atcacgtctg ccgtttaccc ttgccactaa
    29641 tcaggtggaa atatccccgg tgcatcagcc gttactgctg gatggcacgc tcgaccaact
    29701 gcaacaactg cgtgttcgtc cgatggcgtg gtcctgcctt ggtggtggtc gtctgtttaa
    29761 tgatgattat ttccagccgc tgcgtgatga actggctgtg gtggcggagg agttaaacgc
    29821 gggctcgatt gaacaggtgg tttacgcctg ggtattacgt ttaccgtcgc agccgctgcc
    29881 aattatcggc tcaggtaaaa ttgagcgtgt acgggcagct gtcgaagctg aaacactgaa
    29941 aatgacccgt caacaatggt tccgtatccg taaagcggca ttgggttacg acgtaccgta
    30001 agcactttta ggaatagccg ccgttcaaaa atgtgtcact ggtttacact tattcaggaa
    30061 tgcacaatga acggaggtcc tatgaaacgt tttagtctgg ccattctggc gctggttgtt
    30121 gcgaccggcg cacaagctgc cagtgaaaaa gtcgagatga acctcgtcac gtcgcaaggg
    30181 gttgggcagt caattggtag cgtcaccatt actgaaaccg ataaaggtct ggagttttcg
    30241 cccgatctga aagcattacc ccccggtgaa catggcttcc atattcatgc caaaggaagc
    30301 tgccagccag ccaccaaaga tggcaaagcc agcgccgcgg aatccgcagg cgggcatctt
    30361 gatccacaaa ataccggtaa acatgaaggg ccagaaggtg ccgggcattt aggcgatctg
    30421 cctgcactgg tcgtcaataa tgacggcaaa gctaccgatg ccgtcatcgc acctcgtctg
    30481 aaatcactgg atgaagtcaa agacaaagca ctgatggtcc acgttggcgg cgataatatg
    30541 tccgatcaac ctaaaccgct gggcggtggc ggtgaacgct atgcctgtgg tgtaattaag
    30601 taattaagag gccagcgtac cttgtggcgg tgcttgctca agttgcgaaa gcgagcagta
    30661 cagccgccag acaattgccg ccagctcgcg ggcggcaggt tgatgatggc gggcaaggct
    30721 gtcgcaaatc cgctgtaatt cttcaagtgt ggccgccagt gatttttgct gaacgccacg
    30781 ctcactcatc actccccgca acagtgaaat acagttatcc cgtactcgcg ataacggatc
    30841 ggaacgcgat tcccagtcgc gcaattgcca gacaacatga gaacagttca gcagcactac
    30901 accccagcgt aataaccaac ggcgagccag cgcatcctgg ctgttactca actgactgac
    30961 gtgatgataa gtgagcgatt caaattcgct ttcactcagt gttggatggc ggcttagttg
    31021 atcgacaaaa tcccggcgca gcgcgcgtat atggcggcgg cttttgcgag catccgatcc
    31081 tggacgcaga atggcgaacg ctaaccacgc caacgcgacg ccaacgattt ttgccagatt
    31141 atcgttaaga aaatcagcaa aatcatacac cgggggatta gtgacagcga taaacgaacc
    31201 cataaaaaca atcagttgcc cccacagtgc ggcaaatttg ggcatctgca atttaagaag
    31261 ctgcattgtc gccagcagtg gaaagagaaa cagtaaaaat tgccacagat cgctaatctg
    31321 caccatcagg ccaaatttga ccacaaagct gaatagcgaa agtaacacca gcgtgcgcat
    31381 cagcagtgac aacgacttaa acggtgctgc gacggcggag tagagtacgc agctaattgc
    31441 tgccagcgtt aatgcattgg aaccggcatc ccattgcgaa gcaatactcc atgcgccaat
    31501 catcatcaac gtacaaaatg tacgcagtcc gctccacata gcttcggcgt tatcggtatg
    31561 acgagccagg ccggatgtcc gtgggagtct ggtatgatca tccacaccgc tttgcagatg
    31621 ttgtaattcc tgactacttt gcagataaag gcggcaaaaa taacgtagtc gctgccagaa
    31681 ggcgacgtgc cgatagtcgg cgacgttggt cgggcgtagc ggggcgataa tacgtgcgac
    31741 ggtgtaaaca tctgtttgcg aactggcgag cgccgtcagc agctgttcga gaatttctcg
    31801 tgtggcacat ggcggtgagg gccagttgag caacatacgt cgcaggctgg agatgacact
    31861 ggtcatacgt aattgctggt ggagcagcgc attaaggcgt gcgttttgct ggcgaaaacg
    31921 atagtggctc cagaaagcct ggatacggag caaattcatg gtcagtatct gtccaatcac
    31981 cccctcatgt gctgcacgaa tggcatcggt tgtttcaggc tgccagagta aactggcatg
    32041 ttccagtaat cgggcgtgca tgtttttcaa tgcggttaaa agggctgtag catcggaact
    32101 gctcggcagg atcatcatca tcatgccgcc gcacaaaata ccgacaatca cctcgcaaac
    32161 gcgcgcctga gcgatatccc acagctggct ggcctcagta atattaacca tcggaaaggc
    32221 gatgatggca gctgtgtagc ccgccagttg aaatgcatac gcgacattat tcgtgaagtg
    32281 cgcacaggcc caggtacaaa agccaagcca cgccgccatg ctcaaaagaa aaaaccacgg
    32341 ctcattgagc gtatgtccgg caagaagcag tgccgcaatg gctccgagca aactgccagc
    32401 gatgcgtccg aggcttttgc tgatgacacc gccaacggtg ggaaagctaa ccacggcagc
    32461 cgaggtcatc gcccaatagg gttcatccag atttaaataa taggcaaccg tcagcgccag
    32521 acacatggca atggtattgc gtaacgcata acgccattgc gccagcgtgg ccctgaacca
    32581 gggcaaattg cgcaaggacc atgacgatgc gttcattatt gacctacggc aatactacag
    32641 gtggtgcccg acaccagcgt gacatcgcct gggactttat ccagcgcaaa gcggacggga
    32701 actcgttggg cgaggcgaac ccaggggacg ttaggtttca catccggaat aaggtttgag
    32761 tcactttcaa cgctctgatc ataaatcgcc cgaccgatac tggaaacgtg accctgtaac
    32821 gttttgttat cactatagag ggtaatttgt gctggtgcac cttcgcggat atggcgcaac
    32881 ttggtttctt caaaataacc aatgacgtaa aacgagtggc tgtcgacaag ggcaaacagc
    32941 ggtttcccgg tgtcagcgta atcgccgatg cgggtagtga ggttagtcac ccatccactc
    33001 accggggcgc gaatttccgt ttgcgccagt tgccattgtg cctgtttcag cgtcgcctgt
    33061 gcggcatcaa cactggcctg catcgcttta acattgaggt tagcggtatc gagttcttcg
    33121 gcagaaataa aattttgtga gagatgacga cgacgattcg cttcgttatt ggcttttgcg
    33181 aggtcagatt gcgctttcgc caactgagcc tgtgcgttca gctcggcgat ctgaaagggc
    33241 gttttatcga tcgtgagcag aagatccccc gcgttaacca gttggttgtc ttttatattc
    33301 agctcaacaa tacgtcccga cacctgaggg gtgatagaaa cctgttctgc gcgtattttc
    33361 ccgtcgcgtg tccacggaga ttgcatgtaa taattccaca gccaccatcc ggcaagaacc
    33421 gcaactaccg ctacaatgat tgttgagaaa tacttaattg ttttaattga catagttacc
    33481 acgcaatcag tattgccagg gaaaggcaaa cgcaaatcgc aaacagcgat aaatccatta
    33541 acaagggatg ccagatgtca ccggcgtaga tccagccacg aagcaggcgg tgtacgacaa
    33601 gccagatgac gaatccaaac gcgaaggctt tgaaaaacgg aggaaagtag acggacgcac
    33661 cgaacaccag atcttgtaag ggcaatcctg ttgcattgag cataaacttc acaaagtaga
    33721 ttcctttacg accgaatata ttgcgttaga ttaataaata ttctttaagt gcgaaaaatt
    33781 tacgcgcaat ttctgaaaaa tatgtctgac ggtaaccaaa tgcagcaata catttgtttt
    33841 agcaatacaa ttgctgcaca ctattctaaa agccgcataa tatcttagca agctaattat
    33901 aaggagatga aattggaatc gccactaggt tctgatctgg cacggttggt gcgcatatgg
    33961 cgtgctctga tagaccatcg cctgaaaccg ctggagttaa cacaaaccca ttgggttacg
    34021 ttacacaata tccatcagtt acctccagac cagtcgcaaa ttcaactggc aaaagcgatt
    34081 ggcatcgagc agccatcact ggtccgtact ctggaccaac tggaagaaaa agggttaatt
    34141 tcgcgtcaaa cttgtgccag cgatcgtcgg gctaaacgta ttaaactgac ggaaaaggca
    34201 gagccgctga tcagcgaaat ggaagctgtt attaacaaaa cccgcgcgga aattttacat
    34261 ggcatctccg cagaggaact ggaacaactg attaagctca tcgcaaaact tgagcataat
    34321 atcattgagt tacaggccaa agggtgaaat gaagggggcg tgtggtcagt aacctgacca
    34381 cacgcaaact tatttagcgc ggggaaacgg tcacctgact gccattgctg gccagtacga
    34441 cacgttggcc cggagagaaa cgagtgttgc cttgtttctg taccaccatg atggtattac
    34501 catcgtcttt acgaatttcc agctcgacac cctgcgtttt gttcattgca ctctgtacgc
    34561 cctgaccggc tacgccacct gcaacagcgc ctgcagcagt agccagagaa cgcccggttc
    34621 cgccaccaac agtattcccc aggaaaccac caagaacagc accgccaatt gcgccgataa
    34681 cgttggaatc atcaccgccc tgaatctgta ccggacgtac gttaacgatg gtgccatagc
    34741 tgacattctg tacttgtttc gcttcagaag cggtataaac atcccctgac agggtgtcgt
    34801 tattaacaca accgacaaga gacagaccta ccattgaaac aaccaatacg cgtttaatca
    34861 ttgaaaaatc tcctgttcac catgaaacgc cacgcaagca tccctcatgg tcaaagtata
    34921 tggcatatgt gaatccatgg ttacatgttc taccaaatat gagcaaatca taatgaaagt
    34981 aggatcaacc aattataaac aaaacattca tgatgattat tgatttgaag ataatgtttt
    35041 actaccaacg gtaaaaatcg gtcattccgc atggtattca cgaaaagttt atgtttgagt
    35101 ggtgcgcaaa cgtcagattg aaaaaggatg aactatgaaa tcgggccgct ttattggcgt
    35161 tatgtcaggc accagccttg atggtgttga tgttgtgttg gcgacaattg atgaacaccg
    35221 ggtcgcacag ctggcaagtt tgagctggcc gatcccggtg tctctgaaac aggctgtact
    35281 ggatatttgc cagggccagc agcttatact ttctcagttt ggacagcttg atactcaact
    35341 cgggcgactt tttgctgatg cggtcaatgc cttgcttaag gaacaaaacc tgcaggcgag
    35401 agatatcgtt gcgatcggct gtcacggtca aaccgtctgg catgaaccga cgggcgtggc
    35461 accacacact ttacagattg gcgataacaa tcaaattgtg gcacgcaccg gaattacggt
    35521 tgtcggtgat tttcgccgtc gcgatattgc cttgggagga caaggcgcac cgctggtacc
    35581 tgcgttccat catgcgctgc tggctcaccc aaccgagcga cgaatggtgc tcaatattgg
    35641 cggcatcgcc aatctgtcac tgctcattcc tgggcagccg gttgggggct acgataccgg
    35701 tcctggtaac atgctgatgg atgcctggat ctggcgtcag gccggtaaac cttacgataa
    35761 agatgccgag tgggcacggg cgggtaaagt tattctccca ctgctgcaaa atatgctcag
    35821 cgacccgtat ttctcgcaac ccgcaccgaa aagcaccgga cgcgaatact ttaactacgg
    35881 ctggctggag cgccatttgc gccattttcc gggtgttgat ccccgtgatg tgcaggccac
    35941 actggcagaa ctcaccgccg tgaccatttc tgaacaagtt ttgttgagcg gtggctgtga
    36001 acgattgatg gtatgtggtg gaggaagtcg taatccgcta ctcatggcgc gtctggcggc
    36061 attactgcca ggcacagaag tcaccaccac tgatgccgtt ggcattagtg gcgatgacat
    36121 ggaagcattg gctttcgcct ggcttgcctg gcggacgctt gcgggattac caggaaatct
    36181 gccttccgtc actggcgcaa gccaggagac ggtactcggg gctattttcc ccgctaaccc
    36241 gtgatatcag agttaactga atttttctta ccgcgtctgc cgttacactt ggaacgttag
    36301 ggagggcgta ttgccctcca gaccaggaaa gtcttcggga tatgtctatg aaaaaactgt
    36361 tactgatctg tttgccggtc cttctcaccg gctgtagcgc gttcaatcaa ctgtttgagc
    36421 gcatgcaaac cgatacgctg gaataccagt gtgatgaaaa accgttgacg gtcaaactga
    36481 ataatccgcg ccaggaagtc agttttgttt acgataatca actactgcat ctcaaacagg
    36541 gcatttctgc gtctggtgca cgttacaccg acgggatcta tgttttctgg tcgaaaggcg
    36601 aggaagcgac tgtctataaa cgcgaccgca tcgtcttgaa taactgtcag ttacaaaatc
    36661 cacagcgttg agatttttcc aggggcggcg cacaatagcg tcacccactg acaatccgta
    36721 aagaaaacca tgtctgataa cgacgaattg cagcaaatcg cgcatctgcg ccgtgaatac
    36781 accaaaggcg gattacgccg ccgcgatctt cccgccgatc cattaaccct ttttgaacgc
    36841 tggctctctc aggcgtgtga agccaaactg gcggacccta ccgcgatggt ggtcgctacc
    36901 gtggatgaac atggtcagcc ttatcagcgc atcgttttac tcaaacatta cgacgaaaaa
    36961 ggcatggtgt tttacaccaa cctcggcagc cgtaaagcac atcaaatcga aaataatccg
    37021 cgcgttagcc tgctgttccc gtggcatacc cttgagcgcc aggtgatggt gatcggtaaa
    37081 gcggaacgac tttcgactct cgaagtgatg aaatattttc atagtcgccc acgtgatagc
    37141 cagattggtg catgggtttc gaagcagtcc agtcgcattt ctgcccgcgg tatccttgaa
    37201 agtaaattcc tggagctgaa gcagaagttt caacagggcg aagtgccatt gccgagtttt
    37261 tggggcggtt ttcgcgtcag ccttgaacag attgagttct ggcagggcgg tgagcatcgc
    37321 ctgcatgacc gctttttgta ccagcgtgaa aatgatgcgt ggaagattga tcgtcttgca
    37381 ccctgaaaag atgcaaaaat cttgctttaa tcgctggtac tcctgattct ggcactttat
    37441 tctatgtcta tttcgcatct ggcgaaaagt cgtgtaccgg caaaggtgca gtcgttatat
    37501 acatggagat tttgatggca agcagtaact tgattaaaca attgcaagag cgggggctgg
    37561 tagcccaggt gacggacgag gaagcgttag cagagcgact ggcgcaaggc ccgatcgcgc
    37621 tctattgcgg cttcgatcct accgctgaca gcttgcattt ggggcatctt gttccattgt
    37681 tatgcctgaa acgcttccag caggcgggcc acaagccggt tgcgctggta ggcggcgcga
    37741 cgggtctgat tggcgacccg agcttcaaag ctgctgagcg taagctgaac accgaagaaa
    37801 ctgttcagga atgggtggac aaaatccgca agcaggttgc cccgttcctc gatttcgact
    37861 gtggagaaaa ctctgctatc gcggcgaaca actatgactg gttcggcaat atgaatgtgc
    37921 tgaccttcct gcgcgatatt ggcaaacact tctccgttaa ccagatgatc aacaaagaag
    37981 cggttaagca gcgtctcaac cgtgaagatc aggggatttc gttcactgag ttttcctaca
    38041 acctgttgca gggttatgac ttcgcctgtc tgaacaaaca gtacggtgtg gtgctgcaaa
    38101 ttggtggttc tgaccagtgg ggtaacatca cttctggtat cgacctgacc cgtcgtctgc
    38161 atcagaatca ggtgtttggc ctgaccgttc cgctgatcac caaagcagac ggcaccaaat
    38221 ttggtaaaac tgaaggcggc gcagtctggt tggatccgaa gaaaaccagc ccgtacaaat
    38281 tctaccagtt ctggatcaac acagcggatg ccgacgttta ccgcttcctg aagttcttca
    38341 ccttcatgag cattgaagag atcaacgccc tggaagaaga agataaaaac agcggtaaag
    38401 caccgcgcgc ccagtatgtg cttgcagaac aggtgacacg tctggtgcat ggtgaagaag
    38461 gtttgcaggc ggcaaaacgt attaccgaat gcctgttcag cggttccttg agtgcgctga
    38521 gtgaagcgga cttcgaacag ctggcgcagg acggcgtacc gatggttgag atggagaagg
    38581 gcgcagacct gatgcaggcg ctggtcgatt ctgaactgca accttcccgt ggtcaggcgc
    38641 gtaaaactat cgcctccaat gccatcacca ttaacggtga aaaacagtcc gatcctgaat
    38701 acttctttaa agaagaagat cgtctgtttg gtcgttttac cttattgcgt cgcggcaaaa
    38761 agaattactg tctgatttgc tggaaataat gcattaagtg gaaaggggga gtgagaaatc
    38821 actccccctg gtttttatac agggaacatg atgaaaaata ttctcgctat ccagtctcac
    38881 gttgtttatg gtcatgcggg taacagtgcg gcagagtttc cgatgcgccg cctgggcgcg
    38941 aacgtctggc cgctgaacac cgttcaattt tctaatcaca ctcaatacgg caaatggact
    39001 ggctgcgtga tgccgcccag ccatttaacc gaaattgtgc aaggcattgc cgccattgat
    39061 aaattacaca cctgtgatgc cgtattaagt ggctatctgg gttcggcgga gcagggtgaa
    39121 catattctcg gtatcgttcg tcaggtgaaa gcagcgaatc cgcaggcgaa atatttttgc
    39181 gatccggtaa tgggtcatcc ggaaaaaggc tgtatcgttg caccgggtgt cgcagagttt
    39241 catgtgcggc acggtttgcc tgccagcgat atcattgcgc cgaatctggt tgagctggaa
    39301 atactctgtg agcatgcggt aaataacgtc gaagaagcgg ttctggcagc gcgtgaactc
    39361 attgcgcaag ggccacaaat tgtgttggtt aaacacctgg cgcgggctgg ctacagccgt
    39421 gaccgttttg aaatgctgct ggttaccgcc gatgaagcct ggcatatcag ccgtccgctg
    39481 gtggattttg gtatgcgcca gccggtaggt gttggtgatg tgacgagcgg tttactgctg
    39541 gtgaaactgc ttcagggggc aacgctgcag gaggctctgg aagatgtgac cgctgcagta
    39601 tacgaaatca tggtgaccac caaagcaatg caggaatatg agctgcaagt ggttgctgct
    39661 caggatcgta ttgccaaacc agaacattat ttcagcgcaa caaagctctg aaatatctca
    39721 tttcggccca caacgacctg tgggccgatg gctattactt taagccttcc gctgacagcg
    39781 cgtcttgtac ttccggacgt tcagccatac gttgcataaa tgccgcaatg tgctctaacc
    39841 cttccagatt cagtttcacc gcgtatgccc agcgcagaac cgtaaacaga taggcatcag
    39901 caattgtaaa tcgttgcccg cagatccagt gctcatcctt cagtgcctcg ttcacatatt
    39961 gcagcttctt ctccagctgc gcgcgaactg tcgatttgta ctcttccggt gtatcagggc
    40021 gaaacagagg tgtgaaacct ttatgcagct cggtggcgat gtaattcagc cattcgatgg
    40081 ttttatagcg ggaaatactg tttaccggtg ccagcaactg gcggtcgggg acgctgtcgg
    40141 caagatactg cataatcgct acgccttccg tcagcaaagt accatcgtcc agcagcaatg
    40201 caggtacctg tcccttaggg ttaacggcaa agtaatcgtc accgttttcg agacgttttt
    40261 tcattaaatc cacactgacg agcgtaaaat cctttccgct ctcacgcagg gtgatatggg
    40321 aagcgagaga gcaggcaccc ggtttgtaga acaatttcat cggtaactcc ttttgtctgt
    40381 ggtttaaagt atggtagtgc gttgctgaac aaaaaaaaag ccgctaacga aaagttagcg
    40441 gcttgtaaaa gagtttccct gaattttacg ctacggctgc tttcgccgct ttgtctgcag
    40501 cgtcatcctg cgtcatgcgg tgcagtttcg gcgcggtcag cagcatcagt actgcaataa
    40561 cggcagtagc gacaccaatc tgcaagaata cgcgaccata gacttccagt gacatcagtg
    40621 gatcggtaac gttatccggc acagccatca tacccgcaac ataaccacca atcaggtttg
    40681 caccggcagt ggtcaggaac cagctaccca taatgaagcc catcagacgc tgcggaacga
    40741 gttgtgcaac cattgccaga cccagaccag agatcatcag ttctccgata ctctgcaggc
    40801 catagcttgc gaccagccag cttacagaca cgatacctgc gtcagatgcg aattttgcac
    40861 ccagcggcag aatcaggaac gcaccagaac acatcaccat accgattgca aacttagttg
    40921 gcatcggcag ggtatcgccc atcttgttat agatagcggc cagaatcggg ctaccaatga
    40981 tgatccagaa cgggttcagt gcctgatact gctcaggttc tacggccagg cccagaatgg
    41041 tgtgttcaac gttacgaatc gcaaagaagt tcagtgacgt tggcatctgg ctgtacagca
    41101 cgaagaagat aatggcttcg agcatcagga tgaaggcaac gatcatttta cgacgcgcag
    41161 cacctttcat cgcgaaggct tctttaccga agataaccac gataccaaag gcaacaacgc
    41221 ccagcgccat acgcgcaact tcctgattgt gcagcagcca ggtggcgata gcgatcagtg
    41281 ccacaacacc aataatggtc agcagcaggt tacggtagtt gataggctcg aagtctggtt
    41341 ttgaaccgta ctgtttaacc cagcgttggc agaaggcgaa gttaacgata gtgatcagca
    41401 ggcctacaac gctcaacgca aacgcaacac tccagccgta tttcgcggcc agccacgggg
    41461 tggcgatcat agagaagaaa gagccgatgt tgacggacat gtagtacatg gtgaatgcac
    41521 cgtccagacg cgggtcgttt ttctcatagc atgtagaaag cagagaagac gggttagctt
    41581 taaacaggcc gttaccgacc gcaatagccg ccatacccat ataaacgata ccggcgtcgt
    41641 gaccagacca ggcaaccaga gcataaccaa tcgccagcac aatagcgcca agcataatta
    41701 cgcgtttagt acccagtacc ttgtcaccta accagccgcc gatagcgacc agaccataaa
    41761 ccagggcact aaaggaagag aaaagggtga ttgaatccgc ttcagacata cccagttgtt
    41821 taaccaggta aacagccata attccttgta ggccgtaata accaaaacgt tcccataact
    41881 caatcgagaa gatgagatag aacgccttcg gttgtttgaa agcgttcaaa ctgacgcttt
    41941 cagttggttt ttggtttgca gtggacactt ttacctcttt ttttacatcc catattaacg
    42001 ggggtgcttt ctcgcgttat gcctaaaaaa catacgcgca attgttatag tgggagggga
    42061 aacggcaggt aatgttcact atcttgcact ttcagacaag tcctttgtaa taatctgtta
    42121 catataactg aggcggggaa ttcaaccatt ccgggaaatg ttatccagcg ttaaattttt
    42181 caaaaaaacg taacggcaga tttttttcac taccctgaga gggggatgat ggcttaacgg
    42241 cgatatgttc tgctatttag cgtttttcct agtgagatag tccagtttct gaaaaatagc
    42301 cagtgtaatg ttttgtaggt caatatttg
//
LOCUS       DAZUCC010000015        41531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZUCC010000015 DAZUCC010000000
VERSION     DAZUCC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.015"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..335
                     /locus_tag="ACHY09_000714"
     CDS             <1..335
                     /locus_tag="ACHY09_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3883728.1"
                     /translation="KKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKPFS
                     TIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSNIDLAKRRYKEL
                     VQERKNEN"
     gene            325..615
                     /locus_tag="ACHY09_000715"
     CDS             325..615
                     /locus_tag="ACHY09_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3883729.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(668..2521)
                     /gene="asmA"
                     /locus_tag="ACHY09_000716"
     CDS             complement(668..2521)
                     /gene="asmA"
                     /locus_tag="ACHY09_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFS3883730.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQVNWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLKETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVIGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(2543..3124)
                     /gene="dcd"
                     /locus_tag="ACHY09_000717"
     CDS             complement(2543..3124)
                     /gene="dcd"
                     /locus_tag="ACHY09_000717"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFS3883731.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(3216..3857)
                     /gene="udk"
                     /locus_tag="ACHY09_000718"
     CDS             complement(3216..3857)
                     /gene="udk"
                     /locus_tag="ACHY09_000718"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS3883732.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            4175..7492
                     /gene="dgcE"
                     /locus_tag="ACHY09_000719"
     CDS             4175..7492
                     /gene="dgcE"
                     /locus_tag="ACHY09_000719"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS3883733.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNMADWLRLALGSAIVPPLLGGVLVILLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(7601..8449)
                     /gene="alkA"
                     /locus_tag="ACHY09_000720"
     CDS             complement(7601..8449)
                     /gene="alkA"
                     /locus_tag="ACHY09_000720"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFS3883734.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEC
                     RGVVTAIPDIASHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPLRSYALLHIWYTEG
                     WQPDEA"
     gene            8583..9935
                     /gene="yegD"
                     /locus_tag="ACHY09_000721"
     CDS             8583..9935
                     /gene="yegD"
                     /locus_tag="ACHY09_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3883735.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(9948..11887)
                     /gene="yegI"
                     /locus_tag="ACHY09_000722"
                     /pseudo
     CDS             complement(9948..11887)
                     /gene="yegI"
                     /locus_tag="ACHY09_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(11884..12645)
                     /gene="pphC"
                     /locus_tag="ACHY09_000723"
     CDS             complement(11884..12645)
                     /gene="pphC"
                     /locus_tag="ACHY09_000723"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFS3883736.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSGPAVNERTDDDKTLALALWLP"
     gene            complement(12642..13301)
                     /gene="yegL"
                     /locus_tag="ACHY09_000724"
     CDS             complement(12642..13301)
                     /gene="yegL"
                     /locus_tag="ACHY09_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFS3883737.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(13522..13581)
                     /gene="ibsA"
                     /locus_tag="ACHY09_000725"
     CDS             complement(13522..13581)
                     /gene="ibsA"
                     /locus_tag="ACHY09_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFS3883738.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(13854..13910)
                     /locus_tag="ACHY09_000726"
     CDS             complement(13854..13910)
                     /locus_tag="ACHY09_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS3883739.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(14182..14238)
                     /locus_tag="ACHY09_000727"
     CDS             complement(14182..14238)
                     /locus_tag="ACHY09_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS3883740.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(14510..14566)
                     /locus_tag="ACHY09_000728"
     CDS             complement(14510..14566)
                     /locus_tag="ACHY09_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS3883741.1"
                     /translation="MMKILIIVVLLVVSYPAY"
     gene            14845..16092
                     /gene="mdtA"
                     /locus_tag="ACHY09_000729"
     CDS             14845..16092
                     /gene="mdtA"
                     /locus_tag="ACHY09_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFS3883742.1"
                     /translation="MKSSYKSRWVMVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            16092..19214
                     /gene="mdtB"
                     /locus_tag="ACHY09_000730"
     CDS             16092..19214
                     /gene="mdtB"
                     /locus_tag="ACHY09_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFS3883743.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGQGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            19215..22292
                     /gene="mdtC"
                     /locus_tag="ACHY09_000731"
     CDS             19215..22292
                     /gene="mdtC"
                     /locus_tag="ACHY09_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFS3883744.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRIGLNPQALFNQGVSLDDVRSAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGALRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            22293..23708
                     /gene="mdtD"
                     /locus_tag="ACHY09_000732"
     CDS             22293..23708
                     /gene="mdtD"
                     /locus_tag="ACHY09_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3883745.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQIGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAITTLMLMPNYTMQTRCFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNCALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSAQ"
     gene            23705..25108
                     /gene="baeS"
                     /locus_tag="ACHY09_000733"
     CDS             23705..25108
                     /gene="baeS"
                     /locus_tag="ACHY09_000733"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFS3883746.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            25105..25827
                     /gene="baeR"
                     /locus_tag="ACHY09_000734"
     CDS             25105..25827
                     /gene="baeR"
                     /locus_tag="ACHY09_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFS3883747.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLMIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLEALDEE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26018..26350
                     /gene="yegP"
                     /locus_tag="ACHY09_000735"
     CDS             26018..26350
                     /gene="yegP"
                     /locus_tag="ACHY09_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFS3883748.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            26498..27859
                     /gene="yegQ"
                     /locus_tag="ACHY09_000736"
     CDS             26498..27859
                     /gene="yegQ"
                     /locus_tag="ACHY09_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFS3883749.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTTFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28132..28350)
                     /gene="ogrK"
                     /locus_tag="ACHY09_000737"
     CDS             complement(28132..28350)
                     /gene="ogrK"
                     /locus_tag="ACHY09_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFS3883750.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(28432..29595)
                     /locus_tag="ACHY09_000738"
     CDS             complement(28432..29595)
                     /locus_tag="ACHY09_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFS3883751.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDISGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQVTITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(29595..30074)
                     /locus_tag="ACHY09_000739"
     CDS             complement(29595..30074)
                     /locus_tag="ACHY09_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3883752.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATRFTGMTGAG"
     gene            complement(30089..32536)
                     /locus_tag="ACHY09_000740"
     CDS             complement(30089..32536)
                     /locus_tag="ACHY09_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS3883753.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAETMAGVKLLMSGYEFAQK
                     NSELQAVLGVAKDSAEMTALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTVASTGKLDKLTAAFKASDGKTSELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIAGGALVLTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFTNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(32529..32684)
                     /locus_tag="ACHY09_000741"
     CDS             complement(32529..32684)
                     /locus_tag="ACHY09_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFS3883754.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGSTNE"
     gene            complement(32681..32956)
                     /locus_tag="ACHY09_000742"
     CDS             complement(32681..32956)
                     /locus_tag="ACHY09_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFS3883755.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(33013..33531)
                     /locus_tag="ACHY09_000743"
     CDS             complement(33013..33531)
                     /locus_tag="ACHY09_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFS3883756.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATSADAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEVDTGEGKQGEDTESKLPVICTYFKLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(33544..34734)
                     /locus_tag="ACHY09_000744"
     CDS             complement(33544..34734)
                     /locus_tag="ACHY09_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFS3883757.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALMGAESVTGVKPRILGVPGLDTKEVAVALASVCQELNAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQKPGTDADLLNEAGVTTLVRRDGFRFWGN
                     RTCSDDPLFLFESYTRTAQVLADTMAEAHMWAIDKPITATLIRDIIDGINAKFRELKN
                     NGYIVDGTCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            35147..35590
                     /locus_tag="ACHY09_000745"
     CDS             35147..35590
                     /locus_tag="ACHY09_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS3883758.1"
                     /translation="MLDIRMSRPSEASEIIQIWKSSVDATHDFLTGHDRQEIEKEVIG
                     FFSETPVWVATNQDDQPLGFMFLHEGHLEALFVAGSARGLGVGKRLISHALALHPELS
                     VDVNEQNQQAVGFYQHMGFQVSGRSDLDNQGRPYPLLHLRMANNM"
     gene            complement(35611..36195)
                     /locus_tag="ACHY09_000746"
     CDS             complement(35611..36195)
                     /locus_tag="ACHY09_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS3883759.1"
                     /translation="MDKKFKNQIVDSDIARLLLKHRKARNLTVTELAQRSGVSQAMIS
                     KIERGISSPSATILSRLANAMNVTLSKLFAELEQHQNSLVRFAHQQQHWTDEQSGITR
                     WSLSPAGACPELIKVEIPAMGELTIPASANEHLSGQTLWMMSGSLDFRVKNQTHHLRA
                     GDCLALTFASEYQMSNPDSEQSCSYIVAFSQKNQ"
     gene            complement(36431..36958)
                     /locus_tag="ACHY09_000747"
     CDS             complement(36431..36958)
                     /locus_tag="ACHY09_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS3883760.1"
                     /translation="MQHLKNITAGNPKTVAQYQLTKNFDVIWLWSEEGKNWYEEVSNF
                     QEDTIKIVYDENNIIVGITRDASTLNPEGFSVVEVPDITSNRRADDSGKWMFKDGAVI
                     KRIYTADEQEQQAESQKAALLSEAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(36962..39007)
                     /locus_tag="ACHY09_000748"
     CDS             complement(36962..39007)
                     /locus_tag="ACHY09_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3883761.1"
                     /translation="MSTKFKTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSMSETLAATPKAVKTVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQ
                     IANTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTA
                     LAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNRAGNAV
                     QKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSYMWFETGDNGNEYFKW
                     RSRQSTTTKDLMNLKWDALYVLVKALFSSEVKISTVNALRIFNSSFGAIFRRSEENLY
                     IIPTRENEGENGDIGSLRPFGINLRTGVVSVGNGARIDGGLALGTNNALGGNSIVLGD
                     NDTGFKQNGDGNLDVYANNVHVMRFVSGSIQSNKTINITGRVNPSDYGNFDSRYVRDI
                     RLGTRVVQTMQKGVMYEKAGHVITGLGIVGEVDGDDPAVFRPIQKYINGTWYNVAQV"
     gene            complement(39018..39548)
                     /locus_tag="ACHY09_000749"
     CDS             complement(39018..39548)
                     /locus_tag="ACHY09_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFS3883762.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDSCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWETSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(39541..40449)
                     /locus_tag="ACHY09_000750"
     CDS             complement(39541..40449)
                     /locus_tag="ACHY09_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFS3883763.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELLWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(40454..40801)
                     /locus_tag="ACHY09_000751"
     CDS             complement(40454..40801)
                     /locus_tag="ACHY09_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFS3883764.1"
                     /translation="MTARYLGMNRSNGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(40798..41433)
                     /locus_tag="ACHY09_000752"
     CDS             complement(40798..41433)
                     /locus_tag="ACHY09_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFS3883765.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITSTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
BASE COUNT         9408 a        11270 c        10834 g        10019 t
ORIGIN      
        1 gaaaaaagct ggcttttctt gataccagtc ttgacgattt acgcgcattt ccagaatcgt
       61 ctcgacagga aattggctat caactcgaca ggatccaaca agggttaaac ccatacgact
      121 ggaaaccttt ttcaactatc ggccctggtg tgcgagagat tcgtactcgc gatgctgatg
      181 ggatctatcg cgtcatgtat gtcgcaaaat ttgaagaagc ggtttacgta ttgcattgtt
      241 ttcaaaagaa aacacagact accagtcaat cgaatattga tttagccaaa cgacgctata
      301 aagaacttgt tcaggagcgg aaaaatgaaa attgaaacct ttgatagtgt gtgggatgcg
      361 gtgagtgaca cccctgaaca agcggaaaat atgcggatcc gtgccgaact tgttacgatc
      421 attaataact ggattgaaca acaagggttt agccaggctc aggcagcgtc tgctttaggg
      481 gtcactcaac ctcgcatatc tgaacttgcc agaggtaaga tccagatctt tagtatcgat
      541 aaattgatca ccatgatggc tcatgcagga ttgcatattc aacgtattga agttcaatat
      601 ccacatgctg cttagtacta taaatacaga tggcgacaaa ttgacgccgc catctgcata
      661 acttgtctta catcttctcc agcaacttct tcacatcttt gccattgcgg gaatctttat
      721 tccgctcggc ccagtcattc aggcgacgtt tcgcttcgtc ctgtagatgt ttgcgcagta
      781 actgatccac ttgcaggctg taattaagtt gctgccagtt gccataaacc cgcagcggta
      841 ctggcgtttc tttgaggaaa tcaatcagtt tgctttcccc gttccaaccg ccaatcacac
      901 gaatgtcaaa ctgggtgtcg caggtttgat ctgccagatt caacatgcct tcccctgtca
      961 gcgccagcac aggcgattga ccttgcatgt cgtttaacgt cacgacgcca tccttcaacg
     1021 tcaaatcggt ggtaaagcgg tcaagacgcg ttacgttatc gaagttttca gcggccttca
     1081 catcaccacc attacgttct accgcttgct gaatcatctg ctggaagttc atcccttcca
     1141 tgcgcgtgtc ggtcatttcg acatgtgcct gtccttgcca gttatggcgg aatgcgtcgg
     1201 catctatgtc agcaccggag aaatcaccag ccagtgacat ctttccggtc aacgaaatcg
     1261 gatagttaaa cgccttcaga atggtgccaa tctcaacgtt ttccagccgt ggctggaagt
     1321 ttattcgcgg atttattgat gtcgcgtcca gcgtgcccgg cagtgaaacc tgtccaccgt
     1381 taagtttgcc ctgcagttga gtaatttcca gcagacccga cttgttggtc atttgcgtgg
     1441 caacatctgt aaaattcatt ccgcgccagc gcacgttact ggcctgcaac aaaatatcag
     1501 ccgtaaagcc ttgcagtccc tgataggccg gttcatcaat acgcgaagaa atgaccgggc
     1561 gcggcaacgt ggattggctc tgcccctgct gcgcggcacc gttttcaccg ttcgctgttt
     1621 ctttaagcgg gatgaggttg tcaagattca gttgcgggaa ttgcagcctc agctgccatt
     1681 ccggtttctc tgtcagcgtg acctgtgctt gcccgctcag cgtactgtca ttggcggtca
     1741 agctgatttg gttaaatgaa aggcgtttat gtgactcctg ccactgggcc tggaaactcc
     1801 cctgcccctg aataccttgt tttggtaaat ctgcaccctg caactgccag ttaacttgtt
     1861 caatagccgc cgttaaatca tgcggataat cagaagcatc taccgtaccg ttaagggata
     1921 tcgtgagatc gcgctgatcg cgattaaccc gcccggagaa ctcaaatgaa ccacgatgtt
     1981 ggggatcttg ttccatttgc aggcgaatat tgcggattgt cacctgctcg tcatcttcat
     2041 gctggaacac cagcacgctg tccgccacct taagactgga tatatcaaac gaccatccgc
     2101 gatcgtctga cagatccggc aaggtattgt cgcgcggtgc aaccggagcg tcttcactgc
     2161 gcaccgcttc cgtctgcggc gtcagttgga tcactgcccc ttttagcatc acctgcttaa
     2221 cgctcagttg atgactcagc agtggtaaaa gcgccacgtc cagacgcatg ttgtctgcgc
     2281 gaaccagtgg ctggctcgcg ccctgggcag tgagagacat tcgcccggag aggatactaa
     2341 gctgcggcca gacgtgccaa cgcagtggcc cgtcgagctg caattgataa ccgctacgtg
     2401 cagcaacttg cttgaccata tagtcgcgga aatcattcgg attcaccagc aacactaacg
     2461 cagataaccc ggccaccagc acgaccagga gtatcatcag cgtcgtcaga aatcgtctca
     2521 tggtatcctc aatgggcctg aattagtctt tatcgattcg actggctacc gcgccctgct
     2581 ggttgcgata tttcgcatct tcacggcggt tgtaaggtcg cgccgccggg ccggaaagcg
     2641 gctcaaagct cagcgcacca attaacatgc ccggacgcag cgccagcggc agcttaccgg
     2701 agttgtagaa ctccagcaca atacagccag accagcccgg atcgatacgg tgcgcggtga
     2761 cgtgcaccat aagccccaga cgcgccagtg aggaacgacc gtccagccat cccaccagat
     2821 cggctggcag cgtcaccgac tccagcgtca ccgccagcgc cagctctcct gggtgaagat
     2881 aaaacgcctc gctttcatcg agtacgatct catcgctcat cacgcggtca agcgcggcgc
     2941 tcacttcatc tttgggaccg ctcagatcga taaacgcagc cgtgtgacca cggaaggtac
     3001 gaaatttatt gcccaggcgc acatccaccg tcgcgccgtt aatacgctcc actggcggac
     3061 gtgggttgat cgacaaacgg ccttcatcaa gccaggcttc aatatctcgg tcacacagac
     3121 gcatggcatt ttctcctttc gcgcatcact cccttaacgc caattacgtc aagggcatac
     3181 caggttatca ctgaacggta cacaatttat caagcttatt caaagaactg acttattttc
     3241 gctttcaata tatcgatggc gatgcggttt ttcccgccgc gcggcacgat aatgtccgca
     3301 tattgtttag aaggctcaat gaattgcagg aacatcggac gcacggtttt ttgatattgc
     3361 gccatcactg aatccattga acgcccacgc tcgttaacgt cacgcttgat gcggcgcatc
     3421 aggcagatat ccagcggggt atcaacgaaa atggagaagt taagttcatc acgcaaacgc
     3481 gcatccgtca gcagcaaaat gccttcgaga atgatgactt tcttcggctc aaccgtcacc
     3541 gtttctttca tacgcgtatg ttcaacatag ctgtaaaccg gcaggtcaat ggccgagccg
     3601 cgtttcaacg cttgtaaatg ctcaagcaac aggctgtgat ccatagcgct gggatggtcg
     3661 tagttggtct taacgcgttc ttccatcgac agatggcttt gatctttgta atagcagtct
     3721 tcgggaatta cgccgatgtg ttcatcaccg acttgctcac gtaattcacg ataaagggta
     3781 ctggcaataa gactcttgcc ggaagccgat gcgccagcga taccgataat gacgcactga
     3841 tgagactgat cagtcatata tttagcgacc tgattaacct ggatgttagg aagggggcga
     3901 cgaagcgcca aacgcggcaa ttatagggat ttcatccgcc tgataccagt cgaatagcgt
     3961 tgccgcgcgc tcagagttaa ttgttgacga agaattcccg gtggcaaatt acgttgatca
     4021 gttttataca aggtaaaaat tgttatacgc agttgcgcaa attatccgcc tttacgtcac
     4081 tttatgagca attcgcatat aaaatgtaaa acttttgtac tagcataaac acagaaacga
     4141 atactggcga cctggtcttg cggataaagc ggtaatgagc aaacaatcac agcatgtatt
     4201 aattgccctg ccccacccgc tgcttcacct ggtcagttta ggtttagtct cgtttatctt
     4261 tacccttttc tcgcttgagc tttcgcagtt tggcacccaa ctcgccccac tgtggttccc
     4321 gacgtccatc atgatggtgg cgttttatcg ccatgccggg cgcatgtggc cggggattgc
     4381 gctgagctgc tcgctgggaa atatcgccgc atccatcctg cttttttcca ccagctcgct
     4441 gaacatgacc tggacgacca tcaatattgt tgaagccgtg gtcggggcag tgctactgcg
     4501 taaattgctg ccgtggtata acccattgca aaatatggct gactggctgc gtctggcact
     4561 cggcagcgcc attgttccac ctctgttagg gggtgttctg gttatcctgc tgacgcccgg
     4621 agacgatcct ctcagggcat ttttgatatg ggtactgtca gaatccatcg gcgcactggc
     4681 actggtgccg ctgggattgt tatttaaacc acactatctg ctgcgccatc gcaacccacg
     4741 gttgcttttt gagtcgctgc tcacgttagc catcacactg acgttaagct ggctttcgat
     4801 gctgtatctg ccgtggcctt ttactttcat tattgtgctg ttgatgtgga gcgccgtgcg
     4861 cctgccacga atggaagcct ttttgatctt ccttaccacg gtgatgatgg tgtcactgat
     4921 gatggccgcg gatccctccc tgcttgctac gccgcgtaca tacctgatga gccatatgcc
     4981 gtggctaccg tttttgctga tcctgctgcc cgccaacatc atgaccatgg tgatgtatgc
     5041 ctttcgtgcg gaacgcaaac acatttccga aagcgaaacc cgctttcgga acgcgatgga
     5101 atattccgcc atcggcatgg cattagtggg caccgaggga caatggctgc aatccaacaa
     5161 agcgctctgc cagtttctcg gttacagtca ggaagagctg cgcggactca cctttcagca
     5221 actgacctgg ccggaggatc tcaataaaga tctgcaacag gttgaaaagc tgatcagcgg
     5281 tgaaataaac acctattcaa tggaaaaacg ctactacaac cgcaatggcg atgttgtctg
     5341 ggcgttgctt gccgtctcac tggtgcgcca caccgatggt acgccgctct attttatcgc
     5401 tcagattgaa gacattaacg agctaaaacg caccgaacag gtgaaccagc aactgatgga
     5461 gcgcatcacg ctggctaacg aagcgggcgg gattggcatc tgggagtggg agctaaagcc
     5521 gaatattttt agctgggata agcggatgtt cgagctgtat gaaattcctc cgcatatcaa
     5581 accgaactgg caggtgtggt acgagtgcgt actgccggaa gatcgccagc atgccgaaaa
     5641 agtgattcgt gattcgttgc aatcacgctc gccctttaag ctggaatttc gcattgccgt
     5701 gaaagatggc atccgccata tccgcgccct cgccaaccgg gtactgaata aagaaggcga
     5761 agtcgaacgc ctgctcggca ttaatatgga tatgaccgaa gtgaaacagc ttaacgaggc
     5821 attgtttcag gaaaaagagc gcctgcacat tacgctggat tccatcggcg aagccgtggt
     5881 ctgtattgat atggcaatga aaattacctt tatgaatccg gtggcggaga agatgagcgg
     5941 ctggacgcag gaagaagcgt taggtgttcc gctcctgacg gtgttgcata ttacttttgg
     6001 cgacaacgga ccattaatgg agaacattta cagtgccgac acctcacgtt ccgcgattga
     6061 acaagatgtg gtgttgcact gtcggagcgg cggcagctac gacgtgcatt acagtattac
     6121 gccgttaagt actctggacg gcagcaatat aggctcggtt ctggtgattc aggacgtcac
     6181 cgaatcgcgt aaaatgctgc gccagctgag ctacagcgcc tcccatgatg cactgacgca
     6241 tctcgccaat cgcgccagtt ttgagaagca actacgcatc ctgctgcaaa cggtaaacag
     6301 tacacatcag cgacatgcac tggtgtttat cgatcttgat cgctttaaag cggtgaatga
     6361 cagcgccggg cacgccgctg gcgacgcttt actgcgcgaa ctggcgtcat tgatgctgag
     6421 tatgctgcgc tccagcgacg tgctggcgcg actcggtggc gatgaatttg gtctgctgct
     6481 gccagattgt aatgtcgaaa gtgcgcgttt tatcgctaca cgcattatca gcgccgtgaa
     6541 tgactatcac tttatctggg aaggacgtgt acatcgggta ggtgccagtg ccgggattac
     6601 cttgattgat gacaacaatc atcaggcggc tgaagtgatg tcgcaggctg atatcgcctg
     6661 ttatgcctcc aaaaatggtg gacggggccg ggtgacggtt tacgaaccgc agcaagctgc
     6721 cgcacatagc gaacgggcgg tgatgtcgct tgatgaacag tggcggatga ttaaagagaa
     6781 tcagttgatg atgatcgccc acggtgtcgc ttcgccgcgg atcccggaag cgcgtaattt
     6841 gtggctgatt tcacttaagc tctggagttg cgaaggcgag attattgatg aacaaacatt
     6901 tcgtcgtagc ttcagcgatc cggcacttag ccatgctctt gaccgacggg tattccacga
     6961 ttttttccag caggccgcaa aagcgattgc cagtaaaggc ttaagcatcg ccctccccct
     7021 ttccgttgcc ggtttgagta gcgccacgct ggtgaatgaa ctgcttgagc agctggaaaa
     7081 tagccctcta cccccacggt tattacatct gattattccg gcagacgcga ttttagatca
     7141 cgcagaaagc gtgcaaaaac tgcggctggc gggatgtcgg atcgtattca gtcaggtggg
     7201 ccgcgatctg caaatcttca actcgctgaa agcgaatatg gcagattacc tgctacttga
     7261 tggtgagtta tgcgccaatg tgcagggaaa tttgatggat gagatgctga ttacgatcat
     7321 tcaggggcac gctcagcggc tcgggatgaa gaccatcgcc gggccagtag ttttacccct
     7381 ggtgatggat acgctttctg gcatcggcgt cgatctgatt tatggcgatg tgattgccga
     7441 tgcccaaccg ctggatttgc tggtgaatag cagttatttc gcgattaact gaggcgtgct
     7501 tctccatcgc ctgatacgac gctaacgcgt cttatcatgc ctacaaatcg ctcattcccc
     7561 aggccggata aggcgctcgc accgcatccg gcgaccaacg tcatgcttcg tctggttgcc
     7621 agccttccgt ataccagata tgcaacagcg cataagaacg caagggcttc cagcgttcgg
     7681 cataacggcg gatttgtgcc ggtgtcattc ccggaaatcg ctgtttaatc agataatcat
     7741 ccggcaggaa aacatctttc gcctgccagc cgcgcaaggc gaaataattc gccgtccagc
     7801 gcccgatacc cggaaaagtt tgcagcgttt tcatcgcctg ctccacatcg cccggtattg
     7861 tcattggtaa ggtgccctcc agcgccgcat ttgccagatg aatcagcgcc tctgcccgtt
     7921 tcaacggcat acctaacgct tttaatgcct gcggatctgc tgctgccagc cgctgaggcg
     7981 tggggaagca gacataatcc ggaaaatcat ccagcctttc gccataaagc tgtgccactc
     8041 tggcggtcaa ttttgccgcc atcgccacgc tcaccagctg gcctaaaatc gcccgcacgc
     8101 cctgctcaaa agcatcaaca caaccgggta aacgcaatcc gggccgtgcc gcgcctaact
     8161 tgcccaacgc accgttaaca atctgcgggt tacattgcag atcaaacagg cggctcattt
     8221 tcgccagaca ctctgcggca acgggttcta aacctgcact taaatttatg tgcagagtat
     8281 ggctggctat atccggaata gcagtcacca cgccgcgaca ttcgcccacc gccagactac
     8341 gggcataata atcgtcagcg accgtttcca caccgttcac cgcacgggcg gcgagaaatc
     8401 ccaacatcca cgaccagtca tacggcggct gccagttcag ggtatacatc gcatctcctt
     8461 gttaatccgc tttcagcata aacgttattc agacgctacg ctttgctttc atattccggt
     8521 tgtcgcgacg gcaacatttc gctaaagtca cgccccttct tcaccggcat ggggattatt
     8581 tcgtgtttat tggttttgat tacggtacag caaactgttc agtggcggtc atgcgcgacg
     8641 gtaagccgca attactgaaa atggaaaacg acagcacgct gctgccttca atgctttgcg
     8701 cgccaacgcg tgaagcggta agcgaatggc tgtaccgcca tcatgatgtt ccggcagacg
     8761 acgatgaaac gcaggcgctg ttgcgtcggg cgattcgtta taaccgcgaa gaagatatcg
     8821 atgttacggc gaaaagcgtg cagttcggtc tttcctcact ggcacagtac attgatgatc
     8881 cagaagaggt gtggtttgtg aaatcaccaa aatcattcct cggtgccagc ggcttaaaac
     8941 cgcagcaggt agcgctgttt gaggatctgg tctgcgcaat gatgttgcac attcgccagc
     9001 aggcgcaggc gcagctgccg gaggcaatta ctcaggcggt gattggtcgc ccgatcaact
     9061 tccaggggct gggcggcgat gaagcaaacg cccaggcgca agggattctg gaacgcgcgg
     9121 cgaagcgtgc cgggtttaag gatgtggtat tccagtacga gccggtcgcg gctgggctgg
     9181 attacgaagc caccttgcag gaagaaaaac gggtgctggt ggtggatatc ggcggtggta
     9241 cgactgactg ttcattgctg ctgatggggc cgcagtggcg ttcgcgtctc gatcgtgaag
     9301 ccagcctgct gggtcacagt ggttgccgta ttggcggtaa cgatctggat atcgcactgg
     9361 cgtttaaaaa cctgatgccg ttactgggca tgggtggcga aaccgaaaaa ggcatcgccc
     9421 tgccgattct gccgtggtgg aatgcggttg ccatcaacga cgttcctgcg caaagtgatt
     9481 tctacagtag tgccaacggt cgtctgctta acgacctggt acgcgatgcc cgcgaaccgg
     9541 aaaaagtggc cctgttacag aaagtctggc gtcagcgttt aagctatcgc ctggtacgta
     9601 gcgcagaaga gagcaaaatt gcgctttcaa gcgtagcgga aacccgcgcc tcactgccgt
     9661 ttatcagcga tgaactggct acgctgatta gccagcaagg gctggaaagc gccctcagcc
     9721 agccactggc gcggattctg gaacaggtgc aactggcgct ggataacgcc caggaaaaac
     9781 cggatgttat ctatctgacc ggcggtagcg cccgttcacc gctgattaaa aaagcgctga
     9841 cagaacagtt gccgggcatt ccgattgcag gtggcgatga ctttggctcc gtcaccgccg
     9901 ggctggcacg ctgggcggaa gtggtgtttc gttaattgaa aactggctca gcaacggctt
     9961 aaatcagcct gcgcctgagc cagtttttct gccgcctgac gtagcggttc catcaacggc
    10021 atggtccgtg ctggagcatg aaggcggaat cgctgcaact ctgtcgcacc gacattcagc
    10081 gccgattcca gccgatgtcg tttggcggcc atttttgcca ttaccgcttg tctgtctgca
    10141 ggcgttaccg cttcgttcgg cctgaacaca aaacggcgtt cacagctcgc tttccagtcg
    10201 ataaccgcct gggtcagatg atcaccaaaa cctttcactt gcttaacgct ccgtcgggta
    10261 acatccgctg ctgtttcaat accaaaggac cgtaacgccg ctttacgcgc agggccaacg
    10321 ccgggaatag aggcaacatc aataaaaaat ccctccagaa acttctgctt ctgccgttcc
    10381 cttgcggtgt cctgaagtgc tgccagatcg cgcttttctt cttccggtaa cccaagaatt
    10441 tcgtccttca ttttttcgag catcgtccgt ttggcgataa aaccttccag cccgcccaac
    10501 tgctggatct ggctgactaa atggtcatag tccattttcg cgcgattaaa cgcctcacgg
    10561 cgttgctgga tttctgcttt gtacgctttg cttgtcaggc tgccaataat ccagatagcc
    10621 gccagcacag gaaccaaaat aatataacgt ggttctgtct gaaggccgca aagcagcagc
    10681 gataacgctg acagtgcgat ctcaatcaga atgatgtatt cacgccttaa caggcctaaa
    10741 ggaagcggcc tgccagtcgg ttggaaatga tcgggtaatg gtaattgcaa tgctggcggt
    10801 gctactgacg ccatcaccat cgcccagact ttcgccagca caaaatcacc gctggtggta
    10861 atgacctctt cgccgagatc aataaaatag ataacgcctt gattatccag agcacaccac
    10921 gggcagtcag ccaaatgacc gggataaaca tgcattgccg aaacggtaca tttctttaac
    10981 tgttggcgta gtaaatcaag tgccgctacc cacgcttttg ccgtcggacg cccggttgcc
    11041 acgccacttt ccgtgaatgc ctgctgaaac atggcttcaa catgcccggt aacatcgaca
    11101 gcggaataga tcgtggcggc ggttttaaac cacgtcgctg attatctgac gcgtaggcat
    11161 aacggaaatg ggcaatatcc gtctccagtg cattacccgc atcagagaca agcggtacac
    11221 cggaataagg atgccgccca ccaaacaaga cgtgaaaaat cagcaacgca aggccaaaat
    11281 tatcgtgatt cgcggtgcgt tcaaagccga caaatgatgg caaggtttgt tgctctggcg
    11341 gcgtaaaatg cgacacgccg acttcgcata aatgcagcgt gccattggcg ttaatctgaa
    11401 aggagtcgct atcaatcaac accactttgc tgtcgcgacc taccataaag ctgttctggt
    11461 ttacgtcacc cacgacgtgc ccgtgctcgt gaatcgtagc aaaagatgaa gcaatattgc
    11521 gcgcaacata gagtagaaaa tcccacgcac aatgagggta acgttgacga cgatgtgccg
    11581 ggctatagat catatgaatc ggttctttac cagaaacttt tggcatcata aaaccgatta
    11641 cttttccgcc tcgtccaccg tgaagcgttg cctgcggcca ggcgacataa ttcaacaact
    11701 gcgcgtcagc tgtcgcagcc ataaaggcaa gtttgtcctg ttttaaggcg ggtggcggcg
    11761 tgtgataaat cttggcgacg ctatcgacaa actcctcgat atcataaacc gcgccttcac
    11821 cgcctttgcc cagttcacag cctggcgtaa cgcactcacc agtagcagta tataaagtgg
    11881 gtttcatggt aaccacagcg ccagtgccaa cgttttatca tcatcggtac gctcgtttac
    11941 cgccgggccg gacaaaaact gctttaacaa ttcaggtaat aaatcgagtt gttcctgcgt
    12001 tgctgacgcc agcccattga aaaacggggt aaagaaaggc acatgcggag aattatccag
    12061 catatttaac gccagccgct ggataccatc cgtaaatgct gcaactttgt gcgcacgctc
    12121 agtgctggtg aaagtttcca gtctggaaac ggcatcttca tcggtaatga agtgcgtcat
    12181 attggtatat tcacccacca tcggcgtgag cggcagttgc aaaccgtgcc ccaaatctac
    12241 caccacgccg ccatcgccaa tctgcataat caaggtgcca ttagctgatg aaatcaatcc
    12301 caaaaacgtg caggcaaaat cgcgaaccgc taattctttg gcttccgcct cggcaaaaag
    12361 tcgctgacga atggtcagca caatgtccgt tgcgagaaca tcattaagcc ccaattcccc
    12421 gccctgcact ttttgcgaca tataagccat cgcttcattg acggcgagca tcgcaccttc
    12481 gcctccctgc gaggcgctac ctgcgccatc agcgacaaat accgacaata atggctgctg
    12541 gtcatttaac caggcaacct gcatctggca ggcatcctgg caaggcaagt ccgcgctgat
    12601 gtgcgacgtt ccaacggttg atgcatagac cagacgccag ctcacactga cgtccagcct
    12661 tttggcgctt ccagcacaac ttccgttccc ggcgtggagc gggaaaccga acgcagcgaa
    12721 ctggataacc agctaaatag ttcgcggaat tgtaatcctt gaagaggcaa aggctgacga
    12781 acgctgattt gggccagcgt cttcatatcc gccccctgaa caccgatgga aaagaaggca
    12841 aacttcttat cttcttcccc ctgaaacacc ttgttggcag cagcctgcca ctcatcggtt
    12901 ggtgcgccat cggtaatcag gaaaatccac ggacgataat aggaaatacc attggcgcga
    12961 tactcacgtt tgcgctcctc aaccatatcc agggctttgg taatggcggc ccccattggt
    13021 gtatcgccct gggcaaacag gatgggcggg aaaaaattag cggcgctggt aaatggttgt
    13081 tccacatgca ccgggccgaa cgtcacaatg ccgagttcca ctctttttaa ggccagcgga
    13141 tcggcaagca gctcatcgcg aaaggtaacc aatccggcgt taagttcatt aataggtcta
    13201 ccgttcatag aaccagaaac atccaacagc aaaatgcagg ggcaacgtgg ttccgggtta
    13261 ctggcaaaat cgctggtggc aaacgtgatt tgttcgctca taagaaatcc tttcttagag
    13321 aattattgag gcccggtatc ataccgactg caacaatgaa ataagagcaa acgaagaaac
    13381 tttcagaatt aggcccggcg gcgcagaaaa ttttgctatt cgcctcgcaa gaacggagag
    13441 ccgtcgattg acactaaggg cggagtgaca taatttcagg agtgagggtt agggagaggt
    13501 ttccccctcc ccctggtgtt cttagtaagc ctggaagcta atcactaaga gtatcaccag
    13561 tatgatgacg tgcttcatca taaccctttc cttattaaaa gccctcttct ccgggagagg
    13621 gtttcccgtt tcagcgtccc gctgaaatcg tcggcttacc tcctttcgcc ctgaatgcag
    13681 tctatcgcta acgcgtgaac aaaatagttt cctctgtgat tattgcgtaa cgactcgcaa
    13741 agaatatttg ctggcaggat cgcagactac aaagcctgcg gattgacaat cttatcgtga
    13801 aggcataatt tcaggagtga gggtagagcg gggtttcccc cgccctggta gtcttagtaa
    13861 gctggccagc taataactaa gagcaccacg atgatgagta gcttcatcat gaccctttcc
    13921 ttatttaagg ccccttcctc gggaggggct ttcccgtttc agcgtcccgc tgaaatcggc
    13981 ggcctacctc ctttcgccat gcaagcagtc tatcgctaac ttgcagataa aatagtttca
    14041 catgtgatta ttgcgtaacg tctcgcaaag aatatttgct ggcaggatcg cagactacaa
    14101 agcctgcgga ttgacaatct aattgtgaag gcatactttc aggagtgagg gtagagcggg
    14161 gtttcccccg ccctggtagt cttagtaagc cgggaagctt atgactaaga gcaccacgat
    14221 gatgatccac ttcatcatga ccctttcctt atttaaggcc ccttcctcgg gaggggcttt
    14281 cccgtttcag cgtcccgctg aaatcatcgg cttacctcct ttcgccctga atgcagtcta
    14341 tcgctgactt gcggataaaa tagtttcaca tgtgattatt gcgtgacgtc tcgcaaagaa
    14401 tatttgctgg caggatcgca gactgcaaag cctgcggatt gacaatcgga ccgtgaaggc
    14461 atactttcag gagtgagggt agagcggggt ttcccccgcc ctggtagtct tagtaagctg
    14521 ggtagctaac gactaagagc accacgatga tgagtatctt catcatgacc ctttccttat
    14581 ttaaggcccc ttcctcggga ggggcttgcc cgtttcagcg tcccgctgaa atcgtcggcc
    14641 tacctccttt tgccctgaat gcagtctatc gctaacgcgt agataaaata gtttcctgtg
    14701 ttattactgg atgggtgctc gcaaatgtgc ccgccattca gacgattccc gatagtgttt
    14761 cataattcct ccatttttct ccctgattgg ctggctacac tagtatcatt ccgcgaatcg
    14821 tttcaggaag agaaactctt aacgatgaaa agcagttata aatcccgttg ggtaatggta
    14881 atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg caatgactcc
    14941 cggagtgcag cgccaggggc gacgaaacaa gcgcagcaat cgccagcggg tggtcgccgt
    15001 ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga acaggcagtt
    15061 ccgcgttacc tcaccgggct tggcaccatt accgccgcta atactgttac ggtgcgcagc
    15121 cgcgtggacg gtcaactgat ggcgttgcat ttccaggaag gccagcaggt caaagcaggc
    15181 gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca agcccagggc
    15241 caactggcaa aagataaagc cacgcttgcc aacgcccgcc gtgacctggc gcgttatcaa
    15301 caactggtaa aaaccaatct cgtatctcgt caggagctgg atgcccaaca ggcgctggtc
    15361 agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc gcagctgcaa
    15421 ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa gcaggttgat
    15481 gttggtaacc aaatctccag tggtgatacc accgggattg tggtgatcac ccagacgcat
    15541 cctatcgatt tgctctttac cctgccggaa agcgatatcg ctaccgtagt gcaggcgcaa
    15601 aaagccggaa aaccgctggt tgttgaagcc tgggatcgca ccaattcaaa gaaattaagt
    15661 gaaggcacgc tgttaagtct cgataaccaa atcgatgcca ctaccggtac gattaaagtg
    15721 aaagcacgct tcaataatca ggatgatgcg ctgtttccca atcagtttgt taacgcgcgc
    15781 atgttagtcg acacagagca aaacgctgtg gtgatcccaa cagccgccct gcaaatgggc
    15841 aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa acatctggtg
    15901 acgccgggca ttcaggacag tcagaaagtg gtgatccgcg caggtatttc tgcgggcgac
    15961 cgcgtagtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga agtagtggaa
    16021 gcccagagcg ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc gaaaaaagga
    16081 gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg cgcctgttta
    16141 ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat cttactcgcc gggattatcg
    16201 gttatcgagc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc attcaggtag
    16261 tcacgctcta cccaggtgcg agcccggatg tcatgacctc tgccgttacc gcgccgcttg
    16321 aacgccagtt tgggcagatg tctggcctga aacagatgtc gtcgcaaagt tccggcggtg
    16381 cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc gagcaggaag
    16441 tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct aacccgccgg
    16501 tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgctgtc acctcaaccg
    16561 ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag aaaatctcgc
    16621 agatttccgg cgtcggcctg gtgacacttt ccggcggtca gcgtccggct gttcgcgtca
    16681 aacttaacgc tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg cgcaccgcca
    16741 ttaccggcgc taacgtcaac tcggcaaaag gtagcctcga cggcccttct cgtgcggtta
    16801 cgctttccgc taacgaccag atgcaatccg ccgaagagta tcgccagcta atcatcgcct
    16861 accagaacgg cgcgccgatt cgtctgggcg atgtcgcaac cgtagagcaa ggtgcagaaa
    16921 acagctggct tggcgcatgg gctaacaaag aacaggccat tgtgatgaat gttcagcgcc
    16981 agcccggtgc taacattatc tccaccgccg acagcattcg gcagatgctg ccacagctca
    17041 ccgagagtct gccgaaatcg gtgaaggtga cggtactttc tgaccgtacc accaatattc
    17101 gcgcatccgt ggatgatact cagtttgaat tgatgatggc tatcgcgctg gtagtcatga
    17161 ttatctacct gtttttgcgt aatattccgg cgaccatcat tcccggcgtc gcagtgccgc
    17221 tgtcgttaat cggcaccttc gcggtcatgg tgtttctcga tttttcaatc aataacctga
    17281 cgctgatggc gttaactatc gccaccggat ttgtggtcga tgacgccatc gtggtgatcg
    17341 aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgct ggcggcggcg ctcaaaggcg
    17401 caggtgaaat cggttttacc attatctcgc tgaccttctc gctgattgca gtgctgatcc
    17461 cgctactgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct attactctgg
    17521 cagtcgcgat tttgatctca gccgtggtgt cgctgaccct gacaccgatg atgtgtgcgc
    17581 ggatgctcag ccaggagtcg ctgcgtaaac agaaccgctt ctcccgtgcc tcggaaaaaa
    17641 tgttcgacag gataatcgcc gcctatggtc agggacttgc gaaagtgctg aatcatccgt
    17701 ggctgacctt aagcgtggcg ctcagcacgc tgctgcttag cgtgctgttg tgggtgttca
    17761 ttccgaaagg tttcttcccg gtacaggaca acggcattat tcagggcact ttgcaggcac
    17821 cgcaatccag ctcctttacc aatatggccc agcggcaacg ccaggtcgcg gatgtgattt
    17881 tgcaagatcc ggcggtgcaa agtctgacct catttgttgg cgttgatggc actaacccgt
    17941 cgctgaacag tgcgcgtttg cagatcaacc tcaaaccgtt ggatgaacgt gatgatcggg
    18001 tgcaaaaagt catcgcccgc ctgcaaacgg cggtggataa agtgccgggc gtcgatctct
    18061 tcctgcaacc aacgcaggac ctgaccatcg atactcaggt cagccgcacc cagtaccagt
    18121 ttaccttgca ggccacgtca ctggatgcgc tcagtacctg ggtgccacag ttgatggaaa
    18181 aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac aaagggctgg
    18241 tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc atggcggacg
    18301 tcgataacgc cctttacaac gcgtttggtc agcgactgat ttccactatt tatactcagg
    18361 ccaaccagta tcgcgtggtg ctggagcaca acaccgaaaa tactcctggg cttgcggcgc
    18421 tggatactat tcgcctgacc agcagcgacg gcggcgtggt accgctaagc tcaattgcca
    18481 aaattgagca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc ccggtaacga
    18541 ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag gcgattatgg
    18601 acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcagttccag ggcagcaccc
    18661 tcgccttcca gtcggcgttg ggcagcactg tctggctgat tgtcgcggcg gtggtggcga
    18721 tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc attctctcga
    18781 cgctacccac cgcaggggtc ggcgcactgc tggcgttact gattgccggt agcgagctgg
    18841 atgtgattgc gattatcggc attattttgc tgatcggtat cgtgaagaag aacgccatca
    18901 tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg cgcgatgcaa
    18961 tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg gcggctctgc
    19021 ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt cgtccgttag
    19081 gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttc accacgccgg
    19141 tgatttatct gctattcgac cgcctggcat tgtggaccaa aagccgcttt gctcgtcatg
    19201 aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg gcgacgattt
    19261 tactgtcggt cgccattacc ctgtgcggca tactgggttt ccgtatgctg ccagtcgccc
    19321 cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc ggtgcgtcac
    19381 cagaaacaat ggcgtcttcc gttgccacgc cgctggaacg ttcgcttgga cgcattgccg
    19441 gggtcagtga aatgacctcc agcagttcgc tcggcagcac gcgcattatt ttgcagtttg
    19501 attttgaccg cgatatcaac ggcgcagcgc gcgatgtgca agcggcgatc aacgccgcgc
    19561 aaagtttgct gcccagcggg atgccaagcc gtccgaccta tcgcaaagcg aacccgtcgg
    19621 atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt gaactgtacg
    19681 atttcgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtc ggtgatgtcg
    19741 atgttggcgg cagctcactg cccgccgtac gcatcgggct gaatccgcag gcgctgttta
    19801 atcagggcgt gtcgctggac gatgtgcgca gcgccatcag caatgccaac gtgcgtaagc
    19861 cgcagggggc gctggaagat gatacccatc gctggcagat ccagactaac gatgaactga
    19921 aaaccgccgc cgaatatcag ccgttgatta ttcattacaa caacggcggc gcgcttcgtc
    19981 tgggcgatgt ggcgacggtg accgactcgg tgcaggatgt gcgcaacgcc gggatgacca
    20041 acgccaaacc ggctattttg ctgatgatcc gcaagctgcc ggaagccaat attatccaga
    20101 cggttgacag catccgggca aaattaccgg agttgcagga gaccattccg gcggcgattg
    20161 atctgcaaat tgcccaggat cgctccccca ccattcgcgc ctcgctggag gaagtcgagc
    20221 aaacgctgat tatctcggtg gcgctggtga ttctggtggt ctttttattc ctgcgctcgg
    20281 gtcgcgccac tattattccc gccgttgcgg tgccggtttc gctgattggt acgtttgcgg
    20341 cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc accatcgcta
    20401 ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcccgt cacctggaag
    20461 cggggatgaa accgctacag gccgcgctgc aaggcactcg cgaagtcggt tttacggtgc
    20521 tgtcgatgag tctgtcactg gtggcggtgt ttctgccgct gctgttgatg ggcggattgc
    20581 cgggtcgcct gttacgcgag tttgccgtga cgctttctgt cgccattggt atttcgctgc
    20641 tggtttctct gacgctaacg ccaatgatgt gcggctggat gctaaaagcc agtaagccgc
    20701 gcgagcaaaa gcgactacgt ggttttggtc gcatgttggt agccctgcaa caaggctatg
    20761 gtaagtcgct gaaatgggtg cttaatcata cccgtctggt gggcgtggtg ctgcttggca
    20821 ccattgcttt gaatatctgg ctgtatatct cgatcccgaa aaccttcttc ccggagcagg
    20881 acaccggcgt gttgatgggc gggatacagg cggatcagag tatttcgttt caggcgatgc
    20941 gcggtaagtt gcaggatttt atgaaaatta tccgtgacga tccggcagtg gataatgtca
    21001 ccggatttac cggcggttcg cgggtgaaca gcgggatgat gtttatcacg ctgaagccac
    21061 gcggcgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgtg aagctggcga
    21121 aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt ggcggacgtc
    21181 agtcgaacgc cagctatcag tacacgttgc tgtccgacga cctggcggca ctgcgcgaat
    21241 gggagccgaa aattcgcaaa aaactggcga cattgccgga actggcggac gtgaactccg
    21301 accagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc atggcacggc
    21361 tgggaatcga cgtgcaggcc gccaacagcc tgttaaataa cgccttcggt cagcgacaaa
    21421 tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg gatccgcgct
    21481 atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa ggaaaagcga
    21541 tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc tccgctgtcg gtgaatcatc
    21601 agggattatc ggcagcctcg accatctcgt tcaacctgcc gaccgggaaa tcgctctcgg
    21661 acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg acggtgcgcg
    21721 gtagttttgc cggcacggcg caggtgttcc aggagacgat gaactcgcag gtgatcctga
    21781 ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag agttacgtac
    21841 atccgctgac gattctctcc accctgccct cggcgggcgt tggagcgctg ctggcgctgg
    21901 agctgttcaa tgccccgttc agcctaatcg ccctgatagg gatcatgcta ttaatcggca
    21961 tcgtgaagaa aaacgccatt atgatggtcg attttgcgct tgaagcccaa cggcacggta
    22021 acctcacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc ccgattatga
    22081 tgactaccct ggcggcgctg tttggcgcgc tgccgctggt attgtcgggc ggtgacggtt
    22141 cggagctgcg gcaacccctg gggatcacca ttgtcggcgg tctggtcatg agccagcttc
    22201 tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcga ctgcgttttt
    22261 cgcgtaaacc taaacaagcg gtaaccgagt aaatgacaga tcttcccgac agcacccgct
    22321 ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc accatcgtta
    22381 acaccgcgct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat atgcacatgg
    22441 tcattgtctc ttatgtactg accgtggcgg tgatgctgcc cgccagcggc tggctggcgg
    22501 acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact ctcggttcac
    22561 tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg ttacagggcg
    22621 ttggcggcgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc gtaccgcgcg
    22681 agcaatatat ggcggcgatg acctttgtca cgctgcccgg tcagatcggg ccgctgcttg
    22741 gcccggcgct cggcggtctg ctggtggagt acgcatcgtg gcactggatc tttttgatca
    22801 acattccggt ggggattatt ggcgcgatca ccacactgat gttaatgccg aactacacca
    22861 tgcagacgcg gtgctttgat ctctccggct ttttattgct ggcggttggc atggcggtat
    22921 taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg attgctggcc
    22981 tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga aataacaact
    23041 gcgccctgtt cagtctgaaa ctgttccgta ctcgtacctt ttcgctgggt ctggcgggga
    23101 gttttgccgg acgtatcggt agcggcatgt tgccctttat gacgccggtt ttcctacaaa
    23161 ttggccttgg cttctcaccg tttcatgccg gactgatgat gatcccgatg gtgctcggca
    23221 gcatggggat gaagcgaatt gtggtacagg tagtgaatcg ctttggttat cgtcgggtac
    23281 tggtggcgac cacgctgggt ctgtcgctgg tcaccctgtt gtttatgact actgctctgc
    23341 tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc aactcgaccc
    23401 gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg gcgagcagcg
    23461 gcaacagtct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc actatcgccg
    23521 ggctgttgct ggggcttttt ggttcacagc atgtcagcgt cgacagcggc accacacaaa
    23581 ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg gcgtttgtct
    23641 ttgccagagt gccgagcgat acgcatcaaa atgtagctat ttcgcggcga aaaaggagcg
    23701 cgcaatgaag ttctggcgac ccggtattac cggcaaactg tttctggcga ttttcgccac
    23761 ctgcattgtc ttattgatca cgatgcactg ggcggtacgt atcagttttg agcgcggctt
    23821 tatcgattac atcaagcatg gtaatgaaca acggctgcaa atgctcggcg atgcgcttgg
    23881 tgagcagtac gcccagcacg ggaactggcg cttcctgcgt aataacgatc gctttgtatt
    23941 tcagatccta cgttcactgg agcatgataa caacgaagat aagcccggcc ccggtatgcc
    24001 accacacggc tggcgcacgc aattttgggt ggttgatcaa aataataaag tgctggttgg
    24061 cccgcgagca ccggttccac ccgacggcac acggcggccc attatggtca atggtgcgga
    24121 agttggcgcg gtgatcgcct cccctgttga acgactgacc cgtaatactg atatcaattt
    24181 tgacagacaa cagcggcaaa ccagttggct gattgttgct ttatctacct tattagcggc
    24241 gctggcgaca ttcccactgg cgcgcggttt gctggctccg gtaaaacgac tggtggatgg
    24301 cacgcacaaa ctggcagcgg gtgatttcac tactcgcgta gcccccacca gcgaagatga
    24361 gttgggcaga ctggcagagg atttcaatca actcgccagc acactggaga aaaaccagca
    24421 gatgcgccgc gattttatgg ccgatatttc tcatgaactg cgcacgcctt tagcagtact
    24481 gcgcggcgaa ctggaagcca ttcaggatgg tgtgcgtaaa ttcacgccgg agacgctggc
    24541 ttctttacag gcggaggtcg gtacactgac caaactggtt gacgatcttc atcagttgtc
    24601 gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg gtagatttga tcccactgct
    24661 ggaagtggcg ggcggcgcat ttcgcgaacg atttgccagc cgtggcctga aactgcaatt
    24721 ttccctgcca gacagtatta ccgtatttgg cgatcgcgat cgtttaatgc agttgttcaa
    24781 taacttactg gaaaacagcc tgcgctacac tgacagcggc ggcagcctga aaatctctgc
    24841 cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agcgcgccgg gcgtcagtga
    24901 cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc gaaggttccc gcaaccgtgc
    24961 cagcggcggt tccgggctgg gactcgcgat ttgcctgaac attgttgaag cacataatgg
    25021 ccgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag agttaccgct
    25081 ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa cacaccgcgt
    25141 attttgatcg tggaagatga accgaagctg ggacagttgc tcattgatta tctgcgtgcc
    25201 gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta tgtgcgccag
    25261 acaccaccgg atctgatcct gttagatctg atgctccctg gcaccgatgg cctgacgctg
    25321 tgccgggaaa ttcgtcgttt ttctgacatt ccgatcgtga tggtgacggc aaaaatcgaa
    25381 gagatcgacc gcctgctggg gctggagatt ggcgccgatg actacatctg caagccgtac
    25441 agcccacggg aagtggtagc gcgtgtcaaa accattttgc gccgttgtaa accgcaacgc
    25501 gaacttcagc aacaggatgc cgaaagcccg ttgatgatcg acgaaggtcg ttttcaggct
    25561 tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgtt gaaaacgctc
    25621 tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct ttatgacgac
    25681 taccgcgtag taactgaccg caccatcgac agccacatta aaaacctgcg tcgcaagctg
    25741 gaagctctgg acgaagagca gtcttttatc cgcgccgtct acggtgtcgg ttatcgctgg
    25801 gaagccgacg cttgccgcat cgtttagttt tagcgacatg actttgttag ccggagatgg
    25861 cgttcagatc agatccggca acattatccc acgtatggtc agcaaactga cattatctct
    25921 ccgccctggc aggcaccaga aaatcgctca tactttaatc ggtaaacagc acctttagat
    25981 gctgttttcg atacgcaatt tcaatcaagg agtcattatg gctggttggt ttgaactcag
    26041 taagagcagt gataatcaat ttcggtttgt gctaaaagcg ggcaatggtg agactatcct
    26101 caccagcgag ctgtatacct caaaagcctc tgcggaaaag ggcatcgcgt cggtgcgtag
    26161 caacagcccg caagaagaac gctatgagaa aaaaacggca agcaacggca aattctattt
    26221 caatctgaaa gccgccaatc atcaaattat cggctccagc cagatgtacg ccaccgcgca
    26281 atctcgtgaa accggaattg cctccgttaa agccaatggc acaagccaga cggtgaaaga
    26341 caatacgtaa ttacgatgcc gggcgcgtgt tgttggcgtc cggcgcttta ccagctcccc
    26401 cctacagcgc tacaatgccc gcccttaaag tgggggcact cccctggccg cttcatcagg
    26461 tgaagcggat ctgacctgtc atcagaacga gagaattatg tttaaaccgg aactcctttc
    26521 cccggcggga acgctgaaaa atatgcgtta cgctttcgct tatggcgcag atgctgttta
    26581 tgcgggccag ccgcgttact ccctgcgtgt gcgcaacaac gaattcaacc acgaaaatct
    26641 tcagctcggc atcaatgaag cccacgcgct ggggaaaaag ttttatgtcg tggtcaacat
    26701 tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac ctgaaaccgg tggtggaaat
    26761 ggggccggat gcgctgatta tgtccgatcc agggctgatt atgctggtgc gtgagcactt
    26821 ccctgaaatg ccgatccatc tctcggtaca ggctaacgcc gtgaactggg cgacggtgaa
    26881 attctggcag caaatgggcc tgacccgcgt gatcctctct cgcgagctgt cgctggaaga
    26941 gattgaagag atccgcaatc aggtgccgga tatggagatc gagatcttcg ttcacggtgc
    27001 gctgtgcatg gcctactccg gtcgctgcct gctctctggc tatatcaaca agcgcgaccc
    27061 gaaccagggt acctgcacca acgcctgccg ctgggagtac aacgtccagg aagggaaaga
    27121 agatgacgtt ggcaacatcg tacacaagta cgagccaatt ccggtgcaaa atgttgagcc
    27181 gacgctgggt atcggcgcgc caaccgacaa agtgtttatg atcgaagaag cccagcgtcc
    27241 gggcgagtat atgaccacgt ttgaagatga gcacggcact tacatcatga actcgaaaga
    27301 tctgcgcgcc atcgcccatg ttgaacgcct gaccaaaatg ggcgtgcatt cgctgaaaat
    27361 cgaaggccgt accaaatctt tctactattg cgcacgcacc gcgcaggttt accgtaaagc
    27421 tatcgatgac gccgctgcgg gcaaaccgtt cgataccagc ctgctggaaa cgctggaagg
    27481 tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt catacccacg acgattatca
    27541 gaactacgaa tacggttatt cggtttctga ccgccagcag tttgttggtg agtttaccgg
    27601 tgagcgcaag ggggagctcg cggcggtagc ggtgaaaaat aaattctccg ttggcgacag
    27661 ccttgagcta atgacgccgc aaggcaacat taactttacc cttgagcata tggaaaacgc
    27721 caaaggtgaa gcaatgccgg tcgcaccagg cgatggttat actgtgtggc tcccggtgcc
    27781 gcaggatctc gaactgaatt acgcgctgct gatgcgtaat ttctccggtg aaaccacgcg
    27841 taacccccac ggtaagtgat taatttcgat tatttttccc ggatgaaaaa ttcttagaaa
    27901 ccgatcacat acagctgcat ttattaaggt tatcatccgt ttcgctgaaa aacataaccc
    27961 ataaaatgct agctgtacca ggaaccacct ccttagcctg tgtaatctcc cttacacggg
    28021 cttatttttt acgcgtaata caatgaaata aaaggattta tttctggtca cgtccacaca
    28081 ttgaccacat cgacaaaaaa gcccctcgac tgaggggctt cctgtttgta attacatcca
    28141 cataatttgc tgccctgacg gcaacgggtg cggtcttacg gcgtggactt ctcccggctt
    28201 cacgatgtat cgctgtaccg actcataagt gatgaacgtg gcgctgcaat tcacgttctg
    28261 gcactggtga taacgctctt ttgtcgtgtc agtgatatag cgacttgtac gcgcatgtgc
    28321 ggcatgctgg cataaaggac aatgaaacat cgcgagcacc tcttccggtt ttgttgatgg
    28381 tgccatttta gttaatttat ccttataaaa caaacagata aaacaaaaac atcactcatc
    28441 atcttctgtt tcgtactcca catcagaaag cctgacctca agctccaagg acgtcgtgaa
    28501 gccgctatta ttcagaaaat gtgtcacctt agtgattgtc cagtcctgct cgtctatgac
    28561 gcgcttaaag cctgacactt tgaccggcgt ttccgtgtaa atatctgccc gaccagtagc
    28621 cagactgatg gagaactccg caacgccccg ttgcagctta tcccacttcg cctgagcggc
    28681 gcgcatggcc tgtgctttcg tggcatatac cgtggtcagg gcaaaaacgt tgtcagcctc
    28741 accagccatg tattcacctt cgcgcgcttc cggtactttt ggcgctttct tctgcgtgac
    28801 tggtttcgct ttcgggtgct ccagtgcgcg caggtgtttc tctttctttt tgcgtttcag
    28861 ttttaccttc tgcttttgtg gcttcgggtc tttggtgtgt aaccactttg ccgttacgcc
    28921 ggtataggct ccacggtcag caatcgcaaa atgatggcgg tcgccgtcgc tgcgggttat
    28981 ggtgacctgc gggatttttt taccgctggc cgtcaccccc tgccccgctt tgagaaacaa
    29041 cagttttccc atttttaccg acacctcacc gccgttgcgt tctgcaagac gggtcaggaa
    29101 tttcgcatca gactcctgcg actggtcgat gtgcgggatt ttaattccgg ccagtgacgg
    29161 agcgacactg gcttccagcc tgttacggga ggctatcgcc tcaacaatcg caccgagcgt
    29221 ggtgtcatgc caggagcctt cacggcggga attgagcgtc ccgcgaaaat ctgcactccg
    29281 ggcgcggatg gtgaccacat ccggtgcgcc ccggtgttca acctcatcaa cggtaaattt
    29341 ccctttgcat accagggcaa aacctttcca gccgatatac accgtcagga cagcgccacg
    29401 aaccggcagc ccgacctgcc cgtcggcatc gttcagttca atatcaagct ggtcagcctc
    29461 aaagccccgg ttatccgtca gggtcatgct catcagtcgg tcgctgatat tgccggaaat
    29521 atccctgctg tcgagcatca gcatgtaatc cggcgtcagc gtactgcctg catcaaatgt
    29581 cagtgcatcc agcattatcc cgcccccgtc atacccgtga atctggtcgc catactgcca
    29641 gccttaccga tgagcgattc cgcctgttta ccgatatcgc cataaagcgc ggccagtgat
    29701 tcatcaacgc gggtgagcga cagcgtaaaa tcaatttttc ggggtgtgcc gtctgcaaag
    29761 aaaatacttc ctgtttcact caccctgctg atgacataca tgccgtaaat catgccggtg
    29821 ccatccagca acggccacgc ccggccttcc tctgccatca gcctgagcgt ggtcatcgtc
    29881 agcttgccgc cggtcagttc tggataaagc acaccggcaa gcgtgatgtt ttcctcaccc
    29941 acaccgagaa actggtaggc gtcccgttta ccgatacggg aatttgacgg ccagcgataa
    30001 tctgattcac gctgcatggt ctggtgtggc agcgtctggc gcataaaaac aaacatacct
    30061 aacgcgagca tcatttttcg tcacctcctt aaccgtcatg catcatgctg gcacgggcgc
    30121 gcgcacgttt atcccgctcg tatttttcga gcgcatcctg taactggcgg tcaagctgtg
    30181 tccccggcgc agtaccaccc gtcaggctga tgtgatattc gtttttactc tggtccacat
    30241 aagagcggcc agccggtgcc gtgaccggct gataagcctg atagcctgca taagagctgg
    30301 tcgccggaat ataaccaccg gtgccatacg tggcggcatg agttctggcg gcggtctggt
    30361 caagtgtgtc tgactctttg ttgataacac cgagtttttc cagtacccag tcaataccgc
    30421 tgcgcagttt gttgaacgca ttaagcggca gcatcagcgc gtcagccagt gcctgcccga
    30481 acatgacgcc cgtgtcacgg caacggttca gggtgtcctg tgtggcttta accggagcaa
    30541 tcaggtttgt aaaccactgc cacgcggcct gtaacttttc gcccagccag tcaaacaccg
    30601 gcttaagtgg cgtgaacagc tcccccaccg gcgcaaatgc cgctttcagc ccttcaacca
    30661 caccgccaaa gaatgcgctg acaggctccc agtatttacg gataagcaac gccccggcga
    30721 caatggcagc caccacggcc acaaccggcc agctaatcgc accgatggcc gtcataacgg
    30781 cactaccaac cgtcgtgaag attgccccca ttgcgcctgc tgccgcgata atggcattga
    30841 tgccggtgat aaccggccag gctacaaggc caatggcacc gatgatgcca gtcagcacca
    30901 gtgcaccgcc ggcaatgatg ccgatggttg acgccagtga tttgtttttc tgtatccagc
    30961 cgtcgagttt taacacatac tttgtggccg tctgcgtgag cttacgcagt gcgccttcct
    31021 gctggtcaaa caggtcagtc cccaccgcct cataagcgga ctgaaactcc ttaaagtcac
    31081 cgccgagatt gtcctgcata atatttacca gctctgaggt cttcccgtcc gaggctttaa
    31141 acgcagcggt cagtttgtcc agctttccgg ttgaggcgac agtcatcagc acggcggcgg
    31201 ctgagctggc ctcctccccg aaaatggttt tcatgtattc agcctgctgg gcagtaccga
    31261 gccggttttt ctcaaaactg gcctgcattt ctttcagaat ggtaaatact ggtcgggtgt
    31321 ttcccttgct gtctgaggtt ttcactccaa gctctttgag tgcatcccat gcttttcccg
    31381 tcggtgcctg caggcggctt aacacggcac ggcttcccgt ccccgccatt gagcctgtga
    31441 tttttgcatc atgcagcgcc ccgaccattg cggcggtttc ttcaatgctg acaccggcat
    31501 tttttgccac aggtgcggca taggtcagcg catcgctcat gccgtcaaaa tcggcggcgg
    31561 ttttgttcat cgtcatggag agaacatccc cgatatgagc gaccttatcg tttgaaagct
    31621 gaaaggcgga tttcatcccc atcagcaggg cggcgttttc ttccatcgtg cggcggttcg
    31681 ccagcgccat attcagcgtg accggcgttg ccgcctgaat ggcatcaaca tccccacccg
    31741 ctttcgcgat gattatctgt gcaccggccg catcatccgc cgaggcggcg gtattgtcgc
    31801 cgagctggcg cgcctgtttg cgtagtgcgg tcatttcggc ggagtctttt gccacaccca
    31861 gcacggcctg caattctgag tttttctgcg caaactcata accggacatc agcaacttaa
    31921 ctccggccat cgtttccgcc gccgcaatcc ccacaccggc agcgcccact gaggccatat
    31981 ttccggccag ttcctttccg gcctgataac gctgttttac tgcattaagt tttgcctgtt
    32041 gcgcactgac acgcgccagc gcgtcacgct gccggttaag ctgtgcggtg gtttcactga
    32101 tacggttttt cagtccctgc tcatcatgtg caagattgcg ggtattaatt cccacagcgg
    32161 ccagttcccg ctgctggcgc ttaacggaat ccgtcaggcg gttatatttc gcctgtaagt
    32221 cctccgccgc acgctttgcg gattccagca ctttcgcctg agcacgggtc ggacgttcag
    32281 tgtttttaaa ctgtgtggca agtgcttcgg cttcctgccg tgccttttca agtgcatgac
    32341 cagtcacggc aagctgtgcg ctggtcttgc gaaatccctc aatacgggat gcgtgaccgt
    32401 tcagctcgcg cagtgatttt tgtgtttccc ggatatcccc cgacagcgac ttactcgctg
    32461 tacggatgga tttaaacggg cgggatgcct ggtcaacagc cctgagcaat acctgtaatt
    32521 ttacattgtt actcattcgt gcttccgctt cgccggagcg ccttttcgcg ccatgtgatg
    32581 agttcggtca ggctcatggg atacagttct gatggcggcc agtgaaatat cactgccaca
    32641 tctgccatca ggtcatcgac cgacagattt ttcggaaacg tcactgcacc gagttcggag
    32701 acaaaaaacc gaccacctta ccggccagcg ccacaaggtc aggcagttcc agcgcggcga
    32761 cctcctgctc ggtcagcatc ggtgccgtca tgcgcggcag caccttaatc agtgcatcga
    32821 cttcggagtt tgcgaccgca gccagactga caccgcgcag cgtcccggca ttaggtttca
    32881 tcagcgtgac ctgttcgata acctgctcac cacgcttgac cggattgtcc agggtaatca
    32941 cattttcttt gttcatggtt ttctcacttc tgaatcgggg ttaaccggtc agccaggctg
    33001 accggatgaa aatcacaggc cgatattgcg gcggtgttgc tccagccggt cgacgccgtt
    33061 caccttctca atcatgttga tggtgtcgat ttcgaccagc tccttaccgt ccatcgtcaa
    33121 cttgaaataa gtacaaatca ccgggagttt cgattcggta tcctctccct gtttaccctc
    33181 tccggtatcg acttctttct gacgcccacg catgaccact tcgacggcca ccgtttcgcc
    33241 ggtatcgtcg cgctggtaag aaccagcaaa acgaatcggt acggcatcag cactggttgc
    33301 agcgtaaagc tcccagataa ccgaatccgg gaatccaccg agcgaccact ccattgacag
    33361 agcatcatca tcaaggccga ggtctaccgg tgcgctgccg ttcatccccg caccgcgata
    33421 gttttcgagc ttacgggtca gttttggcag cgtgacggac tttgcaacgc cctgatagct
    33481 gtagccgttc agaaagacgt tcattaactt gagtttgcgc ggcattgcca tcggtcaggc
    33541 tccttaattg ctgttaaccg aagtgaccag acttgccagg tatctggaag taatacgctg
    33601 gcgcagggtc aggttttcga gaggaggcac cggggtatag tcgtagtcga tatacagttt
    33661 tccggccttg agggtttccg catcgttgga ttcttcgctg aaccagcatg tgccatccac
    33721 gatataaccg ttgtttttca gttcacggaa tttggcattg atgccgtcaa tgatgtcgcg
    33781 aatcagcgtt gcggtgatgg gcttatcaat agcccacatg tgcgcctcag ccatcgtgtc
    33841 ggccagtacc tgcgcggtgc gtgtgtagct ttcaaagagg aacagcgggt catcagagca
    33901 ggtacggtta ccccagaaac ggaaaccgtc gcggcgaacc agcgttgtga cgcctgcctc
    33961 gttcagcagg tcagcatcag tgcctggctt ctgcaaatcc cagaagacgg atgcactgat
    34021 gccggtaaca ccgttcaccc cgacgttgga cagcgtttta tgccagccct gctcctggtc
    34081 gattttagcg cgcagaccca gcgcacgagc ggtggcatac gcggtggcgg tggtactggt
    34141 aaccgtatcc catgcgagga aatccggcca gataaccatc agctcacgct ggctgaaatt
    34201 ctggcggtag gctttcacct cggaaatggt tttacaaccc catgcgctga tatacccgaa
    34261 ggcattcagt tcctggcata ccgatgccag tgcgacggcg acctctttgg tgtccagtcc
    34321 cggtacgccg agaatgcgcg gtttaacgcc ggtaaccgac tcagccccca tcagggcttt
    34381 cagtccggtg tactgaccgt tttcgtcggt ggtgccgata atattggaaa cagtctgcgc
    34441 gagtttcgtt tcctcgtcgt cgccggtgcc gtcttccacg cgcacaacaa cggtgaccgg
    34501 ttttgactgg tcagcgatgg cctgtaacga cgccgccagc gtgccttttt taccggcctt
    34561 tgcaattgcg ctctgcacat tggtaatcag cacaggttta ttgaggggga atgtttccgc
    34621 atccgcatcg ctggccgtgc agaccatgcc gacaatggca gtggatacgg tggaaatgac
    34681 gcgggtgccg tcgttaatct ccagcacctg cacgccgtga tgatagtcac tcatccgtct
    34741 aactccgtgg ttaatgggtg agtggtattt tctgttgtgc agagcatgag acgctatttg
    34801 acctggctgg tcagtggatg aaacaacaga taaagaaaag gcggacaatc cgcccgcctg
    34861 tcatgatttg tactcactta tttcccgact gacaatttac atagccaaaa cgctatcaaa
    34921 tctgacagtc tgctttgagc gagaagcgga agttcaatgt gataaagctc aggaacattg
    34981 tcgttgatga tgataaagag gatcagacta acttagcact tctcatcacg ttgataaggc
    35041 atagaaactg caaacactat tgtgaatata atttcactat agtaaattaa agtaagctct
    35101 tttcgttcgt tattacataa aataaagttc tctctaaggt tgaaaaatgt tagatatcag
    35161 aatgtcacga ccatctgaag catcagagat catacaaatc tggaagagtt cagtggatgc
    35221 tacccacgac tttcttacag gtcatgaccg acaagaaatc gaaaaagaag ttatcggctt
    35281 tttctcagaa actcctgtat gggttgcaac aaatcaggat gaccaaccgc taggatttat
    35341 gtttttgcat gaggggcatc tggaggcact ttttgtagca ggctctgctc gcggacttgg
    35401 cgtcggaaaa cgtttgatat ctcatgcact ggcgctgcat ccagagctaa gcgttgatgt
    35461 aaatgaacaa aaccagcagg ctgtagggtt ttatcagcat atgggttttc aggtatcagg
    35521 gcgttccgat ctggataatc agggaaggcc ataccccttg ttgcatctga gaatggccaa
    35581 taacatgtaa ccaatgtggc agagaagata tcattgattt ttttggctaa aagccacgat
    35641 gtaagaacag ctctgctcac tatctggatt gctcatttgg tactcgcttg caaaggtaag
    35701 ggcaagacag tcaccggcac gtaaatgatg ggtctgattt ttgactcgaa aatcaaggct
    35761 gccactcatc atccaaagcg tttgcccgga aagatgctca tttgcggagg cgggtatcgt
    35821 cagctcaccc attgcaggga tttcaacttt gatcagttcc gggcatgccc ccgcaggaga
    35881 caaagaccaa cgggtgatgc ctgattgctc atccgtccag tgctgttgct gatgagcaaa
    35941 acgaaccagg gaattctgat gttgctcaag ctctgcaaag agtttagata gcgttacatt
    36001 catggcgttg gccaggcggc taaggatagt tgcactaggg ctggagatgc ctcgttcaat
    36061 cttactaatc atggcctgac tgacgcccga tcgttgagca agctctgtta cagtcaaatt
    36121 gcgagccttc ctgtgtttaa gcaataaacg tgcaatatcg ctatctacta tctgattttt
    36181 aaatttttta tccatttccg ccctctcttt caccaacctg attatgccat tacataaatt
    36241 ggcctgaaat aaatgatttt gtaagtctgt aaattcacta ctcctaagcg cggcacttcg
    36301 tttatacgca cctataacct taaaatatga gttggtaagc ttaacgtccg cttttggcac
    36361 atagcggact gtcagattag gctttactct gtgctataga tatgtaagct cacaccagag
    36421 atcatacaac ttattgcggc atttccggcc attcgggatt tgcaggatcc acacgactga
    36481 ccagaacgct gtagcgttcc catgcttcca gtcggctgcg ttcctcatcc gtcgccatat
    36541 tcagcctgac agcgcgttcc agcggcaaaa tcacggattc agcttcggaa agtaaagctg
    36601 ccttttgtga ttctgcctgt tgctcctgtt catctgccgt ataaatccgc ttaatcacgg
    36661 caccatcctt aaacatccat ttacctgagt catcagcacg tcggttggag gtaatatcag
    36721 gaacctcgac aacgctgaaa ccttcagggt taagcgttga agcatctctg gtgatgccga
    36781 caattatatt attctcgtcg taaacaatct ttatcgtgtc ttcctgaaaa ttacttactt
    36841 cctcatacca gttttttccc tcttcggacc ataaccagat aacatcaaaa ttttttgtca
    36901 gttgatattg ggcaacagtt tttggattac ccgcagtaat attttttaaa tgctgcataa
    36961 attacacctg tgcgacgtta taccatgtgc cattgatgta tttttgtatt ggcctgaata
    37021 ctgcggggtc atcaccatcg acttcaccga caataccaag cccggtaatt acgtgccctg
    37081 ctttctcata catcacccct ttctgcatgg tctggacaac acgtgtgcca agtctgatat
    37141 ctctcacata gcgggaatca aagttaccgt aatccgaggg attaacacgc cccgtaatat
    37201 ttatggtctt attactttga atgcttccgg aaacaaagcg cataacatgg acgttattag
    37261 cataaacatc cagattacca tcgccatttt gtttaaatcc ggtgtcgtta tcaccaagaa
    37321 caatagagtt cccacccaac gcgttattcg tgccaagtgc cagcccgcca tcaatcctgg
    37381 caccattacc aacagacaca actcctgttc ttaagttgat gccgaatggc cttaatgacc
    37441 caatatctcc attttcaccc tcattttctc gtgtaggaat gatatacagg ttttcttcag
    37501 aacggcgaaa aatagcacca aaagatgaat taaatatcct cagcgcattg actgtagata
    37561 tttttacttc actactgaaa agggctttaa caagaacata cagagcatcc catttaagat
    37621 tcatcaggtc ttttgttgtg gtgctctggc gacttctcca tttgaaatat tcattgccgt
    37681 tgtcacctgt ttcaaaccac atatatgaat cagtatcgct gtcggcatca tttttaaatc
    37741 caatctttgc ccagtcagta tttcgaatcc aggcaaggat tgagtcgttt tcaaaagtaa
    37801 gcccgccgga caaggtatcg ccattttttt gcacggcgtt cccggctcgg tttaccgttt
    37861 cctgtaaacc gagatattcg ataacggcgg caacggtcga tttagccaga atatcccgcc
    37921 cgacttttgt cagggtcgcc aggctggcaa catcattccc cgtaaaatac ggaaacctgt
    37981 ctgccgcagt agcaagcccc gccagcgccg tcaaagtggc atctttcggt tgcttacccg
    38041 caagcgcatt agtcatggtg gtagcaaaat tcgggtcatt gcccagcgcc gccgccagct
    38101 cgttcagcgt attcagtgcg tcaggcgacg agtctacaag ggcggcaatc gcggccataa
    38161 cgaaagccgt gtttgcgatc tgagtattat tcgttccctg tcgcgcagtt ggcgtcgttg
    38221 gcgttccggt cagtgcaggg ctgtttaatg gcgctttctt gttcgtttca tccattaccg
    38281 tcttaacggc ttttggtgtt gcggcgagcg tttcagacat gctgttggtc gcactactta
    38341 gctggacaag accttttcgc gctgtggtag cgtcctgtgc ggtatatttc ccgttagcaa
    38401 ggtcatacgc ggcctttacc gctttcggcg ttgcggccag tgtttcagac gtgctgttgg
    38461 tcgcactgct taactgagta aaacctttgg cggtcagcga ggcgtccggg tgacgtcgtg
    38521 actgttcgtg ctctgcaatt ttgtcatcaa cgtaatcctg tgtcgccatc accgttgtgg
    38581 tgtcaatggt cagctccacc gaggccacac tgctgacgat gatgaccatg cgacaggtct
    38641 gcgaacgccc tgagccttcg gcaagagctg gcttataact ttcggccatg ttcgccacgg
    38701 caattaacgt tcccgcatca tcgtacaggc caagctcacg catccagaaa ccgcccacct
    38761 ccggcggaat aaccagctct gcgataatat aattactgtt tcgtttgtcc tggctgattt
    38821 tgttcagcgt atgtcgccag acttcgtgga taagcccggt ctgtccggca tccgggacag
    38881 gcaatttacc accgccatcc ccgacggcca tcgtggtaat gttgaccttc cgccctcccg
    38941 gtgcggttgc cgctgccagc tttgctgcac cggcagtggt gataacggtt ttgaattttg
    39001 tgctcattat tcctcactta tccggggtaa accgtaatta catcgccgtc gtaagccaca
    39061 ccaccggcga acaggtagcc gggaatgtcc cgggtaatgt tcaggccaat aaggtggcgg
    39121 cttgcaggtt tggcatcagc aatcagccgt tccatttcct gatacattgc ctctgtgatg
    39181 ccactttcca gtacaccaat atcaagccgg aaggtgcccg gcgggtcact ggtttcccac
    39241 cactccgtca cgttgatgag atagcccagc ggctccacca cacgccggat tgcacctata
    39301 gtgcctttat gacagtggat gaaataggca tcgcggataa cagcgcgttt tgtcgcttcc
    39361 ggccactttt catcccatct gtcgaccgaa aacgcccacg ccagccacgg cagcagattt
    39421 gccggacagg agtccgggtt ccacagctca cgaatactga ccggtgtttt ttcaatttcc
    39481 gcacaggctt ttgcggcggc gacctcaagc ggtgatgagc cggtcggcaa cagtcgcgaa
    39541 tcactcatcc gagcctccgg tcacgacgcg gtattcggta cagaaagacg cctgcgtact
    39601 gttgagcacg atgtcggcca gtggtgcggc cagctcgaca cgctgcacgc cttccacatg
    39661 caaagcggca taaatggcag acagacggat gtcgcgcccc agccggtgct gtgccgtgat
    39721 gtacgcttcc agttttttca cggcggcagc gcgtatgggt tcgctttcgg gaccagggta
    39781 aaggtaaagc gtggcgttta tctggtattc aacgatggcg gcagactgca cggtcacgcg
    39841 gtcggccacc ggcctgacgt cctcgccatt aagggcgtta cgcaccaccg ccagcaggtc
    39901 ttcggatgcg acaccgttat tttcacgtga cagcacagag atggtgacgc aggccggaga
    39961 cggactggtg acagagatat ccgcgacacg cccgtcagca ctgcgaccat gatactgata
    40021 ggcacccacc gacccggcga cgcttaaacc ttcaaaggcc tgctgaatac gcagacgata
    40081 atcggtgtca gattccatca cggccggtgt cggcgggatg gtcgaatcat ctgccggggt
    40141 gatagtcaga cgcgtggtgt tgtaattggc accaatcaca tcaaggtcat tacccgcggc
    40201 acaggccagc atcaccgccc gtgcggcctc attcacacgc tgacgccaga gaagctcacg
    40261 ataagcattt tcctccagca gtttgacgag aggctcagat tccagcgtca gggtacgggc
    40321 gaccgcctcc tgctggtctt ccgggtaaag ggaaatcagt gtcgccttgc gttcggcgag
    40381 aatggtttca aagtccagct cctcgaccac atccggtgcg ggtagctggt tcaggtcgat
    40441 aatcggcatg gtttcaactc acagggatgg ttaacgaaag tggctggccg gtgtcgttgt
    40501 gctggccggt taacgtgacc gtcattcgcc cgtcaaaact gcgcgccgtg gtgacagatg
    40561 acagcgtgac gcggggttcc catttcagca ccgccatgta acaggcgacc ttaatctgca
    40621 actcaagcgc cggggtctgc ggctggtcaa tcattgatgc cagcaacgag ccgtaatcac
    40681 gacgcatcac ccgtgagccg accggtgtac gcaggatatc gccgatactc tggctgatat
    40741 gctcaaggtc agtgacagtc aggccattac tgcgattcat tccgagataa cgagctgtca
    40801 taaaggactc ccggttgtgc cgccgctgtc gccggggtgt ttgtgggtat gcagtacctt
    40861 accgtttgat gagagttcac cgccggtgtg ttcaatgttg ccacgcatcg tcccgccctt
    40921 ctgtacttcc agcgtgctag taatcagcct gttggtgcag accacctccg gtgtgtccag
    40981 ggtgatgcga gttgacgctt tcaccatgac caccggcacc gtggcagtaa cagaatcaga
    41041 agccgtcacg ctggccgttt taattccgct taccgtgagt gcactggttt cgggttcata
    41101 ctcaatcacc gccccgtcag ggaaacggat atgcagggca tccgccgacg cagacggcgc
    41161 ggggttatcg ccggaataaa tccccggcag aacgaacgcc gtgtcaagtt caccacccac
    41221 ggccagaatc agcacctgtt cccccacgga aggtgcccac catgtgcgcg aacgcccggc
    41281 gcgatgggtc agccactgaa gccagtcggt gcacatgccg ccggtctgca cacggcagcg
    41341 accggcgtta aggtcggttt cgacgacaag gccggtacga atcatgttgc gcagtgcgcg
    41401 cgcgagttcc tgaatatttg cgagagtgtt catgcgtgtg agattgcaca atatataaaa
    41461 gttatgctat ctggattcat ttgtagaacg accatacaac attcgaggag agcgtaatgt
    41521 tcagtgataa t
//
LOCUS       DAZUCC010000016        40490 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZUCC010000016 DAZUCC010000000
VERSION     DAZUCC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.016"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1753
                     /gene="adeD"
                     /locus_tag="ACHY09_000753"
     CDS             <1..1753
                     /gene="adeD"
                     /locus_tag="ACHY09_000753"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS3883766.1"
                     /translation="NHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEIS
                     GPIVIKGRYIACVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETAT
                     LPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGAS
                     FTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKEL
                     NTYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCML
                     CTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGK
                     QADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTISRQPVSASD
                     FALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACG
                     LLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVIRNGQVQSH
                     LPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQ
                     GLFDGEKFAFTTLEVTE"
     gene            complement(1800..3191)
                     /gene="uhpT"
                     /locus_tag="ACHY09_000754"
     CDS             complement(1800..3191)
                     /gene="uhpT"
                     /locus_tag="ACHY09_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFS3883767.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQSFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3447..4766)
                     /gene="uhpC"
                     /locus_tag="ACHY09_000755"
     CDS             complement(3447..4766)
                     /gene="uhpC"
                     /locus_tag="ACHY09_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFS3883768.1"
                     /translation="MLPFLKAPADAPLLTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGVSALLLLPFLNAQTPREA"
     gene            complement(4776..6278)
                     /gene="uhpB"
                     /locus_tag="ACHY09_000756"
     CDS             complement(4776..6278)
                     /gene="uhpB"
                     /locus_tag="ACHY09_000756"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFS3883769.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6278..6868)
                     /gene="uhpA"
                     /locus_tag="ACHY09_000757"
     CDS             complement(6278..6868)
                     /gene="uhpA"
                     /locus_tag="ACHY09_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFS3883770.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6941..7231)
                     /gene="ilvN"
                     /locus_tag="ACHY09_000758"
     CDS             complement(6941..7231)
                     /gene="ilvN"
                     /locus_tag="ACHY09_000758"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFS3883771.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7235..8923)
                     /gene="ilvB"
                     /locus_tag="ACHY09_000759"
     CDS             complement(7235..8923)
                     /gene="ilvB"
                     /locus_tag="ACHY09_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFS3883772.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAIAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLTHYGLINAVAACVDDSAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9029..9127)
                     /gene="ivbL"
                     /locus_tag="ACHY09_000760"
     CDS             complement(9029..9127)
                     /gene="ivbL"
                     /locus_tag="ACHY09_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFS3883773.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9691..9780
                     /gene="tisB"
                     /locus_tag="ACHY09_000761"
     CDS             9691..9780
                     /gene="tisB"
                     /locus_tag="ACHY09_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFS3883774.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9904..9978)
                     /gene="ysdE"
                     /locus_tag="ACHY09_000762"
     CDS             complement(9904..9978)
                     /gene="ysdE"
                     /locus_tag="ACHY09_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFS3883775.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10060..11244
                     /gene="emrD"
                     /locus_tag="ACHY09_000763"
     CDS             10060..11244
                     /gene="emrD"
                     /locus_tag="ACHY09_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFS3883776.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11252..11749)
                     /gene="yidF"
                     /locus_tag="ACHY09_000764"
     CDS             complement(11252..11749)
                     /gene="yidF"
                     /locus_tag="ACHY09_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS3883777.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11746..12108)
                     /gene="yidG"
                     /locus_tag="ACHY09_000765"
     CDS             complement(11746..12108)
                     /gene="yidG"
                     /locus_tag="ACHY09_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFS3883778.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12098..12445)
                     /gene="yidH"
                     /locus_tag="ACHY09_000766"
     CDS             complement(12098..12445)
                     /gene="yidH"
                     /locus_tag="ACHY09_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFS3883779.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(12505..13998)
                     /gene="yidJ"
                     /locus_tag="ACHY09_000767"
     CDS             complement(12505..13998)
                     /gene="yidJ"
                     /locus_tag="ACHY09_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS3883780.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGDNILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(13995..15710)
                     /gene="yidK"
                     /locus_tag="ACHY09_000768"
     CDS             complement(13995..15710)
                     /gene="yidK"
                     /locus_tag="ACHY09_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFS3883781.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     MGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            15877..16743
                     /gene="yidL"
                     /locus_tag="ACHY09_000769"
     CDS             15877..16743
                     /gene="yidL"
                     /locus_tag="ACHY09_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS3883782.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(16833..18494)
                     /gene="yidE"
                     /locus_tag="ACHY09_000770"
     CDS             complement(16833..18494)
                     /gene="yidE"
                     /locus_tag="ACHY09_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFS3883783.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(18690..19118)
                     /gene="ibpB"
                     /locus_tag="ACHY09_000771"
     CDS             complement(18690..19118)
                     /gene="ibpB"
                     /locus_tag="ACHY09_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFS3883784.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(19230..19643)
                     /gene="ibpA"
                     /locus_tag="ACHY09_000772"
     CDS             complement(19230..19643)
                     /gene="ibpA"
                     /locus_tag="ACHY09_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFS3883785.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(19637..19709)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            19949..20281
                     /gene="yidQ"
                     /locus_tag="ACHY09_000773"
     CDS             19949..20281
                     /gene="yidQ"
                     /locus_tag="ACHY09_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFS3883786.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(20283..21497)
                     /gene="yidR"
                     /locus_tag="ACHY09_000774"
     CDS             complement(20283..21497)
                     /gene="yidR"
                     /locus_tag="ACHY09_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFS3883787.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLDSTLDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSNEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            21598..22662
                     /gene="cbrA"
                     /locus_tag="ACHY09_000775"
     CDS             21598..22662
                     /gene="cbrA"
                     /locus_tag="ACHY09_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFS3883788.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTMLKEKMNAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(22659..23996)
                     /gene="dgoT"
                     /locus_tag="ACHY09_000776"
     CDS             complement(22659..23996)
                     /gene="dgoT"
                     /locus_tag="ACHY09_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3883789.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(24071..25219)
                     /gene="dgoD"
                     /locus_tag="ACHY09_000777"
     CDS             complement(24071..25219)
                     /gene="dgoD"
                     /locus_tag="ACHY09_000777"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFS3883790.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(25216..25833)
                     /gene="dgoA"
                     /locus_tag="ACHY09_000778"
     CDS             complement(25216..25833)
                     /gene="dgoA"
                     /locus_tag="ACHY09_000778"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFS3883791.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEKSIPAIVDAYGDKALIGAGTVLKPEQVDALARMSCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(25817..26695)
                     /gene="dgoK"
                     /locus_tag="ACHY09_000779"
     CDS             complement(25817..26695)
                     /gene="dgoK"
                     /locus_tag="ACHY09_000779"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFS3883792.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGFNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(26692..27381)
                     /gene="dgoR"
                     /locus_tag="ACHY09_000780"
     CDS             complement(26692..27381)
                     /gene="dgoR"
                     /locus_tag="ACHY09_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFS3883793.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            27658..28314
                     /locus_tag="ACHY09_000781"
     CDS             27658..28314
                     /locus_tag="ACHY09_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883794.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(28358..29170)
                     /gene="yidA"
                     /locus_tag="ACHY09_000782"
     CDS             complement(28358..29170)
                     /gene="yidA"
                     /locus_tag="ACHY09_000782"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS3883795.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTTLSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(29285..29683)
                     /gene="yidB"
                     /locus_tag="ACHY09_000783"
     CDS             complement(29285..29683)
                     /gene="yidB"
                     /locus_tag="ACHY09_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFS3883796.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            29801..29902
                     /locus_tag="ACHY09_000784"
                     /pseudo
     CDS             29801..29902
                     /locus_tag="ACHY09_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29923..32337)
                     /gene="gyrB"
                     /locus_tag="ACHY09_000785"
     CDS             complement(29923..32337)
                     /gene="gyrB"
                     /locus_tag="ACHY09_000785"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS3883797.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(32366..33439)
                     /gene="recF"
                     /locus_tag="ACHY09_000786"
     CDS             complement(32366..33439)
                     /gene="recF"
                     /locus_tag="ACHY09_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS3883798.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(33439..34539)
                     /gene="dnaN"
                     /locus_tag="ACHY09_000787"
     CDS             complement(33439..34539)
                     /gene="dnaN"
                     /locus_tag="ACHY09_000787"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS3883799.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(34544..35947)
                     /gene="dnaA"
                     /locus_tag="ACHY09_000788"
     CDS             complement(34544..35947)
                     /gene="dnaA"
                     /locus_tag="ACHY09_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS3883800.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            36244..36324
                     /locus_tag="ACHY09_000789"
     CDS             36244..36324
                     /locus_tag="ACHY09_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883801.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            36554..36694
                     /gene="rpmH"
                     /locus_tag="ACHY09_000790"
     CDS             36554..36694
                     /gene="rpmH"
                     /locus_tag="ACHY09_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS3883802.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            36711..37070
                     /gene="rnpA"
                     /locus_tag="ACHY09_000791"
     CDS             36711..37070
                     /gene="rnpA"
                     /locus_tag="ACHY09_000791"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS3883803.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            37034..37291
                     /gene="yidD"
                     /locus_tag="ACHY09_000792"
     CDS             37034..37291
                     /gene="yidD"
                     /locus_tag="ACHY09_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS3883804.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            37294..38940
                     /gene="yidC"
                     /locus_tag="ACHY09_000793"
     CDS             37294..38940
                     /gene="yidC"
                     /locus_tag="ACHY09_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS3883805.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            39046..40410
                     /gene="mnmE"
                     /locus_tag="ACHY09_000794"
     CDS             39046..40410
                     /gene="mnmE"
                     /locus_tag="ACHY09_000794"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS3883806.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         9713 a        11029 c        10431 g         9317 t
ORIGIN      
        1 taaccataaa tttcatcaca ttagccgggc tgaataccag gaattgttag ccgtttcccg
       61 tggagacgct gttgccgatt atattattga taatgtctct attctcgacc tgatcaatgg
      121 cggagaaatt tccggcccaa ttgtgattaa aggacgttac attgcctgtg ttggcgcaga
      181 atacgctgat gctccggctt tgcagcggat tgatgcccgc ggcgcaacgg cggtgccagg
      241 gtttattgat gcgcacctgc atattgaatc cagcatgatg acgccggtca cctttgaaac
      301 cgctaccctg ccgcgcggcc tgacgaccgt tatttgcgac cctcatgaaa tcgtcaacgt
      361 gatgggcgaa gccggtttcg cctggtttgc ccgctgtgcc gaacaggcaa ggcaaaacca
      421 gtacttacag gtcagctctt gcgtacccgc cctggaaggc tgcgatgtta acggtgccag
      481 ttttaccctt gaacagatgc tcgcctggcg ggaccatccg caggttaccg gccttgcaga
      541 aatgatggac tatcctggcg taattagcgg gcagaatgcg ctgctcgata aactggacgc
      601 atttcgccac ctgacgctgg acggtcactg cccgggtttg ggtggtaaag aacttaacac
      661 ctatattgct gcgggtattg aaaactgcca cgaaagttat cagctggaag aaggacgccg
      721 gaaattacaa ctcggtatgt cgttgatgat ccgcgaaggg tccgcagccc gcaatctcaa
      781 cgcgctggca ccgttgatca acgaatttaa cagcccgcaa tgcatgctct gtaccgatga
      841 ccgtaacccg tgggagatcg cccatgaagg acatatcgat gccttaattc gccgcctgat
      901 cgaacaacac aatgtgccgc tgcatgtggc atatcgcgtc gccagctggt cgacggcgcg
      961 ccactttggt ctgaatcacc tcggcttact ggcacccggt aagcaggccg atatcgtcct
     1021 gttgagcgat gcgcgtaagg tcacggtgca acaggtactg gtgaaaggcg agccgattga
     1081 tgcgcaaacc ttacaggcgg aagagtcggc gagactggca caatccgccc cgccatatgg
     1141 caataccatt tcccgccagc cagtttccgc cagcgacttt gccctgcaat ttacccccgg
     1201 aaaacgctat cgcgttattg aggtcatcca taacgaattg attacccact cccgctccag
     1261 cgtctacagc gaaaatggtt ttgatcgcga tgatgtgtgc tttattgccg tacttgagcg
     1321 ttacgggcaa cggctggctc cggcctgtgg tttgctcggc ggctttggcc tgaatgaagg
     1381 tgcgctggcg gcgacggtca gccatgacag ccataatatt gtggtgatcg gtcgtagcgc
     1441 agaagagatg gcgctggcgg tcaatcaggt gattcaggat ggcggcgggc tgtgcgtgat
     1501 ccgtaacggt caggtacaaa gtcatctacc gttgcccatt gccgggctaa tgagcaccga
     1561 cacggcgcag tcactggcgg agcaaattga cgccttgaaa gccgccgccc gtgaatgcgg
     1621 tccgttaccg gatgagccgt ttattcagat ggcgtttctt tccctgccag tgatccccgc
     1681 gctgaaacta accagtcagg ggctgtttga tggcgagaag tttgccttca ctacactgga
     1741 agtcacggaa taataaaaaa aagcccggcg tcatgccggg caaaagtcac cagttaagtt
     1801 tatgccactg tcaactgctg aatttttttc tcgcggcgga ttttgcgttc ttccatcacc
     1861 gccactatcg ccatcaggca gatacaacca atcgcggcga tatccagcgc ggcgaaggtg
     1921 cctgcccagc cggtaaggcc aaataccggc gtcccatcgg caatcattcc cagacctaac
     1981 ttggcaaagc tgtcaccaat caggtaagcg aaggtgcctt taataccatc ggcagcgcca
     2041 atcgcttttt taggtacaaa gccaacagca gccacaccaa tcaacaattg cgggccaaag
     2101 accaggaaac ccaacgcaaa gagagaagcc aggtagatat attcgttgct ggcgtgttga
     2161 tacacaccga gcgtggcgat aatcagcgcc agcgcgatac aggccaccag gccacggcga
     2221 ccgttcgcca ggtcagagag ccagccccac agcagcgtac cgaccagcgc accagcttca
     2281 aacagcgtaa agctctgaat cgccaccgct ttagagagtt tcagttcctg gaacgcatac
     2341 acggttgacc actggtcgat accaatacgc accacataga ggaaaatgtt ggcgaagcac
     2401 agcagccaga tcactttgtt tttcagcaca tactcaacaa agatctgcca cttggtcata
     2461 tcggtagatt ctgtctcttt gtcctcttcg ctgatctcct cgccgaacag ctcttcagct
     2521 ttgccgaggc cataagattc cggggagtcg ctgccgtaac gcaggccgat aaaaccgaca
     2581 atcagcgcga taatcgacgg gaaaataaac atgccgatga catgaccatc gaacaggtaa
     2641 tttgcgccga acagcgccac acctgctgcg cctgcaccac caaggttgtg agaaatgttc
     2701 cagaaaccga ggaatgtccc gcgtttacga cgcggcgtcc atttggtgat ggtggagtaa
     2761 ctgcacgaac cgccggtact ctggaaaaag ccgctcaagg cgtagaaggc aatcatcagg
     2821 aacaggctaa ccgagccgct gcccatactg gcactgaagc ccagcataca aatggcggac
     2881 aggatcagca tgaacggcag gaattgtttg gtgtttttgc cgtcggcgta gtaagaaacc
     2941 agcgttttac ccacgccata agtgatggag aagcccaggc cgatcatccc cagctgcgtc
     3001 atgctcaacc cgtaggtcga gatcatgtcg ttctgcgcga tgttaaagtt cttgcgaatc
     3061 aggtacatcg taaggtagcc gataaagacc accaggtagg attgcatgaa cggtttgaac
     3121 cacattttgc gccgcacttc gagcggaagg tccagggtcg gcttgcgaac ctggtttagg
     3181 aaagccagca tgggttactc ctgatttccc ttcatacttc aagccgcagg tgccttagct
     3241 gcacgcattc ctcccggtca catagttcac tatgctcccg ggactcacgc gtttgctgcg
     3301 ttcctgcaac ttgaattatt ttgggaaaga attaatgaat gatggcattg taaaaatcag
     3361 cagcaaatca ggcctgagat agcgtccagg caaaacctgg aaaattgctt agttttgccg
     3421 gatatgaagt gaaaaggtga gatgcatcac gcttcgcgcg gtgtctgggc gttcaaaaag
     3481 ggcagtaaca gcagtgcgga aaccccggcg gcaatggcga taaccacaaa aaatccgctc
     3541 cagtgccagg tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa
     3601 gcaaacaagc cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acattctgcc
     3661 gccgccatgc cgattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag
     3721 gttgcctgca tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa
     3781 attccggcgg cgaaaatcaa attcatcggc ccgcggttgc cgttaaacaa tttgtccgag
     3841 ccccaacccg ctaccagcgc accgataaat ccacccagtt caaacatcgt cactgccgta
     3901 ttcgccgtga ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg
     3961 attgccgccc ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga
     4021 ttcagtaaca catatttggt gaggatctct ttacgcgtca gccctgcccc ttcttgttgt
     4081 tgagctatct ccagcgcgtc gtgccgccat tcaccaaccg ccggtaaacc taacgcctgc
     4141 gggcgatcgc gcagccgcca gcagagaaaa atccccacga ctatcgccat acaacccgca
     4201 atcatcatcc cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg
     4261 agtgcgccgc cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta
     4321 cgagaatacc aggcggttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag
     4381 gcgttcaaca cccaaagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg
     4441 ttgataatgc ccgtggcaat aagccctatc cccataaaat aacgggcatt tgagcgatcg
     4501 ctgacaatgc cggagacaaa cttcgacacg ccgtaggtaa tgtaaaacag ggtcgctaac
     4561 aggccaatat cgctacggct gagcacgccg ttagcaagga tttctggtac ggcggcgtta
     4621 aaacttttac gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc
     4681 cgacgccagt agcgatagcg ggcatcaatt tcatgtttat cagtcagtaa tggcgcatcg
     4741 gcaggcgctt tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa
     4801 acgctgacac gcgtgccgtg cagacaggaa atgtgtaatg tgccacccag cgccgttacg
     4861 cgctcgcgca ttccggtaag gccaaaaccg tgttgcccgg aatctggcgg taaaccgctg
     4921 ccatcgtctt caatcaccag cattaaccgc tcatcatgct gccagccttg cagcgtgacc
     4981 gcgctggcat cggcatgttt cacaatgttg ttcaaccctt cctggcagac gcgaaatagc
     5041 gtcacgcgct ggttttcgct taacgctgat tcatcgattc gccattcgag atgactgaca
     5101 atgccgcgcc cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg
     5161 agatcatcca gctggcgcgg acgtaaccgc cctaacaagc ggcgcaccgc gtcgtaaacg
     5221 cccagcgata gttgttcgat gagctgcccg ctctgtttca cgctggcgtt atccgccgcg
     5281 agccgctgga caatgcccgc ttgagttcga atagcagtaa tggtctgacc gatatcatcg
     5341 tgcagctcac gtgccacatc acggcgcacg ctctcttcgg tttccagcaa ccgttcagcg
     5401 agatgctgat tgcgcgccag ttccttttgc agcgactggt taagttcacg caaccgctgg
     5461 atgcctgcgc ccagtaacaa ccctgtcaga ctttgcacca gcagcgagag caataaatcc
     5521 accggatgat cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca
     5581 attagcgccc cttgccagcc atagtgccag gccagcgcga taatcggcag cgccaggcag
     5641 aatggcgtaa agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca
     5701 aacagcagca agtaccagac cagatgccgc ccacgccagt tgatcggttg agaaactaac
     5761 gacgggccga gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc
     5821 ggggccagcg tcaggccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg
     5881 tgccaaagcc agggcagcga ctgcaacaac gccgccgccg ttaatgccgc cccttgtagc
     5941 agcaaggtgc gccagtcacg ttgatgtcga tagcgcgaga tcagcgccac gggcagtaac
     6001 gtcagtaaac taccgatcat caataaggag aaatgggtta aaccgaccgc ctgcgttaac
     6061 cagtaaatca gcagccactc cgcgcccagc aacaccggcc agtatccgcg cgggcattgc
     6121 agcattagcc ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca
     6181 accaggtgca ggctgatact ccacaggcaa aaccatgcgg cagagaagat aaaaaagcag
     6241 gcaataacgg taattaagcg ggagaacaac gtcttcatca ccaaccatca aacatgcggc
     6301 gcgccagttc aacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt
     6361 gtaccgtttt cggcgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg
     6421 ccagtttttc tgccacctgg cgttcgcgtt tggttagcgg gtcctgacga ccggatgcca
     6481 gtttaatggc aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag
     6541 caatcagttc gtcagggcta caacgcttgg agagaaagcc gcgcgccccc gcgttaagcg
     6601 cctgctcaac cagcgccgga ctgtcatgaa cagagagcat aatcgtcgcc atacctttcg
     6661 gcagctggct tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca
     6721 cctgcacacc gcgccccggc aaccccgcca gcgcctcgcg ccccgaacca aactcggcaa
     6781 ctacctgcaa atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgataaggt
     6841 gatcgtctat aagggcaacg gtgatcatga tcttgtcctg gcgggtaata aaacgcgctt
     6901 accttaacga taagcgcgat gttgttcaag ccttgagcgg ttactgaaaa aacaccgcaa
     6961 tcttgttaaa cattgtcgga tcggactgat tacgctgcac tttcaccaca tcttccagct
     7021 tatcgatttg gcttatcatc tgctccagac gctggtcgtc attgaccagt agccagatat
     7081 ggcttttgtc gctgtcctga atcggcaggc agagaatgcc ttcaacgtta aaagcgcgac
     7141 gggcaaaaag gccacaaacg tgggtcatta cgcccggatg gttgcgaacg gtgagttcga
     7201 gaatcacgtt gtcatgagtt gtgttttgca tggcttattc ccccaccatt tcagtattcg
     7261 ccgcacctgg cggcaccatc gggtacactt tttcttcggc atcaatgcgc acgtggatca
     7321 gcgccggacc agggcgattg atgatttcct gcaatgcagc ctgcggatcg gtttcgttat
     7381 tcaaatcaca ggtttcgagg ccgaatccgg cggcaatctg cataaagttg atttttcccg
     7441 gataggtagc ggcaaaaacg ccctgcttgt agaacagact ctgttgctga tgtaccagcc
     7501 ccagcgcttc gttgttcatc agaatgattt tgacatccag ctgattttcg ctagcggtcg
     7561 ccatctcctg aatattcatc atcaggctgc cgtcgccgga gaaacacaac actttgcgat
     7621 ccgggttcgc aagcgctgcg ccaatcgccg caggcaggcc aaagcccatc gtgcccagcc
     7681 caccggaggt cagccactgg cgtgggcgat tgagcggata agcctgcgcc gtccacatct
     7741 gatgctgacc cacgtcggtg gtgataatcg cgctgtcatc gacacaggcg gcaacagcgt
     7801 tgatcaggcc gtaatgcgtt aacggatcgc acgctttcgg gattggacac gggaattcac
     7861 gctgcaaatc cgccaccaac tggtgccact ctgcacgcgg ttgcgcttcc accagcggga
     7921 tcaactgcgc caacacgtca tcaacatccg cctgaattgc cacatgcggt tgcttgattt
     7981 tgcccagctc tgcgcggtcg atatcgacat gaataatttt ggcattcggg cagaactgct
     8041 cggttttgcc aatcgcccgg tcatcaaaac gcgcgccgag aacaatcagc agatccgcct
     8101 cctgcaaaat atagttggtg ctgcgcacgc cgtgcatccc tagcataccc agcgacagcg
     8161 gatgcgcttt tggcaacata cccagcgcca ttaatgtcat tgtggtaggc agctgcgctt
     8221 tctccgccag ttcacgcact ctcgcaggcg cattgatcac accaccgccc agataaagca
     8281 ccgggcgttt ggcagcatta atcattgccg ctgcgtcacg aatgctttct tcgctaaagg
     8341 cgggcgcgat ggctttttct gccaccgcgg gctgtgcttc aatctcaaaa acggccgttt
     8401 gcacatcctt aggaatgtct atccacaccg ggccagggcg gcctgattgc gcaatacgga
     8461 aggcatcgct catgacctgc gggagttctt cgatatgtct gaccagatag ttgtgtttgg
     8521 tgatggggat agagatgccg taggtgtcga cttcctggaa ggcgtcggtg ccgatcatcg
     8581 aggcgggaac ctgaccagtg atgcaaatca gcgggatgga gtccagccgc gcatcggcaa
     8641 tggcggtcac caggttagtc gcccccggtc cgctacaggc catacagacc gccggtttac
     8701 cgtcggtgcg cgccattccc tgagcgataa accccgcgcc ctgttcatgg cgagccagaa
     8761 tatggcggat ttgcgtgctt tggcttaagg catcgtaaac aggcaggata gaaccgcccg
     8821 gaatgcccgt cacaatctta atgccctgct gttccaggaa atgaacgata aattctgcgc
     8881 cggtaaagcg cttacgcgtc gatgttgtgc ccgaacttgc catgctccag tccttttctt
     8941 ctgggccgac tttccgggag gtgcttaaac gaaaaacccc gcccggtttg cgccggcggg
     9001 gttttggaat cgtgtgttgt tccagtccct acggcgcatt gccgacgacc accaccacac
     9061 gcacgacgac cactgcggcg gatggcgcag ttggtagtag ttttgcgttg agcatggaag
     9121 tagtcattgg ggaccttgtt ggttttgtgt ttaacaatat ttatacaagc acagctttac
     9181 aggggagaca atggaaaatt tttcagcaag ggaaaattga ggggttgatc acgttttgta
     9241 ctgaattgca ggtaacaaaa aaccccgccg gagcgaggtt tcgtcagtcg cctgcggctg
     9301 gtaaccgcaa agcacactgt attatgtcaa cactgaaagt atacgtgttc cgcgcagaac
     9361 gcgcaatttc ggcacgaatt ttgacgtatt tagtgcatag ttgagtatcg atcacagttt
     9421 gcgttttgtc caaaattact gtttatttat acagtaaact tctataatat cactgtacgc
     9481 aatgtgttat gcgggggccg catcgttacc cggcgcacta agtcctggca gaaacgggtg
     9541 gtgccgtcag cgccttaacc ccgcgtgagc acactgtgtt atgtcaacaa gcacaacgtt
     9601 tctccttgag ataccgcgtg cacaacagct ggcaacaggc agcggaaagg tacgtcagct
     9661 ggcagtgctc ctgaaccaca ggagacgcgt atgagcctgg tggatatcgc cattcttatc
     9721 ctcaaactca ttgttgcagc actgcaactg cttgatgctg ttctgaaata cctgaagtaa
     9781 ttcagattca agtcgcacta aaggggagcg ggtaaccgct ccccttttat atttagcgtg
     9841 cgggttggtg tcggatgcga tgctgacgca tcttatccgc cctaccatct ctcccggcaa
     9901 catttattgc cgcttttgtt tacatattct gccgctaaac aattccccat tcctggcgta
     9961 tatctggcta acattcatca atgtgataga ttcctctccc gcatttatgg gaatgcgtag
    10021 tgacttattc taattatttt tataaaagca tccgtgataa tgaaaaggca tagaaacgtc
    10081 aatttgttat tgatgttggt attactcgtg gccgtcggtc agatggcgca aaccatttat
    10141 attccagcta ttgccgatat ggcgcgcgat ctcaacgtcc gtgaaggggc ggtgcagagc
    10201 gtaatgggcg cttatctgct gacttacggt gtctcacagc tgttttatgg cccgatttcc
    10261 gaccgcgtgg gtcgccgacc ggtgatcctc gtcggaatgt ccatttttat gctggcaacg
    10321 ctggtcgcgg tcacgacctc cagtttgacg gtattgattg ccgccagcgc gatgcagggg
    10381 atgggcaccg gcgttggcgg cgtaatggcg cgtactttgc cgcgcgattt atatgaacgg
    10441 acacagttgc gccatgctaa cagcctgtta aacatgggga ttcttgtcag tccgttgctc
    10501 gcaccgctaa tcggcggtct gctggatacg atgtggaact ggcgtgcctg ttatctcttt
    10561 ttgttggttc tttgtgccgg tgtgactttc agtatggccc gctggatgcc ggaaacgcgt
    10621 ccggtcgacg caccgcgcac gcgcctgctt accagttata aaacgctttt cggtaacagc
    10681 ggttttaact gttatttgct gatgctgatt ggcggtctgg ccggaattgc cgcctttgaa
    10741 gcctgctccg gcgtgctgat gggcgcggtg ttagggctga gcagtatgac ggtcagtatt
    10801 ttgtttattc tgccgattcc ggcggcattt tttggcgcat ggtttgccgg acgtcccaat
    10861 aaacgcttct caacgttgat gtggcagtcg gttatctgtt gcctgctggc tggcttgctg
    10921 atgtggatcc ccgactggtt tggcgtgatg aatgtctgga cgctgctcat tcccgccgcg
    10981 ctgttctttt tcggtgccgg gatgctgttt ccactggcta ccagcggcgc gatggagccg
    11041 ttccccttcc tggcgggcac cgctggcgcg ctggtcggcg gtctgcaaaa cattggttcc
    11101 ggcgtgctgg cgtcgctctc tgcgatgttg ccgcaaaccg gtcagggcag cctgggattg
    11161 ttgatgacct taatgggatt gttgatcgtg ctgtgctggc taccgctggc gacgcggatg
    11221 tcgcatcagg ggcagcctgt ttaagctcac gtcaccgcag catcgtcatc agctccatgg
    11281 gagaacgatg ctgctttatc agatcacgca tcacccgcat atgcggcgcg gagtaagaat
    11341 aaaacgcctg atagcccgca caaagcacgt tgtctggttt atcttcccga gcgtgcgggc
    11401 acagccgatg ccagtcgcac gcctggcagt cacggcgcgg ttgcgacttt tcgctgccac
    11461 accatttttg taaggtggtg tcaaaaagtt caatcgaaat acgactgata tctgcccgca
    11521 cccaacactc gaacagggca attaaaaatt tcccccacag ctctgctgtc accgaagcgg
    11581 ccgtcagatc gccccggtca tcaaattcaa gatggggaat aagctgtact gagcggatgc
    11641 ccagctgttt gaaattgtga taaaaatcgg caggttgcag agcatcctta tactctacaa
    11701 ccagtttatg cctgtcctct tcagcatcaa tgacctgact tcctgtcatg cgactctctc
    11761 aataaatacg ataagttgat gtatatgcgt tacagcaaac agtattgcga gagataacac
    11821 cgcgagggag atcaaaaatt tgtcacggac tacgtgaaat tgggaaaaat cgctattcgt
    11881 gacatccatt aaattgcgat tacgggtgta gtgccagagg atcagcgcca cgatggcgag
    11941 gatgccaatc gaaatccaga ataacatgcc cgcctggtgc cagttgtgtt tgatagccaa
    12001 cgccatcaac gcgccgtagc ccagcatggt acgaaaccac gccagtgatg tgcgttccgg
    12061 ctgcagccct gggtcggcaa tgcgtctggc tttgcggcta tccggcatac aacaccagtc
    12121 ccatgacgat cactgccacg accatcaata ttaaactgat gattaataag ctgttggtat
    12181 agggcaggtc ctctttcagg cgcatcgctt tttcattgcg taaccagcgc aagtagccat
    12241 acatcgctaa cccgccggaa aacagacaca acaacaacgc cagcagttcg cggatgacgg
    12301 gcgtggcgaa atctggcgca agttgatcaa gcccaacccc cgccgccaga aatcctagtg
    12361 cagtacggat ccacgccaga aaagtacgct cattagccag cgagaagcgg taatcgggcg
    12421 cttctccgag gcgggaaatt ttcattgttg ctccttgtaa actgttcctc gccgaaaaat
    12481 taggccagga acagcaataa tgcatcagaa cttctgtttt ttctcttcca ccttcacccc
    12541 ttgtgtcggt aaaccggtgt catagtcgcg taccactgga gaataaccat cctgtgggcg
    12601 tggacgaaac gcccccatcc agcgcggttg tgcatctttg cgccacggac gcaggttcca
    12661 ttgataactg cggaacggat cgcgaatttt gtccatataa tccaataagg cgtcatgcat
    12721 tttgctgcga acgtcggcaa aatggatatc atcgatcagg ttatgcattt catttgggtc
    12781 attacggcga tcgtaaagtt catcactggt gaagaggttg agcaccagtt taaagtcatc
    12841 cgttacccag caacgcaccg gaataaaacc gccaaagctg tcatgctcaa tctcgtagcg
    12901 attaaattcc accaacacgc cacgtggttc ttttacggca aggatattat cccccggcag
    12961 aatctctggt ttttcaatat ctgccagcgc catcattgtc ggcagtaagt caatatgact
    13021 gactggcgta tcgacctgtc gccgctcccc ttgcggcgaa cggatgatca gcggaatgcg
    13081 ggtgatgtcg tcatacatcg ccgccccttt actgatcagc ttatgtgcgc ccatcatttc
    13141 accatgatcg gaggtataaa tgacccacgt attttcacgt tgctctggcg ttaaggcatt
    13201 gatgacacgt ccgatttggt catcaacaaa gtcattgcag gcaaaataga gcggatggtg
    13261 ataaagcccg tcatcaccga ctggcgatgg catcgcctgc gcccataagc ggtgatgttc
    13321 cggtttgttc gccaggtcat cctgtgattt ctcgccaagt tcgtagtaaa aatcagcgta
    13381 tttctctaaa tactccaccg gacaggtgaa cgggtgatgc ggctcatcat acgaaaccac
    13441 catcaggaaa ggttcgtcgg cgcgcgcggg ctgttgcaga aaatctaccg cccgattgct
    13501 gatgcggtgc gcccaggtga aggtttcgtc gatatggttc gcctgtaaat cctcaacgct
    13561 gtttaggcca ttgcgccaca ggctgatctc tttttccgtc agttcgctaa ggtagttcgc
    13621 cccatcgaac cagtaatcag cgtcccactc cggcggacac tcgccagtgc cgaaatagtc
    13681 atgaccgtcg agatgccatt tgccaatgta acaggtgtga tagcccgcat ccttaaagta
    13741 gcgccccatg gtggaaatgt ttttacctgg cgcgacgttg ttggtccacg ggccggactg
    13801 gttagcgtag ataccggtaa acagcccggc gcgcgctggt gtacaaaccg gtgaacaggt
    13861 ataggcggaa ttaaagcgaa tactttccgc cgccagacta tcaatatttt gcgtattcag
    13921 cggcttaccg ctatagcaac cgaccatatt ggtggcctgg gtatcggtca tgatgaacag
    13981 aaaattgggg cgtttcatag gctatccttc gcgtcgggag taaaggttac ggctgggtcg
    14041 tgacgatgcc gccagctatc aaaaatcagg tagacaatca ctacggcggc aatgaaataa
    14101 ctgatcatcg ctaaccagcg cgtaccgtag ccgccgaatt cagccagccc ggcatagacg
    14161 ccaatcatcg cgaacaggat gcccattgac gcgatcttga cgtttttcca cggtttcatg
    14221 tccaccgcaa acgcatcttt gaaggtgaac ggcgtggcgc gcggtttgat aaaaccgatc
    14281 accagcatca cgaccacgtt gatgcagaac gtacaggcca gcacatagag gaaatggaag
    14341 tcgaacttca ccagatagtt gatggtgatg tagctgatta tgccaatccc catcgccact
    14401 tttgccgcca gcgccgggat gcgcgggaag aaaaagccca taatgatgat ggtaaccagc
    14461 ggcacgttgt aaatgccgtt gagctgtttc atccagctat acagcccctg cggcgcgttg
    14521 gcgatccacg gcgctaccag caccgaaacg atggcgataa agaaaccaaa tttgcgcccg
    14581 acggtgacca gctgctgcgg ctcggcattc tggttaatga tgcgccggta aatacccata
    14641 ctgaataacg tactggcgct attcagaaag ccgttgaagg tactgatcac cgcaccaaat
    14701 aacaccgcgc cgaagaaacc caccagtggc actggcagaa cgttattgac cagcgtcggg
    14761 taggccatgt cggctttcgg tagatcctga tacagatgaa acgcaatcaa ccctggcagc
    14821 accagtacca gcgggtcgag cattttcagc accgccgtta acagcgcccc cttttgccct
    14881 tccgccaggc tttttgacgc cagcgtgcgt tgcacgatgc cctgatttgt acaccagtaa
    14941 aaggtgttca ccagaatcaa accggtaaat gccgcgccaa tcggcaaggg atcggtcggg
    15001 ccgccgactg agtttaattt ctcggcgtga acggtggtga gttgctcaat gccctgcata
    15061 aagctgccct tgcccatcgc gatcaggcca aataccggca ccatcaaccc gccaataacc
    15121 agcccaatac cgttaatgga gtccgccact gccattgcgc gcagtccgcc gatcaccgca
    15181 tacaaaattc ccgccagacc aagcaaaata accagtaacc agatagccgc accatcggaa
    15241 agttgtagcg actcacccac atgaaacagg ctgttgagcg ccaacgcgcc ggaatagaga
    15301 acaatcggca gaaagcagac gccggtggca atgaggaagc agaagtcgat gataatacgc
    15361 gtcgttttat cataacgttc ctccagaaaa tcggggatgg tggcaatgcc gcgcttcaga
    15421 tagcgcggta aaaagattag cgcgaggaag atcagcgtca ctgcagaggt gacttcccag
    15481 cccatcaccg acatgccact tttgtaggcc tgcccagaaa ggccgaccag ttgttccgtg
    15541 gaaaggttgg ttagcattaa cgaagcggca ataaccggcg cttttagtga acgtccggca
    15601 agaaaatagc cttgttgcga tccggtatct gttttacgga ccttccacca ggtgatgacc
    15661 gctaccagca gcgtaaaacc gacaaaactc aagatttgta acgaattcat ctcatagccc
    15721 ttatttatgt ttatgtcccg gacgcatgaa tatagggaga ggtaaaatgc gtcgggtcag
    15781 gcagtcagat tcttaaaata caaacgtcgt atccctgaac ggattgtttt ctgttaagtt
    15841 caggttgtga gcataattcg ccggagggat attaaaatga atggaaaatt gcaaagttcg
    15901 gatgtaaaaa acgaaactcc gtacaatatt cccttattga ttaacgaaaa tgtgatctcc
    15961 agcggaattt ctctgatatc gctctggcat acctacgccg acgagcatta tcgggtgatc
    16021 tggccgcggg acaagaagaa accgcttatc gccaactcat gggttgcggt gtataccgta
    16081 caaggatgtg ggaaaattct tttaaagaat gacgaacaaa taactctgca tggcaactgt
    16141 attatatttt taaagccaat ggatattcac tcttatcact gtgaaggttt ggtctgggaa
    16201 cagtattgga tggaatttac ccccaccagt atgatggata ttcccgtcgg tcagcaaagc
    16261 gttatttata atggcgaagt ttataatcag gaactcaccg aagttgctga gttaattact
    16321 tcaccagaag caataaaaaa taatctggca gtcgcttttc tgacgaaaat tatttatcag
    16381 tggatttgtc ttatgcacgc agtcggtaaa aaagatccac aacggcggca aattgaaaaa
    16441 ttaattgcca ctttacatgc cagtctgcaa caacgctgga gcgtagctga tatggctgcc
    16501 acgatcccct gtagcgaggc ctggttgcgt cgtctgtttt tacgctatac cggcaagacg
    16561 ccgaaagaat attacctcga tgcgcgtctg gatctggcgc tatcgctatt aaagcaacaa
    16621 gggaactcgg ttggcgaagt cgctgatacg ctcaacttct tcgactcctt tcatttcagc
    16681 aaagccttta agcataaatt tggttatgcg ccgtcagctg tgctgaagaa tacggacagg
    16741 taataaccag aatggcgagt gtgtaaatga ctcgatgatg ttcagaaata ccaggtggag
    16801 taccatttac ggcgactcca cccggaaaag agttaaccga tactccagaa gagcaccgcc
    16861 agtaattggg gggtgataat gcgcaggaac atcaccaacg gatagacagt ggcgtaagag
    16921 agcgccgccg caccgctcgt tggatgaaga ttattagcaa atgccagcgc cggaggatcg
    16981 gtcatggagc cagccagcat cccgcacatg gtcaggtaat tcattttggc taacatccgc
    17041 gccagaatgc caacagtaat cagcggaacg gcggtgatca gggcaccata accaatccag
    17101 cttagccctt cgccatccac cagtgtatgg ataaaatcgc cgccggattt caggccaacc
    17161 accgagagaa agagcacaat gcctaactcg cgtagcgcca ggttagcgct tggtggcata
    17221 aaccagtaca gcttgccgat actgccgata cgcccgagga tcaacgccat aatcagcgga
    17281 ccgcccgcca gcccgagttt caacgccgcc gggaacccag gcacaaagac gggaatagaa
    17341 cctaacaata cgccaagccc gataccaata aacactggca gcatctgaac ctgttgcagt
    17401 ttttgctgcg cattccccag cacattggca acggcatcaa ttgcggacgg acgccccacc
    17461 aggttgagga tatcgccgaa ctgcaggctg atatcgctac ttgcaactaa ttcaacacca
    17521 gcacggttca ggcgcgagat aacaacgtca tagcgttctt taaagtgcag gtcgcgaata
    17581 cgtttaccga gcacgttttc attggtgacc accacacgct cgacgcgcaa gtcagtgcct
    17641 ttcgtagaca gcgaggtatc gacctcctga ccaatcacca gttgcgcatt atgtaaatcc
    17701 gcaggctgcc ccaccagatg cagcaaatcg cccagttgaa taacggtttc tggcgaaggc
    17761 acttttaggg tttcttctcg tttcaatcgt gagcaaataa ctttatcgcc attgagaatc
    17821 ggcacatctt taatggctaa attatgcaaa ttaggattct caactcggat gttaatcgtc
    17881 ctgattagcg caccgccgtt ggttcgcgtg gactcgtgct gctgggcctc agtctcaaca
    17941 ttgacgcgga aaataacccg cagcatccac atagtgaaca aaatgccgca aatcccaaat
    18001 ggatacgcca tcgcatagct catccccatt tgatcgacca tcgccatcgg tgtccccagg
    18061 tcgcgcaaga tctgctgccc tgcccccagt gctggcgtat tggttaccgc accggagaaa
    18121 atccccagta ctaccggcag tggaatatca aacagtttat gcaggatggc ggtaaccagc
    18181 cccccgatga tgacgatcag aacagcaaac aggttgaggc gtaatccgga gacgcgcaat
    18241 gaggcaaaga agcccggccc tacctgaatc ccgatggtat aaacgaacag gatcaggcca
    18301 aattcctgaa taacatgcag catatcgcta cttaatgtca tccccgcctg agaaacaaaa
    18361 tggccgacga tgatcccacc gaacagcacg ccgccaatgc ccaatccaac accgcgaaac
    18421 ttgacgttgc cgataaacaa cccgacgacc gccaccaaag ccagaatact gaccgttaat
    18481 gctatatcac tcatcgtcca tttctcttga gataacattt taattgctac ggattatggc
    18541 tgaatcggtg aagcagattt gccttatagc gcacattatc acgggaaaga acgtgccgaa
    18601 atatcttaaa caggcgaaaa ttaacgcatt gttgaagaag aaagacaaaa aaagccccac
    18661 cagaatggcg gggcaaagag aatagctagt tagctattta acgcgggacg ttcgctgata
    18721 gcgatacgct gcgctgcgat gggttcaggc tcattacgaa ttaaatcaat atgcagtaaa
    18781 ccgtttacga aggttgcacc agagacttcc atattttcag ccagcgtaaa gctcaggcta
    18841 aatggctgat tcataagccc ttgatgcagc cattttttct cttcttttgg ctgctccggc
    18901 gtgcctttta cgctcaggcg cgtaccttcc agttgaatct ctaaatcttc ctgacgaaaa
    18961 cctgccagcg caagggtaat gcggtagtgg ttatcgtcgc ttttctcaat gttgtacggc
    19021 gggaagctct ggctttcacc ggcgttttgc agtgcgttgg ccagtttgtc aaaaccgatc
    19081 cattgacgca tcagtgggga taaatcgaag ttacgcatag tcatttctcc ttctaagaag
    19141 cgagtaagta cctgcaaatc cgaagattcg catatgctcc ctgacggcga gcatggagat
    19201 gtcaggccgc gcaaggcggc cttagggaat tagttgattt cgatacggcg cggttttttc
    19261 gcttccggaa tcacgcgttc gagatcgata tacagcaaac catttaccag gttagcacca
    19321 cgaacatgaa tgttctcagc taactggaat ttgcgttcaa agttgcgttc agcgatgccc
    19381 tggtacagat aggtgcgctc tttttgttcg tcggcgtgag cacctttcac caccagcaga
    19441 ttatcctggg cggtaatttc cagttcgctc tcagcaaaac cagccacagc gatagcaatg
    19501 cggtaatggt tttcgtctac cagttcaacg ttatacggag ggtagccgcc attactctgg
    19561 ctctggttgt tttctaagtg gttaaacaaa cggtcaaatc caatagcaga acggtaaagc
    19621 ggggataaat caaagttacg cataatcaat agctcctgaa atcagcgaga atctaagacc
    19681 ttccacaatg gacaggtcag gtagccagaa cacccatcag gcgcgtcctc atcggctacg
    19741 atgtaaaaat gggtctggaa atgaactttt caagccttat cagcggactt ttttttgcag
    19801 tttatggtct attgcataga ctgagggggc agcacgaaac gttaaagtgc gatagccgcc
    19861 acagtgcgac gtaatgatga tgttattttc agcaagggat cgctataact catcaggcca
    19921 acacccacga tcaacagatg aatttattat gataagaaat gtgttgttag cgttcatgat
    19981 atgcagcgga atgacattac ttggaggatg ctccagcgtg atgtcccata ccggggggaa
    20041 agaaggaacg tatcccggca cccgcgccag cgctaccatg attggcgacg atgaaaccaa
    20101 ctggggcact aagtcgctgg cgattctcga tatgccgttt accgctgtga tggacacgct
    20161 tttactgccg tgggacgtgt tccgcaaaga cagctcggtg cgctcacgcg ttgaaaaaag
    20221 cgaagctaac gcgcaagcca ccaatgcggt tattccaccc gccagaatgc ctgataactg
    20281 atttagcgat cagtttcggt gatccacagc tggccgcctt ccatccacgc cagccactga
    20341 ccatccggcg agaagaccac ggcgtccgca gaaggtggat tggcgtggtt ttcggttaaa
    20401 tactcaacct cgccgctttg cgcatgggcg caagcaattc gattatccag cacaaagccc
    20461 aaccattctc ctgatggatg ccagttaaat gcagactgaa tatccgtttt gttatgggtt
    20521 aactggcgcg gctcgccgcc ctgtggcgag ataagccaca gttgcacaat gccgttatca
    20581 tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac agcgcaccca gtggcgcggg
    20641 acgttgacta accccggata agcccgatgg tgggtaaagg ttaaacgtcg ctgcacgacg
    20701 ccacgcggtg gcgcgggcag ggttgtttcc gttccactta acggcgcatc accagctgct
    20761 ttccagccag cttcatcttg cggtaactca acgataaata gctccggcac tttctcgccc
    20821 tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat ttcctaccca tccttcttca
    20881 taagcacgat tgatttcatt actgccaggc tgcggcgtgg gcgttgtttt actcaccagc
    20941 acgcaccagt ggctaccgct gtattcacgc ggatgctgct tttgtacgtt gaccgggcca
    21001 aacggcgcag caacacctac gtttcgcaaa tccagcgtcg aatcgagttg atgcattaca
    21061 tggtcgttat aggtaaagct caccctttct ccgttcgggc taaagacatg cacatggctg
    21121 ccgccgcgca gcgctcctgg ggtgtacggc gcggtaatat ccattgcatc gagattgctc
    21181 accttgccgc cttcagcaat cactccgcgc cgatgatgga agtcgtaata ccatgtttca
    21241 tcaggatttt ccgggccgtg gatgaaaaca tatttctctg acttcggatg aacggtcacc
    21301 acgccgacgt acgcgccctg tgacgcgcga tagataacct cgacctcgcc ggtatggata
    21361 ttcacacgct caatggtctc gccggtaaac gacgcccctg aaggacgcac gtcaaatacc
    21421 agccactggc tatcgggcgt ccaggtattg gtattggtga gcaggtgatt acggggagca
    21481 aaggttatct gtttcatggc gcggtcctga tacaaaacgg gtcccgccat catacttcac
    21541 aaggacttca ctttcagaca atagtctttt gccatcgtga tgaatgtgag tgaaaagatg
    21601 gaacatttcg acgtggcgat tattggcctc ggcccggcag ggtcggcgtt ggcgcgaaag
    21661 ttagcaggca aaatgcaggt gatcgcgctg gataaaaagc accagcatgg tactgaaggt
    21721 ttcagcaaac cttgtggcgg tctgctggca ccggacgcgc agcgatcttt tattcgcgat
    21781 ggactgacgc ttcctgtcga tgtcatcgcc aacccgcaga ttttcagcgt caaaaccgtc
    21841 gacgtcgccg catcgctcac gcgtaactac cagcgaagct atatcaatat taatcgccat
    21901 gctttcgact tgtggatgaa atcgctgatc cccgccagcg ttgaggttta ccacgacagc
    21961 ctgtgtcgaa aaatctggcg tgaggatgat aaatggcatg tcatttttcg cgcagacggc
    22021 tgggagcagc atattaccgc ccgctatctg gtcggtgccg atggtgccaa ctcgatggtg
    22081 cggcgacatc tctacccgga tcatcaaatt cgtaaatatg tcgctatcca gcagtggttc
    22141 gcagagaaac atccggtgcc gttctactcc tgcatctttg ataatgcgat aactgactgt
    22201 tattcatgga gtatcagcaa agacggttat tttatctttg gcggtgccta tcccatgaaa
    22261 gacggtcaga cgcgtttcac gatgctgaaa gagaaaatga acgcctttca gttccagttt
    22321 ggtaaggcgg tgaaaagcga aaaatgcacg gtgctgtttc cctcacgctg gcaggatttt
    22381 gtctgcggta aggacaatgc ctttctgatt ggtgaagcgg cgggatttat cagcgccagc
    22441 tcgctggagg ggattagcta tgcgctggat agcgcagaga ttctgcgttc ggtgttactg
    22501 aagcagccgg agaagagcaa cgccgcctac tggcgcgcca cccgcaaact gcgtttaaaa
    22561 ctcttcggca agatagtaaa aagccgatgc ctgaccgcac cggctttaag aaagtggatt
    22621 atgcgcagtg gtgtggcgca tattccacag ttgaaagatt agccaacgcg cttcacatcg
    22681 cccaccagca ggatgtaaga gagcgcgcca atcaacgcga cggcggagat ataaaccagt
    22741 gcaggtgcga aaccgtaacc ctgcgccagg taccccacca ccagcggaac ggtgatgccg
    22801 cccagaccac cggcgaagtt aaacacgccg ccggttaaac caatcaggcg catcggtgcc
    22861 agagaagaga ccagcgacca ggtaatcgaa gcaaaaccgt tgccgaagaa tgccagcgcc
    22921 atcaggcaca taatcatcat cggatcgttg gtgtagttag cgcccataat gcaggtggag
    22981 atcagcaagc cgcagataat cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt
    23041 accagcagat ccgcgaccca gccagagagc aggacgccga caaacgccgc gaggaatggc
    23101 accgtggtca taaagcctgc tttcagcgcc gtgattcctt tttcctgggt taaatagttc
    23161 gggaaccaag ttaagaaaaa ccacagtgta gaagccaccg caaattgccc aagatagacg
    23221 ccgatcagtt tacgatggaa caccagtttc cagtctttgg ctgttaacgg ctgacgcgcc
    23281 tcttttttca ccggcgcatc accatccacc agaccgccgc catcacgaat gtaatccagt
    23341 tcagctttgc tgatgccttt agtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc
    23401 gaccagataa tgccgatacc accggtgaca atgaacaccc agtgccagct caacatttcc
    23461 tgaatccaga tcagcagcgg agtcaaaaac gccagaccga caaactgacc agacgtataa
    23521 aaaccaacgg cggaagcgcg ttcatgttcc gggaaccagc tggtcaccat ccggttattg
    23581 gtcgggaaag cgggcgcttc gaaaataccg gttattgcgc gcagaccaat taatgacatc
    23641 aatcccgtgg caaagccctg gaataaagtc gccactgacc agccaaatat cgcaataaaa
    23701 taagtcacgc gtgaacctac gcgatctaaa aaccaaccgc cggggatctg acatagtgta
    23761 taaagccagg cgaaggccga aaatacatag cccatttccg ctttggtaat accgaactct
    23821 tcctgaatat gggcggaagc cacggccagg ttagcgcggt caacataaca aatgaccacc
    23881 gtaataaaga tcatcaccag cgtcagataa cgccgacgcc ccggctttgc tgcattaacg
    23941 ggaatatcca tagcgagctt tctccagatt ttgggcatag cgaagccgct caccatgccc
    24001 tgtaatttac agagggttat atttttgtat tgctgtttta gtgcccgatg agaggcttac
    24061 gtggcaggaa ttaccactct gctacgctgt tatcttcatg acgccagagc ggattacgcc
    24121 agtccggggc atttttactg aactcaataa ctttagcttc gtcgatttcc acacctaagc
    24181 ccggtttcgt taatggttta aagaaaccgc cgaccatgct gaagtcttct ttgtttttca
    24241 caaagtcgag taactccgcg cctttgttgt aatgaatgcc catactttgt tcctgaagta
    24301 cggcgttata ggaaacaaag tcgatatgca ggcaagccgc cagtgcaatc ggtccgagcg
    24361 gacagtgcgg cgcaagggtc acgtcatagg cttctgccat tccggcaatt ttgtagcatt
    24421 cggtaatacc gcccgcgtgg gagagatctg gttgcagaat cgaaatacca cccgcctcca
    24481 gcacacgttt aaaatcgaaa cgtgagaaca tgcgttcacc cgccgccagt ggaatatgcg
    24541 tttgtgccgc cagtttcggg tagtattcgg cttgttctgc cagcaccggc tcttcaataa
    24601 acagcgggcg atacggctcc agttctttaa tcagcacttt cgccatcggc gcgctgacgc
    24661 gaccgtggaa atcaagacca aactcaatct gattgccaaa agcttcacga atttgtgcca
    24721 cggtgttgac tgccgcatct accgcgcggg agttatcaat tagccccagt tcttcacaac
    24781 cgttcagttt gaaggtatcg aagccgattt cgcgcagggt tttaatgccg tcgataacat
    24841 ccgccggacg atcgccgccg acccaactgt aggctttaat tttgtcgcga accaggccgc
    24901 ccatcagttg ccagaccggc gcattcagca ctttgccttt gatatcccat aacgcctggt
    24961 cgatcccggc gatggcgctc atcaggattg gaccgccacg ataaaatccg gcgcgataca
    25021 tcacttgcca taagtcattg atgcgcgatg gatcctgacc aatcaaatag tcacccagct
    25081 cgtgaactgc cgcttccacc gtgcgggcgc ggccttcaat cactggctcg ccccagccga
    25141 ccacgccttc atcggtttca attttcagga acatccagcg gggaggtaaa cgatacgtgg
    25201 taattttggt gattttcatt gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg
    25261 ctgcgcggtg cgttctacgg actgcccggc gcgatagaga tcgctgccta agcccgcccc
    25321 tgcacaacct gcgtctatcc actgcgccag gttttctggc gtcacgccgc caacggcaaa
    25381 gactgcgatg tccggtggta ataccgcttt taacgctttg atgtattgcg gaccaaaagc
    25441 cgatgacgga aatattttca gcgcctgagc gcccgcgtcg agcgcggtaa aggcttcggt
    25501 cgccgtcgcg cagccggggc aaacggtcat gccgtagccc accgcacggc ggatcacttc
    25561 actatggata ttgggcgtaa cgatgagctg gcagctcatc ctggcgagtg catcgacctg
    25621 ttcaggtttc agtaccgtac ctgcgccaat caacgcctta tcgccatacg catcaacgat
    25681 ggcgggaatg cttttctccc attgagggga attcagcggg atttcaaccg cgtcgaaccc
    25741 ggcgtcaatc accgcgccaa catgcgccag cgcctcgtcg ggcgtaatgc cgcgcaaaat
    25801 ggcgatcagc gggagtttag tttgccactg catgagcgat gctccttata ccagcctgaa
    25861 atgccgtgtc gcccgccacc gccgtcacgt cgcaacccat cgcctgaaag gcttgctggt
    25921 agcgcgcggt cagcgatgtt ccggcgacaa gggtgatggc gtgttgatgg gtcacatagt
    25981 cgcgcatact ggcaacctct gcgccaatca acaaaccaga gagaaattcg ctgacctgtt
    26041 cgcggggaag tgttcccagc acatgcgagg cgcgaacttc aaaaagctgc ggcaatatgg
    26101 cgggagcatt aaagccacgc tcaagaccag ccgcgaaggc atcggcagag ttttcctgcg
    26161 gcggcaaacc tgcgccaatc aatgagtgat ttaacagtaa atgatgtaat tcaccggtca
    26221 tcacggtgcg aaaatcgttg atttgctggc tatcggcctg cacccatttg caatgggtac
    26281 cgggcataac ataaagagag gaaggagcga gagcacgcgc gccgatcaat tgtgtttctt
    26341 cgccgcgcat cacattgtgg ttatcgtcat gagagacgca taatccggga ataatccaga
    26401 tattgtcgcc aaccgacgtt aattgatcgc caatagacga aaaacgggca ggaacagata
    26461 aatacggtgc aactttccag ccgacattgc tgccgaccat tcctgccatc accactggcg
    26521 tattctcttc acgccagtcg gtcgtgactt ctgctaacac cgcagccgga gattttccgt
    26581 tcaggcgcgt gacgcctgct tctgattgcc tgctgtccag gcagtggtcg ccctgataaa
    26641 gccaggcgcg cagattggtc gatccccagt caattgcgat gtagcgagct gtcatgtgat
    26701 ttcctttaac cttcgtgtcg agctggcgat catggtaagc gccgcctgct ctgccgcatc
    26761 gccgtcctga tgccgtattg catcgaacag cgccttatgt tcctggagcg tttgcggcat
    26821 gttggcctca tcgcccatcc aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc
    26881 aatgctaagt tgctgtaaca ccgggttatg caccgactgc agcaccgcct cgtggtagcg
    26941 aatatccgct tcgttaaacg cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc
    27001 cgattcaatc tgcgccagat cgctggaggt cgcgcgctct gctgcccaac gggcaatagc
    27061 cggctctacc aggtttcgca cttcgctcat tgcactgata agccgtgggt catagtcgtt
    27121 ttccagcacc cattgcagta cgtcagtgtc gaggtaattc cactggttac gcggagccac
    27181 aaacgccccg cgataacgtt tcatttcaat cagccgcttc gccatcagcg aacgaaacac
    27241 ctcacggatg atgttgcgcg aggttgcaaa ctcttcacag agctccgcct cagccggaag
    27301 tggagagcct ggtacgtatt tgccgtgaac gatctgttta cccagcgtaa taacgatgcg
    27361 atcggtttta ttgagagtca tggagagtcc ttgtgctctg tatgttcttc tctactttac
    27421 cccgatcgat gcataacgcg gcaactttgt agtaccagcg tgatgacgtt cgcgtttgct
    27481 gtgcgcctaa tgtagtacaa acttatattg ttgtactaca atttagatca caaaaagaac
    27541 aatacataaa aaatgacatg cgtcgggcag aaatctgaaa agggatgtca ggcgctaaac
    27601 aggagggaaa gaagagtatg ctttcaacgg cttagctact cgtttaaagg attaatcatg
    27661 aagttgaatt ttaagggatt ttttaaggct gccggtttgt gttctttggc atttgcgttg
    27721 accggatgta tcacctgggg attggtttcc aatactgcaa gccgtccaca tgccgagcaa
    27781 tggcggtcag acacgattaa gggtttatcg ctggctgaag acagtaacgg tacgaaaggc
    27841 tatgtttttg ttggtgaatc gttggactat ctgttgacca ccggcggcga tgaggtggtt
    27901 aaaatgctga acgatccggc aatccatggt gaaagaatca ccgtttctga taacgcgaaa
    27961 tttattttaa gcagcagtaa taagaatttc tctggtgcta ttacgctcta ttacgactgg
    28021 aacaacgaag aagataaggc actggcaaca caatatggtt ttatttgtga tacccggcgt
    28081 tgtacatgga tgctggacgg tttgaaaggc tcaatccacc agaaaaataa aaaagcggat
    28141 tactcaaacg tgatggtttt ccatcaaccc tttacggtgg gattttatga gtacaaagca
    28201 acagatggcg tcccgcgcgg cctcgttaat gcgctgctgc cagtcactct tacgctggat
    28261 atagtaacgt caccactcca gttcctgatt ttatgcacga ccagaaattg ttaatcgctg
    28321 cgggcaaacg cattcatgcc gtttgcccat tcactaatta attcagcaca tacttctcaa
    28381 tagcaaatgc cacgccatct tcaaggttgg atttggtgac aaagttcgcc acttctttca
    28441 ccgaaggaat agcgttatcc atcgccacac cgacgcctgc atattcaatc attgcgatgt
    28501 cgttttcctg atcgccaatc gccatgattt cttccggttt aatacctaac acgtcggcca
    28561 gtgatttcac acccgtacct ttgttaacgc gtttatcgag gatttcgagg aagtacggcg
    28621 cacttttcag cacggtgtat ttttctttca cttcctgcgg aatacgcgcg atggcctggt
    28681 cgaggatggc aggttcatca atcatcatca ctttcaggaa ctgggtattg gggtccattt
    28741 tctccgcttc gcagaacacc agcggaatgg tggcaacgaa agattcatgc accgtgtagt
    28801 agctgatatc acgattggcg gtgtacagcg tggtgcggtc cagggcgtgg aaatgagaac
    28861 cgacttcgcg agagagtttt tccaggaaac ggtagtcgtc atagctgaga gtggtttgcg
    28921 ccacggtgct accatcagcg gccttctgta ccagcgcgcc gttataagta atgcagtagt
    28981 cgcccggctg ttccatatgc agctctttca ggtaattgtg cacacctgca taagggcgac
    29041 ccgtcgttag cacgacattc acgccacggg cgcgagctgc ggcaatcgca tttttaacgg
    29101 cgggtgaaat ggtgtgatcg ggcagcagaa gggtgccatc catatcgata gcaatgagtt
    29161 tgatagccat gagttcccca ggtagattgg ttcctgaccc atgctaacgc gattccgctc
    29221 aaaaatcagt acaacacccg agggaaaagg gggatgcaac gcgcgttgcg tgctcccctt
    29281 ttgcttagcg gaagagtttc cctttcagca gttccatgcc tgcggaaagc agatcgttat
    29341 tggcttgtgg tgacacttca ccttgcggtg agagtgcatc aataatcttc ggcaattgtt
    29401 ctgccagtaa actggaagct gtactggtat ccacgcccag tttttgcccg agatcggaca
    29461 ccgcatttgt gccgagcgcc gattccagtt gctcgccgct aactggttgg ttgccctgtt
    29521 gattactcag ccaggttgag agaatggcac ctaaaccgcc actttgcagt ttttccagca
    29581 gcacctgaat gccgccctgc tcctcaaccc aacttaaaat cgcctgatat ttccccgcat
    29641 cgcctttcag aaaggcgccg acaacgtcat caaaaaggcc catgataatc acctgtaaag
    29701 cgttacgtgt tgactcaaaa agtatagttc tgtgggatga taattgcgga ttgcaggaat
    29761 aaaaagggcg gagatgatct ccgccctttt cttatagctt cttgccggat gcggcgtgaa
    29821 cgccttatcc ggcctacaaa aacatgcaaa ttcaatatat tgcaggagtc tcgtaggcct
    29881 gataagcgta gcgcatcagg cacgctcgca ttgttaacgc cattaaatat caatattcgc
    29941 cgctttcaga gcgttctctt caataaacgc acggcgcggt tcaacggcgt cgcccataag
    30001 cgtagtgaac aactggtcgg cagcaatcgc atctttaacg gtaacgcgca gcatacgacg
    30061 gctttccggg tccatggtgg tttcccacag ctgttccggg ttcatctcgc ccagaccttt
    30121 ataacgctgg atggagaggc cgcgacggga ctctttcacc agccagtcca gcgcctgctc
    30181 gaagctggct accggctgac gacgttcgcc acgttcgata aatgcatctt cttccagcaa
    30241 gccacgcagt ttctcaccca gcgtgcagat acgacgatat tcgccgccag tgataaattc
    30301 gtgatccagc ggatagtcag tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa
    30361 caggttttgt tcggcattgg tgtggacatc aaacttccac tggctgccgt gctgttcttt
    30421 gtcgttcagt tcgctgacca gcgcgttcac ccagcgggta acggtctgct catcagagag
    30481 gtcggcttcc gtcagcgtcg gctgatagat aagctctttc agcattgctt tcggataacg
    30541 acgctccatg cggttgatca ttttctgcgt cgcgttgtac tcagacacca gtttctctaa
    30601 cgcttcgcca gccaatgccg gtgcactggc gttggtgtgc agcgttgcgc catccagcgc
    30661 gatggagatc tggtactgat ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc
    30721 tttcttcact ttgtacagcg gcggctgagc gatgtagacg tgaccgcgtt caacgatttc
    30781 cggcatctga cgatagaaga aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc
    30841 cgcatcggtc atgatgatga tgctgtgata acgcagtttg tccgggttgt actcgtcacg
    30901 accgatacca caaccaagcg cggtgataag cgtcgccact tcctgggaag agagcatctt
    30961 atcgaagcgc gctttctcga cgttgaggat tttacccttc agcggcagaa tcgcctggtt
    31021 cttgcggtta cgcccctgct tcgcagagcc gcccgcggag tccccttcca ccaggtacag
    31081 ttcagagagc gccggatcgc gttcctggca gtctgccagt ttgcccggca ggcccgctaa
    31141 gtcgagcgca cctttacggc gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg
    31201 ggcagcatcg ataattttgc caaccacgat tttcgcgtcg gttgggtttt ccagcaggta
    31261 ttctgccagc agttcgttca tctgctgctc aaccgccgat ttcacctcag aagaaaccag
    31321 tttgtctttg gtctgggagg agaatttcgg gtccggcact ttcacggaaa cgaccgcaat
    31381 caggccttca cgcgcatcgt caccggtggc gctgactttg gcttttttgc tgtagccttc
    31441 tttgtccatg taggcgttca gggtacgggt catcgccgca cggaagcctg ccaggtgagt
    31501 accgccgtca cgctgcggaa tgttgttggt aaagcagtag atgttttcct ggaagccatc
    31561 gttccactgc aacgccactt cgacgccaat accgtctttc tcggtggaga agtagaagat
    31621 attcgggtgg atcggcgttt tgttcttgtt cagatattca acgaacgcct tgatgccgcc
    31681 ttcatagtgg aagtggtctt ctttgccgtc gcgcttgtcg cgcagacgaa tggaaacgcc
    31741 ggagttgagg aacgacaact cacgcagacg tttcgccaga atttcatatt cgaactcggt
    31801 cacattggtg aaggtttcga ggcttggcca gaaacgcacc atggtgccgg ttttttcagt
    31861 ctcgccggta accgccagcg gggcctgcgg tacaccgtgt tcgtagatct gacggtgaat
    31921 tttaccctcg cgctggataa ccagctccag tttttgcgac agggcgttta ctaccgaaac
    31981 accaacgccg tgcagaccgc cggacacttt ataggagtta tcgtcaaatt taccgcctgc
    32041 gtgcagaacg gtcatgatca cttccgccgc cgatacgccc tcttccgggt gaataccggt
    32101 cggaatgccg cgcccgtcat cctgtacaga gacagagtta tcggcgtgaa tggtgacgat
    32161 aatttcttta cagtgacccg cgagcgcttc gtcgatagcg ttatctacca cctcgaatac
    32221 catgtggtgc agaccggtgc cgtcatccgt gtcgccgata tacatacccg ggcgcttacg
    32281 caccgcatcc agccctttca ggactttgat actggaggag tcataagaat tcgacatcaa
    32341 cgtttctcgc tcatttatac ttgggttaat ccgttatttt acccttttcc acggtaaaca
    32401 tcttcgaatt ttcgtccgac atgtctataa cgtgttcagc actgatcgcg ctgacaaaga
    32461 cctgtgattg cgtcgctttt aagcggctgg caagcagccc gcgacgctca tcatcaagct
    32521 cagaggcaaa atcatctatc aggtagagac accgccgccc gctttcacgg gtgaggaact
    32581 ctccttgcgc cagacgtaag gcgcacatca acagcttaag ctgcccacgc gataaggtat
    32641 cttccaccgg cgcaccgtcg gcgcgaatgc gtaagtccgc tttatgcggg ccgtgcgcgg
    32701 tgtaggttag ctggcgatcg cgttcaaaat tacgttccag cacctcagcg tactctgtct
    32761 ctttctccca gccgcgctgg aaagagaaag tcagagaaaa ctcagggaga aattgcttac
    32821 aggtatcggc catatcagcc gcgataccgg cgctatactc cgcgcgccag gtgcttattt
    32881 gctccgccag cggaatcagt tctttatccc acgggcgtag ctgttcgtaa cgtgtcacct
    32941 ggcgcagcgc cgcattgcgc tgcttgagca atcgcttgag attgctccag gcggtgaaaa
    33001 atccgggttc gttgtgaaag catccccagt cgaggaatgc tcttctgtat ttggggccgc
    33061 cgttgagtaa agtaaaccct tctggcgtta tcaactgcat tggcatcagg tgcgccagtt
    33121 ccgcgacctt atgcccgtct gtaccgtcga tgcggacttt gctgtcgccc tgtttgtctt
    33181 tggttaagcc aatcgccgtc tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaacg
    33241 cctcctgctc gtggcgaatg acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc
    33301 cgagcgtata gatggcttcc agcacgctgg ttttgccact accgttggca cctaccagaa
    33361 agttaaagcc gggagataaa gcgagatccg cggtttcgat attgcggaaa tcgcggatca
    33421 acaagcgggt gagggacatt acagtctcat tggcatgaca acataggcag ccgactgtga
    33481 tgcggcatct tcaatctgca cgctggaaac cgaatcggtc agcatcatgc ggacgttttc
    33541 gcatttcagt gcgttcagaa catccagcac atagctgacg ttgaagccga tttccatctc
    33601 cgcaccgcta taggtaacgt cgaggatctc ttccgcttct tcctgttctg ggttgttggc
    33661 ggtgattttc agctggtttt cgctgacata cagacgcacg ccgcggaatt tctcgttaga
    33721 gagaattgcc gcacgggcaa acgcctgctt gagcagatca cagccagctt ccagatgctt
    33781 gtccgggttc ttcggcagaa cgcgacggta atccgggaag cgaccatcca ccagtttcga
    33841 ggtgaagata aagtcgccaa cgtgggcgcg aatgttgttg ctgccaatct gcacgcgcag
    33901 cggattgtcg ccgccgtcga gcatacgcat cagttcaatc acgcctttac gcggcacgat
    33961 caccgaatgg cttggcaaag attgaccaat tggcattgaa cagactgcca gacggtggcc
    34021 gtcggtcgcc acggtgcgca gttcttcacc ttcggtttca aacagcatac cgtttaagta
    34081 atagcgaacg tcctgatgcg ccatagaaaa ctgggtcgct tcaatcagac gcttcatcgt
    34141 cgcctgcggc agggtaaatt cgacttcgct ctgccagtca tcgaggttcg ggaaatctgc
    34201 cgctggcagg gtagacagcg aaaaacggct acgcccggag cgcaccagca tccgctcacc
    34261 ttccagctgc acggcaattt ccgcgccttc aggcagacca cggcagatat caaagaattt
    34321 gcgcgccgga acggtcgtcg cccctggttc gtgcggctga accagcgcaa cgcgtgccac
    34381 catttccatc tcgagatcag taccggtcag cgacaacgta ccgtcagcaa cctgtaacag
    34441 cagattaccg agaatcggta gcgtaggacg accacctaac ggaccgctca cctgttgtag
    34501 cggttttaat aaatgctcac gttctacggt aaatttcata ggtttacgat gacaatgttc
    34561 tgattaaatt agaaaaatct tctttgatat cgtggctctc ttcacgcaac tgctcgatct
    34621 tccggcaggc atgaagcacc gtcgtgtggt cacgaccacc aaaggcatcg ccaatctccg
    34681 gaagactgtg gttagtcagc tctttcgcca gcgccatcgc catttggcgc ggacgggcca
    34741 ccgagcggga tcgacgcttg gaaaggagat ccgcgacttt gatcttgtag tactccgcca
    34801 ccgtcttctg aatattgtcg atggtgacca gtttttcctg caatgccagc aagtcgcgca
    34861 gcgcctcacg cacgaagtcg atggtgatcg cccgtccggt aaagttggca ttggcaatga
    34921 cgcggttcag cgcgccttcc aactcacgta cgttagatcg tagacgcttg gcgataaaaa
    34981 acgccacttc gcccggcaaa cgaatgtcgt tttcgtcggc ctttttcatc aggatcgcca
    35041 cgcgggtttc cagctctggc ggttcgatcg ccacagtcag tccccagccg aagcgggatt
    35101 tcaaacgatc ctcaacgccg ttgatctctt tcggatagcg atcggaggtg agaatgatct
    35161 gttgattacc ttccagcagg gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt
    35221 tattagcaaa aaactgaata tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt
    35281 taaactcttc gatcgcgttg ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg
    35341 agtgcatata aaccactttg gcattcggct tgcgcgccat aatgccgtta cccaccgcat
    35401 gcagcaggtg agttttaccc aaacccgtgc cgccataaag gaacaacggg ttataagcac
    35461 cgccagggtt atctgccacc tggcgagccg ccgcgcgcgc cagttggtta gatttacctt
    35521 caacgaagtt atcaaacgtg tgtttgacgt ttacgttaga acgataggtc ggttctgccg
    35581 gagccgggac gttatcccaa cccgagcgcg tagaaggcgc agcacgttgc ggctgcgttt
    35641 gcgccacctg tgcaggggcc gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca
    35701 ccggtttggt gccgacttca aaacgcagct gtggggcatc cgctccgcag aaactggtta
    35761 gcagtccatt gatattatta aggtacttgt cccgtaccca atcgaggaca aaacggtttg
    35821 gcgcgtacag ggccagcgtg ttatcgctca gttccgcctg caatgggcgt atccacatac
    35881 tgaattctgt ggctggtaac tcatcctgca atcgggcaag acactgctgc caaagcgaaa
    35941 gtgacacggc ggactccact cgaacaaaag tcgataatga ctaaggctga aacattcatg
    36001 attgttgacg tacgtcgaaa agaccctgca tgagggtgac gcacgaaccg ctgtctgcgg
    36061 ttatatgccc gatcaagatc ctgcaaaacg atcgggaccg cggatcatag cctaaactgc
    36121 gcaagagatc ttctgtttct cacagatttt cccgatttat ccacaggact ttccagaact
    36181 cgctaagtgt aaacgatcct gccgcgggcc gggcacgatt tacgccgcat attggaaaat
    36241 ttaatgacca tagacaaaaa ttggcttaat cgatctaata aagatccagg acgatccttg
    36301 cgctttaccc atcagcccgt ataatcctcc acccggcgcg ccatgctggt tttcactggt
    36361 gtgaggccgt acattttccc tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag
    36421 aattgactcc ggagtgtaca attattacaa tccggcctct ttaatcaccc atggcttcgg
    36481 tgtccatcgt ttcatttttc ggcggatatc caataaagcc attgaattta ttcaagttta
    36541 ggtagaaatc gccatgaaac gcacttttca accgtctgta ctgaagcgca accgttctca
    36601 cggcttccgt gctcgtatgg ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc
    36661 taaaggccgc gctcgtctga ccgtttctaa gtaataaagc taacccctga gtggttaagc
    36721 tcgcatttcc cagggagtta cgcttgttaa ctcccagtca attcacattc gtcttccagc
    36781 agccacaacg ggctggcacg ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc
    36841 atccccgtat cggtcttaca gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc
    36901 ggattaaacg tctgacgcgt gaaagcttcc gtctgcgcca acatgaactc ccggctatgg
    36961 atttcgtggt ggtggcgaaa aaaggggttg ccgacctcga taaccgtgct ctctcggaag
    37021 cgttggaaaa attatggcgc cgccactgtc gcctggctcg cgggtcctga tagccctcat
    37081 tcgggtctat caacgcctga ttagtccgct actcgggccg cattgtcgtt tcactccaac
    37141 ctgttcaagc tacggaattg aggcattgcg caggtttgga gtgataaaag gcagttggtt
    37201 gacggtgaaa cgcgtattaa aatgccaccc tttacaccct ggtggtgacg atcccgtccc
    37261 gcccggacca tttgatacca gagaacacta acgatggatt cgcaacgcaa tcttttagtc
    37321 atcgctttgc tgttcgtgtc tttcatgatc tggcaagcct gggagcagga taaaaacccg
    37381 caacctcagg cccaacagac cacgcagaca acgaccaccg cagcgggtag cgccgccgac
    37441 cagggcgtac cggccagtgg ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat
    37501 ctgaccatca acacccgtgg tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa
    37561 gagctgaact ctacccagcc gttccagctg ctggaaactt caccgcagtt tatttatcag
    37621 gcacagagcg gtctgaccgg tcgtgatggc ccggataacc cggctaacgg cccgcgtccg
    37681 ctgtataacg ttgaaaagga cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg
    37741 ccgatgacgt ataccgacgc ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt
    37801 ggtgattacg ctgtcaacgt caactacaac gtgcagaacg ctggcgagaa accgctggaa
    37861 atctcctcgt ttggtcagtt gaagcaatcc atcactctgc caccgcatct cgataccgga
    37921 agcagcaact tcgcactgca caccttccgt ggcgcggcgt actccacgcc tgacgagaag
    37981 tacgagaaat acaagttcga taccattgcc gataacgaaa acctgaacat ctcttcgaaa
    38041 ggtggttggg tggcgatgct gcaacagtat ttcgcgacgg catggatccc gcataacgac
    38101 ggtaccaaca acttctatac cgctaatctg ggtaacggca tcgccgctat cggctataaa
    38161 tctcagccgg tactggttca gcctggtcag actggcgcga tgaacagcac cctgtgggtt
    38221 ggcccggaaa tccaggacaa aatggcagct gttgctccgc acctggatct gaccgttgat
    38281 tacggttggt tgtggttcat ttctcagccg ctgttcaaac tgctgaaatg gatccatagc
    38341 tttgtgggta actggggctt ctccattatc atcatcacct ttatcgttcg tggcatcatg
    38401 tacccgctga ccaaagcgca gtacacctcc atggcgaaga tgcgtatgct gcagccgaag
    38461 attcaggcaa tgcgtgagcg tctgggcgat gacaaacagc gtatcagcca ggaaatgatg
    38521 gcgctgtaca aagctgagaa ggttaacccg ctgggcggct gcttcccgct gctgatccag
    38581 atgccaatct tcctggcgtt gtactacatg ctgatgggtt ccgttgaact gcgtcaggca
    38641 ccgtttgcac tgtggatcca cgacctgtcg gcacaggacc cgtactacat cctgccgatc
    38701 ctgatgggcg taacgatgtt cttcattcag aagatgtcgc cgaccactgt gaccgacccg
    38761 atgcagcaga agatcatgac ctttatgccg gtcatcttca ccgtgttctt cctgtggttc
    38821 ccgtcaggtc tggtgctgta ctatatcgtc agcaacctgg taaccattat ccagcagcag
    38881 ctgatttacc gtggtctgga aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga
    38941 ttcggtgagt tttcgctaaa ataagggcgg tcagttgacc gccttttttc ttttcgtagg
    39001 gcggataagc accgcacatc cgccacacaa agcaacagga acatcatgag cgataatgac
    39061 actatcgtag cccaggccac gcctccggga cgtggcggcg ttggcatcct gcgcatctcc
    39121 ggcttcaaag cccgtgaagt tgccgaaacc gtgctgggta aactgcctaa gccgcgctac
    39181 gccgattatc ttccgtttaa agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta
    39241 tggttccctg gcccgaactc gttcaccggc gaagatgtgc tggaactgca aggtcatggc
    39301 ggtccggtga tcctcgacct gctgttaaaa cgcattctga ccattcccgg cctgcggatt
    39361 gctcgcccgg gtgagttttc cgaacgcgcg tttcttaacg ataaactcga cttagcccag
    39421 gccgaggcga ttgcggatct tatcgacgcc agttcggaac aggcggcccg ttcggcactt
    39481 aactcgctgc aaggcgcatt ctccgcacgg gttaatcatc tggtagaagc cctcacccac
    39541 ttgcgcattt acgttgaagc ggcaattgat ttccccgatg aagagatcga tttcctctcc
    39601 gacggcaaaa ttgaagccca gcttaatgac gtgattgccg atctcgatgc agtgcgtgct
    39661 gaagcacgtc agggtagttt gttgcgcgaa gggatgaaag tggtgattgc cggacgtcct
    39721 aacgccggta aatcgagcct gttaaacgcg ctggctggcc gtgaagcagc aatcgtaacc
    39781 gatatcgccg gaactacgcg tgacgtgttg cgtgagcata tccacattga cggaatgccg
    39841 ctgcatatca tcgacaccgc cgggctacgt gaagccagcg atgaagtgga acgtattggt
    39901 atcgagcgcg cgtggcaaga aattgaacaa gccgaccgcg tgctgtttat ggtcgatggc
    39961 accacgacag acgccgttga tccagcagag atctggccgg agtttatcgc ccgtctgcca
    40021 gcgaaactgc cgatcaccgt ggtgcgcaat aaagccgata tcaccggaga aacgttgggg
    40081 atgagcgaag tgaacggtca cgcgttaatt cgtctctctg caaggactgg tgaaggtgtg
    40141 gacgtgctgc gtaaccatct caaacagagc atgggctttg acaccaacat ggaaggcggc
    40201 ttcctggcgc gtcgtcgtca cctacaggca ctggaacagg cggcagagca cctgcaacag
    40261 ggtaaagcgc aactgttagg agcctgggca ggcgagctgc tggcggaaga actgcgtctg
    40321 gcgcagcaga acttaagcga gatcaccggg gaatttactt ctgacgacct gctggggcga
    40381 attttctcca gcttctgtat tggtaagtaa ccgcgcttgc gaagccgcat tctgactgtc
    40441 agatgcggct tcgcttcatt gttagcactc ctgttactcc tcaacccttt
//
LOCUS       DAZUCC010000017        38374 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZUCC010000017 DAZUCC010000000
VERSION     DAZUCC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.017"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            77..1732
                     /gene="aslA"
                     /locus_tag="ACHY09_000795"
     CDS             77..1732
                     /gene="aslA"
                     /locus_tag="ACHY09_000795"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFS3883807.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1890..3125)
                     /gene="aslB"
                     /locus_tag="ACHY09_000796"
     CDS             complement(1890..3125)
                     /gene="aslB"
                     /locus_tag="ACHY09_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFS3883808.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKVFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3272..3348)
                     /locus_tag="ACHY09_000797"
     tRNA            complement(3272..3348)
                     /locus_tag="ACHY09_000797"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3312..3314),aa:Pro,seq:tgg)
     gene            complement(3391..3477)
                     /locus_tag="ACHY09_000798"
     tRNA            complement(3391..3477)
                     /locus_tag="ACHY09_000798"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3441..3443),aa:Leu,seq:cag)
     gene            complement(3498..3573)
                     /locus_tag="ACHY09_000799"
     tRNA            complement(3498..3573)
                     /locus_tag="ACHY09_000799"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3538..3540),aa:His,seq:gtg)
     gene            complement(3632..3708)
                     /locus_tag="ACHY09_000800"
     tRNA            complement(3632..3708)
                     /locus_tag="ACHY09_000800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3672..3674),aa:Arg,seq:ccg)
     gene            complement(3811..5196)
                     /gene="yifK"
                     /locus_tag="ACHY09_000801"
     CDS             complement(3811..5196)
                     /gene="yifK"
                     /locus_tag="ACHY09_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3883809.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEK"
     gene            complement(5387..6127)
                     /gene="wecG"
                     /locus_tag="ACHY09_000802"
     CDS             complement(5387..6127)
                     /gene="wecG"
                     /locus_tag="ACHY09_000802"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFS3883810.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6130..7482)
                     /gene="wzyE"
                     /locus_tag="ACHY09_000803"
     CDS             complement(6130..7482)
                     /gene="wzyE"
                     /locus_tag="ACHY09_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFS3883811.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7479..8558)
                     /gene="rffT"
                     /locus_tag="ACHY09_000804"
                     /gene_synonym="wecF"
     CDS             complement(7479..8558)
                     /gene="rffT"
                     /locus_tag="ACHY09_000804"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFS3883812.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCLALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMVNDRQREVKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8555..9805)
                     /gene="wzxE"
                     /locus_tag="ACHY09_000805"
     CDS             complement(8555..9805)
                     /gene="wzxE"
                     /locus_tag="ACHY09_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFS3883813.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQELVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9807..10937)
                     /gene="rffA"
                     /locus_tag="ACHY09_000806"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9807..10937)
                     /gene="rffA"
                     /locus_tag="ACHY09_000806"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFS3883814.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKYNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10942..11616)
                     /gene="rffC"
                     /locus_tag="ACHY09_000807"
                     /gene_synonym="wecD"
     CDS             complement(10942..11616)
                     /gene="rffC"
                     /locus_tag="ACHY09_000807"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFS3883815.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPEAPLLTPDALAPWSRV
                     QAKIAASNTDELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRSASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11594..12475)
                     /gene="rfbA"
                     /locus_tag="ACHY09_000808"
     CDS             complement(11594..12475)
                     /gene="rfbA"
                     /locus_tag="ACHY09_000808"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS3883816.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12494..13561)
                     /gene="rffG"
                     /locus_tag="ACHY09_000809"
     CDS             complement(12494..13561)
                     /gene="rffG"
                     /locus_tag="ACHY09_000809"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS3883817.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SVSKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13561..14823)
                     /gene="wecC"
                     /locus_tag="ACHY09_000810"
     CDS             complement(13561..14823)
                     /gene="wecC"
                     /locus_tag="ACHY09_000810"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFS3883818.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14820..15950)
                     /gene="wecB"
                     /locus_tag="ACHY09_000811"
     CDS             complement(14820..15950)
                     /gene="wecB"
                     /locus_tag="ACHY09_000811"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFS3883819.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16006..17052)
                     /gene="wzzE"
                     /locus_tag="ACHY09_000812"
     CDS             complement(16006..17052)
                     /gene="wzzE"
                     /locus_tag="ACHY09_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFS3883820.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYFRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17064..18167)
                     /gene="wecA"
                     /locus_tag="ACHY09_000813"
     CDS             complement(17064..18167)
                     /gene="wecA"
                     /locus_tag="ACHY09_000813"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFS3883821.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18407..19666)
                     /gene="rho"
                     /locus_tag="ACHY09_000814"
     CDS             complement(18407..19666)
                     /gene="rho"
                     /locus_tag="ACHY09_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS3883822.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19751..19852)
                     /gene="rhoL"
                     /locus_tag="ACHY09_000815"
     CDS             complement(19751..19852)
                     /gene="rhoL"
                     /locus_tag="ACHY09_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFS3883823.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19993..20322)
                     /gene="trxA"
                     /locus_tag="ACHY09_000816"
     CDS             complement(19993..20322)
                     /gene="trxA"
                     /locus_tag="ACHY09_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFS3883824.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20453..21718
                     /gene="rhlB"
                     /locus_tag="ACHY09_000817"
     CDS             20453..21718
                     /gene="rhlB"
                     /locus_tag="ACHY09_000817"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFS3883825.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAQATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21852..23336
                     /gene="gppA"
                     /locus_tag="ACHY09_000818"
     CDS             21852..23336
                     /gene="gppA"
                     /locus_tag="ACHY09_000818"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFS3883826.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFYDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23383..25404)
                     /gene="rep"
                     /locus_tag="ACHY09_000819"
     CDS             complement(23383..25404)
                     /gene="rep"
                     /locus_tag="ACHY09_000819"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFS3883827.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25491..25772
                     /gene="ppiC"
                     /locus_tag="ACHY09_000820"
     CDS             25491..25772
                     /gene="ppiC"
                     /locus_tag="ACHY09_000820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFS3883828.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25818..27293)
                     /gene="ilvC"
                     /locus_tag="ACHY09_000821"
     CDS             complement(25818..27293)
                     /gene="ilvC"
                     /locus_tag="ACHY09_000821"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFS3883829.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETVTEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27443..28333
                     /gene="ilvY"
                     /locus_tag="ACHY09_000822"
     CDS             27443..28333
                     /gene="ilvY"
                     /locus_tag="ACHY09_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFS3883830.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLSTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28330..29874)
                     /gene="ilvA"
                     /locus_tag="ACHY09_000823"
     CDS             complement(28330..29874)
                     /gene="ilvA"
                     /locus_tag="ACHY09_000823"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFS3883831.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29877..31727)
                     /gene="ilvD"
                     /locus_tag="ACHY09_000824"
     CDS             complement(29877..31727)
                     /gene="ilvD"
                     /locus_tag="ACHY09_000824"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFS3883832.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31792..32721)
                     /gene="ilvE"
                     /locus_tag="ACHY09_000825"
     CDS             complement(31792..32721)
                     /gene="ilvE"
                     /locus_tag="ACHY09_000825"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFS3883833.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32741..33004)
                     /gene="ilvM"
                     /locus_tag="ACHY09_000826"
     CDS             complement(32741..33004)
                     /gene="ilvM"
                     /locus_tag="ACHY09_000826"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFS3883834.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33001..34647)
                     /gene="ilvG"
                     /locus_tag="ACHY09_000827"
     CDS             complement(33001..34647)
                     /gene="ilvG"
                     /locus_tag="ACHY09_000827"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFS3883835.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34650..34700)
                     /gene="ilvX"
                     /locus_tag="ACHY09_000828"
     CDS             complement(34650..34700)
                     /gene="ilvX"
                     /locus_tag="ACHY09_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFS3883836.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34787..34885)
                     /gene="ilvL"
                     /locus_tag="ACHY09_000829"
     CDS             complement(34787..34885)
                     /gene="ilvL"
                     /locus_tag="ACHY09_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFS3883837.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35238..36758
                     /gene="yifB"
                     /locus_tag="ACHY09_000830"
     CDS             35238..36758
                     /gene="yifB"
                     /locus_tag="ACHY09_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFS3883838.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVQGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36784..37122)
                     /gene="maoP"
                     /locus_tag="ACHY09_000831"
     CDS             complement(36784..37122)
                     /gene="maoP"
                     /locus_tag="ACHY09_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFS3883839.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37241..38080
                     /gene="hdfR"
                     /locus_tag="ACHY09_000832"
     CDS             37241..38080
                     /gene="hdfR"
                     /locus_tag="ACHY09_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFS3883840.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38175..38250)
                     /locus_tag="ACHY09_000833"
     tRNA            complement(38175..38250)
                     /locus_tag="ACHY09_000833"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38215..38217),aa:Trp,seq:cca)
     gene            complement(38259..38335)
                     /locus_tag="ACHY09_000834"
     tRNA            complement(38259..38335)
                     /locus_tag="ACHY09_000834"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38299..38301),aa:Asp,seq:gtc)
BASE COUNT         9392 a        10484 c         9698 g         8800 t
ORIGIN      
        1 tgtcagggag agggatctca attatcaatg cttaattacg tcatcatttt gacgacatga
       61 aaccggagca ttttgcatgg aattttcgtt ttcacccaaa cttcttgttg tggctgtcgc
      121 cgccgctctt ccactcatgg ccaacgcagc agataccccg tcaaccgcca ccgcacgcaa
      181 aggctttgcc ggatacgatc acccaaacca gtatctggtt aaaccggcga ccactattgc
      241 cgacaacatg atgccggtga tgcagcatcc tgcacaagac aaagaaaccc agcagaagct
      301 ggcagaactt gagaaaaaaa ccggtaagaa accgaacgtg gttgttttct tgctggatga
      361 tgtgggctgg atggatgttg gctttaacgg cggcggcgtg gcggtgggta accctacacc
      421 agatatcgac gccgttgcca gccaggggct gattttaact tcggcgtatt ctcaaccgag
      481 ttcttcccca acccgcgcca cgattcttac cggacaatac tccatccacc acggcattct
      541 gatgccgcca atgtacgggc aaccgggcgg tctgcaaggg ttaaccacgc tgccgcagtt
      601 gctgcacgat cagggctacg tcactcaggc catcgggaaa tggcatatgg gggaaaacaa
      661 agagtcgcag ccgcagaacg ttggctttga tgatttccgc ggctttaact cggtatccga
      721 tatgtatacc gaatggcgtg atgttcacgt caacccggaa gtcgccctga gcccggatcg
      781 ttctgaatac atccaaaaac tgccgttcag caaagatgac gttcatgccg tgcgcggcgg
      841 tgaacaggaa gccattgccg acattacgcc gaaatatatg gaagacctgg atcaacgctg
      901 gatggaatat ggcgttaagt tcctcgacaa gatggcgaag agcgataagc ctttcttcct
      961 ctactacggc actcgcggct gtcacttcga taactaccca aatgcgaaat atgcgggtag
     1021 ctctccggcg cgcacctctt acggcgactg catggtggag atgaacgata tcttcgccaa
     1081 tctgtataaa gcactggaga aaaacggtca gcttgataac acgctgattg tgttcacctc
     1141 tgataacggt ccggaagccg aagttccgcc gcacggtcgc acaccgttcc gcggtgcgaa
     1201 aggttctaca tgggaaggcg gcgttcgtgt gccaaccttc gtttactgga aaggcatgat
     1261 ccaaccgcgt aaatctgacg gcattgtcga tctggcggat ctcttcccta cggcgctgga
     1321 tctggcgggg catcctgggg cgaaagtagc gaatttagta ccgaaaacca cctttatcga
     1381 tggtgtggac cagacatctt tcttcctggg aacaaatggt cagtctaacc gtaaggccga
     1441 gcactacttc ctcaacggta aactttctgc tgtgcgtatg gatgagttca agtatcacgt
     1501 cctgattcag caaccttacg cttataccca gagcggatat cagggtggat tcaccggcac
     1561 agtaatgcaa acggcgggat cgtcggtgtt taacctgtac accgatccgc aggaaagcga
     1621 ctctatcggc gtgcgccata ttccgatggg tgtaccgcta cagaccgaaa tgcacgcgta
     1681 tatggagatc ctgaaaaaat atccaccacg cgcgcagatt aaatctgact aagccggcgc
     1741 tatcgctgag atctgccttt gccggatgcg atgctgacgc atcttatcca gcctacagaa
     1801 cgctgcaatt tattgaattt gcaatgattg taggccggat aaggcgttca cgccgcatcc
     1861 ggcaatcaac cgcaggcggc cgccgatttc tacttattca ccaccatcaa atgcgcctgc
     1921 ataatgtcgc tggccggacg accgtgcgcc agcaaatcag ccattgcttt aagatatggc
     1981 ggtagatggc ggaaataacg ctgatacccg gcacacaaat aattcagccc gggtttgccg
     2041 ctggcatcga gcataaagcg gtgtttcggg cagcctcccc agcacgcttt taacacgttg
     2101 caactgcgac actgtgccgg taactgcttg aatttatctt caccaaatgc ctgctgttgc
     2161 ggggaatcaa tcatttctgc aatcgtctgc tgaagcatat tccctagtcg atactgcgga
     2221 taaacatagt gatcgcaggc gtaaacatcg ccgttgtgtt caacaatcac cgagcgccca
     2281 caggttggct gatgatggca aaccgcgccc ggtgcgccga caaaattggc gaacgcccat
     2341 tcgatattca ttacgaaaac cttgccgacg tcgcgcttaa tccagtggtc gaaaatcgcc
     2401 accagaaact caccgaactc ttgcgggcac accgaccatt ccgtcagttc gccctgaata
     2461 tcgccgggag catgtagctt aagtccagca tgagcagccg tttcatcagc caggcgctcg
     2521 accaccggaa taaactggat gaactcaaca cctgcatcac acagaaaatc atagacttgc
     2581 aaaggttgta acgcactggt gcgatttacg cagaccagca cgttatagtc gacatgatgt
     2641 ttttgcagga gcgtcagggc acgcatcacc agcttatgcg ttggtctgcc gcctttggtc
     2701 acgcgatatt gattgtggat ctcagccgga ccatccagcg ataacccaac aagaaaatga
     2761 ttttctgcca gaaatgcaca ccattcgtca tcaagcagca cgccgttagt ctggaagctg
     2821 ttacttatct tcctgccagc accatatttc gcctgtagcg ccacggcacg gcggtaaaaa
     2881 tccagcccga gtaacgttgg ttcgccgccc tgccaggtaa aagccacttc gttttgggtt
     2941 tcgctggcag cgatatagtg acggacatac gcttccagcg tgtcatcgtc catatgcgtg
     3001 actggctttt cgcggtaaag ggattgtttt tcgagataaa aacagtagtc acagttcaga
     3061 ttacaatcgg aaccgctcgg tttcgccatc acatgaaaag cacgcgttgg aacctgttgc
     3121 agcatggtcg ctccttgcct gaagtcatcc ttcaataagt taagggcgaa atcataccgt
     3181 ggcgcaatgc gttattacgg gcgtttattg cgaagggaaa gtgacgaaat gcacagaaaa
     3241 caaaaaaccc cgccgaagcg gggttcaaaa ttggtcggcg agagaggatt cgaacctccg
     3301 acccactggt cccaaaccag ttgcgctacc aagctgcgct actcgccgaa atactgcttt
     3361 ttgaattttt agttcaattc tttaaagtcg tggtgcgagg ggggggactt gaacccccac
     3421 gtccgtaagg acactaacac ctgaagctag cgcgtctacc aattccgcca ccttcgcatt
     3481 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
     3541 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
     3601 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
     3661 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
     3721 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgcg
     3781 cgtttggtta cggtttgtgc gctttgctgc ttatttctcc agtttatgcg ctttaccgtg
     3841 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgtc agcataaaga tgatgccgac
     3901 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
     3961 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
     4021 tgccgcttta tgcgcacgac gaaaacgcag ctggctaatc aatatcacaa accatggcac
     4081 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
     4141 gatgtagttc aggcatgagc caattaacag gatagcaata gatactgcca cacccgcaac
     4201 cggtacgccg tgacgggaaa ctttcgccat cgccgtcggt aactgacggt ttttcgccaa
     4261 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
     4321 cagcaccaca aagttgataa tgcccgccgc tgcggtaatg ccgattttgg cgaaagtcag
     4381 tacgaacggg ctgccgttac tgcctatttc attccacggg aagatggtga cgataacgaa
     4441 aatcgcgcct acgtagaaaa tcaggatacg ccacaacact ttgcctacgg cactgcgcag
     4501 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     4561 ggacgccacc acaatacaca atgcggtcag gaaccctttc cagccacccg caaagaaacc
     4621 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa aaccaaagaa
     4681 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     4741 ccagaactcg atttcgccgt acaaccgcac tgccgccaga ttcgccagcg ccaccagcgc
     4801 caccgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact ggacataaac
     4861 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     4921 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     4981 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga cgaacagccc
     5041 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     5101 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     5161 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     5221 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     5281 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     5341 acaggcgacg gagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     5401 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     5461 aaagcaggcg gtagagccac tccagcccca gcgtttgcca gattttcggt gcgcgtttta
     5521 cgtgaccggt gaaaacatcg taggtcccgc caacgcccat atacagcgca tctggatgca
     5581 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     5641 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     5701 aaccatcctg actgccaacg atattcacat tccactggtt gcgcagttta gcttcagttt
     5761 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     5821 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     5881 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     5941 taattagctc cctgacctca gcattatctt caatggtcag cattttttcg gcattaatgg
     6001 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcgtgctgca
     6061 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     6121 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     6181 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     6241 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     6301 catattgaag atcgccccga agcagaaact gtgcaatatc gcagccttat agcgattagt
     6361 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagac caaccacaat
     6421 cgccccgagc gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     6481 cgagatcgcc agcccggagt ggttattcag cacttcccag gtaaagtagt tggcggagtt
     6541 cagtaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     6601 gacaatggga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     6661 attctcccac ggcgaaaagg tatcgcgggt gagatagaga aacgtataga acgcttcatc
     6721 gccgctcaca ttcattccat aacgttttag cgccagccag aacatgccca caatccccag
     6781 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     6841 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tgtaagtcaa
     6901 caagccaaag gcaaccgtgc tgacgaggaa aaacagccac gctttgctat cctggcgcag
     6961 aaaataaacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta atgccacgcc
     7021 ggagacttcg ctggaaaaga tctgactgta agaattaagc cggaacagca aaaagccgtt
     7081 gtgcatgaag aagatgccga cgctcaccag agcgatgccc atcaggatca cccacgtgag
     7141 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtgcatcag caacgcgttt
     7201 gcgtagacgg gttttgtagg tgacatagta aaccgcgtag aagcagcctg cagaaagcaa
     7261 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     7321 ggtcagcgga aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     7381 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca acgtgcagag
     7441 cagccagaca acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     7501 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     7561 ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcatcg
     7621 gtagtaaaca gtactggcaa atgctgttcc gtcatatcct gccagaacgg attttcccgg
     7681 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     7741 cgggcaaaaa taaagtaacc gagatcgcac tgacgaagta gcgtcagata ggcgtcaaat
     7801 tccagttttt cgctcagaac ttgcagattt tcttcgctga ataactccag tcccgcctga
     7861 cgaacttcct cgatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     7921 accgtatcgc caaattgctg atgaacggcg cgcaaggcag caatatgctc attgctgcgg
     7981 tcaccggagt tccccaccag aatggtcatt tttacttcac gttgccgatc gttcaccatc
     8041 gtattaagag aagagtccat ccgcgtcggg aagtacagca gttcgccccg cacctttggg
     8101 tgcgttttgg caaaaaagct caaatcaccg cgggtggcaa atacacagcc gactcgcttt
     8161 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagttcgtac
     8221 aggtctgccc cccagatatg ccagtaaaac tggctgggct taatgccacc actcagcaga
     8281 gccagccaca gtgtgggatt gaactgaccg tggaagaaaa aacgctgctg acggttagct
     8341 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     8401 agcgccagac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     8461 tgctcgctcg tcgcggccag cgcgtcgttg aaaaaccgca gaacggttcg gttatggtga
     8521 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     8581 acacgccaca acaaagagaa aaatagacga tataggttgc catataggcc tgcgctgcgc
     8641 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     8701 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     8761 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     8821 cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcaac cagacggtaa
     8881 aactcgccgc cgccactgcc ggtaagacga attttagcga tttaaccact tcccgggtga
     8941 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     9001 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacctcat
     9061 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     9121 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     9181 agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg atggcggcag
     9241 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     9301 aacggtaact gacgtagtac gcaagaacgc caatcaagct gccaacaatc agcgataacg
     9361 cattacctgc ggcatcgcga aagcctttca tcaacgccag taacaggttg ccccaggcga
     9421 tccccatttg caccaacgcc actaaacgca ccagctcctg atagtcggta ttaccaaaca
     9481 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     9541 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgggga ttatcatggt
     9601 actgggcaac gtatttggtt acgccgttaa agatgcctgc cccggcaagc acgccgagca
     9661 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacactg
     9721 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgcagtcc
     9781 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaggt cgcaattacc
     9841 gtacgctgat tgacgggcga caggttgtag aacaacggca ggcgcagcag gcgctcgctc
     9901 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc cgcagggcaa
     9961 ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag aaagttaatc
    10021 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
    10081 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg tgctaacgca
    10141 tcgtagtagt tttgccacag cgccagacgt tgctgattga tacgatccgc tgcttccagt
    10201 tgcgcccaca ggtacgcagc ttgcaaatcg gacatcaaat agctggagcc gatatcgcgc
    10261 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc tttttcacgg
    10321 atgatctcgg ctcgttcgat caacgcttta tcgttaatca gcgtcgcacc gccttcaccg
    10381 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
    10441 agtgcacgcc ctttgtaagt ggacataacg ccctgagcgg catcttccac cacaaacaga
    10501 ttatactttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
    10561 accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt ttcgtcgatg
    10621 ttcatagtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
    10681 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
    10741 agcagcagcg ccgccatctc cagagaagcg gtgcaggacg gcgtcaacag cactttggcg
    10801 ctgccaaaac gttgttccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
    10861 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
    10921 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
    10981 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atctgggtcg ccacccgcaa
    11041 agttgtttta ccgcgagcat acgcccagtt tagcgctgtt tgcatcagct cagcacctgc
    11101 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
    11161 atagccgcga atatcgccgg acgccgaacg taaaatgagg cattgatgat caaaggtgcc
    11221 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
    11281 cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta atgcgggaat
    11341 atcggtctct tgtgccacta cagcaccact atcactgaca ttgtttacgg gtagcgccaa
    11401 atcaacttca ccttctacca gggagaatcc cagctgttgc aaggcatcca gttcatccgt
    11461 atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaaggcgt ctggcgtcag
    11521 gagcggtgct tcaggactaa tgcgcacaat ggcgctgtta acaccaaaga aggcgttttc
    11581 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
    11641 cgtagccagt tttcgccagc gagctggcgg cgcgcttcac gccttcgtca tcaagccagc
    11701 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttccaccg
    11761 tctgcacaaa ggtgctggct tcaatcaggc tgtcgtgagt gccagtgtcc agccaggcaa
    11821 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
    11881 aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac tccacgactt
    11941 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
    12001 cttccagcga gatagcgcgg aaattgtcgt caaactccac gacgccaaag cgttccggat
    12061 ccatcacctg atagccaaat accgtcgcgc cttgcgtgcg cgccgcaaca tgacgcagct
    12121 tcggactgaa gccctgtccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
    12181 cgttgaggaa ggtttcaccg atgataaagg cctgcgccag accgtccgga ctgggctgtt
    12241 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
    12301 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
    12361 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
    12421 gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct ttcatgtttg
    12481 cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca
    12541 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
    12601 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
    12661 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtgcgcca
    12721 cgccgtgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt tccacaacat
    12781 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
    12841 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
    12901 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
    12961 tcagtttttc cggaaagtgg taagggccgt agttattcga gcagttggtg ataagcgtcg
    13021 gcagaccata ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacacgg
    13081 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
    13141 ggtcaccata tacttcgtcg gtagagatat gatgaaaacg gaacgctgat tttttatctt
    13201 ccgtcagcgc attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
    13261 tggtttcaac aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
    13321 gatgcatgac acagtctggc tgatgctcag tgaatacgcg cgccagttct gcccgatcgc
    13381 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
    13441 gatttccggc gtaggtcagc ttatcgacca ctaccaccgc gtcgctcgtt tcgttgatga
    13501 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    13561 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    13621 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    13681 gtcaagctgc gccagtgtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    13741 ctcaacaacc agagtttcgc cgctatgcca ctgggcgatc agttcagcga tttccatcgc
    13801 tgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    13861 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    13921 atcgataacc cagaacggtt tgtgatcgtt cacttcgcgc gcggtacgga taagccgcgc
    13981 ctgctgcggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    14041 gccaggacca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    14101 ccagacgtta attccctgat cggcacaaat cagcgacagt tcgttagcaa aagcgatatt
    14161 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    14221 gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggccg aacaaaccgg
    14281 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    14341 cacgcgttcc gggcagtaag caatgttgac gtccgccaac tcgcccacct gctgtgggaa
    14401 agtgagatct ggacgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    14461 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    14521 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg tgggtacagc
    14581 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    14641 ggcagttttt actacgctcg ccaaatcagg ttcgacgata tggatttcgc cacgattgat
    14701 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    14761 cgcagctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    14821 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    14881 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    14941 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    15001 accgcttccg gacgctcagt ggtatcgcgc attaccagca ccggtttccc cagcgaaggc
    15061 gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt catcagccag
    15121 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacgtg ccccagaatg
    15181 cgattgaccg gctctctgac gttcgggttg agatgcactg gatagacaat ctggatgtcc
    15241 tggtgcgtgg tggcgatgtc tgccagcgca tggcagattt cttcaaagcc acgaccgaag
    15301 ctctcacgcc tgtgaccggt caccagaatc atttttttat tggggtcgat aaacgggtaa
    15361 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    15421 agtgcatcaa tgactgtatt accagtaatg aagattcggc tatccgcaac gttttcacgc
    15481 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    15541 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg cagaccagcc
    15601 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    15661 gtagtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    15721 ccttccagaa tccgacaggt tatctctgta aggccctgtc ctggctgcat tatgttgaga
    15781 tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc ccgatgctgc
    15841 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacggc
    15901 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    15961 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    16021 gcgggttaat gcgacaccag ccccgatcag tcccccgaca atgccccaca taatcatcag
    16081 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgaa aatagcgata
    16141 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggcccgatt
    16201 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    16261 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    16321 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgtt caatactgtt
    16381 catccggcga tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    16441 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    16501 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cagtttcggc
    16561 aatcagcttc acgctgtcat tgaccgcgcg ggtgaagtca ccgggaataa acacgatgtt
    16621 gttgatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    16681 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    16741 aaactcttta taagcttcgt ccatgaccga tggttggtcg gcagaagcca tgtttgaacg
    16801 gacatccagg ttacgcaaaa attgctgctg tgagtaatat ccccccagca tattcaccgt
    16861 tggacgatcg gtaatcgctg tcgagctcca ctcctggcga gcaaaaaaag tatacgccag
    16921 cgcgattaac gcaaacgcca gccccatgcc aataatccac agcttcccag cccacaaggt
    16981 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    17041 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    17101 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttgatgcaa
    17161 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    17221 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    17281 acaaacgcct ggcgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    17341 tctggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    17401 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg agttttgccc
    17461 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatcc
    17521 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    17581 atggcggcga tcatcgcaaa gcaccagatt gcgagactgg tttgcccgtc gaaccacaaa
    17641 atcataccga ttgctgcaaa cgagacgcag gacaacccac ccagcaagcc atcaatgcca
    17701 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    17761 aacggtccga gcaccatctc ccaggagcca aagatataac ctaggctact gagataaagt
    17821 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    17881 ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccagc acaagcgaga
    17941 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    18001 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagttaggt
    18061 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    18121 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    18181 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    18241 tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg cataacacac
    18301 cagcaaaatc caggaatttt cctaacattg ccagcgcggc attaagatta cagatataaa
    18361 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    18421 tcatttcgaa gaaatcgtca ttggtcttgg tcattgccag tttattaatg aggaattcca
    18481 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    18541 cttcctgagt agtgagcagc tcttctttac gggtaccgga acggttgtag tcgattgccg
    18601 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    18661 taaactcttc gtagataact tcgtccatct tggaaccggt atcgataagc gcggtagcga
    18721 tgatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag cgtttcggac
    18781 gatgcagggc gttggcatcc acaccaccgg tcagcacttt acctgacgcc ggaacaacgg
    18841 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag gatgataacg tctttcttgt
    18901 gctcaacaag gcgtttcgcc ttctcgatca ccatttccgc gacctgaacg tggcgagatg
    18961 ctggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    19021 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    19081 caatgctctg agcaatgttc tgcagcagca tggttttacc ggctttcggc ggtgccacaa
    19141 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcggttaaat
    19201 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    19261 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    19321 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    19381 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    19441 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    19501 ccagtacacc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    19561 aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt tcgccgagag
    19621 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    19681 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    19741 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    19801 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    19861 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    19921 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    19981 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    20041 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcaaag tcgggatacc
    20101 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    20161 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    20221 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    20281 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    20341 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    20401 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    20461 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    20521 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    20581 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    20641 tcttacgtca acgtttcatt atcttctctc tcatcccgcg attgccgatc gcaaggtgaa
    20701 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    20761 cgcggagcca cttgcgcaag ctaccggcct gaagctgggt ctggcttacg gtggtgatgg
    20821 ctacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    20881 gcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    20941 actggacgaa gccgatcgta tgtacgatct gggctttatt aaagatatcc gctggctgtt
    21001 ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta
    21061 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    21121 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    21181 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    21241 caacaccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    21301 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    21361 ccgtggtgat ttggatattc tggttgccac cgatgttgcc gcgcgtggcc tgcatatccc
    21421 ggctgtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    21481 tattggtcgt acaggtcgcg ctggcgcaag tggtcattcc atcagcctgg catgtgaaga
    21541 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    21601 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    21661 tacaggcaat ggtccgcgtc gtactggtgc tccgcgtaat cgtcgtcgtt caggttaaat
    21721 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    21781 gcgcatgctg tagtaagtga tttgggtgaa agaacgcagc caacacagag acagattgaa
    21841 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    21901 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    21961 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgccctgtct aatgaagcaa
    22021 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    22081 cgcaaattcg cgttgtcgct acggcgacat tacgactagc cgtcaatgcg ggtgatttta
    22141 tcgccaaagc acaggaaatc cttggctgcc cggtacaagt catcagcggt gaagaagaag
    22201 cccgcctgat ttatcagggg gtggcccaca caacaggcgg tgccgatcag cgcctggtgg
    22261 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    22321 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    22381 tggcgcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    22441 cagatgaatt acggtatcac ggctggaaag tgtgcgttgg cgcttccggc accgtgcagg
    22501 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    22561 agcaactgaa acaacgcgcc attcattgcg gtcggctgga agagctagag attgacgggc
    22621 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgata gccattttta
    22681 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    22741 acggtatgtt gcatcttacc gtcgagcagg atattcgcag ccgtacgctg cgtaatattc
    22801 agcgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    22861 tctacgatca ggtggaaaat gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    22921 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagcg ccgcaacacg
    22981 ctgcttatct ggtacgtaat ctggatcttc ccggttttac ccccgcacag aaaaaactgc
    23041 tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg catcagcaaa
    23101 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    23161 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    23221 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    23281 ttgatcagga aagccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    23341 taagtgccgg atgcgatgct gacacatctt ttccggcctt gattatttcc ctcgttttgc
    23401 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    23461 gctgaccact ttgcgctcct gttcccaaat caaatcatcc tgcggcagct ccagcaggaa
    23521 gcggctcggc tccgggcgca ccagttctcc gtactgacgg cgttctttac acagcgtaaa
    23581 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    23641 attatcttca tcgatgctgc tctggtgcgg caaaaacccc tcttccatac cgaccatgta
    23701 gacataagga aactccagcc ctttcgacgc gtggagagtc atcagttgca cctgatccag
    23761 ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg tcaccacctg
    23821 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    23881 cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg atgtttcgta
    23941 cagccaggat tcataatcca tgccgtggat cagatcacgc accgcggcaa tcggctcccg
    24001 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    24061 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    24121 gcgcgtcatc gcccattcac ccaacttttt cagcgtcgcc gggccaatct ctcgcttcgg
    24181 cgtgttaacg atacgcagga acgcactatc atcgtccggg ttagtcagca cgcgcagata
    24241 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    24301 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatggt taccgcgata
    24361 aagaatggcg taatctttgt actgcgtttt attgacgaag tgatgggcaa tcagctcgcc
    24421 agtaacgcgc tcggcttcat gttcctcgtt attcgcgctt aaaactttta gctctgtgcc
    24481 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    24541 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    24601 cagcgccgga aaatcctgac tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    24661 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    24721 tttcaccagc tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    24781 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcaacagca acgttggcaa
    24841 taaaatcaga tcatcgaaat cgagaacgtt acaggctttc aggtgtgcat catacagccc
    24901 ataacaatgg gcaaaaatac ggtcccgctc gccctttgcc tctgccgccg cctgcgccgg
    24961 tgttttgaga tcgttcttcc agttggagat ggtcgaaatt agctgttgca ggagaacttt
    25021 gtcatcttca atcagcccct cggtcaactc tttaagcaaa gcaagctgat cggtatcgtc
    25081 aaacagcgaa aagttagcct tcatccccaa cgccgcgtac tcacgcttaa tgatatccag
    25141 ccccagcgtg tgaaaggtgg agatcatcag cccacgcgcc tctttacgcc ccagcgtctg
    25201 cccgacacgc tctttcatct cgcgtgccgc cttattggta aaggtcaccg ccgcgatatg
    25261 tcgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga taacacgagt
    25321 tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    25381 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    25441 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    25501 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgagc
    25561 agatcaagaa tggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccgtcag
    25621 gcaaacgcgg cggtgatttg ggtgaattcc gccagggtca aatggttccg gcgttcgata
    25681 aagtggtttt ctcttgtccg gtactggagc caaccggccc gctgcatacc cagttcggtt
    25741 accacatcat taaggtgctg taccgtaact aacagcaagg ccttctccgg gagaaggcct
    25801 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    25861 cgcagtttct tacctacctg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    25921 agctgcgcgt tatctaccgc accttccgga atagctttac ccaggtcgcc cggttgcagc
    25981 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    26041 tcggcagtat cggagataac cacgttcatt tcgtacagac gcttacgggc gatggtgttg
    26101 gcaatcagcg gcaattcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    26161 tcaaccatgg tttcaaacgc caactcaacg cctgctttta ccatcgcgat catcagtacg
    26221 cctttatcga agtactcctg ttcgccgatt ttgccttcat actgcggcgc ggtttcgaat
    26281 gcggttttgc cagtctcttc acgccaggtc agcagtttct tatcgtcgtt ggcccagtca
    26341 gccatcatgc cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    26401 ggcgccatga tctctttcag ttgctcagag agcgcgtaag cacgcagttt cgccgggtta
    26461 gaaagacggt ccatcatcag ggtgatgccg ccctgtttca gcgcttcggt gacggtttcc
    26521 caaccgaact gaatcagttt ttctgcgtat gccggatcgg tgccttcttc caccagcttg
    26581 tcgaagcaca gcagagaacc agcttgcaac ataccgcaca ggatggtttg ctcgcccatc
    26641 aggtcagatt tcacttccgc aacgaaagag gattccagca cgcccgcacg gtgaccgccg
    26701 gttgcagccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    26761 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg tacttcggtg
    26821 ccagggcatt tcggcgcaac cattacgacg gtgatgtctt tacggatctg ctcgcccact
    26881 tcgacgatgt tgaaaccgtg tgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    26941 acggtgcgta ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    27001 tgcgggatca gttcttcgta agtacccact ttaaaaccat tttcggttgc tttacgccag
    27061 gatgcgcgct tctcggcaat cgcttcttta cgcagagcgt aagaaatatc cagaccagaa
    27121 tcacgcatgt tcaggccctg gttcagcccc tgtgcgccac agccgacgat gactactttt
    27181 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    27241 cccagctgtg ccagctgctg gcgcagattc agtgtattga agtagttagc catggtgatt
    27301 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    27361 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    27421 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    27481 ggaaagccgc cattttggcc gtagcgcgcg ggcaatgcac gttagcccgt ctacgctttc
    27541 gcgacaaata cagcgcctgg aagaagatct cggtcagccg ctgtttgtgc gtgataaccg
    27601 cacggtaacg ctgacggaag cgggcgaaga gctgcgcgtt ttcgcccagc aaacgctgtt
    27661 gcagtatcag caattgcgcc acaccatcga ccagcaaggg ccgtcgctct ctggcgaatt
    27721 acatatcttc tgctcggtga ccgctgccta cagccatctg ccgccgattc tggatcgctt
    27781 ccgcgcggaa catccgtcag tggagatcaa actctctacc ggtgatgcgg ccgatgcgat
    27841 ggaaaaggtg gtcactggcg aagcggatct ggcgattgcc gggaaaccgg aaaccttgcc
    27901 gggcgcagtg gcgttttcga tgctggagaa tctggcggtg gtgctgattg ctcccgcgct
    27961 gccctgcccg gtgcgtaatc aggtatctgt cgaaaagccc gactggtcta cagtgccgtt
    28021 tatcatggcc gatcagggac cggtacgtcg ccgcattgaa ctgtggtttc ggcgtaacaa
    28081 aatcagcaac ccaatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    28141 ggcgctcggc tgtggcgtgg cgctgttgcc ggaagtggtg ctggataaca gcccggaacc
    28201 ggtgcgtaac cgcgtgatga ttttagagcg cagtgatgaa aaaacgccgt ttgagctggg
    28261 cgtgtgcgta caaaaaaagc ggcttcatga gccgctgatt gaggcgttct ggaagatttt
    28321 gccgaaccac taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    28381 atcgtagccc agttcattca gccgggtttc aaaatccggt tcatgatcgc caagctcgaa
    28441 cgccgccagt acgcgcccgt agtcggtacc gtggctgcga taatggaaca gcgagatgtt
    28501 ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggcgatt ccgggaattc
    28561 gaagctgtag aggcgttcct gcaacggatg cgatggacgc ccgccaacca tatagcgcac
    28621 atgcagcttc gccatttcgt cgtcggagag atccaccacg ctgtagccgc catcgttgag
    28681 catctgcaaa atttctttgc gctcttcgag gccacggctc aggcgcacgc cgacaaaaat
    28741 gcaggcgttt ttggcatcag caaagcggta gttgaactcg gtgaccgagc gcccaccaag
    28801 taactggcag aatttgagga agctgccctt ttcttccgga atggtcaccg ccagtaacgc
    28861 ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag cgcagaccgt gaaagttcac
    28921 gttggcaccg gaaagaatat gcgccagccg ctcaccgcga atgttgtgct gggcgatgta
    28981 ttttttcatc cctgccagcg ccagcgcgcc agagggttca gccaccgcgc gtacatcttc
    29041 gaacaggtct ttcatcgccg cacagatggc atcgctatcg acggtgatga tgtcgtcaag
    29101 atactcctgg cacaaacgga aggtttcatc gccgatgcgt tttaccgcga cgccttcagc
    29161 aaacagcccc acgcggggca gatcaacggg atgacctgca tcaagtgccg ctttcaggca
    29221 ggcggaatct tccgcttcta cggcgatcac tttgatttgt ggcatcagtt gtttgatcag
    29281 caccgccacg cccgccgcca gaccgccgcc gccgaccgga acaaatacgc ggtcgagatg
    29341 agcatcctgc tggagcagtt ccagcgccag cgtaccctga ccagcgatca ccatcggatg
    29401 atcgaagggt ggcacccagg tgaagccctg ctgctgtgac agttcgattg ctttcgcttt
    29461 cgcttcgtca aagttcgcgc catgaagcag cacttcgccg ccaaagccgc gcaccgcatc
    29521 aactttgata tcggcggtgg cggttggcat gacgatcagc gccttcacgc ctaaccgtgc
    29581 ggaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcggtaatca cgccgtgcgc
    29641 tttttgttct tccgtcaggc ccgccatcat ggcgtatgca ccacgaagct taaagctgtg
    29701 cactggctgg cgatcttcgc gcttcaccag aatcacgtta tcaagacgcg acgacagttt
    29761 ttccattttc tgtagcggcg tgacctgcgc cgcttcgtag accggtgcgc gcagcaccgc
    29821 tcttaaatat tcggcacctt ccggggcacc ggacaggggt tgtgagtctg ccatcattaa
    29881 ccccccagtt tcgatttatc gcgcaccgcg cctttgtcgg cgctggttgc caggctggcg
    29941 taggcgcgca gggcaaagga gacctgacgt tcacggtttt tcggcgtcca ggctttgtca
    30001 ccccgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    30061 ataccacggt tcggaatgtc gatggcgatc aggtcgccgt cttcaatcag gccaatgctg
    30121 ccgccgcttg ccgcttccgg tgagacgtgg ccgatagaaa ggccagaggt accgccagag
    30181 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg
    30241 gttgggtaga gcatttcctg catccccgga ccgcctttcg ggccttcata gcgaattact
    30301 accacatctc cggcgacaac tttaccgccg agaatcgctt ctaccgcatc gtcctggctt
    30361 tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catccacgcc cgcggtttta
    30421 acgatgcagc cgttttccgc aaagttaccg tagagcaccg ccaggccgcc gtctttgctg
    30481 taggcgtgtt ccagcgagcg gatacagcca ttggcgcgat cgtcgtccag cgtatcccaa
    30541 cggcaatctt gcgagaatgc ctgggtggta cggatacccg cagggccggc gcggaacata
    30601 ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagcgt ttgcggcaac
    30661 gtcaggccga gcacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcgccg
    30721 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ctgggtgctc
    30781 ggcgcaactt tacacagctg tggaaccttg cgggaaagct tatcgatatc actcatggtg
    30841 aagtcgattt ccgcttcctg cgccgccgcc agcagatgaa gtacggtgtt ggtagagccg
    30901 cccatcgcga tatccagcgt catggcgttt tcaaacgccg ccttactggc gatattacgc
    30961 ggcagtgcac tatcgtcgtt ttgctcgtaa taacgtttgg tcaattcaac aatgcgttta
    31021 ccagcattga ggaacagctg cttacggtcc gcgtgggttg ccagcaacga gccgttgccc
    31081 ggctgcgaca ggcccagtgc ttcggtcagg cagttcattg agttagcagt aaacatcccg
    31141 gagcaggaac cgcaggttgg gcaggcggat cgttcaacct gatcgctctg ggagtcagaa
    31201 actttcgggt ctgcaccctg gatcatcgca tcaaccagat cgagcttaat gatctgatcg
    31261 gacagtttgg ttttcccggc ttccatcggg ccgccggaaa caaagatcac cggaatattc
    31321 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    31381 atggcgtcgg cgcagtgagc gttgaccata tactcaacgg aatcagcgat caattcgcga
    31441 gatggcagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg
    31501 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg
    31561 agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    31621 atcggcttac cgaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    31681 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    31741 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    31801 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    31861 ttgctgaatg cgtttggtaa ccgggccgca acggccttca ccaacctgaa taccgtctac
    31921 actgcgcact ggcgtgattt ctgccgcagt accggacata aacacttcat ccgccaggta
    31981 cagtgattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt
    32041 gatgatggcg tcacgggtaa tacccggcag cgcggatgag gtgaacggtg gggtgaacag
    32101 cacaccgtct ttcacttcaa acaggttttc gcctgcgcct tcagagatgt aaccgttcac
    32161 atccagcgcg ataccttcct gataaccgtg gcggcgtgct tcactaccca ccagcaggga
    32221 agagaggtag ttaccaccgg cttttgccgc ggttgggatg gtgtttggtg ctgcgcggtt
    32281 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgcccc
    32341 ccacgggaaa gcggcgataa tcacgtcggt tgagtatccc gctggcgggt ttacgcccat
    32401 gccaacatca ccaacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    32461 gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgcgaaaccg ggaagcgata
    32521 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    32581 tttgtgcgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    32641 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    32701 atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg
    32761 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    32821 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    32881 catcgctggc ggcggccata ttcattgaac agacgtggaa accacgatga cgcaccacgc
    32941 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    33001 tcatgataat ttctccaaca tttctgaatt actggcacca ggcggcacca gcggccagac
    33061 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    33121 gtcgagtgcc gcttcaacct ggtctttatg ggtgatgtgt tggccaggga tgccgaaggc
    33181 gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg
    33241 gaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    33301 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    33361 gccgtcaccg gagatacaga cgacagtatc gttcggtcgc gcgacttgtg cgccaaccgc
    33421 cgccggtaaa ccgaaaccca tggtgcctaa gccgctggag gtgatgaagt tttccgggcg
    33481 agtgtggacg atgtgctggg cagcccacat ctggtgctgc cccacatctg tggtcacgat
    33541 gcaatccgca gatttacgat ccgacagttg ttttaacaac aacggcgcgt agatagcgtc
    33601 accaggatgg tcgtaacgcc aggcatgttc atcacgcagt tgcgcgcagt gttgctgcca
    33661 gtcatcgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccctgtaa
    33721 tgccacatgt gcctgacgca gcttgttcat ttctgccgga tcgatatcca tatggataac
    33781 gctggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcgcc
    33841 cacggcgatc agtagatcgc actcctgcac tgcgaagttc gccgctttgg tgccgtgcat
    33901 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcaacgt
    33961 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccgaaa ctgcctgcgc
    34021 catgcccacg ccaccaccaa cgtacagcat cggtttttgc gcttttgcca gcatctgacg
    34081 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacagtgg tgaaccacgg
    34141 ttccagatcg ccgctggcta actggatatc ttttggaata tcgaccaaaa ccggaccagg
    34201 acgaccagag ctggcgacgt caaacgcttc ggccatgata cgcggtaact cttccagtga
    34261 ttgcaccagg aaactgtgct tggtgcaggc taacgacaac cccagaatat ccacctcctg
    34321 aaatgcgtca gtgccgataa acggtgcgga tacctgaccg gtgatagcaa caacggggat
    34381 ggaatctaac agcgcgtccg caagcccggt tatcaggttg gttgcacctg gaccagacgt
    34441 ggcgatgcaa acgcccgttt tgccggtagc acgggcataa ccgatagccg ccattgctgc
    34501 accttgctcg tgtcggcaca gcaagtgttc cacgccaccg tcatacaatg catcgtaaac
    34561 cggcataatt gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc
    34621 atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag
    34681 aattttgtgc tgttattcat tgtctgctcc tcagttatgt ttttaaggtc aaaaaaaacc
    34741 cccggacctt tcggtgcggg ggtcttagtt cgttaaggct tgatctctaa gcctttcctc
    34801 gtccaagtgc agccccgcac ggtgggataa taatcaccac cacgctaatc acgaccaggc
    34861 taatcactcg tagaagggct gtcattttgt cttttcttgc atcttgttcg aaggaatgcc
    34921 taaagagtta ccatagattt tgcgaatagc acaagatatt ttttggccga tttttatgat
    34981 gtctttaata atttcaagag aaattgttgt tttttaaaaa gaaaatagtg agtgaaaaaa
    35041 attcaatttc agtatgaatg ttgttccctt cctcgtagtt catcctttgc ccgctgcatt
    35101 tttgcgagca tcattccacc gccagaacaa ctcaacttca cttgcacaaa taaacaggaa
    35161 tcagccacta aaattcacga attagcgggc tacagcagcg atatcttatg cacataatgg
    35221 tgactcaagg agggcttatg tcactgtcaa ttgttcatac ccgcgcagcc ctgggagtaa
    35281 atgcgccccc aatcactgtt gaggtacata tcagtaaagg tctaccaggc ttaacgatgg
    35341 tgggcttacc agaaacaacg gtaaaagaag ctcgcgatcg cgtgcgcagc gccattatca
    35401 atagcggata tgaatatccg gcgaaaaaaa tcaccatcaa ccttgctcca gccgatctgc
    35461 caaaagaagg gggacgatat gatttgccta tcgctattgc gttactggcg gcctcagaac
    35521 agcttacagc caataagtta gatgaatatg aattagtcgg agaactggcg cttacaggcg
    35581 ctctgcgtgg cgttcccggc gcaatctcca gtgcaactga agccattaag tcgggcagaa
    35641 aaattatcgt cgcgaaagat aacgaggatg aagtggggct aattaacggt gaaggatgcc
    35701 tgatagccga tcatctgcag gctgtctgtg cgtttctgga gggtaagcac gctctcgaac
    35761 gcccgaaacc aactgatgca gtatcccggg cgctacaaca tgatctcagt gatgttgtcg
    35821 gtcaggagca aggaaagcga ggactggaaa ttaccgccgc tggcgggcac aaccttttac
    35881 tgattgggcc gccgggaaca ggtaaaacaa tgctcgccag ccgtattaat ggcctgttgc
    35941 cagatttaag caatgaagag gcactggaga gcgctgcgat attaagtctg gtaaatgctg
    36001 aatcagtaca aaaacaatgg cggcagcgcc cattccgctc acctcatcac agtgcgtcgt
    36061 taactgcgat ggtgggcggt ggtgcaattc cagggccagg tgaaatttcg ctggcgcata
    36121 acggcgtgct ttttcttgat gagctacctg aatttgaacg gcgtacactg gatgccttgc
    36181 gagagccgat tgaatccggg cagatccatc tttcacgcac acgagcaaaa ataacctatc
    36241 cagcccgttt ccaacttgtt gcggcgatga atcccagccc taccggacat tatcagggaa
    36301 accataaccg ctgcacacca gaacagacat tacgttatct caaccggctc tcggggccct
    36361 ttctcgaccg cttcgatctc tcactagaga tcccattacc gccccccggc attttgagta
    36421 aaacggtagt gcagggagaa aacagcacca ccgttaaaca acgtgtaatg gccgccagag
    36481 agcgccaatt taagcggcag aataaactga acgcctggct ggataatccg gaaatacgcc
    36541 aattctgcaa gcttgagagc gaagatgcgc agtggctgga agaaacgctg atccatctgg
    36601 ggttatcgat tcgtgcctgg cagcggttat tgaaagttgc acgaaccatt gctgatattg
    36661 atcagtctga cattatcaca cgtcagcatt tgcaggaggc agttagctat cgtgcgattg
    36721 accgtttgct catccatctg caaaaactac tgacataaaa aaaagggcat ttcgcccttt
    36781 ttattaatcg tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga
    36841 aagggtgtga aaacgttttg gacgcttgat acgcgtcata tacttggacc acacgcgttc
    36901 tgcttctgtc actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc
    36961 ggtaaccggc tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag
    37021 cagttgtgcc tctttgatgg tgaagtcacc atgacgagag aatccacgtg gataatgttt
    37081 attgtcgaaa tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc
    37141 tttgaccgag ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa
    37201 catttaaatc ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa
    37261 aactttcctg gaagttagcc gaacgcgtca ctttggtcga gcagctgaat cgctctatct
    37321 gacccaatca gccgtgagct ttcgaatcag acaactggaa aatcaactgg gtgtgaacct
    37381 tttcacccgc cacagaaaca atatccgttt aaccgctgct ggtgaaaaac tactgcctta
    37441 tgcagaaacg ctcatgagca cctggcaggc cgcccgtaag gaggtggcgc atacctcacg
    37501 acataacgag ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg
    37561 gctgggacgc ttgtatcaaa atcaggatgt ccatacaggc ttacagttcg aagcgcgaat
    37621 tgcccaacgg cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac
    37681 tactgaagcg cccaaaatgg acgaatttag cagtcagttg ctcgggtatt tcactttagc
    37741 gctttatacc agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggaatg
    37801 ggggccagat tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct
    37861 gacaaccagt tctgctgaac tggcacagca acagattgcg acgcttaatg gttgcacctg
    37921 gctacccgtc agttgggcgc gtaaaaaggg cggcctgcat accgttgtcg atagcacaac
    37981 actttcacgg ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg
    38041 cgatctattg aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat
    38101 gccggtagaa ggatttactt cggagaggtt atttcagata aaaaaaatcc ttagctttcg
    38161 ctaaggatga tttctggcag gggcggagag actcgaactc ccaacacccg gttttggaga
    38221 ccggtgctct accaattgaa ctacgcccct aattagggtg gcggaacgga cgggactcga
    38281 acccgcgacc ccctgcgtga caggcaggta ttctaaccga ctgaactacc gctccaccga
    38341 attcttttac aaccaccggt tttatgaccg gctt
//
LOCUS       DAZUCC010000018        36922 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZUCC010000018 DAZUCC010000000
VERSION     DAZUCC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.018"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1385)
                     /gene="ycjM"
                     /locus_tag="ACHY09_000835"
     CDS             complement(<1..1385)
                     /gene="ycjM"
                     /locus_tag="ACHY09_000835"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFS3883841.1"
                     /translation="MEQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSTFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYIRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     IDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNN"
     gene            complement(1599..1913)
                     /gene="pspE"
                     /locus_tag="ACHY09_000836"
     CDS             complement(1599..1913)
                     /gene="pspE"
                     /locus_tag="ACHY09_000836"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFS3883842.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1988..2209)
                     /gene="pspD"
                     /locus_tag="ACHY09_000837"
     CDS             complement(1988..2209)
                     /gene="pspD"
                     /locus_tag="ACHY09_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFS3883843.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(2218..2577)
                     /gene="pspC"
                     /locus_tag="ACHY09_000838"
     CDS             complement(2218..2577)
                     /gene="pspC"
                     /locus_tag="ACHY09_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFS3883844.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2577..2801)
                     /gene="pspB"
                     /locus_tag="ACHY09_000839"
     CDS             complement(2577..2801)
                     /gene="pspB"
                     /locus_tag="ACHY09_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFS3883845.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2855..3523)
                     /gene="pspA"
                     /locus_tag="ACHY09_000840"
     CDS             complement(2855..3523)
                     /gene="pspA"
                     /locus_tag="ACHY09_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFS3883846.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3690..4667
                     /gene="pspF"
                     /locus_tag="ACHY09_000841"
     CDS             3690..4667
                     /gene="pspF"
                     /locus_tag="ACHY09_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFS3883847.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4787..6052)
                     /gene="puuE"
                     /locus_tag="ACHY09_000842"
     CDS             complement(4787..6052)
                     /gene="puuE"
                     /locus_tag="ACHY09_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFS3883848.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKMFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDEAMKILQDALSD"
     gene            complement(6090..7370)
                     /gene="puuB"
                     /locus_tag="ACHY09_000843"
     CDS             complement(6090..7370)
                     /gene="puuB"
                     /locus_tag="ACHY09_000843"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFS3883849.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVHELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7372..8859)
                     /gene="puuC"
                     /locus_tag="ACHY09_000844"
     CDS             complement(7372..8859)
                     /gene="puuC"
                     /locus_tag="ACHY09_000844"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFS3883850.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(9009..9566)
                     /gene="puuR"
                     /locus_tag="ACHY09_000845"
     CDS             complement(9009..9566)
                     /gene="puuR"
                     /locus_tag="ACHY09_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFS3883851.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9593..10357)
                     /gene="puuD"
                     /locus_tag="ACHY09_000846"
     CDS             complement(9593..10357)
                     /gene="puuD"
                     /locus_tag="ACHY09_000846"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFS3883852.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10569..11987
                     /gene="puuA"
                     /locus_tag="ACHY09_000847"
     CDS             10569..11987
                     /gene="puuA"
                     /locus_tag="ACHY09_000847"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFS3883853.1"
                     /translation="METNIVEVENFVQQSEERRGSSFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            12136..12300
                     /locus_tag="ACHY09_000848"
     CDS             12136..12300
                     /locus_tag="ACHY09_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883854.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12290..13675
                     /gene="puuP"
                     /locus_tag="ACHY09_000849"
     CDS             12290..13675
                     /gene="puuP"
                     /locus_tag="ACHY09_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFS3883855.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            13809..14054
                     /gene="ymjA"
                     /locus_tag="ACHY09_000850"
     CDS             13809..14054
                     /gene="ymjA"
                     /locus_tag="ACHY09_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFS3883856.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14367..16010
                     /gene="sapA"
                     /locus_tag="ACHY09_000851"
     CDS             14367..16010
                     /gene="sapA"
                     /locus_tag="ACHY09_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFS3883857.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            16007..16972
                     /gene="sapB"
                     /locus_tag="ACHY09_000852"
     CDS             16007..16972
                     /gene="sapB"
                     /locus_tag="ACHY09_000852"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFS3883858.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16959..17849
                     /gene="sapC"
                     /locus_tag="ACHY09_000853"
     CDS             16959..17849
                     /gene="sapC"
                     /locus_tag="ACHY09_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFS3883859.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17849..18841
                     /gene="sapD"
                     /locus_tag="ACHY09_000854"
     CDS             17849..18841
                     /gene="sapD"
                     /locus_tag="ACHY09_000854"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFS3883860.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18843..19649
                     /gene="sapF"
                     /locus_tag="ACHY09_000855"
     CDS             18843..19649
                     /gene="sapF"
                     /locus_tag="ACHY09_000855"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFS3883861.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(19698..20861)
                     /locus_tag="ACHY09_000856"
     CDS             complement(19698..20861)
                     /locus_tag="ACHY09_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFS3883862.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFIRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(20870..22243)
                     /locus_tag="ACHY09_000857"
     CDS             complement(20870..22243)
                     /locus_tag="ACHY09_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFS3883863.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAAQETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQIGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(22247..25354)
                     /locus_tag="ACHY09_000858"
     CDS             complement(22247..25354)
                     /locus_tag="ACHY09_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFS3883864.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(25354..26475)
                     /locus_tag="ACHY09_000859"
     CDS             complement(25354..26475)
                     /locus_tag="ACHY09_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFS3883865.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(26624..27190)
                     /locus_tag="ACHY09_000860"
     CDS             complement(26624..27190)
                     /locus_tag="ACHY09_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS3883866.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            27668..28456
                     /gene="fabI"
                     /locus_tag="ACHY09_000861"
     CDS             27668..28456
                     /gene="fabI"
                     /locus_tag="ACHY09_000861"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS3883867.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            28600..29727
                     /gene="yciW"
                     /locus_tag="ACHY09_000862"
     CDS             28600..29727
                     /gene="yciW"
                     /locus_tag="ACHY09_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFS3883868.1"
                     /translation="MEKRHITGKSYWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARSIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFATCMNSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            29795..31729
                     /gene="rnb"
                     /locus_tag="ACHY09_000863"
     CDS             29795..31729
                     /gene="rnb"
                     /locus_tag="ACHY09_000863"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFS3883869.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRTN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDLVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(31806..31910)
                     /locus_tag="ACHY09_000864"
     CDS             complement(31806..31910)
                     /locus_tag="ACHY09_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883870.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            31964..33949
                     /gene="pdeR"
                     /locus_tag="ACHY09_000865"
     CDS             31964..33949
                     /gene="pdeR"
                     /locus_tag="ACHY09_000865"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFS3883871.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(33931..34059)
                     /gene="ymiD"
                     /locus_tag="ACHY09_000866"
     CDS             complement(33931..34059)
                     /gene="ymiD"
                     /locus_tag="ACHY09_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFS3883872.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIITEYIAKYIYSSTYARFR"
     gene            34097..34270
                     /gene="yciZ"
                     /locus_tag="ACHY09_000867"
     CDS             34097..34270
                     /gene="yciZ"
                     /locus_tag="ACHY09_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFS3883873.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            34360..35109
                     /gene="yciT"
                     /locus_tag="ACHY09_000868"
     CDS             34360..35109
                     /gene="yciT"
                     /locus_tag="ACHY09_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFS3883874.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEIILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            35378..35596
                     /gene="osmB"
                     /locus_tag="ACHY09_000869"
     CDS             35378..35596
                     /gene="osmB"
                     /locus_tag="ACHY09_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFS3883875.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(35722..36048)
                     /gene="yciH"
                     /locus_tag="ACHY09_000870"
     CDS             complement(35722..36048)
                     /gene="yciH"
                     /locus_tag="ACHY09_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFS3883876.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(36048..36785)
                     /gene="pyrF"
                     /locus_tag="ACHY09_000871"
     CDS             complement(36048..36785)
                     /gene="pyrF"
                     /locus_tag="ACHY09_000871"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS3883877.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
BASE COUNT         8760 a         9712 c         9256 g         9194 t
ORIGIN      
        1 ttattgttca gttctgtcgt tatctcttta ctagagtatt ttttacggtt aatcgcacga
       61 ttatatccaa gtttttcgac acctgcgtaa tcattacgcg accccagaat gctttgaata
      121 tatatcgctg gcacaccggg gaaacttaac aaaatcgcat gggcgagaat aaacctggca
      181 caccgttctt cgtcgctaca ctcacggcgg ctcaacgcat ccatataggt gacattcatt
      241 tcataggggc tacgcgtacc gtcgggatta tttttccagt ttactaatgc cccttcctgc
      301 tgtaacacct cgaccagcgc taatatttcg ttttcgggca acaacccccg cagtgggtta
      361 agcccgatgc catcgtgaga ggcaaggaag ttaaaccagg tggtgttgct ggaaggtagt
      421 gtcaggtttt gcgcccacgc acaaagtgcc tcaacgttct gtttttgcac cgcatgcagc
      481 accagcggcg gcagcgagaa ctggtacacc atatgcgctt cgtcatcacc gttgccaaag
      541 taagcaatgt tgtctatatg cggaacattg gtctcggtaa tgatcactgt gcctggcgca
      601 acgttatcaa taatcgaccg taacagtttg ataatcagat gtgttttttc cagatggatg
      661 cagcttgttc ccggctcttt ccacataaag ccaacggcat ccagacggat atactcagca
      721 cctttctcaa ggtaacacag taaaacatcc accatcgcca gtaacacttc agggctacgg
      781 taattgaggt caatttgatc gtcgctaaag gtggtccata aatgacgcgt tgaattatcg
      841 cgcatctgga atggcgttaa taacggtaac gcacgcggac gggtgacggc gctgagatcg
      901 gtttgcggat caaccgcaat aaaaaaatct tcaaaacctg gctgctgttg taaatagttt
      961 ttaaaccatt cactttttgc cgacatatgg ttgcagacaa aatcaaacat taaatgactg
     1021 cattcaccga gttgctgaat atcctgccac tcccccgctt cactggcgac ctgatgataa
     1081 tcaattaccg aaaagccatc atcagatgac catggataaa acggcaacaa atgaacatgt
     1141 gaaaaagtgc tttgcagcca ttgatggtaa aactgattaa atgtgggtag tggttttaaa
     1201 tcattactgt gaaattgatc ggcataggta attaacacga catcactttc atcccagtgt
     1261 tttttacgtc gctgtgtaat taatcgtttc gcactctcaa gacgcgttac aagtttctgt
     1321 aaatgagtcg cggtaaatgt tccaccgtag atttcgtcca ggtaatccgt aattttctgt
     1381 tccatgttgc cttttcgtgg tagcggtccc atgaaacgag agactactcc gcagaaaata
     1441 aactgtcaat tgagcacaag ggttttagtc acgcaggaaa tcatttttta ccagggaaaa
     1501 agcgtatttg tgagctgacg caagtaatct atggggatgt aaataaggtg tgacagaaaa
     1561 agtaaacggc gcataagcgc cgcttagggt ggattctttt aacctttgac cttcggcatt
     1621 gcgatgtctt tcaggccgcc ggcgttctca acgtgggtat atcccatctc gctaaggatc
     1681 tcttttgcct gccctgactg gcgtccggca ttgcaataca ctttcacggt gtcgttttta
     1741 tccggaacgg cggtggcaat gcgctctttc acttccttca ggggaatatt gatggctccc
     1801 tgaacgtgct cttgctgata ctgctctgga acacgaacat cgatccagtg ttcagcggcg
     1861 aaaacgggca gtgaaaacac cagtgtcaga gcaagtaagc cttttttaaa catgtaagcg
     1921 tccttctatg gatgaaaatt gccatccata atacgcctta gctttaacaa tttttgcgaa
     1981 actcccctca ccttttataa cgctgcgcca gtttattggc agcccgactt aacagcggtt
     2041 ccagcgccac agccagcaac attttcagtg gtcggcgagc aactgatttt atcgcccagc
     2101 cagctacacc cgccgggcca tagcgcagtg cggtaagaag taccagcttg cctgctaatt
     2161 tgaaaccagg cttcaccttt tgcccggcct gttgccagcg agtattcata actttcctca
     2221 cagttgacgg aaacggctac gtaacgtgaa agtatcggaa gtgacataac gttccatctc
     2281 gcgtaaacgc gtttcacttg ccgccagttc acggtcgact tcatccagca attcactgct
     2341 ggaaggtagc tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg caaatgacaa
     2401 aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca gcaccaccag
     2461 gatacgcacc agttttactg gtacatcaaa atagttggca atcccggcgc agacgccacg
     2521 gaccatgccc tgctgtggaa tacgccataa ttttttattg agattaatgc ccgccattag
     2581 cgatccctcc agttcggatg ttctgcatca agaatggatt ccagcgcctg aatacgttcg
     2641 cgcatccgtt ttgcttcatc agccagttgc gctaatcgct gctgctcact ttgcgacaat
     2701 tcactgcgac cagaacgatt gctgtaatgc agccataacc agatcggtaa aacaaacagc
     2761 acaaaaatgg ttaacggaat agccagaaat agcgcgctca taaggactcc ttagacgatg
     2821 agcggcggcg cgttcggacg ccgcctggat gttattattg attgtcttgc ttcattttgg
     2881 cttttaattg tgccagttgc tcgctgattg catcatcggc tttcagttcg gcaaactgat
     2941 cgtccagcga tttttgttta ccgaagctgt ggctttctgc ttccgcttcc atctggtcaa
     3001 ttcgacgttc gaaagattcg aaacgagcca ttgcttcatc cagtttgcca ctgtccagct
     3061 gacgacgcac atcgcgcgac gagtttgccg cctgatggcg taacatcaat gcctgctggc
     3121 gagcgcgtgt ttcgctcaat ttgttttcca gctcgccgat ctctttcttc atgcgtgcca
     3181 gcgtatcgtc caccagcgtc acttcatgtt ccaggctctt aatcagatcg gtcagtttct
     3241 gtttttcaat taacgccgca cgcgccaggt cttctctctc tttcagcagc gccagttcgg
     3301 ctttttcctg ccattcaact tcacgcgccg acgcttgttc aatacggcga gtcagctgtt
     3361 tcttttctgc cagcgcacgc gccgaagtag aacgtacttc aaccagtgta tcttccatct
     3421 cctggatcat cagacgcacc agtttctgtg gatcttccgc tttctctaac agagcgttga
     3481 tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg tcctcttgat
     3541 ttctgcgttc aggattgtcc tgctgaacta ttaatacaat ttgcgtgcca attttttatc
     3601 tttttgattt ataaagatct gattgaagaa tcaacagcaa catgccagga tgagttagcg
     3661 aattacacta acaagtggcg aatttcatca tggcagaata caaagataat ttactcggtg
     3721 aggcgaacag ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg ctggataaac
     3781 cggtgctcat catcggcgag cgcggcacag gtaaagagct gattgccagc cgtctgcatt
     3841 atctctcctc ccgttggcag gggccgttta tttcccttaa ctgcgcggcg ttaaatgaaa
     3901 atctgctgga ttccgaactg tttggtcacg aagcgggggc gtttaccggt gcgcaaaaac
     3961 gtcatccagg gagatttgaa cgtgccgacg gcggtacgct atttcttgat gaactcgcta
     4021 cggcaccgat gatggtgcag gagaaattat tgcgcgtgat tgagtacggt gaactggagc
     4081 gcgttggcgg cagccagcca ttgcaggtga atgtacggtt ggtatgcgcg acgaatgccg
     4141 atctcccggc gatggtcaat gaaggtactt ttcgcgctga cctgctcgac cggctggctt
     4201 tcgatgtagt gcaactgccg cccctgcgcg agcgcgaaag cgacattatg ctgatggcag
     4261 aacactttgc cattcagatg tgtcgggaaa tcaagctgcc tctgttcccg ggttttacgg
     4321 agcgcgccag agaaacattg ctgaattatc gctggccggg aaatattcgt gaattgaaaa
     4381 acgtggtgga acgttcggtg tatcgccacg gcaccagcga ttatccgctt gatgacatca
     4441 ttattgatcc ctttaaacgg cgtccgcctg aagacgctat cgccgtttca gaaaccacct
     4501 cgcttccaac gctgccgctg gatttacgtg agtttcagat gcagcaggaa aaagagttgc
     4561 tgcaactcag tttgcaacaa gggaaatata accagaagcg cgcggctgaa ttactggggt
     4621 taacctatca tcagttccgc gcgttgttga aaaagcacca gatttagcga agttgggcaa
     4681 tacaacttgc cggatgcggc gtgaacgcct tatccggcat acataatcaa tacgttgttt
     4741 gcaggcctga taagcgcagc gcatcaaaca ttattgcgtt gggctattaa tcgctcagcg
     4801 catcctgcaa aattttcatt gcctcatcga attgcgcatc cgggatggtc agcggataca
     4861 ggaagcgaat cacgttgccg tatgcgccac aggtcagcaa gagcagcccc tgcgccagcg
     4921 cgcgttgctg gattttctgt gcaatcgccg ctgacggctc gcccgtttgc ggatcgttaa
     4981 actctgccgc aatcatagac cccagaccgc gtaccgccgc gatggccgga acgctttctt
     5041 tggcatcaat caacgtgttt gtcaggcgct ggcccagttg attcgcgcgt tcacagagtg
     5101 attctttgtc gataatgttg agcacagcgt gcgcggcagc caccgccagc gggttcccgg
     5161 cgtaggtacc acccaacccg cccggcgcgg gcgcgtccat aatattcgcg ttaccgacca
     5221 cgcccgaaag cggcatcccg cctgcgaggc ttttcgccat cgtcattaag tccggcttat
     5281 cggcgtagtg atccatggca aacattttac cggtacgcgc aaagccgctt tgcacttcat
     5341 cggcaatcat cacaatgccg tgctcgtcgc acaggcggcg aatggcggca accagctctt
     5401 ttggcgcaac gttgaaaccg ccttcgccct gaaccggttc gaaaataatc gccgccacct
     5461 gcttcgcttc aatgtccgat ttaaacaagc gttcgatggc gtcgagggag tcctgcgttg
     5521 aaatgccgtg taaatctgac ggataaggta cgtgatatac cgaaccgggg aacgggccga
     5581 agccgatttt gtacggcgcg acctttccgg tcagcgccat ggtcatatat gtacgaccgt
     5641 ggaagccgcc gctaaacgca atcacgccag ggcgtccggt atgggcgcgg gcgattttca
     5701 ccgcgttctc caccgcttcc gcaccggtgg tgaagaacgc agttttagcc tgcccgctca
     5761 ccggggcaag ggcgttaatt ttctccgcca gcgtgacgta gctttcgtac ggcacaatct
     5821 gatacgccgt gtgggtaaac tgttgcaatt gctgttccac cgccgcgacc agatcagggt
     5881 ggcgatgccc ggtattcagt accgcaatgc ctgcggcgaa atcgatatat tcgttgccct
     5941 ctacatcctt cagcgtggcg ttttctgccg actgggcgaa gaagttacac atcacgccaa
     6001 ccccgcgtgg ggtagcagaa agacgacgct gatggaattc attgttgctc atgtttcaca
     6061 ccttttgact cttcactgtt aatcggaaat taaacgccca gacgatcgcg caggctgtaa
     6121 taagccgcgc ccatcgcggt aaacggaacg cgcagcgtgc gcccgccggg gaacgggtaa
     6181 tgcggcagat tggcgaaggc gtcgaaacgc tcggcgtcac cgcgcagcag ttcagcaatc
     6241 aaacgcccgg cgaggtgggt acaggtgacg ccgtggccgc tgtagccctg catgtagtag
     6301 atgttggtat cgaggcgacc aaactgcggc atgcgtgaca gggtcagcag gaagttgccc
     6361 gtccagcggt aatcaatttt cacgcccttc agttgcggga aggttttcag cagtttcggc
     6421 accacaaggc gctcaacatc gtccgggtcg cgtgcgccgt agaccacgcc gccgccgtac
     6481 agcaggcggt tatcggcggt aagacggtag taatcaagca gatagttaca gtcttccaca
     6541 cagtagtttt tcgggatcag cgaacgggct aaatcttccg acagccgttc ggtggtgatc
     6601 acctgcgtgc cgcacggcat gctgcgtttc gctagttccg gctctacttt atcgcccagg
     6661 taggcattcc cggcgacaat cacatacttc gccgtcacct gaccattggc agtccgcacc
     6721 acggctggcg tggtgtgctg gatctgcgtc actgcggaaa gctcatgcac gcgcccgccg
     6781 ttcaggcgga tggcatcggc ttcaccaatg gcgaggttta gcgggtgaat atgcccaccg
     6841 ctgtgatcca gcagcgcacc agtgtagcga tcgctggcga cctcgcggcg aatagcgttg
     6901 gcgtccagca gctccagctg tttattgccg tagcgttccc agttctcttt ctgctcttcg
     6961 agtgtggcga gctgtttatc attcatcgcc acaaacagac cgccggggcg atagtcgcag
     7021 tcaatctgat aacgtttgat acgttcgcgg atgatctcgc caccctcgaa catcatgctg
     7081 ccgagcatac gggcggtatc catgccgtag cttttttcga tcacgtcgat gtcgcggctg
     7141 taggagttca caagctgccc gccgttgcgt ccgctcgcgc caaagccgat gcgtgaggct
     7201 tcgagaacca ccacgtcaaa gcccgcttcc gccagatgca gcgcggagga aagcccggta
     7261 tagccgccgc cgaccacgca aacgtcgcag gaaatcgact cattcagcgt gtcgaatggc
     7321 gcgtatttat tcgcactggc ggcgtagtaa ctgctggtgt gttcggtcat ttcaggcctc
     7381 caggcttatc cagatggttt tcagttcggt gaatttttca agggcatgca gggatttgtc
     7441 gcgcccgttg ccgctctgct tatagccgcc aaacggcacg gtcatatcgc cgtcgttgta
     7501 gttattgacg aagacggagc cggctttcag gcggcgactc atgcggtgcg cgcgggagag
     7561 gtcgcgcgtc cataccgccg cgccaagtcc gtactggctg tcgttggcaa gctgtagcgc
     7621 ctgttcttct gatgtgaaac gcgtgaccac cagcaccgga ccgaaaatct cttcgcggct
     7681 taaggacgca ttcgggtcta cctccacaaa gatggtcggg ccgatggcgg cagccagctc
     7741 ggcgttacgg ccatccagca gcagttgccc tttgctttcg ccttcccgaa taaagctatg
     7801 gaccgagtcg gcgtgggcgc agtcgattaa ggtgcccatg gtggttgcgg gatcaagcgg
     7861 atggcccggc tgccagtttt gcgcttgctg ttttaacagg gctaagaatt catcggcgat
     7921 gctctcttcc agcaacaggc gcgttccggc gatacacacc tgcccctggt tgtagaaaat
     7981 gcccgcagcg gtcgcgctcg ccgctttttg caaatccgga cagtcggcga aaacgatgtt
     8041 ggcgcttttg ccgcccgctt ccagccagac gcgtttcatg ttgctgtcgc ccgcgtcctt
     8101 cagcagctgt ttcccggtgc gggttgagcc agtaaaggca atggcatcga tatcgttatg
     8161 gcgcgaaagc gcctgcccgg cttcatgacc aaaacccgtc accacgttca acacaccatc
     8221 cggcagcccc gcttctttcg ccagccccgc gagacgaatc gcactgagcg gtgatttttc
     8281 agacggtttt agaatcacgc tgtttcccgc cgccagcgcc gggccgagtt tccagcaagt
     8341 cagcagcagc gggaagttcc acggcacgat ggcggcaatc acgccgaccg gttcacgcac
     8401 gatcatcgcc agctcatggc tactggtggt cgccacttcg ccatacactt tgtcgatcgc
     8461 ttcggcgtac cagcgaatgg cgcgcgccgc gccgggaata tcatcacgca ggctgtgacg
     8521 aatcggttta ccggtgtcga gcgtttccag cagcgccagt tcttcagcgt tggcttccat
     8581 taaatcggcg agtttattca gcaccgcttt acgcttagcc ggagaagaga gtgaccagtc
     8641 gccacgttca aacacgccgc gtgctgcgct caccgcacgg tcaatatcga cgctcttgcc
     8701 gcgggcaata ttcgccagcg gtgcctgggt gaccggatca acggtttcaa aagtttcatt
     8761 ttccgccgca gcagtatatt caccgttaat aaataagcgg gtttcaatgg cgagacttaa
     8821 cgctttatcc tgccagtaag ccagatgatg aaaattcatt atgactcctg tttcacgtct
     8881 atcagatata tgcagtttgt gaacgtcagg gcgcagagat ccccgggccg tatgcccgtt
     8941 tatttctggc gacattcttt atagattgcc gggcgcattg cgcttacccg gcttacagaa
     9001 caaaaagatt aaaacgtggt gggcgtatgg gcgctgataa ttcggcaaat acctgccgac
     9061 gtattactga aactgtgcgg gatgccggta ttaatggcat aactttgccc cgcgacgaga
     9121 tgataatcct gaccattaat cgtcagaaca atttcacctt ccagtacagt gcctatttcc
     9181 tcaccctgat gcttaattct ttccccagtg gttgtgcccg gctggtaagt ttcaaagatc
     9241 atcgccagcg tgcgattcgg gttaccgtta tgaaccagct tcattgacac accctgactg
     9301 cccatctcaa ttaagtcgtc ctgattaatg acgacctgcg gctcatcagg tttttccggc
     9361 tcggaaaaga attccgagag tgacagacca tacaccttca gcagcttttg cagcgtactg
     9421 atggcagggc tgactttatc ttgttctatc gtactgatag cactgtgagt cagcccggag
     9481 agttcggcgg cacgacgttg tgaaagcccc tgctgctggc ggatttccga caagcgtttt
     9541 cctggcgcca gtccctcatc actcatattt gcatttcctt aaactgtagt ggtcagagtc
     9601 gctgtttttc agcgatatgg tgctgacaag cggtgataaa gccctcgaac aatatacgcg
     9661 aaagcgcgta ctcgctactg ttccattccg ggtgccactg tacgcccagc gcaaaaggat
     9721 gattgatgac gctaaccgcc tcaactaagc catccggcga gcgagcttca acacgcaacc
     9781 gtgggctaac gaccttcgcc ccttgtccat gcagagagtt tacccaaaag ttgctacatt
     9841 caggtaacaa cgcagacagt aatcccccct cttcaacctg aacttcatgc gacggcgcat
     9901 attgctgctc caccggcagt tcgggatctt cccgatgttc cagcaattca ggctgttcgc
     9961 acagcttgcg atgcaacgaa ccaccggttg ccaccaccag ttcctgtaaa ccccggcaga
    10021 tggcgaaaat ggggatgcgc ctttcgagtg cggcattaat taacgccatg ctcagaagat
    10081 cacgcccagg atcggcgtca ggctcatcgc cgttttcacc atataggtgc ggctgcacat
    10141 tgctgggact accaggaaga taaatgccat cgagtttcgg caaaagttgt tcaagtaatg
    10201 acggttccgc cagcgcatgt ggcagcgcaa ttggcaagcc gcctgcgtgg atgatggcat
    10261 tcaggtactt ttcttgcaga gtctgggtcg catgaccctt aagcctgttc ctgcacatta
    10321 cgacaccgat aaccggattg ttcattatat tttccatgct caatctcaca aagtggacta
    10381 aattatcgcc attactgcgc tttaaatgac ctttatgttc aatatttttt caatctagca
    10441 gtgggttttc atttttgcaa actcaattta acatttgaca aacatttagt ttgcatacag
    10501 attcgaatag tggtcattat attttacgct ttgataacga gcggaaaaca aaccaaaggc
    10561 gaagaatcat ggaaaccaat atcgttgaag tagagaactt tgttcagcag tcagaagaga
    10621 ggcggggtag ctcctttacg caggaagtga aacgctacct ggagcgctac ccgaatacgc
    10681 aatatgttga tgttttactt accgatttga atggttgctt ccgtggaaaa cgtattcctg
    10741 tttcaagcct gaagaagctc gagaaagggt gttatttccc ggcctcggtg tttgcaatgg
    10801 atattctggg caacgtagta gaagaagcgg gcctgggtca ggaaatgggc gagccggatc
    10861 gtacctgtgt tcctgttctc ggttccttaa ccccttctgc cgccgatcca gagtttatcg
    10921 gtcagatgct cctgaccatg gtcgatgaag atggcgctcc ctttgacgtt gagccgcgga
    10981 acgttcttaa ccgcctgtgg cagcagctgc gccagcgcgg attgttcccg gtcgtagcgg
    11041 tagagctgga gttctattta ctggatcgcc agcgcgacgc tgaaggatat ctgcaaccgc
    11101 cctgcgcgcc cggcaccgat gaccgcaata cacaaagcca ggtttactcc gttgataacc
    11161 tcaaccactt cgccgacgtg ctcaatgata ttgatgagct ggcgcagtta cagctgattc
    11221 cggcagatgg cgcggtcgcc gaggcctcgc caggtcagtt tgaaatcaat ctttaccata
    11281 ccgataacgt gctggaagct tgcgatgatg cgctggcact aaaacgcctg gtgcgtctga
    11341 tggcagaaaa gcataagatg cacgccactt ttatggcgaa gccgtatgaa gagcacgcgg
    11401 gcagcgggat gcatatccat atcagtatgc aaaataatcg tggcgagaac gtgctttctg
    11461 acgcggaagg cgaagattcg ccgctgctga aaaagatgct cgccgggatg attgacctga
    11521 tgccgtcatc aatggcgttg ctggcaccaa acgtgaactc gtatcgccgc ttccagccgg
    11581 ggatgtatgt accgacgcag gcgtcgtggg gccataacaa ccgcaccgtc gccctgcgta
    11641 ttccctgcgg cgatcgccat aatcaccgcg tggaatatcg cgtggcgggt gccgatgcca
    11701 acccgtatct ggtgatggcg gcgatttttg ccggtatttt gcatggcctt gataacgaac
    11761 tgccattaca ggaagaagtc gaaggtaacg ggctggaaca ggaaggctta cccttcccga
    11821 ttcgccagag cgatgccctg ggtgagttta tcgagaatga tcacctgcgc cgctatttag
    11881 gcgaacgctt ctgccatgtc tatcacgcct gtaaaaatga tgagctgttg cagtttgagc
    11941 gcctcatcac agaaacagaa attgagtgga tgttgaaaaa cgcgtaatac cccttgcggg
    12001 gttgtttcat cggcctgagc ggagccaggc ctgacatttg ctggtaatcc agacatttcc
    12061 cggatgtcgc gttatggagc gcaggcggca acgggcttcc gttaacgatt aagactgtga
    12121 cgaggaaatg agatgatgcc gatgattaaa tccccgcatg gcgagggagg ctgcgtatgc
    12181 gcccctcctg cgactgactg gactccgccc cctctgcttc ccttgctaaa caggtttgat
    12241 ttcaggtcga cacgaccgca aaccttatta cgcaggggag gcagcaatta tggctattaa
    12301 ttcaccactg aatattgctg cgcaacctgg caaaacccgt ctgcgaaaat cactgaaatt
    12361 gtggcaggtg gtgatgatgg gtctggccta tctcacgccg atgaccgtat ttgatacttt
    12421 tggtattgtc tccggcatta gcgacggtca cgttccggcg tcctatttgc tggcgctggc
    12481 gggcgtgctg tttaccgcta tcagctacgg caaactggtt cgccagtttc cggaggccgg
    12541 ttcggcctat acctacgcgc aaaagtcgat taacccgcac gtcggattta tggtcggctg
    12601 gtcatcgctg ctggattatc tctttttgcc gatgatcaac gttttgttgg cgaaaatcta
    12661 tctctccgcc ctcttcccgg aagtgccgcc gtgggtgtgg gtggtaacct tcgttgccat
    12721 tttaaccgcc gcgaatctga agagcgtcaa cctggtcgct aacttcaata ccttgtttgt
    12781 actggtgcaa atctccatca tggtggtgtt tatcttcctg gttgttcagg gactgcataa
    12841 aggagaaggc gttggcaccg tctggtcact tcagccgttt atcagcgaga acgcgcacct
    12901 gatcccaatt attaccgggg cgacgattgt ctgtttctcc ttcctcggtt tcgatgcggt
    12961 gaccacgctt tcggaagaga cgccggacgc cgcacgcgtg atcccgaaag ccatcttcct
    13021 gacggcggta tacggcggcg ttatctttat cgctgcgtcg ttctttatgc agctgttctt
    13081 tcccgatatc agccgcttta aagacccgga cgccgcactg cctgaaattg cgctatacgt
    13141 cggcggcaag ctcttccagt cgattttcct ctgtaccacg tttgtgaaca cgttagcgtc
    13201 tggcctggcg tcgcacgcca gcgtgtcgcg tctgctgtat gtgatggggc gtgacaatgt
    13261 gtttccggag cgcgtgtttg gctatgtgca cccgaaatgg cggactccgg cactgaacgt
    13321 cattatggtc gggattgtcg cgttgtcagc gctgttcttt gatttagtca ccgcgacagc
    13381 attgattaac ttcggtgctc tggtggcatt taccttcgta aatctgtcgg tgtttaatca
    13441 tttctggcgg cgtaaaggaa tgaataaaag ctggaaggat cacttccact atttgctgat
    13501 gccgctggtt ggcgcgctga cggtgggtgt gttgtgggtt aacctcgagt caacgtcact
    13561 gacactcggt ctggtatggg cttcgttagg cggcgcatat ttgtggtatc tgatccgccg
    13621 ctatcgcaaa gtgccgttgt atgatggtga cagaacgccg gtgagcgaaa cgtaatagtc
    13681 ggatgcgcgg cgcattatcc tgcctgaggg taatgcaccg cgccattaac tataaaaaga
    13741 tgtatgaccc ggatcacgag tgctatcatc cgcccttatg cttagcgcat taaccccagg
    13801 ggcaaataat gaaccacgat atcccgctaa aatattttga tattgccgat gagtacgcga
    13861 ccgaatgcgc agagcctgtc gcagacgctg aacgcacacc gctggccctc tacttccagt
    13921 tactgctcac ccgtttgatg aacaatgaag agatcagcga agaagcccaa catgaaatgg
    13981 ctgctgaagc ggggattaat cccgtacgca ttgatgagat cgcagagttt ttgaatcaat
    14041 ggggtaatga gtaattattt cattgcagac tctcggtcag tatcgttgtc cggataaagc
    14101 gttcacgccg catccggcta acaatgacac gcactgcagc gtgcctcaat gggtttctca
    14161 cgatcgtacc gctctgctcc tcgctaagtt gttctgtgag ttatagttat agagaaatca
    14221 cgggtacgaa tgttgaacag atggcgacgt catctgttca acgaccaacc acacggccct
    14281 cattacgacc cctgaacaca aacggtaatg gcaaatgtca ttaaatatta gtaagatagc
    14341 ctctttacat aaaatccatt aatattatgc gccaggtatt atcgtctctt ttggtgattg
    14401 ctggacttgt gagtggtcag gcaatcgccg cgcctgaatc ccccccgcat gctgatatcc
    14461 gcgacagcgg ttttgtctat tgcgtcagcg ggcaagtcaa cacctttaac ccatccaaag
    14521 cgagcagtgg gttaattgtc gatacccttg ccgcccagtt ttatgatcga ctgctggatg
    14581 tcgatcccta tacctatcgc ctgatgccgg aacttgccga aagctgggaa gtactcgaca
    14641 acggcgcgac ctatcgcttc cacctgcgtc gcgatgttcc gtttcaaaaa accgactggt
    14701 ttactcccac tcgtaaaatg aatgccgacg atgtggtgtt taccttccag cgaatttttg
    14761 accgcaacaa cccgtggcat aacgtcaacg gcagcaactt cccctacttc gacagcctgc
    14821 aatttgccga taacgtgaaa agcgtccgta aactggataa tcataccgtt gagttccgtc
    14881 tggctcagcc ggatgcttct tttttgtggc acctcgcaac ccattatgct tcggtcatgt
    14941 cggcagaata tgcccggaag ttagagaaag aagatcgcca ggagcaactc gaccgtcagc
    15001 cggtcggcac tggaccgtat cagttgtcgg aataccgcgc cgggcaatat attcgcttac
    15061 aacgtcatga tgatttctgg cgtggtaagc cgttgatgcc acaagtggtg gtggatttag
    15121 gctctggcgg tacaggacga ttgtcgaaac tcctgaccgg ggagtgcgac gttctggcct
    15181 ggcctgctgc cagtcagcta tccattttgc gtgacgaccc acgcttgcgt ttaacgctgc
    15241 gcccggggat gaacgtcgcc tatctggcgt ttaacaccgc taaaccgccg ctaaataacc
    15301 ccgctgtccg ccatgcgctg gcactggcga ttaataacca gcgcctgatg caatccatct
    15361 attatggtac ggctgaaacg gcggcctcta ttttaccgcg cgcctcgtgg gcctatgaca
    15421 acgaggctaa aattactgaa tacaatccgg caaaatcgcg cgaacagttg aaggcgttgg
    15481 ggctggaaaa cttaacgctg aaactgtggg tgccaacacg ttcgcaggcg tggaacccca
    15541 gtccgctgaa aaccgccgaa ctgattcagg cggatatggc acaggttggc gtaaaagtgg
    15601 tgattgtgcc ggtagaaggt cgttttcagg aggcgcggtt gatggatatg agccatgatc
    15661 tgacgttatc cggttgggcg acggacagta acgacccgga cagtttcttc cgtccgttac
    15721 tgagctgcgc ggcaattcat tcacagacca acctcgccca ctggtgcgat ccgaaattcg
    15781 acagcgtgtt gcgtaaggcg ctctcctcgc agcagctggc ggcgcgtatt gaagcctatg
    15841 acgaagcgca gagtattctg gcgcaggaat tacccattct gccgctggcg tcgtcattgc
    15901 gcttgcaggc ctatcggtac gatatcaaag gtctggtact tagcccgttt ggtaacgcct
    15961 cctttgctgg ggtgtatcgc gagaaacagg atgaggtgaa aaagccatga ttatcttcac
    16021 cttgcgccgt attttgttat tgattgtcac cttgttcctg ctgaccttcg ttggcttcag
    16081 cttgagctat ttcaccccac atgcgccgtt acaaggcgcg tcactgtgga atgcctgggt
    16141 gttctggttt aacggcctga tccactggga ttttggtgtt tccagcatta acggtcaacc
    16201 gattgccgaa cagttaaaag aggtcttccc ggcgacgatg gaactgtgca tcctcgcctt
    16261 cggttttgcg ctgattgttg gcatccccgt aggaatgatt gccgggatta cgcgccataa
    16321 gtggcaggat aacttgatca acgccatcgc cttactgggc ttttcaatcc cggtgttctg
    16381 gctggcgctt ctgctgacgc tgttttgttc actcacacta ggttggttgc ccgtttcagg
    16441 gcgtttcgat ctgctctacg aagtgaaacc gattaccggt tttgcgttga ttgatgcctg
    16501 gctttcggac tcaccatggc gggatgaaat ggtcatgagc gcaatccgcc atatgatatt
    16561 gcccgtgatc accctgtcgg ttgcgccaac aactgaagtg atccgactga tgcgtattag
    16621 cactatcgaa gtgtatgacc agaactatgt taaagcagcg gcgacccgcg gtttgtcgcg
    16681 ctttactatt ttgcgtcgcc acgttctgca taacgcgtta cctccggtta ttcctcgcct
    16741 gggcttacag ttttctacca tgctgacgct agcgatgatc accgaaatgg tttttagctg
    16801 gccgggttta gggcgctggt taattaacgc catccgccag caggattacg cagccatttc
    16861 cgccggagtg atggtgtgtg gctcactggt cattattgtt aacgtgattt ctgatatttt
    16921 gggtgccatg gctaaccctc tgaaacataa ggaatggtat gccttacgat agcgtataca
    16981 gcgaaaagcg cccgccgggc acgctgcgta ccgcctggcg caaattttat agtgacgcct
    17041 ctgcgatggt cggcctgtac ggctgcgcgg ggctggctgt actgtgtatt tttggcggct
    17101 ggtttgcgcc ttacggcatc gaccagcaat ttctcggtta ccaattattg ccgccgtcat
    17161 ggtcgcgcta tggcgaagtt tctttcttcc tggggactga cgacctcgga cgcgatgttt
    17221 taagccgttt actgagcgga gctgcgccaa ccgtaggtgg cgcatttgtg gtgacgcttg
    17281 ccgcgacgat ctgtggcctg gttctgggca ccttcgccgg ggcgacccac ggcctacgct
    17341 cagcggtgct taaccatatt ctggatacct tactggcgat cccttcgctg ctgctggcaa
    17401 ttatcgttgt agcgtttgcc ggaccgagtt tgtctcacgc catgtttgct gtctggctgg
    17461 cattgctgcc gcgtatggta cgttcgattt acagcatggt gcatgacgaa ctggaaaaag
    17521 agtacgttat cgccgcccgt ctggatggcg catcaacgct gaatattctc tggtttgctg
    17581 tgatgcccaa catcaccgcc gggctggtca ccgagatcac ccgcgcactg tcgatggcaa
    17641 ttctcgatat cgccgcgctg ggctttctcg atctcggcgc acaactcccc tcgcctgaat
    17701 ggggagcgat gctcggtgat gcgctggaac tgatttatgt cgccccgtgg actgtcatgc
    17761 tgccaggtgc ggcaattatg attagcgtgc tgttggttaa cctccttggc gacggtgttc
    17821 gtcgtgcaat tattgcgggg gtggaataat gccattactc gatattcgta acctgaccat
    17881 tgaatttaaa accggtgatg agtgggttaa agccgtcgac cgcgtaagta tgaccttaac
    17941 cgaaggtgaa atccgcggtc ttgttggcga atccggttcc ggcaaaagtt tgattgcgaa
    18001 agcgatttgt ggggtgaata aagataactg gcgtgttact gctgaccgta tgcgttttga
    18061 tgatatcgat ttgctgcgtc tctccgcacg cgaacggcgc aagctggttg gtcacaacgt
    18121 gtcgatgatt ttccaggaac cgcagtcttg tcttgaccct tcagaacgtg tgggtcgcca
    18181 gttgatgcaa aacatcccag cctggacgta taaaggccgt tggtggcagc gttttggctg
    18241 gcgcaaacgc cgtgccattg aactactgca ccgtgtgggg attaaagatc acaaagatgc
    18301 gatgcgcagt tttccctatg agttgaccga aggtgaatgt cagaaagtga tgatagccat
    18361 tgcactggcg aatcaaccgc gtctgctgat tgctgacgaa ccgacaaact caatggagcc
    18421 aacaacccag gcgcaaatct ttcgcctgct gacgcgtctc aaccagaaca gcaataccac
    18481 tattttgctt atcagccatg acttacaaat gcttagccaa tgggcggata aaattaacgt
    18541 gctttactgc ggtcaaacgg tggaaaccgc gccaagtaag gagttggtga caatgccaca
    18601 tcatccttat acccaggcgc tgatccgcgc gataccagac ttcggcagcg cgatgccgca
    18661 taaaagtcgc ctcaatacgc tgcccggcgc tatcccactg ctggaacagt taccgattgg
    18721 gtgtcgtctg gggccgcgtt gcccgtatgc acaacgagaa tgcattgtga cgccacgttt
    18781 gacgggggcg aaaaatcatc tctatgcctg tcacttcccg ctgaacatgg agaaagagtg
    18841 agatgatcga aacgctgctt gaagtgcgta atttaagtaa aacgttccgt taccggaccg
    18901 gctggtttcg tcgtcagacc gtagaagcgg taaaaccttt gagctttacg ctacgtgaag
    18961 gccagacact ggcgattatt ggtgagaatg ggtcgggtaa atccacactg gcaaaaatgc
    19021 tggcgggaat gatagaaccg accagcggcg agttattgat tgacgatcat ccgctgcatt
    19081 ttggcgatta ttccttccgt agtcagcgta ttcgcatgat ttttcaggat ccttcgacct
    19141 cgttgaatcc ccgtcaacgt atctcgcaga ttcttgattt tccactgcgc ttgaacaccg
    19201 atctggaacc tgaacagcgt cgcaaacaga ttattgaaac gatgcggatg gttgggctgc
    19261 taccggatca cgtcagttac tatccgcata tgttggcgcc cggacaaaaa caacgtctgg
    19321 gtctggcacg cgcgctgata ttgcgtccaa aagtgattat tgccgatgaa gccctcgcct
    19381 cactggatat gtcgatgcgt tcgcagttga ttaatctgat gctggaatta caggaaaaac
    19441 agggtatttc gtatatttat gttacccagc atatcggaat gatgaagcac attagcgacc
    19501 aggtactggt gatgcatcag ggcgaggttg tcgagcgagg cagcaccgcc gatgtgctgg
    19561 catcgccgct gcatgaactc accaaacggc ttattgccgg tcattttggc gaggcattga
    19621 cggcggatgc gtggcgtaag gaccgttagt ggtttgcacc tcatcctgac agggcccccc
    19681 tcaggatgag gtgttcatta cttttgattg tcgattctgg tgagagcaac cagcaatgcc
    19741 agcgtataac acaccgtaat tgccagactc atttttaaca gtgtttctcc gccaattccc
    19801 gacaatggcg cggctatacc gccgaagaca aacatgctca tccccatcaa cgcagacgcc
    19861 gttccagatt cctgcgtccc tactgcactc atagcctctg ccccgccaac agtgccgatg
    19921 ccgctacaaa atgcgatggt gaaaaataat gccaccagtg ccggaatggg caattgtatc
    19981 catgcgcata agaccgtgag caatgcacac aaaattgccg ctatcaggcc acctcgcaac
    20041 aatgtcatcg cgttaatccg tcgcgccagg cgcgagaaga tccatgaagc aatgataagt
    20101 ccgatgccgt taaggccaaa caccaggcta aattgcattg gactaaagcc aaattccttc
    20161 tgcaacacga acgacgaaga gccgatatat gcaaacaaac cagccagcat aaaactttga
    20221 atcaggcaaa agcgtataaa acggcggttc tgcaccacgc ttcgtccggt taatagcaat
    20281 gatgagcccc ttttattttc tggcaaggtc tcattaataa ataacaggca gcccagcaac
    20341 agtacggtgc taatttcagc cattacccag aataaagtgc gccagtcaaa agtgctaaca
    20401 atgtacccgc ccagcactgg cgacaacacc ggtgccaggc cattcaccgt cattaacagc
    20461 gcaaaaaact gggtcaacgt tactccctga tatttgtcac gagcaataga acgagagagc
    20521 accgaaccac ccgcccccgc gatcccttga ataaaacgcc agaccaccag ccagtaaata
    20581 ttgttcgttg tcgcgcataa aatggaagaa acaataaaca atagcaacga caagattaac
    20641 ggacgtttac gccctatttt gtcgctcaga gggccaaata acagttgtcc gacgccaaga
    20701 ccaatcagtg atgcggtcag ggttaattgc gttatggtcg tggttgccgc cagttgctca
    20761 gacatctccg gcaaagccgg caaataaaga tcggtacaca tcgggccgat accggctaaa
    20821 agaccaagaa tcaatgccca tgaaagagag actctagcca tattattcct tacttgagtg
    20881 atgacatacc gccgcccagc gactgccaga gcgtgattcg gtttgtaaaa tcagtcagtt
    20941 gcagtgccag cagctcctgc tgtgcgctcc acaagctgcg ctgcgccgtc aacacggtca
    21001 ggtaatcacc aacacccgcc tgatagcgac gcaagccaac atcgaccgtt tgttgctcag
    21061 ctttaacata ctggcgctgt gcatccagtt gctcttcaag cgtggtccgc cgcgccagtg
    21121 cgttagcgac atctttaaat gcgctctgaa cggttttttc ataggtggca atgagcccac
    21181 gcttttgtgc ttctgcgtaa cgaagctgcg ccagattact tccgccagtg aacagcggta
    21241 atgtgaccga tggcgcaaat gaccagatct gcattccgtg gctgaacaac gacgacaatg
    21301 cgtcactgcc aacaccggca ctggcagtca gcgaaatcgt cgggaaaaag ttagcccgcg
    21361 ccgcgccaat atctgcattg gcgctcttca aattatgttc agcttcctgg atatccgggc
    21421 gacgcagcag aacatcagat gatactcccg caggaaccag acttatcatt tgttccggca
    21481 ggctttccag tgttcccggt agcagatttt ccgcaagcgt tgtacctgcc agtaagttca
    21541 gcgcattttt atcctgcatc acttgtgtct gatagctggc aacgctggcg cgcgcttgct
    21601 gataaacact catcgcctca ctgacatctg tcgctgccgc cgtgccaatc tgttgctggc
    21661 gctggataat tttcaatgag ttttctgcac tggtcatcgt ttcttttgcc agggccaggt
    21721 tgctgttatc agccgccagt gtcagccagg cggtgctgat ttcagcaatc agcgttaaac
    21781 gagtgttctg ggctgtaaat tcactggcaa gccaggtttc ctgcgccgcc cgagataaac
    21841 tctgattacg accaaacaga tctaacgtat aactggacac cgtgccgtca gcctcggcag
    21901 ttgttcccgt accattcgcc agcgaacgac tgcgagtcga acttaaggcc gcattcaccg
    21961 tcggaaataa cgacgcatta gtttgcccat agagcgcccg tgctgagtca atatcagcaa
    22021 tcgctttttg cacatcgcgg ttactgttca gtgcaatggt tactacctgc tgcaatcggg
    22081 gatcgtggat cacctgctgc cagtcatggc tgattgcctt cccctggccc tgtgctcccg
    22141 gcaaggtcgc cgggattggg gattcaggcg tactgtagtg gggatcgagc gagacacatc
    22201 ccgcactcaa caacaccaga aaaataagag aacgacgcaa cataacttac tcctgacggc
    22261 gcggtttacc ggcaaacaaa cgtttcacca gtacaaaaaa cagaggaaca aaaaaaatag
    22321 ccagtaatgt agcggtcagc gtgccgccaa taatgcccgt accaatggcg atgcggctgt
    22381 tcgcccctgc accggttgct atcgccagcg gcattacccc cgcaataaac gccagcgagg
    22441 tcatgatgat tgggcgtaaa cgagtctgcg ccgcgcgtaa tgccgcacga ctcaaggaat
    22501 agccctctgc aaccgccgct tcagcaaatt ccacaatcag aatggcgttt ttcgacgaca
    22561 agccgatagt ggttaatagc gccacctgga agtaaacatc gttgtttaaa tcacgcatcc
    22621 aggccgccag cgccgcgcca agaagcccca gagggatcac cagaatgacc gagaacggga
    22681 ctgaccagct ttcgtacagt gctgcaaggc agaggaaaac caccagaata gaaactgcat
    22741 acagactcaa tgcctgaccg ctggccagtt tctcctgcaa tgacaaacca ctccaggccc
    22801 aggttgttcc cgcaggaaga ctgtttgcca gttcctccat ttttgtcatt gcatcgccgg
    22861 aactaaagcc agtcgcattt tccccctgaa tttcataggc tgcagaaccg ttgtagcgca
    22921 ccagtctttc cggtccatac agccagcggg tggtcgcaaa agcagagaat ggtgtcatgg
    22981 cgttatcgct accgcgcaca aaccatttac ctaagtctga cggcgcggaa cgaaattcac
    23041 tgtcgccctg aatgtagact tttttcacac gaccacggtc aataaagtca ttcacataag
    23101 taccgcccca cgcactggac agggtgtcgg tgacatcatt caaactcaac cctaatgaca
    23161 ccgctttatt actatcaata tctacctgca attgcggcat ttgtggcaaa tcattggcac
    23221 gcacagaatg taactccgga ctttgattcg ctttttctat caactgatta cgcatttgca
    23281 gtagtgtttc gcgatcggtt ccaccgttag ctaacaattc aaacgtaaaa ccattgcttt
    23341 ggcccagccc atcaacggct ggcggcgtca tcgcaaatac cgtggcatca cgaattgtgc
    23401 ccagctcttt ggtagcccgt agggcgatag cctgggcggt gttttctgcc cctttacgtt
    23461 gagaccagtt tttcaacgaa acaaacgcca tcccggtgtt ctgtccgctg ccgctaaaac
    23521 tgaaaccatc aacggtaaaa atgacatcgg tatttgcttt ctcgttaatc aggaaccagt
    23581 caacaatctg gcgattcact tctgctgtac gggcagcggt cgccccggcc ggcagcgtgt
    23641 actgcaccat gatttcgccc tggtcttcag tgggtaagaa actgcccggc agtttccaca
    23701 tcatcagggc cattccacca cccagtacga cataaagccc catcgttcga gcagcacggc
    23761 gcaggacata aattacgcct cgctgatatt tatcttcagt acggcggtaa aagcggttaa
    23821 atgcgccgaa aaagcctttt ttatgtggcg gaacatgctg gaggacggaa ccgcacaggg
    23881 cgggagtcaa ggtcaatgcc accaccacgg aaagcagcat tgcggagatg atggtgatgg
    23941 agaactgacg ataaattacg cccgtagatc ccccaaagaa ggccatcggc aggaatacgg
    24001 ctgacaacac cagcgcaatg gcaaccagtg cgccagaaat ctcgcccatt gatttttccg
    24061 tggcttcacg cgcgggcaac ccttcgtcac gcataatgcg ttcgacgttc tctaccacca
    24121 caatggcatc gtccaccagc aagccgatcg ccagcaccat tgcaaacaac gtcagggtat
    24181 taatcgaata gccaaacaac gcaagaacgc cgaatgtgcc cagcaaaacg acggggacag
    24241 ctaacgccgg aatcagtgtg gcacgcaggt tttgcaggaa taaatacatc acgcaaacca
    24301 ccaatacgat ggcttcaaac agcgtctgaa ttacgtcctc tacggagatt ttaataaatt
    24361 cagtgctgtc tttcgggtat gcgatgtcgt atccctgcgg catgttccgc tggaattcgg
    24421 caatcttatc cttgaccagc gtcgccgtat tcagcgcatt cgcaccgggc gaaagcatta
    24481 cagcaacccc ggcagacggg tgaccgttaa gtttgccgat agcggtataa tcttcactgc
    24541 ccatctcaac gcgagccaca tcttttatac gaacaactgc gccgtctgac tggcttttca
    24601 cgataatatt tttgaactga tccaccgtct gcaaacgaga ctgcgcacgt accgttgcgg
    24661 tcagttgctg agtattcggt gaaggcaatg cccctatttt cccggcagta acctgcacgt
    24721 tttgcgcttc aatagcactt tgcacgtctg aaggcattag cgagtaagac gccagtttgg
    24781 ccgggtcaag ccagatgcgc atagcgtatt ccgcgccaaa gacttgcaga ctccccacac
    24841 cttcaacacg cgccagcggg tcctgaacgt tactaaccaa ccagtcggcg atatccgaac
    24901 tggaggcttt gtcagtggtg tcatacacgg cagcaatcag caaaaagttg ctttgtgatt
    24961 tctccaccgt aacacccgtt tgctgcactt cgctgggtag gcgcgactcc gcctgctgaa
    25021 ttttattctg cacctgaacc tgtgcagtat ctggatcggt accttgttca aaggtcacat
    25081 taatactgac cgaaccatca gagctgctgg ttgagctgaa atagagtaaa ttatcaagcc
    25141 cagtgagttg ttgctcgata acctgagtca cgctgttctc cagcgtttcg gcagaagcac
    25201 cagtataagt ggctgaaatt ttaatggtcg gtggcgcaac gtcaggatat tgcgcgacgg
    25261 gcaatgtgcg aatagccaga atccccgcca acataatcaa aatggcgata acccaggcaa
    25321 agaccgggcg gcgaacaaaa aagcgcgaaa acatcaggcg ttgcctccat tagcctgaac
    25381 ttcaacagcc ttgacggtct gccctgaagt gactttggca gaaccttcaa caatcagtcg
    25441 gtcgccgttg tgcaggccgt tcagcaccag ccatttatca ccatacgttt ctcccgtttc
    25501 gagcgttcgc tgctctactt tattgtcttt attcaccacc agcgcagttg cattgccttt
    25561 agcatcgcgc gtgacgccct gttgcggcgc gagaatagcg tcttccatca cgccttcatc
    25621 gacacgagcg cgaacaaaca ttcccggtaa taactgctgt tgtggattgg gaaaaatcgc
    25681 ccgtaatgtc accgaaccgg tagactcatc tacagcgact tcggtgagtt ccagacgccc
    25741 tttttcgctg taagttgtgc catcttccag aataagtgag acgctcatgg tgtcactgtt
    25801 ggtcgccagt gactgtttac gcagacgtaa taaatcgaca ctagagcgag tgaggtcgac
    25861 atacattgta tccagaccac gaatagtcgt taacgctgta tcctgcgacg cggtcaccag
    25921 tgcgccaggg gttaccgacg aaataccaat acgccccgaa atcggtgcgg ttaccgtggt
    25981 ccagtcaaga ttaatgcgcg cagtttccag tgcggctttt ttcgcctcta cactggcttt
    26041 atcttgtgca caggtagact gagcatcatc agcatcctgt tgtgaaacac cgttctcttt
    26101 caccagtcgg gcataacgct gcgctttctg gcaatcggct tttaccagtg cctgtgcttg
    26161 ttgtaatgct gcccgagctt cattccatgc agcctgataa ctggccgcat caatctggta
    26221 gagcggctgt ccagccttga ccagatcacc ttccttaaat aagcgtttct ggataattcc
    26281 cccaacctgc ggacgtactt cagcactgag cgcagcactg gtgcgtccgg ttaattcact
    26341 gactaccgaa accggttgag atttgagcgt aacaacgccg acttctgttt ctgacgggga
    26401 tgaattgtta ctttgcgtat tatcacaacc cgataagagc agcattgcca ctacagatgt
    26461 tgctatatac ttcattattt tctccagggt gagaaatacc ctcctcattg aaattaatga
    26521 ggatatgtca gggatattca gttataacga acccgcatcg cgaaatgaat ttcgcaatga
    26581 aataaggggg attaatatcc cccgctattg gttaattctg ttcttattta tttatcaaca
    26641 tactaacaat atcctgatat atttcctgca gatgttgtgg atcgcttttt tgcggtgtta
    26701 aacgacgata aaccgtacct tccatcatgg tagcaaacac ttcaacgcag caacgaatgt
    26761 gctcgtcgct aagatgggga tgcattcttt tcatatgtgc acaaccgtta gcgaacattc
    26821 tggcgtcagc ttcttcaagc attgccataa cccgcgggtt gcgtgtggct tctgcagcca
    26881 cttccagcat cagcgcgtcg tcgtcctcat ttaaggattt acgcaaggca aggacttccg
    26941 ggaagtgatc ggtacgcgcg tcaatgtcca tttgggcaat acgaaagtcg atgatacggc
    27001 gaaccatctc ttcaataatg gcgtctttgt tggcgaagta acgatatatc tgacccacgc
    27061 taagctgagc ttcgctggca atttgcgaca tactggcggc atgaaatccg cagcgacgaa
    27121 aacaccgacg ggcggcgtta atgatttcat cctgacgctg tttgtggcgt atctccagct
    27181 tgctggtcat ttctgtctcc tggcctatca cttgatgaga atgatcgttc tcatttttgc
    27241 taattatacg tttctccaaa gtaatggaaa gtatttttat cttattcatg atgcatattc
    27301 tgataatgaa gagattgtgg agattctgtg aagatagttg acaatattaa gttggggtca
    27361 attcatcaga aagacagcga cttgttgtcg ccagttctgt tttcatcatc aaatttatcg
    27421 gagctaatac gaatagtttc atagtcagat tctataagat atttccccaa cctccccctt
    27481 gcaggcgtca ggagcagata acgcaaaatg ctctgtaccg cagcttctct ccgctacaga
    27541 aagcgcaact atagccaccc acagccaggt tgattataat aaccgtttat ctgttcgtac
    27601 tgtttactaa aacgacgaat cgcctgattt tcaggcacaa caagcatcaa caataaggat
    27661 taaagctatg ggttttcttt ccggtaagcg cattctggta accggtgttg ccagcaaact
    27721 gtccatcgcc tacggtatcg ctcaggcgat gcaccgcgaa ggagctgaac tggcattcac
    27781 ctaccagaac gacaaactga aaggccgcgt agaagaattt gccgctcaat tgggttctga
    27841 catcgttctg cagtgcgatg ttgccgaaga taccagcatc gacaccatgt tcgctgaact
    27901 ggggaaagtt tggccgaaat ttgacggttt cgtacactct attggttttg cacctggcga
    27961 tcagctggat ggtgactatg ttaacgccgt tactcgtgaa ggcttcaaaa ttgcccacga
    28021 catcagctcc tacagcttcg ttgcaatggc aaaagcttgc cgctccatgc tgaatccggg
    28081 ttctgccctg ctgacccttt cctaccttgg cgctgagcgc gctatcccga actacaacgt
    28141 tatgggtctg gcaaaagcgt ctctggaagc gaacgtgcgc tatatggcga acgcgatggg
    28201 tccggaaggt gtgcgtgtta acgccatctc tgcgggtccg atccgtactc tggcggcttc
    28261 cggtatcaaa gacttccgca aaatgctggc tcattgcgaa gccgttaccc cgattcgccg
    28321 taccgttact attgaagatg tgggtaactc cgcggcattc ctgtgctccg atctctctgc
    28381 cggtatctcc ggtgaagtgg tccacgttga cggcggtttc agcatcgctg caatgaacga
    28441 actcgaactg aaataatcgt tctgttggtc aagacgggcg gcgttctgcc gcccgttatc
    28501 tctgttatac ctttctgata tttgttatcg ccgatccgtc tttctcccct tcccgccttg
    28561 cgtcaggata acgatttcct ttacgaccaa ggagcgccca tggaaaaacg ccacattacc
    28621 ggcaaaagct actggtatca tgaaacgcaa tccagtactg cggagtatga cgttctgcct
    28681 ctggtcccgg aagccgcaaa ggtcagcgat ccctttctgc tcgacgtgat ccttgatgaa
    28741 gaaacgctgg cccccttcct ttcatggctg gtccctgcgc gcgttcttgc agtggaattg
    28801 ttccctgacc agcttaccgt gacccgttca cagaccttca ccgcttatga acgcttgtcg
    28861 acggccctga cggttgctca ggtttgcggc gtccaacggt tatgtaacta ctattcggcg
    28921 cgacttacgc cgctccccgg gcctgattcc tccagggaaa gtaatcatcg gttagcacaa
    28981 atcacgcaat atgcccgcca actggctagc tcgccttcta ttatcgacaa ccgatcgcgc
    29041 cagcatctga atgacgtcgg cctaactgcc tgggactgtg taatcattaa ccaaatcatt
    29101 ggttttattg gctttcaggc gcggtcaatt gcgacatttc aggcttatct cgggcatccg
    29161 gtacgctggt tacccggtct ggagatacaa aactatgccg acgcgtcact gtttgctgat
    29221 gaatcaatac gctggcgaag cagctatgag gtggaaaaac tccctgaaga gcacacaaaa
    29281 agttcaactg cagaactttg ccaactggcc gaaacactct ctctccaccc tatttcactt
    29341 tcccttctcg aaaagttgct aaacagcaca cgggtcaata cacagccgga taatcagctt
    29401 gcggcgttgt tatgcgcgcg gataaatggc agtcctgctt gttttgccac ctgtatgaat
    29461 tcatcaaatg aatataaaaa aatcagcgcc cttctgcgca agggcgaaaa tgaaattaac
    29521 caatgggctg accgtcattc tgttgagcgc gctaccgttc aggcgataca atggctgacc
    29581 cgagcacccg atcgctttag cgccgcccag ttcagcccat tactcgaaca cgaaaaatca
    29641 tcaacgcaga ttattaatct gctggtatgg agcgggctgt gtggctggat aaatcgctta
    29701 aaaatcgcgt tgggtgagac atattaacct tgccgcgtca gacagattcg cgtaaaactg
    29761 tcagccgctc taatggccac caaaatagac aattatgttt caggacaacc cgctgctagc
    29821 gcagcttaaa cagcaactgc attcccagac gccacgcgct gaaggggtgg taaaagccac
    29881 agaaaaaggc tttggcttcc tggaagtcga cgcgcaaaaa agttatttca ttccgccgcc
    29941 gcagatgaaa aaagtcatgc atggcgaccg aattatcgcg gttatccaca gtgaaaaaga
    30001 acgtgaatcc gcagagccag aagaactggt tgaaccgttt ctgactcgtt tcgtgggtaa
    30061 agttcagggc aaaaatgacc gtctggccat cgttcctgat catccactct taaaagacgc
    30121 cattccttgc cgcgcagccc gtggcctgaa ccacgagttt aaagaaggcg actgggcggt
    30181 tgccgaaatg cgccgtcatc cgctgaaagg cgatcgttct ttctatgctg aactgacaca
    30241 atacatcact tttggtgacg accactttgt accgtggtgg gttacccttg cgcgccataa
    30301 tctggagaaa gaagcaccag acggcgtcgc taccgaaatg ctcgatgaag gtctggttcg
    30361 tgaagatctg accgcgctgg attttgtcac catcgacagt gccagcacag aagatatgga
    30421 tgacgccctt ttcgctaagg cgttgccgga tgacaaactt cagctgattg tggcgattgc
    30481 cgatccaacc gcgtggattg ctgaaggcag caagctggac aaagccgcga aaattcgcgc
    30541 attcaccaac tatctgcctg gcttcaacat ccctatgctg cctcgcgagc tttctgacga
    30601 tctctgctca ctgcgcacca atgaagtccg cccggtactg gcatgccgca tgacgctctc
    30661 cgctgatggc accattgaag ataatatcga attctttgcc gccaccatcg aatccaaagc
    30721 gaagctggtg tatgacctgg tttctgactg gctggaaaat accggtgact ggaagcctga
    30781 aagtgaagca attgctgaac aagtccgttt gttagcgcaa atttgccaac gccgcggcga
    30841 gtggcgtcat aaccacgcac tggtgtttaa agatcgcccg gattaccgct ttattctcgg
    30901 tgaaaaaggt gaagtgctgg atatcgtcgc cgagcctcgt cgcattgcca accgtatcgt
    30961 cgaagaagcg atgattgccg ctaacatttg tgcggcccgc gtactgcgcg ataagctcgg
    31021 ttttggcatc tataacgtgc atatgggctt tgatccggcg aatgccgacg cgttggcagc
    31081 gttgctgaaa acgcacggtc tgcatgtcga tgctgaagaa gtgctcacgc tggacggttt
    31141 ctgcaaactg cgtcgtgaac tggatgcgca accaactggt ttcctcgaca gccgcattcg
    31201 tcgcttccag tcatttgctg aaattagcac tgaacccggt cctcactttg gcctcggtct
    31261 ggaggcatac gccacctgga cttcgccgat ccgtaaatat ggcgacatga tcaaccaccg
    31321 tctgctgaaa gcggttatca aaggcgaaac tgcgacgcgt ccgcaggatg aaatcactgt
    31381 ccaaatggcc gagcgtcgcc gtctcaaccg aatggcagaa cgtgatgttg gcgactggtt
    31441 atacgcacgc ttcctgaaag acaaagccgg gaccgacacc cgtttcgcgg cggaaattgt
    31501 cgatatcagc cgtggcggca tgcgtgtgcg tttggttgat aacggcgcta tcgcctttat
    31561 tccggcacct ttcttacacg ctgtgcgcga tgaactggtt tgcagccagg aaaacggcac
    31621 tgtacaaatt aaaggtgaaa cggcttacaa agtgactgac gttattgacg tcaccattgc
    31681 cgaagtccgc atggaaaccc gcagcattat tgcgcgcccg gtcgcgtaat ctcctttcac
    31741 ggcccattcc ttatgaatgg gccgtttatt tccccgctct accttcatca tatcccggca
    31801 gttttttaac tgactttcgt ttgaaaactg gcgtttttcc aaaactggat tacaaataaa
    31861 tatataaaaa aaacaatgcg tttagccgcc gttaatttca tgtaagtcat gttgttcctt
    31921 ttatttaacc gcggactccg ctgttaaccg gaggatatgc atcatgaaaa ccgttaggga
    31981 gtccacaacg ttgtacaact ttctcggatc gcacaatcca tactggcggt taacggaaag
    32041 cagcgatgtt ttgcgctttt ctaccaccga aaccacagaa cctgaacgta cgttgcagtt
    32101 atctgccgaa caggctgctc gcatcaggga aatgacggtc atcacctcca gcctgatgat
    32161 gagtctgacc gtcgatgaaa gcgatctttc tgtgcatctg gtaggacgaa aaatcaataa
    32221 acgggaatgg gctggcaacg cgtctgcatg gcatgacaca ccagcagttg ctcgtgattt
    32281 atcacacgga ctttcctttg ctgagcaggt agtttctgaa gcacattccg caatagtgat
    32341 tctcgacagc cgggggaata tccaacgctt caatcggtta tgtgaagatt acacagggtt
    32401 gaaagaacat gacgtcattg ggcaaagcgt gtttaaactg tttatgagcc gtcgtgaagc
    32461 tgcggcatcc aggcgcaata accgtgtatt ttttcgaagc ggcaatgcat atgaagtcga
    32521 actgtggata ccaacacgta aaggccagcg gctgtttctg tttcgcaata aatttgtcca
    32581 cagcggcagt ggcaagaacg aaattttttt aatctgttcc ggcaccgaca ttaccgaaga
    32641 gcgccgcgct caggagcgac tgcgtattct ggcaaatacc gacagtatca ccggactgcc
    32701 gaatcgtaac gcaatgcagg agttaatcga tcacgccatt aatcaggcag ataacaataa
    32761 agttggggtt gtgtatcttg atttggataa ttttaaaaag gtcaacgacg cttatgggca
    32821 tttgtttggt gaccagctat tacgcgacgt gtcattggcc attttaagct gtctcgaaca
    32881 tgatcaggtg ttggcgcgtc caggtggcga tgagtttctg gtactggcat ccaatacctc
    32941 acaaagcgcg ctggaagcaa tggcatcacg aattttgacc cgcttacgac tccccttccg
    33001 cattggttta attgaagttt ataccagctg ttcagtgggt atttcactct ctcccgaaca
    33061 tggttcggac agcgcggcta ttattcgtca cgccgacaca gcaatgtaca cagcgaagga
    33121 aggcggacga ggacaatttt gcgtttttac tccagaaatg aatcagcggg tatttgaata
    33181 tctctggctg gataccaact tgcgtaaagc actggaaaac gatcagttgg ttattcacta
    33241 tcaaccgaaa atcacctggc gtggcgaagt gcgcagtctg gaagcactag tacgttggca
    33301 gtcacctgaa cgtggattga ttccaccgtt ggacttcatt tcctacgccg aagagtcagg
    33361 gctaattgtg cctttaggcc gttgggtgat tctcgatgta gtacgccagg tggcaaagtg
    33421 gcgggataaa ggtattaacc tgcgagtggc ggtaaacatt tctgcacgtc agctcgccga
    33481 tcaaaccatt ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc
    33541 tattgatgtt gaactgacgg agagctgcct gattgagaat gatgagctgg cactgtctgt
    33601 tattcaacaa tttagccaac taggtgcgca agtgcatctg gacgattttg gtaccggtta
    33661 ctcttcactt tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt
    33721 tgttcgagat attcacaaac aacctgtctc gcagtcactg gtccgggcga tcgtcgctgt
    33781 ggcccaggca ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc
    33841 ttttttaacc aagaacggga tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc
    33901 cgccgtcgcc ttcgaacgtt ggtataaacg ctatctgaag cgcgcataag tagatgaata
    33961 tatatactta gcgatatatt ccgtaattat atatcgtatt cccggatgat aattagccgt
    34021 tgctgttaaa cacttctctt ttagcggcaa tttttgcatt attcattttc ccacttttgc
    34081 tataaggatg tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat
    34141 tgtgttagat atccttgtgg caggcgatta ccactctgcg atccataatc tcgagatact
    34201 taaagcagaa ctcttgcgcc aggttgctga atctacgcca gacatcccga aggcaccgtg
    34261 ggagatttaa caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat
    34321 ttttttattt agtattataa cgttataaga attacagcga tgaattcccg acaacaaact
    34381 attctacaga tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact
    34441 ggagtttctg aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc
    34501 cgccgtgcac atggctttgc agtatcgctt gatagtgatg acgtcgaaac ccgtatgatg
    34561 agcaactata cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc
    34621 gaaaccatct ttatcgaaaa tggcagcagc aatgccctgc tcgcccggac tctgggcgag
    34681 cagaagaaaa atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac
    34741 gcgccttgtg aaattatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt
    34801 ggcccgttga cacgccagtg tatccaacag gtacatttca gcaaagcatt tattggtatt
    34861 gatggctggc aacctgaaac tggattcacc ggtcgcgata tgatgcgtac cgatgtggtc
    34921 aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc gaaatttggt
    34981 gctgtacatt catactccat cggtcccgtt gagcgattca atcgcgtgat taccgattcg
    35041 aaaatacgcg ccagcgatct gatgcatctt gagcacagta aactcaccgt tcacgttgtt
    35101 gacatttaac catttccctt ctctacggat gatttgcact ttggcaaatc atccgctcta
    35161 agatgattcc tggttgataa ttaagactat ttacctgtta ttaacactct caagatataa
    35221 aattattatc agcgatataa caggaagtca ttatcacctg cgtgatataa ccctgcgcgc
    35281 gagcggattt cacggaataa tttcaccagg cttattctta gctattatag ttatagagag
    35341 cttacttccg tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat
    35401 gaccgcggct gttctggcaa ttaccctggc aatgtctctg agtgcctgtt ctaactggtc
    35461 taaacgggat cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg gtgcagtact
    35521 gaccgatggc agtacgttgg gtacattagg tggcgcagct gtcggtggtg ttattggtca
    35581 ccaggtcggt aaataatcgt aaataaatgt cgattatcga ctcgcaatta taatacgctt
    35641 gattctgttc agtacgaaat aactgaataa tacacacagt aaaatattcg aaagccacgg
    35701 atatatccgt ggctttttat gttaaccacc ggcgagtttt accttcatcc ctttcgcttc
    35761 aagcagtgat tttaataaat cacgcttatc gccctggatt tcaataattc catctttaac
    35821 tgccccgccg cagccgcatt ttttcttcag ttccgctgcc agttttgtca gttcggcatc
    35881 atcgagatcg acaccggtaa tcaggcaaac gcccttacct ttacgtccac tggtctgacg
    35941 ctgaatacgc accacaccgt cgcctttagg gcgtacaggg gccgcttttg gctcatcaat
    36001 acgtccggtt tccgttgagt agaccagacg gctgttggaa tcactcatca tgcactccgc
    36061 tgtaaagagg cgttgatcgc tttcagcgtt tgcgctggat ctaccgattg cgttaccggg
    36121 cgaccaatca ccatataatc aacaccagcc gccaacgcct gttctggcgt cataatgcgg
    36181 cgctggtcac cagcttcact cccctgcgga cgaatgcccg gcgtaaccag tttgaactcc
    36241 tgaccaaata cctgtttaaa gcgcgcagct tcctgagcag aacacaccac accatcaagg
    36301 ccacattttt gcgtcagtgc cgccagacgt tctgcataat ctgcaggtga cagtgtcacg
    36361 ccaagatcgg ccaggtcgct ggcttccatg ctggtcaata ctgtcacagc aatcaaaagc
    36421 ggtgcatctt tgccaaacgg caccagtgcc tcacgcgctg cggtcatcat acgcgcccca
    36481 ccggaggcgt gaacattcac catccacacg cctaagtcag ctgcagcagc gacagcgtgc
    36541 gctgcagtgt tgggaatatc gtgaaatttc aggtcgagga agatatcaaa accacgctgt
    36601 tgaagttcgc gtacaaattg aggtccaaac agcgtaaaca tctctttgcc gactttcaga
    36661 cgacaatcgc gtgggtcgat cttgtcgaca aaggccagcg cggcatcacg attatgataa
    36721 tcaagggcaa caaccacagg agaattcgta acagcgcggg aagaagatga agcagttaac
    36781 gtcatgacca gaccttcttg atgatgggca ccggaagtgg cgcgaaacag gtaaacggcg
    36841 agcattctac ccgtggaaat aaaaaattaa cagttgcgat ttctcccctg gcaggctttt
    36901 ctgcgccgga gttaccggag ca
//
LOCUS       DAZUCC010000019        36521 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZUCC010000019 DAZUCC010000000
VERSION     DAZUCC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.019"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            410..1783
                     /gene="aroP"
                     /locus_tag="ACHY09_000872"
     CDS             410..1783
                     /gene="aroP"
                     /locus_tag="ACHY09_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFS3883878.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTCFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1826..2680)
                     /gene="ampE"
                     /locus_tag="ACHY09_000873"
     CDS             complement(1826..2680)
                     /gene="ampE"
                     /locus_tag="ACHY09_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFS3883879.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQIDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVLVVIAL
                     LTIYGALV"
     gene            complement(2677..3228)
                     /gene="ampD"
                     /locus_tag="ACHY09_000874"
     CDS             complement(2677..3228)
                     /gene="ampD"
                     /locus_tag="ACHY09_000874"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFS3883880.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLVAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3316..4209
                     /gene="nadC"
                     /locus_tag="ACHY09_000875"
     CDS             3316..4209
                     /gene="nadC"
                     /locus_tag="ACHY09_000875"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFS3883881.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANE
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4412..4852
                     /gene="ppdD"
                     /locus_tag="ACHY09_000876"
     CDS             4412..4852
                     /gene="ppdD"
                     /locus_tag="ACHY09_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFS3883882.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4862..6247
                     /gene="gspE"
                     /locus_tag="ACHY09_000877"
     CDS             4862..6247
                     /gene="gspE"
                     /locus_tag="ACHY09_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFS3883883.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAISFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVENPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAINAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6237..7439
                     /gene="hofC"
                     /locus_tag="ACHY09_000878"
     CDS             6237..7439
                     /gene="hofC"
                     /locus_tag="ACHY09_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFS3883884.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALFPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPVLTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNAGEFSPLCLQLVRTE
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7474..8517)
                     /gene="guaC"
                     /locus_tag="ACHY09_000879"
     CDS             complement(7474..8517)
                     /gene="guaC"
                     /locus_tag="ACHY09_000879"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS3883885.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSFEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(8673..8717)
                     /locus_tag="ACHY09_000880"
     CDS             complement(8673..8717)
                     /locus_tag="ACHY09_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883886.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8742..9362
                     /gene="coaE"
                     /locus_tag="ACHY09_000881"
     CDS             8742..9362
                     /gene="coaE"
                     /locus_tag="ACHY09_000881"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS3883887.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     PREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9362..10105
                     /gene="zapD"
                     /locus_tag="ACHY09_000882"
     CDS             9362..10105
                     /gene="zapD"
                     /locus_tag="ACHY09_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFS3883888.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPVVDYAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            10115..10312
                     /gene="yacG"
                     /locus_tag="ACHY09_000883"
     CDS             10115..10312
                     /gene="yacG"
                     /locus_tag="ACHY09_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFS3883889.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10514..10912)
                     /gene="mutT"
                     /locus_tag="ACHY09_000884"
     CDS             complement(10514..10912)
                     /gene="mutT"
                     /locus_tag="ACHY09_000884"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFS3883890.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHLSLFEILEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVLAKLKRVYVG"
     gene            complement(10972..13677)
                     /gene="secA"
                     /locus_tag="ACHY09_000885"
     CDS             complement(10972..13677)
                     /gene="secA"
                     /locus_tag="ACHY09_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS3883891.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13739..14251)
                     /gene="secM"
                     /locus_tag="ACHY09_000886"
     CDS             complement(13739..14251)
                     /gene="secM"
                     /locus_tag="ACHY09_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFS3883892.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14482..15399)
                     /gene="lpxC"
                     /locus_tag="ACHY09_000887"
     CDS             complement(14482..15399)
                     /gene="lpxC"
                     /locus_tag="ACHY09_000887"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFS3883893.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15500..16651)
                     /gene="ftsZ"
                     /locus_tag="ACHY09_000888"
     CDS             complement(15500..16651)
                     /gene="ftsZ"
                     /locus_tag="ACHY09_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS3883894.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16712..17974)
                     /gene="ftsA"
                     /locus_tag="ACHY09_000889"
     CDS             complement(16712..17974)
                     /gene="ftsA"
                     /locus_tag="ACHY09_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS3883895.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17971..18801)
                     /gene="ftsQ"
                     /locus_tag="ACHY09_000890"
     CDS             complement(17971..18801)
                     /gene="ftsQ"
                     /locus_tag="ACHY09_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFS3883896.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18803..19723)
                     /gene="ddlB"
                     /locus_tag="ACHY09_000891"
     CDS             complement(18803..19723)
                     /gene="ddlB"
                     /locus_tag="ACHY09_000891"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS3883897.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDANPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19716..21191)
                     /gene="murC"
                     /locus_tag="ACHY09_000892"
     CDS             complement(19716..21191)
                     /gene="murC"
                     /locus_tag="ACHY09_000892"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS3883898.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21245..22312)
                     /gene="murG"
                     /locus_tag="ACHY09_000893"
     CDS             complement(21245..22312)
                     /gene="murG"
                     /locus_tag="ACHY09_000893"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS3883899.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22309..23553)
                     /gene="ftsW"
                     /locus_tag="ACHY09_000894"
     CDS             complement(22309..23553)
                     /gene="ftsW"
                     /locus_tag="ACHY09_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFS3883900.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23553..24869)
                     /gene="murD"
                     /locus_tag="ACHY09_000895"
     CDS             complement(23553..24869)
                     /gene="murD"
                     /locus_tag="ACHY09_000895"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS3883901.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAVTGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24872..25954)
                     /gene="mraY"
                     /locus_tag="ACHY09_000896"
     CDS             complement(24872..25954)
                     /gene="mraY"
                     /locus_tag="ACHY09_000896"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS3883902.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25948..27306)
                     /gene="murF"
                     /locus_tag="ACHY09_000897"
     CDS             complement(25948..27306)
                     /gene="murF"
                     /locus_tag="ACHY09_000897"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS3883903.1"
                     /translation="MISVTLSQLTDILNGELKGADITLDAVTTDIRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVNRPLDIDLPQVIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHIQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27303..28790)
                     /gene="murE"
                     /locus_tag="ACHY09_000898"
     CDS             complement(27303..28790)
                     /gene="murE"
                     /locus_tag="ACHY09_000898"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFS3883904.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28777..30543)
                     /gene="ftsI"
                     /locus_tag="ACHY09_000899"
     CDS             complement(28777..30543)
                     /gene="ftsI"
                     /locus_tag="ACHY09_000899"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFS3883905.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30559..30924)
                     /gene="ftsL"
                     /locus_tag="ACHY09_000900"
     CDS             complement(30559..30924)
                     /gene="ftsL"
                     /locus_tag="ACHY09_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS3883906.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30921..31862)
                     /gene="rsmH"
                     /locus_tag="ACHY09_000901"
     CDS             complement(30921..31862)
                     /gene="rsmH"
                     /locus_tag="ACHY09_000901"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS3883907.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31864..32322)
                     /gene="mraZ"
                     /locus_tag="ACHY09_000902"
     CDS             complement(31864..32322)
                     /gene="mraZ"
                     /locus_tag="ACHY09_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS3883908.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32924..33928)
                     /gene="cra"
                     /locus_tag="ACHY09_000903"
     CDS             complement(32924..33928)
                     /gene="cra"
                     /locus_tag="ACHY09_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFS3883909.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(34010..34096)
                     /locus_tag="ACHY09_000904"
     CDS             complement(34010..34096)
                     /locus_tag="ACHY09_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFS3883910.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34108..34599)
                     /gene="ilvN"
                     /locus_tag="ACHY09_000905"
     CDS             complement(34108..34599)
                     /gene="ilvN"
                     /locus_tag="ACHY09_000905"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFS3883911.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34602..36326)
                     /gene="ilvI"
                     /locus_tag="ACHY09_000906"
     CDS             complement(34602..36326)
                     /gene="ilvI"
                     /locus_tag="ACHY09_000906"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFS3883912.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36445..36489
                     /locus_tag="ACHY09_000907"
     CDS             36445..36489
                     /locus_tag="ACHY09_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883913.1"
                     /translation="MKRNDKILHYRGLN"
BASE COUNT         8764 a        10215 c         8947 g         8595 t
ORIGIN      
        1 gctcgcaata ttatatttaa atactcgcgt tacaaaccca ttgtattatt aacatctatt
       61 tattcttata tgaccttttg taagattatt gtgctggtgg ctaaggcata gttttcagca
      121 ggcagacaaa tccggcatat agctagttaa aacagtatgt tatcagactt aaagtatcta
      181 ttaaatgtga tgaaaaactt acaattgctt tttttttatc caaaaaatca acgcatctac
      241 ataaagcagg tgcattcccg ggcacatacc attatcttgt gatagttaaa agtagcatta
      301 tcataattca gttacatgaa acgtaaagaa atcaatacaa caaacggaat tgcaaactta
      361 cacacgcatc actgcgtaga tcaaaaaaac aatcaccgca cgaggtttca tgatggaagg
      421 tcaacagcac ggcgagcagc taaagcgcgg tcttaaaaac cgccatattc agcttatcgc
      481 gctgggtggc gcgataggga ccgggctatt cctgggtagc gcctccgtaa tacagtccgc
      541 agggccaggg attatcctgg gttacgccat tgctggtttt atcgcctttc tgatcatgcg
      601 tcagctaggt gaaatggtgg tcgaagaacc tgtcgcaggc tcctttagcc actttgctta
      661 taaatactgg ggcagttttg ccggtttcgc ctctggctgg aactactggg tactgtacgt
      721 tttagttgcc atggctgagc tgactgccgt gggtaaatac attcagttct ggtatccgga
      781 aatcccaacc tgggtttctg ccgccgtgtt ctttgtggtg attaacgcca tcaacctgac
      841 caacgttaaa gtgtttggcg agatggagtt ctggtttgcc attatcaaag ttatcgcggt
      901 ggtagcgatg atcatcttcg gcggctggct gctattcagc ggcaacggcg gcccgcaggc
      961 gagcgttagc aacctgtggg atcagggtgg tttcctgccg cacggcttca ccgggctggt
     1021 gatgatgatg gcgattatca tgttctcttt cggcggtctg gaactggtgg ggatcaccgc
     1081 agcagaagct gataatccgg agcaaagtat cccgaaagca actaaccagg ttatctaccg
     1141 catcctgatt ttctatattg gttcgttagc cgttctgctc tcgctgatgc catggacccg
     1201 cgttactgct gataccagtc cgtttgtact gatcttccac gaattgggcg atacctttgt
     1261 ggctaatgcg ctgaacatcg tggtactgac tgctgcgctc tccgtgtaca acagctgcgt
     1321 atattgcaac agccgtatgc tgtttggtct ggcacaacag ggcaacgcgc caaaagcgct
     1381 ggcgtctgtc gacaaacgcg gtgtaccggt taacactatt ctggtgtctg cgctggttac
     1441 ggcattgtgc gtgctgatta actaccttgc accggaatcc gcattcggac tgttaatggc
     1501 actggtggta tctgcactgg taatcaactg ggcgatgatt agcctggcgc atatgaaatt
     1561 ccgtcgcgcc aagcaggaac aaggcgtggt aacttgcttc cctgctctgc tttatccgct
     1621 gggtaactgg atctgcctgc tgtttatggc ggcggtactg gtgattatgc tgatgacccc
     1681 aggaatggcg atttcggtat acctgatccc ggtatggctg atcgtgttag gtatcggcta
     1741 tctgtttaaa gagaaaaccg ccaaagccgt aaaagcgcat taatctctct acgccctcac
     1801 ccgtgcaggg tgagggcaat aatctttaca ccaacgcccc gtaaatcgtc agtagcgcaa
     1861 ttaccaccag gaccacgaac gaggttttct tcgccatgga aaccgctgcc ttcggcgtct
     1921 ccaccttatc gacatgcggt tcacgcgcca gagagaactg tgccagacgt gttaatacct
     1981 gatattgcga agtatggaaa tcacccagcg aagcaaacca ggccggtaac gctttctccc
     2041 catgaccgat caaggcatat accacacccg caagacgaac cggcacccaa tccagtacat
     2101 gaagcacggc atcaatgccg gactgtaaac gatgatgcgg cgtctggtaa cgcgccagcc
     2161 agtattgcca tgcacgcaaa aacgcatacc ccatcagcgt aacgggtccc caggttcccc
     2221 ccacaatcag ccagaacagc ggtgcaagat aaaaacgaaa gttaatccac agcaatgcat
     2281 tttgcagctc acgcaaatac tcacgttcat cgcagcctgc cgggacgccg tgaatcatgg
     2341 tgagttcgcc agccatcgtg gcacgggcat ggctatcatt acgtgaagca gctgtcagat
     2401 aagcatgata atgaagacga actttacctg cgccaataca cagcaaacca atcagcagcc
     2461 acaccagcag cgtgggaacg ttgaacaata ctccctgcaa tgcgcgtaac agtaaaaaag
     2521 tcacgcccat cgcaataatg gtcatgccta atgtgcgccc gagagaaaaa tgtttcaccc
     2581 gccgaaagaa cgcttcaaga cgatgatcaa tctgccagtg ctcgcccaac ttaaacaggc
     2641 gctcgaaaat taacaccagt aaggttgtaa atagcgtcat gttgtctcct tgctgaccag
     2701 tgcacgaaac cgtgcccaat caaatgcagg gccgggatcg gttttccgat ccggcgcaat
     2761 atcacaatgg cccgtcatgt tattagcgat atctggatag cgatcaatca gtgcccgcgt
     2821 aaccgccaca agctgttgat actgcgcatc ggtatacgcc agcgtatcgg tgccttccag
     2881 ctcaatccca atagaaaaat cattgcagcg ttcgcgcccc tgatactgag agactccggc
     2941 atgccaggca cgtttatcga aaggaacata ctggactatt tcaccatcgc ggcgaatcaa
     3001 acagtgagcg gagacgcgca aatgggcgat ctcagcaaag aaaggatgcg cctgcggatc
     3061 aatagttcca gtgaataatg cgtcgatcca cggaccgcca aactcgcctg gcggcaggct
     3121 aatattgtgc accaccagca gggtgggtgt ttcgtcatcc gggcggcaat cgtaatgtgg
     3181 tgagggaacg cggcgcgcgc caaccagcca cccctgttct aacaacatgc aggatctcct
     3241 tatatgtggt gctaataccc ggttcagagt agcatgtttc taccttatga ttcgttagct
     3301 atctggagtt ttaacatgcc gcctcgccgc tataaccctg acacccgacg tgacgagctg
     3361 ctggaacgca ttaatctcga tatccccggc gcggtggccc aggcgctgcg ggaagattta
     3421 ggcggaacag tcgatgccaa caatgacatt acggcaaaac ttttaccgga aaactctcgc
     3481 tctcatgcca cagtgatcac acgcgagaat ggcgtatttt gcggcaaacg ctgggttgaa
     3541 gaggtgttta ttcaactggc aggcgacgat gtcaccataa tctggcatgt ggatgacggc
     3601 gatgtcatca acgccaatga acccttgttc gaacttgaag gcccatcccg cgtgctgtta
     3661 acgggcgaac gcactgcact taattttgtg caaacccttt caggggttgc cagtaaggta
     3721 cgccactatg tcgaattact ggagggcacc aacacgcagt tgttggatac gcgcaaaacc
     3781 ttacccggcc tgcgttcagc tctgaaatac gcggtacttt gcggcggcgg agcgaatcac
     3841 cgcctggggc tttctgatgc cttcctgatc aaagaaaacc atattattgc ctccggctca
     3901 gtgcgccagg cggtcgaaaa agcgtcctgg ctgcacccag atgcgccagt agaagtcgaa
     3961 gtagagaatc tggaagaact tgatgaagcc ctgaaagcag gtgccgatat catcatgctg
     4021 gataacttcg aaacagaaca gatgcgcgaa gccgtcaaac gcaccaacgg caaggcgcta
     4081 ctggaagtgt ctggcaacgt cactgacaaa acactgcgcg aatttgccga aacgggcgtg
     4141 gactttatct ccgtcggtgc gctaactaaa cacgtgcagg cactcgacct ttcaatgcgt
     4201 tttcgctgat tgctgacaga tttatccgct ccatcgcggg cggataatct cccctttccc
     4261 aactttcttc gtaacgcctc gcaaatttct tgtctttcgc tgcaacgtaa taaaaaactc
     4321 cggaagctgc tttcccgttt cgttttttgc ccctcgcttg tgctgatggc gtttgccaaa
     4381 gtagcgccaa ccaaatcaag gagcgaaaca aatggacaag caacgcggtt ttacacttat
     4441 cgaactgatg gtggttattg gcatcattgc cattttaagc gccattggta ttcccgctta
     4501 tcaaaactac ctgcgcaaag ccgcactcac cgacatgcta caaacctttg tgccttaccg
     4561 taccgccgta gagttgtgcg cgctggaaca tggtggatta gatacctgcg acggtggcag
     4621 caatggcatt ccctcgccta ccaccacccg ctatgtttca gccatgagtg tggcaaaggg
     4681 cgtggtgtcg ctgaccgggc aagaaagtct caatgggcta agcgtcgtca tgacaccggg
     4741 ttgggataac gcaaacggcg tcaccggctg gacgcgcaac tgcaatattc aaagtgacag
     4801 cgcattgcag caagcctgcg aagatgtctt tcgctttgat gacgccaact aaggagcggc
     4861 aatgaatatt ccacagctca ccgccctgtg tctgcgttat cagggagtct tgctggatgc
     4921 cagcgaagag gtggttcatg ttgcggtggt cgatgcccct tcgcatgagc tactggacgc
     4981 attgcatttc gctaccacca aacgtattga gatcacctgc tggacgcgcc aacaaatgga
     5041 aggtcacgcc agtcgcaccc aacagacatt gcccgtagct attcatgaga agcatcagcc
     5101 caaagcagag ttgctgactc gaacgttaca atctgcgctg gaacaacgcg cgtctgatat
     5161 tcatatcgaa ccagcggaca atgcctaccg catccgcttg cgtatcgacg gtgtattgca
     5221 tcctttaccg gatgttttac cggatgccgg agtcgcatta accgccagat taaaagtgct
     5281 gggcaacctg gatattgcgg aacatcgcct gccgcaggac gggcaattca ctgtcgaact
     5341 ggcaggaaac gccatctcat ttcgtattgc gaccttacca tgtcggggtg gtgaaaaggt
     5401 ggtattaagg ttgttacagc aggtgagtca ggcactggat gtcaacacgc ttggaatgca
     5461 gccgttacaa ctggcggact ttgctcatgc cttgcaacaa ccacagggac tggtgctggt
     5521 aacaggccct acaggcagcg gcaaaacggt cacgctttat agtgccctgc aaacgctgaa
     5581 taccgctgac attaatattt gtagcgtcga aaatccggtt gagatcccca tagccggact
     5641 aaaccagacg caaatccatc cgcgtgccgg actcaccttt cagggcgttt tacgtgcgtt
     5701 attgcgccag gatcctgacg tcattatgat cggagagatc cgcgatggcg aaacggcaga
     5761 aattgccatt aacgccgcac aaacgggtca tctggtattg tccaccctac acaccaactc
     5821 cacctgcgaa acgctggtac gtttacagca aatgggagtc gcccgctgga tgctctcatc
     5881 agcgcttacg ctggtaatag cccagcgtct ggtacgcaaa ctttgcccac attgtcgccg
     5941 acagcaaggg gagccaatcc atattccaga caatgtatgg ccgtcgccgc tgccccgctg
     6001 gcaagcaccc ggttgtgtac attgctacca cggtttttat ggtcgcacgg ccttatttga
     6061 agttctgccc ataacaccgg tcatacgtca gcttatttcc gctaataccg acgttgaatc
     6121 gctggaaacg cacgcccgac aggcgggtat gcgaacgctt tttgaaaacg gctgtctggc
     6181 cgtggagcaa ggcttaacca cctttgaaga gttaatccgc gtattgggga tgccgcatgg
     6241 cgagtaagca actctggcgc tggcgtggca taaccggcga cggcaatgcg caagatggga
     6301 tgctatgggc agagagccgt gctttgctgc ttatggcact acagcaacag atggttaccc
     6361 cactaagcct gaagcgaatc gccatcaatt ctgcgcagtg gcgaggagat aaaagcgcgg
     6421 aagtcattca tcaactggcg acgctactca aagccgggtt aacgctttct gaagggctgg
     6481 cactgctggc ggaacagcat cccagtaagc aatggcaagc gttgctgcaa tcgctggcgc
     6541 acgatcttga acagggcgtt gccttttcca atgccttatt tccctggtca gaggtatttc
     6601 cgccgctcta tcaggcgatg atccgcacgg gtgaactgac cggtaagctg gatgaatgct
     6661 gctttgaact ggcgcgtcag caaaaagccc agcgtcagtt gaccgacaaa gtgaaatcag
     6721 cgttacgtta tcccattatc attttagcga tggcaatcat ggtggttgtg gcaatgctgc
     6781 attttgtgct gccggagttt gctgctattt ataagacctt caacacccca ctaccggtac
     6841 taacgcaggg gatcatgacg ctggcagact ttagtggcga atggggctgg ctgctggtgt
     6901 tgttcggatt tctgctggcg atagccaata agttgctgat gcgccgcccg acctggctta
     6961 tcgcgaggca gaaattgctg ttacgcatcc cgattatggg ttcactgatg cggggacaaa
     7021 aactcacgca gatctttacg attctggcgc tgactcaaag tgcaggcatt acttttttac
     7081 agggcgtaga gagcgtcaga gaaacaatgc gctgcccgta ctgggtgcaa cttctgacac
     7141 aaatccagca cgatatcagt aacggtcaac ccatctggct ggcgctaaaa aatgccggtg
     7201 agtttagccc actctgtttg caattagtga gaacagaaga ggcatccggc tcgctggatc
     7261 tcatgttaga caacctcgcc catcatcatc gggataacac aatggcgctg gcggataacc
     7321 tcgcagcctt actggaaccg gcgttgctga tcataacggg aggaattatc ggtacgctgg
     7381 tggtggcaat gtatctgcca attttccatt taggcgatgc gatgagtgga atgggataac
     7441 gctggcgtgt tgctccacgc cagcgttggg agattacagg ttgttgaaga tgcggttttc
     7501 ttgttccagc acacggataa atgtggtgcg cttggtcagc tctttcaggc gtgaagcccc
     7561 aacgtatgta caagctgaac gcaggccgcc caaaatatca cgcgcggtat tttcaaccgg
     7621 gcctcgcagc ggcagcttaa cggttttacc ttctgctgcg cgatattccg caacgccacc
     7681 aacgtgacgt ttcatcgcag actcggagct catgccgtag aacagcataa atttctcgcc
     7741 gttctcctca acgatgcgac cgccgctctc ttcgtggccc gccagcatgc cgccaagcat
     7801 gacgaaatcg gcaccgccgc caaaggcttt cgcgacatcg cccggcgtgg tgcagccacc
     7861 gtcgctgacg atcattccgc ccagaccgtg cgcagcatcg gcacattcga tcaccgcaga
     7921 aagttgcgga taaccgacgc ctgttttgac gcgagttgta caaacagaac ctgggccaat
     7981 gccaacttta acgatatcag caccggagag gataagctcc tcacacattt caccagtcac
     8041 tacgttgcca gcacaaatgg ttttggtcgg ccacgcttca cgcgctttcg caacgaactg
     8101 cacgaagtgt tcggaataac cattcgccac gtcaatacaa acgaagttta atgccgggtt
     8161 caggtcgaga atctgtttag ttttttcgaa atccgcatca gacgtgccgg tagaaaccat
     8221 cacatgtttc agcacatcag cggaagaatt gttgataaac gcttgccact cttcgaaaga
     8281 atagtgttta tgcacagcag tcaaaatatc aaaagaagcc agcgcagagg ccatagaaaa
     8341 tgtgcctacg gtgtccatat ttgcggcgat aatcggcacg ccggaccagc tctgacctga
     8401 atgtttgaag gtgaattgac gttccagttc aacatcggaa cggcttttaa gagtggagcg
     8461 tttagggcgg atgagaacgt ctttaaaacc taacttcaga tcttcttcaa tacgcatgtg
     8521 cggattcctg gggttaatgg ctataagggt aagagcgcaa ctccagtgac gctatcatac
     8581 gcactaatca atgcggcgca agactgcgaa attgtctttt tttacgctac aatcccataa
     8641 atttacctgg taaatagtga gcgaacgttt catcaatttc gctttcgtgc gctcattttt
     8701 aacgttttga taatcatgat gaattcccgg gaatataaat tatgaggtat atagttgcct
     8761 taacgggagg cattggcagt ggcaagagta ccgttgccaa tgcgtttgct gatctcggaa
     8821 ttaacgtcat tgatgccgat attattgcgc gtcaggtggt tgaaccaggt gcacctgcgc
     8881 tacatgccat tgctgatcac tttggcgcta acatgattgc tgctgatgga acattgcagc
     8941 gccgggcctt gcgcgagcgg atcttcgcca acccggaaga gaaaaactgg cttaacgccc
     9001 tgctgcatcc gctgattcag caagagacgc aacaccagat ccagcaagca acctccccct
     9061 atgtactgtg ggttgtgcca ttgctggtag aaaactcact gtataaaaaa gcgaatcgag
     9121 tgctggtggt ggatgtcagc ccagaaacgc aacttaagcg caccatgcag cgcgatgatg
     9181 taactcgcga gcatgtcgaa caaatccttg ctgctcaggc accgcgcgaa gctcgccttg
     9241 ccgtggcaga tgacgtcatt gataataacg gcgcaccgga tgctatcgca tcggatgttg
     9301 cccgcctgca cgcacactat ttgcagcttg cgtcgcagtt tgtctcacag gaaaaaccgt
     9361 aatgcagacc caggtccttt ttgaacatcc actaaatgaa aaaatgcgta catggctgcg
     9421 cattgagttt ttaattcaac aactcaccgt aaatttgccc gtcgttgact acgctggcgc
     9481 tctgcatttc ttccgcaatg tcagtgaatt gctggatgtt ttcgagcgcg gcgaagtccg
     9541 cactgagttg ttgaaagaac ttgaccggca gcaacgtaaa ctccagacct ggattggcgt
     9601 gcctggcgtg gaccagagcc gtattgaagc attaattcag cagttaaaag cggcggggag
     9661 cgtattaatt tccgcgcccc gtatcgggca atttctgcgt gaagatcgtt tgattgccct
     9721 ggtgcgtcag cgactgagca tccccggcgg ctgttgtagc tttgatttac ctacattgca
     9781 catttggctg catctacccc aggcgcagcg cgacagccag gtagaaacct ggattgccag
     9841 cctgaacccg ctcacccagg cacttaccat ggtgctggat ttaattcgcc agtcggcccc
     9901 cttccgtaaa caaaccagcc tgaatggttt ttatcaggat aacggtggcg atgccgactt
     9961 gctgcgcctg aatctgtcac tcgattcaca gctttatccg caaatttccg gtcataagag
    10021 ccgttttgcc attcgtttta tgccgctgga cagtgaaaac ggacaggtac cggaacgtct
    10081 ggatttcgaa ctggcctgtt gctaaggagt aaaaatgtca gaaactatta cggtgaattg
    10141 cccaacctgc gggaaaacgg tggtgtgggg tgaaatcagc ccgtttcggc cattttgctc
    10201 caaacgttgt cagctgatcg acctcggaga atgggctgct gaagaaaaac gaatccccag
    10261 cagtggcgat ctttccgaaa gcgatgactg gagcgaagag ccaaagcagt gacattttat
    10321 ctgatgcgac gctggcctga taagacgcgg gcagcgtcgc atcaggcatc gtgtgcaaat
    10381 gtcggatgcg gcgggaacgc cttatccgac ctacaagacc ttacatatca acatgttaaa
    10441 attgtagata ctgataagac gcgccagcgt cgcatcgggc atcttgtgca aatgttggat
    10501 gcggcataaa cgcctatccg acatacacac gcttaagctt cgcaagtacc ggttcattgg
    10561 ctggcggaaa atcatcggca ttcagaccga ccagcgacat ccactcaccg ggttgccctt
    10621 ctttacccca cggctcccct tcccagcttt cgaccagcca gaaccacagt gttatatgcc
    10681 tgtccgggaa ttcatattcc agtatttcaa atagcgaaag atgttgtggg gtaatcccga
    10741 cttcttcctg aagttcacgc accaccgcct gttccggcgt ttcacccatt tcaattttac
    10801 cgccgggaaa ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgg gttataaaga
    10861 tttcattgtt ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa
    10921 acctataaaa aaggcgcaga atcctgcgcc ttttacttca actgttagct tttattgcag
    10981 gcggccatgg cactgcttat attttttacc agaaccacac gggcaaggat cgttacgtcc
    11041 tactttgcgt tcaccggttt gcgccgccag tgcagctgcg gcagcagagt cgtcatcctg
    11101 atggctaagc tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag
    11161 ctcctcaacc tcttcaggca tacgtacctg aactttgctc agcgtgctga taacttcata
    11221 ttttaacgac tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt
    11281 cggatctttc tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgcagc
    11341 caggtgctct ttccacagag agtcaagcgt ttgcagcatg acgcctttct cgaagtgacg
    11401 catcatctca gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag
    11461 aatgcgctca cgcagcgtct cttcatgcag ttctggttct ttatccagcc actcggcaat
    11521 tggcaaatcg aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat
    11581 ttcttccagc gactgtggcg gaatgtaggc atcaatggtc gctttgaaca catcttcacg
    11641 aatgctgttg atggtttcgc tcacatcgct gacatccagc agttcgttac gctgggagta
    11701 aatggcgcga cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc
    11761 gaagttacgg ctttcaactt tacgctgggc gttggcaatc gctttggtca cccacgggtg
    11821 ctcaatggct tcgcctggct tcatacccag tttacgcatc atgccggata ctcggtcgga
    11881 agcaaaaata cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc
    11941 cccctgacga ccagaacgac cgcgcagctg gttatcgata cgacgggatt cgtgacgttc
    12001 agtaccgatg atatgcaggc cacctgctgc cagtaccgca tcgtgacgta cctgccagtc
    12061 ggctttaatt ttttcaattt gctctgcagt cggattttcc agcgcggcaa cttctgcctg
    12121 ccagctacca ccgagcacaa tatcggtacc acgacccgcc atgttggtcg cgatagtcac
    12181 cgcagccggg taacctgcct gagcaacaat cgccgcttcg ttggcatgga atttggcgtt
    12241 caggacgttg tgcttaatac cggctttggt cagttcattt gacaccagct ccgatttttc
    12301 gatggagatt gtacccacca gcaccggctg gcctttcgca gtgcgttctt tgatatcttc
    12361 aatgatcgcc tgaatttttt ccgcttcagt catgtagacc aggtccggca gatctttacg
    12421 aatcattgga cggttggtcg gaacaacgac agtatccagc ttatagatgg agctgaattc
    12481 gaaagcttcg gtatcagcag taccagtcat ccccgccagt ttttcataca gacggaagta
    12541 gttctggaag gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt
    12601 cgcttccaca gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt
    12661 gtgttcgtca acgatgataa cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa
    12721 cagcgcatga gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt tggccggaga
    12781 gtacagagac tcaccttcat ccatgatgcc ttctttaacc agcagttctt caatcagaac
    12841 cagaccacgt tcggtcaggt tcacctggcg agatttttca tccaccgaga agtggccttc
    12901 gccctggaag gtttcggagt cttctttttc ctgacggatc aggtgtggaa taattttatt
    12961 cacgcgttta tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg
    13021 cgcttcatcg atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg
    13081 tacacgttct tcaggactga acgccatgtt gtcgcgcagg tagtcaaagc cgtattcgtt
    13141 gttcgtaccg taagtaatgt cagctgcgta agcttcacgc tttgccggtg ctggcatgcc
    13201 cggcaggttg ataccaactg tcaggccaag aaattcaaac agcggacggt tgttttcggc
    13261 gtcacgttgc gccaggtagt cgttgacggt aactacgtgc acgcctttac cggttagtgc
    13321 gttcaggtaa gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc
    13381 gatgcagcgt tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc
    13441 aaagacgcgc ttacttgcct cacgcaccac ggcgaaagct tccgggatca gattttccag
    13501 cacttcgcct ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc
    13561 ggagagtttt tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg
    13621 gcgcagggtg cgatcgttac gactaccgaa aactttagtt aataatttga ttagcataat
    13681 aaaatctcaa acgccccgcg ttgcggagtt aataaaatga agtaacggtt tattgttgtt
    13741 agctgaggcg ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc
    13801 tgaattttgc ttgcggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg
    13861 tgccttcctg ggtcaacagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa
    13921 gaggcaaaga ttcttcagca acgggcagcg tttgcggtgc cattgcgaag gaaagatgac
    13981 ggattaccgt gcgaatggca tgttgatgcc agtaatcaac ggaatagttc gaattcgggc
    14041 gacgcgtgtt cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt
    14101 ggttgcgggt tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca
    14161 aacctaaact cgccgcgacc atccctaata agagatgcgg ccagaagtag cgtttaccaa
    14221 actgtcgcca gcgcgtcagt attccactca cgttattgcc atcccgatcg tattcaaacg
    14281 tatttagcgc acaaatctta tcattaaagc ctgaagtcca cagcacgaat gaagataagt
    14341 tgtgcgtaaa agtgcttaag aaaacagcgt tcgcaccgtt ttttatcgtt cttataaaat
    14401 tcaaaaaaaa acgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt
    14461 tatacgacag tataaatgtc gttatgccag tacggctgaa ggcgctttga aggccaacgg
    14521 cagttctgcg tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc
    14581 ctgcagcagt ttgttattca gtgcatgacc agatttataa gcggtaaatg caccaataat
    14641 attgtgacca cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc
    14701 gtcttcaaaa cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca
    14761 atcgaagctg ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa
    14821 accgaacgta cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc
    14881 atagcgctgg ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga
    14941 aaaaccattg tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc
    15001 tttgatgcga acaaatttct tggcgcagtt cagctcgtcg ataccagcgt caagcagcag
    15061 gtatacaaac ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat
    15121 aacaatgtta tcgatgccca ggcccgcgag agcagcattg aggtgctcta cggttgaaat
    15181 ccgtacatca tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc
    15241 atcggccggg aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt
    15301 ggccggcgca gggcgtaacg tcagggtgac tttcttgccg gtatgtaaac cgacacccgt
    15361 cgcctgaacg atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac
    15421 ctatccaacc gaagtgtact atacattcgg cgggccagtt tagcacaaag agcctcgaaa
    15481 cccaaattcc agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat
    15541 aatccggctc tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct
    15601 gctcctgggt cagcggagcc atcccgtgct gctggtagcg atccatcact ggctgctgaa
    15661 cctgcttatt ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa
    15721 caacagttac gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag
    15781 tcgcgttgtc ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac
    15841 gcaggtcgaa gcccgctgtg atgtttacca gtacgccgcg tgcgccagac aggtcgatat
    15901 cttccagcag cggagaagag atagccattt cagcagcttc ttccgcacga tcttcaccgc
    15961 tcgccacacc agaacccatc attgcgtagc ccatttcaga cattacggtg cgtacgtctg
    16021 caaagtccac gttcatcaaa cccggacgag taatcagttc ggcgatacct tgcacagcgc
    16081 ctttgagtac gtcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa
    16141 ctttcagcag tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag
    16201 tgatcccctg ctccgcgaat gccatacgct tcttgccttc aaagttgaag ggcttagtga
    16261 cgacagcaac ggtcaggata cccaaatctt ttgccacttc agcgacgact ggagctgcgc
    16321 ctgtaccggt accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg
    16381 ccgcacgcag tgcatcgcga tcctcatcag ccgcattgcg gccaacttct ggattagcgc
    16441 cagctcccag tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt
    16501 tacgcagcgc ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atgcgctcgc
    16561 gcaccatgtg ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg
    16621 cgtcattggt aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct
    16681 gagaccgtaa tcattgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga
    16741 gtcgcttgat ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt
    16801 taagatgtga ctctttcccg taatgaagca atcccaccgc cgtcgaataa tacggctcct
    16861 gagcataatc cgttaaaccg gtaatgttca gcggcgcgcc gatacgcacc tgcgtatgaa
    16921 acacgcgctg agcacaggct gcaagacctt caatctgcgc tgcgccaccg gttaatacaa
    16981 tgcctgccgc caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata
    17041 tctcttcgtt gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg
    17101 tctgacgttg cagactccgc ggcggacgac cgcctacgct cggcacttcc acgctctcat
    17161 cttttccaac gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc
    17221 ttggcggcgt gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa
    17281 ttaccttagt gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac
    17341 cgatatcgac gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg
    17401 atgccagtcc ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga
    17461 cgatgttttt cgccatatcg ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca
    17521 cgcccgaaag tcctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg
    17581 ggatcacatg cagcacacgg tgttcatcgc gcacacgcac cgatttcgcg gtatggacaa
    17641 cgttttccac atcttcttgc gtcacttctt cttcagaaat aggcaccata ccaatttcat
    17701 tctggcagct gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg
    17761 ccatcaattc tgcctggtca atggcgcgct gtacgcactt gaccacggat tcgaggtcgt
    17821 tcaccccgcc tttatccata ccacgcgacg ggcagctgcc cacgccaatg atattgacca
    17881 taccgtcggg cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc
    17941 ctactaccag ttttctgtcc gtcgccttaa tcattgttgt tctgcctgtg cctgattttg
    18001 ttgctgagta gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata
    18061 acgcaaatca acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg
    18121 ataaagttct acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt
    18181 aatatcgtta ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag
    18241 agtaaatctg tcctttgcca gcatctgccc catttcgcga tagccctgca acacttcatt
    18301 ggcgctgcct tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg
    18361 cacgctgaaa gtgtttcctt ccgcgtctac catatgttga tcattccacc gcgcaatcgg
    18421 cacatattca accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg
    18481 cttaatccac ggcaggcgtt gttctatttg cgtctggatg atgttgacat cctgggtcat
    18541 aaaggtacct ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta
    18601 atggcgttca ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca
    18661 gcccaacacg acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat
    18721 ccccgccaga cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tgtttcgcgt
    18781 gttcagagca gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg
    18841 agaagctcat acctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg
    18901 gtgaggtatt ggcttccagc agataaaact gtccatcgct gtccagcata acgtcaatac
    18961 gtccccatcc tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg
    19021 cctcttgtga cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact
    19081 tcgcctcata atcatagaag gttccggacg gttgaatacg tattgacggt aaaatttctt
    19141 caccgagtat cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt
    19201 catcgtgctg aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg
    19261 acattcccac actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag
    19321 aaatttctgc taactgctta tcactcaggc ctttttcaaa ctctgcgcgg gttaacgcta
    19381 cccacggagc gaccggtaaa ccggcacctt gccacagaag tttgctgcgt agtttatcca
    19441 ttgaaagcgc agatgccatc actccgctgc cggtataagg caagcccatc agctcgagca
    19501 tgccctgcag cgtgccatct tcaccgccgc gaccgtgtag cgcgataaac actttctgaa
    19561 agcccatcga cttcagttgc gtcacgtcga cttctttcgg gtcgacagga ttcgcgtcaa
    19621 taccgccttc acgcagcccg gctaacaccg ctgcgccaga attcagagaa acttcccgct
    19681 cagcggaggt cccacccaac aggaccgcga ttttatcagt catgttgttc ttcctccgga
    19741 gtttgcggct tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc
    19801 ccctgaacga gaatcaggtc gttaccggtt aataccggag ccagcatctc ggctacctgc
    19861 gccgggtccg gcaccagaat gggatcaatt ttcccacgtc cacgaattgt gcgacacagc
    19921 gaacggctgt ccgctcccgg aattggcgct tcgccagccg gatacacttc cagcatcaac
    19981 agggtatcaa cctgcgtcag cacattggcg aaatcatcat acaggtcgcg cgtacgggta
    20041 aaacggtgcg gctgaaacag cattaccagg tttttatccg gccagcctgc acgcgccgct
    20101 ttaatggtgg cgtccacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta
    20161 ccgcttttac cattcacagg ctccagcggg aattcaccga ggaaatcaaa acggcgacca
    20221 gtcccctgga agctctccag cgcacgcaaa atagcctcgt cgtcaattcc ctcttccgta
    20281 gcaaccgcaa ccgcagctgc ggcgttcagc gcgttatgac ggcctggcgc attcagggtg
    20341 acgcgcatcg gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc
    20401 tgataatctt ctacacgcac gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt
    20461 cccacacgcg gtaataattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg
    20521 taaaacggca ggttgtgcag aaaattaata aaggtctgtt ttaaattctc aaagtcgccc
    20581 tggtaggtat ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga
    20641 tgcaggaacg atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa
    20701 cgcgcatgaa cgcccgccgc ttttaccagc ccaccgttaa cgaaggttgg gtcgagtccc
    20761 gcttctgcgt agatgctgga aaccatcgca gtggttgtcg ttttgccgtg cgttccggca
    20821 atggcgatgc catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc
    20881 ggaatacgcg cttcatgagc tgcgacaatt tccgggttat cggcagaaat cgcgctggaa
    20941 acaacgacca cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgcc
    21001 ccaagattca ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga
    21061 taaccttcat tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca
    21121 aaatgtatgt gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt
    21181 tgtgtattca ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct
    21241 acaattacgc ccgggcagcc cggctcactt catttgccac tcgctcggtg gcatccggaa
    21301 tggatgcggc gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca
    21361 gggtgttggc gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg
    21421 ctttttccag cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa
    21481 acaacgccgg tagtcctgcc gcggcgattt cactcaccgt taacgcaccg gagcggcaaa
    21541 cgacgacatc cgcccacgca tacgccgccg ccatatcatc aataaattcc gtcactttat
    21601 gctgcggttg ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc
    21661 tctgatgcca gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt
    21721 taagaatgcg tgcgccctgg gaaccaccca ctaccaacac acgaaccgga ccttcacgtc
    21781 cagccaaacg ttgctgcggc aacggcagcg ccagcacatc ggtacgcacc gggttaccca
    21841 ccacttccgc attagggaaa gcgcctggaa acgcctgcat cactttggtg gcaatcttcg
    21901 ccagccattt attggttaat cccgcaatac cgttttgttc atgaagcaca accggaatac
    21961 ctaacgacca cgcggccaga ccacctggac ctgatacgta gccgcccata ccgagcacca
    22021 cgtcaggttt gtacgctttc ataatcgccc gcgcctgacg ccaggcgttg aagatacgca
    22081 gcggggcagc tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat
    22141 caatttcgat gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc
    22201 agcgaacttg ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat
    22261 gtccaccggt tccgcctgcc atcaccatta atcgcttccc ttgaccactc atcgtgaacc
    22321 tcgtacaaac gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat
    22381 gatggctgtc gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag
    22441 acctttggtc ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaaactaaa
    22501 ccagatacca atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaatgcttt
    22561 gcggccaatc gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac
    22621 accgacatac cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg
    22681 cagatactcc agtttttgta ccgagttacc taaaccttgt ccccacagtt caccgcgacc
    22741 aaacgccatc aacgattgcg ttaactgata gccgctgcca aagggatctt cccacggatt
    22801 ccagaatgcg gtaacacggc ggatacggta cggttcggcg aggatcagca acacaaccgc
    22861 tgaaatgccc ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa
    22921 catcgccagc gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag
    22981 taacactgcc aacaccagaa ttacgcccat cggtttcagg aagccgcgca ggttattacg
    23041 tacttcgtcg cctttacgca ccagatagtt ggcgatatag caaaacagcg acagttttgt
    23101 cagctccgca ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac
    23161 cgagctaccc actaccagaa cgatcatcag caggatgata gatccgagca gcatcgtggc
    23221 actgtagcgt tgccagaact ccatcggcag acgcagcgta atgatcgcca gaatgaatgc
    23281 caggatcaga tagacgccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg
    23341 ccctatgggc attgacgccg aggtcaccat gataaagcca atcgccgcga ggccgaaggt
    23401 cagccacagt aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc
    23461 catcacccag ccctttagcg ccgtggagat ccagaccaga atactgaatc ctggcaggcg
    23521 cggcattttc aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca
    23581 aactcattgc ctcgttgttc aaagttcttg aattgatcaa ggctggcaca ggccggggag
    23641 agcagaacca tatcgcccgg ctgaacacgc ggagccagca agcgcatcgc ctgttccatg
    23701 gtttcggttt gttctgccac ttccgggcgt agcgccgcca gctgcgcgcc gtcccgaccg
    23761 aaacaataca gacgtacatt atcgccattc aggtaacgcg ccagtgggct aaagtccgcc
    23821 gatttaccat cgccacccag caacaaatgc agcgtgccgt ctacatgcag gccattcagc
    23881 gccgcttccg tactgccgac gttggtcgct tttgaatcgt taacccagcg cacaccgtta
    23941 tgctccagca caacttcaaa gcgatgcggc agaccagtga atgtggttaa cgctttcagg
    24001 ctgctggcac gcggtaaccc tgcggcatct gccagcgcca gcgccgccag cgcattggtg
    24061 tagttatgct gcccggaaag tttcatttct ttcacgttca gcactttctc gcctttcacc
    24121 cgcagccagg tttcaccctg ctgatgattc aggtgatagt cgcccatgtt gacgccaaag
    24181 ctgacgcagc gttcatccgc accgcgaatc ggcattgtta aggcatcatc agcattaacc
    24241 acgcaaattt ttgcgttttc gtaaatgcgc agttttgctg cacgatactg ttgtaaacca
    24301 aacggatagc gatccatatg atcttcagtc acgttcagaa tggtcgctgc taccgcctgt
    24361 aaactggagg tggtttcaag ctggaagctc gacagctcca gcacgtacag ttcgcactca
    24421 tcatccagca gcatcaacgc aggcaggcca atattgccac ccacaccaac gttaaccccc
    24481 gccgctttcg ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggtg
    24541 accgccacaa tcggtgcttg tgcttcacga cagaacagct cgatatcgcc aacgatttcg
    24601 attccggcat cagcggcagc gcttaaggat ggatgcgcca gtgcaatacc gggactggcg
    24661 acaatcagat cggccgccat cagccattca tcattcagac cgcccgtgtg gcgttctaca
    24721 gcttcgggta atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc
    24781 acaccgcgag cgaggaaaaa gtccacgcaa gaaagcccgg tgaggcccag gccgataatg
    24841 acgacatttt taccctggta atcagccatg attaacgtac cttcagcgtt gccagaccaa
    24901 tcaggaccag catcagcgaa ataatccaga aacgtacaat gacgcgcggt tccggccagc
    24961 ctttcagttc atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt
    25021 taaaggagcc gacctgcagg atgacggaaa gtgtttctac cacgaacacg ccccccataa
    25081 tcaccagcag gaattcctga cgcagcagta cggcgataat acctaacgca ccacctaacg
    25141 ccagcgaacc gacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc
    25201 ccagccctgc cccgactatc gcggtacaga caatgaccag ttccccggcg tgtcgtagat
    25261 acggtatgtg taaatagctg gcaaagttca tgttgccggt cgcccacgcc accagcgcaa
    25321 aaccaccggc gacaaatacg gtcggcataa ttgccagacc gtcgagacca tcagtcaggt
    25381 ttaccgcgtt gcctgtaccc acaatgacga agtaagccag cagaatgtag aacagcccca
    25441 gttgcggcat cacatcttta aaaaatggga ccaccagctg cgttgcgggc gtgtctttgc
    25501 caacaaggta cagcgcgaag gcgacaccca gcgcaatgac cgacatccag aaatacttcc
    25561 aacgagcgat caaccctttg gtgtctttac gcactacttt gcgataatca tcaacaaagc
    25621 caataacacc gtaacctacc agcactacca acacgcacca gacgtacgga ttggacgggt
    25681 aagcccacag cagtacggag atcacaatcg ctgtcaggat cataatcccg cccatggtcg
    25741 gcgtaccgcg cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg
    25801 aaagtttttg caaatgagca atcatgcgcg ggcccatcca caatgagatg aacagcgcag
    25861 tcagcaggct gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat
    25921 atttgaccaa atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac
    25981 tacctcttcc atggcagcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc
    26041 agcaatcagt gatttaagac gcgcgataag cgcagtttta tcggaaaaat gttcgccaac
    26101 accgctggca gtgctgatag catggctttg tttgcccacg cttaacacgc ggtcaatacc
    26161 agctgctttt gccgcttcgc ccacctgtat atggcaggct tcgctttcag cgcccagttc
    26221 cgccatatcg cccaccacca gcacgcggta gcccggcatt tcagccagta cctggactgc
    26281 tgcggtcatt gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttccgc
    26341 cagttggatg gggaacagac ggcctggaac agctttcaga tttgccagcc ccgctttgat
    26401 agcatcaagc gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat
    26461 attgtgacgc cccggcaacg gcagcagaac atccacgcta ccggttgggg tttgcagggt
    26521 aaattccgta ccgtgcgaag tcacatggat gttggtggcg gtgaaatcgc tgttggcggc
    26581 attaggtgag aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc
    26641 gttgttgtcg gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc
    26701 tttcgctttc gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt
    26761 gttgaccagc gccgcttccg ggcgagtcag actcacagtc caggcaattt cgccctgatg
    26821 gttcgcgcca agttcaatta ctgcgtaatc gtactctggc gttaagcgca acagcgtcat
    26881 cggtacaccg atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cacactggct
    26941 taaaatcgct gccgtcatct ctttaacgga ggttttgccg gaggaaccgg tcagagcaac
    27001 cacgcgcgcc ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgagt
    27061 atccttgacg attacctgcg gcagatcgat atccagcgga cggttaacca gtagtgcacc
    27121 cgcaccgcca gctttcgcct ggtcagcaaa atcatgggca tcgaaacgtt cgcctttcag
    27181 ggcaacaaac aggcaacccg gcgtcagttt tcgtatgtct gtggttacag catcaagggt
    27241 gatatctgcc ccttttagtt caccgttgag aatatcggta agttggctaa gggttacgct
    27301 aatcatgcaa tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc
    27361 tgattgccaa caatctggta atcctcatga cctttgcccg cgaccagtac cacatcattc
    27421 tctttagcct gcataacggc gcaagtcacc gcttcagcac ggccttccat cactttggca
    27481 tgtccggcat ctaacattcc cgccagaata tcgttgatga tggcacgcgg ttcttcggta
    27541 cgcgggttat cgtccgtcac caccgccacg tcagcgaact cttcagcaat tgcgcccatc
    27601 agtggacgtt tgcctttatc acgatcgcca ccacagccaa agacgcacca cagcttgccc
    27661 gcacagtgca ggcgcgccgc ctgtaaggct ttttccagtg catccggtgt atgcgcgtaa
    27721 tccaccacta ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc acaaaccggc
    27781 tgcaggcgtg cggcagtttt cagcaggtca gccaacggat agcccagcgc cagcagcgtc
    27841 gccagcgcca gcagcaagtt gctgacgtta aaagcgccca tcagatggct ttcaatttcg
    27901 ccatcgcccc agcttgagct aaagcgaatc gtcgcaccgc tgtcgtgata gttcacttct
    27961 gtcgctttca accagcgtcc gtgacagttc ggattgatat gatcttccat tgataccgca
    28021 accgcgtccg gcagttttgc cagccagcgg cggcccactt catcgtcggc gttaataatc
    28081 gcctgaccgc aatgatgctc agaataaagc agccatttcg cggcttcgta gtgttccata
    28141 tcaccatgat aatcaaggtg atcgcggctt aagttggtaa agaccgacgc cgcaaatttc
    28201 aatgccgcca cacggtgctg taccagtccg tgggaggaaa cttccattgc gcaaaaagtc
    28261 gcgccctgat ccaccagccc cgccagctca tgctgaacat cgactgccga accggttgta
    28321 ttttctgtcg ggatcacttt ccccagcagg ccgttaccaa cggtgcccat taccgcgctg
    28381 gtttcgccaa gcagttggct ccactgcgcc agcagctggg tcgtcgtggt tttgccgttg
    28441 gtgcccgtta cgcccacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc
    28501 agtgcagata aacgctcgtt gagctggctg agatagatga ccggtacgcc gtgcatttca
    28561 cggatttcac cgtcggtcgc ctcatctttc gcctctgcaa taatggcagc cacaccttgc
    28621 gctatcgcct gcgggatata tcgacgcccg tccgcctgat gacctactac agctacaaag
    28681 agatcgcccg ccgcagccac acggctgtcg agtgtcatct ctcgcagtgc tcgcgaaggt
    28741 gcgtctggca cccacggagc aagaaggtcg cgcaaattac gatctgccac ctgtcccctc
    28801 gccttgatta atcacaaatt catttttatc gcccgttgtc agcgcatccg gttcgatgtt
    28861 catggtacgc aatacgccgc ccatgatggc accaaagacc ggcgcggaaa cggcgccgcc
    28921 gtagtattta cccgcctgcg gatcgttgat aacaacaacc agcgcgaagc gcggctgact
    28981 cgcaggcgca acgcctgcgg tataagcaat atatttattg atgtagcgac cgtccggccc
    29041 gacctttttc gcggtaccgg ttttaatggc gatacgatag cctttaatcg ccgccttcac
    29101 gccgccgccg cccggtagcg ccacgctttc catcatatgc accacggtgc gaacaatgga
    29161 ttccgggaag actcgttcac cgggaaccgg ggggtcaact ttggtaatcg acagtgggcg
    29221 ataaatgccg tagctgccga tagttgcgta gactcgcgct aactgtaacg gtgttaccat
    29281 tagcccgtag ccgaaagaga aggtggccct ctctatgtca gaccaccgtt gtttttgagg
    29341 atataagcca ctgcgttctc cgaccaaccc caaattggtc gcttttccca gtccaaaacg
    29401 tgagtaagta tctactaacg ctgaggacgg catcgctaac gccagcttgg aaacaccgac
    29461 gttactcgac ttctgtaata ccccggtcag ggttaattcg ctgtagcgtg ccacgtcttt
    29521 gatttcgtgg ccgttaattc gataaggaac ggtattcagt accgagtttt cccgcactac
    29581 gccacgttgc aacgcggtca tcaccaccat cggtttaacc gttgaacccg gttcaaacac
    29641 gtcagtgatg gtacggttac gcatcgcctc tttcggcgtg ccactcagat tgtttgggtt
    29701 gtatgacggg ctgttagcca tcgccagcac ttcaccggtg ttgacatcca ccagcacggc
    29761 gctaccagat tcagctttgt taaaggccac cgcgttgttc agttcgcgat aaaccagcgc
    29821 ctgcaggcgt tcatcaatac tcagcgccag gttgtgcgcc gcctgactgt cggtggaaga
    29881 gatatcttca attacgcgac catagcggtc tttacgcaca atgcgctcac ccggctgccc
    29941 ggtaagccat ttatcgaaac tcttctcgac gccctcaatc ccctgactat cgacgttagt
    30001 aaagccgatg aggtgagcgg tcacttcgcc ggatggataa taacggcgag attcttcacg
    30061 cagatgaatc cccggcagtt tcagtttttt gatgtactct gccatgtcag ggtttacctg
    30121 acgcgccaga taaataaagc gccctttcgg gttggcgtta atgcgggctg aaagctgatc
    30181 cagcggaata ttgagtgcgt tagccagcgc cttccagcga tcaccgacgc tgataccgcc
    30241 agcgtcatgc acttctttcg ggtcagccca aatcgctttt accggcacgc tcaccgctaa
    30301 cgggcgacca gaacggtcag taatcatgcc gcgggaggtg gaaacttgct gaacgcgaag
    30361 agaacgcatg tcgccctctt ttaccagcat atccggggag ataacttgta accacgctac
    30421 gcgtccgagc agaaaagcca gcgccaggag aatacagccg cataacaacg caaaacgcca
    30481 actgataaag ttggcatgtt cttcctgacg ttttggtttc tgcgttttcg ccgctgcttt
    30541 catgcgtcgc gtttatcctt atttttgcac tacgatattt tcttgtgacg gatcaacatg
    30601 ctgcatttgc agcttttccg tggcgatcct ttccacccgg ctatggtcgc cgagcgcatt
    30661 ctcttcaagg atcaggttgc gccattcaat gtctaaagca tctcgctcca gcaccagttg
    30721 ttcgcgctgt gcggtcagta aacgggtatg gtgcgccgtg gttaccacgg tcaccgccgt
    30781 caaaataatg caaatgaaca ggcagagtgg cagcttccca aatcgcaaaa gatcgtcacc
    30841 gataacacca ggcaatgcat ggcgctcgtg gcttcccatc gatcctttaa ctttgcttag
    30901 agcttctgtc actctgctga tcatgcgttc gtcctctctg caatacgcag aactgaacta
    30961 cgggcacgag ggttctcagc cacctcttct tcacccggca ttaacttgcc tagtgctcgc
    31021 agctgacggc cacccagttt tttgagctgc tcttcagtca tcggtaaccc tgccggaact
    31081 tgcggaccgc ggctgttttc acgcataaaa cgtttcacaa tacggtcttc cagcgagtgg
    31141 aagctgatga tcgaaagccg cccacccggg gccagcacgt tgagcgagct ttttagcgcc
    31201 tgctctatct cctccagttc actgtttacc caaatgcgca ccgcctggaa ggtacgggtc
    31261 gcgggatgtt taaacttatc tttcaccggc gttgcagcag ccacgacttc cgccagttct
    31321 ttggtgcggg tcatcggctg ttcgcggtta cgctcgacaa tggcgcgggc aatgcgtttg
    31381 gcaaaacgct cttcaccata ggttttcaat acccaggcga tatcggcttc ttctgcggtt
    31441 tgtagccatt cagcggctga ctgcccacgg gttgggtcca tacgcatgtc cagcggacca
    31501 tcgcgcataa aggaaaagcc gcgttcagca tcatcaagtt gcggtgaaga gacgccaaga
    31561 tcgaggagaa tgccgtcgat cttgccgata agatcgcgct cggcaacgta ttcgcccagc
    31621 gcggagaaag gtccgtggat gatggagaag cgcggatcat caatagtctt cgcaacggcg
    31681 atagcctgcg ggtcgcgatc gatcgccagc aaacgcccct cttcgccaag ctgcgagagg
    31741 atcagacgtg agtgaccacc gcgaccaaaa gtcccatcaa tgtagatgcc atcaggacgg
    31801 atattgaggc cattaacggc ttcatccagc agcaccgtag tatgtttata gttttccatc
    31861 attttataga gacaagtcct gcagtcgctc cgataagtct ccagtagcca actgctctgc
    31921 gtcgatatct tccttgacct gttgatgcca ggttgtttca tcccacagct caaacttgtt
    31981 gaactgtcca accagcatca cttcttttgt cagcccggca tgttgccgca gtactggcgc
    32041 gattaacaat cgacctgcgc catccatctg acattcgctg gcatgaccta acagtaggcg
    32101 ctgcacacgg cgctcaaccg ggttcatgct cgacagacgc gataattttt gctcgataat
    32161 ttcccattca ggcagggggt aaagcagcag gcacgggtga tgaatgtcaa tggtgcaaac
    32221 catttgaccg gcagcgttct caagcagctg ttcccgataa cgggtaggca ctgataagcg
    32281 ccctttgctg tcgagattga ctaacgttgc tccccggaac atgccagcct caccccttat
    32341 tcccactttg ccccacaatt tcccactgaa aggagtttac ggagcggagg aaaagcttgt
    32401 caagccagtc acaaggcata ccgaggtaaa aaacccgatg tttacggcta atatcagcgt
    32461 tgtttatttt acgtccaacg tgcgaagcaa attaacgtta tgaatagctg taagaaaaaa
    32521 tccctcatcg tcatcaccgt tttaaaatga ctcactattt tccacagcaa atataaagtg
    32581 tcagtttgcg acgcgagcgg cattttagga catatcttcc ccggttaaca gtccctgttg
    32641 cgtctgtgtg gcgcttgatg ccagcgggtt tgcgcaaaag caaagaaaag tgtttgttaa
    32701 atcggcaatt catctctgca tgtaacaaaa taatgcaaaa acgcgctatc gatatcactt
    32761 aattattgtg aatttcagct ggtgaggtaa ttaacgggca ggtttaaaag catttatcaa
    32821 taatagccag gaatagtctt atttacttta aggatattga tgtccattcc cgtactcaac
    32881 gcgccagaga gaaattcacc tggcgcgtat ttttgttcgc ggcttagcta cggctgagca
    32941 cgccgcggcg atagagatta cgtttaatgc gcgttaaacc aggttttggc ttacgcggtt
    33001 cgtccaggct tgccaggaca atctccagca cacgctctgc gacatcgcgg tgacgttgag
    33061 ccactgccag caccgggcac tgtaagaagt cgagcagttc gttatcgcca aaggtggcaa
    33121 ttgccaggtc agaaggcagt ttgccgtcac gacgtagcgt gacatccatc accccttgca
    33181 acaacgcaaa cgacgttgtg aacagtgcct gcggcatcgg atgcgtttcc agccattttt
    33241 cgaataactg ggcagccgcc tcccgctcat agctgttggc ataaaggaaa tgcacttcgc
    33301 gaggatcatc tttccaggca gtacggaaac cttgttcacg caggaagctg acagaaagct
    33361 ccggcagcgc gccaagataa agtaccgtct cggcgggaaa cttacgtaac tcttccgcca
    33421 gcatttcggc atcatcctga tcggcaccaa ccacgctggt gaagtgttca cgatcgaggg
    33481 cgcggtccag cgcgacaatc gggaacgggt cgttagccca gcgttgataa aaaggatgct
    33541 caggtggcaa cgacgtcgaa acaataatgg catcaacctg acgctgtaaa agatgctcga
    33601 tacaacgcat ttcgttgtcg ggctgatctt ccgagcaggc aatcagcagt tgataacccc
    33661 tttgccgcgc ctggcgttca agatagttag cgatgcgggt atagctggtg ttctccagat
    33721 cggggatcac aagaccaata gaacgtgtgc gtccagcacg aagcccagct gccacggcgt
    33781 tcgggtggta attgtgctca cgcaccacag ccatgacttt ttcaacggtt ttgtcgctca
    33841 cacggtattg cttcgctttg ccgttaataa catagcttgc agtggtccgc gacactcccg
    33901 ccagccgagc gatttcatcc agtttcacaa ttgccccttg cgtaaaatgt aaaaaccata
    33961 gccattgtac tggtatgggt taaattcatt aacatctaag cgcagaaaat taactgcggc
    34021 aactgctttt attccaggtt cccactgatt tcgcaaaaaa agcccaacgt gacatgttgg
    34081 gctgcaaatt gcgcattgag atcattctca acgcattatt ttatcgccgc gcgaaagtcc
    34141 gactacacca gagcgagcaa cttccacaat tttcgccaca tcgcgaatcg atgctaaaaa
    34201 tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta taaagcgagg gtgtgacatc
    34261 gataatttgc ccacggaata tttccgtatt acgtttcact tcatcacgcc cgtaaccgct
    34321 ggcctgaatt ttcaccagca tgatttcccg ctcaacgtgc gcgccctgcc ccaactcgct
    34381 cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg atttgctcaa gcactttttc
    34441 atcgcccacg gtctggatgg tcatacgcga taatgttgga tcgtcggttg gcgcaacggt
    34501 caggctttca atgttgtagc cacgctggga aaaaaggcca atcacgcggg ataacgcgcc
    34561 tgattcattt tcgagtaaga ctgataatat ccggcgcata atcaggttct ctccgttttg
    34621 cttaaccaca tttcatccat tccgcccccg cgaatctgca tcgggtagac gtgctcactg
    34681 ccatcgacgg tgacatcaac aaacaccagg cgattattgc gcacctgttc cagcgcctcg
    34741 ctaagtttgc tttccagttc ttgcggatga gagatctgga tcccgacgtg accataggct
    34801 tccgccagac gaacgaaatc gggtagtgac tgcatataag attgtgaatg acggccggaa
    34861 tagatcatgt cctgccactg tttcaccatc cccagatagc ggttattgag attcaccacc
    34921 agtaccggca actcgtattg caacgctgta gacagttcct ggatgttcat ctgaatactg
    34981 ccgtcgccag tgacgcaaac cacggtttct tctggcaacg ccattttgac gcccagtgcc
    35041 gcaggtaaac caaaacccat cgtgccgagg ccgccagagt taatccagcg acgcggtttg
    35101 tcgaatggat aataaagcgc agcaaacatc tggtgctgcc cgacatcgga cgtcacgtaa
    35161 gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg cctgcggttt aatcttttca
    35221 ctgtgagtgt catatttcag gcactgacga gcgcgccact gttcaatctg ctgccaccag
    35281 tcgcggatct catccagcgg ttgatggacg gattcttgcg acaggagttc aagcatttgt
    35341 tcgaggacct ggcgagcatc ccccacaatc gggatatcag cagtcacggt tttagaaatg
    35401 gaagtaggat caatatcgat atgcagaacc gtggcatttg ggcagtactt cgccagattg
    35461 ttcgtcgttc ggtcatcaaa acgtaccccg acggcaaaaa tcacatccgc gttatgcatc
    35521 gtcatattgg cttcgtaggt accgtgcatt cccaacatgc ccagtgcctg acgatgcgtt
    35581 gccggaaacg cccccagccc cattaatgag gaaacaacgg gcagattcaa cgcctccacc
    35641 gtttctttta gctgctgatg acagcccgcc gtgattgccc caccgcctac atagacaacc
    35701 ggtttttttg ccgccaccag cgtttgcaga gcgcgcttaa tttgcccttt atgcccggag
    35761 gtcgtgggat tgtaagaacg catactgacc gactccggcc agacataggg taatttgttc
    35821 gccggattta gaatatcttt cggtaaatca acgaccacgg gtccaggacg accacttgcc
    35881 gccagccaga aagccttttt cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa
    35941 ctgtgtttaa ccaccgggcg agaaatcccc accatgtcgc actcctgaaa ggcatcgtag
    36001 cctatcaacg aggtcgctac ctgcccggaa aggacaacta agggaatgga atccatataa
    36061 gcggtggcaa tgccagtaat cgcattggtc gcccctggac ccgacgttac cagcacgacg
    36121 ccgacttccc cggtcgcgcg cgccagacca tcggccatat gcaccgccgc ctgctcatga
    36181 cgaactaaaa catgatcgat accacccacg gtatgcaatg catcgtaaat atcaaggacc
    36241 gcgcctccgg gataaccgaa tacttgttta acgccctgat cgataagcga tcggacgacc
    36301 atctcggctc cagacaacat ctccatggcc tgcctcactg tttgacggaa aaaatgtgta
    36361 aaacacatta accgtccgat cttgcttaag caattggcag cctgagacat aaacacgggg
    36421 taataagaat aaacaggagg aaaggtgaaa agaaacgata aaatcctcca ttacagagga
    36481 ttaaattgat aataaatcgg ctgaatccca caacttacag c
//
LOCUS       DAZUCC010000020        35484 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZUCC010000020 DAZUCC010000000
VERSION     DAZUCC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.020"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..791)
                     /gene="rlmB"
                     /locus_tag="ACHY09_000908"
     CDS             complement(60..791)
                     /gene="rlmB"
                     /locus_tag="ACHY09_000908"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS3883914.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(882..3323)
                     /gene="rnr"
                     /locus_tag="ACHY09_000909"
     CDS             complement(882..3323)
                     /gene="rnr"
                     /locus_tag="ACHY09_000909"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS3883915.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3362..3787)
                     /gene="nsrR"
                     /locus_tag="ACHY09_000910"
     CDS             complement(3362..3787)
                     /gene="nsrR"
                     /locus_tag="ACHY09_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFS3883916.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3992..5290)
                     /gene="purA"
                     /locus_tag="ACHY09_000911"
     CDS             complement(3992..5290)
                     /gene="purA"
                     /locus_tag="ACHY09_000911"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS3883917.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5394..5591)
                     /gene="yjeT"
                     /locus_tag="ACHY09_000912"
     CDS             complement(5394..5591)
                     /gene="yjeT"
                     /locus_tag="ACHY09_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFS3883918.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5673..6677)
                     /gene="hflC"
                     /locus_tag="ACHY09_000913"
     CDS             complement(5673..6677)
                     /gene="hflC"
                     /locus_tag="ACHY09_000913"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFS3883919.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6680..7939)
                     /gene="hflK"
                     /locus_tag="ACHY09_000914"
     CDS             complement(6680..7939)
                     /gene="hflK"
                     /locus_tag="ACHY09_000914"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFS3883920.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8025..9305)
                     /gene="hflX"
                     /locus_tag="ACHY09_000915"
     CDS             complement(8025..9305)
                     /gene="hflX"
                     /locus_tag="ACHY09_000915"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFS3883921.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGEGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9382..9690)
                     /gene="hfq"
                     /locus_tag="ACHY09_000916"
     CDS             complement(9382..9690)
                     /gene="hfq"
                     /locus_tag="ACHY09_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFS3883922.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9776..10726)
                     /gene="miaA"
                     /locus_tag="ACHY09_000917"
     CDS             complement(9776..10726)
                     /gene="miaA"
                     /locus_tag="ACHY09_000917"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS3883923.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10719..12566)
                     /gene="mutL"
                     /locus_tag="ACHY09_000918"
     CDS             complement(10719..12566)
                     /gene="mutL"
                     /locus_tag="ACHY09_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS3883924.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTVEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKMVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPLYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12576..13913)
                     /gene="amiB"
                     /locus_tag="ACHY09_000919"
     CDS             complement(12576..13913)
                     /gene="amiB"
                     /locus_tag="ACHY09_000919"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFS3883925.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPSPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTFLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13932..14393)
                     /gene="tsaE"
                     /locus_tag="ACHY09_000920"
     CDS             complement(13932..14393)
                     /gene="tsaE"
                     /locus_tag="ACHY09_000920"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS3883926.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14365..15912)
                     /gene="nnr"
                     /locus_tag="ACHY09_000921"
     CDS             complement(14365..15912)
                     /gene="nnr"
                     /locus_tag="ACHY09_000921"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFS3883927.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15911..17050
                     /gene="queG"
                     /locus_tag="ACHY09_000922"
     CDS             15911..17050
                     /gene="queG"
                     /locus_tag="ACHY09_000922"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS3883928.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17033..17086)
                     /locus_tag="ACHY09_000923"
     CDS             complement(17033..17086)
                     /locus_tag="ACHY09_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883929.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17320..17395)
                     /locus_tag="ACHY09_000924"
     tRNA            complement(17320..17395)
                     /locus_tag="ACHY09_000924"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17360..17362),aa:Gly,seq:gcc)
     gene            complement(17562..17637)
                     /locus_tag="ACHY09_000925"
     tRNA            complement(17562..17637)
                     /locus_tag="ACHY09_000925"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17602..17604),aa:Gly,seq:gcc)
     gene            complement(17848..18393)
                     /gene="orn"
                     /locus_tag="ACHY09_000926"
     CDS             complement(17848..18393)
                     /gene="orn"
                     /locus_tag="ACHY09_000926"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFS3883930.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18488..19540
                     /gene="rsgA"
                     /locus_tag="ACHY09_000927"
     CDS             18488..19540
                     /gene="rsgA"
                     /locus_tag="ACHY09_000927"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFS3883931.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19637..20605
                     /gene="asd"
                     /locus_tag="ACHY09_000928"
     CDS             19637..20605
                     /gene="asd"
                     /locus_tag="ACHY09_000928"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFS3883932.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDANPLVDDNKDQV"
     gene            20627..23950
                     /gene="mscM"
                     /locus_tag="ACHY09_000929"
     CDS             20627..23950
                     /gene="mscM"
                     /locus_tag="ACHY09_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFS3883933.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLLGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(23981..24295)
                     /gene="yjeO"
                     /locus_tag="ACHY09_000930"
     CDS             complement(23981..24295)
                     /gene="yjeO"
                     /locus_tag="ACHY09_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFS3883934.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24292..24606)
                     /gene="yjeN"
                     /locus_tag="ACHY09_000931"
     CDS             complement(24292..24606)
                     /gene="yjeN"
                     /locus_tag="ACHY09_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFS3883935.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            complement(24669..26171)
                     /gene="yjeM"
                     /locus_tag="ACHY09_000932"
     CDS             complement(24669..26171)
                     /gene="yjeM"
                     /locus_tag="ACHY09_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFS3883936.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(26390..27367)
                     /gene="epmA"
                     /locus_tag="ACHY09_000933"
     CDS             complement(26390..27367)
                     /gene="epmA"
                     /locus_tag="ACHY09_000933"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFS3883937.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27692..29500
                     /gene="frdA"
                     /locus_tag="ACHY09_000934"
     CDS             27692..29500
                     /gene="frdA"
                     /locus_tag="ACHY09_000934"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFS3883938.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29493..30227
                     /gene="frdB"
                     /locus_tag="ACHY09_000935"
     CDS             29493..30227
                     /gene="frdB"
                     /locus_tag="ACHY09_000935"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFS3883939.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30238..30633
                     /gene="frdC"
                     /locus_tag="ACHY09_000936"
     CDS             30238..30633
                     /gene="frdC"
                     /locus_tag="ACHY09_000936"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFS3883940.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30644..31003
                     /gene="frdD"
                     /locus_tag="ACHY09_000937"
     CDS             30644..31003
                     /gene="frdD"
                     /locus_tag="ACHY09_000937"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFS3883941.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            31066..32199
                     /gene="ampC"
                     /locus_tag="ACHY09_000938"
     CDS             31066..32199
                     /gene="ampC"
                     /locus_tag="ACHY09_000938"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFS3883942.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            32288..32821
                     /gene="blc"
                     /locus_tag="ACHY09_000939"
     CDS             32288..32821
                     /gene="blc"
                     /locus_tag="ACHY09_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFS3883943.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32822..33139)
                     /gene="sugE"
                     /locus_tag="ACHY09_000940"
     CDS             complement(32822..33139)
                     /gene="sugE"
                     /locus_tag="ACHY09_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFS3883944.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33314..33460)
                     /gene="ecnB"
                     /locus_tag="ACHY09_000941"
     CDS             complement(33314..33460)
                     /gene="ecnB"
                     /locus_tag="ACHY09_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFS3883945.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33571..33696)
                     /gene="ecnA"
                     /locus_tag="ACHY09_000942"
     CDS             complement(33571..33696)
                     /gene="ecnA"
                     /locus_tag="ACHY09_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFS3883946.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33748..34314)
                     /gene="efp"
                     /locus_tag="ACHY09_000943"
     CDS             complement(33748..34314)
                     /gene="efp"
                     /locus_tag="ACHY09_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS3883947.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34356..35384
                     /gene="epmB"
                     /locus_tag="ACHY09_000944"
     CDS             34356..35384
                     /gene="epmB"
                     /locus_tag="ACHY09_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFS3883948.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8457 a         9738 c         8998 g         8291 t
ORIGIN      
        1 ccagctatag acaaaaaaaa accatccaaa tctggatggc ttttcataat tcagaggaac
       61 tagctgcgct gacgaaccgc ttcaaataag caaattccgg ttgcaaccga aacgttcagg
      121 gaagaaacac ttcctgccat tgggatgctg atcaactcat cgcaatgttc acgggtcagg
      181 cgacgcatac cttcaccttc cgcgcccatc accagcgcca ggcgtccggt cattttgctc
      241 tgatagagtg tatgatccgc ctcgcctgcc gtaccgacga tccagatatt ctcttcctgc
      301 aacatacgca tggtgcgcgc cagattagtc acccgaatca gcggaacgct ttctgccgca
      361 ccacaggcca cttttttcgc cgtggcgttg agctgtgcgg agcgatcttt cggcacaatc
      421 accgcatgaa caccagcggc gtccgcgcta cgcaggcacg cgccgaggtt gtgcggatcg
      481 gttacgccgt cgaggatcag caggaacggt tgatcgagcg aagcgatcag atccggcaga
      541 tcgttttcct gatactgccg tcctggcttc acacgagcga taatgccctg atgcacggca
      601 ccgtcgcttt tctcgtcgag atattggcgg tttgccaact ggataaccac gccctgagac
      661 tcaagggcgt gaatcagcgg taacagacgt ttatcttcac ggccttttaa aataaagact
      721 tcctgaaaac gttcaggggc gcgctccagc agggcctgca ctgcatggat gccgtaaatc
      781 atttcgctca ttaatgtact cgttatttac gttttgccgg gagcgccacg cttgcccggc
      841 ctacggataa ttcaccctct tctgtttgaa gagggtaatg atcactctgc cacttttttc
      901 ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc cgatggcttt
      961 ttcgcttttc tcgcgtcttt cttcgctgct ttcggcttcg gttttttctc gccgcggaag
     1021 gcgctgtctg gctcaaagtt gacctttttg ccgacctgac gccgcttgcc gccttttttg
     1081 cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg cggtgcgcgt
     1141 tcgctggaga tcaggctaaa gtcgattttg cgctcgtcca tattaaccgc ttcgacgcga
     1201 acttccacgc gatcgcccag gcgataagtc tggccgctgg attcgcccat caggcgttgc
     1261 cccacctggt caaagcgata gtagtcattg tccagcgaag agacatggac cagaccatca
     1321 atgaacaagt catccaggcg aacaaagaag ccaaaaccag tgacgctgga aattacgcct
     1381 ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc agccacatcg
     1441 cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag ttgcagcatc
     1501 tcttccatcg aataatggta gccgccggtt tcagtggtat tcccctgatg cccctgctct
     1561 ttcgccaaca gatacttaat ggcgcggtgc agcgtcaggt caggataacg acgaatcggc
     1621 gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt ttccggatcg
     1681 taaatcgctt gtttcatcga gcgcagcagc atggtttgca gcatttctgc atcaggacgg
     1741 tcggcaaccg actccagcag ctctgcgtag tcgcgcggtt ccggcttgtt accgcccggc
     1801 agttccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc ggtgctcggc
     1861 ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg cgccgccgag
     1921 atattcgcca gaatcatgca ctcttcaatt aacttgtgtg cgtcgttacg ctgagtctgt
     1981 tcgatacgct caatacggcg ttcggcgttg aaaatgaact tcgcttcttc gctctcaaat
     2041 gagatcccgc cacgttcttc acgggcttta tccagcactt tatagaggtt atgcaactct
     2101 tcgagatgct taaccagcgg ggcatactgc tcgcgcagat cctgatcgcc ttgcagtata
     2161 tgccagactt tggtgtaggt cagacgcgcg tgagaactca tcaccgcttc gtagaatttg
     2221 tagccagtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca caggcggtct
     2281 acctgcgggt tgagcgagca caggccgtta gagagcactt ccggcagcat cgggataacc
     2341 tgcgaaggga agtacaccga cgtaccacgg ttacgcgctt ctctgtccag cggtgttggc
     2401 ggacgcacat agtaactgac gtcggcaatt gcgacccata aacgccagcc gccgccgcgt
     2461 tttttctcac agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc aatggtgacc
     2521 agcggtaaat cgcgcagatc gacacggccc gcttttgctt cttccggcac ttcttctttc
     2581 agcccggcaa cctgttgctc aacagcctgc ggccagatgt acggaatttc atgggtacgc
     2641 agagcaatat caaccgccat gccggtgccc atattatcgc ccagcacttc gacgatttta
     2701 cccaccgctt tggtacggcg agtcgggcgt tgagtcagtt caaccaccac cacaaagccc
     2761 atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag acggctatcg
     2821 tcaggaacca caaagccgac gcccgcttcg gtgaagtagc gaccaacaat ctggctggtt
     2881 tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc agcgcccagc
     2941 ggctgagcca gcacctgatc gccatgaatg caggttttca tctgctcgct ggagagatac
     3001 aaatcatctt tacgcccttc aactcgcaga aagccgtagc catcacggtg gccaataacg
     3061 gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacggcg agtgaagacc
     3121 agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag ctgctcttcg
     3181 ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc acgtttggtt
     3241 aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc agcttcgcgt
     3301 tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat gaagattttc
     3361 gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa tcggcaagcg
     3421 tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc tgtttcaacc
     3481 gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat aagggctcca
     3541 gctcgcgcac cacatcacca atacgtattg cactcgccgg tttacccaag cgaatgccgc
     3601 cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg attattttga
     3661 ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg gtcatccgcc
     3721 cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg aaactcgtta
     3781 actgcacatc aacctcaaaa gggaaatcgg ggaaaaatac atttatattg atgataaacc
     3841 agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc gaggatttaa
     3901 cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac tgttagcccg
     3961 gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg cgcagaatca
     4021 tggtttcagt acgatccgga ccagtagaga tgatatcgat cggcacaccg gtcagctctt
     4081 caatgcgctt gatgtagttc agtgccgcct gcggcaggcc gctacgatct ttcacgccga
     4141 aggtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct ttccagtcgt
     4201 cagctgccag cggagtggta gtcacttcac gaccatccgg catacggtaa gccacgcaga
     4261 gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag ccagacagag
     4321 agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta cgacgacgac
     4381 gacccgtagt tgcgccgaat tcgttaccct gcttgcagag gaactcgcca gtttcatcaa
     4441 acagttcggt cgggaacgga cctgcaccca cacgagtgga gtaagctttg aggataccca
     4501 gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca ccagcagtgg
     4561 tgttggaaga agttacgtac ggatatgtac cgtggtcgat atccagcagc gtaccctgcg
     4621 caccttcaaa catgacgaaa tcgccacgct gacgcgcctg gtcaagcagg tcagaaacgt
     4681 caacaaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga actttctggt
     4741 aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat tccatcactt
     4801 ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc agaccgcgac
     4861 gtgccacttt atcttcataa gcaggcccga tacctcgacc ggtggtgccg atcgctttcg
     4921 cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg atcagcggac
     4981 atgcttcgga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc agttctttca
     5041 tctctttcat cagcgcagcc ggagacagca caacaccgtt accgatgatg ctggttacat
     5101 tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg ttgattacga
     5161 gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc cgttcagtca
     5221 gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt acgacgacgt
     5281 tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat cgctttttca
     5341 gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt tgttcagcca
     5401 atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc accgccaaaa
     5461 cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt ccatgccttc
     5521 gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc cagccagatt
     5581 gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct gaggatgcgg
     5641 tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt cggcgtcttc
     5701 atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt gccagagaag
     5761 ctgttctcat aagcacgcag gctacggata aatgcgtaga agtccggatc tttactgaat
     5821 gcatcagcaa acagtttggc tgcttctgca tcgccttcac cacgcatgat gcggccctga
     5881 cgctcagctt cagccagcgt tctggtcact tcatagtcgg cagtcgcgcg cagtttttcc
     5941 gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc ggcgcgcata
     6001 cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttaat acgcacatcg
     6061 acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac tttgcccttc
     6121 gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc cggggtagta
     6181 acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc gagggtcaga
     6241 cgaccacggg aatcggtgac gatgtctttc acgtccaggc gaccaatttc agaacgcaga
     6301 cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc accacccgtt
     6361 gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc gacgatcagg
     6421 tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg aatacgtgcg
     6481 tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagacccgg ctcataaacc
     6541 agaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat accgcgctca
     6601 ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat gataatcgcg
     6661 ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc tgcgcgttgg
     6721 cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt gctccactgg
     6781 ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta tcgctcttcg
     6841 ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc aagttgccac
     6901 ctttatcgtt aaccagcact ttgcgggtgt tgcccaacac tttttccatc gtctcgatat
     6961 acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt ttagcaaagc
     7021 gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc gcctcttcga
     7081 ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac gcttctgctt
     7141 cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac gccgctttta
     7201 cttcttccgg cggacgagct gcctggaagt tgacgtccag cagcgtgata cccatgtcat
     7261 acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg gtacgacctt
     7321 ccgtcagaat gcggtccatg gtgtatttac cgataacccc acgcagggcg ctgtcggtag
     7381 cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc ggatcggtga
     7441 cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc agcatcacac
     7501 cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg tcgataaacg
     7561 tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag cgtgttacca
     7621 cgccacgttc ggcttcttta atggtataga aaccactggc cgcccagata atgacaatcg
     7681 ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct tgcgatgaac
     7741 tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt ttgctcagtt
     7801 tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg tttccatttc
     7861 cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt ccgttattac
     7921 cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc cctgttgtct
     7981 ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat caggtaatcg
     8041 atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat acgaacttgc
     8101 agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag ctgataaaaa
     8161 cgacttctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc cacctcgccg
     8221 gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc cctctccggt ctgtgcggaa
     8281 agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc gaaatcttcc
     8341 agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc gatctcttca
     8401 agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc atcaatgacg
     8461 tgcagcagca atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc cgccaccaga
     8521 tcgtgcggca ggtggcgaat aaaccctacg gtatctgcca acacggtttc accgacatct
     8581 gcgacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc tgccgcgtag
     8641 acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt atatcccacc
     8701 agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc acgctgcttt
     8761 tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa acgacggtcg
     8821 gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg tctttcaagg
     8881 tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag ctcaacctgc
     8941 aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag gccggtgcgg
     9001 tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg gctcagggca
     9061 tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat ttcaactgct
     9121 ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt aatcacctgc
     9181 aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc ttccatatct
     9241 ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc ataacggtca
     9301 aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg gctccccgtg
     9361 taaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg cggaagtatt
     9421 ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt tgttactgtg
     9481 atgagaaacc gggcgagacg ggacaacagt agaaatcgcg tgcttgtaaa ccatctggct
     9541 gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc cttgcagctt
     9601 aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg cgttcaggaa
     9661 cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc ttatttgtac
     9721 tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta cacattcagc
     9781 ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ttgttctggt ttttcactgt
     9841 caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc gccaactgtc
     9901 tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct tcaaggtaag
     9961 accacatctg gcgataaccc acgcaacgaa tggaaggcaa atccgtatgc aaatctcctc
    10021 gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga tgaaaacgct
    10081 gctcaattcg ttgatggagc agttcacggc tcgccggggc gatggcgaac tgatgcacct
    10141 gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta cccgaaatga
    10201 aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc cttgctgccg
    10261 caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc tctgccgcct
    10321 gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac aacccttcca
    10381 gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc cccgccgcgg
    10441 tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag taagcctgcg
    10501 acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca gcgttcggct
    10561 tcgccgtccc gatatccatc cctttgtaaa taagggcaga atcaacgctt atcaactcta
    10621 ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc gtcggcccca
    10681 tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt cagggctttt
    10741 atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt cacaagttgc
    10801 ggacataacc gttccacgtc cgccagcagg gttatggcct gcgccattga ccactgcgca
    10861 tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc gaatacggac
    10921 tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg ttggcgtaag
    10981 ggtaaaggca cggccctgat ggtcacatgc tgcgcatcgg actggaaatc aatacccaat
    11041 tccgccaggg cagactgcgc tttttctaat gcagattttt cttcgccaga aacttttagc
    11101 cgcagcggaa tcagcaacgg ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc
    11161 tgacgcagcc aacgttctgc cactggcaag gctaaaagtg aaatgttgcc gtcgcgctcc
    11221 agcaacgcac agtcggaatg gacgatagtc agtacccgac caaagctttg actgttcgcc
    11281 gcaagtgcag gttcctgcgg ttctggcgct tttggttttt gcatcggcgc gggcgtttgc
    11341 aaaagctggc gatacacttc accttgctgt ttctggtaac ctggctgcgc attcggccag
    11401 ggggcagccg gacgactgcc tgaggcgggc gcaggagtgt aaagcggagc tactggctca
    11461 cgaactgccg gttcagcaaa atgattgcgc cccgccgcca cgcggttttc cgggatggcg
    11521 cgcggtgcag gttgtggttc atcgtccagc ggtagcggcg tttccagttg ctgttgtagc
    11581 acgctcagca cgccctgata gataaagtca tgcaccagac gcgactgatg gaaacgcact
    11641 tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc cagatacagc
    11701 acaaacgccg gttgttgatc ggcccccagt ttgtcttcgc aggcctggcg gatcgcatga
    11761 ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat ttccgccagt
    11821 gcgggcgtgg tgtgattcgg atctgccacc cagccgcgca gcgtaagatc gccgtgttgc
    11881 cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcccc taaacggcgc
    11941 tctttttgtc caccttccgg cacggcgcgg tactggcgga ccattttccc gttatgcgac
    12001 aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatctc atcaatgtgg
    12061 ttaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga
    12121 tccagcacct ccagcgtagt ccccacagga tgcgccgccg gttttaccgt cacgtccata
    12181 tcgcgccctt cggcataggc ctgccaggct tcctgctgtt ctacggtgcg tgaagtgagc
    12241 gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc caggctgata
    12301 atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc cagcgccagc
    12361 gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag tttcgccccg
    12421 ccacgttcga tatcaatatc aatgcgcgtc gcacctgcat cgaggctgtt ttccaccagt
    12481 tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg gttcgccagt
    12541 tgtggcggta agacctgtat tggcatcaat cgtccttagt ttggcagcgt acgatctggc
    12601 gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg catcggatgc
    12661 gcaaggaaat aattgcgcag acctttataa atggcttctg ccagctgttg ttggtaatcg
    12721 tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc gaccagtact
    12781 gatgggatat ccggcgaacg cagaacgcca aggctggcgt gttctggtcg acgtttatgt
    12841 atttcgccaa tgcgttgcaa ctggctgatc atactggtcg ctacatcata ccctacacgc
    12901 tgggaatgac cgaactgtaa atccagcacc gcctggctta aatagggatc agactgactg
    12961 ttcgccagca catcgcccgc tccgcccagc agctccgact gtttctcgtg ctgctccagc
    13021 cagctggcca tttcgctgtt ggcgcgacgg ttagagagca cccatacgga agcgccagtc
    13081 gcactgcggt ttggtgcggc atcagcgtga atcgacacga ggaaattggc gttttgctta
    13141 cgtgccacat cgctgcgccc catcaccgag ataaagtaat ccccgtcacg ggttaaaacg
    13201 cctttaaaca ttggatcgtc attgagaaaa gtacgcaatt tacgtgcgat ggcgatggtg
    13261 acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc gccgtgtcct
    13321 gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc tgccggacgc
    13381 gttaccgtat tactgctgat aacacctgta gtgcggttac tttccgtttt aaacggattg
    13441 cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg tttagcaacc
    13501 acaggcggcg aaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac gacagtgtaa
    13561 ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggttag atcgaccacc
    13621 agccgtagcg tttgtgcatc tttaggcgtt ccggagcgaa tcgccttcac cagattattg
    13681 ccgctaaaca acaacggcag tccctgaatc acgcccgttt gtttgatatc gagcgccacg
    13741 gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact caacgttatc
    13801 cgcgcctgct gattgccgtt agaaacctga atatcagaga gcgtcgcggc acccaccggc
    13861 gcgcacagca gcagcaacgt cgctaccaac caatttctga tgcgatacat catcccgcca
    13921 cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga ggaaaccgca
    13981 ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac atccgggtca
    14041 ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc gttggcaaaa
    14101 taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta caaatcaaag
    14161 tgataaacca ttaaattgtc gagcgtatag ggttcgacca gcgtataagt ggggcttttg
    14221 acattaccct gatggcccaa agcctgcaaa aagccccggc taaaggtggt tttacctgcg
    14281 cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc tacccgctcg
    14341 cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat catggttttt
    14401 atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg ttgccagcat
    14461 cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg cgacacagcc
    14521 cgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa taataccaga
    14581 gagcacatcg cccatgccgc cgctcgccat gcctgcattc ccggcatcaa taatgcctaa
    14641 agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta cgccgccata
    14701 acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag cgacggaaca
    14761 gcctaacaac cgtgcggcct cgccaggatg tggcgtgatc acgcgattgt gacgcttatc
    14821 gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt tgcgaaaatt
    14881 ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg gaccaatcac
    14941 caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt catgcaccat
    15001 caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca gtactcggac
    15061 taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc ccgccgtgcc
    15121 gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag tcgggcgacg
    15181 cggtatcagc cactgagaaa gttgttctgc cgaaaaccgc tgaattttcg tctcctgacc
    15241 tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtccggtaa catcccgcgc
    15301 ttttccagtg agcaagcctg gtttcagcgc aataaaagtg atggtgtgat cggcgttgat
    15361 taccgcgcct ggcgtagcgc cggtttcagc cagaaggccg gaagggatat caaccgccac
    15421 aatcggcgca gggtgagtat tagcgtggtc gattaactgg ctaatggatt cgcggggcgc
    15481 ttgctgcaag ccggtgccga gcagcgcatc aacaatcaga tctaccgatt cgggccagac
    15541 aatattcgaa gcatggatct cgcctcccgc gtttaaccat gcttcgcgtg ccagcgcggc
    15601 ctcttccggc aacggtttgt cactttcctg ggccagcagc gtgacatcaa taccgatcgc
    15661 tttggccagc cgcgcgacca cgtagccatc gccgccgtta ttaccatgac cgcacagcac
    15721 cagccagtgg cgggcgtcag gataagccga acgacacacc tggaatgcgg cttcgccagc
    15781 gcgaagcatc agctcataga gtgtgagccc cagcgcatct gccgcctcgc gttctccgcg
    15841 gcggatatcg tcggcgtacc agacggtgtg tggtatactt acggggtttt tcttcattgt
    15901 atggtccgtc atgtcagagc ccctcgatct caatcaatta gcgcaaaata ttaaacagtg
    15961 ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca gcgcgtccga
    16021 gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact ggatggcgcg
    16081 tcacggtatg ctgcgtgctc gtcctcacga attattgccc ggtacgctgc gcgtgatcag
    16141 cgttcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga aaaatcccaa
    16201 actcggctat gttagccgtt atgcgctggg ccgtgactat cacaagcttc tgcgcaaccg
    16261 actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt ttagaccgtt
    16321 tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagcaggtc ttggctggac
    16381 aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt taggcgaatt
    16441 gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg gtaaatgcgt
    16501 ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg tcgatgctcg
    16561 ccgctgtatc tcttatctca ccatcgaact ggaaggggcg atcccggaag agttgcgacc
    16621 gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc cgtggaatcg
    16681 ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac acgcaccgga
    16741 actcattgag ttattcgcct ggagtgaaga gaagttttta aaagtcacgg aaggttcggc
    16801 gattcgccgt ataggtcacc tgcgttggct gcgtaatatc gccgtagcat taggcaatgc
    16861 cccctgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc acccacttct
    16921 tgatgagcac atagcgtggg cgattgcgca gcaaatcgag aggcgaaatg cgtgcgtggt
    16981 cgaagtgcaa ttacccaaaa aacagcgtct ggttcgggtg attgaaaaag ggttaccgcg
    17041 tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgtattgca attcaagacg
    17101 aataaaatcg tcaagtgatc gactcgacaa aacgaaatct taatttttaa cttatctatc
    17161 aataggttaa aaaaatacta ttcaccgtgc gaagtttctt tgaaattaag attacggcct
    17221 gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac cagcaataac
    17281 gcatcgcgtc gctgtggata ttttattgag agaagaattt ggagcgggaa acgagactcg
    17341 aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt cccgcttaat
    17401 cttcgtcttt cgaaccgcta ctgccttggc tgccttggct gccttcactc accttagtca
    17461 cttacttttg taagttccca aggattcgtt cagttgccgc cttgtcgcaa ttcgaaatac
    17521 tgcgattggg tggtctgtgc tttgcagcac ttttcaaatt ttggagcggg aaacgagact
    17581 cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta ttcccgcgta
    17641 ctacttaatt ttgctttcgt aattttcgca tttctgcgtc gttacgggag gcgcattata
    17701 cgagaaatct ttccctctgc aaccccccta aaaaattttt tttgagtttt tatctcaagt
    17761 gctgaaatta tcagcaagac gttcaattta gcgtcaaaat cacgccgccg caagtggatt
    17821 ccggcgctta cgtgaccagg aaaaatttta cagcttgata aaatgctcgc ggtagtaagc
    17881 cagttctgcc accgactcac ggatatcatc catcgcctga tgcgtcccct gcttggtaaa
    17941 accatcaaga atttccggct tccagcggcg cgccagctct ttcagggtgc tgacatcgag
    18001 ataacggtag tggaagtagg cttccagctc cggcatatat ttaaacagga agcgacgatc
    18061 ctggccgatg ctgttaccgc aaatcggcga ttttcccgca ggcacccact gttttaagaa
    18121 ttcgagcgtt gccagttcag cttcacgatc gcccatcgtg ctcgctttca cgcgctccac
    18181 cagcccgctg gcggtatggg tgcgcacgtt ccagtcatcc atcagcgcca gctgttcatc
    18241 agactggtgt actgcaatgg tcggcccttc tgccagaata ttcaggttgg catcggtcac
    18301 cagcgtggca atctcaataa tgcgatcgcg ctcgggatcc agaccggtca tctcaagatc
    18361 gatccaaatc aggttgtttt cattggcact catgctattt tccacccatt cacagtgact
    18421 atattgatcc acaattgcgt gtatcataga tgttttgccc atcaggggcg accaggagtc
    18481 tgtacgattg agtaaaaata aactctccaa aggccagcag cgccgcgtga acgccaatca
    18541 ccagcgtcgt cttaaaacgt ctaaggagaa gcccgactac gacgacaatc tgtttggcga
    18601 gcctgatgaa ggtatcgtca tcagccgctt tggtatgcac gctgatgtgg aatccgccga
    18661 tggcgacgtt caccgctgca atattcgccg taccatccgt tcgttggtga ccggcgaccg
    18721 cgtagtctgg cgtccgggta aaccagcggc ggaaggcgta aatgtcaaag ggatcgtgga
    18781 agcggtgcat gagcgcacct cagtgttgac gcgcccggat ttttacgacg gtgtgaaacc
    18841 tattgctgcc aacatcgacc agattgtcat tgtctccgcc attttgccgg agctgtcgct
    18901 caatattatc gaccgttacc tggtggcctg cgaaaccttg cagattgagc cgattattgt
    18961 gctcaacaag atagacctgc tggacgacga aggcatggca ttcgtcaacg agcagatgga
    19021 tatctaccgc aatatcggtt atcgcgtatt gatggtttcc agtcatactc aggatgggct
    19081 gaaaccgctg gaagaggcgt tgaccgggcg catcagcatt tttgccgggc agtctggcgt
    19141 tggcaaatcc agcctgctga acgcattgct gggtctgcaa aaagagatcc tgaccaacga
    19201 tgtctctgac aactcgggtc tcggtcagca caccacgacc gccgctcggc tgtatcactt
    19261 cccgcacggc ggtgatgtga ttgactcccc aggagtgcgt gagttcggcc tctggcacct
    19321 ggagccggaa caaatcactc agggctttgt cgaattccat gactacttag gtctgtgtaa
    19381 atatcgcgat tgcaaacacg ataccgatcc gggctgcgct atccgggaag cggttgagga
    19441 aggaaaaatc gcggaaaccc gtttcgaaaa ctatcaccgt attctggaaa gcatggcgca
    19501 ggtaaaaacg cgtaaaaact tttctgatac ggatgactga caattaagct aaccgtcgtt
    19561 agaatcgtcc cctttttttc aggatccggc atgtaggccg gatcaggaac gacaaaacaa
    19621 tggcctggag gctaccttgt taaattcatt taaactttcg ctacagtaca ttctgccgaa
    19681 actatggctt actcgcctgg cgggttgggg cgcaagcaag cgggcaggat ggctgacaaa
    19741 actggttatc gatctgttcg ttaaatacta caaggtcgat atgaaagagg cgcaaaagcc
    19801 ggacaccgcc agctaccgca cctttaacga attctttgtc cgtccgctgc gtgacgaagt
    19861 acgcccaatc gataccgatc cgaatgtact ggttatgcct gccgatggcg ttatcagcca
    19921 gttgggtaaa atcgaagaag ataaaatcct gcaagccaaa ggccacaact acagcctcga
    19981 agccctgctg gctggcaact atctgatggc ggacctgttc cgcaacggta cgtttgtgac
    20041 cacttacctc tccccacgtg actaccaccg cgtacacatg ccgtgcaacg gtattctgcg
    20101 tgagatgatc tacgtgccgg gcgatctctt ttccgttaac catctcaccg ctcagaacgt
    20161 gccgaatctg tttgcccgta acgaacgcgt gatctgcctg ttcgataccg aatttggccc
    20221 aatggcgcag attctggtcg gggcgacgat tgtaggcagc attgagacgg tctgggcggg
    20281 caccattacg ccgccgcgcg aaggtatcat caagcgctgg acctggcctg ccggggaaaa
    20341 cgacggttct gtggcactgc tgaaaggcca ggaaatgggt cgctttaaac tcggttccac
    20401 cgttatcaac ctgtttgcac cgggtaaagt gaatctggtt gagcaactgg aaagcctgtc
    20461 tgtcacgaaa attggccagc cgctggcagt atctaccgaa acctttgtta caccagacgc
    20521 tgaaccggcc ccgcttcctg ctgaagagat cgaagcagaa cacgacgcca acccattggt
    20581 tgacgacaac aaagaccagg tctaatccat caaaggaaac gctgacgtgc gcctgattat
    20641 cacttttctg atggcctggt gcctcagttg gggggcgtac gccgcgacgg cccccgatag
    20701 caaacaaatc tcacaagaac tggagcaggc aaaagcggcg aaacccgcgc agccggaagt
    20761 cgtagaggcg ctccagtccg ccttaaatgc gcttgaggaa cgaaaaggtt cccttgagcg
    20821 catcaagcaa tatcaagaag tcattgataa ttacccgaaa ctttccgcta ctctgcgcgc
    20881 acaattaaac aacatgcgtg acgaaccgcg cagcgtgtca ccgggaatgt ctaccgacgc
    20941 gctgaatcag gaaattctcc aggtcagcag ccagttgctg gataaaagcc gtcaggccca
    21001 gcaagagcag gaacgcgccc gcgagattgc cgattcgctg aatcaactgc cgcaacagca
    21061 aaccgatgcc cgccgtcagt taaatgaaat cgagcgccgc ctggggacgc ttaccggcaa
    21121 ttccccgctc aatcaggcac aaaatttcgc gttgcagtct gactccgcac gcctgaaagc
    21181 gctcgtcgat gaactggaac tggcgcagct gtctgccaat aaccgtcagg aattagcgcg
    21241 cttacgctca gagctggcgg aaaaagagag ccagcaactg gatgcgtatt tgcaggcctt
    21301 acgtaatcag ttgaacagcc aacgtcagct tgaggcggag cgggcgctgg aaagtaccga
    21361 attactggca gaaaacagtg ccgatttgcc gaaagatatc gtcgcgcaat tcaaaattaa
    21421 ccgcgaacta tcggcagcgc tgaatcaaca ggcgcagcgg atggatctcg ttgcctcgca
    21481 acagcgtcag gctgccagcc agacgttaca ggtccggcag gcgttgaata cgctgcgtga
    21541 acagtcgcaa tggctgggct cgtccaacct gctcggcgaa gcgttgcggg cgcaggtggc
    21601 acggctaccg gaaatgccaa aaccacaaca gcttgatacc gaaatggcgc agttgcgtgt
    21661 gcaacggtta cgttatgagg atctgctaaa taaacagccg ctgctacggc aaatccatca
    21721 ggccgacggt cagccgctga ccgccgagca aaaccgtatt ctggaagcac agctacgcac
    21781 tcagcgtgag ttgctgaact cattgttgca gggtggagac acgctactac tggaactgac
    21841 caagctgaaa gtctccaacg ggcaactgga ggatgcgctg aaagaggtga atgaagcgac
    21901 gcaccgctat ctgttctgga cctctgacgt gcgcccgatg accatcgcct ggccgctgga
    21961 aatcgcccag gatctgcgtc gtctcatttc gctggacacc ttcagtcagt tgggcaaagc
    22021 cagtgtaatg atgctgacca gtaaagagac gattttgccg ctgcttggcg cgttgattct
    22081 ggtcggttgc agtatttact cgcgccgcta tttcacccgt tttcttgaac gttcggcggc
    22141 gaaagtcggc aaagtgactc aggatcactt ctggctgacg ttacgcactc ttttctggtc
    22201 gattctcgtc gcgtcaccgc tgcctgtgct gtggatgacg ctgggttacg gcttgcgcga
    22261 ggcgtggcct tatccgctgg cggtcgcgat tggtgatggc gtaacagcca ccgtgccgct
    22321 gctgtgggta gtgatgattt gcgccacctt tgcccgcccg aacggcttgt ttatcgctca
    22381 ttttggctgg ccgcgcgaac gggtttcccg tgggatgcgc tactacctga tgagcatcgg
    22441 gcttattgtg ccgctgatta tggcgctgat gatgttcgat aacctcgacg accgtgaatt
    22501 ctccggttcg ctgggacggc tttgctttat cctcatttgc ggtgcgctgg cggtggtcac
    22561 tctcagtctg aaaaaggccg ggatcccgct gtatctcaac aaagaaggca gcggcgacaa
    22621 cattaccaac catatgctgt ggaacatgat gattggcgca ccgctggttg ccattctggc
    22681 gtcggcggtg ggttatctgg caacggcgca ggcgctgtta gcgaggcttg aaacctcggt
    22741 tgccatctgg ttcctgctac tggtggttta tcacgttatc cgccgctgga tgctgatcca
    22801 gcgccgcagg ctggcgtttg accgggcgaa gcatcgccgg gcagagatgt tagcgcagcg
    22861 tgcgcgtggc gaagaggaag cgcatcatca cagtagcccg gaaggagcaa ttgaagtcga
    22921 tgaaagcgaa gtcgatctcg atgccatcag tgcgcaatcc ttgcggctgg tgcgctcaat
    22981 tttgatgttg atcgccctgc tttcggtcat tgtgctgtgg tcagaaatcc attccgcttt
    23041 cggcttcctc gaaaatattt cgctgtggga tgtcacttcc acggtacagg gcgtagaaag
    23101 cctggagccg attaccctcg gtgcggtgct gattgccatt ctggtgttta tcatcaccac
    23161 gcagctggtg cgcaacctgc ccgcgctgct ggaactggcg attctgcagc acctggattt
    23221 aacgccgggt acgggctacg ccatcaccac catcaccaaa tatctgctga tgctgattgg
    23281 cgggctggtc ggcttctcga tgattggtat tgagtggtcg aaattgcagt ggctggtcgc
    23341 cgcgctcggt gttggtctcg gttttggttt gcaggaaatt ttcgccaact ttatctctgg
    23401 cctgattatc ctgttcgaaa aaccgattcg cattggcgat acggtaacga tccgcgatct
    23461 taccggtagt gtgacgaaaa ttaacacccg cgccaccacc atcagcgact gggaccgcaa
    23521 agaaattatc gtgccgaaca aggcgtttat taccgagcag tttatcaact ggtcgctctc
    23581 tgactcggtc acgcgcgtgg tgttgacgat tccggcccct gccgatgcca acagtgaaga
    23641 agtgacggaa atcctgctca ccgcagcgcg tcgctgctcg ctggtaatcg ataacccagc
    23701 accggaagtc ttcctggtgg atctgcaaca ggggattcaa attttcgagc tgcgtattta
    23761 cgccgctgag atgggtcacc gtatgccact acgccatgag attcaccagc taattctggc
    23821 aggattccat gcccacggta ttgatatgcc attcccgccc ttccagatgc gtttggaaag
    23881 cctgaatggt aagcaaacgg ggagaacatt aacttctgcg ggcaaaggtc gtcaggcggg
    23941 aagtttgtaa ggtctttgtg gccggatgaa cccatccggc ttataagcaa aactgatagc
    24001 gcagaaaaaa ctgccacagc caatagccga aaatagccag cgcggtggcg tacataaacc
    24061 agccgcgttt ttttcgccac aaagcgaacc agagaaaagg gaaaaagagc ggtaacgtca
    24121 ttatcacacc gacatcacgg gtatcgtcat cttcgagata catcagcaca tcgcagacgt
    24181 tattaatccc gcgtccatca atcatccact ctttgtccag cgcggaagtg atggttatcc
    24241 agaggaacgc cacaataaac cagatgcagc acaagataaa catgcgcgca ctcatgagat
    24301 aatcggtcgc ttaatttcgc tatagcgcat acgaaacaaa tcgccgtatc ccactaattc
    24361 tccgcgaaaa accagacatc taataagata agcgcaatag atatccggca ggtttcccca
    24421 ccgcggacca tcgcgctctt tgagggtatt aatagtgtta cccaccacca tcgctacttt
    24481 gcgggtgtgg cgggtatcga caaaagagag gaccgtttgc tctatttccg ccacatcccc
    24541 ggcagaaaat tgcagctccc tgatttcatc ttcggttatc actggatccc gactagcgtc
    24601 gtccataacg tcttcctttc cagacaactc atcgccggag agacgatgag ttgcaggatt
    24661 tcatatgatc agtcgagagc taactcaggt ttgttcgcca tgcgactgca atagttctgg
    24721 taaatcgcca tcgctaacag cgagaagaag acagggccgc caatcatcca caatgtgctg
    24781 tcccagtctc cagcttcaac cacaggttga atgatggtga agacgttcgc aaatgtcacc
    24841 accagtacga caaccactgt tgcaatcatt gccgacatat gcgttttgaa aatcacaaac
    24901 ggtctgtcga gatcctgacg tgctttaaag aatgggaaag ccagcgcgag gaacaggtaa
    24961 ggaagcgtca tagacacgtt cgccatcagc gtcagcttgt taaagaacgc cgatgcggta
    25021 ccgccaccaa acgaaaccag caggatgaag acagtaacca acccgcactg catccacatg
    25081 gcgatagacg gcatccccat cgcattcagg cgcgtcatcg gttccggcca caacgctttc
    25141 ggcgtcccct ggatgatggc tttcagcggt gaatagcaaa gcgtaaagaa cgcaccggta
    25201 taagcgagga acatcgaaag tccggtaata cgcgcaaacc atacgcccag cgacaatgac
    25261 gcttcaggtg acaaatgcag tgcgttaccc agcgtcaccc cgaggctctt catcagcaca
    25321 taggtaatat tgccgaggtt aacggaacca ttacttaata cctgctgcca gtttgtgctg
    25381 acgccccata aaaatattgc cagcgaataa ccgattgaaa taacaatagc ggcaaaaaca
    25441 atacctttgg caaagttctt ttctggattc tccgttttat cgaccagacc accaaccgct
    25501 tcaattccac cataggcaaa aatagcaaat accacaaacg atagcattgc cagaccggac
    25561 tgataacctg ggttcggtga tgcaaggaaa ttaatatcct gcgcgaaatg cccaccattt
    25621 aataacaaaa tagtaatgct tactaacagc aatactaaat tcagacacat tactgcaata
    25681 ccgcccaccg cagtaatgcg ggcaatttta ttaatccctt ttgaagcaac gacggtgacc
    25741 agaatcatcc atgccactgc cagcagacca accacctgcg taggctccag tccggcaata
    25801 cgccagtgct gggtcatgtc gctaccatag aggaatgttg agaacggtac ccaaactttc
    25861 gcggaggtac tcaccatcca gatgatataa gaggaaaacc acataaacgt accaatgaag
    25921 gcaaaacgtg gtccgacact attattcatc caggaataga taccgccttc ttctttgcga
    25981 taagcagctc ccatttcagc catcattaag gcgaatggaa taaagaataa caatgcagaa
    26041 aatatataaa agggaatcgc actataaccc attaagtaat aagccgatgg gctattggca
    26101 aatccaaata cggaagtaaa gatcatcaat atgagtccta tcagactcat cttttttatc
    26161 gtgtgaggca tgaaaacatc cttcatttca gcgcgtgtat aaaacgcgcc cactgaactg
    26221 cactgcccaa cattgagcgg gcagcgtaaa aaccaataca gaattgatac ctggatgata
    26281 tcaaatatcc agtcttaaat tgtggccttt agcggaaaaa tgaaaacata tgctacagaa
    26341 tggcgcttat cacgccattc ttcgcggtta attcagtaat ttttcagaat tatgcccggt
    26401 caacgctaaa ggcgatgact tcagccagtg tctccgcgcc cagcgccaac atcaccagac
    26461 gatcaacgcc taatgccacg ccggaacagt caggcatacc gactttcaag gcttcaatca
    26521 gattctggtc aatggggtgc tgcggcaaac cgcgcgccgc gcgcttacgg ttatcttgtt
    26581 caaagcgttg ttgctgctca cgggcatccg tcaattcatg gaaaccattc gccagctcaa
    26641 tacctttata ataaacctca aagcgttcag cgacccgatg atcttcggta ctgatttgcg
    26701 ccagtgatgc ctggctggct ggaaagtggt acacaaaggt cggtttttct ttgccaatat
    26761 ttggctctac gccaaaggta aacagcaatt gtagaagcgt gtcgcggtct tcttcagtat
    26821 cggcaacgtt gctcaaatcc agtttcgctg ccacttcccg cagttgcgtt ttgtcggcag
    26881 agagtgggtc aatttccaga taacgcaaga aagcttgttg ataagaaagg ctttctgctg
    26941 ccggacagtc cagcacctgt tgcaagagat cgtccacctc gttcatcaac cggtacatat
    27001 catagtgcgg tcgataccac tcaagcatag tgaactcagg gttgtgataa cgccccatct
    27061 cttcattacg gaagctgcgg cacagctgga ataccggccc acaaccagca accagcaggc
    27121 gtttcatatg gtattccggg ctggtcatta accagagatt catcccctgc gaatgcccgg
    27181 ggcctacgaa acgtgtctca aacgggacca aatgaatatc ggttaccgtc gcctggctca
    27241 tacaaggcgt ctccacctcc agcactccac gatcggcaaa gaaacgacgg atctccgcca
    27301 taatcgccgc gcgttttaat aagttaggaa tggatgcgct cggctgccag gatgccgttt
    27361 cgctcatagt taaatctcca gtttttgaca agggcacgaa gtctactcgc aacgcgacgg
    27421 cgagacaaat tttacgcagg aatcaaacaa gggtgggcag tgactaaaaa aagcacgatc
    27481 tgatggttta gtaattaaat taatcatctt cagtgataat ttagccctct tgcgcactaa
    27541 aaaaatcgat ctcgtcaaat ttcagactta tccatcagac tatactgttg tacctataaa
    27601 ggagcagtgg aatagcgttc gcagaccgta actttcaggc acttaccctg aagtacgggg
    27661 ctgtgggata aaaacaatct ggaggaatgt cgtgcaaacc tttcaagccg atcttgccat
    27721 tgtaggcgcc ggtggcgcgg gattacgtgc tgcaattgct gccgcgcagg caaatccaaa
    27781 tgcaaaaatc gcactaatct caaaagtata cccgatgcgt agccataccg ttgctgcaga
    27841 agggggctcc gccgctgtcg cgcaggatca tgacagcttc gaatatcact ttcacgatac
    27901 agtagcgggt ggcgactggt tgtgtgagca ggatgtcgtg gattatttcg tccaccactg
    27961 ccccaccgaa atgacccaac tggaactgtg gggatgccca tggagccgtc gcccggatgg
    28021 tagcgtcaac gtacgtcgct tcggcggcat gaaaatcgag cgcacctggt tcgccgccga
    28081 taagaccggc ttccatatgc tgcacacgct gttccagacc tctctgcaat tcccgcagat
    28141 ccagcgtttt gacgaacatt tcgtactgga tattctggtt gatgatggtc atgttcgcgg
    28201 cctggtagca atgaacatga tggaaggcac gctggtgcag atccgtgcta acgcggtcgt
    28261 tatggctacc ggcggtgcgg gtcgcgttta tcgttacaat accaatggcg gcatcgttac
    28321 cggtgacggt atgggtatgg cgctaagcca cggcgttccg ctgcgtgaca tggaattcgt
    28381 tcagtatcac ccaaccggtc tgccaggttc cggtatcctg atgaccgaag gctgccgcgg
    28441 tgaaggcggt attctggtca acaaaaatgg ctaccgttat ctgcaagatt acggcatggg
    28501 cccggaaact ccgctgggcg agccgaaaaa caaatatatg gaactgggtc cacgcgacaa
    28561 agtttctcag gccttctggc acgaatggcg taaaggcaac actatctcca cgccgcgtgg
    28621 cgatgtggtt tatctcgact tgcgtcacct cggcgagaaa aaactgcatg aacgtctgcc
    28681 gttcatctgc gaactggcga aagcgtacgt tggcgtcgat ccggttaaag aaccgattcc
    28741 ggtacgtccg accgcacact acaccatggg cggtatcgaa accgatcaga actgtgaaac
    28801 ccgcattaaa ggtctgttcg ccgtgggtga atgttcctct gttggtctgc acggtgcaaa
    28861 ccgtctgggt tctaactccc tggcggaact ggtggtcttc ggccgtctgg ccggtgaaca
    28921 agcgacagag cgtgcagcaa ctgccggtaa tggcaacgaa gcggtaattg aagcgcaggc
    28981 agctggcgtt gaacaacgtt tgaaagatct ggttaaccag gatggcggcg aaaactgggc
    29041 gaagatccgc gacgaaatgg gcctggctat ggaagaaggc tgcggtatct accgtacgcc
    29101 ggaactgatg cagaaaacca tcgacaagct ggcagagctg caggaacgct tcaaacgcgt
    29161 gcgcatcacc gacacttcca gcgtgttcaa caccgacctg ctctacacca ttgaactggg
    29221 ccacggtctg aacgttgctg aatgtatggc gcactccgca atggcacgta aagagtcccg
    29281 cggcgcacac cagcgtctgg acgaaggttg caccgagcgt gacgacgtca acttcctcaa
    29341 acacaccctc gccttccgcg atgctgatgg cacgactcgc ctggagtaca gcgacgtgaa
    29401 gattactacg ctgccgccag ctaaacgcgt ttacggtggc gaagcggatg cagccgataa
    29461 ggcggaagca gccaataaga aggagaaggc gaatggctga gatgaaaaac ctgaaaattg
    29521 aggtggtgcg ctataacccg gaagtcgata ccgcaccgca tagcgcattc tatgaagtgc
    29581 cttatgacgc aactacctca ttactggatg cgctgggcta catcaaagac aacctggcac
    29641 cggacctgag ctaccgctgg tcctgccgta tggcgatttg tggttcctgc ggcatgatgg
    29701 ttaacaacgt gccaaaactg gcatgtaaaa ccttcctgcg tgattacacc gacggtatga
    29761 aggttgaagc gttagctaac ttcccgattg aacgcgatct ggtggtcgat atgacccact
    29821 tcatcgaaag tctggaagcg atcaaaccgt acatcatcgg caactcccgc accgcggatc
    29881 agggtactaa catccagacc ccggcgcaga tggccaagta tcaccagttc tccggttgca
    29941 tcaactgtgg tctgtgctac gccgcgtgcc cgcagtttgg cctgaaccca gagttcatcg
    30001 gtccggctgc cattacgctg gcgcatcgtt ataacgaaga tagccgcgac cacggtaaga
    30061 aggagcgtat ggcgcagttg aacagccaga acggcgtatg gagctgtact ttcgtgggct
    30121 actgctccga agtctgcccg aaacacgtcg atccggctgc ggccattcag cagggcaaag
    30181 tagaaagttc gaaagacttt cttatcgcga ccctgaaacc acgctaagga gtgcaacatg
    30241 acgactaaac gtaaaccgta tgtacggcca atgacgtcca cctggtggaa aaaattgccg
    30301 ttttatcgct tctacatgct gcgcgaaggc acggcggttc cggctgtgtg gttcagcatt
    30361 gaactgattt tcgggctgtt tgccctgaaa aatggcccgg aagcctgggc gggattcgtc
    30421 gactttttac aaaacccggt tatcgtgatc attaacctga tcactctggc ggcagccctg
    30481 ctgcacacca aaacctggtt tgagctggca ccaaaagcgg ccaatatcat tgtaaaagac
    30541 gaaaaaatgg gaccagagcc aattatcaaa agcctctggg cggtaactgt ggttgccacc
    30601 atcgtaatcc tgtttgttgc cctgtactgg taaggagcct gagatgatta atccaaatcc
    30661 aaagcgttct gacgaaccgg tattctgggg cctcttcggg gccggtggta tgtggagcgc
    30721 aattattgcg ccggtgatga tcctgctggt tggtattctg ctgccactgg ggctgtttcc
    30781 gggtgacgca ctgagctacg agcgcgttct ggcgttcgcg cagagcttca ttggtcgcgt
    30841 attcctgttc ctgatgatcg ttctgccgct gtggtgtggt ttacaccgta tgcaccacgc
    30901 gatgcacgat ctgaaaatcc acgtacctgc gggcaaatgg gttttctacg gtctggctgc
    30961 tatcctgaca gttgtcacgc tgattggtgt cgttacaatc taacgcatcg ccaatgtaaa
    31021 tccggcccgc ctatggcggg ccgttttgta tggaaaccag accctatgtt caaaatgacg
    31081 ctctgcgcct tattaattac cgcctcttgc tccacatttg ctgcccctca acaaatcaac
    31141 gatattgtgc atcgcacaat taccccgctt atagagcaac aaaagatccc cggtatagcg
    31201 gtggcggtaa tttatcaggg taaaccttat tactttacct ggggctatgc ggacatcgcc
    31261 aaaaagcagc ccgtcacaca gcaaacgttg tttgagttag gttcggtcag caaaacattt
    31321 acgggcgtgc ttggtggcga cgctattgct cgaggggaaa tcaagttaag cgatcccgcg
    31381 acaaaatact ggcctgaact taccgctaaa caatggaatg ggatcacact attacatctc
    31441 gcgacctaca ccgctggcgg cctgccattg caggtgccgg atgacgttaa atcctcaagc
    31501 gacttgctgc gcttctatca aaactggcaa cctgcatggg ctccaggaac acaacgtctg
    31561 tatgccaacg ccagtattgg gttgttcggt gcactggcgg tgaaaccatc cggtttgagc
    31621 tttgaacagg cgatgcaaac tcgtgttttc cagccactca aactcactca tacgtggatt
    31681 aatgtgccgt ccgcagaaga aaagaattac gcctggggat atcgcgaagg taaggcagtg
    31741 catgtttcgc caggggcgtt agatgctgag gcttatggtg tgaagtcgac cattgaagat
    31801 atggcccgct gggtgcaaag caatttaaaa ccccttgata tcactgagaa aacgcttcaa
    31861 caagggatac aactggcaca atctcgctac tggcaaaccg gcgatatgta tcagggcctg
    31921 ggctgggaaa tgctggactg gccggtcaat cctgacatca tcattaacgg cagtgacaat
    31981 aaaattgcac tggcagcacg ccccgtaaaa gcgattacgc ccccaactcc tgcagtacgc
    32041 gcatcatggg tacataaaac gggggcgacc ggcggatttg gtagctatgt cgcgtttatt
    32101 ccagaaaaag agctgggtat cgtgatgctg gctaacaaaa actatcccaa tccagcgaga
    32161 gtcgccgccg cctgtcagat tctcaacgct ctacagtaaa atttcatcgg gtccgaattt
    32221 ttggaccttt tctccgcttt tccttgctgt catctacact tagaaaaaaa ccagtaagga
    32281 aacatttatg cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc
    32341 ctgcagttct cctacgccgc cccgtggcgt gaccgtagtg aataatttcg atgctaaacg
    32401 ctatctcggt acctggtatg agattgcccg ttttgatcac cgttttgaac gtggactgga
    32461 aaaagtcacc gcaacataca gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg
    32521 ctataaccct gacagagaaa tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc
    32581 accaactcgc gctgcgctga aagtgtcatt ctttggtcct ttctatggcg gttataacgt
    32641 tattgcactc gatcgggaat accgccatgc gctggtttgc ggtccggacc gcgactacct
    32701 gtggatactc tcccgcacgc caaccatttc tgacgaagtg aaacaggaga tgctggcagt
    32761 cgcgacccgg gaagggtttg atgtcagtaa gtttatttgg gtacagcagc ccggtagtta
    32821 gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc agactcgcca
    32881 ggcgcatcgg gttagcggac tcgccgagca gcacaatgcc ggtgatggcc gcgccgactg
    32941 cgccaatacc cgtccacacg gcataagccg tccctactgg taacgatttc atcgcccagg
    33001 caagtagcgc catactgaca atcatcgccg tcacagtaat aacacttggc gtcaaacgac
    33061 taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcagg ccagcaataa
    33121 ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg gtgaaaagag
    33181 gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca ggtggcaggg
    33241 gaatttcaac tattaccttt gtgactatcg caaaaaaaca aacggcacga cacagctgtc
    33301 gtaccgttat tgcttactgc tgcgctttcg ttgctgcgcc agaaatcgcg ttaccgccat
    33361 cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaagc agttaatact gttgaaagca
    33421 ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa
    33481 tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa aatgatggaa
    33541 atctgatgat ttttcggagg agaagaaaaa ttagctggca gcgcgggaga tggagttgcc
    33601 gagatgttta atgtcttcac caaaaccgcg agcggtgtta cagcccgtga gcagcgcact
    33661 ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata agtgatggtg
    33721 cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt caccagagcg
    33781 ggtatccact ttgatgactt cgccgatctg tacgaacagc ggaactttaa ccacagcgcc
    33841 agtagacagg gtagctggtt tgccgccagt acctgcggta tcacctttca ggcccggatc
    33901 ggtatcaacg atttccagct caacgaagtt cggcggagta acggagatcg gctgaccatt
    33961 ccacagagtt acgatacact ctgcctgatc cagcaaccat ttggcgttgt cgccaattgc
    34021 ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc agaactcacc
    34081 gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcaccttcag cggaatcagt
    34141 agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt taacgcgagc
    34201 aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt aaggttcgcc
    34261 gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg ccataaggcc
    34321 ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta accctaaata
    34381 ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat cctgatgaac
    34441 ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccgggcgc agcgccaaaa
    34501 agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa ggcaatccga
    34561 acgatccgct tttgcgtcag gtacttacct cgcaagatga gtttgttgtc gcgcccggat
    34621 tctccaccga cccgctggaa gaacagcaca gcgtagtgcc aggtttgttg cataaatacc
    34681 acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc tattgcttcc
    34741 gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag actgcacttg
    34801 agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc gatccgctga
    34861 tggcgaaaga tcacgagctg gactggttgc tcacacaact ggaagccatc ccgcatatca
    34921 aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgcatc acagatgcgc
    34981 tggttgaacg cttttctcat tctacgctgc aaatcttgct ggtgaatcac attaaccatg
    35041 ccaatgaggt agatgaaaca ttccgtcagg cgatggctaa gttgcgccgg gtaggcgtta
    35101 ctttgctcaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcgcaa acgctggcaa
    35161 acctgagtaa tgcgttgttc gatgcgggcg tgatgccgta ttacctgcat gtgctcgata
    35221 aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag attatgcgtg
    35281 agttgctgac actggtgtcg ggatatctgg tgcctaaact ggcgcgagaa attggcggcg
    35341 aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt aaacagggaa
    35401 gtttaatctt tgcacgttgc ataaccttta attattcata tacattgctg ttattacttt
    35461 ttatataacc aacaccaata atta
//
LOCUS       DAZUCC010000021        35111 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZUCC010000021 DAZUCC010000000
VERSION     DAZUCC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.021"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            129..632
                     /gene="dps"
                     /locus_tag="ACHY09_000945"
                     /gene_synonym="pexB"
     CDS             129..632
                     /gene="dps"
                     /locus_tag="ACHY09_000945"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFS3883949.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1036..1782
                     /gene="glnH"
                     /locus_tag="ACHY09_000946"
     CDS             1036..1782
                     /gene="glnH"
                     /locus_tag="ACHY09_000946"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFS3883950.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1921..2580
                     /gene="glnP"
                     /locus_tag="ACHY09_000947"
     CDS             1921..2580
                     /gene="glnP"
                     /locus_tag="ACHY09_000947"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFS3883951.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2577..3299
                     /gene="glnQ"
                     /locus_tag="ACHY09_000948"
     CDS             2577..3299
                     /gene="glnQ"
                     /locus_tag="ACHY09_000948"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFS3883952.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3416..5641
                     /gene="ybiO"
                     /locus_tag="ACHY09_000949"
     CDS             3416..5641
                     /gene="ybiO"
                     /locus_tag="ACHY09_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFS3883953.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(5638..6564)
                     /gene="rlmF"
                     /locus_tag="ACHY09_000950"
     CDS             complement(5638..6564)
                     /gene="rlmF"
                     /locus_tag="ACHY09_000950"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFS3883954.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6840..7100
                     /gene="mcbA"
                     /locus_tag="ACHY09_000951"
     CDS             6840..7100
                     /gene="mcbA"
                     /locus_tag="ACHY09_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFS3883955.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            7365..9647
                     /gene="fiu"
                     /locus_tag="ACHY09_000952"
     CDS             7365..9647
                     /gene="fiu"
                     /locus_tag="ACHY09_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFS3883956.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIKNEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9689..10366
                     /gene="ybiX"
                     /locus_tag="ACHY09_000953"
     CDS             9689..10366
                     /gene="ybiX"
                     /locus_tag="ACHY09_000953"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFS3883957.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10440..10706
                     /gene="ybiI"
                     /locus_tag="ACHY09_000954"
     CDS             10440..10706
                     /gene="ybiI"
                     /locus_tag="ACHY09_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFS3883958.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            10971..11231
                     /gene="ybiJ"
                     /locus_tag="ACHY09_000955"
     CDS             10971..11231
                     /gene="ybiJ"
                     /locus_tag="ACHY09_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFS3883959.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(11371..12456)
                     /gene="hcxB"
                     /locus_tag="ACHY09_000956"
     CDS             complement(11371..12456)
                     /gene="hcxB"
                     /locus_tag="ACHY09_000956"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFS3883960.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAVVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGMPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQKTLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARKVGMPEETLQAFCQQLAS"
     gene            complement(12597..13559)
                     /gene="ybiB"
                     /locus_tag="ACHY09_000957"
     CDS             complement(12597..13559)
                     /gene="ybiB"
                     /locus_tag="ACHY09_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFS3883961.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIKRCLAGSEPIPESLKIQMACCLMATGEAATINDGLARINQAF"
     gene            complement(13587..15737)
                     /gene="dinG"
                     /locus_tag="ACHY09_000958"
     CDS             complement(13587..15737)
                     /gene="dinG"
                     /locus_tag="ACHY09_000958"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFS3883962.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            15841..16041
                     /locus_tag="ACHY09_000959"
                     /pseudo
     CDS             15841..16041
                     /locus_tag="ACHY09_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            complement(16276..17637)
                     /gene="rhlE"
                     /locus_tag="ACHY09_000960"
     CDS             complement(16276..17637)
                     /gene="rhlE"
                     /locus_tag="ACHY09_000960"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFS3883963.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            17866..18537
                     /gene="cecR"
                     /locus_tag="ACHY09_000961"
     CDS             17866..18537
                     /gene="cecR"
                     /locus_tag="ACHY09_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFS3883964.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            18540..19535
                     /gene="hlyD"
                     /locus_tag="ACHY09_000962"
     CDS             18540..19535
                     /gene="hlyD"
                     /locus_tag="ACHY09_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFS3883965.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            19528..21264
                     /gene="ybhF"
                     /locus_tag="ACHY09_000963"
     CDS             19528..21264
                     /gene="ybhF"
                     /locus_tag="ACHY09_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS3883966.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            21257..22390
                     /gene="ybhS"
                     /locus_tag="ACHY09_000964"
     CDS             21257..22390
                     /gene="ybhS"
                     /locus_tag="ACHY09_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3883967.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            22401..23507
                     /gene="ybhR"
                     /locus_tag="ACHY09_000965"
     CDS             22401..23507
                     /gene="ybhR"
                     /locus_tag="ACHY09_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3883968.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(23469..23879)
                     /gene="ybhQ"
                     /locus_tag="ACHY09_000966"
     CDS             complement(23469..23879)
                     /gene="ybhQ"
                     /locus_tag="ACHY09_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFS3883969.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            24012..24773
                     /gene="ybhP"
                     /locus_tag="ACHY09_000967"
     CDS             24012..24773
                     /gene="ybhP"
                     /locus_tag="ACHY09_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS3883970.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKMQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            24770..26011
                     /gene="clsB"
                     /locus_tag="ACHY09_000968"
     CDS             24770..26011
                     /gene="clsB"
                     /locus_tag="ACHY09_000968"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFS3883971.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAMFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            26011..26967
                     /gene="ybhN"
                     /locus_tag="ACHY09_000969"
     CDS             26011..26967
                     /gene="ybhN"
                     /locus_tag="ACHY09_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFS3883972.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(27003..27392)
                     /locus_tag="ACHY09_000970"
     CDS             complement(27003..27392)
                     /locus_tag="ACHY09_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3883973.1"
                     /translation="MESYSQSSNKLDFQHEARILNGIWLITALGLVTTAGLALVIIRN
                     AALMSERPIWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVSCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(27598..28302)
                     /gene="ybhL"
                     /locus_tag="ACHY09_000971"
     CDS             complement(27598..28302)
                     /gene="ybhL"
                     /locus_tag="ACHY09_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS3883974.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(28439..28891)
                     /gene="moaE"
                     /locus_tag="ACHY09_000972"
     CDS             complement(28439..28891)
                     /gene="moaE"
                     /locus_tag="ACHY09_000972"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFS3883975.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(28893..29138)
                     /gene="moaD"
                     /locus_tag="ACHY09_000973"
     CDS             complement(28893..29138)
                     /gene="moaD"
                     /locus_tag="ACHY09_000973"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFS3883976.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSLDHPLTDGDEVAFFPPVTGG"
     gene            complement(29131..29616)
                     /gene="moaC"
                     /locus_tag="ACHY09_000974"
     CDS             complement(29131..29616)
                     /gene="moaC"
                     /locus_tag="ACHY09_000974"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFS3883977.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(29619..30131)
                     /gene="moaB"
                     /locus_tag="ACHY09_000975"
     CDS             complement(29619..30131)
                     /gene="moaB"
                     /locus_tag="ACHY09_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFS3883978.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(30153..31142)
                     /gene="moaA"
                     /locus_tag="ACHY09_000976"
     CDS             complement(30153..31142)
                     /gene="moaA"
                     /locus_tag="ACHY09_000976"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFS3883979.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(31130..31274)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            31539..32447
                     /gene="yvcK"
                     /locus_tag="ACHY09_000977"
     CDS             31539..32447
                     /gene="yvcK"
                     /locus_tag="ACHY09_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS3883980.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(32639..34660)
                     /gene="uvrB"
                     /locus_tag="ACHY09_000978"
     CDS             complement(32639..34660)
                     /gene="uvrB"
                     /locus_tag="ACHY09_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS3883981.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         8315 a         9332 c         9056 g         8408 t
ORIGIN      
        1 ggttttgttt tttcacgctt gttaccacta ttagtgtgat aggaacagcc agaatagcgg
       61 aacacatagc tggtgctata cttagtctcg ttaattactg ggacataaca tcaagaggat
      121 atgaaattat gagtaccgct aaattagtta aatcaaaagc gaccaatctg ctttataccc
      181 gcaacgatgt ctctgatagc gagaaaaaag caacagtaga gttgctgaat cgccaggtta
      241 tccagtttat tgatctttct ttgattacca aacaagcgca ctggaacatg cgcggcgcta
      301 acttcattgc cgtacatgaa atgctggatg gcttccgcac tgcactgatc gatcatctgg
      361 ataccatggc agaacgtgca gtgcagctgg gcggtgtagc tctggggacc actcaagtta
      421 ttaacagcaa aactccgctg aaaagttacc cgctggacat ccacaacgtt caggatcacc
      481 tgaaagagct agctgaccgt tacgcaatcg tcgctaatga cgtacgcaaa gcgattggcg
      541 aagcgaaaga tgacgacacc gcagatatcc tgaccgccgc gtctcgcgac ctggataaat
      601 tcctgtggtt tatcgaatct aacatcgaat aaatccatcg ctgatggtgc agaactttag
      661 tacacgataa aggcggcttc ctgacaggag gccgttttgt tttgtagccc gcctcaacgc
      721 acttatttag tgcatccatc tgctatcttc agctgattaa gtaaattttt tgtatccaca
      781 tcatcacaca atcgttacat aaagattgtt ttttcatcag gttttacgct aaataatcac
      841 tgtgttgagt gcacaatttt agcgcaccag tttggtgccc cagaatggtg catcttcagg
      901 gtattgccct ataaatcgtg catcacgttt ttgccgcatc tcgaaaaatc aatgagttgc
      961 aaaactggca cgattttttc atatatgtga atgtcacgca ggggatcgtc ccgtggatag
     1021 aaaaaaggaa atgctatgaa gtctgtatta aaagtttcac tggctgcact gaccctggct
     1081 tttgcggttt cttctcatgc cgcggataaa aaattagttg tcgcgacgga taccgccttc
     1141 gttccgtttg aatttaaaca gggcgataaa tacgtgggct ttgacgttga tctgtgggct
     1201 gccatcgcta aagaactgaa gctggattac gaactgaagc cgatggattt cagtgggatc
     1261 attccggcac tgcaaaccaa aaacgtcgat ctggcgctgg cgggcattac catcaccgac
     1321 gagcgtaaaa aagcgatcga tttctctgac ggctactaca aaagcggcct gttagtgatg
     1381 gtgaaagcca acaataacga tgtgaaaagc gtgaaagatc tcgacgggaa agtggttgct
     1441 gtgaagagcg gcactggctc cgttgattac gcgaaagcaa acatcaaaac taaagatctg
     1501 cgtcagttcc cgaacatcga taacgcctat atggaactgg gcaccaaccg cgcagacgcc
     1561 gttctgcacg atacgccaaa cattctgtac ttcatcaaaa ccgccggtaa cggtcagttc
     1621 aaagcggtag gtgactctct ggaagcacag caatacggta ttgcgttccc gaaaggtagc
     1681 gacgagctgc gtgataaagt caacggcgcg ttgaaaaccc tgcgcgagaa cggaacttac
     1741 aacgaaatct acaaaaaatg gttcggtact gaaccgaaat aataacgcta cgcctgtaag
     1801 gcgcactggc agttccctct cccctctggg gagaggatta gggtgagggg cgcaaacccg
     1861 ctccggggcc attaattacc ctgaatttga ttatttacac cacggtaaca ggaacaacat
     1921 atgcagtttg actggagtgc catctggcct gccattccgc ttctgattga aggtgccaaa
     1981 atgaccctgt ggatctcggt cctcggtctg gctggcggcc tggtgatcgg attgctggca
     2041 ggttttgcac gcaccttcgg tggttggata gccaaccacg tcgcgctggt ctttattgaa
     2101 gtgatccgcg gcacacctat cgtcgtccag gtgatgttta tctatttcgc cctgccgatg
     2161 gcatttaacg acttacgcat cgacccattt actgcggcgg tggtcaccat catgatcaac
     2221 tccggcgcat acattgcgga aatcacgcgt ggtgcggtgc tgtctattca caaaggtttt
     2281 cgtgaagcag gactggcgct cggtctttca cgttgggaaa ccattcgcta cgtcatttta
     2341 ccgctggcac tgcgtcgtat gctgccgccg ctgggtaacc agtggatcat cagcattaaa
     2401 gacacctcgc tgtttattgt tatcggcgtg gcggaactga cccgtcaggg gcaagaaatt
     2461 attgccggta acttccgcgc ccttgagatc tggagcgccg tggcggtgtt ctatctgatt
     2521 attaccctgg tgctgagctt tattctgcgt cgtctggaaa gaaggatgaa aatcctgtga
     2581 ttgaatttaa aaacgtctcc aagcattttg gtccaaccca ggtgctgcac aatatcgatt
     2641 tgaacattgc tcagggcgaa gtcgtggtga ttatcgggcc gtccggttcc ggtaaatcga
     2701 ccctgctgcg ctgcatcaac aaactggaag aaatcacttc cggcgatctg attgtcgatg
     2761 gcctgaaggt taacgatccg aaagttgacg agcgcctgat tcgccaggaa gcaggtatgg
     2821 tgttccagca gttttacctc ttcccgcatc tgacggcgct ggaaaacgtc atgtttggtc
     2881 cgctacgcgt gcgtggcgcg aacaaagaag aggcggaaaa actggcgcgt gagctgctgg
     2941 cgaaagtcgg tctggcagaa cgtgcacatc actacccttc cgaactttct ggtggtcaac
     3001 agcagcgtgt ggcgatcgca cgcgcgctgg cggtgaagcc gaaaatgatg ctgtttgatg
     3061 aaccgacttc cgctcttgac ccggaactgc gccatgaagt gctgaaggtt atgcaggatc
     3121 tggctgaaga agggatgacg atggtgatcg tgacacacga aatcggtttt gccgagaaag
     3181 tagcttcgcg cctgatcttt atcgacaaag gccggattgc ggaagatggc gatccgcagg
     3241 tgttgatcaa gaacccaccg agccagcgct tgcaggaatt tttgcagcac gtctcttaac
     3301 ttctcagcct ccctttcctc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
     3361 ccctcctcca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
     3421 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
     3481 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
     3541 cgaacaaaaa aaagcggcct atggtgcact ggcggatgtg ctggataatg acacctcacg
     3601 taaagagttg atcgaccagt tgcgcaccgt cgccgcgacg ccccctgcgg aacccgtacc
     3661 gaagatcgtg ccgccgacgc tggttgaaga gcaaaccgtg ctgcaaaaag tcaccgaagt
     3721 cagccgccat tatggtgaag ccctttccgc ccgcttcgga caactttatc gcaatatcac
     3781 cggctccccg cataagccgt ttaatccaca aaccttcagc aatgcgttga cccatttttc
     3841 aatgttagcg gtattagtgt ttggttttta ctggctgatt cgcctgtgcg cacttccgct
     3901 gtatcgcaaa atgggccagt gggcgcggca aaaaaatcgt gagcgcagta actggttgca
     3961 gcttccggcg atgattatcg gggcgtttat tatcgacctg ctgttactgg cactgacatt
     4021 atttgtcggt caggttttaa gcgacaacct gaatgcgggc agtcgcacca tcgctttcca
     4081 acaaagtttg tttctcaacg cctttgccct gattgaattt ttcaaagctg tactacgcct
     4141 gattttttgc ccaaatgtgg cggagctgcg cccgttcacg attcatgacg agaccgcccg
     4201 ttactggagc cgtcgcctga gctggttaag cagtttgata ggttatggcc tgattgtggc
     4261 cgtgccgatt atctctaatc aggtgaatgt acaaattggc gcgctggcga acgtcatcat
     4321 tatgctgtgc atgaccatct gggcgttgta cctgatcttt cgtaataaaa aagagattac
     4381 ccaacatttg ctcaactttg cggagcattc gctggccttt ttcagcttgt ttatccgcgc
     4441 ctttgcgctg gtgtggcact ggctggcaag cgcctatttt atcgtgctgt ttttcttttc
     4501 gttgttcgat ccgggcaaca gcctgaaatt tatgatgggt gcaacggtgc gcagcctggc
     4561 gattattggt atcgcagcat ttgtttccgg tatgttttcc cgctggctgg cgaaaaccat
     4621 caccctctcg ccacatactc agcgaaatta tccggagctg caaaaacggc tgaatggctg
     4681 gctttcggcg gcgctgaaaa cggcgcgtat tctgaccgtc tgcgtggcgg taatgctgct
     4741 gttgagcgca tggggattat tcgatttctg gaactggctg caaaacggtg cggggcagaa
     4801 aaccgtagat atcctgatcc gtatcgcact cattcttttc ttctcggcgg ttggctggac
     4861 ggtgctcgcc agtttgatcg aaaaccggct ggcttcggat attcatggcc gcccactacc
     4921 cagcgcccgt acgcgtaccc tgctgacgct gttccgtaac gcgctggcgg tgattatcag
     4981 taccatcacc atcatgattg tgttgtcgga aatcggcgtt aatatcgcgc cattgctggc
     5041 aggtgccggg gcattaggtc tggctatctc ttttggttcg caaacgctgg tgaaagatat
     5101 tatcaccggg gtatttattc agtttgaaaa cggcatgaac actggagatt tggtgactat
     5161 cgggccgttg accggcactg tggaacggat gtcgattcgc tccgtgggcg tgcgccagga
     5221 taccggggcg tatcacatca ttccgtggtc ttcgataacc acctttgcta acttcgtccg
     5281 cggtattggt tcggtagtgg caaactacga tgttgatcgc catgaagatg ctgataaagc
     5341 caatcaggca ctgaaagatg cggtagcaga attgatggaa aacgaagaaa ttcgcgggct
     5401 ggttattggt gaaccgaact ttgccgggat tgtcggctta agcaataccg cgtttacact
     5461 gcgtgtttcg ttcaccacgc tgccactcaa acagtggacg gtccgctttg ccctcgacag
     5521 ccaggtgaaa aaacatttcg acctggcggg tgttcgcgcg ccggtgcaga cttatcaggt
     5581 gctgccagct ccgggcgcgg ccccggctga accgttgccg ccgggggagc caacgcttta
     5641 acgctggcga ttgacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc aggctataaa
     5701 gcggctctgc ttttgcccct gggccatctc tttttttacc accttcaccg cacctacgtc
     5761 cgtcaaagca cgatacaacg gcggtaagtt ttcaccacga gataccagcg aggtaaacca
     5821 catcacctgc ttcgcgaagc ctttgctctc ttcaatcatc tttttgataa aagcgacctc
     5881 accgccttca caccacaact cctgttgctg accaccaaag ttcagtgcat cgtctttgtt
     5941 cagccccagg ttacggcgtt tacgttcact acctgcccgt gccgcagcgg cggaatcgtg
     6001 gaatggcggg ttacacaagg tcgcatcgta ttgctcgttt ttatggatga tgccgttaaa
     6061 aatcgcccca ctctcttttt gccgacgcag acgaatggcg cggttaagcc ccggattagc
     6121 actgatgatc gcctgcgcac tgcttaacgc ctggctgctg gtttcactac cggtaaaacg
     6181 ccagccatat tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa caccgatatc
     6241 cagaatgctg gcattagctg gaattgttcc gcttgcttca gccagtaaat cggcaaggtg
     6301 atgaatataa tccgcccggc ccggtactgg cgggcagaga aaaccgtccg ggatatccca
     6361 gttcgctacg gcgtaaaaat gggccagcaa cgccttattg agcgccttca ccgccagcgg
     6421 attggcaaag tctacgcttt gctccccggc gggtgtaagc gtgaggaatt gcctgagttc
     6481 aggattgacc tgacaaagcg tggcgagatc gtagcggctg tgatgacggt tgcgcggatg
     6541 caaccccggt ttctgggcgg acatagcatt ctcctttaag cagcggcgta agatacccgc
     6601 ataacgcgac agggtaaata tctggctgcg tttatcattt ctaaaaacct tcacatggct
     6661 atggtttatg gtaattcacc ataaaaatca tcatcatgca tgtcattgat ttatcttatt
     6721 ttgtaaaaat atattgttca aaaaataagc gcatctctgt caattttttg cgtcattttc
     6781 atcgcgcagg cgacctacac ttatctggca ctacccgttg tatttatgag gatgtcatta
     6841 tgaaaaagtg cctcacacta ctgattgcca ccgtcctgag cggaatctct ctcacggctt
     6901 atgccgcgca accgatgagt aacctggaca gtggtcaact acggcccgcc ggtaccgttt
     6961 cggcaactgg cgcatcaaac ctaagcgatc tggaggataa actggcagaa aaagcgcgcg
     7021 aacaaggcgc gaaaggttat gtcattaatt ccgcaggcgg aaatgaccag atgttcggta
     7081 ctgcaatcat ctacaaataa ccccaccaac gtaactgcat tcgcctctgg atgcagtttt
     7141 cgtccaattt gtaagcaaaa aacatcaaat atgaacgcaa tgtaaataaa tgtatttctt
     7201 tttcgcgcaa tgggtgatag aaaatcgcgc caagtgataa tgcttatcaa aattattatc
     7261 acttgcacgg acgctatcac gggattaaca gtggcatcgc atccgcagag atgctttctc
     7321 gtgacagtga aaatttcacc atataagaaa aagtcacctg caaaatggaa aacaatcgca
     7381 atttccctgc cagacaattt cattcgctca cgttctttgc cggtctttgt attggtatta
     7441 cgcctgtggc gcaggcactc gctgccgaag ggcaagctaa cgcggatgac acgctggttg
     7501 tcgaagcatc aacgccttcg ctttatgcgc cacaacaatc tgccgatccg aaattctcgc
     7561 gtccggtagc ggatactacc cgcacgatga cggtgatttc tgaacaagtg attaaagatc
     7621 agggtgcaac caaccttacc gacgcgctca aaaacgtccc cggcgtgggt gcgttttttg
     7681 cgggtgagaa cggtaactcc accactggcg acgccattta tatgcgcggt gccgatacct
     7741 ctaacagtat ttatatcgat ggcattcgcg atatcggcag cgtctcgcgc gacaccttca
     7801 ataccgagca ggtcgaagta attaaagggc cgtccggcac cgactacggg cgcagcgcgc
     7861 cgacaggctc gatcaatatg atcagcaaac agccgcgcaa tgattccggc attgacgcct
     7921 ccgccagtat tggcagcgcc tggttccgcc gcggcacgct ggacgtcaat caggtcattg
     7981 gtgataccac tgcggtgcgc ctgaatgtaa tgggcgaaaa aacgcacgat gccggacgcg
     8041 acaaagtcaa aaatgagcgt tacggcgtcg ccccttctgt cgcttttggc cttggtacag
     8101 cgaatcgttt gtatcttaat tatctgcatg tcacccagca caacacgcca gacggcggca
     8161 ttccgactat cggtttaccg ggttattctg ccccatctgc gggaacagcg gctctgaatc
     8221 attccggaaa agttgatact cataactttt acggcacgga ttccgattac gacgattcga
     8281 ccaccgacac cgctaccatg cgttttgagc acgacatcaa cgataacacc accattcgca
     8341 atactacccg ttggtcgcgc gtgaagcagg attacctgat gacggcgatt atgggcgggg
     8401 cgtcgaatat tacccagccc accagcgatg tgaatagctg gacctggtca cgcacggcga
     8461 atactaaaga tgtgagtaat aaaattctca ccaaccagac caacctgacc tcgacgttct
     8521 ataccggcgc tatcggtcat gatgtcagta ccggcgtgga atttacccgt gaaacgcaga
     8581 ctaactacgg cgttaatccg gtgacgttac ctgcggtaaa tatttatcat cctgacagca
     8641 gcattcatcc cggcggcctg acgcgcaacg gcgcaaacgc caatggtcag acggatacct
     8701 tcgcaattta cgccttcgat acgctgcaaa tcacccgtga ttttgagctg aacggcggga
     8761 tccgtctgga taattatcat actgaatatg acagtgccac cgcctgcggc ggcagcggac
     8821 gcggtgccat cacctgccct gctggcgtgg caaaaggttc tccggtcacc accgtcgata
     8881 ccgccaagtc gggcaatctg gtgaactgga aagccggggc gctgtatcac ctgacggaaa
     8941 acggcaatgt ctatattaac tatgccgttt cccagcagcc tccgggcggc aacaacttcg
     9001 cccttgcgca gtctggcagc ggtaacagtg ccaaccgcac cgattttaaa ccgcaaaaag
     9061 ccaacaccag cgagattggc accaaatggc aggttctgga taaacgcctg ttgctcaccg
     9121 ccgcgctgtt ccgtactgat atcaaaaatg aagttgagca aaatgatgac ggaacttact
     9181 cgcaatacgg taagaaacgc gtcgaaggct atgagatatc cgtggccggg aatatcactc
     9241 ccgcgtggca ggtgattggc ggctataccc agcaaaaagc aaccatcaaa aacggcaaag
     9301 atgttgccca ggatggttcc tcatcgctgc cgtatacccc ggagcacgcc tttaccttat
     9361 ggagccagta tcaggcaacc gatgatattt ccgttggtgc gggcgcacgc tatatcggca
     9421 gtatgcataa aggttctgac ggcgcggtgg gaacgccagc gtttaccgaa ggttattggg
     9481 tcgccgacgc taaactgggg tatcgggtta atcgcaatct cgacttccag ctaaacgtct
     9541 acaacctgtt tgataccgat tacgtcgcct caatcaacaa gagcggctac cgttatcacc
     9601 cgggcgagcc aagaaccttc ttgctcacgg ccaatatgca tttctgattg agatgtgggg
     9661 cgcaggcccc actttttgga gaacttgtat gatgtaccac attcccggcg tgctatcgcc
     9721 acaggacgtc gcccgttttc gcgaacaact ggaacaagcc gaatgggtgg atggacgcgt
     9781 caccactggc gcacaaggtg cgcaagttaa gaacaatcaa caggtagaca cccgcagcac
     9841 gttatacgcc gccctgcaaa atgaggtgct gaacgcggtt aaccaacatg ctttgttctt
     9901 tgccgcggcc ttgccgcgta ccctttccac gccgctgttt aatcgctatc agaacaatga
     9961 aacctatggt tttcatgtgg atggcgcagt acgcagtcac ccacaaaacg gctggatgcg
    10021 cactgacctt tctgccacgc tgtttttaag cgatccacaa agctacgatg gcggcgaact
    10081 ggtcgttaat gacaccttcg ggcaacatcg ggtaaaactt ccggcaggcg atctggtgtt
    10141 gtatccctcc agcagcctgc attgcgtgac acccgtaacc cgcggcgtac gagtggcatc
    10201 atttatgtgg atccagtcga tgatccgcga tgataaaaag cgcgccatgc tgtttgaact
    10261 ggacaacaat attcagtcgc tgaaaagccg ctacggcgaa agtgaagaga tcctgtcgct
    10321 gctcaatctt tatcataatc tgctgcggga atggtcggag atctgacgct tgaactgccc
    10381 gagttcgtcc ctatattagc tacacttaac tgtacaagta ttgatatggg gaggacgata
    10441 tggcatccgg ttgggctaac gatgacgccg tcaacgaaca gatcaacagt acaattgaag
    10501 atgcgattgc ccgcgctcgg ggtgaaattc cgcgtggcga aagcctgtat gaatgtgaag
    10561 agtgcggagc ccccatcccg caagcccgtc gggaagccat tcctggtgtg cgcttatgca
    10621 ttcattgtca gcaggagaaa gatttacaaa aaccagctta tacaggatat aatcgcagag
    10681 gttcgaaaga cagccagtta cgttaactac aactggcggg atgtatagcg agtataattt
    10741 gtattttgcg tggcattccg tgcctttaac gctgtcgtga ctgccacgct gcataaaaca
    10801 agtccataag ttcaacaaat aaatggatta tcagtatgtt agatatcaat caatttattt
    10861 gaacaaggcg gtcaattctc ttcgatttta tctctcgaaa aaaaacgtga tactcatcac
    10921 atcgacgaaa caacgtcact tatacaaaaa tcacctgcga gagattaatt atgaaaacta
    10981 tcaatactgt tgttgctgct atggctcttt caactctgtc atttggcgtg tttgccgcgg
    11041 aaccggtaac ggcatcccag gcacagaaca tgaataaaat cggcgtggtt tctgccgatg
    11101 gcgcatccac cctcgatgcc ctggaagcga aactggctga gaaagctgcg gcagctgggg
    11161 cgagtggata cagcatcact tctgccacca acaacaataa attaagcggt actgcggtaa
    11221 tttataagta attcgtagaa cggataaggc gtttacgcta cgtccagcac aagtatttcc
    11281 ctgtctgttt gccgacagac gcatatgctc taaccctcat tgatcctatg ttacccttgt
    11341 ttgcccgtcc gccactggac gggctttttt ttagctggct aactgctgac agaaagcctg
    11401 caacgtctct tccggcatac caacctttcg cgccgcatca caaatggcct gccagcttcc
    11461 cgcatccaat ggaatcccct gctcctgccg ttcgcgacgc gtgttaactt cccactcgcc
    11521 cggtagcaaa atcggcttat cgtcatcatg cggcgaggct ttcacccact cggcaaaggc
    11581 ttcggtctgc gcgctacaat ccggcgcgcc gaacagttcc gggttgatga tgatagtggt
    11641 catgcagtta agaatggcat cgggactggt ttgtaacgtt ttctgatgcg tcgttttacc
    11701 gccggaaagc gccccaccaa gaatttcaca catcgctgca agggcgtagc ctttatgttc
    11761 ggcaaaggtc aacagcgaac ccaacggcga ctcctgcatt accgccggat tggtcgtcgg
    11821 catgccgtta acgtcaatca ggcaacctgg cggcacgggg acgcctttat gccaggcgac
    11881 gcgggttttg ccaaatgcga tggcgctggt ggcgtaatca agcaacagcg ggaaattatc
    11941 tttacgaggg aaaaccacac agaacggatt ggtgccaaag cggctgtcgc gaccgtggaa
    12001 cggcgcgacc attggaatac cgaccacgct aacaaagtgg atagagacaa accccgccgc
    12061 tgcacactgc tccgcccagt aaccgatacg gccgatatga tgcgagttat gtagcgccac
    12121 ggcagcaata ccgtgctgat gcgctttctc aatccccagc gccatcgctt cgtgtgccgc
    12181 gacctgacca aatgcgcgat cgccatcaag cgtaaccacc gcgcccgctt ctttaacgat
    12241 tttggcatgg tggttaattt gcagatgccc ctgactccag gaacgcacat agcttgggat
    12301 catgccaata ccatgtgaat catgccctgc caggtttgcc gcgattaaat gatcggcaac
    12361 taatttcgct tcttgttcct cgctacccat ctgacgaaat acagcctgaa taaaactgtg
    12421 cagcgtctga gcatcaaagc gatgaccact ttccatggtt tactcctgtt tatgttatgt
    12481 gtttgttgtt tttttatgct gcacgccggg cgcgggaagg tcaataaaga tgcaggaatt
    12541 tcatattgcg gtggggtaaa agccggatga catggctcat ccggctgaga taaaaattaa
    12601 aatgcctgat taatgcgcgc caggccgtcg ttgatagttg ccgcttcacc cgtagccatc
    12661 aggcagcaag ccatctggat tttcagcgat tcaggaatcg gttcgctgcc agcaaggcaa
    12721 cgcttaatcc actgcgccgt ggtttccgga tcttttgctt gtggcagtaa ctcgctacca
    12781 gcagtgtcct gtttttcata cagtacccgc atcccttcac ggtcaatgag attgatctgc
    12841 gggcagcgct gcggattagc atacacttca ccttcagtac catgcattag tagcgcccgc
    12901 ccgccgatat cactaaagaa cttcgcgacg cgtccaatgt attccggatg cgaaacgctg
    12961 gaaagacgca gtgcctcacc ttcggcaaat ggcgtcgcca gtttcgccag ggtatgtgca
    13021 ctgttacgca cgcccatccg ccagcgcatc gccagttgtt tttccagcgg cgggcaaaac
    13081 gcgccgacag gcataaacac cggttgatgt tcgtcgagct tcgcctgcgc ctgcccgccg
    13141 tgcaacgttg gcgtaatacc catcagttcg aaaatggttt ctgtcagcac gcgggttgga
    13201 tcttcgctaa ccccgtgaac caccacagga aaaccgagtt tgtgcaggag aatcgccaac
    13261 aacggcgtca ggttggcctg tttacgcgcg ccgttgtaac tggggatgac aatcggcatc
    13321 ggcttgtctg caggcggcgt cagcttgatg gtgtgatttt gcatggcttc gtaaaagccg
    13381 agcatctctg cttccccttc ccctttgata cgcagcgcaa tcaatacgcc gcccaactca
    13441 aggtcaggga cttcaccatt gagcatatgg gcatacagac cgcgcgcagt atcccggtct
    13501 aaatcgcgcg cgtggttttt cccgcgcccg atctctttaa tgattttgcg atagtccatt
    13561 tacgactcct tacctgactc acatcattaa cgccgccgac ggcgtgggga tttcgttttt
    13621 tctttctttt taactatacc ttcagggact tccggttgct ctatcggaaa taccggtaat
    13681 gcatccagta gtcgcttgcc ataatttttg gtcagcaagc gtttgtcgta gataaccact
    13741 tccccccagc aaccgtggct tcgaatcagt cgcccaacct gctgaatcag gttaaacgaa
    13801 gcgctcggca ggctttgcac ctcaaacgga tagcggttga ggcttttcag ccattcacct
    13861 tcggtgatca ccaccggact gtcgatgggc ggaaaagcga tcttgtggat atgcacctga
    13921 ctgagcagat cgcctttcag atcaagacct tcggcaaagg actgtaagcc caccagcacg
    13981 ctacgctcac cgttggcaac gcgtttgcgg tgcagttcaa ctaagcgata acgtggctga
    14041 tcgccctgca ccagcaacat caggcgtaaa tccgtcacat agtcgagaaa gcgctgcatc
    14101 gcacgcccgc tggcaaacag taccaacata ccgagatgtt ttttgctctc cacctgctca
    14161 cggaaaaagg ctgccatttc agcaatgtgc tgctcttcgt tgtcgatgga aggctcaaag
    14221 cgcatccggg gaataacaat tttgccctgt tcgcagtggt taaagggcga atccagcgcc
    14281 acaaagcggt cgcccgcttt ctctttcaga ccactcatct cctgtaaacg cgaaaaactg
    14341 ttcagcgaac gtaaggtcgc ggaagtgaca ataatgtgcg gaatactgcg ccacagcagc
    14401 ctttccagct gatcgctgac acgaataccc acacagtgaa accagagatg cagctgccct
    14461 tcgcgctctt cccgcgtcgc ccatttagtc accggcgcac cggaagattg cgccagagac
    14521 gccagccgcc agagtttgct ttgcgcctcg aacatcccca acgcgcgatt catctgcaaa
    14581 atcaaccgat gcagacgtac aatgtcatgg ctaccggttt tctcacttaa atcgttaaga
    14641 aataactccg ccaggccacg cagcatctcg gtgagttttg ccagccgctg gcagatctcc
    14701 agcacttcat caggcagttc gcccatcgca aaacggtgct ctgcctcctg cccggcaggc
    14761 atgtagagat tgagaatgtt gtttaacgag gcgataagct catacagctc ttcacaatgc
    14821 gcattcaaac gttcagggat cgccagcggt gggatggtct tcgggcgaaa ctgctccatg
    14881 caggtagcga ccagtttcgt gaacaagtcc agctgtagcc gataccaggg cgcggtgatt
    14941 tcggcgctca tctccagcgc atcccgcgcc acatctggca ggtgatgacc ttcgtccagc
    15001 accagcagca gatttttcgg gtcaggtagt acggcttcgc tttccatcgc cgccatcacc
    15061 agcgcatggt ttgccaccac cacttccgct tcctgaatct cccgacgagc aacaaaaaac
    15121 gggcattcac gatagtagta acagttgcgg ttgaggcagc tggctttgtc ggtacttaaa
    15181 cgacgccaga gatcgtcatc aatggcgata tccgtatgat cgcgcagacc atcccattta
    15241 taagtgtcga gatcgccctt cagcttcgca caacgttttt gctcttcctg attgttcggc
    15301 gtcagctcgt cgtcaagaaa cgccagcaga tcctgttgcg tgggttcagt actggcaagc
    15361 gctgtcagat tacgcggaca aacgtagcgc ccacgcccaa aagcagcagt gaatttaaga
    15421 tcgggaatga tctttttcag cagaggcaaa tctttactgt agatctgatc ctgcaatgcc
    15481 acgttggcgg tactcaccac cagcgttttt tgctcttcgc gggcaatggc gatgccggga
    15541 atcaaatagg agagcgtttt cccaacgccg gtgggtgctt caatcgccag atgccgccct
    15601 tcttctccgg ccagcgtttt ggcgacgtcc gcaatcatct gccgctgcgg cgcacgggga
    15661 ataaagtcgg ggatctgttc ctgaagcgcc ttataccagg cggcaatttg cgctttaagc
    15721 gcggcggtta atgccatgag aaaacctgaa atactgtata aacagccaat attgtggcat
    15781 ttttcggttt tcaggggtaa tggtttttct gtttctggaa agcggctcgc aataaccagc
    15841 aggatgatgc gtaaagcgct tcgcgctaaa ttcgaacaac atgcagaact gcgtacactc
    15901 ttgctggcaa ccgcgtccgc aaaactggtt gagcatacgc aaaatgatgc ttactgggga
    15961 gatagcggta atggtcaggg caagaatcgc ctggggtacc ttttaatggc gttgcgcgga
    16021 caattggctg cggagaagta acgcatcggg tggcttgtgg gcgttaaaac agagaaatct
    16081 ttttgccgcg ctcgcaaatg gctgacaagt gcttgttgtg aaatgccgga tgcggcgtaa
    16141 acgccttatc cggcctacaa aagcatgcaa attcaatata ttacagagtt cacgtaggcc
    16201 tgataagcgc agcgcatcag gcagttttgc gtttgttcat cagcctgatg ccgggcatcg
    16261 cccggcatca aaagattact gcgcagcggc aggtttacgt ggacggcggc gacgttgttg
    16321 ttcgcctgcc ggtttggcat cgccgaggcg gcgagacggt ttttctgcag gtttcgcact
    16381 tgcagatttt gcgccacctt ctccacggcg tggttgctgt tgaccgcgac caccaccttg
    16441 cccacgaccg ccgccacgtt gctggcgacc gttctggatc ggttcggctt tgattgacgg
    16501 gtccggctca taacccggaa tcgcaatgcg cgggatctct tttttcagca gtttttcgat
    16561 atcacgcagc agtttgtgtt catcaacaca caccagcgac aacgcttcac cggtagcagc
    16621 cgcacgaccg gtacgcccga tacggtggac ataatcttcc ggtacgtttg gcagttcata
    16681 gttgaccacg tgcggcagct cttcaatatc caggccgcgc gcagcgatgt cagttgccac
    16741 cagtacacga atatcgcccg atttaaaatc agccagcgca cgagtacgcg caccttgcga
    16801 tttattaccg tggatcgccg cactacggat gccatcttta ttaagctgtt cagccagatg
    16861 gttagcgcca tgtttggtac gggtaaacac cagcacctgc tgccagttcc ctttcccaat
    16921 catatgcgac agcaattcgc gtttgcgttt cttatcgaca aagtgaacgt gctgggtcac
    16981 ctgatcagac gcggtattgc ggcgtgccac ttcgatttcc agcgggttgt gcaacagttt
    17041 ttccgccagg gctttaatat cgtcagagaa ggtcgcggag aacaacaggt tctggcgctt
    17101 cgctggcagt tttgttaaca cgcgacggat atcgtggata aagcccatgt cgagcatgcg
    17161 gtcagcttca tcaaggacga ggatttcaac ctgatccagc ttcactgcat tttgatgttc
    17221 caggtccagc agacgtcccg gcgttgccac cagcacatca acgccgccgc gcaacttcat
    17281 catttgtggg ttaatgctga caccgccaaa caccaccagc gaacgaatgt tcagatattt
    17341 gctgtaatca cggacgtttt cgccaatctg cgccgccagt tcacgggtcg gggtaagaat
    17401 gagcgcgcgt accggacgac gccctttggc gtgcggctgg cgagtgatca ggtgttgcaa
    17461 cagcggcagc gtaaagcccg ctgttttgcc ggtaccggtc tgggcgctgg ccatcaggtc
    17521 gcggccttcc agcaccgcag ggatcgcctg ctgctgaata ggggtgggtt cacggtaacc
    17581 ctgctcggca acggcgcgca ggatatcagg gcttaaaccc aaagaatcga aagacataac
    17641 tactccgaac cgccccgacc gttaccggtg tagttttcag ggagatactg aaaaggaaaa
    17701 tgacaaaaac cacaatgtca tgagggagcg gagtgtagca gtttttgtga cgcagcgcat
    17761 aaattatccc tgtgactggc gttgctaaaa ttcttagcca tactggacaa ctctcatatg
    17821 gagtcataat cttaatcaat catttgatta agaatggact gtgcgatgaa taatcctgcc
    17881 atgacaatca agggtgaaca ggcgaagaaa cagctgattg ctgccgcact ggcgcagttt
    17941 ggtgaatatg gaatgaacgc caccacccgc gagatagccg cccaggccgg gcagaatatc
    18001 gctgccatca cctattactt cggttcgaaa gaggatttgt acctcgcctg cgcccagtgg
    18061 attgccgatt ttattggcga gcagttccgt ccacacgccg aagaagccga acgtttgttc
    18121 gcgcaaccac agcctgatcg cgcagctatc cgtgaactga tccttcgcgc ctgcaggaac
    18181 atgattaagc tgctcaccca ggatgacacc gtcaacctca gcaaatttat ctcccgtgag
    18241 cagctctctc ccacggcagc ctatcacctg gtgcacgaac aggtgattag tccgctacac
    18301 agccacctta cacgcctgat tgccgcctgg accggctgcg acgctagcga tacccgcatg
    18361 atccttcata cccatgcgtt gattggtgag attctggcgt ttcgtcttgg caaagaaact
    18421 attctgttac gtaccggctg gaccgcgttc gatgaagaaa agaccgaact gatcaaccag
    18481 acggtgacct gtcatattga cctgattttg caaggattat cgcaaaggag tttgtagtga
    18541 tgaaaaaacc tgtcgtgatc ggactggcgg tagtggtact tgccgccgtg gttgccggag
    18601 gctactggtg gtatcaaagc cgccaggata acggcctgac gctgtatggc aacgtggata
    18661 ttcgtacggt gaatcttagt ttccgtgttg gggggcgcgt tgagtcgctg gcggtggacg
    18721 aaggtgatgc gatcaaagcg ggccaggtac tgggcgaact ggatcacaag ccgtatgaga
    18781 ttgccctgat gcaggcgaaa gcgggcgttt cggtggctca ggcgcagtat gacctgatgc
    18841 ttgccgggta tcgagatgaa gaaatcgctc aggccgccgc agcggtgaaa caggcgcaag
    18901 ctgcctatga ctatgcgcag aacttctata accgccagca aggactgtgg aaaagccgca
    18961 ccatttcggc aaatgacctg gaaaatgccc gctcctcgcg cgaccaggcg caggccacgc
    19021 tgaaatcagc gcaggataaa ttgcgtcagt accgttccgg taaccgcgaa caggacatcg
    19081 cccaggcgaa agccagcctc gaacaagcgc aggcgcaact ggcgcaggcg gagctgaatt
    19141 tacaggactc aacgttgata gccccgtctg atggcacgct gttaacgcgc gcggtggagc
    19201 ctggcacggt gctcaatgaa ggtggcacag tctttaccgt ctcactgaca cgtccggtgt
    19261 gggtacgtgc ttatgttgat gaacgtaatc tcgaccaggc ccagccgggg cgcaaagtgc
    19321 tgctttatac cgacggtcgc ccggacaaac cgtatcacgg gcagattggt ttcgtttcgc
    19381 ctaccgctga atttaccccg aaaactgtcg aaacgccgga tctgcgtacc gacctcgtct
    19441 atcgcctgcg tattgtggtg accgacgccg atgatgcctt acgccaggga atgccggtga
    19501 cggtgcaatt cggtaacgag gcaggacatg aatgatgccg ttattacgct gaacggtctg
    19561 gaaaaacgct ttccgggcat ggacaagccc gccgtcgcgc cgctcgactg taccattcat
    19621 gctggttatg taacggggct ggtggggccg gatggcgcag gtaaaaccac actgatgcgc
    19681 atgctggcgg gattactgaa acccgatagc ggcagcgcca cggtgattgg ctttgatccg
    19741 atcaaaaacg acggcgcgct gcacgccgtg ctcggctata tgccgcagaa attcggcctg
    19801 tatgaagatc tgacggtgat ggagaacctc aatctgtacg cggatttgcg cagcgtcacc
    19861 ggtgaagcgc ggaagcagac ttttgctcgc ctgctggaat ttacatctct tggcccgttt
    19921 accggacgcc tggcgggcaa gctctccggc ggaatgaagc aaaaacttgg cctggcctgt
    19981 accctggtgg gcgaaccgaa agtgttgctg ctcgatgaac ccggcgtcgg cgttgaccct
    20041 atctcacgcc gcgaactgtg gcagatggtg catgagctgg caggcgaagg aatgttaatt
    20101 ctctggagca cctcgtatct cgacgaagcc gaacagtgcc gtgacgtgtt actgatgaac
    20161 gaaggcgagc tgctgtatca gggagaaccg acggccctga ctcaaactat ggccggacgc
    20221 agctttctga tgaccagccc gcacgagggc aaccgcaaac tgttgcaacg ggcattgaaa
    20281 ctgccgcagg tcagcgacgg catgattcag gggaaatcgg tacgtctgat cctcaaaaaa
    20341 gagaccacgc cagacgatat tcgccatgcc gacgggatgc cggaaatcaa catcaacgaa
    20401 actacgccgc gttttgaaga tgcgtttatt gatttgctgg gcggtgccgg aacctcggaa
    20461 tcgccgctgg gcgcaatatt acatacggta gaaggcacac ccggcgagac ggtgatcgaa
    20521 gcgaaagagc tgaccaaaaa gtttggcgat tttgccgcca ccgatcacgt caactttgcc
    20581 gtcaaacgcg gggagatttt tggtttgctg gggccaaacg gcgcgggtaa atcgaccacc
    20641 tttaagatga tgtgcggttt gctggtgccg acctctggtc aggcgctggt gctgggcatg
    20701 gatctgaaag agagttccgg taaagcgcgc caacatctcg gctatatggc gcaaaaattt
    20761 tcgctctatg gcaacctgac ggtcgaacag aatttacgct ttttctctgg tgtgtatggc
    20821 ttacgcggtc gggcgcagaa cgaaaaaatc tcccgtatga gcgaggcgtt cggcctgaaa
    20881 agtatcgcct cccacgcgac cgatgaactg ccattaggtt ttaaacagcg gctggcgctg
    20941 gcctgttcac tgatgcatga accggacatt ctgtttctcg acgaaccgac gtccggcgtt
    21001 gatcccctca cccgccgtga attttggcta catatcaaca gtatggtaga gaaaggcgtc
    21061 acggtgatgg tcaccaccca ctttatggat gaagcggaat attgcgaccg catcggcctg
    21121 gtgtaccgcg ggaaattaat cgccagcggc acaccggacg atttgaaagc gcagtcggcc
    21181 aacgatgagc aacccgatcc cacgatggag caagccttta ttcagttgat ccacgactgg
    21241 gataaggagc atagcaatga gtaatccgat cctgtcctgg cgtcgcgtgc gggcgctgtg
    21301 cgtcaaagag acgcggcaga tcgttcgcga tccgagtagc tggctgattg cggtagtgat
    21361 cccactgcta ctgctgttta tttttggata cggcattaac ctcgactcca gcaagctgcg
    21421 ggtcgggatt ttactggaac agcgtagcga agcggcgctg gatttcaccc acaccatgac
    21481 cggttcgccc tacatcgacg ccaccatcag cgataaccgt caggaactga tcgccaaaat
    21541 gcaggcgggg aaaattcgcg gtctggtggt tattccggtg gatttcgccg aacagatgga
    21601 gcgcgccaac gccaccgcac cgattcaggt gatcaccgac ggcagtgagc cgaataccgc
    21661 taactttgtg caggggtatg tcgaagggat ctggcagatc tggcaaatgc agcgagcgga
    21721 ggataacggg cagacttttg aaccgcttat tgatgtacaa acccgctact ggtttaaccc
    21781 ggcggcaata agccagcact ttattatccc cggtgcggtg accattatca tgacggtcat
    21841 cggcgcgatt ctcacctcgc tggtggtggc gcgagaatgg gaacgcggca ccatggaggc
    21901 gttactctct acagagatta cccgcacgga actgctgctg tgtaaactga tcccttatta
    21961 ctttctcggg atgctggcga tgttgctgtg tatgctggtg tcagtgttta ttctcggcgt
    22021 gccgtatcgc ggttcgctgc tgattctgtt ttttatctcc agcctgtttt tactcagtac
    22081 cctggggatg gggctgctga tttccacgat aacccgcaac cagttcaatg ccgctcaggt
    22141 cgctctgaac gcggcttttc tgccgtcgat tatgctttcc ggctttattt ttcaaatcga
    22201 cagtatgccc gcggtgatcc gcgcggtgac gtacattatt cccgctcgtt atttcgtcag
    22261 caccctgcaa agcctgttcc tcgccgggaa tattccagtg gtgctggtgg taaacgtgct
    22321 gtttttgatc gcttcggcgg tgatgtttat cggcctgacg tggctgaaaa ccaaacgtcg
    22381 gctggattag ggagaagagc atgtttcatc gcttatggac gttaatccgc aaagagttgc
    22441 agtcgctgct gcgcgaaccg caaacccgtg cgattcttat tttacccgtg ctgattcagg
    22501 tgatcctgtt cccgttcgcc gccacgctgg aagtgaccaa cgccaccatc gccatctacg
    22561 atgaagataa cggcgaacac tcggtagaac ttactcaacg ttttgcccgc gccagcgcct
    22621 ttacccatgt gctgctgttg aaaagcccgc aggagatccg cccaaccatc gacacacaaa
    22681 aggcattact cctggtgcgt ttcccggctg acttctcgcg caaactggat accttccaga
    22741 ccgcgccgtt gcagttgatc ctcgacgggc gtaactccaa cagtgcgcaa attgccgcca
    22801 actacctgca acagatcgtc aaaaattatc agcaggagct gctggaagga aaaccgaaac
    22861 ctaacaacag cgagctggtg gtacgcaact ggtataaccc gaatctcgac tacaaatggt
    22921 ttgtggtgcc gtcgctgatc gccatgatca ccactatcgg cgtaatgatc gtcacttcac
    22981 tttctgtcgc ccgcgaacgt gaacaaggta cgctcgatca gttactggtc tcaccgctca
    23041 ccacctggca gatcttcatc ggcaaggccg taccggcatt aattgtcgcc accttccagg
    23101 ccaccattgt gctggcgatt ggtatctggg cgtatcaaat ccccttcgcc ggatcgctgg
    23161 cgctgttcta ctttacgatg gtgatttacg gtttatcgct ggtgggattc ggtctgttga
    23221 tttcatcact ctgttcaaca caacagcagg cgtttatcgg cgtgtttgtc tttatgatgc
    23281 ccgccattct cctttccggt tacgtttcgc cggtggaaaa catgccagta tggctgcaaa
    23341 acctgacgtg gattaaccct attcgccact ttacggacat taccaagcag atttatttga
    23401 aagatgcgag tctggatatt gtgtggaata gtttgtggcc gctactggtg ataacggcca
    23461 cgacagggtc agcggcgtac gcgatgttta gacgtaaggt gatgtaactt cttatctttc
    23521 gccagcaaag acactaccgc cgggccagca aggattgcca gccctgcaat cgccagcaaa
    23581 aagttgtttt ccagtacgcg ggacaacagc cacagcacaa tcagcgctgc gccaaaatac
    23641 caggtcgtgg tcagctcttc cagcacgtct gccacctcac gccagcgttg ctcggggtgg
    23701 cgctgaaaca ccagcatcat cactaccgtg acacaataca gtgcgcataa tcccacgccg
    23761 acgtgaacca gagtcttact catccagccc accaccagca gcgccactac cagtaaatgc
    23821 aacataatct gccagcaact tagacccgtt gcgacacgaa cacgttgttg ccacttcatg
    23881 gcttctctcc tgaagaattt atctctactt actcagagta ccgaacttta cggaaaattc
    23941 cgtaagaccg cacctttttg tgacgccgac tacactattt caatgagata agaggcaaag
    24001 acaggaaaat tatgcccgat caaacacaac aattttcgtt caaggtgctc accatcaata
    24061 ttcacaaagg ctttaccgcg tttaaccgac gcttcatttt gccggaactt cgcgacgccg
    24121 tgcgtaccgt cagcgccgat attgtttgcc tgcaggaagt gatgggcgcg cacgaagttc
    24181 atccgctgca tgtggaaaac tggcccgata cctcgcacta cgagtttctc gccgacacca
    24241 tgtggagcga tttcgcctac ggtcgcaatg ccgtataccc ggaagggcat cacggcaacg
    24301 cggttctttc acgttatccc attgaacatt atgagaatcg cgatgtttcg gtcgatggtg
    24361 cggaaaagcg cggcgtgctc tactgccgca ttgtgccgcc gatgaccggg aaagcgattc
    24421 atgtgatgtg cgtacatctg ggcctgcgtg aggcgcaccg tcaggcgcag cttgcgatgc
    24481 tcgccgaatg ggtgaatgag ctgccggacg gcgaaccagt attggtggcg ggtgatttca
    24541 acgactggcg gcaaaaagct aatcatccgt taaaaatgca ggccgggctg gatgagatat
    24601 ttacccgcgc ccacggacgc ccggcgcgca cgtttccggt gcaatttcct ctactacgtc
    24661 tggacaggat ctatgtcaaa aatgccagcg ccagcgcgcc aaccgcgttg ccgctgcgga
    24721 catggcgaca cctttctgat catgcccctt taagtgcgga gattcattta tgaaatgtag
    24781 ctggcgcgaa ggcaataaga tccagttgct ggaaaacggc gagcaatatt atcccgcgat
    24841 gtttaaggcg attggcgagg cgcaagaacg catcattctt gaaacgttta tctggtttga
    24901 ggatgacgtc ggcaaacagc tgcatgcggc gctactggca gcagcgcaac gcggggtgaa
    24961 agcggaagtc ttgctggatg gctacggttc gccggatctc agcgatgagt ttgtcaatga
    25021 actgacggca gctggcgtgg tatttcgcta ctacgatccc cgtcctcgcc tttttggtat
    25081 gcgcaccaat gtgtttcgcc ggatgcatcg caaaattgtg gtgatcgacg cgcgtatcgc
    25141 ctttattggc gggctgaatt actccgccga gcatatgtcc agctacggtc cagaggctaa
    25201 acaggattac gcggtacgcc ttgaagggcc gattgtcgaa gatattctcc agtttgagct
    25261 ggaaaacctg cctggacaga gcgcggcacg acgctggtgg cgacgtcatc acaaagcgga
    25321 agagaaccgc cagccgggag aagcgcaggt attgctggtc tggcgcgata acgaagaaca
    25381 tcgcgatgat attgaacgtc actatctgaa aatgctcact caggcgcggc gagaagtcat
    25441 tatcgccaac gcctacttct tccccggcta tcgattttta cacgccttgc gtaaagcggc
    25501 acggcgcggg gtgcggatca aactgatcat tcagggcgaa ccggatatgc cgattgtcag
    25561 agtcggtgcg cgcttgctgt ataactatct ggttaaaggc ggcgttcagg tatttgagta
    25621 ccgccgccgc ccgctccacg gcaaagtggc attgatggac gatcactggg cgacagtagg
    25681 gtccagtaat ctcgatccgc tcagtttgtc actgaatctc gaagcaaatg tcatcatcca
    25741 cgatcgtcat tttaaccaga cgctgcgcga taatctgaac ggcattattg ccgcagattg
    25801 tcagcaggtc gatgagacca tgctgccgaa acgcacctgg tggaacctga ccaaaagcgt
    25861 gctggcgttc cactttttac gccacttccc ggcgctggtt ggctggcttc cggcacatac
    25921 accacgtctg gcgcaggttg atccccccgc acaaccgaca atggaaacgc aggatcgggt
    25981 agaaactgaa aacacggggg taaaaccctg atgagtaaat cacacccgcg ctggcgctta
    26041 gcaaagaaga tcctcacctg gctgtttttt atcgcggtga ttgtgttgct ggtggtctac
    26101 gccaaaaaag tggactggga agaggtctgg aaggtcatcc gcgactacaa tcgcgttgcg
    26161 ctgctcagtg cggtcgggct ggtggtcgtc agctatctga tttacggctg ctatgacctg
    26221 ctcgcccgtt tctactgcgg tcacaaactg gcgaagcgcc aggtgatgct ggtgtcgttt
    26281 atctgctacg ccttcaacct gacgctcagt acctgggtcg gcggcatagg tatgcgctat
    26341 cgtttgtact cgcggctagg gttaccgggc agcactatta cgcggatttt ctcgctcagt
    26401 attaccacca actggctggg ctatattttg ctggccggga ttatctttac cgcaggcgtg
    26461 gtggagctgc cggatcactg gtatgtcgat caaaccacgc tgcgtattct cggcattggc
    26521 ttattgatga ttatcgcggt ttatttgtgg ttttgcgctt tcgcgaagca ccgccatatg
    26581 accatcaaag ggcaaaagct ggtgctgcct tcatggaaat tcgccctcgc ccagatgctg
    26641 atttccagcg ttaactggat ggtcatggga gcgataatct ggctgttact tggtcaaagc
    26701 gtgaactatt tctttgtact gggcgtgttg ctggttagta gtattgccgg ggtgattgtg
    26761 catattccgg cggggattgg tgtgctggaa gcggtgttta tcgcactact ggctggggag
    26821 catacctcca aaggtagcat tatcgccgcc ctactcgctt accgtgtgct gtattacttt
    26881 atcccactgc tgctggcgct gatctgttac ctgctactgg aaagccaggc gaagaagctg
    26941 cgggcgaaaa atgaagcggc gatgtgaagt tcggttaagt tctttagtat gtgcatttac
    27001 gactaatgaa aaaaccgcgt atgcctttgc cagacaagcg ttatagctat cgcggcaagt
    27061 atcccaaaga agccgataaa atagtaatag agtgttaatg cacctgtaaa agcgcaacta
    27121 accggactcc acaactcttc gctatggcat tttcctgcca gttcaaggag cttatttcgt
    27181 ccatgcgaga taatgcctga ccataaaaca atcattaagc aacttattac ccaaatgggc
    27241 cgttcactca ttaatgcggc attcctgatg atcaccaggg ccagtcctgc ggttgtcacc
    27301 aaacccaaag cggtaatgag ccatatgccg tttaatatcc tggcttcgtg ctgaaaatct
    27361 aatttattgc tgctttgcga gtatgattcc attcttttag ttatccttag aaaaaatatt
    27421 aatattcacc atcaataaat ttaaagccac gcatacaaaa tacttttgta tttatggata
    27481 cggaaaacac ttccgctcgg ccataaggca tattttatac ttggtacttg ttaagtgtcg
    27541 ttttttcgta agaaaaaaat cccgacacat ggccgggata ttgatggtga aaaagaatta
    27601 acggcggttg ccgaagatcc gcaacaacat caggaacaag ttgatgaagt ccagatacaa
    27661 ggttaacgcg ccaagaatgg aatatttgcg caggttcgac gtgtcgcggg tatcaatctg
    27721 ctcacccata tttttcaatt tctgcgtgtc atacgccgtc aatccgacaa agacaatcac
    27781 gccaatgtag gtaactgccc acatcaatgc ttcgcttttc agccagaagt tgaccagcga
    27841 tgccagcaca atgccgatta acgccataaa cagcatattg ccaaagccgc ttagatcgcg
    27901 cttcgtggtg taaccgtaca ggctcattgc gccaaacatt ccggcagtaa cgacgaaagt
    27961 actggcgata gaagcagcgg tataaacaat gaaaatactg gaaagcgtaa gacccgtcag
    28021 cgccgaataa agcataaaga gcatcgtcgt tacacctgcg ctcagctttt gaatcatcgc
    28081 tgataacaca ataaccaatg ctaattgcgc gatgatcaga ccgattaaaa agacacggtt
    28141 agtgaacaac agctccatca cagccgcgga attagccgca taccaggcaa caaatgcggt
    28201 cagcaacaag ccaacggtca tccagccata gacttgcgcc atataagttt gcaagccagc
    28261 ccggggttgt acaattgaat cagaacgtgg gaatctgtcc atgacgatct cctgaagata
    28321 taaggaatat cttaaggata ctgcaaaatg atgaggctgt gcatcgacgc agcgtaaacg
    28381 catgtactga gcggtgaaat tgccggacgc agcggtgcct tatccggcta acaaaaaact
    28441 accagcgttt tgccgcctgc tgatcgctct cccgagcttc aacccagcgg tcgccttccg
    28501 gcgtggcttc acgcttccag aacggtgcgc gggttttgag ataatccata ataaactgcc
    28561 cggcttcaaa cgcactgctg cgatgcgcac tggtgacacc gacaaaaacg atttcatctc
    28621 ccggccataa ttccccgatg cggtgaatca ccgtgacgcg ccccagcggc cagcggttac
    28681 gcgcttcatc gacaatttct gccagtgctt tttcagtcat ccccggatag tgttcgaggg
    28741 ttaatgcttt gacgctgtcg ccaaggttat ggttgcgcac cttaccggta aaggtgacta
    28801 ccgcaccgtc ttcatcacgc tccgccagcc acggatactc ttctcctacg ctgaatggct
    28861 gcgggccgac aacaattttg gtttctgcca tcttaacctc cggttaccgg cgggaagaaa
    28921 gccacttcgt cgccgtcagt cagcggatgg tcaagactca ccagcgtctg gttgacggca
    28981 gccagcaatt tgccatcttc cagcgccagc gcccagcgat cgctctgcgc agccagatgt
    29041 tggcgtaacg cttcaacggt tgggaaatcc gcagccactt cggttgcatc tgttcccacc
    29101 aactcgcgca cctgggcgaa aaaaagaact ttaatcatcc gcttccacct taaagtcacc
    29161 cgacttgccg ccgctcttcg ccagcaaacg taccggacca attaccatat ctttttgcac
    29221 cgctttgcac atgtcataaa tggtcagcgc cgccacggag gccgcggtta acgcttccat
    29281 ttcgacaccg gttttcccgg tcaggcggca taaggtttct atacgtaccc gattgtgctc
    29341 cggctcggcc tgtaaattga cttcaacttt gctgagcatc agcgggtgac acagcgggat
    29401 cagatcccag gtgcgttttg ccgcctgaat accggcgata cgcgcagtgg caaatacgtc
    29461 gcctttgtgg tggcgaccat caataatcat cgccagcgtc tcgctgcgca tggtgacaaa
    29521 ggcttccgcc cgcgcttcac gcacggtttc cgctttggcg gagacatcca ccatgtgtgc
    29581 ttcgccagcg gcgttgatat gggtcagttg cgacatactt atttcttcaa atgtggatgg
    29641 aaattgcatg gacgtgtacg ggcatcgagc tgcggcgcga tgatattttc ccacgcggtg
    29701 cggcacgctt tggtcgaacc cggcatggcg aaaatcagcg ttttgttagc gacgcccgct
    29761 accgcacgag attgcaacgt tgaggtgcca atctcttcaa acgacaacat ccggaacact
    29821 tcaccaaaac cttcaacttc acggtcgaac aacggcagca gcgcttcggg agcctgatca
    29881 ccttccgtca ggccagtacc acccgtaatc aataccactt gtacatcgtc gctggcgatc
    29941 cacgcagata cctgagcgcg aatagcgtag cggttttctt tcacaatggc tttatcgaca
    30001 acgtgatggc ccgcttcctg cgccgaatcg cgcagatagt gaccggaggt atcgtcttct
    30061 tcaccacgac gattagaaac cgtaagaata gcaatacggg tcgggataaa ttcagtgctt
    30121 acctgactca tctgatctct ccttttgacg ttttagccgc caatgtacga taagttttgc
    30181 gtaataccgg tgttgttttg atgcaggaaa tgggtctgtt tcttctcccg cagcgccgct
    30241 gaaatacgcg cttccagcgc ctgttgctgg gtatcgtctt ccagcagatc gcgcaggtta
    30301 acgccgcctt caccaaacag gcagagatgg agtttaccaa tggaggaaac gcgcaggcgg
    30361 ttgcaagtgg cgcagaagtc tttttcatac ggcatgataa ggccaatctc tccggcgtaa
    30421 tccggatgac aaaagacttg cgcgggaccg tcgctgcgtt gacgtaattg gtggatccag
    30481 ccgcgacgca gtagctcgtc acgcagaacc tgaccagaga tgtgatgctt acggaagagc
    30541 tcgctgccct cacccgtttc catcaattcg atgaaacgca gctggatagg gcgatgctgg
    30601 atccagttca gaaaggtgtc gagctgatga tgattaacat cacgcatcag cacggtattg
    30661 actttgacct tctcaaaacc ggcctcaaat gcagcatcga tccctgccat gacctggttg
    30721 aatttatcct gcccggtaat ggcatgaaac tggcgagcgt caaggctatc aacgctgacg
    30781 ttgatgccag taagtcccgc atcgcgccag ttcgccacat cgcgttccag acggtaacca
    30841 ttggtggtga ccgcaatctg gcggatagcg tcgttttccc gcacagcggc gatgatatcg
    30901 gtaaagtcgc ggcgtaaaga cggctcaccg cctgtcaggc gcactttttc ggtgcccaga
    30961 ctggcaaagg cgcgcgtaac ccggcgaatt tcatcgacgg taagaaagcc tttattggtg
    31021 acgccgctcg gtttgtagcc atccggcagg cagtaggtgc aacgaaagtt acacacatca
    31081 gtaatcgaca ggcgcaagta gtaaaactta cgcgcaaatg catcagtcag ttgtgaagcc
    31141 atgtacacct ttccagatac gggaggcgaa gtcatttctt ccttcgccct ggtggcaatc
    31201 tttacgagca ttgtcacggc caaagcaccg tatcagttga cccaggtgca gaggctagag
    31261 tgtttagtgg ttaagccgat actagcgtgt aaatgagatt tttaccatcc acactttcgc
    31321 tatgtaatca tgtatatagc gtcatgatcg tgctatttcg ctacagaata agggaaaatg
    31381 gcggttttac tttgatatac atcatcagga aggcatagcg tacatcgtcc tttagaggta
    31441 gaagaaaata gtttgtatcc tcaatattgg caggttaatt gctgtttccc cgcaatttgc
    31501 gctactgtag cgcggataat ctgactccag gaaactatat gcgcaatcgt acgctggctg
    31561 accttgatcg tgtcgttgct ctcggcggag ggcatggact gggacgcgtt ctctcatcac
    31621 tttcgtcttt gggttctcgt ttaacgggta tcgtcaccac cacggataat ggcggatcga
    31681 cagggcgtat tcgccgctca gaaggcggta ttgcctgggg cgatatgcgt aactgcctca
    31741 accagctgat aactgaaccc agcgtcgctt ccgcaatgtt tgaatatcgt ttcggcggta
    31801 atggcgaact ttccgggcac aaccttggaa atctgatgct gaaggcactg gatcacctta
    31861 gcgtgcgacc tcttgaagca atcaatctga ttcgcaatct gctgaaagtg gacgcacatt
    31921 tgattccgat gtcggagcat ccggtggatc tgatggcgat tgacgatcag gggcatgaag
    31981 tttacggcga ggtcaatatc gaccagttaa ctacgccgat tcaggagtta ctgttaacgc
    32041 cgaatgtacc cgcaacgcgt gaggctgttc acgctatcag tgaagcggat ctcatcatta
    32101 ttgggcctgg cagtttttac accagcctga tgccaattct gctgctgaag gaaatcgcac
    32161 aggcattacg ccgcacgcca gcgccgatgg tctatatagg caatctgggg cgtgagttga
    32221 gtttacctgc ggctaatttg aagctggaaa gcaagctggc aattatggag cagtatgtcg
    32281 gtaaaaaggt cattgatgcg gtcatcgtcg ggccaaaagt ggatgtctcg gcggtgaaag
    32341 agcggattgt gattcaggag gtactggagg ccagcgatat tccgtatcgt catgaccgcc
    32401 agttgttaca tagcgcgctg gaaaaggcat tacaggcttt aggttaacgg aaagccgaag
    32461 gaaaattccg gcttcctatt gaagacaaag tgcgcgttgt ttatgccgga tgcggcgtga
    32521 aagccttatc cggcctacaa accgcgctaa ttcaatgtat tgcagagatc atgtaggcct
    32581 gataagcgta gcacatcagg caattttgct tttgtcatct gtcttcttcg ttatcctgtc
    32641 acgaagccgc aatgaacaac tcacgcagct gatgcaactg gtcacgaatt tgtgccgctt
    32701 cttcgaactc cagattctgc gcgtgttgca tcatcaaccc ttccagttca tggattttct
    32761 gctgcaacgc tttaggcgac atatccatcg gcacattatc cggctcaaca atcggtcgcg
    32821 attttcctct gcccttcgct ttggttttgg caatgttctg ccccagcgcc aggatatcga
    32881 ccactttctt gttcaagcct tgcggcgtaa tgccgtgttc ctcgttgtac ttctgctgtt
    32941 tctcgcggcg acgttcggtt tcgccaatcg ctttcgccat tgatggggtg atcttatcgc
    33001 cgtagagaat cgctttaccg ttaacgttac gtgccgcacg accaatggtc tggatcaacg
    33061 aacgttcgga acgcaggaag ccttctttgt cagcgtcgag gatcgccacc agcgaaactt
    33121 ccggcatatc cagaccttcg cgcagtaagt tgatccctac caatacgtcg aactcaccca
    33181 gacgcaagtc gcggataatc tccatacgtt cgacggtgtc gatatctgag tgaagataac
    33241 gcacgcgctc accgtgttct tcgagatatt cagtgagatc ttccgccatc cgcttggtca
    33301 gagttgtaac cagtacgcgt tcgttaattg ccgctcgctg acgaatctcc gaaagaagat
    33361 catccacctg tgttgccact ggccgcactt cgataatcgg gtcgagtaag cctgttggac
    33421 gcaccacctg atccaccaca tcgccaccgg atttttccag ctcgtaatta cccggcgtcg
    33481 ccgaaacata gatggtttgc ggcgctaatg cttcgaactc ttcaaatttc agcggacggt
    33541 tatccagcgc tgatggcagg cggaagccgt actccaccag tgtctcttta cgcgcccggt
    33601 caccgcgata catgccgcca atttgtggaa tggtgacgtg agattcatcg accaccagca
    33661 gcccatcagc aggcaggtaa tcaaacagcg tcggcggtgg ctcacccggt ccacgaccgg
    33721 agaggaagcg cgagtagttc tcaatccccg aacagtagcc tagctcgttc atcatctcca
    33781 gatcaaactg ggtacgctgg gtcagccgct gctcttccag cagtttgttg ttttccaata
    33841 gcactttacg tctggcggcc agctcttctt tgatctcctc catcgcctgg acgatgcgct
    33901 cgcgtggcgt gacgtagtgc gttttcgggt agatggtaaa acgtggaata gtggaaacaa
    33961 tctgcccggt cagcgggtca aataacgaca atcgttccac ttcctcgtca aacagctcca
    34021 cgcgaagtgc aatgtcatcc gattctgccg ggaagatatc aatcacctcg ccacgaacac
    34081 ggaaagtacc acgctggaag gcttgatcat tacgagcata ttgcagctcc gccagtcggc
    34141 gcaggatcgc gcgctgatcg ataatcatac cgaccgtgag atggagcatc atcttgagat
    34201 ataaatcagg atcgcccaga ccataaatcg cggaaacaga cgccaccaca accacatcac
    34261 gccgctccag catcgctttg gtggcggaca aacgcatctg ttcgatatgt tcgtttaccg
    34321 aggcatcttt ctcaatgaag gtgtcggaac tcggtacata ggcctccggc tgatagtagt
    34381 cgtagtagga gacgaaatat tccaccgcgt tttccgggaa gaattctttc atttcgccat
    34441 acagctgggc cgccagcgtt ttgttgggcg caagtaccat ggttgggcgc tgaaggtcag
    34501 caatgacatt ggcaatggtg aaggttttcc cggagccagt cacgccaagt aacgtctggt
    34561 gcgccaggcc atcttccagc ccctcttcga gacgtcgaat cgcctccggc tgatcgccag
    34621 aaggtttaaa agcggaattc agtttgaacg gtttactcat gagtcgctac ctgaaggagt
    34681 tgggcgggca ggtatgtaat tttactcgtc gtacttaatt atgccaacaa attatactgg
    34741 ataaaaaaac agttcatcac cataatattt ctgttacagc gtaaactccg ctgtcaatca
    34801 tgagcaaaat ttactctgcg gcgagataaa actccggcct taccgggtta tccccaaagc
    34861 aacggctttt ttaacatttg tcaagatgat tgatgacagt tttatggcaa gagatgcctg
    34921 ttcagtgact gccattgcaa ttatctaacc tgttaaaaaa taaaagatat ttctttgagc
    34981 cgtctttaac gccaattcgc gtgcaagccg cgtattctct cgcttgcctc gtgttttcta
    35041 actctaatac acatggttat ccacaggaat agtggataac tgtctgcagc ccctatcccc
    35101 cgccgcctgg g
//
LOCUS       DAZUCC010000022        34867 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZUCC010000022 DAZUCC010000000
VERSION     DAZUCC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.022"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            174..326
                     /gene="mokC"
                     /locus_tag="ACHY09_000979"
     CDS             174..326
                     /gene="mokC"
                     /locus_tag="ACHY09_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFS3883982.1"
                     /translation="MKQPKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(430..1560)
                     /gene="dnaJ"
                     /locus_tag="ACHY09_000980"
     CDS             complement(430..1560)
                     /gene="dnaJ"
                     /locus_tag="ACHY09_000980"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS3883983.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1649..3565)
                     /gene="dnaK"
                     /locus_tag="ACHY09_000981"
     CDS             complement(1649..3565)
                     /gene="dnaK"
                     /locus_tag="ACHY09_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS3883984.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEVGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3941..4345
                     /gene="yaaI"
                     /locus_tag="ACHY09_000982"
     CDS             3941..4345
                     /gene="yaaI"
                     /locus_tag="ACHY09_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFS3883985.1"
                     /translation="MKSVFTLSASLAISLLLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4371..5084
                     /gene="msyB"
                     /locus_tag="ACHY09_000983"
     CDS             4371..5084
                     /gene="msyB"
                     /locus_tag="ACHY09_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFS3883986.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5233..5799
                     /gene="satP"
                     /locus_tag="ACHY09_000984"
     CDS             5233..5799
                     /gene="satP"
                     /locus_tag="ACHY09_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFS3883987.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5834..6421)
                     /gene="mog"
                     /locus_tag="ACHY09_000985"
     CDS             complement(5834..6421)
                     /gene="mog"
                     /locus_tag="ACHY09_000985"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFS3883988.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYIETAPEVVAAFRPKSARRDVSE"
     gene            complement(6536..7489)
                     /gene="tal"
                     /locus_tag="ACHY09_000986"
     CDS             complement(6536..7489)
                     /gene="tal"
                     /locus_tag="ACHY09_000986"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFS3883989.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            7768..9198
                     /gene="yaaJ"
                     /locus_tag="ACHY09_000987"
     CDS             7768..9198
                     /gene="yaaJ"
                     /locus_tag="ACHY09_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFS3883990.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9268..10044
                     /gene="yaaA"
                     /locus_tag="ACHY09_000988"
     CDS             9268..10044
                     /gene="yaaA"
                     /locus_tag="ACHY09_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFS3883991.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10084..10380)
                     /locus_tag="ACHY09_000989"
     CDS             complement(10084..10380)
                     /locus_tag="ACHY09_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFS3883992.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEMTLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(10594..11880)
                     /gene="thrC"
                     /locus_tag="ACHY09_000990"
     CDS             complement(10594..11880)
                     /gene="thrC"
                     /locus_tag="ACHY09_000990"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFS3883993.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFVFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLHPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11881..12813)
                     /gene="thrB"
                     /locus_tag="ACHY09_000991"
     CDS             complement(11881..12813)
                     /gene="thrB"
                     /locus_tag="ACHY09_000991"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFS3883994.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12815..15277)
                     /gene="thrA"
                     /locus_tag="ACHY09_000992"
     CDS             complement(12815..15277)
                     /gene="thrA"
                     /locus_tag="ACHY09_000992"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFS3883995.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15303..15422)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15358..15423)
                     /gene="thrL"
                     /locus_tag="ACHY09_000993"
     CDS             complement(15358..15423)
                     /gene="thrL"
                     /locus_tag="ACHY09_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFS3883996.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15637..16323)
                     /gene="yjtD"
                     /locus_tag="ACHY09_000994"
     CDS             complement(15637..16323)
                     /gene="yjtD"
                     /locus_tag="ACHY09_000994"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFS3883997.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADKHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16723..16863)
                     /gene="yjjY"
                     /locus_tag="ACHY09_000995"
     CDS             complement(16723..16863)
                     /gene="yjjY"
                     /locus_tag="ACHY09_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFS3883998.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            16959..17675
                     /gene="arcA"
                     /locus_tag="ACHY09_000996"
     CDS             16959..17675
                     /gene="arcA"
                     /locus_tag="ACHY09_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFS3883999.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17734..19086)
                     /gene="creD"
                     /locus_tag="ACHY09_000997"
     CDS             complement(17734..19086)
                     /gene="creD"
                     /locus_tag="ACHY09_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFS3884000.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAHWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFIAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGAIMNGIYLQAVLKGWCNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19144..20568)
                     /gene="creC"
                     /locus_tag="ACHY09_000998"
     CDS             complement(19144..20568)
                     /gene="creC"
                     /locus_tag="ACHY09_000998"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFS3884001.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDTTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPTDPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20568..21257)
                     /gene="creB"
                     /locus_tag="ACHY09_000999"
     CDS             complement(20568..21257)
                     /gene="creB"
                     /locus_tag="ACHY09_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFS3884002.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLTLHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21270..21743)
                     /gene="creA"
                     /locus_tag="ACHY09_001000"
     CDS             complement(21270..21743)
                     /gene="creA"
                     /locus_tag="ACHY09_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFS3884003.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21954..22823
                     /gene="robA"
                     /locus_tag="ACHY09_001001"
     CDS             21954..22823
                     /gene="robA"
                     /locus_tag="ACHY09_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFS3884004.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22820..23467)
                     /gene="gpmB"
                     /locus_tag="ACHY09_001002"
     CDS             complement(22820..23467)
                     /gene="gpmB"
                     /locus_tag="ACHY09_001002"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFS3884005.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23519..24034
                     /gene="yjjX"
                     /locus_tag="ACHY09_001003"
     CDS             23519..24034
                     /gene="yjjX"
                     /locus_tag="ACHY09_001003"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFS3884006.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            complement(24028..24354)
                     /gene="trpR"
                     /locus_tag="ACHY09_001004"
     CDS             complement(24028..24354)
                     /gene="trpR"
                     /locus_tag="ACHY09_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFS3884007.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(24444..26381)
                     /gene="sltY"
                     /locus_tag="ACHY09_001005"
     CDS             complement(24444..26381)
                     /gene="sltY"
                     /locus_tag="ACHY09_001005"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFS3884008.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWELSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26717..28384
                     /gene="ettA"
                     /locus_tag="ACHY09_001006"
     CDS             26717..28384
                     /gene="ettA"
                     /locus_tag="ACHY09_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFS3884009.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28440..28724)
                     /gene="nadS"
                     /locus_tag="ACHY09_001007"
     CDS             complement(28440..28724)
                     /gene="nadS"
                     /locus_tag="ACHY09_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFS3884010.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPDFFRELASH"
     gene            complement(28726..29058)
                     /locus_tag="ACHY09_001008"
     CDS             complement(28726..29058)
                     /locus_tag="ACHY09_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3884011.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(29150..30382)
                     /gene="nadR"
                     /locus_tag="ACHY09_001009"
     CDS             complement(29150..30382)
                     /gene="nadR"
                     /locus_tag="ACHY09_001009"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFS3884012.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30403..31785)
                     /gene="radA"
                     /locus_tag="ACHY09_001010"
     CDS             complement(30403..31785)
                     /gene="radA"
                     /locus_tag="ACHY09_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS3884013.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31834..32802)
                     /gene="serB"
                     /locus_tag="ACHY09_001011"
     CDS             complement(31834..32802)
                     /gene="serB"
                     /locus_tag="ACHY09_001011"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFS3884014.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            32908..33552
                     /gene="ytjB"
                     /locus_tag="ACHY09_001012"
     CDS             32908..33552
                     /gene="ytjB"
                     /locus_tag="ACHY09_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFS3884015.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33580..34596
                     /gene="lplA"
                     /locus_tag="ACHY09_001013"
     CDS             33580..34596
                     /gene="lplA"
                     /locus_tag="ACHY09_001013"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFS3884016.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            34628..>34867
                     /locus_tag="ACHY09_001014"
     CDS             34628..>34867
                     /locus_tag="ACHY09_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3884017.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPS"
BASE COUNT         8203 a         9476 c         8671 g         8517 t
ORIGIN      
        1 aaagagagcg atataatagc ggcgggtgct tgaggctgtc tgtctcaggc attagctgaa
       61 cggcagatag agaaaagccc cgagtgatat tttactatca acccgaggcc ccctatatgc
      121 agaacacatg tagagtgcct cttactgacc gtaaggtcaa ggagaagaga gcaatgaagc
      181 agcctaaggc gatgattgtc gccctgatcg tcatctgtat caccgccgta gtggcggcgc
      241 tggtaacgag aaaagacctc tgtgaggttc acatccgaac tggccagacg gaggttgctg
      301 ttttcacggc ttacgaatcc gagtaagagc aacggcgggg agtgatcccc gccacttttc
      361 aggtgctcgc atatcttcaa cgcaccctat ttttacccag gcctgcccac gggcaggctt
      421 ttggggagat tagcgggtca ggtcgtcaaa aaacttcttc acaccatcaa agaagctctt
      481 tgagcgcggg ctgttgtgct cgccggttgg gccaccgaag ctttcttgca gctcttgcag
      541 cagctgtttc tgcttctcgt tcaggcctac cggtgtttcg acgacaacgc ggcacagcaa
      601 atcaccctgt gcgccaccgc ggacagactt gacgccttta ccgcgcatac ggaacagctt
      661 accggtctgg gtttcgccag gcactttcag tttgacgcga ccatcaaggg tcggtacttc
      721 gatttcgcca cccagcgccg ccatagcgaa gttgatcggg acttcgcaat agaggttgtt
      781 gccttcacgc tcgaaaatcg ggtgctgttt aacctgaacc tgaacgtaca gatcgcctgc
      841 cggtgcgcca tgctcgcccg cttcaccttc gcccgcaaga cggatgcggt ctccagtgtc
      901 cacccctgcc gggattttaa cggacagcgt tttgctgcgc tcaacacgac catgaccatg
      961 acatttgttg cacggatctt tgatcagcgt accgcggccc tgacagtgtg gacaggtctg
     1021 ctgcacggca aagaaaccct ggcgcatctg cacctgacca gaaccatgac aggtcggaca
     1081 ggtctgcggc tgtgtacctg gttttgcacc gctaccgtgg caaacgtcac actcttccag
     1141 agtcggaatg cggatctctt tggtcacgcc acgtacagct tcttcgaggg tgagctccat
     1201 gttatagcgt aaatcagcac cgcgcgccgc acgttgacga ccacgtccgc cgccaaaaat
     1261 atcgccgaaa acgtcaccaa aaatatcgct gaagtctgcg ccgccgccga aaccgccgcc
     1321 tcccatgcca ccttgctcaa acgcagcatg accatactga tcgtatgccg cacgtttttg
     1381 cgagtcggtc agaacttcat aagcttcctt gatctcttta aatttcgcct cggcctcttt
     1441 gtcaccctgg ttacggtccg ggtggtattt catggccagg cgtttgtagg cctttttgat
     1501 ttcacgctct tccgctgttt tggaaacgcc taaaatctcg taataatctt gcttagccat
     1561 cttttttaaa ttgcccctag atgaatgcac gggcggagag gaaattcccc ttcgcccgtg
     1621 tcagtataat tacccgttta tagggcgatt attttttgtc tttgacttct tcaaattcag
     1681 cgtcgacaac atcgtcatct ttcgcgttgt ttgcagaagc atcagcaccg gcagtctgct
     1741 gctgggcatg ttgctgctgg gcgatttcca tcagtttctg ggaaacctgt gccagttcct
     1801 gcattttcgc ttcgatagcg gctttgtctt cacctttcag agcagtttcc agtgcagtca
     1861 gtgcagactc gatagcagtt ttgtcgtcag ccggcagttt gtcgcccact tcttcaacct
     1921 gcttacgggt gctgtgcagc agatggtcgc cctggttgcg agtctgtacc agctcttcaa
     1981 acttacggtc agcttcggcg ttagcttctg cgtcgcgtac cattttctgg atttcatctt
     2041 cgttcagacc agaagaagcc ttgatggtga tcttctgctc tttaccgctg tttttatctt
     2101 tcgcggaaac gtgcaggata ccgtcagcat cgatatcgaa ggtaacttcg atctgcggca
     2161 tgccgcgcgg tgccgggttg ataccatcca ggttgaactg acccagagat ttgttatcag
     2221 ctgcacgttt acgttcaccc tgcagcacat ggatggttac cgcagactgg ttgtcttcag
     2281 cggtagagaa cacctggctg tgcttggtcg ggatagtggt gtttttcgcg atcagcgtcg
     2341 tcatcacacc gcccatggtt tcgataccca gagacagcgg ggtaacgtcc agcagcagta
     2401 cgtcttttac gtcaccagtc agaacaccac cctgaacagc agcaccgatt gctacagctt
     2461 cgtccgggtt aacgtcttta cgcggctctt taccaaagaa ctcagcaact ttcttctgaa
     2521 ccattggcat acgagtctga ccaccaacga ggataacgtc gtcgatatca gatacggaca
     2581 ggccagcgtc ctgcagtgca actttcagcg gctcaatgga acggtttacc agatcttcaa
     2641 ccaggctttc cagtttcgca cgagtcactt tgatgttcat gtgtttcgga ccggtcgcgt
     2701 ctgcagtgat gtacggcagg ttaacgtcgg tctgctgagc ggaagacagt tcgattttcg
     2761 ctttttctgc cgcttctttc aggcgctgca ttgccagcgg atcgttgcgc aggtcaatgc
     2821 cctgatcttt cttgaattct tcaaccaggt agttgatcag acggctgtcg aagtcttcac
     2881 cacccaggtg ggtatcaccg ttggttgcca gaacttcgaa ggttttttcg ccgtcaactt
     2941 cgtcgatttc gataatagaa atatcgaaag taccaccacc caggtcataa accgcgatag
     3001 tacggttgcc agtacctttg tccagaccgt aagccagcgc agctgcggtc ggttcgttga
     3061 tgatacgttt tacttccaga ccagcgatac ggcctgcgtc tttggttgcc tgacgctgag
     3121 catcgttaaa gtatgccggt acggtgataa cagcttcagt taccggttca cccaggtaat
     3181 cttcagcggt tttcttcatt tttttcagca cttcagcaga gatctgcggc ggtgccattt
     3241 tctggccttt aacttcgacc catgcgtcgc cgttatcagc agcaataatt ttgaacggca
     3301 tgatggaaac atcacgctgt acttcttcgt cctggaagcg gcggccaatc aggcgtttaa
     3361 tcgcaaacag ggtgttttgc gggttcgtca ctgcctgacg tttagccggc tgaccaacca
     3421 gagtttcacc atcctgggta taggcaatga tagaaggcgt ggtgcgatcg ccttcggcgt
     3481 tctccagtac acgaggagtg gtgccatcca taatcgctac acaagagttg gtagtaccca
     3541 ggtcgatacc aattatttta cccatctaaa cgtctccact atatattcgg tcatcatgtg
     3601 gttgtgagtc tgtaataggg gcgaaacgtc tggtttcaac tgcccaattt catttttttg
     3661 cagactcact cactgcggtt gactactaaa tggggtcgta acccacgtca tcaaggggga
     3721 gatgcaaaaa ttttttgtgc ttttgcgcag aaatttcgcg atatgccaga acaagacatg
     3781 aaactgacag caattttatt catatagaga atgaatagca gacaagacat tcatagaaaa
     3841 attgtaataa aaagaaaata ttacgtacct gaatcttctc tttatcagca gtaaactagt
     3901 gggtattcat cccctacctc tccccactaa gagaatcctt atgaaatccg tttttacgct
     3961 ttccgccagc ctggcgatta gcctgctgct gtgctgcacg gcgcaggcaa acgaccataa
     4021 aatcctcggc gtcattgcaa tgccacgtaa cgaaaccaac gatctggcgc tgaaacttcc
     4081 tgtttgccga attgttaaac gcatacaact ctcagccgac catggcgatt tacagttaag
     4141 cggtgcatcg gtttatttca aagccgcccg tagcgccagt cagaccctga atattccttc
     4201 agaaataaaa gaagggcaaa ccactgactg gatcaacatt aacagcgata acgacaataa
     4261 acgctgcgtc tcaaaaatca ccttttctgg tcatacggtg aactcatcgg atatggccac
     4321 gctgaaaatt atcggcgacg actaacgcca gaatatagaa gccacaaaaa atgaatgtta
     4381 attacctgaa tgattcagat ctggattttc tccagcattg tagtgaggaa caactggcaa
     4441 atttcgcccg gctgctcacc cataatgaaa aaggcaaaac tcgcctctcc agcgtactga
     4501 tgcgcaacga actgtttaaa tcgatggaag gacatcccga gcaacaccgc cgcaactggc
     4561 agctgattgc cggagaatta caacattttg gtggcgatag tatcgccaac aaactgcgcg
     4621 gacacggtaa attgtatcgg gccattttgc tcgatgtttc aaaacgattg aagctgaaag
     4681 ccgacaaaga gatgtctacg tttgaaattg agcaacagtt actggaacaa tttctgcgta
     4741 atacctggaa gaaaatggac gaggaacata agcaggagtt tctgcacgcg gtcgatgcca
     4801 gggtgaatga gctggaagag ctgctgccgc tgctgatgaa agacaaatta ttggcaaaag
     4861 gcgtgtcgca tttgctttcc agccaattga cccgcatttt acgcacccac gcagcaatga
     4921 gcgtacttgg gcatggtttg cttcgcggcg cggggctggg aggccctgta ggcgcggcac
     4981 taaatggggt taaagcggtc agcggcagtg cctatcgcgt gacaattcca gccgtactgc
     5041 aaatcgcctg cctgcgccgg atggttagtg ccactcaggt ctgataccgg aaaccgggga
     5101 aaattttcat tttcccctca aaagatcgta gacactgccc cactggctga ttattatgcc
     5161 gcgccctgaa aacactacag ttattcaggg aaattatttc accattcatt cgatgatgat
     5221 ttttgaggaa ttatgggcaa cactaagttg gctaatccgg caccgctggg cctgatgggc
     5281 ttcggcatga ccaccattct gcttaacctg cacaacgtgg gttattttgc tctggacggt
     5341 attattcttg ccatgggcat tttctacggc ggcatcgcgc aaatttttgc tggtctgctg
     5401 gagtacaaaa aaggcaacac tttcggttta accgcattca cctcttacgg ttctttctgg
     5461 ctgacgctgg ttgcgattct gctgatgccg aaactgggtc tgaccgatgc gccaaatgca
     5521 cagttccttg gtgtctacct gggtctgtgg ggcgtattta cgctgtttat gttcttcggc
     5581 acgctgaaag gcgcacgcgt tctgcaattc gttttcttta gcctgaccgt gctgtttgcc
     5641 ctgctggcga tcggtaacat tgccggtaac gccgcaatca tccactttgc cggctggatt
     5701 ggtctgatct gcggtgccag cgcaatctat ctggcgatgg gtgaagtact gaacgagcag
     5761 tttggtcgca ccgttctgcc gattggtgaa tcccattaat tgttttgaga tccccccgct
     5821 cggggggatt tttttattcg ctaacgtcgc gtcttgcact cttcggtctg aatgctgcaa
     5881 ccacttccgg tgccgtttca atgtatggcc cttccagcaa ctgaatgcag tacggtacgc
     5941 tggcaaaaat accatgcacc acaacgttac ccgcagcgtc cttcacgcct tccagcgtct
     6001 ctttaatcga cttcggctga ccgggtaagt taaggatcag cgcctgtttg cgaatcacgc
     6061 ccacctgacg cgaaaggatc gcagttggta caaaatgcag gctgatctgg cgcatctgtt
     6121 caccaaagcc aggcatctcg cggtccgcta ctgccagcgt cgcatcgggc gttacgtcac
     6181 gacgcgccgg gccagttccg cccgtggtga gcaccagatg gcaactcatt tcatccacca
     6241 gctcacacaa cgtttgctcg atgatcgcct gctcatcggg gattaagcgg gtttccagtt
     6301 caaacggcgt ggttagcgcc gatgtcagcc attcttccag cgcagggatg cctttatcct
     6361 gataaacgcc gctggatgcg cgatcagaga tggaaactaa gccaatacgt aaagtattca
     6421 tcaagatttt tccgtttaaa cagtctcgtt aaacagaatg atacactgcg aagggagtga
     6481 cagacaggct tcagaaaagg tagcgtgacc gacttcccgg tcacgctaag aatgattaca
     6541 gcagatcgcc gatcattttt tccagttttt cctggtcaac agcaaactta cggatacctt
     6601 ccgccagttt atctactgcc attggatcct ggttatgctg ccacaggaac tcggactcag
     6661 tgatacgcgc cggacgcgct ttcacttcgc cggtgtaaga cagtttacgt tcgatagccc
     6721 cttcgctctc agccagctct ttcagcagcg ccggtgcgat ggtcagacgg tcgcagcctg
     6781 ccagttccag aatttcgccg atgttacgga agcttgcgcc catcaccacg gtttcataac
     6841 cgtgctcttt gtagtactgg tagatttcag atacagaaac cacgcccgga tcttctgccg
     6901 gagcgtactc tttcttgtcg gtattcgctt tgtaccagtc aagaatacgg ccaacaaacg
     6961 gcgagatcag gaacacgccc gcttccgcac aagcacgagc ctgagcgaag gagaacagca
     7021 gggtcaggtt acagttaata ccttcttttt ccagctgttc tgcagcacgg ataccctgcc
     7081 aggtagaagc cagtttgatc agaatacgat cgttgctaat ccctgcatcg ttgtagagtt
     7141 tgatcaggcg ttttgctttc gcaattgacg cttcggtgtc ataggaaaga cgcgcatcaa
     7201 cttcagttga gatacggccc ggaaccagtt tcaggatttc cagaccaata tttactgcca
     7261 gtttgtcggt cgcgtccacg atctgctgcg cgcgatcgtt gctctgctgt ttcgcccagg
     7321 cgacagcatc atcaatcaac ttacggtatt ccggaatctg cgctgcgtta agaatgagag
     7381 aagggttggt tgtggcatcc tgcggttgat acagcttcat tgccgcgatg tccccagtgt
     7441 cggccactac ggtggtgtac tgacgaaggg aggtcaattt gtccgtcatg atagtatttc
     7501 tctttaaaca gcttgttagg gggatgtaac cggtctgccc tgatgatatc acgacgcaac
     7561 gtcagcgcaa ccgccgacaa gacggttatc gccaggcgag actgtttcgg atttctgaca
     7621 ctgcgttgat atcgcccgcc atttttatac aaaacctcat gtatgctacg cagaagttat
     7681 caagtacctc gtagcgtata tacttcttaa acaattggta acgtttacac aggaaagtca
     7741 tcgcgaccgg caataagagg gatatgcatg ccagattttt tctcctttat taacagcgtc
     7801 ctttggggat cggtaatgat ttacctgctc ttcggcgcag gttgttggtt cacttttcgc
     7861 accggatttg tgcagtttcg ctacatccgc cagtttggca aaagtcttaa aaatagcatt
     7921 catccacagc caggcggttt aacctcattt cagtcattgt gtaccagtct tgcggcgcgc
     7981 gtgggtagcg gcaacctggc tggcgttgcg ctggctatta ccaccggtgg accaggtgcc
     8041 gtcttctgga tgtgggttgc cgcgtttatc ggcatggcga cctcgtttgc cgaatgttcc
     8101 cttgcacaac tttataaaga acgtgacgtt aatgggcagt ttcgtggcgg accggcatgg
     8161 tatatggcgc gcgggctggg gatgcgctgg atgggcgttc tgttcgccct ctttttgctc
     8221 atcgcctacg gcataatttt cagcggagtt caggcgaacg ccgttgcccg agccctgagt
     8281 ttttcttttg attttccccc gctggtgaca ggcattattc ttgctgtctt tgctctgctg
     8341 gcaatcactc gcggtcttca tggcgtcgcc cggctcatgc aggggtttgt cccgttgatg
     8401 gcgataatct gggtactgac cagcctggta atatgcgtaa tgaatatcgg gcaacttccc
     8461 cacgtcattt ggtctatttt tgagagtgct tttggctggc aggaagcggc aggcggcgcg
     8521 gcgggatata ccttaagcca ggcgattact aacggttttc agcgcagtat gttttccaat
     8581 gaggcgggaa tggggtcgac gccaaacgcg gcagcggcgg ctgcgtcctg gcctccgcat
     8641 cccgcagcgc aaggaattgt ccagatgatt ggcattttta tcgacaccct ggtcatctgt
     8701 acggcaagcg ccatgctgat attactggcg ggtaacggca caacctacat gccgctggaa
     8761 ggtattcagc ttatccagaa ggcgatgcgg gtactcatgg gttcctgggg tgctgagttt
     8821 gttaccctcg tggttattct gtttgccttc agctccatcg ttgccaacta catttatgcc
     8881 gaaaacaatc tcttcttctt acgcctgaac aaccctaaag cgatctggtg tctgcggatc
     8941 tgcaccttcg caacggtcat cggcggcacc ttgctaagtc ttccgctgat gtggcaactg
     9001 gcagatatca taatggcctg catggctatt accaatttga ccgccatatt actgctctcg
     9061 cctgtggttc ataccattgc cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg
     9121 gtgtttgatc cgttgcgtta tccggatatc ggtcgccagc tttctccgga cgcgtgggat
     9181 gatgtttcgc aggagtaatc acaactatcg atcaactcat tctctttttt tgctaaagtc
     9241 ggcataaatt tcctgcaagg actggatatg ctgattctta tttcacctgc gaaaacgctc
     9301 gattaccaaa gcccgttgcc caccacgcgc tatacgctgc cggagctgtt agacaattcc
     9361 cagcagttga tccatgaagc gcggaaactg acgcctccgc agattagcac gctgatgcgc
     9421 atcagcgaca aactggcggg tatcaacgcc gctcgctttc atgactggca gccagatttc
     9481 acgccggaga atgcccgcca ggcgattctg gcattcaaag gtgatgtcta caccggcttg
     9541 caggccgaaa ccttcagcga agacgatttc gattttgccc aacagcattt gcgaatgctt
     9601 tccggcttgt atggcgtact gcgcccgctc gatttaatgc agccttatcg tctggaaatg
     9661 gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc aattctgggg agatatcatc
     9721 accaacaagc tgaacgaggc gctcgcagca caaggcgata atgtggtgat taatctggcg
     9781 tcagatgagt attttaaatc ggtgaagccg aagaaattga atgccgagat tatcaagccg
     9841 gtgttcctcg atgagaagaa cggcaaattt aagatcatca gcttctacgc taagaaagca
     9901 cgcggtctga tgagtcgttt cattattgaa aatcggctga ccaaaccaga gcaactgact
     9961 ggttttaata gcgaaggtta cttctttgat gaagcttcct ccagcaacgg cgaactggtg
    10021 tttaaacgct acgagcagcg ttaagtttgc agtcaatgcc ggatggcaat ccggcattga
    10081 tatttagcga tgatgtttgt caggccccgg acgatgatcg ccacgatgat cattgtgctt
    10141 gttatggcgc ggcgatggcg gcggtccata tgggtgccag cgattgcctc gccattcata
    10201 atgttgtttc caccagccgt ggtcgcgcca gtgaccgcca tcccagtaat agccacgatt
    10261 atcacgatcg cctatctgta attttactga cgggactaac gtcatttccg cagcctgcgc
    10321 agccatggga gcgaccagaa ccagggaaag tgcgagtacg atagattgca tctttttcac
    10381 aggttactcc ttttatcatt gcccgttatg ggtcgatatc cggagattac gcgatgagaa
    10441 cggcagttgt tattctccgc aatcctaatc tctaagttac cgcatttatt gagcatttct
    10501 cctttttccc tgtcgttttt ctccataatt tcttcataaa aaagccgggc tgcataaaag
    10561 caaacccggc ctgattgaga taatgaatag atattactga tgattcatca tcaatttacg
    10621 caacgcagca aaatcggcgg gcagattatg tgaaagcaag ggtaaatcgg cacgttctgc
    10681 cagctctttt ggcagatcca acgtttcacc gagaatcgct tccacgctct ctttaaattt
    10741 cgccggatgc gcggtgccga ggaacaagcc atattcgcct ggatgcaact ggtcacgcag
    10801 tgcacgatac gctacggcag cgtgcggctc cgaagtgtag cccagttctt ttaactcacg
    10861 cattgtctgt tgcgtggttt catcatccac ggctgcataa cccagctctt tcagttgcca
    10921 gattttgcgg cggaacaact cttccacacg cggccagttg ttcggctgac tcacgtccat
    10981 cgcgttggat aacgtcgcct gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg
    11041 tggcacggta tcgttcacgt tggtcgcagc aataaaacgt ttcaccggca gaccgagtga
    11101 cttcgccagc agacccgccg tcaaatcgcc gaagtttccg cttggcaccg agacaaccag
    11161 ctggttgcgc gcctcctgcg gcagctgcgc aacagcttca aagtagtagc aaatctgcgc
    11221 cagcaaacgg ctgatgttaa tcgagttagc tgagtttagc cccagcgcca ctttcagttc
    11281 ttcatcatca aacgcctgct tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc
    11341 aacagtttca atattgccgc ccaatgtaca gaacagtttt tcttgcagtg gactgatttt
    11401 gcctcgtgga tagaggataa ccactttcac attcggcaaa ccgtagaaag catgagccac
    11461 tgccgctccg gtatcaccgg aggtcgcggt cagaatggtc actggcttat cgcccgcaat
    11521 atgggtcagc atttgtgcca taaagcgacc gccgaaatct ttaaatgcca gcgttggccc
    11581 gtggaacaat tccagacaac cgacatcgct ttcaacattg gcgaccggag ccgggaagac
    11641 aaacgccgcg cgtacgcgct cttccaggat ttcctgcggg atttcatcac caataaacgc
    11701 cgagaggatc ttcgcactgc gggtgacaaa atccagcttc agcatctcat caatttcagt
    11761 caggctgaat tccggcaggt cgtgcgggaa aaacagcccc tgatttttgc ccaacccctg
    11821 ggttacggct tgcgcaaagc tgacctgctc attgtgatct ttcagattgt agagtttcat
    11881 ttagttttcc agtactcgtg cgcccgccgt atccagccgg caaatatgaa caaaaccttc
    11941 ctgattttgc aggtagttct tacccaacca gtcggcaacg cgctgggcgg tatccggctt
    12001 gtcacacaga gcgaacaaag tcgggccgga gccggagata ccgctcgcta ccgcgccgat
    12061 ttccgcgacc gcctgccgcg cctgccggaa gcctggcagt aaccgttcac ggtagggttc
    12121 agcgataaca tctttcatca gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg
    12181 aataaagcct gccagatgtc gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg
    12241 taaaatagcc ctggcttctg ccgtcgagac tttaatcccc ggatacgcca gcacccacag
    12301 ccactcatca aaccctggca cttgctggct gatgatgtcg ttttcttcga tcatcaactg
    12361 cataccaccg agaaaacacg gtgccacgtt gtcgtaatga atgctgccgg agatacgccc
    12421 ttccagctcg cccatcaaag ccagcaaacg ggtgtcatta agcggcttgc cgcagtgttc
    12481 attcatcgcc atcagcgccg cgaccactga gcaggcgctg gagcctaagc ccgagccgat
    12541 cggcatattc ttttccaggg tcatcgccac tggaatttgc ttacccagct cctggcaaaa
    12601 acgctcccaa cactgataaa cgatattttc ccgaggttct gacggcagct tatcggcaaa
    12661 gcgtccgagg ttattgagac tgaatgtctc tgccgcctca accgtgacta catctccgag
    12721 caatgcacca tcaacgggtg tcaccgccgc cccgagcaca tcgaacccga cgctcatatt
    12781 ggcactggaa gccggggcat aaactttaac catgtcagac tcctaacttc catgagaggg
    12841 tacgtagcag atcggcaaag acaccggcag ctgtaacgtc attgcccgca ccatatccgc
    12901 gcagcaccaa cggcagcggc tgataatagt ggctatagaa ggccagggcg ttttcgccgt
    12961 ttttcacttt gaacagcgga tcgttgccat ccacttcagc aatcttcacg cggcagacgc
    13021 catcttcatc aatattgcca acatagcgca aaacttttcc ttcatcacgg gccttcgcca
    13081 cacgagcggc aaagagatca tcgagctgtg acagattcgc cataaaagcg gcaacatcac
    13141 cctcggcgtt aaactcagcg ggtagcacag gttcaatttc aatatccgcc agctccagtt
    13201 cacgtcccgt ttcacgagcg agaatcaata gtttacgcgc cacatccata ccagaaagat
    13261 catctcgcgg atccggttcg gtataaccca tttcccgcgc caacgtggtc gcctcggaga
    13321 aactcatgcc ttcgtctaac ttgccgaaga tataagaaag cgaaccagaa agaatgccgg
    13381 agaacttcat caattcatca cctgcattga gcagattttg caggttctca ataaccggta
    13441 atccagcccc aacgttggtg tcatagagga atttacgccg cgatttttcc gccgcatgac
    13501 gcaacagatg gtagtaatcc atcgacgagg tgttagcctt tttgttcggc gtgacgacgt
    13561 ggaaaccttc gcgcaggaag tcggcatatt gatccgccac tgcctggctg gaggtgcagt
    13621 caacaatgac cgggttcagc agatgatatt ctttcacgag gcgaattaag cgcccgagat
    13681 taaacggctc tttggcttgc gccagttctt cctgccagtt ttccagatta aggccatgta
    13741 cattggtgag cagagccttc gagttggcaa cgccgcagac acgtaagtcg atatgtttat
    13801 tcttcaacca gctttgctga cgcttcagtt gctccagcag cgcaccgcca acgccaccga
    13861 cgccaatcac aaacacttcg ataacctgat cggtattgaa cagcatctga tgagtaacgc
    13921 gcacgccagt ggtcgcatca tcgttattta ccacgacaga gattgagcgt tcagaagatc
    13981 cctgagcaat ggcgacaatg ttgatattgg cgcgggccag cgcggcaaag aatttcgccg
    14041 agatcccacg caaggtgcgc ataccatcac ctaccaccga gataatggcc agccgttccg
    14101 tcaccgccag cggctccagt aagccttctt tcagttccag gtagaactct tcctgcattg
    14161 cccgttcagc tcgcacacag tcgctttgtg gaacgcagaa actgatactg tattcggaag
    14221 atgattgcgt aatcagcacc acggaaatac gggcgcgtga catcgctgca aagacgcgcg
    14281 ccgccatgcc gaccatccct ttcatccccg gaccagaaac gctgaacatt gccatgttat
    14341 tcagattgga aatgcccttg accggtaatt cgtcttcatc acggctggca ccaatgagcg
    14401 tacctggtgc ttgaggattt ccggtatttt taatcaggca agggatctgg aactgggcga
    14461 tgggggtaat tgtgcggggg tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt
    14521 cctggtagga catcgacttc aacaacctcg catcgggcac ctgacgcggg tcgcaggtat
    14581 agaccccgtc aacgtccgtc caaatctcgc aacaatcggc gcgtaaacag gcagccagca
    14641 ccgccgcgga gtagtcggaa ccgttacgtc caagcaccac cagttcgcct ttttcattac
    14701 cggcggtgaa acctgccatc aacaccatgt gatcagccgg aatacgactt gccgcaatac
    14761 ggcgggtgga ctcagcaata tcgacggtag attcgaggta atgccccact gccagcagtt
    14821 tttcgaccgg atcgataacg gtaacgttgt gaccacgcgc ttctaacacg ccggccataa
    14881 tggcgatcga cattttctcg ccacggcaaa tcagcgcagc gttgatgcta tccgggcact
    14941 gccccaacaa actaatgcca tgcaggacat gttttatttg ggcaaattcc tggtcgacga
    15001 aagttttcag ttgcgccagc gggaatcccg gctgagcggc ggcgagtccc gtcagaagtt
    15061 cggcaaaaat acgttcggca tcgctgatat tgggtaaagc atcctggccg ctaatggttt
    15121 tttcaatcat cgccaccagg tggttggtga ttttggcggg ggcagagagg acggtggcca
    15181 cctgcccctg cctggcattg ctttccagaa tatcggcaac ccgcagaaaa cgttctgcat
    15241 ttgccactga tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg ttacctttgg
    15301 tcgaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca
    15361 gcccgcaccg ttacctgtgg taatggtgat ggtggtggta atggtggtgc taatgcgttt
    15421 catggatgtt gtgtactctg taatttttat ctgtctgtgc gctatgccta tattggttaa
    15481 agtatttagt gacctaagtc aataaaattt taatttactc acggcaggta accagttcag
    15541 aagctgctat cagacactct ttttttaatc cacaccgaga catattgccc gttgcagtca
    15601 gaatgaaaag ctgaaaaata cttactaagg cgttttttat ttggtgatat ttttttcaat
    15661 atcatgcagc aaacggtgca acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg
    15721 ttgtaaccag tcgaccagtt ttatgtcatc cgccactgcc agagtcgtca gcagtgccat
    15781 ggctcgttcg cgtaaagctt gcagttgatg tttgtctgcc gttgtgtcac ttttcgccgg
    15841 ttgttgtatt aatgttgcta attgatagca atagaccatc accgcctgcc ccagattgag
    15901 cgaaggataa tccgccacca tcggcacacc agtaagaacg tcagccaacg ctaactcttc
    15961 gttagtcaac ccggaatctt cgcgaccaaa caccagcgcg gcatggctca tccatgaaga
    16021 tttttcctct aacagcagca ccagttctac tggcgtagcg tagtaatgat atttcgcccg
    16081 actgcgcgca gtggtggcaa cagtgaaatc gacatcgtgt aacgattcag ccagtgtcgg
    16141 gaaaacttta atattatcaa taatatcacc agatccatgt gcgacccagc gggtggctgg
    16201 ctccaggtgt gcctgactat cgacaatccg cagctcgcta aaccccatcg ttttcattgc
    16261 ccgcgccgct gccccaatat tttctgctct ggcgggtgcg accagaataa tcgttatacg
    16321 catattgcca ctcttcttgc tcaaataacc gcgaaccggg tgaccactgt caacttatta
    16381 cgcggtgcga atttacaaat tcttaacgta agtcgcagaa aaagcccttt acttagctta
    16441 aaaaaagcta aactatttcc tgactgtact aacggttgaa ttgttaaaaa atgctacata
    16501 tccttatgtt tacttaggat aattttataa aaaataaatc tcgacaattg gattcaccac
    16561 gtttattagt tgtatgatgc aactagttgg attattaaaa taatgtgacg aaagctagca
    16621 tttagatacg atgatttcat caaactgtta acgtgctaca attgaacttg atatatgtca
    16681 acgaagcgta gttttattgg gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat
    16741 ggttaggatg acagccgttt ttgacactgt cgggtcctga gggaaagtac ccacgaccaa
    16801 gctaatgatg ttgttgacgt tgatggaaag tgcatcaaga acgcaattac gtactttagt
    16861 catgttacgc cgatcatgtt aatttgcagc atgcatcagg caggtcaggg acttttgtac
    16921 ttcctgtttc gatttagttg gcaatttagg tagcaaacat gcagaccccg cacattctta
    16981 tcgttgaaga cgagttggta acacgcaaca cgttgaaaag tattttcgaa gcggaaggct
    17041 atgatgtttt cgaagcgaca gatggcgcgg aaatgcatca gatcctctct gaatatgaca
    17101 tcaacctggt gatcatggat atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg
    17161 aactgcgcga gcaggcgaat gttgcgttga tgttcctgac tggccgtgac aacgaagtcg
    17221 ataaaattct cggcctcgaa atcggtgcag atgactacat caccaaaccg ttcaacccgc
    17281 gtgaactgac tattcgtgca cgcaacctgc tgtcccgtac catgaatctg ggtactgtca
    17341 gcgaagaacg tcgtagcgtt gaaagctaca agttcaatgg ttgggaactg gacatcaaca
    17401 gccgttcgtt gatcggccct gatggcgagc agtacaagct gccgcgcagc gagttccgcg
    17461 ccatgcttca cttctgtgaa aacccaggca aaattcagtc ccgtgctgaa ctgctgaaga
    17521 aaatgaccgg ccgtgagctg aaaccgcacg accgtactgt agacgtgacg atccgccgta
    17581 ttcgtaaaca tttcgaatct acgccggata cgccggaaat catcgccacc attcacggtg
    17641 aaggttatcg cttctgcggt gatctggaag attaatcggc tttaccaccg tcaaaaaaac
    17701 ggcgcttttt agcgccgttt ttatttttca accttatttc cagatacgta attcatcgtc
    17761 cgttgtaact tctttactgg ctttcatttt cggcagtgaa aacgcatacc agtcgatatt
    17821 acgggtcaca aacatcatgc cggccagcgc caccaccagc acactggttc ccaacaacag
    17881 cgcgctatcg gcagagttga gcagtcccca catcacacca tccagcaaca acagcgcgag
    17941 ggtaaacaac atgctgttgc accaaccttt caataccgcc tgcaaataaa taccgttcat
    18001 tatcgcccca atcagactgg cgattatcca tgccacggta aaaccggtat gttcagaaag
    18061 cgccagcaag agcaaataaa acatcaccaa tgaaagcccc accagcaaat attgcattgg
    18121 gtgtaaacgt tgcgcggtga gcgtttcaaa aacaaagaac gctataaaag tcagtgcaat
    18181 cagcagaatg gcgtacttag tcgcccggtc agttaattgg tattgatcgg ctggcgtcgt
    18241 tactgcgacg ctaaacgccg ggaagttttc ccagccggta tcattgcctg aagcaaaacg
    18301 ctcaccgaga ttattagcaa accagctgct ttgccagtgc gcctgaaaac ctgactcgct
    18361 aacttcccgt ttggctggta gaaaatcacc taaaaaactg ggatgcggcc agttgctggt
    18421 taaggtcatt tcgctattac gcccgccagg caccacagaa agatcgccgg taccgcttaa
    18481 attcagggcc atattcagct tcaggttctg cttccgccag tccccttcag gtaaagggat
    18541 atgcacgccc tgcccgcctt gctctaagcc ggtgccgggt tcaatggtca gcgccgttcc
    18601 gttaacttca ggtgctttca ccacaccaat accacgcgca tccccgacgc taatcacaat
    18661 aaatggcttg cctaaggtga tatttggcgc gttgagttcg ctaagacgcg aaacatcgaa
    18721 atcggctttt agcgttaaat cactgtgcca gacctgaccg gtataaatcc ctatcttgcg
    18781 ttcttccacg ttctgattgc catcaaccat caatgactca ggtaaccaaa aatggataaa
    18841 acttcgtttc cgctccacgg ttttatcctc ttcctgcacc gtataaagct cggtcacagg
    18901 aatagcgatg agcggcccaa cgagtttttg cggcccgctg gtactttggc gaatcgcatc
    18961 ttccacatcg ctacggtaat cagcacgttc gacaatcacc tgccgaatca gcattatcgg
    19021 aatcaacaac agcaatactg caccaaacag ggtggtcatt ttccagaaca ggggggattt
    19081 caacatagtc ttctcctttg caatggcagc aggatacgaa ggctatgtgg gaagaatttg
    19141 aagctatgtg aagtgacggt gaagtcgaag cgaagccagc acgccacctt cctgcacatt
    19201 gcgcagcgtg acttcgccgt taaacaaacg ggcgacctca ctgacgaacg ccaaccccag
    19261 accgctgctt ttttgcccat ttgcacgagg caaagagtaa aagcgttcaa aaatacgtga
    19321 aagcgcgtag tcaggaatac cactaccggt atccagcacc ttaagcgtga cgtgttcctg
    19381 ctccacttcg gcgcttagcg tgatgcaacc gctctcgggg gtaaaatcga tggcgttatc
    19441 cagtaaattc cccagagcct gctccagtaa cgccggttca gcagcaacgt taacctcggt
    19501 gggcgtaaca tgtagagtga tgtttttttc tgccaactgc acggtgcgcg cttcgctgac
    19561 gcggcggaat aatgccgcca catcaacagc agttagaacg acttcctgac gattctccag
    19621 tcttgcctgg cgtagtaacg tttctaccaa tgcctgcata cgcgcatttt gcgtcagaat
    19681 gttgtcagta aaacgagcca ccacttccgg cggcggacct tcgcgtaaaa tttccgccgc
    19741 tccacgaatc gccgccagtg ggctttttag ctcatgagtt aatgcgtaaa catactgctc
    19801 aatatagttt ttcccttcca gcttcacgcg catactttcc agcgcctgcg cgagtttacg
    19861 cagctcgcta ctaccgagat cggggagagg aacgggctta ttgtcagtga cggaatcagc
    19921 atagcgagtg agccgggcaa tggagcggtt gatccaccaa accatgcctg cgccaatcac
    19981 cagtgcaatc cccaacaaaa tggcgctggc ccataaaatt cgccgctcgc tgcgtttaat
    20041 gaccggagcc atcgccgcgt tcggtttgcc tacgctcaaa acgccaataa gccgcgagcc
    20101 gtccataatc ggcgcggcaa catacatcac cgaactttcg ggatcggtag gattttgcaa
    20161 cgtgctgcgc gcaccatact gaccacgcaa cgttagccag acgtcattcc agcgcgaata
    20221 atcctgtcca acggctttat ttgccgaatc gaacaatact ttgccctgcg catcggtcat
    20281 atagacatga tattcattgc gcactttgtt aatgccaccg atattggcgc gaaacgggcg
    20341 atgctgtagc tgattaaacg cctgcgccag ttgcccatgc gttgtgtccc cagagagcaa
    20401 atcgggacgc gccagctccg ccagcaacgt tgcggtgtcg atcaacgtcc cctccgttgc
    20461 tcttcgcacg cccggtttaa cttctttgac aaaaatggcc agtacgaacc aagctgccac
    20521 cgccaccagt aaaaaatagc ccagcaacaa ccgcatgccg atacgcatta caggcccctc
    20581 aggctatatc ccatgccgcg atgagtatta atcggtgaaa gatcggggtt gatggcgcgc
    20641 agcttggcac gcagcgtttt aatgtgggta tcgacggtgc gatcgtaggt gtcctgcgca
    20701 tcttcccaca cgctatccat cagttgctgg cgggaccaga cgcggcctgg cgacttgagt
    20761 aacgtcttca gcaataaaaa ctcataccgc gtcagcgtta atggcgtgtc aaaccagctg
    20821 atctgcgccg cgggttcatt caattcaaaa tgaccaatac ggatgacggg agacggcgtc
    20881 gagaacttct tcacccgacg cagtaaggtg cgcaccctgg cgcacacttc gcggggtgaa
    20941 aacggtttag ccacatagtc gtcagcgcca atttccagcc caagcaggcg atcgacctct
    21001 tcacttcggg cggtcaggaa cagtacaggt aacgccggat ggagcgtcag taactggcgg
    21061 cacaattcaa agccactgat atcaggcaaa ccaacgtcga gaatcatgac atcgggaacc
    21121 tgctggcgag ctttatccag caccggcaag cctcgctcaa agacctcgac ggcaaaacct
    21181 tcctgctgca acatgtagac cagcgtgtcg gctatccctt gctcatcttc cactaaccag
    21241 accgtttccc gttgcataaa tcgcctctgt tattgccgcc acggcatgac aggaaccgcg
    21301 ctaatcgcgt ttttcggcga accttctaca actttgtcgg agtaagccag ataagcgagc
    21361 gcgttgcgtt tagcgtcata aaagcgcacg acctgtaacg acttaaagac cagggacgtg
    21421 cgttttttga atactacctc gccctgagct ttgccgtttt taatacgatc cgacagttca
    21481 atcggcccga cttgctgaca agaaatggcc gcatcggagg tatcttctgc caggcccaat
    21541 ccccctttaa taccaccggt tttcgcccga ctcacataac aggtgacatt tttcacatcg
    21601 ggatcgtcaa aggcttccac aacaattttg tgatccgggc cgatcatttt aaatacggtg
    21661 tcgaccgaac caatctcttc tgcatgagcc aatggcccca gcattatcag gcttaaagac
    21721 aggatcaaat gcttgtattt catattgtta ccatttttat ctattacacg agcaattagt
    21781 tcgtcacggt aaataacata ttgattaaga acaaaatctc aatactttta tttccgtcaa
    21841 gccctaaaac atactctact aagaaaaaaa cactgaatgc taaaacagca aaaaatgcta
    21901 ttatccaatt acctgatgtc aggtgctcgt tgttgaaagg atgaggatat tttatggatc
    21961 aggccggcat tattcgcgac cttttaatct ggctggaagg tcatctggat cagcccctgt
    22021 cgctcgacaa tgtagcggcg aaagcaggtt attccaagtg gcacttacag agaatgttta
    22081 aagatgtcac tggccatgct attggcgcgt atattcgtgc tcgtcgtttg tcgaaatcgg
    22141 cggtcgcact acgcctgact gcgcgtccga ttctggacat cgcgctgcaa taccgcttcg
    22201 actctcaaca gacatttacc cgcgcattca agaagcagtt tgcccagact cctgcacttt
    22261 accgccgttc tcctgaatgg agcgcctttg gtattcgccc gccgctacgt ctgggtgaat
    22321 tcactatgcc agagcacaaa tttgtcaccc tggaagatac gccgctgatt ggtgttaccc
    22381 agagctactc ctgttcgctg gagcaaatct ctgatttccg ccatgaaatg cgttatcagt
    22441 tctggcacga ttttctcggc aacgcgccga ccattccgcc ggtgctctac ggcctgaatg
    22501 aaacgcgtcc gagtcaggat aaagacgacg aacaagaagt attctatacc accgcgttag
    22561 cccaggatca ggcagatggc tatgtactga cggggcatcc ggtgatgctg cagggcggcg
    22621 aatatgtgat gtttacctat gaaggtctgg gaaccggcgt gcaggagttt atcctgacgg
    22681 tatacgggac gtgcatgcca atgctcaacc tgacgcgccg taaaggtcag gatattgagc
    22741 gatactaccc ggcagaagat gccaaagcgg gagatcgccc aattaatctg cgctgtgaac
    22801 tactgattcc gatccgtcgt taacgctgca gctcatctaa tgcaggggcg tccagatgcg
    22861 agatgtcccc tgcggtttca acgacccagc cagacgccag ccacaggctt tcctgataat
    22921 ccacgcgcga aatagaacag ttacgcagac gtaagcggcg ctctgcccat gctggtaatc
    22981 cgagaatcgt actcaccaga catcccagtg caataccatg gctcaccagc agcggtcggc
    23041 ttccctgcgg taagtcccgg caagattcca gtgccgcatt gacgcgatcg ctgagctcct
    23101 gcattgactc gccttcagga atacgcccgt caacggtgcc attgaccagc tgccgacgcc
    23161 agttctcttc ttcttcggtg agtgaatcga tatttcttgt ttccagtaca cccatattta
    23221 attcacgcag gcgagaatca aagatgatat cacagccgca ggcctgggcg atgatttccg
    23281 ccgtgcgccg ggtccgtcct aaatcgctac tgatgatatg cgtaatgcca agctctttgg
    23341 cacgggttgc cacctgcatc gcctgttgct cacctttggc ggtcagcggg ctgtcagact
    23401 ggccctgaat acgtcgctcg gcgttccact gcgtttcacc gtggcggact aggtatacct
    23461 gtaacatgct ttttttccgt atactgctgt aacgttaatt ttctgagtaa tgctgattat
    23521 gcaccaagtt gtctgtgcga ccaccaatcc cgctaaaatt caggccattc tgcaggcatt
    23581 tcacgagatc ttcggcgaag gatcctgcca tattgcatcc gttgccgtcg agagcggtgt
    23641 accggaacaa ccctttggca gtgaggaaac gcgcgctggc gcacgaaatc gggtagcaaa
    23701 tgcccgccgt ttacatccag aggctgattt ttgggtggcg attgaagcag gcattgatgg
    23761 cgacagcacc ttcagttggg tggtgattga aaacgccagc cagcgcggcg aggcgcgttc
    23821 ggcaactttg ccgttacctg cggtgattct ggaaaaagtg cgtgaaggcg aggcgctcgg
    23881 tcccgtgatg tcacgttata ccggtattga tgagattggt cgtaaggaag gggcgatcgg
    23941 cgtgtttacc gccgggaaac ttacccgcgc cagcgtctat caccaggcgg tgatcctggc
    24001 gctgagtccg tttcataatg cggtgtatca gtgatctttc agcaacacct cttccagcca
    24061 ctggcgcagc tccacgggcg cggctttcag gctgttagat ccacgcgtaa tcgtcgcgat
    24121 gcccgcgccg agttcatttt ttaactcacg ctggctcatt tcgccgcgca acagctcttc
    24181 gacaatacgc acgcgagtcc ccaacgcttc gcgctcatct ggcgtcagca tcaggtttaa
    24241 caacggtaaa tggagatcgt tttggtaggc attcttaagc aggtcgacaa aacgtaacca
    24301 ctcctggtga cgctgttctg ccatcgctgc tgaatagggt gattgttggg ccataatatg
    24361 tcgccattgt tagcggggga agcaaaatgc ctcccccggt tgtactcgct aaagagtacg
    24421 atagcatatc ataaacgtgc ggatcagtaa cgacgtcccc attccgtggc gctcatcaac
    24481 gtcggtttat cccccatgaa atagcggtag taagcgtcat aagccagcac gttcttcaca
    24541 taaccgcgcg tctcggagaa cggaatactc tcgacaaatg ccacagcgtc gatacgcccg
    24601 gcgctgttgc caagccaggt tcgcacccgt ccaggtccgg cgttataagc tgctgaggag
    24661 aaaatacggt tattgccaaa ctgctgataa acatattgca ggtaactggt gccaatgttg
    24721 atgttcgttt ccggatccag caattgccca gggctgctat aaccaggaat agagaacatc
    24781 ttcaccgtat gggtcgccgt gcccggcata atctgcatca ggccgctggc ccctaccggt
    24841 gatttcactt tcggattcca ggcgctctcc tgacgggcaa tcgccatcgc atagctttgc
    24901 gggatctcct tcccactggt gtaacgtttg aaaagatcgt tgtaggccag cgggaatctc
    24961 tcttccagat gatcccaaag cttcccggcg atcgttgcct gaacgctaag ttcccaccat
    25021 tggttattga aagcataccg agccagttgc gcctgctctg tttttgactt gctcttcacc
    25081 agattggccc actcgctacg cgcggtatta tcgagattcc agtacatcaa ctcgcgcacg
    25141 cgcgccatct ccggcccctg agtcagggcg ctgtcaacat tctgcggcgc tttatcaatc
    25201 ttcagctcat actcttcacc gatgcgttgt gctgcaacca tcgggtagaa accacgctgt
    25261 tgcatgagtt gatgcaaaat ctcttttgct tcagcttcac gtccgcgttc cagcagtaaa
    25321 tccgcctgcc agtaacgcca ttcatctttc tctttcgcct ccatcggcaa acgcgccagc
    25381 caggtattca ggccgcgacg atcgccggtg ccaagcgcca ttcgcacgcg ccgttcaata
    25441 agcgaagtag attgcgaacg cataatggcg tcatcgcgcc atttcgcctg ctcatcggtg
    25501 acatcattgc ccatcaaacg ccaggcgacg atatcgcgca gctcctgaat ctgatcttca
    25561 ttaagctgct gcgcctgggc aagcgacggg atcatcagcc gtgcattttc agcatcctgc
    25621 cgcgccacac tggcaaacgc caccgccgcc atttgacggg taaaatcggt cgctccggtt
    25681 gtacgcgcga aggtcagtac cgtattaggg ttgttcgcca gtgaaatgat tgccgaggcg
    25741 atagtctggt aatcggcagg catctgccct gccagcactg ttacgagtcc agtgttaccc
    25801 gctttcatcg ccagacggat acgctctaaa tacgccagcg gatcttgttt acctgacgca
    25861 cgccagacgc taaataactt gtcacaggcg ttaggctggc tcttgccggt tagccacagc
    25921 tctttcgccc cttgccaggc ttcttcactc tgcccggtgt tccatttcgc atagtagtaa
    25981 ttacattgcg cttcggtagt tccgggcttt tccgggctaa aggctaacaa gccacgccag
    26041 tcttcacgcc gcgccagttc attgacgaaa cgagattgca gcgtgcgagc gggaggaagc
    26101 gtggggttag cgcgaacaaa gttagtgacc gtcaccgccg gttgattcat caggtcatcg
    26161 gtgatctgac ggtattccag gtagggataa agcggataat ctttcagtcc aggcatcatt
    26221 tgttcgacca catccatttg tcgattatcc caggcctgct tgatttgggc gtagcggcta
    26281 cgctgctcat ccagtgagtc ggctcgcgcc acgctgctga ccgtcagcag acagacaccg
    26341 gcagccaaca gccgccaggt aacttgtttg gctttttcca caagcgcatc ctctaagtgt
    26401 aaatacatca atgcagcgtc atgccgcgta atagatttat gctaaccagt cattgccgtt
    26461 tacgccacgt tacggacact tttttacttt tatactctaa ataattcgag ttgcaggaag
    26521 gcgataagtg agtgagtccc caggagctta cagaagtaag tgactggggt gagcgagcgt
    26581 aagcgcagca catgcaactt gaagtatgac gagtatactg cgagggtgat cgaccggctg
    26641 ggattagata aggaaacagg ctacacttcg cctttgaaaa ctcatcatca cgataaacat
    26701 agaggcgaag tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg gcaaagttgt
    26761 tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt ttcttccctg gggcaaaaat
    26821 tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg ctgcgcatta tggcgggcat
    26881 tgataaagac atcgaaggcg aagcgcgtcc gcaaccggat atcaagattg gttatctgcc
    26941 gcaggaaccg cagttgaacc cggaacacac cgtgcgtgag tccattgaag aagcggtttc
    27001 tgaagtggtt aacgccctga aacgcctgga tgaagtgtat gcactgtacg ccgatccgga
    27061 tgccgatttt gacaaactgg ccgctgaaca gggccgtctg gaagagatca ttcaggctca
    27121 cgacggtcac aacctgaacg ttcagctgga gcgtgcggcg gatgcgctgc gtctgccgga
    27181 ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtgg cgttgtgccg
    27241 cctgctgctg gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc acctggatgc
    27301 cgaatccgtg gcctggctgg aacgcttcct gcacgacttc gaaggcaccg ttgtggcgat
    27361 tacccacgac cgttacttcc tcgataacgt tgcgggctgg atcctcgaac tggaccgcgg
    27421 tgaaggtatt ccgtgggaag gtaactactc ctcctggctg gagcagaaag atcagcgtct
    27481 ggcgcaggaa gcttcacaag aagctgcgcg tcgtaagtcg attgagaaag agctggagtg
    27541 ggtacgtcaa ggtactaaag gccgtcagtc gaaaggtaaa gcacgtctgg cgcgctttga
    27601 agaactgaac agcaccgaat atcagaaacg taacgaaacc aacgaactgt ttattccacc
    27661 tggaccgcgt ctgggcgaca aagtgctgga agtcagcaat ctgcgtaaat cctatggcga
    27721 tcgtctgctg attgactccc tgagtttctc gatcccgaaa ggggcgatcg tcgggatcat
    27781 cggtccgaac ggtgcgggta aatcgacgct gttccgtatg atctctggtc aggaacagcc
    27841 ggacagcggc accatcactt tgggtgaaac ggtgaaactg gcatcggttg atcagttccg
    27901 tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc tggatatcat
    27961 gaaaatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta actttaaagg
    28021 ggttgatcag ggtaaacgcg ttggcgaact ctccggcggt gagcgcggtc gtctgcatct
    28081 ggcgaagttg ctgcaagttg gcggcaacat gctgctgctc gacgaaccaa ccaacgacct
    28141 ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg gctgcgcgat
    28201 ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc tggattacca
    28261 ggatgagggt aaagttgagt tcttcgaagg taactttacc gagtacgaag agtacaagaa
    28321 acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc aagtacaagc gtattgcgaa
    28381 gtaatgcgta aaatgccgga tgcggcgcgc cagcatcgca tccggtatta gttaaatgat
    28441 taatgggagg caagttcccg gaaaaagtca ggattttctt taattgtggc aagtactttt
    28501 gcagccaatc ccgtaggatt tcttcgcttc gattcccagc ttttaatggt atccacactg
    28561 gttcccagag cttttgccat ctcactttgc gaaacgttga gttgctcacg aatagcttta
    28621 acatccgcaa tttcgtagcg ggtcacccgt gcaggttctt ttaaaccttg tttaatctcg
    28681 acagcctctt ccagagaggt tttcaactca tcaaaaaaac tcattttata tctcgccttt
    28741 gagcagtttc gtcaacttct ttagttgagc tttttccgta tctgtcagac tatctttggc
    28801 atttttcgga taggccataa tcaaataaat aatctcttcc gtcgccagaa agtaaattat
    28861 tctgacactg ccactttttc cctgtgaacc cgcagccatt cggactttac gcaatccacc
    28921 agtctgctga atgagatcac ctttatcggg agttccgatg agttcttttt gtaattcctt
    28981 taattcatca tcggttgcaa tctgcttgat ctgacgcgta aacattggcg tctctataaa
    29041 tattatcgcc ttacccatta cccctccctg gaaggtacat tgtacaccaa aatggagtac
    29101 taagtacact tttgaatcag taatcccact caacatctta taaagatgat tatctttgct
    29161 cccccatcat ctcccgcacc agctccacgc agcgcaggaa acggctgtca taatcgtctt
    29221 cttcaacccg cacaaattcg atattgttct cttccagcat ctccaccagc aagttctgga
    29281 actctttgcg atccaccgaa ctgccgaggc tgcgtaaacc atccgccacc caaggggtgt
    29341 tgttctccag caagataacc agatcaaaac ggtattcatc gatcagcgcc tgcacgaacg
    29401 gatgctcccg cccttcgtac tttttgcaga acgcctgggt agtgacaaaa tcagtgtcga
    29461 taaacgccac tttattggca tatttcactg caaaatcaat gtattgtgcg tgtcccagcg
    29521 cgattttatc gtagtcggaa tactgcaatg cgatctcatc accgccgagg tgtgaaaaga
    29581 cataatcgcg accatattcc cacgcactgg tggtattgaa gatattggca agtttgttta
    29641 ccagggtgga tttaccgctc gactcgccgc caaggatcgc cacggtacgc acaaaaaacg
    29701 gcttcacttc agtggggata tattcccagt agcggaacgg gttttcgcgg atctgcgcac
    29761 cgctgatact cataaaggtg cgtttcggat cgaccagcac cgtttcgatc cccagatgtt
    29821 ccatatactg tggcgcatcg gcttcttccg aggtgtagat caggtccggc tggatcccct
    29881 tttcagccat aaactttttg atgccgttgc tccatacatc ccagccgtgc ggatatggct
    29941 ccatgccctc ttcattgaaa gcatgaatac ggatattttt ctgatattta aaggtttgca
    30001 ataaccagcg cagacgatcc ggcaccgtgg gctgctgcga catggcgctg tcttcgaaca
    30061 acgcgcggtc gcgggtatcg tcaaagccca taatgatatg cagctcgtca acctggctac
    30121 aggcgcgctg gataaggtag atatgtccgg tatgcagcgg gtagaactta ccgaatacga
    30181 caccaatggt tttcttctgt cggggaaatt caagccccaa aaaacggtgc aacgcctcca
    30241 gcttttgcgc gctggggctt ttaattttgg cattcagtaa ctggcttaaa taccctttgg
    30301 tcataccgct ggcatcagcg acctgctgta gcgtgcagcc ctgttgcttg atggcagttt
    30361 tcaggtaatc aaatgacgac atatctccct ccgtatctct cattataagt cgtcgaacac
    30421 gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc atcccttccg gcgctttttt
    30481 cggcacgtta gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg cttcagaaat
    30541 tcgttcctgg ccgctgggca ctgggcggat ctcccctgcc agcccgactt caccaaacac
    30601 caccagatcc tgcggcagcg gtctgtcgcg caggctggaa accatcgcca gcagtaacgc
    30661 taagtcggcg ctggtttccg ttaccttcac gccgccgacc acgttaacaa atacatcctg
    30721 atcggccatt tgcaacccac cgtgacggtg cagcaccgcc agcaggattg ccagacggtt
    30781 ttgctccagc cctactgcga cgcggcgtgg gttcgccatc atcgagtgat cgaccagcgc
    30841 ctggatctcc accaacagtg gacgcgttcc ttcccacact accatcactg agctaccgga
    30901 ggtcacttca tcaccgcggc ttaaaaagat ggcggaaggg ttgctaactt cacgcagccc
    30961 ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt ttttatggct
    31021 gcgtaaggtg cgaaaacgag agtcggcatc gccatccaga agcaccgaac agtcgataca
    31081 gtgttccagc actttagggc cagccagcga gccatctttg gttacgtgcc caaccatgac
    31141 aatcgccacg ccgcgcgttt tggcgaagcg cgtcagataa gccgccgttt cacgcacctg
    31201 cgccacgctg ccaggcgatg actgaacatc cgccatatgc atcacctgga tggagtcaat
    31261 taccatcagc ttcggttgct cttcttcggc aatcaggcag atctgctcga tgctggtttc
    31321 cgacagcata ttaagattgt cagtcggcag gccaaggcga tgagcgcgca ttgccacctg
    31381 ttgcagcgac tcttcgccag tgacatacag cgttttcatc tgctgcgcca gtttgcacag
    31441 cgtttgcagc aacagcgtgg atttccccgc acctgggtta ccgccaatca gaatggcgct
    31501 tcccggcacc accccgccgc ctaatacgcg gtcgaactct ttaaatccgg tggaaaaacg
    31561 cggcagctcc tcaaggctga tgtcggaaag tttctggact tttgccactc cggcgctacc
    31621 ggcatagcca ctgagacgct cgttacgcgc caccgttggc gacgcagcaa gacgcacctc
    31681 ggtgatggtg ttccaggcat gacaggcgct gcactgcccc tgccagcgcg gataatcggc
    31741 cccgcattca ttacaaacaa aggcgcgttt tggagctttt gccacggtgt acctcgttaa
    31801 tgctgtgccg cccgcaggat gacgggcagg caattacttc tgattcaggc tgccagagag
    31861 gatgcagaat acccccatca ggtcagcgtg acggatggtg acttccgtct tttcattcac
    31921 ttttggcttg gcatggtagg caatccccag ccctgccgct ttgatcatcg gcaggtcatt
    31981 ggctccatcg ccaatcgcca cggtctgcgc cagcgggatt tcatactcct gcgccaggcg
    32041 ggtcagggtt ttcgctttat attgcgcgtc aacgatgtcg ccgatcacat tgccggtaaa
    32101 tttaccgtcc atgatctcca gttcattggc taccactgct gtcaggcgca gcttgtcgcg
    32161 caggtattca gcaaagaaag taaagccgcc ggaggcaatc gccactttcc agcccagcgt
    32221 ttccagcttg agtaccagct gcgttaagcc tggcatcagc ggcagatttt cacgcacctg
    32281 ttgcagaata ttggcgtcag cgcctttcag cgtcgccacg cggctgcgca ggctggcggt
    32341 aaaatcgagt tcgccgcgca tcgcccgttc ggttacctcc gccaccctct cgcccgttcc
    32401 ggccagtttg gcaatttcat caatacattc aatctggatg gcggtggaat ccatgtccat
    32461 caccagcaaa cccggcgtgc gcaggtgcgg gattttcccc agcggcgcga catccagctg
    32521 cgcttcgtgg gccaggcgcg tagcccgcgc ggtgagtgaa cctgccagac gaatcacctg
    32581 ataatcttcc acgcaccagg cggcaacaat caccatcgcc gcgcccagtt tgctctggta
    32641 ttgggtcaga cgttgtttat ccagcccacg accatacagc agccagccgc tacgacctgc
    32701 gtggtaatcc agtggcatca cttcatcacc acttaatgaa agaggcagac ccggccataa
    32761 agagacatct tcaggcaggt cgcaccaggt aatgttaggc attaaggctc ctgtaaaatc
    32821 gttcgaagca gggaaaataa cgcatgaggc taccttgtat ccattgcttc tggcaacatt
    32881 aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt ccggctgcat
    32941 cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg cgctgatgca gggagcgtca
    33001 tggtttagtc aaaaccacca gcgacagcgt aatccacagc tggaagaact ggcccgcacc
    33061 ctggcgcgtc aggtgacgct gaacgttgca ccgctgatgc gtaccgactc accggatgaa
    33121 aaacgcattc aggcgatcct tgatcagtta acggatgaaa gccgtatcct cgacgcgggc
    33181 gtgtatgacg aacaaggcga tcttatcgcc cgttctggcg aaagcgtcga cgtgcgcgac
    33241 cggttggcgc tcgacggcaa aaaagcgggc ggctatttta accagcagat tgtcgaacca
    33301 attgccggga aaaacggacc gctcggctat ctgcgcctta cgctcgacac ccatacgctc
    33361 gccaccgaag cccaacaggt ggataacacc actaacattt tacgcctgat gttgctgctc
    33421 tcactggcaa tcggcgtagt gctgacccgc acgctgctac agggtaaacg cacccgctgg
    33481 cagcaatcgc ccttcctgtt aaccgccagt aaaccggtgc cggaagagga agaaagcgag
    33541 aaaaaagagt gacccattac tacaagaaag gaaatcgtta tgtccacatt acgcctgctc
    33601 atctctgact cttacgaccc gtggtttaac ctggcggtag aggagtgtat ttttcgccag
    33661 atgccggcta cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt agtgattggt
    33721 cgcgcgcaga acccgtggaa agagtgtaat acccggcgga tggaagaaga taacgtccgc
    33781 ctggcgcggc gcagtagcgg tggcggcgca gtgttccacg atctcggcaa tacctgtttt
    33841 acctttatgg ctggcaagcc ggagtacgat aaaaccatct ccacgtcgat tgtgctcaat
    33901 gcgctgaacg cgctcggcgt cagcgccgaa gcttccgggc gtaacgatct ggtggtgaaa
    33961 accgccgaag gcgatcgcaa agtctcaggc tcggcctatc gcgaaaccaa agatcgtggc
    34021 ttccaccacg gcactttgct tctcaattcc gaccttagcc gcctggcaaa ctatctcaat
    34081 ccggataaaa agaaactggc ggcgaaaggc attacctcag tgcgttcccg cgtgaccaat
    34141 ctgaccgaat tgctaccggg gatcacccat gagcaggttt gcgaggccat aaccgaggcc
    34201 tttttcgccc attatggcga gcgcgtggaa gcggaaatca tctccccgga caaaacgcca
    34261 gacttgccaa acttcgccga aacctttgcc cgccagagta gctgggaatg gaacttcggt
    34321 caggctccgg cattctcgca tctgctggat gaacgcttta cctggggcgg cgtggaactg
    34381 catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tgtttaccga cagtctcaac
    34441 cccgcgccgc tggaagccct cgccgggcga ctgcaaggct gtctgtaccg cgcggatatg
    34501 ctgcaacagg agtgcgaagc gctgctggtt gacttcccgg aacaggaaaa agagctacgg
    34561 gagttgtcag cgtggatggc aggggcggta aggtaactcg ctgattaaaa tatccaggga
    34621 aggaaatatg atcgatctgg aaaaccagga acgggagatt atcaatttaa tgttcagcca
    34681 gggtatatcc tggctaaccg ccgtgcgtat caggcataaa ctatcacttg ctgaagtaag
    34741 taaaatgtta ggtatctcca ttaattctct aaaacagatc gaaaagacgg aacggcttag
    34801 ttcaaacatc aaaagcaaaa tggcaggaat ttatggttgt ccaccagagt tgctgatatg
    34861 cccttcc
//
LOCUS       DAZUCC010000023        34808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZUCC010000023 DAZUCC010000000
VERSION     DAZUCC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.023"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            169..1446
                     /gene="waaA"
                     /locus_tag="ACHY09_001015"
     CDS             169..1446
                     /gene="waaA"
                     /locus_tag="ACHY09_001015"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFS3884018.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1454..1933
                     /gene="coaD"
                     /locus_tag="ACHY09_001016"
     CDS             1454..1933
                     /gene="coaD"
                     /locus_tag="ACHY09_001016"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS3884019.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1972..2781)
                     /gene="mutM"
                     /locus_tag="ACHY09_001017"
     CDS             complement(1972..2781)
                     /gene="mutM"
                     /locus_tag="ACHY09_001017"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFS3884020.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGESCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2879..3046)
                     /gene="rpmG"
                     /locus_tag="ACHY09_001018"
     CDS             complement(2879..3046)
                     /gene="rpmG"
                     /locus_tag="ACHY09_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS3884021.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(3067..3303)
                     /gene="rpmB"
                     /locus_tag="ACHY09_001019"
     CDS             complement(3067..3303)
                     /gene="rpmB"
                     /locus_tag="ACHY09_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS3884022.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3520..4188)
                     /gene="radC"
                     /locus_tag="ACHY09_001020"
     CDS             complement(3520..4188)
                     /gene="radC"
                     /locus_tag="ACHY09_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS3884023.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4360..5580
                     /gene="coaBC"
                     /locus_tag="ACHY09_001021"
     CDS             4360..5580
                     /gene="coaBC"
                     /locus_tag="ACHY09_001021"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFS3884024.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRERGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5558..6016
                     /gene="dut"
                     /locus_tag="ACHY09_001022"
     CDS             5558..6016
                     /gene="dut"
                     /locus_tag="ACHY09_001022"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS3884025.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6123..6719
                     /gene="slmA"
                     /locus_tag="ACHY09_001023"
     CDS             6123..6719
                     /gene="slmA"
                     /locus_tag="ACHY09_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFS3884026.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6756..7397)
                     /gene="pyrE"
                     /locus_tag="ACHY09_001024"
     CDS             complement(6756..7397)
                     /gene="pyrE"
                     /locus_tag="ACHY09_001024"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS3884027.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7463..8179)
                     /gene="rph"
                     /locus_tag="ACHY09_001025"
     CDS             complement(7463..8179)
                     /gene="rph"
                     /locus_tag="ACHY09_001025"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS3884028.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8306..9169
                     /gene="yicC"
                     /locus_tag="ACHY09_001026"
     CDS             8306..9169
                     /gene="yicC"
                     /locus_tag="ACHY09_001026"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS3884029.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9391..10215
                     /gene="dinD"
                     /locus_tag="ACHY09_001027"
     CDS             9391..10215
                     /gene="dinD"
                     /locus_tag="ACHY09_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFS3884030.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVNIKQLENSVKITEKK"
     gene            10507..11124
                     /gene="yicG"
                     /locus_tag="ACHY09_001028"
     CDS             10507..11124
                     /gene="yicG"
                     /locus_tag="ACHY09_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFS3884031.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11121..12806)
                     /gene="ligB"
                     /locus_tag="ACHY09_001029"
     CDS             complement(11121..12806)
                     /gene="ligB"
                     /locus_tag="ACHY09_001029"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFS3884032.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGEETHRDAMMPPLNGAVIHPVAHTGVR
                     KMADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVR
                     LISAVPQTVSGPLANSTLQGEIFLKREGHIQKQMGGINARTKVAGLMMRQDDSDTLNS
                     LGVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNAWWKTKLPFV
                     TDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVARVAEVKAIQFAVGKSGKISVVASL
                     APVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPT
                     PPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRSLHQTHRFEHIF
                     SWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDER
                     SWSQLLLSTEQFWQQLPGTGSGRVRQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            13064..13687
                     /gene="gmk"
                     /locus_tag="ACHY09_001030"
     CDS             13064..13687
                     /gene="gmk"
                     /locus_tag="ACHY09_001030"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS3884033.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13742..14017
                     /gene="rpoZ"
                     /locus_tag="ACHY09_001031"
     CDS             13742..14017
                     /gene="rpoZ"
                     /locus_tag="ACHY09_001031"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS3884034.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            14036..16144
                     /gene="spoT"
                     /locus_tag="ACHY09_001032"
     CDS             14036..16144
                     /gene="spoT"
                     /locus_tag="ACHY09_001032"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS3884035.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16151..16840
                     /gene="trmH"
                     /locus_tag="ACHY09_001033"
     CDS             16151..16840
                     /gene="trmH"
                     /locus_tag="ACHY09_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFS3884036.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16846..18927
                     /gene="recG"
                     /locus_tag="ACHY09_001034"
     CDS             16846..18927
                     /gene="recG"
                     /locus_tag="ACHY09_001034"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS3884037.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18961..20166)
                     /gene="gltS"
                     /locus_tag="ACHY09_001035"
     CDS             complement(18961..20166)
                     /gene="gltS"
                     /locus_tag="ACHY09_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFS3884038.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20445..21836
                     /gene="xanP"
                     /locus_tag="ACHY09_001036"
     CDS             20445..21836
                     /gene="xanP"
                     /locus_tag="ACHY09_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFS3884039.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21957..23666
                     /gene="yicH"
                     /locus_tag="ACHY09_001037"
     CDS             21957..23666
                     /gene="yicH"
                     /locus_tag="ACHY09_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFS3884040.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGSWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVTGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(23710..24390)
                     /locus_tag="ACHY09_001038"
     CDS             complement(23710..24390)
                     /locus_tag="ACHY09_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFS3884041.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(24371..25303)
                     /locus_tag="ACHY09_001039"
     CDS             complement(24371..25303)
                     /locus_tag="ACHY09_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS3884042.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(25351..26211)
                     /locus_tag="ACHY09_001040"
     CDS             complement(25351..26211)
                     /locus_tag="ACHY09_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS3884043.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPT
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            complement(26292..27143)
                     /locus_tag="ACHY09_001041"
     CDS             complement(26292..27143)
                     /locus_tag="ACHY09_001041"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS3884044.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASVQLCVEGELGHLADAPRYDQAANADLMTQPTDVET
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            complement(27155..28246)
                     /locus_tag="ACHY09_001042"
     CDS             complement(27155..28246)
                     /locus_tag="ACHY09_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS3884045.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            complement(28271..28585)
                     /locus_tag="ACHY09_001043"
     CDS             complement(28271..28585)
                     /locus_tag="ACHY09_001043"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS3884046.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(28603..29073)
                     /locus_tag="ACHY09_001044"
     CDS             complement(28603..29073)
                     /locus_tag="ACHY09_001044"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS3884047.1"
                     /translation="MDITKILNTNRVILDMQATNKEEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(29100..30653)
                     /locus_tag="ACHY09_001045"
     CDS             complement(29100..30653)
                     /locus_tag="ACHY09_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS3884048.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENITDLLHLDHLLVAECEEIDGVMNNSRRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(30949..33267)
                     /gene="yicI"
                     /locus_tag="ACHY09_001046"
     CDS             complement(30949..33267)
                     /gene="yicI"
                     /locus_tag="ACHY09_001046"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFS3884049.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKHPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEMPAADGSVIFTLKATRTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLMVTPQGNALTITL"
     gene            complement(33277..34659)
                     /gene="yicJ"
                     /locus_tag="ACHY09_001047"
     CDS             complement(33277..34659)
                     /gene="yicJ"
                     /locus_tag="ACHY09_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFS3884050.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
BASE COUNT         8644 a         9360 c         8558 g         8246 t
ORIGIN      
        1 gcacgtactg cagaaatcaa tgaatcgcat aaaaatacaa aaaaagtata gatgttcagc
       61 aagaattggc gggtaaattg ctatttaagt caaaaacggg aaaagtaatg gtaaagccac
      121 agctaaatac atagaatccc cagcacatcc ataagtcagc tatttactat gctcgaattg
      181 ctttacaccg cccttctcta ccttattcag ccgctgatct ggatacggct ctgggtgcgc
      241 ggacgtaagg ctccggccta tcgaaaacgc tggggtgaac gttacggttt ttaccgccat
      301 ccgctaaaac caggcggcat catgctgcac tccgtctccg tcggtgaaac tctggcggcg
      361 atcccgttgg tgcgcgcgct gcgccatcgt tatcctgatt taccgattac cgttacaacc
      421 atgacgccaa ccggttcgga gcgcgtacaa tcggctttcg ggaaggatgt tcagcacgtt
      481 tatctgccat atgacctgcc cgatgcactt aatcgtttcc tgaataaagt cgaccctaaa
      541 ctagtgttga ttatggaaac cgaactatgg cctaacctga ttgcggcgct acataaacgt
      601 aaaattccgc tggtgatcgc taacgcgcga ctctctgccc gctcggccgc aggttatgcc
      661 aaactgggta aattcgtccg tcgcttgctg cgtcgtatta cgctgattgc tgcgcaaaat
      721 gaagaagatg gtgcacgttt tgtggcgctg ggcgcaaaaa ataaccaggt aaccgttacc
      781 ggtagcctga aattcgatat ttctgtaacg ccgcagttgg ctgctaaagc cgtgacgctg
      841 cgccgccagt gggcaccaca ccgcccggta tggattgcca ccagcactca cgaaggcgaa
      901 gagagtgtgg tgatcgccgc acatcaggca ttgttacagc aattcccgaa tttattgctc
      961 atcctggtcc cccgtcatcc ggaacgcttc ccggatgcga ttaaccttgt ccgccaggct
     1021 ggactaagct atatcacacg ctcttcaggg gaagtcccct ccaccagcac gcaggttgtg
     1081 gttggcgata cgatgggcga gttgatgtta ctgtatggca ttgccgatct cgcctttgtt
     1141 ggcggttcac tggttgaacg tggtgggcat aatccgctgg aagctgccgc acacgctatt
     1201 ccggtattga tggggcctca tacttttaac tttaaagaca tttgcgcgcg gctggagcag
     1261 gcaagcgggc tgattaccgt taccgatgcc acgtcgcttg caaaagaggt ttcctcttta
     1321 ctcaccgacg ccgattaccg tagtttctat ggccgtcatg ccgttgaagt actgtatcaa
     1381 aaccagggcg cgctacagcg tctgcttcaa ctgctggaac cttacctgcc accgaaaacg
     1441 cattgaggtt gttatgcaaa aacgggcgat ttatccgggt actttcgatc ccattaccaa
     1501 tggtcatatc gatatcgtga cgcgcgccac gcaaatgttc gatcacgtta ttctggcgat
     1561 cgccgccagt cccagtaaaa aaccgatgtt tactctggaa gagcgtgtgg cgctggcaca
     1621 gcaggcaacc gcgcatctgg ggaacgtgga agtggtcggg tttagtgatt tgatggcgaa
     1681 cttcgcccgt aatcaacacg ctacggtgct gattcgtggc ctgcgtgcgg tggcggattt
     1741 tgaatatgaa atgcagctgg cgcatatgaa tcgccactta atgccggaac tggaaagtgt
     1801 gtttctgatg ccgtcgaaag agtggtcgtt tatctcttca tcgttggtga aagaggtggc
     1861 gcgccatcag ggcgatgtca cccatttcct gccggagaat gtccatcagg cgctgatggc
     1921 gaagttagcg tagagtttat gccggatggt atgccatccg gcgcgcatca attacttctg
     1981 gcactgccga caataaaacg ttgcccgctg cgcatgttta gtcgccacaa tcggcgtacc
     2041 acacacccga cacgactcac cttttcgccc gtaaacctgc aattcctgag cgaaatagcc
     2101 cggtttacca tcactttgca gaaaatcttt cagcgttgta ccgccctgct caatcgaacg
     2161 cagcaacacc gctttaatca cccgagctaa caattcacac tctgccagcg acagtgatga
     2221 cgccagccga tccggatgga tccccgccgc aaacagagat tcgctggcat agatattccc
     2281 tacgcctacc accagcttgt tatccatcag ccatggttta atcgccgttt ttttcttctc
     2341 gcacttctga tgcaggtact caccgttgaa atcgtcgcta agcggctccg gtccaagatg
     2401 ggccagcacg ttgtgccctt ccagctcttt ggtccacagc caggcaccaa agcggcgcgg
     2461 atcggtgtag cgcagcactt taccgttgct catcaccaaa tcaacatggt catgcttttc
     2521 agggggaagt tcttctggaa ggatgcgcag gctgccagac atccccaaat gaatgataat
     2581 ccagccctca ggcagctcca gcagcagata tttagcccgc cgctgcacgc taagcactgg
     2641 ttggtcgctt aaacggtaga tctcttctga aaccggccag cgcaagcgtc cgttgcgcac
     2701 caccgcatga agaatggttg caccaacgag atgcggttct atgccgcgac ggctggtttc
     2761 aacttcgggt aattcaggca tagcatctcc aggaatgaac agatgcagtc aatatggggg
     2821 caggcagata acaaaaaacc ccgccggggc gaggtttttc gttacatcaa agcaaaaatt
     2881 atttgatttt cgcttctttg tagatcacgt gctggcgaac aactggatcg aattttttca
     2941 gttccagttt ttccggctta gtacgtttgt tcttcgtagt ggtatagaag tgaccagtac
     3001 cagcagaaga aaccagcttg attttctcac gaataccttt agccatgatt tatttcctct
     3061 aagtacttag tacttttcgc cacgggcacg cagttcagcc agaactgtat cgatgccttt
     3121 tttatcgatt acacgcatac ctttagcaga tacgcgcagg gtgacaaaac gcttctcgct
     3181 ctcaacccag aaacggtgag agtgcaggtt cggcaggaaa cggcgtttag tcgcgttcag
     3241 tgcgtgggaa cggttgttac cggtcaccgg acgcttgcca gtaacttggc agactcggga
     3301 catgtctatt ctccaaaaat caaattagct cgagcttcgt atggggtatt ggcgcctcgt
     3361 caggctttac agcccggtca tcgcagttct atgtgaactc tcgattgcca ggcccaaatg
     3421 ccaaacccga gattctcaaa ggtggcgtag tatacgctga ctcagcgatg tgctcaagtc
     3481 ccgaacagac aaagatcccg aaggatcgcg catagcgggt taaatccagc cgcgttcggc
     3541 aaaagaaaca tactctccac gcccaatcac gatatggtcg agcacgcgta aatccatgaa
     3601 ctgacaactc tttattatcc gttcagtaat gagtttatcc gctttactgg gttcagcaca
     3661 acccgaaggg tgattatgtg caaggatcag cgccgaggca tttattttta tcgcttcgcg
     3721 gataatttcc cgaggatgga cttcaacatg gtttagcgtg ccggaaaaaa gacggctatg
     3781 cgttataacc ctgtgttgtg agtcgagaaa gatcaccata aagatctccc gctcctcacc
     3841 cgtgagctgg ctttgtaaaa attcccgcgt catctccggg ctgagtaaag ggctttcttc
     3901 acgcatccgc acgttgtagt aacgccgcgc cagttcggca atccccttta actgggcaaa
     3961 tttcgccacg ccaattccat gaacaccact aaattgttca tattcagagg ttaacaagcc
     4021 ataaagagag ccgaaattct ccagcatctc ttttgccagg gttaatacat ctttaccgcg
     4081 cgttccggta cgcagaaata gcgccagcag ctcgacatcc gttaaggcgc taataccaaa
     4141 cttcagcatt ttttcgcgcg gcatcaacag ctgtgaattg tttttcacct tcacctcctt
     4201 tgtggtgctc gcatcctgtc atagccgttc cgttaaatcg acggccagtt ttcaatcctg
     4261 gaaagcgcct cgcaaagtga ttctcaggtg aaggcacgac aacaaaagta ttgtgataaa
     4321 atcgccaact tctggtgtca cccaacagga aaaatcatca tgagcctggc cggtaaaaaa
     4381 atcgttctcg gcgtcagcgg cggtattgct gcctataaaa cccctgaact ggtgcgtcgt
     4441 ttgcgcgagc gcggggccga cgtccgcgta gccatgaccg aagcggcaaa agcctttatc
     4501 accccactta gcttgcaggc ggtttctggt tatcccgttt ccgacagtct gctggaccca
     4561 gcagccgaag ccgctatggg ccatattgag ctgggtaaat gggctgattt agtgattctc
     4621 gcccctgcca cggcagattt gattgcccgt gttgctgccg gaatggcgaa tgatctggta
     4681 tcgacgattt gtctggccac acctgcgcct gtagccgtgc tccccgccat gaaccagcag
     4741 atgtaccgtg ccgctgccac gcagcataat ttagaggtgc ttgcttcccg tggtttgctc
     4801 atttgggggc cagacagtgg cagtcaggct tgtggtgata tcggccctgg gcgaatgctc
     4861 gatccgttaa ccattgttga tatggcggta gcgcattttt cgcccgtcaa cgacctgaaa
     4921 catctgaaca ttatgattac cgccggcccg acgcgtgaac cactcgatcc ggtgcgttat
     4981 atctctaatc acagctcagg caagatgggt tttgctatcg ccgccgccgc tgcccgtcgt
     5041 ggcgcgaacg tcacgctggt atcaggtccg gtttcactac cgacgccacc gtttgttaaa
     5101 cgtgttgatg tgatgaccgc gctggaaatg gaagccgccg tgaatgcatc tgtacagcag
     5161 caaaatattt ttatcggttg cgccgccgtg gcggattatc gcgcagctac cgtggcccca
     5221 gaaaaaatca aaaagcaggc cacgcagggt gatgaattaa caataaaaat ggttaaaaac
     5281 cccgatatcg tcgcaggcgt tgccgcacta aaagaccatc gcccctacgt cgttggattt
     5341 gccgccgaaa caaataatgt ggaagaatac gcccggcaaa aacgtatccg taaaaacctt
     5401 gatctgatct gcgcgaacga tgtttcccag ccaactcaag gatttaacag cgacaacaac
     5461 gcattacacc ttttctggca ggacggagat aaagtcttac cgcttgagcg caaagagctc
     5521 cttggccaat tattactcga cgagatcgtg acccgttatg atgaaaaaaa tcgacgttaa
     5581 gattctggac ccgcgcgttg ggaaggaatt tccgctcccg acttatgcca cctctggctc
     5641 tgccggactt gacctgcgtg cctgtctcga cgacgccgta gaactggctc cgggtgacac
     5701 tacgctggtt ccgaccgggc tggcgattca tattgccgat ccttcactgg cggcaatgat
     5761 gctaccgcgc tccggattgg gacataagca cggtatcgtg cttggtaacc tggtaggatt
     5821 gatcgattct gactatcagg gccagttgat gatttccgtg tggaaccgtg gtcaggacag
     5881 cttcaccatt caacctggcg aacgcatcgc ccagatgatt tttgttccgg tagtacaggc
     5941 tgaatttaat ctggtggaag atttcgacgc caccgaccgc ggtgaaggcg gctttggtca
     6001 ctctggtcgt cagtaacaca tacgcatccg aataacgtca taacatagcc gcaaacattt
     6061 cgtttgcggt catagcgtgg gtgccgcctg gcaagtgctt attttcaggg gtattttgta
     6121 acatggcaga aaaacaaact gcgaaaagga accgtcgcga ggaaatactt cagtctctgg
     6181 cgctgatgct ggaatccagc gatggaagcc aacgtatcac gacggcaaaa ctggccgcct
     6241 ctgtcggcgt ttccgaagcg gcactgtatc gccacttccc cagtaagacc cgcatgttcg
     6301 atagcctgat tgagtttatc gaagatagcc tgattactcg catcaatctg attctgaaag
     6361 atgagaagga caccacagcg cgcctgcgtc tgattgtgtt gctgcttctc ggttttggtg
     6421 agcgtaatcc tggcctgact cgcatcctca ctggtcatgc gctaatgttc gaacaggatc
     6481 gtctgcaagg gcgcatcaac cagctgttcg agcgtattga agcgcagttg cgtcaggtat
     6541 tgcgtgaaaa gagaatgcgt gagggtgaag gttacaccac cgatgaaacc ctgctggcaa
     6601 gccagttgct ggccttctgt gaaggtatgc tgtcacgctt tgtccgcagc gagtttaaat
     6661 accgcccgac ggatgatttt gacgcccgct ggccgctaat tgccgcacag ttgcagtaat
     6721 atgacgccgg atgacttttc atccggcgag tttctttaaa cgccaaactc ttcgcgatag
     6781 gccttaaccg ccgccagatg ttccgccatt tctggcttct cttccaggta agcaatcagg
     6841 tctttcaggg tgatgataga gatcaccttg cagttgtaat cacgctcaac ttcctgaatc
     6901 gccgaaatct cgccgcgccc gcgttcctga cgatcaagcg aaatcaatac gccagccagc
     6961 gtcgcgccat tggcctgaat aatttccatc gactcgcgaa tcgccgttcc ggcggtgatc
     7021 acatcatcca ccagcattac gcgtccttgt aacgcgctac cgaccagatt gccaccttca
     7081 ccatggtctt ttgcttcttt gcggttaaag cagtacggca ggtccaggtc atgatgctcc
     7141 gccagcgcca cggcagttgt ggtggcaatc gggatccctt tgtaagcagg gccaaacagc
     7201 agatcgaact caatgccgga atccaccaat gcttcagcgt aaaaacggcc taacagtgcc
     7261 agatcgcgcc cggtattaaa cagcccggcg ttgaagaaat aggggctttt gcgcccggat
     7321 ttcagcgtaa actcgccaaa ctttaacacc tgcttgctaa gcgcaaattc aataaactgg
     7381 cgctgatatg gtttcatgcc ttcgctcctc atcttacttt tctacagaca aaaaaaaggc
     7441 gactcatcag tcgccttaaa aatcagtttg ccagcgccgc cttctgcgtc gctacaatgg
     7501 attcgattcc ccctcgggcc agcgccaaca aggtgagtaa ctcttcgtgg gtgaacggtt
     7561 cgccttctgc cgtcccctgc acttcaatga tgcgcccgtc ttcggtcatc actacgttca
     7621 tgtcggtctc tgcggctgag tcttcaacgt attccagatc gcaaaccgct tcgccgttca
     7681 caattccgac agaaactgcg gctaccatcc ctttcatcgg gttggttttc agctttccgt
     7741 tttccaccag cttctgtagc gcatctgcca gcgccacgca ggcacccgta atcgacgcgg
     7801 tacgagtgcc accatcagcc tgaagcacgt cgcagtccag cgtaatggtg aactcaccca
     7861 gcgctttcaa atctactgcc gcacgaagag cacgggcgat cagacgctgg atttccatgg
     7921 tgcgtccacc ctgcttacct ttcgccgctt cacgcgcgtt acgggtgtgg gtagaacgtg
     7981 gcagcatgcc gtactctgcg gtgatccagc cctggccctg acctttcagg aagcgcggca
     8041 cgccttcttc aatagaggcg gtacacaata ctttggtatc gccaaattcg accagcaccg
     8101 agccttctgc atgttttgta tagttacgag tcagggtaac gggacgcacc tgattattgc
     8161 tacggcctgc tggacgcata ttgaaatctc cggcttgaaa caaatgtggc tgcgcattat
     8221 acggacttcc ggcggttatt cctatcctga caaggcatcg atggctataa tccttccacc
     8281 tctcctttta taaacaggaa cgtctatgat ccgcagtatg accgcctacg cccggcgtga
     8341 aatcaagggt gaatggggga gcgcaacctg ggaaatgcgc tcggtaaacc agcgttatct
     8401 ggaaacttac tttcgtctgc cggagcagtt ccgtagcctt gaacctgtcg ttcgtgagcg
     8461 tattcgttct cgcctgacgc gcggtaaagt ggaatgtacc ctgcgctatg agccagatgt
     8521 tagcgcgcaa ggtgagctga tcctcaacga aaaactggct aaacagctgg taactgccgc
     8581 gaactgggta aaaatgcaga gtgacgaagg ggaaatcaac ccggttgata ttctacgctg
     8641 gccgggcgtg atggcagccc aggagcagga tctcgacgcg attgccgctg aaattctcgc
     8701 ggcgttggat ggtacgctgg acgactttat cgtcgcgcgc gaaaccgaag gtcaggcgct
     8761 gaaagcattg atcgagcagc gtctggaagg cgtcaccgcc gaagtggtca aagtccgcgc
     8821 ccatatgccg gaaatcctgc aatggcagcg tgagcgtctg gtcgcgaagc tggaagatgc
     8881 tcaggtgcaa ctggaaaaca accgtctgga gcaggagttg gttctgctgg cacaacgaat
     8941 tgacgttgcc gaagaactgg accgcctcga agcgcatgtc aaagagacct acaacattct
     9001 gaagaaaaaa gaagcggttg gtcgtcgtct ggactttatg atgcaggagt tcaaccgcga
     9061 gtcgaacact cttgcgtcga aatctatcaa tgccgaagtg acgaattctg ccatcgagct
     9121 gaaagtgttg attgagcaga tgcgcgagca gatccagaac attgaataac tcgtaacgcc
     9181 aattcttcct tttcagcctt cacaaatgcc gcccacttaa acagagcggc atttttcctc
     9241 cccgcaacat tcaattctgt tttgcgtgcc tgctccagat tttgcgatgt ttttttgccc
     9301 agcgcagtga gaacgtgaga tacttacaac tgtatataaa cacagttgta gacttacttt
     9361 ccttgcaatc gatatcacat ggagtgggca atgaacgaac atcatcaacc ttttgaagag
     9421 ataaagctga ttaatgcaaa cggagcagaa caatggtcag caaggcaact tggaaaacta
     9481 ctgggttatt cagagtatcg tcactttata cctgtattaa cgcgcgccaa agaagcttgt
     9541 ctgaatagtg gtcacacaat tgatgaccat ttcgaggaga tcctcgatat ggtcaaaatt
     9601 ggctcaaatg ccaaaagagc attaaaagac atcgtactct cccgctatgc ctgttacctg
     9661 gtagtacaaa acggcgaccc tgcgaaaccg gttattgcgg cagggcaaac ttattttgct
     9721 atccagaccc gacggcagga gctggctgac gatgaagctt tcaggcaact tcgtgaagac
     9781 gaaaagcgtc tttttctgag aaatgagttg aaagaacata acaaacaatt agttgaggca
     9841 gcgcagcaag ctggggtagc gacagctatc gatttcgcca tatttcagaa tcatggttac
     9901 cgggggctgt atggtggatt agatcagaaa gccatccatc agcggaaggg gctgaaaaag
     9961 agtcagaaga tcctggatca tatgggttca acagaactgg cggctaatct ctttcgagct
    10021 acccaaacag aagaaaaact caagcgggat ggcgtgaatt caaaacagca agctaacacc
    10081 acacactttg acgtgggtcg caaggtgagg caaaccattc aggaacttgg cggaaccatg
    10141 cctgaggagt tgccgacccc gcaggtcaac atcaagcagt tggaaaacag tgtaaaaatt
    10201 acagagaaga aatagcagaa actaaaaact ctttttgttg attgagatac ccgatgcgta
    10261 agccaaagtc caggtgcggt gaacacaatg gctaaacatt gcacctttct tttccacccc
    10321 tcaattttaa cctatttttt cttatgcatt ttctcagaca agaagtcaga agaatgcatc
    10381 tacgctacag aaaatagcga tttcacataa ctacaagtta tcaatttcct ctcaccttaa
    10441 aaaatctcaa tcgtgacaat acgcacaaat cgctaccctg ccagacagat ttttagggag
    10501 agaaccatgc tgttacacat tttgtatttg gttggcatta ctgccgaagc catgacgggg
    10561 gcgctggcgg ccggacgacg gcgcatggat acatttggcg taattattat tgcgaccgcc
    10621 acggcaattg gcggagggtc agtgcgcgat attctgctgg gccactatcc gctcggctgg
    10681 gtcaaacacc cggaatatgt gattattgtc gctaccgccg cagtactcac cactatcgtt
    10741 gctcctgtaa tgccttacct gcgcaaagtg tttctggtgc tcgatgcgct cggactggtg
    10801 gtcttttcta tcattggcgc acaggttgca ctggatatgg gacacggccc gatcattgcc
    10861 gttgtcgcag ctgtgacgac cggcgtattt ggcggcgttt tgcgcgatat gttctgtaaa
    10921 cgcatcccgc tggtattcca gaaagagttg tatgccggtg tctcatttgc ctccgccgtg
    10981 ctgtacatcg cgctgcaaca ctatgtttct aaccatgatg tggtaattat ctccaccctg
    11041 gtattcggct tttttgcccg cttactggcg ctacgcctta agctgggatt accggtcttt
    11101 tactacagcc acgaaggaca ctaaggttca aaacctgtga tctgctgggc agccagccaa
    11161 ctgcccagct tcttgatttg cgcattttcc ttccattcaa taacctgtct gacacgcccc
    11221 gatccagtcc ccggcagctg ctgccagaac tgctccgtgc tcaataaaag ttgcgaccag
    11281 gaccgttcat cactggcatt aagcgccgcc cgggttagcg gtattcccat tgccatcacc
    11341 cagcgagtaa aaggctgctt acgagccaga ttaaactgat gccatagctg cgcactttta
    11401 cttttcgcga tccccggcgt gttctgtaat tgctctggcg ttaataaaag ccaggaaaag
    11461 atatgctcaa agcgatgagt ctgatgcagc gagcgccagc cggcctcacc aatgccatcc
    11521 agcccaagaa cctgttttga ccccagccag actaagcgtg aaatgaactg ttcctgacaa
    11581 acatcagaag caaagtagca ggtcaacgag ttaaagcggt tttctggcgg tgtcggtttt
    11641 gtacgttctg caccgcgcca caccacatca tcaatgcgag gaataccctg accggcaagg
    11701 ctgacgagga tctgatcacc aggcgcaata tcccactcct gccagcgcct gacggaacca
    11761 atattcaccc gctggacttt tttatcatca agcatgacag gtgcgagtga cgcaaccacc
    11821 gatattttac cgctcttacc caccgcaaac tgaatcgcct tcacttcggc aacccgagct
    11881 acaggttgat atttccaggc caccagccat tctgcctggc ctggtagcca atggcgggat
    11941 tcgggttctt tcgccgctcg cacaaccacg ccatcggtga cgaagggtaa tttcgttttc
    12001 caccacgcat tgcgtacgcg cgcaacttca tcagcatttt tcaccgcacg ggtatacgtc
    12061 tgcgttagag taaaaccagc ggtagccagc tcttttaaac gatcggtcat taactgcggt
    12121 ccatccggcc atgcccagac aaaaacgccc agagaattca gcgtgtcgct atcgtcctgg
    12181 cgcatcatca gaccagcaac ttttgtgcgg gcatttattc cccccatttg tttttggata
    12241 tgcccctcgc gtttgagaaa tatttccccc tgaagcgtac tgttggctaa aggcccgcta
    12301 acggtttgcg gcacagcgga aattaagcga actttctgcg tccagtcctc gcctttcagg
    12361 ccattgccgc gactgattgc tttgttcagt ttcccgtccc gataaaccag ggttaccgca
    12421 acgccatcaa cttttggctg cacccaaaga tcgctacgtt cgcgcatcca cagacttaat
    12481 gcgattttat ccgccatttt gcgtacaccc gtatgtgcaa ccggatgtat aaccgcaccg
    12541 tttaaaggcg gcatcatagc gtcgcgatgc gtctcttcac caaaacagcg ttgccactgc
    12601 gtgagacggg cacttaactg atcgtaaata ccgtcttcca cctcactttt cccttctttc
    12661 cagtagtcat cgtcccactg ttttatttgc tgttgcaggc gggaaatttc ttcctgtgct
    12721 ctggctggcg accaggccgg acagactgcc cacgccgatg attgccagca caagatactt
    12781 attaatatcg ccatccatac tttcatcatc acctccgctg tagatagtca ggcagatata
    12841 caacgtgatg aaagcaaagc cgagtggcaa aaacggagtc tgcgaggacg cttcctgaga
    12901 atcgtcttta ttgcagtgaa tgacaggtaa atgcggaagc agctacgcaa aacgcaacaa
    12961 ctttgcgcaa aaagtgtgag caagggctac gtcacatggc cgcgccgtgt ataataagct
    13021 cgtatgtagg ctttatttcg ctaatcacat acgaaagata ctcatggctc aaggcacgct
    13081 ttatattgtt tctgccccca gtggcgcggg taaatccagc ctgattcagg ctttattaaa
    13141 aacccaaccg ttgtatgaca cccaggtttc tgtttcacac accacacgcc aaccgcgtcc
    13201 tggtgaggtc cacggtgaac attatttctt tgttaatcat gatgaattta aagaaatgat
    13261 tagcagagat gcgttcctcg aacacgcaga agtttttggt aattactatg gcacttcgcg
    13321 tgaggccatt gagcaagtac tggcgaccgg tgtcgatgtt tttctcgata tcgactggca
    13381 gggtgcgcag caaattcgcc agaagatgcc gcacgcgcgg agtatcttta ttttaccgcc
    13441 gtccaaaatt gaactggacc gccgtctacg cggtcgcggt caggacagcg aagaggtcat
    13501 tgcaaagcgt atggcgcaag ctgttgcaga aatgagccat tacgccgaat atgattacct
    13561 gattgtgaat gatgacttcg ataccgcgtt gaccgatttg aagaccatta ttcgcgccga
    13621 acgtctgcgc atgagccgcc aaaagcagcg tcatgacgct ttaatcagca aattgttggc
    13681 agactgaacc tgatttcagt atcatgccca gtcatttctt cacctgtgga gctttttaag
    13741 tatggcacgc gtaactgttc aggacgctgt agagaaaatt ggtaaccgtt ttgacctggt
    13801 actggtcgcc gcgcgtcgcg ctcgtcagat gcaggtaggc ggaaaggatc cgctggtacc
    13861 ggaagaaaac gataaaacca ctgtaatcgc gctgcgcgaa atcgaagaag gtctgatcaa
    13921 caaccagatc ctcgacgttc gcgaacgcca ggaacagcaa gagcaggaag ccgctgaatt
    13981 acaagccgtt accgctattg ctgaaggtcg tcgttaatca caaagcgggt cgcccttgta
    14041 tctgtttgaa agcctgaatc aactgattca aacctacctg ccggaagacc aaatcaagcg
    14101 tctgcggcag gcgtatctcg ttgcacgtga tgctcacgag gggcaaacac gttcaagcgg
    14161 tgaaccctat atcacgcacc cggtggcggt tgcctgcatt ctggccgaga tgaaactcga
    14221 ctatgaaacg cttatggcgg cgctgctgca tgacgtgatt gaagatactc ccgccaccta
    14281 ccaggacatg gaacagcttt ttggtaaaag cgtcgccgag ctggtagagg gggtgtcgaa
    14341 acttgataag ctcaagttcc gcgataagaa agaggcgcag gccgaaaact ttcgcaagat
    14401 gattatggcg atggtgcagg atatccgcgt cattctcatc aaacttgccg accgtaccca
    14461 caacatgcgc acgctgggct cacttcgccc ggacaaacgt cgccgcatcg cccgtgaaac
    14521 cctcgaaatt tacagcccgc tggcgcaccg tttaggtatc caccacatta aaaccgaact
    14581 cgaagagctg ggttttgagg cgctgtatcc caaccgttat cgcgtaatca aagaagtggt
    14641 gaaagccgcg cgcggcaacc gtaaagagat gatccagaag attctttctg aaatcgaagg
    14701 gcgtttgcag gaagcgggaa taccgtgccg cgtcagtggt cgcgaaaagc atctttattc
    14761 gatttactgc aaaatggtgc tcaaagagca gcgttttcac tcaatcatgg acatctacgc
    14821 tttccgcgtg atcgtcaatg attctgacac ctgttatcgc gtgctgggcc agatgcacag
    14881 cctgtacaag ccgcgtccgg gccgcgtgaa agactatatc gccattccaa aagcgaacgg
    14941 ctatcagtct ttgcacacct cgatgattgg cccgcacggc gtgccggttg aggtccagat
    15001 ccgtaccgaa gatatggatc agatggcgga gatgggtgtt gccgcgcact gggcttataa
    15061 agagcacggc gaaaccagca ctaccgcgca aatacgcgcc cagcgctgga tgcaaagcct
    15121 gctggagctg caacagagtg ccggtagctc gtttgaattt atcgagagcg ttaaatccga
    15181 tctcttcccg gatgagattt acgttttcac accagaaggg cgcattgtcg aactgcctgc
    15241 cggtgcaacg cccgtcgact tcgcttatgc ggtgcatacc gatatcggtc atgcctgcgt
    15301 gggcgcacgc gtcgaccgcc agccttaccc gctgtcgcag ccgcttacca gcggtcaaac
    15361 cgttgaaatt attaccgcac cgggcgctcg cccgaatgcc gcgtggctga actttgtcgt
    15421 tagctcgaaa gcgcgcgcca aaattcgtca attgctgaaa aacctcaagc gtgatgattc
    15481 tgtaagcctg ggccgtcgtc tgctcaacca tgcgttgggt ggcagccgca agctcaatga
    15541 aatcccgcag gaaaatattc agcgcgagct ggaccgcatg aagctggcaa cgcttgacga
    15601 tctgctggct gaaatcggcc ttggtaacgc aatgagcgtg gtggtcgcga aaaatctgca
    15661 acatggggac gcctccattc caccggcaac ccaaagtcac ggacatctgc ccattaaagg
    15721 tgccgatggc gtgctgatca cctttgcgaa atgctgccgc cctattcctg gcgacccgat
    15781 tatcgcccac gtcagccccg gtaaaggtct ggtgatccac catgaatcct gccgtaacat
    15841 ccgtggctac cagaaagagc cagagaagtt tatggctgtg gaatgggata aagagacggc
    15901 gcaggagttc atcaccgaaa tcaaggtgga gatgttcaat catcagggtg cgctggcaaa
    15961 cctgacagcg gcaattaaca ccaccacctc gaatattcaa agtttgaata cggaagagaa
    16021 agatggtcgc gtctacagcg cctttattcg tctgaccgcc cgtgaccgtg tgcatctggc
    16081 gaatatcatg cgcaaaatcc gcgtgatgcc agacgtgatt aaagtcaccc gaaaccgaaa
    16141 ttaatgtttt atgaacccaa cacgttatgc acgcatctgc gaaatgctcg ccaggcggca
    16201 gcctgatctg accgtctgca tggagcaggt ccacaaacct cataacgttt ctgcgattat
    16261 tcgtaccgca gatgccgttg gtgtacatga agttcatgct gtctggcctg gtagccggat
    16321 gcgcaccatg gcttcggcag ctgcgggtag taacagctgg gtacaggtga aaacgcaccg
    16381 caccattggc gatgccgtcg ctcatctgaa aggccagggc atgcagattc tggcaaccca
    16441 tctttctgat aacgctgtcg atttccgcga aattgattac actcgcccga cctgtatttt
    16501 gatgggacag gagaaaacag gcatcacgca ggaagcgttg gccctggcgg atcgggacat
    16561 catcataccg atgatcggca tggtgcagtc gctgaatgtt tccgttgcct cagccctcat
    16621 tctttatgaa gcccagcgcc agcggcaaaa tgcaggcatg tacctgcgtg aaaacagcat
    16681 gttgccggaa gcagagcaac aacgcctgct gtttgaaggc ggctatccgg tgctggcgaa
    16741 agtcgcaaaa cgcaaaggcc tgccttaccc ccacgttaat cagcaaggcg aaattgaagc
    16801 cgatgctgac tggtgggcta ccatgcaggc tgcagggtaa gtgccatgaa aggtcgcctg
    16861 ttagatgctg tcccgctcag ttccctaacg ggcgttggcg cagcgcttag taacaagctg
    16921 gcgaaaatca acctgcatac cgtgcaggat ctactcttac accttcccct gcgctacgaa
    16981 gatcgcaccc atctctaccc catcggagaa ctactaccgg gcgtttatgc cacggtggaa
    17041 ggcgaagtgc tgaactgcaa tatctccttc ggcggtcggc ggatgatgac ctgccagatc
    17101 agcgacggtt ccggcattct caccatgcgc tttttcaact tcaacgcggc aatgaaaaat
    17161 agcctggcga caggccgccg cgtgctggcc tatggcgaag caaagcgcgg taaatatggt
    17221 gcggagatga tccacccgga ataccgcgtg cagggcgatc tcagtacgcc agagttgcag
    17281 gaaacgctaa cgccggttta cccaacaacg gaaggcgtaa agcaggccac gctgcgtaaa
    17341 ttaaccgatc aggcgctgga tctgctcgac acctgcgcca ttgaagaact cctgccgccg
    17401 gaactgtcac aaggaatgat gacgctaccg gaagcgttgc gcactttgca ccgcccgccg
    17461 ccgacgctac agcttagcga tctcgaaacc gggcagcatc cagcacaacg ccgtctgatt
    17521 ctggaagaac tgcttgcgca caacctcagc atgttggcgt tacgcgctgg tgcacaacgt
    17581 ttccatgccc agccgcttag cgccaatgac gcgctgaaaa ataaactcct cgcagcctta
    17641 ccgttcaagc caacgggcgc acaggcacgc gtggtggcgg agatcgagcg cgatatggcg
    17701 ctggatgcgc cgatgatgcg cctggtacag ggcgatgtag gttccggtaa aacgctggtc
    17761 gctgccctcg ccgcgctgcg tgcgattgcg cacggcaaac aggtggcatt gatggcacca
    17821 accgaattac tcgccgaaca gcacgcgaat aacttccgca actggtttgc accgctcggt
    17881 atcgaagtgg gctggctcgc cggtaagcag aaaggtaaag cgcgactggc acagcaggaa
    17941 gccatcgcca gcggtcaggt gcagatgatt gtcggaacac acgccatctt ccaggaacag
    18001 gtgcagttta acggcctggc gctggtgatt atcgacgaac agcatcgttt tggcgtgcat
    18061 cagcgtctgg cgttatggga gaaaggccag cagcaaggct tccatcctca tcagttgatc
    18121 atgaccgcca cgccgatccc ccgaacgctg gcaatgactg cgtatgctga tctcgatacc
    18181 tcggtgatag atgagctgcc gccaggccgc acgccagtga ctacggtcgc tattcctgat
    18241 acccgccgta ccgacattat tgaccgcgtg cgccacgcct gcataactga aggtcgtcag
    18301 gcatactggg tttgtacgtt gattgaagag tcagagttac tggaagcgca ggcggcggaa
    18361 gctacctggg aagagttgaa actggcgcta ccagagttga atgttggtct ggtacacgga
    18421 cggatgaaac cagccgagaa gcaggccgtc atggcatcat ttaaacaagg cgagttacac
    18481 ctgctggttg ccactaccgt tattgaagtc ggcgttgatg tgcctaacgc cagcctgatg
    18541 attatcgaaa acccggagcg tctgggtctg gcgcagttgc accagcttcg cgggcgcgtg
    18601 ggacgtggtg cggtggcttc tcactgcgtg ctgctctaca aaacgccgct ctctaaaacg
    18661 gcgcaaattc gtctgcaagt actgcgcgac agcaacgacg ggtttgtgat tgcgcaaaaa
    18721 gatctggaga ttcgcggccc tggcgaattg ttaggcacgc gtcagacggg taatgctgaa
    18781 tttaaagtgg cggatttact gcgcgatcag gcgatgatcc cggaagttca gcgcctggca
    18841 cgccatattc acgaacgtta cccacagcag gcaaaagccc tgatagaacg ctggatgcca
    18901 gaaacggaac gttactcgaa tgcgtaaaag gcggcggtga gaagaccgcc gcttcatcgg
    18961 ttaaccggca aaaatcggca acattaaata cagcttaatc accagcgcgt taacgatatc
    19021 aataaagaac gcaccgacca tcggcactac caggaaggcc atgtgcgacg ggccaaaacg
    19081 ttcagtgatc gcctgcatgt tggcgattgc tgttggcgtt gcaccgaggc caaaaccaca
    19141 gtgacccgca gccagcactg ccgcatcgta gtttttgccc atcatgcgcc aggtaacgaa
    19201 gatggcatac aacgccatga agatggtctg taccaccaga atcgccagca tcggcagcgc
    19261 cagcgaagcc agctcccata gtttcagccc catcagcgcc atcgccagga acaacgacag
    19321 gcttacgtta cccagcacgg ataccgcacg ctcaaagacg cggtaaaagc ccatcattga
    19381 cagaccgttg ctcagaatca cgccaacaaa cagtacacag acgaaggtcg gcagttcaaa
    19441 agcagtgcca gccaaaagtt gcgcaacaat tttccccacc gtcaggcaga tagcaatcag
    19501 cgcgatagtt tcaatcagca ccagcgaggt gatcatgcgg cccacatccg gcttttcgaa
    19561 cgccgtcggg acttcctggt catccggaat accgttcggc gtggtggagt gtttcaccag
    19621 atagcgcgct accggaccgc caatcaagcc gcccagcacc agaccaaacg tcgcacaggc
    19681 catcgccact tccgtcgcat tggtgaagcc atagcgttca atgaacaatt tactccacgc
    19741 agcgcccgta ccgtgaccgc cggaaagcgt aatagaaccg gccaacagcc ccatcagcgg
    19801 atcaagccct aacaggctag ccataccaat gccaatggca ttttgcatca ccaacagacc
    19861 aacaaccaca atcaagaaga tgccaaccac acgcccaccg gcacgcaaac tggcaatgtt
    19921 ggcgttcagg ccaatggtgg cgaagaatgc cagcattaac ggatcgcgca gggacatatc
    19981 aaagttgact tcccagccca tgcttttttt cagtaccagt agcgccagcg ccaccaacaa
    20041 accacccgca acaggttccg gtatggtgta tttcttcaaa aaggagacgg aatggaccaa
    20101 cttacgcccg agcagcaacg tcagcgttgc ggcaacaagc gttgctaaag tatcgagatg
    20161 aaacatagtt actcctttgt atccgcatca ctctttcata ctcgtcatac ttcatcgcgc
    20221 agtggcaact tatgaactat gacgggtatc gagttatgct ttcctgggtc aaattctcag
    20281 ctgttaatga acaaatggca tggattttaa gcagaaaaat accaaaagtt atataaaaaa
    20341 gtgcatttat ctcatttaaa tatttagcag aggccgtggc aaacgtttgc ttttctcttt
    20401 cggtcagcta aaatgcccgc tttgctacaa cgggattgtt ttcgatgtct gtttccaccc
    20461 tcgagtcaga aaatgcgcaa ccggttgcgc agactcaaaa cagcgaactg atttaccgtc
    20521 ttgaagatcg tccgccgctt cctcaaactc tgtttgccgc ctgtcagcat ctgctggcga
    20581 tgttcgttgc ggtgatcacg ccagcgctat taatctgcca ggcgctgggt ttaccggcac
    20641 aagacacgca acacattatt agtatgtcgc tgtttgcctc cggtgtggca tcgattattc
    20701 aaattaaggc ctggggtccg gttggctccg ggctgttgtc tattcagggc accagcttca
    20761 actttgttgc cccactgatt atgggcggta ccgcgctgaa aaccggtggt gctgatgttc
    20821 ctaccatgat ggcggctttg ttcggcacgt tgatgctggc aagttgcact gagatggtga
    20881 tctcccgcgt tctgcatctg gcgcgccgca ttattacgcc gctagtttct ggcgttgtgg
    20941 tgatgattat cggcctgtcg ctaattcagg ttgggttaac gtccattggc ggcggttacg
    21001 cagccatgag cgataacacc ttcggcgcac cgaaaaatct gctgctggca ggcgtggttt
    21061 tagccttaat tatcctgctt aaccgtcaac gtaaccctta cttacgcgtg gcctcactgg
    21121 taattgcgat ggcggccgga tatgcgctgg cgtggtttat gggcatgttg ccagaaagca
    21181 acgaaccgat gacacaagaa ctgattatgg tgccaacgcc gctctattac ggtcttggca
    21241 ttgaatggag tctgctgctg ccgctgatgc tggtctttat gatcacttcg ctggaaacca
    21301 ttggcgatat cacggcgacc tctgacgttt ccgaacagcc agtgtccggt ccgctgtaca
    21361 tgaaacgcct gaaaggcggt gtgctggcaa acggcctgaa ctcgtttgtt tcggcggtgt
    21421 ttaacacctt cccgaactcc tgcttcgggc agaacaacgg agtgatccag ttaaccggtg
    21481 ttgccagccg ctatgtcggt tttgtcgtcg cgctgatgtt gatcgtgctg ggtctgttcc
    21541 cggcagtgag cggttttgta caacacattc ctgaaccggt tctgggcggc gcaacgcttg
    21601 taatgtttgg caccatcgcc gcctccggtg tgcgtatcgt ttctcgtgag ccgctgaacc
    21661 gtcgggcgat tctgattatc gcgctgtcgc tggcggtcgg tctgggcgtc tctcagcagc
    21721 cgctgatttt gcagtttgcc cctgaatggc tgaaaaacct gctctcctcc gggattgccg
    21781 cgggcggtat tactgccatc gtgctgaatc tgattttccc accagaaaaa cagtaattca
    21841 ctggcggcgg taagataatt accgccgcag tctcacgtct ttcaccattc ccgccttgag
    21901 gattgcgcgt aaatcgtgca taactccctt atgtgctttt cacgggatgg aagaccatga
    21961 aatttattgg gaagctgctt ctctacattc tcatcgctct gttagtggtg atcgctggcc
    22021 tctattttct tctgcaaacc cgctggggag cagaacatat cagcgcatgg gtttccgaga
    22081 atagcgacta tcatctggcc ttcggggcga tggatcaccg tttttccgcg ccatctcata
    22141 tcgtgctgga gaacgtcacg tttggtcgtg atggtcagcc cgcgaccctg gtggccaaaa
    22201 gtgtcgacat tgcgctaagc agtcgccaac tgaccgaacc acgccatgtc gataccatct
    22261 tgctggaaaa cgggacgctg aatctcaccg accagaccgc gccgctaccg ttcaaagccg
    22321 atcgtctgca actgcgtgat atggcgttta atagcccgaa tagcgaatgg aaactaagcg
    22381 cgcagcgggt aaatggtggc gtggttccgt ggtcaccaga agccggtaaa gtgctgggta
    22441 cgaaggcgca gattcagttt agtgccggat cgctttcgct caatgatgtt cctgccacca
    22501 atgtactgat tgaaggcagt attgataacg atcgcgttac gctgactaac ttgggtgccg
    22561 acatcgcccg cgggacatta accggaaacg cgcagcgtaa cgccgacggc agctggcaag
    22621 tggaaaatct gcgcatggcg gatatccgtc tacaaagcga aaaatcgcta accgacttct
    22681 ttgcgccatt acgctctgtc ccgtcgttgc agattggtcg cctggaagtc attgacgctc
    22741 gtttgcaagg tccggactgg gcggtgaccg acctcgatct cagcttgcgc aacatgacct
    22801 tcagtaaaga tgactggcag acacaggaag gcaaactgtc gatgaacgct agcgagttca
    22861 tttatggttc gctgcattta tttgacccga ttataaacgc ggaattttcc ccgcagggcg
    22921 tagcgctgcg ccagttcacc agccgctggg aagggggtat ggtcagaacg tcagggagct
    22981 ggttgcgtga cgggaaaacg ttactccttg atgatgcggc aattgccggg ctggaatata
    23041 ccttgccaaa aaactggcaa cagttgtgga tggaaacgac acccggttgg ttaaatagcc
    23101 tgcaactgaa gagatttagc gccagccgca atctgatcat tgatatcgac cctgacttcc
    23161 cgtggcagct caccgcgctc gatggttacg gtgccaacct gacgctggtt accgatcata
    23221 aatggggcgt ctggagtggt tcggcgaatc tgaatgccgc cgccgcgaca ttcaatcgtg
    23281 ttgatgttcg tcgcccatcg ctggcgctga ccgccaacag cagcacggtg aatatcagcg
    23341 aactgagtgc atttactgaa aaaggcattc tggaagccac cgccagtgtt tcacaaacgc
    23401 cacaacgtca gacccatatc agcctgaatg gacgcggtgt gccggtgaat attttgcaac
    23461 agtggggatg gcctgaatta ccgttgactg gcgacggcaa tattcagctt actgccagtg
    23521 gcgatattca ggccaatgtc ccgctgaaac ctacggttac cgggcaattg catgccgtga
    23581 atgccgcaaa gcagcaagtg actcaaacca tgaatgcggg cgtagtttcc agtggtgaag
    23641 ttacgtcgac ggagccggtg cagtaaaaat aaggacgcta gcggatatca gcgctagcgt
    23701 tccccgttat tagcgcaaaa ttccatactc actgcacaat aaccatttca ccggttcatc
    23761 ttcttctatc gccggaaaac gtcctcccgg ctcaagaaaa taattatcgt gttggtcatc
    23821 atttaccatc gagatttccc agccgagaac aaacgctttt tccgcccaga agcgatgata
    23881 cagacgcggc ggcaggcaaa tgccctgccc cggctttagc accagcgttt caccaggttt
    23941 catggtacgc cgctgaccat caacactcac ggtaatgact cgttgatcat cacagagatt
    24001 ggcttcgttg gcccaggcca actgcatgca taagtctcca ccgccccgat tgaggatatc
    24061 ttccattttg tgggtgtgaa aatgccaggg agtctgctga tcttgttgga tctgcaacat
    24121 tttctcagca tacggttttg gatatgccgc cgaccccagc gcgccgttgc gcatcgttag
    24181 cagtgttagt cccgtttggt tgaaatcacc acgaccaaaa tcggtgatat cccaaccgag
    24241 ctgtaagtcg accacttctt gccagttatc ccgttcccgc tgcagccagt catccaccgt
    24301 aaaataggcg aactctggca aacaaacgcg gaaggtctgc gcaatggcat gtgctttatc
    24361 aataacataa ttaatttgtg aacgtttcat tggcttctcc gttgcgcata gcaagccagg
    24421 cagcgccacg cattccgcta ctgtcgccgt gctgtgcttt tacaatggga gtggtgaatt
    24481 gatcggtgaa aaccagtgga gcgacttctg cgttcagatc ggtgataagc agttccacat
    24541 tcgaaagccc gccgccgatc acaatcacgc cagggtcaac aacattaacg atggttgcca
    24601 gcgtgcgggc aagctgttgg cgaaaacgtg ccacctgctg catggcacaa gcatcaccac
    24661 gttgtgccag ggtgacaatc gccgcaggag tgagcaagtt accagtcatc tgttgatagc
    24721 gttcactaaa accgctgccg gaaacaaacg actccacgca gttgtgtttg ccgcaatagc
    24781 aactgactga tggaccatct tcctgctccg tatagcctgg caacgtgata tgaccgcatt
    24841 cggcagcatt tccccaggcc cctataaacg gtcgttgctt gatggcaatg ccaccgccgc
    24901 agcccgtacc aagcgtaata ccaaatacca cgtcataatc ttgcccggca ccgtcgcaag
    24961 cttctgatag cgcgaaacag ttaccatcat tggctatcac caccggttgc ccaagcaatt
    25021 gctgcaaatc agcctgtaac gcatgaccgt taatcacctg aatattcgcg tttttaatca
    25081 ggccagtgag tggcgagata ctgccgggta aggcgatccc tgtcagcatc ggtcgttgaa
    25141 cgtcccgcct aatctgctcg ataagcgcca caacgcatga gacaaattgt tgatatgtcg
    25201 acttttgcgt cgggcagcgg taacggcgaa tctcccagcc atgcgcatcc atgacgacgg
    25261 cggcgatttt ggtcccacca atatccaacc ccagatagtg catatctcgt tccttgttgc
    25321 gtaaaaggcg ggtcaccccg cccttctggc ttagtacagt gatgctttac cgagcgaacc
    25381 gaacagtttc attttgtggc gaatgacttc ccgcgctgcg ttaatcgcat ccggatagat
    25441 gacgttcggg tcccaccagc tctctttagc gagaatttca cgcactttct ggaaataggc
    25501 gtatttcata tcgctggaga tattgatttt gccgacgccg agcgtaactg attcagcaat
    25561 ctccgcgtcc gggtttgccg aaccaccgtg tagcaccagc ggaatatcaa tacgtccggc
    25621 aatgtcgcgc aaaatgtcca tttgcagctt cggctgcatc ccttttggat agatgccatg
    25681 cgctgtacca atcgccacag ccagtgtgtc ggcacctgta cgagcaataa aatcggcggc
    25741 ctgcgccgga tctgtgtagg tcacttgtga aacaccacct tctaccgacg ttcctgtttg
    25801 tccgatggtt cccagttcac cctcaaccga tactccaacc gcgtgagcga ggcgaaccac
    25861 ttctgcggtc agcgccacgt tttcttcata tggcagcagc gaaccgtcga tcattactga
    25921 ggtaaatccg cagcgaatcg cacgcaaaac atgctctaca gaggcaccat gatcgaggtg
    25981 cagtacaaac gggaccggac tacgctgggt aatttcgcga acgtgagcga aaaaggcatc
    26041 gcccacaaaa tcatgttcgc tgggatggat ggcaataatc gtcggagtat ttgtcgcttc
    26101 tgcttcatcc acgactgcgc gaaggaaaca gttatccgcc acattaaatg cgccaatggc
    26161 aaagccgtgc tggcgggtag gttgcagtaa ttctttcatc gaaactaaca tgttaaatct
    26221 ccagtttcag cgtgtagtcc gccctgccgg tgcgttattg cactggcata aaggccatga
    26281 cagaaaatga atcaggcttt gccgatagcg ccgctcgcct gtaaatacgg tgtaatcgct
    26341 tcttcgaagg ccagttccat agctttcagc gcgtccacca gttcaatgga tatatcgtga
    26401 cagagggtat gcaggagcgc ggatttcccc cgttcaaaaa tggccgcccc cacgttgatt
    26461 tttgctaccc ccagcgtcgt acagagctgg agctgatcga acggcgtacc gctaccgcca
    26521 tgtaatgcca gaggcacgct cgagcagcgc gaaatctccg ccagacgctg aaaatcaata
    26581 gcaggtacaa caccaggcgc gtacatgccg tgcgccgtgc ctaccgagac ggccagtaga
    26641 tcaatgcctg tctgcgcgat aaacgtttcc acatcagtgg gttgcgtcat caggtctgca
    26701 ttggcggcct ggtcgtaacg cggcgcatct gccagatggc ccagttcgcc ttccacgcag
    26761 agctgcacgc tggcggcgag ttccaccacc gccctggtct ggcgcacgtt ttcctccagg
    26821 ggaaaagcag aagcatcaat cattaatccg ctgaaaccag cacgaaacgc ctgacgaata
    26881 cagtccggat ttttgccatg atcaagcgcc agagcgaccg gtactgaggc agattccgcg
    26941 agcgttttaa ccactgccgc cgcgacgggt gcaggaaaat gcgccagatg cccctgatag
    27001 agattgagaa tgatcggagc gcgattcttc tccgccacgc ggatagctgc gtgagcactt
    27061 tccagattca tacagttgat cgccagcaaa gcgtaatgtt catgccaggc tttcatcacc
    27121 atacttttca tatcggcaaa catagggcct ccggttattc gagggtgaaa gtgatctctt
    27181 catctttcag cttttgatcg ctcagttcgt caaactcttc gtcgcgttcc gttaccggtt
    27241 tcttccagag tgaaagcatc acgccacaaa tcaccgtgcc tatacccagc gccaggcaaa
    27301 acagcagagg atgggtgaac agaggcacaa caaacatgcc gccatgcgga accggactct
    27361 ccacgcccca caccatgatt aagccgcccg cgaccgccga tgccaccaca caagatccca
    27421 ctactcgcag caggtcacgc gcggcgatcg gaatgactcc ttcagtgatc atgcagatcc
    27481 ccatcggaaa cgcggctttc agcgcctcgc gctccgcacg ggtgtattta tggcgtgtca
    27541 gtaagaacga aagtgtgatg ccaaacggag ggatgattga gccgacaaat ttcacggctt
    27601 ctggtccgta gacgccactg accagcaagc catcggaaaa cagcgacatg gttttgttga
    27661 ccggaccacc gaaatcgaag gttgccatcg cgccaagtat ggcccccatc aggaagcgcg
    27721 atccgccctg catcgactcg agcaaatgaa tcagtgcttc ttgtaaccag gcgatgggtt
    27781 taccaatcag cgtcatcatt aacagcccac cgataatggt gcttaatacc ggcaacacca
    27841 tgattggcat gagtccttgc atcgactgcg gcaggcggat atagcgttta agtagcaaaa
    27901 tggtgtaacc caccaggaaa ccacccaaca taccgccaat aaatccggta tggatttgcc
    27961 cacagagaaa gccgacaatc aggcccgggg caaatccggg acgatcggca atagaatagg
    28021 caattgccgc gctaatcagc ggcacaatca gccccattcc ccagccgcca atctgattaa
    28081 gcatccacgg gattgttcct gttttttcac caacatctgt accaccaaga acttgcccca
    28141 acgcaataca tatcccggcg gccacaataa ggggtatcat ccacgagatg ccggttaata
    28201 aatgtttttt aatttcctgt gaaaccggaa ttttattttc tttcatatta agatccctcc
    28261 agtataagga tcatttgttt cccagtgatt tttcgacctt ttcaagaaat tgaacgggcg
    28321 atttaatgac gatctctgtc tttactcgca caatacgttt attggtgaag cgttcttctc
    28381 cttttatttt cacatctaca gcaagaatca cgacgtcagc agcagagata tctgccgcct
    28441 gtaattcatt ttctgtacca atggttccct gggtttcaac tttaatggta tgacccagag
    28501 cttttgctcc tttgattaac ttttcacgag caatataggt atgagcaata cccgccgtac
    28561 aggcggcaac acagacaata ttcatgacat actccctgtt atttagcaga catcggattg
    28621 tgcatactga ctaaagagat caatgatttt ttgcggactc gtctcaacta acaattgttc
    28681 tatgatgtca tcatccgcca gcgcgcccgc gacctgggaa agtaaacgga tatgggtggt
    28741 attttgatct tcaagacgta ccgcaaataa aataatacag cgcacgctac tgccatccag
    28801 cgtttcccag ggaatatcct gccgggtacg gccaattgcc agcgtagtat taatcacagc
    28861 tgaagattta ccatgcggga tcgcaatatg attttcaaaa ccagtcgagc cttccgcttc
    28921 gcgctgccag acatctttaa taaaggtttc ccgacaatcg atcgcaccat cttttttgag
    28981 aagattagtt aattcttcaa tcgcctcctc tttattcgtc gcttgcatat ctaaaataac
    29041 gcgattcgta ttcaggattt ttgttatgtc cattcgtcat acctcataaa attctgacat
    29101 taaatttcaa cgacccgaga tgtcgcatga tgtaattttt ccgtaatcac catttcagtg
    29161 acccgcttaa tatcggcatc attaaaaaaa gcggtaacat aagcgaccgg catagtgact
    29221 gtaggcaaat taatggtcga aataatcagt tcgatattgt caggcaaaac ctgtgataaa
    29281 ttcgctgctg aaataacatc aacaatcgtc cattcaggaa atgctcgcag aatacggctt
    29341 ttcagtagat gcgaagttcc gattccggtt gaacagacca gtaatacgcg tttacgggca
    29401 atttgccgct ccatcgcagc ctgaaaatga acggtcaggt aacctacttc atcctcgctc
    29461 actgcattct caccccaggt tttaaatacc tgattcacca catattgcgt caaccgccag
    29521 acatccgcta gttctgcttt aatatcttcc aacaatggat tacggatatg aatacgataa
    29581 tttagtcggt taatcagcgg tttaatatgc accaataagc catcatataa tgcgctgtct
    29641 tcactaaaat cgcagtccac gatgtcactg aataccgtaa ttaaacgcca ggtaatcaga
    29701 cgcgcttcat tgctggcctg catatgtgaa ataatgctaa catctttttg tccatcaatc
    29761 gccacacctg atgaaatgat atattgataa ataaaccaga cttcatcatc gggcaatgta
    29821 tgtgcaattc gttgttctat cttatgaatc atctgactgg caacattaaa gatattttca
    29881 tcatattgcc tggtttgatc ttcttctctt gataacgcat tcccgtgcgt gttgcgatac
    29941 atcataataa ggatgtgagt aaaaatgtta acgtaataag gttctcccaa agaccaactt
    30001 aattcatttt ccatatccag taacaatgac tggacaaata acacctcttc ttctccaaaa
    30061 taatggacta atgctttata gcttccggga tccagacgtg aatacacgac accttgcggc
    30121 tcattatggt taataatgcc gttaatcagt aatgccattg cctgtcggac ttgcccttca
    30181 ctaccttcaa tatgcgtacc acttggactg cggatcaacg ataaccccaa cggcgcaagc
    30241 caggactcaa ttactctcag gtcattaaca atagaggctc cgctgataaa gtagcgttct
    30301 gacaatttac tgatcgacgt ttcattcggt gtttcactta ataactgcga cgctatttta
    30361 acgcgcctgg aattattcat tacaccatca atctcttcac attctgccac caacaaatga
    30421 tcaagatgca gcaaatcagt aatattctcc gcgcttaaca tcacacccgc gccagcacgc
    30481 ttctcaacat ttattctcca ttgtcccagc cattgctcaa gtaaacgtaa atcacgttga
    30541 atggtttttt ccgaaacatt taaatagccg gcaatcgtaa caatagtcgt atattccctt
    30601 cgaggtaaaa ggaatcgtaa taatctgttt tgtcgcgagg taatcatttg cattttttac
    30661 ccttatcaca gattaagaag cctcatgagg aagagcttca gttgccatct ctgagggtaa
    30721 atataaagca gaataatttc acaacaatac taaaatctgt ccgtaatccg gacagtgata
    30781 aaacaaagca aggcggttaa gggatatttt tcagcacaga tcacattttc gcgatgaata
    30841 atcgtgatca tgctcgcaaa tacgatggaa gaaatattta ttaccagatg tgagattatt
    30901 taccggatac gacacaaaac cattcgtacc cggcattcca tcaatagatt acaacgtaat
    30961 tgtcagcgca ttcccttgag gcgtcaccat cagcccctgc tcactttcag cctgcgaacc
    31021 gccttgcaga ccatttactt tcacaatatt gcgcaagcac agtgtccagt tcttcgcctc
    31081 gcccgcacca gtcacagtaa tcgtgttacc agtacgtgtt gcttttagag taaagatcac
    31141 cgaaccgtca gcagcgggca tttcacagac ggcttcatgc ccgtcttgta gattaaagag
    31201 ctggaatgcc gtgccttcgt gccacgcgta atcgggacgt tgctcgttgt tgcccagcgc
    31261 cagtagggtg ttatcacgca cataaacggg cagactcagg aagctgtgct gctgtttatg
    31321 ccagcgacta ccatcgagtt catcgttgtg ccacaggtgt gtccagcgac cttcaggcaa
    31381 gtagaactgc acatcgcccg cttcagtgaa cacaggcgca accatcacgt tgtcgcctaa
    31441 catgtattga cggtcaaggt aatcacaagc cggatcgtcc ggaaactcca tcatcatggc
    31501 ccgcatcatc ggcgtacccc gcgcgttcgc acgagcagct tcacgataca gatacggcat
    31561 catgcggcat ttcagttgcg tgaagaagcg caccacatca caggactcat catcgtaggc
    31621 ccacggcaca cgataagatt tgctgccatg taaacggcta tggctggaga gcaaaccaaa
    31681 cgcgcaccag cgtttgtaaa catgcgccgg cgcggtattc tcaaagccgc cgatatcgtg
    31741 gctccagaag ccaaaacctg aaaggccaat agacaaacca ccgcgcaggc tttccgccat
    31801 tgattcgtag ttagcgtaac agtcgccacc ccagtgtacc gggaatttct gcgcaccgac
    31861 ggaagccgag cgggcaaaca agacagcttc ttcctcacca acggtgtcct tgagcacgtt
    31921 ccacaccagt tcgttgtaga tgtacgcata atggttatgc attttctgcg gatcggaacc
    31981 gtcaaaccat tgaacatcgg ttggaatacg ttcgccaaag tcggtcttaa agcaatcaac
    32041 gcccatcgcg accagacctt tcagtttgtc ggcgtaccat ttgcaggcat ccggattggt
    32101 aaagtcataa attgccagac ctggctgcca tttatcccac tgccataacg aaccgtccgg
    32161 gtgtttgagt aaatagcctt tctcttgtaa ctctttaaag acaggggatt tttgcccaat
    32221 ataggggtta atccagacgc agatttttag ccctttcgct ttcaggcggc ggatcatccc
    32281 ttccgggtcc gggaaagtca gcgggtccca ctcaaaatcg caccactgga aggctttcat
    32341 ccagaaacag tcaaagtgga aaacatgcag cggcagattg cgttccgcca taccatcgat
    32401 aaagctgttt accgtcgctt cgtcgtagtt ggtggtaaat gaagtggtta gccacaggcc
    32461 gaaggaccac gcgggcggca gcgccggacg accagtaaaa cgggtataac gatcaagtac
    32521 cgctttcggc gtcgggccgt cgataacaaa gtattcgaga tattcactct caacgctgaa
    32581 ctgcactttg gagactttct ccgatcccac ttcaaaagag acgcattgag gatgattgac
    32641 cagtacccca taaccacggt tagtcatata gaacgggata tttttatacg cctgttcagt
    32701 acttgtgccg ccgtcccggt tccaggtctc taccgtctgg ccattgcgca ccagggcagt
    32761 aaagcgctct cccagaccgt aaactgtttc gccaacgcca agatccagcc gctcaaacat
    32821 ataatttcgt tgattattcg tgtcctgcac gtagccatta tttttcacct gactaccggt
    32881 aatacgttcg ccgttgcgca gaaaatccag tgaccagaac tcacctttgc tgacacgcgc
    32941 gcttaagttg ccacttttaa actcagcata acgttctgtg ttttcgattg tgaccttcac
    33001 atcctgcaaa atattgagcg gataatgagg accgttattc agcgccccct gaaaatgctc
    33061 aatccgtaca ccaacaatac cttcctgtgg ggagaaaaag cgcagcgtaa ataaaggtgt
    33121 atcaagctgc caggtacgtt cacgcacatc acggggggca gcatagacca ccatttcatt
    33181 accctgctgt tcaacctcga acacctgaag cgggtgaatc aaattgaggc caggttgaat
    33241 caaccagttt ccgtcgctaa ttttcattgc cgttccttag ttctgcactt cttgagaggt
    33301 gaattgttgc tggcaacggc gtttgccctg cgccagctgt tccataacgg ttttcagatt
    33361 gtgcgtcgtg agtgagtagt agcgtttagc gataatcgcg ctcagcaagt aacagatcgc
    33421 cggaacaatc gtgaatagcg cgataatgat gctaatcgtg gcgctgttct gcgctttttc
    33481 tgccgcatca tatccgccat aagccagcat ccagccgata agagcgccgc cgaaggccaa
    33541 ccccagcttg agcacaaaca gcgtgccagc aaaactgatc ccggtcaggc gtttaccatt
    33601 gcaccactcg ccgtagtcga cggtatcgga catcattacc cactggatag gtgtcaccag
    33661 ttgatgcaat acaccaatca cgaagatgaa gacaaacata gtgatgctgg cctgcatggg
    33721 aacaaagaac atcgcgaggc taatcactgc cagcagggcg ttcgtccacc agaagatagt
    33781 gactttacat ttccagtcgg tcagcggttt tgccagtgcg gaaccaatca ggttacccac
    33841 gcaataagtg gtgagaaaag cgacaaacac ttccggcgta cccaaaatcc atgtgacgta
    33901 atacatcatc gctccaccgc gtacgcacac cgccagaata ttgaaaatgg ttagtaaacc
    33961 gacaatccgc cactggtcgt tttgccagat atcacgtaaa tcttcccgca tagacgtcgt
    34021 tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa cagaatgcca gcatcatgaa
    34081 tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg agtggtttat tatcaccacc
    34141 aattaaatta accagcggca tcatcagcac agtagagagc atgcctcccg cggtcgccag
    34201 cacaaaacgc caggattgca gcgagatacg ctgagtcggg tcattggtga ttacaccacc
    34261 caatgcgcag taagggatat taacgatggt ataaagtaag gtaagtaggg tgtaagtaat
    34321 tgctgcataa atcatttttc cgttcatact gagatctggc gtgctatagg ccagtacaca
    34381 gacgatccca aatggcagtg cgccaaacag tacccacgga cgaaatttac cccagcgaga
    34441 gcgcgttcga tcggccaaca accccatgca aggatcggaa atcgcatcca gtgcacgagc
    34501 gaccaaaaac atggttccga caaatccggc aggaatgcca aaaatatcgg tataaaagaa
    34561 catcatatat aacattacgt tatcgaaaat aatgtggctg gcggcgtctc ccatgccata
    34621 accaattttc tctttaacgg acaacacttc actcttcatc tgcttttcct tgatgctata
    34681 ggggtgctga gaccgataac gcattttgta aaccatccct gcgagagcga atattccgat
    34741 atctggttat taatttatga ttcttgtttt atgtgatcgt ggtagcgtta atttcgctca
    34801 tatatcat
//
LOCUS       DAZUCC010000024        34479 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZUCC010000024 DAZUCC010000000
VERSION     DAZUCC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.024"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..702
                     /locus_tag="ACHY09_001048"
     CDS             <1..702
                     /locus_tag="ACHY09_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFS3884051.1"
                     /translation="VWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAF
                     DDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKASASLAALWQVV
                     NQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEK
                     QLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFR
                     GMAKRIARLAEQSTD"
     gene            801..1814
                     /gene="ybjS"
                     /locus_tag="ACHY09_001049"
     CDS             801..1814
                     /gene="ybjS"
                     /locus_tag="ACHY09_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS3884052.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1811..2641)
                     /gene="amiD"
                     /locus_tag="ACHY09_001050"
     CDS             complement(1811..2641)
                     /gene="amiD"
                     /locus_tag="ACHY09_001050"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFS3884053.1"
                     /translation="MRRVFWLVAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTLVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2638..2961)
                     /gene="ybjQ"
                     /locus_tag="ACHY09_001051"
     CDS             complement(2638..2961)
                     /gene="ybjQ"
                     /locus_tag="ACHY09_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS3884054.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            3087..3602
                     /gene="ybjP"
                     /locus_tag="ACHY09_001052"
     CDS             3087..3602
                     /gene="ybjP"
                     /locus_tag="ACHY09_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS3884055.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3820..4548
                     /gene="artP"
                     /locus_tag="ACHY09_001053"
     CDS             3820..4548
                     /gene="artP"
                     /locus_tag="ACHY09_001053"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFS3884056.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            4566..5297
                     /gene="artI"
                     /locus_tag="ACHY09_001054"
     CDS             4566..5297
                     /gene="artI"
                     /locus_tag="ACHY09_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFS3884057.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            5304..6020
                     /gene="artQ"
                     /locus_tag="ACHY09_001055"
     CDS             5304..6020
                     /gene="artQ"
                     /locus_tag="ACHY09_001055"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFS3884058.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTVLRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            6020..6688
                     /gene="artM"
                     /locus_tag="ACHY09_001056"
     CDS             6020..6688
                     /gene="artM"
                     /locus_tag="ACHY09_001056"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFS3884059.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            6914..7645
                     /gene="artJ"
                     /locus_tag="ACHY09_001057"
     CDS             6914..7645
                     /gene="artJ"
                     /locus_tag="ACHY09_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFS3884060.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7674..8801)
                     /gene="rlmC"
                     /locus_tag="ACHY09_001058"
     CDS             complement(7674..8801)
                     /gene="rlmC"
                     /locus_tag="ACHY09_001058"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFS3884061.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLSVKHMWDLFCGVGGFGLHCAMPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYCIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(8842..9330)
                     /gene="ybjO"
                     /locus_tag="ACHY09_001059"
     CDS             complement(8842..9330)
                     /gene="ybjO"
                     /locus_tag="ACHY09_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFS3884062.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(9390..10235)
                     /gene="potI"
                     /locus_tag="ACHY09_001060"
     CDS             complement(9390..10235)
                     /gene="potI"
                     /locus_tag="ACHY09_001060"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFS3884063.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDNAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(10232..11185)
                     /gene="potH"
                     /locus_tag="ACHY09_001061"
     CDS             complement(10232..11185)
                     /gene="potH"
                     /locus_tag="ACHY09_001061"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFS3884064.1"
                     /translation="MSTLEPAAQPKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(11195..12328)
                     /gene="potG"
                     /locus_tag="ACHY09_001062"
     CDS             complement(11195..12328)
                     /gene="potG"
                     /locus_tag="ACHY09_001062"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFS3884065.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPADGFNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(12423..13535)
                     /gene="potF"
                     /locus_tag="ACHY09_001063"
     CDS             complement(12423..13535)
                     /gene="potF"
                     /locus_tag="ACHY09_001063"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFS3884066.1"
                     /translation="MTALNKKWLSGLVTGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(13886..14362)
                     /gene="ybjN"
                     /locus_tag="ACHY09_001064"
     CDS             complement(13886..14362)
                     /gene="ybjN"
                     /locus_tag="ACHY09_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFS3884067.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(14450..15352)
                     /gene="rimK"
                     /locus_tag="ACHY09_001065"
     CDS             complement(14450..15352)
                     /gene="rimK"
                     /locus_tag="ACHY09_001065"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFS3884068.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(15413..16135)
                     /gene="nfsA"
                     /locus_tag="ACHY09_001066"
     CDS             complement(15413..16135)
                     /gene="nfsA"
                     /locus_tag="ACHY09_001066"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFS3884069.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENNYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(16119..16406)
                     /gene="ybjC"
                     /locus_tag="ACHY09_001067"
     CDS             complement(16119..16406)
                     /gene="ybjC"
                     /locus_tag="ACHY09_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFS3884070.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            16566..16823
                     /gene="grxA"
                     /locus_tag="ACHY09_001068"
     CDS             16566..16823
                     /gene="grxA"
                     /locus_tag="ACHY09_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFS3884071.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(16853..17230)
                     /gene="ybjM"
                     /locus_tag="ACHY09_001069"
     CDS             complement(16853..17230)
                     /gene="ybjM"
                     /locus_tag="ACHY09_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFS3884072.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            17500..19185
                     /gene="ybjL"
                     /locus_tag="ACHY09_001070"
     CDS             17500..19185
                     /gene="ybjL"
                     /locus_tag="ACHY09_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFS3884073.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(19361..19897)
                     /gene="rcdA"
                     /locus_tag="ACHY09_001071"
     CDS             complement(19361..19897)
                     /gene="rcdA"
                     /locus_tag="ACHY09_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFS3884074.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEEMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            19981..21189
                     /gene="ybjJ"
                     /locus_tag="ACHY09_001072"
     CDS             19981..21189
                     /gene="ybjJ"
                     /locus_tag="ACHY09_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3884075.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYVGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            21189..22004
                     /gene="ybjI"
                     /locus_tag="ACHY09_001073"
     CDS             21189..22004
                     /gene="ybjI"
                     /locus_tag="ACHY09_001073"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFS3884076.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFSTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            22089..22373
                     /gene="ybjH"
                     /locus_tag="ACHY09_001074"
     CDS             22089..22373
                     /gene="ybjH"
                     /locus_tag="ACHY09_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFS3884077.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(22414..23646)
                     /gene="mdfA"
                     /locus_tag="ACHY09_001075"
     CDS             complement(22414..23646)
                     /gene="mdfA"
                     /locus_tag="ACHY09_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFS3884078.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHVWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            23931..24527
                     /gene="ybjG"
                     /locus_tag="ACHY09_001076"
     CDS             23931..24527
                     /gene="ybjG"
                     /locus_tag="ACHY09_001076"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS3884079.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWSMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            24585..25343
                     /gene="deoR"
                     /locus_tag="ACHY09_001077"
     CDS             24585..25343
                     /gene="deoR"
                     /locus_tag="ACHY09_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFS3884080.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(25390..26592)
                     /gene="dacC"
                     /locus_tag="ACHY09_001078"
     CDS             complement(25390..26592)
                     /gene="dacC"
                     /locus_tag="ACHY09_001078"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFS3884081.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            26839..27465
                     /gene="gstB"
                     /locus_tag="ACHY09_001079"
     CDS             26839..27465
                     /gene="gstB"
                     /locus_tag="ACHY09_001079"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFS3884082.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(27462..28577)
                     /gene="yliI"
                     /locus_tag="ACHY09_001080"
     CDS             complement(27462..28577)
                     /gene="yliI"
                     /locus_tag="ACHY09_001080"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFS3884083.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESDARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(28688..29071)
                     /gene="bssR"
                     /locus_tag="ACHY09_001081"
     CDS             complement(28688..29071)
                     /gene="bssR"
                     /locus_tag="ACHY09_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFS3884084.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            29284..30609
                     /gene="rimO"
                     /locus_tag="ACHY09_001082"
     CDS             29284..30609
                     /gene="rimO"
                     /locus_tag="ACHY09_001082"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFS3884085.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            30835..31221
                     /locus_tag="ACHY09_001083"
     CDS             30835..31221
                     /locus_tag="ACHY09_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3884086.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVIQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            31211..31540
                     /locus_tag="ACHY09_001084"
     CDS             31211..31540
                     /locus_tag="ACHY09_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3884087.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(31610..32938)
                     /gene="dgcI"
                     /locus_tag="ACHY09_001085"
     CDS             complement(31610..32938)
                     /gene="dgcI"
                     /locus_tag="ACHY09_001085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS3884088.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQLPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(32946..34479)
                     /locus_tag="ACHY09_001086"
                     /pseudo
     CDS             complement(32946..34479)
                     /locus_tag="ACHY09_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT         8693 a         8914 c         8407 g         8465 t
ORIGIN      
        1 gtgtggtttc tcaatgtgat tacttccgta ccgcccacca ccgccagggc gttgattcag
       61 gggctgaaac atgatctgct ggcggatgac accgcgctac gtgcactcat cccacaacgg
      121 ctgatcgctt tcgatgacgc ggtacgtcgc accctgaaag aagaagaaaa gctggtcaac
      181 tccagcgact ggggctacga cgctcaggcc tttgcccgct ggcgaccgga gtacggttat
      241 ttcgccaaac aggcggggtt taccgttaaa gcgtccgcca gccttgcggc tttatggcag
      301 gtggtgaatc aaatcggcgg taaagagcgt tatttctttg gcaatatttt gtggcagaca
      361 cgggcgttga tggaccgcgc gatcggtcat aagctggcga aaggccgccc ggagcgtgaa
      421 tatttgcaga ccggcgatgc ggtggatagc tggaaagtga ttgtcgttga accggaaaaa
      481 caacttacgc tgttatttgg catgaaagcg ccggggctgg gacgactgtg ttttaccctg
      541 gaagataaag gtgactatcg tactatcgat gttcgcgcat tctggcatcc gcacggtatg
      601 ccggggctgt tttactggtt attgatgatc cccgcgcatc tgtttatttt tcgcggaatg
      661 gcaaaacgaa tcgccagact ggcagaacaa agcacagatt aatcgacaaa acctgcgtta
      721 ttctttcatg tttcccattg catcatgggg gaaatcacgg aagaatgcgc acgttatttt
      781 ctcaacaaca gtggattgat atgaaggtac tggttaccgg cgccaccagc ggcttaggtc
      841 gaaacgcggt agagttttta tgccagaaag gcatcagcgt gcgagcgacc ggtcgcaacg
      901 aggcaatggg taaattgctg gaaaaaatgg gcgcagagtt tgttccggcg gatctgaccg
      961 agctggtctc atcacaagct aaagtgatgc tcgcgggcat tgatacgctg tggcactgct
     1021 ccagctttac ctcgccctgg gggacacaac aggctttcga tctggctaac gttcgcgcca
     1081 ctcgccgcct gggtgaatgg gctgtcgcct ggggtgtgcg taactttatt catatctctt
     1141 caccctccct gtacttcgat tatcaccacc atcgcgatat taaagaagat tttcgccctc
     1201 accgcttcgc caacgagttt gcccgtagca aagcagccag cgaagaggtg atcaatatgc
     1261 tttcgcaggc gaatccacaa acgcgcttta ctattctgcg cccacaaagt ctgttcggac
     1321 cgcacgataa agtctttatt ccccgtctgg cgcatatgat gcaccactac ggcagcattc
     1381 tgttaccgca tggcggcagt gcgctggtcg atatgaccta ctatgaaaat gccgtgcacg
     1441 ccatgtggct ggcaagccag gaagcctgcg ataagctacc ttccgggcgt gtgtacaaca
     1501 tcaccaacgg cgagcatcgc acactgcgca gcatcgtgca gaagctgatc gacgagttga
     1561 atattgactg tcgtattcgt tccgtcccct acccgatgct ggatatgatc gcccgcagca
     1621 tggagcgttt aggccgcaag tcagcaaaag agccgccgct gacccactac ggcgtctcca
     1681 agttgaattt tgactttacg ctggatatta cgcgcgcgca ggaagagtta ggttatcagc
     1741 cggtcatcac cctggatgaa ggtatcgaga aaactgccgc ctggctacgc gaccacggaa
     1801 aactgccgcg ctaatcctgc ccgtatttct ccagcaacgc ttcggcaatc gcctgagttt
     1861 ctgcatccgc ttcgccgttg tataacgtcg ggcggaaatg catctggaac gccataatca
     1921 cgcgccgctg ctcgcgcggt gtcatatcag gtttaacgtc ataaccgtag cgcgccaaaa
     1981 gctccagtaa tgatgcggta tctaccagtg tgtgcggcgc gcgcccggca aggtaaaagt
     2041 tcacccgctg cgcatccggc caggcaccaa tcccctgctg cgccagttgt tgccagggga
     2101 ataacggccc cggatcgtct ttgcgctgcg gcgcaatatc cgcatgcgcc actacgtttt
     2161 ccggcttgat gtgataacgg gcaataatat ctttcgccag cggaataagc gcctgaatct
     2221 gtgcctgttc aaacggggca aaatatttca ctccggatga tttttgccag ccacggtttt
     2281 ccagctcaat gccaatagag gtgtcgttaa gacgcgttgc cccgcgccag gcgctaatcc
     2341 ccgcatgcca ggccagttcc tgttccggca ccagttgcca gatgcgcggt ttaccgttgt
     2401 atcgcggtgg taccgcaggg accagataat gcgagctgac ctgcttatcg gtcagtgtcg
     2461 ccagcgagct atcaaaatca tctgcggtgt agtggataac cagcacttta atgcgcggat
     2521 acgccgcctg cgcctggcgt cgggtatcaa gctgatatcc ctctttctcg acaatgcctt
     2581 tttcgcctgc acaccctgcc agtaacagag cgacagcgac cagccaaaag actcttctca
     2641 tcgacgcgtt ttcaccgccg taccgctgac gctaaccatc agcatactgc cattttgccc
     2701 gaccgtttcg tagtcgatat caataccgac tacggcatca gcccccagcg cccgggcctg
     2761 atcgccgagc tcctcaaagg cgatctcccg cgctttacgc aattcttttt cataagcccc
     2821 ggagcgtccg ccaacgatat cgcggatacc ggcaaagaaa tcacggaaaa tattggcacc
     2881 taaaatcgct tcgccggtca ccacaccgca atattcgacg atggtctggc cttccagagt
     2941 tggggttgta gaaaattgca tgatcgatct ccttgtttgc taagccttcg atcttcaaag
     3001 cattatcgga ctgatacgct attattgaaa ggatatcatt attatcgtag aacgctttca
     3061 aggtgttcgc ttataaggaa atcatcatgc gctatagcaa attgaccatg cttatccctt
     3121 gcgcactgct actcagtgcc tgtaccaccg tcacgccagc ttataaagat aacggccccc
     3181 gcaccggttc ctgtgtccag ggggggcctg atagcgtggc tcaacagttc tatgactacc
     3241 gcattcaaca tcgcggcaat gacattaccg ctctgcgccc ctatttgagt gacaaactgg
     3301 caaccctact tagcgatgcc agccgcgata atagtcatcg tgaattgtta agcagcgatc
     3361 cgttctccag tcgaaccacc ttgccagaca gcgcccacgt tgccagtgca tcgactatcc
     3421 ctaatcgtga tgcccgtaac attccattgc gcgtggatct caagcagggt gaccagggct
     3481 ggcaggatga agtgttaatg attcaggaag gccagtgttg ggtcatcgac gatgtgcgct
     3541 acttaggcgg tagcgttcat gccacagcgg gtacgctccg tcagtccatc gagaatcgct
     3601 aacgtcttga tcaaccaata aaccccgtaa attgtacggc atttcgtgag gaatgccgac
     3661 atttatgctc gccgaccacc gcccccgtta ttttgtgcta tgtttattga ataatgcgct
     3721 ttgcttttaa cttttaaagc agaaatattg cataattatt ctgtcaaagg tactatcttc
     3781 gacctcaata gctatcagac tgccagtata cgagtgtcaa tgagtattca attaaacggc
     3841 attaattgct tctacggcgc gcatcaggcg ctgttcgata tcacgctgga ttgcccacag
     3901 ggcgaaacgc tggtgttact tggccccagc ggcgcgggta aaagctcgct gctgcgtgta
     3961 ctcaatctgc ttgagatgcc gcgctccggt acgctcaaca ttgcaggcaa ccatttcgat
     4021 ttcaccaaaa ccccctctga caaagcgatt cgcgatttgc gtcgtaacgt tggcatggtg
     4081 tttcagcaat acaacctgtg gccgcatctg accgtgcagc aaaacctgat tgaagcgccc
     4141 tgtcgtgtac tggggttgag taaagatcag gcgctggccc gtgcagaaaa actgctggaa
     4201 cgtctgcgtc tcaaacctta tagcgatcgt tacccgctgc atctttctgg aggtcagcag
     4261 cagcgtgttg ctattgcccg tgcgttgatg atggaaccgc aggtactgct gttcgatgaa
     4321 ccgaccgccg cactggaccc cgaaattacg gcacaaatcg tcagcatcat tcgtgagctg
     4381 gcagaaacga atattaccca ggtgatcgtc acccacgaag ttgaagtggc gcgtaaaacc
     4441 gccagccgag tggtgtatat ggaaaacggt catatcgtag aacaaggcga cgcgagctgc
     4501 tttaccgagc cgcaaaccga agcatttaaa aactatctct ctcactaaga ttcgggataa
     4561 caacaatgaa aaaagttctg attgccgcgt taattgcagg ttttagtctt tccgccacag
     4621 cagccgaaac cattcgtttt gccaccgaag cctcctatcc tccgtttgaa tcgattgatg
     4681 caaacaacca gatcgttggt tttgatgtcg acctggcgca ggcattgtgt aaagagattg
     4741 atgcaacctg cactttctct aaccaggcgt ttgacagcct gatcccaagc ctgaaattcc
     4801 gtcgcgtaga agcagtgatg gcgggcatgg atatcactcc ggagcgtgaa aagcaggtgc
     4861 tgtttaccac cccgtactat gacaactctg ccctgtttgt gggtcagcaa ggcaaataca
     4921 ccagtgttga tcagctgaaa ggcaaaaaag tcggcgttca gaacgggacg acacaccaga
     4981 agttcattat ggataagcat ccggaaatca ctaccgtgcc atatgacagt taccagaacg
     5041 caaaactgga tctgcaaaac ggtcgtatcg acagcgtatt tggtgacacc gcagtggtaa
     5101 ccgaatggct gaaagataac ccgaaactgg cggcagtggg cgataaagtg accgataaag
     5161 attacttcgg tactggcctc ggcatcgcag tacgccaggg caacactgaa ctgcagcaga
     5221 aactcaacac tgcgctggaa aaagtgaaga aagatggcac ttacgaaacc atctacaaca
     5281 aatggttcca gaagtaattc ctgatgaatg aattttttcc tttagcaagc gccgccggga
     5341 tgaccgtcgg ccttgccgtt tgtgcattga ttgtcgggct ggcgctggcg atgttctttg
     5401 cggtatggga gtctgcaaaa tggcgtcctg tcgcgtgggc aggttcagcg ttggtgacgg
     5461 ttctgcgcgg cctgccagaa attctggtgg tgctgtttat ctattttggc tcctcgcagc
     5521 tgctgctgac gctttcggat ggcttcacta tcaatcttgg gttcgtgcag atcccggtgc
     5581 agatggacat tgagaacttc gacgttagcc cgttcctttg tggtgtcatc gctctgtcac
     5641 tgctgtatgc cgcctatgcc tcgcaaacgc tgcgcggcgc gttgaaagcg gtgccggtgg
     5701 gccagtggga atctggtcag gcgctggggc tgtcgaaatc ggctatcttt ttccgtctgg
     5761 tgatgccgca gatgtggcgt catgcgctgc cgggtctcgg taaccagtgg ctggtgctgc
     5821 tgaaagatac cgcgctggtc agtttgatta gtgtgaatga tttaatgcta caaaccaaaa
     5881 gcatcgctac ccgtacccag gaaccgttta cctggtacat tgtggcggcg gcgatttacc
     5941 tggtgatcac cctgctcagt cagtacattc tcaaacgcat tgacctgcgc gcgacacgtt
     6001 ttgagcggag gcccagctaa tgtttgagta tttacccgaa ctgatgaaag ggttgcacac
     6061 cagcctgacg ctaaccgttg cctcgctgat tgtggcactg attctggcat tgatttttac
     6121 catcatcctg acgctgaaaa cgccggtgct ggtgtggctg gtgcggggct atatcacgct
     6181 gtttaccggt acgccgctgc tggtgcagat cttcctgatt tattacggac cgggccagtt
     6241 cccgacgttg caggagtatc cggcactgtg gcatttgttg tcagaaccgt ggctgtgtgc
     6301 gctgattgcg ttgtcgctga acagtgcggc gtataccacg cagctgtttt acggtgcaat
     6361 tcgtgcgatc ccggaaggtc agtggcagtc ctgtagcgcc ctgggaatga gcaaaaaaga
     6421 tacgctggcg atcctgctgc cgtatgcctt taaacgctcg ctctcttctt attccaacga
     6481 agtagtgctg gtgttcaaaa gtacctcgct ggcctacacc attacgctga tggaagtgat
     6541 gggatacagc cagttgttgt acggacgcac ctacgatgtc atggtgttcg gcgcggcagg
     6601 gattatttat ctggtcgtta acggcctgct gacgctgatg atgcgtctga tcgagcgtaa
     6661 agcgctggcg tttgagcgaa ggaattaatt tggctgcgtg ctgatgccgg atgcggcgcg
     6721 gacgccttat ccggcctaca tagtgcataa attcttataa tgaagacgga caactcacta
     6781 agttgtccgt ctttttttat ttcatttaaa ttatttaatc atatttattg catataaatt
     6841 cacttgatgg cattgttatc ccatgccgca gacacggcca aaaatcataa gattgacaga
     6901 cgggagttcc atcatgaaaa agttagttct tgccgcatta cttgcttcct ttactttcgg
     6961 tgcttctgcc gcagagaaaa tcaattttgg cgtttcagcc acctatccac cctttgaatc
     7021 tataggtgct aataatgaga ttgtcggctt tgatatcgac ctggcaaaag ccttgtgcaa
     7081 acaaatgcag gcagaatgta cttttactaa tcacgcgttc gacagcctga tcccgtcact
     7141 gaaattcaga aaatatgacg ccgtaatctc cggtatggat atcactccag agcgtagcaa
     7201 acaggtatcg tttaccacgc cctactatga aaactcagcc gtcgtgattg ccaaaaaaga
     7261 tacctacaaa acgtttgccg atctgaaagg caaacgtatt gggatggaaa acggtactac
     7321 gcaccagaaa tatattcagg atcagcaccc ggaagtgaaa actgtctcct atgacagtta
     7381 tcagaatgcc tttatcgatc tgaaaaatgg ccgtattgat ggggtatttg gtgacacagc
     7441 ggtggttaat gaatggctga aaaccaaccc gcaactgggc gttgcgactg aaaaagtgac
     7501 cgatccgcaa tacttcggca ccggcctggg tatcgctgta cgtccggata acaaagctct
     7561 gctggaaaaa ctgaataacg cgctggcagc aattaaagct gacggtacat atcagaaaat
     7621 caatgaccag tggttcccgc agtaagattc agaaagccgc acctgcggct tttttattgc
     7681 ttaaccagca gtgtcagcac ttcgtaatgc gcagtgtgcg ggaacatatc aaaaagctgt
     7741 acccgttcga tgcagtaacc tggcagttcg cggatatctt tcgccatggt ttgggcgtta
     7801 cagctggagt agataataaa acgcggtgcc atcgttgaga gataatcaca cagcggttta
     7861 ccaatgccgc ggcgcggcgg gttaaccagc accagctccg gcacctcccc ctgagcagtg
     7921 gcaaactgag tggagtccag cgcctgaaat tgcaaacgcg ttaagcccag ttcagcggct
     7981 gactgctttg cacaggcaat ggcctctggc gcaatttcga tcccggttaa ctgcatgtca
     8041 ggcatcgcgc agtgtaaacc aaagcccccc acaccgcaga acagatccca catatgttta
     8101 accgacagct gtcgtaccca gtcgcgcgcg gtggcgtaca actggctggc gaccgccgga
     8161 ttagtctgga agaaactttg cggacggatc cacagcggta cgtcattaaa acgctctgcc
     8221 agcgcctgtt gttcagtcag gtaaatctcc gtctcccctt ccataatcgc catatgtacc
     8281 ggctgaatat tgacggtaat aactttcagc tgcggtagtt gttcctgtaa ccacggcagc
     8341 gccttacgca gttgcgccag cttggtatca gaacgcagta caaagcgcag catcatgccg
     8401 ccatcgctct ggctttcagt cagcagaatg tatttcagtt cgccacgctt acgcgccacg
     8461 ttgtagggcg ttaaccccgc tcgggcgata aacggtttta gcgccgcaaa aacgggcgca
     8521 aatgaggcag gataaagcgg acagtcacaa aggtcttccg gcgtgccgtc gcgatgcagc
     8581 ataccgagca gtggtttttc aacgctacca ctcaccacca ttttggcttt attacgaaac
     8641 ccttgttccg ggcctgacac cggcgcgcac cattcctcaa ccggaaagtc ggcgagcaga
     8701 tttttaagat cggcggtttt agcggagagt tgctctggaa tcggctgcgt tatccactga
     8761 caggaacgac agcgacccgc gtcgtaaagt gcgcactgca tacattgacc ttcacatcat
     8821 caggggcgac gattatacac attattgcaa ctggaagaac cgccgactgg tcgagggaac
     8881 gaacagcagc atcaggatga gcatatccgg cagcttctgc agcatcaggc tatggaagat
     8941 ttcacgtttt gattcaccgg gaatgctgaa cagctccgga taaccatacc ccagcgaagc
     9001 tgcccacaag taacttgcgg ccgtgatttg tgtcaacaga tacagccagc gcgcccagcg
     9061 acgccctttc accagtgaaa acgcacacca gatctcaatg aaaaccagca ccagactgct
     9121 caaaaagacc agcgttaaac tccaggtttg tacgctgcga tgaatgaact cgccaatacc
     9181 gcgcacgccc agcgtattga aaatcatcag cacgtcgagg ccacggatca taataatggc
     9241 gagcgccgcc acctgcacca gcgcaggcac attcaggcga gcatgagatg aagatgtttt
     9301 cttaaaaaat cccaacgttt cgtcttccat gaaaacaatg ccgcgacatg cgcggcatta
     9361 tgtagccagg ttggcaaatt ttagtgtctt cagccacgtc ttgcacgctg gatgtcgcgt
     9421 atccgctgtt tttctgcgcg cgccatcaga taccaggcga taaatccgac aattccgacc
     9481 gcgccgagga tcaatgttgc cagggcgttg atttccggat tcacccccat ccgcacgctg
     9541 gaaaagacca gcatcggtaa agtggtggct cccggcccgg aaacaaagct ggcgatcacc
     9601 agatcatcaa gcgacaaagt aaaagccagt aaccagccag aaatgatcgc gggcatgatc
     9661 atcggtagcg taatgacaaa aaacactttc agcggcgtcg caccgagatc catcgcggct
     9721 tcttctatcg agcgatccag ttcccgcaga cgcgacgaaa taacgactgc cacataagcc
     9781 gtacagaaag tgacatgcgc cagccagatg gtgagcatac cgcggtccgc aggccagcca
     9841 atagcatgag caagcgcgac gaataacaac aatagcgaca agcccgtgat gacatctggc
     9901 atcaccagcg gcgcggtgat cataaaggca aaaccatttg atccgcgaaa cctaccaaag
     9961 cgcaccagca cgaccgccgc aatcgtcccg aggatcgccg ccgccgtcgc cgcacaggcc
    10021 gcaattgtca ggcttaaacc aaccgcactc atcatcgcgt tatcgcgcaa taactcacca
    10081 taccagcgtg ttgaccagcc ggcccacacc gtcaccagtt tcgagctgtt gaatgaatag
    10141 atcaccagca tcagcattgg cgcgtagaga aaggtgaagc ccagcagcaa aatcacaatc
    10201 cgccagggcg aacgaacaac cggtaaatta ttcatccgtg ttctcccacg cttttttgct
    10261 ggtgtttgtg aaaccacatt atcggcacaa ttagcagcag caacatgatg atcgctaccg
    10321 ccgaggccac cggccagtcg cggttattga aaaactcctg ccacagcacg cgcccgatca
    10381 tgatgctgtc cgggccaccg agcagttcag ggatcacaaa ctcgcccaca gccgggataa
    10441 acaccagcat cgatccggca ataatcccgc ctttggtcag cggcacgatc acagtaaaga
    10501 acgttttcag cggtcgtgca ccgagatcca gtgccgcttc caccagcgaa taatcaatac
    10561 gaatcaaggc ggtgtagatc ggcagcacca taaacggcac ataggcgtaa acaatgccga
    10621 tataaaccgc caggttggta tgcagaatgg tcaacggttg atcgataacc cccagccaca
    10681 gcagaaaatt attcagcaca ccgttgtttt ttaatattcc catccaggca taaacgcgga
    10741 tcagaaacga ggtccacgac ggcaggatca ccagtagtaa taagatatta cgggtcgaag
    10801 gcttactgtg cgccaccgcc cacgccagcg gatagccgat cagtaaacag caaaatgtcg
    10861 aaatcgcagc cacctgtagc gactggagat aagcatcgaa atagagcgga tcgtcagtca
    10921 gttgcagaaa attaccgaga ttaagagtga tggacagttg cccgtcagcc cactccatca
    10981 gttcggtata aggcggaatg gcgcgcgcca tctccgccag acttatttta aagacgatca
    11041 gaaatggcag cagaaacagc aggatcaacc agatatacgg caacgcaatg accagtttgc
    11101 gcccatgctt catttgcagc tgcgacaacc acagcttaaa tccgcccggc ggtttgggct
    11161 gggcagcagg ttcaagtgta ctcatctttg ctccttaaac cgtcagcacc acacagctgt
    11221 ccacttccca gcacaaacgc acttcgtcgc cccaggttgg taaccctttg cgatatcgat
    11281 gagcgttttg tagctgagcg ctgatcatct gtccgctctt gagacgaacg tgatacaccg
    11341 aaagatcgcc gagataggca atgtgcatca cctcacctac cgcaaagtta aaaccgtcag
    11401 cgggaggttc ctcgcaaagc atgatttttt ccgggcgcag cgccacatgc accggcacat
    11461 tatcgaccac cgaagcatct acatcgactt tcagtggatg caccagcccc ggcgaataaa
    11521 gcaccagacc atcttcctga cgctctttga gtacaccttc aaagacatta accgagccaa
    11581 taaattcggc gctgtagcga gtggtcggat gctcgtagat ctcttccggt tcgccaatct
    11641 ggacaaattt ccctcgattc ataatggcga tacgccccgc catggtcatc gcctcttcct
    11701 gatcgtgggt gaccatcaca caagtcacac cgacacgctc cagaatatcc accacttcaa
    11761 gctgcatccg gtcacgcagc tttttatcca gcgcgcccat aggctcatcg agcagtaata
    11821 gtttcgggcg cttggcaagg cttcgggcca gtgccacacg ctgtcgctga ccgccggaaa
    11881 gctgatgcgg tttgcgtttg gcgaactcct gcatatgcac cagcccgagc atctcattga
    11941 cccggctggc aatttccgct ttcggtagtt tgtcctgttt caggccaaaa gcgatgttct
    12001 gttccacggt catatgcgga aacagcgcgt aagactgaaa catcatattt atggggcgca
    12061 ggtaaggcgg aacctgtgac aaatcgacgc catcaagcat tatctgtccg gcagaaggtt
    12121 gttcgaaacc tgccagcata cgcagcagcg tggacttgcc acagccggat gcgcccagca
    12181 gcgcgaagat ttcgccttta tagatggtca ggctgacatc atcgaccgca tgctgaccat
    12241 cgtaagattt agtcagatta cggatttcta atagcggcgt cagcacctta cgggttttcg
    12301 cctgcgggcg agggatagcg tcattcacgg ctgctctccg gcattgaatg tgtgcaagcg
    12361 caccataatg gtgcgaattg ccggcgggcg tggtgcgccc gtccggcatc tacgactgcg
    12421 gattattttc cgctcttcac tttggtccac gcgcgggtgc gcacacggtc gattttcgga
    12481 tcctgtactt tcagagtgaa cagcttcgca cgaacatccg caggcgggta aatacccggg
    12541 ttatcacgga cttccgcact caccagcggc gtagctgctt tattggcgtt agcatagaac
    12601 acatggtcgg aaatatgcgc caccacatcc gggcgcagca ggtaattcag gaactgatag
    12661 gcttcgtctt tatttttggc atccgcaggc atggcgaaga catcaaagaa cgccatcgcc
    12721 ccttctttcg gaatcgagaa cgagacatta acgccattct tcgcttcctt cgcgcggttt
    12781 gacgcctgcc agacatcgcc cgcccagcca atagcgacgc agatatcgcc gtttgccagg
    12841 tcgttaatgt attgagatga atggaaataa cgaatgttcg ggcgcagctt taacagcaga
    12901 tcggttgccg gtccggtgta atcatctgct ttagtgctgt tgggatcttt accgagataa
    12961 ttcagcacgg tagcaaacac ttcttccggt gcatcaagga aagagacgcc gcagcttttc
    13021 agtttttcca gattttcagg cttgaggatc aagtcccagc tatcgacggg cgcgttttcg
    13081 cccagcaccg ctttaacttt atcaacgtta tagccaatcc cggtcgtcgc ccacatatag
    13141 ggcatagcaa atttattgtc gggatcgtgt ttggcaacta acttcagcag ctccggatcg
    13201 agattcttcc actccggcaa tttgcttttg tccagcggct ggaaaactcc cgcagtcaac
    13261 tggcgttcaa gaaagcttgc tgacggcacc accaggtcaa agccggtgct acccgccatt
    13321 aatttgcctt ccagtacttc gttagagtcg aaaacatcgt agacgacttt aataccggtt
    13381 tctttttcaa aattggccac cgtgtccggg gcgatataat cagaccagtt ataaatgtga
    13441 agtgtttttt gttcagccgc gagcgtgccg acagagacgg ccatcagagc acccgtaacc
    13501 agacccgata gccatttttt atttaaggcg gtcatatctc tttccttctg aaagttcgtt
    13561 aacaaacgaa caaaagttgt cacactgaaa gatatgcaat aaacgtgcat attttttatc
    13621 ggcgcggtgt aagaaaagag aactctctcc cggccagcac tgctcgctat ataaaagcat
    13681 cgcaagaata actttagcca gggtttgaga ctatagctcg gattaaaaaa cggtatttaa
    13741 caaattttta tgcgtatttg ctctatgtga taaagccaaa tcggcacaag agaagtgaaa
    13801 taatctttaa gaaaaggtta agaatagcag ataattttgc agcacgcagt cactgcggca
    13861 gcgactgcgt actcgaaagg agttatcagt ggaggaaata gttttcggta acgttgtttt
    13921 gcccttcatc gtcagtcggt aacagcagtt gatgggcatt agcttcaaga atgaccatcg
    13981 aaatctgctc ttcgctctga cgcacgaacc atgcaaactg ctcataggtt acgccctgca
    14041 taacggataa agactggcaa accaccagct ttggcagatt atcatcctgc atatcaagaa
    14101 atgctttcac ggtcagcgaa ctggcattga tggcagataa atccgccgcc agcggcaata
    14161 cggctgaagg tcggacttcc gccatggcag aaaacaggat cgtgttatcg atcagatcga
    14221 ttttggcatc aaagacaccg tcgaaattct gcatatgggg cagatgcaga gcctgacagt
    14281 tatcacattc aaaaaaactc atccccaggt catcgagcca ttgacgcagc gtatccagac
    14341 caggaacgac cagcgatgtc ataattttgt acaacctctt tcgataaaaa gaccggcaca
    14401 gcttacgcaa aaagcgcagg caaaaccatg atcagtaatg tgattgcgac taaccacccg
    14461 ttttcaggca atattccgtc gtagcgtggc gttcgatcca gcggatcatt ttacccgcga
    14521 tgtcgatacc ggtggttttt tctattcctt ccagccccgg cgacgcattc acctccatca
    14581 ccaatggccc gcgattagca cgcagaatat ccacaccagc aacgtccagc gccatcgttc
    14641 gcgcggcttt tatcgcgatt tcacgctcct gtggtgtgat actggcgacg cttgccgcgc
    14701 cgccacgatg caaattggaa cgaaaatcgc cctctttcgc ccgccgttca atcgcagcga
    14761 ccacttcatc gccaacaacc agacagcgga tatcgcaccc ttgcgcctct ttgatatatt
    14821 cctgcaccag aatatgcgcg ttcagaccgc ggaaagcgtc aatcacgctt tccgccgcct
    14881 ggcgcgtctc cgccagcacg acaccaattc cctgcgtgcc ttcaaccagc ttgaccacca
    14941 gcggcgcacc accgaccatg tcgattaaat cgctggtatc atccggcgaa tgcgcaatgc
    15001 ccgtgacagg caggtcgatg ccctgacgcg ccagcagttg catggaacgc aatttgtcac
    15061 gcgcccgggc aatggcgacc gactcattga gcggatagct ccccagcatc tcgaactggc
    15121 gcagtgccgc cgtcccataa aaggtaatgg cggtaccaat acgcgggatc acagcatcaa
    15181 aatggggtaa cttgcggcct ttgtagtgaa tagaagacgc cgcaggattt atgttcatgt
    15241 agcaagaaag cggatcaaga atttcaacca ggtgaccgcg ctgtatagcg gcttcacgca
    15301 gccgcttaca cgaatagagc gttccatccc gggacaatat ggcaattttc accctgcacc
    15361 tctctgtcag acctggtcaa aagcccgcgt atcatacatc ggcgtggcgg ttttagcgcg
    15421 tcgcccaacc ctgtttgtgc aaataatcca gtataaatgg gcggctttct ttaatgattg
    15481 ttcggcggat atgatcgctc caggtatccc ggcgattatt gctgccacgg gtgaggtaat
    15541 attccgccag ttgttcgtca tactgcgcca gtgcgtcttt atccagcggt tgatagttgt
    15601 tttcatgcac caaaatggag gccggtaaac gcggcttaag atccggatta tccgcaggcc
    15661 agccaaggca cagcccaaac agcggcagaa catgctgcgg taatttcagc agctccgtca
    15721 ccgcttcaat attattgcgc aggccgccga tatatacccc gcccaatccc agcgattccg
    15781 ctgcggttaa tgcattctgc gccatcattg ccgtatcaac gacaccgagc aacagttgtt
    15841 ccgccaggcc aagttgtgca tccggacaga tctgtaaatg gcggttaaag tcggcacaga
    15901 acacccagaa ctccgccgct tgcgctacgt gtttttgccc gccggtcagc gtcaccagtt
    15961 catcacgtaa cgctttgtcg gtaatgcgaa taatgctact gcactgcaaa aaactggaac
    16021 tggacgtcgc acgggcgctg ttaataatcg cctcacgctg cgcttcggaa atgggttcat
    16081 cagtgaaatg gcgaatggag cgatggccac aaataagttc aatggttggc gtcattatct
    16141 ttttctcttt ctgaacgtga atattgcggt ggacggttca tcagctgtgg ggcaagacgt
    16201 tttgccacct gaagaataac caccaccgca gcgggaagca tgagcaaaac accgagaaaa
    16261 atcatcagaa tctgcacttc tggccgagaa aatggctcag gcagcgacag ggagccgctt
    16321 accgacagca gcgccaccgc cagcaacatc attccgataa attccagtat caacacgctt
    16381 ttaggcaatt taccgatcgc gcgcatacgc ttccctctgc aaagtgagcc ttcagtctaa
    16441 aacttttcac tgtattgtgt ttaacagtta tagcttttag caattaatgc aacaggttaa
    16501 acctactttc agcgaataca ttttagcgtg atcattacag gtataaatct atgaggagag
    16561 aaataatgca aaccgttatt tttggtcgtc cgggttgccc ttactgtgtg cgtgcaaaag
    16621 atctggctga gaaattgagc aatgaacgcg atgatttcca gtatcagtat gtagatattc
    16681 gtgcggaagg gatcactaaa gaagatctac aacaaaaggc aggaaaaccc gtagaaaccg
    16741 tgccgcagat ttttgtcgat cagcaacata tcggcggcta taccgatttt gctgcatggg
    16801 tgaaagagaa tctggacgcc tgatcgtctg acaagccctc gcgttgaggg ctttattgat
    16861 tttttctgtg ctgtggttta aacaaactac tgataaataa gaaacacaat gcccccagcg
    16921 cacaccagaa caccgcactt aataaccatg ccagctcttg ccagaatgag cgcgtcggtg
    16981 aaaaaaacag ccgcataatg agcatcgaac agggtgccgc cagcattgcg ccaaacagag
    17041 gtttcaggac ttctctacga tgtgaaaaga agctggcaac tgcaccagga agaatgaaaa
    17101 atagcaaacc gatttcagga tgcccggcag cccgaaaagc gcctttcatg tgcgtcgcca
    17161 gaaaaagaca caccacaatg aagaggacaa aacagcagat tgcccccgcc caacgttgtt
    17221 tatgtttcac tcgttcctcc tgacactgcg tctatcgaac acatttttcg ccagtgtggc
    17281 gttcagtaag ataaagccgc ttcgcattcc atgctaatat aggccaacgc aattcatata
    17341 gccgttgata cctaatgtga ttacactagt aaaatatatt gttactttac tatcgtttag
    17401 gtgcgctgaa tgaatctgcg ccctgaattc tggtaaaaaa cattatcgta aattaccatt
    17461 tctttcaaca gcttactagt aaacaagaag ttagcctccg tgaatataaa cgtcgccgaa
    17521 ttgttaaatg ggaattacat tctgttatta tttgtggtcc tcgcgcttgg gctatgtctc
    17581 gggaaattac gacttggttc gatccaactg ggtaattcca ttggcgtttt agtcgtatcg
    17641 ctgttattag gccaacaaca tttcagcatt aacaccgacg cgcttaatct tggctttatg
    17701 ctgtttattt tctgcgtcgg ggtcgaagcc ggaccgaact ttttttccat tttttttcgc
    17761 gatgggaaaa attacctaat gttagcactg gtgatggttg gcagtgcgct ggtcatcgcc
    17821 ttagggttag gtaagctgtt tggctgggat attggcctga cggccggtat gttagcaggc
    17881 tctatgacgt caacaccggt tctggtcggt gctggcgata cactgcgtca ttccggcatg
    17941 gaaagcaggc agctctcatt ggcactggat aatctgagcc tcgggtatgc cttaacctat
    18001 ttaatcggcc tggtgagttt gattgttggt gcgcgttact tgccgaaatt gcagcatcag
    18061 gacttacaga ccagcgccca gcaaatcgcc cgcgaacgtg gcctggacac agatgccaac
    18121 cgtaaggttt atttgccggt gatccgcgcc taccgcgtcg gcccggagct ggtagcctgg
    18181 accgacggca aaaatctgcg tgaactgggt atttatcgac aaactggctg ttacattgaa
    18241 cgtattcggc gtaacgggat tctggcaaat ccagacggtg atgccgtact acaaatgggc
    18301 gatgaaatag cgttggtagg ctatcctgac gcccacgccc gactcgatcc cagtttccgt
    18361 aatggcaaag aagttttcga tcgtgacctt ctcgacatgc gtatcgtcac tgaagaagtg
    18421 gtcgttaaaa accataacgc tgttggcaaa cgcctggcac aactgaagtt gaccgatcac
    18481 ggttgcttcc ttaaccgcgt catacgtagc cagattgaga tgccgattga tgacaacgtc
    18541 gtgcttaaca aaggtgacgt tttacaagtc agcggcgatg cccgccgggt aaaaaccatc
    18601 gccgatcgca tcggctttat ctcgattcac agccaggtca ctgacttgct ggcattctgc
    18661 gccttctttg ttattgggct gatgatcggg atgatcacct tccagttcag cacattcagt
    18721 ttcggtatgg ggaacgctgc cgggttgtta ttcgccggaa ttatgctggg ctttatgcgt
    18781 gctaaccacc cgaccttcgg ttacattccg cagggtgcat taagcatggt gaaggagttc
    18841 ggcttgatgg tgtttatggc aggcgttggt ctgagcgctg gtagcggtat taataacggc
    18901 ctgggcgcga ttggcggtca gatgttgatt gccggattga ttgtcagtct ggtgccagtg
    18961 gttatctgct tcttgttcgg tgcttatgta ttgcgaatga accgcgcact gttgttcggc
    19021 gcaatgatgg gcgcacgcac ctgcgcgccg gcaatggaga tcatcagtga tacagctcgc
    19081 agtaatatcc cggcactggg ctatgcgggc acttacgcaa tcgccaacgt cctgctgacg
    19141 ctggcaggga caatcatcgt catggtatgg ccaggattag gataaaactg aagttgccct
    19201 gaaaatgaaa ttttttttgc acaaccgcag aacttttccg cagggcatca gtcttaatta
    19261 gtgccactgc ttttctttga tgtccccatt ttgtggagcc catcaacccc gccatttcgg
    19321 ttcaaggttg atgggttttt tgttgtctaa aatttatcta ctaccctgca accctctcaa
    19381 ccatcctcaa aatctcctcg cgcgatagcg gcttacggtc ggtgacaaaa tgcagcgtca
    19441 tctcctcaat aaacgcatca agcgcgcggg cggttccggg ttcaaaccat tgttcgagcg
    19501 tttgctgact gcgctgcatc cagttttgca ttaccgtttt taatagcggt tttcggctag
    19561 ccagcgcgta gagctggtac atcagctcca tgttatccgg cgttgcaacc tgtgagctgt
    19621 aaatcatatc ggtgatagcc tggcatgcgc ccggagcatc actaacatcg ctaaaaaatg
    19681 cctgatattg ccgggacatg atctcagtaa aactgctgaa cgcctccagc aacaactcat
    19741 caattcctga aaaatagtag gtcatcgacc ccaacggtac cccggcaagg gtagcaattt
    19801 tgcggtgcgt aacagcatgt attccataaa gtttcaccgc ctccagcgtg gcctggataa
    19861 ttttttctcg ccgttgcgga tcgttagcgc gacgcatgtc atttcctctc tctgcaattg
    19921 tgtacaaatg tacacaacct tgctaatgtt gtcttccctc ttcttatttt gacgtgatct
    19981 atgaccgtaa attcttcacg taatgcattg aaacgccgaa cctgggcgct gtttatgttc
    20041 ttctttttgc caggcctgtt aatggcgtcc tgggcaaccc gtacgcctgc tatccgcgat
    20101 attctttctg tttcgatcgc tgaaatgggc ggagtcctct ttggtctgtc gatcggttca
    20161 atgagcggta ttctctgctc ggcgtggtta gtgaaacgct ttgggacgcg taatgtcatc
    20221 ctggtcacga tgtcctgcgc attgatcggg atgatgatac taagtctggc actctggctg
    20281 acatcgcccc tactcttcgc ggttggtctc ggcgtctttg gggcaagttt tggttctgcg
    20341 gaagtggcga taaacgttga aggtgctgcc gttgagcgag aaatgaataa gacggttttg
    20401 ccgatgatgc acggttttta tagcctgggc acgctggcag gcgctggtgt cgggatggca
    20461 ctaacggcct ttggcgttcc ggcaacggtg cacattttat tggcggcgct ggtaggcatc
    20521 gcgcctattt atatcgccat tcaggcaatc cctgatggta cgggcaaaaa tgctgccgat
    20581 ggcacccagc atggtgaaaa aggcgtacct ttttatcgcg atatccagtt gctgctgatt
    20641 ggtgttgtgg tgctggcgat ggcctttgcc gaaggttctg ccaacgactg gttaccctta
    20701 ttaatggttg acggtcacgg ttttagccct acttccggct cgctgattta tgtcggtttt
    20761 accctgggga tgaccgttgg acgctttacc ggcggttggt tcattgaccg ttacagtcgc
    20821 gttgccgtgg ttcgggccag tgcactaatg ggggcgttgg gtattgggct gattattttt
    20881 gtcgatagcg cctgggttgc tggggtgtct gttgtacttt ggggactggg tgcctcgttg
    20941 ggcttcccgc tgaccatttc tgccgccagc gataccggcc ccgatgcacc aacccgcgtc
    21001 agcgtggtag ctacgaccgg ttatctggct ttcctcgtcg ggccgccact gctgggctat
    21061 ctcggcgaac attatggatt acgtagtgca atgctggttg tactggcgct ggttattctc
    21121 gcggctattg tcgcgaaagc cgtcgccaaa cccgatacca aaacgcagac ggcgatggag
    21181 aatagttgat gagcattaaa ttaattgcgg tagacatgga tggtactttc ttaagcgatc
    21241 aaaaaaccta taaccgtgag cggtttatgg ctcagtatca gcaaatgaaa gcacaaggca
    21301 ttcgctttgt ggtcgccagc gggaatcaat attatcagtt gatctctttc tttccggaaa
    21361 ttgctaatga aatagccttt gtggctgaaa acggcggctg ggtagtgagc gaaggcaaag
    21421 atgtttttaa tggcgagctg tcgaaggatg cgttttctac tgtcgtggaa catttgctga
    21481 cgcgcccgga agtggaaatt attgcctgcg gaaaaaatag cgcctataca ctcaaaaaat
    21541 atgacgatgc catgaaaacg gtggcggaaa tgtattatca ccgtctggaa tacgtcgata
    21601 actttgacaa cttagaagat atcttcttta agtttggtct gaatctttcc gatgaactga
    21661 ttccacaagt acaaaaagca ttacatgagg ccatcggcga tattatggtg ccggtccaca
    21721 ccggcaacgg cagcatcgat ctgattatcc ccggcgtaca taaagccaat ggccttcgcc
    21781 aactgcagaa attatgggga atagacgaca gcgaagtggt ggtctttggc gatggcggta
    21841 acgatattga gatgctgcgt caggcaggct ttagttttgc aatggaaaat gccggcagcg
    21901 cggtcgtcgc agcggcaaaa taccgggcag gctccaataa ccgtgaaggc gtactggatg
    21961 tgatcgataa agtgcttaaa cacgaagcgc catttaacca ataaaacggc agcacgcccg
    22021 gcaacacaat acctgaatga aggctgcata attcgcggaa attaacccgc tgcattattt
    22081 caggcgaaat gattatgaaa aattgtctac tgttgggcgc gcttttaatg ggctttactg
    22141 gtgtggcgat ggcgcaaagt gtcaccgtcg atgtgccgag tggatacaaa gtggttgtgg
    22201 ttcctgattc agtcagcgtt ccacaagcgg tcagcgtcgc gactgtgccg caaaccgtct
    22261 atgtggctcc tgcgccagct ccggcttacc gcccgcatcc ttatgttcgc catcttgcca
    22321 gcgtaggtga agggatggtt atcgaacacc agattgacga ccatcaccat taattcgaga
    22381 atgcctgatc gcacaaagca atcaggcatt tttttaccct tcgtgcgaat ttcccatctg
    22441 tttatctttt aaaaagataa ccatcagcaa cagccacaaa attccgttga caaggttgaa
    22501 gagattaaac agtccgttgc ccccgttcag ccagacatgt ttgctgattt caataccgac
    22561 ggtaaagatc agcatttgca gcatccccat cgccgcagaa accgtacctt tactcatatc
    22621 gctggcaaac agggttaatc gcaccagtcc cgcattcgcc agaccaatac cgaaagcata
    22681 aatacttaac ccggcggtca tccataaata cgcgtgcgat gagataaccg ttgccgcagc
    22741 agcgaccaac agaccaatca taatcggcca gccgcccata ataatcagcg aacgtacggt
    22801 gcggcgcgag gtcagacgcg ctaacagcaa gttacccgca attaacgccc cgaaaatagg
    22861 cacttgcagc aagccatatt catagctgct caactgctca ccggtaataa tgataatcgg
    22921 cgactgggcg atccacgcca acaacggcag gctaacgaat cccagcgcca gcgcccccgc
    22981 cacaaagcgg ccgttcttca gcaccagctt atagtcacga ccgagttctt tcagcgacag
    23041 tttctcgcct atacgcgtgg cggtttcagg catggctcgt tgcagaccga aaaaggagat
    23101 cgctgccaat gcggcaaaca agacaaacat cccttcccag ggcagcacat ggatccacgc
    23161 cgcgcccacc agcggaccaa gtagtggagc aatcagcgcc acgttcgcca ttagcgcggt
    23221 gatcttgata caaaccgcct cttcgaagga ttcctgaatt gcggcgtatc ccacagcgcc
    23281 aatgaaacag aggcttatgc cctgcaagaa gcgcaacagg gtgaattgtt caatgttttg
    23341 cgccagtaat attgccagac aggtgacgat aaaccacacc actcccgcca gcatcaccgg
    23401 acggcggcca atacgatccg acaacggccc caggagccat tgtaaaaaca tcccgcccgc
    23461 cagatacgca gtcatcgaag taggaaccca atcaatgccc gcctgatatt gttccaccac
    23521 ggccaacata ccgggttgaa tcatatcgtt gccgatatag gttgaaaatt cgtaaagcac
    23581 cagacagaga gggaaaagta acgcctgacg tccaagcctg gcaccggaag ctaatttatt
    23641 ttgcatgcaa tttcttcgcc aataataatc gcgcaaagtt taataaaagc gcagctaacg
    23701 agaaagcgaa ttttgtagct gaaaccacgg ttaagcacat tcttacatta ttgcgagtat
    23761 agctacgctt tctttaagtt ttatttaacc tttgcccgtt acaatcaccc accgtaaaca
    23821 ggccgcttga gggaaataag acgatgccgc tttacccagt ttaacctgca ctttattctc
    23881 aacgacttgc ctgtattggc tcccttttaa tcactttgcg tcgggaagtt atgctggaaa
    23941 atttgaatct ctctctattc tctcttatta acgcgacgcc agactctgcg ccgtggatga
    24001 tctcgttggc gatttttatt gcaaaagatt tgattaccgt ggtgccgttg ctggccgcgg
    24061 tactttggct gtgggggctt acagcacaac ggcaactggt gataaaaatc gctatcgcgc
    24121 tggcggtcag cctgtttgtg tcctggtcga tggggcatct ttttccgcac gaccgaccct
    24181 ttgtcgaaaa tatcggctat aacttcctgc atcatgcggc ggatgactca ttcccgagcg
    24241 atcacggtac ggtgattttc acctttgcac tggcattttt atgctggcat cgcctgtggt
    24301 ccggctcact tttaatggtg ctggccgtcg tcattgcctg gtcgcgcgtt tatcttggcg
    24361 tccactggcc gctggatatg ctcggtggat tgctggcagg tatgattggc tgccttagcg
    24421 cccagattat ctggcaagcg atggggcata aactctatca acgtctgcaa tcgtggtatc
    24481 gcttctgttt tgcattaccg atccgcaaag gctgggtgcg tgactgatct ctgctaaaaa
    24541 gtgtagtatt gagcggctcg cttcaataac tattcagagg gattatggaa acacgtcgcg
    24601 aagagcgtat cgggcagctg ctgcaagaat taaaacgcag tgataagtta catcttaaag
    24661 acgccgccgc cctgcttggg gtttcggaga tgacgattcg tcgcgatctg aacaaccaca
    24721 gtgcgcccgt cgttttgctc ggcggctata ttgttctgga accacgcagc gccagccatt
    24781 acctgttaag cgatcaaaaa tcccgcctgg tggaagaaaa acgccgggcg gcaaaactgg
    24841 ctgcgacgct ggtagaaccc gatcagaccc tcttttttga ctgtggcacc accacgccgt
    24901 ggattattga agcaattgat aatgaaatcc cttttaccgc cgtttgttat tcgctaaata
    24961 cctttctggc gctgaaagag aaaccccatt gccgcgcgtt tctttgcggt ggtgaatttc
    25021 acgccagcaa cgccattttc aaacccatcg attttcagca aacgctgaat aatttttgcc
    25081 cggatatcgc tttttattcc gctgcgggcg tgcatgtcag taaaggcgct acctgcttta
    25141 atcttgaaga gttgccggta aaacactggg ccatgtcgat ggcgcaaaag catgtgctgg
    25201 ttgtcgacca cagtaaattt ggcaaggtgc gtccggcgcg catgggtgac ctgaaacgct
    25261 ttgatattgt tgtgagcgat tgttgcccgg aagatgagta tgtgaagtac gcgcagacgc
    25321 agcgtattaa gttgatgtat taatgacgta taaccggatg acgtttcgcg ccatccggtt
    25381 atcagaagat taagagaacc agctgccgaa ccactgatgg aatttcatca tcacgaaatc
    25441 ccacatccga ccaaagaatc cgccctcttc cacattttcc atcacgatca gcggacgctg
    25501 ctcaatggat ttaccgttaa gctggaaatc aatggtgccg acaacctgac cttttttcag
    25561 cggtgcggta agctgcggtt ccgttaacgt ataactcgcc ttcaggtttt tcagctgccc
    25621 acgcggtatg gtcactgagc ccgcttcgcc tgccccgaga ttcacttcgc tcttatcacc
    25681 aaaccagacg cgctgagtca caaatgtggc atcaggttta attggcgtca cggtttcaaa
    25741 gaaacggaag ccccaggtca ataatttctc agactcatta aaacggatac ggtcagtttt
    25801 cgcccccagc actacggaga ttaaacgcat atcgccctgg gtagccgaag caaccagatt
    25861 atatcccgcg cctgccgtgg ttcctgtctt catgccatca acattcagat tgctgctcca
    25921 cagcagacga ttacggttag gctgacgaat tttgttgaag gtgaactctt tctctttatg
    25981 aatggcgtac tcttccggca catcgtggat caatgcttta cccagcaatg ccatatcgcg
    26041 cgcggtactg aactgccccg gcgcatccag accgtgcacc gtctggaagg tagtgttggt
    26101 cagacccagt tttttggcat aaccattcat cagaccaata aatgactcct ggctcccggc
    26161 aacgtaatca gccagcgcaa tacaggcgtc attaccggac tggataatca cacctttgtt
    26221 caagtctgcc accgaaacct gatcgcccgg tttgaggaac attaccgatg aaccacgcag
    26281 tgccggattt cccgtcgccc aggcatcttt accgaccgtc accatatcgg tgagtttaat
    26341 cttatcggcc ttaagcgcct gcccaaccac atagctggtc atgattttag tcaggctcgc
    26401 ggggtccagt ttttcatccg cattgccttc tgccagcact ttaccgctgg cgtaatccat
    26461 taaaatccat gcacgcgcat ccacgctcgg cgcttcaacg gtttgttccg ccgcgaatgc
    26521 cgttggggca aaaaggaata aaaatgcaga acccgctgca agaccacgaa ggagagagga
    26581 gtattgcgtc ataaatgcca cccgagtatc cattcaaaaa aaatacgtta tatcgccgta
    26641 tgataagttc ggtgattaat aacgcacaca caaacttaaa gaaaccgtaa gttggtaaag
    26701 tttttaaagt ttatgcaata tcctgccgct tcgctgcttc tggtgtcatt tttttgccag
    26761 ctgttgctta aaagttaact ttacttcacc atcggagtcc tgttcagcct gtggctgcct
    26821 gtcggaaaag gggtgagtat gattacgctg tggggtcgga ataattcaac caacgtaaaa
    26881 aaagtattgc tgacgctcga agaactggaa ctaccttatg agcaaattct cgcgggccgt
    26941 gagtttggga taaatcacga tgctgatttt ctggcgatga accctaacgg gctggtgccg
    27001 ttgttgcgtg acgacgaaag tgatctcatt ctttgggaat caaacgccat tgtccgctat
    27061 ctggcggcac agtacgggca aaaacgcctg tggatcgact caccggcacg tcgtgcggaa
    27121 gccgaaaaat ggatggactg ggcaaaccag acgctcagta atgctcatcg cgggatcctg
    27181 atgggattag tcagaacacc accggaagag cgcgatcagg ccgccattga tgccagttgc
    27241 aaagagtgcg acgccctgtt tgccctgctc gatgcggaac tggcaaaagt aaaatggttc
    27301 tcaggcgacg agtttggtgt gggcgatatc gctatcgcac cgttcatcta caatttgttt
    27361 aacgttggcc tgacctggac accgcgtcca aatctgcaac gctggtatca gcaactcact
    27421 gaacgccccg cagtgcgcaa agtggtgatg attcccgtta gctaattgcg tgggctaact
    27481 ttaagtaatt ccccactgga ctcgtcggtg agaacgtata aataaccgtc gggtccagtg
    27541 cgaacatcac gaattcgctg ccctctgtcc gttaaaatac ggccatcttc tgtcactttg
    27601 tcgccgttga cgctcatcac aatgacatct ttatctttca gcgcgccaat aaataatttt
    27661 tgctgccact gggggaattt gtcgctgtta tagaaggcca tgccgctcac ggcgggcgaa
    27721 tctttccagt aaaaaacagg ttgctcggtc ccggcgacga tctccccttt cgcctccggt
    27781 atcttaaagc ctgaatagtt gattccccag gttgccagcg gccagccgta gtttttgcct
    27841 ttttgcggga tattaatttc atcgccaccg cgcgggccat gttcattcag ccatagtgca
    27901 ttactccacg gattcatcgc cattccttgc ggattacgaa tgccataaga ccagatctcg
    27961 gcgcgtgcat cggattcctt tataaaagga ttatcatccg ggatttctcc caggtcggtc
    28021 agtcgcacca gtttgccctg taatttatcc agatcctgcg ccgtcgggcg ctgattgttt
    28081 tcgcccagag caataaaaag ataaccttta ccgtcaaata ccatccgccc gccaaaatgg
    28141 ttgccggtag acagttttgg catctgacga aagacggtgc ggaagtcggt cacttttgag
    28201 agatcatcac ttaagcggcc ataacccaca gcagttccgg ctttgccatc atcgccaact
    28261 tcggaataac ttaaccagat gcggcgcgac tgagcaaaat cgggcgctaa aaccacgtcc
    28321 agcaggccgc cctgcccgtg cgcccaaacg tccggcactc cggaaagcgg cgcagataat
    28381 ccttttcctg cttgccagtg acgcaactcg ccgccgcgca gagtgattaa cataccgtga
    28441 ttgtcgggta aaaaggccag cgcccaggga tggtcgagtt tgtcctgcag tacttcgaca
    28501 tttaccgttg caggagccgc ccagagagcg gaagaaagac aaataagggg cacaaggaaa
    28561 aaggattgtc gatgcataac gcctccggat tgactctggc ttaaagcgta gtcagtggag
    28621 gagataacaa atttattttt acaaaaactt aaacatgaag ggggagacgc tttctccccc
    28681 ttagttttca ggccttctca agcatggcgt gcttctgcag gctctggata ctcagcgtta
    28741 agctcatcag acaattttca agcttatcgg cgttgacggt aataacagtc gggcaatcat
    28801 ggtgcccact catcaaacat actgcggctg tcgctaatgc ttcttcagca tgatgaagag
    28861 cactccactc ttcctgatcc agatgaagat tcaaccgcag cgatttatcg tgcagttcgc
    28921 gattcagttt aaaaaagtta tctcgtagat gattgctttc gctgacggac atgtatcctt
    28981 ttgccttcct cagttgcacg tatgcggcga ggtcaacgcg tgcgtcgatc aaccggttca
    29041 gcagatcgat tcgctgtctg tcaacgaaca tacttcgatc ctcctctttc cagccttgtt
    29101 gcaattaaat agtatggcta ttttttgagc aaaatgctgt ctaggatcaa ttcttcacgc
    29161 cgaattaaca aaatttacat ttccttcgcc tccgccagcg aatgagattg cgatacaaat
    29221 tttatccaga actctgtaga atccctgccc tgacctttac tgtaactgaa tattttttaa
    29281 gctatgagca aagtaactcc ccagccgaaa atcggctttg tttcccttgg ctgcccgaaa
    29341 aaccttgtcg attcagagcg tattctcacc gaactacgca ctgaaggtta tgacgtggta
    29401 ccgagctatg acgatgcgga catggtgatc gtcaacacct gcggctttat tgacagcgcg
    29461 gtacaagaat cactggaagc cattggtgaa gcgttgaatg aaaacggcaa ggtaattgtg
    29521 accggttgtc tgggggcaaa agaagatcag atccgcgaag tccacccgaa agtgctggaa
    29581 atcaccgggc ctcatagcta tgagcaggtt ctggagcacg ttcatcacta cgtgccaaaa
    29641 ccgaaacaca acccattcct gagcctggtg ccagaacaag gtgtgaaact gacgccgcgt
    29701 cattatgcct atctgaaaat ttctgaaggc tgtaatcacc gctgcacctt ctgcattatt
    29761 ccgtctatgc gcggcgacct ggtgagccgt ccgattggcg aagtgttaag tgaagcgaaa
    29821 cgtctggttg atgcgggcgt taaagagatt ctggtgatct cgcaggatac ttccgcctat
    29881 ggcgtcgatg ttaaacatcg tactggcttc cacaacggcg agccggtaaa aaccagcatg
    29941 gtcagcctgt gcgaacagtt atcgaaactg gggatctgga cacgtctgca ctacgtttac
    30001 ccttatccgc atgtggacga cgtcatccca ctgatggcag aaggcaaaat cctgccctat
    30061 ctggacattc cgttgcagca cgccagcccg cgcattctca aactgatgaa gcgtccgggt
    30121 tctgtagatc gccaactcgc gcgcatcaaa cagtggcgcg aaatctgccc ggaactgacc
    30181 ctacgctcaa cctttattgt cggcttccct ggcgagacgg aagaagattt ccagatgcta
    30241 ctcgacttcc tgaaagaagc acgtctggat cgcgttggct gctttaaata cagcccggtt
    30301 gaaggtgcag acgccaatgc cctgcctgac caggttccgg aagaagtgaa agaagaacgc
    30361 tggaaccgtt tcatgcagtt gcagcagcag atttccgccg agcgcctgca agagaaagtg
    30421 ggccgtgaaa ttctggtgat tatcgacgaa gtggacgaag aaggtgcgat tggtcgcagc
    30481 atggcagatg caccggaaat cgacggcgcg gtttatctca atggtgaaac caacgttaag
    30541 ccgggtgata tcctgcgtgt gaaagtcgag cacgccgatg agtacgattt gtggggtagc
    30601 cgggtttaat taatcttaag gcagatctaa caaggatatt gttcttcgat ctgcctctgc
    30661 tacccttccc gatgtcctac ccttacgcac aaaatcccct gacgcataaa tcgagaaaaa
    30721 tgaatcgtac tcgaggcaac tctcactttt tcacaccaaa ttaaagtttt gatcgcaatt
    30781 acttgaaata agcgtaatgg ttactatagt aaacattacc acaaggatgg atgtatgggt
    30841 gtgtataagg caagacgatt cagccaaagc acgaaaaaac ttggtattca cgataaagtt
    30901 ctcatggctg ccgctgaaga agtgattcag ggcatatggg aggctgattt aggtagcggt
    30961 gtcatcaaaa aacgtttacc cctccaacaa ggaaagagtg gtggagccag aacaattatc
    31021 ttttttaaat cagcaaatca tgtgtttttt tatgatggtt ggtccaagag tggtctaagc
    31081 agtaaaggaa ccaaagaaat cgaagatgat gaactggcgg cctacaaaaa aatggcaaat
    31141 gcatttctgg cctttagcaa taagcaaata gaggatttga tcgaaactgg ctttttgatt
    31201 gaggtgaaaa atgagcgata aattacaaat gatgctggaa gatgcaaagg aactgaatgc
    31261 actgggcctg atgtctgata gtgaagttga tgccattgcc gtcatggtca aagctcgtga
    31321 agtcagcgcc cgtatagcca aagtcaaatc aatgacgggt gaagagatca aagccatccg
    31381 cacccgatac ggcatgtcac aatccgtgct ggcacataca atgggaatgt caaaagaaag
    31441 tgtctcgaaa tgggaacgta atgaaataaa accgagcggc ccggcattac gtattttgaa
    31501 tacattggca ataaaagggc cggaagtttt tgcaaagtaa aatgtttgca aatgatttac
    31561 cacattcatt agtacccctt gattaagggg tactaacact attctaattc tactgccagc
    31621 taatctgcgc cctgccgttt ttcttactct tatacagtcg ctcatccgcc agcattttca
    31681 agcgttcgac ggtagtacat tcatttgaat gcgctacacc gatactggtg cttaaattaa
    31741 tgcttcccac ctggaaatga acaatttctt ttagaatcga tagcaactct tccagcgttc
    31801 cacccgtata aatcaccgca aattcgtcac cgccgaagcg gtagacttta cctttatcta
    31861 cggggagctg tgatttaagg attttcacca ctttcatcaa cacttcatcg cccagcaaat
    31921 gaccgaaggt gtcgttaatg tttttgaatt tatcgacgtc aaacaggcac aaataaccgc
    31981 ctttttccgg cagcgctttt aaatcttcgt caaaggattt tctgcccatc gctttggtca
    32041 gatcgtccag gaatgccgag ttatacatac tgtagagttt tttgacatcg gcagccgaaa
    32101 agaaaagccc gccatccagt acgcgttcaa aatggttatt aataatggct tctttctcca
    32161 caataccacg attaatcact ctgcgtacca gatagaggca actggcaccc gatatcactg
    32221 ccaccaatgc ggcaaagaaa ataatcaatg tacatacctg aaaataaacg tcttttttat
    32281 aagcaaatac gtacaacccg gtcggcaaat aggcgaagtg ctccatcgct tccgaaacag
    32341 tgaagatggc gtttttagcg ttatattcat caccggtcac cgatttaccg ttcttgaaca
    32401 gggagaacat caggttctcg ctatccatcg ccagcgattt tgacagtaaa gaaaaacgtt
    32461 ccagaccaag caaaaatatc agactacgat tgtgagttac cggatagagg taatagacga
    32521 aggttttacc atcaaaagag ataaaccctg tatttttgac atgtcgatac ttcacaacct
    32581 gcataatatc tgcacaataa tttttctcga actcctttac ctgaatacat tcgtcactgg
    32641 aaatcagata aggaccgaca tcgtacaaat aagagggtga aaccgagtcg gcgtaagctg
    32701 tgcgtaagcc aaccaacagc tgttcctccg tgggatttag cgtgttgata agatcaattg
    32761 ccacgctacg ccgatattca gcaatatttg ccaccgcaaa agcagtatca ataacggact
    32821 gtttcaggct atacacccgt tcctttacca tttgagtgta gatcccgcag gcaactgctg
    32881 aaatcatgat aaaaatcagc agactgactg taagtacgaa cttattgatt ctggacatac
    32941 gtaaactact cttttactaa ttttccactt ttatcccagg cggagaatgg catgggtttc
    33001 tggtacagat acccctgagc ctgaacacat cctaatttga ttaattcgcg cgcaacgtct
    33061 tcgctctcta cgccttccgc aatcaccggg atccggttat agcgtgccag atcgaaaata
    33121 atgcgcacaa tttttcccgc agtaccaaaa atatcgccgg taaactgcga gtccaccttt
    33181 atgctgtctg gattaatttc ataaaaacgt ttcaggtttg acaaaccggt gccaaagtca
    33241 tcaatcgata tccgtacccc ttgtttacgc agttgttcaa tattgagata aaaatgttca
    33301 ttaattcgct caatcacggt ttcagtgatc tcaacacaaa ggctgttcgc cttcaggcca
    33361 ttttctttca tgatggtagt aaaacgctca tgaaagtgtg gttcgtttaa ctgccagggc
    33421 gaaacattaa tatgcagtaa gaaatcatcc ttcagcgacg cacgctgact aaaatgtctg
    33481 aattcaccca tcgcggtttt aataatttga tagcccagct cattgataaa cccgatatct
    33541 tcagcaatgg taatgaactt cagcggtgaa atgatccccc gctccgcaga cacccaacgg
    33601 cacaacgcct ccgcgcccag aatggcgcgg gtgttaatat caacgatcgg ttgatagacg
    33661 aggaatagtt cgccaatttc cattgcatgg cgaatattac gcgcaatcca gatattctct
    33721 tctatttcgt tgagtttcgc atcatcacag aaaataaact ctgagaacat atggttgcgg
    33781 atactggaaa tcgccagttt cagcttttct gcgtaactcc aggcgttttc cccctgcaaa
    33841 ggttcacaaa tagcagactt atggatgcgc agattgcggt agccaaaact gctgtcaaaa
    33901 tcgtttaccg tcgctatctt acgggtaaac gtctgtacat tttcccggca caggagtaaa
    33961 tagagatcgg cattaaatgt cacgatatcg cagcattgcg catatttctc ggcaattcgc
    34021 tgacacaatt tcctgacgat tttattgcgt tcatcaacac caaacaccgc ttccacagct
    34081 tccagaccac acagttagat gatccccaca gccagatact gattattgtt ggattcaata
    34141 atcgccatat ttttaataaa accgctttcc tgatcgtaag agatcagtga gagcaattta
    34201 tttgaccgtt caatttcggc atctttcatt tccgcgatcg aatttttgat gctgataatc
    34261 tctttcgcat cgccttcgcg aattttcaga gcctcgatct gatccgttga gtcgggcaaa
    34321 aacgccagcg tattaaccag ctcggtcatt tcagaaaatt gccgacggaa cacacgagtg
    34381 gtcattaaat aaaccaccaa caatacgata ataatgaacg caaacggcag ataaaaaagt
    34441 ttatctgatg cgttcaataa attaccacca ggataataa
//
LOCUS       DAZUCC010000025        34340 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZUCC010000025 DAZUCC010000000
VERSION     DAZUCC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.025"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACHY09_001087"
     CDS             complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACHY09_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFS3884089.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFSVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2096..2962
                     /gene="yghU"
                     /locus_tag="ACHY09_001088"
     CDS             2096..2962
                     /gene="yghU"
                     /locus_tag="ACHY09_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFS3884090.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHY09_001089"
     CDS             complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHY09_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFS3884091.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHY09_001090"
     CDS             complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHY09_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFS3884092.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHY09_001091"
     CDS             complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHY09_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFS3884093.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMLLSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4161..4655)
                     /gene="hybD"
                     /locus_tag="ACHY09_001092"
     CDS             complement(4161..4655)
                     /gene="hybD"
                     /locus_tag="ACHY09_001092"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFS3884094.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHY09_001093"
     CDS             complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHY09_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFS3884095.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHY09_001094"
     CDS             complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHY09_001094"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFS3884096.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSAMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICTYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHY09_001095"
     CDS             complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHY09_001095"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFS3884097.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGSGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHY09_001096"
     CDS             complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHY09_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFS3884098.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHY09_001097"
     CDS             complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHY09_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFS3884099.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHY09_001098"
     CDS             complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHY09_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFS3884100.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQSYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11269..12309
                     /gene="gpr"
                     /locus_tag="ACHY09_001099"
     CDS             11269..12309
                     /gene="gpr"
                     /locus_tag="ACHY09_001099"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFS3884101.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNAIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12349..12843)
                     /gene="yqhA"
                     /locus_tag="ACHY09_001100"
     CDS             complement(12349..12843)
                     /gene="yqhA"
                     /locus_tag="ACHY09_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFS3884102.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13034..13918
                     /gene="yghA"
                     /locus_tag="ACHY09_001101"
     CDS             13034..13918
                     /gene="yghA"
                     /locus_tag="ACHY09_001101"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFS3884103.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAITDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     ASYVTAEVHGVCGGEHLG"
     gene            complement(13957..14382)
                     /gene="exbD"
                     /locus_tag="ACHY09_001102"
     CDS             complement(13957..14382)
                     /gene="exbD"
                     /locus_tag="ACHY09_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFS3884104.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTV
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14389..15123)
                     /gene="exbB"
                     /locus_tag="ACHY09_001103"
     CDS             complement(14389..15123)
                     /gene="exbB"
                     /locus_tag="ACHY09_001103"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFS3884105.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15375..16562
                     /gene="metC"
                     /locus_tag="ACHY09_001104"
     CDS             15375..16562
                     /gene="metC"
                     /locus_tag="ACHY09_001104"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFS3884106.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLLPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16702..17361
                     /gene="yghB"
                     /locus_tag="ACHY09_001105"
     CDS             16702..17361
                     /gene="yghB"
                     /locus_tag="ACHY09_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFS3884107.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17401..18300)
                     /gene="yqhC"
                     /locus_tag="ACHY09_001106"
     CDS             complement(17401..18300)
                     /gene="yqhC"
                     /locus_tag="ACHY09_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFS3884108.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18494..19657
                     /gene="yqhD"
                     /locus_tag="ACHY09_001107"
     CDS             18494..19657
                     /gene="yqhD"
                     /locus_tag="ACHY09_001107"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS3884109.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19762..20589
                     /gene="dkgA"
                     /locus_tag="ACHY09_001108"
     CDS             19762..20589
                     /gene="dkgA"
                     /locus_tag="ACHY09_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFS3884110.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20789..21715
                     /gene="yqhG"
                     /locus_tag="ACHY09_001109"
     CDS             20789..21715
                     /gene="yqhG"
                     /locus_tag="ACHY09_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFS3884111.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTTNNPDEDM
                     RIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21766..22023
                     /gene="yqhH"
                     /locus_tag="ACHY09_001110"
     CDS             21766..22023
                     /gene="yqhH"
                     /locus_tag="ACHY09_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFS3884112.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSDANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22065..24284)
                     /gene="ygiQ"
                     /locus_tag="ACHY09_001111"
     CDS             complement(22065..24284)
                     /gene="ygiQ"
                     /locus_tag="ACHY09_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFS3884113.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            complement(24395..25807)
                     /gene="ftsP"
                     /locus_tag="ACHY09_001112"
     CDS             complement(24395..25807)
                     /gene="ftsP"
                     /locus_tag="ACHY09_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFS3884114.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPTLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25882..26619)
                     /gene="plsC"
                     /locus_tag="ACHY09_001113"
                     /gene_synonym="parF"
     CDS             complement(25882..26619)
                     /gene="plsC"
                     /locus_tag="ACHY09_001113"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFS3884115.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26853..29111)
                     /gene="parC"
                     /locus_tag="ACHY09_001114"
     CDS             complement(26853..29111)
                     /gene="parC"
                     /locus_tag="ACHY09_001114"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS3884116.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29249..30856)
                     /gene="ygiS"
                     /locus_tag="ACHY09_001115"
     CDS             complement(29249..30856)
                     /gene="ygiS"
                     /locus_tag="ACHY09_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS3884117.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGIKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(30965..31447)
                     /gene="ygiV"
                     /locus_tag="ACHY09_001116"
     CDS             complement(30965..31447)
                     /gene="ygiV"
                     /locus_tag="ACHY09_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS3884118.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(31500..31892)
                     /gene="ygiW"
                     /locus_tag="ACHY09_001117"
     CDS             complement(31500..31892)
                     /gene="ygiW"
                     /locus_tag="ACHY09_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFS3884119.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32044..32703
                     /gene="qseB"
                     /locus_tag="ACHY09_001118"
     CDS             32044..32703
                     /gene="qseB"
                     /locus_tag="ACHY09_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFS3884120.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGDPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32700..34049
                     /gene="qseC"
                     /locus_tag="ACHY09_001119"
     CDS             32700..34049
                     /gene="qseC"
                     /locus_tag="ACHY09_001119"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFS3884121.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(34095..>34340)
                     /locus_tag="ACHY09_001120"
     CDS             complement(34095..>34340)
                     /locus_tag="ACHY09_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFS3884122.1"
                     /translation="NKYDWMLESGDDICSIPSVSGEDRILQAMIAAFFLLTPLIIIIL
                     RKLFVREMFEFWLYVISLVICLVCGWWLFWGRFIFCY"
BASE COUNT         8401 a         9202 c         8685 g         8052 t
ORIGIN      
        1 cataatcagt acggggttgc tacaacatgt attactcttt caccacaatt agcggttcaa
       61 tatcactctc ttttttgatg accagtgatt catcaccgcg caaacacgtc ccaccgtagt
      121 tgccgccaac ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc
      181 acagttgctg atagatgttt ttctgctcgg caaatttacc gctggttttg tccagcacct
      241 cttcatgatg gctgacgagg tcgatgttac tgccacagcg accggcgatc ggtttcactg
      301 cataaccggt tttcaccagt tcatcattca cggagaaatc ggtatccagc aggtaacgat
      361 ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcactgtcc
      421 acagaggttc aaagaccagc acttccgggc gcagcaatac gtcgataaga cgcacttcat
      481 tttgcggatg accggtacgg atcggcaccg cagcaaactc acggtcgcta acttcacgaa
      541 tctgatcaaa cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt
      601 ccccatcaat cagttgcccg gcagcatccc agcccagttc atccagcccg cgcaagatac
      661 gcgtttcaaa gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct
      721 cgatatcttt gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac
      781 cagccagttc gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct
      841 ccgcccaacg ttcgaggatc aagccagctt cggtatgaca ggaggcggag tcggcgttgt
      901 attcgtagac cttcaggcca cgttcgtcca tacagaaatc catacgaccg gtgatcatat
      961 ggtgacggcg acgctgccag gagagacgca aacgtggcca gaggattttt gggatgtcga
     1021 acagcgccag caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcaggt
     1081 gcagttcgtt ggtggcttta atcagctcct gctctgcgct ctcggtaatg gtgtagtaat
     1141 gatacggatc ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgtagcggat
     1201 ctttttcatc cagccattta ccgtcaaact ggcctttgtt ttccaggcgc gctccgctga
     1261 ttttcaacag ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct
     1321 ggatcatcca gccgagaatg gtggtgtcat caaaagtgtc tttaagggta tagcagccgt
     1381 tttcgaccac catctccagc tcgcgcgtcc actgttgccc ttgcggtaac ggggaatgaa
     1441 tcacgttctg ttccgcaata cggactttgt tgccatgcaa ttgggtaatg atggcgacat
     1501 gaccagtgtc tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg
     1561 cacgcggcga gccgttagga aatgcctgca atggcaggat gttgtcatta accacttcac
     1621 gcaggaagcg cagcgagaaa atctcccacg ccatacccac gtcagtgaag accacaccgt
     1681 aattcagaaa gagaaaacgg cgagcaaatt caacgcattg ccatttgtgg cccatatatt
     1741 cgtcgtcgat atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac
     1801 tgtaatctga agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg
     1861 gggcatcctg gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc
     1921 agttggcgta attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca
     1981 gactaatcat acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt
     2041 acagcaagtg acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac
     2101 agacaacact tatcagcccg cgaaagtctg gacgtgggac aaatccgccg gcggcgcgtt
     2161 cgccaatatc aatcgcccgg tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa
     2221 acacccattg caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct
     2281 tgaggagctg ctggcgctgg gcgttactgg tgcagagtac gacgcctggc tgattcgtat
     2341 tggcgatggc gatcaattct ccagcggctt tgtcgaagtg aacccaaact cgaagatccc
     2401 ggcgctgcgc gatcatacgc ataatccgcc gatccgcgtg tttgagtctg gatcgattct
     2461 gctttatctg gcggagaaat ttggctactt cctgccgcag gatctggcaa agcgtactga
     2521 aacgatgaac tggctgttct ggctacaggg ggctgcaccg ttcctcggcg gtggttttgg
     2581 tcacttttac cattacgcgc cggtgaaaat tgagtacgcc atcaatcgct ttaccatgga
     2641 agccaagcgc ctgctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcagg
     2701 cgatgagtac accattgcgg atatggcgat ttggccgtgg tttggcaacg tggtgttagg
     2761 cggcgtgtat gatgccgcag agtttctgga tgcgggaagt tataaacacg tacaacgctg
     2821 ggcgaaagaa gtgggcgaac gtccggcagt gaagcgtggg cgtattgtta accgcatcaa
     2881 cggaccgctg aacgagcagt tgcatgaacg ccatgacgcc agtgatttcg agacgaatac
     2941 ggaagataag cgtcaggggt aagggttggt attcgtcgca gcaagccatc caggccggat
     3001 aaggcgttca cgccgcatcc ggcaatcgag cacaatgcct gatgcgacgc tgccgcgtct
     3061 tatcaggcct acaaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag
     3121 cgcgtctaat gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg
     3181 taccagtacc cactggccca gcagatcggc agggttacct tcacaaatca gggcgatatt
     3241 cacgtcgcgc ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac
     3301 agccagcacc tggcctggaa cgccaataca cattgttaac tccggttatt caacttcaat
     3361 acttttgacg attagcgaat cgccggtatc gacacgcaac cgctcgccat gacagatcgg
     3421 acactgcgca tcgtgctgat gaatctccac cacctggctg caatcccagc accaggcctg
     3481 ggcgggttta tagacgatat gtaaatcgca cccttgcgcc accgttccgt ggcagacaat
     3541 ttcaaaacta aaacggacgg cgctctcctc aacgcaggag agcgcaccaa tttccagcca
     3601 cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc cgttggataa tttcaacggc
     3661 gctctggcaa agcgacaact catgcatttt cgccactcct gcggccaaat agcagggcac
     3721 gacgccctgc gtgtggtaca tccggattgg tgactggcag cgaaaggagc attcgggcgc
     3781 agtcatccgt cagccgttgg ccctcttcaa tcgacatgct gtgcgaaagc ggcgacatca
     3841 gcgagcagga gaggtattgc gaaacaccat ccagttcacc aacagtaaag gtcatagtgc
     3901 catacggcag ttgcagacca attttttcac tcactttgcg cagcggccag agttgatccg
     3961 ggccggggaa gataactgca ctcagcatcc acggggtgat cacacacccc gtccactgac
     4021 cttcgaacag cgtaaagtca gaaacataca ccggcattga aggatgcaga aaagagagat
     4081 catgcatcga acgccgggca atttcttcaa aagctgcctg tacttgcgcc ttcggggagg
     4141 tctggaaacc tgctatctct tcagtcatga atcgcctccc gtgggatggc ttccacgcct
     4201 gattcacgca gcgcagccag aacctgctca agcgcaggtt caatcatcgc ttcgaccgtc
     4261 ggcgtcaagc caatgtgcgg ctccagtgat tccgggatca cgccgaccag ggtcagcttt
     4321 ttcggaaact caccggtgaa gcgcagggcc gacaatacgt cggccaggcc aagctgatgc
     4381 ggagagattt tgttggtaaa caacgccgga acttcttcat ctcgcaggat catcatcgtt
     4441 cccggcgcgt tctttttcga gacaatggca tccgcaataa tcagatgatc gcgatttgcc
     4501 atgtcgccaa gcagctccat tcccgccgtt ccgccatcga ggatctcaac ataatccggc
     4561 agaatgtatc gttgctctaa cgcttcaaca attcgcacac cgatggcttc atcggtcagc
     4621 aaaatattgc caacccctaa gactaaaata cgcattacag aaccttcact gaaaccactt
     4681 cgttgccgtc ggcatccact acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa
     4741 tggtacgcac cacttccagc ggtttattcg gatcggcaac cggtgtaccc accagcgact
     4801 gctcgtaagg accgacgtca tcattgaagt tacgcgggcc agagttccag gttgatggaa
     4861 caactgcctg gtagttactg ataataccgt ctttgatcac catccagtga gagagcatac
     4921 cgcgcggtgc ttcaaggaaa ccaacgcctt tgaactcacc tgtagccgga atgttcggct
     4981 tcacaaaggt ggtgtgatcg cctttgccga tattggtgat cagtgcactg tattgatttt
     5041 gcaggatatc ctgcaattcg cagcagtgaa cggtacgacc aataatacgg cccagcgtgg
     5101 agtgcagctg cgccacttcc agcgtgttgc cagtcagttt ctgataaatc gcaacgattt
     5161 cattcagttt gttctgggta gactcgcgtc ctgccgccag tttcaccagc atgttagcca
     5221 gcggacccac ttctaccgtt ttgccgtaga aagtcggtga tttcacccag gaatatttac
     5281 cgtcgtcaga ccaaccatca taagccggaa tggtggtacc ttcccacggt gcctgcggcg
     5341 cttcgtcttt ataccaggag tgcttcgcgc tctcctgaat gcctttaatc aggtattcat
     5401 cggagtggga agtgatcggg cgatacgagg acagatccgc attctcaatg tagccgcccg
     5461 ggaacaggaa gctgccgttt ttgctgtcgg tcgggaattc cggcacgctc aggtagttca
     5521 ccgcaccttt accgcgcgtc agccattccg ggtagaacgc ggcgataact gcggtatcaa
     5581 ccttgtaaac ctgctcaaca aagtcgctca gtttatcgat gaaagacttg atgtacatca
     5641 ggcgctcaag gttcagcacg cccagaccgt cgaggttgat cgggttagcg accccaccta
     5701 ctgccaggtt ctgaatgtgc ggcgttttac cgcccagcag cgccacgacg cggttagcgt
     5761 cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc aatcaggttc acttccggcg
     5821 gcagtttcat cgccgggtga ccccagtagc cattggcgaa aatccccaac tgaccgctgg
     5881 caaccagatc tttaatcttg ttctgaactt tggtgaactc ttccggactg ttcagatgcc
     5941 aggtcgaaac gcctttcagc atttcagagg ctttggttgg gtcagcttgc agtgcagaag
     6001 tgatgtccac ccagtccagc gccgagagct gatagaaatg aacgatatgg tcatgcgtgg
     6061 tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt aaccggaacg tcgatattca
     6121 gtgcactttc tgccgcacga acggaagaga gcgcgtgagt agtcgtacat acgccacaga
     6181 tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc
     6241 cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac gccattttcg atttcgcaat
     6301 cgatgcgtaa atgcccctca atacgggtta ccggatcaat agtaattctc tggctcatgc
     6361 tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag
     6421 acgaatgagt acgatgtaag tgcaaatctc aatagccaca aaaccaatag aaatcaacag
     6481 ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg ttgaatgcca ccagcgaata
     6541 ggtcagacgc caggttgcac aacctaacag cgcgctcagt gctgacagga acagcatgcg
     6601 ggaatcatta cgcagcttcg ccacacgcag aacgaccagc gggaagagca tcagcaggac
     6661 ttcaatccag aacatcgcgg agtagaagtc accggcaaac gctaacgaca gcttgtcgcg
     6721 atagataagc tcgccaaagc gcagcacgat gaaaatcgcc agcaacacac tgatggtgtt
     6781 ggtcagcttg acgaacaggc tcttttcatc tggaccattg ccacgcagac ccgcctgcac
     6841 caacgaacct tcaaagatga caatcgagaa gcccatgatg aacgccgtca gcagcgagaa
     6901 cagcggcaac atttcatagc tctgccacaa cggatgcact ttgtagcccg ccgagatcat
     6961 cagcgacccc attgaagact ggtgcatagt tggcagcagc gcaccgagcg cgataatgaa
     7021 gaacatcacc ttgttcagac gctgtagcga caccttccag cccaatcgtt caaacaatgc
     7081 cggagcaaac tccagtgcca tcacgccgat gtagatggtc atacagaccg ccgtctcgaa
     7141 cagtaccgag ttcacgttga agtgacccgg aatgtagaag tacggcaggt tccagtagcg
     7201 accaacgtcg atggtgatcg acaagccacc cagtgagtaa ccgaacagac tcgccaacag
     7261 cgccggacgc accagcggat ggtattgccc acggttaaag acgtataccg cccacgccag
     7321 cgcccagccg ccacaggcaa agccggtgcc gatcaacagg tcaaacgcga tccacacgcc
     7381 ccacgggaag ccgccgttca ggtcagagac agagcccaga ccgaacacca gacgcttcac
     7441 aatcaggagc atacagatga cgattaacgg tccaaaaatc atgaccggtt tactgatgat
     7501 tttgccgccc agcggttgtg gatcatgact catgatcgtc tcctccgtcg tgatggtcgt
     7561 ttttggtgtt gcgacgaacc agcacggtta agcccgccag cacagccagt ggtagcatca
     7621 tgcctttata cagggtgtgt tgaatatttt cggaacgcgc accggtagaa agatcgtcca
     7681 gtttcggcag gtcgagattt tcgtaaggca caccggtcag taccagaacc tgagtaccgc
     7741 cgccctcttt ctcgccgtac agatgcggat aatatttcgg caccgtatgc aggtaagtgt
     7801 cgccagattt cagcgtctga cgcggatagt ggtattcgct gcctggcttc agcgccagac
     7861 gttttttcgc ctcagccatc agctcttcac gcgtaccaaa aatcaccgcg cccgccgggc
     7921 acacttctac gcagccaggc agaccgcctt tatcaagacg ttccacacct ttctggttgc
     7981 acagttcaca cttatgcagc gcaccaaatg ggttgttgta gtcgtacttc ggcacgttgt
     8041 atgggcaggc gaccatgcag taacggcagc cagtgcacac atctttgtcg taatggacaa
     8101 tgccggtttt cggatctttt ttcagcgcag agaccgggca cacagagaca caattcggat
     8161 cgacgcagtg catacactgt ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt
     8221 tgacccccga gccgctggtc cacacctgaa tgatgttatt ggtgtacggc gacagtttgt
     8281 cgttgttcga ccaggtctgt tccccttgcg ggttacgttc agggaaattg atatcctgac
     8341 acttggtgac gcaagcctgg cagccaacgc acaaggtcga gtcgtacaac atccccagcg
     8401 atcccggaat tggcgggcgg ttttcagcag ccgcatgact gacagacggc agcgcgcccg
     8461 tcagcaatgc cccgcaggag gctgctttaa taaaattacg tctgttcacg gttattctcc
     8521 ccgtgagtca gcgttatctt tcttttgctg acgacccagt tcacgcaccg ccatcacgct
     8581 gacaccggca accagcccaa ccacaccgcc gagcaaacca atagcgcccg cagagacgtt
     8641 gccgccttct ttagcgttaa cgtccggttt ctgtgaacgc ggagtctgat tttcgacgtt
     8701 ggcaagctga tggatgcctt tatggaagcc gataccttct tcgttacagc catagcacgg
     8761 gtgaccaatc gctaccggcc acacgccgcc aacgtcgcag aattgcagcg ttgagcagtt
     8821 gccgtatgtt tctggccctt tacagccgag gtggtacagg caccagcctt cgcggtggcc
     8881 ttcatcaccg aactctttgg caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg
     8941 ttcgtgaatc agacggccat aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg
     9001 cttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca
     9061 gcccggaata ttgataacgg ttttgcctgg cagaacttct tgcaggctga ctgcgccagt
     9121 tgggttaact ccagcggcgg caacaccgcc ccacgcagag caggaaccga tagcgataat
     9181 ggctgctgcg ccttccgccg ctttgcggat gtgatccaca atcggctcac cggcaaccat
     9241 gcaataaata ccgttatctt ttaatgggat ggaaccatcc accactaaca catactgccc
     9301 tttgtacttc tcgagagcgt tatgtttgtt ctcttcgacc tggtgaccga aagcagcaga
     9361 aagcacctcg tgatactcca gagagatagt ctccagcacg aggttttcta ccgttggatg
     9421 cgttgcacga agcagagatt ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac
     9481 tggcggacgc tgcgggttag taaccgattc ggccatctct gcagcggctt tgctacttaa
     9541 ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg
     9601 agaatggatg agggtgttat ctccagtcat ttatagttat tccgttgcga agacctggca
     9661 tatattttgc ctcaatcgca aaatcaataa tgcgatcgat gcgccattta ccacacattt
     9721 attatggtta tcgatatcat gatactgcgg cggggcgcta aaacgaaggg aagaagcatt
     9781 agtgtaatta attaaagcaa gataatacgt atgtttgatc aattttcgag gcgataaccg
     9841 gccataaaaa agcgcaacgt agaacaggaa ttattctcac gaaagaaatc cattaccatc
     9901 tctttatcca gtccatagcg gcgcgtcaga atacccgctt cccaggcgct aagctggcga
     9961 tctccggtct tttcgtacat ccggtgtgag tagcctaata caaaaccgcg tttataatcg
    10021 gcacagtatt ttgtcacatt aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt
    10081 aagcctttac caaaatggtt attcatcgtt cacctcattc gctatatatt gccgtatata
    10141 gcgatatttt aggcaaagaa cgacatttgc gcacgctatt ttttcatgct tgtttaagac
    10201 gtaatcctgt aagagataaa cccatcagga gagatattta tcgaaccatt tcagcgtcct
    10261 ttgccaggca agatcggcgg cagatttgtc ataacggggc gtggaatcat tatggaatcc
    10321 gtgattaacc cccggatata tatacgcctc atagacctta ttattggctt tcaacgccgc
    10381 ctcgtaagca ggccagccct cgttgattcg ggtgtccagt tccgcgtagt gcaacagtaa
    10441 aggcgcttca atcttcgcca catcggcagt gggtgcctga cgaccataaa acggcaccgc
    10501 gcaggccagt tccgggtaag cgaccgctgc cgcgttcgat acgccaccgc catagcaaaa
    10561 tccagtaata cccactttgc ctgtcgcttg cggatagctt tgcataaact caatcgcggc
    10621 aaagaaatca ttcatcagtt tggttggatc aacctgctgt tgcagctcac gacctttatc
    10681 atcattaccc ggataacctc caacggaact taagccgtca ggtgccaggg cgatataccc
    10741 cgccttcgcc actcgccgcg tcacatcttc gatatacgga ttcagccctc gattctcatg
    10801 caccactacc acggctggcg ttttgccgct catttttgcg ggcttcacca gataaccccg
    10861 tacctcgccg tgaccatttg gcgagggata cgtgatgtac tctgcaacaa tttccgggtc
    10921 ggtaaactct acctgagtcg ccagcgcata atttggcttg agcaaatcaa acagcgctaa
    10981 tgccgtcatc ccgcccaccg catacttcgc cgcaaggttg aggaactcac gtttcgagat
    11041 tttcccgtga gcgtaatagt cgtagtaatc cagcaactct tgtgggaaat ctttggcggt
    11101 taaacgcggc atcgttgcac tcctcaactg gtgttttttt aagcaaagca taagcgcgtg
    11161 tttttgccca gtttttcgtc actctgtgag ccagactacg ggatacgcgc tggcgaatcg
    11221 ctaaactaga aacattgttt cgaaattgaa cgatggaaag gagaggtcat ggtctggtta
    11281 gcgaatcccg aacgttacgg gcagatgcag taccgctatt gcggaaaaag tggtttacgc
    11341 ctgcccgcgt tatcgctcgg tttatggcac aatttcggtc acgttaacgc gctggaatca
    11401 cagcgtgcga tcctgcgtaa agcgtttgat ttaggcatta cgcactttga tttagccaac
    11461 aattacgggc cgcctccagg aagcgcagaa gagaactttg gtcgcctgct gcgcgaggat
    11521 tttgccgctt atcgcgatga actgattatc tctaccaaag ccggatacga tatgtggccc
    11581 ggcccgtacg gctctggcgg ctcacgtaaa tacctgctcg ccagcctcga ccaaagcctg
    11641 aagcgtatgg ggcttgagta tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg
    11701 ccgatggaag aaaccgcctc tgcgctggct catgcggtac aaagcggtaa ggcgctgtat
    11761 gtcgggatct cctcttactc gccagagcgg acgcaaaaaa tggttgagtt gctgcacgag
    11821 tggaaaattc cgctgttaat tcatcaacct tcgtacaatt tactgaaccg ctgggtggat
    11881 aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg gctgcattgc ctttactcca
    11941 ctggctcagg gattgctgac cggaaaatat ctcaacgcta ttccgcaaga ttcacggatg
    12001 catcgtgaag ggaataaagt tcgtggtctg acgccgaaaa tgctcaccga agccaacctc
    12061 aacagcctgc gcttattgca tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg
    12121 gcgttaagct ggttgctgaa agatgagcga gtgacgtcgg tattgattgg agccagccgc
    12181 gcggagcaac ttgaggagaa cgtgcaggcg ctgaataatc tgacatttag caccgaggag
    12241 ctggcgcaga ttgatcagca tatcgccgat ggagagctga atctgtggca ggcgtcttcc
    12301 gataaatgac ctgttaataa cgggcgcgag aaccgcgccc gaatcagatc agtgattatg
    12361 acgagtcagt cggtcaagat agcccatcac aaatgcagag agcacaaacg tcagatggat
    12421 aatgacgtac cacatcagtt tgttatcagg gacatttttc gcatccataa agacgcgcag
    12481 taagtgaatg gaagaaattg ccacaatcga cgctgctact ttgtttttca gcgacgttgc
    12541 gtccattttc cccagccagt tcagcttctc tttgttctca gagatatcga gttgtgagac
    12601 gaaattctcg taaccggaaa acatcaccat caccagcaaa ccgccaacca gcgtcatatc
    12661 caccagcgac agcaacacga ggatcaaatc tgattccgcc atcgagaaga tattcggcag
    12721 tacgtgaata atctcctgga agaacttcag cgccagggca actaacgcca gcgaaaggcc
    12781 aaagtacacg ggggcaagca gccagcgaga agcatacatt gcattttcaa gaaaacgttc
    12841 catacattcc tgtcgtgtag ttaaaacggg caacagtata tcgcaattaa gtaaacagca
    12901 cgcaacagca aatcaacata tacgttaaac ctttgtcgca taattctgag ggttagggat
    12961 cttcctggtt cccttatcaa cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga
    13021 agggagaaaa atgatgtctc atttaaaaga cccgaccacg cagtattaca ctggtgaata
    13081 tcccaaacag aaacaaccga cgccaggcat ccaggcgaag atgacaccgg taccggattg
    13141 cggcgagaaa acctatgttg gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg
    13201 gggcgattcc ggaatcggtc gcgctgccgc cattgcttac gcgcgtgaag gggctgacgt
    13261 ggcgatcagt tatcttcccg tggaagaaga agacgctcag gatgtcaaaa agatcattga
    13321 agaatgcgga cgcaaagccg ttctgctgcc aggcgattta agcgatgaga aattcgcccg
    13381 ttcgctggtt cacgaagcac acaaggcgtt aggcgggctg gatattatgg cgctggtcgc
    13441 cgggaaacag gttgccatta ctgatattgc agacctcacc agcgaacagt ttcaaaagac
    13501 ctttgccatt aacgttttcg cgctgttctg gctaacccag gaagcgatcc ccctgctacc
    13561 gaaaggtgcc agtatcatca ccacttcgtc aatccaggca taccagccaa gcccgcattt
    13621 actggactat gcggctacga aggcggcaat tctgaactac agccgtggcc tggcaaaaca
    13681 ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca ggcccgatct ggacagcact
    13741 gcaaatttcc ggcggacaaa cgcaggataa gatcccgcag tttggtcaga aaacgccgat
    13801 gaagcgtgcg gggcaaccgg cggaactggc ccctgtatat gtttatctgg caagtcagga
    13861 ggcgagctac gtcaccgcag aagtgcatgg cgtgtgcggc ggcgagcatt taggctaaaa
    13921 aaatgcccgg ttgtgaaaag caaccgggca tcatttttat ttcgctttgg cagtttcttc
    13981 gccgaccaga cctatcttca ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa
    14041 cgtctcgtaa tcgacggttt tatccgctcg gaagaagacg gtggtgtctt tcttgccttc
    14101 ggttaacgca ttcaacgccg taatcattgt ttcatcggtg accggatcgt taccgataaa
    14161 catcgagttg tctgccttca ccgacagata aaccggtttt tccgggcgcg gctgcggcgt
    14221 gctggtagaa gcaggcaagt tcaccttcac atctaccgtc gctaacggtg ccgccaccat
    14281 aaagataatc agcagaacca acatcacgtc gataaacggc gtcacgttga tatcatgcat
    14341 ttcgccgtta tcgtcgaggt tttcgttaag atgcattgcc atcggacatt atcctgcgcg
    14401 taatttttgt gcgacacgaa ccggatgcgc agcggcgctg gcttccagat ccaggtcacg
    14461 gctttgcagc aacaatacct gcgctgcaac atcacccagc atcgctttaa agccgccaat
    14521 ctggcgtgca aatacgttat agataacgac cgccggaatc gctgccacga ggccgattgc
    14581 cgttgctaac agagcttctg cgatacccgg cgcaacgact gccaggttag tagtctgcgt
    14641 ttgcgcgata ccaataaagc tgttcatgat gccccagacc gtaccaaaca gaccaacgaa
    14701 cggcgaaatc gcgccgatgg ttgccagata gccgttacca cggcccattt gacgacctac
    14761 tgcggcgacc cgacgctcca gacggaagct ggtacgttct ttaatacctt cgttatcgtc
    14821 gctgccttct gacagttcca gctcattctg cgcttcattg agcaaatgca ggctcaagct
    14881 tttgctacca aaatcagcgg cgatatcgtt ggcctggttt aaggaacgcg cttcagccag
    14941 cagttgctgc tcgcgcttaa gacgacgctt ctgattgaag aactccacgc tcttactgaa
    15001 gaagattgcc caggtgacta cggaggccaa aataagccca atcatcacgc acttaacgac
    15061 aatatcggcg tgctgataca taccccagac ggagaggtcc gtctgcatta aattattacc
    15121 cactgtgtat ctccaggacg caagtcacaa aatctgcgca taataatatc aaaacgacgt
    15181 cgaattgata gtcgttctca ttactatttg catactgccg tacctttgct ttcttttcct
    15241 tgcgtttacg cagtaaaaaa gtcaccagca cgccatttgc gaaaattttc tgctttatgc
    15301 caattcttca ggatgcgccc gcgaatattc atgctagttt agacatccag acgtataaaa
    15361 acaggaatcc cgacatggcg gacaaaaagc ttgatactca actggtgaat gcaggacgca
    15421 gcaaaaaata cacgctcggt gcggtaaata gcgtgattca gcgcgcgtct tcgctggtct
    15481 ttgacagtgt ggaagccaaa aaacacgcga cacgtaatcg cgccaatggc gagttgttct
    15541 atggacggcg tggaacgtta acccatttct ccttacaaca agcgatgtgt gaactggaag
    15601 gtggcgcagg ctgtgtgcta tttccctgcg gggcggcagc ggttgctaat tccattcttg
    15661 cttttgtcga acagggcgat catgtgttga tgaccaacac cgcctatgaa ccgagtcagg
    15721 atttctgtag caaaatcctc agcaaactgg gcgtaacgac atcgtggttt gatccgctga
    15781 ttggtgccga tatcgttaag catctgctgc caaacactaa aatcgtgttt ctggaatcgc
    15841 caggttccat caccatggaa gtccacgatg ttccggcgat tgttgccgcc gtacgcagtg
    15901 tggtcccgga tgccatcatt atgatcgaca acacctgggc agccggtgtg ctgtttaagg
    15961 cgctggattt tggcatcgat gtttctattc aggccgccac caagtatctg gttgggcatt
    16021 cagatgcgat gattggcact gcggtgtgca atgcccgttg ctgggagcag ctgcgagaga
    16081 atgcctatct gatgggccag atggtcgatg ccgataccgc ctatataacc agccgtggcc
    16141 tgcgcacatt aggtgtgcgt ttgcgccaac atcatgaaag cagtctgaaa gtggctgaat
    16201 ggctggcaga acatccccaa gtagcgcgag ttaaccaccc tgccctgcct ggcagtaaag
    16261 gccacgaatt ctggaaacga gactttacag gcagcagcgg gctattttcc tttgtgctta
    16321 agaaaaaact caatgatgaa gagctggcga actatctgga taacttcagt ttattcagca
    16381 tggcctactc gtggggcggg tatgaatcgt tgatcctggc aaatcaacca gaacatatcg
    16441 ccgctattcg cccacaaggc gagatcgatt ttagcggaac cttgattcgc ctgcatattg
    16501 gtctggaaga tgtcgacgat ctgattgccg atctggacgc cggttttgcg cgaattgtat
    16561 aacattgcca cttttggaca attttgcaga cattttattg tgaaaagtct taaattgttg
    16621 cgtccgggat caaggcgtcc cggacgattc aggagtacaa taggcagata aaggcttaaa
    16681 cgctgttcca caggaaagtc catggctgtt attcaagata tcatcgctgc gctctggcaa
    16741 cacgactttg ccgcgctggc ggaccctcat attgttagcg ttgtttactt tgttatgttt
    16801 gccacgctgt ttttagaaaa cggcttgctg cccgcctcat ttttgccagg cgacagcttg
    16861 ttgatactgg caggcgcatt gattgcccag ggggttatgg attttctgcc tacgattgcg
    16921 attctgaccg ccgcagcaag tctgggctgc tggctaagtt atattcaggg gcgctggtta
    16981 gggaatacca aaacggtgaa aggctggctg gcacagcttc ctgctaaata tcaccagcgc
    17041 gccacctgca tgtttgaccg ccacggtctg ctggcgctgc tggctggacg ttttcttgca
    17101 tttgtccgta cgctgctgcc aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc
    17161 cagtttttca actggttaag cggattgctg tgggtcagcg tggtaaccag tttcggctat
    17221 gccttaagta tgattccgtt cgttaaacgc catgaagatc aggtaatgac gttcctgatg
    17281 atcctgccaa ttgccttgtt aaccgctggc ttgttgggca cgctgtttgt ggtgattaaa
    17341 aaaaaatact gtaacgcctg acgattttcc ccattcccgg tagctgtacc gggaacgtat
    17401 ttaattcccc tgcatcgccc gcattcttgc cgcatcttcc cccggcgtca caccgaagta
    17461 acgtttaaac tcacggctaa attgcgatgc gctttcatag ccgacgcgca tcgccgccgc
    17521 gctggccttc atgccgtcgt ggatgatcat catccgcgcc ttatgcagac ggtaattctt
    17581 caaatactgc aacggcgagg tgctggtgac agacttaaaa ttatggtgga acgccgatac
    17641 gctcatgttg gcttctgccg ccagttgctc gacgctcagg ttttcggtgt atttattctc
    17701 aatccgtttc agcacgcgac taatcaggct gaagtgagtc tggcggctga ccagtgccag
    17761 taacgcgccg ccgcaaggtc cggtcagcac gtagtacaga atttcgcgga tgatctgttt
    17821 gccgagaata cgcgcatcca gcggtcgctc catcacatcc agtaaccgct ccgccgcgca
    17881 taaaatctct tctgataacg tggcagagtt aatcccgctg gctgccatcg acggctggaa
    17941 atgctcatct tcgccaatgt ccatcaacag ttcctgtaac tgcaaaatat cgacattgag
    18001 acgcaaccct gccagcggca cctctgacgt ggcataggtt tcgcactcaa acggcaacgg
    18061 caccgtcagc aggagatatt cattagcgtc ataacgaaac acgcgttcat tgatataacc
    18121 gattttatgt ccggaaaaga gaattatgat gccaggctcg tacatcaccg gtgtgcgtgc
    18181 gaaaggcgtc tcgccataca acaaacgcac atcgggcaac agctctgaca aactattttc
    18241 tttatttttc agtttattaa ctttatccgc cagcaagcgg caaatctctt cacgtttcat
    18301 atcgcgtaat ttcttaggaa taatgcggca atttgattgt gcgcaatttt gtagcatttc
    18361 tccagcactc tggagaaata ggcaagacat tggcagaaat gagcattgag aggtacggcg
    18421 ctggcgacca caatgaaaaa catcaggcag accgttctct gccctcatat tgacccagca
    18481 aagggagcaa gtaatgaaca actttaatct gcacacccca acccgcattc tttttggtaa
    18541 aggcgcaatc gctggtttac gcgaacaaat tcctcacgat gctcgcgtat tgattaccta
    18601 cggcggcggc agcgtgaaaa aaaccggcgt tctcgatcaa gttctggatg ccctgaaagg
    18661 catggacgtg ctggaatttg gcggtattga gccaaacccg gcttatgaaa cgctgatgaa
    18721 cgccgtgaaa ctggttcgcg aacaaaaagt gactttcctg ctggcggttg gcggcggttc
    18781 tgtactggac ggcaccaaat ttatcgccgc agcggctaac tatccggaaa atatcgatcc
    18841 gtggcacatt ctgcaaacgg gcggtaaaga gattaaaagc gccatcccga tgggctgtgt
    18901 gctgacgctg ccagcaaccg gttcagaatc caacgccggc gcggtgatct cccgtaaaac
    18961 cacaggcgac aagcaggcgt tccattctgc ccatgttcag ccggtatttg ccgtgctcga
    19021 tccggtttat acctacaccc tgccgccgcg tcaggtggct aacggtgtag tggacgcttt
    19081 tgtacacacc gtggaacagt atgttactaa accggttgat gccaaaattc aggaccgttt
    19141 cgcagaaggc attttgttga cgctgatcga agatggtccg aaagccctga aagagccaga
    19201 aaactacgat gtgcgcgcca acgttatgtg ggcggcgact caggcgctga acggtttgat
    19261 cggcgctggc gtaccgcagg actgggcaac gcatatgctg ggccacgaac tgactgcgat
    19321 gcacggtctg gatcacgcgc aaacactggc tatcgtcctg cctgcactgt ggaatgaaaa
    19381 acgcgatacc aagcgcgcta agctgctgca atatgctgaa cgcgtctgga acatcactga
    19441 aggttcagac gatgagcgta ttgacgccgc gattgccgca acccgcaatt tctttgagca
    19501 attaggcgtg ccgacccacc tctccgacta cggtctggac ggcagctcca tcccggcttt
    19561 gctgaaaaaa ctggaagagc acggcatgac ccaactgggc gaaaatcatg acattacgct
    19621 ggatgtcagc cgccgtatat acgaagccgc ccgctaagct ttttacgcct caaactttcg
    19681 ttttcgggca tttcgtccag acttaagttc acaacacctc accggagcct gctccggtga
    19741 gttcatataa aggaggaacg tatggctaat ccaaccgtta ttaagctaca ggatggcaat
    19801 gtcatgcccc agctgggact gggcgtctgg caagcaagta atgaggaagt aatcaccgcc
    19861 attcaaaaag cgttagaagt gggttatcgc tcgattgata ccgccgcggc ctacaagaac
    19921 gaagaaggtg tcggcaaagc cctgaaaaat gcctcagtca acagagaaga actgttcatc
    19981 accactaagc tgtggaacga cgaccacaag cgcccccgcg aagccctgct cgacagcctg
    20041 aaaaaactcc agcttgatta tatcgacctc tacttaatgc actggcccgt tcccgctatc
    20101 gaccattatg tcgaagcatg gaagggcatg atcgaattgc aaaaagaggg attaatcaaa
    20161 agcattggcg tgtgcaactt ccagatccat cacctgcaac gcctgattga tgaaactggc
    20221 gtgacgcctg ttattaatca gatcgaactt cacccgctga tgcaacaacg ccagctacat
    20281 gcctggaacg cgacacacaa aatccagacc gaatcctgga gtccattagc gcaaggtggg
    20341 aaaggtgttt tcgatcagaa agtcattcgc gatctggcag ataaatacgg caaaaccccg
    20401 gcgcagattg ttatccgctg gcatctggat agcggcctgg tggtgatccc aaaatcggtc
    20461 acaccttcac gcattgtcga aaactttgat gtctgggatt tccgtctcga caaagacgaa
    20521 cttggcgaaa ttgcaaaact cgatcagggc aagcgtctcg gccccgatcc tgaccagttc
    20581 ggcggctaac gtgcaaattc tcccggtggc ggtaatgttc cgctaccgga cttttcagaa
    20641 atcatttatt cccctcgcgt cctgcccatt gttactcttc cttgttcagg aatgccaaat
    20701 ataaggacat catcatgcag agccggaagc tcttaaagga acaactcatc tacacccggg
    20761 ataaacgcaa cggagaggtg aaaaacagat gaaaataata cttctgtttt tagcagccct
    20821 ggcaagtttt accgtacacg cgcagcctcc ttcacagacc gtagaacaaa cagtccggca
    20881 gatttatcag aactataaat cagatgccag tacgccttat tttggtgaaa ccggagagcg
    20941 agcgataact tctgcgcgta ttcaacaggc gcttaccctg aacgacaatc ttacgctgcc
    21001 gggcaatatt ggctggctgg attatgatcc ggtttgtgat tgtcaggatt ttggcgatct
    21061 ggtgttagaa agcgtagcga taacccaacc tgacgccgat catgccgatg ccgttgtgcg
    21121 ctttcgtatc tttaaagacg ataaagaaaa gaccacgcag acactgaaaa tggtggcgga
    21181 aaatggtcgt tgggtcattg acgatattgt cagcaatcat ggcagcgtct tacaagcagt
    21241 taatagcgag aatgaaaaaa cgctggccgc tatagcttcg ttgcaaaaag aacagccgga
    21301 agcctttgtt gccgaactct ttgaacatat tgctgattat agctggccgt ggacgtgggt
    21361 ggtttccgac tcttaccgcc aggcggtcaa tgccttctat aaaaccacct tcaagactac
    21421 caacaatccc gatgaagata tgcgaataga acggcaattt atttacgaca atccgatctg
    21481 ttttggcgaa gagtcgctat tttcacgcgt tgatgaaatt cgagtcctgg agaaaaccgc
    21541 cgattccgcc cgcattcatg ttcgttttac gctgaccaat ggcaacaatg aagagcaaga
    21601 actggtttta cagcggcgcg aaggtaagtg ggaaatcgct gattttatcc gcccgaatag
    21661 cggtagccta cttaagcaga ttgaggcaaa aaccgccgcc agattaaagc aatgagctga
    21721 gttaaataac aattagccgg aacaataaat aaaagggaac actatatgaa aacgattttc
    21781 accgtgggag ctgttgttct ggcaacctgc ttgctcagtg gctgcgtcaa tgagcaaaag
    21841 gtcaatcagc tggcgagcaa tgtgcaaaca ttaaatgcca aaatcgcccg gcttgagcag
    21901 gatatgaaag cattacgccc acaaatctat gctgccaaat ccgacgctaa cagagccaat
    21961 acgcgtcttg atgctcagga ctattttgat tgcctgcgct gcttgcgtat gtacgcagaa
    22021 tgataaaaaa attcccggca gcattctgtt accggggata ttttttaacg tccaaccgcc
    22081 gctttagggc gtttcttcgc accagcattc tgcaggcgag attgcgtaga cgacgctttt
    22141 tttgccgtag caggcgtctg acgctgggtc gccatcggcg tatgtttagt caacgccgga
    22201 cgggtattgc ggttctggcg acgagcctca cgcatctctt caatggttgg cgcaggcact
    22261 aagcaatcgc gacggctgcc aatcagatgc tttttgccca tcgcttccag cgcctggcgg
    22321 attaacggcc agtttgccgg atcgtggtaa cgcaacaacg ctttatgcag gcgacgctgc
    22381 ttgtcgccct tcggtacaaa gacgtcttca cttttataac caatcttcgc cagcgggttt
    22441 ttgccggtgt aatacatagt ggtcgagtta gccagcggcg acgggtagaa gttctgtacc
    22501 tggtcgaggc ggaagcggtg ctttttcagc cacagcgcca gattcaccat atcttcatcg
    22561 cgcgtacccg ggtgcgcgga gatgaaatac gggatcagat actgttcttt acctgcctgt
    22621 ttcgagtaag tatcgaacag ctctttaaag cggtcatagc tgcccatgcc cggcttcatc
    22681 atcttcgata acggcccttc ttcggtatgt tccggggcaa tcttcagata accgccaacg
    22741 tgatgggtcg ccagctcttt gatatagcgc ggatcttcta cggcgatgtc ataacgcaca
    22801 ccagaggcaa tcaggatctt tttaatgcct ttcagatcgc gcgcacggcg atagaggtta
    22861 atcgtcggtt cgtggttagt gtccatatgc ggacaaatat ccggataaac gcacgacaaa
    22921 cgacggcagg tttgctcagc gcgtggcgat ttgcaacgca acatatacat gttggcagta
    22981 ggcccaccga gatcggaaat cacacccgta aaacctggaa cagtgtcgcg gatcgcttcg
    23041 atctcattaa taatcgaatc ttcggaacgg ctctgaataa tgcgcccttc gtgctcggta
    23101 atagaacaga aagagcagcc gccaaagcag ccacgcataa tgttgaccga gaaacgaatc
    23161 atttcgtaag ccggaattcg ggcattaccg taagccggat gcggcacgcg cttgtacggc
    23221 agcgcaaaga cgctatccat ctcttcggta gaaagcggaa tagcaggcgg gttgatccat
    23281 acatagcggt cgccatgttt ttgcatcaat gcgcgggcgc agcctgggtt agtttcgtgg
    23341 tgaagaatgc gcgaagcatg ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa
    23401 ggcagcaaca cgtaggtttt ttcccacggt ttcgggcgcg gcggctgcac ggttacggct
    23461 ttcgcttcct gctttttcgg cgcaacaggt ttgttatccg cgcacggcag atcttcgcca
    23521 tacggatgcg ggatcgggtc gattttccca ggagtatcaa gacgggtgga atccacgccg
    23581 ctccagcctg gcaacgcctc ttttacgata atcgcggtat tacgcacatc gcggatttca
    23641 ctaatcggct cgcccatcgc cagacgatgc gccacttcca ccagcggacg ctcaccgtta
    23701 ccgaacatca gcatgtcggc tttcgaatcc accagcacgg aacggcgcac ggtatcggac
    23761 cagtaatcat aatgcgcggt acggcgcaga cttgcctcaa taccgccgag gatcaccggt
    23821 acatctttcc acgcctcttt acaacgctgg gtataaacca gtgtggcgcg atccggtcgc
    23881 ttacccgcga cgttatccgg cgtgtaggca tcgtcatgac gtaaacggcg atcggcggta
    23941 tagcggttga tcatcgagtc catgttgcca gcagtaacac cgaaaaacag attcggttta
    24001 cccagacgca taaagtcgtc tttgctgctc cagtccggtt gggcaatgat cccgacacga
    24061 aagccctgcg cttccagcat acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca
    24121 tacgcgtcgc cagtaaccaa aatgatgtcg cagctatccc agccaagttg atccatctct
    24181 tcacgagaca tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta ctgcggccag
    24241 gagaacaggt cgcgatccgg ttggatcagg gagatagagc tcattttgct tccagaaatg
    24301 ataaaaaaat aatcaaaggc cggggattat aagccggaac gaaagagaaa tcgaaaggta
    24361 ttccatactc gccctcctcg ggcgagtatg aagattacgg taccggattg accaacagtt
    24421 gccctatcga gccacggtcc gccatttcca gcgtctgact gttgaagtag aacgggaagt
    24481 gcgcccagga aggctgaccg aaataaacca gcagctccac ttgtccgtca acccaaacgg
    24541 tatctttcca gcctctgtct tccgggaacg gcatcgcgcc attcacgtta cggatctgga
    24601 acatcacgcc ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc
    24661 acgttccttg ctgcgcggtg acatcaatac ggttgacgtc ccacagttgt ccattaatac
    24721 ccgggtcatc acccagactg atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag
    24781 ttggcagcag gcgcatcgga agactgtctg tgaccagcgg cagaaggccg gttgggcgca
    24841 gcgttagcac cagggtagaa accagaatgc tggatggctc aaagaagccg cgaatacgat
    24901 caacaatgct cgccgcttca ccacaggtga tcgacacttc atcaccgttg ctcatatcca
    24961 ccagaatctc gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat acaggagcgg
    25021 gcaggaatcc ctgatcgcca gaaatcacat gtaacgggcg accatcgctc atctgtagtt
    25081 gatagcgacg agagttcgac gcgttcaaca gacgcaagcg cacccagcca cgcgagactt
    25141 caacgtacgg gctttgtaca ccgttaacca gcagcgtatc accaacaaag ccgccgcttc
    25201 ccggttcgtt atattccggc gtaccaaagt tatccagccg tttatcctga ataatgaccg
    25261 gaaaatcatc cacaccataa tggttgggga taggcagcga tttgctgact tcatcttcca
    25321 ccagccacat tccggcaagg ccgttataga cctgctgagc cgtgcggttg ggagtattgg
    25381 cgtgatacca cagagtagct gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt
    25441 ttggcgacat catccgtgcc ggaccgccca tcagcgggcc aggtacctgt agcccggcca
    25501 ccgtcattga gacattttct gtcaggcgat tgctgtaaat aagcttaaca tcgtcgccct
    25561 tccagacgcg gatagtcggc cccaggtaac gaccattgat tccccaaacc gatgcgcgtg
    25621 tccctggcgt aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc
    25681 gagattcaag cagcgtcgga acgggtagcg gttgctgttg cccggctgcg ctggccttca
    25741 ggggaacagc gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga cgccgactga
    25801 gtgacatatt tgctccatgt aaaactggct aaagtaatac cgtgcaggaa agtgttcccc
    25861 gcaaaaatac agattgccga cttaaacttt tccggcggct tcgcgctctg cgacttcttt
    25921 atcgagctca gcgatttttt gttccattat cgaacgacaa tgggcagcca actcgcgaac
    25981 ctgatctttg ccatactgac tgacgtcaat tggcggcagc atttcgacaa tcaccagacc
    26041 gttgtgtagc cgattaagat taatcttatt cgaagttgta gagacgcaca cgggaataat
    26101 cgggacgccc gccgcaattg ccgcgtgaaa tgccccggtc ttgaacggca gcaggccgcg
    26161 accacggctg cgggttcctt ccgggaacat ccagatggaa atacggcgtt ttttgaagtg
    26221 attcacgact tccgcaatag tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa
    26281 gttgccggtt aaccagtaca actgcccgaa gaaggggatc cacagcaagc tctttttccc
    26341 taccgtcact gtcggcggct gcacaatgtt cgatgccgtc accatgtcat aattgttctg
    26401 gtggttagca atatagatag catttccgta gctttcagca tccgcaggtt tgcggcactc
    26461 aactttcagg ccgaacagcg gtgcaagacg gccaaacata tgcccaaagg tggccacatg
    26521 tttcgggtta cgcgggctga aaaggcagta aatggagccg aatacacaga ctaagatgct
    26581 gtaaatcacg gtaataataa gacgaaagat atatagcata acaccctctg aaggtctgag
    26641 agactggcat tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc cgctctctgg
    26701 aaaagcgtat tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga atttttatcg
    26761 tttccggggg ttgaatacgc tgccggatga cgacttaacg tttcatccgg cgttccttgc
    26821 aagcgggagg aaacagcgcc ctccccggca tattactctt cgctatcacc gctgctggca
    26881 cggcgaggag agtcgatctc aacacgatcg atacgctgca aaccgcgcat caacgtaccg
    26941 cggcgtccac gttcgccagc gactttctgt agctcttccg gacgcagttt aattttgcgt
    27001 ttcccaacat gaatggtcag cgtgctttgc ggcggcagaa cgtacagttg cgcaagacca
    27061 tcctcgccac gcgcggcttc tgccgatgga atgttgataa tcttgttgcc tttgcccttc
    27121 gacagctgcg gcagatcgct taccgggaac atcaacatac ggcctgcctg agtgattgcc
    27181 agcagcatat cggaagcatc ttcaatcacc accggcggca taacatgggc attttccggt
    27241 aaggtgatca aagccttacc tgcacggtta cgcgccacca gatcgttaaa ggtgcagacg
    27301 aaaccgtaac ccgcatcgga agccatcagc agtttctgat cgtcgctttc catcagcata
    27361 tggtcaacgg tcgctccagg cggcaacgtt aatttgccgg tgagcggctc accctgacca
    27421 cgcgccgacg gcagcgtaat cgggtcgatg gcatagctac gaccggtgga atcaacaaac
    27481 actaccggtt ggttgctctt acctttcacc gccgctttga agctatcgcc cgctttataa
    27541 ttcaggcccg gcgcgtcaat atcatggcct ttagcgctgc gcacccagcc catctgcgac
    27601 agcacaatgg tgacaggttc agacggcagc atgtcgtgct cgctcattgc tttcgcttct
    27661 tcgcgttcct gcaacggcga acgacgttcg tcaccgtagg cttgcgcgtc tgcctgcagt
    27721 tctttcttca gcaggttatt cattttacgc tcggaagcca aaatgccctg caactggtcg
    27781 cgctcttttt ccagctcact ctgctcaccg cgaatcttca tctcttccag tttggcaaga
    27841 tgacgcagtt tcagttcgag gatcgcttcc gcctgggttt cagtaaggcc aaaccgcgac
    27901 atcagcgccg gtttcggttc atcttcatta cgaatgatct caatcacttc gtcgatattg
    27961 agaaacgcca ccagcaaacc ttcgaggata tgcaggcgct tgaggacttt ctccagacga
    28021 tagttcagtc ggcggcgcac ggtatcgcgg cggaacacca gccattcgga gaggatttcc
    28081 agcaggtttt tcaccgccgg acgaccatcc agaccgatca tattgaggtt aatgcgatag
    28141 ctcttttcca gatcggtggt agcaaagagg tggttcatca cctgatccat atccacgcgg
    28201 ttggaacgcg gcacaatcac cagacgggtc gggttctcgt ggtcagattc atcgcgcaga
    28261 tcgtcaacca tcggcagctt tttgttgcgc atttgcgcag caatttgctc cagtacgcgc
    28321 gcacctgaaa cctgatgcgg caatgcgctg ataaccaccg cgccatcttc tttcttccaa
    28381 accgcgcgca tacgcactga accacgtccg ttctcgtaga ttttacgaat ctcggcgcgc
    28441 gaagtgataa tttccgcttc agtcggataa tccggcccct gcacgatatc cagcagctga
    28501 tcgagcgtgg ttttcggctg gtcgattaat gcgattgccg cctgagccac ttcacgcagg
    28561 ttatgcggtg gaatatcggt cgccatgccg acggcaatac cggtggtgcc gttaagcaaa
    28621 atgtttggca gacgggcagg cagcattttc ggctcctgca aagtgccgtc gaagtttggc
    28681 acccagtcag ccgtcccctg ccccagctcg ctcaatagca gctcggaata tttcgacaac
    28741 cgggattcgg tgtaacgcat tgccgcgaac gatttcggat cgtccggcgc gccccagttc
    28801 ccctgaccat caaccagcgg ataacggtaa gagaacggct gcgccatcag gaccatcgct
    28861 tcataacagg cactatcgcc gtgcggatgg tatttaccca gtacgtcacc gacggtacgg
    28921 gccgattttt taaatttggc gctggcattc aggcccagct cagacatcgc atacacaatg
    28981 cggcgctgaa caggtttcag accatcacca ataaacggca acgcacggtc catgatcacg
    29041 tacatggagt agtttaagta ggcgttttcc gtaaattcat gtagcgcaag gcgctctgcc
    29101 atatcgctca ttaattctga ttcctcaact tattcgccag ccttcaaagg ggcaatattg
    29161 ccggagatac tacattatct gccgcgtcga gtcacaggag atcagcgtga atagcgccgc
    29221 cggacgtgct ccggcggcga taaaggcatc aatgtgcctt gatatacaac tctttgctgt
    29281 agacataatc ctggggatta tgcagcggaa aaccgccaac gtagggttta agcagtttga
    29341 ttaacggctg atagtagatg ggaatcagcg gtgcctgctg gttgatgatc acttctgcct
    29401 gctgatacaa cgcattacgc tttgtcgcat cagtgatctg cgtggcctgg tttagtaagg
    29461 cgtcatactg cgcatttttc cagtgaccga cgttttcttc actatcgctt ttgagcgtgt
    29521 tcaggaagct ggaggcatca ttgtacgtcg catcccacga ctgccgagac agcataaaat
    29581 caccggctcg tcgggcatca agataggttt tccactccat tgtgcgcagc gtcacctgtg
    29641 cacccagcca ttttttccat tcggaagaca acgctatcgc ggtcttttca tgcagatcgt
    29701 acttgttgta gaacagctca aagcgtagcg gatgagaggc gtcgtatccc gcctgtttca
    29761 gcaaggcttt tgccatcgcg acacgctcac tcattggctt ttgcagttca tcgaacgtcg
    29821 tcgcgctaaa gccttttacc tctggcggcg tcagcgtggt tgcgggcgtt ctcaacccca
    29881 gtaccttttg cgcaataagc tgtcgatcaa ccgtaagata tagcgcccga cgcacccgca
    29941 catcgttaaa tggcggtttc tcaaggttaa agttgtaata ttcgctgttc agacgcggga
    30001 taattcgtag ctcgccaggc agtgattttt caatggcggg aatttgctgc gccggaaccc
    30061 aggtgagatc gacctctccc gcgcgatagc ggttatagcc ggtgaccgaa ttatccagcg
    30121 ccagatactc aacctgttgc aataccgtat gttgcgcatc gcggtacttt ggatttttgc
    30181 gtgcggtaat cttttcgtta actacccatt tatcaagcac aaaggcaccg ttgtaaacca
    30241 tgttctctgg cttactccag ctatcgccat gtttagcgat gacatgatga ggaaccggga
    30301 acaacgtcgg ccaggcgagc atcgtcgtaa accaaggaac tggctgctca agcgtaactt
    30361 caagagtacg atcatccgtc gctttgatcc ccagcgatgt aacatctgct ttacccgcaa
    30421 caatagccgc ggcattgtta atgtgcgcct gtgccagata gccagcaaaa gggcttgccg
    30481 ttttcgggtc aaccgcacgt tgccagccga ggacaaaatc ctctgccgtc agaggctgac
    30541 cgtctgacca ctgcaaaccg ctacgcagat ggaaaatata gcgcttgccg ccgtcaagta
    30601 tctcccagcg ttcagcctga gcgggctgca cctggccttc accgtccatc cataccagac
    30661 cttcaaacag atccagcaca atctgcgcgg cagtattctc ctctaccttt tgcgggtcga
    30721 gcgtgcctgg gtcgctatga ttgttgtaac gaaagacttg ttgcggggcg agcggtgtgt
    30781 tggcgggaac gtcagcagcg tagagaggag ccgcacttac caggctgacc agccataata
    30841 aatttcgcgt atacatggta ttccttaccg gagaaattat aagcccgaca acaatacgcc
    30901 tgtggcattg tttgctcaaa cttatcgcga gtgatttggc tcacactccg gtaaagattc
    30961 cccatcacgc caacggcaca taaatatccg tttccagtcg ccgctctgtc atcccttcgg
    31021 caagattggt gtagtgaaac aggatcgggg ctttgcgcat tttttcgccg cttgcaggta
    31081 gccagtggcg aatgatgccc catatcgtgt gtgaaatatc gtccagctcg ccaacatggc
    31141 gggcaacagc ataacgccca ccggtaagtt cgccattact cacgccataa cgattatcgg
    31201 ggatcggttc gctaacgctg ccgcagatat caaagcgaaa cgcttctggt gcggtggtgg
    31261 ctgggtcgtc ccaggcgacg ccaaaagtct ggctttggtt aacaggagaa agccccgtct
    31321 ctttacgcca catgataaat ttcgccacgc tgtagttgag caactcaggg ctacagcggt
    31381 gcggcaacat cgccacaggg attgatggga aatcgataat atttacatcc agtgtcaggt
    31441 ttgtcatttt gcctccctgc gaaattattc ccgggaacgc gtcccgggag cggtaacaat
    31501 tacggattta ctttgcggat ctgtttgacg tcaatttcaa cagagttcca gtctttatcg
    31561 acttccccct gaatctcaac cgtatctttc ggcgtcaccg tcacgccgtt ccagcgttta
    31621 tggtcgatat caacattgat agtaccgctg gcatctttga acacgtagag atcgtcagag
    31681 atgcgttcaa cgatattgcc gcgtagggtt acccaggtgt cgtcacgcag ggattttgcg
    31741 ctttctacag tcgttacgct gccgttcggc ccctggaatc ctccggcctg actttgcgtt
    31801 gccgatgggc cagaaaaacc tccctgctct gctgccataa ccggtgcact gcacagggcc
    31861 attactgcga ttactgctgc gaattttttc atgtttatta ctccctttaa tgtctgtttc
    31921 cgagcattta acaagatagt ccttaacaac ttcttaaggg aaaaaaataa aatttaatgc
    31981 tgtacagagc gcgttacaac acggtttact ggcagcaaat acggttatcg cagggatgaa
    32041 aaaatgcgaa ttttactgat agaagatgac atgctgattg gcgacggcat caaaacgggc
    32101 cttagtaaaa tgggttttag cgtcgactgg tttacacaag gtcgtcaggg aaaagaggcg
    32161 ctatatagcg caccttatga tgcggtgatc ctggatttaa ccttaccggg catggatggt
    32221 cgcgatattt tgcgcgaatg gcgagaaaaa ggtcagcgtg agccggtatt gatcctgact
    32281 gcgcgcgatg cgctggcgga acgtgtagaa gggctgcgtc tgggagctga cgattatctg
    32341 tgtaaacctt ttgcgttgat agaagtcgcc gccaggctgg aagctctgat gcgccgaacc
    32401 aacggccagg ccagcaacga gctgcgccac ggcaacgtca tgctagaccc cggcaaacgt
    32461 atcgccacgc tggctggcga ccccttaacg ctgaaaccaa aagaatttgc cctgctggaa
    32521 ttactgatgc gtaacgctgg tcgggtactg ccgcgcaaac tgattgaaga gaaactgtat
    32581 acctgggacg aagaggtcac cagtaatgcc gttgaagtgc atgtgcatca tctgcgacgc
    32641 aaactcggta gtgattttat tcgtaccgtg catggtattg gctacacatt aggtgagaaa
    32701 tgaaatttac ccaacgtctt agtctgcgag tcaggctgac gctaatcttt ttaattctgg
    32761 cctcggtgac ctggctgctt tccagctttg tcgcctggaa acaaacaacg gataacgtcg
    32821 atgagttgtt cgacacccaa ctgatgctgt ttgccaaacg gttaagtacg ctcgatctca
    32881 acgaaatcaa cgcggcggat cgcatggcac agacgccaaa taaattaaaa cacggtcatg
    32941 ttgatgacga tgcgctgact tttgccatct ttacccacga cggcagaatg gtccttaacg
    33001 atggcgataa cggagaagat attccttata gctatcagcg ggaaggtttt gctgacgggc
    33061 aactggtcgg tgaagacgat ccctggcgtt ttgtctggat gacctcacct gatggcaaat
    33121 atcgcatcgt tgtgggtcag gaatgggaat accgtgaaga catggcgctg gcgattgttg
    33181 ccggacaatt gatcccgtgg ctggtcgcac tgccaattat gttaatcatc atgatggtac
    33241 tactgggtcg tgaactcgcg ccgctgaaca aactggcgct ggcactacgt atgcgtgacc
    33301 ctgactcgga aaaaccacta aacgcgacgg gcgtacccag cgaagtgcgc ccactggttg
    33361 agtcgctaaa tcaactgttc gcccgcacac atacgatgat ggttcgtgaa cgacgcttta
    33421 cctccgacgc agctcacgaa cttcgtagcc cgttaacggc gctgaaagtg caaaccgaag
    33481 ttgctcagct ctctgacgat gatccgcagg cgcggaaaaa agcgctgctt caattacatt
    33541 ccggaatcga tcgtgctact cgtctggttg atcaactgct cacgctatcg cggctggact
    33601 cactggataa ccttcaggac gtcgcggaga tcccgcttga agatctcctg caatcgtcgg
    33661 tgatggatat ttaccacacg gcgcagcagg cgaacattga cgtgcgactg acactcaatg
    33721 ccaacggcat caaacgcacc ggacaaccgc tattgctaag tttgttggtg cgaaatttgc
    33781 tggataacgc cgtgcgctac agtccacagg gcagcgtggt ggacgtcacg ctgaatgccg
    33841 ataatttcat cgtgagggat aacggccccg gtgtgacacc agaggcactg gcgagaattg
    33901 gcgaacgctt ctatcgccca cccggacaaa ccgctaccgg cagtggactt gggctatcaa
    33961 ttgtccagcg aatcgccaag ctgcatgaca tgaatgttga atttgggaat gcggaacaag
    34021 gtggatttga ggcgaaagta agctggtaac ttctctgtct atgagagccg ttaaaacgac
    34081 tctcatagat tttattaata gcaaaatata aaccgtcccc aaaaaagcca ccaaccacaa
    34141 accaaacaaa taacgagcga gattacatat aaccaaaact caaacatttc tcgcacaaag
    34201 agttttctta agataataat aataagtggc gtcagaagaa aaaatgctgc aatcattgct
    34261 tgcaatatac gatcttcgcc tgaaacggaa ggtatactgc aaatatcatc tcccgactca
    34321 agcatccagt catacttatt
//
LOCUS       DAZUCC010000026        33542 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZUCC010000026 DAZUCC010000000
VERSION     DAZUCC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.026"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..328
                     /gene="ytcA"
                     /locus_tag="ACHY09_001121"
     CDS             53..328
                     /gene="ytcA"
                     /locus_tag="ACHY09_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFS3884123.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            347..1378
                     /gene="mdtN"
                     /locus_tag="ACHY09_001122"
     CDS             347..1378
                     /gene="mdtN"
                     /locus_tag="ACHY09_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFS3884124.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGIDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1378..3429
                     /gene="mdtO"
                     /locus_tag="ACHY09_001123"
     CDS             1378..3429
                     /gene="mdtO"
                     /locus_tag="ACHY09_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFS3884125.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            3426..4892
                     /gene="mdtP"
                     /locus_tag="ACHY09_001124"
     CDS             3426..4892
                     /gene="mdtP"
                     /locus_tag="ACHY09_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFS3884126.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPFYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTLRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQASPVVEKK"
     gene            5090..7237
                     /gene="fdhF"
                     /locus_tag="ACHY09_001125"
     CDS             5090..7237
                     /gene="fdhF"
                     /locus_tag="ACHY09_001125"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5507..5509,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFS3884127.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNKTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDTDQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7331..8020
                     /gene="yjcO"
                     /locus_tag="ACHY09_001126"
     CDS             7331..8020
                     /gene="yjcO"
                     /locus_tag="ACHY09_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFS3884128.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(8438..9751)
                     /gene="gltP"
                     /locus_tag="ACHY09_001127"
     CDS             complement(8438..9751)
                     /gene="gltP"
                     /locus_tag="ACHY09_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFS3884129.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(10093..10689)
                     /gene="nrfG"
                     /locus_tag="ACHY09_001128"
     CDS             complement(10093..10689)
                     /gene="nrfG"
                     /locus_tag="ACHY09_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFS3884130.1"
                     /translation="MKQSQIPVKMLTTLTILMAFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10686..11069)
                     /gene="nrfF"
                     /locus_tag="ACHY09_001129"
     CDS             complement(10686..11069)
                     /gene="nrfF"
                     /locus_tag="ACHY09_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFS3884131.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEAEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(11062..12780)
                     /gene="nrfE"
                     /locus_tag="ACHY09_001130"
     CDS             complement(11062..12780)
                     /gene="nrfE"
                     /locus_tag="ACHY09_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFS3884132.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(12800..13756)
                     /gene="nrfD"
                     /locus_tag="ACHY09_001131"
     CDS             complement(12800..13756)
                     /gene="nrfD"
                     /locus_tag="ACHY09_001131"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFS3884133.1"
                     /translation="MMQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTKPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13753..14424)
                     /gene="nrfC"
                     /locus_tag="ACHY09_001132"
     CDS             complement(13753..14424)
                     /gene="nrfC"
                     /locus_tag="ACHY09_001132"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFS3884134.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(14421..14987)
                     /gene="nrfB"
                     /locus_tag="ACHY09_001133"
     CDS             complement(14421..14987)
                     /gene="nrfB"
                     /locus_tag="ACHY09_001133"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFS3884135.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(15032..16468)
                     /gene="nrfA"
                     /locus_tag="ACHY09_001134"
     CDS             complement(15032..16468)
                     /gene="nrfA"
                     /locus_tag="ACHY09_001134"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFS3884136.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16861..18819
                     /gene="acs"
                     /locus_tag="ACHY09_001135"
     CDS             16861..18819
                     /gene="acs"
                     /locus_tag="ACHY09_001135"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFS3884137.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            19201..19515
                     /gene="yjcH"
                     /locus_tag="ACHY09_001136"
     CDS             19201..19515
                     /gene="yjcH"
                     /locus_tag="ACHY09_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFS3884138.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            19512..21161
                     /gene="actP"
                     /locus_tag="ACHY09_001137"
     CDS             19512..21161
                     /gene="actP"
                     /locus_tag="ACHY09_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFS3884139.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(21244..22893)
                     /gene="yjcE"
                     /locus_tag="ACHY09_001138"
     CDS             complement(21244..22893)
                     /gene="yjcE"
                     /locus_tag="ACHY09_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFS3884140.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23044..24393)
                     /gene="ghxP"
                     /locus_tag="ACHY09_001139"
                     /gene_synonym="yjcD"
     CDS             complement(23044..24393)
                     /gene="ghxP"
                     /locus_tag="ACHY09_001139"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFS3884141.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(24939..25403)
                     /gene="soxR"
                     /locus_tag="ACHY09_001140"
     CDS             complement(24939..25403)
                     /gene="soxR"
                     /locus_tag="ACHY09_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFS3884142.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            25489..25812
                     /gene="soxS"
                     /locus_tag="ACHY09_001141"
     CDS             25489..25812
                     /gene="soxS"
                     /locus_tag="ACHY09_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFS3884143.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(25815..27401)
                     /gene="pdeC"
                     /locus_tag="ACHY09_001142"
     CDS             complement(25815..27401)
                     /gene="pdeC"
                     /locus_tag="ACHY09_001142"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFS3884144.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGVYLATHVDRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            27831..28112
                     /gene="yjcB"
                     /locus_tag="ACHY09_001143"
     CDS             27831..28112
                     /gene="yjcB"
                     /locus_tag="ACHY09_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFS3884145.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(28212..28748)
                     /gene="ssb1"
                     /locus_tag="ACHY09_001144"
     CDS             complement(28212..28748)
                     /gene="ssb1"
                     /locus_tag="ACHY09_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFS3884146.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            29003..31825
                     /gene="uvrA"
                     /locus_tag="ACHY09_001145"
     CDS             29003..31825
                     /gene="uvrA"
                     /locus_tag="ACHY09_001145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS3884147.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(31860..32216)
                     /gene="yjbR"
                     /locus_tag="ACHY09_001146"
     CDS             complement(31860..32216)
                     /gene="yjbR"
                     /locus_tag="ACHY09_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS3884148.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(32220..32636)
                     /gene="yjbQ"
                     /locus_tag="ACHY09_001147"
     CDS             complement(32220..32636)
                     /gene="yjbQ"
                     /locus_tag="ACHY09_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFS3884149.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(32747..33460)
                     /gene="aphA"
                     /locus_tag="ACHY09_001148"
     CDS             complement(32747..33460)
                     /gene="aphA"
                     /locus_tag="ACHY09_001148"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFS3884150.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         8168 a         9111 c         8694 g         7569 t
ORIGIN      
        1 gatattgata ttcactacag atattgttcg cagcagacag gatgtattta atatgccaac
       61 agttctctct cgcatggcga tgcaactcaa aaaaacagcc tggataattc ccgtcttcat
      121 ggtttcggga tgctcattat ctccggcaat cccggtgatc ggcgcttatt atcccggctg
      181 gtttttctgc gccattgcca gccttatttt gacgctcatc acgaggcgaa ttattcagcg
      241 ggcaaatatc aatctggcat ttgtcggaat tatttatacc gccctttttg ctctctacgc
      301 catgctgttc tggctggcat ttttctaatc tatttgagac gcgctcatgg aaagtacgcc
      361 gaaaaaagct cctcgcagta aattccctgc tctgttagtg gttgcgttgg cgctggttgc
      421 ccttgttttc gttatctggc gcgtagacag tgcgccatca actaatgacg cttacgcgtc
      481 agcagatacc attgatgtgg tgccggaagt cagcggtcgc attgtagaac tggcggtcac
      541 cgacaaccag gcggtgaaac agggtgattt gctattccgc atcgacccac gcccatacga
      601 agccaatctg gcgaaagccg aagcctccct cgcggcgctg gataagcaaa ttatgctcac
      661 ccagcgtagc gttgacgcgc aacagtttgg tgccgactcg gttaatgcca cggtagaaaa
      721 agcccgtgcc gccgcgaaac aggccacaga tacattacgc cgcaccgagc cattactgaa
      781 agaaggtttt gtctcagcgg aagacgttga ccgtgcaaga actgcgcagc gcgccgcaga
      841 agcggatctt aatgccgtat tgttacaggc gcagtcagcc gccagcgccg tcagcggcat
      901 cgatgcgctg gtggcgcaac gtgcggcggt cgaagcggat attgccctga ccaaactgca
      961 tctggaaatg gcgaccgttc gcgcgccgtt tgatggccgg gtcatttccc tcaaaacctc
     1021 cgtcgggcaa tttgcttctg ccatgcgccc tatttttacc ctaatcgaca ctcgtcactg
     1081 gtacgtgatc gccaacttcc gcgaaaccga tctgaaaaat attcgctcag gtacacccgc
     1141 aaccattcgc ctgatgagcg acagcggcaa aaccttcgag ggtaaagtgg attcgattgg
     1201 ctacggcgtg ctaccggatg acggcggcct ggtgctgggc ggcctgccga aagtgtctcg
     1261 ttctattaac tgggtccgcg ttgcccagcg ttttccggtc aaaatcatgg tcgataaacc
     1321 tgacccggaa atgttccgca tcggcgcttc ggcagtcgct aatcttgagc cgcaataatg
     1381 agcgcgctca actccctgcc attaccggtg gtcaggctgc tggcgttctt tcatgaagag
     1441 ttaagcgagc ggcggccagg tcgcgtgccg cagaccgtgc aactctgggt aggctgcctg
     1501 ctggtaattc tgatctcgat gacctttgag atcccttttg tggcgttatc gctggcagtg
     1561 ctgttttacg gtattcagtc gaacgcgttt tacaccaaat ttgtcgcgat cttgtttgtg
     1621 gttgccacgg tgctggagat cggcagcctg tttttgatct acaaatggtc atacggcgaa
     1681 ccgttgatcc gattgatcat cgccggaccg atcctgatgg gctgtatgtt tttgatgcgc
     1741 acccatcggt tggggctggt ctttttcgcc gtcgccatag tcgctattta cgggcaaacc
     1801 ttccccgcca tgctcgacta tccggaagtg gtcgtgcgct taaccctgtg gtgtatcgtt
     1861 gtcggcctct atccaacctt gctgatgacg ttaatcggcg tgctgtggtt tcccagccgc
     1921 gctatcacgc agatgcatca ggcgcttaat gatcggcttg atgatgccat tagccacctg
     1981 acggacagcc tcgcaccgct acccgaaacg cggattgaaa gagaggcgct ggcgctgcaa
     2041 aaactcaatg tcttttgcct cgcggacgat gccaactggc gaactcagag cgcatggtgg
     2101 caaagctgcg tggcaacggt aacctacatt tactcgacgc tgaatcgcta cgatcccacc
     2161 tcttttgctg attctcaggc aattattgaa tttcggcaaa aattagcctc agaaatcaac
     2221 aagctgcagc atgccattgc cgaaggtcag tgctggcaaa gcgactggcg gatcactgaa
     2281 agtgaagcga tggcggcacg ggaatgtaac ctggagaata tctgccagac gttattgcaa
     2341 ctgggtcaga tggacccgaa tacgccgcca acgcccgcca ccaaaccgcc atcaatggtc
     2401 gccgacgctt ttaccaatcc agactatatg cgctacgcgg taaaaacgct gctcgcctgt
     2461 ttgatctgtt acaccttcta cagcggcgtg gactgggaag gcattcacac ctgtatgctg
     2521 acctgcgtga tcgtcgctaa tcccaatgtc ggttcgtcgt accagaagat ggtgctgcgt
     2581 tttggcgggg ccttttgcgg cgcgattctg gcgctgttat tcacgttatt ggtcatgccc
     2641 tggctggaca atattgtcga attgctgttt gtgctggcac cgattttcct gttgggcgca
     2701 tggattgcca ccagctctga acgctcttct tatatcggca cacagatggt ggtcaccttc
     2761 gcgctcgcca cgctcgaaaa cgtttttggc ccggtgtacg acctggtgga aattcgcgat
     2821 cgcgctctgg gtatcatcat tggtaccgtg gtgtcggcgg tgatttacac ctttgtctgg
     2881 cctgaaagcg aagcgcgcac gctgccgcaa aaactggctg gcgcgctggg tatgttgagt
     2941 aaagtaatgc ggatcccacg ccagcaggaa gtcacagctc tgcgcactta tctgcaaatt
     3001 cgcataggtc tgcatgcggc gtttaatgcc tgtgaagaga tgtgccaacg cgtggcgctg
     3061 gagcgtcaac tggacagcga agaacgcgct ttactgattg aacgttcgca aacggttatt
     3121 cgtcagggcc gcgatattct tcacgcctgg gatgcaacct ggaactcggc gcaggcgctg
     3181 gataacgcac tacagccgga cagagcaggt cagtttgccg acgcactgga gaaatacgcc
     3241 gccggtctgg caaccgcact cagtcgttct cctcaaataa cgcttgaaga aacacccgcc
     3301 tcgcaggcca tcctgccgac cttattaaaa caggagcaac acgtctgcca gcttttcgcc
     3361 cgcttgccag actggacagc cccggcatta acgcccgcca cggaacaggt acaaggagcc
     3421 acgcaatgat caatcgtcaa ctttcacgcc tgctgttgtg cagcattctc ggcagcacga
     3481 cgctgatttc cggctgtgcc ctggtacgta aggattctgc gcctcatcaa cagctcaaac
     3541 cggaacaaat caaactggcc gatgatattc atcttgccag ctccggctgg ccgcaggcgc
     3601 agtggtggaa acaactcaat gacccgcagt tggatgccct gatccaacgg acgctaagtg
     3661 gttcacacac cctcgccgaa gcgaaactgc gggaagaaaa agcgcagtcg caggccgatt
     3721 tgttagatgc cggttcacaa ttacaggtcg cagcgttagg gatgctcaac cgccaacgcg
     3781 tctcggcgaa cggcttttta agcccttatg cgatggatgc gccagcactg ggtatggacg
     3841 ggccgttcta tacggaagcc acagtcggtt tgtttgccgg actggatctc gatttgtggg
     3901 gtgtgcatcg ctcagcggtt gctgccgcca ttggcgcgca taatgccgcg ctggcagaaa
     3961 ccgcagcagt agagctatcg ctgaccacgg gcgtagcgca gctttattac agtatgcagg
     4021 ccagctatca gatgctcgat ctgttagaac aaacacgcga tgtgattgat tacgcggtga
     4081 aagcgcacca gagtaaagtg gcgcacggtc tggaagcgca agtgcctttc cacggcgcgc
     4141 gggcacagat tctggcggtc gataaacaaa ttgttgccgt taaagggcaa atcaccgaaa
     4201 cgcgagaatc tctgcgtgca ttgattggcg cgggagccag cgatatgccg gagatcaaac
     4261 cggtggcatt accgcaagtc cagaccggca ttccggcgac actctcttat gagttgctcg
     4321 ccagacgccc ggatctgcaa gcgatgcgct ggtatgttca ggcgtcatta gatcaggtgg
     4381 attccgcgcg ggcgctgttc tatccgagct ttgatatcaa agcgtttttc ggcctggact
     4441 ctatccacct ggatacctta ttcaaaaaaa ccagtcgcca gttcaacttt atcccgggtc
     4501 tgaaattgcc gctgtttgac ggtggacggt tgaatgccaa tctcgaaggc actcgcgccg
     4561 ccagcaacat gatgattgaa cgttacaacc agtcagtact gaatgcggtg cgcgacgttg
     4621 ccgtcaacgg cacgcgtctg caaacgctca acgacgagcg agagatgcag gctgaacgcg
     4681 tggaagcaac gcgctttacc ctgcgcgctg ccgaggccgc ctatcagcgc ggcttaacca
     4741 gccgcttaca ggccaccgaa gcccggttgc cagtacttgc cgaagagatg tcattactga
     4801 tgctggacag ccgccgggtg atccaaagca ttcagttgat gaaatcgctg ggcggcgggt
     4861 atcaggcaag tcccgtcgtc gagaaaaaat aaaatgtctg ccgcgtgatg gctgtcacgc
     4921 ggtatttcgt ttcgtcacgt caaaactgac gacagcctgt ttttcgtcag agttttgaat
     4981 aaatagtgcc cgtaatatca gggaatgacc acaaataaaa tgtggcataa aagatgcata
     5041 ctgtagtcga gagcgcgtat gcgtgatttg attaactgga gcgagaccga tgaaaaaagt
     5101 cgtcacggtt tgcccctatt gcgcatcagg ttgcaaaatc aacctggtcg tcgataacgg
     5161 caaaatcgtc cgggcggagg cagcgcaggg gaaaaccaac cagggtaccc tgtgtctgaa
     5221 gggttattat ggctgggatt tcattaacga tacccagatc ctgaccccgc gcctgaaaac
     5281 ccccatgatc cgtcgccagc gtggcggcaa actcgaacct gtttcctggg atgaggcact
     5341 gaattacgtt gccgagcgcc tgagcgccat caaagagaag tacggtccgg atgccatcca
     5401 gacgaccggc tcctcgcgtg gtacgggtaa cgaaaccaac tatgtaatgc aaaaatttgc
     5461 gcgcgccgtt attggtacca ataacgttga ctgctgcgct cgtgtctgac acggcccatc
     5521 ggttgcaggt ctgcaccaat cggtcggtaa tggcgcaatg agcaatgcta ttaacgaaat
     5581 tgataatacc gatttagtgt tcgttttcgg gtacaacccg gcggattccc acccaatcgt
     5641 ggcgaaccac gtaattaacg ctaaacgtaa cggggcgaaa attatcgtct gcgatccgcg
     5701 caaaattgaa accgcgcgca ttgctgacat gcacattgca ctgaaaaacg gctcgaacat
     5761 cgcgctgttg aatgcgatgg gccatgtcat tattgaagaa aacctgtacg acaaagcttt
     5821 cgtcgcttcc cgtacagaag gctttgaaga gtatcgtaaa atcgttgaag gctacacgcc
     5881 ggagtcggtt gaagatatca ccggcgtcag cgccagtgag attcgtcagg cggcgcggat
     5941 gtatgcccag gcgaaaagcg ccgccatcct gtggggcatg ggtgtaaccc agttctacca
     6001 gggcgtggaa accgtgcgtt ctctgaccag cctcgcgatg ctgactggta acctcggtaa
     6061 gcctcatgcg ggtgtaaacc cggttcgtgg tcagaacaac gtgcagggtg cctgcgatat
     6121 gggcgcgctg ccggatacgt atccgggata ccagtacgtg aaagatccgg ctaaccgcga
     6181 gaaattcgcc aaagcctggg gcgtggaaag cctgcctgct cataccggtt atcgcatcag
     6241 cgagctgccg caccgcgcag cgcatggtga agtgcgtgcc gcgtacatta tgggcgaaga
     6301 tccgctgcaa actgacgcgg aactgtcggc agtacgtaaa gcctttgaag atctggaact
     6361 ggttatcgtt caggacatct ttatgaccaa aaccgcgtcg gcggcggatg tcattttacc
     6421 gtcaacgtcg tggggcgagc atgaaggcgt gtttactgcg gctgaccgtg gcttccagcg
     6481 tttcttcaag gcggttgagc cgaagtggga tctgaaaacg gactggcaga ttatcagtga
     6541 aatcgccacc cgtatgggtt atccgatgca ctacaacaaa acccaggaga tctgggatga
     6601 gttgcgtcat ctgtgcccgg atttctacgg tgcgacttac gaaaaaatgg gcgaactggg
     6661 cttcattcag tggccttgcc gcgatacttc agataccgat caggggactt cttatctgtt
     6721 taaagagaag tttgataccc cgaacggtct ggcgcagttc ttcacctgcg actgggtagc
     6781 gccaatcgac aaactcaccg acgagtaccc gatggtactg tcaacggtgc gtgaagttgg
     6841 tcactactct tgccgttcga tgaccggtaa ctgtgcggca ctggcggcgc tggctgatga
     6901 acctggctac gcacaaatca ataccgaaga cgccaaacgt ctgggtattg aagatgaggc
     6961 attggtttgg gtgcactcgc gtaaaggcaa aattatcacc cgtgcgcagg tcagcgatcg
     7021 tccgaacaaa ggggcgattt acatgaccta ccagtggtgg attggtgcct gtaacgagct
     7081 ggttaccgaa aacttaagcc cgattacgaa aacgccggaa tacaaatact gcgccgtgcg
     7141 cgtcgagccg atcgccgatc agcgcgccgc cgagcagtac gtgattgacg agtacaacaa
     7201 gttgaaaact cgcctgcgcg aagcggcact ggcgtaatac agtcctttct acagcctcct
     7261 ttcggaggct gtttttttat ccattcgaac tctttatact ggttactccc tacccaatcg
     7321 tattatcaaa atgaaaaaaa ttatcgcatt gatgttgttt ttgacattct ttgcccacgc
     7381 gaacgactcc gagcctggca gccagtattt aaaggcagca gaggcagggg accgacgcgc
     7441 acaatatttt cttgccgaca gctggtttag ctccggcgat ttgagcaaag ccgaatattg
     7501 ggcacagaaa gccgccgaca gcggtgatgc tgatgcctgc gcgctgttag cgcagatcaa
     7561 aatcaccaat ccggtcagtc tggactatcc acaagcaaaa gttcttgcag agaaagcggc
     7621 gcaagcgggc agtaaagaag gtgaagtaac gctggcgcat attctggtaa atacgcaagc
     7681 gggtaaaccg gattatccaa aggcaatttc gctgttagaa aacgcctcgg aagatctgga
     7741 gaacgactct gccgtcgatg cccaaatgct gcttggtttg atttacgcca acggcgtggg
     7801 cattaaggcc gacgatgaca aggcaacctg gtatttcaaa cgcagctctg cgatttcccg
     7861 aaccggttat tccgagtact gggcgggaat gatgttctta aacggtgaag aaggttttat
     7921 cgagaagaac aaacaaaagg cgctgcactg gttaaatctg agctgtatgg aagggtttga
     7981 taccgggtgt gaagagtttg aaaaattaac taacggttaa ggtaggcctg ataagacgcg
     8041 gtaagcgtcg catcaggcat tgatgtcgga tgcggcgtaa acgccttatc cgacctacaa
     8101 aatcacgcta aatcaggcta ttgcgcagtc tggcgtaggc ctgataagac gcggtaagcg
     8161 tcgcatcaga tattgatgtc ggatgcggcg taaacgcctt atccgaccta caaaatcacc
     8221 ctaaatcaag ctattgcgca gtctggcgta ggcctgataa gacgcggcaa gcgtcgcatc
     8281 agatattgat gtcggatgcg gcgtaaacgc cttatccgac ctacaaaatc accctaaatc
     8341 aagctattgc gcagtctggc gtaggcctga taagacgcgg taagcgtcgc atcaggcatc
     8401 cacatattgc cgggtggata tccccggcaa tcttcaatta ttgatccgca gttttatcaa
     8461 atttgcccag cacttcgcgc tcataagcca gcgctttctt acggtcaaat ttgtgttccc
     8521 acttggcaat caccagcacc gccagcgcat tacccaccac gttcagcgca gtacgcgcca
     8581 tgtcgaggat acggtcaaca ccggcaataa acgccagacc ttccagcgga atacctacgc
     8641 tacccagcgt tgccagcaac accacaaacg acacgcccgg cacgccagca atccctttcg
     8701 aggtcaccat cagcgtcagc accagaatga tttcctgcca gatggacagg tcaatgccat
     8761 acaactgcgc gatgaaaata gcggcaatac tttgatacag cgtcgaacca tcaaggttaa
     8821 aagagtaacc ggtcggcacc acgaaactgg tgatcgacgc cggtgctccg taggcttcca
     8881 tcttctcaat aattcgcggc agcacgcttt cagagctggc agtggagtac gccagaatca
     8941 gctcatcttt cagaatacga atcaggatcc agacgcttaa cccgcacagg cgcgccacaa
     9001 ttcccagcac taccagcgcg aagaacagaa tggcgaaatg caccagcagc accagtttcg
     9061 ccagtggcca cagagacgag aaaccaaagt tagccaccgt caccgcaatc agcgcaaaca
     9121 cacccaccgg cgcataacgc atcaccatgt gagtcacttt aaacatggtt tcagagatgg
     9181 agcggaaaac ggtcaccagc ggttcacgat gcgtcgcggg cagggatgaa agccccagac
     9241 caaacagcac cgagaagaag atgatcggca gcatttcgcc tttcgccatc gacgccacaa
     9301 tgttcgtcgg caccagcgac aaaatcgtgc ccataatgcc gtgggaactg ctttgtaccg
     9361 cttccgtagt gctctgatat ttcgagatat cgacggtcgc cagctgcgac atatccaccc
     9421 cggcaccggg ctggaagacg ttcgccagag tgatccccaa aatgatggcg acggtggtga
     9481 tcacctcgaa gtaaataatg gttttcgcgc caatacgccc gagctgtttg gcatcaccaa
     9541 cacccgcgat acccaccacc agcgtggaga tcacaatcgg cacaacaatc attttgatca
     9601 gatggatgaa gatatcaccc gccggagaga gcaaattgac gaccagccag tcgcggctgt
     9661 cgctatggta gtgcaggtag cttcccagga gaatgcccag caccatagca aacagaatct
     9721 gccaggccag gctgaatttt atatttttca tcagaataac ttcctcaatg gagcgccgtt
     9781 ccccgttcag aaacgcgaga acgtgacata ctgccagggc atgaattgtg ttgcgttggt
     9841 ttgtagattt ttttaacgct tatgatcagt atcatttgcg cggtatgttg cgcaaccctt
     9901 taacaacaga gcttttcgct gcattaatgc ataataacgc gagtttatgg caggattaat
     9961 gaataaccat ttgttatgga aagattttta tttgtttaat ctgcaataat actgattggt
    10021 gattattttt ggcatatgtt cattcgatta ttttttatgc agctcaataa cataatcttc
    10081 atcaccagat gattaatctg atctccgctg caacaatttc gccatattaa tcgactcgac
    10141 cagctgtgtc cggttgatcc ggggtgagtt gagatccatc actttttgcc atagttcgat
    10201 ggcctgcgcg taattcgcct gcataaacgc atcggaagcc agcagcatca gagcggtgat
    10261 ttcgttagag tccagcgcga gggctttatc gatcattgcg cgtgtctggg cggtcatatg
    10321 ctggctggcc tggtaataaa gcaccgtcgc cagcgccgca tacagttcag cgttctcacc
    10381 acgtagttgc aacgcctgac ggtacgccag cagcgaattg ctgtaatcat tttgccacag
    10441 atagtactcg cccagtaacg cccactgttc gctgttttgt ggattagcgc ggattttatc
    10501 ctgcaatgcc tgaagctgcg cttctggggt ttgctggctg gcaaactgat gcagcggatc
    10561 gcgctgacgc tgatactccg cacgtaccgc ctgccatttt gggcttaaca gataactgcc
    10621 gatacagaga aatgccatca aaatcgtgag tgttgtcagc attttcaccg gtatttgtga
    10681 ctgtttcatc gcttcgccct cactcgccag aggatcagtg ccatcagcag taacaacacc
    10741 actggcagcg cccacaacac cagcgtctgg cccgttaacg gtgggttata gcgaacaaaa
    10801 tctccgtagc gttcggtcat ccagccgatg atttcagctt cgttcttccc ctccgccacc
    10861 atgctgtaaa cctgatggcg catactgacc gccaccggcg cgttggattc cagtaagttt
    10921 tgattctggc actgcggaca acgtaactgg ctggcaatat ttaacgcctg ttgctgttgt
    10981 tgcggattgg cgaattgcca ggtgtctacg acctgagcgc gcgcaaaaca ggtaaataac
    11041 agcagtagcg tgagaagtcc tttattcatc gcgcttcctc ccccgccatc cgcttaacaa
    11101 tgcgcccgca atcatcaaca atcctccccc ccagatccag cgcacaccgc tttgtacata
    11161 caaacgaaaa gcgtaacgat ccgctccggt tttctcgccc atgaccgcat accagtcatg
    11221 gatgccgttc cagcgaattg acggttccat catttgctga cggcgtgctt cataaaaacg
    11281 ccgctccggc gtcagttcac caatgcgttg ctgatggtca aacagcgcca ctatcgcttt
    11341 ttcgctggtg taattgcctc tggcttgcag atcgagtcgc tcaaaacgga aggtgtatcc
    11401 tgccagcgtc acctgctgac ccggctgtaa attcaggctg atctcctgac gactgacgct
    11461 ggaaaccacg atccccgcgg caaataacag cacgccagca tgagctacca gcgccggaag
    11521 ctgcacgcgt ttgccagaga caaacgtcgc cagcacaatc accaccagca tcaacagacc
    11581 aaacggtaac gtcgcgcggt taaaatacgg cgcgccaacg gagaggcgtc cccagcccag
    11641 cagaccgtaa atcatcggat aaagcgttcc caccagcacg atcagtagca ctgcgcaaaa
    11701 cagcaacagc gtagcgagga ttaacatttc ccgcgataac cccgaaaaac gcaccgccgg
    11761 gccaccgtcc cgcgctcgcc agccatacag agccagagac gcaaggctaa tcagtgcaaa
    11821 caggctgaac aacggcacgg cgcggacgtt atccagtgcg aacgcatgaa ccgaaaccag
    11881 aatgccagaa cggacaatta aggtgcccag cagcgacagc atcagagtaa ctatcgccag
    11941 cagcagcgac cagtggcgga aaatcccccg ctggcgtgtc agggacaaac tgtgcagcag
    12001 cgcagtggca gaaagccagg gtaataaaga ggcgttttcc accggatccc agaaccacca
    12061 gccgccccag cccagttcgc aataggccca ccaggaaccg aggatgatcc cagccgttaa
    12121 tgcactccag ccaggtaacg cccagcgcca gcaaattcgg gcgcaggcac catcaaactc
    12181 gccgcgcagt aaactcgcca gcgccacgct cgccgccacc atcaaaccgc catagccaag
    12241 gtaaagcagc ggtggatgaa agataagacc gggatgttgc agcatcggat tgagatcgcg
    12301 gccttcgatt gctggtggaa atatccgcac gaagggatcg gaccacagca ctacaaacag
    12361 tagcagtgcg gcgagcatga gagataaaac ggctagcgtt agcggaaata gcggatcggt
    12421 ttgctgccga taatgccagg caaacagcgc gctccaggcg gaaagcagca gcacccaaag
    12481 cagcagcgaa ccttcatgac cgccccacac cgccgccagt ttgagttccc acgacagcag
    12541 gctgtagcta tgttgggcga cataaatgac cgagaaatcg ctgatgagaa aacaatacgt
    12601 cagtacgcca aaggcgagca gcaggagcgc gaactgcgcc agaatgccga tgcaagtgag
    12661 tcgcatcatg gcaggccagc gcaaccgcac tcccgcgaag gccgtcaacg gggtcaacac
    12721 gttgaccccg agacttaaca acaacgccag aaaaccgact tcaggaagga atacgtccag
    12781 aaaccacctc ttttctggct tacgccaccg tcaactgtcc ggcgtagaga atgaaaaagc
    12841 gcagcatcaa cacgccgacc agactggccc cacacgccgc cagcacggta ggaatgccgg
    12901 aactgcgatt gacccacggt ttgagcaaca ttggcacaat cagccccagc ccggcgacac
    12961 caagccagaa ccaccacgtc cagaaaccgc cacctaatgc cgccaccagc gcacgcactt
    13021 taccgtcatc gcccagcgcc agaccgacaa aaaacgccac cagcaggaag atttcacccc
    13081 ataccacagg gatttccata cggtgaacaa agtgcgcttc cgtggaatgc ggatttttgc
    13141 gttggcgtat cgccatggcg atcagcgcta ccgccgcacc ggacgagatg ccggagaaga
    13201 ggaacagcac cggcaggatc gggttattga ggaacggata cgatttcagc gccgacagca
    13261 gaaagccggt ataagccccc agcagcaccg ccagcaccag catcaatgtt tccagtccgc
    13321 gatgaacggg cgtcagcagg ctcagaacct tttgcacgat ccccagcttc ggcaaccagc
    13381 gttgttgcag ggcaagcaaa tcatgttcaa agattttcgc cagccacagc accagcacca
    13441 ccatgtagag ctgaaacaac atcaccccca tcgacatcac cgaggtaaaa ctgtagtgga
    13501 acatcagctt ccagaaggtc cacggctttg tcaggtggaa gacgaggatc aacagaccga
    13561 ggatcaccgc gccaggcccg acaattagcg tggtgcgcag caacgtactg tctgcaccgc
    13621 ccgcctgcgg gtagaagcga cgtaacagca cggccagcgt caccagaccg gcagaaatac
    13681 caatcaaaaa caggtagatg gcaatcggcc agtcccacac cagcgattca aaatgaaatg
    13741 cggaagtctg catcattggc tcacctcccc gtatttaaac ggtacgcggt acagcttcgg
    13801 tttggttccc agcgccagct tgtagcggta agtgggcttc tggcgcagca gttgcgaaat
    13861 ctcactgttg ggatcgtcca gattgccaaa cgtcagtgcc ttggtcgggc aagcttcaac
    13921 gcacgcgggc agcttaccgg cctgcaaatt ggtcttacgg cagaaatcgc atttgtccgc
    13981 cgtcttcgtg accggatgga taaagcgcac gcggtacgga caggcggcga tgcagtactg
    14041 acaaccgacg cagagatcag gattaacatc gacaatcccg ctagcggcat cacgaaaaga
    14101 cgcaccggtc gggcagacgt caacgcacgg cgcatgatcg cagtgctggc aagacttacg
    14161 gaagaagcga tatttcacat caggaaattc gccctgcggc tcgctacgaa taatcgtcag
    14221 gcgcgagacg ccttccggca ccttgttcac ttcccgacaa gcatccatac aggcggtaca
    14281 gccgatgcat aacgactcgt catgcaccat gccgtaacgc accccattga tattcaacgt
    14341 tttcgccacc acgcgccctg cggtgccgct gacggctgcc agcacgccga cgccggtgag
    14401 aaactggcga cgagaccagg tcatggctgc tccttaagca acggaacaga cgccgggtta
    14461 aagttcggat tggtgcgctg atcgctgtgg caatcgacgc aaatcttaat ccgtcctttg
    14521 tcacttaacg tctgcatcgt atcttgctgt ggatgcaggg aatggcagct ggcgcacgcc
    14581 actttggtta cgtggacatc gtgtggccag aacgcttttt gcaactgttc cggcagatgg
    14641 caggacatac agacgctgtt ctgctctcca accttgtaca tcggctcgtt aaagcgcatc
    14701 acatctttca ccccttcgcg gtgttgcggt gatggctgac cgtggcagtt ggtgcaggtg
    14761 accggcagtt tgttattcgg gttgataacg gaagcatgtt tgccatgcat accttcggta
    14821 tctggcttat gacagtccag acaggcggca tccgggttac gctgctgggt aacttcgtag
    14881 cgatcaccag atgcctgcgc cgcaggggta gcggtaattc cgttcaggct ccacaacagg
    14941 cccgacgcca gcaccccggc agttaataac gaacgtaata cgctcatatt cactccattg
    15001 tgttttccac cccttgcgag gcggaacggg gttattggct taacagaccg tttttacgtg
    15061 cctgctcttc ccactgcggg atcaccgttt tgatgaagtc ctgcttctcg gccttgattt
    15121 gctccatgtt caggccaatg gcctgctggg ctttctcttt ggtcgagata tccgggatct
    15181 ggatttcatg ggtaatgcct ttggtcgcca gcaggcgcgc cagtttggtg cgtgcatccg
    15241 ccgctttatc catcgccgta ccgagcatcc tcagaccttc ttccggtgcg tgcatatgaa
    15301 tgccgtggga agcgatcgcc aaatcccaac gccactgggc atgacggata tcgtcctgaa
    15361 tcggcttcat ttcggcttcc gttgcgcctg catccagcgc cgctttcgct tcgaagtgag
    15421 cgtgaaccag ttgatcttca accttgattt tcaggtcgtt aatcgactgc ttacgttccg
    15481 cgaccacttt ttgcaaggca gctttgtcct gggtatggca gttcgcacaa gtctgggcga
    15541 agttatcaaa cggattacca attttatggt cggtgtagag tttgccttcg gcgttctgca
    15601 cttttggcat atggcaatcg atacaggtca cgttgttttt gccgtgaatg cccgctgtcc
    15661 aggtttcata ttccgggtgc tgcgctttca gcattggcgt tttcgacagg gagttagtcc
    15721 agtcagagaa ggcaattttg tcgtaatact gctccatatt ttcgactttc atgccgtcat
    15781 cccacgggaa tttaaccgct ttgtttttgc cgtcgaagta atactccaca tggcactgac
    15841 cgcaaaccat cgattgctgg tcgaaacgtc cggctttctc aaaaggttta ccaatggctt
    15901 ccatcgcgcg agccgcatac ggacgggaaa gggttaactc cggtttgcct ttggcgaact
    15961 ctggtgaggc ggtgttatgg caatcggcac aacctaagtt gttgacgatt tccgggccgc
    16021 cgcgcgccca ttttccgtgg aagtagccat cttcgccgtc tttctggatc agacgcgcca
    16081 catctgggct tttacaactc cagcatgcca tcggtagcgg accatcttca gcgtttttcg
    16141 gcgcaccggt acgcagggtt tcacgcacat cggtcacagc aaaggcatgt ccacgcggct
    16201 tgttgtaatc gcgcgagaag ggataccccg cccacaggat caccagccgt ggatcttccg
    16261 ccagggcgtc aacacgctct gactgttccg aggtggcttt ccaggagaga tattgatcgg
    16321 gatgttgcgg ggcaaaggtt tcattcttcg cttcaacagt tacaggtttt gcaggagctg
    16381 ccgtttgttc agcgtgaaca gaagtgaaaa agaaaaaagg aatcaataag ctgaagatac
    16441 ggcgtgcgtt tatttttatc cttgtcatag gggcttcatc cgaattgcgc cattgttgca
    16501 atggcggttt ttattgtttt tcacgacagt aaccgcacct acactgtcat gacattgctc
    16561 gcccctatgt gtaacaaata accacactgt gaatgttgtc tttaatcaat tgtaagtgca
    16621 tgtaaaatac cactttagag ttagtcagta tcttcctctt tttcaacagc atgcataact
    16681 gcatgttcct caaagaatta atcaactttt gttgctgacc ttcaaaaatt accctgccgt
    16741 ttatttgcac aattctactt ttgcgtgatc tgtcgcccaa atactaaaca aaactgccaa
    16801 tacccctaca tttaacgctt atgccacata ttattaacat cctacaagga gaacaaaagc
    16861 atgagtcaaa ttcacaaaca caccattcct gccaacatcg cagaccgttg cctgataaac
    16921 cctcagcagt acgaggcgat gtatcaacaa tctattaacg cacctgatac cttctggggc
    16981 gaacagggaa aaattctcga ttggatcaaa ccttaccaga aggtgaaaaa cacctccttt
    17041 gcccccggta atgtgtccat taaatggtac gaggacggca ctctgaatct ggcggcaaac
    17101 tgccttgacc gccatctgca agaaaacggc gatcgtaccg ccatcatctg ggaaggcgac
    17161 gacgccagcc agagcaaaca tatcagctat aaagagctgc accgcgacgt ctgccgcttc
    17221 gccaataccc tgctcgagct gggcattaaa aaaggtgatg tggtggcgat ttatatgccg
    17281 atggtgccgg aagccgcggt cgcgatgctg gcctgcgccc gcattggcgc ggtgcattcg
    17341 gtaattttcg gcggcttctc gccggaagcc gttgccggac gcattatcga ttctaactca
    17401 cgactggtga tcacttccga cgaaggcgtg cgtgccgggc gtagtattcc gctgaagaaa
    17461 aacgttgatg acgcgctgaa aaacccgaac gtcaccagca ttgaacatgt ggtggtactg
    17521 aagcgtactg gcgggaaaat tgactggcag gaagggcgcg acctgtggtg gcacgacctg
    17581 gttgagcaag ccagcgatca gcaccaggcg gaagagatga acgccgaaga tccgctgttt
    17641 attctctaca cctccggttc taccgggaag ccgaaaggcg tgctgcacac taccggcggt
    17701 tatctggtgt acgcggcgct gacctttaaa tatgtctttg attatcatcc gggtgatatc
    17761 tactggtgca ccgccgatgt gggctgggtt accggacaca gttatttgct gtacggcccg
    17821 ctggcctgcg gcgcgaccac gctgatgttt gaaggcgtac cgaactggcc gacgcctgcc
    17881 cgtatggcgc aggtggtgga caagcatcag gtcaatattc tctataccgc gcccacggcg
    17941 atccgcgcgc tgatggcgga aggcgataaa gcgatcgaag gcactgaccg ttcgtcgctg
    18001 cgcattctcg gttccgtagg cgagccaatc aacccggaag cgtgggagtg gtactggaaa
    18061 aaaatcggca acgagaaatg cccggtggtc gatacctggt ggcagaccga aaccggcggt
    18121 ttcatgatca ccccgctgcc tggcgctacc gagctgaaag ccggttcggc aacacgtccg
    18181 ttcttcggcg tgcaaccggc gctggtcgat aacgaaggta acccgctgga aggggccacc
    18241 gaaggcagtc tggtgatcac cgactcctgg ccgggtcagg cgcgtacgct gtttggcgat
    18301 cacgaacgtt ttgagcagac ctacttctcc accttcaaaa atatgtattt cagcggcgac
    18361 ggcgcgcgtc gcgatgaaga tggctattac tggatcaccg ggcgcgtgga cgacgtgctg
    18421 aacgtctcgg gtcaccgtct gggaacggcg gagattgagt cggcgctggt ggcgcatccg
    18481 aaaatcgccg aagccgctgt cgtcggtatt ccgcacaata ttaaaggtca ggcgatctac
    18541 gcctacgtca cgcttaatca tggggaggaa ccgtcaccag aactgtacgc agaagtccgc
    18601 aactgggtgc gtaaagagat tggcccgctg gcgacgccag acgtgctgca ctggaccgac
    18661 tccctgccta aaacccgctc cggcaaaatt atgcgccgta ttctgcgcaa aattgcggcg
    18721 ggcgatacca gcaacctggg cgatacctcg acgcttgccg atcctggcgt agtcgagaag
    18781 ctgcttgaag agaagcaggc tatcgcgatg ccatcgtaac ccacaattgc ccgatgcgag
    18841 tcggtaacgg tttgtaggcc tgataagacg cgacagcgtc gcatcaggcg ctggttgccg
    18901 gatgcggcgt gaacgcctta tccggcctac attcggcaac ggctgtaggc ctgataagac
    18961 gcgacagcga cgcatcaggc gttgattgcc ggatgcggcg taaacgcctt atccggccta
    19021 cattcggcaa cggctgtagg cctgataaga cgcgacagcg tcgcatcagg cgttgattgc
    19081 cggatgcggc gtgaatgcct tatccggcct acattcggca agggttaccc aagcgttaac
    19141 cttctcccat aagggagcgg gaattaaaac aatccctaca ttacctctgg agaatctgtg
    19201 atgaatggta cgatttatca gcggatagaa gacaatgcgc atttcaggga gttagtcgaa
    19261 aaacggcaac ggtttgccac catcctgtcg attattatgc tggcagttta tatcggcttt
    19321 attttactga tcgccttcgc gcccggctgg ctgggcaccc cgctgaatcc gaacaccagc
    19381 gtcacacgcg gtattccgat tggtgttgga gtaattgtga tctcctttgt tctcactggt
    19441 atctacatct ggcgggcgaa cggcgaattc gaccgtctta ataatgaagt cctgcatgag
    19501 gtacaagcat catgaaaaga gttctgacgg cgcttgccgc cacactccct ttcgcagcta
    19561 acgccgcgga tgctattagc ggggccgtag agcgccagcc aacaaactgg caggcgatta
    19621 ttatgttcct gattttcgtc gtgtttacgc tcggcattac ctactgggcg tcaaaacgcg
    19681 tacgttcgcg taatgactac tacaccgcag gcggcaatat caccggtttt cagaatgggc
    19741 tggcgattgc cggggactat atgtccgccg cctcattctt ggggatctcc gcgctggtgt
    19801 ttacctccgg ctatgacggc ttaatttact cgctgggctt cctggtaggc tggccaatca
    19861 ttctgttcct gattgccgaa cgtctgcgta acctgggccg ctacaccttt gccgatgtag
    19921 cctcttaccg cctgaaacaa gggccgattc gtattctttc ggcctgtggt tctctggtgg
    19981 tggtggcgct ttaccttatc gcccagatgg tgggcgcagg taaactcatc gagctgctgt
    20041 ttggccttaa ctatcacatt gcggtggtgc tggtcggcgt gctgatgatg atgtacgtcc
    20101 tgttcggcgg catgctggcg accacctggg tacagattat caaagccgtt ctgttgctgt
    20161 tcggtgccag ctttatggcc tttatggtga tgaaacacgt cggctttagc ttcaacaatc
    20221 tgttcagcga agcgatggcg gtacacccga aaggtgtcga tatcatgaag ccaggcgggc
    20281 tggtgaaaga tccgatctca gcactgtcgc tggggctcgg actgatgttt ggtacggcgg
    20341 gcttgccgca tattctgatg cgcttcttta cggtcagcga tgcccgcgaa gcacgtaaga
    20401 gcgtgttcta cgccaccgga tttatgggct acttctacat cctgaccttt attatcggct
    20461 tcggcgcaat catgctggtt ggtgcgaatc cggaatataa agatgctgcg ggccatctga
    20521 ttggtggtaa caacatggcg gcagttcacc tggccaatgc ggtgggcggc aacctgttcc
    20581 tcggctttat ttcagcggtt gctttcgcca ctatcctcgc ggtggtagcg gggctgacgc
    20641 tggcgggcgc atcggcggtg tctcacgacc tgtacgctaa cgtcttcaaa aaaggcgcga
    20701 ccgaacgtga agagctgcgg gtatcgaaaa tcaccgttct gattttgggt gtgattgcca
    20761 ttatcctcgg cgtgctgttt gagaatcaga acatcgcctt tatggtgggg ctggcgtttg
    20821 ccatcgcggc gagctgtaac ttcccgatca ttctgctttc catgtactgg tcgaaactga
    20881 ctacccgtgg cgcaatgctg ggcggctggt taggactgat tactgcggtg gtgctgatga
    20941 tcctcggtcc gacgatttgg gtacagatcc tcggtcacga aaaagccatc ttcccgtatg
    21001 aatacccggc actgttctct atcagcgtgg cattcctcgg tatctggttc ttctcggcaa
    21061 cggataattc agcggaaggt gcgcgcgagc gtgaactgtt ccgtgcgcag tttatccgct
    21121 cccagactgg ctttggcgtt gagcaaggcc gcgcgcatta atctccacta gcctctggtc
    21181 acctgaccaa cggtaaaaag gtgcgatgta ctgcaccttt ttaccgatta caccgccaaa
    21241 gcgttattgg ttctcttcaa tcagcaacgc ttcaagcaaa tcgagatcgt gcagtaattt
    21301 ttgcagcgtt tcgttactga tctcccgcgt ggcgcgcagg tggtacagtt cagcacgttc
    21361 agaacgcaat gccgccagac ggaagcgacg ctcaaggttc tcttcctgca cggaactttc
    21421 aacgtcgtta cgtccatcgg cgcgacgacg caggttacca atgacgcgag aactaacctc
    21481 tgtgagcagc tggttatcga tattctcttc ggtatccgcc gccagacgct cctccatttt
    21541 ctggatcgcc acaatcgcca cttctgccgt tgccgctcgc gcaatacgtt cctctttcaa
    21601 ttgttgcgaa tggtccgcga cttcaatgtg ttgtagcaga ataggcaaca tcaccacgcc
    21661 gacaaacagc gagaagagaa tgacaccagc cgccaggaac accagctcat agcgcgccgg
    21721 gaagacgtta ccatccggca agagcagcgg gattgagagc acaccagcca gagtgatcgc
    21781 cccacgcacc ccggcgaaag acgcgatcag gatttctcgt gtggtccacg aaccaaactc
    21841 catcggcttc ttcttcagga agcggttgct gaactttttc atcgtccaca gccagccgaa
    21901 acggaccagc atcagcgccg cataaatcag aatgatatcg gtaaacagca tccagatttc
    21961 gacgttaggg tcgatttctg ccgccatcag cgacgtctcc agaatacccg gcagctgcag
    22021 acctaacagc aggaatacca tgccgttaaa gacaaattcc agcatcgccc aggtactgtt
    22081 tgcgcgcagg cgcattgcca gcggcgcacg gcgcatcaca ccggaacggg tgatggtcat
    22141 cccggcggca accgcagcga ggataccgga aacgccaata tgttcggcaa tcagataaga
    22201 agcgaatggc agcaggaaca gcaggacgat ctgcgtcgcg ggttcatcac cgccccagcg
    22261 gctgaggaat cgcagcgagc gaccgtacag ccagctcacc acaaaaccgg cgagaatacc
    22321 gccaatggcc actttcataa attcgaccgt cgcaccaccg acggtgaaga tcatcgtccc
    22381 catcgccact gccacggcaa acttcaacga caccagaccg gaggcgtcat tcatcaacgc
    22441 ttcgccctgc aaaatgccca tgattttttt cgggatgcgc ccttcgccaa caatccccga
    22501 gagcgccaca gcatcggtcg gagaaagcac cgccgccagc gcaaaggcgg ggatcagcgg
    22561 aatgcccggc accacccagt aaataagaaa gccaatgccg accacggtga ccaccaccag
    22621 cgccagcgcg aggccgaaaa tctctcgacc atgttcgaga aattcacggg tcggcgtttt
    22681 ccagccatca gcgaacagca acggcgggat aaacaagact aaaaagagtt caggatcaaa
    22741 ctccacatgc aaaccaaacg tcggccacgc cagtagcgca ccgatggcga tttgcataag
    22801 cggaagcggg atctgaaagg gcatgacacg agtgaccacc ccggacagcg agaccacgag
    22861 ggtcattatc agtatggtga agaagatttc catgcgttcc ctgtttgcga tgttttttat
    22921 gatctgagaa aggcgttatg cctgaattct cattttttca gaatatcgca aaagctcaca
    22981 caatgtgcgg agaaaataaa aacgggcagc cattgccacc cgttttcaga atcatatgaa
    23041 agattagata gcccagccac ccgcatagaa ggtcaccagc gccacagcga taacgaccgt
    23101 accgatgttc aacttgcgcc attcgccgga aaccagacga ccaatcacca gagtcgcgaa
    23161 gccgatcatg atgcctgtta cgatgttaca ggtcagcacg atgaataccg ccgtaaccag
    23221 acccgccatc gcatcaacaa aatcagcaaa gtcgattttc gccacgttac tcagcatcag
    23281 caggccaacg tacatcagcg ccggagccgt tgcatacccc ggaacgaggt aagagagcgg
    23341 agagaggaac agaatcagca ggaacagcac gccaacggtg atagccgtca aaccggtttt
    23401 accgcccgcc gccgtacccg ccgcagactc gatgtatacc gctgccggag ctgcacccac
    23461 caggccagag aaaacgctgc tcatggagtc agtggtcagt gctttcccgc cgtcgatgat
    23521 ctgcccatct ttatccagca ggttcgcctg gccggcgacg gcacggatag ttccggtggc
    23581 atcaaatact gccgtcatca ccagcgccag aacgcttggc aggactacag gattcagcgc
    23641 gcccataatg tccaggctgc caattagtga attgccgttt tcatcgctca atgaaggcat
    23701 ggcgaaaacg ccggagaaat ggacgttagg atcgaagatc aaaccgacaa ttgagatacc
    23761 gataatggtc agcagaatgc caccagggac tttcagtttt tccaggccga taatcactgc
    23821 cagccccacc aacgacatca tcaccggaaa ggtcgtgaaa tcccccagcg cgacgggcag
    23881 accatcaagt gggtttttaa tcaccagacc gacaccatta gcagcaatca gcagcaggaa
    23941 cagaccgata ccaatccccg tgccgtgcgc cacaccgtgc ggcaagttgc gcaaaatcca
    24001 gctacggata cccgtggcag aaatgaccgt aaacagcaca cccatcagga acacggcacc
    24061 cagcgcgaca ggtacgctaa tatgttgccc cagcaccaga ctgaatgcgg taaacgccgt
    24121 cagggagatg gcgcaaccaa tcgccaacgg cagattagcc cacagaccca tcacgataga
    24181 accgagtccg gcaaccagac aggtcgcaac gaaaactgcc gcaggcggga agcctgcttt
    24241 acccaacatg cctggaacga cgataaccga gtagaccatc gccagaaatg ttgttaaccc
    24301 ggcaaccact tcctgacgga cagtgcttcc acgttgtgaa attttaaacc aggcgtcgag
    24361 tgaaccgccg gtacgcgctg atggcgtaga cataagaaaa catcccctga gagtttaatt
    24421 ttcgtcaaga tgcgtggcgg acaatcccct gccagcgaaa cgttatcttc ctgctccagg
    24481 tttgcgacaa attggtgcgt cgcaaaaaag caaacgttta gctccgcgca tacaaaacgg
    24541 gtggtaaaaa aagcaaacga ttatccggcg atcatagcgg ctttttcaac cgaagtttgc
    24601 tgcttttttc tcttttattg tcttgacgat gaaaaaccag gcaattgatg cgcaaacgtt
    24661 atcgtcgatg cgcaagattg cagcgttatc gagccgtata ccgttcagga ttgcgataaa
    24721 aaatcgtctg agatgacaaa aatattgcgg ctaaaaagtg tacttttaca ctggcgaaga
    24781 aacgccggtg acatacaatt aaagcatcaa caccaaccgg aacctccacc acgtgctcga
    24841 atgaggtgtg ttgacgtcgg gggaaaccct cctgtgtacc agcgggatag agagaaagac
    24901 aaagaccgga aaacaaacta aagcgccctt gtggcgcttt agttttgttc atcttccagc
    24961 aagcgtgcgc ctgtgccttc ttctcctaag cggtcgcccg ggttacgcaa tgggcaatca
    25021 ctgcgcgaaa ggcagccaca accaatacat ccgtccagct cgtcacgcag cgccactaag
    25081 gtatgaatgc gccgatccaa ctcttctcgc cattgggacg aaagctgttt ccactctttc
    25141 gcacttaatg tatgcccttc gggcaacacg ccaaatgctt cacgaatggt cgccagcgga
    25201 atgccaatac gctgagcaat tttgatgatt gcgacatatc gcaacacatc acgtttatat
    25261 cgccgctgat tgccactatt acggatactg ctaatcaacc ctttgctttc atagaaatgc
    25321 agcgccgata ccgccacacc gctgcgtttt gccacttcgc cgggggttag cagcgcttta
    25381 atgcggggta atttcttttc cataaatcgc tttacctcaa gttaacttga ggaattatac
    25441 tccccaacag atgaattaac gaactgaaca ctgaaaagag gcagatttat gtcccatcag
    25501 aaaattattc aggatcttat cgcatggatt gacgagcata ttgaccagcc gcttaacatt
    25561 gatgtcgtcg caaaaaaatc aggctattca aagtggtact tacagcggat gttccgtact
    25621 gtgacgcatc agacactagg tgattacatt cgccaacgcc gcctgttact ggccgccgtt
    25681 gagttgcgca ccaccgagcg tccgattttt gatatcgcaa tggacctggg ttatgtctct
    25741 cagcagacct tctcccgcgt tttccgtcgg cagtttgatc gcactcccag cgattatcgc
    25801 caccgcctgt agttttattg cccgcgcgtt aactcccgcg cgggtaattg ctccatccat
    25861 tgcataaaca cctgcggcgg catcgccttc gcaaagaacc atccctgaca atagcgcacg
    25921 ccgcgtttgc gcagccagtt aacctgctcc tcagtttcga cgccttcagc gatcgttttt
    25981 aaccccagac tgtgcgccag ctcgatgata tgttccgcaa tcaaatgact ggttttatgg
    26041 gtggtcagcg tctcaacaaa cgatttgtcg attttcaaaa tatcgacatt caatgattta
    26101 aggttatgca agttagagta gccaatacca aaatcatcaa ttgccacttc gtaaccagcc
    26161 tggcggaaag ccagaataat cggcgtcatt ttgtcgacat caagaaatgc atgttcagtc
    26221 acttcaaatt taatttgctg cggacgcacc gcatattgct ctgttttctg gttggttcgc
    26281 gctatcaacc gtgacgtatg aaaatcggag gccgacaggt taatagaaac atagcgatcg
    26341 acatgtgttg ccaggtacac gcccagatcg cggaagacat tatcaataac ataatcagtt
    26401 atctgttcga tcatcccctc cttttctgcc agcggaataa actctgccgg attcattact
    26461 tgcccctgct caccaggcca acgtaacaat gcttcagctc cgatacattt ttctgttttg
    26521 atatcgatta ttggctggta ataaaggcaa agttgatgtt tttcgagggc gcgttgcaat
    26581 ttgcgtttgg gagacaaata gttttgtcga atacgtagcc agagtaatag cagaacaaga
    26641 ctccccaaaa taccggcggg caacgcaaaa ataagatgat tataaaaatg agttataaga
    26701 cgttgatatg aagtcgcaac aatggctgca attgggcgtt ttgtcgaata aactgtcgca
    26761 tataaatagc catttcgttg tacggttaaa tcattcaaat gaatcagcgg agaaaacgtt
    26821 gctgccgagg cctcgttgct taacgagaaa aaggttttcg tcaccgtatc atacactccc
    26881 cattgcaatg tcggatcatc agacatcact tcactccaga agagagggtt gataaccgcc
    26941 acataatttc cccgctgcat gtaggtcatt ttatagccag agaaaaaagg cgtatcacgg
    27001 taataataga tagaaacgtt aggttcacgc ttataatcgg ccggtgcaat cgtatagcca
    27061 tttacagacg ctatcagcga tgagcataaa aaatggttat cacgggcata gatcaattca
    27121 ttaatataaa gatagccacg aataatattc aacattcgct tttgatgggc tggagtgcaa
    27181 acttgcccct gatagcgttc tgctgcatcg ctcactaaat ctgcctggcg aatgaccagc
    27241 tcggatttat ccagcgccag ctgagcaaaa gtttggagct gactattcac ttctgatttt
    27301 gcccaaagga aggcaatcca tagcgacaga atgatgggaa agagaactaa aaagataatg
    27361 cccggcaacg ccagtaattg gtgtcgtgca cgatgactca ttgctatcaa tttcctgcct
    27421 gtttataccc ggccattgca aggtgaaaac cctgggcacc gacgtcttta gataaatctt
    27481 acggtataaa tagcactttt gcctgatttg aaattgatac ctttttaatg gtcacctgga
    27541 taaaaataat tcattgatat tcaaaactaa ttttctacag gaatgcagag gcggcgggaa
    27601 gatatgccct taatgccact gaaaaaccac ataatctgta cagcaataaa agtcacggcc
    27661 taatctggct aacaaacaat caattgtgat atagttcaca aaattaatga aacaaacaga
    27721 gtgtttcatt tttgtgtcct gcaagccccg cgatagccag agtgtaccac gcctcccgtg
    27781 aacaacgccg cgctgtccag ggcatcggct cttttcagag gaattgtttg atggctaccc
    27841 tcaccactgg cgtggttcta cttcgctggc aacttcttag tgccgtaatg atgtttctgg
    27901 ccagcacgct caacatccgt tttcgtcggt ctgattatgt cgggcttgca gtgatcagca
    27961 gcggtctggg cgtggtttct gcctgctggt tcgcaatggg gttgcttggc atcacaatgg
    28021 cggatatcac cgccatctgg cacaacatcg agtcggtgat gattgaagag atgaatcaga
    28081 caccgccaca atggccaatg attttaactt gatatgtaga agcctccata atggaggctt
    28141 ctttttttat ggctggcaga tgctttaatc atccacctta aaacaatata acctattgtt
    28201 ttaatgacaa atcagaacgg aatatcgtca tcaaaatcca tcggcggctc gttagacggt
    28261 gctgccggag cggactgctg cgggcgagac tgcgcgccgc cgctgaactg attgccgccc
    28321 tgcggctgct gaggctgacc ccaaccgccc tgcggctgac caccaccgat attgccacct
    28381 gcaggagcgc caccaccctg acgaccaccc agcatctgca tggtgccgcc aacgttcacc
    28441 acgacttctg tggtgtagcg atcctgaccg gattgatcgg tccatttgcg ggtacgcagc
    28501 tgaccttcga tataaacctg agaaccttta cgcagatatt cgctggctac ttctgccagt
    28561 ttgccgaaca gcacaacgcg gtgccactca gtctgctctt tcatctcgcc ggtcgctttg
    28621 tcacgccagg attcggaagt agccagcgta atgttggcaa cagcgccacc atttggcatg
    28681 tagcgtactt ccgggtcctg acccagatta ccaacgagaa taaccttgtt tacgcctctg
    28741 ctggccatgt tcgtgtctcc tgaaaaaaaa tcgttctgaa taagtgtaaa cgcgcgattg
    28801 taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa caaacctggc
    28861 cagacattgt tacacaacac tccgggtaat gcattccaat actgtatatt cattcaggtc
    28921 aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc gggcaaaaaa
    28981 gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc gcacccataa
    29041 tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga ccgggctttc
    29101 gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc agcgccgtta
    29161 cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaga agccggacgt
    29221 cgaccatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga cgtctcataa
    29281 cccgcgctct acggtgggga caatcaccga aatccacgac tatttgcgtc tgctgtacgc
    29341 tcgcgttggt gagccgcgct gcccggacca cgatgttccg ctggcggcgc aaaccgtcag
    29401 ccagatggtg gataacgtgc tttcgcagcc ggaaggcaag cgtctgatgc tgctcgcgcc
    29461 aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gaaaatctgg caagccaggg
    29521 ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc cgaaactgga
    29581 actgcaaaag aaacatacca ttgaagtggt ggttgatcgc ttcaaggtgc gtgacgatct
    29641 cacccaacgt cttgccgagt cgtttgaaac cgcgctggag ctttccggtg gtaccgcggt
    29701 agttgccgat atggacgatc caaaagcgga agagctgcta ttttccgcca acttcgcctg
    29761 cccaatttgc ggctacagta tgcgtgaact ggaaccacgc ctgttttcgt ttaacaaccc
    29821 ggcgggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg atcctgaccg
    29881 cgtgatccag aatccggaac tgtcgctggc aggtggtgcg atccgaggct gggatcgccg
    29941 caacttctat tacttccaga tgctgaaatc gctggcagat cactataagt tcgacgtcga
    30001 agcgccgtgg ggcagcctga gcgcgaatgt gcataaagtg gtgttgtacg gttctggcaa
    30061 agaaaatatt gaattcaaat acatgaacga tcgtggcgat acctccattc gtcgtcatcc
    30121 gttcgaaggc gtgctgcaca atatggagcg ccgctataaa gagacggaat ccagtgcggt
    30181 acgtgaagaa ttagccaagt ttatcagcaa tcgtccgtgc gccagctgcg aagggacgcg
    30241 tctgcgtcgg gaagcgcgcc acgtttatgt cgagaatacg ccgctacctg ctatctccga
    30301 catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag gtcagcgggc
    30361 gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc tcgttaacgt
    30421 cggcctgaat tacctgacac tttcccgctc ggcagagaca ctttctggcg gtgaagccca
    30481 gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt acgtgctgga
    30541 cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgctgggta cgcttatcca
    30601 tctgcgcgat ctcggtaata ccgtgattgt ggtggagcac gacgaagacg cgattcgcgc
    30661 cgctgatcat gtgatcgata tcggtccggg tgcgggtgta cacggcggtg aagtcgtcgc
    30721 ggaaggtccg ctggaagcaa taatggcggt gccagagtcg ttgaccgggc agtacatgag
    30781 cggtaaacgc aagattgaag tgccgaagaa gcgcgttccg gcaaatccgg aaaaagtgct
    30841 gaaactgaca ggcgcacgcg gcaacaacct gaaagacgtg acgctgacgc tgccggtggg
    30901 tctgtttact tgcatcacag gggtttcagg ttccggtaaa tcgacactga ttaacgacac
    30961 actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac cggcaccgta
    31021 tcgcgatatt cagggactgg aacatttcga caaagtgatc gatatcgacc aaagcccaat
    31081 tggtcgtacc ccacgttcca atccggcaac ctataccggc gtgtttacac ctgtacgcga
    31141 actgttcgca ggcgtaccag aatcccgtgc gcgtggttat acgccagggc gtttcagctt
    31201 taacgtccgt ggcggacgct gcgaggcctg tcagggcgac ggcgtgatca aagtggagat
    31261 gcacttcctg ccggacattt acgtaccgtg cgaccagtgc aaaggcaaac gctataaccg
    31321 tgaaacgctg gagattaagt acaaaggcaa aaccatccac gaagtgctgg atatgaccat
    31381 cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcacgtaagc tgcaaacgct
    31441 gatggatgtt ggcctgacgt acattcgcct cgggcagtcc gcaaccacac tttctggtgg
    31501 tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg ggcagacgct
    31561 gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc aactgctcga
    31621 cgtactgcat aaactgcgcg atcagggcaa taccattgtg gtaattgagc acaatctcga
    31681 cgtgatcaaa accgctgact ggattgtcga cctgggaccg gaaggcggca gtggcggcgg
    31741 cgaaatcctc gtctccggta cgccagaaac cgtcgcagag tgcgaagctt cgcatacggc
    31801 gcgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tcagagcggc cttcttcttt
    31861 cagagttgca ccagcaattt acgtttttct tccggcagca aattcaccgc ctgctgataa
    31921 gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag atacacggtg
    31981 ctccagtgcg ctttattcag atggcggctt ggacgcacat cgctgtgctg ctgacgtagc
    32041 agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc tacttctttc
    32101 accatcgcaa acagcacatc ttccactttg atctgcgtcg ctttccagtc gttatgcacg
    32161 ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat ggtcattttt
    32221 cactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg atgttccccc
    32281 agccagatgc cttgccaggt gccggtctga atacgccctt tatgcaccgg caatacaagc
    32341 gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc ctcatagtca
    32401 tgctcataat ttccgttatc gggaacggcg cggaggaaaa aacgctccat gtcgtggcgt
    32461 acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg cagcaacaga
    32521 tgcagtaaac cgatgttaac gcgcggcata tcagccagct gattcagaat ttcatccgtt
    32581 accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata ccacatgtgc
    32641 tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg ataaaggtgt
    32701 gattaaaaaa acagcattcg ggagagtatt acgctctccc gctctgtcag tattctgaat
    32761 tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag gtagagttgg
    32821 aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta atatcgttat
    32881 cggaatcacc ataaaaaatt cggatatttt tatcctgcag ccattgcgat tttgtatttt
    32941 gccctggttt atcgcccgca aagatcaccg gattcatgtt ggtggcagga atatgaaaat
    33001 tatccgccag cgtttttgaa accgtttctg ttttggtcgg gctacgacca gtcacaaaga
    33061 agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc tcttttggaa
    33121 tgctgaattc atcccagcca ttgttcattt tttcccagaa cacaggattt ttcagataat
    33181 cttcgctttc tggcgagaag gtttttttgc cgcgccagaa gcccggactg gaaaaaagta
    33241 ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg ctattttcaa
    33301 tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca acgttagtac
    33361 cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt agagcgaaca
    33421 ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct aaatagtcag
    33481 tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat aacgccagca
    33541 gc
//
LOCUS       DAZUCC010000027        33366 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZUCC010000027 DAZUCC010000000
VERSION     DAZUCC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.027"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            146..688
                     /gene="zapC"
                     /locus_tag="ACHY09_001149"
     CDS             146..688
                     /gene="zapC"
                     /locus_tag="ACHY09_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFS3884151.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFNLEQAV"
     gene            complement(685..1794)
                     /gene="ycbX"
                     /locus_tag="ACHY09_001150"
     CDS             complement(685..1794)
                     /gene="ycbX"
                     /locus_tag="ACHY09_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFS3884152.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEVLATAPAKIYGAAA
                     ADDTVNITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            2038..4146
                     /gene="rlmKL"
                     /locus_tag="ACHY09_001151"
     CDS             2038..4146
                     /gene="rlmKL"
                     /locus_tag="ACHY09_001151"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFS3884153.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPVMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4158..6065
                     /gene="uup"
                     /locus_tag="ACHY09_001152"
     CDS             4158..6065
                     /gene="uup"
                     /locus_tag="ACHY09_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3884154.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6195..7448
                     /gene="pqiA"
                     /locus_tag="ACHY09_001153"
     CDS             6195..7448
                     /gene="pqiA"
                     /locus_tag="ACHY09_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFS3884155.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADDPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7453..9093
                     /gene="pqiB"
                     /locus_tag="ACHY09_001154"
     CDS             7453..9093
                     /gene="pqiB"
                     /locus_tag="ACHY09_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFS3884156.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9090..9653
                     /gene="pqiC"
                     /locus_tag="ACHY09_001155"
     CDS             9090..9653
                     /gene="pqiC"
                     /locus_tag="ACHY09_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFS3884157.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            9909..10076
                     /gene="rmf"
                     /locus_tag="ACHY09_001156"
     CDS             9909..10076
                     /gene="rmf"
                     /locus_tag="ACHY09_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFS3884158.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10146..10664)
                     /gene="fabA"
                     /locus_tag="ACHY09_001157"
     CDS             complement(10146..10664)
                     /gene="fabA"
                     /locus_tag="ACHY09_001157"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFS3884159.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10733..12493)
                     /gene="ycbZ"
                     /locus_tag="ACHY09_001158"
     CDS             complement(10733..12493)
                     /gene="ycbZ"
                     /locus_tag="ACHY09_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3884160.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANIR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12679..13131
                     /gene="matP"
                     /locus_tag="ACHY09_001159"
     CDS             12679..13131
                     /gene="matP"
                     /locus_tag="ACHY09_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFS3884161.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13207..14259)
                     /gene="ompA"
                     /locus_tag="ACHY09_001160"
     CDS             complement(13207..14259)
                     /gene="ompA"
                     /locus_tag="ACHY09_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFS3884162.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVPGGASTKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(14615..15124)
                     /gene="sulA"
                     /locus_tag="ACHY09_001161"
     CDS             complement(14615..15124)
                     /gene="sulA"
                     /locus_tag="ACHY09_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFS3884163.1"
                     /translation="MYTSGYAHRASSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15342..15971
                     /gene="sxy"
                     /locus_tag="ACHY09_001162"
     CDS             15342..15971
                     /gene="sxy"
                     /locus_tag="ACHY09_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFS3884164.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15934..18096)
                     /gene="yccS"
                     /locus_tag="ACHY09_001163"
     CDS             complement(15934..18096)
                     /gene="yccS"
                     /locus_tag="ACHY09_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFS3884165.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18106..18552)
                     /gene="yccF"
                     /locus_tag="ACHY09_001164"
     CDS             complement(18106..18552)
                     /gene="yccF"
                     /locus_tag="ACHY09_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS3884166.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18675..20729
                     /gene="helD"
                     /locus_tag="ACHY09_001165"
     CDS             18675..20729
                     /gene="helD"
                     /locus_tag="ACHY09_001165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFS3884167.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRFYHHLDAHWQRWSSEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEVIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPSVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20761..21219)
                     /gene="mgsA"
                     /locus_tag="ACHY09_001166"
     CDS             complement(20761..21219)
                     /gene="mgsA"
                     /locus_tag="ACHY09_001166"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS3884168.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21315..21977)
                     /gene="yccT"
                     /locus_tag="ACHY09_001167"
     CDS             complement(21315..21977)
                     /gene="yccT"
                     /locus_tag="ACHY09_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFS3884169.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22150..22563
                     /gene="yccU"
                     /locus_tag="ACHY09_001168"
     CDS             22150..22563
                     /gene="yccU"
                     /locus_tag="ACHY09_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS3884170.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22608..22925)
                     /gene="hspQ"
                     /locus_tag="ACHY09_001169"
     CDS             complement(22608..22925)
                     /gene="hspQ"
                     /locus_tag="ACHY09_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFS3884171.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22983..24173)
                     /gene="rlmI"
                     /locus_tag="ACHY09_001170"
     CDS             complement(22983..24173)
                     /gene="rlmI"
                     /locus_tag="ACHY09_001170"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFS3884172.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVNIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24268..24546
                     /gene="yccX"
                     /locus_tag="ACHY09_001171"
     CDS             24268..24546
                     /gene="yccX"
                     /locus_tag="ACHY09_001171"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS3884173.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLAGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            complement(24543..24872)
                     /gene="tusE"
                     /locus_tag="ACHY09_001172"
     CDS             complement(24543..24872)
                     /gene="tusE"
                     /locus_tag="ACHY09_001172"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFS3884174.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24963..25622)
                     /gene="yccA"
                     /locus_tag="ACHY09_001173"
     CDS             complement(24963..25622)
                     /gene="yccA"
                     /locus_tag="ACHY09_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFS3884175.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(26032..27051)
                     /locus_tag="ACHY09_001174"
     CDS             complement(26032..27051)
                     /locus_tag="ACHY09_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3884176.1"
                     /translation="MGRPRKNKKDNALPPRVRSNGYSYVWKPEGSTKTIGLGRVRETS
                     IAKVWQNYELEKAKRHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQRALLAVFGK
                     VLADNVKIEQVRIFMDKRGIESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFT
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSGTCEYVINTTKGGKVIAKTLNNWWNQAKHAA
                     EKKSGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(27020..27271)
                     /locus_tag="ACHY09_001175"
     CDS             complement(27020..27271)
                     /locus_tag="ACHY09_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFS3884177.1"
                     /translation="MYELTLSPAEIQEITRYERYTKQQQQLRLHGIPFVPGPKNEPIV
                     LRSDVPRGLTAIPKVSELVSADPDFEALNNGKTKKKQKR"
     gene            complement(27338..29131)
                     /locus_tag="ACHY09_001176"
     CDS             complement(27338..29131)
                     /locus_tag="ACHY09_001176"
                     /inference="COORDINATES: protein motif:HMM:NF027788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFS3884178.1"
                     /translation="MTAFFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGG
                     NKTDRNPDLVHTLDTLDVEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSA
                     ALRATPGILDYSRAAIFALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAA
                     RHTPEKDILEEINGGLAAECKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQD
                     PQPLDDKSQVKVTAEEVNKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGI
                     ETCDSVNQNQQETEQNGQKAEQNSPNALQNEPETKQPEPVVQQEEEKVCTACNQTGGG
                     NCPDCGAVMGDATYQETFNEESQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETG
                     ETANPLIKVNGHREITSTSRLWHHLMIDLETMGKNPDAPIASIGAVFFDPQTGEQGPE
                     FSKIIDMGTCGGTVDISTIEWWLQRSGEARAAILADRIPLDDALLQLREFIDENSGEF
                     FVQVWGNGANFDNVILRRSYERQEIPCPWRYTNDRDVRTIVALGLVMDFDARSVITFE
                     GERHNALHDARYQAKYVSAIWQKLFPNQADF"
     gene            complement(29270..29776)
                     /locus_tag="ACHY09_001177"
                     /pseudo
     CDS             complement(29270..29776)
                     /locus_tag="ACHY09_001177"
                     /inference="COORDINATES: protein motif:HMM:NF018353.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
     gene            complement(29869..30057)
                     /locus_tag="ACHY09_001178"
     CDS             complement(29869..30057)
                     /locus_tag="ACHY09_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFS3884179.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(30054..30242)
                     /gene="dicB"
                     /locus_tag="ACHY09_001179"
     CDS             complement(30054..30242)
                     /gene="dicB"
                     /locus_tag="ACHY09_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFS3884180.1"
                     /translation="METLLPNVNTSEGCFDIGILLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(30253..31104)
                     /locus_tag="ACHY09_001180"
     CDS             complement(30253..31104)
                     /locus_tag="ACHY09_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS3884181.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGIATPMFIMAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPQGWPGSMMTGCSNPVRLTTHEIATSGGELMKFIMEAAIMATI
                     PALSHPEITIANGRAVTSSLAVAEYFRKPHKDVLAKISRLDCSAEFTERNFSPSEYTD
                     STGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKMI
                     HGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTVE
                     QLLKIHC"
     gene            complement(31623..31775)
                     /locus_tag="ACHY09_001181"
     CDS             complement(31623..31775)
                     /locus_tag="ACHY09_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFS3884182.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            complement(32053..32340)
                     /locus_tag="ACHY09_001182"
     CDS             complement(32053..32340)
                     /locus_tag="ACHY09_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3884183.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWKRLRTCVQPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTISGIEIVNVVHARRQFPVTP"
     gene            complement(32341..32532)
                     /locus_tag="ACHY09_001183"
     CDS             complement(32341..32532)
                     /locus_tag="ACHY09_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFS3884184.1"
                     /translation="MNTALSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VERRMAERFAKMRKERSEQ"
     gene            complement(32501..32650)
                     /locus_tag="ACHY09_001184"
     CDS             complement(32501..32650)
                     /locus_tag="ACHY09_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884185.1"
                     /translation="MLKDMKEKKEAMDRIVARWLAAQQKVNQAEYQHLQNEVHHEHSP
                     FTDGF"
     gene            complement(32719..32925)
                     /locus_tag="ACHY09_001185"
     CDS             complement(32719..32925)
                     /locus_tag="ACHY09_001185"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3884186.1"
                     /translation="MLLLAKGWSQAELARRIGIAQQSVQRWVCGISSPTAANLDKLSE
                     VTGLPQYWFFLPLNEEKKTGHRIY"
     gene            complement(32982..33353)
                     /locus_tag="ACHY09_001186"
     CDS             complement(32982..33353)
                     /locus_tag="ACHY09_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884187.1"
                     /translation="METKEIRRLRLKEWFKDRTLPPKEKSYLSQLMSGKATFGEKAAR
                     RIEQTYGMPGGYLDIEPEDITESPSSRSIALTPNQLELLQIFSAFPDNEQQEIIKELK
                     NKKEAMEDLVARWLARQGRRA"
BASE COUNT         8358 a         8081 c         8496 g         8431 t
ORIGIN      
        1 taatcagggc tttattttaa gccctggttg ttttatattc atcgctgttg cttatttaga
       61 cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg tgttgctgcg
      121 gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact ggcgttggta
      181 ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc tatttcgctc
      241 acgttttgcg cgcaaaatgt tgactccaga tgccttttct cccgcaggct tttgcgttga
      301 cgacgccgcg ctctatttct catttgaaga aaaatgccgc gattttaatt tatctaaaga
      361 acaaaaagcc gaactggtgc ttaatgcgct ggtagcaatt cgttatctca aaccgcaaat
      421 gccgaaaagc tggcattttg tttcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc
      481 ggcctgcgtt tggttgagcg acactcacga gcaagtcaat ttgctggtcg ttgaatctgg
      541 cgaaaatgcc gcactatgcc tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat
      601 gcagctgggc gacgccatca aaatcatgaa cgacaggctg aaaccgcagg ttaatgttga
      661 cagcttcaac ctcgaacagg cagtctaacg cgtcagctta agcgcagtct tcggtacaca
      721 gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg gcgtgacttc
      781 gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag aataagggat
      841 acgaattccc tgattttcta attgctccag caacacctgc tggttatttc cacgaaatgc
      901 ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt tgacggtatc
      961 atcagcggcg gctgcgccgt aaattttagc cggagccgtt gccagaactt ccacctcatc
     1021 gccaacgcga attacgccgc tattacgggc aattaaattc tgaccaaaat cgacatcgcc
     1081 gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg ccggatgttt
     1141 ttgccctttt tctggactga cggtggtgaa aatacagcgg ctacaaggtt taaccacatc
     1201 aaacaccaca tcaccaatgc gaatcacttt ccagctatct tcttcccagg ctgacgcgcc
     1261 ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac aacgttgttg
     1321 gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag caaatgacag
     1381 gggtacagtg ttgtggcgtt tcacgcgccg cgtcatttgt ggccccaccc agcgcaactg
     1441 cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggtg caattcgcgc
     1501 ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg
     1561 aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca caggtgaagg
     1621 agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt caggttccgt
     1681 gatcataaag atgcgatcga aggccagacc actgacatct gccagggcat gtgtaagacc
     1741 aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg ccacggtgcg
     1801 gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta gctataatgc
     1861 gcaacaattt tcttagcgcc tgtcccatta ggctatttta tttgtcagtt tggcccaggg
     1921 aagtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct gtccatgtcc
     1981 agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag tgacgatatg
     2041 aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa
     2101 aacctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa gggcgacaca
     2161 cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat gttgccgctg
     2221 ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc gatcaactgg
     2281 acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt gaatgacacc
     2341 atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcacg
     2401 cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc
     2461 tggctgcata aagaaaccgc cagtattgcc cttgatctca gtggtgatgg tttacatctg
     2521 cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc agccgcgatt
     2581 gttatgcgat ccggctggca gccaggaaca ccgctgctcg atccgatgtg tggttccggt
     2641 acgttgttga ttgaagcggc gatgctggcg accgatcgcg caccaggctt gcaccgtgga
     2701 cgttggggct ttagcggctg ggcacagcat gatgaagcca tctggcagga agtgaaagca
     2761 gaagcgcaaa cccgcgcccg taaaggcctg gctgagtata gctctcattt ttacggttcg
     2821 gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt
     2881 gaactgatca cctttgaagt gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg
     2941 ccgtacggta cagtgttgag caacccgcca tacggtgaac gtctggacag cgaaccggcg
     3001 ctgattgcgc tgcatagtct gctgggccga atcatgaaaa accagttcgg cggctggaat
     3061 ctctctttat tcagtgcctc gccggatctg ttaagttgcc tgcagttgcg tgcagacaaa
     3121 cagtacaaag cgaaaaacgg tccattagac tgcgtgcaga aaaattacca cgttgccgaa
     3181 agcacaccag acagcaaacc ggtaatggca gcggaagact acgccaaccg tctgcgtaag
     3241 aacctcaaaa aattcgagaa gtgggcgcgt caggaaggca ttgaatgcta ccgcctgtac
     3301 gacgccgatc taccggaata caacgttgca gttgaccgct acgccgattg ggtggtggtg
     3361 caggagtatg cgccgccaaa aactattgat gcgcacaaag cgcgtcagcg tctgttcgat
     3421 attatcgctg caaccatttc ggtattgggg attgcgccaa acaaactggt gctgaaaacc
     3481 cgtgaacgcc agaagggcaa aaatcagtac cagaaactgg gcgagaaggg cgagtttctc
     3541 gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattacct cgataccggt
     3601 ctgttcctcg atcaccgtat cgcccgtcgt atgctcggtc agatgagcaa aggcaaagat
     3661 ttcctcaacc tgttctctta taccggcagc gccaccgtgc acgcgggatt aggcggtgca
     3721 cgcagcacta ccaccgtgga tatgtcgcgt acttatctgg agtgggcaga acgcaacctg
     3781 cgtctgaatg gcctaaccgg gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg
     3841 ctgcgtgagg caaatgaaca gttcgatctg atttttatcg atccgccaac cttctctaac
     3901 tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atctggcgct gatgaaagat
     3961 ttgaaacgtc tgctgcgtgc tggtgggacg atcatgttct cgaacaacaa acgtggcttc
     4021 cgtatggatc ttgacggact ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa
     4081 acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct gattaccgca
     4141 gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg tcgttcagcg
     4201 acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt gtttgtctgg
     4261 tgggccgcaa cggcgcaggc aaatcgacgt taatgaaaat cctcaaccgt gaacaagggc
     4321 tggatgacgg tcgcattatt tacgagcaag atttgattgt agctcgtctg caacaggatc
     4381 cgccgcgtaa cgttgagggg agtgtttatg atttcgttgc cgaaggcatt gaagaacaag
     4441 cggaatacct gaaacgctat cacgatattt cgcgcctggt gatgaacgac ccgagcgaga
     4501 aaaatctcaa cgaactggcg aaggttcagg aacagttgga tcaccacaac ctgtggcagc
     4561 tggaaaaccg cattaacgaa gtgctggcgc aactggggtt agatcctaac gttgcgctgt
     4621 cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg gtgagcaatc
     4681 cgcgcgtatt gctgctcgac gaaccgacta accacctgga tatcgaaacc atcgactggc
     4741 tggaaggatt ccttaaaacc ttcaatggga cgataatttt catctcccac gaccgttcgt
     4801 ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctt gtgacctatc
     4861 ctgggaatta cgaccagtac ctgctggaaa aagaagaagc cctgcgcgtg gaagaactac
     4921 aaaatgccga gttcgatcgc aaactggcgc aggaagaggt gtggatccgc caggggatca
     4981 aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcaatg cgtcgtgagc
     5041 gtggcgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag gccagccgct
     5101 ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttgacggt aagcaactgg
     5161 tgaaagattt ttctgcccag gttctacgtg gcgacaaaat tgccctgatt ggtccgaatg
     5221 gatgcggcaa aaccacgctg ctgaaactga tgctcggtca gcttcaggcg gacagcgggc
     5281 gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgt gcggaactgg
     5341 atcccgataa aacggtgatg gataaccttg ctgaaggtaa gcaagaggtg atggttaacg
     5401 gcaagccacg tcatgttttg ggctatttgc aggacttcct gttccatccg aaacgggcga
     5461 tgacgccggt acgtgcgctt tctggcggtg agcgtaaccg cttgctgttg gcgcgtttgt
     5521 tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt gatgttgaaa
     5581 cgctggaact gctggaagaa ctgatcgaca gctaccaggg cacggtattg ctggtgagcc
     5641 acgatcgtca gtttgtcgat aacaccgtta cagaatgctg gatcttcgaa ggcggcggta
     5701 aaattggtcg ttatgtcggc ggttatcatg atgcccgtgg tcagcaagaa cagtatgtgg
     5761 cgctcaaaca gcctgcggtg aaaaaaatcg aagaagccgc cgcgccaaaa gcagaaactg
     5821 taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag cagctaccgc
     5881 aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaggtg gcggatgctt
     5941 ccttcttcag tcagccgcat gagcagacgc aaaaagtgct cgccgatatg gctgctgcag
     6001 agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta aaaaatggtg
     6061 gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa ttgcctgatg
     6121 aactgggttt atcaggcatg aaaaccaaca ctttgttagt catctgtaaa atagcgcatc
     6181 attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg tgctcgcagt
     6241 gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca tgtccccggt
     6301 gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc gcctatgcgt
     6361 tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat atgaacgttg
     6421 cgggagtcac cagtgaaatt acattactgg aaattcccgg cgtgcttttt tctgaggact
     6481 acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg ttttgtctga
     6541 taaccattct gttactggtg aatcgcgcag aattaccggt ccgtttaaaa gagcaactgg
     6601 cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc gcgggtgtgc
     6661 tggtcagttt cgttaaactg atggcttacg gcagcattgg cgtaggcagc agctttctcc
     6721 cctggtgttt attttgtgtc ctgcaactgc gtgcttttca gtgcgttgat cgtcgctggt
     6781 tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacct ggcgtcacgg
     6841 ggatacgcca ggggttacgt tcgtgctcct gttgtacagc aattcttcca gctgatgatc
     6901 cggtgtgtcc gcgctgtggt actaaagggt acgttcgtcg tagaaacagc ctgcaatgga
     6961 cactcgcgct gcttgttacg tccatcatgc tgtatcttcc ggctaatatt ttgcccatca
     7021 tggtgacgga tttattaggc tcgaagatgc catcgacgat tctcgctggg gtcatcctgt
     7081 tatggagcga aggatcttat cccgtcgctg cggttatctt tctggccagt attatggtgc
     7141 caacgttaaa gatgatcgcc atagcgtggt tgtgttggga tgccaaaggg catggcaagc
     7201 gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc cgctggtcga
     7261 tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga ggtttaatga
     7321 gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg acaatgtttt
     7381 ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca gagcatgagg
     7441 agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt gaagaactgg
     7501 tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt tcttttttat
     7561 cattacagcc atcaggggcc ggaagtgacc ctgatcaccg cgaatgcgga aggaatagaa
     7621 ggtggtaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga aagcgccaca
     7681 ctggctgatg atttgacgca cgttgaaatc aaagcgcggc tgaattccgg tatggaaaaa
     7741 ttgctgcata aagataccgt cttttgggtg gtgaaaccgc agattggtcg agaagggatt
     7801 agcggcctgg gaacgctgct gtctggtgtt tatatcgaac tgcagccagg cgcgaaaggc
     7861 agcaaaatgg ataaatacga tttgctggac tcaccaccgt tggccccgcc tgatgcgaaa
     7921 ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg agatcccgtg
     7981 ctgttccgtg gctatcgggt aggttcggtt gaaaccagca cctttgatac gcaaaaacgc
     8041 aatatcagct atcaactgtt catcaatgca ccttatgacc gactggtgac cagcaatgtt
     8101 cgcttctgga aagatagtgg cattgcggtt gatttgacgt cagcgggaat gcgtgtggag
     8161 atgggttcat tgacaacgct gcttagtggc ggtgtcagct ttgatgtgcc ggaaggtctg
     8221 gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga tcagaagagc
     8281 attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa agattcggta
     8341 cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg taccgtaagc
     8401 aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta ccgtattccg
     8461 gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa tgcggatgtt
     8521 gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa aaccggaaac
     8581 ctggtcactg gcgcactgta tgttgatctc gatttctatc caaatacgcc tgcaataacc
     8641 ggtattcgtg aatttaatgg ttatcagatt atcccgaccg ttagcggcgg tctggcgcaa
     8701 atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt gaatccgatg
     8761 attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa cctgcaaacg
     8821 acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagct gccgacggat
     8881 atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc tggctccgca
     8941 gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg agaactgcaa
     9001 ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc gaaggacaaa
     9061 aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg attgcggcac
     9121 tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag ttacctgtgg
     9181 tgcagagcgg tacacaaagc accgccagcc agggtaatcg cctgttatgg gttgagcagg
     9241 tcgctgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt gatgtgaagt
     9301 atgtgattgc caacaacaac ctgtgggcca gcccgttgga tcaacagttg cgcaacactc
     9361 tggttgccaa cctgagcacg caactgcccg gctgggtggt tgcctcccag cctctgggaa
     9421 gcgcccagga tacgctcaat gttaccgtaa cggagtttaa cgggcgatat gatggcaagg
     9481 tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa cgtccgttcc
     9541 gtctggaagg ggtgcaaact caggatggtt acgatgaaat ggttaaagtg ctggccagtg
     9601 tctggagtca ggaagccgct tctattgcgc aagagataaa gcgtctaccg taatcataaa
     9661 gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg tttctaaggt
     9721 gcggaaaaag ttgttcctct tcacattttt tgtacaactg acatgcccgt gtagctcaca
     9781 aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg gtattgctta
     9841 actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca gtatgaggga
     9901 aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa cgtggttatc
     9961 aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg aatcaaaggt
    10021 cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg gcctgattct
    10081 gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac tctgtctgat
    10141 tataatcaga aggcagacgt atcctggaac agaccgactt tcaggtcgct ggcggtatag
    10201 atcagacgac catcaaccag cacttcgcca tctgccagac ccataatcag acgtcgatta
    10261 acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag tacctgaccg
    10321 gtgaatttca cttcgccaac gcccagcgcg cggcctttac cttcgccgcc cagccagccg
    10381 aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg cataaccgga
    10441 tcgccaatga agtggcatcc gaagaaccac agatccggat tgatatccag ttctgcttca
    10501 acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg gtccatcatc
    10561 agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc gcgaccagag
    10621 gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc tgtaagcctt
    10681 attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc gatcagttcg
    10741 gaataaacca gttcagccaa cgcaatggcc atggaaaacg gtgacgtcct tcctgttgcg
    10801 atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct tcgccatccc
    10861 acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc cagatggtga
    10921 atttgccttc ttctaccgct ttcaccagtt cactgtgaag acttaaatgg cgaatgttag
    10981 cagtggggat aatgacacct tgtttcccgg ttaactcacg ttgctggcaa atagcaaaga
    11041 agccttcgat tttctcattt aaaccaccga ccggctgggc acgaccgaac tgatcgactg
    11101 aacctgtgat agcgatactc tgattcaccg gcacatcggc gagggcgctt atcagggcgc
    11161 agagttcagc catcgaggca ctatcgccat ccacttcact gtatgactgc tcaaatgtca
    11221 gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg aacgcctgca
    11281 tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt tcgatgtcgg
    11341 tgaactcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg ccaaaagcgc
    11401 gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg cgttcgcctt
    11461 cggtttcaat caggatttgc tcctgaagaa tctcatcctg catgcgttca gcgaggaaac
    11521 cttcacgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag aaggtgtcgc
    11581 catcgcacag ggaggcgacc tctttacact ggcggaggat ccactgcggg ctaagcggaa
    11641 gtgtttcttg ttcaccggtg tagcgggctg cttcgcggat aagtaccggc caggcatccg
    11701 ctcccggtgc aggtaagtga ttatgtcttg cggtcaatgt tacccagcga caccactggc
    11761 ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa atagcctgct
    11821 ctgaaagctc tggttccatc tcctggaaat cagccaatga ttcgcgttcg cctaccagaa
    11881 tgactttcag cttcaatggc atcgaaggca cagagacggg gagagggcgc gactcatcaa
    11941 acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc cacagcagag
    12001 gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc tgatgcacca
    12061 gacctggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag agttgttccg
    12121 cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca tctgcactca
    12181 ctgcgttacg taagcggatg gtgtgcccgg aaacctcata gtgaccgccc accagttggc
    12241 ctgcatcgct ttgtagcgca cgcgcggcat cggcaataag attcagatac tcagactctt
    12301 ccggggcctt cgccagcata aaagaggagg atgctcgcgt atgcagcaac tgctccagcg
    12361 caaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca atcaaatgtg
    12421 gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc catgcaagtt
    12481 tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt ataccgtaac
    12541 cggcactaca gcacacgtag aaagcaccga caatactcct ggcatgggcg ttaaagctca
    12601 caggatggag attcttttct tcactggccc aaaaagctga tattctgtaa agagttacac
    12661 ggtaacattg agatcgctat gaaatatcaa caacttgaaa atcttgaaag cggttggaaa
    12721 tggaagtatc tggtgaagaa gcaccgcgaa ggggagttaa tcacccgtta catagaagcc
    12781 agtgccgccc aggaagccgt cgatgtgttg ctctcgctgg aaaatgaacc cgtgctggta
    12841 aatggctgga ttgacaagca tatgaatccg gaactggtca atcgcatgaa gcaaaccatt
    12901 cgggccagac ggaagcgcca ttttaatgcg gaacatcagc atacgcgcaa aaaatcgatc
    12961 gatctggaat ttattgtctg gcaacgtctg gctggtcttg cgcagcgtcg cggtaaaacg
    13021 ctttctgaaa cgattgttca gctgattgaa gatgcggaaa acaaagagaa atacgcgaat
    13081 aaaatgtctt ctttgaagca ggatctgcag gcattgctgg gtaaggaata actgacgaaa
    13141 gtcagttcaa ttttctaaag acgaaaaaaa accccgcagc agcggggttt tttaccagac
    13201 gagaacttaa gcctgcggct gagttacaac gtctttgatg cctttaactt cgatctctac
    13261 gcgacgatcc ggagccaggc aatcgatcag tgcagcacgc tgtttcacgt tgtcacaggt
    13321 gttgccagta accgggttgg attcgcccat accacgtgcg gagattttgt ctgccgggat
    13381 acctttagag atcaggtaat caacaacgga ctgagcacga cgctcggaca gagcctggtt
    13441 ataagcgtca gaaccgatgc ggtcagtgta acccagaaca actacggaac cgtctttcgg
    13501 atccaggttg ctcagctggc tgtacagctg atccagagca gcctgacctt ccggtttcag
    13561 ggttgctttg ttgaaggtga acagaacgtc agacttcaga gtgaagtgct tggtctgtac
    13621 ttccggtgcc ggagctggag ccggagcaac tactggagct gcttcgccct gaccgaaacg
    13681 gtaggaaaca cccaggctca gcatgccgtt gtccggacga gtgccgatgg tgtgtgcgtc
    13741 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcata
    13801 ctcaacaccg ccagcgaaga ccggagaaac gccggtgtcg tggtctttag tggatgcgcc
    13861 accaggtacg ttagacttgg tgtctgcacg ccataccata ccacccagac gagtgtaaac
    13921 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    13981 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    14041 ttcaaagcca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    14101 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    14161 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    14221 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    14281 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccggggatct
    14341 gctcaatatt aactctaccg atatcttcgg cttatgccga gcacccctgg cgatgtaaag
    14401 tctacaacgt agttgaaaac ttacaagtgt gaactccgtc aggcatatga aaaaaaagtc
    14461 ttgtataagg tacgtttaat cttttttgtc agcgacaatt tacagaagaa aatcgcggaa
    14521 accgcttcag acaagcctcc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    14581 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    14641 ttagcccgga aagttgtctc gttgcgtgag aggatgcgct taccggacgc ataataaacc
    14701 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    14761 catctgccaa ccaaccgatc accacgctgt aattgcccgt gcgtaaagcg cgaaccattg
    14821 actcaacggt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    14881 cagatgcctg aacccattcg cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    14941 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    15001 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    15061 gcgcaatttt acttgctgcg gatgagaacg acgaagcacg atgtgcatag cctgaagtgt
    15121 acataatcaa tccagccctg tgggttactg tatgaatata cagtacatcc ggtgacgaca
    15181 aagatcaacc ctattttcgg aaagagcctc gcaaattttg tcgttagtga cgggaaaaca
    15241 taaattaatc ttgcccctta agaataagtt gcctattttc gtagttaacg gatccgttaa
    15301 tgtgaatcat tcttttatgt tatgatttta aaaggaattt tatgaaaagc ctctcctata
    15361 agcggatcta taaatcacaa gaatacctgg caacgttggg cacaattgaa taccgatcat
    15421 tgtttggcag ttacagcctg accgttgacg acacggtgtt tgcgatggtt tctgatggtg
    15481 agttgtatct tcgggcttgt gagcaaagtg cacagtactg tgtaaaacat ccgcctgtct
    15541 ggctgacata taaaaagtgt ggccgatccg ttaccctcaa ttactatcgg gttgatgaaa
    15601 gtctatggcg aaatcaactg aagctggtgc gtctgtcgaa atattctctc gatgctgcgc
    15661 tgaaagagaa aagcacgcgc aatacccggg aaagactgaa agatttgccc aatatgtctt
    15721 ttcatctgga agcgattctc ggggaggtgg ggattaagga tgtacgggcg ttacgtatac
    15781 ttggggcaaa aatgtgttgg ttgcgactgc ggcagcaaaa cagtctggtg acagaaaaga
    15841 ttctgtttat gcttgaaggg gccattatcg gcattcatga agctgcgctc ccggtggcac
    15901 gccgccagga gcttgcagaa tgggctgact ctcttacgcc gaaacaggag tttcctgcgg
    15961 aacttgagta atctggcgtt gcagacgacc aatctctggc agtaatgcaa tcaataaccc
    16021 aacttgttgt acgaccagag gttctttgct gtctgcccgt ggttcaagtt gctgcatccg
    16081 ctgtttcagg ccagctaatg cctgattgac gcgttcttca tcagcaggtt gatgatgtaa
    16141 cgcgtcatca acatagcaaa ctgcgtcatc aagaaacgcc agaatttcag gattagttaa
    16201 ctgctcccgg tgagcaccga gggctgagat atagctggta aacgtatggt taaggcacag
    16261 caaccgaaac gcggcttcgc gaatttgcgg ggtaacgttc ggttcgctgg acatatttga
    16321 taccaccgac gccagttcag catcacggtt gtgtgcatcg cggcgggcaa tacgatacgc
    16381 cagacggtta tcacgcccct gatggtattg ctccagtatg gcatcgagat accggcagtt
    16441 ggcctcggtg gcgcgttcga gcattcgcgg cagattgcga aacttccagt caggccagat
    16501 gtagctcact gccgcccacg caatggcaca accaatcagc gtatcgatta cgcgaggtaa
    16561 cgccacttca aaaccttcac ccagcaagtt gaaacacagt agcaccaaaa gtgtgatgaa
    16621 catcgttgca tgggcgtatt gcacgttacg gaaggcaaaa aagagcacgc cggtaataac
    16681 cagcagcacc agctgccctt ccagtgatgg cacaaaccac agcacaggaa taccaatcgc
    16741 gatacctacc agcgtaccaa taatccttaa cttcaggcgg tggcgcgtgg cgttatagtt
    16801 tggctggcag acaaacaaac ttgtcagcaa gatccaatac ccgtgatgca ttccggttat
    16861 ctgaatgatg gcgtagccga agcacaacac cagcgacatt cttaccgcat gacggaagag
    16921 ggcggattcc ggcgtgaagt gacggctaag acgcagccag atatcactca atccgtgcgg
    16981 gctgtcatca gcgagctcat tttcgtcatt attatggggc agtgcctggg cctgttctga
    17041 ttcaattgtt gccagttggg catcaatggc acgtaaattg ttcagtaaaa atcctagtgt
    17101 tttgagtaaa tcagcaggag cgccgttatc gcgcatccgc tccagcgcag catcaatatg
    17161 cgtaaaagcg cgctcaaaat gcggatcatg ttgataaggc tgacgcagca aaatacagcg
    17221 tgacagttgc tggcacgcct ggccctgcat cgacatcagc cgctgaaaac ggaacagcac
    17281 gtcgctgtgg cgaaaatgtt cacgcaatgt ttgatattga atatgagaag agctggcacg
    17341 ctcgtgaata tcctgcgcga caaagtaata atgcagtgtg cgacgcgttc ctcgttgacc
    17401 acgatcgcca cgtaagcggg tcagcagcga gagtttcgtc tgattcaatg tcgccatcag
    17461 ctgaccgttg gcgagagcca aatcgtacag cggtgcctgg ctttcatctt caatatcagg
    17521 atcaaacatg cgcgacttga gctcaagata acgcgccagt tgttcatagc aacgcgccag
    17581 gttgtcctgc agcgggcgga ccgggaacag cagatgacca ataagtgtca ggacgttgta
    17641 ccagacggca ccggccagca gatacatcgg ctgctgatac cagtgctcat acagtgacgt
    17701 ccccaacata gtgtaaatgg cgatcagtaa cgcaccgaag gcaattgttg catagcgttg
    17761 acccagaccg ccgagcaaaa tgaagccgct ggtagagagc gttaaaccaa ttgcaaatag
    17821 ccagggccag ggaaacagca attctactga ggccgaggcg ataaaaaagc agaacagcgt
    17881 aatgatgagg ttacgcaaac gtcccgccag tcggtcatcg agatcggtca gcgccgctgc
    17941 caccatcccc agcgttagcg gaatcgtcag ttttacatca cccagccacc acggaaacgc
    18001 tgtggttcca caaagcgcaa taaaaatacg cgcgtaatac agccaggcgc tgttccaggt
    18061 atagcgtttg agcaaaggac ttagcataaa ggccatatta gtggattatt caaaacgacg
    18121 acgtgcattg gcttcacgcg cagcttgcgc tgtttctacc gataccacgc gacgaccgac
    18181 cggccatagt gcaatagcgg caattttaaa gttcgcaatg ccgacaggaa tgccaatgat
    18241 ggaaatacat tgtgcgatgc ccgttgcaat gtgcatcagg cataaccacc agccaaagaa
    18301 aatcagccag aaaatattca ataccgtacc gccagtattt agcagcacat ttttgccagc
    18361 cgggttcagt tcatcgacat gaatagcttc attgccataa ggcaccagag acagtttagt
    18421 gatctcccag caggatcgtg tcagcggtaa ggtaaaaatc agcacaatac tgaccagagt
    18481 cgccaacagc cagcccagag tggtggcaaa tccgccaagc acaaagttca gaatgttcaa
    18541 aacggtacgc ataaaacctc gctttactgt ggttttcagt aatgggcggc aattgtaacg
    18601 tttttttggg ctggagcacg ttttctctga cggttacact gataagaaat aatttcgtgt
    18661 ggatctacag agtcatggaa ctgaaagcga caacgcttgg aaaacgtctg gcacagcacc
    18721 cttacgatcg ggcggtgatc ctcaatgccg ggattaaagt ctccggcgat cgccacgaat
    18781 accttattcc tttcaatcaa ttactggcga ttcactgtaa gcgcggtctg gtatggggcg
    18841 agctggaatt tgtactgccg ggcgaaaaag tggtgcgtct gcacggtacc gaatggggcg
    18901 agacgcagcg tttttaccat catcttgatg ctcactggca gcggtggagt agcgagatga
    18961 gcgaaattgc atctggtgtt ttacgccagc aactggattt gattgccacg cgtactggag
    19021 aaaataaatg gctgacgcgt gagcaaacct ctggcgttca gcaacaaatc cgccaggctt
    19081 tgtcggcgtt gccgttgccg gttaaccgac tggaagaatt cgataactgc cgtgaggcat
    19141 ggcgtaaatg tcaggcctgg ttgaaagata ttgaaagcgc tcggttgcag cataaccagg
    19201 cgtataccga agccatgctt accgagtatg cggatttttt ccgccaggtc gagtcttcac
    19261 cgctgaatcc ggcgcaggcc cgggcagtcg ttaatggcga gcattctctg ttagtgctgg
    19321 ctggtgcagg aagcggaaaa acgtcggtgc tggtggcccg tgcaggctgg ttgctggcgc
    19381 gtggtgaagc gtcccctgag caaattttat tgctggcgtt tggtcgcaaa gccgctgaag
    19441 agatggacga gcgtattcga gaacgactac ataccgaaga cattaccgca cgcacatttc
    19501 atgcgctggc gctgcatatt attcagcagg gcagcaaaaa agttccgata gtcagcaaac
    19561 tggaaaatga taccgctgcc cgtcatgaac tttttattgc tgagtggcgc aagcaatgca
    19621 gcgaaaagaa agcgcaggcg aagggctggc ggcaatggct gacggaagaa atgcagtggt
    19681 cagtgccaga aggtaatttc tgggatgatg aaaaattaca gcgtcgtctt gcctctcgcc
    19741 tcgatcgctg ggtaagtctg atgcggatgc acggtggtgc acaggcagaa atgattgcca
    19801 gtgcacccga agtgattcgc gatctgttca gtaaacgtat caagttgatg gccccgttat
    19861 taaaagcctg gaaaggtgcg ctgaaggcag aaaacgctgt cgatttttcg ggccttattc
    19921 accaggcgat tgtgattctg gagaaaggtc gctttatcag cccgtggaag catattctgg
    19981 ttgatgaatt tcaggatatc tcgccgcagc gcgcagcgtt gttagcggca ttacgcaagc
    20041 aaaacagtca gacgacgttg ttcgccgttg gtgatgactg gcaggcgatt tatcgcttca
    20101 gcggtgcgca aatgtcgctc accaccgctt tccatgaaaa ctttggtgaa ggtgatcgct
    20161 gcgatttaga cacgacttac cgttttaaca gtcgtatcgg tgaggtggca aaccggttta
    20221 ttcagcagaa cccaggccag ctgaaaaagc cgctaaacag cttaaccaat ggagacaaaa
    20281 aagccgtcac gttattggat gagagccatc ttgacgcttt gctggataag ctctctggtt
    20341 atgccaaacc ggaagagcgc attctgatcc tggcgcgtta ccatcacatg aggcctgcca
    20401 gcctggaaaa agcggcaaca cgctggccga agttgcaaat cgactttatg accattcatg
    20461 ccagcaaagg gcaacaggcg gattacgtca tcatcgttgg cttgcaggag gggagtgatg
    20521 gttttccggc tgcggcgcgg gagtcgatta tggaagaggc gctactgcca tcggttgagg
    20581 atttcccgga cgctgaagaa cggcggttaa tgtacgtggc gctgacccgg gcacgccatc
    20641 gggtatgggc actgtttaac aaagagaatc cctctccatt tgtcgaaata ctgaaaaatc
    20701 ttgatgtgcc ggtggcgaga aaaccgtaag aaacaggtgg cgtttgccac ctgtgcaata
    20761 ttacttcaga cggtccgcga gataacgctg ataatcgggg atcagaatat cgaccgcgtc
    20821 gttgaaatgc ggcgactgga taatgaagtc tgccgttgcc acgttggtgg cgaccggaat
    20881 gttccatacc gtcgccagac gcagcaaggc tttcacgtca gggtcgtgcg gcacggcgtt
    20941 tagtgggtcc cagaagaaaa tcaatacatc aattttccct tctgagatca atgcgccaac
    21001 ctgctggtca ccccccattg ggccactcaa catcgcgttg acgttcatgc cggttgcacg
    21061 ggaaattaag ttaccggtag tgcctgttgc atacagtacg tgttgttcca gtaacggttg
    21121 atgccgttcc acccagctca tcagcatttg tttgcagtga tcgtgtgcca ccagcgcaat
    21181 atgtttccgc gcaggtaaag tgcgtgtcgt cagttccata atgtacatcc gtagttaact
    21241 ttcctacaga ttactgtaag cacttatcgc tgcaagataa agaccgaaaa agcctgcgca
    21301 caggcacaaa aatctcagga agatggttgt ttttccgccc attgcaggaa agtatttcgc
    21361 gtttgtgggt cagccagttt aaaccaatac ttcagccgtt gttctgtgag cacctgagac
    21421 tgcggtggaa tagcatccag ctcggaaaca cccgaaagca gcgtactatc atctgccatc
    21481 atcgtggcaa attgcggtag tgaagcgcgt ttcgcggatt tgttatagcg ttcaacctcg
    21541 acctcataat caatcgtttt tgccgttgag gtaatggcga gaatatccag ctttaccgga
    21601 atcggcgtcg catcgccatc caacaattca aggcgcggcg cggcatcaaa atggttagct
    21661 tcccgctcat tctccagacg aggcaggcga aaattgacct ggttgatgag ctgggtatta
    21721 aaactgacca ccagcggtgg ggagatatag agccgttctt cactattgga aagatgaatc
    21781 gtcttctcaa cgcgaaacac taactgatgc ggtccgttat ccagttcaat gctgtccgca
    21841 ccgcgcagca gagagctgga aaccttcttg ccgtcgagca ccagaagatc gacatcggtt
    21901 gaaagccgca gcgtggtggc aaaaacggat accggcaaac ataatgcaat caaggtggtc
    21961 acgatgccgg ttttcatagg tagctcctgt caaaagaccg cccgaataat aatgtgtcaa
    22021 aatttttctt taatcatact tactaacata tagacatatt tcccgctttt gctctcattc
    22081 attcgtatta gctgcatggt tggcatgtcg gctttgtcgt acactttgtt aaacagtctg
    22141 gaggaaataa tgaaagaaac cgatattgca ggcattttaa cgtccactca caccattgcg
    22201 ctggtggggg caagcgacaa acccgatcgt cccagctatc gcgtgatgaa atatctactc
    22261 gaccaggggt atcacgtcat tccggtttcg ccaaaagttg ctggcaaaac actgctgggc
    22321 cagcaggggt atggcacgct ggcagaagtt cctgaaaaag tcgatatggt cgatgttttc
    22381 cggaattcag aagcggcgtg gggcgtagcg caggaagcga tcgccatcgg cgcgaaaacc
    22441 ctctggatgc aactgggggt gattaatgag caagcggcag tgctggctcg ggatgccgga
    22501 ttaaatgtag tgatggatcg ctgcccggct attgagatcc ctcgcctggg cctggccaaa
    22561 taaaaaatcc ccggaaggca aaaaccttcc ggggatttgt tcagggatta gttacgcaga
    22621 cgcggggcct ggagttgttt acggatggtc tgcgccagct catccatgga tggctgttca
    22681 ggatgttcat cctgcaattc gctacttagc tgggcttcag ccaggtaggt atgaaccggt
    22741 aggccgttat cgtcctccat caccacgtga taccacggag cggcgcgaag ctcgtcattc
    22801 accgccagct catcaggcga cggttcagag agcgaataaa ccgggtcgat atccacgacc
    22861 actccgagat aacctaacag ggaatggcgg acctgctggc cgataccgaa tttgctggca
    22921 atcatagtca cctcccggga aatctttacc tttgatataa gggtaaaatt ccacatttca
    22981 agttacatga cgcgacaggc aaaccctttc agatatagcc cttccggata ggtagcgatc
    23041 accggatgat cggctgcctg acggaactgc tctataaatt gtacatcacg accggcatca
    23101 attgcggcat ccgcgatgat tttctgaaat aaatcgctgg tcatcagacc ggaacaggag
    23161 aaagtcagga gaataccgcc ttcattcagc agctgaatcg ccagcatgtt gatgtcttta
    23221 taaccacgac acgcgcccat caactggctt ttattctcaa caaacttcgg cgggtccatc
    23281 acgataacgt caaatttttc accgcgatcg cgataagtac gcagcaattt aaagacatca
    23341 tcacggacaa actcagcctt gctcagatcc agtttgttca gctcaacgtt ctgccgtgca
    23401 atatccaggg cttcctggga ggtatcaacg ctgacaacct ggctgcaacc gcccatcagt
    23461 gccgatacgg cgaaaccacc ggtataggag aaacagttaa gtacgcgttt attttcgacg
    23521 tagcggcggg tagccaggcg gctgtcacgc tgatccaggt agtagcccgt tttgtgtccg
    23581 tgctgaatat tcaccagcag tttcatgccg tgttcttcaa tcggcagcag ggcaggcggc
    23641 aactcgccgg tgatggggcc ctgagtcagc tccatccctt cttttttacg taccgcgacg
    23701 tcgctgcgat cgtaaatggc acattccggg tacagcgttt gcagggcgct aattaatgcc
    23761 gcgcgctgat actctgcgcc agcactcagc agttgcagca ccagaaaatt accgaaacga
    23821 tcgatagtaa tacccggcag gccatcagat tctccggcga ttaaacgata gctgtcgagg
    23881 ccatcttttt gcgccagcca gtcacgccat ttttgtgctt gctgcaaacg gcgggtaaaa
    23941 aaagcaatgt cgatagattc attcgggtca aacgtccaga cgcgcgcccg gatttgcgaa
    24001 gctggcgaat aagcgccgcg tgctaaccat tttccctgat gatcaacaat atcgatggtt
    24061 tcaccgaggc tggctttacc ttccatgcgg gcaacggccc cggaaaagac ccacggatgg
    24121 cgacgaagta atgatttttc gcgccctttg gctaacacta aacgtacact cataatttac
    24181 ttttctgtcg atgcaaagaa atgggcgtca ttgtccggag ttttccatgg atttgcaacg
    24241 cgtccattaa ggataaggag agcagcaatg tcgaaagtct gcataattgc ctgggtttac
    24301 ggtcgagttc agggcgttgg atttcgctat accacgcagt acgaagcgaa aaaactggga
    24361 ttagccgggt acgccaaaaa tcttgacgat ggcagcgtgg aagtggttgc ctgcggtgaa
    24421 gaagggcagg tagaaaaatt aatgcagtgg ttaaagagtg gcggcccgcg ttctgcgcgt
    24481 gtagaacggg tgctcagcga gccgcatcat ccctcggggg aattaaccga ttttcgtatc
    24541 cattaaatac attttaccgg ttttggcagg ccagcaattt tggtggcttg cttagccgga
    24601 cctttcggaa acagtcggta taaatagcgg ctgtttcctt tttcttcgcc aaatttattc
    24661 gccatcgctt ttaccagcat acgaatcgcc ggagaagtat tgaattccag atagaaatca
    24721 cgcacaaaac gcaccacttc ccagtgttct ggcgacagcg taatcccttc gttctctgca
    24781 atcaccactg ccagcggctc actccactgg ctgctttctt tgagatagcc ttcggtatcc
    24841 gtttctatct ctttaccttc gaagatcagc ataattatta ctaccttaat cagactggcg
    24901 gcagtgtaac aaaaaacaaa gccccgcata aagcgaggct atgaaagtgt tagcgggtaa
    24961 gattaatcgc ggctggcgaa gcccagaatg ctcagcaggc tgacgaagat gttgtacagc
    25021 gaaacataca ggctaaccgt ggcacgaata tagttcgttt caccgccatg aatgatgttg
    25081 ctggtttcaa acaagatagc gccagaggag atcagaatga agaccgcgct gatcgccaga
    25141 tgcagagcag gcagttgcag gaagatatta gcaaccatac caatcagcac caccacaata
    25201 cccgccatca gcataccgcc gaggaacgac atatctttgc gggtggtcag cacatatgcg
    25261 gagcagcaga agaacactaa cgccgttccg cccagtgcca tagcgattac gtcacccatt
    25321 ccggcagaca gataggtatt cagaattggt ccgaggatat aacccagaaa accggtaaag
    25381 gcgaatgcgg agataatccc ggtcggctta ttcgccgttt tataggtcag gaacatcaaa
    25441 ccatacatac ccaccagcgt cagaatcaga cccggagatg gcagcatcag caccgtactg
    25501 gcagttgcgg taatcgccga aaaggccagc gtcaggctca gcagaaaata ggtattacgc
    25561 agcaccttat gggtgctaag cagcgatgta cggtcatgtg aagaactaac aatacgatcc
    25621 attgagtcac tctctatgac agatgtaatt aattaagcag cataatgata atgcgtaagg
    25681 gcacccagaa gttttaccca tctttacgca tttgatctgg aacaggttta acagcggatt
    25741 atcaggttat taagcaatga tataacgctc tgagaatttc gacagacaaa agaaaaaggg
    25801 gttagcattt agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga
    25861 accctttcgg gtcgccggtt ttcaagaccg atgaaaaata aataaaaatc aatgcaatac
    25921 gaaaaatagc tgaaaaatta accaaactta acacctagaa aaatcaggct gttagggagc
    25981 tttcagagtt agtataccaa gtcttttttg ccagaaaatt tcctgagttg cctacttgct
    26041 gaccacaagt ggcaaatcca gtgtcggcgt gatttttgtt ttacgatcat aaatcaacac
    26101 ctgattttct gttttatgtc cactgaaaat ctgtttgtcg cgactgcttc cttcgtaatc
    26161 tgatattcct ttggctttta tgtcatggaa attgcaccca aacgggacgc cggatttttt
    26221 ctcggctgcg tgtttagcct ggttccacca gttgtttagc gttttagcta tgaccttccc
    26281 gcccttggtt gtgttgatca catattcaca tgtgcctgaa gatacatttc tggctaactg
    26341 gatcgcggtt cgtaatcgtg gagaccattc cttgatttgt ttggtgccgg tcttgttttg
    26401 ctcaatgtag attcctttat ccataatatc ctgccatttc agctcgagca catcaccgag
    26461 tcttgccgca caaagatagg atatctccat tgcaatgcgt aactgtggta ttgcttccgc
    26521 atatatcgcc gcatactgtt catctgtgat gtaaacagtg cgggctttaa gcgtaaattt
    26581 cctgactcct ttgcatgggt tattcttcac atatccacgc tcatatcccc acccgtatac
    26641 acgactgaga cttgccagtt catggtttgc ctgggtcttg ctctcaatcc cccgtttatc
    26701 catgaaaatt cttacctgct caatttttac attatcagcc agtacttttc cgaagactgc
    26761 cagtaacgcc ctctgatgtt gccgataatc tttttgggtt ctgggggcca gttctgtaaa
    26821 tgcgggggaa tccataaaca tgtgccataa tttagcgacg gtcattatgt tgtggcgttt
    26881 tgctttttcc agttcataat tctgccagac tttagctatg ctggtttccc gcactcttcc
    26941 tagccctata gtttttgtgc ttccttcggg tttccatacg taactgtaac cattcgatct
    27001 aacacgtggc ggtagtgcat tatctttttt gttttttctt ggtcttccca ttgttcagcg
    27061 cctcaaaatc ggggtcagca gaaaccagtt cagatacttt cggtatcgca gtcagtccgc
    27121 gcgggacatc actgcgaaga actattggtt cgtttttagg accgggtaca aatgggatac
    27181 cgtgcaacct taactgttgt tgctgttttg tataccgttc gtatctcgtg atctcttgaa
    27241 tttctgctgg cgatagagtt aattcgtaca tgtggtcacg tgccttacag catgaccgcc
    27301 gccactatag cttgtgggcg gcgatatggg ttgaacatta aaaatcagcc tgattcggga
    27361 acagtttttg ccagatagct gaaacgtatt ttgcctggta acgcgcatcg tgcagggcat
    27421 tatggcgttc accctcaaat gtgatgacac ttcgagcatc gaaatccatc accagcccca
    27481 gggcaacaat cgttcttaca tcacgatcat tggtgtaacg ccacgggcag gggatctcct
    27541 gccgttcata tgaacggcgt aaaatcacgt tgtcgaagtt ggctccattt ccccagacct
    27601 gaacaaaaaa ctcaccggag ttttcgtcta taaattcccg taattgtaaa agcgcatcat
    27661 caagcggtat tcgatcagct aaaatggcgg cacgggcttc gccagagcgt tgaagccacc
    27721 attcgatggt gcttatgtct acagtgccgc cacatgtacc catatcaatt attttgctga
    27781 attcaggccc ctgctctccg gtttgtggat cgaaaaatac agcgcctatt gaggctattg
    27841 gtgcgtcagg atttttgccc attgtttcaa ggtcaatcat cagatggtgc cataacctgc
    27901 tggtggatgt gatttcacga tgaccgttca ccttaattaa gggatttgct gtctcgcctg
    27961 tttcattatt gccattggca tgttgatcgc cttcggtgca ctccttgttc gggagtccgg
    28021 cactttccat ttcctccgga tctttttccc gggcttcatc ctggctttct tcgttgaatg
    28081 tctcctggta ggttgcgtcc cccatcactg cgccacaatc agggcagtta ccaccaccag
    28141 tctgattgca tgcggtacaa actttttcct cttcctgttg cactactggt tcaggttgtt
    28201 tcgtttctgg ctcgttttgt aacgcatttg ggctgttttg ttccgctttc tgaccgttct
    28261 gttccgtttc ttgctggttc tggttcacag aatcgcaggt ttcaatcccc ttaacccatt
    28321 tcgaatcatt cgggtcgcta atcccttcaa caaactcacc acgagaagcg gcaagcaatt
    28381 tgtcggcatc tggtcgactg atgtctgctg cctccataat tttatttact tcttcagcag
    28441 taacttttac ctgtgacttg tcatccagcg gctgcgggtc ctgatgatgt tcaactgtat
    28501 ccgctgccat tgtttcagcc gttgcctgtt catctgccat tgcgccagat ggttgcgggt
    28561 tttcttcatc atttttttct tcttctgttt tgcactcagc agccagtccg ccgttaattt
    28621 cttccaggat atctttttcc ggtgtatgcc gggcagccat gagcgtttca gctgtgggat
    28681 tctcgtgatc agtttctgtc aggttggcat tgatataccc ctgaaggcgt cccgggtagt
    28741 gataaaactc agggtgggcg ctccggataa gtgcaaaaat agctgcgcgg gaatagtcca
    28801 ggataccagg ggttgcgcga agtgctgcgg accattccct gaacggactt tcttttttca
    28861 ggacgatttc ttttgcgcga cgataaacgc tcacgggtat ttcataaata ttaaaatcca
    28921 tcggaagtgt ggctgctgca gcctccacat ccagagtatc gagggtgtgt actaagtcag
    28981 gattacgatc ggttttattt ccgccaccgg catttgcacc ggaagccgtg cgggtgatgc
    29041 gagaaacacg atttcctttc atccactctt ttgtcagcag acctcgatcg gtatagtcag
    29101 cgtccaggta tgcttcgaaa aaagcagtca ttaaccccag actggaatta acgggattag
    29161 ggaaaacttt gtctgtgtcg cgtaccagtt tatgaaggtc gcgaatctcc agcgggtcga
    29221 gcagctttgt gttgtgggaa atagccaggg cagtaacagc cggcagttct tcagctcgtg
    29281 cgatatgtaa tgcctgaagt tcttcccgtg aaacgtgcgt taccggtttt tcgctgccat
    29341 gttgcgcaag ccagcgaatg ggcagttcct gaccggaaat cggcaggagc atattatcct
    29401 caatctcagc catgtcttcg ccattgacgt tggtattgtc agtgctggct ggtttgtcct
    29461 gaactgaggg ggaaggcgct ataaatatca ttgtgatgcc atcttccccg cctttttcat
    29521 agcggttgca gaattcggta tcaaacacgc cttccggtgg aaggtcattc acaacgggga
    29581 aatggacgcg gacaggtttt ttgaaatcat cagcgtcgta gcctgcctcg tccattgcaa
    29641 caacgcaccg tgagatggca acagataatt tttttgctgt agtccagtaa aaacctcctt
    29701 taatcccaag gcgttttctg accttgtcat tttttgcttc gcaatatagc gaaaattctt
    29761 ctttatcagt gctcatggat aaacctcata actattttaa ggttgtacga acccctgcca
    29821 ttgctggcat gcttaatcaa cgggtatggc gttaatatgg ctggtggatt atccagccgg
    29881 tatttcgtta ttcaggtaca gcgatacttt ttttaacggg aggcattcgc cagaaatttt
    29941 ttgctcgtct cttgcacgga ggcatgattc tttactggca taaattccgg taatcacatt
    30001 ctgtggctca cccgttataa gaaaaaccgt catcaccagt gcaaatgcag aagtcactgc
    30061 tgccctccga aaataccaag tttaagaagg gcaattctgg agagtatgga attatcattg
    30121 agaagataag gctcatattt tctcatctta atggcatctt cagtaaactc ccggttactg
    30181 agcagaatac caatatcaaa acacccttca gacgtattaa cgtttggtaa taacgtttcc
    30241 attatcgcgt cctcaacaat gaatttttag taattgctcc acagtcatat ttttaattgc
    30301 gctccggttt acaagagtcc agccttgttt ctccagataa aactggaagg tatccagggt
    30361 gcagaccatt gcaccatcag gaacggtttc agtgaatttg atattgccgt gttcgtcgag
    30421 acggataacc agggtacgtc cgtccccgtg aatcattttg tcgggagacg gggcgttatt
    30481 ctggcgcagt tctttctcca tgcgatcgaa ctcagcgatg taggcttctt taaatgcagc
    30541 ggcttttttg ccggtgaagc ccatcaccag gaaaacgaag ccgtttttgg tgatttggta
    30601 catagggagt ttgcgcccgg ttgagtcggt atattcactg ggcgaaaaat ttcgctcagt
    30661 gaattctgct gaacagtcta gacgtgaaat ttttgccagt acatctttgt gtggtttccg
    30721 gaaatactcg gcaacagcta aagaggatgt aacagcacgc ccattggcga tagtaatttc
    30781 agggtgagaa agggcaggga tagtagccat gatggcagcc tccatgatga atttcattaa
    30841 ctcaccaccg gaggtagcaa tctcatgggt ggtgagacgc acagggttgc tacaaccggt
    30901 catcatggaa cccggccagc cttgcggctg ccctgcacgc cccaccataa tctgaatgtg
    30961 gctgtgcatt acgcataaaa aaaccgcctg agcgcggtta tgcgccatga tgaacatcgg
    31021 ggtagcaatc ccggcacccg ctttatgagg tgcaggtgca ctataattcc acccgttctg
    31081 gttttcaata gctacattca acattttctc ttacctttca tcaccgaagt gaactttgtc
    31141 gatgcagtgc ctggtgcctc caggtgacgt taaccagtta acaattaacg ccggaataca
    31201 gaagtatgcc cgttacgccc cgtaaaagac cactttacgg ttttaactgt tccgcgtgcg
    31261 cttagccgca ttcaccgcat cacaaaattc actttaaaaa gggcggacat cagaaagcta
    31321 ataaactgat gccgccaaag gtaccactgc cattgaaaca tggagatgtt gtggcggggt
    31381 tgtcactcag gcatatggtc aacctgacaa cccggtgtcc tcaatgaggg gaaaagaaat
    31441 aaaaaccccc agccatattt accgccgcgc catttcgcgg attgccacaa ccggaagcac
    31501 acggtcgaag aaatctaacg acaagccttc taaggaaaag acgccttcgc cgtgtgcttt
    31561 ctgttgtgcc ctgacttttc agggtaaatt aaccagtggc aacctgtttt tactggtgct
    31621 aatcagttag tgtttctggc taaccagcga cgcgcgccag cttcggtttt aaacttttta
    31681 cttttggtat acgtcatcgc ggtaaacgtg ccgtcctggt tagggaacac accgcatacc
    31741 agagattcgt tgttgccaag attgagcgta tccatgttga cctcatttac ccttaacgcc
    31801 gggtcgcgga actaaaaacc tgctgcgctg ttatataaag tgttcccgcc gtcatgttca
    31861 tacgcctcgg gctggctact taacccctga ccactgccgg gtaactcgaa gtattgcctg
    31921 gcgttctgtg gggcagggtg ggttggtgaa attagtttat acaaaaataa actaaacagt
    31981 caatatatag cttaaccgat gggcgggaat aaccctccct cttgagaggg ggaaggtttt
    32041 gctttagggg gattaaggtg ttacagggaa ctgtcggcgg gcgtgaacta cgttaacaat
    32101 ttcaatgcct gaaattgtca ctcggtacag aataatgtaa ttggggtgag ctacaatttc
    32161 acgcaaacct ggcactctgt cacttggcgg gtataaatac ggatgctcag ataatggctg
    32221 aacgcaagtc cttaagcgtt tccataaacg ttctgccgca tctatgtcaa agcgtgcaat
    32281 atagctggtt atatcatcca agtcggtatc tgcgctttca agccataaca cgggtaacat
    32341 ttactgctca ctccgttcct tgcgcatttt agcaaagcgt tctgccattc tacgctcaac
    32401 ctcgtcatgg ggaattgctg ggcgcggatc tgcaaggctc gctgctactt tcgcacgcag
    32461 ccattcgttg taactgtttt cttgctcgac agtttcaaat tcagaaacca tcggtgaaag
    32521 ggctgtgttc atggtgaacc tcgttttgta agtgctggta ttctgcttgg ttgacttttt
    32581 gttgtgctgc taaccacctt gcaactatac gatccattgc ttcttttttc tccttcatat
    32641 ctttaagcat ttgctgttga tcttcctctg gaaatgcctc aaatgtttga agtagttcct
    32701 tttgattagg ggttattttt aatatatcct gtgccctgtc tttttttctt catttaatgg
    32761 taagaaaaac caatactgag gaagtcccgt tacttcagat agtttatcta gattagctgc
    32821 cgtagggctt gagataccac aaacccaccg ttgcacggat tgttgagcaa taccaattct
    32881 gcgcgcaagt tctgcttggc tccagccttt tgccaataga agcatcttaa ttctaagatg
    32941 cactaatttc gagtacgccc atctgatatc tatatatccc gtcaggctcg gcgaccttgt
    33001 cgtgccaacc aacgtgcgac aagatcttcc atagcctctt ttttgttttt tagttctttt
    33061 atgatctcct gctgctcatt atcagggaaa gcactaaata tttgtagcaa ttctaattgg
    33121 ttaggggtta atgctataga tcttgatgat ggagactctg ttatgtcttc tggttcaata
    33181 tcaagatatc ccccaggcat cccatatgtt tgttcaatac gtctagctgc cttttcgcca
    33241 aacgtagctt taccgctcat taactgagat aagtaacttt tctctttagg cggcaaagtc
    33301 ctgtctttga accactcttt gaggcgtaaa cgccgaatct cttttgtttc catacataca
    33361 ttatct
//
LOCUS       DAZUCC010000028        33208 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZUCC010000028 DAZUCC010000000
VERSION     DAZUCC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.028"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..215
                     /locus_tag="ACHY09_001187"
     CDS             <1..215
                     /locus_tag="ACHY09_001187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884188.1"
                     /translation="CRIPEDPHHWNGFSGEKRILYGQSAHHFFFSSSTIHVSLPRLIT
                     DYEMQKYLSFQLPVIRYRERFWQYME"
     gene            222..2645
                     /gene="pgaA"
                     /locus_tag="ACHY09_001188"
     CDS             222..2645
                     /gene="pgaA"
                     /locus_tag="ACHY09_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFS3884189.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYSRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLKS
                     GAPDKANLLAEAYIYKLTGRHQDELRAMTESLPENASKQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELIRLSFMPTRSESERYAIADRALAQYAALEILWHDNSDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKNTRQIIPPWAQYWVASAYLKEQQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMIARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLVELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRQEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2654..4672
                     /gene="pgaB"
                     /locus_tag="ACHY09_001189"
     CDS             2654..4672
                     /gene="pgaB"
                     /locus_tag="ACHY09_001189"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFS3884190.1"
                     /translation="MLRNGNKYILMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYIYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLTQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4665..5990
                     /gene="pgaC"
                     /locus_tag="ACHY09_001190"
     CDS             4665..5990
                     /gene="pgaC"
                     /locus_tag="ACHY09_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFS3884191.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKTIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5992..6405
                     /gene="pgaD"
                     /locus_tag="ACHY09_001191"
     CDS             5992..6405
                     /gene="pgaD"
                     /locus_tag="ACHY09_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFS3884192.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFKKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(6455..7243)
                     /gene="phoH"
                     /locus_tag="ACHY09_001192"
     CDS             complement(6455..7243)
                     /gene="phoH"
                     /locus_tag="ACHY09_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFS3884193.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7869..9140)
                     /gene="efeB"
                     /locus_tag="ACHY09_001193"
     CDS             complement(7869..9140)
                     /gene="efeB"
                     /locus_tag="ACHY09_001193"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFS3884194.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGVISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9146..10273)
                     /gene="efeO"
                     /locus_tag="ACHY09_001194"
     CDS             complement(9146..10273)
                     /gene="efeO"
                     /locus_tag="ACHY09_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFS3884195.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKVNPELLAKVDANFIKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10331..11161)
                     /gene="efeU"
                     /locus_tag="ACHY09_001195"
     CDS             complement(10331..11161)
                     /gene="efeU"
                     /locus_tag="ACHY09_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFS3884196.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSVFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11827..13335)
                     /gene="putP"
                     /locus_tag="ACHY09_001196"
     CDS             complement(11827..13335)
                     /gene="putP"
                     /locus_tag="ACHY09_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFS3884197.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(13494..13703)
                     /locus_tag="ACHY09_001197"
     CDS             complement(13494..13703)
                     /locus_tag="ACHY09_001197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884198.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13758..17720
                     /gene="putA"
                     /locus_tag="ACHY09_001198"
     CDS             13758..17720
                     /gene="putA"
                     /locus_tag="ACHY09_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS3884199.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPPVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGDGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17760..18398)
                     /gene="rutR"
                     /locus_tag="ACHY09_001199"
     CDS             complement(17760..18398)
                     /gene="rutR"
                     /locus_tag="ACHY09_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFS3884200.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            18686..19777
                     /gene="rutA"
                     /locus_tag="ACHY09_001200"
     CDS             18686..19777
                     /gene="rutA"
                     /locus_tag="ACHY09_001200"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFS3884201.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDSLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAAHAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSDTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            19777..20469
                     /gene="rutB"
                     /locus_tag="ACHY09_001201"
     CDS             19777..20469
                     /gene="rutB"
                     /locus_tag="ACHY09_001201"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFS3884202.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            20481..20867
                     /gene="rutC"
                     /locus_tag="ACHY09_001202"
     CDS             20481..20867
                     /gene="rutC"
                     /locus_tag="ACHY09_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFS3884203.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20875..21675
                     /gene="rutD"
                     /locus_tag="ACHY09_001203"
     CDS             20875..21675
                     /gene="rutD"
                     /locus_tag="ACHY09_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFS3884204.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21685..22275
                     /gene="rutE"
                     /locus_tag="ACHY09_001204"
     CDS             21685..22275
                     /gene="rutE"
                     /locus_tag="ACHY09_001204"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFS3884205.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            22286..22780
                     /gene="rutF"
                     /locus_tag="ACHY09_001205"
     CDS             22286..22780
                     /gene="rutF"
                     /locus_tag="ACHY09_001205"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFS3884206.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22801..24129
                     /gene="rutG"
                     /locus_tag="ACHY09_001206"
     CDS             22801..24129
                     /gene="rutG"
                     /locus_tag="ACHY09_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFS3884207.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGHVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(24212..24385)
                     /gene="ymdF"
                     /locus_tag="ACHY09_001207"
     CDS             complement(24212..24385)
                     /gene="ymdF"
                     /locus_tag="ACHY09_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFS3884208.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            25347..25979
                     /locus_tag="ACHY09_001208"
     CDS             25347..25979
                     /locus_tag="ACHY09_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFS3884209.1"
                     /translation="MTSQLIPVFNGTIDNETTLLVNARDLHTFLGVGKRFASWITERI
                     AEYGFVENQDYILVSPNREIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRNIQPVQTEQQFTAEEIILLCYMQVQMENAQDISKRLYPIMKELNSSYASK
                     LYDIAFETIYMVTKNRDALLREVTRLDMSSSVIQRAMPMLKSLRARQFEF"
     gene            26027..26248
                     /locus_tag="ACHY09_001209"
     CDS             26027..26248
                     /locus_tag="ACHY09_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFS3884210.1"
                     /translation="MADIIDNAAEIEELQRNLSLQKYKSDSNAPSATHCCECGDPIDE
                     RRRLAVRGCRACASCQQDIELINKQRGVK"
     gene            26245..26529
                     /locus_tag="ACHY09_001210"
     CDS             26245..26529
                     /locus_tag="ACHY09_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFS3884211.1"
                     /translation="MNINTTITIDTALNTGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQDQRQKAIDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNEQD"
     gene            26516..26965
                     /locus_tag="ACHY09_001211"
     CDS             26516..26965
                     /locus_tag="ACHY09_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS3884212.1"
                     /translation="MSKIDYQALREAAVAIETVATPQKLLAFRMKVTPQVVLALLDER
                     ERNQQYIKSRDQENEDIALTVGKLRVELEEVKSKLNEQREYYEGVIADGSKRIAELEE
                     REILLPERSSMLHRTDFNEDYHTVMAYKVSEVIAAIRAAGIRIKGGE"
     gene            26965..27141
                     /locus_tag="ACHY09_001212"
     CDS             26965..27141
                     /locus_tag="ACHY09_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884213.1"
                     /translation="MRMAFVGLLPYPTRFWASALIAKPHVLMADNIIPAPKRRHTGIA
                     AARRAAKKRRRAKR"
     gene            27336..27554
                     /locus_tag="ACHY09_001213"
     CDS             27336..27554
                     /locus_tag="ACHY09_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFS3884214.1"
                     /translation="MPTLFRKNYPRKSRTTEFLFLILFTVLMIPISPLLLVWVIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            27556..27690
                     /locus_tag="ACHY09_001214"
                     /pseudo
     CDS             27556..27690
                     /locus_tag="ACHY09_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27915..28061
                     /locus_tag="ACHY09_001215"
                     /pseudo
     CDS             27915..28061
                     /locus_tag="ACHY09_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            28044..28487
                     /locus_tag="ACHY09_001216"
                     /pseudo
     CDS             28044..28487
                     /locus_tag="ACHY09_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(28662..29063)
                     /locus_tag="ACHY09_001217"
     CDS             complement(28662..29063)
                     /locus_tag="ACHY09_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004953517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884215.1"
                     /translation="MMFTPYRRGTILAPSGACPHLHIVCNDPVLYPELQCEGILVVNV
                     SSVPESGALYDSTCVFQGGEHRFIKHPSYVVYARSVIWRVPMLAIRIADGSIQAHDDI
                     DEEFFQPVLDGFLISKYTPYKIRRFVEKYCV"
     gene            complement(29069..29629)
                     /locus_tag="ACHY09_001218"
     CDS             complement(29069..29629)
                     /locus_tag="ACHY09_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFS3884216.1"
                     /translation="MFNEEKVAQMAAYLLKKHGGSMRFIKLMKLMYLSDRKAMELYGF
                     PISGDHYFSMNKGPVLSQTYSLVKDGSENEYSPWESWISDVADHKVEMRRTFSSVDEL
                     GELSPAEIESMDIVFGQYGHWEAFRLCDETHRICGEWRDPHGSSIPISLKDIFIALGK
                     SPEAATVMANSIYEQNNLDMLLGDLR"
     gene            complement(29753..>33208)
                     /locus_tag="ACHY09_001219"
     CDS             complement(29753..>33208)
                     /locus_tag="ACHY09_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFS3884217.1"
                     /translation="PTELNDIRLVRETIRTLGQRVREGYTPREDGAGASSQYSRSGKP
                     DPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVLRHLGAPDLPLVISRDTVR
                     KATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAVDKNGDPVVSAVHM
                     KAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKLSRDNLLHRGLQLPKGEHS
                     YRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQENIRKTG
                     GKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLDEYLYARHA
                     PERNAHIAKINPKMSDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIIDDMLARRRE
                     LIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQAMGRA
                     SRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDTMRTIA
                     ERKDQETGETIREVVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLMRAMKNMGPET
                     SNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKGLDN
                     LSALAVVKDSRSAMSAVYASLRGKNLTGKGTQWQKVWKEFVEDGGKTGWFNMGDLEGQ
                     QKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHARDAGLSRQQ
                     AASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGDGPLLERLRW
                     KNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNLVIMKSMFG
                     GKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAFSPIGSETS
                     ETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRSTPEAY
                     KAFASWLNAFSGGSQYRPGAVDVTPESLKYWIDYISGGTGRFIAKSTDAAVKSLNGID
                     IPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTAFIDENNGKL
                     SMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKIAVDRFNREYNK
                     KVGVD"
BASE COUNT         8174 a         8618 c         8305 g         8111 t
ORIGIN      
        1 tatgccgtat tcctgaagat cctcatcatt ggaatggatt ttcgggcgag aaaaggattt
       61 tatatggaca atctgctcat catttcttct tctcatcatc aacaattcac gtgtctcttc
      121 cgcgtttaat aacggattat gagatgcaaa aatatctttc ttttcagtta cctgtaatta
      181 gatatagaga gagattttgg caatacatgg agtaatacag gatgtattca agtagcagaa
      241 aaaggtgccc gaaaaccaaa tgggctttga aacttcttac tgccgcattt ttagcagcga
      301 gtcccgcggc gaagagtgct gttaataacg cctatgatgc attgattatt gaagctcgca
      361 aaggaaatac tcagccagct ttgtcatggt ttgcacaaaa atcaacactc agtaataacc
      421 aaattgctga ctggttacaa attgccttat gggccgggca agataaacag gtcattaccg
      481 tttacagccg ctaccgtcat cagcaattac cagcgcgtgg ttatgcagct gtcgccgtcg
      541 cttatcgtaa cctgcaacaa tggcaaaact cgcttacact gtggcaaaag gcgctctctc
      601 tggagccgca aaataaggat tatcaacggg gacaaatttt aaccctggca gatgcgggtc
      661 actatgatac tgcgctggtt aaacttaagc agcttaaatc tggagcgccg gacaaagcca
      721 atttactcgc agaagcctat atctataaac tgacggggcg tcatcaggat gaattacggg
      781 cgatgacaga gtcattacct gaaaatgcat ctaagcaaca atatcccaca gaatacgtgc
      841 aggcattacg taataatcaa ctagctgccg cgattgacga tgccaattta acgccagata
      901 ttcgcgctga tattcatgcc gaactgatca gactgtcgtt tatgcctacg cgcagtgaaa
      961 gtgaacgtta tgccattgcc gatcgcgccc tcgcccaata cgctgcatta gaaattctgt
     1021 ggcacgataa ctcagaccgc actgcccagt accagcgtat tcaggttgat catcttggcg
     1081 cgttattaac tcgcgatcgt tataaagacg ttatttctca ctatcagcgt ttaaaaaata
     1141 cgaggcaaat tattccgccc tgggcgcaat attgggttgc gtcggcttat ctcaaagaac
     1201 agcagccaaa aaaagcacag tcaataatga cagagctctt ttatcacaag gagaccattg
     1261 ccccggattt atccgatgaa gaacttgcgg atctctttta cagccacctg gagagtgaaa
     1321 attatcccgg cgcgctaact gtcacccaac ataccattaa tacttcgccg cctttccttc
     1381 ggttaatggg cacgcctacg agcatcccga atgatacctg gttacagggg cattcgtttc
     1441 tctcaaccgt agcaaaatat agtaatgatc ttcctcaggc tgaaatgata gccagagagc
     1501 ttgcttataa cgcaccagga aatcagggac tgcgcattga ttacgcgagt gtgttacaag
     1561 cccgcggttg gcctcgtgca gcagaaaatg aattaaaaaa agcagaagtg atcgagccac
     1621 gtaatattaa tctggaggtt gaacaagcat ggacagcatt aacgttacaa gaatggcagc
     1681 aggcagctgt cttaacgcac gatgttgtcg aacgtgaacc gcaagatccc ggcgttgtac
     1741 gtttaaaacg tgcggttgat gtacataatc ttgtagagct tcgtatagct ggttcaacag
     1801 gaattgatgc cgaaggcccg gatagtggta aacatgatgt cgacttaacc accatcgttt
     1861 attcaccacc gctgaaggat aactggcgcg gttttgctgg attcggttat gccgatggac
     1921 aatttagcga aggaaaaggg attgttcgcg actggctggc gggtgttgag tggcggtcac
     1981 gtaatatctg gctcgaggcg gagtacgctg aacgcgtttt caatcatgag cataaacccg
     2041 gcgcgcgcct gtctggctgg tatgatttta atgataactg gcgtattggt tcgcaactgg
     2101 aacgcctctc tcaccgcgtt ccattacggg caatgaaaaa tggtgttaca ggcaacagtg
     2161 ctcaggctta tgttcgctgg tatcaaaatg agcggcgtaa gtacggtgtc tcctgggctt
     2221 tcactgattt ttccgacagt aaccagcgtc aggaagtctc acttgagggt caggaacgca
     2281 tctggtcttc accatatttg attgtcgatt tcctacccaa tctgtattac gaacaaaata
     2341 cagaacacga cactccatac tacaacccta taaaaacgtt cgatattgtt ccggcatttg
     2401 aggcaagtca tttgttatgg cgaagctatg aaaatagctg ggagcaaatc ttcagcgcag
     2461 gtgttggtgc ctcctggcaa aaacattatg gcacggatgt cgtcacccaa ctcggctacg
     2521 ggcaacgtat tagctggaat gacgtgattg atgctggcgc aacgctacgc tgggaaaaac
     2581 gaccttatga cggtgacaga gaacacaact tatacgttga attcgatatg acattcagat
     2641 tttaaggata aatatgttac gtaatggaaa taaatatatc ctgatgctgg tgagtataat
     2701 tatgctcact gcgtgcatta gccagtcaag aacatcattt ataccgccac aggatcgcga
     2761 atctttactc gccgagcaac cgtggccgca taatggtttt gtggcgattt catggcataa
     2821 cgttgaagac gaagctgccg accagcgttt tatgtcagtg cggacatcag cactgcgtga
     2881 acaatttgcc tggctgcgcg agaacggtta tcaaccggtc agtattgctc aaattcgtga
     2941 agcacatcga ggaggaaaac cgctaccgga aaaagcagta gtgctgactt ttgatgacgg
     3001 ctaccagagt ttttataccc gcgtcttccc aattcttcag gccttccagt ggcctgctgt
     3061 atgggccccc gtcggcagtt gggtcgatac gccagcggat gaacaagtaa aatttggcga
     3121 tgagatggtc agtcgagaat attttgccac gtggcaacaa gtgcgagaag ttgcgcgttc
     3181 ccggctcgtt gaggtcgctt ctcatacatg gaattctcac tacggtattc aggctaatgc
     3241 caccggcagc ttattgcctg tatatgtaaa tcgtgcatat tttactgacc acgcacggta
     3301 tgaaaccgca gcagaatacc gggaaagaat tcgtctggat gctgtaaaaa tgacggaata
     3361 cctgcgtaca aaggctgagg taaatccaca cgtttttgtt tggccttatg gcgaagcgaa
     3421 tggcatagca atcgcggaat taaaaaaact cggttatgac atgttcttca cccttgaatc
     3481 aggtttggca aatgcgtcgc aattagattc cattccgcga gtattaatcg ccaataatcc
     3541 ctcattaaaa gagtttgccc agcaaattat taccgtacag gaaaaaccac tacaacgggt
     3601 aatgcatatc gatcttgatt acatttatga cgaaaaccgc cagcaaatgg atcgcaatat
     3661 tgatgtgcta attcagaggg taaaagatat gcaaatatct accgtgtatt tacaagcatt
     3721 tgctgatccc gatggtgatg ggctggttaa agaggtctgg tttccaaatc gtttgctacc
     3781 aatgaaagca gatattttta gtcgggttgc ctggcaattg cgtacccgct caggtgtaaa
     3841 catctatgcg tggatgccgg tattaagctg ggatttagat cccacattaa cgcgagtaaa
     3901 atacttacca actggggaga aaaaagcaca aattcatcct gaacaatacc gccgtctctc
     3961 ccctttcgat gacagggtca gagcacaagt tggcatgtta tatgatgatc ttgccggaca
     4021 tgctgctttt gatggcatat tgttccacga tgatgctttg ctttcagatt atgaagatgc
     4081 cagtgcagcg gctatcgcgg cttatcagca agctggcttt agcgggagtc tgagcgaaat
     4141 tcgacaaaac ccggagcaat tcaaacagtg gacccgcttt aaaagtcgtg cgttaactga
     4201 cttcacttta gaacttagtg cgcgcgtaaa agccattcgt ggtccacatg ttaaaactgc
     4261 acgaaatatt tttgcacttc cggtaataca acctgaaagt gaagcttggt ttgcacagaa
     4321 ttatgctgat ttcctaaaaa gctatgactg gaccgctatt atggctatgc cttatatgga
     4381 aggcgtcaca gaaaaatcgg ctgatcaatg gttaacacaa ttgaccaatc agatgaaaag
     4441 catcccgcag gctaaagaca aatcaatttt agaattacag gcacaaaact ggcagaaaaa
     4501 tggtcagcat caggctattt cttcacaaca actcgctcac tggatgagcc tattacaact
     4561 gaatggagtg aaaaattatg gttattatcc cgacaatttt ctgcataacc aacctgaaat
     4621 agacgttatt cgtcctgagt tttcaacagc ctggtatccg aaaaatgatt aatcgcatcg
     4681 tatcgttttt tatattatgt ctggtgttat gcatacctct atgcgtagcg tactttcact
     4741 ctggtgaact gatgatgagg ttcgttttct tctggccgtt ttttatgtcc attatgtgga
     4801 ttattggcgg cgtctatttc tgggtctatc gtgaacgcca ctggccgtgg ggagaaaacg
     4861 caccagcccc ccagttgaaa gataatccat ctatctccat tatcattccc tgttttaatg
     4921 aggagaaaaa cgttgaggaa accatacatg ccgctttagc acagcgttat gagaacattg
     4981 aagttattgc cgtaaatgat ggttcaacag acaaaacccg tgccatcctg gatcgcatgg
     5041 ctgcacaaat tccccgtctg cgggtcattc atctggcgca aaaccagggg aaaaccattg
     5101 cgcttaaaac cggagctgcc gcggcgaaaa gtgaatatct ggtgtgcatt gatggcgacg
     5161 cgttattaga ccgcgatgca gcggcatata ttgtggaacc gatgttgtac aacccacgtg
     5221 tgggtgccgt aaccggtaat cctcgtattc gcacacgttc taccctagtg ggtaaaattc
     5281 aggttggcga gtattcctca attattggtt tgatcaagcg gacccagcgt atctatggaa
     5341 atgtatttac cgtttccggt gttattgccg catttcgtcg cagcgccctg gcagaagtgg
     5401 gttactggag tgacgatatg atcaccgaag atattgatat tagctggaag ctgcagttga
     5461 atcagtggac gattttttac gagccacggg cgctgtgctg gatattaatg cctgaaacgt
     5521 taaaagggct gtggaaacag cgcctgcgct gggctcaggg cggtgcagaa gtattcctca
     5581 aaaatatgac aaggttgtgg cgaaaagaaa actttcgaat gtggccactg ttttttgaat
     5641 actgcctgac gacaatatgg gcctttacct gcctggtcgg tttcattatt tacgcagtcc
     5701 aacttgcagg tgtaccgtta aatattgaat tgacacatat cgcagcgaca catactgccg
     5761 gaatattatt gtgcacgtta tgtttactgc aatttattgt cagtctggta attgagaatc
     5821 gctatgagca caatctggct tcatcgcttt tctggattat ttggtttccg gttattttct
     5881 ggatgctaag cctggcaacg acattggtat catttacacg agtcatgttg atgcctaaaa
     5941 aacaacgcgc tcgttgggta agtcccgatc gtgggattct gagaggttaa tatgaacaat
     6001 ctaattatta cgacccgaca atcgccagta cgtttactgg ttgattatgt tgccacaacc
     6061 atcttgtgga cattatttgc tttgttcata ttcttattcg ccattgatct gctgacgggt
     6121 tattactggc aaagcgaggc cagaagccga cttcagttct attttttgct ggcagtggcg
     6181 aatgccgtcg tgttaatcgt ctgggcattg tacaataagc tgcgttttaa aaaacagcag
     6241 catcatgcag cataccaata tacgccgcaa gaatatgcag agagcttagc aatacctgat
     6301 gagctttatc agcaactaca aaaaagccac aggatgagcg tacacttcac cagtcaggga
     6361 caaataaaaa tggtggtttc agaaaaaacg ctagtccggg tataaacacc caaaacaaag
     6421 cccggttcgc ccgggctctg caccgataat acacttaact gtatgcatgt agcgtacgtt
     6481 ggcaaagtgc cgaacgtacg caatcctctt taccgaatcg gatgatcccg accatttcat
     6541 cttcttcgaa acgttccagc gcgtcactta atccggagcg cacaccgcga ggtaaatcgc
     6601 attgcgtgat atcaccgttg acgataaccg tcacgttctc cccgaggcgg gttaaaaaca
     6661 ttttcatttg cgcggcagtc acattctgcg cctcgtcaag aatgacgact gcattttcaa
     6721 aggtacgtcc acgcatatag gcgaacggcg cgatttcgac cttccctatt tctggtcgca
     6781 ggcagtactg cataaaggaa gcccctaagc gccggaccag cacgtcgtaa accgggcgaa
     6841 aatagggagc aaacttttct gcgatatctc caggtaaaaa gccaagatct tcatcggctt
     6901 gcagaactgg acgggtgacg ataatcctgt cgacatcctt atgtatcagg gcctctgccg
     6961 cttttgctgc gctgatccag gtttttccgc acccggcttc gcccgtggcg aatatcagct
     7021 gcttactctc aatagccttc agatagtgca attgcgcttc atttcgcgcg aggatgggcg
     7081 aggtatcgcg actgtcgcgg gccataccaa tggcttctac gccgctcatt tgcacaagcg
     7141 aggtgaccga ttcttcttca cgctgcttat ggctgcgcga atcccgtctc agcacacgtt
     7201 ttgcctcgcg acgagctttg atcactgctt tttgtcttcc catggagagc accttgagtt
     7261 gtttgtattc atcacacgcg ccgttggcag cgcgattatg cgcacgaaca tcagagggtt
     7321 ggcttccttg taagccatag tttgcttttg gataaaatgc cgaaaaacgg ctacgcacac
     7381 cgtttgcggc gtcggtaaca cgagtaaaga gaggaagtga gaaaaataca gagtgacgaa
     7441 gttgctaccg gaggagaaat ctccgcgagt ggtggagcct tgcttaacaa aacattgtcc
     7501 agaacaggac gttaccatcc gcgatctcca tagtgactga ctatcactgc cgggaacttc
     7561 cgctgctact taataagtac aacagatctc gcattcattg caacaatata ttcacttata
     7621 tttaaccata aaacaccatt tcagtgacat tagtttctac tggaaagatg acaaagtgat
     7681 gacagtgata aaaaagctgt gtgctttcag caggattcgc agtactggta aaagcttgcc
     7741 ggatgcggcg tgaacgcctt atccggccta ccgaacccgc accgattgta ggcatgataa
     7801 gacgcgcaag cgtcgcatca gcgcacgaac gcctggtgcg gcgtaaacgc cttatccgcc
     7861 taaaaacatt aaacccgcaa taatgcgctt ccgagataat cgttcgcgtc cttcacaccc
     7921 ggcagcgcaa agaaatagcc gccaccgata ggtttgacat attcctccag cgcctctcca
     7981 ttcaaccttt tttgtactgt caggaagccc ttttccagat cgtgttggta gcagacaaac
     8041 agtagcccca tatcgagttg cccggaattg gtgacgccca acgaataact gtagccacga
     8101 cgcagcatca gacttgactc actctctggc gtgcggggat tcgccagccg gatatggctg
     8161 tccagcgcga tcaccttccc ctccggatcg ctggcgtaat caggcacatc atgctcatgc
     8221 tgcattccca gcggcgcgcc ggtttgctta tcacgcccaa atatcgtctg ctgttctttc
     8281 agcggcgttc tgtcccaaaa ttccacccga aactgaatca accgcaccgc ctggtagcta
     8341 ccaccgattg tccacgcagg ctcctgctga tctgccgtca cccacaccac tttttgcatc
     8401 aacttcgcat tctggctatc gggattggca gtgccgtctt tgaaacccag caaattaatc
     8461 ggtgtctctt tacctttact acgcgccgcg tggtcggaaa taaccccttc ccgcttccag
     8521 cgcacgctga gcaaatccgg cgtgtgtttg atgatatcgc gcagcgcatg gatcaccgtg
     8581 tcctgggtgt tggcgcaaat ttgtagcaac acatcaccat gacataacgc cgcatccagc
     8641 gagtcgttgg ggaaacgcgt catcttctgc aactttttcg gcatctgtgg cgcaaggcca
     8701 aagcgctcat caaacaatga gtggcccacc gataacgtta tggtgagatt atcgggcgca
     8761 atgtagccgc caagaatgcc ggaatcgagt ggtggcaggc gcggatttgg cgtttctggt
     8821 gccgctccgc cctgagtcag aaaagcaaaa cgctgagtca acaagcgaaa caaccgttca
     8881 agatcggctt tatcgctggc aagcacatca aacgccacca gcatcattgc agcctgttgt
     8941 ggcgtcagga tccctgcctg atgctcacca taaaacggct gtttctcatt gcgtgcatcc
     9001 ggtgaaagcg tacccggcgc actttgcgtt ttttgtgcat gagcgaccgg acaacttccc
     9061 gccagcgcca gcgcaccaat ccctttcagt aaacgtcggc gtgacggttc attcacgccg
     9121 ttttcatctt catactgcat aacgcttagt ccagccccag cacaccgcga agttgcgcca
     9181 gatcttccgc cagcgcagta atcggtcctt tcagtgcatt ccggtcagca tcggtcagtt
     9241 tgtcgtaatt ttcaaaaccg tctttagtac ggtatttcgc cagaatggta tcgaccttta
     9301 tgaagttggc atcgactttt gccaacagtt ccggattcac tttttgtagt tgtggacgca
     9361 gcagatcgac aattttctgc gagccttcaa cgttagcctg gaaatcccac agatcggtgt
     9421 ggctgtagcg atcttcttca ccgctgattt tgctggccgc cacttcctca atcagtccgg
     9481 ctgcaccgcc gaccactttt gaaggaggga aagccagttc actgatgcgt ttttgcaaat
     9541 cgaccacatc ggtataaagc tggtcggcat acttatccat ccctttggtg gtgttatcgc
     9601 caaacaatgc tttttccaga cggtggaaac cggtgaattt cggatcggcg gctttttgct
     9661 cgtaatcatc ttcacgggcg tcaatgctgc catccagatc ggagaacagt tcagcaatcg
     9721 gttcaatgcg ctcatagtgc tggcgcgtcg gcgcatacag tgctttcgct ttttcgatat
     9781 cgcctgcttt aatagcatcg gtaaaggctt tggtgtcggt caccagctgc gtggtttccg
     9841 ccatgacata cgctttatat gcagtaattg caccaccaag acttaacagc gcatcacttt
     9901 gcgccgcatc cgccgttgcc tcacctttga cgatcaactt ccctttcggg tttgtcagca
     9961 gaccgcaggt catatcgtat tcaccaggct gtaaatttgc cgtcattttc tggctaaaac
    10021 caggggcgat attttcccgc tcttccacca ccatcacgcc tttgaggatc tcccactcca
    10081 gtgctttctg gctgtggttc tgaataatga actgtgtttt cccggcgtta accgtaatgg
    10141 tcatcggttc gcactgctta tccgtcacgg tcactttgac ctgcggcaca tcagcggcgt
    10201 tagccataaa agcagaagaa aacagcgcag ccacgctcaa ctgcaatgcg ttacggcgga
    10261 agttaatggt catgacgaga ctatcccttt aaagtatgtt gtaactaagc aagagtttgc
    10321 gtcgtatttg ttacgcggag cgagacgctg tcgcccctgc gcgtggtggc agagcaaatg
    10381 ccaccagcgc cgggatgaga taaataaacc agacggcgac ttcgctgacg ctcggcgctt
    10441 cctgatagcc aaaaatccct tccatcagcg tgccaaacac cgagtgagtt gagagcaccg
    10501 cactcatatc gaaggcgatt tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc
    10561 gaatggcacc agccgccagc cctgcggcga cgaagagaat aaacaggctg gtccatttaa
    10621 aaaatgcacc aagattgagg cgaataccgc cccagtagag caggaagccg agcaccacgg
    10681 ctgtagcaag accgagcatt gcacccagcg gcggccagat cccgacatct tgttgaaacg
    10741 ccgccagcag gaaaaagacc gactccagcc cttcccttgc aacggcaaaa aagaccatca
    10801 tcaccagcgc ccagccatga tgatttccac gctgcaatgc gctatcgact gcctgctcca
    10861 gttgtacttt gacgttgcgc gacactttgc gcatccagaa aaccatccag gtaaggatca
    10921 ccacggcgat caccgccacg ataccttcaa acagttcctg ttctttttgc ggaaattcgc
    10981 cggtggtttc gttaatgaag atacccaagc ccaggcacaa cgcagcggca agcaacacgc
    11041 caatccacat cacaccaatc catcggcctc gctgggtacg cttaagatag ctggcaatca
    11101 aactgacaat cagcgcggct tcaagtcctt cgcgcaacat aatgagaaac ggaacaaaca
    11161 tgccacccac ccttaaacgt ttttctgagt caatctatgc aaagaaaagt aaatcgcagt
    11221 gatagcgatt atcattacgg taaagaaaaa tacaagcaga atgtttataa aatgatgttg
    11281 gaatgtaaac aaagaaaggt aatgccaggg gatataaacg attattccag gtacaaagat
    11341 gggattgttt acaacgagcc atgtgttctt gccagatgcc gcgtaaatgc cttatccagc
    11401 cgacaaaact gtccagactc aatgcatcag agcttccatg taaaactgat aagcgggtgt
    11461 atcaagcaat ttcgtccatc cgcgattatc aaatctaact ttttgcccgg aacataataa
    11521 cagctcgggt gttggtggcg tcaaaatcgc tgaagcgttc cccgaaggcc ggggcagccc
    11581 acatggatgt gggctgaggg cgcgtcttac aggacgttac ctcgcgcccg acccggtagc
    11641 cgaaggggat aagctgaagg taccgcgcag cggcgatttt gttcgccaga gcccgggggt
    11701 gcagggggcg gcggcgattg gccgccccct gcgcgctccc tgcaccattc aatttatatg
    11761 gcgaggcaca ttaagggagc gcaacccgct gctgaagcca aacaaaaccg ccgcaggcta
    11821 agtcccttac ccttcctgca accgtgacgg cggagccgaa tgatagtgcg catcggcctc
    11881 ggcaaagcgt ttttgcatcg ccgctgacgg cgctttaccc agcaaactaa acactacaat
    11941 cccgatactg ccgaagataa agcccggaat aatttcgtac agacccagcc agccgaactg
    12001 tttccagacg ataaccgtca gcgcaccgat gatcatcccc gccagcgcac cgttgcgcgt
    12061 catgcgtgac cacatcaccg agaacagcac caccggacca aacgctgcgc caaagcctgc
    12121 ccacgcgtag ctcactaagc ccagcacgcg gttttccggg tttgccgcca gcgcaatcgc
    12181 caccagcgcc accaccagca ccatcactcg ccctacccac accagctctt tctggctggc
    12241 ctgtttacgc agaaacgctt tgtataaatc ttcggtaatc gcactggaac ataccagcag
    12301 ctggcaactt aacgttgaca ttaccgccgc cagaatcgcc gacagcaaaa tcccggcaat
    12361 ccacgggtta aacagaattt gcgccagttc aataaacacg cgttcagcgt tctggtttac
    12421 cgcacctgct accgccgggt gctcgttaaa gtaagcgata ccaaagaagc cgacagccac
    12481 cgcccctgcc aggcagagga tcatccaggt catactgata cgacgcgcat ggacaatgct
    12541 gtggtgagaa tctgccgcca taaaacgcgc caggatgtgc ggctgcccga agtaacccag
    12601 cccccacccc atcagcgaga taatggcgac aaaattcaac cctttgagca tatccacgtt
    12661 ttcgatgctc ttttgtttga ttacttccag cgagtcgcca aacccaccga cgctaatgat
    12721 cacgataacc ggcgtcagga tcagggcaaa aatcatcagg ctggcctgta cagtgtcagt
    12781 ccagctcacc gcgaggaaac cgccaataaa ggtgtaaagg atcgtcgccg cagcccccgc
    12841 ccacagagcc gtttcgtagc tcatgccaaa ggtactttca aacagacgcg cgcctgccac
    12901 aatgcccgaa gcgcaataaa tggtgaagaa cagcaaaata accagcgcgg agataatgcg
    12961 caaaatgcgg cttttatctt caaagcgccc ggtgaaataa tccggcaatg ttaaggcgtt
    13021 attgttgtat tcggtatgca cacgcaaccg cccggccacc agcttccagt ttatccacgc
    13081 gcctaatgtc aggccaatgg cgatccagct ttcggaaatc ccggaaagaa aaaccgcgcc
    13141 cggcaacccc attaacagcc agccgctcat atccgacgct cccgccgata atgccgtcac
    13201 gaatggccca agactacgac cgcccagaat atagtcgtcg aagtttttcg ttgatcgcca
    13261 ggcgataaac ccaatcaata tcatgccaaa gatatagaca caaaatgtca ccaacatcgg
    13321 tgtgctaata gccatctaaa gtctccaaaa aattattatc ggcaatgtcg aaacttgccg
    13381 ttatatctgc caccggaacg gggtaacaga gtttatgttt taccagggcg accgtatcct
    13441 gccggaagcg ctggttattc acaatcgatt taacacacca tttacattaa attttagtgc
    13501 tcagcgacac tatttttcat caggttgcac tctctcacat tttttgcggt tgcacctttc
    13561 aaaaatgtta actgccgcag agaaaaagtc tgagttattt ttttaatccc tgtcatatcg
    13621 atttcgttta ttaacatttc attcattttt aagcttgcta cgcaggtcac atttaacaca
    13681 gttgcacaaa gttgcaacat catggatatt tcacgataac gttaagttgc acctttcaga
    13741 acaacaggag taatggcatg ggaaccacca ccatgggggt taagctggac gacgcgacgc
    13801 gtgagcgtat taagtctgcc gcgacacgta tcgatcgcac gccacactgg ttaattaaac
    13861 aggcgatttt ttcttatctt gaacaactgg aaaacagcga tactctgccg gagctacctg
    13921 cgctgctttc tggcgctgcc aatgagagcg atgaagcacc gactccggca gaggaaccac
    13981 accagccttt cctcgacttt gccgagcaaa ttttgcccca gtcggtttcc cgcgccgcga
    14041 tcaccgctgc ctatcgccgt ccggaaaccg aagcggtttc gatgctgctg gaacaagctc
    14101 gcctgccgca gccagttgct gaacaggcgc acaaactggc gtatcaactg gccgataaac
    14161 tgcgtaatca aaaaaacgcc agtggtcgcg caggtatggt tcaggggtta ttgcaggagt
    14221 tttcgttgtc atcgcaggaa ggcgtggcgc tgatgtgtct ggcagaagcg ttgttgcgta
    14281 ttcccgacaa agccacccgt gatgcgttaa ttcgcgacaa aatcagcaac ggtaactggc
    14341 agtcacacat tggtcgtagc ccgtcgttgt ttgttaatgc cgccacctgg gggctgctgt
    14401 ttaccggcaa actggtttcc acccataacg aagccagcct ctcccgctcg ctgaaccgca
    14461 ttatcggtaa gagcggtgaa ccgctgatcc gcaaaggtgt ggatatggcg atgcgcctga
    14521 tgggcgagca gttcgtcacg ggtgaaacca ttgcagaagc gttagccaat gcacgcaagc
    14581 tggaagagaa aggtttccgt tactcctacg atatgctggg cgaagccgcg ctgaccgccg
    14641 cagatgcaca ggcgtatatg gtttcctatc agcaggcgat tcacgccatt ggtaaagcgt
    14701 ccaatggtcg tggcatctat gaaggtccgg gcatttcaat caagctgtcg gcgctgcatc
    14761 cgcgttacag ccgcgcccag tatgaccggg taatggaaga gctttaccca cgtctgaaat
    14821 cactcaccct gctggcgcgt cagtacgata ttggtattaa catcgatgcc gaagaggccg
    14881 atcgtctgga gatctccctc gatctgctgg aaaaactctg tttcgagccg gaactggcag
    14941 gctggaacgg catcggtttt gttattcagg cttatcaaaa acgctgccca ccggtgattg
    15001 attacctgat tgatctcgcc acccgcagcc gtcgccgtct gatgattcgc ctggtgaaag
    15061 gcgcgtactg ggatagtgaa atcaagcgcg cgcagatgga cggccttgaa ggttatccgg
    15121 tttatacccg caaggtgtat accgacgttt catatctcgc ctgtgcgaaa aagctgctgg
    15181 cggtgccgaa tctaatctac ccgcagttcg caacgcataa cgcccatacg ctggcggcaa
    15241 tttatcaact ggcgggtcag aactactacc cgggtcagta cgagttccag tgcctgcacg
    15301 gtatgggcga gccactgtat gagcaggtca ccgggaaagt tgccgacggc aaacttaacc
    15361 gtccgtgtcg tatttatgct cctgtcggta cccatgaaac gctgctggcg tatctggtgc
    15421 gtcgcctgct ggaaaacggt gctaacacct cgtttgttaa ccgtattgct gacacctctt
    15481 tgccactgga tgaactggtt gccgatccgg tcactgctgt agaaaaactg gcgcaacagg
    15541 aagggcaaac tggattaccg catccgaaaa ttcccctgcc gcgcgatctt tacggtgacg
    15601 ggcgcgacaa ctcggcaggg ctggatctcg ccaatgaaca ccggctggcc tcgctctctt
    15661 ctgccctgct caatagtgca ctgcaaaaat ggcaggcctt gccaatgctg gaacaatcgg
    15721 tcgcggctgg tgagatgtcg cccgttatta accctgcgga accgaaagat attgtgggct
    15781 ttgtgcgtga agccacgccg cgtgaagtag aacaggcgct ggaaagtgcg gttaataacg
    15841 cgccaatctg gtttgccact cctccggctg aacgcgcggc gattttgcat cgcgctgccg
    15901 tgctaatgga aagccagatg cagcaactga ttggtattct ggtgcgtgaa gccggaaaaa
    15961 ccttcagtaa cgccattgcc gaagtgcgcg aagcggtcga ttttctccac tactatgccg
    16021 gacaggtgcg ggatgatttc gctaacgaaa cccaccgtcc attagggcct gtggtatgta
    16081 tcagtccgtg gaacttcccg ctggctattt tcaccgggca gatcgctgcc gcactggcgg
    16141 caggtaacag cgtgctggca aaaccggcag aacaaacgcc gctgattgcc gcgcaaggga
    16201 tcgccatttt gctggaagcg ggtgtaccgc caggcgtggt gcaattgctg ccaggtcggg
    16261 gtgaaaccgt gggcgcgcaa ctgacgggtg atgatcgcgt gcgcggggtg atgtttaccg
    16321 gttcaaccga agtcgctacg ttactgcagc gcaatatcgc cagccgcctg gacgctcagg
    16381 gtcgccctat tccgctcatc gctgaaaccg gcggcatgaa cgcgatgatt gtcgattctt
    16441 cagcactgac cgaacaagtc gtaatagatg tgctggcctc ggcgttcgac agtgcaggtc
    16501 agcgttgttc ggcactgcgc gtgctgtgcc tgcaagatga gattgccgac cacacgctga
    16561 aaatgctgcg cggcgcaatg gccgaatgcc ggatgggtaa tccgggtcgc ctgaccaccg
    16621 atatcggtcc ggtgattgat agcgaagcga aagccaatat tgagcgtcat attcagacca
    16681 tgcgtagtaa aggccgtcag gtgttccagg cggtgcggga aaacagcgaa gatgcccgtg
    16741 aatggcaaag cggcaccttt gtcgctccga cgctgatcga actggatgac tttgccgaat
    16801 tacaaaaaga ggtctttggt ccggtgttgc atgtggtgcg ttacaaccgt aaccagctgc
    16861 cggcgctgat cgagcagatt aacgcttccg gttatggtct gacgcttggc gtccatacgc
    16921 gcattgatga aaccatcgcc caggtcactg gctcggcgca tgtcggtaac ctgtacgtta
    16981 accgtaatat ggtgggcgca gtggttgggg tgcaaccgtt cggcggcgaa gggttgtccg
    17041 gtaccgggcc gaaagcaggc ggtccgctct atctctaccg tctgctggcg aatcgcccgg
    17101 aaagtgcgct ggcagtgacg ctcgcgcgtc aggatgcgga gtatccggtc gatgcgcagt
    17161 tgaaagccgc attgactcag ccgctaaatg cactgcggga atgggcggca aaccgtccag
    17221 aattgcaggc gttatgtacg caatatggcg agttggcgca ggcaggaaca caacgattgt
    17281 tgccagggcc gacgggtgaa cgcaacacct ggacgctgct gccgcgtgag cgcgtgttgt
    17341 gtattgcgga tgatgaacag gatgcgctga ctcagctcgc cgccgtgctg gcggtgggta
    17401 gccaggtgct gtggccggat gatgcgctgc atcgtcagtt agtgaaggca ttgccatcgg
    17461 cagtcagcga acgtattcaa ctggcgaaag cggaaaatat aaccgctcaa ccatttgatg
    17521 cggtgatctt ccacggtgat tcggatcagc ttcgcgcatt gtgtgaagcc gttgccgcgc
    17581 gggatggtgc aattgtttcg gtgcagggtt ttgcccgtgg cgaaagcaat atccttctgg
    17641 aacggctgta tatcgagcgt tcgctgagtg tgaataccgc tgctgctggc ggtaacgcca
    17701 gtttaatgac gatcggttaa acaggtagcc ggaggatgtt acaacctcct ccggcatctt
    17761 taacgtggtc gaatcccctc aataataatc cgctgcacgt tttcaaccgt ttgattgaaa
    17821 aacacctcat cgcgcaacgt cgcgcctgtc accgcctcca cctgaggggc aaaatcggcg
    17881 taatgttgag tggaagccca aatcataaaa atcaaatgct gcgggtcaat cggcgcgagt
    17941 ttgccgcttt taacccaacc ggcaatgagc gccgatttct catcaattaa tgccttcaaa
    18001 tcgcccgtca gttcatccat taacagcggt gcacctgcca gcatctccat acagaacagg
    18061 cgcgaagcct gcggataatc gcgggaaact tccagcttca gacggatgta ttctttgatc
    18121 gccgccagcg gggcgaaatc ttctcgaaac gcttttaacg gtgccagcca gatatcgaga
    18181 atctgccgca gtacggctat atacagcgcc tcttttgacg gaaagtaata cagcagattg
    18241 gtttttgaaa cacccgccaa ctccgcgatc tgctccagcc ttgtgccgtg aaaaccgaat
    18301 tgtgaaaaag tgtccagtgc tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc
    18361 gaacgcttac ccgttgtttt cactgcgcct tgcgtcatgc gctctcccct cttgttgatt
    18421 ggcaacagga tagcaaaacc cccgcccggc ctcgaccttc tgcactctca tcgcgcacga
    18481 atgcatgttt tatgtgcaac tgttttgacc gtttagtcca ctttttttca ggcattttag
    18541 acaacttaac attaacacat taataacatt cactttttaa aaactggcat ccgctttgca
    18601 aacaagccat tacgcggctg aaaaggatat gcaggatgca gcgccccgtc tgactttcac
    18661 tttacgagac gaagagaggt tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa
    18721 cggctggctc atttcgaccc acgcgccgca gtacatgccg acatttgaac tgaataaagc
    18781 cattgtgcaa aaagcggagc actaccattt cgatttcgcc ctgtcgatga tcaaactgcg
    18841 tggctttggc ggcaaaactg agttctggga tcacaacctt gagtcgttca ccttgatggc
    18901 ggggctggca gccgtcacct cacgcattca gatatacgcg acagctgcca ccttaacgtt
    18961 acctccggca atcgtcgccc gtatggccgc aaccatcgac tccatctctg gcgggcgttt
    19021 tggcgtcaac ctcgtgactg gctggcagaa gcccgagtat gagcagatgg ggatctggcc
    19081 tggcgatgac tatttctccc gtcgttacga ctctctcacc gaatatgttc aggtgctgcg
    19141 cgacctgtgg ggctcgggga aaagcgattt taaaggcgat tttttcacta tggatgattg
    19201 tcgcgtcagt ccgcaaccga gtgtccccat gaaagtgatc tgcgccgggc aaagcgacgc
    19261 tggcatggcg ttctccgccc ggtatgccga ttttaacttc tgtttcggca aaggcgtaaa
    19321 tacacccacg gctttcgccc cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg
    19381 acgcgacgtt ggctcttatg tgttgtttat ggtgattgcc gacgaaaccg acgatgccgc
    19441 tcatgcaaaa tgggaacact acaaagcggg cgcggatgaa gaggcgttaa gctggctaac
    19501 cgaacaaagt cagaaagata cccgctccga tactgacacc aacgtccgtc agatggccga
    19561 tcccacttcg gcggtaaaca tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc
    19621 gcgcatgtta gatgaagtcg caagcgtgcc tggtgccgaa ggcgtgctgt tgaccttcga
    19681 tgattttctg tcgggaatcg aaaacttcgg cgagcgcatt caaccactga tgcagtgccg
    19741 cgcccatctc cctgcgctga ctcaggaggt ggcatgatga cgaccttaac cgcacgaccg
    19801 gaagccatta ccttcgatcc gcagcaaact gcgctgatcg tggtggatat gcaaaacgct
    19861 tatgccacgc caggcggcta cttagatctc gccgggtttg atgtctcaac cactcgcccg
    19921 gtcattgcca acattcaaac cgccgtgacc gcagcgcgag cggcagggat gttgatcatc
    19981 tggtttcaaa atggctggga tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat
    20041 tttcataaat cgaacgccct gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg
    20101 ctggcgaaag gctcctggga ttatcaactg gtggatgaac tgatgccgca gcctggcgat
    20161 attgtgctgc cgaagccgcg ctacagcggt ttcttcaata cgccgctgga cagcattttg
    20221 cgcagccgtg gaatacgcca tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa
    20281 tcgacgctac gcgacggctt ttttctggag tatttcggcg tggtgctgga agacgcaaca
    20341 caccaggcgg gaccggaatt tgcacagaaa gccgcgttgt tcaatatcga aacctttttt
    20401 ggctgggtca gcgacgtcga aacgttctgc gacgcgcttt cttccacgtc ctttgctcgt
    20461 atcgcttaag gagtttaacg atgccaaaat ccgtaattat tcctgctggc agcagcgcac
    20521 cgctggcccc cttcgttccc ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc
    20581 tggcttttga tcaacataac aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc
    20641 acgttctgga aaccatccgc aaggtcatcg agacggcggg tggcacaatg gcggatgtga
    20701 ccttcaacag catctttatt accgactgga aaaattacgc cgcgattaac gaaatctacg
    20761 ccgaattttt cccgggtgac aaaccggcgc gattctgcat tcagtgcgga ctggtaaaac
    20821 ctgacgcgct ggtggaaatc gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa
    20881 ctttcactct cacctccccc ttatgctgat gcgcccgtag tggtgttgat ttcgggtctt
    20941 ggcggtagcg gcagttactg gttaccgcaa ctggcggtgt tggttcagga gtatcaggta
    21001 gtctgttacg accagcgcgg caccggcaat aatcccgaca cgctggcaga agattacagt
    21061 atcgcccaga tggcagcgga actgcatcag gcgctggtag ccgcagggat tgagcgttac
    21121 gcggtggtcg gccatgcgct cggtgcgctg gtgggaatgc agctggcgct ggattatccc
    21181 gcgtcggtaa ctgtgctggt cagcgttaac ggctggctac gaataaacgc ccatacgcgc
    21241 cgctgttttc aggttcgcga acagttacta catagcggcg gcgcgcaggc atgggtggaa
    21301 gcgcagccgt tgttcctcta tcccgccgac tggatggcgg cccgcgcacc tcgccttgag
    21361 gcagaagacg cgctggcact ggcgcatttt caaggcaaaa ataatttact gcgtcgactt
    21421 aacgccctca aacgcgctga ctttagtcac catgcggatc gtatccgctg cccggtgcaa
    21481 atcatctgcg ccagtgatga tctgctggtg ccaacagcat gttccagtga acttcatgcc
    21541 gccctgcccg atagccagaa aatggtgatg cgctatggcg gacacgcctg caacgtgacc
    21601 gatcccgaaa cgtttaatgc tctgttactc aacgggcttg ccagcctgtt acatcaccgt
    21661 gaagccgctc tgtaaggaat tgctatgaac gaagccgtta gcccaggtgc gcttagcacc
    21721 ctgttcaccg atgcccgcac tcacaacggc tggcgggaga cacccgtcag cgatgagacg
    21781 ttacgggaga tttatgccct gatgaaatgg gggccgacat cagctaactg ttctccggca
    21841 cggatcgtgt ttatccgcac tgcagaagga aaagaacgtc tgcgcccggc gctttccagc
    21901 ggcaatctgc aaaaaaccct gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa
    21961 ttttatgaac ggttaccaca gctgtttcct cacggcgatg cccgcagttg gtttacctcc
    22021 agtccacaac ttgccgaaga aacagcgttt cgcaacagtt ccatgcaggc ggcctatctg
    22081 atcgtcgcct gccgtgcgct ggggctggat accggcccga tgtcgggctt tgaccgtcaa
    22141 tacgttgacg acgccttttt tgcgggcagc acgctgaaga gcaatctgct gattaatatc
    22201 ggctatggcg attccagcaa actttttgcg cgcctgccac gtctgtcctt tgaagaagcc
    22261 tgcgggctgt tgtaaggagc aaacaatgaa cattgtcgat caacaaacct ttcgcgatgc
    22321 gatgtcctgt atgggcgcgg cggtcaatat catcactacg gacggtccag ccgggcgcgc
    22381 cgggttcacc gccagcgccg tctgcagcgt gactgacacg ccgcccacat tactggtgtg
    22441 cctgaatcgt ggggcgtccg tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa
    22501 tacacttagc gccgggcagg agccgctttc aaaccttttt ggcggcaaaa cgcccatgga
    22561 acaccgcttt gccgccgccc gctggcagac tggtgtgacc ggatgcccgc aactggaaga
    22621 ggcactggtt tcgtttgact gccgtatcag ccaggtggtg agcgtcggca cccacgacat
    22681 tttgttttgc gccatcgaag cgattcatcg tcacgccaca ccctacgggc tggtgtggtt
    22741 cgatcgcagt tatcacgcgc tgatgcgccc tgcttgttaa cctccttaag gagacagctg
    22801 atggcaatgt tcggttttcc tcactggcag ttaaaatcga cctctacaga gagcggcgtg
    22861 gtcgcgccgg atgaacgatt gccgtttgct caaacggcag taatgggcgt tcaacacgcg
    22921 gtggcgatgt ttggcgcgac ggtgttaatg ccaattctga tggggctgga tcccaatctt
    22981 tccattttaa tgtcggggat cggcacgtta ctgtttttct ttattaccgg cgggcacgtg
    23041 cccagttatc tcggttccag cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt
    23101 aatggtcagg gaattaatcc gaatatcagc atcgcgctgg gcgggattat cgcctgtggg
    23161 ctggtttata ccgtcattgg cctggtggtg atgaaaatcg gtacgcgctg gattgaacga
    23221 ctaatgccac cggttgtgac aggtgcggta gtgatggcga ttggcctgaa cttagcgcct
    23281 atcgcagtga aaaatgtatc ggcttcggcc tttgatagct ggatggcggt aatgacggtg
    23341 ctgtgtattg ggctggtggc cgtatttacc cgtggcatga tccagcgatt attgattctc
    23401 gtaggattaa ttgtcgcctg tctgctgtat ggcgtgatga ccaacgtttt agggctggga
    23461 aaagccgtcg attttactct cgtcagccat gctgcctggt tcggtctgcc acatttttcc
    23521 actcctgctt ttaatggtca ggcaatgatg ctgattgcgc ccgtggcggt gattctggtg
    23581 gcggaaaact taggtcatct caaggcggtc gccgggatga ccggacgcaa tatggacccg
    23641 tatatggggc gggcattcgt aggcgatggg ctggcgacga tgctttctgg ctctgtcggc
    23701 ggtagcggcg taaccaccta cgccgagaat attggtgtga tggccgtgac aaaagtctac
    23761 tcgacgctgg tgtttgttgc cgctgccgtc atcgccatgc tgttgggctt ttcgccgaaa
    23821 tttggcgcgc tgatccatac tattccggcc gcggtgattg gtggtgcatc aattgttgtg
    23881 ttcgggctga ttgccgtcgc aggcgcacgg atctgggtac aaaacagtgt ggatttaagc
    23941 cagaacggta atttgattat ggtcgcagta accttagtgc tgggtgcggg tgattttgct
    24001 ctcacactgg gcggttttac gttgggaggg atcggtacag ccacctttgg cgcgatttta
    24061 ctcaatgcgt tgttaagcag aaggttggtt gacgttccac cgccagaagt tgttcatcag
    24121 gagccataaa tagaaaaccg ccggggtaac cggcggtgat gactttttga cgagtttcag
    24181 acaaaaatac attcgtcatg gattagcccg gctagttgtc gcttttaccg tgactgctct
    24241 tgcctccttt tttacctgct tcagatgcgc gctgcggatc atttttgaaa ttacccccgc
    24301 tgtgctgtcc acctttttta cctgcttctg atgctctttc gcggtcttct gcaaaattgc
    24361 cggaaccgcc tcgatggttt gccattactg acctccgtct gggtatagac atcatattgc
    24421 atagaattaa cgcggcgtat ggcggctgcc atattttcgc tacgtgggac taagcttagt
    24481 gaactgacgc gggcagaccc gcgtttagta atatcctgca acagcttctt agcgagaata
    24541 gatatcatct ccaataatta tccataagcc gctcttatga ttaaaaaaac gcgatgagat
    24601 aaaaatgtgt cattttgcga caaaattacg tgcttgtgaa agttgttata aatcaaataa
    24661 gtggttgtga aatttgcact ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat
    24721 cgctacggaa tagagataac acgaggagtg gttagaaatg gctaaagttc tggtgcttta
    24781 ttattccatg tacggacata ttgaaaccat gaagcattaa aaacacagaa ttaccgaaga
    24841 tcgtccaaga gcatatattt ttcaatctgt tacgcctcat tttgaccatg agaaaatcga
    24901 tttcacaccg ttctttatac gttctttttt gccccaaata tgccccaaac aaccacattt
    24961 tgccccaata gtgccccaaa gatttaaggg gatttaactt gatatttcag ataattaacg
    25021 caagtttgcg atgtgaattt acgttaattt tctggatgag gcatttactg ccacccgttc
    25081 atcaatattt ccgcagattg acaaatccgc cagagctatc gcatactgac cgcactgaac
    25141 ttacaagcgg tcatccgcac ccgatagctt tgcggctttt ttatgcctgc aatttggcat
    25201 agtcacatcc gtacaaaggt cgggtggaga ggcgtaatac aatacctgaa aggggaatat
    25261 gcccagagct tcttgtaagg ctcagttgac acccgatcaa ccagctacta actggttgag
    25321 ctaactaaac ttacaaggag gtcatcatga ccagtcaact catccccgta ttcaacggca
    25381 ctatcgacaa cgaaaccact cttctcgtta atgctcgcga tttacatact tttcttgggg
    25441 taggtaaacg ctttgcatcg tggattacag aacgcattgc tgaatatggt ttcgttgaaa
    25501 atcaggacta tattttggtt tccccaaatc gggaaatcaa aggtcgagga ggcgatcgcc
    25561 gtagcaaaga ctatcacctc accctcgata cagccaaaga aactgcgatg gttgagcgta
    25621 acgaaaaagg ccgccagata cgccgatact tcatcgagtg cgaaaagaaa cttcgcaaca
    25681 ttcagccagt acaaactgag cagcaattca cagccgagga aatcatcctc ctttgctaca
    25741 tgcaggtaca gatggagaat gcacaggaca tcagtaaacg cctgtacccg ataatgaagg
    25801 aactgaactc atcatacgcg agcaagctgt atgacattgc gtttgagacc atctatatgg
    25861 tgacgaagaa cagagatgcg ttactgaggg aggtaacacg tctcgacatg tcaagttccg
    25921 ttatccaacg agccatgcca atgctgaaaa gcctgcgggc aaggcaattc gaattctaaa
    25981 accaaaggag cttcggctcc ttttttcatg cctgaaggaa aggaaaatgg cagatatcat
    26041 cgacaatgcc gctgaaatcg aagaattgca gcgcaatctc tccctgcaaa aatacaaatc
    26101 cgatagtaat gccccatctg ctactcattg ttgcgagtgt ggcgatccga tagatgaacg
    26161 gcgacgcctg gctgttcgtg gatgccgagc ttgcgccagt tgccagcagg atattgaact
    26221 tatcaacaaa cagagaggtg tgaagtgaac atcaacacca cgataacgat cgatacagcc
    26281 ctgaacaccg gcctggcgct ccttggttat ttctacatca tgttctgcag cggacgatgg
    26341 ctgtcactgt tgttcatgaa aaaatggaat aaacgccgta agcaggatca acgccaaaag
    26401 gcaatagatg catttttcga agccttcggg attgacggca tggaaccagg ggatccagct
    26461 cgcgcaatta gcagaggggg cgtagtaatc cttgtatacc ggagtgaaga gaaaaatgag
    26521 caagattgac tatcaggcac tgcgtgaggc ggcagtagca attgaaacag tagcaacgcc
    26581 tcaaaaattg ctggcatttc gtatgaaagt cacacctcag gttgtgctgg cactgctgga
    26641 tgaacgggaa agaaaccagc aatacatcaa aagccgcgac caggagaacg aggatattgc
    26701 gcttacggtt gggaagctgc gagttgagct tgaggaggtg aaatcaaaac tcaacgagca
    26761 acgcgagtat tacgagggag taatcgcgga tggaagtaag cgcatagcag aactggagga
    26821 gcgggaaata ctgctcccgg aacgtagcag catgcttcat cgaacagatt ttaacgagga
    26881 ttaccacacg gtaatggcat acaaagtttc tgaagtcatc gctgcaatcc gcgccgctgg
    26941 cattcgtatc aaaggaggtg agtaatgcgt atggcatttg tcggtttatt accgtatccg
    27001 actcgttttt gggcttctgc cctaattgca aagccgcatg tcctgatggc tgacaacatc
    27061 atcccggcac caaagcgccg ccatacaggt attgcggcgg cgagacgggc agcaaagaaa
    27121 cgcaggagag caaaacgatg aaaaaccgta aagcaaaaat tctgttagct cgcagaaacg
    27181 gtgttggggt ctggcgatgg ttgaagatta gtaaccgacg agtgaggttg acggggtgtt
    27241 gcggggtgat ggggcacagt tgttgcaaaa agcccagcgc ggcgcaaaac cgctggagaa
    27301 accacggttc tcagcgaact gaatgggagt gatatatgcc tacactattc agaaaaaatt
    27361 atccgagaaa gagtagaaca acagaattcc tgtttctcat tctgtttacc gtgttgatga
    27421 taccgatatc cccgctatta ctggtctggg taatcggaaa aataattgag ccagttattg
    27481 aattgtataa cgacgtggta tgggcgtcgt tcaacacact gcacaataaa attaatccgt
    27541 ataaggaaag ctgatatgac cacttttacc aaagagcgac tgctgacaat caagcagtgg
    27601 cgcgaaacat acggacctgg tagcaacgta gtgattccag cagaagaagc ggaagagctg
    27661 gcacggattg cactggcatc gctggaagca gagcctgtgg cgtcatgcat tattgaagat
    27721 gggggcatgt gtgttgacgg gttcggtgag tatgttggtc actcgctgcc tgatggaacg
    27781 caccagctat acgccgcccc gccagtgcct gataagttgc cgcgtgaata cataagaggt
    27841 tggcctcttg cgcatagtga ttatgcagaa ggatggaacg attgccgtgc tgcaatgctt
    27901 aatggagata aatcatgatt gatcgaacca aactggagca catcctcgaa tatgccaggc
    27961 agcagaagca tactggtcag cattgtaaaa ttccactagg ggatatggtt gaaatcatgg
    28021 agattgccat gcgcaaggct ggcaactctc cgataactcc gggtcgtttg cctggcggtt
    28081 tcaccattga ggaggcgaag gagttacatg aagacctggt gcgcagccac ataagcaagg
    28141 ctttaagtgg cgaaaagatg aagaagaagg atctcgatgc tgatttgcgc tggatccatg
    28201 gggttatagt ccaggcagcc tggtttgtaa aagcatcact ggagcagaat gcgctgtctg
    28261 gcaactctcc ggcaactccg gctggctgga tacgctgtag tgagagaatg ccgccagttg
    28321 gtgttgatgt acaactgttt gtgtccagca cgggtgaaca gtttacaggt tttaacctgg
    28381 ataatacggg tgacttccag tatgcgcagt gtttcggtac gcccatcgta tgtgaagcca
    28441 cacactggat gccgctacca gaaccgccga aagaggtgcg ccaatgagct ggcctgatgc
    28501 atttgcaatt gtaggggctg ccatttcgat cgcactgatt gtttttgcga tttgccgctg
    28561 ggggtaaaat agttcacctg catggagatt tgatgccggg gaattgtggg ggagcattca
    28621 acctctcccc ttcccggtta aagagttgac ttgtctgctt atcaaacaca gtatttttca
    28681 acaaaacgcc gaattttata cggtgtatat ttggatataa ggaaaccatc caacacaggc
    28741 tgaaaaaact cctcatcgat atcatcgtgg gcctgaatac ttccatcagc aattctgatc
    28801 gccagcattg gcacccgcca tattacagac ctggcataaa caacgtagct gggatgttta
    28861 atgaatctgt gctctcctcc ctgaaaaaca cacgtcgaat catacagagc accactttct
    28921 ggcaccgagg agacattgac aacaaggata ccttcgcact gaagttccgg atataacacc
    28981 gggtcattac acacaatatg caagtgggga caggctcctg aaggtgccag tattgttccc
    29041 cggcgataag gggtgaacat catcccggtt acctcagatc gcctaataac atatcaagat
    29101 tattttgctc ataaatgcta ttcgccatta ccgttgctgc ttctggcgac tttccaagag
    29161 caataaatat atccttaagg ctgattggaa ttgatgaacc atgcggatcg cgccactcac
    29221 cgcaaattct gtgggtttca tcacaaagcc tgaacgcttc ccaatgcccg tattgaccaa
    29281 aaactatgtc cattgactct atttctgcgg gcgataactc ccccagctca tcaacagaag
    29341 aaaatgtacg ccgcatttca accttatgat ccgctacatc agatatccat gattcccacg
    29401 gactgtactc gttttcgctt ccatccttaa ccaaagaata tgtctgcgat aaaacggggc
    29461 ctttgttcat agagaaataa tgatcaccac taatgggaaa accatacaac tccattgctt
    29521 tgcggtcaga gagatacatc agcttcatca gcttaatgaa acgcatagat ccgccatgct
    29581 ttttcagcaa atacgctgcc atttgcgcaa ctttttcttc attaaacata aatcggctct
    29641 ctgtctcaca tgaaatgctc tttctgacta tatgttaata gctccggtta ttacaggtca
    29701 atgtgtgttt atttcttaat cagccccgca cttccgtacg gggccatttc tgttaatcca
    29761 ctcccacttt tttgttgtac tcgcggttaa accgatccac ggcaattttc atatcccgct
    29821 ctaccgattt caccatcgcc gactgttgcg ccagactgag agtactgtcg gcgtaaatgg
    29881 catcacgctg tttacgcaaa tccttcagtc tcttccgggt atcctgcata agcccgttca
    29941 tcgacaattt tccgttgttc tcgtcaatga aggccgttct ttctgcaccg gtcagactct
    30001 tcagctctgc gtgatactgc gcgacctccg tcatccggtc gtacatcttc tgctggtcag
    30061 catacggcat cacctcacct gaaattttcc ccaggaaagg cacctgctgc tccggtatat
    30121 caataccatt cagcgatttc accgccgcat ccgtcgattt ggcaataaaa cgccctgtac
    30181 caccagagat atagtcaatc cagtatttca gtgattccgg cgtgacatcc accgcgccgg
    30241 gacggtactg gctgccacct gagaacgcat tcagccagga tgcaaacgcc ttgtacgctt
    30301 ctggcgttga acgtcttccc agttggctgt ccggttttgg tgtaccaaac ggcatgttct
    30361 cctggtaaat ctgcgccccc atgaagtttt cattcatggc aaggttcgca aacggacgca
    30421 gaatggtcgg cgctgcattt ttcagcaatg ccccggacag tgtttccgac gtttcactac
    30481 caatcgggct gaatgcacca agcacaccac caacaacatt accggcagca cgggacgcag
    30541 tcaggtcacc cgccgccaca ccttcagcgg tatgcccgag caggaagaaa acgttgtacc
    30601 cgtaaggcag aggaatactc cagtactctc cggccttgcc cccgaacatc gatttcatga
    30661 tgacgaggtt acgctctttc acatgagacg gtactttgtc ataccagtta accccgtcat
    30721 catcctcacc cgccacgctg cggttaagcg agccaagcag ataacccgct cccacagcgg
    30781 caagcgcgat tttctgcggt acattcagat tcttccagcg aaggcgctcc agtaacggcc
    30841 cgtcgccatt aagatgtccg agcgttctca ccaggtttgc ggtcccctga atactggcgt
    30901 tggcgaacat gtacagcgag ttcatcagcg ccccctgctc accacgacgg ttaaagttca
    30961 ccgtcatgtt tttggcaaga gacgccgcct gctggcgtga caaaccggca tcacgggcgt
    31021 gtttataagc tgacagacgc agtgcgtttt caaccgcccc gttggcatcc tccacaaggt
    31081 tcaggaacga attccatgca ccgatactct ggcctttcca tccccccttc gccagcgata
    31141 caaggcgatc catttccttc tgctggcctt caaggtcacc catgttaaac cagccggttt
    31201 tacctccgtc ctcaacaaac tctttccaca ccttctgcca ctgtgtacct ttgcccgtga
    31261 ggtttttgcc acgcagactg gcgtatacgg ctgacatagc agaacggctg tctttcacca
    31321 cagccagtgc ggataagtta tccagccctt tcagtttgcc gtcgctcctt ccctgctccg
    31381 ccttcagatt catcaccgct gtctgcacgt cacggatgaa gttactgacc aggaattccg
    31441 ggttatacga cgtgttcacc gttgccagga agcggttaac tttccccagc gtacggatta
    31501 ctgcgttact ggtttccggt cccatattct tcatcgcacg catcaggcgc ggatcatgga
    31561 gtttgatgta gtacgttttg ccgttctttt tggtggtgaa gtaccggtct gccatcattg
    31621 ccatcggtac agggcgttcg acaacttcgc gaatggtttc accagtttcc tggtccttgc
    31681 gctctgcaat cgtccgcatg gtatccggtc tgtcatcggt gaatacctgc cagtaatcct
    31741 tgtcgggatt atcctgcacc agtttcagga aggcgttacc cacttcgttt ttgcgatggc
    31801 ggatcagcga ttcgctcagg tcctgtatcg cctgagtgga cggagactgt gcccgggatg
    31861 cacgccccat ggcctgcctg ctttcacgtc cgccgatggt gaagccctta cctgtacggg
    31921 gcagtgacac cacaccgtca acatcctgac ctttcagggg aacgtagtaa cggtaggcgt
    31981 tctgccaggc atccaccaca ccgttctctt caagtccggc ctcacggata agctcacggc
    32041 gacgggccag catatcgtca ataatccctg ccagacggtc atactgtgcc tgtttgccac
    32101 tgttacgtac acgctgcatg atttccgccg cttccgcgtt ggtcatcccc gaaccgccgt
    32161 ccgacatttt cgggttgatt ttcgcgatat gcgcgttacg ttccggcgcg tgacgggcgt
    32221 agaggtactc atccagatcg gcctgcgcaa ttttgtagtc cgccagtaat ttagccagtg
    32281 gctgaacgta gcgctccttc atcacgttca ggtcgttttc cgccttcccg tggaagagtt
    32341 cttccgccat ataagcgttg ttactgtcgt ccactttgcc gccagtttta cggatattct
    32401 cctgaacagc tttcagcacc tggaatttat cctgcatctg gcgcacaaaa cgcgatgcaa
    32461 ttgtctcttc cggtgtcaga ctgctggtac gggagtaata cggccccttg cgaatatctt
    32521 caggatagag tattttatcc gcagagccgc gataagaatg ttcccctttg ggcaattgga
    32581 gccctctgtg cagaaggtta tcgcggctta atttttctct ccggtacaac gttaacccgg
    32641 tcatttccat actcttcagt ttttttccat tttctgtacc gtaaacagaa gctaccttgt
    32701 tgatttccag acgcgatcgg acagccttca tgtgtaccgc tgacaccacc ggatcaccat
    32761 ttttatccac ggcatcaagc agcatcacaa ccgcatttct ttctgtcgct gagcggtaaa
    32821 ttgcatccgg atcgtgcatc agttccggta gtctctcgat aacatccatc ggcaccacat
    32881 gtttcacacc attggtggcc ttccgcacag tatcgcggga aataaccagc ggcaaatccg
    32941 gtgcaccaag gtgacgcaat accggcggcg tacgcccgat gtttaccgtt aaatctgtgg
    33001 tacgcagaga tttcatcatt ctggcaaggt catcgcgata acgttcaccc tcaccttccg
    33061 gcactttgaa cggatcaggt ttaccactac gggagtactg agacgatgca cccgcgccat
    33121 cctcacgcgg cgtgtaacct tcccgcacac gctggcctaa cgtacggatg gtctcgcgaa
    33181 caagtctgat atcgttcagt tccgtcgg
//
LOCUS       DAZUCC010000029        33081 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZUCC010000029 DAZUCC010000000
VERSION     DAZUCC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.029"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(32..397)
                     /gene="yhaH"
                     /locus_tag="ACHY09_001220"
     CDS             complement(32..397)
                     /gene="yhaH"
                     /locus_tag="ACHY09_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS3884218.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(688..1674)
                     /gene="yqjG"
                     /locus_tag="ACHY09_001221"
     CDS             complement(688..1674)
                     /gene="yqjG"
                     /locus_tag="ACHY09_001221"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFS3884219.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFNHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1744..2136)
                     /gene="yqjF"
                     /locus_tag="ACHY09_001222"
     CDS             complement(1744..2136)
                     /gene="yqjF"
                     /locus_tag="ACHY09_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFS3884220.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2323..2622)
                     /gene="yqjK"
                     /locus_tag="ACHY09_001223"
     CDS             complement(2323..2622)
                     /gene="yqjK"
                     /locus_tag="ACHY09_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFS3884221.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2612..3016)
                     /gene="yqjE"
                     /locus_tag="ACHY09_001224"
     CDS             complement(2612..3016)
                     /gene="yqjE"
                     /locus_tag="ACHY09_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS3884222.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3019..3324)
                     /gene="yqjD"
                     /locus_tag="ACHY09_001225"
     CDS             complement(3019..3324)
                     /gene="yqjD"
                     /locus_tag="ACHY09_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFS3884223.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3362..3730)
                     /gene="yqjC"
                     /locus_tag="ACHY09_001226"
     CDS             complement(3362..3730)
                     /gene="yqjC"
                     /locus_tag="ACHY09_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFS3884224.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3877..4260)
                     /gene="mzrA"
                     /locus_tag="ACHY09_001227"
     CDS             complement(3877..4260)
                     /gene="mzrA"
                     /locus_tag="ACHY09_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFS3884225.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4264..4926)
                     /gene="yqjA"
                     /locus_tag="ACHY09_001228"
     CDS             complement(4264..4926)
                     /gene="yqjA"
                     /locus_tag="ACHY09_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFS3884226.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4948..5031)
                     /locus_tag="ACHY09_001229"
     CDS             complement(4948..5031)
                     /locus_tag="ACHY09_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884227.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            complement(5271..6047)
                     /gene="exuR"
                     /locus_tag="ACHY09_001230"
     CDS             complement(5271..6047)
                     /gene="exuR"
                     /locus_tag="ACHY09_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFS3884228.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQATDNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6177..7475)
                     /gene="exuT"
                     /locus_tag="ACHY09_001231"
     CDS             complement(6177..7475)
                     /gene="exuT"
                     /locus_tag="ACHY09_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFS3884229.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            7958..9370
                     /gene="uxaC"
                     /locus_tag="ACHY09_001232"
     CDS             7958..9370
                     /gene="uxaC"
                     /locus_tag="ACHY09_001232"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS3884230.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9385..10872
                     /gene="uxaA"
                     /locus_tag="ACHY09_001233"
     CDS             9385..10872
                     /gene="uxaA"
                     /locus_tag="ACHY09_001233"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFS3884231.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10955..11506
                     /gene="ygjV"
                     /locus_tag="ACHY09_001234"
     CDS             10955..11506
                     /gene="ygjV"
                     /locus_tag="ACHY09_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFS3884232.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11511..12755)
                     /gene="sstT"
                     /locus_tag="ACHY09_001235"
     CDS             complement(11511..12755)
                     /gene="sstT"
                     /locus_tag="ACHY09_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS3884233.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLINDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13371..14336)
                     /gene="alx"
                     /locus_tag="ACHY09_001236"
     CDS             complement(13371..14336)
                     /gene="alx"
                     /locus_tag="ACHY09_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFS3884234.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(14619..15605)
                     /gene="ygjR"
                     /locus_tag="ACHY09_001237"
     CDS             complement(14619..15605)
                     /gene="ygjR"
                     /locus_tag="ACHY09_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS3884235.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKNVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15684..16367)
                     /gene="ygjQ"
                     /locus_tag="ACHY09_001238"
     CDS             complement(15684..16367)
                     /gene="ygjQ"
                     /locus_tag="ACHY09_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFS3884236.1"
                     /translation="MQARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTSKQL
                     TWSDVSAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVGAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAQK"
     gene            complement(16444..16947)
                     /gene="ygjP"
                     /locus_tag="ACHY09_001239"
     CDS             complement(16444..16947)
                     /gene="ygjP"
                     /locus_tag="ACHY09_001239"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFS3884237.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17032..18168
                     /gene="rlmG"
                     /locus_tag="ACHY09_001240"
     CDS             17032..18168
                     /gene="rlmG"
                     /locus_tag="ACHY09_001240"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFS3884238.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18359..18442
                     /locus_tag="ACHY09_001241"
                     /pseudo
     CDS             18359..18442
                     /locus_tag="ACHY09_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            18439..18855
                     /gene="higA"
                     /locus_tag="ACHY09_001242"
     CDS             18439..18855
                     /gene="higA"
                     /locus_tag="ACHY09_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFS3884239.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18900..20918)
                     /gene="fadH"
                     /locus_tag="ACHY09_001243"
     CDS             complement(18900..20918)
                     /gene="fadH"
                     /locus_tag="ACHY09_001243"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFS3884240.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQLPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVGLAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KSHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQQLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(21089..23440)
                     /gene="ygjK"
                     /locus_tag="ACHY09_001244"
     CDS             complement(21089..23440)
                     /gene="ygjK"
                     /locus_tag="ACHY09_001244"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS3884241.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSATLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNKRNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKAETLSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(23457..24527)
                     /gene="ygjJ"
                     /locus_tag="ACHY09_001245"
     CDS             complement(23457..24527)
                     /gene="ygjJ"
                     /locus_tag="ACHY09_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFS3884242.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDTKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVSGSES
                     GMMVSAMYEALKGIYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            complement(24661..26094)
                     /gene="ygjI"
                     /locus_tag="ACHY09_001246"
     CDS             complement(24661..26094)
                     /gene="ygjI"
                     /locus_tag="ACHY09_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3884243.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(26157..26606)
                     /gene="ebgC"
                     /locus_tag="ACHY09_001247"
     CDS             complement(26157..26606)
                     /gene="ebgC"
                     /locus_tag="ACHY09_001247"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFS3884244.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVIYDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(26603..29695)
                     /gene="ebgA"
                     /locus_tag="ACHY09_001248"
     CDS             complement(26603..29695)
                     /gene="ebgA"
                     /locus_tag="ACHY09_001248"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFS3884245.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENNLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(29879..30862)
                     /gene="ebgR"
                     /locus_tag="ACHY09_001249"
     CDS             complement(29879..30862)
                     /gene="ebgR"
                     /locus_tag="ACHY09_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFS3884246.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            31081..31413
                     /gene="ygjH"
                     /locus_tag="ACHY09_001250"
     CDS             31081..31413
                     /gene="ygjH"
                     /locus_tag="ACHY09_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFS3884247.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            complement(31455..32834)
                     /gene="ygjG"
                     /locus_tag="ACHY09_001251"
     CDS             complement(31455..32834)
                     /gene="ygjG"
                     /locus_tag="ACHY09_001251"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFS3884248.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCEQVIKAARKALA
                     AMRVSVEEA"
BASE COUNT         8057 a         8709 c         8554 g         7761 t
ORIGIN      
        1 atgagtcaga catccggcga gtagatttgt tttaaggttc cagttttgga tcaggtccaa
       61 agcggttttc gcctggcgta cccgcctggc agttaaagac gatgataata agccagccga
      121 taaacgggat taagaacagc agtgcccacc acgccgagcg gtcggtgtcg tgcaggcggc
      181 ggaactgaac tgcccaccac ggtaaaaaca ccagaatacc gtagattgtc gtcaggatgc
      241 cttcgccacc ggcacgttgc cagcctaaca ttttatccag cagccccagc acgaacgtaa
      301 agatgatgtt gaccagaata aacatccagt actctttgcg ccgcgcacga ccccggaaac
      361 cgacataatt ttttagtact ttcagatacc agtccatttt tgcctcaatt ttgctcaatc
      421 acttgttttt aaagcagcaa attaagaata gacgtgaatg aaatggcagt aaatattcgt
      481 tcaaggaatt aataaagtgc cgtaaagcgc gctaagactg atgacaagcg caaaatcgcc
      541 agatgcgcta cgcttatctg gcctacgcgg cattgtaaca tattgaatat aaacgttttg
      601 taggttggag aagacgttca cgccgcatcc gacataaata acacgcactt agctaataat
      661 cagaggcacc tcgtaaggtg ccttttttta accgaagcga acatctcgtc catgcggttc
      721 atcgagatcc tgccacggac caattgaaat aatccccgta gggttgatgg tcttatggct
      781 gcggaagtaa tgattacgga tgtgattgaa attgacagtt tcggcgatcc ccggcatctg
      841 gtagatatcg cgcagaaaac cgtacagatt caggtaatcg ctgatgcggt gcttatcaca
      901 cttgaagtgg gtcacataca ccggatcaaa acggaccagc gtggtccaca ggcgaatatc
      961 ggcttcggtt agctgattgc cggtcaggta acgatgctgg cctaaaatct gctccagtcg
     1021 cgccagcgat tcaaacactt tcgccaccgc ctcgtcgtaa gcttgctggc tggtggcaaa
     1081 accagcttta tacacgccgt tgttaacggt gtcataaatc cagccgttaa gttcgtcaat
     1141 tttcgtttgc agggctggtg ggtagtaatc acccgctttc gcgcccagcg catcaaacgc
     1201 ggtattgaac atgcggatga tttccgctga ttcgttgctg acgatggtgt ggttcttttt
     1261 gtcccacagt acgggaacag taactcgtcc gctgtagtgt ggatcggcgt ggagataaag
     1321 ctgatacaaa aattcatgtt gatagagtgt atcgccggtt gcacccggaa aactgtcatc
     1381 aaaggtccag ccgttttcca gcatcagcgg gttcactaca gaaacggaaa taaacggttc
     1441 cagtcctttg agtttgcgca tgatcagcgt gcggtgcgcc cacgggcagg cgagtgaaac
     1501 atagagatga taacgatctt tctctgcgat aaaaccgcct gtgccagtgg ggcctggcgc
     1561 gccatcggca gtgagccagt tacgaaatgc ggaagctgaa cgttgaaatt taccgccggt
     1621 agatttggta tcgtaccagg tgtcatgcca gacgccgtca atcagttgac ccatatttct
     1681 ctcctccaat agcaaaaacg aggagcttcc tcctcgtttt ttatcgttaa gtatagcgcg
     1741 tgcttaccac tttttattca gcaggcggtc gatgctatac gcgcccggac cggtaattgc
     1801 cagcagcagg aatccgccag aaattgtcag gtttttcatg aacatcagcg agttgacgcc
     1861 ttcagcaaag ttgctgtgaa ataaaaatgc cgtcagcagc gtaaagcccg cagtaaacag
     1921 ggctgtggtg cgagtcagga aaccgaacag gattgccaga ccaccaccaa actcaagcag
     1981 aatcaccagc ggcagcataa aacccgggac gcccatcgct tccatatatt gttgggtacc
     2041 cgcgtaacca gtaatttttc cccagcctgc ggtaataaac agaatcggca ttaaaatgcg
     2101 cgctaccagt acaccaacat cttctaattt tttcatcata ctctccactg cgccgctcag
     2161 gctgctgatt gtttttttgt gcaagggcgc gctattagcg tcgttgctgt cgatggagag
     2221 aatcataaac gtggcgaagc gtgattgtta gcaaggaaaa ctgtcaaata ttttcaaaaa
     2281 atttgaggga taaggccgga atttctccgg ccagagagga agttaaccgc gaagctgttg
     2341 ttgcttgagg gttgttttca ccagacgcca ggcgctccat acaccaaaac cgcgtctggc
     2401 ccagcggacc agcatattag gatggcgaat cgtccagata gccatcacgc tactgccaac
     2461 cagcgcccag gagcgcagac tcaacagcat attccagcga cgatcgtaag cgcctgttgc
     2521 ctccagccat tcacgacgac tggcggaaag atccagccgt tgttgctgga tctggctaag
     2581 cagttgcgcc ttacgtcgtt cacgttcgac tttactgctc acgggactcc tcctcgagca
     2641 gctgccgatc gtttgccagc tcatggcgcg tgtggcgcag caacgtagac ttacgcgatt
     2701 tacgtaacgt ccagatgccg ccaatcagtg ccagtagcag caacaccacg gtggtggcaa
     2761 tcatcgcatt caggcgatat tgcggatcaa ccgcccagat aattagcacc atcaggctca
     2821 taagaccaaa tgcagcgaaa agcatcgtca ggcccagcat cagtaaaagt tgaaagagat
     2881 tcgctttttc ctcttccagc tccaccaccg ccagccgcag acgtgtctcc accatttcaa
     2941 ccatgatgga aacaattcgc tgcccgatgc ccagaacgct tttaccgggc ccttgcgcgt
     3001 gatgagtgtc cgccataatt aacgacgcga cagcagaaca ccgagcacta caccgattgc
     3061 tgcgccaatg cccacgcccg tccacggatt ttcacgcaca tactcatcgg cacgcgccgc
     3121 cgcgacacgg gtttgtttgg caatggcatc accggtttca cccaggcgat aacggctctg
     3181 tttcagtgcc tgctccgctt tgctacgaat cttactcaac tcttctttcg acttctcgcc
     3241 agatgagcta agcacctctt ccagcgtatc ggaaagggat ttcaactcag cacgcagatg
     3301 ttccgtagtg tgttctttcg acatagtttt ctccaggtga gtaagtagtg actattgtga
     3361 gttagtagtc gcgcgcttcc agctttttca gctcttcctg cgcttctgcc agtttccgtt
     3421 cgcgtttggc aatcttatct gcatcgcctt tttgcttcgc ctcggctaaa tcttgctggc
     3481 gttccgccac ctcatctttc tgctttgcga ttttcttctg atgatcggca cgcagctggc
     3541 tatctgaaca gttggcccgg acttcactca gggctttatt cagaccgtca atacgattct
     3601 ggttttggtg tttttcggca tagctgatct ccttaaggat attttgctcc ttttcctgac
     3661 acagggtagt ggcataacta ccggcactaa gagcaaagag agaaacagct aaagcgatgc
     3721 ggtatttcat tcttgaacct tccattgtga ttcggaccta ttacaaagga acgattgtcc
     3781 agacaacgaa gcggtcgtgt taaccaccgc ctcattaatc atagacagtt acggtgaaat
     3841 caccaaagtg agtgatctgt ttcggataac aggaagttat ccgaagcgat gagagttatc
     3901 ccgcaaccgg gtcagccact gcatagcctg actgttattg tcctgctgcg caatgatgta
     3961 gccatggggc aatgttctgt cgaggaccgc ttttgcggcg gcgctctggt cgctggagtc
     4021 aaaggtaatt aacagagtgt cgtttttggg ggtgatactt ttgaaaggaa tgccgtgagc
     4081 gtcaaggtga tgccagattg aaaaaccgtc tggcatcgtt gtcccttgat gaacggcacg
     4141 aatcgccagc gtagactctt gctggcgaac cgcggaccag gccagcaaca gtgtgccgac
     4201 cagaagtaaa acagcgccgg accaggctag ctggcgaagc gacatgcgag gtatttgcat
     4261 cccttacccc cgatttccat atttcttttt ccataacacg accagagaac ctgccaggcc
     4321 aaacaccagc agcaccaccg ggagcagcat caggcatgac atcagctggt cctcgtactt
     4381 taaaaatacc ggcgttttgc cgagcatgta acccagagtt gtcaggatca atacccacaa
     4441 caaaccactc atccagttga agaactgaaa gcgcgcgtta ttcagccctg ataacccggc
     4501 aatcgtcggc agcagtgttc tgacaaacgc aataaagcga ccaattaaca gcgccgataa
     4561 accgtgttta tgaaaaagat ggtgtgcgcg ttgatgataa tgcgcgggta aatgagatag
     4621 ccagttttgt acggtgcggg tattgcccag ccatcgcccc tgaatatagc tgacccagca
     4681 gccgaggctg gcggcaacgg tcagcagcag aatcgtttgc ggatagccca tcgcgccttt
     4741 cgcaatcaat acaccgacca acaccagtaa gctgtcgccc ggtaaaaagg ctgccggaag
     4801 caagccgttt tcaaggaaca aaatgacaaa caagacaaaa tacaacatgc caatcatcga
     4861 cggattggcc agggtttcaa aatcctgcgc ccacagggct tgcagcaatt gggtcaaaag
     4921 ttccattcat tattcctggt gaatcggtta acgccacgct ggttttaaat gatgcactac
     4981 cgcaatgtta ttgctgtctt ggtatgttct gcgggtttga tacatcaaca cagcctgaac
     5041 tattccctgt taaatcgtga gataagaaag cattcaaaag cataaattca aattgaatct
     5101 aattgtaaca aaggtcaacg ttgttcgtta tcaaaattac atgtccgtgg agtctgattt
     5161 tgctgaatgc aggggaggga ggcgagagga tttacacagg ctgacacttt atacttttcg
     5221 cttaccgacg agacaaacag gcgcactgta gcgcctgtcg gacaggaatt tcatttactg
     5281 ccgctggtgg ctgtatcgag atgcaccacc gggttttcgg cgaacagata gcggtcggca
     5341 ttgaactcga aatcgtcgct ggtttcgtta aataacatga tcttggtgtt ttccaggtgc
     5401 tgccacattg ccagcttagc agcatgagga tctttgcgaa tcagcgcctt gaggatttga
     5461 tcgtggtcat cacaccagtt atcgacggta cgggcatcaa tgtgttcgtg cagttttttc
     5521 cagtacgggt tatgactacg ctgggtccac attttttcca cgatagccgc cagggcggag
     5581 ttctgcgtcg ccagggctac ctgaatgtgg aactgcaaat cccactcgga atcacggaag
     5641 cattgttcgc cgcgcgcctg ctcctggatg gccatcagtt tcatgatgtc ctgtttcgtc
     5701 acctgagttg ccgcgaactc ggcaatatta ctttcgatga gctggcgagc ctggagcaac
     5761 tcaaacggac cgtaattggc gaattccata ttattgtcag tcgcctgctg atggcgcggc
     5821 tggttggaaa ccacatgaat acctgaacct ttacgcactt cgacatagcc ttcaacttcc
     5881 agcatgatga tggcttcacg aaccacggtg cggctgacgt tcttttcatc ggcaataaag
     5941 cgttcagcag gcagtttatc acccaccaga tagacgccct gttcgatgcg ctctttcaga
     6001 tcagcggcaa gttgttgata caaacggcgt ggttcagtga tttccatatg cgctccaggc
     6061 atgatacggc agactatatt tgttatacca cttttgcgag tttagctcta ctcacagtga
     6121 tgaaaaagcc gcctgagaag gcggctttga agggaaacta aaacttacac gaagccttaa
     6181 tgttgcggtg cgggatcgtt atgtgattcc tgcgccacct caatcgccgg tttgttctgc
     6241 aacacggtcc agataaccag cgcacctaac aggtcgaaca ctgccagaac tgcgaacagc
     6301 gggctgaagc cgatggtgtc agccagtgca ccgactacca gtgcaaacag cgtacttgcc
     6361 agccatgcgg acatcccggt taaaccgttc gccgtcgcca cttcgttacg accgaacacg
     6421 tcagacgaga gcgtaatcag tgcgccagac agtgcctggt gagcaaaacc accgatacac
     6481 agcagcataa tcgcgacata cgggttggtg aacagaccga tcatacccgg gccaatcatc
     6541 agcaccgcac ccagcgttac gaccatctta cgggaaacga tcaggttcac accaaaccaa
     6601 cgctggaaca gtggtggcag gtaaccaccg aggatacaac cgaggtcagc aaacagcatc
     6661 ggcatccagg caaacatcgc gatctctttc aggttaaagc cgtaaacttt aaacatgaac
     6721 agcgggatcc acgcgttaaa agtaccccat gccggttctg ccaggaagcg cggcagcgcg
     6781 ataccccaga actgacggtt acgcaggatc tgaccaacgg acattttctt cgctgtgtca
     6841 acctggtgct gggcttcctg accattaata atgtagtcgc gttcttcatc ggtcagatgt
     6901 ttctggtcgc gcggatgttt atagaaaatc agccatgcca tcgcccagat aaagctcaac
     6961 gcaccggaga tgataaatgc catctgccag ctgtgcatca cgattgccca taccaccagc
     7021 ggcggcgcaa tcatcgcacc aatcgaagaa cctacgttaa agtaacctac cgcgatggaa
     7081 cgctctttcg ccgggaacca ttcggagctg gctttcagac ccgccggaat catcgcggct
     7141 tccgcggcac cgaccgcacc acgagcaaca gccaggccgc cccagctacc tgccagcgcg
     7201 gttgcaccac agaacacggc ccacagcaca gcaaacattg cataaccgat tttcgtaccc
     7261 agcacatcca gtacatagcc cgctaccggt tgcatgaccg tataagcagc agaataggct
     7321 gcgatgatat aggaatactg ttgggtggag atgtttaact cttccatcag agttggcgca
     7381 gctgccgcca cagtgttacg cgtcaggtaa ccaagcacgg tgccgagcgt caccagtgcg
     7441 atcatatacc aacgtaaccc tttaatttta cgcatgtaaa acctcatcgt tttgttatat
     7501 ccgcaggaag cgcggccatc atcccgccag aggctgaaga tgttagttac gaccgggtaa
     7561 ccgaaaaaaa accggcacgc cccgaacgtt gccatgaaag tttcgtgcta attcggcttc
     7621 cgtaccggtt aacccgattc atctaacaaa tgaatgaatc ggcaatttgc attccgtcga
     7681 cttatgattt gcgacggcag aaagataact tgtcatacaa ctttaaaagg tgagagccat
     7741 cacaaatgtg cgaatatttg tagggacatt acctgacgac ggcgaggcca gtactggcgc
     7801 ggcctgcagc gagatttacc actttgagag taattttttt aactacgttt attgatctaa
     7861 ctcacgaaaa tatcttcgga ctctggaaat tggtgtgata actttgtcag catcgcacca
     7921 taagcaagct agctcactcg ttgagaggaa gacgaaaatg actccgttta tgactgaaga
     7981 tttcctgtta gataccgaat ttgcccgccg tctgtatcac gactacgcaa aagaccagcc
     8041 gattttcgat taccattgcc atttgccgcc gcagcagatt gcggaagact atcgttttaa
     8101 aaacctgtat gacatctggt tgaagggcga tcactacaaa tggcgcgcta tgcgtaccaa
     8161 cggtgtggcc gagcgtctgt gcaccggtga tgcgtctgac cgtgaaaaat ttgacgcctg
     8221 ggcggcgact gttccgcaca ctattggcaa cccgttatac cactggacgc acctcgaact
     8281 gcgtcgtccg tttggtatca ctggcaaatt gctttctccg tcaactgccg atgaaatctg
     8341 gaacgaatgt aacgaactgc tggcgcagga taacttctct gcgcgcggca tcatgcagca
     8401 gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc gattctctgg agcatcacgc
     8461 agagatcgcc aaagacggct ctttcaccat caaagtgctg ccgagctggc gtccggacaa
     8521 agcctttaac atcgaacagg cgacctttaa cgactacatg gcgaagctgg gtgaagtctc
     8581 tgataccgac atccgccgct ttgctgatct gcaaactgcc ctgaccaaac gtctggatca
     8641 cttcgccgct cacggctgta aagtgtctga ccacgcgctg gatgtggtga tgtttgctga
     8701 agcgaacgaa gcggaactgg acagcattct ggcgcgccgt ctggcaggcg aaaccctgag
     8761 cgagcacgaa gtggcacagt tcaaaactgc ggtgctggtg ttccttggtg ccgaatacgc
     8821 acgtcgcggc tgggtacagc agtaccacat cggcgcgctg cgtaataaca acctgcgtca
     8881 gttcaaactg ctggggccgg atgtaggctt tgactccatc aatgaccgtc cgatggcaga
     8941 agagctgtct aagctgctga gcaagcagaa cgaagaaaac ctgctgccga aaaccattct
     9001 ctactgcctg aacccgcgcg ataacgaagt gctgggcacc atgatcggta acttccaggg
     9061 cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg tggtttaacg atcagaaaga
     9121 cggtatggaa cgtcagatga cccaactggc gcagctcggt ctgttgagcc gctttgtcgg
     9181 tatgctgact gacagccgta gcttcctgtc atacacccgt cacgaatact tccgccgcat
     9241 tctgtgccag atgatcggtc gctgggtgga agcgggcgaa gcaccggcgg acatcaacct
     9301 gctgggcgag atggtgaaaa atatttgctt taacaatgcg cgtgactact tcgccattga
     9361 actgaactaa ggtctggggt tgatatgcaa tacatcaaga tccatgcgct ggataacgtc
     9421 gcggtcgctt tagcagattt ggctgaaggc acagaagtca gtgtcgataa ccagactgtt
     9481 acgctgcgcc aggatgttgc tcgtggacat aaatttgcgt taacggatat cgcaaaaggg
     9541 gccaacgtca tcaaatatgg cctgccgatt ggttatgcat tggcggatat tgcggcgggg
     9601 gaacacgtcc acgcccacaa tacgcgcacg aatctgagcg atctggatca gtatcgctat
     9661 caacctgatt ttcaggatct gcctgcgcaa gcggcagatc gtgaagtgca gatctatcgt
     9721 cgcgctaacg gcgatgtcgg ggtgcgtaat gagctgtgga tcctgccaac cgtgggctgt
     9781 gtcaacggca tcgcgcggca gatccaaaat cgtttcctga aagagaccaa caacgccgaa
     9841 ggtactgacg gcgtgttcct cttcagccac acctacggct gctcacagct gggcgacgat
     9901 cacatcaata cccgcaccat gctgcaaaac atggtgcgcc acccgaacgc aggcgcagtg
     9961 ctggtgattg gtctgggctg tgaaaacaac caggttgccg cattccgtga aacgctgggc
    10021 gatatcgatc ctgaacgcgt tcatttcatg atctgccaac agcaggatga tgagatcgaa
    10081 gccgggatcg cgcatttgca tcagctgtat aacgtgatgc gcaacgataa acgcgagcca
    10141 ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg gttctgacgg tctttctggt
    10201 attactgcta acccgatgct ggggcgtttc tctgactacg tgattgctaa cggtggtact
    10261 accgtactga ccgaagtgcc ggagatgttt ggcgcagagc agttgctgat ggaccattgc
    10321 cgcgacgaag caacgtttga aaaactggtc accatggtca acgacttcaa gcagtacttt
    10381 attgcccatg accagccgat ctacgaaaac ccatcaccgg ggaacaaagc aggcggtatc
    10441 accacgctgg aagataaatc gcttggctgt acccagaaag cgggttccag tgtcgtggtt
    10501 gacgtgctgc gttacggcga gcgtctgaaa acgccggggc tgaacttgtt aagtgcgccg
    10561 ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg gctgccatat ggtgctgttc
    10621 agtactggtc gtggcacgcc gtatggtgga tttgtgccga cggtgaaaat cgccaccaac
    10681 agtgaactgg cggcgaagaa aaaacactgg atcgactttg acgcgggtca gctgatccac
    10741 ggtaaagcga tgccgcagtt gctggaagaa ttcatcgata ccatcgttga gtttgccaac
    10801 ggtaagcaaa cctgtaacga gcgtaacgac ttccgcgaac tggcgatctt caaaagcggc
    10861 gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg gcgtttcata atgtgaagcg
    10921 ccgtttgctt ttccagacct gctaaggatt tatcatgacc gcgtattggc tggcccaggg
    10981 cgtgggtgtc atcgcctttc tgattggtat cacaacattt ttcaaccgtg acgaacgccg
    11041 ctttaaaaag cagctttcgg tctatagcgc cgttattggc gtacattttt ttcttctggg
    11101 cacctatccc gccggtgcca gtgccatcct taatgccatt cgtacgctga ttactttacg
    11161 cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg actggcggaa ttggcctcgc
    11221 gaagttccat catcctgtcg aactactgcc ggtaatcggc acgattgtca gtacctgggc
    11281 gctgttccgc tgtaaagggc tgaccatgcg ctgcgtaatg tggttttcaa cgtgttgctg
    11341 ggtgattcac aacttctggg cggggtcgat aggcggcacg atgattgagg ggagttttct
    11401 gctgatgaat ggtctgaata tcattcgttt ctggcggatg cagaaaaggg gaattgatcc
    11461 gtttaaagta gagaaaaccc ctcccgccat agacgaaagg ggttaaacaa ttaattacgc
    11521 agggcgctat ttgccagacg atcgtcttcc gcctggcaag ctgccgcagt gaacagcacg
    11581 tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca atacgccgat gataaagccg
    11641 acggcaacca cctgcatggc gatatcgttc gaaataccga acatattaca ggccagtggg
    11701 atcagcagca gagacccccc cgccacgccg gatgcgccac aggcacacag agaagccact
    11761 acgctcaaca gcagcgctgt gggcagatcg accggaatac ccagcgtatt aaccgcagcc
    11821 agcgtcaaca cggtaatggt gattgctgcg cccgccatat tgatggtggc tcccagcgga
    11881 atagaaacag aataggtatc gcgatccaga ttcagctttt cacacagcgc catattcacc
    11941 ggaatgttcg ctgcagagct gcgggtgaag aaggcataca caccgctttc acgcaggcac
    12001 agcagcacca gcgggaacgg gttacgacga attttccacc acaccagcaa tgggttaacc
    12061 accagcgcca ccagcagcat acagccaacc agcacgacca gcagttgcgc gtagccccac
    12121 agcgtggaga aaccggtggt tgccagggta gaagaaacca gaccaaaaat accgatcggt
    12181 gcgaagcgaa tcaccagttt caccataaag gtgacggcat tcgacatatc gttgatcagg
    12241 tttttggtgg tctcgttacc gtgacgcagt gcgaaaccga ggccaattgc ccacaccagg
    12301 atcccgatat agttaccttt tagcagcgcg tcaatggggt tggaaaccat gctcattacc
    12361 agcccgcgca ttacttctac aatgcctgac ggcggcgaaa tatcacccgc gctactggac
    12421 aagtgcaggg tagaagggaa ggcaaaactg aagattactg cggccagagc agcagagaag
    12481 gtgcccagca gatagaggaa caaaataggg cggatattgg ttttctgccc gtgctggtgg
    12541 ttagcaatag atgccattac cagcatcaac accaggatgg gggcaacggc tttcagtgcg
    12601 ccgacgaaca aagtacctaa cagaccaaca gcttccgccg cgggttttga gatccatgcc
    12661 agaagaatcc ccagaacaag gccgaccagg atttgtttta ccaggctgcc atgagccaga
    12721 cgccggaata gccccggtga acgttgcgta gtcatttttc gatcctttca ttgtgttgtc
    12781 gcacatcctt ggtgcgttct gtcgacgcat ctttcaggat gtaacaaagt gtttgcaagg
    12841 tcgagtataa ggaaagttac agcttcggga agcgaaaaat gctggatttt aaagagaggt
    12901 catattttgc aacttaatgg ttacatttat ttgacataaa cagagggaag gactggctac
    12961 taaggctgat tagataacat atgggcaaaa ctatttggaa tatgagaacg gttgcgctcc
    13021 tttaatgtgc tatgccatat aaagccactg gcgcagggag cgcgcagggg gcggccaatc
    13081 gccgccgccc cctgcacccc caggctctgg cgaacaaaat cgccgctgcg cggttccctc
    13141 agcttatccc ttgcggctac cgggtcgggc gcaagggaac gtccctgtaa gatgcgccct
    13201 cgccgcgcat ccatgcgcgt cgccccggcc tccagggaac gcttcggcga ttttgacgcc
    13261 accaacaccc gtgcggtctt tttgtaaaga gctataagcc gattacataa ttaatagctc
    13321 acctttgtct ggaatgagtt atctctagta atggcttaat atacagcgaa tcattctccc
    13381 cgctgcttat catgccgata attcacccag gcgttgataa taaacgtcat caccagaatg
    13441 ccaaacacca cgcccagcga gacggcgatc ggaatatggt agaagtcgac aatcagcatc
    13501 ttgataccga taaacaccag aatcaccgcc agaccatatt tgagcatcga gaaacgctct
    13561 gccacacctg ccagcaggaa atacatcgca cgcaggccga ggatcgcaaa caggtttgag
    13621 gtcagcacaa taaacggatc ggtggttacg gcgaagatag ccggaatact atccacggcg
    13681 aaaatcacgt cgctcaactc caccagaatc agcaccagca tcagcggcgt ggcatacaac
    13741 aaaccatttt tacgcacaaa gaaatgctcg ttgtcgatgg tgtcggtcat gcgcaaatga
    13801 ccgcgtagcc agcgcaccag cggcttgtcg ccaatgcctg attcatcttc atgggcgagc
    13861 gccatcttca cgccggtaaa cagcaggaag gcaccgaaga tatacaggat ccagtcgaac
    13921 tgtgaaatca gccagctgcc agtgaagatc atgatggtac gcagaacaat cgccccgagc
    13981 acgccataca ccagcacgcg gcgttgtaat gccgccggaa cagagaaata gctgaacaac
    14041 atcagccaga caaagacgtt atcgaccgcc agcgatttct caatcagata acccgtgaga
    14101 aaggccagtg cctgtggatc ggcaacggcg cgaccttcgg tttgcaccag ataccaccag
    14161 aaagcggcgt taaacagtaa cgacagcgtc acccagacca gcgaccaggc cgcagcctgt
    14221 ttcatggtca tggcatgtgc cccacgacgc ccctgcaaca acaggtcgat agccagcata
    14281 atggtgacaa caacagcgaa tccgccccat agcaacggcg tgccgacagt attcatagaa
    14341 gttccttaca cataaaaaaa cggccaacgt cggaagacgt cagccgctgc tttttatgca
    14401 tagacctcgc cttccggcaa ggtctcactt acaacaaaaa aggaatacgt ctgcgtattc
    14461 ctttcgggtt gcccggtgac cggatgtggt ttttcacaca tcgtaatgac gatcgaccga
    14521 caatgaagtt actccccttt gcaggtaaca aagtatgaca gaccattcag tcagtcaatg
    14581 gtctgttaca taccttttac actgttttac tttggcaatt atagttttac gctatctgcc
    14641 ggaaaaatca ccccagtctg gcggcggatc tcggtcagca gtttagcggt gatgcgactg
    14701 accgccagcc ctggatgatc caccagatgc tcatccacca gttcagcaaa cagctctgct
    14761 tcgtagagca tggtattaat atgctgcggt tgggtcagat cctgcatctg gctgccacgc
    14821 ggcacaaaac acactttctg gcattcagac agtttttcaa tcaccagcga tcccgcttcg
    14881 ccctgaatct cgcttgccag gacagaatcg ctcacttttg agtgctgcaa cgtaacgctg
    14941 aaatcaccgt aatccatcac caccacgccg tgggcatcca caccgcttgc cagcaaactg
    15001 gcggttgcct gcacgttttt cggttcaccg aataatgcca ccgccgacgc cagacagtaa
    15061 aagccgatat ccataattga accgttagag aatgctggat taaaggtgtt gggattctca
    15121 ccatccaggt aacgttgata ccgcgaggaa tactggcaat agttgaaaaa gactttacgc
    15181 agtttgccga ctttcggcag cgcctggcgc aacagatgaa agttcggcag gtaggccgtt
    15241 ttaaatgcct caaacagcac cacctgattt tcccgcgcac aggcaatggc ggcatccact
    15301 tccgccagat tcgatgccag cggcttctcg caaatcacat gaattttgtg actaaggaaa
    15361 agttgtgtct gggaaaagtg cagggcattc gggctggcaa tatacaccgc gtcaatggca
    15421 tcgctttccg ccatcgcttc cagcgacgta aacagatgct cgacagaaaa atcattggca
    15481 aagtgctggg cctgttcaag gctgcgggaa tatacggcgg ttaacttgta tttaccgctc
    15541 tcatgggcgg cctcgacgaa ctggcgagtg atccagttcg taccaatcac agcgaaacgt
    15601 atcataacgc gtggatactc cataaaggga ttctgccgtc aatgtagcac gcctttttca
    15661 tcttcgacgt gcgcagatac gcattatttc tgcgccggac agccatgctc actaaacggc
    15721 ccaatcatga ccgatggccc gagatatttc ggctggcgat ggaggatttt tgcgtcaatc
    15781 accgcgccga cgcgagcgag tttctcccgc agttgagtat aaaaaccgtg cttcatattg
    15841 aggtctggcg cgttaaaacc gatagcgtcg atgccgtact gttttgccag ccagatggcg
    15901 cgttggttat gaaattcttg cgaaataatg gtgatatggt tttccccaaa cacctttttg
    15961 gcacgcacca ccgaatcaag cgttgagaat ccggcatagt cgcagaagat cactttcgcc
    16021 ggtacgcctt tagcgatcaa agcctgctgc atacctgagg cttcatcgta atttttacgc
    16081 ccgttatcgc cacttaccag tagccatttc acttttccgg cgtggtacaa cgctgctgcg
    16141 gtatcaatac gtcgggtaaa gtagcgatta cccggcctcg cccccagcaa taaacccacg
    16201 ttacgcgccg gaactgcgct gacatcgctc caggtcagct gcttgctggt gttgaccata
    16261 acgcgatcgg caataaaaat ggtcgctcct gctataagca acaataaaca gccaatttta
    16321 agagtccggc gagaaaaaca aatacggaga agaaggcggg cctgcatggt cgttatcctt
    16381 aacgaaaggg gggaggtcga ttttaggcgc tataggaaga aaagataacg cctgacatca
    16441 ttttcagatt ttgtcctgcc caagcgacaa ctgcgttagc cacagccggg tgtcgaactc
    16501 aagctggtga tactgcggct ccatatggca gcacaattga tagaacgctt tgttatgctc
    16561 tttctccttc aggtgcgcca actcgtgcac cacgatcatg cgcaaaaacg gttccggcgc
    16621 gttgcgaaac acggtagcga cgcggatctc cgcctttgct ttcagcttgc cgccctgcac
    16681 acgcgaaacg gcagtatgta atccgagcgc gtttttcagc acgtggatct tattgtcata
    16741 catcacttta gtgatcggcg gggcattgcg cagaaactga tttttaagat cctgagtata
    16801 ttgccagagg gctttatcgg tggcgtagtc gtgtgttccc ggatagcgtt ttgccagcac
    16861 atctcccaga cgctgttcgt taatcagcgt tcgcacctgg gaaagtagct gctcgggata
    16921 accctgaaga taagtaagat tgctcataac cgcccacgaa aaagagaaaa agggtatact
    16981 cacgcaccct tttcaggggc aacgtcgaaa ttttatcatt caggagggcc gatgagccac
    17041 ttagacaacg gtttccgttc actgacacta caacgttttc cggcgacgga tgacgttaac
    17101 ccactacagg cgtgggaagc ggcggatgaa tatttgctgc aacagttgga cgacacagaa
    17161 atccgcggcc cggtgttgat cctgaatgat gcctttggtg cgttaagttg tgcgctggcg
    17221 gaacataagc catacagcat tggcgactca tacatcagcg aactggcgac gcgcgagaat
    17281 ttacgcctca acgggattga tgagtcgagc gtgaagtttc tcgacagcac cgccgactac
    17341 ccgcaacagc cgggcgtggt actgatcaaa gtaccgaaaa cactggcgtt gctggaacaa
    17401 caactgcgtg cgctgcgcaa agtggtcacg ccggatacac gtattattgc cggtgccaaa
    17461 gcccgtgaca tccacacttc cacgctggaa ctgttcgaaa aagtgctcgg tccgaccacc
    17521 accacgctgg catggaagaa agcgcgcctg attaactgca ccttcaacga accgccgttg
    17581 gccgatgcgc cgcagaccgt tagctggaag ctggaaggca ctgactggac tatccacaac
    17641 catgcgaatg ttttctcccg cactgggctg gatattggcg cgcgcttctt tatgcaacat
    17701 ctgccagaga atctcgaagg tgagattgtc gatctcggtt gcggtaatgg cgttattggc
    17761 ctgacattgc tcgataaaaa cccgcaggcg aaagtggtgt ttgtcgatga atcaccgatg
    17821 gcggttgctt ccagccgttt gaacgttgaa accaacatgc ccgaggcgtt ggatcgctgc
    17881 gagtttatga tcaacaacgc gctctccggc gtggagcctt tccgttttaa tgctgtgctc
    17941 tgcaacccgc cgtttcatca gcaacatgcg ctgaccgata acgtcgcctg ggagatgttc
    18001 caccatgccc gccgctgcct gaaaatcaac ggcgagctgt atatcgttgc caaccgtcac
    18061 ctggattact tccataaact gaagaaaatt ttcggcaact gcaccaccat cgccacgaat
    18121 aataaatttg tggtgctgaa agcagtgaag ctggggcgtc gtcgataaga ttgggagtta
    18181 cctgccggat gcggcgtaaa cgccttatcc ggcctacgtt cagcacatta cccccaggtc
    18241 ggataagacg cggcaagcgt cgcatccggc aatgtctgcc cagttctgga atcgcgcttc
    18301 cataaaacaa aaaatccctg tcaccatcct ctacattctc ttgtttagcg tttttctacg
    18361 tttatgctac atacgtgaag ttatgacgca taaagaatac gatttcttta ccgccgttca
    18421 tcgtactaag gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta
    18481 actgctgtgg caccttttct ggcgggtatt cagaacgagg aacaatacgc ccaggcgctg
    18541 gaactggtcg atcatctgct gctcaacgat cctgaaaatc ccttgctgga tctggtgtgt
    18601 gccaaaataa ccgcgtggga agaatcagcg cccgaatttg ctgaatttaa tgccatggct
    18661 caagccatgc ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc
    18721 ctttccgatc ttccggaaat tggcagtaaa tccatggtgt cacgcgtttt gagcgggaag
    18781 aggaaattaa cgctggaaca cgctaaaaaa ctggcaacgc gattcggcat ttctcccgcc
    18841 ttgtttattg attaacgcga cgtgccggat gcgtcgcatc cggcgcttca aacaacgatt
    18901 taaatctcca gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc aagctccata
    18961 gctacatcgc agccgccaat taaatgcaca gttttcccgc tatcaatcag ctgttgcgcc
    19021 agcgcgcggt ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc
    19081 gtttcgccgt tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct
    19141 gggatcattt tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg
    19201 cctaacccct gtcccggttt gctggctttg cgctggagca tcacaatctg ccgtgggcta
    19261 cgcgggatct gcattccctg cgggcttaag ccaccagcct gttgcaggct gctgtcgatc
    19321 ccccactcat tacagaactc ggcaatgttc tggctggtgg actcacccgg ctgacttaaa
    19381 aacatcgccg tatcaaaacc aatcccgcca caaccgatga ttgctacctt tttgcccaca
    19441 ggcgctttgt cgcgcagtac gtcgagataa ctcaacacct tcggatgatc gattccgtcg
    19501 ataggtggaa tgcgcggtac gatcccgcta gcgaggatcg tttcatcgaa cgactgtaac
    19561 tgatccgccg tcacggtgtg attgagtttc agcgtcacgc ctgttacttc gatcatccgg
    19621 cggtaatagc gcagcgtttc gtaaaactcc tctttgccag ggatctgttt ggcgatatta
    19681 aactgaccgc cgatctcact gtgagcatca aacaacgtta cctgatgacc acgtgccgcc
    19741 gcgttaatgg caaacgccag cccagccgga ccagcgccga ccaccgccag attttttttc
    19801 tgcacggcgg gaaggatcgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac
    19861 gaggtgactt tgccaacgaa gatctgatcg aggcaggctt gattgcagcc aatacaggtg
    19921 ttgatctcat cggctcgccc cgattgcgct tttgacagca gttccgcatc agcaagaaac
    19981 ggtcgcgcca tcgataccat atcggcatca ccgcgcgaga gaatgtcgtc ggcaacctgc
    20041 ggatcgttaa tccggttggt ggtcaccagc ggcagcgaga cgtggctttt cagtttgcgc
    20101 gtgacccagc taaaggcacc gcgcggcacg ggcgtggcaa tggtcggaat acgtgcttca
    20161 tgccagccaa tgccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagc
    20221 cctaccgttt ctgcaaaagt accgccgcct tctaccaggt cgagcatcga cagacggtag
    20281 ataataatga agtcattgcc gacgcgttcg cgcactgctc gcaccacttc aactgcaaag
    20341 cgcatccggt tgcggtaatc gccgccccac tggtcgctac gctggttggt gcgcagggtc
    20401 agaaactcgt tgatcaaata cccttcggaa cccatcactt ctacgccgtc gtatccggcc
    20461 tcccgcgcca gttgcgcgca gcgggcgaaa ttgtcgatca gttgcaggat ctcttcatgg
    20521 cttaactcat ggggcacgaa acggttgatg ggggcctgca atgcggaggg agcgaccaga
    20581 tgcggttggt agctgtagcg cccggtatgc aaaatttgta aggcaatttt gccgccttcc
    20641 tgatgtacgg cttcggtaat ggtgcgatgg tgtgggagct ggctggcgtc gttgagcatt
    20701 gcgccgcctt ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcaac
    20761 gccacgccat gacgggcgcg ttccgcgtaa aacgctgcca gccgttcagc gccgtccggg
    20821 tattcctcca gtccggtgtg cattgaaccc atcagtactc ggttttttaa cgtggtgaag
    20881 cctaaatcca gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat
    20941 aattgttatg tggtcggatg agttctaatt tagccgtcgc tggagaaaag ggaaaagggg
    21001 agtgcggggt ttgtgatggg attcaaaaag aatacgcccg ttcgtcatgc cggacggcgg
    21061 taatacagtc gtccggcatc gggaaaaatt actgcttacg gaaaaaatcg ttatacaaca
    21121 tatacaaatg cgcggcactc caggagaaat ttggtgcgcc ttgctgtgcg ccagtcagcg
    21181 ggttgtaatt ctcctgaatt gggccatcgg cggttaatcc tttggcgtgc cggaagaacg
    21241 tatccgccag cttcagggca tcatcgcgat aaccgtaacg ctccatccct ttcagaccaa
    21301 accagaactg atccacccac acgcgcccgc gccagtaaat atctgcgcca aaggccggat
    21361 tggttaacgc cgccgttccc agcggtacaa aggtgttgaa ctctttagga tcgagcatca
    21421 ccttcaccac cgcgtcggcg ttggcctgcg ttgccgcacc gttaaacagc ggcgaccagc
    21481 cttccggccc tttgccgcgc tcgacaatcg gtttgcccgc gcagccgttc gccagtggtt
    21541 tatcttcaat gcgcacgtca tagtagaact gggtagtcgg gtcgaacata caggtgttga
    21601 tgtagtccgc gagctgttgt gctaactgac gatagcgttt ggcctcttcc ggcttaccga
    21661 gaatcgtcgc catctccgcc agataatggt tatcgctgta catatagctg gcctgatcca
    21721 ccgactcctg caatagcgag tagcccagca acgttccgtc ctgactgcgg ttttcggcga
    21781 atttcaccgt ccagtcgcta cgtttgccgc cgttagcgac atatttatcc agctgttctt
    21841 tgtcgataaa cccaaagacg gcggcatcat cacgccccga ttcccacgac gccgcaacct
    21901 gtgccgggat ttccagactg tcgtactggc ctttctctac cacgcgggcg tagttgttca
    21961 gcccagacag cgtctctgct ttgttgcctt tcttcaccgt aaacagcatc tcgccgctct
    22021 cagtgttgtg ggctttgtcg cgggtcgcgc catattccgg cacgccgttg ccgttgtgat
    22081 cgcggttacg tagccaccag tcgtgatagg ccaccagttt cgggtacatc tctgccagcc
    22141 aggttttatc ctgggtgacg ttatacactt ccattactga ccaggcggca aggctgggtt
    22201 tggtattacg tttgttccag ttgccgccat cgccgccacg ctcggggcta aggttccatg
    22261 ctatcaggtc ggggacaaag cccacatcct gcggacgcac gcggtcgcct ggctggatct
    22321 gccaggagaa gaccgcgcgg atattctctt tggcgatatc cggattgaaa tgcgccatcg
    22381 caaatgcctg cttccaggta tcccacggcc aggtctgatt gccggagaac cagcgcccgg
    22441 tcaccgacgg cgtgacggtg ttatatttca ccgccccccc aggcgagcgc cagttaccgt
    22501 tgagcgtttc gatcgctttc accgcgacgc gcgtctgttc cggcgtcgca tccggattgg
    22561 ttaacccttt cttcagatac tcttcccagc gttgctgcga ggcggtgaga taaaacgccg
    22621 gacgcgccag aatatcgcgg atctgcattt gctctttgct aacttcctga gcggtcagca
    22681 ggtgggagta ggtggtatag agcgtggccg aaccgttgat atgcgcctta ctggtaaagc
    22741 gattaccgtt gatttcagtc tgcactggca gggatttatg cacctgatat tctgattcac
    22801 cggaggtcag cagatcccag gtggcgcgca ctttgccaaa ggtgactttc aggccatcac
    22861 gggtggcgct gattttgcgc tgatagtcag ggtattcgcc agcaatggtt ttatcggaaa
    22921 gcggtttccc ttctttcgct tccagttttt ccagcagctc gccgtcccac accagatcca
    22981 gcggtttatt gctgatgatt ttagtttcca gtagtgacgt gcgcggcgtg gcgaagcgca
    23041 gagtcatttc gacctgcaca tcttttgctg tcagtttttg caccagcgcg ccgggaatac
    23101 tgtatgcctc cagcgtgaag tcgactttct tgccgtcttg ccagacggtc agacgatcga
    23161 aattgctggc cataaagttg atgtattctt ccgtcagcag cgcaacgcct ggaaaaccgc
    23221 ccatggtgtt cgggccgtct ggcaacagat gaccatgcca ggctccgaga tcgaaaaacg
    23281 gattaaaacg ctggtgatcg tcgtaatcgt aatccttcat gtactgcggc gcgccagtac
    23341 ggttaatcac gtttttataa ttgtcggcgt tagcggcatg ggcggaaaaa cttatcagca
    23401 gagcgcaggt tactggcgtt aaaatagttt tgattttcat gaaattacag tccttattac
    23461 cagtagaaat attgcatcag gaagagttgg tcgctgggat cgtgagaggc atttttcatg
    23521 tagaagcggc tatcaaaagc cgttgcgaaa cgtccgccgc cgtattcgta ccagatacca
    23581 aactgcatga aagaggtggt ttcatcgtca gcgtggtcag ggcgatgttt ggcgtaggtg
    23641 taagcggtgg agagataaat gccttttaac gcttcataca tggcgctcac catcatcccg
    23701 ctttcacttc ccgatacgtc cagcccgtcg gcgcgtccgg tatggtgcca ggcgcgagcg
    23761 gcaaagttag gggtaagcag gcccgtcaga tagagttggc ctgtgccgtc ggtgatttcc
    23821 agaccgttca tccaggtgat gccttcggca aggtcatact ggatgtaacc gttcatcatt
    23881 gccggatagg tgtatttgtc gtcgtagcga tcgtacttgc caaaatgtag ccaggctttg
    23941 ctttcgtcat gatgtccggc aggtgtggcg gtaacgctat aacgaaagtt gccgcttaag
    24001 ttctgcacct tcagcgcggt cataatgtcg cgggtattgg gaatgacgta gcccagatcg
    24061 ggcgtgaaat cgccccacca ttgcaggtca tccagcgagg aatcctgacg cagactgagc
    24121 atgatttcgg tgccatcatt ggtacgataa ccgccataga aacggttaat cccaccttcg
    24181 aaaccgcccc agctatggtc aggcacccag gcattaccgt cgtggtcggc ctgcacggtc
    24241 cagccttcgc cgtaaatcag gccgaaccag gatccgtact tcatctccag gccaccttca
    24301 atataggtgc cgtcgctgta tttatgttta ttctcaccct ctaaatccat atacccacca
    24361 acccctaatt cgccgtaaaa acgaaatgaa ggagaagatg tcgaggtcat tgcgggggtt
    24421 tttgtgtcgt catgttctgc cggacgtgct tcatcagcgg tcacagaaaa ggcgtagcaa
    24481 aacggcagcg caagtaatgc tctgcatggt gcagtgatta atttcattgt gttaatccat
    24541 gagctaaaga aaaagtacag ccttttatcg ttgtaattat aaaaatataa aggcagaaac
    24601 tattttccct ctccccatcc aggaagaggg aaaatgcctg ctcatttttt attattctgc
    24661 ttaatcaaca ttgctggctg gcgtcgcttc aatatggcgc tcttcagccg ttaatccctt
    24721 tatatattta ctgtatttcc accacgcaaa gccgaggaag ataacaatac cgccgacgtt
    24781 ataaaaaatg atggtcagaa tattcgcgcc agtcgggaat gtcgaagcga caaaccctac
    24841 ggcaaatatc gcaatcagca ttgaaacaac gataataccg gtgcggcggg agcccatacg
    24901 gaaatcgcgt ggcaggtgat ctaatttggc gcgtaaattc aggtaagcca gcatgatgaa
    24961 taacggcgga agcatggacg ctgcggcggt catattaata atggtattca tcagatcttg
    25021 cacggtattg gaaccgagca tcgggataat catcagcggg atgacgatca ggaactggat
    25081 ccacgctgcg cgcgccggaa cgccgttttc gttcagttcg acggttttct taccaaagat
    25141 gccttccggg atttcggaga agaaaatttt caccggcgtt gcggtccaca tcagcaggga
    25201 accgaacatc gcggtaaagg acaccagacc gacaaagcga ttcatcaacg cttccggcag
    25261 accaaaatac gccgccatgc cgtggaatac ctgcaccgat ccgccagtaa atttcaactc
    25321 tttgctgctg acgaagacgt taatcagcac cgaggaaacg gaatacagta cgccgataaa
    25381 aatcccggcg aggatgatca ctttaacgaa cgatttcgaa ccgcctttga cgtcgttaac
    25441 gtacaccgcg acggactccg cgccacctgc ggccataaag atccaggtgg taacgccgag
    25501 gaacgcccag ttgaagttcg ggatcatcgc gtcaacggtg atcggatcgg caggctgtac
    25561 gccgccaacc agtgccgtac ctgccagtaa aatgtaggag agcgttaaca gcagcatcag
    25621 cgttgaagtg acggaggtaa ttggccccag cattttcgcc ccgttggtgg aaacccaggt
    25681 ggagaaggcg aacagcacca tactgataat ggtggtggca accggcgtca taatatattc
    25741 gtagccgagg aaggcatacg aagcataagc aataacgcgc ggcaacagtg aggtgaaaaa
    25801 gaacaggttt acgaaccagt aggtataggc agtaataaat gcccaacgcc cgcccagcga
    25861 actttttacc cacgcgtaga caccggcttc tgagttttta tttaacgaaa caaattctgc
    25921 gatgatcaga cagaagggaa taaaataaaa aatcgtcgcg aggaaaaaca tcggtgccga
    25981 ggccagtcca agctcaatat tattattgat aacgttgtta aagctgtaaa cggcggcaaa
    26041 agtcagcgag agcaagccga atttgccgat tgtattacgt ttggtatcag acataacaca
    26101 tctccgaaaa tgagtagggt agcggtggcg gcaaactcct tttgccgccg tagttgttat
    26161 ttgttatgga aataaccatc ttcgatggtg actttgagaa ccactttttt gaccgcgtta
    26221 ttgcagataa accgatatgc ttcatggata tcgtaaataa cgatttgccc ttcgtggacc
    26281 tctacggttt ctccgcagcc ttttaaatat tcacggtcag tttcatcacg ataatattcc
    26341 acgacctgta atgtctcttt cggcgcatat tcaatttttt gctgcccttg caggtagtaa
    26401 tgcacttcaa aatagcgtcg atgcccggta aatagcgcat cggtcgcgga gtccgtctcc
    26461 acgcggtaag tcaatgagtc accgatggag tgggcgacgc caggctgaat gttgtcgata
    26521 ttttcaatcg cttcaacgca gcgttgccac ttcttgccag aggcgtaaat ctggcggaac
    26581 tgttctaagt tatcgatgat cctcattgct tattctcgct gtgcaaattc gtggaaaaga
    26641 accctgcgcc gaactcatac gacgccaggc tttgcgcggt agcttctccg ccagaaaccg
    26701 gcagcaacgt aaagccgtag ctgaagtcac ggaaccagac gcgccaggag tccagcacct
    26761 cgctgcccca ggagttggag ccgaggccaa gcagctggtg gtcgagattc agggtgatgt
    26821 catcactgcg ctgcaactcg ttacagtgct gggcagcgtg gatgttttcc tgggtatagc
    26881 gccaggcgct gaagttaatg gggcgctgcg gaacaaccag cagaccattg ccgtggcggt
    26941 tagtcagtgc cgtccagcgg acatgctgac ggttgccgtt gttctgtggg aagggatagt
    27001 tctcgaacat ggcatcgacg gtgctgcgcc agatatcgat gatgttagcc tgctggctgt
    27061 cggcgtagtt ttcgcccggt ccacgaccgt aatacgccac ctggtcgtat tcgccgttaa
    27121 tccccatggt gaaaccgatg cacggaatga tgtgcggata gtcgccgtaa cgctcgccgg
    27181 aaagcgccac gttaacctgg ccatcggcag cgatgcgcca gatgtaggtg cagcgcatcc
    27241 cgaagtcaaa caccggtggg gcaataaccg tgcggctgat gatcaacact tcaccatcgc
    27301 tctgttctac ggcaaagtcg cgcagatgtt cctgcatgat ctgcaaatga ttcggctgcc
    27361 acagcccttc gtactcctgc ttgtggttgt cgatcattgg cttgaagaag ttgatttttg
    27421 gctcgcgggt tagcagcgac tcgccattca cctgccagga tgtcggttta ccactcgttt
    27481 ttgagaaggt gatcgcaaag ttgtagccgc gaacagtaca gctcaaacga tcgtcttcca
    27541 gcgtcagcgg acgggcgtta ttcggtgcga aaggtacagg ctgcgcggtg ttttccttca
    27601 gcgggaactg ataagtggcg atagaatgcc cggcttcgct gtagcgggtg cgggaatctt
    27661 tggtcaccgt aatgttgaga aacgcttcgc gggcgtccag ctgtggcagc gtgatctgta
    27721 agggggcttc gctgttcggc gcaacgtcgc gcagtttaat ctgttgcgtc gcgagcgttt
    27781 caccttcggc gcgcacctct gcgtgcaggg tgtagtcatc aagcgtggta aaccacagat
    27841 tattttcgac tttcaactcg ccgcgagtca gatccagcgc gtggattttt actggcgcga
    27901 taacctgttt gtactctttc aggcccggtc ccggcgtctg atcggaatag atcaaaccat
    27961 caagacagaa gttatagttg ttgggatagt cgccgtagtc gccgccgaat ttataccaga
    28021 cattgccgtt gtcatcctgc gcctggatcc cgtggtcgca ccattcccag acataatgtc
    28081 cctgaatgca atcatgctta tagaagacgt tctggtactc cgtcagcccg cccggtccgt
    28141 tccccatcgc atgagcatat tcacagatga tgcgcggctt cggatgcggg tattcaccaa
    28201 actcattcat cagcggcacg cgggtgtaca tggtggaaat aatatcgacc acttcagcat
    28261 cgcgatcttc ttcgtaatgc accagtcgcg tgtcatccag cgccttcgcc gcatggtaca
    28321 tcgcgcggat gttacagcca tagccggatt cattgcccag cgaccagatg atgatcgacg
    28381 gatggttttt ctgcgcgtga atatggcgaa caatgcgctc gacgtaaact ttttcccact
    28441 gcggatcgtc ggtaatacgg ctgatatcgc cgacattagc aaagccgtgc gattcgacgt
    28501 cggtttccgc catcacaaac aggccgtaga tatcacacag ttcgtaaaaa cgtggatcgt
    28561 tcgggtagtg agcggtacgc acggagttga tgttgtgctg cttcatcaac tggagatctt
    28621 tctcgacgcg atccatccca acggcgcggc ctttgcgatg atcgttgtcg tggcggttga
    28681 cgccgtgcag catcacataa cggttattga tccagaacag accgtcgcgc actttgatat
    28741 cacggaagcc aactcgttgt ggcaccactt ccagaacgtt gccgtcggcg tctttcagcg
    28801 tcatgaccag atggtaaaga taaggggatt ctgctgacca ttgctgcggc tgttcgacag
    28861 taaaagcaaa actggcgctg gtcagttttt caattgccaa atgatcaatg gtgctgctgt
    28921 gcaccacgtg ttcgccatca aacagggtat attccagcgt cgtgacgaca ggggaggcgg
    28981 cgagattttc cagcaccact tcgcaggaaa gtgtggcatc gcaataggct tcgtcaaagt
    29041 cggtacgaac ggtgaaatcg ttaatatgcg tgagttgttt tccgatcaga taaacatcgc
    29101 ggaaaatccc cgccgaccac cacatatcct ggtcttccac gtaggtagag tccgcccact
    29161 gcatcacgcg cacacacaac aggttgtcgc cggttttaac catcgcgctg atgtcaaact
    29221 ctgcggtcag gcgactgcct ttgctgaacc ccacatactg accgttaacg tagacttcaa
    29281 aataggtttc gacaccgtca aatttgatca gcgtctgttt accctgccag ccgtcgctga
    29341 gggtgaaaat gcgttgatag gcaccggttg ggttatcgct ggggacaaac ggcacatcga
    29401 tggggaacgg aaaaccttcg tcggtatatt gcagtttgcc gtgaccttcc atttgccaca
    29461 tggcggggac ggtaatatgc ccccagtcag ccattaactc agaggtgaag gcttctggca
    29521 cttgcagcgg atggtcaaaa aagtggaaat tccactgacc gcttaagggc agaaacaggc
    29581 tgctggtttc acgggcaaaa gtacgagcct gcgcaacaga atcatatgaa aaaaagtacg
    29641 cacgcggcgc aagtcggttt tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc
    29701 ctcccggtag agagaaatcg gaaccattta tggtgatggt agtttgtgta attgctgggg
    29761 agtttagtaa aaattttact aaaaagtagc ggataaatgc atttacttta actggatcac
    29821 gaaaataaac ggcagcactg tccggcaaaa acgattgccg gacagcgtgc aaggggattt
    29881 aacgggtcgt gccgcgcagt tttaatttgc tgggaacgaa gaccaacagc ggcagcgcgc
    29941 gaccatcgcg ggctttttca tacaccaggt taacgccctg acttcccatc atctcggaat
    30001 ggatgcgcac ggtggagagc ggcggaaagg taaatcgcgc ggtggggata tcgttaacgc
    30061 tgataagcga aatatcctgt gggatgttca ggccacgttc atgaattgcc cgcagtacgc
    30121 cgatagcaat ggaatcggaa gcaacaaaca gtgccttcgg atagtcttcc cgcgccagca
    30181 tctgttttgc cagttcatag cccgacgaac tggaaaaacc gccgcgccag atatcctctt
    30241 cgcgtaccac gtgtttcaga cgaccatatt ccgcaaaggc gacctcacga atatccgcct
    30301 tgccaggcaa gtcttcaccg ccaataaaac caatacgatt aacgccctga ttgatataga
    30361 aatcgatgat ttctttactg atacgtgcca gatcgatatc caccgcatcg taaccgctgc
    30421 cgggttcgtg aaagtcgata aaacagatat tgtcggtcaa cgcacaggcg gcggcgcgca
    30481 gggcggacgt gggtttgccg acaattaaaa taccggtgac gtttttaatg tctggtaagc
    30541 cgttgtgttc ataacagttg gtcagttcga tcgccagctt ttcgcactga gtttcaatgc
    30601 cgtggcggat cgccagatag taaggatcgt tgatctccag ctcctgttgg tagctgtaga
    30661 tagccagaat atggtgttgg ttgactgcgc ctgtctggag tttacgggcg ctactggtct
    30721 tgtactccag cttttcggcg atctcgagaa tgcgatgttt cgtctcttct ttcacattca
    30781 atgtcggatc gtcattcaag accctggata ctgtcgccag ggatacgcca gcttcgattg
    30841 cgatgtcttt tagtgttgcc atgtttacct tttttacttt ccctgatgtg cgcccccatt
    30901 gtagaggaag tgcttatcgg gtcacctgtt ttcagtaaat aaagcgcgtt tctttgacgc
    30961 tgtaacggta acacagcggc ttgcgaatgt gatcacgcct gcattctcca tttttagcct
    31021 acaggattga gaaaaacttt tgctatagtt tagtaaaatt ttactcgaag ggaggaaaca
    31081 atggaaaccg tggcttacgc tgattttgca cgtctggaaa tgcgcgtcgg aaagattgtg
    31141 gaagtgaaac gccatgaaaa cgccgacaag ctgtacatcg tacaggttga tgtgggggaa
    31201 aaaacgctgc aaaccgtgac cagcctggtg ccgtactaca gcgaagaaga gctgatggga
    31261 aaaacagtgg tggtcttatg caatttgcaa aaagcgaaaa tgcgcggtga aacgtcggaa
    31321 tgcatgctgt tgtgtgcgga aaccgatgac ggcagtgaaa gcgtgctgtt aacgccggag
    31381 cggatgatgc ctgcgggcgt acgtatcgta taaaaagatc ggatggcgac gtcgtatcgc
    31441 catccgattt gatactacgc ttcttcgaca ctgactcgca tggccgccag cgccttacgc
    31501 gccgctttga tcacctgttc acactgttca atggtcagtg tcagtggcgg ttcaatgcgg
    31561 atcgttttag cgttgttgag tgttccggcc accaggacgc gctggcggaa catctcgctg
    31621 gcaaagttat agccgatttc gttatcaaca aactcaatcg ccatcaacat ccctttacca
    31681 cgcgcttcct gtaccagatc gggatattcc cgcgccagtt gacgaaaacc gtccagcaac
    31741 atatcgcctt tttgttcagc ctgagccggt aagttctgct ccagcaacac attgatggtc
    31801 gccagcgccg ccgcacaggc cagcgggttg ccgccaaagg tggtggtatg caggaacggg
    31861 ttgtcgaaca gaacggaaaa cacctcttca gtggcgatgg ttgcgccaat cggcatcacg
    31921 ccgccgccga gggcttttgc cagacacagg atgtccggct gtacgttctc atgctcgcag
    31981 gcgaacatct tgcccgtgcg ccccataccg gtttgcactt catcgaggat catcagtgcg
    32041 ccgaactcat cgcacagctt acgcacggcg gtgagatatc ccggcggcgg cagaatgacg
    32101 ccaccttcgc cctgaatcgg ttcgaggatc accgcagcaa catcatcacc ggtttttttg
    32161 cactcgttaa gagccgtgcg catggcttcg atattgccaa acggtacatg acgaaaccct
    32221 ggcagcagtg gcataaaagg tttgcggaag gtcgatttcg ccgtggccga cagcgcgcca
    32281 agtgatttac cgtggaacgc gccgctggtg gcaataaaag taaacttgcc gcgcggtgac
    32341 tgataagcct tcgccagctt cagcgccgct tcaacggatt cggtgccgct attacagaag
    32401 aagctgtatt tcagtttacc gggcgttagc gccgcaaggg ttttcgccag catcgcccgt
    32461 aacggatcga gcagctcctg gctgtgaagc ggttgtttag caagttgatt ctgtacggcg
    32521 gaaaccacaa ctggattacg gtgccccacg ttgaaaattc caaaacctcc caggcagtcg
    32581 ataaactcct gtccctgggt gtcgacaagc gtatttaaac cccccgcttg ccactctacg
    32641 gctccgtaat ccccgccagc ggtaacagat ttgcgatact ctaaaaaccc cggattgaca
    32701 tgctctttga agtattcaat cacctcccgg ttaagtgctt tcatctcctc atgatccagc
    32761 gttcgcttct caatgagatt cagggcgtgg gcgctgcacg ctaaagccga tgcgctcgaa
    32821 ggtaacctgt tcaaaatatg ctccggaggc tcgcgtatca catgataccg atttaagtat
    32881 tgcagggatt gcgccactcc ggctgcgatc gcgaaaatcg ggataaacac aaatgtggtt
    32941 atcatttgcg caatatttaa tgctatttgt taacatctgt tgttattttg cacggttttt
    33001 gtcgggtatg gatttggcga ttgctgcgca aatgcacact ttcagggcaa caactgcctg
    33061 atttttgggc aagataatta a
//
LOCUS       DAZUCC010000030        32988 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZUCC010000030 DAZUCC010000000
VERSION     DAZUCC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.030"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(378..668)
                     /gene="nadS"
                     /locus_tag="ACHY09_001252"
     CDS             complement(378..668)
                     /gene="nadS"
                     /locus_tag="ACHY09_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFS3884249.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(669..980)
                     /locus_tag="ACHY09_001253"
     CDS             complement(669..980)
                     /locus_tag="ACHY09_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3884250.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1209..2117
                     /locus_tag="ACHY09_001254"
     CDS             1209..2117
                     /locus_tag="ACHY09_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3884251.1"
                     /translation="MAQEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEAMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2181..3122)
                     /gene="fabY"
                     /locus_tag="ACHY09_001255"
                     /gene_synonym="yiiD"
     CDS             complement(2181..3122)
                     /gene="fabY"
                     /locus_tag="ACHY09_001255"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFS3884252.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3167..3604)
                     /gene="dtd"
                     /locus_tag="ACHY09_001256"
     CDS             complement(3167..3604)
                     /gene="dtd"
                     /locus_tag="ACHY09_001256"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS3884253.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDCA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3601..4473)
                     /gene="yihY"
                     /locus_tag="ACHY09_001257"
     CDS             complement(3601..4473)
                     /gene="yihY"
                     /locus_tag="ACHY09_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFS3884254.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4467..5066)
                     /gene="yihX"
                     /locus_tag="ACHY09_001258"
     CDS             complement(4467..5066)
                     /gene="yihX"
                     /locus_tag="ACHY09_001258"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFS3884255.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5207..6094)
                     /locus_tag="ACHY09_001259"
     CDS             complement(5207..6094)
                     /locus_tag="ACHY09_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFS3884256.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(6084..7433)
                     /locus_tag="ACHY09_001260"
     CDS             complement(6084..7433)
                     /locus_tag="ACHY09_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3884257.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7532..8965)
                     /locus_tag="ACHY09_001261"
     CDS             complement(7532..8965)
                     /locus_tag="ACHY09_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3884258.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9035..10045)
                     /locus_tag="ACHY09_001262"
     CDS             complement(9035..10045)
                     /locus_tag="ACHY09_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS3884259.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10078..10965)
                     /locus_tag="ACHY09_001263"
     CDS             complement(10078..10965)
                     /locus_tag="ACHY09_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFS3884260.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(10990..11868)
                     /gene="yihU"
                     /locus_tag="ACHY09_001264"
     CDS             complement(10990..11868)
                     /gene="yihU"
                     /locus_tag="ACHY09_001264"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFS3884261.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12041..12937
                     /locus_tag="ACHY09_001265"
     CDS             12041..12937
                     /locus_tag="ACHY09_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFS3884262.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            12977..13765
                     /locus_tag="ACHY09_001266"
     CDS             12977..13765
                     /locus_tag="ACHY09_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS3884263.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(13825..15090)
                     /gene="yihN"
                     /locus_tag="ACHY09_001267"
     CDS             complement(13825..15090)
                     /gene="yihN"
                     /locus_tag="ACHY09_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3884264.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(15192..16172)
                     /gene="yihM"
                     /locus_tag="ACHY09_001268"
     CDS             complement(15192..16172)
                     /gene="yihM"
                     /locus_tag="ACHY09_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFS3884265.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(16180..16890)
                     /gene="yihL"
                     /locus_tag="ACHY09_001269"
     CDS             complement(16180..16890)
                     /gene="yihL"
                     /locus_tag="ACHY09_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS3884266.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTRDIPETLVPLITVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(17107..18930)
                     /gene="typA"
                     /locus_tag="ACHY09_001270"
     CDS             complement(17107..18930)
                     /gene="typA"
                     /locus_tag="ACHY09_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFS3884267.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRSPKDD"
     gene            19302..20711
                     /gene="glnA"
                     /locus_tag="ACHY09_001271"
     CDS             19302..20711
                     /gene="glnA"
                     /locus_tag="ACHY09_001271"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFS3884268.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLA
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            20885..21934
                     /gene="glnL"
                     /locus_tag="ACHY09_001272"
     CDS             20885..21934
                     /gene="glnL"
                     /locus_tag="ACHY09_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFS3884269.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            21946..23355
                     /gene="glnG"
                     /locus_tag="ACHY09_001273"
     CDS             21946..23355
                     /gene="glnG"
                     /locus_tag="ACHY09_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFS3884270.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            23467..23577
                     /gene="yshB"
                     /locus_tag="ACHY09_001274"
     CDS             23467..23577
                     /gene="yshB"
                     /locus_tag="ACHY09_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFS3884271.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(23767..25140)
                     /gene="hemN"
                     /locus_tag="ACHY09_001275"
     CDS             complement(23767..25140)
                     /gene="hemN"
                     /locus_tag="ACHY09_001275"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFS3884272.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(25329..25838)
                     /gene="yihI"
                     /locus_tag="ACHY09_001276"
     CDS             complement(25329..25838)
                     /gene="yihI"
                     /locus_tag="ACHY09_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFS3884273.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(25957..26123)
                     /locus_tag="ACHY09_001277"
                     /pseudo
     CDS             complement(25957..26123)
                     /locus_tag="ACHY09_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26420..27052
                     /gene="yihA"
                     /locus_tag="ACHY09_001278"
     CDS             26420..27052
                     /gene="yihA"
                     /locus_tag="ACHY09_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS3884274.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(27376..30162)
                     /gene="polA"
                     /locus_tag="ACHY09_001279"
     CDS             complement(27376..30162)
                     /gene="polA"
                     /locus_tag="ACHY09_001279"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS3884275.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPVAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVVDEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            30526..31458
                     /gene="yihG"
                     /locus_tag="ACHY09_001280"
     CDS             30526..31458
                     /gene="yihG"
                     /locus_tag="ACHY09_001280"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFS3884276.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCESLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(31499..32929)
                     /gene="yihF"
                     /locus_tag="ACHY09_001281"
     CDS             complement(31499..32929)
                     /gene="yihF"
                     /locus_tag="ACHY09_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFS3884277.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRGNVIPALAAAKVKLVDNELTQPLFIAAKNKSPMEATLRFAFGGSFSTTLDVAP
                     AKYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDLVLELSPLNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQINIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFVIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLIFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8307 a         8459 c         7895 g         8327 t
ORIGIN      
        1 gattggatct ttatttgcat aatctgaatg gtgtagccga aaaatgtcag catcctaaca
       61 gcacccggat ttaaggtgaa attaatcatc catcacagac cggtcagtac tgctaccaac
      121 cggtcttaac ttgctcatcg ttataatttt gttggtaaca gttcgggaaa caaggaattc
      181 cttccggaac acccccggta tcatccgata gcattgacgt caatcatcgg cgcgacgatg
      241 agttttttta atcgcatcat agggtggcaa cggtcagcac gattacgggt catagcaaaa
      301 ctcctgagca ggtggcacta cgccgctttc acaggagttc tgaatgctgg tgctttctgt
      361 atggcataac catgcctcta acggttaagt gcttgcagca catgttgcgg atcattggca
      421 atcgctcgca gcaaagcctt tgcaggcccg gtcggtgaac gtcgcccctg ctcccagtta
      481 cgcaacgtgt ctacgcttac agaaataaga tcggcaaatt tagattgtga caaccctgaa
      541 gcgcggcgta tttctttgat tttcatagca tcaacttcaa aagtccgcga aggctgtcgc
      601 gttccttcaa taatttcatt catctgggcc atgctgtgag tcaaccgctc aaaaagtgcc
      661 ttatccattt accacctcgt atttaacaac cggagcattg ctttttcctg gggagacaga
      721 tcatctttaa tacccttacg ataaataagc agtaatctga tctcataatg tttaacctga
      781 tgaaagtaaa taaccctgac accagcacgt ttcccctttc ctcctgacac ccatcgaacc
      841 tttcttaacc caccagtttc aggtattacc tcaccatagt caggattcaa ggccaggaaa
      901 aactgaaaac gactgaattc atcgtcggtc agcagttttt gaacatcttc agtaaaaatt
      961 tccgtttcga tgaagagcat aattatacgc cattggctta gagtaacaat cctttttgta
     1021 cgtcattgac gtacccttga aaccactagc ttactgatag tgctccagca aaaccacgtc
     1081 tctgccgggt ttgtctttac atttgcgagg cgctttccag atttttcacg cccgtgaatt
     1141 tccctgtatc catccagtaa aaacgggtgt ataaaactgt acattcatac agcaaaatga
     1201 gttctactat ggcacaggaa aaaggaattg cagagttagt cgaagagttt atcgctgcgg
     1261 gtcgcccctc atcgcgtcaa caaagtattg cgcagcgcag ggagggctat atcgccagcg
     1321 ctgtccttgc gggtgagaca gaaacgcggg tagatattca gactatcgaa cttgaagcga
     1381 tgaccttacg aatagtgtca ccgctgaatg ccccgacatt actcccgacg ataatttatt
     1441 actacggtgg ctgttttgtt agcggcgggt tcgcaactca cgacaaccag ctgcgccagt
     1501 tagcctatta cgggcaatgt cgggttattg ccgtccagta tcggctggca ccggaacata
     1561 ctttccccgc cgcccatgat gatgcgcaac gaggtgcaga attagtctgg caacacgccg
     1621 aacgactcgg cgttgataaa caacggataa ctcttgccgg tgacagtgcg ggcggacatc
     1681 tggcgctggt aactgctttg cgattaaagc gtgctggcga gtggcaaccg gcacaactca
     1741 tcttaattta tcccatgctt gatgcgactg cgcatttcga aagctacata cgcaatggtc
     1801 acgattacat catcacacgc gacactttac tgagcggctt tgagatgtac ctgccaggca
     1861 ttgaacgcag gcatcctgaa gccagccctc tttggcgcga tgatttcaat ggcttgccac
     1921 ctgtacatat cattaccgct gaatacgacc cactttgcga tgaaggtgaa gcgttgtatc
     1981 accgcatgac agggcaagga gtgcaatgca cctgccaacg ctaccttggc gttatccacg
     2041 gattttttca gttaggtggt atcagtgagg ctgcgcgatc ggcgctacgg gatgttgcct
     2101 ggagagcggg aaggtaactc cggaccaggg cccagaaaag gttttcagat tgaaaacctt
     2161 ttcagtcaag taaaacgccc ctactcttct tcgttcccgc cctcttcata cgggccaaat
     2221 ggcttcgcgg gcagaacgat atacgtgcct tcaaacactg cacccggcgt ctcgtcgcca
     2281 aagatttcta cttgcatctg cacccgtgct tttcgtccgc gcgccagacg gtcgagatcg
     2341 ccgcttaagg caccaaggtc ggctaccgca tgaggtttgc cgctaatcgg cttgctgtag
     2401 cggatatgcg catccgccag aataatcgtt ccgccgaggt ggcgttcgcg cagcatcagc
     2461 cagataagcc cccaaccggt gagcgttgcc agcgagaata aactcccggc aaacagcgtg
     2521 tgatgcggat tctgattgcc tgtttccggc atggtggtga taaatttttg cccggtatat
     2581 tgctgaatgc gcacgcccat tttttcactc agcgggatgt gttcatacca cgcctgttgc
     2641 agctgcgcgc accagtcgcc gcgatgcaaa atatcatcca gagtggcaac gggtttaatc
     2701 atcaaaaaat ggcgaatcgg cgtggtggtt ggcgtggtga tttctccctg attaacaaac
     2761 cccagcttgg cgaaaaactc caccgcgtct tcgcgggcgc tacaggtcac gcgcttaaca
     2821 ccttcctgac gcgccacaga ttcgagggtc atcgccatca gcgttcctaa ccctttgtcc
     2881 tgcacgtcgg gatgaacggc cataaagcga atggacgctt cattgtcggc attaatatac
     2941 agtcggccta ccgccaccag attaccctgc tcgtcgacga ccatctggtg atgcgccatc
     3001 gcatcccacg cgtcgcgttc cgaacctttt ggttgatgca ggggcttacg caacatttcc
     3061 cagcgaaact gatagtaacg ctctaattct tcttctgttt gtggaacccg aaggtgatac
     3121 atagcgatac tctctcttgt ttcccgtgac aaccctggaa gctggctcat acctgcaacc
     3181 agaatgtcac ggggccatca ttgaccagcg atacctgcat atccgcagcg aagcgtcctg
     3241 tttgcgtgtt catctcttgc tgacggcagc gttcgacgaa atagtcatat aacgcctctg
     3301 cgcaatccgg tgatgcgcct ttggagaaac ttgggcgcat cccccgttcg gtatctgcgg
     3361 caagggtaaa ctgggaaacc accagtacac tgccgcccgc ctgttgcacg ttgagattca
     3421 tcttgccttc ggcatcgcta aagatgcggt agccgagcac acgctcgcac agacggtttg
     3481 ctttctgttc gtcgtcatcc ttttcgacac ccaataacac caaaagtccc gcgccaattt
     3541 cacccgtcac ttctccctcc acggtgacgc tggcacgggt tacgcgttga attaatgcaa
     3601 tcatggttcg tcgtcttctt cttgttcagc agcttgtttt aatttgcggt attccccgag
     3661 agtgacagta atttccgcgc caagcaagac gatacaccat gtccagtaga cccaaacaaa
     3721 gagaatgggg atcaccgcca gcacaccgta aatgagctga tatgatggga acatggtgat
     3781 ataaagcgcg aaacctttct ttcctgcttc gaacaggagc gcggcgacaa acgcgccgac
     3841 aatcgcgtcg cggttaggta cacggatggt aggaacgatg ctgtacagca gccagaaaga
     3901 gatccacgac aacagcagcg gaaaaatacg caacacatta tcgatgacag tattgagatc
     3961 gctcgcccag cgcagagaga gcagatagga gctgatcgcc agactggccc ctgccaacag
     4021 cggccccagc gttaaaatca tccagtacac ggcaaacgag taaatttttg gtcgcgctcg
     4081 tttactgcgc cagatggtat tcaacgcgct atcgatggag tacatcaaca ataacgccgt
     4141 gacgatcagc ccgcacgccc cgacggcggt cattttgttg gaattggcga caaactgttc
     4201 gatatatcgc tggataacat cgccagtagc aggcagaaag ttggcaaaaa taaagtgacg
     4261 caactgaatg ctgacgtcgg aaaacatggg aaaagcggcg aaaagcgcaa aaacaacggc
     4321 aaccagcggc actaatgaga gcaacgacac ataggcaagg ttacctgcca gggttgtcat
     4381 gttgtcctca tcaatgcgtt gccagagtag ttttagccag gcccatagtg gacgggtacg
     4441 atgcctggct ttgtcctgaa tggtttttag cataacacct tcgcgaaata gtccgggatg
     4501 gtggttttat ctttcaccag aatactagta atgcctagct gattagctcc ttctatatta
     4561 tcggcgttat cgtcgaaaaa gaccgtatcg tctggtgaaa aaccttctgc ctgcaaaaca
     4621 tgctgataaa ttcgtgcttc aggtttgcgc atacccaagt cttgcgacag atagatatgg
     4681 tcagcagcat cacggatttc cgggtattcc tccggccaga aggtagtatg caggcggtta
     4741 gtattggaaa gcaccaccac gcgatgcccc tgctcacgca gtttatgcat gatggcgatc
     4801 acttccgggc gcagcgcaac aaataccgcc tgccaaccgt gggagaactg ctcatagctt
     4861 agcggcaggg ccatctcatg gcacagtgcc tctgcgaacg cttcgtcgct aatttcccca
     4921 cgctcatgct gatgaaacgc ctcgcccatg tggaaactct tcttaagcgt agccagtgga
     4981 acacgcgtta aatcgctcca ggcccccagc acacggttaa agtcgatatc gacaatcaca
     5041 ttacctaaat caaagatata gagcattttt gcctcctttc gcgccacaag aaattaactg
     5101 tagcgggaaa ggatgggttt ggctatgtgg caataagggt aatcttgtga tgctgattgc
     5161 gaaaaagcag agtaatttca ctggcttttt cgcaatagat gaatggttaa gattcaggtg
     5221 taaattgcca acgagtttcg atgaccagcg gcttatctgg tgtcacaaat tccggcgtta
     5281 gatttattgc gttaggtgcc tgagtcagcg ggttaataca ggtggcatcg ggctgtttat
     5341 caaataccac cagactgttt gcagaagagg tcatggtcat cgcgagttta ccaggccaca
     5401 gtaacttaac cttcaccccg tcataaaaac caaaacagtc atcccaggga ccgacttgag
     5461 gagagatacg atttccagtt ggcagttcat cactacccgc ttcttcttgc cagtccgcat
     5521 caaaaagcac ctgcaacgat tctgtatttt gcggagtcag ctttttcgca aaccacggat
     5581 gccagcctgc ggaggccggg aatgtatcgg catatgaatg gacttccagt tgcaaaacca
     5641 gcgcatcgtt ctccagcaaa aaagtctgaa tcacttcccc ttgccagggc cacggtgaag
     5701 ccaggggcat acgtaaagtc agggaatctg ctgttttgtc gatgatttcc cacgttgagt
     5761 aacaagccat accatgcaac gcatgtggtg gtttattggc gggtaaagaa taacattgcc
     5821 caccagcatt cagcgtcgca ttccccagtc gccctgccca cggcaccatt gggaaacagc
     5881 catactgaaa agctctgcgc tgcggctgcc attggcgtaa tacttcagaa ccaaatgcct
     5941 ttaacgaggt tatcctggca ccatcatgcg gataaatctg caggtgaata ttgccttttt
     6001 ccagcgtcac taactctcct acaggcccag gccatggcgc gtcaaacgcc tgttgtagcg
     6061 ccaggtaatg ctcgtttttt cgttcagttt tcatgagatt gttccctgat gtagcggtaa
     6121 gaagcggttt cttgttcatc ggttggctca atcagccaga cgcaaacaaa ggaaagaact
     6181 gagacagcaa ataaaaacag agcgacatac cacggttttc ctccccctag cgccagcaaa
     6241 cttgaggcga taagggggct tagtccgcca agaatcgcgg aaaattgata agcaaaagaa
     6301 aggccggtat atcgtacttt ggtaccgaac atgcgggtga aaagtgttgg ctgcacggca
     6361 aacatcatcg tcggacctaa gttgtagcca aggatcatac tgcaccagat gatgagcgta
     6421 cttttgctat ccagtaataa gaagaaagga aacgcgaaca ggacacaaaa tcctgctccc
     6481 gacaaataca gacttttttg acctatacga tctgataacc atccaaccaa tggacaactg
     6541 aaaataccga tcgccgaggc aatcaacata cccgtcaggg gaatatctcg cgacaatgct
     6601 aattggctgg aaatatagac aataccaaag gcgttaataa tattagagga gacactttcc
     6661 gccagcctcg cacccagtgc caataaaata tttcgtggat gttttttgaa taattcaatc
     6721 aacggaggaa cggacttttc tttattattc gtcgtttttt gcttttgaaa atccagcgtt
     6781 tcttcggtat gcaggcggat aaacagccca acgatcaaca tcaccgcaga aagccaaaac
     6841 ggaatgcgcc atccccagga gagaaactgc tccgaagtaa gaaaatggtt acagagagca
     6901 aaaataccgg ttgccagcat aatacccaca gacgcagccg tttgcgggag tgaacctaaa
     6961 aatccgcgtc gggattcggg cgctgattct atggtcatca atgccgctcc gccgtactcg
     7021 ccaccaagac caaagccctg aatcaaacgc agcaccatta atacaagcgg tgcccaaatg
     7081 ccaatttcct gatacgtcgg aataaaacca atcaaaaatg tggagcaacc gacaatagcc
     7141 agcgtccaga taagtgttat ttttcgcccg attttatcgc caaaatgacc aaagacgatg
     7201 ccacctaaag gacgagtcag aaaaccaaca ccaaaggtcg caaaggaagc tataagacct
     7261 ataaaaggat cgctgtctgg ataaaacagc ggtgcaaata ccagtcctgc tgccgtgcca
     7321 aaaataaaga agtcatacca ttccattaac gcgcctgcga aactgcccat aacgacacgt
     7381 ctcatggctt taccggaaag cccttcacgc ggtgcgggta acttttcaga cataattttc
     7441 ctctcctggt aggcgagaaa tctgggatac gaaccactaa acgtgggcag tttgaattaa
     7501 tgcgcatcgg cttaaaccga tgcgcattaa attatttctt caattcagcc agaacctctg
     7561 cgtcctcagc atcaacattt gcacccagtg gttcccaatt aatcaataca caggtcagga
     7621 atcccaggaa tggcacgatt gccaaccagt aaaacgcttc agttttcaac gcaccccaca
     7681 gcatcggcca gaaaatgagt cctgcaattg cccctgtgcg cataatagcg cgggcaaatc
     7741 cgaccccagt agggcgaata gcgggaggat aagagagtgt ggcaatggtc atgcccagcc
     7801 caccaggacc cgctgaatgg aagaaaataa ttgatcccaa taaccctaaa gacagccagg
     7861 gattagtcgt tgccagtgcg cctaacgaca gcaatgctaa ggtcacaaca gcaaatccat
     7921 acatcgactg ccggcgagta cccagtcgtt ggagaataaa cgagccgatc atcccgcctg
     7981 tcaccccaca aagggcattc acgacggcag aaccggttaa cgcgccagta agcccgccac
     8041 ttattattcc cgccaacgta agaggaagat aaacacctac ggcgttatat tgccacgcct
     8101 gcatggttgc cacgacacag ccaagaatgg tgcgtttgcg gtaacgatca ttaaataaga
     8161 tgccatatcc accactgtat ttattctctg ctttttccga gaatttcgct tctgttgtac
     8221 ccgatgccgc aacgcgagcc tgaacaccat aacgttttcc cagaatgtcg cacgcttcct
     8281 ggtaacgccc gacgcgtgcg gcccacattg cggattcacc aataaagaag taacgcaaaa
     8341 tcatgaagat gactgcaaaa atagctccga tgaatattcc gtaacgccac aattgttctt
     8401 cagcgatacc cgacagaagc agcggcaaaa gcaccaaata aacaacggtt gtcgaaacat
     8461 accaggccat ttgccagagg ttgaccgacg tgccggtttt cttcgacgtt gaaccacgaa
     8521 gctctgccac ggcattggtt gccaaaggga agtcgatgcc taaaccaaag cccatgatga
     8581 cgcggcacac cagcacccac cagatactgg gagcaatagc taccgctgca gcaccgatgg
     8641 tgcacagccc catgccaata ataaatgccc gcttttgccc aaagcggttt attactcggt
     8701 tcgctaataa accgccaatt aacgcggcga ttaatactga tgcattgacg gttgcagcaa
     8761 gacccggcga aatgccgaat tgagcggcaa tgtatttatt accaaacccc accatcgccg
     8821 cctggtaggc atcaatcaat atgccgccca aagcaataaa aataacgccg atactggtgt
     8881 tgattgcaga gttcttatta acaatatcaa ttacgtcctg tggccgacta attgaataag
     8941 ttgcaggact ggtgatttga ctcatcgcag tgtccttttt aattgttgta gggtggcgct
     9001 gacgtgacgc agcgccagac agagatttag aaaatcattc cggcgcaaat tgcagcatga
     9061 cctttatgga gtcattttgc tgcgcgcgtt cgaatgccgc cgctgcatcc tcaatggcaa
     9121 ataaatcggt aaccagttca ctggtatcaa accgcccatc cgccagccat tcgataacag
     9181 catcgaaatc ttcagggatg tacatgccag agttgagcaa gtcacgctcg aaacgttgca
     9241 tcatcggtaa tggtatttca cgcggcccac ttggtacgcc catgcacacg acggtgcctc
     9301 cggcatatac acgggtacat gcatcgttaa gcgtggcctg cgctgcaacg acatcaatcg
     9361 cccccgtaaa tcgcacatct gcggccagct cacctggtgc ccaaacttct gctgcgccca
     9421 gttttaacgc cagcgcgcgt ttggcagcgt caggttcaat cacggtaatc ttacctgccc
     9481 ccataatgcg cagtgcctgc acgatggata agcctatcgt tccggcacca atcaccagta
     9541 cgctttccag agaagctttc ggcattcggt taacgcagtg acgagcacaa gccgccggtt
     9601 cggcaaaggc caacactttt agcggtaaag aggctggtgc gacatggcaa ttacgcgcag
     9661 gaacaatgtg ttgtgagcga gcaaagcccg gtgcgcggaa accagcaacc tgcggtggtt
     9721 cacaaagatt aaaacgtcct gctttgcagg ctcggcattc catgcaagcc atgatgggat
     9781 cgaccacaac atgatcgccc ggctgtacat ttttaacgtc gctgcccact tccgtaacgc
     9841 gcgctgcaat ttcatgaccg gggacgacag gcggtttggc aaacggatga ccgcctttca
     9901 gaacatgcag atccgaaccg catatcccgt agaacacggg cgcaatccgt acttcgtgcg
     9961 gtttaagttg ctggtgtggt acttcaacat attgagtggt gactttccca atatctgaaa
    10021 ataaaacctg ggtaatttta tccattgtta ttctccagaa tcgatatcca taggggatta
    10081 gcgttgcgcg ataccttcat cgacaatttc agccagccgt tgcaagcgtg gaacggagac
    10141 atcgcgcagc atggtttgtg gatcttgtgc accaacaacc gacgcccaaa ccgctcgtcc
    10201 agccaggaag cccgacgcac cacctttcat ggcaatactt actgcacggc caaaaatatc
    10261 ggcatcaacg ccagaagaaa gaattaccca cggcatcttc atctgatcgt taagttgctg
    10321 acaggcagca agtaaggttt tttcatcgcc tttgccccct aatggcatct cggctttgta
    10381 aagatcagcc tctgttccac caagctctgc ggcggcggca acaatggcgc tttcacgatc
    10441 aaagtcccaa ccgcgacgag gtggacgcac cacaggttca ataatgctaa ccagcccggc
    10501 actacggcag cgacgaacaa atttatcaac cattgccaga cgttcctctg cgggttcatc
    10561 ttcacgccag agtacaagta atttcattgc cttagccccc atttcacggg ctttatgcgg
    10621 gtcgacggac atatctattt caacgctatc aaccgggatg ccattacctg ggataaaaag
    10681 atcggcagcg acaatcaggc cgcaagaatt agccacagcg ccctgctcga ctatttgttc
    10741 aagacaaaat tgtttatccg ccagaacggc agaagcataa ggagaaagga ttcgggtcgc
    10801 ggcaacttta aaatcggtga gtacactgtc ggcgatgggt tttggctgcc ctgctgccgc
    10861 aaacatcagg cgcatggcct cacgctgatc gacagctaac atggcgaatc caccacctgg
    10921 gcgggagata tctttcagcg taaatggggt gaagtgggat gacatagaaa ctcctgttaa
    10981 tagataaaat taaagaccag cctgctggcg aacctgattt agcagggcgc tccagtcctg
    11041 ccgccctttg ccgtcttgac ttgctgcggc gtaataactt tctgctgcaa tccccatatt
    11101 gagcggcgcc cccgtttccc gagcgacgtc aactgcaata cgcagatctt tcaatgccag
    11161 gtccaccatg aaggcgggag aaagatcgcc tttcagcact ttgccaggcc aggtggtcgt
    11221 aaaatgtcct ttccctgctg ccgtaccact catcacctta attgcgacat caagatttaa
    11281 tcccagtgac tcacataaca cagcggcttc ggaggacagc gcattaagcg caatgctcat
    11341 atagttgtta attagcttca cgcggatccc cattcctggg ccaccagcct cgaccagctc
    11401 attacccatg cacatgagaa tcgggcgtgc acgcgcaatc tgctcatcag tgccgcccgc
    11461 taaaatcagc aatgtgccat caatcgcatt cactgacgtt ctgcccacgg gggcgtccat
    11521 catattgatg cctttttctt gtaattcccg tattaatgca tcggtctgta agggatgaat
    11581 agtcgacata tcaatcacta acgcgtcaga tgaaacagtt tcacagacgc ctttttcccc
    11641 caacagcacc tgtcgaacaa tgtccccatt gggaagcata gtgatgataa attcagcgct
    11701 tgctgccgct tccgctgggg tttgtgccgc agtggccccc tgcgtaacta gcgcatcgac
    11761 ggcctgtgta ttgacatcaa atacctgtaa tgaatgccct ttttgcaata gattgttagc
    11821 cattgggcta cccatctgcc ccagcccgag aaatgcaatg gttgccatga atactctcct
    11881 gtctgacatt gacaaaataa tgacaattga aaattcgttt tcgtctgttt ttgagcatat
    11941 ttgtaattta tgttcaaaaa cacgacacgc atcactaatt tgatcatttg tgtcatttaa
    12001 attgaccaaa gacgacaaaa ttaaacagga caagacaaat atgaccagaa ttgcatgcgt
    12061 gggtatcaca gttcaggaca ggatttattc tttgcccact ctgccggagg gcggcgggaa
    12121 atatcaggca aatcattatc ttgagattgg tggcggcccg gcagcaacag ccgctgttgc
    12181 aatcgcaaag cttggcgtgg aggttgattt catcggtcgc gtcggcgatg acagttgtgg
    12241 aaatacctta cttgcagaac ttgagggttg gggcgtcaat accgcatttt gccgccgata
    12301 tcctaacgcc cgttcgtcgc aatcagcgat cctcgttgat cagcatggcg aacggattat
    12361 cgttaattat ccaagtcctg atctcggtac tgatgccgaa tggttagaag cgatcgattt
    12421 ctctcgttat gatctgattc tggccgatgt tcgctggcat agcggtactg aaaaagcatt
    12481 ctcgctagcg cgccttgcgg gtgtgacaac gcttttagac gccgatatga caccgcagga
    12541 tatttctcca cttgtcgcgc ttgccgatca cgccgtgttc tcaacacctg ggctgaaacg
    12601 catgactggc ctgcagtcac ctgaagaagg tttgttccag gcaacgacac aaacagcagg
    12661 taaagtgtat gttacgctgg gttctgaagg atcgttgtgg attgaagatg gtcatttgtg
    12721 tcaacaggag gcattttctg tcaatgtggt tgataccact ggagcaggcg atgtttttca
    12781 cggcgcactc gcggtagcgt tggcagaaaa aatgccaaca aaagaggcta ttcgttttgc
    12841 cagtgcggtt gccgcgatga aatgtaccca gcctggcggt cgggccggca ttcctaatcg
    12901 tgagcaaacc gaatcatttt tgtcacttta tgcgtaaaat ggtaaacatc taccgaggag
    12961 gggcaaggga gtattcatga gtatcatcga agtgacaggt aatccacgcc atgaccaact
    13021 cgttcatcta atcgcagaac gcggttatat gaatattgaa gaactggcac aactgctgga
    13081 tgtttcaacg caaaccgtgc gccgggatat ccgaaaactc agtgagcagg gcttgatcac
    13141 ccgtcatcat ggaggggccg ggcgcgtctc cagcgtcatg aataccgctt ttgagcaacg
    13201 ggaactttcg ctcaccgccg agaaacgggc gatcgctgag gcagtcgccg attaccttcc
    13261 cgaacgctgt accgtcttta tcaccatcgg tacaaccgta gaagccgttg ccagggcatt
    13321 actcaaccgg cgtgatttac gcattatcac taatagcctg cgtgtggcac agattcttta
    13381 taagaatcag gatattgaag tgatggtgcc gggaggcact ttacgcgctc ataacggtgg
    13441 gattatcggc ccaggagccg tggattttat tgaaggcttc cgcgcagatt atttaatcac
    13501 cagtataggt gccattgaac atgacggcac cctactggaa tttgatgtca atgaagcgtt
    13561 agtcgcgaga acgatgatta aacatgcgcg gaatacattg ttagtggccg atcatacgaa
    13621 gtttaccgcg tctgctgccg tttcaattgg caatgcacgg aatgtcaggg ctttttttac
    13681 tgatgccccg cctcccaatt ctttctgcca gttgttaagt gaagagaatg ttgaactggt
    13741 ggttgccgag caagaagtat cctgaaattg aaacttcggt tgatacggtt ttcgttggcc
    13801 ggattaagca catccggcca ctaattaagc ttcagccgcc agcatctctt tcttacgttg
    13861 gctaatacgc ttaaccagca ttacggaaac caccgcacca cagaatgcga agcaggccat
    13921 aatgccaaac acgattttgt agccaataat ccccggattt aaatccagaa tgtagccata
    13981 cagactgaag cagaacatcg ccggggcgta accaataaag ctacccaacg ccatcgccgc
    14041 gcctgtttta ttttcagcaa tttttgcttc gccgataggt gcaaaaaata cggctcgctg
    14101 tgtgaagact atagcgccaa agcccagcgt acatgccatc cctaaataga ccggcatact
    14161 ttcgtgcggc agcataatca acagtacgag cgcagcggta cttatgataa aggtgtagca
    14221 tagatattta ctcggcgatt tcaggatctt atctgaaatc atgccaccaa tcggtcctcc
    14281 aatcattttc agacagtatt ggttaatgat gccgtaagcc cccaccagag caacgggcaa
    14341 tagatagatg tttttcagga atggaatgaa gaatgttagg ccacagtaaa ccgcgtaaac
    14401 gaagaagacg ttgaaagcga taagccagat ggttttatct ctcagcaccg aggtcatact
    14461 ggtgttttgc gatgctccgt cttctttttt cacctcaacg gacggtgcct cttctttgtc
    14521 attcaggaca aagaaaatga taatgcctac ggcaatcaca ataagggaat agaaccagat
    14581 gcctgcttta aaacccaata agccactgcc aaaccaggta aatactgcca acgcagaaaa
    14641 tgccaccacg gtatcgacaa tgccacgccc tgtttcgaag aagccaaaca accgaccttg
    14701 ttgttcgcta ttgcccaatc gacttaccga cttgagcaag accggccagt tcatcatgtc
    14761 gcaagtaacg ccaaatagcg cccagacaaa gaggatgccc caataccccg gcattgtcgt
    14821 caaataaaca cccagtaatc ctgtcgcaat gagtgagaat gacatggtgt atctgcgcgg
    14881 tagtttatcg gcaaaataaa tagacagaaa aaagcccact gtggtgacaa atgagtttac
    14941 cgacatagca ttaccaattt gaccattggt caaatggaaa tattcctgca tcgggatata
    15001 aaacgcatct ttcagagacg gtaatttata aattgtaccg ccacacaatg ttaatagact
    15061 aaataacgcc catttctttt tcgtgagcat aacatcccct aagcgatttt taaaatcact
    15121 gttttaaagg caataagata ccgaccattc atatggaatg gttttattaa cagcgtatga
    15181 gttaaaactt attagttgtt cagaagatgg tttgcctctt gtttgatttg ttgtagtaca
    15241 ttacgcacat gatttatctg attaattgca tcttcttttt ctttacgtaa ccgcgcatcc
    15301 agtaaatgat tttctggcaa tggtgtttca ctcatctggc gataagggat ccacggatta
    15361 tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat caacctcttc ttccaggcca
    15421 tatgccgtca cctgcggctc atcatcaccc aggcagataa ggtgcgttaa taacgctttg
    15481 aacggcatat caccctgacc tgaaatacag gctttatgac ccaggccacc ctcttcttta
    15541 acgatcaagg catctttgat atggacctgg gtaatatgcg gtgccatcgt ttttaaagcg
    15601 tcgatgggat gctcatttgc attgatcatg ttcgcaaaat caaacagtaa ggaaagagat
    15661 tccatctcac tttctttgac cagcgacacc agctcatggc tttttaaatc ttcatgctgt
    15721 tcgatcgtaa aagtcaggcc gctgtcctga tacgtttccc gtacataggc aatgtcgtta
    15781 gcgatgatcg ataatacgtc gcgcaaatta ccttcataac gtggataaaa acgtacggac
    15841 gatgccccag ttttcaacgc aatggcgacg gcttcgtcaa tagatgcctt atctgaagcg
    15901 ctggtttcaa tatgaatatc aaggttcaga cggcgggctt tatccccaaa ggcagagagt
    15961 tctttatcgt ccatattacc aagagaaaaa cgctcgccat ccagaacatg gattttgacg
    16021 ccacgtagat tattttcact tgcgatatca agtaaatcag caggcaacac tctttccagc
    16081 cgcatattta aatgaaaggc ataagcatgt aaataaagag gaagagtgtc gacacgttgt
    16141 agaatctttc ttgcgttatt tattgtcacc ataaaacacc tacagattaa tgatgaggtc
    16201 tacggcctca aatcgccaat attcaatatc tatctggaca atattgcctt gctggtcggc
    16261 gcgatgcttt tcaattaaga tcgctggcat acccgcagaa ccgccaagat acttgctgat
    16321 atcgcccggc attctgacgg gtttgaaaat caattttttt actaccgtct ctttttggta
    16381 gcacttttcc cagactgcag aaaatgagtg gttttcaagt tgttcaataa aacccggggc
    16441 aacttcagga ttaatatatg tttcgtgata gaaaacttta tgtccttcca gcgctcccca
    16501 gccggtgatg cgatagagtt cagttgatgg tgtcactgta atcagtggca cgagcgtctc
    16561 gggaatatct ctggtacggc ttttctcggt aaacccccag gaaggctccc gcccttgctc
    16621 aattgcagcc cgctgaaagc tcgccgacag ctctggactg taattaaatc gcggctgggt
    16681 cacgaaccac cctttacgat ccttacggaa gattttggat tctgcctcga ggttcaacaa
    16741 cgcctggcgc agcgtcatac gtttaatgcc cagcagttct cccagctctc tttctgaagg
    16801 gagttttcca cccggcgtgg tgatgccatc tttcaaccac cgatccagtt tctcttttgc
    16861 attttctacg gtggattgat tctccgccat tgtttaatcc ttttggttta aaccagatga
    16921 ggcatcttac gcttacgcca aaaaagaaag caattaacgg cgtatgactt tgcagataat
    16981 tgtgacaaag ctctctattg acgtaatcac agacttataa gggagatact cgcattggtg
    17041 tgcgctcacg acaggtaaaa aaaaacctgc cagcgatggc aggtttttta tgactgaaaa
    17101 gcgaaattaa tcgtctttcg gtgagcggtt ggcgcgacga cgatcgtttt ccgtcaggtg
    17161 acgtttacga atacggatag aggtcggagt cacttctacc agttcgtcat catcgatgaa
    17221 ctccagagct tgttccagag tcatgcggat aggcggaacc agaacaacgg cttcgtcagt
    17281 accggaagca cgcatgttgg tcagtttctt accggtcagg cagtttacag tcaggtcgtt
    17341 agagcggcta tgaataccga taatctgacc ttcgtaaact tctgcaccgt gaccgaggaa
    17401 cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg accgctttac cctgaccgtt
    17461 agagatcagt acaccgttct gacgctgacc cacttcgccc ggacgtacgt cgtcgtagtg
    17521 gctgaaggtg gagtacagca gaccagtacc ggaagtcatg gtcatgaact cagaacggaa
    17581 gccaatcaga ccacggcttg ggatcacgta gtcgagacgt acgcggcctt taccgtctgg
    17641 attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc tgcatcacag aaccctgatg
    17701 ctgttcttca acgtccagcg tcacgttttc atacggctct tgtttacgac cgtcgatttc
    17761 acggaagatt actttcggac gggataccgc cagttcgaaa ccttcacgac gcatgttttc
    17821 gataagaaca gacaggtgca gttcgccacg accagaaacg cggaacgcat cggcgtcttc
    17881 ggtttcttct acgcgcagcg caacgttgtg taccagttct ttgttcagac gatccaggat
    17941 ctgacgagac gttacgaact taccttcttt accgcagaac ggcgaggtgt taacgcagaa
    18001 gaacatagaa acggtcggct catcaacgga gagtgccggc agcgcttcaa cgttttgcgt
    18061 gtcgcaaacg gtgtcagaaa tgttcagttc gcccaggccc gtgatcgcca cgatatcgcc
    18121 agcttccgcc agatcggttt cgatacgttc cagaccgagg tggcccagca ctttaccgac
    18181 tttcgcgttg cgggttttgc cttcgctatc gatgatagta acctgctggt tcggcttcac
    18241 tttaccgcgc ttgatgcggc caatgccgat aacgccaaca tagctgttgt aatcgagctg
    18301 agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc ggcgcaggaa cgtggtcaac
    18361 aatcgcctga tacagcgggg tcatgtcttc cgccatatct tcgtggtcca gacccgcgat
    18421 accgttcagc gcagaagcgt aaacgatcgg gaagtccagc tgctcgtcgg tcgcgtcgag
    18481 gttaacgaac agatcgaata cctgatccac aacccaatca ggacgcgcgc cagggcggtc
    18541 aactttgttg ataacaacaa tcggcttcag gccgtaagca aacgcttttt tggttacgaa
    18601 gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc agcagcactg agtctaccat
    18661 ggacattaca cgttcaactt caccaccgaa gtcggcgtgc cccggggtat caacgatgtt
    18721 gatacggtaa tcattccatt tgatagcggt gtttttcgcg aggatggtaa tcccacgctc
    18781 tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt tcggcacgag agtcgaacgt
    18841 accggattgt tggagcagct tgtctaccag ggtggtttta ccatggtcta cgtgcgcgat
    18901 gatggcgata ttacgcaatt tttcgatcac aactttgcct caggcattag aaatagcgcg
    18961 ttattgtaca cggattaatc gcactacaaa acaggatcac aaacatcctc cgcaaacaag
    19021 tattgcagag tccctttgtg atcgctttca cggagcataa aaagggttat ccaaaggtca
    19081 ttgcaccaat atggtgctta atgtttccat tgaagcacta tattggtgca acattcacat
    19141 catggtgcag cccttttgca cgatgttaag catgataacg ccttttaggg gcaatttaaa
    19201 agttggcaca gttttcgctt tatcttttta cggcgacacg gccaaaataa ttgcagattt
    19261 cgttaccacg acgaccatga ccaatccagg agagttaaag tatgtccgct gaacacgtac
    19321 tgacgatgct gaacgagcac gaagtgaagt ttgttgattt gcgcttcacc gataccaaag
    19381 gtaaagaaca gcacgtcact atccctgctc atcaggtgaa tgctgaattc ttcgaagaag
    19441 gcaaaatgtt tgacggctcc tcgattggcg gctggaaagg cattaacgaa tccgacatgg
    19501 tgctgatgcc agacgcatcc accgcagtga ttgacccgtt cttcgccgat tctaccctga
    19561 ttatccgttg cgacatcctt gaacctggca ccctgcaagg ctatgaccgt gacccgcgct
    19621 ctattgcgaa gcgcgccgaa gattacctgc gttccactgg cattgccgac accgtactgt
    19681 tcgggccaga acctgaattc ttcctgttcg atgacatccg tttcggatca tctatctccg
    19741 gttctcacgt tgctatcgac gatatcgaag gcgcatggaa ctcctccacc caatacgaag
    19801 gtggtaacaa aggtcaccgt ccggcagtga aaggcggtta cttcccggtt ccgccggtag
    19861 actctgctca ggatattcgt tctgaaatgt gtctggtgat ggaacagatg ggtctggtgg
    19921 ttgaagccca tcaccacgaa gtagcgactg ctggtcagaa cgaagtggct acccgcttca
    19981 ataccatgac caaaaaagct gacgaaattc agatctacaa atatgttgtg cacaacgtag
    20041 cgcaccgctt cggtaaaacc gcgaccttta tgccaaaacc gatgttcggt gataacggct
    20101 ccggtatgca ctgccatatg tcgctggcta aaaatggcgt taacctgttc gcgggcgaca
    20161 aatatgcagg tctgtctgaa caggcgctgt actacattgg cggcgtcatc aaacacgcga
    20221 aagcgattaa cgccctggca aacccgacca ccaactctta caagcgtctg gtcccgggct
    20281 acgaagcacc ggtaatgctg gcttactctg cgcgtaaccg ttctgcgtct atccgtattc
    20341 cagtggtttc ttctccgaaa gcacgtcgta tcgaagtacg tttcccggac ccggcggcta
    20401 acccgtacct gtgctttgct gccctgctga tggccggtct tgacggtatc aagaacaaga
    20461 tccatccggg cgaagcgatg gacaaaaacc tgtatgacct gccgccggaa gaagcgaaag
    20521 agatcccaca ggttgctggt tctctggaag aagcactgaa cgaactggat ctggaccgtg
    20581 agttcctgaa agccggtggc gtgttcactg atgaagcgat cgatgcgtac atcgctctgc
    20641 gtcgcgaaga agatgaccgc gtgcgtatga ctccgcaccc ggtagagttt gagctgtact
    20701 acagcgtcta atagttgaag ttgtactacc cggcgcaaca acgccgggat ttagttgccg
    20761 tggaaacttt cagcccatct ctgcatgggc ttttttctcc gtcaattctc tgatgcttcg
    20821 cgctttttat ccgtaaaaag ctataatgca ctaaaatggt gcaacctgtt caggagactg
    20881 ctttatggca acaggcacgc agcccgatgc tgggcagatc ctcaactcgc tgattaacag
    20941 tattttgtta atcgatgaca acctggcgat ccattacgcc aaccccgccg cgcaacaact
    21001 gctcgcccaa agctcccgca aattgtttgg tacaccgtta ccggaactgt tgagctactt
    21061 ctcattaaat atcgagctga tgcaagaaag tctggaggcg gggcaaggtt ttactgataa
    21121 cgaagtgacg ctggtcatcg acgggcgctc gcatatcctt tctgtgacgg cccagcgtat
    21181 gccggacggc atgatcctgc tggagatggc accgatggat aaccagcgcc gcttaagtca
    21241 ggaacagcta cagcacgccc agcaggttgc tgcccgtgat ttagtgcgcg gcctggcaca
    21301 tgagattaaa aatccgcttg gcggtttacg tggcgcggcg cagctgctca gcaaagcatt
    21361 acctgacccg tcactactcg aatataccaa agtgattatc gaacaggcgg accggctgcg
    21421 aaatctggtc gaccgtctgt tgggaccgca actgcccggt acgcgcgtta ccgaaagtat
    21481 tcataaagta gctgaacgcg tggtgacgct ggtgtcgatg gaactgccgg acaacgtgcg
    21541 gttgattcgg gattacgacc ccagcctgcc ggaactggcg cacgacccgg atcaaattga
    21601 acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg ctggggccgg agggtggtga
    21661 aatcattctg cgtacccgca cagcgtttca actgacctta cacggcgagc gctatcggct
    21721 ggcggcgcga attgatgtgg aagataacgg gccaggtatt ccgcctcatt tgcaggatac
    21781 gctgttttac ccgatggtca gcggccgcga aggtggcacc gggcttggct tatccatcgc
    21841 ccgtaatttg attgatcagc attcaggcaa aattgaattt accagttggc caggtcatac
    21901 cgagttctcg gtttacctgc ctatcaggaa ataaaggtga cgtttatgca acgagggata
    21961 gtctgggtag tcgatgacga tagttccatc cgttgggtgc ttgaacgtgc gctcgctgga
    22021 gcgggtttaa cctgtacaac atttgagaac ggcgcggagg tactggaggc gctggcgagc
    22081 aaaacgccgg atgtgctgct ttcagatatc cgtatgccgg gaatggacgg gctggcgctg
    22141 ctcaagcaga ttaaacagcg ccatccgatg cttccggtca tcattatgac cgcacattcc
    22201 gatctggatg ctgccgtcag cgcctatcaa caaggggcat ttgattatct gcccaaaccg
    22261 tttgatatcg acgaagccgt ggcgctggtt gagcgcgcca tcagtcatta ccaggaacag
    22321 cagcagccgc gtaatgttca gcttaacggc ccaacgaccg atatcatcgg tgaagcgcca
    22381 gccatgcagg acgtgttccg gattatcggt cggctttcgc gttcttctat tagcgtgctg
    22441 attaacggcg agtccggcac cggtaaagaa ctggtcgctc atgccctgca tcgccacagt
    22501 ccgcgagcca aagcgccgtt tatcgcgctc aatatggcgg ctatcccgaa ggatttgatc
    22561 gaatccgaac tgtttggtca cgagaaaggc gcatttaccg gcgcgaatac cattcgtcag
    22621 gggcgttttg aacaggctga tggtggcaca ttattcctcg atgaaattgg cgatatgccg
    22681 ctggatgtgc agacgcgttt gctgcgcgta ctggcagatg gtcagtttta tcgcgttggc
    22741 ggctatgcgc cggtgaaagt agatgtgcgg attatcgctg ccactcacca gaatcttgaa
    22801 cagcgggtgc aggaaggtaa gttccgtgag gatctgttcc accgcctgaa cgtcatccgc
    22861 gttcatctgc cgccactgcg tgagcgtcgg gaagatattc cccgtctggc acgccatttt
    22921 ttacaggttg ccgcgcgcga actgggcgta gaagcgaagt tgctgcatcc ggaaaccgaa
    22981 gccgcgctga cgcgcctggc gtggccgggg aacgtgcgcc agctggaaaa cacctgtcgc
    23041 tggctaacgg tgatggccgc cgggcaggaa gtgttgattc aggatttgcc cggtgaactg
    23101 tttgaatcaa cggttgcgga gagtacttct caaatgcaac cggacagttg ggcgacactt
    23161 ttagcgcagt gggcagacag agcgctgcgt tccggtcatc aaaatctgct ttccgaagcg
    23221 cagcctgagc tggagcggac gttactgacg accgcgttgc gacatacgca agggcataaa
    23281 caggaagcgg cgcggctact cggctggggg cgtaacactc tgacgcggaa gttaaaagag
    23341 ctggggatgg agtgattcac ggcttgtgtg taaagattga ttattgagcg caaattgctg
    23401 gtgttttgcg ctttactgtt ccgataagtt cagtatgatc ttgcccggaa aacggggaga
    23461 gtcattatgc tggaatcaat aattaatctg gtatcgagtg gcgcagttga cagccacaca
    23521 ccgcaaactg ctgttgctgc cgtgctgtgt gccgcgatga ttgggctgtt tagttgagtt
    23581 tcagattgct gatgaagtgc tctttgttca tgccggatgc ggcgtgaacg ccttatccgg
    23641 cctacaaaag caatattttc aacagatttt gcaggcctga taagcgtagc gcatcaggca
    23701 atcttgttct cagcacattg atggcgcttt cgttttctac tttgtaaacg aagcgccatt
    23761 cacaatttaa atcacccgag agaactgctg catccgcgct ttctggcgca gataggtatc
    23821 aaagcacatg caaatgttgc ggatcagcaa gcgacctttc gccgtcacct gtattccctt
    23881 ctcatccaca tccaccagcc catcttttgc taacggggcg agcagcttga gatcttcggc
    23941 aaagtaatca gcgaagagca aatcccactg ttgctcaatg ggagcgtaat ccagacggaa
    24001 gttgcagatg agcgacttaa tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat
    24061 accacgccac agcgcattgc cttgttcatc cacttgctga tagtactgct tcaactcttt
    24121 ctggttctgc gcgtagcagt cgccaatcat gctgatggcg gaaacgccca tccccagcag
    24181 atcggtatcg ccctgagtgg tgtagccctg gaagttacga tgcagcacgc cttcacgctg
    24241 ggccaccgcc agctcgtcat ccggacgggc aaagtgatcc ataccgataa actgatagcc
    24301 cgattgtgtc aggaaggcga tggtttcctg caggatatcg agtttttgct gcggactcgg
    24361 caggtcagca tctttgattt tgcgctgagc agcaaaaatg gtcggcagat gcgcgtagtt
    24421 aaagacactc agacgatcgg ggttcagctc cgccacacgt ttcagggtaa aggcgaaact
    24481 ctccggcgtc tgtttcggca ggccgtaaat caggtcgatg ttggtggagg taaagccaat
    24541 ctcacgcgca tggttaagca gtgcaaagat gaactcttca tcctgctcgc ggttaaccag
    24601 acgttgcact tctttgttga agtcctgcac gcccatgctc aggcgattaa agccttcggc
    24661 gcgtaaatga tcgagtacat ccagttcgat ttcccgcgga tcgacttcga tcgagatctc
    24721 cgcatcggca ttgaactgga agttttcgcg cagcagcttc atcagacggc tgatttgcgc
    24781 tttattcaga tacgtcggcg ttccgccgcc ccagtgcaat tggctgacgt gacgcccggc
    24841 gaacagcggt gcacgatgga cgatttcttg ctccagcgcg tccagatact gatcggcctt
    24901 gtgctgctgg cgagtaacta tcttattgca accgcagaag taacaaagct tatggcagaa
    24961 cggaatatgt acgtagagag ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa
    25021 cgcctgttcg ccgaagtctt ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg
    25081 cccggaatag ttatatttct ggatcagggc cagatcccag tcgatttgct gtacagacat
    25141 gctcactcct tccgatggcg tctctggcgg ctacggcgta ccggcggatg cccgtttcga
    25201 gtcatcagcc ggttgcgcag ccacttttgt cgccgcgaca accgctgtag tttaacgaat
    25261 aaccacacca gataacatat aaccggaagg gttatcagca gaacgggaag gcccacggcg
    25321 gcaatccgtt agttgccccg cagcagacgc atcatgtctt cttgcttctc gtcttcttcc
    25381 tcttcttcgt catcgtcata agagaggccg agtttctgca tcaactcatc aatacgatcc
    25441 agtttggcat ccacccagga ttgctcttcg gcgctcaggg tttcgcctgc ttccagacgt
    25501 tccagcagcg catccagacg ctcatccgtt tccagtaact ccaactccgc ctgcggtgaa
    25561 agcataggtt tctcactctt cggtttgtgc tgtttggtga ctttttcagt cacgcccaat
    25621 ggaatagggg ttttactgcc aatacgtgga tctttcggtg cgttctggcc tttgctgcct
    25681 gacgtggtgt taccgcccgc tgcgcggctg cccggcgcat gaccacgacg ttttttctga
    25741 cgcttacggt cacgagcttc ctgatccagc tcctcgcgtg tttttcgacg cgcttttgca
    25801 tgacctttgc tgcgtgagtt tgaagatgat ggtttcataa tggttcgtct tttagccgtt
    25861 ttattcagta tagatttgcg gcggaatcta acagaaagca agcaaagaaa aaaggcgaca
    25921 gattactctg tcgccttttt tcctgactca taacccttaa cgggtcttac aatccttgca
    25981 ggccgccaac acaccctgtt tttccattag ccggaacaat ccgtgttgat tccatttccg
    26041 tttaattacg tctccggacg tttatcttcc tgacaatcct ctgtcttcgc ctcctggcgc
    26101 tcctgaccct catcctgagt cattgttcct gttagcgtcc tcgctctatg ggtgctactt
    26161 tacgcctttg ctttaaataa acaatcaacc gccatcagta tttgcacatt ttcagagttt
    26221 tatataaaag caattcactg atttatatag cttatttgac atacgtcgca gaattttctc
    26281 tgctatttcg cataaagact atcggcaatg tctcacaaag catatggctt ttacttacaa
    26341 cgctcacgcc gaaaaatccg ccttttatta tggttcaggt ataatcgccg caaagctttg
    26401 actgatggag acgaccgctt tgactaattt gaattatcaa cagacgcatt ttgtgatgag
    26461 tgcgcctgat attcgccacc taccttccga taccggaatt gaagtggctt ttgcaggccg
    26521 ttccaacgca ggtaaatcca gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg
    26581 tacctcaaaa accccggggc gcacccagct tatcaacctg tttgaagtgg ctgacggcaa
    26641 gcgtctggtt gacttgcctg ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa
    26701 atggcagcgc gcgctcggcg agtacctcga aaaacgtcag agcctgcaag gtctggtggt
    26761 gctaatggat attcgccatc cgctgaaaga tttggatcag cagatgattg agtgggcggt
    26821 agacagcaat atcgccgtgc tggtactgct gaccaaagcg gacaaactgg caagcggcgc
    26881 acgtaaagcg caattgaata tggtgcgtga agcggtgctg gcgtttaacg gtgatgtgca
    26941 ggttgaaacg ttttcttcgc tgaagaaaca aggcgtggac aagctgcggc agaaactgga
    27001 tacctggttt agcgagatgc agcctgtaga agaaacgcag gacggcgaat aattttcttg
    27061 ccttaatgct tgtgccggat gtggcgtatc aggcattacg gatcttttct ttcgcccaat
    27121 aaaaaacgcc ccagtcatta ctgactgggg cggctaaaat attcagccaa atccgattac
    27181 gtgaagtaaa aggtctgaaa gatagaacat cttacctctg taccctacgc gactaacttt
    27241 actctttttt gttcagttca gaaagcattt tttgtaattt tttttcagtt gttgcatagg
    27301 aatttctaat agccatcaca aaacgtgaca gcttatgttg cttacttacg aaaaaagggc
    27361 atgttcaggc gaatcttagt gcgcctgatc ccagttttcg ccactcccca cttccaccag
    27421 cagcggcaca tccagacggg tacagttttc catcagttga tgaatctgct tcgcgacggc
    27481 atcgacatca tctttatgaa cttcaaatac cagttcatcg tgtacctgca tgatcatacg
    27541 cacacgcggt tgctcagcct gtagccacgc atcaacggca atcatcgccc gtttgataat
    27601 gtcggcggcg gttccctgca ttggcgcgtt aatggctgca cgttcagccg ctgcacgacg
    27661 agccccattg ctggatttga tatccggcag atacagacgg cgtccgtcca gcgtttcaac
    27721 gtagccctgc tctttcgcct gagcacgggt gcgttccata tactccagca cgccagggta
    27781 gcgttcgaag taaaggtcca tgtacttctg cgcttcttta cgtggaatgt tcaattgccg
    27841 cgccagaccg aaagcactca tgccataaat cagaccaaag ttgatcgctt tcgcgctacg
    27901 gcgttgctcg ctggttacgg tttccagtgg caaaccaaac acttctgccg ccgtagcccg
    27961 gtggatatct tttccttccg cgaatgcggt cagcaagcct ttgtcacgcg agagatgcgc
    28021 cataatgcgc agttcaatct gcgagtagtc cgccgagaca atcacataat cctctggcgc
    28081 aataaacgcc tggcggatac gacgaccttc ttcgttacgc accggaatgt tttgcaggtt
    28141 aggatcggtt gacgataaac gtcccgttgc agttactgcc tggtgataag aggtatgcac
    28201 acgcccggtt ttcgggttga tcatcagcgg cagcttgtcg gtgtaagtcg atttcagctt
    28261 cgccagacca cgatactcca gaatcacttt tggcaacgga tagtccagcg ccagttcttc
    28321 cagtacctct tccgacgttg acggcgcgcc acccggcgtt ttcttcagcg gtttaattcc
    28381 ctgtttttca aacagaatgg tttgcaactg cttggtggag gaaaggttaa attcttcacc
    28441 tgcaatttca tgcgctttct tttccagctc agccagacga agagtgagct cttcagaatg
    28501 attgtgcagc actttcggat cgatcttcac accgttacgt tcaatgcgtg aaagcaccgg
    28561 cactagcggc atttcgatat tctcgaagac gttcaacggc cctttgtgtt tttgcagatc
    28621 cggccacatt ttcagatgca actgcaaggt gacatctgca tcttcggcgg cgtaacgtcc
    28681 ggcctcttcg agggcaatct ggttaaaggt cagttgattt ttgcctttac ccgcaatctc
    28741 ttcaaaagtg atggttttgt gcttcaacca acgttccgcg aggctgtcca tatcgtgacg
    28801 cccggcaacg ctattgagaa tgtaggactc cagcatggta tcaaacgcaa tcccacgcag
    28861 ctcaatgccg taattcgcca gaataccgcg atcgtatttc aggttttgcc caaccttcag
    28921 cgccttttca tcttccagca gcggttttag caactcgagt gcacgctcgc gagagatttg
    28981 atcgggcgca tcaagataat catgagcaac cggaatatat gccgctacgc ctggctcgat
    29041 agcaaaagaa agcccgacca ggttagcaga gatgttatca aggctgtcgg tttcggtatc
    29101 aaatgcaaat accggcgctt tttccagctt cgcaatccac gctttcagtg tttcttcatc
    29161 aaggatggtg acgtagttgt cataagaaat caccgttgcc gtcacttctg gcgcttcgtc
    29221 tacaacactg gtttcctgcg gcttcgcggc tggttttgcc cctttggcct gtaaccattt
    29281 gcccgcttcg acatcagcag tccagcgttt gaactcatac tttttgaaca gccctaacaa
    29341 ctcttctgcc accggtggct gcacttccag ttgttcacag gtcagctcca gttcaacgtc
    29401 ggttttaatc gtcgccagct ggtaagagag ataagccact tctttgtttt gctcgagctt
    29461 cgccgccatt gttttcgcgc cacggaagct caacccagca attttttctg gctcggcata
    29521 cagcgtatcc agtccgccaa gaccttgtag caatgcttgc gcggtttttt caccgacgcc
    29581 cggtacgcca gggatgttat cagaggagtc acccatcagc gccaggaagt cgatgatcag
    29641 ttctggcggc acgccgtact tattcaccac ctcttccggg ccgaggatgg tattcgtcat
    29701 ggtattgata agcgtaatat ttggcgtcac cagctgcgcc atatctttat cgcctgtgct
    29761 tatcagcacc ggacgcccgg ctttttcggc ttcccgcgcc agagtaccga taacgtcgtc
    29821 cgcttctacg ccagaaaccg ccagcagcgg cagtcccatc gctttaacca tcgcgtgcaa
    29881 gggttcgatt tgtgcacgca gatcgtccgg cattggcggg cgatgtgatt tgtaatgttc
    29941 aaacagttca tcacgaaagg tttttccctt ggcgtcaaag accaccgctg catgcgtcgg
    30001 tttatattgc atgattagac tgcgcagcat gttgaggaca ccatacatcg caccggtcgg
    30061 ctcgcctgcg ctgttagtca gcgggggaaa cgcgtgatat gcgcgataaa gataagatga
    30121 accatctaca aggataagtg gattttgggg gatctgaacc ataatgtccg tgcctgttta
    30181 tcagattatg ggtaaaggat gccacagaca ggatgaaaac atgagttttt caccgctttt
    30241 cgtgaaaatc ttttgtagat cttctggatc gctcgcaaat cgtcatgtgg ataactttgt
    30301 gcataaatta tttacatcgc ttttaattta ttatcatgct ttttacagca tgatatttgt
    30361 ctttgctttt catacagtta aatataaagc catccctaat aattgtgata atgggatttt
    30421 ttctaatgtg gatatatatg aattattttt ctccttaagg atcatccgtt atttggggcg
    30481 ttttctgcta aaatcggcgc cttgcgcttc tttagcgcac tgtctatggc taaccttttg
    30541 aataaattca ttatgacgag aatactcgct gcgataaccc ttttgctgag tatcgtattg
    30601 accattttgg tcactatttt ttgctccgtc ccgatcatca ttgccgggat tgtaaaacta
    30661 ttgctacctg tgccagtcat ttggcgaaag gtttcgcgtt tttgtgattt tatgatgtat
    30721 tgctggtgtg aaagtctggc ggtattactg catctgaacc cacacctgca atgggaagtt
    30781 cacgggctgg aagggttaag taagaagaac tggtatctgc ttatttgtaa ccaccgtagt
    30841 tgggcagata ttgtcgtact gtgcgtgctg tttcgtaagc acattccgat gaataaatat
    30901 ttcctcaagc agcagcttgc ctgggtgcca tttcttggcc tggcatgctg ggcgctggat
    30961 atgccgttta tgaagcgtta ttcccgcgct tatttattac gtcatccgga acgacgtggt
    31021 aaagatgttg aaaccacccg gcgttcttgt gaaaagtttc gcctgcaccc caccaccatt
    31081 gtcaatttcg ttgaaggctc ccgtttcacg caagaaaaac atcagcaaac gcactccact
    31141 tttcaaaact tgttgccacc aaaggcggca ggcattgcga tggcgctaaa tgtactgggt
    31201 aaacaattcg ataaactgct taatgttacg ctgtgttatc cggacaataa ccgtcagccg
    31261 ttcttcgata tgttaagcgg taaactgacg cgaattgtcg tacatgtgga tttgcagcct
    31321 atttccgatg agttacatgg cgactacatc aatgacaaaa gctttaagcg ccattttcag
    31381 caatggctga actcactgtg gcaggaaaaa gaccggcttc tgacgtcgtt gatgtcatca
    31441 cagcggcagg acaagtaaat tttgctcgcc tctgaatgag cagaggcgag tgagtatttt
    31501 aatgaacaaa acgtccggca cgagacataa attcttcttc gctcatctcc tgtccgttaa
    31561 aaacaacttt acccggtgta taacgcagtt gcagcgaggc ggtattgttg tcaatcgtga
    31621 ttaactgaaa catctgacct aatgtcatca tcccgctgat ttgtttatca gcccgctttt
    31681 gcgctttttc agcatccatt ccttcagata aattaagctg ttttgcggtt tctgtgacaa
    31741 cattaagcgg taacttcaca tcaaaaatga gcgatttaat atctttgttt gtggatgatg
    31801 aggatttggc tgggtcggca atactgatat ccagattggc atttagttcg ccgagtgcat
    31861 tcttccatct taccggttgt ttaatggtcg gctcactttt ttgtaataac ggtaaatact
    31921 ctttgaacaa tgcagcattc acttcttgca gagcaacttc atcgtttgct aactcagggt
    31981 gtgcagcaag ctgcttctgc atcgcaatgt tatactggat gacaaattgg cgcaccgctg
    32041 agggatcaat agattcagca atcaatgacc actcaccact accgagttgc tgattccctt
    32101 ttgttaactt atcaagttcg taggtcagat tgacattgat ataatctttg tctttaacgc
    32161 gatccagcct ggtttttgca acgaaagcat cgatttgggc aacgtcttct ccctgattga
    32221 taatgttaat ctgattgaat ttcgactcgc tttcgccaac cggaaatttc ttttcttcta
    32281 atcgcgttag agaatcaatg gtaaaacttt tcgccgttac tttattcaat ggcgataatt
    32341 ccagaacaag atcttcgact ttgccttcaa tatccagatt agacaatgaa ctaccatcac
    32401 cattaaaagt aaactggccc tcaccaaaag aaaactttcc atattttgca ggggcaacat
    32461 ctaatgtcgt ggagaatgag ccaccaaacg cgaatcgcaa tgtcgcttcc ataggagatt
    32521 tattttttgc ggcgataaat agcggttgtg tcagttcatt atccactaat ttcacttttg
    32581 ccgctgccag tgcggggatg acattaccac gcattaacat tgtgatgggt aacggaccgt
    32641 gctcaacatc cacatcaaac gcaactttct ggccaggttt gatattgaga tcaggtgcac
    32701 cgttatcaaa tgtcatttgc atttgaaaac gagaattaaa aaagcctttg tcaaaatttt
    32761 tataacttaa cgtcatatcg taggcatgtt cacctttctt tttagcatcg ttaaaatctt
    32821 taataaactt ttcgatacca ggctgaattt gcgtacctgt gtaccatgtg ccaccacccc
    32881 agattacggc taacgcaaca ataacacctg tagctgactt acgtatcata taaatattcc
    32941 ctgataaaac agcaaaactt tgaatatcca cttatgctga aataatgt
//
LOCUS       DAZUCC010000031        32860 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZUCC010000031 DAZUCC010000000
VERSION     DAZUCC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.031"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..341
                     /locus_tag="ACHY09_001282"
     CDS             111..341
                     /locus_tag="ACHY09_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3884278.1"
                     /translation="MARNEVKPDWHPQRILGEVRAREGSMRALARKAGVGQNNLYNAV
                     YRHVPKYEGIIAECLGVPPEEIWPSRYIRKVA"
     gene            344..2431
                     /locus_tag="ACHY09_001283"
     CDS             344..2431
                     /locus_tag="ACHY09_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFS3884279.1"
                     /translation="MFASVKELVGLPGMPGTEQGVRYSIRKYATSETSRRRQEGSKGF
                     EYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSSDVVDAVEAYRGSPQL
                     MVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERIV
                     AMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWLP
                     EFLAQYRQSNGRPMTEAYEDFVAEWRLRHADEPYMLDIMPSYDTIRRAMKKLPEVVKQ
                     KGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKMKCRHPVHGRPFAPEVTFVID
                     GGTRFVVGWSMDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGIL
                     PRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLRS
                     AFTALQQGKRLNARQQSAMRDLPSWAELIDAIRDGVEWYNNRPHDELPIKPNGKHYSP
                     AEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHGR
                     KVLISYDIHDAERITVRRLDGSVICEAVWGGNKREAFPVSAEYYKQQQRLKGMRKRAE
                     EKIRDAEDEVVNVLEHKQQEPWLENIYRPVGNAVIVQQPVADDEPDEEYERNFQRGLQ
                     LLEAKLKENDPLA"
     gene            2503..3435
                     /locus_tag="ACHY09_001284"
     CDS             2503..3435
                     /locus_tag="ACHY09_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3884280.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            3438..3659
                     /locus_tag="ACHY09_001285"
     CDS             3438..3659
                     /locus_tag="ACHY09_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884281.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            3672..3926
                     /locus_tag="ACHY09_001286"
     CDS             3672..3926
                     /locus_tag="ACHY09_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884282.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            3928..4209
                     /locus_tag="ACHY09_001287"
     CDS             3928..4209
                     /locus_tag="ACHY09_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFS3884283.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            4206..4478
                     /locus_tag="ACHY09_001288"
     CDS             4206..4478
                     /locus_tag="ACHY09_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884284.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGISLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            4483..4776
                     /locus_tag="ACHY09_001289"
     CDS             4483..4776
                     /locus_tag="ACHY09_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884285.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCDVYVSILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            4788..5318
                     /locus_tag="ACHY09_001290"
     CDS             4788..5318
                     /locus_tag="ACHY09_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFS3884286.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            5416..5958
                     /locus_tag="ACHY09_001291"
     CDS             5416..5958
                     /locus_tag="ACHY09_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884287.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIET
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNTDSWWTPSLSRDALCVVFKVGAYGREIKG
                     CIPEECQEIKHSEYVALTEE"
     gene            5962..6495
                     /locus_tag="ACHY09_001292"
     CDS             5962..6495
                     /locus_tag="ACHY09_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884288.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            6495..7010
                     /locus_tag="ACHY09_001293"
     CDS             6495..7010
                     /locus_tag="ACHY09_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884289.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQTVKDEAKRLEAVLNVACRSVFE
                     FSISGFCFKGLRFTEDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVEWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            7014..7565
                     /locus_tag="ACHY09_001294"
     CDS             7014..7565
                     /locus_tag="ACHY09_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFS3884290.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRI
                     KRSFWGVHNRIVLLRGTYPELLKCKRLRFKPDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     GTVSFIYHRRRTAEDVVLRELMP"
     gene            7562..7873
                     /locus_tag="ACHY09_001295"
     CDS             7562..7873
                     /locus_tag="ACHY09_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884291.1"
                     /translation="MITTLFVESDEPLVCAVGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            7888..8238
                     /locus_tag="ACHY09_001296"
     CDS             7888..8238
                     /locus_tag="ACHY09_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884292.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTW
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            8254..8586
                     /locus_tag="ACHY09_001297"
     CDS             8254..8586
                     /locus_tag="ACHY09_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884293.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            8579..8776
                     /locus_tag="ACHY09_001298"
     CDS             8579..8776
                     /locus_tag="ACHY09_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884294.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            8766..9062
                     /locus_tag="ACHY09_001299"
     CDS             8766..9062
                     /locus_tag="ACHY09_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884295.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            9059..9568
                     /locus_tag="ACHY09_001300"
     CDS             9059..9568
                     /locus_tag="ACHY09_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFS3884296.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMLRQGFITDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            9638..10063
                     /locus_tag="ACHY09_001301"
     CDS             9638..10063
                     /locus_tag="ACHY09_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFS3884297.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            10135..10635
                     /locus_tag="ACHY09_001302"
     CDS             10135..10635
                     /locus_tag="ACHY09_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS3884298.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            10670..11098
                     /locus_tag="ACHY09_001303"
     CDS             10670..11098
                     /locus_tag="ACHY09_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884299.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            11082..11300
                     /locus_tag="ACHY09_001304"
     CDS             11082..11300
                     /locus_tag="ACHY09_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884300.1"
                     /translation="MPIPAEWLADCLVPPVPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            11311..11538
                     /locus_tag="ACHY09_001305"
     CDS             11311..11538
                     /locus_tag="ACHY09_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFS3884301.1"
                     /translation="MDDSDRALAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            11519..11830
                     /locus_tag="ACHY09_001306"
     CDS             11519..11830
                     /locus_tag="ACHY09_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFS3884302.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTN
                     E"
     gene            11823..12113
                     /locus_tag="ACHY09_001307"
     CDS             11823..12113
                     /locus_tag="ACHY09_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS3884303.1"
                     /translation="MSNFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            12116..12697
                     /locus_tag="ACHY09_001308"
     CDS             12116..12697
                     /locus_tag="ACHY09_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFS3884304.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            12697..14361
                     /locus_tag="ACHY09_001309"
     CDS             12697..14361
                     /locus_tag="ACHY09_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884305.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYCEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            14361..15950
                     /locus_tag="ACHY09_001310"
     CDS             14361..15950
                     /locus_tag="ACHY09_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFS3884306.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            15934..17265
                     /locus_tag="ACHY09_001311"
     CDS             15934..17265
                     /locus_tag="ACHY09_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFS3884307.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETNADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLARPKAVLWDKRHNNLMYIVENQDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            complement(17267..17346)
                     /locus_tag="ACHY09_001312"
     tRNA            complement(17267..17346)
                     /locus_tag="ACHY09_001312"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            17387..17860
                     /locus_tag="ACHY09_001313"
     CDS             17387..17860
                     /locus_tag="ACHY09_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFS3884308.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLTRSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            18037..19161
                     /locus_tag="ACHY09_001314"
     CDS             18037..19161
                     /locus_tag="ACHY09_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFS3884309.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTGLQTD
                     TVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            19161..20108
                     /locus_tag="ACHY09_001315"
     CDS             19161..20108
                     /locus_tag="ACHY09_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFS3884310.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            20152..20526
                     /locus_tag="ACHY09_001316"
     CDS             20152..20526
                     /locus_tag="ACHY09_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFS3884311.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEAEPCLTVRILQTSADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAGQDAPWKKTGNKAKQARA"
     gene            20523..20942
                     /locus_tag="ACHY09_001317"
     CDS             20523..20942
                     /locus_tag="ACHY09_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFS3884312.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            20939..21499
                     /locus_tag="ACHY09_001318"
     CDS             20939..21499
                     /locus_tag="ACHY09_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFS3884313.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            21500..21745
                     /locus_tag="ACHY09_001319"
     CDS             21500..21745
                     /locus_tag="ACHY09_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFS3884314.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            21742..23241
                     /locus_tag="ACHY09_001320"
     CDS             21742..23241
                     /locus_tag="ACHY09_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFS3884315.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATEFWGRGSMIALMVAEFIAINPDAELYAIAQGSGT
                     GQAQACLMNIIGTASQDGVLHIYIGGRRYLLAVTKGQKGKELIDALVSVINADADAPF
                     SAYAGQVADNASGLKGSMGINARFTGECSVHDIRVNYHDGETTPGGLTIELGYPKTKA
                     TNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWMAHTGT
                     FGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALPS
                     RMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLDV
                     ETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWVE
                     LGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            23250..23615
                     /locus_tag="ACHY09_001321"
     CDS             23250..23615
                     /locus_tag="ACHY09_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFS3884316.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            23630..24106
                     /locus_tag="ACHY09_001322"
     CDS             23630..24106
                     /locus_tag="ACHY09_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFS3884317.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            24233..26308
                     /locus_tag="ACHY09_001323"
     CDS             24233..26308
                     /locus_tag="ACHY09_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS3884318.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPSSGGAGFWGRMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGAL
                     WAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNY
                     LGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNV
                     WNGGNYGLW"
     gene            26295..27644
                     /locus_tag="ACHY09_001324"
     CDS             26295..27644
                     /locus_tag="ACHY09_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFS3884319.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIDRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            27628..28752
                     /locus_tag="ACHY09_001325"
     CDS             27628..28752
                     /locus_tag="ACHY09_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFS3884320.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            28742..29356
                     /locus_tag="ACHY09_001326"
     CDS             28742..29356
                     /locus_tag="ACHY09_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFS3884321.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNITVDKNLHVKGNV
                     SIDGTGRSQGAFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            29349..29786
                     /locus_tag="ACHY09_001327"
     CDS             29349..29786
                     /locus_tag="ACHY09_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFS3884322.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            29786..30868
                     /locus_tag="ACHY09_001328"
     CDS             29786..30868
                     /locus_tag="ACHY09_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFS3884323.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            30859..31419
                     /locus_tag="ACHY09_001329"
     CDS             30859..31419
                     /locus_tag="ACHY09_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFS3884324.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHKEENI"
     gene            31419..32330
                     /locus_tag="ACHY09_001330"
     CDS             31419..32330
                     /locus_tag="ACHY09_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3884325.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     DQNGADIQDKGTFIDNLGLRETVNKAADALPSAGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNTWRTVSSV"
     gene            complement(32365..>32860)
                     /locus_tag="ACHY09_001331"
     CDS             complement(32365..>32860)
                     /locus_tag="ACHY09_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS3884326.1"
                     /translation="YPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKLCISPVTGII
                     CSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKTVAEKRRSSL
                     LQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASEGHYQAIPWP
                     EKPE"
BASE COUNT         7737 a         8231 c         9689 g         7203 t
ORIGIN      
        1 agaaaaagct atttttgctt tacatggact acatttggat caatacttgt atcaaacttg
       61 ataacaaaat ttagttatca aatttcgtaa ctttttagga tagatgaatc atggcgagaa
      121 atgaagttaa gcctgattgg catcctcaaa gaatcttggg ggaagtcaga gccagagaag
      181 gaagtatgag ggctctggcc cgtaaagctg gtgtaggtca gaacaacctg tacaacgcgg
      241 tataccgtca tgttcccaag tatgagggga ttattgcgga atgcctgggt gtgccacccg
      301 aggagatttg gcccagccgc tacattcgta aggtcgcgta attatgtttg cttcagttaa
      361 agagctggtt ggattaccgg gaatgccggg aacggagcaa ggcgtccgct attcaatcag
      421 gaagtatgca accagcgaaa ccagcagacg ccgccaggaa ggcagtaaag gttttgaata
      481 cagcatcgac tgtttacctg aagtgacgca gcaggcatta cgtgaacgtt atgccctgca
      541 actgatgacg caaaaagccg atgaatcacc ggctccggtg gtgacaaagg ccagacgctc
      601 atctgacgtg gttgatgcgg tggaggcata tcgcggatca ccacaactga tggtcgaacg
      661 cctcaatgcc ctgactgaaa accagcgcca ggtggctgat gcacgaatcg cgatcgtcag
      721 cgaagtgatg aaagtcgcgc aacaacccgg tttcagctgc gcgaaggcta tccggtttat
      781 cgttgacaac ctggcacgtt cacagctgga cgagcgcatt gtggcaatgg ttgagacggc
      841 gaacgccaaa aagggaaaca gccgcgcgtt gagtgaaatc acgctgaagc gatggattgc
      901 ggcctttaac aaggcacaga acgccgctga acgcctgctt ttactggcac cgggtaaacg
      961 ccaggaaata aaagccgaag atattaactg gctgcccgaa tttctggcgc agtatcgcca
     1021 gtcaaacggc cgaccaatga ccgaggctta cgaggatttt gttgctgaat ggcggctccg
     1081 gcacgctgat gagccttata tgctcgatat catgccctct tatgacacca ttcgccgcgc
     1141 aatgaagaaa ctgccggaag tggtgaaaca aaaaggccgg gtgaccggca gtgaataccg
     1201 tcagcttgag ggattcacgc gccgcgactg gtccagaatg ccggtgaatt atgtctggat
     1261 tggtgacggt cacggcatga agatgaaatg caggcacccg gttcacgggc ggccatttgc
     1321 accggaagtg acctttgtta tcgacggtgg cacgcgcttt gtggtgggct ggagcatgga
     1381 cctggctgaa aatgttttcg ccgtagccgg tgccatacag cacggtattc gccatcacgg
     1441 caaaccgttt ctgtattact cggataacgg ctcgggggaa accgcagaca tcctggataa
     1501 ggaggttgtg gggatactgc cgcgactggg gattaatcac ccgaccggga ttgccggtaa
     1561 tccgcaggga cgaggcatta tcgaacggct taaccgcaca ttaccgatgc gcatagcccg
     1621 taaataccgc acctatttcg ggaaaggtgc agatcgcgag acgttacgca aaaccaaccg
     1681 cgatttacgc tcggcattta ctgccctgca acagggcaaa cggctgaacg cccggcagca
     1741 gtcagcgatg cgtgatttac cgtcctgggc tgaactgatt gatgccattc gtgatggtgt
     1801 tgagtggtac aacaaccggc cgcacgatga attacccata aaaccgaacg gcaagcatta
     1861 cagcccggcg gagttcagaa aaaaacgcct ggcggaagag gacacggaaa ttgaatggct
     1921 gtccgatgtt gagctgcggg atatgttccg gccgatggtg gaacgccctg taagacgctg
     1981 tgaaatacgc tggctgaata atatttacta cgctcccgag ctgcgtgatg agcatggccg
     2041 caaggtgctt atcagctatg acattcatga tgccgaacga attaccgtac gtcgcctgga
     2101 tggcagcgtg atttgcgagg cggtatgggg cggcaataaa cgcgaagcct tcccggtcag
     2161 cgcggaatac tacaaacagc agcaacgcct taaaggcatg cgtaaacgcg cagaggaaaa
     2221 aatccgtgat gccgaggatg aggtggtcaa cgtgctggag cacaagcagc aggagccatg
     2281 gctggaaaac atctatcgcc ctgtgggtaa tgcggtgatc gttcagcaac cggtcgctga
     2341 cgatgaacct gatgaagaat acgagcgtaa tttccagcgg ggattgcagt tgctggaagc
     2401 gaaattaaaa gaaaatgacc cgctggcctg aaataaaaaa ataacccgag cggcgactca
     2461 ggttatttga ttaaacaagg tatcaaatga gaggttaata atatgactga tattaacgat
     2521 gtaatcaaga ccattgatga acttattgat ggcggcgtac tgacgcagta tgccatcgcc
     2581 agagaggcgg gaatttccga cggcacatta tcggctttcc gtaagggaaa atataaaggc
     2641 gataacgctg ctgtggctgc ttccctgcgt tcctggtatg agaactggaa taaacaaagc
     2701 gcactgccgg aaccgccgca gtttgtggaa acgcagacag tccaggagct gcgcgcactg
     2761 tttcaggcgg ttcgtctgat gggctgtatt aacgttattg tgggcgtacc gggtgtgggt
     2821 aaaacggcca ctgcccgtaa ttactgccag gagcaaccaa acacctggat gatcaccctg
     2881 tcacccgcgc actccagcgt cacggagtgt ctgctggagc tggccgatgc gctggggatt
     2941 gattacaccc gcgcgaacaa aggggcatta tcccgcgcca tccgccgtcg cctgatggga
     3001 acgcgtggac tggtgattgt ggatgaggcg gatcatcttg gtattgacgg tctggagcaa
     3061 ctccgggcaa ttcaggacgc cacggggatc gggatggtgc ttattggtaa cccgcgcgga
     3121 ttgtttaaag gtggacgccg cgcctttgat gatttatcgc gcctgttcag ccgtcttgcc
     3181 cgtacaaaac aacttcgcaa ggccaaaaag gcggatgtgc tggccattgc cagggcatgg
     3241 ggtatcagtg gtgaggccga gctggccgtc atgcaggcta tcgctgaaaa gccgggagcg
     3301 ttacgcgttc tgacacatac gcttaaccag gcgtggctca ccgccagcgg tgaaggtgcg
     3361 gcgctgacag aaaaacatat taatgcggcc tttaaagagg tttataccaa ccctgaatta
     3421 ctctcacagg tgtgattatg gcggtattta atattcctga tatttacgga cgcttttacc
     3481 tggttaattt cgataacgtg aaggtgattt cactggccga aaataaagaa tgtggcgatt
     3541 tactttttga atttaatgac cgcacacgaa tggtgatatc tgccggactt gatcgcgaag
     3601 gtgcgacaga cgtttacagc ggaatatgcc gttctgttgg tgcgaaacaa gtcagctaaa
     3661 tgaggtgtta tatgaatatg caatcctgcg gtaacaaaat gaatttattc gactccctga
     3721 acagcgcgcg ccgtctgacc gaacttgccg gtgcggtact ggaacgcagt aagcgctacc
     3781 cacaacgttt tgcactgaaa accacgccgc cggtaggcaa cgtgcaggga accggtgaaa
     3841 ttgaaatcac catacagacc aacggcctgc gccgccgtgt gaaggccacc cgcatcagcg
     3901 gctgcacggt ttactgggag gtgtgaggtg aaaaaaaatc tcattgcatg ggcgtggtcg
     3961 agtggtctta ttgagtttgg ttacgtcctg ccggaaggtg cattgccgat agttgccgga
     4021 aagcctgcca cggtacggca tgtgattgag gttatggcgc gtcatggacg tgatgaacag
     4081 gagcagttac tggttccggg gataccggaa gcggtgacgg aggaagaagc ctttaatgcc
     4141 atgattcggt tctgccgtga ggtcagacgc cgggtcagtt atccaaacag aacgaggacc
     4201 agaggatgag taaagtcgta cgcattattt tcgaatacaa ggagcacgtt atccataaaa
     4261 acgcggatgg aacagtgcgc atggggataa gtctggacat acgttcaacc gggataaagc
     4321 agaaaggtga tggacctgcc atgatttttg gggtggttat gctcgcggaa agcagagact
     4381 ttgctgaact tgtggcaatg aaagccagtg cgctcatgaa agatatgagc atgcgttccg
     4441 gggttattaa aggtaatgaa tttaatcagc aggggtaatt ccatgagcaa agtacgcgtt
     4501 atttttgaat ttaagcatgt ttcgcatgac gaaaaaccgg caggcaatga ctgtgttgaa
     4561 gtgcatgaaa agattggtgt ggatgtaaaa acagaacgtg atacgaataa caggccgacg
     4621 tcactctgtg acgtttatgt aagtattctc cagtatcaca gtcctgaaat tattcagttt
     4681 ctctcagcgg aatttcaggc atctgtacag gcttttgggg cggatgccat cattaaacgc
     4741 caccgcgtgc ataaagcatc aggcacactg caataaggaa aaacaaaatg gcaaaacgcg
     4801 ttacaaaatt aaaggccgca gcagaggcgg caccgcagac ccgtgaagag gtcagccgcg
     4861 atatccgcac cctgggcgat attcagcgag aggcgctgcg cctggaaaca gcgatgaatg
     4921 atgaagtggc agaaatcacc gcccgttata cgccgcagat tgaaaacctt aaaaaagaaa
     4981 tcaaagtgct ttttaagggg attcaggact ggtgcaaaac caaccgtgat gagctgacga
     5041 acggcggcaa aaccaaaact gccaatctga ccaccggaac ggtgtcatgg cgactgggga
     5101 atccatcatg cagcgtcagt cgtgatgtgg aaggtgtgat tgaaatgctg cgccgtatgg
     5161 gccttgagcg ttttatccgc acgaaagagg aagtgaacaa ggaagcggtc ctggcggagc
     5221 cggatgcggt gaaagggatt gccggtatta aggtgaataa aggcgctgaa agtttttatg
     5281 tcgagccttt tgaacaggac gccggactga ataaataaca ccgcattaat caattaaata
     5341 tcacttcgtt ttaattatgg cgctcgcgtc aggggactgc ctgcgcctgt aaactgaaaa
     5401 taaggaacag gatatatggc atatttttat ttcaaattag accgtgtgca gacaaataaa
     5461 tatttcacca aatatcaaca gactgtttta ccgctacgca acagtattct tcgggcatta
     5521 ctgaaaaata caggcgctgc cggattgcgc ttaaagccgt ttgccatgga cgtaatcagt
     5581 gagttttatt tttctggtgc tctgcctgcg ggctggcgta agcgcgatga tgtggctttt
     5641 atcggggacg gaccgtgctt tattgccaga cctgatgagt catgccctga aggtccggcg
     5701 attgccgcaa tgattgaaac cgctgaacgt gagttaagaa agcgtcctga tttccttgtc
     5761 tggctctgtg aaaagctggg ggtaatgaga atcccctcca tgtttaacac ggactcctgg
     5821 tggaccccgt cgctctcccg tgatgccctg tgcgtggtgt ttaaagtagg cgcttatggc
     5881 agggaaataa aagggtgtat tcctgaagaa tgccaggaaa ttaaacattc tgaatatgtg
     5941 gcgctgacgg aggaataatt catgattgat gcaaaagtgc ttgaaggggt taaaaactgg
     6001 ctgagtattt acgggcgtct gacctgcggc attctggctg aaaaaatgaa tatgccgcca
     6061 tcctcgatgg tttattttct gcgtgatgcg gtcgatgccg gggtgctgac ggaatgtaac
     6121 ggtttttatg atattccgcg tccccgcccg gtgcagccgg ttcgtcgcaa atgcagccag
     6181 gaaggtgcgg ctgatgatgt tcagtggtgc agcttcagaa agtccctgcc gtggattgaa
     6241 gggcatgata ttccgtcgat ggcgtgggaa tttgctcagg gcgtgctgac ctgtgaaacc
     6301 gtttatgtgg tggctgaagt tgatgagcag gccatgaaag aaggcgtgcc ccagtttgtg
     6361 atggcgtata tcgacatccg cctgggcgtc attatctgcg gtttaagcgg ctggaatatc
     6421 acctcgcgcg ttctgcgtta cctgattgtt gaccggacgg ccgcgcctgc cgggatatca
     6481 gcggaggtgg cgtaatgttc tttaaaacat caaacccttc cgcgctggct gcgtggcaaa
     6541 aataccagca ggactgccag acggtaaaag atgaggcgaa acgccttgag gccgtgctga
     6601 atgttgcgtg ccggtcggta tttgaattca gtatcagtgg tttttgtttt aagggactgc
     6661 gttttacgga ggacaaatat ccttttcatc gtgatttatg gcgaaaacca actgcgtcga
     6721 atggctggag ctgcacaccg cgcacatcac gtattcccaa agccctgcgc gttgcctctg
     6781 acgaacttaa cagtctgtgg cgtgaatatt cgcccgtcac gtatgccaga accgatgcac
     6841 tgttgttctg gctgggcatt gatttctcgg caatcctgtt tggccctgtg gagtggttct
     6901 gcgttgacga tgtgatttac cttcagtgcg gcgtaaaacc cgcaaaacag aaaatgaccg
     6961 aaattctgtc tgatgagttt tatgctgctg aaaagcgagt cagggggtga tgcatgatga
     7021 tattacaacc catggggaga aaaggccgtg cacccgctca tgttcgcgca tggacacctg
     7081 aagaagatgc gctgctgatt gcgctttatc catccacccc ggttaaggat attgctgtca
     7141 ggataaaaag aagtttctgg ggtgtacata accggattgt tttattacgc ggtacttacc
     7201 cggagttgct caaatgcaaa cgcctcagat ttaaacctga tgaagataaa tttatccgaa
     7261 aaaatgccag aacaatgacc ggtaagcaaa taggagaata tctggggcga gaccgggatt
     7321 ctgtccacaa tcgggcgcga tatattggcg taagcatgaa aaaatacgga gagttgctgc
     7381 ccttcacccg catacctgac gatgatgttc gtcttattcg tgaattacgg gatgctgaat
     7441 caccacggcg tcttaccttt agggaaatcg gcgagaaatt tgaattatcc gagggcacgg
     7501 tgagttttat ttatcaccgt cgtcggactg ccgaagatgt tgtattacgg gaattaatgc
     7561 catgataacg acattatttg ttgaatcaga tgaacccctt gtgtgtgccg tcggaatgcc
     7621 ggtctgtggc ggaacgctga ccggtgttta tttcggggat ttgcgcggtt atccctggca
     7681 ttcactgaat gatgctttcc cgcctgatat ggaggctgtc gtgctgattg ttcagtatgg
     7741 tgaccaacag gaactgcgca tcggccatat ggggtatgaa ggctttttta ttgatgaaga
     7801 aaccggagcc tgccttgaag atgaggacgg acaggtgacg cactggtgcc atatttcagc
     7861 tttaccggaa tgacgggagg tgaatgcgtg agaagttata ttgataatga aaaactgaag
     7921 acaatcagcg actgtcttag cttgctggca aaaataaaag aaaccattga agagattaaa
     7981 tttcagcttg aatacgaacc ctgtggcgat gatacatggc gtaactctgc cagaaaagca
     8041 ctggctgtct ttcagaaaca acgccggact gttgaatacc gccttgctgt attgcgccag
     8101 gaggaaaagg aaagaaatat aagacgtcat gaactcgtta atgattttct tgttcgtgaa
     8161 ttaaaggagc gtgtgccgga gtcggtattt tttgagtgtg aggctgttgc ccgtagcaaa
     8221 gcactggaaa ctgattaaac aggcaggtga atgatgagta ttgaattaag atcgtcttat
     8281 gaatatcgta aaattctcat cgccggaggc atgaaaccgg aagatgcaga aaaaatcgtt
     8341 tcttttatgg ataaagaatg tgacaaacgg gatatgccag aaattattat ggatgacatg
     8401 attctgcatt cagccgtagc gttaagcccg ttatggattg tgcattctct tgctgaaatt
     8461 gccaaaggca ccgataaaca ggcagctgtt gctgcccttc agacgcttaa tgaaatgcgt
     8521 atatctccac gcccgacatt aatacatatg attctgtcca gcatggagga taaagcaaat
     8581 gaatagtcta cccgctggat gggcgcggcc actaatggcc aggaagcatc atttttttaa
     8641 aacaggcgaa aatatcagta tatgtggacg gtggttatat ctggctcata atcgcgagcc
     8701 ggatacattt gaaagccctg atgactgtgc cgaatgccgc agaagagtga ataaggagaa
     8761 agataatgga caatagttct ctgttgttct ggtgcttata tatcacatcc tttttcggcg
     8821 catttgttat tacccggtgg ttatgtcgaa aaatcatctg cttttttgat aaaagacatc
     8881 cggttgaacg ggcggctgat gcgctcattc agcaggccat tgtgttatat agcggtgaat
     8941 ttttctgccg gataacaacc agagatggct ggcacataat gattattcca ccaacacacc
     9001 atgcccggtg ggatgaggca gaaaaggctt tccatgtccg taaaaaggta aatacggtat
     9061 gagaggaaaa ctgatatccg ccattcatgt ggcaaagcgc gagcttgccc tggatgatga
     9121 gacttataca tccgcgctgc tggcagccac cggcaaaacc agctgccggg atatgtcacc
     9181 ggatgagtta tcccgcgtgc tggatgtttt caaaaaacgc ggttttaaag tgcgtcagaa
     9241 cccggttaac cgggccttaa aaccgggtac ggtgaccgcc aaaattcgcg ccatctggaa
     9301 ggtgatgctc cggcagggct ttatcaccga tggtgcggaa accgccctta accgctgggt
     9361 gaaatcgcag acggccgcgc agaacggcgg cgaaggtgtg gcaaactggc agtggctgga
     9421 gcaacacccc gccctggcct cagatgtgct ggagcgtctc aagcgatggc accgccgcaa
     9481 aatgctggcc gcgatgggaa tgcccgaacg cacgctgatg gggtatgacg ccgtttgcag
     9541 gcagtatgaa aaatcacttc cccgttaacc ccaaatcccg ccacaacgcg ggatttttat
     9601 tttaaactta ccgggaacgt gagaaccgga ggctgatatg gcagaaactc agatgagcat
     9661 gtttggtggt gacagtgagc aactgcacgc ccttatcgac cgccttgatg acatccctga
     9721 tgatgttctc aaaaagaact ggccgcggac cctgtcagaa ctggttgaag tcaccggcgc
     9781 agaacttcag cgtcagggga ttgagccggt actggccggt aaactggcac gcaaggtggc
     9841 tgcggctcag gcagcctata tgggcggacg gggttattac ctgccggtcg gggaatctct
     9901 ctttgccgag ctgcgaaaca atgagatatt ttcgcgctgg gaccggggcg agaaaattga
     9961 atcccttcgc cgccattacc ggatgtcaga aacccagatt tataccgtca tacgtgaaca
    10021 gcgccgtctg catctggcaa gaacgcaacc gccacttttc tgatattccg caaagcctgc
    10081 cgcctttcct tcaccgttac gctgactcag agaacatcat gagacagcgt aacaatgcca
    10141 aaactccccg caccactgcg taaaaagctg attgcccttg ttctggccgg tgccgggacg
    10201 ttcacgattg ccacgcatta caccggttac tgggaaggga aagaaaactc cacgtatatc
    10261 gatcccaccg gtacacccac catctgttac ggccataccg gcccggatgt gaaaccgggt
    10321 atgaccctga cggatgaaga atgcctggaa ctactggaaa aggacatgaa atgggccttt
    10381 gcggccattg atcggcgtgt tcaggtgccg cttacccgtg gtcagactgt ggcgctggct
    10441 tcgtggatct tctgggccgg tgaaacgaac ttccgcaact ccacgcttct gcgcctgatc
    10501 aatgccgggc agatgcccgc gtcctgtaag cagtatatcc gctggattta ttcaaagggg
    10561 gtgaaactcc ccggccttga ggcccgccgt tcggcggatg aatggttatg tcgctacgac
    10621 ttgccgaaag tctgaaccgc ttctggcgac cgctcatgat agcgctgctg tgtgcggtac
    10681 tgctgttacg gggtgtcctg tggctgcggt gaataatgcc ttaccggccc tgattgccgg
    10741 ggcgtgcatg gcggcgctgg ggattatcgc tgtactggtt gccgcgatat ggggaatgca
    10801 ccagaagacg cagcgccttg aagacaataa tcaggtgctt gtgcgtgagc gggatgaggc
    10861 gcgtcaggtg ctggcaaatc agcagcacac cctgcaactc atttcacaaa tcagtgaggc
    10921 ggccacgaat gaaaaacaac agaacattca gcacagcgag ggacagcaga gcgttgtccg
    10981 ccggtcgctg gcagcggtgc ctgcggcctc tgcccctgtt cctgatgatg tggctgatcg
    11041 ggtgcgccgg gccgtctgtg aaatacgtgc ctgtgaagcc ggtgccgatc cccgctgaat
    11101 ggctggctga ctgcctggtc cctcctgtgc cggagccgtt cacgtttggg gcatcggtca
    11161 cttacaacct gcaactgctg gcggtgatca agaactgcaa cgtggacaag gccagtattc
    11221 gtcgtctgga ggcgcggcga caacatgaat ttactgatat ggccggaacg cctgctgttc
    11281 cggcaggaaa gacgaagtaa ggaaaaaggt atggatgatt cagatcgtgc tctggctgtg
    11341 atggagcggg gatcagaacg cgccctttgt aaccggctga cacgtaagcg ccgggcaatg
    11401 gatgctccgg gcagacgtgt ctgcgcggac tgtggcggag agatccccgc cgcacgtctt
    11461 gttgctgtgc cggatgccat ccgttgtgtg aactgccaga acatcatgga ggcccgtcat
    11521 gtgggtcagc atcgttaaag attatgttgt gccgatcctt tcggcgacgg caaccgctgg
    11581 cgggatattc atggcgctga tgcgcaaaac gtttgtcccc cgcgaggcct ttgaaaaact
    11641 ctccgaccgc gttgagaagg tggaaacccg cctgtcgtca ctgccgacag aagctgaggt
    11701 caaccgtctg aatgtggaaa tcgtgaccct gcggggtgaa ctgaaaacca cgaacgccac
    11761 gctccgctct gtctcctatc agaacgaact gctgctggag caggcggtaa ggaaaaaaac
    11821 gaatgagtaa tttcattact gaagatcagc gtctggtcat tctgcggtcg ctggcagatt
    11881 acaacggtga actgggtgaa tccgtgctcc aggactgtct ggatgattac ggccaccgcg
    11941 tgtcccgcga caccgttcac acccacattg cctggcttgc cgagcaggga ctggtgcgca
    12001 aacgcgttct gattaacggt tatttcatcg cggaactgac cggccggggg caggatgtgg
    12061 cggaaggccg cgccagcgtt ccgggtgtga agaaaccacg cgcaaggggg taacgatgga
    12121 caagccgacg cgtggacgcg tgcgcaaggt ggatttgctc cccgacagca tccgtaagcc
    12181 actgctggaa atgctgcgtg aaaaacgtct gacgcaggtc cagatacgcg aggaaatcaa
    12241 ccgtctgatc cgtgaggcgg gcctgccgga agagcagcag ctttcaccgg cggccatcag
    12301 ccgggaagcc tcccgtaatg agctgattgc ccgtaacctg cgtgatttac gcgagcagac
    12361 aaaagccatg atggccgaac tgggggacaa gccaaccggt gaaaccactg cattgatcct
    12421 ggagatgtcc cgcgccctga tgtaccgccg tctgcgtgcc gcgaccgaat ccctgaacag
    12481 tgacagcgac gtggatatgc gtcttattaa ggacatcctc ctgtcagcac agcgtgcgga
    12541 aagtgccgcc gagcgcagca ttaagcgtga aaaagaaatc cgggccgcat ttgctgaaga
    12601 gatggcaaac gcggtcaccg acgagctgcg cggcgtggac gggatgagtg aacagcttga
    12661 gtcccgtatt aaggggattc tgttgggtaa agcctgatga ataacgccga agaactctcc
    12721 cccttactga cgaataccgt ctccacccgc aaaattgatc tggccggtga aaaagccctg
    12781 ctgggcgtgg atgtgccgga cagccttgat ttgcccggtg atatgccggt atttctggat
    12841 tatcaggccc gctggtttga ggatgaaagc gaggtctgca tcgcggaaaa atcccgccgc
    12901 accgggctta cctgggcgga agccgggcgt aacgtcatta ccgccgcaaa accaaagcgg
    12961 cgcggtggcc gtaacgtgtt ttatgtgggg tcaaaacagg aaatggcgct ggaatatatt
    13021 tctgcctgcg ccctgttctc ccgtgccttt aaccagctgg cagatgctga tgtgtatgag
    13081 cagaccttct gggatcggga taaaaaagaa gaaattctga cctacatgat ccgctttccg
    13141 aacagtggat tcaaaattca ggcactgtct tcccgtccgt caaacctgcg cggccttcag
    13201 ggggatgtgg tgattgatga agccgcgttc cacgagtcac tggatgaact tctcaaggcg
    13261 gcaatggcgc ttaccatgtg gggcgcgcgc gtgcgtatta tctccacgca taacggcgtc
    13321 gataatctgt ttaatcagta cattcaggaa gcccgcgagg ggcgcaagga ttacagcgtc
    13381 caccgcatta ccctggatga tgccattgcg gacgggctgt accgtcgtat ctgttatgtc
    13441 accggccgcg aatggtcacc ggaaagcgag cagaagtggc gtgatgatct ctacaaaaac
    13501 gccccgaccc gtgaggatgc cgacgaggaa tacggctgta tcccgaaaaa atccggcggt
    13561 gcctatattc ctcacgcgct tattgaaatg gcgatgatcc gcgacatccc gattctgacg
    13621 tttgaagccc cggacgactt catcagccgt gccgcatggc tgcgtgaatc ggaggtttta
    13681 acctggtgtg aagaacattt aaaaccgctt ttagaggcgt taaatccccg ttcccgattc
    13741 agcttcgggg aagactttgc ccgcaccgga gacctgtcct gctttgtgct gctggaaatc
    13801 actgaatccc tggcaaaacg cgaggtgttt cgcgtggagc tgcgcaacct gccgtatgcc
    13861 cagcaggagc aggtgatgat gtacatcctg acccgcgttc cggcgctggt cggtgcggcg
    13921 ttcgacgcca ccggtaacgg cggctatctg gccgaagccg cgctgctggc ctttggcccg
    13981 gacatcatcg actgcgtgat gttatcgccg aagtggtact gcgagtggat gccaaaactg
    14041 aaggccgagt ttgaggatca gaatatcctc gtcgcccgcc atcagaccac gcttgatgat
    14101 ttgcgccatg tgaaggtggt gaacggtatc ccgcaaattg acaaggggcg tacgaaggac
    14161 cagaacgcaa cggctgtgaa cgcccgccgc catggcgatt ttgccgtggc gctctgtatg
    14221 gcaaaccggg cgtcatacat ggagggcttc atcctggatg aatcagcctg tcaggcgctg
    14281 ccggagcggt cgcgggccat ggagggcggt tatcgtgatg acgatgaggc atatcatgaa
    14341 tttgatcggg ggtgctggta gtgggacgta taattgatct tgacggaaaa cctttctcct
    14401 ttgacccgga gatgcagagt gccgcgctgg atattccgca gattgccagc cgttatattg
    14461 aacatccggc ctcgggtatc accccgaacc gggcggcaca gtgccttcgc ggggctgaac
    14521 gtggcgatct gattgcccag tccgatctgg ccgctgacat tgaagaaaag gatacccatc
    14581 tttttgcaga gctgggcaag cgacgtcttg ccattcaggg ggtgccctgg agtattgagc
    14641 cgcccccgaa cgccagcgcg aatgagaaaa aggacgcgga aatgctcgac gaatacctgc
    14701 attccgccga ctggtttgat gccatgctgt ttgatgccac cgatgccatc ctgaaaggct
    14761 attcctgcat ggagattgag cacgggatgc tcggtaaaat gcacatcatc cgcgccatcc
    14821 gctggcgtga cagcgggcat ttctgcctta acccggatga tttgagcgaa ctgcggctgc
    14881 gtgacggcag ccatgccggg gtggcgtttc agccctttgg ctggatagtg catcagtcac
    14941 gttcacgcac cggttacggc ggcgcaacag ggcttgtcag aacgcttatc tggccgttca
    15001 ttttcaaaaa ctattccgtg cgcgatctgg ctgaatttct ggaggtgtac ggcctgccga
    15061 tgaaggtcgg taaatacccg tccggggcaa caccggagca gaaaagcgcc ctgatgcggg
    15121 cggtgatgga tatcgggcga cgtacaggcg ggatcatccc ggccgggatg tcgctggagt
    15181 ttcaggcagc cgcgaacggt caggccgatc cgtttgaaac catgatttcg tggggggagc
    15241 gttccatctc caaagcaata ctcggcggca cgctgaccac ggaagccgga gacaaaggcg
    15301 cgcgctcgct gggtgaagtg cataacgagg tgcgccggga aatcagggat tctgatttac
    15361 gccagctggc cgccacgctg aaccgcgatc tggtgtatcc gctgtatgcc ctgaacaccg
    15421 cccacgccat tgatatccgt cgcctgccgc gtatctgttt ccagacaaaa gagccggggg
    15481 atatcaccaa aatcaccagt gcggtgatgc agctcagtac ggggatggat attcctgatc
    15541 cctgggtacg ggagcagacc ggtattccac agcctgcccc cggtgaagcc atcttccgtg
    15601 tccgtcagag tggcaatgaa cctgctcaga cagacaaaga gatgccaccg gaaaaacagg
    15661 agaagacaga gcagacagcg ctggctgcca gactgccgga agcgaagagc agcccccgag
    15721 atgaactgga cgacatgggg gatgcggtgc ctgcccgccg gttacaggag gccatcgacc
    15781 cggtactgga gccggtcatt gaagccatca gaacgcgggg gctggcggag gcactggcag
    15841 acctgcctgc cctttaccgt gaaatggatg attcccgcct gatgacgctg ctcagtgatg
    15901 ccatgtttgc tgcggaaatg aaggggatgc tggatggcac aggggattga tttaggttat
    15961 gccgccacgc ttccctcaaa agaggcggtg gcatacttcc gcgccaaagg ggcgcatatc
    16021 agctggaact ggttcgaaac aaacgcagat gttcatgccc gttcattcac ggcggcaaaa
    16081 gtggcacgcc tggacgtact gaccacacta caggcggaag tgcagcgtgc tgttgatgag
    16141 gggatttcac agaaagcatt tatccgcaca ctgacacccc gcctgcaaaa gctgggatgg
    16201 tggggaaaac agattgtggt ggacagtgcc ggtaacgcgg aagaagtgca gctgggcagt
    16261 ccccgccgtc tggcgctgat ttacaacgtg aacacccgtg tggcttacaa tgccgggcgt
    16321 tacacgcaga tgatgaacaa cacggacacg catccgttct ggcagtatgt ggcggtgatg
    16381 gacagccgca cccgcccggc gcattccgcc cttaacggtc tggtattccg ctatgacgat
    16441 ccgttctgga atacacacta cccgcccaat ggctggaact gtcgctgccg tgtccggcca
    16501 ttgtctcagg cccgtctgga tgcgatgggg ttatccgttt catccggtca ggatcatctc
    16561 tccacccgca atgttgaggc tggcgtggat aagcagaccg gggaagtcag agaaatgccg
    16621 gtgaccacat attcagatgg caccagaacc atgacaccgg atgtgggctg gtcatataac
    16681 cccggttcgg cggcgttcgg cacagaccag gcgctgatcc gtaaactgat cgaggtgaaa
    16741 agcccggcgc tgcgggaaat ggtggttcag gagatgaaca acagcccgga gcggcaactg
    16801 gcgttccgca tctgggcaaa aaacatcatg aaaacccggc gaggcggtaa cgatatccgc
    16861 acgctgggct ttatgaccga aagcattgcg caggcggtgg aaagccggac gggaacgcca
    16921 ccggcccgcc tgctggcgat gagcggtaaa aatgtgctcc atgcggacag catgaaacat
    16981 cagaatgacg gtatcgccct gacgccggag gatttcgggc gcttaccggc aatgctggca
    17041 aggccaaaag cggtactgtg ggacaaacgg cacaacaatc tgatgtatat cgtggaaaat
    17101 caggatggca gtgtgcagat tgccgttaat gcaccgtaca gcctgaagcg tcagccggat
    17161 aaactggatg tgattgttaa tgcttaccgg gttatcaata tggataagct gaaatcggat
    17221 atccggggcg gaatgcttga ggtactggaa ggagatattg attaacggtc agtggcgggg
    17281 gtcgaacccg catacatgat ccacccggag gcaggcaccg actttaccag ttaagcgtac
    17341 actgaccgcc tgtttatttt atattcatcc tttatgcgga ggcaatatgt catccattga
    17401 tgccgctgtc gttgttgacg ttacgcgtct ccagcgggtc tttgcccggc ttcagtttgt
    17461 gggtggtgga aaagacctga cccgcagtgt ggcgtccagc cttctgtcat cgtcagaaat
    17521 ggcgtttgaa caggaaaaag agccggacgg cgaacgctgg catgactggt cagatcctta
    17581 ccgcaagtgg cgtacccgta agggatacat gcccggcaaa atcctgacgc tgaacggcga
    17641 tctggcccga cgcctgacca cggattatgg cgatacctgg gcgctgattg gatcaaatga
    17701 gccttatgcg gccattcatc agtggggggg cctgccaggt atgccgcctg gaccggcggc
    17761 cattggtgca cgtccgtata tgggctttga tcaggtggca gagcaggaga tcatggacga
    17821 aatcagaaaa cgctttaaaa aggccacaga aacgccttaa ttgtttaagg tatgcaaatg
    17881 cattacctta cccccttcag gcgcgtgtcg tgattttcta acctgaattt aacgggcttt
    17941 aaaatctgcg tgaagcgcct ttgtattttt cccctgaaag cacttcctga aatcccgcaa
    18001 aacccgccct gaaaattccc ggcctatgct gccggaatga agacgaaaaa cacgcccaaa
    18061 cttgcttatg ccattctgaa tgccatcagc ctgtccgcag acggggacgg tgactggtgt
    18121 cagatcatgc cagccggtcg ggtaaaggcc cgtgacggtc gcccggaaaa accggccgag
    18181 ggctggctga tcaaccatgc agccgttgag cgcatggtct cccgtgttgt ggcgctcaat
    18241 cagccggtga aaattgacta caaccaccag accctgattg agggacatcc ggcaccggct
    18301 gccggttttg tgatggcctc accggagaat tttcgcttca gtgaagagcg aggtttcgag
    18361 gtgcgcccga agtggaaccc tccggccctt gaacatctgc gtaataacga attcccctgg
    18421 ttttcaccgg tgattggcta tgacgagagc accggcgaac ctgtcgaact tcggatgctg
    18481 gctattaccg gtgaccccgg tctgaccggc atgaatcctg tcgccgcact gtcggcggat
    18541 gacctttata acgccttaaa ccctccttta aaggatacct ccatgaatga gcaattacgc
    18601 cagttgctga cggcgctcgg tctgaccgtg gctgacggtg acgaatttac gccggagctg
    18661 ggcacggcag cgctgtctgc cctcaccggg atcaaaaccc gtgcggatgc acacgacaac
    18721 ctgaaaacac aggtcgccag tctgtcggca gagctggaaa ctgcaaaagg cacaccggct
    18781 ggcggcagta ttgacctgac gaaatacgtg cccgttgaaa cgtataacgc cctgcgtacc
    18841 gaatacgttg cgctgtcggc gcagcacggc agcaccacgc tggagcagtt gctggacaag
    18901 gccgagtccg aaggacgcat cttcaaaagt gaacgcggct atctggaaca actgggcggg
    18961 cagattggcg tggcggcact ttctgcacag cttgacgccc gccagccggt tgcggccctg
    19021 accggccttc agaccgacac cgtgaccgtg ccggataaaa agactgccac cgctgtgctg
    19081 tcggctgaag atatcgcggc cgccaaactg ctgggtaaaa ccgaagccga gttcctgaaa
    19141 atgaaagagg aaatgcaata atgcctaccc cgattacacc ggcgatgatc accgccctga
    19201 tgacgggcta ccgttctgat tttcaggccg ggatgtccat ggccccgtcg cagtacaaaa
    19261 aaattgcgat gaccgtgccc tccacatcga aatccaacac ctacggctgg ctggggcagt
    19321 tcccgcagtt ccgtgagtgg atcggctccc gcgtcatcga gaagatgaaa gcctatggct
    19381 atgccatcgt gaacaaaact tttgaaggca ctgtcgccat tagccgcgat gacttcgaag
    19441 acgacaacct cggtatttat tccccgttgt tccaggagat ggggcgcgct gcggcggtac
    19501 aaccggatga gctggtcttt gctgccctgc gtgacgggat cagtgcggcc tgctatgacg
    19561 gtcagaactt ctttgatacc gaacatccgg tttaccccaa agtggatggc tccggggatg
    19621 cgcagatggt cagcaatatg tttgtggcaa aaaccggctc tgtcggcgca caggctgatt
    19681 acagcggtcc ggcctggtat ctgcttgact gctcccgcgc ggtaaaaccg ctgatttatc
    19741 aggatcgccg taaggctgaa ctggttgccc agaccaaagt cgatgaaggt cgcgcattca
    19801 ccgataacga atttgtgttt ggtgcatccg cccgtcgcaa tgtgggctac ggcttctggc
    19861 agatggccta catgatgcag tcaccgctga cgctggatgc actgtggcac ggctggtcag
    19921 ccatgcgcga atttaccgct gacggtggcc gcaaactcgg cattaaaccc acccatattg
    19981 ttgtccccac ctcgctggaa aaacaggcgg tgcagctgct ggagcgtgaa ctgttcgcag
    20041 acggaaacgc aaccgtctct aacgagatga agggcaagct ggagctggtt gtcgcggatt
    20101 atctgtaaac ggtgagccgg gcttgcggcc cggcccctgc ggagggcaaa tatgaatgaa
    20161 catcacactg tggcagcaac agatgattca ggtcttcagg tcagtggtga caattctgtc
    20221 acggtgctgg cagaagtgcg ttgcagtcgt tcggcgtttc gtcgtgcggg gtttctgttc
    20281 acgcgtggac gtcagcaggt tgaggtcacc ccggagcagc ttgcccgact ggaggcggag
    20341 ccatgcctca ctgtgcgaat actacagacg tctgctgatg atgcggggag cgtggcgggt
    20401 gtggttcatg cagtggccgg tacagattta gccgaagccg aagccgaagc cgaagccgaa
    20461 gccgaagccg gacaggatgc tccctggaaa aagaccggta acaaagcgaa acaggcccgt
    20521 gcatgaatta cgccactgaa accgatatgc gggcgcgtta ccgcgaggat ttgctcaggc
    20581 cgttactggc tgtgccccgt tcggatgagc cggatacgcg caagctgaac cgggcgctga
    20641 cagatgcgtc agcccttatc gacagctatc tgtccgcccg ttacacgctg ccgctggagg
    20701 ttattccggc cgttcttgtt cagcactgtt gcgcgattgc cttttattac ctgtgcgatc
    20761 agcgagcctc agatcaggcg cgtgaccgtt accgtgaggc gctggcctgg ctgaaagatg
    20821 tcatgaacgg caatgtgccg gtaggtgtgg atacgaacgg agcggcccct gaatccgggg
    20881 atttaccaca ggttcagtct gatgcagcgg tattcgggcg caaccagaag ggcttcatat
    20941 gattacggaa accgaacagg catacatcgc ccgtatccgt gagtatttcg ggaatgaact
    21001 ggtgtctgtt gacacgcatc ccggcgactg gagcgacagc gtactgcgca ccatgctgat
    21061 taacgccccg gcgatctacg ttgcctggct gggtgccggt gaaggccgta cccgtggtcg
    21121 cctggtcagt cactgggtgt tctacgtcat cggcgacatg ctcaacgggc gtgaggccag
    21181 ccgtcccgga ttgtatcaga ttgtggcccg gctgattgcc gtgcttaacg gcttcagaac
    21241 agaaaaaacc tcaccgcttt actttgaaaa ggcggtcaac ggttacaccg aaacccaggc
    21301 ggacagtggt gcggtgatgt atgcgcttta tttctcctgc gaggaaatga tcgcaccgct
    21361 gaccgatatc agctcgctgg acgacttcct gcgtcattac gaaacctttg tggagccgcc
    21421 cggtacgccg ccgtttgagg cacatattga tttgccgggg aacacgacaa cggcggtcga
    21481 accgccggag gaactgtaaa tgaaaaccat ctttatcaaa cccgcgccgg gacgcctgat
    21541 tcgtgatccc gacacaatgc gcccgctggc acaggagggg gaggaaaagc cctttacgcc
    21601 gttctggtgt cggcgtcttg atgacggcga tgtcattcag gctgaaaaag ccgctgaaga
    21661 ggctccggct gtatctgcgg atgcaacgac tgcgacagaa aaaccggtcg cccaaccggc
    21721 atccgataag gagaaaccgc aatgattaat tttgacggga tcggtaatga ccgccgtata
    21781 ccgctgattg aggttgagtt taataactcc atggctgtca ccggcactcc ggcacagcgt
    21841 cagtgtgtgc tgctgtttgg tcaggcgaga atgaaagaga atgccgtgga cggtgccggt
    21901 gttctggatg ttccggtcag gatcacccgt gcgtctcagg caacagaatt ctgggggcgc
    21961 ggctccatga ttgccctgat ggtggcggaa tttatcgcca tcaatcctga tgctgaactg
    22021 tatgccattg ctcaggggag cggcaccggt caggctcagg catgtctcat gaatataatt
    22081 ggcaccgcct ctcaggatgg tgttctgcac atttatatcg gcggtcggcg ttatctgctg
    22141 gctgtcacga aagggcagaa gggtaaggag cttattgacg ctctggtctc tgttattaat
    22201 gccgatgctg atgcgccctt cagtgcttat gcaggtcagg tagcagataa cgccagcgga
    22261 ctcaagggaa gtatgggaat taatgcccgt tttaccggtg aatgttccgt acatgatatc
    22321 cgtgttaatt atcatgacgg agaaaccaca ccgggtggac tgaccattga acttgggtat
    22381 cccaaaacaa aagccactaa cccggatatc acccgaagcg tggcgggtat gggcgatcgc
    22441 cagtacaact atgtcgtcat gccctataag gatgatgcaa acctgaaaat catcagtgat
    22501 gagcttctga aacgctgggg accggccaaa atgtctgacg gtgtgctgtg gatggcgcat
    22561 accggcacgt ttggtgaggt gcaggcattc ggggcaaaac gcaatgattt tctggcgacc
    22621 tgcacattca ttcccaaagc ccctgaaccg gattacctct gggcagcggc ggtctgcgcc
    22681 acctgtgcgc cgtctctggg ggcagatcct gcgcgtccgc ttcagacgct ggcgcttccg
    22741 tcacgcatgg ccccggcccc ggccgatcgt ctgacgcgtg aagagcgtaa cagtctgctg
    22801 tggggaggga ttgccacggt cagtgtggct gccggtgatg tggttcagat cgaacgtcag
    22861 gtgaccatgt atcgccagaa cgtgtacggc gaaagtgatc ccagctatct ggatgtggaa
    22921 accatctaca ccctctcctg gctgcgttat tccctgcgta cttttatcac acaacgtttt
    22981 ccgcgccata aactggctga tgacgggacg cccgttcgtg ccggtcagaa tatcgtgacg
    23041 ccggagatca tgaagcttca gttaatttcg ctgggtgaag agtgggtgga actcgggctg
    23101 gtcgaaaatc tggacacctt taaaaagaat ctgcttgtgg agcgtaatgc gtcagatcga
    23161 aaccgtcttg acgtgttatg cacgccggat ctggttaacc agttccgctt cctggcagca
    23221 cagatccgtt tcattttgtg aggtaaggca tgagcggaaa acagtatcag ggcacggcca
    23281 ctatccgtgt gaacggtcag gaatacgaga cgcttgaggg ggctacattt tctccgtcag
    23341 gttttgaacg tgaagtggtg aaaggcgcga aagtctatgg ttaccgccag aaaccacgtg
    23401 aagcgacact ggactgcaaa tttccggcag gcggagaagg ttcaccagcc gccgatgaaa
    23461 tcaacaactg gactgctgtc actatcgagt ttgtggcgga taccggcgaa gtccacatga
    23521 tgacgaaggc ctggagcagt gaaccggcct cgcttgacgg tggcggggag atctccgtga
    23581 agttcgccag cgccaccagt acccgtgttc agtgatcagg aaaaataaaa tgaccacacg
    23641 taagaaaaaa acggcagttt ccgaggcagc cgtgatggag gcaatccggg aagcacttga
    23701 gggggctgat ccacgcactg ccgggctgac agagcaactg gcaaagggat atgtggatct
    23761 gcttgatggt ctgccgtttg gtgagacccg tgaatatcgt gtcacgttcc gggaactgac
    23821 agcgaaagac agtattgacg cagaagcaga agccgagcgc gtggtggaga caaacaatgg
    23881 cccgatgctg atagcgtctc cgtcgttacg cggtgttgca ctgctgcgcc gtcagattgc
    23941 cgcagtggga gacattgaag gtccgttgtc accccgtcag attggacagt taagcgagcg
    24001 cgatctctcc cgcctgatgg cagcggtcag cctgctggat accgcgcttg ccggaaagct
    24061 ggcagctgac cgggggcgat caggcgcagt gtcgggatca gattgaagaa gcggcaatta
    24121 tcctggggat ggtgacaaaa agtggcccgg aatgggcgct taacctcccc ctgtcgcaac
    24181 tttaccggca ctgccgacag acagaaaaaa tcatcagaac gaagcagtaa gtatggcccg
    24241 aaatctcaga gcatctctga tagttgattt gctcggcaat atctcagcca aatcccgcca
    24301 gtggtctcag gagctggggg cattctcacg ctccggccag gcggggctgg gtggtctggg
    24361 aaatgcggcc cgccgtgccg ggcaggaaac agatgtactc ggaagcagga tgcagcgcac
    24421 gctggccggg gtgcggggca gtatccgcca tgtcacttct gattttgacc gtcttcaggg
    24481 cagtattacc ggcaccatcg ggcggatcag caatctttac ggaatgctgg ccggtggtgc
    24541 agctgtatat ggctttaacc ggggatttat tcgcccggca gcggagatgg aaacctatat
    24601 gattcgcctg aactcccttt acaaagggga tcgggcgaaa actgatgatg tccgcaggtg
    24661 ggcaatacag aatgcgaagg aaaccacctg ggggctggct ggcgtcatgc aggaatatac
    24721 gtccagtctg ggctttggta tgagtgacag ggaggcccga aattttgtca ccatgcttca
    24781 ggatcagggt gccgtgggcg gctggtcact gtccgatgca cagggggcat ccctgcaact
    24841 gaaacagatg tatgcaagag gcagtattca ggcagcggat gcaaatattc ttgccaccta
    24901 cggcattaat gcctaccgcg tgctggctga tcgtctgggt gtggaccaga aagtggtcag
    24961 aaaacttggt gaaaaagggc tgcttgggcc ggacagtatt cgcctgttat ttcagacgct
    25021 ggcggaacag gccaggggag cacaaaagga cgcaatgaat tcctggagtg gcctgacggc
    25081 catgatgggt gacgtctggg atcagtttgc ccgggatgtg atggacagtg ggccatttga
    25141 aaaactgaag ggtaatctca ggggatttct ttcatgggtt gacagtgcca aatctgatgg
    25201 cagttaccat tccctggcgg aaagcacggc atcagccatg aaccagggct ttgagtatgc
    25261 ccgtgatgca gtgacagggt tttatcaggc gatcagaaag gtacgcgaca cgcttcaggc
    25321 tctgcgggat gcgggctatg gtgatgcgct ggatcgcatt ggtcaggggg cacagaccgc
    25381 cgcgaaatac ctgctgtaca tgtatctcgc cacccgtgcc ctgaaaatgg cgcgggctgt
    25441 cggaacgggg attatacgtc cgggtgctgc attgctggga tacggattat cagcggcggc
    25501 cttcctgacc tcacctttcc ggcgctctcc tccggccgga acaccaccag gcacatctca
    25561 gggacgaggg caacgtttta taaactttct taccggggta aatccggcag ccgttcagcc
    25621 agtacttgta acaaactggc ccacaggagg actgtccggc accggcgcga ccgatacatc
    25681 ctcaggcgga cgtcaggggc ggggccgcag gaaacgcgga ccggggcgcg gaagaccggt
    25741 cacgcctccc ctgccaccgt ccccagtgcc accgtctccc tcttctggtg gggcagggtt
    25801 ctgggggcgc atgatggggc gcgccggtgg gttactttcg tctgtcggta gccgcatggg
    25861 gctggggcgt ttctccggct ttttccgcag tgccggtgga ctggccggcc ggctgggtgg
    25921 aggtgccctg tgggctggcg cgatggcagc cccggttctg ctggatggca gcgccagtgc
    25981 cacagacaag ggagaagcgg tcggctctct tgcaggcagt attgccggtg gtgcgctggg
    26041 ggcggcagcc ggacctgtgg ggattgccat tggttctacg gtgggcagct atctcggtaa
    26101 ctatctgggg ggatggctga ctgaagcctg gcagaaatta cggggcagtg atgatgaaag
    26161 cagtggacag gcggtacaaa aagcctctgc ccgtgtggag ctggttgccc cggaagggtg
    26221 gcaggctcgc agtattgata ttgatgacac gtccggtcac ggactggatg tgaatgtctg
    26281 gaacggaggg aattatggcc tctggtgacg gtcgtggtgc gttccgtggt gtgccgttcc
    26341 tcgtctggcg tgagcagcgc gaacgtggtg ggcgaaacat tgtccgtcgg gaatatccgt
    26401 tacgggaaac cggtggtgca gacgatctgg gacctaaact ggctgaattt acgttcagtg
    26461 tacttgtgat gggggatgat gttcagacac agcgaaatcg tctgcgtgat gcccttcgtg
    26521 ctcccggtgc cggggagctg cttcacccgg attacggcac gttaaatgtg ctgataaaca
    26581 gctttgaaag tcgttataac gccgctgaac agggcgtggt tgagttcacc attaatgtca
    26641 cgccggtaag tgatgatacc gcacctgctg tcacacagga cacggccgcc attctggagc
    26701 agaagagtac caccgcgctc gggcaggttt ttgaaaccct ggaagcgggc tggacggtga
    26761 tctctgacgg aatgcacgat gtccaggcaa tgaccgaaac catcagtgat aaggtgtccg
    26821 ccctggaaaa tgccgtttcc ggcatgggga ttgtacagga tatcagtgcc tttactgcca
    26881 cgttcacggc gctgaaaggg aatgccacgg cattactgac agcaccttcc cgcatggcct
    26941 catcttttgc ggggcttttc agtgccctga tcaccctgcc atcgctgccg tcactgtctt
    27001 caggaggcct gaatacccgg cccggtggca gtatcgaccg gacgacatcc gccgtctctc
    27061 agggaatgcc gcaactgtac aggacattat cttccctgcg ttacgtgctg gatgagcagg
    27121 ataatccgca gactctcatc ggtctgacac cggcagcaca aaagaatatt cgtctgatac
    27181 gggcggtaat gcagagtgct gccgtggtgg cccaggcaca gaccgtggga aaactgctgg
    27241 atcaggttct cagtcaggaa accctgccgg acagtgacgc agcccaccgt acctggcctg
    27301 tctggctgga aagttcagtt gatcttcagc gcattaaccg tgacttaagc gaagcgctcg
    27361 aacggcaggt gatgacactg tccgggcagg ggtataccgc cacggcgctg acgcttcgtg
    27421 atgccagact ggcactgaca gaggatctga atacacgggg agttcagcta cccggtgcaa
    27481 cagtagtgac tgtacgtacc accgagcctg cactggtgac cctgtaccgt gccaccggga
    27541 acagtaccgg ctggcaacgt tttgtgcgcc gtaacggtat tgttgatccg ctgttcattc
    27601 ccggaggcca ttcagtggag gtgattagtg agcagcaggg ttgaactgta tctgggcggt
    27661 gagatttttt ccggctggct gacggtgagt gttcgtcgct ctcttgaaca tctggcgggc
    27721 tcctttgaac tgggggtaat gatgcccggt gtacgccttc cgtcatccgt ccgtgccggt
    27781 cagtctctgg aattgcgcat tgacggtcag cctgtgatca ctggctggct ggatcaggtc
    27841 cggcagcgca tcagcgccac gcgttttcag atcacgctca gcggacggga taaaaccggt
    27901 gacctggtgg actgttcagc cattcatccg ggcagccagt ggaggaaccg cacgctggag
    27961 cacattgctt cagatttgtg tgctccgttc ggggtcacgg tgcgctggca ggtaaatgat
    28021 gcaacggcag cccggccctt ttccaccttc acactggaaa attcagaaac cgtggcagat
    28081 gcgctgacac gggccgcgag acacagaggc gtcctggtaa ccagtaatgc cgccggtgaa
    28141 ctggttttca ctcaggccgg cagtcagcgt ggcgacacgc tgacgctggg cgaaaatctg
    28201 ctggatttgg atcacaacgt ggatcaccgt ctgcgccaca gtgaataccg tgtacggggg
    28261 cacgggcgtg gtggtggtca tgccggggat gcactgacag ccggaacgct ggccgcacca
    28321 gttggtacgg tgacagacag tgccatccac cgttacagac cgaaaattgt gctggcggat
    28381 catgctgttg atgcagacgg tgcacgccag agggctgtcc gggaaatgcg ccgggcggtt
    28441 gcccgctctg tgcgcctgac agccaccgtg cggcactggt ttcgggagaa cggccagttg
    28501 tgggatatca acctgctgac ggctgtcacg gctccccgca ccggagtgga agagcgtgat
    28561 cttcttgtct gtcaggtgga gttttcgctg gatgcaaatc acggcgaaac cacccgtctg
    28621 attctggcac cccgtgacgg ctttattgtt ccggcagagc cgggaaacag cggaagcgga
    28681 aatgcgggtg acgtggacgc cttcgtgcgg gcacagatga aaaaacaggg gattaaattc
    28741 aatgatgaat gacgaagtca tcagccgcct tctggccccc gtgatgcggg gtgttcgtct
    28801 gctgttcggg cgtggtgtac tgaccggcac aacggacacg ctgaaaatcc agaatgtgca
    28861 aatcaccggt atggatggtg aaacctttga tgacgttgaa cgcccccagc agtacgggca
    28921 aatcagcgtc cccctgcctg gtgcggaagt ttttctggcc tgtgctggcg gacaacggga
    28981 tcaggccgtg gtgcttgtgg tggaagaccg ccgcagtcgc ccgaccggac ttaccgccgg
    29041 agataccggc gtgtatcacc atgaggggca ccgtatacgt ctgacaaaga acggccggat
    29101 cattgtgacc tgtaagacac tggagattta cgccgatgaa ggtgttcagg tggatacacc
    29161 ggaggctcac tttaccggta atatcacagt ggataagaac ctgcatgtca aaggcaatgt
    29221 gtccattgac ggcaccggaa gatcacaggg ggcgttcacg atgtccgaag cggttattgc
    29281 cgggatcacc tattcaggtc atgtgcatca cgataacggt gaaggcagca aaacgggagg
    29341 accagagaat ggctgatatt gctgtcgtct gggatcaggg ttgtggttcc ctgcaactga
    29401 acggcgcaga tcttctgacg gataacagcc tgctgactgc ggtcattatt tcactgttta
    29461 cggacaggcg ggcgctggat tccgatgaaa tccctgacgg cacccgtgac cgtcggggat
    29521 ggtggggaga cagtttccgg gagcgcccca ttggctcccg tctctggctg ttaagccgtg
    29581 aaaagacgct gtcctccgtg gtcagccgtg cacaggccta tgctgatgaa gcgctggcgt
    29641 ggctgcataa aagcggtgct gccacatccg tggtatgtca tgccatgcgt gtggggcatg
    29701 atcgcctttc gctttccgtg aaaatcaccc tgccggacgg aagcagacat ccgatgattt
    29761 tttatgctga tatgaagggg gaatgatgcc ttatcagccc ttaccactgg cgcaactgat
    29821 cacacagaca caacaggata tcagccagcg cctgcccggt tcgcagccgg gcgtgaatga
    29881 aaccaccctg aatgccattg cgtatgcact ggcgggtctg tcagcacagg agcatgaaca
    29941 tctggcctgg atctcccggc agataattcc gacagaagct gatgaagccg aactcctgaa
    30001 acactgcgca ttctggggtg tcatccgtaa accggcttcc cgcgctgacg gaccggtaca
    30061 actgatgctg accacggatg cagggatcac ggaaggcgta ctccttcagc gaagcgatgg
    30121 tgttgtgtac cgcatcaccg gctctgcgac cggaaaagcc ggaacactga atgttaatgt
    30181 ggaggcggaa agtgcggggc gcgccggaaa taccccgacc ggaacccgcc tgtcctttat
    30241 cacgccacag gcgggcatca accagacagc cacggttacc ggcacgggac tcaccggtgg
    30301 tgcggatgtg gaaacggtgc cggagttgct gtccaggctg gtattccggg tacagaaccc
    30361 gccatcaggg ggaacacagt atgattttga acgctgggca cgggaagtac cgggcgtgac
    30421 gcgggcatgg tgtaagcctg aatggcctga ggcgggtagt gttggtgtga cttttgttca
    30481 ggataataac cctgacattt tccccggaga aggtgatgtg aagcgggtgg cggattatat
    30541 ccgcagtcat gatgatccgg cgacgggcca gcccgttggt cagccacttg ggccgacaat
    30601 cagcgtgttt aagctgacca ataagccggt ggcgtttgag atcaggattg tacccaaaac
    30661 gccggaaaat caggctgccg taaaacaggc attaacggac ctgctttata acgaatcgcg
    30721 gccgggtgga cttgtattgc cttcatcatt ctggcgggct gttgcagggg tgaaaggact
    30781 ggaggatttt gaagttcgca gtccgctgaa gtccgtgatg gccggagata cagagttgct
    30841 gaccgtgggg gaaatcacat ggctgtaacc ctgaccccgc atcagcgcgc cctgttgcag
    30901 ttgctgcctg acgggctggc atgggataag cggccgtcat ccgttcttgc ggctttgtgc
    30961 ctgggcctca gtcattccac ggagcgtgtt tcctggaccg gtaaccagat gctggcagaa
    31021 cgttttcctg attcatcccg tctgctgctg gaagactggg agcgttatct ggggttaccg
    31081 gaatgtgata tgaccggcgc aaccattcag gaacgtcagc gttatgcagg gaataaatac
    31141 cggatgaaac cctctcttaa ccgtgaattt tatatccggt ttgcggcaga gtttggttat
    31201 gaaatagata ttcagccatc accggattca cagtgggtca gtattgtcac gataaacagt
    31261 gaaaccggct accggaatat gaatgtgctg gatgatattc tcaccccgct gcgtatttat
    31321 gagggtggtg cgctggaatg tattctgaac cgttataagc ctgcatggca gacgtttatt
    31381 tacgtgtatg caaacagtca caaagaggag aatatttaat gttccatgtt gataataatt
    31441 ccggcgtggc gaatatgcct gaactggctc cggcgcagag taataccacc acctggttta
    31501 ccgaaggtga cggacaaaaa ggtatcagct ggattggtca ggactggctg aatattctcc
    31561 aggccgaact gctgaatatt ctggctgaag ccagtattca gccggataag gcgcagttaa
    31621 accagcttac gctgtccatt aaagccatta tcgctgcgaa tgccttttcc cggaaaaaca
    31681 acctgaaaga aattgctgat gccggtgcgg aagcccagcg tctggcccgt ggttatcttg
    31741 gtctggggac gcttgccaca aaaaacagtc ttggtcccgc tgacgttaat gccctggcga
    31801 aggatcagaa ccttgccgac ctggagaatg cggaaaccgc ccgtaataat ctggatgttt
    31861 acagcaggag cgaaggtgat aaccgttacc tgcgcaggga ccagaatggc gcggatatcc
    31921 aggacaaagg aacctttatc gataacctcg gtttacggga aacggtaaat aaggctgctg
    31981 atgccctgcc atcggctgga accgccgtgg cagcaaacag actcgccacc ccaagaaata
    32041 ttaatggtgt tccttttgac ggaacgctgg atatcaacat cacgtcagga atgacgcagt
    32101 caaccgcaga tggccggtat gtacagaatg ttcagcttgg agcacagagc tatcattcac
    32161 ccggaggcaa tgaaatgtca tggaattaca gcgcaccttc cggttgtatg ctttccggta
    32221 ttaacgtgca ggaaaccggc tcccggtctg cggacaatat cggtggggtc tattatcgcc
    32281 cggttcagat ttatattaat aatacctgga gaacggtaag ttcagtgtaa accacagaaa
    32341 agggtgctga atgcaccctc tgaattattc cggtttttcc ggccagggaa tcgcctggta
    32401 atgcccctca ctggcaatat cattaaagtg catttcttta agctgtcgga tatacgccat
    32461 ccagcgcgtc agctgtaatt tattttcatc gctaatcatg tccagtttta attctgtttt
    32521 ccagtcatcc acggtttcgt tggcttcctg gagcagcgat gagcgacgct tttcagcaac
    32581 cgtcttccag tttaccggca cctttgatat caggccatca gaaaactgcc agttaccgtc
    32641 aatattcaca ccttccggca gttcgtccac ttcaacaaca gtaaaacctg ccggatataa
    32701 agcagagaca tcttcagcaa cagagcagat aatacccgtt acaggcgaaa tacagagttt
    32761 gtatttcttt gtaaaaagcg ggatagattc ataaaaatcc cttccgtcct cactctggaa
    32821 atactgaaca tcctcaccat agggtttgtc tttcgggtaa
//
LOCUS       DAZUCC010000032        32175 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZUCC010000032 DAZUCC010000000
VERSION     DAZUCC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.032"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..313)
                     /locus_tag="ACHY09_001332"
     CDS             complement(<1..313)
                     /locus_tag="ACHY09_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFS3884327.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KY"
     gene            complement(453..540)
                     /locus_tag="ACHY09_001333"
     tRNA            complement(453..540)
                     /locus_tag="ACHY09_001333"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(504..506),aa:Ser,seq:tga)
     gene            967..2085
                     /gene="hyaA"
                     /locus_tag="ACHY09_001334"
     CDS             967..2085
                     /gene="hyaA"
                     /locus_tag="ACHY09_001334"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFS3884328.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     KMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2082..3875
                     /gene="hyaB"
                     /locus_tag="ACHY09_001335"
     CDS             2082..3875
                     /gene="hyaB"
                     /locus_tag="ACHY09_001335"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFS3884329.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLSDDGSELISVQVR"
     gene            3894..4601
                     /gene="hyaC"
                     /locus_tag="ACHY09_001336"
     CDS             3894..4601
                     /gene="hyaC"
                     /locus_tag="ACHY09_001336"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFS3884330.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDVHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            4598..5185
                     /gene="hyaD"
                     /locus_tag="ACHY09_001337"
     CDS             4598..5185
                     /gene="hyaD"
                     /locus_tag="ACHY09_001337"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFS3884331.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPAKESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            5182..5580
                     /gene="hyaE"
                     /locus_tag="ACHY09_001338"
     CDS             5182..5580
                     /gene="hyaE"
                     /locus_tag="ACHY09_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFS3884332.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGS
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            5577..6434
                     /gene="hyaF"
                     /locus_tag="ACHY09_001339"
     CDS             5577..6434
                     /gene="hyaF"
                     /locus_tag="ACHY09_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFS3884333.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQQQISERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            6568..8112
                     /gene="appC"
                     /locus_tag="ACHY09_001340"
     CDS             6568..8112
                     /gene="appC"
                     /locus_tag="ACHY09_001340"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFS3884334.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8124..9260
                     /gene="appB"
                     /locus_tag="ACHY09_001341"
     CDS             8124..9260
                     /gene="appB"
                     /locus_tag="ACHY09_001341"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFS3884335.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9273..9365
                     /gene="cbdX"
                     /locus_tag="ACHY09_001342"
     CDS             9273..9365
                     /gene="cbdX"
                     /locus_tag="ACHY09_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFS3884336.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9445..10743
                     /gene="appA"
                     /locus_tag="ACHY09_001343"
     CDS             9445..10743
                     /gene="appA"
                     /locus_tag="ACHY09_001343"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFS3884337.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQCLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFSQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(10863..13043)
                     /gene="etk"
                     /locus_tag="ACHY09_001344"
     CDS             complement(10863..13043)
                     /gene="etk"
                     /locus_tag="ACHY09_001344"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFS3884338.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIVSVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEGFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13063..13509)
                     /gene="etp"
                     /locus_tag="ACHY09_001345"
                     /gene_synonym="yccY"
     CDS             complement(13063..13509)
                     /gene="etp"
                     /locus_tag="ACHY09_001345"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFS3884339.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(13497..14636)
                     /gene="gfcE"
                     /locus_tag="ACHY09_001346"
     CDS             complement(13497..14636)
                     /gene="gfcE"
                     /locus_tag="ACHY09_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFS3884340.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(14682..16778)
                     /gene="gfcD"
                     /locus_tag="ACHY09_001347"
     CDS             complement(14682..16778)
                     /gene="gfcD"
                     /locus_tag="ACHY09_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFS3884341.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(16778..17524)
                     /gene="gfcC"
                     /locus_tag="ACHY09_001348"
     CDS             complement(16778..17524)
                     /gene="gfcC"
                     /locus_tag="ACHY09_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFS3884342.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVVATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(17521..18165)
                     /gene="gfcB"
                     /locus_tag="ACHY09_001349"
     CDS             complement(17521..18165)
                     /gene="gfcB"
                     /locus_tag="ACHY09_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFS3884343.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18272..18577)
                     /locus_tag="ACHY09_001350"
     CDS             complement(18272..18577)
                     /locus_tag="ACHY09_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884344.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19018..19230)
                     /gene="cspH"
                     /locus_tag="ACHY09_001351"
     CDS             complement(19018..19230)
                     /gene="cspH"
                     /locus_tag="ACHY09_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFS3884345.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            19516..19728
                     /gene="cspG"
                     /locus_tag="ACHY09_001352"
     CDS             19516..19728
                     /gene="cspG"
                     /locus_tag="ACHY09_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFS3884346.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            19739..19927
                     /gene="ymcF"
                     /locus_tag="ACHY09_001353"
     CDS             19739..19927
                     /gene="ymcF"
                     /locus_tag="ACHY09_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS3884347.1"
                     /translation="MTPHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            19902..20132
                     /gene="ymcE"
                     /locus_tag="ACHY09_001354"
     CDS             19902..20132
                     /gene="ymcE"
                     /locus_tag="ACHY09_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFS3884348.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            20122..20295
                     /gene="gnsA"
                     /locus_tag="ACHY09_001355"
     CDS             20122..20295
                     /gene="gnsA"
                     /locus_tag="ACHY09_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFS3884349.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20343..21416)
                     /gene="yccM"
                     /locus_tag="ACHY09_001356"
     CDS             complement(20343..21416)
                     /gene="yccM"
                     /locus_tag="ACHY09_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS3884350.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDTPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLNFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLILGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(21488..24232)
                     /gene="torS"
                     /locus_tag="ACHY09_001357"
     CDS             complement(21488..24232)
                     /gene="torS"
                     /locus_tag="ACHY09_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFS3884351.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     DFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKANQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPQRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLLLRIATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAVALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDDIDIARANQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            24315..25343
                     /gene="torT"
                     /locus_tag="ACHY09_001358"
     CDS             24315..25343
                     /gene="torT"
                     /locus_tag="ACHY09_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFS3884352.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMAEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQEQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(25316..26008)
                     /gene="torR"
                     /locus_tag="ACHY09_001359"
     CDS             complement(25316..26008)
                     /gene="torR"
                     /locus_tag="ACHY09_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFS3884353.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26138..27310
                     /gene="torC"
                     /locus_tag="ACHY09_001360"
     CDS             26138..27310
                     /gene="torC"
                     /locus_tag="ACHY09_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFS3884354.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            27310..29856
                     /gene="torA"
                     /locus_tag="ACHY09_001361"
     CDS             27310..29856
                     /gene="torA"
                     /locus_tag="ACHY09_001361"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFS3884355.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGINAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            29853..30452
                     /gene="torD"
                     /locus_tag="ACHY09_001362"
     CDS             29853..30452
                     /gene="torD"
                     /locus_tag="ACHY09_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFS3884356.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATQTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDNLRQKTLTA
                     LRQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(30707..31012)
                     /gene="cbpM"
                     /locus_tag="ACHY09_001363"
     CDS             complement(30707..31012)
                     /gene="cbpM"
                     /locus_tag="ACHY09_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFS3884357.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(31012..31932)
                     /gene="cbpA"
                     /locus_tag="ACHY09_001364"
     CDS             complement(31012..31932)
                     /gene="cbpA"
                     /locus_tag="ACHY09_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFS3884358.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            32085..32175
                     /locus_tag="ACHY09_001365"
                     /pseudo
     CDS             32085..32175
                     /locus_tag="ACHY09_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7590 a         8247 c         8501 g         7837 t
ORIGIN      
        1 cgtattttgc ccgtgcgcgg ttacatgctt cattcgcgat gccattttcg ccggacattg
       61 cgaattcccg gcatgtggac ggacggttct tgtaaacaga gcaatatgcg tttttacccg
      121 gagttcccgc cagagcgaca catcggggat ttttctgact ggtgccgcgc atacagcgat
      181 gaaaggggga tatttgctca gtgagactga ccggaacctt tccgccagca tcgtcagctt
      241 ctgcccagta aaaagagacg cggaaaaatg cacaacaggc accacacgtc atgcatggat
      301 tggggttatt cataattcat cggtatagcg ataaatcgta aagagaacaa tgtacctagt
      361 gtgtattgag ctaaatatac caattagtat accaagtgta tactaagagc aaaaaaagaa
      421 aggggctacc atttggtaac cccttgattg tttggcggaa gcgcagagat tcgaactctg
      481 gaaccctttc gggtcgccgg ttttcaagac cggtgccttc aaccgctcgg ccacacttcc
      541 ggaatgacgc gcactataaa catcccgatg cggcgtgtaa acccctaatt tgtttgtttg
      601 cctgaaaaac agccaaaagt gcattgatag catgaaataa cagcagattg atcatttcat
      661 caccatgaat tccttctctt ttactcgttt agcaaccggc taaacatccc cactgcccgg
      721 caaaaagaaa aataggtcca tttttatcgc taaaagataa atccacacag tttgtattgt
      781 tttgtgcaaa agtttcacta cgctttatta acattacttt ctggcgacgt gcgccagtgc
      841 agaaggatga gctttcgttt tcagcatctc acgtgaagcg atggtttgcc ttgctacagg
      901 gacgtcgctt gccgaccata agcgcccggt gtcctgccgg tgtcgcaagg agaagagacg
      961 tgcgatatga ataacgagga aacattttac caggccatgc ggcgtcaggg cgttacccgg
     1021 cgcagctttc tcaaatattg tagtctggct gccacgtcgc tgggattagg cgcgggaatg
     1081 gcaccaaaga ttgcctgggc gctggagaac aaaccgcgca ttccggtggt atggatccac
     1141 ggtctggaat gcacctgctg taccgaatct tttatccgct ccgctcaccc actggcgaag
     1201 gacgtcatcc tttccctgat ttccctcgat tatgacgata ctttgatggc tgccgccgga
     1261 acccaggcgg aagaagtctt tgaagacatc atcacgcaat acaatggcaa atatatcctc
     1321 gcagtagaag gtaatccgcc gctgggcgag caggggatgt tctgtatcag cagcggacga
     1381 ccgtttattg agaaactcaa acgtgccgct gccggagcca gtgcgattat cgcctgggga
     1441 acctgcgcgt cctggggctg cgtgcaggcc gcgcgaccca atccgacgca ggcaacgcct
     1501 atcgacaaag tcatcaccga caaacccatt atcaaagtac ctggctgccc gccgatcccg
     1561 gatgtgatga gcgccatcat tacttacatg gtgacctttg atcgcttgcc agatgtcgac
     1621 aaaatgggcc gtccactgat gttttatggt cagcgtatcc acgataaatg ttatcgccgc
     1681 gctcacttcg acgccggaga gttcgtccag agttgggatg atgacgctgc ccgcaaaggt
     1741 tactgcctgt acaaaatggg ctgcaaaggg cctaccacct ataacgcctg ttcctccaca
     1801 cgctggaacg atggcgtttc cttcccgatc cagtcaggtc acggctgcct gggttgtgcg
     1861 gaaaatggtt tctgggatcg cggttcgttc tacagccgcg tggtcgatat tccgcaaatg
     1921 ggtacccatt ccaccgccga taccgtcggt ttaaccgcac ttggcgtggt ggcagcggct
     1981 gttggtgtgc acgcagtcgc cagcgccgtt gaccagcgca gacgccataa ccagcaacct
     2041 acagaaaccg aacatcagcc aggcaatgag gataaacagg catgagcact cagtacgaaa
     2101 ctcagggata caccatcaat aatgccggac gccgcctggt ggtcgacccg attacgcgca
     2161 ttgaaggcca catgcgctgc gaagtgaata ttaacgatca gaatgtgatc accaatgccg
     2221 tctcctgcgg caccatgttt cgtgggctgg agatcatcct gcaagggcgc gacccgcgcg
     2281 atgcgtgggc gttcgttgaa cgtatctgcg gcgtctgtac tggcgtacac gctctggctt
     2341 cggtttacgc catcgaagat gccatcggta ttaaagtgcc ggacaacgcc aatatcatcc
     2401 gcaacattat gctggcaacg ctctggtgcc acgatcatct ggtgcacttc tatcagcttg
     2461 ccgggatgga ctggatcgat gtgttagatg cgctgaaagc cgacccgcgg aaaacctccg
     2521 aactggcgca aagcctctcc tcatggccga aatcatcccc tggctatttc ttcgacgtac
     2581 aaaaccgcct gaagaaattc gttgaaggcg ggcagttggg gatcttccgc aatggctact
     2641 gggggcaccc gcagtacaaa ctgccgccag aagccaacct gatgggcttt gcccactatc
     2701 ttgaagctct cgatttccaa cgtgaaattg tcaaaatcca cgcggtcttt ggcggtaaaa
     2761 acccgcatcc aaactggatt gtcggcggga tgccttgtgc cattaacatt gacgaaagcg
     2821 gcgcggtcgg ggcggtcaat atggaacgcc tgaacctggt gcagtcaatc attacccgta
     2881 cggcggattt cattaacaac gtgatgatcc ccgacgcctt agccatcggt cagttcaaca
     2941 agccgtggag cgaaatcggc actggccttt cggataaatg cgttctcagt tacggcgcgt
     3001 tcccggacat cgccaacgac tttggcgaga aaagtctact gatgcctggc ggcgcggtga
     3061 ttaacggcga cttcaacaat gtgctgccag tggatttggt tgatccgcag caggtgcagg
     3121 agtttgtcga tcacgcctgg tatcgttatc ccaacgatca ggtcgggcgt catccgttcg
     3181 atggcattac cgacccgtgg tacaaccccg gcgatgtcaa aggcagcgat accaacattc
     3241 agcagctgaa tgaacaggaa cgctactcgt ggatcaaagc gccacgctgg cgcggtaatg
     3301 cgatggaagt ggggccgctg gcacgcacgt taatcgctta tcacaaaggc gatgcggcga
     3361 ccgttgagtc ggtcgatcgc atgatgtcgg cgttgaacct gccgctttcc ggcatccagt
     3421 caacattagg ccgcattttg tgtcgcgcgc acgaagcgca gtgggccgca ggcaagttgc
     3481 aatatttctt cgacaagctg atgaccaatc tcaaaaacgg caatctcgcc accgcctcca
     3541 ctgagaagtg ggaaccagca acctggccga cagagtgccg tggtgtcggc tttaccgaag
     3601 cgccgcgcgg agcgttaggc cactgggccg ccattcgcga tggcaagatt gatctctacc
     3661 agtgcgtggt gccgaccacc tggaacgcca gcccgcgcga tcctaaaggg caaattggcg
     3721 cttatgaagc ggcgctgatg aacaccaaaa tggcgatccc cgagcaaccg ctggagatcc
     3781 tgcgtactct gcacagcttt gatccgtgcc tcgcctgttc aacacacgtg ctgagcgacg
     3841 acggtagcga gctgatctcc gtgcaggtgc gttaacagcg aaggagaatc atcatgcaac
     3901 agaaaagcga caacgttgtc agccactatg tctttgaagc gccagtgcgt atctggcact
     3961 ggctgacggt gttatgcatg gcggtgttga tggtcaccgg ttactttatc ggcaagccgc
     4021 taccttccgt cagcggcgag gcgacgtatc tgttctatat gggctacatc aggttaattc
     4081 acttcagcgc cgggatggtt tttaccgtgg tcttgctgat gcggatctac tgggcttttg
     4141 tcggcaatcg ctactcacgc gagctgttta tcgtgccagt atggcgtaaa agctggtggc
     4201 agggcgtgtg gtatgaaatc cgctggtatc tgtttctggc aaaacgtccg agtgccgata
     4261 taggtcataa ccccatcgcc caggcggcga tgttcggcta tttcctgatg tcagttttta
     4321 tgatcatcac cggctttgcg ctgtacagcg aacacagcca gtacgctatt tttgcgccgt
     4381 tccgttatgt ggtggaattt ttctactgga cgggcggcaa ctcaatggac gttcacagct
     4441 ggcatcggct ggggatgtgg ctgattggcg cgtttgtgat cggtcatgtc tacatggcgc
     4501 tgcgtgaaga tatcatgtcc gacgacacgg tgatctccac catggtcaac ggctaccgta
     4561 gccacaaatt tggcaaaata agtaacaagg agcgttcatg agcgagcaac gcgtggtggt
     4621 aatggggctg ggcaacctgc tgtgggccga tgaaggcttc ggcgtgcggg tggcggaacg
     4681 gctgtatgcc cattaccact ggcccgagta tgtggagatt gtcgatggcg gtactcaggg
     4741 actgaacttg ctggggtatg tcgaaagcgc cagtcacttg ttgattctcg atgccattga
     4801 ctacgggctg gaaccaggaa cgctgcgaac ctatgccgga gaacgcattc cggcttatct
     4861 cagcgcgaag aaaatgagcc tgcaccagaa cagcttctcc gaagtgctgg cgctggcgga
     4921 tatacgtggt catttgccag cacatattgc cctcgtcggt ctgcaacccg caatgctcga
     4981 cgactacggc ggtagcctga gcgaactggc gcgggagcaa ctgcccgctg cggaacaatc
     5041 ggcgctggcg cagctggctg cgtggggaat tgtgccgcaa ccggctaagg aatcgcgctg
     5101 tctcaattat gactgtctgt cgatggaaaa ttacgaaggc gttcgcttgc gtcagtacag
     5161 gatgaggctg gaggaacagg gatgagcaac gacacaccat ttaatgcgtt gtggcaacga
     5221 atgctggcgc gcggctggac gccggtcagt gaatgccgtc ttgacgactg gcttacgcaa
     5281 gcgccagacg gcgtggtgtt attaagtagt gacccgaaac gcacgccaga agtcagcgat
     5341 aacccggtaa tgattggcga attactgcgc gagtttcccg actatacatg gcaggtagcg
     5401 attgctgatc ttgagcagag cgaagccatc ggtgatcgtt ttggcgtatt tcgttttccc
     5461 gccacactgg tgtttaccgg tggtagctat cgcggcgtgc tgaatggtat tcacccgtgg
     5521 gcggaactga taaacctgat gcgtgggctt gtcgaaccac aacaggagcg tgcctcatga
     5581 gcgaaacttt tttccatctg ttggggccag gaacgcaacc gaacgatgac agtttcagca
     5641 tgaatccact gccgatcacc tgtcaggtga atgatgaacc gagtatggcg gccctggagc
     5701 aatgtgctca cagcccgcag gtgattgcgc tgttaaacga gttacaacaa caaataagcg
     5761 aacgccagcc accgttgggc gaggtgctgg cagtcgatct gttaaatctc aacgccgacg
     5821 atcgtcactt tatcaatacg cttctcgggg aaggggaagt gtcagtgcgc atacagcagg
     5881 ctgacgacag tgaaagtgaa atacaggagg cgatcttctg cggattatgg cgggtgcgca
     5941 gacgtcgcgg cgacaagttg ctggaggaca aactggaggc tggctgcgcg ccgctggcat
     6001 tgtggcaggc ggcaacgcaa aacgtcttgc cgacagattc gctgttaccg ccgcccattg
     6061 atggcctgat gaatggccta ccgttggcgc atgagctact ggcgcatgta cgtaaccccg
     6121 acgcgcagcc gcacagcatt aatctgacgc aattacccat cagcgaggct gatcggcttt
     6181 ttctctcacg tctctgtggg ccgggaaata ttcagattcg caccattggc tatggcgaga
     6241 gctatatcaa cgccacgggg ttacgccatg tctggcattt acgctgcacg gacaccttaa
     6301 aaggcccgtt actggaaagt tatgaaatct gcccaatacc ggaagtggtg ctggcagcgc
     6361 cagaagattt ggtcgactct gcgcagcggc ttagcgaggt atgccagtgg ctggcggaag
     6421 gtgcaccgac gtaaaaagac ggtaagtatc gctttcaggc ttatgaatat cgcaatcggc
     6481 gaatacctct ggtcgtagag tttcaggata aagagggaga tctaccatta acgggttatt
     6541 tttctctctt cgcctacagg agtgcgcatg tgggatgtca ttgatttatc gcgctggcag
     6601 tttgctctga ccgcgctgta tcacttttta tttgtacccc ttaccctggg gctgattttt
     6661 ttgctggcta tcatggaaac catttacgtg gtcaccggca aaacaatcta ccgcgatatg
     6721 acgcgcttct ggggtaagct cttcggtatc aactttgctc ttggcgtggc taccggcctg
     6781 acgatggagt ttcagtttgg tactaactgg tcattctatt ccaactatgt gggcgatatt
     6841 tttggcgcac cgctggcgat ggaagcatta atggccttct tcctcgaatc cacctttgtc
     6901 gggctgttct tcttcggctg gcagcggctg aataaatacc agcacctgct ggtgacgtgg
     6961 ctggtggcgt tcggttcaaa tctctctgca ttgtggatat tgaatgccaa cggttggatg
     7021 caatacccga ccggtgcgca ttttgatatc gacaccctgc gtatggagat gaccagtttc
     7081 agcgagttgg tctttaaccc ggtcagccag gtgaaatttg tgcacactgt aatggcgggc
     7141 tacgtgaccg gggccatgtt tattatggcg atcagcgcct ggtatttact gcgcggacgg
     7201 gagcgcgatg ttgcattacg ctcgtttgcc atcggttccg tcttcggtac tctggcgatt
     7261 atcggcacgc tgcaactcgg agacagttct gcgtatgaag tcgcgcaagt acaaccggta
     7321 aaactggcgg cgatggaagg ggagtggcaa acggaacctg cacctgcacc gttccatgtg
     7381 gttgcctggc cggaacagga tcaagagcgt aacgcctttg ctatcaaaat tcccgcgctg
     7441 ctagggatcc tcgccactca ctcattagat aaacccgtgc cgggtctgaa gaatttgatg
     7501 gctgaaacct acccacgctt gcaacgcgga cgtatggcct ggctgttaat gcaggaaata
     7561 tcgcaaggca atcgtgagcc gcatgtgttg caggcatttc gggagctgga aggcgacctt
     7621 ggttacggca tgttgctctc ccgctatgcg ccggatatga accatgtcac agctgcacag
     7681 taccaggcgg cgatgcgtgg cgcgatacct caggttgcgc cggtattctg gagtttccgc
     7741 atcatggtgg gctgtggttc tctgctgcta ctggtgatgc tgattgcgct tgtccagacg
     7801 ctgcgtggca aaattgacca gcatcgctgg gtgctgaaaa tggcgctctg gagtttgccg
     7861 ctgccgtgga ttgcgattga agccgggtgg tttatgaccg agtttggtcg tcagccgtgg
     7921 gcgatacagg acatcttacc gacatactcc gcgcactccg ccttaaccac aggacaactg
     7981 gctttctcac tgatcatgat cgtagggctt tacaccctgt tcttaatcgc cgaagtctac
     8041 ctgatgcaga aatatgcccg tctggggccg agcgcgatgc agagtgaaca accgacgcag
     8101 caacaggggt aaaggagaaa atcatgtttg attatgaaac attgcgcttc atctggtggc
     8161 tgctgattgg cgtgatcctg gtggtcttta tgatctccga cggatttgac atggggatcg
     8221 gctgtctgct gccgctggtg gcgcgtaatg atgatgaacg tcggatagtg ataaacagcg
     8281 ttggcgcgca ctgggaaggc aatcaggtct ggttgatcct cgcaggtggg gcattatttg
     8341 ccgcctggcc cagagtgtat gcagcggcgt tttccggctt ttatgtggcg atgattctgg
     8401 tgctgtgctc gctgttcttc cgcccgctgg cctttgatta tcgcggaaaa atcgccgatg
     8461 cacgctggcg taaaatgtgg gatgccggac tggtcatcgg cagtctggtg ccgccggtag
     8521 tcttcggtat tgcattcggc aacttgctgc tcggcgtgcc gtttgctttc acaccgcaat
     8581 tacgcgtgga atatctcggc agcttctggc aactgctgac gccattccct ttattgtgtg
     8641 gattgctcag tcttgggatg gtgattttgc aaggtggcgt ctggttacaa ctgaaaactg
     8701 ttggtgtgat tcatctgcgt tcacagctgg cgaccaaacg cgccgcacta ctggtgatgc
     8761 tgtgcttttt gctggcgggt tactggctgt gggttggtat tgatggcttt gtactgctcg
     8821 cccaggatgc taacggtcct tccaatccgt taatgaaact ggtggcagtg ctacctggtg
     8881 cctggatgaa taatttcgtc gaatcgcccg ttttgtggat cttcccgctg ctggggttct
     8941 tctgcccatt gctgacggtg atggcgattt atcgtggtcg cccgggttgg ggatttttga
     9001 tggcgtcatt gatgcaattt ggcgtgattt tcacggcagg catcacgctg ttcccctttg
     9061 tcatgccgtc aagcgtgagt ccgatctcca gcctgacgtt gtgggacagc acttccagtc
     9121 agctgacgct gagcattatg ttggtaatcg tgctgatatt tttgcctatt gtgttgctct
     9181 acactctctg gagctactac aaaatgtggg gacgcatgac gacagaaact ctccgccgta
     9241 acgaaaacga gctgtactaa ggagcaggaa caatgtggta tttactttgg ttcgtcggca
     9301 ttttgttgat gtgttcgctc tccacccttg tgttggtatg gctggacccg cgtctgaaaa
     9361 gttaacgaac gtaagcctga tgtggtgcat tagcatcgca tcaggcaatc aataatgtca
     9421 gatatgaaaa gcggaaacat atcgatgaaa gcgatcttaa tcccattttt atctcttctg
     9481 attccgttaa ccccgcaatc tgcattcgct cagagtgagc cggagctgaa gctggaaagt
     9541 gtggtgattg tcagtcgtca tggtgtgcgt gctccaacca aggccacgca actgatgcag
     9601 gatgtcaccc cagacgcatg gccaacctgg ccggtaaaac tgggttggct gacaccgcgc
     9661 ggtggtgagc taatcgctta tcttggacat taccaacgcc agtgtctggt ggctgacgga
     9721 ttgctggcga aaaagggctg cccgcagcct ggtcaggtcg cgattattgc tgatgtcgac
     9781 gagcgtaccc gtaaaacagg cgaagccttc gccgccgggc tggcacctga ctgtgcaata
     9841 accgtacata cccaggcaga tacgtccagt cccgatccgt tatttaatcc cctaaaaact
     9901 ggcgtttgcc aactggataa tgcgaacgtg actgacgcga tcctcagcag ggcaggaggg
     9961 tcaattgctg actttaccgg gcatcggcaa acggcgtttc gcgaactgga acgggtgctt
    10021 aatttttcgc aatcaaactt gtgccttaac cgtgagaaac aggacgaaag ctgttcatta
    10081 acgcaggcat taccatcgga actcaaggtg agcgccgaca atgtctcatt aaccggtgcg
    10141 gtaagcctcg catcaatgct gacggagata tttctcctgc aacaggcaca aggaatgccg
    10201 gagccggggt ggggaaggat cactgattca caccagtgga acaccttgct aagtttgcat
    10261 aacgcgcaat tttatttact acaacgcacg ccagaggttg cccgcagtcg cgccaccccg
    10321 ttattggatt tgatcatggc agcgttgacg ccccatccac cgcaaaaaca ggcgtatggt
    10381 gtgacattac ccacttcagt gctgtttatt gccggacacg atactaatct ggcaaatctc
    10441 ggcggcgcac tggagctcaa ctggacgctt ccaggtcagc cggataacac accgccaggt
    10501 ggtgaactgg tgtttgaacg ctggcgtcgg ctaagcgata acagccagtg gattcaggtt
    10561 tcgctggtct tccagacttt acagcagatg cgtgataaaa cgccgctgtc attaaatacg
    10621 ccgcccggag aggtgaaact gaccctggca ggatgtgaag agcgaaatgc gcagggcatg
    10681 tgttcgttgg caggttttac gcaaatcgtg aatgaagcac gcataccggc gtgcagtttg
    10741 taatgcataa aaaagagcat tcagttatct gaatgctctg aggctgatga caaacgaaga
    10801 actgtctaat gcgtagaccg gaaaaggcgt tcacgcccca tccggccacc ttaccttcag
    10861 ttttactctt tctcggagta actataaccg taatagttat agccgtaact gtaagcggtg
    10921 ctggcgcgtt taatcacacc attgaggatt gcgcctttaa tattaacgcc agcctgttcc
    10981 agacgctgca ttgacaagct cacctctttg gcggtgttca agccaaaacg cgcaaccagc
    11041 aggctggtgc cgacagagcg tcccacgacc gcagcatcgc tcaccgccag catcggcggc
    11101 gtatcgacaa tcaccagatc gtaatggtcg ttcgcccatt ccagtaattg acgcatccga
    11161 tcgcgcatca gcagttcaga cgggttaggt ggcacctgac cgcgagtaat cacatcaaag
    11221 cctcctttgc caaaatgctg gatcactttg ttgagctcat ctttacctgc cagatattcc
    11281 gacaagccat gttcattact cacggtaaac aggttatgcg aataaccacg gcgtaagtcg
    11341 gcatcaataa ataacacttt ttgatcggac tgggcgatca ccgctgccag agttgaactg
    11401 acgaacgttt taccactgtc tggcgtcgca ccggtgatca tcagaatgtt attctctgtc
    11461 tccatcatag cgaagtgcag actggttcgt agcgcacgta cggcctccac agcagaatcc
    11521 gccgggttat ccaccgccag gaaggggata tttttagtac gatggcgctg ctgattagaa
    11581 aataaatttt tcttacgcag acgggtacgt ttatccagcc actcggacat tgggatagtg
    11641 gcataaacgc tgatgccgtg ctcttccagt tgttccgggg cttctacacc acgacgcaac
    11701 atcgcacgcg ccagcacggc acccaccgaa ataaacaggc caagaataaa accaagcacc
    11761 acattcaacg cttttttcgg tttcactggc tgcggctgag tgactgccgg gtcgataatc
    11821 cgcacgttac caatcgcact ggatttcgaa atactcaact cctgctggcg gttaagtaat
    11881 tgcagatata ccgcacggcc cgcttctacg tcacgactta aacgcaacac ttcctgttgg
    11941 gtggaaggca ttgccgatac ccgcttattc aggcgtttgc gttcttgctc cagtgtctgg
    12001 cgtttttcca gcagcgcacg ataagttggg tgatctttct tatacagctg ggagatctct
    12061 gcctcgcgga aagtcagctc attgagttga ttatcaacgt tcacaatctg ctcaagaacg
    12121 gctttggctt ccaggttaag gtcaaccgaa tcgcgctgtt ggcgataaac gttgagtttt
    12181 tcttccgcct ggtccagctc gctgcgcact tctggcaact ggcgctgtaa gaattcaagg
    12241 ctttgtgaat cctgcgccgc ctggcgagcg atattctgtt gcaaatagtt gttagcgatg
    12301 ctgttcagaa tacgagtaat caactgggga tcatcaccgg tcatggtaag ttccagcatc
    12361 ccgctttctt tactgcgttc gctaacggta aaggtttcct gcaacgcgtt aatcgcttcc
    12421 agttcggtac gctggctcag gacaaactgc gttcctggtt tggccttaat gtccgcgata
    12481 gtcagcgcaa cgccgtcttt ttccagacgc tggccgacca taccattgac ggtgaaccct
    12541 tcaccttcca gcgtatagtg gccgttttcc ccaactgtga gtgtcagttg ctgatcctga
    12601 ccattcagtt gtgggatgtg catccagctg atcgccagct cacctggttt ctctttggtt
    12661 aatctcgccc agccgcgacc cacaatcgga aaatacttct gctcaaccat gtcgcgcaga
    12721 ttcagttcag caatggtttt accgagaatc atgcgcgatt gcagcagttg gatctccggt
    12781 gctgactcgg gcgatgagtt agggatcata tcgctcaggc cgctgagaat ggcgttgccc
    12841 tgtttctgct caacctggac cagagtatct gcctgataaa ttggcgtgct taacagcgag
    12901 taagcgacag cgatcagcgt gaacaacgcg gtcacgctga caataaactt acggtgatcc
    12961 cataactcgc cgaccagacg aagcagatcg atctcatttt cctgagtgct gcctggtggc
    13021 gtattcatat ttttagttgt catcgtattt tataaactca aattaccggc tgaggcgctt
    13081 cgcccattcc tgactggcgc gctccaacat accgtagaca tgctcaaatg cgtcctgact
    13141 tttacgatag ggatccggga tctctttctg ttccagccat tgcccaaaca gcattgtctt
    13201 cccgcggacc tcgggagcga ttgccgtaac ctgagcaata tgttccgact ccattgccag
    13261 aatcagatcg taatttcgtg ccatctcagc agtgagctta cgtccggcat gtccttcaag
    13321 agatacgcca tgattagcgg caacatctgc cgctgtcgca tctgcagggt gctttaccag
    13381 accatgaaca cctgccgatt tgacttttac gcctggcaga cgtttacgca gcaagcgctc
    13441 gccaatgggc gaacggcaaa tgttgccggt acaaaccacc aggattgagt taaattttag
    13501 ttgggccatg tttgaatcca ccgtgaagtc tctgtcaggt catgcacccc ggaaatcgtc
    13561 ggtaccagta gagaaatcac acggttccaa cgagccagcg gcgcagtagt gacataaaca
    13621 atgtcgtaag gttcaagctg gaattccgtt ccgagtatca ttgccgatgc gtccttcgca
    13681 ttcagctgat aaatattggc gattttgcca ttctgcttgt tttgcgttgc acgaataaca
    13741 aaaatccccg tcgcatcagc gacatcctga ttcataccct cagcgttacc cagcgcttcc
    13801 gccagggtca taccgctgcg gtccattttc agcgtgctct gtttacccac ttcacccatc
    13861 acgaagactt tcagatcatc gttgcgcgga ataaataaaa tatcgcccgg gtgcagcagt
    13921 ttgttctggc gcaagtcgcc acgctgcatc agcgcataga ggttcacttt ggtctttacg
    13981 ccattctggg tgagcaccac gttgcgccag tctgcatctg cggtcagccc ccccgcggcg
    14041 ttaatcgcat ccatgatggt cagaggaata ttggtaatcg gctgctgacc cgatttgctg
    14101 acttcaccgg tgacgtacgc tttttgcgag cggaacgccg cgacgctgac atcgacctgc
    14161 gggctttcaa tgactgaatc cagacgcgct gtaatctcgt tgcgtacctg ggtaagcgtt
    14221 ttcccggcca ctttcaggcg accaatatag gggtagaaaa ttgcaccatc agcgttaacc
    14281 cagttgccgg tatcactggc gctacggtat tgccctgcgg gtgtggttag ttccgggtga
    14341 tcccagaccg taaccatcaa cacatcacca atgccgatac gatattcata gttcgcgatt
    14401 tgctgctcca gctccgggtt agcctgcgac ataatcggtt tggcgcgcag ctgatcaatc
    14461 aatcctggcg tgacgggata gatattgacc attttgtcca ggtcatactg attgtcaggt
    14521 agttcaatga catccttgtt actggtcgat aaattctgac caggaacaag ggtacatgct
    14581 gtcaaagaca gaacagccaa tgtcagtacc gaaaacttaa aaatgttctt tttcattggt
    14641 ttatatttat cagtcattgt aatgttatga attacgtcag gttattgtcc aaccggtact
    14701 tctcgctctg ccgtcattgg atagagctgg tatttgcgtg acagcatttg tccaccatca
    14761 cgcgtcaatg gtgtccacga aaccaccgcg cggttgcggt taggtccaat ggtcatcaag
    14821 tcgaacggaa tcgagatata aaaaccttta ctaaagccgc cttcgccgta gtcatctttt
    14881 gatacgttac tgattgccgc ccatacccct actgccacgc cgctgtcaaa gcgtttggcg
    14941 acgtcgatcg ttgcaccttt atcttttgcc agatattgcc caacgctaag tttcatcagt
    15001 acgccattga gcgtcggcgg gttccagtaa gccgtcacga aacctgttgg cgtagaataa
    15061 tcggtgaagc gcatcatgtt gtcccagtca cgctgcttca cgtagttaac gtccacaccc
    15121 agcgcccagc tggcatctag cgggcgataa agcagctcgg aaccgacacc ggcatacatc
    15181 gtttccagat aaccgccata cacctggcca tagaaaccgt tgcctaagtc ggcaaagtag
    15241 ttcgcttgca agttgttgag ataaacgtca ttgcgaacgt aatcacggat atgcgtgcgc
    15301 acgcggggca gggtagagtc cgcgggtaat agcgaagact taaacttgtc gtagttattg
    15361 taaatattgg tgaaaatacc gccatcaagc agcaggtggt cggtaaacca gtagcgggcg
    15421 ctggacatca gccccagctg gaacatatag aaatcttccg ggccgcccag cgactgtgaa
    15481 agtgttgggt tgaaagagta gctaaagcga tcttcacgaa tacggtagcc ccgaccaaag
    15541 gcagaaagat cttcagcttc aacacgttgt tgttgcagtg gctctgattg accaggcgct
    15601 gtaccggcca gttgtttgcg caggctggct acgtcggttt cggtagtcac catcgccata
    15661 tgctcgcgct tttgcgtcac gctaatcttc tcaacgcctt gcggcaggtt attcaccaga
    15721 atccggttgg cacgatcgac cgcttcgcga gagtcacggt atttgtattg ctgaccagac
    15781 atatacagcg tcttatcgcg tagctgaatt tctggcgcgt caaagcccgc gttatacttc
    15841 agggcggtaa gttggtttgc taccgtggtg tactgcaatc cttcagattc cggcgcaggt
    15901 tgatatgccg gttttggcgt atcgcgcagg gcagggcgca gatcgttgaa attggtccgt
    15961 aacgtgaagc caaacatcaa cgtgttaccg cgttcataac tcaggttgag atctgcccag
    16021 ctggcagcgc gatagactgc gccgacgttg aaatggcttg cctgaggcag tttgcccgca
    16081 aaatcattct ggtaattgtt gccatcgtat tcgagtttca gacgcagagg attccacggc
    16141 gtttgatact caataccgcc aaaaatagag gctggaccac gaaagatatc gctaaagctg
    16201 atatctcccg catcgtgaga ctctgcgcga tggcaatatt tatcgcttac ccgacaaaac
    16261 gggttggtaa tattgcccgc attaccggcg tagccccagg ccatcccgag ggtgaaatcg
    16321 aacgggcccg cttgcttgct ggccaccaaa tattcaccgt caaacagacc cgtaccagca
    16381 atatcacgtt taccaaacgc cacttgcggt agccagtaac cttcttccca caggcgaagt
    16441 ttaaaatcga aagatttgtc tttgtatgac tgatcgccgc tgaaatcttc ccactggcta
    16501 tatttgcgtg tgcgcacatc cgtataacga atggtgcctt ccagccatgg gaacagcgcc
    16561 acggaggtgg agtagaaacg gtattgatcg ttatcccggt agtttacgct gaattcacct
    16621 tccggtgcga tgcgcgcatt tggcatctgc aacaatcctg tgccgccaaa gtctgattgc
    16681 gacgaaccca gcggatccgg ataggttaat acttcagcat gacaggcact ggagacggca
    16741 atggccaggc agcttaaaag ataagagttc ttcttcatca gtcaggaaca cgttgcgtta
    16801 aaacagacac aatctggtca tttaaatcag catatttttc gggcagtaca tgcgcagaaa
    16861 agcctaacca aagctggctg ccaggcggtg gttccacatg tcttttattc cacagggcaa
    16921 caggggcgac gacagcctca ccctcaggcg taatcaccat aacgttattc ttgtccgcgc
    16981 ctgcaagacg ggtgtgatct tgcagataat cggtaacgct acggcctgcc agccagggca
    17041 attgcccagc cccagaaacc gcacctagca gagtaatagt caccggacgc tgtacggtat
    17101 agagcgtata gtccccgacc agcgggggat tgctgttctc atccactcgt acaaaatcag
    17161 ggtccagttt gaccggtaag cgaccagtga tattgagatt gagtaactgc tggcgcacgg
    17221 atttaatcgt tgccaccaca tcgtcgtcag cttctgcttc ccaggaagca agctgtgcca
    17281 tgacatgttg gtagtcttta agcgctttag ctttcgccgc agagtcggtc agcaaggcac
    17341 caggccacca gaggcgatcg cgcagttgcg gttgtgtcac caactggaca acattttcca
    17401 cggggccaac ggaaagtgtc tgttgttcgc caggcagata aatagtcacc gttccttgcg
    17461 caaaggcatg gggtgtcatt acgtaaagta cgctggcaat gaaatacgac tgtaatttat
    17521 tcatggtgcg ggtttaagaa acgtcatttc tacgggaata acccctgcgc ctaacatttg
    17581 ccgactttga cgaacctggc cagtagccga atcgacccag aatgtgtttt gccactgttt
    17641 ttctggcagc gtggaagtca cctcttcctg ccagacatta cacagcacat cgcgacccgc
    17701 aatattcagc gtctctaatc cagcaaagga aaaagtggaa ctcagggtcg cagagcgaaa
    17761 atggttgtct tcactccaga gaatatcgcg cgtccagcgg ctgccgtctt ttattgccag
    17821 cgcgttacgc agagggtcct gcccggaatt tgtcacttcc agcagattat tattaagttt
    17881 gacggttttt aaaagacgtc cattgtgggt aaccagcatg gcgttatcct gggataacca
    17941 tttgctttgt tcttgttcta tatatcccag taccacaaag attcgttgcc cttcattaag
    18001 gcgtaaatac atcgtggaat aaggcaacgc ctggatctgc tgatccgcta cggtgatatc
    18061 ctgattatcg aaaaggctgg cgcgaaatgt atcgaccata ctttgctggc tgtgcgtaca
    18121 tcccgcaaga aatagtgcga aaatcgataa aataagaggg cgcaccttta atccttgaaa
    18181 ggttaattaa aaaaatgcaa atctctaaag cccggtacga atatcgcaac cgggtatcat
    18241 ttaactttaa ttttgacgcg ttaaagcagt attagcgcgt agtggacgtg gtggttgagg
    18301 tgttatgaga accgccatcg ccactgtcat ttgctgcaac tactgccaac gtcgccacac
    18361 caacagccgt tgccacaccg acaaccgtcg ccgtgccgac agcgccagca gccacgccac
    18421 cagtggtagc agcagtcgtg gttgcacctg tagtggtcgt tgtggtgcca gtagtacccg
    18481 tcgttgcctc agtcccgttt gctgcttcag gggcagcaaa tgcagctggc gtcgtcaaca
    18541 tgaaggccat aaggattgca gaaagcttgt gtttcataac ttttccttta ttcatcgcat
    18601 ggacaatacg tacgcttgag atcaggatta tctactttga gtataacatc cttgtgaaat
    18661 ctccctttcc tcataccgcc agcactatat actgcgggtg ggaaaggcgc gcagagggaa
    18721 atataagatt gttaactatt cccctctgga aatacgactt gtgagcataa gataaatctt
    18781 agcaaaatac aaattaaaga taagaaaatt gttaagaaag ctattaacta ttgttggtaa
    18841 tatagtttca agtggtacta taactggctg ttaagtggca tttataacta ttaggtgcag
    18901 agatattcgc ttaaatggat ttgttgctcg gtgaaaaagt aacttgcgtt ataatttatg
    18961 gtgttgacta ttgagctatt ccaataaaac cagccaactt taaacaagca actcatatta
    19021 agagagataa acatttgccg ctgttggtcc tcgcaggcca tttacgcggc aaaattccac
    19081 gcgtaatcct ggtataagca cttctgcgtc gcggggagtg aatgcggaaa tatggacctg
    19141 gacttcttta cgaccgtcgg aggggataat gaatcctttg ccgcttttgc gatcaaaggt
    19201 tttgacaatt cctgtcattt tacgggacaa acaaattcct tactgaaaat actgcgctgc
    19261 actatacggg gttaataaaa taaagccagc gatatttaag accgccggac ggctaaaata
    19321 aaatttgctt aatctcaatt atcatgcgtt aatagctgcg tcggtttgaa agacagacag
    19381 catacaaagt agtttactaa agcagttctc attatcaggc attatcccct tcttttgagt
    19441 ctctctcctg aacactaagt agtttctgta ctaaagccct gtttgccgaa aggcccaaaa
    19501 tgaaggaagt aaaatatgtc taataaaatg actggtttag taaaatggtt taacgcagat
    19561 aaaggttttg gctttatcac tcctgatgat ggcagcaaag acgttttcgt ccatttcacc
    19621 gccatccaga gcaatgaatt ccgcacgctg aacgaaaatc agaaagttga attttctatt
    19681 gagcaggggc aacgtggtcc cgcggcagcg aacgttgtta cgctctaagg ttgccgttat
    19741 tactccacat ctccatttcc gctgtccatg ttgtcatggt tcacagtacc gcacatcggc
    19801 attcgatgtg acggagcgaa atcctttagg ggcaaagtgt attttttgta aatcgacgat
    19861 gatcaccttt gataacgtcg cgctgcaaat acgcactgac catgcgccgc tggatttcac
    19921 aaaataatat caggctccct cgtggagcct tttttatatc tgccttattt ttcttcaacg
    19981 ctgtatgtat agtaagcgat aacctgttga ttattgaatc tttcggggag atggcttata
    20041 acatttctta cctgaccagg gtaccgggaa ccaacacctt actggcgtgt tgctgtcttt
    20101 caagaccaga agaggttaac agtgaatatt gaagagttaa aaaaacaagc cgaaacggaa
    20161 atcgctgact ttatcgcgca aaaaatcgcc gagctgaaca agaatacagg gaaagaagtc
    20221 tctgaaattc gcttcaccgc acgagaaaaa atgaccgggc ttgaaagtta tgatgtcaaa
    20281 atcaaaataa tgtgattttg tgaacatcac ccgtgcgagg tgatgttccg cttgttgcta
    20341 atttagtggc caatcattgg cgcttgtgga attaagcgtc ggtacaattc ctccggcacc
    20401 gggctttgcc atactcccgc atacattgcg taaccaatca ccgcaaacat aatccccaga
    20461 atcagtagcg tcattaacca gccagacaac gcaaaggctt ttttatttgc cgcaggtttt
    20521 tgcagtgaaa aggtcaatgt tgaggctacc ggacatgact ctacgcaagt catacagccg
    20581 gtacattcca ctgttcgtac ctgaattaat ttatcgaccg ggatccgtga tgggcaattt
    20641 tttgcgcatt tgccacagtc gatacaactt tcggcattgc gacgaatctt aaacggcgat
    20701 aatagcgaaa ccacgcccat cagcgcgcca tatgggcaaa gataacgaca ccaggcatgg
    20761 cgaataaaca ggctggcaat cagcaaaacg atcatgctga ttaatgtcgc ggtccccata
    20821 tgacgaaaga aattgagcat tttaacgtcc atcaccacgc tgtagggcga caacataaaa
    20881 tagtgaatcg cctgagcggg catcaataac gcaatataga gaaaaaaact caacagcaaa
    20941 tacttcacgc cgcgcagagg agtatccagc cagcggggaa ggacaaattg ccgaccaaac
    21001 agtttgttac cgagatcgcc gattaattca gaaagcgtac caaccgggca taaccatgaa
    21061 caaaaggcct tttttaataa tagactgatg acgataaaag cgaccaataa cagcatcgcg
    21121 gcggcgtgga cggacggtaa ctggcctgtt gcaaggctat atttcagatt catcagaccg
    21181 gcaatcggta gccagccttc gatacctccc ggtctggtga caaatgtcgt actacctgcc
    21241 gtttcgtaat agcgcaccca ataccagaac gtgatggcaa tataaatatt cattgccaac
    21301 agtaataatt gcgtcgcttt acgccaggtc gtggcattgc gccagtcatt ccacggtaat
    21361 ttgccgcccg tcgtgcctgg ccgccgctgc cagcgggttc ttcttttctc tgtcatgatt
    21421 ttgccagtcc gttaagttgc ataccaaatg ccactattct agttgttctt aactggctga
    21481 tattgattca aatcgcgttc aggtctttct tatgcaacca tgcttccaga gcggcaacac
    21541 tgcgtgtaat ttcttcgtgt ggaagggggg cagataatgg ctgctgctcc agttgtgcgc
    21601 atagctggct ggcgatacgc attcccaggc tcgagcaact gctttttagc tgatgtgcgg
    21661 cacgctttat tttctcgcta tcctgattgg cgcgggcaat gtcgatgtca tcgagaagcg
    21721 gcagggcatg ttgtgtaaat aataccagcc attcgtggat cttctccgtc cccattaact
    21781 gagcatcttc attgagttgc gatacatcca gcggttgatc gttattgact tgcagttgga
    21841 gatagtgcgc cagtaactga ccgagcactt cacgcggcac cggtttaggg ataatcccgc
    21901 gaaatagcga actggtacgc tggcgcaacg tttcgtcgat gacatgggcg ctaaagccaa
    21961 tcaaaaccag cgacggatat tgctgtgcca gttgccgggc aagcgtaatg ccgtcgatat
    22021 ccggcagatc aaaatccacc agtgcgacag caaatggttc actattttgc agtgtctcta
    22081 aagcctgcgc ggcatttcct acggcaacga cctgcgcccc actgctgttc aacatctcaa
    22141 cggtaatccg ctgggttagc gggttatctt caattaacag caaacgtaaa ccgtccagac
    22201 gcaccgcctg attgactgtt tttggcacgg gtgcagtggc aatacgtaac agcaagcgta
    22261 aacaaaaaca gcttccaacc tccggcgtgc tggtggcgct cagttcgcca cccatcgcct
    22321 gggccagacg gctactgata gtcagcccca gcccggtgcc gccgcgtttg ccgctaacct
    22381 gaacaaatgg ctggaagatt tcagccagtt tcgcgggatc aataccgcag ccgctgtctt
    22441 ccacttcgac cagccattgt tcgccatcag tgcgactacg caggataatg tgcccttcgt
    22501 cggtgaaacg cagggcgttg ctcaacaggt tggtaatgac ctgacgaata cgctgcggat
    22561 cgcccattaa cgcagtcggt acatcgtcgg caattgccgt tgccaggcga atcgggcgac
    22621 ctttcacccg tccgctcatt aattgcaggg tactttccag caacgggcgc ggttcaaagg
    22681 gttcatcgct gaccgaaaca ttcttgccgc ctgcttcgat agccgaataa tcgagaatat
    22741 cgttgaggat ggtcagcaac gattcgccag agtcggtaat tgcccgcaaa tcatcacgct
    22801 gagcgttaag tgcggggtta tctgccagta gctgtgcggt accgagaata ccgtacagcg
    22861 gtgtgcggat ctcatggctc atcgccgcca gaaacgctga ttttgcctgg ttggcttttt
    22921 ctgcttccgc ccgtgcctgt cggtgttcta tcaccagttc ctgcaattca gctgtacgcg
    22981 ctttgacctg cgccgccaac tgttcacggt ggcgattcag tgcatgaaca ctgctgcgaa
    23041 acgcatccat cagccgcccg atggtgtcca gttcccgcac gccagcggtt tccgggaaag
    23101 gggagtcgat atcaccatcc agcaagcgtt gtagcgcctg cgtttgttct gcaagagggc
    23161 gcgtgactga gcgataaacc acgcgccaga ggatcagaat cagtgcgcaa agagaaacta
    23221 ttcccagcaa taacaggctg tattgcccgc gtgcactggc tttttccaga tgtgccagcc
    23281 cgtgctgatt acgcagctca atggtgtcga ccagctgact gacttcgcta ctaaactgcg
    23341 cgaattgggc gatgttattt tgggcgagag tttgcaggtg attgctgatt tcactgtcct
    23401 gctgatacag cgccagcaaa tcgctatatt ggctaacggt agttaacgtt gttgctacct
    23461 gagtacgaac acccggatct tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat
    23521 tattgagctg cttttccagc gttggcgcat ttttctggat ctgttccagc cccagattca
    23581 tcaccatttg ctgcacccgc agggcgctaa ggcgcagttc attcatctgg ttaacatact
    23641 caagatcgat atcaatcagc cgatcgagtg cactttctgc ggcctgacgc tgatgttgtt
    23701 cgatcaaatc gtaaatcccg gcctgggtcg ctccggcgga ggttgccgca ttattcgcct
    23761 gaccttgcgc caggcgtgcg atttcatcgg cggcggcgac tatctgctga ctgagttgct
    23821 gttgttgctg gcgaagttgc aaacgctgcc ccaccagttc cccttgctga cgtaacgaac
    23881 gggagatctc ctgctcctgt tgttcaatag cggtggtgtc aaaatcttgt tcccgtaacg
    23941 cttgcagcaa cgcattaatc ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc
    24001 acatcttttc gttatcggca ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg
    24061 cttcgctcaa ctggcgcgcc atattcatgg taggaatcaa tgcctgagtg ttgtcttttt
    24121 ccacctggct gataaagcgc aggttgtacc atcccaccag ggtactggtc agggttaaca
    24181 gcgccatcag ggcaaagccc atccagagtc ttcgggtcag ggttaaattc acggtcggtg
    24241 cactttgggt gaaaaaggtt gagtcgcaaa gcggaatgca tctagcataa agccttatta
    24301 ttgatgaggc tatcatgcgc gtactgctat tgttactact ttcccttttc atgttatcgg
    24361 cattttcggc tgataacctg ttgcgctggc atgatgcaca gcattacacg gtgcaagcct
    24421 ctacgccgct aaaagccaaa cgcgcatgga aactgtgcgc gctttatccc agtttgaaag
    24481 attcatactg gttatcgttg aactatggta tgcaggaggc tgctcgccgc tacggtgtgg
    24541 atttaaaagt gctggaggca ggcggctaca gccagttggc tacccagcaa gcacaaatcg
    24601 accagtgtaa acagtggggc gcagaggcca ttttgctcgg tagtagcacg acgtcatttc
    24661 ccgatctgca aaagcaggta gcaaatctgc cggtgatcga actggtaaat gctattgatg
    24721 ctccccaggt gaaaagccgc gttggtgtgc cctggtttca gatgggctat caacctgggc
    24781 gatatctggt gcaatgggcg cacggtaacc cactgaacgt gctgttgatg cccggacccg
    24841 ataacgccgg gggcagtaaa gagatggccg agggttttcg cgcagctatt gctggaagcc
    24901 cggtacgtat tgttgatatt gcgcttggcg ataacgatat tgaaatccag cgtaacctgt
    24961 tgcaggagat gctggagcgc catccagaaa tcgacgtcgt tgccggaacg gccattgcgg
    25021 cagaggcggc aatgggtgaa gggcgtaacc tgaaaacgcc gcttaccgtg gtgtcgtttt
    25081 atctttcaca tcaggtgtat cgcgggctga agcggggaag agtgattatg gctgccagcg
    25141 atcagatggt ctggcagggg gaactggcgg ttgagcaggc catcaggcaa ttacaggagc
    25201 aatcggtgtc tgataatgtc agcccaccga ttttagttct gacgccgaaa aatgccgacc
    25261 gcgaacatat ccgccgctcg ctgtcaccgg ggggatttcg tccggtctat ttttatcagc
    25321 acacatcagc ggctaagaaa taaccttcgc catgttgcgt caccagtaaa tccgcgctga
    25381 gtttatggcg taaacgacga attaacacat cgacggtgcg caagtcaggg ttttccaccc
    25441 gacgcgcaga gagcatacgc agcagacgtt cacggctaag aatttcgccc ggattcgtca
    25501 caaatgccac caacatttca tactctgcgc gggtcagttt aatcggctcg ccatcccgct
    25561 ccagcgtatg gcgcgacaca ttcaggcaat aaccggcaaa gcgatagcag ttgtcctgtg
    25621 tgtgcggttg agcttgtcgc gcgaggtcga ttcgccagag cagatttttc acccgtacta
    25681 ccagttcgcg cagttccagc ggtttggtga cgtaatcgtc tgcgcccatt tccagcccaa
    25741 caatacggtc aatccgatcg ctgcgtcccg taaccagaat aatccccacc gttgagcgtt
    25801 ctcgcagggc gcgggttaac atcaggccat tttcatcggg caaattaata tccagcagaa
    25861 ttaaatccac cggctgattc tgcataattt cccgcagccc ggcaccgctc gccgtaacgg
    25921 aaacggtata cccctcctga gtgaagtagg attgtaatcg cgcctgggta accggctcat
    25981 cttcaacaat aacaatgtga tgtggcatca gagggtttta ctcattctgt tcatatctgt
    26041 tcatattctg ccgtaagccg ttcatcctga ccagtgccgc tgttcatatt tactcattaa
    26101 gatcgcttca ctaaaccata attttacagg ggctattatg cggaaactct ggaacgcgct
    26161 acgccgaccc agtgctcgtt ggtcggtact ggcgctggtc gctattggga ttgtgattgg
    26221 cattgcgctg attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg
    26281 tgtcagttgc cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcacttcca
    26341 gaacgcctcc ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg
    26401 catggtgaag cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc
    26461 cattgataca cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc
    26521 gcgaatgaaa gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga
    26581 tcatgccaag cagcatcctg aagcggcgcg tcagatgaag gtggcagcga aagataatca
    26641 atcctgcatc gactgtcata aaggtattgc ccaccagtta ccggatatga gtagtggctt
    26701 ccgtaagcag ttcgatgagc tgcgcgccag tgctaatgat agtggtgaca cgctgtactc
    26761 cattgatatt aagccgattt atgcggcgaa aggcgataaa gaagcgtctg gttctctgct
    26821 gcctgcttcg gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaatcac
    26881 tggttggacg gaaagtgcag gacgtcagcg tgtactcacc caattcccag gtaaacgcat
    26941 ctttgttgcc tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac
    27001 cgttgccgac accaataccg agtggagcaa gttgcaggct actgcgtgga tgaagaaagg
    27061 cgacatggtg aacgatatca aaccgatctg ggcttatgcg gattcgctgt acaacggcac
    27121 ctgtaaccag tgccacggcg caccggaaat ctctcacttt gacgctaacg gttggatcgg
    27181 cacgctcaac ggcatgattg gctttaccag cctcgataaa cgtgaagaac gcaccttgtt
    27241 gaaatatctg caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga
    27301 agaaaaataa tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa
    27361 ctcggcggct taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca
    27421 actgcggcgc aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg
    27481 cactgggggg ctatccgcgc gacggtgaag gagggtcgct tcgtggcagc aaaaccgttc
    27541 gaactggata aatatccgtc gaaaatgatc gccggattgc cggatcatgt acacaacgcg
    27601 gcgcgtattc gttatccgat ggtacgcgtg gactggctac gtaagcgcca tctgagcgac
    27661 acctcccagc gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg
    27721 ttctatgaag aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc
    27781 agtggttggc aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagcgatt
    27841 gccttgcatg gtaatagcgt gggtacaggc ggagattact ctaccggggc tgcgcaggtg
    27901 atcctgccgc gcgtagtcgg ttcgatggaa gtgtatgaac agcaaacctc ctggccgctg
    27961 gtattgcaga acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa
    28021 gcgaactggt ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa
    28081 gtcgccgccg gtgaaattga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag
    28141 tatctggggc gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg
    28201 caactggctc tggcgcatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct
    28261 aactactgtg taggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag
    28321 ccgaaagatg ccgcatgggc tgaaaaactg acaggtatta atgccgaaac cattcgtggg
    28381 ctggcgcggc agatggcggc gaacagaacg cagattattg ctggctggtg cgtgcagcgt
    28441 atgcagcacg gtgaacagtg ggcgtggatg attgtcgttc tggcggcgat gttggggcaa
    28501 attggcctgc caggtggagg ttttggtttt ggctggcact ataacggcgc aggcacgccg
    28561 gggcgtaaag gcgttattct gagtggtttc tccggctcta cgtcgattcc gcctgttcac
    28621 gacaacagcg actacaaagg ctacagcagc accattccga ttgcccgttt tatcgatgcg
    28681 atcctcgaac cggggaaagt gatcaactgg aacggtaaat cggtaaaact accgccgctg
    28741 aaaatgtgta tttttgccgg aactaacccg ttccatcgcc atcagcagat caaccgcatt
    28801 attgaaggct ggcgcaagct ggaaacggtt atcgccatag ataaccagtg gacctcaacc
    28861 tgccgctttg ccgatatcgt gctgcctgcg accacgcagt ttgagcgtaa cgatctcgac
    28921 cagtacggca accactccaa tcgtggcatt atcgccatga aacaggtcgt gccgccgcag
    28981 ttcgaggcgc gcaacgactt cgatattttc cgcgagctgt gccgtcgctt taatcgcgaa
    29041 gaagccttta ccgaagggct ggacgaaatg ggctggctga aacgcatctg gcaggaaggt
    29101 gtacaacagg gcaaaggacg cggcgttcat ctgccagcgt ttgatgactt ctggaataat
    29161 aaagagtacg tcgagtttga ccatccgcag atgtttgttc gccaccaggc attccgcgaa
    29221 gatccggatc tcgaaccgct gggcacgccg agtggcctga ttgagatcta ctcgaaaacc
    29281 atcgctgata tgaactacga cgattgtcag gggcatccga tgtggtttga gaaaatcgaa
    29341 cgctcccacg gtgggcccgg ctcgcagaag tatccgttgc atctgcaatc tgtgcatccg
    29401 gatttccgac ttcactcgca gttatgtgag tcggaaacgc tgcgtcagca atatacggta
    29461 gcgggcaaag agccagtgtt cattaacccg caggatgcca gcgcgcgcgg tattcgtaac
    29521 ggtgatgtgg tacgcgtgtt taacgctcgc ggtcaggtgt tggcaggggc agtggtttct
    29581 gaccgctatg cacccggcgt ggcgcgaatt catgaagggg catggtacga tccagataaa
    29641 ggcggcgagc ctggtgcgct gtgcaaatac ggtaacccca acgtgttgac catcgacatc
    29701 ggtacttcgc agttggcgca ggcgaccagt gcgcatacca cgctggtgga aattgagaag
    29761 tacaacggaa cagtggagca ggtgacggcg tttaacggcc ctgttgagat ggtggcgcag
    29821 tgcgaatatg ttcccgcgtc gcaggtgaaa tcatgaccac gctgacagca caacagattg
    29881 cctgtgttta cgcctggctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    29941 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    30001 tcactgcggc ggtgaatgag ctggaaaacc gtattgccac tcagacagta cgtgacgatg
    30061 ctcgtctgga actggccgct gacttttgcg gcctgttttt gatgaccgac aaacaagctg
    30121 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    30181 aggcagggat ggaaaccagc ggcaatttca acgaaccggc agaccatctg gcgatctatc
    30241 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    30301 tcgacaatct gcggcaaaaa acactgacgg cgctgcgaca atggttacca gagtttgctg
    30361 cgcgttgcca tcagtacgac agtttcggtt tttacgcggc actaagccag ttattgctgg
    30421 tgttagtgga atgcgaccac caaaacagat aacgtcgttt gttggcctga taagacgcgt
    30481 ttagcgtcgc atcaggcatt agtgcgcagt tgccggatgc ggcgagaacg ccttatccgg
    30541 cctacgggaa cagtgctttt tgtgggcctg ataagatgcg ttaagcgtcg catcaggcat
    30601 tatggcgcag ttgccggatg cggcgagaac gccttatccg gcctacggga acagtgcttt
    30661 ttgtgggcct gataagacgc gttaagcgtc gcatcaggca acaaactcac gggtgagcca
    30721 caaaccggga aagccgctgg cgcagcaggc ggttttcctg cttcaggtgc gcaatatcat
    30781 ccattaacgt cagcgccacc gcgatccccg gccagtccag agccagttca tgacgcaggc
    30841 gtaccgcgcg ttgcaccaca atggcggcat ggtcgtcaaa tacccaggtt gtctcctgga
    30901 tctcccgcgg ttcaaccacc cccaaaccga caatttcatt caactcctct tcagagatgc
    30961 cggtatgcag gcaaaattcg gtaatagtaa aagtcaccgt aacattagcc attatgcttt
    31021 cccccaatct ttacgtggat caaaagacga ctgggcgtct gccagttgct gccacagcgc
    31081 ggcagtgttt tcatccggtt tcggcggcat cacgattttc agtaccgcat acagatcgcc
    31141 ggtctgtttt ttgctcacca gacctttgcc tttaacgcgc aatcgttgcc cggcctggct
    31201 gccaggcggg atagtcagca aaatgctttc tttcagtgtt ggaacggtga ctttagtacc
    31261 cagcgccgct tcccacgggc taaccggcac cactatttcc agatcctggc cgacaatatc
    31321 aaacagcgga tgtggcgcaa tgtgaatcac cagccacagg tcgccatttg gaccgccgtt
    31381 ttcgcccggc gtcccctggc ctttcaggcg gatgcgttga ccattgccga cgcccgccgg
    31441 gatcttcaca ttcagcgttt tcgggatttc ctgttcgatc atgccaaagg cgttataaac
    31501 cggcaggtta tagctgatgg tacgcttatg ctcagtaagc gtttcttcga ggaaaaccgc
    31561 cacttcgatt tcaatatcgt ggccgcgtgt ggcggggcgt tgacggctct ggcgggcatg
    31621 ctgaccgaaa attgacgaga agatatcgtc aaaatcttcg gcgttaaaac tctgaccgtc
    31681 gccatgatgg aactgacggt taaattgcgg atcgttgcga tgttgccaca tctgatcata
    31741 ctcagcgcga cgttgttcat cacttaacac ttcccaggct tcggcgacct ctttgaagcg
    31801 ggcttcggca tccggttctt tgctgacatc aggatggtat ttgcgggcca gtcgacgata
    31861 ggcggtcttg attgtcttga gatcgtccgt cggtttcacg cccatgatgg cgtaataatc
    31921 ctttaattcc atagcgttat ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg
    31981 taactcctat aagtgtaggg taatcctcaa aatttcatat gccaacacag aatatgttat
    32041 tgaaatcatc gcggagggga ggtcgcctca aggtgggagg aacatatttt atttacctcc
    32101 ctcctgaact tttggctcat gcttacaagt taaacaataa ggaaaggaat gcacaactcg
    32161 actatttaaa tcgtt
//
LOCUS       DAZUCC010000033        32115 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZUCC010000033 DAZUCC010000000
VERSION     DAZUCC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.033"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..478
                     /gene="agaB"
                     /locus_tag="ACHY09_001366"
     CDS             2..478
                     /gene="agaB"
                     /locus_tag="ACHY09_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFS3884359.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMSITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            517..1320
                     /gene="agaC"
                     /locus_tag="ACHY09_001367"
     CDS             517..1320
                     /gene="agaC"
                     /locus_tag="ACHY09_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFS3884360.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1310..2101
                     /gene="agaD"
                     /locus_tag="ACHY09_001368"
     CDS             1310..2101
                     /gene="agaD"
                     /locus_tag="ACHY09_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFS3884361.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPLLKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHIGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2102..2869
                     /gene="agaI"
                     /locus_tag="ACHY09_001369"
     CDS             2102..2869
                     /gene="agaI"
                     /locus_tag="ACHY09_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFS3884362.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(2919..3782)
                     /gene="rsmI"
                     /locus_tag="ACHY09_001370"
     CDS             complement(2919..3782)
                     /gene="rsmI"
                     /locus_tag="ACHY09_001370"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS3884363.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3847..5883
                     /gene="lpoA"
                     /locus_tag="ACHY09_001371"
     CDS             3847..5883
                     /gene="lpoA"
                     /locus_tag="ACHY09_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFS3884364.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSELTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            5841..6236
                     /gene="yraN"
                     /locus_tag="ACHY09_001372"
     CDS             5841..6236
                     /gene="yraN"
                     /locus_tag="ACHY09_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFS3884365.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6256..6846
                     /gene="diaA"
                     /locus_tag="ACHY09_001373"
     CDS             6256..6846
                     /gene="diaA"
                     /locus_tag="ACHY09_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFS3884366.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6856..7431
                     /gene="dolP"
                     /locus_tag="ACHY09_001374"
     CDS             6856..7431
                     /gene="dolP"
                     /locus_tag="ACHY09_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFS3884367.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7544..8584)
                     /gene="yraQ"
                     /locus_tag="ACHY09_001375"
     CDS             complement(7544..8584)
                     /gene="yraQ"
                     /locus_tag="ACHY09_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS3884368.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(8657..9292)
                     /gene="yraR"
                     /locus_tag="ACHY09_001376"
     CDS             complement(8657..9292)
                     /gene="yraR"
                     /locus_tag="ACHY09_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFS3884369.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9420..9938
                     /gene="yhbO"
                     /locus_tag="ACHY09_001377"
     CDS             9420..9938
                     /gene="yhbO"
                     /locus_tag="ACHY09_001377"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFS3884370.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9918..10361)
                     /gene="yhbP"
                     /locus_tag="ACHY09_001378"
     CDS             complement(9918..10361)
                     /gene="yhbP"
                     /locus_tag="ACHY09_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFS3884371.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10412..10714
                     /gene="yhbQ"
                     /locus_tag="ACHY09_001379"
     CDS             10412..10714
                     /gene="yhbQ"
                     /locus_tag="ACHY09_001379"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFS3884372.1"
                     /translation="MTPWFLYLIRTADDKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(10701..11204)
                     /gene="yhbS"
                     /locus_tag="ACHY09_001380"
     CDS             complement(10701..11204)
                     /gene="yhbS"
                     /locus_tag="ACHY09_001380"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3884373.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11198..11722)
                     /gene="ubiT"
                     /locus_tag="ACHY09_001381"
     CDS             complement(11198..11722)
                     /gene="ubiT"
                     /locus_tag="ACHY09_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFS3884374.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11931..12926
                     /gene="ubiU"
                     /locus_tag="ACHY09_001382"
     CDS             11931..12926
                     /gene="ubiU"
                     /locus_tag="ACHY09_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS3884375.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12935..13813
                     /gene="ubiV"
                     /locus_tag="ACHY09_001383"
     CDS             12935..13813
                     /gene="ubiV"
                     /locus_tag="ACHY09_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS3884376.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAILDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13894..14901
                     /gene="yhbW"
                     /locus_tag="ACHY09_001384"
     CDS             13894..14901
                     /gene="yhbW"
                     /locus_tag="ACHY09_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFS3884377.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15019..16263)
                     /gene="mtr"
                     /locus_tag="ACHY09_001385"
     CDS             complement(15019..16263)
                     /gene="mtr"
                     /locus_tag="ACHY09_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFS3884378.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(16417..18306)
                     /gene="deaD"
                     /locus_tag="ACHY09_001386"
     CDS             complement(16417..18306)
                     /gene="deaD"
                     /locus_tag="ACHY09_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFS3884379.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18299..18379)
                     /gene="yrbN"
                     /locus_tag="ACHY09_001387"
     CDS             complement(18299..18379)
                     /gene="yrbN"
                     /locus_tag="ACHY09_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFS3884380.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18486..19370)
                     /gene="nlpI"
                     /locus_tag="ACHY09_001388"
     CDS             complement(18486..19370)
                     /gene="nlpI"
                     /locus_tag="ACHY09_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFS3884381.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19479..21614)
                     /gene="pnp"
                     /locus_tag="ACHY09_001389"
     CDS             complement(19479..21614)
                     /gene="pnp"
                     /locus_tag="ACHY09_001389"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS3884382.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21861..22130)
                     /gene="rpsO"
                     /locus_tag="ACHY09_001390"
     CDS             complement(21861..22130)
                     /gene="rpsO"
                     /locus_tag="ACHY09_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS3884383.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22279..23223)
                     /gene="truB"
                     /locus_tag="ACHY09_001391"
     CDS             complement(22279..23223)
                     /gene="truB"
                     /locus_tag="ACHY09_001391"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS3884384.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23223..23624)
                     /gene="rbfA"
                     /locus_tag="ACHY09_001392"
     CDS             complement(23223..23624)
                     /gene="rbfA"
                     /locus_tag="ACHY09_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS3884385.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23788..26460)
                     /gene="infB"
                     /locus_tag="ACHY09_001393"
     CDS             complement(23788..26460)
                     /gene="infB"
                     /locus_tag="ACHY09_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS3884386.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(26485..27972)
                     /gene="nusA"
                     /locus_tag="ACHY09_001394"
     CDS             complement(26485..27972)
                     /gene="nusA"
                     /locus_tag="ACHY09_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS3884387.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(28000..28452)
                     /gene="rimP"
                     /locus_tag="ACHY09_001395"
     CDS             complement(28000..28452)
                     /gene="rimP"
                     /locus_tag="ACHY09_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS3884388.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(28659..28735)
                     /locus_tag="ACHY09_001396"
     tRNA            complement(28659..28735)
                     /locus_tag="ACHY09_001396"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28699..28701),aa:Met,seq:cat)
     gene            29083..30426
                     /gene="argG"
                     /locus_tag="ACHY09_001397"
     CDS             29083..30426
                     /gene="argG"
                     /locus_tag="ACHY09_001397"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFS3884389.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(30434..32059)
                     /gene="yhbX"
                     /locus_tag="ACHY09_001398"
     CDS             complement(30434..32059)
                     /gene="yhbX"
                     /locus_tag="ACHY09_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS3884390.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT         7750 a         8675 c         8043 g         7647 t
ORIGIN      
        1 aatgagcagt ccaaatattc tcttaacccg tattgataac cgtctggttc acggtcaggt
       61 tggcgtgacc tggacatcca ccatcggtgc gaatctgctg gtagtcgtgg atgatgttgt
      121 cgctaacgat gatattcaac agaaattaat gagtattacc gcagaaacct acggctttgg
      181 cattcgtttc tttactatcg aaaaaaccat taacgtcatc ggcaaagctg caccgcatca
      241 aaagatcttc ctgatttgcc gtacgccaca aacggtacgt aaattggtag aaggtggtat
      301 tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg gaagggaaaa agcaaatcag
      361 cagtaaagtt tatgtcgatg accaggatct cacggactta cgttttatta aacaacgtgg
      421 cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa gaacaaatcc ctgactaaat
      481 ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc atgaaataac cctacttcag
      541 ggattatccc tggcggcgtt agttttcgtt ctggggattg atttttggct ggaagcctta
      601 tttttattcc gcccgataat cgtttgtacc ctaactggcg ctattctcgg tgatattcag
      661 actggcttaa ttaccggtgg tctgacagag ttggctttcg ccggattaac ccctgcaggt
      721 ggtgttcagc cgcccaaccc gattatggcg ggtctgatga ccaccgtcat tgcatggtct
      781 acgggcgttg atgccaaaac agcaattggt cttggcctgc cgtttagttt gttaatgcag
      841 tatgtcattc tgttcttcta ttctgctttc tcattattta tgaccaaagc cgataaatgc
      901 gcgaaagaag cggatacggc agcgttttcc cgacttaact ggacaacgat gctcatcgtt
      961 gcttcagcgt atgcggtgat tgctttcctc tgtacttacc tggcacaagg ggcgatgcag
     1021 gcgctggtga aagcgatgcc cgcctggctg acccacggct ttgaagtggc tggcggtatt
     1081 ctgcctgccg ttggttttgg cttgctgctg cgcgtgatgt tcaaagcgca atatatccct
     1141 tacctgatcg ccggcttcct gtttgtttgc tacatccagg tcagcaacct gttgccggtt
     1201 gccgtactgg gcgcaggctt tgcggtgtat gagtttttca atgcgaaatc ccggcagcaa
     1261 gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg actacagcaa tgggatctga
     1321 aatcagtaaa aaagatatca cccgtctggg ctttcgttcg tcactgctac aagcaagctt
     1381 taactacgaa aggatgcagg cgggcggctt tacctgggcg atgttgccgc tcctgaaaaa
     1441 gatttataag gacgacaaac caggcctaag cgcggcgatg aaagataacc tagaatttat
     1501 taatacccac ccgaatctgg tcggattcct gatggggtta ttaatttcga tggaagagaa
     1561 aggggaaaac cgcgacacca ttaaaggcct caaagtggcg ctgtttggcc caatcgccgg
     1621 gattggcgat gcgattttct ggtttacctt gttaccaatt atggcgggga tttgctcatc
     1681 gtttgccagt cagggaaacc tgctggggcc gattctgttc tttgccgttt acctgcttat
     1741 ctttttcttg cgcgtcggct ggactcacat cggttactcg gtcggcgtga aggctattga
     1801 taaagtgcga gagaactcgc agatgattgc ccgttcggca accatcctcg ggatcacggt
     1861 aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg gtgacatcgt ttgccatcga
     1921 cagtacccac agcgtcgcgc tgcagcagga tttcttcgat aaagtcttcc cgaacatttt
     1981 accgatggcc tacaccctgc tgatgtatta cttcctgcgg gtgaaaaaag cgcatccggt
     2041 gctgttaatc ggcgtgacgt ttgttctttc tattgtttgt tctgcattcg gcattttgta
     2101 aatggaacga ggcaccgcgt ctggcggtgc ctctttactt aaggaatttc atcctgtgca
     2161 aacccttcaa caagttgaaa actatacggc gttaagtgaa cgtgccagcg aatatttatt
     2221 ggccgtgatc cgtagcaaac cggatgccgt gatttgcctg gcgaccggag ccacgccatt
     2281 actgacgtat cattatctgg tagaaaaaat ccaccagcag caggttgatg tcagccagct
     2341 caccttcgtg aagctcgacg aatgggtgga tctgccatta acgatgcctg gtacctgcga
     2401 aactttcctg caacagcata tcgtgcagcc gctggggcta cgtgaagacc agctcatcag
     2461 ctttcgctcc gaagagatta atgagacaga gtgcgaacgg gtaacgaacc tgattgcgcg
     2521 caaaggcggt ctggatttat gcgttctcgg gttggggaaa aacggtcatc ttgggctgaa
     2581 cgaaccggga gaaagcctgc aaccggcctg ccatatcagt caacttgatg ccagaacaca
     2641 gcaacatgag atgttaaaaa ccgcgggccg tcctgtgact cgtgggatca ccttaggcct
     2701 gaaggatatt ctcaatgccc gcgaagtttt gttactggtg actggcgaag gaaagcagga
     2761 tgcgacagaa cgttttctca cggctaaagt ctctaccgct atcccggctt catttttatg
     2821 gctgcataac tatttcacat gcatgattga tgagatgtgt cgtcgttaac tcgcctgcta
     2881 caggcgttgc aacgctattc ctgctgcgac gcctgtaatc actctccctg ctgctccagc
     2941 gcatacttat acagcgcatt tttcttcacg ccgtgaatct ctgccgccag cgccgccgct
     3001 tttttcagcg gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag
     3061 tcttcttcct gcgctttatg gccttcgaca atcagcacca tttcgccttt gcgacggttt
     3121 tcatcttctt tgacccacgc cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag
     3181 gttttggtca gctcacgcgc cagaaccacg tagcgggatt cgcctaatac cgcaacgata
     3241 tcttccaggc tatctaacag acggtgggta gattcataaa aaatcagcgt gcgcggctcc
     3301 gcttcaatag cttttagcgc atcacggcgg ccttttgatt tggcaggtaa aaagccttca
     3361 taacaaaaac gatcggatgg taaacccgcg gcgcttaacg cagtgatagc agcacacggc
     3421 cctggtagcg gcaccacgcg aatccccgct tcacgacagg tacgcaccag atggtagcca
     3481 ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg caatgttttg cccctcttgc
     3541 agtttcgcca gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac
     3601 cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt gacgcgtatc ctcggcggca
     3661 atcagatcaa cggcctgtaa tacctctaat gcacgctggg tgatatcagc cagattgccg
     3721 attggcgtcg gtacaatgta aagctggccc tgagaattat ccgccgattg gtgttgtttc
     3781 attgtgtcgt ccgtattgcc gatttaatat tgagcattgc gtaaaaaaaa tatcactgga
     3841 tacattatgg taccctcaac attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt
     3901 ctggcagccc tgattttcgc cggttgtggc acccatactc ccgatcagtc cactgcttat
     3961 atgcagggca cggcgcaggc tgattctgcc ttttacctgc aacagatgca gcaaagttct
     4021 gatgatacca ggatcaactg gcaattactc gccattcgtg cactggtgaa agaaggtaaa
     4081 accggacagg cggtggagtt gtttaaccaa ctaccgcaag aactgaacga ttctcagcgt
     4141 cgcgagaaaa cactgctggc ggcagagatt aaactggcgc agaaagattt tgctggcgcg
     4201 caaaacttgc tggcgaaaat cacacctgcc gatttagaac aaaaccagca agcccgttac
     4261 tggcaggcaa aaatcgatgc cagccagggg cgtccttcca ttgatttact gcgcgcgtta
     4321 attgctcagg aaccgctgct cggtgcgaaa gaaaagcagc agaatattga cgccacatgg
     4381 caggcgctct cctccatgac tcaggagcag gcgaatacgc tggtgatcaa cgccgacgaa
     4441 aatattctgc aaggctggct ggatttgcag cgcgtctggt ttgataaccg taacgatcct
     4501 gacatgatga aagctgggat cgccgactgg cagaaacgtt atccgaacaa cccgggcgcg
     4561 aaaatgctac caacgcagtt ggttaacgta aaagcgttta aacccgcatc gaccaacaaa
     4621 atcgccctgc tgttgccgct gaatggccag gcagcggtat ttggtcgcac tattcagcaa
     4681 ggctttgaag cggcgaaaaa tatcggcact cagccagtgg cggcccaggt agcagccgca
     4741 cctgccgcag acgtagcaga acaacctcag ccgcaaaccg tggatggcgt tgccagcccg
     4801 gcacaagctt cggttagcga gctgaccggt gaacagcctg cggcccagcc ggtacctgta
     4861 agcgccccgg cgacaagcac cgcagcggta agcgcacccg caaatccatc cgcagagctg
     4921 aaaatctacg atacctcatc acaaccactt agccagatct taagccaggt tcagcaggat
     4981 ggtgcgagta ttgtggtcgg tccgttgctg aaaaataacg ttgaagagtt gctgaagagc
     5041 aacaccccgc tgaacgtgct ggcactgaac cagccggaga acatcgaaaa ccgcgtcaat
     5101 atttgttact tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg tcatattcgt
     5161 gaccagggta aacaagcgcc gctggtgctg atcccacgca gttcattggg cgaccgcgta
     5221 gccaatgcgt ttgcgcaaga gtggcagaaa ctgggcggcg gcaccgttct gcaacaaaaa
     5281 tttggttcca ccagcgaatt acgcgcgggt gttaacggcg gttctggtat tgctttaacg
     5341 ggtagcccga ttactcccag agcgacaacc gactccggca tgacgaccaa caatccaacg
     5401 ctgcaaacca cgccaaccga tgaccagttc accaataatg gcggtcgtgt cgatgcggtg
     5461 tacattgtgg caacgccggg tgaaatcgct tttatcaaac cgatgatcgc catgcgtaac
     5521 ggtagccaga gcggtgcaac gctgtacgcc agttcccgca gcgcacaagg caccgcaggc
     5581 ccggatttcc gtctggagat ggaaggcttg cagtatagcg aaatcccgat gctggcaggc
     5641 ggtaatctgc cgttaatgca gcaggcgctc agcgcggtga acaacgatta ttcgctggct
     5701 cgcatgtatg cgatgggcgt cgatgcctgg tcgttggcaa atcatttctc acaaatgcgc
     5761 caggttcagg gttttgaaat caacggtaat accggaagtc tgacggctaa cccggattgc
     5821 gtgattaaca ggaagttatc atggctacag taccaacaag gtcaggtagt ccccgccagt
     5881 taaccaccaa acagaccggc gatgtgtggg aagcacaagc gcgtcgctgg ctggaaggca
     5941 aaggactgcg gtttatcgcc gctaacgtga acgagcgtgg cggcgagatc gatctgataa
     6001 tgcgtgaagg ccggatcacc gtttttgtcg aggtgcgtta ccgccgctct gcgctttatg
     6061 gcggcgcggc agccagtgtg acccgcagca aacaacacaa attattacag actgcccgct
     6121 tgtggctcgc gcgtcataat gggagttttg atactgtgga ttgccggttc gatgtggtag
     6181 ccttcaccgg gaatgaggtt gagtggatta aggatgcctt taatgaccac tcataattaa
     6241 ggtttaagga ttagcgtgca agaaagaatt aaagcttgct tcactgaaag cattcaaact
     6301 caaattgcgg cggcagaggc gcttccggat gccatctcgc gtgcagccat gacgctggtt
     6361 cagtctctgc tcaatggcaa caaaatcctc tgttgtggta atggaacttc cgctgccaat
     6421 gcacagcatt ttgctgccag catgatcaac cgtttcgaaa cggagcggcc cagcttacct
     6481 gccattgcac taaatactga taatgttgtc ttaacggcga ttgccaacga tcgcttacat
     6541 gatgaagtgt atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc
     6601 atttccaccc gtggcaacag ccgcgatatt gtaaaagcgg tagaagctgc cgttacgcgt
     6661 gatatgacca ttgtggcatt aaccggctat gacggcggtg aacttgcagg tttgttaggg
     6721 ccacaggatg tggagatccg cattccttcg catcgtagtg ctcgcattca ggaaatgcat
     6781 atgctgacgg taaattgcct gtgcgatctg atcgataaca cgcttttccc tcaccaggat
     6841 gtttaaggag aatacatgaa ggcattatcg ccaatcgcag tccttctttc cgcgctgctg
     6901 ttgcaaggtt gtgttgccgc tgccgtagtg ggtaccgctg ctgtaggtac caaagccgca
     6961 actgacccac gcagtgtcgg cacccaggtg gacgatggta ccctggaagt gcgcgtgaac
     7021 agcgcattgt cgaaagacga acaaattaag aaagaagcgc gcattaatgt cacggcctat
     7081 cagggcaaag tgctgctggt tgggcagtca ccaaacgctg aactttcggc tcgcgccaaa
     7141 cagattgcta tgggcgtaga cggtgccaac gaagtgtata acgagattcg tcagggccag
     7201 ccgattggtc tgggcgaagc gtctaacgat acgtggatca ccaccaaagt acgttcgcag
     7261 ctgttaacca gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa
     7321 gtgttcctga tggggctggt gactgaacgt gaagcgaaag cggcggcaga tattgccagc
     7381 cgggtaagcg gcgtgaagcg ggtaactacg gcgtttacgt ttattaaata gcagcccttg
     7441 taatgcctga tgcgacgctt gcagcgtctt atcaggccta caacgatgca aaccgtaggt
     7501 cggataaggc gtttacgccg catccgacat gttttccctc aaatcaaacc aacagcgcca
     7561 gcccgccgac aatcacacca gacactgcca ccatcgcccc cgtcagccat aaggctttcg
     7621 ccgggaacgc tttgcgcagc ataatcagtg acggcaaact caccgccggg agcgtcatca
     7681 acaatgccag cgccggagca gttcccatac ctgccagcat catcgtttgt acaatcggaa
     7741 tttctgctgc cgtgggaatg acaaacaagc atcccgctac cgccatcgcc accacccaca
     7801 tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcga gcggcaccca
     7861 acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagcgtc catagcgccc
     7921 tgccccagcg gctaaaaaat cctccctgtg cttccggtat gtcaatttcg accggtgcct
     7981 gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc aacaccatca
     8041 ccagccccgc caccagacga atcgccgcaa aaccccagcc gaggacgaac cccataaaca
     8101 ccagcgtcgc cgggtttaac aacggattcc ccatccagaa tgccagcgca ccgcccatcg
     8161 acacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta cacatcatgc
     8221 ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
     8281 gcaataacca gtcacgcggg atcagcacct gaatcaacga cccgagcagt acgcccagca
     8341 ccgccgcttt ccataccgcg aggaaataga tcatcgcgta atccaacgcc gcctgccacg
     8401 gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gcagcagtaa
     8461 acgctttgcc gtagtaaggt tgccatttca cataccagag accagcaatg acaacgagaa
     8521 agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa gactgaccag
     8581 tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
     8641 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca
     8701 caccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat
     8761 caatggattt ccagttaccc ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca
     8821 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
     8881 agttctgggc gattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
     8941 agtgggcatt agcacccata gcactgacca ccagcatatg ctgcgcaccg agccgccgcc
     9001 cggttaatgc ggtatccacc accagcgtgt aatcggcatg aataaacgcc tctttactcc
     9061 ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatggga tcggtgacct
     9121 gcgccagcgc gtcggtcagt tgcggatcat ggggattaaa cacaccgggc atatcgccca
     9181 acggacgtcg cgtcggcgcg gctatggcgt taactttcgg ttcattaatc aacatccgca
     9241 gcaggtgacc acccaccagc cccgttgcgc ctgtaatcag tacctggctc atcttcgctc
     9301 ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccaggcttaa tagtctacga
     9361 ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca
     9421 tgagtaagaa aattgccgtt ttaatcactg atgaatttga ggattcagaa tttacttcac
     9481 ccgcagacga gttccgtaaa gccggacacg aagtgattac cattgaaaaa caagcgggta
     9541 aaacggtgaa aggcaaaaaa ggagaagcca gcgtggccat cgataaatcc atcgatgaag
     9601 tgacgccagc ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc
     9661 gtggcgacaa ccgttttgtc actttcaccc gtgattttgt gaatagcggc aaacctgttt
     9721 tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac
     9781 tgactgccgt taaaccgatc attattgatg ttaaaaacgc gggcgcggaa ttttacgatc
     9841 aggaagtggt ggtcgataaa gatcagctgg ttaccagccg tacaccggac gatctgccag
     9901 cgtttaaccg cgaggcatta cgcctgctcg gtgcctgagt tacgtagcca gatcattttt
     9961 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
    10021 ggggccgaca gcattctggc aaccggaaag cggcgattgt acgcctggcg cgcaaggtcg
    10081 ctttcctcac cttcaagcct gcggatctcg cctttaaatt gcacgccgcg aattaacgct
    10141 accgttttcg gctgaccgtt taccgttccg gcaactgccg cctgcggccc actcatctgc
    10201 gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaaaaga
    10261 taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt gacaacatgt
    10321 tgttttgcca gccaacggct gatggcggtg agtgtttcca ttgctgttct cttttatact
    10381 gtgggcctga ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg
    10441 taccgccgac gataagcttt ataccgggat caccacagat gtcgaacgcc gctatcagca
    10501 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactaa cgctggcgtt
    10561 ttccgcgcca gtgggcgatc gttcgctggc actacgggcg gaatatcgcg ttaagcaact
    10621 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag aactgttgag
    10681 cagcctgcaa accccaaaga ttaaaagcga ttgaagtgct cgtgatactc aaccagacca
    10741 gttacaccgt tcagcgcgtc atccgctaaa cgatgtacct ggaaggcact ttcagtgccc
    10801 ggccagcggc aacgcagatc gtgatgtgcc gccagttcaa agccgaaacg gctgtacagc
    10861 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
    10921 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatctac cgccagcggt
    10981 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
    11041 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
    11101 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgaacgacg cagcagggca
    11161 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
    11221 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
    11281 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
    11341 catacagccc cagctccgta tcgccttcaa ttaccagccg acgctggaag aagagcgtat
    11401 ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca ctaaaactca
    11461 catcagcttg cgcgttctgg ctaacgacca gtttgccatt caccaccgag gtaaaccatt
    11521 gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
    11581 catcatccaa cgcctggcgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
    11641 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
    11701 gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
    11761 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
    11821 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
    11881 cagttcgtta acatttttgc gctttgatag cgcaaccttc aggaaaaatt atggagctgc
    11941 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
    12001 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
    12061 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg
    12121 cgattaacac ttttgcgcat ccggacggtt acgcccgctg gcagcgcgcc gtggatatgg
    12181 cggcgcagtt gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gagtacgccg
    12241 ccgagcgtta cccgcatatt gagcgtcatg tgtcagtgca ggcttcggcg accaatgaag
    12301 aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt
    12361 tgtcgattca tcaggtgaaa caactggcac gggtcacacc tgtcccgctg gaagtgtttg
    12421 ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg
    12481 gtgagtcgcc caacaccgtg ggcgcgtgtt ctccggcccg tttcgtgcgc tggcagcaaa
    12541 cgccgcaggg actggaatct cgcctgaacg aagtgctgat cgaccgttat caggatggcg
    12601 aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc
    12661 acgcgctgga agaacccacc agtctcaata ccctggaact gctgccggag ttaatggcgg
    12721 cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    12781 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa aacttcgtac
    12841 cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa actacccttg
    12901 gcgcgtatca ccgtaaatgg cagtgagaaa agcaatgaaa tattccttag ggccggtgct
    12961 atggtactgg ccaaaagaga cgctggaaga cttttatcag caggccgccg ccagcagcgc
    13021 agacgtgatt tatcttggtg aagcggtctg tagcaaacgc cgggcaacca aagttggcga
    13081 ctggctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc tctccacgct
    13141 ggcgctggtg caggcgtcct ccgaacttgg cgaactgaaa cgttatgttg agaacggtga
    13201 gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc
    13261 gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acactgaaaa tattgctcaa
    13321 acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc tggtgaatct
    13381 gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag tcctgagcta
    13441 cggtcatctg ccgctggcct actccgcccg ctgctttacc gcgcgttcgg aagaccgccc
    13501 gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg tgctatcgca
    13561 ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct acgtttacaa
    13621 cctcggtaac gagctggcat ccatgcaggg gctggtcgat gtggtccgcc tgtcaccgca
    13681 gggtactgac actttcgcga tactcgacgc cttccgcgcc aatgaaaatg gcgctgcacc
    13741 actgccgttg acggcaaaca gcgattgtaa cggctactgg cggcggctgg ccgggctgga
    13801 gctgcaagcc taagtaaata gctcactttg ttaacaactt taactactct ttaatgcagt
    13861 attaaagatt aatcagtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg
    13921 ctactcgatc tggcccccat tcccgaaggc tcttcagcgc gggaagcgtt ctcccactct
    13981 ctcgacctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcggaacac
    14041 cacaatatga ccggcattgc cagtgctgca acatcagttt tgattggcta tctggcggcg
    14101 aataccacta cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccattg
    14161 gtgatcgccg aacagttcgg cacgcttaat acgctctatc cggggcgaat cgatttgggg
    14221 ctgggtcgtg cgccgggtag tgaccagcga accatgatgg cactacgtcg tcatatgagc
    14281 ggtgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc
    14341 gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg
    14401 ttaggctcca gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gtttgcgttt
    14461 gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagcaacttc
    14521 aaaccgtcgg cacgactgga aaaaccatac gcgatggtgt gcatcaatat tatcgctgcc
    14581 gacagcaacc gcgacgctga atttctgttt acctcaatgc agcaagcctt tgtgaaacta
    14641 cgccgtggcg aaaccggaca actaccgcca ccgattcaaa atatggatca gttctggtca
    14701 ccgtctgagc agtatggcgt gcagcaggcg ctgagtatgt cgttggtagg tgataaagcg
    14761 aaagtgcgtc atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtt
    14821 aacgggcaga ttttcgacca ccaggcacgc ctgcattcgt ttgagctggc gatggatgtg
    14881 aaggaagagt tgttggggta gtgtgtttta acgtaggaag ccttatccga gatggcaacg
    14941 ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt
    15001 cggataaggc accgctgatt actgatacac cggcagcaaa ttaaagctcg ataagatatg
    15061 gaccagtgcg ttgccaacgc caaataccag aatcagcata atcatcggct tgccgcccca
    15121 gacgcggaat ttcgggctac caaagcgttt acgcgatgcg cgggccaaca gcgccggaac
    15181 aattgccgcc cagatggtag ccgctaagcc agcataacca atggcgtaca ggaatccgtt
    15241 aggaaacagc aggccaccca caacaggcgg ggcaaaggtc agcaacgctg ttttcaaacg
    15301 gcccatagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt
    15361 tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaagatccag
    15421 gctacggctg ttcagcacgc cgcttaatgc ctgtaccagc acatcaatat taccgccctt
    15481 ctcggcgatg ccgataaact ccggacgagg gatgttgccc atcgtcgcca gcaaccagat
    15541 ggtatacagc gccagcgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg
    15601 atctttgccg taatacttca tcaggctcgg cacgttaccg tgataaccaa acgatgccag
    15661 acagaatggc agtgtcatca gcagatacgg cgcataagac gcattgcttt cggcgacgtt
    15721 gaacaaggtc gttggctgta catgccccag caggctgccg aaggtgagga agaaagtaat
    15781 gactttcgcc cccagcacaa ttgctgtcat ccggctgaca gctttcgtac tcaaccacac
    15841 cacgaacgct accagcagtg caaaaccaaa tccagctgcc cgtgcaggga cgttcagcga
    15901 catctccgcg aaggtgtgat gcagaatcga accgctggca gaaatatagg cgtaagtcag
    15961 gatatagagc acaaaggcaa tggaaatgcc gttgactacg ttccagcctt tgcccagcaa
    16021 atctttggtg atggtgtcaa agctagaacc gattcgataa ttcaggttag cttccagaat
    16081 catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgccgcca ttgaccagaa
    16141 gaaccacgcc ccggacatga ccacaggcag agaaaacatc cctgcgccaa taatggtgcc
    16201 gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc
    16261 catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac
    16321 cagtacacga gtacaaaaga cagaaaaaaa gccccgatgg taaaaatcgg ggctgtatat
    16381 attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg
    16441 acgaccggta gagtcatcac gacgcggagc gcggccttca cgacgttcgc cgctaaaacg
    16501 acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg
    16561 acgttcgcca ctgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc
    16621 atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg
    16681 cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt
    16741 gatgttacca atgtaacggc tgctgatgtc gccttcgtta gcaatcgcac caacgatatg
    16801 acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca tatcgccaac
    16861 atcacgacgt tcacgacgcg gacgatcttc acggtcacca cgcgggccac ggtcgttacg
    16921 atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc
    16981 tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgcgg cggccagagt
    17041 ttcgagatcc agctcttcac cttctgccgt cggctgaatt ttgctcagca gtgcacggta
    17101 ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg
    17161 acgtttgccc agcagttctg cgttcggcag ttcaacttcc ggaatggtca gcttcatggt
    17221 gcgttcaatg ttacgcagca gacgacgctc acggttctca acgaacagca gcgcacggcc
    17281 agcacggccc gcacgaccag tacgaccgat acggtgaacg taagactcag aatccatcgg
    17341 gatatcgtag ttaactacca ggctgatacg ctcaacgtcc aggccacggg ccgcaacgtc
    17401 ggtcgcaatc aggatgtcca gacgaccatc tttcagtcgt tccagtgtct gctcacgcag
    17461 cgcctggttc atgtcaccgt tcagcgcggc gctgttgtag ccgttacgct caagggcttc
    17521 tgccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc
    17581 cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccagacag tccagtagct
    17641 ctggctgatg tcaggacggg tagtcacgct ggactgaatg cgcacttcct gcggctcttt
    17701 cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt
    17761 ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg
    17821 cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc
    17881 acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg
    17941 cagggcgcgt aattgcacgt cataacgttg gccgccgtac agggcaacca cgtttacgcc
    18001 gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt
    18061 cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct gcagcagagg
    18121 taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc
    18181 attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc
    18241 gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc
    18301 agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa
    18361 attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac
    18421 aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac
    18481 gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat
    18541 tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg
    18601 aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc taaatagaag
    18661 ttggtttcac tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt
    18721 tccattaacg tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac
    18781 tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg
    18841 agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga
    18901 taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc
    18961 ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct
    19021 tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaatact
    19081 tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc
    19141 agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg
    19201 gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa
    19261 gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct
    19321 gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc
    19381 cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt
    19441 taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc
    19501 ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg
    19561 atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg
    19621 taatcggtca ctttctcaac gcgtttgtca gcgatctgag agatgtgtac cagaccttct
    19681 ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccattg
    19741 tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc
    19801 gctttctcgc cgtcggtcgc tgcgattttc acagtaccgt catcttcgat ttcgatggtg
    19861 gtgccagttt cttcggtcag agcacggata acagaaccgc ctttaccgat aacgtctttg
    19921 atcttgtccg ggttgatctt gatggtatgg atacgcggag cgaactcaga gatatcgccg
    19981 cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc
    20041 tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc
    20101 aatgcagaga taccgtcacg ggaacccgca actttgaagt ccatatcgcc caggtgatct
    20161 tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt
    20221 gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg
    20281 ccgcacacgg aagccataga agaggaaccg ttggattcgg tgatttcaga cacaacacgt
    20341 acggtatacg ggaatttgtc catatccggc atgactgcca gcacgccgcg cttcgccaga
    20401 cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacagag
    20461 tacggaggga agttgtagtg gaacaggaag gtgtcagtac gttcgcccat cagttcatca
    20521 agaacctgcg cgtcacgtgc agtacctagc gttgcggtaa ccagcgcctg agtttcacca
    20581 cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacac cagtacgcac atccagacca
    20641 cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga
    20701 acaacatttt tctcgatagc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg
    20761 tcttcagcaa gcagtgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc
    20821 tcttgtttgt cggtgatgcg gtaagcatcg ctcagacgag cttcagccag tgcagcaacg
    20881 cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgcgg tttaccggct
    20941 tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg accgaagact
    21001 actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagtaca
    21061 gcggcttcag tacccgcaac aaccagatcc agtttgctct ctttcaactc gtcctgagtc
    21121 gggttcagta cgtactggtc attgatgtaa cctacacggg cagcaccaat cgggccattg
    21181 aacggaatac cagacagaga cagggctgcg gaagcaccaa tcatcgcgac gatatccggg
    21241 ttaacctgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct
    21301 tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg
    21361 cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc
    21421 tcctgatagt taacggtcag cgggaagaag tcctgacctg gtttggcttt tttctgacca
    21481 acaacggtaa cgaataccgc agtgtcatcc atgctaacca taacagcggc agtagcctga
    21541 cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg
    21601 atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg
    21661 ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt
    21721 gggtaaagcc tctcattagc cgcgcgaacc tctgcagcgg aagatcattc atagcaacaa
    21781 tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc
    21841 ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa
    21901 cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga aaccatgcgc
    21961 agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg
    22021 ttgatctgtg cagtcagcag tgctacctga acttcggtag aaccggtgtc gtttgcgtca
    22081 cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat tttaaaactc
    22141 caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt gtacgttacg
    22201 caaagtgtta aacaatttac gcgacgttaa gcggcagtat tctactcgta gcgacttgtt
    22261 atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag gcgcaacgcg
    22321 gccttcatcg tcaatttcgc ccataccgat aaacttgccg ttctcaccct ctgtaacgcg
    22381 aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt tgaagtaaac
    22441 agaagacgtt aacggaagat tcaccactgg atagtccgaa gctggactgt ccattggcat
    22501 cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt gctcaaccag
    22561 ttcacgcaga tgttccaggg tcaccatccg ttcaaccgga tatttactta ccgccagacg
    22621 acgcaagtaa ataacatgcg cgccacaacc gagtttttcg ccgagatcgt caatgatagt
    22681 gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc cttcatggcg
    22741 aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggaactt caatgccctg
    22801 acgcgcgtat tcgtacagtt ttttgccctg atatttcagt gctgaataca tcgaagggat
    22861 ctgttcgata tcgccacgga aggtatccag cgccgccgcc agttgctctg cactaaaggt
    22921 taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac gctgtccaag
    22981 acgcgcaatg acccgataac gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc
    23041 ttccccgagg caaattggca acatgccggt cgccagcggg tccagcgcac cggtatgccc
    23101 ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcgttgc tggacatccc
    23161 ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact
    23221 cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg tttgaccacg
    23281 ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta gaagaaggtc
    23341 agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat gaaaccagaa
    23401 gcttcctgca gcgctttgat gcccgcttta accgcgtctt catctttgtc gttgaggaac
    23461 gttacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga aacggtggtc
    23521 atcatgccca gacgaggatc tttaatttca cgctgcagga tgagagcgat ctctttttgc
    23581 atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa ttctcctgac
    23641 aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc tgttcacgtt
    23701 ggcctgataa gacgcgccag cgtcgcatca ggcaatccat gccggatgcg gcgtaaacgc
    23761 cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggattt cgatgatttc
    23821 gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc cgataccaca
    23881 ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg actccagctc
    23941 gccttcgtag ataaccacgt tgtcgcgcag aacgcggatc gggttgtgac gtttaaccac
    24001 accttcggta accatacaac ctgcgatggc accaaatttc ggtgatttga atacgtcacg
    24061 aacttccgcc agaccgataa tctgttgttt cagttccgga gacagcatac cgctcattgc
    24121 cgctttcact tcgtcgatca ggttatagat gacggagtag taacgcagat ccaggctttc
    24181 cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa ccaggatggc
    24241 attggacgcc gcagccaggg tagcgtcggt ttcggtgata ccacctacgc cagaaccgat
    24301 gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga tagcttccac
    24361 agaaccctgt acgtctgcct tcaggacgat attcacttcg tgaacttcgc cttcggtcat
    24421 gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa cttcgcggaa
    24481 tttaccctga cgatagagtg caacttcacg cgctttcttc tcgtcacgta caacggtaac
    24541 ttcatcaccc gcagccggaa cgccggacag gccgaggatt tccaccggaa tggacggacc
    24601 cgcttccagc acttcctgac ccagttcgtt gcgcatcgca cgaacacgac cgtattcgaa
    24661 gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa cggtagcaac
    24721 cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca tacctttacg
    24781 taccgctttc agttccagaa cttccgcctg cagcaggata gcgtccagca gctcatcgat
    24841 accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc actcttccgg
    24901 caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt ctggtttatc
    24961 gatcttgttc actgcaacca ccaccggaac ctgcgccgct ttcgcgtgct ggattgcttc
    25021 gatggtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga tgtccgttgc
    25081 ctgcgcacca cgagcacgca ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt
    25141 gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgggtaa tgccgcccgc
    25201 ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt taccgtggtc
    25261 aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgctgcac ccgtgtcacg
    25321 gtcgctcatt accgcttctt ccaactcgtt ttcacgacgc aggataactt tgtggcccat
    25381 ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg ttgccattgc
    25441 gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct tgttcgccag
    25501 ttcgccaacg gtgatggttt cgccgatcac aacgtcacgg ttaacggcct gagcaggctt
    25561 ctggaagcct tgctgcagcg aagaaccttt acgttttccg cccttaccgc cacgtactgc
    25621 tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt tcttcggacg
    25681 cgcagctttc gcgttacgac cacggccacg gccgccttcg acttcacgat cgctttcgtc
    25741 ttctgcctgg cgagcatgtt gagaagtagt aacgtgataa tcgctggaat cttcagtcgg
    25801 ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt cagcaacgcg
    25861 acgtgcttct tcttcgagtt tacgacgcgc ttcttcttcg gctttacgct tgagctctgc
    25921 agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat cgtcttgttg
    25981 attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag cttcacgctt
    26041 agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcacgtttgg ccgattcttc
    26101 tgcctcacga cgggcttgct cttccgcttc acgctgcgct tgctcttccg cagcaaggcg
    26161 ttcagcctct tgcggatcgc gtttcacaaa ggtgcgtttc ttgcggactt cgatttgtac
    26221 cgatttgctt tttccaccgg taccaggaat gttaagggtg ctgcgtgttt tacgttgcag
    26281 cgtcaatttg tccgggcctg aatttttctg attcaggtgg tcaatcaaag tctgtttctc
    26341 ttgtgcagac acagagtcgt cagcagactt ccggatacct gcatcagcaa attgctgtac
    26401 caggcgttcc acggaggtct gtcgctctgc ggccagcgtt ttaatcgtta catctgtcat
    26461 gctgttcctt cctgctacag tttattacgc ttcgtcaccg aaccagcaaa tattacgggc
    26521 agccataatc agtgctccgg ctttttcgtc ggtcaaccct tcgatatcag ccagatcatc
    26581 aatgccctgt tcggcgagat cttccagcgt acaaacgcca cgggcggcca gtttgaatgc
    26641 caaatcacga tctatccctt caaggttcag cagatcgtca gccggtttgt tatcaccgag
    26701 gctttcttcc tgggcctgtg caatggtggc cagtgcattt ttagcacgct cgcgcagtgc
    26761 ttcaacggtc ggctcatcaa ggccttcgat ttccaacagc tctttcatcg gcacataggc
    26821 cagttcttcc agcgtcgaga agccttcttc taccagaaca gtcgcgaagt cttcgtcgat
    26881 gtcgagatat ttggtgaagg tgtcgatcgc tgcgtgcgct tccgcctgat gcttagcctg
    26941 caggtcgtca acggtcatca cgttgagttc ccaaccgctc agctgcgaag ccagacgcac
    27001 gttctgacca ttacggccaa tcgcctgcgc caggttaccg gcttcaacgg cgatatccat
    27061 ggtgtgttta tcttcatcca ccacgataga agcaacgtct gccggtgcca ttgcgttaat
    27121 cacgaactgc gccgggttat catcccacag gacgatatcg atacgctcgc cgcccagttc
    27181 agtagaaacc gcctgaacac gcgcgccacg catacctacg caagcaccta ccggatcgat
    27241 acgtttatcg ttggttttca ccgcgatttt cgcacgagaa cccggatcgc gagccgctgc
    27301 tttaatttca atcacttctt cgccgatttc cggcacttca atgcggaaca gttcgatcag
    27361 catttccggc ttggaacggg tgacgaacag ttgcgcgcca cgcgcttccg ggcgaacgga
    27421 atagagcacg ccacgaacgc ggtcgccagg acggaagttt tcacgcggca gcatatcttc
    27481 gcgcaggatc acggcttcag cgttgttgcc cagatccaga gagatgttgt cgcggtttac
    27541 ttttttcacc acgccggtga tgatttcacc ttcgtgttca cggaactgat caaccaccat
    27601 cgcacgttcg gcttcacgca ctttctgcac gataacctgt tttgccgtct gggtggtgat
    27661 acggtcaaag gtaacagact caatctgatc ttcaacgtaa tcgcccaggt tcaggctttc
    27721 atcttcataa cgtgcggctt caagggtgat ttccttggtc ggctgggtga cttcatcaac
    27781 aactaaccag cgacggaagg tgtcaaaatc accgcttttg cgatcgatct gtacacggac
    27841 gtcgatctct tgctcatatt ttttctttgt tgctgtcgcc agcgcgcttt ccaatgcttc
    27901 gaaaatcttc tcgcgaggta gcgccttttc attggatacg gcttcaacta cagccaaaat
    27961 ttctttgttc atcgcgggct tttcacctca tccagactat taaaagtggg gaaccaggtt
    28021 cgccttctgg atattactca gcgcgaacac ttcatcttta ccttcgacgg taactgtgat
    28081 catttcaccg tctaccgctt tgataacgcc ctgccattta cgacggtttt gtaccgccat
    28141 gcggagaacc agagtcacct cttctccgac gaaacgggcg tagtgttcag ccgtgaacag
    28201 tgggcgatcg agacccggtg aggagacttc caggttataa gcaacggtga tgggatcttc
    28261 aacatccagc acagcactta cctggtggct cacatcagca caatcatcaa cattgatgcc
    28321 atcttcacta tcaatataga tgcgcagtgt ggatgtgcga ccgcgaataa attcgatgcc
    28381 aaccagttca aaacccaggg cctcaactgg cgcagtaatc atctctgtta atttttgctc
    28441 taatgtggac aagcccaccc ccaagacata aaaaaagggc ctaaagccca gttattctgt
    28501 agtcagataa caaaaaaccc cgataaatcg gggctttata taactgaacc ctataaccgc
    28561 aactgcggtc tggagcactt tccagaagga ttttttcaaa tcccactacg aaggccgaag
    28621 tcttcacagt atatttgaaa aaggactcta agggaaagtg gttgcggggg ccggatttga
    28681 accgacgacc ttcgggttat gagcccgacg agctaccagg ctgctccacc ccgcgcctga
    28741 aacgtggcaa attctactcg ttttgggtaa aaaatgcaaa tactgctggg atttggtgta
    28801 ccgagacggg acgtaaaatc tgcaggcatt atagtgatcc acgccacatt ttgtcaacgt
    28861 ttattgctaa tcacgtgaat gaatatccag ttcactttca tttgttgaat acttttgcct
    28921 tctcctgctc tcccttaagc gcattatttt acaaaaaaca cactaaactc ttcctgtctc
    28981 cgataaaaga tgattaaatg aaaactcatt tattttgcat aaaaattcag tgagagcgga
    29041 aatccaggct cctcatcagt taattaagca gggtgttatt ttatgacgac gattctcaag
    29101 catctcccgg taggtcaacg tattggtatc gctttttccg gcggtctgga caccagtgcc
    29161 gcactgctgt ggatgcgaca aaagggagct gttccttatg catatactgc aaatctgggc
    29221 cagccagacg aagaggatta tgatgcgatc cctcgtcgtg ccatggaata cggcgcggag
    29281 aacgcacgtc tgatcgactg ccgcaaacaa ctggtggccg aaggtattgc cgctattcag
    29341 tgtggcgcat ttcataacac cactggcggc ctgacctatt tcaacacgac gccgctgggc
    29401 cgcgccgtga ctggcaccat gctggttgct gctatgaaag aagatggcgt gaatatctgg
    29461 ggtgacggta gcacctataa aggaaacgat atcgaacgtt tctatcgtta cggtctgctg
    29521 accaatgctg aactgcagat ttacaaaccg tggcttgata ctgactttat tgatgaactg
    29581 ggcggtcgtc atgaaatgtc tgaatttatg attgcctgcg gtttcgacta caaaatgtct
    29641 gtcgaaaaag cctactccac ggactccaac atgctcggcg caacgcatga agcgaaggat
    29701 ctggaatacc tcaactccag cgtcaaaatc gtcaacccga ttatgggcgt gaagttctgg
    29761 gatgagagcg tgaagatccc ggcagaagaa gtcacagtac gctttgagca aggtcatccg
    29821 gtggcgctga acggtaaaac ctttagcgac gacgtagaaa tgatgctgga agctaaccgc
    29881 atcggcggtc gtcacggcct gggcatgagc gaccagattg aaaaccgtat catcgaagca
    29941 aaaagccgcg gcatttatga agcccctggg atggcactgc tgcacattgc gtatgaacgt
    30001 ttgttgaccg gtattcacaa cgaagacacc attgagcagt atcacgcgca tggtcgtcag
    30061 ttgggccgtc tgctgtacca gggacgttgg tttgactccc aggcgctgat gctgcgggac
    30121 tctctgcaac gctgggttgc cagccagatc actggtgaag ttactctgga gctgcgccgc
    30181 gggaacgatt attcaatcct gaataccgtc tcagagaacc tgacctacaa gccagagcgt
    30241 ctgacgatgg aaaaaggcga ctcggtgttc tcgccagatg atcgtatcgg tcaattgacc
    30301 atgcgtaacc tggatatcac cgatacccgc gagaaacttt tcggttatgc caaaactggc
    30361 ctcctctcct cctctgccgc ttcaggcgtg ccgcaggtgg agaatctgga aaataaaggc
    30421 cagtaattcg acatcaaccc tgcccttcag gggtggggtt accttgctta tcctctgtaa
    30481 actcctttct gagcatcaca taatcgaaaa catcatggtt tgcatccaca acccgtgagt
    30541 ctcctttata gtgtgcaatc acttcctcca gcgttttcgg ttgttccggc tttgttaccc
    30601 ccatccacgc attaatcagg taattattgt aagctgtgga aaagatgttg ttttccgttg
    30661 tgcgatccac gccatcccca agaacggggc tataccagat aaacatcggg acatgatatg
    30721 cctgctggct ggcctccctg cctccatgaa aatagacgtt cttcttcgta gggtcacgtt
    30781 ccaggccgtg gtcggcaaaa tacatgaccg aggcgcggcg atcttttaat aattcaaaaa
    30841 cctgacctag caaactatct gtgtaatgaa tggagttgtc atagcaggca tcctgatcgt
    30901 cctgcggttg aaacacggcg ctggattgcg gataggcgct acaagccggt tcatggcttc
    30961 catttaaatg aagaacaatc agtttctttt gctgcgtatt ttgctgtaat gcttgcgata
    31021 aatgcggcaa caacaattca tcaaatcctc tgacataaac tgtttccatg gcgcgcatgg
    31081 cgatactggt aactgctgta ccattctgcc gaaaagcgga ttgcgagctt agccagaaag
    31141 tctgaaatcc tgcttgatta gccatattaa taatgttgtc cgggtaatta tgaatgtcat
    31201 gactcaaaac ggaatcagca gttaaagaaa ggggaaccga cagcgcagtg taaggtgcac
    31261 cgcttattgc ttgattaaac agtttgatct gttttctttg tgcttcgact tgcggtgtcg
    31321 tagagcgtgt atatccatac aaagacatat tgtcgacgcg tacagactcc cccacaatca
    31381 acacgtaggt atcaatacct gtatccctga ctgataattg aaaatacggc accgtgtttg
    31441 caattgagag taatctttga tgctctttcg ctgctaaagc aaaataattg agattgaaaa
    31501 acggtgtata ggtagcaaat cgcgacgcta gtatatatgg actgaacgct tttttatttt
    31561 ttgcatcttt ataagcaaat tgacaagcgg aaaataaact gcccgaaatg acaatcagca
    31621 ataatattcc tgtcactttt ttcgtcggca aggaaacatc atattttatt attactgcca
    31681 aaaaaagaag ggataaaaag gcaaaagcac atagataagg gatatacatc cccagcattt
    31741 tgacaacttc atccggatca ctctgcagca cactaatcgc aaatccatca ttgaaagttg
    31801 taccaaaggt acaccatgaa tagaggctaa tactcatgtc tgccgctgtc agtaacacaa
    31861 atggtgcggc aacaagcagg cgaagcggaa gggatgatat acgtttaaca accaggatgg
    31921 tcagaacgaa gaacagcaat cgctgaacca tatgcgctcc ggaagaggcg actaagttcg
    31981 taataccaaa aacaataaca caaagataca acaaccaatg agatttaaaa cttctaacaa
    32041 atttgttgaa tactgtcatc cagaaatcca agttcacaac caatataaac aattatcaac
    32101 aatgttcaat tttat
//
LOCUS       DAZUCC010000034        30883 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZUCC010000034 DAZUCC010000000
VERSION     DAZUCC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.034"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..695)
                     /locus_tag="ACHY09_001399"
     CDS             complement(<1..695)
                     /locus_tag="ACHY09_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS3884391.1"
                     /translation="MQTTRPPYSVSFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAASGDISF
                     QTINDYGALTPRI"
     gene            complement(1043..1309)
                     /locus_tag="ACHY09_001400"
                     /pseudo
     CDS             complement(1043..1309)
                     /locus_tag="ACHY09_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1585..2901)
                     /gene="hipA"
                     /locus_tag="ACHY09_001401"
     CDS             complement(1585..2901)
                     /gene="hipA"
                     /locus_tag="ACHY09_001401"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFS3884392.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY"
     gene            complement(2901..3167)
                     /gene="hipB"
                     /locus_tag="ACHY09_001402"
     CDS             complement(2901..3167)
                     /gene="hipB"
                     /locus_tag="ACHY09_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFS3884393.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(3360..5249)
                     /locus_tag="ACHY09_001403"
                     /pseudo
     CDS             complement(3360..5249)
                     /locus_tag="ACHY09_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            complement(5399..6991)
                     /gene="lsrK"
                     /locus_tag="ACHY09_001404"
     CDS             complement(5399..6991)
                     /gene="lsrK"
                     /locus_tag="ACHY09_001404"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFS3884394.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDPEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVNELKELHNNTFENKVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLIDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(7070..8023)
                     /gene="lsrR"
                     /locus_tag="ACHY09_001405"
     CDS             complement(7070..8023)
                     /gene="lsrR"
                     /locus_tag="ACHY09_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFS3884395.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            8272..9807
                     /gene="lsrA"
                     /locus_tag="ACHY09_001406"
     CDS             8272..9807
                     /gene="lsrA"
                     /locus_tag="ACHY09_001406"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFS3884396.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSIQKMNNLLVALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFRRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSTGD
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            9801..10829
                     /gene="lsrC"
                     /locus_tag="ACHY09_001407"
     CDS             9801..10829
                     /gene="lsrC"
                     /locus_tag="ACHY09_001407"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFS3884397.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYVTGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTVLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            10829..11821
                     /gene="lsrD"
                     /locus_tag="ACHY09_001408"
     CDS             10829..11821
                     /gene="lsrD"
                     /locus_tag="ACHY09_001408"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFS3884398.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            11833..12855
                     /gene="lsrB"
                     /locus_tag="ACHY09_001409"
     CDS             11833..12855
                     /gene="lsrB"
                     /locus_tag="ACHY09_001409"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFS3884399.1"
                     /translation="MTLHRFKKIALLSALGIAVISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            12882..13757
                     /gene="lsrF"
                     /locus_tag="ACHY09_001410"
     CDS             12882..13757
                     /gene="lsrF"
                     /locus_tag="ACHY09_001410"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFS3884400.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKAGIPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            13781..14071
                     /gene="lsrG"
                     /locus_tag="ACHY09_001411"
     CDS             13781..14071
                     /gene="lsrG"
                     /locus_tag="ACHY09_001411"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFS3884401.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            14128..14886
                     /gene="tam"
                     /locus_tag="ACHY09_001412"
     CDS             14128..14886
                     /gene="tam"
                     /locus_tag="ACHY09_001412"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFS3884402.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STTLLHQRWPAARITGIDSSPAMIAEARSTLPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQSIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(14890..15804)
                     /gene="yneE"
                     /locus_tag="ACHY09_001413"
     CDS             complement(14890..15804)
                     /gene="yneE"
                     /locus_tag="ACHY09_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFS3884403.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISVVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(16001..17452)
                     /gene="uxaB"
                     /locus_tag="ACHY09_001414"
     CDS             complement(16001..17452)
                     /gene="uxaB"
                     /locus_tag="ACHY09_001414"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS3884404.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTGLVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILKKGMREAVRPLC"
     gene            complement(17680..19098)
                     /locus_tag="ACHY09_001415"
     CDS             complement(17680..19098)
                     /locus_tag="ACHY09_001415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS3884405.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(19237..19596)
                     /gene="yneG"
                     /locus_tag="ACHY09_001416"
     CDS             complement(19237..19596)
                     /gene="yneG"
                     /locus_tag="ACHY09_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFS3884406.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(19596..20522)
                     /gene="glsB"
                     /locus_tag="ACHY09_001417"
     CDS             complement(19596..20522)
                     /gene="glsB"
                     /locus_tag="ACHY09_001417"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFS3884407.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRMGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCIELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(20586..21974)
                     /gene="sad"
                     /locus_tag="ACHY09_001418"
     CDS             complement(20586..21974)
                     /gene="sad"
                     /locus_tag="ACHY09_001418"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFS3884408.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRVRSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNNGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETRARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            22075..22950
                     /gene="yneJ"
                     /locus_tag="ACHY09_001419"
     CDS             22075..22950
                     /gene="yneJ"
                     /locus_tag="ACHY09_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3884409.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAATKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPAMLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            23034..23153
                     /gene="yneK"
                     /locus_tag="ACHY09_001420"
     CDS             23034..23153
                     /gene="yneK"
                     /locus_tag="ACHY09_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFS3884410.1"
                     /translation="MVTPVSISNFISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            23295..24485
                     /gene="ydeA"
                     /locus_tag="ACHY09_001421"
     CDS             23295..24485
                     /gene="ydeA"
                     /locus_tag="ACHY09_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFS3884411.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGELITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(24510..25175)
                     /gene="marC"
                     /locus_tag="ACHY09_001422"
     CDS             complement(24510..25175)
                     /gene="marC"
                     /locus_tag="ACHY09_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFS3884412.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            25387..25821
                     /gene="marR"
                     /locus_tag="ACHY09_001423"
     CDS             25387..25821
                     /gene="marR"
                     /locus_tag="ACHY09_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFS3884413.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            25842..26225
                     /gene="marA"
                     /locus_tag="ACHY09_001424"
     CDS             25842..26225
                     /gene="marA"
                     /locus_tag="ACHY09_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFS3884414.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            26257..26475
                     /gene="marB"
                     /locus_tag="ACHY09_001425"
     CDS             26257..26475
                     /gene="marB"
                     /locus_tag="ACHY09_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFS3884415.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(26506..27405)
                     /gene="eamA"
                     /locus_tag="ACHY09_001426"
     CDS             complement(26506..27405)
                     /gene="eamA"
                     /locus_tag="ACHY09_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFS3884416.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            27600..28787
                     /gene="ydeE"
                     /locus_tag="ACHY09_001427"
     CDS             27600..28787
                     /gene="ydeE"
                     /locus_tag="ACHY09_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFS3884417.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYNLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRNEKIIATETGSAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            28914..29009
                     /gene="mgtS"
                     /locus_tag="ACHY09_001428"
     CDS             28914..29009
                     /gene="mgtS"
                     /locus_tag="ACHY09_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFS3884418.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            29009..29113
                     /gene="mgtT"
                     /locus_tag="ACHY09_001429"
     CDS             29009..29113
                     /gene="mgtT"
                     /locus_tag="ACHY09_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFS3884419.1"
                     /translation="MNGDNPSPNRPLVTTVYKGPDFYDGEKKPPVNRR"
     gene            complement(29228..30118)
                     /gene="dgcZ"
                     /locus_tag="ACHY09_001430"
     CDS             complement(29228..30118)
                     /gene="dgcZ"
                     /locus_tag="ACHY09_001430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFS3884420.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYELCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRVGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(30373..30765)
                     /gene="ydeI"
                     /locus_tag="ACHY09_001431"
     CDS             complement(30373..30765)
                     /gene="ydeI"
                     /locus_tag="ACHY09_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFS3884421.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         7764 a         7804 c         7381 g         7934 t
ORIGIN      
        1 ttaatacgcg gagttaatgc accgtaatca ttgatcgtct gaaaactgat atctccggat
       61 gcggcatgcg ggatatccac cgatacttca ccttttggcg gcaccatggt gtttgggaga
      121 ttgctatttc ctgctttcat attcgttacg gtaatgaagt agggcgttgg attaataaga
      181 gtaagtttgc taccgctgcg acgaaaccga agcattgcag gcgcttcttc aggtgccatt
      241 gccaggttcg tggggcgata aaacattttt atgcggctaa taattgccag ctgtaatgta
      301 ttctcattct tctggtcttt ttccatagca ggaatagctt taacattcac ccagaacagg
      361 ctttcacggt cgcctggtaa ctgatgatta gtggcgttga ttatccgcag cgtgttctct
      421 tttttacctt gcatggaaaa aagcggtggc gtaatgacaa actgggtgtc tttttggtca
      481 cccgcatttt ctatccatga ctgaatgaga tagacactgg caggatcatt attcgtgaca
      541 ggcaataaaa cctgtttctg gtttgccgga taaattaccc gcgtcgcacc cagtgcgaca
      601 gctgcgttgg caacggtgct atggcaagca aagagcaata aaagtaacac tgtcgccata
      661 aaggaaacag agtagggggg acgcgtagtt tgcatctgat gcattctcca tgtgttaaga
      721 ggataagcgg ggaaaccgtt acatcagaca atcggtaaaa tatctttaaa ttacaaggac
      781 acctaatcac aacaagttta ttgcagcact tgttgtagag agtatttgca aagacatttt
      841 atgtcgctaa ataacatgac gatttcatta tgcatgttat ttataaaccg tgactaaaca
      901 gaatattatt gcgaacaagg gaagtgtcag gaaaggtcaa gagcgaccaa tggtttatat
      961 ggctataaat cattagccat agttaatgtt tgcctggtgt aactaacacg gaattgctct
     1021 aaacagataa gtgtagggtt tattaaagct gaccgacaga ctttcttaac ctggtaatat
     1081 ctctcttagg tgactctcca aacatccgcg aatattcccg actgaaatgg gataagcttt
     1141 cgtaaccgac agcataggct gcagtggtga catcgtaatg ttcattcagc atcagtcgcc
     1201 tggcttcatt gaggcttagc catttccgat accggagtgg actcattccc gcaagctgtc
     1261 tgaaatgttg atggaaagag ggggtgttca tttgcacgcg gtttgccagc ctgactcttc
     1321 aacgctgaga agatgcggca aacggcaaac ctgcatacca gccagcctat atccggtatg
     1381 cacaggccaa ttatgaaaga ggagaggctt tatgttctgg aaccttgaag taataacgtc
     1441 gaccggaaaa tcactctgca tcatcactca gattcaattt aacataatat acattatgcg
     1501 caccaacata aaccaaggga gaatccagcc gttggcggtc atgattgtcc tgctcattaa
     1561 caacggccac accccatatc ttacttaata ccattctcgg cttaaccgac ggtgcaacct
     1621 caacacattg gtttcaactg ccgtcaccac gttctccgga aaatctgtcg gtaatgaagt
     1681 cttcacgtta tccagtgctg ccggaatcat tctggcaaag tcactcagga tttcatgcat
     1741 ctgcacttgc ggaaatttca gcacctttgc tgtcgccaga aaatgtcgcg gataaatttt
     1801 atcgattgcc gtttttttgc ctttggatgc gttaagcccc attgccagtt tgagatcgct
     1861 gatgtgtatt cctgcaccac ccaataccgg aaatgctgaa atgatgtcgt aaaatggcgt
     1921 gagtcgataa ctgccgccag cctgaataaa tacggagaag ttttttgcat ggccatccgt
     1981 tgcgccaatc aaccactgga acacctgaaa cttcataaaa tcatagcgat ctttcagcgc
     2041 ctcgctggac cccatcaaaa aagccatgat ccgcgcgatg cctgggcctc catctgattc
     2101 atatttcacc gatgaaggta aaccgaatgt ctgacacata tcctcctgtg gcaagcgaag
     2161 taaaaccgtt cgctcagcat tccaacgcct gtcaaaacgt tcgaccgcta acgcgcgcac
     2221 atttcccgct ttaatgattt ctgcgtccgg cacattcaac ccaagttctt tagccagcag
     2281 cagacagtaa tactcattat caacgctttg gctgagatcg agcgtcgcat tgggctgcct
     2341 gatttcgcca atcggtaatt taatgatgtg cgtcgtcggc gttattcctt tcggaatgca
     2401 ccagtcattg cctattctga gcagcgctgt cttctcctgc gcgccagcaa ccgagatgcg
     2461 aaagtcattt tcttctctaa tcatgcccag agggatatct gctttataag ccgttaatac
     2521 ttcttcaagc ctggcttcag tgagctgttc ccatgccatt atcgggcgcg ttatggtttc
     2581 attttcgggt aataacgtta cggcaccaac gctgtctcgc cctatttctg acaataaatc
     2641 aaacggttgt ctggatttag catgataacg tttaacgatc cgatcacgta caatcgggct
     2701 atcgggtaac aggttatcga agaagttaaa tacggcatca gaggtgatat tccccctctg
     2761 caatggcagc gaaagtgaca acggtctggc ataacggctg gctaaccact ccggtgcata
     2821 cttaaaggtg tgcgcgccat tggctaactt cgttaactcg cctacccgct ggttgttcat
     2881 ccaagtgaca agtttaggca ttaccactcc agatcttgct gttctgttga ttctggcgag
     2941 gcatttttcg tgtcgcatag cgtcattgag agttcaagcg actgtaaaat cttaaaaaag
     3001 gtcgtcagcg tggtattgtc agggttgttt tcgaaattgg aaatcgtcgc ctgcttaata
     3061 ccaatttttt tcgccagctc gctctgcgtc cagccatttt gctggcgaac cagtttcatt
     3121 gcattcgcca attgcgttgg gctatagatc ttctgaaagc tcatcatgtc atacgtccac
     3181 agcaaattta tccgcttaag gggatattat cagttttatc ctttagtgag gataagtcaa
     3241 tctggaaaca catcttatcc gctctacggg ataaacactg tgatatccgc gatcgcggat
     3301 aaaaaggagg atttaaaagt cgtttgaagt cggcaatatt aagccgcatg catcttcgac
     3361 atgcggctat tcccttgggt atcgcctttt acgttcatct tatcggtggg cgaattatcg
     3421 ccgcccagcg tcgcgttgaa cgtcatcagg ccattgtttc cggtatagtt accgttaacg
     3481 gtcagtacat tccccggttt ccccccttcg ctgttcattt gaaccgtgcc accattctcc
     3541 aggttaccgc cgacagtggt tttagcgaca cgcagtgtcc cccctggcat aacgttcaca
     3601 tcacctgcag tggtgacatt tcccgacatc acaccgctgt tatggatatt aatgtgctga
     3661 cttgccatat taatggcaga gtcggaaccg aaagcaattt caccacccac gatatccgtc
     3721 gtacccttat acgccgcgga atctttgtta agcgtcagga tcccggtacc cagttttgtc
     3781 acatcaccgc taccgctgat actgtgcgtc atcacccagc tacgctcagt ggagatggtg
     3841 agcaggccat tattgataac ggaagcatca cccaggtttt tctgctcgtt gacgctaagc
     3901 gcgctgcctg tatcaatgtt gaattgcccc acaaagcggc tattatcgcc atccagctct
     3961 acgtctgtcc tggcggttgc gctcacgata cccttcccgc ttatgctatt acgcagttcg
     4021 ccggtcacat tttttagcgt taacagaccg tcattggcaa tatttcctct gcctaacccc
     4081 tgcgtattat cgaggctgac ttccgcattt gggctaatgc tggttaacgc attgtagcgt
     4141 gccttaagcc cctcgatagc cagtgttccc cctgtaacat tcaggtttcc gccacctgtc
     4201 agttcgccct gactgatccc acctttattc accgtcagcg acccctcttt aaacaaaaca
     4261 gtcgaaccca tttgcccggt gaatgtctct accgtctggg tcgatccatt aagatcgacg
     4321 atggccgcgt tgctgatgtt cagttcccgg gtgttgccca gcgcgccatc cgcatcggta
     4381 cgtaatgtcc ccatctgaac gtaggttgcc ccctgatagt cgttctgacc gttggaaagc
     4441 gaaacctgtc gtaccgtatt gattgccaga tccccttcgc caccgatttt tgccgacata
     4501 tcgaccgttg cgccataagc tccgccatgt tcggctaacg tcagcttttg cccaccatgg
     4561 atgttcagcg ctttcagccc atagttaaca tacaaaccat caccaggtgc ggtcgtgagg
     4621 cggaatccat aatcaccctc ggcgaccacc gttccgtttt gcgcaatatg taacgttttg
     4681 tccgcttcca cctcgtcgcc ctgtaaatca cgtaacgtta atgaaccacc cgatccaata
     4741 accgtttgcg ccttcaccag ttgaacgccg acatggctat cgtcgtgttc catcaaattg
     4801 agcgtcgtca gagggttatt cgccatggga tcattccacg gtttaggcac gtcgataagc
     4861 acgtcgccca tcccgtttac ctgatagtta cggcctttcc aggtgtagtc acccgcgccg
     4921 acaaccagcg tatcgacgct gatatatccc tccgcaagcg tcgcagcagg tatatccgta
     4981 tcgaacgtga gcgtaccgcc attcatggct agtccaccaa tggattgctc tcccactttt
     5041 accgatgtgg tattttcact gtcagactgc agcatcgcgt aagtaagcgc agcggtgttg
     5101 tcgcgttcca gtgcgaaggt actggctttc agttgggcaa caccggcgaa ttcagtccct
     5161 gctgcatggg taaagccaaa cattttgtcg gaggatgaca gctgcactcg catcaagcca
     5221 tcgcctttga gcgcattgat cagcgtgaat agcaatttcc ccttgaatat agtactggtg
     5281 atggaacgat gaatctgcaa gctggcattt aacaagccag ctctaaaaga agggaaatga
     5341 gaataccaat actcaaaaac taacagccac ggtcatcatg gtgtggctgt caatgaaact
     5401 ataacccagg cgctttccat aacgacgtcg tcagtccatg atcaaccagc cccagctgat
     5461 cctgataaac tgcctgccac ttatcgcgtg aatcctgata aagttcatgc ttttccgggt
     5521 ctggtgtgtg cgtccgttcc caacgaacca agcgttctcc ggtttctgcc attgatgaaa
     5581 aaattccggc accgacgcca gctgcaatgg cacatcctaa tgcagtggct tctttgacca
     5641 ccggaatatt gacgggtaat cccgagacat cagcgagaat ttgactccat aatttccctt
     5701 ttgaacctcc gccagcaaaa accagcgagg tgggatgaat attcgagaaa tcagcaattt
     5761 gctgtaagtt acacgctgat acaatcgccg cattttcttc cagcgcacgg aacaatgtcg
     5821 ctttgttaca tttatccggg tcaatggaca agttaataaa ggaaggcgca gcgtgatacc
     5881 aggttttaaa gcgcattctg tcggagaata tcggcattac gccccacgac ccaggcggca
     5941 cccgactggc catctcttcc agcagcgtat aggtgtcgat gcctaaacgt tcggcaatca
     6001 gtttttcttc ggcacagaaa gcatcgcgga accagcgcat ggtgagtccg gtaaaaaagc
     6061 ttatagattc agcttgtacc atgccaggaa taacatgggg attaacgcgc acgttcattt
     6121 ctgggtctgt caccggcgcg gctaagttta caacttgctg ccagaatgtg ccgccaagaa
     6181 ccgcggtttg tgccggacgt acaacgccta accccaggca accaagctgc acgtcacctc
     6241 ctccaacgac caccggagtg cccgccttca gaccgcagag ttccgccgct tgtgaactta
     6301 ccacacccag caatgtgccg gtttctttga caggagaaag aatatcggca cgcaggccag
     6361 ccatatccag caatgcaggt ttccagtcac gggtggttag atcaataagt cccgtggtgc
     6421 cagcgttaga gggatccacc gcaagttcgc cgctgagcat ataggccagc cagtcgctga
     6481 tcatggtgat ggttgatgcc tgacggtaaa tatcggaacg atggtgcgcc agccaaagta
     6541 atctggggat ggcgcttaaa gccagcgttt gcccggtcgc tcgataaact ttgttttcaa
     6601 aggtattgtt gtgcagttct ttaagttcgt taacttcgcg cgccgctctg gcatccacat
     6661 tggcgcaggc ccagatcggt gttccttcat tgttatataa aacaatgcct tcacgcatcg
     6721 aacatgccga aacagcagcg atatactccg gggctatgcc ggcgttgtgc agcgcctggc
     6781 gcatacactc acacgccagt tgccagtttt tgttgagatc aaattccata gaaccaggaa
     6841 cgtccggtac tgccagatgc cgccactccg cctgtcccac ggctatttga ttgccttccg
     6901 ggtcgaatat cacagcccga atacttccgg tgcctgcatc cagcgccatc aggtagtact
     6961 ttgattctga aggggtaaag agtcgagcca ttagattatc ctcggcttat gcgttctgtc
     7021 tgttcctcta tacgttctcc atcattcccg gtaacaaggt catgcaaatt taactacgta
     7081 aaatcgccgc tgctgtgtcc tgatcggtaa ccagtgcgtt gatatagccg cctttcattg
     7141 cagcggcaat ggcttcggct ttattttctc cccctgccac gccaacccgg acgggtatgg
     7201 tcttcagcgc gcttaaaggt aagccaatca gttcgttatg tattttaata tccgtgacaa
     7261 cgtcaccttt tgcatcaaaa aagtagccta aaatgtcgcc aacagcccct tttcgaccaa
     7321 tcattaactg ttcgccctgg ctgatatagc cggagcgaat gattgttgcg tcgtcttgtt
     7381 gactcacagc cccaatgccg acaatcgcca catccgctgc ttgcgcggct agcagaacat
     7441 ctttgacgca attttcattt tttagcgtac gggcaatgtc agcggaggat gcccgcaacg
     7501 gagccggaat aatattcaca ctgcacgccg cgttaagctg cccgataccc gtcatataag
     7561 aaccgactcc accggagagc gtgaccaggc gaatttgctg tgacgaaata aaaccactta
     7621 agcgttgcag cgtattcata gttgcctcgc caaaaccaat cgccagcatc tgttgtggtt
     7681 gaagtaaact catcaacata tgcgccgcgc ctatccctag tcgcccgccg acatcagcat
     7741 ccgcaagccc tgggatcacc cggacatgtt gcagcgaaaa ctgacgacgt aattgtgttt
     7801 catattccag acagccttca aagcgagaat taatctgtac gcgaataatg ccggattgat
     7861 gccctttctc cagtaatcgc gacactttca aacgggtcag gccgagacga tcgctgatct
     7921 cactctgggt cagcccgtcg tggtagtaaa accacgcaat ccgcgcgacc tgttcttctt
     7981 cacacattcc ctgttctgaa attaccgaat cgttgattgt cataattcat tcttcacttt
     8041 gaacatattt aaatctttaa tgcaattgtt cagttcttgc gcatttatat ctgtgatagc
     8101 aaccacagtt tgactctacg agcatgaaca aacgcaaccg tgaaaatcaa aatagcataa
     8161 attgtgatct attcgtcgga aatatgtgca atgtccacct aaggttatga acaaattaaa
     8221 agcagaaata catttgttca aaactcacct gcaaaactga acgggggaaa tatgcaaacg
     8281 agtgataccc gcgcgttacc gctactttgc gcccgctcgg tttataaaca gtattcaggg
     8341 gttaatgtcc tgaaaggcat cgattttacg ttgcatcagg gggaggtcca cgccctgctc
     8401 ggcggcaatg gtgccggtaa atcgacgtta atgaagatta ttgccggtat tacccctgct
     8461 gatagcggta cgctggagat tgggggcaat aattacacca gattaacgcc agttcatgct
     8521 catcagctgg gcatttatct cgttccccag gaaccgctgc tttttccaag cctgtcgata
     8581 aaagaaaaca tcctgtttgg tctggcaaaa aaacaactct ccatacagaa aatgaataac
     8641 ttgctggtgg cgctgggctg ccagtttgat ctgcatagtc tggcaggatc gctggatgtc
     8701 gccgatcgcc aaatggtgga aatcctccgc ggcctgatgc gcgactcgcg gattctgatc
     8761 ctcgatgaac ctacagcctc gcttacccct gcggaaaccg aacgtttgtt tcgtcgcttg
     8821 caagagctgc ttgctactgg cgtgggtatt gtttttatct cgcataagct gccggaaatt
     8881 cgccagattg ccgatcgaat tagcgtgatg cgcgacggaa ccatcgcctt aagcggcaaa
     8941 accagcgaac tgtctaccga cgacattatt caggccatca cgccagcgct acgggaaaaa
     9001 tcgctctctg ccagccaaaa attatggctg gaattacctg gcaaccgccc acaacatgcc
     9061 gcgggaacgc cggtgctgac actggaaaat ctgaccggcg aaggtttcag gaatgtcagc
     9121 ctgacgctca atgccggaga aattctgggc ttggctgggc tggtgggagc cggacgcaca
     9181 gaactggccg agacgctcta tggtctgcgt actttgcgtg gcggacgcat tatgctgaat
     9241 ggtaaagaga tcaatagatt atccaccgga gatcgtttac tgcgcggtct ggtttatctg
     9301 ccggaagatc gccagtcatc cggacttaat ctcgatgctt cactggcgtg gaacgtctgc
     9361 gcccttactc ataaccttcg tggattctgg gcgaaaaccg cgaaagataa tgccaccctg
     9421 gaacgatatc gccgggcgct gaatattaaa tttaaccaac cggaacaagc tgcgcggacc
     9481 ttatccggcg gcaaccagca aaaaatcctg attgccaaat gtctggaagc ttcgccgcaa
     9541 gttttgattg tcgatgagcc gacgcgcggc gtggatgtct cggcgcgtaa tgatatctac
     9601 caactgttgc gcagcatcgc cgcacaaaat gtggctgtgc tgcttatctc ctctgatctg
     9661 gaagagatcg aactgatggc agatcgcgtg tatgtgatgc atcagggcga aattgcccac
     9721 tctgcactga ccggacgcga tattaatgtc gagaccatta tgcgcgttgc cttcggagat
     9781 agtcagcgtc aggaggcgtc atgctgaaat ttattcagaa caaccgtgaa atcacggcac
     9841 tgctggcggt ggtgctgctg tttgtattac ccggttttct cgaccgtcag tatttaagtg
     9901 tgcaaacgct gaccatggtt tatagcagcg cgcaaatcct gatcctgctg gcaatgggcg
     9961 cgacgctggt aatgcttacg cgcaatattg atgtttcagt gggttcgatt accggaatgt
    10021 gcgcggtgct gttggggatg ttactgaacg cggggtattc actacctgtt gcttgtgtcg
    10081 cgactttact gcttggtttg ctcgcgggat ttttcaacgg tgtcctggtc gcgtggctaa
    10141 agatccctgc cattgttgcc acccttggca cgttagggtt gtacagaggc atcatgttgc
    10201 tgtggactgg cggtaaatgg attgagggat tacccgccga actgaaacaa ctctccgccc
    10261 cgctgctgtt tggcgtttcc gcgattggtt ggttgacgat aattctggtg gcatttatgg
    10321 cctggctgct ggcaaagacg gcgtttggac gcagttttta tgtcacgggc gataatttac
    10381 agggcgctcg tcaactgggc gttcgtacgg aagccattcg cattgtggca ttttcgttga
    10441 acggctgcat ggcggcactg gcgggaattg tgtttgcttc gcagattggt tttatcccca
    10501 accagaccgg taccgtgctg gagatgaaag caattgcagc ctgcgtgctg ggcggcatta
    10561 gtttgctcgg cggttccggc gcgatcatcg gtgcggtact cggcgcatgg ttcctgacgc
    10621 agatcgatag tgtactggtg ctgttgcgca ttccggcatg gtggaatgat tttatcgcgg
    10681 gcatggttct gctggcggtg ctggtgtttg atggacgcct gcgttgtgcg ctggaacgta
    10741 atctgcggcg gcaaaaatat gcccgcttta tgatgccacc gccatccgtt aaacccgctt
    10801 cgtcaggtaa aaaacgggag gccgcgtaat gcgtattcgc tacggttggg aactggctct
    10861 tgccgcactg ctcgttattg agattgttgc atttggtgca attaacccgc ggatgttaga
    10921 tctcaatatg ttgctgttca gcaccagtga ctttatctgc attggcattg tcgccctacc
    10981 gctgacgatg gtgattgtca gtggcgggat cgatatttcg tttggttcga ccatcggcct
    11041 ctgcgccatt gcattgggcg tactgtttca aagtggtgtg ccgatgccgc tggcgattct
    11101 tctgacctta ctgctcggcg cattgtgcgg gctgatcaac gccggattaa ttatctatac
    11161 caaagttaac ccgctggtga ttacgcttgg cacgctgtat ctgtttgccg gaagcgccct
    11221 gctgctttcc gggatggccg gagcgacggg gtacgaaggt attggtggat tcccgatggc
    11281 gtttacagat ttcgctaacc tggatgtgct ggggctcccc gttccgctga ttatcttcct
    11341 gatatgtctc ctcgttttct ggctctggct gcataaaacc catgccggac gtaacgtgtt
    11401 tttaattggg caaagcccgc gtgtggcggt ttatagcgcg attccagtta accgcacctt
    11461 gtgtgcgctc tatgccatga cggggctggc gtctgcggtc gccgctgtgc tgctggtatc
    11521 gtattttggt tcagcacgtt ccgatctcgg tgcgtcgttt ctgatgcccg ccatcaccgc
    11581 cgtagtgctc ggcggtgcca atatttatgg tggttctggt tccattatcg gcaccgccat
    11641 agcggtttta ttagtgggat atttgcaaca aggtttgcaa atggcaggag tgccaaatca
    11701 ggtgtccagc gccctttccg gtgcgctact tatcgtcgtt gtcgtaggtc gttccgttag
    11761 cctgcatcgc cagcaaatta aagagtggct ggcgcgtcgg gccaataacc cattgccata
    11821 aaggatatct tcatgacact tcatcgcttt aagaaaatcg ccttacttag cgcgcttggc
    11881 attgccgtaa tctctatgaa tgtgcaggcg gcagagcgta ttgcatttat tcccaaactg
    11941 gttggcgtgg gattttttac cagcggtggc aacggcgcac aacaagcggg taaagagttg
    12001 ggcgttgatg tgacctacga cgggccgaca gaacccagtg tttctggtca ggtacagttg
    12061 attaataact tcgtcaatca ggggtataac gccattatcg tttctgcggt ttcgcctgat
    12121 ggcttgtgtc cggcactgaa acgcgccatg caacgtggtg tgagagtgct gacctgggac
    12181 tctgatacta aaccggagtg ccgctcttac tacattaatc agggaacgcc cgcccagttg
    12241 ggaggtatgt tggtggatat ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg
    12301 tttttctact caagccccac cgttacggac caaaaccagt gggtgaaaga agcgaaagcg
    12361 aaaatcgcca aagagcatcc aggctgggaa attgtcacta cgcagtttgg ctataacgat
    12421 gccactaaat cgttacaaac cgcagaagga atattaaaag cctatagcga tctcgacgcc
    12481 attatcgccc ccgatgccaa tgccctgccc gctgccgcac aagccgcaga aaacctgaaa
    12541 aatgacaaag ttgcgattgt cggattcagt acgccaaacg tgatgcgtcc atatgtggaa
    12601 cgcggcacgg tgaaagaatt tggcctgtgg gatgtggttc agcaaggcaa aatatcagtt
    12661 tatgtcgcgg atgcattatt gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc
    12721 cagggcgtag gtcaggtgga agtctcgcca aacagcgttc agggctatga ctacgaagct
    12781 gatggtaatg gcatcgtact gttaccggag cgcgtgatat tcaacaaaga gaatatcggc
    12841 aaatacgatt tctgatgtgc attacttaac cggagtacgt tatggcagat ttagatgata
    12901 ttaaagatgg taaagatttt cgtaccgatc aaccgcaaca aaatatccct tttaccctga
    12961 aaggttgcgg tgcactggat tggggaatgc agtcacgctt atcgcggata tttaatccga
    13021 aaacgggtaa aaccgtgatg ctggcctttg atcatggtta ttttcagggg ccgactaccg
    13081 gacttgaacg cattgatata aatatcgccc cgctgtttga acatgccgat gtattaatgt
    13141 gtacgcgcgg cattttgcgc agcgtagtcc ccccggcgac caataagccg gtggtactgc
    13201 gggcgtcagg tgcgaactct attctggcgg aattaagtaa tgaagccgtg gcgttatcga
    13261 tggatgatgc cgtgcgcctg aacagttgcg cggtggcggc gcaggtttat atcggcagcg
    13321 aatatgaaca tcagtcgatc aaaaatatta ttcagctggt cgatgccgga atgaaagcgg
    13381 gaataccgac catggccgtg actggtgtgg gcaaagatat ggtgcgcgat cagcgttatt
    13441 tctcgctcgc gactcgaatc gccgctgaaa tgggggcaca gattatcaaa acttattatg
    13501 tcgataaagg ttttgaacgg attgttgccg gatgtccggt acccattgtt attgctggcg
    13561 gtaaaaagtt accggagcgc gaggcgcttg aaatgtgctg gcaggctatc gatcagggcg
    13621 cttctggtgt ggatatgggg cgtaatattt tccagtctga ccatccggtg gcgatgatga
    13681 aagccgtaca ggcggtggta caccataacg aaacggctga tcgggcatat gaactctatc
    13741 tgagtgaaaa acagtaactg cggatttaag gagaagaatt atgcacgtca cactggttga
    13801 aattaacgtt catgaagata aggttgacga gtttatcgaa gtttttcgcc agaaccacct
    13861 gggctctgta caggaagaag gcaatttgcg cttcgatgtc ttacaggacc cggaagtgaa
    13921 ttcgcgcttt tatatctacg aagcctataa agatgaagac gcagtggcat tccataaaac
    13981 cacgccccac tacaaaacct gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa
    14041 aaaacgtctg ttcaatgggt tgatgccgtg aggcgaattt atcagtttta tctacaattg
    14101 gggtaacgcg ctgacgggag taaataaatg tctgactgga acccctcttt atatctgcac
    14161 tttgccgctg aacgatcgcg tccggcggtg gagctgcttg ccagagtgcc gctggagaat
    14221 atcaaatacg ttgccgatct tggctgtggc ccaggtaaca gcaccaccct gctgcatcaa
    14281 cgttggcctg cggccaggat aacaggcatc gactcgtctc cggcgatgat tgctgaagcg
    14341 cgcagtactt tgccagactg ccagttcgtg gaagcagaca tccgcaactg gcaaccggaa
    14401 caggcactcg atctgatttt tgctaatgcc tcactgcaat ggctgcccga ccactacgaa
    14461 ttgttccctc atctggtttc tttacttaat tcgcatggtg tgttggcagt acagatgcca
    14521 gataactggc tagagccaac ccatgtgctc atgcgcgaag tcgcctggga acaaaattat
    14581 ccggatcgcg ggcgtgagcc gctggctggc gttcatgctt actacgatat tctgagtgaa
    14641 gccggatgtg aggtcgatat ctggcgaacc acttactatc accagatgcc gtcgcaccag
    14701 tcgattatcg attgggtgac cgccactgga ttacgtccgt ggttacagga tctgaccgag
    14761 agcgaacagc agcattttct tacgcgctac catcagatgc tggaagagca atatcccctc
    14821 caggagaacg gacagatatt gctggcattt ccgcgtctgt ttattgttgc ccgccgtacg
    14881 gagtaattat cacgtcagct ggtaatggcg atcgggaaga attttcgctg gaatttctgc
    14941 ttcatcgttc atctgtaaca ggtcaatttc aatagcgttg cagatggcat ccagcggtaa
    15001 gtcattgttt tcagtaccga agggatcttc cagttcttcc gccagacaat ccagcgaaat
    15061 aaaagtgtag gaaatcagca cagagataaa aggcgtcatg taatgcaggt ccacgaccag
    15121 cgcaaacggc agcatgatac aaaacagata cacggtgcga tgcaaaatca gggtgtaggc
    15181 aaagggaatt ggcgtatagg caatgcgctc gcatcctgcc aggaccactg aaatatcatt
    15241 aagccgatcg ttgaggctaa taaacaggat atctgaaagt tgtccattgc gtcgctgaac
    15301 cgccaaccac tcccccatta ttaacaagat gcggttagct ggagagttag aagccagtac
    15361 acgctgaaga tcttcagtct tgagataatg agccagcact tccgcctgtg gctgtttgcg
    15421 taatgtcatg cgtaaacagt gggcgaaggc gatttgcagc cgggcaaact cccttacact
    15481 tgccgaatcc ggcaatgtcg tttttacctc gcgcagtaac gaccgcgagg caatcatcaa
    15541 ctgcccccaa agttttcgcg cttcaacgta acgagcgtaa ccggcattat tacgaaaacc
    15601 aagaaaaatg gcgatggcga caccgagaat gctgaacggt gcgagggtga atttaatgcc
    15661 caaatgcgtg taccagggca gcatgaaaat aacagcgata gaaaagagaa aattgagtag
    15721 taagcgcgag gatatcttgg ataatactga accgtgccag acaaaaatac ggcgcagcca
    15781 gtgttgttgt ggacgaacaa tcatggttat cttcaggcgt ggaaaagtcg ccctattaaa
    15841 cgtgattacg atcacattct caagacacta cttacaaatt acctacaagc ttacaacatt
    15901 aacaggtgct gcatgtggca cctgttaatg ataaaggcgc ataggatgtt gtaactaata
    15961 tagtgaaata agaaacccgg tcgaaaccgg gttcagagta ttagcacaac ggacgtacag
    16021 cttcgcgcat tccttttttg agaatcgcat ccaggtcatt agccacctgc tcgaccagac
    16081 caggcacttg cgtcaggtcc tgctcccagt ggtctttttc tgccaacaca atcgctacca
    16141 gttcctgggt accgataaca cggtcacgat gctggctcca gagttgctga taacgttcca
    16201 gccagtgtgc atcgtcctgt accggatatg tttctccatt acgctcaccg cgataaaacg
    16261 caatcaatgc cgctaatgcg aaagtcaggc gcgccggaag tgtgccgttt gccttctgcc
    16321 ctgccagcag ctgcggcagg atacgggtgc ggaacttggt cataccgtta agcgcgatag
    16381 acagcagctg atgcttgatg tacggattac ggaaacgtcc ggtgactgca ctggcgaaag
    16441 attccagttc atcacgaggc aaatccagta ccggaataat ctcttcgtag atggcttttt
    16501 caacgaatgc gcaaatttca gcatcgttca tcgcttcacc tacggtatcc agtcctgcct
    16561 ggaacgccac cggcaccagc ccggtgtgcg caccgttgag gattgccact ttgcgctctt
    16621 tatacggttt aatatcgtca acaatcagca cgttgagtgg atatttgtcc aggcgcagct
    16681 cagtcgctaa ggatttcggc ccctgaatca caaacaggta aaagtgttca gcggtgtcga
    16741 ggaaaccatc gtgataaccc agttcttctt ccagtttagc cacttcatcg cgcggataac
    16801 cggtaacgat acggtccacc agcgtagaac agaagctgtt agcctgatcc agccattgaa
    16861 taaatgcttc tggcagtgcc cactcttgcg catagcgcag aaccagttca cgcaacgcgt
    16921 cgccattgta gtcgatcaac tcacacggga tgatgatcca acctttatcc agcgcaccgt
    16981 tgaaatggct aaaacgttcg aacagcagac gggtcagttt tgccggataa cttaccgctg
    17041 gcgcgtcatc gaatttatcg cccgcatggt agctgatacc cgcttcggtg gtgttagaga
    17101 aaacaaagcg catttccggg ttgtgcgcca gtttcaggaa ttcatcgtat tcactgtaga
    17161 cgctgatttc acgattaaca gagcgaatca gacgcgcgtc gctgaccgct tcccccttct
    17221 cattcaggcc acggataatg gtggtgtaca gaccatcctg tgtgctcagt gacggtggga
    17281 atgaagtttc aatcggacga acaacgacca cgccagaatt cagatcggtg tgctcattca
    17341 ggagatcaat ttgccagtca acaaaggcgc gcaggaagtt accttcacca aactgaatga
    17401 tacgttctgg atactgtgca ccgggaaaat cgcgacgatt tagtgttttc acaatgggtt
    17461 cccttctgat tagtcataca acctgtttta attggtacga caggttagca aactttaata
    17521 cgccgaaccc ctgttttgat caactcctga tgattaatga gcagttttat gagaaaagtg
    17581 tggcgcggat catggtttaa tcgaggaact acgcctttta ttcgctcaat aaagtggtag
    17641 aacacattcc gttaaaatcg ctgtgaatga ataaagatgt cagacaactt cctcaccgta
    17701 acgcatagtg ctggtacggt tgcgcccatc tttcttcgag cgatacaaac atgtatcggc
    17761 ttcgaccatc aatttattaa agtcatcggt cagcgttcgg taggatgcgc aaccactacc
    17821 gacgccaata cttaccgtga gataaagcgt tttttgttgc caggtgaacg gttgcagttc
    17881 aacgccttta cggatttttt ccgccattag cagaccatcg acaggattca ccgatggcac
    17941 tgcaacagca aattcttcgc cgcccattcg cgccaccagc cccttatcac cgacaatctt
    18001 ctgaatatgc tgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc
    18061 gttaatactt ttgaaatagt cgatatcaag cagcatgaca gtcagatgtt gtgtctgttt
    18121 cagcgatgga cttttcagcg cctcataaag accggaccgt gagtaaacct gagtcagaaa
    18181 atcaaagtcg gctcgcagcg caacttgctt cattagcgaa ttgatcgctg ccacgctaaa
    18241 agaaaccata attgggcata tcgccatcgt ggcaataccg agacgtgcgg agaacatttg
    18301 cggaatggag aacggcgaac cgaccgagat atcaatcacc gaatttgcca ccagtacgat
    18361 ttccaccgca ccggtgacaa aggtcaacag acatgttacc tgcggcgtat agcgcactgc
    18421 acaccagatt aatgcaggca gcgggaacgc cagactcccc gctccgccaa tgactaccga
    18481 ggcaataacc gacacaataa gcgcgatagc gggcatcatc tgttctgctt taaagcgcgg
    18541 cagcactcca ggaattgcca acgtcagcat acaaggcacg atcaacacgc ccgttgagaa
    18601 ttgctcactg aaccagtccg caagcaaagg ccagaaatcc agactatcaa tactgaccga
    18661 accaatcgcc ccgacaatag cgcataataa tgcggcaatc agacagtaat taaatagccg
    18721 taaggcactt actggctcat acttattttt tcccagacgc ttatcccgcg cgaccagtaa
    18781 ggcgacggta acaataaaca tcatattgga gaaattgata gccagtgaaa ccagccccca
    18841 ttcggtggtt atggcatcat aaaccagcat cgccacataa ctgatcgcat agtaatgcag
    18901 tcgattaagc cagacatagc gggcaaacac ccccgccatt acgccattca gcggccagaa
    18961 aagagaaagt tcgtgaacca gccgtaactc tgcaccaata aaatagaaca gcgtggttag
    19021 cacaaaaata gcgatgctat tacgtagcag atgaccttcc tggaacaaac ggaaagttga
    19081 aatgggctgt acatgcatta attatctgtc catacaattg cgggtggcaa ttatctcaac
    19141 gcaataagta aattcttaga aaggatagca tgttcgctga caacggcggc aactaaagat
    19201 atcctgggaa tgctatttta tcgatttccg taattatcat ggctgattca tctgtataac
    19261 cagccagtgg tagattaccg tcacaatata acgttgttgc tcctcactta acgcttcccc
    19321 ctgaggaatg ttatgccact tagccagaca accgcgacaa caggtcgcgg tggcgtgttg
    19381 agcgataaaa accggatgcc cgcgcatggg ggtttgctta ccgtcgttag ccggtaatgc
    19441 cggagcaaga cgtttagcga caaaatccgc cgcatgttgt tcaatgactg gcgcgccttt
    19501 ctccagacaa tactgacgct ctttcacacc caaacgaaag cgagagcgaa attttgaacg
    19561 ggataaacgc gcgaagagtg gatcgagaga ctgcattaat aaactgaacg ccctaattgt
    19621 ttcgtcaatt gttcaaggac ggcaatgccc gcaagcgagt taccggcatc atccagttcc
    19681 ggactccaga ctgcgatggc catttcatgc ggaacaatcg ccacaatacc gccaccaacg
    19741 ccagattttg ccggtagtcc cacccgccag gcaaactccc ccgcgttctg gtacataccg
    19801 ctggtcgcca tcagcgcgtt aatttgccgc gcctgcattg gcgtcaccac aggttcatca
    19861 atatgaatag ctttcccctg attcgccaga aagacaaacg tccgggccag ctctatacag
    19921 ctcattttca gagcgcagta atgaaagtag ttttgcagaa cggttgtcac gtcatgatgg
    19981 aaattgccaa acgacttcat cagccaggcg atagccgcat ttcgcgcgga atgttcaaat
    20041 tcggaacgcg ctaccaccgt atcgtaggaa atatcagaca caccacttaa gccgcgcacg
    20101 acttccagca tacgttgccg aggtgcgctt aatcgccctt gcaacatatc gcagaccacc
    20161 agcgcaccgg cattaataaa cggattacgc ggaataccct gctccatttc cagttgcact
    20221 aaagaattga acggtgatcc agacggatct ttgccgacgc gttgccagat ttcctcttcg
    20281 gagtaatgac gcatggcgac aacgagactc agcactttgg aaatagactg aatggaaaaa
    20341 cgttcttgcg cgtctccggc ctgaaaaagc tgtccgtcaa cggtacagat agcaatcccc
    20401 attcgggaac cgtctaccgt agccagcgcc ggaatataat ccgcgacttt accctgacca
    20461 atgagcggcc gcacttgccg caagatgttt tctaaaattg cattatccat ggcgactgcc
    20521 actttctact cctggaccgc aggtctgaaa agacctgcga gtatatcaga gctgaatatg
    20581 tagcgtcaga tccggtcttt ccacaccgtc tggatattac agaattcgtg taagccaaaa
    20641 tgggaaagct cacgcccaaa gccactcttc ttcacgccac caaaagcgac tcgcgcgtcg
    20701 ctggcacagt aaccattgat aaacacccca ccgcattcca gacgtgccgc catctgtctg
    20761 gcccgggttt cgtcggtggt gaaaatggtc gctgaaaggc cgaactcgct atcattagcc
    20821 agttccagtg catgttctgc atctttcgca atggtgattg ccgcaacagg gccaaacatt
    20881 tcttcccgaa acgcggtcat ttctggggta acgttcgcca gaaccgttgg cggatagtag
    20941 ttacctgccc cagccatctt ttccccgccc agtaacaaac gcgcaccctg cgccagggtt
    21001 ttttccacct gatgatgcag ctcatcacgt aaatcaaaac gagccattgg tccgagagcg
    21061 ttctcttcgt cacggggatc gcccattttc aaggctgccg cagctgccac aaaacgttcg
    21121 gtaaatgccg aagcaattcc ctcttcgata ataaagcgtt ttgccgccgc acaaacctgt
    21181 ccggtattct gataacgtcc ggctaccgcc gctttaaccg ccagttccag atcggcatcg
    21241 ttaagcacaa taaatggatc cgaaccgccc agttccagta cgcatttttt cagtgccgct
    21301 ccagcctgtg cgccaatagc cgctcccgca cgaacacttc cggtcactgt gacagcagca
    21361 atgcgcgaat ctttaatcat ctgactgaca ccgttgttgt cagcattcag ccagccatat
    21421 acgccttgcg ggatacccgc atctttaaac acctgggcaa tgagctgtgc acagcccatc
    21481 acattcggcg catgtttaag taagtagccg ttacctgcaa gaatgatggg aaccgcgcca
    21541 cgcatcacct gccataacgg aaaattccac ggcataatcg ccagaattgt ccccaacggt
    21601 cgatactcaa taactgcctg ctgattttcc accagcgtag gttccgcctt cagcattgcc
    21661 ggaccatgtt ctgcatacca gtcacacaaa ttcgccgatt tcgccacttc agcgcgcgcc
    21721 tggttgattg gtttgcccat ttcacgggtg atcatttgcg ccatttcttc gctacggaca
    21781 cgcagagcct taccgatatc acgcagtttt tcagcacgat aatctatatt tgtctcgcgc
    21841 cagtcgcgaa agcctgctgc cgccagctga agtgcgtttt cgatatcatc agcgccagcc
    21901 cacggcagca cagaaagttg ttcaccggtg gcaggattta tcgaaattgc atgagttgcc
    21961 ggagtaatgg tcatcggggt atctccttta tgagttatgg taaaaagata cgcagattta
    22021 ctcttgcttt aaaatgaata atattaagcc acttattcac gaatcgagaa tattatggat
    22081 ctgacccaac tggagatgtt caacgcggtt gccgaagctg gcagcattac gcaggctgca
    22141 acaaaagtgc atcgcgtgcc gtcgaatctc actacccgtc tacgccagct ggaaacagaa
    22201 ctgggggttg atctgtttat tcgcgagaat cagcgtttac gtctctcgcc tgccgggcat
    22261 aactttttac gctatagcca acaaattctc gcgttagtgg atgaagcgcg gagcgttgtc
    22321 gctggcgatg agccgcaagg tttattttct cttggttcgc tggaaagcac cgcagcagtg
    22381 cgcattccgg ccatgctggc ggaatttaac cgtcgttatc ccaaaattca gttttcactt
    22441 tccaccggcc cttccggcac tatgctggaa ggggtgctgg agggaaaact gaatgcggcg
    22501 tttattgatg ggcccattaa ccatactgcc attgacggga taccggtgta ccgcgaggag
    22561 ctgatgattg tcacgccaca aggacatgcg ccagtaaccc gtgccagtca ggttaatggc
    22621 agtaacattt atgccttccg cgccaattgt tcgtatcgtc gccacttcga gagctggttt
    22681 catgctgacg gtgccgctcc gggaactatt cacgagatgg agtcgtatca cgggatgttg
    22741 gcctgtgtgg tcgcaggagc aggcattgcg cttattccac gctcaatgct ggaaagtatg
    22801 ccggggcatc accaggttga agcgtggccg ttagctgagc aatggcgttg gttaacaacc
    22861 tggctggtct ggcgtcgcgg tgcgaaaacg cgtccgcttg aggcatttat tcaactactg
    22921 gatgtgtctg actcggcaaa acagagttag taatgagcta tttttgatag ttcttgcggc
    22981 taatatgctc tatatagtga tgctccgatg acttatgact atatggggca aatatggtta
    23041 cgccagtaag catcagcaat ttcatctcac ttcctgatga ttttcccgtt cgtaatattg
    23101 cacctcaggt aaaagaagaa caggccccct tcctgatcgt cattaaaatg tgatgttaac
    23161 aactctctga tgcgtgacag aacaaccgtc agagagatgg aaatgcgatc cagatcacaa
    23221 atgcattgta ttcacatcat taaccgtttt aagatcattt catcactttt tcgcaactca
    23281 cccgataatc tgttatgaca acaaacactg tttcccgcaa agtggcgtgg ctacgggtcg
    23341 ttacgctggc agtcgccgcc ttcatcttca acaccaccga atttgtccca gttggcctgc
    23401 tctctgacat tgcgcagagt tttcacatgc aaaccgctca ggtcggcatt atgttgacca
    23461 tttacgcatg ggtagtagcg ctaatgtcat tgccttttat gttaatgacc agccaggttg
    23521 aacggcgcaa attactgatc tgcctgtttg tggtgtttat tgccagccac gtactgtcgt
    23581 ttttatcgtg gagttttacc gttctggtga tcagtcgcat tggtgtggct tttgcacatg
    23641 cgattttctg gtcgattacg gcgtctctgg cgatccgtat ggctccggcc gggaagcgag
    23701 cacaggcatt gagtttaatt gccaccggta ctgcactggc gatggtctta ggtttacctc
    23761 tcgggcgcat tgtggggcag tatttcggtt ggcgaatgac cttcttcgcg attggtatcg
    23821 gggagcttat cacccttttg tgcctgatta agttacttcc cttactgccc agtgagcatt
    23881 ccggttcgct gaaaagcctc ccgctattat tccgccgccc agcattgatg agcatttatt
    23941 tgttaactgt ggtggttgtc accgcccatt acacggcata cagctatatc gagccttttg
    24001 tgcaaaacat tgcgggattc agcgccaact ttgccacggc attactgtta ttactcggtg
    24061 gtgcgggcat tattggcagc gtgattttcg gtaaactggg taatcagtat gcgtctgcgt
    24121 tggtaagtac ggcgattgcg ctgttgctgg tgtgcctggc actgctgcta cctgcggcga
    24181 acagtgaaat acacctcggg gtgctgagta ttttctgggg gatagcgatg atgatcatcg
    24241 ggcttggtat gcaggttaaa gtgctggcgc tggcaccaga tgctaccgac gtcgcgatgg
    24301 cgctattctc cgggatattt aatattggaa tcggcgcggg tgcgttggta ggtaatcagg
    24361 taagtctgca ctggtcaatg tcgatgattg gttatgtggg cgcggtgcct gcctttgccg
    24421 cgttaatatg gtcaatcatc attttccgcc gctggccagt gacactcgaa gaacagacgc
    24481 aatagttgaa aggcccattt gggccttttt tagtggtacg ttttaatgat ttccaggatg
    24541 ccgttaataa taaactgcac ccccatacat accagcagga atcccatcag acgggagatc
    24601 gcttcaatgc cccccttacc caccagccgc ataattgcgc cagagctgcg tagacttccc
    24661 cacaaaataa ccgccaccag gaaaaagatc agcggcggcg caaccatcag tacccaatca
    24721 gcaaaggttg aactctgacg cactgtggac gccgagctaa taatcatcgc tatggttccc
    24781 ggaccggcgg tacttggcat tgccagtggt acaaaagcaa tattggcgct gggttcatct
    24841 tccagctctt ccgacttgct tttcgcttct ggtgaatcaa tcgctttctg ttgcggaaag
    24901 agcatccgaa aaccgataaa cgcgacgatt aagccgcctg caattcgcag accgggaatc
    24961 gaaatgccaa atgtatccat caccagttgc ccggcgtaat acgccaccat catgatggca
    25021 aagacgtaca ccgaggccat caacgactga cgattacgtt cggcactgtt catgttgcct
    25081 gccaggccaa gaaataacgc gacagttgtt aatgggttag ctaacggtag taacaccacc
    25141 agccccaggc caattgcttt aaataaatct aacattggtg gttgttatcc tgtgtatctg
    25201 gtttatcagc gaaaagtata aggggtaaac aacgataaag tgtcactctt tagctaacct
    25261 tgcatcgcat tgaccaaaac ttgaaccgat ttagcaaaac gtggcatcgg tcaattcatt
    25321 catttgactt atacttgcct gggcaatatt atcccttgca actaattact tgccagggca
    25381 actaatgtga aaagtaccag cgatctgttc aatgaaatta ttccattggg tcgcttaatc
    25441 catatggtta atcagaagaa agatcgcctg cttaacgagt atctgtctcc gctggatatt
    25501 accgcggcac agtttaaggt gctctgctct atccgctgcg cggcgtgtat tactccggtt
    25561 gaactgaaaa aggtattgtc ggtcgacctg ggggcgctga cccgtatgct ggatcgcctg
    25621 gtctgtaaag gctgggtgga aaggttgccg aacccgaatg acaagcgcgg cgtactggta
    25681 aaacttacca ccagcggcgc ggcaatatgt gaacaatgcc atcaattagt tggccaggac
    25741 ctgcatcaag aattaacaaa aaacctgacg gcggacgaag tggcaacact tgagcatttg
    25801 cttaagaaag tcctgccgta aacaaaaaaa gaggtatgac gatgtccaga cgcaatactg
    25861 acgctattac cattcatagc attttggact ggatcgagga caacctggaa tcgccactgt
    25921 cactggagaa agtgtcagag cgttcgggtt actccaaatg gcacctgcaa cggatgttta
    25981 aaaaagaaac cggtcattca ttaggccaat acatccgcag ccgtaagatg acggaaatcg
    26041 cgcaaaagct gaaggaaagt aacgagccga tactctatct ggcagaacga tatggcttcg
    26101 agtcgcaaca aactctgacc cgaaccttca aaaattactt tgatgttccg ccacataaat
    26161 accggatgac caatatgcag ggtgaatcgc gctttttaca tccattaaat cattacaaca
    26221 actagttgat aacgtgacaa cgtcactgag gcaatcatga aaccactttt atccgcaata
    26281 gcaactgcgc ttattctctt ttctgcgcag ggcgttgcgg aacaaaccac gcagccggtt
    26341 gttacttcct gtggcaatgt cgtggttgtt cccacatcgc aggaacaacc accgtttgat
    26401 ttaaatcaca tgggtactgg cagtgataag tcggatgcgc tcggcgtgcc ctattataat
    26461 caacacgcta tgtagtttgt tctggccccg acatatcggg gcttattaac ttcccacttt
    26521 taccgcttta cgccaccgca agccaaatac attgatatac agcccggtca taatgagcac
    26581 cgcaccgaaa aattgtagac ccgttaagcg ttcatccaat aatagtgccg cacttgccag
    26641 tcctactacg ggcaccagta acgataacgg tgcaacccgc caggtttcat agcgtcccag
    26701 taacgtcccc cagatcccat aaccaacaat tgtcgccaca aacgccagat acatcagaga
    26761 caagatggtg gtcatatcga tagtaaccag actgtgaatc atggttgcgg aaccatcgag
    26821 aatcagcgag gcaacaaaga agggaatgat tgggattaat gcgctccaga ttaccagcga
    26881 catcaccgcc gggcgcgttg agtgcgacat gatcttttta ttgaagatgt tgccacacgc
    26941 ccaactaaat gctgccgcca gggtcaacat aaagccgagc atcgccacat gctgaccgtt
    27001 cagactatct tcgattaaca ccagtacgcc aaaaatcgct aaggcgatcc ccgccaattg
    27061 tttgccatgc agtcgctccc cgaaagtaaa cgcgccaagc acgatagtaa aaaacgcctg
    27121 tgcctgtaac accagcgaag ccagtccagc aggcatacca aagttaatgg cgcaaaacag
    27181 aaaggcaaac tgcgcaaaac tgatggttaa tccatacccc agcagcaaat tcagtggtac
    27241 tttcggtcga gcgacaaaaa agatggccgg aaaagcgacc agcataaagc gcaaaccggc
    27301 cagcatcagc ggtggcatgt tatgaagccc cactttgatg accacaaaat tcagccccca
    27361 tacgaccact accagtagcg ccaacacccc atcttttcgc gacattatac cgcctctgaa
    27421 tttcatcttt tgtaagcaat caacttagct gaatttactt ttctttaaca gtcgattcgt
    27481 tagtcaccgt ttacgacggc attaatgcgc aaataagtcg ctatacttcg gatttttgcc
    27541 atgctatttc tttacatctc taaaacaaaa cataacgaaa cgcactgccg gacagacaaa
    27601 tgaacttatc cctacgacgc tctaccagcg cccttcttgc ctcgtcgttg ttattaacca
    27661 ttggacgcgg cgctacgctg ccgtttatga ccatttactt gagtcgccag tacaacctga
    27721 gtgtcgatct aatcggttat gcgatgacaa ttgcgctcac tattggcgtc gtttttagcc
    27781 tcggttttgg tatcctggcg gataagttcg acaagaaacg ctatatgtta ctggcaatta
    27841 ccgccttcgc cagcggtttt attgccattc ctttagtgaa taacgtgacg ctggtggtgc
    27901 tcttttttgc cctcattaac tgcgcctatt ccgtttttgc taccgtgttg aaagcctggt
    27961 ttgccgacaa tctttcgtcc accagcaaaa cgaaaatctt ctcaatcaac tacaccatgc
    28021 tcaacattgg ctggaccatc ggtccgccgc tcggcacgtt gttggtgatg cagagcatca
    28081 atctgccctt ttggctggca gctatctgtt ccgcgtttcc catgcttttc attcaaattt
    28141 gggtaaagcg caacgagaaa atcatcgcca cggaaacagg cagtgcctgg tcgccgaaag
    28201 ttttattaca agataaagca ctgttgtggt ttacctgctc tggttttctg gcttcctttg
    28261 ttagcggcgc atttgcctcg tgcatttcac aatatgtgat ggtgattgct gatggtgatt
    28321 ttgccgaaaa ggtggttgcg gttgttcttc cggttaatgc tgccatggta gttacgttgc
    28381 aatattccgt gggccgccga cttaacccgg ctaacatccg cgcgctgatg acagcaggca
    28441 ccctctgttt cgtcattggg ctggtcggtt ttattttttc cggcaacagc ctgctattgt
    28501 ggggtatgtc agctgcggta tttactgtcg gtgaaatcat ttatgcgccg ggcgagtata
    28561 tgttgattga ccatattgcg ccgccaggaa tgaaagccag ctatttttcc gctcagtctt
    28621 taggctggct tggtgcggcg attaacccat tagtgagtgg catagtgctt accagtctgc
    28681 cgcctttctc gctgtttatc atcttagcgt tggtgatcat tgctgcgtgg gtgctgatgt
    28741 taaaaggaat tcgtgcaaga ccgtgggggc agcccgcgct ttgttgattt aagtcgaaca
    28801 caataaaaat ttaattcagc cttcgtttag gttacctctg ctaatatctt tctcattgag
    28861 atgaaaatta aggtaagcga ggaaacacac cacaccataa acggaggcaa ataatgctgg
    28921 gtaatatgaa tgtttttatg gccgtactgg gaataatttt attttctggt tttctggccg
    28981 cgtatttcag ccacaaatgg gatgactaat gaacggagat aatccctcac ctaaccggcc
    29041 ccttgttaca actgtgtaca aggggcctga tttttatgac ggcgaaaaaa aaccgccagt
    29101 aaaccggcgg tgaatgcttg catggataga tttgtgtttt gcttttacgc taacaggcat
    29161 tttcctgcac tgataacgga tcgttgacac agtagcatca gttttctcaa tgaatgttaa
    29221 acggagctta aactcggtta atcacgtttt gttcgtcaat aaacatacag cgatttcttc
    29281 cggtttgctt accctcatac attgcccggt ccgctcttcc aatgaccaca tccagaggct
    29341 cttcaggaaa tgcgcgactc acacctgctg tcacggtaat gttgatatgc ccttcagaat
    29401 gtgtgatggc atggttatcg actaactggc aaattctgac acctacacga catgcttctt
    29461 catcattagc cgctttgaca ataatgataa attcttcgcc accgtagcga taaaccgttt
    29521 cgtaatcgcg cgtccaactg gctaagtaag ttgccagggt gcgtaatact acatcgccga
    29581 ttaaatgccc gtaggtatca ttaaccaatt taaatcggtc aatatccaac aacattaaat
    29641 aaagattcag aggctcagcg ttgcgtaact gatgatcaaa ggattcatca agaacccgac
    29701 gacccggcaa tcccgtcaaa acatccatat tgctacggat cgtcagcaaa taaattttgt
    29761 aatcggttaa tgccgcagta aaagaaagca acccttcctg aaaggcgtcg aaatgcgcgt
    29821 cctgccagtg attttcaaca atagccagca ttaattcccg accacagtta tgcatatgtt
    29881 gatgggcaga atccattagc cgaacgtaag gtaattcatc gttatcgagt ggtcccagat
    29941 gatcaatcca ccgaccaaac tggcacagtt cataagaatg gttatccgtt atttctggct
    30001 tactggcatc tctcgcgacc acgctgtgaa acatacttac cagccactgg tagtgggcat
    30061 cgatagcctt attgagattt aacaagatgg catcaatttc cgttgtcttc ttgatcattg
    30121 ccactccttt ttcacagttc cttgtgcgcg ctattctaac gagagaaaag caaaattacg
    30181 tcaatatttt catagaaatc cgaagttatg agtcatctct gagataacat tgtgatttaa
    30241 aacaaaatca gcggataaaa aagtgtttaa ttctgtaaat tacctctgca ttatcgtaaa
    30301 taaaaggatg acaaatagca taacccaata ccctaatggc ccagtagttt aggccatcag
    30361 gctaatttat ttttatttct gcaaatgagt gacccgaacg acggccggcg cgcttttctt
    30421 atccagactg ccgctaatgt tgatcatctg gtccggctga acttctcgtc catcaaagac
    30481 tgccgcagga ataacaacat taatttcacc actcttatcg cgaaaaacgt aacggtcctc
    30541 tcctttgtga gaaatcaaat taccgcgtag tgaaaccgaa gcgccatcgt gcatgttttt
    30601 tgcgaaatca acggtcattt tttttgcatc atcggttccg cgatagccat cttctattgc
    30661 atgaggcggc ggtggcgctg catcctgttt taatccgccc tggtcatctg ccaacgcata
    30721 aggcatgaca agaaaacttg ctaatacaat ggcctgaaat ttcatactga ctccttaatt
    30781 gcgtttggtt tgacttatta agtctggttg ctatttttat aattgacaaa taagaatact
    30841 gccaattgtt ataaggcatt taaaatcagg caactagctg tca
//
LOCUS       DAZUCC010000035        30434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZUCC010000035 DAZUCC010000000
VERSION     DAZUCC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.035"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(47..1297)
                     /gene="icd"
                     /locus_tag="ACHY09_001432"
     CDS             complement(47..1297)
                     /gene="icd"
                     /locus_tag="ACHY09_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFS3884422.1"
                     /translation="MESKVVVPAQGKKITLQNSKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1469..2122
                     /gene="rluE"
                     /locus_tag="ACHY09_001433"
     CDS             1469..2122
                     /gene="rluE"
                     /locus_tag="ACHY09_001433"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFS3884423.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTEDALEALRNGVTLNDGPTLPAGAEMVEEPEWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            2132..2593
                     /gene="nudJ"
                     /locus_tag="ACHY09_001434"
     CDS             2132..2593
                     /gene="nudJ"
                     /locus_tag="ACHY09_001434"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFS3884424.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2647..3753
                     /gene="mnmA"
                     /locus_tag="ACHY09_001435"
                     /gene_synonym="trmU"
     CDS             2647..3753
                     /gene="mnmA"
                     /locus_tag="ACHY09_001435"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS3884425.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVCRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYDGEVCLGGGIIEQRLPLPV"
     gene            3789..4430
                     /gene="hflD"
                     /locus_tag="ACHY09_001436"
     CDS             3789..4430
                     /gene="hflD"
                     /locus_tag="ACHY09_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFS3884426.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4434..5804
                     /gene="purB"
                     /locus_tag="ACHY09_001437"
     CDS             4434..5804
                     /gene="purB"
                     /locus_tag="ACHY09_001437"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS3884427.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5973..6644
                     /gene="phoP"
                     /locus_tag="ACHY09_001438"
     CDS             5973..6644
                     /gene="phoP"
                     /locus_tag="ACHY09_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFS3884428.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6644..8104
                     /gene="phoQ"
                     /locus_tag="ACHY09_001439"
     CDS             6644..8104
                     /gene="phoQ"
                     /locus_tag="ACHY09_001439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFS3884429.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8180..9301
                     /gene="roxA"
                     /locus_tag="ACHY09_001440"
                     /gene_synonym="ycfD"
     CDS             8180..9301
                     /gene="roxA"
                     /locus_tag="ACHY09_001440"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFS3884430.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETSWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9447..10676)
                     /gene="pepT"
                     /locus_tag="ACHY09_001441"
     CDS             complement(9447..10676)
                     /gene="pepT"
                     /locus_tag="ACHY09_001441"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS3884431.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10926..12062
                     /gene="potA"
                     /locus_tag="ACHY09_001442"
     CDS             10926..12062
                     /gene="potA"
                     /locus_tag="ACHY09_001442"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFS3884432.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPADEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFTVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12046..12903
                     /gene="potB"
                     /locus_tag="ACHY09_001443"
     CDS             12046..12903
                     /gene="potB"
                     /locus_tag="ACHY09_001443"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFS3884433.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12900..13694
                     /gene="potC"
                     /locus_tag="ACHY09_001444"
     CDS             12900..13694
                     /gene="potC"
                     /locus_tag="ACHY09_001444"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFS3884434.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13691..14737
                     /gene="potD"
                     /locus_tag="ACHY09_001445"
     CDS             13691..14737
                     /gene="potD"
                     /locus_tag="ACHY09_001445"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFS3884435.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14795..15256
                     /locus_tag="ACHY09_001446"
     CDS             14795..15256
                     /locus_tag="ACHY09_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFS3884436.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPYSKKTRRKRKSRP"
     gene            15253..16038
                     /locus_tag="ACHY09_001447"
     CDS             15253..16038
                     /locus_tag="ACHY09_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS3884437.1"
                     /translation="MKKIIILLSLLILLPLTVTSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHAASSMSNVKGSVLLLFTTRE
                     PRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDLPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAVA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(16164..16985)
                     /gene="cobB"
                     /locus_tag="ACHY09_001448"
     CDS             complement(16164..16985)
                     /gene="cobB"
                     /locus_tag="ACHY09_001448"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFS3884438.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17001..17912)
                     /gene="nagK"
                     /locus_tag="ACHY09_001449"
     CDS             complement(17001..17912)
                     /gene="nagK"
                     /locus_tag="ACHY09_001449"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFS3884439.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17941..19185)
                     /gene="lolE"
                     /locus_tag="ACHY09_001450"
     CDS             complement(17941..19185)
                     /gene="lolE"
                     /locus_tag="ACHY09_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFS3884440.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIADILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19185..19886)
                     /gene="lolD"
                     /locus_tag="ACHY09_001451"
     CDS             complement(19185..19886)
                     /gene="lolD"
                     /locus_tag="ACHY09_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFS3884441.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19879..21078)
                     /gene="lolC"
                     /locus_tag="ACHY09_001452"
     CDS             complement(19879..21078)
                     /gene="lolC"
                     /locus_tag="ACHY09_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFS3884442.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21340..22413
                     /locus_tag="ACHY09_001453"
     CDS             21340..22413
                     /locus_tag="ACHY09_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS3884443.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMTSLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22541..25987
                     /gene="mfd"
                     /locus_tag="ACHY09_001454"
     CDS             22541..25987
                     /gene="mfd"
                     /locus_tag="ACHY09_001454"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS3884444.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26131..27093
                     /gene="ldtC"
                     /locus_tag="ACHY09_001455"
     CDS             26131..27093
                     /gene="ldtC"
                     /locus_tag="ACHY09_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFS3884445.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27176..27433)
                     /gene="bhsA"
                     /locus_tag="ACHY09_001456"
                     /gene_synonym="ycfR"
     CDS             complement(27176..27433)
                     /gene="bhsA"
                     /locus_tag="ACHY09_001456"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFS3884446.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            27674..28306
                     /gene="comR"
                     /locus_tag="ACHY09_001457"
     CDS             27674..28306
                     /gene="comR"
                     /locus_tag="ACHY09_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFS3884447.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(28368..28907)
                     /locus_tag="ACHY09_001458"
     CDS             complement(28368..28907)
                     /locus_tag="ACHY09_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFS3884448.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(29134..>30434)
                     /gene="ndh"
                     /locus_tag="ACHY09_001459"
     CDS             complement(29134..>30434)
                     /gene="ndh"
                     /locus_tag="ACHY09_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFS3884449.1"
                     /translation="TPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHL
                     WKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKG
                     ELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYS
                     ANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERI
                     LPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKA
                     PDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQ
                     MATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIAR
                     FVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT         7380 a         7913 c         7717 g         7424 t
ORIGIN      
        1 aacgggcgtg ttatacgccc gtttcaatat ttaacacatg tagagattac atgttcttga
       61 tgatcgcatc accaaactcg ctgcatttca gcagtttagc gccttccatc agacgttcga
      121 agtcataggt tacagtcttg gcgttgattg cgccttccat acctttaaca atcaggtctg
      181 cggcttcagt ccagcccata tggcgtaaca tcatctcagc ggagagaata atagagccag
      241 ggttaacttt gtcctgaccg gcatatttcg gcgcagtacc gtgggtggct tcaaacaggg
      301 cgcattcgtc accgatgttt gcgcctgggg cgataccgat accgccgacc tgtgcagcca
      361 gagcgtcaga aatgtagtca ccgttcaggt tcatacaggc gataacatca tattcagccg
      421 gacgcagcag gatctgttgc aggaatgcat cagcaatcac atctttaatg acgatctctt
      481 tgccggtatt cgggttttta actttcagcc acgggccgcc gtcgatcagt tcaccgccaa
      541 actcttcacg cgccagctgg tagccccaat ctttaaacgc gccttcggtg aacttcatga
      601 tgttgccttt gtgcaccagg gtcacagagt cacgatcgtt cgcaattgcg tattcgatcg
      661 ctgcacgcac cagacgtttg gtgccttctt cagaacacgg cttaataccg ataccgcaat
      721 gttccgggaa acgaattttc ttcacgccca tctcttcacg caggaattta atcactttct
      781 cggcgtcggc agagtctgct ttccattcga tacccgcata aatgtcttcc gagttttcac
      841 ggaagataac catatcggtc agttcagggt gtttaaccgg gcttggggtg ccctggtagt
      901 aacgtaccgg acgcaggcag atgtagagat ccagttcctg gcgcagggca acgttcagag
      961 agcgaatacc gccaccaacc ggagtggtca gcgggccttt aatggcaacg cgatattcac
     1021 gaatcagatc aagggtttca gcaggcagcc agacatcctg accataaacc tgtgtggatt
     1081 tttcaccggt gtaaatttcc atccaggaga ttttacgctc gcctttatag gctttctcga
     1141 ctgcagcgtc gaccactttc agcatggctg gggttacatc tacaccgatt ccatcacctt
     1201 caatgtaagg gataatcgga ttttcaggaa cgttgagttt gctgttttgc agggtgatct
     1261 tcttgccttg tgccggaaca actactttac tttccattca cctctccttc gagcgctact
     1321 ggtttgctcg tctgccacca cgttgcatat gcgtttgcgt cctgcgatac ggatgcttta
     1381 gagcaatttt ttgttaatga tttgtaattg gcttgtcaat actaccctat tgtttgccgt
     1441 catgaaagat gcgcgttatt aggctataat gcggcaattc ataatctctg aaaataccat
     1501 gcaaaaaact tcttttagaa atcaccaggt taagcgattc agctcgcaac gttctaccag
     1561 gcgtaaacct gaaaaccagc ccacgcgtgt gatcctgttc aataaaccct acgatgttct
     1621 tccgcagttc accgatgaag ccggacgcaa aacattaaaa gaattcatcc cggttcaagg
     1681 cgtttatgca gcaggtcgcc ttgaccgcga tagcgaaggg ttactggtgc tgaccaataa
     1741 cggcgcgttg caggcgcgtt taacccagcc gggtaaacgt accgggaaaa tctattatgt
     1801 acaggtagaa ggtattccca cggaagacgc ccttgaagcg ttgcgcaatg gcgtaacctt
     1861 aaatgatggc cctaccctgc ccgcaggtgc agagatggtt gaggagccgg aatggttatg
     1921 gccccgtaat ccgccaattc gtgaacgcaa aagtattccc accagctggc tgaagatcac
     1981 cttatatgaa ggacgaaatc gccaggtgcg ccgcatgacc gcccatgttg gtttccccac
     2041 gctgcgactg attcgctatg cgatgggtga ttactctttg gataatcttg ccaatggcga
     2101 atggcgagaa gcgacagatt aaggacgcaa aatgtttaaa ccgcacgtta ccgttgcctg
     2161 cgtggtgcac gcagaaggca aatttttagt cgttgaagag acgattaatg gtaaagcgtt
     2221 atggaaccaa cctgccgggc atctggaagc ggatgagacg ctagtggaag ctgctgcgcg
     2281 tgagctgtgg gaagaaaccg gcatcagcgc gcagccgcaa cactttattc gtatgcatca
     2341 gtggattgcg ccagataaaa cgccgttttt gcgtttcctg tttgctattg agcttgagca
     2401 aatatgcccg acccagcctc atgacagcga tatcgactgc tgccgttggg tcagcgccga
     2461 agaaatttta caggcgtcaa atctgcgttc gccgctggtg gcggaaagta ttcgttgtta
     2521 tcaaagcggg caacgctatc cgctggagat gattggcgat tttaactggc cttttacaaa
     2581 gggtgtcatc taaaaggctg cgtgatagaa tacgccgcct tgaagttcaa tgtcgtgagt
     2641 gatccaatgt ctgaaaccgc aaaaaaagta atcgtcggca tgtccggcgg tgtcgattcc
     2701 tccgtttctg cctggctgtt gcaacaacag ggatatcagg tcgaaggcct gtttatgaag
     2761 aactgggaag aagacgacgg tgaggaatat tgcacagcgg cagcggatct ggctgatgcc
     2821 caggctgtct gcgacaagct cggcattgaa ctgcacaccg ttaactttgc tgccgagtac
     2881 tgggacaacg tcttcgaact gttccttgcc gaatataaag ccggtcgcac gccgaatccg
     2941 gatattctgt gcaacaaaga gatcaaattt aaagccttcc tcgaatttgc cgccgaagat
     3001 ttaggtgccg attatatcgc taccggtcat tacgtatgtc gggcagatgt cgatggcaag
     3061 agccgcctgc tgcgtggtct ggacagcaat aaagaccaga gctacttcct ttatacgctc
     3121 agccatgagc agattgcgca aagcctgttc ccggtcggcg aactggaaaa accgcaggtg
     3181 cgtaagattg ctgaagatct tggtctggtc accgcgaaga aaaaagactc taccggcatt
     3241 tgcttcattg gcgaacgtaa attccgcgag ttcctgggcc gttatctccc ggcgcaaccg
     3301 ggcaaaatca ttaccgtcga tggcgatgaa attggtgaac accaggggct gatgtatcac
     3361 accctcggtc agcgtaaagg tctgggtatt ggtggcacca aagaaggcac cgaagaaccg
     3421 tggtatgtgg tcgataaaga cgtcgaaaac aacattctga ttgttgcgca gggccatgaa
     3481 cacccgcgtc tgatgtctgt cggtctgatt gcccagcagt tgcactgggt cgatcgcgaa
     3541 ccattcaccg gcactatgcg ttgcacggta aaaacccgct atcgccagac cgacattccg
     3601 tgcaccgtca aggcgctgga cgatgatcgc attgaagtga ttttcgatga gccggttgcc
     3661 gccgtgacgc cgggccagtc tgccgtattc tatgacggtg aagtgtgcct cggtggcggt
     3721 attattgagc agcgtctgcc gctgccggtc tgattatttt ctttacttaa aacaaggaag
     3781 cagtgaacgt ggcaaagaat tactatgaca tcaccctcgc tctggccggt atttgtcagt
     3841 cggcacgcct ggtgcaacaa ctcgctcacc aggggcattg tgatgccgat gcgctacacg
     3901 tctcactcaa cagtattatt gatatgaacc ccagctcgac gctggcggtt tttggcggta
     3961 gcgaagccaa cctgcgcgtc gggctggaaa ccttgctcgg cgtgctcaat gccagcagtc
     4021 gccagggctt aaacgctgag ttaacccgct acacactcag cctgatggtg cttgagcgca
     4081 aactctcctc agcgaaaggt gcgctcgaca ctctgggcaa ccggatcaac ggcctgcaac
     4141 gccagctcga acacttcgat ttacagtccg aaacgctgat gagcgcgatg gctgctatct
     4201 atgttgatgt gattagcccg cttggcccgc gcattcaggt caccggttcc cctgctgtac
     4261 tgcaaagccc acaagtgcag gcgaaagttc gcgctaccct gctggcaggc attcgcgccg
     4321 ccgtgctctg gcaccaggtc ggcggcggac gtctgcaact gatgttttct cgtaatcgcc
     4381 tgaccactca ggcaaaacaa attcttgctc atttaacccc ggagttgtga tctatggaat
     4441 tatcctcact gaccgccgtt tcccctgtcg atggacgcta cggcgataaa gtcagcgcgc
     4501 tgcgcgggat tttcagcgaa tatggtttgc tgaaattccg tgtacaagtt gaagtacgtt
     4561 ggctgcaaaa actggccgcg cacgcagcga tcaaggaagt tcctgctttt gctgccgacg
     4621 caatcggtta ccttgatgca attgtcgcca gtttcagcga agaagatgca gcacgcatca
     4681 aaaccatcga gcgtaccact aaccacgacg ttaaagcggt tgagtatttc ctgaaagaaa
     4741 aagtggcgga gatcccggaa ctgcatgcgg tttctgaatt catccacttt gcctgtactt
     4801 cggaagatat caataacctc tcccacgcgt tgatgctgaa aaccgcgcgt gatgaagtga
     4861 tcctgccgta ctggcgtcaa ctgattgatg gcattaaaga tctcgccgtt cagtatcgcg
     4921 atatcccgct gctgtcccgt acccacggtc agccagccac gccgtcaacc atcggtaaag
     4981 agatggctaa cgtcgcctac cgtatggagc gccagtaccg ccagcttaat caggtggaga
     5041 tcctcggtaa aatcaacggc gcagtcggta actataacgc ccacatcgcc gcttacccgg
     5101 aagttgactg gcatcagttc agcgaagagt tcgtcacctc gctgggtatt cagtggaacc
     5161 cgtacactac ccagatcgaa ccgcacgact acattgccga gctgtttgat tgcgttgcgc
     5221 gcttcaacac cattctgatc gactttgacc gtgacgtctg gggttatatc gcccttaacc
     5281 acttcaaaca gaaaaccatt gctggtgaga ttggttcttc caccatgccg cataaagtta
     5341 acccgatcga cttcgaaaac tccgaaggga atctgggcct ttccaacgcg gtattgcagc
     5401 atctggcaag caaactgccg gtttcccgct ggcagcgtga cctgaccgac tccaccgtgc
     5461 tgcgtaacct cggcgtgggt atcggttatg cgctgattgc gtatcaatcc accctgaaag
     5521 gggtgagcaa actggaagtg aaccgtgacc atctgctgga tgaactggat cacaactggg
     5581 aagtgctggc tgagccaatc cagacagtta tgcgtcgcta tggcatcgaa aaaccgtacg
     5641 agaagctgaa agagctgact cgcggtaagc gcgttgacgc cgaaggcatg aagcagttta
     5701 tcgacggtct ggcgttgcca gaagaagaga aagcccgcct gaaagcgatg acgccggcta
     5761 actatattgg tcgcgccatc accatggttg atgagctgaa ataagcctca aatcagtgcc
     5821 ggatggcgat gctgtccggc ctgcttatta agattatcca ctttttgttt tttcacttta
     5881 cctccccttc ccgctggttt atttaatgtt tacccccata accacataat cgcgttacac
     5941 tattttaata attaagacag ggagaaataa aaatgcgcgt actggttgtt gaagacaatg
     6001 cgttgttacg tcaccatctt aaagttcaga ttcaggatgc tggtcatcag gtcgatgacg
     6061 cagaagatgc caaagaagcc gattattatc tcaatgaaca tttaccggat attgcgattg
     6121 tcgatctcgg tttgcctgac gaggacggtc tgtcactgat tcgccgctgg cgtagcaatg
     6181 atgtttcact gccgattctg gtattaaccg cccgtgaaag ctggcaggac aaagtcgaag
     6241 tattaagtgc tggcgctgat gattacgtga ctaaaccgtt tcatattgaa gaggtgatgg
     6301 cgcgaatgca ggcattaatg cggcgtaata gcggtctggc ttcacaggtc atttcgctac
     6361 caccgtttca ggttgatctc tctcgccgtg aattatctat taatgacgaa gtgatcaaac
     6421 tgaccgcgtt cgaatacacc attatggaaa cgttgatacg caataatggc aaagtggtga
     6481 gcaaagattc gttaatgctc caactctatc cggatgcgga gctgcgggaa agccatacca
     6541 ttgatgtact gatgggacgt ctgcgcaaaa aaattcaggc acaatatccg caagaagtga
     6601 ttaccaccgt tcgcggccag ggctatctgt tcgaattgcg ctgatgaaaa aattactgcg
     6661 tctttttttc ccgctctcgc tgcgggtacg ttttctgttg gcaacggcag cggtagtact
     6721 ggtgctttcg cttgcctacg gaatggtcgc gctgatcggt tatagcgtca gtttcgataa
     6781 aaccacgttt cggctgttac gtggcgagag caatctgttc tatacccttg cgaagtggga
     6841 aaacaataag ttgcatgtcg agttacccga aaatatcgac aagcaaagcc ccacgatgac
     6901 actaatttat gatgagaacg ggcagctttt atgggcgcaa cgtgacgtgc cctggctgat
     6961 gaagatgatc cagcctgact ggctgaaatc gaatggtttt catgaaattg aagcggatgt
     7021 taacgatacc agcctcttgc tgagtggaga tcattcgata cagcaacagt tgcaggaagt
     7081 gcgggaagat gatgacgacg cggagatgac ccactcggtg gcggtaaacg tctacccggc
     7141 aacatcgcgg atgccaaagt tgaccattgt ggtggtggat accattccgg tggagctaaa
     7201 aagttcctat atggtctgga gctggtttat ctatgtgctc tcagccaatc tgctgttagt
     7261 gatcccgctg ctgtgggtcg ccgcctggtg gagtttacgc cccatcgaag ccctggcaaa
     7321 agaagtccgc gaactggaag aacataaccg cgaattgctc aatccagcca caacgcgaga
     7381 actgaccagt ctggtacgaa acctgaaccg attgttaaaa agtgaacgcg aacgttacga
     7441 caaatatcgt acgacgctca ccgacctgac ccatagtctg aaaacgccac tggcggtgct
     7501 gcaaagtacg ctgcgttctc tgcgtagtga aaagatgagc gtcagtgatg ctgaaccggt
     7561 aatgctggag caaatcagcc gcatttcaca gcaaattggc tactacctgc atcgtgccag
     7621 tatgcgcggc gggacattgc tcagccgcga gctgcatccg gtcgctccac tgctggacaa
     7681 tctcacctcg gcgctgaaca aagtgtatca acgcaaaggg gtcaatatct ctctcgatat
     7741 ttcgccagag atcagctttg tcggtgagca gaacgatttt gtcgaggtga tgggcaatgt
     7801 gctggataat gcctgtaaat attgcctcga gtttgtcgaa atttctgcaa ggcaaaccga
     7861 cgagcatctc tatattgtgg tcgaggatga tggccccggt attccattaa gcaagcgaga
     7921 ggtcattttc gaccgtggtc aacgggttga tactttacgc cctgggcaag gtgtggggct
     7981 ggcggtagcc cgcgaaatca ccgagcaata tgagggtaaa atcgtcgccg gagagagcat
     8041 gctgggcggt gcgcggatgg aggtgatttt tggtcgccag cattctgcgc cgaaagatga
     8101 ataaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg ttgcagatac
     8161 cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg attttcttga
     8221 acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt ttattgaccc
     8281 gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagtcgaca gtcgactggt
     8341 cagtcatcag gatggcaaat ggcaggtcag ccacggtcca ttcgaaagct acgatcatct
     8401 cggtgaaact agctggtcat tgttagtgca agcagtgaac cactggcatg agccgaccgc
     8461 cgcgctgatg cgaccgttcc gtgaactgcc ggactggcgt attgatgacc tgatgatctc
     8521 tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg tgtttatcat
     8581 tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga aacagcactg
     8641 cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg aagagctgga
     8701 gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg cgctggaaaa
     8761 tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaactga ttagtggatt
     8821 tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc cggatgttcc
     8881 acctcgcgct catcctgcgg acgttctgcc gcaagagatg gataaactgc gtgagatgat
     8941 gctcgaattg atcaaccagc cggaacattt taagcaatgg tttggcgagt ttatatccca
     9001 gtcacgtcat gaactggata tcgcgccgcc agagccgcct tatcagccgg atgaaatcta
     9061 cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggg ggtctgcgcg tattgcgcat
     9121 tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc cggcactgga
     9181 tgcgctcgcc agcaacattg cgctgaccgc ggagaatttt ggcgatgcgc tggaagatcc
     9241 gtcattcctc gcgatgctcg cggcgctggt caatagcgga tactggttct tcgaagggta
     9301 agtttggatt taagccggat gcggcgctga ttgcgcctta tcgtgcctac atgcactgaa
     9361 cgcctgtagg cacgataagt atgtggacca gtgtcaatat aaaggtttca tcccggtaat
     9421 tggcagtcca catacgcatc gggcaatcac gacttccgtt gcgccgtcaa ctcggcaata
     9481 cggacgatca cctgcaccgc tttttccata ccttccagag tcacaaactc atgcttacca
     9541 tgatagttgt aaccgccagt gaacaggttc gggcacggta atcccataaa cgacaactgc
     9601 gcgccgtcgg taccgccgcg gatcggtttc agttccggtt caatatcgca gtcacgcatc
     9661 gcctgctggg cgatatcgag aatatgcgga tgctcaacca ctttctcgcg catattgtag
     9721 taactgtctt caatcaccag ttcaatgtag caatcaggat gtaacccttt gcccactttt
     9781 ttggcgatct ccatcatttt acgtttacgc gcttcaaact gtttacggtc gaaatcacgg
     9841 atgatgtagt gcatatcggc ccgttcaacg gtgcctttca tgctcgccag gtgatagaaa
     9901 ccttcatagc cttctgtcat ttccgggctt tcatccgccg gaacttccgc atgaatacgt
     9961 gccgccagcg acagcgcatt taccattact cctttcgccg tgcccgggtg aacattgtta
    10021 ccgacaattt tgatattgac cgacgcggcg ttgaagtttt caaactccag ttcgcctacg
    10081 ccaccaccat caacggtgta agcccagcgg gcatcgaagg cgtcaacatc aaaatgcttc
    10141 gcccctttgc ccacttcttc atccggggta aaggcgacgc gaatatcacc atgcggaatg
    10201 tttttttgtt gcaataccgc cagcgcggtc atgatttctg caatacctgc tttgtcatcg
    10261 gcacctaaca aggttttacc atcggtggta atcagcgtct gacccagtag ctgatgcagc
    10321 accgggaaca taaccggtga taaaacttca tcgccgatac ccagcgcaat atcgccaccg
    10381 cgatagtttt caacaatttg cggattcaca tttttgccgc tgcaatccgg tgaggtatcc
    10441 acatgagaaa taaagccaat cgccgggata tcgccaggga cgttagccgg taacgtcgcc
    10501 atcaaagtgc ccttctcact taaggtcaca ttgataagcc ccatctcttc gagctgctct
    10561 ttcagcagat gcaataactt ccattggcct tccgtgctgg gaacctgtct cacccctgcc
    10621 tttgattggg tatccagaga cacgtagttc aaaaatcgct caagtagttt atccatgtag
    10681 tcaccctcac tttttgtgac aacattatta agaagcaaga aaagacaaat attgcgtcag
    10741 gtcactttta gccctgcaag cgggaatatt tatcagcatt accttaatga atataaagct
    10801 aagccagtaa ttcacaacaa agattggcga ttcaagcggt ttgccgtaga attaccgccc
    10861 tcattaagag tcaccaaggt ggttaaccac aaaccccgca tcggtaagcc atccgttgcg
    10921 tttacatggg acagagtaaa aaattgaata aacaaccgag ttcgctttca ccgctggtgc
    10981 aattggcggg aattcgcaaa tgctttgatg gtaaagaggt cattccccag ctggatctga
    11041 ctatcaacaa tggcgagttc ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg
    11101 ttcttcgcct gattgcaggt ctggaaactg ttgattccgg acgcatcatg ttggataacg
    11161 aggacatcac ccacgttccg gcggaaaacc gctacgtgaa cactgttttc caaagctacg
    11221 cacttttccc ccacatgacc gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa
    11281 cccccgctga tgaaattacg ccccgcgtga tggaagccct gcggatggtg cagctggaaa
    11341 ccttcgctca acgcaaaccg catcagctct ctggtggtca acagcaacgc gtcgccattg
    11401 cccgcgcggt ggttaacaag cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg
    11461 attacaaact gcgcaagcaa atgcagaacg agctgaaagc gttacagcgt aagcttggta
    11521 ttacattcgt gttcgtaact cacgaccagg aagaagcact taccatgtcg gacaggatcg
    11581 tggtgatgcg cgatggtcgc attgagcaag acggcacgcc gcgtgaaatc tacgaagagc
    11641 cgaaaaacct gtttgttgcc gggtttattg gcgaaatcaa tatgtttaac gccactgtca
    11701 tcgaacgtct cgacgagcag cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct
    11761 acgttaactt caccgttgaa ccggggcaaa aactgcatgt tctgctgcgc ccggaagact
    11821 tacgtgttga agagattaac gacgacaacc acgccgaagg gctgattggt tacgttcgcg
    11881 agcgtaacta caaaggcatg acgctggagt cggttgttga gctggaaaat ggcaagatgg
    11941 tgatggtcag cgaattcttc aatgaagacg atcctgactt tgaccactct ctcgaccaaa
    12001 aaatggccat taattgggta gaaagctggg aggtcgtact ggctgatgaa gaacacaagt
    12061 aagttccaga atgtagtgat tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg
    12121 cccaacctga tgatcattgg caccagcttt ttgacccgcg acgacgccag tttcgtcaaa
    12181 atggtcttta cgctggataa ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta
    12241 ttgcactcgc tgaatatggc gctgatcgcc accctcgcct gcctggtgct gggctatcct
    12301 tttgcctggt ttctggcgaa gctgccacac aaggtgcgtc cgctgctgct gtttctgctg
    12361 attgttccgt tctggaccaa ctcattgatt cgtatttacg ggctgaaaat tttcctcagc
    12421 accaaaggct atctcaacga gtttttgctc tggctgggcg ttatcgatac accaattcgc
    12481 atcatgttca cgcctagtgc ggtgattatc ggtctggttt acattctgct gccgtttatg
    12541 gtgatgccgc tgtactccag tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc
    12601 gatctcggtg ccagcaagtt gcagaccttt atccgcatca ttatcccact aacgatgccg
    12661 ggaattattg ccggatgtct gctggtgatg ctgccagcga tgggcctgtt ctatgtgtcc
    12721 gacctgatgg gcggtgcgaa aaacctgctg atcggtaacg tcatcaaggt acagttcctc
    12781 aatattcgtg actggccgtt tggtgcagcc accagcatta cgctgactat cgtaatgggc
    12841 ctgatgttgc tggtttactg gcgcgcttct cgtctgctga ataagaaggt ggaactcgaa
    12901 tgatcggtcg actgcttcgc ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc
    12961 caatcattat tttgattgtg aactccttta acagctcgcg ctttggcatc aactggcagg
    13021 gttttaccac caaatggtat agcctgctga tgaacaacga cagcctgtta caggcagcgc
    13081 agcattcact aacaatggcg gtgttttcgg cgacgtttgc tacgcttatc ggttcactga
    13141 cagcagttgc gctgtaccgt tatcgctttc gtggtaagcc gttcgttagc ggaatgctgt
    13201 ttgtggtgat gatgtcacca gatatcgtga tggcgatttc gctgctggtg ctgtttatgc
    13261 tgctgggtat tcagcttggc ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc
    13321 catttgtggt ggtgacagtg tattcgcgcc tgaaaggttt tgacgtgcga atgctggaag
    13381 cggcgaaaga tctcggtgcc agcgaattta ccattctgcg aaaaatcatt ctgcctctgg
    13441 caatgccagc ggtagcggcg ggctgggtgt taagctttac cctgtcgatg gacgacgtgg
    13501 tggtttcttc gtttgtcacc gggccgagtt atgaaattct gccgttaaaa atttattcga
    13561 tggtcaaagt cggcgtatcg ccggaagtta acgcgctggc aaccatatta ctggtgctgt
    13621 cgctggtgat ggtaattgcc agccagctta ttgctcgtga taaaacgaaa ggtaacacag
    13681 gggacgttaa atgaaaaaat ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg
    13741 catgagcgcc gctcacgccg atgacaacaa cacgctgtat ttctacaact ggaccgagta
    13801 cgtgccgcca ggactgcttg aacagttcac caaagaaacc ggtattaagg ttatctattc
    13861 gacttacgag tcgaacgaaa ccatgtacgc gaagctgaaa acgtacaaag acggtgccta
    13921 tgacctggtg gttccttcaa cctattacgt cgataaaatg cgtaaagaag ggatgatcca
    13981 gaagatcgac aagtcgaagt taagcaattt cagcaacctc gatccagaca tgctcaacaa
    14041 gccgtttgac ccgaataacg actattccat tccgtatatc tggggtgcaa cggcgattgg
    14101 cgttaacggt gatgcggtgg atccgaaatc tgtcaccagc tgggccgatc tgtggaagcc
    14161 tgagtacaaa ggcagcctgc tgctgaccga cgatgcccgt gaagtgttcc agatggcgct
    14221 gcgtaagctg ggctactccg gtaacaccac cgatccgaaa gagattgaag ctgcatacaa
    14281 cgagctgaaa aaactgatgc caaacgtggc ggcgtttaac tccgataacc ccgctaaccc
    14341 gtatatggaa ggcgaagtta acctcggcat gatctggaac ggttctgctt tcgttgcacg
    14401 ccaggcgggt acgccaattg atgtggtgtg gccgaaagaa ggcggcattt tctggatgga
    14461 cagcctggcg atcccggcaa atgccaaaaa caaagaaggc gcactgaaat tgatcaactt
    14521 cctgctgcgc ccggatgtgg caaaacaggt tgctgaaact atcggttatc caacgccaaa
    14581 ccttgcggcg cgtaagctgt taagtccgga agtggcgaac gataaaacgc tctacccgga
    14641 tgctgaaacc attaaaaatg gtgaatggca gaatgacgtt ggcgcagcca gcagcattta
    14701 tgaagagtat tatcagaagc tgaaagcagg acgttaattc agcaagcggg cggtggtatt
    14761 accgcccgtt ttttatttag caaggatgtg gctaatgagc caggacagta gagttttctt
    14821 tcgtatattc ttcggtatcg gtctggtact gatcctcatt tctgttgtta tcttttataa
    14881 ccagttcacc tatagcaaag acgcgattca tactgaaggc gtcattgttg ataccgtctg
    14941 gcactccagt cattctcatc gcacgggtaa aaatggttcg tggtatcccg tcgttgcctt
    15001 tcgtccgacg ccagattaca cgctaatttt caattcaagt attggcagcg atttttatga
    15061 agacagtgaa ggcgataaag ttaatgttta ttattctcca ggacatcctg aaaaggctga
    15121 gataaataac ccgtgggtta atttttttaa atgggggttt atcggcatta tgggcgttat
    15181 ttttatctcc gtcggtttat taatcagtat gccctactct aaaaagacac gcaggaaacg
    15241 gaagtcccgc ccatgaaaaa aatcataata ttgctcagtt tactgatact cctgcctctg
    15301 acggtaacca gtaagccatt gattccaata atgaagactc tttttacgga tgttaccggt
    15361 actgtaccgg atgccgaaga gatcgcgcgt aaagccgaac tttttcgtca acaaaccggg
    15421 attgcgccgt ttatcgtggt tgtacctggc gttaatgaag caagcttcag gcaaaatggc
    15481 aaagcgatgc tggcgcatgc ggcatcttca atgagtaatg taaaaggaag tgttctgtta
    15541 ctttttacta cccgcgagcc acggttaatt atgataacca acggccaggt tgaaagcagc
    15601 atggacgata aacatctcgg ccttctggta gaaaatcaca ctctggctta tttacatgca
    15661 gatctctggt atcagggaat caataatgca ttggctgttt tacaagcaca gatattaaaa
    15721 caaccgacgc cgccactgac atattatccg catcccggac aacagcagga gaatgatctt
    15781 cccggcagta ccacgacatt aggccttttt gcctgggcag ttgcattcat cgtctttgct
    15841 gcatttttta actacacaac gcgcctttat tatgcgttaa aatttgcagt ggctatggct
    15901 gttgccaata tgggctatca ggctttatgt ttgtatatcg ataacagctt tgcgattacc
    15961 agaatatcgc cgctctgggc agggctgatt ggtgtctgta cgtttattgc cgcgttacta
    16021 tggacgagta aacggtaaaa gaggcagtaa agttgccgga tgcggcgcga acgccttatc
    16081 cggcccacaa aacccgcaaa ttcaattaat tgcgcccctt cacaggcctg acaagcgtag
    16141 cgcatcaggc aatttttccc gctttataat cccttcagca acttttcaac aaactctggc
    16201 accacctggc ttgccggacc gtaatatttc tcggcaaatt cattaccaac ctgactcggt
    16261 tcaagattca gttccacggt gtgcgcgcca tgcagtttcg cttcgtgaac aaacccagcc
    16321 gccggataaa catgccccga agtaccaata gcaatgaaaa tatcggccat cgacaacgcc
    16381 atataaattt catccatgcc gagtggcatt tcgccaaacc acactacgtg cgggcgcaag
    16441 ggtgccggga actggcaaca atggcattta tcttctgggg taacatctcc ggtccagtcg
    16501 agaacctgac cactttgcga acagcgaact ttcagcagtt ccccatgcat atgaatgaca
    16561 ttggtattac ctgcgcgttc atgcaggttg tcgatattct gcgtcaccag caaaaagcga
    16621 tcgccgaggg cgtcttgcag tttcgccagc gcaagatgcg cggcgttagg ctgaatttct
    16681 ggctgctgca gctgtcgacg acgggcgtta taaaacgttt gcaccagttc aggatcgcga
    16741 tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag gccatctgcg
    16801 gcgcgaaagg tacgaatacc tgattccgca gaaatccctg cccctgtcag tacgagtact
    16861 cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg ctgacgcaaa
    16921 cgctcgcgca gacggcgttt atttttacga aaacgactta accgatgacc ccgacgcgac
    16981 agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc acgcattcct
    17041 cccgcatcac cgtggcgcgc gcgttcaatg cgcggaacac gagctacagg taagagatga
    17101 cgaggcagcc tgtccgccag ttgcgttgtg attgccggga aattcgataa gccaccacca
    17161 atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagacaaac cgctaataaa
    17221 tccagataac gctcaacgtg cgcccttgcc tgctcatcac cttgatcata aagcgcaata
    17281 atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc aaaaccgcga
    17341 ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg taacgggaaa
    17401 tccagcccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa ctcgccggta
    17461 atatagcttt taccggtaat gggtttaccg ttgaaaatca gcccgccgcc aacgccggtg
    17521 ccgagaatca accccatcac cagtggatat tgagtaaatt catcatccca tgcttcagaa
    17581 agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg tgcgctcagg
    17641 tcggcacgca gcggtttacc gctggcagca gggacattgg cggcatacag cgtaccatct
    17701 tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc aaaacgccgg
    17761 tcagcttcgg ctaccagctc gcacactgca tctaaaaatg cgtcatagct gtcacgcggt
    17821 gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc aagcgcaatt
    17881 tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa tgtactgcct
    17941 ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgtcgcg ccggatacca
    18001 acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt ccagccagtg
    18061 caattccgat ggcaggaagt caataaaata gatatcgctg gagaggaact gatgaccgat
    18121 caacttttca atccactcaa taatcggggt aagttgcagt gaaacaacta cgccgataat
    18181 cacaccacac aggctaccga acagccctgc cagcaatcca taccagacaa agatggcgcg
    18241 aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac tcttgtcttt
    18301 caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca ccagtaccat
    18361 cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc cgtaagtacc
    18421 aatccagctt ttaatataaa catagctgtt ggtcacttca cccgcatcgc gtaccagctt
    18481 attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg aacccatatc
    18541 aagatattgc tgggcatccg ccagcgggat catggcaaaa ctgtgatcga gttgaccact
    18601 caactgcaaa atatcggcaa tgtgcaaacg cacacgtttt ggctgcatca gtttatgctc
    18661 gggattcgag ttggggatca taatcgacac ccaatcgccc tgcttcactt tcagcgcatc
    18721 cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc gccaggcatc
    18781 cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgcgggt taacgccctt
    18841 cacctggatt gcgcgcagat tggcaccact ttctaccagt ccggtgaaat tgatatacgg
    18901 cgcagcggcg gcaatacctg gcactttttg cacgttatcc agggcttcct gccagttagt
    18961 ccacggttga tccaccgcct ctatttcgcc atgcggcacc accgccagaa tgcggttatt
    19021 cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg ccacgccaag
    19081 ggcaatgcca atggtagaaa tgacggagat cagcgacacc atgccgccgc gccgccgtcc
    19141 gcggctaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc cgcccccatc
    19201 aggctcagtt ccgccgtcag acgaccatca cgcatctcca gttggcggct catacgtttt
    19261 gccagttgca ggtcgtgagt aaccaccagg aaggcggtgc cctgcaagcg attcaattct
    19321 ccaagcaact ggaagatgct gtcggcgtta cgtgcatcga ggttaccagt aggttcatcc
    19381 gccagcacca gccgcgggtt attgaccagc gcacgggcaa tcgccacgcg ctggcgttcg
    19441 ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac cgcttttaac
    19501 atctccagcg cacggctgtt gatttcagcg ggctttttct tgccaatcag cagcggcata
    19561 gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata aataaagccc
    19621 agcttctggt tgcgcagttc tgctttcgcc gccgacgaca gtttgctcat cggctgaccg
    19681 ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg cagcaaggta
    19741 cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc cacgctgaaa
    19801 ctgacattgt gcagcacatc ggtttgcaca ctgccttcct gatagcgttt gcacaggttg
    19861 tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg tggcggcagc
    19921 gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca gcgcaataac
    19981 aatgacctgt aaaggttcga tagcgaccgg cagcgccgcg ccatcaagca ggacgccgat
    20041 tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg ctccgaggat
    20101 cgcaccgata atcccggcgc tggccccttg caccataaag accatcatga tttgtcgcgg
    20161 agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca ccattagccc
    20221 tagcgaggta ataatgttaa acgccgctac ggcaacaatc aggctcagca gcaagcccat
    20281 catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac gccagtcctg
    20341 ccatttgctg ccttcaggca gtttttgttg acttaatgag tcgactttca gcggctcatc
    20401 cagccacaaa cgccagccgg taatattgcc tgccggataa cgcatcagac gcgaggcatc
    20461 ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga aagtgccaat
    20521 cacattgaac aggcgctggc ttggaatacg ccccatcggc gtgaactggc tggcagatgg
    20581 caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcca gctgttcacc
    20641 gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga ccagatacgg
    20701 cgttagtgga tctttttgtg ccgggtcgat acccagcatc accccgaccg ccacgctgcg
    20761 cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa cgccgtccag
    20821 tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag aagagagaat
    20881 tgcctgtggc atcaggccaa ggatgttgtt ttgtagctcg cgctcaaagc cgttcatcac
    20941 tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag aaagccagga
    21001 gacgaaacga ccgaagcgat ccgctgcacg cccacgcatg taacgcaggc caataaatag
    21061 agcgacaggt tggtacatga aatccgtctg gttgctgtta gcaaagacac gagtatataa
    21121 gcgaaagcgg gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    21181 tgccagcaaa tcaatcggct aaagggcctc attttctcat cggccttgta gcctgatctt
    21241 tatctgaaaa ataaccaaat acagacagat acgcattaca ccacgcgact ggcccctcag
    21301 gatcaacagc atgttaaata aaaagagcgt gttgtcctga tgaaacaaaa agagctatgg
    21361 attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc ggtcattacc
    21421 ttttatccgc atctcaccac ttttcagcat ccgttatcgg aagtcctgag caaatgctgg
    21481 atctatttca atctttacct tgcacccttt cgtatgccgg tttttttctt tatatccggc
    21541 tatttgattc gccgttatat cgacagcgta ccgtggggaa attgtctcga taaacggatc
    21601 tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc gttaagtgcg
    21661 ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc cgcgtatgcc
    21721 gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagttt gtggtatctg
    21781 tatgcgttaa ttgtttattt cgtggtatgt aaaattttta gccgtctggc gctgccacta
    21841 ttcgccttgt ttgtactgat gagtgtggcg gttaatttcg ttcccacgcc gtggtgggga
    21901 atgaacagtg tgatccgcaa tttgccttat tacagccttg gcgcatggtt tggcgcaaca
    21961 ttaatgacct acgttaaaga ggtgccgttg cgccgccatc tgctgatgac ttctttgctg
    22021 accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc gctggtatcg
    22081 attgtggtga tcatgaagct gttttatcaa tacgagcaac gtttcggtat gcgctccacc
    22141 agcctgctga atgtgattgg caccaacacc attgctatct acaccaccca tcgcattctg
    22201 gtagagatat tcagcttaac tctgcttgcg ccaatgaacg cggcacgctg gtcgccgcaa
    22261 gtcgaactga cactcctgat ggtttatccc tttgtcagtt tgttcatctg tactgttgcg
    22321 ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt ctccccgcct
    22381 tcactgcccg ctgccgtcag ttactcccgc taagccatcg ccccagccgg ggcgattcaa
    22441 tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag atatgccccc
    22501 atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac aatatcgtta
    22561 tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg gcgctgcctg
    22621 cgcgacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac ttattgcacc
    22681 agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagtttaccg atcagatggt
    22741 gatgaatctg gcggactggg aaacccttcc ctacgacagt ttttcgcctc atcaggacat
    22801 tatctcctcg cgcctttcca ccctttacca gctaccgacc atgcagcgtg gcgtactgat
    22861 tgttccggtg aatacgctta tgcagcgcgt ttgcccgcac agttttctcc acggtcatgc
    22921 gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc aactggacag
    22981 cgccggttat cgccatgttg accaggtaat ggagcacggc gaatacgcca cgcgcggcgc
    23041 gttgctggat ctcttcccga tgggcagtga gctgccttat cgtcttgatt tctttgatga
    23101 tgaaatcgac agcctgcgag tgtttgacgt cgatagccag cgcacgctgg aggaagtaga
    23161 agagatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa ttgaactgtt
    23221 tcgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata tttatcagca
    23281 agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccgctat tcttcagcga
    23341 accgctgccg ccactgttca gttatttccc tgccaatacg ctgttggtga atactggcga
    23401 tctggaaaac agtgccgaac gtttccaggc tgacacgctg gcgcgttttg aaaatcgcgg
    23461 cgtcgatccg atgcgcccac tgttgccacc acaatcgctc tggctgcggg tagacgagct
    23521 cttttcagag ctgaaaaact ggccgcgtgt gcagctaaaa actgaacatt taccgacaaa
    23581 agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc aggcacaaca
    23641 aaaagcaccg ctggatgcgc tgcgtaagtt cctcgagtct ttcgacggtc cggtggtgtt
    23701 ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcgc gaattaaaat
    23761 tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt atttgatgat
    23821 tggcgctgcc gaacatggtt ttgtcgatac ggtacgtaat ctggcgctga tttgcgaaag
    23881 cgatctgctc ggtgaacgcg ttgcccgtcg tcgtcaggat tctcgccgcg ccatcaaccc
    23941 cgatacactg atccgtaacc tcgcggagct gcatattggt cagccggtgg tccatctgga
    24001 gcacggtgtc gggcgctatg ccggaatgac cacgctggaa gcgggcggta ttactggcga
    24061 gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt cgtcactgca
    24121 tctgattagc cgttacgcgg gtggcgcgga agaaaacgcc ccgctgcata aacttggcgg
    24181 cgatgcctgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg tggcagcaga
    24241 attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta aacacgatcg
    24301 cgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg atcaggcaca
    24361 ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc gtctggtgtg
    24421 cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc tggcagtaga
    24481 taaccacaag caggtggcgg tgctggtgcc aaccaccctt ctcgcgcagc agcattacga
    24541 caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct cccgtttccg
    24601 cagcgccaaa gagcagacgc aaatccttgc ggaagtagcg gaagggaaaa tcgatattct
    24661 gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag gcctgctgat
    24721 tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag cgatgcgcgc
    24781 gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga atatggcaat
    24841 gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc tggcagttaa
    24901 aacctttgtc cgtgagtatg acagtctggt ggtccgggag gcgatcctgc gtgaaatttt
    24961 gcgcggagga caggtttatt atctctacaa tgatgtggaa aacatccaga aagccgccga
    25021 acggctggca gaactggtac ctgaagcgcg gattgccatc ggtcacgggc aaatgcgtga
    25081 gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg tgctggtttg
    25141 taccaccatt atcgaaaccg ggatcgacat tcctaccgcc aacaccatta tcattgaacg
    25201 cgcggatcac ttcggtctgg cacagttgca ccagttacgc ggtcgcgtcg ggcgttcgca
    25261 ccaccaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta ccgatgcgca
    25321 aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg cgctggcaac
    25381 gcacgatctg gagatccgcg gcgcaggtga actgcttggc gaagaacaaa gcggctcaat
    25441 ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgctgtcg atgcgctgaa
    25501 agccggacgc gagccgtcgc tggaagatct caccagtcag caaacagaag tcgagctgcg
    25561 gatgccgtcg ctattgcccg atgatttcat ccctgacgtg aacacgcgtc tgtcgttcta
    25621 caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag tcgagcttat
    25681 cgatcgcttc ggcctgctgc cggatccggc gcgtaccctg ctggatattg cccgtctgcg
    25741 ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag gcggcgtgat
    25801 cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc tgcaaaaaca
    25861 gccgcagcat tatcgcctcg atggtccgac gcgcctgaag tttattcagg atttgagtga
    25921 gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag agaacgcgat
    25981 cgcttaatgg taaatcgggg gcgtttctgc gcccccatct gcaacattta caaattcttt
    26041 gcacttccct gcactatccg acactgtcac catccataat tcaggcttat ctgtttatta
    26101 caataacctt atatttatta tggatttttg gtgatgatca aaacgcgttt ttctcgctgg
    26161 ctaacgtttt ttacgttcgc cgctgccgtg gcgctggcgc taccggcaaa agccaacacc
    26221 tggccgctgc cgccagcggg cagtcgtctg gttggcgaaa acaaatttca tgtggtggaa
    26281 aatgacggtg gttctctgga agccatcgcc aaaaaatata acgtcggctt tctcgctctg
    26341 ttacaggcta accccggcgt tgatccttac gtaccgcgcg cgggcagcgt gttaacgatc
    26401 ccgttgcaaa ccctacttcc agatgcgccg cgcgaaggca ttgtgatcaa tattgcggaa
    26461 ctgcgtctct attactaccc gccgggtaaa aattcggtaa ccgtgtatcc catcggcatt
    26521 ggtcagttag gtggtgacac gctgacgccg acaatggtga ccaccgtttc agacaaacgt
    26581 gcaaacccaa cctggacgcc aacggcaaac atccgcgccc gttataaagc acagggaatt
    26641 gagttgcctg cggtagttcc ggctggaccg gataatccaa tgggccatca tgcgattcgc
    26701 ctggcggcct atggcggcgt ttatttgctc cacggtacga acgccgattt cggcattggc
    26761 atgcgggtaa gttctggctg tattcgtctg cgggatgacg atatcaaaac actctttagc
    26821 caggtcaccc caggcaccaa agtgaatatc atcaacactc cgataaaagt ctctgccgaa
    26881 ccaaacggtg cgcgtctggt tgaagtacat cagccgctgt cagagaagat tgatgacgat
    26941 ccgcagctgc tgccaattac gctgaatagc gcaatgcaat catttaaaga tgcagcacaa
    27001 actgacgctg aagtgatgca acatgtgatg gatgtccgtt ccgggatgcc ggtggatgtc
    27061 cgccgtcatc aagtgagccc acaaactctg taaagctcgg ataaaaaaat ccccgcgaca
    27121 cggcatgtcg cggggaaaaa aatcagcagt agcaggcatt aatgagggtt aatgcttatt
    27181 tataaattac tgctgttcca tggagggtat tcggaccggt tacagaagta atacggaaag
    27241 attttgcgcc catctcatcc gctttttgcg ccagctgctc ttccagcgat cccagatttg
    27301 tccccgcgtt agcactgata gtaccgactt tttgttggcc ttctggcgtt gactgaactt
    27361 cgacagccgc aaagctggca aatgacattg agcttaaaat cgccgcagcg atgagggttt
    27421 ttacgttttt cataatagtg gccttatgca gatgaatgac gtcgcaagca attacttaac
    27481 gatcgataac taaatgatag atgtgatctg gatcacatac aagatatttt ttataacaat
    27541 cattaattaa tttaaaaatc tttaaatttc agttaaatag aaagcaatta tttttatggt
    27601 ttgtggactg gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag
    27661 gtacaacggc atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gcggccgccc
    27721 aaaagtgttc gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg
    27781 ttatgaagcg acttctcttg cggacctcgt cgaagcgacc ggagccaaag cgcccacgct
    27841 atacgcggaa tttaccaaca aagaggggtt attccgcgcc gtactcgacc gctatatcga
    27901 tcgttttgcc gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc
    27961 gctggctgac tattttgctg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg
    28021 ctgcttcatg atcaacaact gcaccaccct ctccccagat tcaggagata tcgccaatac
    28081 gctgaaatca cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca
    28141 agcgcgcggg gaaatcccga cccactgtga cgtgacgcat ctggcagaat tccttaattg
    28201 tattattcag gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca
    28261 gattgcccga acgactttgc gtttatggcc cgaactggtg aaataaggcg caaaaaaagc
    28321 ccctcagcct gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta
    28381 ttcaaaatca gttgcccgtt gctatctagc gggatctgcg tacccggatc gcggtccatg
    28441 cggattttgc cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct
    28501 gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg
    28561 agagagccct ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtagcg
    28621 acatctttac cgcgaccacc accaaactga tgccctatca cgccgccagc aacagcgccg
    28681 agcaccgacc cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg
    28741 ttacgacact cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc
    28801 tgagcgtatt gcgggccacg ttcaaacacg ttcagactgg ccactgccgc tacgcccaac
    28861 gcagctgcga caccaatccc gatacccgcc aacattgatt tattcacggg aatacctcct
    28921 tctttgcagt tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg
    28981 aaaagcgcgg gaatggctgg aaatacttag ggattcggag gagtctgaag gaatgcctga
    29041 tgcgacactt aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggataa
    29101 ggcgtttatg ccccatccgc cagtacaagc cgattaatgc aacttcaaac gtggacggat
    29161 aacgcggtta atactcccca ccagcatcat taatccagtt ttaaagtaac catgcagcgc
    29221 aatctgatgc attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat
    29281 cattgagccg cgtgtcaggt tacccatcag gctaccgacg gtggaaaagt tcgacagcga
    29341 taccagcgaa ccgtgatctt tatactgata atttttcagc ggcttaccgt tcatctgcgc
    29401 cagaatgttg ttcattgcgc aggttgccat ctgatgtgcg gcctgagcgc gcggcggaac
    29461 aaagccccct tccggacgcg ggcatgacgc gcagtcgcca atagcgtata tgtctggatc
    29521 gcgagtggtt tgcagcgtcg gttccaccac cagctggttg atacggttgg tttcaagacc
    29581 accgatatct ttcaggaagt ctggcgcttt gatcccggct gcccacacca tcagatcagc
    29641 ctcaatatat tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt
    29701 ttgcgtcagc acgcgaacgc caagtttcgt taactcacta tgggccgcag cagagatacg
    29761 tggcggcaat gcaggcaaaa tacgttcgcc cgcttctacc agcgttacgt tcagggcttc
    29821 gttggtcaga cctttataac cgtaactgtg cagttgcttg accgcgttgt gcaattcagc
    29881 ggagagttct acacccgtcg cgccgccgcc gacaatcgca atgtttactt tgccattcgc
    29941 gcccaggttg gcggagtatt tcaggaacag attcagcatc tcctggtgga agcgacgcgc
    30001 ctggtgcggg ttatcgagga aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt
    30061 ggaggtgcta cccagcgcca ttaccagggt gtcataggcg attttacgtt ccggaaccag
    30121 caattcacct ttttcgtcgc gcagttctgc aatagtgatt gttttcgctt cacgatcaat
    30181 atcaattacg gaacccagct ggaactggaa accatgattg cgcgcatggg ccagatagct
    30241 caacgcatcg acgccttcat caagcgagcc agtcgccact tcgtgcagca gcggtttcca
    30301 caggtggctg tggttacgat cgaccagcgt aattttggct tttttcttgc gtcccagctt
    30361 atgccccagc tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatttt
    30421 tttcaatggc gtag
//
LOCUS       DAZUCC010000036        30222 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZUCC010000036 DAZUCC010000000
VERSION     DAZUCC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.036"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(86..1777)
                     /gene="fruA"
                     /locus_tag="ACHY09_001460"
     CDS             complement(86..1777)
                     /gene="fruA"
                     /locus_tag="ACHY09_001460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFS3884450.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVATSG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKEGAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYISFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(1794..2732)
                     /gene="fruK"
                     /locus_tag="ACHY09_001461"
     CDS             complement(1794..2732)
                     /gene="fruK"
                     /locus_tag="ACHY09_001461"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS3884451.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFDVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(2732..3862)
                     /gene="fruB"
                     /locus_tag="ACHY09_001462"
     CDS             complement(2732..3862)
                     /gene="fruB"
                     /locus_tag="ACHY09_001462"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFS3884452.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4230..5411
                     /gene="setB"
                     /locus_tag="ACHY09_001463"
     CDS             4230..5411
                     /gene="setB"
                     /locus_tag="ACHY09_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFS3884453.1"
                     /translation="MHNSPAVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVTVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(5408..5662)
                     /gene="yeiW"
                     /locus_tag="ACHY09_001464"
     CDS             complement(5408..5662)
                     /gene="yeiW"
                     /locus_tag="ACHY09_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFS3884454.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            5817..6389
                     /gene="yeiP"
                     /locus_tag="ACHY09_001465"
     CDS             5817..6389
                     /gene="yeiP"
                     /locus_tag="ACHY09_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFS3884455.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6612..8078
                     /gene="yeiQ"
                     /locus_tag="ACHY09_001466"
     CDS             6612..8078
                     /gene="yeiQ"
                     /locus_tag="ACHY09_001466"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS3884456.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQSGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARALMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLARE
                     TDWSLLALGVAGWMRYVSGVDDAGNTIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            8196..9182
                     /gene="yeiR"
                     /locus_tag="ACHY09_001467"
     CDS             8196..9182
                     /gene="yeiR"
                     /locus_tag="ACHY09_001467"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFS3884457.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHGVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVMRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9221..9934
                     /gene="lpxT"
                     /locus_tag="ACHY09_001468"
     CDS             9221..9934
                     /gene="lpxT"
                     /locus_tag="ACHY09_001468"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFS3884458.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10346..10912
                     /gene="mepS"
                     /locus_tag="ACHY09_001469"
     CDS             10346..10912
                     /gene="mepS"
                     /locus_tag="ACHY09_001469"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFS3884459.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11093..12649
                     /gene="pdeN"
                     /locus_tag="ACHY09_001470"
     CDS             11093..12649
                     /gene="pdeN"
                     /locus_tag="ACHY09_001470"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFS3884460.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFTDLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLKELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            12731..14545
                     /gene="yejA"
                     /locus_tag="ACHY09_001471"
     CDS             12731..14545
                     /gene="yejA"
                     /locus_tag="ACHY09_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS3884461.1"
                     /translation="MIVRILLLFIALFTFGAQAQTIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNAGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRLEND
                     AKNWVTRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            14546..15640
                     /gene="yejB"
                     /locus_tag="ACHY09_001472"
     CDS             14546..15640
                     /gene="yejB"
                     /locus_tag="ACHY09_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFS3884462.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNTGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            15640..16665
                     /gene="yejE"
                     /locus_tag="ACHY09_001473"
     CDS             15640..16665
                     /gene="yejE"
                     /locus_tag="ACHY09_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3884463.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            16667..18256
                     /gene="yejF"
                     /locus_tag="ACHY09_001474"
     CDS             16667..18256
                     /gene="yejF"
                     /locus_tag="ACHY09_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFS3884464.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIVFDGKPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18260..18604)
                     /gene="yejG"
                     /locus_tag="ACHY09_001475"
     CDS             complement(18260..18604)
                     /gene="yejG"
                     /locus_tag="ACHY09_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFS3884465.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(18938..20128)
                     /gene="bcr"
                     /locus_tag="ACHY09_001476"
     CDS             complement(18938..20128)
                     /gene="bcr"
                     /locus_tag="ACHY09_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFS3884466.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(20156..20851)
                     /gene="rsuA"
                     /locus_tag="ACHY09_001477"
     CDS             complement(20156..20851)
                     /gene="rsuA"
                     /locus_tag="ACHY09_001477"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFS3884467.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21001..22761
                     /gene="radD"
                     /locus_tag="ACHY09_001478"
     CDS             21001..22761
                     /gene="radD"
                     /locus_tag="ACHY09_001478"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS3884468.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAVTRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            22886..23170
                     /gene="rplY"
                     /locus_tag="ACHY09_001479"
     CDS             22886..23170
                     /gene="rplY"
                     /locus_tag="ACHY09_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFS3884469.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(23309..24316)
                     /gene="yejK"
                     /locus_tag="ACHY09_001480"
     CDS             complement(23309..24316)
                     /gene="yejK"
                     /locus_tag="ACHY09_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFS3884470.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24498..24725
                     /gene="yejL"
                     /locus_tag="ACHY09_001481"
     CDS             24498..24725
                     /gene="yejL"
                     /locus_tag="ACHY09_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFS3884471.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            24745..26514
                     /gene="yejM"
                     /locus_tag="ACHY09_001482"
     CDS             24745..26514
                     /gene="yejM"
                     /locus_tag="ACHY09_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFS3884472.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTAR
                     RFLEKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYS
                     RFEKQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALI
                     TALNQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQE
                     DNRWFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVII
                     TAGRGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHV
                     STPASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERV
                     KDEKPQLSLLLQVLTDEKRFIAN"
     gene            26589..26665
                     /locus_tag="ACHY09_001483"
     tRNA            26589..26665
                     /locus_tag="ACHY09_001483"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26623..26625,aa:Pro,seq:ggg)
     gene            complement(26791..27550)
                     /locus_tag="ACHY09_001484"
                     /pseudo
     CDS             complement(26791..27550)
                     /locus_tag="ACHY09_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            27870..28517
                     /gene="narP"
                     /locus_tag="ACHY09_001485"
     CDS             27870..28517
                     /gene="narP"
                     /locus_tag="ACHY09_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFS3884473.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(28552..29604)
                     /gene="ccmH"
                     /locus_tag="ACHY09_001486"
     CDS             complement(28552..29604)
                     /gene="ccmH"
                     /locus_tag="ACHY09_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFS3884474.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKAAPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(29601..30158)
                     /gene="dsbE"
                     /locus_tag="ACHY09_001487"
     CDS             complement(29601..30158)
                     /gene="dsbE"
                     /locus_tag="ACHY09_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFS3884475.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKL
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT         7248 a         7932 c         7517 g         7525 t
ORIGIN      
        1 aaattacatt cacaaactgg tcattccctt tgcgctcatc aaatgttaca gggcaggaaa
       61 tttctgccct gtaacacacc ttttattacg ctgctttcgc tactgcgtcc acttccggac
      121 gtttcaggaa ggcataggcc aaacccgcca ccagcgtacc ggcaataatt gctaccaggt
      181 aacccagtac cggcgtaata gcgccaggga tcagcagaac aaacagacca ccgtgcggtg
      241 ccataagttt cgcaccaatc gccattgaga ttgcgccagt cagcgcccca ccaacgatac
      301 aacacggcag cacacgcatc ggatcacgag cagcaaacgg aattgcacct tccgaaataa
      361 agcacagacc aagaaccaga gcggctttgc caccttcctg ctgcgctttg tcgaatttgc
      421 gacgcgccac cattgttgcc agacccattg ccagcggtgg caccatacct gccgccataa
      481 tcgccgccat cgggccatag gtttgagtac tcagcagacc cacaccgaat gcgtacgctg
      541 ctttatttac cggaccgccc atgtcagtac acatcatgcc accgaggatc gcccccagca
      601 gaaccgcatt cgcagtcccc atggtctgca gccagtgagt caacccttcg agaatgccag
      661 caaccggttt accgatcagg tagatcatcg ccagaccgac caccagactg gaaattagcg
      721 gaatgatcag gatcggtttc agcgcctcca tactctgtgg cagtttcagt tgcgtactga
      781 ttaacttcgc aatgtaacca gccaggaagc ccgcaataat acccccaatg aagccagaac
      841 cggtgctgac cgccagcata cccccaatca gacccggcgt gagacccgga cgatcggcaa
      901 tggagaagga aatgtaacct gccagtaccg gcaccatcag cgcaaaggct gaaccaccac
      961 caatctgcat cagcgccgct gccaacgtac ccggctcttt aaacgcttcg ataccaaaag
     1021 caaaagaaag cgcgatacac agaccacctg caacgaccat cggcagcatg taagagacgc
     1081 ccgtcagcaa gtgacggtaa gcgcctgcac cctctttctt accttcagtg gtcgctgttt
     1141 gagctttgcc cgccggttca tacggcgttg cttcagcaac cgctttatcc agttcctgcg
     1201 cggttttctt cagcgccaga ccggtagagg tgcgatacat cggtttacca gcaaatttcg
     1261 ccagatccac ttcgatatct gccgccacaa tcaccagatc cgctgcggct acttcttcag
     1321 gggtgatcgc attacccgcg ccaacagaac cacgggtttc aactttcacc caccagccac
     1381 gttttttcgc ttcggtttca atggcttcag ccgccataaa tgtgtgtgct acgccagtcg
     1441 ggcaagctgt caccgcaact acgcgtttcg gaccgctggt ggcaactggt gctgtcgcag
     1501 cgaccggtgc agtgtaaggt ttcgcatggc ctttggcttc actcaggaac agctcagggt
     1561 gcgcaactgc ccgggaaata tcgcccagcc agacattttt accgttcagc gcgctgtcgt
     1621 tcgggatgga atcaccgaga acaatcgcca tttcagcgtc gttcggattg tcgatgattt
     1681 ccagttttgc ttttcgcgcc gccgcgccca gcagggtctt cgccatatag gcgcgtgcct
     1741 gaccgagatt agcgtcaata atcagcagcg ttttcattat gcctctcctg ctgtcagtta
     1801 aaaggttgta agtcgacgcg cgccatcatt gcggccaact gcggacgatc ggtaataccc
     1861 acattgcttt gacttaccgc cagggctgca acagctgtcg ccagacgcag tgtgtgttca
     1921 ctggattcac gcatcagcaa gccataaatc aggccaccaa ccatagaatc ccctgcgcca
     1981 acggtgctta cgacatcgac tgacggtggt ttggcgatcc attcgccgga ggcattaacc
     2041 caaagcgcgc cttcggcacc cagtgaaata acgacatgcg cgataccttg ttcacgcagc
     2101 gcatgcgcag cttcaatcac atctttcatt tcaggcagtt tacggcctgc ccagatttcc
     2161 agctcgcggc ggttaggttt caccagccac ggtgccgctt tcaaacctgc tactaacgct
     2221 tcacggctac tatcaaagat aatgcaagga cactgactac gcaggcgagt catccagtcg
     2281 gtgaacgctt ccgggctgac gcctgacggt aagcttccgc tgacacagac catatcgaac
     2341 tgaccgagcc agctcagaga atcagtcaca aagcgttccc agtcggcggg ggtaacgtca
     2401 aaacccgaga agttgaagtc ggtcacttcg ccgtcttttt ccgtcagctt aacgttaatt
     2461 cgggtgcgcc cctgtacaac ctggaaacgg ttggcaatgc ccagctcgct gaacagttgc
     2521 tgaaaaccat cctgattgtc tttaccgagg aagccaccaa cggtgacatc aattcccagg
     2581 tcttttaata ctttggccac gttgatgcct ttacccgccg catgcagacc ggtggttttc
     2641 accaggttca cttcgccgcg ttcaatttcc gggcagaaac caacaaggtc ataagccgga
     2701 ttaagggtga tagtagcaac acgtctgctc attatgcgcc ctccccaaga ccagcagcga
     2761 tagcgtcgcc gattgctttc agcgcctgtt cagcatctgc accctgggcg gtaaagcgta
     2821 ggcgatgacc tttcttaacg ccaagtgcca caactttcat cagactacgt ccgtttgccg
     2881 gtttgccggt accatcaagg tttgtcacgg taatatcact gttaaattgt ttaatggtat
     2941 tgaccagcat ggtacctgga cgagcatgca ggccgtgttc attgcgcacc acaaactccg
     3001 cgcttaacac gtcgtcggtc ggcgcatcat cgctggtcag cagcgccagc aacgttgccg
     3061 catccgcttt cagcaagcgg tcagctttat tgtcgagtaa caaatcagcg agacgcttaa
     3121 gaaccgcaat gggctgatcg tcattcatcg ccacactcac cagcatggct gccgtttcgc
     3181 cgtccacatc aaaagcattt gccgcacggc ttaccgcaat cgcgctacgc agattgcctt
     3241 cggcgctatc gctcagccag ataccctgcc cgaggttcag cggttgttca ttgatggctt
     3301 tggtgacgaa agtggcgtca actgcccccg cttctttcag acgcgcagcg ttcagcgcct
     3361 gaagagtcag cagatcgctg gcgacgatat ccagcgtcag catttcgttg tcgagcttca
     3421 gctgctcact ctgcttttcg cccatcagca atgcgcgaag ttcttctgct gttgttgctg
     3481 acttcagttg ttcagcaacg gaatcatcgc tcagtacgtg ggtcagctgg cgtagcaggc
     3541 ccagatgttc atccgagctg gcagcaatac cgatcgccac gtacgctacc tggccgtcac
     3601 cccaggtgac gccttccggg aactgaaata cctgaacgcc ggttttcagc acctgatcgc
     3661 gggtgtcggt agtgccgtgt ggaatagcaa taccattgcc gaggaacgtt gaggtttgct
     3721 gttcgcgcgc cagcatgcca ttgacgtagc cttcagctac attaccggcc tgcaccagcg
     3781 ccgcagcgac ctggcgaatc gcctcttctt tgtctccggc cttttcgccc ggatggatgt
     3841 cctgtacgga taactggaac atagttctcc tctcttgctg aattgaaacg attcagcttc
     3901 tatgagaaaa aaagcgccaa cctggcttag ggttaaagac aagatcgcgc tgaaacgttt
     3961 caagagagca taatactttt gtttcagcac gcaaggaaag ccgtaaagtt atgagcaaaa
     4021 gtttgatgtg ctgcacaatt ttttgtcatt tttcctaatt gctgatggga aaatccgtta
     4081 tcagcaattt catttcagca gcgtcagcag ccaatcagca acagtgaaat ggcattttga
     4141 ttattcgtgg cgcttttgat ttcgctgtct gtttattttc tgacaagcag cgtaaactcc
     4201 gcgtcttcct cttccagtga tcgaccagca tgcataactc ccccgcagtc accagcgcga
     4261 aatcgtttga cctgacctcg acggcgtttt taatcgttgc ctttctcacc ggtattgcgg
     4321 gcgctctgca aaccccgaca ctcagtattt ttcttaccga tgaagtacat gcccgtccgg
     4381 cgatggtggg attcttcttt accggcagcg ctgtcattgg gattctggtg agtcagtttc
     4441 tcgccgggcg ctctgataag cgcggcgatc gcaaatcgct gattgtcttt tgctgcctgt
     4501 taggcgtgct ggcctgcacc ctttttgcct ggaatcgcaa ctactttgtt ttgctattcg
     4561 ttggcgtctt tcttagcagc tttggctcga ccgctaaccc gcaaatgttt gcccttgccc
     4621 gtgaacatgc cgacaaaacc ggacgtgagg cggtgatgtt cagctctttt ttacgcgctc
     4681 aggtttcact ggcatgggtc attggcccac cgctggctta tgccttagcg atgggtttca
     4741 gctttacggt aatgtatctg agcgcagcgg tagcgtttat tgtttgcggc gtgacggtgt
     4801 ggctgttttt accgtcaatg caaaaagaac ttccgctggc gaccggcacg gttgaagcgc
     4861 cgcgccgtaa ccgtcgcgat acgctgctgc tgtttgtcat ttgtacattg atgtggggct
     4921 cgaacagcct gtacatcatc aacatgccgc tatttattat caacgaactg catcttcccg
     4981 agaaactggc cggtgtgatg atggggaccg ccgccgggct ggaaatcccg accatgttga
     5041 ttgccggata tttcgccaaa cgtctgggta agcgtttctt aatgcgcgtt gctgccgtgg
     5101 gtggcgtctg tttttacgca ggaatgctga tggcgcattc acctgtcatt ctgttgggct
     5161 tgcagctgct aaatgctatt tttattggca ttctgggtgg catcgggatg ctctattttc
     5221 aggatttgat gcccggtcag gcaggttcag ccaccacgct ctataccaat acgtcgcgcg
     5281 tgggctggat catcgcagga tcagtggcgg gcatcgtcgc cgagatctgg aattatcacg
     5341 ctgtgttctg gtttgcgatg gtgatgatta tcgccactct gttttgctta ctgcggatta
     5401 aagatgttta aggcgcggtc agcatttcca gatcgataag ccaggtcatt gcctgctgac
     5461 gcgagttacc gcacatttca gcgctggctt gtaagcctgc acagactttc ggccgcagtg
     5521 gcgaggtgaa aattttacag cgttgctgtt catcaagctg aatgcagggc gtattggcgg
     5581 gcttgccatc tggcataccg ggaatagggc tggaaatcga aggggcagtg caacacgccc
     5641 cgcaacccgg acggcattcc atacgttgtt ctcttaaggt taaccaccgg attgatgagc
     5701 gcgcacagta ccaccttttt tgcaccagca aaagtgcgaa taccacttgc ccgaaaggcc
     5761 cgtcgcgagt actttgtcgc gatatttttg acattttcga ctacaggaat ttttcgatgc
     5821 caagagcgaa cgaaattaaa aaaggtatgg tactgaatta caacggcaaa ctgctgctgg
     5881 ttaaggacat tgatattcag tcgcccactg cccgcggtgc cgctacgctg tacaaaatgc
     5941 gtttttctga tgtccgtacc gggctgaaag tagaagagcg tttcaaagga gatgatatcg
     6001 ttgacaccgt gacgctgacc cgccgttacg ttgacttctc ctatgtcgat ggcaacgaat
     6061 atgtctttat ggataaagaa gactataccc cgtatacctt caccaaagat cagattgaag
     6121 aagagttgct gtttatgcca gagggcggca tgccggacat gcaggtgctg acctgggacg
     6181 gtcaactgct ggcgcttgag ttgccgcaga ccgttgatct ggaaatcgtt gaaaccgcac
     6241 caggcatcaa aggggcatct gccagcgccc gtaacaaacc ggcgacattg agcactggtc
     6301 tggtgattca ggtaccagaa tacttaagcc cgggcgaaaa aattcgtatc catatcgaag
     6361 aacgccgtta tatggggcgt gctgactaac ttcagccgca tgcagaaaag ggatagctca
     6421 ggctgtccct tttttaattt attataccaa tcttctattt tgcgcttcct gaacacctta
     6481 cgcccgcaat tttcactcgt tgattgatcg ccctcacact tcatcgcatt aacaatccag
     6541 accaatttca attgctgtca tataacttta cactgtcatt gttaattaat cgttactaag
     6601 acgtgactcc tatgaataca atcgcctccg ttacgctccc gcatcatgta cacgctccac
     6661 gctacgatcg ccagcagttg caatcacgta tcgttcattt tggctttgga gcctttcacc
     6721 gcgcacatca ggcgttactg accgatcgtg tgctgaatgc ccagagcggc gactggggga
     6781 tctgtgaaat cagcttgttc agcggtgatc aactgatgag ccagctccgc gcacagaacc
     6841 atttatacac cgtgctggag aaaggggcgg acggcaatca ggcgataatt gtcggtgccg
     6901 ttcacgaatg cctgaatgca aaactggatt ccttagcggc aattattgag aaattttgcg
     6961 agccacaggt ggcaattgtt tccctgacga ttaccgaaaa aggctattgt attgacccgg
     7021 ccaccggtgc actcgacacc agcaatccgc ggattattca cgatctacaa acccctgaag
     7081 aacctcactc cgcaccgggt attctcgtcg aagcactgaa acgccgccgt gagcgcggcc
     7141 ttacaccgtt taccgtgctc tcctgcgaca atattcccga caatggccat gtggtgaaaa
     7201 acgcggtgct gggaatggca gaaaaacgtt cgccagaact cgccgggtgg ataaaagagc
     7261 acgtcagttt tccgggaact atggtcgacc gcattgttcc ggcagcaacc gacgaatcac
     7321 tggcggaaat cagccagcat ctgggggtaa atgatccctg cgcgattagc tgcgaaccgt
     7381 ttatccagtg ggtggtggaa gataacttcg tcgctgggcg tcctgcctgg gaagtcgcag
     7441 gtgtacaaat ggtgaatgat gtcctgccat gggaagagat gaaactgcgt atgcttaatg
     7501 gcagccactc ttttctcgct tatctgggtt acctctcagg attcgcccat atcagtgatt
     7561 gcatgcagga tcgcgcattt cgccatgccg ccagagcatt aatgctggat gagcaagcgc
     7621 cgacactgcg aattaaagat gtcgatttaa cacaatatgc ggataagtta attgcacgtt
     7681 ttgctaaccc ggcgctgaaa cataagacct ggcaaatcgc aatggatggt agccagaaat
     7741 taccgcaacg tatgctggca ggtattcgca tacatctggc gcgcgaaacg gactggtcgt
     7801 tgctggcatt aggcgttgca ggctggatgc gttacgtcag cggcgttgat gatgccggaa
     7861 ataccattga tgttcgcgat ccgcttagcg ataaaattcg cgatcttgtt gcgggcagca
     7921 gcagtgagca acgcgtcacc gccctgcttt ccctgcgtga agttttcggt gatgatctgc
     7981 cagataaccc gcattttgtg caggccatcg aacaagcctg gcaacaaatc gcacaattcg
     8041 gcgcgcatca ggcgctatta aacaccctca aaatttaacg atttctgcgg ttaaagcgga
     8101 tgaagctcgc cttcgtccgc tctccccttc tcttttctgc cgtttttagc caggattaac
     8161 gctcagttaa cttttaagag tattgcggag ccgttgtgac caggaccaac ctcatcaccg
     8221 gttttctcgg cagcgggaaa accacgtcga ttcttcatct gttagcccat aaagatccca
     8281 acgaaaaatg ggcggtactg gttaatgaat ttggggaagt cggaattgat ggtgctttgc
     8341 tcgccgatag cggcgcattg ctgaaagaga tccccggcgg ctgcatgtgc tgcgttaatg
     8401 gtttacccat gcaggtagga ttgaatacct tactgcgtca gggaaaacca gaccgcttgt
     8461 tgatagagcc gaccggactg ggtcatccga aacagatcct agatctctta accgcgcccg
     8521 tgtatgaacc gtggatagat ctgcgcgcca cgttgtgcat tctcgatcca cgcctgctgc
     8581 tggacgaaaa aagcgccagc aatgaaaact tccgtgacca gctggctgcc gcagacatta
     8641 ttgtcgccaa taaatccgac cgtgcgacgc ccgaaagtga gcaagcgcta cagcgctggt
     8701 ggcagcaaaa tggtggcgat cggcaattaa ttcacagcga gcatgggaaa attgacggtc
     8761 atcttctgga tttgccgcgt cgcaatttag ccgagttgcc cgccagcgcc gcgcattctc
     8821 atcagcatgg cgtgaaaaaa gggttagcag cgttaagcct gccagagcat caacgctggc
     8881 gtcgcagtct gaacagcggg caaggatatc aggcctgtgg ctggatattc gacgctgata
     8941 cggtattcga caccattggc attctggaat gggcgcgact tgcaccggtg gaacgcgtca
     9001 aaggcgtgat gcgtattccc gaagggctgg tgcgaatcaa ccgtcagggc gatgacctgc
     9061 acattgaaac gcaaaacgtt gcgccaccgg acagtcgtat tgagctgatt tccagcagcg
     9121 aagctgactg gaatgccttg cagagcgcgc tgttgaagct tcgtttagcg accaccgcgt
     9181 aaggttgcct gcgtttttca gtaagataat tagagaaaat atgattaaaa atttgccgca
     9241 aatagtgttg ttgaatattg tcggcctcgc gctgtttctt tcctggtata ttcccgttaa
     9301 tcatggattc tggttgccga ttgatgcgga tattttttat ttttttaatc agaaactggt
     9361 cgaaagtaag gcctttttgt ggctggttgc attgaccaac aatcgcgctt tcgacggttg
     9421 ttcactgctg gcgatgggaa tgttgatgct gagtttctgg ctgaaagaaa acgccccagg
     9481 cagacgacgt atcgtgatta ttggtctggt catgctatta actgcagtgg tattaaacca
     9541 gctgggtcag gcattaattc ctgtaaaacg ggccagccca acattgactt ttaccgatat
     9601 taaccgcgtc agcgaactgc tctctgttcc cacgaaagat gcctcacgag atagctttcc
     9661 cggcgatcac ggtatgatgc tgcttatttt ttcggcattc atgtggcgtt atttcggcaa
     9721 agttgcaggc cttatcgccc ttattatttt tgtggttttt gcgtttccca gagtaatgat
     9781 tggcgcacac tggtttactg acatcattgt cggttcgatg accgtgatat tgatcggttt
     9841 gccctgggtg ttgctgacgc cattaagtga tcgattaatc accttttttg acaaatcact
     9901 accgggaaaa aacaaacatt tccaaaacaa ataactcaca gtaattaaca tcatcagggt
     9961 tatttttata gtgaggataa tcctgatgat gcgcaccgtg ctttcatcca tcgcgcgcaa
    10021 aaatcattct ctaagtaaat gaatggattg catgcgtttc actcaatcgt actttaattg
    10081 accaatcccg tttattaact ttctgtatca ctttttctta taaaaaatca tgtaaaaccg
    10141 ctcgccaaga ccgcaccaat cgggtaatct cgaactcgtt ttgcctcggc ggtagattat
    10201 cctcacagca tataattttg tgcgttagtc cacagatttg gccttaagga attgtttcaa
    10261 catgcccagg taattagtct cgtgttgctt ggcatttttt tataacgata tttgtcgtta
    10321 aggacttcaa gggaaaacaa acaacatggt caaatctcaa ccgattttga gatatatctt
    10381 gcgcgggatc cccgcgattg cagtagcggt tctgctttct gcatgtagtg caaataacac
    10441 cgcaaagaat atgcatcctg agacacgtgc agtgggtagt gaaacatcat cactgcaagc
    10501 ttctcaggat gaatttgaaa acctggttcg taatgtcgac gtaaaatcgc gaattatgga
    10561 tcagtatgct gactggaaag gtgtacgcta tcgtctgggt ggcagcacta aaaaaggcat
    10621 cgattgttct ggtttcgtac agcgtacatt ccgtgagcaa tttggcttag aacttccgcg
    10681 ttcgacttac gaacagcagg aaatgggtaa atctgtttcc cgtagtaatt tgcgtacggg
    10741 tgatttagtt ctgttccgtg ccggttcaac aggacgccat gtcggtattt atatcggcaa
    10801 caatcagttt gtccatgctt ccaccagcag tggtgttatt atttccagca tgaatgaacc
    10861 gtactggaag aagcgttaca acgaagcacg ccgggttctc agccgcagct aataaaccgt
    10921 ttggatgcaa tcccttggct atcctgacga gttaactgaa agcactgctt aggcagtgct
    10981 tttttgtttt cattcatcag agaaaatgat gtttccgcgt cttgatccag gctatagtcc
    11041 ggtcattgtt atcttttaaa tgttgtcgta atttcaggaa attaacggaa tcatgttcat
    11101 acgcgctccc aattctggac gtaagctcct gcttacctgc attgttgcag gcgtgatgat
    11161 tgcgatactg gtgagctgcc ttcagttttt agtggcctgg cataagcacg aagtcaaata
    11221 cgacacactg attaccgacg tacaaaagta tctcgatacc tattttaccg acctgaaatc
    11281 cactactgac cggctccagc cgctgacctt agatacctgc cagcaggcta accccgaact
    11341 gactgcccgc gcggcgttta gcatgaatgt ccgtacgttt gtgctggtga aagataaaaa
    11401 aacattctgt tcatctgcga ctggtgagat ggacattcca ctaaaagaat tgattccggc
    11461 gctcgacatt aataaaaatg tcgatatggc gatcttaccc ggtacgccga tggtgccgaa
    11521 caaacccgca atcgtcatct ggtatcgcaa cccgttgttg aaaaatagtg gcgtgtttgc
    11581 cgcgctgaat ctcaatctga cgccttctct cttttatagt tcacggcagg aagattacga
    11641 tggcgtcgcc ctcattattg gtaatactgc gctatctacc ttttcttcac gtttgatgaa
    11701 cgttaatgaa ttaaccgaca tgccggtccg tgaaactaaa attgcgggca ttcctctgac
    11761 cgttcggctt tatgcagatg actggacatg gaacgatgtg tggtacgcat ttttactggg
    11821 tggcatgagt ggaactttcg ttggacttct ctgctattac ctgatgagtg tgcgtatgcg
    11881 cccaggcaga gaaatcatga ccgccatcaa gcgcgaacaa ttttacgtgg tgtatcaacc
    11941 ggtggtggat acacaagctt tgcgggtaac gggcctggaa gtactgctac gctggcggca
    12001 tccagtagca ggagaaatcc ccccggatgc cttcattaac tttgccgaag cgcaaaagat
    12061 gattgtgcca ctgactcagc acctgtttga gttgattgcc cgcgatgccg cagaattaga
    12121 aaaagtactg ccggtaggcg tcaaatttgg cattaacatt gcgccggccc acttgcacag
    12181 cgaaagcttt aaagcggata tccagaaact gctcacttcc ctgcccgcac accatttcca
    12241 gattgtgctg gaaattaccg agcgcgatat gctgaaagag cgagaagcca cacaactctt
    12301 cgcctggctg cattcggtcg gcgtagaaat tgctattgat gacttcggca ccgggcacag
    12361 cgcgcttatc tatcttgagc gttttacgct cgattatctg aaaattgatc gtggatttat
    12421 caacgccatc ggtacggaaa cgatcacttc acccgtactt gacgcggtgc tgacgctggc
    12481 gaaacgcctc aatatgctga cggttgctga aggggtcgaa acgccagaac aggcacgatg
    12541 gctaagcgaa cgcggcgtta atttcatgca aggctactgg attagtcgcc cgttaccgct
    12601 ggacgatttt gttcgctggc tgaagaaacc gtatacgccg cagtggtaag gtgtgcttac
    12661 gtcccttatt attcatagtg aaagcatgcc ggattgcggc taatgatgag taaaaggaaa
    12721 tccgttgcag atgattgtgc gcatactgct gctgtttatc gctctgttca cctttggtgc
    12781 gcaggcgcag actatcaagg aaagctatgc ctttgccgta ctgggcgaac cccggtacgc
    12841 atttaatttc aaccattttg attatgtgaa ccccgccgcg ccaaaaggtg ggcaaataac
    12901 gttgtctgcg ctcggcacct tcgataattt caaccgctat gcactgcgcg gcaatgcggg
    12961 cgcacgcacc gagcagctgt acgacacgct atttacgact tccgatgacg aaccaggcag
    13021 ttattacccg ctgattgctg aaagcgcacg ctatgctgac gattattcct gggtggaggt
    13081 cgctattaat ccacgcgccc gttttcatga tggttcgccc attactgccc gcgatgtaga
    13141 gtttactttt caaaaattta tgaccgaagg cgtgccgcaa tttcgtctgg tctacaaagg
    13201 caccaccgtc aaagccattg cgccgttaac cgtgcgcatt gagttagcga aacccggcaa
    13261 agaagatatg ctgagcctgt tttcgctgcc ggtctttcca gaaaagtact ggaaggatca
    13321 caaacttagc gacccgctcg ccacgcctcc gcttgccagt ggtccgtacc gaattacgtc
    13381 ctggaaaatg gggcaaaata ttgtctattc ccgcgtgaaa gattactggg cagcaaactt
    13441 accggtaaac cgtggacgct ggaatttcga caccattcgc tacgattatt acctcgatga
    13501 taatgtcgcc tttgaagcgt ttaaagccgg tgcctttgat ttgcgtttgg aaaacgacgc
    13561 caaaaactgg gtcacgcgct ataccggtaa aaatttcgat aagaaataca tcatcaaaga
    13621 tgagcaaaag aacgaatcag cccaggatac gcgctggctg gcgtttaata tccaacgtcc
    13681 ggtattcagc gatcgccggg tgcgggaagc aatcactctc gcctttgact ttgaatggat
    13741 gaacaaggcg ttgttttata atgcctggag tcgcacgaac agttattttc agaataccga
    13801 atatgcggcc agaaattacc ccgacgccgc ggagctggtg cttctggcac caatgaaaaa
    13861 agatctaccg ccagaagtct tcacgcaaat ctaccagccg ccggtatcca aaggcgatgg
    13921 ctacgatcgt gacaacctgt taaaagccga caaacttctc aacgaagcgg gctgggtgct
    13981 gaagggtcag caacgcgtaa atgccacaac gggtcagccg ctcagctttg agttattgct
    14041 accttcgagc agcaatagtc agtgggtatt gccgttccag cacagcctgc aacggctggg
    14101 tatcaacatg gacattcgca aagtggataa ctcgcaaatc accaaccgca tgcgcagtcg
    14161 cgactatgac atgatgccgc gcgtatggcg ggcgatgccg tggcccagtt ccgatttaca
    14221 gatttcctgg tcatcggaat atatcaattc cacttataat gcccccggcg tgcaaagccc
    14281 ggttatcgac tcgctgatca accaaattat tgccgcgcag ggaaataaag aaaaattact
    14341 gccgttgggg cgagcactgg atcgcgtatt aacgtggaat tattacatgc tgccaatgtg
    14401 gtacatggcg gaagaccgtc tcgcctggtg ggataaattc tcccaacccg ctgtacgccc
    14461 tgtttacagc ctgggtattg atacctggtg gtatgacgtt aataaagcga cgaaactgcc
    14521 gtcagccaga caacagggag agtagatggg cgcttatctg attcgccgtc tgttgctggt
    14581 gatcccaacg ttatgggcga ttatcactat caactttttc atcgtgcaaa ttgcgcctgg
    14641 cggtccggtc gaccaggcca tcgccgccat tgagtttggt aatactggtg tattacccgg
    14701 tgcaggcggt gaaggtgttc gcgccagcca tgcacaaacg ggcgtcggta atatcagcga
    14761 cagtaattac cgtggcggac gcggattgga tccagaagtg atcgccgaga tcactcatcg
    14821 ctacggtttc gataagccga tccacgaacg ttacttcaaa atgctctggg actacatccg
    14881 ttttgatttt ggcgacagcc tttttcgcag cgcctcggtg ctgacgctga ttaaagacag
    14941 tctgccggtc tccatcaccc tcggattgtg gagcacgctg attatctatc tggtgtcgat
    15001 tccgttaggc attcgcaaag ctgtttataa tgggagccgt tttgacgtct ggagtagtgc
    15061 atttatcatc atcggctacg ccattccggc ctttttgttt gccatcctgc tgattgtctt
    15121 cttcgcgggc ggcagctatt tcgacctgtt ccctctgcgc ggcctggttt ccgctaactt
    15181 tgattcgctg ccgtggtatc agaaaatcac cgattatctg tggcatatca cgctgccggt
    15241 gctggcgaca gtgattggcg gctttgcggc tctgaccatg ctgacaaaaa actcattcct
    15301 tgatgaagtg cgtaagcaat acgtggtgac ggcacgtgcg aaaggggtaa gtgaaaaaaa
    15361 tattctctgg aaacatgtgt tccgcaacgc catgctgctg gtgattgccg gttttccggc
    15421 gacgtttatc agcatgtttt ttaccggctc gctgctgatt gaggtgatgt tttcactcaa
    15481 tggtcttggc ttactgggct acgaagcgac cgtctcgcgc gattatcctg taatgtttgg
    15541 taccttgtat attttcaccc tgattggcct gctgctgaat attgtcagtg atatcagcta
    15601 tacgctggtc gatccgcgta tagattttga gggacgttaa tgtcgcgact cagccccgtc
    15661 aatcaggccc gttgggcgcg ttttcgtcat aaccgtcgcg gctactggtc gttatggatt
    15721 ttcctcgtct tgtttggttt gagtttgtgt tctgaactta tcgccaacga taaaccgttg
    15781 ctggtgcgtt atgacggcag ttggtatttc ccgttgttga aaaactacag cgaaagcgat
    15841 tttggcggcc cgctggcaag tcaggctgat tatcaggacc cgtggctgaa acaacggctg
    15901 gaaaataacg gctgggtact gtgggcaccg attcgctttg gtgctaccag tatcaacttt
    15961 gctaccgata agcccttccc ttctccaccc tcccggcaaa actggctggg aacggatgcc
    16021 aatggcggcg atgtgctggc gcgcattctc tatggcacgc ggatctcggt tctgtttggc
    16081 ctgatgctga ctctctgttc cagcgtgatg ggcgtgctgg cgggggcgct acaaggctat
    16141 tacggcggta aagtcgatct ctgggggcaa cgctttattg aagtatggtc agggatgccg
    16201 acgctgtttt tgattatttt actttccagc gtcgtacagc ctaacttctg gtggctgctg
    16261 gcaattaccg tcttgtttgg ctggatgagt ctggtcggcg tggtgcgggc ggagttttta
    16321 cgtacccgta atttcgacta catccgcgcg gcgcaggcgc ttggcgtcag cgatcgcagt
    16381 atcatcctgc gtcatatgtt gccaaatgcc atggtcgcta ccctcacctt tttaccgttt
    16441 attttatgta gttcgataac caccctgacc tcactcgatt tcctcggctt cggtctgccg
    16501 ctcggttcac cgtcactcgg cgaactgctg ttacaaggga aaaataacct tcaggccccg
    16561 tggcttggga tcaccgcctt cttgtcggtg gcgatattgt tgtctttgct gatctttatt
    16621 ggtgaagccg tccgcgacgc atttgatcct aataaggcgg tgtagcatga cgcaaactct
    16681 gttagcgatt gaaaatttgt cggtgggttt tcgccatcag caaaccgtac gcacagtagt
    16741 caatgatgtt tcacttcaga ttgaggctgg cgaaacgctg gcgctggtgg gtgagtcagg
    16801 ttcaggcaaa agcgttaccg cgctgtcaat tttacgcctg ctcccttccc cgccggttga
    16861 atatctctcc ggcgatattc gttttcatgg cgaatcgctg cttcatgcca gcgatcaaac
    16921 gttgcgcggt gtacgcggta ataagatcgc catgattttt caggagccga tggtgtcgtt
    16981 aaatccatta cataccctgg aaaaacagct ttatgaagta ctttcactcc accgcgggat
    17041 gcgtcgggaa gcggctcgtg gcgaaattct taactgcctt gatcgcgtcg gtatccgcca
    17101 ggcggcaaaa cggctgacag attatccgca tcagctctcc ggcggcgaac gccagcgggt
    17161 gatgattgcg atggcgctgt taacgcgacc ggaattatta attgccgatg aaccaaccac
    17221 cgcgctggac gtctctgtcc aggcgcagat tttacagctg ttgcgcgaac tgcaaggcga
    17281 gctgaatatg ggtatgctgt ttattactca taacctcagc attgttagaa aactggccca
    17341 ccgcgtggcg gtaatgcaaa acggtcgctg tgtcgagcaa aataacgccg ctacgctatt
    17401 tgcctcaccc actcatcctt acacacaaaa gctactcaac agtgaaccgt caggcgaccc
    17461 ggtcccattg ccagaacctg cctcaacgtt gctggatgtt gaacagcttc aggttgcctt
    17521 ccccattcgc aaagggattt tgaagcgcat tgtggatcat aatgtggtgg tgaaaaacat
    17581 cagttttacg ctacgagcgg gtgaaacact gggtttagtg ggcgagtctg gttccgggaa
    17641 aagcacgacg ggactggcgc tgctgcgact gattaactct cagggcagca tcgtctttga
    17701 cggtaagcca ctgcaaaatt taaatcgccg ccagctgtta cctattcgtc atcgcattca
    17761 ggtggtattt caggatccaa actcctcgct caacccacga ctcaacgttt tgcagattat
    17821 tgaggaaggc ttacgggttc accagccgac gctttctgcc gcgcaacgcg aacaacaagt
    17881 gatagccgtg atgcatgaag tgggattaga tcctgaaaca cgccaccgtt atccggcgga
    17941 gttctctggt ggtcagcggc aacgtattgc gattgccagg gcgttaattc ttaagccctc
    18001 gctgatcatt cttgatgaac caacatcatc actcgacaaa acggtgcagg cgcaaatatt
    18061 gacgctattg aaatcattgc aacaaaagca tcaactggcc tatttgttta tcagccacga
    18121 tttgcacgtt gtccgcgcgt tatgtcatca ggttatcgta ctgcgacaag gggaagtagt
    18181 ggaacaagga ccgtgcgcgc gcgtgtttgc cgcaccgcag caggagtata cgcgtcagct
    18241 actggcgttg agctgacgct taaaaaggat tgtagtttga aaagggttcg gcaattgcca
    18301 caccaaaatt tttcaatcgg catgttgcag caaattcgtc ctggcgattt acaaacaggc
    18361 acggctcacc ttcacactcc agcaccgaac atggaacttc gatgtcgctt aacgcctggc
    18421 ttagtttgta catcaccgac catgcattat caccatccgg gctaagcaat ttaagcgcca
    18481 ccaggctgtt gtcaccgcac ggtccatttt gcggaatcgg ttcaaccttc gaacctgcga
    18541 aaccagcaga ccagcgatag ttctgcggca gtcgatggac gattgagagt tgtaatgaag
    18601 tcacttattt tccccggaag tacattttac ttcacaattt gtttacatca attttaacac
    18661 atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct gcgtggcctc
    18721 tatggctctg atttaaataa tatctgcggg cgtggtcacg tttgcggggc tatatgtacc
    18781 cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt acataaattg
    18841 attttacata aaataaacat atatcggggg aatgatagat ttgtggttga catgcatcaa
    18901 caaaggaagc cttttagctt cctcgttgtg caatagatca ccgttttttc ggccgactgg
    18961 cgtacaaaca gaagagaatg gagctggttg cgcagaatgc aattgaccaa atcatcggcc
    19021 atgcagagtt aaaggtcgca agagaaagta atgcgccaac aattgcccct atgccgaaac
    19081 ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca tcaagaatga
    19141 ccgccatcgc attggatgac accatcgaca cgcagcccac aaacgccgca acgccaacca
    19201 ccagcgacca gaatcccagc cccagcagtg cactgatgac catccacgct gccataataa
    19261 actgtatcca caaccccgag cggaacatat ttaacgcgcc aatacggcgg acgaagcggc
    19321 tgttaaagat ggtcatcacg aacagaaaaa gaatgtttag cgcaaagtaa taaccaaagt
    19381 tttccggcgc gacgtggttg atttcaatgt agacaaacgg tccggcgctt aagaatgaga
    19441 acatcccggc aaagctgaaa ccgctggcca gcatataact caggacacgc ttatgacgga
    19501 acagcgccgc aaagttacca atagtggtac gaatgtgaaa aggctggcga cgctccggtg
    19561 gtaaggtttc tttgatcaga aagaagatca ttgccgaagc cagaatcgcc gccacggcga
    19621 ggatccagaa gatgtaatgc cagctcagcc acaccagcac ccagccgcca actatcggtg
    19681 ccatcagcgg tgcaatggtc gtcaccagca tgacaaacga catcatccgt gagaactctt
    19741 ctttcgggta gatatcacgc atcaaagcgt tgataaccac gctggccgca gccgcagcca
    19801 gcccgtggaa gaaacgcatc acaatcagct gatcgatggt ttgtgccaac gcacacgcca
    19861 ccgcggcggc ggcaaacacc agcgtaccgc caagaaccac cggcttacgc ccgaagctgt
    19921 ctgccatcgg cccgtagatt aactgcccca acgcaaagcc cagaatataa gtactgaggg
    19981 tcatctgcgt actgcccgcc ggtacaccaa actgagctga aattaccggt agcgcaggca
    20041 gatacatatc aatcgacagc ggcatcaaca tggccagcag gccaaggata aaaacaatag
    20101 caaacgacga atgctgtcgg gtggtcacaa cgggctcctg aaagtcattg aaaagttaga
    20161 cgacgctggc aatttcttct tcagttaacg gacgatattc accgggagct aaatcagcat
    20221 ccagcgtaat gccgccaata cgttcacgat gcagctcaac cacatggtta cccacggcgg
    20281 cgaacatgcg tttcacctga tgataacgcc cttcgctgat ggtcagacga acctgcgttg
    20341 gggtaatcac ttccagcacc gccggcttag tgagatcttt ttcgttatgc agctgcacgc
    20401 ctttagcaaa ttgctctgcc gtatcgtcag ctacgggtga ttccagcgtc accagatagg
    20461 tcttctcgca atgatggcgc ggggaagtaa tgcggtgcga ccactgacca tcatctgtca
    20521 ttagtaccag accggtggta tcgatatcca gacgccctgc agcgtgcagt ttccacgcta
    20581 ccggttcatc aagaaaatag agcaccgttg ggtgatccgg gtcgtccgtg gagcaaacat
    20641 agccctgagg cttattgagc atgaagtaac gtggaccgtg ttgctgcgcc agcgggttgc
    20701 catcgtaagc gacatcatgt tcagggagca gtttgaacgc tgcattacgg acgatttcgc
    20761 catcgacggt gacacgattg ccgcggattt cacgcccggc aatagcacgg ctaacgccga
    20821 gttgctgtgc gataaattta tcaagtcgca tgtgtgtgat tttgcctgta aaaaacggag
    20881 gtcgggtaat gacccgaaaa tctgaactgt tgtctgccca gtatagcggt ctaacaacat
    20941 ccctcaaggg aaaaagatcc atggcatact attagcagaa taatctacct acgcgagacc
    21001 atgattttta cacttcgccc atatcagcaa gaagccgtgg atgccacgct caaccatttt
    21061 cgtcgtcata aaacccctgc cgttatcgtg ctgcccaccg gtgcaggtaa aagcctggtg
    21121 atagcggaac tggcgcggct ggcgcgtggt cgcgtgctgg tgctggcaca cgttaaagaa
    21181 ctggtggcgc aaaaccatgc aaaatatcag gcgctggggc tggaagccga tatttttgcc
    21241 gccgggctaa agcgcaaaga gagccacggt aaagtggtat ttggcagcgt gcagtcggtc
    21301 gcccgtaatc ttgatgcctt tcagggtgaa ttttcgctgt tgattgtcga tgaatgtcac
    21361 cgtattggtg acgatgaaga gagccagtat cagcaaatcc tcactcacct gaccaaagtg
    21421 aatccccact tacgcctgct ggggctgact gccacgcctt ttcgactggg caaaggctgg
    21481 atttatcagt tccattatca cggcatggta cgcggcgatg agaaagccct tttccgtgac
    21541 tgcatttatg agctaccgct gcgttatatg attaaacacg gctatctgac gccgccagaa
    21601 cgactggata tgccagtagt gcaatacgat ttcagccgct tgcaggcaca gagtaacggg
    21661 ctattcagcg aagccgatct caaccgcgag ctgaaaaaac agcaacgcat taccccgcac
    21721 atcatcagcc agattatgga gtttgctgta acgcgcaaag gggtgatgat tttcgccgcc
    21781 accgttgaac acgcaaaaga gattgtggga ctgctgcctg ccgaagatgc tgcactgatt
    21841 actggcgaca cccccggcgc tgagcgcgat gtgttaattg aagattttaa agcccagcgt
    21901 tttcgctatc tggtcaacgt cgcggtactg accaccggat ttgacgcccc gcacgtcgat
    21961 cttatcgcca ttctgcgccc taccgaatca gtgagtcttt accaacaaat tgtcgggcgc
    22021 ggtctgcgtc tcgctcccgg caagactgat tgcttaattc ttgattatgc gggtaaccct
    22081 cacgatctct acgcgccgga agttggtaca ccgaaaggca aaagtgacaa cgttccggta
    22141 caggttttct gccctgcctg cggttttgcc aacacctttt gggggaaaac gaccgccgac
    22201 gggacattga ttgaacactt tggtcgtcgc tgtcagggat ggtttgaaga tgacgacgga
    22261 catcgcgaac agtgtgactt ccgtttccgt tttaaaaatt gcccgcaatg taacgctgaa
    22321 aatgatattg ccgcccgccg ctgccgcgaa tgtgacacgg tactggttga tccggatgat
    22381 atgttaaaag cggcgctacg actgaaagac gcgctggtat tacgctgtag cggcatgtct
    22441 ttgcagcatg ggcacgacga gaaaggcgaa tggttgaaaa tcacctatta cgatgaagac
    22501 ggcgcggatg tgagtgagcg tttccgtctg caaacgcccg cccagcgtac cgccttcgaa
    22561 caacttttta tccgtccgca tacgcgcaca ccgggcatcc ctctgcgctg gatcaccgcc
    22621 gccgatatcc tcgcccagca agcattactg cgacacccgg attttgtcgt cgcccgcatg
    22681 aaaggtcagt actggcaagt gcgtgaaaaa gtgttcgatt acgaaggtcg ttttcgtcgg
    22741 gcgcacgaat tacgcggtta atggtagttt tgattgatgt ataaggcgat tgagtataga
    22801 atctcgcccg cttttgcata cgcaaagcag atcacttacc tgttgctggg tcgcctgtag
    22861 caggattaat ttaagagaga aagaaatgtt tactatcaac gcagaagtac gtaaagagca
    22921 gggtaagggt gcgagccgcc gcctgcgtgc cgctaacaag ttcccggcaa tcatctacgg
    22981 tggcaaagaa gcgccgctgg ctgtcgaact ggatcacgac aaagtcatga acatgcaagt
    23041 taaagctgaa ttctacagcg aagttctgac catcgttgtt gacggtaaag aaatcaaagt
    23101 taaagctcag gacgtacagc gtcacccgta caaaccgaag ctgctgcaca tcgacttcgt
    23161 tcgcgcttaa ttgctgaata agttgtaaaa aaccccgctc cggcggggtt ttttgtctct
    23221 gcaaattatg cctgattacg gtattgctat tttttgcagg ccagataagg cgttcacgcc
    23281 gcatctggca ttcagaacac aaaaccgatt aattgccgcc agacgtccgg cgctgcaatt
    23341 gatcgcgcaa gttcggcggc gtgcctttaa tggtcagggt atcggttgct ggatcccaga
    23401 agatgcgttc acctaacagc atcgcatcaa agttaatagt caagccaccg ccgctaccgg
    23461 caaacttcgt caactgacgc agtgtgctac ggtctgccgg gaagctctct tccagttcgt
    23521 agcctttttc agccgcaaac tccgtgaagc tcacttcgct gacgcctgcc agctctttcg
    23581 acagcgattc cagttcaatc tcttcacctg cctgcagctg ctcattacag tagctataaa
    23641 cctgctggcg cacgttctgt cgctctgctt tatccagttg tgcttcggca gtgaaatcat
    23701 cgaccgcttg caacagccca cggttttgcg ctttcgcgtt taacccttcg ctggcaccga
    23761 ggaaatccat aaagaaatcc gccactttgc gccctactcg ccctttcagg aaagtgagat
    23821 agcgggtcga ctctggattg gtttcccact cggttaaatc gatacgcgca acaatatccg
    23881 catgattgat atcaagataa tgggttgggt tgatgtcgag attttcgtta acacgcatac
    23941 tgctcaggtt gctcagaact gccaccagca gatactccac cgccagatag cgatagtggc
    24001 aaaacagcac gaatccaccg tcagcaaaag gatatttcgc taattcgtca cgcaggcgac
    24061 cggttgccgc gcggctaaat gccagaaagt cctcttcgcc ctgacgctgt aagcgcagcg
    24121 tctgcgccag ttcactctct tcgctaaaca agccgtaagc tttatttttg gcgctataga
    24181 cccgatgcag ttctgccacc atctcaacga cggtttccgt cggttccagc aatgaatcgc
    24241 gcaacaccag ctcaaggttc tgctcatcac gcttgataag ctggtgcaga gcaatctggt
    24301 tgatatccag actcatgata aactctcctt taagaccggg cggtattcaa ccaccgccgg
    24361 ttaagcgacg caataaaaga tggcggtcat tttacagaag gcggcgtaac gagaatgatt
    24421 taacgcataa aaaagcagaa aaaaaaccgt tgctacggta atatgttgcc ctttcatcaa
    24481 ctaactgatt tcgatttatg ccacaaattt cccgctacag tgatgaacag gttgaacaat
    24541 tgctcgcaga gctgctcaac gtactggaaa aacataaggc tccgaccgac ctttccctga
    24601 tggtgttagg caacatggtc actaacctta tcaacaccag cattgccccg gcccaacgcc
    24661 aggcaattgc caactctttt gcccgcgcct tacagtcctc tatcaacgaa gacaaagcgc
    24721 actaagggaa acagataaca ggttatggta actcatcgtc agcgctaccg tgaaaaagtc
    24781 tcccagatgg tcagttgggg gcactggttt gcactgttca atattctgct ttcgctcgtc
    24841 attggcagcc gttacctgtt tatcgccgac tggccgacaa cgcttgctgg tcgcatttat
    24901 tcctacgtaa gcattatcgg ccatttcagc ttcctggtgt tcgccaccta cttgctgatc
    24961 ctcttcccgc tgacctttat cgtcggctcc cagaggctga tgaggttttt gtccgtcatt
    25021 ctggcaacgg cgggaatgac gctattactg atcgatagcg aagtctttac tcgtttccat
    25081 ctccatctta atcccatcgt ctggcaactg gttatcaacc cagacgaaaa tgagatggcg
    25141 cgcgactggc agctgatgtt catcagcgtg ccggttattt tattgcttga actggtgttt
    25201 gcgacgtgga gctggcaaaa gctacgcagc ctgacgcgtc gtcggcgctt cgcgcgcccg
    25261 ctggccgcat tcttatttat cgcctttatc gcctttatcg cctcgcatgt ggtttatatc
    25321 tgggccgatg ccaacttcta tcgcccgatc accatgcaac gcgctaacct gccgctttcg
    25381 tacccgatga cggcgcgacg ttttcttgag aagcatggtc tgcttgatgc gcaggagtat
    25441 caacgccgtc tcattgagca aggtaatcca gacgccgttt ccgttcagta tccgttaagc
    25501 gaactgcgct atcgcgatat gggcaccggg cagaatgtgc tgttgattac tgtcgatggc
    25561 ctgaactact cacgcttcga gaagcagatg cctgcgctgg caggttttgc tgagcaaaat
    25621 atttcgttca cgcgccatat gagctccggc aacactacag acaacggcat ctttggcctg
    25681 ttctatggca tctcgccgag ctatatggat ggcattctgt cgacccgtac gcctgcggca
    25741 ttaattactg cgcttaatca gcaaggctat cagctggggt tattctcgtc agatggcttt
    25801 accagcccgc tgtatcggca ggcattgttg tcagatttct cgatgccgag tgtacgcacc
    25861 caatccgacg aacagaccgc cacgcagtgg atcaactggc tgggccgcta cgcacaagaa
    25921 gataaccgct ggttctcgtg ggtctctttc aatggcacaa acattgacga cagcaatcag
    25981 caggcatttg cacggaaata tagccgggcg gcaggcaatg tcgatgacca gatcaaccgc
    26041 gtgctcaatg cactgcgtga ttctggcaaa ctggacaata cggtggtgat tatcactgcc
    26101 gggcggggta ttccgctgag cgaagaggaa gaaacctttg actggtccca cggtcatctg
    26161 caggtgccat tagtgattca ctggccaggc acgccggcgc agcgtattaa tgcgctgact
    26221 gatcataccg atctgatgac gacgctgatg caacgcctgc tacatgtcag cacacctgcc
    26281 agcgaatatt cgcaaggtca ggatttgttc aaccctcaac gccgccatta ctgggttacc
    26341 gccgcggata acgatacgct ggcaattacc accccgaaaa agacactggt gctgaacaat
    26401 aacggtaaat accgcactta caacttacgt ggtgaaagag tgaaagatga aaaaccacag
    26461 ttaagtttgt tattgcaagt actgacagac gagaagcgtt ttatcgctaa ctgattaatt
    26521 ataaatcagt tagcaaaata tcttacttgc aatcggtgtg gaaaacggta gtattagcag
    26581 ccacgagtcg gcacgtagcg cagcctggta gcgcaccgtc atggggtgtc gggggtcgga
    26641 ggttcaaatc ctctcgtgcc gaccaaaaat cccaagaaaa ccaacctatt gcggttggtt
    26701 tttttatatc tgcatttaat tcgataaaca gacagcgaca catcacgacc tgtttatttt
    26761 ctgttatcag aacgtccaga ctacacccgc ctgagttgtg ctgctttctt cacgggagaa
    26821 acgactatcg ataccgagcg tcaggcccgt caatgtttgc tcaaagccag ctcccgcatc
    26881 agtgttcacg ttgttgcggc tgttaattgc cgaactccac atcgccgccc tttacgctac
    26941 caagacgctc acgcacggtg tccagttctg catcaaatac cagcggttgt gctgccgcca
    27001 tattcagcac atccgtggtt gaagggggca tttgtacgcg attctctgtg agactccagc
    27061 tatggttgcc attatccagc aaggtatatt catacgtact gatatcaaca acgcttccgg
    27121 cattgcccaa cgtaaatgcc gcatcaccgc gccctccttt aatcgtggga tcagtcgccg
    27181 cgccataaac ctgtagcact ccattattga tcgtagtctg gtttaccgcg ccacccattt
    27241 gaacaatttg ataaccatca tttaatactg agtattctgc ggtgccattt atttcaatgt
    27301 actgatatcc cccgttaatt ttagtattac tacttacacc aaattcttat atatgctgtt
    27361 ctccgccagg attgattttc gtattattgg cggccccata aaccagctgg atatcttttt
    27421 ccaggacaac accatcaact gtctcaccat actcaacagt tgatgccaat gctatgggtg
    27481 aatataacgc tgtagccacc agaacagata ttgcggaacg acaaagagaa acagaaccag
    27541 attggtgcat taagctttca tcctatgaaa ttaattgctg ttaaaagcat tggggacaga
    27601 aaatacccat agctccatac ccggagtcag ttttaaaaaa ctgtttaaag aaatgcacaa
    27661 gtattgtgat tgatttttta gttgtttttc ctgatgagaa gctgatgcaa aattccgtct
    27721 ttataatgaa gatgatgcca aagcgaacga caaagttgta gttttcacta catgtccata
    27781 cataaaatgg ggtaacattc acgcgcctgg tagcgttacc aacgctacgc tcaaacataa
    27841 tgattctaat aaaacctcag gagactacta tgcctgaagc aacgcctttt caggtgatga
    27901 ttgtggatga tcatccactt atgcgacgcg gtgttcgtca gttactggag cttgatcctg
    27961 gctttgaagt ggtcgccgaa gcgggcgacg gcgcgagcgc gatcgatctg gcgaatagac
    28021 tggatatcga cgtgatcttg ctggatctca atatgaaagg tatgagtggc ctggataccc
    28081 tcaatgcctt gcgcagggat ggcgttaccg cgcaaattat tatcctgacc gtatccgatg
    28141 cctccagcga tgtctttgcg ctgatagacg caggcgcaga cggttatctg ttgaaagaca
    28201 gcgacccgga agtgttgctt gaagcgattc gtgccggagc gaaaggcagc aaagttttta
    28261 gcgaacgcgt caatcagtac ttacgtgaac gtgaaatgtt tggcgcggaa gaagatccct
    28321 tcagcgtgct gacggagcgc gagctggatg ttctgcacga gctggcacag gggctgtcaa
    28381 ataaacagat tgcctcggtg ttgaatattt ccgagcagac agtaaaagta cacattcgca
    28441 atctgctgcg taaactcaat gtccgctcac gcgtggcggc caccattctg ttcctgcaac
    28501 aacgcggggc acaataaaaa tagcccgatg gcatcaccat cgggccactt tttatttact
    28561 ctcctgcggc gacaaatgtt gcatcgcctg cgcgatacta cgttcgatca ccgcacggcg
    28621 agtatcgttg gcaggtaaga gtttcaacat catttcccac gcggcaacgg cttcgccaaa
    28681 tcgttgctgc tcaaaggcat taaatgcata catgcttaac acacggatat tgctatggtc
    28741 cgttctcacc aactggcgca gcagttcgcc gccgaggcgg ttgtcgttgg gatcagatga
    28801 acgtgtcaac gcttcagcgt atccgagcgc cgcatcactg ttcttcggat cgaggcgata
    28861 tgcagtagcg tatgcatcgg tggcgatact ggcgttaccc agcgccatgc caacgcggcc
    28921 caacataatc cagccttcta tatctcccgg atttttttgc agttgagtac gcatccccag
    28981 cgcaaggcgc gacatctctt cttcgttgag cggagcggct ttcggatcca gcgccctgtc
    29041 cagtaacgcc ggagcctgcg cggtggcctg ctgccagatt ttcacctgct gataattgcc
    29101 agtctggtag tagctgacgc cagccacaat taacgccacc acaatacccg gcagataaac
    29161 aacatatccg gcacgcttac cttccggcac gctttgttct ggaaacgctt ccggcaccac
    29221 gcgtacccga cgccgcgaac gggcgtatat gacccaaccg ccaatgccaa tagccactac
    29281 tggcagcacc cacagcagca cggtcagcgg cgttaacggc ggatcgtaag tgacgaagtt
    29341 gccgtaacgc gccaccatat aatcgacaat ctctttctta cttttgcctt cctgcatcag
    29401 ttcatacact ttctggcgca ggtcggtggc aatcatcgag ttggaatcgg caatgctgtt
    29461 gttctggcat ttcgggcagc gcagttcttc agtgagctga cggaactgtt gttcctgtgc
    29521 ttcatcttta aactgcaaca cgtcgatggt cgccagcgct gagccggaga tcatcagcat
    29581 cagcacgccc aataaaaacc tcattgtgcg gcctccttac tgtatttctc ccacagtggc
    29641 ttgatctctt cttcccagac gcgaggattg agatcgcccg catggcgata gcgaatgatg
    29701 ccgttgccgt caataaggaa cgtttctggc gcgccataga caccgagatc cagccctaac
    29761 atgccgtcgc catcaaacag gcttagtgcg taaggattgc ccaactcttt cagccagctg
    29821 attgccttct ggcgatcgtc tttatagttc atgccgacca cgcggatgcc ctgcgcagaa
    29881 agctgattca gatattgatg ttccgcacgg caggtcggac accaggtcgc ccagacgtta
    29941 agcagcaccg gtttgccctg agtcagcaca tccgcctgat aaaactgccc cggattgtcc
    30001 agtgattcga ggcgaaactt cggcactagc ttgccaatga gcgccgattc cagattggtc
    30061 ggatcatccc cttcggcatt acgcgccagc tgccacagta gcgccgcggc aatcgccagg
    30121 aagataatca acggaattaa caatactttg cgcttcatac agcctccggc gcagttttct
    30181 gcgggttcac gcgcttacga tagcgaggat caaacaggca ca
//
LOCUS       DAZUCC010000037        30156 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZUCC010000037 DAZUCC010000000
VERSION     DAZUCC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.037"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHY09_001488"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHY09_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS3884476.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHY09_001489"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHY09_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS3884477.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHY09_001490"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHY09_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS3884478.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHY09_001491"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHY09_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS3884479.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHY09_001492"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHY09_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS3884480.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHY09_001493"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHY09_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS3884481.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHY09_001494"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHY09_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS3884482.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHY09_001495"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHY09_001495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS3884483.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHY09_001496"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHY09_001496"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS3884484.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12916..14049)
                     /gene="thiH"
                     /locus_tag="ACHY09_001497"
     CDS             complement(12916..14049)
                     /gene="thiH"
                     /locus_tag="ACHY09_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFS3884485.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINSKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQVKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSISFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14046..14816)
                     /gene="thiG"
                     /locus_tag="ACHY09_001498"
     CDS             complement(14046..14816)
                     /gene="thiG"
                     /locus_tag="ACHY09_001498"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFS3884486.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRACGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIITQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14818..15018)
                     /gene="thiS"
                     /locus_tag="ACHY09_001499"
     CDS             complement(14818..15018)
                     /gene="thiS"
                     /locus_tag="ACHY09_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFS3884487.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQSGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15002..15757)
                     /gene="thiF"
                     /locus_tag="ACHY09_001500"
     CDS             complement(15002..15757)
                     /gene="thiF"
                     /locus_tag="ACHY09_001500"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFS3884488.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRNLALRRASGCPVCGGSNADPV"
     gene            complement(15750..16385)
                     /gene="thiE"
                     /locus_tag="ACHY09_001501"
     CDS             complement(15750..16385)
                     /gene="thiE"
                     /locus_tag="ACHY09_001501"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFS3884489.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16385..18280)
                     /gene="thiC"
                     /locus_tag="ACHY09_001502"
     CDS             complement(16385..18280)
                     /gene="thiC"
                     /locus_tag="ACHY09_001502"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFS3884490.1"
                     /translation="MSVTKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGREHIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18361..18461)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18513..18989)
                     /gene="rsd"
                     /locus_tag="ACHY09_001503"
     CDS             complement(18513..18989)
                     /gene="rsd"
                     /locus_tag="ACHY09_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFS3884491.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19084..19857
                     /gene="nudC"
                     /locus_tag="ACHY09_001504"
     CDS             19084..19857
                     /gene="nudC"
                     /locus_tag="ACHY09_001504"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFS3884492.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19897..20961
                     /gene="hemE"
                     /locus_tag="ACHY09_001505"
     CDS             19897..20961
                     /gene="hemE"
                     /locus_tag="ACHY09_001505"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFS3884493.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSAPYHL"
     gene            20971..21642
                     /gene="nfi"
                     /locus_tag="ACHY09_001506"
     CDS             20971..21642
                     /gene="nfi"
                     /locus_tag="ACHY09_001506"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFS3884494.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21685..22275
                     /gene="yjaG"
                     /locus_tag="ACHY09_001507"
     CDS             21685..22275
                     /gene="yjaG"
                     /locus_tag="ACHY09_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFS3884495.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22462..22734
                     /gene="hupA"
                     /locus_tag="ACHY09_001508"
     CDS             22462..22734
                     /gene="hupA"
                     /locus_tag="ACHY09_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFS3884496.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22747..23442
                     /gene="yjaH"
                     /locus_tag="ACHY09_001509"
     CDS             22747..23442
                     /gene="yjaH"
                     /locus_tag="ACHY09_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFS3884497.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23444..23863)
                     /gene="zraP"
                     /locus_tag="ACHY09_001510"
     CDS             complement(23444..23863)
                     /gene="zraP"
                     /locus_tag="ACHY09_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFS3884498.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMDHW"
     gene            24101..25477
                     /gene="zraS"
                     /locus_tag="ACHY09_001511"
     CDS             24101..25477
                     /gene="zraS"
                     /locus_tag="ACHY09_001511"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFS3884499.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGTGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQVGKGATFTLWLPVT
                     ITRKDPQG"
     gene            25474..26799
                     /gene="zraR"
                     /locus_tag="ACHY09_001512"
     CDS             25474..26799
                     /gene="zraR"
                     /locus_tag="ACHY09_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFS3884500.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENTVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26796..28085)
                     /gene="purD"
                     /locus_tag="ACHY09_001513"
     CDS             complement(26796..28085)
                     /gene="purD"
                     /locus_tag="ACHY09_001513"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS3884501.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28097..29686)
                     /gene="purH"
                     /locus_tag="ACHY09_001514"
     CDS             complement(28097..29686)
                     /gene="purH"
                     /locus_tag="ACHY09_001514"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS3884502.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAISNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7055 a         7829 c         8318 g         6954 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggtgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa tacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtagcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 aaccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagagttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgttgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggcgttgttg aagaagttga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga cccctgtaga gctggacttc agccaggttg
     1381 aaaaagctta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgcgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcactg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgagag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgctctgct gaaagaactg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 gggtgcaaac gctgaagctg ctaaagctgc aggtgctgaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggtaactg agtgagtcgc caggagcata gttaactatg
     2821 tgactggtgc gaacgaagga agccaacgcc gccacaagct gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggcttg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgt
     5281 gctgcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tattctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaata gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctatctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cctgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgacaagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgatatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccaga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggttac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcgtct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcatggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttctgg tcagatccgc
     7981 ctgtacgacg gccgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt actaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggtgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggcctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt acttcgaatc
     8881 ctatgtggtt attgaaggcg gtatgaccaa cctggaacgc cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaaa tgggtgcgga
     9001 agcaatccag gccctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gcagttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcctc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggctttg catatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtt attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa cggcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtact catgaaggta tcatgatgac
    10921 tccggttatc gaaggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcaaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga ccgacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gatgtactga tcccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatctggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 tctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcactccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccaaaatg gcgtcagctc aatgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgatgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaagacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggtgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aactcgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaattctt cacggaattt ttaagaaagg cagggcgaaa
    12781 cgaggaagaa gcttttcgcc ctggacaaca ttcctgctga cgggactacc ataaaaaaac
    12841 acaacgcttt caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg
    12901 ttgttacatt ctgtctcatg gcctttgcga ggcgcgtccc agatagctgt cccagtcttt
    12961 ccataccggc tgcaaaccct gagcagttaa cgcggcagca accgcttccg gtctgcgatc
    13021 gtcgtgcggt gagaactgtt ccagctcggg gtgattgtcg gcatagccac ctggctgcgt
    13081 tttcgaaaaa gcgctgacgt tattaattgc cagcggaata acgcgatcgc gaaaccacgg
    13141 tgattcccgc gtggagagtg acagttcaat ctccggtgca agcagccgga aggcgcagat
    13201 ggtttgcact aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacatgg
    13261 acgcaggcgt gggaaggaga tagagtagcg gctttgccag taatgctgtt gcagccatag
    13321 caaatgttct gcaaccatat agcagtcaac gcgccagctg tcggaaaggc caattagcgc
    13381 gccgaggcct atcttatcaa tccccgcacg gcccagccga tccggcgttt ccagccgcca
    13441 gaagaagtcc tgttttttac ctttcagatg atggcgggca taagtcgcct cgtgatatgt
    13501 ctcctgataa accatcacgc catccagacc tagttgcttt aactcggcgt attccgtctc
    13561 cgccagcggt tgcacttcca tctgtagtga agagaactgt tcacgcaggg cagggagatg
    13621 acgacgaaag taatccatcc ccactttcac ctgatgttca ccagtgacta atagcagatg
    13681 ttcaaagccc atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt
    13741 tttgcgcttg atgcgattgc tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag
    13801 attggaaaga taaagcggga cgtagaagct gacgacgttg ccaaatcgct gacgggtcag
    13861 acgctgcgcc cgttgggcca gtggttccag atagccactg gcggctggcg ataacagcgc
    13921 catcatatcg tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt cagcagccgt
    13981 tttgctgttg atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc gatcgctgaa
    14041 ggttttcatg ctgatgcctc cagaaatccg gtcagcgggc tggtggcatg agcaaaatga
    14101 ctgcggctgc ccggtccgga ctgacgtgcc agcaggcctg cttctactgc cagacgaaat
    14161 gccttcgcca tgttgacggg atcgtccgcg acggcaatcg ccgtattcac taacaccgcg
    14221 tcggccccca tttccagcgc ctgcgcggca tggctgggaa cgccgatgcc agcatcgaca
    14281 accaccggaa ccgtggcttg ctgggtaata atctccagca tggcacgggt ttccagtccc
    14341 tgattcgagc caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga
    14401 cgtttacaca ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc
    14461 agcgtttcgg cggctttcag ggtttcgatg ggatcgggca acagccagcg ggcgtcaggg
    14521 tgaatctcta attttaacca gtttgtgcct aacgcttcac gagccagatg ggcggcgaaa
    14581 atggcttctt ccgctgtttt cgccccggat gtatttggca gcagggtcac acccgccgcg
    14641 ataagcggtt cgaggatagc gtcgttgtgc tggcgcaagt tgacgcgttt catcgccagt
    14701 gtcaccagtt ggctgccgca agcgcggatc gcctccatca tcagttgtga agaggcgaat
    14761 ttccctgtgc cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca
    14821 accccctgca ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgc
    14881 ccactgctca cgcgggacga tttgctgatt aatcgccaga gccgcgcccg attgtcgttg
    14941 gtcgagttgc tccagtagtt cgtgaaccgt ttgcccggcg gcgcactgca tcggttgatc
    15001 gttaaacagg atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg
    15061 ccaggttgcg ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggcgtct
    15121 ctataccgct taataactta atggcttcca gtgcctgtaa agtgcccata accccgacca
    15181 ccgggccaac cacgcccgcc gtgcggcagt tgcgttcagg ctcctggtta tctggccaca
    15241 ggcagcggta acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc
    15301 cgaccgcgct ggcggtgata agcggcgtgt tgagtgccac gcaggcggca ttaatctcct
    15361 gacgagtcgc catattgtcg gtacagtcga gcaccacatc ggcccgtgct accgcatctt
    15421 ttaacgcctc acccgttaac cgttgttgta atgctgtcag ttgaatatcg ggattcaact
    15481 gtgtcagtcg ctgatggctg acctgcgatt tcgggcgatc gatatcttca gtggtaaaga
    15541 ggatttgtcg ttgcagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc
    15601 cgacgccagc gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa
    15661 tcagcacctg gctgtcgagc agtttttgct gcccgtcaag agcgatatcg tcgagcagga
    15721 tttggcggct ataacgcata aagtcacggt cattcatcgc caactcctgc aatttccagc
    15781 aactgtgccg ttgccaaacg ccagtctgcg gcttgagtaa tggcgcttac gacggcgatg
    15841 ctgccgacac ccgttgctat caccgcaggc gcgcgcgcca gactgatacc gccaatcgcc
    15901 acggtgggat aatccgccag gcgctcaaca tgccgtgcca gctgttccag cccctgcggt
    15961 gcagaaggca tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg
    16021 cgtgctgcca gcgcgacgtc gatctccata tcgtcatggg tcgaaacgcc cagtcgcaga
    16081 cctgccgcgc ggatggcgct gagatcggtg gcttgcaaat cttcctgccc cagatggacg
    16141 ccatatgcct gatgcttgat cgccagccgc cagtaatcgt tgataaacaa tcgcgcgtta
    16201 tagcggcggc ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgtcga
    16261 tctttgatgc gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt
    16321 acgctgtcca ccaccgggta cagtcctaaa cgaaagggta caggaggaaa atctggctga
    16381 tacatcacgc ttcctcctta cgcaggtaga tttctccgcc tctggcgcgg aagttctccg
    16441 acatatcagc cattcccact tcaatggttt gcgcggcggc gtaatcgcgc acttcctggc
    16501 tgattttcat cgagcagaat ttcggcccac acatggagca aaaatgggcg actttgccgg
    16561 attcttgcgg cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat
    16621 taaactggtc ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt
    16681 gcgcgcccgg atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa
    16741 gcccctgctt aacatcttct ttattaggca gacccagatg ctcttttggc gttacgtaac
    16801 agagcatcgc gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt
    16861 cataacccgg cgcgatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc
    16921 agtgctctaa ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc
    16981 cttcaatcat cacctgtacg tcatattccc aggcgatttt ggtcagttca cccagcgtat
    17041 gcagctcggc aaattgcgct tcatcgttgg cgtcctgaat agaaccaggg cgcagaccgt
    17101 ctcccagcga cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt
    17161 gatagaggaa attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc
    17221 gagagacgat accggtcagg cgtttcgcgg tcatcggcac atagcgcagc agtacgcccg
    17281 catggatagt gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg
    17341 cttcccaggt aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca
    17401 ctgtaccgat cggcaccggg ctgttacgca aaatccactc gcgggtttcg tgaatatagc
    17461 gaccggtgga gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt
    17521 ccacttcttc ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta
    17581 ccaggaaatt gcgaccaata atcatcggct ccgattccgg atgattaatg ttggccggga
    17641 taatcgcgcg tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca
    17701 gacgtgcgcc aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgtgct
    17761 cgcggcccat attctcgcgg atggcgatga attccatttc cggtgtgatg atgccctggc
    17821 gggcgtagtg cagttgggtg acgcggcgtc ctgctttggc gcgttttggt gttagtacgc
    17881 cgctaaaacg cagctcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac
    17941 tgcgcacggt aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg
    18001 ccagtccttg ctgcacgtta atggcgatct gtggatcgcc atacggaccg gaggtgtcgt
    18061 agaccggaat cgcttcgttt tcttcgtact gcggttgttc tttgctgccg ccaattagcg
    18121 tcgggctaag ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg ccagtgatgt
    18181 aaatgcgttt tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggcct
    18241 gggcgcgttg ttcgcggcgg gtcagttttg ttacagacat agctcattcc aaaaagttaa
    18301 ggacgtggct tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat
    18361 agcagattaa ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga
    18421 ataaacggtc tcagccagtt aaggcactcc gacaagaaat tagccccaca aacggggcgt
    18481 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18541 tcaagtacca gcagaatcaa cttatcttcc agcacaaagc gtgcttccag cgcctcgccg
    18601 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    18661 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    18721 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    18781 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    18841 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    18901 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgt
    18961 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19021 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19081 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19141 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19201 ggtcagcgcg cactacaaat cggcgaatgg caaggggaac ctgtttggtt agtacaacag
    19261 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt agggctgttt
    19321 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19381 tactgcgggc atgaaatgta tccaagcaaa accgaatggg cgatgctgtg cagccattgc
    19441 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19501 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19561 ttcgtcgaag tgggtgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19621 ggaattaaag ttaaaaactt gcgttacgtg acctcccaac cgtggccgtt tcctcagtcg
    19681 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgttatcga cccgaaagaa
    19741 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    19801 gtagcgcgcc gtctgataga agatacggtg gcgatgtgtc gggcagagta tgagtgatga
    19861 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    19921 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    19981 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20041 ctgtgcaaaa atgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctatccg
    20101 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cagacgcgat gggtttaggg
    20161 ctctattttg aagccggaga aggtccgcgt tttacctcac cagtcacctg caaggccgac
    20221 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20281 cgtaccattc gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20341 tggacgctgg cgacctatat ggtggaaggc ggcagcagca aagcgttcac cgtgatcaaa
    20401 aaaatgatgt atgccgatcc acaggcgctg cacgcgctac tcgataaact ggcgaaaagc
    20461 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggctgtgat gattttcgac
    20521 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20581 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtaccggt cacgctgttt
    20641 accaaaggcg gcggacagtg gctggaagcc atggcagaaa ccggttgcga tgcgctgggc
    20701 ctcgactgga caacggacat cgccgatgca cgccgccgcg tgggcaataa agtcgcgttg
    20761 cagggcaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    20821 gcgactatac ttgcaggttt cggtcacggt gaaggtcatg tctttaacct tggtcacggc
    20881 attcatcagg atgtgccgcc agaacatgct ggcgtgtttg tggaggcagt gcatcgcctg
    20941 tctgcgcctt atcatctgta aggagtggtt atggatctcg cgtcattacg cgctcaacaa
    21001 atcgaactgg cttcttctgt gatccgcgag gatcgactcg ataaagatcc accggatctg
    21061 atcgccggag ccgatgtcgg atttgagcag ggcggagaag tgacgcgagc ggcgatggtg
    21121 ctgctgaaat acccctcgct tgagctggtg gagtataaag tggcccgcat cgccaccacc
    21181 atgccttaca ttcctggttt tctctccttt cgtgaatatc ctgcgctgct ggcagcgtgg
    21241 gagatgctgt cacaaaagcc ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct
    21301 cgccgtcttg gcgtcgccag ccattttggt ttgctggtgg atgtgccgac cattggcgta
    21361 gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc
    21421 ccgctgatgg ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac
    21481 ccattgttta tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa
    21541 cgctgcatga aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg
    21601 gaacgtccgg cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat
    21661 aatttccgta tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc
    21721 ctggaaagct ggcagcacgt cacttttatg gcctgcttat gcgaacgtat gtaccccaat
    21781 tacgccgtat tctgtcagca aaccggcttc ggcgatgggc agatttaccg ccgcattctc
    21841 gatctcattt gggaaacgct gaccgtcaaa gacgcaaaag taaatttcga cagccaactg
    21901 gagaaatttg aagaagcgat tccttcagcc gacgatttcg atctgtacgg cgtttatccg
    21961 gcaatcgatg cttgcgtggc gttaagtgaa ctggtccatt cgcgtttaag tggcgaaacg
    22021 ctcgaacacg cggtggaagt gagtaagact tccatcacga ccgtggcgat gctggaaatg
    22081 actcaggctg gtcgcgaaat gagcgatgaa gagctcaaag aaaacccagc tgtagagcaa
    22141 gaatgggaca ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc
    22201 gagctgataa aaggccttag ggcagacctg cgtgaggcgg gtgagagcaa tattggtata
    22261 atttttcagc aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc
    22321 ttccctttcg ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc
    22381 gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata
    22441 aacacattgt aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa
    22501 gcagaactgt ccaaaaccca ggctaaagct gctctggagt ccactctggc tgcaattact
    22561 gagtctctga aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac
    22621 caccgcgctg agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca
    22681 gctaacgtac cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc
    22741 gtggcagtga acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga
    22801 tcattgatgc tggctggcgc tctgtttctc actgcttgta gtcacaactc ttcacttcct
    22861 ccctttaccg ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac
    22921 agcggcgata atgtgcatct tcttgccgtg tttagcccgt ggcgcagtgg cgatacgacg
    22981 acgcgagagt atcgctggca gggcgataac ctcacgctca tcaatatcaa tgtttacagc
    23041 aaacccccgg tgaatattcg cgcgcgtttt gacgatcgcg gcgatctgag ctttatgcaa
    23101 cgcgaatccg atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat
    23161 cgtgctgatc agatccgcca gattagcgat gccttacgtc aggggagagt cgtgctgcgc
    23221 caggggcgct ggcatgcgat ggagcagact gtgaccacct gcgaagggca aaccattaaa
    23281 cctgatttag attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt
    23341 gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc aattactgtt ggtggcaaac
    23401 tctgatttct gtcgctggca acccaacgag aaaacgttct gatttaccag tggtccatac
    23461 ccatatgacc gccaccaccg cagccgccgt aacccattcc ggtaccgcgc ggaatacccg
    23521 cttcagccat cgcaatatct cgtttcaccc gtaactcatc taacgactga cgcaaattct
    23581 ccatctcttt ggcgaccgcg ttaattttgc tgctatccgg tgggttcgcg gctaacaggg
    23641 cattgtattc ataacgcttt gttaccagtt gctgttgcag tgcgctgctt tgagcgtaaa
    23701 agtcattatg gattttctgc cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt
    23761 gctgccacat accgtgtccg ccgtgagcaa atgccgatgt cgatcccatc gccattgctg
    23821 aaagcgccat cattaccagg gcaattttcg tgttccgttt catggttaat cctccagtgg
    23881 ttgtcttact tcgggtattg catcttccgt gccaacgatg aaacgctgat atgacgggta
    23941 atctggcatg ataaacgagt aaaaatgact cgcctgctgc gggtagcgag tcatttttac
    24001 tcattgaaac ttaagccttt gtgttacagc gcagggtaag cgctgataaa agatggcatg
    24061 atttctgctg tcagaaaggg atgagcaggc aaagaagaag atgcgtttta tgcaacgttc
    24121 taaagactcc ttagctaaat ggttaagcgc gatcctcccc gtggtcattg ttgggctggt
    24181 ggggctgttt gcggtgacag tgattcgcga ttacgggcgc gagactgcag ccgccagaca
    24241 aacgctgctg gaaaaaggca gtgtacttat ccgtgctctc gaatccggct cgcgcgtcgg
    24301 catggggatg cgcatgcatc atgcgcagca gcaggcatta ctggaagaaa tggccgggca
    24361 gcctggtgta cgttggtttg cggtcacgga tgaacaagga acaatcgtga tgcatagcaa
    24421 ctccggcatg gtgggaaaac agctttattc cccgcaggaa atgcagcagt tacatccggg
    24481 agatgaagaa gcgtggcggc ggatcgatag cgcagacggc gagcctgttc tggaaattta
    24541 tcgccagttt caaccgatgt ttgctgctgg aatgcaccgg atgcgccata tgcagcagta
    24601 tgccgcgaca ccacaagcaa tttttatcgc tttcgacgcc agtaacattg tgagtgccga
    24661 agatcgtgag cagagaaaca ccctgattat cctcttcgcc ctggcgacgg tcttgctggc
    24721 aagcgtgttg tcattcttct ggtatcgccg atatctgcgc tcgggccagc tgttgcagga
    24781 tgaaatgaag cgcaaagaga agctggtggc actggggcat cttgcggcag gcgttgccca
    24841 cgaaatccgt aacccacttt cctcgattaa agggctggcg aaatactttg ccgaacgcgc
    24901 gccagcaggg ggagaagcgc atcaactggc gcaggtgatg gcgaaagaag ccgaccgttt
    24961 aaaccgcgtg gtaagcgagt tgctggaact ggttaagcca acgcatctgg ctttgcaggc
    25021 ggtggatctc aacacgctga ttaaccactc attacagctg gtaagccagg atgcaaacag
    25081 ccgggagatc cagttacgct ttaccgccaa cgacacatta ccggaaattc aggccgatcc
    25141 ggacaggctg actcaggtcc tgttgaatct ctatctcaat gctattcagg cgattggtca
    25201 gcatggcgtg attagcgtga cggccagcga aagcggcacg ggtgtgaaaa tcagcgttac
    25261 cgacagcggt aagggaattg cggcggatca gcttgaagcc atcttcaccc cgtatttcac
    25321 cacgaaggcc gaaggcaccg gactggggct ggcggtcgtg cataatattg ttgaacaaca
    25381 cggtggtaca attcaggtcg caagccaggt gggaaaaggc gcaacgttca ccctctggct
    25441 tccggtcact attacgcgta aggacccaca aggatgacgc acgataatat cgatattctg
    25501 gtggtggatg atgacattag ccactgcact attttgcagg ctttgctgcg cggctggggc
    25561 tataacgtcg cgctggcgaa cagcgggcga caggcgctgg agcaggtgcg ggaacaggtt
    25621 tttgatcttg tgctttgcga tgtgcgaatg gcggagatgg acggcattgc cacgctaaaa
    25681 gagatcaaag cgctaaatcc ggcgattccg gtactgatta tgaccgcata ctccagcgtc
    25741 gagacggcgg tagaggcact gaaaaccggg gcgctggatt atctcatcaa gccgctggat
    25801 ttcgataacc tgcaggcgac gctggaaaag gcgctcgcac atacgcacag tgttgacgct
    25861 gaaacgcctg cggtgtctgc cagccagttc ggtatggtag gtaaaagccc ggcgatgcaa
    25921 cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag ccacagtgct gatccacggc
    25981 gattccggca ccggtaaaga gctggtcgca agggcgattc acgccagtag cgcacggagt
    26041 gaaaaaccac tggtaacgct caactgtgcg gcactcaacg aatccttgct ggaatctgaa
    26101 ttgttcggtc acgaaaaagg ggcgtttacc ggagccgata aacgccggga agggcgcttt
    26161 gttgaggcgg acggcggcac gctgtttctc gatgaaattg gcgatatctc gccgatgatg
    26221 caggttcgtc tgctgcgtgc gattcaggag cgcgaagttc agcgtgtcgg cagcaaccag
    26281 accatctcgg ttgatgtccg gctgattgcg gcgactcatc gcgatcttgc cgcagaggtg
    26341 aatgccgggc gttttcgcca ggatctctac tatcgcctga acgtggtggc gattgaagtt
    26401 ccgtcgctgc gtcagcggcg ggaagatatt cctctgctgg caaaccattt tcttcagcgc
    26461 tttgccgagc gtaatcgtaa ggcggtaaaa ggttttacgc cccaggcgat ggatctgctg
    26521 attcactacg actggccggg aaatattcgt gaactggaaa acacggtgga acgggcggtg
    26581 gtgctgctga ccggggaata tatttccgaa cgcgagctgc cgctggcgat tgcgagtacg
    26641 ccgatcccgc tggtacaaag tcaggatatt cagccgctgg tggaagtcga aaaagaggtg
    26701 attctggcgg cgctggagaa aacgggcggc aacaaaaccg aagccgcccg ccagttaggg
    26761 atcacgcgca aaacgctatt ggcaaaactg tcgcgttagt tctgctcgcg ttcgatagcg
    26821 cgccagccga tatctttccg gcagaagcag tcgtcccaat ggatatcggt cattaaggca
    26881 taagcgcgtt tctgcgcttc tgccacggta tgacccagcg cggtaacgca cagtacgcgc
    26941 ccgccgctgg tcactacctg ctcgtcatcc gctagtttcg tgcccgcgtg gaacactttg
    27001 ccgtctgcca cttcttccag cggcaggccg tggatcacat cgccggtgcg gtaatcgccc
    27061 ggatatccac ccgcagccat caccacgccg agagaagcgc gttcatccca ctcggacgtt
    27121 ttctcgtcca gcttgccttc acaggccgcc aggcagagct caaccagatc ggacttcatg
    27181 cgcagcataa tcggctgggt ttctggatcg ccaaagcggc agttaaattc aataaccttc
    27241 ggattgccct gtttgtcgat catcaggccc gcgtagagaa aaccagtgta ggtgttgcct
    27301 tctgccgcca tgcctttcac ggttggccag atgattcgtt ccatcgtgcg ctgatgaact
    27361 tcatcggtta ctaccggcgc gggggagtaa gcgcccatcc cgcccgtgtt tggtccggta
    27421 tctttatcgc ctacgcgttt gtgatcctgg ctggtagcca tcggcagcac atgctcgccg
    27481 tccaccatca cgataaagct cgcttcttcg ccatcgagga actcttcgat aacgatgcga
    27541 tgacccgcgt cgccaaacgc gttgcccgcc agcatatcgt gaacagctgc ttcagcttct
    27601 tccagcgtca tcgccacgat aacgcctttc ccggctgcca gaccgtcagc tttaatgacg
    27661 attggcgcgc ctttctcacg cagatacgcc agcgcaggtt ctacctcggt gaagttctgg
    27721 tattccgctg tgggaatgtt atggcgggcc agaaagtctt tggtgaatgc tttagaacct
    27781 tccagttgtg cggcacctgc ggttgggccg aagattttca gcccggcggc gcggaaggta
    27841 tcgactacgc ctttcaccag cggcgcttcc gggccgacga tggttagatc gatcttttcg
    27901 ttttgcgcga aatccagcag cgccgggata tcggttacgc cgatagcgac gttttgcagt
    27961 gtgggttcca gcgcagtgcc tgcattaccc ggagcaacaa aaacagtttc aaccagcggc
    28021 gactgggccg ctttccaggc cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact
    28081 ttcatctgtc gctccattaa tggcggaagt ggcgcatgtc ggtgaagagc atcgcaatac
    28141 cgtgctcgtc ggcggcggca atcacttcgt catcacggat agaaccgcca ggctggatta
    28201 cgcaggtcac acccgcagcg gcggcggcat caataccgtc gcggaacggg aagaacgcgt
    28261 cagaagccat cgaggaacct ttcacttcca ggccttcatc ggccgcttta ataccggcga
    28321 tttttgcgga gtacacgcgg ctcatctggc ccgcgccaat gccgatggtc atattgtttt
    28381 tggcatagac aatggcgttg gatttcacga acttcgccac tttccagcag aacagcgcat
    28441 cacgcagttc ctgttcggtc ggctgacgtt tggtgacgac gcgcagttct tctgcaccga
    28501 ccatgcccag gtcgcgatcc tgaaccagca gaccgccgtt aacacgtttg aaatcaagac
    28561 ccggaacacg ctcgccccac tgaccgcagg tcaggacgcg tacgttctgt ttggcggcgg
    28621 tgattttcag ggcttcttcg ctggcggacg gcgcaataat cacttcaaca aactgacgag
    28681 aaatgatggc ctgcgcggtt tccgcatcca gctcgcggtt aaaggcgata atgccgccga
    28741 atgcggaggt cgggtcggtt ttgtacgcgc gatcgtaagc atcaagaatg gaattgctga
    28801 tagccacgcc gcacgggttg gcgtgcttca caatcacaca tgctggctcg gcgaactctt
    28861 tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt ataagagagg gccttaccct
    28921 gaacctgggt tgcggtggca acagaggctt ctttcacgtt ctcttctata tagaaggcag
    28981 cctgctggtg gctgttctcg ccgtaacgca tatcctgctt cttaatgaag ttcaggttca
    29041 gcgtgcgtgg gaagcgaccg gcggcttctt tgctttcacc gtggtaagcc ggaaccatgc
    29101 tgccgaagta gttggcaatc atgctgtcgt aggcggcggt atgttcaaag gctttgatag
    29161 cgagatcgaa acgggttgca agcagaagcg atccttcgtt ggcatccatc tctttaatga
    29221 tggcgtcgta gtcgctgctc ttcaccacga tggcgacatc tttatggttc ttggcggcag
    29281 agcgcaccat cgtcgggccg ccgatatcga tgttctcaac cgcatcttcc agcgagcaac
    29341 cttcacgggc aacggtctgg gcgaacggat acaggttaac aaccaccata tcgataggct
    29401 ggatctgatg ttcttccata atggcatcgt cctggccgcg acggcccaga atgccaccat
    29461 gtactttcgg atgcagggtc ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat
    29521 cggaaacttc ggttaccggc agaccttttt ctgctaacag acgggctgtg ccccctgtag
    29581 acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt
    29641 cagaaacact gagcagagcg cggcggactg gacgacgttg ttgcatggta aatcccctgg
    29701 atttgactat tacagagagc gttagctgaa tttttcgcga aaaactcagc taacgcccct
    29761 aacggggcat ccttattttt cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga
    29821 atttttctct ttcaatggtg atcacaattt tgacagtggc taccgtggga aaaatacaga
    29881 aattacattg atgattgtgg ataactctgt gcgtaaaaag gtataaagcg gggttttgct
    29941 ggggaatgca gcagtcagtc atttttatgc aatttttcta ttgcgacctg cggagaactc
    30001 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
    30061 ggttgaagag aaaaatcctg aaattcaggg ttgactctga aagaggaaag cgtaatatac
    30121 gccacctcgc gacagtgagc tgaaagccgc gtcgca
//
LOCUS       DAZUCC010000038        29279 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZUCC010000038 DAZUCC010000000
VERSION     DAZUCC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.038"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            435..1574
                     /gene="wza"
                     /locus_tag="ACHY09_001515"
     CDS             435..1574
                     /gene="wza"
                     /locus_tag="ACHY09_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFS3884503.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1580..2023
                     /gene="wzb"
                     /locus_tag="ACHY09_001516"
     CDS             1580..2023
                     /gene="wzb"
                     /locus_tag="ACHY09_001516"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFS3884504.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFVAVYTLLERSARQWAQALNAEQV"
     gene            2026..4188
                     /gene="wzc"
                     /locus_tag="ACHY09_001517"
     CDS             2026..4188
                     /gene="wzc"
                     /locus_tag="ACHY09_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFS3884505.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGEITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4366..5205
                     /gene="wcaA"
                     /locus_tag="ACHY09_001518"
     CDS             4366..5205
                     /gene="wcaA"
                     /locus_tag="ACHY09_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFS3884506.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5208..5696
                     /gene="wcaB"
                     /locus_tag="ACHY09_001519"
     CDS             5208..5696
                     /gene="wcaB"
                     /locus_tag="ACHY09_001519"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFS3884507.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5693..6910
                     /gene="wcaC"
                     /locus_tag="ACHY09_001520"
     CDS             5693..6910
                     /gene="wcaC"
                     /locus_tag="ACHY09_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFS3884508.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQRVAGKRQLFSEMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQSSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6885..8102
                     /gene="wcaD"
                     /locus_tag="ACHY09_001521"
     CDS             6885..8102
                     /gene="wcaD"
                     /locus_tag="ACHY09_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFS3884509.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8113..8859
                     /gene="wcaE"
                     /locus_tag="ACHY09_001522"
     CDS             8113..8859
                     /gene="wcaE"
                     /locus_tag="ACHY09_001522"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFS3884510.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAHGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8875..9423
                     /gene="wcaF"
                     /locus_tag="ACHY09_001523"
     CDS             8875..9423
                     /gene="wcaF"
                     /locus_tag="ACHY09_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFS3884511.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9450..10571
                     /gene="gmd"
                     /locus_tag="ACHY09_001524"
     CDS             9450..10571
                     /gene="gmd"
                     /locus_tag="ACHY09_001524"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFS3884512.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYDMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10574..11539
                     /gene="fcl"
                     /locus_tag="ACHY09_001525"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10574..11539
                     /gene="fcl"
                     /locus_tag="ACHY09_001525"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFS3884513.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11542..12021
                     /gene="gmm"
                     /locus_tag="ACHY09_001526"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11542..12021
                     /gene="gmm"
                     /locus_tag="ACHY09_001526"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFS3884514.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVVEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            12018..13241
                     /gene="wcaI"
                     /locus_tag="ACHY09_001527"
     CDS             12018..13241
                     /gene="wcaI"
                     /locus_tag="ACHY09_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFS3884515.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWQPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVANADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13244..14680
                     /gene="cpsB"
                     /locus_tag="ACHY09_001528"
     CDS             13244..14680
                     /gene="cpsB"
                     /locus_tag="ACHY09_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFS3884516.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRTAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAIDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14873..16243
                     /gene="cpsG"
                     /locus_tag="ACHY09_001529"
                     /gene_synonym="manB"
     CDS             14873..16243
                     /gene="cpsG"
                     /locus_tag="ACHY09_001529"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFS3884517.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     NNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLADPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16298..17692
                     /gene="wcaJ"
                     /locus_tag="ACHY09_001530"
     CDS             16298..17692
                     /gene="wcaJ"
                     /locus_tag="ACHY09_001530"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFS3884518.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17694..19172
                     /gene="wzxC"
                     /locus_tag="ACHY09_001531"
     CDS             17694..19172
                     /gene="wzxC"
                     /locus_tag="ACHY09_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFS3884519.1"
                     /translation="MNLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDTI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLIVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19833..21113
                     /gene="wcaK"
                     /locus_tag="ACHY09_001532"
     CDS             19833..21113
                     /gene="wcaK"
                     /locus_tag="ACHY09_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFS3884520.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            21110..22330
                     /gene="wcaL"
                     /locus_tag="ACHY09_001533"
     CDS             21110..22330
                     /gene="wcaL"
                     /locus_tag="ACHY09_001533"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFS3884521.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIIALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLCGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            22341..23735
                     /gene="wcaM"
                     /locus_tag="ACHY09_001534"
     CDS             22341..23735
                     /gene="wcaM"
                     /locus_tag="ACHY09_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFS3884522.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            23893..24888
                     /locus_tag="ACHY09_001535"
     CDS             23893..24888
                     /locus_tag="ACHY09_001535"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFS3884523.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLTAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            25131..26024
                     /gene="galF"
                     /locus_tag="ACHY09_001536"
     CDS             25131..26024
                     /gene="galF"
                     /locus_tag="ACHY09_001536"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFS3884524.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            26396..27481
                     /gene="rfbB"
                     /locus_tag="ACHY09_001537"
     CDS             26396..27481
                     /gene="rfbB"
                     /locus_tag="ACHY09_001537"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS3884525.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            27481..28380
                     /gene="rfbD"
                     /locus_tag="ACHY09_001538"
     CDS             27481..28380
                     /gene="rfbD"
                     /locus_tag="ACHY09_001538"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS3884526.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEQCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVATTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTVI"
     gene            28438..>29279
                     /gene="rfbA"
                     /locus_tag="ACHY09_001539"
     CDS             28438..>29279
                     /gene="rfbA"
                     /locus_tag="ACHY09_001539"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS3884527.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFHQLLGDGGQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKKGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGGLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNA"
BASE COUNT         7304 a         7112 c         7855 g         7008 t
ORIGIN      
        1 ccatattgaa tgacacttaa tataattctt aaaaatagcc aattgccgaa ttgttacctt
       61 gcctgctatt ccgttagctg taacacttcc tcctgcatta ttggagagcc aatattcaat
      121 taccataatg tcaccttata ttcggaagtt aatttccgca ataacccttt ctcatcaacg
      181 actgtatgtt gagagggttt taaaaattaa gctaaatcag tgtattggta gctaaaaagc
      241 caggggcggt agcgtgtctg gatgcctgaa agaccagtct gaattattct gtcaatagcc
      301 tgcggatgaa atcaattttt ttaggactga tgccagttaa atttttattc aattaactgc
      361 aaggtaaatg tgcatggcga cattgctatt aatagtgcac aggataatta ctctgccaaa
      421 gtgataaata aacaatgatg aaatccaaaa tgaaattgat gccattattg gtgtcagtaa
      481 ccttgataag cggttgcaca gtacttccgg gcagcaatat gtcgacgatg ggtaaagacg
      541 tcatcaaaca gcaggacgct gatttcgatc tcgacaaaat ggtgaatgtt tatccgttga
      601 ccccgcgcct gattgatcaa ttacgcccac gcccgaatgt agcgcgcccc aatatgacgc
      661 tggaaagtga gatcgcgaat tatcagtatc gcgtcgggcc gggggacgtt cttaatgtca
      721 ccgtctggga tcacccggaa ctcaccacgc cagccggtca gtaccgcagc tccagcgaca
      781 ccggcaactg ggtacagcct gacggcacca tgttctaccc gtatatcggc aaggttcacg
      841 tcgtggggaa aacgctcgct gaaatccgca gtgatattac cggtcgctta gcgacgtaca
      901 tcgctgaccc gcaggtggac gttaatatcg ccgccttccg ctcgcaaaaa gcctatatct
      961 caggtcaggt gaataaatcc ggtcaacagg cgattaccaa cgtaccactg accattctcg
     1021 atgccatcaa cgccgcaggt ggcctgaccg acaccgctga ctggcgcaac gtggtgctaa
     1081 cacacaatgg tcgtgaagaa cgcatttctt tgcaggcgct gatgcaaaac ggcgacctca
     1141 atcagaaccg actgctttac cccggcgata ttctctacgt gccgcgtaat gatgatctga
     1201 aagtgtttgt gatgggtgaa gttaaaaaac agagcaccct gaaaatggac tttagcggca
     1261 tgaccctaac tgaagccctg ggtaatgctg aaggcatcga catgaccacc tccaacgcca
     1321 gcggcatctt tgtcattcgt ccgttgaaag gcgagggcgg gcgtaacggc aagattgcca
     1381 atatctacca gctggatatg tccgatgcca catcgctggt gatggcgaca gaattccgcc
     1441 tgcaacctta tgacgtggtg tatgtcacca ccgccccggt ttcccgctgg aaccgtctga
     1501 tcaatcagtt gctgccaact attagcggtg tccgttacat gacggataca gccagcgaca
     1561 ttcataactg gtaatcgtca tgtttaacaa catcttagtt gtctgtgtcg gcaatatttg
     1621 ccgttccccg acggcggaac gcttactgca acgttatcac ccggagctga aagtggagtc
     1681 cgctggactc ggcgcgctgg tcggtaaggg cgctgaccct actgctatca gcgtcgccgc
     1741 agaacatcaa ctgtctctgg aaggtcactg tgcacggcaa atcagccgtc gcctgtgtcg
     1801 caactacgac ctgattttga ccatggaaaa gcgccatatc gaacgcttat gcgagatggc
     1861 accggagatg cgcggcaaag tgatgctgtt tggtcactgg gataacgaat gtgaaatccc
     1921 cgatccgtat cgcaaaagcc gggaaacgtt tgtagcggtg tacacattac ttgaacggtc
     1981 tgcccgccag tgggcgcagg cattgaacgc agagcaggta taagaatgac agaaaaagta
     2041 aaacaacatg ccgctccggt aacgggcagt gatgaaatcg atattggtcg cctggtcggc
     2101 accgtcattg aagcgcgctg gtgggtgatt ggcatcaccg ccgtatttgc cctctgtgcc
     2161 gtggtttaca ccttcttcgc tacgccgata tacagcgccg acgcactggt acaaatcgaa
     2221 caaagcagcg gcaattcgtt agtgcaggat atcggatcgg cattagccaa caaaccgcct
     2281 gcatcggacg ccgagatcca gttgattcgt tcgcgcctgg tgcttggtaa aacggtggac
     2341 gatctcgacc tcgatattgc ggtgagcaaa aacacgttcc cgattttcgg tgcgggctgg
     2401 gatcgcctga tgggacgcca gaacgagacg gtaaaagtga ccacctttaa ccgtcctaag
     2461 gagatggcgg atcaggtgtt tacgcttaat gtgcttgatg acaaaaacta cactttgagc
     2521 agcgatggcg gctttagcgc ccgtgggcaa gcgggccaga tgctgaaaaa agaaggcgtc
     2581 acgctgatgg ttgaagctat tcacgccagc ccgggcagtg agtttaccgt caccaaatac
     2641 tccacgctgg ggatgatcaa tcaactgcaa aacagcctga cggtaacgga gaacggcaaa
     2701 gatgctggcg ttttgagcct gacttatact ggagaagatc gcgaacagat ccgcgacatt
     2761 cttaacagta tcgcccgtaa ctatcaggaa caaaatattg agcgcaaatc ggcggaagcg
     2821 tcgaaaagcc tcgctttcct cgcgcaacag ttaccggaag tacgtagccg cctcgatgtt
     2881 gccgaaaaca aactgaatgc cttccgtcag gataaagatt cagttgatct gccgctggaa
     2941 gcgaaagcgg tgctcgattc gatggtgaac atcgacgcgc agttgaacga actgaccttt
     3001 aaagaagcgg aaatctccaa gctgtacacc aaagttcacc cggcgtatcg cacgctgctg
     3061 gagaaacggc aggcgctgga agacgaaaaa gccaaactta atggtcgcgt aacggcgatg
     3121 ccgaaaaccc agcaggaaat tgtccgtctg acccgcgatg tcgagtctgg gcagcaggtc
     3181 tatatgcaac tgctgaataa agagcaggag ctgaaaatca ccgaagccag caccgtcggc
     3241 gatgtgcgca ttgttgaccc ggcaatcacc cagcctggtg tgctaaaacc gaagaaaggg
     3301 ctgattatcc ttggggcgat tatccttggc ctgatgctct ctatcgtggg tgtgctgctg
     3361 cgctcgttgt ttaatcgcgg tatcgaaagt ccgcaggtgc tggaagaaca cggtatcagc
     3421 gtctatgcca gcatcccgct gtcggagtgg cagaaagcgc gcgatagcgt caaaaccatc
     3481 aaagggatta aacgctataa acagagccag ctactggcgg tggggaatcc aaccgatctg
     3541 gcgattgaag ccatccgtag tctgcgcacc agtctgcact tcgcgatgat gcaggcacag
     3601 aacaatgtgt tgatgatgac cggggttagc ccgtcaatcg gtaaaacctt tgtctgcgcc
     3661 aacctggcgg cggtgatcag tcagaccaat aaacgggtgt tgttgatcga ctgcgatatg
     3721 cgcaaaggct acacccacga gctgttgggc actaataacg tcaatggcct gtcggaaatt
     3781 ctgattggtc agggcgagat tactaccgct gctaaaccga cctctattgc caaatttgac
     3841 ctgatcccgc gcggtcaggt accgccaaat ccttcggaac tgttgatgag cgaacgtttt
     3901 gccgaactgg tgaactgggc gagtaaaaac tacgacctgg tgttgattga tacgccgccg
     3961 attctggcag tgaccgatgc ggcaattgtt ggtcgtcatg tcggaaccac gttgatggtg
     4021 gcgcgttatg cggtcaacac attgaaagaa gtggaaacca gtctgagccg ctttgagcaa
     4081 aacggtattc cggtgaaagg ggtgattctg aactccatct tccgtcgcgc cagcgcgtat
     4141 caggattatg gctattacga atacgaatat aagtcggacg cgaaataacg aaatccgctg
     4201 tcggatgcga tgctgacgca tcttatccga cctacgttca cgccgcatca ggcaagcaaa
     4261 cccgctccgt aggtcggata aggcgttcac gccgcatccg gcaacagaac ctgtaaacca
     4321 gcttataaac cggcagaaca tctcgacact ccgggagaac aacctatgaa agacaatccg
     4381 ctgatttcaa tctatatgcc gacctggaac cgtcaacaac tggcgattcg ggcgataaaa
     4441 tcggtcctgc gccaggacta cagtaactgg gagatgatca tcgtggatga ttgctccacc
     4501 tcctgggaac aactacaaca gtacgtcacc gccctcaacg atccgcgtat cacttacatt
     4561 cacaacgaca ttaacagcgg ggcgtgcgcg gtacgtaacc aggcgattat gctggcgcaa
     4621 ggggaatata tcaccgggat cgatgatgat gatgaatgga cacccaaccg tctgagcgtc
     4681 ttcctcgccc ataaacagca actggtcaca cacgcctttt tgtacgctaa cgactacgtt
     4741 tgccagggcg aagtctattc ccaaccggca agtttgccac tgtatcccaa atcgccgtat
     4801 tcacgccgcc tgttctacaa acgcaatatc attggcaatc aggtctttac ctgggcgtgg
     4861 cgtttcaaag agtgtttgtt cgataccgaa ctgaaagcgg cacaggatta cgacattttc
     4921 ctgcggatgg tggtggagta tggcgaaccg tggaaagtgg aagaggcgac gcagatcctg
     4981 catatcaatc acggcgaaat gcagatcacc tcgtcaccga aaaaattctc cggatacttc
     5041 cacttttacc gtaagcacaa agacaaattt gaccgcgcca gcaaaaaata tcagctgttt
     5101 accctctacc agatccgcaa taaacgcatg acctggcgca ccttgttgac gctgctgtcg
     5161 gtgcgtaacg gcaaacgtct ggctgatggt attcgggggc gctaaagatg ctggaagatc
     5221 tgcgcgccaa cagctggagt ttacgcccgt gctgcatggt tcttgcctat cgcgccgctc
     5281 atttttgttc ggtgtggcgc aaaaagaacg tcctcaacaa tctttgggcg gccccgctgc
     5341 tggtgctgta tcgcattatc accgaatgct ttttcggtta tgaaatccag gccgccgcga
     5401 ccattggccg ccgtttcact atccatcacg gttacgccgt agtgatcaat aaaaacgtgg
     5461 tagcggggga tgatttcacc attcgccacg gcgtcactat cggcaatcgt ggtgccgata
     5521 acatggcatg tccacacatt ggcaacggcg tcgaactcgg tgccaacgtc attattcttg
     5581 gtgatatcac gcttggtaac aacgtcaccg tgggcgcggg cagcgtggtg ctcgactctg
     5641 tcccggacaa cgcgctggtg gtgggagaaa aagcgcgagt gaaggtaatt aaatgaatat
     5701 tctgcaattc aatgtgcgac tggcggaagg cggggcggca ggtgtggcgt tagatctcca
     5761 ccagcgcgcg ctgcaacagg ggctggcgtc acattttgtc tacggctacg gcaaaggcgg
     5821 caaagagagc gtcagccatc aaaactatcc gcaggtcatc aaacatacgc cgcggatgac
     5881 cgcgatggcg aacattgcgt tgtttcgtct gcttaatcgc gatctgttcg gcaatttcaa
     5941 tgagttatat cgcaccatta ctcgcacacc gggtccggtg gtcctgcatt ttcatgtgct
     6001 gcacagctac tggttgaatc ttaagagcgt ggtgcgcttt tgcgaaaaag tgaaaaacca
     6061 taaaccggat gtcactctgg tctggacgtt acacgatcac tggagcgtta ccggacgctg
     6121 cgcctttacc gacggttgcg aaggctggaa aacgggctgc cagaaatgcc cgaccttaaa
     6181 taattatccg ccggtgaaga ttgatcgcgc acaccaacgg gtggcgggca aacggcagtt
     6241 attcagcgag atgctggcgc tgggctgtca gtttatttcc cccagccagc atgtggcaga
     6301 cgctttcaat agtctgtacg gtccagggcg ttgccggatt atcaataacg gtattgatat
     6361 ggcaaccgaa gcgattctgg cggacttgcc tccggtgcgc gaaacccagg gcaagccgaa
     6421 aatcgcggtg gtggcgcatg acctgcgtta cgacggcaaa actaaccagc aactggtacg
     6481 cgagatgatg gcgctgggcg acaaaatcga actgcatact ttcggtaagt tctcgccgtt
     6541 caccgctggt aacgttgtta atcacggctt tgaaactgac aaacgtaaac tgatgagcgc
     6601 gctcaaccag atggatgcgc tggtgttcag ttctcgcgtc gataactacc cgctgatttt
     6661 gtgtgaggcg ttatcgattg gcgtgccggt gattgccacc catagcgacg cggcgcggga
     6721 agtgctgcaa aaatccggcg gtaaaaccgt cagcgaagaa gatgtgctgc aactggtgca
     6781 gtcaagcaaa ccggaaatcg cacaggcgat atttggtacc acgctggctg agttcagcca
     6841 gcgcagccgc gccgcctaca gtggacaaca gatgctggag gagtatgtca acttctatca
     6901 gaatctgtag ctacctgctg ctgccgctga tctacctgct ggttaacgtc aaaatcgccc
     6961 agcttggcga aagttttccc atcaccatcg tcactttttt acctgtcttg ttactgctgt
     7021 ttttagaacg catcagcgtt aaaaaattga tgattgcctt agggattggt gcgggactca
     7081 cggcgtttaa ctacctgttt ggtcagtcgt tggatgccgg taaatacgtc acttcaacta
     7141 tgctgtttgt ctatattgtg atcattattg gcatggtgtg gagtattcgc tttaaaacga
     7201 tttcgcccca caaccaccgc aagatattac gtttctttta tctggtggtc gggctggtgg
     7261 tggtgctggc ggcggtggag atggcgcaaa ttatcctcac cggcggtagc agtattatgg
     7321 agtcgatttc gaaatatctg atttacagca acagctatgt gctgaatttc attaaattcg
     7381 gcggcaagcg cacgacagca ctttatttcg aaccggcatt tttcgctctg gcattaatct
     7441 caatttggct cagcatcaaa cagtttggta tcaaaacgcc taaaaccgat gctatgattc
     7501 ttgcagggat aatattatcc ggatcgtttt caggggttat gacctttatc ctgttttatt
     7561 tgctggagtg ggcatttcaa tatctgaata aagaggcgat taagaaaaag ttaccgttag
     7621 cattgatttc tctggctgta ttcctggttg gtgtggtaat cgcgtttcct tatatttcca
     7681 cccgtctggg cgatttaggt acggaaggat cgtcatcgta ttatcgtatt gtcggtccgc
     7741 tggtgatggt cggttattct ttgacccata ttgacggtgt agtcagattt ggctcacttt
     7801 atgaatatgt cgcatcattc ggaatattta acggtgcgga tgtcggaaaa accatagaca
     7861 atggtttgta tctgctgatt atttattttt cctggttcgc agtgttttta tcgctgtggt
     7921 acatggggaa agtgataaaa atgatgatca acgcttttgg tgataaccgc aattttcgcg
     7981 tgcaattata tctttttact ccggtatcgc tgttttttac cggttcgata tttagcccgg
     8041 aatatgcatt tttaatcgtc tgtccgttta ttttgcgaaa agcgttaaat attacgaggt
     8101 aagaataaga acatgttgct tagcataatc actgtcgcgt ttcgtaacct cgcagggata
     8161 gtcaaaacac atgcctcgtt ggcgcatctg gcgcaggctg acgatatcag cttcgaatgg
     8221 attgttgtcg atggcggttc caatgacggc acccgtgagt atctggaaaa tctcaatggt
     8281 atctataacc tacgctttgt cagcgagcca gataacggta tctacgacgc catgaacaaa
     8341 ggcattgcga tggcgcatgg caagttcgcg ttgtttctca actcaggcga tatttttcat
     8401 caggatgccg caaattttgt ccgtaagtta aaagtgcaaa aagataacat gatgatcacc
     8461 ggcgatgcgc tgctggattt tggcgacggg cataaaatta aacgtagcgc caaaccgggt
     8521 tggtatattt atcacagcct gcccgccagt catcaggcga tatttttccc ggtgtccggc
     8581 ttgaaaaaat ggcgttatga cctggaatat aaagtttctt ccgactacgc gctggcagcc
     8641 aaaatgtata aagccggtta tgcatttaaa aaactcaatg gcctggtgtc tgaattttct
     8701 atgggtgggg tatcaaccac caataatatg gaattgtgtg ctgacgccaa aaaagtccaa
     8761 cggcaaatat tacatgtgcc tggcttctgg gcagaattat cctggcattt acgccaacgt
     8821 actacctcaa agacgaaagc cttatataac aaaagctgaa tataagggaa aacgatgcaa
     8881 gatttaagcg gtttctcggt accgaaaggg ttccggggtg gcaacgctat taaagtgcaa
     8941 ttgtggtggg cagtacaggc aacaatattt gcctggtcgc cacaagtatt gtatcgctgg
     9001 cgggcatttt tattacgttt attcggcgca aaaataggaa aaaacgtagt tattcgtccg
     9061 tcagtaaaaa ttacctatcc gtggaaatta accttaggtg attacgcgtg ggtcggcgat
     9121 gacgtcaatt tatataccct cggtgaaata accattggcg cacattcggt gatatcgcaa
     9181 aaaagttatt tatgcaccgg tagccacgac catgcaagtc aacatttcac cattaacgcc
     9241 acgcctattg tgattggcga gaaatgctgg ctggcaaccg atgtctttgt tgccccaggc
     9301 gtcacgatcg gcaacggcac cgtcgtgggt gcacgaagca gtgtttttaa atcgcttccg
     9361 gcaaatgtgg tttgccgggg gaatcccgca gtggtgatac gcgaacgcgt tgaaactgaa
     9421 taaattcaaa aaatacagag gaataagaca tgtcaaaagt cgctctcatc accggtgtaa
     9481 ccggacaaga cggttcttac ctggcagagt ttctgctgga aaaaggttac gaggtgcatg
     9541 gtattaagcg tcgtgcatcg tcattcaaca ccgagcgcgt ggatcacatt tatcaggatc
     9601 cgcacacctg caacccgaaa ttccatctgc attatggcga cctgagtgat acctccaacc
     9661 tgacgcgtat tttgcgtgaa gtgcagccgg atgaagtgta caacctgggc gcaatgagcc
     9721 acgttgcggt ttcttttgag tcaccggaat ataccgcaga cgtcgatgcg atgggtacgc
     9781 tgcgcctgct ggaggcgatc cgcttcctcg gtctggaaaa gaaaacccgt ttctatcagg
     9841 cttctacttc tgaactgtac ggtctggtgc aggaaattcc gcagaaagag accacgccgt
     9901 tctacccacg ctctccgtat gcggtcgcca aactgtacgc ctactggatc accgttaact
     9961 accgcgaatc ctacgacatg tacgcctgta acggtattct cttcaaccat gaatccccgc
    10021 gccgtggcga gactttcgtt acccgcaaaa tcacccgcgc aatcgccaac atcgcccagg
    10081 ggctggagtc gtgcctgtat ctcggcaata tggattccct gcgtgactgg ggccacgcca
    10141 aagactacgt aaaaatgcag tggatgatgc tgcaacagga acaaccggaa gatttcgtta
    10201 tcgcgaccgg cgttcagtac tccgtacgtc agttcgtgga aatggcggca gcacagttgg
    10261 gcatcaaact gcgctttgaa ggcacgggtg ttgaagagaa gggcattgtg gtttccgtca
    10321 ccgggcatga cgcgccgggc gttaaaccgg gcgatgtgat tatcgccgtt gacccgcgtt
    10381 acttccgtcc ggcggaagtt gaaacgctgc tcggcgaccc gaccaaagcg cacgaaaaac
    10441 tgggctggaa accggaaatc accctcagag agatggtgtc tgaaatggtg gctaatgacc
    10501 tcgaagcggc gaaaaaacac tctctgctga aatctcacgg ctacgacgtg gcgatcgcgc
    10561 tggagtcata agcatgagta aacaacgcat ttttattgct ggccatcgcg ggatggtcgg
    10621 ttccgccatc aggcggcagc tcgaacagcg cggtgatgta gagctggtat tacgcacccg
    10681 cgacgagctg aacctgctgg acagccgcgc ggtgcatgat ttctttgcca gcgaaagcat
    10741 tgaccaggtc tatctggcgg cggcgaaagt gggcggcatt gttgccaaca acacgtatcc
    10801 ggcggatttc atctaccaga acatgatgat tgagagcaac atcattcacg ccgcgcatca
    10861 gaacgacgtg aacaaactgc tgtttctcgg atcgtcctgc atctacccga aactggcaaa
    10921 acagccgatg gcagaaagcg agttgttgca gggcacgctg gagccgacta acgagcctta
    10981 tgctattgcc aaaatcgccg ggatcaaact gtgcgaatct tacaatcgcc agtacggacg
    11041 cgattaccgc tcagtcatgc cgaccaacct gtacgggccg catgacaact tccacccgag
    11101 taactcccat gtgatacctg cattgctgcg tcgcttccac gaggcgacgg cacagaatgc
    11161 gccggacgtg gtggtatggg gcagcggtac accgatgcgt gaattcctgc acgtcgatga
    11221 tatggcggcg gcgagcattc atgtcatgga gctggcgcac gaagtctggc tggaaaacac
    11281 ccagccgatg ctgtcgcaca ttaacgtcgg cacgggcgtt gactgcacta tccgcgagct
    11341 ggcgcaaacc atcgccaaag tggtgggtta caaaggtcgg gtggtttttg atgccagtaa
    11401 accggatggt acaccgcgca aactgctgga tgtgacgcgc ctgcatcagc ttggctggta
    11461 tcacgaaatc tcactggaag cggggcttgc cagcacttac cagtggttcc ttgagaatca
    11521 agaccgcttt cgggggtaat gatgttttta cgtcaggaag actttgccac ggtagtgcgc
    11581 tccacaccgc ttgtctctct cgactttatt gtcgagaaca gtcgcggcga gtttctgctt
    11641 ggcaaaagaa ccaaccgccc ggcgcagggt tactggtttg tgccgggagg gcgcgtgcag
    11701 aaagacgaaa cgctggaagc cgcatttgag cggctgacga tggcggaact ggggctgcgt
    11761 ctgccgataa cagcaggcca gttttacggt gtctggcagc acttttatga cgataacttc
    11821 tccggcacgg atttcaccac tcactatgtg gtgctcggtt ttcgcttcag agtagtggaa
    11881 gaagagctgt tactgccgga tgagcagcat gacgattacc gctggctgac gccggacgca
    11941 ctgctcgcca gtgacaatgt tcacgctaac agccgcgcct attttctcgc tgagaagcgt
    12001 gccggagtac ccggattatg aaaatactgg tctacggcat taactactcg ccggagttaa
    12061 ccggcatcgg caaatacacc ggcgagatgg tggaatggct tgcggcgcaa ggtcatgagg
    12121 tgcgggttat taccgcaccg ccttactacc cgcaatggca ggtgggcgag aactattccg
    12181 cctggcggta caaacgagaa gagggggccg ccacggtgtg gcgctgcccg ctgtacgtgc
    12241 caaaacagcc gagcaccctg aaacgcctgt tgcatctcgg cagttttgcc gtcagcagtt
    12301 ttttcccgtt gatggcgcaa cgtcgctggc agccggatcg cattattggc gtggtgccaa
    12361 cgctgttttg cacgccggga atgcgcctgc tggcgaaact ctctggggcg cgcacggtgc
    12421 tccatattca ggattacgaa gtggacgcca tgctggggct gggccttgcc ggaaaaggta
    12481 aaggcggcaa agtggcacag ctggcaacgg cgttcgaacg tagcggactg cataacgtcg
    12541 ataacgtctc cacgatttcg cgttcgatga tgaataaagc tatcgaaaaa ggcgtggcgg
    12601 cggaaaacgt catcttcttc cccaactggt cggaaatcgc ccgttttcag catgttgcaa
    12661 atgccgatgt tgatgccctt cgtaaccagc ttggcctgcc ggataacaaa aaaatcattc
    12721 tttactccgg caatattggt gaaaagcagg ggctggaaaa cgttattgaa gctgccgatc
    12781 gcctgcgcga tgaaccgctg atttttgcca ttgtcgggca gggcggcggc aaagcgcggc
    12841 tggaaaaaat ggcgcagcag cgtggactgc gcaacatgca attttttccg ctgcaatcgt
    12901 atgacgcttt acccgcactg ctgaagatgg gcgattgcca tctggtggtg caaaaacgcg
    12961 gcgcggcaga tgccgtattg ccgtcgaaac tgaccaatat tctggcggta ggcggtaacg
    13021 cggtgattac cgcagaagcc cacaccgaac tgggacagct ttgcgaaacc tttccgggca
    13081 ttgcggtttg cgtggaaccg gaatcggtcg aggcgctggt ggcggggatt cgtcaggcgc
    13141 tcctgctgcc caaacacaac acggtggcac gtgaatatgc cgaacgcacg ctcgataaag
    13201 agaacgtgtt acgtcaattt ataaatgata ttcggggata attatggcgc agtcgaaact
    13261 ctatccagtt gtgatggcag gtggctccgg tagccgctta tggccgcttt cccgcgtact
    13321 ttaccccaag cagtttttat gcctgaaagg cgatctcacc atgctgcaaa ccaccatctg
    13381 ccgcctgaac ggcgtggagt gcgaaagccc ggtggtgatt tgcaatgagc agcaccgctt
    13441 tattgtcgcg gaacagctgc gtcaactgaa caaactcacc gagaatatta ttctcgaacc
    13501 ggcagggcgt aacactgcac ctgccattgc gctggcggcg ctggcggcaa aacgtcatag
    13561 cccggagagc gacccattaa tgctggtgct ggcggcggat catgtgattg ccgatgaaga
    13621 cgcttttcgc accgccgtgc gtaatgccat gccgtatgcc gaagcgggca agctggtgac
    13681 cttcggcatt gtgccggatc tacctgaaac cggatatggc tatattcgtc gcggtgaagt
    13741 gtctgcgggt gagcaggata cggtggcctt tgaagtggcg cagtttgtcg aaaaaccgaa
    13801 tctggaaacc gcgcaggcct atgtggcaag cggcgaatat tactggaaca gcggtatgtt
    13861 tctgttccgc gccggacgct atctcgaaga actgaaaaaa tatcgcccgg atattctcga
    13921 cgcctgtgaa aaagcgatga gcgccatcga tccggatctc gattttattc gtgtggatga
    13981 agaagcgttt ctcgcctgcc cggaagagtc ggtggattac gcagtcatgg aacgtacggc
    14041 agatgctgtt gtggtgccga tggatgcggg ctggagcgat gtcggttcct ggtcctcttt
    14101 atgggagatc agcgcccaca ccgccgaggg caacgtttgc cacggcgatg tgattaatca
    14161 caaaactgaa aacagctatg tgtatgccga atctggcctg gtcaccaccg tcggggtgaa
    14221 agatctggtg gtggtgcaga ccaaagatgc ggtgctgatt gccgaccgta acgcggtgca
    14281 ggatgtgaaa aaagtggtcg agcagatcaa agccgatggt cgccatgagc atcgggtgca
    14341 tcgcgaagtg tatcgtccgt ggggcaaata tgactctatc gacgcgggcg accgctacca
    14401 ggtgaaacgc atcaccgtga aaccgggcga aggcttgtca gtacagatgc accatcaccg
    14461 cgcggaacac tgggtagtgg tcgcgggaac ggcaaaagtc actatcgacg gtgatatcaa
    14521 actccttggt gaaaacgagt ccatttatat tccgctgggg gcgacgcatt gcctggaaaa
    14581 cccggggaaa attccgctcg atttaattga agtgcgctcc ggctcttatc ttgaagagga
    14641 tgatgtggtg cgcttcgcgg atcgctacgg acgggtgtaa acgtcgcatc aggcgctgtt
    14701 tgtcggatgc ggcgtgaacg ccttatccga cctacggttc gggtttgtag gcctgataag
    14761 acgcggcagc gtcgcatcag gcaatgaatg cgaaaccgcg atgtaaataa cgacaaaaac
    14821 aaaattggcc gtttcggtca gggccaacta ttgcctgaaa aagggtaacg atatgaaaaa
    14881 attaacctgc tttaaagcct atgatattcg cgggaaatta ggcgaagaac tgaatgaaga
    14941 tatcgcctgg cgcattggtc gcgcctatgg cgaatttctc aaaccgaaaa ccattgtgtt
    15001 aggtggtgat gcccgcctca ccagcgaaac cttaaaactg gcgctggcga aaggtttaca
    15061 ggatgcgggc gtcgatgtgc tggatattgg tatgtccggc accgaagaaa tctatttcgc
    15121 cacgttccat ctcggcgtgg atggcggcat tgaagttacc gccagccata acccgatgga
    15181 ttacaacggt atgaaactcg tgcgcgaggg ggcccgcccg atcagcgggg ataccggact
    15241 gcgcgacgtc cagcgtctgg cagaagccaa cgactttcct cccgttgatg aaaccaaacg
    15301 cggtcgctat cagcaaatca atctgcgtga cgcttacgtt gatcacctgt tcggttatat
    15361 caacgtcaaa aacctcacgc cgctcaagct ggtgatcaac tccgggaacg gcgcagcggg
    15421 tccggtggtg gacgccattg aagcccgctt taaagccctc ggcgcaccag tggaattgat
    15481 caaagtgcac aacacgccgg acggcaattt ccccaacgga attcctaacc cgctgctgcc
    15541 ggaatgccgc gacgacaccc gcaatgcggt catcaaacac ggcgcggata tgggcattgc
    15601 ctttgatggc gattttgacc gctgttttct gtttgacgaa aaagggcagt ttatcgaggg
    15661 ctactacatt gtcggcctgc tggcagaagc gttcctcgaa aataatcccg gcgcgaagat
    15721 catccacgat ccgcgtctct cctggaacac cgttgatgtg gtgactgccg caggcggcac
    15781 cccggtaatg tcgaaaaccg gacacgcctt tattaaagaa cggatgcgca aggaagacgc
    15841 catctacggt ggcgaaatga gcgcccacca ttacttccgt gatttcgctt actgcgacag
    15901 cggcatgatc ccgtggctgc tggtcgccga actggtgtgc ctgaaaggga aaacgctggg
    15961 cgaactggtg cgtgaccgga tggcagcatt tccggcaagc ggtgagatca acagcaaact
    16021 ggcggaccct gttgaggcga ttaaccgcgt cgaacagcat tttagccgcg aggcgctggc
    16081 ggtggatcgc accgatggca tcagcatgac ctttgccgac tggcgcttta acctgcgcac
    16141 ctccaacacc gaaccggtgg tgcgcctgaa tgttgaatcg cgcggtgatg tgccgctgat
    16201 ggaagcgcga acgcgaactc tgctgacgtt gctgaacgag taatgtcgga tcttccctta
    16261 ccccactgcg ggtaaggggc taataacagg aacaacgatg acaaatctaa aaaagcgcga
    16321 gcgagcgaaa accaatgcat cgttaatctc tatggtgcaa cgcttttcag atatcaccat
    16381 catgtttgcc ggactatggc tggtttgcga agtcagcgga ctgtcattcc tctacatgca
    16441 cctgttggtg gcgctgatta cgctggtggt gttccagatg ctgggcggca tcaccgattt
    16501 ttatcgctca tggcgcggtg ttcgggcagc gacagaattt gccctgctgc tacaaaactg
    16561 gaccttaagc gtgattttca gcgccgggct ggtggcgttc aacaatgatt tcgacacgca
    16621 actgaaaatc tggctggcgt ggtatgggct gaccagtatc ggactggtgg tttgccgttc
    16681 gtgtattcgc attggggcgg gctggctgcg taatcatggc tataacaagc gcatggtcgc
    16741 ggtggcgggg gatttagccg ccggacaaat gctgatggag agcttccgta atcagccgtg
    16801 gttagggttt gaagtggtgg gcgtttacca cgacccgaaa ccgggtggcg tttctaacga
    16861 ctgggcgggc aatctgcaac agctggtcga ggatgcgaaa gcgggcaaga ttcataacgt
    16921 ctatatcgcg atgcaaatgt gcgacggcgc acgcgtgaaa aaactggtcc atcaattggc
    16981 ggacaccacc tgttcggtgc tgctgatccc cgatgtcttt accttcaaca ttctccattc
    17041 ccgtatcgaa gagatgaacg gcgtaccggt ggtgccgctg tatgacacgc cgctttccgg
    17101 ggttaaccgc ctgctcaaac gtgcggaaga tattgtgctg gcgacgctta tcctgctgct
    17161 gatctccccg gtgctgtgct gcattgcgct ggcggtgaaa ctgagctcac cggggccggt
    17221 tattttccgc cagactcgct acggcatgga cggcaagccg atcaaagtgt ggaagttccg
    17281 ttccatgaaa gtgatggaga acgacaaagt ggtgacccag gcgacgcaga acgatccgcg
    17341 cgtcaccaaa gtggggaact ttctgcgccg cacctcgctg gatgaattgc cgcagtttat
    17401 caatgtgctg actggcggga tgtcgattgt cgggccacgt ccgcacgcgg tggcgcataa
    17461 cgaacagtat cgccagctca ttgaaggcta catgctgcgc cataaagtga aaccgggcat
    17521 taccggctgg gcgcagatta acggctggcg cggcgaaacc gacacactgg agaaaatgga
    17581 aaaacgcgtc gagttcgacc ttgagtacat ccgcgaatgg agcgtctggt tcgatatcaa
    17641 aatcgttttc ctgacggtat tcaagggctt cgttaacaaa gcggcatatt gatatgaact
    17701 tacgtgaaaa aaccatcagc ggcgcgaagt ggtcggcgat tgccacggtg atcatcatcg
    17761 gcctcgggct ggtgcagatg accgtgctgg cgcggattat cgacaaccac cagttcggtt
    17821 tgcttaccgt gtcgctggtg atcatcgcgc tggcagatac gctttctgac ttcggcatcg
    17881 ctaactcgat tattcagcga aaagaaatca gccaccttga actcaccacg ctgtactggc
    17941 tgaacgtcgg gctggggatc gtggtgtgcg tggcggtgtt tttgttgagt gataccattg
    18001 gcgacgtgct gaataacccg gacctggcac cgttgattaa aacattgtcg ctggcgtttg
    18061 tggtgatccc ccacgggcag cagttccgcg cgttgatgca gaaggagctg gagttcaaca
    18121 aaatcggcat gatcgaaacc agtgcggtgc tggcgggctt cacttttacg gtggttagcg
    18181 cccatttctg gccgctggcg atgaccgcga tcctcggtta tctggtcaat agtgcggtaa
    18241 gaacgctgct gtttggctac tttggccgca aaatttatcg ccccggtctg catttctcgc
    18301 tggcgtcggt ggcaccaaac ttacgttttg gtgcctggct gacggcggac agcatcatca
    18361 actatctcaa taccaacctt tcaacactcg tgctggcgcg tattctcggc gcgggcgtgg
    18421 cagggggata caacctggcg tacaacgtgg ccgttgtgcc gccgatgaaa ctgaacccca
    18481 tcatcacgcg cgtgttgttt ccggcatttg ccaaaattca ggacgacacc gaaaagctgc
    18541 gcgtcaactt ctacaagctg ctgtcggtgg tggggattat caactttccg gcgctgctgg
    18601 ggctgatagt ggtgtcgaat aactttgtac cgctggtatt tggtgagaag tggaacagca
    18661 ttattccggt gctgcaattg ctgtgtgtgg tggggctgct gcgctcagtg gggaacccga
    18721 ttggttcgct gctgatggcg aaagcgcggg tcgatatcag ctttaaattc aacgtattca
    18781 aaacctttct gtttattccg gcgattgtta ttggtgggca gatggcgggc gcaatcggcg
    18841 tcacgcttgg cttcctgctg gtgcagatta tcaacaccat tctgagctat ttcgtgatga
    18901 ttaaaccggt gctcggttcc agttatcgcc agtacatcct gagtttgtgg ctgccgtttt
    18961 atctctcgct gccgacgctg gtggtcagtt atgcgctggg cattgtgctg aaagggcaac
    19021 tggcgctggg gatgctgctg gcggtgcaaa tagccacggg cgtgctggcg tttgtggtga
    19081 tgattgtgct gtcgcgccat ccgctggtgg tggaagtgaa gcgtcagttt tgtcgcagcg
    19141 aaaaaatgaa aatgctttta cgggcggggt gaatggttgt tttcggttcc ggcaacggtt
    19201 gccggatgcg gcgtaaaacg ccttatccgt cctggggggc tgggcttgga tcttgtaggc
    19261 ctgataagac gcgccagcgt cgcatcaggc actgaatgcc ggatgcggcg tgaacgcctt
    19321 atccggccta cggttctgtg ctcggatctt gtaggcctga taagacgcgt cagcgtcgca
    19381 tcaggcactg aatgccggat gcggcgtaaa acgccttatc cgtcctacgg ctctgtgctc
    19441 gggtcttgta ggcctgataa gacgcgtcag cgtcgcatca ggcactgaat gccggaagcg
    19501 gcgtgaacgc cttatccgtc ctacggttct gggcttggat cttgtaggcc tgataagagg
    19561 cgtcagcgtc gcatcaggta ctgaatgccg gatgcggcgt gaacgcctta tccgtcctac
    19621 ggttctgggc ttggatcttg taggcctgat aagacgcgtc agcgtcgcat caggcactga
    19681 atgccggatg cggcgtgaac gccttatccg tcctacggtt ctgtgctcgg atcttgtagg
    19741 cctgataaga cgcgttagcg tcgcatcagg caaaaaacgg caacgaacac aacaaacaca
    19801 gcctcacctg gcatcaaatt tatgaggaaa aaatgaaatt acttattctg ggcaaccaca
    19861 cttgcggcaa tcgtggcgat agcgccatcc tgcgtggctt acttgatgcc atcaatatcc
    19921 tcaatccaca tgccgaagtg gacgtgatga gccgctatcc ggtcagttct tcctggctgc
    19981 tcaaccgccc ggtaatgggc gatccgctgt tcctgcaaat gaaacaacac aacagcgcgg
    20041 cgggcgttgt cgggcgtgtt aaaaaagtcc tccgtcgccg ctaccagcac caggtattgc
    20101 tctcacgcgt caccgacact ggcaagctgc gtaatatcgc catcgcccag ggattcactg
    20161 acttcgtgcg actgctgtca ggttacgacg ccattattca ggtcggcgga tcgttttttg
    20221 tcgatctcta cggcgtgccg cagtttgaac atgcgctttg cactttcatg gcgaaaaagc
    20281 cgctgtttat gattggtcac agcgtcggcc ccttccagga tgagcaattt aaccaactgg
    20341 cgaactatgt ctttggtcac tgcgacgcgc tgatcctgcg cgaatcggtc agccttgatt
    20401 tgatgaaacg cagcaatatc accactgcaa aagtggaaca tggcgtcgat accgcgtggc
    20461 tggtcgatca ccacattgaa gacttcaccg ccagctatgc tgttcaacac tggctggatg
    20521 ttgccgcaca acagaaaacg gtggcaatta ccctgcgcga actggcaccg ttcgacaaac
    20581 gtctcggcac cactcaacaa gcgtatgaaa aagcctttgc cggggtggtc aatcgcattc
    20641 tcgatgaagg gtatcaggtg attgcgctct ccacctgtac gggcatcgac agttataaca
    20701 aagacgaccg catggtggcg ctcaatctgc gccagcacat cagcgatcct gcccgttatc
    20761 acgtagtgat ggatgaactc aacgatctgg aaatgggcaa aattctgggg gcctgtgaac
    20821 ttaccgtcgg tacgcgcctg cactctgcta ttatctcgat gaactttgcc actccggcga
    20881 ttgccatcaa ctacgaacat aaatccgccg ggatcatgca acaactgggg ctaccagaaa
    20941 tggcgattga tatccgtcat ttattagacg gcagcctgca agcgatggtt gcggatacct
    21001 taggccagct tccggcactg aacacgcgac ttaacgaagc cgtcagtcgc gaacgtcaga
    21061 cgggaatgca gatggtgcaa tctgtgcttg aacgcatcgg ggaggtgaaa tgaaggtcgg
    21121 cttcttttta ctgaaatttc cgttgtcgtc ggaaaccttc gttcttaacc aaatcactgc
    21181 gtttattgat atgggatttg aggtggagat tatcgcgctg caaaaaggcg acacacaaaa
    21241 cacccacgcg gcatggacga aatacaacct tgctgccaga acccgctggt tacaggacga
    21301 acctacgggc aaagtggcga aactgcgcca ccgcgccagc cagaccttat gcggcattca
    21361 tcgtaaaaat acctggcagg cgctcaacct caaacgctat ggcgccgagt cgcggaacct
    21421 gattttgtct gctatttgcg gtcaggtcgc aacaccgttt catgccgatg tctttatcgc
    21481 tcactttggt ccggcagggg taaccgcagc aaaactccgc gaactgggtg tcattcgcgg
    21541 caaaattgcc actatcttcc acggtattga tatctccagt cgggaggtgc tcaaccatta
    21601 cactcccgaa tatcagcaac tgttccgccg tggcgacctg atgttaccga taagcgatct
    21661 gtgggccgga aggctgcaaa aaatgggctg cccgagggaa aaaatcgccg tatcgcgtat
    21721 gggcgtggac atgacgcgct ttagcccgcg tccggtgaaa gcgcctgcaa cgccgctgga
    21781 aatcatttcc gtcgcacgct taaccgagaa aaaaggcctg catgtggcga tcgaagcctg
    21841 ccgtcagttg aaagagcagg gcgtggcatt tcgctatcgc atcctcggca ttggcccgtg
    21901 ggaacgacgc ctgcgcaccc tcatcgaaca atatcaactg gaagatgtga tagagatgcc
    21961 gggctttaaa ccgagccatg aagtgaaggc gatgctcgac gacgcggatg tcttcctgtt
    22021 gccatcggtt acaggtgcgg acggcgatat ggaaggtatt ccggtggcac taatggaagc
    22081 gatggcggtc ggcattccgg tggtttctac tctgcatagc ggaataccgg aactggtgga
    22141 ggccgataaa tccggctggc tggtgcctga gaacgatgct cgcgcactgg cgcaacgact
    22201 ggcggcgttt agccaactgg acaccgacga actggctccg gttatcaaac gtgctcgcga
    22261 aaaagtcgaa cacgatttta accagcaggt gatcaatcga gaactcgcca gcttgctgca
    22321 ggctttatag aggagaacgt atgccattta aaaaactctc ccgacgcacc ttcctgacgg
    22381 caagctcggc gcttgccttc ctccataccc ccttcgcccg cgcacttcca gcccgacaaa
    22441 gcgttaatat caacgattac aatccgcacg actggatcgc ctcatttaaa caagccttca
    22501 gtgaagggca aacggtcgtc gtgcctgccg gattggtttg tgacaatatc aacaccggca
    22561 tcttcattcc tcctggaaaa accttacaca tccttggaag cctgcgcggc aacggcagag
    22621 ggcgatttgt cttacaggac ggcagccagg tgacagggga ggagggcggc agtatgcata
    22681 acatcaccct ggatgtgcgc ggttctgact gcatcatcaa agggttggcg atgagcggtt
    22741 ttggcccggt aacgcagatt tatatcggcg gcaaaaataa acgcgtcatg cgcaacttga
    22801 ccatcgataa cctgaccgtc agccacgcta attacgccat tttacgccag ggatttcata
    22861 accagattat cggtgccaac atcaccaact gtaagttcag cgacttacag ggcgacgcca
    22921 ttgaatggaa tgtggcgatt aacgaccgcg atattttgat atccgaccac gttattgagc
    22981 gcatcaactg taccaacggc aaaatcaact ggggcatcgg cataggcctt gcgggaagca
    23041 cttacgataa caactatccg gaagaccagg cagtgaaaaa ctttgtcgtg gcgaatatca
    23101 cgggatcaga ttgtcgacag ttgatacatg ttgaaaatgg taaacatttt gttattcgta
    23161 atatcaaagc ccgcaatatc acgccggatt tcagtaagaa agcaggtatt gataacgcca
    23221 cggtcgctat ttacggttgt gacaatttcg tgattgataa tattgaaatg attaatagcg
    23281 ccgggatgtt aattggctat ggggtaatta aaggcaaata tctctcgata ccgcaaaatt
    23341 tccgagtgaa taatattcaa ctggataaca ctcaccttgc ttataaattg cgcggcatcc
    23401 aaatctccgc cgggaatgct gtctcctttg tggcgctgac taacattgag atgaagcgtg
    23461 cctcgctgga gcttcacaat aaaccgcaac atctttttat gcgaaatatc aaagtgatgc
    23521 aggaatcctc tgttggaccc gcattgagca tgaacttcga catgcgcaaa gacgttcgcg
    23581 gcgtctttat ggcgaaaaaa gaaacactgc tgtctcttgc aaatgttcat gcgatgaatg
    23641 aaagagggca aagctccgtc gatatcgaca gggttaatca ccatattgtt aatgtggaaa
    23701 agattaactt tagattgcca gaacggaggg agtagatttg cgaccattca taaacttact
    23761 ggatataatg ttctaaaaga ttataatcct cacactaact gttaaagctg gggctgggca
    23821 gcattgacta ctcagggaag gtactcatct ttatcttgtg tttcttacgt aacaaccaga
    23881 ctaaagtgat ttatgaacga taacgttttg ctcataggag cttccggatt cgtaggaacc
    23941 cgactacttg aaacggcaat tgctgacttt aatatcaaga acctggacaa acagcagagc
    24001 cacttttatc cagaaatcac acagattggc gatgttcgtg atcaacaggc actcgaccag
    24061 gcgttagctg gttttgacac tgttgtgcta ctggcagcgg aacaccgcga tgacgtcagc
    24121 cctacttctc tctattatga tgtcaacgtt cagggtaccc gcaatgtgct gacggccatg
    24181 gaaaaaaatg gcgttaaaaa tatcatcttt accagttccg ttgctgttta tggtttgaac
    24241 aaacacaacc ctgacgaaaa ccatccacac gatcctttca accactacgg caaaagtaag
    24301 tggcaggcag aggaagtgct gcgtgaatgg tataacaaag caccaacaga acgttcatta
    24361 accatcatcc gtcctaccgt tatcttcggt gaacgcaacc gcggtaacgt ctataacttg
    24421 ctgaaacaga tcgctggcgg caagtttatg atggtgggcg cagggactaa ctataagtcc
    24481 atggcttatg ttggaaacat tgttgagttt atcaagtaca aactgaagaa tgttgccgca
    24541 ggttatgagg tttataatta cgttgataag ccagatctga acatgaacca gttggttgct
    24601 gaagttgaac aaagtctgaa caaaaagatc ccttctatgc acttgcctta cccactagga
    24661 atgttgggtg gatattgctt tgatatcctg agcaaaatta cgggcaaaaa atacgctgtc
    24721 agctctgtgc gcgtgaaaaa attctgcgca acaacacagt ttgacgcaac gaaagtgcat
    24781 tcttcaggtt ttgtggcacc gtatacgctg tcgcaaggtc tggatcgaac actgcagtat
    24841 gaattcgttc atgccaaaaa agacgacata acgtttgttt ctgagtaatg cagacgagtc
    24901 agagaaaaaa ggaccaggtt ggactacgat tattcggaaa gaggtactga ttaaattatc
    24961 agtatgcgga attccgagta atccgagtaa cccagatgct gatggtgtgt agtgttattc
    25021 taaggtttaa gcaaagttat gcggaactaa gctttggtat atagccataa cgtttggcta
    25081 acctatggca ataatttacg cactatcagc cacttagagg taggaaaaga atgacgaatt
    25141 taaaagcagt tataccggta gcaggtcttg ggatgcatat gttgcctgcc actaaggcga
    25201 ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    25261 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    25321 aaaaccactt cgacacctct tatgaattag aatctctcct tgagcagcgc gtgaagcgtc
    25381 aactactggc ggaagtgcaa tctatctgcc cgccgggcgt gactattatg aacgtgcgtc
    25441 agggcgaacc tttaggttta ggtcactcca ttttgtgtgc acgccccgcc attggtgaca
    25501 acccatttgt cgtggtgctg ccggacgttg ttatcgatga tgccagcgcc gacccgctgc
    25561 gttacaacct tgccgctatg attgcgcgct tcaacgaaac gggccgcagc caggtgctgg
    25621 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagaccaaa gaaccgctgg
    25681 atcgtgaagg caaagtcagc cgcattgttg aattcatcga aaaaccggat cagccgcaga
    25741 cgctggactc agacatcatg gccgttggtc gctatgtgct ttctgccgat atttggccgg
    25801 aactggaacg cactcagcct ggtgcatggg ggcgtattca gctgactgat gctattgccg
    25861 aactggcgaa aaaacagtcc gttgatgcca tgctgatgac tggtgacagc tacgactgtg
    25921 gtaaaaaaat gggctatatg caggcgtttg tgaagtatgg actgcgcaac ctgaaagaag
    25981 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    26041 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcgaaa gtaatttgtt
    26101 gcgaatattc ctgccgttgt tttatataaa caatcagaat aacaaagagt tagcaatagg
    26161 attttcgtca aagttttcca ggattttcct tgtttccaga gcggattggt aagacaatta
    26221 gcgtttgaat ttttcgagtt aagcgcgagt gggtaacgct cgtcacatcg taggcatgca
    26281 tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt gcattaatac ctctattaat
    26341 caaactaaga gccgcttatt tcacagcatg ctctgaagta atatggaata aaagagtgaa
    26401 gatacttgtt actggtggcg caggatttat tggttctgct gtagttcgtc acattataaa
    26461 taatacgcag gatagtgttg ttaatgtcga taaattaacg tacgccggaa acctggaatc
    26521 acttgctgat gtttctgatt ctgaacgcta tatttttgaa catgcggata tttgcgatgc
    26581 tgctgcaatg gcgcggattt ttgctcagca tcagccggat gcagtgatgc acctggctgc
    26641 tgaaagccat gtggatcgtt caattacagg ccctgcggca tttattgaaa ccaatattgt
    26701 tggtacttat gtccttttgg aagccgctcg caattattgg tctgctcttg atggcgacaa
    26761 gaaaaatagc ttccgttttc atcatatttc tactgacgaa gtctatggtg atttgcctca
    26821 tcctgacgaa gtaaataata aagaacaatt acccctcttt actgagacga cagcttacgc
    26881 gcctagtagt ccttattccg catcaaaagc atccagcgat catttagtcc gtgcgtggaa
    26941 acgtacctat ggtttaccga ccattgtgac taattgttcg aataactacg gtccttatca
    27001 ctttccggaa aaattgattc cactagtaat tcttaatgct ctggaaggta aggcattacc
    27061 tatttatggc aaaggggatc aaattcgcga ctggctgtat gttgaagatc atgcacgtgc
    27121 gttatatacc gtcgtaaccg aaggtaaagc gggtgaaact tataacattg gtggacacaa
    27181 cgaaaagaaa aacatcgatg tagtgctcac tatttgtgat ttgttggatg agattgtacc
    27241 gaaagagaaa tcttaccgcg agcaaattac ttatgttgcc gatcgtccgg gacacgatcg
    27301 ccgttatgcc attgatgctg agaagattgg tcgcgaattg ggatggaaac cacaggaaac
    27361 gtttgagagc gggattcgga agacagtgga atggtacctg tccaatacaa aatgggttga
    27421 taatgtgaaa agtggtgcct atcaatcgtg gattgaacag aactatgagg gccgccagta
    27481 atgaatattc tccttttcgg caaaacaggg caggtaggtt gggaactaca gcgtgctctg
    27541 gcacctttgg gtaatttgat tgctcttgat gttcactcca ctgattattg tggtgatttt
    27601 agtaatcctg aaggtgtggc tgaaaccgtc aaaaaaattc gccctgatgt tattgttaat
    27661 gctgcggctc ataccgcagt agataaggct gagtcagaac ccgaatttgc acaattactc
    27721 aatgcgacca gcgttgaatc aattgcaaaa gcggctaatg aagttggggc ctgggtaatt
    27781 cattactcaa ctgactacgt atttccggga accggtgaaa taccatggct ggagacggat
    27841 gcaaccgcac cgctaaatgt ttacggtgaa accaagttag ctggagaaaa agcattacaa
    27901 gagcagtgtg cgaagcacct tattttccgt accagctggg tatatgcagg taaaggaaac
    27961 aattttgcca agacaatgtt acgtctggca aaagagcgtg aagaattagc cgttattaat
    28021 gatcagtttg gtgcgccaac tggcgcagag ttgctggctg attgtacggc acatgccatt
    28081 cgtgtggcac tgaataaacc ggaagtcgca ggtttgtacc atctggtagc cagtggtacc
    28141 acaacctggc acgattatgc tgcgctggtt tttgaagagg cgcgcaaagc aggcataccc
    28201 cttgcactca acaagctcaa cgcagtagca acaacagcct atcctacacc agctcgtcgt
    28261 ccacataact ctcgccttaa tacagaaaaa tttcagcaga attttgcgct tgttttgcct
    28321 gactggcagg ttggcgtgaa acgaatgctc aacgaattat ttacgactac agtaatttaa
    28381 tagtttttgc atcttgttcg tgatgatgga gcaagatgaa ttaaaaggaa tgatgaaatg
    28441 aaaacgcgta aaggtattat tttagcgggt ggctctggta ctcgtcttta tcctgtgact
    28501 atggctgtca gtaaacagct gttacctatt tatgataaac cgatgatcta ttacccgctt
    28561 tctacactga tgttagcggg tattcgcgat attctgatta tcagtacacc acaggatact
    28621 cctcgttttc accaactgct gggtgacggg ggccagtggg ggctaaatct tcagtacaaa
    28681 gtgcaaccga ctccagatgg gcttgcgcag gcgtttatta tcggtgaaga gtttatcggt
    28741 ggtgatgatt gtgctctggt tctcggtgat aatatctttt acggtcatga tctgccgaag
    28801 ttaatggatg tcgctgttaa caaagaaagt ggtgcaacag tatttgccta tcatgttaat
    28861 gatcctgaac gctacggtgt cgttgagttt gataaaaaag gtacggcaat tagcctggaa
    28921 gaaaaaccgt tacaaccaaa aagtaattat gcggtaaccg ggctttattt ctatgataac
    28981 gacgttgtcg aaatggcgaa aaaccttaag ccttctgccc gtggtgaact ggaaattacc
    29041 gatattaacc gtatttatat ggaacagggg gggttatccg ttgccatgat gggacgtggt
    29101 tatgcatggc tggacacggg tacacatcaa agtcttattg aagcaagcaa cttcattgca
    29161 acaattgaag agcgtcaggg gttaaaagtt tcctgtccgg aagaaattgc ttaccgtaaa
    29221 gggtttattg atgctgagca ggtgaaagta ttagctgaac cgctgaaaaa aaatgctta
//
LOCUS       DAZUCC010000039        28708 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZUCC010000039 DAZUCC010000000
VERSION     DAZUCC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.039"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..9737)
                     /gene="cdiA"
                     /locus_tag="ACHY09_001540"
     CDS             complement(<1..9737)
                     /gene="cdiA"
                     /locus_tag="ACHY09_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition toxin CdiA"
                     /protein_id="HFS3884528.1"
                     /translation="MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GKPVMNADGSLQALEVTEGSITINGAGLDGTWSDAVSIIARATEVNAALHAKDLTVTA
                     GANRITADGRVSALKGEGNVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARE
                     GDIILSSSGKLVLKNSLAGGSTTVTGTDVSLSGDNKAGGNLSVTGTTGLTLNQSRLVT
                     DKNLVLSSSGQIVQNGGELTAGQNAMLSALHLNQTSGAVNAAENVTLTTTDDTTLKGR
                     SVAGKTLTVSSGSLNNGGTLVAGRDATVKTGTFSNTGAVQGNGLKVTATDLTSTGSIK
                     SGSTLDISARNATLSGDAGAKDSARVTVSGTLENRGRLISDDVLTLSATQINNSGTLS
                     GAKELVASADTLTTTEKSVTNSDGNLMLNSASSTLAGETSAGGTVSVKGNSLKTTTTA
                     QTQGNSVSVDVQNAQLDGTQAARDILTLNASEKLTHSGKSSAPSLSLSAPELTSSGVL
                     VASALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSGLI
                     TGDNGLTLNTASLSNPGKITADTLNVRATTLDGDGLLQGAAALALAGDTLSQGRHGRW
                     LTAGDLSLRGKTLNTAGTTQGQNLTVQADRWANRGSVLATGNLTASATGQLTSTGDIM
                     SQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTLHHRSTDNSGTVTGL
                     SGLTLHSADGLTNSGALLSQNSLVLSAGDVTNSGRIQGQNITLDASSLTSSGAVQSAL
                     DLALTLSGDVIAATGSKITALGDARLSGKVLGNQGLISAKTLEVNGDSLSNSGEISGV
                     NSLNVTLSGNLQQHGKMLTGGALNVNARDISNSGQLQGADNRITASSLANSGRVQGES
                     GLTLTLLNALTNQTSGVLLSQNVSALSAPALTNDGTIQGNGKTTLSAATQAHNSGKIL
                     SGGELTFTTPDYSGSGWLQATDLLLNVAKLAGNGTVMAANQATLTGNSLTNRGLFQAA
                     QLNVNTQTITNSGTLLGNQGLTIKGNNLNNAGGKVFSGGDMLAEMVSLSGAGQLVALG
                     NLTLKLTRGLTAQGVIAANKQLSVSSQGDITNGATLQGNGITLNAAGRLTNNGQLTAG
                     NGTTALSGSGIAMNASGSLQAGGDVSLTSRGDITLDAFTGTTGSLMLTAAGAVINTAL
                     LYAGNNLSLFASTIRNHHGDMLAGDSLVMQKDVSGAANAEVINTSGNIETTRGDITIR
                     TGHLLNQREGINETKSYIPVENVAVPDGANSVSVRVGDLGEDGWGYYVKSWSGTAGGG
                     FDAWAVPTEKGATRKFLTGTTRVDVGATGGDARISAGNNLLIDADKLDNTGSHLLASG
                     FVSLSGSQLNNQSFFGYTQDEYNVYRYYGKLAMIPNDGHLQYGDASADDRVTFTLSGA
                     PEYVTRDTGQALRAVIQAGKNVTAVFSSDISNTSTTSNAGRITNTLAAPEINTPAEKN
                     ISPGMAQLAPDGTETLTVTAPDWTDTITRLTIGSGTDLASGIVEGNYPLPSGNNGYFV
                     PSTDPDSPYLITVNPKLDGLGKVDSSLFTGLYDLLRMHPGQAPRETDPAYTDEKQFPG
                     SSYFLDRLGLKPEKDYRFLGDAAFDTRYVSNYMLNQIGGRYINGVGSDTDQMRYLMDN
                     AARAQKALGLKFGVALTADQVAALDQSILWYKAVTIKGQTVMVPEVYLSPKDVTLQNG
                     SIISGQNVHLAGGNVTNSGSTLMAQNNLTIDSADSLGNLESGLINAGGALGLKAMGDI
                     NNISATITGKTVRLESLAGNVNNLTRYSHWQLDAPEDSLALKHTYTGSIASVSAMDSL
                     DIRADKNISVTGAEISAGDRAALIAGNDLSLNAIDRVSSRRHANSESHQRSAGLTTIT
                     AGDSVMLSAGRDVSSQGAGIAAEDNITVRAGRDVNLLAEESVTGSSSYSKKKTVIDET
                     VRQQGTEIASGGDTTITAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLET
                     KKKSGGFLSKKTTHTISEDSATREAGSLLSGNRVTVNAGDNLTVEGSDVVADRDVSLA
                     AGNHVDVLAVTSTDTSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSAS
                     TIGSTAGNVSITAGKQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSG
                     LTVALSGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGD
                     PNATGVSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGS
                     QLKAGGNTSLDAANDILLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVN
                     AAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQ
                     DTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDI
                     TVGRHTQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDK
                     FQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNPADLSRDPAHANDSI
                     SPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQ
                     RQEYLAKLRDTPEYKKEQEKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPEL
                     AHLLKSTEKDPVVNAIAHAILGGAVAAMQGNNVAAGAAGAATGEMAARAIAGMLYPGV
                     KQSDLSEEQKQTISTLATVSAGLAGGLTGNSSASAAVGAQSGKNAVDNNYLSVSEKTE
                     LEIAKQTLKNSKNPAEREKAQQKYDALLEKDIASDKEVIAACGNGNAGSSACASARLK
                     VIASKEGYEDGPYNSKYSQQYADAYGQIVNLLDITSVDVQNQQQVKDAMVSYFMATLG
                     VDQKTAQGYVETTQGLEIAAASMTPLFGQAVANKITALVDKANKYPSGIGFKINQPEH
                     LAQLDGYSQKKGISGAHNADVFNKAVVDNGVKIISETPTGVRGITQVQYEIPTKDAAG
                     NTTGNYKGNGAKPFEKTIYDPKIFTDEKMLQLGQEAAAIGYSNAIKNGLQAYDAKAGG
                     VTFRVYIDQKTG"
     gene            complement(9750..11528)
                     /locus_tag="ACHY09_001541"
     CDS             complement(9750..11528)
                     /locus_tag="ACHY09_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFS3884529.1"
                     /translation="MQHRQNNILTKTSLLFRAVSVPCYDIFRRARASSGICYLSLSVF
                     SGFFIPAFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTVIPSPEMSAG
                     TEGPCFTVSRIVVSGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYIRRGYI
                     TSRAFLTEQDLSGGVLHITVMEGRLQQIRAEGADLPGRTLKMVFPGMEGKVLNLRDIE
                     QGMEQINRLRTEPVQIEISPGDREGWSVVTLTASPEWPVTGSVGIDNSGQKNTGTGQL
                     NGVLSFNNPLGLADNWFVSGGRSSDFSVSHDARNFAAGVSLPYGYTLVDYTYSWSDYL
                     STIDNRGWRWRSTGDLQTHRLGLSHVLFRNGDMKTALTGGLQHRIIHNYLDDVLLQGS
                     SRKLTSFSVGLNHTHKFLGGVGTLNPVFTRGMPWFGAESDHGKRGDLPVNQFRKWSLS
                     ASFQRPVTDRVWWLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKEQYISGNNGGYLRN
                     ELSWSLFSLPYVGTVRAVTALDGGWLHSDRDDLYSSGTLWGAAAGLSTTSGHVSGSFT
                     AGLPLVYPDWLAPDHLTVYWRVAVAF"
     gene            12028..12417
                     /locus_tag="ACHY09_001542"
     CDS             12028..12417
                     /locus_tag="ACHY09_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884530.1"
                     /translation="MKRYIKWLAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKN
                     AILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDNSLNLQASDG
                     KIHKNYNRWVRNLDKDIQVNLSTGATL"
     gene            12481..13539
                     /locus_tag="ACHY09_001543"
     CDS             12481..13539
                     /locus_tag="ACHY09_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFS3884531.1"
                     /translation="MYEQDTLSTLDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEQTVLNEYAVSVLLDMGLSGRIITCKEGNYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARASWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFQAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            13580..14302
                     /locus_tag="ACHY09_001544"
     CDS             13580..14302
                     /locus_tag="ACHY09_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFS3884532.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRYQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALMIESGDDWQCETQSAIAVNETTLPQSMLFLQH
                     YLQNAGAFSLSGERVQWRWSRR"
     gene            14299..15120
                     /locus_tag="ACHY09_001545"
     CDS             14299..15120
                     /locus_tag="ACHY09_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS3884533.1"
                     /translation="MNKLMSRLGWAWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVHGRVEINKFYDATSHEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            15095..15352
                     /locus_tag="ACHY09_001546"
     CDS             15095..15352
                     /locus_tag="ACHY09_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFS3884534.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTNAPLFGDGLGLDSIDA
                     LELGLAVKNEYGVVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            15364..15615
                     /locus_tag="ACHY09_001547"
     CDS             15364..15615
                     /locus_tag="ACHY09_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS3884535.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVKRLLQEG"
     gene            15620..16201
                     /locus_tag="ACHY09_001548"
     CDS             15620..16201
                     /locus_tag="ACHY09_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884536.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFLIWLGLAHNSLHWLSPLMALL
                     LLLRLRQPRRQAGPLQAVTKIVAVVGIVLCVSSFLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRCQMIKRKTP"
     gene            16198..17556
                     /locus_tag="ACHY09_001549"
     CDS             16198..17556
                     /locus_tag="ACHY09_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFS3884537.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCILSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNNMNKRVYAQLQELFHD
                     TP"
     gene            17543..17896
                     /locus_tag="ACHY09_001550"
     CDS             17543..17896
                     /locus_tag="ACHY09_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFS3884538.1"
                     /translation="MIRHEIERHQVQLQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            17887..19563
                     /locus_tag="ACHY09_001551"
     CDS             17887..19563
                     /locus_tag="ACHY09_001551"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS3884539.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENTGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTCYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQGLQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            19567..19989
                     /locus_tag="ACHY09_001552"
     CDS             19567..19989
                     /locus_tag="ACHY09_001552"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS3884540.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            19986..20591
                     /locus_tag="ACHY09_001553"
     CDS             19986..20591
                     /locus_tag="ACHY09_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFS3884541.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLSDDERQRFAAQ"
     gene            20560..22878
                     /locus_tag="ACHY09_001554"
     CDS             20560..22878
                     /locus_tag="ACHY09_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFS3884542.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVVLLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILMIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLGRLETFTTSLEKA
                     KKDGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALQQEIASYYPGGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            22875..23459
                     /locus_tag="ACHY09_001555"
     CDS             22875..23459
                     /locus_tag="ACHY09_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFS3884543.1"
                     /translation="MIKSTFWRALALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLSGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAKG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPTGWTLRDNGNKRELRNASGKL
                     VTEIIYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            23461..24630
                     /locus_tag="ACHY09_001556"
     CDS             23461..24630
                     /locus_tag="ACHY09_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFS3884544.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTNGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDIG
                     YINLHGTATQLNDQIESMVVNALFGKRIPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            24627..25091
                     /locus_tag="ACHY09_001557"
     CDS             24627..25091
                     /locus_tag="ACHY09_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFS3884545.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            25091..25822
                     /locus_tag="ACHY09_001558"
     CDS             25091..25822
                     /locus_tag="ACHY09_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFS3884546.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            25819..27048
                     /locus_tag="ACHY09_001559"
     CDS             25819..27048
                     /locus_tag="ACHY09_001559"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFS3884547.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARSATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(27222..27491)
                     /locus_tag="ACHY09_001560"
                     /pseudo
     CDS             complement(27222..27491)
                     /locus_tag="ACHY09_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27888..>28708)
                     /locus_tag="ACHY09_001561"
     CDS             complement(27888..>28708)
                     /locus_tag="ACHY09_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS3884548.1"
                     /translation="TVAVLVPSLTDKASSRFMQSLQQVLNKNEFQLLLGCHEYNQLKE
                     AEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPFSPAQITIETAFFE
                     ASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQNTDLVITMPDAAS
                     LQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDNC
                     DQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
BASE COUNT         6280 a         7941 c         7493 g         6994 t
ORIGIN      
        1 cctgtttttt gatcaatata tactctgaac gtaacaccac cggcttttgc atcatatgcc
       61 tgtaatccat tttttattgc attggaataa ccaattgccg ctgcctcttg accaagttgc
      121 aacattttct catctgtaaa tattttagga tcataaatag ttttctcaaa aggcttggcg
      181 ccatttcctt tataattacc agtcgtattc ccagcagcat cttttgtggg gatttcatac
      241 tgaacctgag taatacctct gactcctgtt ggtgtttcag aaataatttt cacaccatta
      301 tcaaccacag ctttattgaa tacatcagca ttatgtgcac cgctaattcc tttcttctgc
      361 gaatatccgt caagttgagc taagtgttcc ggctgattaa ttttaaaacc aatccctgaa
      421 ggatatttat tcgctttatc aaccaatgca gtaattttat tagccacagc ctgaccaaat
      481 aatggtgtca ttgaagccgc tgcaatttct aatccctgcg tcgtctcgac ataaccttgt
      541 gctgttttct ggtcaacccc cagcgttgcc atgaagtaac tcaccatcgc atctttcacc
      601 tgctgttgat tttgtacatc aacactggtg atatcgagga gattgactat ctgaccatac
      661 gcatcagcgt attgctggct gtatttcgag ttataagggc catcttcgta cccttctttc
      721 gacgcaataa ctttcagtct tgcgctggca cacgctgaac tacccgcatt accattaccg
      781 caggcagcaa taacctcctt atcactggca atatcttttt caagcaatgc atcatatttc
      841 tgctgtgcct tttcacgctc tgccggattt ttactgtttt taagcgtctg ttttgctatc
      901 tcaagctctg tcttttcaga cacgctcagg tagttattat caacagcatt cttcccgctc
      961 tgtgcgccca ctgctgctga tgcgctgctg tttccggtaa gaccgccagc cagccctgct
     1021 gacacggttg ccagcgtact tatcgtctgc ttctgctctt cactgaggtc tgactgtttc
     1081 acgcccggat acagcatgcc ggcaatcgct ctggctgcca tctctcccgt tgctgcgcct
     1141 gctgcaccgg cagcaacatt attaccctgc attgccgcta ctgcgccacc cagaatggcg
     1201 tgagcaatgg catttactac cggatctttt tctgttgatt tcagcaggtg cgccagttcc
     1261 ggggccgaag ctccggccag agcacccgct aaattccccc cagccagtcc ctgaagcgcg
     1321 gctgtcgcgg cctgtatacc ccgctgaatc tcactgccag tgccatactt ttcctgttcc
     1381 tttttgtatt ccggcgtatc acgcagtttc gcaagatatt cctgacgttg tttctctgtt
     1441 gcattagcag gaagtgtttc gccggttgct tccttcgcgg ccttcaacgc attcagttcc
     1501 ccctgcgtcc gcgcgatatc cgccacctga ctgcctatgt cactgataag ccccactgtc
     1561 tgcagacgcc tctgctcctt ctccttgtca aatatcgggc tgatactgtc attagcgtgc
     1621 gcaggatcac ggctgaggtc tgccggattc tgcttctgat tgtccctgtc ccggatggtg
     1681 atagtgcctt ctgccactgc ggcctgagtc gttccttccg catgtccgct gtgacctccg
     1741 gcggatatca tgccacccgg catgttaccc tgaaatttat ccccgaagct gccaccaccg
     1801 ctcagactga tcccactgtg actgacttta taatccgctt cgttgtgaag gtcactgaac
     1861 cccagcgttc cggtatccag gtggttttta tccggtgtgg cagtggaggc aatcaccgca
     1921 ccatccagtt gggtatgtct gcccactgtg atgtcgaagc caccatcacc ggcaaacatt
     1981 ccggtttgtt cagcaacgga gtcaaagcgg ctcttcatct tatcccggga ggcagcgatg
     2041 taacctgagc cggtcatgga gccaaaggta aaactgccgc cggcagccac gctggtctgt
     2101 ttactgtcgt acttactggt gtcctgctgg ctgctaatca gcaggtcgtg gcccacatcg
     2161 gcgataatcc tgttgccgtt gacctgagca ccgttcagta ccgtatcccg accactgttg
     2221 atggtgacgg ttttaccgct gtctgttgtg gtttcagtcc actcagtacc gttacctttc
     2281 tcgctgcctt ttgccgcatt aacgctggca aagacgctga taccggcacc gttaccaccg
     2341 gcaccgatac tgacacccac gccaccgcca ctgctgctgt tcctgcccgt tgttttttgt
     2401 gtgtttgcag cgccactcaa cagaatatca ttcgcggcat ccaggcttgt gttaccaccg
     2461 gccttaagct ggcttcctgc aatcacaata tctccgcggt tatcgcccct gtttttgccg
     2521 gttgcgacaa cagacagatt attcccggca ttcagcgtac tgccggatac tgtgtcactt
     2581 tcagaatgtt gttgtgattt cgatttctga gtggtgagcg acaggctgac tcccgtcgca
     2641 ttcgggtcac cggttgcggt ggccattgcc gcagcctgtc cggcctgcac accagacagc
     2701 gctgtctttg tggcctgcag ggctttcaga cgactgtcac tgctctcctt cgtttcctgt
     2761 gcactggtga ccgcattatt gatggcactg cccactgtgc cggaaagggc aaccgtcagc
     2821 ccgcttttct tctgctcaaa tttttcgtcc acagtacgac ggtcatgccc cgggtcaacc
     2881 accacactgt caccggtaat gctgatatcc cggttcgcaa tcacatccga accgctgata
     2941 tgagcctgtt tgcccgcggt aatactgaca ttaccggcag tggagccgat ggtactggca
     3001 ctctgactct gcgttgtccc ggcctcgcgg cggtcgtgcg ttgtcttact gctgccgatg
     3061 gtgaagccga taccgccagt acccatcaga cccgatttct tcgtttcctt aaagcgccag
     3121 gacgtatctg tactggtgac agcaagaaca tcaacatggt tacccgccgc cagtgacaca
     3181 tcccggtcag ccaccacatc cgaaccctct accgtcaggt tatcaccggc gttaacggtc
     3241 acgcggttcc ccgacagcag ggagcctgct tcacgggtgg cactgtcctc actgatggtg
     3301 tgggtggttt tcttactgag aaaacctccg ctttttttct tcgtttccag atagtgatag
     3361 tcactttctg tcgccgtggt caaggcaaca tcacgaccgg cattcacgct gatattgccg
     3421 gttgcggtaa cggatgacgc aacagcggtg atatcccgtc ctgcagtaat ggtggtgtca
     3481 ccaccgctgg cgatttccgt tccctgctgg cgaacggttt catcaatgac cgttttcttt
     3541 ttcgagtatg aactgctgcc ggtcacggac tcttccgcca gcagattaac gtcgcgcccg
     3601 gcccggacag tgatgttgtc ttccgctgcg atacctgcac cctgactgct cacatcccgg
     3661 cctgcactca gcatcacact gtcgccggca gtaatggtcg taagcccggc gctgcgctgg
     3721 tgagactcac tgtttgcatg acgtctgctg cttaccctgt caatggcatt cagactgaga
     3781 tcattgccgg caataagtgc tgcgcggtct ccggcactga tttctgcccc tgtgaccgaa
     3841 atgtttttat ctgcccggat gtccagacta tccatggcac tgacagaggc aatgcttccc
     3901 gtataagtgt gtttcagggc gaggctgtct tccggtgcat caagctgcca gtgtgagtac
     3961 cgggtgagat tattgacatt gccggcgaga ctctccagcc ggacagtctt accggtaatc
     4021 gtggcactga tattgttgat atcgcccatc gctttcagtc cgagagcgcc accggcattt
     4081 ataagcccgg actcaaggtt acccagactg tcagcgctgt cgatggtgag attattctgc
     4141 gccatcaggg tactgccact gttagtgaca ttccctcctg ccaggtgcac attctgaccg
     4201 ctgataatac tgccgttctg cagcgtcaca tctttcggtg acagatacac ctccggcacc
     4261 atcaccgtct gaccctttat cgtgacggct ttataccaca gaatactttg gtcgagagcc
     4321 gccacctggt ctgctgtcag tgccacacca aacttcagac cgagcgcctt ctgtgcacgg
     4381 gcagcgttat ccatcaggta gcgcatctgg tcggtgtctg aaccaacccc gttgatatag
     4441 cgccccccga tctggttcag catatagttc gatacatagc gggtatcaaa agcggcatcc
     4501 cccaggaagc gataatcttt ttccggtttc agcccgagcc ggtcaagaaa atacgatgag
     4561 cccgggaatt gtttttcatc agtatacgcc gggtctgttt cacgtggtgc ctgcccgggg
     4621 tgcatcctga gcaggtcata cagtccggta aacagactgc tgtccacctt tccgagacca
     4681 tccagtttcg gattcaccgt aatcagatat ggactgtcag ggtccgtgga cgggacaaaa
     4741 taaccattgt taccggaagg caggggatag ttcccctcca cgatgccgga agccaggtcc
     4801 gtgccactgc cgatcgtcag acgggtaatg gtgtcagtcc agtccggggc tgtgacggtc
     4861 agcgtctctg tgccatccgg cgccagttga gccattcccg ggctgatatt tttctctgcc
     4921 ggcgtgttaa tttcaggtgc cgcaagggta tttgtgatcc gtcctgcatt tgaggtggtg
     4981 gatgtattac tgatatcact ggaaaaaacg gcagtgacat ttttgcctgc ctgaatcacg
     5041 gctctcagag cctgaccggt gtctctggtg acatattccg gagctccgga gagagtaaag
     5101 gtaacgcgat catcggcaga agcatctcca tactgcagat gcccgtcgtt ggggatcatg
     5161 gccagtttgc cgtaatagcg atacacattg tattcatcct gtgtatagcc aaagaaggac
     5221 tgattattca gttgtgaacc cgacaggctg acaaacccgc tggcaagaag atgacttccc
     5281 gtattatcca gtttgtccgc gtcaatcagc agattattcc cggccgaaat tctggcatcc
     5341 ccccctgttg caccaacatc gacgcgtgtt gtgccagtca gaaatttccg ggtggctccc
     5401 ttttcagtcg gaacggccca tgcgtcaaaa ccaccaccgg cagtaccgct ccaggacttc
     5461 acataatatc cccatccatc ttcacctaaa tcgcccactc tgactgaaac agaatttgca
     5521 ccatcaggta ctgcgacatt ctcaaccgga atgtatgatt ttgtctcatt tatgccctcc
     5581 cgctgattca gtaaatgtcc ggttctgatg gtgatatcac cgcgggtggt ttcgatattc
     5641 ccggaggtat taatcacttc agcattcgcg gcaccactga catctttctg catcaccagg
     5701 ctgtcacccg ccagcatatc gccgtgatgg ttccggatgg tgctggcaaa cagcgagagg
     5761 ttattgcccg catacaacag tgcggtgtta atgactgccc cggcggccgt taacatcaga
     5821 ctacccgtcg tgccggtaaa agcgtcgaga gtgatatctc ccctgctggt caggctgacg
     5881 tccccgcctg cctgcaggct gccgctggca ttcatcgcaa tcccgctgcc gctcagtgcc
     5941 gtggtgccgt tgccggcggt cagctggcca ttgttggtca gtctgcctgc agcattcagc
     6001 gtgataccgt tgccctgcaa tgtggcgcca ttggtaatat ccccctggct gctgacggag
     6061 agttgtttat ttgcggcgat aacgccctgt gcggtcaaac cacgggtgag cttcagcgtc
     6121 aggttaccga gtgcaaccag ctggcccgcg ccgctgagcg aaaccatttc agcaagcata
     6181 tcaccgccgc tgaacacctt cccgccggcg ttgttcaggt tgttcccttt tattgtcagg
     6241 ccctgattac ccagcagcgt gccgctgttg gtgatggtct gcgtgttgac gttcagttgt
     6301 gcggcctgga acagaccacg gttggtcaga ctgttacctg tgagcgttgc ctgattcgcc
     6361 gccatcacag tgccattacc ggcaagcttc gccacgttta gcagcaggtc agtggcctgt
     6421 agccagccac tgccactgta gtccggcgtg gtgaacgtca gctcaccgcc ggaaagaatc
     6481 ttgccactgt tgtgggcctg cgttgccgcg ctgagcgtgg ttttaccgtt gccctgaatg
     6541 gtgccgtcat tggtcaacgc gggtgcggaa agcgctgata cgttctggct gagaagcact
     6601 ccgctggtct ggttggtcag ggcattcagc aatgtaagag tcagcccgct ctcgccctga
     6661 acacgacctg agttggccag cgaactggcg gtgattcggt tatccgcccc ctgaagctgc
     6721 ccactattgc tgatgtctct ggcgttgacg tttaatgcac cgcccgtcag cattttaccg
     6781 tgctgttgca gattaccact gagtgtcacg ttcaggctgt taaccccact gatttcgcca
     6841 ctgttgctga gcgaatcgcc attcacttcc agcgtcttcg cgctgattaa tccctggtta
     6901 cccagcactt tgccgctcag acgggcatcg cccagggcgg taattttgct gccggtggcg
     6961 gcaataacat cgccactcag ggtcagagcc aaatccagcg cactctgaac agcaccgctg
     7021 ctggtcaggc tgctggcatc cagagtgatg ttctgcccct gaatccggcc gctgttggtc
     7081 acatctcctg cgctcagcac cagcgagttc tggctcagca gcgcgccact gttggtaagc
     7141 ccgtccgcgc tgtgcagggt cagaccgctc agccccgtga ccgtaccgct gttgtcagtg
     7201 cttctgtggt gaagcgtgac atggttaccc tggacagtgc cgctgttatc cagtgagttt
     7261 ccatcaaggg agagcgtgcc ggccgaaagc agactgcccc ggttgtccgt ggtggctgcg
     7321 ttcagcgtgg tgtcaccctg gctcatgata tcgccggtac tggtcaactg accggttgcc
     7381 gaagcagtaa ggttaccggt tgccagcacg gaaccacggt tcgcccatct gtccgcctgc
     7441 acggtgagat tctgtccctg cgtggtccct gcggtattca gtgttttacc ccggagggag
     7501 aggtcgcccg ccgtcagcca gcgtccgtga cgaccctgtg agagggtgtc gcctgcaagc
     7561 gccagtgcag cggcgccctg caacaggcca tcgccatcca gcgtggtcgc cctgacgttc
     7621 agcgtgtcag cggtgatttt acccggattg ctgagggagg cagtgtttaa cgttaaacca
     7681 ttatcaccgg tgataagccc gctgttgcgg atgtccggta tatccagcgt caggtctgca
     7741 gcactgtaca gcgtgccgtt ctgctgatta tcaagcctct gtgtgttaac ggtgagtgag
     7801 gcctccccct gcaacagacc gctgttggtc agggtctgtg actgtgtatt cagggcggaa
     7861 gcaacaagta cgccgctgct ggtcagttcc ggcgcactga ggctgagcga cggggcgctg
     7921 cttttcccgc tgtgggtgag cttttcactg gcgttcaggg tgaggatatc tctggcagcc
     7981 tgtgttccgt caagctgtgc gttctgcaca tccacgctga cactgttgcc ctgcgtctgc
     8041 gcagtggtcg tggtcttcag actgttgcct tttacagaca ccgtgccacc cgcactggtt
     8101 tcacccgcca gtgtggaaga cgcgctgttc agcatgaggt taccgtcact gtttgtgacc
     8161 gatttttctg tggtggtcag tgtgtctgca gaagccacaa gttcctttgc cccggagagg
     8221 gtaccgctgt tgtttatctg cgtggcactg agcgtcagca cgtcatcgct gatgagtctg
     8281 ccgcggtttt cgagtgtacc gctgacggta acgcgggcac tgtcttttgc accggcatca
     8341 ccggacagtg tggcattgcg ggcgctgata tcgagtgtgc tgccactttt aatactgccg
     8401 gtgctggtca ggtcagtggc tgtgactttc aggccgttcc cctggacggc accggtatta
     8461 ctgaatgtcc cggttttcac cgtggcatcg cgcccggcaa ccagtgtccc gccgttgttc
     8521 aggctgccgg agctgacggt gagtgttttc ccggcaacgc tacggccttt cagtgtggta
     8581 tcatcggtgg tggtaagggt gacattttca gctgcattca cggccccgga agtctggttc
     8641 aggtgcagtg cactgagcat ggcgttctgt ccggcagtca gttcaccacc gttctgtaca
     8701 atctgcccgg atgaagacag caccagattt ttatccgtca ccagacggga ctgattcagt
     8761 gtcagtcccg ttgtcccggt aacgctgaga tttcctccgg ctttgttatc ccctgaaagt
     8821 gagacatccg ttccggttac ggtggtactg ccgccggcaa ggctgttctt caggaccagt
     8881 tttccggaac tgctcagtat gatatcgccc tcgcgggcat aaaggttacc aagattaacc
     8941 ccgacaccac tttcagtgga ggtcagatga atacgcctgg cgtacattcc accgagagcg
     9001 ccggtatcaa cggcaacttt cggcacatta ccttcgccct tcagggcact gacgcgacca
     9061 tctgcagtta tccggttagc gcctgcagtg acagttaaat ccttcgcatg aagcgcggca
     9121 ttcacttccg ttgcacgggc aataatggat acggcatcgc tccaggtgcc gtccaggccc
     9181 gcgccattga tggtgatact gccttcagtc acctccagcg cctgcaggct gccgtcggca
     9241 ttcatcacag gtttgcctgt ggtgagcgtc gcgtgcggcg tgttgataaa accacagccg
     9301 tcacaggtga taccatacgg gttggcaacc atcacattcg ccgctttgcc ggccacttcc
     9361 gtatagccct gcagcaatga acggttaccg ccagtcactt cgttgatgat acccttcgct
     9421 tccccgcccg ctttcaggtt cgggttattc tgtatcagtc caccaagctg cgtcggatta
     9481 agcttaccgg tggcattatt gagaatcagc ccttccttcc cgacgttgta atccgtaaac
     9541 cggttatgcg aaatcccggc cccgttcggc gtggcaatgt tcacgaccgg cacaccattt
     9601 gccgctttat ccattccggc cccgttttgt ggggtgatga cggcccccac agccggtaac
     9661 aacggctgcc cggcgataat cgtactgata aggtaactca gcaggcggta agtgaagcga
     9721 acgggaggct gatgcatggt aataatccct taaaacgcga cggcaacgcg ccagtaaacc
     9781 gtgagatggt caggggcaag ccagtccggg taaaccagag gcagtccggc agtgaacgaa
     9841 ccggaaacat ggccactggt ggtgctgagc ccggcagcag caccccacag cgtgccggac
     9901 gagtacaggt catctctgtc agagtgcagc cagccgccgt ccagtgcagt cactgcacgg
     9961 actgtcccca catatggcag ggagaacaga gaccaggaca gctcatttcg cagataaccg
    10021 ccgttattac cggagatata ctgctcctta aagccacgca ctgaactctc acccccgagg
    10081 ctcagttgtt ccacaccatg aagacggtcc ggtgaccact gggcataagc gctggtcagc
    10141 caccacaccc tgtccgtgac ggggcgctga aaactggcgc ttaatgacca tttccggaac
    10201 tgatttacgg gcaggtctcc ccttttcccg tggtcgcttt ctgcgccgaa ccagggcatc
    10261 ccccgtgtga ataccggatt cagtgttccg acacccccca gaaacttgtg tgtgtgattc
    10321 agcccgacag aaaatgaagt gagtttacgg ctgctgccct gaagcagaac atcatccaga
    10381 taattgtgaa taatgcggtg ctgcagacct ccggtcagtg ctgtcttcat gtccccgtta
    10441 cggaacagga catgcgacag tcccagccgg tgagtctgca ggtctcccgt ggaacgccac
    10501 cgccagcccc ggttatcaat ggtgctgagg tagtcactcc atgaatacgt gtaatccacc
    10561 agggtatagc catacggcag actgacaccg gcggcaaaat tcctcgcatc atgtgacacc
    10621 gaaaagtcac tgctccgtcc cccgctgaca aaccagttgt cagccagccc cagcggatta
    10681 ttaaaggaaa ggacaccatt taactgcccc gtaccggtat tcttctgtcc gctgttgtcg
    10741 atgcccacgc tccctgtgac aggccattcc ggcgatgccg tcagtgtcac caccgaccat
    10801 ccctcacggt caccgggcga tatttcaatc tgtaccggct ccgtacgcag acggttaatc
    10861 tgctccatcc cctgctcaat atcccgcagg ttcagaacct tcccctccat tcccgggaaa
    10921 accatcttca gggtgcggcc aggaaggtca gcgccttccg cccggatttg ctgcagcctg
    10981 ccttccatga ccgttatgtg cagtacgccc cctgaaaggt cctgctctgt cagaaaggcc
    11041 cggctggtga tatatccccg gcgtatatag ccgtccgtca cggcatccgt gaccgcggtc
    11101 agccccgtga tattcagaca ctgattcacc cacggtgcca ccagtctgtc ggtttctgca
    11161 gacgtcagtc gggtggcccc actgacaaca atgcgtgaca ccgtaaagca gggaccttca
    11221 gtaccggcag acatttccgg agacggtatg acagtcagcg gcgcactgcg ctccagcgca
    11281 tcacgctgac gctggttttc atccagcaac tgctgctgtt gctgctgaat tgcactgcgg
    11341 tcacccggag acagcatggc tgccggagac gaaaatgcgg ggataaaaaa accagaaaaa
    11401 acggagaggg agagataaca tatccccgaa gaggcgcggg cgcgccggaa tatatcataa
    11461 caggggacag acacagcccg gaataaaaga gacgttttcg tcagaatgtt gttctgccgg
    11521 tgctgcatgt tctccccgta ttctggcctg tgtaatgtca gtgtaaattc atgtcaaaaa
    11581 tgtgtcagcg ggaaatttac acctgaaaaa gtctgtcatg aaacagcttt atgacaagat
    11641 atcaaaaaaa atgtcaaaaa cgtgatcttt cttcagcttt tcagaatatt gagagcctag
    11701 aggtcaatat aaattctggg acacatatgc atttaccatt aaaaccggca caacaggctg
    11761 ttcagtctca atccatgctt tctgttttat cgatctcaga atatcggcag acaaataaaa
    11821 aaggaatgtc gagagagcta actctagatc actctccacc tcacaagcca tttacaaaaa
    11881 tgatacaggc atatctttgg tccctgccac aaccagacat tgacaatcca atataacccc
    11941 aaatatattt cggatacgaa gcttgggatt tatacagaca tttactattt aattataaaa
    12001 tttgtattac atacataaga ggcactaatg aaacgttata taaaatggct tgccatcaca
    12061 atttttatca gtatgttgag tgcctgtgcc cgtacggctc cagtgcaaca gataagcacc
    12121 actgtcagtg tgggtcatac tcaggagcag gttaaaaatg ccattttgaa agcaggtgca
    12181 cagcgcaagt ggattatgac gcaagtgtcc cctggagtta ttaaagctcg ctatcaaaca
    12241 cgaaatcacg ttgctgaggt tcgcattaca tatacagcta cttactataa catcaaatat
    12301 gacaatagcc tgaatctgca ggcttctgat ggaaaaattc ataaaaacta taaccgttgg
    12361 gtgcgtaacc tggataaaga tatacaggtt aacttgtcta caggagcaac gttatagtga
    12421 tgctgatatc gggctggaat agcccgatat cattaaactt ctctataaga atggttatat
    12481 atgtacgaac aggacacact cagcacactg gatgccatca cggaggcaca acggattgct
    12541 tttgctccta tgcttttcca ggcagctttg tgcctgcgca atgcaggaat attagattat
    12601 ttggatcaac agggtaaaca gggagctccc cttaacgcga ttactgaaca gacagtcctg
    12661 aatgaatatg ccgtgagtgt gttactggat atggggttaa gcgggcgaat tattacctgt
    12721 aaagagggaa attattacct ggcgaaaatc ggtcattacc tcctgcatga cactatgact
    12781 cgcgtgaata tggatttcac ccaggatgtc tgttaccagg ggcttttctt ccttgccgat
    12841 tcactcaatg aaggaaaacc ttccgggtta aaagtatttg gcgactggcc gacgatatat
    12901 ccggcacttt cgcaattacc agatgctgct cgtgctagct ggtttgcatt tgatcattac
    12961 tattcagatg gcgcatttaa cgccgcactg ccttatgtat tcgccaacaa ccccacaacc
    13021 ttgtacgatg tgggcggcaa tacgggtaag tgggctttgc gctgctgcaa atacaatgaa
    13081 aacatcgccg ttacgttatt agatctgcca caacaaatcg tactggcgaa agaaaatatc
    13141 gcgaatgcag gtttttctga tcgcatagat ttccaagcgg tggatatgct aagcgatgcc
    13201 ccgttaccag gcgaggctga tatctggtgg atgagccaat ttttagactg tttttcaccc
    13261 gaacaaataa ttagcatact cagcaaaatc gccagcgtaa tgaagcctgg cgcaaaagtt
    13321 tgcatcatgg agttgttctg ggatgcgcag cggtttgaag cagcatcgtt tagcctgaac
    13381 gcgtcctcac tttattttac ctgcatggca aacggtaaca gccgtttcta tagtgcagag
    13441 aaattttacg actatctgaa taaagcagga ttccaggtag cagagcgcca cgataattta
    13501 ggcgttggac atacactttt gatatgccaa aagaaataat cgtcacttaa taattttggt
    13561 ttaccgcggg cacgcgttga tgaaatttgc attaaatatt accaactggc aggcgctggc
    13621 gcctggactt agcgatgtgc agcaatggca agcatggtcg cgccagccgt gggcgattga
    13681 ccctgccgcg ccgctggcaa aattaagtga actgccaatg atgactgccc gtcgtctcag
    13741 ttcaggcagc aaactggctg ttgaatgcgg tctggcaatg ctacgccgct atcagcctga
    13801 cgccgttttg tataccagtc gtcatggcga actggagcgt aattaccgca ttgttcatgc
    13861 actggcaacc gaacaggcgc tttctccgac ggacttcgcg ctttccgtac acaattcttc
    13921 cgtgggtaac ctgaccattg cggccaaaca gccgatcgtt tcgtcatcgc tttcagctgg
    13981 tcgcgacagt ttccagcaag gtttgtgtga agtgcttagc ctgctacaag cgggatacca
    14041 gcgcgtgttg atggtcgatt ttgacggttt tctgcccgag ttttatcacc cacaactgcc
    14101 tgcagaaatg ccgacctggc cgtacgccgt cgcgctgatg attgaatctg gcgatgactg
    14161 gcaatgtgaa acgcagtcag ccattgcggt aaatgaaaca acactgccgc aaagcatgtt
    14221 gtttttacag cactatttac aaaatgcagg cgcattttca ctgtctggcg agcgcgtgca
    14281 atggcgctgg agccgcagat gaataagctg atgtcgcgcc tgggatgggc atggcgactg
    14341 gtcatgaccg ggttatgttt tgccctgttt ggtctcggcg gcctgttgct ttcagtcgta
    14401 tggtttaaca tattgttagt tttggtatgg gatacttccc gccgccgtcg tttggcgcgt
    14461 cgcagcattg ccgccagttt tcgcctgttt ttaactgtcg cgaaagggct tggcgtgctg
    14521 gattatcgta ttgatggcgc tgaaatttta cgtcaggaac gcggttgcct cgtcgtagcc
    14581 aatcatccca cgctgatcga ttacgtgctg ctggcgtcag tcatgccaga aactgactgc
    14641 ctggtgaaaa gcgcgctgtt aaaaaatcct tttcttggcg gcgtcgtgcg ggcggcagat
    14701 tatttaatca acagcgaagc tgaaaccctg ttaccccgct gccaacagcg actggcacag
    14761 ggtgacacta tcctgatttt ccccgaaggc acacgaacga aacccggtga aaaaatgaca
    14821 ttacagcgcg gtgcagcgaa tattgccgtg cgctgcgcca gcgatttacg catcgtgaca
    14881 atcggctgta gtgagcatct gctggataaa caaagtaaat ggtatgacgt tccgcctgcc
    14941 aggccgtttt ttacggttga agtccatggg cgggtagaaa tcaataaatt ttacgatgca
    15001 acttcacatg agccggcgct ggctgcaagg cagttaaacc gacatctttt gcttcaatta
    15061 caacaaggtt gtttacctct ctcaggaata aataatgcaa gcgctttatc tggaaattaa
    15121 aaatctcatc atcagcaccc tcaatctgga cgagttgact cctgatgata ttgataccaa
    15181 tgcgccactg tttggcgacg gtttagggct ggactctatc gatgcactgg aactggggct
    15241 ggcagtaaaa aatgagtacg gcgtcgtgct ctctgcggaa agcgaagaaa tgcgtcagca
    15301 ctttttctcc gtcgccactc tggcctcttt cattgctgcg caacgtgcct gaggaaagcc
    15361 atcatgacag accaacaaac catttatcag gaagtctcag ccctgctggt taagctgttt
    15421 gaaatcgatc cgcaggacat caaacctgaa gcgcgcctgt acgaagatct ggaactggac
    15481 agcatcgacg ccgttgacat gattgtgcac ctgcaaaaga aaaccggtaa gaaaatcaag
    15541 ccggaagagt ttaaagcagt gcgtacagtc caggatgttg tagaggctgt aaaacgcctg
    15601 ctacaagaag gctaatttga tgtcgggcat tcgctcgcta ccgatgatta agttgctgac
    15661 cggattattg ctgctcgcct ggcctttttt aatctggctc ggtttggcgc ataacagcct
    15721 gcactggctg tcgccgctga tggcgttgct gcttttattg cgcctgcgcc agccccgtcg
    15781 tcaggcaggg ccgctacaag ccgtcacaaa aatcgtcgct gttgtgggca ttgtattgtg
    15841 tgtctccagt tttctgttga aaacgcacca gttactgctg ttttacccgg tagtggttaa
    15901 cgctgtcatg ctggcggttt ttggcggttc gctgcggtcg acaatgccga tagttgagcg
    15961 gctggcacgt ttgcaggaac cagacctccc ggaaaaggcc gtgcgatata cccgccgcgt
    16021 aacgcaggtc tggtgcctgt tttttatttt caacggcagt attgcgttat ttaccacctt
    16081 gtatggcaac atgcctttgt ggacagcctg gaacggtatc attgcttacc ttctgatagg
    16141 tttcctgact gcaggggaat ggctgatacg ttgtcagatg atcaaacgga aaacaccatg
    16201 aatcagactc tctcactttc acagtggttg acagcctctc gccctgtaac aactcctgtt
    16261 gcctggcttg gggaatatac ctggacactg gggcatctgc ggcatgatgt agcgcttttg
    16321 atcgaccatt tacgcgacca gccaggcaat cgctgggcac tttgttttga aaacagctat
    16381 ctttttattg tagcgttgtt ggccacgcta cactcaggta aaatcccagt aatcccgggc
    16441 cataatcgcg ccgctttgtt aaatgtacaa cgctccttgt ttgattgcat cctgagtgat
    16501 aaaaggctgg gctggaacgg tccacagttt gttgtaagca catctcacca gatgacgacc
    16561 agcacgatag cctttgatga tattgcttct gacgcgttta ttgaactttt cacatcgggt
    16621 tcaaccggac aaccaaaaca aattgcaaaa cctgttatca gccttgatca agaagcaaac
    16681 atgctggcaa ctcactttgc agaccgacta ctgggctgtc gttttgtagc atccgtgatg
    16741 cctcagcacc tgtatgggct gactttccgt atttttttac ctatggcact tggcctgcca
    16801 ttgcatgccg caatgctctg gtattctgag caacttgccg cattaagcca tacctaccgc
    16861 tatgcctttg tcagcagccc ggcgttcctt aaacgactcg accttcatct gacgcctccc
    16921 cccgtggaaa tgattctctc tgccggcggt atgctaccat ggcaggatgt agagaaaact
    16981 tccacctggc tgaatatctg gcccgatgaa atctatggta gtaccgaaac aggtattctc
    17041 gcatggcgtt accgtcaaca ggataatatc ccctggttta ctttttctga tgttcatctt
    17101 tcacaggaaa gtgagggggt tcgtgtcttt tcgccactca tccctgctga aggattggtg
    17161 cttgacgata tgttgcaatt caatgaaaac ggtcagttcc acctgatcgg acgacgtggt
    17221 cgagtggtaa aaatagagga gaagcggata tcactggttg aaatcgaaca acggcttctg
    17281 gcacttgacg gtattatgga tgtcgctgca ataccattaa tacgcaatgg ccgccaggct
    17341 atcggtgtcg tggttgtacc aaataaagag gcccgtcaaa tatggcaatg ctccggaggg
    17401 aaaactctgg agttgtcctg gcgcaaagca ctattaccat ggcttgaacc tgttgcagta
    17461 cctcgttact ggcgcattat tgatgaaata ccagtaaaca atatgaataa gcgtgtctat
    17521 gcacaattac aggagttatt tcatgatacg ccatgaaatt gagcgccatc aggttcaact
    17581 gcaggagatg gaaatcgttc tataccttga cccgatgctc tactggttta atggtcattt
    17641 tgccgtacag cctctgttac caggcgtggc ccagttggac tgggtaatgc attatgctac
    17701 cacgctgctt gctccgggct ggcgttttcg tagtatccag aatgttaaat ttctggcgcc
    17761 acttatacct gagacgactg ttaccctgca attaacatgg caggaaacaa gtcaggtact
    17821 cactttctgc tatcagcgtc acgacggtga cgcccgccac accgccagta gcgggaagat
    17881 ccgcctatgt cggtaaactt ttctccctgc gtgttgatcc cctgctacaa ccacggtgcg
    17941 atgatgccgg gcgtgctggc gcgtcttaag ccatttaatc ttccctgtat tgtggtggat
    18001 gacggcagcg atgccgccac gcaacagcaa ctggataatc tggttgccga acagcctggc
    18061 gtgaccttaa ttcgcctggc agaaaacaca ggcaaaggcg cggcggtaat gcgcggctta
    18121 caggcggcgg cagacgcggg cttcagccat gcggtgcagg tggatgctga cggtcaacat
    18181 gcgattgaag atatccccaa actgctggca ctcgctgaac aacaccctgc ggcgctgatc
    18241 tccggccagc caatttacga tgactccatc ccccgctcac ggctttacgg gcgctgggtc
    18301 acccacgtct gggtatggat cgaaacgctc tccctgcaac tgaaagacag tatgtgcggc
    18361 tttcgcgttt atccggttgc gccaacgctg caactggcaa aatacgccac catcggcaag
    18421 cggatggatt tcgacactga agtgatggtg cgcctctact ggcagggaaa cacctgttat
    18481 ttcgtgccaa cccgcgtcac ttatccgcag gacgggcttt cgcattttga tgccctgaaa
    18541 gataacgtcc gcatctcgct catgcacacg cgtctgtttt tcggcatgtt gccgcgtatt
    18601 ccttcactac tgatgcgccg ctcttccagc cactgggcgc ggcagagtga agtgaaagga
    18661 ttatggggaa tgcgcctgat gctgctggtc tggcgtctgc tgggaagaac ggcgtttagc
    18721 gcgctgcttt acccggtggt gggcgtctac tggctcactg ctgctcgtgc gcgcaaagcg
    18781 tcgcaagact ggctcgcccg tgtacgacag catcaaccac aggcggcaaa actcaacagc
    18841 tatcagcact ttctacgttt cggtaatgcc atgctcgaca aaatcgccag ctggcgcggc
    18901 gagctacaac tggggcacga tgtgctgttt gcgccaggcg cagaagcggc gcttgacgtc
    18961 cgcgatccgc gcggcaaatt gctgctggcc tcgcatcttg gcgatgtgga agtgtgccgg
    19021 gcgctggcaa aaattcaggg ctacaaaacc attaacgcgc tggtgtttag cgaaaacgcc
    19081 caacgcttta aacagataat gcaggagatg gctccccagg caggcattaa cctgatgccg
    19141 gtaacagata tcggcccgga aaccgccatc ctgctgaaag agaagctgga taacggcgaa
    19201 tgggtggcga ttgtcggtga ccgcatcgcc gtcaccccgc aacgcggcgg cgactggcgc
    19261 gtctgctgga gtccgtttat gggccagcct gcgcctttcc cacaggggcc gtttattctc
    19321 gcgtctattt tgcgctgccc ggtgaatctg attttcgccc tgcgccagcg cggcaagctg
    19381 catattcact gcgaaacctt tgccgacccg ctgctgttgc cgcgtggcga acgccaacag
    19441 gggctgcaaa acgctatcga ccactacgcc gcgcgtctgg aacattacgc gctccagtcg
    19501 cccctcgact ggtttaattt tttcgatttc tggcaactgc cggaaattca ggacaaggag
    19561 taaagggtgc ttaacgatcc ccgatttacc gctgaagtcg agctgaccat tccctttcac
    19621 gacgtcgata tgatgggcgt ggcctggcac ggtaactatt ttcgctactt tgaagtggcc
    19681 cgcgaggcgc tgctcaacca gttcaattac ggctatcggc agatgaaaga gtcaggttat
    19741 ttgtggccag tggtcgatgc gcgggtgaaa tatcgccacg ccctcacctt tgagcaacgc
    19801 atccgcgtgc gtgcgcatat cgaagagttc gaaaaccgtc tgcggattgg ctatcagatt
    19861 ttcgatgccg aaacgggcaa acgcgccacc acaggataca ccattcaggt cgcagtagag
    19921 gagcaaagcc gtgaactgtg ctttgtcagc cccgatattc tgtttgaacg catgggagtc
    19981 aaaccatgaa atttttaccg ctgctggcgc tgctgattag cccgtttgtg agcgccctga
    20041 ccctggacga tcttcagcaa cgctttaccg aacaaccggt gatccgcgcc catttcgatc
    20101 aaacccggac gattaaagat ctgccgcagc cgctgcgatc tcagggtcag atgttgatcg
    20161 cccgcgacca ggggctgttg tgggatcaaa cttcaccatt ccccatgcag ctattgctcg
    20221 acgataaacg catggtgcag gtgatcaacg gccagccgcc gcaaatcatc acggcagaaa
    20281 ataacccgca gatgttccag tttaaccacc tgctgcgcgc gctgttccag gccgatcgca
    20341 aagtgctgga acaaaacttc cgcgtcgaat ttgccgataa aggcgaaggc cgctggacgc
    20401 tgcgcctgac gccgaccacc acgccgctgg ataaaatttt caacaccatc gatctcgccg
    20461 ggcaaaccta tctggagagc attcagctta atgataaaca gggcgatcgc accgatatcg
    20521 ctcttaccca acatcaactg acgccagcgc aactgagcga tgacgaacgc caacgttttg
    20581 ccgcccagta aacgccccgc gctgttatgg gggctagtct gcctggtcat ggcggtggta
    20641 ttgctgatcc tgctgccgca atcacggctg aacagtagcg tgctggctat gttacccaaa
    20701 caggcgatgg gcgatattcc cccagcgctg aatgacggct ttatgcagcg tctggaccgc
    20761 caactggtgt ggctggtcag ccccggtaaa gaggctaatc ccagggtggc tcaggaatgg
    20821 ctgacgctgc tgcaaaagtc cgctgcgctt ggcgacatta aagggccaat ggatgccgcc
    20881 agccagcagg cgtggggggc gtttttctgg cagcaccgca acggcctgat tgaccccgac
    20941 acccgcgccc gcctgcaaaa cggcggcgaa gcgcaggcac aatggatcct ctcccagctt
    21001 tattctgcgt tctccggggt aagcggcaag gagttgcaaa acgatccgct gatgttaatg
    21061 cgcggctcgc agctggcgat ggcgaaaaac ggccagcgtt tacgactgat ggacggctgg
    21121 ctggtgacgc aggatcccca gggcaactac tggtatctgc tgcacggcga actggcggga
    21181 tcgtcgtttg atatgcagca aacccaccag ctcatcacga ccctgaatac gctggaaaaa
    21241 gatctgaaaa cgcgttaccc gcaggcgcag ttgctctcgc gcggcacggt gttttacagc
    21301 gattacgcca gccaacaggc gaagcaggat atctccaccc tgggcgtggc aacgctgctg
    21361 ggggtgatat tgatgattgt ggcggtgttc cgctctttac gcccgttgct gctttgcgtg
    21421 atttccatcg gcatcggcgc gctggcggga acggccgcca cattgttgat tttcggcgaa
    21481 ttacacctga tgacgctggt gatgagcatg agcgttatcg gcatttccgc tgactacacg
    21541 ctctattacc tcaccgaacg gatggtgcac ggcaacgacg tttcgccgtg gcaaagcctg
    21601 gcgaaagtgc gcaatgcctt gctactggcg ctgctcacca ccgtggcggc atatctgatt
    21661 atgatgctcg ccccttttcc cggtattcgg caaatggcga tttttgccgc cgtcgggttg
    21721 agcgcctcct gtctgaccgt cctgttctgg catccgtggc tgtgccgtgg cctgccggta
    21781 cgcccggttc cggcgatggc gctgatgctg cgctggctgg cggcgtggcg gcgtaataaa
    21841 aaactgtcgc tgggtctgcc cgtcgcgctg gcgctgtttt cgctggcggg gatgtcaatg
    21901 ctacgcgtcg atgacgatat ctcgcagtta caggcgctgc cgcagcatat tctggcgcag
    21961 gagaaagcca ttaccgccct gaccggacaa agcgtcgatc aaaaatggtt tgtagtttac
    22021 ggcgactccc cacagcaaac cttgggacgg ctggagacat ttaccacctc gctggagaaa
    22081 gcgaaaaaag acggccttat cagcaactac cgcaccattc cgctgaactc cctggcgcgg
    22141 caggaggaag atttagacct gctgaaaacg gctgccccga cggtaacaaa agcactgcaa
    22201 aatgccgggc tgacggcggt gaacccggat cttaacgcca tgccagtgaa ggttgatgaa
    22261 tggctggcaa gccccgccag tgaaggctgg cgtctgctgt ggctgacgct ggaaaacggc
    22321 gaaagcggcg tactggtgcc ggttgaaggg gttaaaagta gcgcgttgca gcaggaaatc
    22381 gccagctatt accctggcgg cattgcctgg gttgatcgca aaagcacctt tgatgaattg
    22441 ttcgcacttt accgttatgt gttaaccggc ttgttgctgg tggcgctggc agtaattgcc
    22501 tgcggcgcag tggcccgcct cggctggcgc aaagggctta tcagcctggt gccttcggtg
    22561 ctttcgctgg gctgcggtct ggcagtgctg gcgatgagcg ggcaggcggt gaatctcttt
    22621 tcgctactgg cgctggtgct ggtgcttggc atcggcatca attacacgct gtttttcagt
    22681 aatccgcgcg gtacaccgtt aacttcccta ctggcgatca cgctggcaat gctcaccacc
    22741 ttgctgacgc tgggaatgct ggtattcagc gccacccagg ccatcagcag ttttggtatt
    22801 gtgctggtga gcggtatttt caccgccttc ctgctttcgc cgctggctat gcccgataaa
    22861 aagagaacaa aaaaatgatc aaatccacct tctggcgagc gctcgccctg accgctacgc
    22921 ttatcctcac cggctgtagc cactcgcaac cggaacagga aggccgcccg caggcgtggc
    22981 tgcaacctgg cacacgcatc acgctgcctg cgccggggat ttcacccgcc gtcaattccc
    23041 agcaactctt gagcggcagc ttcaacggca aaacccagtc gctgctagtg atgcttaacg
    23101 ccgacgacca gaaaatcacc cttgccgggc tgtcgtcggt cggcattcgc ctgtttctgg
    23161 tgacctacga tgcaaaaggt ctacgcgccg agcaatccat cgtcgtcccg cagctgccgc
    23221 ccgcaagtca ggtgctggct gacgtaatgc tcagccactg gccgattagc gcctggcaac
    23281 cgcaacttcc cactggctgg acgcttcgcg acaacggcaa caaacgcgag ctgcgtaacg
    23341 ccagcggcaa gctggtcacg gaaatcatct atctgaatcg ccagggcaaa cgcgtgccaa
    23401 tcagcattga gcagcatgtc tttaaatacc acatcaccat tcaatactta ggtgactgat
    23461 atgatttata tttccgccgt cggcatgatc aacgccctgg gaaataatct tgacgaaatc
    23521 gcagccaacc tggctcgcgg tgtcgccccc ggcatgcgtc cgcgcgcagg ctggttgcag
    23581 gggcatccgc aggcggtcct ggctggcgtg gatggcgagc ttccgcttat ccccgaaaaa
    23641 ttcgctgcgc accgctcgcg caacaatcag atcctgctgg cggcgctggc gcagatccag
    23701 ccgcaggttg atgacgctat cgcgaaatat ggccgcgagc gcatcgcgat tgtgcttggc
    23761 accagcacct ccggcctgca tgagggcgac acgcacgtta acctgcgcac caacggacag
    23821 ccgagcacca catggcacta cgcgcaacag gaactcggcg atccgtcgcg ttttctcagc
    23881 cactggctgg cgctcgacgg cccggcctat accctttcaa ccgcctgctc ttccagcgcc
    23941 agagcgatga tcagcgggcg caggcttatc gaagcggggc tggtcgatgc cgccattgtg
    24001 gggggcgcag atactctgag ccggatgccg attaacggtt ttcacagcct cgaatcgctc
    24061 tcgccaacct tatgtcagcc gtttggtcgt gaccgcgcag gtatcaccat tggcgaaggg
    24121 gcgggactga tgttattaac ccgtgaaccg cagcccatcg cgttactggg cgttggtgaa
    24181 tcgagcgacg cttaccatat ttcggccccg catccgcaag gcgaaggggc gatccgcgcc
    24241 attaaccagg cgctgaccga tgcgcagctt acgccagatg acatcggcta tatcaacctg
    24301 cacggtaccg ccacacaact caacgaccag attgaatcaa tggtggttaa cgccctgttt
    24361 ggcaaacgca taccgtgcag ctccaccaaa catttgaccg gacacacgct cggcgcggct
    24421 ggcattaccg aggcggcaat tagtatgttg attttacagc gtgatttacc gctgcccgct
    24481 caggacttca gcctgtcgcc gcgcgatccc acgctgccac cgtgtggcat tatcgaaaaa
    24541 ccgcagccgc tggcacgtcc ggtgatcctg tccaattcct tcgcctttgg cggcaataac
    24601 gccagcattc tgctcgggag ggtttcgtga gccactattt atcccccggc gcttatctgc
    24661 cccacgacgc gcccatgtta ctgctggaag acgtggtgag cgtcagcgat gacagcgcgg
    24721 tgtgccgcgt gacggtttcg cccagtggcg tactggcacc gtttctcgac ccggacggca
    24781 atctccctgg ctggtttgcc cttgaactga tggcgcaaac cgtcggcgtc tggtctggct
    24841 ggcatcgcca ccagcagggc aaaaacagta ttgagttagg gatgatactg ggcgcgcgtg
    24901 aattgctttg caccactggc attctgcccg caggccagac gctgaccatt accgtcaaac
    24961 tgctgatgca ggacgatcgc ttcggcagct ttgagtgcag catcaacgac ggcgaggcga
    25021 cgggccgcat taacaccttc cagccgaccg cggaagaact taccaccctg tttcaacaag
    25081 gagcgtcctg atgagtcgtt cagttctggt tactggtgcc agcaaaggca ttggtcgcgc
    25141 catcgcctgc aagcttgcgg cagacggctt taacattggc gttcactatc accgtgacgc
    25201 cacaggcgct caggaaacgc tgaatgccat tgtcgccaac gggggtaacg ggcgtttgct
    25261 cagtttcgac gtcgcaaacc gcgaacagtg tcgggaagtg ctggagcatg agatcgcgca
    25321 acacggtgcc tggtacggcg tggtcagtaa cgccgggatc gcccgcgatg ccgcttttcc
    25381 ggctttaagc gatgacgact gggatgcggt gatccacacc aacctcgaca gtttctacaa
    25441 cgtcattcag ccgtgcatta tgccaatgat cggcgcgcgc cagggtgggc gcatcatcac
    25501 gctgtcctcg gtttccggcg taatgggcaa tcgcggccag gtgaattaca gcgcagccaa
    25561 agccggaatt atcggcgcaa caaaagcact ggctatcgaa ctcgcaaagc gcaaaatcac
    25621 cgtcaattgc atcgcgccag ggcttattga taccgggatg atcgaaatgg aagaaagcgc
    25681 tctgaaagag gcaatgtcga tgatccccat gaaacgtatg ggccaggcgg aagaagttgc
    25741 cgggcttgcc agttatttaa tgtctgatat tgcgggttac gtcacccgcc aggttatttc
    25801 catcaacgga gggatgttat gacacgtcgc gtagtgatta caggaatggg cggcgtgact
    25861 gcctttggtg aaaactggca ggacgtttct gcacgcttac tggcgtatga aaatgcggtg
    25921 cgcaaaatgc cggagtggca ggtctacgat gggctgcaca ccctgcttgg cgcgcctgtg
    25981 gatgatttca cgctaccgga gcactacacc cgcaagcgta tccgtgcgat gggccgcgtg
    26041 tcgcaaatgt cgacccgtgc cagcgagctg gcgttagagc aggcagggtt gattggcgat
    26101 ccgatattaa ccagcggcga aacgggcatt gcgtacggtt catcgaccgg cagtaccggt
    26161 ccggtgagcg aatttgccac catgctgact gaaaagcaca cgaacaacat caccggcacc
    26221 acctatgtgc agatgatgcc gcacaccacc gctgttaata ctggcctctt tttcggcctg
    26281 cgcgggcggg tgatcccaac ctccagcgcc tgtacctccg gcagccaggc gatcggttat
    26341 gcgtgggaag ccattcgcca cggttatcaa acggtgatgg ttgccggtgg tgccgaagag
    26401 ttatgccctt cagaagcagc ggtgtttgat accctttttg ccaccagcca gcacaatgat
    26461 gcgccgaaaa ccacaccttc accgttcgat gaaaaccgcg acggactggt gattggtgaa
    26521 ggcgcaggta cgctcattct cgaagaactg gagcatgcca aagcgcgcag cgcgaccatt
    26581 tacggtgaaa tcgtcggctt tgcgactaac tgcgacgcgg cacatattac ccagccgcag
    26641 cgcgaaacca tgcagtattg tatggagcaa tcgctaaaaa tcgcagggtt aagcgcacaa
    26701 gatatcggct atatttcagc gcatggtacg gcgaccgatc gcggtgatat ggcagaaagt
    26761 ctggcgacag caaccattta cggtgataat gtgccgctct cctcgctgaa aagttatttt
    26821 ggtcatacgc ttggtgcctg tggcgcgctc gaagcctgga tgagtctgca aatgatgcgc
    26881 gaaggttggt ttgcgcctac gctaaacctg aataaaccag acccgaattg tggcgcgctg
    26941 gattacatca tgcatgaagc ccgcaaggtt gattgtgagt ttttgcagag taacaacttt
    27001 gcctttggcg gcattaatac atccatcatt attaagcgct ggccctgagt tatacaacca
    27061 catggacatc ccatgtggtg attcttcaaa aaatctgaat caatgaatat gaacctcaaa
    27121 ttctcttacg catactgaag agcattccgt ataacgattc agcagacaaa actttatcag
    27181 tccacacgaa tcaacggatg cacagatgac acgcccggtt atcaccgggc aatggttgcc
    27241 ataacgggta acataaggga ctgaacagtt gtctttagcg caacgaactg atctaacgta
    27301 cctgaacgga caacctgaaa acagccttcc atcctgcatt gtccgccagt gcttattacc
    27361 aaccacgtct gttgcagtaa agtaatcagt tgcgaacatt atttatcaat cgtgacgctg
    27421 gctcgcgcat ggcagaaaag gtcctgactg agcaacaaca ggcatcgtac tgttgctgct
    27481 ccatgttgtt tatatgaacg catcccgttt gcaaggcttc cgtgtttttg acgttgacgg
    27541 tagattgctg ctctatatcc ttattgcagc cggtgctgcg ggcctcaatg agattcgctg
    27601 actctcgcct tattcacgca acgagcttta agctcagaaa aacatccaag tttggtgagt
    27661 cacagtctta cctgtcgttg tcatcaatat aggaatggat aatctggaga gcgatactgc
    27721 aggctgatat tgcggttttg cgacaagttt ttagcatccg cttctacgcc aaatacctga
    27781 agtaggaatg gatgcgttag cagcatacat ccggaactgc gcggggaata gccggaaacg
    27841 gctatcccta ccacaatagc taactttgta atagcgctct tttaggctca tgtactctga
    27901 cgtaattcta tctggtagtt gaattgcacg atacgcgact cttctgattc atcatgatta
    27961 ttattcaata gtccaatcag taacttacct gtctctatcc ccattttttt gtagtcaata
    28021 cggatggatg tcaaagttgg gtgtgtttga tcgcaattgt ctgaaccgtc aagacaagct
    28081 acagcgatat ttccagggat ttttaacaaa cgccgctggc actcaaacat tatacccagc
    28141 gcaatctctt cgtggctgca aatcacagca tccagctctg gttgtcgcag taatatctcg
    28201 tttaatgcgt aacgccccaa ttgcagactt gcggcatccg gcatggtgat aacaagatca
    28261 gtgttctggt aatggctcag cattgcttta tgccagccgt tcagttgctg acgttgcagg
    28321 cggttgtcca tatgcgcacc gataaatccg atattcttat aaccctgctc cagcagatgt
    28381 tcggtcagtt tatgtgatgc ttcaaaaaat gctgtttcga tggtaatttg agccggactg
    28441 aaaggggaac cgataacgtt aatggtaggt atatttgttt tctcgagtat ctggtgagtt
    28501 ttatcagcca actgagaacc aaaaataact aacgccgccg ggttgccttg aagaagcgtc
    28561 atcagaattt cagcttcttt gagctgatta tactcatgac acccaagcag tagctggaat
    28621 tcatttttat taagcacctg ctgtaatgac tgcataaagc gggagctggc tttatctgtc
    28681 agggaaggaa ccagtacggc gactgtat
//
LOCUS       DAZUCC010000040        28452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZUCC010000040 DAZUCC010000000
VERSION     DAZUCC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.040"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            91..1530
                     /gene="norV"
                     /locus_tag="ACHY09_001562"
     CDS             91..1530
                     /gene="norV"
                     /locus_tag="ACHY09_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFS3884549.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLTDIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            1527..2660
                     /gene="norW"
                     /locus_tag="ACHY09_001563"
     CDS             1527..2660
                     /gene="norW"
                     /locus_tag="ACHY09_001563"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFS3884550.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQRQRADDLTRQTAGEFAEQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRNARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILAALMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(2884..5136)
                     /gene="hypF"
                     /locus_tag="ACHY09_001564"
     CDS             complement(2884..5136)
                     /gene="hypF"
                     /locus_tag="ACHY09_001564"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFS3884551.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAGLGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(5289..5816)
                     /gene="hydN"
                     /locus_tag="ACHY09_001565"
     CDS             complement(5289..5816)
                     /gene="hydN"
                     /locus_tag="ACHY09_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFS3884552.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(5965..6975)
                     /gene="ascG"
                     /locus_tag="ACHY09_001566"
     CDS             complement(5965..6975)
                     /gene="ascG"
                     /locus_tag="ACHY09_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFS3884553.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIAL
                     SR"
     gene            7235..8692
                     /gene="ascF"
                     /locus_tag="ACHY09_001567"
     CDS             7235..8692
                     /gene="ascF"
                     /locus_tag="ACHY09_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFS3884554.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            8701..10125
                     /gene="ascB"
                     /locus_tag="ACHY09_001568"
     CDS             8701..10125
                     /gene="ascB"
                     /locus_tag="ACHY09_001568"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFS3884555.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LARTRKKSFWWYKKVIASNGEDLE"
     gene            complement(10283..10753)
                     /gene="hycI"
                     /locus_tag="ACHY09_001569"
     CDS             complement(10283..10753)
                     /gene="hycI"
                     /locus_tag="ACHY09_001569"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFS3884556.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(10746..11156)
                     /gene="hycH"
                     /locus_tag="ACHY09_001570"
     CDS             complement(10746..11156)
                     /gene="hycH"
                     /locus_tag="ACHY09_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFS3884557.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(11153..11920)
                     /gene="hycG"
                     /locus_tag="ACHY09_001571"
     CDS             complement(11153..11920)
                     /gene="hycG"
                     /locus_tag="ACHY09_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFS3884558.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(11920..12462)
                     /gene="hycF"
                     /locus_tag="ACHY09_001572"
     CDS             complement(11920..12462)
                     /gene="hycF"
                     /locus_tag="ACHY09_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFS3884559.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(12472..14181)
                     /gene="hycE"
                     /locus_tag="ACHY09_001573"
     CDS             complement(12472..14181)
                     /gene="hycE"
                     /locus_tag="ACHY09_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFS3884560.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(14199..15122)
                     /gene="hycD"
                     /locus_tag="ACHY09_001574"
     CDS             complement(14199..15122)
                     /gene="hycD"
                     /locus_tag="ACHY09_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFS3884561.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(15125..16951)
                     /gene="hycC"
                     /locus_tag="ACHY09_001575"
     CDS             complement(15125..16951)
                     /gene="hycC"
                     /locus_tag="ACHY09_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFS3884562.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(16948..17559)
                     /gene="hycB"
                     /locus_tag="ACHY09_001576"
     CDS             complement(16948..17559)
                     /gene="hycB"
                     /locus_tag="ACHY09_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFS3884563.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(17684..18145)
                     /gene="hycA"
                     /locus_tag="ACHY09_001577"
     CDS             complement(17684..18145)
                     /gene="hycA"
                     /locus_tag="ACHY09_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFS3884564.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            18357..18707
                     /gene="hypA"
                     /locus_tag="ACHY09_001578"
     CDS             18357..18707
                     /gene="hypA"
                     /locus_tag="ACHY09_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFS3884565.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            18711..19583
                     /gene="hypB"
                     /locus_tag="ACHY09_001579"
     CDS             18711..19583
                     /gene="hypB"
                     /locus_tag="ACHY09_001579"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFS3884566.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            19574..19846
                     /gene="hypC"
                     /locus_tag="ACHY09_001580"
     CDS             19574..19846
                     /gene="hypC"
                     /locus_tag="ACHY09_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFS3884567.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            19846..20967
                     /gene="hypD"
                     /locus_tag="ACHY09_001581"
     CDS             19846..20967
                     /gene="hypD"
                     /locus_tag="ACHY09_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFS3884568.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAETHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            20964..21974
                     /gene="hypE"
                     /locus_tag="ACHY09_001582"
     CDS             20964..21974
                     /gene="hypE"
                     /locus_tag="ACHY09_001582"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFS3884569.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            22048..24126
                     /gene="flhA"
                     /locus_tag="ACHY09_001583"
     CDS             22048..24126
                     /gene="flhA"
                     /locus_tag="ACHY09_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFS3884570.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRHDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLSLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            24171..24599
                     /locus_tag="ACHY09_001584"
     CDS             24171..24599
                     /locus_tag="ACHY09_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFS3884571.1"
                     /translation="MAVSARNQLTGTVSTVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(24638..24982)
                     /gene="ygbA"
                     /locus_tag="ACHY09_001585"
     CDS             complement(24638..24982)
                     /gene="ygbA"
                     /locus_tag="ACHY09_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFS3884572.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            25268..27829
                     /gene="mutS"
                     /locus_tag="ACHY09_001586"
     CDS             25268..27829
                     /gene="mutS"
                     /locus_tag="ACHY09_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS3884573.1"
                     /translation="MRTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQHTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLEGVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIATGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            27935..>28452
                     /gene="pphB"
                     /locus_tag="ACHY09_001587"
     CDS             27935..>28452
                     /gene="pphB"
                     /locus_tag="ACHY09_001587"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFS3884574.1"
                     /translation="MPSTRYQKINAHHYHHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNS"
BASE COUNT         6785 a         7803 c         7624 g         6240 t
ORIGIN      
        1 tagagtaaaa acaattagat aaaaaactgg cacgcaatct gcaatcagca agacatcttt
       61 ttagaacacg ctgaataaat tgaggttgct atgtctattg tggtgaaaaa taacattcat
      121 tgggttggtc aacgtgactg ggaagtgcgt gattttcacg gtacagaata taaaacgctg
      181 cgcggcagca gctacaacag ctacctcatc cgcgaagaaa aaaacgtgct gatcgacacc
      241 gtcgaccata aattcagccg cgaatttgtg cagaacctgc gtaatgaaat cgatctgacg
      301 gatatcgatt acatcgtaat taaccatgct gaagaggacc acgccggggc gctgaccgaa
      361 ctgatggcac aaattcccga tacgccgatc tactgtactg ccaacgctat cgactcgata
      421 aatggtcatc accatcatcc ggagtggaat tttaatgtgg tgaaaacagg cgacacgctg
      481 gatatcggca acggcaaaca gctcattttt gtcgaaacgc caatgttgca ctggccggac
      541 agcatgatga cttacctgac aggcgacgcg gtactgttca gtaacgatgc tttcggtcag
      601 cactactgcg acgagcatct gttcaacgat gaagtggatc agacggagct tttcgagcag
      661 tgccagcgtt attacgccaa tatcctgacg ccgttcagcc gcctggtaac gccgaaaatt
      721 accgagatcc tgggctttaa cttaccagtc gatatgatag cgacctctca cggcgtggta
      781 tggcgcgata acccgacgca aattgtcgag ctgtacctga aatgggcggc ggattatcag
      841 gaagacagaa tcaccatttt ctacgacacc atgtcgaata atacccgcat gatggccgat
      901 gctatagccc agggtattgc ggaaaccgac ccacgcgtag cggtgaaaat tttcaacgtc
      961 gcccgaagcg ataaaaacga aatcctgacc aatgtcttcc gctcaaaagg cgtgctggtc
     1021 ggcacttcga cgatgaataa cgtgatgatg ccaaaaatcg ccgggctggt ggaggagatg
     1081 actggtttac gctttcgtaa caaacgcgcc agtgctttcg gctctcacgg ctggagcggc
     1141 ggtgcggtgg atcgtctttc cacacgcctg caggatgcgg gtttcgaaat gtcgcttagc
     1201 ctaaaagcca aatggcgacc ggaccaggac gctctggagc tatgtcgtga acacggtcgc
     1261 gaaatcgccc gtcagtgggc gctcgcgccg ctgccgcaga gcacggtgaa tacggtggtt
     1321 aaagaagaaa cctctgccac cacgacggct gacctcggcc cacggatgca gtgcagcgtc
     1381 tgccagtgga tttacgatcc ggcaaaaggc gagccaatgc aggacgttgc gccaggaacg
     1441 ccgtggagtg aagttccgga taacttcctg tgcccggaat gctcccttgg caaagacgtc
     1501 tttgacgaac tggcatcgga ggcaaaatga gcaacggcat tgtgatcatt ggttcgggct
     1561 tcgccgcccg ccaactggtg aaaaatattc gcaaacagga cgcctgtatt ccattaaccc
     1621 tgattgccgc cgacagcatg gatgagtaca acaaaccgga cctcagccat gttatcagcc
     1681 agaggcaacg tgccgatgac cttacccgcc agacggcggg tgaatttgcc gagcagttta
     1741 atctgcacct gtttccgcac acctgggtaa cggatatcga tgccgaagcc catgtggtga
     1801 aaagtcagaa taatcagtgg caatacgaca agttagtgct ggcaaccggt gccagcgcct
     1861 ttgtcccgcc agtgcccggg cgtgagttaa tgctgacgtt aaatagtcag caagagtatc
     1921 gcgcctgtga aacgcaactg cggaatgccc gacgcgtgtt gattgttggc ggtggcttga
     1981 ttggtagcga actggcgatg gatttttgtc gtgcaggcaa agcggtcacg ctaatcgaca
     2041 acgctgccag tattctggca gcgttaatgc caccggaagt aagcagccgc ttgcagcatc
     2101 ggttgacgga gatgggcgtt catctgctgt taaaatctca gttgcagggg ctggaaaaaa
     2161 cggattctgg cattctggca acgctggacc gccagcgcag catcgaagtg gatgcggtaa
     2221 ttgccgccac cggactgcgc ccggaaaccg ccctggcacg acgcgccggg ctgacgatta
     2281 atcgcggcgt ttgcgtcgat agttatctgc aaaccagtaa tgccgatatt tatgcgctgg
     2341 gcgattgcgc ggaaattaac ggtcaggtat tgccgttcct ccagccgatt caacttagcg
     2401 cgatggtgct ggcaaaaaat cttctcggca ataacacgcc gctgaaactc ccggcgatgc
     2461 tggtgaaaat caaaacgccg gaattaccgc tgcatctggc aggcgaaacc cagcgtcagg
     2521 atttacgctg gcaaattaat accgaacgcc agggaatggt ggcgcgcggt gttgatgatg
     2581 ccgaccagct tcgcgccttt gtggtcagtg aggatcggat gaaagaggcg tttggattgt
     2641 tgaaaacgtt gccggtgtag gcgagctaca gtgccccaaa tgtcggatgc ggcgctgacg
     2701 cgtcttatcc gacctacagg gaacgcatgt gttgatcaca taatacgttt atgtcgcatc
     2761 ctgcactgtc ccaaaatgtc ggatgcgacg ctggcgcgtc ttatccgacc tacggggacg
     2821 cacgtgtagg ccggataagg cgtttacgcc gcatccggca atggtgtcca aatgcaacac
     2881 gctttatccg ttctggactt cacccgctaa ccagcgcgcc gcagcaataa ccccctgccc
     2941 cagagacaat ccaccatcgc ccgccggtaa actctgcggg aagagcaacg taaaatcagc
     3001 aagataatgc gccagacgtg cacgcagcaa acggttatga ataactccgc cgctaaatac
     3061 cagcgtagtg ataccccgca tcgtggcctg ctcacgcatc aacgcggcaa aaccctgcgc
     3121 cagcgcatca tgaaacgccc acgcgcgttg attaaccgga gcctgccagc tcagccactg
     3181 ctgccagaaa gtggcgagat ccagttgatt gtccaccagc ggcatcgtca ccggatgcgt
     3241 cactccgtcg cacgaagctg cgagcgcctc cagagcacaa gccgcttcac cttcataact
     3301 taacgtggct ggcgcacagc ccagtcccgc cgccacagca tcgaacaaac gcccacacga
     3361 tgacgccagc ggcgcgttaa ttccacgctc aatggcccgc gccagcacgc tccagttttg
     3421 ctgttgcaca cttgccgttt cagggtaatt ctgccactcc ggcacaaagc gcaggcactg
     3481 cgccagcagg ttacgccacg gctgcttcgc cgccaaatcg cccccagcaa gcgccactgc
     3541 aggcaagccg cccaggtgct ggcattcgcg atagttcacc cgcaggcact cgccgcccca
     3601 caaagcgccg ttctccccca taccgatacc gtcgagcgtc aacgcaatga catcaccgcc
     3661 atccagtggc cagtgatgtt ctgccagaca cgccgccgca tgagcatgat gatgcagcac
     3721 cgtttgcgtc ggcagattca tctcacgcgc ccactggctg gagacatagc ccggatgcgc
     3781 gtcatgcaca acgtattgcg gggtgaaatc gtagatgttt tgcatcaggc gtaacgcttc
     3841 gcgccactgc atctggatgc catcgtcact taaatcgccc agatgctggc tcaacaccgc
     3901 ttgttcaccg cgcaccaggc agaaggtatt tttcagatcc gcgccgagac acagcacagg
     3961 cggaacattt ttaaagcccg gaggcaaagc cagcgcatcc ggcacatacc cccgcgaacg
     4021 gcgcagcatt tcgccgcttt cgcgcaccac cgaatcatcc atccgctgca cgatgtcgcg
     4081 gttatgtatc aagaatccgt cggcaatgcc ctgcaaatcc gccagcgcct gttcgttgct
     4141 gatagccggt ggtttaccgc tcaggttgcc ggaggtcatc accagcgggc attgcagttc
     4201 ctgtaacagc aaatgctgga gcgggttcgc aggcaacatc accccaactt cgttaaggcc
     4261 aggggcgata tcatcacaaa gctcaggaac gtatttttta tccaccagca caatcggcgc
     4321 ggcgggtgtg gtaagcaact gacgggcagc gtctggtaaa ccttctgcca ctggcaacat
     4381 aaccgccagc ggtttcgccg ggcgatgttt gcgcgcccgc agtgtcgcca ccgcgttact
     4441 gttacgcgca tcgcaggcga gatgaaatcc gccaatccct ttgatggcga caatgttgcc
     4501 catttttaac tgtgcgatag ctgcctgtaa tgccgcctct tgttctgcat gttcaccatg
     4561 acttacccat tcaagatggg ggccacactc cgggcaggcc accggctggg cgtggaagcg
     4621 acgatcgagc gggtcacggt actctttgtc acaggccgga cacagcggaa acgccgccat
     4681 cacggtaaac gggcggtcgt aaggcatggc gcgaataatg gtgaaacgcg gaccacagtg
     4741 agtacagttg ataaacggat aacgccagcg tcgttcgcct ggggtattca tttcggcaag
     4801 acaagcaggg caagtcgcgg catccgggac aatttgcgta ttcatgacgc cacccgcgct
     4861 ctggcggatg gtgaactcag tgggcagttg tgaccagata aacggctcac gctcgacgct
     4921 atcaatacgc gccagtggcg ggcagtgctg atgcaactgc acaagaaacg tttccggatc
     4981 ttccagcagc cggacttcta cgccatcgcc gtcattacag acatcgccgt gaagatttaa
     5041 ttgctgtgcc agttgccaga caaacggacg aaaaccgacg ccctgcactt tgccacgaat
     5101 acgcagttgg acaccgcaag atgtgttttt tgccattgag ttattcccgc catcatgaat
     5161 tgcgtaaccc gcccagccgg acacgacagc gtcgcatccg gcagtcacag gtcggcgata
     5221 ctgcccgctt cgtattctac gaatatttcc gggaattcct ttgatgccag aacagttcag
     5281 taagattttt agaacatcag cgccgtacgg cgacgttttt ctgcgctcag ttgttcaagt
     5341 ttattacgat cgacacaaat cagcgcatgg gtcgggcaag ccgccataca cgccgggccg
     5401 tcttcacgat ggttgcacag gtcgcattta ttggcttcgg ctttgtcagc ccgtacattc
     5461 agccccgcgc cgctgttgcg aatcaccgga cgtaccacca cttccatcgc gccatacggg
     5521 caagccacaa cgcaggtttt gcaaccaata caacgttcct gcatcacatg aacaaaccct
     5581 ttatcacgac tgatagcacc attcggacag acgttagcgc acggtgcatc ttcacactga
     5641 cggcaaactg tcgccgtgga aatgttcaca cctttaatga catggattcg cggtaaaaaa
     5701 gtttccgggg tcagcgatgc acagtcctga ttttcctgat gagaaaccac gcacgccact
     5761 tcacaggtac ggcaaccaat acatttactc gcgtcagcaa tgatgaaacg gttcatcaaa
     5821 ttctccagca atgacagtta atgcgccgat acattcacaa atcatgccag tttttaattt
     5881 actgttattt aaagaaatta aattctgtaa tgcaggaaaa acgatgtcat cgacactagt
     5941 gacgatgaca tgtgatgaca atgtttatcg cgaaagagca atgagggagt cgcggcggat
     6001 cagttttccg ctgaaggttt tcggcggtgt gtaagcacca ccatcgagca taaatatcag
     6061 ccgcccaatt atttcctgaa tcatctcagt caccggaatt tttacgctgg agagcgccgg
     6121 aacggtgtag ggggcaatag cgatatcatc gaatccgata actgacacct gctctggcac
     6181 cgctacgccg cgctcgtgta acgctttcat cgcacctatc gccatatcgt cgttactggc
     6241 aactaacgcg ctaaatttag ccccacgttc gagcaacatt tctacccctt cggccccgct
     6301 ggcaggcgtc catttaccgt tagcgataag tttttcattg agcgcaatac catgctgcgc
     6361 cagcgcgtct ttatacccgg caagacgttc aacgctggtg ggggaatcca tcgaaccggt
     6421 aaggaaagca atctcctgat gtccggcgtt tatcaactct gccacggcgt taaagctggt
     6481 ctgtttatga tcgcaccaga cgctatggct gctgtttttg cgcaggcggc gattaagcac
     6541 cattatcggc tgactgtgtg cgtcaatgat gtcatcaatc tcatccacgc ttaaaaaacg
     6601 cgggtaaatc ataatggcgt cgcagcgcaa atccagcaga tactgaatcg cctggcgctc
     6661 ttcttcggcg ctgtgtttac catctgccaa tagcaactgt cgccctttct cttccgccat
     6721 tcgcgcggca tgaaagagta attcactaaa ataaatgccg tggtaaagcg tgttggtcac
     6781 gaccagcccc agcgtctgag tactcttcgc cgacagattg cgcgccagca agtttggacg
     6841 gtaaccgctc tcttctaccg cctgaaacac gcgatcttta gtctcctggc tgacgtagcc
     6901 attacctgaa agcacgcggg aaacggtcgc ttttgaaacc ccggcgcgct tcgccacttc
     6961 cagcatcgtc gtcatcattt tcatcccttt acacgcaatc aacgcagtgt actgcaccgt
     7021 ttgccgattg tccttgcgca atcggcggga aaaatattca ggtgaccggt ttcacaaata
     7081 taaaaaatga acaattaact ctcttgctta ttcagtgaca actattcatg attttgtgaa
     7141 accggtttcc taattccgtt tcagcatcgg catttttccg tcacgtcgac tgataacaac
     7201 tacatctacc ctactgataa caggataaaa tccgatggcc aaaaattatg cggcgctggc
     7261 acgctcggtg atagcggcac tgggcggcgt tgataacatc tcggcagtca cgcactgtat
     7321 gacgcgtttg cgctttgtta tcaaagatga ccagcttatc gacagtccga cgttgaaaac
     7381 catctccggc gtgctcggcg tggtacgtag tgacaaccag tgtcaggtga ttatcggcaa
     7441 taccgtttca caagcctttc aggaagtcgt cagcctgctg ccgggagata tgcagcccgc
     7501 acagcccgtg ggtaaaccca aacttacgct acgtcgcata ggtgcgggga tcctcgatgc
     7561 gctgatcggc accatgtcac cgctgatccc ggcgattatc ggcggatcga tggtcaaact
     7621 gctggcgatg atcctcgaga tgagcggcgt gctgacaaaa ggatcgccga ccttaaccat
     7681 tctgaatgtg attggtgacg gtgctttctt cttcctgccg ctgatggtcg cggcttcggc
     7741 cgccatcaaa tttaaaacca atatgtcgct ggcgattgcc atcgcgggtg ttctggtaca
     7801 tccgagtttt attgaactga tggcgaaagc ggcccagggt gaacatgtcg aatttgccct
     7861 gatcccggtc accgcggtga aatacaccta cacggtgatc ccggcgctgg tgatgacctg
     7921 gtgcctgtca tatatcgaac gctgggtgga tcgcattaca ccggcggtga ctaaaaactt
     7981 cctcaagcca atgctgattg tgttgattgc cgcaccgctg gcaatcctgc tgattggccc
     8041 gattggtatc tggatcggta gcgccatttc ggcgctggtt tacaccattc atggttatct
     8101 gggctggctt tcagtcgcca ttatgggcgc gctgtggcct ctgctggtaa tgaccgggat
     8161 gcaccgcgtc tttacgccaa ccatcattca gaccattgcc gaaaccggca aagaagggat
     8221 ggtcatgccg tcagagatcg gcgctaacct gtcgctgggc ggttcatcac tggcagtggc
     8281 gtggaaaacg aaaaacccgg aactgcgtca gacggcgctg gctgcggcgg catcagccat
     8341 tatggcgggg atttccgaac cggcgttata tggcgtggcg atccgcctga aacgtccgct
     8401 tatcgccagt cttatcagcg gttttatttg cggcgcggtt gccggtatgg cggggcttgc
     8461 cagccactca atggcagcgc cggggctatt taccagcgtg cagttcttcg atccggcgaa
     8521 tccaatgagc atcgtctggg tgttcgccgt catggcgctg gcggtcgtgc tgtcgttcat
     8581 cctcacactg ttgctcggct ttgaggatat tcctgttgag gaagcgactg ccgaggcgcg
     8641 aaagcatcag agcgcacaac cgaccgtcgc caaagaagta agtcttaatt gaggatgaaa
     8701 atgtcagtat ttccagaagg ttttttatgg ggcggcgcgc ttgccgccaa ccagtctgaa
     8761 ggtgcgtacc gggaaggtgg caaagggctg accaccgtcg atatgatccc acacggcgag
     8821 catcgaatgg cggtgaaact ggggctggaa aaacgttttc agttgcgcga tgacgagttt
     8881 tatcccagcc atgaggcgac ggatttttat catcgttata aagaagatat cgccctgatg
     8941 gcagagatgg gattcaaagt gttccgtacc tcaattgcct ggagccgcct cttcccgcag
     9001 ggcgatgaac tgacgcccaa ccagcagggc attgcttttt accgttctgt ctttgaagag
     9061 tgtaaaaagt acggtatcga accgctggtc acgttgtgcc acttcgatgt gccgatgcat
     9121 ctggtcaccg aatatggctc ctggcgtaac cgcaagctgg tggagttttt cagccgctac
     9181 gcccggacct gctttgaagc atttgatggt ctggtgaaat actggctcac cttcaatgaa
     9241 atcaacatta tgttgcatag cccgttctcc ggcgcgggtc tggtgtttga agaaggcgaa
     9301 aatcaggatc aggtgaaata tcaggctgcg catcaccaac tgattgccag tgcattagcc
     9361 accaaaatcg cccatgaggt taacccgcaa aatcaggtgg gctgtatgct ggcgggcggt
     9421 aacttctacc cttacagttg caagccggaa gatgtctggg cggcgctgga gaaagatcgg
     9481 gaaaacctgt tttttatcga tgtgcaggcg cggggcgcgt atccggctta ctctgcccgc
     9541 gtattccgcg aaaaaggggt aaccatcgac aaagcaccgg gcgatgatga gatcctgaaa
     9601 aacaccgtcg attttgtctc tttcagctat tacgcctcac gctgcgcctc ggcggagatg
     9661 aacgccaaca acagcagtgc ggcgaacgtg gtgaaatcgc tgcgtaaccc atatctacag
     9721 gtaagcgact ggggctgggg gattgatcca ctcggtctgc gtatcaccat gaacatgatg
     9781 tatgaccgct atcagaagcc gctgtttctg gtggaaaacg gcctcggcgc aaaagatgaa
     9841 cttgctgcca atggcgagat taacgatgac tatcgcatca gctatttacg cgaacatatc
     9901 cgcgcaatgg gcgaagcgat tgcagatggc attccgctga tgggctacac cacttggggc
     9961 tgtattgatt tggtttccgc ctcaacgggt gaaatgagca aacgctacgg ttttgtctac
    10021 gtagaccgtg acgacgcagg caacggtacg ctggcgcgca cgcgtaagaa atcgttctgg
    10081 tggtataaaa aagtgattgc cagtaatggt gaggatttag agtaagtaac agtgccggat
    10141 gcggcgtgaa cgccttatcc ggcctacatg tcgcacgaac caggtaggcc cgataagcgc
    10201 agcgcatcgg gcaatctggc gtttgctatc agttctgatt ccctcatcaa gaacatccct
    10261 gtcctgattc cttactgaaa aactactctt cttccaccgc taactgcgcg aatccgccat
    10321 tcccttccca gccttccagt cgttgataaa cggtttctac cgcatcttta atcggctggg
    10381 tcatcgggta gtaaaagccg acgatatccg gctggatgcc gaggaaaatc acttcgccaa
    10441 tgtcttcttt taactggtcg ataaggtagt tcaacggcat gttatgggta gtcatcataa
    10501 acatctcggc gatatcatcc ggatcgatga tgcggatctc gccggggttt aaccccatat
    10561 ccgtggcgtc gacaatcagc agccgtgtcg ggcgcagttc gcggatggcg acgatgtcgt
    10621 tttccggagc gctaccgccg tcaatcacca cccagttacc tttcggcgcg gcggcgcact
    10681 tttccgccag cagcgggcct gcgccatcat cgcccatcat gctattgcca acacaaagta
    10741 aaacgtcagt cacgcagtct cctcaccatc aaatagatgg cgttttcctg atgaatatca
    10801 tgcagcatac ttaacagcat tgtactccat tctttctgct gcgaagtttg cgctgcccgg
    10861 gctttatcga atgcgttagc cagcatcggc acatggttaa tgtcgatgac gatctcacca
    10921 tatttcggca cgccttccat tttgcggcgg gcttcgctgc ccgcctccag agtggcaatc
    10981 catgccagat agtcatccca cgggcaggta agcgccgctt ccaggcaatc gataacccca
    11041 aggtggtgac caatcgccag gctgtaatag accacctgct gcgcctcggc gggcgtagca
    11101 tcgttctcat caataaattt acggctcagt tgactgaaca ccaccttttc actcatcgga
    11161 tacgcgcctc ttcaacaaca tgattcagat ggctgacaat ctcgttcaga cgcggatcgt
    11221 tttccgcttc cagccagcgt gcaacctgtt cttcgccctg ccctaactgt gtaaggtaat
    11281 catcggcaat ctgacgaccg taacgataac ccgccaggcg gcgtgcttcg cgatccactt
    11341 tcacacgcag cggctgcacc atatcgccat gcaggatctc cgccggttgt tcatcctgct
    11401 cacccggccc acgggcgtga attttctgct ccagcaggcc gagcgccatt gcaaagccgt
    11461 atagcgtagc ggcaggcgtt ggcgggcagc cagggatgta gacatctacc gggacgattt
    11521 tatccgtacc gccccacacg cagtagagat cgtggaagat cccgccactg ttaccgcagg
    11581 caccgtagga gatacagatt ttcgggtccg gcgcggactg ccacgcacgc agcgcagggg
    11641 atcgcattgc acgggtgacc gcgccggtaa acagtaaaat atccgcatga cgcggtgaag
    11701 gaacgacttt aatgccgaag cgttctgcat caaacagcgg cgaaagcgtg gcgaaaattt
    11761 cgatttcgca gccgttgcag ccaccgcagt ccacgcggta aacgtaggca gaacgtttga
    11821 tttttttcag taacgacgcc ttcatgctgg cgatggattc atccaccgtc atcgggaccg
    11881 gaatgccgtt ggcgtcacgg gggcctaata aattgctcat cagatggcct ctttcatatg
    11941 gcgagtcagt tcaatacggt cggacggcac caggcatttc tggcgcttac attccgggca
    12001 agtctcaaag ctttcgcggt ggttttccgc gcggctgtca ccattgtgct taagcagcgc
    12061 aatggcgtag tcgatctctt tctggacggc gaaaggacga ttgcagacgc ggcagttgca
    12121 cagcgcgaag cgggactgtt ggaggaagtc ttctttcttc cacaccgcca gttcgtactc
    12181 ttgcgacagt ttgatcgccg ccgtcgggca aacttcttcg cagcgtccac agaagatgca
    12241 gcgcccgaga ttaaactccc aggccagctc tcctgtggcg aggtcggttt caaccgttaa
    12301 ggcgtttgac gggcaggcat tgacgcaggc cgcgcagccg atgcactgct gcgggttctg
    12361 ctctggctta ccacggaagt ttttatcaac cgcaatcggc tccagcggat aagacgaggt
    12421 cgccgtgccg gttttgatga cttttttgat aaaggtaaac atggcgattc cttatttcag
    12481 cggcgagttt ttacgctcaa tgctgtaacg ctcgagttct ttgtacggca ccactttgct
    12541 cttcttctta cgcacatcga ccacggtcat gcggtcggta caggagtagc aagggtccag
    12601 gctaccgata atcagcggcg catcggaaac ggtgttgccg cgcagcatgt agcgcagggt
    12661 cggccagttc gcgtaggtcg cggcacggca gcgccagcgg tacagcttct ggttgtcgcc
    12721 ggtcatgctc cagtggatat cgtcgccgcg cggcgcttcg gcaaagccca gcgcaaaacg
    12781 gtgcggaatg taggtaaaac cttctaccat caacggaccg cccggcaggt tatccagacc
    12841 gtagtcgatc atgttcagcg cggtatagac ctcgttgatt cgcactttca gacgggaaat
    12901 aacgtcgcag ccctgctcgc tgtggacttc cattggcagc aggccataac cgacaaacgg
    12961 gtgatcggcg cgagtatcgc gggcgtgacc gctggcgcga accatcgggc caacgttact
    13021 gaaatcgcgg gcgatttccg ggtccagacg accaatgccg acggtgcgct gttccatgtt
    13081 cggtgtgctc agcagcacat ccaccagctc ctgcacttca cgacgcatct gttgtgccag
    13141 ctggcgggtc tggatcatgt cgtctttcag cagatcgcga cgaatcccgc cgatcaggtt
    13201 caggccgtag gttttacgtg caccggtaag gatctctgcc attttcatgg aggtttcacg
    13261 cacgcggaag aactgcataa agccggagtc gaagccggta aagtgacagg cgaggccgag
    13321 attgagcaga tgcgagtgca ggcgttccac ctccaacaga atggcgcgga tcatctgcgc
    13381 acgttctggc acctgaatac ccatcgcgtt ttccaccgac gtggtgtagg cggtgctgtg
    13441 agcaaagccg cagatcccgc acacacggtc agagaggaag gtcacttcgt tgtaacccat
    13501 acgggtttcc gccagttttt ccatgccgcg atggacgtag aacaggcggt agtcggcgtc
    13561 gataatgttt tcaccatcga caaacagacg gaagtgaccc ggttcgtcag aagtgacgtg
    13621 caggggacca atcggcacga cgttgttttt cttgtcgccc agttcgttga tgaactcgta
    13681 ggtttcagca tcggtagtcg gtgccggacg ctgacgataa tccatgctgt ctttacgcag
    13741 aggataaagt tcatccggcc agtcatccgg cagcaccaga cggcgttcat ccggcagacc
    13801 aaccggaatc aaaccgtaca tatcgcgcac ttcacgctcg ccccacactg ccgccggaac
    13861 gcgcggcgtc acggaaggat actccggttt gttggcgtca acttcgacgc gcaccgttac
    13921 ccaacactta gtccccttct ccatcgacag cacgtaataa acggcgtaat gaccattcag
    13981 tttgcgttcg tcgttaccaa acagcaccga cagccagccc ccctgcttgt agtagagaaa
    14041 ctccaccact tccggcaggt agttcacctt cacggtcacg gtcagctgat ctttggtctg
    14101 ccaggcgtgg tccagcacga cgcccggaaa tgcctcattc agcgcggcga gataatgttg
    14161 acctaatttt tcttcagaca tgctcaaact ctctttaatc acgccgccag caaggagacg
    14221 aacgctaaaa atgcaaagcc aaaaccagcc caggtaatgc gtggagtaat atcaagacgc
    14281 aaacgggcca tgctgttttc gaacagcgcg ataaccagta cgccgaccac cagtttgacg
    14341 atggcaatca ccagcgccag cagcagccca ccgacggtga aggtttccat ttgtccccac
    14401 ggaataaaca ccccgacgaa catctgcaac accaccagct gtttcaggct gatgccccat
    14461 ttcatgacgc caaagccgct gccgctatac tcagagagcg gaccttcctg caactcctgc
    14521 tcggcttccg ccaggtcgaa cggcagtttg cccatttcga taaaagtggc gaacgcacag
    14581 gcacaaagcg ccagcaccag cgggatgctc tggctcagtg gccagtgata aacggtgtcg
    14641 gtgatgttgc tgatgttggt ggaaccggca acctgtgcgg cgacccacag accaagcagc
    14701 agcatcggtt caaccaacac gccaagcatc gcttcacggc tcgcgccgat agcggtaaac
    14761 gggctaccag tatccagacc agaaatggca aagaagaaac gcgcgatggc gaagagatac
    14821 agtaaggtga tcaaatcgcc cagttgcggc agcggagagc cgacggtcac caccggcagc
    14881 gcagtggcga tagtcagcat gacgcccacc atcacatacg gcgtcaggcg gaacacccag
    14941 ccggaggcat ccgggccaac gctctgacgc cccagcagtt tgataatgtc gcgatactcc
    15001 tgcaacacgc ccggcccacg acggttatgc aagcgggcgc gcgctacgcg ggtaataccg
    15061 gagagcagcg gcgcaacggc aaataacacc agcgcctgaa ttaacggata taaaacactc
    15121 attctcaggc tcctcgtgaa acaataatca ccaccagtac cgccagttca accagcgcca
    15181 tccggcggaa cagcaacgca ctcccctcgc actgccagcc cggcaccagc gacaccggat
    15241 tcagccattt gcgtagtttc agcaccggcg caaacgcctg tttcaccggc atggcaaaac
    15301 cgtgagcggt aatcaccatt gatttttcat gatcgtagcc gcacacccag gccgcgccgc
    15361 gggaacgcga cggcaaacga tcgcctttgc aaatcgccat aatgatgaat ggcagcagcg
    15421 ggcaggcaat cagcagcaac gtgatcatgg gttgagaaac ggtggtgtta gcaggctcca
    15481 gcggcagagg tacagcagca gagagcatcg gcagtagcca cggcgcagca acaccgccaa
    15541 ttacgcagca aatcgccagt gccactacgc ttacgctcat caggagcggc gcacaggtgg
    15601 cgttttcggc ctctttggta cgcggcgcgc cgaggaaagt aacgccataa actttcgcca
    15661 tacacatcac cgccagcgca ccggtaattg ccagccccac ggcgagcagc ggcccgagaa
    15721 gacgggcaac aaacgcgcca ctattgctca gtttgaagaa ggattgatag ataacccatt
    15781 ccccggcaaa cccgttcagc ggcggcagcg cagccattgc catcagcccg actaacatgg
    15841 cgatggagat aaccggcatt ttcttgccaa taccaccgag tttttcgata tcgcgatgac
    15901 cggtacggaa ccagacgctc cccgcaccaa ggaacagtac gctcttgaac aggctatggt
    15961 taagcagatg gtacagacca ccaaccagac caagggcaat cagcgccggt tgttcgagcg
    16021 cgatacccgt tacgccagcg cccagcccca gcaggatgat gccgatattt tccagggtgt
    16081 ggtaagccag cagacgttgg atattatgtt ccatcaacgc atacagaccg ccgacgaatg
    16141 cggtgatcat gccgagtacc agcagtgcga tcccccacca cagcggtgca ttaccgccca
    16201 gtagtgacag agttaaaatg cccagcaggc caattttcat gactaccgtg gaaaacagcg
    16261 cggcagctgg cgcagaggcg ttggcatgtg cctgtggcac ccagccgtgc agcggaataa
    16321 tcccggccag caggccaaag ccaatcacac cgagcagcca gatatcggaa ccgagcggca
    16381 gctgttgcat acgcatatcc agcaggcgca gatccagcgt gccgtaacgc tgccacagca
    16441 gccagcaggc aatcgccagc agcagagtgc caagacgccc cagcgcaaac cacagtttgc
    16501 cctctttgct gttgctggtg aggaacaccg cacacagggc catgatttcg gccattacca
    16561 cgaacatgcc gaggttgctg gcgatgacgg cgcagacggc ggcagccatc aacatattga
    16621 tctgcaagcc gttgcacttc acctgcgcgt ggcgatgcca gtcaatgttg tagaggctga
    16681 caaacagacc gcataggccg agcgtaatca gccaaatcgc attaagcgga gagatttgca
    16741 catcgtagct taccagcgac agcgcaccgc tcacgccaac cgcgccagtc agcactgtga
    16801 agcccgcggc tgccgtatac aggctaccaa ccgcgccgcc aattccggct atccagccgc
    16861 ttagcgcctt ttgaaaagaa aagagaaatg ccagaacagc ggcggcgaca aaccacgcca
    16921 cgccgctatt gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa
    16981 caaggtgaga tcgccaaaat ccgtgttaaa agtcagctca cgcttacgtt tgctgacgcg
    17041 ggcgatatcg gtgttatcca ccagatgcag ggctttagtc gggcacatcc gcacgcaggc
    17101 cggaccttgt tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc gaatacctgg
    17161 cacccagtca agcaatgtgc tgacacgcgc cggagcaggc ggtgccggtg gcgctttcgg
    17221 ggtattggcg tttgccggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa
    17281 cgggcaggcg atgccgcaca gtttgcagct tacgcacaga ctttcattca actgcacggc
    17341 cccatcaacg cgggtgatgg cgttaaccgg gcaaaccacc gcacagggcg catcttcaca
    17401 gtggtgacag agctgcggcg cagattcttt ttcattgagc atcactcgca ggcgcggcat
    17461 tgattgcagg ccgtgctggc gatgcgtctc tgaacaggcg gcctcacagg tgtggcagcc
    17521 gatacagagc gtggagtcag caattacaaa acgattcacc aggcattcct caggtgattg
    17581 tcatttttga cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat
    17641 ccccaaacag gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt
    17701 gtccctttac cagttggctt aaattcaccg gcacattgtt ttcaatggcg gtgtataagc
    17761 tgtcgtaaac gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga
    17821 tgttgacctt gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca atccgctgtt
    17881 tgtcttcacc atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg
    17941 ccagggtgac gtaaatacga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg
    18001 cgcagctcat ggcgtgatgc aggcgcgctt tcgataacgt gattccctga accagcgaat
    18061 tgcagtagat gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg taatcggctt
    18121 tctcgcttat ttcccaaata gtcattgtca ggttacccgt ttaacagaga tgccagcttt
    18181 aagcattttt tgtgccaact tttaatttat tgttattaaa gcgattttta agctaaagat
    18241 gaatttcgtc gccgtgtcga cgtgtcattt cgacatcatc gacattattc accgcaggga
    18301 taatcaacac tggcacaatt attgcttgta gctggcaata gttaatggga ggcgatatgc
    18361 acgaaataac cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg
    18421 gcgcaaaacg cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca
    18481 gctctcttgc cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac
    18541 tgcacctcga agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc
    18601 tactgaccca gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta cagattgtgg
    18661 cagacgacgg tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa
    18721 catgcggttg cggtgaaggc aacctctata ttgagggtga tgaacataac cctcattccg
    18781 cgtttcgtag cgcgccattt gccccggcgg cacgcccgaa gatgaaaatc accggcatta
    18841 aagcgcctga atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg
    18901 aagcgggcac tcacgcgccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg
    18961 tgctggacaa aaataaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc
    19021 aactggtgct caacctggtt tccagccctg gttccggtaa aaccaccctg ctgacggaaa
    19081 ctttaatgcg cctgaaagac agcgttccgt gcgcggttat tgaaggtgac cagcaaaccg
    19141 tgaacgatgc cgcacgcatt cgcgctaccg gcaccccagc gattcaggtg aacaccggta
    19201 aaggctgcca tcttgacgca cagatgattg ccgacgccgc gccgcgcctg ccactggacg
    19261 ataacggtat tctgtttatc gaaaacgtcg gtaacctcgt ttgcccggcc agcttcgatc
    19321 tcggtgaaaa acacaaagtg gcggtgcttt ccgttaccga aggtgaagac aaaccgctga
    19381 aatatccgca catgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacttgctgc
    19441 cgtatctcaa tttcgacgtt gagaagtgca tcgcctgcgc ccgtgaagtc aatccagaaa
    19501 ttgaaatcat ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc
    19561 tggagacaca gcgatgtgca taggcgttcc cggccagatc cgcactattg acggcaacca
    19621 ggcgaaagtc gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg
    19681 cgatgaaaac ggtcagccac gcgtgggcca gtgggtactg gttcacgttg gctttgccat
    19741 gagcgtaatt aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga
    19801 cgttgagcca gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat
    19861 gaatatcgcg cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca
    19921 catctctctt acaccgccga acgccctctg cggattatgg aagtgtgtgg cggtcatacc
    19981 cacgccattt ttaaattcgg cctcgaccag ttgctaccgg aaaacgttga gtttatccac
    20041 ggtccgggtt gcccggtgtg cgtactgcca atgggtagaa tcgacacctg cgtggagatt
    20101 gccagccatc cggaagtcat cttctgtacc tttggcgatg ccatgcgcgt accagggaaa
    20161 caggggtcgc tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgcca
    20221 atggatgcgt taaaactggc gcaggagaat ccaacccgta aagtggtatt cttcggctta
    20281 ggttttgaaa ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa agcccgcgat
    20341 gtgcagaatt tttacttctt ctgccagcac attacgctta tcccgacgct gcgcagtttg
    20401 ctggaacagc cagataacgg tatcgacgcg ttcctcgcgc cgggccacgt cagtatggtt
    20461 atcggcactg atgcctataa ttttatcgcc agcgattttc atcgcccgct ggtggtcgct
    20521 ggtttcgaac cccttgatct actgcaaggc gtggtgatgc tggtggagca gaaaatagcg
    20581 gcccacagca aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg
    20641 gcgcaacagg cgattgccga tgtgttctgt gtcaacggcg acagtgaatg gcgcggctta
    20701 ggcgtgattg aatcttctgg cgtgcacctg acgccggatt atcaacgatt cgatgccgaa
    20761 acacatttcc gcccggcacc gcagcaggtc tgcgatgacc cgcgtgcgcg ttgtggcgaa
    20821 gtcttgacgg gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct
    20881 caaaccgcgt ttggtgcgct gatggtttcc tccgaaggag cgtgcgccgc gtggtatcag
    20941 tatcgtcagc aggagagtga agcgtgaata atatccaact cgcccacggt agcggcggcc
    21001 aggcgatgca gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg
    21061 cagagcagga agatcaggcc cgtcttgatt tggcacagct ggtagcggaa ggcgaccgtc
    21121 tggcgttctc aaccgacagt tacgtcattg acccgctgtt ctttcctggc ggtaatatcg
    21181 gcaaactggc gatttgcggc accgccaatg acgttgcggt cagtggcgca attccgcgct
    21241 atctctcctg tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag
    21301 tgaccagcat ggcagaaacc gcccgcgcgg caggcattgc catcgttact ggcgatacca
    21361 aagtggtaca gcgcggcgcg gcagataaac tgtttatcaa caccgctggc atgggcgcaa
    21421 ttccggcgaa tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttgctggtta
    21481 gcggtacact cggcgaccat ggggcgacta tccttaacct gcgtgagcaa ctagggctgg
    21541 atggagaact ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg ctgcgtgaca
    21601 ttcccggcgt gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg gtggttcatg
    21661 agttcgcggc agcctgcggt tgtggtattg aactttcaga agcggcactg ccggttaaac
    21721 ctgctgtgcg cggcgtttgc gaactgctgg gactggacgc ccttaacttt gccaacgaag
    21781 gcaaactggt gatcgccgtt gaacgcaacg cagcagagca agtgctggcg gcgttacatt
    21841 cccatccatt gggaaaagat gcggcgctga ttggcgaagt agtagaacgt aaaggtgttc
    21901 gtcttgccgg tctgtatggc gtgaaacgaa ccctcgatct accgcacgcc gaaccgcttc
    21961 cgcgtatatg ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc
    22021 tttgcggtgc tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacag
    22081 caagggttgt tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt
    22141 gaggctcttt cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg
    22201 tggcaagcgc agactcaacg tgcgtcttat tacgcatcgc gtgaaaaaga cacccccatt
    22261 aaatatgaag acgaaactgt tctggcacac ggtccggtac gcagcatttt gtcgcgccct
    22321 gatacgctgc attgcagtta cgaagaattt tgtgaaacct ggccgcagct ggccacaggt
    22381 gggctatacc ccaaatttgg tcactattgc ctgatgccac tggcggcgga agggcatatt
    22441 tttggtggct gtgaatttat tcgtcatgac gatcgcccct ggagcgaaaa agagttcaat
    22501 cgtctgcaaa catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc
    22561 gttaacaatg tcgactatga gttgttatgc cgggaacgcg ataacttccg catcctggtc
    22621 gccatcacta acgcggtgct ttcccgcctg gatatggacg aactggtcag cgaagtcgcc
    22681 aaagaaatcc actactactt cgacattgac gatatcagca tcgtcttacg cagccaccgt
    22741 aaaaacaaac tcaacatcta ctccactcac tatcttgata aacagcatcc cgctcacgaa
    22801 cagagcgaag tcgatgaagc cggaaccctc accgaacgcg tcttcaaaag taaagagatg
    22861 ctgttgatta atctccacga gcgggacgat ttagccccct atgaacgcat gttgttcaac
    22921 acctggggca accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg
    22981 ctgggcgtgc taaaactggc gcaatgcgaa gagaaagtct ttaccactac caatctgagc
    23041 ttactgcgcc agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag
    23101 gaaattcatc gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc
    23161 aacaatgttg atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagcgta
    23221 cttaaacaag tggaaatggt ggcgcaaagt gacagtaccg ttctgatcct cggtgaaact
    23281 ggtacaggta aagagctgat tgcccgcgcg atccataatc tcagtgggcg taataatcgc
    23341 cgtatggtca aaatgaactg cgcggcgatg cctgccggat tgctggaaag cgatctattt
    23401 ggtcatgagc gtggggcttt taccggtgcc agcgctcagc gcattggtcg ttttgaactg
    23461 gcggataaaa gctcactatt cctcgatgaa gtgggcgata tgccgctgga gttacagccg
    23521 aagttgctgc gtgtattaca ggaacaggag tttgaacgtc tcggcagcaa caaaatcatt
    23581 cagacggacg tacgtttaat tgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac
    23641 cgagagttcc gtagcgatct ctattaccgt ctgaacgtat tcccgatcca cctgccgcca
    23701 ctacgcgagc gtccggaaga tattccgctg ctggcgaaag cctttacctt caaaattgcc
    23761 cgtcgtctgg ggcgcaatat cgacagcatt cctgccgaga cgctgcgcat cctgagcaac
    23821 atggaatggc cgggcaacgt gcgtgagctg gaaaacgtca ttgagcgcgc ggtattgcta
    23881 acacgcggca acgtgctgca gctgtcattg ccagatattg ctttaccaga acctgaaacg
    23941 ccgcctgccg caacggttgt cgcccaggag ggcgaagatg aatatcagtt gattgttcgc
    24001 gtgctgaaag aaacaaacgg cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg
    24061 ctgaaacgca cgactctgct gtcacggatg aagcgactgg gaattgataa atcggcattg
    24121 atttaacaga tgtggttata ctcgatcaac attcaatgaa aggaggcagt atggcagttt
    24181 cagcaagaaa tcaattaacc ggaacagtta gcacagtagc aatgggcgct gtgaatgatg
    24241 aagtagaact gacgctggca ggtggtgcaa agctggtggc aattgtgact cacagcagcc
    24301 agcaagcgct gggtctggcg aaagggaaag aagcgattgc cctgattaaa gccccgtggg
    24361 tcacgctggc aacggaagac tgtggactga aattctctgc tcgcaaccag tttgctggca
    24421 gcgtgtcgac aatcaccgaa ggcgcagtga acgccactgt ccatatcaaa acggatgcgg
    24481 gttttgaaat tgtggcagtg gtaacaaatg aaagtcagga tgaaatgaaa ctcaccactg
    24541 gctcgcgcgt tattgcattg atcaaagcat cagccatcct gattgccacc aaagcgtaaa
    24601 cgccttattt gccggagagt atggctctcc ggcagattta tttctttgga cgatattttt
    24661 ctggcaactc cggcaccgga cgcttgtcat cgatgagatg acgcacggtt aagatcggat
    24721 gacgccacag cattctcggc ccggcccaac gcataatctg tttcatctct tcacgctttg
    24781 caggctggta acagtgcacc ggacactgct tacaggctgg tttctcttca ccgaacacac
    24841 atttatccag ccgcttttgc gcgtaagcaa acaacgcctc gtaatgctcc ggctctgctg
    24901 acgcctgcgg gcatttcgct tgataaagat cgatcatttt tttaatcgtc agtttttcac
    24961 gagagatacg cttgccggac atgctgcctc catactctta aggtgcattt atattacaac
    25021 ttaattttaa aggggactat ggctctgaag gaggaggttt cttcaaccga cgaatttggc
    25081 gatcttgttt catcgtcatc acgcaaaagc tgcaaagcag catttttcgc ggaaccgaca
    25141 tcaagaactc acctttcgcg tcttcccctg aaatgattaa ctccggtatc atgtgcgcct
    25201 tatgtgatta caacgaaaat aaaaaccatc acactacatt taacatcagg gagccggact
    25261 taaccccatg agaacaatag aaaatttcga cgcccatacg cctatgatgc agcagtatct
    25321 caagctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggcg acttttatga
    25381 actgttttat gacgacgcaa aacgggcgtc gcaactgctg gatatttcac tgaccaaacg
    25441 cggtgcttca gcgggagagc caatcccgat ggcggggatt ccctaccatg cggtggaaaa
    25501 ctacctcgcc aaactggtga atcagggcga atcggttgct atttgtgaac agattggcga
    25561 tccggcgacc agcaaagggc cggtcgagcg caaagttgtc cgtatcgtta cgccgggcac
    25621 catcagcgat gaagcgctgt tgcaggaacg tcaggacaac ctgctggcgg ctatctggca
    25681 ggacagcaag gggttcggct acgccacgct ggatatcagc tccgggcgtt ttcgcctgag
    25741 cgaaccggcg gaccgcgaaa cgatggcggc agaactgcaa catacgaatc cagcggaatt
    25801 actgtatgcg gaagatttcg ccgagatgtc gctgattgaa ggtcgccgcg gcctgcgtcg
    25861 tcgcccgctg tgggagtttg aaatcgacac cgctcgccag caattgaacc tgcaatttgg
    25921 cacccgagat ctggtcggtt ttggtgtgga gaacgcacca cgcggacttt gtgctgccgg
    25981 ttgtctgttg cagtatgcga aagataccca acgcacgacc ctgccgcata ttcgttctat
    26041 cactatggaa cgtcagcagg acagcatcat tatggatgcc gcgacgcgtc gtaacctgga
    26101 aattactcag aacctggccg gcggtgcgga aaatacgctg gcttcagtgc tcgactgtac
    26161 tgtaacgccg atgggtagtc gtatgctgaa acgctggctg catatgccag tgcgcgatac
    26221 ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca ccgccgagct
    26281 gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt ctggcgcgtc tggctttacg
    26341 aactgctcgt ccacgcgatc tggcccgtat gcgtcacgct ttccagcaac tgccggagct
    26401 gcgcgcgcaa ctggaaggtg tcgatagcgc cccggtacaa gcactgcgtg agaagatggg
    26461 cgagtttgcc gaactgcgcg acctgctgga gcgagcaatc atcgacacgc cgccggtgct
    26521 ggtacgcgac ggtggtgtta ttgcgactgg ttataacgaa gaactggatg aatggcgcgc
    26581 gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc gtgaacgtac
    26641 tggcctggac accctgaaag tcggctttaa cgcggttcat ggttattaca ttcagatcag
    26701 ccgtggacaa agccatctgg cacccatcaa ttacatgcgt cgccagacgc tgaaaaacgc
    26761 cgagcgctac attattccag agctaaaaga gtacgaagac aaagttctca cttcaaaagg
    26821 caaagcactg gcactggaaa aacagcttta tgaagagttg ttcgacctgc tgttgccgca
    26881 tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc tggtgaacct
    26941 ggcggaacgg gcctatacgc tgaactacac ctgcccgacc tttattgata aaccgggtat
    27001 tcgcatcacc gaaggccgcc atccggtggt tgaacaggtg ctgaatgagc cgtttatcgc
    27061 caacccgctg aacctgtcgc cgcagcgtcg catgttgatc attaccggtc cgaatatggg
    27121 cggtaaaagt acctatatgc gccagaccgc actgattgcg ctgatggcct acatcggcag
    27181 ctatgtaccg gcacaaaaag tcgagattgg acctatcgat cgcatcttta cccgcgtagg
    27241 ggcagcggat gatctggcat ccgggcgttc aacctttatg gtggaaatga ccgaaaccgc
    27301 caatatttta cataacgcca ccgagtacag tctggtgctg atggatgaga ttgggcgtgg
    27361 aacgtccact tacgatggtc tgtcgctggc gtgggcgtgc gcggaaaatc tggcgaataa
    27421 gattaaagcg ttgacgttgt ttgctaccca ctatttcgag ctgacccagt taccggagaa
    27481 aatggaaggc gtcgccaacg tgcatctcga tgcactggag cacggcgaca ccattgcctt
    27541 tatgcacagc gtgcaggatg gcgcagcgag caaaagctac ggcctggcgg ttgcagctct
    27601 ggccggcgtg ccaaaagagg ttattaagcg cgcacggcaa aaactgcgtg agctggaaag
    27661 catttcgccg aacgccgccg ctacgcaagt ggatggtacg caaatgtctt tgctgtcagt
    27721 gccagaagaa acgtcgcctg cggtcgaagc actggaaaat ctcgacccgg attcactgac
    27781 tccgcgtcag gcgctggagt ggatttatcg cttgaagagt ctggtgtaat aacaattccc
    27841 gatagtcttt tgctatcggg aatattaacg acaactgacg aatcaaataa aaataccctg
    27901 tataatagga aatcttattt tacagggtaa aaccatgcca tctacacgct accaaaaaat
    27961 caatgcccat cactatcacc atatatgggc cgttggtgat attcatggtg actatcaatt
    28021 attacaatcc cgcttacatc aactctcttt ttgtcctgaa accgacttgc ttatttctgt
    28081 cggcgataac atcgatcgtg ggcccgagag tcttaacgtg ctgcgcctgt taaaccagcc
    28141 ctggtttatc tccgtcaaag gcaaccacga agcgatggcg cttgaggcat tcgaaactgg
    28201 cgatggaaat atgtggcttg ccagcggtgg tgactggttt ttcgatttaa atgattcaga
    28261 gcaacaagag gcaatagatc tgttgctgaa attccatcac cttccacata ttattgaaat
    28321 cactaacgac accataaaat atgtcatcgc acatgcagat tatccgggga aggagtatca
    28381 gtttggcaaa gaaatagcgg agagcgaatt actctggcct gttgatcgtg tgcagaaatc
    28441 acttaatagc ga
//
LOCUS       DAZUCC010000041        28070 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZUCC010000041 DAZUCC010000000
VERSION     DAZUCC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.041"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..88
                     /locus_tag="ACHY09_001588"
     tRNA            12..88
                     /locus_tag="ACHY09_001588"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Met,seq:cat)
     gene            137..211
                     /locus_tag="ACHY09_001589"
     tRNA            137..211
                     /locus_tag="ACHY09_001589"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:169..171,aa:Gln,seq:ctg)
     gene            complement(365..1540)
                     /gene="ubiF"
                     /locus_tag="ACHY09_001590"
     CDS             complement(365..1540)
                     /gene="ubiF"
                     /locus_tag="ACHY09_001590"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFS3884575.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            1686..3110
                     /gene="miaB"
                     /locus_tag="ACHY09_001591"
     CDS             1686..3110
                     /gene="miaB"
                     /locus_tag="ACHY09_001591"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFS3884576.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3263..4303
                     /gene="ybeZ"
                     /locus_tag="ACHY09_001592"
     CDS             3263..4303
                     /gene="ybeZ"
                     /locus_tag="ACHY09_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS3884577.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4300..4767
                     /gene="ybeY"
                     /locus_tag="ACHY09_001593"
     CDS             4300..4767
                     /gene="ybeY"
                     /locus_tag="ACHY09_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS3884578.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            4857..5735
                     /gene="corC"
                     /locus_tag="ACHY09_001594"
     CDS             4857..5735
                     /gene="corC"
                     /locus_tag="ACHY09_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFS3884579.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5760..7298
                     /gene="lnt"
                     /locus_tag="ACHY09_001595"
     CDS             5760..7298
                     /gene="lnt"
                     /locus_tag="ACHY09_001595"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFS3884580.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKH"
     gene            complement(7376..9253)
                     /locus_tag="ACHY09_001596"
     CDS             complement(7376..9253)
                     /locus_tag="ACHY09_001596"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS3884581.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLLSSVFLHSSVSHLLMNMFALLVVGGAVERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLSSGAGTHHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWFVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANAVSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVTDIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLIPGMVRGDIVRINTITHQKEDFPTGDARGIVSAMGFRPKNGEIIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            9708..10616
                     /gene="gltI"
                     /locus_tag="ACHY09_001597"
     CDS             9708..10616
                     /gene="gltI"
                     /locus_tag="ACHY09_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFS3884582.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10786..11526
                     /gene="gltJ"
                     /locus_tag="ACHY09_001598"
     CDS             10786..11526
                     /gene="gltJ"
                     /locus_tag="ACHY09_001598"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFS3884583.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            11526..12200
                     /gene="gltK"
                     /locus_tag="ACHY09_001599"
     CDS             11526..12200
                     /gene="gltK"
                     /locus_tag="ACHY09_001599"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFS3884584.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12200..12925
                     /gene="gltL"
                     /locus_tag="ACHY09_001600"
     CDS             12200..12925
                     /gene="gltL"
                     /locus_tag="ACHY09_001600"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFS3884585.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            13043..13978
                     /gene="rihA"
                     /locus_tag="ACHY09_001601"
     CDS             13043..13978
                     /gene="rihA"
                     /locus_tag="ACHY09_001601"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFS3884586.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPITIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(14023..14505)
                     /gene="ybeL"
                     /locus_tag="ACHY09_001602"
     CDS             complement(14023..14505)
                     /gene="ybeL"
                     /locus_tag="ACHY09_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFS3884587.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            14740..17322
                     /gene="leuS"
                     /locus_tag="ACHY09_001603"
     CDS             14740..17322
                     /gene="leuS"
                     /locus_tag="ACHY09_001603"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS3884588.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            17337..17918
                     /gene="lptE"
                     /locus_tag="ACHY09_001604"
     CDS             17337..17918
                     /gene="lptE"
                     /locus_tag="ACHY09_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFS3884589.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            17918..18949
                     /gene="holA"
                     /locus_tag="ACHY09_001605"
     CDS             17918..18949
                     /gene="holA"
                     /locus_tag="ACHY09_001605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS3884590.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHMPLRALFDKH
                     RVWQNRRGMMGEALNRLSHPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            18951..19592
                     /gene="nadD"
                     /locus_tag="ACHY09_001606"
     CDS             18951..19592
                     /gene="nadD"
                     /locus_tag="ACHY09_001606"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS3884591.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            19616..20227
                     /gene="cobC"
                     /locus_tag="ACHY09_001607"
     CDS             19616..20227
                     /gene="cobC"
                     /locus_tag="ACHY09_001607"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFS3884592.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            20487..20804
                     /gene="rsfS"
                     /locus_tag="ACHY09_001608"
     CDS             20487..20804
                     /gene="rsfS"
                     /locus_tag="ACHY09_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS3884593.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            20808..21275
                     /gene="rlmH"
                     /locus_tag="ACHY09_001609"
     CDS             20808..21275
                     /gene="rlmH"
                     /locus_tag="ACHY09_001609"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS3884594.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            21306..23207
                     /gene="mrdA"
                     /locus_tag="ACHY09_001610"
     CDS             21306..23207
                     /gene="mrdA"
                     /locus_tag="ACHY09_001610"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFS3884595.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            23210..24322
                     /gene="mrdB"
                     /locus_tag="ACHY09_001611"
                     /gene_synonym="rodA"
     CDS             23210..24322
                     /gene="mrdB"
                     /locus_tag="ACHY09_001611"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFS3884596.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            24333..25421
                     /gene="rlpA"
                     /locus_tag="ACHY09_001612"
     CDS             24333..25421
                     /gene="rlpA"
                     /locus_tag="ACHY09_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFS3884597.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGLYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            25561..26772
                     /gene="dacA"
                     /locus_tag="ACHY09_001613"
     CDS             25561..26772
                     /gene="dacA"
                     /locus_tag="ACHY09_001613"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFS3884598.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            26882..27145
                     /gene="ybeD"
                     /locus_tag="ACHY09_001614"
     CDS             26882..27145
                     /gene="ybeD"
                     /locus_tag="ACHY09_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFS3884599.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            27246..27887
                     /gene="lipB"
                     /locus_tag="ACHY09_001615"
     CDS             27246..27887
                     /gene="lipB"
                     /locus_tag="ACHY09_001615"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFS3884600.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         6852 a         7321 c         7225 g         6672 t
ORIGIN      
        1 agaaacaatc tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
       61 acaggttcga atcccgtcgt agccaccaaa ttctgaatgt gtcgaatatg ttcggcaaat
      121 tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc accggattct gattccggca
      181 ttccgaggtt cgaatcctcg taccccagcc acattaaaaa agctcgcctc ggcgagcttt
      241 ttgcttttct gcgtatattc aatgccgaat gcgatgttga ctcgtcttat ccttcaaagt
      301 cggatgcgac gctgccgcgt cttatccgac ctacggttgg catgcatcca gcaatgttgt
      361 gaggctacaa ccctaacgca tatttcagcg ccttacgttt caacacgcca gcacgctccg
      421 ccgccatcaa accaagatta cgcacaaaac gcagcggtgg cagattattg ctgaatccgg
      481 cataaaacag atccatacca ctttgcatca taaagttatc cgccatacgc cgcatctggt
      541 aacgcttgag cacaggataa ctggcccacg cttcgccgta gctgcgggcg ttcaccagaa
      601 cgtcaatcag ggcatcgaca tcacgataac caagattcac cccctgcccc gccagcggat
      661 ggatggtatg cgcggcatcg cccaccagag caagccctgg ttgcacatac tgcaacgcat
      721 ggcgacgtat cagcggaaac gcaccagcgg caagcggtgt cacgtaaccc agacgcgacg
      781 ggaaatgctt cgcgatttcc gcctgtagct gcgccatatt catattctgc aactggcgaa
      841 tgcgcgccgg agagtcatac cacaccagcg atgcccagtt atcaaacaac ggcagaaacg
      901 cacgcggtcc gtccggggta aattgctgcc aggtgctgtc gccgggatcg ttctcacact
      961 gtacgctaat caacatacac gactgcgcat actgccaggc atgaacgcca attcccgcca
     1021 tctgtcgcac ctgcgaattt gcgccgtcgg caccaatcac cagcttcgcg aggatcgttt
     1081 caccgccctt cagttccagc tcctgaagat cattatggcg atgcagtgca atcagcgagg
     1141 ttggcacacg taacgttact ttcggatgcg cttccagcgc ctgccacaac gcctgttgca
     1201 ggacagtgtt ttccaccatg tagccaagca acggtagctt aagttcagcg gcgtcaaaca
     1261 ccacatgcgc cgtttcccac tcccacgttt ccagtctgcg gtaaggatgg caacgcatag
     1321 cctgtactgc atcccagacc cctaaccctt taagcaatga taccgaagcc gcactaatcg
     1381 ccgagatccg cacgtccggc tggctgtcag cgacaaacgg tgcaggttgg gcatgttcga
     1441 taaccattac cgaaaatccg tgctgagcca gtcccagcgc cagtgcgccg ccgaccattc
     1501 ctccgccgac aatggcaatt tccgttggtt gatttgtcat ggtcaatcat cctgttagca
     1561 atatgcttta agtttacagg atttttaccc ccaagggctg ataacgctca tactggtcac
     1621 aacggcagca aagcattaca ctatgcgccc tgcattcctg gctactattt cgcaagagca
     1681 agtcgatgac caaaaaactc catattaaaa cctggggctg tcagatgaac gagtacgatt
     1741 catcgaagat ggccgatctg ctggatgcca cccacggcta tcaactgacc gacgtggcgg
     1801 aagaagcgga tgtgctgctg ctgaacacct gctcaatccg cgagaaggct caggaaaaag
     1861 tcttccatca gttgggtcgc tggaaactgt taaaagagaa gaatccagac ctgattatcg
     1921 gcgtcggcgg ctgcgtggca tcgcaagaag gcgagcacat tcgccagcgc gcccactatg
     1981 tcgatattat ttttgggccg caaacgctgc accgtctgcc ggagatgatc aactccgtgc
     2041 gcggcgaccg cagcccggtt gtagatatca gcttcccgga aatcgagaag tttgaccgtc
     2101 tgccggaacc gcgcgccgaa gggccgaccg cgtttgtctc catcatggaa ggctgcaata
     2161 aatattgcac ctactgcgta gtgccttaca cccgtggtga agaggtaagc cgtccgtccg
     2221 acgatattct gtttgagatt gcccagcttg ctgctcaggg cgtgcgtgaa gttaacctgc
     2281 tcggccagaa cgtgaacgcc tggcgcggtg agaactacga cggcaccacc ggatcgtttg
     2341 ccgatctgct gcgtctggtt gcggcgatcg acgggatcga tcgtattcgc tttaccacca
     2401 gccatccgat cgaattcacc gacgatatca tcgaagtgta ccgcgacacg ccggagctgg
     2461 tgagcttcct gcatctgccg gtacagagcg gttccgatcg cattctgaac ctgatgggac
     2521 gtacccatac agcgctggag tacaaagcga tcatccgtaa gctgcgtgcg gcgcgtccgg
     2581 atattcagat cagttctgac ttcatcgttg gcttccctgg cgaaaccacc gaagacttcg
     2641 agaaaacgat gaagctgatt gccgacgtca atttcgacat gagctacagc tttatcttct
     2701 ccgctcgtcc aggtacacca gccgccgata tggttgatga tgttccggaa gaagagaaga
     2761 agcagcgcct gtatattcta caagaacgta tcaatcagca agcaatggca tggagccgcc
     2821 ggatgctcgg taccacccag cgtattctgg tagaaggtac atcgcgtaag agcatcatgg
     2881 agctttccgg ccgtactgaa aataaccgcg tcgtcaactt cgaaggtacg ccggatatga
     2941 tcggtaaatt cgtcgatgta gaaattaccg acgtctaccc gaactctctg cgcggtaaag
     3001 tggtgcgaac tgaagatgaa atgggtctgc gcgtggcaga aacaccggaa tcggtgattg
     3061 cccgtacccg caaagaaaac gaccttggcg tgggttatta tcagccgtaa ttctcaggcc
     3121 cgccgttccg gtgggccttg ttttttctct ctttatcccc atctttatcg caatgcttgc
     3181 gcctttttcc tgtggcgtga ataatttcct tataggccgg tgaatgttca gcttccggcg
     3241 gcatacagat aagaggaacg gtttgaacat agacactcgc gaaatcaccc tggagccagc
     3301 agacaatgcg cgtctgttga gcctgtgcgg cccgtttgat gacaacatca aacaactcga
     3361 acgccgtctc ggcatcgaaa tcaatcgccg cgataaccac tttaaactga ccggccgtcc
     3421 gatttgcgtc accgctgcgg cagacattct gcgtagcctg tatgtcgata ctgccccgat
     3481 gcgcggtcag attcaggata tcgaaccgga acagatccac cttgcgatta aagaagcgcg
     3541 ggtactggag caaagcgcgg agagcgtgcc ggagtacggc aaagcggtca atatcaaaac
     3601 caaacgcggc gtaattaagc cgcgcacgcc aaaccaggcg cagtacatcg ccaatattct
     3661 cgaccatgac attaccttcg gcgttggccc ggcgggtacg ggtaaaacct acctggcagt
     3721 ggctgcggca gttgatgccc tggagcgtca ggaaattcgc cgtattctgc tgactcgtcc
     3781 ggcagtagaa gccggtgaga aactgggctt cctgcctggc gatttaagcc agaaagtaga
     3841 cccgtatctg cgcccgctgt acgacgcgct gtttgaaatg ctgggctttg agaaagtcga
     3901 gaaactgatt gagcgcaacg ttattgaagt cgcaccgctg gcctatatgc gtggtcgtac
     3961 gctgaacgac gcgtttatca ttcttgatga gagccagaac accaccatcg aacagatgaa
     4021 gatgttcctg acccgtatcg gttttaactc aaaagcggtt atcaccggcg acgtcacaca
     4081 gatcgacctg ccgcgtaata ccaagtctgg tttgcgtcat gccatcgaag tactggctga
     4141 tgtcgaagag atcagcttta acttcttcca cagcgaagac gtggttcgtc acccggtggt
     4201 ggcgcgtatc gttaacgcct atgaagcctg ggaagaagcc gaacaaaaac gtaaagcggc
     4261 gctggcagca gaacgcaagc gcgaagaaca ggaacaaaaa tgagtcaggt gatcctcgat
     4321 ttacaactgg catgtgaaga taattccggg ttaccggaag agagccagtt tcagacatgg
     4381 ctgaatgcgg tgatcccgca gtttcaggaa gaatcggaag tgactattcg cgtggtcgat
     4441 accgccgaaa gccacagtct gaatctgacc tatcgcggta aggataagcc gaccaacgtg
     4501 ctctccttcc cgtttgaagt accgcctggc atggaaatgt cgctactggg cgatctggtt
     4561 atctgccgtc aggtggttga gaaggaagct caggagcaag gcaaaccact ggaggcgcac
     4621 tgggcgcata tggtggtgca cggcagtctg catttgttag gttacgatca catcgaagat
     4681 gacgaagcag aagaaatgga agccatcgaa acagagatta tgcttgctct gggctatgag
     4741 gatccgtaca ttgccgagaa agaataattt gccaggcatt tgactggcag acgtccccgc
     4801 cgttggtgat taacgacggc gttttgatta actaacatga gaacccatac gacgccatga
     4861 gcgacgacaa ttcacacagt agtgacacga taagcaacaa gaagggattt ttctccctgt
     4921 tactcagcca acttttccac ggtgaaccga aaaaccgtga cgaactgctg gcgctgatcc
     4981 gtgattccgg gcagaacgac cttatcgacg aagatacgcg cgatatgctc gaaggggtga
     5041 tggacatcgc agaccaacgc gttcgcgaca tcatgatccc ccgctcccag atgattaccc
     5101 tgaaacgcaa ccagacgctg gacgaatgcc ttgatgtcat catcgagtcc gcccactcac
     5161 gtttcccggt gattagcgaa gacaaagatc acattgaagg gattctgatg gcgaaagact
     5221 tgctgccgtt tatgcgcagc gatgctgaag ccttcagcat ggacaaagtg ttacgtcagg
     5281 cggttgtcgt tcctgaaagt aagcgcgtag accggatgct gaaagagttt cgctctcagc
     5341 gttaccacat ggcgatcgtc attgacgaat tcggtggggt ttccggtctg gtgaccattg
     5401 aagacatcct ggaactgatt gttggtgaga ttgaagacga gtatgacgaa gaagatgata
     5461 tcgacttccg tcagctgagt cgtcatacct ggaccgtgcg cgcactggct tctattgaag
     5521 acttcaacga agcgttcggc acccacttta gcgatgaaga ggttgacact atcggtggtc
     5581 tggtgatgca ggcatttggg catcttccgg cgcgtggcga aaccatcgac atcgacggtt
     5641 accagttcaa agtggcgatg gccgacagtc ggcgtattat tcaggttcat gtcaaaatcc
     5701 cggatgactc accccagccg aagctggatg aataaaaccg aaactggata gataactaca
     5761 tggcttttgc ctcattaatt gaacgccagc gcattcgcct gctgctggcg ttattattcg
     5821 gtgcctgcgg aacgctggcc ttctctcctt acgacgtctg gcctgcggcg attatttcgc
     5881 tgatggggct tcaggcgttg acctttaacc gccgtccact ccagtctgcc gctattggct
     5941 tttgctgggg atttggcctc tttggcagcg gtattaactg ggtctatgtc agcatcgcga
     6001 cctttggcgg aatgcctggc ccggttaaca tcttcctggt ggtactgctg gcggcgtatt
     6061 tgtcgctgta taccggactg tttgctggcg tgctgtcgcg tctgtggccg aaaaccacct
     6121 ggctgcgcgt ggcgattgcc gcccctgctc tctggcaagt gaccgagttt ctgcgcggtt
     6181 gggtactaac aggcttcccg tggttacagt tcggctatag ccagattgat ggcccgttaa
     6241 aagggctggc accgataatg ggcgtggaag ccattaactt cctgctgatg atggttagcg
     6301 gcctgctggc actggcgttg gtcaaacgca actggcgtcc gctggtggtg gccgtcgtgc
     6361 tgtttgccct tcccttcccg ctgcgttaca tccagtggtt taccccgcaa ccggaaaaaa
     6421 ctattcaggt ttcgatggtt cagggcgata ttccgcaatc gctgaaatgg gacggagacc
     6481 agctacttaa tacgctgaag atatactaca acgcaacggc accgctgatg ggcaaatcat
     6541 cgttgattat ctggccggag tcggcgataa ccgatctgga aattaatcag caaccgttcc
     6601 tcaaagctct ggacggtgag ttgcgcgata aagggagctc gctggtcacc gggattgtcg
     6661 acgcgcgtct caataaacag aaccgctacg atacctacaa caccatcatc acactgggta
     6721 aaggcgcacc gtacagctat gaatcagccg atcgctataa caaaaaccat ctggtgccgt
     6781 ttggcgagtt tgtcccactg gagtcgatac tgcgtccgtt agcaccgttc tttgatttgc
     6841 cgatgtcttc attcagccgc ggaccatata ttcagccacc gctgtcgttg aatggcattc
     6901 aacttactgc ggccatttgc tacgaaatca ttctcggcga gcaagtgcgc gataacttcc
     6961 gcccggatac cgactatctg ctgactatct ccaacgatgc gtggtttggt aagtctattg
     7021 gtccatggca gcacttccag atggcgcgaa tgcgtgcgct ggagctggcg cgcccactgt
     7081 tgcgcagcac caacaacggc attacggcgg tgattggccc gcagggtgag attcaggcga
     7141 tgatcccgca gttcacccgc gaggtgttaa ccactaacgt gacgccgact accggactca
     7201 caccgtacgc acgtaccggc aactggccgc tgtgggtgct gacggcactg tttggttttg
     7261 cagctgtgct gatgagtctg cgagcacgta aacattaatg tgcctgatgt atccggcatg
     7321 aacaaaacat actttctcat tgagttgtgg ggcatgatta tcatgccctt ttttcttacg
     7381 gttcagggat acaagccaca tcgccatatt ggggatgccg aataattgcc tgcggatcaa
     7441 gcgagcgcgt cctgtccatt tggattaacc tgttcttcgc cataacaaat atctttctac
     7501 catccgctga aaaacgaagg tgattaacgt agctggcagg ataaacgcca ataatctgcc
     7561 cggcattaat attgagcagg atgacataac cttgcaggcc atatgacacc aacgctaaat
     7621 attgaccgtc cgggctaata ccctcaacgg aataaacgct cctgctacac cattgcgtca
     7681 taatgctggc ttgttgatcc cctacgctta tacgtgaaat cccctggcta tcggcaaaaa
     7741 taatctcgcc attttttggt cggaatccca tcgcagaaac gataccacgc gcatcacctg
     7801 tgggaaagtc ttctttctga tgcgtgatgg tattgatgcg tacaatgtcg cctctgacca
     7861 taccgggtat caacaatgtt ttttcatccg gggaaagtgc cataacccag gcgcctggtt
     7921 taccgctcgt ccccgtcccg tcgtatttct ccagcgggat atccgtcaca tgctgataag
     7981 tcaccagatc aatggctgac acactattac tggtgcgatt catcacccag gcttttgtgc
     8041 catcacgcga gaggataagg ctgtcaggat gggcgccggt ggtaatcgac tgaataatct
     8101 cccccgtcgc cacgttaatg accgatactg cattcgcttc aaatgaagtc gcataaatca
     8161 acttttcatc caggcttaat gccagcgatc tgacgccctg atctttacag ccaccacaat
     8221 gtttgactga cttttctttc gctatgggcg ctgtaaaggt atgcaggatt ttcttttgtc
     8281 cgaggtcgaa aacaacaatg ctgttggtgt tttcaattgc ggcatacaac gtattaccat
     8341 cccgactgat cgccatatca tggatatcgg caatctcagc gagtaaatca ccactggcct
     8401 gctcacgact taccggagcg gataatgttt ccctgagggc gttgcgctcc tccactaact
     8461 gctgcttttt ttgtcgtcgc tcttgttcaa tctcctgcgg ataaaacgct tcacgcaggc
     8521 tttgcctgac ctgtaaaact gattcatcaa tctgctgctg cacaaaccag atagtcccgg
     8581 tcagaagaag agtgaccgca acaataatcc cgccttccgt aacgagccga ttttgcccaa
     8641 ctaaacgcgc actcagccaa cccaacgcgc cccccgcaat caggccgcca atgtggcaag
     8701 cgttatctat tcctgcttgc cgggcaccat acaacagtgt cagcgccacc atacccaaca
     8761 gagaaaatac tcgccgctgg tttttatggt gtgtacccgc acctgaagaa agctgtgttg
     8821 ctatcgcagc gcccgctatt cccataattg ccccggatgc cccaacgctg atgactatct
     8881 gctcactttc gcgtaacgca taacaggcgc tgatcagccc accaaagatg ccggagaata
     8941 accagataat caggagtcgc catttcccca gaatacgttc caccgctccc cccaccacca
     9001 ggagtgcaaa catattcatc agcaaatgag aaacactgct gtgcagaaag acactgctca
     9061 ataagcgcca ggattcaccc gacaacgtga gagaagcaac gttagcaccg agatggataa
     9121 gtgcggattg atcgacttct tcataactca tccattgtgc gaggtaacaa aatatcccaa
     9181 cacagagcgc aaaaaggata agggttattg cgggtaattg aacatttaac ggcttaaccg
     9241 aagatgcaga cataaggtga ttcttccttg aaaagacagg taaaaagcat ttttcccgtg
     9301 tgcagttatc atcccggcaa acgtcccatg ccgaaaagat aaccctgtcc gaaatatgac
     9361 tatttcagcc taacattaca atcgtaatta attcacctat aagtattttt tggcatgtaa
     9421 gttgctttat ttactgtgta agcaatgaaa tcagccgcga cgcaaccggg ttgcaccacc
     9481 acaaatcagc ggtagcacca aaaaggtgcg atttaagaat tttgcttcgg aatggtgcgg
     9541 cgcatctcgc aaaaacaaac aataaaacag caatatcttt acatttagcc aaactaaatg
     9601 ttacctaaca agcacaacac tgcacaataa agttgcagat gataacaaca caaacactca
     9661 caacgggtat ccatgcgttc ttaacgcaga agataaagga gttggatatg caattacgta
     9721 aacctgccac agcaatcctg gccctggcgc tttccgcagg actggcacag gcagatgacg
     9781 ccgccccggc agcaggcagt acgctggaca aaatcgccaa aaacggcgtg attgtcgtcg
     9841 gtcaccgtga atcttcagta cctttctctt attacgacaa tcagcaaaaa gtggtgggct
     9901 actcgcagga ttactccaac gccattgttg aagcagtgaa aaagaaactc aacaaaccag
     9961 acttacaggt gaaattgatt ccgatcacct cacaaaaccg tattccactg ctgcaaaacg
    10021 gcactttcga ttttgaatgc ggttctacca ccaacaatgc cgaacgccaa aaacaggcgg
    10081 ctttctctga cactattttc gtggtcggta cgcgcctgct gaccaaaaag ggcggcgata
    10141 tcaaagattt tgccgacctg aaaggcaaag ccgtagtcgt cacttccggc accacctctg
    10201 aagttttgct caacaaactg aatgaagagc aaaaaatgaa tatgcgcatc atcagcgcca
    10261 aagatcacgg tgactctttc cgtaccctgg aaagcggtcg tgccgttgcc tttatgatgg
    10321 atgatgctct gctggccggt gaacgtgcga aagcgaagaa accagacaac tgggaaatcg
    10381 tcggcaagcc gcagtctcag gaagcctatg gttgcatgtt gcgtaaagac gatccgcagt
    10441 tcaaaaagct gatggatgat accatcgctc aggtgcagac ctccggtgaa gcggaaaaat
    10501 ggtttgataa gtggttcaaa aatccaattc cgccgaaaaa cctgaacatg aatttcgaac
    10561 tgtcagacga aatgaaagca ctgttcaaag aaccgaatga caaggcactg aactaattac
    10621 aagaaccagg ggcggaaatt ccagccctct cgattgttac gtagcacgga cagactatac
    10681 gcctggtggt cgttccccat cgggcctgaa aaccgcaata cgctgggtaa caatcttcga
    10741 gggtagcagt taacgctgct accctctttt ttctggagta gatttatgtc tatagactgg
    10801 aactggggta tttttttaca acaagccccg ttcggcaaca ccacctatct tggttggatc
    10861 tggagtggtt ttcaggtgac gatcgctttg tcgatctgcg cctggattat cgctttcctc
    10921 gtcggttcat ttttcggcat tttacgtacc gttccaaacc gttttctctc cggtctgggt
    10981 acgttgtatg tcgaactgtt ccgcaacgtg ccgttgattg tgcaattctt tacctggtac
    11041 ctggtgatcc cggagctgct gccagagaaa atcggcatgt ggtttaaggc agagctggat
    11101 cccaatattc agtttttcct ttcatccatg ctctgcctgg ggctgtttac cgccgcccgt
    11161 gtttgcgaac aggtacgcgc cgctattcag tcgctgccgc gtgggcaaaa aaatgccgcg
    11221 ctggcgatgg gtctgacgct accgcaggct taccgctacg tactgctgcc taatgcttat
    11281 cgcgttatcg tcccgccgat gacctcagag atgatgaacc tggtgaaaaa ctccgccatc
    11341 gcctcgacta tcggtctggt ggatatggcg gcgcaggcgg gtaaattgct cgattactcg
    11401 gcccacgcct gggagtcgtt taccgccatc acgctggcgt atgtattgat taacgctttt
    11461 atcatgctgg tgatgacgct ggttgaacgt aaagttcgtc tgcccggcaa catggggggc
    11521 aaataatgta cgagtttgac tggagttcca ttgttccttc cctgccatat ctgctcgacg
    11581 ggctggtgat cacgctgaaa atcaccgtca cggcagtggt gattggtatt ttgtggggca
    11641 ccatgctggc ggtgatgcgc ttgtccagct ttgcgcctgt tgcgtggttt gccaaagcct
    11701 atgttaacgt attccgctcg attcctttag tcatggtttt gctgtggttt tacctgattg
    11761 tgccgggatt tctgcaaaac gtgctgggat tatcgccaaa aaatgatatc cgtttaattt
    11821 cggcaatggt ggcgttttcg atgtttgaag cggcctacta ttcagagatt atccgtgccg
    11881 gtattcaaag catttcccgt ggtcagtcga gcgccgcgct ggcgttgggg atgactcact
    11941 ggcagtcgat gaaactgatt attctgccgc aggctttccg cgcgatggtg ccgctgctgc
    12001 tcactcaggg catcgtactg ttccaggata cctcgctggt gtatgtgtta tctctggccg
    12061 atttcttccg taccgcttca accattggtg agcgtgatgg tacgcaggtt gagatgatcc
    12121 tgtttgccgg atttgtttat ttcgttatta gccttagcgc gtcgttgttg gtcagctact
    12181 tgaaaagaag gacagcataa tgattaccct gaaaaatgtt tcaaaatggt atggtcactt
    12241 tcaggtgctg accgactgct caaccgaagt gaaaaaaggc gaagtggtgg tggtttgcgg
    12301 cccgtctggt tccggcaaat caacgctgat taaaaccgtc aacggcctcg aaccggtgca
    12361 gcaaggtgaa atcaccgtcg atggcatcgt ggttaacgac aagaaaaccg atctggcaaa
    12421 gctgcgttcc cgcgtcggga tggtattcca gcatttcgag ctgttccctc atctgtcgat
    12481 tatcgaaaac ctgaccctgg cgcaggtgaa agtgcttaaa cgcgataaag cgccggctcg
    12541 tgaaaaagca ttgaaattgc tggagcgcgt cgggctttcc gcacatgcca ataagtttcc
    12601 ggcgcagctt tccggcggtc agcagcagcg tgtggcgatc gctcgcgcat tgtgtatgga
    12661 tcctattgcc atgctgttcg acgaaccgac atcggcgctg gatccggaga tgatcaacga
    12721 agtgctggac gtgatggtgg aactggcgaa cgaaggaatg accatgatgg tggtgaccca
    12781 cgaaatgggc tttgcccgta aagtggcgaa tcgggtgatc tttatggacg agggtaaaat
    12841 tgtcgaagac tcgccgaaag acgctttctt cgatgatccg aaatcggacc gcgcaaaaga
    12901 cttcctcgcg aaaatcctgc attaatcact ctggcgcgca tacccaagtg cgcgccatct
    12961 cacgctttat cgtttcgctc ctcgtatctg ttacatccta gcggtaccct tgtcgcaaag
    13021 aagcacacaa caaggagcaa caatggcact gccaattctg ttagattgcg acccaggtca
    13081 tgacgacgct atcgcaatag ttcttgccct cgcctcacca gagcttgatg tcaaagcaat
    13141 tacgtcttcc gccggaaacc agacaccaga aaaaacctta cgcaatgttc tgcgtatgct
    13201 gaccttgctt aatcgcaccg atattccggt agcaggcggc gcggtaaaac cgttaatgcg
    13261 taatttgatt attgcggaca atgtgcacgg cgaaagcggt ctcgacggcc cggcattacc
    13321 ggaaccgacg ttcgcaccgc aaaactgtac ggcggtggag ctaatggcga aaacgctgtg
    13381 tgaaagtgag gaacctatca ccattgtttc aaccggaccg caaactaacg ttgccttgct
    13441 gctcaatagc cacccggaac tgcatagcaa aattgcccgt atcgtgatta tgggcggcgc
    13501 aatggggctt ggtaactgga cgcctgcggc tgaatttaat atttacgttg acccggaagc
    13561 ggcagaaatt gtcttccagt caggaattcc ggtggtgatg gccggtctgg atgttactca
    13621 caaagcacaa atccacgttg aagacactga gcgtttccgc gcgattggca accctgtttc
    13681 aaccattgtt gccgaactgc tggatttctt cctcgaatat cataaagacg aaaaatgggg
    13741 ctttgtcggc gcaccactgc atgacccatg caccatcgcc tggctgttga aaccggagtt
    13801 gtttaccact gttgagcgct gggttggcgt ggaaacacaa gggaaatata cccagggtat
    13861 gacggttgtt gattattatt atctgactgg caataaaccg aatgccaccg taatggtcga
    13921 tgttgatcgt cagggctttg ttgatttact ggctgatcgt ctgaaatttt acgcttaatc
    13981 actcgccgga tgacatcacg ccatccggca ccactcattt cattacggct cgaacggacg
    14041 tctctggaac tgatcatgac cacatttcgg gcatagcgtc agcacttccg gtgtatagat
    14101 cgggagatgg aagtgacatt tctcgcagac cagatttccc agccctacca cttctccgct
    14161 gtgataaacc ccgtgatgat tgaggtcctg gaaaacttcg cgccattcaa gctgcgtttt
    14221 atcggtgatg tctgccagct cctgccacaa gctttcttta atcacccgca taaagacgct
    14281 gtcagattct tctttcaggc tctcttcata gctcatggcg aactcttcca ggtcacgtct
    14341 gacagctcgc gtcagctcat cgacctcggt tcgcgttaac tcccctgttt ttattacgcg
    14401 ctcgcgcgcc tgttccacca gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgct
    14461 cagtgacgcg accagttcac ggtaatattg agcaaccttg ttcatcgttt catctcccgg
    14521 gtcgttaact gactataata atagacgttc tttgttcctt gctttgctaa tacgcccaca
    14581 aactctgtaa attaatgtat gaataatccg ggcatcattt caacgtcgag tgcgcgaaag
    14641 gctgttttga cgcggacgtt tgggctatgc tatgcggatc tgaaaaacca cctcaacgct
    14701 acatttgtag ccgtattgaa aacaggacca ctggctgcca tgcaagagca ataccgcccg
    14761 gaagagatag aatccaaagt acagcttcat tgggatgaga agcgcacatt tgaagtaacc
    14821 gaagacgaga gcaaagagaa gtattactgc ctgtctatgc ttccctatcc ttctggtcga
    14881 ctacacatgg gccacgtacg taactacacc atcggtgacg tgatcgcccg ctaccagcgt
    14941 atgctgggca aaaacgtcct gcagccgatc ggctgggacg cgtttggtct gcctgcggaa
    15001 ggcgcggcgg tgaaaaacaa caccgcaccg gcaccgtgga cgtacgacaa catcgcgtac
    15061 atgaaaaacc agctcaaaat gctgggcttt ggttatgact ggagccgcga gctggcaacc
    15121 tgtacgccgg aatactaccg ttgggaacag aaattcttca ccgagctgta taaaaaaggc
    15181 ctggtatata agaagacttc tgcagtcaac tggtgcccga acgaccagac cgtactggcg
    15241 aacgaacaag ttatcgacgg ctgctgctgg cgctgcgata ccaaagttga gcgtaaagag
    15301 atcccgcaat ggtttatcaa aatcaccgct tacgctgacg agctgctcaa cgatctggat
    15361 aaactggatc actggccaga caccgttaaa accatgcagc gtaactggat cggtcgttcc
    15421 gaaggcgtgg aaatcacctt caacgttaac gactatgaca acacgctgac cgtttacact
    15481 acccgcccgg acacctttat gggttgtacc tacctggcgg tggctgcggg tcatccgctg
    15541 gcacagaaag cggcggaaaa taatcctgaa ctggccgcct ttattgacga atgccgtaac
    15601 accaaagttg ccgaagctga aatggcgacg atggagaaaa aaggcgtcga tactggcttt
    15661 aaagcggttc acccattaac gggcgaagaa attcccgttt gggcagcaaa cttcgtattg
    15721 atggaatacg gcacgggcgc agttatggcg gttccgggtc acgaccagcg cgactacgag
    15781 tttgcctcta aatacggcct gaacatcaaa ccggttatcc tggcagctga cggctctgag
    15841 ccagatcttt ctcagcaagc cctgactgaa aaaggcgtgc tgttcaactc tggcgagttc
    15901 aacggtcttg accatgaagc ggccttcaac gccatcgccg ataaactgac tgcgatgggc
    15961 gttggcgagc gtaaagtgaa ctaccgcctg cgcgactggg gtgtttctcg tcagcgttac
    16021 tggggcgcgc cgattccgat ggtgacgctg gaagacggta ccgtaatgcc gaccccggac
    16081 gaccagctgc cggtgatcct gccggaagat gtggtgatgg acggcattac cagcccgatt
    16141 aaagcagatc cggagtgggc aaaaactacc gttaacggta tgccagcgct gcgtgaaacc
    16201 gacactttcg acacctttat ggagtcctcc tggtactatg cgcgctacac ttgcccggag
    16261 tacaaagaag gtatgctgga ttccaaagcg gctaactact ggctgccggt ggatatctac
    16321 attggtggta tcgaacacgc cattatgcac ctgctctact tccgcttctt ccacaaactg
    16381 atgcgtgatg caggcatggt gaactctgac gaaccagcaa aacagttgct gtgtcagggt
    16441 atggtgctgg cagatgcctt ctactatgtt ggcgaaaatg gcgaacgtaa ctgggtttcc
    16501 ccggttgatg ctatcgttga acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg
    16561 gcaggccatg aactggttta taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt
    16621 atcgacccgc aggtgatggt tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg
    16681 tttgcttctc cggctgatat gactctcgaa tggcaggaat ccggcgtgga aggggctaac
    16741 cgcttcctga aacgtgtctg gaaactggtt tacgagcaca cagcgaaagg tgatgttgcg
    16801 gcactgaacg ttgatgcgct gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa
    16861 acgatcgcta aagtgaccga tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg
    16921 gcgattatgg agctgatgaa caaactggcg aaagcaccga ccgatggcga gcaggaccgc
    16981 gccctgatgc aggaagcact gctggccgtt gtccgtatgc ttaacccgtt caccccgcac
    17041 atctgcttca cgctgtggca ggaactgaaa ggcgaaggcg atatcgacaa cgcgccgtgg
    17101 ccggttgctg acgaaaaagc gatggtggaa gactccacgc tggtcgtggt gcaggttaac
    17161 ggtaaagtcc gtgcaaaaat caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa
    17221 cgtgctggcc aggaacatct ggtagcaaaa tatcttgatg gcgttaccgt gcgtaaagtg
    17281 atttacgtac ccggtaaact cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc
    17341 gatatctggc aacattgttg ttatctctgg cggtgttaat caccgccggg tgtggctggc
    17401 atctgcgtga taccacgcag gttccttcca ctatgaaggt catgatcctg gactcaggcg
    17461 atccgaacgg gccattaagc cgtgcggtgc gtaaccagtt acgtctaaat ggtgtcgagt
    17521 tgcttgataa agaaaccacg cgtaaggacg ttccatccct gcgtttgggt aaagtgagca
    17581 tcgcgaaaga taccgcatcg gtattccgta acggtcaaac agcagagtat cagatgatca
    17641 tgacggtcaa tgcgaccgtg ttgatccccg gccgtgacat ctacccgatt agcgccaaag
    17701 tcttccgttc gttcttcgat aacccgcaaa tggcgttagc gaaagataac gaacaagaca
    17761 tgatcgtaaa agagatgtac gaccgtgctg ccgaacagct gattcgtaag ctgccaagca
    17821 tccgtgctgc ggatattcgt tccgacgaag aacagacgtc gaccacaacg gatactccgg
    17881 caacgcctgc acgcgtctct accacgctgg gtaactgatg attcggttgt acccggaaca
    17941 actccgcgcg cagctcaatg aagggctgcg cgcggcgtat cttttacttg gtaacgatcc
    18001 tctgttattg caggaaagcc aggacgctat tcgtcaggta gctgcggtac aaggattcga
    18061 agaacaccac actttttcca ttgatcccaa cactgactgg aatgcgatct tttcgttatg
    18121 ccaggccatg agtctgtttg ccagtcgaca aacgctattg ctgttgttac cagaaaacgg
    18181 accgaatgcg gcgatcaatg aacaacttct cacactcacc ggacttctgc atgacgacct
    18241 gctgttgatc gtccgcggca ataaattaag caaagcgcaa gaaaatgccg cctggtttac
    18301 tgctcttgcg aatcgcagcg tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc
    18361 ccgctgggtt gctgtgcgcg caaaacagct caacttagag ctggatgacg cggcgaatca
    18421 ggtgctctgc tactgttatg aaggtaacct gctggcgctg gctcaggcac tggagcgttt
    18481 atcgctactc tggccagacg gcaaactgac attacctcgc gttgaacagg cggtgaatga
    18541 tgccgcgcat ttcacccctt ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg
    18601 cgcgttgcat attcttcagc aactgcgtct ggaaggcagc gaaccggtta ttttgttgcg
    18661 cacattacaa cgtgaactgt tgttactggt taacctgaaa cgccagtctg cccatatgcc
    18721 actgcgtgcg ttgtttgata agcatcgggt atggcagaac cgccggggca tgatgggcga
    18781 ggcgttaaat cgcttaagcc atccgcagtt acgtcaggcc gtgcaactcc tgacacgaac
    18841 ggaactcacc ctcaaacaag attacggtca gtcagtgtgg gcagagcttg aagggttatc
    18901 tcttctgttg tgccataaac ccctggcgga cgtatttatc gacggttgat atgaaatctt
    18961 tacaggctct gtttggcggc acctttgatc cggtgcacta tggtcatctt aaacccgtgg
    19021 aaacgctggc gaatttgatt ggtctgacgc gggtcacaat catccctaat aatgttcctc
    19081 cgcatcgtcc ccagccggaa gcaaacagca tgcagcgtaa acacatgctt gaactggcga
    19141 ttgccgacaa gccattattt actcttgatg aacgcgagct aaagcgcaat gccccctctt
    19201 acactgcgca aacactgaaa gagtggcggc aggagcaagg accggacgtg ccgctggcgt
    19261 ttattattgg tcaggattca ttgctgacct ttccgacctg gtacgaatac gaaacgatac
    19321 tcgacaatgc acatttgatc gtctgtcggc gtcctggtta cccacttgaa atggcacaac
    19381 cacaatatca gcaatggctg gaggatcatt tgacacataa cccggaagat cttcaccttc
    19441 agcctgccgg taaaatttat ctggctgaaa cgccgtggtt taacatctcg gcgaccatca
    19501 tccgcgaacg tttgcaaaat ggtgaatcgt gtgaggattt attaccggaa ccggtactga
    19561 cttacattaa ccaacaaggc ttgtatcgct gatacctgtc gttacggagg gcatgatgcg
    19621 actgtggtta attcgtcatg gtgaaacgca agcgaatgtc gatggtctgt acagcggtca
    19681 tgcgcccaca cccctgaccg cgcgcggtat tgagcaagcg caaaatctgc atacgctgct
    19741 acatgatgtt tcctttgacc tggttttatg cagtgaactg gaacgggcac agcataccgc
    19801 gcgactggtt cttagtgacc gccagcaccc cgttcatatc atccctgaac tcaatgaaat
    19861 gttttttggc gactgggaga tgcgacatca tcgtgacctc atgcaagaag atgccgaaaa
    19921 ctatagcgcg tggtgcaatg actggcagca tgctatcccc acgaacggtg aaggatttca
    19981 ggcattttcg caacgtgtgg aacgatttat cgcaaggctt agtgaatatc agcactatca
    20041 gaatatttta gtcgtcagcc atcagggcgt actgagtctg ttaattgctc gtttaattgg
    20101 catgcctgcc gagtctatgt ggcatttccg cgttgaccag gggtgttgga gcgccattga
    20161 tatcaaccaa aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa
    20221 tgcatgactt ttctgttttt ttacgggtaa gccgcaacga ccattgacag ccccgggcag
    20281 gctgatattc tccgcagcca gactttttcc gccagacacg actttgtaga aattgtttta
    20341 caaaaatggc gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga
    20401 ttcggcgaca attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat
    20461 ctatttgatt cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg
    20521 acaaaattga tgacctcaaa ggtcaggaca tcatcgcctt agacgttcag ggcaaatcca
    20581 gcatcaccga ctgcatgatc atctgtacgg gtacgtccag ccgccatgtt atgtccattg
    20641 ctgatcacgt tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg
    20701 aaaacagcgc cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg
    20761 aagagagccg tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac
    20821 ttgtcgccgt gggaacgaaa atgccggact gggtacaaac cggttttacc gagtacctgc
    20881 gtcgttttcc gaaagatatg cccttcgagc tgattgaaat tccggccgga aaacgcggca
    20941 agaatgcgga catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag
    21001 gcaaaaaccg cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag
    21061 ccgctgagct ggaacgctgg aagctggatg gtcgcgacgt cagtctactg attggcgggc
    21121 ctgaagggtt gtcgcctgcc tgtaaagcgg cggcggagca gagctggtcg ctgtcggcac
    21181 ttacccttcc ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga
    21241 gcatcaccac caaccatcct tatcaccgtg agtgataagt gagctttgag tagaaaacgc
    21301 agcggatgaa actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc
    21361 gccgggcgct ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc
    21421 tgtataatct gcaaattgtt cgctttaccg actaccagac ccgctctaat gaaaaccgca
    21481 ttaagctggt gcctatcgca cccagccgcg gcattatcta cgaccgtaac ggtatccctc
    21541 tggctctcaa ccgcactatc taccagatag aaatgatgcc ggagaaagtc gataacgtgc
    21601 agcaaacgct ggacgctttg cgcagcgtgg ttgatctgac cgatgatgat attgcggcat
    21661 tccgaaaaga gcgcgcacgt tcacaccgtt tcacctctat tccggtgaaa actaacctga
    21721 ccgaagtaca agtagctcgc tttgccgtca accagtaccg ttttccgggt gttgaagtta
    21781 aaggctataa acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg
    21841 tgtcgaaaat caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact
    21901 atgcggcaac gcatgatatc ggtaagctgg gcattgaacg ttactatgaa gacgtgctgc
    21961 acggtcagac cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt
    22021 taaaagaagt accaccgcaa gccggacacg atatttacct gacgctggat ctcaaactcc
    22081 agcaatatat tgaaacgctg ctggcgggta gccgcgcagc tgtggtagtc accgatccgc
    22141 gtacaggtgg ggtgttggcg ctggtttcca cgcctagtta tgacccaaac ctgtttgttg
    22201 acggtatctc cagcaaagat tattccgcct tgctgaacga cccgaataca ccgctggtga
    22261 accgcgccac acagggggtt tatcctcccg cgtctacagt taaaccctat gttgcggttt
    22321 cggcattgag tgccggggtg atcacgcgca atacgacgct gtttgacccc ggctggtggc
    22381 aactgccagg gtcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga
    22441 atgtcacaag atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata
    22501 tggggatcga tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta
    22561 tcgacctggc ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct
    22621 ttaaaaaacc gtggtatcag ggtgacacca ttccggttgg tatcggtcag ggttactgga
    22681 cagcgacccc aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga
    22741 aggttccgca tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacaac
    22801 cgcatgaacc gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta
    22861 tgtacggtgt tgctaaccgc cctaacggta cggcgcataa atattttgcc agcgcaccgt
    22921 acaaaattgc ggcgaaatcc ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct
    22981 ataatgcgca caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc
    23041 catacaacaa tccgcaagtg gctgtcgcca tgattctgga gaacggtggc gcgggtccgg
    23101 cggttggtac gctgatgcgc cagatcctcg accacattat gctgggtgat aacaacaccg
    23161 atctgcctgc ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacagataa
    23221 tccgaataaa aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatctt
    23281 actggcattg ctggtttaca gcgccctggt tatctggagc gccagcggtc aggatattgg
    23341 catgatggag cgtaaaatcg gccagattgc gatgggtctg gtcatcatgg tggtgatggc
    23401 gcaaattcct ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat
    23461 tttgctggtg gcggtagatg ctttcggtgc catctctaaa ggcgctcaac gctggctgga
    23521 cctcggtatt gttcgttttc aaccgtcgga aattgccaag atagccgtac cgttgatggt
    23581 tgcacgcttt atcaaccgcg acgtttgccc accttcattg aagaatacag ctatcgcgct
    23641 ggtgctgata tttatgccca cgctgctggt ggctgcacag cctgacttgg gaacatcaat
    23701 cctcgttgcg ctttccggcc tgtttgtact gttcctctct ggccttagct ggcgtctgat
    23761 tggcgtcgca gtagtgctgg tagcggcgtt cattccgatt ctgtggttct ttctgatgca
    23821 tgattaccag cgccagcgcg taatgatgct cctggacccg gaatcagacc cactcggcgc
    23881 aggctatcac attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg
    23941 ctggctgcac ggcactcagt cacagcttga atttctcccc gaacgccata ccgactttat
    24001 cttcgcggta ctggcggaag agctgggatt agtgggcatt ttgattctgc tcgctctcta
    24061 cattctgctg atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg
    24121 cgtcatggct ggcggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat
    24181 ggtaagcggt attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc
    24241 ggcgctaatt gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa
    24301 aatgttgtcg aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca
    24361 tcgcggcagg aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg
    24421 taccgcagcc tgcggtatgt aacggcccca tagttgaaat tagcggggca gacccgcgtt
    24481 tcgaaccact gaacgcgacg gcaaatcagg attaccagcg cgacgggaaa agctacaaaa
    24541 tcgtgcagga tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac
    24601 ccggcagtaa cctgacggcc tctggcgaag ctttcgatcc gacacagttg acggcggccc
    24661 atccaacgct tccaatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga
    24721 tcgtggtgcg cattaatgat cgcggccttt atggcaacga ccgcgtgatt tcgctttctc
    24781 gcgcggcagc tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta
    24841 ttgtcgccca ggatggttcg ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac
    24901 agacttacgc cctgccagca cctcccgatt taagcggtgg cgcgggaaca agttcagtgc
    24961 ctgtcccgca gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga
    25021 ccggcgcgcc ggtaaccagc agcggtttcc tcggcgcacc aacgacctta gcgcctggtg
    25081 tactggaagg cagcgaaccg acgcctgcgc cacagcccgt tgttacagct ccgtcgacaa
    25141 cgcctgcaac ctcacctgca atggtgacac cgcaagccgc ctcgcaaagc gccagcggca
    25201 actttatggt gcaagtcggg gccgttagcg atcaggctcg tgcgcaacag taccaacagc
    25261 aactgggaca gaagttcggc gtccccggtc gcgtaactca aaatggcgcg gtctggcgga
    25321 tccagcttgg cccattcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa
    25381 ccgaagccca attacagtca tttattacca ccgcgcagta gcgtaaagca ggcatctgat
    25441 gtgtcaattt ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta
    25501 gtgcatttgc tatagtaggg cacttttttt aattccatca cggatgtcgt agttcagacc
    25561 atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc
    25621 acagccttta tctctgctgc acatgccgat gacctgaata tcaaaaccat gatcccgggt
    25681 gtaccgcaga tcgacgcgga gtcctacatc ctgattgact ataactccgg caaagtactc
    25741 gccgaacaga acgctgatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt
    25801 tacgttatcg gccaggcaat gaaagccggt aaatttaaag aaactgattt agtcactatc
    25861 ggcaacgacg catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc
    25921 aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt
    25981 aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc ttttgttggc
    26041 ttgatgaaca gctacgttaa cgccctgggc ctgaaaaata cccacttcca gacggtacac
    26101 ggtctggatg ctgatggtca gtacagctcc gcgcgagata tggcgctgat cggccaggcg
    26161 ttgatccgtg acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac
    26221 ggtattcgcc agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc
    26281 atcaaaaccg gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc
    26341 cagatgcgct tgatctctgc ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa
    26401 agtaaaaaat tgctaacctg gggcttccgt ttcttcgaaa ccgttaatcc tctgaaagta
    26461 ggtaaagagt tcgcctctga accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg
    26521 gttgataaag acgtgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc
    26581 tatgtgctga acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact
    26641 atcaacttcc agcttgatgg caaaacgatc gaacaacgcc cgctggttgt gctgcaagaa
    26701 atcccggaag gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac
    26761 tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc gtccccatat actaagcatc
    26821 agtaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg ccggagctga
    26881 catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt acaaagttat
    26941 ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc gccatgcgcc
    27001 aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact cggtatctat
    27061 cactatcaac gccactcata tcgagcaggt tgaaacactg tatgaagaac tgggcaaaat
    27121 cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggctcct ggtcgggtaa
    27181 ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc cacggagatg
    27241 ccgttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct tacgagccaa
    27301 tctcccaggc tatgcatgaa ttcaccgata cccgcgatga tagtaccctt gatgaaatct
    27361 ggctggtcga gcactatccg gtattcaccc aagggcaggc aggaaaggcg gagcacattt
    27421 taatgccggg tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg acttatcacg
    27481 ggccggggca acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa ctcggtgtgc
    27541 gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa ctgggtatag
    27601 aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa atttgctcac
    27661 tgggtttacg tattcgacgc ggttgttcat tccacggtct ggcattaaac gtcaatatgg
    27721 atctttcacc atttttacgt attaatcctt gtgggtatgc aggaatggaa atggctaaaa
    27781 tatcacaatg gaaacccgaa gcgacgacta ataatattgc accacgttta ctggaaaata
    27841 ttttagcgct actaaacaat cctgacttcg aatatattac cgcttaattc catacatcaa
    27901 tggcccaatt tacactgggt cattactcat aatacctgct aatgctttac atctccttcg
    27961 cgtttgtata ttttctttat cacttgcaga aatgcgatgc tacgccttgc tccgtagccc
    28021 acctaattga ttaacccatt gaaataattg accatggtga gcaaaagcgt
//
LOCUS       DAZUCC010000042        27855 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZUCC010000042 DAZUCC010000000
VERSION     DAZUCC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.042"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(35..1318)
                     /gene="yeaH"
                     /locus_tag="ACHY09_001616"
     CDS             complement(35..1318)
                     /gene="yeaH"
                     /locus_tag="ACHY09_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFS3884601.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1431..3365)
                     /gene="yeaG"
                     /locus_tag="ACHY09_001617"
     CDS             complement(1431..3365)
                     /gene="yeaG"
                     /locus_tag="ACHY09_001617"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFS3884602.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3801..4547
                     /gene="mipA"
                     /locus_tag="ACHY09_001618"
     CDS             3801..4547
                     /gene="mipA"
                     /locus_tag="ACHY09_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFS3884603.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYDGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4637..5491
                     /gene="yeaE"
                     /locus_tag="ACHY09_001619"
     CDS             4637..5491
                     /gene="yeaE"
                     /locus_tag="ACHY09_001619"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFS3884604.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYAEGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQIPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5542..6444)
                     /gene="yeaD"
                     /locus_tag="ACHY09_001620"
     CDS             complement(5542..6444)
                     /gene="yeaD"
                     /locus_tag="ACHY09_001620"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFS3884605.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGPAAQQGLPAHG
                     FARNLPWTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKACEIDLEAH
                     GEFETTSALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYL
                     NPQDCSVINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAY
                     ASETQKVTKEKPAHLAQSIRVAKR"
     gene            complement(6528..7523)
                     /gene="gapA"
                     /locus_tag="ACHY09_001621"
     CDS             complement(6528..7523)
                     /gene="gapA"
                     /locus_tag="ACHY09_001621"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS3884606.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7865..8278
                     /gene="msrB"
                     /locus_tag="ACHY09_001622"
     CDS             7865..8278
                     /gene="msrB"
                     /locus_tag="ACHY09_001622"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS3884607.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8320..8592
                     /gene="yeaC"
                     /locus_tag="ACHY09_001623"
     CDS             8320..8592
                     /gene="yeaC"
                     /locus_tag="ACHY09_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFS3884608.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8753..9394)
                     /gene="pncA"
                     /locus_tag="ACHY09_001624"
     CDS             complement(8753..9394)
                     /gene="pncA"
                     /locus_tag="ACHY09_001624"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFS3884609.1"
                     /translation="MHPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(9405..10421)
                     /gene="ansA"
                     /locus_tag="ACHY09_001625"
     CDS             complement(9405..10421)
                     /gene="ansA"
                     /locus_tag="ACHY09_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS3884610.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(10586..12442)
                     /gene="sppA"
                     /locus_tag="ACHY09_001626"
     CDS             complement(10586..12442)
                     /gene="sppA"
                     /locus_tag="ACHY09_001626"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS3884611.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            12603..13154
                     /gene="ydjA"
                     /locus_tag="ACHY09_001627"
     CDS             12603..13154
                     /gene="ydjA"
                     /locus_tag="ACHY09_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFS3884612.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAAMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            13271..14314
                     /gene="selD"
                     /locus_tag="ACHY09_001628"
     CDS             13271..14314
                     /gene="selD"
                     /locus_tag="ACHY09_001628"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFS3884613.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRHACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRELLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            14319..16274
                     /gene="topB"
                     /locus_tag="ACHY09_001629"
     CDS             14319..16274
                     /gene="topB"
                     /locus_tag="ACHY09_001629"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS3884614.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(16351..17694)
                     /gene="gdhA"
                     /locus_tag="ACHY09_001630"
     CDS             complement(16351..17694)
                     /gene="gdhA"
                     /locus_tag="ACHY09_001630"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS3884615.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            17930..18202
                     /gene="ynjH"
                     /locus_tag="ACHY09_001631"
     CDS             17930..18202
                     /gene="ynjH"
                     /locus_tag="ACHY09_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="HFS3884616.1"
                     /translation="MSRALFAVVLAFPIIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQQDDKNLSTNPLVWRRVKP"
     gene            complement(18168..18575)
                     /gene="nudG"
                     /locus_tag="ACHY09_001632"
     CDS             complement(18168..18575)
                     /gene="nudG"
                     /locus_tag="ACHY09_001632"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFS3884617.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            18662..19282
                     /gene="ynjF"
                     /locus_tag="ACHY09_001633"
     CDS             18662..19282
                     /gene="ynjF"
                     /locus_tag="ACHY09_001633"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS3884618.1"
                     /translation="MLDRHLHPRIKPLLHQCVKVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALIVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(19291..20598)
                     /gene="ynjE"
                     /locus_tag="ACHY09_001634"
     CDS             complement(19291..20598)
                     /gene="ynjE"
                     /locus_tag="ACHY09_001634"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFS3884619.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSANWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKKRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDIEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(20665..21318)
                     /gene="ynjD"
                     /locus_tag="ACHY09_001635"
     CDS             complement(20665..21318)
                     /gene="ynjD"
                     /locus_tag="ACHY09_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS3884620.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGGLAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(21318..22853)
                     /gene="ynjC"
                     /locus_tag="ACHY09_001636"
     CDS             complement(21318..22853)
                     /gene="ynjC"
                     /locus_tag="ACHY09_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS3884621.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAVLTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQAKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLTATFIALILIL
                     AWLEWGSSRRHFWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGYLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(22826..23992)
                     /gene="ynjB"
                     /locus_tag="ACHY09_001637"
     CDS             complement(22826..23992)
                     /gene="ynjB"
                     /locus_tag="ACHY09_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS3884622.1"
                     /translation="MRHCVWLLGLLSLFSLATHASDWQEIKNDAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYDINLKIVHLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQFLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(24002..24550)
                     /gene="ynjA"
                     /locus_tag="ACHY09_001638"
     CDS             complement(24002..24550)
                     /gene="ynjA"
                     /locus_tag="ACHY09_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS3884623.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SIFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLVTHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(24550..25257)
                     /gene="ydjZ"
                     /locus_tag="ACHY09_001639"
     CDS             complement(24550..25257)
                     /gene="ydjZ"
                     /locus_tag="ACHY09_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFS3884624.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(25271..25948)
                     /gene="ydjY"
                     /locus_tag="ACHY09_001640"
     CDS             complement(25271..25948)
                     /gene="ydjY"
                     /locus_tag="ACHY09_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFS3884625.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(25953..26663)
                     /gene="ydjX"
                     /locus_tag="ACHY09_001641"
     CDS             complement(25953..26663)
                     /gene="ydjX"
                     /locus_tag="ACHY09_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFS3884626.1"
                     /translation="MNAERKFLFACLIFALVIYAFHAFGLFELLTDLPHLLTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            complement(26830..27636)
                     /gene="xthA"
                     /locus_tag="ACHY09_001642"
     CDS             complement(26830..27636)
                     /gene="xthA"
                     /locus_tag="ACHY09_001642"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS3884627.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTANRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         6866 a         7092 c         7194 g         6703 t
ORIGIN      
        1 aatcagccag gcgataatga cccggctgac agttttagtc tttagctgtt gcattttgtt
       61 tatgaaacag ttcacggaac accggataaa tatcatcctg gtcgcggata tgctgcatcg
      121 caaagttgtc gaaagtagat tgcagatgct catattctcg ccacagtgtc tgatgcgcgc
      181 gacgtgtaat ttcgatatag ctgtaataac gaacaacggg taatattttt ttcgccagaa
      241 tttcatggca aagcggagag tcatcggccc agttatcgcc gtctgatgct tgggctgcgt
      301 aaatattcca ctgtgccggg ttataacgct ctttcactac ctcatccatc agtttcaggg
      361 cgctggaaac aatggtgccg cctgtttcct gcgagtagaa aaactcatgt tcatcgactt
      421 ctttcgcctg ggtatgatgg cggatgtata cgacttccac gttcttatat gttctgctga
      481 ggaacagata cagcagaata taaaaacgtt tagccatatc tttagtggac tgatccattg
      541 aaccggaaac gtccatcagg caaaacatca ctgcctggct ggagggatcg ggccgttttt
      601 cgtagttctt gtaacgtaaa tcgaaggtgt caatgaaagg gacgcgttca attttggcac
      661 gtaattctgc aatttctttg cgcagacgtt cctcttccag cagttgcgca ggttcactgt
      721 tgctgatgat ggccaaattc tcttccagcg catgaagttc ccgccgcttg cccgccgtca
      781 tggctgtgcg ccgcgccagt gagttctgca atgaacgcac aacgctgata ttggccggaa
      841 cgccgttagc ggtataaccc gcccgatgcg ttttatattc ggtcagctgg cgttgttggt
      901 tttgtttcag attcggtaaa gccaaatctt caaagagcag atcaagatac tcatctttcg
      961 aaatctgaaa gacgaattca tcctgacctt caccatcctg gctggcctgg ccctgaccgc
     1021 tgccggaacc tccgccgcca ccctggggac gttcaattcg gtcgttctgg acgaaatggt
     1081 cattgcccgg atgcacgcgg tggcgcagac cgccacgccc ctgatgaaac atcggttcgc
     1141 taatatcttc cgtgggaatg gatacggact cgccgctgtc gacgtcagtc accgaacgct
     1201 tattaatggc ctcggagatc gactgtttaa tttgcgcttt ataacggcgt aaaaaacgct
     1261 ggcgattcac catgcttttg tttttgccgt tcagacgccg gtcaataaac caggtcatat
     1321 gccccccgta ctacatttgc caactttatg ctacgcctgt gttgccgctt ctctggccca
     1381 cggttcgctg tcgttgtagg cccggtagca tgtgtaccgg gccagggttg ttaagacgat
     1441 ttacgtacgc gcaaatacca ttcgcacagt aaacgcacct gtttacgggt gtagcctttt
     1501 tccatcatac ggtcgacaaa gtcgtcatgt ttcttttgct cgtcggttga cgttttggcg
     1561 ttaaacgaaa taaccggcaa cagctcctcg gtattggaga acattttctt ctcaataacc
     1621 gtgcgcagtt tttcatagct ggtccagttc ggattgcgtc cgctgttatt cgctctggcg
     1681 cgcagtacga agttaacaat ctcgttgcgg aaatcttttg gattactgat ccccgccggt
     1741 ttctcgattt tctccagctc ggcgttaaga gactcgcggt caaacaactg cccggtatca
     1801 ggatcgcgat actcctgatc ctgaatccag aaatccgcgt aggtaacata acggtcgaaa
     1861 atgttttgcc catattcgga ataggattca aggtaggccg tctggatctc tttgccgata
     1921 aactcggcat atttcgggat cagataacct ttcaggaact ccagatagcg ttctgcctgt
     1981 tcttgcggga actgctcgcg ctcgatctgc tgttccagga cgtagaacag atgaaccggg
     2041 tttgccgcca cttctacatg atcgaagttg aacacgcggg agaggatctt aaacgcaaaa
     2101 cgcgtcgaca gaccgttcat cccttcatcg acaccggcgt agtcacgata ttcctgatac
     2161 gacttggctt tgggatctgt gtctttcaga ctttcgccat cataaacccg catctttgaa
     2221 taaatgctgg agttttctgg ctctttaagg cgcgaaagaa tggaaaaacg tgacagcgtt
     2281 tccagcgtgc caggggcgca gggggcgtga gtcaattcac tgtgattaag caatttctcg
     2341 tagattttga tctcttcgga aatgcgcaag caatacggca ccttcacgat gtaaacacga
     2401 tcgaggaagg cttcgttgtt tttgttatta cggaaagtga cccattcgga ttcgttcgag
     2461 tgggcgagaa taatcccgtt gaacggcagg gcggagatac cttccgtccc gttgtagtta
     2521 ccttcctgag tggcggttag caagggatgc agcactttaa tcggtgcttt aaacatctca
     2581 acgaattcca tgatcccctg gttggcgcgg cacagtgcac cggaatagcc gtaggcgtcc
     2641 gggtcattct gcgcgtagtg ttcgagttta cgaatatcga ctttcccaac cagcgcggag
     2701 atgtcctggt tgttctcatc accgggttcc gttttggcga tagcaatttg ttgcagaatt
     2761 gacggccaga ccttcactac ccggaactta gtgatatcgc caccaaattc atgcaggcgt
     2821 tttgccgccc acggcgacat gatggtgccg agataacggc gaggaatgcc atactctttt
     2881 tccagaatct gcgcatcttc ctgcggattg aaaagacaga acggatgatc gttgaccggg
     2941 ctacgctcac cgttggcgct caatacataa atcggtacga gctgcattaa tgatttcagt
     3001 cgctcagcaa gcgatgattt acccccaccc acaggcccaa gcagatacag gatttgtttc
     3061 ttctcttcca gcccctgagc cgcgtgtttc agataagaga caatctgttc aatcgcgtct
     3121 tccatgccgt aaaactcttc aaatgccgga taacgtgcta tgacccggtt agaaaagagt
     3181 cgagaaagtc tgggttcctg ggctgtatcg accatgacag gctcaccgat agccatcaat
     3241 agccgctcag ccgcgttggc ataagcactg cgatcttgcc gacaagtggt aagaaactcc
     3301 tgcagtgtga actcttcgtc cttggcagct tcatatcgct ggcgatagtg atcgaatata
     3361 ttcatggtat gccgtccttt cgttttttag cacaggttaa gagccgttca tatgaatagt
     3421 agaggctccc ggaagaagta aactgaacga cgtctcttgc taaaacacag caactctcat
     3481 gccattttat cgacgatgag aacaacgtgc ccggtgatac accttcaaaa ttaagcgtag
     3541 atggcaaatg gaaaacttgc ctgcacggct ttcttaattt caatggcata tcaacaactc
     3601 atccataaat tttgcataat taatgtaaat accagggtcg ccggtaacgc taaattcatt
     3661 tggctgtaag agtggtgtca tctgcgtcag gaaaattaaa cagttactat aaaaaatgaa
     3721 aacgtaaaaa ggttgggttt cgatgtattg acgggtaaac tttgtcgccc gctaaacatt
     3781 tgtttaagga atgattaatt gtgaccaaac tcaaacttct ggcacttgga gtgcttatcg
     3841 caacgtctgc aggcgtagcg cacgctgaag gtaaattttc cctgggcgca ggcgtaggtg
     3901 tcgttgaaca cccatataaa gattacgata ccgatgttta cccagtaccg gtaatcaact
     3961 atgacggcga taacttctgg ttccgtggct taggtggtgg ttactacctg tggaatgacg
     4021 caacggacaa actttcaatt accgcttact ggtcgccgct ttacttcaaa gctaaagaca
     4081 gtggcgatca ccaaatgcgt cacctggatg accgtaagag caccatgatg gctggtctgt
     4141 cttatgctca ctttacccag tacggttacc tgcgtaccac cctggctggc gataccctgg
     4201 ataacagcaa cggcatcgtc tgggatatgg cctggttgta tcgttacacc aacggtggcc
     4261 tgaccgtgac tccgggtatt ggtgtgcagt ggaacagcga aaaccagaac gaatactatt
     4321 atggcgtatc gcgcaaagag tccgctcgca gcggtctgcg tggctataac ccgaacgaca
     4381 gctggagccc ttacctggag ctgagcgcca gctacaactt cctcggcgac tggagtgttt
     4441 acggtaccgc gcgctacacc cgtctgtctg atgaagttac tgacagccca atggtggata
     4501 aatcctggac tggcctgatt tctaccggga tcacctacaa attctgataa tgcatctttc
     4561 cagggcattt tgtgcataaa aacagggcgt catgccctgt tttgcattac ggtggtgcaa
     4621 tacctgggag gaaacaatgc aacaaaaaat gattcaattt agtggcgatg tctcactgcc
     4681 agccataggg cagggaacat ggtatatggg cgaagatgcc agtcagcgca aaacagaagt
     4741 tgctgcacta cgcgcgggca ttgaactcgg tttaaccctc attgataccg ccgaaatgta
     4801 tgccgaaggc ggtgctgaaa aggtggttgg ggaagcatta accggtctgc gtgataacgt
     4861 ctttctcgtc tctaaagtct atccgtggaa tgctggcggg caaaaagcga taaatgcatg
     4921 cgaagccagt ttacgccgtc tcaatactga ttatctcgat ctctacttat tacactggtc
     4981 tggcagtttc gcttttgaag agactgtcgc agcgatggaa aaattgatcg cccagggaaa
     5041 aatccgccgc tggggcgttt ctaaccttga ttatgccgac atgcaggaac tctggcagct
     5101 gccgggggga aatcagtgtg ccactaatca ggtgctttac catctcggtt cacgagggat
     5161 tgagtacgat ctactcccct ggtgccagca acagcagata ccggtgatgg cttacagtcc
     5221 gttagctcag gccgggcggt tgcgcaatgg gctgctaaaa aacgcagtag tcaacgaaat
     5281 tgcacatgct cacaatatca gcgcggcaca agtattgttg gcgtgggtga tcagtcatca
     5341 gggtgtgatg gcgattccaa aagcggccac gattgcccat gtccaacaaa atgcggctgt
     5401 gcttgaggtt gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     5461 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg caacgcaccc
     5521 ggcacacaac attaacgcaa attaacgttt cgcaacgcga atggattgcg ccagatgtgc
     5581 aggtttttct ttggtcactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     5641 ggttttgtag ccatcgtccg gcatatcgcc catgctaact gaaagcgccg gtcctgggtt
     5701 ccagccaacg acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     5761 gtcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     5821 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     5881 atcgcccagt ccactgacgc ttaccttagt gatatcaccc acgttaaagt aggtatgcag
     5941 ggcagaggtg gtttcaaatt cgccgtgtgc ttcaagatcg atttcacagg ctttacccac
     6001 gcggaaatgc gctaatagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     6061 tgtcaattca aaagtcagcg ctacgccatc agcatcttca cgatgtgatt tcagcgtcca
     6121 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgcagccg gaccttgttg
     6181 tgcagccgga ccaaaccacg gccagcaaac cggtacgcca ccgcgaatag cgacgccatt
     6241 tttgaacggt gtgttattgc tcaaccacag aacttcttct tcacccgcag gtttccatga
     6301 gagaaggtgt gcgccctgta atgcaaaaga ggcttttacc tggggatgat cgaccacaat
     6361 gaggtccagt tcatccagtt tacgacggga gaggacaggg gagatttgtt cgatgaccgg
     6421 aagggcaaaa attttcttaa tcatgacgca gtcctttaac ttcattttat caggtaaaaa
     6481 aaagagcgac cgaagtcgct ctttttagat cacagtgtca tctcaactta tttggagatg
     6541 tgagcgatca ggtccagaac tttgttggag taaccggttt cgttgtcgta ccaggatacc
     6601 agtttcacga agttgtcgtt cagagcgata ccagctttag catcgaacac ggaagtgcaa
     6661 acttcgccgt tgaaatcggt agatactacg tcatcttcgg tgtagcccag aacgcctttc
     6721 atttcgcctt cagcagcagc tttaacggca gctttgatct gctcgtaagt tgcagctttt
     6781 tccagacgaa cggtcaggtc aactacggat acgttcgggg tcggaacgcg gaacgccata
     6841 ccagtcagtt tgccattcag ttctggcagt actttaccta cagctttagc agcaccggta
     6901 gaggacggga tgatgttctg ggaagcaccg cggccgccgc gccagtcttt gtgagacggg
     6961 ccatcaacgg ttttctgagt agcggtagta gcgtgaacgg tggtcatcag accttcgatg
     7021 atgccgaagt tatcgttgat aactttagcc agcggagcca ggcagttggt ggtgcaggaa
     7081 gcgttggaaa cgatgtcctg gccagcatat ttgtcgaagt tagcgccttt aacgaacatc
     7141 ggagtgttgt ctttagacgg accagtcata accactttct tcgcgccagc ggtgatgtgt
     7201 ttacgagcag tttcgtcagt caggaacaga ccagttgctt cagcgacaac gtcaacacca
     7261 acttcgtccc atttcaggtt agccggatca cgttcagcgg taacacggat ttttttaccg
     7321 ttaacgatca gatgaccgtc tttcacttca acggtaccgt cgaaacggcc gtgagtggag
     7381 tcatatttca gcatgtatgc catgtaatca gcgtctaaca ggtcgttgat tgcaacgatc
     7441 tcgatgtcag aacgtttctg agcagcacgg aaaacaatgc gaccgatacg gccaaaaccg
     7501 ttgataccta ctttgatagt catatattcc accagctatt tgttagtgaa taaaaggttg
     7561 cctgtaaaat tacaaaaacc ttacgcagcg tcaagcggaa tcgtgtcaat cattgcgaca
     7621 aatcaatcct gtgcctaagc attacgcgac tgactcgcct cactcttcct ttgggcttga
     7681 gaccacatgg ggtcggcgcc ccgaatttta aagggcaatt acgataaaaa tgtgattttc
     7741 atcacgattt aggtgcagcc atttcgtcga gatcaagttt aggacaaaat tttgccagtt
     7801 ggtgttaatg ttttgttaga atcagtcacg taatgtgagc acgattaaag tgagatgtga
     7861 gcaaatggct aataaacctt cggcagaaga actgaaaaaa aatttgtccg agatgcagtt
     7921 ctacgtgacg cagaatcatg ggacagaacc gccatttacg ggtcgtttac tgcataacaa
     7981 gcgtgacggc gtatatcact gtttgatctg cgatgccccg ctgtttcatt cccaaaccaa
     8041 gtatgattcc ggctgtggct ggcccagttt ctacgaaccg gtgagtgaag aatctattcg
     8101 ttatatcaaa gacttgtcac atggaatgca gcgcatagaa attcgttgcg gtaactgcga
     8161 tgcccatctg ggccatgtct tccctgacgg gccgcagcca acgggcgaac gttattgtgt
     8221 taactctgct tctttacgct tcactgatgg cgaaaacggc gaagaaatca acggttgaaa
     8281 aaacgattca gcaacttatt ccacaggagc ggaaaaatta tgaatcttga tgacattatc
     8341 aacagcatga cgcctgaggt ataccagcgt ttgtcgaccg ccgttgaact ggggaaatgg
     8401 cctgatggcg ttacgttgac cgaggaacaa aaagagaact gcctgcaact ggtgatgctg
     8461 tggcaagccc gcaataatac cgaagcacag catatgacaa ttgacacaaa tggtcagatg
     8521 gtcatgaaga gcaaacaaca gttaaaagaa gattttggta tctcagcaaa gcctattgcc
     8581 atgtttaagt gaatgagttg tctagtattt aagcctgtta ttaagggagg gcaaatagac
     8641 ttgttcggat gtcgtgtttt agctgcgagt aggttaaatg caacactaac aagtattacc
     8701 tggcctacaa tgtcattgag gaaatgtagg ccagagatga atccaactaa tattactcct
     8761 gcgtctcttc ccaatctgcc agcgtatata gcgttgcccc agctgctgac atctccataa
     8821 acgcgtgcgc actgtcctgt ggctggatat tcacgccacg acaaccatcg gtaatcacat
     8881 taaccttata acctaactgt aacgcgtcca gcacggtaaa cttcacgcaa tagtcagtag
     8941 ccagacccat aatgatcaat tcatcgattt catgatcgcg taaccagtca tcgagcgtgg
     9001 ttttctgccg acggccgtta tcaaaaaagg cactgtaact gtcaactaaa ggattttcgc
     9061 ctttatggaa caccgctgcg atcgcttttt ggttcagtaa cggatgtaat tgtgcgcctt
     9121 cactgttctg cacacagtga tctggccaga aggtttgtgg caaaccgtcg agttggcctg
     9181 gcgtataagg ctctacaccg tgctgactgg caaaactgcc gtgattcgcc gggtgccagt
     9241 cctgactggc gataaccgct tcaccgcgcg actggcacca gtcaatcagg cggttagcga
     9301 catccaccgt actgtcacct tccggcacgg cgagcgcgcc accagcacag aaatcatttt
     9361 gtaaatcgac cagtaacagg gcgcgagggt gcattgcagt ctccttaatc atccggcgtc
     9421 agttcgccac gcaggttttg gctcatggcc ttgcgaatgg tttcagtatc aagttcctgg
     9481 ctaagcaggt aatgcagttt ggttagcgtg gcttctacag tcatatctgc accgccaata
     9541 acgccggcat gggcgagggc gttaccggtg gcataaccac ccatgttcac tttaccggac
     9601 atacattgtg tcaggttgac caccacaata ccgcgatcgc tggcttcttg taattcctgc
     9661 aggaaggctt tgttttgtgg cgcattaccc acgccatatg agcgcagaat caatgctttc
     9721 acaggttggc gcagaaaatt gcgcacgacg tcagcagaaa tccccggata aatcgtcact
     9781 acgccaattg gttgcggcgt gattggatga acgatcaatt ccccttcacc gtgcggggcg
     9841 ggtggcgtat tcaaacggcg aatatggata cctgcttcca gtaacggagg aaggtttgga
     9901 gaggcaaacg catcaaaacc atcggcatgg gctttggtgg ttctgttgcc acgatacagt
     9961 cgattattaa aaaagagagt tacttcgttg ataggataat tcgccgcaac gtacaacgca
    10021 ttcagcagat taatttgtcc gtcagagcgt aactcagcca gcgggatttg tgaccctgtc
    10081 acaatgaccg gtttaccgag attctcgagc atgaacgaca gtgcagaggc ggtatacgcc
    10141 atcgtgtctg tgccgtgcag aatgacaaaa ccatcatagt cgtcatagtg cgctttaata
    10201 tcttcagcaa tatgctgcca gtcttctggc gtcatatcag aagaatccat caatggcgta
    10261 tattcatgaa tagtgaaatc tggcatctcc gggcgatgga attccggcat cagcgccagt
    10321 tggcgctgta gatgacctga caccggaata taaccctgct cggaacgctg catcccgatg
    10381 gtcccgcccg tgtaggcgac gtaaattgat ttcttttgca tgatattgat atgaatgata
    10441 tcgaaagagc aaaagtatat acgtgaggaa gggggagcgc acctgatact atgcggataa
    10501 ggaaatatgg ggcgagacag cgggaagagg attcgtcgag gtaaaaaaat gtggccgacg
    10561 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    10621 aacggttttg tgggtcgtta aacgctgcca gcttgtcgct ttcactttta acggtagagg
    10681 ctaccgaggc cagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    10741 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    10801 ccagatgcca ctgtttcact tttgccagct ctgctgcttt ggcgaccgca tcatcgaaat
    10861 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acatggccct
    10921 gggcgatttt atcaatctgc tctggcgttg aatgacgcgc atcagcaacc agcgtgataa
    10981 agcgtttata gccattctca atgcttaact gcatcatctg ctgcgcttcc ggcggcagtg
    11041 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    11101 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    11161 gggtgctggg gttagccaca atgtaattag ctggtgtgga aatccagtaa ccaccagatg
    11221 ccgccatgcc gcccatcgat acaaccacag gcttacccgc tgcccgggct gctgccagtt
    11281 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    11341 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    11401 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgactccga
    11461 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    11521 aatttttatc agtcttgctc cagccgaact ctttggtcag agttttttcg atttcggcac
    11581 tcgatgccag tgcatcgacc agcttgtttt ccagcgcata tttcgcggta tcgccaccgg
    11641 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    11701 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    11761 cagcttcgcg ggctgccggc gacatatcat cacgaataaa cggttcaacg gcagatttat
    11821 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagtaat gatttgtagt
    11881 acagaccgtt ggtggcaaag ccgtgcagat caaccacgcc ttgcggagac agccaaattt
    11941 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    12001 ccggtttccc gctatcacga aactctttca gagctttgcc gatgtactgc atagacggct
    12061 ggtcgccgcc tgcgaagttt ttcagatcca tcacaatacc ggtgatattg cggtcgtcct
    12121 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    12181 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtcg ggtttatcga
    12241 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    12301 caccactgac ctgcatccag atccccacac caaccagcac gagaaaaata aagaacaggt
    12361 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    12421 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tttcaggcgc
    12481 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactggg
    12541 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcatgaca ggagttaatc
    12601 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacctg
    12661 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggc tatgcgtgcg ccggaccata
    12721 agtccatgca accgtggcat ttctttgtga ttgaagggga agggcgcgag cgtttcagcg
    12781 ccgtactgga gcagggggcg attgctgccg gtagtgataa caaagccatc gacaaagcgc
    12841 gtaatgcgcc gttccgcgca ccgctcatca taacggtggt ggcgaaatgc gaagaaaatc
    12901 ataaagtccc gcgctgggaa caggaaatgt ctgcgggatg cgcggtcatg gcgatgcaaa
    12961 tggcagcagt tgcccagggg tttggcggca tctggcgcag tggtgcatta actgaaagtc
    13021 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    13081 tcggtacgcc gcagttgaaa gcatctacgt cgataaacgt cccggacccg acgccgtttg
    13141 tgacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    13201 cagacactct cacttatcac ttcatggaat gagggttacc atacccggat tgcattgaca
    13261 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gcgctggttg
    13321 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    13381 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tatacgatct
    13441 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    13501 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg caatgggcgg
    13561 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    13621 tgcccgcgaa gtgaccgaag gtggacgcca tgcatgccgt caggcgggga ttgcgctggc
    13681 tggcggtcac tccatcgatg cgccagagcc gatttttggt ctggcggtaa cggggatcgt
    13741 acctaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    13801 gaaacctctg gggattggcg ttcttaccac ggctgagaaa aaatcactgc tgaaaccaga
    13861 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    13921 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggt tttggtttac tggggcattt
    13981 aagcgaaatg tgtcagggcg caggtgtgca ggcgcgcgtc gactatgacg cgatcccgaa
    14041 attgccgggg gttgaagagt acattaagtt gggcgcagtg cctggcggca ccgaacgtaa
    14101 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg aactgctgtg
    14161 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    14221 caaagctaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    14281 gcgcggcggt cgtgccatgg ttgagatccg ttaattcaat gcggttgttt attgccgaaa
    14341 aaccgagtct ggcgcgcgcc attgctgatg tcctgcctaa accgcaccgg aaaggcgatg
    14401 gctttatcga gtgcggtaat ggtcaggtgg tgacctggtg tatcggtcac ctgcttgagc
    14461 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    14521 ttgtcccgga aaagtggcaa ttacagcccc gaccctccgt gactaaacaa cttaatgtca
    14581 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggagacccg gatcgcgaag
    14641 ggcaactgct ggtggatgaa gtgctggact acctgcaact ggcaccggaa aagcgccagc
    14701 aggtacagcg ttgtttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgatc
    14761 gtcttcgctc caatagcgag tttgtaccgc tgtgcgtttc tgcactggcg cgtgcgcgag
    14821 ctgactggct gtacggcatt aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    14881 gttatcaggg cgtgctttcc gtgggacgcg tgcagacgcc cgtactcgga ctggtggtgc
    14941 gccgcgacga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    15001 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    15061 cgtaccagga tgaagaaggg cgcttattac atcgtccact ggcggagcat gtggttaacc
    15121 gcattagtgg tcaaccagct attgtcacca gctataacga taaacgggaa tcagaatccg
    15181 cgccgctgcc tttttcgctt tcggcgttgc agattgaagc ggctaaacgt tttggtctga
    15241 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    15301 atccgcgttc cgattgccgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    15361 tgaatgctat cagcgttcat gcaccagatc tgttgccgca gccagtggtc gatccagata
    15421 tacgcaaccg ctgttgggat gacaaaaaag tcgatgcgca ccacgccatc atcccgaccg
    15481 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    15541 gtcagtattt gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    15601 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    15661 cactgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccgctg cctgtggtgg
    15721 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    15781 cgcgccattt taccgatgca acactgcttt cagcgatgac cgggatagca cgttttgtgc
    15841 aggacaaaga tctgaaaaag atccttcgtg cgaccgatgg tctggggaca gaagcgactc
    15901 gcgccgggat tattgaactg ctgttcaagc gaggcttcct gaccaaaaaa gggcgctata
    15961 tccactccac cgacgccgga aaagcgctat tccattcgct gccagaaatg gcgacgcgac
    16021 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    16081 atcaggactt tatgcagccg ctggtgggga cattgtacca gttgattgat caggcaaagc
    16141 gcacgtcggt aaggcagttt cggggaataa tggctcccgg cggtggagaa gggaagaaaa
    16201 aggattcgcc acgcaagaga gcgccgaaaa aaagcccgcc atcagaagag gcgggcaatg
    16261 gagtgataat ataaaggttc tttcaaccat ctgatactga agatttttat ggccggataa
    16321 ggcgtttgcg ctgtatccgg cacaattgtc ttaaatcacg ccctggctta acatcgcatc
    16381 cgcaaccttc acaaaaccgg caatgttcgc cccctgcacg tagttggttt gctcaccttc
    16441 accaccatgc tcaacacagg catggtggat atccagcatg atgtgatgca aacgtgcgtc
    16501 aactttctcg gctttccagc ccaggcgcgc agcgttttgc gccatttcca ggcccgatgt
    16561 tgctacaccg ccagcattag ccgctttacc cggtgcaaac agtacgcctg cctgctggaa
    16621 cagttcagtc gcttcgatgg tggtcggcat atttgcccct tcggcgacgg ctttaacgcc
    16681 attggcgata agctgatgcg cggcgtcaac atccagttcg ttctgggtgg cgcaaggcag
    16741 ggcgatatca accggtacag accacggttg ttggccttcg agatagacca gaccaaattc
    16801 tttggcgtaa tcagccactc gaccatcgcg gctggctttg atttcgataa gacgtgccag
    16861 tttctctttc gtgaatccgc tttcatcaac tacagtgccg ctggagtctg acgcagtgat
    16921 cacacgagca ccaaattcca tcgctttttc gatagcgtac tgggcgacgt tgccggagcc
    16981 agaaacggaa actcgcatcc cttcaaaacc cataccgtgg cgttttagca ttgcttcggt
    17041 gaaataaacc agaccgtagc cggtagcttc cgggcgaata agactgccgc caaatgaaag
    17101 gcccttaccg gtgaagacgc aggcggtatt gttggagagc tttttcatca tccccgccat
    17161 aaagccgact tcacgaccac caacgccgat atcacctgcc ggaacgtcag tatccgcgcc
    17221 caggtggcga tacagttcag tcatcagcgc ctggcaaaaa cgcataactt cgccttcgct
    17281 tttccctttc gggtcgaaat cgctgccgcc tttaccaccg cccatcggta gagtagtcag
    17341 ggcatttttg aaggtttgtt cgaagccgag gaatttgaga atggagaggt taactgacgg
    17401 atggaagcgc ataccgcctt tgtacgggcc gatggcagag ctgaactgca cacgccatgc
    17461 acggttgacc tgcacctggt tgcgatcatc aacccatact acgcgaaatt ggatcacgcg
    17521 ctccggttca accagacgct ccagtaatga catctgacga tatttgggat tttgttcaag
    17581 aaaaggccag agtgtggtca ttacttcacg aacggcttgc gcgaactcgg tttgattcgg
    17641 gtcgcgcttt tggacatggt tgaggaatga ctccagagaa tatgtctgat ccatagatat
    17701 aaaaccctta tatattaata cgattatgct tatgttgttt atgtcatgtg cttttgcagt
    17761 tttcgactat accatcaaga atgtgacggt aagcaagaaa aaaatgaccc aggaaagcaa
    17821 attaataacg agagtaatct cataaccaaa tgcgataact cagacgctaa agttttgcca
    17881 cccggctacc gttatcagta gcagagacaa taacgttaag gagttccctg tgagtcgagc
    17941 attgttcgcc gtagtattag catttccaat aattgcactg gctaacccgc attatcgtcc
    18001 ggacgtggag gtaaacgtac cgccagaagt gttcagttca ggcgggcagt cagcgcagcc
    18061 ctgtactcag tgctgcgttt atcaggatca aaattattct gaaggggcgg taattaaagc
    18121 cgaaggaata ctgctgcaat gccaacagga tgacaaaaac cttagcacta atccgctggt
    18181 ctggcggcgc gtaaagccat aaacgcctct aataatggaa tgtcagcagg ggccagcgga
    18241 tattgcagcg cctcttcagg tgagcaccag accagcgcct gatgttcatg tgcctgtaac
    18301 gtcccgtgga agtcgggtac gtgccaggca tgaagatgga taatccgccc cgaaacttct
    18361 cgctgatggc tggcaacata gtcaccaaca gttgcttcga tgcccagttc ttcgtttaac
    18421 tcacgcacca gcgcctgccg ctggctttca tccggctcga ctttaccacc ggcaaactcc
    18481 cataatcccg cctgatcgct ctgggcgggg cgttgcgcga gtaaaatttt gccatcacgt
    18541 tcaatgatgg cggcaacaac ttcaatcatt ttcataaatt acacacgggc agataagcga
    18601 aaaatgccat aatatccgca ctgttgctca atcggaaagg ctttatattt aaggagtgcc
    18661 tgtgctagac cgccatcttc atccccgaat taaacctttg ttgcatcagt gtgtgaaggt
    18721 gctggataaa cccggcatca cgccagatgg tttaacatta gtgggatttg ccatcggcgt
    18781 gctggcactg ccgtttctgg cgctgggctg gtatctggcg gcgctaatcg tcattttgtt
    18841 gaacaggctg cttgatggtc tggacggtgc gctggcgcgg cgcagaggac ttaccgacgc
    18901 gggcggcttt ctcgatattt ctctcgattt tctcttttac gcgctggtgc cgtttggttt
    18961 tattctcgcc gcaccagaac aaaacgcgct ggccggaggc tggctgttgt ttgcgtttat
    19021 cggcacgggc agcagttttc tcgcttttgc tgcgctggcg gcgaaacatc agattgataa
    19081 tcccggttat gcgcataaat cgttttatta tctgggcggc ttaaccgaag gcagcgagac
    19141 gattttgctg tttgttctga gttgcctctt tccggcctgt tttccgtggt ttgcgtgggt
    19201 atttggtgcg ttgtgctgga tgacgacctt tacgcgggtg tggagtgggt atctgacgct
    19261 gaagtcgctc cagcgtcagt aattgcaatg ttatttactg ctgtccgggc cgcgttcacc
    19321 ggttgctacc ggattttttg gatcgctgct ccattcgtac cagccaccgt cataaacgga
    19381 gacgttattc cagcccattg cgcgtgcgta cataaaggtt tctgacgcgc gccagccggt
    19441 gccgcagtag aatgaaactt gctgatctgg cttgatattc catgctttcc acatagcggt
    19501 aatatcatcg gcgctacgca tggtgccatc cgggttatgg aaatcttcca tatgcgtcga
    19561 gtcgctacca gcgtgtcccc aacgtgctcc ggctatttca ccttttggtt taatatagct
    19621 gtaaccgctg gtcgtaccga taaattctgg ccacgaacga atgctcacca gcgatgcatc
    19681 ctggcgatgc agcagtccac gcgcttgttc aatatcaagc atcagttgcg gttgtgccgg
    19741 gatcttcacg ccgaaatccg gttccgcttt cactttcggt ggcgttccgc gctcaacggg
    19801 cagtcccgcg tcggaccagg tttgccagcc gccgtccagc aggcggacat ctttcacgcc
    19861 agcgtagagc ataatttgtg ccacacgcgc tgcagcgtat acgtcacgcc cgtacagaat
    19921 gaccgtggtg tcatggcgaa tgccgtgttt tgccagcatc gcttttagtt gttcatcaga
    19981 aactttgttc cacagcggtt cgctttccac ttcgttggta tcgatgtagt cagcgccggg
    20041 aatatggctg ataaggtaaa gcttaggagc gccccaggcc gcttcaatga ctttccagtc
    20101 accggcaggt ttcgccgtaa cctctttacc ttgttgcagg tcgtgcagcc attgcggata
    20161 aaccagctgc tcaaaatgcg gcagtttttg cagacgggaa ggttcgctta gcgcgtcact
    20221 cagaatggag atatgcgtaa aacctgcttt ttgcagtcgc tttttgacgg cgtcgacatc
    20281 tttgtcatta ccgtacagcg ccaccggagc atcggctttc aggttatgtt gcttgatcca
    20341 ctcgttgagc tgttcggtgc tcattttgtc gagccagtta gcagagaggt ttaaggcggc
    20401 aggttcatga ccagaagggc catttaaggt ttgtggccag ccgttataaa acgcgctggg
    20461 gcgagtatct atcgctttgc cattttgttg ttgaagctgg tcaagtgtaa gaggcttcgc
    20521 cagttcagcg gcccacgaag aagcgcaagc cagccctaaa gccattgcca gcgcggtcat
    20581 ttgagaaaca cgtttcatcg aaaatcccga cattgtagtt ttgagtgcgc cattctgaac
    20641 tgacgcattg agaaaaactt tgcgttatcg cagtttgttg taattttctg accactgcgc
    20701 catatccaga acagaactat cagcaggaac atcctggaga tcgtgcgtta cctgaacgac
    20761 ggggatcgcc tgggcgcgaa cttcgctgaa cacccactgg cgaaaattat cgcgcagagc
    20821 cacatctaga cggctgaatg gctcatccag gagtaacgct tttggctggg cgagaagggc
    20881 gcgtagcaga gcaacgcgcg ctcgctgacc gccagacaaa gtagcaggat cctgatggaa
    20941 agctccgtca aggcctgaac gctcaagtgc atcattcacg gcattacgtc gggcattccc
    21001 tttaagtgtc gccggtagcg ccagcagtaa attttgcccg acgctgaact ggtcaaataa
    21061 cagtgcatcc tggaaaagaa tgccaatctg acgctgtgcg gtgggtagca tgtcaatccg
    21121 ttgctcattg agccatagct cccctgtaca agaaaactgt ccggccagtc caccaatcat
    21181 ccatgaaaac agagtggatt ttccacagcc agacggcccc attaacgtga caatgtcacc
    21241 tttatccacc gtaaagttaa cgtttgtcag caagcggctt tctggtaaac gtagcgaaac
    21301 atttttcacg cagagcatta gcggagtcct tgtctgacat aacctaccca ttttgcgact
    21361 aacgcggtca gggcaaaaat aataagcggt aatagcagtt gccataaagc ctgggcggcg
    21421 agaatgccgt tgctgccgcc gctgcttaat gccaccgctt cagtggtgag cgtcggaaaa
    21481 cgccccgcgc ccagccacag cgtcggcata tactgtgcaa tactgactga aaatcccacc
    21541 gcgaaggcaa tcagcgtagg gcgtagcata agcggacatt tcacgtaaaa gaagattttg
    21601 gaccgcgacc agcccagtgt ttgcgcaatc aaaattaacc gtgaatcaat gcgctgccag
    21661 gcgggttgca ggataaacag catccacggc attacccaca gcagataccc ccagaccacc
    21721 gccgtccagc ttccatctag gtttagccat agcgccagcg tgtactggcc cgccaccagt
    21781 ggcagagcgg gtaataaaat gggcaaccat agccagaaat gccgacgtga cgatccccat
    21841 tccagccacg ccagtatcag gattaaagcg ataaacgtcg cggtaagtcc catcgtcagg
    21901 ctattgatga gcgcttcact attgatcgtc gactgatccg cgaggatcgc caacagaacc
    21961 acacacagca caccggtcaa gggtaaaaaa ctcgccagag taatgccagg caataaaggc
    22021 gtggcaggct tacgaacgcc atctacgcgg ggaatagtac gcctccagct acgccatagc
    22081 agatagccca gcaaaacgta ggcggcgagt aacagcatca gcagcaggct ggcgagtgcg
    22141 cctttcgctt gttgatcggc gtcgccctga gttaaccact gccagctaat tactgccagc
    22201 gtcgggggat tacctggccc aaggataatc gccacatcca cgaccgacag cgaccaggcc
    22261 acaatcgcca gcatcgccat tgccagcgca ggcgcgacag agggcaacag cagccagttc
    22321 aggcattgcc agcggctgta gcccagcgaa tccagcacaa tgagttgctg caacagccgt
    22381 ttttcgctca atactgccgc taagatccac agcagaaatg cgctttcttt cactgccagg
    22441 gtgaggccca gcccaatacc caatttgtcc attggcggag tgaaatacgg gaaatagtca
    22501 taaagcagcc ctccgtcagc aaagagcaga agggcgctgg tggcaaaagc cacatgggga
    22561 atggcgagca gccacggcag acgggcgcac atacgctgcc atttcggccc tggccacagc
    22621 gctacaatca ccagcagggc aatcaacaac gccccgaccg ccgcaatggt tgtcgacacc
    22681 agcgttgcca gtaatgcctg cggtaactgt ggatcggcaa ataacgcctg ccagtgcgtc
    22741 aacgacaaag caggcgatat cagcgtgaga actgccggga tcagcggtgc ataaatcacc
    22801 gccatcatcg cccacagcaa aaagattaat gcgtaccgta acggtgtagc cattcttgtt
    22861 ccagcgcatt tacccaacct gcgtgcggtt cagccagtac cggcggcaga tcctgcggca
    22921 ttctcgattg caatgattcg cgctgcccgt caggcagttt ttgcggatcg agaacagaag
    22981 gatcgcccca gacagcggga tctgctttac gcagttgtgc atcgggtgag agcaggaaat
    23041 tggcaactac cttcgccgca gcactggcat tcgcgttggc gggaatggtg acgaaatgga
    23101 cgttgccaat catcccctcg ctaaagccaa agctgtaact gctggcgggt aaatcgccgc
    23161 tggcgatttt ttgctgcgca tgagcaggat taaaggtcag cgacaggcgc aatgtgttag
    23221 ctttcagcag agcatccatc cgcgcgggcg aaggcgggaa atcctttcct tcgcgccata
    23281 aatacggatg cagcgcatca agaaattgcc acaagggcgc agtgacacgg gcgaaagtcg
    23341 catcgtccgg cgcttctttt aatgcggcgg gatcgggtgt caacataatc agcaactgtt
    23401 caagaaacgc cgtgccggta aagtccggtg ggcgcggata ggtaaccgtg ccgggattgg
    23461 ctttagcaaa ctccagtaag gcttgcggcg tttgtggtgg ctgtggcgta acatcgcggc
    23521 gggcgataaa cgtcagttgt gcgccgcccc agggcgattc agccccttct gtaggcactg
    23581 aaaaatcttc ccgcaccgga agctgtgtgt cgacatagcg ccagttgggc agagtctctg
    23641 cccagtcggt ttgcagtaaa ttagcctctt ttaaggtgcg gaagttttcg ccgttcaccc
    23701 agagcagatc caccgaaccg cctgttttgc gtccggcagc agcttcggtc tgaatgcgct
    23761 tcaccgcgtc tgcggcatcc gcgagatgaa caatcttcag gtttatatcg taatgggttt
    23821 tcatctcgcc gctaacccag tcgagatagc ggttaattgc ggtatcgccg ccccaggcgt
    23881 taaaccagac ggtttgccct ttggcgtcgt ttttgatttc ctgccagtca ctggcatgtg
    23941 ttgccaaaga aaacagcgat aacaatccca gcaaccacac acaatggcgc atcatctctc
    24001 cttaagaacg tttttcagga atacggcaca gcccctgagc gggaatgtcc atggcagaat
    24061 taaaacgggc tgacaggttt tgcagtccga tcaatgccgt cagttcggtg agcgcctgag
    24121 cgtcaaaatg cgtaaccagt cgggcgcgca gggcatcatc gaccgttggc ggcgttacgc
    24181 ttgcggcttc tgcgtactcc agcgccagcc gttcttcatc gctaaagagc gggctttgcc
    24241 gccagtcagc cactgccagc agtttatcgg tgctgccagt gcgctcggcg actttcatac
    24301 tggtgatatc cacgcaaaac tcacacaggc acatttgcgc aatgcgcgcg ctgacaagcg
    24361 atcgcactac cggatccagc ggtgagcgtt tgcgctccag ccagccgaca aatatcgaca
    24421 ccagataaaa gataaacggg atccgccccc accagcgaat cgggcttagc acctcgccgt
    24481 aatggcgacg atgcagccac gcctgcggac gtaaaatgaa aggaattttg ctaagcggcg
    24541 gtaaacccat caggcattcc tcttctggcg ctcaagccat attttcttcg ccataaaaat
    24601 caccacggtc agggcaaaca gaataaacag tccggtgaca aaccagaaag taccgcctgt
    24661 taacatgctg cccgcccagg aataaacaat agtcgctggt aactgaccaa gcccggtggc
    24721 gataaaaaac gagcgaaaac gtattgaagt caaaccggca gcatagctta cgggatcaaa
    24781 aggcacaaaa ggcaataatc ggcataccag aatggtgtgt ttaccatagc gggtaaaaaa
    24841 gccgtccata ctattgagta ccgttttacc cgttaacttt tccaccactt cgcggcccat
    24901 cactctggcg ataaaaaagc acagcgccgc gccggccatc gaactggtcc acgacagcaa
    24961 gccgccccag aacgcgccaa acagcgacgc attggcaaag gtgatcaaaa acgcaggtag
    25021 cggtgcagca atggcctgta aaatcatcaa caagaacgag acaactgccg ccagtgcgcc
    25081 gtaagactga ataaagcgtt ctataccctg ttggtccacg gcggcaaacg ccgcaacgct
    25141 gcgattgata aactcatgga cgccgggaag cagcgcccat gccagcagca tagcgaacaa
    25201 caggatgatg agggttatac ggtagtacca gatttttcgc gattgcatct tcatcatcct
    25261 ttatcctgct ttattcggtg attttaaagg taaccgttgc cagcgtgtta tccgccggga
    25321 gaaccgaggc attgcctttg aatttaactt cacctcgttt ttcaaccgca ccataagtgt
    25381 atgttgcatt gctgacgatg ccgaccgggc agctatccag acacaccagg cagcccgttt
    25441 tcttctcttc tgctgccgtt aaattaccgc caaagcgcat gtccagtttc tttccattac
    25501 tgtcaacaat cacttcatcg aaggaatacg cttttgccgc cccttgccag ttgaccgaaa
    25561 tatccagttt gctgcctgtg acatgagtcg tttctttatt atccatcgtc atgttttcgc
    25621 ccggtgtacc acctgcctct ttcagggctt cataaaaggc tttcggggtc gcataaccca
    25681 taaacagcga tttatgtccg ttggagccat ctttaaacac aataccgtga cgagtgtcgt
    25741 cggtgaggaa acgaccatta atttgcacca gcatggttac cgtatggtta ttggcatcga
    25801 cacgcagcgg ttggctgttc gaaagtccgt cgtattccac ttttgcagcc gcattctctt
    25861 tttgatcgca gccgctaagc ccggcaacag ccagtaagca gagtgcagcc agtccttttt
    25921 tccatgacac tgaataatgt tgcaacatcg ttctatcctt catttttggg gtgagaaagt
    25981 gggctgcggc gcgaagcaga cagatccaca tgtttgtgtc gggcgtagag ttttgcgagc
    26041 tggacgagaa taaacagtgc caggcccgcc agacagagtt gtaaaataaa gcgcagcgta
    26101 atgccttcat tggcgagatc gcttgccatc acggtataaa taacaatacc gggcagggtt
    26161 gtgagtgccg aaataagggt ataaggccag aaggcgattg tggttaatcc gtaagcgtaa
    26221 ttttgaatat tgtaaggaaa caacgggatt aagcgggtca gaataagaaa atcaataccg
    26281 ttacgcgcaa tgcctttttc aatggcctga aaggtatggc tatggccaac gtatttcagc
    26341 agtaaatcac gtcccagcca gcgcgccagc aggaatgagc acgaggaggc cagcgtggcg
    26401 gcaattaatg atagtagtgt ccctaagagc gggccaaaaa ctattcctcc agcgatcacc
    26461 agtatgcttc ctggtaacag aaagagggtg gcaatgatga ataacagaat atagaggcta
    26521 taaccgaaca atccgctctg gcgaatgagt gtcagtaagt gaggtaaatc ggtgagcagc
    26581 tcgaataaac cgaaagcgtg gaaagcgtaa atgaccagcg caaaaataag acaggcaaaa
    26641 agaaatttac gctcagcgtt catcgtggag tttcctatat caaacttgat aatagagaag
    26701 tggtcagaca actaaaaacg cagtgacggt ataacaaagg acggcaggca acaaatcatt
    26761 actgacagtt tgttgatttt tctcaaggtt aattctcctg acccagtttg agccaggaga
    26821 acagctaaat tagcggcgga aggtcgccca gacgggggcg tgatcggacg gtttttccat
    26881 gctgcggatt tcatagtcga tgccggtttc tacgcaacat tctgccagcg gttggctggc
    26941 gagaagcagg tcgatgcgca gaccacggtt atcgtcaaaa ccttttgagc ggtaatcaaa
    27001 ccatgagaaa cgattggctg tttgcggatt cgcatggcgg aaggtatcaa ccaatcccca
    27061 gctcatcagt ctctccatcc attcgcgttc ctccggaagg aaagaacatt ttccggtacg
    27121 cagccagcgc ttacggttct cttcgccaat gccaatatcc aggtcgccag gactgatatt
    27181 catatcgccc ataatcagca ccggattttc acgcttgagt tcggtttcca gatagttttg
    27241 cagattctga taaaactgcg cctttgccgg gaattttatc ggatggtcgc ggctttcacc
    27301 ctgcgggaag taaccgttga tcacggtgac attgcccagc ggcgagggga tttccgccat
    27361 aataatccgc cgttgcgcct cttcgtcgtc accgggaaag ccgcgacgca cggcaatcgg
    27421 cgtctctttg gtcagcagcg ccacgccata atggcctttc tgtccgtgat aaaacacgtt
    27481 gtagccgagc ttcgccacct cttcgagcgg aaacatatcg tcatgaactt ttgtctcctg
    27541 caggccaatc acatccggtt ggtgcttttc gacgatggct tcaagctggt gaggtctggc
    27601 gcgcaggccg ttgatattaa aagagacaaa tttcatagtc gctgccattt attcagataa
    27661 agagtgcgtg gatggtagca gaatttcgct ttgcgtaccg cctgttgtta agtgatttga
    27721 tcgatggttg ttaatgatgt caacgatggc gcaaaaaatg cccgcttttg gtgcgcgttg
    27781 cgtcagaatg gcgcagtaat ttccagtaaa tttcgtcaat gatcacaatt ccagaattat
    27841 attttacttc tgcat
//
LOCUS       DAZUCC010000043        27836 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZUCC010000043 DAZUCC010000000
VERSION     DAZUCC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.043"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            96..338
                     /gene="yjbE"
                     /locus_tag="ACHY09_001643"
     CDS             96..338
                     /gene="yjbE"
                     /locus_tag="ACHY09_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFS3884628.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAASGGGDGSNTGTTTTTTTSTQ"
     gene            525..1163
                     /gene="yjbF"
                     /locus_tag="ACHY09_001644"
     CDS             525..1163
                     /gene="yjbF"
                     /locus_tag="ACHY09_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFS3884629.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWIEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1160..1897
                     /gene="yjbG"
                     /locus_tag="ACHY09_001645"
     CDS             1160..1897
                     /gene="yjbG"
                     /locus_tag="ACHY09_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFS3884630.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLARLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1897..3993
                     /gene="yjbH"
                     /locus_tag="ACHY09_001646"
     CDS             1897..3993
                     /gene="yjbH"
                     /locus_tag="ACHY09_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFS3884631.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQTKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4040..4318)
                     /locus_tag="ACHY09_001647"
     CDS             complement(4040..4318)
                     /locus_tag="ACHY09_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884632.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4532..4942
                     /gene="psiE"
                     /locus_tag="ACHY09_001648"
     CDS             4532..4942
                     /gene="psiE"
                     /locus_tag="ACHY09_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFS3884633.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5036..5926)
                     /gene="malG"
                     /locus_tag="ACHY09_001649"
     CDS             complement(5036..5926)
                     /gene="malG"
                     /locus_tag="ACHY09_001649"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFS3884634.1"
                     /translation="MAMVQPKSQKTRLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRVTPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5941..7485)
                     /gene="malF"
                     /locus_tag="ACHY09_001650"
     CDS             complement(5941..7485)
                     /gene="malF"
                     /locus_tag="ACHY09_001650"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFS3884635.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7639..8829)
                     /gene="malE"
                     /locus_tag="ACHY09_001651"
     CDS             complement(7639..8829)
                     /gene="malE"
                     /locus_tag="ACHY09_001651"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFS3884636.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            9194..10309
                     /gene="malK"
                     /locus_tag="ACHY09_001652"
     CDS             9194..10309
                     /gene="malK"
                     /locus_tag="ACHY09_001652"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFS3884637.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10381..11721
                     /gene="lamB"
                     /locus_tag="ACHY09_001653"
     CDS             10381..11721
                     /gene="lamB"
                     /locus_tag="ACHY09_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFS3884638.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11959..12879
                     /gene="malM"
                     /locus_tag="ACHY09_001654"
     CDS             11959..12879
                     /gene="malM"
                     /locus_tag="ACHY09_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFS3884639.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGIRGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            13058..13555
                     /gene="ubiC"
                     /locus_tag="ACHY09_001655"
     CDS             13058..13555
                     /gene="ubiC"
                     /locus_tag="ACHY09_001655"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFS3884640.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13568..14440
                     /gene="ubiA"
                     /locus_tag="ACHY09_001656"
     CDS             13568..14440
                     /gene="ubiA"
                     /locus_tag="ACHY09_001656"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFS3884641.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14595..17018)
                     /gene="plsB"
                     /locus_tag="ACHY09_001657"
     CDS             complement(14595..17018)
                     /gene="plsB"
                     /locus_tag="ACHY09_001657"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFS3884642.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPGVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17189..17557
                     /gene="dgkA"
                     /locus_tag="ACHY09_001658"
     CDS             17189..17557
                     /gene="dgkA"
                     /locus_tag="ACHY09_001658"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS3884643.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17667..18275
                     /gene="lexA"
                     /locus_tag="ACHY09_001659"
     CDS             17667..18275
                     /gene="lexA"
                     /locus_tag="ACHY09_001659"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS3884644.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18348..19673
                     /gene="dinF"
                     /locus_tag="ACHY09_001660"
     CDS             18348..19673
                     /gene="dinF"
                     /locus_tag="ACHY09_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFS3884645.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            19789..19998
                     /gene="yjbJ"
                     /locus_tag="ACHY09_001661"
     CDS             19789..19998
                     /gene="yjbJ"
                     /locus_tag="ACHY09_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS3884646.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(20040..20555)
                     /gene="zur"
                     /locus_tag="ACHY09_001662"
     CDS             complement(20040..20555)
                     /gene="zur"
                     /locus_tag="ACHY09_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFS3884647.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20711..21706
                     /gene="dusA"
                     /locus_tag="ACHY09_001663"
     CDS             20711..21706
                     /gene="dusA"
                     /locus_tag="ACHY09_001663"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFS3884648.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDVDPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21840..22082
                     /gene="pspG"
                     /locus_tag="ACHY09_001664"
     CDS             21840..22082
                     /gene="pspG"
                     /locus_tag="ACHY09_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFS3884649.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(22248..23231)
                     /gene="qorA"
                     /locus_tag="ACHY09_001665"
     CDS             complement(22248..23231)
                     /gene="qorA"
                     /locus_tag="ACHY09_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFS3884650.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            23314..24729
                     /gene="dnaB"
                     /locus_tag="ACHY09_001666"
     CDS             23314..24729
                     /gene="dnaB"
                     /locus_tag="ACHY09_001666"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS3884651.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            24782..25861
                     /gene="alr"
                     /locus_tag="ACHY09_001667"
     CDS             24782..25861
                     /gene="alr"
                     /locus_tag="ACHY09_001667"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS3884652.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            26114..27307
                     /gene="tyrB"
                     /locus_tag="ACHY09_001668"
     CDS             26114..27307
                     /gene="tyrB"
                     /locus_tag="ACHY09_001668"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFS3884653.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(27529..>27836)
                     /locus_tag="ACHY09_001669"
     CDS             complement(27529..>27836)
                     /locus_tag="ACHY09_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884654.1"
                     /translation="VFHAFIDLVNKIRGKKDCMYICTLCCFAEEVKGDYSHYRTFLFD
                     IGNQYKVKLTQSGKKEFSLTLEFNDTIIESQIVTGNKAKHILEDIEKFYRNKPDTYY"
BASE COUNT         6524 a         7049 c         7499 g         6764 t
ORIGIN      
        1 ccgcgccgta attcgcatgc tttagttgtg tatactcgat cccgcccgaa atgtttttgg
       61 gtaaatctcc attcattcaa tgaagggaaa ttgttatgaa aaaagttctg tatggcattt
      121 ttgccatatc tgcgcttgcg gcgacttctg cgtgggctgc acctgtacag gtgggcgaag
      181 cggcagggtc ggcagcaacg tcggtttcgg cggggagttc ctccgcgacc agcgtcagca
      241 ccgtaagctc ggcggtgggt gtcgcgctcg cggcaagcgg tggcggtgat ggttctaata
      301 ccgggaccac aacaaccacg accaccagta cccagtaata aagtatgtat ccccaaaata
      361 attcgagttg caggaaggcg acaagcgagt gagtccccag gagcatagat aactatgtga
      421 ccggggtgaa cgagtgcggt caccgcatct gcagcttgaa gtatgaaggg gattaaccat
      481 aaccacactc cggtgtggtt attctgcccc tctggagaag agtcgtgaag cgacctgcac
      541 tcattcttat ctgcctgcta ttacaggcct gttcagccac gactaaagag ctgggcaatt
      601 cactgtggga cagtctgttc ggcacgccag gcgtacagct gacggacgat gatattcaaa
      661 atatgcccta cgccagccag tacatgcagc ttaatggcgg gccgcagtta tttgtggtgc
      721 tggccttcgc tgaagacgga caacaaaaat gggtcactca ggatcaggcc actctcgtca
      781 cacaacatgg tcgtctggtg aagactttgc ttggcggcga caacctgatt gaagtgaata
      841 accttgccgc cgacccgctg attaaacccg cacaaattgt tgatggtgca acatggacgc
      901 gcacgatggg ctggatcgag taccagcagg tacgctacgc caccgcacgc tcagtcttca
      961 aatgggatgg caccgatacc gtcaaggtcg gcagcgatga aaccccggtt cgcgtgctgg
     1021 acgaagaagt ctccaccgac caggcgcgct ggcataaccg ctactggatc gacagcgaag
     1081 ggcaaattcg ccagtcggaa cagtatctcg gcgcggatta tttcccggtg aaaaccactc
     1141 tcatcaaggc ggcaaaacaa tgattaaaca aactattgtc gcattgattt tgagtgtggg
     1201 ggcgtcatcg gtcttcgcgg caggaaccgt caaggtgttc agcaatggca gcagtgaggc
     1261 caaaacgcta acgggcgcag agcatttaat cgatctggta gggcaacctc gactggcaaa
     1321 cagctggtgg cctggtgcag tgatcagcga agagctggca acggcggcag cattgcggca
     1381 gcagcaggcg ctactggcac ggctggcaga actggccgca gattccagcg ctgacgatgc
     1441 cgctgcgatt aacgccttac gccagcaaat tcaggcgttg aaagtgacgg gcaggcaaaa
     1501 aatcaatctt gatcccgata tcgtgcgcgt tgccgaacgt ggtaacccac cgttgcaggg
     1561 caactacaca ctgtgggtcg gaccaccacc gtccactgtc acgttgttcg ggcttatcag
     1621 ccgtcctggc aagcagccat tcactccagg tcgcgacgtg gcgagctatc tctccgggca
     1681 gaacctgctt agcggtgcgg atcgcagcta cgcgtgggtg gtttacccgg acggacgcac
     1741 gcaaaaagcg ccggtggctt actggaacaa gcgtcacgta gagccgatgc ccggcagcat
     1801 tatttatgtt ggcctcgcgg actccgtctg gagtgagaca cctgatgccc ttaacgccga
     1861 cattcttcag actctgacgc agcggatacc ccaataatga aaaaaagaca tctgcttagc
     1921 ttactggcgc tgggtattag cacagcttgc tacggcgaaa catatcctgc gcccattggt
     1981 ccgtcgcaat cggatttcgg tggcgtagga ttattacaaa cgcccaccgc gcgcatggcg
     2041 cgggaagggg agctgagtct gaactatcgc gataacgatc agtaccgtta ttactcagct
     2101 tcagtgcaac tcttcccgtg gctggaaaca acgctgcgct acaccgacgt gcgcacccgg
     2161 cagtacagca gcgtcgaagc gttctctggc gatcaaacgt ataaagataa agccttcgat
     2221 ctcaaactgc gtttgtggga agagagttac tggctgccgc aagtggcggt tggtgcgcgg
     2281 gatatcggcg gtacggggct gttcgatgcg gaatatcttg tcgccagcaa agcctggggg
     2341 ccattcgatt ttacgctcgg cctgggctgg ggatatctgg gcaccagcgg taatgtgaaa
     2401 aatccgctct gttcggccag tgacaaatat tgctatcgcg ataacagcta caaacaagcg
     2461 ggatctatcg acggcagcca gatgttccac ggtccggcct cactgtttgg cggcgtggaa
     2521 taccagacgc cctggcaacc gctgcgcctg aaactggagt atgaaggcaa taattatcag
     2581 caggattttg ccggtaagct ggagcaaaaa agtaagttta acgtcggggc gatttatcgc
     2641 gttaccgatt gggccgacgt taaccttagc tatgaacgtg gcaacacttt tatgtttggc
     2701 gtcacgctgc gcaccaactt taacgatctg cgcccgtcct acaacgataa cgcccgcccg
     2761 caatatcaac cgcaaccaca ggatgccatt ttgcagcatt cggtggtggc gaatcagtta
     2821 acgctgttga aatacaacgc cggactggcc gatccgcaga tccagacgaa aggcgatacg
     2881 ctgtatgtca ccggcgagca ggtgaaatat cgtgattcgc gtgaagggat catccgtgct
     2941 aatcggatcg tgatgaacga tctgccggat gggatcaaaa cgatccgcat tacggaaaat
     3001 cgccttaaca tgccgcaggt gacgacggaa accgatgtcg ccagcctgaa aaatcatctc
     3061 gccggagagc cgttgggcca cgaaacgaag ctggcgcaaa aacgcgtcga gccagtggtt
     3121 ccgcagtcca ccgagcaggg ctggtatatc gacaaatcac gctttgattt ccatatcgat
     3181 ccggtgttga accagtcggt cggtggcccg gaaaactttt acatgtatca gctgggcgtg
     3241 atgggaacgg cggatttgtg gctgacggac catctgctga ccaccggcag cctgtttgct
     3301 aaccttgcca acaactacga caagtttaac tacaccaatc cgccgcagga ctcgcactta
     3361 ccgcgcgttc gtacccatgt gcgcgagtat gtgcagaacg atgtctatgt gaataacctg
     3421 caagccaact acttccagca tctgggcaac ggcttctacg gtcaggtcta cggaggttat
     3481 ctcgaaacca tgtttggcgg tgcgggggca gaagtgttgt atcgcccgct ggacagcaac
     3541 tgggcgtttg gtctggatgc caactacgtt aaacagcgcg actggcgtag tgcaaaagat
     3601 atgatgaaat tcaccgacta cagcgtgaaa accggacatc tgaccgcata ctggacgcca
     3661 tcttttgctc aggatgtgtt agtgaaagcg agcgtcgggc agtatctggc aggggataaa
     3721 ggcggcacgc tggagatcgc caaacgcttt gatagcggcg tggtggtggg tggctatgcc
     3781 acgatcacca acgtttcgaa agaggagtac ggcgaagggg actttaccaa aggcgtgtat
     3841 gtctctgtac cgctggatct cttctcgtct ggcccgacac gtagccgtgc ggcaattggc
     3901 tggacgccgc tgacgcgtga cggtggtcag caacttgggc gtaagttcca gttgtatgac
     3961 atgaccagcg accgtagcgt caatttccgc taagtcatgg aaaaggtgcc agttttcgca
     4021 ctcactggca ccctcattct taatggcctg tcgagccatc aggaacgcag ttcacccccg
     4081 gtttgcgatc tggtgtgtaa aggcgaaaac attctgtctt cctgtccgtc gatatcggtt
     4141 gcaggtctgg ttgcctgagc gactgggcca tcattggact ggagacattg ctttgcggta
     4201 ggtcggcttt atcagcagta ccgagcggac cagcataagc aggaagaaca gagactgata
     4261 acatcaaagc agcaaaataa ggcttcattt ttaccacctt tatcaggtta cgtttcattt
     4321 gttccagagg aacattgtcg atttttcgcg cattgctggt ggctgggaat cacctgaatg
     4381 ggtgattttt ttggctgtgt gttttgccag atgagacatt gatgggagat gcccacttaa
     4441 ttgagctgaa caaaacatac acaaaaaata tagatctccg tcacattttt gcgttataca
     4501 ggaagctcgc cactgtgaag gaggtactgc tatgacgtca ctctctcgtc cgcgcgtgga
     4561 gtttatatcc accattttgc agaccgtact caatcttggc ctgttgtgcc tcggcctgat
     4621 tttggttgtc ttcctcggca aagaaacggt gcatctggct gatgtgctgt tcgcgccaga
     4681 acaaaccagt aaatatgagc tggtagaagg gctggtggtt tactttctct atttcgaatt
     4741 tatcgcgctg attgtgaagt actttcagtc cggttttcac ttcccgctac gttactttgt
     4801 ctatattggg atcaccgcca ttgtgcgctt gatcatcgtc gatcataaat cgccactgga
     4861 tgtgttgatc tactcggcag cgatcctgtt actggtgatc accctgtggt tgtgtaattc
     4921 gaaacggctg aagcgggagt aaaaaaaggc gctccgtgga gcgccgaata acagtcacaa
     4981 gttgggataa cgtaagttga gggtgcagcg gcataacatt ggcagaacaa catctttaac
     5041 ctttcacacc acctgccgtc aggccgttga ccagccagcg ttgagccagc aagaagacga
     5101 tggtgatcgg taatgcagac atcacggcag cggcggcaaa gtcaccccac aggtagtttt
     5161 gcgggttgag gtattgctgc atccccacag ccagggtgta gctgtttacg tcacgcagta
     5221 atagcgacgc gaccggaact tcagtaatgg cagcgataaa cgacaggatg aacacaaccg
     5281 ccagaatcgg tactgacagc ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac
     5341 catccagcgc cgccgcttct tccagcgaac tgtcgatggt ttcgaaataa cctttgatgg
     5401 tccagacatg cagcgcaatc ccgcccagat acgcgaaaat tacgccgccg tgagtattca
     5461 ggccaatgaa tggaatgtac tcacccagac gatcaaacaa cgcatacaac gcgaccagtg
     5521 aaagtactgc cgggaacatc tggaaaatca gcattccttt cagcagcgtc gctttgcctg
     5581 gaaagcgcat acgggcgaaa gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg
     5641 cggaaatccc ggcgaccttt accgagttcc acagccatag cagtaccgga aacggcggcg
     5701 gtgtaacgcg accatcagcc tgttccacgc taaaacctaa tgccagtttc cagtgatccc
     5761 aggagatttg ctccgggatc aggctgccgg tcgcaaagtt cccctggcgc agtgagatag
     5821 cgacgaccat cagtagcggg aacataatcg ccgcgataaa aagtagcagt agcaggtgag
     5881 taataaacaa acgtgttttt tgcgatttcg gttggaccat tgccattttt gttatctccc
     5941 ttaatcaaac ttcattcgcg tggctttcag gttcactatc gccagcgcac ccaccagcag
     6001 gaagatcagc gtggcaattg ctgccgccag accgaagtcc tgacccccgc cgccttcaaa
     6061 agcgatgcgg taggtgtagt taacaagcag gtcggtataa ccggctggcg tggtcgtgcc
     6121 aagacgatcc gggccgccgt tggttaacag ttgaatcagc acgaagttgt taaagttaaa
     6181 ggcgaagctg gcgatcatca gcggcgtcag cggtttaatc agcagcggca gcgtaatctt
     6241 aaagaagttc tggaacggac ctgcgccatc cattgctgag gcttcataca aatcgtccgg
     6301 aatcgctttc agcaagccca tgcagaggat catcatgtac ggataaccca gccaggtatt
     6361 gacgataatc agcatcgttc gggcggtggt cggatcgctg aaccaggcgg gcttcacgcc
     6421 aaacagcgcg ctcaacatca tgttgatctc accgaagctc tggttaaaca accctttgaa
     6481 aatcaagatt gaaatgaacg atggcaccgc gtagggcaga atcagcagga cgcgatagac
     6541 cgctttgccg cgcaacgctt cccactgcac cagacacgcc agaaccatgc cgaccgccac
     6601 cgttaaaaag acagtgatca gcgagaacac cacggtccag acgaaaatgg cgaggaacgg
     6661 tttctgaatg ccttcgtcgg tgaagacgcg ggtaaagttt ttccagccag tggtcacggt
     6721 gtaaccgggg cttagctttt catcgcccca gttgccgtcg gcggtaatgg actggtaaaa
     6781 gccaatttgg ttattcggac gatatttcac gccgctctga ttattcgtca acgtgccgtc
     6841 accgtcgagt gtgtagagcg gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac
     6901 tttgttgcca tccggcagaa tggcggtaat gtcactcagc gcctgacgat tctgggtaat
     6961 cacgcgtaga ttcgcgcgtt cgccttcggg ctgggcggtc gtttctttca gttgcagttt
     7021 ttgctcgccg ccaaatttaa aagcgtcgga aaggtaattt ttgccggttt cgccgtcgct
     7081 gagcgccagt tgccactcat cgcccgccgg gtaaagacca aagttatagg ttttgcctgc
     7141 ttgccaggag cgatctaaca acacttcctg tgcacgttca aaagtcagct ggttagtgct
     7201 gctgtagttg gtgaaggcaa tggcgatggt gcagaccaga gggaagagga cgaataatcc
     7261 cattccagcc attcccgggt aaacatagcg ccaggcgtag gctttacgat tggcgaaaat
     7321 atacagcccc gctgaactca atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc
     7381 gtacattaaa acaacaaggt aacccaccag caggccgagc agacctaaca ctgaccattt
     7441 cagcgcgtcg ctttgccacc aatgtttctt tttaatgaca tccatggggt tcttcctcat
     7501 tccaggacgg ataaggcttt cacgccttat ccgacaacaa ctgcctgatg cgacgctgac
     7561 gcgtcttatc aggcctacat acgtttcaat ttcgtaggcc ggataaggcg tttacgccgc
     7621 atccggcatt tcacagcatt acttggtgat acgagtctgc gcgtctttca gggcttcatc
     7681 gacagtctga cgaccgctgg cggcgttgat caccgcagta cgcacggcat accagaaagc
     7741 ggacatctgc gggatgttcg gcataatttc acctttctga gcgttatcca tggtggcggc
     7801 aatacgtgga tctttcgcaa gttcatcctg ataggacttc agcgctactg cacccagcgg
     7861 tttgtcttta ttgaccgctt ccagaccttc gtcagtcagc agatagtttt cgaggaactc
     7921 tttcgccagc tctttgttcg gactggcggc gttaatacct gcgctcagca cgccaacgaa
     7981 cggtttagat ggttgacctt tgaaggtcgg cagtaccgtt acaccataat tcactttact
     8041 ggtgtcgatg ttggaccatg cccacgggcc gttgatagtc atcgctgttt cgcctttatt
     8101 aaaggcagct tctgcgatgg agtaatcggt gtctgcattc atgtgtttgt ttttaatcag
     8161 atcaaccagg aaggttagac ccgctttcgc gccagcgtta tccacgccta cgtctttaat
     8221 gtcgtacttg ccgttttcat acttgaacgc ataacccccg tcagccgcaa ttagcggcca
     8281 ggtgaagtat ggttcttgca ggttgaacat cagcgcgctc ttacctttcg ctttcagttc
     8341 tttatccagc gccgggatct cttcccaggt ttttggcggg ttcggtagca gatctttgtt
     8401 ataaatcagt gataacgctt cgacagcgat cgggtaagca atcagcttgc cgttgtaacg
     8461 tacggcatcc caggtaaacg gatacagctt gtcctggaac gctttgtccg gggtgatttc
     8521 agccaacagg ccagattgag cgtagccgcc aaagcggtcg tgcgcccaga agataatgtc
     8581 agggccatcg ccagttgccg caacctgagg gaatttctct tccagtttat ccggatgctc
     8641 aacggtgact ttaattccgg tatctttctc gaatttctta ccgacttcag cgagaccgtt
     8701 atagccttta tcgccgttaa tccagattac cagtttacct tcttcgattt tggcgagagc
     8761 cgaggcggaa aacatcatcg tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt
     8821 tattttcata atctatggtc cttgttggtg aagtgctcgt gaaaacacct aaacggactc
     8881 tagtttcttt atacggcaac ctctttccat cctccttgcc cctacgcccc accgttgctt
     8941 tgtgtgatct ctgttacaga attggcggta atgtggagat gcgcacataa aatcgccatg
     9001 atttttgcaa gcaacatcac gaaattcctt acatgacctc ggtttagttc acagaagctg
     9061 cgttctcatc ctcccgcctc ctcccccata aaaaagccag ggggtggagg atttaagcca
     9121 tctcctgatg acgcatagtc agcccatcat gaatgttgct gtcgatgaca ggttgttaca
     9181 aagggagaag ggcatggcga gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt
     9241 cgtggtatcg aaagatatca atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg
     9301 accgtctggc tgcggtaaat cgactttact gcgcatgatt gccgggcttg agacgatcac
     9361 cagcggcgac ctgttcatcg gtgagaaacg gatgaatgac actccgcctg cagaacgcgg
     9421 cgttggtatg gtgtttcagt cttacgcgct ctatccccac ctgtcagtag cagaaaacat
     9481 gtcatttggc ctgaaactgg ctggcgcgaa aaaagaagtg attaaccagc gagttaacca
     9541 ggttgcggaa gtgctgcaac tggcgcattt gctggatcgc aaaccgaaag cgctctccgg
     9601 tggtcagcgt cagcgtgtgg cgattggccg tacgctggta gccgagccaa gcgtattttt
     9661 gctcgatgaa ccgctctcca acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat
     9721 ctcccgtctg cataaacgcc tgggccgcac aatgatttac gtcacccacg atcaggtcga
     9781 agcgatgaca ctagccgaca aaatcgtggt gctggacgcc ggtcgcgtgg cgcaggttgg
     9841 gaaaccgctg gagctgtacc actatccggc agaccgtttt gtcgccggat ttatcggttc
     9901 gccaaagatg aacttcctgc cagtaaaagt gaccgccact gcaatcgatc aagtgcaggt
     9961 ggagctgccg atgccaaatc gtcagcaagt ctggctgccg gttgaaagcc gtgatgtcca
    10021 ggttggagcc aatatgtcgc tgggtattcg cccggaacat ctactgccga gtgatatcgc
    10081 tgacgtcatc cttgagggtg aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat
    10141 ccatatccag atcccttcca ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt
    10201 ggtagaagaa ggtgccacat tcgctatcgg cctgccgcca gagcgttgcc atctgttccg
    10261 tgaggatggc actgcatgtc gtcgactgca taaggagccg ggcgtttaag caccccacaa
    10321 aacacacaaa gcctgtcaca ggtgatgtga aaaaagaaaa gcaatgactc aggagataga
    10381 atgatgatta ctctgcgcaa acttcctctg gcggttgccg tcgcagcggg cgtaatgtct
    10441 gctcaggcaa tggctgttga tttccacggc tatgcacgtt ccggtattgg ctggacaggt
    10501 agcggcggtg aacaacagtg tttccagact accggtgctc aaagtaaata ccgtcttggc
    10561 aacgaatgtg aaacttatgc tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat
    10621 aagagcttct atttcgacac taacgtggcc tattccgtcg cacaacagaa tgactgggaa
    10681 gctaccgatc cggccttccg tgaagcaaac gtgcagggta aaaacctgat cgaatggctg
    10741 ccaggttcca ccatctgggc aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc
    10801 gacttctact actgggatat ttctggtcct ggtgccggtc tggaaaacat cgatgttggc
    10861 ttcggtaaac tctctctggc agcaacccgt tcctctgaag ctggtggttc ttcctctttc
    10921 gccagcaaca atatttatga ctataccaac gaaaccgcga acgacgtttt cgacgtgcgt
    10981 ttagcgcaga tggaaatcaa cccgggcggc acattagaac tgggtgtcga ctacggtcgt
    11041 gccaacctgc gtgataacta tcgtctggtt gatggcgcat cgaaagacgg ttggttattc
    11101 actgctgaac atactcagag tgtcctgaag ggctttaaca agtttgttgt tcagtacgct
    11161 actgactcga tgacctcgca gggtaaaggt ctgtcgcagg gttctggcgt cgcgtttgat
    11221 aacgaaaaat ttgcctacaa tatcaacaac aacggtcaca tgctgcgtat cctcgaccac
    11281 ggtgcgatct ccatgggcga caactgggac atgatgtacg tgggtatgta ccaggatatc
    11341 aactgggata acgacaacgg caccaagtgg tggaccgtcg gtattcgccc gatgtacaag
    11401 tggacgccaa tcatgagcac cgtgatggaa atcggctacg acaacgtcga atcccagcgc
    11461 accggcgaca agaacaatca gtacaaaatt acccttgcac aacaatggca ggctggcgac
    11521 agcatctggt cacgcccggc tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa
    11581 tggggttatg actacaccgg taacgccaat accaacacta acttcggcaa agccgttcct
    11641 gctgatttca acggcggcag cttcggccgt ggtgacagcg acgagtggac cttcggtgcc
    11701 cagatggaaa tctggtggta atagcaaaac ctgggccgga taaggtgctt gcgccgcatc
    11761 cggcaaccaa cgcctgatgc gacgcttgcg cgtcttatca ggcctacaac tacaaccgct
    11821 gccgaaagta ggccggataa ggcgtttacg ccgcatccgg cataaaaaca gctttaaggg
    11881 gcgaaagccc ctctgattat cgggtttagc gcgctattgc ctggctaccg ctgagctcca
    11941 gattttgagg tgaaaacaat gaaaatgaat aaaagtctca tcgctctctg tttatcagca
    12001 gggttgctgg caagcgcacc tggaatcagc cttgccgatg ttaactacgt accgcaaaac
    12061 accagcgacg cgccagccat tccatctgcc gcgctgcaac aactcacctg gacaccggtc
    12121 gatcaatcta aaacccagac cacccaactg gcgactggcg gccaacaact gaacgttccc
    12181 ggcatcagag gtccggttgc tgcgtacagc gtcccggcaa acattggcga actgacccta
    12241 acgctgacca gcgaagtgaa caaacaaact agcgtatttg cgccgaacgt gctgattctt
    12301 gatcagaaca tgaccccatc agccttcttc cccagcagtt atttcaccta ccaggaacca
    12361 ggcgtgatga gtgcagatcg tctggaaggc gttatgcgcc tgacaccggc gttggggcag
    12421 caaaaacttt atgttctggt ctttaccacg gaaaaagacc tccagcagac gacccaactg
    12481 ctcgacccgg ctaaagccta tgccaaaggc gtcggtaact cgatcccgga tattcctgac
    12541 ccggttgctc gtcataccac cgatggctta ctgaaactga aagtcaaaac gaactccagt
    12601 tctagcgtgt tggtcgggcc tctatttggt tcttccgctc cagctccggt tacggtaggc
    12661 aacacggcgg caccagctgt ggctgcaccc gctccggcac cggtgaagaa aagcgagccg
    12721 atgctcaacg acacggaaag ttattttaat accgcgatca aaaacgctgt cgcgaaaggt
    12781 gatgttgata aggcgttaaa actgcttgat gaagctgaac gcctggggtc gacatctgcc
    12841 cgttccacct ttatcagcag tgtaaaaggc aaggggtaat tacgccccac agtgctgatt
    12901 ttgcaacaac tggtgcgtct cctggcgcac ctttttttct cctcctgtac tattgttgcg
    12961 tttttgttgc gcattagttc acttaatttg ctttacatct cccgtaaata tttttctgcg
    13021 atacaatgcc tttacgttat gtaacggaga gttcggcatg tcacaccccg cgttaacgca
    13081 actgcgtgcg ctgcgctatt ttacagagat ccctgccctg gagccgcaac tgctcgactg
    13141 gctgttgctg gaagattcca tgacaaaacg ttttgaacag cagggaaaaa cggtgagcgt
    13201 gacgatgatc cgcgaagggt ttgtcgagca gaatgaaatc cccgaagaac tgccactgct
    13261 gccgaaagag tctcgttact ggttacgtga aattttgtta tgtgccgatg gtgaaccgtg
    13321 gcttgccggt cgtaccgtcg ttcctgtgtc aacgttaagc gggccggagc tggcgttaca
    13381 aaaattaggt aaaacgccgt taggacgcta tctgttcaca tcatcgacat taacccggga
    13441 ctttattgag ataggccgtg atgccggact gtgggggcga cgttcccgcc tgcgattaag
    13501 cggtaaaccg ctgttgctaa cagaactgtt tttaccggcg tcaccgttgt actaagagga
    13561 aaaaaatatg gagtggagtc tgacgcagaa taagctgctg gcgtttcatc gcttaatgcg
    13621 tacggataag ccaattggcg cgttactgct gctctggcca acattatggg cgttgtgggt
    13681 ggcgacaccg ggcgttcccc agctctggat cctggcggtg tttgtcgcgg gcgtctggct
    13741 gatgcgcgct gctggttgcg tggtgaatga ttatgctgac cgcaagtttg atggtcatgt
    13801 taagcgcacg gcgaaccgac cactccccag cggcgcggta acggagaaag aggcgcgcgc
    13861 gctgtttgta gtgctggtac tgatttcatt tttactggtg ctgacgctga atacgatgac
    13921 tattctgttg tcgattgccg cgctggcgct ggcgtgggta tacccgttta tgaagcggta
    13981 tacccatcta ccgcaagtgg tgctgggcgc ggcgtttggc tggtcgattc caatggcttt
    14041 tgctgctgtg agtgagtcgg tgccattgag ttgctggtta atgttcctcg ccaatattct
    14101 ctgggcggtg gcatacgaca cgcagtatgc gatggttgac cgtgatgatg atgtgaagat
    14161 tggcattaaa tccaccgcaa tcctgttcgg ccaatacgat aaattgatta ttggtatttt
    14221 gcagattggc gtactggcgc tgatggcgat tatcggtgag ttaaatggct taggctgggg
    14281 atattactgg tcaattctgg tggctggcgc gctgtttgtt tatcaacaaa aactgattgc
    14341 caaccgcgag cgtgaagcct gctttaaagc atttatgaat aataactatg ttggtctggt
    14401 actgttttta gggctggcaa tgagttactg gcatttctga tgatgtaaaa aagccggatg
    14461 atcatccggc ttccttctgg gttgcctgat gcgctgcgct tctcaggcct acacaacata
    14521 tcgcaattta ttgaatttgc agatcatggt aggccgaata aggcgtttgc gccgcatccg
    14581 gcaattctcc ctgattaccc ttcgccctgc gtcgcactct caatcgtcaa acgcacgtct
    14641 gatgtaatca actccgccag caactgatac accttcatcg tttctgccgg ttcggcgtcg
    14701 ccgctatcgc tgatataccc ttcatcacgc agtgtcagca ccagagaact gaacaccgcc
    14761 ttgtcgaaga actccggcgc gttgatgccg tgcagcacgg agagacgttg cgcgacggtg
    14821 cggctctctt tctccagcgt accgcggttg atcgacgggt tggcgctcaa cagccagaag
    14881 gtgatggcat aacgttgcag cgtttcgcgc gcgcctgcgg ccagcagctg tagcgtgcga
    14941 gaatgcgccg ggttgatatg caactcatca tcttgcaggg taatcagccc ctgacgttgc
    15001 atctcatttg ccagtgcatc aataacgccc ggcaactcgt cgcgatccca gcgcaggaac
    15061 agctccgctt tcagcattgg gtaaagcaca ttgacgtgct ccatcaatac gtcgcgggag
    15121 atgtggcgat gctgggtgac aattgccgcc atcagcgaag gcagcaccaa catatgcgca
    15181 atgttgttgc gatagtaggt catcagcacc gcttgctcgc gcggcagaat gatgatgtcg
    15241 ccgatcgtgt ctttctcgac ttcaaacttg ttcatttgca gcgcgtgatc gataagctcg
    15301 ctggcgctgg ctgaaggaac ggtagagtcc gtggagtaag gcacgttgcg catcagatcc
    15361 aggtagcagt tgagttgctc ggttaactgc tcgcgggtga gtgagcgctg acgtgacgcc
    15421 agaagcgcag tacaacacag gttcatggcg tttgccgcgc ctgcgttgtt aatgcgtacc
    15481 atcagatcgg cagcaatatt attgaccgtc ggcgttaacc atgccggacg caccgcttcg
    15541 atgggatcga tagattcacg ccagtcaggt acatgctggt taaggtaggt catcaacggc
    15601 attggttcac cgaagttgac gtaaccctga ccgagattac gcagcttgct taaaccgcgc
    15661 agcatctgcg gcaggctctc tttctctttc gtcgcaccgc gcagttcttt ggcgtaagta
    15721 cccacttcca tgacgtgctc ataaccgata tagatcggaa tcagcgtaat cggacgcgtg
    15781 ccgccacgca gcatcgcctg aatggtcatc gacagcgtac cggttttcgg atccagcaaa
    15841 cgccctgtac gggaacgacc gccttccacg aaatactcga cggaataacc acggctgaac
    15901 agttcgccga gatactcacg gaaaacggtg gaatagagct tattgccttt aaacgtacgg
    15961 cgaataaaga acgcccccag acggcggaaa atcggcccgg caggccagaa attcaggttg
    16021 atcccggcgg cgatatgcgg cggcaccagc ccctggtgat acagcacgta agaaagcagc
    16081 aggtagtcca tgtggctgcg gtggcaaggc acatacacca gctcatgacc gtcgtgggcc
    16141 agctgacgaa cgcgctcggc gttatggacg ttgatgccct ggtaaaggcg gttccaggtg
    16201 aagcccagaa tacggtcagt caggcggatc atctcgtaag agaaattcgc cgcaatctct
    16261 tccatcagcg caatcgcgtt ctgctgcgct ttttcatggg agattttttt gctgcgcgct
    16321 tcatcttcta ccgctttggc aatggcgcgg gaggcgagca gcttattaaa cagatcctga
    16381 cgagcaggca gacgtgggcc tacggcagcc agacgttgac gggcaaagtg catacgcgcc
    16441 acgcgcgcca gtttctgcgc gatagtttta tccgtgccgt gttcatccgc catacggcgc
    16501 agcgaaactg acggcgagaa acgcacaaaa ctgtcgcgac cgagccacag tacagcgaaa
    16561 aatttctgta cgccgttaag catacgcagc ggcgggttca cttcgccttt ttcacgcccc
    16621 ggcgcgcgac caaacatcac cgatactggc accatctgca catccagatt tgggttgcta
    16681 cggtgcaaat cgagatagtc gtggaacagc ttaatagact cttctttcgg cgtgtaatag
    16741 gtgaacacac gcggcccgcc gtgaatgaac acatagcgcg gcagtagcgt gccgtcgatt
    16801 tccagcggct ctaacgggtc aggcaggtca tgtgccagac actgggcgcg caacgtcagc
    16861 aagtcagctt tcgagttgta cggtaaaacg tacataattg gacgagaggt atccagcccc
    16921 agttccgggg caggatctgc cggaatagac ttgcttttta ccaggatgct taatggtaaa
    16981 ttcagtaatt tgtagtaaat tcgtggccag ccggacataa acgatgtaaa gcctctggtt
    17041 aataatgcaa atgcgcggca aggatagcag aaagtcatgg gaaattctgt ggtatccgct
    17101 catgtttcgc gcggcgttac gcaaacccga atcatcggat ttaacggtac actgatattg
    17161 acgctcataa tgtaaaaagg ttctttcaat ggccaataat accactggat tcacccgaat
    17221 tatcaaagct gctggctatt cctggaaagg tttacgcgct gcatggatca acgaagcggc
    17281 attccgtcag gaaggcgtag cggtattgtt ggcggtggtc atcgcctgct ggctggatgt
    17341 ggacgcaatt acccgcgtgc tgcttatcag ctccgtgatg ctggtgatga ttgtggaaat
    17401 cctcaatagc gccatcgaag cagtggttga ccgaattggc tctgaatacc atgagctttc
    17461 cggacgcgct aaagatatgg gatccgccgc ggttctgatt gcgattatcg tcgccgtgat
    17521 tacctggtgc atcctgttat ggtcgcattt tggataaccc ttccagattt cgataaatct
    17581 ctggtttatt gtgcagttta tggttccaaa atcgcctttt gctgtatata ctcacagcat
    17641 aactgtatat acacccaggg ggcggaatga aagcgttaac ggccaggcaa caagaggtgt
    17701 ttgatctcat ccgtgatcac atcagccaga caggtatgcc gccgacgcgt gcggaaatcg
    17761 cgcagcgttt ggggttccgt tccccaaacg cggctgaaga acatctgaag gcgctggcac
    17821 gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg gattcgtctg ttgcaggaag
    17881 aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg tgaaccgctt ctggcgcaac
    17941 agcatattga aggtcattat caggtcgatc cttccttatt caagccgaat gctgatttcc
    18001 tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat tatggatggc gacttgctgg
    18061 cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt tgtcgcacgt attgatgacg
    18121 aggttaccgt taagcgcctg aaaaaacagg gcaataaagt cgaactgttg ccagaaaata
    18181 gcgagtttaa accaattgtc gttgaccttc gtcagcagag cttcaccatt gaagggctgg
    18241 cggttggagt tattcgcaac ggcgactggc tgtaacatat ctctgagacc gcgatgccgc
    18301 ctggcgtcgc ggtttgtttt tcatctctct tcatcaggct tgtctgcatg gcattcctca
    18361 cttcatctga taaagcactc tggcatctcg ccttacccat gattttctcc aatatcaccg
    18421 ttccgttgct gggactggtc gatacggcgg taattggtca tcttgatagt ccggtttatt
    18481 tgggcggcgt ggcggttggt gcaacggcga ccagctttct ctttatgctg ttgctgtttt
    18541 tacgcatgag caccaccggg ctgactgcgc aggcttatgg tgccaaaaat cctcaggcat
    18601 tagcccgtgc gctggtgcaa ccgttgctgt tggcgttggg ggctggggcg ttaattgcgc
    18661 tgctgcgtac gccgattatc gatctggcgc tgcatattgt tggcggcagc gaagcggtgc
    18721 tagaacaggc gcgacgcttt cttgaaatcc gctggttaag tgcaccggcg tcgctggcga
    18781 atctggtatt acttggttgg ttactcggtg tgcaatatgc ccgtgcgcca gtaattttgt
    18841 tagtggtcgg caatatcctc aacattgtgc tggatgtctg gctggtgatg gggctgcata
    18901 tgaacgtgca gggcgcggcg ctggcgacgg ttattgcgga atatgcaaca ttgctgattg
    18961 gtctgctaat ggtgcgtaaa atcctcaaac tacgcggaat ctccggcgaa atgctgaaaa
    19021 ctgcctggcg aggaaacttc cgtcacttgc tggcgcttaa ccgcgatatc atgctgcgct
    19081 cgctgttgtt gcaactctgt ttcggcgcga tcaccgtact tggcgcgcga ctggggagtg
    19141 acattatcgc tgttaacgcg gttctgatga cgttactcac ctttaccgcc tatgcgctgg
    19201 atggttttgc ctacgcggtt gaagcgcatt ccggtcaggc gtacggtgcg cgcgatggta
    19261 gccagttact ggatgtctgg cgggcagcgt gccgccagtc ggggatcgta gcgttactgt
    19321 tttcggtggt ttatttgctg gcaggggaac acatcattgc gttgctgacg tcgttaaccc
    19381 agattcagca gctggctgac cgctatctta tctggcaggt gattttgccg ttggtcggcg
    19441 tctggtgtta tctgctcgac ggcatgttta taggtgcaac gcgcgccgcc gaaatgcgta
    19501 acagtatggc ggtggccgcc gcaggttttg cgctgacgct ccttacgctg ccgtggctgg
    19561 ggaatcatgg tttgtggctg gcattaaccg tctttctggc tttgcgcggg ctttctctgg
    19621 cggctatctg gcggcgtcac tggcgcaacg gtacctggtt tgccgcaacg tgacggttaa
    19681 aaattctgaa taaataatcc taagccaaat tgctgactac acttaatctc acgttcagaa
    19741 gaaaagtgaa cgtactctca ttcacaacct aacgatgagg tcttgattat gaataaagat
    19801 gaagccggcg gtaactggaa acagtttaaa ggtaaagtga aagagcaatg gggaaaactg
    19861 accgatgatg atatgacgat cattgaaggt aaacgtgatc aactggtcgg taaaatccag
    19921 gaacgttatg gttatcagaa agatcaggca gaaaaagagg ttgatagttg ggaaaaacgc
    19981 catgattacc gctggtaatt aatccctcct gcccgacatg tacaaggatg tacacccttt
    20041 tagcgaggtt tctttttcac ctgaatagag tggtcatgat ggcactgttc gggatgacga
    20101 cacgcttcta cttctacaca tgctgaacat aatccatgtg cttcaataac attatgccgc
    20161 agggcaaaac ccatttttgc cgccagcgta tgcatgatgt cttccacgcc ttcagcacat
    20221 tcttctttca ccgcgccgca gcgatcgcaa ataaacatgg ctgaagtatg ggtgggctga
    20281 tcgaacagat gacagagcac ataactgttg gtggattcca ccttatgcac aaaaccttgt
    20341 tcaagcagaa actccagcgc gcgataaacc gttggcggct tggcttgcgg ttcagcttcg
    20401 cgcagcaaat caagcagatc ataagcgctg atagcgccat cctgcagact catcaggcgc
    20461 aatacttcca ggcgctgtgg ggtcaggcgc acattacgct gtgcgcagat tttttcagcc
    20521 tgcgctaata actcctgcgt tgtggtcttt tccattgggc acctcgacag tggggttaag
    20581 aatgtcctta ctttaccata gttctcgctt aaacgccgag aacagggtgt catggctgtc
    20641 acattgcctg atcgcatatt cgttatgcta tagtagcgcc cccaatttac cagatgctta
    20701 aaaagtcgcc atgccagcag aatcacacac tgtttatccc gcttaccgtt tctcgattgc
    20761 cccgatgctt gactggacgg acagacattg ccgctatttc ttgcgtctgc tttcccgcaa
    20821 tacgttgctg tataccgaaa tggtgactac aggggcgatt attcacggta aaggtgatta
    20881 cctggcgtac agtgaagaag aacatccggt agcgttgcaa ctcgggggta gcgatccggc
    20941 ggcgctggca cagtgtgcga agctggcaga agcgcgcgga tatgatgaga tcaacctgaa
    21001 tgtcggctgc ccgtctgacc gggtgcagaa cggcatgttt ggtgcgtgtc tgatgggtaa
    21061 tgcgcagctg gttgccgact gcgtgaaagc gatgcgcgat gtggtgtcga ttccggtgac
    21121 ggtgaaaacg cgtattggca tcgatgatca ggacagctat gaatttctct gcgatttcat
    21181 caacaccgtt tccggcaaag gtgagtgtga gatgttcatc atccacgcac gtaaagcctg
    21241 gctttcgggg ttaagcccga aagaaaaccg tgaaatcccg ccgctcgatt atccgcgtgt
    21301 gtatcaactg aagcgtgact ttccgcatct gacgatgtcg attaacggtg gcatcaagtc
    21361 gctggaagag gctaaagcgc atttgcaaca tatggatggc gtgatggtcg ggcgcgaagc
    21421 gtatcagaat ccaggcattc tggcggcggt agaccgagag atctttggtt cttcggatac
    21481 cgatgtcgat ccggtggcgg tggtgcgtgc catgtatccg tacattgagc gtgaactcag
    21541 ccaggggacg tatctcggcc atattacccg gcatatgctg ggcttgttcc agggtattcc
    21601 tggcgcgcgg cagtggcggc gttatttaag tgaaaatgcc cataaagcgg gtgctgacat
    21661 taacgtgctg gaacacgcgc tcaaactggt ggcggataag cgttaacttt tcaccaaaaa
    21721 gtagtcaaat tcaccacgcc ctgcgcaccg tcgcggggcg ttttgctgtt aaatcaatag
    21781 attatttttg gcatgattct tgtaatgcct gcaagagatt tcatatttgg gagagcatca
    21841 tgctggaact actttttgtg attggctttt ttgtcatgct gatggtcacc ggcgtttcgt
    21901 tgctgggcat tatcgccgcg ctggttgtgg cgacggccat tatgttcctc ggcggtatgc
    21961 tggcattgat gattaagttg ctgccgtggt tactcctggc gattgcggtg gtgtgggtta
    22021 ttaaggcgat taaagcacca aaagtgccga aatatcagcg ttatgaccgc tggcgttact
    22081 aagggattgt gcggatgatc acaacctaag gttttatcct tagaacaaaa taggaattga
    22141 taatcaaatc tgtcactatt gcgcctctaa cagattcatc gtgctgtacc ctacatacag
    22201 ccgaactata aaaagaaagg gcttcccaga tggaagccct atttctttta tggaatcaac
    22261 aggctggaac cttgcgtcgc ccggctttcc agaatctcat gcgcacgctg tgcatcctta
    22321 agcggatatt tctgctgctc ggcgacatcg accttaatca caccgctggc aatcaaagag
    22381 aacagttcat tactggcctc ggttaattcc tcccgtgtgg tgatatagcc ttgtagggaa
    22441 gggcgtgtca catacaacga gcctttctga ttgagaatgc ctaagttcac accggtaacc
    22501 gcacctgatg agttgccaaa gctcaccatt aagccgcggc gttgcaggca atccagcgac
    22561 cgttcccagg tgtctctgcc cacggaatcg tacaccacgc gcactttctt gccaccggtg
    22621 atctctttta accgctcgac cagattctct tcacgatagt taataacctg ccatgcgccc
    22681 gcttttagcg cactctgcgc tttttgcgcg gttcctacgg tgccgataag tttcgcgcca
    22741 agggcttttg cccactggca ggcaatcaag ccaacgccgc cagccgctgc gtggaacagg
    22801 aactgctcat cgggtttaat ttcataggtt ttgcgcagca gataataaac cgttaagcct
    22861 ttcaggaagg atgccgcagc ttgctcaaaa gaaattgccg caggcagaat cgccgcttta
    22921 tccgcattaa tgttatgcac agagctgtaa gcgcctaatg ccgattgtgc atagactacg
    22981 cgatcgcctg ccttaatatg ctttacacca ctgccgactt tactcacgat gcctgccgct
    23041 tcggtgccta atccgctggg tagcgatggc ggcgggtaaa ggccgctgcg gatatacgtg
    23101 tcgataaaat tgatgccgat ggctttattt tcgacctgga tttcattctc cgccggatcg
    23161 gcaggagtga actctacggc ttgaagtact tccgggccac cgtgcttgtg aaattcaatt
    23221 cgtgttgcca tgtgtcctcc agaacgtatc gtcagggtct gcttcatatg gtaaagtttc
    23281 gacccattct ttatctcggt aactccattc actatggcag gaaataaacc cttcaacaaa
    23341 cagcaggctg aaccccgcga acgcgatcca caagttgccg ggctgaaagt gcctccgcac
    23401 tcgatcgaag cggagcagtc ggtgttgggc ggtttaatgc tggataacga acgctgggat
    23461 gatgtagctg agcgtgtggt ggcagatgat ttttacaccc gcccacaccg tcatatcttt
    23521 actgaaatgg cgcgtttgca ggaaagcggt agtcctatcg atctgattac ccttgcggaa
    23581 tcgctggaac gccaggggca actcgatagc gtcggtggtt ttgcttatct ggcagagctg
    23641 tcaaaaaata cgccaagtgc ggcgaacatc agtgcttatg ctgacatcgt gcgtgaacgt
    23701 gccgttgttc gcgagatgat ttcggttgcg aatgagattg ccgaagccgg ttttgatccg
    23761 caggggcgca ccagcgaaga tctgctggac ctggctgaat cccgcgtctt taaaattgcc
    23821 gaaagtcgtg caaacaaaga cgaagggccg aagaacatcg ctgatgtgct cgacgccacg
    23881 gtggcgcgta ttgagcagtt gtttcagcag ccacacgatg gcgttaccgg ggtaaacacc
    23941 ggttatgacg atctcaacaa aaaaaccgct ggcttgcagc cgtcggattt gatcatcgtc
    24001 gccgcgcgtc cgtcgatggg taaaacaaca tttgcgatga acctcgtcga aaacgcggcg
    24061 atgttgcagg ataaaccagt acttatcttc tcgctggaga tgccatcaga acagattatg
    24121 atgcgttctc tggcgtcgct gtcgcgcgtt gaccagacta aaatccgtac cgggcagctc
    24181 gatgatgaag actgggcgcg catttccggc accatgggta ttttgctcga aaaacgcaat
    24241 atctatatcg atgactcctc cggcctgacg ccaacggaag tgcgttcccg cgcgcgccgt
    24301 attgcccgtg aacacggcgg catcgggctt atcatgatcg actacctgca actgatgcgc
    24361 gtaccggcgc tttccgataa ccgtacgctg gaaattgcag aaatctcccg ctcgctgaaa
    24421 gcactggcga aagaattgaa cgtgccggtg gtggcgctgt cccagttgaa ccgttctctg
    24481 gaacaacgtg ccgacaaacg cccggtcaac tccgacctgc gtgaatctgg ctctatcgag
    24541 caggatgcgg acttgatcat gtttatctat cgtgatgagg tgtatcacga aaacagtgat
    24601 ttaaaaggca tcgcggaaat tattatcggt aaacaacgta acggcccaat cgggacggta
    24661 cgcctgacct ttaacggtca atggtcgcgc ttcgacaact atgcggggcc gcagtacgac
    24721 gacgaataat aattatttta tgaattaggt aattaaagca aacacttatc aaggaacaca
    24781 aatgcaagcg gcaactgttg tgattaaccg ccgcgctctg cgacacaacc tgcaacgtct
    24841 tcgtgaactg gctcctgcca gtaaaatggt tgcggtggtg aaagcgaacg cttatggtca
    24901 cggtcttctt gagaccgcgc gaacgctccc cgatgctgac gcctttggcg tagcccgtct
    24961 cgaagaagct ctgcgactgc gtgcgggggg aatcaccaaa cctgtactgt tactcgaagg
    25021 cttttttgat gccagagatc tgccgacgat ttctgcgcaa cattttcata ccgccgtgca
    25081 taacgaagaa cagctggctg cgctggaaga ggctagcctg gacgagccgg ttaccgtctg
    25141 gatgaaactc gataccggta tgcaccgtct gggcgtaagg ccggaacagg ctgaggcgtt
    25201 ttatcatcgc ctgacccagt gcaaaaacgt tcgtcagccg gtgaatatcg tcagccattt
    25261 tgcgcgcgcg gatgaaccaa aatgcggcgc aaccgagaaa caactcgcta tctttaatac
    25321 cttttgtgaa ggcaaaccag gtcaacgttc cattgccgcg tcgggtggca ttctgctgtg
    25381 gccacagtcg cattttgact gggtgcgccc gggcatcatt ctttatggcg tctcgccgct
    25441 ggaagatcgt tccaccggtg ccgattttgg ctgtcagccg gtaatgtcat tgacctccag
    25501 cctgattgct gtgcgtgagc ataaagccgg agagcctgtc ggttatggtg gaacctgggt
    25561 aagcgaacgt gatacccgcc ttggcgtagt cgcgatgggc tatggcgatg gttatccgcg
    25621 cgccgcgccg tccggtacgc cagtgctggt gaacggtcgc gaagtgccga ttgtcgggcg
    25681 cgtcgcgatg gatatgatct gcgtagactt aggtccacag gcgcaggaca aagccgggga
    25741 cccggtcatt ttatggggcg aaggtttgcc cgtagaacgt atcgctgaaa tgacaaaagt
    25801 gagcgcttac gaacttatta cgcgcctgac ttcaagggtc gcgatgaaat acgtggatta
    25861 atcgttctgt aatatttgat tgtctgtgcc ggatgcggcg tgaatgcctt atccggccaa
    25921 taaaatctta ccaattcaat aagttgatat tctttcatgc tcttataaag gtcgtgcatc
    25981 tggcggatgt acgtttgtca tgagtctcac tctgttgcta attgccattc gcccctgaac
    26041 atcctctcga tcttcgcctt cttccggttt attgtgtttt aaccacctgc ccgtaaacct
    26101 ggagaaccat cgcgtgtttc aaaaagttga cgcctacgct ggcgacccga ttcttacgct
    26161 tatggagcgt tttaaagaag accctcgcag cgacaaagtg aatttaagta tcggtctgta
    26221 ctacaacgaa gacggaatta ttccacaact gaaagccgta gcggatgcgg aagcgcgcct
    26281 gaatgcgcag cctcatggtg cttcgcttta tttaccgatg gaagggctta acagctatcg
    26341 ccatgccatt gcgccgctgc tgtttggtgc ggaccatccg gtactgcaac aacagcgcgt
    26401 agcaaccatt caaacccttg gcggctcagg ggcattgaaa gtgggtgcag atttcctgaa
    26461 acgctacttc ccggaatcag gcgtctgggt cagcgatcct acctgggaaa accacgtagc
    26521 aatattcgcc ggggctggat tcgaagtgag cacttacccc tggtatgacg aagcgactaa
    26581 cggcgtgcgc tttaatgacc tgttggcgac gctgaaaaca ttacctgccc gcagtattgt
    26641 gttgctgcat ccatgttgcc acaacccaac gggtgccgat ctcactaatg accagtggga
    26701 ttccgtgatt gaaattctca aagcccgcga gcttattcca ttcctcgata ttgcctatca
    26761 aggatttggt gccggtatgg aagaggatgc ctacgccatt cgcgccattg ccagcgctgg
    26821 attacccgct ctggtgagca attcgttctc gaaaattttc tccctttacg gcgagcgcgt
    26881 cggcggactt tctgttctgt gtgaagatgc cgaagccgca ggccgcgtac tggggcaatt
    26941 gaaagcaaca gttcgccgca actactccag cccgccgaat tttggtgcgc aggtggtggc
    27001 tgcggtgctg aatgacgagg cattgaaagc cagctggctg gcggaagtag aagagatgcg
    27061 tactcgcatt ctggcgatgc gtcaggaact ggtgaaggta ttgagcacag agataccaga
    27121 acgcaatttc gattatctgc ttaatcagcg cggtatgttc agttataccg gtttaagtgc
    27181 cgctcaggtt gaccgactac gtgaagaatt tggtgtctat ctcatcgcca gcggtcgcat
    27241 gtgtgtcgcc gggttaaata cggcaaatgt acaacgtgtg gcaaaggcgt ttgctgcggt
    27301 gatgtaatgc aggaaagcag gctggagtta tccagcctgc agtgaaatta aactgtcgtc
    27361 gctttcactc ttgctttata gatgattttt ttgatgccat cgttctacgt gagaggtaat
    27421 aaaacgttgt tagttctaac ttgttaagtt tatcccaatg ttctggagta cttttccctg
    27481 ttttaccgtg tggaataata atttttgcac atctaaccat tattaaattt aataataagt
    27541 atcgggtttg ttacgataga atttttctat atcctcaaga atatgctttg ccttattgcc
    27601 tgtgactatc tgagattcaa ttatagtatc gttaaattct aaagttaaag agaactcttt
    27661 ttttccgctt tgtgtaagtt taaccttata ttgattacca atatcaaata aaaaggtcct
    27721 gtaatgagaa taatcacctt taacctcctc ggcaaaacag caaagcgtgc aaatatacat
    27781 acaatctttc ttacccctga ttttgttaac taaatcaata aatgcgtgaa aaactg
//
LOCUS       DAZUCC010000044        27475 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZUCC010000044 DAZUCC010000000
VERSION     DAZUCC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.044"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..299
                     /locus_tag="ACHY09_001670"
     CDS             <1..299
                     /locus_tag="ACHY09_001670"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFS3884655.1"
                     /translation="DRHQDHLAVYQASMVACRAIPQILGYETPSTWLSFMPQVFESVK
                     EEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVIHKMIL"
     gene            309..1505
                     /gene="yaiP"
                     /locus_tag="ACHY09_001671"
     CDS             309..1505
                     /gene="yaiP"
                     /locus_tag="ACHY09_001671"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS3884656.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            1540..2064
                     /locus_tag="ACHY09_001672"
     CDS             1540..2064
                     /locus_tag="ACHY09_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS3884657.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            2066..2839
                     /gene="yaiO"
                     /locus_tag="ACHY09_001673"
     CDS             2066..2839
                     /gene="yaiO"
                     /locus_tag="ACHY09_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFS3884658.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3024..3299
                     /gene="frmR"
                     /locus_tag="ACHY09_001674"
     CDS             3024..3299
                     /gene="frmR"
                     /locus_tag="ACHY09_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFS3884659.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3334..4443
                     /gene="frmA"
                     /locus_tag="ACHY09_001675"
     CDS             3334..4443
                     /gene="frmA"
                     /locus_tag="ACHY09_001675"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFS3884660.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            4535..5368
                     /gene="fghA"
                     /locus_tag="ACHY09_001676"
     CDS             4535..5368
                     /gene="fghA"
                     /locus_tag="ACHY09_001676"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFS3884661.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFAPIVSPSQVPWGQQAFTAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(5495..6034)
                     /gene="yaiL"
                     /locus_tag="ACHY09_001677"
     CDS             complement(5495..6034)
                     /gene="yaiL"
                     /locus_tag="ACHY09_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFS3884662.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDYLMW"
     gene            complement(6136..7347)
                     /gene="mhpT"
                     /locus_tag="ACHY09_001678"
     CDS             complement(6136..7347)
                     /gene="mhpT"
                     /locus_tag="ACHY09_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFS3884663.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGIGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(7523..8536)
                     /gene="mhpE"
                     /locus_tag="ACHY09_001679"
     CDS             complement(7523..8536)
                     /gene="mhpE"
                     /locus_tag="ACHY09_001679"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFS3884664.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(8533..9483)
                     /gene="mhpF"
                     /locus_tag="ACHY09_001680"
     CDS             complement(8533..9483)
                     /gene="mhpF"
                     /locus_tag="ACHY09_001680"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFS3884665.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(9480..10289)
                     /gene="mhpD"
                     /locus_tag="ACHY09_001681"
     CDS             complement(9480..10289)
                     /gene="mhpD"
                     /locus_tag="ACHY09_001681"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFS3884666.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10299..11165)
                     /gene="mhpC"
                     /locus_tag="ACHY09_001682"
     CDS             complement(10299..11165)
                     /gene="mhpC"
                     /locus_tag="ACHY09_001682"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFS3884667.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVKAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11183..12127)
                     /gene="mhpB"
                     /locus_tag="ACHY09_001683"
     CDS             complement(11183..12127)
                     /gene="mhpB"
                     /locus_tag="ACHY09_001683"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFS3884668.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12129..13793)
                     /gene="mhpA"
                     /locus_tag="ACHY09_001684"
     CDS             complement(12129..13793)
                     /gene="mhpA"
                     /locus_tag="ACHY09_001684"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFS3884669.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            13984..14817
                     /gene="mhpR"
                     /locus_tag="ACHY09_001685"
     CDS             13984..14817
                     /gene="mhpR"
                     /locus_tag="ACHY09_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFS3884670.1"
                     /translation="MQNNEQTAYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            14894..15976
                     /gene="lacI"
                     /locus_tag="ACHY09_001686"
     CDS             14894..15976
                     /gene="lacI"
                     /locus_tag="ACHY09_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFS3884671.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPQALADSLMQLARQISRLESGQ"
     gene            16099..19173
                     /gene="lacZ"
                     /locus_tag="ACHY09_001687"
     CDS             16099..19173
                     /gene="lacZ"
                     /locus_tag="ACHY09_001687"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFS3884672.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSKEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDISLLHK
                     PTTQISDFHVTTRFNDDFSRAVLEAEVQVYGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVLRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLTENLSVTLPSAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQTEVALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19225..20478
                     /gene="lacY"
                     /locus_tag="ACHY09_001688"
     CDS             19225..20478
                     /gene="lacY"
                     /locus_tag="ACHY09_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFS3884673.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            20544..21155
                     /gene="lacA"
                     /locus_tag="ACHY09_001689"
     CDS             20544..21155
                     /gene="lacA"
                     /locus_tag="ACHY09_001689"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFS3884674.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVHFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKHYYYKDYKVESSV"
     gene            complement(21258..22439)
                     /gene="cynX"
                     /locus_tag="ACHY09_001690"
     CDS             complement(21258..22439)
                     /gene="cynX"
                     /locus_tag="ACHY09_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFS3884675.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQNAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVFWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(22445..22915)
                     /gene="cynS"
                     /locus_tag="ACHY09_001691"
     CDS             complement(22445..22915)
                     /gene="cynS"
                     /locus_tag="ACHY09_001691"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFS3884676.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(22946..23605)
                     /gene="cynT"
                     /locus_tag="ACHY09_001692"
     CDS             complement(22946..23605)
                     /gene="cynT"
                     /locus_tag="ACHY09_001692"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFS3884677.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTSIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23715..24614
                     /gene="cynR"
                     /locus_tag="ACHY09_001693"
     CDS             23715..24614
                     /gene="cynR"
                     /locus_tag="ACHY09_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFS3884678.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(24616..24707)
                     /locus_tag="ACHY09_001694"
                     /pseudo
     CDS             complement(24616..24707)
                     /locus_tag="ACHY09_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24747..26030)
                     /gene="codA"
                     /locus_tag="ACHY09_001695"
     CDS             complement(24747..26030)
                     /gene="codA"
                     /locus_tag="ACHY09_001695"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFS3884679.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26020..27279)
                     /gene="codB"
                     /locus_tag="ACHY09_001696"
     CDS             complement(26020..27279)
                     /gene="codB"
                     /locus_tag="ACHY09_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFS3884680.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         6521 a         7504 c         7174 g         6276 t
ORIGIN      
        1 ctgaccgcca tcaggatcat cttgcagtct atcaagcctc aatggttgcc tgtcgcgcta
       61 ttccgcaaat tctcggctat gaaacaccaa gtacctggct ttcatttatg cctcaggttt
      121 ttgaatccgt gaaagaagaa tatttttctc ttaaactgac tgcgttaaaa aaacataaaa
      181 gtcagagcca gcgagattat atgcgcccgg agcgtctgcg tgcagttgct caatttcgcg
      241 ggcaacaggt caatagcgat ctgggtgaag gttttgttat tcataaaatg attctttgag
      301 caaattgcat gaaaacctgg atctttatat gtatggccgt agcaatattg ctatggtttc
      361 tgagtacgtt aaggcgtaag cccagccaaa agaaaggctg tattgacgcc attatacctg
      421 cttataacga aggcccgtgt ctggcgcagt cactggataa tctgctgcgt aacccttatt
      481 tctgccgggt aatttgcgtt aacgacggct ccacggacaa taccgaagcg gtcatggcgg
      541 aagtcaaacg caaatggggc gaccgcttta ttgccgtcac gcaaaaaaat accggtaaag
      601 gtggtgcgct aatgaatggc ctcaattacg ccacctgcga tcaggttttt ttaagtgatg
      661 ccgacaccta tgttccgccc gatcaagacg gaatgggcta tatgctggca gaaatagagc
      721 gcggtgctga tgccgtaggc ggcattccct ctactgcgtt gaaaggcgcg ggtctgttac
      781 cgcacatccg cgcgaccgta aagttgccga tgattgttat gaagcgcacg ctacagcagc
      841 tcctgggcgg cgcaccgttt attatcagcg gtgcctgcgg gatgttccgt actgatgtat
      901 tgcgtaagtt cggtttctct gatcgtacta aagtcgaaga ccttgatctc acctggacat
      961 tggtggcaaa cggctatcgt attcggcagg cgaatcgctg catcgtatac ccacaggaat
     1021 gcaacagccc gcgtgaggaa tggcgtcgct ggcggcgttg gattgtgggc tacgcggtct
     1081 gtatgcgcct gcataaaaga cttttattta gccgcttcgg tatcttcagt atatttccta
     1141 tgctgttggt tgtgctttat ggcgttggga tttatctcac tacctggttt aatgaattca
     1201 tcaccaccgg gccacatggt gtggtgttgg caatgttccc gcttatctgg gtcggcgtag
     1261 tttgtgttat tggtgcattt agcgcctggt ttcatcgttg ctggttgttg gtgcctttag
     1321 caccgctttc cgttgtgtat gtattattag cttatgccat ctggattatt tatggactta
     1381 ttgccttttt tactggacgc gaacctcagc gcgacaaacc cacccgctat tccgcactgg
     1441 tggaagcgtc aaccgcttat tcccaccctt ctgtcacagg aactgaaaaa ctatctgaag
     1501 cttaatgtta aagagaaaaa tattcagatt gcagaccagg tgattattga tgaaagtgcc
     1561 ggtgaggttg ttatcggcgc gaatacccgt atttgccacg gtgccgttat tcagggtccg
     1621 gtagtgattg gcgcaaactg cctgataggt aattatgcgt ttattcgtcc tggcacaata
     1681 atcagcaatg gcgtaaaaat tggttttgcc accgaaatta aaaatgcggt tattgaagcg
     1741 gaagcaacga ttggtccgca atgctttatt gccgactcgg tagttgcaaa ccaggcatat
     1801 ttgggcgcac aagtacgaac cagtaatcat cgtctggatg aacagcccgt gtctgttcga
     1861 actccagagg gaattatcgc taccggatgc gataaattag gttgttatat cgggcagcgt
     1921 tcacgccttg gcgtacaagt tattattttg cctgggcgaa ttatttcccc gaacacgcaa
     1981 cttggcccgc gcgtgattgt agaacgtaat ttacctaccg gaacttattc actccgacaa
     2041 gaacttatcc gtacaggaga ttaaaatgat aaaacggacg ttattagcgg cggcaatatt
     2101 tagcacattg cccgcttatg ccgggttaac ttccattacc gcgggctacg attttaccga
     2161 ttattctggc gatcatggca accgtaattt agcgtatgct gaactggtgg cgaaagttga
     2221 aaacgcaacg ctgcttttta atctttccca ggggcgtcgt gattatgaaa ctgaacattt
     2281 caatgccact cgcggtcagg gtgcggtctg gtataaatgg aataactggc tgacaacccg
     2341 aacgggtatt gcctttgcgg ataatacgcc ggtctttgcc cgccaggatt ttcgtcagga
     2401 tattaacctg gccctgttgc caaaaacgct tttcacgacc ggttatcgct acactaaata
     2461 ttacgatgat gtcgaagtcg atgcctggca aggcggcgta tccctctata caggcccggt
     2521 gatcaccagc tatcgctata cccattatga ctccagcgat gcaggtggta gttacagcaa
     2581 tatgatttcc gtgcgtctga atgacccgcg cggcactggt tatacgcaac tatggctaag
     2641 ccgcggaaca ggtgcttaca cctatgactg gacgccagaa acacgctacg gcagcatgaa
     2701 gagcgtcagt ttgcaacgta ttcaaccgct gactgagcaa cttaatctcg ggctgacggc
     2761 aggtaaagtg tggtacgaca ccccaaccga tgattataac ggtctgcaac ttgcagccca
     2821 tctgacctgg aaattctgat tccttctgcc gcccgctatc cggggcggcc ttccctgtcg
     2881 attaggcccc tctttacttt ttgttatccg actacattca ccggataaat tttattctcc
     2941 agtgttatat actatagagg ggtatgcatt gacatataac atacccccct atagtatatt
     3001 gcgtgcagat gatgaggtgc gaaatgccca gtactccgga agagaagaaa aaggtcctta
     3061 ctcgagttcg tcgtattcgg gggcagattg atgctctgga acggtcgctg gagggtgatg
     3121 ccgaatgccg tgccatactc caacagatcg ctgccgttcg gggcgcggct aacggactga
     3181 tggcagaagt gcttgaaagc catatccggg aaacgtttga ccgaaatgac tgctacagcc
     3241 gcgaagtcag ccaatccgtt gacgacacta ttgaactggt tcgtgcctat cttaaatagc
     3301 tgaatctatt accatattga ggaagagcga gagatgaaat cacgtgctgc cgttgcattt
     3361 gctcccggta aaccgctgga aatcgttgaa attgacgttg caccaccgaa aaaaggtgaa
     3421 gtgctgatta aagtcaccca taccggtgtt tgccataccg acgcatttac cctctccggc
     3481 gatgacccgg aaggtgtctt cccggtggtt ctcggtcacg aaggggccgg ggttgtagtt
     3541 gaagtcggtg aaggcgtaac cagcgtcaaa cctggcgacc atgtgatccc gctttacacc
     3601 gcagagtgcg gcgagtgtga gttctgtcgt tctggcaaaa ccaacctgtg tgtcgcggtt
     3661 cgcgaaacgc agggcaaagg cttgatgcca gacggcacca cccgtttttc ttacaacggg
     3721 cagccgcttt atcactacat gggatgctca acattcagtg aatacaccgt ggtcgcggaa
     3781 gtgtctctgg ccaaaattaa tccagaagca aaccatgaac acgtctgcct gctgggctgt
     3841 ggcgtgacca ccggtattgg cgcggtacac aacacagcta aagtccagcc aggtgattct
     3901 gttgccgtgt ttggtcttgg cgcgattggt ctggcagtgg ttcagggcgc gcgtcaggcg
     3961 aaagcgggac ggattatcgc tatcgatacc aacccgaaga aattcgatct ggcgcgtcgc
     4021 ttcggtgcca ccgactgcat taacccgaat gactacgaca aaccgattaa agatgtcctg
     4081 ctggatatca acaaatgggg tatcgaccat acctttgaat gcatcggtaa cgtcaacgtg
     4141 atgcgtgcgg cgctggaaag tgcgcaccgc ggctggggtc agtcggtgat catcggggta
     4201 gctggttctg gtcaggaaat ctccacccgt ccattccagt tggtcaccgg tcgcgtatgg
     4261 aaaggttccg cgtttggcgg cgtgaaaggt cgttcccagt taccgggtat ggttgaagat
     4321 gcgatgaaag gcgatatcga tctggaaccg tttgtcacgc ataccatgag cctggatgaa
     4381 attaatgacg cctttgacct gatgcatgaa ggcaaatcca ttcgaaccgt aattcgttac
     4441 tgatttcccg caggtttacc cgtccagttc agacggggtt cttaatactc tccccgggca
     4501 gccgtccggg ggattaaccg tgagataatg actgatggaa ctcattgaaa aacatgccag
     4561 ctttggcggc tggcaaaatg tgtatcggca ttattcccca tcactgaaat gtgaaatgaa
     4621 tgtcggcgtc tatctcccgc caaaagccgc aaatgaaaaa ttgccggtgt tgtactggct
     4681 ttcaggcctg acctgcaacg agcagaattt cattactaaa tcggggatgc agcgttacgc
     4741 ggctgagcac aacattattg ttgttgcgcc agacaccagt ccgcgaggca gtcatgtcgc
     4801 agatgccgac cgttacgatc tcgggcaagg tgccgggttt tacctgaacg caacgcaagc
     4861 gccgtggaat gaacattaca aaatgtatga ctatatccgc aacgagctgc cggatttagt
     4921 gatgcaacat tttccggcaa cgaccagaaa gtctatctca ggtcattcta tgggcgggct
     4981 gggcgcgctg gtgctggcgt tacgtaaccc agatgaatat gtcagcgtct cggcgtttgc
     5041 gcccattgtc tccccatcgc aagtaccgtg gggacagcaa gcctttaccg catatcttgg
     5101 tgaaaataaa gatgcctggt tggattacga tccggtgagt cttatttcac aaggtcaacg
     5161 cgttgcggaa ataatggttg atcaggggtt gagtgatgat ttttacgcag aacagctgcg
     5221 gactccaaat cttgaaaaga tctgccagga gatgaatatc aaaacgttaa tccgttatca
     5281 cgagggttat gatcacagct attattttgt ctccagtttt attggcgagc atattgcgta
     5341 ccacgccaat aaactgaata tgcgttgagc atccgcgcac gcatgccgga tgcggcgtaa
     5401 acgccttatc cggcctacat ttcacccgta agccgtaggc ctgataagac gcgaaaagcg
     5461 tcgcatcagg catcactgca cggcttatct atgtttacca catcaaataa tcaggcactt
     5521 tgaagtcggc atacggatcg tcttcatcct gctcttcggc gctgagcgcg ctgtgcaaca
     5581 caatactgct ggcatcacgc tgagcaattt tatcggcaac actggcggga ataatggcgt
     5641 attcaccttc gctattgtta tcgaccaaca agcgggcaat cgccagacga ccattaatca
     5701 actgcgcctg tgtgagctta tcgacaaaaa tcttcttaat cagattgccg tcagtgaagt
     5761 taaaaccaat atcgccattg gcaatggtga ttcggttcat ttcaatgagc tgcttcacct
     5821 gggctttata ttctttcgcc aacgccgctt gtttttgctg ttcgctaagc tgtttatcac
     5881 gctcaagctg tgccttttta ttttcttcta ccgccgcccg agcttcacgc gcctgaacgc
     5941 gcgatttttt cgccgttctc tcgaccttcg ccgctttttt gctggtgact aatcctgctt
     6001 tgagcaactg ctcttgtaag gtaagttttg ccatctttgt ttccaaaccc gctgaataat
     6061 ttttagcgag tatacctgta aacgccgtag gtgtattggg ggcaagtccc tttcgcccct
     6121 gcttaataag gcgcgtcagg catcggcgca cggctgcatt cgtgatctcc ggctcatcaa
     6181 aataaatact gccaggccag caacaagaat acccggcgca gaagcggcca ttacgccaac
     6241 cgtgccaatg cctaatgcca gcattttccc ggccagtaac ggaccgctca tagcccccag
     6301 acgccctacc gccacggctg ttcccacacc tgttgcgcgg atctgcgaac tgtaaaacaa
     6361 cggtgccagg gcatacaaaa cgctttgccc acctgtcgca aacaaccccg cgacgaatcc
     6421 cgccagcaac ataccgttaa atgacgacac cgttcccagc gccagcagcg aagctaacat
     6481 gccgctataa atcagtagcg acatggttac tggacgcagc ttatccatca atgcgcccaa
     6541 cattaacgtc ccgcttgccg cccccatttg cagtgcaaac atcacccctg ccgcctgcga
     6601 tggctggaat ccttgctcca ccaaaagcag cggtagccag ttgatcaaca tgtagaccac
     6661 cagcagagtg aagaaataac acaaccacag cagcagcgtc gcggttgccg tttctggcgc
     6721 aaataacgca cgcagtggtg gcgcagcctg tttttcgcca gcgaacaccg ccgactccgg
     6781 cagccagcgc attaatagcg gcaccagaat caacggcacc acaccaccta cccaaaacac
     6841 cgtttgccat gctaagtttg cccccgcgaa acccagtgtc gccgccagcg ccgcgccaat
     6901 ggggacaccg caatacatca ggctcactgc cgtcccacga aaacgtggac ccgcggcttc
     6961 agacgtcagg gcgatcagat tcggcaacgc cgcccccagc ccgacaccgg tcatcagccg
     7021 cgcaaagacc agtgagggga aatcccaggc aatcgccgtt gccagtgaga acaaaccaaa
     7081 cagcgcaact gagccaatca aaatgcgctt gcgaccataa cggtccgcca gcattccgcc
     7141 aaccaacgcg ccgggtagca aaccgagtat tccggcgctg aatatccagc ccatttgcat
     7201 tttatcgagt gcgaaagcct gggcgatgcc acccgccgca atgccagccg cctgaagatc
     7261 cagtccttcc atcagagcga ccaaaaaaca aagcccgatg gtcagcatca ggcgggttga
     7321 agatgatgaa ggggtacgag tcgacataag atgaacctcg ttgcgagtgc aggtgaatcg
     7381 cggctgatac attgtcagcc gcgtaatcag aaccatttaa tgactgccta cgggcgaaat
     7441 ataggccgga taaggcgttt acgccgcatc cggcagtcgt gctgggatgg gcatacaaaa
     7501 tgcacgttct cggcagtcat cattatttgt tgttgcgcag atccagcgcc acgtcgacga
     7561 tcatatcctc ctggccgcca accatccggc gtttgcccag ctcaacgaga atatccaccg
     7621 cgcttaagcc ataacgcgcc gccgccgttt cacagtgacg caggaagctc gagtaaacac
     7681 cagcgtatcc cagcgccagc gtttcgcgat cgactcgtac cggtcgatcc tgcaacggac
     7741 gcaccaggtc gtcagcggca tccattaacg catagagatc ggtcccatgc tgccaaccca
     7801 gtttatccgc ggcggcaata aaaacttcca gcggcgcgtt acctgcgccc gcgcccattc
     7861 ccgcgaggct ggcgtcgatt cggtcgcagc cctcttccac cgccgcgata gagttcgcca
     7921 cgccaagact caggttatgg tgagcgtgta tgccggtttg cgtttctggt ttcagcactg
     7981 ctttcagagc gcggaaacgg tcacggatat cgctcatgtt catcgcaccg ccagaatcca
     8041 ccacataaat acaggtcgca ccgtagcctt ccatcagctt tgcctgcttt gcgagattct
     8101 ccggcgtggt catatggctc atcatcagaa aaccaacggt gtccattccg agctcgcggg
     8161 catactgaat atgctgggcg gaaacatcag cttcggtaca gtgcgtagcc acacgaacca
     8221 cccgcgcgcc agcctgccag gcatttttca gatcgtgaat agtgccgatt ccaggcagca
     8281 acaacgtcgc gattttggcg tgcttcacca catccgccgc cgcttcaatc cattcaaggt
     8341 cgctatgtgc gccgaaacca tagttaaagc tggaaccttg caaaccgtcg ccgtgggcca
     8401 cttcaatcga atccacgcgg gcatcgtcca gtgctttggc aacctggcga acgttttcca
     8461 atgaatactg atgacgaatg gcgtgcatac cgtcacgcaa cgtgacgtcc gagatataaa
     8521 gttttttacc gttcatgccg cttctcctgc cttgcgcgcc agtgactggg ccattttttc
     8581 cgctgtcgcc agcgcactgg aagtcataat gtcgaggttg cctgcatagg caggcagata
     8641 atgcgctgcg ccttcgactt ccagccagac cgccgttttc agtccggaga actgccccac
     8701 gcccggcaaa ttaaccggtt tatcctgcgg gataacttca aactgcacgc gctgtttcag
     8761 gcgataaccc ggtacgtaag cctgcaccgc ctcagccatt tcattgattg aggcttcgat
     8821 atcatcttgt gaagcttcgt cgctcaatac atacaccgtg tcacgcatca tcaacggtgg
     8881 ctctgctggg ttaagcacaa taatcgcctt cccttttgcc gcgccaccca ccacttcaat
     8941 ggcgcgcgaa gtggtttccg taaattcatc gatattggca cgcgtgccag gtccggcaga
     9001 tttactggca atagaagcga taatttcggc gtaatgaaca cgcgccacgc gtgaaactgc
     9061 cgccaccatt ggaatggtgg cctggccgcc gcaggtgacc atgttgacgt tcagttgagc
     9121 gacgttctcc tcgaggttaa cgaccggcac gcagtagggg ccgatggcag caggcgtcag
     9181 gtcaattaag cgaatatccg gtttcgcttc gcgcaaagcg gcatcgtttt tcacatgcgc
     9241 acccgcgctg gtcgcatcaa atacaatgtc gatatcagca aattcaggca tgttcatcag
     9301 gccgatcacc ccttcatggg tggtggcgac gcccatacgt ctggcacgcg ccagaccgtc
     9361 ggactgagga tcaatgccga ccatcaccgc catctccaga tgctggccgt gacgcaaaat
     9421 tttaatcatc agatcggtgc caatgttgcc tgaaccgata atggcgactt tacgcttact
     9481 catgacagac ttccttttgg ggctgcgctt gaaaatgtcg cagcaactga acctatgcct
     9541 tcaatatggg cttcaaaacg atcgcccgca ttcaccgcca ccatcggacc taatgccccg
     9601 gtaagaatga tatctccggc gcgcagcggt tcacccaggc tggccatttt gcgtgccagc
     9661 cagacggcgg cattaagcgg atgtcccagg cattcgctgc cgcgcccgct ggaaacctct
     9721 tcgttattac gcgtcatctt catggcgcag tttttcaggt ctaaccccgc cggacgttgc
     9781 gccggaccgc cgatgacata caccccgcag gaagcgttat ctgccacggt atcgacaaac
     9841 tgaatcgacc agtcgcgaat gcggctcccc accacttcca gcgccggaag tacccattca
     9901 atggcgttat acaattcgtc gaaggtgata tcggttgcgg gtagatcgcg gttcaacacc
     9961 agtgcaatct ccgcttcaat gcggggttgc agaacacggg aaaaaggaat ggtttcgtta
    10021 tcgccataac acatgtcggc aaataatgtc ccgaaatccg gttgatcaac gcccagttgt
    10081 tgctgcactt tcggatgcgt cagccccact ttgcgcccca ccactcgccg tccctgagcg
    10141 acgtcgtgtt gcacattgat gtgctgaatg gcgtaggccg cttcagcgtt atcgatacca
    10201 atcagatcgc gcagcggcgc aatcgcttcg ccctgctctg cggcgcgacg taaatccgcc
    10261 gccagttgct caagagtatg cttcgtcatg accgttcctt aagggcgtgc gaggaaattc
    10321 agcaccagtt gattgaaagc gtcggcatgt tcccactgcg cccagtgacc acagtcgcgg
    10381 aagatatgca gttcagaacc ggcaatgccg gacagcagac gcagacccgc atccatcggc
    10441 acaaagcggt cgttgcgccc ccagacaatc agggtttgcg ctttgatttc cgccagacgt
    10501 gggccaaaat ccgggaactg tttcggattg gcttccaggc tcttaacgaa gttttccagg
    10561 tgatcgcggc gcgacagcat attattcagg cgcgcttcaa acagggcgtc ggtcaaatcg
    10621 ctggtatcaa aaacgaagat atccatcatc agcttcaggt tttcgatagt cggctgacga
    10681 taaagctgat tcagtcgctt aataccttcg gttggcatcg gcgtaaacaa actcatgccg
    10741 cccgtaccgc cgcccatcag caccagtttg ccgacgcgct ccggccagtt aagggtgaac
    10801 gccacagaac tgtggccgcc catcgagttg cccagcaggt ggattttggc gatatccagt
    10861 tgatccacca cgcttttcag gattcgcgca ttaagatccg atcgtgaacc actattaacg
    10921 atcgaatcgc tcttacccca acccggacaa tccagcagga tcacccgata gcccgccttt
    10981 accagcggat cgatattgcg gctgaagttc gcccagccag tagcacccgg gccggaacca
    11041 tgcagcagga caacagtttc gtcgccttgt ccgcagtcat taaaatggat gcgcagcgtt
    11101 ttacccgctt cttctacatt cagaaaacgg ctggtggcgg cttcggtttg tggctgataa
    11161 ctcatcatct tctcctgcat attcagttct ctgttctggc gcttaacgag ccaaatccgg
    11221 caatccactc cgggattggg cggtaataac gcccttcgct acgccagttg ccaaacgcag
    11281 aaatagcggc aaaagcggcg acccaggttt tgatttcatg tgtcgacttt ccggcaatgg
    11341 cggaaagctc ttcgttactg acggcatcca gttcctgtat gcgtccctgc tccagcaaag
    11401 tcatgaactg gttatcccaa atcgggttga gcggatgcag cgttctctga tcctcaacaa
    11461 acttctcagc ggcgctaatc acccgttgct gacgcaattc gcgctcactg gcgggtaaat
    11521 ctttcccgct ccccaacaga cggtcgcgca tatgggcatc ggctttcgcc agttctggca
    11581 ccggcggctg atgggaaagc ccaccggaac ccaggaacag cacgcgttta ttgagagtac
    11641 tggtgaaacg tccaatggct tcccccaaca tgcgggtacg ctggaaaccg ggcaacggcg
    11701 tggcgacacc gttgatgaac acaggcagaa ccggcacctt atccagccca ccgagcagga
    11761 actccagcgg ctgggcgaac ccgtggtcca cctgcataca gtaagaaacg gcaagatcga
    11821 ttccgctctt catgacggca tgcgcacagg cctccgccag ctcaacaggc acgggcagct
    11881 ctcctgccgc actaccgaaa tcaccaattg ccgtcgctcc aacgcctaaa cagaacggtg
    11941 gcatcacgtc atagaaaaag ccgttgtagt gatctggcgc aaacagcacc accaactcag
    12001 gggagaaagc cgcaatacgc tcgcgggcgc tggcaatcac gccattgacc tcatcgagca
    12061 cctcttgcgc cgggtcgaca tatcccacca gcggcgagtg ggaaagacag tgaagataag
    12121 cgtgcatatc aggctacctt ttcgacagaa acgtcggcat cagggcgggt cagcgtcatt
    12181 accgacgcca gtttattcag cgtattaccc agagtttgcg gaatggcggt ggcggcaaca
    12241 aagcgatccg ggcgcatcac caccagcgaa gcattgtgtt gcgcgaacca gctacgcagg
    12301 cgaccttgcg tatcgcccac gcgtagtacg ccgtcgtggt tatcctgtgc ggtatgaatt
    12361 tgcacttccg gcaccacctg aatgaagcgt gtgcccaacg cgcgccactg ctggatttgc
    12421 tcgtcgctca tcccccacag tggattgcat ccccagccaa ttaccgcgaa gttcgcgccg
    12481 atcgcgttat cgagcagcgt cacgtcgccg ttttccagcg tgactttcgg ctgaataaac
    12541 atcttgccga ccggagagtg cttcgcttcg ccctcacgca ccagcgcacc gccgtaatat
    12601 tgcggcatcg gcttgaagcg catttcgagg aagtagcgtt ttactggcgg cagataattc
    12661 aacagccagg aaacgccgtc acgtaacgta ccctgccagc gtttcggcgg agccagcacg
    12721 ttgcccgccg tcacggacag gtcaatcatc gctttggcgt gatcgcgacg ttcttgttga
    12781 taggtatcga gcagcgcatc gcgggctttc ccctggataa ccaacgccag tttccatgcg
    12841 aggttaaagg cgtcgcgcat accactgtta tagccctgcc cctgccacac cggcatgatg
    12901 tgcgcggcat cgcccgccag cagtacgcga tcaatacgaa aacgttgcgc cagtcgcgcg
    12961 ttgtgggtgt agacacgctg gcgaatcaat tcaacattgt ccggattagg cagcactttg
    13021 cttaacagct tgcgcatatt ttgcggctca cgcagctgtt cttcggtttc tcccggcatc
    13081 accataaatt caaagcgacg taccgcatga ggcagcgcgg cagaaacata cgggcgcacc
    13141 ggatcacaac acaaatagat atgcggcgta cttaacggat cgttggcgat atctaccaca
    13201 atccactgat ttggcgcagt tttaccttca aacggcacat tcagggtgcg acggacaaaa
    13261 cttgccccac catcacaggc caccagccat tgggctttga ccgtttcccg ctgcccttct
    13321 gccgttttca ggtgcaaggt cacttcgtca tcttgctggc tgaaggcctc cagctcgcgg
    13381 gaaaacaggc agcgcacatt cggaaaacgc gacaatcctt ccagcatcac cgcatcgacc
    13441 tgaggctgaa taaaggcgtt acggcgcggc cagccaaatt catcggtcat tggctgaata
    13501 tcagcaaaac agcgaccttt tggggtgaga aaacgcatcg cgtgccacgg cgtagtgtgc
    13561 ggcagaacat tatcgaccag gccgaccgac tgcatggtgc gcagcgcctc gtcatcaata
    13621 ccaatcgcac gcgggtagtc gatcaactta tcgagtttct ccaccaccag cacgtcaatg
    13681 cccatctggc cgagatagtt cgccatcatc agcccgaccg gaccggcacc agcgatcgcc
    13741 acctgaacgc tatggttaac agcaggctgg atgtcaggat gttgtattgc catttcagta
    13801 cctcacgact cggacaaaat gccgttgcgc gcacagtacg gcgcaactta ttttgttaaa
    13861 aacatgtaaa tgatttttta ttgtgcgctc agtataggaa gggtgttttc ggctacaatc
    13921 aaaacatgcc cgaatgtgca ccaagtgcac aacgttgttt taactataga aatgtcaatt
    13981 aatatgcaga acaatgagca gacggcatac aaaaccgtgc gcggcttaac ccgcggtcta
    14041 atgttattaa atatgttaaa taaacttgat ggcggtgcca gcgtcgggct gctggcggaa
    14101 ctcagcggcc tgcatcgcac cactgtgcgg cgactgctgg agacgctgca ggaagaggga
    14161 tatgtccggc gtagtccctc cgatgatagt ttccgtttga ccatcaaagt gcggcaatta
    14221 agcgaaggat ttcgtgacga acagtggatt tctgcactgg cggccccact gctgggcgat
    14281 ctgttgcgcg aagtggtatg gccgacagat gtgtccaccc tggatgttga tgcaatggtg
    14341 gtacgcgaaa ctacccatcg tttcagccgc ttatcttttc accgggcaat ggtcgggcga
    14401 cggttaccgc ttctgaaaac cgcctcgggc ctgacctggc tggccttttg cccggaacaa
    14461 gaacgcaagg aattaatcga gatgttagcc tcccgccccg gtgatgacta tcaactggca
    14521 cgggaaccgt taaagctgga agccattctg gcgcgcgcgc gcaaagaggg gtacggacag
    14581 aactaccgcg gctgggatca agaagaaaag atcgcctcta ttgccgtacc gctgcgcagt
    14641 gaacaacggg tgattggctg tctgaatctg gtgtatatgg cgagcgcaat gaccattgaa
    14701 caggcagcgg aaaagcatct tccggcgcta caacgggtgg caaaacagat cgaagaaggg
    14761 gttgaatcgc aggctattct ggtggccgga cggcgaagcg gcgtgcattt acgttgacac
    14821 catcgaatgg cgcaaaacct ttcgtggtat ggcatgatag cgcccggaag agagtcaatt
    14881 cagggtggtg aatgtgaaac cagtaacgct atacgatgtc gcagagtatg ccggtgtctc
    14941 ttatcagacc gtttcccgcg tggtgaacca ggcctgccac gtttctgcga aaacgcggga
    15001 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
    15061 ggcgggcaag cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
    15121 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
    15181 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
    15241 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
    15301 ggaagctgcc tgcgctaatg ttccggcttt atttcttgat gtctctgacc agacacccat
    15361 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
    15421 attgggtcac cagcaaatcg cgctgttagc aggtccatta agttctgtct cggcgcgtct
    15481 gcgtctggcg ggctggcata aatatctcac acgcaatcaa attcagccga tagcggaacg
    15541 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
    15601 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
    15661 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
    15721 cgaagacagc tcgtgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
    15781 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
    15841 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc cttccgccca atacgcaaac
    15901 cgcttctccc caggcgttgg cagattcttt aatgcagctg gcgcgacaaa tttcccgact
    15961 cgaaagcggg cagtgagcgc aacgcaatta atgtgagtca gctcactcat taggcacccc
    16021 aggctttaca ctttatgcgt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    16081 ttcacacagg atacagctat gactatgatt acggattcac tggccgtcgt attacaacgt
    16141 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcggcaca tccccctttc
    16201 gccagctggc gtaatagcaa agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    16261 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
    16321 ctggagtgcg atcttcctga cgccgatact gtcgtcgtcc cctcaaactg gcagatgcac
    16381 ggttacgacg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt
    16441 gttcccgcgg agaatccgac aggttgttac tcgctcacat ttaatgttga tgaaagctgg
    16501 ctacaggaag gccagacgcg aattattttt gatggcgtta attcggcgtt tcatctgtgg
    16561 tgcaacgggc gctgggtcgg ttacggccag gacagccgtt tgccgtctga atttgacctg
    16621 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtactgcg ctggagtgac
    16681 ggcagttatc tggaagatca ggatatgtgg cgaatgagcg gcattttccg tgacatctcg
    16741 ttgctgcaca aaccgaccac acaaatcagc gatttccatg ttaccactcg ctttaatgat
    16801 gatttcagcc gcgcggtact ggaggcagaa gttcaggtgt acggcgagct gcgcgatgag
    16861 ctgcgggtga cggtttcttt gtggcagggt gaaacgcagg tcgccagcgg caccacgcct
    16921 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac gctacgtctg
    16981 aacgtcgaaa acccggcgct gtggagcgcc gaaatcccga atatctatcg tgcggtggtt
    17041 gaactgcaca ctgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
    17101 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgcggc
    17161 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
    17221 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
    17281 aaccatccgc tgtggtacac cctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
    17341 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggtta
    17401 ccggccatga gcgaacgcgt aacgcgaatg gtgctgcgcg atcgtaatca cccgagtgtg
    17461 atcatctggt cgctggggaa tgaatcgggc cacggcgcta atcacgacgc gctgtatcgc
    17521 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
    17581 tccgcaaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gccctttccg
    17641 gctgtgccga aatggtccat caaaaaatgg ctttcgctgc ctggagaact gcgcccgctg
    17701 atcctttgcg aatatgccca cgcgatgggt aacagtcttg gcggcttcgc taaatactgg
    17761 caggcatttc gtcagtaccc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
    17821 tcgctgatta aatatgatga aaacggcaac ccgtggtcag cttacggcgg tgattttggc
    17881 gatacgccaa acgatcgcca gttctgcatg aacggtctgg tctttgccga ccgcacgccg
    17941 catccggcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
    18001 cgaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
    18061 tggtcggtgg cgctggatgg caagccgctg gcaagcggtg aaatgcctct ggatgttgct
    18121 ccacaagata aacagttgat tgaactgcct gaactaccgc agccggagag cgccggacaa
    18181 ctctggctta cggtacacgt agtgcaaccg aacgcgaccg catggtcaga agccggacac
    18241 atcagcgcct ggcagcaatg gcgtctgacg gaaaacctca gcgtgacact cccctccgcg
    18301 ccccacgcca tcccgcaact gaccaccagc gaaacggatt tttgcatcga gctgggtaat
    18361 aaacgttggc aatttaaccg ccaatcaggc tttctttcac agatgtggat tggcgatgaa
    18421 aaacaactgc tgacgccgct gcgcgatcag ttcacccgcg caccgctgga taacgacatt
    18481 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tagaacgctg gaaggcggcg
    18541 ggccattacc agaccgaagt ggcgttgttg cagtgcacgg cagatacact tgccgacgcg
    18601 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
    18661 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtagcg
    18721 agcgatacac cgcatccggc acggattggc ctgacctgcc agctggcgca ggtcgcagag
    18781 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
    18841 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
    18901 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
    18961 gacttccagt tcaatatcag tcgctacagc caacaacaac tgatggaaac cagccatcgc
    19021 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
    19081 ggtggcgacg actcctggag cccgtcagtg tcggcggaat tccagctgag cgccggtcgc
    19141 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc
    19201 gtatttcgcg taaggaaatc cattatgtac tatttaaaaa acacaaactt ttggatgttc
    19261 ggtttattct ttttctttta cttttttatc atgggggcct acttcccgtt tttcccgatt
    19321 tggctacatg acatcaacca tatcagcaaa agtgatacag gtattatttt tgccgctatt
    19381 tctctgttct cgctattatt ccaaccgctg tttggtctgc tttctgacaa actcgggctg
    19441 cgcaaatacc tgctgtggat tattaccggc atgttagtga tgtttgcgcc gttctttatt
    19501 tttatcttcg ggccactatt acaatacaac attttagtag gatcgattgt tggtggtatt
    19561 tatcttggct tttgttttaa cgccggtgcg ccagcagtag aggcatttat cgagaaagtc
    19621 agccgtcgca gtaatttcga atttggtcgc gcgcggatgt ttggctgtgt tggctgggcg
    19681 ctgtgtgcct cgattgtcgg catcatgttc accatcaata atcagtttgt tttctggctg
    19741 ggttctggct gtgcactcat cctcgccgtt ttgctctttt tcgccaaaac ggatgcgccc
    19801 tcttccgcca cggttgccaa tgcggtaggt gccaaccatt cggcatttag ccttaagctg
    19861 gcgctggaac tgttcagaca gccaaaactg tggtttttgt cactgtatgt tatcggcgtt
    19921 tcctgcacct acgatgtttt tgaccaacag tttgctaatt tctttacttc gttctttgcc
    19981 acaggtgaac agggtacgcg ggtatttggc tacgtaacga caatgggcga attacttaac
    20041 gcctcgatta tgttcttcgc gccgctgatc attaatcgca tcggtgggaa aaacgccctg
    20101 ctgctggctg gcactattat gtctgtacgt attattggct catcgttcgc cacctcagcg
    20161 ctggaagtgg ttattctgaa aacgctgcat atgtttgaag taccgttcct gctggtgggc
    20221 tgctttaaat atattaccag ccagtttgaa gtgcgttttt cagcgacgat ttatctggtc
    20281 tgtttctgct tctttaagca actggcgatg atttttatgt ctgtactggc gggcaatatg
    20341 tatgaaagca tcggtttcca gggcgcttat ctggtgctgg gtctggtggc gttgggcttc
    20401 accttaattt ccgtgttcac gcttagcggc cccggtccgc tttctctact gcgtcgtcag
    20461 gtgaatgaag tcgcttaagc aattaatgtc ggatgcggcg cgagcgcctt atccgaccaa
    20521 catatcagga cggaatgatc gcaatgaaca tgccaatgac cgaaagaata aaagcaggca
    20581 agctatttac cgatatgtgc gaaggcttac cggaaaaaag acttcgtggg aaaacgttaa
    20641 tgtatgagtt taatcactcg catccatcag aagttgaaaa aagagaaagc ctgattaaag
    20701 aaatgtttgc cacggtaggg gaaaacgcct gggtagaacc acccgtccat ttctcttacg
    20761 gttccaacat ccatataggc cgcaattttt atgcaaattt caatttaacc attgtagacg
    20821 actacacggt aacgatcggt gataacgtat tgattgcacc caatgttact ctttccgtta
    20881 cgggacaccc tgtacaccat gaattgagaa aaaacggtga gatgtactct tttccgataa
    20941 cgattggcaa taacgtctgg atcggaagtc atgtggtcat taatccaggc gtcaccatcg
    21001 gggataattc tgttattggc gcgggtagtg tcgtcataaa agacattcca ccaaacgtcg
    21061 tggctgctgg cgttccttgc cgggttattc gcgaaataaa cgaccgggat aagcactatt
    21121 attacaaaga ttataaagtt gaatcgtcag tttaaattat aaaaattgcc tgatgcgctc
    21181 cgcttatcag gcctacaagt tcagtgatct acgtaggccg catccaacgt aaacaaagcg
    21241 caggaacaag cgtcgcatca tgcctctttg acccacagct gcggaaaacg tgctggtgca
    21301 aaacgcaggg ttatgatcat caacccaacg acgcacagcg catgaaacgc ccagtccatc
    21361 aagtaattgc cactgatact acgcagcaca ccagaaaacc acggggcaag cccggcgatg
    21421 ataaaaccga tcccctgcat aaacgccacc agtttgccag caatagcggg ttgcgcagag
    21481 tgatcgagtg ccagcagcaa acagagcgga aatgcgccgc ccagacctaa accacacacc
    21541 atcgcccaga agaccggcaa ttgcagcggc agccagataa agccgcagaa acccaccagt
    21601 tgtaacgcca gcgccagcat caaaagtttg cgccgatcct gatggcgagc catagcaggc
    21661 atcagcaatg cccccgcagc ttgcccaagc gtcatcaacg ccagcaagga accgctgtac
    21721 tgcgcgctgg caccaatctc aatatagaaa gcgggtaacc aggcaatcag gctggcgtaa
    21781 ccaccgttaa tcagaccgaa gtaaacaccc agcgtccacg cgcggggagt aaataccacg
    21841 cgaaccggag tggttgttgt cttgtgagaa gaggcgacct cgcgggcgtt ttgccaccac
    21901 caggcaaaga gcgcaacaac ggcaggcagc gcccaccagg cgagtgtttg ataccaggtt
    21961 tcgctatgtt gaactaacca gggcgtaatg gcggcaccaa gtccaccgcc gcccattaga
    22021 gccgcggacc acagccccat caccagtggc gtacggtgct gaaaccgccg cttaattacc
    22081 gaaggcatca ctgcctgaat gatgccgatc cccactccac caagcagtgc gctgctaagc
    22141 agcagcgcac tttgggggta aagctcacgc atcaatgcac cgacggcaat cagtaacaaa
    22201 ctgatggcga cgctgcgacg ttcgctgaca tgctgatgaa gccagcttcc ggccagcgcc
    22261 agcccgccca tggtaaccac cggcagagcg gtcaacaggg cagccacgct aaagctcatt
    22321 ccgcttgcct ggcgcaattg cggtagcagt ggcccgacgg aggtgagcag tggtcgcata
    22381 ttaagaccaa tcagcaccag aaccagcagc atcgtgaacg tcttttgatg gttacgcatg
    22441 gctgtcagaa cggtttggtc ggcagatatt taccatctaa ggtgatgacc gcacgttcgc
    22501 caccctccgg gtccgccact ttcttaacgt cgagtttgaa gttaatcgcg ctgataatgc
    22561 catcgccaaa cttctcatga accaacgctt tcagggttgt gccgtacacc tgcaacattt
    22621 catagaaacg atacatcgtt gggtcggttg gaatacggtc atcaatgcag ccacgcagtg
    22681 gaatcatctg caacagtaga atggcgtctt cgtcgagatc cagcttcgct ccgaccaggc
    22741 gggcggcgtc ggcaggaagc gcctgctgcc ccagcaaagc cgcggttaca aaggcttctg
    22801 ccagaccggt gccgtcggca atctcggcaa atgagagatc ttttttagct ttgctgagca
    22861 aaatggcatc ggcaagatca agacgaatat tgcggttaat ttgcgactga atcatggtgg
    22921 atctcctgat ggtttaaaaa taaggttacg ctgcggtcgg ttggcgtagc ggtatggcac
    22981 aaacgcgagg attagcggcc agtggcacaa actggcgggt tgcgccgtca aaagctgcga
    23041 tgctgccgct ttcaatgtcg tagacccagc cgtgcagggc aatccgcccc tcttcgagcg
    23101 ccaggcgcac cgatggatga gtttgcaaat tagccaactg agcaatgacg ttttcacgta
    23161 ccatcgccgc tgcttttgac ggtaaatcgg gatgcgggcg cgcctcatta acgacgcggg
    23221 ctgaatcggc ataacgcagc cagtgggaga cggcaggcat atggtccatg cactgacagc
    23281 tggcaatgga ggtcatcgcg ccacagttgg aatgaccgca aatcacaatg tcagataccc
    23341 gaagtgcagc gacggcatac tccaccgaag cagaaacgcc accgggttcc ggcccgtagg
    23401 aagggacgat attgcccgcg ttgcgaataa cgaacagatc gccaggctca cgttgcgtca
    23461 ccagctcagg gaccagacgg ctgtcggagc aggagataaa aagggtgcgc gggctttgct
    23521 gtgtcgccag ctgtttaaac aaggcttccc gcttcggaaa tgcctcgcgc tggaatttaa
    23581 ggaatccatc aataatctct ttcaccgtta acctctgtct gtctctggaa tgagaggcag
    23641 actactcgcc gattgtcata aggtaaaaga ctcatttatg atgagttcta ttggatttac
    23701 ttataggttg gcgaatgctc tctcgacata tcaattactt tcttgccgtg gctgaacatg
    23761 gcagcttcac ccgtgcagcc agtgcgttgc acgtctccca gcctgcgctt tcccagcaga
    23821 ttcgccagtt agaggaaagt ttgggcgtgc cgctgtttga ccgtagcggg cgaacgattc
    23881 gtctcactga tgcaggagaa gtctggcgac agtacgccag ccgggcgtta caggaactgg
    23941 gggcgggtaa acgggcgatt catgatgttg ccgatctgac gcgaggatcg ctgcgtatcg
    24001 ccgtcactcc cacctttaca agctacttta tcggcccttt aatggcggat ttctatgcgc
    24061 gctaccccgg catcacgctc cagctacagg aaatgtcgca ggagaaaatc gaagatctgc
    24121 tttgccgcga cgagttggac gttgggattg ccttcgcgcc tgtgcattcg ccggagctgg
    24181 aggcaattcc tttactgaca gaaagtttag cgttagtcgt ggcgcaacat catccgctgg
    24241 ctgcctgtga acaggtggcg ttgagtcgct tgcatgatga aaaactggtc ctgctcagcg
    24301 cggaatttgc caccagagag caaattgacc actactgcga gaaagcgggg ctacatccac
    24361 aggtggtcat tgaggcgaac tcaattagcg cggttctgga gctgattcgc cgcacttccc
    24421 tttccacatt gttgccagca gcgattgcca cacaacatga cgggcttaaa gctatatccc
    24481 ttgccccgcc actactggag agaacggcgg ttttgttgcg gcggaaaaat agctggcaga
    24541 cagccgccgc gaaggcattt ttgcacatgg cgttggataa atgcgcggtt gttggcggaa
    24601 atgaatcgcg gtaaccactg acacaattgc ctgatgcgct acgcttatca ggcctacgag
    24661 ttcagtgctc tacgtaggcc ggataaggcg ttcacgccgc atccggcata aactacgctc
    24721 gctttgtcaa cacacagccc agccgatcac cgtttgtaat cgatggcttc tggctgctcc
    24781 agatatacgg tggtttgtgc tggttgggtg ctggcaatca ccttaccgcc acgtaccgaa
    24841 taacgtacag gaacctgacg gcgcagcgcg tcaaacccat tttcagccgg caggataatc
    24901 aggttggcgc tgtttccggc ggcaatgccg taatcctgca aattcaacgt cctggcactg
    24961 tggtgggtga ttaaattcag gccatcgtta atctgcccgt agcccatcag ctggcaaaca
    25021 tgcagcccca tatgcagcac ttgcagcata ttcgccgttc ccagcggata ccacggatcg
    25081 aagacatcat cgtgaccaaa gcagacgtta atgccggact ccagcatctc tttaacgcgc
    25141 gtgatgccgc gacgttttgg ataggtatcg aaacgtcctt gcagatggat attgaccagc
    25201 gggttggcga caaagttaat accggacatt ttcagcaagc ggaacaggcg tgaggtatac
    25261 gccccgttat aggagtgcat tgccgtggtg tggctggcgg tgactcgcgc gcccattcct
    25321 tcacggtgcg ccagggcagc aacggtttcg acaaagcgcg actgctcgtc atcgatctca
    25381 tcacagtgaa cgtcgatgag acggtcgtat ttttgcgcca gggcgaaggt tttatgcagc
    25441 gactccacgc cgtattcacg ggtaaattca aaatgcggaa tcgcccccac tacgtcagcc
    25501 cctaagcgta acgcctcttc cagcaacgct tcaccgttgg gatacgacaa aatcccttcc
    25561 tgcgggaagg cgacgatttg cagatcaatc cacggcgcga cttcctgctt cacttccagc
    25621 attgctttca gcgcagtcag cgttgcatcc gaaacatcga catgggtacg cacatgctga
    25681 atgccgttgg caatctgcca tttcagcgtt tgccaggcgc gttgtttcac atcgtcatgg
    25741 gttaataacg ctttgcgctc ggcccagcgt tcaatgcctt caaacagcgt gccggactga
    25801 ttccagttcg gttgtccggc ggtttgcgtg gtgtccaggt gaatatgcgg ctccacaaac
    25861 ggcggtataa ctaaaccttg ttcggcatcc aggctgtttt cagttatggg catcaccccg
    25921 gattgcgcat caatggcgct gatttttccg tccagcagat gaatctgcca cagcccctct
    25981 ttgccgggta accgggcgtt aataattgtc tgtaaagcgt tattcgacac tgttagcctc
    26041 cacatgcgtc attgcagccg tcgttttgcg attcaaaatc gggttaagga tcagatagct
    26101 cagcgcgcca cctaataccg cgttgaccgg aacaataccc ggtaaccagt ggcctgcggc
    26161 aatccccaag gcgaccgcca gaatcgccac ccaattgaca ctcatcatac gcgtggtcgc
    26221 aaagtgctca tagcggcgac ggttcatcag atagtcagcg atgatcacgc cccctaccgg
    26281 aggaatagct gccgaaagga aggtcagcca gccgacaaaa ttgttataca gccataatgc
    26341 gcagaccgta ccgataatac cgttgattac cgaaagggtt ttactcgaca taccagtaat
    26401 gttggcgaaa cctaaacccg acgcatagag cgcgttatcg ttggtggtcc agatattcag
    26461 ccccagcacc acaatcgcag gcagcagcag gccctgagca atcatcacat cagagatatc
    26521 cgccatcccc agtgccgcag cgcccgctgc accgaaaata aacatcaacg agttgccgag
    26581 gaaaaaggcc accatcgcca ccagcaccgc cagtttggca ttacgaccaa accggacaaa
    26641 gtcagcggtg agcgtgcccg cactgataaa tgaccccaca accagcgcca gcgcgacatt
    26701 gaaatctaac ggttgtgcgg gaacgaccgc ttttaatgcg tccaggccgc ccatgccatt
    26761 aacggccagc cacacggaat aaccgccaag gcaggcgata gccggaaccg caatcaccga
    26821 aagaaccgtc agcgccgaaa tgccaaagaa gacggtgacg gtcatcagta aaccggaaac
    26881 ggcaatcagc aaattaatat ccagcccggt tgccttgccc accggaatgg caaacatcgc
    26941 cacgccaacg ccaaaccagc caacctgggt gccgcccagc agcagtgaag gcagccatga
    27001 gcctttaaca ccaaaagaga agcgagcaag aagatgagtg gtcaggccgg tttttgcgcc
    27061 aatgtaacca agaaatgaag tgtaaatacc gaggagaaga ttaccgatga ggactgcgag
    27121 gaagaaatca tgatagctaa gaccggttcc gagagtgccg ccggtccaca tactggcgga
    27181 aaagaaggtt aatcccagca tgacgaacgt caatgccaat acccctttcc gcgccgactg
    27241 cgggactggc ccctggctaa agttgttatc ttgcgacacg aaattccccc tttttgctgt
    27301 ttgaaaccca aaaaatccgc cgcattctat tcatctgaag ataaaaagca atcgttttcg
    27361 taggggaata tatttcttat atgaatgagt acaaaaaggc gggggaaact tatctgtgta
    27421 aaaacagtgg gtaggcatga taagacgcgt cagcgtcgca tcaggcatct gcgca
//
LOCUS       DAZUCC010000045        27405 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZUCC010000045 DAZUCC010000000
VERSION     DAZUCC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.045"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(67..2937)
                     /gene="xdhD"
                     /locus_tag="ACHY09_001697"
     CDS             complement(67..2937)
                     /gene="xdhD"
                     /locus_tag="ACHY09_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFS3884681.1"
                     /translation="MIIHFTLNGTPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEEDNC
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(2934..3713)
                     /gene="ygfM"
                     /locus_tag="ACHY09_001698"
     CDS             complement(2934..3713)
                     /gene="ygfM"
                     /locus_tag="ACHY09_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFS3884682.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQTLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEEYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVEKQNLEGDALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3764..5092)
                     /gene="ssnA"
                     /locus_tag="ACHY09_001699"
     CDS             complement(3764..5092)
                     /gene="ssnA"
                     /locus_tag="ACHY09_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFS3884683.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKGGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDDAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSHSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLCDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(5095..8193)
                     /gene="ygfK"
                     /locus_tag="ACHY09_001700"
     CDS             complement(5095..8193)
                     /gene="ygfK"
                     /locus_tag="ACHY09_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFS3884684.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSCKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPPELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTELKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDNEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(8515..9093)
                     /gene="mocA"
                     /locus_tag="ACHY09_001701"
     CDS             complement(8515..9093)
                     /gene="mocA"
                     /locus_tag="ACHY09_001701"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFS3884685.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKIMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            9197..9967
                     /gene="yqeC"
                     /locus_tag="ACHY09_001702"
     CDS             9197..9967
                     /gene="yqeC"
                     /locus_tag="ACHY09_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFS3884686.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRSECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            10015..11640
                     /gene="yqeB"
                     /locus_tag="ACHY09_001703"
     CDS             10015..11640
                     /gene="yqeB"
                     /locus_tag="ACHY09_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFS3884687.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTECGFIPVIVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(11681..12613)
                     /gene="arcC"
                     /locus_tag="ACHY09_001704"
     CDS             complement(11681..12613)
                     /gene="arcC"
                     /locus_tag="ACHY09_001704"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS3884688.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(12661..14046)
                     /gene="hyuA"
                     /locus_tag="ACHY09_001705"
     CDS             complement(12661..14046)
                     /gene="hyuA"
                     /locus_tag="ACHY09_001705"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFS3884689.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGSNISPQVPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(14099..15310)
                     /gene="ygeY"
                     /locus_tag="ACHY09_001706"
     CDS             complement(14099..15310)
                     /gene="ygeY"
                     /locus_tag="ACHY09_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFS3884690.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(15368..16564)
                     /gene="dpaL"
                     /locus_tag="ACHY09_001707"
     CDS             complement(15368..16564)
                     /gene="dpaL"
                     /locus_tag="ACHY09_001707"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFS3884691.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(16622..17809)
                     /gene="ygeW"
                     /locus_tag="ACHY09_001708"
     CDS             complement(16622..17809)
                     /gene="ygeW"
                     /locus_tag="ACHY09_001708"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFS3884692.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            18288..20066
                     /gene="ygeV"
                     /locus_tag="ACHY09_001709"
     CDS             18288..20066
                     /gene="ygeV"
                     /locus_tag="ACHY09_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS3884693.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFISEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(20106..20585)
                     /gene="xdhC"
                     /locus_tag="ACHY09_001710"
     CDS             complement(20106..20585)
                     /gene="xdhC"
                     /locus_tag="ACHY09_001710"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFS3884694.1"
                     /translation="MNHSETITIECTINGIPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITGIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(20582..21460)
                     /gene="xdhB"
                     /locus_tag="ACHY09_001711"
     CDS             complement(20582..21460)
                     /gene="xdhB"
                     /locus_tag="ACHY09_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFS3884695.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCQLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(21471..23768)
                     /gene="xdhA"
                     /locus_tag="ACHY09_001712"
     CDS             complement(21471..23768)
                     /gene="xdhA"
                     /locus_tag="ACHY09_001712"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFS3884696.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAISINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAAPIHNGGN
                     LLKQSSMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALNIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAKSSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            24184..24936
                     /gene="actS"
                     /locus_tag="ACHY09_001713"
     CDS             24184..24936
                     /gene="actS"
                     /locus_tag="ACHY09_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFS3884697.1"
                     /translation="MSAGRLNKKSLGIVMLLSIGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKNSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            25015..25088
                     /locus_tag="ACHY09_001714"
     tRNA            25015..25088
                     /locus_tag="ACHY09_001714"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25047..25049,aa:Gly,seq:ccc)
     gene            25253..26467
                     /locus_tag="ACHY09_001715"
     CDS             25253..26467
                     /locus_tag="ACHY09_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3884698.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARAARDEAKRLIAGGVNPSDVRKAHKREQASKFGNTFEA
                     IAREWYEKRLDRWSQSYAAEMIDTFEKDVFPYIGSKPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGKCG"
     gene            26595..27290
                     /locus_tag="ACHY09_001716"
     CDS             26595..27290
                     /locus_tag="ACHY09_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884699.1"
                     /translation="MPVVNDFPDLSHWFTVQEFTITQAALLLSGIDPLDYPTLEMVKE
                     EKHERWRMAYGFAYGMVTAIRRGVLTPVLCVAYDWGDMRRLTPAEMMDRSNNISMDGT
                     IITRYSLQEWVKNEKVDYVRPSRSISDKEIKPSNQSVIIDAESSVINSEETVLLTYGH
                     KSEGLEYVEDAIRELWATYDPDNQQTAPTRNDVVEYLKGRGAGQNMAEAVNLILRPTG
                     LQQVGRRKRKTNS"
BASE COUNT         6633 a         6689 c         7121 g         6962 t
ORIGIN      
        1 tttcgcagtc actatttagt catcgttcaa acagagaaac aggccgccga agcggccatt
       61 tattacttat attttttcca gcgcggtgag tattttctcc ggtgtgaaat gccattcgcg
      121 taaccagatg ccgcaggcat cgtgaattgc ggtagcaatc gccggagcgg cgccatttac
      181 accgatttcc gagatcgatt tcgccccgaa tgggccgact ttatcgtcgc tcggcaccag
      241 cacagcgcgg aaatcacgcg gaatgtcacc aattttcggt gcgccgtaac tgcgtaaatc
      301 acgcgttaac gggtgacctt cggcgtcata aatgatctct tcgctcatac tgtggccgat
      361 agctcgcaga gtggcaccgt agatttgtcc caacgctaac tctggattga ccggtgtacc
      421 gcagtccagc aaggcgtaga atttatccag gcggatttca cccgtacgcg tgttgacggc
      481 aacttcagcg aagtttgcgc catacgggaa ggcgaaatca ggcgtgatat aactgccagt
      541 tcccaccagt gaaccaaagc cggtgccggt ttcgccttta tgggctatct ctccgaaact
      601 aacttcgcct ttcttgccgc gtacgacgcc cggcgttgct agttggacat ctgccactgg
      661 ctcacccaac atttgcgcac cgtggaacag aattttctcc cgcagatttt ctgctgccaa
      721 acgcgccgcg ttacccgaga agcaagtacc ggacgaggca tatgcgcctt tatcaaacaa
      781 cgcatgatcg gtatcaccgg agataacatg cacgtcctgc ggtgggcagt gcagcacttc
      841 tgctgccagt ttcgtcacca cagtatccag accagtacca atatccgcac cgccagaatg
      901 aacgataaag gtaccgtctg attccagttt gatcatgcag ttagcctgat cgatatctgg
      961 gatccctgat ttctgcatga taatggcgac gccacgaccg atgtgccagt caccgttttg
     1021 tggttttggt gaagaccatt ggatcatctc gcgaccctga cgcaggatct cttccagtgc
     1081 gcagctggcg gcggaaggaa cggaggtcgg cgctttacct tcaccgattg caccgagaat
     1141 tttcagctct tgcccttcgt gtacccggtt acgttcgata atttccagtt ggtcgatctg
     1201 taactgttca gccagttccg ctaatgccat ggtgatagcg aagttacctt tcggtgcgcc
     1261 ataaccctga taagcaccat ttgggcaaat gttgctgtag taggtggtga cctggaaatc
     1321 gacgttatcg cacggatata acggcagcga cagcgccggt ccgttacatg gtacggtgag
     1381 tgagtggttg ccgtaagggc cagtgttggc gcggaaatcc atcttcactg ctgtcaggcg
     1441 accatctctt ttcgctccca gtttgacggt gactttcgca acgtgacgag aggtgttagc
     1501 aataaactct tcttcacggg tgtagcggaa cagtaccgga cgcccggtca cgcaggttgc
     1561 ccaggcgcac acttcttcca gcaggatgtc ctgtttagac ccaaaaccgc cgccaactcg
     1621 ctctttaatg acatgaactt tatgttgttt catgcccacg aggcgcgcga cctggcggcg
     1681 taagtgccat ggtacctggg tggaggcgtg gatcaccaga cgatcgccgt ccatacgggt
     1741 aaagcagata tgtgtttcag tcgggcactg ctgtgcctgc gttgagttat aggttcgctc
     1801 aatgatcaca tcggcatcgg caaagccttt gtccatatca ccaatatgac catgaatact
     1861 ggcagcgata tttttgcgtg ggcgagaacc gatcgggaag ttgatgatca tatgctcgcc
     1921 gcgctgggct gcatgacagt tatcttcctc cagagtatct ggtgcaccag caacatacac
     1981 caccggttca tcgtgcacga caggcgcatc ttccgccatt gcctcgtcaa tcgacattac
     2041 cggcttaagc acttcatatt caacgtcgat gagcttcagt gcttcgagcg caatgtcttc
     2101 actttcagcg acgaccgcag caacgcgatc gccgacgtga cgcattttct tgccgaacat
     2161 tcggcggtca agcggtgacg gttccggtgc gctctgacca cccggggtat agtaaatatc
     2221 cgggcaattc aggtgagtaa taacgtgaac gacgcccggt aaggcttcag ctttgctgac
     2281 atccagatga gtaatcagtg cgtgagcgtg tgggctacgt aacattttaa tgacgcaggc
     2341 gtcagccgtt acgcgatctt caacatagca gggtttcgcc tgcaccattt tcgcggcatc
     2401 agttttagga taatgcttgc caatgacttc aaggtcgtca cggaaagtcg gagcgatatc
     2461 aatggtggcc tgcggattat ttttacgtgc aaccgccagt tcaatgacct gatagtattg
     2521 ctgccagcca gcatcgcggc taaacagacc tgagagcgcg tcgtcgatct cttcgcgggt
     2581 aggtgcgtcg atgcgatcga gaagatcggt gataatcaga gccgcagctg gatcgttata
     2641 accagactgc accacgccaa catcaaccat ggcctgttga accagactta actcattcca
     2701 tttgcccaga gattctgcgg tacgaatatc tgccttctct aactgtgcgg cgataagcaa
     2761 ggacgcgtta acgatattac cgttaaagat aattgcgtca gaaccggcaa acccaaagcc
     2821 atcatcgctg ttgcgtacag agtgcattcc catgttaaac aacagctttt gcacgttttc
     2881 accaggatta acggttagct cctggggcgt gccatttaaa gtaaagtgga tgatcatacg
     2941 gcttcctccc cagcctgttg gcagtcggca taaaggtcgg ctaccagaac tcccgtgata
     3001 tagcgtttat aggccacgct gccccgcaaa tcttcctgcg ggaaaatggc gttggcgaca
     3061 gcctgttcaa gggcatcacc ttccaggttt tgtttttcaa catcgtgcag acgcagtgct
     3121 ttactggcta cgccatccag cgcaattcgc ataccgtcgt ggtctgttat tgcaacggct
     3181 gccgtcacga cggttaaacc tgcctgagaa cggctaattt tgcgagtcgc acaggtgcga
     3241 tacggatctt taatgatgat ttcggttagc aggcgatcgc atgggcaggc caggtactcc
     3301 tcaattgaca gagtttcgcc gttgccaaaa accagttcag catccagtgc cagcaggacg
     3361 ggaagcagca ctgactcttc ttgccgggcg gcaatttcac caccaatggt cgactgatta
     3421 cgaacatggc gtgagtaaac aaaaccgaga gcttcacgca gtgctgcagg aataaatcgc
     3481 gcatcacgca gtgtttgcaa gcgagacatc gcgccaatcc gcagtgcacc gttatcccag
     3541 tcaacccagt ccagttccag atcctgtaag gaaatggcaa tctttttatc ggtacgggtt
     3601 ggtgtagcgt tgagtttgct gcccccggcg aaccagacgg cttcatcctg gtagcggcgc
     3661 ttcagttcca gcgcctgttc gacagagtcg ggtctgaaaa attgttcaat catcgtcttt
     3721 ccttaatttc ccctcttctc tgaaagaaga ggggagctgt tatttatgcc agcgcatcca
     3781 tccgacgcca catactggca gcggcttttc tggcttgcgc ataaatggaa tcgcaatcga
     3841 agttaaactg acggtcttca tagaccatca caccattgac catcacgctg tgaacgctgc
     3901 ctgagcccat accgaaagcg atatgcccgg cgatattgtc tgccagcagc ggcgtcggcg
     3961 agttgtaatc gcaaatggtt aaatcggctt tgtaaccggc ttccagaagg ccaaatttcg
     4021 cgccaaagtt gcggctcatc agttcgttgc cgttagccag agctttggca aagctgtcag
     4081 gccacagcgg accacccgca tcgcgatgtt taaagaaggc aaatttcatc tcttcaaaca
     4141 tatctgaacc aataccgtcc gtacccaacg ccagattgcg gatgtcgcaa agatgatggt
     4201 tgtagccgac atggttgttc atgtttgaac gggcgttatg caccaggaat gcatcacgct
     4261 gattgagcaa cgcgatgtcg tctttagaca gataaagccc atgagcgacc agcgttttgc
     4321 tgtcgatgag atcgaattgc gctaatcgtg ccagcagatc tttgccgtac cagtgatgac
     4381 tatgggaaac atcgtaaagg tcttccgcag cgtgaatatg taaaccacgg cctgtggctt
     4441 tcacggcctc acgcagcatt tccagaccgg catccggcac agtaaacggc gcgtgagcac
     4501 caatatgagc ctctaccaga tacggttcag ttgccgcttt cttcgcatca tcaatctgac
     4561 gggcgaagcg gatgttttct tctacacctt cctgcaactc tttgataccg ttgttacggt
     4621 cagtagtttc aaaacaggtc atcgcgcgca ggccaacttt taaaaatgcg ttgcgcaatg
     4681 tggagagcga cccgtcgata tacgccggag aggcatggtg atcgataacc gatgtacatc
     4741 cgcccttaat cgcttccagg gaacaaatca gtccgctgta atagagcgac tcttcatcaa
     4801 gggcgcgatc gagtcgccac cagagatttt tcagcgttga gatgaaatcc gggcaggggg
     4861 cgatgtttgc cataattccg cgggacagcc ccgagtaaaa atggttgtgc gagcagacaa
     4921 ttcctggcat cacaatccga ccatgcatct ctttgtagct ggcatcgggg tagcgttgcg
     4981 tcagggcatc gccgatagcg acaatcacat cattttcgat ggcgatatca acgccttcct
     5041 gcactttcgc tgggtgtaac tgcactgcgg tgacattctt cagaatcaac atgattacac
     5101 ctccacacgg cccagcagat aatgatgatg ctggtggaca tggctaatga tgcggcacat
     5161 atcgttcagc tccggtggta cgttgttgaa ctgaccttcg ttgtcgatgt ttaacaccca
     5221 gctttggtta ttcagacgta cgcgtacccg gcaatcttcc acaaggaagc ctgggttgct
     5281 gctgttatca aagtcttgcg acaggctgaa gacggtgatt ttgtctttgt acggtttacc
     5341 gttccacggg cagaattggg cgcagttgcc acattcgtta cagtaagcgt cgaggtgcag
     5401 cgtctggaaa cggttctgga agcctgggac cgcaatggat acgttggcgc ggttcgggca
     5461 gacatccaca cacttgctgc aaacgtagtt acattcaagg cagcgtgcgg cttcctgggc
     5521 gacaaacgcg tcacgatcgt cgctatccac cagggtgaca gagatatcgc ctttgcgttg
     5581 atagatttcc gccggattaa cgttgttcca gtatttatcg ttctggtggg aacggatatt
     5641 ttcccgacta aggatggcat cggtcgcccg acgcgcggtt ccgacagcag cgacaatgga
     5701 ggatggtccg cgctgtacgt cgccgatcat aaagacgtca gtcagacgag tttcgccatt
     5761 atggtcgacg tctggccagc cgtttttgtc cagcggcacg cccatcgcat tcagggcttc
     5821 agtatcctgc tgttcaccaa tggcggtgat caggctgtct acatgcagtg ttactgtttc
     5881 gttggtttca accggacggc gacgaccttt ctcatcaggt tcgccaagcg acataacgcg
     5941 caaggttaag gtgccatcag catcgaaacg ttccggatta ttcagaaaac ggaactccac
     6001 gccgtcgtgc aacgcttctt catactcttc gcgccatgct ggcatctctt gcagtgaacg
     6061 acggtaaacg accgttgctt tttctacgcc tggaacgcgt aacgccgcac gagcgcagtc
     6121 cattgcggtg ttacccgccc cgacaacgac cacatgtttg cccagcttga gctctgtgcc
     6181 cttgttgtat tcacgcagga aggggagtga tttccagaca ttttggttgt cgcccgccag
     6241 tttcacaccg ctatttttat cagtgccggt ggcaatcaga acatagtgga agccctgatt
     6301 ttttaactgt tcaacggtca gatcgggtga gcagccatac tcaaatttca cgccgtgagc
     6361 ggcaacaaaa tcgatatcgt gctgaattaa ctctggagga atacggaact gaggaatgat
     6421 atttttcacc acgccgcccg cgttggcttc gcgttcaaac agcgtaaccg gatggcccgc
     6481 tctggcaagg aagtaacctg cggccagacc cgccggacct gcaccaatca cggcaaccgg
     6541 atggcgtgaa ccagaaccgg ctggtttgtg ccagcgttgc ttatattcat cccaaccttt
     6601 ttccagcgcg acttttttca gttcgcggat attcagcgca ctgtcgtaat ccaggcgggt
     6661 acagttgtat tggcactggt gatcgcaaat atgaccggta atggcgggca gggcgttgcg
     6721 ttgatagatg agttccagcg cgtcggcata gcggtgttcg ccaagcagac ggatgtattc
     6781 cggaatatct tgcttgatag cgcaggcagt aacacagggg gcaacgtagc aatcggtcag
     6841 cggcaggtct tcagccactt caatacgctc ttctggcttc cagtgtttct gggtgtattc
     6901 catggttaac gcgtctgctg ccagtctgtt cagtcgttcg acgtcaacgt ggtcaagtcc
     6961 ccaggcgtcg gagccttcca gctcgcgcat gcaggcactt aagcgcagat agccgccagg
     7021 tttcagcagg tcggttgcca tagtaatagg gcgaatacca gtatcaaaaa tatcgcggat
     7081 agtcagctga ctggcaccac cggaataaga aatgggcagt ttgccgtcaa aggcgcgaga
     7141 gagaactgct gcaacgttga tggagagcgg gaacagcgca cggcctgaca tatacatctc
     7201 ttcaccgggc agtgcgcctt tgttattaat ggtgccgaga gtgttagtca gttttacgcc
     7261 aaagccgagt gatttttctt ttgccagtgc catcaggcgt tccagcattt ccagtgcttg
     7321 cgtcagcttg aggtcgtgat caaatgactc ttcttttaag ccgatgtaac cgaaaccgca
     7381 gacatcgagg atctcacgaa cacgcgcgta ccccagtaag gtcgggttga gtttcacaaa
     7441 ggtgttgagc cctttttctt ccagcatgta gcggcaaatg gcttcgattt catgcggagg
     7501 acagccgtgc atggtggaga gggtaacccc ttgtaccatg ctggtgggga tgcgagcggg
     7561 taaggcttgc aagctttcgc gtttttcctg taagccgtgg cgggctaaaa atgcgtcatc
     7621 ctggagtagc ttattaagtg catcgcggta ttgagcgaat ttcggatggt cagatgcgtc
     7681 catcatattg tcgatgaact gctgcatcgg cggttgctta ataccttcga ggttgtaacc
     7741 gacgctcata ttaaagatga acgatttaca ggaatcagaa ggctggaaca tcgcttcgag
     7801 aaggtgcagg gcaaaccagg ctttgaggta ttcatcccag gctttaagca gggtaaattc
     7861 ggtagaccat tcggtgttaa agcactcgtc ttcggcatcg atacagggct tttccagctc
     7921 caggcggtca agaatttgga cggtttttag ttcgatgaag cgtccgccag tcagccagga
     7981 agtgacaata ttttgcgcga gctgcgtgtg cgggccagcg gcagggccga cgggagtggc
     8041 acaggtttca ccgaagacgc taacagtttt accttttacg ggtgagtaaa actgttgctc
     8101 gggaatacca aagattgagc gttgttgttg gtattcatca aatatgcgcg tcaaaagttc
     8161 ctcaaacgga atgggacgca taatatcccc cataaccctc tccttagctt atacagatat
     8221 cattgagtga ccggtgttga gaaatccggt ggtttagggg gatagagcaa caatcgcacc
     8281 actcttgcca ggtttggcgg gaaatataaa aaaatgtgag gcggctcgaa acttaatggc
     8341 tttttgtgaa tgaactcaca ggtgccaaat gagaattaaa atcactcctg aaaattatta
     8401 ttgccagccc atttatcact ttttctcttt tctcaaaatc agtgattcct tatcattatg
     8461 ataaagagtg ctggagggat taaatatttg aacgcaatcg tttaactacg ttttctaaat
     8521 ttcagtatac cttttcttcg ctgtaataaa gtcgtcagga gtatcaatat ctaaaattat
     8581 ttctgcattt tctatttcga cggaataatg ttctcccata aggagtgcct gacgcatatt
     8641 ggtgacattg ggtcgctgga ttgcctgcat caggcatggt tttgacacta aaatcggatg
     8701 gccggggatg ccattatgga gtggcagtat tgcgccatca tttcgtaacg accagatttt
     8761 tctaaaaata tcgatggtga gggttggcat atctccgtgg gtgagaaaac aatgttctgt
     8821 ttgcaccgcg ggtactgcgg cctttactga tgtcagtaaa ccctgcgcat aatctgggtt
     8881 gtggataata gtgatattgc tctggttcgc ataacgttcg tgcagttcat taccacgata
     8941 gccggtgact aaaataattc ggctacaaaa ctgcaacgca tttttgatac ttgtatcaag
     9001 aattgttccc tgttgccagg gtaacataat tttccattgc cccattcttg atgataatcc
     9061 tgcggcggta attatacagt cgatggctga catagtgatt ccattattta caggagctaa
     9121 taacgcctga taattgcgtt acttcctgat ggattaagtt gagggtaatt tatcccagag
     9181 aggtccataa agacgcgtga aaagtataat tgacccatcg gcattattca ttgatttagg
     9241 cgcgcagaaa cgtcccactg tgatttctgt tgttggtgct ggggggaaaa ccagcttgct
     9301 tttttggctg gcagaactgt tccaggcgag cggcaggcgt gtattaatca ctacgacgac
     9361 acatatgttt atgccaacat ctcactggcc cgtggttttc tgtcgtgatc ccgccatgct
     9421 tcctcatgcg tcttttatat cccccatttc attttgtttt cacagctgga aagcgaacca
     9481 gggaaaagtg cagggattta cgccagaagc gattgatgca ctggtacaac gatcagagtg
     9541 tgacgtaatt ctcattgagg cagatggctc gcgtggaatg ccgttaaaag cgcctgatga
     9601 gcacgaacct tgcataccta aaagcagttg ctgcgtgatt gctgtgatgg gaggacatat
     9661 tttgggtgcg aaagtgagca cagaaaatgt ccatcgctgg tcgcagtttg ctgatattac
     9721 cgggttaaca cctgatgcaa ccttgcaact gagcgatctc gttgcgctgg ttcgccaccc
     9781 tcagggggcg tttaaaaacg taccgcaagg ttgtcggcga gtctggttca ttaaccgttt
     9841 ttctcaatgt gagaatgcga ttgcgcaaag cgagctcctt caaccgctgc aacaacacga
     9901 cgtagaggca atctggctgg gcgatataca agaacatcct gcaatcgcgc gcagatttgt
     9961 gaattagcgg cccgggtatt ccgggttact gaaaggatat ccattgaggt tggtatgaat
    10021 attttcacag aggctgcaaa actcgaagag caaaattgtc cgtttgcgat ggcgcaaatt
    10081 gttgatagcc gaggctcaac cccccgccat tctgcacaaa tgttagtgcg cgccgatggc
    10141 tctatcgtcg gtacaattgg tggcggaatg gttgagcgga aggtgattga agagtcgctt
    10201 caggcattgc aggaacgtaa gccgcgatta ttccatggac gtatggctcg taacggtgcg
    10261 gatgctgtcg ggtcagattg tggaggtgca atgtcagtat ttatcagcgt ccatggtatg
    10321 cgtccacgtc tggtgttgat tggcgcgggg catgtcaacc gggcgatagc ccagagtgcg
    10381 gcgctattag gatttgatat cgccgttgcc gatatttatc gcgaaagcct caatcctgaa
    10441 ctattcccac catcaaccac gcttctccat gctgagtcgt ttggtgcggc agtggaagca
    10501 ctggatattc gccctgataa ttttgtcctg attgccacga ataatcagga tcgtgaagcc
    10561 ctcgacaaac tcattgaaca acccattgca tggctggggt tgctggcaag tcgccgcaag
    10621 gttcagcttt tcctgcgcca attgcgtgag aaaggcgtgg ctgaagaaca tattgcccgt
    10681 ttacatgcgc ccgttggtta caacataggt gcggaaacgc cgcaggagat cgccatcagc
    10741 gtgctggcag aaatattaca ggtgaaaaat aacgcgccgg gtgggctgat gatgaagcct
    10801 tctcatcctt ccggacacca gctggtggtg attcgtggtg cgggggatat cgccagtggt
    10861 gtagcgctac gtctgtatca tgcgggtttt aaagtgatca tgctggaagt ggaaaaaccg
    10921 acagtgattc gttgtaccgt ggcatttgcc caggccgtgt tcgatggcga aatgacggtc
    10981 gaaggcgtca ccgcccgcct ggcaaccagc tctgcggagg cgatgaaact taccgaatgc
    11041 ggattcatcc ctgtgatagt agatcccgcc tgttcattgc ttgatgaact gaaaccgctt
    11101 tgcgtggtgg acgctattct ggcgaaacag aatttgggga cgcgggcaga tatggcacca
    11161 gtaacaatcg cgcttgggcc gggctttact gcagggaagg attgtcatgc ggtaattgaa
    11221 acaaatcgcg ggcactggct cggtcaggtg atttactctg gttgtgcgca ggagaatacc
    11281 ggtgttcctg gcaatattat ggggcatacc acccgacggg tgatccgtgc tcctgctgca
    11341 ggcattatgc gatccaacgt gaaattaggc gatctggtga aagagggcga tgtgattgcc
    11401 tggattggtg agcatgaaat taaagcaccg ttgacgggga tggtgcgtgg cttgttgaac
    11461 gacggactgg cagtggtcgg tggttttaaa attggtgata tcgatcctcg tggtgaaacg
    11521 gctgatttca ccagcgtttc tgataaagcc cgggcgattg gcggcggcgt acttgaggcg
    11581 ttaatgatgt tgatgcatca gggcgttaaa gcgacgaaag aagtgctgga agtggcttaa
    11641 atgaaaatgg ccggatggta ttacccaccc ggcctttaaa ttactgacaa atcaccgttc
    11701 cggttttgcc ttcaatccct tctttcgctt tgctcaatac ggtaatcagt gcttcacgac
    11761 ctgcgcgaga gcgagcaaac gaggcggcag cttccacttt aggcagcata gaacctttcg
    11821 caaaatgacc ttcttcgata aagcgttcag catcacttaa tgacaggcga tcgagccact
    11881 gttcattctc tttaccgaag ttaatggcca ctttttctac cgcagtcaga atgatcagca
    11941 tatcggcatc aatcatttct gctaaacggg cgctggccca gtctttatcg ataaccgcac
    12001 tggcaccgcg cagatggttc ccttcacgaa taacgggaat gccaccaccg ccaacggtga
    12061 tcactacctg gcctgcttct accagcgctt ttaccgtctc tttttcaata atatcaaccg
    12121 gtttcggcga ggcaacaaca cggcgatagc cgcgaccagc atcttctttc agagtgtaac
    12181 cctgttttgt cagttgctct gcttcctgct cggtaaagaa cgaaccgatc ggcttggtcg
    12241 ggttgaggaa tgccgggtcg ttagcatcga cttcgacctg ggtaaccagc gttgctacag
    12301 gtttattaat gccacgagaa agcagttctt ctcttaatgc gttttgtaga tcgtaaccaa
    12361 tataaccctg gcttaaggca acacagacag acatcggcag cattggcgag tgcgcttcag
    12421 tttttgcggc agcttcaaag gcctggttaa tcatgcccac ctgtggcccg ttaccgtgag
    12481 tgacgataac ttcatgtccc tgggcaatta aatcaacaat tgcctgagaa gtaattttta
    12541 ccgctttcat ttgtccggcc aggtcatcgc ccagcgcatt cccgcccagg gcgagaacaa
    12601 ttttcttact catttttcct cactcatcat tcttgattgt gtatagatcc ctggcagtgg
    12661 ttagagcacg ggagggacaa acggtttgcg tcgcaggaaa cgacctcgcc cggctttgcc
    12721 tgtaaaggtg ccgtcacaga aaatcgtttc accacgggat aaagttctga caatcgcgcc
    12781 ctgacaggta aaaccctccc atggcgagta gtcggcgttg tcgtggagat ggcgatgctg
    12841 aatttgttga ctctgacgtg ggtcgataat caccacgtcg ccatcggaac cgggcgctaa
    12901 taatcctttt tgcggccaca ggccaaacag cctggcgggc atcgcgctgg ttaattcaac
    12961 aaaacgttcc ggtgttatac gtcccgtcat aacgccactg gaaaacagta actgcatgcg
    13021 gttttccaca ccgggtaagc catttgggca gcgactgaaa tcgcctttag aaatttgcag
    13081 gcgttgagcc atcgagaatg tgcagtgatc ggtcgctacc acgtcaatcg caccatcgct
    13141 gatgccacac cacagtttgt cctgctcgcg tacgttacgc agcggtgggc taagaatgaa
    13201 cttcatgcca tcttctgtat cgtaactgcg ttcgtccaac aggagatatt gtgggcaggt
    13261 ttcaacccag actggctggt gattcgcacg ggcaagacgc agataatcca gacctaagcc
    13321 gttagacagg tgcacgatat agagcggggc gttaccggca atttgtgcca ggttaatcat
    13381 gcgggcgatg gcttccgctt cgcattccag aggtcgactc aaggcatgat aacgcggcgc
    13441 ggttaaccct gcggcgataa actccgcccg cttgctggcg atagctgcgt cattttccgg
    13501 gtgcacggtg gtcagcgcgc cggattcatg caagcggcgt aatgcctgca acacttcatc
    13561 atcgttgagt ttatattgat aggttaaata gagtttaaaa ctgctcagtc cttcctcaac
    13621 catcatcgga atttcgtcga ggatagcgtg attgatatgt tgaatgacac cgtgaaagct
    13681 gtagtcgatg actgctttgt gggcggcata accgcgataa acttccagtt gatggcgtaa
    13741 ccggcagcca ttcgggccaa atcccatatg gtcaataatg gttgttgtac cgccacacgc
    13801 agctgcgcgg gtaccggtaa aaaaatcatc acaactgcgc gcgatgccga catcaatatt
    13861 gaaatgcgta tggacatcca cgccgccagg gaaaacgtaa cagccagtgg catcaatttc
    13921 ttcatacggg acctgcggcg aaatattgct gcccaattgg cgaacaatcc cgctctcaat
    13981 cagcaaatct tgtttggctt gtccatctgc gttaacgaca gtgccgtttt tgatcaatac
    14041 gcgcatagca aactccaaaa accctccggc gaaccggagg ggaataagtg aagagtaatt
    14101 attcggttgc cagccagctt aacgggattg cagcgtacat cgcggcacag gtcaccaggt
    14161 gagatttcca ggttttttcg ttaggtgcat gcgcttcagg ttctttaccc gggccaaagc
    14221 cgatcaccgg aatgccgtga cggcccatga tagatacgcc gttagttgag aaggtccact
    14281 tatcaacaac cggcgctttg ccaaacagac cttcgtaggc attcaccagt gctttaacgg
    14341 tgaagtgatc ttcttccact ttccaggtcg ggaagtagca ttcggttggg taaaccaggc
    14401 cagtccagga cggacggtcg tagttgtaca tagaaacaac cgcgttagct ttctgtactg
    14461 caggcagggc gcggatttcg tccagcgcgc cttcccaggt ttcgccccag gtcagacggc
    14521 ggtcaataga gacggcgcag ctatctgcta cagcgcaacg gcttggggat gtgaagaaga
    14581 tttcagaaac ggtgagggtg cctttgccga ggaattcgtc ataacccaga cgttgggaga
    14641 gttcttgtaa ttcgccaaga atcggaccca ttttgaaaat ggcgttgtca ccgcgttctg
    14701 gtgcagaacc gtggcagctc acaccctgaa catcaatacg aatttccata cgaccgcgct
    14761 gaccacggta tacctggcag tcggttggtt cggtactgac cacaaattcc gggcgaatgc
    14821 cggattgttc aataatgtac tgccagcaca ggccgtcgca gtcttcttcc tgcacagtac
    14881 cggtaaccag cagggtatat tcatcttcca gaccgaggtc tttaatgatt ttaccggcat
    14941 aaaccataga tgccatgccg ccttcctggt cggaagtacc gcgaccaccg atcagctcat
    15001 cagtttccat gccttcgtac ggatcgaagt cccagttttt gatgttgcca atgccgacgg
    15061 tatcgatatg agcgtccatt gccaccagac gcgggccgtg gccgatataa ccgagaacgt
    15121 tgcccatcgg gtcgatttca actttatcga agccgacttt ttccatctct tctttaatac
    15181 gatgtactac gcgtttctcg tcgcagcttt cactgggaat agcaaccata tcgcgcagga
    15241 agcgagtcat gtctgcctgg taatcttttg ctttttcaag aatcagtttg aatggaatat
    15301 tcttagccat tatttttctc cagatttttt actccccgtt ttgcagggag cagttccaat
    15361 agggtgatta aggtgctaca gcgtgtttgc cttcccagac aacttcgcgg tagtgcttca
    15421 cgtcggtgtc gccttcagtg ctgataacca gcaccacggc atctttgttc agcgccagtt
    15481 tttccatcag gctttgacgt tgcgggtgat aatgaaccgc tgcgagaacg cccaaaccga
    15541 cagcgccgga ttcaccggag atgatgcgcg ggtcgttgcc atacggatta cccagcacgc
    15601 gcatacctaa tgcggcaacg ctgtcctggc aggagatgaa ttgggtggcg cagttacgta
    15661 ggatttccca gcccagtggg ttaggttcgc cgcaggccag gcccgccatg atggtggcca
    15721 tatcaccgcc aacgttgacg atgtcgcctt tgacgccgga gcgataaata cagtcagctt
    15781 tgtcaggttc aacaataatg ctgtgcagat tttgcgggct atagacgtcg accagataac
    15841 ccagcacacc accggccatt gcaccgacac cggcttgcag cagaacgtgt gtcggggtga
    15901 cgcccatttc acgcatttgc tcgacggctt catccgccag tgttgcgtag ccttgcatga
    15961 tccaggttgg gattttggtg taaccttccc atgccgtgtc ctgtaccact tcccagccgt
    16021 gctgctgcgc gtgttgcatg gtcaggcgaa cggtatcgtc atagttcata tccgtgacga
    16081 tgcactcggc accgaggttt agaatggcgt caacgcgttc ctgagcagaa cctttcggca
    16141 tgtaaatcac cgcattctgt ccgagttgct gtgctgccca tgccacaccg cgcccgtggt
    16201 tgccgtcggt ggtagtcgcg aaagtcattt tttcgccgat ggcatttttc aggtgctcaa
    16261 atgacagcgt ttcgatatca agatgatatt tttcacacaa taattgagcg atggcgtacg
    16321 caccgccaag cattttgaag gcgttcagac cgaatcgttt tgattcgtct ttgacgagaa
    16381 ttttctttac accaaacagg tttgcgagat cgtccagcgc acaaagcggt gttgggtgat
    16441 aaccagcaat tttctggtgg aactggcgtg ccagttttgc ctggctttgc gaaaaaagcg
    16501 gtgatgtttc gccgttgaaa aatttgttat cggcgatatc aatcttcaat gagaaaacgg
    16561 acatatccta tccttttggc ctctggaaat agagggccga ccagctgatc ggcccgaggg
    16621 gttatttcac gcgttcttgc gcttctttca gcagttgctc gagcagtgca cctggtttgg
    16681 cgtatttacg ggacaggatc atcgcggcga tgatataagg cttccagctg gcttctttgt
    16741 aggtagcgat acggtatttt tcgaatacgc cttcagtcac ttcaccttct ttgcaggata
    16801 cgccgctgat atcagctggc aggcagtgca tgtacagggc ttcgccatca cgggtcagtt
    16861 ccatcatctc ttcagtacaa tgccagtctt tgtgttgcgc gttctgtgcc agacactgtt
    16921 tttccagtgc ttttaagcct tcgtgatcgt tcgcacgcag caattcagta cgctcttcca
    16981 tcactttgta aggtgcccat gacttcggat aaacgatgtc tgcgtctttg aaggcttctt
    17041 ccatgctggt gacctgacgg aagctaccac cggaggcttt agcattgttt ttagcaactt
    17101 caacgacatc cgggatcagg tcgtagcctt ccggatgggc cagggtgaca tccataccga
    17161 agcgggtcat cagaccgatg atgccttgtg gtacagagag cggtttgcca tagcttgggg
    17221 agtaggccca ggtcatggcg atttttttgc ctttcaggtt ttccagcgaa ccaaagtgtt
    17281 cacgcaacca tgccaggtca gccatagact gagtcgggtg gtcaatatcg cattgcaggt
    17341 tcactaaagc cggacgctgt ggcagaaccc cttgtttgta gccgtcatca agtgccgcgc
    17401 caacttcacg catataggcg ttgcctgcgc ccagatacat atcgtcgcga ataccgatgg
    17461 catcggcgca gaaggagatc atattggcgg tttcacgcac ggtttcgccg tgagcgattt
    17521 gtgatttgcc ttcgtcgaga tcttgctgtg caaggccgag caggttaagc gcggaagcat
    17581 aagagaagcg ggtacgggtg gagttgtcgc ggaatacgga aatacctaat ccactattaa
    17641 agactttggt tgagatgttt tcagcacgca gtgcttttaa tgctgcggca acgtccagta
    17701 cttgtttcag ttcatctggc gtctgttccc acgttaacaa aaaatctttc tcgtgaaggt
    17761 gagaggtcag cgaattgata tccttaatca gctcattaac agttttcatc ataaagtccc
    17821 ggtagtggaa tgacaggcaa agtggtttat ttcactgtga cttgccaacg tgattggtgt
    17881 tttcacagct atacctggga ctaacaataa gtgtgccaga attatccacg gggctgcaaa
    17941 agactaattt gacggataat gtgtggggtt gatcacaaat tgatgccagc ttctggttgt
    18001 ttgtaaaata cgaaaacgtt tggctttttt tatcgcgtta tttatttgct gctaagtatc
    18061 agaaataaga taaagggaca ggcgatctct cttcagtttg ataaactcac cgccgtaatt
    18121 ctcatattga tatagaaatg tgatatctct tccataattt cattagaaag tgataaaaat
    18181 cttatgtttt tatcaaagcc aatttcgcct gagttaatat catcatgatc tatgaaccat
    18241 caacgtcttc aatgatcgac ttttaagtaa gtctggaagg taaagctatg gagcttgcta
    18301 ctacgcagtc agtattgatg caaattcaac cgacaattca gcgttttgcc agaatgcttg
    18361 ccagcgtttt gcagcttgag gttgagatcg ttgatgaaaa cttgtgtcgc gtcgccggaa
    18421 cgggcgcgta tgggaagttt cttggtcgcc agttgagcgg caactcacgc ctgctccgcc
    18481 acgtcctgga aacgaaaact gaaaaagttg tgacacagtc tcgcttcgat cccctttgtg
    18541 aaggttgcga tagtaaagaa aattgccgcg aaaaagcatt tctgggtacg cctgtcattt
    18601 tacaggatcg ttgtgttggg gtgataagtt tgattgccgt tacccacgag caacaagaac
    18661 atatcagtga taatttacgc gaattttctg attacgttcg ccatatatcc accatttttg
    18721 tttcgaaact tctggaggat caggggccag gagataacat cagtaaaata ttcgcgacca
    18781 tgatcgataa tatggatcag ggcgttttag ttgttgatga cgaaaatcgg gttcagtttg
    18841 ttaatcagac tgccttaaaa acactcggtg ttgtgcaaaa taatattatt gggaaacctg
    18901 ttcgtttcag accattaaca tttgagagta attttaccca tggacatatg cagcatattg
    18961 tttcgtggga cgataaaagt gaattaatca ttggtcaatt gcataacatt cagggccgac
    19021 aattattttt aatggcgttt caccaatcgc ataccagttt ttctgtagca aatgcacctg
    19081 atgaaccgca tattgaacaa ttggttggcg agtgccgtgt tatgcggcaa ttaaaacgac
    19141 tcattagccg tattgcaccc agcccatcca gcgttatggt ggttggcgaa agcggcacgg
    19201 gtaaagaagt cgtcgcccgt gcaatccata agttgagcgg aagacggaat aaacctttta
    19261 ttgctatcaa ctgtgccgcg attccggagc agcttctgga gagcgaactg ttcggttatg
    19321 ttaaaggcgc atttactggc gcttctgcca acggtaaaac agggttgatt caggcggcga
    19381 atacgggcac gctgtttctc gatgaaatag gtgatatgcc attaatgttg caggctaaat
    19441 tactgcgcgc tattgaggca cgtgaaattc tgccgattgg tgccagtagc ccaatacaag
    19501 tcgacattcg catcatttct gcaactaacc agaatttggc ccagttcatt tccgaaggta
    19561 aattccgcga agatctcttc taccgactta atgttatccc gataactctg ccaccgctgc
    19621 gtgaacgtca ggaagatatt gaactactgg tgcattactt tttacatctg catacccgtc
    19681 gtctgggatc ggtttatccg ggcattgctc ccgatgtcgt cgaaatattg cgtaagcatc
    19741 gttggcccgg aaacctgcgc gagttaagca atttgatgga atatctggtt aacgtggttc
    19801 cttcaggtga agttatcgac agcacgctat tgccgccaaa tctgctgaat aatggcacaa
    19861 cggagcaaag tgatgtaaca gaggtcagtg aggcgcacct ggcactcgat gatgcgggcg
    19921 gcacggcgct ggaggagatg gaaaagcaaa tgatccgcga ggcgctttca cgtcataaca
    19981 gcaagaagga agttgctgat gaactgggca tcggcattgc tacgctctat cgcaagatta
    20041 agaaatatga gttgttaaac acataacctc agtgaatccg gcctgaattc aggccggata
    20101 tccttttact tcgttttctc gcaatccaga actgtattta caatcatctg ataccccgtg
    20161 cagcgacaaa gatttcccgc cagtccacga cgaattcccg taatggttaa tggcttctcg
    20221 cgtggtttcg ccagcattgc cgtggtagcc ataatcaggc ccggcgtaca aaacccgcac
    20281 tgcactgcgc cggatttcgc ataagcctgc tgaacatgag aaagttttcc gcctttcgct
    20341 tcaccttcca gcgtgcggat ctcttttcct tcagcccagg cggcaaggta taagcaactg
    20401 tctattgctg tgccgtcgac cagcaccgta caggcaccac attcacccac gcagcaccct
    20461 tgtttgacac ttagcagtcc ttgttcgcgg agtaattccg agagcggcgt gcctggtgcg
    20521 gcgtgaagct gaaaaggtat cccgttaatg gtgcattcga tggtaattgt ttcgctgtga
    20581 ttcattgcaa ttttcccccc gccgcggcga cggcttcgct aatcactttt ttggtcatcg
    20641 tctggatgag atgcagacga aactctttac tggcccgcca tgacgaacgc ggggcgacat
    20701 cttgcaggac tgattcgctg atggcttcca gcgtttgcag gtttaatggc gcattttgtg
    20761 cagtctgttc ggcatgttgg cagcgaattg gcgttggcgc ggcaacacca aatgccaggc
    20821 gtaattcgct gaaattgccg ttatccagtt ggcaatgtgc ggcgcagcca atcgttgaaa
    20881 tatccattgc gtcgcgcatg gcatatttaa aatgcgcgct gcccgcgtgt tctttcggct
    20941 ggggcggaaa atgaaaggcg acaaggattt cgtcatgctc aagagacact ttgcccggcc
    21001 cggtgtgaaa gccattaatc gggacgaaac gaacaccgcg cggggagtgg atctccagtt
    21061 tcgcgtcata aattagcgtt ggcgtggcag aatctgcgct ggtggcaccg ttgcaaatat
    21121 ttccaccgta ggtagcgacg ttacggatct gcggtccggc aatggatgag gccgcagcac
    21181 ataacgccgg gagatgacgt tgagttacag gatcttcaat gagctgggta aatgtcgttg
    21241 cagagccgat acgtagtgag ccatcttccg ccagcgtaat tccccgcagc tccgccagat
    21301 tatgaatatc aacaatatgg cgataacggt cattgtgatg gtggagctga atcagtacgt
    21361 cagtgccacc ggcgagcagt ttggcctgcg ggttgtcagc cagcaggttg atggcatcgg
    21421 caagggttgc tgcgcgatgg taagaagcaa aatcaaacat gatgttatcc tcaaatcaat
    21481 cctgccagat ggaactcttc atataaccgt tttggcgtca gcggcagtgt attgattgca
    21541 acaccggtag ccatcttcac cgcgttacga atagcagcgg caacaggaat tattggtggc
    21601 tcacccagtg acttatgtcc gtatgcggat tgcggctcat tgatttcgac gaacgcgctt
    21661 tccagttgtg gcagatccgg catggtcggc attttgtaat ccagcagatt ggggttacgg
    21721 accacgccgc ttttcgcatc aatgatcatc tcttcaaata gcgcccagcc aatgcccatt
    21781 cccattccgc cgtgtacctg accttctgcc agtagcggat taagaatatg gcccgaatca
    21841 tgaacgttga ggatgcggtt gatggtgact ttacacagcg caatatcgac cgtcagatca
    21901 acaaaggtac agccaaacgc tggtgggtta gtggtggttt tgatggagct tttggcagag
    21961 agctgcccgc cgcgttcagg gtggtagaag gcgtccatcg ccaaatcttt taacgacatt
    22021 aatggctctt ccggtcgttc aaccagcacg atatggcctt ttatcagggt aagattcatc
    22081 gctgactgat gtagcatgac agcggcatga gcgatgattt tctcctttaa taacagtgcc
    22141 gcactgcgca gcgcaggcgc ggcaacatag ctctggcgtg aggcaaatgc accgggatcg
    22201 aacggcgtaa cgtcggtatc ttgggttgaa ataacgcgaa cgtcgctgac cggaaccccc
    22261 acggtttctg ctaccatttg cgagaagacg gtgtcggccc cctgaccgat ttctgtcgcg
    22321 ccgctttgca cgttgatggt tccatcctga ttcatcaaca ggcgcgcgcc tgctatttct
    22381 acgccgacag gccaggtgtt agaggtgtag ctaaaacagg cgacgccaac gccgcggcgt
    22441 aaattgcctt gctggttctg gcactctgca cggcgttttt cccattcaaa gattttccgg
    22501 cctttttcaa gacactccgg caaccctgcg ctgtaaatac gtttgcccgt gagcggatta
    22561 gcatctcctt cgcgggcggc gttgcgtaaa cgaatttcaa caggatcaat acctaacgct
    22621 gtcgcggcgt catcaagcat agactcaacg gcaaatacga cttgtggcgc gccataacca
    22681 cgcatcgcac cagccgaggg gaggttggta tagcaggtct ttgaactgta agcgtaggca
    22741 caacgaggat aaaggtaagc gactttattt ccgcccgcag aagcgatgga gtgcccgtga
    22801 gatgcataag cgccggtgtt agacagaaca tccagactat aacctttcaa tgttccgtcg
    22861 cggttcacgc ccatttgccc gtcaatagta aaagcgtggc gggtacgggt tgcgaggaaa
    22921 cactcttcac ggctaaggga aactttcacc ggaatgccgc caagcttgct ggtcaggaat
    22981 gccgccattg gctcttccag tacatcctgt ttattaccaa aaccgccacc gacaaacggt
    23041 ttgatgactc gtacgcatga ccagggaata ttcagcgcct gaccaaccac acggcgaaca
    23101 atgtgcggga tctgggtgct ggaaacgatg gtaattcgcg agtcatcctc catccatgcc
    23161 agcgatgtca cgctttccat atgacaatgt tgaataacgg gagtctgata gtgcccctgc
    23221 acctggtaat cggcggcatc gattgtttgt tggacattac ccgtcgacat cgagctttgt
    23281 tttagtaaat tgccgccgtt atggattggt gcagcgtcat ctgccagcgc cgcttctggc
    23341 gtggtgataa cgggtaattc ttgccactca atgctgacca attgcgccgc tttttctgcc
    23401 gtgagttcat cacgggccac gacgatggca acggcgtcac catgatgacg aacatggcga
    23461 gttagcagtg cgcgatcggc ggtatcgcgc ttgttttcgt caagtgtcca ggcatgccca
    23521 gctgtagcga atgggatatc aggcacatct tcccaggtaa aaatcgccag tacgcccggt
    23581 aaacttctgg cttgttcatc attaatactt atggcataac catgtgcgat agggctacgt
    23641 acatatttcg cgtaacacat gcccgccata acataatcgt cagtatatcg tgcccgcccg
    23701 gtgaccttag caatggcatc tacgcgcatg catgattcac ctgtagcggt tgcttcccgc
    23761 gcttccatag aaatcccctc gatttgaaca tttggttctt atggcaaaga tttagtctaa
    23821 gcaggcaaga tttacgccag aaacgacgat aagcgttaag ggaggaagga agtgtgatga
    23881 tgttaaaaaa acacagatat gatacgaaac aggaccctga ataggggcag tgatacgtat
    23941 caatatgagt ggcgataaaa tcaatgtgat aaattgggat tattacgcat caggatggga
    24001 aatagggtaa atgagtcaga aaattcgata catctacagt gaatattttg ctgatgatac
    24061 ttccctttgc caaatgaaat taacctcttt ctctattacc gcgtttttcc agaagcaaga
    24121 gattgggtgt caagaggctg gctgctatga tatggcgtct tgttttttaa gcgaggaaag
    24181 attttgagtg cgggacgcct gaataaaaaa tctctgggta tcgtgatgtt gttatcgatt
    24241 ggactgcttt tggcgggctg ctcgggtagc aaatcatccg atacaggaac gtattccggc
    24301 tccgtttaca ccgtgaaacg gggggatacg ctatatcgta tttcgcgcac cacgggaact
    24361 agcgtaaaag agctggcgcg actgaacggc atttcccccc cttacaccat tgaagttggt
    24421 cagaaactaa aactgggtgg ggcgaaaaat agtagtacac gtaaatcaac cgccaaatca
    24481 acgaccaaaa ccgcatcggt tacaccgtca tcagcagtac cgaaatcttc ctggccgcca
    24541 gtagggcaac gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca
    24601 gctgatggcg gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc
    24661 gcgggtgcag gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc
    24721 atgattaaac acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta
    24781 aataatgggc aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cactgatgcg
    24841 gcatcggttc gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt
    24901 tacttgccgc ctcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca
    24961 gcgcggcact tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc
    25021 gtagttcaat ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc
    25081 ccgctccaaa caaacatcta ccaacgtcta ctaaagtctg ccaatccccc gaatatcaag
    25141 gcctccagcg attttcctcg tctacaagcg tctacagtca tctacctaaa tctacacaca
    25201 gaagtgtata gtaatgcgta taggacaaaa tcgttttttg ggggctatac acatgttgct
    25261 ttcagacctt gctatccgcc gcgcgaagcc aaaagagaaa gcctatacac tcaatgacgg
    25321 caacgggctt tcgttactca ttgagcctaa cggttcgaag ggctggcgat tgcgatacag
    25381 gtttgcaggt aagcccaaaa tgttgtcgct gggtgtttat cctgaggtgt ccctttctga
    25441 tgccagggcg gcaagggatg aagccaaaag gcttatcgct ggtggagtta atccaagtga
    25501 tgtgagaaaa gcccataaga gagaacaggc gagcaaattc ggtaacacct ttgaggcgat
    25561 cgcccgtgag tggtatgaaa aacgcctgga cagatggtca caatcatatg ccgcagagat
    25621 gattgatacg tttgagaagg acgtattccc gtatatcggc agcaaaccga tagcagaaat
    25681 aaaaccgatg gaattgctcg cggttctctc ccgtcttaat gaccgtggcg caactgaaaa
    25741 attgcgtaag gttcgccagc gatgtggcga agtgttccgg tacgcaataa ttaccggacg
    25801 cgctgaatat aaccccgctc ctgatctggc aagcgcattt gcaccacaca aaaaagagca
    25861 ttacgcgtat ctgaaagttg atgagctacc ggaatttttc cgcgttttta atacgtacac
    25921 cggaagccag atcgtcaagc tgggtatgcg attattgatt cttacaggac tccgcccagg
    25981 tgaattgcgg caggcagagt ggcaggagat tgattttgat aatcgtctgt gggaagtgcc
    26041 gaaagaacga atgaaaatgc gccgtcctca ttgcgtgccg ctttcagaac aggcgatagc
    26101 tattctggaa caactgaaac acatgacggg gcagtatccg tttattttcc ctggtcgaat
    26161 acagcacagt aaaccgatga gtgaaatggc gatgaacgtg cttattcgtc gtattggtta
    26221 tgcagggcgt gttactggtc atgggttccg gcacaccatg agtacgattt tgcacgagca
    26281 ggggtataac tccgcatgga tagaaacgca gctggcgcac gtcgataaaa actcgatccg
    26341 tggcacatac aatcacgcgc aatatctgga cggacggcgg gagatgctcc agtggtacgc
    26401 cgactatatg gactcactgg aacagggcgg aaacgtggta catggcaagt ttggaaaatg
    26461 tggatgactg gttgagtata cagtagtaga ctttgagcga ctaaataaca ggctgtgtct
    26521 agggtcgctc ccgaaacccc gtacacctct gcggactggc acagccacca ctacagagag
    26581 tgcgaggtgt gcacatgccg gttgtgaatg atttccctga cttaagtcat tggtttacag
    26641 tgcaggagtt tactattact caagctgcgt tattgctgtc aggtattgat cctctcgact
    26701 acccaactct ggagatggtc aaagaggaaa aacacgaacg atggagaatg gcatatgggt
    26761 ttgcttatgg aatggttact gcaattcgac gcggtgtctt aactcccgtt ctgtgcgttg
    26821 cgtatgattg gggggatatg cgtcgtttaa ccccagcaga gatgatggat cgttcaaata
    26881 atatatccat ggacggaaca attattaccc gttattcatt acaggaatgg gtaaaaaatg
    26941 agaaagttga ttatgtaaga ccctctcgct ctatatcgga taaggagatt aagccatcta
    27001 accaatcagt aataattgat gccgaatcta gcgtgattaa ttctgaagaa acagtattgc
    27061 tgacttatgg tcataagagc gaaggtcttg aatatgtaga agacgcaata agagaacttt
    27121 gggcaacata tgatccagat aatcaacaaa cagcccctac aagaaatgat gttgttgaat
    27181 acctgaaagg caggggagcg gggcagaaca tggccgaggc tgtaaacctg atactacgcc
    27241 ccactggctt gcagcaagtt ggaagaagga aaagaaaaac aaatagttaa atagtgccca
    27301 cccttcgtaa aaaaggtggc actatttgct caaaactctt ttgtttatgg cgagaaatca
    27361 tagttaagcg tgcgttttct ttgcttattg atactggaat ggtgg
//
LOCUS       DAZUCC010000046        27286 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZUCC010000046 DAZUCC010000000
VERSION     DAZUCC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.046"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHY09_001717"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHY09_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS3884700.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHY09_001718"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHY09_001718"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFS3884701.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHY09_001719"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHY09_001719"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFS3884702.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFNYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHNAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHY09_001720"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHY09_001720"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFS3884703.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHY09_001721"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHY09_001721"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS3884704.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6615..7109
                     /gene="lrp"
                     /locus_tag="ACHY09_001722"
     CDS             6615..7109
                     /gene="lrp"
                     /locus_tag="ACHY09_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFS3884705.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7244..11311
                     /gene="ftsK"
                     /locus_tag="ACHY09_001723"
     CDS             7244..11311
                     /gene="ftsK"
                     /locus_tag="ACHY09_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS3884706.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     ASVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAAEQSAQQPYYAPA
                     PEQTAAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQHPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAHHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11470..12081
                     /gene="lolA"
                     /locus_tag="ACHY09_001724"
     CDS             11470..12081
                     /gene="lolA"
                     /locus_tag="ACHY09_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFS3884707.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSNFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12092..13435
                     /gene="rarA"
                     /locus_tag="ACHY09_001725"
                     /gene_synonym="mgsA"
     CDS             12092..13435
                     /gene="rarA"
                     /locus_tag="ACHY09_001725"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFS3884708.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13526..14818
                     /gene="serS"
                     /locus_tag="ACHY09_001726"
     CDS             13526..14818
                     /gene="serS"
                     /locus_tag="ACHY09_001726"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS3884709.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15058..17502
                     /gene="dmsA"
                     /locus_tag="ACHY09_001727"
     CDS             15058..17502
                     /gene="dmsA"
                     /locus_tag="ACHY09_001727"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFS3884710.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17513..18130
                     /gene="dmsB"
                     /locus_tag="ACHY09_001728"
     CDS             17513..18130
                     /gene="dmsB"
                     /locus_tag="ACHY09_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFS3884711.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18132..18995
                     /gene="dmsC"
                     /locus_tag="ACHY09_001729"
     CDS             18132..18995
                     /gene="dmsC"
                     /locus_tag="ACHY09_001729"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFS3884712.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19031..19657)
                     /gene="ycaC"
                     /locus_tag="ACHY09_001730"
     CDS             complement(19031..19657)
                     /gene="ycaC"
                     /locus_tag="ACHY09_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFS3884713.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19972..21120
                     /gene="ycaD"
                     /locus_tag="ACHY09_001731"
     CDS             19972..21120
                     /gene="ycaD"
                     /locus_tag="ACHY09_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3884714.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21330..22760
                     /gene="ycaM"
                     /locus_tag="ACHY09_001732"
     CDS             21330..22760
                     /gene="ycaM"
                     /locus_tag="ACHY09_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS3884715.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(22761..22871)
                     /locus_tag="ACHY09_001733"
                     /pseudo
     CDS             complement(22761..22871)
                     /locus_tag="ACHY09_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(22970..23710)
                     /gene="pflA"
                     /locus_tag="ACHY09_001734"
     CDS             complement(22970..23710)
                     /gene="pflA"
                     /locus_tag="ACHY09_001734"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFS3884716.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMREVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(23902..26184)
                     /gene="pflB"
                     /locus_tag="ACHY09_001735"
     CDS             complement(23902..26184)
                     /gene="pflB"
                     /locus_tag="ACHY09_001735"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS3884717.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(26239..27096)
                     /gene="focA"
                     /locus_tag="ACHY09_001736"
     CDS             complement(26239..27096)
                     /gene="focA"
                     /locus_tag="ACHY09_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFS3884718.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         6714 a         6726 c         7169 g         6677 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta tggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggctaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag caccgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataattg aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgacg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagtttacc gccatgacca ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat agagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgact cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattcaggc caaagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcacctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcctcccagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgctta aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc atgcatcatt tctgcaagca attcaaggat
     1681 ctgactttcg gttgtggcat ctaagccttc tgtaggttca tccagcaata ccagcggcgc
     1741 attatgtaac agcgcacggg cgatagccag acggcgtagt tcaccaccgg agagctggcg
     1801 tccgccttca cccaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgtaag atctccgcca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagtcgct tcattcaggc tggctatggg gctaccgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataattga attgaacatc ccgtaacgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaagt cagtgatccg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtgag ttcagattga cgacgttgcg cttccagcca
     2521 ctgaatttct gtattctcta attgcgtgcg ataacgatcg ctggcaccaa agatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgcccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagt ggtggcataa ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggata ggggcagcaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaatagcgcc cggcagtacg
     3001 ggtgattgct gcgccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gctaccgctg aggcggagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtgacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagcaatgtg gcgaaaggac caccagccac gcttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcatta agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaatgct cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgtttgta actcgtgttc gctggcatca ggtcgtgcca gtagtacgtt
     3661 atcccgtagt gttgctgccg gtaattgtgg gttttgccca acccaggaga gatgcttacg
     3721 ccatgattcc ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcgaccaac
     3841 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccagcca gcgttttacc
     3901 ttctggtgat gtaataaaca gatcctcggc ttcaatggtc accggatcgg ttgatgctaa
     3961 ttccgcctca ccacgttgcg gatgggctag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcgcca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactcc ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 atagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga ataagcgccg cagcccagtt
     4501 agacgggaag atcgccacca caatcagcaa aggcacggac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgcc ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgctcacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gggatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgagga
     5041 aaaaataaag gcgacccata gtcgcatggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagcctgtac cagcagatgt aatagcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc cagggatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatag ccgtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac gcccatctga tcgccggtca cctcttccag cgtacggttg
     5461 gtgtgtagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt agcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gatggcaggc ctagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgc tatcgccagt cagacggaac ggacgattct gcaaatcaac tttgttgatg
     5821 tgatcaaaga tgatttcagt ttcaaatttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtatatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 cagtattctt atatgcgcat aaccatgcat gtaaatacca tgtttaccgt gctagtgaaa
     6421 tctacgtatg gcgtggacag acgccattcg tgatgtcgat agctgccaca aggcaacggt
     6481 cttctcaccg tagacccagg cattgcgcgc cgtgaatctt catgatttcg gtctatcgtg
     6541 acgggtagcg actctgaaca gtgatgtttc agggtcagac aggagtaggg aaggaataca
     6601 gagagacaat aataatggta gatagcaaga agcgccctgg caaagatctc gaccgtatcg
     6661 atcgtaacat tcttaatgag ttgcaaaagg atgggcgtat ttctaacgtc gagctttcta
     6721 aacgtgtggg actttcccca acgccgtgcc ttgagcgtgt gcgtcggctg gaaagacaag
     6781 ggtttattca gggctatacg gcgctgctga acccccatta tctggatgca tcacttctgg
     6841 tattcgttga gattactctg aatcgtggcg caccggatgt gtttgaacaa ttcaataccg
     6901 ctgtacaaaa acttgaagaa attcaggagt gtcatttagt atccggtgat ttcgactacc
     6961 tgttgaaaac acgcgtgccg gatatgtcag cttaccgtaa gttactgggg gaaaccctgc
     7021 tgcgtctgcc tggcgtcaat gacacacgga catacgttgt tatggaagaa gtcaagcaga
     7081 gtaatcgtct ggttattaag acgcgctaac acggaacagg tgcaaaatcg gcgtattttg
     7141 attacactcc tgttaatcca tacagcaaca gtactggggt aacctggtac tgttgtccgt
     7201 tttagcatcg ggcaggaaaa gcctgtaacc tggagagcct ttcttgagcc aggaatacac
     7261 tgaagacaaa gaagtcacat tgacaaagtt aagtagcggc cgccgccttc tggaggcgtt
     7321 gctgatcctt attgtcctgt ttgccgtctg gttgatggct gccttactaa gctttaaccc
     7381 ttcggacccc agctggtcgc aaacggcctg gcatgaacct atccataatt taggtgggat
     7441 gcccggtgcg tggctggcag atacgctgtt ctttattttt ggcgtgatgg cttacaccat
     7501 tcccgtcatt attgtcggcg gttgttggtt tgcctggcgt catcagtcca gcgacgaata
     7561 cattgattat tttgccgttt cgctacgcat cattggcgtt ttggcgctca tccttacctc
     7621 ctgtggtctg gcggcaatca acgctgacga tatctggtat tttgcctccg gtggcgtcat
     7681 tggcagctta ctaagcacta cgctacaacc actgctacac agtagcgggg gaactattgc
     7741 gctgctctgc gtttgggctg cgggtctgac gctgttcacc ggttggtcat gggtgaccat
     7801 tgctgaaaaa ctcggcggct ggattttaaa cattctcacc ttcgccagta atcgtacccg
     7861 tcgcgatgat acctgggtcg atgaagatga gtatgaagac gacgaagaat atgaagatga
     7921 aaatcacggt aaacagcatg aatcacgccg tgcccgtatt cttcgcggcg cgttagcgcg
     7981 tcgtaaacgg ttggcggaaa aattcattaa tccgatgggg cggcaaacag atgctgcgtt
     8041 gttctccggt aagcggatgg atgatgaaga agagattacc tacactgcac gcggtgtggc
     8101 tgccgaccct gacgacgtcc tcttttcggg caatcgtgca acgcagccag aatatgacga
     8161 atacgatcca ttattaaacg gtgcgccaat taccgaacct gttgctgtag cagctgctgc
     8221 taccacggcg acacaaagct gggctgcgtc ggttgaacct gtgactcaga cgccgcctgt
     8281 tgcctctgtt gatgttccac cttcgcaacc tacagtagcc tggcagcctg taccgggtcc
     8341 acaaacggga gagccggtga ttgctccggc accggaaggt tacccacagc agccacaata
     8401 tgcgcagcct gcagtgcaat ataatgagcc gctgcaacaa cctgtacagc cacagcagcc
     8461 gtattatgcg ccagcagcgg aacaacctgt gcaacaacct tactacgctc ctgcggcgga
     8521 acaacctgtt cagcagccgt attatgcaac tgcggcggaa cagtctgcgc aacagccgta
     8581 ttacgcccct gcgccagaac aaacggcggc aggtaacgcc tggcaagccg aagagcagca
     8641 atccactttt gctccacagt ctacatacca gactgagcaa acttatcagc agccagccgc
     8701 tcaggagccg ttgtaccaac agccgcaacc cgttgaacag caccctgttg tggagcctga
     8761 gcccgttgta gaagagacaa aacccgcgcg tccgccgctt tactattttg aagaagtgga
     8821 agagaagcgt gcccgtgaac gtgaacaact tgcggcctgg tatcaaccga ttcctgaacc
     8881 ggttaaagaa ccagaaccga tcaaatcttc gctgaaagca ccttctgttg cagcagtacc
     8941 tccagtggaa gccgctgccg ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact
     9001 ggcgacgggg gctgccgcaa ccgttgccgc gccagtcttc agtctggcaa atagcgctgg
     9061 accgcgtccg caggtcaaag aggggattgg cccgcagttg ccacgaccga aacgtatccg
     9121 cgtgccaact cgtcgtgaac tggcgtctta cggtattaag ctgccctcac agcgtgcggc
     9181 agaagaaaaa gcccgtgaag ctcagcgcaa tcagtacgat tccggcgatc agtataacga
     9241 tgatgaaatc gatgcgatgc agcaggatga actggcacgt cagttcgccc agacacagca
     9301 gcaacgctat ggcgaacagt atcaacatga tgtgcccgtg aacgcagaag atgcagatgc
     9361 tgcggcagag gctgaactgg ctcgtcagtt tgctcaaact caacaacaac gttattccgg
     9421 cgaacaacct gctggggcga atccgttcac tctggatgat tttgaatttt cgccaatgaa
     9481 agcgttgctg gatgatggtc cacacgaacc gctgtttacg ccaattgttg aacctgtaca
     9541 gcagccgcaa caaccgattg caccgcagca acagtatcag cagccgcagc aaccagttgc
     9601 gccgcagccg cagtatcagc agccgcaaca gccggttgca ccgcagcagc aatatcagca
     9661 gccacaacaa ccggttgcac cgcagccgca atatcagcag ccgcaacagc cggttgcacc
     9721 acagccgcaa tatcagcagc cgcaacagcc agttgcgccg cagccgcatg ataccctgct
     9781 tcatccgctg ttgatgcgca atggtgacag ccgtccgttg cataaaccga cgacgccgct
     9841 gccttctctg gatttgctga cgccgccgcc gagcgaagtg gagccggtag atacctttgc
     9901 gcttgaacaa atggcgcgcc tggtggaagc gcgtctggct gatttccgta ttaaagccga
     9961 tgtcgtcaat tattctccgg ggccggttat cactcgcttt gaattgaacc tggcaccggg
    10021 agtaaaagcg gcgcgtattt ctaacttgtc acgtgacctt gcccgttcac tttcgacggt
    10081 ggcggtgcgt gtcgttgaag ttattcctgg caaaccctat gtagggctgg agttaccgaa
    10141 taaaaaacga caaaccgttt atctgcgcga agttttggat aacgccaaat tccgcgataa
    10201 tccgtcgcca ttaaccgtgg tgctgggtaa agatatcgcc ggtgagccgg tggttgccga
    10261 tctggcgaaa atgccgcact tgttggttgc ggggactacc ggttccggta agtctgtcgg
    10321 tgtgaacgcg atgatcctga gcatgcttta taaagcacag ccagaagatg tgcgtttcat
    10381 catgatcgac ccgaaaatgc tggagctttc ggtttatgaa ggcatcccgc atctgttaac
    10441 ggaagtcgtt actgatatga aagatgccgc caacgcgctg cgctggtgtg ttaacgagat
    10501 ggagcgtcgt tataaactga tgtctgcgct gggtgtgcgt aatctggcgg gttataacga
    10561 aaaaattgct gaagccgatc gcatgatgcg tccgattcca gacccgtact ggaagccggg
    10621 tgatagtatg gatgcgcatc atccggtgct gaaaaaagaa ccgtacatag tggtgttggt
    10681 ggacgaattt gccgacctga tgatgacggt aggtaaaaaa gtggaagagc tgatagcacg
    10741 tctggcgcaa aaagcccgtg ccgcgggtat ccacctcgta ctggcaacgc agcgtccatc
    10801 ggttgatgtt attactggtc tgattaaagc gaatattccg acccgtatcg cctttaccgt
    10861 atccagtaag attgactcac gtaccattct tgatcaggct ggcgcggaat cactgctggg
    10921 tatgggggat atgctctact ctgggccgaa ctccacgttg ccggtacgtg tccatggtgc
    10981 ttttgtacgc gatcaggaag ttcatgccgt ggtgcaggac tggaaagcgc gtggtcgccc
    11041 acagtatgtt gacggcatca cctccgacag cgaaagcgaa ggtggtgcgg gtggtttcga
    11101 tggcgctgaa gaactggatc cgttgttcga tcaggcggtg cagtttgtca ctgaaaaacg
    11161 caaagcgtca atttcaggcg tacagcgtca gttccgcatt ggttataacc gtgcagcgcg
    11221 tattatcgaa cagatggaag cgcaagggat tgtcagcgaa caggggcaca acggtaatcg
    11281 tgaagtgctg gccccaccgc cgtttgacta attaatgcat cgtaggccgg ataaggcgcg
    11341 gtagcgtcgc atccggcact ctaacaactg aaaattcagt attttcttct ttcctcaagc
    11401 tgattattag cctggaatag agagtagagg gaactcccga tcgggagtga cgtaatttga
    11461 ggaataatga tgaaaaaaat tgccatcacc tgtgcattac tctcaagctt agtagcaagc
    11521 agcgtttggg ctgatgccgc aagcgatctt aaaagccgcc tggataaagt cagcaacttc
    11581 cacgccagct tcacacaaaa agtgactgac ggtagcggcg cggcggtgca ggaaggccag
    11641 ggcgatctgt gggtgaaacg tccaaattta ttcaactggc atatgactca acctgatgaa
    11701 agcattctgg tttctgacgg taaaacactg tggttctata acccgttcgt tgagcaagct
    11761 acggcaacct ggctgaaaga tgccaccggt aatacgccgt ttatgctgat tgcccgcaac
    11821 cagtccagcg actggcagca gtacaatatc aaacagaatg gcgatgactt tgtcctgacg
    11881 ccgaaagcca gcaatggcaa tctgaagcag ttcaccatta acgtgggacg tgatggcaca
    11941 atccatcagt ttagcgcggt ggagcaggac gatcagcgca gcagttatca gctgaaatcc
    12001 cagcaaaatg gggctgtgga tgcagcgaaa tttaccttca ccccgccgca aggcgtcacg
    12061 gtagatgatc aacgtaagta gaggcacctg agtgagcaat ctgtcgctcg atttttcgga
    12121 taatactttt caacctctgg ccgcgcgtat gcggccagaa aatttagcac agtatatcgg
    12181 ccagcaacat ttgctggctg cggggaagcc gttgccgcgc gctatcgaag ccgggcattt
    12241 gcattctatg atcctctggg ggccaccggg taccggcaaa acaaccctcg ctgaagtgat
    12301 tgcccgctat gcgaacgctg atgtggaacg tatttctgct gtcacctctg gcgtgaaaga
    12361 gattcgcgag gcgattgagc gcgctcggca aaaccgcaat gcaggtcgcc gcactattct
    12421 ttttgttgac gaagttcacc gtttcaacaa aagccagcag gatgcatttc tgccacatat
    12481 tgaagacggc accatcactt ttattggcgc aaccactgaa aacccgtcgt ttgaacttaa
    12541 ctcggcactg ctttcccgtg cccgtgtcta tctgctgaaa tccctgagta cagaggatat
    12601 tgagcaggtg cttactcagg cgatggaaga caaaacccgt ggctatggtg gtcaggatat
    12661 tgttctgcca gatgaaacac gacgtgccat tgctgaactg gtgaatggcg acgcgcgccg
    12721 ggcgttaaat acgctggaaa tgatggcgga tatggccgaa gtcgatgata gcggtaagcg
    12781 ggtcctgaag cctgaattac tgaccgaaat cgccggtgaa cgtagcgccc gctttgataa
    12841 caaaggagat cgcttttacg atctgatttc cgcactgcat aagtcggtac gtggtagcgc
    12901 acccgatgcg gcgctgtact ggtatgcgcg aattattacc gctggtggcg atccgttata
    12961 tgtcgcgcgt cgctgtctgg cgattgcgtc tgaagacgtc ggtaatgccg atccacgggc
    13021 gatgcaggtg gcaattgcgg cctgggattg ctttactcgc gttggcccgg cggaaggtga
    13081 acgcgccatt gctcaggcga ttgtttacct ggcttgcgcg ccaaaaagca acgctgtcta
    13141 cactgcgttt aaagccgcgc tggcggatgc acgcgaacgt ccggattatg acgtgccggt
    13201 tcatttgcgt aatgcgccga cgaaattaat gaaggaaatg ggctacgggc aggaatatcg
    13261 ttacgctcat gatgaagcaa acgcttatgc tgccggtgag gtttatttcc cgccggaaat
    13321 agcacaaaca cgctattatt tcccgacaaa caggggcctt gaaggcaaga ttggcgaaaa
    13381 gctcgcctgg ctggctgaac aggatcaaaa tagccccata aaacgctacc gttaatgtta
    13441 tcgttgcggt aatgttgtta ctgtatccct gtggtcgcag gctgtggcca catctcccat
    13501 ttaattcgat aagcacagga taagcatgct cgatcccaat ctgctgcgta atgagccaga
    13561 cgcagtcgct gaaaaactgg cacgccgggg ctttaagctg gatgtagata agctgggcgc
    13621 tcttgaagag cgtcgtaaag tattgcaggt caaaacggaa aacctgcaag cggagcgtaa
    13681 ctcccgatcg aaatccattg gccaggcgaa agcgcgcggg gaagatatcg agcctttacg
    13741 tctggaagtg aacaaactgg gcgaagagct ggatgcagca aaagccgagc tggatgcttt
    13801 acaggctgaa attcgcgata tcgcgctaac catccctaac ctgcctgcag atgaagtgcc
    13861 ggtaggtaaa gacgaaaatg acaacgttga agtcagccgc tggggcaccc cgcgtgagtt
    13921 tgactttgaa gttcgtgacc atgtgacgct gggtgaaatg cactctggcc tcgactttgc
    13981 agccgcagtt aagttgaccg gttcccgctt tgtggtaatg aaagggcaga ttgctcgcat
    14041 gcaccgcgca ctgtcgcagt ttatgctgga tctgcatacc gaacagcatg gctacagtga
    14101 gaactatgtt ccgtacctgg ttaaccagga cacgctgtac ggtacgggtc agctgccgaa
    14161 atttgctggc gatctgttcc atactcgtcc gctggaagaa gaagcagaca ccagtaacta
    14221 tgcgctgatc ccaacggcag aagttccgct gaccaacctg gtgcgcggtg aaatcatcga
    14281 tgaagatgat ctgccaatta agatgaccgc ccacacccca tgcttccgtt ctgaagccgg
    14341 ctcatatggt cgtgacaccc gtggtctgat ccgtatgcac cagttcgaca aagttgaaat
    14401 ggtgcagatc gtgcgcccgg aagactcaat ggcggcgctg gaagagatga ctggtcatgc
    14461 agaaaaagtc ctgcagttgc tgggcctgcc gtaccgtaaa atcatccttt gcactggcga
    14521 catgggcttt ggcgcttgca aaacttacga cctggaagta tggatcccgg cacagaacac
    14581 ctaccgtgag atctcttcct gctccaacgt ttgggatttc caggcacgtc gtatgcaggc
    14641 acgttgccgc agcaagtcgg acaagaaaac ccgtctggtt cataccctga acggttctgg
    14701 tctggctgtt ggtcgtacgc tggttgcagt aatggaaaac tatcagcagg ctgatggtcg
    14761 tattgaagta ccagaagttc tgcgtccgta tatgaacgga ctggaatata ttggctaata
    14821 cccaattttt ctgaatctaa aaagcgccct gcgggcgctt tttttgtctc cctttgatac
    14881 cgaacaataa ttactcctca cttacacgta atactacttt cgagtgaaaa tctacctatc
    14941 tctttgattt tcaaattatt cgatgtatac aagcctatat agcgaactgc tatagaaata
    15001 attacacaat acggtttgtt actggaatca atcgtgagca agcttgagtg agccattatg
    15061 aaaacgaaaa tccctgatgc ggtattggct gctgaggtga gtcgccgtgg tttggtaaaa
    15121 acgacagcga tcggcggcct ggcaatggca agcagcgcgt taacattacc ttttagtcgg
    15181 attgcgcacg ctgttgatag tgcccttccg gcaaaatcag acgaaaaggt tatctggagc
    15241 gcctgtacag ttaactgtgg tagtcgctgc ccgctacgta tgcacgttgt ggacggtgaa
    15301 attaaatatg ttgaaacgga taataccggc gatgacaact atgacggcct gcaccaggtg
    15361 cgcgcctgtc tgcgtggtcg ttccatgcgt cgccgtgtct acaatccgga ccgcctgaaa
    15421 tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa
    15481 gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta cggcaacgag
    15541 tctatctatc tgaactatgg taccggaacg ctgggcggca ccatgacccg ctcctggccg
    15601 ccgggaaata ccctggtcgc tcgcctgatg aactgctgcg gcggttatct gaaccattac
    15661 ggtgactact cttccgcgca aattgcggaa ggtttgaact atacctacgg cggctgggca
    15721 gatggtaaca gcccgtcgga tatcgaaaac agtaaactgg tagtgctgtt tggtaataac
    15781 cctggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca ggcgcgccag
    15841 aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg tgccgggcgc
    15901 gaagatgagt ggatccctat tcgtccggga acagatgccg cactggttaa cggtctggcg
    15961 tatgtcatga tcaccgaaaa cctggtggat caggcattcc tcgataaata ttgcgttggc
    16021 tacgacgaga aaacactgcc agccagtgcg ccgaaaaatg gtcactataa agcttatatt
    16081 ctgggtgaag ggccagatgg cgtggctaaa acgccggaat gggcctcgca aatcactggt
    16141 gttccggcag acaaaatcat caaactggct cgtgaaatcg gtagtaccaa accggcgttt
    16201 atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc
    16261 tcgatgctgg cgattctgac cggtaacgtt ggtattaacg gaggcaacag cggcgcgcgt
    16321 gaaggttcat acagcttacc gtttgtccgt atgccgaccc tcgaaaaccc gatccagacc
    16381 agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat gacagcgctg
    16441 cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat ctggaactat
    16501 gccggtaact gcctgattaa tcagcattct gaaatcaacc gtacccatga aatccttcag
    16561 gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc atcggcgaaa
    16621 tatgctgaca tcctgctgcc tgactgcacc gcttcagaac agatggactt tgcactggat
    16681 gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt
    16741 gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa
    16801 cagtttactg agggccgtac ccaggaagag tggatgcggc atctgtatgc ccagtcgcgg
    16861 gaagcgattc ctgaactgcc aacgtttgaa gagttccgta agcaggggat ctttaaaaag
    16921 cgcgacccac aagggcatca cgttgcttat aaagctttcc gtgaagatcc gcaggcaaat
    16981 ccactgacca cgccatcggg gaaaattgag atttattcgc aggcgctggc tgacattgcc
    17041 gctacctggg aattgccaga aggtgatgtg atcgatccac tgccgatcta cacgccgggc
    17101 tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac gggtttccac
    17161 tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc agcttgccgt
    17221 caggaaatgt ggatcaaccc gcttgatgcg caaaaacgcg gtatcaacaa cggcgataaa
    17281 gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac gccacgaatg
    17341 atgccgggtg tggtcgcact gggtgaaggt gcctggtatg acccggatac aaaacgtgtc
    17401 gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct cgctaagggg
    17461 aatccgtcac atacaaacct ggttcaggtt gaaaaggtgt aaggagtaac cgatgacaac
    17521 ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct gcgagctggc
    17581 ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt atgaatatgc
    17641 tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg cctactatct
    17701 gtcgatttca tgtaaccact gcgaagatcc ggcttgtact aaagtctgcc cgagcggtgc
    17761 gatgcataaa cgtgaagatg gttttgttgt ggtcgatgaa gatgtgtgca ttggctgccg
    17821 ttactgccat atggcttgcc cgtacggcgc accgcaatat aacgaaacga aaggccatat
    17881 gaccaaatgc gatggttgtt atgaccgtgt tgcggagggg aaaaagccga tctgtgttga
    17941 atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta aaaaacatgg
    18001 cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga atattgtgat
    18061 caaacccaat gccaacagcc gcccgaccgg ggataccact ggctatctgg caaacccgaa
    18121 ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc acggtcttcg
    18181 ggcaatgtgt agcgggtggt tttatcgttc tggctttggc gctgctcaaa ggcgacctgc
    18241 gagcagaagc ccagcagcgt gttattgcct gcatgtttgg tttatgggtg ctgatgggca
    18301 ttggctttat cgcctctatg cttcatcttg gttcaccgat gcgcgccttt aactcgctca
    18361 accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc ttttttgctg
    18421 ttggtggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca ttgcgtacgc
    18481 tgtggctgat agtgacgatg gttcttggcg tcatctttgt ctggatgatg gtgcgtgtgt
    18541 ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg ggcttcttcc
    18601 tgacgatgtt catgggcggc ccgttactgg gttacctgtt gctgagtctg gctggcgtcg
    18661 atggctgggc gatgcgtctg ctgccagcca tttctgtact ggcactggta gtgagtggcg
    18721 tgatgtcagt gatgcagggc gcagagctgg cgactattca tagctctgtg cagcaggccg
    18781 cagcgctggt gccggactat ggcgcgctga tgtcctggcg gatcgtgctt ttggctgttg
    18841 ccctgtgctt gtggattgca ccacagctaa aaggttatca gcctgcggtt ccgctacttt
    18901 ctgtatcatt cattctgctt ctggcagggg aattaatcgg tcgtggcgta ttctatggtt
    18961 tgcatatgac cgtggggatg gctgtcgcaa gttaatcagt aacaaccggg gtttcggccc
    19021 cggttcgctt ttatttctgc ttcgttaacg tgtcataact ggtcatcaag ttacgataat
    19081 caggaatgtg gttagagaac aacgtcgcca gtccttcaat atcattacgc cagtcgcgat
    19141 gcagctcgca tgccacgccg aaccatgtca tcaattgcgc accagcttgc gacatgcgat
    19201 cccatgccga atgccgggta atttcattaa aggtccctga cgcgtcagtc acgacaaaga
    19261 cgtcaaaccc ttcttcaatg gctgaaagcg ccgggaatgc cacgcaaact tcggttacca
    19321 caccggcaat aattaactgt tttttacctg tcgctttgac agcttttaca aaatcttcgt
    19381 tatcccaggc gttaatattt cccgggcgag caatgtaagg tgcatcgggg aattgtgctt
    19441 tcagttctgg aactaatggg ccgttaggac cggtttcaaa actggtggta agaatagttg
    19501 gtaagttgaa atacttagcc aggtcaccca gcgccagcac gttattttta aacttatcgg
    19561 gttcgatatc ccgtacaagg gaaagtaaac cagcctggtg atcaacaagc aaaacggcag
    19621 catcattttt atcaagacga acatacggtt tggtcatcct cttctccttt cgagagtcag
    19681 tggtacgtct gaaatcggtc agacgcgatg cattgctctg aaagcataga cgggaaatat
    19741 gagtttgctg tgaccatgaa atttttcgac tgaacgaagc gtatgaaatt tgtgttagat
    19801 caataaaaac aatcagatga gattaatcgt ctctccagca ttagcgttat gaatgagtaa
    19861 tataaatcga taatacctcc gctgcgatat gcgcgtattt tcacccattg acaatgtttt
    19921 tggcggtggc atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg
    19981 tatacccggc ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgatcgca
    20041 gtgttaaata cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta
    20101 ggcgttgtca gctcatccta ttttaccggc aacctggtcg gtacattgct gacagggtat
    20161 gtcattaagc gcattggctt taaccgcagc tattatctgg cctccttcat ttttgccgct
    20221 ggctgtgccg gccttggcct gatgattgga ttctggagct ggttggcttg gcgttttgtc
    20281 gccggtgtcg gttgtgccat gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg
    20341 acgtcacgta accgtgggcg tttgcttgct gcgtatatga tggtttatta cgtgggaacg
    20401 tttttaggcc agttactggt cagcaaagtt tcaaccgagc tgatgtccgt attgccgtgg
    20461 gttacaggtt tgacgttggc agggatctta ccgctgttgt ttacgcgtgt gctgaatcag
    20521 caggctgaaa accatgattc gacgtcaatt acgtcaatgc taaaactccg tcaggcgcgg
    20581 cttggcgtga atggctgcat tatctcagga atcgtgctgg gatctctata tggcctgatg
    20641 ccgctgtacc tcaatcacaa aggggtgagc aatgccagca ttggtttctg gatggcggta
    20701 ctggtcagtg cgggtatcct cggacaatgg ccgatcggac gtctggcgga taagtttggt
    20761 cgactgctgg tgttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat cgcgatgctt
    20821 agccaggcgg cgatggcccc agcgttattc atcctcggtg ccgctggctt tacgctatat
    20881 ccggtggcga tggcctgggc ttgtgagaaa gttgaacatc atcaactggt ggcgatgaac
    20941 caggccttac tgttgagcta taccgtggga agtctgcttg gcccgtcatt taccgctatg
    21001 ctaatgcaga atttctccga taatttattg tttatcatga tcgccagcgt atcgtttatc
    21061 tatttgctga tgctgcttcg caacgccggt catacgccga aacccgttgc tcacgtgtaa
    21121 atgaattcaa gcagagtgtg aacttactgt ttcacactct gcttttttgt ttcttctatc
    21181 tgacttgctt tattccaaat tttattcgtt taaaaataaa atgtgcagca ggttataatt
    21241 ttgcatttcg ctatttccgc acttcttatt tgccgcgcat aatccctcgt tttacagatg
    21301 cccctttaat tttggcgaag gatttgtcta tggctgggaa tgttcaggaa aaacagctgc
    21361 gatggtacaa cattgcgctg atgtctttta tcactgtctg gggttttggc aatgttgtta
    21421 ataactacgc caaccagggg ctggtggttg ttttttcatg ggtgtttatc tttgcgctct
    21481 atttcacacc ttatgcgcta attgttggtc agttaggctc gaccttcaaa gatgggaagg
    21541 gcggggtcag tacctggatt aaacacacga tgggacccgg actggcttac ctcgccgcgt
    21601 ggacctactg ggtagtgcat attccctatc tggcacaaaa accccaggca atactgattg
    21661 cgctcggttg ggcgatgaaa ggcgacggtt cgttaatcaa agaatattca gtcgtagcgt
    21721 tacaggggtt aacgctggtg ctgtttatct tctttatgtg ggttgcttca cgcggtatga
    21781 aatcgctgaa aatcgtcggt tctgtggcag ggattgcgat gtttgttatg tcgctcctgt
    21841 atgtggcgat ggcggtaacc gcgcctgcaa ttactgaagt gcatattgcg accacaaaca
    21901 ttacctggga aacgttcatt cctcatatcg actttaccta cattaccact atttcaatgc
    21961 tggttttcgc ggttggcgga gcagagaaga tttctcctta cgttaatcaa acgcgcaacc
    22021 caggaaaaga atttccaaaa gggatgttat gcctggcggt gatggttgcg gtttgtgcca
    22081 ttctgggttc actggcgatg gggatgatgt ttgactcgcg taatatcccg gatgacttaa
    22141 tgactaacgg tcagtattac gcctttcaga agctgggcga gtattacaac atgggtaata
    22201 ctttaatggt gatttacgcc attgcgaata ccctgggaca agtagctgcg ctggtattct
    22261 cgattgatgc cccgcttaaa gtgctattag gcgatgctga cagcaaatat attccagcca
    22321 gtttatgtcg taccaacgct tctggtacgc ccgttaatgg ctattttctg accctggtgc
    22381 tggtggcgat cctgataatg ctaccgactc tcggcattgg tgatatgaac aatctctata
    22441 aatggctgtt gaatcttaac tcggtagtga tgccgctgcg ttatttatgg gtatttgttg
    22501 catttattgc agttgttcgc ctggcgcaga aatataagcc ggaatatgtc tttattcgta
    22561 ataagccgct ggcaatgacc gtcggaattt ggtgttttgc ctttaccgct tttgcctgcc
    22621 tgactggtat tttcccgaaa atggaagcct tcaccgcaga gtggacattc cagttggcgc
    22681 tgaatgttgc aacgccgttt gtgctggtag gattagggct gatattcccg ctgctggcgc
    22741 gtaaagcgaa tagcaaataa ttattgtggt cggtgatgct gactctggca gatctgaccc
    22801 gtttttacat aatcgagaaa tgcgcgcaag ccgcaggaca tatactggcg gtttgggtaa
    22861 tacatttgga agctgtgctg taagggcgtt agaaatagag ctctgaatta tttatgcagc
    22921 gcggaataaa aaagcggcaa ctcaaaaaag ttgccgcttt acggggaaat tagaacatta
    22981 ccttatgacc gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg
    23041 gtttaacacc atcaagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt
    23101 cgtggtaggg gagaagctcg attttctcaa cgttgcccat atcacgggta aattcaccga
    23161 ggcgatgcgc tgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca
    23221 ccttcacatt tttgttcgcc agatatttgg cgaactccag cgtgcggtgg ttggaaactc
    23281 caaccagatt ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg
    23341 ttacttccag cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtgtcca
    23401 gacaggtatg aatgccttct tttttgcagg cgcggaacca gtcacgaaca aactcagctt
    23461 gcaggattgc ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc
    23521 gataggtcac cacctctctc attaaatctt caacggtaac ttctttaccg ccatgcgtgt
    23581 cccaggtgtc gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga
    23641 taaagcgaat acccggaccg tctacggttc cacaggattc aaaggagtga atgcgaccaa
    23701 taactgacat tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc
    23761 tcgaaggcta cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa
    23821 aggctctcgt gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg
    23881 tacgatttca gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg
    23941 ctgctgttct ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag
    24001 ctgcggatat ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt
    24061 aacgttcagg tgctgaccac cttcgatgga cgcttcgtgg tggaagtaac catccatcag
    24121 accagccagg ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga
    24181 gaaggtgtag gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag
    24241 agaggctact gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg
    24301 cgcgccagca cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga
    24361 agtgatggtc agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat
    24421 tttcttcatg aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcgttgtt
    24481 accaaactgc gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg
    24541 aatcggttta actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc
    24601 agcgatacca cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc
    24661 ttcgtagctg tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt
    24721 agccagccag tccatgaagt gatccatgcg ctccatcact tcatcgtagt tcaggagatc
    24781 gcctttgatc ggctcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc
    24841 gttgattgcg tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg
    24901 tttaccaacg atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc
    24961 cggacgcatc aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc
    25021 agcgaatttc ttgaagttca gcggcagttt ttcggaccac agaatggtca tgttcggttc
    25081 cggagacgga cccatggtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag
    25141 ggtacgaccg tcgaggccca taccaccgat agattcggtt gcccagatcg ggtcgccaga
    25201 gaacagttca tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag
    25261 gtggtcaacc atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc
    25321 gatgtacaca tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga
    25381 cttaacagca gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc
    25441 cggaccagag atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg
    25501 gtgctgttca gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc
    25561 cagatcagcc tgcagagagg tgaactgagc gtatttgtct ttcatcagat agtcgatacc
    25621 gtacagcgca acgcgacggt agtcaccgat gatacgacca cggccataag catctggcag
    25681 accggtcaga acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac
    25741 gccctggttg tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc
    25801 gcggttgtac gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc
    25861 acgtttcagc ggagcttcag tctgcagacc aacaactttt tccaacgctt tgttgatgta
    25921 gccagcgtcg tgagaggtga tggtggaagc aacagcggtg tcaaagtcaa ctggcgcgtg
    25981 agtgcggttt tccagtttaa cgccttccat tactttgtcc cacagggtgg tggtcgcttc
    26041 agtagcgcca gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa
    26101 gtcacggacg tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc
    26161 taacttttca ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac
    26221 tgcgtacttc gacaaccatt aatggtgatc gttttcacgc agataaatga cccagtatgt
    26281 caacccaacc aacaaaccac cgccgataat attaccgatc gtaaccggaa tcaggttatc
    26341 agtgatgaag ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt
    26401 ccagaattcc ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt
    26461 tgcgatactg tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa
    26521 cgctttatcc atcaggctgc ggccagaata actcatccat actgccagac ataccatcag
    26581 gtttgccagg ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc
    26641 ggtttgtagg acgtttagtc cccattgacc attcgcggtc atatactcgc cggaaagcca
    26701 cattaaaagt acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt
    26761 tttcgctaac tgaccccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt
    26821 ggaagtaaag agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat
    26881 gccgccaacc agttttgcca tgccgaaggg cattgttcct gtgccagtgg ttgctgtgat
    26941 atagaagacg aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt
    27001 aagcggatgt ttcgttgctt tatagacacc tgcctcttcg gccactttgg ccattgctgc
    27061 aggaagtaaa agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg
    27121 gcgacgagat actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg
    27181 gcttataggc ccgtaactcg catggttttt atgcaaatac ggagtaaata tttgattatc
    27241 caaataaaaa taaattttaa aaattaacaa atgagttgaa tttttt
//
LOCUS       DAZUCC010000047        27194 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZUCC010000047 DAZUCC010000000
VERSION     DAZUCC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.047"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..662
                     /gene="rcsB"
                     /locus_tag="ACHY09_001737"
     CDS             12..662
                     /gene="rcsB"
                     /locus_tag="ACHY09_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFS3884719.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(748..3597)
                     /gene="rcsC"
                     /locus_tag="ACHY09_001738"
     CDS             complement(748..3597)
                     /gene="rcsC"
                     /locus_tag="ACHY09_001738"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFS3884720.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            3764..5590
                     /gene="atoS"
                     /locus_tag="ACHY09_001739"
     CDS             3764..5590
                     /gene="atoS"
                     /locus_tag="ACHY09_001739"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFS3884721.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            5587..6972
                     /gene="atoC"
                     /locus_tag="ACHY09_001740"
     CDS             5587..6972
                     /gene="atoC"
                     /locus_tag="ACHY09_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFS3884722.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSNETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            7168..7830
                     /gene="atoD"
                     /locus_tag="ACHY09_001741"
     CDS             7168..7830
                     /gene="atoD"
                     /locus_tag="ACHY09_001741"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFS3884723.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            7830..8480
                     /gene="atoA"
                     /locus_tag="ACHY09_001742"
     CDS             7830..8480
                     /gene="atoA"
                     /locus_tag="ACHY09_001742"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFS3884724.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            8477..9799
                     /gene="atoE"
                     /locus_tag="ACHY09_001743"
     CDS             8477..9799
                     /gene="atoE"
                     /locus_tag="ACHY09_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFS3884725.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPIMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            9830..11014
                     /gene="atoB"
                     /locus_tag="ACHY09_001744"
     CDS             9830..11014
                     /gene="atoB"
                     /locus_tag="ACHY09_001744"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFS3884726.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATKKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(11088..11864)
                     /gene="yfaP"
                     /locus_tag="ACHY09_001745"
     CDS             complement(11088..11864)
                     /gene="yfaP"
                     /locus_tag="ACHY09_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFS3884727.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(11869..13518)
                     /gene="yfaQ"
                     /locus_tag="ACHY09_001746"
     CDS             complement(11869..13518)
                     /gene="yfaQ"
                     /locus_tag="ACHY09_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFS3884728.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVNSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVLVELFARYPLKKITAEKSS
                     TAVKPGVLNGRYRVTFANGNHIMFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTFQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(13519..18033)
                     /locus_tag="ACHY09_001747"
     CDS             complement(13519..18033)
                     /locus_tag="ACHY09_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFS3884729.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDEDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARIPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTEKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIQLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPDYWQHMLDSYAEQADNMPILHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGKLAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(18057..18680)
                     /gene="yfaT"
                     /locus_tag="ACHY09_001748"
     CDS             complement(18057..18680)
                     /gene="yfaT"
                     /locus_tag="ACHY09_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFS3884730.1"
                     /translation="MRHGLLALICWLCCVVTHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(18677..20365)
                     /gene="yfaA"
                     /locus_tag="ACHY09_001749"
     CDS             complement(18677..20365)
                     /gene="yfaA"
                     /locus_tag="ACHY09_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFS3884731.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKAQAVQPDQLMSDYFFRVSLA
                     MQNKTMLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(20514..23141)
                     /gene="gyrA"
                     /locus_tag="ACHY09_001750"
     CDS             complement(20514..23141)
                     /gene="gyrA"
                     /locus_tag="ACHY09_001750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFS3884732.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLIIQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            23288..24010
                     /gene="ubiG"
                     /locus_tag="ACHY09_001751"
     CDS             23288..24010
                     /gene="ubiG"
                     /locus_tag="ACHY09_001751"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFS3884733.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVEYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(24150..>27194)
                     /gene="yfaL"
                     /locus_tag="ACHY09_001752"
     CDS             complement(24150..>27194)
                     /gene="yfaL"
                     /locus_tag="ACHY09_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFS3884734.1"
                     /translation="YGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSASGYDHS
                     ATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAGELVLNA
                     DNNAFTGEISIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEYQAELNV
                     GNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSYLLTGAQ
                     SMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDGDSSYND
                     GLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLANNNGYL
                     GNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTGQGRNIE
                     MRADGEIHVEDGVDTQWGGLMADSTGQQLESVSTLTKSGGGTLELTASGTATSAVRVE
                     DGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTVLRLTQQ
                     DTNQVLDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNTSGAI
                     SLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTAVVINPITG
                     IGEPTSTGIKVVDFAADPTQFQNNAQFRLAGSGYVNMGAYDYTLVEDNNDWYLRSQEV
                     TPTPPPDPDPTPDPDPTPDPDPTPDPGPTPAYQPVLNAKVGGYFNNLRAANQAFVMER
                     RDQAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGHWGDDGEWMLGI
                     VGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGVWLDSWLQYAWFNN
                     DVSEQDDGEDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQDDFTAANHAR
                     VSQSQGDDIQTRLGLHSEWSTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDGVKQRG
                     EIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         6404 a         7441 c         6732 g         6617 t
ORIGIN      
        1 gatgcaagta catgaacaat atgaacgtaa ttattgccga tgaccatccg atagtcttgt
       61 tcggtattcg caaatcactt gagcaaattg agtgggtgaa tgttgtcggc gaatttgaag
      121 actctacagc actgatcaac aacctgccga aactggatgc gcatgtgttg attaccgatc
      181 tctccatgcc tggcgataag tacggcgatg gcattacctt aatcaagtac atcaagcgcc
      241 atttcccaag cctgtcgatc attgttctga ctatgaacaa caacccggcg attcttagtg
      301 cggtattgga tctggatatc gaagggatcg tgctgaaaca aggtgcaccg accgatctgc
      361 cgaaagctct cgccgcgctg cagaaaggga agaaatttac cccggaaagc gtttctcgcc
      421 tgttggaaaa aatcagtgct ggtggttacg gtgacaagcg tctctcgcca aaagagagtg
      481 aagttctacg cctgtttgcg gaaggtttcc tggtgaccga gatcgctaaa aagctgaacc
      541 gcagtatcaa aaccatcagt agccagaaga aatctgcgat gatgaagctg ggtgtcgaga
      601 acgatattgc tctgctgaat tatctctctt ctgtgacgtt aagtccggca gataaagact
      661 aatcacctgt aggccagata agacgcgtca gtgtcttatc tggcatttgc acggattgcc
      721 ggatgcggcg taaacgcctt atccgtccta cgattcccgc gatttcctga ccctctcggc
      781 atataccgtc agcgtctgtt ttatcacatc cagcgttacc ggcttcgaca ggcagctgtc
      841 cataccggac tccagacacc gctgcttctc ttcagccaac gcattagcag ttactccgat
      901 taccggcaac gtcagtccca actgacgaat gcgttgcgtc aagcggtaac catccatatt
      961 tggcatgttg acgtcgctaa gcacgatatc aatatgattc ttgctaagta cattaagcgc
     1021 atcgacgcca tcattcgcgg ttttacattg atagcccaac gatcccaact gatctgccag
     1081 cagacgccgg ttaatcggat gatcatccac gaccagaatc atcatatcgt cattatcgct
     1141 gaccgctttg tccgttaacg gcagagcgtt agcaggatcg tcgctctcca tctcgatcaa
     1201 ataaatacgc gccaacaatg ccggtagctc atgcggggca gccacactgt gtacccactc
     1261 tcctggcgct ttctccagcg gaataccaat atggcgacga cagaaggtca caaccgctct
     1321 accctgccat tttttgctca ctacctcgtc agtgattaac acatcttcgg gagtcggttc
     1381 ctgcccttcg tatgttgtaa cgacgatgcc gctgcgctgc aaactggttt ccaggaactg
     1441 acagagcgac gcattgcgga ccgccagcca gcaacgttta ccactcaacc cttccacgcc
     1501 ttttttctgc gggtactgag cgccgtacaa cggaatacgc acggtaaact ggctgcccat
     1561 tcccggttct gaatctaccg agatgtcgcc gtccatcatg ctgatcagtt tttcacaaat
     1621 cgccagaccc agaccggtcc cctggaagtt acgctgtacg cccgttccga cctggaagaa
     1681 gggatcaaac aagcgcacca cttctttcgc cggaatccct acgccggtat cgcgaacacg
     1741 gatagagaga taatcgccat cagcgcgaac atgcagaact atacagccgg tatcggtgaa
     1801 ttttatggcg ttactcaaca ggttggagat gacctgctgt aaacgcatcg ggtcgccatt
     1861 taaggccact ggcacatccg gttcaataaa gcagtacaag cctagctgct tgcgtaccac
     1921 cagcggtaaa tagttggcgg tgatgtggtt catcacttca cgcggtgaaa actcacgcgg
     1981 ttcgatcttc aactgttccg attcaatctt cgagaagtcg agaatatcgc tgataatttt
     2041 caacaacagg ctggaagagt tgttcattgc cgtcaccaga cgatcgacgc ctttcggtaa
     2101 ctctttggtt tgcaacagat ccaggttacc gataatgcca tacagcggcg ttcgcagctc
     2161 atgactgacg gtggcaagga acatcgattt tgactggctc gcctgttccg ctgcttgtgc
     2221 catctcctgc aacgactctt ccatcttcac gcgcgaagaa acatccacca gcacacaaat
     2281 ggccacgttt tcattacgat agcgcgaatg gacgaagctg atttgcaggt tggtattgtt
     2341 gctggtcagg acatcaacaa aattgacctg ctgcccacag ataatttgtg tcagccgttg
     2401 gcggtcctca tgcgtaagca tattgagata ggtatgcgcc agttcgttac ttaaaatatt
     2461 gacgccatca gcggtacgca aaatgcagat acccactggc gcggaggcga caatcttgcg
     2521 attaaactgc tcatgttctt ccagtcgcag ggcgtcgctt tccgccggaa tgaaaatacg
     2581 ccgctcgtac atccgcgcga gagtaaacaa tgcagctccg gcaagcacat tcagcaaaat
     2641 tgcgttaagg atcaacatgc gaatgcgttc cagcacctta tcaaccggca ccgaatacac
     2701 gatgcttagc gatgagggtg gcagattttt cttcagcacc agctcccgga acccttccgt
     2761 atagccaaac caggagcgtt cctgcatcca gcgaggatcg cccttaattt tgctttctgg
     2821 tccggtaagc gaaatcaggg tatgaccatt ttcatcaaga atggtaaccc ccatcggcaa
     2881 cgtaccaggt aagaaaaagt tctccatccg gatggtctgc tcgacaccca aaagcgcctg
     2941 caaccggttc gccagataaa ctggcgtcaa ggcgtaaaaa tacccgacgc cagggcgcgg
     3001 accttcgctg atccagtaga ggttactgcc actatcatct tgtggtgcat ttcgatattt
     3061 attaatgcgt tcatgcaaag cttttaacgc ggtatcgcgt tccactggca tatcacgcag
     3121 accgaaattg gccatgcaga ggttatcgct gccgattaaa aatacccggt tgagatcgta
     3181 agccgcagaa aaattatcgc gccagtagcg cataaaccac gccaatgact ccagagaacc
     3241 tcgccaggtg ttactcattg cggaacaatc agagtcagca aatagcggtt caaacgcagg
     3301 cacatccgtc tgcgtttctc gtccacgcgg ggaaagcaca ccgttttctg ccgataagcg
     3361 attttcagct atgtacttca gctctttcat cacatcagag gtgcgttgaa taaagcgctg
     3421 agcctgatcg gaactcagat taaattcctg acgaatttcc gattctcgct gatgtaacgc
     3481 attaacgatg taaaaaacgg atgaaaaagc aatcaacagc cagagcacta acgccaatgc
     3541 tctgaacatg tagcgcgagg ctttcagggt tgtacgaaaa gaagcaaggt atttcaaagg
     3601 ggcgaagctc cgcctcaggt gaccgatgga gtgtggttaa ggtagcggta aaagcgtgtt
     3661 accgcaatgt tctctcttct ctggaatatg atacaccgcc gagaaatcat caccttaacc
     3721 tctgataatc gtcatatacc ggacaagact agtggatttc agcatgcatt atatgaagtg
     3781 gatttatcca cgccgcttac gtaatcaaat gatcctgatg gcaatcctga tggtcattgt
     3841 cccaacgctt actattggtt atatcgtaga aacggaagga cgttcagcag tcttatctga
     3901 aaaagagaaa aaactttctg ccgtggtcaa cctgcttaat caggcgctag gcaatcgcta
     3961 cgatctctac atcgacttac cgcgtgagga gcgtatccgc gcattaaatg cagaacttgc
     4021 ccccattacc gaaaatatca ctcacgcctt ccctggcatc ggcgctggtt attacaacaa
     4081 aacgctggat gcgataatca cctacgcgcc ttcagcgcta tatcagaata atgtgggcgt
     4141 taccattgcc gcagatcacc ctggtcgcga agtcatgcgt acaaataccc ctttggttta
     4201 ttcaggcaga caggtgcgcg gcgatatttt gaattcaatg attcccattg agcgtaatgg
     4261 tgaaatcctc ggctatatct gggccaatga attaaccgaa gatattcgcc gccaggcctg
     4321 gaaaatggat gtgaggatta tcattgtgct caccgccggt ttgctgataa gcctgctgtt
     4381 gattgtcctt ttctcccgtc gcctgagcgc caatatagat atcatcaccg atggcctctc
     4441 gactctggct caaaatattc ccactcgatt accacaattg cccggtgaaa tggggcaaat
     4501 cagtcagagt gttaacaacc tcgcccaggc actgcgtgaa acgcgaacac ttaacgatct
     4561 gattattgaa aacgctgccg atggcgtcat tgccattgac cgccagggtg atgtaaccac
     4621 catgaaccca gcagcagaag tcatcactgg ctatcaacgt catgaactgg tagggcagcc
     4681 ttactccatg ttgttcgaca atactcagtt ctacagtccc gtactggata cgctggaaca
     4741 tggcaccgaa catgtagcac tggagatcag ttttccaggc cgtgaccgca ccattgaact
     4801 cagtgtcacg accagtcgta ttcataacac gcacggtgaa atgataggtg ctttggtgat
     4861 tttctctgat ttaactgccc gcaaagaaac ccagcgccgc atggcgcaag cagaacgcct
     4921 cgccacactg ggtgagctga tggctggcgt ggcgcatgaa gtacgtaatc cgctaacagc
     4981 tattcgtggt tatgtacaga tcttgcgcca acaaacccgt gacccaatac atcaggaata
     5041 tctgtccgta gtactcaaag aaatcgattc aattaacaaa gttattcagc aattgctcga
     5101 attttcacgc ccacgccaca gtcaatggca acaagtcagc ctcaatgcat tggttgaaga
     5161 aactctggta ctggtacaaa ccgccggcgt acaagcgcgg gtcgacttca taagcgaact
     5221 ggataatgaa ttaagcccga ttaacgccga tcgtgaactg ctcaaacagg tactactgaa
     5281 tatcctgatc aatgccgtcc aggctatcag tgcacgaggg aaaattcgca ttcgaacctg
     5341 gcaatacagc gactcacaac aggccatttc gatagaggac aacggctgtg gcattgatct
     5401 ctcgctgcaa aaaaagatct tcgatccctt tttcaccacc aaagcctcag gaaccgggct
     5461 tggtctggcg ttaagtcaac gcatcattaa tgcccatcag ggtgatattc gcgtcgccag
     5521 tttgccgggc tacggcgcaa ccttcacgct tattttaccg atcaacccgc agggaaatca
     5581 gactgtatga ctgctattaa tcgcatcctt attgtggatg atgaagataa tgttcgccgt
     5641 atgctgagca ccgcttttgc actacaaggt ttcgaaacac attgtgcgaa caacggacgc
     5701 acagcattac acctgtttgc tgatattcac cctgacgtgg tgttgatgga tatccgcatg
     5761 ccagagatgg acggcatcaa ggcactaaag gagatgcgca gtaatgagac ccggacaccc
     5821 gttattctga tgacggccta tgctgaagtg gaaaccgccg tcgaagcgct acgctgcggg
     5881 gccttcgact atgttattaa accgtttgat ctcgatgagt tgaatttaat cgttcagcgc
     5941 gctttacaac tccagtcaat gaaaaaagag atccgtcatc tgcaccaggc actgagcacc
     6001 agctggcaat gggggcacat tctcaccaac agcccggcga tgatggatat ctgcaaagac
     6061 accgccaaaa ttgccctttc tcaggccagc gtcttgatta gcggtgaaag cggcaccggg
     6121 aaagagttga ttgccagagc gattcactac aattcgcggc gggcaaaggg gccgttcatt
     6181 aaagtcaact gcgcggcgct gccggaatcg ttgctcgaaa gtgaactgtt tggtcatgaa
     6241 aaaggtgcat ttactggtgc acaaaccttg cgtcagggat tatttgaacg tgccaacgaa
     6301 ggtactctgc tcctcgacga aattggcgaa atgccgctcg tactgcaagc caaattacta
     6361 cgcattctgc aggaacggga atttgaacgg attggcggtc atcagaccat aaaagttgat
     6421 atccgcatca ttgctgccac caaccgtgac ttgcaggcaa tggtaaaaga aggcaccttc
     6481 cgtgaagatc tcttttatcg ccttaacgtt attcatttaa tactgccgcc tctgcgcgat
     6541 cgccgggaag atatttccct gttagctaat cactttttgc aaaaattcag tagtgagaat
     6601 cagcgcgata ttatcgacat cgatccgatg gcaatgtcgc tgcttaccgc ctggtcatgg
     6661 ccgggtaata ttcgagagct ttccaacgtc attgaacgcg ccgtcgtgat gaactcaggc
     6721 ccgatcattt tctctgagga tcttccgccg cagattcgtc agccagtctg taatgctggt
     6781 gaggcaaaaa cagcccctgt cggtgagcgt aatttaaaag aggaaattaa acgcgtcgaa
     6841 aaacgcatca ttatggaagt gctggaacaa caagaaggaa accgaacccg cactgcgtta
     6901 atgctgggca tcagtcgccg tgcattgatg tataaactcc aggaatacgg tatcgatccg
     6961 gcggatgtat aacgccaaaa cttgctatgc agaaatttgc acagtgcgca attttctgca
     7021 tagccgctca ttctccttat gaatccccat ctaatttatt cctttctatt caattagtta
     7081 aataacaaca ttcaataatc tcattctggc actccccttg ctattccctg actgtaccca
     7141 caacggtgta tgcaagaggg ataaaaaatg aaaacaaaat tgatgacatt acaagacgcc
     7201 accggcttct ttcgtgacgg catgaccatc atggtgggcg gatttatggg gattggcact
     7261 ccatcccgcc tggttgaagc attactggaa tctggtgttc gcgacctgac attgatagcc
     7321 aatgataccg cgtttgttga taccggcatc ggtccgctca tcgtcaatgg tcgagtccgc
     7381 aaagtgattg cttcacatat cggcaccaac ccggaaacag gtcggcgcat gatatctggt
     7441 gagatggacg tcgttctggt gccgcaaggt acgctaatcg agcaaattcg ctgtggcgga
     7501 gctggacttg gtggttttct caccccaact ggtgtcggca ccgtcgtaga ggaaggcaaa
     7561 cagacactga cactcgacgg taaaacctgg ctgctcgaac gcccgctgcg cgccgacctg
     7621 gcgctaattc gcgctcatcg ttgcgacaca cttggcaacc ttacctatca acttagcgcc
     7681 cgcaacttta accccctgat agcccttgcg gctgatatca cgctggtaga gccagatgaa
     7741 ctggtcgaaa ccggcgagct gcaacctgac catattgtca cccctggtgc cgttatcgac
     7801 cacatcatcg tttcacagga gagcaaataa tggatgcgaa acaacgtatt gcgcgccgtg
     7861 tggcgcaaga gcttcgtgat ggtgacatcg ttaacttagg gatcggttta cccacaatgg
     7921 tcgccaatta tttaccggag ggtattcata tcactctgca atcggaaaac ggcttcctcg
     7981 gtttaggccc ggtcacgaca gcgcatccag atctggtgaa cgctggcgga caaccgtgcg
     8041 gcattttacc cggcgcagcc atgtttgata gcgccatgtc atttgcgcta atccgcggcg
     8101 gtcatattga tgcctgcgtg ctcggcggtt tgcaagtaga cgaagaagca aacctcgcga
     8161 actgggtagt gcctgggaaa atggtgcccg gtatgggtgg cgcgatggat ctggtgaccg
     8221 ggtcacgcaa agtgatcatc gccatggaac attgcgccaa agatggttca gcaaaaattt
     8281 tgcgccgctg caccatgcca ctcactgcgc aacatgcggt gcatatgctg gttactgaac
     8341 tggctgtctt tcgttttatt gacggcaaaa tgtggctcac cgaaattgcc gacgggtgtg
     8401 atttagccac cgtgcgtgcc aaaacagaag ctcggtttga agttgccgcc gatctgaata
     8461 cgcaacgggg tgatttatga ttggtcgcat atcgcgtttt atgacgcgtt ttgtcagccg
     8521 gtggcttccc gatccactga tctttgccat gttgctgaca ttgctaacat tcgtgatcgc
     8581 gctttggtta acaccacaaa cgccgatcag catggtgaaa atgtggggtg atggtttctg
     8641 gaacttgctg gcgtttggta tgcagatggc gcttatcatc gttaccggtc atgcccttgc
     8701 cagctccgct ccggtgaaaa gtttgctgcg tactgccgcc tccgccgcaa agacgcccgt
     8761 acagggcgtc atgctggtta ctttcttcgg ttcagtcgct tgtgtcatca actggggatt
     8821 tggtttggtt gtcggtgcaa tgttcgcccg tgaagtcgcc cggcgagtac ccggttctga
     8881 ttatccgttg ctcattgcct gcgcctacat tggttttctc acctggggtg gcggtttctc
     8941 tggctcaatg cctctgttgg ctgcaacacc gggcaacccg gttgagcata tcgccgggct
     9001 gatcccggtg ggcgatactc tgttcagtgg ttttaatatt ttcatcactg tggcgttgat
     9061 tgtggtgatg ccatttatca cccgcatgat gatgccaaaa ccgtctgacg tggtgagtat
     9121 cgatccgaaa ctactcatgg aagaggctga ttttcaaaag cagctaccga aagatgcccc
     9181 accatccgag cgactggaag aaagccgcat tctgacgttg atcatcggcg cactcggtat
     9241 cgcttacctt gcgatgtact tcagcgaaca tggcttcaac atcaccatca ataccgtcaa
     9301 cctgatgttt atgattgcgg gtctgctgct acataaaacg ccaatggctt atatgcgtgc
     9361 tatcagcgcg gcagcacgca gtactgccgg tattctggtg caattcccct tctacgctgg
     9421 gatccaactg atgatggagc attccggtct gggcggactc attaccgaat tcttcatcaa
     9481 tgttgcgaac aaagacacct tcccgatcat gacctttttt agttctgcac tgattaactt
     9541 cgccgttccg tctggcggcg ggcactgggt tattcagggg ccgttcgtga tgcccgcagc
     9601 ccaggcgctg ggcgctgatc tcggtaaatc ggtaatggcg atcgcctacg gcgagcaatg
     9661 gatgaacatg gcacaaccgt tctgggcgct gccagcactg gcaatcgccg gactcggtgt
     9721 ccgcgacatc atgggctatt gcatcactgc cttgctcttc tccggcgtca ttttcgtcat
     9781 tggtttaacg ctgttctgac ggcaacccta cagacagaag gaatataaaa tgaaaaattg
     9841 tgtcatcgtc agtgcggtac gtactgctat cggtagtttt aacggttcac tcgcttccac
     9901 cagcgccatc gacctggggg cgacagtaat taaagccgcc attgaacgtg caaaaatcga
     9961 ttcactacac gttgatgaag tgattatggg taacgtgttg caagccggac tggggcaaaa
    10021 tccggcacgt caggcgctgc taaaaagcgg actggctgaa acggtgtgcg gattcacggt
    10081 caataaagtg tgtggttcgg gtcttaaaag tgtggcgctt gccgcccagg caattcaggc
    10141 aggtcaggcg cagagcattg tggcgggggg tatggaaaat atgagtttag cgccctactt
    10201 actcgatgca aaagcacgct ctggttatcg tcttggagac ggacaggttt atgacgtaat
    10261 cctgcgcgat ggtctgatgt gcgccaccca tggttatcat atggggatta ccgccgaaaa
    10321 cgtggctaaa gagtacggaa ttacccgtga aatgcaggat gaactggcgc tacattcaca
    10381 gcgtaaagcg gcagccgcaa ttgagtccgg tgcttttaca gccgaaatcg tcccggtaaa
    10441 tgttgtcact cggaagaaaa ccttcgtctt cagtcaagac gaattcccga aagcggattc
    10501 tacgactgaa gcgttaggcg cattgcgccc ggccttcgat aaagcaggaa cagtcaccgc
    10561 cgggaacgcg tctggtatta acgacggtgc tgccgctctg gtgattatgg aagaatctgc
    10621 ggcgctggca gcaggcctta cccccctggc tcgcattaaa agttatgcca gcggtggcgt
    10681 gccccccgca ttgatgggta tggggccagt acctgccacg aaaaaagcgt tacaactggc
    10741 ggggctgcaa ctggcggata ttgatctcat tgaggctaat gaagcatttg ctgcacagtt
    10801 ccttgccgtt gggaaaaccc tgggctttga ttctgagaaa gtgaatgtca acggcggggc
    10861 catcgcgctc gggcatccta tcggtgccag tggtgctcgt attctggtca cactattaca
    10921 tgccatgcag gcacgcgata aaacgctggg gctggcaaca ctatgcattg gtggcggcca
    10981 gggaattgcg atggtgattg aacggttgaa ttaatcaata aaaacacccg atagcgaaag
    11041 ttatcgggtg ttttcttgaa catcgacggc gaaggtaacc ccattaatca ccagtcaaaa
    11101 cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt ttcctgtttc
    11161 tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa ttccgtttcg
    11221 ctgcgtccgc catagtagtt gatatacacc tgataacgtc cgtgaatcgg cgctggcatg
    11281 gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc actgtttttc
    11341 agcaccgtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg gagatcgaga
    11401 tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat cgttcctgta
    11461 cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct gatgacctgc
    11521 acgctgttgc tgccttccgt gaaaatataa gggcgtgcaa atgaaccatc ggattcaatt
    11581 ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt acccgccgca
    11641 gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc tgccatgttg
    11701 acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcacc ctctgccgga
    11761 tgccagccag agagcggtgc atcaatctcg acttcctgca caccttcact gtgagcgaca
    11821 ggcgaaagcg ccaccagtaa caacggaaga aaaatctttc gcatagcatt agtccattaa
    11881 taattgtcgg gtaagcgttt cgatatagtt ctcatcaagc ccggtgggat aaccatcaaa
    11941 ggccaggtgc agatactcat gggttaaatc cagccgatcc tgaaacgtaa agaagttacg
    12001 aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta aacggcaaac
    12061 cgcaaacacg tctggttcgt tgtaccccgt ctcaccttgt aatatacgcc gccactgcgg
    12121 catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg agcgtggcgc
    12181 tccccagcgg ctaaggctat tgtcgggata agcaaacgcg agtatctgat cgtaacgctc
    12241 tccctgcccc gcctgggccg tggcttgccg ccaggaaagc gtcccttcgg tagcgcgact
    12301 gccgtgataa tgaaccggat cgcccgcata gataagatcc tgcgtccagg cggtcatcgt
    12361 tcgcgccccc gtggtagctg gcgaagcaga aacacgctgc gtggcgctgc tgtcagggat
    12421 ggtcaggcaa tcgccttccc gattggcgtt ttgctgtaaa aaagtgcgta tcgccacggt
    12481 catggctttc gctgcctcag gtggcgtact tttcgcctca cgatccagaa ctctggcaac
    12541 gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc tcagtaaggt
    12601 cgtctcaccg tggctgacaa acataatgtg gttaccattg gcaaacgtca cacgatagcg
    12661 cccattaagt acgccgggtt ttactgccgt cgaactcttt tccgccgtga ttttcttcag
    12721 cggatagcgg gcaaacagct ccaccaggac acattgccca ctggcaactt gcgtcggcac
    12781 tggcaaaacc cgattaagca ccgtcgcata acgggtcagt accgttttgc tggtgccact
    12841 gccagtcacc cacagcggtg taccatcggt taaccagccg gcaaaaccac cctgacggat
    12901 ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac tgccgagcat
    12961 ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt cctgagcttt
    13021 gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgag ttaaccgtta ccgaggtttc
    13081 tggcttcagc gtagtaagag acgctaacca cgcaggtgcc tgacgctgtt gccaatattg
    13141 cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc cacaggaacg
    13201 taccagcgcg gtatcgcgcg ttatactctc cccagcctga caacaataaa cctcttccgg
    13261 ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca gccaggcgta
    13321 aacatacagt ttccacaaac tccctaacgg cgtggtgagc gtgtcaggta atgccgagac
    13381 tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac cgcgcagcac
    13441 cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca atagccagac
    13501 gattctgcgc cagttcactt atttcacctg catcccggtc cattcgctcc ctgccgcaac
    13561 actttgctgt gcaggtgcat aggaacgcac ataacgagca ggaggcagaa cgaattgccc
    13621 tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt ctttcaccgg
    13681 caccatatag gccagtttgc ccatttcatt tcgcgctttt tccagcaact gcccctgctg
    13741 ttttccagcg ttcggtttat tgaccgaaat gccccacgta gtcctctcaa cgtcagcccc
    13801 tggcgggagc ggtacttcca cctgaccgta gcgcagaacg gcatcctgct cgctggtaag
    13861 cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca ccggctgcag
    13921 agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt caacggtcac
    13981 cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct gcacattttg
    14041 cggcgataat tcgtcaccaa aagagagaat gtccggcaca ccctgaccaa cccaacgcca
    14101 gtcttcgccc cctccagtta acttatgttt agcccatgcg cccgcaggcg caggcaaaac
    14161 aactggaggc attgtcgcca tatatttcgc cagccagttc atggccagcg cgcgctcaat
    14221 agtggattgc tcagcggtca aaccacttaa aatagcggcc gcctgggtag cgtcaccacc
    14281 gctattgagc agcacaaccg tgtgcgccag cggctgattg ctgtttgccg ctttatccca
    14341 cgcatattgc tgaacctgtg gcatcaccgt ggacttcaac tgcgcttttt tcagcaacgt
    14401 catggttaag acgtttgcca ccgcatctgc cagtggagat tccggtgtat cgaggatcag
    14461 gctgtcattg atatcgctgg tgtcttcctc agagaaatct tcagttttag ttccgcgccg
    14521 ggcgatagct tcatccaacc ctttcaacaa cgttttgcac ggtagattca tctcctgtgc
    14581 ccacgccagc accagcgcgc gatgcaatat cggcatgtta tctgcctgct cggcataact
    14641 gtcgagcata tgttgccagt aatccggttg ctgcgttacg ccgagcgcct ggctggcctg
    14701 ccagtcggcg taccatgccc atgccgtcag gaaggcgtcg ccattgccat cttcacccca
    14761 ccaggtaaag cgcgctccgg gccccgccag ttgcatcagc cgcagacggt tatcctgaat
    14821 catctgacga atgtcgttag cggcggcact ttgatgatcg gcaagtgaac gccaggcgag
    14881 actgagcggg atcagacggc taccggtgtt gatcacgccc ccccacggtt catcgactaa
    14941 cgcatcaaga ttgttacgga aaatctcctg cggcgtttcg ctactttgta gctggatatt
    15001 gctcgcctgc tcgggcaaca tcagcgcgtt atcgccaccg ccgagcatga cgtttttctg
    15061 ctgttcaacg ggccagctgt tatccacaaa agacagtttt gtgctaatgc tgtcctgtac
    15121 ttgcccattt tgttgcagtt ctgcacttaa caagccagat tgctgaatat tctgcgtcag
    15181 cgaaatataa ttcgcccctt tgtgcagcgt cagcgtctgg cgcatctcag cacctgcaaa
    15241 tttagtcacc agcgccaccg gttcgttatc ctgctgactg aagataaaca gtcctgctgc
    15301 cggtttgtcg cccatgcgat acaccgttgg catactccac ttcatgtaga gatttttttc
    15361 cgaacgcaga taagcacgcc cctgcccgac cagcccgtcg ccgttcatcc cacgcgcggt
    15421 gatgcgccag cgggttaacg aatcaggcat caggaacgtg aaatacgctt tgccttgttt
    15481 atcggttgtg agtgacggca tccatgccgc ggtatccacc tcttcacgcc gtggacgttc
    15541 aagcattttt actcgccgct cgctgcggtt agtcgcgcca ggcgcaaccg gctcgctgga
    15601 gagcgcctgg tcgtagctga taaacgacag actggagctg gtacgcacat tgttacgccc
    15661 cagcggatag aaaaatttgc cgatattcgg cgcgatttcc ggttgcagcg cgtagatcat
    15721 ttcatcgacc acaccaaccg ttagctgcgc agaaacaggt ttacctttca gcgacgaggt
    15781 taattcgaca ttgaccagtt caccaggctg gtaatgggtt ttttccgttt tcacccggat
    15841 atccagctgg ggaacggcaa ctttgatccc ggcgttctga aaactgtact gaccgttacg
    15901 ggtatacagc accgaaaaag tgatgttagg cgcaaaggaa ttgctcactg gtatgcgggc
    15961 ttcatactgg gtatcgttta aacgttgtaa cgtcagccag tttgccggat gcgatagcag
    16021 cgactgctgt tcaacgcgat cgcgttccag cgtcaataat gcttcatcaa ttggctccgg
    16081 gaaggtaatc agcatcttcg cggtttcgcc tggctggtac agcgttttat ccgccacgat
    16141 atctaccgta cccgtatgcg ccgtgctgcc cttaccgctg actgcatggc ttaacccggc
    16201 gagaattaag ccgtcttcat cgcgtaatgt cagattgtag ttgccaggtt tatcgaaatt
    16261 gacggtaaag gatttgccgc ctgacggtag atctccgcta tggctcgtgc ggtcttcgag
    16321 acgcaaccat tcatacgtaa caggaacctg ttttgaagat tccagcgcgg cataacggaa
    16381 cacaaccgac tcgccgctat tactgtattg tgcggcggta cttaaagagt aatgcgccag
    16441 gccgcgttca atgaggatct ctttggtggt ggtgacgcga tacgccgcgc cgtcactggc
    16501 ggagacggtt aacaaatagc ggctcggttt atcggcggcg gggagattta aagccacatg
    16561 accgctatca tcggacaccg tttcgctgcc ttccagcgac acgggaaaac gtccggcata
    16621 acgcaaatcg ttaccgacca ttgataattg ctgagcgcgc aaacttaact gcacgcgggc
    16681 atcttttacc ggctctccat ccgggtaaag cagttgcagt ttgccgctca ccgcttcgcc
    16741 tgttttgaac tcttttttgt cgagagctaa accaatctcg aaatgtggct tgatgtagtt
    16801 tgccacgcga aaactgctgc tatagacctg tttgcggtaa gccaggcgta actcataacc
    16861 tccggcgacg gcattttctg gcaggcggaa actcccctgc ccgccattac gcgcatcaag
    16921 cgtgacattg acggtttgca acagactgcc gttggcgtcc agcactgaaa gcttcgccgg
    16981 ggcgctgacg atgggggatg aatgcaacgg gtcgtggaac tcgcggccca tcactttgac
    17041 atcaacacga tcgcctgcac gatacagagg gcggtcggta aagatataca agcgggtgtt
    17101 gtagatttcg ctttcgtaga aaaaattctc ggagacaaaa acgccgcctt cagcatcttt
    17161 acccagaatg tatgaacgtt ccggcgatat atgttgtaac tgcaaggtac cgctgtcatc
    17221 ggtcacaccg cgggtcatca cgccaagacc gtcagtccac aagatctctg atcccggctt
    17281 cgcttccccc tgttttttac ccgcggtcca caccagaagc tcgttacctg acactttgct
    17341 aagcgccacg gtatcggaaa caaacaccac cgtcgtcgcc cgatacccac ctaccatcgc
    17401 ctcgacgagg tacagccccg gctcttgctt gcctagcgga atatagatgt tgcccggctg
    17461 cggcgagatg aaattgctgg atgcgccttc cagttttacc ccttgctgcg gctcgaccgg
    17521 tttagcctgc cataatgggt aacggaactg ttccaccagg ggatattttt tcagcggtga
    17581 aaactggttg ttctgtacat aacgggaagg tttaataatg gcattaccga gctgtaattc
    17641 gggtaacgcc tgagtcacat tctgccgaga ctgagaagaa aaagtacgct gcatcacgcg
    17701 gcgagatttg ccgtaccagt tatcccacag ccaggtcagc gtattgttca gcccgtcgcc
    17761 caaatattgc ggttgcacca caatgcgatg caggtttttc tgctggcgca aaaatgccat
    17821 cgggtcagga atacgataca ggcgaacgtc cacgccgccg tactcttcca tctgatagcg
    17881 tcgataatca cgccccggcg cttccagtcg cactttcgcc tcttcactgc tgctaaaact
    17941 gctgtcagcg agcaaaaaga atgttccccc ggcgggcggc gcatagttgc tggaaggaag
    18001 cgaatcatca gcattggcaa gccctgttcc cacaagagac aggaacaagc acgctgctat
    18061 cgcgccagaa aatttaaacg ataaatgcca atgaaattgg gattggaatc gttgggtatc
    18121 catcgggtgt ccttccatgt cataagttgt tgcagactga ctgcgcgcat tccgttgtca
    18181 gttttcgtgg cgcttccggt gtggtagatg acgtaacgcc ccatccagac cattaagtgc
    18241 tgggcatcgc cctgatcgaa aaaaatcata tcgccaggca gcgcctggtt tatgtcctgg
    18301 ccaataaatt ggctgttgta ctgaatcaaa ttaattgcgg tcacgtaggg gccggttttc
    18361 ccgttcccct gattccagtt ttgcgccagt tgccgctgtt caggtgttag cgtcatctct
    18421 ggcggcaaat actggttaga taacccgtta ctttttagcc atttactgtc gtgaactttc
    18481 agcgtctcgt tcgctgcaaa tcgcaccagg cccgcacaat cctgctgata ccagcgtgga
    18541 cttggaccct ggcggagctg ttcctgtgca atgcgtacaa accaggcgcg aaacagcccg
    18601 gattgttcga cattcagcat ttcgctatgg gtaacaacac aacacagcca gcaaatcagc
    18661 gccagcagcc cgtgtctcat agcggttgcc aggttatggg tagccactgc caggcggcac
    18721 cgggttccat ctgggccagc ttcatgacat aacgcggttg ttgagataaa gcgtccagct
    18781 tcggcattaa taaagtttgt gcggcgttat aaaaaaccgg ttcgagattc ttcggcaaac
    18841 tggtcagcgt ttcgttacgc agcagtttcg ccacgccttg tggattgata tagagcggaa
    18901 cgatgccgtc agtgggtatc acatccacca ttgccgggcg ggttttattc agtgtttgca
    18961 gcgcgttatt aaccagcgtg tcatcgaggg agaaaagcat cgtcttgttt tgcatcgcca
    19021 gcgacacgcg gaaaaaataa tccgacatta attgatcggg ttgcaccgcc tgcgctttcg
    19081 gatactggcc gtatcgggaa ctcacttcgc gacgccagat ttgcgcttcg ccctgctgag
    19141 tctggtttac cggcaaaaca ccttctggcg ctttgctttc gtgcgcacca atattttgcg
    19201 taaacagttt cccaggcagt tgtgcctgtt cggctgagcc atcaaattga ccaacaaata
    19261 gcggggtttg taattttgag tcttcatacc agcacagccc ggctgcacca tctaacgccc
    19321 cgttcagctt gtcgttttcc tggctgatgt gcgaaagcat ctcttcggca ataccatgcg
    19381 aatacggcac cgccacacag aagctggccc cggcaggcat actgttccat accggcgtaa
    19441 aatcgaaact ggcatctacg ctggcggatt catcatttaa cgccacaaag ctatgccagc
    19501 cgtcgttgct catttcgaag cgtacgccag caaaagaagg cattaatcgc tggtagccaa
    19561 accccagcaa cctggcgctg acaacaatac gctggcgtac tggcgttttt tcagcagcac
    19621 gctcttccag gccaaagctg gcttgccagc gttttttacc gctcaacaaa tcacttgcga
    19681 tcgccgtggc ttcggtatcc tgctggtcat ctttaaacag catatccgta ctggaaaaca
    19741 ccagcatctt gtcctgataa gtcgcgaaca tcagggcgtt attgccgtta tagcgcaact
    19801 gataaaccgg gatggtttca ctatttagct taatactact gatttccgtt ttgcttaact
    19861 ggctgtcgct ggtagcggca aacaacaatg gctccaacag tttgcttaaa ccactgcgct
    19921 ggatcagcac catgtaatgt gaaagatggc cttgtttatc gtgccatagc gccgcctgcg
    19981 cgggctgatc taagagtgac gaaaagagct tatctttcag cgtgagatcg tgttcataga
    20041 caatgcggcg aatgctgcct tcaatgccca gacgatcggc atgattctga taatagaaaa
    20101 cgaaatcttc actcagaaca tcgtggagaa acggaatggt aaggaggtct ttgggaagct
    20161 ggctcagaga gtcgctgtcg agaaagaggt ccggctcatt gagatcgatt tgcagattgt
    20221 tgtgcaccac cagcggcgac aacgtttttt ctggcccact gccagcatat tgcaacgccc
    20281 agacgccagc ggaaagcagt actattgcgc ccaaacctac aagaccatag aaccgccagc
    20341 ctttcgcctt tttttcacca ctcattgcca cattccttgt gtaaagccag ccatttttta
    20401 cgagcacagc aaaactttac cgcgccctaa tacgacaaaa gcccagactt tgcagcctgg
    20461 acttttcaat tcaaacaagg gagatagctc ccttttggca tgaagaagta aaattattct
    20521 tcttctggct cgtcgtcaac gtccacttcc ggagcgattt catcgtcccc ttccgcggca
    20581 ctgccatcga tggtatccag atcttcctcg tcaaccggtt cagcaacacg ttgcagaccc
    20641 actacgtttt catcttccgc agtacggatg aggatcacgc cctgggtgtt acggcctacg
    20701 atgctgattt ccgaaacgcg agtacgtacc agcgtaccgg catcggtgat catcatgatc
    20761 tggtcgcagt catctacctg caccgcgcca accactaaac cgttacgttc ggtaaccttg
    20821 atggagataa cccctttcgt cgcacgcgac ttagtcgggt attccgccac tgcggtacgt
    20881 ttaccataac cgttctgcgt tgcggtgagg attgcgccat cgccacgagg cacgatcaga
    20941 gagacgactt tatcgccttc gcctaaacga ataccgcgaa caccggtggt gttgcagccc
    21001 atcgcacgga cagaagactc tttaaagcgc accactttac cttcggcgga gaacagcatt
    21061 acttcgtctt cgccgctggt caggtcaacg ccgatcagct catcgccttc aaccagtttg
    21121 atcgccactt taccggcggt acgcagacgg ttgaactcgg tgaggacggt tttcttcaca
    21181 gtaccgttag cggtcgccat gaagactttc acgccttctt caaactcggt caccggcagg
    21241 atcgcagtga tacgttcgtc ctgctccagc ggcagcaggt tgacgatcgg acgaccacgc
    21301 gcgccacgag tggcttctgg taactggtag accttcatcg aatagacgcg gccacggctg
    21361 gagaagcaca gaatatggtc gtgggtgttc gccaccagca gtcggtcgat aaagtcttct
    21421 tctttaatac gtgcggcaga tttacctttc ccgccacgac gctgcgcttc atattcagaa
    21481 agcggctgat acttcacgta accctggtga gaaagcgtga ccaccacatc ttcctggatg
    21541 atcagatctt ccaggttgat gtcggcgctg ttagcggtga tttcagtacg acgtttgtca
    21601 ccgaactgtt cacgaaccag ttccagctct tcgcggatca cttccatcag acgatcggca
    21661 ctgccaagaa tacgcagcag ttctgcgatc tgatccagca gctctttgta ttcgtcgagc
    21721 agtttttcat gctcaaggcc ggtcagtttc tgcaaacgca gatccagaat cgcctgagct
    21781 tgctgttcgg tcaggtagta cagaccatca cgcacgccga actctggctc cagccattcc
    21841 ggacgcgcag catcatcgcc cgcacgttcg agcatggcgg caacgttgcc cagctgccac
    21901 ggattagcca ccagcgcagt tttcgcttct gcaggcgtcg gcgcatgacg gatcagttcg
    21961 atgatcgggt cgatgttcgc cagcgctacg gctaatgctt caaggatatg agcacgatcg
    22021 cgagctttac gcagttcgaa aatagtacga cgagtcacca cttcgcggcg gtgacgaaca
    22081 aacgccgcga tgatgtcttt caggttcatg atcttcggct gaccatggtg caatgccacc
    22141 atgttgatac cgaaagaaac ctgcaactgg gtctgggagt agaggttgtt gagcacaact
    22201 tcaccgaccg catcgcgttt cacttcaatc acgatgcgca taccgtcttt gtcagactcg
    22261 tcacgcagcg cgctgatgcc ttccacgcgt ttttctttta ccagttccgc aatcttctcg
    22321 atcaggcgcg ctttgtttac ctgatacgga atttcgtgga cgataatggt ttcacgtccg
    22381 gttttggcgt caacttccac ttcagcacgg gcgcggatat ataccttgcc gcgaccggta
    22441 cggtaagctt cttcaatacc gcgacgaccg ttaatgattg ccgccgtcgg gaagtccggc
    22501 ccggggatgt gttccatcag cccttcaatg ctgatgtctt catcatcgat atacgccaga
    22561 caaccgttga tgacttccgt caggttgtgc ggcgggatgt tggttgccat acctacggcg
    22621 ataccggaag aaccgttcac cagcaggtta ggaattttgg ttggcatgac gtcgggaatt
    22681 ttttccgtgc cgtcatagtt atctacgaaa tcgaccgtct ctttttcgag atcggccatc
    22741 agttcatggg caattttcgc cagacggatt tccgtataac gcattgccgc cgcagagtcg
    22801 ccgtcgatgg aaccgaagtt accctgaccg tctaccagca tgtaacgcag cgagaatggc
    22861 tgcgccatac ggacgatcgt gtcataaacc gccgagtcac catggggatg gtatttaccg
    22921 attacgtcac caacgacacg ggcagatttt ttataggctt tgttccagtc attgcctagt
    22981 acgttcatgg cgtaaagtac gcgacggtgt accggcttca ggccatctcg gacatctggc
    23041 agcgcacggc caacaatgac cgacatcgca taatccagat aggagctctt cagctcttcc
    23101 tcaatgttga ccggtgtaat ttctctcgca aggtcgctca tctaaccgct atccctctac
    23161 tgtatcccgg attcaaaggt cgcaaattat aacacagccg cgcagtttga ggtaaaccta
    23221 tacgctttat tcacctccaa tgcctgatat actcgtttgt cttgccaatt acggagtaga
    23281 agtgccaatg aatgccgaaa aatcgccggt aaaccataac gtagaccacg aagagatcgc
    23341 taaatttgaa gctgtcgcct cccgctggtg ggatcttgaa ggtgagttca aaccgctgca
    23401 ccgcattaac ccgctgcgtc tgggctatat tgccgagcgt gctggcggtt tatttggcaa
    23461 aaaggtgctc gatgtcggtt gtggcggcgg cattctggcc gagagtatgg cgcgcgaagg
    23521 cgcgacggtg accggtctgg atatgggctt tgagccattg caggtggcaa agttgcacgc
    23581 gctggaaagc ggcattcagg tagagtacgt acaggaaacc gtggaagagc acgcggcaaa
    23641 acatgccggg cagtatgatg tagtgacctg catggagatg ctggagcacg tccccgatcc
    23701 gcagtcagtg gtcagagcct gtgcgcaact ggtgaaacca ggcggcgatg tctttttctc
    23761 gacgcttaac cgcaacggca agtcatggct gatggcggtg gttggtgctg aatatatttt
    23821 gcgcatggtg cccaaaggca cgcatgatgt gaagaagttt attaaaccgg cagaattgct
    23881 gggctgggtg gatcagacca gtttaaaaga gcggcatatg accgggctgc attacaaccc
    23941 gatcactaat tcctttaagc tcggccccgg cgtggatgtg aactatatgc tgcacacgca
    24001 gaataagtga ggttgttgtt tggattcatc cgacaaccgt gccaatgcct gatgcgacgc
    24061 ttgccgcgtc ttatcaggcc tacaaatgct cccgtaggcc agataaggcg tttacgccgc
    24121 atccggcaaa agtgccggtc agacagcgat taccatttca ccgtcatcga caaaaaccct
    24181 gccgtctggg caaaatcatc actccctttc tgccacgcca cgctgccgcg cagggacact
    24241 cgctgactga tattgcccgt gactcccact tttatttcac cccgttgctt caccccatcg
    24301 tcactgatag tgctgccatc ctcttcaatt tccgtcgcat ggggatcgtg ataataattc
    24361 agatctaacg ttggcgtgac accaacagca gtactccatt cgctgtgtaa acccagccgc
    24421 gtctgaatat catcaccctg cgattgtgac acgcgcgcat ggttagcggc ggtaaaatca
    24481 tcctgctgca cgccctgata aatcacctgc gtctgcggtt caatcaccac accatgcccc
    24541 ggtaaccact gataaccggc ttccagcgag gcgataatcc ccgacgagtg gtaatgatcc
    24601 tcgccatcgt cttgttcgga aacatcattg ttaaaccacg catattgcag ccagctatcc
    24661 agccagaccc cctgcttctg attaccgtgc tgataccagc ttgaggtcag cccaacggca
    24721 taaccgtggt tctggttatc ggcgcgagtt ccggtcatat tcgagcggct gtcgccctgg
    24781 ttatcgctgt agccaccaac aatcccaagc atccactcgc catcatcacc ccagtgcccg
    24841 ctaaacaggt tgccgctaag ctgcaccgta gaagtgtctt catgctgagc caactgccct
    24901 gccgccgtgt aatgataacg ccctccgata acgcgtaaat tcagcgtctg accatcgcca
    24961 ccagcctgat cgcgtcgctc catcacaaat gcctgatttg ccgcacgtag gttattaaaa
    25021 tatccaccaa ctttggcatt caacaccggc tggtaagcag gcgtaggtcc agggtcgggg
    25081 gttgggtcgg gatccggcgt gggatcagga tctggagtcg ggtccggatc gggcggcggc
    25141 gtcggagtga cttcttgcga tcgcagatac cagtcgttgt tatcttccac cagcgtatag
    25201 tcatacgccc ccatattgac gtagccgctt cctgccagac ggaactgcgc attgttttga
    25261 aactgcgtgg gatcagctgc gaaatcaacc actttaatac ctgtagatgt cggctcacca
    25321 atacccgtaa tggggttaat taccacggcc gtgttgccag cagtattacc gttcaatacc
    25381 agttggtcac ttgccgagtc atcgccgttt aattcgctgt cgagcagtag cgtaccgccg
    25441 ccggtataat caccatttac cgtcagcgtg tcgccagcga cgccattttg caggctaatc
    25501 gcaccggacg tattggtcag attgccatta accgtcacgt cggaaagata agtcaggctg
    25561 tcagtttcaa ggttggtatt aagctcgcct gtcagcgtca cgccatcggt ggcattcacc
    25621 aacgtaccgt cgccgctaaa cagcgaggca tccagcacct ggttcgtatc ctgctgcgtc
    25681 aatcgcaaca cagtaccatc ggttatgtcg atatcgccgc cagaggtggc atcgattgag
    25741 cgaatatcct gattttgccc ggtttcaaaa acaccgtctt cccccaccca cagtgaagaa
    25801 acataaggaa taatattctc cgcttcacct tttagcgttc cgtcctccac acgtaccgca
    25861 gacgtcgcag tgccgctggc ggtcaactcc agcgtaccgc cgccactttt agtcaacgtg
    25921 cttacgctct ccagttgttg cccggtacta tccgccatca acccgcccca ttgcgtatct
    25981 acgccatcct caacgtgtat ttcgccatca gcgcgcattt caatattcct gccctgacct
    26041 gttgtagccg agcgagtgtc aacatcagtc gtcacatcca tttcactgtg ttgttgggga
    26101 tctgtgaaga tcactgaacg gttgtaactt gtgtccccca attgcgaatt atcgctgact
    26161 tcaagcgtac cggaggcgat ttgcgtatta ccgagataac cgttgttatt cgccagcgtt
    26221 acgcgctgat cccccgcatt aatgacgaca taaacgccat tctggctggc atcactgccg
    26281 ccaatatgta aatcatcgcc actgctgata tcaacgacat cattactgcc aatcactgtc
    26341 cccccgctgc cgctgatttg cagaccgtca ttgtatgagc tatcgccatc ccaggtggtg
    26401 aaatcagaga gatccaatac tccgccattt agcacgatcg attgcggatc gctctgcatt
    26461 gcccgtaaat cggcctgatt ccccgctagc gacaatacgg cgttatcttc caccacaata
    26521 tcgccggtta atgccatcga ctgcgcccct gtcaggagat agctgccatc ttgcgctacc
    26581 gtcaactgtc cttcaccctg aatcgagccg gaaaaaccgc cctggtttac ggtaacattg
    26641 ccgcctgcgt cgatattcaa aatgccatta gcaaaaccgg ttaatgagtg cacaaacgtt
    26701 tgttgtgtat tgccaacatt cagttctgcc tgatactcag aatgaacagt gctgccgacc
    26761 atcagaccaa agcaatcctg cggatcgctc tgacagtgcg tgtcgccgac attcattaac
    26821 tcatcgctgc gccccaacgt cacctcgcca ttctggatac ttatctcgcc ggtaaacgca
    26881 ttattgtcgg cattgaggac taactctccc gctccctctt taacgatgac tcccgttccg
    26941 gcaagagaat caatcgcgcc atcgttgctg gtattgccta ttaccagcgt tttatcggcg
    27001 gcaatgttaa attcagccac actatgaccg atatacataa agccccccgc cgctgccgtc
    27061 gccgaatggt catatcccga ggcgctgtta ttttcatcaa tcatcacacc attgttttgg
    27121 tcataattat catcaacaga aatatcaata agatacgggg atgtggtgct gctggtataa
    27181 attgctccgc cata
//
LOCUS       DAZUCC010000048        27069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZUCC010000048 DAZUCC010000000
VERSION     DAZUCC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.048"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..79
                     /gene="ymgJ"
                     /locus_tag="ACHY09_001753"
     CDS             <1..79
                     /gene="ymgJ"
                     /locus_tag="ACHY09_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFS3884735.1"
                     /translation="FYPTTLQRTAFKKCLFLMRDIIISS"
     gene            194..538
                     /locus_tag="ACHY09_001754"
                     /pseudo
     CDS             194..538
                     /locus_tag="ACHY09_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(903..1169)
                     /gene="minE"
                     /locus_tag="ACHY09_001755"
     CDS             complement(903..1169)
                     /gene="minE"
                     /locus_tag="ACHY09_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFS3884736.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1173..1985)
                     /gene="minD"
                     /locus_tag="ACHY09_001756"
     CDS             complement(1173..1985)
                     /gene="minD"
                     /locus_tag="ACHY09_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFS3884737.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2009..2704)
                     /gene="minC"
                     /locus_tag="ACHY09_001757"
     CDS             complement(2009..2704)
                     /gene="minC"
                     /locus_tag="ACHY09_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFS3884738.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3223..3591
                     /gene="ycgJ"
                     /locus_tag="ACHY09_001758"
     CDS             3223..3591
                     /gene="ycgJ"
                     /locus_tag="ACHY09_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFS3884739.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3694..4095)
                     /gene="pliG"
                     /locus_tag="ACHY09_001759"
     CDS             complement(3694..4095)
                     /gene="pliG"
                     /locus_tag="ACHY09_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFS3884740.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4337..4630
                     /locus_tag="ACHY09_001760"
     CDS             4337..4630
                     /locus_tag="ACHY09_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFS3884741.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4702..5361
                     /gene="ycgM"
                     /locus_tag="ACHY09_001761"
     CDS             4702..5361
                     /gene="ycgM"
                     /locus_tag="ACHY09_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFS3884742.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5453..5899
                     /gene="ycgN"
                     /locus_tag="ACHY09_001762"
     CDS             5453..5899
                     /gene="ycgN"
                     /locus_tag="ACHY09_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFS3884743.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6106..6432)
                     /gene="hlyE"
                     /locus_tag="ACHY09_001763"
                     /pseudo
     CDS             complement(6106..6432)
                     /gene="hlyE"
                     /locus_tag="ACHY09_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            6693..7112
                     /gene="umuD"
                     /locus_tag="ACHY09_001764"
     CDS             6693..7112
                     /gene="umuD"
                     /locus_tag="ACHY09_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFS3884744.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            7112..8380
                     /gene="umuC"
                     /locus_tag="ACHY09_001765"
     CDS             7112..8380
                     /gene="umuC"
                     /locus_tag="ACHY09_001765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFS3884745.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(8425..8955)
                     /gene="dsbB"
                     /locus_tag="ACHY09_001766"
     CDS             complement(8425..8955)
                     /gene="dsbB"
                     /locus_tag="ACHY09_001766"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFS3884746.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9101..10642)
                     /gene="nhaB"
                     /locus_tag="ACHY09_001767"
     CDS             complement(9101..10642)
                     /gene="nhaB"
                     /locus_tag="ACHY09_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFS3884747.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            10864..11583
                     /gene="fadR"
                     /locus_tag="ACHY09_001768"
     CDS             10864..11583
                     /gene="fadR"
                     /locus_tag="ACHY09_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFS3884748.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(11635..13167)
                     /gene="ycgB"
                     /locus_tag="ACHY09_001769"
     CDS             complement(11635..13167)
                     /gene="ycgB"
                     /locus_tag="ACHY09_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFS3884749.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            13497..14795
                     /gene="dadA"
                     /locus_tag="ACHY09_001770"
     CDS             13497..14795
                     /gene="dadA"
                     /locus_tag="ACHY09_001770"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFS3884750.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            14805..15875
                     /gene="dadX"
                     /locus_tag="ACHY09_001771"
     CDS             14805..15875
                     /gene="dadX"
                     /locus_tag="ACHY09_001771"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFS3884751.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(15932..17668)
                     /gene="cvrA"
                     /locus_tag="ACHY09_001772"
     CDS             complement(15932..17668)
                     /gene="cvrA"
                     /locus_tag="ACHY09_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFS3884752.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17763..18677)
                     /gene="ldcA"
                     /locus_tag="ACHY09_001773"
     CDS             complement(17763..18677)
                     /gene="ldcA"
                     /locus_tag="ACHY09_001773"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFS3884753.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            18777..19388
                     /gene="emtA"
                     /locus_tag="ACHY09_001774"
     CDS             18777..19388
                     /gene="emtA"
                     /locus_tag="ACHY09_001774"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFS3884754.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(19390..20124)
                     /gene="ycgR"
                     /locus_tag="ACHY09_001775"
     CDS             complement(19390..20124)
                     /gene="ycgR"
                     /locus_tag="ACHY09_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFS3884755.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            20325..20579
                     /gene="ymgE"
                     /locus_tag="ACHY09_001776"
     CDS             20325..20579
                     /gene="ymgE"
                     /locus_tag="ACHY09_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS3884756.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(20629..22599)
                     /locus_tag="ACHY09_001777"
     CDS             complement(20629..22599)
                     /locus_tag="ACHY09_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFS3884757.1"
                     /translation="MRLKKRYLCTALSLAFTQQALAAQESDTLTVWSSPVSSTTTTVL
                     DHPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASNDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(22625..23479)
                     /gene="modD"
                     /locus_tag="ACHY09_001778"
     CDS             complement(22625..23479)
                     /gene="modD"
                     /locus_tag="ACHY09_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFS3884758.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     NPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            complement(23476..24288)
                     /locus_tag="ACHY09_001779"
     CDS             complement(23476..24288)
                     /locus_tag="ACHY09_001779"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS3884759.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVVELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            complement(24298..25056)
                     /locus_tag="ACHY09_001780"
     CDS             complement(24298..25056)
                     /locus_tag="ACHY09_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3884760.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGQ
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(25053..26033)
                     /locus_tag="ACHY09_001781"
     CDS             complement(25053..26033)
                     /locus_tag="ACHY09_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS3884761.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVTV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(26033..27055)
                     /locus_tag="ACHY09_001782"
     CDS             complement(26033..27055)
                     /locus_tag="ACHY09_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFS3884762.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
BASE COUNT         6749 a         6681 c         7011 g         6628 t
ORIGIN      
        1 tttttatcca actacacttc agcgcactgc gtttaaaaaa tgcctctttc ttatgcggga
       61 tatcattatt tcatcatgat gtctttggtg agcggtgaac acaatacact tgcgctgcct
      121 attcaggagc aatcccctgg tctggtgcct gcgggatgat gttgcagcag agctaaggtc
      181 acttagacgc tactatcatt tatccaatgg catggggtcg aaatcagtcg atacccgcag
      241 tatatatcgt gaacttggtg caacgctgag ttacaacatg cgtctgggga acggcatgga
      301 agttgagccg tggctgaagg cggctgtgcg caaagaattt gtcgatgata accgggtgaa
      361 agtgaataat gacggtaatt ttgtcaatga tttgtcgggc agacgtggaa tatatcaggc
      421 aggtattaaa gcctcattca gcagttcgtt aagcgggcat ctcggggtgg ggtatagcca
      481 tggtgccggt gtggaatccc catggaacgc ggtggctggt gcgaactggt cgttctgacc
      541 atcaacgatt aaactgcgct tcgcgcagtt ttcgtttacc gggtgctaaa agggaaaatt
      601 ctgcggcaaa aaaagcccgc cagttacggc gggaaacctc atcctatggg agaacaatga
      661 ataatgaaat tgcggggtta tcatctccca gtatacccat actaacaata aggttattta
      721 ctcagccagg tataaacaat ttgttctgtg tggggggaac ggcattaaga tgtaaagggg
      781 gggaagcaat gagtagcatc agcaagaata ctcgccgctc ttatcaaacg tggatgaggg
      841 ggatgaaaaa ctcaaggcag agatgactct gccttgaaga taaatgcgct tttacagcgg
      901 gcttatttca gctcttctgc ttccggtaag gtcacgttca gctcaagaat agaaatatcg
      961 ccatcttttt gctcaagctg tacggttacc atctcaggat caatttgtac gtatttacaa
     1021 atgacctcaa gaatatcttt acgcaactgc ggcagataat gcggttctgc atcactgcga
     1081 cggcgttcag caacaataat ctgcagccgt tcttttgcaa tgttggccgt gtttttcttc
     1141 cgcgatagaa agaaatcgag taatgccata acttatcctc cgaacaagcg tttgaggaag
     1201 cctttcttct cttcttcaat gaagcggaaa ggacgttctt ctcccaacag acgttctacg
     1261 gtatctgcgt aggctttacc cgcatcggcg ttaatgtcga gaatgaccgg ttcaccctgg
     1321 ttagaggcgc gcaatactga ttgatcctca gggatcacgc cgacgagttt gatgcgcagg
     1381 atctccagca catcttccat gctcagcatg tcacctctgc ttacgcggcc tgggttatag
     1441 cgcgttaaca gcaggtgctc tttaataggc tcttcgccat tttctgcgcg gcgtgatttc
     1501 gacgccagaa tgcctaaaat acggtcagag tcgcgtactg aggagacttc cgggttggtg
     1561 gtaataatgg cttcgtctgc aaaatagagt gccattagcg caccggtctc aatccctgcc
     1621 ggggagtcgc aaacaataaa ttcaaagtcc atcgctttca gatcatcaag aactttggcg
     1681 accccttcac gggtgagggc atctttatcg cgagtttgcg atgccggaag aatatagaga
     1741 ttttcagtac gcttatcttt aattaacgcc tgatttagcg ttgcatcgcc ctgaatgacg
     1801 ttgacgaaat cgtaaacgac ccgacgttca caacccataa tcaggtcgag attacgcagg
     1861 ccgatatcaa aatctatcac gacagttttc tttcccttct gggccaaacc agtggcgatg
     1921 gccgcgctgg aggttgtctt accaacaccc cctttgcccg aagtaacaac aataatgcgt
     1981 gccatagaaa ttccttgtta aaaagggatc aatttaacgg ttgaacggtc aaagcgtttt
     2041 cgactaactg cagtcgcgcc gctttgccat aaaattctgc tgggatttga tcactcagcc
     2101 agtattcacc tgcgatggac accagttccg ccatcaggtt cgtacaaaat atttgcgttt
     2161 cccggtcacc acttgcccct gccagcgcac gaccgcgcat catgccatag acgtgaatat
     2221 taccatcggc aatcaattcg gccccagcgc taacgtggct tgtaacaatc agatcacatt
     2281 gtggagcata aatacgctga ccggaacgca ccggggtatc tattaaacgc gtttttgtga
     2341 ccggcgttgt attttgcgct ggagcctgcg gtgcgggagc tggacgcggc gctttttcct
     2401 ttccttccgt caggataggc agccccatct tttcaatttc ggctttaagt tgcgcatctt
     2461 tgcagccact tacgccaata acccgcaaac cggttgccga aaccgcctta tgcatcgctg
     2521 accagtttac cgggtcttcc agtgcactga cgttgagtac aacgggggca tgttttaaaa
     2581 atgcgggggc ctgagcgatt ttgtcttcca gcgcctgatg gataacctta ggttctgcct
     2641 catgcagatg aaccacagat aaagtgaagc tactgccttt aagctcgatt ggcgtgtttg
     2701 acatcctggc cttactcaat tagctattaa tcatcgccag cgcgcgatga tgttccgaag
     2761 actataaggc atgttatagt ctggattata ttgaggcaag tcaccctccc atttattcag
     2821 agtaaaagtc tattctgtga taaatggcgc tgattcatag cttaaaaaat acccttgtca
     2881 atcaacccat tgccgtcgta cttttgattg ttcttattta cgcttccttt ttccgcaccc
     2941 taatcgtgac agcatcttca gacctgttta aatatccagt attgaggcga gtgtattttt
     3001 tcaccacgcc aggctttctt aactttccat ttttgcagca tcattaaaga acagataccc
     3061 gttatcttaa tatccgccac agaattgctc tatcatagaa accgcagaag acgtctgata
     3121 cttctgtcag gtcatcgtaa ctcgtccagg tcagcggttt tgattacgga ttgatgatga
     3181 aatgagcata agtgttgtac ggttggatta tcaggatgtg ctatgaatat gatgcgtatt
     3241 ttttatatcg gattgtcagg tgtggggatg atgttctcat caatggcgtc tggacatgac
     3301 gctggcggac ttcaatctcc ggcgtgcggc gttgtttgcg acccgtatat ttgtgttaac
     3361 tcagatggca tttccccaga gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac
     3421 ttacaatcat tacaaggcta cgatcccagc gaatttacat tcgctaacgg tgtattttgc
     3481 gatgttaaag aaaaattatg tcgtgatgat cgttattttg gtgtgaatgg taagcggagt
     3541 ggaaaaatca atcaaaccac cacaaagatg ttatttatgt gtcgtgagtg atgtcttgtt
     3601 tacattatgt tctattcgtc attaacctct tgagcgacag agaggagtgg ggcgttaacg
     3661 tttatgacgg ggagagtccc cgtcggttga tatttactta atttgaatat cgacgttata
     3721 ttttttagtt ttgtttttac gggcgtcgtt tcgggtttgc agcactcgca actcatattt
     3781 gccagaagca gggagtgagt attgtccgtg actatcgagt tctggggaat atctggataa
     3841 atcaacagaa tcatcgattc ctggcccgaa gaggtaagta tcagcgcctt cattagagat
     3901 actcacatgc actttctgac cttttttggc atagaaggta tatgtatcgt aatcgtatcc
     3961 ctttatttcg ccggagtact gagcgctgct atggcctttt ctgaactcaa cattgacatt
     4021 cttacccgca gcaaagctcg tggaggttag cagtgcaagc aacaatacag ccttactgat
     4081 gcccttaatt ttcattggta gtctcctgtt cagaatgagt acgatgaact gttataatat
     4141 aacaattcat cacaggagaa ttccagtggc tttcctgcgt tcggttaaat cccggcgcgg
     4201 catttttgcc ggggggatta cttataaagg gggcatggac tgaacaaacc gtaggaatct
     4261 acagctggct ggcttgtggc gcgggtttca ttgatgttag cttatgccaa aacctggcat
     4321 cttaaagagt aaaagtatgt tttgtgtgat ttatcgaagc agcaagcgtg atcagaccta
     4381 tttatatgtc gaaaaaaaag acgatttttc gcgtgttcct gaggaactga tgaaaggttt
     4441 tggtcagcct cagttagcga tgattcttcc gctggatggg cggaagaaac tggtcaatgc
     4501 cgatattgaa aaggttaaac aggcattaac cgagcaaggt tactatttgc agttaccgcc
     4561 accacccgaa gatttgctga agcaacatct ttccgtcatg gggcagaaaa cagacgacac
     4621 gaacaaataa ccgatatccg gcggtggcat tatctttgtc ggcgcgggtt ttcatatcca
     4681 cgataaggtg aggggaacgt tatgtatcaa catcacaact ggcaaggtgc gctgctggat
     4741 tatccggtga gtaaagtagt ctgtgttggt agtaactatg ccaaacatat taaagagatg
     4801 ggcagcgcag tgcccgaaga gccagtgctg tttattaaac cagaaacggc actgtgcgat
     4861 ctgcggcagc cattggcgat cccatccgat ttcggttcag ttcatcatga agtcgaactg
     4921 gcggtattga ttggcgcgac gctgcgtcag gctacggaag agcatgtccg taaagccatt
     4981 gccggttacg gcgtggcgct cgatctgaca ttgcgtgatg ttcagggaaa aatgaagaaa
     5041 gccgggcagc cgtgggaaaa ggccaaagcg tttgataact cttgtccgct ttccgggttt
     5101 attcccgcgg cggaattcac cggcgatccg caaaatacaa cgctgggcct gagcgtaaac
     5161 ggcgaacaac gccagcaagg tacgactgcg gacatgatcc ataaaatcgt tccgctgatc
     5221 gcttatatga gcaagttctt taccctcaag gccggtgacg ttgtgctgac aggcacgcct
     5281 gatggcgtcg gcccgttgca aagtggtgat gagctgacag tcactttcga tgggcattct
     5341 ttgacaactc gcgttttgta atactttttg ccgcctgaaa gcggcggcaa aacttgcatc
     5401 gctgtgccag actggttata aggtgcgttt taacgtaatg gcggaacacc tgatgagcga
     5461 tgtacctttc tggcaaagta aaaccctgga cgaaatgagc gatgcggaat gggagtcgtt
     5521 gtgtgatggt tgcggtcagt gttgcctgca taagctgatg gatgaagaca ccgacgaaat
     5581 ctacttcact aacgtcgcct gtcgccagct caatattaaa acctgtcaat gtcggaacta
     5641 cgaacgtcgt ttcgagtttg aacctgactg cattaaatta acccgtgaaa atctgccaac
     5701 attcgaatgg ctgccaatga cctgcgctta tcgcttgctg gcggaaggta aagatttacc
     5761 tgcgtggcat ccgctgctta ctggttcgaa agcggcaatg catggtgaac gtatctctgt
     5821 gcgtcatatc gcagtgaaag aatcagaagt gattgactgg caggatcata tcttaaataa
     5881 acctgactgg gcgcagtgat aaatttaata gatgttgatg ggggctttct taatatacgt
     5941 ctggatactc gccatttcgt agccctgttt ttgaaagcgc tctgtcataa tgagagttac
     6001 ccgatacctt attatcaaca ggtaactctc aatctttaaa aaacggctgt gtttttaaat
     6061 gtgataaacc ttataccttg agtacatgga gagaataatc gcttatcaga cttcaggtac
     6121 ctcaaagagt gtctttttac cgtgtctttt ctgatactca ttacaggtgt taatcatttt
     6181 tttggccgct tcttttagca aagaaagcat taaatcatca taatcaacat agaatctggt
     6241 tgtttcagtt tccgttttta tctccccgat ggcggctatt tcggtggtta atttcaattt
     6301 ggcggcatcg atatctttat tcgcttgttt aaccgtgtta gacagggtgg taaagaaact
     6361 ctgcacagat tttaacttgt tcttcaattc tggaatcagt ttcccttcaa ctacgcccgc
     6421 tgcaacagaa tacagtttct tgatgccgtc atccagtacc ttaatgagaa tgcctttctg
     6481 ggcgaatgct tctattctat tttttagccg ggcgatattt ttcatttctg ctggatgagc
     6541 gtcgtcgcca gaaggccacg tgagcacagg ataagagaac gaaaaatcag cagtctatgc
     6601 agcgacaaat attgatagcc tgaatcagta ttgatctgct ggcaagaaca gactactgta
     6661 tataaaaaca gtataacttc aggcagatta ttatgttgtt tatcaagcct gcggatctcc
     6721 gcgaaattgt gacttttccg ctatttagcg atcttgttca gtgtggcttt ccttcaccgg
     6781 cagcagatta cgttgaacag cgcatcgatc tgaatcaact gttgatccag catcccagcg
     6841 cgacttactt cgtcaaagca agtggtgatt ctatgattga tggtggaatt agtgacggtg
     6901 atttactgat tgtcgatagc gctattaccg ccagccatgg tgatattgtc atcgctgctg
     6961 ttgacggcga gtttacggtg aaaaaattgc aactacgccc gacagtgcag cttattccca
     7021 tgaacagtgc gtactcgccc attaccatca gtagcgaaga tacgctggat gtctttggtg
     7081 tggtgatcca cgtcgttaag gcgatgcgct gatgtttgcc ctctgtgatg taaacgcgtt
     7141 ttatgccagc tgtgagacgg tgtttcgccc tgatttatgg ggtaaaccgg tggttgtgct
     7201 atcgaataat gacggttgcg ttatcgcccg aaacgctgag gcaaaggcgc tgggcgttaa
     7261 aatgggcgat ccctggttca aacaaaaaga tctgtttcgt cgctgtggcg tggtttgctt
     7321 tagcagcaat tatgagcttt acgcagacat gagtaatcgg gtgatgtcga cgctggaaga
     7381 actatcgccc cgcgtcgaga tttacagtat tgatgaggca ttctgcgatc tgaccggtgt
     7441 gcataattgt cgcgatctga ctgattttgg cagagaaatt cgcgcaacgg tgctacaacg
     7501 tacccatctt actgttggtg tggggatcgc ccagaccaaa acgctggcta agcttgccaa
     7561 tcatgcggca aaaaaatggc agcggcagac gggcggggtg gtggatttat caaatctgga
     7621 acgccagcgt aaattaatgt ctgctcttcc cgtggatgaa gtctggggga ttggacggcg
     7681 gatcagcaaa aaactggacg cgatggggat caaaaccgtt ctcgatttgg cggattcaga
     7741 tatccggttt atccgtaaac attttaatgt cgtgctcgaa agaacggtgc gtgaactgcg
     7801 cggtgaaccc tgtttgcaac tggaagagtt tgcaccgacg aagcaggaaa ttatctgttc
     7861 ccgctcgttt ggtgaacgca tcacggatta tccgtcgatg cggcaggcca tttgtagtta
     7921 cgctgcccgg gcggcggaaa aacttcgcag cgagcatcaa tattgtcggt ttatctccac
     7981 gtttattaag acgtcaccat ttgcgttcaa tgaaccttat tacggcaata gcgcatcggt
     8041 aaaactgctg acgcccactc aggacagcag ggatatcatt aacgccgcta cgcgatctct
     8101 ggatgccatc tggcaagcgg gccatcgcta ccaaaaagcg ggcgtgatgc tgggggattt
     8161 cttcagtcag ggagtcgcgc agctcaattt attcgatgac aacgcaccgc gccccgggag
     8221 tgagcaattg atggcggtaa tggatacgct gaatgctaaa gagggcagag gaacactcta
     8281 ttttgccggg caggggatcc agcaacaatg gcagatgaag cgtgccatgc tttcaccatg
     8341 ttatacaacg cgaagttctg atttactgcg ggtcaaataa acatagcggc aggaaaaagc
     8401 gctcccgcag gagcgccgaa tggattagcg accgaacagg tcacgttttt tcgctttaaa
     8461 cggctgggaa atcaccacca gcaccgcgac aatcaggtaa gcgataaaaa taccgagcag
     8521 ccactgcggc atttccagac ctaaaaattc ccactgacgc tcggcgcaat cgccagaggc
     8581 gacaaacact tgcggcaccc atttatccag cggcagccat tccgggaaac gagccataaa
     8641 atcacaggtc gcaaacggcg aaggatagag ctgaagcatg gtgtgctcgt aagttaactg
     8701 cacaccgcgg aacgcactat acaaccagat aaccatcgct acataacgca gcggagtttt
     8761 cggggcgatc gcgccaatca gcgcagcacc cagaacgccg aataatgcgc agcgttcata
     8821 aatacagagc acgcaaggtt tcagtaacat cacatgctgg aaccacagtg ccgtcagttc
     8881 cagtgctaga gcggtaaacg ccatcaacag ccacgcgccc cggccttgtg aacattggtt
     8941 caaaaatcgc aacataatca tttccctgca atatgcatag agtgcgcagt ttaaaccaat
     9001 tcattcgctg cgccaccagg aggcagcaaa aataggacgc aattggacaa ttatcgggcg
     9061 aaaaggcaaa ccgggcattt atatgcccgg taagttgtta tcaaagcgtt gctatccagc
     9121 ccatttgcat aaaccattcg gttacagggg caagcgtaaa ctcgacgcag agcaagccga
     9181 cgagtgtcag gacgagggtg taaggcaggg ccatccacac catgcggcca taagagaggc
     9241 gaatcaatgg cgcgagtgca gaggtcagca ggaacaggaa cgcagcctga ccgttcggcg
     9301 tagcgacgga gggcagattg gtaccggtat taatggcgac cgccagcagc tcgtattgct
     9361 tcaacgtgat agcgccactt tccattgccg ctttcgcttc gttgatataa atcgtcccca
     9421 cgaagacgtt atccgaaatg gatgacagca gaccgttgaa aatatagaac agcgacagct
     9481 gagcatgttc cgatgcctgc aacacaaact gaataatcgg cgaaaacagt tgttggtcga
     9541 taatcaccgc gactaccgag aaaaacaccg tcaacagtgc ggtgaatggc agagactcgg
     9601 tgaaggcttt accgatagca tgttcatcgg tgacaccggt caatgatgtc gccagaataa
     9661 tgaccgacaa accaatcaag ccaacttccg ccagatgcaa cgccagcgca gtcaccagcc
     9721 agacgccaat aatcgcctgg acaatcagac ggattttatc ctgacgggta cgctggtggc
     9781 ggctttgatc gtcaaactgt tgcagtactt cgcggacttt ctccggcagc gtttcaccat
     9841 agccaaacca acgcagcttc tctaccagca ggcaggttaa caggccacaa atcagaaccg
     9901 gaacggtcac tggcgacatg cgcaggaaga aatcgccaaa atgccagcca gccgctttag
     9961 cgatgatcag gttctgtggt tcgcccacca tggtcatcac gccgcctaat gcggtgccga
    10021 caccggcatg catcatcagg ctacgcagaa agccacggaa ctgttccaga accactttgt
    10081 aatgcttgtc gatatgacta tcgtcttgca ggtcggtatc ttcggtacgg gaagaggcta
    10141 cgcggtgata aataccataa aaaccgactg caacgctgat caccaccgcc acgacggtta
    10201 aggcatcgag gaacgcggag aggaacgccg ccgccacgca aaaagagagc gacagcagca
    10261 ttttggagcg aatgcttaac agcaaacggg taaatatgaa cagcaatagc tgtttcataa
    10321 aatagatacc cgccaccata aacatcagta acagcaagac ttcaagattt gccgccacct
    10381 cttcacggac gtgttccgcg ctggtcatgc cgatgaatac cgcttcgata gccaacagac
    10441 caccggggag cagcgggtag catttcaggg ccatcgccag agtgaaaata aattccgcta
    10501 ccagcaacca gccagcgacg aaagggctga tgaggaaaat taacggattt acgattaaga
    10561 aaatgatgag ggcgagtttg taccagtcgg gggactggcc caaaaaattg cgccatagtg
    10621 cgcggcccca ggagatctcc atgatggttt cccttacctt agaaataatc aatgatgttt
    10681 ttatgtttaa acgcaaagct taacggtcag acaggagtga ggcaagtctt gatagtcaag
    10741 gggaaagaga tgcagaaaat gaagccttga tccctttttt cttctttttg tcttctatca
    10801 gcatagttag ccctctggta tgatgagtcc aactttgttt tgctgtgtta tggaaatctc
    10861 actatggtca ttaaggcgca aagcccggcg ggtttcgcgg aagagtacat tattgaaagt
    10921 atctggaata accgcttccc tcccgggact attttgcccg cagaacgtga actttcagaa
    10981 ttaattggcg taacgcgtac tacgttacgt gaagtgttac aacgtctggc acgagatggc
    11041 tggttgacca ttcaacacgg caagccgacg aaggtgaata atttctggga aacctctggt
    11101 ttaaatatcc ttgaaacact ggcgcgactg gaccacgaaa gtgtgccgca gcttattgat
    11161 aatttgctgt cggtgcgtac caatatttca actattttta ttcgcaccgc gtttcgtcaa
    11221 catcccgata aagcgcagga agtgctggca accgctaatg aagtggccga tcacgccgac
    11281 gcctttgccg agctggatta caacattttc cgcggcctgg cgtttgcttc cggcaacccg
    11341 atttacggtc tgatccttaa cgggatgaaa gggctgtata cgcgcattgg tcgtcactat
    11401 ttcgccaatc cggaagcgcg cagtctggcg ctgggcttct atcacaaact gtcggcgttg
    11461 tgcagtgaag gcgcacacga tcaggtatac gaaacagtgc gtcgctatgg gcatgagagt
    11521 ggcgagattt ggcaccggat gcagaaaaat ctgccgggtg atttagccat tcagggacga
    11581 taatcccttc cgtttaaaaa gcaaacccct caaacgaggg gtttttcgtt gtttttacag
    11641 atttcccatt ctcggcgggc aacgttccag caactcgacg ctgccgtctt cgttttgctg
    11701 ttccagcatc acatcaaatc cccacaggcg atgcacatgc ttcagcactt ctttgcgccc
    11761 ccgatccagc ggtgcgcgat tatgtggaat gtaacgcagc gtcagcgaac ggtcgccgcg
    11821 caaatccacg ttccagatct gaatattcgg ctccagatta cttaaattat attgagacga
    11881 taaccggtta cggatctccc gataaccttc ttcattatga atagcggaaa tctccagata
    11941 attatgccga tcgtcatcca gcacggtgaa gaagcggaaa tcacgcatca ctttcggtga
    12001 caggaactgg ctgataaaac tctcatcttt gaaatcacgc atcgcgaaat gcagcgtttc
    12061 cagccagtcg gaaccggcga tatccgggaa ccagtatttg tcttcttccg ttggcgactg
    12121 acaaatccgt ttaatatcct ggaacatggc gaacccgagg gcatatgggt tgatgccgct
    12181 gtaccacggg ctgttatagg gcggttggaa gaccacattg gtgtggctgt gcaaaaactc
    12241 cagcataaaa cgttccgtta ctttcccttc atcatacaga tggttaagga tggtgtagtg
    12301 ccagaaggtc gcccagcctt cgttcatcac ctgagtctgt ttttgcggat aaaaatactg
    12361 gctcacctta cgcacaatac gcaggatttc acgctgccat gattccagca gtggcgcatt
    12421 tttctccata aaatagagca ggttttcttg tggttcggac ggatagcggc gcgcttcagc
    12481 aaccgttttc tcttcctcgc gcttcggcag ggtacgccag agcatattga cctgactttg
    12541 cagatactct tcgcgactct tctgccgggc tttctcttct tgcagcgaga ttttttgcgg
    12601 gcgtttgtac cggtccacgc cgtagttcat cagcgcgtgg cacgagtcca gaagccgttc
    12661 tacttcatca acgccataac gttcttcgca ctcggtaata tatttacggg caaaaatcag
    12721 ataatcgaca atcgaactgg cgtcggtcca gctacggaat aagtaattgt ttttgaagaa
    12781 agagttatgc ccatagcagg catgagccat caccagcgct tgcatggtaa tggtgttctc
    12841 ttccatcagg taagcgatac acgggttaga gttaatgacg atttcatagg ccagtccttg
    12901 ctgaccgtgc ttatacagcc gttcagtctc gataaacttt ttaccgaatg accagtgcgg
    12961 atagttaatt ggcatgccga cgctggagta ggcatccatc atctgttctg aggttatcac
    13021 ttcaatctgg tgcgggtagg tatccagccg gtagagtttc gccacccggt ctatctctgc
    13081 cagataaaca tccagcaggt cgaacgtcca gtcgggtcca tcgctcaaac gtgtggtgtc
    13141 cttattcata gaatcgatcg tcgccatacg cgcacctcat tgttgtcggc gctctctgtg
    13201 tggagcacct catttcaagc atagaacacc tgttaaaaac cgcgtcgccg gagaattttt
    13261 ttctttgcga tttcttatta tcagagtgcc actaatccgc ttctgaacgg aattttatgc
    13321 tggataaaaa gggcgttcag caggagatac taaagacgcc atattgccgc agagtcaggg
    13381 agatgtgagc cagctcacca taaaaaagcc gtatgttgaa taatattttc aactgagtta
    13441 tcaagatgta attagattat tattctttta ctgtatctac cgttatcgga gtggctatgc
    13501 gagttgtcat actgggaagt ggtgtggtag gcgttgccag cgcctggtac ttaaatcagg
    13561 caggacatga ggtcaccgtc attgatcggg agccgggggc cgccctggaa accagtgccg
    13621 cgaatgccgg gcaaatctcc cccggatatg ctgcaccgtg ggcggcacca ggtgtgcctt
    13681 taaaagcgat taaatggatg ttccagcgcc atgcgccgct ggcggttcgt ctcgacggta
    13741 cgcagttcca gttgaaatgg atgtggcaaa tgttacgtaa ctgcgacacc agccactaca
    13801 tggaaaacaa agggcggatg gtacgtctgg cggaatacag ccgtgattgc ctgaaagcat
    13861 tgcgcgccga aaccaatatt cagtatgaag ggcgtcaggg tgggacgctg caactgttcc
    13921 gtaccgaaca acagtatgaa aacgcgaccc gcgatatcgc cgtgctggaa gatgccggcg
    13981 taccgtatca gctgctggaa tccagccgcc tggcggaagt ggagcccgcg ctggcagaag
    14041 tggcgcacaa actgacgggc ggcctgcaac tgcccaatga tgaaaccgga gactgccagc
    14101 tgtttaccca gaatctggcg cggatggcgg agcaggcggg ggttaaattc cgctttaata
    14161 cgcccgttga ccaactgctt tgcgacggcg agcaaatcta cggcgtgaag tgtggtgatg
    14221 aagtgattaa ggccgatgcg tatgtgatgg cgtttggttc ttactcgacg gcgatgctca
    14281 aaggcattgt tgatattccg gtgtatccgc tgaaaggcta ctcgctgacc attccaattg
    14341 cgcaggaaga tggtgcgccg gtatccacta ttcttgatga aacctacaaa atcgccatta
    14401 cccgtttcga taaccgcatt cgtgtcggcg gaatggcgga aattgttggt tttaacaccg
    14461 agctgttgca accgcgtcgt gaaacgctgg agatggtggt tcgcgatctc tatccacgcg
    14521 gcggtcatgt tgagcaggcg actttctgga ctggcctgcg cccgatgacg ccagacggca
    14581 cgccggttgt cgggcgtaca cgctttaaaa atctgtggct gaataccggt cacggcacgc
    14641 tcggctggac gatggcttgc ggttccggtc agttgttaag cgatctgctc tcggggcgca
    14701 cgccagcgat cccgtatgaa gatctaagcg tagcgcgcta cagccgtgga tttacgccat
    14761 cacgcccggg ccatttacat ggcgcacaca gctaaggaaa cgagatgacc cgtccgatac
    14821 aggccagcct cgatctgcag gcattaaaac agaatctgtc cattgtccgc caggctgcgc
    14881 cgtacgcgcg cgtctggtcg gtggtaaaag caaacgctta cgggcacggt attgagcgta
    14941 tctggagcgc gctcggggcc accgatggct ttgcattgct taatctggaa gaggcaataa
    15001 cgttacgtga gcgcggctgg aaaggaccga tcctgatgct ggaaggattt ttccatgctc
    15061 aggatctgga gatttatgac cagcaccgcc tgaccacctg cgtacacagc aactggcagc
    15121 tcaaagcact gcaaaatgcg cggctaaaag caccgttgga tatttatctt aaagtgaaca
    15181 gtgggatgaa tcggttgggc ttccagcccg atcgcgtgct taccgtctgg cagcagttgc
    15241 gggcgatggc gaatgttggc gaaatgaccc tgatgtcgca ttttgccgag gcggaacatc
    15301 ctgatggaat ttccagcgcg atggcgcgta ttgagcaggc ggcggaaggg ctggagtgtc
    15361 ggcgttcgtt gtccaattcg gcggcgactc tgtggcaccc ggaagcgcat tttgactggg
    15421 ttcggcctgg cattattttg tatggcgctt cgccgtccgg tcagtggcgt gatatcgcca
    15481 ataccggatt acgtccggtg atgacgctaa gcagtgagat tattggtgtc cagacgctaa
    15541 aagcgggcga gcgtgtgggc tacggcggtc gctatactgc gcgcgatgaa cagcgaatcg
    15601 gcattgtcgc cgcaggatac gccgacggtt atccgcgcca tgcgcctacc ggtactcctg
    15661 ttttagtgga cggcgtgcgc accatgacgg tggggaccgt ctcgatggat atgctggcgg
    15721 ttgatttaac gccttgcccg caggcgggga ttggtacgcc ggttgagctg tggggcaagg
    15781 agatcaaaat tgatgatgtc gccgccgctg ccggaacggt gggctatgag ttgatgtgcg
    15841 cgctggcgtt acgtgtcccg gttgtgacag tgtaacttgt tatcgctgga tgcgaccagc
    15901 gtcgtatcca gcgttacaaa acacattaaa cttaagattc ggcttcttcc tccgctaccc
    15961 gaacaccaat cttcaacact tcattatcct ctttctcggc caccgtccag atcattccgg
    16021 caaattctac ctgatcaccg acaaccggtg ccgcgcctaa caactgctgg acaatttcac
    16081 ccagcgtttg ctgcttatca cgatattctc gcccgtcttc caggccatat atcagcgcca
    16141 catcagcata tttggcactg gcttcgagaa tgaagtcacc aaagaagcgt tgatctagcg
    16201 cgaccggtgg cgactggctg aacagtttac cgagcgccgg gagatcgcgt tcccgaccaa
    16261 ttacacacaa cacatcgcct tcgcgcagtc gggtgctgcc ggtaggatga agcaactggt
    16321 tatcacgaaa cagtgccgca atacgcgtct cttttggcat atgcagatcg cgcagtgccg
    16381 cgcccacgca ccatttatcg gcactcaact gataaacaaa ctgctcccac ggattttccg
    16441 gatgaatatc cagaccaacg cgtgacaccg gacgtcccac tggcggaacg accactttgg
    16501 cttttttcgc cgcccacgag agtgatgtcc cctgcaagag tagtgaaacc agaaccacaa
    16561 agaaggcgac attaaagaac agtcgcgcat tctccagccc cgccatcatc gggaacacgg
    16621 ccaggatgat tggcaccgcg ccgcgtaatc ccacccagct gataaacacg cgctcacgca
    16681 gattgaagcc acggaacggt agcaatccgg caaataccga aagaggacgg gcgaagaaaa
    16741 tcatccatgc ggacaaaatg agcgccggaa tggcaatagg cagcagatcg cttgggttaa
    16801 ccaacagccc cagcaccagg aacatggcga tttgcgccag ccaggcgagg ccgtcgaaat
    16861 tttgcagaat gccgtagcgg ttgcgaatcg gccgattacc cagcagaaaa ccgcacagat
    16921 aaacagccag aataccgctg ccttccagcg ccgtggttaa agcaaaaatt aaaataccgc
    16981 cgcttaatgc cagcaatgga tataaaccgg cgggcagggc gatgcgatta atcatttgca
    17041 gcagtaaata accaccgcca agaccgatga caatcccgag gccaaattgc tgcaaaatat
    17101 cgaccacgaa catccagctg acactgcttt catgctgctg gatcatcgca atcagggtaa
    17161 tcgtcagaaa gaccgccatt ggatcattac tgccggattc aatttccagc gtcgagccaa
    17221 cacgttcgtt aagcccctta ccacccagca gagaaaagac cgctgcagca tcggtggagc
    17281 cgacgatagc gccgattaat aatccttcaa tcaaatcaag attaaacagc cacgccgcca
    17341 tcatgccggt taaaccagag gtgataagca cgcccagcgt cgccagcgac agtgccggtc
    17401 ctaacgccac acgaaaggaa ctggcctgag tgcgcatccc gccgtcgagc aaaataatcg
    17461 ccagcgccag attacttacc atgtaggcga agggataatt atcaaacggg ataccgccga
    17521 cgccatcaac ccccgccagc atgccgatcg ccagaaaaat aaccagaatg ggaatgccaa
    17581 gacgggaaga aaatgaacta agtaaaatac tgctggtgac aaggatggat cctaagatga
    17641 aaaggctaat tattgttgtg gcatccaacg gtcggttact cctgattacg ctgtctctta
    17701 tataaaccct accatattag cggcagagac agcgttttac ttagccctga agcgtgattt
    17761 ttttacattt taagaacagg atgaccgcta atcgtcaact gggtgccttc ctgggtgtta
    17821 ttcagaatgg catgtgcgcc caacggcagt gtaaccgtgc gttgttcatg accaaaatcg
    17881 aggccggtaa tgagtggaat cgacaggcgg gaacgcaaaa acgcgtacac tgactccagg
    17941 ttgtaacctg cgtcataatc attaggcgtg ctgccgctaa agctaccgag aataatcgcc
    18001 ttctgacgtg gcaaaattcc agcatgatag agctgcaaca gcatacgttc gacgcggaaa
    18061 gggtgctcgt taatatcttc cagcaccaga ataccgttct caatttttgg catccacggt
    18121 gtaccaatca gtgaaatcag catcgcgaga ttgcctcccc acagcgtgcc ttcggtctga
    18181 catgtcgggc cttccccttg ccattcaatg gtgaaggttt cattgcgtaa cgccagccag
    18241 aaatggtgct cggtaaaggc gttcagttca tccgcgccaa agtttgctac cagcatgggg
    18301 ccgctaaagg tgatgacatt gccatgcgcc agaagaccgc actgaatggc ggtaaaatcg
    18361 ctatgtccgc aaatgagcaa cgggtcatgt tgttggcgag ccaccagtgc ctgccagtca
    18421 atatctgcca gtaaacgact ggcaccgtaa ccgccgcgta cagccagcac gatggtgttg
    18481 ggggtagtca gtctggcaag agaattgaga tcttccagac gctccgtttc cgtacccgca
    18541 aaacgctcac aacgacgggc aataacctcg acgttattga cctgatgccc cgcgtcggta
    18601 aggcgctgga taccacgcag cgcggcgtgc tgtttaatgc agtaacccga tggggcaatt
    18661 aagtgaaaca gagacatggc aattccttgc tgacaacaga aacgaaatgt atatcatgcc
    18721 gcttaggtgt gccgttgtca cctcaacggc gattccaggc tataaggata gaagaagtga
    18781 aattgagatg gtttgccttt ttgattgtgt tattagcggg ttgttcatcg aagcatgact
    18841 atacgaaccc gccgtggaac gcgaaagttc cggtgcaacg tgcgatgcag tggatgccaa
    18901 taagccagaa agccggtgca gcctggggcg tcgatccaca attgatcacg gcgattatcg
    18961 ctatcgaatc gggtggtaat ccgaacgcgg tgagtaaatc gaatgccatt ggtttgatgc
    19021 agttaaaagc ttcaacctcc ggacgtgatg tttatcgccg tatgggctgg agtggtgagc
    19081 cgacgaccag cgagctgaaa aatccggagc gtaatatttc aatgggggcg gcttacctga
    19141 atattctgga aaccggcccg ctggcaggca ttgaagatcc gaaggtactg caatatgcgc
    19201 tggtggtgtc atatgctaac ggggcaggtg cgctgctacg gactttctcg tcagatcgga
    19261 aaaaggcgat cagcaaaatc aacgatctgg atgctgacga gttcctcgat cacgtagcgc
    19321 gaaatcatcc tgcgccgcag gctccgcgct atatctacaa acttgagcag gcactggacg
    19381 cgatgtaaat cagtcgcgca ctttgtccgc tttctcccgg gcttctcgct cgagagagaa
    19441 aataatccgc tgtaattgcc gctccaccgt cgggctgacg ttaagaaaac ggaagctcag
    19501 acggggagtg gtgatggttt cattcttgcc atcaatcact ttgcgctcgc tgatggagat
    19561 taactgggcg tcaaagtgaa aaacacccca ttgccccatg ttgacttcaa tctgagcgaa
    19621 gcgcatgcct tcgtgcaatc cggcaggctt tgctgtttcc agtaatgcgc ccatgccgcc
    19681 taacgacaaa tcatacaggc ggaaacgtaa cgtactgtta tccgccagtt tggtctggca
    19741 aaaatatggc ggatggagtg gggcggagat gcggaaatat cggcgtcgtt gtacaaacca
    19801 caaggtggga ggcggtacgg taataaatgc cggaagctgc aagtattcac tctgctgtag
    19861 ttgttcaaca gtaaactcga ctttcgcacc ctgggtttcg gcggtaatgg taatgtgctg
    19921 cgcctttaac acagcgttgt tatcttcggc ttgactgccg aaatccagca ccagtttatc
    19981 cggggttatt gccagtattt tgctgatcag ctgcccgcca ttccaactga tacgcaaagg
    20041 aattgcggct ttgtgcaaat cgcgtaacac gcccaggacg gctaacggat tttgtttcag
    20101 gaactgctca tggtaatgac tcacgcggaa aaactcctga ctgacaaact gtcttaggtt
    20161 tatcggtcac agttaacaaa acttaataca aatgcgtgaa tattttttac atgtcgttct
    20221 taaatcagcc gacatacgcc cagcatcgat cccgcccttg cctatactta gagcgttgac
    20281 gtaagcattt cttgcgtccg attcatcgaa acgagggcct gaacatggga attattgcct
    20341 ggattatttt tggcctgata gccggcatta tcgccaagct aatcatgccg gggcgtgatg
    20401 gtggtggatt tttcctgacc tgtattcttg ggatagtcgg tgcggtggtc ggcggctggc
    20461 tggcgaccat gtttggcatt ggcggctcca tcagcggttt taatttgcac agcttcctgg
    20521 tggcggtagt gggcgctatc ctggtgctgg gcgtattccg cctcctgcga agagaataag
    20581 attttcataa ggcggatagc gatacagatg ccgctatccg ctttcacatc agaacgtata
    20641 ctcgatacct gcccagaaat tgcgaccttc ttcaataaac ccttcactgt aggcatattt
    20701 ggtatcaaac aggttattga ccgacgcatt gacgctgaag ccatgaccta aggtgtaatc
    20761 ggctcgaatg tgggtcaccg caaaaccggc ggctttttgt gaaccgtcac tgttgctgta
    20821 gctggaggaa cgcgcctctt ccgacagcgt tacgcttaac ggctcccacg gtttgagagt
    20881 catccatgcg gtcattgtct gcgttggcag atcggttatc ttgccgatgt ctttgcgttt
    20941 ggcatcagcg tggatcaggg cgtagctcaa tcctacatcc agtatattgc tgattttccc
    21001 cttaataccg gcatccagac cgctgtaatc caccgtgccg ctgttctgat tttgaatggt
    21061 atcggcatcg atattgtgcg agaggatggc atcactcacc cggttatagt aaacgctgac
    21121 ctcaaacccc cagtcgtgcg tgaaggcacc attccaggtt aaatccaccc cgcgcgcgcg
    21181 ttccggtttg agctgcgggt taacaatcgc tatctggtta tacgcaggtt tggacgtggt
    21241 atagcgttct ttcagcgtcg gaaagcgtgt gcggtcatag tacgaaagcg ccagcgtgtc
    21301 ttcattggca aagtggtatt tccccatcac ctgccagtta aaagctgact gattgttgtc
    21361 gtcataatgg gtgatgcttc catctttctc atgttttttc gcttctacgc tatcgcgcca
    21421 gtcatagctg attccagcca cgacatcgac attatcggca gcagcccatt gatattcact
    21481 ggcgagcgac caggtacgat cttcatagcg atcgtaagcg gcgtgcggcg cacctttttc
    21541 ccggtgtacg tcatctttcc agttaacggc aaacgacagc agatcgtttt cacgcacatc
    21601 ggctgccagt tgtaacccgg caccgtcgct gtaatcggaa taatggctgt agctgccttt
    21661 tttatttttc aaatcagcca gcgagttgta catcatcagc gtattttcaa aggtatcgcg
    21721 atacagccga cttttcaggg taaaacgatc gtttagttgg gtcgttccct gataataaaa
    21781 actttctttg tcatactctg gccactgcca gtagcgtgat ttttgaccac tatttccact
    21841 gtatggcgga ttatcttttt caccatcctg cttaatgtaa gtcaatgtgt attcatcgtt
    21901 ttcgcgtggt gtaaaaccga gcttcacaat gccgcgttta tcatcagccg atgaattaat
    21961 catcttgccg tgtttgcctg caatatcatt atttacacca tgcggcaggc cgagaaaatc
    22021 ctgctttagc tggctaccgc tgacttgcaa ataccccaga tcgttgctgg cggcaaatga
    22081 agcatgcata tcgtaggcat tgtcctggct acggctccat ccctggcgat atcccagatt
    22141 tgcttccaga ggttttgttg gcttctgggt ggtgatatta atggctccgc ccatctgatt
    22201 aggcccctga agcagcgacg aatacccttt ggaaacttca actgccccca aattgttggt
    22261 cagaatccgc gccagatcga ggttgccgtc atagggaaca taaatgggca caccgtcgaa
    22321 atagactggc acctgacgac tatcaaagcc acgaactttg acctgttctt cgttgcggct
    22381 acctgacttt tgcagcacca cgccggggac gacacttaat gcctgagcga cattctgttt
    22441 atccagggcc ttcatggtgg ggtgatcgag aacggtggtc gtcgtcgatg ataccggact
    22501 ggaccatacg gtcagcgtgt cactctcctg agccgctaag gcctgctggg taaaggcaag
    22561 tgataatgct gtgcagagat aacgtttttt gagtctcata gaataaattc cgtttaacaa
    22621 agaattaaat agaggctgcg ggttgcagac tgactttaat gtcagctggt gtcgcgtaat
    22681 aaggcgcgga ggtaataaaa aggcgaatgc cgcagtcgag gtaatttttg agtgttgtca
    22741 gattaattcc gccggtcaga gccagcgtgc agtgaggagc cagcgacggt gctatttgcg
    22801 caatttcagt tgcctgttgc ggattaaatt tgtcgagctg aagtacgtct ggttgcgcgc
    22861 gtaacgcggc gattgcctct ttcggcgtgt cggcttcaac aactattttc ttctcgggtg
    22921 cgtggcggcg taactgattg attgcgcctg accagtcctg attgtcatga agaaaatgac
    22981 ggtggttggc aaacaataat atggtttccg cacatccggc acgatgaatc agtcctccgg
    23041 cagccagaat tgcctgcgag gccagtaagc gggtgcccgg aattgctttt cgggtgcagg
    23101 cgatattgcc atcagggtaa cgttcacgaa gtaacgccag catttgagcg agataatcag
    23161 aaacaccgca actccactcc agcacattct ggactgcctt ccatccctga tgaagtgctg
    23221 cggcattacc ctgcgcacgg attagccgct gacccgcgtt cgcttgtgaa ccgtcgctga
    23281 ctgcgtcatc aatagttaat cccagcgtag ttaacatctt acacgcgaca gaaattccgc
    23341 tgacgcagcc gccctgacgg tgaaaaaact ctatatagcc atgctggtgt ccaatattta
    23401 atgctcgcgt ggtcaggtca ccgccctgga tatcttccag aagtaacgca tcgatttgtg
    23461 cctgagaaag gaaaatcatc gcagtgcctc ttgcggtacg ctatcccacc agatgagcgc
    23521 ccagtcgcgg ctggcagggg cgagggcacg agcatcttgt tgttgatacc agcgatataa
    23581 ccgctcacgc tcatcgtcgt taagcgcgcc cagactccag ctgacgttct gctcaaaacg
    23641 ttcccaggtg ctgttatcct gttggcaatt ttgtccgcgg ataaaatcaa cgtgagcata
    23701 aattcccatc tgatacagga cgttgagcgc aaagatatag ttgggtaatt cgaccacttc
    23761 ccgaccagcc gcccgctgaa tggctggaga gacgaacgag gtgctgacca gatgagtcgt
    23821 atagacgcga agccgtgcct gattattcag tttgctcatt gcctggcgca tatcagccac
    23881 cagagtggag cgggaagcca cggcaatatc gcagcgtggc aggtcactcc agtcctcttc
    23941 ccaggcgcgc tggatccagt ggacattgtc tgcctttagc gcggcggcgc gacgggcggc
    24001 aacgttcagc atcccctgac tgtaatcaac gccataaatc gtcgtgagtt tgtccgccag
    24061 cgccagcgat acggtacccg gtccgcagcc catatcgaat aaggtttgcg cgccttgcag
    24121 gtcaattttt gaggtcagtt gttgcaggta gctgtccgtc ggcgaggcgc aattttccgc
    24181 cattttttcc gcccgttgat cccagtgatc cggcgttttt tctgtgcgat gggccagcct
    24241 gagttgctga aggtaaaggt ctgcaaaatc aatatcgtca atgagcatga tttttcctta
    24301 gtgcttaatt gcagagaggt gatggtgaat ctggtcggca gaaacccggt acagggcagc
    24361 cagttttttc gttgttaact gttcggtcgg taagccttgt gttactcgtc catctggctc
    24421 tacctggata atgctgtcgg caatggcgtt tgcatggagc ggatgatgag tcgacattaa
    24481 cattgtcatg ccgttatttt tgagctgcgc cagcgtgtcc agaagttgga tctgatgacc
    24541 gaaatcgagg ctagaggcag gctcatccag taataacaag cgcggttgct ggacgagtgc
    24601 acgggcgatt aaagccagct gacgttcacc gccgctaagc gtattccagc gtcgtgtcgc
    24661 cagatgcaaa atgcccagct tttccagttg ctccgttgct ttcaggcgtt cttgcttacc
    24721 gggaacggaa aaagcgccta tggtgggggc ctggcccatc aacaccatat ccagcacact
    24781 aaaagcaaaa atgccgtcgt gcgcctgcgg cacccaggca atagcctgcg cgcgctggcg
    24841 atggctgagt gtctgcacag ggaggctatc cagtaagatc tcgcctttta ataacggtat
    24901 aacgccaaga atggagcgca tgagtgtggt tttgccgcaa ccattggctc ctaacagaca
    24961 acaaattgtt ccttgcgaca gggtgaagga cacgtcgcgt agcaccggat ggcgggagta
    25021 acccagagac gcatgacgga cggcaagcaa agtcactggc gacctccgcg taacaacaaa
    25081 aacaggaaaa acggtgcgcc aacaaaagcg gtcagaatgc caagtgggat ttctgttgtt
    25141 ccaatgctgc gtgccagcgt atcggtaagc aggagcagaa tcgcgccagt acacattgcc
    25201 atgggcaaca gctgctgatg gttatgaccg gtcaacaagc gggcaatgtg cggaacgacc
    25261 aggccgaccc aaccaattat gcccgctatt gctaccgtgc tggcggtgat gagtgtggca
    25321 cagacaatca atccaaagcg cagacgtgtc acgttcagtc ccagcgaacg ggcttcgtcg
    25381 tcggaaagcg tgagtaaatt catgcgccag cggagaaaaa acaggggcaa ggagcctatg
    25441 agaatgatcg gtgcagcata gcagagatcg cgtaaggtca cggtggacag cccccccagc
    25501 aaccagaagg taattgacgg taactgggta taaggatcgg ccagcgtttt tattagcgat
    25561 atccccgctc cgcacaacgt tcctaacgca atgcctacca gcaccagggt taagatcgga
    25621 tcgtggcggg tcacccgacg cgtaatcaac cagacgcccg cgaccaccat cagcccgccg
    25681 caaaatgcgt acagctggat gtaaacaatg gataatcccc acaggattgc cgcgcaggca
    25741 ccaagtccgg caccggcgga gacgcctaaa atatccggtg acactaaggg attacggaac
    25801 attccctgat aggtggttcc ggcccctgcc agcgccgcgc ccagaaacag cgcagcaaga
    25861 atacgcggca cgcggatttg ccagaagaca atttgttcct gcacagtaac attttcttga
    25921 cgaagaatga gcgccagcag ttgctgcata tcgagatgat aagccccact tacggcggca
    25981 aaagtgatac tgatagctgc cagcagcaaa atacatccgt ttacggttct cattagctcg
    26041 ccaccaattt ttgatagtcg gcgtcactta atgggcaatg ccagaacagt tgggcgtaat
    26101 gctgcatgtc acttttaaac tggcgattga tggcgggatc cagccaggcg tgaagtctgc
    26161 gtaatcccag aagacggttg attcccggcg gggcatccag ccagccaaag ggcaggccac
    26221 ttaaaaataa gatgcgttgt tccgcaaccg ctttcacgcc ctgccagaga ggatcacgac
    26281 gaataaaatc tgcagtaacg gcctcctgaa ccagaataat atccggctgc cagcgcagga
    26341 gattttccat ggaaacctgt gtcagaccgt ggcgatcggc tatttgcgcg acgttgtgca
    26401 aacctaataa ttccgccgcc tcggtatgca acgaaccctg taagcccgtt tccagccctc
    26461 gaggaccgcg cgcagcataa aagctgagat tagcggcggg tgaggtggcg aatgcctgag
    26521 cttcaccaac gaagcggctg gcgagattgg cttgttctgc ggcgcggtgc tcttcgccta
    26581 acgttttgcc aagcgttgtt aactgttctg ctgattgttc cagtttcccg ttaagcaata
    26641 accagggtat ttgtgtctgt tcgctaacct gccgtgcctg ggagatccag gtttcatccg
    26701 tattgccgca atcaacgacc aaatcgggat gtaacgccat aagcccttcc agcgagagtg
    26761 tactggcgcg cccggcgagt cgtcccagcc tggggaactg gcgaatgtgc tctggcaatg
    26821 gaattaatgc ctgacgggca aaatcaaaag aggaaaaacc gaccattttt tctggcgcga
    26881 cagccagcaa tagtaaatcg gcgggcgcgc ctgcgctgac gatgcgtgta atgttttggg
    26941 cttcgggaag cgtcagtgag gcccagaatt tgactgattg tgcaaaggac ggcagatgat
    27001 gcaggaacaa tgtcgacgca agaatctgtg taaacctccg tctggttagt gacataagta
    27061 atatcctga
//
LOCUS       DAZUCC010000049        26498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZUCC010000049 DAZUCC010000000
VERSION     DAZUCC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.049"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            15..1484
                     /gene="gspA"
                     /locus_tag="ACHY09_001783"
     CDS             15..1484
                     /gene="gspA"
                     /locus_tag="ACHY09_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFS3884763.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSSQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLMEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTSVFT
                     AKHWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTDPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHQWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1486..1905
                     /gene="gspB"
                     /locus_tag="ACHY09_001784"
     CDS             1486..1905
                     /gene="gspB"
                     /locus_tag="ACHY09_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFS3884764.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2143..2454
                     /gene="rpsJ"
                     /locus_tag="ACHY09_001785"
     CDS             2143..2454
                     /gene="rpsJ"
                     /locus_tag="ACHY09_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS3884765.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2487..3116
                     /gene="rplC"
                     /locus_tag="ACHY09_001786"
     CDS             2487..3116
                     /gene="rplC"
                     /locus_tag="ACHY09_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS3884766.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3127..3732
                     /gene="rplD"
                     /locus_tag="ACHY09_001787"
     CDS             3127..3732
                     /gene="rplD"
                     /locus_tag="ACHY09_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS3884767.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3729..4031
                     /gene="rplW"
                     /locus_tag="ACHY09_001788"
     CDS             3729..4031
                     /gene="rplW"
                     /locus_tag="ACHY09_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS3884768.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4049..4870
                     /gene="rplB"
                     /locus_tag="ACHY09_001789"
     CDS             4049..4870
                     /gene="rplB"
                     /locus_tag="ACHY09_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS3884769.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4887..5165
                     /gene="rpsS"
                     /locus_tag="ACHY09_001790"
     CDS             4887..5165
                     /gene="rpsS"
                     /locus_tag="ACHY09_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS3884770.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5180..5512
                     /gene="rplV"
                     /locus_tag="ACHY09_001791"
     CDS             5180..5512
                     /gene="rplV"
                     /locus_tag="ACHY09_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS3884771.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5530..6231
                     /gene="rpsC"
                     /locus_tag="ACHY09_001792"
     CDS             5530..6231
                     /gene="rpsC"
                     /locus_tag="ACHY09_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS3884772.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6244..6654
                     /gene="rplP"
                     /locus_tag="ACHY09_001793"
     CDS             6244..6654
                     /gene="rplP"
                     /locus_tag="ACHY09_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS3884773.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6654..6845
                     /gene="rpmC"
                     /locus_tag="ACHY09_001794"
     CDS             6654..6845
                     /gene="rpmC"
                     /locus_tag="ACHY09_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS3884774.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6845..7099
                     /gene="rpsQ"
                     /locus_tag="ACHY09_001795"
     CDS             6845..7099
                     /gene="rpsQ"
                     /locus_tag="ACHY09_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS3884775.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7264..7635
                     /gene="rplN"
                     /locus_tag="ACHY09_001796"
     CDS             7264..7635
                     /gene="rplN"
                     /locus_tag="ACHY09_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS3884776.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7646..7960
                     /gene="rplX"
                     /locus_tag="ACHY09_001797"
     CDS             7646..7960
                     /gene="rplX"
                     /locus_tag="ACHY09_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS3884777.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7975..8514
                     /gene="rplE"
                     /locus_tag="ACHY09_001798"
     CDS             7975..8514
                     /gene="rplE"
                     /locus_tag="ACHY09_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS3884778.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8529..8834
                     /gene="rpsN"
                     /locus_tag="ACHY09_001799"
     CDS             8529..8834
                     /gene="rpsN"
                     /locus_tag="ACHY09_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS3884779.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8868..9260
                     /gene="rpsH"
                     /locus_tag="ACHY09_001800"
     CDS             8868..9260
                     /gene="rpsH"
                     /locus_tag="ACHY09_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS3884780.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9273..9806
                     /gene="rplF"
                     /locus_tag="ACHY09_001801"
     CDS             9273..9806
                     /gene="rplF"
                     /locus_tag="ACHY09_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS3884781.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9816..10169
                     /gene="rplR"
                     /locus_tag="ACHY09_001802"
     CDS             9816..10169
                     /gene="rplR"
                     /locus_tag="ACHY09_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS3884782.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10184..10687
                     /gene="rpsE"
                     /locus_tag="ACHY09_001803"
     CDS             10184..10687
                     /gene="rpsE"
                     /locus_tag="ACHY09_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS3884783.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10691..10870
                     /gene="rpmD"
                     /locus_tag="ACHY09_001804"
     CDS             10691..10870
                     /gene="rpmD"
                     /locus_tag="ACHY09_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS3884784.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10874..11308
                     /gene="rplO"
                     /locus_tag="ACHY09_001805"
     CDS             10874..11308
                     /gene="rplO"
                     /locus_tag="ACHY09_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS3884785.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11316..12647
                     /gene="secY"
                     /locus_tag="ACHY09_001806"
     CDS             11316..12647
                     /gene="secY"
                     /locus_tag="ACHY09_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS3884786.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12679..12795
                     /gene="rpmJ"
                     /locus_tag="ACHY09_001807"
     CDS             12679..12795
                     /gene="rpmJ"
                     /locus_tag="ACHY09_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS3884787.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12942..13298
                     /gene="rpsM"
                     /locus_tag="ACHY09_001808"
     CDS             12942..13298
                     /gene="rpsM"
                     /locus_tag="ACHY09_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS3884788.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13315..13704
                     /gene="rpsK"
                     /locus_tag="ACHY09_001809"
     CDS             13315..13704
                     /gene="rpsK"
                     /locus_tag="ACHY09_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS3884789.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13738..14358
                     /gene="rpsD"
                     /locus_tag="ACHY09_001810"
     CDS             13738..14358
                     /gene="rpsD"
                     /locus_tag="ACHY09_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS3884790.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14384..15373
                     /gene="rpoA"
                     /locus_tag="ACHY09_001811"
     CDS             14384..15373
                     /gene="rpoA"
                     /locus_tag="ACHY09_001811"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS3884791.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15414..15797
                     /gene="rplQ"
                     /locus_tag="ACHY09_001812"
     CDS             15414..15797
                     /gene="rplQ"
                     /locus_tag="ACHY09_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS3884792.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15904..16272
                     /gene="yhdN"
                     /locus_tag="ACHY09_001813"
     CDS             15904..16272
                     /gene="yhdN"
                     /locus_tag="ACHY09_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFS3884793.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILADDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16283..16708
                     /gene="zntR"
                     /locus_tag="ACHY09_001814"
     CDS             16283..16708
                     /gene="zntR"
                     /locus_tag="ACHY09_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFS3884794.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16764..16982
                     /gene="arfA"
                     /locus_tag="ACHY09_001815"
     CDS             16764..16982
                     /gene="arfA"
                     /locus_tag="ACHY09_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFS3884795.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16979..17389)
                     /gene="mscL"
                     /locus_tag="ACHY09_001816"
     CDS             complement(16979..17389)
                     /gene="mscL"
                     /locus_tag="ACHY09_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS3884796.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPTPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17519..18895)
                     /gene="trkA"
                     /locus_tag="ACHY09_001817"
     CDS             complement(17519..18895)
                     /gene="trkA"
                     /locus_tag="ACHY09_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFS3884797.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18917..20206)
                     /gene="rsmB"
                     /locus_tag="ACHY09_001818"
     CDS             complement(18917..20206)
                     /gene="rsmB"
                     /locus_tag="ACHY09_001818"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS3884798.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLNWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPPADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20252..21199)
                     /gene="fmt"
                     /locus_tag="ACHY09_001819"
     CDS             complement(20252..21199)
                     /gene="fmt"
                     /locus_tag="ACHY09_001819"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS3884799.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21214..21723)
                     /gene="def"
                     /locus_tag="ACHY09_001820"
     CDS             complement(21214..21723)
                     /gene="def"
                     /locus_tag="ACHY09_001820"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS3884800.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21853..22977
                     /gene="dprA"
                     /locus_tag="ACHY09_001821"
     CDS             21853..22977
                     /gene="dprA"
                     /locus_tag="ACHY09_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFS3884801.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22949..23422
                     /gene="smg"
                     /locus_tag="ACHY09_001822"
     CDS             22949..23422
                     /gene="smg"
                     /locus_tag="ACHY09_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFS3884802.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23451..23993
                     /gene="yrdD"
                     /locus_tag="ACHY09_001823"
     CDS             23451..23993
                     /gene="yrdD"
                     /locus_tag="ACHY09_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFS3884803.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23998..24570
                     /gene="tsaC"
                     /locus_tag="ACHY09_001824"
     CDS             23998..24570
                     /gene="tsaC"
                     /locus_tag="ACHY09_001824"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFS3884804.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24575..25393
                     /gene="aroE"
                     /locus_tag="ACHY09_001825"
     CDS             24575..25393
                     /gene="aroE"
                     /locus_tag="ACHY09_001825"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS3884805.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25390..25647
                     /gene="yrdB"
                     /locus_tag="ACHY09_001826"
     CDS             25390..25647
                     /gene="yrdB"
                     /locus_tag="ACHY09_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFS3884806.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25623..26177)
                     /gene="yrdA"
                     /locus_tag="ACHY09_001827"
     CDS             complement(25623..26177)
                     /gene="yrdA"
                     /locus_tag="ACHY09_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS3884807.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSAKNYVKWKDEYLDQGNQTQP"
     gene            complement(26221..>26498)
                     /locus_tag="ACHY09_001828"
     CDS             complement(26221..>26498)
                     /locus_tag="ACHY09_001828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3884808.1"
                     /translation="RLSAHCREVAYITLSSFRVNPEFQDFFSLLLRPSCEVFHVVVST
                     EAHYRDPNFLHKYFFDLFFCLLLFHPFCCIRTQNGAFLHAKRLAQGQ"
BASE COUNT         6743 a         6269 c         6933 g         6553 t
ORIGIN      
        1 aaacaggaag ttctatgtct acgagaagag aagttattct ttcctggttg cgtgagaaac
       61 gtcagacctg gcgtctgtgc tatttgttgg gtgaggctgg aagtggaaaa acctggctgg
      121 cgcagcaact acaaaaagat aaacatcgcc gtgtgattac tttaagcctc gttgtttcct
      181 cgcaaggtaa ggccgcatgg atcgttaccg acgataatgc ggctgaacag ggctgccgtg
      241 acagcgcctg gacgcgagat gagatggcgg gtcaattact gcatgcgctt catcgaactg
      301 atagtcgctg ccctcttatt attattgaaa atgctcacct gaatcaccgc cgcatacttg
      361 atgatctcca acgcgcgata tctcttattc ctgacggtca atttttgctg atcggcagac
      421 ccgatcgaaa agtcgaacgt gattttaaaa aacagggcat tgaacttgtc tcaacaggac
      481 gtctgatgga gcacgagctt aaggcaagca tccttgaagg tcagaatatt gaccagcccg
      541 atctcctttt aactgccaga gttctgaaac ggatagcttt attatgtcgg ggagatcgca
      601 gaaagctggc gcttgccggc gaaacaatta gcttattaca gcaagccgag cagaccagcg
      661 ttttcactgc aaaacattgg cgaatgattt accgcgtatt aggtgataac cgcccccgga
      721 aaatgcagct tgccgtggtg atgagtggaa ccattatcgc acttacctgt ggttggctac
      781 tgctgtcctc tttcacagat ccgttccccg ttcctgcgtg gctggtaccc gtaacgccag
      841 tagttaaaca agatatgact aaggatattg ctcatgtagt gatgcgcgat agtgaggcgc
      901 taagcgtgtt atacggcgta tgggggtacg aggtcccagc tgattcggcc tggtgcgatc
      961 aggcagtaag agcggggctg gcatgtaaaa gtgggaatgc ttctttacaa acattggtag
     1021 atcagaattt gccctggatc gcatcgctta aagtcgggga caaaaaattg cctgttgtgg
     1081 tcgttcgcgt cggtgatgct accgttgatg tcctcgtagg gcagcaaacc tggacgctga
     1141 cacatcaatg gtttgagtcg gtatggacgg gtgattatct tctgttatgg aaaatgtcgc
     1201 cggaagggga gagtaccatt acgcgtgata gcagcgagga agagattctc tggctggaaa
     1261 cgatgttaaa ccgcgcattg catatttcga cagaaccttc ggcagagtgg cgtcccttgt
     1321 tggtagaaaa aataaagcaa tttcaaaaaa gccaccactt gaaaactgac ggtgttgttg
     1381 gcttttcaac gttagtacat ctctggcagg tggcagggga gagtgcctac ttatatcggg
     1441 atgaagcaaa tatttccccg gaaacaacag tgaaggggaa atgacatgtt tgaattctat
     1501 atcgccgcgc gtgagcagaa agaaacaggt caccctggta tcttctcccg acaaaaacac
     1561 agcaccataa tatatgtcat ttgtttattg ctgatttgcc tgtggtttgc aggaatggtg
     1621 ttggttggtg ggtatgccag gcagctatgg gcgctttgga tagtaaaagc agaagtcact
     1681 gtagaggctg aaacgcccgc ttttaaacaa tcaacacaac actatttctt caaaaaacag
     1741 ccactccccg tcgtcgaatc cgtcgaggag gaagatgacc cagacgtcgc ggttgagaat
     1801 gctccctctt cttctgaaga cgaagaaaat actgtagaag aaagcgacga gaaagccggt
     1861 ttaagagagc gtgtcaaaaa tgcgctgaac gaacttgaga gataacccga aggctgttta
     1921 ctttactagc aatacgcttg cgttcggtgg ttaagtatgt ataatgcgcg ggcttgtcgt
     1981 agttgacagc aggttcaatc tgaacctcag ttagccgaat ttggctacct aacaatgctc
     2041 ccaatcgggg agctacgtaa gaacggttac actctcccat caatcgtaat gggtctgagg
     2101 agtaatcatt ttcgtttata aaataattgg agctctggtc tcatgcagaa ccaaagaatc
     2161 cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc aagcaaccgc ggaaatcgtc
     2221 gagactgcca agcgcactgg tgcgcaggtc cgtggtccga tcccgctgcc gacacgcaaa
     2281 gagcgcttca ctgttctgat ctccccgcac gtcaacaaag acgcgcgcga tcagtacgaa
     2341 atccgtactc acttgcgtct ggttgacatc gttgagccaa ccgagaaaac cgttgatgct
     2401 ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga tcagcctggg ttaatcaggt
     2461 cattgagcga ttgagaggtt gaaacaatga ttggtttagt cggtaaaaaa gtgggtatga
     2521 cccgtatctt cacagaagac ggcgtttcta tcccagtaac cgtaatcgaa gttgaagcaa
     2581 accgcgttac tcaggttaaa gacctggcta acgatggcta ccgtgctatt caggtaacca
     2641 ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc tggccacttc gctaaagctg
     2701 gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc tgaaggcgaa gagttcactg
     2761 taggtcagag cattagcgtt gaactgtttg ctgacgttaa aaaagttgac gtaactggca
     2821 cctctaaagg taaaggtttc gcaggtaccg ttaagcgctg gaacttccgt acccaggacg
     2881 ctactcacgg taactccttg tctcaccgcg ttccgggttc tatcggtcag aaccagactc
     2941 cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat gggtaacgaa cgtgtaaccg
     3001 ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa cctgctgctg gttaaaggtg
     3061 ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc agctgtgaag gcgtaaggag
     3121 atagcaatgg aattagtatt gaaagacgcg cagagcgcgc tgactgtttc cgaaactacc
     3181 ttcggtcgtg atttcaacga agcgctggtt caccaggttg ttgttgctta tgcagctggt
     3241 gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag taactggttc cggtaaaaaa
     3301 ccgtggcgcc agaaaggcac cggccgtgcg cgttctggtt ctatcaagag cccgatctgg
     3361 cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc acagtcaaaa agttaacaag
     3421 aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac tggtacgtca ggatcgtctg
     3481 atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta agctgctggc acagaaactg
     3541 aaagacatgg ctctggaaga tgtgctgatc atcaccggtg agctggacga aaacctgttc
     3601 ctggctgcgc gcaacctgca caaggttgac gtacgcgatg caactggtat cgacccggtt
     3661 agcctgatcg ccttcgacaa agtcgtaatg actgctgatg ctgttaagca agttgaggag
     3721 atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct gcgtgcaccg cacgtttctg
     3781 aaaaagcgtc tactgcgatg gaaaaatcta acaccatcgt actcaaagtt gctaaagacg
     3841 cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt tgaagtcgaa gtcgaagtcg
     3901 ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg acagcgtatc ggtcgtcgta
     3961 gcgactggaa aaaagcttac gtcaccctga aagaaggcca gaatctggac ttcgttggcg
     4021 gcgctgagta agtcggagga gtaatacaat ggcagttgtt aaatgtaaac cgacatctcc
     4081 gggtcgtcgc cacgttgtta aagtggttaa ccctgagctg cacaagggca aaccttttgc
     4141 tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac aacaatggcc gtatcaccac
     4201 tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt gttgacttca aacgcaacaa
     4261 agacggtatc ccggcagttg ttgaacgtct tgagtacgat ccgaaccgtt ccgcgaacat
     4321 cgcgctggtt ttgtacaaag acggtgaacg ccgttacatc ctggccccta aaggcctgaa
     4381 agctggcgac cagattcagt ctggcgttga tgctgcaatc aaaccaggta acaccctgcc
     4441 gatgcgcaac atcccggttg gttctactgt tcataacgta gaaatgaaac caggtaaagg
     4501 cggtcagctg gcacgttccg ctggtactta cgttcagatc gttgctcgtg atggtgctta
     4561 tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta gaagcagact gccgtgcaac
     4621 tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt ctgggtaaag caggtgctgc
     4681 acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg atgaacccgg tagaccaccc
     4741 acatggtggt ggtgaaggtc gtaactttgg taagcacccg gtaactccgt ggggcgttca
     4801 gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat aaattcatcg tacgtcgccg
     4861 tagcaaataa ttttagagga taagccatgc cacgttctct caagaaaggt ccttttattg
     4921 acctgcactt gctgaagaag gtagagaaag cggtggaaag cggagacaag aagcccctgc
     4981 gcacttggtc ccgtcgttca acgatctttc ctaacatgat cggtttgacc atcgctgtcc
     5041 ataatggtcg tcagcacgtt ccggtatttg taaccgacga aatggtcggt cacaaactgg
     5101 gtgaattcgc accgactcgt acttatcgcg gccacgctgc tgataaaaaa gcgaagaaga
     5161 aataaggtag gaggaagaga tggaaactat cgctaaacat cgccatgctc gttcttctgc
     5221 tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag aaagtgtcgc aggctctgga
     5281 tattttgacc tacaccaaca agaaagcggc tgtactggtc aagaaagttc tggaatctgc
     5341 cattgctaac gctgaacaca acgatggcgc tgacattgac gatctgaaag ttacgaaaat
     5401 tttcgtagac gaaggcccga gcatgaagcg cattatgccg cgtgcaaaag gtcgtgcaga
     5461 tcgcatcctg aagcgcacca gccacatcac tgtggttgtg tccgatcgct gagactctgg
     5521 agactagcaa tgggtcagaa agtacatcct aatggtattc gcctgggtat tgtaaaacca
     5581 tggaactcta cttggtttgc gaacaccaaa gaattcgctg acaacctgga cagcgatttt
     5641 aaagtacgtc agtacctgac taaggaactg gctaaagcgt ccgtatctcg tatcgttatc
     5701 gagcgtccgg ctaagagcat ccgtgtaacc attcacactg ctcgcccggg tatcgttatc
     5761 ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg tagcggacat cgctggcgtt
     5821 cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac tggacgcaaa actggttgct
     5881 gacagcatca cttctcagct ggaacgtcgc gttatgttcc gtcgtgctat gaagcgtgct
     5941 gtacagaacg caatgcgtct gggcgctaaa ggtattaaag ttgaagttag cggccgtctg
     6001 ggcggcgcag aaatcgcacg taccgaatgg taccgcgaag gtcgcgtacc gctgcacact
     6061 ctgcgtgctg acatcgacta caacacctct gaagcgcaca ccacttacgg tgtaatcggc
     6121 gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta tggctgctgt tgaacaaccg
     6181 gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag gccgtaaata aggagcgtcg
     6241 ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc acaaaggccg taaccgcggt
     6301 ctggcgcagg gtacggatgt tagcttcggc agcttcggtc tgaaagctgt tggccgtggt
     6361 cgtctgactg cccgtcagat cgaagcagca cgtcgtgcta tgacccgtgc agttaagcgt
     6421 caaggtaaga tctggatccg tgtgttcccg gacaaaccga tcactgaaaa gccgctggca
     6481 gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg ttgccttgat tcagccgggt
     6541 aaagtcctgt atgaaatgga cggtgttccg gaagagctgg cccgtgaagc attcaagctg
     6601 gcagcagcga aactgccgat taaaaccacc tttgtaacta agacggtgat gtaatgaaag
     6661 caaaagagct gcgtgagaag agcgttgaag agctgaacac cgagctgctg aacctgctgc
     6721 gtgagcagtt caacctgcgt atgcaggctg caagtggcca gctgcaacag tctcacctgt
     6781 tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt actgaacgag aaggcgggtg
     6841 cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt gttagcgaca aaatggagaa
     6901 atccattgtt gttgctatcg aacgttttgt gaaacacccg atctacggta aattcatcaa
     6961 gcgtacgacc aaactgcacg tacatgacga gaacaacgaa tgcggtatcg gtgacgtggt
     7021 tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc tggacgctgg ttcgcgttgt
     7081 agagaaagcg gttctgtaat acagtacact ctctcgatac gaataaacgg ctcagaaatg
     7141 agccgtttat tttttctacc catatccttg aagcggtgtt ataatgccgc gccctcgata
     7201 tggggatttt taacgacctg attttcgggt ctcagtagta gttgacatta gcggagcact
     7261 aaaatgatcc aagaacagac tatgctgaac gtcgccgaca actccggtgc acgtcgcgta
     7321 atgtgtatca aggttctggg tggctcgcac cgtcgctacg caggcgtagg cgacatcatc
     7381 aagatcacca tcaaagaagc aattccgcgt ggtaaggtca aaaaaggtga tgtgctgaag
     7441 gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg acggttctgt cattcgcttc
     7501 gatggtaatg cttgtgttct tctgaacaac aacagcgagc agcctatcgg tacgcgtatt
     7561 tttgggccgg taactcgtga gcttcgtagt gagaagttca tgaaaattat ctctctggca
     7621 ccagaagtac tctaaggagc gaatcatggc agcgaaaatc cgtcgtgatg acgaagttat
     7681 cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt aagaatgtcc tgtcttccgg
     7741 caaggtcatt gttgaaggta tcaacctggt taagaaacat cagaagccgg ttccggccct
     7801 gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt caggtttcca acgtagcaat
     7861 cttcaatgcg gcaaccggca aggctgaccg tgtaggcttt agattcgaag acggtaaaaa
     7921 agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa tttggagtag tacgatggcg
     7981 aaactgcatg attactacaa agacgaagta gttaaaaaac tcatgactga gtttaactac
     8041 aattctgtca tgcaagtccc tcgggtcgag aagatcaccc tgaacatggg tgttggtgaa
     8101 gcgatcgctg acaaaaaact gctggataac gcagcagcag acctggcagc aatctccggt
     8161 caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag gcttcaaaat ccgtcagggc
     8221 tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca tgtgggagtt ctttgagcgc
     8281 ctgatcacta ttgctgtacc tcgtatccgt gacttccgtg gcctgtccgc taagtctttc
     8341 gacggtcgtg gtaactacag catgggtgtc cgtgagcaga tcatcttccc agaaatcgac
     8401 tacgataaag tcgaccgcgt tcgtggtttg gatattacca ttaccactac tgcgaaatct
     8461 gacgaagaag gccgcgctct gctggctgcc tttgacttcc cgttccgcaa gtaaggtagg
     8521 gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt aaaacgcgta gctttagctg
     8581 ataaatactt cgcgaaacgc gctgaactga aagcgatcat ctctgatgtg aacgcttccg
     8641 acgaagatcg ttggaacgct gttctcaagc tgcagactct gccgcgtgat tccagcccgt
     8701 ctcgtcagcg taaccgctgc cgtcaaacag gtcgtccgca tggtttcctg cggaagttcg
     8761 ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg tgaaatcccg ggtctgaaaa
     8821 aggctagctg gtaattgtca ccaattgaat cacgggagta aagacagatg agcatgcaag
     8881 atccgatcgc ggatatgctg acccgtatcc gtaacggtca ggccgcgaac aaagctgcgg
     8941 tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa cgtgctgaag gaagaaggtt
     9001 ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga actggaactt actctgaagt
     9061 atttccaggg caaagctgtt gtagaaagca ttcagcgtgt cagccgccca ggtctgcgca
     9121 tctataagcg taaagatgag ctgccgaaag ttatggcggg tctgggtatc gcagttgttt
     9181 ctacctctaa aggtgttatg actgatcgtg cagcgcgcca ggctggtctt ggtggcgaaa
     9241 ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa gcaccggtcg
     9301 ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt tattacgatc aaaggtaaaa
     9361 acggcgagct gactcgtact ctcaacgatg ctgttgaagt taaacatgca gataataccc
     9421 tgaccttcgg tccgcgtgat ggttacgcag acggttgggc acaggctggt accgcgcgtg
     9481 ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt cactaagaag ctgcagctgg
     9541 ttggtgtagg ttaccgtgca gcggttaaag gcaatgtgat taacctgtct ctgggtttct
     9601 ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg actcagactg
     9661 aaatcgtgct gaaaggcgct gataagcagg tgatcggcca ggttgcagcg gatctgcgcg
     9721 cctaccgtcg tcctgagcct tacaaaggca agggtgttcg ttacgccgac gaagtcgtgc
     9781 gtaccaaaga ggctaagaag aagtaaggta acactatgga taagaaatct gctcgtatcc
     9841 gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc ctggtggtac
     9901 atcgtacccc gcgtcacatt tacgcacagg taattgcacc gaacggttct gaagttctgg
     9961 tagctgcttc tactgtagaa aaagctatcg ctgaacaact gaagtacacc ggtaacaaag
    10021 acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa ggcatcaaag
    10081 atgtatcctt tgaccgttcc gggttccaat atcatggtcg tgtccaggca ctggcagatg
    10141 ctgcccgtga agctggcctt cagttctaag gtagaggtgt aagatggctc acatcgaaaa
    10201 acaagctggc gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta aaaccgttaa
    10261 aggtggtcgt attttctcct tcacagctct gactgtagtt ggcgatggta acggtcgcgt
    10321 tggttttggt tacggtaaag cgcgtgaagt tccagcagcg atccagaaag cgatggaaaa
    10381 agcccgtcgc aatatgatta acgtcgcgct gaataacggt actctgcaac accctgttaa
    10441 aggtgttcat acgggttctc gcgtattcat gcagccggct tccgaaggta ccggtatcat
    10501 cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg gttcataacg ttctggctaa
    10561 agcgtatggt tccaccaacc cgattaacgt ggttcgtgca actatcgatg gcctggaaaa
    10621 tatgaattcc ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag aaattctggg
    10681 gaaataaacc atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa tcggtcgtct
    10741 gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt cgtattggtc acaccgtaga
    10801 gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg gtttccttca tggttaaagt
    10861 tgaggagtaa gagatgcgtt taaatactct gtctccggcc gaaggctcca aaaaggcggg
    10921 taaacgcctg ggtcgtggta tcggttctgg cctcggtaaa accggtggtc gtggtcacaa
    10981 aggtcagaaa tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg gtcagatgcc
    11041 tctgtaccgt cgtctgccga aattcggctt cacttctcgt aaagcagcga ttacagccga
    11101 agttcgtctg tctgacctgg ctaaagtaga aggcggtgtt gtagacctga acacgctgaa
    11161 agcggctaac attatcggta tccagatcga gttcgcgaaa gtgatcctgg ctggcgaagt
    11221 cactactccg gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg ctgctatcga
    11281 agctgctggc ggtaaaatcg aggaataagt agcagatggc taaacaaccg ggattagatt
    11341 ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag actgctgttt gttatcggtg
    11401 cgctgattgt gttccgtatt ggctctttta ttccgatccc tggtattgat gccgctgtac
    11461 ttgccaaact gcttgagcaa cagcgaggca ccatcattga aatgtttaac atgttctctg
    11521 gtggtgctct cagccgtgct tctatctttg ctctggggat catgccatat atttcggcat
    11581 cgatcattat ccagcttctg acggtggttc acccaacgtt ggcagaaatt aagaaagaag
    11641 gggagtctgg tcgtcgtaag atcagccagt acacccgcta cggtactctg gtgctggcaa
    11701 tattccagtc gatcggtatt gctaccggtc tgccgaatat gcctggtatg caaggcctgg
    11761 tcattaaccc gggctttgca ttctacttca ccgctgttgt aagtctggtc acaggaacca
    11821 tgttcctgat gtggttgggc gaacagatta ctgaacgagg tatcggcaac ggtatttcaa
    11881 tcattatctt cgccggtatt gtcgcgggac tcccgccagc cattgcccat actatcgagc
    11941 aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt ggttgcagta ttagtatttg
    12001 cagtgacgtt ctttgttgta tttgttgagc gtggtcaacg ccgcattgtg gtaaactacg
    12061 cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag cacacattta ccgctgaaag
    12121 tgaatatggc gggggtaatc ccggcaatct tcgcttccag tattattctg ttcccggcga
    12181 ccatcgcgtc atggttcggg ggcggtactg gttggaactg gctgacaaca atttcgctgt
    12241 atttgcagcc tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc atcttcttct
    12301 gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac agcagataac ctgaagaagt
    12361 ccggtgcatt tgtaccagga attcgtccgg gagagcaaac ggcgaagtat atcgataaag
    12421 taatgacccg cctgaccctg gttggtgcgc tgtacattac ctttatctgc ctgatcccgg
    12481 agttcatgcg tgatgcaatg aaagtaccgt tctactttgg tgggacctca ctgcttatcg
    12541 ttgttgtcgt gattatggac tttatggctc aagtgcaaac tctgatgatg tccagtcagt
    12601 atgagtctgc attgaagaag gcgaacctga aaggctacgg ccgataattg gtcgcccgag
    12661 aagttacgga gagtaaaaat gaaagttcgt gcttccgtca agaaattatg ccgtaactgc
    12721 aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc gaagcataaa
    12781 cagcgccaag gctgattttt tcgcatattt ttcttgcaaa gttgggttga gctggctaga
    12841 ttagccagcc aatcttttgt atgtctgtgc gtttccattt gagtatcctg aaaacgggct
    12901 tttcagcatg gaacgtacat attaaatagt aggagtgcat agtggcccgt atagcaggca
    12961 ttaacattcc tgatcataag catgccgtaa tcgcattaac ttcgatttat ggcgtcggca
    13021 agacccgttc taaagccatc ctggctgcag cgggtatcgc tgaagatgtt aagatcagtg
    13081 agctgtctga aggacaaatc gacacgctgc gtgacgaagt tgccaaattt gtcgttgaag
    13141 gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct gatggatctt ggttgctatc
    13201 gcggtttgcg tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag accaacgcac
    13261 gtacccgtaa gggtccgcgc aaaccgatca agaaataatc ggggtgattg aataatggca
    13321 aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg cgtggctcat
    13381 atccatgctt ctttcaacaa caccatcgtg actatcactg atcgtcaggg taacgcgttg
    13441 ggttgggcaa cagccggtgg ttccggtttc cgtggttctc gcaaatccac tccgtttgca
    13501 gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag aatacggcat caagaatctg
    13561 gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc tctgaacgcc
    13621 gcaggtttcc gcatcactaa cattactgat gtgactccga tccctcataa cggttgtcgt
    13681 ccgccgaaaa aacgtcgcgt ataacgcttc gttttccagg tttgttggag aaagaaaatg
    13741 gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg agggcaccga cttattcctt
    13801 aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc tggccagcac
    13861 ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa gcaaaaagtt
    13921 cgccgtatct atggtgtgct ggagcgtcag ttccgtaact actacaaaga agcagcacgt
    13981 ctgaaaggca acaccggtga aaacctgttg gctctgctgg aaggtcgtct ggacaacgtt
    14041 gtataccgta tgggcttcgg tgccactcgt gcagaagcac gtcagctggt tagccataaa
    14101 gcaattatgg taaacggtcg tgttgttaac atcgcttctt atcaggttag tccgaatgac
    14161 gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc tctggagctg
    14221 gctgagcagc gtgaaaagcc aacctggctg gaagttgatg ctggcaagat ggaaggtacg
    14281 tttaagcgta agccggagcg ttctgatctg tctgcggaca ttaacgaaca cctgatcgtc
    14341 gagctttact ccaagtaaag cttagtacca aagagaggac acaatgcagg gttctgtgac
    14401 agagtttcta aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc acgccaaggt
    14461 gacccttgag cctttagagc gtggctttgg ccatactctg ggtaacgcac tgcgccgtat
    14521 tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg gtgtactaca
    14581 tgagtacagc accaaagaag gcgttcagga agatatcctg gaaatcctgc tcaacctgaa
    14641 agggctggcg gtgagagttc agggcaaaga tgaagttatt cttaccttga ataaatctgg
    14701 cattggccct gtgactgcag ccgatatcac ccacgacggt gatgtcgaaa tcgtcaagcc
    14761 gcagcacgtg atctgccacc tgaccgatga gaacgcgtct attagcatgc gtatcaaagt
    14821 tcagcgcggt cgtggttatg tgccggcttc tacccgaatt cattcggaag aagatgagcg
    14881 cccaatcggc cgtctgctgg tcgacgcatg ctacagccct gtggagcgta ttgcctacaa
    14941 tgttgaagca gcgcgtgtag aacagcgtac cgacctggac aagctggtca tcgaaatgga
    15001 aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca ttctggctga
    15061 acaactggaa gctttcgttg acttacgtga tgtacgtcag cctgaagtga aagaagagaa
    15121 accagagttc gatccgatcc tgctgcgccc tgttgacgat ctggaattga ctgtccgctc
    15181 tgctaactgc cttaaagcag aagctatcca ctatatcggt gatctggtac agcgtaccga
    15241 ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct cttactgaga ttaaagacgt
    15301 gctggcttcc cgtggactgt ctctgggcat gcgcctggaa aactggccac cggcaagcat
    15361 cgctgacgag taaccggatc acaggttaag gttttactga gaaggataag gtcatgcgcc
    15421 atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct atgttccgca
    15481 atatggcagg ttcactggtt cgtcatgaaa tcatcaagac gactctgcct aaagcgaaag
    15541 agctgcgccg cgtagttgag ccgctgatta ctcttgccaa gactgatagc gttgctaatc
    15601 gtcgtctggc attcgcccgt actcgtgata acgagatcgt ggcaaaactg tttaacgaac
    15661 tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg tattctgaag tgtggcttcc
    15721 gtgcaggcga caacgcgccg atggcttaca tcgagctggt tgatcgttca gagaaagcag
    15781 aagctgctgc agagtaatct gaagcaacgt aaaaaaaccc gccccggcgg gtttttttat
    15841 acccgtagca tccccactta tctacaatag ctgtactctt tttgttcatc ccctggagta
    15901 tatatgtggt tacttgacca gtgggcagag cgtcatatag cagaagcgca agcgaaaggt
    15961 gagtttgata acctggcagg tagcggcgaa ccattgatac tggctgatga ttctcatgtg
    16021 ccaccggaat tacgtgcggg gtatcgcttg ctgaagaatg ccggttgctt accgccagaa
    16081 cttgagcagc ggagggaagc aattcagctt ctggatatcc tgagaggtat ccgtcacgat
    16141 gatccgcaat atcaagaggt tagccgtcga ttgtcattac tggaattgaa gctgcgacaa
    16201 gctggactga gcaccgattt tttacgcggc gattatgctg acaagttgtt gaataaaatc
    16261 aacgataact agtggagtat gtatgtatcg cattggtgag ctggcaaaaa tggcggaagt
    16321 aacacccgac acgattcgtt attacgaaaa acagcagatg atggagcatg aagtgcgtac
    16381 tgaaggtggg tttcgcctgt ataccgaaag cgatctccag cgattgaaat ttatccgcca
    16441 tgccagacaa ctaggtttca gtctagagtc gatccgcgag ttgctgtcga tccgtatcga
    16501 tcctgaacac catacctgtc aggagtcaaa aggcattgtg caggaaagat tgcaggaagt
    16561 cgaagcacgg atagccgagt tgcagagtat gcagcgttcc ctccaacgcc ttaacgatgc
    16621 ctgttgtggg accgcccata gcagtgttta ttgctcgatt cttgaagctc ttgaacaagg
    16681 ggcgagtggc gttaagagtg gctgttgatt ttttacactg gcaggattac actcgcgccg
    16741 ttaaataacc aattggagtt tttatgagcc gatatcagca tactaaaggg cagataaagg
    16801 ataatgcgat agaagcatta ctacatgatc ccttattccg acagcgcgta gagagaaata
    16861 agaaagggaa aggcagttac atgcgaaaag gaaaacatgg caatcggggg aactgggagg
    16921 ccagtggcaa gaaagtaaat cactttttta ccactggcct tctgctttca ggcgcttgtt
    16981 aagagcggtt attctgctct ttcagcaaat cacgaatttc tgtcagtaat acttcttctt
    17041 tagttggtgt aggcgcggct gctggttctt cttttttccg attcagtttg ttgattagct
    17101 taatcgccat aaagatggca aaggctacaa tcagaaaatc aaagacgttt tgaatgaaga
    17161 caccgtaatg catcacaaca gcagggatat ccccctgcgc atcgcgtagc gtgacagcaa
    17221 actgtttgaa atcgatcccg ccaattaata accccagagg tggcataatg atatcggcaa
    17281 ccagtgaaga tacaatcttc ccgaatgccg caccgataat gacacccacc gccaaatcca
    17341 ccacgttccc gcgcatcgca aattcgcgaa attctttaat aatgctcatg ttattctccc
    17401 tatgaagccg acaatcataa gtctaacaaa tgttaagcta ttttcctgcc aggagtgaat
    17461 ttaattaatc cctgataatc aaagggaaat aataaggcgt cattagacgc cttattaatt
    17521 acaagaagaa aggacttggc tggaagagtc tttcgacgtc ggtaataaac tttttatctg
    17581 tgaggaacat aattacgtga tcgccttgct caatgcgcag attgtcattg gcaatcatca
    17641 cgtcgtttcc acgtaccacc gctccaataa tcgttcctgg cggtagcttg atttcatcaa
    17701 tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg agcaacggct tcaatagctt
    17761 ctgctacgcc gcggcgcagt gaggaaacac caacaatatc agcttttcgc acatggctaa
    17821 gcaacgcaga aatagttgct tgttgcggtg aaatcgcaat atcgatcacg ctcccctgaa
    17881 ccagatccac ataagcgcga cgctggatca ataccatcac ctttttcgca cccatacgtt
    17941 tggcaagcat ggcggacatg atattggctt cgtcatcgtt ggtgacagca ataaacagat
    18001 caacttgatc gatatgttct tcggccagca gttcttgatc cgacgcatca ccaaaaaaga
    18061 cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg ctgctgatta cgttcgatga
    18121 gtttaacgct gtaatctttt tccagacgac gcgccagccc tgcaccgata ttaccgccgc
    18181 caaccagcat gatccgctta tacggttttt ccagtcgctg taattcactc atcaccgcgc
    18241 ggatatgctg tgaagcggca ataaagaaca cttcatcacc agcttcaaca atggtcgaac
    18301 cttgcggacg aatggggcga tcgtggcgga aaattgctgc cacacgagta tcgatatggg
    18361 gcatatgttc gcgcatggtc gaaagtgcat taccaatcag cgggccgcca taataggctt
    18421 taaccacagc caggctgact ttaccctcag cgaagttcac cacctgcaat gcgccgggat
    18481 actcaatcag tcggtaaata ttatcgataa ccaactgctc tggtgcgatc agatgatcaa
    18541 tcggtacagc atctgaatga aatagtttat cggcatcgcg cacgtagtct ggtgagcgga
    18601 tacgagcgat gcgattaggg gtgttgaaaa gtgagtaggc tacctggcag gcaaccatat
    18661 tggtttcatc tgaactagtt acagcaacca gcatatcggc gtcgtcggca cctgcctccc
    18721 gcaatacgcg tggatgagag ccatgcccct gcacgactcg caggtcaaat ttatcctgca
    18781 aggtccgcag acgctcaccg ttggtatcga caacagtaat atcgttgttc tcgccaacca
    18841 ggttttccgc cagtgtgccg ccaacctggc cggcacccag aatgataatt ttcatcagtc
    18901 gcgacccgtt atctcatcac tttttgatta gcttagcgta aaagaagccg tcgccctctt
    18961 cggcaccagg cagattttgt ttacccggtt gctctggtgt tcctgtttcg caaagttcgg
    19021 catcagcggt acgttgcaaa aaggctttaa tctgcaggct attctcttcc ggtaacaccg
    19081 aacaggtggc atagaccagg gttccaccgg attttaaatg cggccaaatg gcgtcgagaa
    19141 tttcagactg caattgcgcg agttccggga tatcgcgatc gcgacgtaac catttaatat
    19201 ccggatggcg acgaatcaca ccggttgctg aacaaggcgc atctaataaa atgcgatcaa
    19261 actgttgctc gccacaccat tgggaagggt agcggccatc accttgtttc acggttgcct
    19321 tcataccaag gcgttttaaa ttgtcgtaaa cgcgagagag gcgctgttcg tcaatatcaa
    19381 ccgcaacaac ctgcgcttct ggtgccacct caaggatatg cgttgtttta ccgccggggg
    19441 cggcacaaag atccaaaatg tgttcaccgt tttgtggcgc aagccaggtc atgcaacctt
    19501 gtgctgaggc atcctgaacg gtaacccatc cgtcttcaaa accaggtagc gcatgaacag
    19561 gtgcaggtgt ttccagacgt acagcatcag ggtaatccgc aggcgggaaa cctttcattc
    19621 ctgcttcatc cagcaatgca agccagctgt cgcgggaatg atgcgtacga ttaacgcgca
    19681 gccacattgg cggacgctgg ttattggctt cgacgatgga ttgccactgc tctggatacg
    19741 ctttttgcag acgcttcagc aaccaggaag gatgcagata acgtgcatca ctggcattaa
    19801 actcggctaa taactcttct tgctgacgct ggaactggcg taatacaccg tttatcaacc
    19861 ctttaagttg cgggcgctta attgcgatag cgccttcaac cgtttcagcc agcgcagcat
    19921 gaggtggtat gcgggtataa agcagttgat acaaaccaac cataatcagg taatgcacgg
    19981 tccgctgttt gccggtcatc ggacgggcca ttaacttatt aatcagccag tttaactgcg
    20041 aaagcgtacg cagtacgcca aagcacaact cttgaagaag tgctttgtct ttatcggaaa
    20101 ctttttgctg gagcggtggc agaatgttgc ttaatgattg cccttgctcg acgacttgtt
    20161 caacggcctg ggccgccatg ctacgtaaat tacgttgttt tttcataagt ataaaaatgc
    20221 ccggcaagac cgggcttaga agagtggact atcagaccag acggttgccc ggaacaaacc
    20281 attcccgacg agagttcagg aggtcttgcg cgctcatcgc tttcttaccc gcaggttgta
    20341 acgagagcag gttcaggatg ccatcaccag tcgcaacctg aatgccttgt ttgttggctt
    20401 caaggatcgt tcctggtgca gcgttggttg ccgtatcaat gaccgatgct ttccagactt
    20461 taaccggctg tccttcaatt tccagccagc tcattggcca tggattgaaa gcgcgaatgc
    20521 agcgttcaag ctgtgctgcc gaaagtgacc agtcaatacg cgcttcttct ttactcaact
    20581 tctcggcgta agtgacaaga gtttcgtcct gaacttctgg tttcgccgtg ccgtctgcca
    20641 attgtttcaa cgtggtgata agcccttgtg ggccaagctc tgccagcttg tcgtacagcg
    20701 taccactggt atcttctgca gtaatcgggc aggagagctt atagagcatg tctccggtgt
    20761 ctaaaccaac atccatttgc ataatggtca caccagtttc tgcatcaccc gcccatagtg
    20821 agcgttggat tggtgcagca ccgcgccagc gtggcagcag tgaaccatga acgttgatac
    20881 agccaagacg cggcatctcc agcactgctt taggcagaat taagccatag gcgacgacaa
    20941 ccataacatc agcctgtaga tcggcgacca gttgctggtt ttcttgtgga cgcagggaaa
    21001 ccggttgaaa aacgggcaga cctttttcct cagccagaac tttaaccggg ctgggcatca
    21061 gttttttacc gcgtcctgcc ggtcggtctg gctgggtgaa cacgccaacg acgttatgac
    21121 cagaagacaa cagcgcgtcg agatgacgcg ctgcaaagtc aggtgtaccc gcaaaaataa
    21181 tacgtagtga ttctgacacg ttagttctta tccttaagcc cgggctttca gacgatccag
    21241 tttttcaact ttctgacgaa tacgttgttg tttcagcggt gacagataat ccataaacag
    21301 tttgccgacc aggtgatcca tctcatgctg aatacagatg gctaacagac cctctgcttc
    21361 cagttcaaat ggtttaccgt cgcggtccag ggcgcgaatt ttaactttct ctgcgcgtgg
    21421 cactaaagca cgttgttcag ggatcgacag gcatccttct tcaatgcctg tttcgccgct
    21481 tttttctaaa agctccggat tgattaacac cagccgttcg tcacggtttt ccgaaacatc
    21541 aataacaatg atacgttgat ggatatcgac ctgggttgcc gccaggccaa taccttcttc
    21601 tgcgtacatc gtctcgaaca tatcatcgac gatacgctga atttctgcat tcacttcttc
    21661 taccggttta gcaactttgc gaagccgctc gtccggaata tgtaacactt gcaaaactga
    21721 cataaatctc cagagatgtg ttcaggagtt agaaagatta tttcttctat tctagacaaa
    21781 tccccctctg attgacagca tcactgacca atcgcaaaga ttgctaaggc tgcttatggc
    21841 agggagataa ggatggtcga tacagaaatt tggctgcgtt taatgagcat cagcagcttg
    21901 tacggcgatg atatggtccg tatagctcac tggctggcaa aacagtcgca tattgatgcg
    21961 gttgtattgc agcaaactgg gcttacattg cggcaggcac aacgctttct ttcatttccg
    22021 cgaaagagta tcgaaagctc actttcttgg ttggagcaac ccaaccatca tttaattcct
    22081 gcggacagcg aattttatcc tcctcaactt cttgcgacga cagattaccc cggcgcactg
    22141 tttgttgaag gagaactgca cgcgctgcat tcatttcagc ttgccgtagt ggggagtcgg
    22201 gcgcattcat ggtatggcga gcgatgggga cgattatttt gcgaaactct ggcgacgcgt
    22261 ggagtgacaa ttacgagtgg actggcgcgt ggaatcgatg gtgtggcgca taaagcggcc
    22321 ttacaggtaa atggcgtcag cattgctgta ttggggaatg gacttaatac cattcatccg
    22381 cgtcgccatg cccgactggc taccagtttg cttgaacatg gtggggctct tgtctcggaa
    22441 tttcccctcg atgttccacc ccttgcttac aatttcccac gaagaaatcg cattatcagt
    22501 ggtctaagta aaggtgtact ggtggtggaa gcggctttgc gcagtggttc gctggtgaca
    22561 gcacgttgtg cgcttgagca ggggcgtgaa gtttttgcct tgccgggtcc attagggaat
    22621 ccgggaagcg aagggcctca ctggttaata aaacaaggtg cgattcttgt gacggaaccg
    22681 gaagaaattc tggaaaactt gcaatttgga ttgcactggt tgccagacgc ccctgaaaat
    22741 tcattttatt caccagatca ggaagacgtg gcattgccat ttcctgagct cctggctaac
    22801 gtaggagatg aggtaacacc tgttgacgtc gtcgctgaac gtgccggcca acctgtgcca
    22861 gaggtagtaa ctcaactact cgaactggag ttagcaggat ggatcgcagc tgtacccggc
    22921 ggctatgtcc gattgaggag ggcatgccat gttcgacgta ctaatgtatt tgtttgaaac
    22981 ctatattcac acagaagctg agttgcgtgt ggatcaagac aaacttgaac aggatcttac
    23041 cgacgcagga tttgatcgag aagatatcta caatgccctg ctttggctgg aaaagcttgc
    23101 ggattatcag gaaggactgg cagaaccgat gcaactggcc tctgatccac tctccatgcg
    23161 tatttatacc cctgaagagt gtgaaaggct ggatgccagc tgtcgtggtt ttctgctttt
    23221 ccttgagcaa attcaggtgc ttaaccttga aacccgtgaa atggtgatag agcgagtgct
    23281 cgcgctggat accgcagagt tcgacctgga agacctgaaa tgggtaatcc tgatggtgtt
    23341 gttcaatatt ccgggctgcg aaaatgcgta ccagcaaatg gaagaattac tctttgaagt
    23401 gaatgaaggt atgctgcatt aatcatttct ttaattcagc ataagttgtt atggcgaaat
    23461 cagcactgtt cacggtgcgt aataatgagt cctgcccaaa gtgcggggct gaactggtta
    23521 ttcgatccgg gaaacacggt ccgtttcttg gatgctcaca gtatccggcg tgtgactacg
    23581 tccgtcctct gaaatcttca gcggatggac atattgtcaa agttctggag gggcaggttt
    23641 gccctgcatg tggcgcaaat ctggtattac gccagggacg ctttggtatg tttattggtt
    23701 gcagtaacta ccctgaatgc gaacataccg aacttatcga taaaccggac gaaacagcaa
    23761 ttacctgtcc ccaatgtcgg acgggccatc tggtccagcg ccgttcccgt tatggcaaaa
    23821 catttcactc ctgtgatcgc tacccggagt gtcaatttgc cattaacttc aaacccatag
    23881 ctggagaatg ccctgagtgt cattatccgc tactcatcga aaagaaaacc gcgcagggtg
    23941 taaaacactt ttgtgccagt aaacaatgtg gaaagccggt ttcggcggaa taataacgtg
    24001 aataataacc tgcaaggaga cgctatcgca gctgcgatag atgtcctcaa tgaaaaacgt
    24061 gtcatcgcct atccaacgga agccgttttc ggtgtcgggt gcgatcctga tagcgaaaca
    24121 gcagtgatgc gactgttgga gttaaaacag cgtccggttg ataaggggct gattttaatc
    24181 gcggcaaatt acgagcagct taaaccctat attgatgaca ccatgctgac tgatgcgcag
    24241 cgtgaaacca ttttttcccg ctggccaggt cctgtcacct ttgtctttcc cgcgcctgcg
    24301 acaacaccgc gctggttgac gggccgcttt gattcgcttg ctgtacgagt caccgaccat
    24361 ccgttggtgg ttgctttgtg ccaggcttat ggtaaaccgc tggtttctac cagtgccaac
    24421 ttgagtggat tgccaccttg tcgaacagta gacgaagttc gcgcacaatt tggcgcggcg
    24481 ttcccggttg tgcctggtga aacggggggg cgtttaaatc cttcagaaat ccgcgatgcc
    24541 ctgacgggtg aactgtttcg acaggggtaa cataatggaa acctatgctg tttttggtaa
    24601 tccgatagcc cacagcaaat cgccattcat tcatcagcaa tttgctcagc aactgaatat
    24661 tgaacatccc tatgggcgcg tgttggcacc catcaatgat ttcatcaaca cactgaacgc
    24721 tttctttagt gctggtggta aaggtgcgaa tgtgacggta ccttttaaag aagaggcttt
    24781 tgccagagcg gatgagctta ctgaacgggc agcgttggct ggtgctgtta ataccctcaa
    24841 gcggttagaa gatggacgcc tgctgggtga caataccgat ggtataggtt tgttaagcga
    24901 tctggaacgt ctgtctttta tccgtcctgg tttacgtatt ctgctcatcg gcgctggtgg
    24961 agcttctcgc ggcgtactac tgccacttct ttccctggac tgtgcggtga caatcactaa
    25021 tcggacggta tcccgcgcgg aagagttggc taaattgttt gcgcacactg gcagtattca
    25081 ggcgttgggt atggacgaac tggaaggtca tgagtttgat ctcattatta atgcaacatc
    25141 cagtggcatc agtggtgata ttccggcgat cccgtcatcg cttattcatc caggcattta
    25201 ttgctatgac atgttctatc agaaaggaaa aactcctttt ctggcatggt gtgagcagcg
    25261 aggctcaaag cgtaatgctg atggtttagg aatgctggtg gcacaggcgg ctcatgcctt
    25321 ccttctttgg catggcgttc tgcctgatgt agaaccggtc atcaaactat tgcaacagga
    25381 attgtcagcg tgaatcaggc catccagttt ccggataggg aagagtggga cgagaataaa
    25441 agatgtgtat gttttcccgc tctcgtgaat ggtatgcaac tgacatgtgc gatctctggc
    25501 gagagtctgg cgtatcgctt tactggagat acgccagaac agtggttagc gagttttcgt
    25561 cagcatcgct gggacctgga agaagaagcg gaaaacttaa ttcaggaaca aagtgaagat
    25621 gatcaaggtt gggtctggtt accctgatcc agatattcgt ccttccattt cacgtaattc
    25681 ttcgcggaat agcgtaaccc agccttctct tcatcactta acgggcggat ctgtttgacg
    25741 gggctaccga gatacagata tccgctctcc agccgtttat tttgtgggac caggctaccc
    25801 gcaccaatca tcacatcatc ttctactatt gcgccatcaa gtaaaattga gcccatccca
    25861 accaaaactc gattgccaat ggtgcagccg tggagcatca ccttgtgacc aacagtgaca
    25921 tcttcgccaa tggttaatgg gttgccatct gggttgtacg aggatttatg agtgacatgc
    25981 aacatactgc catcctggat attggtgcgt gctccgatct gtacataatg tacatctcca
    26041 cgaatcacaa cgagcggcca gatccccaca tcatcagcca gacgaacgtc accaatcacg
    26101 acgctgctat cgtcgatcat tacgcgctgg ccgatttgtg gaaaaagatc gcggtatggg
    26161 cgtaaaacat cagacatact cacctcaaca ataaatgatt tactaatgac tttgggtgca
    26221 ttattggcct tgtgcaagtc ttttagcatg caaaaaagca ccgttttgtg tgcgaatgca
    26281 gcaaaaaggg tgaaaaagca acaaacagaa aaaaagatca aaaaagtact tgtgcaaaaa
    26341 attgggatcc ctataatgcg cctccgttga gacgacaacg tgaaacactt cacaagatgg
    26401 tcgtaacaac aaagagaaaa aatcctgaaa ttcggggttg actctgaaag aggaaagcgt
    26461 aatatacgcc acctcgcgac agtgagctga aagccgcg
//
LOCUS       DAZUCC010000050        26357 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZUCC010000050 DAZUCC010000000
VERSION     DAZUCC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.050"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            222..1697
                     /gene="zwf"
                     /locus_tag="ACHY09_001829"
     CDS             222..1697
                     /gene="zwf"
                     /locus_tag="ACHY09_001829"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS3884809.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            1932..3743
                     /gene="edd"
                     /locus_tag="ACHY09_001830"
     CDS             1932..3743
                     /gene="edd"
                     /locus_tag="ACHY09_001830"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFS3884810.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            3780..4421
                     /gene="kdgA"
                     /locus_tag="ACHY09_001831"
                     /gene_synonym="eda"
     CDS             3780..4421
                     /gene="kdgA"
                     /locus_tag="ACHY09_001831"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS3884811.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4477..5655)
                     /gene="purT"
                     /locus_tag="ACHY09_001832"
     CDS             complement(4477..5655)
                     /gene="purT"
                     /locus_tag="ACHY09_001832"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFS3884812.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFREAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGIQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAGQVKVQG
                     "
     gene            5789..6079
                     /gene="yebG"
                     /locus_tag="ACHY09_001833"
     CDS             5789..6079
                     /gene="yebG"
                     /locus_tag="ACHY09_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFS3884813.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            6146..6502
                     /gene="yebF"
                     /locus_tag="ACHY09_001834"
     CDS             6146..6502
                     /gene="yebF"
                     /locus_tag="ACHY09_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFS3884814.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQPDPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            6829..7488
                     /gene="yebE"
                     /locus_tag="ACHY09_001835"
     CDS             6829..7488
                     /gene="yebE"
                     /locus_tag="ACHY09_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFS3884815.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            7697..9757
                     /gene="ptrB"
                     /locus_tag="ACHY09_001836"
     CDS             7697..9757
                     /gene="ptrB"
                     /locus_tag="ACHY09_001836"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFS3884816.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(9754..10416)
                     /gene="exoX"
                     /locus_tag="ACHY09_001837"
     CDS             complement(9754..10416)
                     /gene="exoX"
                     /locus_tag="ACHY09_001837"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFS3884817.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(10440..11096)
                     /gene="yobB"
                     /locus_tag="ACHY09_001838"
     CDS             complement(10440..11096)
                     /gene="yobB"
                     /locus_tag="ACHY09_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFS3884818.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVSAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(11198..11428)
                     /gene="holE"
                     /locus_tag="ACHY09_001839"
     CDS             complement(11198..11428)
                     /gene="holE"
                     /locus_tag="ACHY09_001839"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFS3884819.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            11567..11941
                     /gene="yobA"
                     /locus_tag="ACHY09_001840"
     CDS             11567..11941
                     /gene="yobA"
                     /locus_tag="ACHY09_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFS3884820.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            11945..12817
                     /gene="copD"
                     /locus_tag="ACHY09_001841"
     CDS             11945..12817
                     /gene="copD"
                     /locus_tag="ACHY09_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFS3884821.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGIWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLMLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            12830..13171
                     /gene="yebY"
                     /locus_tag="ACHY09_001842"
     CDS             12830..13171
                     /gene="yebY"
                     /locus_tag="ACHY09_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFS3884822.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            13566..14222
                     /gene="pphA"
                     /locus_tag="ACHY09_001843"
     CDS             13566..14222
                     /gene="pphA"
                     /locus_tag="ACHY09_001843"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFS3884823.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWFMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGIKGADHFWFGHTPLRHRVDIGNLHYIDIGAVFGGELTLVQLQ
                     "
     gene            complement(14223..14414)
                     /gene="yebW"
                     /locus_tag="ACHY09_001844"
     CDS             complement(14223..14414)
                     /gene="yebW"
                     /locus_tag="ACHY09_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFS3884824.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14519..14755)
                     /gene="yebV"
                     /locus_tag="ACHY09_001845"
     CDS             complement(14519..14755)
                     /gene="yebV"
                     /locus_tag="ACHY09_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFS3884825.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(14873..16312)
                     /gene="rsmF"
                     /locus_tag="ACHY09_001846"
     CDS             complement(14873..16312)
                     /gene="rsmF"
                     /locus_tag="ACHY09_001846"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFS3884826.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(16392..19025)
                     /gene="yebT"
                     /locus_tag="ACHY09_001847"
     CDS             complement(16392..19025)
                     /gene="yebT"
                     /locus_tag="ACHY09_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFS3884827.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(18994..20277)
                     /gene="yebS"
                     /locus_tag="ACHY09_001848"
     CDS             complement(18994..20277)
                     /gene="yebS"
                     /locus_tag="ACHY09_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFS3884828.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPTTRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            20407..20904
                     /gene="msrC"
                     /locus_tag="ACHY09_001849"
     CDS             20407..20904
                     /gene="msrC"
                     /locus_tag="ACHY09_001849"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFS3884829.1"
                     /translation="MNKIEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            21001..21699
                     /gene="proQ"
                     /locus_tag="ACHY09_001850"
     CDS             21001..21699
                     /gene="proQ"
                     /locus_tag="ACHY09_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFS3884830.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            21719..23767
                     /gene="prc"
                     /locus_tag="ACHY09_001851"
     CDS             21719..23767
                     /gene="prc"
                     /locus_tag="ACHY09_001851"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFS3884831.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQTFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            23959..24840
                     /gene="htpX"
                     /locus_tag="ACHY09_001852"
     CDS             23959..24840
                     /gene="htpX"
                     /locus_tag="ACHY09_001852"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFS3884832.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(24886..26259)
                     /gene="yebQ"
                     /locus_tag="ACHY09_001853"
     CDS             complement(24886..26259)
                     /gene="yebQ"
                     /locus_tag="ACHY09_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3884833.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPIILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT         6728 a         6525 c         6836 g         6268 t
ORIGIN      
        1 ttattttttt tcataacatg atcagtgtca gatttttacc caatggaaaa cgatgatttt
       61 tttatcagtt ttgccgcact ttgcgcgctt ttaccgtaat cgcacgggtg gataagcgtt
      121 tacagttttc gcaagctcgt aaaagcagta cagtgcaccg taagaaaatt acaagtatac
      181 cctggcttaa gtaccgggat agttaactta aggagaatga catggcggta acgcaaacag
      241 cccaggcctg tgacctggtc attttcggcg cgaaaggcga ccttgcgcgt cgtaaattgc
      301 tgccttccct gtatcaactg gaaaaagccg gtcagctcaa cccggacacc cggattatcg
      361 gcgtagggcg tgctgactgg gataaagcgg cttataccaa agttgtccgc gaggcgctcg
      421 aaactttcat gaaagaaacc attgatgaag gtttatggga caccctgagt gcacgtctgg
      481 atttttgtaa tctcgatgtc aatgacactg ctgcattcag ccgtctcggc gcgatgctgg
      541 atcaaaaaaa tcgtatcacc attaactact ttgccatgcc gcccagcact tttggcgcaa
      601 tttgcaaagg gcttggtgag gcaaaactga atgctaaacc ggcacgcgta gtcatggaga
      661 aaccgctggg gacgtcgctg gcgacctcgc aggaaatcaa cgatcaggtt ggcgaatact
      721 tcgaggagtg ccaggtttac cgtatcgacc actatcttgg taaagaaacg gtgctgaacc
      781 tgttggcgct gcgttttgct aactccctgt ttgtgaataa ctgggacaat cgcaccattg
      841 atcatgttga gattaccgtg gcagaagaag tggggatcga agggcgctgg ggctattttg
      901 ataaagccgg tcagatgcgc gacatgatcc agaaccacct gctgcaaatt ctttgcatga
      961 ttgcgatgtc tccgccgtct gacctgagtg cagacagcat ccgcgatgaa aaagtgaaag
     1021 tactgaagtc tctgcgccgc attgaccgct ccaacgtgcg cgaaaaaacc gtacgcgggc
     1081 aatatactgc gggcttcgcc cagggcaaaa aagtgccggg atatctggaa gaagagggcg
     1141 cgaacaagag cagcaataca gaaaccttcg tggcgatccg cgtcgacatt gataactggc
     1201 gctgggccgg tgtgccattc tacctgcgta ctggtaaacg tctgccgacc aaatgttctg
     1261 aagtcgtggt ctatttcaaa acacctgaac tgaatctgtt taaagagtcg tggcaggatc
     1321 tgccgcagaa taaactgact atccgtctgc aacctgatga aggcgtggat atccaggtac
     1381 taaataaagt tcctggcctt gaccataaac ataacctgca aatcaccaag ctggatctga
     1441 gctattcaga aacctttaat caaacgcatc tggcagatgc ctatgaacgt ctgctgctgg
     1501 aaaccatgcg tggtattcag gcactgttcg tacgtcgcga tgaagtggaa gaagcctgga
     1561 aatgggtaga ctccattact gaggcgtggg cgatggacaa tgatgcgccg aaaccgtatc
     1621 aggccggaac ctggggaccc gttgcctcag tggcgatgat tacccgtgat ggtcgttcct
     1681 ggaatgagtt tgagtaatat ctgcgctcat cctttatggt tattttaccg gtaacatgat
     1741 cttgcacaga ttgtagaaca atttttacac tttcaggcct cgtgcggatt cacccacgag
     1801 gcttttttta ttacactgac tgaaacgttt ttgccctatg agctccggtt acaggcgttt
     1861 cagtcatgaa tcctctgaat gaaacgtgtt gtgaatcatc ctgctctgac aactcaattt
     1921 caggagcctt tatgaatcca caattgttac gcgtaacaaa tcgaatcatt gaacgttcgc
     1981 gcgagactcg ctctgcttac ctcgcccgga tagaacaagc gaaaacttcg accgttcatc
     2041 gttcgcagtt ggcatgcggt aacctggcac acggtttcgc tgcctgccag ccagaagaca
     2101 aagcctcttt gaaaagcatg ttgcgtaaca atatcgccat catcacctcc tataacgaca
     2161 tgctctccgc gcaccagcct tatgaacact atccagaaat cattcgtaaa gctctgcatg
     2221 aagcaaatgc ggttggtcag gttgcaggcg gtgttccggc gatgtgtgat ggtgtcaccc
     2281 aggggcagga tggaatggaa ttgtcgctgt taagccgcga agtgattgcg atgtctgcgg
     2341 cggtggggct gtcccataac atgtttgatg gtgctctgtt cctcggtgtg tgcgacaaga
     2401 ttgtcccggg tctgacgatg gcagccctgt cgtttgggca tttgcctgcg gtgtttgtgc
     2461 cgtctggacc gatggcaagc ggtttgccaa ataaagaaaa agtgcgtatt cgccagcttt
     2521 atgccgaagg taaagtggac cgcatggcac tactggagtc agaagccgcg tcttatcatg
     2581 cgccgggaac atgtactttc tacggtactg ccaacaccaa ccagatggtg gtggagttta
     2641 tggggatgca gttgccaggc tcttcttttg ttcatccgga ttctccgctg cgcgatgctt
     2701 tgaccgctgc agctgcgcgc caggttacgc gcatgaccgg taatggtaat gaatggatgc
     2761 cgatcggtaa gatgatcgat gagaaagtgg tggtgaacgg tatcgttgca ctgctggcga
     2821 ccggtggttc cactaaccac accatgcacc tggtggcgat ggcgcgcgca gccggtattc
     2881 agattaactg ggatgacttc tctgaccttt ctgatgttgt accgctgatg gcacgtctct
     2941 acccgaacgg tccggccgat attaaccact tccaggcggc aggtggcgta ccggttctgg
     3001 tgcgtgaact gctcaaagca ggcctgctgc atgaagatgt caatacggtg gcaggctttg
     3061 gtctgtctcg ttataccctt gaaccatggc tgaataatgg tgaactggac tggcgggaag
     3121 gggcggaaaa atcactcgac agcaatgtga tcgcttcctt cgaacaacct ttctctcatc
     3181 atggtgggac aaaagtgtta agcggtaacc tgggccgtgc ggttatgaaa acctctgccg
     3241 tgccggtaga gaaccaggtg attgaagcgc ctgcggttgt ttttgaaagc cagcatgacg
     3301 ttatgccggc ctttgaagcg ggtttgctgg accgcgattg tgtcgttgtt gtccgtcatc
     3361 aggggccaaa agcgaacgga atgccagaat tacataaact catgccgcca cttggtgtat
     3421 tattggaccg gtgtttcaaa attgcgttag ttactgatgg acgactctcc ggcgcttcag
     3481 gtaaagtgcc gtcagctatc cacgtaacac cagaagccta cgatggcggg ctgctggcaa
     3541 aagtgcgcga cggggacatc attcgtgtga atggacagac aggcgaactg acgctgctgg
     3601 tagacgaagc ggaactggct gctcgcgaac cacacattcc tgacctgagc gcgtcacgcg
     3661 tgggaacagg acgtgaatta ttcagcgcct tgcgtgaaaa actgtccggt gccgaacagg
     3721 gcgcaacctg tatcactttt taagacgaca aatttgtaat caggcgagag aaaactctga
     3781 tgaaaaactg gaaaacaagt gcagaatcaa tcctgaccac cggcccggtt gtaccggtta
     3841 tcgtggtaaa aaaactggaa cacgcggtgc cgatggcaaa agcgttggtt gctggtgggg
     3901 tgcgcgttct ggaagtgact ctgcgtaccg agtgcgcagt tgacgctatc cgtgctatcg
     3961 ccaaagaagt gcctgaagcg attgtgggtg ccggtacggt gctgaatcca cagcagctgg
     4021 cagaagtcac tgaagcgggt gcacagtttg caattagccc gggtctgacc gagccgctgc
     4081 tgaaagctgc taccgaaggg actattcctc tgattccggg gatcagcact gtttccgaac
     4141 tgatgctggg tatggactac ggtttgaaag agttcaaatt cttcccggct gaagctaacg
     4201 gcggtgtgaa agccctgcag gcgatcgcgg gtccgttctc ccaggtccgt ttctgcccga
     4261 caggtggtat ttctccggct aactaccgtg actacctggc gctgaaaagc gtgctgtgca
     4321 tcggtggttc ctggctggtt ccggcagatg cgctggaagc gggcgattac gaccgcatta
     4381 ctaagctggc gcgtgaagct gtagaaggcg ctaagctgta aaagtcaaaa tgcccgatcg
     4441 aggatcgggc atttttgtag cgtttttttg ccgggtttaa ccctgtactt ttacctgtcc
     4501 ggcggcgtgc tttgcgcgct caatcgcttc atcaacactc tcagcggtcg ccagcgcaac
     4561 acccagacga cggctgccat caatttccgg cttaccaaat aaacgaatct gtatgcctgc
     4621 gcctacggca ttctgcacat tatcaaacgt gacattctga ctggtcagtt gtggcaggat
     4681 aacggcagaa gctgcaggac catactgacg gatcccgcca accggaagtc cgagaaaggc
     4741 tcgcacatgc agggcaaact ccgagagatc ctgagaaatt aacgtcacca tcccggtatc
     4801 atgtgggcga ggggagacct cactgaaaat cacctcatca ccacagacaa acagctcgac
     4861 accaaacagc ccataaccgc ccagtgccag caccacttta cgggcaatct cttgcgcacg
     4921 ttccaaagcc agctggctca tttgctgtgg ttgccaggat tcacgatagt cgccatcttc
     4981 ctggcgatga cctaccggtg cacagaaatg gacgccatcc accgcgctga cggttagcag
     5041 ggtaatttcg aagtcaaact taacgacgcc ttcaacaatt acgcggcccg ctccggcgcg
     5101 accgccttgc tgagcgtact cccatgcatg agcaagttgc tctgcagaac gaataaacgt
     5161 ctgccccttg ccggaggaac tcatcaccgg tttaacaatg cagggatagc cgatttcagc
     5221 aaccgcctcg cggaaaaggt tttcgttatc ggcaaaacga taagtggaag tgggcagctg
     5281 cagctcttct gctgccaggc gacggatacc ctcgcgattc atcgttaatt tcgttgcgcg
     5341 agcgcagggg acaacattca gtccttcctc ttcaagttgg atcagcatat cggtggcaat
     5401 agcttcgatc tccggcacga tataatgtgg tttttccagt tcaaccacac ggcgtaatgc
     5461 atcaccatca agcatattaa tgacatggga gcgatgcgcg acatgcatgg ctggtgcgtc
     5521 ggcatagcga tcgacggcaa tcacctctac gccgagacgc tgacactcga ttgccacttc
     5581 tttacccagt tcaccggagc ctaataacat cacgcgagtt gctgccggac gcagcgcagt
     5641 gcctaataac gtcataacga atatcccctg atttattccg cgcgcagtat aaacgaaaac
     5701 gtttgcgcgt gtctttattg ttgatgttga tttcccaatc gagaagaaat atactgtata
     5761 aaatcacagt tattatgaga ggctgcaaat ggcggttgaa gttaaatacg tagtcattcg
     5821 tgagggcgaa gagaaaatgt cgtttaccag caaaaaggaa gccgatgctt atgacaaaat
     5881 gcttgatacg gcggatctgc ttgacacctg gctgacaaat tctccagtgc aaatggaaga
     5941 cgagcaacgt gaagcccttt cgctatggct ggcagaacaa aaagatgtgc tgagcaccat
     6001 tctgaaaacc ggcaaattac cgtccccaca ggtcgtcggt gctgaatccg aagaggaaga
     6061 tgcctcacac gcggcctgaa gcaacttgcg ctcctgacaa tttgtaccat gctttctcct
     6121 gaatcattgt gctatggaga aaaacatgaa aaaaagaggg gcgtttttag ggctgttgtt
     6181 ggtttctgcc tgcgcatcag ttttcgctgc caataatgaa accagcaagt cggtcacttt
     6241 cccaaagtgt gaaggtctgg atgctgccgg aattgccgcg agcgtaaaac gtgattacca
     6301 acaaaatcgc gtggcgcgct gggctgatga tcaaaaaatt gtcggtcagc ccgatcccgt
     6361 ggcatgggtc agtttgcagg acattcaggg gaaagatgat aaatggtcag taccgctaac
     6421 cgtacgtggt aaaagtgccg atattcatta ccaggtcagc gtggactgca aagcgggaat
     6481 ggcggaatat cagcggcgtt aatggcttct gataattcga ttgaccgttg gcggatacgg
     6541 cgcgagtgcc ttgtccgccc tacaacgcaa cacaatcgta gtcctgagaa gacgcagcag
     6601 cgtcgaaccg ggcaatcagc atcaaacagt tccactcaaa acgtcaaact actttcacct
     6661 aaaataccct tatcacctta tatacagtga acaccttacc tggcgcgaat ttctttgaat
     6721 aacggcctac cgccccgctt atttgcgcat cttttgtctc aatatacatt ttctgacatc
     6781 ctcatccgct atgctacagg cactaaaaat ggttattgag gagcaaatat ggctaactgg
     6841 ttaaatcaac tgcaatccct gcttgggcaa agccgttctt ctacctcctc gtctgcggat
     6901 cagggattgg gcaaactttt agtgcctggc gcattaggcg gactggctgg gctgctggtc
     6961 gcaaataaat cagcacgtaa acttcttacg aaatatggca caaacgcgtt actggttggc
     7021 ggcggagcgg tagcgggtac ggtgctgtgg aataaataca aagataaaat tcgcgcggcg
     7081 catcaggatg aaccgcagtt tggcgcgcaa agtacgccgc tggatgagcg tacggaacgt
     7141 ttgatccttg cgctggtctt tgccgctaaa agtgatggcc atattgatgc caaagaacgt
     7201 gcggcaatcg accagcaatt gcgagaagcc ggtgtggaag agaaggggcg tgtactcatt
     7261 gagcaggcaa tcgaacaacc gctggatcca caacgcctgg ctaccggggt ccgcaatgaa
     7321 gaagaggcgc tggaaatcta tttcctgagc tgcgcggcta ttgatattga ccattttatg
     7381 gaacgcagtt acctgaatgc actgggtgat gcgctgaaaa tcccccagga tgttcgtgaa
     7441 ggcattgaac gcgatctcga acagcaaaaa cgtactttgg cggagtaatt ccgctctctg
     7501 gatagcctga agctgattat ttcgggttat ccagaaccgc acagccattt gagcaggctg
     7561 attttctctg tctgcataac attcgatatc cgcgccatta cctgtcatca tctaagcaat
     7621 gactaccctg tttcgcttgc atccccggtg agttttgcca cccttataag atgtttcaac
     7681 cagaaagaac aataacatgc taccaaaagc cgcccgcatt ccccacgcca tgacgcttca
     7741 tggcgatacg cgcatcgata attactactg gctgcgggac gatacgcgtt ctcagccgaa
     7801 agtcctggac tatctgcaac aagaaaatag ttacggtcat cgggtgatgg cctcgcaaca
     7861 agccttgcag gatcgcatct taaaggaaat catcgaccgc attccgcaac gagaagtttc
     7921 tgcgccctac atcaaaaatg gctaccgcta tcggcatatt tatgaaccag gctgtgaata
     7981 tgctatctac cagcgtcaat cggcgttcag tgaagagtgg gacgagtggg aaacattgct
     8041 cgatgccaac aagcgcgcgg ctcatagtga gttttattcg atgggcggaa tggcgattac
     8101 gccagataac accattatgg cactggcaga agattttctt tcccgacggc agtatggcat
     8161 tcgttttcgt aatctggaaa caggtaactg gtacccggaa ctgctggata acgttgaacc
     8221 cagctttgtc tgggcaaatg actcctggac tttctactat gttcgcaagc atccagtgac
     8281 gctgctgcct tatcaggtct ggcgtcacgc catcggtacg ccagcatcgc aagataaact
     8341 gatttacgaa gaaaaagacg atacctatta cgtcagcctg cataaaacga cctcgaagca
     8401 ttatgtggtc attcatctgg ccagcgccac caccagtgaa gttcgcctgc tggacgcgga
     8461 aatggccgat gccgagccgt ttgtttttct gcctcgccgc aaagatcacg aatacagcct
     8521 tgatcactac cagcatcggt tttatctgcg ttccaaccgc cacggtaaaa actttggctt
     8581 ataccgtacc cgtatgcgtg atgagcaaca gtgggaagag ttaattccgc cacgtgataa
     8641 cattatgctg gaagggttta cgctgtttac cgactggctg gtggttgaag agcgtcagcg
     8701 cgggttaacc agtttgcgcc aaatcaaccg caagacccgg gaagtcattg gcattgcctt
     8761 tgatgatccg gcctatgtga cctggattgc ctacaatcca gaacctgaaa ccgcgcgatt
     8821 gcgttatggt tattcttcca tgaccacacc agatactttg tttgaactgg atatggatac
     8881 cggtgagcgt cgtgtattaa aacaaacgga agttcctggt tttgatgcgg cgaattaccg
     8941 cagtgaacac ctgtggatag tcgcccgtga tggcgtcgaa gttccggttt cgctggtcta
     9001 tcatcgcaaa cattttcgca aaggacacaa cccgctgctg gtgtatggct acggttctta
     9061 cggcgcaagt attgatgccg atttcagttt tagccgcttg agtttgttag atcgtggctt
     9121 tgtctacgcc attgtccatg ttcgcggcgg tggtgagctg gggcaacaat ggtacgaaga
     9181 cggtaaattt ctgaagaaga aaaatacgtt taatgattat cttgatgcct gcgatgcatt
     9241 gttaaaactg ggctatggct ctccttcgct ctgttatgcg atgggcggaa gtgcgggggg
     9301 catgttgatg ggcgttgcaa ttaatcaacg cccggaatta ttccacggcg ttatcgccca
     9361 ggtaccgttt gttgatgttg taacaacaat gcttgatgaa tcaattcctc ttaccactgg
     9421 tgagtttgaa gagtggggga atccgcagga tccgcaatat tacgagtaca tgaaaagcta
     9481 cagcccatat gacaacgtca ccgcacaggc ttatccgcat ttactggtaa cgaccggttt
     9541 gcacgattct caggtgcaat attgggaacc ggcaaaatgg gtcgctaaat tgcgcgagct
     9601 gaaaaccgat gaccatcttt tattgctctg taccgacatg gactcaggtc atggcggtaa
     9661 atctggtcgc tttaaatcgt acgaaggcgt agcgatggaa tatgcttttc tggtcgcgct
     9721 ggcgcaggga acattacccg ctacgcctga ggattaagta ttttccagat aatgtttcag
     9781 tgttaaacgc agctccgggc tcatgctgtc caggttatta aacaaccagc gcagatagcc
     9841 cggatcgcgt tcggcaacgt cggaaaccgc tttgccacgg tatttgccaa aggtgaaggt
     9901 cgtcatcaac gacggacgtc cggtgatatc ggccatctgt tctgccgtcc agccggaggt
     9961 gttcataata tcgataagca acgcagcggt gatataacag tcatacaacg cgcggtgatg
    10021 atgcaggccc ggcggcgtct gtacattgag cttccgtgtt ttatataacg ccatattgct
    10081 gtacttgatc cctggccaca aacgacgggc cagtttcata gtgcaaatcc actcaccggg
    10141 catctcaggc agtacgcggc ggtcaaagct ggcgttgtgc gcgacatacc attcactacc
    10201 gtaatagtgt gggatcacat cttcaatcca cggtttatcg gcgaccatgg cttcggtgat
    10261 gcgatgaatc gccatcgctt gtggactaat aggacgatcg gggcgcacca ggtggctcat
    10321 ggggttgacg atttttccgt caatgacatc aacagaggca atctcaacga tccctccctg
    10381 caaaccgcag gtttctgtat cgataatgcg caacatggca tgctccaggc cgaaaaagcc
    10441 tagcgtaatg gaatgatatc gccttgccaa ccctgtgatg aacgctgacc gactaacaat
    10501 aatgaaccac gatcggcgcg aacgatgaga cgaccatgtt catcccatag tgcactgtta
    10561 ccacgggcat ttgccatcag cacggcaatt gagtactgat gtgaaaaaaa ttgtaagcgg
    10621 cgggtagatg ccagtaggtc gggttccccc agacattgcc ctgtcgtaaa cagtgaacag
    10681 gtcgggtcca tatccatacc ttccggttgt tcatcgacca ccgtgatggt tctggaacga
    10741 cggcccagac atgcaccatg actctgatgg tagatccctg gtgtttttcg ccacggtgcg
    10801 aacaccgcaa ttccacgaat aaagcgatcg ttatattcaa cgggaaggcc ggcaatgatg
    10861 gtcattcgcc aggtcgttgc agcataacaa agcgggtcta acagcgagag atcgggtggg
    10921 gcaggaaggg cacgtcgcga ataatcacac cccagtaaag aaagcgatgg aaaaactaat
    10981 agctgacatt gttgcctggc ggcggcactc acaaactcca gatgatgagc tacctgttcg
    11041 gtcagtgagg ttttacgagg ctcatattgc gctgctgcaa ctttccagaa cgacataatg
    11101 acttcctttt caaaatgact gcaaattatg ggtgcggttt agaaaatact cataatttag
    11161 caggcgttat gtaagaaagt gtaactctgt ataagtttta tttaagtttg ggctcgtaag
    11221 gtaaacgtga cagattgacc gaagccaaac ggtgggcgat aagccgctcg cgaaaccagc
    11281 tgcgcaaatg ttcaggctgt tcacgttcaa ccgcttcagc gatcaccggc atattgtagc
    11341 gttctttaaa tgccaccccg gccgccgcca aatcgacatt cactttatcc atttctgttt
    11401 gatccagttt agccagattc ttcagcattc ttaatctcct catcattcgc gggcaaaagt
    11461 tactaagagt gagcctggat ggcaagccgg aattgatgat cctcgactca gacattagac
    11521 aaggttatgc tctgtaaaaa agttattaca aaaaggaata gataatatgg cttcaactgc
    11581 acgctccctt cgctacgcgc tggcaatcct gaccacttcg ttggtgacac cttccgtctg
    11641 ggcacatgcg catttaacgc atcagtatcc tgcggcaaat gcgcaagtga cagctgcccc
    11701 gcaggcaatc actttaaact tctcggaagg cgttgaaaca ggattcagtg gtgcaaaaat
    11761 cacggggcca aaaaacgaaa atattaaaac attaccggcg aagcggaatg agcaggacca
    11821 aaagcaactg attgttccac tggccgattc gctgaaaccc ggaacctaca ccgttgactg
    11881 gcatgttgtt tcggtggacg ggcataaaac gaaaggacac tacaccttta gcgtgaaata
    11941 aatcatgctg gcgtttacct ggatcgcgct gcgatttatc catttcacct cgttgatgct
    12001 ggtttttggc ttcgcaatgt acggtgcctg gctggccccc ttaacgattc gtcgcttgct
    12061 gacgaagcgt tttctgcgtt tacaacagca cgctgccgtc tggagtttga tcagtgccac
    12121 tgcaatgctc gccgttcagg gcgggttgat gggaacggga tggtcagatg tattttcgcc
    12181 aaacatttgg ctggctgttt tgcaaacgca gtttggtggc atctggttat ggcaaattgt
    12241 tctcgccctc gtaacgttga tcgttgccct tatgcaaccg cagaatatgc cacgtttgct
    12301 atttatgctc actaccgcgc aatttattct gctggcggga gtcgggcatg cgacgctgaa
    12361 tgaaggggta acggcgaaaa tccatcagac taatcacgca attcacctga tctgtgcggc
    12421 ggcgtggttt gggggattac tgccggtgct ctggtgtatg caactcatca aaggccgttg
    12481 gcgacaccag gctattcagg cgctgatgcg tttttcctgg tgcgggcatt ttgcggtgat
    12541 cggcgtactg gcaagcggcg tgcttaatgc attgttgatt accggatttc cccccacgct
    12601 tacgacttac tggggccaac tcttaatgct caaagccatc ctggtgatga ttatggtggt
    12661 catcgcgctg gctaatcgtt atgttcttgt accgcgcatg aggcaggatg aagatcgtgc
    12721 ggctccctgg tttgtgtgga tgactaaact cgaatgggcg ataggtgcgg tagtgctggt
    12781 gatcatcagc ctgcttgcga ctctcgaacc tttttgatgg attacgaaaa tgatgaaaaa
    12841 aagtattctg gcgtttctgt tactcaccag ttctgcagcg gcgctggcgg caccgcaggt
    12901 gattaccgtc agccgttttg aagtgggtaa agacaaatgg gcgtttaatc gcgaagaggt
    12961 gatgctgact tgccgaccgg gtaatgcttt gtatgtcatc aacccaagta ccctcgtgca
    13021 gtatccttta aacgatatcg cacaaaagga agttgccagt gggaagacta aagcccaacc
    13081 catttcggtg attcagattg atgatcctaa caatcccggc gaaaaaatga gtctggcacc
    13141 gtttatagag cgagctgaaa aactctgtta attacctaaa atagcctttt gatttccaat
    13201 aaaaaaccgc ctcagttctt tcaccagaac gggcggtttt taacatttaa gctgatgacc
    13261 accacgcttt ttattgacca ttttgcacgc aaactggaaa acctggcgtc gtcatctatt
    13321 cttaaagagc aaggcaacta agcctgcatt aatgccaact tttagcgcac ggctctctcc
    13381 caagagccat ttccctggac cgaatacagg aatcgtgttc ggtctctttt tatctgttaa
    13441 aagccagaag cattcccttc gctgacttta tagtcaacca taacacacac tctactgtct
    13501 gcgtccagcg ttttttaaca ttcttgttaa gattatgtga tctttagcgc gggaggaaaa
    13561 tattgatgaa acagcctgcg cccgtttatc agagaattgc gggtcatcaa tggcgacata
    13621 tctggctttc aggcgatata cacggttgtc ttgagcagtt gcgccgcaaa ttatggcatt
    13681 gtcgttttga tccgtggcga gatttactta tctcagtggg agacgttatc gatcgtgggc
    13741 cgcaaagttt acgttgcctg cagttactgg aacaacattg ggttcgtgcg gtaagaggca
    13801 atcatgaaca gatggcgatg gatgcgctgg catcccagca gatgtctttg tggttcatga
    13861 atggcggcga ctggtttatt gcgctggcag ataatcaaca gaaacaagcg aaaacggcgc
    13921 tggaaaaatg tcagcatttg ccctttattc ttgaagtaca cagtcgcacc ggcaaacatg
    13981 ttattgccca tgccgattat ccggatgatg tttatgaatg gcaaaaggac gttgatttgc
    14041 atcaggtctt gtggagccgc tcgcgattag gtgaacgccc aaaagggcag ggaattaaag
    14101 gtgctgatca tttctggttt ggtcatacac cgttgcgaca tcgcgtggat attggcaacc
    14161 tgcattatat tgatatcggt gctgtctttg ggggcgaact gactcttgtg caattgcaat
    14221 aattaaaaat caccgtactc ctgtgctggt cgccagaaac catcaataaa atcctcgatt
    14281 ggaaaacaac cgccatggcg gatccgttga tcgctcatag aataaagaca ctgctgttcc
    14341 gtgttgtaga catccacaac aatatcttca caaccgccat ccaggtagca aacaaaaagt
    14401 accagcgcga acatttcatc cccgaagtgt ggtgccgtac cgttaagttt aggagagatt
    14461 ttacaacggg gaaataacca ggacaaataa cccgccatgc cgacgggttc tttttggatc
    14521 aggcaagacg cataatccag gcatcagatt gttgatcgta acgggtacgg taaaggacag
    14581 agtgttcgcc atttagcagg gcaggaatac tggtttcagc atcccaggca tccagttgca
    14641 tacataaatc ggggtctgat ttacaaggaa tggttaacgt tcgatcaccc ggcgattcgc
    14701 cgtgtaattc gccgtcgtcg atttcaaaag cgcctatgcg cacactggtt ttcatcttcg
    14761 tgctctccat ttagctgccg gttgtggtac gaccagtccg atcgcaccat ttttcagcct
    14821 agttgaggca gccgaatgtg ccagtcaaaa agtgcgcttt ttttgtgcgc tgtcaggcgt
    14881 taccggtaaa aagtttccca tcgcgcacca gttcacgcgg gtagctgttt ttcagtcgcg
    14941 aaccaatccg tttggctaaa ccaatcggct gatgctggaa agtaaccaat acatcatccg
    15001 ctactggcgc ggcttgcggg taaacatcgc gcccgcgata ccactcttcc gcttcctgcg
    15061 gtgtcagttc aaaagcgttc acattgtcgg gggaggcaag ggcaataacg gcttcatgct
    15121 gccagcgata acctttgttg tgcgtttcgg caagtttaat ccccaagcga gaaaatcgga
    15181 ctttaccgat cagggcttca atgcccaccg ggaacaacca cagttctttg tcacgctgcc
    15241 agagtcgcag gttttcatcc cagtttaagc caacacttgc agccgcctga cgaatttgtc
    15301 cggcttcgcg atctttcacc gggctgaacg ggaaattgcc cactttgtat tttggggtgg
    15361 gtaaggcggg aatcgcctga gttttacgca gacgagcaac gaagaagcct tcgcagtcgt
    15421 aaatttgtgg gaaaacatgc aaaaagcctt cctcggtcag cgccttgttt gcaccaggga
    15481 agagatcgcc aagcggtaaa aactctactg cgtcgggata agtctctttc agccacaggc
    15541 aaacggcttc gttttcttcc cggtttaagg tacaggtcga gtaaaccagc gtaccgccag
    15601 gacgtaatgc atgaaaggcg ctgtcgatca gttcccgttg ggtcgctgcg atttcctgat
    15661 tgctttctgg tgaccagttt tttagcgcat cgggatcttt acgcaccacg ccttcgccgg
    15721 agcagggagc gtccagcaaa atggcatcga acatttctgg cactgccgca ccaaatacgc
    15781 ggccatcaaa atgcgtgagc gcaacattac tgatgccaca gcggctgata ttggcatgta
    15841 acacttttac ccgactggcg gaaaactcat tggcaaggat tgccccttcg ttattcatcc
    15901 gcgcggcaat ttgcgtcgtt ttggagccgg gcgcggcagc gacatccatc acccgctgtg
    15961 gtgcattacc gtcagcaaac aaggcggcaa caggcaacat tgaactggct tcctgaatat
    16021 aaaacaggcc gcttaaatgc tcggcggtac tacccaacgg caatgcatct tcactgtcgc
    16081 gttcaatcca gaaaccttct tcacaccacg gaattggcgt aagcgtccag ccataaggag
    16141 cagttaattg caggaaatca gcaacggagg ttttcagcgt attaacgcga atgctgcggc
    16201 gcaacgggcg ctgacaggcg gcaagaaaat catcaaaaga gagcgtcgaa ggcatcgctt
    16261 cgcgcatttg tgtcagaaag gcgtccggga aataaacggt gtgttgggcc acgagcatat
    16321 accactggca aaaaaacagg cgcgcagttt agcataaacg ctccggcgca ggcacgccgg
    16381 agcagtgggc attatttggg aagcgcagtt ccccattcac gccactcttt cggttcactt
    16441 tcctgcaaca ggaagtgctt gccttcctgg gctttcggtg ccagtggcgt acccggaggc
    16501 gtggcgaagg cgatgccgcc acggataaac tggttaaagg tgccggtttt cactacgccg
    16561 cccgtcagac caaagtccag actgtaaccc gatgccaacc agaagacgga attgttacgc
    16621 accaggtgtt gatagcgttt actgatgcgc atcgcaatca tcacgcgatc tgacaatgtc
    16681 cccagcgtca ttccggtaac cgtaccgact tccagaccac ggaacagcac aggcgtaccg
    16741 atgcctaacg aaccggcttc cggcgcttca acaataatgc ttaagccatc caggtaacgc
    16801 gaatcagtta tggtagcctc ttgtaattca aagtcgcgcc gaggattgcc ccggcctggt
    16861 tcgacgttga tatacggctg gaggatagta tcaagatgct caacgccagc tgccgaaatt
    16921 tgcggtgtga ccactgagaa gcgtgtacca ccgcgtgcaa aggtctggac atattccggg
    16981 taaagtaccg cctttgcctg tacttcattg cgcgcggtaa tcagatccag cgtctggatt
    17041 tgcccgatat caataccgag atagcgaatt ggcatcccga ccgccagttt tccggcatcg
    17101 aaagcgtgaa gcgtaatttg cccgccaacc gcacgggccg ctgtttcgga agcatacaga
    17161 atacgcttgt cgcctttacg ctgactggcg ctggcaccgc tgaggttatc gaagctaata
    17221 gcccccttta atgctctgga gagcggggat gcctgtacgg tcagaccact accatttagc
    17281 tgaactttcg ccccaccttc tgcccagaac acgctgttgc tggtcagaag gttgcgatac
    17341 tccggcttaa tatgcagatc gatatcaaac gcgttagctc gcggacgcac agtaataact
    17401 tcaccaactt caaatttacg gtacagcact accgatcctg cctgcacatc cggcagcgtc
    17461 tctgcactca aacttacggt tgtggtgggt aaatcgctaa ggctgttctc cagcgctttt
    17521 tccagattgg catacagtgg atagctggct ttcatctcgc ctttatcgcc cggcagaata
    17581 cgtatcccgc cgttaatcca ttctgaggcg ctggcaccga gaaactcaac gccatccagc
    17641 cccaccttca cgtcgacacg gctgttgacg acaaatttgc tatcgccttt taccagttcg
    17701 cgatgctgag gctcgatggc gacggtaaag gtaacgcctt tgctggtgag tttacgatca
    17761 ataacctggc ctacttgcac accgtggaga atgagcggct gaccagcatc aataccgtaa
    17821 ctttctggtg ctgtcagggt cagtgtcaga acatccggct cttgtagcag ggctttttcg
    17881 cctggcacaa caacgaactc tttgcgtggc tcaccatcgc cgggcaccaa ctcaaaggtt
    17941 tttccggtca gcagggcgct gagattggca tcgctaaggg ataatttcgg attacgtaat
    18001 tcgatgcggg tattttcccg taacagcgta acgacgctgg gatcaacggt catttctccg
    18061 gtgactttac caccaggatt taaatccagt ttagtcagct gtccgacttc cagcccctga
    18121 tacattaacg gcgtcgagtc ggcggttaat ccggccccac tcggcagttc cagttttatt
    18181 attacgccac gctggctgtg ggcgagatct tcatacagac caaaggtatc ttccgcctcg
    18241 gcaggtttcg actcttcagg tgaatcgaag gcaatcgcac cgttaaccag tgccgccaga
    18301 ctttccagtt tcaccttcgc gccactgata ctgacgttgg catcaacgcc ggaaacgttc
    18361 cagaaacggc tacctttttt caccaggtcg gtaaaacgcc gctcgatcag gacatcaatc
    18421 accacgcctt gcttattggg attgatggca tagtcgtaga ctttacccac cgggatctta
    18481 cggaaataga ccaatgaacc gctgttcagt gaaccgagat cgggggcttg caggtggatc
    18541 atcagatcgc cattatccag ccgatacttc ggttgggtat cgagtgcgac aaagtgatcc
    18601 tgctctttac ctttacctgg catcatgccg atatagttac caccgacgag ggcatccagc
    18661 ccggagacac ctgccagtga ggcctttggc gtcaccagcc agaactgtgt ctcttcgcgc
    18721 agcgcatctt tcatatcaga cttgatgctg accttgactt caatcttacg aagatcgtcg
    18781 ctcaggctga tatcctgcac agttccgact tcaacgccct gataacgaac aggggtgcgg
    18841 cccgggacaa taccatccgc cgacataaag tcgatggtga cggtattacc ccggtcctga
    18901 taactatccc aaatcagcca accggcaatc attagcgcga tgaacggcag cagccagaaa
    18961 ggtgagatac ggcgtttatt tttaatctgc gcttcagtcg tcgaagcggg cgtttcctga
    19021 ctcatgtgca tcccaaagta agcggctgtc cagccattcc acagcaagaa tagtcaaaat
    19081 taccgctgcg ccgaaataaa acgcagccgg tcccatagta aaagcgagga tctgatcgcg
    19141 attaatcagc gacatggtta aagatatgac aaacaggtcg agcatcgacc agcgaccaat
    19201 ccaggtcacc atccgcagta acagaatgcg tgtgcgtaaa ccttgctggc atttaaaatg
    19261 aatactcaac agtaaagtga acatgacgat cactttagta aacggtacca gaatactggc
    19321 gataaacacg attcccgcaa cggcaatgtt gctacttgcc agcgacataa tcccggaaag
    19381 aatcgtatct tcctgccggc caccattcag ataaatgata gaaataggca acaggttggc
    19441 aggtaacaac aaaacgattg acgctaacag cgccgcccag catttttgca gactatgacg
    19501 gcgacgcagg cgtagcggga tatggcaacg cgggcagcga ccacgctgat caggatagcc
    19561 ggtaaaatgg cacccaagac agacacggag tttctcgtct ctacgcgtag tggggcgctg
    19621 tggataaaat cgctcccaca gttcttcgac attgagatgt gacaacgtca ccgtcgttaa
    19681 aatcaccaac gccacaaaag agaacaagcc gacacccgcc tggatatggg cataatcttg
    19741 tacctttata gaagcaacgc caatgccgac caggtagata tccagcatta cccactcttt
    19801 aagtcgctca agcatcagca gtaccgggcg tagattcatt cccagtcggt taccaaacca
    19861 taaataagct atggaggtca ccagaatgag gggggcacca ataacgcaaa aaaagaccat
    19921 tgatcccgtt atcgcatcgc cctgtttggt catttgccag atgccttgca tgacgttggc
    19981 gtcaatacga atgcctaaca gccagatatg caacagcggt tcgccccagg caaacggcat
    20041 cagcaacagc atggtgaagg ccattgccgc caggcgcgtt agcgaccagt cgcgcccgtc
    20101 acgaattttt gcctgacagc gcggacaata ggcactttga tgagaattta tctcgggcag
    20161 gctaaacagc atgtcacatt gcgggcaacg ttggtaatca ccacgcggca gttcctcacc
    20221 gatagccctc actgttatct tttttgtcgg cgtaatttgt ggtgtgttaa gagccatgac
    20281 gtgccaaatg taaaaagaat taacattaat cttaacccat gattggaatg atcttgagca
    20341 ttaacgcatt taatgcttat tttaataggc tggcagttcc tggtaagaca accaagtgat
    20401 tacataatga acaaaataga attttacgcg gatttaaatc gcgactttaa cgcgctgatg
    20461 gcaggagaaa ccagttttct ggcaacgctt gcgaacacca gtgcgttgtt atatgagcgt
    20521 ctcactgacg taaactgggc aggtttttat ttgcttgagg acgatacact ggtactcgga
    20581 ccatttcagg gcaaaattgc ctgtgtccgg atacccgtcg ggcgcggcgt gtgcggcact
    20641 gcggttgccc gcaatcaagt gcagcgtatc gaggatgttc atgtgtttga cgggcatatt
    20701 gcctgtgatg cggcgagtaa ttctgaaatt gttctgccgc tggtggtgaa aaatcagatt
    20761 attggtgttc tcgacatcga tagtaccgtc ttcggtcgct ttacagacga ggacgagcaa
    20821 ggcttacgtc agcttgtggc acagcttgaa aaagtgcttg caacgacgga ttacaaaaaa
    20881 ttctttgcga gcgtcgcagg ataatcaacg gataacgtag catttactga tagcgtcatt
    20941 ataatgacgc ctgttcatgc ctgcgcttgt tggctacgtc cgttgtaatc aggaaatttc
    21001 atggaaaatc aacctaagtt gaatagcagt aaagaagtaa tcgcgtttct ggccgaacgt
    21061 tttccccact gtttcagtgc ggaaggtgaa gcgcgtccgc tgaaaatcgg tatttttcag
    21121 gatttggtcg atcgtgttgc cggggaaatg aacctgagca aaacgcaatt gcgatccgct
    21181 ttacgtctct acacttcgag ctggcgttat ctttacggtg ttaaacccgg cgcaacgcgt
    21241 gtcgatcttg acggcaaccc atgcggtgag ctggacgagc aacatgtaga gcatgctcgc
    21301 aagcagcttg aagaagcgaa agcgcgtgtt caggcacagc gtgctgaaca gcaagcgaaa
    21361 aaacgcgaag ctgccgcagc tgctggtgag aaagaagacg caccgcgccg cgaacgcaag
    21421 ccacgtccga ccacgccacg ccgcaaagaa ggcgctgaac gtaaacctcg tgcgcaaaag
    21481 ccggtagaga aagcgccaaa aacagtaaaa gcacctcgcg aagaacagca caccccggtt
    21541 tctgacattt cagctctgac tgtcggacaa gccctgaagg tgaaagcggg tcaaaacgcg
    21601 atggatgcca ccgtattaga aatcaccaaa gacggcgtcc gcgtccagct gaattcgggt
    21661 atgtctttga ttgtgcgcgc agaacacctg gtgttctgaa acggaggccg ggccaggcat
    21721 gaacatgttt tttaggctta ccgcgttagc tggcctgctt gcaatagcag gccagacctt
    21781 cgctgtagaa gatatcacgc gtgctgatca aattccggta ttaaaggaag agacgcagca
    21841 tgcgacggta agtgagcgcg taacgtcgcg cttcacccgt tctcattatc gccagttcga
    21901 cctcgatcag acattttcgg ccaaaatctt tgaccgctac ctgaatctgc tcgattacag
    21961 ccacaacgtg ctgttggcaa gcgatgttga acagttcgcg aaaaagaaaa ccgagttagg
    22021 cgatgaactg cgttcaggca aactcgacgt tttctacgat ctctacaatc tggcgcaaaa
    22081 gcgtcgtttt gaacgttacc agtacgcttt gtcggtactg gaaaagccga tggatttcac
    22141 cggcaacgac acttataacc ttgaccgcag caaagcgccc tggccgaaaa acgaggctga
    22201 gttgaacgcg ctgtgggaca gtaaagtcaa attcgacgag ttaagcctga agctggcagg
    22261 aaaaacggat aaagaaattc gtgaaaccct gactcgccgc tacaaatttg ccattcgtcg
    22321 tctggcgcaa accaacagcg aagatgtttt ctcgctggca atgacggcgt ttgcgcgtga
    22381 aatcgacccg cataccaact atctttcccc gcgtaatacc gaacagttca acactgaaat
    22441 gagtttgtcg ctggaaggta ttggcgcagt gctgcaaatg gatgatgact acaccgttat
    22501 caattcgatg gtggcaggag gcccggcagc gaagagtaaa gctatcagcg ttggtgacaa
    22561 aattgtcggt gttggtcaaa caggcaagcc gatggttgac gtgattggct ggcgtcttga
    22621 tgatgtggtt gccttaatta aagggccgaa gggcagtaaa gttcgtctgg aaattttacc
    22681 tgctggtaaa gggaccaaga cccgtactgt aacgttgacc cgtgaacgta ttcgcctcga
    22741 agaccgcgcg gttaaaatgt cggtgaagac cgtcggtaaa gagaaagtcg gcgtgctgga
    22801 tattccgggc ttctatgtgg gtttgacaga cgatgtcaaa gtgcaactgc agaaactgga
    22861 aaaacagaat gtcagcagcg tcatcatcga cctgcgtagc aatggtggtg gggcgctgac
    22921 cgaagcggta tcgctctccg gtctgtttat tccatccggc ccgattgttc aggtccgtga
    22981 taacaacggt aaagttcgtg aagacagcga taccgacgga caggtcttct ataaaggccc
    23041 gctggtggta ctggttgacc gtttcagtgc ttcggcttca gaaatctttg ccgcggcaat
    23101 gcaggattac ggtcgtgcgc tggttgtggg tgaaccgacg tttggtaaag gcaccgttca
    23161 gcaataccgt tcattgaacc gtatctacga tcagatgtta cgtcctgaat ggccagcgct
    23221 gggatctgta cagtacacga tccagaaatt ctatcgcgtt aacggcggca gtacgcaacg
    23281 taaaggtgta acgccagaca tcatcatgcc gacgggtaat gaagaaacgg aaacgggtga
    23341 gaaattcgaa gataacgcgc tgccgtggga tagcatcgat gccgcgacct atgtgaaatc
    23401 aggcgattta acggcctttg gaccggagct gctgaaggaa cataatgcgc gtatcgcgaa
    23461 agatcctgag ttccagaaca tcatgaagga tatcgcgcgc ttcaacgcca tgaaggacaa
    23521 gcgcaatatc gtttctctga attacgctgt gcgtgagaaa gagaataatg aagatgatgc
    23581 gacgcgtctg gcgcgtttga acgaacgctt taaacgcgaa ggtaaaccgg agttgaagaa
    23641 actggatgat ctaccgaaag attaccagga gccggatcct tatctggatg agacggtgaa
    23701 tatcgcactc gatctggcga agcttgaaaa agccagaccc gcggaacaac ccgctcccgt
    23761 caagtaatat caatcaggca caagaaattg tgcctgattt tttaacagca gcaagatgcc
    23821 gtaaatcaga tgctacaaaa tgtaaagttg tgtctttctg gtgacttacg cactatccag
    23881 acttgaaaat agtcgcgtaa cccatacgat gtgggtatcg catattgcgt tttgttaaac
    23941 tgaggtaaaa agaaaattat gatgcgaatc gcgctcttcc tgctaacgaa cctggccgta
    24001 atggtcgttt tcgggctggt actgagcctg acagggatac agtcgagcag cgttcagggg
    24061 ctgatgatca tggccttgct gttcggtttt ggtggttcct tcgtttcgct tctgatgtcc
    24121 aaatggatgg cattacgatc tgttggcggg gaagtgatcg agcaaccgcg taacgaaagg
    24181 gaacgttggc tggtcaatac ggtagcaacc caggctcgtc aggcggggat cgctatgccg
    24241 caagtggcta tctaccatgc gccggacatc aatgcttttg caaccggtgc gcgccgtgat
    24301 gcctctctgg ttgctgtcag caccggtttg ctgcagaaca tgagcccgga tgaagccgag
    24361 gcggtaattg ctcacgaaat cagccacatc gccaatggtg atatggtcac catgacgctg
    24421 attcagggcg tggtgaacac cttcgttatc tttatttccc gtattctggc gcagcttgcc
    24481 gcgggtttta tgggcggaaa tcgtgatgaa ggtgaagaga gcaacggcaa cccgctgatc
    24541 tactttgcgg ttgcaacggt tctggaactg gtgtttggta ttctggcgag cattatcacc
    24601 atgtggttct cgcgtcatcg tgaattccac gccgatgcgg gttcagcaaa actggttggt
    24661 cgcgagaaaa tgattgctgc attgcagcgc ctgaaaacca gctatgaacc gcaagaagca
    24721 accagcatga tggctttctg cattaacggg aagtcgaaat cgctcagtga gttgttcatg
    24781 actcacccgc cactggataa acgtattgaa gctctgcgta cgggtgaata cctgaagtaa
    24841 tatgaagtaa atactaaaaa gcgcgtcctg atcgacgcgc tttttttatg ccctggatcg
    24901 tggctgagtg atacgtaaac cactgacaca ggctgcaatc accgccagaa tagccgcagc
    24961 tatcagcgag acgtgcgtac cattatcacc aaactgattt agcatcagcg ccaccagagc
    25021 cgcgccgcta ctctgaccca gaagacgagc cgtccctaac atgccactgg ctccaccgct
    25081 acgttcgcga ggagcggagg taataatggt gtggttattg ggtgactgga acaagccaaa
    25141 tccagcacca cataagataa tcggccagat aatattgata tccgcaggtg acgctggcag
    25201 cagaactagg gaaaaaagcc ccgcagccat gatgaataac cctaaagcac ccagcaatcc
    25261 tgcatgtacg cgttcaatca aatagcctgc cagtggagcc atcaccattg ttgctaacgg
    25321 ccacggtgtc agaagtaaac ctgtttcgac ttcactacgc ccgagcacgg tttgcaggta
    25381 aaagggaagg gaaaccattg ccagcatttg tgcgcagaaa gagcaaacag atgtgcaaat
    25441 agaaagtgaa aacagcggga tacgcagtaa atccaccggt agcagcggta cgggaagaga
    25501 aagctggcgg cgaataaaga aaataccaac gacaaccatt accaccagtt ccgcaccaat
    25561 caatgtcagc gattgcccct gagcgaaacc actcaatgca gtaataagca ggccgaaggt
    25621 taacgcgttc atcacggcgc tgggcaggtc gaaacggggt ttactggcgc gagaaccatt
    25681 gggtggcaga aaacgcatcg ccagaagcag ggcgataata cccaacggta cgttgattaa
    25741 aaataaccat ttccaggatg cgatggagag gattgctgca gcaattgtcg gcccggcagc
    25801 agaagaaacg gcaacaataa acgagtttat gcccatccct ctacccagaa aacgttgtgg
    25861 atagatcagg cggataagtg cggtattaac gctcatcaac gctgcaccgc cgaaaccttg
    25921 tatgacacgc gcaagggtga gcatttgcag cgaatcagaa agggcgcaga atagtgaaga
    25981 caacagaaaa acgaccagac cgcatttata aatacgtcga tagccaaaca tatcgcccag
    26041 aaacgaaaac gagagcaggg agatgacaat ggcgatttga taggcgttca ctacccagat
    26101 ggaactggct ggcgtggcat gaaggtccgt ggcgattgtt ggcaaggcga cgttggcgat
    26161 tgcgccgtca aggacagcca tcgaaatacc aatcacaatg gttaatatcg caccgtatcg
    26221 ctggggcaat ggcaggccgt cggcctgaac ttttggcata ggaattttat atctttggtg
    26281 aataatcaac aggggtatga ttctagcact gatattcacg gtggatgtcg cagaattgta
    26341 actaaccggc aaaagat
//
LOCUS       DAZUCC010000051        26135 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZUCC010000051 DAZUCC010000000
VERSION     DAZUCC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.051"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            262..1560
                     /gene="tig"
                     /locus_tag="ACHY09_001854"
     CDS             262..1560
                     /gene="tig"
                     /locus_tag="ACHY09_001854"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS3884834.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            1806..2429
                     /gene="clpP"
                     /locus_tag="ACHY09_001855"
     CDS             1806..2429
                     /gene="clpP"
                     /locus_tag="ACHY09_001855"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS3884835.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            2555..3829
                     /gene="clpX"
                     /locus_tag="ACHY09_001856"
     CDS             2555..3829
                     /gene="clpX"
                     /locus_tag="ACHY09_001856"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFS3884836.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            4017..6371
                     /gene="lon"
                     /locus_tag="ACHY09_001857"
     CDS             4017..6371
                     /gene="lon"
                     /locus_tag="ACHY09_001857"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFS3884837.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            6562..6852
                     /gene="hupB"
                     /locus_tag="ACHY09_001858"
     CDS             6562..6852
                     /gene="hupB"
                     /locus_tag="ACHY09_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFS3884838.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            7044..8915
                     /gene="ppiD"
                     /locus_tag="ACHY09_001859"
     CDS             7044..8915
                     /gene="ppiD"
                     /locus_tag="ACHY09_001859"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS3884839.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVVQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKK
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            9066..9437
                     /gene="ybaV"
                     /locus_tag="ACHY09_001860"
     CDS             9066..9437
                     /gene="ybaV"
                     /locus_tag="ACHY09_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS3884840.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            9531..9929
                     /gene="fadM"
                     /locus_tag="ACHY09_001861"
     CDS             9531..9929
                     /gene="fadM"
                     /locus_tag="ACHY09_001861"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFS3884841.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(9981..10676)
                     /gene="queC"
                     /locus_tag="ACHY09_001862"
     CDS             complement(9981..10676)
                     /gene="queC"
                     /locus_tag="ACHY09_001862"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFS3884842.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLHHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(10741..12441)
                     /gene="ybaE"
                     /locus_tag="ACHY09_001863"
     CDS             complement(10741..12441)
                     /gene="ybaE"
                     /locus_tag="ACHY09_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFS3884843.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLLLLQLPARDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            12541..13359
                     /gene="cof"
                     /locus_tag="ACHY09_001864"
     CDS             12541..13359
                     /gene="cof"
                     /locus_tag="ACHY09_001864"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFS3884844.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGNVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            13512..13970
                     /gene="decR"
                     /locus_tag="ACHY09_001865"
     CDS             13512..13970
                     /gene="decR"
                     /locus_tag="ACHY09_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFS3884845.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            14000..15772
                     /gene="mdlA"
                     /locus_tag="ACHY09_001866"
     CDS             14000..15772
                     /gene="mdlA"
                     /locus_tag="ACHY09_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFS3884846.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            15765..17546
                     /gene="mdlB"
                     /locus_tag="ACHY09_001867"
     CDS             15765..17546
                     /gene="mdlB"
                     /locus_tag="ACHY09_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFS3884847.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVIMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDANTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            17727..18065
                     /gene="glnK"
                     /locus_tag="ACHY09_001868"
     CDS             17727..18065
                     /gene="glnK"
                     /locus_tag="ACHY09_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS3884848.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            18095..19381
                     /gene="amtB"
                     /locus_tag="ACHY09_001869"
     CDS             18095..19381
                     /gene="amtB"
                     /locus_tag="ACHY09_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFS3884849.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(19430..20290)
                     /gene="tesB"
                     /locus_tag="ACHY09_001870"
     CDS             complement(19430..20290)
                     /gene="tesB"
                     /locus_tag="ACHY09_001870"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFS3884850.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            20508..21077
                     /gene="ybaY"
                     /locus_tag="ACHY09_001871"
     CDS             20508..21077
                     /gene="ybaY"
                     /locus_tag="ACHY09_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFS3884851.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQPAI
                     QQLNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFVL
                     PFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPVQ
                     ASGGATATVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(21108..21419)
                     /locus_tag="ACHY09_001872"
     CDS             complement(21108..21419)
                     /locus_tag="ACHY09_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFS3884852.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            21582..21678
                     /gene="ffs"
                     /locus_tag="ACHY09_001873"
     ncRNA           21582..21678
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHY09_001873"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            21798..22151
                     /gene="ybaA"
                     /locus_tag="ACHY09_001874"
     CDS             21798..22151
                     /gene="ybaA"
                     /locus_tag="ACHY09_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFS3884853.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(22193..23743)
                     /gene="pdeB"
                     /locus_tag="ACHY09_001875"
     CDS             complement(22193..23743)
                     /gene="pdeB"
                     /locus_tag="ACHY09_001875"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFS3884854.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWSSTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(23907..24377)
                     /gene="ylaC"
                     /locus_tag="ACHY09_001876"
     CDS             complement(23907..24377)
                     /gene="ylaC"
                     /locus_tag="ACHY09_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFS3884855.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(24493..25044)
                     /gene="maa"
                     /locus_tag="ACHY09_001877"
     CDS             complement(24493..25044)
                     /gene="maa"
                     /locus_tag="ACHY09_001877"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFS3884856.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(25217..25435)
                     /gene="hha"
                     /locus_tag="ACHY09_001878"
     CDS             complement(25217..25435)
                     /gene="hha"
                     /locus_tag="ACHY09_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFS3884857.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(25461..25835)
                     /gene="tomB"
                     /locus_tag="ACHY09_001879"
     CDS             complement(25461..25835)
                     /gene="tomB"
                     /locus_tag="ACHY09_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFS3884858.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         6496 a         6251 c         7073 g         6315 t
ORIGIN      
        1 tctggatttt gcgctttttg cccagcattc agacgaaaat tgcccgggaa ttgtgaaaaa
       61 atacgcgaca gcgcgcaata accgttctcg actcataaaa gtgatgccgc tataatgccg
      121 cgtcctattt gaatgctttc gggatgattc tggtaacagg gaatgtgatt gattataaga
      181 acatcccggt tccgcgaagc caacaacctg tgcttgcggg gtaagagttg accgagcact
      241 gtgatttttt gaggtaacaa gatgcaagtt tcagttgaaa ccactcaagg ccttggccgc
      301 cgtgtaacga ttactatcgc tgctgacagc atcgagaccg ctgttaaaag cgagctggtc
      361 aacgttgcga aaaaagtacg tattgacggc ttccgcaaag gcaaagtgcc aatgaatatc
      421 gttgctcagc gttatggtgc gtctgtacgc caggacgttc tgggtgacct gatgagccgt
      481 aacttcattg acgccatcat taaagaaaaa atcaatccgg ctggcgcacc gacttatgtt
      541 ccgggcgaat acaagctggg tgaagacttc acctactctg tagagtttga agtttatccg
      601 gaagttgaac tgcagggtct ggaagcgatc gaagttgaaa aaccgatcgt tgaagtgacc
      661 gacgctgacg ttgacggcat gctggatact ctgcgtaaac agcaggcgac ctggaaagaa
      721 aaagacggcg ctgttgaagc agaagaccgc gtaaccatcg acttcaccgg ttctgtagac
      781 ggcgaagagt tcgaaggcgg taaagcgtct gatttcgtac tggcgatggg ccaggggcgt
      841 atgattccgg gctttgaaga cggtatcaaa ggccacaaag ctggcgaaga gttcaccatc
      901 gacgtgacct tcccggaaga ataccacgca gaaaacctga aaggtaaagc agcgaaattc
      961 gctatcaacc tgaagaaagt tgaagagcgt gaactgccgg aactgactgc agaattcatc
     1021 aaacgtttcg gcgttgaaga tggttccgta gaaggtctgc gcgctgaagt gcgtaaaaac
     1081 atggagcgcg agctgaagag cgccatccgt aaccgcgtta agtctcaggc gatcgaaggt
     1141 ctggtaaaag ctaacgacat cgacgtaccg gctgcgctga tcgacagcga aatcgacgtt
     1201 ctgcgtcgcc aggctgcaca gcgtttcggt ggcaacgaaa aacaagctct ggaactgccg
     1261 cgtgaactgt ttgaagaaca ggctaaacgt cgcgtagttg ttggcctgct gctgggcgaa
     1321 gttatccgca ccaacgagct gaaagctgac gaagagcgcg tgaaaggcct gatcgaagag
     1381 atggcttctg cgtacgaaga tccgaaagaa gttatcgagt tctacagcaa aaacaaagaa
     1441 ctgatggaca acatgcgcaa tgttgctctg gaagaacagg ctgttgaagc tgtactggcg
     1501 aaagcgaaag tgactgaaaa agaaaccact ttcaacgagc tgatgaacca gcaggcgtaa
     1561 tttacgcagc ataacgcgct aaattcgcac aaaggcccgt caccgccagg tggtgggctt
     1621 ttttttgtca tgaattttgc atggaaccgt gcgaaaagcc tctttcggtg ttagcgtaac
     1681 aacaaaagat tgttatgctt gaaatatggt gatgccgtac ccataacaca gggactagct
     1741 gataatccgt ccataaggtt acaatcggta cagcaggttt tttcaatttt atccaggaga
     1801 cggaaatgtc atacagcggc gaacgagata actttgcacc ccatatggcg ctggtgccga
     1861 tggtcattga acagacctca cgtggtgagc gctcttttga tatctattct cgtctactta
     1921 aggaacgcgt catttttctg actggccagg ttgaagacca catggctaac ctgattgtgg
     1981 cgcagatgct gttcctggaa gcagaaaacc cagaaaaaga tatctatctg tacattaact
     2041 ccccaggcgg ggtgatcact gccgggatgt ctatctatga caccatgcag tttatcaagc
     2101 ctgatgtcag caccatctgt atgggccagg cggcttcgat gggcgctttc ttgctgactg
     2161 caggggcaaa aggtaaacgt ttctgcctgc cgaattcgcg cgtgatgatt caccagccgt
     2221 tgggcggcta ccagggccag gcgaccgata tcgaaattca tgcccgtgaa attctgaaag
     2281 ttaaagggcg catgaatgaa cttatggcgc ttcatacggg tcaatcatta gaacagattg
     2341 aacgtgatac cgagcgcgat cgcttccttt ccgcccctga agcggtggaa tacggtctgg
     2401 tcgattcgat tctgacccat cgtaattgat gccagaggcg caactgtgcc gctatactta
     2461 tccagggcgg cacaacgctg taagcggctt gcgcctgaga atggcatttg cgtcgtcgtg
     2521 tgcggcacaa agaacaaaga agaggttttg acccatgaca gataaacgca aagatggctc
     2581 aggcaaattg ctgtattgct ctttttgcgg caaaagccag catgaagtgc gcaagctgat
     2641 tgccggtcca tccgtgtata tctgcgacga atgtgttgat ttatgtaacg acatcattcg
     2701 cgaagagatt aaagaagttg caccgcatcg tgaacgcagt gcgctaccga cgccgcatga
     2761 aattcgcaac cacctggacg attacgttat cggccaggaa caggcgaaaa aagtgctggc
     2821 ggtcgcggta tacaaccact acaaacgtct gcgcaacggc gataccagca atggtgtcga
     2881 gttgggcaaa agtaacattc tgctgatcgg tccgaccggt tccggtaaaa cgctgctggc
     2941 tgaaacgctg gcgcgcctgc tggacgtccc gttcaccatg gccgacgcaa ccacgctgac
     3001 cgaagccggt tatgtgggcg aagacgttga aaacatcatt cagaagctgt tgcagaagtg
     3061 cgattacgac gtacagaaag cgcagcgcgg gattgtctac atcgatgaaa tcgacaagat
     3121 ttctcgtaag tcagacaacc cgtccattac ccgagacgtt tccggtgaag gcgtacagca
     3181 ggcactgttg aaactgatcg aaggtacggt agctgctgtt ccaccgcaag gtgggcgtaa
     3241 acatccgcag caggaattct tgcaggttga tacctctaag atcctgttta tttgtggcgg
     3301 tgcgtttgcc ggtctggata aagtgatttc ccatcgtgta gaaaccggct ccggcattgg
     3361 ttttggcgcg acggtaaaag cgaagtccga caaagcaagc gaaggcgagc tgctggcgca
     3421 ggttgaaccg gaagatctga tcaagtttgg tcttatccct gagtttattg gtcgtctgcc
     3481 ggttgtcgca acgttgaatg aactgagcga agaagctctg attcagatcc tcaaagagcc
     3541 gaaaaacgcc ctgaccaagc agtatcaggc gctgtttaat ctggaaggcg tggatctgga
     3601 attccgtgac gaggcgctgg atgctatcgc taagaaagcg atggcgcgta aaaccggtgc
     3661 ccgtggcctg cgttccatcg tagaagccgc actgctcgat accatgtacg atctgccgtc
     3721 catggaagac gtcgaaaaag tggttatcga cgaatcggta attgatggtc aaagcaagcc
     3781 gttgctgatt tatggcaagc cggaagcgca acaggcatct ggtgaataat taaccattcc
     3841 catacaatta gttaaccaaa aaggggggat tttatctccc ctttaatttt tcctctattc
     3901 tcggcgttga atgtggggga aacatcccca tatactgacg tacatgttaa tagatggcgt
     3961 gaagcacagt cgtgtcatct gattacctgg cggaaattaa actaagagag agctctatga
     4021 atcctgagcg ttctgaacgc attgaaatcc ccgtattgcc gctgcgcgat gtggtggttt
     4081 atccgcacat ggtcatcccc ttatttgtcg ggcgggaaaa atctatccgt tgtctggaag
     4141 cggcgatgga ccatgataaa aaaattatgc tggtcgcgca gaaagaagct tcaacggatg
     4201 agccgggtgt aaacgatctt ttcaccgtcg ggaccgtggc ctctatattg cagatgctga
     4261 aactgcctga cggcaccgtc aaagtgctgg tcgaggggtt acagcgcgcg cgtatttctg
     4321 cgctctctga caatggcgaa cacttttctg cgaaggcgga gtatctggag tcgccgacca
     4381 ttgatgagcg ggaacaggaa gtgctggtgc gtactgcaat cagccagttc gaaggctaca
     4441 tcaagctgaa caaaaaaatc ccaccagaag tgctgacgtc gctgaatagc atcgacgacc
     4501 cggcgcgtct ggcggatacc attgctgcac atatgccgct gaaactggct gacaaacagt
     4561 ccgttctgga gatgtccgac gttaacgaac gtctggaata tctgatggca atgatggaat
     4621 cggaaatcga tctgctgcag gttgagaaac gcattcgcaa ccgcgttaaa aagcagatgg
     4681 agaaatccca gcgtgagtac tatctgaacg agcaaatgaa agctattcag aaagaactcg
     4741 gtgaaatgga cgacgcgccg gacgaaaacg aagccctgaa gcgcaaaatt gacgcggcga
     4801 aaatgccgaa agaggcaaaa gagaaagcgg aagcagagtt gcagaagctg aaaatgatgt
     4861 ctccgatgtc ggcagaagcg accgtagtgc gtggttatat cgactggatg gtacaggtgc
     4921 cgtggaatgc gcgcagcaag gtcaaaaaag acctgcgtca ggcgcaggaa atccttgata
     4981 ccgaccatta tggtctggag cgcgtgaaag atcgcatcct tgagtatctt gcggttcaaa
     5041 gccgtgtcaa caaaatcaag ggaccgatcc tctgcctggt agggccgccg ggggtaggta
     5101 aaacctctct tggtcagtcc attgccaaag ccaccgggcg taaatatgtc cgtatggcgc
     5161 tgggcggcgt gcgtgatgaa gcggaaatcc gtggtcaccg ccgtacttac atcggttcta
     5221 tgccgggtaa actgatccag aaaatggcga aagtgggcgt gaaaaacccg ctgttcctgc
     5281 tcgatgagat cgacaaaatg tcttctgaca tgcgtggcga tccggcctcc gcactgcttg
     5341 aagtgctgga tccagagcag aacgtagcgt tcagcgacca ctacctggaa gtggattacg
     5401 accttagcga cgtgatgttt gtcgcgacgt cgaactccat gaacattccg gcaccgctgc
     5461 tggatcgtat ggaagtgatt cgcctctccg gttataccga agatgaaaaa ctgaacatcg
     5521 ccaaacgtca cctgctgccg aagcagattg aacgtaatgc actgaaaaaa ggtgagctga
     5581 ccgtcgacga tagcgccatt atcggcatta ttcgttacta cacccgtgag gcgggcgtgc
     5641 gtggtctgga gcgtgaaatc tccaaactgt gtcgcaaagc ggttaagcag ttactgctcg
     5701 ataagtcatt aaaacatatc gaaattaacg gcgataacct gcatgactat ctcggtgttc
     5761 agcgcttcga ctatggtcgc gcggataacg aaaaccgtgt cggtcaggta actggtctgg
     5821 cgtggacgga agtgggcggt gacttgctga ccattgaaac cgcgtgcgtt ccgggtaaag
     5881 gcaaactgac ctataccgga tcgcttggcg aagtgatgca ggagtccatt caggcggcgt
     5941 taacggtggt tcgtgcgcgt gcggaaaaac tggggatcaa ccctgatttt tatgaaaaac
     6001 gcgacatcca cgtccacgta ccggaaggtg cgacgccgaa agatggtccg agtgccggta
     6061 ttgctatgtg caccgcgctg gtttcttgcc tgaccggtaa cccggttcgt gctgatgtgg
     6121 caatgaccgg tgagatcact ctgcgtggtc aggtactgcc tatcggtggt ttgaaagaaa
     6181 aactactggc agcgcatcgc ggcgggatca aaacagtgtt aattccgttc gaaaataaac
     6241 gcgatctgga agagattcct gacaacgtaa ttgccgatct ggatattcat cctgtgaaac
     6301 gcattgagga agttctgact ctggcgctgc aaaatgaacc gtctggcatg caggttgtga
     6361 ctgcaaaata gtgacctcgc gcaaaatgca ctaataaaaa cagggctggc aggctaattc
     6421 ggacttgcca gccttttttt gtctcgctaa gttagatggc ggattgggct tgcccttatt
     6481 aaggggtgtt gtaaggggat ggctggcctg atataactgc tgcgcgttcg taccttgaag
     6541 gattcaagtg cgatataaat tataaagagg aagagaagag tgaataaatc tcaattgatc
     6601 gacaagattg ctgcaggggc tgatatctct aaagctgcgg ctggccgtgc gttagatgct
     6661 attattgctt ccgtaactga atctctgaaa gaaggggatg atgtagcact ggtaggtttt
     6721 ggtacttttg ccgttaaaga gcgtgctgcc cgtactggcc gcaatccgca gaccggtaaa
     6781 gagatcacca tcgctgctgc taaagtaccg agcttccgtg caggtaaagc actgaaagac
     6841 gcggtaaact aagcgttgtc cccagtgggg atgtgacgaa gttcaagggc gcatctactg
     6901 atgtgccttt tttatttgta ttcggtgact ttctgcgtct tgtgggctga caattgcccc
     6961 cgtttcttgt cacaatatgc ctttgcgcgc atcgatacgt tgcgtgaggt acacagtcat
     7021 ctacagcgga gtgttgttac accatgatgg acagcttacg cacggctgca aacagtctcg
     7081 tgctcaagat tattttcggt atcattatcg tgtcgttcat attgaccggc gtgagtggtt
     7141 acctgattgg cggaggcaat aactacgccg caaaagtgaa tgaccaggaa atcagtcgtg
     7201 ggcaattcga gaacgccttc aacagcgagc gtaatcgcat gcagcaacag ctgggcgatc
     7261 aatactccga gctggcagcg aacgaaggct atatgaaaac cctgcgtcaa caggtgctga
     7321 atcgtctgat cgacgaggcg ctgctggatc agtacgctcg tgagctgaaa ctgggtatca
     7381 gcgatgagca ggttaaacag gcgattttcg cgaccccagc cttccaggtt gacggcaaat
     7441 ttgataacag ccgctataac ggtatcctca accagatggg gatgaccgcc gatcagtacg
     7501 cccaggcgct gcgtaaccag ctcactaccc aacagctgat taacggcgtt gccggtaccg
     7561 attttatgct gaaaggtgaa accgacgagc tggcggcact ggtagttcaa caacgcgtgg
     7621 tgcgtgaagc gactatcgat gttaacgcgc tggcggcgaa gcagcctgtg accgaacagg
     7681 aaattgccag ctactacgaa caaaacaaaa acaatttcat gacgccggaa caattccgcg
     7741 tgagttacat caagctggat gccgcaacga tgcagcaacc ggttagcgat gcggatatcc
     7801 agagctacta cgatcagcat caggatcaat tcacccagcc gcagcgtacc cgttacagca
     7861 tcatccagac caaaactgaa gatgaagcga aagcggtact tgatgagctg aataagggcg
     7921 gtgattttgc tgcattagcc aaagaaaaat ctgccgatat tatctctgcc cgcaacggcg
     7981 gtgatatggg ttggttagaa gatgccacta tcccggacga actgaaaaat gctggtctga
     8041 aaaaaaaagg ccaactgtct ggtgttatca aatcttcggt cggtttcctg attgtgcgtc
     8101 tggacgacat tcagccagcg aaagtgaaat cgttagacga agtacgtgac gacattgcgg
     8161 cgaaagtgaa acacgaaaaa gccctcgatg cgtactacgc gctgcagcag aaagtgagcg
     8221 atgcggcaag caacgacacc gagtctctgg ccggtgcaga gcaagctgcc ggcgttaaag
     8281 ccactcagac gggttggttc agcaaagata acctgccgga agagttgaac ttcaagccag
     8341 ttgccgacgc tatcttcaac ggcggtctgg tcggtgaaaa cggcgcgccg ggcatcaact
     8401 ctgacatcat caccgtagac ggcgaccgcg cattcgtgct gcgcatcagc gagcacaaac
     8461 cggaagcggt gaaaccgttg gcagatgttc aggaacaagt taaggcactg gttcagcaca
     8521 acaaagctga acaacaggcg aaagtggatg ctgagaaact gctggttgat ttgaaagcag
     8581 gcaaaggtgc ggaagctatg caggctgccg gtctgaaatt tggcgagccg aaaaccttaa
     8641 gtcgttccgg tcgtgacccg attagccagg ctgcgtttgc actgccgctg ccagcgaaag
     8701 acaaaccgag ctacggtatg gcgaccgata tgcaaggtaa tgtagttctg ctggcgctgg
     8761 atgaagtgaa gcaaggttca atgccggaag atcagaaaaa agcgatggtg cagggtatca
     8821 cccagaacaa cgcacaaatc gtctttgaag ctctgatgag taacctgcgt aaagaggcga
     8881 aaatcaaaat tggcgatgcg ctggaacagc aataatcctg aagccgcctc gcaaaaaaat
     8941 gctttgcagc tgtaacaaga aaaggtcgct ttcgcggcct tttccatttc tgaacattgc
     9001 catttgttta ctgttttcac tgccgttaag gtgattccac tgttaacaaa caaggagaaa
     9061 acagtatgaa acacggaatt aaagcactgc tcattaccct gtccctggcc tgtgccggaa
     9121 tgtctcatag tgctctggcg gcagctcctg cggcaaaacc gacaacggta gaaaccaaag
     9181 cggaagctcc tgcagcacaa agtaaagcag cagttccggc gaaagccagt gacgaagaag
     9241 gcacccgggt cagcattaat aatgccagcg cggaagagtt agcccgcgcg atgaatggcg
     9301 ttggcctgaa gaaagcgcag gcgattgtca gttatcgcga agagtacggt ccgtttaaaa
     9361 cggtggagga tctaaagcag gtgccgggga tgggcaattc gctggtggag cgtaatctgg
     9421 cggtattaac cctgtaatta atttgcatag tggcaatttt ttgccagact gaagaggtca
     9481 taccagttat gacctctgta cttataacaa caacgtaagg ttctcgcgct atgcaaacac
     9541 aaatcaaagt tcgtggatat catctcgatg tttaccagca cgttaataac gcccgctatc
     9601 tggagtttct cgaagaagcc cgctgggatg ggttggaaaa tagcgacagt tttcagtgga
     9661 tgacggccca taacatcgct ttcgtggtgg tgaatatcaa tattaactat cgtcgcccgg
     9721 cggtattaag cgacttgtta accattacga gccagttaca gcaattaaac ggtaaaagcg
     9781 gcatcttaag ccaggtcatt acactggagc cggaagggca ggtggtagcg gatgcgctta
     9841 ttacgtttgt ttgtattgat cttaaaacgc agaaagcatt agctctggaa ggggaattgc
     9901 gcgaaaagct cgagcagatg gttaagtaaa cgttttgtgg tgccggatgc tcaagccgca
     9961 tccggcgaca cccggaataa ttacttcaac ccggttttct gcttcatcgc tgccatcacc
    10021 gtcggtttat cggccagata atgatgcaaa ccgttggcac gtaaattaca cgccgcacaa
    10081 tgaccgcaac cgtcgccttt aatgccgtta taacaggtca gcgtttcgtt acggactaaa
    10141 tccagcttgc cgtaataatc tgccagcgcc caggtttccg ctttatcaat ccacatcagc
    10201 ggcgtttcaa aacgaatgtc tttcgccata cccagactga cggcatggtt cagtgctttc
    10261 acaaactcat cgcggcaatc cgggtagccg gagaaatccg tttcgcagac gccggtaatc
    10321 accgcttctg cttttacctg atacgcatat attgccgcca gtgtcaggaa caaaatatta
    10381 cgcccaggga caaacgtatt cgggatgcca tcggcttcag gttcataatc tggcactgga
    10441 atgctgtcac gtgtcaggct gctgaccgcc agctcgttga gcagcgttac atcaagaacc
    10501 ttatgcgcgc gtgcccccag tttcagcgcc agttcgcgtg ccacgtcgat ttccgcgcga
    10561 tgtcgctgac cgtaatcgaa cgtcacgcaa tggacttcat catattgttg taatgcctgc
    10621 accagacagg tggtagaatc ctggcctcca ctgaacacaa cgacagcacg tttcatagat
    10681 aatcctgctt gaacaataaa agcgttatgg taacgcctgc gatgaacccg gaccagctat
    10741 tcattgcgac ggcgcgggaa gccaggcttc ggtaaattca aaccagcctc gcggtgtcag
    10801 tcgcacaccg ttcacgccgg gaggggcgct aatgcgatag tgatagttga acagcggcgt
    10861 cagcgtcgca tctgccatta actggctaaa aaccgctttc agggcattaa atcggttttc
    10921 ctcatcaggc attacttgca ccgcgtccag tgtcgattgc aaatgtgcat atgctggagc
    10981 gtcgaaaaca tgtggccata gtggatcgca acgcagccat tgctccaggg tatattccgg
    11041 tgcttcgcca attaatctgt cgcccatcat gaggtctgcg tgtgccagta gggtcgtgtc
    11101 gtcccagttt tttgcgttat gaaaaataat tgtgagttca cagccttccg ctgccagtgt
    11161 cgcctgtaga cgttctgcca tggtatgaag ttctatcggt aggtgataaa ccagcgtcaa
    11221 ggttttcggt agtttgacct catccggtac ttgccattgc ggaatagtcc agcctggcag
    11281 taatgcatga ctggcggtga tcaggttttc tccgacttct aacgtttgta ataaaccgga
    11341 ttgatgaata atggaaatca cttttcgcgc ctgccagagg gacagtcggg gacttttgcg
    11401 caacgttaaa tagcaaaaac ctaaactgat gccactactg acctggctga cccgttgcag
    11461 ctcctccggt ttgccgatgg tgatttgcac gggatgccga caactggttc ccaaatcttt
    11521 ttcgaaaagc ggcggagtta tccagtactc aaccgcttta agcagcggat gacgtaaatg
    11581 gtaataatca tggctttcca ggcgcaccag ctccgctgtg aattgtgtta agcgaaaagg
    11641 acccgtgccg atcagtggga attgcggatg cgccagatgg ctgcaatagc tcgccagtcg
    11701 gtgcgcaagc cagtaatcgg ggcgatgtaa aaagaaggtc agacactgcg gatgggtgac
    11761 ttcaatacgc ttcacgctaa taaataattg atcccgtgct ggcagttgta acagcagcaa
    11821 taatcgctgg tgtaagtgtg aggcttttac tgcatcgccg ttatgccagt gtagggttga
    11881 acgaagataa aagtcccagc gtaacccgtc agtagaggtt tcccagtgat gtgctaaatc
    11941 gccaatcggg cgctgagtat tattatcgaa gcgggtcagg ccggaaaata tctgcccggc
    12001 cagatgctgc tcggcacggc cgggcaaaaa gcccggttgt agcggttcga gcgggcgata
    12061 gtagggaata cgcaacgtgg gcgtatcgtt ttgccattgt ccgcccataa acggctgtaa
    12121 cagagtgcgc agctcacctg gggccagttg cgccagctcc agcacatctt gctgctttcc
    12181 ggtttccagc gcctgttcca tcatcgcatt gcgtagcgat tctggcgtga ccagaaagcg
    12241 tagttgcccg cgttttccgc gtcctgactg cgcctgccac tccagccatc ccgcctcctg
    12301 tgcctggcgt aacagcgtac gaacatggcg ttcgctgcaa aaacagcgtt cggccagttc
    12361 gctgacggtg acggtttgcg gctttccggc ggaaggttgc cacagacgtt gatactggtt
    12421 aagacggttg agcagtcgca tataaacccg gaacaatatt atttaactat tcactattac
    12481 ttccgtatat atcaggtgat actcaatcac cattaaccgt gtcacagagt ggagaaagaa
    12541 atggctcgtc tggcagcatt tgatatggat ggcactttat tgatgcctga ccatcattta
    12601 ggtgagaaaa ccctctctac tttggcgcga ctgcgtgaac gcgacattac cctcactttt
    12661 gccactgggc gtcatgcgct ggagatgcag catattctcg gggcgctatc gctggatgca
    12721 tatttgatta ccggcaacgg aacgcgcgtg cattctctgg aaggtgaact tttacatcgt
    12781 gatgatttac ctgcggatgt cgcggagctg gtgctgtatc agcagtggga tacccgagcc
    12841 agcatgcata tcttcaatga cgacggttgg tttaccggga aagagatccc tgcgttgttg
    12901 caggcatttg tctatagtgg ttttcgttat cagataatcg atgtcaaaaa aatgccactc
    12961 ggcagcgtca ccaagatctg cttctgtggc gatcacgacg atcttacacg cttgcagatc
    13021 cagctatacg aagcattagg cgagcgtgca catttgtgtt tttccgccac ggattgcctc
    13081 gaagtgctgc cggtgggctg caataaaggc gctgcattga cggtgctgac ccaacattta
    13141 ggtttatcgt tgcgcgattg catggccttt ggtgatgcga tgaacgatcg cgaaatgtta
    13201 ggcaacgtcg gtagcggatt tattatgggc aatgcgatgc cgcaattgcg cgcggagctc
    13261 ccgcatttac cggtgattgg acattgccga aatcaggctg tctctcacta tttgacgcac
    13321 tggctggact atccacatct accttattcc cccgaataac gagatccctt ccagcaccgg
    13381 gcaattgccc ggtttttttt gcgttgaatt tgtcattttg tgccgtggtg tttaaaccgc
    13441 acagaataaa ttgtcgcgat ttcaccttta aaatagaatt aaaagagaaa aaaattctct
    13501 gtggaagggc tatgttagat aaaattgacc gtaagctgct ggccttacta cagcaggatt
    13561 gtaccctctc tttgcaggca ctggctgaag ccgttaatct gacaaccacc ccgtgctgga
    13621 agcgtctgaa acggctggag gacgacggta ttcttatcgg caaagtcgcc ctgctggatc
    13681 cggaaaaaat aggcctcggc ctgaccgcct ttgtgctgat aaaaacgcaa catcacagca
    13741 gcgaatggta ttgccgcttt gtcacggtgg ttaccgaaat gccagaagtg ctggggttct
    13801 ggcgcatggc cggtgaatac gattatctga tgcgcgtcca ggttgccgac atgaaacgct
    13861 acgacgagtt ttataagcgt ttggtaaaca gcgtgccggg gctgtcggac gtcacttcca
    13921 gcttcgcgat ggaacagatt aaatacacca cttctttacc catcgaataa atatccagaa
    13981 tcaggtcagg acacaacgcg tgcgattatt tgctcaatta agctggtatt tccgtcggga
    14041 atggcgtcgc tatctcgggg ctgtcgcctt gcttgtcatt atcgcgatgc tgcaactggt
    14101 tccgccaaaa gtggttggta ttgttgttga tggcgtgaca gaacaacact ttactaccgg
    14161 gcagatcctg atgtggatcg ccaccatggt gctgattgcc gttgtggttt atctcctgcg
    14221 ttacgtctgg cgtgtattgc tgtttggcgc gtcttatcaa ctggctgttg aactgcgtga
    14281 agattattac cgtcagctaa gccggcagca tcctgagttt tacctgcgtc atcgcacggg
    14341 tgacctcatg gctcgtgcga ccaatgacgt cgatcgcgtc gtgtttgccg ccggagaagg
    14401 ggtgctgacg ctggtggatt cactggtaat gggctgcgct gtgttgatta tgatgtctac
    14461 gcaaattagc tggcagttga ccttatttgc cctgttgccg atgccagtga tggcgatcat
    14521 gattaagcgc aacggcgatg ccttgcatga acgctttaag ctggcgcagg cggcgttttc
    14581 cagtcttaat gaccgcaccc aggaaagcct caccagtatc cgcatgatca aagcttttgg
    14641 tctggaagat cgccagtcgg cgttatttgc cgaggatgcc gaagataccg gcaaaaaaaa
    14701 catgcgggtg gcgcgtattg atgctcgttt cgacccgacc atctatatcg cgattggtat
    14761 ggcgaacttg ctggcgattg gcggcggtag ttggatggtg gtgcagggca gtttaacgct
    14821 gggccagctc accagtttta tgatgtattt aggtctgatg atttggccaa tgctggcgct
    14881 ggcatggatg tttaacatag tggaacgtgg tagtgctgcg tacagccgca ttcgcgcgat
    14941 gctggcggaa gcgccggtgg tgaatgatgg tagcgaaccc gtgccggaag gtcgtggcga
    15001 actggatgta aatattcgcc agttcacgta tccgcagact gaccatcctg cgctggaaaa
    15061 cgtcaatttc gccctgaaac ccggtcagat gctgggtatt tgcgggccga ctggttccgg
    15121 caaaagtacc ctgttgtcgc tcattcagcg tcatttcgac gtcagcgagg gggatatccg
    15181 ctttcatgat attcctctga cgaagttaca actcgatagc tggcgtagcc gcctggcggt
    15241 agttagccag acaccgttcc ttttttccga caccgtagcg aacaacatcg cgttgggttg
    15301 cccgaatgcc acccagcaag agattgagca tgtcgcgcgg ttagccagcg tgcatgacga
    15361 tattttgcgt ttaccgcaag gttacgatac agaagtgggc gagcgcggcg tgatgctttc
    15421 cggtgggcaa aaacagcgta tctccattgc tcgtgcgtta ttagtcaacg cggaaatcct
    15481 catccttgat gatgcgcttt cggcagtaga cggacgcaca gagcaccaga tcctgcataa
    15541 cctgcgtcag tgggggcagg gaagaacggt aatcatcagc gcccatcgcc tttctgcact
    15601 gacggaagcc agtgaaatta ttgtgatgca gcacggacat atcgctcagc gtggcaatca
    15661 tgatgagctg gcgcaacaaa gcggctggta tcgcgatatg tatcgctatc aacaactgga
    15721 ggcggcgctc gacgacgctc cggaaattcg cgaggaggcc atcgatgcgt agttttagcc
    15781 aactgtggcc gactctcaag cgtctgttag cgtacggttc gccgtggcgt aaaccgctgg
    15841 ggattgcggt cataatgatg tgggttgcgg cagcggcaga agtcagtggg ccgctgctta
    15901 tcagctattt tatcgacaat atggtagcga aaaataacct gccgttgaaa gtggttgcag
    15961 ggctggctgc ggcgtatgtt gggctgcaac tgtttgccgc cgggctacat tacgcgcagt
    16021 cgctgctgtt taatcgggcg gcagtaggcg tagtgcaaca gttgcgtacc gacgtgatgg
    16081 atgctgcgtt acgccagcca ttaagcgagt ttgataccca acccgtcggg caggtgattt
    16141 cccgcgtcac taatgacact gaagtgatcc gcgacctcta cgttaccgta gtggcaactg
    16201 tcctgcgtag cgccgcactg gtgggcgcga tgctggtggc gatgttcagc ctcgactggc
    16261 gaatggcgct ggtggcgata atgattttcc cggtggtgct ggtggtaatg gtgatatatc
    16321 agcgttacag cacgccgatt gtccgtcgtg tgcgcgctta tctggcagat atcaacgacg
    16381 gctttaacga aattatcaat ggcatgagcg ttatccagca gttccgtcag caggcgcgat
    16441 ttggcgaacg tatgggtgag gccagtcgtt cacactatat ggcgaggatg caaaccctgc
    16501 gcctcgacgg ttttctgctg cgtccgctgc tgagtctgtt ttcatcgctc attctttgtg
    16561 gcttgttgat gctgtttggc ttctccgcca gcggcaccat tgaagtgggc gtgttgtatg
    16621 cgttcatcag ttatttgggg cgacttaacg aacccttaat cgaactgacc acgcagcagg
    16681 cgatgctgca acaggctgtt gttgctggtg agcgcgtttt tgagctgatg gacggaccgc
    16741 gccagcaata tggcaatgat gatcgcccgt tgcagagcgg caccatcgac gtcgataacg
    16801 tgtcatttgc ttatcgcgat gacaatctgg tgctaaagaa cattaatctc tctgtgcctt
    16861 cgcgcaactt tgtggcgctg gtcgggcata ccggcagtgg caaaagcacc ctcgccagtt
    16921 tattgatggg ctattacccg ctaacggaag gtgagattcg ccttgatggt cgtccattaa
    16981 gtacgctaag tcacagcgcg ctgcgccagg gcgtggcaat ggtgcagcaa gatccggtgg
    17041 tgctggcgga taccttcctc gccaacgtga cgctgggacg tgatatctct gaagaacgcg
    17101 tctggcaggc gctggaaatc gtgcagctgg cggagctggc gcgtagcatg agtgatggta
    17161 tttacacgcc gcttggcgag caggggaata atctctcagt cgggcaaaag caactgctgg
    17221 cactggcgcg cgtgctggtg gagacgccgc aaatcctgat ccttgatgag gcaaccgcca
    17281 gcattgactc cgggactgaa caggcgattc aacatgctct ggcggcggtg cgtgaacata
    17341 ctacgcttgt ggtgattgct caccgcttat cgactattgt tgatgccaac accattctgg
    17401 tgcttcatcg tgggcaagcc gtggagcagg gcactcacca gcaactgctg gcggcccagg
    17461 gacgatactg gcagatgtat caactgcaac ttgcgggcga agagctggca gccagcgtgc
    17521 gtgaagagga atcattgagc gcctgaatag cgcaatattt catcgttggt gcaaaaatgt
    17581 aacgcactgt gcactgtcat agtgcgtttt cattttcaat cttcttaact tcctgctctc
    17641 tttctcgttt ttcatttttg gcacaccgct tgcaatacct tcttcgtgta gcagaaccat
    17701 taccgaattc tgaccggagg ggatctatga agctggtgac cgtgattatc aaaccattca
    17761 aacttgaaga cgttcgtgaa gcgttatctt ccattggtat tcagggcctg accgtcaccg
    17821 aagtgaaagg tttcgggcgt cagaaaggac atgccgagct gtaccggggg gcggaataca
    17881 gcgtcaattt cctgccaaaa gtaaaaattg atgtggcgat tgctgatgac caactcgatg
    17941 aagtgatcga tatcgtcagt aaggcggctt acaccggaaa aattggcgac ggcaaaatct
    18001 tcgtcgctga attgcaacgc gtcattcgta ttcgtaccgg cgaagccgac gaagcggcgc
    18061 tgtaatctct ggcacacagc aacaggaacg aaaaatgaag atagcgacga taaaaactgg
    18121 gcttgcttca ctggcgatgc tgccgggact ggtaatggct gcacctgcgg tggccgataa
    18181 agccgacaat gcgtttatga tgatttgtac tgcgctggtg ctgtttatga ctattccggg
    18241 gattgccctg ttttacggtg ggttgattcg cggcaaaaac gtgctgtcga tgctgacgca
    18301 ggtgacggtg acgtttgcac tggtctgtat tctctgggtg gtttacggtt actcgctggc
    18361 gtttggtgag ggaaacaact tcttcggcaa cattaacggg ttgatgctga aaaacatcga
    18421 actgacggcg gtgatgggca gcatttatca gtatatccac gtagcgtttc agggatcgtt
    18481 tgcctgcatt accgtaggct tgatagttgg agcactggcg gaacgaatcc gcttctcagc
    18541 tgtgttgatt ttcgtggtgg tatggctgac gctctcctac attccgattg cgcatatggt
    18601 ctggggcggc ggtttgctgg cttctcacgg tgcactggat ttcgcgggtg gcacggtggt
    18661 gcacattaac gccgcaatcg ccggtctggt gggcgcgtat ctgataggca aacgcgtggg
    18721 cttcggcaaa gaggcgttta aaccgcacaa cctgccgatg gtcttcaccg ggactgccat
    18781 tctctatatc ggttggtttg gctttaacgc cgggtcagcg ggcacggcga atgaaatcgc
    18841 agcactggca tttgtgaata ctgtggtcgc aacggcggcg gcaattcttg gctggatctt
    18901 cggtgaatgg gcgctgcgtg gtaagccttc actgctggga gcgtgttcag gcgcgattgc
    18961 cggtctggtc ggcgtgacgc cagcgtgtgg ctacattggt gttggcggcg cgttgattat
    19021 cggcgtggta gctggtctgg cgggcttgtg gggcgttacc atgctcaaac gcttgttgcg
    19081 ggtggatgat ccctgcgatg tcttcggtgt gcacggcgtt tgtggcattg tcggctgtat
    19141 catgaccggg atttttgctg ccagctcgct gggcggcgtg ggcttcgctg aaggtgtgac
    19201 gatgggccat cagttgctgg tacagctgga aagcatcgcc attacgatcg tctggtccgg
    19261 tgtggtggca tttattggct acaaactggc ggatctgacg gttggtctgc gtgtgccgga
    19321 agaacaagag cgagaagggc tggacgtcaa cagccacggc gagaatgcct ataacgcgta
    19381 acaagcactg caaaaaacag ccggacggtt ttcacctccg gctatttttt taattatgat
    19441 tacgcattac cccttcctga acggtcgagg caaccagcac gccgtcctga gtataaaact
    19501 caccgcgcac aaagccgcgt gcgctggacg ccgaggtgct ctccacgcta tacagcagcc
    19561 attcattcaa attaaacggg cgatggaacc acatggaatg gtcgatggtc gctatctgaa
    19621 ttcccggttc gagaaaaccg atgccgtgcg gctgtagagc taccggcagg aagttaagat
    19681 cagaagcgta accgagcaga tactgatgaa cgcgcagatc atccggcacg ctaccatttg
    19741 cgcggatcca cacctgacga tgcggttctg cgacgtgacc tttcaatggg ttatgaaatt
    19801 ccaccggacg gacttccagc ggacgatcgc agatgaattt atctttcagc actggcggca
    19861 gcaggtgcgc cagcgattgg gcgatttgtg tttccgaagg caggccatca ggcgctggcg
    19921 cggacggcat tgttttttga tgttcgaaac ccgcttctgg tgcctggaaa gaggccgtca
    19981 tataaaaaat cggtttgccg ttttgaatag cagcaacccg gcgggcgctg aagctgttac
    20041 cgtcacgcag cgtttcgaca tcataaataa tcggcttctt actatcgcct gggcgaagaa
    20101 agtagctgtg aaacgaatgt accagccgct cttcagggac ggtctctttt gcagcataca
    20161 aggcctgacc cacgacctgg ccgccaaaca cctggcgtaa acctaaatct tcactctggc
    20221 cgcgaaagag tccttcctca attttttcca gatttaacaa tgtcagtaaa ttttttagcg
    20281 cctgactcat ataactctcc agtaacaaag ctgccgcagc aagccaaagt gagttgagta
    20341 taacgcaaat ttgccactgg tccgatgggt gcaatggtct gaattacggg ctaattacag
    20401 gcagaaatgc gtgatgtgtg ccacacttgt tgacgttact attttgttaa ccactcttcc
    20461 ggcgaggaaa gttagcccgc tggtgcattg ataataagga gaagtgaatg aaactcgtgc
    20521 acatggccag tggtttagcg gttgcgattg cgttggcggc ttgcgcagat aaaagcgcgg
    20581 atattcagac gccagcaccg gctgcaaata cgtctatttc agcaacacaa cctgctatcc
    20641 agcaactgaa tgtctccggt accgtctgga tccgtcagaa agtcgcactg ccgcctgatg
    20701 ctgtgctgac cgtgacactt tctgacgcgt cgttagccga tgcgccgtca aaagtgctgg
    20761 cgcagaaagc ggtgcgtacc gaaggtaaac agtcaccatt cagctttgtt ctgccattta
    20821 acccggcaga tgttcagccg aacgcgcgta ttctattgag tgcggcgatt accgtgaatg
    20881 acaaactggt atttattacc gataccgttc agccggtgat caatcagggc ggaactaaag
    20941 ccgacctgac attggtgccg gtgcagcaaa ccgccgtgcc ggttcaggcc agcggtggcg
    21001 caacggctac cgtaccttcg acttcaccaa ctcaggtgaa tccgtcttcg gcagttccag
    21061 ctcctacgca atattaagcg cagttaaccc tctccgcccg gagagggtta gtagttccag
    21121 cgataacgct gcaagtcgat ttgcccgctt cccgatacca tcacaccttc tgccagtaat
    21181 gcctgtcgtt gacgctgtaa atccggtccg gttagcgaaa ttgtgccgtg gcgattaacc
    21241 acccggtgcc agggcaaggt gctgccttcg gggagacgct ttaacacacc gcctacctgg
    21301 cgcgcggcgc gcggcgatcc cgccagtttc gccacatcac cgtaagtggt gacatagcct
    21361 tcgggaatag cggcgacgat ttgccagacg cgttggggaa atgaatcttc tttttccatc
    21421 ttttcttcct gaggtaattt ttcagcataa tctggaaaaa cgcccgagtg aagccgcatt
    21481 gcgcaagaaa ccagcatctg gcacgcaatg ggttgcaatt agccggggca gcagtgataa
    21541 tgcgcctgcg cgttggttct caacgctctc aatgggggct ctgttggttc tcccgcaacg
    21601 ctactctgtt taccaggtca ggtccggaag gaagcagcca aggcagatga cgcgtgtgcc
    21661 gggatgtagc tggcagggcc cccacccatt tctgcctccc cccgtttcgt caaaaaatcc
    21721 caacatggct aaactttaac cacaactgac gtcgcaagaa ttgtctggct gcgcagtacg
    21781 cttcggaggt atgtctgatg aagtatgttg atggttttgt tgttgccgtt cctgccgata
    21841 aaaaggatgc ctatcgggaa atggccgcta aggccgcgcc attatttaaa gagttcggtg
    21901 cgcttcgtat tgtcgaatgc tgggccagcg atgtaccgga tggtaaagtg accgattttc
    21961 gtatggcggt gaaagcggaa gagaatgaag aggttgtctt tagctggatt gaataccctt
    22021 caaaagaggt ccgcgacgct gctaatcaaa agatgatgtc ggacccacgg atgaaagagt
    22081 tcggcgagtc catgccgttt gacggcaagc gaatgatcta tggtggattc gaatcaatca
    22141 tcgacgagta ggtagcgtga cgggctgtgc ggcgtgccgt cagccctaaa attcacaaat
    22201 gttgctcggc ccagcgtaag aaatcctctt tcggtaatgc cttgctgtag agccagccct
    22261 gaccgtagtg cacgccatgc tggcgtaacc actcttcttg tttactggtt tcgattccct
    22321 ccgctaccat tttcagtttc agtgtttttg ccatttcgat gatatgcggc gtgacatttt
    22381 tatattccag cgcatcaacg aaagatttat cgatcttcag aatgtcgaca tccagatcct
    22441 gtaaataact taaacttgaa taccccgtac caaaatcatc gagataaatt tcatggcccg
    22501 cctcccggta gcgagaaatt atcggggcgc tggttttcgg atcggcaaac tcgcgttcag
    22561 tgagttcaag cgcgatctgt ctgggattaa cctgatagtg attgatcatt tcacgcagca
    22621 attgcgggat tttttccgag gtgagcacgg tggattcaag attgatcgaa atatgctgct
    22681 gcggatgctg acgcagccag tcgcccatat cttcgaagac gcttcttatg atcagtagcg
    22741 tcaacggctc agaaaggccc gtttgctgtg ccagcggaat aaaactatct ggtgacaacc
    22801 aactaccgtc tgtctgcggc cagcgcgcca gtgcctcagc accgacaatt ttgccattgg
    22861 ctaaggagac aatcggctga tagtgcacgc aaatatcacg attttcgata gcatcctgca
    22921 gccgatgatg cggtgactgg atacggcgca aaatacgcag cacaaacatc gctgccagca
    22981 ggccaatcac caacccgagc ggtaaccaaa taaagacttg ccgatgccag cctttctgca
    23041 acattttcgt tgaagaccag gtgatgatcg aaatattcat ttccgggaag ggcaggatat
    23101 cgtaaatgat tccattattt tcgatatgct caccgggtgt tttttgtagt ctggtaataa
    23161 ttccctgagc aatttcatcg ctgctggtta tcacaacgtt atgggcattg ccaataatgg
    23221 cggcatcaat ttgccatgag ctatagggaa tgacatcaat aaaggaagcg gggtcgatca
    23281 tgacgacata atgtgccgtt cccatggcaa ccatgtaacg gataatgcct aaatcgttat
    23341 gcgatgttaa ccagacacga taaccatctt tcgaaatttt acctggctcg gggaaggtat
    23401 cgggcgggct ttcatgctcc agagacgaac actggggaac gttgttatcg atataaacca
    23461 cttcctgaat atagcgataa ctgtaagata cccgacgcat ttccatgaga tgggcttcgc
    23521 tacaggcagc gccttgccat ctttccagct cttgcagcgc atctttccct tgtgtcgcca
    23581 ccttattggc ccgaatagcg acgcgggagg aatacgtatt cagctcttca ataaatgatg
    23641 tttctacctg ctgatgggcc agccagatgc ttaagccgac aggcagcaat actgaaagaa
    23701 tcagtactcc cgaaataagg ccgaccagat gtcgtgttct caccagcata tccttattaa
    23761 cacagtgtag ggaaagtata tctgattgtg gaaaaaggct ggcaggagat aaggaggtag
    23821 agccgggatt tcatgaaggg atgggtggaa acgaacgttg tcatttgacc aggcgcagag
    23881 ctttacgcct ggtatgtaac cctaacttaa gatgttgatt cggcgcgagc gagggtaaaa
    23941 atatcgtaaa aagatagttc acctttacgg acgatcattt tttgcagttg ttctttatga
    24001 acatccgcga cacgcggaga gttcaggatg gcttcaacta gcgcggcagg tacaaagcgc
    24061 gtgttgtacc attcgccgtt atagcaaacg cggaaatcca gcacgtcgat atcttcatag
    24121 cggtagcgtg ggtagacatc gaaaaagaag aaaccattaa gcaggataaa taatacaacc
    24181 agtagccaga cagagcctga aagcgtttcg gactgcaaca tcactgccag ggtggcaaaa
    24241 aaagcaacgt acataccgat aaacaacccc ggatgtttac ggataaaact gatactaaag
    24301 cggggtttgt tgtcgcgttt ttcgcgggta tttagagact caattgtttc ggtcagcagg
    24361 cgttgtattt cggtcatcgt ttgccttcgt ggtatctgca actttccagg gaacacccta
    24421 ttttaacgtg ggaatagtag agggaaagta acagatttac cacaattttg cataacagtt
    24481 gcgaaaaacc gattacaatt ttttaattat tctggctgga ttaccgccca cgacaacgtt
    24541 gtccgggaca tcttttgtga caactgcacc tgaggctacc acgacgttat caccaatggt
    24601 cacaccaggg ttaatgaccg cacgtccgcc aatccagacg ttattaccga tggtgacggg
    24661 tttccccagt tcagcaccgc tattacgtgc tacagggtcg atgggatgtg ttgccgtgta
    24721 gatatgaacg cctggtgcca acatacagtt atcaccaatg cgaatagggc agacatcaag
    24781 catcacgcaa tcgaagttgg cgaaaaaatt attgccgaga aaaatgttat aaccatagtc
    24841 acagcgaaac gttggctcaa tataagcctc tgtcacctga ccgaataaat cagcaagaat
    24901 ttgctggcgt aatgtgtgct cttccgccag ggaatgattg tatcggtgaa taagccgacg
    24961 agcgcgcagg cgatcgcgag ataacgcctc atctgccgag tgatacaact caccagcaat
    25021 catcttttct ttttctgtgc tcattactga cctccggttg ctgaaaatgc ggcaacacag
    25081 taagacgaaa agtcagtgaa gagaacgttc ccattacatc tgtgtgatga agatcacata
    25141 gggtatgcct tcatggcgaa agaattataa aactcttaat aaacagccgg ttatagctcc
    25201 gaaagcgagt tatttattag cgaataaatt tccatactga ggaagggatc ttgtcgtaca
    25261 gtttattcat ggtcaattcg gcgaggcggt gatctgcggc tgagtaaaat accgccagct
    25321 cattatctga taattcgtat ttatttttct cgataacacg ctccagcgtg tcaattgtct
    25381 ggcaacgacg taaacgcatt aaataatcgg tttttgttaa aggtttttcg gacatacttc
    25441 tacccatggt tgtaataatt ctaacaagat aaactcgcag gattctcttt cgtcgcattg
    25501 acaaaacaac ggaatagtcg attacctgac ttccgccatt tctgaagatc ctgcatatta
    25561 ataccataac tactgaacaa cataaaggtg tcatccagat attcgtcgat ctgctcaatg
    25621 agcttattgt cttcattata cttaattttg taattaagtg cgaaggtcgc aatatgctca
    25681 atcagttcat tcaactggag gttgatcgcc gaggttgggt cgttaaccca gccatgattg
    25741 ctttcttcaa ggtttgcaag acagtcatga tacagggttt cacagagaaa cttaagctgc
    25801 gcgatatcat gtcttttggg tgagtattca tccataacgc gtccccttct tagcggttga
    25861 actaacggac acctttcggg atggaaaaaa cttactgacc tggacttgcc ctctcgtttg
    25921 ttagcttaac tataagccac tctttgcagg ttttcatcgc attattacga aaaattacaa
    25981 ttaggaagga agcgggacag gactcacttt ttcatactag cactaacccg cagcaggtaa
    26041 aagcagtttg ctcgaactgt tcataacatt tcaggattat cgccagatga aattggtaac
    26101 actaatacca ggattgctct gaatatgacg taata
//
LOCUS       DAZUCC010000052        26033 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZUCC010000052 DAZUCC010000000
VERSION     DAZUCC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.052"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHY09_001880"
     CDS             complement(29..4489)
                     /gene="mukB"
                     /locus_tag="ACHY09_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFS3884859.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPYQAS"
     gene            complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHY09_001881"
     CDS             complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="ACHY09_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFS3884860.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHY09_001882"
     CDS             complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="ACHY09_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFS3884861.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHY09_001883"
     CDS             complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="ACHY09_001883"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFS3884862.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7414..8193
                     /gene="elyC"
                     /locus_tag="ACHY09_001884"
     CDS             7414..8193
                     /gene="elyC"
                     /locus_tag="ACHY09_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFS3884863.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKSSSGEPRQE"
     gene            complement(8170..9063)
                     /locus_tag="ACHY09_001885"
     CDS             complement(8170..9063)
                     /locus_tag="ACHY09_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFS3884864.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDTSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHY09_001886"
     CDS             complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="ACHY09_001886"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFS3884865.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHY09_001887"
     CDS             complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="ACHY09_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFS3884866.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHY09_001888"
     CDS             complement(10194..11426)
                     /gene="ycaQ"
                     /locus_tag="ACHY09_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFS3884867.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLGDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPEWNDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHY09_001889"
     CDS             complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="ACHY09_001889"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFS3884868.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     NAGQTLVMTEKDAVKCRAFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHY09_001890"
     CDS             complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="ACHY09_001890"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFS3884869.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14231..16495)
                     /locus_tag="ACHY09_001891"
     CDS             complement(14231..16495)
                     /locus_tag="ACHY09_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFS3884870.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLASLTVFACVLVFIPF
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVAHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSQLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFDVPVDNG"
     gene            complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHY09_001892"
     CDS             complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="ACHY09_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFS3884871.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHY09_001893"
     CDS             complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="ACHY09_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS3884872.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHY09_001894"
     CDS             complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="ACHY09_001894"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS3884873.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="ACHY09_001895"
     CDS             complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="ACHY09_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFS3884874.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(20719..22002)
                     /gene="aroA"
                     /locus_tag="ACHY09_001896"
     CDS             complement(20719..22002)
                     /gene="aroA"
                     /locus_tag="ACHY09_001896"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS3884875.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQDNYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNMMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRIAMCFS
                     LVALSDTPVTILDPKCTAKTFPNYFEQLARISQAA"
     gene            complement(22073..23161)
                     /gene="serC"
                     /locus_tag="ACHY09_001897"
     CDS             complement(22073..23161)
                     /gene="serC"
                     /locus_tag="ACHY09_001897"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFS3884876.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSNFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(23360..24052)
                     /locus_tag="ACHY09_001898"
     CDS             complement(23360..24052)
                     /locus_tag="ACHY09_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS3884877.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFIILALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESVDYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            24182..25942
                     /gene="ycaO"
                     /locus_tag="ACHY09_001899"
     CDS             24182..25942
                     /gene="ycaO"
                     /locus_tag="ACHY09_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFS3884878.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELAGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         6311 a         7087 c         6328 g         6307 t
ORIGIN      
        1 aaaaggcggc attgctgccg ccttcatttt aactcgcttg gtaaggcgct tcgtcacttc
       61 ctggaagcgt ttcagggagt tgcggcgcaa atcctcgcag gccgacgacg tgaacgtgtt
      121 cggtattctg gaagacttta cgcaccagtt tataggtggt gcctttttcc ggactgatat
      181 tttccggcgc tgcgatgatg agctgcattt gcaaacgttc acacaattca aacagcgtgg
      241 cgatagaacg agcatccagt cgcgctgctt catcgaggaa cagcaggcgg caaggagaga
      301 tatctttacc gcgcaggcgg cgagattcat cttcccagct ttgtaccacc atcaccagaa
      361 tcgacatacc ggtaccaatc gcctcaccgg tcgacagagc gccagattct gcgcgcagcc
      421 agccatcaga accacggtta acctcaactt ccatttccag atagttgcgg taatccagca
      481 gttcttcgcc aatggtctgc ggcgtgcgct gccccatatc aatctgcgga ttaagacgct
      541 gatacaattt cgccagcgct tccgagaagg tcagacggtt gctgttaaac agatcctgat
      601 gctgctcgtg ctgttcagag agcacatcca gcagcatggc gtgcgtttca cgcacgttca
      661 cattcagacg tacgctgttt acctgaccga acgatacgtt ctgcaaaccc tggttgagca
      721 tacggatacg gttctgttcg cgctgaatgg ttttgcgaat aatgttcgcc acgctgcggg
      781 aactgatcgc cagtttctgt tcacgggagg ttaactcttc ggtcagacgg ctaagttcga
      841 tctccatctg ttcgatagct tccaccggat catcggtacg aataatatcc tgacgaatac
      901 gttcacgcag atgctgataa accgccacga agaactgaat tttacgttcc ggacgttttg
      961 gatcttccga catccgcagc acgtcgcgca gatgttcgtt atccgccacc gccagacgca
     1021 gcgcacctaa cgccttatcc gacatggaac gcaaatcatc agcggagaga taagccagct
     1081 cacgacggtg taagcggcgt tcaacgccgt tatctttcac catgcgcatg actgcacacc
     1141 agcccgcttt cgcggtcact acctgctcgc gcatctcaaa gtaatcacgc tccagcttgc
     1201 gcagtttgcg ggtcaggttg tccatctccg cttcgcagaa ggtaagcgct ttttccagtt
     1261 gattgcggcg tgaacggtta ttgctcagtt gcgcgtgcaa ctcgtcacgg cgaatacgcg
     1321 cccgctcttc tgccccgcta tcagcacgca cgccgatatc ctgcaattca cgttgcagat
     1381 cgttgagtag ctcttttttg gtgtcgtaag aacttttcag cgaagccagc acctggttgt
     1441 actgactcaa ctgcgctgcg tgtccgcgca acgcttcacg cgcgcgggta cgctccgctt
     1501 ccgcctgttc cagacgttca cgcagttttt cgttgagatc gctgttaccg ctcagcattt
     1561 ctgccgagtc agagtagcta aagtgcgcac gacgctgcac cacttccgtc agggcaaacg
     1621 cctgctgacg ggcatcgcgc tgcatctgct gagagtacgc gtaatcttct tttaactgtt
     1681 cgaactgttc cgggtcgctc tgcaataccg aaacgatcgg ttccagtttc gccagttgat
     1741 tgccaaactg ctgaacaaaa cgcgcggctt cctgggcttc atccagacgt tcgcggattt
     1801 catcgacgcg atccgccagg ctgtcatcag ccaacaggtt gagacgcggc agaatgcggt
     1861 tcagcgccgt aacgccctct ttcgcctgct caaattgaat acgctgctgc tggttatcat
     1921 tttcatgatt actcagcgcc cgctccagtt cgacgcgacg gctgttcagt tgacggattt
     1981 cggcttccgg atcagactca aacgccaccg ccagatgact acctataaag cggctgaacg
     2041 cctgatgcag acgctgggtt ttctgtacat caaaagagag cgtggcgaag cgttcggaaa
     2101 gcacttcacg ctcggcatgg aggctttcaa tacggctttc acgcgcagca cgaccaaaca
     2161 gcggaacttc cggaaaacgt gagtaacgcc actgacgatc ggcgattttc accactaccg
     2221 ctttttccag ctcatcaacg ctgaacacgc tgtcatcgaa tgactgcgga tccccttcga
     2281 tcagatagag atcttccggg caatcggtca agccttccag gtgttcggtt acctgtgaca
     2341 gatctggcac cacgatggcg tggcgtgacg ggccatacag tgccgagaag tacggcgcat
     2401 cttccagact cacgtcgtca taaatttcgg acagcagcac gccgccaaaa cgctccgcca
     2461 gcgcattcaa acgctggtct tcagaaccac ctggctggct taaacgttcg atctcttcat
     2521 cgacggcgtt tttgcgcgcg cctacttcat cgcgttcaac aatggcttcg cgctcacgct
     2581 ccagcaactg ttgcagatat tcagtgacgt cctggctgga ggtgaactct tcgccgcact
     2641 gttcgctcaa ctggttgaga ctgttttgcg ctgccagcca aaccggtgca cgctgcatca
     2701 aactctgaat gcgagactgc agctgttcct gttcctggcg cagtgccatc cgctcttctc
     2761 gggcgttaga gacgctatcg gaaagagagg caatgcgtgc ttccagctcc tgatgcagag
     2821 cttccagttc atcaatatca aaattcttgc cctgacgttt gcagaaatct gccagcagac
     2881 gctcagcttc ttgctgctca cgcagacgct gttccagttc gcttaagcgc atccgcaacg
     2941 gctgaacctg ctctgccagg tgacgctgat cgaccccttc gcgcaatagt tcgcgagcga
     3001 catcccacgc ctcgttacgc gccagcgggc cgttgattgc caccaccagc tgataagcct
     3061 gctcaaactg gctgtgcgcg gtttgcgcca tgctcatttt ctgctcaaga gagagcattt
     3121 tttcggtcgc ttccagctct ttcgcctgga aggtttccag ccattcggca gcgctgtcgg
     3181 cggttaagtc cggcagatgg cacagttctt tggcacgatt aagcgcagca atcgcctggt
     3241 tatactggat cgcgcgcgtt tgctggacgt ccagcgcctg ctggtagtca gcaagctggc
     3301 ttttcagctc gtccacttcc agttcggcag cttccgcacg agcctcgttc tcttcctggc
     3361 gttcgatggc ttctgccacc acttcgtttt gctcttccag acggatctgc agctcatcga
     3421 gatccgcttc gtagcgttcg atcttctcct gctgacgcag tgcggtttgc accaggttca
     3481 ggtgatcgct ggccgcctga taatccgctt ccagatcgcc ttcggcaccg ttgtgctcag
     3541 ccagctctcg cgccatgtcg acgtgtttgt actgctcagc cgccagttgc tgacgcgaag
     3601 tatgtagctc gcgacgaaac tccagggcct tatcgagatg aacacggcgc tcgttggcgt
     3661 gacgcatgta gtccgccgcc acgtagttgg tggcttcgct gatcagatgc ttaaacaggt
     3721 cacggtctga ctgggtgaca cgaatcgctt ccagcgtcat gcggttttca cgcagcgccg
     3781 cttccatgtc ctggaacgct ttccgcaccc cactgttttc cggcaacagg tagtcgcgca
     3841 gagaacgagt aatggcgctg gagatcccgc catacagcga ggcttcgatc agacgataga
     3901 atttactacg gtcagacgct gagcgcagac gacgcgcgat gatgcccaga tcgaacatca
     3961 gcgagtggta atcggtaatg gagttgaact gtttaaactg cacgccttcc atcgcctcga
     4021 gcttgtcttt cagctcgtta agcggcagca cgcgcgcctg gcgttcgttc agagtttcgg
     4081 tcaccagttg tgtcggctgc accgacatcg gcaggccctg aatggcaaat ggtttgatat
     4141 ccactttacg atcgcgtccg gcaacctgtt gcagacgcac accgaccact acacgctggt
     4201 ggcgcgagtt aatggtgtcg agcatcgaat aacagacacc cgctttcagc ttaccgtgca
     4261 gacctttatc gcgcgaaccg ctggtggccc cggcttccgt agtgttacgg aaatgcagca
     4321 gggtcaggtc ggggataagc gccgtaacga acgctgccat ggtggtggat ttacccgccc
     4381 cgttaccgcc agaaagcgtc gtgaccagct cgtcaaggtc aaaagttcgg gcaaaaaagc
     4441 cgttccagtt aatcagcgtc agtgagcgaa atttaccgcg ttcaatcatt attcttcctc
     4501 tccgctatct ggctgactct cttcggtttc atcgttgagt tgcagatgat tttcaatcgg
     4561 cattgcttcg ccatcacgaa tcaggcgacg ctgtgcttca cggggatcgt cgccagcacg
     4621 cacatcggct ccgaagcgga acaccgattc ggtaatgcgg aacttgctgc tgtcgtggcc
     4681 cataaaccac accataccca aacgacgcag acggttgagc gaagaacgta ctttctcctg
     4741 caacttctgc cggtcaacgt ctgaaccggt tgaacggttg ttcaccagtt tcagcagttt
     4801 cgcttcatcg gccagggtga gcagttcgtc gtacagttcc tgctgggtga aaatcccctc
     4861 attcgccagc cgttccgggc tgagatagag ataacagagg attttcccga ccatcatatc
     4921 cagttccgac aagacggaac gagggatcag cgtggtggaa cgtgggcgta gatagaagaa
     4981 cccttccggt gcacgaataa gctcaacgtt ataacgcgcg taaaactctt ccagatattc
     5041 ctgaaaatcc atcaggaatg catgattatc cagttcgtcg aggccaatat gacggcctga
     5101 acgtaaggcg ctatccagcg ccggaaataa cggattcgcc agcgcctgcg ccagcttaac
     5161 cggcatcact tgttcaatat ttgtcgatga catgcgcctg taccttggct ccgtaatcat
     5221 taatcggctg ccatttcgct ggcagtccgg tgaaatctgc ttgcgctacg ccaagacgta
     5281 ccgcctgatc aataacaatg cgcgcaacgt caaagtgacg tgcacgcgga tactgtgaca
     5341 gatattcgcg taccaccaga ccaagatcca acggcacttg tctggttttg tacacggcaa
     5401 gttgttcttc gatgatcgcc gccagctgtt cgcggatctc gttaaactct tcgtactcca
     5461 gatcctcagg aagttcccca gtcacttctt catcgcgcag taccatctct tcgtcacgca
     5521 tatccagcag acgatcggca ttggcataag ttagcgccca cggctcatca aaataggttt
     5581 gtaccgactg acgtaaccgc tgagcaaaga cgcggttttt atccatatcg atcgcagtac
     5641 ggataaattt gtgtacgtgg cggtcgtagc caatccacaa gtctatggat tgctggcccc
     5701 aactgataat acggtcgagt ttgctctgca gatcgaacac cagacgatcg acaaaatgca
     5761 ggtcgtcatg ggtcatcgtc gcatcctgaa tgcgcaacag attagcctgc aatttatcgc
     5821 ctgccgcttc cagcgtatcc tgcaattcac gcagcgttcc ggaagtttct gaaagcaaca
     5881 attcacagct ggaaatcgcc gcccgccagt ctttgttcaa caactgggcg atatcatcct
     5941 tcacctgctg ctgctgttcg tccatcagac gttgcgtcag gtcgatactg tcgaaaattt
     6001 ctgctaccga atatttcagt ggcgcataaa cattacggtg ccagtgaaat tcatcaccgc
     6061 cctcttcggc ggcatctgcc gcgcgtttga gctcacccgc cacaatcgac aactgcatag
     6121 aaaggcgcag cgtagaaaac tcgcgctgac ggatgtaata gtcagtaatg ccgatgccga
     6181 gcggcgtcag acggtaaatt gcgttccctt ccgcctgctc gctggtaaag cggttcagca
     6241 gacgttgacg caccatgtcg ttgatcgcgt tgttggcgcg cacgccgatg gtttcgctgg
     6301 tttgctcaaa cgcatcactc acatggcgga atgcatccac cagctcgcct tcactcatct
     6361 caccatccag acgctcgccg ttcagcgtgg caaccgccag cagaaaagag agtcggtcta
     6421 ccggcagcga gatggagaag tcattttttc tggcccaggc aaccagttcg gggactgtct
     6481 gggaaaattc actcatactt tatccttgct ctgcggtttg cgcgcggtga catgaatata
     6541 acgccccagg gtaatatacg gttcctgacg gcaataacgc gtttctaatt caagtaatgc
     6601 ttcatagcag tcgcgctgct ggtgcttctc gcgcagataa tcatgaaaca cgcgaacgcc
     6661 tgtcttaccc ataatttgcc aaccagcttc ttccagccac agataaacct gcgccgggtc
     6721 gcgtggataa tctggcgaaa gcgtccgttt tttctttttc ggcattcccg cctgcacgta
     6781 atcaaaattc ccggcgacca tgttatgcat cagcaagccg tgcgcattgt agaacattaa
     6841 cgacaacaca ccgcctggac gcaacactga ccacagggtc tgtaatacgc tgcggggatc
     6901 ggccacccac tcgagcaccg catggaacaa tatcagatca acgggcgttt ccaaatgtga
     6961 agcaacatcc tgagcggcgc aatgtataaa ttgcatgttg tcgctcacac cttttgcttc
     7021 tgccgcctgt tttgcgcggt cgatcatctg cgcagaaaga tcgcataaaa tgacctgatg
     7081 cccgcgctca gccattttga ttgcggtctg cccttctcca ccgccagcat ccagcacacg
     7141 cagtttttgc gggcccattt ccgccagcac gcgatcgaga tcctgccaca gaatagcctg
     7201 tcgaagctgc cctttggtgg tgccgtaaat gttacgggaa aacttttccg caatatcatc
     7261 aaaattgcga tcctgcattg ctttctccac cggttggcta agacgttttc tgataaagac
     7321 gctattttgt cacagccacg gcgagaatga acccgtctgg tgtaatatcg ctggcgatcc
     7381 cttgctatat ggttaaaaaa ggaaccagaa aggatgcttt ttacactgaa aaaagtgatt
     7441 ggcaatatgt tgctgccact tccgctaatg ttgttgatta ttggcgctgg cctggcgctg
     7501 ctgtggttta gccgttttca aaaaaccggg aagattttta ttagcattgg gtggctggcg
     7561 ctcttgttat tgagtttgca accggtagcc gaccgcctgc tgcgtcccat cgaaagcacc
     7621 tatccgacat ggaataattc gcaaaaagtg gactatatcg tggtgctcgg aggtggttat
     7681 acctggaacc cgcagtgggc accaagctcg aatttaatca ataacagtct gccccgcctg
     7741 aatgaaggta ttcgcttatg gcgcgaaaat ccgggatcaa aactgatctt cacgggaggc
     7801 gtagcaaaaa ccaatacggt gagtacagcc gaagtaggtg cccgagttgc gcaatcgctg
     7861 ggcgtgccgc gcgagcaaat tatcactctg gatttgccaa aagataccga agaagaagct
     7921 gcggcagtga aacaggcgat tggtgacgcc cctttcctgc tggtgacatc cgcgtctcat
     7981 ttgccgcggg cgatgatctt ttttcagcag gaagggttaa atccgctgcc agccccggcg
     8041 aatcagttgg ccatcgactc gccgcttaac ccgtgggaac gggcaatccc ttctccggtc
     8101 tggttgatgc atagcgatcg tgttggctat gaaacgcttg gccgtatctg gcagtggcta
     8161 aaaagttcgt caggcgagcc acggcaagag tgattttgat gccagatcga agttgcgccg
     8221 actaaatcgt ccggtattaa ccagttgcgc aacttcatcc cataacacat acaaccaacg
     8281 ccgccagatg aacgactccg ccactggcgc gcgttgcagg taactccaga gcaaatcttc
     8341 cgccagagaa gtatccatta gtcggaacag ttcgtattcc cgcggtgccc aaagcattag
     8401 tcccggcccg accatcgcca gtaactgatc gctgcgcgaa tctttcagca tgctgcgtaa
     8461 acagaagtta ccgtgaatca gcacacaatt gtcgttaaag ccttcaaata atgccgggag
     8521 acattcgcga gtacgaaaca ggatccgctt atcctgcatc gttagaccgg tgttattgaa
     8581 ctgattgagc gtggtccata gcacttcaac atgttgccgg taccatgagg gccagaaatt
     8641 ttcctgagta ttgtcgaccg cgccgacgca accgcgactg tcctgacggt gccaggccag
     8701 taaggcttca acgatttgat ctttgagttg ttcccagcgt tctggtgttc gggctggtgc
     8761 ctccaccgaa acaccacgca tccgctccag tagcaggaca tcggggccgg ggtgttcttc
     8821 atgcgtcatc acaccataaa tagtcggcat acggacagtc ccacttcttg ccagcatggt
     8881 ggttttccat gccagttgtc gggcttttcc gggcgtacta aagcttcttg ccattaacgg
     8941 cattgggttt ccctggctgt catacaaagc ccacaacgcc gtatccgctt tttcattgac
     9001 gcactcaata cgactgagtt tttcgcccag taaatggctt aattcggcac gcagctgttc
     9061 cattcagatt accctcataa aagctactgc tttcataatg agcgccaata taaaagatgt
     9121 cacctgaatg agatcaattg ttgaaaagaa aaattgagga aaatcgtaac ggtacgacac
     9181 tcctcccaaa attggctgga gtgtcgtgaa gtgaaattag cgcatttcag cgcgaacgcg
     9241 ctcgagatct tcaggggtat ccacacctgt gccaggaact tcctgggcga cagcaacatg
     9301 gattttttcg ccgtaccaca gaacacgaag ctgctctaac atttcgatgt gttctaacgg
     9361 acttggctgc cagttgacgt aacgacggat aaagcctgca cggtagccat aaataccaag
     9421 atgacgcagg aagttatcgc caacggtttc aaggcctttt gcaaaacgat cacgatccca
     9481 aggaatggtg gcgcgagaga agtacagtgc atacccttca gcgtcgagga ccactttcac
     9541 cgcattcggg ttaaacgctt cctccgcatt gtggattggc accgccagag tcgccatacc
     9601 cacctgacgc tgagcgaggt tatcagcaac ctgacgaatg attgtcgcag ggatcatcgg
     9661 ttcatcaccc tgcacattaa cgatcacagt gtcgtcgctg aatgcgcatt tttcgacaac
     9721 ttccgccaat cgttctgtac ctgactgatg atcggcgcgc gtcatacata cttcgccgcc
     9781 agcggcttca acagcgcggg caacatcctc atgatcggtt gccacgatga tgcgctcggc
     9841 acctgattcg cgcgcgcgtt caagaacatg aacaatcatg ggtttgccgt taatatcaac
     9901 caatggttta ccgggcagac gcgtggacgc gtaacgcgcg ggaataatga ccacaaaact
     9961 catgatttac tctcatcagc agtcagcacg cgggcttccg tttccagcaa cactggaatg
    10021 ccatcgcgca gcgggaaagc caggttgtcc agtttgcaaa taagctcttg tttttcctgg
    10081 ttataccaaa gttttccgtt gcaaaccggg caggcaatga tttcaagcag acgatgatcc
    10141 atagttcctc cagatggatc gggttatgaa tgcataaatc ttatcataat catttatgcg
    10201 acggggtcta tttcccagcc ggcgcggcaa tcagtaaaga ggccttgcgg gcagcgtcct
    10261 aatgtcaccc gcgttgcctg ctgccagtta gcgaaatcag taatcgcctg acgtaaccct
    10321 ttttgcagcg ttgtcgttgg tttaatgcct tcttgtaacc acagagagat aacttcaagg
    10381 atgcctgtct ggcgatgcat tttggcatcc attcgcccaa ctaattgccc acgatgtaat
    10441 aacggcagaa caaaatagcc atactggcgt ttcggcgctg gggtatagca ctccagccgg
    10501 tagctaaaat caaaaagctg ctctgcgcgt ttgcgatccc agacaacagg atcaaaaggc
    10561 gaaagtaccg cgctgtgcgt tgcagtgagc tttccggcca gcgcacgctc gagtagcggc
    10621 agcaaatcag catgcagcca aagattgccc aatttttcaa catgcacagc aatgatttgc
    10681 tgctgttcag ccctcgcttc acgccacgcc gccagtgccg gacgttttag ccggtagtaa
    10741 tctgccagcc actgttcgcg aaatataccc aggctacgcg cactgttatc cagcatgatg
    10801 atttctgctt ctgcttgcga aacgagatcg cgctcatcat tccactcagg catgacacgg
    10861 tgagttaaat cataaacgcg ctggaagttg cgccgttcaa tcaccatcac ctttccggca
    10921 gtaaataaac cttccagatg tcgtttatgc ggcttccact cccaccagcc gctcgcccct
    10981 ttacgaggat gctcaaaatc ggctgaacgt actggcccct tatcatgaat atgctgaatt
    11041 aactgtgcaa tttccgccgc atgttcctgc atccaggcgt ctttatattt ccagcccatt
    11101 ttttcaggtg ccagcatgcg gtggcgaata agacgaaaat cgctacgcgg cataaagcag
    11161 gcttcatgcg cccagtattc cattaattcg ccacgcgcca gagactcatc cagccactgg
    11221 gcaggataat ttcccagacg actgaaaagc accagatatg gactacgggc aacaatattg
    11281 atggtatcga tttgcagcaa ggacatgcgg gagatcgttg ccggaatatc ccccaacgac
    11341 gctcgacggc ggggtttgtt taacaggcct tgcgcggcga ggtgaagatt acgcgcatca
    11401 gcaagagaga ggtgcggcag cgacattcat gactccatca atcgaacgct gccgcggcgt
    11461 aactagttgc cagaagccag cgaggttagt tgcgcaagca gtttcgctgg ttcatcacct
    11521 gaaagctgtg cgtcaacagg caaataccac caatttcctt cggcaaaggc ccggcatttc
    11581 accgcatctt tttcagtcat taccagcgtt tgcccggcgt ttaccaacgc gctgacatcc
    11641 gcatggttca aagactgatg atcggccagc ggtacacatt tttccggttg tacgccgcac
    11701 atcttcagcg tggcaaaaaa gcgcggcgga tgtccaatcc ccgccatcgc cactacatgt
    11761 tcaagctgag caacgtcaca acgcgtaccg gtacgtaaat tcaccgcctg acccggcagc
    11821 agatgcatgg ggatttcacc gctgcggggg acaccgccgt tgacgattac cgcatcaacc
    11881 gactttaagc gccccgctcg ctcacgcatt ggccccgccg gcaaccacca gccattgcca
    11941 aagcgacgca caccatcaat aacgacaatt tccacatcac gcgccagacg ataatgctgt
    12001 aaaccatcgt cggttacgat gatctgcaca tcagggtgtt gcgccagaat ggcttttacc
    12061 gcgtcagaac gcacgggaga aaccgcaaca ggcgcgccag tgcgttgata aatcaacaca
    12121 ggttcatcac ccgcctgtgc tgtggtggta tctgccgaca ataacagcgg ataagattca
    12181 gccttaccac catatccccg cgatacaacc cccacgcgaa taccgcgctg ttgcagctgt
    12241 tccaccagcc agacaacgac cggggttttt ccgttgccgc ctgcggtgag attaccaacc
    12301 acgacaaccg gtacgggggc acgccaggcg cgcttcagtt ttagtttata gcaaagacgg
    12361 atcgcgccac tcaccaggcc atacaaccag gagagtggca gcaatagccg ccataaaggg
    12421 gattcaccag accagatttt ttcgatcatt ggccaaactg cattttgtga agttgcgcgt
    12481 aaacgccacg gtgctcaagc aaatcgttat gcgtaccgcg ttccacaatg acaccatcct
    12541 cgacaaccac gatttcgtct gccttttcaa tggtagacaa gcggtgggca atcaccagag
    12601 aggtacggtt tttctgcaac tcatccagtg ccgcctgaat cgcacgttcg gattcggtat
    12661 ccaacgccga ggtagcttcg tccagaatca gtatcgggct atcacgcaac aaggctcgag
    12721 cgatagcaat acgctgacgc tgaccgccag agagcagcac gccgttttca ccaatcactg
    12781 tatcgagacc gttatccatc ttattgatga agtccatggc gtaggccata cgcgccgctt
    12841 cttcaatttg ctcacggctg tactgttcag tccgtgcgta agcaatgttg ttagcaaccg
    12901 tatcgttaaa cagatggaca ttctgcgaca ccagagcaac ctggttacgt aacgacgcca
    12961 gggtatactc gcgcagatcg tgaccatcca tcaggatttc gccttcatca atatcgtaaa
    13021 aacgcgtgat caggctggcg atggttgatt tacctgaacc agagcgtcca accagagcaa
    13081 ccgtcttccc tgccggaatt ttcaggttga tgttacgcaa tgcgggcaca tcacgtcccg
    13141 gataagtaaa ggtgacattg cggaattcca cgtcgccagt cgcacgctcg atcacgcgct
    13201 taccttcgtc tttctcctgc tcactgtcca gaatggtaaa cagcgtctga caagccgcca
    13261 taccgcgctg gaactgggcg ttaacgttgg tcagcgattt cagcggacgc ataagtgcaa
    13321 tcattgaaga gaaaacaacg gtaatcgtac cggcagtcag gctatccatg acacttggga
    13381 agcttgccgc atacagaaca aacgccagcg ccaaagaggc gatcagctga atgatcggat
    13441 cagagatgga agaggctgaa accattttca tcccctgaag acgcattcgg ttgctgactt
    13501 tatcgaagcg tttcgtttcc acttcctgac caccgaaaat caatacttct ttatggcctt
    13561 tcagcatttg ttcggcgctg gtggtcacct gccccatggt gttctgcatg tttttactga
    13621 tgttgcgaaa acgcttcgat acaacgcgaa tcgcaatcga aacaatcggt gccagcacaa
    13681 tcaaaatgat cgacagttgc caactgtaat agaacatcat gatgaacagg ccgatgatcg
    13741 acgcaccttc acgtacaaca gtaatcagtg cgccggaaga agaagaagcg acctgttcgg
    13801 aatcgtaggt gatacgtgac aacagcgtac ccgttgactg tttgtcaaag aatgaaaccg
    13861 gcattcccat catgtgacca aacaggcggc gacgcatagt cattaccacc tttcctgata
    13921 cccaggagat acagtagctg gagatatagc tggtgatacc acgtaaaatc atcagcccga
    13981 tcaccaccag cggcatccac atcagcacgg agcgatctgt tttaccaaag ccatcatcaa
    14041 gaagtggctt aaggagcgat aacatgaagg tatcgctggc tgcgttgagg attaacgcta
    14101 cgcccgccac gatcagaccc gctttaaaag gcgcaatggt tggccacagt cggcggaatg
    14161 tctgccacgt agagagatct ttgtcgttat gcattcaaaa aaccagcatt tgttgaaata
    14221 gccgcatatt ctacccgtta tccactggca cgtcaaacca ctgatgatac caacgaggta
    14281 aaatttgctc tctgaggctg ctaatccgcc agccttgcgc tgaaaaattg accgttattt
    14341 gaccctgatg tggagtatca agccacttat aaccctgttg ttgatagcga tgtttaactt
    14401 tgcttgaggg cagtcgccat gcgttatagc gcgatgccga tgcgagtgcc acttttccat
    14461 tcactcgctg aattaatggc aacgatgatg aggtattact gccatggtga ggtacctgaa
    14521 gtaatgttgc ctgcatttgc tgccagtaac ggcttagcat cttttgttca gcgggggctt
    14581 caatatcacc ggttagaaga atgctattcg tcccgtcatc aatcttaacc acacaggaat
    14641 ggttatttcc tttatcgttg ctaccttgta aaggccagtg cgcgctgaaa cgcaatcctt
    14701 gccattgcca cgcttcgcca cgcacacagg gctgatgatg ttcccagttt aactgacttc
    14761 ggatccataa catcggccat gtgtgcaata ttgagtccag ccctccccgg tgatccagat
    14821 gttcatgact caaaataacg ccttccggtt caagattatg ccagtggagc caggggataa
    14881 tcagttgttg cccactatcc ccttcgggcc aggccagtcc cgtgtcatag agaatcgctt
    14941 tgccgtttcg cgcaatgacc attgccagcc cttgcccgac atcaagcatg tacacttgcc
    15001 actcgtcagg tcgaggtttt tgccacagcg gcaagcacat cagcaagcct acagccacac
    15061 acattgctgg caacgttcgc caggcgttta atcgccatac caccagtaag aaccatgggg
    15121 aaaatgttag ccattgccaa cgttcagcaa tgttgatcca cccttccggc aaactcttta
    15181 acccccagaa aagtaaagcc aaagaccggt cggcaagaaa ccataaccct tgttccagaa
    15241 taaacggccc gcttaaatgc gcaaccatcg cggcgaggat caacggaacc gtgataaatg
    15301 tcaccagggg aattgcaaat agatttgcaa taaacgaggt cagactaatg ccatgaaata
    15361 tgacgatttg cacgggcata agcaggagtg tgattcccag ttgtaaatga atgagggaaa
    15421 caactgcacg caataccggc ggcagttgcc actccggaca gggaaaccac tggtaccaaa
    15481 aaatcagtgc cgcgaccgca gcggcagaga gccataaact ttgcgagaga atggcaacag
    15541 gatccatcag caaaattgcc gccagacaac atatccatac atcccagcca ctccattgtc
    15601 gcccacttaa cttaagcatt ccccacatgg caagcgccac cacggtacgc aatgcaggag
    15661 gttgcatccc agtcagccag gcataaaaag cagcacagca gattccgcca attaatggca
    15721 tttgccagtg gatccagcgc ccaggcagaa aaacttgccc accgcgaatg agtccggcag
    15781 ccagcaacgc cgcaaaagcg atatgcaatc ccgatatcgc cattaaatgc gccgttccag
    15841 tatcgcgcat gatatttttg atttctttgg gaacggataa ccgttccccc atacctaaac
    15901 caagaataac cgcgttccac atatagggtt gcagcgttgt ttgtagtgac gccagatact
    15961 gtgcacgcag gctacaattc ggttcaatga cacttgcctg cagaaagcgg ccggtgagcg
    16021 gctgatgctg ggcaatggca taacgctggc tatcaaagcc gccatcatta agttgaccat
    16081 gaactgcacg aactttgagt ttcattgacc acagttgtcc agcacaaacc gcttgcggaa
    16141 gatattcgcc atacagcacg aggcctggcg cagggaatat acgtcgacct tgtagatgag
    16201 taatttgacc gtaatgagtg gtcataccgt cagtagcagt gatctcaaca attgcatcct
    16261 gcgtcgcgcc agttaaggtt tctcctgccc acaaaatttg ctttgctgct aatatgcccc
    16321 aaacgaaaaa cagcaacgtc agcgcgatat aacggacagt tttaaaaggg ataaatacca
    16381 gtacgcaggc gaagacggtc agagacgcaa gggttaatgt cccaggcaat tggggcaaaa
    16441 tcagcaacgg aaaaattccg caaattatgc atacgccgac tgtcgttatt ttcatgcacc
    16501 cctccctgtt tatggaaagt gtgcaacttt gttgagcggt tgtctggtgg gagttttgcg
    16561 ttccccgagg cacgttcaga agaatttaac gagagggtga aaactgttca aaactctgcg
    16621 aggctgcttc cagttccaga ttagagaaat gaaaaaagca cccgacaggt gcttttctcg
    16681 cgttcaagtt tgagtaaaaa cttaaccgta aatattggcg cgatcgcgca gttctttacc
    16741 tggtttaaag tgaggaacgt attttccttc cagttctact ttatcgccag tcttcggatt
    16801 acgtccggta cgtggtgcgc ggtagtgcaa agagaaactg ccgaaaccgc ggatttcaat
    16861 acgctcgccc tgcgcaagag tcgaggccat atgctccagc atctctttta ctgcatcttc
    16921 aaccgtcttg gcgggaatgt gcgattgctg ggtggcaagt ctttctatca attctgactt
    16981 ggtcatgatt cctccggttc cttaaaggca aatttagcgg ctgctgttgc tgcattgatt
    17041 aagggcggcc gtagccgccc ttagtgcttg attacaggac gaaacctgca atctgtcaag
    17101 taaactcaac aaacttcgga ataaaaatcc cgaagagtca gagaattact cgcctttagc
    17161 tgctttgaaa gcttcagcca ttgcgttgtt ggagaagttt gcatcttcct gtttgttaac
    17221 agttgcgatt gcatctttct cgtcagcttc gtctttcgca cgaacagaca ggctgattgc
    17281 gcggttttta cgatcaacgc cggtgaattt agcttcaact tcgtcgccaa cgctcagaac
    17341 cagggtagcg tcttcaacgc ggtcacggga tgcttcagaa gcacgcaggt aaccttcaac
    17401 gccgtcagcc agttctacgg ttgcgccttt agcgtcaact gcagttactt taccggttac
    17461 gatagcgcct ttcttgttca gagcaaccca gttgttgaac ggatcttctg cgagctgttt
    17521 aacgcccagg gagatacgtt cacgttctgc gtcaacctgc agaacaactg cagcgatttc
    17581 gtcgcctttt ttgtattcac gaactgcttc ttcgcctgca acgttccagg agatgtcaga
    17641 caggtgaacc aggccgtcga tgccgccgtc caggccgatg aagataccga agtcagtgat
    17701 agacttgatt ttaccttcaa cacggtcgcc cttgttgtgg gtttccgcga actgctgcca
    17761 cgggttagct ttgcactgtt tcagacccag ggagatacga cgacgttctt cgtcgatatc
    17821 cagaaccata acttccacta catcgccaac gttaacaact ttggacgggt ggatgttttt
    17881 gttggtccaa tccatttcgg aaacgtgtac caggccttca acgccttctt cgatttcaac
    17941 gaagcagccg tagtcggtca ggttggtcac gcgaccagtc agtttggtac cttccggata
    18001 acgtttagcg atagctaccc acggatcttc gcccagctgt ttcaggccca gggatacacg
    18061 ggtacgttcg cggtcgaact tcagcacttt aacagtgatt tcgtcgccca cgttgacgat
    18121 ttcgctcgga tgcttaacgc gtttccaggc catgtcagtg atgtgcagca ggccgtcaac
    18181 gccgcccaga tcaacgaatg caccgtagtc agtgaggttc ttaacgatac ctttaacttc
    18241 catgccttcc tgcaggtttt ccagcagctg atcgcgctct gcgctgtttt cggattcgat
    18301 aacggcacga cgagaaacaa caacgttgtt gcgcttctga tccagcttga ttactttgaa
    18361 ttcaagctct ttgccttcca ggtgcagagt gtcacgcacc ggacgaacgt ctaccagaga
    18421 acctggcagg aacgcacgaa taccgttcag ctcaacagtg aagccgccct taactttgcc
    18481 gttgataaca ccggtaacag tttcagcatc ttcgtaagct ttttccagcg tgatccaggc
    18541 ttcgtgacgt ttagctttct cacgggacag cagagtttca ccgaagccgt cttctactgc
    18601 gtccagagca acgtcaactt cgtcacctac ctggatttcc agctcgccct gggcgttttt
    18661 gaactgctca gccgggatgg cggactcaga tttcagacca gcgtcaacca gtactacgtc
    18721 tttgtcgata gcaacaacaa cgccacgaac gatagaaccc gggcgggttt cgatttcttt
    18781 taaggactct tcaaagagtt gagcaaaaga ttcagtcatg tttaatcttc aggtttatat
    18841 ttaacgtcca cctggctcca tgccggatgg ggttgtttaa catacccgct gtcattccat
    18901 tgcaacgggg gtactgcaaa ttcggtcgct tatgcgagag ccaatttctg gcgcgcgtat
    18961 tgtagcgctt tttcaatcac ttgctcaatg cttaaggtgg tggaatccaa cactaaagca
    19021 tcggctgccg gaaccagtgg cgctaccgct cggttacgat cgcggtcgtc gcgttctttg
    19081 atctcagcca aaaggcgctc aaagttaaca ctaaagccct tctcctgcaa ctgtagcatg
    19141 cggcgatgcg cacgttcttc cgaggaggcg tcaaggaaaa ttttcactgg cgcatcaggg
    19201 aataccaccg ttcccatgtc acggccatcg gcaatcaggc ctggtaattc gcgaaacgcg
    19261 cgttggcgac gcaataatgc ttcacgaacg cgtgggaatg ccgcgacttg tgaagctgca
    19321 ttcgccactt cctgagtacg aatttcgcca ctgacatctt ccccttcgag gatcacttcc
    19381 agattgccat tggtcgacac aaaacgtaca tccagatggg atgccagcgg caccagcgca
    19441 tcttccgatg caacatcaac atggtgatgt aatgccgcca gtgccagtac gcgataaatt
    19501 gcacccgagt ccagcagatg ccattgcaac gcttccgcca tagccttaca caaggtgcct
    19561 ttccctgcac cgcttgggcc atcaatggta ataaccgggg caattgccgt catctttatc
    19621 tccttaaaac aggcgtaccg ttaacataac cgcgcgcatt atacgcgccg acgccacaaa
    19681 tcgttaacgt tgtgtgcaaa aaccagtttt gtctgtattc agatggggat ttattgcgca
    19741 attatagcgg gctggcagaa gaccagcccg aaagatgaca atgatttact taccagaggc
    19801 gatacgatca cggatgtgtt gcgcacgctc tgttgatggt gggtgagaat caaacatgga
    19861 ctgggtacga ccgccatcca ggctggccaa tttttcaaag ctgccaacca gcccctgggt
    19921 gctgatgcca cgtttcttca acagatcaaa ggagaaatca tctgcttcgg actctttatc
    19981 gcgggagtat ttagcattga tcgcgccttc tgcgatatca cctaattgtg agcgagaaag
    20041 ctgggaagcc acaccgctgg tagcggaaat cgcatcacgt gcggcaacga tcgcataaga
    20101 agctttcatt tcagccagcg agtgacccaa cgcgacgtgg cccagttcat gacccagaac
    20161 gccttcaatt tcgttgtcgt tcatcatgtc catcaggcca ctgtagacac gaacacaacc
    20221 gttcgccatc gcccatgcgt tgacgtcgct ggtcatataa accttatagt tgaccggcgt
    20281 gccattaata ttgttaccca atgctttggc gatttttgcc agacgtttcc cgtacttgct
    20341 ggatttgctt gcgacttgat tgccgctgtc catttcggca cagccctgat tggcaatcgc
    20401 ttttgcatca gcatcgctta acgttgctgc tttataggca tttaaacctg agctgatagc
    20461 catattggta tcaataccgt gggtattttg acaccctgtc agtaatgctg cagaggccgc
    20521 aatcgccagc agtaatttag tattcttcat gtgaatcatc cgttgattaa tcatatataa
    20581 atattatgta tcaataactt acattgacaa gcgacgctac cattaatcaa aatgactcac
    20641 aaggtccgaa aaaaaacgcc ggcaatgtgc cgacgttttg ataaagaaag atttggctat
    20701 ttattgcacg ttgttcattc aggctgcctg gctaatccgc gccagctgct cgaaataatt
    20761 cggaaatgtt ttggccgtgc atttgggatc aagaatcgtc actggtgtat ctgacaacgc
    20821 caccagcgag aaacacatcg ctatccggtg atcattgtat gtcgcgatct cggcaaagtt
    20881 cagcttttcc ggtggagtga tacgaatgta atcgtgcccc tcttctactt ccgcaccaac
    20941 tttacgcagt tctgttgcca tcgcaaacag gcgatcggtc tctttaacac gccagttata
    21001 gatattgcgc agcgtggtgg tgccttttgc aaataacgcc gccgtggcaa tggtcatcgc
    21061 cgcatcggga atatggttca tatccatatc aatagcgttc agttcacccc gcgtgcagga
    21121 aatataatca tcgccccagc aaatggtcgc gcccattttt tccagcacat cagcaaagcg
    21181 aatatcaccc tgcatactgt tgcgtccaat gccggtcact tttacagtgc cgcctttgat
    21241 tgctgctgct gccaggaagt aagaagccga agatgcatcg ccttcgacca aataagtacc
    21301 cggagactga taagactgcc cgccttttac gacaaattgt tgatagtgct gattttcaat
    21361 ttcaacacca aacgtcttca tcatattgag tgtgatgtcg atataaggtt tagaaaccag
    21421 atcgccttta atacgaatca ccgtatcttc cggtgcaaga ggcgcagtca ttaatagtgc
    21481 ggtgaggaac tggctggaaa cggagccatc aacgtcaacg ttgccgccag taaagccgcc
    21541 ctgtaaacgc aatggcggat aattgtcttg ttccaggtaa gtgatcttcg ccccgcccag
    21601 gcgcagcgca tccaccagat gaccaatcgg gcgctctttc atacgcggct caccagtcag
    21661 cacaatatca ttgctaccca gacaaagagc tgccgccagc gggcgcattg ccgttccggc
    21721 gtttccgagg aacaactcca gggcaccttc tgcgtgtaat ggaccgccgt taccgataat
    21781 ttcgcaacgc gtacgatcgg ctgaaagcgt atagcttacc cctaacgctg ttaatgcatt
    21841 cagcatatgg cgcacgtcat cgctatccag cagattggtt aatactgttt tgccgtgtgc
    21901 taatgccgcc agcaataaag cgcggttaga aacgctcttg gaaccaggca gattaatagt
    21961 gccatcgaca cgagcgatgg gttgtaacgt cagggattcc atgaactcaa ctctctacaa
    22021 cagaaataaa aaccccacag gctggctgtg gggattaagc aaaatttcgg cattagccgt
    22081 gacggcgttc gaactcaacc atgaagtctg tcagggcttt aacgccttcc agcggcatgg
    22141 cgttataaat agacgcgcgc attccgccga ccacacggtg acctttcagc gcatgaaggc
    22201 cagcagcaaa agactcttca aggaacaatt tgtcaagcgc actgtccgcc aactggaacg
    22261 gcacgttcat ccgcgaacgg ttagctttcg ccacgtcatt gcggtagaaa ttgctgttat
    22321 caatcacccc atacagcagt tctgcttttt gctgattgat tttatccatt gcagctacac
    22381 cgccgttcgc tttcagccat ttaaagacca gaccagacag ataccaggca aatgtcggcg
    22441 gcgtgttaaa catggagtcg ttatcgttga gaatggaata atccagaatc gacggacacg
    22501 cgatattcgc tttgcccagc aaatcttcac gaacgatgac gattgtcagg ccagccgggc
    22561 cgatattttt ctgcgcgcca gcgtaaatca cgccataacg gctgacgtca atcggacggg
    22621 aaagaatggt tgaagagaag tcggcggcga ccaccacatc tttgccgaag tctggcgttt
    22681 cgtcgatagc gataccgtcg atggtttcat tcgggcagta atgcatataa gcagcattat
    22741 cagagagttg ccattcgcgc attggcttaa ccgcgcgcag accatcaaca gtcactttgg
    22801 cgtcaaagac attaggcgtg cagtattttt tcgcttcttt aatggcactt gccgcccagt
    22861 aaccggcatc aacataatct gcggtggttt tatcaccgag aatattcagc ggaacagcag
    22921 caaactgacc gcgaccgccg ccatggcaga acaatacctt gtagttggag gggacattaa
    22981 gaagatcgcg aaaatccttc tcggcttcct ctgcaacctg aatgaactct ttgccacggt
    23041 gactcacttc catcaccgac gtaccaagac cgttccagtc gcgcagttcc tgttgagcct
    23101 gtttaagcac ctctgccggt agcattgccg gaccagaact aaaattgaag atttgagcca
    23161 tttcccctca ccacgttgcg ttgctattaa cccgcggtca atcgattgcg accacaggta
    23221 atacattatt cctgtggcta tcggttttat cattcagtga cacacatcgc aatgagtaaa
    23281 accgttgagg gcaaaaatgt ggcttgcatc acacaaaaga atctctcgga ttattagcaa
    23341 aaagccgatg tttttactgt cacgtcaccc gttttgcccg acttgccttc gtccattctg
    23401 gatttgcaca gcgcggacat aattgttttt cccccgcgtt catatgaatg atttcactgc
    23461 aacgaatgca ggcatagtcc accgactcgg gtaccacttt gtaacgctga gtacagtcac
    23521 tgggtaaaat aagtaagccc ggtttcacct tgtcatcttc aaagaaatag acactgattt
    23581 gcccgttggt ttcgagaatc gccagacgta cttgccccaa ctgctctaca ccgcgcaatc
    23641 gtagctccat aaagaactca aattccgtca tgttggagtt attgagtttc gaccaggcca
    23701 gctcgccatc ttcaataatg acaaccggct tgccttccag aagatcttcc agtttttcac
    23761 tgtgcgccat caaccacatt accaggcggt ataacaacgc cagaataata aagacgataa
    23821 gtaccgggac catcggcaca tcatcataaa acgccacatc tcccgccgct gatcccagcg
    23881 tcagaatgat taacacttca aaaagcgaca tctgccgcac accgcgtctt ccggtcattt
    23941 tgaggaacaa aaaaaccagt acaaaggtat aaagactacg tagtgccact tcgccaagga
    24001 aatcaaaagg cactttatca aatgccatac ggtggagatc gaacgccttc atttatatgc
    24061 tcttatcgtg gtttatccgc taaaagcata gtcaacagat catcggcatg gcggcggtga
    24121 tagcgccttt gagataaaac ccgtattatt gcgcgctttc cgtacgacta aagtgatttt
    24181 catgacgcaa acatttatcc ccggcaaaga tgccgcactg gaagattcca tcgctcgctt
    24241 ccagcaaaaa ctttcagacc tcggctttca gattgaagag gcctcctggc tgaatccggt
    24301 gcctaacgtc tggtctgtac atattcgcga caaagagtgc gcactgtgtt ttaccaacgg
    24361 taaaggcgca accaaaaaag cggcgctggc ttctgcactc ggtgaatatt tcgagcgtct
    24421 ctcaaccaac tacttttttg ctgatttctg gctgggcgaa accatcgcca acggtccgtt
    24481 cgtgcattat cccaacgaaa aatggttccc actgagcgaa aatgacgatg tgccagaagg
    24541 gctgctcgat gaccgtctgc gcgcatttta cgatccggag aatgaactgg ccggcagtat
    24601 gctgattgac ctgcaatccg gtaacgaaga tcgtggtatt tgcggtctgc cgtttacgcg
    24661 tcagtctgat aatcagaccg tttatattcc gatgaatatc attggtaacc tgtatgtttc
    24721 taacggtatg tccgctggca atacccgtaa cgaagcacgc gttcaggggt tgtccgaagt
    24781 tttcgaacgc tacgtgaaaa accgcattat tgctgaaagc atcagcttgc cggaaatccc
    24841 ggcagacgtg ctggcgcgtt acccagcggt ggttgaagcc atcgaaacgc tggaagcaga
    24901 aggtttccca atctttgctt atgatggttc gcttggcggc cagtatccgg tgatttgcgt
    24961 ggtactgttc aatcctgcta acggtacctg ctttgcctct ttcggtgcgc atcctgattt
    25021 tggcgtagca ctggaacgta ccgtgaccga gctgctgcaa ggtcgtggcc tgaaagattt
    25081 ggatgtgttt actccgccaa ccttcgatga tgaagaagtt gctgaacata ccaacctcga
    25141 aacgcacttt atcgattcca gcggtttaat ctcctgggac ctgttcaagc aggatgccga
    25201 ttatccgttt gtggactgga atttctccgg cactacggaa gaagagtttg ctacgctgat
    25261 ggctatcttc aacaaagaag ataaagaagt ttatattgcc gattacgagc atctgggcgt
    25321 ttatgcttgc cgaattatcg tgcctggcat gtccgatatt tatccggctg aagatctgtg
    25381 gctggcgaat aacagtatgg gcagccattt acgtgaaacg attctttcgt taccaggcag
    25441 cgagtgggaa aaagaagatt acctgaacct catcgagcaa ctggatgaag aaggttttga
    25501 tgactttacc cgcgtgcgtg agctgttggg tctggcgacc gggtcggata acggttggta
    25561 caccctgcgt atcggtgaat taaaagccat gctggcgctg gctggtggcg atctggaaca
    25621 ggctctggtc tggaccgaat ggacgatgga gtttaactca tcggtattca gcccggaacg
    25681 cgccaactat tatcgctgcc tgcaaacgtt gttattactg gcgcaggaag aagatcgcca
    25741 gccgctgcaa tatctgaatg cgtttgttcg catgtacggc gcagatgccg tggaagccgc
    25801 cagtgcggca atgagcggcg aagcggcgtt ttatggtttg caaccagtag atagcgatct
    25861 gcacgcgttt gctgcacatc agtcgttgtt gaaggcctac gaaaagctgc agcgcgccaa
    25921 agcagcattc tgggcaaaat aaaatcaaat agcctacgca atgtaggctt aatgattcgt
    25981 ctgagttata ttacggggcg tttttttaat gccccgcttt acatatattt gca
//
LOCUS       DAZUCC010000053        25896 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZUCC010000053 DAZUCC010000000
VERSION     DAZUCC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.053"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1633
                     /gene="yqiK"
                     /locus_tag="ACHY09_001900"
     CDS             <1..1633
                     /gene="yqiK"
                     /locus_tag="ACHY09_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS3884879.1"
                     /translation="WMFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVM
                     SGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGI
                     ATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAE
                     DLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVE
                     VAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEAERRREAEQTR
                     ILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQ
                     SQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKA
                     EKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALP
                     AVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQALSAALSYRTQA
                     PLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            1873..1932
                     /locus_tag="ACHY09_001901"
     CDS             1873..1932
                     /locus_tag="ACHY09_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS3884880.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(2052..3485)
                     /gene="hldE"
                     /locus_tag="ACHY09_001902"
     CDS             complement(2052..3485)
                     /gene="hldE"
                     /locus_tag="ACHY09_001902"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFS3884881.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNQ
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3533..6373)
                     /gene="glnE"
                     /locus_tag="ACHY09_001903"
     CDS             complement(3533..6373)
                     /gene="glnE"
                     /locus_tag="ACHY09_001903"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFS3884882.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFLQD
                     SIIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPFDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(6396..7697)
                     /gene="ygiF"
                     /locus_tag="ACHY09_001904"
     CDS             complement(6396..7697)
                     /gene="ygiF"
                     /locus_tag="ACHY09_001904"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFS3884883.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7939..8559
                     /gene="ygiM"
                     /locus_tag="ACHY09_001905"
     CDS             7939..8559
                     /gene="ygiM"
                     /locus_tag="ACHY09_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFS3884884.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8623..9861
                     /gene="cca"
                     /locus_tag="ACHY09_001906"
     CDS             8623..9861
                     /gene="cca"
                     /locus_tag="ACHY09_001906"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFS3884885.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(10049..10870)
                     /gene="bacA"
                     /locus_tag="ACHY09_001907"
     CDS             complement(10049..10870)
                     /gene="bacA"
                     /locus_tag="ACHY09_001907"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS3884886.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10961..11329)
                     /gene="folB"
                     /locus_tag="ACHY09_001908"
     CDS             complement(10961..11329)
                     /gene="folB"
                     /locus_tag="ACHY09_001908"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFS3884887.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11434..12051
                     /gene="plsY"
                     /locus_tag="ACHY09_001909"
     CDS             11434..12051
                     /gene="plsY"
                     /locus_tag="ACHY09_001909"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS3884888.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12064..12996)
                     /gene="ttdR"
                     /locus_tag="ACHY09_001910"
                     /gene_synonym="dan"
     CDS             complement(12064..12996)
                     /gene="ttdR"
                     /locus_tag="ACHY09_001910"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFS3884889.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13203..14114
                     /gene="ttdA"
                     /locus_tag="ACHY09_001911"
     CDS             13203..14114
                     /gene="ttdA"
                     /locus_tag="ACHY09_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFS3884890.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14111..14716
                     /gene="ttdB"
                     /locus_tag="ACHY09_001912"
     CDS             14111..14716
                     /gene="ttdB"
                     /locus_tag="ACHY09_001912"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFS3884891.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14765..16228
                     /gene="ttdT"
                     /locus_tag="ACHY09_001913"
     CDS             14765..16228
                     /gene="ttdT"
                     /locus_tag="ACHY09_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFS3884892.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16271..17284)
                     /gene="tsaD"
                     /locus_tag="ACHY09_001914"
     CDS             complement(16271..17284)
                     /gene="tsaD"
                     /locus_tag="ACHY09_001914"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS3884893.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17522..17737
                     /gene="rpsU"
                     /locus_tag="ACHY09_001915"
     CDS             17522..17737
                     /gene="rpsU"
                     /locus_tag="ACHY09_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS3884894.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17848..19593
                     /gene="dnaG"
                     /locus_tag="ACHY09_001916"
     CDS             17848..19593
                     /gene="dnaG"
                     /locus_tag="ACHY09_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS3884895.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKQAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDNQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19788..21629
                     /gene="rpoD"
                     /locus_tag="ACHY09_001917"
     CDS             19788..21629
                     /gene="rpoD"
                     /locus_tag="ACHY09_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS3884896.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(21707..22213)
                     /gene="mug"
                     /locus_tag="ACHY09_001918"
     CDS             complement(21707..22213)
                     /gene="mug"
                     /locus_tag="ACHY09_001918"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFS3884897.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22338..22413
                     /locus_tag="ACHY09_001919"
     tRNA            22338..22413
                     /locus_tag="ACHY09_001919"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22371..22373,aa:Ile,seq:cat)
     gene            complement(22467..23231)
                     /gene="nfeF"
                     /locus_tag="ACHY09_001920"
                     /gene_synonym="yqjH"
     CDS             complement(22467..23231)
                     /gene="nfeF"
                     /locus_tag="ACHY09_001920"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFS3884898.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISVGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            23520..24143
                     /gene="nfeR"
                     /locus_tag="ACHY09_001921"
                     /gene_synonym="yqjI"
     CDS             23520..24143
                     /gene="nfeR"
                     /locus_tag="ACHY09_001921"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFS3884899.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(24247..25767)
                     /gene="aer"
                     /locus_tag="ACHY09_001922"
     CDS             complement(24247..25767)
                     /gene="aer"
                     /locus_tag="ACHY09_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFS3884900.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLTAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
BASE COUNT         6217 a         7129 c         6624 g         5926 t
ORIGIN      
        1 ctggatgttt accgcgatta ttgccgtatg cattctgttt attattggaa ttattttcgc
       61 caggctctat cgtcgcgctt cggcagagca agcttttgtt cgtactggtt taggtgggca
      121 aaaagtggta atgagcggtg gcgcaatcgt gatgccgatc tttcatgaaa taatccccat
      181 caatatgaat actctgaagc tggaagtcag ccgctcaacc attgatagcc tgattacgaa
      241 agatcgtatg cgcgtcgatg tcgttgtcgc tttctttgtg cgggtaaaac cgtcagtgga
      301 aggtattgcc accgccgccc agacgcttgg gcaacgcacc ctgtcgcctg aagacttacg
      361 tatgttggtt gaagataaat ttgtcgatgc cctccgtgca acagctgcac aaatgaccat
      421 gcatgagtta caggataccc gcgagaactt cgtgcagggc gtacaaaata ccgtagctga
      481 agatctgtcg aaaaacggcc tggaactgga aagcgtttca cttaccaact ttaaccagac
      541 gtcgaaagaa catttcaatc ctaacaacgc ctttgacgcc gaaggtttaa ctaagctgac
      601 gcaggagacg gagcgccgtc gtcgcgaacg taacgaagtt gaacaggatg tagaagttgc
      661 ggtgcgtgag aaaaaccgtg atgcgctttc gcggaagctg gagattgaac agcaagaagc
      721 gtttatgacg cttgagcagg agcagcaggt taaaacccgt accgctgagc agaatgcgaa
      781 aattgcggct tttgaggccg agcgtcgtcg ggaagcagag cagacgcgaa ttctggctga
      841 acgacagatt caggaaacgg aaatcgaccg cgaacaggcc gtccgttcca gaaaagttga
      901 agctgaacgt gaagttcgca ttaaagagat cgaacagcag caggtcaccg aaatcgccaa
      961 ccagacgaaa tcgatcgcga ttgccgccaa atcggaacag cagtcacaag cggaagcgcg
     1021 tgccaacctt gcacttgcag aagcggtaag cgcccagcaa aacgtagaaa ccactcgcca
     1081 gactgccgaa gccgatcgtg ctaaacaagt ggcgttgatc gccgcagcgc aggatgcaga
     1141 aaccaaagcg gttgaactga ccgtgcgggc gaaagcagaa aaagaagccg cagaaatgca
     1201 ggcggcggct atcgttgagt tagccgaagc tacacgtaaa aagggtctgg cggaagcaga
     1261 agcacaacgt gcgctgaacg atgctatcaa cgtactttct gatgaacaaa ccagccttaa
     1321 attcaaactg gcattattgc aggcgctgcc tgcggtaatt gagaaatccg tggaaccaat
     1381 gaagtctatc gacggcatca aaattattca ggtcgatggg ctgaatcgtg gtggcgctac
     1441 gggtgatgca aacacgggta atgtgggggg cggaaacctg gcagaacaag cgttatcagc
     1501 cgcactctct taccgtacac aggcaccgct gattgactcc ttgctcaatg aaattggcgt
     1561 ttcaggcggc tcactggcgg cattgacttc atccttaagc tcaacaactc ccgtcgaaga
     1621 aaaagcagag taaaccctct tctatgaata atccctggcc caaaaagccg gggatttttt
     1681 atatctgcgt tccgctaaaa ggtgcaaatg atcaagctgt tgcagcattt tgcgtgactg
     1741 ctcggggaag gcaaaactgc ctctgggaaa gcgttgcgcg gggtccggcg ctcatcaaca
     1801 atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg ggccttgtat
     1861 aaggaaaggg ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc
     1921 gcagcttatt aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac
     1981 tttaggttga aaaaaaatca gcgtcaatag gcccgcctgc tacaaagcga gatctgtgaa
     2041 ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt ggtcgtcgag
     2101 caaccgtctt caaagttgag caccagcact tcaccaccgt tggcccagac ttctttactc
     2161 ccggcaatct cttctggttt atagtcgccg cctttcacca gtagatctgg caagataccg
     2221 gcgatcaagc gctgcggcgt gtcctcttca aacgacacca cccagtcgac cgcttccagt
     2281 gcgcccagca caatcatacg ctgttcgagc tggttaacgg ggcgggaatc ccctttcagc
     2341 cgtttggtgg aggcatcgct gttcacagcg acaatcaaac ggtcacccag tttacgggca
     2401 tttgccagat aagagacgtg cccagcgtgc aggatgtcaa agacaccgtt ggtcatcacc
     2461 actttttcac cgcgtttacg cgctgccgct acagcaagct tcagttcctc ttcggtcatc
     2521 acaccaaagc cggtatctgc acgtccacgt acagcgtttt ccagctcgat cggcgaaacc
     2581 gtggatgtcc ccagtttgcc gaccaccaca ccagccgccg cattggcaaa gaagcaggct
     2641 tcttccagtg aattacccgc tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc
     2701 gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagtgg cgctttaccc
     2761 ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga gagttcgtaa
     2821 tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca tttaccgaca
     2881 acagcttcaa attccgagag attcggcgtt aacagcgtag cgccgcggta gcgctcaaaa
     2941 tcggtacctt ttggatcaat cagcaccgga acacccgctt tacgcgccag ttggatcatc
     3001 tgctgtacgc ttgccagcgc acctttggcg taatcagaaa gcaccagcgc gccaatcgaa
     3061 ctcagtgcct gattaatccg ctcgtgcagc ggctgcggat caaccccttc gaaaccttct
     3121 tcgaaatcca gacggatcag ttgttggttg cgggaaagta cccgtaattt ggtgatggtc
     3181 ggatgcgtcg gtacagaaac aaagtcgcat ttgacgttga cgtcggccag agatttactg
     3241 agcgcgcgcg ccgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga
     3301 gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat ggtattcact
     3361 ttaaccacgg gcaccggcgc ttccggcgag atacggctgg tgggaccgta ccagtaacga
     3421 tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg cagcgttact
     3481 ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata cttcattctt
     3541 ccaccagcca cttctgccag cttgcccgca ccagttcacg ctctgcggtg aagcaatcct
     3601 ccgacacatg acccggcaat tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg
     3661 tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt tgcgccagta
     3721 gttccagaat gcgcacgttg tctgaccagc gcgttaactt cggtttttcg tgagcgtagc
     3781 gcaacaccag atattgagta ataaattcga tatcggtaat tccgccttca tcggctttga
     3841 tatcaaagcg atcgcgatgt ttattgccga gatgggcacg cattttctcg cgcatttccc
     3901 gcacttctgt ttgcagggtt ttgccttcgc gcggcagcgt cataatctcc cggcgtactg
     3961 cgtcaaagtg cgcggtgagc tgcggatcgc cgtacactac acgcgcacgc accagcgcct
     4021 gatgctccca cgtccaggcc tcgtttttct gataatcagc aaatgcttct gcggatgtca
     4081 ccagcattcc cgccgccccg gacggacgca gtcgtgcatc cacttcatac aaaatgccgg
     4141 aagaggttcg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga taaaactgtc
     4201 gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca tggaggaaga
     4261 tcaggtcaag atcggaactg tagcctaact cccagccacc cagcttgccg tagccgacca
     4321 ccgcaaaacc acgcccttcg cgttcgttca ggtgatttgg cttaccgtag cgggcaacca
     4381 tttgaaccca cgcctgctga acgacggcat caatcatggc ttctgccagc caggttaagt
     4441 gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca atgcgtaaca
     4501 gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca tcttccggca
     4561 cgcgcagcaa atactggcgc agttcatcac ggtaggcatc cgtcgccgtt ggttggtaga
     4621 gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc tggctggcaa
     4681 tcatcggcga tgcagcacac agagaaatca aatgtttaag cgccgcaggg aattcactaa
     4741 gcagctcaag ataagtggtg cgagtaacaa tccccaccag caaggcggta atgcgcgaca
     4801 gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc atcagatggt
     4861 cgagcacctg acgtcctcgc ggcccgatgg tgcgcttatc cagctctttg cggaaatcag
     4921 caatcagcat tagcacctgt ttgcgatcat cctcgctaag atgcgccagc actggcgtgg
     4981 tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac agcgactctt
     5041 cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc acattggcca
     5101 tatgtgcggt cagcaccccg gtcaattgcg gccagtcagc aaagtccatc gcccacgcca
     5161 gccgcgcacg gttaagctca tcaaaaggaa gcgtctgggt ttgttcgtcg ttaatgcttt
     5221 gcagcaggtt ttccagacgc cgcaggaaca gatacgccac tcgcaattgt tcagcatcgt
     5281 tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa gagcgcgatt
     5341 gcagcgacgg ttcgcgcccg ccgcgaatga gctggaacac ctggacgata aattcaattt
     5401 cgcgaatgcc gcctgcgcca agtttgatgt tgtcggtcaa accgcgtcga cgcacttcac
     5461 gggcaatcat tcctttcatg ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac
     5521 ggaaaacaaa cggacgcagc atcgcacgca actcgttagc atagacgccg tcgctatccc
     5581 ccataatccg cgccttgacc atcgcgtaac gctcccagtc gcgcccctgc tcctggtaat
     5641 aatcttccag cgccgcaaaa ctcagcacca gcgggccact ttcaccaaac ggacgcagcc
     5701 gcatatccac gcgatagacg aaaccatcct gcgttggttg atccagcact ttaatcagcc
     5761 gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg ccctgggtac
     5821 aaccatgttc cggccaggca aaaatcagat cgatatctga ggagaaattc agttccccac
     5881 cgcccagctt acccatgcct aaaatcagca gcggttgcgc ttcgccctgc gcattgcacg
     5941 gcgttcccca ttcgcggcag caggcgtcat acagccagtc acgcgccgca acaatcagcg
     6001 tctccgccag atggctgagc tgctgcaata tgctctcttc agtaaccagt gccagcgttt
     6061 gcgcccaggc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg cgcattaacc
     6121 cggcttcgtc actcacatta ctgagcgcct cctgcaacca tgctgcgtaa tgctgccatt
     6181 cgtcggcctg cggcggttgg ctttccagtt ccgtcagcca ctctggatgc gcaattatgc
     6241 tgtcctgcag aaaatcactg aaagtaagta ctgacttcgc ctgtgcgcta agtgattcct
     6301 cggctaaagg ctctggcagc cgctcaacaa cggtctgcca gtactgctgt aacggtgaag
     6361 agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct gtgcaaccag
     6421 aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg ttgcccggtc
     6481 gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac gacaggatcg
     6541 tagtaacccg ccagcaacag gattgagtca atatcacgcg tcaggcgtgg caactggtca
     6601 cggtagcgat cgcccaacgg ctggcagaaa acgcttttca gttcagcggc atggcgggaa
     6661 agatggatat cggcaaagcg tttgaaggaa tcgctcattt tactctgcgc tttggcatct
     6721 aaaaacggct gccaggcttt gcttaccagc cattcggtca atgccagctt cgccattgcc
     6781 gtttcggtag agtagaccgc cgtcacggca gaaaccgctg aagcgatggt cgcttcgcat
     6841 tgagtcaata aatcacgtaa gtgagtgctc gctttacgcg gcacaatgcc accgaacagc
     6901 atcagcgtat gacggaccag gccaatggct gccagcacct gctctttcgc cgcatcattg
     6961 ccgcgtaccc atagttcttc atgatactgc cattgcgcta acgccagctc gagcgccgct
     7021 tccagcccct gttccacatc agcttttgcc gcaacatgta aaatggtggt cggtttgatt
     7081 tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct cagactgccc
     7141 tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg
     7201 ccgctaagca gttccagttc cagctcgcag ataggttcag caaattcacc cgctttcact
     7261 tccccctggt cgagggcgat ttcaatttgg ctaccatcaa cctctaccag ccacttttcg
     7321 cgataaaaat cggtgctaaa cagcggctgc acgcgggaag cgagatcggt aggcaattcg
     7381 ccgttcggcc agacttccgt cggtaactgc gccaggtcga gcgtcggttc gctcaacgcc
     7441 acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc
     7501 atctcatagc gaccgttttc gccacgaata cgcaagccca tgtcgtgccc acgcagccag
     7561 ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg ctcgccgccc
     7621 agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat aaactttaat
     7681 tcgatttcct gagccatagc ctttacttat cggttatgtc acataagtga cgatgaacgg
     7741 cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt gccatgcaac
     7801 gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca gaggattccg
     7861 attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc cactttccat
     7921 gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt actcgcactt
     7981 agcgccactg ccgtctcaca cgctgaagaa acgcgctatg tttctgatga actgaatacc
     8041 tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa cgccggcgag
     8101 gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa agacagctct
     8161 ggccgtaccg cctggatccc gttgaaacaa cttagcacag agccaagcct gcgctcccgt
     8221 gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa tatcgataac
     8281 acctggaacc aacgcaccgc agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc
     8341 aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc gcagaaaaag
     8401 gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat gcagtggttt
     8461 atgtatggcg gtggcgtgct ggggcttggc ttgctgcttg gtctggtact gccgcacctg
     8521 atcccaagcc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt cgttgcactg
     8581 tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat ttatctggtc
     8641 ggtggtgctg ttcgggatgc attgttaggg ctaccggtca aagacagaga ttgggtggtg
     8701 gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg ccgcgatttt
     8761 cctgtgtttc tgcatccgca aacgcatgaa gagtatgcac tggcacgtac cgaacggaaa
     8821 tctggttccg gttacaccgg ttttacctgc tatgccgcac cggatgtcac gctggaagat
     8881 gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa cggcgagatt
     8941 atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc
     9001 gcttttggcg aagatccgtt acgcgtgttg cgcgtagcgc gttttgctgc gcgttatgcc
     9061 cacctcagtt tccgtattgc cgatgaaact ctggcgctga tgcgcgagat gacccatgcg
     9121 ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaaa cgccctcacc
     9181 acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggcgcact gcgcgtttta
     9241 ttcccggaaa ttgacgccct gtttggcgtt ccggcccctg ccaggtggca tccggaaatc
     9301 gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag tccgcaggtc
     9361 gatgtccgtt tcgcaacgtt atgccacgat ctcggtaaag gactgacgcc accagaactt
     9421 tggccgcgtc atcatggtca tggcccggcg ggtgtgaagt tagtggaaca actatgccag
     9481 cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga gtttcacgat
     9541 ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaactatt tgattccatc
     9601 gacgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga ggctgatgtg
     9661 cgcggcagaa ccggttttga atcagccgac tacccgcaag gccgctggtt gcgcgaagcc
     9721 tgggaagtgg cacagtcagt gccgacaaaa gccgtcgttg aagcgggatt taaaggtgtg
     9781 gagattcgcg aggagctgac ccgacggcgg attgcggcgg tggctggctg gaaggaacag
     9841 cgttgcccaa aacctgagtg agtgttggat attttgccgg atgcgacgta agcgtcttat
     9901 ccggctacat gtcaacgaca gttgtaggcc tgataagacg cgcgagcgtc gcatcaggcg
     9961 ttgattgccg gatgcggcgt gaacgcctta tccggcctac gcaatctgat aagacgcgtc
    10021 agcatcatat ccgacactgt tcttcacatc aaaagaacac aacgtacacc gcagccgcca
    10081 caataaagcg ataaatggcg aacgggataa acgaaatgcg cttaatcaat tgcaggaagg
    10141 ttttaatcgc tatcagcgcc accacaaaag cggtgataaa cccaacggca aacatcggga
    10201 tatcgccggt agtcaggaag ccccagcttt tgtagagatc gagcgccgtt gcgcccatca
    10261 tcatcggcac cgccagcagg aacgagaact cggaagcagc gtaacggctc acccccatca
    10321 gcatcccacc tgaaatggtc gccccggaac gggagaaacc cggccacagc gccagacact
    10381 ggaaacagcc aatcataaac gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg
    10441 gctctttcgg cttcaggcat tcggcggcaa tcagcaacag accgccaacg accagtgcat
    10501 acatcacatt tatcgggtta aacaatgatt taatcgtgtc gtggaacaac agccccagta
    10561 ccaccgccgg aatcatcccc agcaaaatgt ggatcagcgt taaacggcct ttgctttctc
    10621 cttcgtgctg caacgggcga ccaaagtgaa taccaataag gccaaacaga cgtcgccaga
    10681 acatgacaac cactgccaga attgatccta actggatcac gacttcaaag gttttcgccg
    10741 tgtcgccctc aaaccccaac aagtgaccga caataatcat atggcccgtg ctggataccg
    10801 gcagaaattc tgtcaatcct tcgaccacac ccaatattgc cgctatcagc agcgagtgca
    10861 tatcgctcat caataaaccc ctaaattata aaaatgtaac gcttgtccga actactgcag
    10921 tatgaccagg ttataaccgt ttggtttaac agctgtaaaa ttaattattt tctttcagat
    10981 tattgccacg ctcaatgatt acgccaacat tcgccgcccg cgccactgcg cctggcttgc
    11041 tgagtttgat acgcacccac ggcgagttga agcgtgctaa cagcaactcc gccacctctt
    11101 cagccacacg ttccaccagc gcaaaacgcg ccccctcgac gtggctgact accgtttctg
    11161 caatgtcagc gtaactgagg caatccgcca catcatcact tttcgccgct ttacggttat
    11221 cccacgccat ttcgatatcg aacactaact tctgttcgat ggtctgttcc cagtcgtaaa
    11281 caccaatagt ggtgattacc gaaagttgct ctataaatac aatatccatc acgtcctgcc
    11341 tgctttttgg ctaaccggat accacttccg gcgaaatgtg cgtattatcc acagattcat
    11401 cgttgaacac gaattttcaa aacggaacag cttatgagtg caatcgcgcc tggaatgatc
    11461 ctcatcgcgt acctctgcgg ctccatttcc agtgccattc tggtttgccg cttgtgtggg
    11521 ctgcccgatc cgcgaaccag cggctccggc aatccaggcg caaccaatgt gttacgtatc
    11581 ggtggcaagg gagcagccgt agcagtactg attttcgacg ttctgaaagg aatgttgccc
    11641 gtctggggcg cgtatgaatt aggtgtcagc cccttctggc taggcttaat tgccatcgcc
    11701 gcctgtcttg gacacatctg gcccgttttc ttcggattta aaggaggaaa aggcgttgct
    11761 accgcttttg gtgccatcgc acccattggc tgggatctca ccggagtaat ggcgggaacc
    11821 tggttactga ccgtgctatt gagcggatat tcgtcgctgg gggcgattgt cagtgcactg
    11881 attgctccgt tttatgtctg gtggtttaag ccacaattca ccttcccggt ttcgatgctc
    11941 tcttgcctga tcctgctgcg ccatcatgac aacatccagc gtctgtggcg tcgtcaggag
    12001 acaaaaatct ggacgaaatt caaaagaaag cgcgaaaagg atcccgagtg atttctggtg
    12061 gagctacata acctgatagc cttcgtcggg cttgcccagc cgttgctggc accatgccgc
    12121 cagaaattcc acgcagacac gtaatttcat gctgcgatag agcggctccc ggtaaacagc
    12181 ccagatattg gcgctttgtg catactccgg caatactcgc accagtttgc cactctccag
    12241 aaacggcaac acatcccact cggaacgcaa cataatccct ttgccctcca gcgcccattg
    12301 caacactatc tcgccgctat tggaggaaag atgcccgctt accttcaccg attttttctc
    12361 ctgaccgttc cccaactccc atattccgtg ggtcatatcg cgctctttgg tcaccaggca
    12421 gtcatgacga cttaattcct gtaaggattg tggctgcgga tatttttgca gatactcagg
    12481 tgctgcgcat aatattcttt tattttttgt caaaagatgc gcaatataat aatcaggaat
    12541 tgcgtcatta atacgaatat ccagatcaat attatcctgc actaaatcaa tttgccgatc
    12601 gaacaattca aaatgcacct gtaactcagg ataattgcgc atcagttcgg taatggctgg
    12661 agcaatatgg ctgcgcccaa aaccaaaact gcaaccaatg cggatcatcc cctccgggcg
    12721 cgttttgatt tgcgtgacgt catcgaccag tcgctgatat tgggtgagga tttccagcgc
    12781 atgttcgtaa caacgttggc cactttcagt cagcgccacg ccgcgagcgg agcggttcag
    12841 cagagttgtt gccagcgtat tttcaaggat ttggatccgc ttggtgacaa atgcaggcgt
    12901 ttgcccaagc gtcgccgctg cagcactaaa actcccgcta tggacgattt ccaccagtac
    12961 ctgtaagtct ttggctaaag gccagctatt tagcattgtt aaatatccgc agttttgata
    13021 aacgcagtgt atttcgcaaa acattaccat tccctgctct ggcgcaatat tcgttcttat
    13081 tttcgcgttg attcacgaac tgttaattac atattcacgg tggcaaaaaa tataaaacca
    13141 catttttagt ggtagtttgt ggcggtgaat ttttccagac aaatacataa actggagttg
    13201 ccatgatgag cgaaagtaat aagcaacagg cagtgaataa gttgacggag attgtcgcta
    13261 actttaccgc catgatttct acccgaatgc ctgatgacgt ggtggataaa ctaaaacagc
    13321 taaaggatgc cgaaacatcg tcgatgggaa aaatcatcta ccacacgatg ttcgataaca
    13381 tgcaaaaagc gatcgacctg aatcgtcctg cctgtcagga caccggcgaa atcatgtttt
    13441 ttgttaaggt cggttcccgt ttcccactgc ttggcgagct gcaaagcatc ctcaaacaag
    13501 ccgtggaaga ggcaaccgtc aaagcgccac tgcgtcacaa tgcggtagaa atttttgacg
    13561 aagtgaacac cggcaaaaat accggcagcg gcgtaccgtg ggtcacctgg gatatcgtcc
    13621 ccgacggtga cgatgcggaa atcgaagttt acatggcagg cggcggctgc acactacctg
    13681 gtcgttcgaa agtgttaatg ccgtcggaag gatacgaagg cgtggtgaaa ttcgtcttcg
    13741 aaaatatctc caccctcgcc gtaaacgcct gtccaccggt actggtaggc gtgggcatcg
    13801 ccacctcggt ggaaaccgcc gctgtgctct cacgtaaagc cattttacgc ccaattggca
    13861 gccgccaccc caatccgaaa gcggcagagc tggagctacg cctggaagaa ggactcaacc
    13921 gtctggggat tggtccacaa gggctgactg gcaacagttc agtgatgggc gtacatatcg
    13981 aatctgccgc ccgccatccg tcaaccatcg gcgttgctgt ttctacaggc tgctgggcgc
    14041 atcgtcgcgg cacactgctg gttcatgccg atctcacctt tgaaaatctg tctcacaccc
    14101 ggagcgcgtt atgaaaaaga tcctgacaac cccgatcaaa gctgaagatc tgcaagatat
    14161 tcgcgtcggc gatgtgatct acctgaccgg tacgctggtg acctgccgtg acgtttgtca
    14221 ccgtcgcttg atcgaactca aacgtccgat cccttacgat ctcaacggca aagcgatttt
    14281 ccacgctggc cccatcgtgc gcaaaaacgg cgacaaatgg gagatggtat ccgtcggccc
    14341 gacaaccagt atgcgtatgg aaagttttga acgtgaattt atcgaacaga ccggcgtgaa
    14401 actggtggtc ggcaaaggcg gaatggggcc gctgaccgaa gaaggctgcc agaaattcaa
    14461 ggcgctgcat gtgattttcc cggcaggctg cgcagtactg gcggcaaccc aggtggaaga
    14521 gattgaagaa gtgcactgga cagaactcgg gatgccggag tcgctgtggg tctgccgggt
    14581 caaagagttc ggtccgctga ttgtttctat tgatacccac ggcaacaacc tgatagccga
    14641 aaacaaaaag ctgttcgccg aacgccgcga tcccatcgtg gaagagattt gcgagcacgt
    14701 ccactacatc aaataaccct cccggagagg ctcaccccct ctcctttttc gcaggcataa
    14761 cacgatgaaa ccttccactg aatggtggcg ataccttgcg ccgctggcgg tcatcgccat
    14821 tattgctcta attccggttc ccgcagggct ggagagtcat acctggctct actttgccgt
    14881 ttttactggc gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat
    14941 ggtgggtatc tccattatcg ccattctctc gccgtggctg ctgtttagcc cggagcagct
    15001 cgcccagcca ggttttaagt tcaccgccaa atccctctcc tgggcggttt ctggtttttc
    15061 taattcggtt atctggctga ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac
    15121 cgggcttgga cgccgcattg cgctgattct ggtgaaaaag atgggacatc gcacgctgtt
    15181 tctcggctat gcggtgatgt tctccgagct gatcctggca cctgtaacac cgtccaactc
    15241 ggcgcgcggt gcggggatta tctatcccat catccgtaac ctgccaccgc tctatcaatc
    15301 acaaccaaac gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt
    15361 tgccgactgc gtgaccagcg ccattttcct gacggcgatg gcacctaact tgctgttaat
    15421 tggcctgatg aaaagcgcat ctcacgccac actgagttgg ggcgactggt tcctcgggat
    15481 gttgccgctc agtattttac tggttctgct ggttccctgg ctggcttacg tgctgtaccc
    15541 gccggtactg aagtctggtg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat
    15601 gggcccgctc tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct
    15661 gtggattttc ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcgct
    15721 gatgctgctt ctgcgcatta tcagttggga cgacattgtc agtaataaag ccgcgtggaa
    15781 cgttttcttc tggctggcct cgcttatcac cctcgctacc ggactcaaca acaccggttt
    15841 tattagctgg tttggcaaac tgttagcagg cagcttaagc ggttattcgc caacgatagt
    15901 gatggtggcg ttgattgtgg tgttttatct actgcgctac tttttcgcca gcgccacggc
    15961 gtatacctcc gctctcgcgc cgatgatgat tgccgccgcg ctggcgatgc cggaaatccc
    16021 gctgccggtg ttctgcctga tggttggcgc ggcaattggt ctggggagca ttcttacgcc
    16081 atacgccacc ggacccagtc cgatttacta cggtagtggt tatctgccaa cggtggatta
    16141 ctggcgactg ggggcaattt ttgggctgat attcctcgtg ttgctggtga ttaccggctt
    16201 actgtggatg cccgtggtgt tgctttaagt gaggacggtg ccggaagtga ctctccggca
    16261 cctaagagtt ttacgcggcc ggtaactctg ccagcggcca gcgcggacgc acgctaacgc
    16321 caagatccgc cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc
    16381 cgttatcagt acaaaattcc ggacgcgcgt agaacacttc gccacggcgt tttttcatca
    16441 tttccgccag cttcgcccgt aacgtgcggt tagcactcac gccacctgcc attaccagtc
    16501 gcttaaagcc cgtctgatcc agcgctcgct tacacttaat catcaacgta tcgaccaccg
    16561 catcttcaaa ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg ttatcacgaa
    16621 tggtatttgc cgcgaaggtt ttcagaccag aaaagctgaa atccagccct ggacggtcgg
    16681 tcatcgggcg cgggaagaca aagcgcccgg ctgtaccctg agccgccatt ttcgacagta
    16741 atggcccgcc aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccag
    16801 ccgcatcatc gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta
    16861 actgcgtatg accgccggaa accagcagcg ccacaaacgg aaattccggt gggttatctt
    16921 ccagcatcgg cgctaacaga tgcccttcca tatggtgtac agggatcgcc ggaacgttcc
    16981 aggcaaacgc cagagaacgc cccacggtcg cgccaaccag cagtgcgccg actaatccag
    17041 ggcctgcggt ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg
    17101 ccgcctggat caacggtacg gttttacgca catgatcgcg ggaggccagc tcaggcacta
    17161 cgccgccgta gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt
    17221 tttcatcgtc gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac
    17281 gcatgacttg ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt
    17341 aaaactttgt tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc
    17401 tttcaaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca
    17461 ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca
    17521 catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa
    17581 gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa
    17641 accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact
    17701 ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg
    17761 atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg
    17821 tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat
    17881 gatctgctgg cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag
    17941 cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc
    18001 gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc
    18061 gacttcctga tgaactacga caagcttgag ttcgtcgaaa cggtcgaaga gctggcggca
    18121 atgcacaatc ttgaagtgcc atttgaagca ggtagcggcc ccagccagat cgagcgccat
    18181 caacgacaaa cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta
    18241 cagcaacctg ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag
    18301 gttatcgccc gctttgcgat tggttttgcc ccccctggct gggacaacgt cctgaagcgg
    18361 tttggcggca atccagaaaa tcgccagtca ttgattgatg cgggcatgtt ggtcactaac
    18421 gatcaggggc gcagttacga ccgtttccgc gagcgggtga tgttccccat tcgcgataaa
    18481 cgcggtcggg tgattggttt tggcgggcgt gtgctgggca acgatacacc caaatacctg
    18541 aactcgccgg aaacggacat tttccataaa ggccgccagc tttacggtct ttatgaagcg
    18601 cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg
    18661 gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc
    18721 gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac
    18781 cgtgcaggcc gcgatgccgc ctggcgagcg ctggaaacgg cgctgcctta catgacagac
    18841 ggtcgtcagc tacgctttat gtttttgcct gatggcgaag accctgacac gctggtacga
    18901 aaagaaggta aacaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt
    18961 ctgtttaaca gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg
    19021 agtacgctgg cactgccact gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt
    19081 cgtcaggaat taggcaacaa attaggcata cttgatgaca accagcttga acgattaatg
    19141 ccaaaagcgg cagagagcgg cgtttctcgc cctgttccac agctaaaacg cacgaccatg
    19201 cgtatactta tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt
    19261 gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact
    19321 tgtctctctc agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat
    19381 aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct
    19441 gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag
    19501 gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc
    19561 tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga
    19621 tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt
    19681 aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg
    19741 tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc
    19801 cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg
    19861 ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca
    19921 tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc
    19981 tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt
    20041 ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa
    20101 tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag
    20161 acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc
    20221 tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct
    20281 ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc
    20341 tttcccagga agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca
    20401 gcgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactgc
    20461 gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg
    20521 ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc
    20581 agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac
    20641 gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc
    20701 tgtttaccgg caacgaaacc agcgatacct ggttcaacgc ggcaattgca atgaacaagc
    20761 cgtggtcgga aaaactgcac gatgtctctg aagaagtgca tcgcgccctg cagaaactgc
    20821 agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta
    20881 tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac
    20941 gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga
    21001 ttcaggaagg caacatcggt ctgatgaaag cggttgataa attcgaatac cgccgtggtt
    21061 acaagttctc cacctacgca acctggtgga tccgtcaggc gatcacccgc tctatcgcgg
    21121 atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc
    21181 gtatttctcg ccagatgctg caagagatgg gccgtgaacc aacgccggaa gaactggctg
    21241 aacgtatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa
    21301 tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg
    21361 ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc
    21421 acgacgtgct ggctggcctg accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta
    21481 tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg
    21541 aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg
    21601 aagtgctgcg tagcttcctg gacgattaat cggtaggccg gatcaggtgt tacgccgcac
    21661 ccggcactag tccctccgca caaacgccac cttttcggtg gcgtttttat cgcccacgca
    21721 ccaccagtgc ctggtccagc tcgcgatacg cttcaaccag tttctccagt gaaacgcgac
    21781 ttaaaccgct gggatttggc agcacccaaa tctgcgtcga accaatggtg agagtttgtt
    21841 tcccccactg tgcaccgcgc tggctgaatc cctgttcata tgcttgtttg cccagaatcg
    21901 ccaacgcctg cggctgataa tcttcaattt tttcaatcag cttacgcccg cctgcgtgta
    21961 gctcctgctt tgaaacttca ttggcttgca ccgttggacg gtctaccagt ttggtgacgc
    22021 cacaacgata atccagcaga tgctgtgcct cctgcggctt caactgacgg tcggtaaacc
    22081 cggcctgata tatcaccttc cagaagcgat ttgccggatg agcaaaggga aaaccagtcc
    22141 cggcggatga aagcccaggg ttgataccgc aaaacacgac ccgtaaccct ggagccaaaa
    22201 tatcctcaac catatttact cctttctaac atcaccagga aaagtataac ggattgaaaa
    22261 tacattgttt ataaaaacag caggcgcgcg gtaatggctg gattgcgaca cggagttact
    22321 ttataatccg ctaccatggc cccttagctc agtggttaga gcaggcgact cataatcgct
    22381 tggtcgctgg ttcaagtcca gcaggggcca ccagatatag caaaggctga cgagaaatcg
    22441 tcagcctttt tctttttata tatcagttac tttgcgtgcc agtaagccgc tgcacgtacc
    22501 cgctgtgggt catactgttc cgcttcaaag cggcggctta aattcttaac gactttgcct
    22561 tcgccggtta tccagatgaa gtaatcatcg gcagggattt gcatctgcgc cagacgcgca
    22621 tctaccgcct gctcatcatg cgccagccat tcgatattaa aaccatcaag gtgcgcgaga
    22681 taatcctgac aggcgttatc ccgcacgcta accagcgcac taacttgcgg tttaacggca
    22741 agtttgctca acgtttccag gcggcggcgc agtgcgggca ttccggattc atcgcagaca
    22801 tacagctgat acgcgtaatc ctctggcacc accagcgaac cgcgcggacc tgccaccgtg
    22861 agtttatcgc ccggttgcgc ctgcattgcc cagctgctgg cgacgccacc ttcgtgaata
    22921 aagaaatcaa tcgccagttc atggcgtagt tcgtcataca gcggcgtata gtcacgcgac
    22981 ggtgggcgtg gtccttccgg ccagacgatg ccctcttccg ttaccgttgg cggcacaaag
    23041 tgagcatcag attgaggaaa gaagagtttg ctgtgatcgt caaagccgcg cgatgtaaaa
    23101 ccgtccagcg cctcgccgcc aaggacaatg cgctgaaaac cgacgctaat acgctcaacg
    23161 cgtagcacag tcaattcacg gaagcgcaga tcattgcgaa cgcgctgtgg gtagcggggg
    23221 gtgttattca ttgttatcgc cttcatgatg gtaatcagat atatctaaat aaaatttcgc
    23281 aaatgataat gattgttaat catgataaat gcaagcgatt tgtagaactg atatgtctat
    23341 agtctgataa gacgaaccgc ctcttctcag gcatcattac tcaacgccgg atgcggcgtg
    23401 aacgccttat ccggcctacg tgtgagatga gtccacgttt aaaaatcatt attgtacttg
    23461 cagtaatgct catatcagat ataaattaga tatatctaat taaacaaaag gaggctgata
    23521 tgagccatca tcacgaaggg tgctgtaaac atgaaggcca gccacgccat gagggctgct
    23581 gcaaaggtga gaagtcagaa cacgagcact gcggacacgg tcaccagcat gaacacggtc
    23641 aatgctgcgg tggtcgccac ggtcgcggcg gcggtcgtcg acaacgtttc tttggtcacg
    23701 gtgaattgcg tctggtgatt ctggatattc tctcgcgcga tgacagccac ggttacgagt
    23761 tgattaaagc gattgaaaat ctgacccagg ggaattacac cccaagcccg ggcgtcatct
    23821 acccaacgct ggattttctg caggagcagt cgctgattac cattcgcgaa gaggaaggtg
    23881 gtaagaagca gattgcgctg accgaacaag gcgcgcagtg gctggaagaa aaccgcgaac
    23941 aggtagagat gattgaagaa cgcatcaaag cgcgttgcgt tggcgctgcg ctgcgccaga
    24001 acccgcaaat gaagcgggcg ctggataatt ttaaagcggt actggattta cgcgtcaacc
    24061 agagcgatat cactgatgca caaataaaaa agatcattgc ggtgatcgac cgcgccgctt
    24121 ttgatattac gcaactggat taatcaccga ttgccggatg tggcgtgccc tacccccgta
    24181 ggcctgataa gatgcgccag catcgcatca ggcattgtgc gccaaccgcc ggatgcggca
    24241 tatcgattaa tgcagcaccg tcaccgcgtc ttccagtcgg ctggcgcggt gtttcaccat
    24301 cgccgacacc tgcgcactct cttccactaa ctcggcgttt ttctgggtga tcaggttaag
    24361 ctcatccact gcacgggtca ggctggaaag cccatcggcc tgttccagcg ttgaatggct
    24421 aatctgagct atcaactggg tgacgttttt tacctgtgcc acaatatctt ccatcgtccg
    24481 tccggcggcg tgtacctgct gcgaaccgga ttgcacctta tcagcactgg catcaatcag
    24541 cttgcgaata tcgttggcag cattggcgct gcggctagct aaatgacgca cttcccccgc
    24601 caccactgca aaacctttgc cctgttcgcc ggcacgcgcc gcttccaccg ccgcattcag
    24661 ggccagaata ttggtctgaa acgcaatatc gttaatcagc gaagtaatgg tgccaatgcg
    24721 ctgggtactg tcggcaatat cgtccattgt cttgatcacc gtagtcatcg cctccccacc
    24781 ctgcactgcc gcattactgg cagtgataga aagtttatcg gcagccgacg ccgtggcaga
    24841 gttctgtttc accgacgccg ccatttggtt catggtcgcc accgtttgct gaacgttatc
    24901 aactgtctgc tgggtatgtt cgttcagttc atcggtgcct ttcgccagcg tctcactgcc
    24961 gtttctgaca ctggacacct ggcttgagac atcgttaatt agccaacggc acatcaggcc
    25021 aagctgccct accgcgcgta atgtcagccc cagctcatcg ctgcgattca gatgctcaac
    25081 actattacgc tctccggtcg ccaccttcag tgcctgacgg gcgacatttt ctatcgggcg
    25141 gacaatctgc cattcaaaac aagcgcttgc caataatacc actaacgcac agaggatata
    25201 cgtcaccacc ggggcagcaa caaaccatag catggccgtc agcaagataa acatcagggt
    25261 catcacgcca cgcgcccgcc agcgaagcgg taatgaaggc agtttaccca gccatccttt
    25321 acgcaccacc aggcctttat gaatacgctt actggtacgt ccggcgttca acgctttgta
    25381 cagcggctcc accgccgcga tctcttcatc cgtcgcccgg gtacgaatcg acatatagcc
    25441 actgattttt ccctcgcgca ccatcggtac cgcattggcc cgcacccaat aatggtcgcc
    25501 atttttgcgg cgatttttca cgatgccgct ccagggctcc ccttttttca gggtgaacca
    25561 catatccgca aacgccgctt ttggcatatc cgggtgacgc accatgttgt gcggttgacc
    25621 ttgtaactct tgcaaggtat agccgctcac ctgcacaaaa gtgtcattag catgagtgat
    25681 atagctttgc agatcggtag tggacatcag agtggtatcg tccgccagcg gggtattttg
    25741 ctgggtgaca tacggatgag aagacatgtt cgcgtcctgt gcaggttata tggttgttaa
    25801 cttcttgtca gagtttatgt cggccccgct gcggttatct ttaactgatt aatttgattt
    25861 agatcgcaat ttgcgattaa agcataaatc taatcc
//
LOCUS       DAZUCC010000054        25715 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZUCC010000054 DAZUCC010000000
VERSION     DAZUCC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.054"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(212..1861)
                     /gene="pgi"
                     /locus_tag="ACHY09_001923"
     CDS             complement(212..1861)
                     /gene="pgi"
                     /locus_tag="ACHY09_001923"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS3884901.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2098..2294
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2386..3735
                     /gene="lysC"
                     /locus_tag="ACHY09_001924"
     CDS             2386..3735
                     /gene="lysC"
                     /locus_tag="ACHY09_001924"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFS3884902.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3988..4260
                     /locus_tag="ACHY09_001925"
     CDS             3988..4260
                     /locus_tag="ACHY09_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFS3884903.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4261..5133)
                     /gene="rluF"
                     /locus_tag="ACHY09_001926"
     CDS             complement(4261..5133)
                     /gene="rluF"
                     /locus_tag="ACHY09_001926"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFS3884904.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5481..6428
                     /locus_tag="ACHY09_001927"
     CDS             5481..6428
                     /locus_tag="ACHY09_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFS3884905.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVNALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6498..7304
                     /locus_tag="ACHY09_001928"
     CDS             6498..7304
                     /locus_tag="ACHY09_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3884906.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTRLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVTDFVCYLLSQRASYITGVTTNIAGGKTRG"
     gene            7314..7721
                     /locus_tag="ACHY09_001929"
     CDS             7314..7721
                     /locus_tag="ACHY09_001929"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFS3884907.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     HSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7721..8215
                     /locus_tag="ACHY09_001930"
     CDS             7721..8215
                     /locus_tag="ACHY09_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS3884908.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQHIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8281..9078
                     /locus_tag="ACHY09_001931"
     CDS             8281..9078
                     /locus_tag="ACHY09_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS3884909.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9089..9913
                     /locus_tag="ACHY09_001932"
     CDS             9089..9913
                     /locus_tag="ACHY09_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS3884910.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLLVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9965..11194
                     /gene="sorE"
                     /locus_tag="ACHY09_001933"
     CDS             9965..11194
                     /gene="sorE"
                     /locus_tag="ACHY09_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFS3884911.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11576..12265
                     /gene="pepE"
                     /locus_tag="ACHY09_001934"
     CDS             11576..12265
                     /gene="pepE"
                     /locus_tag="ACHY09_001934"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFS3884912.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12355..13986)
                     /locus_tag="ACHY09_001935"
     CDS             complement(12355..13986)
                     /locus_tag="ACHY09_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFS3884913.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14206..17889)
                     /gene="metH"
                     /locus_tag="ACHY09_001936"
     CDS             complement(14206..17889)
                     /gene="metH"
                     /locus_tag="ACHY09_001936"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS3884914.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMEFLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLSGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18089..18913
                     /gene="iclR"
                     /locus_tag="ACHY09_001937"
     CDS             18089..18913
                     /gene="iclR"
                     /locus_tag="ACHY09_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFS3884915.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            complement(18930..20654)
                     /gene="aceK"
                     /locus_tag="ACHY09_001938"
     CDS             complement(18930..20654)
                     /gene="aceK"
                     /locus_tag="ACHY09_001938"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFS3884916.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGNLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRK
                     QRFSVRFV"
     gene            complement(20870..22174)
                     /gene="aceA"
                     /locus_tag="ACHY09_001939"
     CDS             complement(20870..22174)
                     /gene="aceA"
                     /locus_tag="ACHY09_001939"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFS3884917.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(22204..23805)
                     /gene="aceB"
                     /locus_tag="ACHY09_001940"
     CDS             complement(22204..23805)
                     /gene="aceB"
                     /locus_tag="ACHY09_001940"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFS3884918.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKHILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(24074..25003)
                     /gene="metA"
                     /locus_tag="ACHY09_001941"
     CDS             complement(24074..25003)
                     /gene="metA"
                     /locus_tag="ACHY09_001941"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFS3884919.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            25160..25603
                     /locus_tag="ACHY09_001942"
     CDS             25160..25603
                     /locus_tag="ACHY09_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS3884920.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAAYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         6071 a         6712 c         6739 g         6193 t
ORIGIN      
        1 cagacaaggg gatttatctg ataaaaaatg ccgacataac gtcggcattg tttttaaggc
       61 tatttaatca atcgtactat gtattaattg ccgaatacga tgtacatatc ggcatcgacc
      121 tgtaggcctg ataagacgcg accgcgtcgc atcaggcatc ggttgccgga tgcggcgtga
      181 acgccttatc cggcctacat atcgacaatg attaaccgcg ccacgcttta tagcggttaa
      241 tcagaccatt ggtcgagcta tcgtggctgc tgatttcttt atcatctttc agctctggca
      301 gaatacggtt cgccagctgt ttacccagct ccacgcccca ctggtcgaag gtgaagatgt
      361 tcagaatcac gccctgagta aagattttgt gctcatacag cgcaatcaat gcacccaggc
      421 tgaacggagt gatttcacgc agcaggatgg agttggtcgg gcggttacct tcgaacactt
      481 tgaacggcac cacgtagtca agcgttgccg gatctttacc ctgatcacga tattcctgct
      541 caaccacttc gcgggattta ccaaacgcca gcgcttcggt ctgagcaaag aagttagaca
      601 gcagtttctg gtgatgatca gagagcgggt tatgggtgat agccggagcg atgaaatcgc
      661 acggtaccat tttggttccc tggtggatca gctgatagaa cgcgtgctga ccgttcgtgc
      721 caggttcacc ccagataatc gggccagtct ggtaatccac aaccttaccg ttacggtcaa
      781 catacttacc gttggattcc atattgccct gctggaagta cgccgcgaaa cggtgcatat
      841 actggtcata cggcagaatc gcttcagttt ccgcgccaaa gaaattgttg taccagatgc
      901 caatcagcgc cagcaagaca ggcaggtttt tctcggcagg cgtggtggag aaatgcttgt
      961 ccatcgcgtg tgcgccagaa agcagttcaa caaaattatc aaagccgatg gagagaacaa
     1021 tcgacaggcc aattgctgac cacaaagagt aacggccgcc aacccagtcc cagaactcga
     1081 acatgttggc agtatcaata ccaaactcgc caacggcttt ggcattggtg gaaagcgccg
     1141 caaagtgttt cgcaacgtgt ttttcatcac cagccgcttt caggaaccag tcacgcgcgc
     1201 tgtgggcgtt ggtcatggtt tcctgagtgg tgaaggtttt agatgctacc aggaacagcg
     1261 tggtttccgg gtttactttt ttcagcactt ccgcgatgtg agtcccatcg acgttagaaa
     1321 caaagtgcat gttcaggtgg tttttgtatg gacgcagtgc ttcagtcacc atgtacgggc
     1381 cgaggtcaga accgccgatc ccgatgttca ctacgtcagt gattgctttg ccggtatagc
     1441 ctttccactc accggaaata atcgcttccg agaaggtttt catcttctcc agcaccgcgt
     1501 tgacttctgg cattacgtct ttgccatcaa ccagaatcgg ggtattgcta cggttacgca
     1561 gcgctacgtg cagcacggcg cggttttcag tgcggttgat cttctcgcca gagaacatcg
     1621 acttaatcgc gcccgccaga tcgcactctt tcgccagatc ttgtaatttc gccagcgtct
     1681 cttcagtgat gcggtttttg gagtaatcca ccagcatctg atcgtcgaat gttgcggaga
     1741 acttagaaaa acgatcgccg tctttagcaa aaagatcggc gatcgtaacg tctttcattt
     1801 catcgaagtg tttctgtagt gcctgccagg cagcggtctg cgttggattg atgtttttca
     1861 ttagcaatac tcttctgatt ttgagaattg tgactttgga agattgtagc gccagtcaca
     1921 gaaaaatgtg atggttttag tgccgttagc gtaatgttga gtgtaaaccc ttagcgcagc
     1981 gaagcattta ttagctgaac tactgaccgc caggagtgga tgaaaaatcc gcatgacccc
     2041 atcgttgaca acggccccgc tcacccttta tttataaatg tactacctgc gctagcgcag
     2101 gccagaagag gcgcgttgcc caagtaacgg tgttggagga gccagtcctg tgataacacc
     2161 tgagggggtg catcgccgag gtgattgaac ggctggccac gttcatcatc ggctacaggg
     2221 gctgaatccc ctgggttgtc accagaagcg ttcgcagtcg ggcgtttcgc aagtggtgga
     2281 gcacttctgg gtgaaaatag tagcgaagta tcgctctgcg cccacccgtc ttccgctctt
     2341 cccttgtgcc aaggctgaaa atggatcccc tgacacgagg tagttatgtc tgaaattgtt
     2401 gtctccaaat ttggcggtac cagcgtagct gattttgacg ccatgaaccg cagcgctgat
     2461 attgtgcttt ctgatgccaa cgtgcgttta gttgtcctct cggcttctgc tggtatcact
     2521 aatctgctgg tcgctttagc tgaaggactg gaacctggcg agcgattcga aaaactcgac
     2581 gctattcgca acatccagtt tgccattctg gaacgtctgc gttacccgaa cgttatccgt
     2641 gaagagattg aacgtctgct ggagaacatt actgttctgg cagaagcggc ggcgctggca
     2701 acgtctccgg cgctgacaga tgaactggtc agccacggcg agctgatgtc gaccctgctg
     2761 tttgtcgaga tcctgcgcga acgcgatgtt caggcacagt ggtttgatgt acgtaaagtg
     2821 atgcgtacca acgaccgatt tggtcgtgca gagccagatg tagccgcgct ggcggaactg
     2881 gccgcgctgc agctgctccc acgcctcaat gaaggcttag tgatcaccca gggatttatc
     2941 ggtagcgaaa ataaaggtcg tacaacgacg cttggccgtg gaggcagcga ttatacggca
     3001 gccttgctgg cggaggcttt acacgcatct cgtgttgata tctggaccga cgtcccgggc
     3061 atctacacca ccgatccacg cgtggtttcc gcagcaaaac gcattgatga aatcgcgttt
     3121 gccgaagcgg cagagatggc aacttttggt gcaaaagtac tgcatccggc aacgttgcta
     3181 cccgcagtac gcagcgatat cccagtcttt gtcggctcca gcaaagaccc acgcgcaggt
     3241 ggtacgctgg tgtgcaataa aactgaaaat ccgccgctgt tccgcgcgct ggcgcttcgt
     3301 cgcaatcaga ctctgctcac tttgcacagc ctgaatatgc tgcattctcg cggtttcctc
     3361 gcggaagttt tcggcatcct cgcgcggcat aatatttcgg tagacttaat caccacgtca
     3421 gaagtgagtg tggcattaac ccttgatacc accggttcaa cctccactgg cgatacgttg
     3481 ctgacgcaat ccctgctgat ggagctttcc gcactgtgcc gggtggaagt ggaagagggt
     3541 ctggcactgg tcgcgttgat tggcaatgac ctgtcaaaag cctgcggcgt tggcaaagag
     3601 gtattcggcg tactggaacc gttcaacatt cgcatgattt gttacggcgc atccagccat
     3661 aacctgtgct tcctggtgcc cggcgaagat gccgagcagg tagtgcagaa gctgcatttt
     3721 aatttgtttg agtaaacaca gtatggcccg gaagacatta tttcgggccg tattgttttt
     3781 cttgtcatta tgcccataaa taaacgagcc tgtactctgt taaccagcgt ctttatcgga
     3841 gaataattgc ctttaatttt ttatctcttt ctctaactaa ttagagaaag agataaatag
     3901 ttaagagaag gcaaaatgaa tattatcagt tctgctcgca aacgaattcc cgtacactga
     3961 actctctggc tcactgaggg caccatcatg gcactccccc gtattaccca aaaagagatg
     4021 actgaacgcg agcagcgtga acttaaaaca ctgctggatc gggcgcggat tgcgcatggt
     4081 cgcgtactga ccaactccga aaccaacagc attaagaaag agtacatcga taaattaatg
     4141 gtcgagcgtg aggctgaagc gaaaaaagcc cgccaattga agaaaaagca ggcttataaa
     4201 ccggatcctg aggcgtcatt tagctggtca gcaaatacgt cgacgcgcgg caggcgctga
     4261 ttaacgcccc ttctttttgc gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg
     4321 accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc ctaccgtttt
     4381 cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga gcttaaagag
     4441 gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc cgcttaaact
     4501 gacgttcata atgcgcgtac gttccagctt tttcacttcg taaccaaagt gctcgcacat
     4561 gcgacggatc tgacggttca gcccctgcac cagggtaatg cggaagacaa atggcgcttc
     4621 ttttttgact ttgcactttt tagtcactgt cccgaggatt ggcacccccg cccccatacc
     4681 acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt tctcatgatc
     4741 gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga aaatcagccc
     4801 ctgggagtct ttatccaggc ggccaatcgg gaatacgcgt ttgctgtggt taacgaaatc
     4861 gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg gcttgttcag
     4921 ggcgataagt accaaatctt cggcttcccg aggttcaatc aactgaccat ttactttcac
     4981 aacgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa ggaacacatt
     5041 gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta
     5101 tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac gcggaatata
     5161 ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta aatgtgatct
     5221 gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc ctggcaaaat
     5281 aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac tacgcagaga
     5341 aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa atcgctttga
     5401 aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt tgcacaaatg
     5461 tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt gtgaagattg
     5521 cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt
     5581 accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg
     5641 ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag caaaagtttg
     5701 gcctgaaaga cgttgtggtg gtgtcaggaa atgatgagga tgaagagact caactggcga
     5761 tgatggggtt acacggcgcg caactgctgg atcgcttgct ggaacctggc gatattgtcg
     5821 gtttttcctg gggccgcgcg gtgaacgcac tggttgaaaa cttgccgcag gcggggcaat
     5881 cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc gaaagccgct
     5941 atcatgtaaa cacattaacc tacagcgcgg cagcgaagtt gaaaggggaa tcgcatctcg
     6001 cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg cagtctcagc
     6061 actttaaaac catctctgcc tactgggata atctggatgt cgccctggtg ggaattggct
     6121 caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa gagagtgacg
     6181 acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat attcacggcg
     6241 caatggttga aacaaatatg agcgaaaaaa cactctctat cgaaatgaat aaattaaagc
     6301 aggcgcggta ttccattggc attgccatga gtgaagaaaa atacagcgga attattggtg
     6361 cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct gaactattac
     6421 tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc tctattatct
     6481 aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc attattgtca
     6541 ccggtggcgc atccggcatt ggtctggcga ttgtggatga attattagca caaggcgcga
     6601 atgtacagat ggtcgatatt cacggtggcg atggacaata tgaaggtcat aaaggctatc
     6661 agttttggcc gaccgatatt tccagcgcca aagaggtaaa tcatacggta gcggaaatta
     6721 tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc
     6781 tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct gcattcgaaa
     6841 aaatggtcaa tatcaaccag aaaggcgttt ttctgatgtc gcaggcggtg gcgcgacaga
     6901 tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg ctggaaggct
     6961 cagaaggcca aagctgttac gccgctacca aagccgcgct taatagcttc acgcgatcct
     7021 ggtcgaaaga attgggtaaa cacggtatcc gtgtagtcgg tatcgcgccg gggattctgg
     7081 aaaaaaccag gctacgcacg ccggaatatg aagaggcgct ggcgtggacg cgcaatatca
     7141 ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg cgcgccggaa
     7201 gattagcaga agtcactgat tttgtttgtt atctgctgtc tcaacgcgcc agctatatca
     7261 ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc tttatggtca
     7321 acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg gttcagatgg
     7381 tttacggcaa cgccaatgtt gaagccgttg aatttgtgcc cggtgaaaac gccggagaca
     7441 tcgtagcaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg ctgattgccg
     7501 tggatttaca gtgcggcagc ccgtggaatg cggcagcgat gcttgcgatg cgcaatcctc
     7561 gtctgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt gataaccagc
     7621 acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg
     7681 ttgtctggaa acaactggca acggcagagg aggatttctg atgaacatta cgctcgcccg
     7741 cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag tggctaatgc
     7801 ccagcacatt attatctgca atgacgaagt ttataacgat gaagtgcgcc ggacattatt
     7861 gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc
     7921 ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca ctcgcccaca
     7981 ggatgcctta gcgatggttc gccagggggt aaaaatagac accctgaata ttggcggcat
     8041 ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg atgatgatat
     8101 taacgcattt catgagttaa ataatcttgg cgtcatactg gatttacgcg tcgtagcgtc
     8161 agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa attaaataat
     8221 agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag gtgacaaatc
     8281 atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat tgccggaatg
     8341 ggcagcgtgc tggatgaatt tcagactcat cgtccgttaa ttgcctgtac ggtgattggt
     8401 ttaattctcg gtgatttaaa aaccggaatt atgctcggtg gtacgctgga attgatagct
     8461 ctcggctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc cagcataatc
     8521 tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg
     8581 cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac cgtagtgttc
     8641 cagcacgcgg cggataaagc agcggaagaa gcacggtttc gcactctcga tattctgcat
     8701 gtctccgcgc ttggcgtgca ggcactgcgc gttgctattc cggcactgat tgtctcgctg
     8761 ttcgtcagcg ctgatatggt gagcaatatg ctgagcgcca ttcccgaatt tgtgacccgt
     8821 ggactgcaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt gcttcgcatg
     8881 atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg tggctacctc
     8941 gatctcagtc tgctggcgtt cggtggcgtc ggcgtgatca tggccctgct ctacatccag
     9001 ctaaatccac agtggcgtaa agctgaacct caactccagg ccaccgcctc caccgccctt
     9061 gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac gcagcgatct
     9121 ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg aacgtattca
     9181 cgggctgggt ttttgctacg acatgatccc cgccatcaag cgactttacc cattaaaaga
     9241 ggatcaggtt gcggcgctca ggcgacacct ggtgttcttc aataccacgc cagccgtatg
     9301 tggcccggtc atcggcgtca ctgccgccat ggaagaggcg cgggccaacg gcgcggaaat
     9361 tgatgacggt accattaacg gcatcaaagt cggtctgatg ggaccattgg caggagttgg
     9421 cgatccactg gtctggggaa cgctgcgccc gattaccgcc gcgctcggcg catctctggc
     9481 actttcgggc aacattctcg gcccgctgct gtttttcttt attttcaatg cggtgcgtct
     9541 ggcgatgaag tggtatggcc tacagctcgg ctttcgcaaa ggggtgaata tcgtcagcga
     9601 tatgggcggg aatgtgctgc aaaaactcac cgaaggcgcg tcgattctcg gactgtttgt
     9661 gatgggcgtg ctggtgacca aatggacgtc aatcaacgta ccgttgctgg tttcacaaac
     9721 gcatgccgcc gatggctcca ccgtcaccat gaccgtgcag aacattctcg accaactttg
     9781 ccctggtttg ctggcgctcg gtctgacgct gctgatggtt cgtctgctca acaaaaaaat
     9841 taacccggta tggctgattt tcgccctgtt tggtttaggg attatcggta atgcgctggg
     9901 cttcctgtcc tgattcttcg ccccggcacg actgccgggg tcatcgctca acatgaggtg
     9961 gtttatgaaa acaacagctc tgcgtcttta tggcaaacgt gacctgcgtc tggaaacctt
    10021 tgaccttcct gaaatgcagg aggatgaaat cctcgcaacg gtggtcactg acagcctgtg
    10081 cctctcttcc tggaaagagg ccaatctggg tgaaaaccat aaaaaagtac ccgacgatgt
    10141 ggcgaccaac cccatcatca tcggccacga gttttgcggc gatattctgg ccgtgggtaa
    10201 aaagtggcag cacaaattcc agccgggtca gcgttatgtg attcaggcca acctgcaact
    10261 ccccgaccgc ccggattgcc ccggctactc cttcccgtgg gtaggcggcg aagcgacgca
    10321 tgtggttatt cccaacgagg tcatggaaca agattgcctg ctggcatacg acggcgaaac
    10381 ctattttgaa ggctcgctgg ttgaaccgct ttcctgcgtg attggcgcgt tcaacgccaa
    10441 ctatcatctt caggaaggta gttataacca cacgatgggg attcgcccgc aagggcgcac
    10501 gctgatcctc ggcggcaccg gcccgatggg gctgctggcg attgattatg cgttgcatgg
    10561 accggttaac ccgtcgctgc tcgtcattac cgataccgac aacgacaaat tgagttatgc
    10621 acgcaagcac tatccatcag aaccgcaaac actgattcat tatctcaatg ccgccgatgc
    10681 agcatttgat acgttaatgg cactgagtgg cggtcacggc ttcgatgata ttttcgtctt
    10741 tgtgcctaat gaaggactgg tgactctcgc ctcttccttg ctggcgacag atggttgtct
    10801 gaatttcttc gccggaccgc aggataagca cttcagcgcg ccaattaatt tctacgatgt
    10861 gcattatgca tttacccact acgtgggcac gtcaggcggc aataccgacg acatgcgcgc
    10921 agcggtcaaa ttgattgaag agaaaaaagt gcaggccgca aaagtggtaa cacatattct
    10981 tgggctgaat gccgcgggcg aaaccacgct tgaattgcct gccatcggcg gcggcaaaaa
    11041 gctggtgtat accgggaaat acctgccgct tatgtcactc acgcagattc aggatcaagc
    11101 actggcggca attctggcgc gtcatcaggg gatctggtcc ggtgaggcgg agcaatacct
    11161 gctcgcccat gcagaggcaa tttcccatga ttaatcgcgc tacttaactc tgcatgtcgc
    11221 tggctggtcg ccgcggcaac ttcgcgacct gatgtgcggc tgattgctga agcggcacaa
    11281 tgtgcacgag aaggataaaa aaacatcagc gccttaaggg gcgctcaaat tggtggcaaa
    11341 gagcacattg tttatgccgg atgcggaatg aagccttatt cggcctacat aatcttgaaa
    11401 attcaatata ttgcagttat ctggtaggcc aggtaagcgt agcgcatcag gcaatcctgt
    11461 gtttgtcatc agtctcagcg cctgtgcaat atgtgacatc gtgagccatg tcagtaagtg
    11521 gttaaattcg cagtggtagc attccttccg tgttttctta ctaaccggag aagtaatgga
    11581 actgctttta ttgagtaact cgacgctgcc gggtaaagcc tggctggaac acgcgctgcc
    11641 gctgattgct gaacagttgc agggtcgccg ttctgcggta tttattcctt tcgcgggcgt
    11701 aacgcagacc tgggatgatt acacagcgaa aacggctgcg gttctcgctc cgctgggtgt
    11761 ttctgtcacc ggtattcata gcgttgtcga tcccgttgcc gcgattgaaa atgcggaaat
    11821 tgtgattgtc ggcggcggta atactttcca gttgctgaaa cagtgccgcg agcgcggact
    11881 gctggcacca attactgacg tggttaaacg tggcgcgctg tatattggct ggagcgctgg
    11941 cgctaacctt gcttgcccaa ccattcgtac caccaacgat atgccgattg tcgatccaca
    12001 aggtttcgat gcgctgaatc tgttcccgtt gcaaatcaac ccgcacttca ccaacgcgct
    12061 gccggaaggc cataaaggtg aaacccgtga gcagcgtatt cgcgaactgc tggtcgtcgc
    12121 gccagaactc acaattattg gtctaccgga aggtaactgg atcacggtga gtaaaggtca
    12181 cgctacgctg ggtggcccga acaccactta tgtgtttaag gctggtgaag aagcggttcc
    12241 gctggaagcg ggtcaccgtt tttaagtgaa ttgccggatg catgatccgg catcttgtac
    12301 atagtgcctg atgcgacgct ttacacgtct tatcaggcct tcagggtcac ggtattaata
    12361 ctcgtcccgt ccttcgtctt catccggctg ttccagcaca ctgtaagcca ccgaacaaaa
    12421 cagcgagttc agacgctgca tatctcccag taagcctaaa tgtagcgaac tggtttcaat
    12481 actctgcacg ttttgctgat gcaggcgatc gacgtgagca tgggaataac ggcgattaag
    12541 aatgcgaaag cgatgcttgc tgcggcgcaa acgacgggcg ctggtaacat cgccagagaa
    12601 gaaaaccgac attgccagtt ttaaattact gagcaattgc tcatagagcg catccagttc
    12661 cttcaagcca tcaagcgaaa atgcccgccg tgctgccagc gatttatcgg caatttcact
    12721 ccccatgcgc tcgacgatat cggaggcctg ttcaaggttg agcgacattt cgatgatctc
    12781 cgcccagcgg cgcgactctt cttctgccag ttcctctttt ggcatccgcg ccagatacag
    12841 cttaatggcg gtatagagaa cgttgatgtc atcagccagc ttacgcagct ctttctcctg
    12901 ccgtggctcg ccgtgcatca ctttattcaa cccttccatc atctgttcca tggcgtcgcc
    12961 aatgcgcagg gtttcgcggg cagcgttcgc cagagcaagc gtgggcgtat ccagcgcgct
    13021 gacatccaga tgtttaggcc gtagctgggt atccagttcc ggttcatcgc gaataatcgt
    13081 tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg accaggcagc gtacaaggtt
    13141 gtagaagacg tggaaataga tcaccaattc cgcttttggc aatggcaact tccccattgt
    13201 ctctgccagc aaatgaacaa acggcaggat aatcaggcta cccaccagct taaacagcag
    13261 gctacccagc gcgacacggc gggctgcggc attggcggca ctgttgttga gcatcgccag
    13321 caggccggaa ccgaggtttg caccaatcac cagacaaagc gccactggga aagagataat
    13381 gcctgcggcg gtcagagtcg cggtcagcag taccgcggca aggctggagt agctgataat
    13441 ggcgaacatc gcaccaatca gcgcatccag cagaatatcg ccggtcagcg aggcaaagat
    13501 cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc tgcacaatca actccagcgc
    13561 cagcaaaatc aacccaagac caataccgac gcggcccagt tgcccggcgc gtgactgttt
    13621 gcgtccgagg aaaaaaatca cgccgataaa aataagtaat ggtgacagcc aggataagtc
    13681 gaaggtgaga atacgcgcca ttagcgccgt cccgacatct gcacccagca caatcaccag
    13741 agccggtgcg agggctacca gatcctgagc gacgaacgag gtcaccagca tagtggtggc
    13801 attactgctc tgcaccagtg cggtaacgcc tatccccgcg caaaaggcga gcggcttttt
    13861 ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg aagacgcgca ttacgccggt
    13921 tcgaacaata tgagtccccc agaccagcag ggcgacggca gaaagcaggt gaagcagcgt
    13981 taacacgagt tcaggttctc cttatcgtta tatgttcctg aagcaattga atgaccaaag
    14041 gaccggaaac acgctgactg gcatctttac tgtgcctttt gaccttcgtt tttccggcta
    14101 agattattcc agcctgtttc cagtataagt gtttcatatt aataaagaga cagggcactc
    14161 attgagcgcc ctgtttgttg taaggaaaag tgacagattt gtgaatcagt ccgcgtcata
    14221 ccccagattc ggtgccagcc agcgctcaac atctgagacg ctcatacctt tacggcgggc
    14281 atagtcttca acctgatcgc gctggatttg cgccaccgcg taatacttgc tgtccgggtg
    14341 gctgaagtac caacccgaaa ccgatgcacc aggccacatg gcgaaagatt ctgtgagttt
    14401 catgccagtg tgtttttcca cttccagcag ctcccagatg gtggcttttt ccgtatgttc
    14461 cgggcaggcc ggataacccg gtgccggacg gatgccctgg tagttttcgc ggatcagctc
    14521 ttcgttgctg aggttctcgt tcggcgcata gccccagtag actttacgca cacgctcatg
    14581 gagatactcc gcaaaggctt cggctaaacg gtcggcaagc gctttcacca tgattttgtt
    14641 gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg tcctcttcca gcccgccagt
    14701 cacggcaaat gcgccgatgt aatctgcttt accagaaagc ttcggcgcaa cgaagtcagc
    14761 gagacagtag ttagcgaagc cggttttttc ggtctgttga cgcagatggt ggctgacgtt
    14821 gatcacatgg gtacgcgttt cgtcacggta gatttcaatg tcatcgccca cacggtttgc
    14881 cgggaacagg cccaccacgc cacgcggatt cagcgttttt tcggcgctta atttatccag
    14941 catgtcgttg gcgtctttaa acagccgctg cgcctcaacg cccaccactt catcttccag
    15001 aatgcgcgga tacttcccgg acagcgacca ggtcataaag aacggtgtcc agtcgatgta
    15061 attacgcagc gtttcgatgc tggcttcgac ttcctgcaca ccgagacggt gcgccaccgg
    15121 cggcgtgtaa gcctgccagt caaaagcgaa gtcgttatca cgcgccgctt ccagcgtgac
    15181 cggtggtgtg cgtggtttct ttcgtccgtg ctgaatacgc acggtttcgt actccttgcg
    15241 ggtacgagca acgaaatcat cgcgctgagt gtcggaaagc aacgccgcca ccacaccaac
    15301 ggtgcgtgaa gcgttctgca catacaccgt cgggccgctg tagttctgct caattttcac
    15361 cgccgtgtgc gcttttgagg tggtcgcgcc gccaatcaat agcggaatgg tgaagccctg
    15421 acgctccatc tctttcgcca cgttaaccat ctcgtccagc gacggcgtga tcagccccga
    15481 aaggccaatc aaatcagcat tcacttcttt agcggtacgg agaatttttt ccgcaggcac
    15541 catcacgccg agatcgacaa tttcgtagtt gttacattgc agcaccacgc caacgatatt
    15601 tttaccgatg tcgtggacat cgcccttcac tgtggcgatc accatcttgc cgttggtttt
    15661 accctgctct ttgctggctt caatgaacgg ctcgagatac gccaccgcct gtttcatgac
    15721 gcgcgccgat ttcaccacct gcggcaggaa cattttgccc tcgccaaaca ggtcgccgac
    15781 cacgttcatg ccgtccatca acgggccttc aatcacttca atcggacgcg tagcctgctg
    15841 gcgggcttct tcggtatcct gctcgataaa ctcggtaatg cctttgacca gcgagtattc
    15901 cagacgttta ttcacttccc acgagcgcca ctctgcctgc tgggtgttag cggtgtcgtc
    15961 ggttttgctg ccgcgatatt tctcggcaag ctcgagtaaa cgctcggtgc catcgtcgcg
    16021 acggttgagg atcacatcct ccaccgcgtc gcgtagctcg gtaggcaggt cgtcgtaaat
    16081 cgccagttgc ccggcgttga cgatccccat atccatgcca ttgcgaatag cgtagtagag
    16141 gaacaccgcg tgaatggctt cgcgcaccgg atcgttgcca cggaatgaga aagaaacgtt
    16201 agaaacgccg ccggaaatca gtgcgtgcgg cagttcacgt ttgatgtctt cacacgcgcc
    16261 gataaagtcc tgcgcgtaat tgttgtgctc ttcaatgcca gttgcgaccg cgaagatgtt
    16321 cgggtcgaag atgatatctt cagccgggaa gccaacctct tcggtgagga ttttgtacgc
    16381 ccgacggcaa atctcgattt tccgtgcgcg ggtatcggcc tggccctgtt cgtcaaaagc
    16441 cattaccacg actgccgcac cgtagcgacg caacagtttc gcgtgatgga taaaggcgtc
    16501 gacgccctct ttcatcgaga tagagttaac aatgcctttg ccctggatac acttcagacc
    16561 tttttcaata acgtcccatt ttgaggagtc gatcattatc ggcacgcgag cgatatccgg
    16621 ttcaccggca atcagattga gaaaacgcac catcgccgct tcggcgtcga gcatcccttc
    16681 atccatgttg atatcgataa tctgcgcacc gttttccacc tgctgacgcg ccacatccag
    16741 cgcctcgctg tatttctctt ctttgatcag gcgcttgaac ttagcggaac cggtgacgtt
    16801 ggtgcgttca cccacgttca caaacaggct atcttcgcca atgttcagcg gctccaggcc
    16861 ggacaaacgg caggctacgg ggatttccgg cagtttgcgc ggcgctaatc cttctactgc
    16921 acgactcatc gcggcaatat gttgtggcgt ggtgccacag cagccgccga cgatattgag
    16981 aaagcccgct tgcgcccatt cacgtatctg ttttgccatc gtgtcggcgt cgagatcgta
    17041 ctcaccaaag gcgttgggta gcccggcgtt cgggtgcgct gtgacgtagc attccgcaat
    17101 ccgtgataat tcctgcacgt actggcgcag ttcgtctggc cccagcgcac agttaaggcc
    17161 gaaagtcagc gcttcggcgt ggcgcagaga gttataaaac gcttcggtgg tctgcccgga
    17221 gagcgtgcgc ccggaggcgt cggtgatggt gccggagatc ataatcggca gctcaacgcc
    17281 cagcgcttca aactccgttt tcaccgcaaa gaccgccgct ttggcgttaa gggtgtcgaa
    17341 aacggtttca atcagaatca ggtcggcacc accttccacc agcgctttgg tggactctcg
    17401 ataagccgcc accagcccgt caaaagtgat attacgaaat gccggatcgt tgacatccgg
    17461 agaaatagac gccgtgcggt tggtcgggcc gagaacaccg gcaacgtagc gcggtttctc
    17521 cggcgtgcgc gcggtccact catcagcaca ggcgcgcgcc agtttcgccg ccgcaaagtt
    17581 gatttccgcc gacaggaatt ccatctggta atccgccatc gcaatggttg tggagttgaa
    17641 ggtgttggtt tcgatgatat ccgcgcccgc ttcaaagtag gcgttgtgga tagcggcgat
    17701 cacttccggt ttgctgagca ccagcaggtc gttgttgcct ttgagatcgc atggccagtc
    17761 ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga tagctctgga tcatggtgcc
    17821 cataccgccg tccagcacca gaatacgttc atttaactgc gcacgcagtt gttccacttt
    17881 gctgctcaca cttgctcccg acacgctcaa cccagattta ataaaaattc aacaaaccat
    17941 actggcataa acgcatctgt ggtaaaagcg accaccacgc aacatgagat ttgttcaaca
    18001 ttaactcatc ggatcagttc agcaactatt gcattagcta acaataaaaa tgaaaatgat
    18061 ttccacgata cagaaaaagg agactgtcat ggtcgcaccc attcccgcaa aacgcggcag
    18121 aaaacccgcc gttgccaccg caccagcgac tggacaggtt cagtctttaa cgcgtggcct
    18181 gaaattactg gagtggattg ccgaatccaa tggcagtgtg gcactcacgg aactggcaca
    18241 gcaagccggg ttacccaatt ccacgaccca ccgcctgcta accacgatgc agcaacaggg
    18301 ctttgtccgt caggtcggcg aactgggtca ctgggcaatc ggcgcacacg cctttatggt
    18361 cggtagcagc tttctccaga gccgtaattt gttagcgatt gttcacccta tcctgcgcaa
    18421 tctaatggaa gagtctggcg aaacggtcaa tatggcggtg cttgatcaaa gcaatcacga
    18481 agcgattatt atcgaccagg tacagtgtac tcacctgatg cgaatgtccg cgcctatcgg
    18541 cggtaaattg ccgatgcacg cttccggtgc gggtaaagcc tttctggccc aactgagcga
    18601 agaacaggtg acgaagctgc tgcaccgcaa agggttacat gcctataccc acgcaacgct
    18661 ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg cgcaaacggg gttattcatt
    18721 tgacgatgag gaacatgcgc tggggctgcg ttgccttgca gcgtgtattt tcgatgagca
    18781 ccgcgaaccg tttgccgcaa tttcaatttc cggaccgatt tcacgtatta ccgatgaccg
    18841 cgtgaccgag tttggcgcga tggtgattaa agcggcgaag gaagtgacgc tggcgtacgg
    18901 tggaatacgc tgactttttc gcctctgatt tatacgaatc gaacgctaaa ccgctgcttg
    18961 cgccgatacg cataaacatc ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc
    19021 cagtaatcag cgcggaacaa gtcggcgtgc atctcttcaa atagcggacc aatacgcggg
    19081 tcggcacata accagtgacg gaactcttcc gggaaaacat cgcccggcga gacgctgtac
    19141 cacggttcgc tggcaagttc gtcctccggg tagcgcggcg gcgggatgtc gcggaagttc
    19201 acttccgtca tgtagcaaat ttcgtcgtaa tcataaaaca ccacgcgccc gtgacgggtg
    19261 acaccgaagt ttttaaacag catgtcgcca gggaaaatat tagcggcggc aagctggcga
    19321 atagcgttac cgtattcttc aatggcatcg cgcaactgct gaccttccac ttgttccagc
    19381 cagatattga gcggcaccat ccgccgctca atataaagat ggcgaatcac aatttgttcg
    19441 ccgagatcgg tgattttttc cgctgcttcc tggagcagta atgccattaa tgccggggaa
    19501 atatgccgct tctccagcac aaagttttca aactcctggg tgtccgccat tcggcccacg
    19561 cgatcgtgct ctttcaccag ttgatagcag gcacgaacgt gagcggcaga catctctttc
    19621 tgcggcgcga acttgtcttt gatgactttg aatacccgat caaagcccgg cagcgtaaac
    19681 accagcatca ccatgccacg aatacccggc gcttcaatga actgctcatt acagccctgt
    19741 agataaacga gatattcgcg gtagctttcg gttttggcgt gcttctggca gccgatagcc
    19801 atatacaatt cagcggtggt tttacctggc agaatttccc gtagccactc gaccagcgct
    19861 gcgggcagcg gtgcataaac cataaaataa gaacgcgcaa agccaaaaac aatgctcgct
    19921 tcggcggtgg tcgtcaggca ggtatcaata aataactcgc cgtcgtccgt ctggtggatc
    19981 ggcagcaaaa atggcaatgt gccggaaggc gtgatcagat tgcctaccag ccaggcggct
    20041 ttattgcggt aaaacagttc gttcgccacc tgtaaatgac tttccgcgag gttgtctgtc
    20101 cccagcgttt ccgtcagatg gcgaacgatg taatgaatgt cacggctttt attctgccaa
    20161 cgcagacgca gcggcagatc gctgataacg cgcatcagca gagattccca gccgtgatcg
    20221 gggtgaaagt ctttcgccag cgggcgggga atggtacgaa aacggcgctc tggctgggag
    20281 ctaaagataa aaagccgctc gggagtaagc gagcggtggt caaataacct acagtacacg
    20341 gagttgaaaa agctctccgc aatctcgaag cgcgggtaat ccggcaacag ccgcgtgtaa
    20401 tgctctttga cgcgcagtaa aaatgccgcg tccgtgcttt ggccgttggt aatgcagcgc
    20461 agttgctcca cgaccagacc cacgtgatga tcgtaaagat gaatacggtt tttcatcgcc
    20521 tgctggacag catgccagtc ggcttgctcg aaacgctgct gcgccccgga agtcacttcg
    20581 aggaatcgac catactgagc gtcgaaacct tgcaaaatgg tttgtgcaat cagtaattcc
    20641 aggccacgcg gcatttgttg ccctcgtcgg gagcagagaa ttgccggata agacgcgtaa
    20701 gcgtcacatc aggcgctgat tgccggatgc ggcgtagacg ccttatccgg cctacgttcg
    20761 gcaacggctg taggcatgat aagacgcgca agcgtcgcat caggcgctga ttgccggatg
    20821 cggcgtgaac gccttatccg gcctacagtc agcaacgttt atcggttgct tagaactgcg
    20881 attcttcggt ggagccagtc agtgcggtaa ccgacgacgt gccgccctga ataatggtcg
    20941 tcactttatc gaagtaacct gtacccactt cctgttggtg agatacgaag gtatagccat
    21001 ctttcgcggc ggcaaattcc ggctgctgca ctttctcaac atagtgcttc ataccctcgc
    21061 cctgggcata ggcgtttgcc aggtcaaaca tgttgaacca catgctgtgg atacctgcca
    21121 gggtgatgaa ctggaacttg tagcccatat ccgacagctg ctgctggaag ctggcaatag
    21181 ttttgtcgtc gaggtttttc tgccagttga acgacggcga gcagttataa gccagcagtt
    21241 tgcccggata tttcgcgtgg atggcttgtg caaagcgacg cgccagttcc aggtccggcg
    21301 tggaggtttc acaccagacc aggtcagcat atggcgcata cgccaggcca cggctgatcg
    21361 cttgctcaat acccgcatga gtacggaaga agccttcact ggtacgctcg ccagtaataa
    21421 attcgctgtc atatgggtcg caatcggagg taatcagatc cgccgcatcg gcatcggtac
    21481 gagcaaccag cagggttgga acgcccgtca catcagctgc cagacgcgcc gcgaccagtt
    21541 tctgaatagc ttcctgagtt ggcaccaaaa ctttgccgcc catgtgacca catttcttca
    21601 ctgatgccag ctgatcttcg aagtgaactg ccgctgcacc ggcttcaatc atcgctttca
    21661 tcagttcaaa ggcattcagt acaccgccaa aaccggcctc cgcatcggcg acgatcggca
    21721 ggaagtaatc gacatagcgc ggatcgcccg gctcaatgcc cgcggaccat tggatctgat
    21781 ccgcacggcg gaaagtattg ttgatccgct ccaccaccgc tggcaccgag tttgccggat
    21841 agagcgactg atccggatac atgctggccg ccaggttagc gtccgccgct acctgccatc
    21901 ccgacagata gactgcttca atacccgctt tcgcctgttg cagcgcctga ccgccagtca
    21961 gtgcaccgag gctgttgatg tagccttttt tcgactcgcc gtgcagcaga cgccacattt
    22021 tcgccgcgcc cagttgcgcc agggtgcatt ccggattgac tgaaccgcgt aatttcacca
    22081 catcttccgc gctgtacggg cgagtaatgc cttcccaacg cggttgagtc cactctttct
    22141 gtaattcttc aatttgttgt gtacgggttt tcatgtgcag atgctccata gttatgtggt
    22201 ggtttacgct aacaggcggt agcctggcag ggtcaggaaa tcaattaact catcggaagt
    22261 ggtgatctgt tccatcaagc gtgcggcatc gtcaaaacgc ccctgggaga aacgttcttc
    22321 gcccagttcg ctggcaatga ctttcatctc ttcgcccagc atctggcgga acaaggcttt
    22381 agtcaccggt ttgccattgc tcaacgtttt ttgatgatgg atccactgcc agattgaggt
    22441 acgggaaatt tcagccgtcg ccgcatcttc catcaggcca taaatcggca cacagccatt
    22501 gccagagatc cacgcttcga tgtactgcac agccacgcga atgttggcgc gcataccttc
    22561 ttcggtgcgt tcaccatcac aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg
    22621 ttcgcgcatc acttcaagct gatttttacg ggagccgaga atgtcgttga ataccgccat
    22681 tgccgtatcc gcaaggcctg ggtgggcaat ccatgtgcca tcgtgaccgt tattagcttc
    22741 cagcgattta tccgctttta ctttgttgag cacctggtta ttgcgctctt catctttgct
    22801 cggaataaat gccgccatac cgcccatcgc aaaagcaccg cgtttatggc aggttttaat
    22861 caacaggcgt gagtaagcat tcaggaaggg tttatccatc gtcactgcct gtctatctgg
    22921 caggacgcga tcgggatagt ttttcaatgt tttgatataa ctgaagatgt aatcccaacg
    22981 accgcagttc agaccaacaa tatggtcacg cagcgcgtga aggatttcat ccatctggaa
    23041 cacggcgggc agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa
    23101 gcgatcttct gcatagctga agacttcgct ccaccaggcc gcttcctgcc aggactgggt
    23161 tttcggcaga tagaaatagg gaccgctgcc ctttgccaac agtgcctgat agttgtggaa
    23221 gaaatagagc gcaaaatcaa acaggctacc ggggattgcc tcaccacgcc aggtgacatg
    23281 tttttccggc aagtgcagac cgcgtacccg acaaatcaaa accgctggat tgggcttgag
    23341 ctgataaatt ttgcctgctt cattggtata gctgatggtg ccgttaaccg catcgcgcag
    23401 gttaatttgc ccatcgatca ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc
    23461 cataaagact ttcacattgg cgttgagcgc gttgatcacc atcttgcgct caaccgggcc
    23521 ggtaatctct acccgacgat cttctaagtc cgcaggaatc ccgcgaattt tccagtcagc
    23581 atcacgaatg gaagctgttt ccgaaataaa atcaggcaac gttccgttat cgatatcctg
    23641 ctgctgctga atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag
    23701 ctcagtcaga aattctaccg cttcggcagt aagaatatgc ttctcctgct cgccatacgg
    23761 ccttgtgaaa gccaattcat cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt
    23821 catggatccg aaaacttccc cactgtgaac gaaggatcgt tgtgcgcttt tcgttcagca
    23881 caactaagac tactcattta aaatgcaaaa tcaaaaacaa tttccatttt aattaaaaat
    23941 acttgataac ttattgataa caatatgaat aaaatttatt cacctactga ggtgcgtttc
    24001 ggaaataaaa aaggcacccg aaggtgcctg aggtaaggtg ctgaatcgct taacgatcga
    24061 ctatcacaga agattaatcc agcgttgggt tcatgtgccg tagatcgtat ggcgtgatct
    24121 ggtagacgta atagttgagc cagttggtaa acagcaaatt accgtgacta cgccagctcg
    24181 ctcgcggtgt attttgcgga tcattatgcg ggaaatagtt atacggtaca tccgggtcta
    24241 gtccggcttc cacatcgcgg aaaaattcct gcgccagcgt ttgcgcatca tattcgggat
    24301 ggcccgtcac aaaggcaatt cgcttatctt tgctggcaaa cagataggca tccccttctt
    24361 ctgtctccgc cagaatttcc agatcggtat aatcacgaat caacgctgcc ggaaagtcag
    24421 catagcgcga atgtggtgcc aggaatgaat catcaaagcc acgcgtcaga agcgcatgag
    24481 gatggagaat atgatgctcg taaacgccag agagtttgtc ggtgcgggtt tgcttaggaa
    24541 tgccgtagag gatattgagc gcggcctgga ccgcccaaca gacaaacagc gtcgaggtga
    24601 cgtgatcttt cgaccactcc agcacctgtt tgatctgcgg ccagtaagca acatcattaa
    24661 actccaccag gcccagcggc gcgccggtaa ctatcagacc atcaaagttt tgttcctgaa
    24721 tatcttcaaa gttacagtag aagttattca gatgctctgc gggcgtgttg cgcgattcac
    24781 gggaatcgat gcgcaacagc tgaatatcga cctgcaaagg tgagtttgaa agcaggcgca
    24841 gaaactgatt ttcagtttcg atcttcttcg gcatcaggtt aaggataagc acctttagcg
    24901 gacgaatttc ctgaccagac gcacgagaag ttgtcatcac aaagacgttt tcttcacgca
    24961 agaaattgac ggcgggtagc tcgtccggca cacgaatcgg cataacctga ttacctcact
    25021 acatacgctt atacgtttag acatccagat agctgaagat aaccagaaaa tttgccaatg
    25081 tcgagcctgc atattgaagg tgagaaagtt tcaaggaaaa ggctacgtta gaatataaga
    25141 atgacgaaaa ggagagaaaa tggttattag tattcgccgc tcacggcatg aggaagggga
    25201 ggagctcgtt gcgatttggt gtcgttctgt cgatgccact cacgattttt tatcagcagc
    25261 gtatcgggcc gagctggagg agctagttcg ttccttcctg ccggaagcgc cgttgtgggt
    25321 cgcggttaat gagcgggatc ggccggttgg gtttatgttg ttaagtgggc agcatatgga
    25381 tgcgctgttt atcgatcctg atgtgcgcgg ctgcggcgtg ggtcggatgc tggtggaaca
    25441 tgcgctctcg atggcaccgg aactgacaac caatgttaat gagcaaaata agcaggctgt
    25501 tgggttctat aagaagatgg gttttaaggt tacgggacgc tctgaggtgg acgatttggg
    25561 gaaaccgtat ccattgctga atctggcata tgtaggggaa taagctgccc cctatcgtgc
    25621 tatcgtgctt gcgtaagctg gagcagacgc gccagcgtcg catccggcat tttttctgct
    25681 ccagcgcaaa aaggccatcc tttcggatgg ccttt
//
LOCUS       DAZUCC010000055        25382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZUCC010000055 DAZUCC010000000
VERSION     DAZUCC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.055"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(204..1016)
                     /locus_tag="ACHY09_001943"
     CDS             complement(204..1016)
                     /locus_tag="ACHY09_001943"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFS3884921.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
     gene            1278..2042
                     /gene="udp"
                     /locus_tag="ACHY09_001944"
     CDS             1278..2042
                     /gene="udp"
                     /locus_tag="ACHY09_001944"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFS3884922.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(2076..3566)
                     /locus_tag="ACHY09_001945"
     CDS             complement(2076..3566)
                     /locus_tag="ACHY09_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFS3884923.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(3579..4085)
                     /locus_tag="ACHY09_001946"
     CDS             complement(3579..4085)
                     /locus_tag="ACHY09_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFS3884924.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(4102..5076)
                     /locus_tag="ACHY09_001947"
     CDS             complement(4102..5076)
                     /locus_tag="ACHY09_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFS3884925.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMAYSKANPGKVRIGN
                     SGTGAIWHLAVAALEDKTGTKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(5101..5730)
                     /locus_tag="ACHY09_001948"
     CDS             complement(5101..5730)
                     /locus_tag="ACHY09_001948"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFS3884926.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(5720..6724)
                     /locus_tag="ACHY09_001949"
     CDS             complement(5720..6724)
                     /locus_tag="ACHY09_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFS3884927.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(6748..7515)
                     /locus_tag="ACHY09_001950"
     CDS             complement(6748..7515)
                     /locus_tag="ACHY09_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS3884928.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            7732..9159
                     /gene="rmuC"
                     /locus_tag="ACHY09_001951"
     CDS             7732..9159
                     /gene="rmuC"
                     /locus_tag="ACHY09_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFS3884929.1"
                     /translation="MDFSIIVYAIIALVSVVIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            9254..10009
                     /gene="ubiE"
                     /locus_tag="ACHY09_001952"
     CDS             9254..10009
                     /gene="ubiE"
                     /locus_tag="ACHY09_001952"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFS3884930.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            10023..10628
                     /gene="ubiJ"
                     /locus_tag="ACHY09_001953"
     CDS             10023..10628
                     /gene="ubiJ"
                     /locus_tag="ACHY09_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFS3884931.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            10625..12265
                     /gene="ubiB"
                     /locus_tag="ACHY09_001954"
     CDS             10625..12265
                     /gene="ubiB"
                     /locus_tag="ACHY09_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFS3884932.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            12344..12613
                     /gene="tatA"
                     /locus_tag="ACHY09_001955"
     CDS             12344..12613
                     /gene="tatA"
                     /locus_tag="ACHY09_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFS3884933.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            12617..13132
                     /gene="tatB"
                     /locus_tag="ACHY09_001956"
     CDS             12617..13132
                     /gene="tatB"
                     /locus_tag="ACHY09_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFS3884934.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            13135..13911
                     /gene="tatC"
                     /locus_tag="ACHY09_001957"
     CDS             13135..13911
                     /gene="tatC"
                     /locus_tag="ACHY09_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFS3884935.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            13953..14735
                     /gene="tatD"
                     /locus_tag="ACHY09_001958"
     CDS             13953..14735
                     /gene="tatD"
                     /locus_tag="ACHY09_001958"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFS3884936.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKVPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(14732..15220)
                     /gene="rfaH"
                     /locus_tag="ACHY09_001959"
     CDS             complement(14732..15220)
                     /gene="rfaH"
                     /locus_tag="ACHY09_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFS3884937.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            15387..16880
                     /gene="ubiD"
                     /locus_tag="ACHY09_001960"
     CDS             15387..16880
                     /gene="ubiD"
                     /locus_tag="ACHY09_001960"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFS3884938.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            16926..17627
                     /gene="fre"
                     /locus_tag="ACHY09_001961"
     CDS             16926..17627
                     /gene="fre"
                     /locus_tag="ACHY09_001961"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFS3884939.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(17819..18982)
                     /gene="fadA"
                     /locus_tag="ACHY09_001962"
     CDS             complement(17819..18982)
                     /gene="fadA"
                     /locus_tag="ACHY09_001962"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFS3884940.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMIGDAQTCLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLTQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISSTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(18992..21181)
                     /gene="fadB"
                     /locus_tag="ACHY09_001963"
     CDS             complement(18992..21181)
                     /gene="fadB"
                     /locus_tag="ACHY09_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFS3884941.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYSGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKMA"
     gene            21371..22702
                     /gene="pepQ"
                     /locus_tag="ACHY09_001964"
     CDS             21371..22702
                     /gene="pepQ"
                     /locus_tag="ACHY09_001964"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFS3884942.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENITRDLKLA"
     gene            22702..23316
                     /locus_tag="ACHY09_001965"
     CDS             22702..23316
                     /locus_tag="ACHY09_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFS3884943.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            23355..24806
                     /gene="trkH"
                     /locus_tag="ACHY09_001966"
     CDS             23355..24806
                     /gene="trkH"
                     /locus_tag="ACHY09_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFS3884944.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            24818..25363
                     /gene="hemG"
                     /locus_tag="ACHY09_001967"
     CDS             24818..25363
                     /gene="hemG"
                     /locus_tag="ACHY09_001967"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFS3884945.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         5986 a         6500 c         6851 g         6045 t
ORIGIN      
        1 tttgagaatg ggagttgatg gtaaatctct aaggtgtctg ccagtgaaga gactcattta
       61 tgactatgac ttgccgactt tcttaaggtc aaatagcgga gtcgtgctct ccaatcaatt
      121 catactgatt attttttggg ggacagagat ggcagcaaaa tgcactcgtt cactcaccat
      181 ctcaaatata gactaagagg aaactaattc cgcttcccac catactgctt aaaccactcc
      241 agcatacgct gccagccatc tttcgcagat tcggcatgat agctcgggcg ataatcggcg
      301 ttgaatgcat gcccggcatc cgggtagacg ataatctctg ctttcgcatt agcagcccgc
      361 agcgcctggc gcatggtttc aacgctctcc tgcggaatgc tgttatcctg accaccatat
      421 aagccgagaa ccggcgcgtt aagatcggtt gcgatatcaa caggttgttt cggtgaattc
      481 agcgacttgt cgcccgtcag tttgccgtac cacgccactg cggcttttag ctgtggatta
      541 tgcgcggcat acagccaggt gatacgtcca ccccagcaga atccggtgat cattaaacga
      601 tgaacatcgc cgccgttgcg ggaagcccaa ctggcgacat gatcgagatc ggccagcacc
      661 tgcgagtcag gcacttttgc taccagaccg ctaagcaacg tggggatatc ggcaaaatca
      721 ttcggatcgc cttcgcggaa gtaaagttca ggtgcaatag ccagataccc ctccagcgcc
      781 agacggcgac aaatgtcgcg gatatgttca tgcacgccaa aaatttcctg cactacaatg
      841 accactggca gtgggccatc gctttgcttt ggtctggcat ggtaagcagg catgttatcc
      901 ccttgtgaag ggatagaggt gaagcccgcc acaatcgcgt cgtccggagt ctgaacgatg
      961 gtcgaagcga gaggcgatgc cgcaggtgca aatccagatt gttgtgttgt tgccatggta
     1021 ttctccgtac ccttataaaa atgttgcgca atgttaacta tagtcagcat gcaacaaatc
     1081 acattgcctg aatcggctca tcttttatgc agtcctgcag aatgaagagt gatttatgtg
     1141 atttgcatca cttttgatgg gtaaatttat gcaacgcatt tgcgtcatgg tgatgagtat
     1201 cacgaaaaaa tgttaaaccc ttcggtaaag tgtctttttg cttcttctga ctaaaccgat
     1261 tcacagagga gttgtatatg tccaagtctg atgtttttca tctcggcctc actaaaaacg
     1321 atttacaagg ggctacgctt gccatcgtcc ctggcgaccc ggatcgtgtg gaaaagatcg
     1381 ccgcgctgat ggataagccg gttaagctgg catctcaccg cgaattcacc acctggcgtg
     1441 cagagctgga tggtaaacct gttatcgtct gctctaccgg tatcggcggc ccgtctacct
     1501 ctattgctgt tgaagagctg gcacagctgg gcattcgcac cttcctgcgt atcggtacaa
     1561 cgggcgctat tcagccgcat attaatgtgg gtgatgtcct ggttaccacg gcgtctgttc
     1621 gtctggacgg cgcgagcctg cacttcgcac cgctggaatt cccggctgtc gctgatttcg
     1681 aatgtacgac tgcgctggtt gaagcggcga agtcaattgg tgccaccact cacgttggtg
     1741 tgacggcttc ttctgatacc ttctacccgg gtcaggaacg ttacgatact tactctggtc
     1801 gcgtagttcg tcactttaaa ggttctatgg aagagtggca ggcgatgggc gtaatgaact
     1861 atgaaatgga atctgcaacc ctgctgacca tgtgtgcaag tcagggcctg cgtgccggta
     1921 tggtagcggg ggttatcgtt aaccgcaccc agcaagagat cccgaatgct gagacgatga
     1981 aacaaaccga gagccatgcg gtgaaaatcg tggtggaagc ggcgcgtcgt ctgctgaaat
     2041 aaaaaacgct ccccaacttc ggggagcgat atcagttact gacgccgtaa acgcattatt
     2101 ttgcggatta ttggcgtcag tagcgtaaag gctgccagag ttagcaatgc cagagtgatc
     2161 ggtcgggtat acagaaagtc cagtgaaccc cctgaaagcg acagcgcacg gcgaaaatta
     2221 ctttctgcca tcgggcccat aatcaatgcc agcactacgg gtgatgccgg atatcctcct
     2281 ttctgcatga aatagcccac cacgcctgca atcagcataa tgccaacatc aaacaggcta
     2341 ttgttcaacg cataagtccc gaccacacaa agtgcgagga tcatcggtgt gagaatattg
     2401 ccggggatct tcaccacgtt accgatgact ttcaaagaga gcagtccaag aactagcagc
     2461 atgaagtaac aaaagatcat gccgacaaac agtgtataaa ccatatcgcc gtggtcggta
     2521 aacatcagcg ggcctggttg cattccctgt aacgttaacg cgcccagcat gatggcggtt
     2581 accgcatcgc cagggatccc cagcgtcagc ataggtaaca atgcgccacc ggtgcagcca
     2641 ttcgcgcctg cctcacagga ggccacagct ttaatttcac ccttaccaaa gttttctggt
     2701 gtcttactga aacgacgagt ttcgttatag gcaacaaaag ctgcaatatc cgcccccgca
     2761 ccggggatca tgccgataaa aacccctaat cctgatgagc gcaaaatggt taatgcaatg
     2821 cggcgtagtg tttgccaggg cagtaacaga ctgccgatag ccaccttcgc cccctgacga
     2881 atttgctggt tttgctccat agagcggaat gcttctgaag cagcaaataa accaatcatc
     2941 accgggataa atggcacatt catcagctcc acaaatccac ctgtaaaccg tggaaaaccg
     3001 cccattggat caaggcctat cgttgcgatc agcaatccgc caaccccggc aatcagccct
     3061 ttaatcagcg actcaccgga aatactggca ataatactca gtccgaaggt tgccagggcg
     3121 aaggactccg aggcactaaa tttcagcgcc caactggcaa gtaccggaga gagaaacatc
     3181 agcacgataa tgctgagcgt tccgccacta aaggaagaga acgtggcggc actcaatgcc
     3241 agccctgctt ttccctgttt cgtcagctca taaccatcaa tagcggtggc tgcggcggca
     3301 ggcgttccgg gaattttgag caaaattgcg gtgatagaac caccgtagac gccaccaaaa
     3361 aagacgccgc aaatcatcaa caagccagaa acaggctcca tgccgtaggt gaaaggcagc
     3421 aaaatcgcca cacccatggt cgctgtcaga ccaggcaaag cgccaatcac aatacccagc
     3481 atcaccccaa aagtggcaaa cagcagcgcc tgaggattat tgagtaatgt cgaaaaaccc
     3541 tgagttaaaa gttcactttc aaacattgct ggcccctgtt attcgaaaaa tgtgccgaca
     3601 ggcaatggca cctgtaacgc ttcgacaaac agcaaccaga ggattgccgt taacagcagc
     3661 gcaatacccg gtgtgagtac ccatcgcctg tagcccatca acgtcataat gacgatgagg
     3721 aaaacaggag tagtaatgag ataaccgcag tagctcatcg ccacaatata aaacgcggtt
     3781 gccgcaattc ctgttgctgt tttcctgaat gccggttttt cctgtgcgtt ggcgcaagag
     3841 gctggttttg cctgcaaaag atcgcgtatc accagcaaga cagcgagcac aatcagggca
     3901 caggcataga aggtagggaa ggcacttgca ccgggatccg tttcaatggc agttgctgga
     3961 aaatcagcgg cattaatcat caccgcgcca gcaaccacac aaagtaacag cgccaccacc
     4021 atcgttgatg aaaggcgctt gcggtgcggt acggcgagtt gagcaacaga ctgttttgtg
     4081 ttcatcaacc gtacctcagg tttatttttt caggcccagg cgagtcagca actcgtcaaa
     4141 gtacttttcc tgttcgctga tttgggtctg gaagctggca gcgtcaagcc atgcatagtt
     4201 gagattcaac ttttgcagtg catcctggaa tgcaggctct tcagccgtct cttttgctgc
     4261 ctttgccaga acatccacca catcctgcgg cgttttttgc gacacaatca ggccgcgcca
     4321 ggtgccgatg gagagatcaa cgcctttctc ttttaacgtc gggacgtcag gcatggtttt
     4381 cattcgctca tccgccatca ctaccagtgt cttcagcttg ccgccattca catggttgat
     4441 aacttctcct gggcttacgg aaaccgcttc aatatgcccg cctaacaggc ctgtaatggc
     4501 aggggctgcg ccgtcatacg ggacatgaga aaactttgtg cccgttttgt cttccagtgc
     4561 agctaccgcc agatgccaga tagctccggt gcctgagtta ccaatgcgta cttttccggg
     4621 attcgctttg gagtaagcca taaattcttc atagctattc cacggcgcat cggcacggac
     4681 tgtgatagca gccggatccg cattcagacg ggcaatgggt ttaaagtcgc tggttttaaa
     4741 acgcaccatt ccgaggctgg gaagggtggt cagttcaacc gtgcctaaac caattttgta
     4801 accgttaggg cgggcagcgg cgatttcact caggccgata gcaccgcctc cgccaggttt
     4861 attgataacc ccgatgctga cgggtaaatg gtttttggcg gcatcagcaa aggcacgggc
     4921 aaccagatcc gtaccgcctc cggcagcgta gggaacgact aactcgatct gtttggttgg
     4981 gtaggaggca gcctgtacag gcgcaatgct gagggcagcg cctgcaagaa gtaccattgc
     5041 agccattttt ttcggtaagg taagatgttt tatcattgct tttatatcct tatttatcag
     5101 ttagttgcta tttaaattgt tccatgcgtt cataaacagt actgcatttt catagacttg
     5161 ctctggcgtc atgccggggc gatatagcgc agaaccgagg ccaaagccgt ttgcgccgtg
     5221 gctcagataa cgcgccatgg tttccggtgt gataccgcca accggtaaca tcggaacgga
     5281 ttgtggaata accgcacgcc aggctttggt aacttctggc gtaatttgtt cagccgggaa
     5341 cagcttgatg gcattagcac catgttcaat ggcagcaaag gcttcggtgg gcgtggcgac
     5401 gccaggcgca caatacatac ccagcgcacg ggcgcgacgg atcaccgcag tatcgcaatg
     5461 cggcatcacg ataatctggc ccccagcctc tttaaccgca accacctgct ctaccgaaag
     5521 cacagttccg gctccgacca ggcagtcctt aggcagggcg tcgcgcagta aactgatact
     5581 ttgcaacgcc tccggcgagt tcataggaat ctcaatcagg cggaatccgg cggcgtacag
     5641 tttttcaccg atggcgacaa tctcatccgg tttcacgccg cgtaaaatgg cgactaaccc
     5701 ggttttttgc aaaagtgtat taaatggcat gggtacattc cctggcgttg gtggacagta
     5761 acccggcagc gtgggcgatt cgccataatc catgttcagt ggcattagga atggcgtgta
     5821 tctgtctgtc gggaaaaacg tggctgaatg cttgtgaata gcggctgttc agatttgcgc
     5881 taccgattaa ggcgatcggt gttgtggcgg gtaaatcacg tagcaaactg gatgccagac
     5941 cacataattc atggcctatc agtaatccgg aaaggtaatc cccctgcgag gacgacgtaa
     6001 gattactggt cagcaggcgc gaacgggtcg aaaacagcgt cgataataat ccggtctgat
     6061 gatgctcttg tgctgctgtc agcccgtggg tgaaggcatc ccagtgcggt tcatctccag
     6121 gatgcatggt ggcaccgaga atcgaatggc gggataaaac atcaaataac tcgcccgtca
     6181 taaaggttcg gaaatcggta atggtgttgt tttcaactac tgcccatttc gcatgtgtgc
     6241 cgggtaagcc tatcagcact ggcataccct gatggatcgc gttttgcagg gccggttcca
     6301 tcgagatagc accgaagatt tgtgtctctt cgccacgcat cacttcgggc atttcacctt
     6361 ctttaatcac gccaggaatg atgtgcagca ttgtctgctg ctggtggata acaggcgtca
     6421 actgcttcgc cagaccctcg agagtgaccg ggcaaggtaa ataagctgct gattgccacc
     6481 cttgcgcact gccgagcatg ccgcaagcca gtaccgggca tggggtttgt gccagccagt
     6541 cgccgcagac acgtaaaaac gtatcgtgga aagcgtcctc acgtgttgct gcctgggagg
     6601 gtaatttcat gatcccccag gggaattcac gtttttcttg tggatttggt gattcgccaa
     6661 aacgccatgc ccgaagcgat gacgttcccc agtccagggc aataaatgag ggtgctcccg
     6721 gcattagtcg tttcctttaa tcatggttta tttatctttg cggacgtgct taccccagcc
     6781 cagttcagcg gatatctctt ctgcacagga tttgacgtaa ggtgccagtt cttccagacg
     6841 cgccagcgac atatacgtgg tggtactggc gacagaaata gccgccacaa tttcatcccg
     6901 ggcgttgtaa accggcgctg caacgcaacg gatagtcggc tcgttctctt ccagatcaaa
     6961 agcaaaacca ctggcggcat aacgacgcat gttctgaata aatgcgccca gttttgtttc
     7021 atcaccacag gtcttaaaca gcgtgcgcca ctcttcttct gttcggttga ggatcaatgc
     7081 cttgccgatg ccggtgattg ccagtggcat gcgatggcct gggcgtgagc gcatttccag
     7141 cccgcgctga ctgttgatct tctccagata cagcacttcg tcgccttcaa cgatccccag
     7201 atgcacggtg tccagggtgt aacgggcaag gcgttgcaaa acgggctggg caatttcgta
     7261 cagtgaggtg ctttccagtg ctctggcacc gaattcgatc agtttggcac ccagttggta
     7321 gccctgcgtt gggacatggc gcaggtagcg ttgttccacc agaacggtga ctaaacgatg
     7381 ggttgtgctc cgggtcgtgc cggtgaactc gccgatactt tttaggtcat ggcagccgtt
     7441 ataaacagca ttaagaaccg ccagtccacg taataacgtt tgtgcgcctg cggtggttga
     7501 agaggtttct gccatacgtt tctcctgatg atattgctga agtgttccac ataatgacgt
     7561 ggggaaaagg tgatatatcc cacaatataa actccatctc atattgtggg ataaaataat
     7621 gaaaattaac gatcgttttg cgtagcgcct cgcaggaaac ttctttcaaa ctggacgttt
     7681 gtacagcaca attctatttt gtgcgggtaa gttgttgggt caggaggctt tgtggatttc
     7741 tcaatcattg tttatgccat tattgcgctg gttagtgtgg tcattggctg gctgtttgcc
     7801 agttatcaac atgcgcagca aaaagccgag caattagctg aacgtgaaga gatggtcgcg
     7861 gagttaagcg cggcaaaaca acaaattact caaagcgagc actggcgcgc tgagtgcgag
     7921 ttactcaata acgaagtgcg cagcctgcaa agtattaaca cctctctgga ggccgatctg
     7981 cgtgaagtaa ccacgcggat ggaagccgca cagcaacatg ctgacgataa aattcgccag
     8041 atgatcaaca gcgagcagcg cctcagtgag cagtttgaaa acctcgccaa ccgtattttt
     8101 gagcacagca atcgccgggt tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg
     8161 ccgctacgtg aacaactgga cggtttccgc cgtcaggttc aggacagctt cggtaaagaa
     8221 gcacaggaac gtcataccct gacccacgaa attcgcaatc tccagcagct caacgcgcaa
     8281 atggctcagg aggcgatcaa cctgacgcgc gcgctgaaag gcgacaataa aacccagggc
     8341 aactggggcg aggtggtatt gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat
     8401 gaatatgaaa ctcaggtcag catcgaaaat gacgcccgct cgcggatgca gccggatgtc
     8461 atcgtgcggt tgccgcaggg aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc
     8521 tatgaacgct atttcaacgc cgaagatgac tacacccgcg aaagcgcgct acaggaacat
     8581 atcgcgtcgg tgcgtaacca tatccgtttg ctgggacgca aagattatca acagctgccg
     8641 gggctgcgaa ccctggatta cgtgctgatg tttattcccg ttgaacccgc ttttttactg
     8701 gcgcttgacc gccagccgga gctgatcacc gaagcgttga aaaacaatat catgctggtt
     8761 agcccgacta cgctgctggt ggcgctgcgc actatcgcca acctgtggcg ttatgagcat
     8821 caaagccgca acgcccagca aatcgccgat cgtgccagca agctgtacga caagatgcgt
     8881 ttgttcgtcg atgacatgtc agcgattggt caaagtctcg acaaagcgca ggataactac
     8941 cgccaggcaa tgaaaaaact ctcttcaggt cgcggaaatg tgctggcgca ggcagaagcg
     9001 tttcgcggtt taggggtaga aattaaacgc gagattaatc cggatttggc tgaacaggcg
     9061 gtgagccagg atgaagagta tcgacttcgg tcggttccgg agcagccgaa tgatgaagct
     9121 tatcaacgcg atgatgaata taatcagcag tcgcgctagc ccattggggg tagttaagcc
     9181 gggtagaaat ctagggcatc gacgcccaat ctgttacact tctggaacaa ttttttgatg
     9241 agcaggcatt gagatggtgg ataagtcaca agaaacgacg cactttggtt ttcagaccgt
     9301 cgcgaaggaa caaaaagcgg atatggtcgc ccacgttttc cattccgtgg catcaaaata
     9361 cgatgtcatg aatgatttga tgtcattcgg tattcatcgt ttgtggaagc gattcacgat
     9421 tgattgcagc ggcgtacgcc gtgggcagac cgtgctggat ctggctggtg gcaccggcga
     9481 cctgacagcg aaattctctc gcctggtcgg agaaactggc aaagtggtcc ttgctgatat
     9541 caatgaatcc atgctcaaaa tgggccgcga gaagctgcgt aatatcggtg tgattggcaa
     9601 cgttgagtat gttcaggcga acgctgaggc gctgccgttc ccggataaca cctttgattg
     9661 catcaccatt tcgtttggtc tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat
     9721 gtatcgcgtg ctgaaacccg gcggccgcct gctggtgctt gagttctcga agccaattat
     9781 cgagccgctg agcaaagcct atgatgcata ctccttccat gtgctgccgc gtattggctc
     9841 actggtcgcg aacgacgccg acagctaccg ttatctggca gaatccatcc gtatgcatcc
     9901 cgatcaggat accctgaaaa ccatgatgca ggatgccgga ttcgaaagtg tcgactacta
     9961 caatctgacg gcaggggttg tggcgctgca tcgtggttat aagttctgac aggagaccgg
    10021 aaatgccttt taaaccttta gtgacggcag gaattgaaag tctgctcaac accttcctgt
    10081 atcgctcacc cgcgctgaaa acggcccgct cgcgtctgtt gggcaaagtg ctgcgcgtgg
    10141 aggtaaaagg cttttcgacg tcattgattc tggtgttcag cgaacgccag gttgatgtac
    10201 tgggcgaatg ggcaggcgat gctgactgca ccgttatcgc ctacgccagt gtgttgccga
    10261 aacttcgcga tcgccagcag cttgccgcac tgattcgcag tggtgagctg gaagtgcagg
    10321 gcgatattca ggtggtgcaa aacttcgttg cgctggcaga tctggctgag ttcgaccctg
    10381 cggaactgct ggccccttat accggtgata tcgccgctga aggtatcagc aaagccctgc
    10441 gcgggggcgc aaagttcctg catcacggca ttaagcgcca gcaacgttat gtggcggaag
    10501 ccattactga agagtggcgt atggcacccg gtccgcttga agtggcctgg tttgcggaag
    10561 agacggctgc cgtcgagcgt gctgttgatg ccctgaccaa acggctggaa aaactggagg
    10621 ctaaatgacg ccaggtgaag tacggcgcct atatttcatc attcgcactt ttttaagcta
    10681 cggacttgat gaactgatcc ccaaaatgcg tatcaccctg ccgctacggc tatggcgata
    10741 ctcattattc tggatgccaa atcggcataa agacaaacct ttaggtgagc gactacgact
    10801 ggccctgcaa gaactgggac cggtatggat caagttcggg cagatgttat caacccgccg
    10861 cgatcttttt ccgccgcata ttgccgatca gctggcgtta ttgcaggaca aagtcgctcc
    10921 gtttgatggc aagctggcga agcagcagat tgaagctgca atgggcggct tgccggtaga
    10981 agcgtggttt gacgattttg aaatcaagcc gctggcttct gcttctatcg cccaggttca
    11041 taccgcgcga ttgaaatcga atggtaaaga ggtggtgatt aaagtcatcc gcccggatat
    11101 tttgccggtc attaaagcgg atctgaaact tatctaccgt ctggctcgct gggtgccgcg
    11161 tttgctgccg gatggtcgcc gtctgcgccc aaccgaagtg gtgcgcgagt acgaaaagac
    11221 cttgattgat gaactgaatt tgctgcggga atctgccaac gccattcagc ttcgacgcaa
    11281 ttttgaagac agcccgatgc tctacatccc ggaagtttac cctgactatt gtagtgaagg
    11341 gatgatggtg atggagcgta tttacggcat tccggtgtct gatgttgcga cgctggagaa
    11401 aaacggcacc aacatgaaat tgctggcgga acgcggcgtg caggtgttct tcactcaggt
    11461 ctttcgcgac agctttttcc atgccgatat gcaccctggc aacatctttg taagctatga
    11521 acacccagaa aacccgaaat atatcggcat tgattgcggg attgttggct cgctaaacaa
    11581 agaagataaa cgctatctgg cggaaaactt tatcgctttc tttaatcgcg attatcgcaa
    11641 agttgcagag ctacacgtcg attcaggctg ggtgccgcca gataccaacg ttgaagagtt
    11701 cgaatttgcc attcgtacgg tctgtgaacc tatctttgag aaaccgttgg ccgaaatttc
    11761 gtttggacat gtgctgttaa atctatttaa tacggcgcgt cgcttcaata tggaagtgca
    11821 gccgcaactg gtgttactcc agaaaaccct gctctacgtc gaaggggtag gacgccagct
    11881 ttatccgcag ctcgatttat ggaaaacggc gaagcctttc cttgagtcgt ggattaaaga
    11941 tcaggtcggt attcctgcgc tggtgagagc atttaaagaa aaagcgccgt tctgggtcga
    12001 aaaaatgcca gaactgcctg aactggttta cgacagtttg cgccagggca agtatttaca
    12061 gcacagtgtt gataagattg cccgcgagct tcagtcaaat catgtacgtc agggacaatc
    12121 gcgttatttt ctcggaattg gcgctacgtt agtattaagt ggcacattct tgttggtcag
    12181 ccgacctgaa tgggggctga tgcccggctg gttaatggca ggtggtctga tcgcctggtt
    12241 tgtcggttgg cgcaaaacac gctgattttt tcatcgctca aggcgggccg tgtaacgtat
    12301 aatgcggcct tgtttaatca tcatctacca cagaggaaca tgtatgggtg gtatcagtat
    12361 ttggcagtta ttgattattg ccgtcatcgt tgtactgctt tttggcacca aaaagctcgg
    12421 ctccatcggt tccgatcttg gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga
    12481 tgaaccaaag caggataaaa ccagtcagga tgctgatttt actgcgaaaa ctatcgccga
    12541 taagcaggcg gatacgaatc aggaacaggc taaaacagaa gacgcgaagc gccacgataa
    12601 agagcaggtg taatccgtgt ttgatatcgg ttttagcgaa ctgctattgg tattcatcat
    12661 cggcctcgtc gttctggggc cgcaacgact gcctgtggcg gtaaaaacgg tcgcgggctg
    12721 gattcgcgcg ttgcgttcac tggcgacaac ggtgcagaac gaactgaccc aggagttaaa
    12781 actccaggag tttcaggaca gtctgaaaaa ggttgaaaag gcgagcctca ctaacctgac
    12841 gcccgaactg aaagcgtcga tggatgaact acgccaggcc gcggagtcga tgaagcgttc
    12901 ctacgttgca aacgatcctg aaaaggcgag cgatgaagcg cacaccatcc acaacccggt
    12961 ggtgaaagac aatgaagctg cgcatgaagg cgtaacgcct gctgctgcac aaacgcaagc
    13021 cagttcgccg gaacagaagc cagaaaccac gccagagccg gtggtaaaac ctgctgcgga
    13081 cgctgaaccg aaaaccgctg caccttcccc ttcgtcgagt gataaaccgt aaacatgtct
    13141 gtagaagata ctcaaccgct tatcacgcat ctgattgagc tgcgtaagcg tctgctgaac
    13201 tgcattatcg cggtgatcgt gatattcctg tgtctggtct atttcgccaa tgacatctat
    13261 cacctggtat ccgcgccatt gatcaagcag ttgccgcaag gttcaacgat gatcgccacc
    13321 gacgtggcct cgccgttctt tacgccgatc aagctgacct ttatggtgtc gctgattctg
    13381 tcagcgccgg tgattctcta tcaggtgtgg gcgtttatcg ccccagcgct gtataagcat
    13441 gaacgtcgcc tggtggtgcc gctgctggtt tccagctctc tgttgtttta tatcggcatg
    13501 gcgttcgcct actttgtggt ctttccgctg gcatttggct tccttgccaa taccgcgccg
    13561 gaaggggtac aggtatccac cgacatcgcc agctatttaa gcttcgttat ggcgctgttt
    13621 atggcgtttg gtgtctcctt tgaagtgccg gtggcaatag tgctgctgtg ctggatggga
    13681 attacctcgc cagaagactt acgcaaaaaa cgtccgtatg tgctggttgg cgcatttgtt
    13741 gtcgggatgc tgctgacgcc gccggatgtc ttctcgcaaa cgctgctggc gatcccgatg
    13801 tattgcctgt ttgaaatcgg tgtcttcttc tcacgctttt acgttggtaa agggcgaaat
    13861 cgggaagagg aaaacgacgc tgaagcagaa agcgaaaaaa ctgaagaata aattcaaccg
    13921 cccgtcaggg cggttgtcat atggagtaca ggatgtttga tatcggcgtt aatttgacca
    13981 gttcgcaatt tgcgaaagac cgtgatgatg ttgtagcgcg cgcttttgac gcgggagtta
    14041 atgggctact catcaccggc actaacctgc gtgaaagcca gcaggcgcaa aagctggcgc
    14101 gtcagtattc gtcctgttgg tcaacggcgg gcgtacatcc tcacgacagc agccagtggc
    14161 aagctgcgac tgaagaagcg attattgagc tggccgcgca gccagaagtg gtggcgattg
    14221 gtgaatgtgg tctcgacttt aaccgcaact tttcgacgcc ggaagaacag gaacgcgctt
    14281 ttgttgccca gctacgcatt gccgcagaat taaacatgcc ggtatttatg cactgtcgcg
    14341 atgcccacga gcggtttatg acattgctgg agccgtggct ggataaagtg cctggtgcgg
    14401 ttcttcattg ctttaccggc acacgcgaag agatgcaggc gtgcgtggcg catggaattt
    14461 atatcggcat taccggttgg gtttgcgatg aacgacgcgg gctggagctg cgggaattgt
    14521 tgccgttgat tccggcggag aaattgctga tcgaaactga tgcgccgtat ctgctccctc
    14581 gcgatctcac gccaaagcca tcatcccggc gcaacgagcc agcccatctg ccccatattt
    14641 tgcaacgtat tgcgcactgg cgtggagaag atgccgcatg gctggctgcc accacggatg
    14701 ctaatgtcaa aacactgttt gggattgcgt tttagagttt gcggaactcg gtgttcttca
    14761 cactgtgctt aatctcttta ttaataagat tcagcaatag catggagcga gcctcgccgt
    14821 ctggctcggt gaaaatggcc tgaaaacctt cgaatgcgcc ttcagtaata atcactttat
    14881 caccaggata cggcgtcgat ggatcgacaa tatctttcgg tttatacact gataactgat
    14941 gaatcaccgc cgatgggact atcgctggcg acgcgccaaa gcgcacgaag tggctgacac
    15001 cgcgggtcgc gttgatagtc gtggtatgaa tcacttccgg atcgaactcc acaaacaagt
    15061 agttggggaa caatggctca ctgactgcag tacgtttgcc acgcacgatt ttttccaggg
    15121 tgatcatcgg tgccaggcaa ttcacagcct gtctttcgag gtgttcctgg gcacgttgaa
    15181 gttgcccgcg cttgcagtac agtaaatacc aggattgcat aatgactctt atccgtttaa
    15241 tcggggcgca aggatagcaa aagctttacg ctaagttaat tatattcccc ggtttgcgtt
    15301 ataccgtcag agttcacgct aatttaacaa atttacagca tcgcaaagat gaacgccgta
    15361 taatgggcgc agattaagag gctacaatgg acgccatgaa atataacgat ttacgcgact
    15421 tcttgacgct gcttgaacag cagggtgagc taaaacgtat cacgctcccg gtggatccgc
    15481 acctggaaat cactgaaatt gctgaccgca ctttgcgtgc cggtgggcct gcgctgttgt
    15541 tcgaaaaccc taaaggctac tcaatgccgg tgctgtgcaa cctgttcggt acgccaaagc
    15601 gcgtggcgat gggcatgggg caggaagatg tttcggcgct gcgtgaagtt ggtaaattat
    15661 tggcgtttct gaaagagccg gagccgccaa aaggtttccg cgacctgttt gataaactgc
    15721 cgcagtttaa gcaagtattg aacatgccga caaagcggct gcgtggtgcg ccttgccaac
    15781 aaaaaatcgt ctctggcgat gacgtcgatc tcaatcgtat tcccattatg acctgctggc
    15841 cggaagatgc cgcgccgctg attacctggg ggctcaccgt aacgcgcggc ccgcataaag
    15901 agcggcagaa tctgggcatt tatcgccagc agctaattgg taaaaacaaa ctgattatgc
    15961 gctggctgtc gcatcgcggc ggcgcgttgg attatcagga gtggtgtgcg gcgcatccgg
    16021 gcgaacgttt cccggtttct gtggcgctgg gtgccgatcc cgccacgatt ctcggtgcag
    16081 tcacccccgt tccggatacg ctttcagagt atgcgtttgc cggattgctg cgcggcacca
    16141 agaccgaagt ggtgaagtgt atctccaatg accttgaagt gcccgccagt gcggagattg
    16201 tgctggaagg gtatatcgaa caaggcgaaa ctgcgccgga agggccgtat ggcgaccaca
    16261 ccggttacta taacgaagtc gatagttttc cggtatttac cgtgacgcat attacccagc
    16321 gtgaagatgc gatttatcat tccacctata ccgggcgtcc gccagatgaa cctgcggtac
    16381 tgggtgtagc actgaacgaa gtgttcgtgc cgattctgca aaaacagttc ccggaaattg
    16441 tcgattttta tctgccgccg gaaggctgtt cttatcgtct ggcggtagtg acgatcaaaa
    16501 aacagtatgc cggacacgcg aagcgcgtca tgatgggcgt ctggtcgttc ttacgccagt
    16561 ttatgtacac taaatttgtg atcgtttgcg atgatgacgt caacgcccgc gactggaacg
    16621 atgtgatttg ggcgattacc acccgtatgg acccggcgcg ggataccgtt ctggtagaaa
    16681 atacgcctat tgattatctg gattttgcct cccctgtctc cgggctgggt tcaaaaatgg
    16741 ggctggatgc cacgaataaa tggccggggg aaacccagcg tgaatgggga cgtcccatca
    16801 aaaaagatcc agatgttgtc gcgcatattg acgccatttg ggatgaactg gctattttta
    16861 acaacggtaa aagcgcctga tgcgcgtttg ttttgcccta tttatcgatc cgacagagaa
    16921 agcgcatgac aaccttaagc tgtaaagtga cctcggtaga agctatcacg gataccgtat
    16981 atcgtgtccg catcgtgcca gacgcggcct tttcttttcg tgctggtcag tatttgatgg
    17041 tagtgatgga tgagcgcgac aaacgtccgt tctcaatggc ttcgacgcca gatgaaaaag
    17101 ggtttatcga gctgcatatt ggcgcttctg aaatcaacct ttacgcgaaa gcagtcatgg
    17161 accgcatcct caaagatcat caaatcgtgg tcgatattcc ccacggagaa gcgtggctgc
    17221 gcgatgatga agagcgtccg atgattttga ttgcgggcgg caccgggttc tcttatgccc
    17281 gctcgatttt gctgacagcg ttggcgcgta acccaaaccg tgatatcacc atttactggg
    17341 gcgggcgtga agagcagcat ctgtatgatc tctgcgagct tgaggcgctt tcgttgaagc
    17401 atcctggtct acaagtggtg ccggtggttg aacaaccgga agcgggctgg cgtgggcgta
    17461 ctggcaccgt gttaacggcg gtattgcagg atcatggtac gctggcagag catgatatct
    17521 atattgccgg acgttttgag atggcgaaaa ttgcccgtga cctgttttgc agcgagcgta
    17581 atgcgcggga agatcgcctg tttggcgatg cgtttgcatt tatctgagat ataaaaaacc
    17641 cgcccctgac aggcgggaag aacggcaact aaaactttat acagtggcat ttagatctat
    17701 gtcgcatccg gcattcggca cacgctgcct gatgcgacgc tggcgcgtct tatcaggcct
    17761 acaattggtg caggatcggt agaccggata aggcgttcac gccgcatctg gcaagtggtt
    17821 aaatccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgcaagac
    17881 caaactgggc atccttgcgc tccatgagat ttagcagcgt gctgctgata cgcgcaccgg
    17941 aacagcccaa cggatgaccc agcgcgatcg cgccaccgtt gaggttgatc ttctcgtcaa
    18001 tctgctcaat cagtcccaga tctttaatac atggcaggat ctgcgcggca aaggcttcat
    18061 tcatttcaaa cacgccgata tcactgacag aaagccccgc ttttttcagc gccaattttg
    18121 aggccggaac cggaccgtaa cccataatcg acggatcaca accaacgacc gccatcgaac
    18181 gcacacgggc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    18241 tggcagctgc gccatcggaa agcgcagaag atgtacccgc cgttaccgta ccgctgacgg
    18301 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    18361 cgtcgtaatt aaactgcgtc aggacgccgt cggcatcgtg accaccggtc gggataattt
    18421 catttttaaa tgcacccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    18481 aggcatcctg catttcacgg ctgataccgt gcatacgcgc cagcatttct gccgttaagc
    18541 ccatcatgcc cgccgctttg gcgacattac ggctaaggcc ggggtgaaaa tcgacgccgt
    18601 gactcattgg cacatggccc atatgctcca cgccgccaac cagacatgtc tgcgcatcgc
    18661 cgatcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    18721 tgacagtaac cgccgggaca gagtgcggca cttctgccaa cagcgccgcg ttacgggcga
    18781 tattaaaacc ctgctccagc gtctgctgta cacaacccca gtaaatatcg tcgagggccg
    18841 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    18901 ccgcacgcac gtgacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    18961 cgacaatgac aacctgttcc attgtgactc cttaagccat tttcaggtcg ccaaccggac
    19021 gggctggctc aaccggggga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    19081 ccggcacttc atacagcggg ccgagatgct gatattgctg tgccatatcg aggtattttg
    19141 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg aaacggaggg aagcccaggc
    19201 cgtagaccag cgccatatcc gcttctgccg gagtggcgat aatgccttct tccagacagc
    19261 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    19321 cgcgcttcgg ctggctcact tctgccagca ggtcgtcaac gacggcatct tcttctttct
    19381 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    19441 agcggttggc attaaacagc gcgtcgatgg catcgcgata atctttctgc atccgctgcg
    19501 ggaagcctgc tgccatgaca gcctgggcgt gatgcgcggt gtcaatgccc acaacatcca
    19561 gcagatatgc cggacccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    19621 ggaaatccgc gccgtcgcgc agcaactggc tgaaaccggc gaaatagggg aacagtacac
    19681 ggttgacgaa gaagccgggg cagtcgttaa ccacgatcgg cgtcttgccc atcttgctcg
    19741 cccatgcgac gactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcca
    19801 ccaacggcat ccggtgcacc gggttaaaga agtgcatccc gcagaagttt tccggacgtt
    19861 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    19921 ccgggcgtac tttctgctcg gtttctgcca gtacggcttt tttcactttc gggttttcga
    19981 caaccgcttc taccacaaca tccacgcggt caaaaccaga gtaatcgagc gttgggtgga
    20041 ttgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    20101 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    20161 tgacaaccgg tacgcctttc cacgcagact ggtaagcgat gccgccaccc ataatgcctg
    20221 cgcccagcac agcggcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    20281 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    20341 ccagcgggac aaaacttttg ttttccagat ttaatgcttc ttcacgacca aaacgggccg
    20401 ccgcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgtg
    20461 cgaccatccc tttagcgatg gtgaaactca tggtggcttc aatcttgctc agttttagcg
    20521 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    20581 aaatcgccat cgccccttca accagttttt ctgctttgac tacaccatcc accagaccga
    20641 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc aatgatttcc agcgcactgt
    20701 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    20761 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    20821 cgcattcgca gccaccgccc agcgcatagc cgttgacggc agcaatggtc ggcaccggga
    20881 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    20941 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    21001 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    21061 cgccgaggct ggcgacggtg gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    21121 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    21181 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcga agtgtgtatt
    21241 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    21301 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    21361 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacagga
    21421 acggactcgc gatgcgctgg cgcgcttcaa gctggatgcg ttacttattc actccggcga
    21481 actgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    21541 agcgtgggtg ccagtaactc aggtgccaaa ctgctggctg ctggtggatg gcgtgaataa
    21601 gccgaaactg tggttctatc taccggttga ttactggcac aacgtcgaac cgctgccgaa
    21661 ctccttctgg actgaagatg tagaagtgat cgcgctgccg aaagccgatg gcattggtag
    21721 tctgttgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccag aacgtgcgct
    21781 gcaactgggt attgaggcca gcaatatcaa cccgaaaggg gtgatcgact acctgcatta
    21841 ctatcgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    21901 ggtcaacggt catcgcgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    21961 caacattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt atagcaacat
    22021 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    22081 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    22141 tctaacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    22201 aaatgatgaa caactggcgc tgatcgctac catgaaagca ggcgtcagct atgtggatta
    22261 ccatatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    22321 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    22381 cggccatccg ctgggcctgc aggtgcatga cgtagccggt tttatgcagg atgatagcgg
    22441 tacgcacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    22501 gggcatggtg ttaaccatcg aaccgggtat ctacttcatc gaatcgcttc tggcaccgtg
    22561 gcgtgaaggg cagttcagca aacacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    22621 cggcggcatt cgtatcgaag acaacgtggt gattcacgaa aacaacgtgg aaaacattac
    22681 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    22741 gttgaagaga tcaaaaagag ccgcttcatt acgctgttgg cgcataccga tggcgttgag
    22801 gcggcaaaag cgtttgttga atcggtgcgg gcagagcacc ccgatgcccg ccaccattgc
    22861 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    22921 gagccggcgg gaacggcagg taaaccgatg ctcgcccaac taatgggcag cggcgtcggg
    22981 gaaattaccg ctgtggtggt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    23041 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    23101 acaccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    23161 ttgctggggc agtgtgacgg caaaattatt aacagtgatt atcaggcatt cgttctgctg
    23221 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    23281 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    23341 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    23401 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    23461 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    23521 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    23581 tctggaccgt gctgggtagc gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    23641 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacg ggggccacta
    23701 cgctagtggg gctggattcg ctccctcacg ccatcctctt ttatcgccag atgctgcaat
    23761 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    23821 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    23881 cgcgaattgc ggaaacggcg aaaaccctgt ggttgattta tgtcttgctg accgtcgcct
    23941 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    24001 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagcc
    24061 cgactattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    24121 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    24181 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    24241 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    24301 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    24361 tgctcttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    24421 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    24481 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    24541 ccgtgtgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    24601 tcgccacggg cgtggatgac ttttctgcct ttgcctcggt tgttgcgaca ttgaataacc
    24661 tggggccagg gcttggtgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    24721 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    24781 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    24841 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    24901 ggggatccag gcggatgtcg ccaatgtgca ccgcattgaa gaaccacagt gggaaaacta
    24961 cgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    25021 gtttgtcaaa aagcatgcga cgcgactgaa ttcgatgccg agcgcctttt actccgtaaa
    25081 cctggtggcg cgtaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    25141 tctgatgagt tcacaatggc gtcccgatcg ctgtgccgtc attgccgggg cgctgcgtta
    25201 cccacgttat cgctggtacg accgttttat gatcaagctg attatgaaga tgtcaggcgg
    25261 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    25321 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa
    25381 tg
//
LOCUS       DAZUCC010000056        24850 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZUCC010000056 DAZUCC010000000
VERSION     DAZUCC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.056"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..616
                     /gene="nohD"
                     /locus_tag="ACHY09_001968"
     CDS             71..616
                     /gene="nohD"
                     /locus_tag="ACHY09_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFS3884946.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            591..2516
                     /locus_tag="ACHY09_001969"
     CDS             591..2516
                     /locus_tag="ACHY09_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFS3884947.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2513..2719
                     /locus_tag="ACHY09_001970"
     CDS             2513..2719
                     /locus_tag="ACHY09_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFS3884948.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2716..4317
                     /locus_tag="ACHY09_001971"
     CDS             2716..4317
                     /locus_tag="ACHY09_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS3884949.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4298..5617
                     /locus_tag="ACHY09_001972"
     CDS             4298..5617
                     /locus_tag="ACHY09_001972"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFS3884950.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5627..5959
                     /locus_tag="ACHY09_001973"
     CDS             5627..5959
                     /locus_tag="ACHY09_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS3884951.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6015..7040
                     /locus_tag="ACHY09_001974"
     CDS             6015..7040
                     /locus_tag="ACHY09_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS3884952.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            7082..7477
                     /locus_tag="ACHY09_001975"
     CDS             7082..7477
                     /locus_tag="ACHY09_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS3884953.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            7489..7842
                     /locus_tag="ACHY09_001976"
     CDS             7489..7842
                     /locus_tag="ACHY09_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS3884954.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7854..8432
                     /locus_tag="ACHY09_001977"
     CDS             7854..8432
                     /locus_tag="ACHY09_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3884955.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRISGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8429..8824
                     /locus_tag="ACHY09_001978"
     CDS             8429..8824
                     /locus_tag="ACHY09_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFS3884956.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8832..9572
                     /locus_tag="ACHY09_001979"
     CDS             8832..9572
                     /locus_tag="ACHY09_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3884957.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            9588..10010
                     /locus_tag="ACHY09_001980"
     CDS             9588..10010
                     /locus_tag="ACHY09_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFS3884958.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            9992..10426
                     /locus_tag="ACHY09_001981"
     CDS             9992..10426
                     /locus_tag="ACHY09_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFS3884959.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10419..12998
                     /locus_tag="ACHY09_001982"
     CDS             10419..12998
                     /locus_tag="ACHY09_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS3884960.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMAD
                     SRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSG
                     GGR"
     gene            12995..13324
                     /locus_tag="ACHY09_001983"
     CDS             12995..13324
                     /locus_tag="ACHY09_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3884961.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13324..14022
                     /locus_tag="ACHY09_001984"
     CDS             13324..14022
                     /locus_tag="ACHY09_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS3884962.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14028..14771
                     /locus_tag="ACHY09_001985"
     CDS             14028..14771
                     /locus_tag="ACHY09_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS3884963.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            14768..15340
                     /locus_tag="ACHY09_001986"
     CDS             14768..15340
                     /locus_tag="ACHY09_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS3884964.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15400..18897
                     /locus_tag="ACHY09_001987"
     CDS             15400..18897
                     /locus_tag="ACHY09_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFS3884965.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITW
                     TITSANIDRLRVTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPIWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGNP
                     PAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQNCTIKGMLEATQVRGDFVKAVS
                     KAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            18968..19567
                     /locus_tag="ACHY09_001988"
     CDS             18968..19567
                     /locus_tag="ACHY09_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFS3884966.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19632..22658
                     /locus_tag="ACHY09_001989"
     CDS             19632..22658
                     /locus_tag="ACHY09_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS3884967.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLASGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTTYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYVLMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            22655..23557
                     /locus_tag="ACHY09_001990"
     CDS             22655..23557
                     /locus_tag="ACHY09_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HFS3884968.1"
                     /translation="MITLILSAPVTEMAEAFKRVFANADNVNIVGKPFETIREFDCMV
                     SAANSFGLMDGGVDAAITTFFGSQLQARVQNHILREYLGEQPVGSAFVIETGHNYHPW
                     LVHAPTMRVPLTIDGTDAVYNATWAALLAIFQHNKNATTDRKIKTVVFPAMGAGCGQV
                     PFESVARQMKQAWDNFNKKTESINWEYAQSRQSAVFGTYAYCPGNSVCRYADTKYIGC
                     GDYRTYCSRSGQVCINPEHQADDVLIQHQANNRFRPGSHIHRINPENPVGNVISGAHS
                     HGSSIVIGAHTHTLNKQYSVSDIK"
     gene            23566..24141
                     /locus_tag="ACHY09_001991"
     CDS             23566..24141
                     /locus_tag="ACHY09_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS3884969.1"
                     /translation="MDFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(24239..24829)
                     /locus_tag="ACHY09_001992"
     CDS             complement(24239..24829)
                     /locus_tag="ACHY09_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS3884970.1"
                     /translation="MSRIYAYCRVSTLEKTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT         5883 a         6277 c         7573 g         5117 t
ORIGIN      
        1 gacaggtgct gaaagcgagc tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt
       61 ggaatgaaca atggaagtca acaaaaagca gctggctgac attttcggtg cgagtatccg
      121 taccattcag aactggcagg aacagggaat gcccgttctg cgaggcggtg gcaagggtaa
      181 tgaggtgctt tatgactctg ccgccgtcat aaaatggtat gccgaaaggg atgctgaaat
      241 tgagaacgaa aagctgcgcc gggaagttga agaactgcgg caggccagcg agacagatct
      301 ccagccaggg actattgagt acgaacgcca tcgacttacg cgtgcgcagg ccgacgcaca
      361 ggagctgaaa aatgccagag actccgctga agtggtggaa accgcattct gtactttcgt
      421 gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggatccccc tgtcggtgca
      481 gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg atatcatcaa
      541 agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga gtgaatatat
      601 cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc tcactgttca
      661 ggccggagcc acagaccgcc gttgaatggg cggatgccaa ttactatctc ccaaaagaat
      721 ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc atgaatgcga
      781 tgggcagcga ctacatccgt gaggtgaatg tggtgaagtc tgcccgtgtc ggttattcca
      841 aaatgctgct gggtgtttat gcctacttta tagagcataa gcagcgcaac acccttatct
      901 ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag ccgaccatcc
      961 gcgatattcc gtcgctgctg gcgctggctc cgtggtatgg caaaaagcac cgggataaca
     1021 cgctcactat gaagcgtttt tccaatggtc gtggcttctg gtgcctgggc ggtaaagcgg
     1081 caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta tgatgaactt gctgcctttg
     1141 atgaggatat tgaacaggaa ggctctccga cgttccttgg cgacaaacgt attgaaggct
     1201 cggtctggcc aaagtccatc cgtggctcca cgcccaaagt gagaggcacc tgccagattg
     1261 agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc ccgcactgcg
     1321 gggaggagca gtatcttaaa tttggcgaca aagagacgcc gtttggcctc aaatggacgc
     1381 cggatgaccc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc atccgccagc
     1441 aggagctgga ctttactgat gcccgttata tctgcgaaaa gaccgggatc tggacccgtg
     1501 atggcattct ctggttttcg tcatccggtg aagagattga gccgccggac agtgtgacct
     1561 ttcacatctg gacggcgtac agcccgttca ccacctgggt gcagattgtc aaagactgga
     1621 tgaagacgaa aggggatacg ggaaaacgta aaaccttcgt gaacaccacg ctcggtgaga
     1681 catgggaagc gaaaattggc gaacgtccgg atgctgaggt gatggcggag cggaaagagc
     1741 attattcagc gcccgttcct gaccgtgtgg cttacctgac cgccggtatc gactcccagc
     1801 tggaccgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc tggctgattg
     1861 accggcagat tattatgggc cgccacgacg atgaacagac gctgctgcgt gtggatgagg
     1921 ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcggtatcc cgtatctgct
     1981 gggatactgg cgggattgac ccgaccattg tgtatgaacg ctcgaaaaag catgggctgt
     2041 tccgggtgat ccccattaaa ggggcatccg tctacggaaa gccagtggcc agcatgccac
     2101 gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg tacggatacc gcgaaagagc
     2161 agatttataa ccgcttcaca ctgacgccgg aaggggatga accgcttccc ggtgccgttc
     2221 acttcccgaa taacccggat atttttgatc tgaccgaagc gcagcagctg actgctgaag
     2281 agcaggtcga aaaatgggtg gatggcagga aaaaaatact gtgggacagc aaaaagcgac
     2341 gcaatgaggc gctcgactgc ttcgtttatg cgctggcggc gctgcgcatc agtatttccc
     2401 gctggcagct ggatctcagt gcactgctgg cgagcctgca ggaagaggat ggtgcagcaa
     2461 ccaacaagaa aacactggca gattacgccc gtgccttatc cggagaggat gaatgacgcg
     2521 acaggaagaa cttgccgctg cccgtgcggc actgcatgac ctgatgacag gaaaacgggt
     2581 ggcaacggta cagaaagacg gacggcgagt ggagtttacg gccacttccg tgtctgacct
     2641 gaaaaaatac attgcggagc tggaagtgca gaccggcatg acacagcgac gcaggggacc
     2701 tgcaggattt tatgtatgaa aacgtcctcc attcccaccc ttctggggcc ggacggcatg
     2761 acatcgctgc gtgaatatgc cggttatcac ggcggtggca gcggatttgg tgggcagttg
     2821 cgggcgtgga acccaccgag tgaaagtgtg gatgcagccc tgttgcccaa ctttacccgt
     2881 ggcaatgccc gcgcggacga tctggtacgc aataacggct atgccgccaa cgccatccag
     2941 ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc
     3001 tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg
     3061 aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc
     3121 atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc
     3181 acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag
     3241 cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt
     3301 aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg
     3361 ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgtgcctc gttcattcac
     3421 gtttttgaac ccgtggagga cgggcagacc cgcggtgcaa atgtgtttta cagcgtgatg
     3481 gagcagatga agatgctcga cacgctgcag aacacgcagc tgcagagcgc cattgtgaag
     3541 gcgatgtatg ccgccaccat tgagagtgag ctggatacgc agtcagcgat ggattttatt
     3601 ctgggcgcga acagtcagga gcagcgggaa aggctgaccg gctggattgg tgaaattgcc
     3661 gcgtattacg ccgcagcacc ggtccgtctg ggaggcgcaa aagtgccgca cctgatgccg
     3721 ggggactcac tgaacctgca gacggctcag gacacggata acggctactc cgtgtttgaa
     3781 cagtcactgt tgcggtatat cgctgccggg ctgggtgtct cgtatgagca gctttcccgg
     3841 aattacgccc agatgagcta ctccacggca cgggccagtg cgaacgagtc gtgggcgtac
     3901 tttatggggc ggcgaaaatt cgtcgcatcc cgtcaggcga gccagatgtt tctgtgctgg
     3961 ctggaagagg ccatcgttcg ccgcgtggtg acgttacctt caaaagcgcg tttcagcttt
     4021 caggaagccc gcagcgcctg ggggaactgt gactggatag gctccggtcg tatggccatc
     4081 gatggtctga aagaagtaca ggaagcggtg atgctgatag aagccggact gagtacctac
     4141 gagaaagagt gcgcaaaacg cggtgacgac tatcaggaaa tttttgccca gcaggtccgt
     4201 gaaacgatgg agcgccgtgc agccggtctt aaaccgcccg cctgggcggc tgcggcattt
     4261 gaatccggac tgcgacaatc aacagaggag gagaagagtg acagcagagc tgcgtaatct
     4321 cccgcatatt gccagcatgg cttttaatga gccgctgatg cttgaacccg cctatgcgcg
     4381 ggttttcttt tgtgcgcttg caggccagct tgggatcagt cgcctgacgg atgcagtatc
     4441 cggcgacagc ctgactgccg gagaggcacc cgcggcgctg gcgttatccg gtgatgatga
     4501 cggaccacga caggcccgca gttatcaggt catgaacggc atcgccgtgc tgccggtgtc
     4561 cggtacgctg gtcagccgga cgcgggcgct gcagccgtat tcgggaatga ccggttacaa
     4621 cggcattatc gcccgtctgc aacaggctgc cagcgatccg atggtggacg gcattctgct
     4681 cgatatggac acaccgggcg ggatggtggc gggagcattt gactgtgctg acatcatcgc
     4741 ccgtgtgcgt gacataaagc cggtatgggc gctggccaac gacatgaact gcagtgcagg
     4801 tcagctgctt gccagcgccg cctcccggcg tctggtcacg cagaccgccc ggacaggctc
     4861 catcggcgtc atgatggctc acagtaatta cggcgctgcg ctggagaaac agggcgtgga
     4921 aatcacgctg atttacagcg gcagccataa ggtggatggc aacccctaca gccatctacc
     4981 gggtgatgtc cgggagacac tgcagtcccg gatggatgca acccgccgga tgtttgcgca
     5041 gaaggtgtcg gcatataccg gcctgtccgt gcaggctgtg ctggataccg aggctgcagt
     5101 gtacagcggt caggaggcca ttgatgccgg actggctgat gaacttgtca acagcaccga
     5161 tgcgatcacc gttatgcgtg atgcactgga tgcacgtaaa tcccgtctct caggagggcg
     5221 aatgaccaaa gagactcaat caacaactgt ttcagccact gcttcgcagg ctgacgttac
     5281 tgacgtggtg caagcgacgg agggcgaaaa cgccagcgcg gcgcagccgg acgtgaacgc
     5341 gcagatcacc gctgcggttg cggcagaaaa cagccgcatt atggggatcc tcaactgtga
     5401 ggaagctcac ggacgcgaag aacaggcacg tgtgctggcc gaaacccccg gtatgaccgt
     5461 ggaaacggcc cgccgcattc tggcagctgc accacagagt gcacaggcgc gcagtgacac
     5521 tgcgctggat cgtctgatgc agggggcacc ggcaccactg gctgcaggta acccggcatc
     5581 tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg atgtttatga cgagcaaaga
     5641 aacctttacc cattaccagc cgctgggcaa cagtgacccg gcacatacgg caaccgcgcc
     5701 cggcggattg agtgcgaaag cgcctgcaat gaccccgctg atgctggaca cctccacccg
     5761 taagctggtt gcgtgggatg gcaccaccga cggtactgcc gttggcattc tggcggttga
     5821 tgctgaccag accagcacca cgctgacgtt ctacaagtcc ggcacgttcc gttatgagga
     5881 tgtgctctgg ccggaggctg ccagcgacga gacgaaaaaa cggaccgcgt ttgccggaac
     5941 ggcaatcagc atcgtttaac cttacccttc atcactaaag gccgcctgtg cggctttttt
     6001 tacgggattt ttttatgtcg atgtacacaa ccgcccagct gctggcggca aatgagcaga
     6061 aatttaagtt tgatccgctg tttctgcgtc tctttttccg tgagagctat cccttcacca
     6121 cggagaaagt ctatctctca caaattccgg gactggtaaa catggcgctg tacgtttcgc
     6181 cgattgtttc cggtgaggtt atccgttccc gtggcggctc cacctctgaa tttacgccgg
     6241 gatatgtcaa acccaagcat gaggtgaatc cgcagatgac cctgcgtcgc ctgccggatg
     6301 aagatccgca gaatctggcg gacccggctt accgccgccg tcgcatcatc atgcagaaca
     6361 tgcgagacga agagctggcc attgctcagg tcgaagagat gcaggcagtt tctgctgtgc
     6421 ttaagggcaa atacaccatg accggtgaag ccttcgatcc ggttgaggtg gatatgggcc
     6481 gcagtgcggc gaacaacatc acacagtccg gtggtacgga gtggagcaag cgtgacaagt
     6541 ccacgtatga cccgaccgac gatatcgaag cctatgcgct gaacgccagc ggcgtggtga
     6601 atatcatcgt gtttgatccg aaaggctggg cgctgttccg ttccttcaaa gccgtcaagg
     6661 agaagctgga tacccgtcgc ggctctcatt ccgagctgga gacagcggta aaagacctgg
     6721 gcgaagcggt gtcctataag gggatgtatg gcgatacggc gatcgtcgtg tattccggac
     6781 agtacgtgga aaacgacgtc aaaaagaact tcctgccgga caacacgatg gtgctgggga
     6841 acactcaggc acgcggtctg cgcacctatg gctgcattca ggatgcggac gcacagcgcg
     6901 aaggtattaa cgcctctgcc cgctacccga aaaactgggt gaccaccggc gatccggcgc
     6961 gtgagttcac catgattcag tcagcaccgc tgatgctgct ggctgatcct gatgcgttcg
     7021 tgtccgtaca actggcgtaa tcatggccct tcggggccat tttctctctg tggaggagtc
     7081 catgacgaaa gatgaactga ttgcccgtct tcaggtgctg ggtgagcaac tgaaccgtga
     7141 tgtcagcctg acggggacga aagaagaact ggtgctccgt gtggcagagc tggaagagga
     7201 gcttgatgac acggatgacg ctgccggtca ggacacatct gtcagcccgg aaaatgcgct
     7261 gaccggacat gaaaatgagg tggtatcagc gcagccggat accgtgattg atacggctgc
     7321 tctggtcacg gtcgtggcac tggtgacgct gcatactgat gcacttcacg ccacgcggga
     7381 tgagcctgtg gcatttgtgc tgccgggaac ggcgtttcgt gtctctgccg gtgtggcagc
     7441 cgaaatgaca gaacatggcc tggccagaat gcaataacgg gaggcgctgt ggctgattcc
     7501 gataacctgt tcgatgctgc cattgcccgc gccgatgaaa cgatacgcgg gtacatggga
     7561 acgtcagcca ccatgacatc cggtgagctg tccggtgctg tgatacgtgg tgtttttgat
     7621 gaccctgaaa atatcagcta tgccggacag ggggtgcgcg ttgaaggctc cagcccgtcc
     7681 ctgtttgtcc ggactgatga tgtgcggcag ctgcggcgtg gagacacact gaccatcggc
     7741 gaggaaaact tctgggtgga ccggatttcg ccggatgatg gcggaagctg tcatctctgg
     7801 cttgggcgtg gcgtgccgcc tgccgttaac cgtcgccgct gaaaggggga tgtatggcca
     7861 taaaaggtct tgagcaggcc gttgaaaacc tcagccgtat cagcaaaacg gcggtgcctg
     7921 gtgccgccgc aatggccatt aaccgcgttg cgtcatccgc gatatcgcag tctgcgtcac
     7981 aggttgcccg tgagacaaag gtacgccgga aactggtaaa ggaaagggcc aggctgaaaa
     8041 gggccacggt caaaaatccg caggccagaa tcagggttaa ccggggggat ttgcccgtaa
     8101 tcaagctggg taacgcgcgg gttgtccttt cccgccgcag acgtcgtaaa aaggggcagc
     8161 gttcatccct gaaaggtggc ggcagcgtgc ttgtggtggg aaaccgtcgt atttccggcg
     8221 cgtttattca gcaactgaaa aatggccggt ggcatgtcat gcagcgtgtg gccgggaaaa
     8281 accgttaccc cattgatgtg gtgaaaatcc cgatggcggt gccgctgacc acggcgttta
     8341 aacagaatat tgaacggata cggcgtgaac gtcttccgaa agagctgggc tatgcgctgc
     8401 agcatcaact gagaatggta ataaagcgat gaaacatact gaactccgtg cagccgtact
     8461 ggatgcactg gagaagcatg acaccggggc gacgtttttt gatggtcgcc ccgctgtttt
     8521 tgatgaggcg gattttccgg cagttgccgt ttatctcacc ggcgctgaat acacgggcga
     8581 agagctggac agcgatacat ggcaggcgga gctgcatatt gaagttttcc tgcctgctca
     8641 ggtgccggat tcagagctgg atgcgtggat ggagtcccgg atttatccgg tgatgagtga
     8701 tatcccggca ctgtcagatt tgatcaccag tatggtggcc agcggctatg actaccggcg
     8761 cgacgatgat gcgggcctgt ggagttcagc cgatctgact tatgtcatta cctatgaaat
     8821 gtgaggacgc tatgcctgta ccaaatcctg taatgccggt gaaaggtgcc gggaccaccc
     8881 tgtgggttta taagggaagc ggtgaccctt atgcgaatcc gctttcagac gttgactggt
     8941 cgcgtctggc aaaagttaaa gacctgacgc ccggcgaact gaccgctgag tcctatgacg
     9001 acagctatct cgatgatgaa gatgcagact ggactgcgac cgggcagggg cagaaatctg
     9061 ccggagatac cagcttcacg ctggcgtgga tgcccggaga gcaggggcag caggcgctgc
     9121 tggcgtggtt taatgaaggg gatacccgag cctataaaat ccgcttcccg aacggcacgg
     9181 tcgatgtgtt ccgcggctgg gtcagcagta tcggtaaggc ggtgacggcg aaggaagtga
     9241 tcacccgcac ggtgaaagtc accaacgtgg gccgtccgtc gatggcagaa gatcgcagca
     9301 cggtgacggc gacaaccggc atgactgtga cgcctgccag cacctcggtg gtgaaagggc
     9361 agagcaccac gctgaccgtg gcattccagc cggaaggcgc aaccgacaag agcttccgtg
     9421 cggtgtctgc ggataaaaca aaagccaccg tgtcggtcag tggtatgacc atcaccgtga
     9481 aaggtgttgc tgcaggcaag gtcaacattc cggtcgtatc cggtaatggt gaacttgctg
     9541 cggttgcaga aatcaccgtc accgccagtt aatccgggga gtcagcgatg ttcctgaaaa
     9601 ccgaatcatt tgaacataac ggtgtgaccg tcacgctttc tgaactgtca gccctgcagc
     9661 gaattgagca tctcgccctg atgaaacggc aggcagaaca ggcggagtca gacagcaacc
     9721 ggaagtttac tgtggaagac gccatcagaa ccggcgcgtt tctggtggcg atgtccctgt
     9781 ggcataacca tccgcagaag acgcagatgc cgtccatgaa tgaagccgtt aaacagattg
     9841 agcaggaagt gcttaccacc tggcccacgg aggcaatttc tcatgctgaa aacgtggtgt
     9901 accggctgtc tggtatgtat gagtttgttg tgaataatgc ccctgaacag acagaggacg
     9961 ccgggcccgc agagcctgtt tctgcgggaa agtgttcgac ggtgagctga gttttgccct
    10021 gaaactggcg cgagagatgg ggcgacccga ctggcgtgcc atgcttgccg ggatgtcatc
    10081 cacggagtat gccgactggc accgctttta cagtacccat tattttcatg atgttctgct
    10141 ggatatgcac ttttccgggc tgacgtacac cgtgctcagc ctgtttttca gcgatccgga
    10201 tatgcatccg ctggatttca gtctgctgaa ccggcgtgag gctgacgaag agcctgaaga
    10261 tgatgtgctg atgcagaaag cggcagggct tgccggaggc gtccgctttg gcccggacgg
    10321 gaatgaagtt atccccgctt ccccggatgt ggcggacatg acggaggatg acgtaatgct
    10381 gatgacagta tcagaaggga tcgcaggagg agtccggtat ggctgaaccg gtaggcgatc
    10441 tggtcgttga tttaagtctg gatgcggcca gatttgacga gcagatggcc agagtcaggc
    10501 gtcatttttc cggtacggaa actgatgcga aaaaaacagc ggcagtcgtt gaacagtcgc
    10561 tgagccgaca ggcgctggct gcacagaaag cggggatttc cgtcgggcag tataaagccg
    10621 ccatgcgtat gctgcctgca cagttcaccg acgtggccac gcagcttgca ggcgggcaaa
    10681 gcccgtggct gatcctgctg caacaggggg gtcaggttaa ggactccttc ggcgggatga
    10741 tccccatgtt cagggggctt gccggtgcga tcaccctgcc gatggtgggg gccacctcgc
    10801 tggcggtggc gaccggtgcg ctggcgtatg cctggtatca gggcaactca accctgtccg
    10861 atttcaacaa aacgctggtc ctttccggca atcaggcggg actgacggca gatcgtatgc
    10921 tggtcctgtc cagagccggg caggcggcag ggctgacgtt taaccagacc agcgagtcac
    10981 tgacggcgct ggtgaatgcc ggtgtgcgtg gtggtgagca gtttgaggcg atcagccaga
    11041 gtgtggcgcg tttctcctct gcatccggcg tggaggtgga caaggtcgct gaagccttcg
    11101 ggaagctgac cacagacccg acgtcgggac tgacagcgat ggcacgtcag ttccataacg
    11161 tgacggcgga gcagattgcg tatgttgctc agttgcagcg ttccggagat gaagccgggg
    11221 cattgcaggc ggcgaacgag gccgcaacga aagggtttga tgaccagacc cgccgcctga
    11281 aagagaacat gggcacgctg gagacctggg cagacaggac agcacgggca ttcaaatcca
    11341 tgtgggattc ggtgctggat attggtcgcc cggacactgc ccagggaatg ctggagaaag
    11401 cagaaaaggc ttttgatgag gcggacaaaa aatggcagtg gtatcagagc cggagccacc
    11461 ggcgcggtaa aacctcagca tttcttgcca atctccgggg agcatgggag gacagagcga
    11521 atgcgcaact tgggctttca gccgccacgt tgcaggccga tcttgaaaag gccagagaga
    11581 tggcagcaaa ggactgggcc gagtctgagg catcacggct gaaatatacc gaagaggcgc
    11641 agaaggctta cgaacggctg cagacgccgc tggagaaata taccgcccgt caggaagaac
    11701 tgaacaaggc actgaaagac gggaaaatcc tgcaggcgga ttacaacacg ctgatggcgg
    11761 cggcgaaaaa ggattatgaa gcgacgctga aaaagccgaa acagtccggc gtgaaggtgt
    11821 ctgcgggcga tcgtcaggaa gacagtgctc atgctgccct gctgacgctt caggctgaac
    11881 tccggacgct ggagaagcat gccggagcaa atgagaaaat cagccagcag cgccgggatt
    11941 tgtggaaggc ggagagccag ttcgcggtac tggaggaggc ggcgcaacgt cgccagctgt
    12001 ctgcacagga gaaatccctg ctggcgcata aagatgagac gctggagtac aaacgccagc
    12061 tggctgcact tggcgacaag gttacgtatc aggagcgcct gaacgcgctg gcgcagcagg
    12121 cggataaatt cgcacagcag caacgggcaa aacgggccgc cattgatgcg aaaagccggg
    12181 ggctgactga ccggcaggca gaacgggaag ccacggaaca gcgcctgaag gaacagtatg
    12241 gcgataatcc gctggcgctg aataacgtca tgtcagagca gaaaaagacc tgggcggctg
    12301 aagaccagct tcgcgggagc tggatggcag gcctgaagtc cggctggagt gagtgggaag
    12361 agagcgccac ggacagtatg tcgcaggtaa aaagtgcagc cacgcagacc tttgatggta
    12421 ttgcacagaa tatggcggcg atgctgaccg gcagtgagca gaactggcgc agcttcaccc
    12481 gttccgtgct gtccatgatg acagaaattc tgcttaagca ggcaatggtg gggattgtcg
    12541 ggagtatcgg cagcgccatt ggcggggctg ttggtggcgg cgcatccgcg tcaggcggta
    12601 cagccattca ggccgctgcg gcgaaattcc attttgcaac cggaggattt acgggaaccg
    12661 gcggcaaata tgagccagcg gggattgttc accgtggtga atttgtcttc acgaaggagg
    12721 caaccagccg gattggcgtg gggaatcttt accggctgat gcgcggctat gccaccggcg
    12781 gttatgtcgg tacaccgggc agcatggcag acagccggtc gcaggcgtcc gggacgtttg
    12841 agcagaataa ccatgtggtg attaacaacg acggcacgaa cggtcagata ggtccggctg
    12901 ctctgaaggc ggtgtatgac atggcccgca agggtgcccg tgatgaaatt cagacacaga
    12961 tgcgtgatgg tggcctgttc tccggaggtg gacgatgaag accttccgct ggaaagtgaa
    13021 acccggtatg gatgtggctt cggtcccttc tgtaagaaag gtgcgctttg gtgatggcta
    13081 ttctcagcga gcgcctgccg ggctgaatgc caacctgaaa acgtacagcg tgacgctttc
    13141 tgtcccccgt gaggaggcca cggtactgga gtcgtttctg gaagagcacg ggggctggaa
    13201 atcctttctg tggacgccgc cttatgagtg gcggcagata aaggtgacct gcgcaaaatg
    13261 gtcgtcgcgg gtcagtatgt tgcgtgttga gttcagcgca gagtttgaac aggtggtgaa
    13321 ctgatgcagg atatccggca ggaaacactg aatgaatgca cccgtgcgga gcagtcggcc
    13381 agcgtggtgc tctgggaaat cgacctgaca gaggtcggtg gagaacgtta ttttttctgt
    13441 aatgagcaga acgaaaaagg tgagccggtc acctggcagg ggcgacagta tcagccgtat
    13501 cccattcagg ggagcggttt tgaactgaat ggcaaaggca ccagtacgcg ccccacgctg
    13561 acggtttcta acctgtacgg tatggtcacc gggatggcgg aagatatgca gagtctggtc
    13621 ggcggaacgg tggtccggcg taaggtttac gcccgttttc tggatgcggt gaacttcgtc
    13681 aacggaaaca gtgacgccga tccggagcag gaggtgatca gccgctggcg catcgagcag
    13741 tgcagcgaac tgagcgcggt cagtgcctct tttgtactgt ccacgccgac ggaaacggac
    13801 ggtgccgttt ttccgggacg tatcatgctg gccaacacct gcacctggac ctatcgcggt
    13861 gatgagtgcg gttatcacgg tccggcggtc gcggatgaat atgatcagcc gacgtccgat
    13921 atcacgaagg ataaatgcag caaatgcctg agcggctgta agtttcgcaa taacgtcggc
    13981 aactttggcg gcttcctttc cattaacaaa ctttcgcagt gaatcccatg acagagacag
    14041 aatcagcgat tctggcgcac gcccggcgat gtgcgccagc ggagtcgtgc ggcttcgtgg
    14101 tgagaacacc ggagggggaa agatattttc cctgcgtgaa tatctccggt gagccggagg
    14161 cgtatttccg gatgtcgccg gaggactggc tgcgggcaga gatgcagggt gagattgtgg
    14221 cgctggtcca cagccacccc ggtggtctgc cctggctgag tgagaccgac cggcggctgc
    14281 aggtgcagag tgatttgccg tggtggctgg tttgccgggg ggcgattcac aagttccgct
    14341 gtgtgccaca tctttccggg cggcgctttg agcacggggt gacggactgt tacacgctgt
    14401 tccgggatgc ttaccatctg gcggggattg agatgccgga ttttcatcgc gaggatgact
    14461 ggtggcgtca cggtcagaat ctctatctgg ataatctgga ggccacaggg ctgtatcagg
    14521 tgccgttgtc atcagcacaa ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc
    14581 cgaatcatgc cgccatttac tgtggtgaca gcgagctgct gcaccatatt cctgaacaac
    14641 tgagcaaacg agagaggtac accgacaaat ggcagcgacg cacacactcc ctctggcgtc
    14701 accgggaatg gcacgcatct gcctttacgg ggatttgcaa cgatttggcc gccgcatcga
    14761 ccttcgtgtg aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggcgtt
    14821 tcgtcagaaa ctgagcgacg gctggtatca ggtacggatt gccgggcggg acgtcagcac
    14881 gtccgggtta acggcgcagt tacatgagac tctgcctgac ggcgctgtga ttcatattgt
    14941 tcccagagtc gccggggcca agtcaggggg cgtattccag attgttctgg gggctgccgc
    15001 cattgccgga tcattcttta ctgccggagc cacccttgca gcatgggggg cagccattgg
    15061 ggccggtggt atgaccggca tcctgttttc tctcggtgcc agtatggtgc tcggtggtgt
    15121 ggcgcagatg ctggcaccga aagccagaac tccccgtaca cagacaacgg ataacggtaa
    15181 gcagaacacc tatttctcct cactggataa catggttgcc cagggcaatg ttctgcctgt
    15241 tctgtacggg gaaatgcgcg tggggtctcg cgtggtttct caggagatca gcacggcaga
    15301 cgaaggggat ggtggtcagg ttgtggtgat tggtcgctga tgaaaaacgt ttatgtgaaa
    15361 ccgcctccgg gcggttttgt cgtttatgga gcgtgaggaa tgggtaaagg cagcagtaag
    15421 gggcatactc cgcgcgaagc gaaggacaac ctgaagtcca cgcagttgct gagtgtgatc
    15481 gatgccatca gcgaagggcc gattgaaggt ccggtggatg gattaaaaag cgtgctgctg
    15541 aacagtacgc cagtgctgga cagtgagggg aatactaaca tctccggcgt cacggtggtg
    15601 ttccgggccg gtgagcagga gcagacaccg ccggagggat ttgaatcctc cggctccgag
    15661 acggtgctgg gtacggaagt gaaatacgac acgccgatca cctggaccat cacgtcggca
    15721 aacattgacc gactgcgtgt taccttcggc gtgcaggcac tggtggaaac cacctcaaag
    15781 ggggacagga atccgtcgga agtccgcctg ctggttcaga tacagcgtaa tggtggctgg
    15841 gtgacggaaa aagacatcac cattaagggc aaaaccacct cgcagtatct ggcctcggtg
    15901 gtggtgggta acctgccgcc gcgcccgttc agtatacgga tgcgcaggat gacgccggac
    15961 agcaccacag accagctgca gaacaaaacg ctctggtcgt catacaccga aatcatcgat
    16021 gtgaaacagt gctacccgaa cacggcactg gtcggtgtgc aggtggactc ggagcagttc
    16081 ggcagccagc aggtgagccg taattatcat ctgcgcgggc gtattctgca ggtgccgtcg
    16141 aactataacc cgcagacgcg gcaatacagc ggtatctggg acggaacgtt taaaccggca
    16201 tacagcaaca acatggcctg gtgtctgtgg gatatgctga cccatccgcg ctacggcatg
    16261 gggaaacgtc ttggtgcggc ggatgtggat aaatgggcgc tgtatgtcat cggccagaat
    16321 tgcgaccagt cggtgccgga cggctttggc ggcacggagc cgcgcatcac ctgtaatgcc
    16381 tggctgacca cacagcgcaa ggcgtgggat gtgctcagtg atttctgctc ggcgatgcgc
    16441 tgtatgccga tatggaacgg gcagacgctg acgttcgtgc aggaccgacc atcagataag
    16501 gtgtggacct ataaccgcag taatgtggtg atgccggatg atggcgcgcc gttccgctac
    16561 agcttcagcg ccctgaagga ccgccataat gccgttgagg tgaactggat tgacccggat
    16621 aacggctggg agacggcgac agagcttgtg gaggacacgc aggccattgc ccgttacggt
    16681 cgtaacgtca cgaagatgga tgcctttggc tgtaccagtc gggggcaggc gcaccgcgcc
    16741 gggctgtggc tgattaaaac ggaactgctg gaaacgcaga ccgtggactt cagcgtgggt
    16801 gcggaagggc ttcgccatgt accgggggat gtcattgaaa tctgcgatga tgactatgcg
    16861 ggtatcagca ccggcgggcg cgtgctggcg gtgaacagcc agacccggac gctgacgctc
    16921 gaccgtgaaa tcacgctgcc atcctccggt accacgctga taagcctggt tgacggaagt
    16981 ggcaatccgg tcagcgtgga ggtccagtcc gtcaccgacg gcgtgaaggt aaaagtgagc
    17041 cgtgttcctg acggcgttgc cggatacagc gtgtgggggc tgaagctgcc gacgctgcgc
    17101 cagcgactgt tccgctgcgt gagtatccgt gagaacgacg acggcacgta tgccatcacc
    17161 gccgtgcagc atgtgccgga aaaagaggcc atcgtggata acggggcgca ctttgacggc
    17221 gaccagagcg gcacggtgaa tggtgtcacg ccgccagcgg tgcagcacct gactgccgaa
    17281 gtcaccgcag acagcgggga atatcaggtg ctggcgcgct gggacacacc gaaggtggtg
    17341 aagggcgtga gcttcctgct ccgtctgacc gtaacagcgg acgacggcag tgagcggctg
    17401 gtcagcacgg cccggacgac ggaaaccaca taccgcttca cacaactggc gttggggaac
    17461 tacaggctga cagtccgggc ggtaaatgcg tggggacagc agggcgatcc ggcatcggta
    17521 tcgttccgga ttgccgcacc ggcagcgccg tctcggattg agctgacacc gggctatttt
    17581 cagataaccg ccacgccgca tcttgcggtt tatgacccga cggtacagtt tgagttctgg
    17641 ttctcggaaa aacggattgc ggatatcagg caggttgaaa ccagcgcgcg ttatcttggt
    17701 acggcactgt actggatagc cgccagtatc aatatcaaac cgggccatga ttattatttt
    17761 tacgttcgca gtgtgaacac cgttggcaaa tcggcattcg tggaggctgt cggtcagccg
    17821 agtgatgatg catcaggcta tctggatttt ttcaaaggcg agatagggaa aacccatctg
    17881 gctcaggagc tgtggacgca gattgataac ggtcagcttg cgcctgacct gactgaaatc
    17941 aggacgtcca taacggatgt cagcaatgaa ataacacaga ccgtcaataa gaaactggaa
    18001 gaccagagtg cagcgatcca gcagatacag aaggttcagg ttgatacaaa taataacctg
    18061 aacagcatgt gggcagtgaa gctgcagcag atgcaggacg gacgccttta tattgcgggt
    18121 atcggtgccg gtattgagaa cacccctgac ggcatgcaga gtcaggtgct gctggcagca
    18181 gacaggattg cgatgattaa tcctgcgaat ggcaacacaa agccgatgtt tgttggtcag
    18241 ggcgatcaga tattcatgaa tgaagtgttc ctgaaacgcc tgacggcccc caccattacc
    18301 agcggcggta atcctccggc attttccctg acatcagacg ggagactgac ggcgaaaaat
    18361 gcggatatca gtggcagtgt gaatgcgaac tcaggaacgc tcaacaatgt cacgattaac
    18421 cagaactgta cgattaaggg catgctggag gcgacccagg tcagaggaga tttcgttaaa
    18481 gctgtatcaa aagccttccc gaaaaaagtc ggtacgtggg gtaacacgga aacaccaaac
    18541 ggtacggtta cagtcaccat cagcgatgat cataactttg accgccagat tattattccg
    18601 cccattattt ttaacggtat agcgtatgac gatccgggga gcggaaataa cccaggaggc
    18661 acgcgataca cgggttatgg ttttgaagtt cgcaaaaacg gcgtattaat cgcatccaga
    18721 gaaactaaag gggccattcc cggtagttac agtgcagtta ttgatatgcc gagtggcagg
    18781 ggaagcgtca ctctggagtt taagattttc cagaaaggca atcagggggc aggcaatatc
    18841 accgactgta cggtgattgt gaccaaaaaa gccgcttccg gcatcagtat tcgttgaaat
    18901 atttataacc ccaataaagg gcgtcaggaa tgacgccttt tttattgcag aaaagcgaga
    18961 ggtaattatg cgtaaagttt gtgcagcaat tttgtccgca gccatttgtc tggccgtatc
    19021 cggtgcgcct gcatgggcgt ctgaacatca gtccacgctg agcgcggggt atcttcatgc
    19081 ctcgacgaac gtccccggca gcgatgatct gaacgggatt aacgtgaaat accgttatga
    19141 gtttacggac acactggggc tggtgacgtc attcagctat gcaggagaca agaatcgcca
    19201 gctgacccgt tacagcgata cccgctggca tgaagattcc gttcgtaacc gctggttcag
    19261 cgtaatggcg gggccgtctg tgcgcgtgaa tgaatggttc agcgcgtatg cgatggcggg
    19321 tgtagcttac agccgtgtgt cgactttctc cggggattat cttcgcgtaa ctgacaacaa
    19381 ggggaaaaag cacgatgtgc tgaccggaag tgatgacggt cgccacagca acacgtctct
    19441 ggcgtggggg gctggcgtgc agtttaaccc gaccgaatcc gtggccattg atattgctta
    19501 tgaaggctcc ggcagtggtg actggcgcac tgacggtttc atcgtgggtg tcggttataa
    19561 attctgatta gccaggtaac acagtgttat gacagcccgc cggttcaggc gggctttttt
    19621 gtggggtgaa tatggcagta aagatttcag gtgtactgaa agacggcaca ggaaaaccgg
    19681 tacagaactg cacaatccag ctgaaagcaa aacgtaacag caccacggtg gtggtgaaca
    19741 cgctggcctc agaaaatccg gatgaagccg ggcgttacag catggacgtt gagtacgggc
    19801 agtacagcgt tattctgttg gtggagggat tcccgccgtc acatgccggg accatcaccg
    19861 tgtatgaaga ttcccgaccc ggtacgctga atgattttct cggtgccatg acggaggatg
    19921 atgcccgtcc tgaggcactg cgccgttttg aactgatggt ggaagaggtg gcgcgtaacg
    19981 cgtccgcggt ggcacagaac acggcagccg cgaagaagtc agccagcgat gccagcacat
    20041 cagcccgtga ggcggcaacc catgcgactg atgctgcaga ttcagcacgt gcagccagca
    20101 cgtcagccgg acaggccgct tcgtcggctc agtcagcgtc ttccagcgca ggaacggcat
    20161 caacaaaggc cactgaagcc tcaaaaagtg ctgccgctgc agagtcctca aaaagcgtgg
    20221 cagctaccag tgccggtgcg gcgaaaacgt cagaaacgaa tgccgcagcg tcagaacaat
    20281 cagccgccac ttctgcatcc accgcgacca cgaaagcgtc agaagctgcc acctcagccc
    20341 gggatgcggc ggcctcaaaa gaggcagcga aatcatcaga aacgaacgca tccttgagcg
    20401 ccagtagcgc agcttcctcg gcaacggcgg caggaaattc cgcgaaggcg gcaaaaacgt
    20461 ccgagacgaa cgccaggtct tctgagacgg cagcgggaca gagcgcctca gctgcggcag
    20521 gctcaaaaac agcggctgcg tcgtctgcca gtgccgcgtc aacaagtgcc gggcaggcct
    20581 cagccagtgc caccgccgcc ggaaaatcgg cagaaagcgc cgcatcatcc gcttcaacag
    20641 ccacaacgaa ggctggcgaa gccactgagc aggccacggc agcagcgagg tcagcttccg
    20701 cagcgaagac atccgaaacg aacgcgaaag cgtcggaaac ccgtgcagaa tcctcaaaaa
    20761 cggcagcggc atcgtccgcc agttcggcgg cgtcatcggc atcatcggcg tctgcttcaa
    20821 aagatgaggc gaccagacag gcgtcagcag cgaagggcag cgccacgacg gcatccacga
    20881 aggcgacaga ggctgctggt agtgcgaagg cagcagctca gagcaaaagt acggcggaat
    20941 ctgcagcaac gcgcgctgag acagcggcaa aacgggcaga ggatattgca tccgccgtgg
    21001 cgcttgagga tgcgagcacg acgaaaaagg ggatagtaca gctcagtagt gcgactaaca
    21061 gcacttccga gtcactggcg gcaacaccaa aagccgttaa ggccgcgtat gacctggcta
    21121 gcgggaaata caccgcacag gatgcaacga cagcacagaa agggataatc cagctaagca
    21181 gcgcgaccaa cagcacgtct gaaacgctgg cggcaacgcc aaaggcagta aaagcagcca
    21241 atgacaatgc tgagaaacgt ctgcagaaag atcagaacgg tgcggatatc cctggcaaag
    21301 acacctttac gaaaaatatt ggtgcctgcc gtgccttcgg tgggtcagta agcacaacaa
    21361 caggaaactg gacgactgca cagtttatcg agtggctgga ttctcaggga gcatttaacc
    21421 atccatactg gatgtgcaag ggttcctggt cttatggcaa taataaaatc attactgata
    21481 ctggctgcgg taatattcat ctcgccggag ctgtcattga agtaatgggg ataaagtcag
    21541 cgatgacgat ccgcattacc actccgacca catccagcgg tggcggaaca accaacgccc
    21601 agtttaccta tattaatcac ggaacagatt attcacctgg ctggcgaagg gactataact
    21661 ccagaaataa gccaacggca tcagagatcg gggcgctacc gtcaggtgga acagcagtat
    21721 catcagttaa tctggcttca aaaggtcggg tagccgcgct gacagacaat acgcaggggg
    21781 cagcaggtct tgagttatac gaggtgtata acaacggata tccaacaacg tatggaaata
    21841 tcattcacct gaaagggatg acagccgttg gcgaaggtga gttactcatc ggctggagtg
    21901 gtacaagcgg tgctcatgct ccggcattta ttcgttcacg acgggatacg accgacgcaa
    21961 actggtcgcc gtgggcgcag ctttacacct cggctcatcc tcctgcagag ttttatccag
    22021 tcggtgcacc aatcccgtgg ccatcagata ccgttccgtc tggttatgtc ctgatgcagg
    22081 ggcagacttt tgacaaatct gcatacccga aacttgcagc cgcttatccg tcaggcgtga
    22141 tccctgatat gcgtggctgg acgattaagg gcaaacctgc cagtggtcgg gccgtattgt
    22201 ctcaggaaca ggacggcatt aaatcgcaca cccacagcgc cagcgcatcc agtacggatt
    22261 tggggacgaa aaccacatcg tcgtttgatt acggcactaa atccacgaat aacatcgggg
    22321 cgcatacgca cagtgtgagc ggtacagccg caagtgccgg aaaccatact catagtgtca
    22381 caggcgcatc agcagtcagc cagtggtcac aaaatgggtc agtacataag gtagtgtctg
    22441 cggccagtgt gaatacaagt gctgcaggag cgcacactca tagtgtcagc ggcacagctg
    22501 catctgcagg tgctcacgca catactgtcg gtattggtgc tcatacgcac tcggttgcga
    22561 ttggctcaca tggacacacc atcaccgtta acgctgctgg taacgcggaa aacaccgtca
    22621 aaaacatcgc atttaactat attgtgaggc ttgcatgatt acgctcattc tttctgcacc
    22681 agtaaccgaa atggctgaag catttaagcg ggtatttgca aatgcagata atgtgaatat
    22741 tgtcggaaag ccatttgaaa caatcagaga atttgactgc atggtaagtg cggcaaatag
    22801 tttcggcctg atggatggcg gtgttgatgc cgccattacc acattcttcg gttctcagct
    22861 acaggcccgc gttcagaatc atattcttcg tgaatatctc ggcgagcagc ctgtaggttc
    22921 tgcatttgtt attgaaacgg ggcataatta tcacccctgg ctggtacatg cgccaacaat
    22981 gcgtgttccg ttgacaattg acggaacaga cgctgtatat aacgcaactt gggcagctct
    23041 acttgctatc tttcagcaca ataaaaacgc aacgacagac aggaaaataa agacggtggt
    23101 attccctgca atgggagccg gatgtggtca ggtgccgttt gaaagcgttg cccggcagat
    23161 gaagcaggca tgggataact ttaataaaaa aacagaatca attaactggg aatacgcaca
    23221 atcccgccag tcggcagtat ttggcacata tgcatactgt ccgggtaatt ctgtttgccg
    23281 ttatgcggat actaaatata ttggatgcgg cgattatcgg acgtattgct cacgttccgg
    23341 gcaggtctgt attaaccctg aacatcaggc tgatgatgta ctgatacaac atcaggctaa
    23401 taaccggttt cgccctggtt cgcatataca ccggattaat ccagaaaatc ctgtaggtaa
    23461 tgtcatctct ggcgcacata gccacggaag tagcatcgtt attggtgctc acacccatac
    23521 gctcaataaa caatattctg tctctgatat taagtagagg tgaacatgga tttcagaatg
    23581 agtgaacaac cacggaccat aacaatttat aatctgctgg ccggaactaa tgaatttatt
    23641 ggtgaaggtg acgcatacat tccgcctcat acaggtctgc cagcaaacag taccgatatt
    23701 gcaccgccag atattccggc tggtttcgtg gctgttttca acagtgatga gtcatcgtgg
    23761 catctcgttg aagatcatcg gggtaaaacg gtttatgacg tggcttccgg cgacgcgtta
    23821 tttatttctg aactcggctc attaccggaa aatgtcacct ggttatcccc ggaaggggag
    23881 tttcagaagt ggaacggcac agcctgggtg aaggatacgg aagcagaaaa actgttccgg
    23941 atccgggagg cggaagaaac aaaaaacagc ctgatgcagg tagccagtga gcatatagcg
    24001 ccgcttcagg atgctgtgga tctggagatc gcaacggaga aagaaacctt gttgctggaa
    24061 gcctggaaaa agtatcgggt gttgctgaac cgtgttgata catcaactgc accggatatt
    24121 gaatggccag ccctaccgta gggtaagata tataaattct ataattagaa gtatctttcc
    24181 atttaaggct aggaaggggg gcttggaaaa cgtaaggaat ctcacaccga gattattttt
    24241 atatatcagg tgtctgattt tttgctttag ctcttaaaat ggtttgccgc gaggttttga
    24301 attcttgggc aatgtcactt atatttatac ctgacttaat ttgctctagt accctctgta
    24361 tttgttcatc atttaacaca ggtgggcgac caaatcgctt tcctgcgccc cgtgctctta
    24421 atatccctga atgagtacgc tcaagtaaaa ggtctcgctc aaattcagcg actgctgaaa
    24481 ttacttgcat catcattttt ccaggtggac tggtcaggtc aacgcccccc aatgctaagc
    24541 aatgcacttt gatacctgct ttggtcagtt gttccaccgt tttactgata tccattggat
    24601 tacaaccgag gcgatccagt tttgtgacaa tcaatgcatc acctcttttc agtcgatcaa
    24661 gcaactggtt aaaaccagga cgctcactgg ttgctgctga gccgctaatt tgttcttcaa
    24721 ttatttgctg agatttgatg ttaaaacctg cactttcgat ttcccgacgt tgatttttgg
    24781 ttgtcttttc cagagttgat acccgacagt aagcataaat tcgagacata gtgagatctt
    24841 ctatacgaaa
//
LOCUS       DAZUCC010000057        24442 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZUCC010000057 DAZUCC010000000
VERSION     DAZUCC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.057"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            108..2288
                     /gene="katG"
                     /locus_tag="ACHY09_001993"
     CDS             108..2288
                     /gene="katG"
                     /locus_tag="ACHY09_001993"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFS3884971.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            2382..3287
                     /gene="yijE"
                     /locus_tag="ACHY09_001994"
     CDS             2382..3287
                     /gene="yijE"
                     /locus_tag="ACHY09_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFS3884972.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(3314..3931)
                     /locus_tag="ACHY09_001995"
     CDS             complement(3314..3931)
                     /locus_tag="ACHY09_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFS3884973.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(4207..5310)
                     /gene="gldA"
                     /locus_tag="ACHY09_001996"
     CDS             complement(4207..5310)
                     /gene="gldA"
                     /locus_tag="ACHY09_001996"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFS3884974.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(5321..5983)
                     /gene="fsa"
                     /locus_tag="ACHY09_001997"
     CDS             complement(5321..5983)
                     /gene="fsa"
                     /locus_tag="ACHY09_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS3884975.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(5995..8496)
                     /gene="ptsP"
                     /locus_tag="ACHY09_001998"
     CDS             complement(5995..8496)
                     /gene="ptsP"
                     /locus_tag="ACHY09_001998"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS3884976.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVMKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIPLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLAAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            8805..9884
                     /locus_tag="ACHY09_001999"
     CDS             8805..9884
                     /locus_tag="ACHY09_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFS3884977.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            9899..10219
                     /locus_tag="ACHY09_002000"
     CDS             9899..10219
                     /locus_tag="ACHY09_002000"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFS3884978.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            10270..12567
                     /locus_tag="ACHY09_002001"
     CDS             10270..12567
                     /locus_tag="ACHY09_002001"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS3884979.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGS
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRSGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            12533..13411
                     /locus_tag="ACHY09_002002"
     CDS             12533..13411
                     /locus_tag="ACHY09_002002"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFS3884980.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDVTQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSQENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            13413..13754
                     /locus_tag="ACHY09_002003"
     CDS             13413..13754
                     /locus_tag="ACHY09_002003"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFS3884981.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFDHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(13741..14592)
                     /locus_tag="ACHY09_002004"
     CDS             complement(13741..14592)
                     /locus_tag="ACHY09_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS3884982.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGATGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(14818..16551)
                     /locus_tag="ACHY09_002005"
     CDS             complement(14818..16551)
                     /locus_tag="ACHY09_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFS3884983.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(16734..19385)
                     /gene="ppc"
                     /locus_tag="ACHY09_002006"
     CDS             complement(16734..19385)
                     /gene="ppc"
                     /locus_tag="ACHY09_002006"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFS3884984.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(19687..20838)
                     /gene="argE"
                     /locus_tag="ACHY09_002007"
     CDS             complement(19687..20838)
                     /gene="argE"
                     /locus_tag="ACHY09_002007"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFS3884985.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            20992..21996
                     /gene="argC"
                     /locus_tag="ACHY09_002008"
     CDS             20992..21996
                     /gene="argC"
                     /locus_tag="ACHY09_002008"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFS3884986.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            22004..22780
                     /gene="argB"
                     /locus_tag="ACHY09_002009"
     CDS             22004..22780
                     /gene="argB"
                     /locus_tag="ACHY09_002009"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFS3884987.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            22841..24214
                     /gene="argH"
                     /locus_tag="ACHY09_002010"
     CDS             22841..24214
                     /gene="argH"
                     /locus_tag="ACHY09_002010"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFS3884988.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVESKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         5524 a         6245 c         6814 g         5859 t
ORIGIN      
        1 ccaacaatat gtaagatctc aactatcgca tccgtggatt aattcaatta taacttctct
       61 ctaacgctgt gtatcgtaac ggtaacactg tagaggggag cacattgatg agcacgtcag
      121 acgatatcca taacacgaca gccaccggca aatgcccgtt ccatcagggc ggtcacgacc
      181 agagcgcagg tgggggcaca accacgcgcg actggtggcc aaatcaactc cgtgttgacc
      241 tgttaaacca acattctaat cgttctaacc cgctgggtga ggactttgac taccgcaaag
      301 aattcagcaa attagattac tacggactga aaaaagatct gaaagccctg ctgacagaat
      361 ctcaaccgtg gtggcctgcc gactggggca gctacgccgg tctgtttatt cgtatggcct
      421 ggcacggtgc gggtacttac cgttcaatcg atgggcgcgg cggcgcgggt cgtggtcagc
      481 aacgttttgc accgctgaac tcctggccgg ataacgtaag cctcgataaa gcgcgccgcc
      541 tgttgtggcc aatcaaacag aaatatggtc agaaaatctc ctgggccgac ctgtttatcc
      601 tcgcgggtaa cgtggcgctg gaaaactccg gcttccgtac cttcggtttt ggtgccggtc
      661 gtgaagacgt ctgggaaccg gatctggatg ttaactgggg tgatgaaaaa gcctggctga
      721 cccaccgtca tccggaagca ctggcgaaag caccgctggg cgcaaccgag atgggcctga
      781 tttacgttaa cccggaaggc ccggatcaca gcggcgaacc gctttctgcg gcagcagcta
      841 tccgcgcgac cttcggcaac atgggcatga acgacgaaga aaccgtggcg ctgattgcgg
      901 gtggtcatac gctgggtaaa actcacggtg ccggtccgac atcaaatgta ggtcctgatc
      961 cagaagctgc accgattgaa gaacaaggtt taggctgggc gagcacttac ggcagcggcg
     1021 ttggcgcaga tgccattacc tctggtctgg aagtagtctg gacccagacg ccgacccaat
     1081 ggagcaacta tttcttcgag aacctgttca aatatgagtg ggtacaaact cgcagcccgg
     1141 ctggcgcaat ccagttcgaa gcggtagacg caccggaaat tatcccggat ccgtttgatc
     1201 cgtcgaagaa acgtaaaccg acgatgctgg tgaccgacct gacgctgcgt tttgatcctg
     1261 agttcgagaa gatctctcgt cgtttcctca acgatccgca ggcgttcaac gaagcctttg
     1321 ctcgtgcttg gttcaaactg acgcacaggg atatggggcc gaaatctcgt tacatcgggc
     1381 cggaagtacc gaaagaagat ctgatctggc aagatccgct gccgcagccg atctacaacc
     1441 cgaccgagca ggacattatc gatctgaaat tcgcgattgc ggattctggt ctgtctgtta
     1501 gtgagctggt atcggtggcc tgggcgtctg cttctacctt ccgtggtggc gacaaacgtg
     1561 gtggtgccaa cggtgcgcgt ctggcattaa tgccgcagcg tgactgggat gtgaacgccg
     1621 cagccgttcg tgctctgcca gttctggaga aaattcagaa agagtctggc aaagcttcgc
     1681 tggcggatat catcgtgctg gcaggtgtgg ttggcgttga gaaagccgca agcgccgcag
     1741 gtttgagcat tcatgtaccg tttgcgccgg gccgcgttga tgcgcgtcag gatcagactg
     1801 acattgagat gttcgaactg ctggagccaa ttgctgacgg tttccgtaac tatcgcgctc
     1861 gtctggacgt ttccaccacc gagtcactgt tgattgataa agcacagcaa ctgacgctga
     1921 ccgcgccgga aatgactgcg ctggtgggcg ggatgcgcgt actgggtgcc aacttcgatg
     1981 gcagcaaaaa cggcgtcttc actgaccgcg ttggcgtatt gagcaatgac ttcttcgtga
     2041 acttgctgga tatgcgttac gagtggaaag cgaccgacga atcgaaagag ctgttcgaag
     2101 gccgtgaccg cgaaactggc gaagtgaaat acacagccag ccgtgcggat ctggtctttg
     2161 gttctaactc tgtcctgcgt gcggtggcgg aagtctacgc cagcagcgat gcccacgaga
     2221 agtttgttaa agacttcgtg gcagcgtggg tgaaagtgat gaacctcgac cgtttcgacc
     2281 tgctgtaatc tgaccttctt cagcgactgc ctttcatgca gtcgctgaag tttctttacc
     2341 ggcgtatagt gtccacagga aaactacaca ctggatctct catgtctgcc gcaggaaaga
     2401 gcaacccact ggcaatcagt ggcctggttg tgctcacact tatctggagt tatagctgga
     2461 ttttcatgaa gcaagtcacc agctacatcg gtgccttcga ctttaccgcc ttacgctgca
     2521 ttttcggcgc tctcgtttta ttcatcgtcc ttttattacg tggtcgcgga atgcgcccga
     2581 caccgtttaa atacacctta gccattgccc tgttacaaac ctgcgggatg gtcggcctgg
     2641 cgcagtgggc gctggtcagc ggaggagcgg ggaaggtggc aatcctgagc tataccatgc
     2701 cgttctgggt ggtaattttc gccgcgttgt ttctcggtga acgcctgcga cgcggacaat
     2761 atttcgcgat tctgattgcc gctttcggct tatttttggt gttgcagccg tggcaactcg
     2821 atttctcttc gatgaaaagc gccatgctgg caatcctctc cggtgtgagt tggggggcga
     2881 gcgcgattgt tgctaaacgt ctgtatgccc gccatccgcg cgtggattta ttgtcgctaa
     2941 catcctggca gatgctgtat gcggcgctgg tgatgagcgt ggtcgcttta ctggtgccgc
     3001 aacgtgaaat tgactggcaa cccaccgtgt tctgggcgct ggcctacagt gcgattctgg
     3061 cgacagcgct ggcgtggagc ttatggttgt ttgtattgaa aaacttgcct gccagtattg
     3121 ccagcttaag cacactggcc gttcccgttt gcggcgtact cttttcctgg tggctgctcg
     3181 gtgagaatcc gggggcagtt gaaggtagcg gtattgtgct gattgtactg gcactggcgc
     3241 tggtgagccg taagaaaaaa gaagccgtca gtgtaaaaag gatctgaatt ttttcttcat
     3301 gtggggagat ctcttattta gcaaaatacc gatagtgccc caccatccgc cagctaaaca
     3361 gtacatcttc ttcctgcgcg cctgcgccaa tgttatgtat caccagcggc gtaccgtcgc
     3421 gggcgaagcc atctgacacc accccaatat gtgccagccc gttatctaat cgccaggaga
     3481 caatatcgcc cgcttgatag tcactggggt tcttgctggt ggggcgtgtt ttatcgtggc
     3541 gggtaaacca ggtttccaga ttaggcaccc ggcggtgatc gatgttgctg tccgggcgct
     3601 ttaactgcca tttttgcggg tacgcagcaa aattcttcgc catatcttcg tgcaccagtt
     3661 tctgcaaatc gaccttctgg ctgcgcaatg cacggatcac cacatcggaa catacgccgc
     3721 gttcttgcgg gacatcaccg ccaggatagg taagctgcac atacgccgga tcgtaaaata
     3781 gcgtgctgcc gatttgctgt ctggcgccgt ctgcgatggc aagattggtg ttggcctgga
     3841 tttgtaccac ggttggtgga acggcgggag attttaagga gtaactggta aataccgtca
     3901 gaagactgaa taacgccagt gaagctttca tctgtttcgt ccgtgaaaat aagagtcaca
     3961 gattcgacct tccgggctaa cgattgttct gtggagaaaa tgtccagaag cggataaaaa
     4021 aacacccgaa caaggtggaa gtttcagatt actggcacag cgtgccggat gcggcgtgaa
     4081 cgccttatcc ggcctacaaa agtacgtaaa tttactatat tgcagagatt gcgtaggcct
     4141 gataagcgta gcgcatcagg caattttgcg ttcaaactcc cggacaagcc gggagtttgg
     4201 agtaggttat tcccactctt gcaggaaacg ctgaccatac tggtcggcta ccagcagtgc
     4261 ggcgtaaacc tgatctggcg tcgcgccgcc aggcatgttg tggatggttt caccttctgc
     4321 acatgccgct tctgccacaa ttcgcatttt cgccgggaca tcttctttaa tatccagttg
     4381 agcgagagtt attggcaaac ctaccgcatg gctaagtgca gctacggttt cgatttcctc
     4441 caccggcgcg ttttccagaa ccagctgcgt cagcgtacca aatgccactt tttcaccgtg
     4501 gtaatagtga tgcgcgtccg ggatagcggt caggccgtta tgcaccgcgt gagccgccgc
     4561 cagcccgccg ctttcaaaac caacgccgct caaataggtg ttcgcttcaa tcacgcgctc
     4621 cagcgccggg gttactacat gctgctcggc ggcaagcatc gctttttcgc cttcttccag
     4681 cagggtgttg tagcacagtt cagccagtgc cagcgccgcc tgggtgcact tgccgcccgc
     4741 catggtggtc gcgccgctac gagagcaagc acgcgcttca aaccaggttg ccagcgcatc
     4801 gccgataccc gccgctaaca gacgtgcagg tgcgccagcg acgattttgg tgtcgacaat
     4861 gaccatattc gggttatttg gcaacatcag atagcggtca aattcaccat catcggtgta
     4921 gataacagac aatgcgctgc acggtgcgtc ggtagaggcg atagtcggtg cgatcgctac
     4981 cggaacaccc atgaaatgtg ccagtgcttt ggcagtatcg agagtttttc cgccaccgat
     5041 accgagaatt gcgccacact gcgcagtctc cgcgatgcca cgcagacggt cgatctcatt
     5101 ttgcgaacat tcaccgccaa acggcgcaat ttctactacc agtccagcat ctttaaagct
     5161 tttctcgaca gtggattgag caaaacctaa aacaaatttg tcacccacca ctaaccagcg
     5221 ttctgccagc ggcttcaggt attcgcccag acgattaatc acatcagcgc cctggatgta
     5281 tttacccggt gattgaataa tgcggtccat aattgctcct ttagagatga gtagtgccaa
     5341 atgcggcatt ccagtcgtgt tcgaacttct ctatagctga ctctaccgca ggggtgttga
     5401 gcatttgttg cgctacatct aagggcaggg tgatggattc acatcctgcc agtaaacagt
     5461 ccagcgcctg acgtggtgtt ttaaagctgg cagccagcac catgctttct ggcgcatgca
     5521 tttccaacag cgcttgcagc tcctgtacag tacgaatgcc gtcaccgccc tgggcatcta
     5581 cgcggttaac gtatggagca acgtatttgg ctccagccag cgccgccagt aatccttgcg
     5641 ctgcgctgta cactgcggtt cccagcgtag taatgccttc tttcttcagc attttaattg
     5701 ctgccagacc ttcagaggtt accggaattt taaccacaat gcccggaata gcgtcgcgca
     5761 ggcgtttcgc ttctttcacc ataccctgcg cgtcgcggct catggtctga gcaaacagaa
     5821 ttccctcatc accgatcgct ttttgaaggc gcggcagcac ttcccagatg gactccttgc
     5881 tggcagcgat aatgctcggg ttagttgtca ccccggcaat cgggaatatg cgtgccagac
     5941 gttcgacttc tgcgacgtta gcggtgtcca gatacagttc catgatgttt ccttttacag
     6001 ttccagttca tgttgcagca ggctggcgat agcgtcggca gaggcggcgt taaccagcgc
     6061 gttacggaat tcttcgtgca tgatgcgacg agcgaggcgc gagaaaatgc gcatgtgctg
     6121 atcgcccgca gcatgtttgt tcagggttaa cataatgatg aattgcgctt catcatcgcc
     6181 ccagcgcacc ggcgctgcca gacgcgccac gctgatggtg gattgctcaa tgtgttctga
     6241 tttgctgtgg ggaatggcaa aactaaagcc cagaccggta gagaaaacgg cctcgcgcgc
     6301 ccacaagtcg gcttccagtt tacgcggata gcgacagcgg cccgccagca gcaggttatc
     6361 ggtcatgcct ttgagcactt cttctttgct gcgccagtcg ctttccagtg tgatgcactc
     6421 ggcggtgacc agcggtgcgt cttgttgggt catgcggaat tgcgccagca ggtgttccac
     6481 ttccagcgaa gtacggcagg ccattgcctg gttgagcaac ttgcggcact cacggctatc
     6541 aagttgcgcc atccgcgctt tcgccgccgg aattgatggt gcgctcatgc tgagttcatc
     6601 cagccctaaa ccgaccagca acggcagcac ggaacctttc gctcccagtt caccgcacag
     6661 accaatccat ttgccctggc gatgcaccgc ctgcacggcg taatcgagcg cccgcaagaa
     6721 tgctggattc aggctgttgt agtgacgagt caccttagcg ttatcgcgat ccactgccag
     6781 tagatattgc gtcaggtcat tactaccaat actaaagaaa tcaatctctt cgcagcattg
     6841 atcgatgatg aacatcaccg acggcacttc cagcatgatg ccgagtggaa tcttctcatc
     6901 aaacggaatg tgttcgttac gcagttgctg tttggcttcc gccagttttt ctttcaccca
     6961 taagatctct tccattgagg agatcatcgg gatcatgatt ttcaggctgc cgtgagcaga
     7021 ggcgcgcagg atcgaccgta gttgtgtggt aaacaacgac gcgtattctt cataaatacg
     7081 caccgcgcga taaccgagga acgggtttgc ctctgcggga atattcagat aatcaacggg
     7141 tttgtcaccg ccaatatcca tagtgcgcac aataatgctg cgtccgttgg cggactccag
     7201 cgcctgacaa aaaatgttgt acagctcgct ttcgcccggt gcactggtgc gatccatata
     7261 gagcatttca gtgcggaata aaccaacgcc ttccgcacca ttgccgaatg cggcctgcgc
     7321 ttccacggag tgagcgatgt tagcggcaat ttcaatgcgg ataccgtcag cggtacgggc
     7381 ttgttgggtc agccagacac gctgttgctc acgcagggcg tcctgtacgc gggcttcttg
     7441 ctgataataa cgggccacgg cttcccctgg ctcaaccaca atcgcccctg cgttgccgtc
     7501 gatataaatc gtttgatgct gccacggagt aagggcatca atatccacac ctaccagcgt
     7561 cggaatgttg aacgaacggg caaggatcac cgtatgtgag gtggtgccgc cacttttcag
     7621 taatagacct ttgaggtgat ttttatccag ttcgaggaac tggctggggg tcagttcatc
     7681 agccatacaa atggcgggct gcgtcagttt gcccggtgcc gggaagcgtt gctcaccgta
     7741 gatttgctgg agtaactgga agcagacgtc gcgtacgtcc agggcacgtt cttgcaggta
     7801 gctgctgctg gaacgggcga actcttcgca aaagtgattc gcactggcaa caatcgcttc
     7861 ggcgcagctt aatccggcgc tgacacctgc cagtaaatgt tcgcgcaggg aagtatcgcc
     7921 agccagggat cggtgagctt ccagtatcgc gctggtagca ccgtcgctat ccagcagacg
     7981 aaattcaatg tttttcatca ccagtgtcag gccgttttcc agcgcggatt gctcggcgtc
     8041 aacgcctttg gcagcaggaa gattacccag cgcattgaga tctaaagaag agatcggcgt
     8101 cagaatgccg cctgcactac cactgcacac ggtgcgagca cggataattt gcggattcag
     8161 attggtcagt gaaaccggca gtggttccag ttcgtcagat tttacttccg ccagcggtgc
     8221 gtcgcagtgg gggaattcat cgcgcagcca ttggcttaaa cgctggtgcg cttcctgttc
     8281 gtcggccccg gaaatcagta actggcagtt atcgcccacc agcgtatcgg tgccaatcag
     8341 cgcaagggcg cttttggcgt tgcccttgcg gtcagtgcgc aggttatgcc actcaatttg
     8401 tgatgaaaaa gtattacaca gcgtttcaac gtggcttgcc ggacgtgcat gtacgccgtt
     8461 aggtagctca caaataaatt ccacaatcag ggccattgcc tctctcccgg tacggttttc
     8521 tgtttacagg ataagagcgc acggcaacgg cctgccatgt gacaaatctg ccaaaagctg
     8581 gacaaatgta atgtaaccgt caatttgcga cgcgtctcac aagacgccgt tttgcggcat
     8641 gcttccggat catcgcaagt tatgaggcgg atcgcatttt tgtactgata ttacaaaagt
     8701 ccagtaaatg gcctttttat ccactgtttg ctccgcctgc gattgcctat tgtttcctcc
     8761 atcaaaaata tggacatggg cttttgcaag cccgggagca gcttatgaat gagttggtgc
     8821 agatcctgaa aaatacccgt cagcatttaa tgacgggcgt ttcacacatg attcccttcg
     8881 tggtatcggg cggtattttg ctggcggttt ccgtcatgtt gtatggcaaa ggcgcagtgc
     8941 cggatgccgt agccgatccg aatctgaaaa aactgtttga tatcggcgtt gcgggtttga
     9001 cgctgatggt gcctttcctc gccgcttaca ttggttactc cattgcagag cgttctgcgc
     9061 tggctccgtg cgctatcggt gcctgggttg gtaacagctt tggtgcgggc ttctttggtg
     9121 cactgatcgc cgggattatc ggcggcatcg tggtgcatta cctgaagaaa attccggtgc
     9181 ataaagttct gcgctcggtg atgccaatct tcatcattcc gatcgtcggc acactgatta
     9241 ccgcaggcat catgatgtgg gggctgggcg agcctgtggg ggcattgacc aacagcctga
     9301 ctcagtggct tcaggggatg cagcagggca gcattgttat gctggcggtg atcatgggcc
     9361 tgatgctggc gttcgatatg ggcggtccgg ttaacaaagt ggcctatgcc ttcatgctga
     9421 tttgcgttgc tcagggtgtt tataccgtgg tggccattgc tgccgtgggt atttgtattc
     9481 cgccgctggg gatggggctg gcgacgctga ttggtcgtaa aaatttctcc gcagaagagc
     9541 gcgaaaccgg taaagcggcg ctggtgatgg ggtgcgttgg ggttactgaa ggggcgattc
     9601 ctttcgccgc tgccgatccg ctgcgtgtta ttccttccat catggtcggt tcagtttgtg
     9661 gtgcagtaac tgcggcgctg gtcggtgcgc agtgctatgc aggctggggc ggtctgattg
     9721 tgctgccggt ggttgaaggc aagctgggtt atatcgcagc agtagctgtc ggagcagtgg
     9781 tgacggctgt ttgtgtgaac gtgctgaaaa gtctggcgcg taaaaatggg tcttcgactg
     9841 atgaaaaaga agacgacctg gatttggatt ttgaaattaa ttaattgagg aacgagccat
     9901 gacgaaaatt attgcagtaa ccgcgtgtcc atctggtgtt gcccatacct atatggcggc
     9961 agaggcgctg gaaagtgccg cgaaagcgaa aggctgggaa gtgaaagtag aaacccaggg
    10021 ctcgattggt ctggaaaacg aactgactgc ggaagacgtg gcgagcgccg atatggtgat
    10081 cctgaccaaa gatatcggca tcaagtttga agagcgtttt gctggcaaga ccatcgtgcg
    10141 cgtcaacatc agcgacgcgg taaaacgcgc tgatgccatt atgagcaaaa ttgaagctca
    10201 cctggcacaa accgcttaag ttcttatccc gcccgtaagg gcgggttcgc tttcccacag
    10261 gagttcctca tgacgaatcg tatctctcgc ctcaaaactg cactgtttgc caatacccgt
    10321 gaaatctcgc tggagcgtgc gctgctttat accgccagcc atcggcaaac cgaaggcgaa
    10381 ccggtgatat tgcgccgggc gaaagcaaca gcgtatatcc ttgaacatgt tgaaatttcg
    10441 attcgtgatg aagaactgat tgccggtaac cgcaccgtaa aaccgcgcgc cgggattatg
    10501 tcgccggaaa tggaccctta ctggctgctg aaagagctgg atcaattccc gacgcgtccg
    10561 caggaccgct ttgctatcag cgaagaagat aaacgtatct accgtgaaga gttgttcccg
    10621 tactgggaaa aacgttcgat gaaagatttc attaacgggc agatgacgga tgaagtaaaa
    10681 gccgcgacca gcacgcagat tttcagcatc aaccagacgg ataaaggcca ggggcacatt
    10741 attattgatt acccgcgcct gctgaatcac gggctgggtg agctggtagc gcagatgcag
    10801 caacattgtc agcaacagcc ggagaatcac ttttatcagg cagcgctgtt actgctcgaa
    10861 gcctcgcaga aacacatttt gcgttacgcc gaactggcgg aaacgatggc ggcaagctgc
    10921 actgatgccc agcgtcgcga agaactgctg actattgcgg agatctcccg ccataacgcc
    10981 gaacataagc cgcagacgtt ctggcaggcg tgccagttat tctggtacat gaacatcatt
    11041 ctgcaatacg aatccaacgc cagttcacta tcgttggggc gcttcgacca gtatatgttg
    11101 ccgttctatc aggcatcatt aacccagggc gaagatccgg cgttcctgaa agaactgctc
    11161 gaatctttat gggtgaaatg caacgacatc gtgctgttgc gctccaccag tagcgcgcgt
    11221 tatttcgccg gtttcccgac cggctatacc gcactgctcg gcgggttaac cgagaacgga
    11281 cgtagcgcgg tgaacgtgct ttcgttcctt tgccttgacg cttatcaaag cgtgcaatta
    11341 ccgcaaccga acctcggcgt gcgcactaac gccttgatcg acacgccgtt cctgatgaaa
    11401 accgcagaaa ccattcgcct cggcaccggt attccgcaaa tctttaacga tgaagtggtg
    11461 gtgccagcgt tcctcaaccg tggcgtttcg ctggaagatg cacgcgacta ttccgtagtg
    11521 ggctgtgtgg aattatctat tcccggcaga acctacggct tgcatgacat cgcgatgttt
    11581 aatctgctga aagtgatgga aatctgcctg catgaaaatg aaggcaatgc tgcgctgact
    11641 tatgaaggtt tactggagca gatccgcgcc aagatcagcc actacatcac cctgatggtt
    11701 gagggcagca atatttgcga tatcggtcac cgtgactggg cacctgtacc gctgctctca
    11761 tcgtttatca gcgattgtct ggaaaaaggc agcgatatta ccgatggcgg cgcgcgttat
    11821 aacttctccg gcgtacaggg gatcggtatc gccaacctga gcgattctct ccatgcgttg
    11881 aaagggatgg tttttgagca acagcgttta agttttgacg aattgctgtc ggtattaaaa
    11941 gccaacttcg caacgccaga aggcgaaaaa gtccgtgctc gcttaattaa ccgcttcgag
    12001 aaatacggta acgatatcga cgaggtggat aacatcagcg ccgaactgtt acgccactac
    12061 tgcaaagaag tggaaaaata ccagaacccg cgcagcggct acttcacgcc gggatcgtat
    12121 accgtttctg ctcacgtccc gttgggatcg gtggttggcg cgacgccaga cggtcgtttt
    12181 gccggggaac aactggcaga cggcggcttg tcacccatgc tgggccagga cgcacaaggg
    12241 ccaacggcgg tactgaagtc agtcagtaag ctcgataata cgctgctgtc taacggtacg
    12301 ttgctgaacg tgaaattcac tccggcgact ctggaaggtg aagcgggatt acgcaaactg
    12361 gccgacttct tacgggcgtt tacccagctt aagttgcagc atattcagtt taacgtggtg
    12421 aacgccgaca cgttgcggga agcgcaacag cgcccacaag attatgccgg gctggtggtg
    12481 cgcgttgccg gatacagcgc cttctttgtc gaattgtcga aggagatcca ggatgacatc
    12541 atccgccgga cagcgcatca gctgtaacgt tgtggaaacg cgccgcaatg atgtggcgcg
    12601 cattttcaac attcagcgtt attcactgaa tgacggcgag ggcattcgta cggtggtctt
    12661 ttttaaaggc tgtccgcatc tttgcccatg gtgtgctaat ccggagtcga tctccggcaa
    12721 aatccagacg gtacgcagag aggcgaaatg tctgcactgt gcgaaatgtt tgcgtgatgc
    12781 ggatgaatgc ccctccgggg cgtttgaacg gattggccgc gatatcagcc ttgacgcgct
    12841 ggagcgggaa gtgatgaaag atgacatttt ctttcgcacg tccggcggcg gcgtcacgct
    12901 ttctggcggc gaagtgttaa tgcaggcgga gtttgctacc cgttttttac agcggctgcg
    12961 gttgtggggc gtgtcatgcg ccattgaaac tgccggagac gcgccagcca gcaagctgtt
    13021 accgctggcg aaattgtgcg atgaagtgtt gttcgattta aaaattatgg acgtgactca
    13081 ggcgcgggat gtggtgaaga tgaatctgcc acgcgtgctg gagaatctgc gtttgctggt
    13141 gagtgagggc gtcaacgtga tcccgcgttt accgctgatc cctggtttca cgctcagcca
    13201 ggagaatatg cagcaggctc tggacgtgct gatcccgctg aatatcaggc agatccatct
    13261 gttaccgttt catcagtacg gcgaaccgaa ataccgcctg ctggggaaaa catggtcgat
    13321 gaaagaggtg cctgcgccgt cgtcagctga tgtggcaacg atgcgcgaaa tggcagaacg
    13381 ggccggattt caggttaccg tgggaggtta aaatggcata cctggtggca gtaaccgcct
    13441 gcgtcagtgg cgtggcgcat acttatatgg cggcggaacg gctggaaaag ttgtgcctgt
    13501 tagagaagtg gggcgtcagc attgaaactc agggcgcgct gggaacggag aatcgtttag
    13561 cggacgagga tatccgccgg gcggatgttg cgctgttaat tacggatatc gagcttgccg
    13621 gtgctgagcg atttgaccat tgccgctatg tgcaatgcag catctacgct ttccttcgtg
    13681 agccgcagcg ggtgatgagc gcggtacgca aagtgctttc tgcgccgcag caaacccatc
    13741 tcattctgga gtagtcggtt tttctgtcag ttggctgtgg tactgccgac gatattccga
    13801 cggtgagcgt tcggtatttt tacgaaacag acggcagaag tagttgctgt cgacaaagcc
    13861 gcaggcgtgc gccacttctt ttaccttcag gtcatagcct ttcaataacg tcttagcgtg
    13921 ctccagtcgc gtgtgattca ggtattcgtt aaaaccagtg gcccccgttt tttgaaacag
    13981 gtgcgagagg taatttggcg aaatatagaa tgcctgtgcg acagattcgc gggtaagcgc
    14041 ggaggcatag cgttcgtcga tataatcacg aatagcttcg aacagtgcct ggctgcgtga
    14101 ggcggtctgg atttggctgc caagcaaatc gcggcagtgg ctaagcaggc tggcgacgat
    14161 aagccttgcc gtttgctgct cctgcgactg catttgcatt tcattgagcg tttgtagcag
    14221 aaaagaacct atgcgtgggc cgcgtcgggc gacgtgttgc ttcgccagat tttgatattg
    14281 tttgccatcc cattgcacga cgctgaagcc gagttgctgt ttgccaaaca gcacgctaaa
    14341 ggtagtggcg ggggcttgcc attgtggaaa attccagcca ccagcgggaa cgtacagcac
    14401 atcgccggga actaacgctg ctccagcgcc ggtatcaata aactcacctt ccagcacaat
    14461 ttccagccgt ggaaaatcga cctgatacgc aagatcgggc accgggccgt tgctgctggc
    14521 aaagtaaatc tggcgcaggg acagcgggcc gttgatcagg cgggagagca gatagctgac
    14581 gtcgtgatac atgttattcc cttaggtatg tagacgggaa aacagtaact ggcattatga
    14641 aaaaaggcca cctgaaaagt gtggcctgat gggagtgaga tgtaaacatt gcggcagttt
    14701 cagtcgactt gtattgccgg atgcggcgta aacgccttat ccggcctacc gccgcgatct
    14761 gtaggccgga taagacgcgt caagcgtcgc atccggcaaa taacatctgg ctgataatta
    14821 ctgattaccc acctgatcgc catacggcag agtgtcgtaa tcgatcagtg cgtttttctt
    14881 atacgggtta ccaatccagc gggtagtttc tttgaactgc ggattcacca ctgaacgggt
    14941 tggatcgtaa ccgtcgtaag ataagcccgc caaatctgac caggtgtgga tcagttccgc
    15001 caggctgtat ttacgatcaa catcctgtga gaaatcacgc ggatgagtcg cttgccattt
    15061 ttctgacgtc cacagcagga acggaatggt gtacatatga cgcgtcgggt tgtcttcatt
    15121 acgcccctga gttttgtgcg gcggcgtgtc gtaaacctct tcaccgtggt cagagaaata
    15181 aaccaggaaa ccgttcggat cggtcgcttt aaagtcttta atcaggctgg caaccacgtg
    15241 atcgttgtac aggttagcat tgtcataatc attatatgac tccagctctt ctgcgtttaa
    15301 tcccggtgga acatgctctg tattaccgtc aaatttgccc tgatcttccg gataacggta
    15361 cttgtacttg atatgcgtac ccagcagatg aacgatgatc agtttcttcg gcgcagggtc
    15421 cttcagcact tcctggaacg gcttcagcac gttggtgtcg tattcacgcg cactctgcgt
    15481 acgttgctgg ttcatgtagt actgcttatc ggtctggcgc gaaaataccg tcagcatggt
    15541 attgcgggcg gtcatcgtct gctggttggt gatccagaag gttttataac ccgcctgttt
    15601 catcatgttc atcagcgacg gctgcgtcag atacagatcc gggttctttt cattggcaaa
    15661 ggtcagcgcc tgttgcagga tttcaatggt gtacggacga gacgtaacca cgttattaaa
    15721 cacggtcaga ttcggatcgg ttttatgcag cgcatccagc tccggcgtgg tttcacgcgg
    15781 ataaccgtac agactcatgc gtccacgctg ggtcgattcg ccaattacca gcactaaggt
    15841 gcgcggttcg ttacccgatt catctttgaa attcgccagc ggcggcaagg cattattttc
    15901 attcagtagc tttgttagcg agtttagttg ctgacgatac tgataatagc cggtcaggaa
    15961 ttgccacggt gcggcaggct ccatacgcga tgccaggtta tccaacgttt tctcaaatgg
    16021 cttgtttttg ataaacgtat tcatggcgat cggatgcaga atcaagccat aaagtagggc
    16081 aaaagagaca acataacgcc acggctttgg aatatagacc gggcgcaggc gcgtccacag
    16141 caaaactgcc accgccgtat aggccagcgc gataagcaca atcttcaggc tgaaatactg
    16201 gcttaaatac tcgctggctt cgttggtgtt ggtttcaaac atcacaaaca gaacgctctg
    16261 cgagaactcc tgaccgtaga tgacgtagta gcacaacgct gccagagagg ccgcccatag
    16321 caccacgccg atcactgcgg caataatttt aatccgcttc ggaaagagga ataccgggat
    16381 caaccacagc gaactgaata acagcgagtc gcgaatgccg ttagtgccac tataaccact
    16441 gatgtaaata atggcctgta gcagagtaga gaaaaaccaa aagtagagca gtgcccaacc
    16501 cagggccttc cagctaaaaa ggggtttagc ctggacttct gtggaatgca tagtaagaac
    16561 ctgtcttgaa aaaaatatcg ccgaatgtaa cgacaattcc ttaaggatat ctgaagggat
    16621 atccagattt tgaataaaat gcagacagaa atatattgaa aacgagggtg ttagaacaga
    16681 agtatttcag aaaaccctcg cgcaaaagca cgagggtttg caggagagga agattagccg
    16741 gtattacgca tacctgccgc aatcccggca atagtgacca ttaacgcctg ctcgacgcga
    16801 ggatccggct catggccttc tttttctgcc tggcgggagc ggtgcagcaa ctcggcctgc
    16861 aatacgttca gcgggtcggt gtaaatattc cgtagctgaa tagactctgc aatccacggc
    16921 agatcggcca tcaggtgaga atcgttggca atcgccagca ccactttgat gtcttcttct
    16981 tgcagattgc gtaactcttt acctaacggc cacagtgctt tgtctaccag gcgttggtca
    17041 tagtattccg ccagccacag gtctgctttg gcgaagacca tctccagcat gccgagacgc
    17101 gtcgagaaga atggccaatc gcggcacatg gcttccagct cgctctgttt gccatcttcg
    17161 accacttttt gcagcgccgt acctgcaccc agccaggcgg ggagcatcag gcggttttgt
    17221 gtccaggcaa aaatccacgg aatagcgcgc agtgactcaa cgccaccggt cgggcgacgt
    17281 tttgccggac gtgaacccag cggtagtttg ccgagttcct gctccggcgt ggcagagcgg
    17341 aagtaaggca caaaatcttt gttttcacgc acgtagccgc ggtagacatc gcatgagata
    17401 accgaaagct catccataat gcgacgccag ctctctttcg gctctggcgg tggcagcagg
    17461 ttggcttcca gaatcgcccc ggtatacagc gaaaggctgc taacggtgat ttcaggcaga
    17521 ccgtacttga agcggatcat ctcgccctgt tcggtcacgc gcaggccgcc tttcaggctt
    17581 cctggtggtt gtgacagcag cgccgcatgg gcaggtgcgc cgccgcgacc aatggaaccg
    17641 ccgcgaccgt ggaacaacgt tagctcaata cccgcttttt cgcaggtttt gattaatgca
    17701 tcctgtgcct gatattgcgc ccaggaagct gccatcacgc ccgcatcttt tgctgagtcg
    17761 gaatagccaa tcatcaccat ctgtttgccc tgaatcaggc cgcgatacca gtcgatattc
    17821 agcagctggg tcatgacatc gttggcgttg ttcaggtcat cgagggtttc aaacagcgga
    17881 gcaaccggca tcgcaaaccc gatacccgct tctttcagca gcaggtggac agccagcacg
    17941 tcggacggcg ttttcgccat cgagatcacg taggcggcaa tggagccttg cggtgcttcg
    18001 gcaatcacct ggcaggtatc gagcacttcg cgcgtttcgg cgcttggttg ccagttgcgc
    18061 ggtagaagcg gacgtttgga gttcagttcg cggatcagga acgcctgttt gtcggcttct
    18121 gaccagctct cgtagtcgcc gataccgagg tagcgggtca gctcgcccag cgcttcggta
    18181 tggcgggtgc tctcctgacg gatatcaata cggaccagcg gtacgccgaa acatttcacg
    18241 cggcgcaggg tgtcgagcag atcgccgttg gcgataatac ccatgccaca cgcctgaagt
    18301 gactggtagc aagcgtagag cggttcccac agttcttcgt tttgtgtcag caggccttct
    18361 ggttttggca gttcttcgcc tttcaggcgc gcttccagcc atgcctgtgt cgccatcagg
    18421 cgagaacgca ggtttttcat cagatagcga tacggttctg cggcaccttc ttcgccaacc
    18481 agcgccagca gttcaggggt cgcttcaacc atcgacagtt cagaaaccag cacctgaata
    18541 tctttcagga acagatcggt ggctttccag cgactgagca gcaggacgtg gcgggtgata
    18601 tcggcagtga cgttcgggtt gccgtcgcgg tcaccgccca tccacgaggt aaaacggacc
    18661 ggaacaaatt cgacgggcag tttgtagccg agattctctt ccagttgttc gttcagttcg
    18721 cgcaggtaat tcggtacgcc ttgccacagg ctgttttcta ctacggcaaa gccccatttg
    18781 gcttcatcta ccgggcttgg acgcagctta cggatttcat ctgtatgcca tgactgggcg
    18841 atcaactggc gcaggcggcg catcagctgg ttatgttcgt agtcggcgat atctttgtta
    18901 tcgagctgtt ttaagcaggc gttcacttcc accattttgt ggatcagtgt acgacgggta
    18961 atttcggttg ggtgagccgt gaggaccagt tccagcgaca gcgattccac tgcttttttg
    19021 atggtgtctt cgctcagttc cggctggttt ttcagtttac gcagggtgcg ggcgatcact
    19081 tccgggttgc tggcagcttc gcctttcggc gaaatgctgt ggtattgctc agcggtgttg
    19141 gccaggttca ggaactgact aaacgcacgc gcaacgggca gcagctcgtc gttcgacaaa
    19201 ttttgtaagg tggtgagcaa ctcctggcgg ttagcatcat tgccagcgcg tgaagatttc
    19261 gacaacttac ggatagtttc tacgcgttca agaatgtgtt ctcccaacgc atccttgatg
    19321 gtttctccca gcactttgcc gagcatactg acattactac gcaatgcgga atattgttcg
    19381 ttcatattac cccagacacc ccatcttatc gtttgatagc cctgtatcct tcacgtcgca
    19441 ttggcgcgaa tatgctcggg ctttgctttt cgtcgtcttt tataaagcca cgtaaaagcg
    19501 gtgacgtcaa atgctgcgaa atcgcttcag caaacgaata aatagcagga atttacgcca
    19561 ttaaattcat gacgctttaa ataagcgtaa cttatgggaa tgttaaaaaa tcgcctcgag
    19621 taacaccaaa ggtgtaggtc ggataagatg cgtaagcatc gcatccgaca ttgtttggcc
    19681 tacgttttaa tgccagcaaa aatggtgaat cacctgggtg atcagttcgc gggtgggctt
    19741 gataaacctc gtctccagat attcatcagg ctgatgagcc tgattaattg agccaggccc
    19801 cagaaccagc gtcgggcata acgtttgaat aaacggcgct tcggtacagt aattcaccac
    19861 ttcggtcttt gctccgagca atttctcaac cacttcaacc agtttatgat tcggtgggca
    19921 ttcatagcca gggatcggcg gatgcagctc gtcgaccgtc agacgacccg gccagcgttc
    19981 gctcactgga gccaatgcat cgttgagcaa accattaagt tcattgagtg tcatgcctgg
    20041 cagcggacga atatccatat gcaactcaca gcaagcacaa atacggttag aagcgtcgcc
    20101 accgtggata tgcccgaggt tgagcgtagg gtatggcacg gtaaacgcgt cgtagtgata
    20161 acgttctttc aggttatcgc gcaattgcaa aatatgcccg atggcgtcgt gcatcagttc
    20221 gatagcgtta actccgcgtg ctggatcgct ggagtggccc gactggccct gaatacggat
    20281 ggcgttagag atatgacctt tatgcgcgcg taccggttgc agcgacgttg gctcgccaat
    20341 gatggcgcaa tccgggcgca gggcggtagt ttcggcaaaa taacgcgctc cggccatact
    20401 ggtttcttca tcggcagtcg ccagaatgta gagcggtttt gcaagcttcg tgacgtcgac
    20461 atcgcgtagc gcatcaagga taaacgcaaa aaagcctttc atgtcggcgg tgcctaagcc
    20521 gtaaagcttg ccgtcatgct ccgtcaacgt aaatggatcg cgcgtccagc gtccgtcatc
    20581 aaatggcacc gtatcggtat gccccgccag caacaaaccg ccagccccct gtccgcaact
    20641 ggccagcata ttaaatttgt tgcgagttcc tggcacaggc tgtacttcca cattgaagcc
    20701 taaatcctta aaccagtccg ccagcagagt gattaaatct gcattgcttt gatcgagtgc
    20761 ctcttccgtg gcgcttattg aaggtgtggc aatcagagca cggtaaatct cgataaatgg
    20821 cggtaatttg tttttcattg ttgacacacc tctggtcatg atagtatcaa tattcatgca
    20881 gtatttatga ataaaaatac actaacgttg agcgtaataa aacccaccag ccgtaaggtg
    20941 aatgttttac gtttaacctg gcaaccagac ataagaaggt gaatagcccc gatgttgaat
    21001 acgctgattg tgggtgccag cggctacgct ggcgcagagc tagtgaccta tgtaaatcgc
    21061 catccgcata tgaacataac cgctttgact gtttcagcgc aaagcaatga tgcgggaaag
    21121 ttaatctccg atttgcatcc gcagctaaaa ggcatcgttg atctgccgtt gcagccgatg
    21181 tcggatatca gcgagtttag cccaggggtg gacgtggtgt ttctcgccac cgcccatgaa
    21241 gtcagccacg atttagcgcc gcaatttctt gaagcaggct gtgtggtgtt cgacctttcc
    21301 ggcgcgtttc gtgttaacga tgtcgccttc tatgaaaaat attacggctt tacccatcaa
    21361 tacccggaac tgctggaaca ggcagcctac ggtctggcgg agtggtgcgg taataaatta
    21421 aaagaagcga acttgattgc cgtgccaggc tgttatccga cggcagcaca gctggcgctg
    21481 aaaccgttga ttgatgccga tcttcttgac ctcaatcagt ggccggtgat caacgccacc
    21541 agcggcgtga gcggtgcagg acgtaaggca gcgatttcaa atagtttttg tgaagttagc
    21601 ctgcaaccgt atggcgtctt tacccatcgc catcaaccag agatcgccac acacctcggt
    21661 gctgacgtta tcttcacccc gcatctgggc aatttcccgc gcggcattct cgaaaccatt
    21721 acctgccgcc tgaaaccggg tgtgagccag gtgcaagtgg cgcaagcgtt acagcaggcg
    21781 tatgcccata aaccactggt gcggctgtat gacaaaggcg ttccggcgct gaaaaatgtc
    21841 gttggactgc cattctgcga tatcggtttt gccgttcagg gcgagcatct gattattgtg
    21901 gcgaccgaag acaacttact gaaaggcgcg gcggcacaag cggtgcagtg cgccaatatt
    21961 cgtttcggct atgcggaaac gcagtctctt atttaagggt gcaatgatga atccattaat
    22021 tatcaaactg ggcggcgtac tgctggatag tgaggaggcg ctggaacgtc tgtttagtgc
    22081 actggtgaat tatcgtgagt cacatcagcg tccgctggtg attgtccacg gtggcggctg
    22141 cgtagtggat gagttgatga aagggctgaa tctgccggtg aaaaagaaaa atggcctgcg
    22201 ggtgacgcct gctgatcaga ttgacattat caccggagca ctggcgggaa cggcaaataa
    22261 aaccctgctg gcatgggcga agaaacatca gattgcggcc gtaggtttgt ttctcggtga
    22321 cggcgacagc gtcaaagtga cccagcttga tgaagagtta ggccatgttg gactggcgca
    22381 gccaggttcg cctaagctta tcaactcatt gctggagaac ggttatctgc cggtggtcag
    22441 ctccattggc gtaacagacg aagggcaact gatgaacgtc aatgccgatc aggcggcaac
    22501 ggcgctggcg gcaacgctgg gcgcggatct gattttgctc tccgacgtca gtggcattct
    22561 cgacggcaaa gggcaacgta ttgccgaaat gaccgccgcg aaagcagaac aactgattga
    22621 gcagggtatt attactgacg gcatgatagt gaaagtgaac gcggcgctgg atgctgcgcg
    22681 cacgttgggc cgtccggtag atatcgcctc ctggcgtcat gcggaacagc ttccggcact
    22741 gtttaacggt atgccgatgg gtacgcggat tttagcttaa gttttgttgg ccggaggcgc
    22801 agctttccgg cattgaattt caaaataagg aaacagagtt atggcacttt ggggcgggcg
    22861 ttttacccag gcagcagatc aacggttcaa acaattcaac gactcactgc gctttgatta
    22921 ccgtctggcg gagcaggata ttgttggctc tgtggcctgg tccaaagccc tggtaacggt
    22981 cggcgtgtta accgcagaag agcaggcgca actggaagag gcgctgaacg tgctgctgga
    23041 agatgttcgc gccaggccac aacaaatcct tgaaagcgac gccgaagata tccatagctg
    23101 ggtggaaagc aaactgatcg acaaagtggg tcagttaggc aaaaaactgc ataccgggcg
    23161 tagccgtaat gatcaggtag caactgacct gaaactgtgg tgcaaagata ccgttagcga
    23221 gttgctgacg gctaaccggc agttacaatc cgctctggtc gagaccgcgc agaacaatca
    23281 ggacgcggtg atgccgggtt acactcacct gcaacgcgcc cagccggtga cgttcgctca
    23341 ctggtgcctg gcctacgttg agatgctggc gcgtgatgaa agccgtttgc aggacgcgct
    23401 taagcgtctg gatgtcagcc cgctaggctg tggcgcactg gcaggaacgg cctatgaaat
    23461 cgaccgtgaa cagttagcag gctggctggg ctttgcttcg gcgacccgta acagtctcga
    23521 cagcgtttct gaccgtgacc atgtgctgga actgctttct gctgccgcta tcggcatggt
    23581 gcatctgtcg cgttttgctg aagatctgat tttctttaac accggcgaag cggggtttgt
    23641 ggagctttct gaccgtgtga cttccggttc atcattaatg ccgcagaaga aaaacccgga
    23701 tgcgctggag ctgattcgcg gtaaatgcgg tcgggtgcag ggggcgttaa ccggcatgat
    23761 gatgacgctg aaaggtttgc cgctggctta caacaaagat atgcaggaag acaaagaagg
    23821 tctgttcgac gcgctcgata cctggctgga ctgcctgcat atggcggcgc tggtgctgga
    23881 cggcattcag gtgaaacgtc cgcgttgcca ggaagcggcg cagcagggtt acgccaacgc
    23941 tactgaactg gcggattatc tggtggcgaa aggcgtaccg ttccgcgagg cgcaccatat
    24001 tgtgggtgaa gctgtggtgg aagccattcg tcagggcaaa gcgttggaag atctgccgct
    24061 cagtgagttg cagaaattca gccaggtgat tggcgaagat gtctatccga ttctgtcgct
    24121 gcaatcgtgc ctcgacaagc gtgctgcaaa aggcggcgtc tcaccgcagc aggtggcgca
    24181 ggcgattgct tttgcgcaag ctcggttaga gtagtttgtt tatattgctc ccctgaaaat
    24241 ggggagctta ccacgcctac aaagtgcgac acttcgtagg cggataaggc gtttacgccg
    24301 catcaggcag aaatttgtca gtattctggt gaccttaaat ttaacgccca accagaacca
    24361 ttatttgtca cattcctttt tattcttgcg tcttctttac cttcactctc cccataaaaa
    24421 ttatattttc aatacatcag cc
//
LOCUS       DAZUCC010000058        24313 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZUCC010000058 DAZUCC010000000
VERSION     DAZUCC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.058"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(130..1011)
                     /gene="prmA"
                     /locus_tag="ACHY09_002011"
     CDS             complement(130..1011)
                     /gene="prmA"
                     /locus_tag="ACHY09_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS3884989.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1023..2474)
                     /gene="panF"
                     /locus_tag="ACHY09_002012"
     CDS             complement(1023..2474)
                     /gene="panF"
                     /locus_tag="ACHY09_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFS3884990.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRTVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2464..2706)
                     /gene="yhdT"
                     /locus_tag="ACHY09_002013"
     CDS             complement(2464..2706)
                     /gene="yhdT"
                     /locus_tag="ACHY09_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFS3884991.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2815..4164)
                     /gene="accC"
                     /locus_tag="ACHY09_002014"
     CDS             complement(2815..4164)
                     /gene="accC"
                     /locus_tag="ACHY09_002014"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS3884992.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4175..4645)
                     /gene="accB"
                     /locus_tag="ACHY09_002015"
     CDS             complement(4175..4645)
                     /gene="accB"
                     /locus_tag="ACHY09_002015"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS3884993.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5050..5437)
                     /locus_tag="ACHY09_002016"
                     /pseudo
     CDS             complement(5050..5437)
                     /locus_tag="ACHY09_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            complement(5623..6597)
                     /gene="yhdH"
                     /locus_tag="ACHY09_002017"
     CDS             complement(5623..6597)
                     /gene="yhdH"
                     /locus_tag="ACHY09_002017"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFS3884994.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVTLLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            6749..8689
                     /gene="csrD"
                     /locus_tag="ACHY09_002018"
     CDS             6749..8689
                     /gene="csrD"
                     /locus_tag="ACHY09_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFS3884995.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8994..10037
                     /gene="mreB"
                     /locus_tag="ACHY09_002019"
     CDS             8994..10037
                     /gene="mreB"
                     /locus_tag="ACHY09_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFS3884996.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10103..11206
                     /gene="mreC"
                     /locus_tag="ACHY09_002020"
     CDS             10103..11206
                     /gene="mreC"
                     /locus_tag="ACHY09_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS3884997.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11206..11694
                     /gene="mreD"
                     /locus_tag="ACHY09_002021"
     CDS             11206..11694
                     /gene="mreD"
                     /locus_tag="ACHY09_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS3884998.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11703..12296
                     /gene="yhdE"
                     /locus_tag="ACHY09_002022"
     CDS             11703..12296
                     /gene="yhdE"
                     /locus_tag="ACHY09_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFS3884999.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12286..13755
                     /gene="rng"
                     /locus_tag="ACHY09_002023"
     CDS             12286..13755
                     /gene="rng"
                     /locus_tag="ACHY09_002023"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFS3885000.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13823..17623
                     /gene="yhdP"
                     /locus_tag="ACHY09_002024"
     CDS             13823..17623
                     /gene="yhdP"
                     /locus_tag="ACHY09_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFS3885001.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGQVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTH
                     GDSTNSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17779..19224
                     /gene="tldD"
                     /locus_tag="ACHY09_002025"
     CDS             17779..19224
                     /gene="tldD"
                     /locus_tag="ACHY09_002025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFS3885002.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19352..20281)
                     /gene="aaeR"
                     /locus_tag="ACHY09_002026"
     CDS             complement(19352..20281)
                     /gene="aaeR"
                     /locus_tag="ACHY09_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFS3885003.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20464..20667
                     /gene="aaeX"
                     /locus_tag="ACHY09_002027"
     CDS             20464..20667
                     /gene="aaeX"
                     /locus_tag="ACHY09_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFS3885004.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20675..21607
                     /gene="aaeA"
                     /locus_tag="ACHY09_002028"
     CDS             20675..21607
                     /gene="aaeA"
                     /locus_tag="ACHY09_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFS3885005.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21613..23580
                     /gene="aaeB"
                     /locus_tag="ACHY09_002029"
     CDS             21613..23580
                     /gene="aaeB"
                     /locus_tag="ACHY09_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFS3885006.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23672..23944
                     /gene="yhcO"
                     /locus_tag="ACHY09_002030"
     CDS             23672..23944
                     /gene="yhcO"
                     /locus_tag="ACHY09_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFS3885007.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23999..24262)
                     /gene="yhcN"
                     /locus_tag="ACHY09_002031"
     CDS             complement(23999..24262)
                     /gene="yhcN"
                     /locus_tag="ACHY09_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFS3885008.1"
                     /translation="MKIKTTVTALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5764 a         6140 c         6588 g         5821 t
ORIGIN      
        1 taagttctca atttttcttt atgaatgaaa attttggcac gcaaatttgc gtaaataatc
       61 ggcaaattaa cgtgcctttt gtgacatcgc tcgcacttcg ctctgcgtca ccctacggcg
      121 atgcgaaggt taattcttac gaccggtaat gcggcaccac tcttcttttt ccacgaccgg
      181 gtccagagcg aagctgtcgg cataagcttc gcaaacgctc tctgcctggc ttgccagaat
      241 accggaaagg cccagcaaac cgcctgaaac tggcaggacg ctgattaacg gtgccagttc
      301 acgtaatggg cctgcaagga tgttagcgac caccacgtcg gctttcattt cttctggctg
      361 atctttcggt aagtagagtt ccagacggtc agagacgcca ttacgttccg cgttgtcgcg
      421 gctggcctga atcgcctgcg gatcaatatc aataccaatg gcttttgctg cacccagttt
      481 cagcgccgcg atagccagaa tgccggaacc gcagccaaag tcgatgactg ttttaccggt
      541 taaatcgagg ctgtcgagcc actgcaggca cagagaggta gttggatggg tacccgtacc
      601 aaacgccagc cctggatcta acatcacgtt gacggcgttt tcgtccggca catcacgcca
      661 gcttgggcag atccacagcc gttcaccaaa gcgcatcggg tggaaattat ccatccattc
      721 gcgctcccag tctttatctt ccagttgttc gattttatgc gcgaagcccg cgccgagcag
      781 cggatggttt tccagaatcg ccaccacgtc gttcatatcg gtttcagcgt cgaacagacc
      841 aatcacatcg gtgtcgcccc acaggcgcgt ttcgcccggc agcggctcaa acacaggcgt
      901 atcgtgggta tcctgaaaag tgatagaaac ggcacccgct tccatcagcg catcgctaag
      961 atcttccgcg ttcgcgccgg tggtgttcag tttcagttgg atccaaggca tggcaaaact
     1021 ctttatttat cagtagtcaa aacggtagct tgcgggacgg atgtaccgaa acggtttccg
     1081 accaggaaag ccagcaaact tagtagtaac gagggcacga tagggtggaa gcccaggtac
     1141 tgaatattca gcgtcgcgag tacggcatac agtacgccgc caacaatcat cgcacttagc
     1201 gcgcctttgg cgttggcgcg ttcccagtaa agacccagta ccagcggcca caggaaaacg
     1261 gcttccagcc caccgaaggc cagcaaattc agccagatga tcatttctgg cggcttccag
     1321 gcggcaagca gcagcaacgc accgagaact aacgtaatta ccgccgacat ccgcttcaga
     1381 cgcgtctcgt tttgcatttg atccggacgg atattcagat agagatcttt aatgatcgta
     1441 gcggaacttt gcagcagttg ggcgttaatt gtcgacatga tcgcagccat aggtgcagcc
     1501 aggaagatcc cggcagcaaa cggtggcaac acttttacca ttaacgtcgg gatcaccagg
     1561 tccggtacgg tgagatcggg gatcaccgtc cgacctaacg ctccggccag gtgcataccg
     1621 aacatcagga ttgcgaccac aatcgtaccg atgatgatcc cccgatgtac ggctttgctg
     1681 tctttataag agatacagcg caccgcagta tgcggcaggc caatcacgcc aaagcacacc
     1741 agtacccaga acgacgtcat aaaggcaggc gacagaatat cgtcagcgcc ttgtggcgta
     1801 accagttgcg gatcgatggt ttgcaacgtc tctactgcgt tgcttaagcc acctgcggca
     1861 tgtaccacgc caataagcag cacaatggtg ccaatcagca tcaccagccc ttgcatagtg
     1921 tcgttcagca cgctggcgcg aaagccacca aaggcggtat acaacgcaat gctgataccg
     1981 aaaatcagca gcccggtttc ataaggaata cccgccgcgg tttccagcag gcgcgcgccg
     2041 ccgataaact gcacggtcat cgcgccaata aacgcgacca gcaaactcaa actcgccagc
     2101 cacaccaaaa gacgactctg gtagcgggca aacagcatgt cgttcagtgt caccgcattg
     2161 tagcggcgcg caagaattgc gaacttcttg ccgagaatac cgagcgaaag ccagactgca
     2221 ggaagctgaa tcatcgccag caatacccag cccaggccgt atttataagc agctccaggc
     2281 ccgccgataa acgaactggc actgatatag gtcgcggtga gcgtcatcgc cagcacaata
     2341 ccgcccatag agcggctgcc gaggaaatac tcattaagga aggtgccagt gctccgttta
     2401 cgcatcgcat aaacagagat accgaacacc accaccagat aggcgaccag cggtagaatt
     2461 acttcaagct gcatcgtcat cctccagtgg gatatcgcga tagataaatt tcaccatcgc
     2521 ccagcacagt ccaataaaca gcagcggcgt caggatgcag gccatctcaa accagcgcgg
     2581 aaaaccggta aaaccggggg caacgccagg taagtaagcg gctactaacc ataccgccag
     2641 atacaaaagg gtcagcccca gcgcccagcg cgcctcttta tgggcctgaa caaaacgagt
     2701 gtccattttt tgtccctgat gggtgaagaa agcggggatt gtaccttatg ggggttgtcg
     2761 atccccagta ataaaaaagg ccggaaaatc cggccttttg acgcttttgc agtcttattt
     2821 ttcctgaaga ccgagttttt tctccagata gtggatgtta gtgccaccat gctggaagtt
     2881 ctcgtcattc atgatgcgga tctgcagatc aacgttggtt ttgataccgt cgatgatcag
     2941 ctcctgcagc gcattcttca tgcgggcaat cgccacgtca cggttttcac cgtagcaaat
     3001 cagcttaccg atcattgagt catagtacgg cggtacggtg tagcccgcgt agatatgaga
     3061 ctcccaacgc acgccaaaac cgcctggtgc gtggaaacgg gtgattttgc ccgggcttgg
     3121 caggaaggtg ttcggatctt cggcgttgat acggcattcc accgcatggc cgcgaacgtg
     3181 aacttcttct tgcttgatcg acagcggttg accggcagcg atacgcagct gttctttgat
     3241 caggtcaacg ccggtgatca tttcagtaac cgggtgttct acctgaatac gggtgttcat
     3301 ttcgatgaaa tagaactcgc cgttttcgaa caggaactcg aaagtacccg caccgcgata
     3361 gccgatatca acacatgctt tagcgcaacg ttcgccgatg tagcgacgca gttccggggt
     3421 aatgcccggt gctggtgctt cttcgaccac tttctggtgg cggcgctgca tggagcagtc
     3481 acgttccgcc agatagatag cgttgccctg accgtcagcc agtacctgaa tctcgacgtg
     3541 gcgaggattt tccaggtatt tctccatgta aaccatatcg ttgctgaaag cagctttcgc
     3601 ttccgcacgg gtcatggaga tggattgtgc cagttcagcg tcgccgcgca ctacgcgcat
     3661 accgcgaccg ccgccgccgc cggaggcttt gataattacc ggataaccaa tgcgtttagc
     3721 aatggcgcgg tttttatcca tatcgtcgcc cagcgggccg tcagaacccg gtacgcaagg
     3781 aacgcccgct tttttcatcg ccgcgattgc ggatactttg tcgcccatca ggcgaatggt
     3841 ttctgctttc gggccgatga agataaagcc ggagcgttca acctgctcgg caaagttggc
     3901 gttctcggag aggaagccgt aacccggatg gattgctact gcgccggtga tttcagcggc
     3961 gctgatgatt gccgggatgt tcagataact ttttactgac ggagcagggc cgatacagac
     4021 cgtttcatct gccagtaata cgtgttttag atcgcgatcc gcgctggagt gcacagcgac
     4081 agtcttgatg cccagttctt tacaggcacg aagaatacgc aatgcaatct cgccgcggtt
     4141 ggcaataaca attttatcca gcatgttcgc ctcgttactc gatgacgacc agcggctcgt
     4201 caaattctac cggttgtcca ctttcgacca gaattgcttt cacggtaccg gatttgtccg
     4261 cttcgatctg gttcatcatt ttcatggctt caacgatgca cagggtatcg cccacgttga
     4321 ctttctgacc cacttcgatg aacgcttttg cgtccgggct tggggtgcgg tagaaagtac
     4381 caaccatcgg ggaacgtacg atgtgaccac tgatttccgc tgctgctggc gcttccatgg
     4441 aaggaacggt cgccggagcg gctgcgttag attgagctgg ctgctgcatc attggtgcag
     4501 cgtaagcttg ttgcatcaca gggaaacttg cggcaggagc tgcacggcta atgcgtactg
     4561 actcttcgcc ttcagaaatt tccagttcgg agatgcctga ttcttcaacc agctcgatca
     4621 gttttttaat cttacgaata tccatgagtg ggttccgtac tctttgttta gtgtgattgt
     4681 gacaggcgtt tcaccgccgt ctgtaaagcg tatgccccga cagctgtatg catagcgata
     4741 aattccagca ggccgggacg ccggtctatt ttgccccaga gtgtcaatgt tagacttgac
     4801 ggacattgtg cagcgatctg ctgctatcct ccggcaaaaa acaaaatata ccttcgttgc
     4861 gccaatgtca ccttttccgc agcgcaaaaa ctgcgtcagg gaggacgagg tcgcacatta
     4921 taacgatttc gtagcaattg gcagctaaat actggtctta tcagggaaga taatcaacag
     4981 ctaacatgta aataaccttc aacaccgtgt aatttgcaac aagccgcaca attcacgaaa
     5041 ttagcgccat catcgacgga acttcttata acgtagggtg aaaagctgta aagccagccc
     5101 tgcgaagatg aggggctgca atgagataat cttcacagac cacaaataat gtagggatgc
     5161 caggatcgcg acaagataga cgacattacg cagcggttgc tgaggattac gcaattttca
     5221 ctgcatcgtc cggaaagatg tgaatgctaa agcacgcaaa attaaccgac tgacaatgtt
     5281 ccgcattcaa taggtttaag tggttaattt gcaatcaaat aaaatcagat tattcccccc
     5341 atggtaccag aaatcagtaa cagattaagc gtagcgtcgc caaggcaaaa caccataaac
     5401 ctaacaggcg acggaggcgt atcaataatg gctgtttggt ggagcatcaa tgcgcatcaa
     5461 tttatggtca gtcgaaatca tgactttcca gtttggatga tgttgtacaa cttcatgacc
     5521 aagatgttcg acgaaagctg tccggctaac cgcttttccc gttaaccgat tgaataatga
     5581 tttcaaaatg agttccctct cttattattc cctgctaaat ggttagttaa ccttcaccag
     5641 cgtgcgaccc tggatctggt tattaatgat ggcctcggca aacttcggtg cctctgccag
     5701 agatatctct tttgccgctt gggtatagaa tgattccggt aaatcggcga ccagtcgctg
     5761 ccaggcttgt gcgcggcgtg ctggtggcgt cattactgaa tccacccctt gcaaacggac
     5821 attacgcaga ataaatggca tgaccgtggt tggcagagta aaaccacccg ccagaccaca
     5881 ggccgccacg cagccgccgt aattcatttg cgccagcact tttgccagca ctttgtcgcc
     5941 aacggtgtca attgccccag cccagacttg tttttccaga ggacgggatt cagcaaactc
     6001 atcgcgagga agaatacggc tggcacctaa acttttcaga tattcatggg tactttcgcg
     6061 accggaaacg gcaacgacct gataacccaa cttatgcagc agcgtgacgg cggtactgcc
     6121 gacgccacca ctggcacccg tcacgacaac ctccccatcc tgcgggcgaa caccggcatc
     6181 ttccagcgcc atcacacaca gcatggcggt aaaaccggca gtaccgataa tcattgcttt
     6241 acgcgcgtcc agcccttgcg gcatggcaac cagccagtca cctttcactc gcgcctgttc
     6301 cgccagccca ccccagtggt tttcaccaac gccccagcca gtgagtaaca cctcctgacc
     6361 ggcatgaaaa cgcggatctt cgctggtgcg tacagttccg gcaaaatcaa tcccaggaat
     6421 catcggaaaa tttcggatga tttttccctt accggtaatc gccagcgcat ctttgtagtt
     6481 caggctcgac cagtgaacat caaccgtgac atcgccttcc ggcaggcgac tttcgtccag
     6541 agtctgtact gatgcgagag ttttgccgtc ctgctgttct aaaagtaacg cctgcataag
     6601 tggtcctcat gtgcatgatg gattggaaaa taatttctga agactatact cgctaatgga
     6661 aaagcaaagc cgatgaagcg caataaattg cgagataaat ctgatttgct agtatgcccg
     6721 cttcctcact atcggagtta acacaaggat gagattaacg acgaaatttt cggcctttgt
     6781 cacgctgctc accgggttaa caatttttgt gactttgctg ggctgttcgc taagtttcta
     6841 caacgccatt cagtataagt ttagtcatcg tgttcaggcg gtggcgacgg cgattgatac
     6901 ccacctagtg tcgaatgact tcagcacatt aaggccacaa attaccgaat taatgatgtc
     6961 ggcagatatc gttcgtgtag acctgctcca tggtgataag caggtttata ccctggccag
     7021 aaatggtagt tatcgtccgg ttggcaccaa cgatctgttt cgtgaactga gcgttccgtt
     7081 gataaagcat ccggggatgt cgctgcgtct ggtttatcag gatccgatgg gcaactattt
     7141 ccattcttta atgactaccg cgccgctcac gggggcgatt ggctttatca ttcttatgct
     7201 cttcctggcg gtacgctggt tacaacggca acttgccggg caagaattgc tggaaacccg
     7261 ggctactcgt atcttaaacg gtgagcgtgg ctctaatgtg ttgggaacca tctatgaatg
     7321 gccgcccaga accagcagtg cgctggatac gctgctttgt gaaattcaga acgcacgcga
     7381 acaacacagc cgtcttgata cgctgatccg ctcttatgcc gcccaggaca tgaaaaccgg
     7441 cctcaataac cgactctttt tcgataatca gttagcaacg ttactggaag atcaagagaa
     7501 agtaggtacc cacgggatcg tgatgatgat tcgtctgccg gatttcaata tgttgagtga
     7561 tacctggggg cacagccagg ttgaagaaca gttcttctct ctgacgaatc tgctatcgac
     7621 atttatgatg cgctaccctg gcgcactgct ggcgcgttac caccgcagtg attttgctgc
     7681 gctgttaccg caccggacgt taaaagaggc agagagcatc gccagtcagt taatcaaagc
     7741 cgtcgatacc ttgccgaaca ataaaatgct cgatcgcgac gatatgatcc acattggtat
     7801 ctgtgcctgg cgtagtggtc aggataccga gcaggtaatg gaacatgcag agtctgccac
     7861 gcgtaatgcg ggattgcagg gcggcaatag ctgggctatt tacgatgact cgttgcctga
     7921 aaaaggacgc ggtaatgttc gctggcgtac gcttatcgag caaatgctca gtcgcggcgg
     7981 cccgcgcctt tatcaaaaac cggcggttac tcgcgaaggc cgggttcatc atcgtgaact
     8041 catgtgccgc atcttcgatg gtaatgaaga ggttagctcg gcagagtata tgccgatggt
     8101 cttgcagttt ggtttatcgg aagagtatga ccgcctgcta atcagccgtc ttattccact
     8161 attgcgttac tggccagagg aaaatctggc gattcaggtt accgttgagt cgctgattcg
     8221 cccgcgtttt cagcgttggc tgcgcgatac gttaatgcaa tgtgaaaaat cgcaacgaag
     8281 acgcataatt attgaacttg cagaggccga tgttggtcaa catatcagtc gcttacaacc
     8341 tgttattcgt ttagtgaatg ctttaggggt acgggtagcc gtcaaccagg ctggtttgac
     8401 gctggtaagc accagttgga tcaaagaact taatgttgag ttactcaagc tccatccggg
     8461 gctggtcaga aacattgaga agcgaacgga gaatcagctg ctggttcaaa gcctggtgga
     8521 agcctgctcc gggaccagca cccaggttta cgccaccggc gtgcgttcgc gaagcgagtg
     8581 gcagaccctg attcagcgcg gtgttacagg tgggcaaggg gattttttcg cgtcctcaca
     8641 gccacttgat actaacgtga aaaaatattc acaaagatac tcggtttaac ctgccgttta
     8701 atccgttttc acgtagaata acgcgcgctg cgtctcatgg gagtgtgctt gtctgctcgc
     8761 cagattgttg cagcacatat gcagatgaat gaccttacgc ggttgcaaac aggcgaggaa
     8821 tgctgctgat gcattaagcc tttctggact caggcagaga tttgtaacaa aggaaacgaa
     8881 ctgcactaat tttcaccgta gcagatgatt tttgcgcctt gtcgctgctg cgtgtggttg
     8941 gtaaagtaag cggattttct tttccgcccc agctttcagg attatccctt agtatgttga
     9001 aaaaatttcg tggcatgttt tccaatgact tgtccattga cctgggtact gcgaataccc
     9061 tcatttatgt aaaaggacaa ggcatcgtat tgaatgagcc ttccgtggtg gccattcgtc
     9121 aggatcgtgc cggttcaccg aaaagcgtag ctgcagtagg tcatgacgcg aagcagatgc
     9181 tgggccgtac gccgggcaat attgctgcta ttcgcccaat gaaagacggc gttatcgccg
     9241 acttcttcgt gactgaaaaa atgctccagc acttcatcaa acaagtgcac agcaacagct
     9301 ttatgcgtcc aagcccgcgc gttctggttt gtgtgccggt tggcgcgacc caggttgaac
     9361 gccgcgcaat tcgtgaatcc gcgcagggcg ctggtgcccg tgaagtcttc ctgattgaag
     9421 aaccgatggc tgccgcaatt ggtgctggcc tgccggtttc tgaagcgacc ggttctatgg
     9481 tggttgatat cggtggtggt actactgaag ttgctgttat ctccttgaac ggcgtggttt
     9541 actcctcttc cgtgcgcatt ggtggtgacc gtttcgacga agctatcatc aactatgtac
     9601 gtcgtaatta cggttctctg atcggtgaag ccaccgcaga acgtatcaag cacgaaatcg
     9661 gttcggctta cccgggcgat gaagtacgtg aaatcgaagt tcgtggccgt aaccttgcag
     9721 aaggtgttcc acgcggcttt accctgaact ccaacgaaat cctcgaagca ctgcaggaac
     9781 cgctgaccgg tattgtgagc gcggtaatgg ttgcactgga acagtgcccg ccggaactgg
     9841 cttccgacat ctccgagcgt ggcatggtgc ttaccggtgg tggcgcactg ctgcgtaacc
     9901 ttgaccgttt gttaatggaa gaaaccggca ttccagtcgt tgttgctgaa gacccgctga
     9961 cctgtgtggc gcgcggtggc ggcaaagcgc tggaaatgat cgacatgcac ggcggcgacc
    10021 tgttcagcga agagtaatcg gatgcaggca ggggaagtgt ctgtttaccc tgcctggtct
    10081 gatacgagaa tacgcataac ttatgaagcc aatttttagc cgtggcccgt cgctacagat
    10141 tcgccttatt ctggcggtgc tggtggcgct cggcattatt attgccgaca gccgcctggg
    10201 gacgttcagt caaatccgta cttatatgga taccgccgtc agtcctttct actttgtttc
    10261 caatgctcct cgtgaattgc tggatggcgt atcgcagacg ctggcctcgc gtgaccaact
    10321 agaacttgaa aaccgggcgt tacgtcagga actgttgctg aaaaacagtg aactgctgat
    10381 gcttggacaa tacaaacagg agaacgcgcg tctgcgcgag ctactgggtt ccccgctgcg
    10441 tcaggatgag cagaaaatgg tgactcaggt tatctccacg gttaacgatc cttatagcga
    10501 tcaagttgtt atcgataaag gtagcgttaa tggcgtttat gaaggccagc cggttatcag
    10561 cgacaaaggt gttgttggtc aggtggtggc cgtcgctaaa ctgaccagcc gcgtgctgct
    10621 gatttgtgat gcgacccacg cgctgccaat ccaggtgctg cgcaacgata tccgcgtaat
    10681 tgcagccggt aacggttgta cggatgattt gcagcttgag catctgccag cgaatacgga
    10741 cattcgtgtt ggtgatgtgc tggtgacttc cggtctgggc ggtcgtttcc cggaaggcta
    10801 tccggtcgcg gttgtctctt ccgtaaaact cgatacccag cgcgcttata ctgtgattca
    10861 ggcgcgtccg actgcaggac tgcaacgttt gcgttatctg ctgctgctgt ggggcgcgga
    10921 tcgtaacggc gctaacccga tgacgccgga agaggtgcat cgtgttgcta atgaacgttt
    10981 gatgcagatg atgccgcagg tattgccttc gccagacgcg atggggccaa agttacctga
    11041 accggcaacg gggatcactc agccgactcc gcagcaaccg gcgacaggaa atgcagttac
    11101 tgcgcctgct gcgcccacac agcccgctgc taatcgctct ccacaaaggg ctacgccgcc
    11161 gcaaagtggt gctcaaccgc ctgcgcgtgc gccgggaggg caatagtggc gagctatcgt
    11221 agccagggac gctgggtaat ctggctctct ttcctcattg cgctgttgct gcaaatcatg
    11281 ccctggccgg ataacctgat tgttttccgg ccaaactggg tgttactcat cttgttgtat
    11341 tggatcctgg ccttgcctca tcgcgtaaat gtgggcacag gttttgtgat gggtgccata
    11401 ctggatctga tcagcggctc gacgcttggc gtacgcgtat tggcgatgag catcattgct
    11461 tacctggtgg cgctgaaata ccagcttttc cgcaacctcg cattatggca gcaggcgctg
    11521 gtcgtcatgt tgctttcgct ggtggtggat attattgttt tctgggcaga gtttttagtg
    11581 attaacgtct ctttcagacc ggaagtgttc tggagtagcg tagtcaatgg ggtgctctgg
    11641 ccgtggattt tcttgctgat gcgtaaagtc cgtcagcagt ttgcagtgca ataaaggttt
    11701 ctatgacttc tctgtattta gcttccggtt ctccgcgtcg tcaggagtta cttgcgcaac
    11761 ttggcgtaac ctttgaacgt attgttacgg gcattgagga gcagcgtcag ccgcaggaga
    11821 gcgcgcagca gtatgttgtg cgtctggcgc gcgagaaagc acaggcgggt gtcgcgcaaa
    11881 cggcgcagga tctcccggtg ctgggtgcgg atactatcgt tatcctgaac ggagaagtac
    11941 tggagaaacc gcgcgacgca gagcatgcgg cgcagatgtt gcgcaaatta tcgggtcaga
    12001 cccatcaggt aatgacggca gtggcgttgg ccgacagcca gcacattctc gattgcctgg
    12061 tggtcaccga tgtgactttc agaacgttaa cagacgaaga catcgcgggc tatgtcgcca
    12121 gcggtgaacc gttagataaa gcaggtgcat acggtattca ggggctgggt ggctgttttg
    12181 tcaggaagat aaatggcagc tatcacgccg tagtcggctt accgctggtt gaaacgtatg
    12241 aattattaag taattttaac gcactgcgtg agaaaaggga taaacatgac ggctgaattg
    12301 ttagtaaacg taacgccttc ggaaacgcga gtggcgtata ttgatggcgg tattctgcag
    12361 gaaattcata ttgaacgtga ggcgcgacgc ggaatagtag gcaatatcta caagggtcgt
    12421 gtaagtcgtg tacttccggg tatgcaggcg gcttttgtag atattgggct ggataaagcc
    12481 gcgtttcttc atgcatctga catcatgccg cacaccgaat gtgtggcggg tgaagaacaa
    12541 aagcaattca cggtgcgcga catctcggaa ctggttcgtc aggggcaaga tctgatggtg
    12601 caggtggtga aagatccact tggcactaag ggtgcgcgcc tgaccaccga tatcacgctg
    12661 ccttctcgct atctggtatt tatgccaggg gcttctcacg ttggggtttc ccaacgaatt
    12721 gaaagcgaat cagaacgtga acgcctgaaa aaagtggtcg cagagtattg cgacgagcag
    12781 ggcgggttta tcatccgtac cgcagctgaa ggggttggcg aggctgaact ggcctccgat
    12841 gccgcttatc tgaaacgcgt ctggaccaaa gttatggagc gtaaaaagcg cccacagacc
    12901 cgttatcagc tgtacggcga actggcgctg gcgcagcgtg ttctgcgtga tttcgccgac
    12961 gccgaactcg accgcattcg cgttgactca cgcctgactt acgaagcatt gctggagttt
    13021 acctcggagt acattcccga gatgaccagc aagctggagc attacaccgg acgccagccg
    13081 attttcgatc tctttgatgt cgaaaacgaa attcagcgag cgctggaacg caaagtagaa
    13141 ctgaaatccg gtggctatct gattatcgac cagaccgaag cgatgaccac cgtagacatc
    13201 aataccgggg cgtttgtcgg tcatcgcaat ctggacgaca ccattttcaa taccaatatt
    13261 gaagcgacgc aggctatcgc tcgccagtta cggttgcgta atctgggcgg gattatcatt
    13321 attgatttca tcgatatgaa taatgaagat caccgccgcc gcgtgctgca ctcgctggag
    13381 caggcgttga gcaaagaccg ggtgaaaacc agcgttaatg gtttttcggc gctggggctg
    13441 gtggagatga cgcgtaaacg cacccgcgaa agcattgagc acgtactatg taacgaatgc
    13501 ccaacctgcc acggtcgcgg aacggtgaaa accgtggaaa cggtatgcta tgaaatcatg
    13561 cgcgagattg ttcgtgtaca ccatgcttat gattccgacc gtttcctggt ctatgcttct
    13621 ccggcagtag ctgaagcctt gaaaggcgaa gagtcacact cgctggcgga agtggaaatt
    13681 ttcgttggta aacaggttaa agtacaaatt gaaccgctct ataaccagga gcagtttgac
    13741 gtcgtaatga tgtaaacaga tgctgggccg ccatccggca aagggttttt gagtcacatt
    13801 tttagcagac aaggagtgac gggtgaggcg attgccgggg attttactgc ttactggagc
    13861 cgcgctcgtt gtgatcgctg ccctgctggt tagcggcctg cgtattgctt taccgcatct
    13921 tgacgcctgg cgtccggaaa tcctcaacaa aatagaatcc gcgactggca tgccggtaga
    13981 agccagtcag ctctcagcca gctggcagaa ttttggtccg acgcttgaag cgcacgacat
    14041 ccgtgcagaa ctaaaagatg gcggcgaatt ttcggttaaa cgcgttactc tggcgctgga
    14101 tgtctggcag agcctgttac atatgcgctg gcagtttcgc gacctcactt tctggcagct
    14161 gcgctttcgc accaacactc ctatcaccag cggcggtggt aatgatagtc tggaagccag
    14221 tcacatcagc gatctgtttc ttcgtcagtt cgaccatttc gatctccgcg acagtgaagt
    14281 cagtttcctg acgccatccg gtcagcgcgc cgagctggcg atcccacagc tcacctggct
    14341 gaacgatcca cgtcgacacc gtgcagaagg ccaggtaagc ctctccagcc ttaccggaca
    14401 gcacggcgtg atgcaggtgc gcatggattt gcgcgatgat gaggggttgt taagcaatgg
    14461 tcgcgtctgg ctccaggcgg atgacatcga cctgaagccg tggctcggta aatggatgca
    14521 ggacaatatt gcgctggaaa cggcacagtt ctcccttgaa ggctggatga cgatcgacaa
    14581 aggcgatgta accggcggtg acgtctggct gaaacagggc ggtgccagct ggttgggcga
    14641 gaagcaaacg catacgctgt cggtggataa tctgaccgcg catattacgc gtgaaaatcc
    14701 aggctggcag ttctctattc ccgatacacg gatcacgatg gacggcaaac cctggccgag
    14761 cggagcattg acgctggcct ggataccgga acaggacgtt ggcggcaaag acaataaacg
    14821 cagtgacgaa ctccggattc gcgccagtaa tctggagctg gcaggcctgg agggcatacg
    14881 cccgctggcc gcgaaacttt cacctgcact gggtgatgtt tggcgctcca cacaaccgag
    14941 cggcaagatt aacacgctgg cgctggatat cccgcttcag gcggcagaca agacccgttt
    15001 tcaggcatcg tggagcgatc tggcctggaa gcaatggaaa ttattaccgg gtgcggaaca
    15061 cttctccggg acgctttccg gcagcgtaga aaatggtttg cttactgcgt cgatgaagca
    15121 ggcaaagatg ccttacgaaa cggtattccg tgcgccactg gaaatcgccg acggccaggc
    15181 aacgataagc tggctgaaca atgacaaagg tttccagctg gatgggcgta atattgacgt
    15241 taaagccaaa gccgtccatg cgcgcggcgg ttttcgttac ctgcaaccag ctaacgatga
    15301 accctggctg ggtattctgg ctggcatcag taccgatgat ggttcacaag cctggcgcta
    15361 tttcccggaa aacttgatgg gtaaagacct ggttgattac ttaagtggcg cgattcaggg
    15421 cggtgaagcg gataacgcga cgctggttta tggtggcaat ccgcaactct tcccctataa
    15481 acacaacgaa ggtcagtttg aagtgctggt accgctgcgc aacgcgaagt ttgccttcca
    15541 gccggactgg cctgcattaa ctaaccttga tattgaactg gactttatta acgacggttt
    15601 atggatgaaa accgatggcg ttaatctggg cggcgtgcgc gcaagtaatc tcaccgcagt
    15661 gatccctgac tactcaaaag aaaaactgct gattgacgct gacattaaag gtccgggtaa
    15721 agccgttggc ccttactttg atgagacacc gctgaaagat tctctgggtg cgaccctgca
    15781 agaactccag ctcgacggcg atgtgaatgc tcgcttacat cttgatatcc cgctgaacgg
    15841 cgaactggta accgcgaaag gtgatgtgac gctgcgtaat aacagtctgt ttatcaaacc
    15901 actcgacagc accctgaaaa atttgagcgg taaattcagc tttatcaatg gcgatctgca
    15961 aagtgaacca ctgacagcaa gctggtttaa tcagccgttg aacgtggatt tttccaccaa
    16021 agaaggggca aaagcctacc aggtcgcggt gaatctcaac ggtaactggc aaccagcgaa
    16081 aaccggcgtt ctgcctgaag cggtgaacga agcattaagt ggcagcgtgg cgtgggatgg
    16141 taaagtgggc attgatctgc cttatcatgc tggcgcgacc tataacgtag agctgaacgg
    16201 cgacctgaag aatgtgagca gtcacttacc ttcaccgtta gccaaacctg cgggtgaacc
    16261 actgccggta aacgttaagg ttgatggcaa tctcaacagc tttgagttaa ccggacaggc
    16321 tggtgcagat aaccatttca atagccgctg gttgctcggt caaaagctga cgctcgaccg
    16381 tgctatttgg gcggcagaca gtaaaacgct cccgccgttg ccggaacaaa gtggcgttga
    16441 actcaatatg ccgccgatga atggtgccga gtggctggcc ctgttccaga aaggcgctgc
    16501 ggagagtgtc ggtggtgcag cgagtttccc acaacacata acgttacgta cgcctatgtt
    16561 gtcgctggga aatcagcaat ggaataacct gagcattgtt tcgcaaccga cggcaaatgg
    16621 caccctggtt gaggcgcaag ggcgtgaaat caacgccacg ctggcgatgc gtaataacgc
    16681 gccgtggctg gcgaatatca aatatcttta ttacaaccca agcgtggcga aaactcatgg
    16741 tgactcaacg aattcatcac ctttcccgac aacggagcgc attagcttcc gtggctggcc
    16801 ggacgcacaa atacgatgcg cagagtgctg gttctggggg caaaaattcg ggcgcattga
    16861 cagtgatatc accatttctg gcaatacatt aacgctgact aatggactga ttgatactgg
    16921 tttttcgcgg ctcactgccg atggtgaatg ggttaacaat ccggggaatg aacgcacctc
    16981 gctgaaagga aaactgcgtg ggcagaaaat tgatgccgcc gcagaatttt ttggtgtcac
    17041 gacgcccata cgccagtcgt catttaatgt ggattacgat ttacactggc gcaaagcacc
    17101 ctggcaacca gatgaggcga cgttgaatgg catcattcat actcaactgg gtaaaggcga
    17161 aattaccgaa atcaataccg gacatgccgg gcaattgctg cgcttattga gcgtagatgc
    17221 cctgatgcgt aagctgcgtt ttgatttcag agacactttt ggcgaagggt tctattttga
    17281 ctccattcgc agcaccgcgt ggattaaaga cggcgttatg cacaccgacg acacgctggt
    17341 ggatggcctg gaagcggaca tcgctatgaa agggtcggta aatctggtac gtcgcgacct
    17401 gaatatggaa gcggttgtcg caccagagat ttctgcgacg gtgggtgtgg ctgcggcctt
    17461 tgcggttaac cccattgttg gcgcggcagt gtttgccgcc agtaaagtgc tgggaccgct
    17521 gtggagcaaa gtctccattt tgcgctatca catttcgggt ccgctggacg atccgcaaat
    17581 caacgaagtg ttgcgacaac cgcgtaaaga aaaagcgcaa tgatttgacg agggcgcgta
    17641 attgccccaa tctcatagga taatcgttgc caaaggccaa cgagccagaa cataaccgta
    17701 ggccggacag ggcgttcacg ccgcatccgg cagccgtaaa aaatcctcta ctgcagtaac
    17761 taacgagtag caaaaacgat gagtctgaac ctggtaagtg aacaattgct agcggcgaac
    17821 ggcctgaaac atcaggactt gttcgcgatc ctcggtcaac tggccgaacg tcgccttgat
    17881 tatggcgatc tctattttca gtcgagctat cacgaatcct gggttttaga agaccgtatt
    17941 attaaagatg gttcttacaa catcgatcag ggcgttggcg tgcgtgcaat cagtggtgaa
    18001 aaaaccggat ttgcttatgc tgaccaaatc agcctgctgg cgctggaaca gagtgcacaa
    18061 gcggcgcgca ccatcgtccg tgatagtggt gatggtaaag tacagacgct gggcgcggta
    18121 gagcatagcc cgttgtatac ctcggtagat ccgctgcaaa gcatgagccg tgaagagaag
    18181 ctggatatcc tgcgtcgcgt cgataaggtt gcccgcgaag cggacaagcg cgtacaggaa
    18241 gtgactgcca gcctcagtgg tgtctatgaa ttaattctgg ttgcggccac cgacggcacg
    18301 ctagcggcgg atgtccgtcc gctggtgcgt ctttccgtga gcgttctggt cgaagaagat
    18361 ggcaaacgcg aacgcggtgc cagtggcggc ggcggtcgtt ttggttatga gttcttcctt
    18421 gccgatctcg acggcgaagt ccgtgcggat gcatgggcaa aagaagcagt gcgtatggcg
    18481 ctggtcaatc tttctgccgt tgctgcacca gcgggcacca tgccggtagt acttggcgca
    18541 ggttggccgg gcgtgctgtt gcatgaagcg gtaggtcacg gtctggaagg cgacttcaac
    18601 cgccgtggca cttcagtatt tagtggacag gttggggagc tggtggcttc agaactgtgt
    18661 accgtggttg atgacggtac gatggtcgat cgccgtggtt cggtggcgat tgatgacgaa
    18721 ggtacgccag gccagtacaa cgtgctgatt gagaatggca tcctgaaagg ctacatgcag
    18781 gataaactca acgcgcgttt gatggggatg acgccgactg gcaacggtcg ccgtgaatcc
    18841 tacgcccatc tgcccatgcc gcgtatgacc aacacctata tgctgccggg taaatcgacc
    18901 ccgcaggaaa ttattgaatc cgttgagtac ggtatctatg caccgaactt tggtggcggt
    18961 caggtggata tcacctccgg caaattcgtt ttctccactt cagaagcata tctgattgaa
    19021 aacggtaaag taacgaagcc ggtgaaaggc gcaacgttga ttggttccgg tatcgaaacc
    19081 atgcagcaga tttcgatggt gggtaacgac ctgaaactgg ataacggcgt gggtgtctgc
    19141 ggtaaagaag ggcaaagttt gccggttggc gtgggccagc caacgttgaa agtcgataac
    19201 ctgactgttg gcggtactgc gtaataattt cagtattttc agaaggataa ttaatctttc
    19261 tacgtgcacg aacggtcccc tcgctcctct ggggttaggg tgaggggaac ccgttggcac
    19321 aggtttgtac aacgtaacag tacaatatga attacttctc tttcccgcgc ccgtgcatct
    19381 cctgaaacaa tttaccgacc tcaacaaaat aatccgtcag cgagttgatc acgacctgta
    19441 ccttcagcgg cagcttatct ttttcggtat ataacgcata aaccgggcgt ggatctgact
    19501 gataacgcgg cagcaggatc tccagttccc cacgattgat ctcgttgatc acccacatca
    19561 gcggcacgta ggcgatcccg gcacccgccg tcagccagcg caccagcgtc atcggatcat
    19621 tagtcacaaa tcttccttgt gggatcaggc gagtcgagat cccttccggt gcgatcagtt
    19681 caaattcatt gtcgggccgc acgctgtatt caagccatga atgactactc aaatcggcgg
    19741 gtttttccgg tatgccgtat tgtgtgagat agcttttcgc ggcgcacacc accatcggca
    19801 tcgcgcccag acggcgggaa aacaggctgg aatcctgcaa cgcgccgacg cggatcacca
    19861 catccagacc atcggcaatc aggtcggggg caggaattcc ggtaaccaga ttgacgctca
    19921 aacctgggta ttctttcagc atttttgctg tcagcccggc gagaacattt tgtgccatag
    19981 ttgaagaaca gccaatgcgc agcgtcccga tgggggtgtt attgaaggca tacagttgct
    20041 catgaacatc ctgcacttca tggagcatcc gacggcagcc ctggtagtaa attctgccgg
    20101 cttcagtcag gccaatgctg cgtgtactac ggtttaacag ctttacctgt aactcatctt
    20161 ccagttttga caccgtctga ctgatggacg aaacgctcat ctgtagctgt ctggcggcgg
    20221 cggtaaaaga gccaaattca actactttgg caaacaccga catgcgtttt agtcgttcca
    20281 ttattcactc tgacttaaaa gtgatttaga tcacataata tagataacag cataacagtt
    20341 acgctaatat attaaatatc aatctacagc aatgttgctc tcgcccggct ttccatgccc
    20401 ttctctgcgg cgacagatgc tgaaaataat aacgcctgct ctctctatac aaccaaggtc
    20461 aacatgagtc tgtttcccgt tatcgtggtg tttgggctgt ccttcccacc gatatttttt
    20521 gaattgcttt tatcactggc gattttctgg ctggtgcgcc gggtacttgt gccaacaggt
    20581 atctacgact ttgtctggca tccggcgttg ttcaacaccg cgctctattg ctgcttgttt
    20641 tatttgatat cgcgactgtt cgtttgaggt tgaagtgaaa acactaataa gaaaattctc
    20701 ccgtacggcc atcacggtcg tattagtcat tctggccttc atcgcaattt ttaatgcctg
    20761 ggtctattac accgaatccc cgtggacgcg tgacgcgcgc tttagcgctg acgtcgttgc
    20821 gatcgcgccg gacgtttctg gactcattac ccaggtgaat gttcatgata accagctggt
    20881 gaaaaaagga caggtactgt tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga
    20941 agcgcaagcc gatgttgctt attatcaggt actggcacag gagaaacgcc aggaggcagg
    21001 acgtcgtaac cgtctcggtg tgcaggcgat gtctcgcgaa gagatcgatc aggccaacaa
    21061 cgtactacaa acggttctgc atcagttagc gaaagcgcag gcgacccgtg atctggcaaa
    21121 actggatctt gaacgtacag tcatccgcgc gccagcagat ggctgggtga ccaacctcaa
    21181 cgtctatacc ggtgagttta ttactcgagg atcaacggcg gttgcgctgg tgaaacagaa
    21241 ctccttctat gtactggcct atatggaaga aactaagctg gaaggggtgc gccctgggta
    21301 tcgtgcggag atcacgccgc ttggcagtaa caaagtgctg aaagggactg ttgatagtgt
    21361 tgccgcaggg gtcaccaacg ccagcagcac gcgtgacgac aaagggatgg cgaccatcga
    21421 ctctaacctt gaatgggtac gtttggcgca acgtgttccg gtacgtattc gtctcgacaa
    21481 ccagcaagag aacatctggc ctgcgggcac cactgctaca gtggtggtca ctggcaaaca
    21541 agatcgcgac gaaagccagg attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt
    21601 tggttaatca cgatgggtat tttctccatt gctaatcaac atattcgctt tgcggtaaaa
    21661 ctggcgaccg ccattgtact ggcgctgttt gttggctttc acttccagct ggaaacgcca
    21721 cgctgggcgg tactgacagc ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt
    21781 gaaccgtatt ctggcgctat tcgctatcgt ggctttttgc gcatcatcgg cacatttatt
    21841 ggctgtattg ctggactggt aatcatcatt gcgatgatcc gcgcaccatt attgatgatt
    21901 ctggtgtgct gtatctgggc cggtttttgt acctggatat cctcgctggt acgaatagaa
    21961 aactcgtatg cgtgggggct ggccggttat accgcgctga tcattgtgat caccattcag
    22021 ccggaaccat tgcttacgcc gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt
    22081 attgtgtgtg cgattatggc ggatttgctc ttttctccgc gatcgatcaa acaagaagtg
    22141 gatcgagagc tggaaagttt gctggtcgcg caatatcaat taatgcaact ctgtatcaaa
    22201 catggcgatg gtgaagttgt cgataaagcc tggggcgatc tggtgcgacg caccacggcg
    22261 ttacaaggta tgcgcagcaa cctgaatatg gaatcttccc gctgggcgcg ggccaatcga
    22321 cgtttaaaag cgatcaatac gctatcgctg acgctgatta cccaatcctg cgaaacttat
    22381 cttattcaga atacgcgccc ggaattgatc actgatactt tccgcgaatt ttttgacacg
    22441 ccggtagaaa ccgcgcagga cgtccacaag cagctcaaac gcctgcggag agttatcgcc
    22501 tggaccgggg aacgggaaac gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt
    22561 tatcagcttc tcaagcgcgg cgttatcagt aacacaaaaa tcaacgctac tgaagaagag
    22621 atcctgcaag gcgaaccgga agtcaaagta gagtcagccg aacgtcatca tgcgatggtt
    22681 aacttctggc gaaccacact ttcctgcatt ctggggacgc ttttctggct gtggacgggc
    22741 tggacttccg gcagtggtgc aatggtgatg attgcggtcg tgacgtcact ggcaatgcgt
    22801 ttgccgaatc cacgcatggt ggcgatcgac tttatctatg ggacgctggc ggcgctgccg
    22861 ttagggctgc tctacttttt ggtgattatc cctaataccc aacaaagcat gttattgctg
    22921 tgtattagcc tggcagtgct gggatttttc ctcggtatag aagtacagaa acggcgactg
    22981 ggctcgatgg gggcgctggc cagcaccata aatattatcg tgctggataa cccgatgact
    23041 ttccatttca gtcagtttct cgacagcgca ttagggcaaa tcgtcggctg tgtgctcgcg
    23101 ttcaccgtta ttttgctggt gcgggataaa tcgcgcgaca ggactggacg tgtactgctt
    23161 aatcagtttg tttctgccgc tgtttccgcg atgactacca atgtggcacg tcgtaaagag
    23221 aatcacctcc cggcacttta tcagcagctg tttttgctga tgaataagtt cccaggggat
    23281 ttgccgaaat ttcgcctggc gctgacgatg attatcgcgc accagcgcct gcgtgatgcg
    23341 ccgatcccgg ttaacgagga tttatcggcg tttcaccgac aaatgcgccg cacagcagac
    23401 catgtgatat ctgcccgtag cgatgataaa cgtcgtcggt actttggcca gttgctggaa
    23461 gaacttgaaa tctaccagga aaagctacgc atctggcaag cgccaccgca ggtgacggaa
    23521 ccggttcatc ggctggcggg gatgctccat aagtatcaac atgcgttgac cgatagttaa
    23581 gtcaaaaccg acgcccgaag cgtcggtttt ttcatggcta tacttagcga tgaacggcag
    23641 aactcgccgt gaaacgtgac ggtggcaaca gatgaatatt tatacctttg attttgatga
    23701 gattgagagt caggaggatt tttatcgcga cttcagccaa gcctttggtc tggcgaaaga
    23761 taaggtacgc gatctcgact cactatggga tgtgttaatg aacgatgtcc tgccgctacc
    23821 acttgagatt gaatttgttc atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat
    23881 attgctgttt gatgaggcag aggaagagct ggaagggcat ttgcgtttta atgttcgtca
    23941 ttagcgcaaa aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt
    24001 atttgtacag ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt
    24061 aggccgttgc gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg
    24121 aagacgccac accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag
    24181 catcaatgga gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag
    24241 taacagtggt tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg
    24301 tttcgtgacc cat
//
LOCUS       DAZUCC010000059        23850 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZUCC010000059 DAZUCC010000000
VERSION     DAZUCC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.059"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(233..1144)
                     /gene="gsiD"
                     /locus_tag="ACHY09_002032"
     CDS             complement(233..1144)
                     /gene="gsiD"
                     /locus_tag="ACHY09_002032"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFS3885009.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGASIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1147..2067)
                     /gene="gsiC"
                     /locus_tag="ACHY09_002033"
     CDS             complement(1147..2067)
                     /gene="gsiC"
                     /locus_tag="ACHY09_002033"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFS3885010.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVGDEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2085..3623)
                     /gene="gsiB"
                     /locus_tag="ACHY09_002034"
     CDS             complement(2085..3623)
                     /gene="gsiB"
                     /locus_tag="ACHY09_002034"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFS3885011.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3643..5514)
                     /gene="gsiA"
                     /locus_tag="ACHY09_002035"
     CDS             complement(3643..5514)
                     /gene="gsiA"
                     /locus_tag="ACHY09_002035"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFS3885012.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYRGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVVAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5501..6466)
                     /gene="iaaA"
                     /locus_tag="ACHY09_002036"
     CDS             complement(5501..6466)
                     /gene="iaaA"
                     /locus_tag="ACHY09_002036"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS3885013.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     AMVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6670..7905
                     /gene="moeA"
                     /locus_tag="ACHY09_002037"
     CDS             6670..7905
                     /gene="moeA"
                     /locus_tag="ACHY09_002037"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFS3885014.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7905..8654
                     /gene="moeB"
                     /locus_tag="ACHY09_002038"
     CDS             7905..8654
                     /gene="moeB"
                     /locus_tag="ACHY09_002038"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFS3885015.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYHCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCGVCGQ"
     gene            complement(8730..9392)
                     /gene="fsa"
                     /locus_tag="ACHY09_002039"
     CDS             complement(8730..9392)
                     /gene="fsa"
                     /locus_tag="ACHY09_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS3885016.1"
                     /translation="MELYLDTSDVVAVKTLSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9523..10422
                     /gene="ybiY"
                     /locus_tag="ACHY09_002040"
     CDS             9523..10422
                     /gene="ybiY"
                     /locus_tag="ACHY09_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFS3885017.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10428..12860
                     /gene="ybiW"
                     /locus_tag="ACHY09_002041"
     CDS             10428..12860
                     /gene="ybiW"
                     /locus_tag="ACHY09_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFS3885018.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQAKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13006..13821
                     /gene="ybiV"
                     /locus_tag="ACHY09_002042"
     CDS             13006..13821
                     /gene="ybiV"
                     /locus_tag="ACHY09_002042"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFS3885019.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNIAPFNS"
     gene            13973..15232
                     /locus_tag="ACHY09_002043"
     CDS             13973..15232
                     /locus_tag="ACHY09_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFS3885020.1"
                     /translation="MTFSCETLPTDAKAAIREMKAALREQLSDVQQVFDTLSAKIETR
                     VAEIDALKAQGLPVWPEVSYSDIAAGTVSDATRNEIKRRGCAVIKGHFPREQAMAWDR
                     AMLDYLDINHFDDVYKGPGDSFFGSLEASRPEIYPIYWSQAQMQARQSENMAAVQSFL
                     NRLWKSESNGKQWFNPDISVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQRV
                     FANVFNGNFDDYDPWDAAHRTDVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLLHV
                     VPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAGDS
                     VWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFPRE
                     DYEANWEGRFTLEDLNIHGKRALGMDV"
     gene            complement(15473..17065)
                     /gene="ybiT"
                     /locus_tag="ACHY09_002044"
     CDS             complement(15473..17065)
                     /gene="ybiT"
                     /locus_tag="ACHY09_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFS3885021.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIIGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17284..18204
                     /gene="ldtB"
                     /locus_tag="ACHY09_002045"
     CDS             17284..18204
                     /gene="ldtB"
                     /locus_tag="ACHY09_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS3885022.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18262..19380)
                     /gene="ybiR"
                     /locus_tag="ACHY09_002046"
     CDS             complement(18262..19380)
                     /gene="ybiR"
                     /locus_tag="ACHY09_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFS3885023.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGAQTPEWKPRLVWSCLGLYIVFLMALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19377..19844)
                     /gene="mntR"
                     /locus_tag="ACHY09_002047"
     CDS             complement(19377..19844)
                     /gene="mntR"
                     /locus_tag="ACHY09_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFS3885024.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20030..20158
                     /gene="mntS"
                     /locus_tag="ACHY09_002048"
     CDS             20030..20158
                     /gene="mntS"
                     /locus_tag="ACHY09_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFS3885025.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20430..22013
                     /gene="opgE"
                     /locus_tag="ACHY09_002049"
     CDS             20430..22013
                     /gene="opgE"
                     /locus_tag="ACHY09_002049"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFS3885026.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22062..22577)
                     /gene="ompX"
                     /locus_tag="ACHY09_002050"
     CDS             complement(22062..22577)
                     /gene="ompX"
                     /locus_tag="ACHY09_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFS3885027.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22630..22695)
                     /locus_tag="ACHY09_002051"
     CDS             complement(22630..22695)
                     /locus_tag="ACHY09_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885028.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            22930..23817
                     /gene="rhtA"
                     /locus_tag="ACHY09_002052"
     CDS             22930..23817
                     /gene="rhtA"
                     /locus_tag="ACHY09_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFS3885029.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         5835 a         6469 c         5908 g         5638 t
ORIGIN      
        1 gccaataaaa ataaaattat cagccttatc tttatctttt cgtataaact cagcatgtta
       61 atacatttaa caaaataact gtctgaacgc tgtgccgctg caaaatagtc ccttgctgcg
      121 gctttttaac caaattaccc gattctacaa aatcagcaga aagttacaac acttgcttag
      181 ggcaataatt tcctgtgagg tgattaccct ttcaagcaat attcaaacgt aactatcctt
      241 taattttcgg atccagcgca tcgcgtaaac catcgcccaa caaattgaac gccagtacgg
      301 tcagaaaaat agccagggcc ggaaaaacag cgacatgcgg cgcgataacc atatccgctc
      361 gtgcctcatt gagcattgca ccccactctg gtgtcggcgg ctgcgcaccg aggccgagaa
      421 atgacaggct ggcggcagag ataatcgagg cgccaatgcg catggtgaaa aacaccacga
      481 tagaagagac ggtcccaggc aagatatgac gcaacaaaat ggtcatatcg ctggcaccaa
      541 tactgcgtgc tgactcgata aaggtttgct gtttcaacac cagcgtgttg ccgcgcacca
      601 gccgggcaaa cgcggggatg gaaaaaatgg cgactgcgat aatcacgtta gcaatgccgc
      661 ttcccaacac cgcaacaaca gcgatcgcca gtaaaatacc cgggaaggca aacaacacat
      721 cgcaaatgcg catgatcagc cgatcccacc agccttcata atatccagcc agcaagccca
      781 gcaacgtccc gatcgccgca ccgataaaca cggcaaacac gcccgccgcc agcgatattt
      841 gcgcaccaac caggacacgg ctgaaaatgt cgcgccccag tgaatcgaca ccaaaccagt
      901 gctgcaaaga aggtccgtta ttcagattgt cataatcaaa ataattttcg gcgtcatagg
      961 gagcgatcca gcgtgcaaaa atggccacca caatcaataa aataacgaac aatgcggcgg
     1021 tcatcgccat atgctgacgg cgaaatcgtc gccagaattc atgccacggt gtacgtacct
     1081 ggtcaggttt gaccagtggc atggcgttta acaccgcctg acgtcgccag ttaaatagtc
     1141 gcatccttac ttgtacctga tagccgggtt aatggcggcg taaagcacat ccaccactaa
     1201 gttgataaga ataaattcca gcgagaaaag cagaatttcc gcctgaatca ccgggtaatc
     1261 gcgcatttca acggagtcaa cgagtaagcg tccaagtccg ggccagttga aaactttctc
     1321 caccacgatg gaaccaccga gtaaaaatcc aaactgtaag cccatcatgg tcactaccgg
     1381 gatcatcgcg ttgcgtagcc cgtgtttgag gacaacccag gtttcgctca cccctttcgc
     1441 cctcgcggta cgcatataat cttcgcttaa aacgtcgaca aacgacgcgc gggtaaagcg
     1501 cgccatcacg gcggccactg ccgcgccgag cgtcagggag ggtaaaatgt agtgctgcca
     1561 actgtctgct cccacggtag gcagccagcc cagttcaaca gagaatacct gaattaaaag
     1621 catccccaga gcaaatgccg gaaacgaaat ccccgacacc gcaatagtca tactcaatcg
     1681 atccggccaa cggttacgcc agacggcggc gataattccc gccgccatac caaatatcac
     1741 cgcccagacc atactggtta tggtcagcca cagcgttggc ataaagcggc tggcaatctc
     1801 atcgccaacc ggacgacgcg acaccatcga caggccaaaa tccccctgca cagcattgct
     1861 gatatagtgc cagaactggt gatacagcgg ctgatccaac cccagctgct gacgcaccag
     1921 ttctataacc tgcgcatcag cttcgggccc ggcaattaat cgcgccggat cgccgggcag
     1981 catgtggaca aataaaaaca ccagcaccga gacgataaac agcgtcggaa tcaaccccag
     2041 taagcgtttg ataacgtaat taagcattcc actccctgcg ttgcttattg caaatccgca
     2101 tcttcaaagc tgaagccggt gtctggcatg atccaaaaac cggtcaggtt tttactgtgt
     2161 gccgacacaa gtttttctac caccagcggg atccacggcg actcttgcca gatgatatcc
     2221 tgcgccgcct tatacaagcg ggtcttttcc gccggatcat tagttttcag tgcctgagcc
     2281 aggaagtcat ccacctgttt attgctgtaa aacgcggtat taaacagcgt tggtggccag
     2341 ttctgcgagg caaacagcgg cgatagagcc cagtcggctt cgccggttga agccgaccag
     2401 ccagtgtaga acatccgcac gccgctctct ttttgccctt taccttcaac ttctgccgcc
     2461 cgctgtccgg catccatcgc agtcacctgg gctttaatcc caacctgcgc taactgctgc
     2521 tgggtaaatt gcagcacttt ctgcgcggtg ctgtggttat gtgacgacca cagcgtggta
     2581 ctgaaaccgt tgggatatcc cgcctctttc agtaattcgc gcgctttcac tggatcgtaa
     2641 ggccacggtt tataactttg cgcgtaggcg atactcggcg gtaccacacc agtagctggc
     2701 gttgcatagc ccgcgaaggc cactttcacc aacgccgggc ggttaatggc gtaattcagc
     2761 gcctcacgga ccttcgggtt atcgaacggc ttttgcgtca cgttcatact gatataacgc
     2821 tgcataattg acggactggc catcaactcg atatttttgt ttttctccag cagtgcggcc
     2881 tgctcgtagg gaatggggaa agcaaactgc gcttcaccgg tttgcagcat tgccgcgcgg
     2941 gtgttgttat ccgccaccgg acgccaggtt atgctgtcca gtttgggcaa tcctggctgc
     3001 cagtaacccg cgaatttttt caccttcaca aaatcggtct gattccaggt atccagttca
     3061 tacggcccgg ttcccaccgg atgaaaacca atctccttgc catatttttc cagcgctgcc
     3121 ggtgaaatca tcgcggtcgc cggatgggca agaatattaa taaacgctga gaatggctgt
     3181 ttgagggtaa tctttaccgt tgtcggatcg atcgcttccg ttttggcaat attcttatac
     3241 aggttatagc gtttaagatg attcgccgga tcgctggccc ggtccagatt cgcttttacc
     3301 gccgctgcgt tgaagtcggt tccatcctgg aatttaatgc cttcccgcag tttcacggtg
     3361 taagtaaggc cgtcatcgga aacggtataa ctctccgcca gcacgttttt cagtttcatc
     3421 tctttatcca gaccgaacag cccctggtaa aacgatttcg ctacggcctg agataacgtg
     3481 tcattcgcgt catatggatc gagcgtggtg aagttcgatc ctaccgccac caccacatct
     3541 ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa tgcccagcgc cactaaccca
     3601 ctacggtgta cagctcttgc cattttattc tccgcctgaa tgttatctac gcatgaatgc
     3661 gtattctgat tgtggttgtg cgacgtaatg ccccggcccg acgcattgca acgagacggc
     3721 tacaacctct tcaccacgca gatgaatatt gctgggaaga tcgtccgaca gcagtacacg
     3781 ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc gccagtaatt tacgcgtata
     3841 aggatgctgc gggttttcga agaccgcgcg ccgtggacca atttcaacaa tttgtccgcg
     3901 atacatcacc gccacacgat gactaatccg ctctaccacg gccatatcgt gggagataaa
     3961 cagatacgca atgccgaaat cacgctggag atcgagcaac aagttgataa tctgcccgcg
     4021 aatagaaaca tccagcgccg agacggcttc gtcggcaatg atcacttttg gattcaatgc
     4081 caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg gaaaactcat gagggtaacg
     4141 ccaggcatgt tccggtaaca ggcccacgcg ctccagcaac cacgcaacgc gtgcagccgc
     4201 ttctttacct ggcaataaac cgtgtacgcg cagcggttcg ataatcgaat caccgatggt
     4261 ctgacgtggg tccagcgaag cgtaagggtc ctgaaaaata aactgaatat cccggcgcaa
     4321 agcctgaagt ttaccggatg acaaggtatc gattcgctga ccgttaaaga taatttcgcc
     4381 gccctgcgat tcgaccaggc gcagcaacgc ccgcccggta gtggatttac cgctgccaga
     4441 ctcgcccacc agcgatagcg tttcgccagg ccagagatca aaactgactt tctcaacggc
     4501 atgcacttcc cgcgttacgc gattcaacaa accgctgcgc aaagggaaac gggtgaccag
     4561 attacgcacc cgtaaaacag gttcgccatc caccaccgtt ttctgctcga tggggggttc
     4621 ctgttttgct ggatgttcaa gcgatatcaa tgggaaacgt cggggataat ctaacccttt
     4681 catcgcacca agttgcggaa cagcagctaa cagcgcacgg gtataaggat gttgcggtgc
     4741 atgaaaaatc tgttcgacgc tacccgtttc caccgcctcg ccctgataca tcaccagtac
     4801 ccgatcggca atctctgcca ccacgcccat atcgtgagtg ataaagataa cgcccatcga
     4861 catctctttt tgcaatactt tgattaattg caggatctgc gcctgaatag tgacatccaa
     4921 cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg catgacagcg ccatcgcaat
     4981 catcactcgc tggcgcatcc cgccagagag ttgatgcgga taacgtgaaa ggatggtttg
     5041 tgcctccgga atgcgtacct gatccagcat ccgcttcgcc tcgaccatcg cttcttcacg
     5101 actggcgttc tgatgcagac gaattgattc ggcaatctgt tcacccacag taaataccgg
     5161 gttcagcgat gtcatcggtt cctgaaatat catcgccata tccgcaccgc gcacatggcg
     5221 catttgtgca gcgctctgct cgctaagttc aatcacttca cgactgcgcc gccgcaacag
     5281 catttgatcg cactgcacta aaccgcccgc ctgttccaac aagcgcatca atgccagcgc
     5341 agtcactgac ttaccagagc cggattcgcc aacaattgcc agcgtctcac cgcgttgcag
     5401 gctaaaagag agattgcgga ccgcagctat tttctgctgg tcctgcataa aggcaatatt
     5461 cagattttca accgccagca catcaccggc atcaagttca tcactgtgtg gcaacggtgt
     5521 cccctttttc acggtagata ccggtggttg gcgtatcgcc tgcgtagccc caggcgcgat
     5581 acattccttc ggtgttaaac ggtagcgcga cgttaccttc atggtcgata gcgattaagc
     5641 caccgctacc gccaagcgca ggaagttttt ccattactac ccgctcgcag gcttccgcga
     5701 gacttaatcc gccgtaatcc attaacgcgg cgatgtcata tgccgccagc gcgcggatga
     5761 agacttcgcc cgtgccggta caagaaaccg ccacactggc gttattggcg tagcatccgg
     5821 cacccactaa ggggctatcg ccaactcgtc cgggtaattt attggtcatt ccgcccgtgg
     5881 acgtagctgc cgccagattg ccgtctaaat ccaacgccac ggcccccacg gtgcccattt
     5941 tctgtttttc atccagtggc gcaccgctat ggtcgagaac cattgcccct tcctcgcgcg
     6001 ctgccagtag ttgttcataa cgcaaaggcg tggagaaaat ctccggcgag acgcgctcca
     6061 tgccatgagc aaacgcaaaa ttttctgccc cttcgccaat catcatcaca tgcgggcttt
     6121 gctccatcac cagccgggcg gcaagaaccg gattacgcag atgactaacg cctgccaccg
     6181 caccggcttt cagggtgtta ccatccatca cacaggcgtc cagttcatgg gtttcatcac
     6241 gcgtaaagac agcgccaatt ccggcgttaa acagtggaca ctcttccagc agacgcaccg
     6301 cttccgtcac cacatccagc gcactttcgc ccgcttccag cattttctgc ccggtttcaa
     6361 caatggcaga cagcgcctcg atgtagcgta attcctgttg cagactcatc tgcgcgcggc
     6421 taattgcacc tgcgccgcca tgaattgcaa tgactgcttt gcccatagta ttcgtccatt
     6481 aagggtggct cgccactttt gctataaata tcagaatttt tatgaattac tcattcaatt
     6541 tttgaatata gaaagatgga tatggcatgt aaaggcagga gccaacgcta cgcccgaggt
     6601 ggcatacagt ctgtggcgtt tttctgacat aataggcaaa ttcgattttg cctccgcagg
     6661 agtgttttca tggaatttac caccggattg atgtcgctcg acaccgcgct taatgagatg
     6721 ctttctcgcg ttaccccact gaccgcccag gaaacgctgc cactggttca gtgttttggt
     6781 cgtattctgg cgagcgatgt cgtttcgcca ctggatgtcc cggggtttga taactccgct
     6841 atggacggct acgcggtgcg tttagccgat attgcctccg ggcaaccgct gcccgttgcc
     6901 ggtaaatcct ttgccggtca gccgtaccat ggtgaatggc ctgcgggtac ctgcattcgt
     6961 attatgaccg gtgcgccggt gccagaaggc tgcgaagcgg tggtgatgca ggagcagact
     7021 gaacaaacgg acaatggcgt gcgttttact gctgaagtgc gtagcgggca aaatattcgc
     7081 cgtcgcggtg aagatatctc tgcaggtgcg gttgttttcc cggcggggac tcgcctgact
     7141 accgctgaac tgccagtgat tgcttcgcta gggattgccg aagttccggt gattcgtaaa
     7201 gtgcgtgtag cgcttttttc taccggtgat gaactccagt tgcccggtca gccgctgggc
     7261 gacggccaaa tctacgatac caaccgtctc gccgtacacc tgatgttaga acagttggga
     7321 tgcgaggtaa tcaacttagg gattatccgc gacgatcccc atgccctgcg cgccgcattt
     7381 attgaagccg acagccaggc ggatgtagtg atcagttccg gcggtgtttc agtgggtgaa
     7441 gcggattaca ccaaaacgat tcttgaagag ctgggggaga tcgccttctg gaaactggcg
     7501 attaaaccgg gtaaaccgtt cgcgttcggt aaactttcta atagttggtt ctgcggcctg
     7561 ccgggcaacc cggtttcagc gacgctgacc ttctatcaac tggtacagcc tttgctggca
     7621 aaactaagcg gcaataccgc cagtggcctg cccgcgcgcc agcgtgtacg cacagcgtcc
     7681 cgcctgaaaa aaacgccagg acgccttgat ttccagcgcg gcgtgctgca acgcaacgcc
     7741 gatggcgaac tggaagtgac gaccaccgga catcagggtt cacatatatt tagctccttt
     7801 agcctcggca actgctttat cgtgctggaa cgcgatcgcg gcaatgtgga agtgggcgaa
     7861 tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc tgtaatggcg gaactcagcg
     7921 atcaggagat gctgcgctac aaccggcaaa tcattctgcg cggttttgat tttgacggtc
     7981 aggaagcatt gaaagattcc cgcgtgctgg tcgtcggcct tggtgggctc ggctgcgcag
     8041 cttcgcagta tctggcaagc gccggtgtcg gcaacctgac gctgctcgac ttcgacacgg
     8101 tttcgctctc caatctgcaa cgccagacgc tgcacagtga tgccacggtc gggcaaccaa
     8161 aggtggaatc cgcccgtgac gccctgacgc ggatcaaccc acatatcgcg attacgccag
     8221 tcaatgcgct gcttgatgac gctgaacttg ctgcgatgat tgcaaaacac gatctggtgc
     8281 tcgactgtac ggataacgtt gcagtacgta atcaactgaa cgcaggctgt tttgccgcga
     8341 aggtgccgct ggtttccggc gcggcaattc gtatggaagg gcaaatcacg gtcttcactt
     8401 atcaggacgg cgaaccgtgc tatcactgcc ttagccgttt gtttggcgag aacgcgttaa
     8461 cctgcgtaga agcaggcgtg atggcaccgt taatcggcgt aattggttcg ttgcaggcaa
     8521 tggaagcaat caaactgctg gcaggttatg gcaaaccagc cagcggaaaa atcgtgatgt
     8581 acgatgcgat gacctgtcag tttcgcgaaa tgaaactgat gcgcaatccg gggtgtgggg
     8641 tgtgcgggca gtaactgcca cctccaggcc agataagacg cggcactccg ccacgtcatc
     8701 agcaatttat gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctcccagc
     8761 cagtcctgtt caaacttcgc cacagcggca tcaaccgccg gatagctaat catctgttgt
     8821 gccacatcca gcggcagagt aattgattca catcctgcca gtaagcagtc cagcgcctga
     8881 cgcggggttt tgaaactcgc ggctaacact ttcgcctgcg gcgcatgcat tttcaataac
     8941 tggtgtaagt cggtcacagt ctgaataccg ctaccgccct gagcatcaat acgattaacg
     9001 taaggcgcaa catattccgc acctgccagc gccgacagta gcccttgtgc tgcgccatat
     9061 accgcggttc ccagcgtcgg aatcccttcc gcttttaaca tcttaatagc tgccagcccc
     9121 tcagcggtca ccggaacctt caccacgata tccgcaataa tagaacgcaa tttacgtgcg
     9181 tcattaacca tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg
     9241 cccatcgcct cgtgaagttg cggaagcaca acctccagcg gttttttacc cgctgcgata
     9301 atgcttgggt tagtggtcac acccgccagc ggaaaaatac gtgacagcgt cttcaccgca
     9361 acaacgtctg aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct
     9421 gaatgtagac tatcacggga aaactctctg aacagttgac gcacatcaat ctaactttca
     9481 ttcgaaagta ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcaa
     9541 cgctactcga cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg
     9601 ctgggctgcc gctggtgtca gaacccggaa agccgcgccc gcacgcagga tctgctgtat
     9661 gacccacgac tctgtctgga aggctgtgag ctgtgcgcta agaccgcacc ggaagtgatt
     9721 gaacgcgcgt tgaatggttt gcttattcat cgggaaaagt taaccccgga gcattttacg
     9781 gcgctaaccg actgctgccc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt
     9841 gaggagatca tggcaaccgt tttgcgcgat aaaccgtttt acgatcgcag cggcggcggc
     9901 ttaacgcttt ctggcggtga accgtttatg cagccggaaa tggcgacggc gctgctgcaa
     9961 gccagccacg aggccggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa
    10021 tatatcgccc cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc
    10081 gacgcgccgt ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa
    10141 aaactcgccg cagcgggcaa aaaaatcatt atccgcgtgc cactgattca gggctttaat
    10201 gccgacgaaa cctctgtgaa agccattacc gattttgccg ccgacgagct gcacgttagc
    10261 gaaattcatt tcctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg
    10321 ccctatgacg ccccggaaaa accgcttgat gcgccagaac tgctcgactt tgcccagcag
    10381 tatgcctgcc agaaagggtt aaccgcgacc ttacgaggat aacaatcatg acaacactaa
    10441 aactggatac gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg catattgtga
    10501 aaccgccggt ctgtaccgaa cgagcacaac actataccga gatgtatcaa caacatctcg
    10561 ataagccaat cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca
    10621 tctggatcaa acatgatgaa ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc
    10681 cgatcttccc ggaatatacc gtctcctgga tcgaaaaaga gattgatgat ctggcagatc
    10741 gtcccggtgc tggttttgcg gtgagcgaag agaacaaacg cgttctgcat gaagtgtgcc
    10801 cgtggtggcg cggtcagacc gtacaggatc gctgctacgg catgtttacc gatgagcaaa
    10861 aaggtctgct ggcgaccggc atcattaaag cggagggcaa tatgacctcc ggcgatgcgc
    10921 acctggcggt caatttcccg ctactgctgg aaaaaggtct tgatggtctg cgtgagaaag
    10981 tggcggaacg tcgctcgcgc atcaatctga cggttctgga agatctgcac ggcgagcaat
    11041 tcctgaaagc gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg
    11101 ccctggcgcg tgaaatggcc gcaaccgaaa cccgcgaaag ccgtcgcgat gaactgctga
    11161 cgatagcaga aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc
    11221 tgcaactgtg ctacttcatc cagttgattt tgcagatcga atctaacggt cactcggtat
    11281 cgtttggtcg tatggaccag tatctctacc catactatcg ccgtgacgtt gaactgaacc
    11341 agacactgga tcgcgaacac gccatcgaga tgctgcatag ctgctggctg aaactgctgg
    11401 aagtgaacaa aatccgctcc ggctcacact caaaagcctc tgcgggaagt ccgctgtatc
    11461 agaacgtcac cattggcggg caaaatctgg ttgatggtca accgatggac gcggtaaatc
    11521 cactctctta tgcgatcctc gaatcttgcg gtcgcctgcg ttcgactcag cctaacctca
    11581 gcgtgcgtta ccatgcgggt atgagtaacg atttcctcga cgcctgtgtt caggtgatcc
    11641 gctgcggctt cgggatgccg gcgttcaaca acgacgaaat cgtgatcccg gaatttatta
    11701 aactcggtat tgaaccgcag gacgcttacg actacgcagc aattggttgt atcgaaaccg
    11761 ccgtcggtgg caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg
    11821 tgatgctggc ggcgctggaa ggtggtcgtg atgccaccag cggcaaagtg ttcctgccac
    11881 aagcaaaagc gttgtcagcg ggtaacttca acaacttcga tgaagtaatg gacgcatggg
    11941 atacgcaaat ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgatacca
    12001 tgctggaaga gaacgtacac gatattctct gctcggcgct ggtcgatgac tgtattgagc
    12061 gagcgaaaag tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg
    12121 gcattgctaa cctcggcaac agcctggcgg cagtgaagaa actggtattt gaacagggcg
    12181 cgattggtca gcaacagctg gctgccgcac tggcggatga cttcgacggc ctgactcacg
    12241 agcagctgcg tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgatactg
    12301 tcgatacgct gctggctcgc gcttatcaga cctatatcga cgaactgaag cagtaccata
    12361 acccgcgcta cggtcgtggt ccgattggcg gcaactatta tgcgggcaca tcgtctatct
    12421 ccgctaacgt accgtttggc gcgcagacga tggcaacgcc ggacggacgt aaagcacata
    12481 ccccgctggc agaaggcgct agtccggcct ccggtactga ccatctcggc cctactgcgg
    12541 tcattggctc ggtgggtaaa ctgcctacgg cagcaattct cggcggcgtg ctgctcaacc
    12601 agaaactgaa tccggcaacg ctggagaatg aatctgacaa gcagaaactg atgatcctgc
    12661 tgcgtacctt ctttgaagtg cataaaggct ggcatattca gtacaacatt gtttcccgcg
    12721 aaacattgct ggaggcgaag aaacatccgg atcaataccg cgatctggtg gtacgcgtag
    12781 cgggttattc cgccttcttc accgcgctct ctccagacgc acaggacgat atcatcgccc
    12841 gtaccgaaca catgctgtaa tcccctcagc ccggcgtcgt cgtcgccggg ccaaatcatt
    12901 cacatcatca atttcagcct cgctttcatt cgaaatataa tttgtgctct gcgtcacatt
    12961 gttattacac tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt
    13021 atcgtcacag acatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt
    13081 tttatggcgc aatatcagga actgaaaaag cgcggcattg agttcgttgt cgccagcggt
    13141 aatcagtatt accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc
    13201 gcggaaaacg gcgcactggt ttacgaacat ggcaagcagt tgttccacgg cgaactgacc
    13261 cgacatgaat cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc
    13321 gcctgcggtc tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt cgtcgcactg
    13381 atggcaaaac actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta
    13441 ctgttcaagt tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaactg
    13501 cacatagcgc tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacttg
    13561 attattcccg gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg
    13621 tcaccgcaaa atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg
    13681 gcgcgttatt cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac
    13741 gctaccgatg ataataatca tgaaggcgcg ctgaatgtga ttcaggcggt gctggataat
    13801 atcgctcctt tcaatagttg atcttccccc tggccgaagt gaccacttcg gcctctctcc
    13861 tttttgctcg ctttgtgcac tgactcaata tttttatctt gcgttaactt atttttaact
    13921 taatgcatca atcgcaatca ttttactttc gaatgaaaga agcgaggttg atatgacttt
    13981 ttcctgtgag acgctgccca cagatgcgaa agcagctatc cgagagatga aagctgcgct
    14041 gcgggaacag ctcagtgatg tgcaacaggt tttcgataca ctctctgcaa aaatcgaaac
    14101 gcgtgttgct gaaatcgatg ccctaaaagc gcaagggctg ccggtttggc cggaagtttc
    14161 ttatagtgat attgccgcag gtacagtaag cgatgccact cgcaatgaaa ttaaacgtcg
    14221 cggttgtgca gtcataaaag gccatttccc tcgcgagcag gcgatggcgt gggatcgtgc
    14281 catgctcgat tatcttgata tcaatcattt tgatgatgtt tataaaggcc ctggcgacag
    14341 ttttttcggc tcgctggagg cttctcgtcc cgaaatctat cccatttact ggtcgcaagc
    14401 acagatgcag gcgcggcaga gcgaaaacat ggctgctgtg caatcttttc taaaccgact
    14461 gtggaagtct gaaagcaacg gcaaacagtg gtttaatcct gatatcagtg tgatttatcc
    14521 agacaggatc cgccgccgcc ctccaggtac tacatctaaa ggcctgggcg cgcataccga
    14581 ttctggtgcg ctggaacggt ggttattacc cgcttatcag cgcgtttttg ccaatgtgtt
    14641 taatggtaat ttcgatgatt acgatccctg ggatgctgca caccggaccg atgtcgaaga
    14701 gtacacggta gataacacta caaaatgttc ggtgttccgc acgtttcagg gctggacggc
    14761 gctctctgat atgttatcgg gtcagggatt gttacatgtg gtgcctatcc cggaagcgat
    14821 ggcgtatgtt ttattgcgcc cgctgctgga tgacgtaccg gacgatgaac tgtgcggggt
    14881 tgcacctggc aaagttctgc ccatttcaga gaaatggcat ccactactgc ttaaagcgct
    14941 gacttccatt cccgcgataa cagcagggga ttccgtctgg tggcactgcg atgtgattca
    15001 ctccgtcgcg ccagtagaca atcaacaggg ttggggcaac gtgatgtata tccctgctgc
    15061 gccaatgtgc gagaaaaatc gggcttatgc acacaaagtc aaaatggcgc tggaaaaagg
    15121 cgcatcacct ggcgacttcc cgcgggaaga ttatgaagca aactgggaag gccgctttac
    15181 gctggaggat ctgaacattc acggtaagcg cgcactgggc atggatgttt gagagttaat
    15241 ggaagtgaat tgatgtggga ttaagaaaat cgattcaacg ttactcagat tgctgacaaa
    15301 tgcgctttgt taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag
    15361 attcaatata ttgcaggtac tgcgtaggcc tgataagcat agcgcatcag gcaatcttgc
    15421 gtttgtcatc agccgccgtc accccgacag tatcgggctg acggataata acttactcga
    15481 tccctttact acgcagataa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg
    15541 tgatttccag aattcgggtc gccagggagc ttacaaactc acggtcgtga gacacaaaga
    15601 tcagcgtgcc ctgatacagt tccagcgcca tgttcagcga ctcaatggat tccatatcca
    15661 ggtggttggt cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa
    15721 acagcatccg ccctttctcc ccaccggaaa gcactttcgc tggctttttg atgtcgtcct
    15781 ggctgaacag caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct
    15841 tccactgact catccattcg aacacggtaa gatcattttc gaactcatat tcgtggtcct
    15901 gggcgtagta accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca
    15961 gatcgcccac cagcgttttc aacagtgttg atttaccgac gccgttggta cccagtaccg
    16021 ccagtttttc accgacttcc agcagcaggt tgagattttt aaacagcgga ccgttatcaa
    16081 accctttggt cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa
    16141 aacggatgaa cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa
    16201 tctggcgcgc gcgcgaagtt gcctggcgag atttcgaggc gttggcacta aagcggctaa
    16261 cgaaagattg cagctcagca atctgcgctt tcttcttggc gttatcagcc agcagacgct
    16321 cacgcgcctg ggtcgccgcc gtcatgtact catcgtagtt acctggataa acgcgcagct
    16381 cgccgtaatc cagatccgcc atgtgggtac aaaccatgtt aaggaagtga cggtcgtgcg
    16441 agatgatgat catggtgctg tcacgctcgt tcagcacctg ttccagccaa cgaatggtgt
    16501 cgatgtcgag gttgttggtc ggttcgtcga gcaggagaat gtccggatca gcaaacagcg
    16561 cctgcgccag cagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt
    16621 gctgttccac tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt
    16681 aaccgtccat ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac
    16741 tcatttccgg caaagcatag atgcggtcac gctcctgctt cacttcccac aactctttat
    16801 gcccaatgat caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca
    16861 gcttaccaat gcgctcgttg ggatcgaggg aaacgttacc caacgtcggc tctaagtcgc
    16921 cgccgaggat cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac
    16981 ggttgccgcc gccaaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca
    17041 tggtgacgtt actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc
    17101 attatgccat aaatctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa
    17161 aggcaaaaaa tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa
    17221 tgcgcttcca atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt
    17281 aacatgaata tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc
    17341 agtaccgcct ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag
    17401 aatcaggtga tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgccgag
    17461 taccagatgg gactttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg
    17521 aaaggcggta ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa
    17581 ggcatcgtca ttaacagtgc agagatgcgt ctgtattact atccgaaagg aaccaacacc
    17641 gttatcgtgc tgccgatcgg cattggtcag ttaggcaaag acacgcctat caactggacc
    17701 accaaagttg agcgtaaaaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca
    17761 gagtaccgcg ctgcgggcga accgcttccg gctgtcgttc cggcaggtcc ggataacccg
    17821 atgggtctgt atgcactcta catcggtcgc ctgtacgcta tccatggcac caacgccaac
    17881 ttcggtatcg gcctgcgtgt aagtcatggc tgtgtgcgtc tgcgtaacga agacattaaa
    17941 ttcctgttcg agaaagtgcc tgtcggtact cgcgtgcagt ttattgatga gccggtgaaa
    18001 gcgactaccg aaccagacgg cagccgttac attgaagtcc ataacccgct gtctaccacc
    18061 gaagcccagt ttgaaggtca ggaaattgtg ccaattaccc tgacgaagag cgtgcagaca
    18121 gtgaccggtc agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc
    18181 gggatgccgg ttcgtctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc
    18241 gcctttttta tttgccagac cctaccaggc cgggatcata accaacaaaa catatcccac
    18301 cagcgccgcc cacaacagca tcggtatcga ataaagatga aaacgccacc agatacggcg
    18361 atcgttcgcc atccgtaacg caatcaaatt tgccagcgat ccgggtaata aaccaaagcc
    18421 ccctacgttt accgcccatg ccagcagcaa cgacggcggc acatagttca gcaacaatat
    18481 ggtactcggc acgttactga tcacctgcga taaaccgatt gccgttaacc ataatccggg
    18541 ttcggataaa tgactcacgt tacttaacac cccctgcaaa gctggcagtt gggttagtaa
    18601 atggacgtcg ataaacatcg ccataaacac cagcagcagc gtccagtcca cactgagcac
    18661 cacgcgacgt gccagcagcg caaagcccac cgccacaatc accagtcccc acagctcttg
    18721 ttttaactcc agcgccatca gaaagacgat atacagcccc agacaactcc acaccagccg
    18781 cggtttccac tccggcgttt gcgcccccgt atggtattgc agcgcctttc cagggaaaca
    18841 acaccagcac aacagcagga gtgtcagcat cattgcgcca gccagcggtg ccatttgggc
    18901 aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc
    18961 aattggcgtc agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt
    19021 aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc
    19081 gacatcgttg gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag
    19141 ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct
    19201 taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc
    19261 gatagcagca ggccaggatt tcggtgcaaa gggaacgaaa aaacttaatc cgatgccaac
    19321 aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat
    19381 ttggcaccgt gtttctgggt aaacaaacga aaggcatcca gcgtctcttc actaacatgg
    19441 tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag
    19501 aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagtttctc tccttctgcc
    19561 gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagtcgctta
    19621 agcattttag ccaccgtcgg ttgcgaaact cccaaacgag cagccatgtc tacctgacga
    19681 gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc aatcagctcg
    19741 cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac
    19801 tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct tcctcattat
    19861 ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt
    19921 acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta
    19981 atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt
    20041 caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc
    20101 tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg
    20161 gcgtcgcggt acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa
    20221 aaaccccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt
    20281 gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat
    20341 gcggacccct ttcccctctc cacgcactct tgcaggtatt gacccttgac gccaaggtaa
    20401 gcacatggcg tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta
    20461 cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta
    20521 acctcggctt aggttatccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc
    20581 ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg
    20641 tggcggcttg ttatttccct tttgctcagg cctacggcgc acctaatttc aatacattgc
    20701 tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt
    20761 acagctacct ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcggaaaa
    20821 aagagagtga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg
    20881 cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag
    20941 atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga
    21001 ttgaagagca agaacggatg gcaaaactgt ccggaatgaa agatacctgg acggtcactg
    21061 ccgttaagcc gaagtatcag acctatgtgg tggtgatcgg agaaagcgcg cgtcgcgatg
    21121 ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt
    21181 tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt ggcttaacgc
    21241 tcaatcgcgt tgtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc
    21301 gcgcgggctt ccagacctgg tggttttcca accagggcca aatcggcgaa tacgataccg
    21361 ccatcgccag catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg
    21421 aagcagataa aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc
    21481 aagagcactc gcaaccgcag ctgattgttt tacatctgat gggctcacat ccgcaggcct
    21541 gcgaccggac acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc
    21601 tctataccat gacgcaaaca gacgatttac tgcgtaagct gtacgatcag ttacgcaaca
    21661 gcggcagtag cttctcgctg gtttactttt ctgaccacgg tctggccttt aaagagcgtg
    21721 gcaaagacgt gcaatacctt gcccatgatg ataagtatca gcaaaatttc caggtgcctt
    21781 ttatggtcat ttcgagcgac gataaagcgc atcgggtgat taaagcccgc cgctctgcca
    21841 atgacttctt aggctttttc tcccagtgga cgggaattaa agcgaaggaa ataaatatca
    21901 aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac
    21961 agaaggtaga ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa
    22021 aatccgcccc gagaggcgga ttttttatat caccgaagtg attagaagcg gtaaccaaca
    22081 ccggcaatcc aggtgcctac gtcaacgcta cgaatacggc tctgctcgta agagaagtcc
    22141 agagcaacgt tttccatcgg gttgaactgc agaccagcac cgtaggagaa accgtagtcg
    22201 ctggtgtcgt gtttgtaggt cgggtattca gtggtctgga atttaccata acccacaccc
    22261 actacaccgt agatgcttgc ccagtcgtta atgcggtaag ccggaccagc agtgatgccg
    22321 tagtactggt ttttgttgta gtcaccagag cttgcagtac ggcttttctc ggtgtaagtg
    22381 aaagaaccga tcacacccag cgggctgttg tcttcttcat agcggtattt caggttgaaa
    22441 ccgcccattt tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc agttacggta
    22501 gaagtcgcag ctacggaagt acctgcggtg aaagccagaa ctgcggccag tgctgaaaga
    22561 catgcaattt ttttcataac cacctcaaat gtgattcaaa taagtcctaa gttttaaata
    22621 tatcaaaaat taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc atttcatgta
    22681 acagaacgtt tccatacacc gctatccatc taaatttaaa tcactttttc agagagctgc
    22741 ataagtatta cgcatgtttt ccctgtcatt catccagatt attcctaatc accagactaa
    22801 tgattccatc aatcctggcg cattttagtc aaaacggggg aaaatttttt caacaaatgc
    22861 tcaaccagca ttgggtatat ccagtacact ccacgcttta cttaggttta gatatttgtg
    22921 ggagaaagga tgcctggttc attacgtaaa atgccggtct ggttaccaat agtcatattg
    22981 ctcgttgcca tggcgtctat tcagggtgga gcctcgttag ctaagtcact ttttcctctg
    23041 gtgggcgcac cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat cctcatcgcg
    23101 ttctttaagc catggcgact gcgctttgcc aaagagcagc ggttaccgct gttgttttac
    23161 ggcgtttcgc tgggggggat gaattatctt ttttatcttt ctattcagac ggtaccgctg
    23221 ggtattgcgg tggcgctgga gttcaccgga ccactggcgg tggcgctgtt ctcttctcgt
    23281 cgcccggtag atttcgtctg ggttgttctg gcggttcttg gtctgtggtt cctgctaccg
    23341 ctggggcaag acgtttccca tgtcgattta accggctgtg cgctggcact gggagcaggg
    23401 gcttgttggg ctatttacat tttaagtggg caacgcgcag gagcggaaca tggccctgcg
    23461 acggtggcaa ttggttcgtt gattgcagcg ttaattttcg tgccaattgg agcgcttcag
    23521 gctggtgaag cactctggca ctggtcggtt attccattgg gtctggcggt cgctattctc
    23581 tcgaccgctc tgccttattc gctggaaatg attgccctca cccgtttgcc aacgcggaca
    23641 tttggtacgc tgatgagcat ggaaccggca ctggctgccg tttccgggat gattttcctc
    23701 ggagaaacac tgacacccat acagctgctg gcgctcggcg caattatcgc cgcttcaatg
    23761 ggttctacgc tgacagtacg caaagagagc aaaataaaag aattagacat taattaaatt
    23821 tacatttctg catggttatg cataaccatg
//
LOCUS       DAZUCC010000060        23518 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZUCC010000060 DAZUCC010000000
VERSION     DAZUCC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.060"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1375
                     /gene="cdgI"
                     /locus_tag="ACHY09_002053"
     CDS             <1..1375
                     /gene="cdgI"
                     /locus_tag="ACHY09_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFS3885030.1"
                     /translation="KSSTHIALLFMASMGTQKLKAQGFFIFSLLLTLILFCITTLYNE
                     NTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLV
                     ETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKT
                     GILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFL
                     LFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVV
                     SFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQF
                     KRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAE
                     RMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADQALREAKSEGGN
                     KVIIHHI"
     gene            1556..3046
                     /gene="dgcJ"
                     /locus_tag="ACHY09_002054"
     CDS             1556..3046
                     /gene="dgcJ"
                     /locus_tag="ACHY09_002054"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFS3885031.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPDLRGTLQTASTDCDTIVEAAALLPAFDQAVESNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3089..3592
                     /gene="yeaK"
                     /locus_tag="ACHY09_002055"
     CDS             3089..3592
                     /gene="yeaK"
                     /locus_tag="ACHY09_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFS3885032.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3593..3697)
                     /gene="yoaI"
                     /locus_tag="ACHY09_002056"
     CDS             complement(3593..3697)
                     /gene="yoaI"
                     /locus_tag="ACHY09_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFS3885033.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3867..4313
                     /gene="yeaL"
                     /locus_tag="ACHY09_002057"
     CDS             3867..4313
                     /gene="yeaL"
                     /locus_tag="ACHY09_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS3885034.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4270..5091)
                     /gene="nimR"
                     /locus_tag="ACHY09_002058"
     CDS             complement(4270..5091)
                     /gene="nimR"
                     /locus_tag="ACHY09_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFS3885035.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGVSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5188..6369
                     /gene="nimT"
                     /locus_tag="ACHY09_002059"
     CDS             5188..6369
                     /gene="nimT"
                     /locus_tag="ACHY09_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFS3885036.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSTMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVAAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6424..6771
                     /gene="yeaO"
                     /locus_tag="ACHY09_002060"
     CDS             6424..6771
                     /gene="yeaO"
                     /locus_tag="ACHY09_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS3885037.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLANIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6793..7047)
                     /gene="yoaF"
                     /locus_tag="ACHY09_002061"
     CDS             complement(6793..7047)
                     /gene="yoaF"
                     /locus_tag="ACHY09_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFS3885038.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7230..8255
                     /gene="dgcP"
                     /locus_tag="ACHY09_002062"
     CDS             7230..8255
                     /gene="dgcP"
                     /locus_tag="ACHY09_002062"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFS3885039.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8288..8386
                     /gene="yoaK"
                     /locus_tag="ACHY09_002063"
     CDS             8288..8386
                     /gene="yoaK"
                     /locus_tag="ACHY09_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFS3885040.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8389..8463
                     /gene="yoaJ"
                     /locus_tag="ACHY09_002064"
     CDS             8389..8463
                     /gene="yoaJ"
                     /locus_tag="ACHY09_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFS3885041.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8522..8770)
                     /gene="yeaQ"
                     /locus_tag="ACHY09_002065"
     CDS             complement(8522..8770)
                     /gene="yeaQ"
                     /locus_tag="ACHY09_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS3885042.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8918..9100)
                     /gene="yoaG"
                     /locus_tag="ACHY09_002066"
     CDS             complement(8918..9100)
                     /gene="yoaG"
                     /locus_tag="ACHY09_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFS3885043.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(9104..9463)
                     /gene="yeaR"
                     /locus_tag="ACHY09_002067"
     CDS             complement(9104..9463)
                     /gene="yeaR"
                     /locus_tag="ACHY09_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFS3885044.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9636..10274)
                     /gene="leuE"
                     /locus_tag="ACHY09_002068"
     CDS             complement(9636..10274)
                     /gene="leuE"
                     /locus_tag="ACHY09_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFS3885045.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10401..11324)
                     /gene="dmlR"
                     /locus_tag="ACHY09_002069"
     CDS             complement(10401..11324)
                     /gene="dmlR"
                     /locus_tag="ACHY09_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFS3885046.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11427..12512
                     /gene="dmlA"
                     /locus_tag="ACHY09_002070"
     CDS             11427..12512
                     /gene="dmlA"
                     /locus_tag="ACHY09_002070"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFS3885047.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKKIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVID
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            12763..14373
                     /gene="yeaV"
                     /locus_tag="ACHY09_002071"
     CDS             12763..14373
                     /gene="yeaV"
                     /locus_tag="ACHY09_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFS3885048.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHHV
                     EHYLPQTPVEVLEKTPVLPAGTVFKGDN"
     gene            14405..15529
                     /gene="yeaW"
                     /locus_tag="ACHY09_002072"
     CDS             14405..15529
                     /gene="yeaW"
                     /locus_tag="ACHY09_002072"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFS3885049.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15585..16550
                     /gene="yeaX"
                     /locus_tag="ACHY09_002073"
     CDS             15585..16550
                     /gene="yeaX"
                     /locus_tag="ACHY09_002073"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFS3885050.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAELQYSDVDWQLHYCTRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLNIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16597..17712)
                     /gene="rnd"
                     /locus_tag="ACHY09_002074"
     CDS             complement(16597..17712)
                     /gene="rnd"
                     /locus_tag="ACHY09_002074"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFS3885051.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17794..19479)
                     /gene="fadD"
                     /locus_tag="ACHY09_002075"
     CDS             complement(17794..19479)
                     /gene="fadD"
                     /locus_tag="ACHY09_002075"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFS3885052.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDSEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19684..20265)
                     /gene="yeaY"
                     /locus_tag="ACHY09_002076"
     CDS             complement(19684..20265)
                     /gene="yeaY"
                     /locus_tag="ACHY09_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFS3885053.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(20304..20999)
                     /gene="tsaB"
                     /locus_tag="ACHY09_002077"
     CDS             complement(20304..20999)
                     /gene="tsaB"
                     /locus_tag="ACHY09_002077"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS3885054.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGADLPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDEKGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLTLCDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(21057..22967)
                     /gene="yoaA"
                     /locus_tag="ACHY09_002078"
     CDS             complement(21057..22967)
                     /gene="yoaA"
                     /locus_tag="ACHY09_002078"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS3885055.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            23099..23443
                     /gene="yoaB"
                     /locus_tag="ACHY09_002079"
     CDS             23099..23443
                     /gene="yoaB"
                     /locus_tag="ACHY09_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFS3885056.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5785 a         5983 c         5698 g         6052 t
ORIGIN      
        1 aaaatccagc actcacattg ctctcctttt tatggcttct atgggtacac aaaaattaaa
       61 agctcaaggc ttttttattt tcagcttatt gctgacgtta attttatttt gcattactac
      121 cttatataac gaaaacacga atgtaaaact catcccacag atgaattacc tgatggttgt
      181 tgtggctttg tttttcctta acgccgtcat tttccttttc atgttaatga agtatttcac
      241 taacaaacaa attttaccaa cactcatttt aagccttgca tttttaagtg gccttatcta
      301 tttagttgaa accattgtaa ttatccataa accaatcaac ggcagtacac tgatccagac
      361 aaagtcgaat gatgtttcta ttttctatat tttccgccaa ctcagtttca tttgtttaac
      421 ctcgctggcg ctcttttgtt atgggaaaga caacatcctt gacaacaata agaagaaaac
      481 tggcattctg ttgctggcgc tgattccttt tttagttttt ccccttctgg cacacaatct
      541 gagcagttat aacgctgact attctttgta tgtcgtcgat tactgtccgg acaaccatac
      601 tgcgacctgg ggaatcaact atacaaaaat attggtttgt ttgtgggcat ttttactgtt
      661 ctttattatc atgcgtacac gattagccag cgaactatgg ccgttaatag cattattatg
      721 tctggcatcg ctatgctgca acttacttct actgactctg gatgagtata attacaccat
      781 ctggtatatc agtcgcggga ttgaagtttc cagtaaactg tttgttgtgt cttttctgat
      841 ttataacatt tttcaggaac tgcaactctc cagcaaactg gcagttcatg atgtactgac
      901 caatatttat aatcggcgct actttttcaa cagcgtagag tcattattgt cgcgacctgt
      961 tgttaaggac ttttgtgtca tgctggttga tattaatcag ttcaaacgca tcaatgccca
     1021 atggggacat cgtgtgggtg ataaagtgct ggtatcaatt gtcgatatta tccagcaaag
     1081 catccgcccc gatgatattt tagcgcgact ggagggtgag gtgtttggct tgctatttac
     1141 cgaactcaat agtgcccagg caaaaatcat tgcggaacgt atgcgtaaaa atgtcgaact
     1201 cctgaccggc tttagtaaca gatatgatgt tcctgagcaa atgaccatca gtattggcac
     1261 ggttttttca acgggtgaca cacgtaatat ctcgcttgtc atgacggaag cagatcaagc
     1321 cttacgcgaa gcgaaaagcg aggggggcaa caaagtgatt attcatcata tttaagtgca
     1381 aaaatattca gagccatgct ttttgcgtgg cttttgcata caatttatta ctattacccc
     1441 taaatttccc ctccccagcg gcgcagagat gagtataatt agcgcccctg tgccaggccg
     1501 caatcgaact ttatctggtt ttctcgtttc actaaccgaa ggagtgccat ttatcatgaa
     1561 attgcaccat agaatgctcc ggcattttat cgccgcaagt gtcattgtgc tgacatcttc
     1621 cttccttatt tttgaacttg tcgccagcga cagagcaatg agtgcctatc tgcgctatat
     1681 cgtgcagaga gcagactcct cctttcttta tgataagtat cagaatcaga gtattgccgc
     1741 gcatgtgatg cgcgctctcg ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc
     1801 catctgcgag gcttttgagt ctgccaataa tacccatggc ttaaacctga ctgcccataa
     1861 atacccggac ttacgcggca cactacaaac cgcatccact gactgcgaca caattgtgga
     1921 agctgcagca ctattacccg cttttgatca ggcagtggaa agcaaccgcc accaggatga
     1981 ttacggttca ggtcttggga tggccgaaga gaaatttcac tattatctcg atctcaatga
     2041 ccgctatgtc tatttttatg aaccggttaa tgttgagtac tttgcgatga ataactggtc
     2101 tttcctgcag tcaggaagta ttggcatcga tcgcaaagat attgaaaagg tatttaccgg
     2161 gcgtaccgtt ttatcgagca tttaccagga tcagcgtact aaacagaacg tgatgagttt
     2221 gctgacgccg gtatatgtcg cagggcagct aaaagggatt gtgctgctgg atattaacaa
     2281 aaacaatctg cggaatatct tttacactca tgaccgccct ctcctctggc gttttctcaa
     2341 tgtcacgcta accgataccg attcggggcg cgacattatc atcaaccaga gcgaagataa
     2401 tctgttccag tatgtcagtt acgtccatga cttaccgggc ggcattcgtg tctcgttatc
     2461 cattgatatt ctttacttta tcacgtcttc gtggaaaagc gttctgttct ggattttgac
     2521 ggcgttaatt ttgctgaata tggtgcggat gcacttccgt ttataccaaa acgtgtcgcg
     2581 agaaaatatt agtgatgcga tgactggact gtataatcgc aaaattttaa cccctgaact
     2641 ggagcagcgg ttgcagaagc tggtgcaatc cggttcttcg gtgatgttta ttgcaattga
     2701 catggacaag ttaaagcaaa taaatgacac cctcggtcat caggaggggg atttagcgat
     2761 tacgttatta gctcaggcga ttaaacaatc gattcgtaaa agtgattatg ccatccgact
     2821 cggtggcgat gaattctgca tcattcttgt cgattcgacg ccgcaaattg cagcacaact
     2881 gcctgaacgt atcgaaaaac gtctgcaaca tatcgcgccg cagaaagaga tcggcttctc
     2941 ttccggtatt tacgcgatga aagaaaacga tacgttacat gatgcgtata aagcttccga
     3001 tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac cttctgtggt
     3061 tgtttgcttg taatctcagg agcatgaaat gactgaaatg gctaaaggaa gcgtgacgca
     3121 tcagcggtta atcgctttat tatcacaaga aggtgctgac ttccgcgtcg ttacccatga
     3181 agcggtaggt aaatgtgaag cggtgtcaga aattcgtggc accgctttag gccagggggc
     3241 taaggcactg gtatgtaaag tcaaaggcaa tggtgtaaat caacatgttc tggcgatcct
     3301 cgcggccgat caacaggccg atctgagcca actcgccagc catataggcg gattacgcgc
     3361 ttcgttggcc agcccggcgg aagtcgatga actcacaggc tgtgtcttcg gcgcgatccc
     3421 ccctttcagc ttccatccaa aactcaaact ggttgccgat cccctactct tcgaacgatt
     3481 tgatgaaatc gccttcaatg caggcatgct ggataaatcc gttattttga aaaccgcgga
     3541 ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt aactagcctg
     3601 tccgttcgat aagaagaacg gacaaaacca gcacaacagc aatggcaaaa aacgatgacg
     3661 tgataatcag tgtctcgacg aacatttgat cgttcatagt atgcccccgg gagatagttg
     3721 ttttcacgtt attgttagcg tgcgcaaatg gcagtttgat gacagttcgc ctgatttttt
     3781 atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcgtaaag taagcaggct
     3841 tattattctt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc ctgctcgggt
     3901 tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt ctggtgttaa
     3961 tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa caggggctaa
     4021 gtatcgggat aatcattctg acgattggcg ttatggctcc catcgccagc gggacgctac
     4081 cgccctcaac cctgattcac tcgtttctga actggaaatc gctggtggca attgcagtcg
     4141 gcgttattgt ctcctggctg ggggggcgcg gcgtgacgtt aatgggcagc cagccgcaac
     4201 tggtcgccgg gctgctggtg ggcactgttt tgggcgtggc gctgtttcgc ggcgtaccgg
     4261 ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag tagttaatcc
     4321 cgcaatatat cggcctggcg tttgccccag gcctttctta aacatagtaa taaaggccgt
     4381 tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt tcactaatcc
     4441 ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca cccccgtctc
     4501 tttcactatc agtcgggcaa gattgcgttc actcatcgca aaaaagcgag cccattgccc
     4561 caatgacccc cactcgacgg gctctttcgc catcatctcc accatcgcgc ggattttagg
     4621 atgagaagaa acgggtaggt gcagctgttg ttgtggctgc tgagggagtt catcaaacag
     4681 gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc tgtttgccag
     4741 ggttaaaatt aattcccggc ataagggcga tattttcaat gtgcaacagg ttgtcggcat
     4801 tgtcactgcg ctgggttcga taaacagaaa acagagttca gcgttagctg tcacttgatt
     4861 gctatgttcc acccctcccg gtatccaaac cgcatattgt ggcggcacca tccacaaagc
     4921 attttccacc gtacaggtaa ttgcaccatg tagtgccagt atcaactgtc ctttgcggtg
     4981 ctgatgtaca ggactggtta actcatccgt tccggcgtga atacaaaatg cgaccgcggc
     5041 ttcatggtgt cggtcgggtt catagccatt gagattcaac ctgtgcatca ttttgtccga
     5101 atttagcgat aatttgtcat tttagcttga ttcaacataa caataaaaac ggtaaggtac
     5161 agcctcgttt gtaacaatga gaagcatatg acctgttcaa cttcattaag cggcaaaaac
     5221 aggattgtcc ttatcgctgg cattctgatg attgccacaa cattacgcgt cacctttacc
     5281 ggcgcagcac cgttactgga tacgattcgt tccgcttact cgctgacgac ggcgcaaacc
     5341 ggcttattga ccaccctgcc attattagcc tttgcgctaa tctcaccttt ggctgccccg
     5401 gtggcgcgac gttttggtat ggaacgtagc ctgtttgccg cgttactttt gatctgtgct
     5461 ggtatcgcaa ttcgctctct cccttcgcct tacttattat ttggcggtac agcggtcatt
     5521 ggcggtggga ttgcattagg caatgtctta ctgccaggat taattaaacg cgatttccct
     5581 cattccgtcg ccagacttac cggcgcatat tccctgacaa tgggagctgc agcggcactg
     5641 ggatcgacta tggtcgtgcc gctggctttg aacggttttg gctggcaagg cgcgttgctc
     5701 atgctgatgt cttttcctct gctggctctt tttttatggc tgccacagtg gcgaagtcaa
     5761 caacatgcaa atttgagtac ctcacgcgcc ttacatactc ggggtatctg gcgttcgccg
     5821 cttgcctggc aggtaacatt gtttcttggg atcaactcac tggtctatta cgtgattatt
     5881 ggctggcttc cggcgattct catcagtcac ggctatagcg aagcacaggc gggttcactg
     5941 catggtttgc tgcaactagc cacagcagct cccggtttgc tgatcccact tttcttacat
     6001 catgtgaaag atcagcgtgg tattgcagcg ttcgttgcct tgatgtgcgc agtggccgcg
     6061 gttgggctct gctttatgcc agcacacgcg atcacctgga ctctgctttt cggttttggt
     6121 tccggcgcaa caatgatact ggggttgacg ttcattggtc tgcgggcaag ttctgcgcat
     6181 caggcggcgg cactctcggg gatggcacaa tccgtcgggt atttgctggc agcctgtggg
     6241 ccgccgctga tgggtaaaat acacgatgct aacggtgact ggtccgtacc actgatgggt
     6301 gttgcaatac tttcactact gatggcgatt ttcggacttt gcgccgggag agacaaagaa
     6361 attcgctaat atccggtgct atagtgacgt ggcaaatcat gcgtgaaagg gagaacaaat
     6421 acgatgaata ttcagtgcaa acgcgtttat gatccggctg aacagagcga tggttatcgc
     6481 gtactggtcg accgcctctg gccacgcggt atcaaaaaaa ccgatttagc ccttgatgaa
     6541 tgggataaag aaatcacgcc gtcaacggaa ctgcgcaaag cctttcacgg cgaagtcgtc
     6601 gattttgcaa cctttcgcga gcaatatctt gcagaactgg cgcaacacga gcaagaagga
     6661 aagcggctgg cgaacatcgc caaaaaacag ccgctgaccc tgctctactc agcaaaaaac
     6721 accacgcaga accatgcgct ggtgctggca gactggctac gtagcttgtg attttagtac
     6781 gccatccggc agttattttt caccagccgg atggtcccgt cgccacaatg cccattcatc
     6841 aatcgtttca ccgcccggta atttgcaatt gttgctgacc ccttgcgctg tctgcacagg
     6901 aatgagcgtc ccgcccttct gctggcaata gaccgacgcc ggatttgcca taccaatctg
     6961 cggcggttta ggtgcttctg gctgagaagg ggttgaacaa ccagccagga ccagcaagca
     7021 aggcagaaca aaactgataa ttttcattta ttgatctcac atatttatcc agacttagag
     7081 tatcgcggta tcgttttgtt ttgcagcact atttttatta cattcactca aatcatatta
     7141 cgccttgttt catctttgtt gatgatgttt tatcatgcct gcaaagatta aataatcagc
     7201 atttacccgc cgtatcctgg agttgtttcg tgtcagatca gattatcgcc cgcgtctcgc
     7261 aatcccttgc caaagaacag tcactggaaa gcctggtccg acagcttctg gagatgctgg
     7321 aaatggtcac tgatatggaa tcaacctacc tgaccaaagt ggatgtcgaa gcgcgcctgc
     7381 agcatataat gtttgcccgt aacagccaga aaatgcacat cccggagaat tttaccgtct
     7441 cgtgggatta ctcgttatgc aaacgcgcca ttgatgaaaa ctgctttttc agcgatgaag
     7501 tccctgaccg ttggggcgac tgtattgcgg cacgcaatct tggcatcacc acatttctga
     7561 gcacgccaat tcacttaccg gatggatcat tctatggcac gctttgcgcc gccagcagtg
     7621 agaagcgcca gtggagtgaa cgcgcggaac aggttttaca gttattcgcc ggactgattg
     7681 cacaatatat tcaaaaagag gcactggttg agcagctgcg cgaagccaat gctgcgctga
     7741 ttgcgcaatc gtataccgac tcgttaaccg ggctaccgaa tcggcgggcg atttttgaaa
     7801 atctgacgac gctgttttct ctcgcccgac atcttaacca taagataatg atcgcgttta
     7861 tcgatctgga taacttcaaa ttaatcaacg atcgttttgg tcataatagt ggtgatttgt
     7921 tcctcattca ggttggcgag cgccttaata cgctccagca aaatggcgaa gttattggtc
     7981 gtctcggcgg tgatgagttt ttggttgttt cactgaacaa cgagaatgcg gatatttcgt
     8041 ctctgcgaga acgtattcaa cagcaaatac gtggagaata tcacttaggt gacgttgatt
     8101 tgtattatcc cggtgccagt cttggcatag tagaagtcga tcctgaaacg accgatgcag
     8161 acagtgccct gcatgctgcc gatatcgcga tgtatcagga gaaaaaacac aaacagaaaa
     8221 caccttttgt cgcgcatcca gcgctacatt cctgaggcgt attcacatcc ttttgattgg
     8281 tgataacatg cgaatcggta ttatttttcc ggttgtaatc tttattacag cggtcgtatt
     8341 tttagcatgg ttttttattg gcggctatgc tgccccggga gcataaagat gaaaaaaaca
     8401 acgattatta tgatgggtgt ggcgattatt gtcgtactcg gcactgagct gggatggtgg
     8461 taacgtcaca tctagaaaat agcaaaggct gcctgtgtgc agcctttgtg caatttacgc
     8521 gttaactttt aatctttctg tagataaaca gcacgacaat cgcgccaata acggcaacca
     8581 cgaagctgcc aaaattgaag ccatcgactt taccaaagcc aaacagcgtg ctgatccatc
     8641 cgccgactac ggcaccgact atccccagca ggatagtcat aaagaatcca ccaccatctt
     8701 tacctggcat gatccacttc gccagaatac cggcaataag cccaaaaata atccatgaca
     8761 gaatgcccat tgtttcctca cttatctgtt ttgcattagc gggttagtcg ctgataaaaa
     8821 gcatagcaca acatcgtgag ggcaagattt gtgacgagca tcacggagga tttttttgcg
     8881 atggcgcaga aattgcgcca tcaacgatca gtgataatta ccaaccacaa acatcatgtt
     8941 cgttttccgt gtcataagaa cgcacggtat tcaccagatc ttttatcact tcagccgcca
     9001 cttctggcac cagcaaagtc atcggcgtct ctgtttcata atcgacagaa acgccattgc
     9061 tgttattggt gacggtcacg gtatacgttg ctttgcccat gattcatttc ccgttatgaa
     9121 tgactttccg ttgttgcgca ccttccatca ggacttcagg agcaacgaag aagtcaatgt
     9181 tgaaataagt gtcgtcagtc atggcttcaa tgttgtgcca cttttctgga gggaacacag
     9241 caaactgccc cgcttcgata aggatcactt gatcaggctc tgtactgtgt tcatcagcgt
     9301 agccgagata tttgaccgcc ccatgcataa cggaaaggcg tgggtaaacc cccgggcgcg
     9361 ttcctttatc aagatgacgt tcgaatattc cggcaggcgc agtttgttta ttccagaaag
     9421 gcgttgagcg cgtatgaata taattctgtg ggatttgcag catccttttc cctccttcgg
     9481 tgaatgcgct gaaaacggtt tatttcagcc gtttcagggt acgcctgata atttgcattt
     9541 taaataccat ttattggtta ctatttagca ccgtatcaga gaagaatcag ggaggattac
     9601 aggtgggaag cccgcgacaa cgcgggctga atgcatcagg actggagcgt cgccagtcgg
     9661 gcggcgaaac ccacaaacat caaaccaatc agtgagttgc ccactttagc cagtttcttt
     9721 ttggtacgta tgtactgcgt gacaaaagca ccagaaataa tcaggaagct caaatagcag
     9781 aaactcacca gttccagcgt cgtcgccaga ataaagaatg aaattcccgt atgtggggca
     9841 ttaacatcga taaactgtac gaaaaacgac acatagaaca aaatggcttt cggattagtc
     9901 aggctcaaaa ttaacgcgcg tttaaaaatg gcaccgtatt ggggctcatc ggatttggcc
     9961 tcgttatttt tacccttcag ggtcgcgtag agaattttac tccccagata gagcaaataa
    10021 aacgcaccaa gataacgtac aatgttgaat aatatcgggg tggtcttaat taatgtcgcc
    10081 actccagccc atgccagaaa catcaatacc gcatcgccaa taaatacacc gcaggccgca
    10141 agataaccgc ctttcatacc gctactgacg ctatttttga gtacaaacag ggtatttggc
    10201 cctggcacca acacaataaa aatggcccca accagatagg tccagtaatt cagaaccccg
    10261 tattcagcga acacattaac ctctttaatt atctttcgat catgcgcgat taaaggtgaa
    10321 tatgctaacc aatctgtagc ggcttagaaa ggagaaaatc aggttttaac ctgatatcaa
    10381 cccgataatt gaatcattaa tcaggcatgc tccagtgaaa aattcgggta gtgctcggca
    10441 aaatactggc gtaaaaattc taccgttatc cgtactttcg ctgacgtcgc cagccttgaa
    10501 acataaacgg cccagacatt cgctggctgg taatattccg gtagcacttg tactaaatga
    10561 ccactggcaa tgttttcgct aacatcccac caggagcgca gagcaatccc ctgcccatcg
    10621 agacaccact gatggacaat ttctccatga ttagacgaca gtgggccggt aactttaatc
    10681 gcatggtggc cttctttatt gcgtaattgc cagacaccaa aaggatgatc gcgctcttta
    10741 atgaccaggc acggcaatgc agaaagatcg gttaaatgtt tcggcgcgcc atgttgcgca
    10801 ataaattccg gtgacgcgca gagaatgcga taattggtcg ccagtttgcg ggcgattaaa
    10861 ttaggggcga tgtcatcgcc aatgcgaata tcgagatcga caccttcatt gaccaaatcg
    10921 accaaccggt cttccacatc aaaacgtaat tccagttgcg gatacgcctt cgccagcgct
    10981 gataacgccg gggccaccac ctgtcgtcca aaaccaaagc tgctgataat acgcagcatc
    11041 ccctgtggca cctgacgcac gtcagaaagt tcgtccatca tctgaccgac atcctgcaaa
    11101 atccgctgcg cccattcata aatccgctct ccttcttcgg taatggtgac gcggcgggtg
    11161 gtgcggtgta acaacaccac gtttagcgtt tgctccagca aggcgatgcg cttgctgacg
    11221 aacgccggag aaacgcccag ttcttccgcc acggcggcaa aaccagcccg gcgagccacc
    11281 agcataaaga cgcgcaaatc gttcagcagc ggtaaattat tcatgattcg tgttttatgt
    11341 ttcaccagtt acagggatta attccttttc agtcaattat aggatggtga tgttgtcaat
    11401 tttgatggtc aggaagtgag aacccaatga tgaaaacgat gcgtattgct gcgatcccgg
    11461 gagacgggat tggcaaagaa gtccttcctg aagggattcg cgtgttacag gctgccgctg
    11521 agcgctgggg cttcgccttg agttttgagc aaatggagtg ggcgagctgc gagtattaca
    11581 gccatcacgg taaaatgatg ccggacgact ggcatgagca acttagccgt ttcgacgcca
    11641 tctattttgg tgccgtcggc tggccggaca ccgttccgga ccatatttcg ttgtggggtt
    11701 cgctgctgaa atttcgtcgt gaattcgacc agtacgtcaa cctgcgcccg gttcgtctct
    11761 ttcctggcgt tccctgcccg ctggcgggaa aacagcctgg cgacatcgat ttttacgtgg
    11821 tcagggaaaa caccgaaggc gaatattcct cgctcggcgg cagagtgaat gaaggtacag
    11881 agcatgaagt cgtcattcag gaatcggtat ttactcgtcg tggtgtcgat cgtattttgc
    11941 gctatgcctt tgaacttgcg caaagccgcc cacgtaagac gctaacttct gccactaaat
    12001 cgaacggttt agccatcagc atgccgtact gggatgagcg agtggaagca atggctgaga
    12061 attacccgga gatccgctgg gacaagcagc atattgacat tctctgcgca cgttttgtga
    12121 tgcaaccgga acgcttcgat gtggtagtgg cgtccaattt gtttggcgat atcctttccg
    12181 atcttggccc ggcctgcacc ggcaccattg gcattgcccc atccgccaac ctgaatccgg
    12241 aacgcacttt cccgtcgctc ttcgagcctg tccacggttc cgcgccggat atctacggga
    12301 aaaaaattgc taaccctatc gccacaattt gggccggtgc aatgatgctc gattttctcg
    12361 gcaatggaga tgagcgtttc cagcaagcgc ataacggtat tctggccgcg attgaagaag
    12421 tgattgatca cgggccgaaa acacctgata tgaaaggcaa tgccaccacg ccacaggttg
    12481 ccgacgcgat ttgcaaaatt attttgcgtt aaggtcaaac cagtttattt gaaccgcgtc
    12541 actgacgcgg tttttttatt cgttctttgc agtaaataac ctgcgtcatt tcacctttta
    12601 ttgtttccgt ttcgtgtttt atggctttcc gtattcttaa ttgtttaatt tatgtaacat
    12661 gcaaataatc aatttgttaa tcacgacata caactcatta ccctgcaaat aattacaact
    12721 ggcaaaagcc agagacgggc ttttatgccc aggagatgac tcatgatgag caatgttaag
    12781 aaaaaagatg tgccgctgat aagcatcagc ctggtggcca ttcttttcat cgcagctgca
    12841 ttaagccttt tcccacaaca atcggccgac gcggccaacg caatatacac ttttgttacg
    12901 cgtacgttag gttccgccgt acaggtattg gttttgctgg caatgggact ggtgatttat
    12961 ttagccacca gtaaatacgg caatattcgt cttggcgaag gaaaaccgga atacagcacg
    13021 ctctcatggc tgtttatgtt tatttgtgcc ggtttaggtt cttctacgct ttattggggg
    13081 gttgctgaat gggcctatta ttatcaaacg cctggattaa atatcgcacc gcgttcacaa
    13141 caggcactcg aatttagcgt tccctactcc ttcttccact ggggcatcag cgcctgggca
    13201 acttatacgc tggcctcatt aatcatggct tatcactttc atgtgcggaa aaataaaggg
    13261 ctgagccttt ccggcattat tgctgctatt accggcgttc gcccgcaagg cccgtgggga
    13321 aaactggtcg atttgatgtt cctgatcgcc actgtcggcg cactgaccat ttcccttgtt
    13381 gtcaccgcag caacctttac ccgtgggctt tccgcgctga ccggtttacc cgataacttt
    13441 accgtacagg catttgtgat cctgctttcc ggcggcattt tttgcctaag ctcgtggatt
    13501 ggtatcaaca acggtttgca acgtctaagc aaaatggttg gctggggcgc gttcctgctg
    13561 ccattactgg tgctgattgt cggtccaacc gaatttatta ccaacagcat catcaatgcc
    13621 atcggcctga ccacgcaaaa cttcctgcaa atgagcttat tcaccgatcc gcttggcgat
    13681 ggttcattta cccgcaactg gaccgttttc tactggctgt ggtggatctc atacacgcct
    13741 ggcgtagcaa tgtttgtcac ccgcgtttcc cgcggtcgta agattaaaga agttatctgg
    13801 ggactgatcc tcggcagcac cgtcggttgc tggttcttct tcggcgtaat ggaaagctat
    13861 gccattcacc agtttatcaa tggcgtaatc aacgtcccac aggtgctgga aacactgggc
    13921 ggcgagacag ctgtgcagca agttctgatg tcgttgccag ccggtaaatt gttcctcgcc
    13981 gcatacctgg gcgtgatgat tattttcctt gcctcgcata tggatgcggt ggcctacacc
    14041 atggcggcaa ccagtacgcg taatctccag gaaggtgacg atcctgaccg tgggctgcgt
    14101 cttttctggt gcgtggtaat taccctgatc ccgctttcaa ttcttttcac tggcgcgtcg
    14161 ctggaaacga tgaaaaccac cgtcgtactc acagcccttc cgttcctcgt cattttactg
    14221 gtgaaagtcg gcggatttat tcgctggctg aaacaggatt acgccgacat tccggctcat
    14281 catgttgaac attatctccc gcagacaccg gttgaagtcc tggaaaaaac gccagtgctc
    14341 cctgcgggaa ccgtattcaa aggcgacaac tgagcgccat catcctaacg ataaaggtat
    14401 ccctatgagc aatctgagcc ctgactttgt actacccgaa aatttttgcg ctaacccgca
    14461 agaggcgtgg accattcctg cccgttttta taccgatcag aacgcctttg aacacgaaaa
    14521 agagaacgtc ttcgccaaaa gctggatttg cgtcgctcac agcagcgaac tggcgaatgc
    14581 caatgattat gtgacgcgtg agatcattgg cgaaagcatt gtgctggtac gcggtcgtga
    14641 taaggttttg cgcgcgttct ataacgtgtg tccgcaccgt ggtcatcagt tgttgagcgg
    14701 cgaagggaaa gcgaaaaatg tgattacctg cccgtatcac gcatgggcat tcaaactcga
    14761 tggcaacctg gcccatgcac gtaactgcga aaacgtcgcc aatttcgata gcgacaaagc
    14821 gcaactggtt ccggtgcgtc tggaagaata tgccggattc gtcttcatca acatggaccc
    14881 taacgccacc agcgtagaag atcagttacc cggtctgggc gcgaaagtgc tggaagcctg
    14941 cccggaagtc cacgatctga aactggcggc ccgctttacc acccgcacgc ctgccaactg
    15001 gaagaacatt gtcgataact atctcgagtg ctatcactgt ggtccggcgc atccaggttt
    15061 ctccgactcc gtacaggttg atcgttactg gcacaccatg cacggtaact ggacgctgca
    15121 atacggtttc gccaaaccgt cagaacagtc tttcaaattc gaagagggca cggatgcggc
    15181 attccacggt ttctggctgt ggccgtgcac gatgcttaac gtcacaccga tcaaagggat
    15241 gatgacggtc atttatgaat tcccggtgga ttctgaaact accctgcaaa actacgatat
    15301 ttacttcacc aatgaagagt taaccgacga gcaaaaatcg ctgattgagt ggtatcgcga
    15361 tgtgttccgt ccggaagatt tacgtctggt tgaaagcgta cagaaagggc tgaaatcgcg
    15421 tggctatcgt ggtcaggggc gcatcatggc cgacagtagc ggtagcggaa tttccgaaca
    15481 tggtatcgcc catttccata atctgctggc gcaggtgttt aaggactaat gacatcggcg
    15541 gcggtatttt ccgccgctgg gctgattttt gatggagtac agcaatgtca gactatcaaa
    15601 tgtttgaagt acaggtgagt caggtggaac cgcttaccga acaggtgaaa cgcttcacgc
    15661 tggtggcaac cgatggcaaa ccgctgcctg cgttcaccgg aggaagtcac atcatagtgc
    15721 agatgagcga tggtgataac cagtacagca atgcgtattc actactgagt tcaccgcata
    15781 acacctctag ttatcagatt gccgttcgac tggaggaaaa ctcgcgcggc ggttcccgct
    15841 ttttgcatca gcaggtaaaa gtgggcgatc ggttaacgat ttcaacgcct aataacctgt
    15901 ttgcgctaat tccctcggcc agaaagcatc tgtttatcgc gggcggtatt ggtatcaccc
    15961 ctttcctgtc gcacctggca gagttgcaat acagcgacgt cgactggcag ctacattact
    16021 gcacgcgaaa tccagaaagt tgcgcatttc gtgatgagct agtccagcat ccacaggctg
    16081 agaaagttca cttacatcac tcatcaaccg gaacacgact ggaattagcg cgattattgg
    16141 cggatatcga acctggcaca catgtttata cctgtggccc cgaggcgcta aatgaagcgg
    16201 taagaagtga agctgcgcgt ctgaacatcg ccgccgatac gctgcacttt gagcaatttg
    16261 ctatcgaaga caaaaccggc gatgcattta ccctggtgct tgcccgttcc ggaaaagagt
    16321 ttgtggtgcc ggaagagatg actattttgc aggttattga aaataataaa gcagcgaaag
    16381 tggaatgttt atgtcgtgaa ggggtatgcg gaacctgcga aacggcaata ctggaaggtg
    16441 aagctgacca tcgggatcag tattttagcg atgaagagcg tgccagccag caaagtatgt
    16501 tgatctgttg ttcgcgcgcg aagggtaaac gcctggtgtt ggatttgtag tttgccgaag
    16561 ccggactgag cgcgcccagt ccggcttcgg aagattttac tgcggatatt cctgcaataa
    16621 attgtgtaat gcttccgcca tcagctcacc acgccagccg gaaatcagct ccggcaaatt
    16681 gttctgcggt ttcagtttcc agtgccagtt cagcagttgg ttgatttgcc gacgcgatgc
    16741 cagcaattcg gcgctgatct tgtgcgtttc gctcacgtca gtaatcagcg acttaatcgc
    16801 tttaaacgct ttacgataac ccggcatgtc catcaggtta agcatcggct gcggtaaggc
    16861 ctcttccggc aatgcctgcg ctttttccac cagcgccagc agcgttttac cgtgaaagcg
    16921 gatttcgcta ccggataagc ccaggctgtc cagttcgcct aaacttcccg gcatataacg
    16981 cgctacggac cacaaatgct cttcacgcac gacaaagttc actgccagat cgcgctcccg
    17041 cgccttgcgc agtcgccagt cggctaacag ttgcagacag gccagttggc gtgtgcgtaa
    17101 ctgccaggca ttggtgatat cacgccaggc atcttccgga gcaacgactt cctgacgacg
    17161 catttgcatc aggcggcatt catccagcgc cgcaggtagc cagccggagg cctccgtttc
    17221 taccataagc tttgcggtga tcggtaacag ataccagaca tccgccgctg cgtattcaca
    17281 ctgacgttcg gtcagcggtc tggccagcca gtcggtgcgc gattcactct tgtccagcgt
    17341 aacgccggaa tactcttcca ccatggaagc gaaaccccat gacatcgggc gtccgcagaa
    17401 ggcagcaagg atttgcgtgt caatcaaggg ttgtggtaat tcgccaaaga cattgaggaa
    17461 cacttccaga tcttcactgc ccgcatggag aaattttgtg atggacggat cgcgcaggat
    17521 cgctttcagc ggtgaccagt cagagatccc gaggggatca attagcgcca gatgctcacc
    17581 atcaaaaagt tgaattagac ccagttgcgg gtaataggtg cgcgtacgaa caaattcagt
    17641 atccagggct atcgccggaa aggcacggac ggcttcacac aaagaagcca gcgcatcgtc
    17701 cgtggtaatc atttggtaat tcaaattgta ttctctttag tgggcgtcaa aaaaaacgcc
    17761 ggattaaccg gcgtctgacg actgacttaa cgctcaggct ttattgtcca ctttgccgcg
    17821 cgcttcgtca cgtaattctc gtcgcaaaat ttttccgacg ttagatttcg gtaactcatc
    17881 acgaaactcc accagcttcg gtactttgta gcccgtgagc tgacggcggc aaaaagtcac
    17941 cagtgactct tcggtaagcg atggatcttt tttcacaacg aagattttca ccgcttcacc
    18001 actggagccg gaaggtacgc caacagccgc gacttcctgt acgccaggat gctgcatgac
    18061 gacatcttca atctcgttgg gatagacgtt aaaaccggaa accagaatca tgtctttttt
    18121 acgatcgaca atgcgcagga atccttcttc atccatcacc gcgatgtcgc cggtgtgtaa
    18181 ccagccattt ttgatgattt catcggtagc atccggacgc tgccagtaac ccagcatcac
    18241 ctgcggtcct ttgacacaaa gctcacccgg ttgaccaggt ggtacttcac tatcatcatc
    18301 atccaccagt ttggcttccg tcgacggcac cggcaaaccg atgctaccac tatgataatc
    18361 aatatcatac gggttaacgc tcaccagcgg cgcacactcg gtcaggccat aaccttccag
    18421 cagatactgt ccagtcagtt taacccaacg ttctgccacc acttgctgaa caggcatccc
    18481 tccgcctgcg gaaagatgca gactggagaa atccagctgc tggaactctt tattgttcag
    18541 caacgcattg aacaaggtgt taacgccagt gatagcggta aacggatatt tcgctaactc
    18601 ttttaccaac cctggaatat cgcgcgggtt agtgataagc aggttctgcc cccccagttc
    18661 gataaacagc aggcagttaa tggtcagtgc aaaaatgtga tacagcggca gcgccgtcac
    18721 caccagctct ttgcccggat gcaacagcgg accataggtc gcgttaacct gttccaggtt
    18781 cgccagcata ttgcggtgag tcagcatcgc gcctttcgcc acaccagtgg tgccgccggt
    18841 gtattgcagg aaagctaaat cttccggcac cagttcgggt ttgacgtact gcatccggta
    18901 gccgttgtgc agggcgctac gaaatgaaat ggcatctggc agatggtatt tcggcaccag
    18961 acgcttgatg tatttaacaa cgaaattgac taccgtgcct tttgccgtag atagctgatc
    19021 gcccatacgg gtcagaatta cgtgctgaac ggcggtttta tcaaccactt tttccagagt
    19081 gtgggcaaag ttagacacga taacaatcgc cgatgcgccg ctatcgttaa gctgatgctc
    19141 aagttcacgc ggggtataca acgggttaac gtttacgaca atcatcccgg cacgcaaaat
    19201 gccaaacagc gccaccggat attgcaataa attaggcatc atcaacgcaa cgcgatcgcc
    19261 tttcttcagt cccaaccctt gttgcaaata agcggcaaac gcgcgactgc gttcttccag
    19321 tttgcggaag gtcatcacct cccccatatt cacaaacgcg ggttgatcgg cgtagcgcgc
    19381 gaccgactgc tcaaacatat ctaccagaga ttgataacgg tcagggttga tctccgtcgg
    19441 gacgtccgcg ggataacggt taagccaaac cttcttcaat tcttcacctc taaaatgcgt
    19501 gttcgtcgtc atcgcaaccc caaatgatat acatgctgtt aacataatat taactcatca
    19561 taccagcttg ataattaccc aacgaaaagg ttgcgaagcg cgtcactatt tatttttatc
    19621 tttaccgtaa gaatgcagaa acagcggacc agccgctgtt tctttttctt taaaaacaag
    19681 caattattca gttacaacag tttgtactct cgcggggccg ggattatacc agccccatcc
    19741 accgtagcca tagggccagc cacgaccgcc ataaaaccat ggatcaatcg gctgaggcgg
    19801 cataatcacc tgctgggtta aatgccaacg tttgtaaccc gttacctgca tcaccataaa
    19861 tttatagggc gtgttgccga ttttgccgtc aaccgcaccg gtgattggcc caactaccgt
    19921 aaccagttgt ccacggaaat ctaccgggtc cagaaaaccg ttcacatcag cataaatgcg
    19981 accgcgagaa ggttctccca gcgtcggtct ggctccgctg tccagtggta cggtggcaat
    20041 ttccaggcgg gttttccctt gctggttttg taccgcaacc actttgccac caaagcgtgc
    20101 ctcctgacca acgtacagct gcggcgcact catcacccga actaaatctt gttgcggcgt
    20161 gggactgctg cctttaatgg cgtccggcac agtaacacaa ccgctgagca ttagcgcaaa
    20221 cgtacctgcc agtatgcctt tgataacatt cttttgaacc gccatgatgc gactcctttt
    20281 ttctcagggc atactcttaa gattcattct ttgcccggaa gtttcttcca tgcgacgttg
    20341 ttacgtaaat aaaccggttc ggcatgttcc accgccaccg ttttaccctc ggcaaacatc
    20401 tgacacgcaa tcggaagcat atcttctgca gcgggcagta acacttcgcc atcgcacagg
    20461 gtcaaaccgc tctctttacc gagatccggc caggcttgcc agcctgtacc taccgtcacc
    20521 cattccccgg aaagctgttg catccgttca tgaacgagtt cgggtttgag tacggcttcg
    20581 gtttcttcac cgtgccagat acccttttca tcacgctgat attcggccca gtaaacttcg
    20641 cccatccgcg catcaatagc agccagcacg cgggttgcgc cgtttttgcg ccacgcccct
    20701 tgcgccattg tcatgagcgt ggaaacgcca atcatcggca gatccgcgcc aagcgccagc
    20761 ccctgcgcaa taccaatacc aatgcgcacg ccggtaaagc taccggggcc gcgcccgtaa
    20821 gccagagcgt taatatcagt cagggaagtt ccgctggtgg tcaggatatc ctgcaccatc
    20881 ggtaagattc gttgagtatg ttcacgaggg caaagctcaa aatgagcgtt gacagtaccg
    20941 tcgttccaca gggcgacaga gcaggcctct gtcgcggtat cgatagccag aattcgcatg
    21001 ggtcttcgtg cttagatcaa taaaaaggcg cgcatcatac catactccgt aacaaattac
    21061 ctggaggatg gtatcgcaag gaaacgaacc gcacgggcaa tgtcacgggt gcgcggcgct
    21121 ggcggcagac tggcgagaaa cgtcgcgccg taagggcgca tcaccagccg attatcgcaa
    21181 atcaccagca cgccgcgatc gtcggcgtcg cgaatcagtc gccctactcc ctgcttgaga
    21241 gtaatcaccg catccggcag ttgtacttca tcgaacgggt caccaccacg caaacggcaa
    21301 tcttccatgc gcgcttttaa tagtggatca tccggtgagg taaacggcaa cttgtcgata
    21361 atcaccaatg acaatgtatc gccacgcaca tccacccctt cccagaagct gctggttgcc
    21421 acaagaagcg cgttaccggc gctgacaaat tgctgcaaca gttgcccttt gctggtttcc
    21481 ccctgcaaca aaacgggaag cgtcatggta gcgcggaact gctcagccag atcgcgcatc
    21541 atggcgtgcg aggtacaaag cataaaacaa cgaccgttgt tagcttcgat gatcggtcgc
    21601 aacattgccg ccagttgccg tgcggagccc ggttgattgg tttgcggcag gttgcgcggc
    21661 acacagagta acgcctggcg gctgtaatca aacgggctgg gtaacagcaa cgactctgcc
    21721 tgttcaatgc caagccgcga ggtaaaatga tgcagatcgt cgttcaccga cagcgttgct
    21781 gaggtaaaga tccagctacc gggtttttgc gccattaact ctttgaattt atccgccacg
    21841 ctgagcggcg tgagcgccag agtaaaatgg cgcgaagtgc attcgtacca gtagctgtag
    21901 cccggctgat tgatctcttt tagccgtttc agccgcgtac gatacaacgt ggcgcgctca
    21961 aatgccgcat ccagcaaggc ggaacgcccc agcgacagtt tcgccacgtc ataacaaagt
    22021 tccagggtgt catcgagcag taaaaacgcc cgttgaattt gcggattagc taacagttcg
    22081 cgcaggttgc cacgataacc aggctcaccg agttgcagac gaaaatcctg cgcgctctgg
    22141 gcaaggcggt cggcgcactt ttgtaactgc tgagtatctt ttaattcggt gcgataggcg
    22201 atggtgatgt cttttgccag gtcgagtagt tgtcgactgg agagtgactg accaaaatac
    22261 tggctggcaa tgtcaggcag ctggtgggct tcgtcgaaga tcatgacgtc agcttccggg
    22321 atcagttcgc caaatccact ctctttcacc accatatccg ccagaaagag atgatggttt
    22381 accaccacca catcggcgtc catcgctttt ttgcgtgctt tgaccacaaa gcaatcttta
    22441 tacatcgggc agtcgctgcc aaggcagtta tcgttggtgc tggtgaccaa cggccacgcc
    22501 tgtgaatctt ccgccacgct gacgcaggtg ctgatatcac catcgactgt ttgattagac
    22561 caggagcgca gcaggatcac atcgcttaag atttgtaccg gcagatcgcc ccccgccagc
    22621 gcctgctgtt cgagacgttc gaggcagagg tagtttgagc gccctttcag caacgccacg
    22681 ttgcccgtat atttcaacgc ctttgaaacc gttggcaaat cgcggctgta gagctgatcc
    22741 tgcaacgctt ttgagccggt cgagataatg actttctttt tcgcccgcag cgcaggggcc
    22801 aggtaagcgt aggttttgcc cgtaccggtt ccggcttcca ccaccagcgg ctggcctttt
    22861 tctatcgcct gggtgacggc taccgccatc tgtcgctgtg gttctcgcgg cttaaagcct
    22921 ggtatcgctt tcgccagctg accgtctggt gcaaaatcgt ccgtcacact accccctgtt
    22981 gatttgaaca gggattatgt caggatgagg gcgctttcgc cagttgaagt ggtgacggcg
    23041 acctcacatt gtggcagtct ttgcagcacg aaaatggaag tataatgagg acaaaatgat
    23101 gactatcgtt cgtatcgatg ctgaagcccg ctggtctgat gtagtaatcc acaacaacac
    23161 gctctactac actggtgtac cggaaaacct cgacgccgat gcctttgagc aaaccgccaa
    23221 cacgctggcg cagattgacg ccgtgctgga aaaacagggc agcaataaat cgagcattct
    23281 ggatgccacc attttcctgg ccgataaaaa cgacttcgcg gcgatgaata aagcgtggga
    23341 tgcctgggtt gtcgcgggtc atgcgccggt gcgctgcacg gtacaagcgg gtttgatgaa
    23401 cccgaagtat aaagttgaaa ttaagattgt ggctgcggtg taagctttat cgaagcaaaa
    23461 taagtcagac gataatttat cgacaatact ggtcggtttt gcataaatcg accagaga
//
LOCUS       DAZUCC010000061        22483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZUCC010000061 DAZUCC010000000
VERSION     DAZUCC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.061"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            160..1857
                     /gene="ypdA"
                     /locus_tag="ACHY09_002080"
     CDS             160..1857
                     /gene="ypdA"
                     /locus_tag="ACHY09_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFS3885057.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1872..2606
                     /gene="ypdB"
                     /locus_tag="ACHY09_002081"
     CDS             1872..2606
                     /gene="ypdB"
                     /locus_tag="ACHY09_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFS3885058.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2619..3476
                     /gene="ypdC"
                     /locus_tag="ACHY09_002082"
     CDS             2619..3476
                     /gene="ypdC"
                     /locus_tag="ACHY09_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3885059.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPVRQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3479..5974)
                     /gene="ptsP"
                     /locus_tag="ACHY09_002083"
     CDS             complement(3479..5974)
                     /gene="ptsP"
                     /locus_tag="ACHY09_002083"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS3885060.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDGCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLNLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQMLADKRQQQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5999..7036)
                     /gene="ypdE"
                     /locus_tag="ACHY09_002084"
     CDS             complement(5999..7036)
                     /gene="ypdE"
                     /locus_tag="ACHY09_002084"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS3885061.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSTIRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7036..8121)
                     /gene="ypdF"
                     /locus_tag="ACHY09_002085"
     CDS             complement(7036..8121)
                     /gene="ypdF"
                     /locus_tag="ACHY09_002085"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS3885062.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVICR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIFADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQLKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGVEVLYAMPKTVLLTGEA"
     gene            complement(8136..9383)
                     /gene="fryC"
                     /locus_tag="ACHY09_002086"
     CDS             complement(8136..9383)
                     /gene="fryC"
                     /locus_tag="ACHY09_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS3885063.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9405..9731)
                     /gene="fryB"
                     /locus_tag="ACHY09_002087"
     CDS             complement(9405..9731)
                     /gene="fryB"
                     /locus_tag="ACHY09_002087"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS3885064.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9950..10915)
                     /gene="glk"
                     /locus_tag="ACHY09_002088"
     CDS             complement(9950..10915)
                     /gene="glk"
                     /locus_tag="ACHY09_002088"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFS3885065.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKIEVQDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11119..12375
                     /gene="yfeO"
                     /locus_tag="ACHY09_002089"
     CDS             11119..12375
                     /gene="yfeO"
                     /locus_tag="ACHY09_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFS3885066.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     QMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12490..12816
                     /gene="ypeC"
                     /locus_tag="ACHY09_002090"
     CDS             12490..12816
                     /gene="ypeC"
                     /locus_tag="ACHY09_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFS3885067.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12957..14195)
                     /gene="mntH"
                     /locus_tag="ACHY09_002091"
     CDS             complement(12957..14195)
                     /gene="mntH"
                     /locus_tag="ACHY09_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS3885068.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14531..15733
                     /gene="nupC"
                     /locus_tag="ACHY09_002092"
     CDS             14531..15733
                     /gene="nupC"
                     /locus_tag="ACHY09_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFS3885069.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15782..17971)
                     /gene="pdeA"
                     /locus_tag="ACHY09_002093"
     CDS             complement(15782..17971)
                     /gene="pdeA"
                     /locus_tag="ACHY09_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFS3885070.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLARMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18179..18254)
                     /locus_tag="ACHY09_002094"
     tRNA            complement(18179..18254)
                     /locus_tag="ACHY09_002094"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18219..18221),aa:Ala,seq:ggc)
     gene            complement(18294..18369)
                     /locus_tag="ACHY09_002095"
     tRNA            complement(18294..18369)
                     /locus_tag="ACHY09_002095"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18334..18336),aa:Ala,seq:ggc)
     gene            18590..18949
                     /gene="yfeC"
                     /locus_tag="ACHY09_002096"
     CDS             18590..18949
                     /gene="yfeC"
                     /locus_tag="ACHY09_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFS3885071.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18951..19343
                     /gene="yfeD"
                     /locus_tag="ACHY09_002097"
     CDS             18951..19343
                     /gene="yfeD"
                     /locus_tag="ACHY09_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003834896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFS3885072.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESE"
     gene            complement(19394..20809)
                     /gene="gltX"
                     /locus_tag="ACHY09_002098"
     CDS             complement(19394..20809)
                     /gene="gltX"
                     /locus_tag="ACHY09_002098"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS3885073.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            21068..21143
                     /locus_tag="ACHY09_002099"
     tRNA            21068..21143
                     /locus_tag="ACHY09_002099"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21101..21103,aa:Val,seq:tac)
     gene            21188..21263
                     /locus_tag="ACHY09_002100"
     tRNA            21188..21263
                     /locus_tag="ACHY09_002100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21221..21223,aa:Val,seq:tac)
     gene            21310..21385
                     /locus_tag="ACHY09_002101"
     tRNA            21310..21385
                     /locus_tag="ACHY09_002101"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21343..21345,aa:Val,seq:tac)
     gene            21390..21465
                     /locus_tag="ACHY09_002102"
     tRNA            21390..21465
                     /locus_tag="ACHY09_002102"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21423..21425,aa:Lys,seq:ttt)
     gene            complement(21576..22460)
                     /gene="xapR"
                     /locus_tag="ACHY09_002103"
     CDS             complement(21576..22460)
                     /gene="xapR"
                     /locus_tag="ACHY09_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFS3885074.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
BASE COUNT         5539 a         6020 c         5586 g         5338 t
ORIGIN      
        1 tttactaacc agaagtgcat tatcaagaga tgcattgcta atacaccatt tatctatcaa
       61 tcccctttta aaattgtggt ttttcctcaa aaacggcctg ctggtcatca gccttcaggt
      121 tacctatcat agaggtttaa tccttattca gagtcacccg tgcacgaaat attcaacatg
      181 ctgctggcgg tcttcgatcg ggcagcgtta atgcttatct gcctgttctt tctcatccgt
      241 atccgtctgt ttcgcgaact gttgcacaag tcggcgcact ccccaaaaga gttgctcgcc
      301 gttaccgcca ttttctcgct gttcgccctg ttcagcacct ggtccggcgt tcctgtagaa
      361 ggttcgctgg tgaacgtacg tattatcgcg gtgatgtcag gcgggattct gtttggcccg
      421 tgggtgggca tcattaccgg cgtgattgcg ggtattcacc gttatttaat tgatatcggt
      481 ggcgtgacgg cgatcccctg ctttatcacc agcattctgg cgggttgtat atcgggctgg
      541 atcaacctga aaatccccaa agcacagcgc tggcgcgtcg gtattctcgg cggcatgttg
      601 tgtgaaacgt tgaccatgat tctggtgatt gtctgggcac caactaccgc attggggatc
      661 gatatcgtct ctaaaatcgg gattccaatg atcctcggca gcgtctgtat cggctttatt
      721 gtgttgctgg tacaaagtgt tgagggcgaa aaagaggcca gcgccgcgcg gcaggccaag
      781 ctggcgctgg atatcgccaa caaaacgctg ccgctgtttc gccatgtcaa tagcgagtca
      841 ttacgcaagg tctgcgaaat tatccgcgat gacattcacg ctgacgcggt ggcaatcacc
      901 aataccgatc atgtgctggc ctacgttggc gtgggtgaac ataactatca gaatggcgat
      961 gacttcatta gcccgactac ccgccaggcg atgaattatg gaaaaatcat cattaaaaac
     1021 aatgatgaag cccaccgcac accagagatt cattccatgc tggtgatccc attgtgggag
     1081 aaaggcgttg tgaccggaac gctgaaaatt tactactgcc acgcgcatca gataacctcg
     1141 tcattacagg aaatggcggt cggtctgtca caaatcatct ccacgcaact ggaagtttcg
     1201 cgcgctgagc agctgcgtga aatggcaaat aaggcagagc ttcgcgccct gcaaagcaaa
     1261 attaatcccc atttcctgtt taacgctttg aacgctattt catcgtcaat ccgtctgaat
     1321 ccggataccg ctcgccagct gatctttaat ctgtcgcgtt atctgcgcta taacattgaa
     1381 ttaaaagacg atgagcaaat cgatatcaaa aaagagctgt atcaaattaa agactatatt
     1441 gccattgagc aggcccgctt tggcgacaag ctgacggtta tctatgatat tgatgaagag
     1501 gtgaattgct gcattcccag cctgctgatc cagccgttgg tggaaaacgc cattgtccac
     1561 ggtatccagc cttgcaaagg taaaggcgtt gtcaccatca gcgttgcaga gtgcggtaat
     1621 cgggtacgca ttgcggtgcg ggataccggg cacggcatcg atccaaaggt gattgagcgg
     1681 gtcgaagcga acgaaatgcc gggcaataaa attggcctgc tgaatgtcca tcatcgcgtg
     1741 aagttattgt atggcgaggg gctgcatatc cgccgcctgg aaccaggaac ggaaattgcg
     1801 ttttacattc ctaaccaacg cacccctgtc gcctcacagg ctacgttatt gctttgagcc
     1861 ggagtgatat tgtgaaagtc atcattgttg aagacgaatt cctggcacaa caggaactga
     1921 gctggctaat taaagagcac agccagatgg agattgtcgg cacctttgac gacggtctgg
     1981 acgtgttgaa gtttttgcag cataaccgcg tcgacgccat ttttctggat atcaatattc
     2041 cgtcgctgga tggtgtgtta ctggcacaaa acatcagcca gttcgcccat aaaccgttta
     2101 ttgtgtttat caccgcgtgg aaagaacatg cggtagaagc gtttgaactg gaggcgtttg
     2161 actacattct caaaccgtat caggagtcac gcattaccgg gatgctgcaa aaactggaag
     2221 cagcctggca gcaacagcag accagcagta cgcctgccgc gacggtaacg cgtgagaatg
     2281 acaccattaa tctggtgaaa gatgagcgaa tcatcgtcac gccaattaac gatatctatt
     2341 acgccgaagc gcacgagaaa atgacctttg tctatacgcg gcgtgaatcc tacgtaatgc
     2401 cgatgaacat taccgaattt tgcagcaaac tgccgccatc gcattttttc cgctgccatc
     2461 gctcattttg tgtcaatctg aacaaaatcc gcgagatcga accgtggttt aataacacct
     2521 atattctgcg gctgaaagat ctggattttg aagtgccggt cagccgcagc aaagttaaag
     2581 aattccgcca gttaatgcat ctttaatcgg gaactttcat gaaagcacca ggcttgcccg
     2641 ccgatcaaca atttttcgcc gatctgttca gcggcctggt gcttaacccg caactactgg
     2701 ggcgcgtctg gtttgccagc cagcctgcct cgttaccggt gggcagttta tgtattgatt
     2761 ttccccgtct ggatatcgtg ctgcgcggcg aatacggcaa tctgctggaa gcaaagcagc
     2821 aacgtatggt tgaaggagaa atgctgttta ttccggcgcg cgcggctaat ttaccgatca
     2881 acaacaaacc ggtgatgctg ttaagcctgg tgttcgcacc gacctggctt gggttatcgt
     2941 tttacgatag ccgcaccacg tcgttgttgc atccggttcg ccagatccaa cttcccagcc
     3001 tgcaacgcgg cgaaggtgaa gcaatgctta ctgccctcac ccatcttagc cgttcgccgc
     3061 tggagcagaa tatcattcag ccgctggtgt taagtttgct gcatctttgc cgtaacgtgg
     3121 tgaatatgcc gccgggcaat tcgcagccgc gcggcgattt tctctatcac agcatttgta
     3181 actgggttca ggataattat gcccagccgc tcacccgtga gagcgtggcg cagtttttta
     3241 atatcacgcc aaatcatctg tcaaaactgt ttgctcagca tggaacaatg cgttttatcg
     3301 agtatgttcg ctgggtacga atggcaaaag cgaggatgat tttgcaaaaa tatcatctgt
     3361 cgattcatga agtggcgcag cgttgcgggt ttccggatag cgactatttt tgtcgcgttt
     3421 tccggcgtca gtttggtctg acgccgggag agtacagcgc ccgttttcag ggctaacgtc
     3481 agaaggttaa ttctgtttcc agcagcgtca ggatgctttg tgcatcttgc gcggcaaaca
     3541 acgactggcg gaagttttta ttcaccagtt tacgcgccag ctgcgagaag actttcacat
     3601 gattcatccc ttcgttagcg cccagtgtca gcatgatcac cagttcgact tcgcccattt
     3661 ctgactgcca gtcaaccggt ttcaccagtc gggcaatgct gatactggaa tgacggatcc
     3721 actgagattt ggtgtgcggg atcgctacgc caaaaccaac gccggtggta acaatctctt
     3781 cccgctgcca gacatcctct tccagctcga acggatgttc agtgcgcccg ttaacgccga
     3841 ggttaccgca caggaactgg atcgcctgct ctttattgct aaaagcctga tcaacaaaga
     3901 tattctccag cgccagcaga gggcgaacgt cttcttccgg cgtaaacgcg gtgagtaacg
     3961 cttcaatctc ctgcgcgctg cggcattcac atgcctgacg cgccagttcc cgacacgcct
     4021 cgctatccag ttgacgaagc tggcttttca ccgccggaat gcgcgggcta ctcatactca
     4081 gctcgtccag gcccagccca agcagtagcg gcagataacg gctttcaccg cccagttcac
     4141 cgcaaatacc tacccattta ccccgctgat gcgcagtggt aacaatctgc tgcaacatgc
     4201 gcaggaacga tggcgtaatc gggttataca gcggcgatac gcgcgggtta ttacgatcga
     4261 ccgcatacag atactgggtc atatcgttgg agccgatact gaagaaatcg acctcatcgc
     4321 agaagtgatc gatgatgtag cacaccgacg gcacttccac catgataccc agcgtaatcg
     4381 tctctgcatg acgcaggcca tcgcgcttaa gctcaacgat cgctttttgg atctcgccct
     4441 tcacccataa gatctgatcg aggctgtgaa ccattgggat catcaactgg gcgttgccga
     4501 aactggcggc gcgcaaaatg gcccgcagtt gagtgcggaa caggccagca aattccgggt
     4561 aaattcgtac cgcacgatag ccgaggaacg ggttctcttc ctggggaatg ttgaggtaag
     4621 gaatgctttt atcgccgccg atgtccattg tgcggaagat aatcggcttg tcgcccgcgg
     4681 ccagtagcac ctgctggtag gcttcaaatt gctcctgctc gtccggcgca ctgtcgcgat
     4741 ccatatagag catttcggta cgaaacagcc cgacgccttc cgcgccgttg gcaaacgcgc
     4801 caggcgcttc cagcgcggtg ccaatattcg ccgcgatatc aatgcgcttg ttatcacggg
     4861 aataggccag ctgcgcggcg gcctgcgcct gctgctgctg acgtttatcc gccagcatct
     4921 gcgcaacctg ataataaccg ctcaccgcgt cattcgggtt aatcgccaac acgccgcact
     4981 gggcgtcaag caccgccggt tgcccggcat agcgcgcaat ggcatccagc ggcaggccgc
     5041 tcagtaccgg gatcgccgag gcgcgggcca gaatcagggt atgcgaggtg cgcccggttt
     5101 tctccagaat catgcccgca agatttttta aattgaggct caaaaactgg ctaggggtta
     5161 aatcctctgc caccagaatg gtcggttttt ccagcaccag attgttgcgc ggttttagtt
     5221 ccggccaggt gatatgcagt aactgttcgc tgatatcgcg aatgtcgcta acccgctcgc
     5281 gcagataatc actggcagaa gcagaaagtt tggcgcaaac ctgctccata ttgctgatga
     5341 tcgccgcccc cagcccctga tgctgttctg ccatcaggtg acggatattg cctgcaaatt
     5401 catcatcctg aatcagcgac aaatgggcgc tgaggatagt tttgctttcg ccgtcacgct
     5461 cacgcaattg ctggttcagt tgctcggcaa gcgttgccag gctgtgctcc agccgggtgg
     5521 aatcttgcgc actggcgggg atcgcccggt aactgtcgag gctgtcgctc tgtaacaggg
     5581 tcagcgtacc cacgccgacg ccgcttgcca gcacattgcc gtacagtaaa tcaggattca
     5641 gacggcttaa tgaacgcggc agcggatgcg ccgtcagttc tgcctgcgtt ggctggacgc
     5701 tgtcgctgtc gataaagcgc acctggatgt actcttccag cacgcgccgc gcctgctctt
     5761 catcgctgcc gctaatgttc aggctgcaac cgtcattaaa tagggtgccg gtgccaatca
     5821 gcgccagcga gcttttggca tctgcctttg cgttctggcg atggttaata aaagtgattt
     5881 cgctttgcca ctggctgcac tgttctttaa gttcccacgc cggacgagca tgcagaccgt
     5941 ttggcaaagg acagagaaat tgaatcgtta acataaccgc tccgggttaa tcaggagatc
     6001 atctgaaatc cgtcagttga acaaccgtct cacgcgtaag acgttgaata agggcagata
     6061 aaagctgctg catctgcaaa atgtcacggc aatcggcaat cgatgcggcg caatgtccgt
     6121 ggcgggtggc aggccccatc accactgtgg gtacgccagt gccggttaag tgcaccgccc
     6181 caccgtccgt gccaccgttg ctgaacatat ccgcctgcaa cggcacgcca atttctgccg
     6241 ccacggtttc aatccacgcg gtaagttttg gcggcgcaat cagcgattta tcgcttaaca
     6301 ccagcatcgg cccgttacca atctgacgat ggttagccgc gccataatca aagtttttcg
     6361 cccagcaggc ggtatcaagc acaatggcga cgtccggcga caccgcgcgg gtggcagttt
     6421 gcccgccgcg taatcccacc tcttcgctgg aacttgccac cagccacacc tccgcaggca
     6481 gttcggcgtc gtgcagctca cgtagtaacg tcaccagcag atagcaaccg aggcgatcat
     6541 caaaggcttt ccccatcacc cgctggtgag ggagaacctg aaaagttgta tcaaaggtga
     6601 cgcgatcgcc tggacgaatt cccgcctgca tcacttcgtc atacgagcgc gcaccaatgt
     6661 ccacgcgtat ggtgctgacg tcattcccct gccggtcgcc gtcaagcagg cctggaattt
     6721 tgcactcttc acgggtagtg atacgcaccg actgaagctg gcgggcagcc atgcgtacat
     6781 tgccaaccgg caacacatcg atcgcccctt cgcgggagat gctgcgcacc ataaatccca
     6841 cttcgtccat atgcgcacag atcatcacct tcggacctgt cgactcattg agtcggatca
     6901 gcaccgatcc cagcccatca aatcgcactt ctttttgcag gcgatccgct tcttccagca
     6961 ggatctgccg tacttcctgt tccgaggagg cgatcgcatc tgcctcgctc aacgctttta
     7021 ataacgataa atccattatg cctctcccgt gagcaacact gttttcggca tggcgtagag
     7081 cacttctacg ccttgcgggg tgaccagcac aacatcttcg atgcgcacgc ccccttgccc
     7141 tggcaaataa atccccggct ccacggtcag taacatgcct ggctgtagcg tcgtggtgtc
     7201 ccgcggtgaa aaacgcggat cctcatggac ttcaataccg atagcatgac cggtgttatg
     7261 accgaaatag tcgccataac cggcttctgt aatcacccgg cgcgcggcgt cgtcaacctg
     7321 ctggcagcgt acgccggggc gaatcgcgga gattgctgcg agctgtgcct gcaggacaat
     7381 ctgatagacg ttaaacagcg ggtgggattc ggcacttacc ccttcgccat tcaccagcaa
     7441 ggtgcgcgtc atatcagagc agtagccctg atacagcgca ccgaaatcga gagtgacaaa
     7501 ctcgcccgct gcaacaatct tgtcgctggc tttgccgtgc ggcagcgccc cacgccagcc
     7561 gctggcgaca atggtatcga aagaggcttt ttctgcgccc tgctggcgca taaaccactc
     7621 cagttcagcg gctatctcgc gctcgctcat ccccgcctga ataaagcggc gaatatgctc
     7681 tgcaccgcga tcggcaatcc cacaggcgag acggattttc tccacctcct ctggcgtttt
     7741 gagttgccgc agcacatccg gcgtggcgct aaccagtttc gcattgagtt cagactgcca
     7801 gcggtgcgcg gtttcccaac tcacctgctg gccttcaaaa ccgagcgttt gcaactgctc
     7861 atcggcaaag atttgcctga cgatggtggc aagcgtgtgc atcgcgtcga gcaaatgcag
     7921 ctggtaacct tgcgtgcggg cttctacatc cgcgtaatag cgcgagtcca ccagaatgtg
     7981 cgcactttca cggcaaatca ccacatagcc tgatccggtg gagatcccca gatgcggctg
     8041 tttgttctgc cgtgaggaga gaagcaccgc atccagttgt tgcgccttaa gccagtcgcg
     8101 cagcgaagcg agtaatgtca tcaggattgt ccttcttaca ggctatcgat taacaatttg
     8161 cctttacgga acatcatcag acgcaggaac accaccatca gcgcagttat gactgccccc
     8221 agcgcgatcc ccgccatgta gacgccgagg ttagtgacca gcggccatgc ccagatagcg
     8281 gactccggga accattgcac tgcgcccagc cagacagcgg cggttgagcc gacaatcgcg
     8341 ccgaccatat aggacggaat ggcggtgatg gggctttcca gcgcaaacgg aatcgcaccc
     8401 tcactgatcc ccataaacgc caggaacatg gcggttttac cctgcggata aagctgagca
     8461 ctgaacagac gtttgccggt taaacggcgg tcgataatgg tcgccagacc cagaccaatc
     8521 ggcggaataa caatagcgat ggaacgcgcg gtaaccggta atacgtggtc agtggtaaag
     8581 ctgaaggcaa cgaaaccagc tgctttgttg atcgggccac caaggtcgat tgctgtcgcc
     8641 gcggcgatcc ccatcgcgta cattagcgca cccttctcac ctgcggcagt cagcacggta
     8701 cggataccgc cgttgatcca gccaccaaag ggggtgatga cgtagtacat cgccagcatg
     8761 acaaaaatgg cggaaagaat cggtagcaaa aatgtggttt tgaacgctaa caggaaatcc
     8821 ggtaactgga ttttctggtt catccacttc acaaggtaac cagcaacgat agaaataatc
     8881 agggcaccaa tgaaggtgga cggcaccggc gaagaggtcg cccattgcat cgtgctggga
     8941 tcgaagttca gcagttgcgt cggctgggta gacatcaacc caccgataaa accagccggg
     9001 aacgccagtt tgccaccgat agagttcgcc acaaatgcag caaacatcgg aatggcaaaa
     9061 ccaaacaaca cgccgccgaa ggactgcgac aaccaggcaa atttcagtaa ggagaggtcg
     9121 aaaccggaga atttcccgct attaagagca tccatgatgc cgatatcagc aggaatttta
     9181 agccagctat aagcaatcag ctggctgaaa gcgaggatca caccgcccat aattaaggtc
     9241 ggcaccatgc gtgaaatccc tgacatcaca tgctgcggga gttcacccca gaagctggtt
     9301 ttagaggcct gcggattctt aactgccgct gccgcaccgg atgcgccagg cacaacggtt
     9361 gcactgcgtt ttttaatggc cataacaatt atccctgtcg aatattactg ttgttcagag
     9421 gcaatcatct cttcgatttc tttgatgatg cccgcagcgt ttttaattgc gtcctgcaaa
     9481 gtgatttcat aaacgtcgcg tgattcgaaa cgttcgttat cttccggggt aaccgccacg
     9541 gagtggatga tgatggtcgc ttcggcgata tcctgcgcgg tcaggcgatt ctggatgccg
     9601 tccgcgccct gagtttcaat tttcacttca tagcctgctt ccaccgccgc ttcttccagc
     9661 gcctgggctg ccataaaggt gtgagccaga cccatcgggc aggcacataa agcaatcagt
     9721 ttcttactca tcgttaattc ctcgttcagt gatagttgac gagtgaaatt atgaacgtgc
     9781 aaaacaaatc gccgttacca gacaaataat gcatttactg gagaattaat cccgctgtac
     9841 ggaagtgtga cgcaggtcgg ccttgtgagg ggacacaaag gaaaattgaa ttaactgtat
     9901 gatttaaaag attatcggga gagttacctc ccgatataac aggaaggatt tagagaatgt
     9961 gacctaaggt ctggcgtaaa tgtgcaccgg aaccaagaag gcccggattg tcatggacga
    10021 tgagatacac cggaatatca tggacatatt ctttaaagcg ccctttatct tcaaatgcgg
    10081 cacggaaacc agaggctttg aagaactcaa ggaagcgcgg cacgatacca cccgcaataa
    10141 acacgccgcc aaatgtcccg agattgagcg ccagattgcc gccaaaacgg cccataatga
    10201 cgcaaaacag cgacaacgcg cggcggcaat cggtgcagct gtcagccagc gcgcgttcgg
    10261 taatatcttt tggcttgaga ttttctggca ggcggttgtc ggctttaaca attgcgcgat
    10321 acaaattcac cagcccaggg ccagaaagca cgcgctctgc cgaaacatga ccaatttctg
    10381 cacgcagtat ttcgaggata atgccctctt cttcactatt cggcgcaaaa tcaacgtgac
    10441 cgccttcgcc tggcaagctt acccaacgct tatcgacatg gaccagatgc gcaaccccta
    10501 gccccgttcc ggcaccgtaa accgcaatag gcttaccttc taccggttct gcgccaccaa
    10561 actgaatcag atgctctttt ttcagcatcg ggatcgccat cgatacagcg gtaaaatcgt
    10621 tgataatttc cagatggcta aaaccgagat tctttttcat ttcggcaatt gagaacgccc
    10681 aggtatggtt ggtcattgcc acccagtcac cggtaattgg gcaagcgatg gcaatacagc
    10741 cgtcttgcac ctcgatctta tgttcttcaa gataaacgcg aatgaccgct tcgaggctgg
    10801 ggtaatcaag ccctgaatag gtcttagcct gcgagatttc accactggca atatcacaca
    10861 gagcaagacg tgcgttggtg ccgcccacat caccgactaa tgcatacttt gtcattcttc
    10921 aactgctccg ctaaagtcaa aataattctt tctcacactg taaatacctg ggggcataac
    10981 aacaacgccg gaaaggcagg ctccctgtaa atatcgatct gggtcacaca attactttat
    11041 cgtttcagca ccaattgcag cgatgccttt ttgcaagctg ggcaaactaa gtatctgacc
    11101 ccgcataagg aatagaacat gctccatccg cgagccagaa ccatgttgtt attatcgctc
    11161 cccgccgtgg caattgggat tgcgtccagt cttattctga ttgtggtgat gaaaatcgcc
    11221 tcggtattac agaatttgct ctggcaacga ctgccgggaa ctctggggat agcccaggat
    11281 tcacctctct ggatcatcgg tgtattaacg ctaacgggta ttgcagtggg gttggttatc
    11341 cgtttcagcc agggtcatgc cggaccagac cccgcctgtg aaccgctgat cggcgcacca
    11401 gttccgccct ctgcgctacc aggacttatc gtagcattaa ttcttggtct tgctggtggc
    11461 gtcagcctgg ggccagaaca tccgatcatg accgtcaata tcgcccttgc ggttgccatt
    11521 ggcgctcgtc tgttaccgcg cgtcaaccga atggagtgga ctattttagc ctctgccgga
    11581 accatcggcg cgctgtttgg cacacctgtt gcggcggcgt tgatattttc gcaaacctta
    11641 aatggcagta gtgaagttcc gctatgggat cgtctttttg cgccgttaat ggcggcagca
    11701 gctggtgcac taactaccgg attatttttc catcctcatt tttcactgcc cattgcgcat
    11761 tacggacaga tgcaaatgac cgatattctc agtggtgcaa ttgtcgcggc gattgccatc
    11821 gcagcaggga tggtcgccgt atggtgctta ccacgattgc acgcgatgat gcatcaaatg
    11881 aaaaatccgg tgctcgtgct gggtattggc ggatttattc tcggtattct gggggttatg
    11941 ggtggaccag tttcgctgtt taaagggctg gatgagatgc agcagatggt ggcaaatcag
    12001 gctttcagca ccagcgatta ctttttgctg gcggtgatta aacttgccgc cctggtcgtt
    12061 gctgccgcca gtggctttcg cggtgggcga atcttcccgg cagtgtttgt cggcgtggca
    12121 ttagggttga tgctgcatga gcacgtcccc gccgttccag cggcaataac cgtttcctgc
    12181 gctattctcg gcatcgtgct ggtggtaaca cgcgatggct ggttaagtct ttttatggcg
    12241 gcagtcgttg tacccaatac cacattgcta ccgctgctct gtatcgtcat gcttccggca
    12301 tggctgttat tagcaggtaa gccgatgatg atggtcaatc gtccgaagca acagcccccc
    12361 cacgataacg tttagcaaaa aatgctctcc tttatgattt aagagttatg gcgatgatac
    12421 ggggctttac ctccccgtaa tattgcgtta acaggccgct gacggcttcg atcgtgaagg
    12481 agaataacaa tgttcaggtc actgtttctg gcggccgccc tgatggcatt taccccgctt
    12541 gcggcaaacg caggtgaaat caccctactg ccatcaatca aattacaaat tggcgatcgc
    12601 gatcattacg gtaattactg ggacggtggt cactggcgcg accgtgacta ctggcatcgc
    12661 aattatgaat ggcgtaaaaa ccgctggtgg cgtcatgata atggctacca ccgtggctgg
    12721 gataagcgta aagcgtatga gcgtggctat cgtgaaggct ggcgcgatcg tgacgatcat
    12781 cgcggaaaag gccgcggaca tggacaccgc cattaaacac attcgccaat ggagcacaat
    12841 gcctgatgcg acgctagtgc gtcttatcag gcctacaaac cggctcttta acgcgtaggg
    12901 cggataaggc gtttacgccg catccgccaa tagtgccaga tgcgacgctc attcaactac
    12961 aatcccagcg ccgtccccac caacaaccag atattcagcg ccacgaccag cactacaatc
    13021 acccagcctg tctgttttac gcgtttgctg ttcaccagat cgcccatcaa cttgctgtca
    13081 ctggtgaaaa tcagcagtgg aaccagcgcc agagcgatac caaaacttaa cagcacctga
    13141 ctcataacca gaatccgtgt cggatctaat cccatcagaa tgacaataaa tgacggcaac
    13201 atggtaacgg tgcgacgcac ccacagcggg atatggaagc gaatgaagcc ctgcatcacc
    13261 acctgccccg ccagtgtccc caccaccgtt gatgacagtc cggcagcaac caggcttaat
    13321 ccaaagaccg ttgccgcagc atggcttaac aacggttgca gcgtcaggta agcctcatca
    13381 agatcggcaa cgccagtatg accggaaaag tggaacgccg ccgcagccgt agccatcatc
    13441 gccagattga caaaaccggc aatagtcata gcgatagcca catcccattt ggtggcagaa
    13501 taacgttgtt gacgcgaacc accatgtaaa tgctgagtca gcgaagagtg taaataaatc
    13561 acgtgtggca taatcgtcgc ccccaacaca cctgccgcca ggaagactgc ctccgaggtg
    13621 ggtaaactcg ggattaccat tcctttaccc agttgcgcca ggttaggctg ggagaaaatc
    13681 aactcgacaa tgtaagccgc agcaacaaac agcagtaacc cgccaatcac tttctccagc
    13741 ggtttttgcc ctcgacgttg cagcattaaa atcaggaaag tcgcgatccc cgtcagcacc
    13801 gcgccctgca acagcgaaac acccagaatg agtttaaaac cgatcgccgc accaataaat
    13861 tccgccaggt cggttgccat cgcaatgatt tctgcctgaa cccaatagaa ccacactacg
    13921 ggacgcggat agtgatcgcg aatctgctcc gccaggtttt taccggtggc aatccccagt
    13981 ttggcagaca gtatctgaat cagcatcgcc atcaggttgg cccaaacgac aacccacagt
    14041 agctgatagc cgaagctggc acccgcctga atattggtcg caaagttacc aggatcgata
    14101 taaccaatcg ccgcaatgaa cgcaggtccc attaatgcga gcctcatctt gcgcgccgcc
    14161 cgtccgctgc tactctcaac gcgatagtta gtcatcttgt gcctctaaaa catagccttt
    14221 gctatgtttc atgctatgtc aaacgagaat gattatcaaa ttcatttaag tggattgtga
    14281 tgatttcttt gatagaccag aattatgacg acgaaaagat tgatgacttg aatgttgtga
    14341 ttatgtttaa tgttagcaca tttacataac tttcagcttc catacacaac atagcagaaa
    14401 tgtatgacag ctcactattt ttgaagcttg tcacaggacg tcattatagt gtgtgtcaga
    14461 tctcgttttc cttaaccatg ttacatagaa tgtgcacgga aatttaacct gcctcatatt
    14521 tggagcaaat atggaccgcg tccttcattt tgtactggca cttgccgttg ttgcgattct
    14581 cgcactgctg gtaagcagcg accgcaaaaa aattcgtatc cgttatgtta ttcaactgct
    14641 tgttatcgaa gtgttactgg cgtggttctt cctgaactcc gacgttggtt tgggcttcgt
    14701 gaaaggcttc tccgaaatgt ttgagaaact gctcggattt gccaacgaag ggactaactt
    14761 cgtctttggt agcatgaatg atcaaggcct ggcattcttc ttcctgaaag tgctgtgccc
    14821 aatcgtcttt atctctgcgc tgatcggtat tctccagcac attcgcgtgt tgccggtgat
    14881 tatccgcgca attggtttcc tgctctccaa agtcaacggt atgggcaaac tggaatcctt
    14941 taacgccgtc agctccctga ttctgggtca gtctgaaaac tttattgcct ataaagatat
    15001 ccttggcaaa atctcccgta accgtatgta caccatggca gcaacggcga tgtccaccgt
    15061 gtcgatgtcc atcgttggtg catacatgac catgctggaa ccgaaatacg tcgttgcagc
    15121 gctggtactg aacatgttca gcacctttat cgtgctgtcg ctgatcaacc cttaccgtgt
    15181 tgatgccagt gaagaaaaca ttcagatgtc caacctgcac gaaggtcaga gcttcttcga
    15241 aatgctgggt gaatacattc tggcaggttt caaagttgcc attatcgttg ccgcaatgct
    15301 gatcggcttt atcgccctga tcgccgcgct gaacgcactg tttgctaccg tgactggctg
    15361 gtttggctac agcatctcct tccagggcat cctgggctac atcttctatc cgattgcatg
    15421 ggtgatgggt gttccttcca gtgaagcact gcaagtgggc agtatcatgg cgaccaaact
    15481 ggtttccaac gagttcgttg cgatgatgga tctgcagaaa attgcttcca cgctctctcc
    15541 gcgtgctgaa ggcatcatct ctgtgttcct ggtttccttc gctaacttct catcaatcgg
    15601 gattatcgca ggtgcagtta aaggcctgaa tgaagagcaa ggtaacgtgg tttctcgctt
    15661 cggtctgaaa ctggtttacg gctctaccct ggtgagtgtg ctgtctgcgt caatcgcagc
    15721 actggtgctg taagaccata cataaaaaag ccggggataa ttccccggtt ttttacgcct
    15781 gttaatcagc taatggctgc gggcgaccaa tcaaataccc ttgcagatat tgcaccccga
    15841 gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa ctccgcgacc acactcaatg
    15901 acttcgcttt cgccagatcg gtaattgatc tcacaatcat cgcatccagc gtgttcgtga
    15961 cgatatcttt cacaaagacg ccatcaattt tgatgatatc agcctgcaaa cgctttaacc
    16021 gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc aatccggaaa ccaaacttat
    16081 gcagctgctc gatgttgtac atgctgcttt ctgcgttaga aaacgcctgc tcctcggtga
    16141 tctcaagaat gaccgcctgc ggggagatgt gataacgctt aaacagacga ataatccgcc
    16201 cggcaatatt cttttgcagc agcgtgagcg gcattaaatt gactgaaaag cgcgggcctt
    16261 ttttgtcgca aggatgtgtt gccagccact tcaacaggga ttccagcact tgcaaatcaa
    16321 aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa cttatccggg gtcataatgc
    16381 cgccgtcata tttcagtcgc gcgaggatct catcataacc ttcaccctct ttgttgcgaa
    16441 ttggctgggc gtagagcagc aaatcaccct gatctaacgc cgtacgaatg gtattcagca
    16501 atagcacctg tttagtggtc tgcccggaaa ccatctcctc tctgctatcc agcgccagca
    16561 catggtgatg tgcgcaggat tgctccgcca gccagcttaa ctgccccaac aagggttgca
    16621 gggtttcctg atttccatca aaacgccccc aggcagcacc atagcccata tccagcccgg
    16681 tattgttcca gtgaatttgc cgactattca ggatgttaac catatgctgg agtcgccctt
    16741 ccgtttccgg tccactcagc actaacagca gttcactacc cggcaattga tacaactttt
    16801 cgttttcctg catcaacggc agcagcgtac ggtaaattga tcggatacag tgaacgcgca
    16861 tcattaagcc gtaatgacga ctcataaact caagattatc aatgcgcagg cagcaaaaac
    16921 tcttgcccgc ctcttgctcc ggcgcttgtt ctaacgcacg aaagttgggt aggagcgtta
    16981 acggatcggt cagcgcctgc aaatgccagc ggcgattaag ccattcactg cgatgataaa
    17041 tgcgcaccat atagagcagg caaacgctaa aggaaatcag caccgcgaga ataaatgcca
    17101 gcgaatattc ggttagtacc ccttgcaaaa agttctggtt gtaattcagc aggcaaagcg
    17161 ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag cttaccgaca ccgagggtaa
    17221 agatgataaa gaaaacaggt accagataac cagcaataaa gtcattttca tacggtgtgc
    17281 acatcagaag tagcagcacg ctaagagctg ccagccagct taaggtaaat gcgcgtttct
    17341 ctttgctcaa cgacggagcg atatccctac gccacaatat ctgtgcaaag tggggactta
    17401 caatcatgcg ggcgagatag taaaaaagca tgttgtaaat cagcactgcg gtgaaaaggc
    17461 ttagcaaatc aacgaccgtg aaaatggcat ccgcatcgcc gaaaaaggta gatatcttta
    17521 gcggaaaatc aaagaaattt cccacaagat acatgctgca tttgatgcca atcggcgcca
    17581 ccaaaccaag ccagaaaaga cgttgccaga catatcggct ggtcaatcca taacgccaac
    17641 gcgtccccag ctgccagcgt aatattgcgc aggcacacaa caccacaaac gtctggctgc
    17701 cgagcatgac cgcggtctgt aaaaaagaga gatgaaagtt ccacagattg gtgcaaaaca
    17761 tcccgcataa aacggggacg acaccgcgcc agccaaagat aaacaagatt gacagcatta
    17821 cgcacagtgg catccacgcc agaaagatat aactggagtg gataatggca agtggcgaaa
    17881 taaaacggga tagctgaata agtaccacgg tgagcgtaaa cgctagtgtg aatatcttga
    17941 tattttttat caggttatgc tccacaaaca tgagtacact actctcactg atcggggttt
    18001 tcaggcacac aaataataag tttattcgca tcgcgaagca aacaggggcc agcctgttgc
    18061 cgatttttca tccaatgatg ctttcaccag gcggcgctaa cccggacgta gactgtatta
    18121 caaaagcggc aaaaagcaga gacaaaaaac ccccgcttta cagcgagggt ttgaaatttg
    18181 gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc
    18241 tgagctatag ccccacgatg cgtttacgta ccaaatttgc tgggtgcaaa atttggtgga
    18301 gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc ccagctgagc
    18361 tatagcccca tcacgtaaag cttgtcgagt tgacgggcgg catcatatga attccgcccg
    18421 aatgtgtcaa cggcaaattg ccacgtgtag tttcaatcgc tgaaaaatca ggcaaatgaa
    18481 caattttgga aaaccgctcg cattcagtag ttattcatgt cacggtttcc tgtaaagtgg
    18541 tgttataaaa tgaactacta atagacccac atacattcag ggaattgtta tgttcaagga
    18601 gaggatgacg ccagatgaac ttgccagact gaccggctat agccgccaga ccattaataa
    18661 atgggtacgc aaggaaggct ggacaacgtc accaaaacca ggcgtccagg gtggcaaagc
    18721 tcgactggtt cacgtcaatg aacaggttcg tgaatatatt cgcaatgctg aacgtccaga
    18781 aggtcaggga gaagcgccag ccctttctgg tgatgcgccg cttgaagtgt tgctggtgac
    18841 actggcgaaa gagatgacgc cagttgaaca aaaacagttt acatccttgc ttctgcggga
    18901 agggattatc ggattgttac aacgcttagg gattcgcgat agcaaataat atgaaaagat
    18961 tacgcaataa aatgaccacc gaagaactgg ctgaatgcct cggtgtggct aaacaaactg
    19021 ttaaccgttg gatcagagaa aaaggctgga aaacggaaaa atttcccggc gtgaaaggcg
    19081 gtcgtgccag acttattctg gtcgatacgc aagtttgcga gtttattcag aacacgccag
    19141 ccttccataa cacaccgatg cttctggaag ccgaagaacc ccttgccgaa tacgcaccgg
    19201 gtattcgcac tcctgcttat cggcaaatca tcagcgcaat agacaacatg actcacagtg
    19261 agcaggaaaa agttgcgcaa tttttgtcac gcgaaggaat tcgtaatttc cttgctcgtc
    19321 tcgacataga tgaatcagaa taaataaaaa acggcagggg tatctctcct gccgtttatc
    19381 ttttatgcgc taattattgc tgattttcgc gttcagcaat aaaagccagc gctttgttga
    19441 tacgctcgat actgcgggtc ttaccgattg cgtgaacggt aacatccagc gccggagact
    19501 gccccgcacc ggttacggcg acacgcagcg gcataccaac tttacccata cccacttcca
    19561 gctcatccgc cgtcgcctga atagcgtgat gaacgttttc agcggtccag tcagtaatcg
    19621 cagtcagttt gtcacgaacc acttccagcg gctgacgcgc taccggacgc agatgttttt
    19681 tcgcggcgtc ggcatcgaac tcagcaaaat cttcgtagaa ataacggcag ctctgtgcca
    19741 tctctttcag cgtcttgcag cgttcgccca gcagtttcac cagatcagcc agctgcgggc
    19801 cgttacgggt atcgatattt tcctgctcaa tgtgccattg taagtgagta gcaacatact
    19861 ccggcggcag cgcgttaatg tagtgatggt tcagccacag cagcttgtcg gtgttgaacg
    19921 cactggcaga tttgctgacg gcattcagag tgaagtattt gatcatctct tcacgagtga
    19981 agatttcctg atcgccgtgg gaccagccca gacgcaccag atagttcagc agtgcttctg
    20041 gcaaataacc gtcatcacga tactgcatta cgctgaccgc cccgtgacgt ttggacagtt
    20101 ttttaccgtc atcgccgttg atcatagaaa cgtgcgcgta aaccggtacc ggcgctttca
    20161 gggctttaag aatgttgatc tggcgcggcg tgttgttgat atggtcttcg ccacggataa
    20221 catgggtgat ttccatatcc cagtcatcaa caaccacaca gaagttatag gttggggaac
    20281 catcggtacg gcgaatgatc agatcatcca gctcctggtt gctgaactca atcgggccac
    20341 ggatctgatc gtcaaaaaca acagaacctt cctgcgggtt agcaaaacgt acaacgcacg
    20401 gttcatcatc agcatgatgc tcatggctgt ggcggcagcg accgtcataa cgcggcttct
    20461 cacctttcgc catttgctct tcgcgcagcg cttccaggcg ctctttagaa caatagcatt
    20521 tataagctgt gccctcttcc agcatctgat cgatcaccgc gttgtagcga tcaaaacgtt
    20581 tggtctggta gtacggacct tcatcccact ccaggctcag ccagttcatg ccatccataa
    20641 tggcttcgat agcttccggc gtggaacgct caagatcggt gtcttcaata cgcagcacga
    20701 actcaccgcc gtggttacgt gcaaaaagcc aggagtaaag agcagtacgc gcgccgccaa
    20761 cgtgcagata gcctgttggg cttggcgcga agcgagtttt gattttcatg aaatggcctt
    20821 acgtttagaa agatgccgac aaccggcaaa tcctggaaaa attgagtggg cgatattcta
    20881 tcactcccgc ctgattcctc aatgtagttc gggttgcaac cttacgcatt attgttttcg
    20941 ttgacaaatt gcgcaacacg gtctgttttg cgatcatctt gcttaaattt acgacgaacg
    21001 aacaatttct ttagaaaatg cgttgactca ttttgaactc tccctataat gcgactccac
    21061 acagcggggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    21121 ttcgatcccg tcatcaccca ccaactactt tatgtagtct ccgccgtgta gcaagaaatt
    21181 gagaagtggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    21241 ttcgatcccg tcatcaccca ccactttctc gccagctaga tttcttgtaa caatgtgaag
    21301 taccgaagtg ggtgattagc tcagctggga gagcacctcc cttacaagga gggggtcggc
    21361 ggttcgatcc cgtcatcacc caccacttcg ggtcgttagc tcagttggta gagcagttga
    21421 cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccaatgta aaaaagcgcc
    21481 ctaaaggcgc ttttttgcta tctgcaattt gcgaaattgc ctgatgcgct tcgcttatca
    21541 gaccaactat gtccggcagt tcccgtgcct ctcatctact gcgccaacgc cgccagcagt
    21601 ttaaccagcg cgggcgtcac ctgctgcgtt tcataaacaa tatataagtc tgccgggatg
    21661 cgttctttga gcggacggaa tatgacaccg ggccagttca tctgcgcgta gctgtccgct
    21721 atcaatgtga taccaatacc catactgacc atggcgagta ccgtttgcgg ttcattaact
    21781 tcgcgaataa caaccggtga aaatcccacc tgctggcaaa ctcgctgcaa aaaatcccag
    21841 tcagtgtaca cgggcggcat tgtaacgaaa tactcgtcac gtagcgcttc cagcgggacg
    21901 gtggaaaatg atgagagatg atgctcttca ggcatcgcca ccagaaacgc cgattcatgc
    21961 aaccgtaagc tggtaaaacc agtcggtggt tctgtcgcca ttcgccagat cccggcatca
    22021 agttcgcggc gttccagcaa ggccatttgc atcgcaggca tcttttcgcg aaacagaact
    22081 tcaacgttag gattttctct aaggaatcgc cgcataaccg ggcgcatccg tccccacatt
    22141 gccgttccca caaccccgag ttcaatccgc cctgcttctc cccgacctat ttgttcaacc
    22201 cgagccaata cattatttgc attcaccagc aatcgacgcg attcttccat caagattttg
    22261 cccgcgtgtg tcagtacgac gctgcgcgaa tggcgaataa aaagctgcgt gccgagttga
    22321 ttttccagct ctttaatatg aatgctgagc ggaggctgag acatatttaa acgcgctgct
    22381 gcgcgaccaa aatgcaactc ttccgctacg gcaagaaaat aacggagcaa cttaagatct
    22441 gttctgtata cgcgttccat aattaagtgc tccatataat aaa
//
LOCUS       DAZUCC010000062        22419 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZUCC010000062 DAZUCC010000000
VERSION     DAZUCC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.062"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..573
                     /locus_tag="ACHY09_002104"
     CDS             <1..573
                     /locus_tag="ACHY09_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS3885075.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGSSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQ
                     AALTYRYGNEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            912..1385
                     /locus_tag="ACHY09_002105"
     CDS             912..1385
                     /locus_tag="ACHY09_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS3885076.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFSLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1401..1877
                     /gene="yeeS"
                     /locus_tag="ACHY09_002106"
     CDS             1401..1877
                     /gene="yeeS"
                     /locus_tag="ACHY09_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFS3885077.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1946..2167
                     /gene="yeeT"
                     /locus_tag="ACHY09_002107"
     CDS             1946..2167
                     /gene="yeeT"
                     /locus_tag="ACHY09_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS3885078.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2330..2698
                     /locus_tag="ACHY09_002108"
     CDS             2330..2698
                     /locus_tag="ACHY09_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFS3885079.1"
                     /translation="MSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            2788..3165
                     /locus_tag="ACHY09_002109"
     CDS             2788..3165
                     /locus_tag="ACHY09_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFS3885080.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSTSPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKR"
     gene            3162..3650
                     /locus_tag="ACHY09_002110"
     CDS             3162..3650
                     /locus_tag="ACHY09_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS3885081.1"
                     /translation="MKLSLTLEADSINVRALNMGRIVVDVDGVELAELINVVCDNGHS
                     LRVVDESDRASTDCTPPFAALTGIRCSTAHITAKDSAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3670..3867
                     /locus_tag="ACHY09_002111"
     CDS             3670..3867
                     /locus_tag="ACHY09_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFS3885082.1"
                     /translation="MESLTTETALDILIAWLQNNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            3952..4818
                     /locus_tag="ACHY09_002112"
     CDS             3952..4818
                     /locus_tag="ACHY09_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS3885083.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMQSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHRSKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISGMQADRLADLERMLHLLSGKPIPDNR
                     GNITINLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            4890..5153
                     /locus_tag="ACHY09_002113"
     CDS             4890..5153
                     /locus_tag="ACHY09_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFS3885084.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            5150..5476
                     /locus_tag="ACHY09_002114"
     CDS             5150..5476
                     /locus_tag="ACHY09_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3885085.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(5890..6426)
                     /gene="yghD"
                     /locus_tag="ACHY09_002115"
     CDS             complement(5890..6426)
                     /gene="yghD"
                     /locus_tag="ACHY09_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFS3885086.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(6428..7606)
                     /gene="gspL"
                     /locus_tag="ACHY09_002116"
     CDS             complement(6428..7606)
                     /gene="gspL"
                     /locus_tag="ACHY09_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFS3885087.1"
                     /translation="MSSMLEIFIPLCASDPIRWQHRTTDVEHGIWSDVDNEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGPDAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(7603..8580)
                     /gene="gspK"
                     /locus_tag="ACHY09_002117"
     CDS             complement(7603..8580)
                     /gene="gspK"
                     /locus_tag="ACHY09_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFS3885088.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVAQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(8577..9182)
                     /gene="gspJ"
                     /locus_tag="ACHY09_002118"
     CDS             complement(8577..9182)
                     /gene="gspJ"
                     /locus_tag="ACHY09_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFS3885089.1"
                     /translation="MRRTCAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(9179..9550)
                     /gene="gspI"
                     /locus_tag="ACHY09_002119"
     CDS             complement(9179..9550)
                     /gene="gspI"
                     /locus_tag="ACHY09_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFS3885090.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(9547..10110)
                     /gene="gspH"
                     /locus_tag="ACHY09_002120"
     CDS             complement(9547..10110)
                     /gene="gspH"
                     /locus_tag="ACHY09_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFS3885091.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQAF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYA
                     SAQNACWAVKLAHDGVLSLNQCDEGMP"
     gene            complement(10114..10569)
                     /gene="gspG"
                     /locus_tag="ACHY09_002121"
     CDS             complement(10114..10569)
                     /gene="gspG"
                     /locus_tag="ACHY09_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFS3885092.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(10586..11809)
                     /gene="gspF"
                     /locus_tag="ACHY09_002122"
     CDS             complement(10586..11809)
                     /gene="gspF"
                     /locus_tag="ACHY09_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFS3885093.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RLNASTGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(11809..13302)
                     /gene="gspE"
                     /locus_tag="ACHY09_002123"
     CDS             complement(11809..13302)
                     /gene="gspE"
                     /locus_tag="ACHY09_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFS3885094.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTVQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(13302..15362)
                     /gene="gspD"
                     /locus_tag="ACHY09_002124"
     CDS             complement(13302..15362)
                     /gene="gspD"
                     /locus_tag="ACHY09_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFS3885095.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDDQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(15392..16351)
                     /gene="gspC"
                     /locus_tag="ACHY09_002125"
     CDS             complement(15392..16351)
                     /gene="gspC"
                     /locus_tag="ACHY09_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFS3885096.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHILSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTVVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFREGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(16369..16779)
                     /gene="gspS2"
                     /locus_tag="ACHY09_002126"
     CDS             complement(16369..16779)
                     /gene="gspS2"
                     /locus_tag="ACHY09_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFS3885097.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(16845..17654)
                     /gene="pppA"
                     /locus_tag="ACHY09_002127"
     CDS             complement(16845..17654)
                     /gene="pppA"
                     /locus_tag="ACHY09_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFS3885098.1"
                     /translation="MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLKALF"
     gene            complement(17784..22339)
                     /gene="sslE"
                     /locus_tag="ACHY09_002128"
                     /pseudo
     CDS             complement(17784..22339)
                     /gene="sslE"
                     /locus_tag="ACHY09_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
BASE COUNT         5108 a         6310 c         5668 g         5333 t
ORIGIN      
        1 atgctgcgtc tgcggcgggc cggacagata accggtcagc atgtgcctga aattattctg
       61 ctcaactccc atgacggttc atccagctac cagatgttac ccggatattt tcgtgccatc
      121 tgcaccaatg gcctggtctg cggtcagtcg ctgggagaag tccgggtgcc acaccgggga
      181 aacgtggtgg acagggtgat tgaaggggct tacgaggtgg tgggcgtttt tgaccggata
      241 gaggagaagc gtgatgccat gcagtcgctg attctgccgc caccggcacg ccaggcgctg
      301 gcacaggcgg cactgactta ccgttatggt aatgagcatc agcccgtcac caccgctgat
      361 attctgacac cgcgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc
      421 atacaggaga atatgctgaa aggcgggatt tccggccgca gtgcaaaagg aaaacgtatc
      481 cacacccgtg ccattcacag catcgacacc gatattaagc tcaaccgcgc attgtgggta
      541 atggcagaaa cgctgctgga gagcatgcgc tgataaaaca tatccctgaa ggcgtgattc
      601 aggtaccaga atcacccgac gccccggagg cgctgccggg gtgtcgttcc atttctgatg
      661 atgacctgtg cacctggtgt acccgtctgc tttaccgtcc cggagaactc agtctgtgca
      721 gactgagtac aggcaatggc atctggccat ccgtctgtga ccggaacggt tacgcgtacg
      781 gctgtccgga atttcagccg aatatcattc atccctgata ttgccgggtc tcacggtcag
      841 tgatgaacgt gatgttgtca gcccccggcc tttatttcct ctcatttatt cacattacaa
      901 ggatttcaca catgaaaaca ttgtctcaga acaccacctc ttcagcctgt gcaccggaga
      961 ctggcctgca acaactggtt gccaccatag tccctgatga acagcgcatc agcttctggc
     1021 cgcagcattt tagcctcatt ccacagtggg tgaccctgga gccccgtgtc ttcggctgga
     1081 tggaccgttt gtgcgaagac tactgcggtg gtatctggaa tctgtacacc ctgaacaacg
     1141 gcggcgcatt tatggcccct gaaccggatg acgatgatga cgaaacatgg gtgctgttca
     1201 atgtcatgaa cggtaaccgc gctgaaatga gtccggaagc cgccggcatt gcggcctgtc
     1261 tgatgacgta cagtcatcac gcctgtcgta cggagtgtta tgccatgacg gtccactatt
     1321 accggctgcg ggattacgcc ctgcagcatc cggaatgcag cgccattatg cgcatcattg
     1381 actgaatgga ggcagggaca atgcagcagc tttcctttct gcccggagag atgacgcccg
     1441 gcgagcgcag tctcattctg cgggccctga aaaccctgga ccgccatctt catgaacccg
     1501 gcgtggcctt cacctccacc cgtgcggcac gggaatggct gattctgaac atggcaggac
     1561 tggagcgtga agagttccgg gtgctgtatc tgaacaacca gaatcagttg attgccggtg
     1621 aaaccctctt cacaggcacc atcaaccgca cagaagtcca tccccgggag gtgattaaac
     1681 gcgccctgta ccacaatgcc gccgccgtgg tgctggcgca caatcacccg tccggtgaag
     1741 tcacccccag taaggcagac cgccttatca ccgaacgtct ggtacaggca ctgggcctgg
     1801 tggatatccg ggtgccggac catctgatag tcggtggcag ccaggttttc tcctttgccg
     1861 aacatggtct gctttaaccc gtcaccgcca caatcacatc acacctgttt tcacttttat
     1921 cttctgtctt cagaggtatc ccattatgaa aattatcacc cgtggtgaag ccatgcgtat
     1981 tcaccaacag catcctgcat cccgtctatt tccgttctgc accggtaagt accgctggca
     2041 cggcagtgct gaggcgtata ccggtcgtga agtgcaggat attcccggtg tgctggccgt
     2101 gtttgctgaa cgccgtaagg acagttttgg cccgtatgtc cggctgatga gcgtcactct
     2161 gaactaatgt tccgtcaccc ggaaaacgag tacgaccaga tgacctcaga cgccgctgtg
     2221 gatgaggccc ttctcatcaa tgaatacatc ttcaccgaag ccggtctcca caccggctga
     2281 cctgtcatcg ttaacccctt cttaaccgat tttctgtgag gactttatcg tgtcagacac
     2341 gcaccatgag acaaattatc ccgacgacta taacaaccgc ccctggtggg ggctgccctg
     2401 caccataaca ccctgttttg gcgcccgtct ggtgcaggag ggtaaccggt tgtattacct
     2461 tgccgaccgc gccggtatca gaggccggtt cagcgacgcg gatgcgtacc atctggacca
     2521 ggcctttccg ctgctgatga aacaactgga actcatgctc accagcggtg aactgaatcc
     2581 ccgccatcag cataccgtca cgctgtatgc cagaggactg acctgcgaag ccgacaccct
     2641 cggctcctgt ggttacgttt atatggctgt ttatccgacg cccgaaacga aaaagtaaat
     2701 catcagaata accttctgct ccggcctggt gttttcacca cgccactttc ccatttttca
     2761 tctctgcata tcaggaaaat cttcagtatg aacacattac ccgacacaca cgtacgggag
     2821 gcatcgcgct gcccatctcc agtaaccatc tggcagacac tgctcacccg actgctggac
     2881 cagcactacg gcctcacgct aaatgacaca ccgttctccg atgaacgtgt gattgagcag
     2941 catattgagg caggcatttc actgtgtgac gcggtgaact ttctcgttga aaaatacgcg
     3001 ctggtgcgta ccgaccagcc ccggttcagc actagtcccc gctctcagtt aataaacagt
     3061 attgatatcc tccgggctcg ccgggcgacc ggcctgatga cccgcgacaa ttacagaacg
     3121 gtaaataaca ttacccgggg taagcatccg gaggcgaaac gatgaaactt tccctgacgc
     3181 tggaagccga cagcattaac gtgcgggcgc tgaacatggg gcgcattgtc gttgacgtcg
     3241 atggtgttga gcttgctgaa ctgattaacg tggtctgcga taacggtcac tcccttcgtg
     3301 ttgttgatga atctgaccgg gcctcaacag actgcacgcc accatttgct gccctgaccg
     3361 gcatacgctg cagtaccgcg catatcacgg caaaagacag cgcctggctg tactcgctgt
     3421 cacatcagac gaatgacttc ggcgaatcag aatggattca ttttaccggg tccggttatc
     3481 tgttacgtac tgatgcgtgg tcgtacccgg ttctgcgact taaacgcctg ggcctgtcaa
     3541 aaacgtttcg tcgtctggtc gtaacgctca tccgccgtta tggcgtcagt ctcattcatc
     3601 tggatgccag cgctgaatgt ctgccgggtt tacccacctt caactggtaa ccgggacaaa
     3661 agacacagta tggaatcatt aaccacagaa acggcgctgg atattctgat tgcgtggcta
     3721 cagaacaata tcgactgcga atccggaatt atcttcgaca acgatgagga taaaacggat
     3781 tcggcagcac tgctgccctg cattgaacag gccagggaag atatccgtac actgcgccaa
     3841 ctgcagtttc tgcaacagaa ccggtgagtc ttactcacca gacttcattc cactgacgcc
     3901 agcctgaata cggctggcgt tttcgtttat aaaccaaaag gaaaaacgat gatgtcagat
     3961 atcacagcct cccgtccgga agtggtcacc gggcatacgg acgttatttg ctcaaccagt
     4021 atccgccaca ttctggctgt acgaaagagt acactgctgc aaatcgacac acttatccgg
     4081 caactggctg aaatctcagc aatgacagaa agtattggcg gtaaaaccgc actggactgg
     4141 gcgatgaaac aggattttcg ctgcggctgc tggctgatgg agaaaccgga aaccgcaatg
     4201 aaagccatca cacgcaatct cgatcgcgaa atctggcgtg acctgatgca acgttccggg
     4261 atgcagagtc tgatggatgc gcagacccgg gacacatggt acaggtcact ggaatatgat
     4321 aattttccgg aaatcagtga agcgaacatt ctgagcacat ttgaacaact gcaccggagt
     4381 aaggatgaag tgtttgagcg gggagtgatc aacgtcttca aagggctgag ctgggattac
     4441 aaaaccaact caccctgtaa atttggcagt aaaattatcg tcaacaatct ggtgaggtgg
     4501 gaccagtggg gatttcatct tatcagtgga atgcaggcag atcgcctggc tgacctggaa
     4561 agaatgttgc atctgctcag cggtaaaccg atccccgaca accgggggaa tatcaccatt
     4621 aatctggatg accacataca gtctgttcag ggtaaagagg actatgaaga cgagatgttc
     4681 agtattaaat actttaagaa aggctcagta cacatcacgt tcaggaaacc agaactggtt
     4741 gacaggctta atgatattat tgccagacac tacccggaag ccctggcaag cacagtgaac
     4801 aaaagccgca aggcataaaa gaggtatttt acaattcgcg aatattaact attattaatt
     4861 gatagcaatc aatcggcgag aaaatcacca tgcctaccag tgtatccctc agtccttact
     4921 ttgaaacgtt tatccgcgaa caaatagaaa gtggtcggta taacaataca agtgaagtca
     4981 ttcgtgccgg acttcgtgct ctggaagaac gggaacagca gataaagctt gaatcactcc
     5041 agagcgccgt taccgcaggc atcaacagcg gtgaaagtaa aagtgctgaa gaggtattcg
     5101 ggcgactgac gcacaaatac aaaaagatgg cagaaggtga acagccaata tgaaactgac
     5161 tgtttcacca ttagcagaac acgatcttga agctataggt gactggatcg ctcaggataa
     5221 tcctgtccgt gcaatcagtt ttaccgaaga gctttaccaa caatgtctgt tgatagccga
     5281 atcgccagta ctctacagag aaagacccga attaggggct gggatcagag gttgttcgta
     5341 tggtcgttac cttctgctgt tcagggtact ggatactgaa gttcgtatcg aacgtatcgt
     5401 tcatggttcc cgggatatca acagggtact tgcagagcca gacgacaaac catcagatca
     5461 tttctggcag ccataaacaa aatctgtgcg ttaacgctcc ctaacacagg tagccaggta
     5521 ttttataaat ctctgtattt tgttgttact tcgattccgc aattcagcat atcgaaccag
     5581 acaaaatgat tacagcaaag cacaaaaaag agggattata gcgtcaaaag aattcgaatt
     5641 gcacatgggg gcctcattgg tggcctcaaa aacaacaaag acaaaacaat cgatacaaaa
     5701 caattggtta tataaccagt ttcattccga gtccgggcac cactattcta aagaacccgc
     5761 tcacaaggcg ggtttttgct tttggatctg acaataacct tcacgaaaca aattagctta
     5821 taaagcctgg gggaattact ctcgccacgt taacgagagt aattttattg atattaatct
     5881 cctgatactt tatccccgtc caaactccag ccgctgcaca ttcaccatcc cagtcttctc
     5941 agcagcactg acatcaattt gtgtcacccg cagcgcatat ttttcatcca gtgcgtttaa
     6001 ccatttcagc aggtcattaa acaccacagg ttctatccag acctgaatat tctccccacg
     6061 ctcggctatc cgcttgatga ccaccgagtg cgcggaagca ctgtcactga tgacccgcga
     6121 tacctgcgca ggcgttgttg tggcagattt tcgcgctgca ataatatccg gcgcggcgtt
     6181 cttcagtcgg gtgttcattg ccaccagctg ctgcaacatc gtctcctgtt gttctatccg
     6241 ttcgctcaac ggctgccaga tgaggacgta atatccgacg ctaaacagga acaccaccgc
     6301 tgccagtaac atgccctttt cacgcggaga acgccccgcc agatgttgtg tcagccagtg
     6361 ttcgccacgg cttaactggc gttcacgcca ttgctgaaaa tagtgaataa atttatcgcg
     6421 taacatgtta tttcctccgc aacgttacgc cgccggaaac cgcatcaccc tctttctgta
     6481 acgcgtcctg ttgcacaaca taatctgccg ccagtgcgct acgcagtttg tcgaagctgg
     6541 caaagttcgc agcccgtagc tggaggtgaa gcgtctggcg tttttgatca aaggtgaaac
     6601 cacgcatttc gatgtcggta agtgacgctg atttcagggt actggcgatc gctgacaatt
     6661 ctgcgagcag ccgggtatcg tcggcctgcg ggcgatattt tttcagcgcc atcgtcacct
     6721 gagagcgtaa attcacaatc cgcttctgct ccgggaacag tgttaagaac tgtttctccg
     6781 cctgggtgcg gctttgcgcc acctgttcgc tgacgctcca taacgtcacg ccccgttcca
     6841 ctgccagcgc aaccagaatc agcaatatcg gcagaatcat cacccgccag cgcgcccact
     6901 gttttcggta gctgacgcgg ggctgccacg gccctgttag caggttccct tccggttcgc
     6961 cataagtggt aatggcgggc agagccgtaa cggtcaggcg ttcagcatct gggccagccc
     7021 atgctgatag ctcttccggt gcaatgccga ccacggttag tgaaagcggt aaatcctgct
     7081 cattgagctg tgcgcggaac atgaccggag ccagcgccag cccggcgctc catccccggc
     7141 attcatcgat gcggcagata acccgttgcg catcgccagc cataaaccca caaggaatgg
     7201 acatccagtc tggcgcgacg gtagcgcggg tgatgccgtt tgcctgcaac cactgcgcaa
     7261 tgttgcgcat atgctgttgg tgaatcacag ctacggttgc cagttgctgg tcgattttca
     7321 acggggcgaa atgcagttca tcgatatcct ggttcagctc ttcttccagt aaggcgggca
     7381 gaatagtcgg tatctgcttg cgggcgacat caggcagttc aacctgccag acgctgatcc
     7441 attcgccggg aatgtagagt cgaatcgcat cagtttgcag ccattgctgg agctgttcgt
     7501 tgtcaacgtc agaccagatg ccgtgctcca cgtcggtggt acggtgctgc caacggatgg
     7561 gatcggaggc gcaaagcggg ataaaaatct caagcatgga actcactcac tttctcctgt
     7621 ctgatgccag agaaccgaaa agtgttgtgg gcccatgcgg acaattaacg agttcatcgt
     7681 cagttcaatc tcattcacgg tgatatctga acgcagccag aagtaattgc tgtccacgct
     7741 caggacggtt tttagctgtt ttttagtacg ctcatcgacg tcagcaagta gcggctgtgc
     7801 aagaaactga tcgacatctt cccagccctt cgccggacgt tgttgtaata acgcccgcgc
     7861 ctgaacaggg cttaaccacg ggtcaaacag cgcctcaaga atcacacttt gcgcgacgtc
     7921 cagggtatta atgttgattt gctggcgggt catcggcagc gcacagacca gcggtttcag
     7981 tttttgataa agtccggcgt ccattccctg caccacgcgc atctcgctga tatcagccag
     8041 cggttgatta gcggcgtaaa acggcaccga acgggcgaga tactcgctgt cttcacggcc
     8101 cagacgcgtc tgcacgctgc ggtcttcgtc aataaactcc cacaggcttt cggctatcag
     8161 ttcggcccga taagcaggca catccaggcg cgtgatcagg gcaatcagtt gttgtaccgc
     8221 gagcggacgc gacgccgtcg tcggctgagc gagagcattc aggttaaagc aggcctgtgc
     8281 gtcacgcaga gtgacggcga tttgcccctg cggcagtggg aaaaaacgag ggccggaagc
     8341 ccacggctgc gccagatgca cgcgcttttc atttttcagg ctcagactga gtgcgctcaa
     8401 cgccaggctt tccgcactgg cgctgtacca cagcgcctgc tggtactcct gctggctgcg
     8461 cgttcgccca agttgttgct gcattcgccc ggaaagcgtg atggtcacca gcatcataac
     8521 cgccagcaat accagcacca cgaccagtgc cattccgcgt tttggtggtg aggtgatcat
     8581 gataattgcg gcccgcgtaa caaccagatg cgttcaatct cgccccattg cggcgaatgc
     8641 agagtcatgc gcaccgccac tggtatcgcc tgcactgatg accagctctc ctgccagcgc
     8701 gtgccgtcgt agaactgcaa acggagcgaa tccgccggga ttaatttttg cgttgttggc
     8761 ttcacgctgc ctgccgcatc ggtcagtggc caggctaacc gttcgagata accaccatga
     8821 atgcggtaac cgacggtgag cagattactg cgcggcagac gcatcaacgg attaaccacg
     8881 ccgccgcgca caaaacgcat cccttcactc tcagacgcca gcacgccagc gcccgccagt
     8941 aacgctggtt cacgctggcc ctgatcgcct cttaccggac gcggcatcat ttgtgtcaga
     9001 tcgtggttca aaaaactcat cgtttgctgc atgaggttta gtttttgatc gtgtccggcg
     9061 acggcgctat tcacgcgtgt aacgccgttt gtcacctgct gcgccatcag tgccagtgag
     9121 gcaaaaatgg caattgccac cagcatttcc agtaacgtga aaccagcgca agtccttctc
     9181 actgttggcc tcccacggcg ctaaaccagg cgcgtcgtga ctgaatcacc gacgaaaagt
     9241 cttcgtgacg gctgacttca atatccactg cctggagcag cgcgttgcct gtactcagcg
     9301 gtgtggttcg ccagtaccag cggcttcctg ccatatcact ctcgccctgg tgcctgaccg
     9361 ctcgttgttc acgggtcatc aggtaaagca gtgcggtctg gttttcagct acccagcccg
     9421 ctaccgtttt ttcttcaagg atttgctgat tactcagcgc gccgctggcg atctgtaaca
     9481 ccgccatggc ggccaacgca aaaatcgcca gcgcgagcat tacttccagc aaggtaaatc
     9541 cacgcttcat ggcatcccct catcacattg attgagggat aacacgccat cgtgcgccag
     9601 ctttaccgcc caacacgcgt tttgtgccga cgcgtaaaaa cgcagtgtaa acggcgtggc
     9661 gggttcaaaa ggcgaaaaac ggatctgcgg tgtcttcttt ttcgcctctt tttgcagctc
     9721 cagttcaata tcgtgcagcg tcaggcgacg acgctgcagc tccagcgcat attccttctg
     9781 ccagatatca cttccgggtt gtaacagcat ctgcacctgt ttcggcaccg taacctgtgc
     9841 cgataaccgg gtcgcagaaa cgggcaacca ctgcccgtga cgacgctgca taaactggta
     9901 acctggtggg tcgattagca caccgagtgt tttcccttcg atcactgccc tgtccttaaa
     9961 ctgcgcaaag cgagtcagaa aagcctgcgc cgcttttttc gcaggcggct ctgaagcggt
    10021 cgcaaacgtc tgtaccacgc ccgcactggc aaggccgata aggaaaatca ccagcatgat
    10081 ttccagaagt gtgaatccgc gttcaggcac tgattactga aactcctgca aattccagtt
    10141 accgatatct gcgccagcgc cctccccgtt ttcctgacca tctgccccca gggtatacac
    10201 atcaaacagt cctttttcac ccgggctgag atactgataa tcattgcccc acggatcttt
    10261 tggcagtcgt ttaatgtatc caccggtacg gtagttgcgg gagtccgcca tattggccgg
    10321 ttgctggatc agcgcctcca gtccctgttc ggtagtcgga taacgcccgt tatccagtcg
    10381 gtacatatcc agcgcattct ccagcgccac gatatcgctg atggcttttt gccgatcggc
    10441 tttctctttg ttgcccagca ggttaggcac caccagactt gccaggacgc caagaataac
    10501 aatcaccacc atcacttcca gcagggtaaa acctgcccgt ggtttttgtg tgcgggataa
    10561 cgaattcatg tgataactcc gtaaattaca ttccaaccat attgttcagt tgcagcatcg
    10621 gttcgaggat ggcgatgacg ataaacagca ccacgcccgc catcatcacc accagcgccg
    10681 gctcaaacag ccctaacgcc agccccacct gggtatcaaa ttctcgttcc tggttaactg
    10741 cggcctgttc aagcatggtt tccagttcac cgctctgttc gccggaggcg atcatgtaca
    10801 gcatcatcgg tgggaacagg cgcaactccg ccagcgcagc gcgcagactg cttccttcgc
    10861 ggacgcgatc tgccgccagc agcagttgtt gttcgacata gcgatttgcc gacacggcgg
    10921 cagcggtctg aatgccttcc agtagcggaa cactgctggc ggtgaggatg cttaacgtgc
    10981 gggaaaaacg cgccgtattc agcccgcgcg caacacgccc cgtcacgggc aaacgcagca
    11041 aggttttatc ccagcgtagg cgcatggcag gatttttgag tagccgttgc cccagcacca
    11101 gaagccccag caatcccgcc agccagtaaa cgccgctggt ctgtaacgtg tcgctcatgg
    11161 cgatgagcgt gcgggtggag gcaggcagcg cgtgtccgag atgatcaaac tgttcgatga
    11221 ttttcggcac cactgccgtc agcaaaatag tgactacgcc cgttgccacc accagcaata
    11281 ccagcggata gagcatggcc tgcaacagtc gtgatttcag gcgctgtcgc tgttcggtgt
    11341 aatccgcaag gcgattgagc accacgtcga gatgcccgga tttttctccg gcagcgacca
    11401 tcgaacaaaa cagggaatca aagacgcggg gatgttcgcg caggctgtcc gacagggtat
    11461 aaccttcctg aatccggctg cgcagcgcca ttccgaggct tttcacatgc agtttttcac
    11521 tttgctcact gaccgcctgt aagcaggttt ccagcggcat tgctgcctgc accagcgttg
    11581 ccagttggcg cgtgaacagc gccaaatctg ccgccgccac gcgacgatgt gcgtgccgcc
    11641 gacgctgcaa cattccccct gtcgaagcat tcagccgggc ttcaatgtgc acggggataa
    11701 gctctttacc acgcaacaac tggcgggcat gacgcgcgga atccgcctca atcattcctt
    11761 tggttttgcg accattacgc tccagcgcct gatagtaaaa cagtgccatt acgcctccat
    11821 agttacccgc agaacttcat cgagagaggt ttctccggcg agcactttct caatgccgtt
    11881 gctgcggatg cccgcagagt gttgccggac ataacgttcc agctccagtt ccccggcctg
    11941 acggtggatc aaatcacgca atgtggcgtc caccacgatc aactcatgga tggcagtacg
    12001 cccgcggaaa cctttgtgat tacaggcggg gcagccctgc ggatggtaca gcgtgacagt
    12061 acgggcgtcg gtaatcccca gcaggcgttt ttcttcgtca gtggcaggcg cgggctggcg
    12121 gcagtcgggg cacagcgtgc ggaccagtcg ctgcgccatc acgcccgtca ggctggaaga
    12181 gagcaggaaa ggctcaacgc ccatatcctg cagacgtgtg atcgccccca ccgccgtgtt
    12241 ggtatgcagc gtggaaagta ccaggtgccc cgtgagcgac gcctgaacag cgatttctgc
    12301 ggtttctgta tcgcggattt caccgaccat caccacatcc gggtcctgac gcaaaatcgc
    12361 gcgcaggcca cgggcaaagg tcatgccgac gcgggtgttg acctgcgtct gaccgatccc
    12421 ttcaatcatg tattcgatgg ggtcttcaac cgtgagaatg ttgcgcgaat ggttgttcag
    12481 ctcctgcaat ccggcgtaca gcgtggtgct tttgccggaa cccgtcggtc ccgtcaccag
    12541 aaagatgccg tgcggtttgt gtaacagctg gcgcaactgc acggtcagtt gctgactcaa
    12601 ccccagacgt tccagcgtca ggcgggcctg gtttttgtcc agcagtcgca gcaccacgcg
    12661 ctcgccccag gcggagggca tggtggagac acgcacgtca atcgcccgac cgcccagcag
    12721 cagcgcaata cgcccatcct gcggtacgcg cttttcggca atatccagcc gcgccatcac
    12781 cttgatacgc gataccagca gcgaggccag tttgcgcccc gggcgcagca tttcatgtaa
    12841 tgtgccgtca acacgaaaac ggatcaccag actcttttca aatgtctcga tgtggatatc
    12901 cgaagcgcct tctttaatcg cctctgccag catggcgttg atcagtttga tgattggcgc
    12961 gtcgtcgtcg ctttccagca gatcttccgt ttccggcagt tcttcggcga gggtaaaaaa
    13021 atcctcagca gaaccgagat cttccatcag ctggcgggcc tcggaagagt cccgctgcca
    13081 gaccgcattc agccgctgtt caaattcggc ttcgtcgatt tgccgcagcg taaagggcgc
    13141 gttcagcccc cgctgcagct cctgcaaaac agagagcgac aacggatgaa cgtggaggat
    13201 ctccagcgac gcttcgcacc atgccaccag gctaaaccga cggctgaaac tgtagggcag
    13261 acgcacggtg ttagcggtgg tttcctgtgc tacaggcacc attaacgcgt tctcccggca
    13321 ttgaggaatg cgcgaacttc cggcggtaag gcctggtttt gcgctggcag taccggctgc
    13381 gcggtgtgcg gcatcaggct taagccttga tcatcgcgat agatctgctc ggcgcgcatg
    13441 tagttatatt tgcgctgcga cacgccgtct gccgccatac cgtcacgcag aatggtcggg
    13501 cggataaaca ccatcaggtt acgtttttct tttttatccg ccgtcgattt aaacaggtta
    13561 ccaatcaacg ggatatcgcc cagcagcggc actttcgcca cgctttctcc cgcctggtcg
    13621 tccatcagac cgccaagcac gatcagttca ccatcgttag ccagcacggt ggtcttcagt
    13681 ttgcgctcac caaacaccac gtcgaggctg gtctgtcctt ccaccttcga cacttcctgc
    13741 tcaatcacca tctgtaccgc gtttccttcg ttaatctgcg gcgtgacttt cagcatgatg
    13801 ccgacttttt tcctctctac cgtattgaaa ggattgctgt tattggagcc aacggtagat
    13861 ccagttaata ccggaacgtc ctggcccacc atgaagaagg cttcctggtt gtccagcgtg
    13921 gtgatgctcg gcgtggagag cacgttcgag ctggagtcgt ttttgaccgc ctgtaccagt
    13981 gccatccagt cgcctttcac cacgccaacc gccgtaccgc taaagccaga aagcagctgg
    14041 gcgagcgtgg agagatcgcc gttggtatcc gggttaatgg tggtggcacc gttttcactg
    14101 atcaccgtgg agcctttctg cggtttcgcc tgagaaatgg ctgcccccag cgtgccgata
    14161 gggatctgcg taccgttagc aaactgcatt aatccggcat ctttcgacgc ccactgcacg
    14221 ccgaaattga tattgctgcc ttcagcaact tccacgatca acgcctcgac gtgcacctga
    14281 gcacggcgaa tatccagttg ttcaatcacg ctttgcagcg actgcatgat gtcctgcggc
    14341 gcagtaacaa tcagggcatt actgtgtttg ctggcggcga tggagacaac ctcacgcccg
    14401 ctaccgacgg tgccttccgc ctcttcttta gccgccgtga gcgtgccgct gacctgcttc
    14461 agcacatcga ccagatcttc cgctttgctg tatttgagat agaaaacctg gctgttgccg
    14521 ctgcgctcca tttctgagtc cagccgacgg atcaggcggc gcattttgtc ccgcgtggcc
    14581 gggtcaccac tgacaatcac actgttggtg cgttcgtcgg cgacaatttg agatttcagc
    14641 gtcgcaggtt ggttctcgcc gctgtttttc gtcaggcttt ccagcacgcg ggcgatttcc
    14701 gaagccgagg cgttatccag cgggatcacc tcttcagtgc gattacccgc gtgatccaca
    14761 cgctggatca cttccgtcag ccgctccacg acggaggcgc gcccggtgag cataatcacg
    14821 ttggagggat cgtaattaac aacgttgcct gagcctgcgc tgtcaatcat ctggcgcaga
    14881 atcggtgcca gctcgcgtac cgacacattg cgtaccggca cgactttggt aaccatttca
    14941 tcgcccgcgt agttgtcgct gccttcaccg accagcggca gcggctcgac tttcgcggcg
    15001 cttgatttca ccaccttcag cacgtcgttt tccatcggta cgaccgcata cccctgtgct
    15061 tccagcaggt taaggaataa ctggtaatac tggcgttcat tgagcggagt catagtgcga
    15121 atactcactt tcccctgaac gcccggcccc ataatgatgg ttttattaag gttagcgccg
    15181 acggtttcga tgaacgattt caggtcagta tctttaaaat tagcggtgaa agtggcttct
    15241 tccgcccaga ccggtgaact acacaatgcc gctgccagca tcagcggcag taaacgcttt
    15301 tttgttttga ggccagttgt cttcttacgc cagatagaca acgtcatatc acgccaaaac
    15361 acgatgaatg attctcctgg attaaatgcg gttagcgcag tgcgatggaa atgtcgtagc
    15421 gcgcaccctt gcgtaaaacc gtaagttgaa tggaatccat tgaaggtaac tgccgcatca
    15481 gagcaatcat tgctcgtgga tcagtgaaat cctgctgatt tagtgcaatg gcgatatcgc
    15541 cttccctgaa accgctggca tcgaacagag aacgatctgc ccccggcttc actgcataac
    15601 cgacaattcc ctccttacgc acaggcgtaa gctggatata gttaaaaatt ttctgcggat
    15661 ctttcgccag tgcctgacgc acggcagccg ggatctcaac tggcgcactg gcagcaggct
    15721 cagcaacagc ttgctttgct tcgtcgctga cggctttttt gctggtgacc gccacagggg
    15781 ggcgttcctc ctctgccaga ctcagacgtt ccattttccc ctgatagcgc agcatcacat
    15841 ggtcgcggtt gatttcctca atcactgcgt tgtgagagcc aagccgttca ccctgcaaat
    15901 agacctgctg tttgccgcct tcttcaatca ccgcgccggg tctggcacca aaggcgatcc
    15961 cacgcagcac cacattaaga cgcgtttctg ccacaggcgc agattcaggt tgttttaccg
    16021 gtgcggcgac gggttgatat ttgccaaacc agttttgttg gctgactaat tgcacgtcat
    16081 ttttatcgaa cggttttgca tccgcacgga gcggtttatt cgccgatgag gaaaccaccg
    16141 tatattccgc agaaaaggag aaatagcgcc agagtgaata cgccattttt gcagaaataa
    16201 taagcagcat gagccagaac atcccgcgca ccatttttcg cagatgctct ttgtctgtat
    16261 taagagattg tctctggcta agaatgtgac gaattttagt cagccattga atgagataaa
    16321 ttcgtgcgtc acgaaaaaca acccgcgcca atttatatct acccgacgtt atgctttgac
    16381 tattccacag gtggtacgat ccagtttccg ctgatactgg ttacctgtac gtgtatcatt
    16441 aatcgttatg ctgtaattaa ttccctcggt gatcattaat ttcaccgttg ggtcagcgca
    16501 catttgtcgt tgatagctgg ttaaaaaggc gtcaggtgtc tgcgtggtct gagtacccga
    16561 ttcgctgata atggtcattt ttaccgtcgt gccactactt tgcgccagca ctaaggtata
    16621 tcccgcagat ttaatcggca ggttttggct gatattttgc gcctgttttt tcgccagtaa
    16681 actggcgttt tcattatggc tggcgcagcc acttaataat cctgtaacgc tcaacaatag
    16741 cgatattaat accctccctg gcatttgttt tatcgacatg gtaaataatc tctaaggtta
    16801 ttaataagag ttaaaatgtc actttgataa tgacgttgtt atcattaaaa caatgccttt
    16861 agataaagtg ttgctatacc gcccagactt aaacacggtc caaaaggcag tgtggttgat
    16921 cctctttttg taataacggc atatatcagg ccgcagcatg aggcgattaa agcaacgttg
    16981 ggcagtgata acgcccccac ccagccacct aaagcggcga agagtaatac atcgcccatg
    17041 cctaatgctt ctttacgcag aactattccg gctatccagc gcagggagta aaaagtgata
    17101 aatcccacca gtacgccggt gactgcgtct tgtagcgtga gcggactctg ctgcgcccat
    17161 gccgcaatta gtcccgtcca caatacgccc tgagtaaaaa catcgggcag ccattggtta
    17221 tcgaggtcaa tgacgctcgc ggcaatcagc caggcggata atatcatcac cgccagcgcc
    17281 catccacttt ccggccacac caggctcgcc agcaaaaaag cgagtgccgt caataactcc
    17341 accagcggat aacgcttgct gattttcgcc tgacagtcgc ggcagcgccc tttgagcatc
    17401 aaccaggaga gcagcggaat attgtcacga acgcggatag tctgctggca atgcggacag
    17461 tgcgagcgtg gcagcgccag gcttattttt gactgcgtac tcggcatttc accgtgaaac
    17521 tccgccattt gttggcgcag catgatgggg taacgccaaa tcaccacatt caaaaaactg
    17581 ccgataatca atcctccgac ggtagccagg acgggcatcg ccgcggggta ttgctgaaaa
    17641 acatcaaaaa acatggttaa aggttatttg ttgtaacttg ccggatgcgg gcctgcaggt
    17701 gtatgccata cggctttcct ccaggcccga tgcgccttat ttcatgccgg atgcggcgcg
    17761 agcgccttat ccggtataca ggcttactca gctgacatct tatgctcggt aacctgatta
    17821 atggtttccg gcccttgctg cggtttcggc agattaagcg ccgccagcgt ctcgtaagcc
    17881 gactggctca caccgccctc gaagttcatc tcgctcgctc ccggcaactg gtaagcattc
    17941 gcgcccggat tccatttctt aaagaactcc gaaagatccg tctgggcgac ccaggaggca
    18001 cacagcatca gcgtgtccgc tgcgttaccg ttggattcag cacagtagtt cttactgccg
    18061 aacttgttat tgccaacctc atcgccgcgc gctttacggt gcatcaactg gaacaggttc
    18121 cagcctttca tcccttcacg ctcgctgtaa aacttaggca ggtcaccttc tgggtaccac
    18181 tgtttgatat caaagttttt ctctgcccac tctttcagct gtgcgtacat cagcagacgg
    18241 tcccccgcac cgccgcgcgc ccaggcctga ccgttgctct cgtccagata ttccggcgcg
    18301 acggtaatgt cgtcagcgac acggttcatc ttgccgagat agcgatcctg catgtacagc
    18361 gccagcacgt tgttcgccac ttcagttgca cccggtacgt tcagcggtgt ttctgcagcg
    18421 ttatgaccgg cttcgtgcca aatcagccag tcgttcagcg gcgttgtcgg cagcgtggtg
    18481 ctgttcgtcg agaagctgct gttcattacc ggataacccg agtgcgcatc accgatggag
    18541 atctgcacat cgttggcgaa acggtgttta tgccccgtca atgcttcata ggtaaacatc
    18601 cggtggttac cgctcgtttc attgcgaccg tagaagtcat tcatcgagct ggcaaaggta
    18661 tccagatctt tagcgaattc tgctacacca ccagtgaaat tgctggcctc aaggttcttc
    18721 ttcggcgtgg tatagacgaa cgacgcagac tccagctcgc ccagcggcgc cggtgagtcc
    18781 agatcgtttt tccattcgcc gtctttatag aacggcgctt ttactacacc ggtaaaggtg
    18841 aagttagcag aaacatcatc cttactgtcg cccttgatat aaatcagacc accataaggc
    18901 accttgaagg tcacttcacc gttagcctcc agagtatacg ttttagtcac tcttggcgga
    18961 cggttcagcg caacttcatg cttctcacgt ccagtcaggt cgtcagccag cgccacggta
    19021 acagtcactg ggactgacgc cgaagactta atggtgacgt cctgctgggc cggtgcccac
    19081 aggccggttg actgcatgtt acccgcaaac catttggtcg gattcgagta caggctgatg
    19141 ttttccgtaa cgctctcacc ctttgccgat acggatcctg ggtacttctc cacatcaacc
    19201 ttaatgttca gatcccacca ggaacggccc agcatcagac gcgtcagcgg tttttccata
    19261 tagttgagcg gatagcttgg gttcatcatg cccgctttgc tgctaccgtc accgtagatc
    19321 atgttgttat cgaccagcga ttttttcaga tctgcggagc acttggtgcc gcctgcatag
    19381 gcatcattgg cgtagcagtt caggaactcg gtgaacgttt taaagcccag ctcatcttcc
    19441 ttgccctctt cgtaacgata tttcgtatcg ttccacagcc agaccgacat gttctggtac
    19501 agacgttcca gatcaacact gttcagacgc tcacctttac tgcctttggt acggaaataa
    19561 agctcatgct gatacaggcg ctgaatgttg gtgcctaaat ccgccgcctg caccatcgct
    19621 ttcgcggtgt cggcgtcaag attcagttgc gtatagcgcg gaacatacat gccacctgtt
    19681 accggaacat ccgtgcctgg gcggcgctcc aaacagttaa tctcgtaatg gtaagtcgag
    19741 tccttacact cctgtaaccc aggaaacttc tcaaagattt ttgcctttgc cgcttccaga
    19801 gattcttctg ttgtgtattc cgcttcatca ataaaggcat aacgcgttac ctgtttgccc
    19861 tcaacttcct cctgccagct cgcaacttcc agtttcggct tgtcatcagg cttgttgtct
    19921 tgctggtatt tccaggtcac ttcccctgta tttgggtcga tggtgtacgg cggttgcgcg
    19981 ccgtctgcag caggataacg ttcataaacc caaatgccag tcgcgcgacg ctgacgaacg
    20041 cgatccggat acccttgcgg atcgttgttc accaccgatt tgttcagagc cattgacaga
    20101 cccgcggcat ccagcagacg cacaaaactg gacgcgctct cttccttaag attgctcatc
    20161 acgttttcca tgatcagcac cgagccacct ttgttcagat aagcgatcag atcggtcaca
    20221 tcctgctgag tcagcttcgg tttgctggta tctgcacgca gaggcacagc atagggatcg
    20281 ccagaccact gagtcacata ttcaaagccg ttgaggatca gcaacggaat ctcttccgga
    20341 ttcagatcgc catagctggt caactgttta accgtgatac cagtgaaatc ctcatggaaa
    20401 gcaaatggtg cactatttcc caatacctgg cccgcttttt tgaaataaac gttctccagg
    20461 ttggtgccga cagtcatgat gctcttggta tttggctgcc agatgtcatt tgacaagtag
    20521 cgcaggacgt tctgcataaa gtgcttcatg tcatcagaat caccgctgag cgtacactca
    20581 cctttactat taacaccacc gccccaactg taaccgttcg ggcaacgcag gatgctgttg
    20641 tagtgcgggt taccgataac catcagtttg ccttcaccga cttgccccag cgaaataaac
    20701 ggcaggttga aagtcgcagt atcgcgcgta acgttctctg gctgcacaat ggaaggcgct
    20761 tccgtaatgt acgccagctc atttttatcc caggcgcgtt tttcgccaaa cgccagccag
    20821 tagtttttat cattacgcgc catcagaatc gggaatgccg agttggagat atttaccacc
    20881 gcctgaccgc gcgcgttacc ggtgctgcca tagaagttgg tagagtcatg gaagacgtgg
    20941 aacttgctga cagactgata gttcgtatcc acgccccaca gcttgttaat aacgccctga
    21001 atctggccgt cattaacatt gcgcgttgtc agcgagaacc agcgagcctc gttacaaccg
    21061 tcggttttcg cacaaatcgc ggtatcgatc tctttggcct gacccgtctt aaactgctcg
    21121 ataaattcgt taggcagcac aacgttttga tcgccttcat ccagcgtcgc accgttggat
    21181 aacgaaagat tgattatctc gttgatcacg ttgggatatt cggcaaagac cttgcgtaca
    21241 tagtccggaa caacacgagt attattttga ccagtcgtcg aataacgatg aatgagctga
    21301 tcgatatttg ccccgcgaac ttcatcaccc aattcagtca gcgcaatggt cgacttattg
    21361 ccacgtactg agcccagttc aaaggtatcg ataccaaagg agatgtttcg ccccagctaa
    21421 aggagaattt accgttttcg tcagttacgc cacggcctga attggtgtag tagtcaacgc
    21481 cagcaacacc gttccccagg ctatccacca gttggccttc ggaaagaatg atttcagtgg
    21541 gttgatactg ataaaactgt tccgcgttag ccgacacgaa ggacgcgttc agatccggtt
    21601 ttgttccctc tgtcgtgact ggcactaccg ttgaggtatg ggtggacggc gctttatcag
    21661 tcgcagcatt gttttccacc tcttcattga ccagcttgct gaaattgtct gttgccagat
    21721 caatttgctt atacagtttt tcaaatcgcg cgcgatcaac cactgacgag aaagtaagac
    21781 aaagctgttc tgcatctgcg gggcaactgt cgctggacgt caccagagag atggcgttgg
    21841 ttttcttatt ttcagaattc gccagctcct gcgcgtcctc caggctaaac gacactttgt
    21901 caaccgcacg caggctacgc gcagcttctg actgggtgtt gaatgttgca atggtcgtac
    21961 tgcccaccac acaactcacg gtattgcctg gcgtaaaggt aaagccatcg ctggattcac
    22021 cattacaggt agcaccagtt acccgctggc ttccgcccag ggtcagataa cccgttttcg
    22081 taggaacagg ttctggctcc ggttctggct ctggatcagg tgtcggttcc ggcgtaggtt
    22141 ctgggtccgg cgttggctca ggcgtcggct ccgggtttgg tgttggatca ggtttcactt
    22201 ccggcaaaga ccctgttcca gaatctacag gcggcgtatc ggaggaagat ccggaaccac
    22261 cgccatcaca accggctaac agggttgcgc tcaaaatagc cgctaaaagc gatttcttat
    22321 atttaaattt cttattcatt aataacgcaa gtgacaaaac aatgttaaaa aattcgtaac
    22381 tgggagaaat agttttatgc tttatcttct ctaataact
//
LOCUS       DAZUCC010000063        22197 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZUCC010000063 DAZUCC010000000
VERSION     DAZUCC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.063"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..459)
                     /locus_tag="ACHY09_002129"
     CDS             complement(<1..459)
                     /locus_tag="ACHY09_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS3885099.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDVEYIFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGF"
     gene            complement(717..1970)
                     /gene="amiC"
                     /locus_tag="ACHY09_002130"
     CDS             complement(717..1970)
                     /gene="amiC"
                     /locus_tag="ACHY09_002130"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFS3885100.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2202..3533
                     /gene="argA"
                     /locus_tag="ACHY09_002131"
     CDS             2202..3533
                     /gene="argA"
                     /locus_tag="ACHY09_002131"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFS3885101.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3771..5597)
                     /gene="recD"
                     /locus_tag="ACHY09_002132"
     CDS             complement(3771..5597)
                     /gene="recD"
                     /locus_tag="ACHY09_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFS3885102.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPSGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPMMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5597..9139)
                     /gene="recB"
                     /locus_tag="ACHY09_002133"
     CDS             complement(5597..9139)
                     /gene="recB"
                     /locus_tag="ACHY09_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFS3885103.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKLLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALMLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVDEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDSLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGITLEEA"
     gene            complement(9132..12020)
                     /gene="ptrA"
                     /locus_tag="ACHY09_002134"
     CDS             complement(9132..12020)
                     /gene="ptrA"
                     /locus_tag="ACHY09_002134"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFS3885104.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12196..15564)
                     /gene="recC"
                     /locus_tag="ACHY09_002135"
     CDS             complement(12196..15564)
                     /gene="recC"
                     /locus_tag="ACHY09_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFS3885105.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWEIGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDADRLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIARRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15577..15900)
                     /gene="ppdC"
                     /locus_tag="ACHY09_002136"
     CDS             complement(15577..15900)
                     /gene="ppdC"
                     /locus_tag="ACHY09_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFS3885106.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15885..16292)
                     /gene="ygdB"
                     /locus_tag="ACHY09_002137"
     CDS             complement(15885..16292)
                     /gene="ygdB"
                     /locus_tag="ACHY09_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFS3885107.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16289..16852)
                     /gene="ppdB"
                     /locus_tag="ACHY09_002138"
     CDS             complement(16289..16852)
                     /gene="ppdB"
                     /locus_tag="ACHY09_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFS3885108.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16843..17313)
                     /gene="ppdA"
                     /locus_tag="ACHY09_002139"
     CDS             complement(16843..17313)
                     /gene="ppdA"
                     /locus_tag="ACHY09_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFS3885109.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17498..18292)
                     /gene="thyA"
                     /locus_tag="ACHY09_002140"
     CDS             complement(17498..18292)
                     /gene="thyA"
                     /locus_tag="ACHY09_002140"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS3885110.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18299..19174)
                     /gene="lgt"
                     /locus_tag="ACHY09_002141"
     CDS             complement(18299..19174)
                     /gene="lgt"
                     /locus_tag="ACHY09_002141"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS3885111.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19325..21571)
                     /gene="ptsP"
                     /locus_tag="ACHY09_002142"
     CDS             complement(19325..21571)
                     /gene="ptsP"
                     /locus_tag="ACHY09_002142"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS3885112.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21584..22114)
                     /gene="rppH"
                     /locus_tag="ACHY09_002143"
     CDS             complement(21584..22114)
                     /gene="rppH"
                     /locus_tag="ACHY09_002143"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFS3885113.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT         5357 a         6123 c         5502 g         5215 t
ORIGIN      
        1 aaaaccaatc tcatcatcag gaagttgaac gctgaattgc tcattaatga tagttaatgc
       61 atcgcgactt atcgcatact ctgctttgta caacgctttg atttcatcaa ggaaaacatt
      121 attaatttgt atacctgaaa ctactcggga tattgcattc gaaatgtgat ccgcaagaat
      181 caatagtagc gatggattga gatttttttc gagatttttc tccgcatatt gcactatttt
      241 ttcggcaaca aaaatatact caacatccac atgttcaatc aatttgtaga gtttattttt
      301 ttgttcgttt ctgacataaa aaatccggtt agccggatgt tcagggaccg acattccata
      361 ttttttgttg taaccgacgc caggtccaga aatgataacc tcctgtccat tcatcgatgc
      421 ctgtacacaa ttattgttca tgactttttc gatgatcatt ccatgactcc aaaaaaaaag
      481 gcaaggcacg tcagaagtaa taattacttc aaacattgcc ttgcctgatt taacagttac
      541 aagatgatta actaatgcgt atacattaca cggaattgtg cttaattaaa gagatggtcg
      601 gcttattttt caagccgaca ttcaaatatt cgacatcagt cacaaaacca gcatctaata
      661 ttcatgaaaa tgttatctat tcaacgaaat ataaattcat tgaataaata aaaacatcat
      721 ccccttctcg ccagcgtcgc cccatcggca aaatacgctt taatccccgc aagaatagac
      781 tccgcaactt cctgctggaa agtcgccgtt ttcagtttac gttcttcctc aacgttactg
      841 ataaacgccg tttcgaccag aatggaggga atatctggtg cctttagtac ggcaaacccg
      901 gcctgttcaa cttgattttt atgcagcttg ttgattttac cgagcttatt cagcaccgct
      961 ttaccaaact tcaggctgtc ggcaatggtc agcgactgca ccatatcgaa catggtgtgg
     1021 tcgacatagc ggtcaccgct tttgctcacg ccaccaatca agtccgaggc gttctgggtt
     1081 tgtgccagat attttgccgc agtactggtt gcacctttgg ttgagagcgc aaacacagag
     1141 gaaccgctcg gctgacgact ggtaaaggcg tcggcatgga tagagacaaa caggtcagca
     1201 cgctgtttct gggcttttgc tacgcgcact tgcaacggaa tgaagatgtc ttcattgcgc
     1261 gtcatgtaca ccttcatatt gccctctttc tcgatcagag agcgcagacg gcgagctatt
     1321 tgcaatacca cgtctttttc gcgcgttttg tatttcccca ccgcaccgga gtcttcgcca
     1381 ccgtggccag ggtcaagcat aatgacaata ggacgatcgc gccctgcttt acccggttgt
     1441 ggaccacttt gtgctggcgg cacctgcttt tcgaggtcgc ctttgttgta atcctccagc
     1501 agcgccagca gcgggtcctg catatcctgt gcattggccg gatagaggtc catcaccaga
     1561 cgctctttaa acccggcgac cggcgcaagg gcaaacagct gcggttttac gttttgcttt
     1621 aattcaaaaa ccatacgtac ggtttgcggg tcaaattgcc cgacgcgcgc cgacttgatg
     1681 aacgggtcgt cagcgcggat ttgcgcagcc atccccttga gtaccgagtt caggtttaca
     1741 tcttcgatat ccaccaccac gcgttcagga ttactcaacg cgaactgctt atatttcagc
     1801 tgacgatttg attctaccgt cacgcgggtg tagctggacg caggccagac gcgcaccgcc
     1861 acgacctggc tgaccgcagc caggctgacc tgacttacgc tcaatagcca catggcaccc
     1921 gcgccttgca gtaaacgacg acggctgatt gcagtgttgg atcctgacat gcctctcccg
     1981 agcaaaagaa atctaagctg tgtaacaagt aaacgaccaa tttgaccgga aactttagcg
     2041 aaagacgcat aatctgtcat ctataaaacg gtaaacattc tttttatatt cacggtatta
     2101 ctgataaaaa actcgctccc gcataaaatt tacacttgca ccctgcgaaa aaacagaata
     2161 aaaatacaat aatttcgaat aatcatgcaa agaggtgtac cgtggtaaag gaacgtaaaa
     2221 ccgagctggt cgagggattc cgccattcgg ttccctatat caatacccac cggggaaaaa
     2281 catttgtcat catgctcggc ggtgaagcca ttgagcatga gaatttctcc agtatcgtta
     2341 atgatatcgg gttgttgcac agcctcggca tccgtctggt ggtggtctat ggcgcacgtc
     2401 cgcagatcga cgcaaatctg gctgcgcatc accacgaacc gctgtatcac aagaatatac
     2461 gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc tgcgggaaca ttgcaactgg
     2521 atattactgc tcgcctgtcg atgagtctca ataacacgcc gctacagggc gcgcatatca
     2581 acgtcgtcag tggcaatttt attatcgccc agccgctggg cgtcgatgac ggcgtggatt
     2641 actgccatag cgggcgtatc cggcggattg atgaagacgc gatccatcgt caactggaca
     2701 gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt cactggcgag agctttaatc
     2761 tgacctcgga agagattgcc actcaactgg ccatcaaact gaaagctgaa aagatgattg
     2821 gtttttgctc ttcccagggc gtcactaatg acgacggtga tattgtctcc gaacttttcc
     2881 ctaacgaagc gcaagcacgg gtagaagccc aggaagagaa aggtgattac aactccggta
     2941 cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag cggcgtgcgt cgctgtcatt
     3001 taatcagtta tcaggaagat ggcgcgctat tgcaagagtt gttctcacgc gacggtatcg
     3061 gtacgcagat tgtgatggaa agcgccgagc agattcgtcg cgcaacaatc aacgatattg
     3121 gcggtattct ggagttgatt cgcccgctgg agcagcaagg tattctggta cgccgttctc
     3181 gcgagcagct agagatggaa atcgacaaat tcaccattat tcagcgcgat aacacgacta
     3241 ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat tggggaaatg gcctgtgtgg
     3301 cagttcaccc ggattaccgc agctcatcaa ggggcgaggt tctgctggaa cgcattgccg
     3361 ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct gaccacgcgc agtattcact
     3421 ggttccagga acgtggattt accccggtgg atattgattt actccccgag agcaaaaagc
     3481 agttgtacaa ctaccagcgt aaatccaaag tgttgatggc ggatttgggg taagtggtgg
     3541 aaatcgccag atgcgacatg cgcaatgcct ggaggcatga taaacacgct agcatcgcat
     3601 caggtattga cgctcatcgt cggatgcggc gtgaacgcct tatccgacct acatcaaatc
     3661 aaaggtgttg atggcggatt tagggtaagc gacggaaatc gccagatgcg catatgcgca
     3721 atgcccggag gcatgacagg acgcgcatgc gtcgcatcag gcaatcacgt ttattcccgc
     3781 gaactaaaca atgccgccag accactgcgc cgctcagtgc gcgtggcaat tgccgcactt
     3841 aaaatgcgct catcggcata cagcgacaga cggcgacgcg cgcgggtcac tgcggtgtaa
     3901 accagctctc gcgttactac cggcgtgcgt tgactcggta aaatcaacgc cgcatggtcg
     3961 aactccgatc cctgcgattt atgtaccgtc atcgcccacg tcgtttcgtg ctctggcagg
     4021 cgactcggtt gcacagactt aatattgccg tccggcatcg caaaccaaac gcgcgtcccc
     4081 tgcccgcgat ccagcgcaat accgatatcg ccattaaaca acccaagcgc gctgtcatta
     4141 cgggcaatca tcatcggtcg gccttcgtac caacgagagt gcggattacg atgaattttg
     4201 cgcttctgtt gcataaactg ctcaattcgc tcattcagtc cagccacgcc aaacggccct
     4261 tcccgcaggg cgcacaaaag ctggtactca ttgaacgcct gaatgattaa atccggctcg
     4321 gcacgcgctt gcagcagatc cagataacgc ccgtaacccg caagagcctc ctcaagcatc
     4381 gcaatatagt cttcgccgct ctgtaagagc cgtttttcga tatcagtaaa atcctgctga
     4441 aaaacggttt tcactgccgt tttatcacca cggttgatcg ccgcagctaa ctgaccaatg
     4501 ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc agagactgtc gcgcaaagat
     4561 gccgcttctg tgccagttcc tgacggaacg tgagttcccg tcaggcggct caactgcccg
     4621 gcacgctcgg cggtaaagcc cgcgttggca taagcgcaga tatcgcccag cacagctcca
     4681 gcctcaactg aggccagttg atcgcgatca ccgaggaaga tcactcgcgc atgatcgggc
     4741 aacgcatcga tcaggcgcga catcatgggg agatcgatca ttgacgcttc atccaccacc
     4801 agcacatcaa gatgcagcgg gttaccggca tgatgacgta aacgctggct acccggctgc
     4861 gcacccagca atcggtgcaa agtgctggca tcttccggaa tgcgtttctt ttgttcatcg
     4921 gtcagcggta actggcgcaa agccttgccg agagattcgg ttaagcgcgc ggcagcttta
     4981 cccgttggtg cagccagacg gatacggcag cgttcgccgt ctgccatttg aattaacgct
     5041 gccagcaact tcgctacggt ggtcgtttta ccggtgccag ggccgccgga aatcaccgag
     5101 atccgccgcg tcagcgccac tgccgccgcc actttttgcc agttaatttc atcgcttact
     5161 ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat caacctcaat ggcatgattc
     5221 acttcgttga aaaagcgtgc cactgtgcgc tcgttacacc acatgcgatt caaataaaga
     5281 cgatcaccac agaggatcat cggcgttggt tcatctcccc ggctgaccgc ttgtgaagcc
     5341 agcaagcatt cttcccaatt ttgtaactca ccgatttcgc tgacgcaggt tgctaacagc
     5401 ggatgtgacg cctcgttatt ttccagtcgt gagagcggta aacagacatg cccttccccg
     5461 gcgtcgtggc ttaacagggc tgccgcgagg gtgacggcag gatgttcatc tcccgccacg
     5521 gtcagggcaa attgcacatc cagcgggcgt aactgtttgt gctccacagc ttccagtaat
     5581 tgcttttgca atttcattac gcctcctcca gggtgatacc ggcaaacatc tcatccatca
     5641 ggtcaatcaa cccggcgttg ggtcgggtcg cgtagatccc ttgttgcgga tgttctttat
     5701 caacaccacg caggaacaga taaataacgc cgccaaagtg gcgctcatag tcgtaatcag
     5761 caatgcgatg gcgcagataa cggtgcagcg ccagggtata aagctgatat tgcagatcat
     5821 agcggtgtgc ctgcattgcc gctgccatag cctgttgggt gtaagcagaa ctgtcttcac
     5881 ccaaccagtt ggatttatag tcgagcaggt aataacgccc ttcgtggcgg aacaccaggt
     5941 caataaagcc ttttaacatg ccgcgtacct gcatgaactc cagcggcggg cagccagcgg
     6001 atagcgagtc aaactggcga attagtgcat caagctggct ggcgatgagc ggttcgctaa
     6061 tcggcaggta aaactccatc tccacctgtt tatcgcggtc ggaaagctga ctcaggctaa
     6121 cacccgtttc attgagaggt gcctggagga cagccgtgat ccactcggtc aacaccggtt
     6181 cccactgcga ttcaaagccg ccgagttcca gtttttcctg tacccagttc gggtcaaccg
     6241 gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa tgtccccggt gacgcaccgc
     6301 gcgggaactg atgcggtgtt aacgtcggtt cgtcaacgac gctgaccacg cctgcggcat
     6361 caacatccag ccgtggcatc agatcctgag cgataccgtg accacgctgc tgcaaaccgg
     6421 agtagctggt gacacgccag ttatcgccgg gcaatcgttg tagcgtcctc gcattcagtt
     6481 ctgcagttat agcatcatta acctgccagg gctggttatc accagtttgt gccgtttgcc
     6541 aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc gcatcttgcg
     6601 gttccccttt ttgcagcaaa cgcccgagcg cactttggtg gacgtcggtg tcaccttttt
     6661 tatcgccacg acggcgcacc agcggtgcaa cgccgagact gcaatgccaa accgaacgcg
     6721 tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg ttcggcctcc gcgagatcga
     6781 cgctttctgg cgcagcatta agatccagaa cagcctcaaa cgagtggcga tcgtgataga
     6841 acgcctgatc ctggacgcgg aaattggtaa taaatggcaa ccagaccagc ggatattcca
     6901 gcccttttga tttatggatc gtgacaatct gcaccagatg tttatcactt tcgaggcgca
     6961 tttgttggct ggaggcatta ctgtctggct cgaggatatg ttgcgataac cagcgtacca
     7021 gcgcatgttc actttccagc tgcgttccgg cttcttgtag caattcgctg atatgcaaga
     7081 tatcggtaag acgccgctca ccgcctgctg ttgccagcaa gttttctgca atgttacgcg
     7141 ccgacatcag cgcccgcagc atcggcataa cgccacgttt gcgccagatt tgccgataac
     7201 catcgaactc ttcgacgacc gcatcccacg catgttcgtc attattcagc gtttcaatat
     7261 ccagcgcatt cagccccatc attgacgttg ccagcgcact gcgcagggtg ttctcacgtt
     7321 cgggagtcat caccgcctgc aacagccaaa gcatctcctg cgcttccaga gtttcaaaaa
     7381 cactgtcgcg gttcgaaagg taaacggaag ggatttccag cagcattaag gcatcgcgca
     7441 cctgggcggc ctcctggcgg ctgcgcacca gcacactgat gtccgaagca cgcaccggac
     7501 gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg tccggcttgt agccagtcgc
     7561 ggatttgcgc agcacatacc tgcgccatgg tactttgata gtcgccaacg ccgcagcttt
     7621 cgccttccat cagccacatt ttcatcgcag gctgcgtttc acctttaaac acaaaacgta
     7681 acgcctgatt tttgccggca gatttcacag gaataaacgg tatttcacga aacatgaacg
     7741 cgtcatcagt ctggctgaaa agtttgttaa cgctgttcac cattcccggt gcggaacgcc
     7801 agttggtatc taaggtgtag tgagcatgaa cttcgctacg cgccttcata taagtgaaga
     7861 tatccgcacc ccggaatgca taaatagcct gtttcgggtc gccaatcagc aacaatgcgg
     7921 tttccggctg atggtgccag atacggcgaa aaattcgata ctgctggggg tcggtatcct
     7981 gaaattcatc gatcattgcc accgggaatc gcgtacggat cgccgctgcc aatacctcgc
     8041 cgctttcact acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa cccaattcgc
     8101 cacggcggcg tttttcacgc gctactgttt cgcggatctc agccaatgcg cgggtgatca
     8161 ccagatcgcg gatcgacaat ggttctgcaa gcagttgatc gatcgcctca aacagtggat
     8221 gtcgcggggt ttcccccccg gctttcgtgc gatcttctaa gaaacgctga gagaattttt
     8281 ccagcgactc cggcaactga tagctgttgg tctcttcttc tgcccaggcg ctgatcttgt
     8341 cgatccattt agcctgattg ctacggttaa acttgcgtcg atcaatacca gaagattcga
     8401 tcagcgcatc cagttcaccc actgcgtcgc gccactgctg ttttacggca tcaatacgcg
     8461 ccacaatttg cgcgtggcgg gaagcaagcg tttcatcatc gggcggcggt gctttgataa
     8521 ccggtgcttc gccttgcaga taacgattaa tatcgcgcag caacgcctgc ggccctttcc
     8581 aggtttcaaa gaccacctgg gcaatttcac gcggcagcgg gtagcagtgg cgacgccaga
     8641 aatcggcgca ggcctggtag cgtagcagag actcatcttc aatcagctgc tgctcaaaca
     8701 gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg gcaaaaaccg tgaatagtaa
     8761 agactgccgc ttcatccatc tgccgttcgg ctaacaacaa ccactgcgcg gcttgcgctt
     8821 tatcgtcgat ctcctccagc aggagtttgt acagtggatt gtcggtggtt tcacgcagac
     8881 aggcgatgcg caactcgtgg atattgctac ggatacgacc gcgcaattct gccgtggcag
     8941 cctcggtaaa ggtgaccacc agcagttctt caacggtcag cgggcgggga aaggcggcag
     9001 aaccgcctag tccaagtaac aggcgcaaat agagcgccgc aatcgtaaag gttttgcctg
     9061 tgccggcaga ggcttcaatc aggcgctcgc cctgtaaggg caagcgcaaa ggatctagtg
     9121 tctcggcgac atcactcatt cttttcactc atcaggggca ttgtttgctg caacgcgctg
     9181 acgttctccc acactttcca gccttcagga tgcacatatt cggctttccc gttctggctg
     9241 ccggaaatct gcgacagaat agccatgcct tgcggctcga ccaccgcctg atggaagaaa
     9301 tcagcaagtt tttgcggcgt cagcagtttt atctgggcca cgattttatc acgcgaatcg
     9361 aagcgcatat tgccgcgatc gaaatcttta cttaacttcg atgcttcttc gccgagcgtt
     9421 tgcggtgcct gcagcatctg ggttattacc gcctgctgga tttgcgcaaa ctcctctggc
     9481 ttcatcgccc gcaattttgc ctctgcggtt gggaaaaacg ccttgtaacg ctcccacaag
     9541 aatgaaggct gtttatcatt gctttgcaaa aggaagccca tgccccactg acgccccacg
     9601 ctcattggaa acgcaaacac ggcatagccc aactgttctt cggtacgcaa ctgattgtag
     9661 aaccacggct gtacgatctg ccccaacaga gagctatagg ctgagctggt gtattcatcg
     9721 tagccagtcg gtacaaatac cgctgccagt gcggagtcgg tgctgttacc ggctttttca
     9781 aagatgacag attgtttttt atcgaccacg acatctttgt tacggcacca ctccgaacca
     9841 tcagcgccca actgtttttg cacatcgcgt gccagcgttg ttgcctgggc ttcggtcatg
     9901 ttgccaataa ccataaactc tggtcgagcc cctgatttta aggcgtcgcg ataggccagc
     9961 acctctttca aagtgattga aggcaaaatt ttgcgccgtt catctcgcga gaagtacggc
    10021 acttgcgaga gcatctgcgc gggcataatc gcctgctcga acgctttgcc cttttctgcg
    10081 gaatccatca tctggttata ccaggacttc gcctgctcaa gctgatcttc cgtagcggta
    10141 tagctaaagt acccctcgag caatgcctgg aacagctgcg gcagacgctg ggtgtaacca
    10201 ttagcattaa ccataaggcc gttgttagcg ttggtggaaa aactaatgcc accaaccgac
    10261 gcctggttgc ttaactgatc aagcgctagc ccggcaagat aatcattgag cgcaaacatc
    10321 acctgattgc gggcgctgtc catggctttc ggattacgca aaatcaggct gacatcagct
    10381 ttgggttcgc tggaaaaata acggcttggc gcatagacca cacgcagatt cgactcatca
    10441 acaataagct ctggatggtc atatttcttc tctgacttaa tcagcgagaa atcgtcagga
    10501 atataggggt taagctctgg caaagagagc gcaatgtcgg cggctttttt ctgccagtca
    10561 gcgaaagttt gtgcgctaat tttatcgacc tgatacggcg catcgacaaa gtaagccgtt
    10621 ttgttgtgcg gctctttcgg gctgatatac cagatacgcg cattctgcgg cgtcatcatc
    10681 gccaggcgtt cttttactgc tttagcgtcg taccgatcgg caatattgac tgcatccagc
    10741 gtatgctcaa caggaacgcg aatcatggta tctgccagcc attcgacgta atccatatca
    10801 cgggtgattg acggataacg aaagtcgata tccagcacat tcgccagttc atcgaagtat
    10861 tgtttatcaa tccctttttc gcgtaacaga ttgaggtagc taaaaatggc cgccacaacc
    10921 tgatcgcgat tcgccagacc tttatcggtt aaagacgcag agatcgctaa tacgccgctg
    10981 ttgccgttga cgataggatc ggaattggcg ctaatgccct caactaatcc ctgcttttgc
    11041 agccagtcag aaagtgtacc tgggctgcga ttaccgatca gataggtaat caattcatcc
    11101 gttttactac ggaacttcgc tgaattgtta tcgatgcgaa actcaacgcg cagaacttta
    11161 cgcggcagcg ccgggacgta atgaatgata ataccttttt gcgcgtcggt gactaccggc
    11221 acggtgattt ccggtttttt gctctctttg ttcggcacgc gaccaaaggt gtccgccgcc
    11281 atttttgcca actccggcag cggtttattg ctgtaaataa ccgccttcat cagattggca
    11341 gagtaatatt tctcgtgaaa atctttcagc gcctgctgca ccggatttcc gggtttgtcg
    11401 cttaaagttt cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg gttaatggtt
    11461 tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac gcgccatggt taattcggca
    11521 ttcactgcat tacgttcacg ttcggcgtat ttcttgtcga gcaagggttc tgcaatagcg
    11581 tccgccaggc ggtctaccgc accgggcaag gcgtcgttct caacttccag atagaaagcc
    11641 gtgcgatacg gtgccgtgct ggcattgtga ctgccgccgt gcattttgag atattcggcc
    11701 agactgtcag cctgcgggta ctttttcgac cccatcagac tcatatgttc aaggtaatgt
    11761 gccagcccct ggtacgcctc gggatcttcc agcgacccaa cgggcactac cagcgccgag
    11821 agcgatttaa ccgcctgcgg atcagaaacc agcaagacca ccataccgtt atccagacgt
    11881 atagcctgat actggcggtt atctttatca cttttacgga tggtttcctg aatcggctgc
    11941 catcccgttt ctgcctgact taagggtgcc caaagggcaa caaacaacaa taatgctttg
    12001 aaccaggtgc tgcggggcat tcacggacct cataatcaac tttattttct ggccagattc
    12061 aacaggttaa cgcaaatcat ctgccagaat ttaatcttgt gctgaacgag tcagcctatg
    12121 tctatatatc catcagtccg tgactggtgc gcatcataaa gcaagcagat atattgcgca
    12181 atttttatac agcactcatg actgattaaa gcgaaacagc ggtaacagga aacgttgcga
    12241 ctgttcaacg atagcctcca ttgtctctgg tgttaattgc cgccagagcc gttgatacca
    12301 gatatcatca ccttcgccac gcaccatcat gttgccttcg taagcctgaa ggaatttcgt
    12361 acgggctttt tgtaacgtgg aatcgtcatc cagcatggca tcgttttgcg cgtcataaca
    12421 ggtttttagc cacgcgccgc cactttcagg taacaccagc aatggcgcgg acattccttc
    12481 acgatacccc tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg caagcggcgg
    12541 aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt tcaccattac caccgctggc
    12601 acagtagaca aggtgttcca gccaaagttg cattccctgc gccacactta ataaagaggg
    12661 acgccagcgc aacaggccat ccggctgcac ctgcggcaac cagccagtta tctgcacacc
    12721 gttgcaggcg agatcaattt ccatactctg ccccggctgg cgacgtgcaa tgactctgtc
    12781 ggcaagctgc tgcatctcct ggcactgtgt ttcccagaaa atttcaccaa aagcgccata
    12841 cggtaaatcc cctgccgccc ggaagcggcg gaataagcga tcggcatcat cctgctcaac
    12901 cagtgcattc aataactgct gattgatttg ataacggcta agcccttcca gaataaatgg
    12961 ctcggtgtcg gggatttcgc tgtcttcagt acggaagttc acctgcaaac gcatctggaa
    13021 gaacgcccgt accggatgtg cccagaatcg ttgtagcgtt tccagcggca cggtttccgg
    13081 taaggtaaac ggcagcggct gaacaaattc agaatgtgct ttaccagcct ggctggccgc
    13141 aggtagccat tcacgagcat agctttgtcg ttcgcctggc tggtagtttt gcggatcaaa
    13201 cggcatccgg gtatggaggc aagtaagatg cgcttttacc cttgcctcgc tttcatcaca
    13261 gttgagcgct tcatcgcccg gtagataatg gctttgcccg atgtagtcga tcagttcctg
    13321 caccagtacc gacgggaaac gttcactgtt atcctgaata gaacgcccga tatagctgat
    13381 atagagtttt tgctgcgcgg aaattaacgc ttccaggaac agatagcggt cgtcatcgcg
    13441 acggctacgg tcgccacgct tcggtttctg gctcatcagg tcaaagccca atggcgcaag
    13501 ctggcgtgga taaacgccgt cgttcattcc cagcaggcaa accactttga atgggatgga
    13561 acgcattggc atcagagtac aaatgttaac cggtccggcg agaaaacgct ggctgatacg
    13621 ttcctgatcc aggcgctgtg ccagttcatc acgcaatagt gacagcggca ccgcgtcgcc
    13681 atactgcgca cctaaacctt cggcgataat cgcctgccat tgttgttcga tcagcgtcat
    13741 agccgcttcg gtttccgcat ccggcaggaa gaaggcgttg agcatatcgc gacaaaccgg
    13801 caaccactct tccagcggac gctcctgcgc cagcccgcga cgccagatgt ttagctgcat
    13861 tagcagtgaa gccagatgcc ccaccagttc tgcaattaag ccgctcgatt catcataagg
    13921 tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg tagcccagca acatacgcgt
    13981 caggccaaat cgccaggtgt gttgtccggt ggcggggagt tccagctcgc gaacgttgtc
    14041 gtcatctatc ccccaacgaa tgccggattc gttgacccac tggcgtaaat aacgcagccc
    14101 ttcttcggtg atgtcaaacc gcgccgccag caccggcaca tccagcagcg ccagcacatc
    14161 ctctgacaca aagcgactgt caggcagtga taacaggctg ataaacgctt ccaataccgg
    14221 atgtgactgc cgcgcccgac ggtcggaaat ggcgtaaggc aggtaacgat ccgcaggtgc
    14281 gctaccaaac acagcctgaa taaacggact gtagctgtcg atgtcggcca ccatcacgat
    14341 gatgtcgcgc ggagtcagtg tcgggtcttc ctccagcatc gccagcaggc gatcgtgtaa
    14401 aacttcaact tcacgctgcg ggctatggca aacgtgaaag gtgatactgc tatccagtgg
    14461 atcaagcggg cgtttgttat cgctacggga aaactcttcg atgtttacac cagcaacggc
    14521 gcggttttcc agttccagaa tgtcagactg aatgttatgc agcaggttat ctggcgtaac
    14581 atcgacaaaa gcgtccagct cctggctgct ctccagatca gaaaggagat aaatgtagtc
    14641 gcgcccaagc ttgccccatg aggccagcag cgggttgccg acatcctgtt caccatcgct
    14701 gttaaagagc tgcccggcat tttcgctgtc gcgaaatagc ggtaattctc gatcttcaaa
    14761 actgtgtcgg cgctggcgag tcagtagttt cgccagataa gcaggatctt taatgtcgcc
    14821 ccagtaataa cggcaggggt tggtaaacag gagatggatt tcaatatgtt tacccagcgc
    14881 ctggagtgcc tggagataaa caggcggtaa cgcggaaata ccgcatataa agacgcgcga
    14941 aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc gctgatagag
    15001 attggcgcgg tgccagcgcg gttgcccgag ttcatgggta tattccacca acgccttcca
    15061 caacggcgct tgccaggcct gtgcttctcc cagcccttca accaaatgtc ctatttccca
    15121 ctgtgccagc cagtccggac gatagaccag atactggtca aacaggtccg ccgcttttga
    15181 agaaagctgg aacagttttc gcttgtcgct atcgtcagtc agataatgcc gcaacagggt
    15241 aaagtcttcg cgctccaaca gttgcggcag cagagtcatc agtttccagc tcatgctctg
    15301 tttgttaaag gcgctctctt tggggatttc cggtaacacc cggacgaaca tatcccagat
    15361 aaagctcgct ggcagcggaa aatcaatgtt tgccgcaata ccaaactttt gcgacagggt
    15421 catttgcagc cactgcgcca taccggtact ttgcaccaga atcatttctg gttcgaaagg
    15481 atcgtccagc cgttcgcgtt cgacgataaa ctccatcaac gcttccagca cgtccagacg
    15541 attggaatgg tagaccctta acatagcggc tcctgactac tgacgattcg ggcaatgcag
    15601 gcgggtcatc tcgccttctc tgcccccggg tgaaactagc gtaacgctga tgctgacaca
    15661 tcccgcctgc gatgtctgca ttcggttgac ctgccagtta gcaggtggcg aaatcgcgcg
    15721 cagttgcgtt tgctgccagc catgccgcca gagctgttgg tactggtttc tgctggcaag
    15781 actgttcatt aatgttcgct ggaaacccga taacgcagtg acaatcatca ccatcaacac
    15841 catcgccaac attacctccg gcagactaaa gccttgttga ttcctcaggg aagctgacat
    15901 aacgccctct ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa aacaatgttt
    15961 ccatcgatga cttcgcctgt tcgccacaac gaaacgcctt cataaccagc aatcaataag
    16021 gcttcattat ctgccagtaa acgcagacaa acccgggcac cggtggcagc atacagtaag
    16081 cactgaactg cgggcagcgt ctgccaggag tgcatttttc cccactccag cgccgactga
    16141 acgatggcct ggcggcgcaa tgactgactt tccatgctca cgcgagaagc aaaactgcga
    16201 tcctgctgac tcattccttg caatagcaag ctacccaaaa tcagcagcat caggaccaga
    16261 gccagtgacg aaacaccctt ttcgcggttc acaggttgga tcctgtcacg ctatagctgg
    16321 catccaccac ggtttgcgga tcagccttgc tggcagcgtg catattaacc gtcaacaccg
    16381 gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt gtcgataatg atggcatccg
    16441 gattagtgac tttatcccag cccttacctt cacaggatgt cgcaccgcgt agcgtttcca
    16501 gcacatgctc cttcagacga aatccaatct ggtcggactc ttttaccggt tcgcgatccc
    16561 agataccatt actgttcgca tcccactgca caatgacaca gtcaccctgt ccgacaattt
    16621 ccagcccttc gcccgtacag ctgccatggc aataacccgc cctctggaga tgcttcgcga
    16681 cggtaaatac ccgcagccag atttcatctt ccagcgccag cttacgggtg ttcgttaaac
    16741 tttcacgctg taacgcaggc agaaagcgtg cagcccccag caacaatacg ctactgatcg
    16801 ccatagcaat caacacttcc agcagagaaa aaccttgctc ttttacaggc atccttctgt
    16861 ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa accactagcc accactcgcc
    16921 cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc caaagaaagc
    16981 aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag cgcggcacaa agaccaatgg
    17041 tgaactgcca tgacaggtat tcgccccagc aacggaactc acaaggcacc ataacgtccc
    17101 ctccctgata agactgatac tgtggtcgcg gttatgccag ttggcatctt cacgtaaata
    17161 gagcaaatag tcccgcgcct ggctggcggt ttgccaaagc cgttgcgact gctgccagta
    17221 ttgccagcca taaagcccac ttgcgcttag catgaccaga atcagcatcg cgaccagcgt
    17281 ttcaatcagc gtataaccac gttgtgtttt catgccggca gtatggagcg aggagaaaaa
    17341 aagacgaggg ccagtttcta tttcttcggc acatcttccg gactatttac gccgttgcag
    17401 gacgttgcaa aatttcggga aggtgtctcg aagaatttaa cggagggcaa aaaaaaccga
    17461 cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta gatagccacc ggcgctttaa
    17521 tgcccggatg tggatcgtat ccttcaatct caaagtcttc gaaacggtag tcgaagatgg
    17581 attcgggttt acgtttgata atcaacttcg gcagcggacg cggttcgcgg cttaattgca
    17641 gatgagtttg atccatatgg ttgctgtaga gatgcgtgtc gccaccggtc cagacaaaat
    17701 cacccacttc cagatcgcac tgctgcgcca tcatatgcac cagtaacgcg tagctggcaa
    17761 tgttgaacgg caggccgagg aagacgtcac aggagcgctg ataaagctgg caagagagtt
    17821 tgccgtctgc cacatagaac tggaagaatg catggcacgg tgccagcgcc attttatcca
    17881 gttcgcctac gttccacgct gaaacaataa tgcggcggga atccgggtcg tttttcagct
    17941 ggttcagtac agtagtgatc tggtcaatat gacgaccatc cggcgttggc caggcgcgcc
    18001 actgtttacc atacactggc ccgaggtcgc cgttttcatc ggcccattcg tcccagatgg
    18061 tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc ctgcaggaac cacagcagtt
    18121 cgtggatgat ggaacgcagg tggcaacgtt tagttgtcac cagcgggaat ccatcctgca
    18181 ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc ggttccggta cggtcgtttt
    18241 tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa atactgtttc atggttcctc
    18301 aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac catcatgatc acacccgcga
    18361 caatcatcgg gatggaaaga atttgcccca tgctgatgta ctgcacccag gcaccagtaa
    18421 actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg taaccaatca
    18481 ggaacaagcc tgagacagct cccattgggc gcggtttacg aatatacagg ttgaggataa
    18541 taaacagcac cacaccttcc agcagcagct cgtaaagctg tgatgggtgg cgcggcagca
    18601 caccgtaagt gtcgaaaatg gattgccact gcggattggt ttgcagcagc aaaatatctt
    18661 ctgtacggga gccagggaac agcatggcaa acgggaagtt cgggtcaacg cggccccaca
    18721 attcaccgtt aataaagttg cccaggcgcc cggcaccaag accaaacgga atgagtgggg
    18781 caataaaatc agagacctgg aagaaggaac gtttagtacg gcgggcgaag ataatcatca
    18841 ccacgataac gccaatcagg cctccgtgga aggacatgcc gccgtcccat acacggaaaa
    18901 gatacagcgg atcggccata aactgcggga aattgtagaa cagaacataa ccaatacgtc
    18961 caccgaggaa gacgccgagg aagcccgcat agagtaagtt ttcaacttca tttttggtcc
    19021 agccactgcc cggacgattc gcccgtcgtg ttgccagcca cattgcaaaa atgaaaccca
    19081 ccagatacat caggccgtac cagtgaagcg ccacgggtcc tattgagaaa atgaccggat
    19141 caaactccgg aaaatgcaga tagctactgg tcatctgtca ccacaagttc ttgttatttc
    19201 gctgaaagag aacagcgatt gaaatgcacg ccgcaggttt caggcgctcc aaaggtgcga
    19261 ataatagcac aaggggacct ggctggttgc cggataccgt taaaaaatat gtatatgatc
    19321 cgcgctataa ccccccgcga atcaacccgc ccatgccgcg acgctccata aaggctgcaa
    19381 cctgatggcg aacttcggtc gccagttgcg cttccagact acgctgcgca agattttctg
    19441 cttcagcaaa atcaatgcgc cgcagcaggt attttacgcg cgctacagaa cgtccgttca
    19501 tagacagatg gcgataccca agcccaatga ggattgccac gcacatggga tcgcccgcca
    19561 tttcaccgca caaacggaga tcgattccat gtatttccgc ttcccgggcg atcatcgcca
    19621 gagctcgtaa cattgcagga tgaagactgt cataaatgtt cgccacccgg gtattgttgc
    19681 gatcaacggc caggatgtat tgagtcagat cgttggtgcc aacagagatg aaatcgaccc
    19741 gctttgccag atgcggcagc ataaatacca ttgacggcac ttccagcatg atgccgatac
    19801 gtggtttggg aatttcgtaa ccgatcatct cctcgacttc acgtccggca cgttcaatca
    19861 ggcggcgtgc ttcgtcaact tcatcgaggc ttgtgaccat cggcaacaga atattcaggt
    19921 tgcccgtagc ggcattagca cgcagcatcg cccgcacctg gatcaagaag atctccggct
    19981 gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg attctcttcg ctaatcggca
    20041 tgtaaggcag ctgcttatct gctccgacat ccagcgtacg caaggtgacg ggtttatcat
    20101 taaacatttg cagcatcccc tgatactgcg ccacctgttc ttcttccgac ggaaaaccac
    20161 tttgcagcat gaatgggatt tcagtgcgat aaagtcctat gccatcaata cggctgccca
    20221 gtttttcttc atgttccggg cttaaaccgg cattgagcat gactttaatg cgctcgccgc
    20281 tttttaactg ggcgggtaaa ttgacgtcat cttccgccag acggctaagc tcgatctctt
    20341 cactaattag ccgctgatat tcttgcagca gtaccggttc cggatcgacc agcaattcac
    20401 cgcgataacc atcgacgatc agcgtccgac gatgcagtac cgaaggctga atatccgcgc
    20461 ccatcacggt aggtatcccc agcgcacgta ccatgatcgc agcatgggag ttggcagcgc
    20521 catcgcgcac gacaacaccg actaagcgat cctggggcag ctcagcaagc gttgtcgctg
    20581 acagttcatc tgccaccaga atgaaacgtt ccggccaggc gttcggccct tgattagcgt
    20641 catcaagatg aaacagcaat cgctgaccca gcgcacgtaa atcgccagcc cgctctttga
    20701 gatagttatc gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc gtttttaccg
    20761 cccactctgc caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc agccgggtat
    20821 ccgaaagcag gtgtgagtaa agatcgaaaa tagccgccgt ttctttttgt gcaccggcag
    20881 caaagcgttt gctatagcgg cgaaactcgt tagccgcctc ttccaacgcc ccggtcagtc
    20941 gttcgcgttc cagagccgga tccagcgttg atgcctgata cacctgttcc attaaaggta
    21001 acgtggcatc ctgccagcct tcggcaatcg ccacaccagg tgctgccggt aatgcacgga
    21061 ttcgcgtctg gcgatattgc ccaaacaagg cagtcaactg cgactgagaa agaatagctg
    21121 ccatctgggt ggcaagcgtc accaggaagg attcttcact ttcgtcatac tggcgcaact
    21181 ctcgttgctg taccaccagt acaccaagca actggcgacg ttgaataatt ggtacgccta
    21241 aaaacgcgcg gaaacgttct tcttttacgg aggggatgta tttgaagctg gggtgctttt
    21301 gcgcatctgc aaggtttatc ggttccgcca gcctgccaac caggccgacg atcccttcat
    21361 caaacgcgag cgttacagtg cgaccgcgtg gttttttcag tccccgggtc gccatcaggt
    21421 agtaacaacg tcgatcatga tcggccaggt agaccgaaca gacctcggta tccatcgcaa
    21481 gacagatgtc ggtaaccaga atatttaacg cctcattcag gcgtggtgcg ctggctacct
    21541 tttcgactat ttcgcgcagg cgagtgagca taattggcgt gacttaacct cttttacgtc
    21601 gataagcaga tgcgttttgt ggttttggcg tattttcctg cagtgacatc accacactcg
    21661 cgaactcttt cattacccta cggtagacat cacgtttaaa tgacaccacc tgtctgactg
    21721 gataccagta acttacccat cgccagccgt caaactctgg tgtactgctg gtttgcatat
    21781 tgatttctgc atcgccgctc accagctgca agagaaacca tttttgtttt tggccgatac
    21841 aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc aaccagttac
    21901 gcgttgaagc aaggattcga acgtctttgc ggcttaatcc tacttcttca aacaattcac
    21961 ggtacatcgc ctgctctgcg gattctccgg ggttgattcc gccttgcgga aattgccagg
    22021 agtgctgacc aaatcgccgg gcccacatta cctgcccctg gcgattacaa atcacgatac
    22081 ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact acctcaaaat aaagctttat
    22141 atacgaatga ttgtttcata ctccaggaag acggtaaacc actctctgca gggcatt
//
LOCUS       DAZUCC010000064        22128 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZUCC010000064 DAZUCC010000000
VERSION     DAZUCC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.064"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..2396
                     /gene="polB"
                     /locus_tag="ACHY09_002144"
     CDS             45..2396
                     /gene="polB"
                     /locus_tag="ACHY09_002144"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFS3885114.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGSIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            2560..5466
                     /gene="rapA"
                     /locus_tag="ACHY09_002145"
     CDS             2560..5466
                     /gene="rapA"
                     /locus_tag="ACHY09_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFS3885115.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            5478..6137
                     /gene="rluA"
                     /locus_tag="ACHY09_002146"
     CDS             5478..6137
                     /gene="rluA"
                     /locus_tag="ACHY09_002146"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFS3885116.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6255..7070)
                     /gene="djlA"
                     /locus_tag="ACHY09_002147"
     CDS             complement(6255..7070)
                     /gene="djlA"
                     /locus_tag="ACHY09_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFS3885117.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKLQKGFK"
     gene            7325..9679
                     /gene="lptD"
                     /locus_tag="ACHY09_002148"
     CDS             7325..9679
                     /gene="lptD"
                     /locus_tag="ACHY09_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFS3885118.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            9732..11018
                     /gene="surA"
                     /locus_tag="ACHY09_002149"
     CDS             9732..11018
                     /gene="surA"
                     /locus_tag="ACHY09_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFS3885119.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11018..12007
                     /gene="pdxA"
                     /locus_tag="ACHY09_002150"
     CDS             11018..12007
                     /gene="pdxA"
                     /locus_tag="ACHY09_002150"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFS3885120.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            12004..12825
                     /gene="rsmA"
                     /locus_tag="ACHY09_002151"
     CDS             12004..12825
                     /gene="rsmA"
                     /locus_tag="ACHY09_002151"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS3885121.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            12828..13205
                     /gene="apaG"
                     /locus_tag="ACHY09_002152"
     CDS             12828..13205
                     /gene="apaG"
                     /locus_tag="ACHY09_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFS3885122.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13212..14054
                     /gene="apaH"
                     /locus_tag="ACHY09_002153"
     CDS             13212..14054
                     /gene="apaH"
                     /locus_tag="ACHY09_002153"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFS3885123.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKHYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14132..14611)
                     /gene="folA"
                     /locus_tag="ACHY09_002154"
     CDS             complement(14132..14611)
                     /gene="folA"
                     /locus_tag="ACHY09_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFS3885124.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(14803..16665)
                     /gene="kefC"
                     /locus_tag="ACHY09_002155"
     CDS             complement(14803..16665)
                     /gene="kefC"
                     /locus_tag="ACHY09_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFS3885125.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGAVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(16658..17188)
                     /gene="kefF"
                     /locus_tag="ACHY09_002156"
     CDS             complement(16658..17188)
                     /gene="kefF"
                     /locus_tag="ACHY09_002156"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFS3885126.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(17296..18627)
                     /locus_tag="ACHY09_002157"
     CDS             complement(17296..18627)
                     /locus_tag="ACHY09_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3885127.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(18686..18973)
                     /gene="fixX"
                     /locus_tag="ACHY09_002158"
     CDS             complement(18686..18973)
                     /gene="fixX"
                     /locus_tag="ACHY09_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFS3885128.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(18970..20256)
                     /locus_tag="ACHY09_002159"
     CDS             complement(18970..20256)
                     /locus_tag="ACHY09_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS3885129.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(20307..21248)
                     /locus_tag="ACHY09_002160"
     CDS             complement(20307..21248)
                     /locus_tag="ACHY09_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFS3885130.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(21263..22033)
                     /locus_tag="ACHY09_002161"
     CDS             complement(21263..22033)
                     /locus_tag="ACHY09_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFS3885131.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         5316 a         6141 c         5783 g         4888 t
ORIGIN      
        1 aaaccaggct ataatcaagc ctggtttttt attggatttt cagcgtggcg caggcaggtt
       61 ttatcttaac ccgacactgg cgggataccc cgcaaggaac cgaagtttcc ttttggttag
      121 cgacggacaa cgggccgttg caggttacgc ttgcgccgca agagtccgtg gcgtttattc
      181 ccgccgatca ggttccccgc gcccagcata ttttgcaggg tgagcaaggc tttcgcctga
      241 cgccgctggc gttaaaggat tttcatcgcc agccggtgta tggcctttac tgtcgcgccc
      301 atcgccagtt gatgaattac gaaaagcgcc tgcgtgaagg tggcgttacc gtctacgagg
      361 ccgatgtgcg cccgccagaa cgctatctga tggagcggtt tatcacctca ccggtatggg
      421 tcgagggtga tatacgcaat ggcgctatcg ttaatgcccg tctgaaaccg catcccgact
      481 accgtccgcc gctcaagtgg gtttctattg atattgaaac cacccgccac ggtgagctgt
      541 actgcatcgg cctggaaggt tgcgggcagc gcatcgttta tatgctgggg ccagagaatg
      601 gcgacgcctc cgcgctcgat tttgaactgg agtacgtcgc cagccgcccg cagctactgg
      661 aaaaactcaa cgcctggttt gcgactcacg atcccgatgt gatcatcggc tggaacgtgg
      721 tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg ttaccgtatt ccgctgcggc
      781 tggggcgcga taacagcgag ctggagtggc gcgagcacgg ctttaaaaac ggtgtgtttt
      841 ttgctcaggt taaaggtcgg ctaattatcg acggtatcga ggcactgaaa tccgcgttct
      901 ggaatttctc ttcattctcg ctggaaaccg tcgctcagga gttattaggc gaaggaaaat
      961 ctatcgataa cccgtgggat cgaatggacg aaattgaccg ccgtttcgcc gaagataagc
     1021 ctgcgctggc aacttataac ctgaaagatt gcgagctggt gacgcagatc ttccacaaaa
     1081 ctgaaatcat gccgtttttg ctcgaacggg cgacggtgaa cggcctgccg gtagatcgac
     1141 acggcggttc ggtggcagcg tttggtcatc tctattttcc ccgtatgcat cgcgctggtt
     1201 atgtcgcgcc taatctcggc gaagtgccgc cgcacgccag ccctggcggc tacgtgatgg
     1261 attcgcgacc tgggctttat gattcggtgc tggtgctgga ctacaaaagc ctgtacccgt
     1321 cgatcatccg cacctttctg attgatcccg tcgggctggt ggaaggcatg gcgcagcctg
     1381 atccagagca cagcaccgaa ggctttctcg atgcctggtt ctcgcgagaa aaacattgcc
     1441 tgccggagat tgtgactaac atctggcacg ggcgcgatga agccaaacgt cagggtaata
     1501 agccgctttc gcaggcgctg aagatcatta tgaatgcctt ttatggcgtg cttggcacca
     1561 ccgcctgccg cttcttcgat ccgcgcctgg cgtcgtcgat cactatgcgt ggtcatcaga
     1621 tcatgcggca aaccaaagcg ttgattgaag cgcagggcta cgacgtgatc tacggcgata
     1681 ccgactcaac gtttgtctgg ttgaaaggcg cacattcgga agaagaagcg gcgaaaattg
     1741 gtcgtgcact ggtgcagcac gttaacgcct ggtgggcaga aacgctgcaa aaacaacggc
     1801 taaccagcgc actggaactg gagtatgaaa cccatttctg ccgttttctg atgccaacca
     1861 ttcgcggagc cgataccggc agcaaaaagc gctacgccgg actgattcag gagggcgaca
     1921 aacagcggat ggtatttaaa gggctggaaa ccgtgcgcac cgactggacg ccgctggccc
     1981 agcagtttca gcaggagctg tacctgcgta tcttccgcaa cgagccatat caggaatacg
     2041 tgcgcgaaac catcgacaag ctgatggcgg gcgaactgga tgcgcggctg gtttaccgta
     2101 aacgccttcg ccgtccgctg agcgaatatc agcgtaatgt tccgcctcat gtacgcgccg
     2161 ctcgccttgc cgatgaagaa aaccaaaagc gcggtcgccc cttgcaatat cagaaccgcg
     2221 gctccattaa gtacgtatgg accaccaacg gcccggagcc gctggactac caacgttcac
     2281 cgctggatta cgaacactat ctgacccgcc agctacaacc cgtggcggag ggaatactcc
     2341 cttttattga ggataatttt gctacactta tgaccgggca acttgggcta ttttgagcaa
     2401 aaaaagagtt cgccagatac cattttgatg cgtgacgaat gctttgccat ccagtaccat
     2461 agcgcccttt ccattcctgg acctgaataa caccactacc tcataagcac ggtagcgggt
     2521 ggttattgcc tgcaattaaa gatatagagc cgaacacata tgccttttac acttggtcaa
     2581 cgctggatca gcgatacaga aagcgaattg ggacttggaa ccgttgtcgc ggtggatgcg
     2641 cgaactgtca ctttactttt cccatctact ggtgaaaacc gtctgtacgc acgcagtgat
     2701 tcccccgtga cccgcgtgat gttcaaccct ggtgatacta ttaccagcca tgacggctgg
     2761 cagatgcaag tcgaagaagt aaaagaagaa aatggcttgc tgacctatat cggtactcgc
     2821 ctggatactg aagagtccgg cgtagccctg cgtgaagttt tccttgatag caaactggtg
     2881 ttcagcaaac cgcaggaccg tctgtttgcc gggcagattg accgtatgga ccgctttgcg
     2941 ctgcgttatc gcgcgcgtaa gtattccagc gaacagttcc gtatgccgta cagcggcctg
     3001 cgcggtcagc gtaccagcct gatcccgcac cagctcaaca tcgctcatga tgttggccgc
     3061 cgccacgcgc cgcgcgtcct gctggctgac gaagtgggtt tagggaaaac cattgaagcc
     3121 gggatgatcc tgcatcagca actgctctct ggcgctgctg aacgtgtgct gattatcgtc
     3181 ccggaaacct tacagcatca gtggctggta gaaatgctgc gccgtttcaa cctgcgcttt
     3241 gctctgtttg atgatgagcg ttatgccgaa gctcagcacg atgcttacaa cccgttcgat
     3301 accgaacagc tggtgatttg ctcgctggat tttgctcgtc gtagcaaaca gcgtctggaa
     3361 catctctgtg aagccgaatg ggatctgctg gtggtcgatg aagcccatca cctggtgtgg
     3421 agcgaagatg cgccgagccg cgaatatcag gccattgaac aactggcaga gcacgtgccg
     3481 ggcgttctgc tgctgaccgc aaccccggaa cagctgggga tggaaagcca cttcgcccgt
     3541 ctgcgtctgc tggacccgaa ccgtttccac gattttgcgc aattcgttga agagcagaaa
     3601 aattatcgtc cggttgcgga cgccgttgcc atgctgctgg caggtaacaa actgagcaat
     3661 gatgaactga acatgctcgg tgagatgatc ggcgagcagg atatcgagcc gctgttgcaa
     3721 gcagcaaaca gcgacagcga agatgcccag agcgcccgtc aggaactggt ttcgatgctg
     3781 atggatcgcc acggcaccag ccgcgtgctg ttccgtaaca cgcgtaacgg tgtgaaaggc
     3841 ttcccgaaac gcgagctgca tactattaag ctgccactgc cgacgcagta tcagaccgct
     3901 attaaagtct ccggcattat gggcgcacgt aaaagtgcgg aagatcgcgc tcgcgatatg
     3961 ctctacccgg agcgtattta tcaggaattt gaaggcgata acgccacctg gtggaacttc
     4021 gatccgcgcg ttgaatggct gatgggctac ctgaccagcc atcgctctca gaaagtgctg
     4081 gtgatctgcg ccaaagctgc cactgcgctg caactggagc aagtactgcg cgaacgtgaa
     4141 ggtatccgcg ctgctgtgtt ccacgaaggc atgtcgatta tcgaacgtga ccgcgctgcc
     4201 gcctggtttg ctgaagaaga caccggtgcg caggtactgc tgtgctcgga aatcggttct
     4261 gaaggacgta acttccagtt cgccagccac atggtgatgt ttgacctgcc attcaacccg
     4321 gatctgctgg agcagcgtat tggtcgtctg gatcgtatcg gtcaggcgca cgatattcag
     4381 atccatgtgc cttatctgga aaaaactgct cagtcggtgc tggtgcgctg gtatcacgaa
     4441 ggtctggatg cattcgagca cacctgcccg accggacgca ctatttacga tagtgtatac
     4501 aacgatctga ttaactatct ggcttcaccg gatcaaaccg aaggctttga cgatctgatc
     4561 aaaaactgcc gcgagcaaca tgaagcgctg aaagcacagc tggaacaagg tcgtgaccgc
     4621 ctgctggaaa tccattccaa cggtggcgaa aaagcccagg cactggcaga aagcattgaa
     4681 gagcaggatg acgataccaa cctgatcgcc ttcgccatga acctgttcga tattatcggt
     4741 atcaatcagg acgatcgcgg cgacaacatg atcgtgctga cgccgtccga tcatatgctg
     4801 gtgccggact tccctggcct gtcggaagat ggcatcacca tcaccttcga ccgtgaagtg
     4861 gcgctggcgc gtgaagatgc acagtttatt acctgggagc atccgctgat ccgcaacggt
     4921 ctggatctga tcctttctgg cgataccggt agcagcacga tttcactgtt gaaaaacaaa
     4981 gcgttgccgg taggtacgct gttggtggaa ctgatttatg tggttgaagc ccaggctccg
     5041 aagcagttgc agctcaaccg cttcctgcca ccgacgccgg tacgtatgct gctggataaa
     5101 aacggcaaca acctggcggc gcaggtagag tttgaaacct ttaaccgcca gcttaacgcg
     5161 gttaaccgtc acaccggcag caagctggtt aacgccgtgc agcaggatgt tcacgctatc
     5221 cttcagctgg gggaagcgca gatcgagaaa tctgcccgtg cattgattga tgccgcacgt
     5281 aacgaagccg acgaaaaact gtctgccgag ctgtctcgtc tggaagcgct gcgtgcagtt
     5341 aacccgaaca ttcgtgacga cgaactgacc gccattgaga gcaaccgtca gcaggtaatg
     5401 gaaagcctgg atcaggcagg ctggcgtctg gatgccctgc gtttgatcgt tgtgacgcat
     5461 cagtaacgga gccgaagatg gggatggaaa actacaatcc gccgcaggaa ccctggctgg
     5521 ttgtcctgta tcaggatgac catattatgg tggtcaacaa gccgagcggt ttgttgtctg
     5581 tgccgggtcg tctggaagag cacaaagaca gcgtgatgac gcgcattcag cgtgattatc
     5641 cgcaggcaga atcggtgcat cgtctggata tggctaccag cggcgtgatt gtagtggcgc
     5701 tgaccaaagc cgcggagcgg gagttaaaac gccagttccg cgagcgcgag cctaaaaaac
     5761 agtatgtggc ccgcgtctgg gggcatccat cccctgcgga aggtctggtg gatctgccgc
     5821 tgatttgcga ctggccaaac cgcccgaaac agaaagtctg ttacgaaaca ggtaaacctg
     5881 cgcagacgga gtatgaagtg gtggaatatg cggcggataa cacggcaaga gtggtgttaa
     5941 aaccgattac cgggcgttcg catcagctgc gtgtgcatat gctggcgctg ggtcatccga
     6001 ttctcggcga tcgtttttat gcatcaccag aagcgagagc aatggcacca cgtttgttgc
     6061 tacatgcaga gatgctgacg attacccatc cggcgtatgg caatagtatg acgtttaaag
     6121 caccagcgga tttttaatag tcacgctggc gaagttcggt aggccggaga aggcgttcac
     6181 gccgcatccg gcaattttgc gccgattgcc tgatgcgacg cgggccgcgt cttatcaggc
     6241 ctacacgttg aaacttattt aaaccctttc tgcagcttaa tcaattcata ggcctgctga
     6301 atttcctgcg ctttttgctt cgccatctcc atcatctcag gcggcaaacc tttcgccacc
     6361 agcttatcgg gatggtgttc actcatcagc ttacggtagg cacgtttgat ggtggtcgca
     6421 tcatccgtcg gcttcacgcc cagcacatta caggcatctt ccagcgttgg gccacgctgc
     6481 gcttgctgcc agttaccacc gccagattgc tgctgataac cgccgccaaa ctgtgcaccg
     6541 ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa ttcttcagca
     6601 atgacataca gcacagcccg ttcattcggg tgcagtgaac catcagcaaa cgccgcctga
     6661 atctggatct ccagaaacat acgaattaag tcaaaacgac caaagcagac actgcgaaac
     6721 tggcgcatct tttcgcgcag cgggtaattg tctgattttc ccacccggaa cgcgttttgc
     6781 gccgcagtac gggaagcgcc atgaaggttc attcggtcca tcaactggct ggcgatatga
     6841 atatcagcct ccgtgacgcg acctttggat ttggttaaat gccccatcac ttcaaaagtg
     6901 gtggcaaaaa acagcgcctg acgctcacgc tggttggcga accacgccat tttacggcta
     6961 cgggctttat caaacatatg gccaattaac aggcctaaca ctacgcccca aaagccgccg
     7021 cccatcagta aggccacggc cacgccaatg atttttcccc aatactgcat atattcccca
     7081 aatcgacaca cggatatcag ggctatctcc cacaatataa aggtgctttt accgttttcc
     7141 ggctgcggtc aattgtggga catcgcctat aatttgcatt atcatacctg tcattcaatg
     7201 ccgtgcctaa caccactgac gctattcgga caggattaac actagcgtag agatgacgag
     7261 tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac aataaaatca
     7321 acgtatgaaa aaacgtatcc ccactctcct ggccaccatg attgccaccg ccctttatag
     7381 tcaacaggga ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc caagctatga
     7441 ccgtcctctg gtacagggcg ataccaatga cttacccgtg actatcaatg ctgaccacgc
     7501 gaaaggggac tacccggatg acgccgtgtt tactggcagc gtggatatca tgcagggtaa
     7561 cagccgtctg caggccgacg aagtgcagct ccatcaaaaa gaggcgccag gacaaccaga
     7621 gccggtacgt accgttgatg cgctcggtaa tgtccattac gacgataacc aggtgatcct
     7681 caaagggccg aaaggctggg cgaatctgaa caccaaagat accaacgtct gggagggtga
     7741 ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac ctgatgaaac aacgtggcga
     7801 aaaccgctat accattctgg ataacggtag ctttacctcc tgtctgccgg gttctgacac
     7861 ctggagcgtg gtaggcagcg aaattattca tgaccgcgaa gaacaagttg cggagatctg
     7921 gaacgcccgc tttaaggtgg gtccggtacc gatcttttat agcccctatt tgcagttgcc
     7981 ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac gccaagtaca ccaccaccaa
     8041 ctactttgag ttctacctgc catattactg gaacatcgcg ccaaatatgg atgccaccat
     8101 cacgccgcat tatatgcatc gtcgtggcaa tatcatgtgg gagaacgaat tccgctacct
     8161 ctcccaggcg ggcgctggct tgatggaact ggactatctg ccttcagata aagtctatga
     8221 agatgaacac ccgaacgatg acagttcacg tcgttggtta ttctactgga accactccgg
     8281 ggtcatggat caggtgtggc gtttcaacgt cgactacacc aaggtcagcg atcctagcta
     8341 cttcaatgat tttgataaca agtacggttc cagtactgac ggctacgcaa cgcaaaaatt
     8401 cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc agttccaggt
     8461 cttcagcgag cagaacacca gtagctactc ggcagagccg cagttagacg ttaactacta
     8521 ccagaatgat gttggtccgt ttgatacacg catttacggc caggcagtgc actttgttaa
     8581 caccagagac gacatgcctg aagcaacccg tgttcacctg gaaccgacca tcaatttgcc
     8641 gctctctaat aactggggca gcatcaatac cgaagcgaag ttgctggcaa cccattatca
     8701 gcaaactaat cttgactggt ataactccag aaacacaacc aagctggacg aatccgttaa
     8761 ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc tttgaacgcg atatggaaat
     8821 gctggcgccg ggttataccc aaacgctgga accgcgcgcg cagtatttat acgtgccgta
     8881 tcgcgatcag agcgacatct ataactacga ctcgtctctg ctgcaatctg actactctgg
     8941 cctgttccgg gaccggactt acggcggtct tgaccgtatt gcctccgcta accaggtgac
     9001 gaccggtgtc acatctcgca tatatgatga tgctgccgtt gaacgtttta atatttccgt
     9061 tggtcaaatc tactatttca cggagtctcg cactggcgat gacaacataa catgggagaa
     9121 tgacgacaaa acgggctcac tggtgtgggc aggcgatact tactggcgta tctcagagcg
     9181 ttggggattg cgtggcggaa ttcagtacga tacacgtctg gataacgtag cgaccagtaa
     9241 ctccagcatt gaataccgtc gggatgaaga ccgtctggta cagctgaatt accgttacgc
     9301 cagcccggaa tatattcagg ctacgctgcc taagtactat tccactgctg agcaatataa
     9361 gaatggtatt tcgcaggtag gtgccgtcgc cagctggcca attgccgatc gttggtcaat
     9421 tgttggggcc tactactacg acaccaatgc gaacaagcaa gccgactcta tgttaggtgt
     9481 gcaatacagc tcctgctgct atgcaattcg cgtcggttac gagcggaagc tgaacggttg
     9541 ggataacgat aaacaacatg cggtatatga caacgcaatc ggctttaaca tcgaacttcg
     9601 cggcctgagc tccaactacg gtctgggtac gcaagagatg ctgcgttcga acattctgcc
     9661 gtatcaaaac actttgtgat ttgttgattt accacgtaat ccgcagtgcg gttaattgaa
     9721 atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg atcgcgaata
     9781 ccagtttcgc tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat aacggcgtcg
     9841 tgctggaaag cgacgttgat ggattaatgc aatcggtaaa actgaacgct gctcaggcaa
     9901 ggcagcagct tcctgatgac gcgacgctgc gccaccaaat catggaacgt ttgatcatgg
     9961 atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa aatctccgat gagcagctgg
    10021 atcaggcgat tgctaacatc gcgaaacaga acaacatgac gctggatcag atgcgcagcc
    10081 gtctggctta cgatggtctg aactacaaca cctatcgtaa ccagatccgc aaagagatga
    10141 ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg ccgcaggaag
    10201 tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag ctgaacctga
    10261 gccacatcct gatcccgctg ccggaaaacc cgacctctga tcaagtgaac gaagcggaaa
    10321 gccaggcgcg tgccattgtc gatcaggcgc gtaacggcgc tgatttcggt aagctggcga
    10381 ttgctcattc tgccgaccag caggcgctga acggcggcca gatgggctgg ggccgtattc
    10441 aggagttgcc ggggatcttc gcccaggcat taagcaccgc gaagaaaggc gacattgttg
    10501 gcccgattcg ttccggcgtt ggcttccata ttctgaaagt taacgacctg cgcggcgaaa
    10561 gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa ccgtcgccga
    10621 tcatgactga cgaacaggcc cgcgtgaaac tggaacagat tgctgccgat atcaagagtg
    10681 gtaaaacgac ttttgctgcc gcagcgaaag agttctctca ggatccaggc tctgctaacc
    10741 agggcggtga tctcggttgg gctacaccag atattttcga tccggccttc cgtgacgccc
    10801 tgacccgcct gaacaaaggt caaatgagtg caccggttca ctcttcattc ggctggcatt
    10861 taatcgaact gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag aaagatcgtg
    10921 cataccgcat gctgatgaac cgtaagttct cggaagaagc agcaagctgg atgcaggaac
    10981 aacgtgccag cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc aacgtgttgt
    11041 gatcactccc ggcgagcccg ccgggattgg cccggactta gttgtccagc ttgcacagcg
    11101 tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact ctccttaccg accgggcagc
    11161 gatgctcggt ttgccgctca ccctccgtcc ttattccccc aactcccctg cacaaccgca
    11221 aactgcgggc acattaacgc tacttcctgt cgcgctacgt gaatctgtca ctgcggggca
    11281 gttagcggtt gaaaatgggc attacgtagt ggagacgctg gcgcgagcgt gtgatggctg
    11341 tctgaacggt gaatttgctg cgctgatcac aggccccgtg cataaaggcg tcattaacga
    11401 cgcaggcatt ccgtttaccg gtcataccga gtttttcgaa gagcgttcgc aggcgaaaaa
    11461 agtggtgatg atgctggcga ccgaagaact tcgcgtggcg ctggcaacga cgcatttacc
    11521 gctgcgcgat atcgcagatg ctatcacccc tgcgcttttg cacgaagtga ttgctatttt
    11581 gcatcacgat ttgcggacca aatttggtat tgccgaaccg cgcattctgg tctgcgggct
    11641 gaatccgcac gcgggcgaag gcggtcatat gggtacggaa gagatagaca ccattattcc
    11701 ggtgctcgac aagctgcggg cgcaggggat gaaactcaac gggccgctgc ctgccgatac
    11761 cctgtttcag ccgaaatatc ttgataacgc cgacgcggtg ctggcgatgt accacgatca
    11821 gggtcttccc gtgctaaaat accagggctt cgggcgcggt gtgaacatta cgctgggcct
    11881 gccctttatt cgcacatcag tggaccacgg taccgcgctt gaactggcgg gacgtggcaa
    11941 agccgatgtc ggcagtttta ttacggcgct taatctcgcc atcaaaatga ttgttaacac
    12001 ccaatgaata atcgagtcca ccagggccac ttagcccgta aacgcttcgg gcaaaacttt
    12061 ctcaacgatc agttcgtgat cgacagcatt gtttctgcca ttaacccgca gaagggccag
    12121 gcgatggtcg aaatcggccc cggtctggcg gcattgaccg aaccggtcgg cgaacgtctg
    12181 gataagctca cggtcatcga acttgaccgc gatctggcgg cacgtctgca aacgcatcca
    12241 ttcttaggcc cgaaactgac tatttatcag caggatgcga tgacctttaa ctttggtgaa
    12301 ctggccgaga aaatgggtca gccgctgcgt gttttcggca acctgcctta taacatctcc
    12361 acgccgttga tgttccatct gtttagctat actgatgcca ttgccgacat gcactttatg
    12421 ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga acagcaaggc gtatggtcga
    12481 ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg tgctggaagt accgccgtca
    12541 gcctttacac caccacccaa agtggattcc gccgtcgtgc gcctggttcc tcatgcaacg
    12601 atgcctcacc cggttaaaga tgttcgcgtg ttgagccgca tcaccaccga agcctttaac
    12661 cagcgtcgta aaaccattcg taacagcctc ggcaacctgt ttagcgtcga ggtgttaacg
    12721 ggaatgggga tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca atattgccag
    12781 atggcgaact atctggcgga gaacgcgcct ttgcaggaga gttaacgatg atcaattcgc
    12841 cccgagtgtg tattcaggtt caaagcgtct acattgaggc acaatcttca cctgataatg
    12901 aacgttacgt ttttgcttat accgtaacca tacgcaatct ggggcgagcg ccagtgcagt
    12961 tgttggggcg ttactggctg atcaccaatg gcaatggccg tgaaaccgaa gtccagggcg
    13021 aaggagtggt tggcgtccag ccacttatcg cacctggcga agagtaccag tacaccagcg
    13081 gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg atcgatgaaa
    13141 atggcgtccc tttcagcatc gacattcccg tattccgact cgccgttccc acactcattc
    13201 attaaaagaa tatggcgaca taccttattg gcgacgttca tggttgttac gatgaactga
    13261 tcgcattgct gcataaagta gaatttaccc ctgggaaaga taccctctgg ctgacgggcg
    13321 atctggtcgc gcgcggcccg ggttcgctgg atgttctgcg ctatgtgaaa tccttaggcg
    13381 acagcgtacg tctggtgctg ggcaatcacg atctgcatct gctggcggta tttgccggga
    13441 tcagccgcaa taaaccgaaa gatcgcctga caccgctgct ggaagcgccg gatgccgacg
    13501 agctgcttaa ctggctgcgt cgccagcctc tgctgcaaat cgacgaagag aaaaagctgg
    13561 tgatggccca cgccgggatc acgccacagt gggatctaca gaccgccaaa gagtgcgcgc
    13621 gcgatgtaga agcggtgctg tcgagcgact cctacccctt cttccttgat gccatgtacg
    13681 gcgatatgcc aaataactgg tcaccggaat tgcgggggct gggaagactg cggtttatca
    13741 ccaacgcctt tacccgtatg cgtttttgct tcccgaacgg tcaactggat atgtacagca
    13801 aagaatcgcc ggaagaggcc cctgccccac tgaaaccgtg gtttgcgatt cctggccctg
    13861 tcgctgaaga gtacagcatc gcctttggtc actgggcatc gctggaaggc aaaggtacgc
    13921 cggaaggtat ttacgcgctg gatactggct gctgctgggg tggtacatta acctgcctgc
    13981 gctgggaaga taaacactat tttgtccagc cgtcgaaccg gcataaggat ttgggcgaag
    14041 cggcggcgtc ttaaacacag cctgatacag gaaggccgga taagacgcga ccggcgtcgc
    14101 atccggcact agccgtaaat tctatacaaa cttaccgccg ctccagaatc tcaaagcaat
    14161 agctgtgaga gttctgcgca tcagcgtcgt ggaattcgct gaataccgat tcccagtcat
    14221 ccggctcgta atccgggaaa tgggtgtcgc cttccacttc tgcgtcgata tgcgtcagat
    14281 acagtttttg cgctttgggc aggaactgct cataaacgcg accgccgcca atcaccatga
    14341 tttctggtac gtcaccacac gccgcaatgg cttcatccac cgacttcacc cacgttacgc
    14401 gatcgtccgt acccggttga ctgctgagga taatattttt gcgtcctggc aacggacgac
    14461 cgatggattc ccaggtatgg cggcccataa tcacgggttt atttaaagtg ttgcgtttaa
    14521 accaggcgag atcggcaggc aggttccacg gcatggcgtt ttccatgccg ataacacgat
    14581 ctaccgctaa cgccgcaatc agactgatca ttgagatttc ccgataaaaa aaattgtcgc
    14641 cactatacgt aaagcgtaaa ccgtcgtcga ctggtgcgag gatgatgttg aggaaaattt
    14701 tatattctgc tggcgagtcc acgctctccc actggactcg ccgcattaca atgaaataaa
    14761 aacaattagt tagctgtaaa gtgtgattta cgtcactctt tattaggatg agggtttcgt
    14821 ttccggttca tccgccatgt tgccggtatg tttaccttct tcggttccct gccagccatg
    14881 tcgttgaatt aatgacagat gttcgcggtc ctcggtaatg atctcactta acatcgcgct
    14941 ggtgcgttta tagaccgccg cgcgggcttt ggtgtcgttc tcaaccattg ccatctcttc
    15001 caccatctga atattaaagc ggcggaacac atcggcgcgt tctcgcgctt catacggccc
    15061 cagacctaaa ctttccagtg ccagacgccc ggttttcagc gcaccttcaa aggtttcacg
    15121 ctccggcttt tcaacgcctg cctgacgcaa acgaatgtag tggtcgacat cgcgggcgcg
    15181 ggcaataatc tgcaaatgcg ggaaatgttc tttcaccatc tccgtcagtt gcaggttggt
    15241 ttgcggatcg tcgatagcgt taatcagcac ttccgctttc gccgctccgg cagattccag
    15301 taaatccatc cgcgtggcat cgccataaaa cactttcata ccaaatttac gcaaggtttc
    15361 gatatggtcc ggatcgtgat cgagcaccac cattttcacc ccgctggaga gcagtaaacg
    15421 tccggtgatc tgcccaaaac gaccgaatcc ggcgatgatt acccgcggct gttcttcgtc
    15481 gatctcatcg gcttcacgcg cctcttcact agaagattgc tcaaggcgat tgaggatcac
    15541 cagcagaatc ggcgttgccg ccatcgacag cgccaccgcc agggtcagcg atttcgccca
    15601 ctccggctcc agcacattcg ccatctgcgc cgcaccaaat actacaaagg caaactcact
    15661 accctgccct aatagcaccg caaaccagcg acgctgttta tttggcacct gcaacggccg
    15721 ggcaatcagc cacagcatgg cgattttgat gatgaggaaa ccgagcagca aaatgacaat
    15781 gcgcaacgga ttttcaagca gcgtgccaaa gtctatcgac atgccaacgc cgatgaaaaa
    15841 cagccccagc agcaaacctt taaatggttc gatatcgctt tccagcgcat gacggtattc
    15901 cgagcttgcc agcagtacgc ccgccagaaa cgcgcccatc gccatcgaca agccgacctc
    15961 ttccagcagc aaaccaaagc caaacacgag gaataacgcc acggcactaa acacttcccg
    16021 caagccagag cgggcaacaa aacgcagtgc cggacgcgtg acatagcgcc ccagcaatac
    16081 caccagcacc agcgcacccg ccacttttaa cgccgagaga gcaaatgcgc ccatcgtcgt
    16141 cgaggcactg ctcgccgcca gtagcgggat catcgccacc agcgggatcg ccgcgatatc
    16201 ctggaacagc agcaccgcaa aggcactgcg acccatttgc gtcaccatca gattgcgttc
    16261 attcatcgcc tgcatggcaa tcgccgtaga ggagagcgcc agcgtcatgc cgatcaattc
    16321 cgcgacctgc cagcgcagcc caagcaacat acagaacagc cccagcaggc cgccgcaaat
    16381 caccatctgc aatgcgccgc cgccgaacac cgccgcacgc agcttccaca gcctttgtgg
    16441 atcgagttcg aggccgataa taaacagcat cagcaccacg ccaatctcgg caaagtgcag
    16501 aatagattcg gcatcggtca ccagtcgcag cccccacggg ccaataatgc agccggcgat
    16561 caggtagcca agtaccgctc ccagaccgag acgtaccgca atgggaacaa tcagcgccgc
    16621 cgaaccgaga taaatcagcg cctgaataag cgtatggcta tccatgatgt gcctcctgcc
    16681 attccagcag acgttgctta tagtgccgcg cctgcccttc gagggtttcg tcgtcacaaa
    16741 taaaggtgca gtgcatggca aacggtggca gccagttcag cccgcagtag attgccgtcg
    16801 cctgtagcgg ctgtgacagc acatcaaagc ccggatgcgc accaatctca aaatggcttt
    16861 ccccaccgcc ggtcgtcacc gcccacagta aatgtttgcc atgcagcgcc gtgccgccgt
    16921 gaccgtaagc ccagccgtgc gagaagactt tatcgatcca aagtttaaga agcggaggaa
    16981 tgctgtacca ctgcatcggg tgctgccaga cgatcagatc ggcgcgagac agcgcctcct
    17041 gctcggcggc aatatcgata ttgaagtcag gatagagttg ataaagagag cgaatttcga
    17101 cgccttccag cgtccttgcc tgttcaagca tccgtttatt cgcatgggaa tgatgcggat
    17161 acggatgcgc ataaattata agaatcatag gtagcccgcg tgattacgcc tttgttttcc
    17221 cacaaagtgt agtcatatat tcacgcggct tatagtcaat aatattgatt cagaaaagag
    17281 agaaatctga acaaattacc ccattctctg cccgcggatc gtcatattgc tggtctgcgc
    17341 cagtgacatc cctcgagtct ccggggcaaa cgctacggaa atcaacaagc caaacagcga
    17401 gatacccgcc cccattagca tcgtgttact gataccgtaa ttattgataa agatcggcag
    17461 tgcccaggtc gaaacaatag tgccaatacg gcttaaggac ataatcacgc ccacggcaga
    17521 ggcgcggata tccgtcggga agagttcatt aggatagagc cactgcaaat tacccggccc
    17581 gccagagaaa aaggcataca ccgcaaatgc catcactacc agccagatcc ccatatccgg
    17641 gatcagcccc aaaaccgcca gcgccagcgt catcatggca aagctgccaa tcaacaatgg
    17701 acgccgtccg gcagtgttta gccacagcat cggtggaata cagccgagca taaagaacag
    17761 gctaatcacc acgttcccca gtgccgcgtt tttgccaaca cccaatccca acaaaccaac
    17821 gatttgcggg ccaaaggtgt agatggcgaa catcgggatc acctggcagg tccagatggc
    17881 ggcaacaaac agaacaaaag gaaaatggcg gcgattaaac agatcgcgaa aacgggtttg
    17941 ctgcggctgc tcttcatcga aagccaccgg ttcgccaaac agtttgatca tcatctcttc
    18001 gcactctttt actcgccctt tgcgtaacaa ccagcgggga gattcaggca gttcgaatcg
    18061 accaatcaaa atcaacaaac aggggatcgc tgcgctaccc agcatccagc gccaaccacc
    18121 ttccacatca taaagccagt aaccgaccag atcggcgcag gtcgcgccga cataccacat
    18181 cgcggcaata aagctgatgg aaaacgcccg ctgacgggta ctggagaact cagtgatcat
    18241 cgaagtggcg atgggataat ctgcaccgat gacaatgccg atgagtaccc gcatcactaa
    18301 cagttcgacg ggggatgaga caaacatcgt cgccaccgat atcacgccga tggcgatgat
    18361 atcaatgagg aacattttgc gccgtccgac tttatcggag atatagccaa acagcgatgt
    18421 gccaacgaac agcccggcga gcgttcccgc gcccagcaag ccaatccagt cagcgtccag
    18481 tttcagcgcc ggagtcagtt gctccagcgc cacgccaatc attaccagta cataaccatc
    18541 cagaaacgga ccaccgcttc cccacagcaa aatgcggcgg tgaatagagg agaatttaag
    18601 atcatcaaag tttctggacg gttgcatggg ttattcctgt ttttttgcaa tgagagtcag
    18661 tacggcatta cgtatcaaac caacatcagc cgtaacggaa ctccacacca aaggtgccgc
    18721 gcgggtattc ccactgttcc agcgccgacc ccagccccag aatgcgacag gtgccgcact
    18781 ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc ctgcttcttg tacagacctg
    18841 cggggcacgc tttcaccagc agctccaggg cctgtttatc ggcatcggcc ttcacaacga
    18901 tgtgcggatg ctcttcatcg acattgaatt tattgacgcc cagtttgacg tccacattga
    18961 cgggagaagt cataaaacgg tcactccttt catgccatcc ttgatcagat tgatgaagcc
    19021 cactttcttg ccgtggcgga ggattttctt gcgcatcagt tccggcgcac tgccgtcaat
    19081 ggtgaacagg tcacgcgcca cgcccaccgc cagttccggg tagccgctaa acatgcgtgg
    19141 gttatcaagg aacgccggta gtttctggta catgcgcata tcgcgcagcg ggccactctc
    19201 aagatgctga cgatattccg cgagtttttg cttactgaaa tcgtcgcttt tcatcgctga
    19261 aagcacagtt tttgctgctg cttccccggc ggcaatcgcc agatccatac cgcgaatggt
    19321 aaaaccgagg ttcatacaca ttccggcagc atcaccggca atcaatacgc cgtcaccaac
    19381 caactccggc agcatgttga tacctgcttc cggcactacg tgagcggaat attccaccag
    19441 cttgccgccc gcgatcagcg gtgcaacggc cggatgttgt ttgaaatctt ccagcatttg
    19501 cggcaccgat ttttttgcgt catgcagatg atgcaaacca caaaccagcc ccagcgacag
    19561 ggtgttttca ttggtataaa gaaagccgcc gcccatcagg ccatcagtgg gggaacccgc
    19621 aaacaggcaa gccgcaccct gattaccctg caactgaaaa cggtcttcga taaccgactt
    19681 cggtaattcg atcagttcct tcacgccaac cgccacatcc gtcggtttaa cgcgttttgc
    19741 catccccagc ttttcggcaa ggatggagtt caccccgtca gcaaggatca ccgttttcgc
    19801 ttcaattaca tcgccatcgg cttctacgcc gacaactttg ccatcgcgct gtacgaggtt
    19861 atcgacacga atcccggtaa ttaactgcgc acccgcttct tcggcctgct ccatcagcca
    19921 ggcatcaaat ttactgcgca aaacggagta agaacgctgg gatggtgagg tttcgtcacc
    19981 gttgcagtag tccatagtca tcgcagactt ttccgtcata aacgcgagtt tttcatgggt
    20041 gatcaggcgt tctacggggg cggagtcagc gaaaccagga ataatgtgtt ccagactgtg
    20101 ggcatagaga cgcccgccgg tgacgttctt ggcaccagcg gaattgcctc gctcgataac
    20161 taacacttgc gccccttcgc gggcgagcac cagtgcggca accgaaccgg caagccctgc
    20221 accgacgatg atggcgtcaa agatatcttc ggacataaga gctccctgtc agcggcaggg
    20281 ccaaaatgca gccctgccag agtggatcaa cgcgctaaag ctgcggtcag tgccggaagg
    20341 atcttcacgg cgtcgccaac aatgccgtaa tccgcgtact ggaagatcgg cgcattttta
    20401 tctttgttga tggcgaaaat ggtttgcgac gcattagcgc caaccatgtg ctggatctgc
    20461 ccggagatcc ccaccgccag gtacagttca ggtttcagca tcaggttgga gataccgaca
    20521 tagcgttcgt gctccatcca tttttcgttt tccgccaccg gacgagaaca ggccaactcc
    20581 gcacctatcg ccttgcaaag ctgttctgcc agcgcaatgt tctctttgct gccaataccg
    20641 cgaccaacgc tgaccaccag gcgggctttg tcgagatcga cgctgttgct ctgacgcgcc
    20701 tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc actccacggt gtgcgtttcg
    20761 ccagtgcgtg acgcgtctgg ctgagccaca tcgaacgtgc cgctgctgat ggtcagaacc
    20821 gcatacggcg tggcaatgcg ttcttcgcca atcgccagac cgccgtaaac catgtgtttc
    20881 actgtcgctt taccgtcctg cacgctaaca gcgctggcat cgttagacac cgccgcgtta
    20941 aggcggtaac ccagtttcgc cgccagtaat ttgccgcgac gggtgtttgg caacagcacc
    21001 agaccgtctg cgccgtgctg gcgaatagtg tcagccatga caccggcgta atcttcgatc
    21061 atccgctcgt ccggtttgcc gcttaatttc cagacatgat tagcgccgag ctggattgcc
    21121 tgtgcgccgt cggcatcatt gaggacgaac gtgttgattt gattagctaa agcctgcgca
    21181 ccgttcatca gttccggcag acgagaaggg gtatcgctga atacccagac ttgagaaaac
    21241 gtgttcatag catcccctgt aattaaatga ctttgcgaag attttcagca aatgcggcga
    21301 tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg ctcgcgctgt ttcggcgcgg
    21361 caacctgttg ttctgaccag gctgcctctg cgttaaaacc gatatccgcc gccgaccata
    21421 cctggactgg ctttttcgcc gcgccgagaa tggctttcat cgaaggaatt tgtggggagt
    21481 tgatatcagt ggaaacagca acaaccgcag gcagcggaat gcttaaggtt tctgtttcat
    21541 cttccagttc gcgctcaacg gtgagggtat ctgcagtcag ggagataatt ttgctgacgc
    21601 cgttaactgc cggaatatta aggatttcgc ctaccagcaa accaacctgc tgggcataaa
    21661 ggtcggaaga accatcgcca cagaggatca aatcaaagcc tgctttctga gcggctgcag
    21721 ccagtacgct cgccgtttgt tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa
    21781 tcagttcatc cggaccgcgc gatagcacat ctttacgccc tttggcgttg gtcagggctt
    21841 taccgcccac acttaaggcc gtcacctgcg cctctgctgc ctgttgcttt agctggcaag
    21901 ccgcttcaat agcgttgaga tcgtattggc ttattttggc atcggctttg ctgaagtcta
    21961 acgaaccatc agcattattg accgcaatat cctgttcatc aggcacgcac ttatagcaag
    22021 taataatctt cattgcatct ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt
    22081 taattacaga tatcacgccc tttaatttgg aaataaaact ttaatcac
//
LOCUS       DAZUCC010000065        22051 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZUCC010000065 DAZUCC010000000
VERSION     DAZUCC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.065"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..773)
                     /locus_tag="ACHY09_002162"
     CDS             complement(<1..773)
                     /locus_tag="ACHY09_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFS3885132.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPYRILTGLVDRFGR
                     TQTFHREAGGEFSGEITGVTDGAGRHFRLVLTTQALRAE"
     gene            complement(976..1377)
                     /gene="nikR"
                     /locus_tag="ACHY09_002163"
     CDS             complement(976..1377)
                     /gene="nikR"
                     /locus_tag="ACHY09_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFS3885133.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1382..2188)
                     /gene="nikE"
                     /locus_tag="ACHY09_002164"
     CDS             complement(1382..2188)
                     /gene="nikE"
                     /locus_tag="ACHY09_002164"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFS3885134.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(2185..2949)
                     /gene="nikD"
                     /locus_tag="ACHY09_002165"
     CDS             complement(2185..2949)
                     /gene="nikD"
                     /locus_tag="ACHY09_002165"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFS3885135.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENTARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2949..3782)
                     /gene="nikC"
                     /locus_tag="ACHY09_002166"
     CDS             complement(2949..3782)
                     /gene="nikC"
                     /locus_tag="ACHY09_002166"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFS3885136.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3779..4723)
                     /gene="nikB"
                     /locus_tag="ACHY09_002167"
     CDS             complement(3779..4723)
                     /gene="nikB"
                     /locus_tag="ACHY09_002167"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFS3885137.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4723..6297)
                     /gene="nikA"
                     /locus_tag="ACHY09_002168"
     CDS             complement(4723..6297)
                     /gene="nikA"
                     /locus_tag="ACHY09_002168"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3885138.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6408..6995)
                     /gene="acpT"
                     /locus_tag="ACHY09_002169"
     CDS             complement(6408..6995)
                     /gene="acpT"
                     /locus_tag="ACHY09_002169"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFS3885139.1"
                     /translation="MYRIVLGKVSTLSAAPLPPALREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(7050..8099)
                     /locus_tag="ACHY09_002170"
     CDS             complement(7050..8099)
                     /locus_tag="ACHY09_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS3885140.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            8231..9448
                     /locus_tag="ACHY09_002171"
     CDS             8231..9448
                     /locus_tag="ACHY09_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3885141.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9452..10009)
                     /gene="dcrB"
                     /locus_tag="ACHY09_002172"
     CDS             complement(9452..10009)
                     /gene="dcrB"
                     /locus_tag="ACHY09_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFS3885142.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(10082..10747)
                     /locus_tag="ACHY09_002173"
     CDS             complement(10082..10747)
                     /locus_tag="ACHY09_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFS3885143.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10969..11214
                     /gene="tusA"
                     /locus_tag="ACHY09_002174"
     CDS             10969..11214
                     /gene="tusA"
                     /locus_tag="ACHY09_002174"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFS3885144.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDELPYRYLIRKGG"
     gene            complement(11316..13514)
                     /gene="zntA"
                     /locus_tag="ACHY09_002175"
     CDS             complement(11316..13514)
                     /gene="zntA"
                     /locus_tag="ACHY09_002175"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFS3885145.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVMLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13588..14214)
                     /locus_tag="ACHY09_002176"
     CDS             complement(13588..14214)
                     /locus_tag="ACHY09_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFS3885146.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14355..14714
                     /locus_tag="ACHY09_002177"
     CDS             14355..14714
                     /locus_tag="ACHY09_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFS3885147.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDI
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14717..14986)
                     /locus_tag="ACHY09_002178"
     CDS             complement(14717..14986)
                     /locus_tag="ACHY09_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFS3885148.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14976..15572)
                     /gene="rsmD"
                     /locus_tag="ACHY09_002179"
     CDS             complement(14976..15572)
                     /gene="rsmD"
                     /locus_tag="ACHY09_002179"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFS3885149.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15722..17218
                     /gene="ftsY"
                     /locus_tag="ACHY09_002180"
     CDS             15722..17218
                     /gene="ftsY"
                     /locus_tag="ACHY09_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS3885150.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPELVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            17221..17889
                     /gene="ftsE"
                     /locus_tag="ACHY09_002181"
     CDS             17221..17889
                     /gene="ftsE"
                     /locus_tag="ACHY09_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS3885151.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17882..18940
                     /gene="ftsX"
                     /locus_tag="ACHY09_002182"
     CDS             17882..18940
                     /gene="ftsX"
                     /locus_tag="ACHY09_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS3885152.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            19185..20039
                     /gene="rpoH"
                     /locus_tag="ACHY09_002183"
     CDS             19185..20039
                     /gene="rpoH"
                     /locus_tag="ACHY09_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFS3885153.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            20311..21414
                     /gene="livJ"
                     /locus_tag="ACHY09_002184"
     CDS             20311..21414
                     /gene="livJ"
                     /locus_tag="ACHY09_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFS3885154.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQVGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21602..21985)
                     /gene="panM"
                     /locus_tag="ACHY09_002185"
     CDS             complement(21602..21985)
                     /gene="panM"
                     /locus_tag="ACHY09_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFS3885155.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5211 a         5855 c         6125 g         4860 t
ORIGIN      
        1 tcttctgccc gcagcgcctg cgtggtcagt accagccgga agtgacggcc tgcgccgtcg
       61 gtcacgccgg tgatttcgcc gctgaattcg ccgccagcct cacggtgaaa cgtctgtgtg
      121 cgcccgaagc ggtccaccag cccggtcagt atccggtacg gcggcagcgg cgcaggcagc
      181 acctcatccg cctccggcac ccgctcacac cagccgagta cccaccacgg cccctgcgga
      241 ctgttcgtcg ccagataacg atgcggactc aggcgaagct cttccggcag cgcctgccag
      301 agtgcggcca gccggtgacc ttcatccagt ttcgccacgc cgccgcgcac cagccagagt
      361 gactcgctgc ggctgtaacc gtcctcaccg ggaaacaggt gctcaaaata caggcttctg
      421 ccgccgttat cactgagtat cagtgtgtta tcgcgcagct gtaagcggat atccgcaggc
      481 attttccagc cggggccgag gctccccacc ggcgcgggcg tttttgtccg gtaactgctg
      541 taggtgcggg agagaatgaa cggcagcggg ccgggcaggg caaggtcggt ttcgccggga
      601 aggaccttcg caccgagcag cggattgacc ggatgaccgg acgtcacccc gccggggcac
      661 accgaacagg ccacaccggt gggggcacca atacgtaccc cggcagaacc ctgaacaatg
      721 ctaccgccat actgcgtcat gtcgccctga cgcgccgccg gttttccgct catcgtaaaa
      781 tccttttcta ttattttaat gacagtatct tcatacgaaa aagcgaagaa aaacttgtgg
      841 tcacaatgaa ttgaagattt ttaggaatat atttagaacg ttacaaaaga gaaggttcgg
      901 atagggtatt tagagaggaa tcaggtgtgc tagtggccct ttgttgatcg ttcaccaaag
      961 gacccaataa taggcttaat cttccttcgg caagcactgc aaatgcccgt gccgcacgcc
     1021 gcgctgggcg ataacgtcat cggcaaaatg ctgcacgtcg cccatatcgc ctttcagcac
     1081 ggcgatttcc agacagtcgt cgtgattgat gtggacatgt agcgtcgcga cggagagatc
     1141 gtggtgatgg tgctgggtgg agacaatacg gctggctaag tcgcgttttt cgtgttcata
     1201 cacataggag agcaccgcga aaccttgcgt gccgtgctgc tgggtagctt cctgcgccag
     1261 ggcgctacgc agaatatcgc ggatagcttc ggaacggttg ttatagccac gacgctggct
     1321 caggctgtcc agcgtctcca gtaaatcgtc atcaagcgtg atggtaactc gttgcataga
     1381 cttaaacctt ttctgtggtg cgacggcgca cggggaatgc gggtaatacc gcgttttgta
     1441 gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc caccacctgg gtttcgacga
     1501 tttgtccgtt gtccatcacc atcacccgct gacagaagcg ttccaccagg cgtaagtcgt
     1561 gggtgatgaa caggcaggcg gtgccaaact gttgttgcag ctttttcagc aggcgaatga
     1621 cgcccgcctg taacacgaga tcaaggttag aaacggcttc atccagaatc agtagtttcg
     1681 gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag ctgaccgccg cttaactgcg
     1741 gtgggcgttt gtctagaacg ctgtcatcga gatcgaccgc cttcagcatt tcgctggcac
     1801 gcgccagttg ttcggctttt ttcagcgata gcaggtggcg catcggttca cgcaggatct
     1861 cgcgcacggt ttttcgcgga ttcacggcgc tgatggagtc ctgaaatacc atctgaatat
     1921 cgcggcggaa cgctttacgc tgggcgcgat tgagctttgc caacggttcg ccgcgccagc
     1981 taatattccc ttgtgaggga gactctaagc ctaccagcag ccgcgcgaga gtgctttttc
     2041 cgcagccgct gcgccccagc agagcgacgg tttcgccgct tttcagggtc agggaaacgt
     2101 tattcagtac cgcctgatgc tgatgttttc cgctaaatcc accgtgcgca tagtgatgtg
     2161 aaaggtcgca gacgttaagt aaagtcatga tgccagctcc ataccgtaga gggcgagatg
     2221 agcggaaacc aggctgcgcg tcaccgtatg tttgggggcg ttaaacagcg tttctacatc
     2281 gccctgttcg acaattttac cgtgtgacat caccgccacg tcatccgcca gacgcgccac
     2341 aacgcccata tcatgggtga ccagcagcat tcccggtgct tgtttttgca taatgctttc
     2401 cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg tcggtggtcg gttcatcggc
     2461 gatgataaac ggcgattcac acaacaccgc catcgcaatc atcatgcgct gcaacatgcc
     2521 gccgctcatc tcgaacgggt acagcttcaa cacgcgcgcg gtgttttcca accccaccgc
     2581 ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc cccagcgcca ggcaggtttc
     2641 acgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg ctgcgcgggt tctgcatgat
     2701 ggtggcaatt ttgatgccgc gcagggcaca gggagaaacc ggtttgccat cggctaaaat
     2761 ttcccccgcc gtctggcgaa cgcctgcggg cagaatgccc agcgtcgcgg cgcaggtcag
     2821 tgatttcccg ctgccgctac cgccgactaa cgccagcaca cgcccgcgtt taagggttaa
     2881 cgatacgccg tgcaccagcg gttgtgcggc ctgtagcgcg atattacgta gttcaatctg
     2941 ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat ccagatgatc gcgcagtgcg
     3001 tcacccacca ggttaaaggc catcacgctg ataaacagcg ccagccccgg ccagaacatt
     3061 tgcagcggct gggtccagat atactgacgc gcgtcgttaa tcatcacgcc ccattcggcg
     3121 gtcggcgcgg tcacgccgag accgaggaag gacatccccg cgacgtgtag catcatatga
     3181 ccgatatcca gcgtcgccag caccagcagc gaggggatca ccgcacctgc cagatgatca
     3241 ataaacaccc gcacatggcc cgcgccagaa agccgtgatg ccagcacaaa ctcgcgctgg
     3301 cgcagtgaaa tcaccaggct acgcaccatg cgtgcatacc acgcccagtg cgacagggcg
     3361 atggctataa tcacattggt caacccggtg ccgagcacgc cgaccataaa gaacgacaga
     3421 atcgaggttg ggaaggtcat aaacatatca gcgacgcgca tggtggcctg atcaacgcgc
     3481 ccgccaatca gcccggcgct gccgccaata atcagcccta atgtcagcac caacagcagg
     3541 caggccatta ccgagccgag cgacacgcgg gtcgccgcca tcagccgcga gaaaatatcg
     3601 cgccctaaat gatcggtacc cagccagtgc tgcgcatccg gtgaaagcag gcgcgacggc
     3661 agatcaatcg cctgggggtc atacggcagc caccactggc tggtgagcgc aatcagcgcc
     3721 agcagggcga taatgatcag cgccaggcgt accgaccagc gggaagagag gaaaaagttc
     3781 acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag cgcggcgttg agtaaatcaa
     3841 caatcaaatt acagaccaca aaaaccacca ccatcatcag cgtaaagcac tggatcaccg
     3901 gatagtcacg gttaaaaatc gccgacaccg catagcgccc gacgcccggc caggcaaaga
     3961 tgttttcgat aatcatcgtc ccgccaatca gttcgccaat gtgcatcccc acggcggtga
     4021 tcatcggcag cgaggcattg cgcagaatat ggcggcgttc ggtctgtttg tcgttcaggc
     4081 cgcgtagccg agcccaggtg acgtgacgct gaccggcgac gtccagcata ctggcgcgca
     4141 gcagccgcgc gttaatcgcc agcgacataa aggcaatgga aaccgcaggc aaaatgatgt
     4201 gctgccagcc gccgtagccc atcgcgggta gccattgcag atataccgaa aacgccatca
     4261 ccagcaaaaa cgccagccag aagttgggca tcgacacgcc gagaaacgcg atgaaacgta
     4321 cggcgaaatc cggcagacga tcgcgatggc gcgccgccca gataccgagc ggtacggaag
     4381 tgagcagaat taacaccagc gcggcacctg caagttccag cgtggcgggc aggaagttca
     4441 gcatatcgtc cagcaccggg cgttggctgg cgaatgagat accaaagtca agatgcagcg
     4501 ccttccacaa ccaggtaccg tactggacgt acagcggctg atccagcccc agcatggtgc
     4561 gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga taaacgcaga taatcgagcg
     4621 ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat caccgaggcg gcgagcacca
     4681 tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa cattaaggtt tcaccggttt
     4741 aatctgttcg aacggaattt cggtggcgat cggcgcgtag gggatgttac ccagctccgg
     4801 ttttgatacc accatcattg agatgtaact gataggcaga taaaccgcct cgtcatgcag
     4861 acgggtcaga atgtcgcgat acagcgcctg acgttgcgtt tcgtcatggg tcgccagcac
     4921 ttcgccgatc tctttatcaa tcagcggctt gtcggctaaa ccttgctgtg cctggaagtc
     4981 agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt ggatcgtatg gcgcgcccca
     5041 ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga cgagcataga tactgctctc
     5101 ttcttcgcca atcagcgaga catctgcgcc aatctggcgc atatcagcct gaatgatttc
     5161 cgccatcgat ttgcttaacg catcggtgcc gatgaacgac agttcaatgc gcagcggctg
     5221 accatttttc tcgcggatgt ctttgcctgc aggcagcgtc caaccggctt tttccagcaa
     5281 cgctttcgct ttctgcggat cgtactggcg cggtttcagg ccgaggttgg cgtagggcac
     5341 agaaggggca aacagggtgt cggcgacctg ctgggtacca tacaacgcgt tatcaatcag
     5401 cgattttttg ttcaccgcgt aattaagagc ttcacgtact gccagctcgt tggtgggggc
     5461 tttggcggta ttgagcgcca gcatcacggt ttcgatcggc tgtgacagtt gagtgtggta
     5521 agccgggttc tggctaaagc gggcgaaggt atcgagcggt aataaccctt cgtttccgta
     5581 cagcaggtcg atatcgccag tttcaaacgc caccgcacgg gtagtcgggt ccgggatcac
     5641 gttaaaggtg atctttttaa tcgctggctt ttcgccccag tagttttcgt tacggacgaa
     5701 gacatcgtac tgattcagtt tcgattcctg taaaacccac ggcccggtgc caatcggcgc
     5761 tttaattccg ttcatggttt cgtggttttt aaactgcgag ggtgcgataa aacggaaagg
     5821 acggggcagg gccagttctt gcaggaaagg atagtaggcg cttttcaggg tgatttgcag
     5881 ctcgttttta ctgagtgctt tgacatcaac aatctggttt gccagctcca gccaggcgtg
     5941 acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc gccgcctctg catcaaacgg
     6001 ttcgccgttg gagaatttca cgtcatcacg cagggtgaag gtccaggttt taccatcttc
     6061 tgaatgagtc cagctttttg ccagccacgg gatcaccgaa ccgtctgcct gatatttcac
     6121 caatggttca taaaccatgc tctgggcgaa catctggtta ggcgtgtaaa ggtgcgggtt
     6181 tagtggcccg acattcaccg gccaggcggt ggtgatttca tctggtgcag cggcatggac
     6241 gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg cggagtgtgg agagcatggt
     6301 aaccccaatg gattaaaata gatggcggaa ataagtatga cgattttaag tattcgtcat
     6361 actgattacc tgttctggat caatagatgg gcagtcgggt gggcggatca gttaactgaa
     6421 tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg taggggtgca gatcgccagg
     6481 cttaaattgt ctagctgaca atggctgact gacagtgatg agtgataggt gctgtctacg
     6541 ctgacgattt gccaggcgct gccgccacgc tgtttaacga tggcttcttt gcgcgtccag
     6601 atgcgccaga acatttccag ttgctgatca ggatgcacgg cgtccatctc ggcatgttcc
     6661 ccaaggctga atacggcgtt cgccagccag cgccagttgg cgcgcgggcg aatcacttcg
     6721 atatcgcagc caacttcgcc ttcatcgctc aacagcaggg cgatatcgtc accgctatgg
     6781 cttaagttga accatagcgg cgtttccggc gcgaatgccg gtttgccttg ttcgccatag
     6841 atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg cacgccccgc cagccagcgt
     6901 tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaagccg gtggcagtgg agctgcgctt
     6961 aaggtcgaaa ctttccccag aactatccga tacatatcag ggccaacgtt taatggaaaa
     7021 tgaaagtgcg tatcgtaaca cttgtcgcct catcccggta accgactttt cggtctgcct
     7081 ggccccagta aaatcgccag tttgctaccg cctttggtgg tttccatcca gattttacac
     7141 acgctggtta atggcaccga aagtagcatc cctaccgggc cgagcagcca tccccaaatt
     7201 aacaatgaaa gaaataccac catggtggac atccccagac gatggcccat catccgtggt
     7261 tctaaaatat tgccgatgac catatggacc actaaaaaca atgcgccgac cagaatacat
     7321 tcgtaaatac cattaaacag cagcacctga atcattggcg gtacggcgga aattaccgca
     7381 ccgatattgg gcacgtagtt gagcaaaaac gccagtaccg cccacatcag cgcaaactgt
     7441 acgcccataa gttccagccc cagccagacg ataacgcctg tccataaact tagcagtgtt
     7501 ttcaatgcca gataatgcga aacgccttta agtgcgcggt gtagtccggc gatgtgaatt
     7561 tgtggattat tcaacgcgaa acgcattttg taagggacgt ggcgcacttc aaacagcata
     7621 aaaactacgg tcatcaccag caaaagcacg ctcgccattg ccccggaaag cccggtcatt
     7681 agcgctgtgg tgaaggtaac cactttttcc gagtccatcc gctgcagcat tcgctccggt
     7741 gacatatgca aattaagaaa aggcaacatc tcctgcaatt taaaaagttt gcgcgtcagc
     7801 tccttattaa acttcggcag catagagata aattcgttaa acgatgccgc cagtacgccg
     7861 accagcgcag ttagtgcgat cagcatcacc accaccacaa tcgtaatggc aacggggcgt
     7921 tgtactcccc gacgaataaa ccaggtgacg agcgggttga ggacgatggc aaaaaacagc
     7981 gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa tgcccgcgag gatcactacc
     8041 agcgaggcca gcttgagcag aatgtgcatg cccgttttat cgggttgagg ggtttccatt
     8101 ggggcttcct tgtgactttt tgtattaagt gtagcgggag tcacgccagc aatattctga
     8161 ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg aaacactgtt gtaaaaatgt
     8221 ggtgatcctc atgcccgaac ccgtagccga acccgcgcta aacggattgc gcctgaattt
     8281 gcgcattgtc tccattgtca tgtttaactt cgccagctac ctcaccatcg ggttgccgct
     8341 cgctgtatta ccgggctatg tccatgatgt gatgggcttt agcgcttttt gggcaggatt
     8401 ggttatcagc ctgcaatatt tcgccacctt gctgagccgc cctcatgccg gacgttacgc
     8461 cgatttgctg ggacccaaaa agattgtcgt cttcggttta tgcggctgct ttttgagcgg
     8521 tctgggatat ctgacggcag gattaaccgc cagtctgccc gtcatcagcc tgttattact
     8581 gtgcctgggg cgcgtcatcc ttgggattgg gcaaagtttt gccggaacgg gatcgaccct
     8641 gtggggtgtt ggcgtggttg gctcgctgca tatcgggcgg gtgatttcgt ggaacggcat
     8701 tgtcacttac ggggcgatgg cgatgggtgc gccgttaggt gtcgtgtttt atcactgggg
     8761 cggcttgcag gcgttagcgt taatcattat gggcgtggcg ctggtggcca ttttgttggc
     8821 gatcccgcgt ccgacggtaa aagccagtaa aggcaaaccg ctgccgtttc gcgcggtgct
     8881 tgggcgcgtc tggctgtacg gtatggcgct ggcactggct tccgccggat ttggcgttat
     8941 cgccaccttt atcacgctgt tttatgacgc taaaggttgg gacggtgcgg ctttcgcgct
     9001 gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta ttccctaacg gcattaaccg
     9061 tatcggcggc ttaaacgtag cgatgatttg ctttagcgtt gagataatcg gcctgctact
     9121 ggttggcgtg gcgactatgc cgtggatggc gaaaatcggc gtcttactgg cgggggcagg
     9181 gttttcgctg gtgttcccgg cattgggcgt agtggcggta aaagcggttc cacagcaaaa
     9241 tcagggggcg gcgctggcaa cttacaccgt atttatggat ttatcgcttg gcgtgaccgg
     9301 accactggct gggctggtga tgagttgggc gggcgtcccg gtgatttatc tggcggcggc
     9361 gggactggtc gcaatcgcgt tattactgac gtggcgatta aaaaaacggc ctccggaaca
     9421 cgtccctgag gccgcctcat catcttaaaa cttactgaat aaccagcgta ttaatgatgt
     9481 tttctgcggt ggtctgcgct ttttgctgat tgtcagctgg cagcgtaatt tgcatggtca
     9541 gcagttgatt acccacgtta cccagaataa cggaagagta cgccgtctgg cctttcgcgg
     9601 agataatgct gtctaactgc tgcattttgt gacctttcag ctcaatggct ttattggtta
     9661 ccacttgcag ctgcggatcg cggctacgtt gctgatcttc cagacgcttc gccaacaccg
     9721 ccagatcttc tttcggatca tcgcccatga tgacgatgac tgctttttgc ccagtggcgt
     9781 cggaccagac atgcatgtta ttggcctgcg ttcccagctt accgctctgg tcggtcatat
     9841 ccgctggcag cgagaaactt aacttgccat caagcaggtt gacgggattc ccggtagcgt
     9901 tactttccgc gaccgacccc tgcgccgtag cgttagtgtc tttatcatca caggccgcaa
     9961 gccccataac cagcaggcca attccgacat atttaaccag attgcgcatt gacttcttcc
    10021 tttcgataaa cggccataac ggctcattca tccatcttat cacaactctg ataacgaacc
    10081 tttaactcgc ctgcaaagcg ttgatttcag atttatctgc cagtcttttc aacagcatat
    10141 tgagtaatac gccatacatt ggcaggaaga aaacgatact gattaacact ttgaaacagt
    10201 aatcgaccag cgcgatttcc atccagtgtt cagccataaa ggcatccggg ctacgccaga
    10261 aggcaatgaa gaagaaggcc agcgtgtcgc tgacgttacc gaacagtgtg gacgctgtcg
    10321 gtgccagcca ccagtgacga ctctgacgca ggcggttaaa aacgtgcacg tcgaggatct
    10381 gccccagcgc gtaggccatg aaactggcgg tggcgatacg ggcgacaaac aggttgaagt
    10441 gggcgagcgc gccgaatccc tgccaggaac ccatatagaa cagcgacgag atgacgtagg
    10501 agatcagtaa cgccgggatc atcaccgcaa agatgatacg acgggccagc ggtgcgccaa
    10561 aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg aaagctaaat gcgccccagg
    10621 tggtatggaa accaaaaatg gagacgggaa gctgaaccag atagttactg gaggtgatca
    10681 ccagcagatg aaataacgat aaccagaaca acgccttata gcgttgagtt tgcgagaaaa
    10741 cgttcatatt gtaccttttt gattaaccat tggggtgagg gaacccaata cgtacgacac
    10801 gctattttac cttgattaaa taacgttctt accctttttt cgagccgccg catgatactg
    10861 ctttacgttg acaatgcaat ggttaatttt cacgcaatcg ttaacctggt ttgcttacgg
    10921 actcacaggg cgtaaactag cgccgttttt ttatgtgatg agaagaaaat gaccgatctc
    10981 ttttccagcc ctgaccacac actcgacgca cttggcctgc gctgcccgga accagtaatg
    11041 atggtgcgca aaacagtgcg caatatgcag ccaggcgaaa cgttgctgat tatcgccgac
    11101 gatccggcca ctacccgcga tattcctggg ttttgtacct ttatggaaca cgaactggtt
    11161 gctaaagaga cggatgaact gccttatcgt tatttgattc gcaaaggcgg ttgatgggga
    11221 aagattggct tcgatgccgc cttttcccct caccctaacc ctctccccag aggagcgagg
    11281 ggaccgatcg cgctcaatgt tgcgatcggt ttgccttatc tcctgcgcaa caatcttaac
    11341 gcattcgctg tcaccagcac cgtcgcaccc gtatctgcca gcaccgccag ccatagcccg
    11401 gtcattccta acagtgtggt gacgaggaag atccctttca gccccagcgc aatagtgatg
    11461 ttctggcgga tattggcgtg agtggcgcgt gccagttcaa tcatttgcac caggccgcgc
    11521 aggtggttat gggttaatgc tgcatcggcg gtttccagcg ccacgtctgt gccactgccc
    11581 attgcaatcc cgatggcggc ggctttcatc gctggcgcgt cgttaatacc gtcaccgacc
    11641 atcgccagcg gcgcatgttg attcagctcg gtcaccgctt tgactttatc ttccggcaac
    11701 aggcccgctt taaactctag ccccagttcc ccggcgattg ccgccgctgc gcgtggatta
    11761 tcgccggtga ggatcacccc tttgacgccc agcgcgttca gttcactgat ggcagttgca
    11821 gcatcggcgc gcagggtatc ctgtaacgca atgacaccaa gcacgtcatc gttacgtact
    11881 accagcacta ccgtttgccc ggcgctttcc agttcgttaa ttaaaccagc aaatgcatca
    11941 gcgggatgtt tcccggcagc gcatatcaac acgcgctcac cgttaacctg cgcttcaatg
    12001 ccagacccaa ccagcgcccg ctgtgattgg gcggcgggaa tggcgagttc agcaacctgt
    12061 gcttctcgca cgatggcttg cgccagtgga tgcgtcgcgc cttgctcgac cgccgctgcc
    12121 agtgtcagca gttcggattc actaataccc gttgccggat gaatcgcggt aacgcgcggt
    12181 ttaccgacgg tcagcgtacc ggttttatca aacgccacct gggtaacacg acccagttgt
    12241 tccagcgccg ctccgccttt aattaacgcc ccacgacgcg ctgctgctgc cagcccggag
    12301 gtaatcgccg caggcgttga gataactaac gcacacgggc agccaatcag cagcagcgtc
    12361 agccctttat aaatccactc ttgccagctg gcggcaaaca gcagtggtgg caccagcgtt
    12421 accagcagag cgacggccat aatcgctggc gtgtaaatac ggctgaaacg gtcgataaac
    12481 cgctcaatgg gagcgcgacg ctcttcggct tcttcgatca gtttcagaat ccggtcaatg
    12541 gcgctggcac ccggttctga cagcacttcc agcattacca gacggtctac gctggtggcg
    12601 cctgcaggaa ctttatcgcc cgtcgcgcgc tccacaggaa tggattctcc ggtcagggcg
    12661 ctttcatcaa aactggcaaa cggtgagagc agtttaccgt cggcaggcaa acgcccacct
    12721 gccgcaactt caatcacatc gccaggccgc aggctgttta ttgccacctc ttcacgctca
    12781 ccgttacgca gacgcgtggc ggtttctggc ttcagcgcca ttaacgcgct gaccccttga
    12841 cgcgcgcgac tggcggccca gccctccagt cgttcgccaa tcaaaaacag cagcaacacc
    12901 atcgcagctt cagccgttgc gccaataaac agcgcaccaa tagcggctac gctcattaac
    12961 gtttcaatgg cgaagtagct gccggatttg atcagccgta atgcctgacg agcaatcggg
    13021 tacagcccaa ccagcgtggt cgcgataaac gccagttgcc cgaacggatg attgaactgc
    13081 tccagacccc agctgattgc catcatcacg attagcgtaa tcagcggcag attctctttc
    13141 aggcgtgatg cttgtggttc gtcggcggac tgttcatcgc gcagggaata gcctgctttt
    13201 tgcaccgcag attcaacctg ggcacggatg tcattgtcgg catcgaccac cagtttttcg
    13261 gtggcgaaca acacctgcac ctgattcacg cctgcaagct ggcgcacggc attttctacc
    13321 ttgcgcgcac aggcggcgca gtccatgccg ctgactttcc agctatagcg ggtgccggag
    13381 acgttttcag agagagttgg cgtgctggaa catgcgccgt cgcagcaaca gtcgttggcg
    13441 ttctgtaccg tggttagcgg tttgaacgca gcaaattgag gggctttctt gccgtgattg
    13501 tcaggagtcg acatggcatc ctccggttaa gttttttctc attaaccgaa ggatacactc
    13561 tggagtcgac tccagagtca agttttatca gagatacagc gagcggacga tgaggaaatg
    13621 accggcaaag tagcaggccg cagcaatcgc gttatctgcc cggaagcggc ggcgatagtg
    13681 gctacccagc cagacaaagt tactgataaa cagcaacgaa gcgccgacaa acgcagagag
    13741 cgccggagct gtcggacgga agaaccacag ttcacctgcc agccacacca tcaccagcgt
    13801 catgccgata aacgtgcaga taggccaacg atactcttcc agacgcgtcc agataatcgc
    13861 cagtaacagc gcacccagaa ccagcaacac cagtggcaga ggccagaaga aagagagcgt
    13921 catctgactg gcaaaataga tggtgtacag caggtgcgag aggaaaaacg cgccgatggc
    13981 gtacatcaga cgttgacgtg gcaacagggt tagcgcatcg cccagcagtg aggcgcacag
    14041 ccctgccagc accagatagc taatggcgtc gaacatcggc gcttgccagg ccagtaacag
    14101 caggagaaga agggttaacg gtttaaacac ccagcgttgc caggttggcc cacgatacga
    14161 tgcatccaca gatagccatg cggaaagaca gacagcgata aacgaccaaa gcatcttagt
    14221 tccttgaatt cgttattttt tcgtaagcgg aatgcctacg gtcttgcttc cagtttagtg
    14281 gcgagggtgg acggatgaca acaccataac tggcagggta tgcttatttc cgcattttcc
    14341 gatgagggat aaagatgagc aaaccacctc ttttctttat tgttatcatt ggcttaattg
    14401 tcgtcgccgc atcgtttcgt tttatgcaac agcgacggga aaaagctgat aatgatatgg
    14461 ctccgctcca gcaaaagctg gtggtggtga gcaacaagcg ggaaaaaccg attaacgatc
    14521 gccgttcgcg ccagcaggaa gtgactccgg caggcaccag tatgcgctac gaggcaagct
    14581 tcaaaccgca aagcggagga atggagcaga cgtttcgcct cgacgctcag cagtaccatg
    14641 ccctgacagt gggcgatata ggtacgctga gctataaagg aacgcgcttt gttagctttg
    14701 taggcgaaca ataaggttat tttttaactt taaatttctt ctgccagacc agcagttcaa
    14761 acacgccgaa aaggaaaatc cgcacctttt cggcggtggt catctgcggg ccatctttag
    14821 gtaaagtgga tttcaatagc gccagctgca taccatgcat cagcacggta aaaatcagcg
    14881 ccacgttaac gaaaatattc agcgggcgcg ggaagggatg caccagattg aggattaaaa
    14941 atccccaaac gcagagcatt aacaaacgac caatattaat cagcatcact ttctccttgt
    15001 gcttcgcgtt gatacagccg ataagccacc tgacccgcca ctttttcccg atgtaatgac
    15061 cagtttgccg gaacagtggg cagaccgttt tcgacttcgc tttcgacata aatcagggct
    15121 tcgtcagcca gccagccgtt atcttccagt aaatttatcg tctcttctaa caagccacgg
    15181 cggaacggtg gatcgacaaa cacgatatta tgcggtgtgc ctttttgcgc caggaatgac
    15241 attgcgttgc tgttcaccac gcgtgcattg cctgctttta gtgtcgccag attcttaatt
    15301 aactgctgag aaaccgcgcg atccatctca atcaacgtgg cccccgcagc atagcgcgac
    15361 aacgcttcca gccccagcgc gccgctcccg gcgaagcaat ccagacattg ggcgtcaaca
    15421 atgaccggag ccagccagtt aaacaacgtt tcgcgtacgc ggtcggtggt ggggcgcaga
    15481 cctgggctat caggaaccgg gagtttacgg cctcgccact gcccgccaat aatgcggatt
    15541 tggccgctgc cggaatgatt cggttttttc atgataaatt gctcaatccg ccagataaca
    15601 catacttgca ggcggtgatg tgaattaagt taagtgatag actatttcat cattttttta
    15661 gctgctatgt acatagcgtt aacgctgtgc catgaagcaa cagcgaggag tgtagtcgca
    15721 aatggcgaaa gaaaaaaaac gtggcttttt ttcctggctg ggctttggtc aaaaagagca
    15781 gaccccggaa aaagagacag aagttcagaa tgaacaaccg gttgtagaag aaatcgttca
    15841 ggcgcaagag cctgcgaagg cttcggaaca agccgttgaa gagcagccgc aggcgcatac
    15901 tgaggccgaa gcggaaactt ttgctgccga cgttgtggaa gtcactgaac aggttgttga
    15961 aagtgaaaaa gcgcaacctg aagcggaagt tgttgcacag ccggaactgg tcgtagaaga
    16021 aacgccggag ccagtggcta tcgagcgtga agagctgccg ttgccggaag acgtcaacgc
    16081 cgaagaggtt tcgccagaag agtggcaggc cgaagcggaa accgtagaga ttgtcgaagc
    16141 ggcggaagaa gaagcggcta aagaagaaat caccgacgaa gagctggaag cacaggcgct
    16201 ggctgccgaa gcagcagaag aggcggtgat ggtggttcct ccggcagaag aagagcagcc
    16261 ggtagaagaa atcgctcagg agcaggaaaa accgaccaaa gaaggtttct tcgcgcgcct
    16321 gaaacgcagc ctgttaaaaa ctaaagaaaa tctcggttcc ggatttatca gcctgttccg
    16381 cggtaaaaaa atcgacgatg atctgtttga agagctggaa gaacagctgt tgatcgccga
    16441 tgtgggcgtg gaaaccacac gtaaaataat caccaatctg acggaaggcg catcccgcaa
    16501 gcagcttcgt gatgccgagg cgctttatgg cctgctgaaa gaagagatgg gcgagattct
    16561 ggcgaaagtc gatgagccgc tgaatgttga aggtaaaacg ccgttcgtga tcctgatggt
    16621 gggcgtcaac ggtgtgggta aaaccacgac gattggtaag ctggcacgtc agtttgagca
    16681 gcagggtaaa tcggtgatgc tggcggcggg cgatactttc cgtgcggcag cggttgaaca
    16741 gcttcaggtc tggggtcagc gcaacaatat tccggtgatt gcccagcata ccggggcgga
    16801 ttccgcctct gttatcttcg acgccattca ggcagctaaa gcgcgtaata tcgacgtcct
    16861 gattgccgat accgccggac gcctgcagaa caaatcgcac ctgatggaag agttgaagaa
    16921 aatcgtccgc gtgatgaaga aactcgacgt tgaagcgccg catgaagtta tgctgactat
    16981 tgatgccagc accgggcaga acgcggtaag ccaggccaaa ctgttccatg aagccgttgg
    17041 cttaaccggc atcacgctaa cgaaactgga cggcacggcg aaaggcggag taattttctc
    17101 ggtggctgac cagtttggta tccctatccg ctacattggt gtcggcgaac gtattgagga
    17161 tttgcgtccg tttaaggcgg acgactttat agaggcactt tttgcccgag aggattaaca
    17221 atgattcgct ttgaacatgt cagcaaggct tatctcggtg ggagacaggc gctgcagggc
    17281 gttacgttcc atatgcagcc gggtgagatg gcgtttctga ccggtcattc cggcgcaggg
    17341 aaaagtaccc tcctgaagct gatctgtggg attgagcggc ccagcgccgg gaaaatctgg
    17401 tttagcggcc atgacatcac gcgtctgaaa aaccgtgaag ttccgtttct gcgccgccag
    17461 attggtatga ttttccagga tcaccatcta ctgatggacc gtactgtcta cgataacgtg
    17521 gcgatcccgc tgattatcgc cggtgccagc ggtgacgata ttcgtcgccg ggtgtcggcg
    17581 gcactggata aagtcgggct actggacaaa gcgaagaact ttcctattca gctttccggc
    17641 ggtgaacaac agcgtgttgg cattgcccgc gcggtggtga ataagcccgc ggtactgctg
    17701 gcggacgaac cgactggtaa cctggacgac gcgttgtcgg aaggcatttt acgtctgttt
    17761 gaagagttta accgcgttgg ggtaaccgta ttgatggcaa cgcacgacat caacctgatt
    17821 tcgcggcgtt cctatcgcat gctcaccctg agcgatggtc acttgcatgg aggcgtgggc
    17881 catgaataag cgcgatgcaa tcaatcatat tcggcagttt ggcgggcgtc tggatcgctt
    17941 ccgtaaatcg gtcggcggct caggcgacgg cggtcgtaac gcaccgaaac gcgcgaaatc
    18001 ctcgccaaaa ccggtaaatc gcaaaaccaa cgttttcaac gaacaggtgc gctatgcctt
    18061 ccacggcgca ttgcaggatc tgaaaagcaa accgttcgcc acgtttttaa cggtgatggt
    18121 tatcgccatc tccctgacgc tgcccagcgt ctgttatatg gtgtacaaaa acgttaacca
    18181 ggcggcgacg caatattatc cgtcaccgca aatcactgtt tatctgcaaa aaacgctgga
    18241 cgatgacgcc gctgcgggtg tggtggcaca gttacaggcc gagcaaggcg tagagaaagt
    18301 gaattatctt tctcgtgaag acgcactggg cgagttccgt aactggtctg gttttggtgg
    18361 tgcgctggat atgctggaag aaaacccgct tccggcagtg gcggtggtga tcccgaaact
    18421 cgattttcag gggacggaat cactgaatac gctgcgtgat cgtatcaccc agatcaacgg
    18481 cattgacgaa gtgcggatgg atgacagctg gtttgcccgt ctggcggcgt taaccgggct
    18541 ggtcgggcgc gtttccgcga tgatcggcgt gttgatggtg gcagcagtgt tcctcgtcat
    18601 cggcaacagt gtgcgtctga gcatctttgc tcgccgtgac tccattaacg tgcagaaact
    18661 gattggtgcg acggatggtt tcatcctgcg cccgttcctg tacggaggcg cattgctggg
    18721 attttctggc gcattgctgt cattaatttt gtcagaaatt ctggtactgc gtttgtcatc
    18781 ggcggttgcg gaagtggcac aggttttcgg aacaaagttt gatatcaatg gcttatcgtt
    18841 cgatgaatgc ctgctgttgc tgctggtatg ctcgatgatt ggctgggtgg cagcgtggct
    18901 tgccacggta caacatttac gccactttac gccggaataa taaaagcgtg ttatactctt
    18961 tccctgcaat gggttccgta gcagggaaag agaccccgtt gtctcttccc ggtatttcat
    19021 ctctatgtca cattttgtgc gtaatttatt cacaagcttg cattgaactt gtggataaaa
    19081 tcacggtctg ataaaacagt gaatgataac ctcgttgctc ttaagctctg gcacagttgt
    19141 tgctaccact gaagcgccag aagatatcga ttgagaggat ttgaatgact gacaaaatgc
    19201 aaagtttagc tttagcccca gttggcaacc tggattccta catccgggca gctaacgcgt
    19261 ggccgatgtt gtcggctgac gaggagcggg cgctggctga aaagctgcat taccatggcg
    19321 atctggaagc agctaaaacg ctgatcctgt ctcacctgcg gtttgttgtt catattgctc
    19381 gtaattatgc gggctatggc ctgccacagg cggatttgat tcaggaaggt aacatcggcc
    19441 tgatgaaagc agtgcgccgt tttaacccgg aagtgggtgt gcgcctggtc tccttcgccg
    19501 tccactggat caaagcagag atccacgaat acgttctgcg taactggcgt atcgtcaaag
    19561 ttgcgaccac caaagcgcag cgcaaactgt tcttcaacct gcgtaaaacc aagcagcgtc
    19621 tgggctggtt taatcaggat gaagttgaaa tggtggcccg tgaactgggc gtaaccagca
    19681 aagacgtacg tgagatggag tcgcgtatgg cggcacagga catgaccttt gacctgtctt
    19741 ccgatgacga ttccgacagc cagccgatgg ctccggtgct ctatctgcag gataaatcat
    19801 ctaactttgc cgacggcatt gaagatgata attgggaaga gcaagcggca aaccgtctga
    19861 ccgacgcgat gcaaggtctg gacgagcgta gccaggatat catccgcgcg cgctggctgg
    19921 atgaagacaa caagtccacg ttgcaggaac tggctgaccg ttacggtgtt tccgctgagc
    19981 gtgtgcgcca gctggaaaag aatgcgatga aaaaacttcg cgctgccatt gaagcgtaat
    20041 ttccgctact cagcagagaa ccctggatga gaatccgggg tttttgtttt ttgggcctct
    20101 gcaataatca gtttccccct ccggcaaaac gccaatcccc acgcagattg ttaataaact
    20161 gtcaaaatag ctattccaat atcataaaaa tcgggtatgt tttagcagag tatgctgcta
    20221 aagcacgggt agtcatgcat aaaacgaaat aaagtgctga acaacaacat cacaacacac
    20281 gtaataacca gaagaatggg gattctcagg atgaacataa agggtaaagc gttactggca
    20341 ggatgtatcg cgctggcatt cagcaatatg gctctggcag aagatattaa agtcgccgtc
    20401 gtaggcgcaa tgtccggtcc ggtggcgcag tacggtgacc aggagtttac tggcgcagag
    20461 caggcggttg cggatatcaa tgctaaaggt ggtatcaaag gcaacaaact gcaaatcgta
    20521 aaatatgacg atgcctgtga cccgaaacag gcggttgcgg tggcgaacaa agtcgttaac
    20581 gacggcatta aatacgtgat tggtcacctc tgctcttcat caacgcagcc agcgtctgac
    20641 atctacgaag acgaaggcat tttaatgatc accccagcgg caaccgcacc agagctgacc
    20701 gcccgtggct atcagctgat cctgcgcacc accggcctgg actccgacca ggggccgacg
    20761 gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta ttgctatcgt tcacgacaaa
    20821 cagcaatacg gcgaaggtct ggcgcgagcg gtgcaggacg gcctgaagaa aggcaatgct
    20881 aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag atttctcaac gctggtggcg
    20941 cgtctgaaga aagagaatat cgacttcgtt tactacggcg gttatcaccc ggaaatgggg
    21001 caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc agtttatggg gccggaaggc
    21061 gtggctaacg tctcgctgtc taacattgcg ggtgaatcag cggaaggact gctggtgacc
    21121 aagccgaaga actacgatca ggttccggcg aacaaaccca ttgttgacgc gatcaaagcg
    21181 aagaaacagg acccaagcgg cgcgttcgtc tggaccacct acgccgcgct gcaatctttg
    21241 caggtgggcc tgaatcagtc tgacgatccg gctgaaatag ccaaatacct gaaagcgaac
    21301 tccgtggata cggtgatggg gccgctgacc tgggatgaga aaggtgatct gaaaggcttt
    21361 gagttcggcg tatttgactg gcacgccaac ggcacggcga ctgatgcgaa gtaatcatta
    21421 atcggcaact ttgggttgcc gccaaattgc taatatcgag tacgttgctt catgccggat
    21481 gcggcgtaaa cgccttatcc ggcctacaag attcaaagaa atcagtaaat tgcagcacac
    21541 attgtaggcc tgataagcgt agcgcatcag gcaatatcac ttgtgaaatc aaacttgacg
    21601 attaacgctt ctcccagccg ccctgttgtg ccgtaaatcc cagtgcctgc ataaacgctg
    21661 tcatcacgcc gcgatcttcc acgcctgcat ccgccatcca ccagcatgaa acgccaggat
    21721 tgttacgcaa aacctcttcc agcagatatt gccccacacc gcgacggcgg gtgacttccc
    21781 tcacacgcag ggaatccagt gccccctcgg tgccgcttaa ggttacccgc acggccgcga
    21841 gaaggcgctc gttaaaacgc gcggcgtaga tacggtggtt atcgtcaacc tgtaaagagg
    21901 aaggggaata ctccggccag atcttttgca ggtcaatccg gtcctggtcg ctaaagtttt
    21961 ctaatcgaat gatggtcagc ttcatgggga acccgtgtaa atcacaaaag tggaaccagt
    22021 gtagcgaaat aatttaatcg gaggctttct c
//
LOCUS       DAZUCC010000066        22011 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZUCC010000066 DAZUCC010000000
VERSION     DAZUCC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.066"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            47..1723
                     /gene="ybaL"
                     /locus_tag="ACHY09_002186"
     CDS             47..1723
                     /gene="ybaL"
                     /locus_tag="ACHY09_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFS3885156.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(1807..3111)
                     /gene="gsk"
                     /locus_tag="ACHY09_002187"
     CDS             complement(1807..3111)
                     /gene="gsk"
                     /locus_tag="ACHY09_002187"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFS3885157.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAARVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            3263..4222
                     /gene="aes"
                     /locus_tag="ACHY09_002188"
     CDS             3263..4222
                     /gene="aes"
                     /locus_tag="ACHY09_002188"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFS3885158.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FTGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(4219..5181)
                     /gene="hemH"
                     /locus_tag="ACHY09_002189"
     CDS             complement(4219..5181)
                     /gene="hemH"
                     /locus_tag="ACHY09_002189"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFS3885159.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(5313..5957)
                     /gene="adk"
                     /locus_tag="ACHY09_002190"
     CDS             complement(5313..5957)
                     /gene="adk"
                     /locus_tag="ACHY09_002190"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS3885160.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            complement(6138..8012)
                     /gene="htpG"
                     /locus_tag="ACHY09_002191"
     CDS             complement(6138..8012)
                     /gene="htpG"
                     /locus_tag="ACHY09_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFS3885161.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(8122..8727)
                     /gene="recR"
                     /locus_tag="ACHY09_002192"
     CDS             complement(8122..8727)
                     /gene="recR"
                     /locus_tag="ACHY09_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS3885162.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(8727..9056)
                     /gene="ybaB"
                     /locus_tag="ACHY09_002193"
     CDS             complement(8727..9056)
                     /gene="ybaB"
                     /locus_tag="ACHY09_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS3885163.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(9109..11040)
                     /gene="dnaX"
                     /locus_tag="ACHY09_002194"
     CDS             complement(9109..11040)
                     /gene="dnaX"
                     /locus_tag="ACHY09_002194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS3885164.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNSVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(9712..9776)
                     /gene="dnaX"
                     /locus_tag="ACHY09_002194"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(11169..11720)
                     /gene="apt"
                     /locus_tag="ACHY09_002195"
     CDS             complement(11169..11720)
                     /gene="apt"
                     /locus_tag="ACHY09_002195"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS3885165.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(11873..12250)
                     /gene="ybaN"
                     /locus_tag="ACHY09_002196"
     CDS             complement(11873..12250)
                     /gene="ybaN"
                     /locus_tag="ACHY09_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFS3885166.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12320..12847
                     /gene="priC"
                     /locus_tag="ACHY09_002197"
     CDS             12320..12847
                     /gene="priC"
                     /locus_tag="ACHY09_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFS3885167.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            12861..13022
                     /gene="rsmS"
                     /locus_tag="ACHY09_002198"
     CDS             12861..13022
                     /gene="rsmS"
                     /locus_tag="ACHY09_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFS3885168.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(13075..13173)
                     /locus_tag="ACHY09_002199"
                     /pseudo
     CDS             complement(13075..13173)
                     /locus_tag="ACHY09_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13234..16596)
                     /gene="mscK"
                     /locus_tag="ACHY09_002200"
     CDS             complement(13234..16596)
                     /gene="mscK"
                     /locus_tag="ACHY09_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFS3885169.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLRDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(16724..17371)
                     /gene="acrR"
                     /locus_tag="ACHY09_002201"
     CDS             complement(16724..17371)
                     /gene="acrR"
                     /locus_tag="ACHY09_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFS3885170.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            17513..18706
                     /gene="acrA"
                     /locus_tag="ACHY09_002202"
     CDS             17513..18706
                     /gene="acrA"
                     /locus_tag="ACHY09_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFS3885171.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            18729..21878
                     /gene="acrB"
                     /locus_tag="ACHY09_002203"
     CDS             18729..21878
                     /gene="acrB"
                     /locus_tag="ACHY09_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFS3885172.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         5048 a         5781 c         5842 g         5340 t
ORIGIN      
        1 tttcccggcc aaaaatggac aataaccacc aggaaaagga gacggaatgc atcacgccac
       61 cccgcttatc accaccattg ttggcggcct tgtgctcgcc tttatcctcg gcatgctggc
      121 caataaacta cgtatttctc ctctggtggg atatctgtta gcgggtgtgc tggcaggacc
      181 attcactccg ggctttgttg ccgataccaa gcttgcgccg gaactggctg aactgggcgt
      241 cattctgttg atgtttggcg tgggtctgca cttttcgctg aaggatttga tggcggtaaa
      301 ggccatcgcc attcccggtg cgatcgccca gatagccgtg gcgacgctgc tgggtatggc
      361 gctctccgcc gtgctgggct ggtcgttaat gaccggtatc gtgttcggct tatgcctttc
      421 caccgccagt accgtggtgt tactgcgcgc acttgaagaa cggcaattaa ttgacagtca
      481 gcgtgggcaa atcgccatcg gttggttgat tgtggaagac ctggtcatgg ttctgacgct
      541 ggtgttgctg cccgcagtgg caggaatgat ggaacaaggc gatgtgggct ttgccactct
      601 tgctgtcgat atggggatca ccatcggcaa ggtgatcgca tttatcgcca ttatgatgct
      661 ggtaggtcgc cgtctggtgc cgtggattat ggcacgtagc gcagcaaccg gttcccgtga
      721 gctgtttacc ctgtcggtgc tggcgctggc gttagggatt gcctttggtg cggtggagct
      781 gtttgatgtc tcctttgcac tcggtgcgtt ctttgccggg atggtactga acgagtctga
      841 actgagtcac cgtgccgccc acgatacgct gccattgcgc gacgcgtttg cggtgctgtt
      901 ttttgtctcc gtcgggatgt tgtttgatcc gttaattctg attcagcaac cgctggcagt
      961 gctggcgacg ctggcgatta ttctgtttgg taagtcgtta gccgcgtttt tcctggtgcg
     1021 actgtttggt cactcccaac gtacggcatt aactatcgcc gccagcctgg cgcaaattgg
     1081 tgagttcgcg tttatcctgg cgggactggg catggcgctg gatctattgc cgcaagccgg
     1141 gcagaacctg gtactggcag gggcgatttt gtcgattatg ctcaacccgg tcctgtttgc
     1201 gctgctggag aaatatctgg cgaagaccga aacgctggaa gagcagacgc tggaagaggc
     1261 tatcgaagaa gagaagcaga tcccagtgga tatttgcaac catgcgctac tggtaggtta
     1321 cggtcgtgta ggcagcctgc tgggggagaa attgctcgcc tctgatattc cgctggtggt
     1381 gattgagacg tcacgaaccc gtgttgatga actgcgagag cgcggggtcc gcgcagtatt
     1441 gggcaatgcg gcgaacgaag aaattatgca actggcgcat ctggaatgtg caaaatggct
     1501 gatcctgacg attcccaacg gttatgaagc gggcgagatt gtggcatctg cccgcgcgaa
     1561 aaatccggat attgagatta ttgcccgcgc ccattatgac gatgaagtgg cgtatatcac
     1621 cgaacgtggt gcgaatcagg tagtgatggg tgagcgtgaa attgctcgta ctatgctgga
     1681 actgctggaa acgccaccgg cgggtgaggt ggtgacgggg taaaacattg attccattgc
     1741 cggatgcgat gctgacgcat cttatccggc ctacggatgc catcgtcgtc cggtctggtc
     1801 gataacttaa cgatcccagt aagactcttc caggctgtct tcacgctccg gcaagccgcg
     1861 cgttaaacga ggtgaatgct ggttcagtac ctgatagctc acacggttag catatttaca
     1921 cacctgcgct aacgatgaat aagttaacca ggtgaattta tgtttgctgg agtttggtac
     1981 gttgctacgg tggtagctgt tggcggtaat gtcatgcagc aacgctgcca gtgcgccatc
     2041 ccctgctcca ttagtgttca tgattttttc cggcccgccc atgtacggcg caatgtgcga
     2101 atagacacgc agcggattct ggcaatcctt gtggcgcatg gcgcggctaa actcatactg
     2161 gttaaattcc gctatagcac ccggcagcag cggatgctgg gttttacgtt tcgcttcgtc
     2221 ttcggtaaag cccgccatat acaagccgat tggccctgcg gtacacagca ccagatcaac
     2281 ccagtccagc gccttgtcag atgccaacaa cggatcgctt tctccggtca acgcttcggc
     2341 ttcatcttcg ttcatcgcaa ggatagagac gtggtctttg aggaattgct gccaccactg
     2401 cggattctcg gcaatgacaa acttggtgcc cagcgtcagc accaccggta cgttatattt
     2461 cttcgcgtac tcaatagctt tcatggttgc ttccggcatg ggttcacccg gcttgcaacg
     2521 caccagataa gaggtgagaa ccagagccga ggctccggca atcacatctt ccggaatact
     2581 ttcagcccgc agctggttca tgtggccggg gctgatagca aaggtacgtt ccccggactc
     2641 gccaatcagc gtaaagcaac gaccaatcgg gccatccacg ccttgtagat agttaagatc
     2701 ggtacggctg gaagtgttac acaggtaacg ataggcatag ctgccaattt caatattgct
     2761 gcacatgacg cccagcagca ccgaacggtc gtccgcgagc accgagtagt tgtgcatggt
     2821 gttaccaatg gtgccacccg caaactgatg ggtaatcagg tttttctgtt ttagttcctg
     2881 ataaagcgct tcggctacgt catcctcaat caccagtgaa tgcccggcgc ttaatccata
     2941 acgctcaata aattcatcat ccactttcgc ttcaatatcg accagcgttt gatcgatacc
     3001 cactaccctg gcagcgctgg tttcgttttc tggctggaat tgctgaagca gcggatcgcg
     3061 tgcgtttacg ggaaaataat gtttggattt acgtttgccg ggaaatttca tggttgtttt
     3121 tacgggtggt taactgagcg gcgaatgtta acacaatctg acgggagcgc gcgagacgca
     3181 gccagtttca atatcagcag gcgggatcgc attttcatgg cagtggttcc ttacaatgac
     3241 gtaatttgaa aggagttttt gcatgaagcc ggaaaacaaa ttacccgttc tggaccttat
     3301 ttctgctgaa atgaagaccg ttgtgaatac tcttcagccg gatttaccgc cctggcccgc
     3361 aacgggaacg attgctgagc aacgacagta ttacacgctt gagcgccgat tctggaatgc
     3421 gggcgctcca gaaatggcaa ccagagctta catggttcca acaaaatatg ggcaggtgga
     3481 aacacgtctc ttttgtccgc agccagatag cccggcgacg ctattttatt tgcatggcgg
     3541 cggttttatt ctcggcaatc ttgataccca cgatcgcatc atgcgcctgc tggcaagcta
     3601 cagccaatgt acggtgattg gcattgatta caccctttca cctgaagcgc gttttccgca
     3661 agcaatagag gaaattgtgg ctgcctgttg ttatttccac cagcaggcgg aagattatca
     3721 aatcaatatg tcccgcattg gctttaccgg tgattccgca ggtgccatgt tggcgctcgc
     3781 cagtgcgttg tggttgcgtg ataaacagat cgattgcggt aaagttgcgg gcgttttgct
     3841 gtggtatggg ctttacggat tacgggattc cgtgactcgt cgtctgttgg gcggtgtctg
     3901 ggatggctta acgcaacagg atttacagat gtatgaagag gcgtatttaa gcaacgacgc
     3961 ggaccgcgag tcgccgtatt actgcctgtt taataatgat ctcactcgcg aagttccgcc
     4021 ctgttttatt gccggggcgg agttcgatcc gctgctggat gacagccgtc tgctttacca
     4081 gacgttagcg gcgcatcagc agccctgtga gttcaaactc tacccaggca cgctgcacgc
     4141 ctttttgcat tattcacgga tgatgaaaac cgccgacgag gctcttcgcg acggcgctca
     4201 gttctttacc gctcagcttt agcgatacgc ggcaacaaga ttagccatca tttcaatatg
     4261 ttccggcgtg gcattaagtg ccggaatata ttcgtatttt ttcccgcccg caccgaggaa
     4321 gacctcacgg ttttgctcgg caatctcttc cagcgtctcc agacaatccg cagcaaagcc
     4381 cgggcacatc acctgaatat gacctacacc tttttctccg agcattttca gcgtttcgtc
     4441 ggtataaggc atcagccagg gttcccgccc aaagcgcgac tgaaaggtca tcatcacttt
     4501 ttccggtgcc atccccagtg cggaagccag ttcgcgagtc gttgtgcgac aacgttgcgg
     4561 gtaatcatcg ccttcatctg cataacgctg gggaatgcca tgataagaga gcaacagcag
     4621 atccggttcg ccatgtttgg caaaagaagc acgtacgctg ttcgccagtg cattaatata
     4681 atcgtggtta tcggcgtaat cacgaataaa cgatatcccc ggaatgctac gtttgcgcgc
     4741 cagaatgcgt gccagttcat cccataccgc accgaccgtt gaacaggaga attgcggata
     4801 aagcggcagc accacaatat gatctacatg ctctgccagt agttcatcta cggcgctttc
     4861 cagtgatggc gagccgtagc tcattcccag cgctacgggc gtctccggta aacgttgtgc
     4921 cagcgcctgc tgttgctggc ggctgtaaac catcagcggc gagccacctt ccatccagac
     4981 agaggcatac agcttcgcca cacgcggcga gcgcagtggc aaaatcacgc cgcgcagcaa
     5041 tggccaccat aacaaccgtg aggtatcaac cacacgtctg tcgcttaaaa attgtttcag
     5101 atagcgtttt accgcttcag gtgtgggggc atcgggcgta cccaggtttg ccagcaggat
     5161 accggttttt gtctgacgca ttaccgcctc ttatcgattc aacttgttga taattgtagc
     5221 ggaaaagtga agaagaagaa ctaattgctg tgagaggtat tgcctgagga ggatgagcag
     5281 acctgcccga aagcagatct gctgcggctt gattagccga gaattttttc cagagctgcg
     5341 cgaacttcag ccaccggctt ggtgccgtca actttcgcgt atttggtgtt acccgcttcc
     5401 gcttctttag agtagtagcc gatcagcggt gcagtcatct gatggtattc aaccagacgt
     5461 ttacgtacgg tctcttcctg atcatcttta cgggtagtca gctcttcacc ggtaacgtcg
     5521 tctttgcctt ctactttcgg cggattgaat ttaacgtgat aaacacgacc agacggcgcg
     5581 tgaacgcggc ggcctacgat acggtcaaca atcagttcgt ccggtacgtc gaattccaga
     5641 acgtaatcaa cattgatgcc cgcttctttc atcgcgtctg cctgcggaat ggtacgcggg
     5701 aagccgtcca acaggaaacc gttgcggcag tcttcctgag caatgcgctc tttaaccagc
     5761 gcgatcacca gttcgtcggt gaccagtttg ccagcatcca taatgtcttt tgcttgttta
     5821 cccagctcgg agccagattt gaccgcagca cgcagcatat cgccagtgga gatttgcgga
     5881 ataccatatt tctccatgat gaactgagcc tgagtccctt tccccgcgcc cggagcgcca
     5941 agcagaatga tacgcattgc gaaaatcccc ttaaaatgtg tcgaattttt tgaaaaagcg
     6001 ataaacgata ccaccaacag gcgattgcct caagaaaggc acctggggct gaaacggtta
     6061 atggcgagat aatttgcgga aagtgcagat gatgaaaaga aaaatgccgg atgacacaaa
     6121 ggtcatccgg catgacatca ggaaaccagc agctggttca tacgacgaat aaacaggttc
     6181 ggatcttcca gcgtgccgcg ttctgccagc agcgcctgat ccagcagcag ttctacccac
     6241 tcgctgaact tggcttcatc ttcagtatct gccgcacgtt tcaccagtac gtgatccggg
     6301 ttcagttcga agatgtattt cacttctggc actttctggc ccgccgcagc gaacagtttc
     6361 gccatctgag tgctcatttc gtccgcgtcg gtcgaaacga tcgctggcgt atcggtcaga
     6421 cggtgagtca gacggacatc tttcacgcgc tcgccgagca gggctttcac acggtcgata
     6481 aacggagtca gtgctttctc cgcttctttc gcgctctcat caacttcgtc agccagtttt
     6541 tccagcgact cgtcaacttt agacacagac tggaacggtt taccgtcgaa ctcagtcaga
     6601 tagttcatca tccactcatc gatgcggtcg gaaagcagca gaacttcgat gcctttctta
     6661 cgcagcagtt ccaggtgcgg gctgctcttc gctgccgcgt agctgtctgc agtgatgtag
     6721 tagattttct cctgcccttc tttcatgcgg gaaacgtagt cttccagaga cacggtctgc
     6781 gcagaagaat cggtatgggt agacgcaaac cgcagcagtt tggcgatcgc ttcctggtta
     6841 gcgaaatctt ccgccggacc ttctttcagt accaggccaa actgttgcca aaaggtctgg
     6901 tatttttccg cgtcgtcttt cgccagtttt tccagcattt gcagcacacg cttggtcagc
     6961 gcattgcgca ggttacgcgt taccgtgctg tcctggagga tttcacggga aacgttcagc
     7021 ggcagatcgc tggagtcaat cagaccacgc acgaagcgca gatagttcgg catgaactgt
     7081 tctgcgtcgt ccatgatgaa cacacgctga acatacagtt tcagaccgtg tttatgatcg
     7141 cggttccaca tatcccacgg tgcctgagac ggaatgtaca gcaggctggt gtactcctgc
     7201 ttaccttcaa cgcggttgtg gctccaggtc agcggatcgt taaagtcgtg ggcgatgtgt
     7261 ttgtagaact ctttgtactc ttcatcggtg atttccgact tgttacgagt ccacagcgcc
     7321 tgcgctttgt tgattttctc ccaggagata acggtttcgc cgtctttctc ttcgcgtttt
     7381 tcgatctcta ccggcagcgc gatatggtcg gaatatttgc tgatgatgga acgcacacgc
     7441 cagtcatcga ggaactcgtc ttcgccttca cgcagatgca gggtgatttc agtaccacga
     7501 tcttctttgg tgatgtcggc aacagtgtat tcaccttcac cagccgattc ccagaagacg
     7561 ccattttctg gtttttcgcc tgctgcgcga gtacgcacgg tcactttgtc agccacgata
     7621 aacgcagagt agaaaccaac accaaactga ccgatcagct ggctgtcttt cgcctggtca
     7681 gaacccaggg attcgaggaa tgatttggtg ccggatttag cgatagtccc cagatggtca
     7741 atcacttcgt cgcgggtcat ccccacgccg ttatcggaga tggtcagcgt gcgcttgtct
     7801 ttatcgaaag agacacgaac gcgtagttca ccatcacctt cgtacaggtc cgggttagaa
     7861 agcgcgcgga aacgcagctt gtccgccgca tcggaggcgt tagagataag ctcacgcagg
     7921 aagatttctt tattggaata gagagaatgg atcatcaggt gcagaagctg tttcacttct
     7981 gactgaaaac cacgagtttc ttgtcctttc atgtaggtct acctcaataa tgccatttta
     8041 aggttaaaaa caggatgagg gagagatggg gacaagggag aataatttca agcgagagca
     8101 ggtgatcctg ctctcgtttg cttaaaaacg aatcttatga cgcccggcaa gggaatgtga
     8161 cagcgtggtg ccatcgacca tttccagttc gccgccaacc ggtacgccat gagcgattcg
     8221 gctggcttcc acgtcatatt gcgcacaaag ctcggcaatg tagttagcgg tagcttcacc
     8281 ttcaaccgtg gggttggtgg cgaggatcac ttcagtgatt ttttcctctg ccagacgctg
     8341 ttccagacga tcaagcccga tatcatcagg accgatgccg tccagcggtg acaggtgtcc
     8401 catcaacaca aaataacgac ctgaaaactg ccccgtctgc tcaatggcgt aaatgtccgc
     8461 cggactctcc accacgcaga tttgaccgtt ttcctgacga cgtggattcg aacagatgtt
     8521 acagacttcc tgttcggtga aggtgcggca atcggcgcag tggccgattt ccgacatcgc
     8581 ccgggtgagc gcctgcgcca gacgcatccc gccgctacga tcgcgctgaa gcagcgtgaa
     8641 cgccatacgc tgcgccgact tcgggccaac gcccggcaga cagcgcagtg cttccataag
     8701 ctgtgttaac agcgggctgg tttgcatcag aacggcatct taaagccagg cggcagctgc
     8761 attccggagg atacagaggc cattttttct ttctgcgttt cttcaatacg acgtgctgcg
     8821 tcgttgaatg ctgcagccac caggtcttcc agcatctctt tgtcgtcttc cagcaggctc
     8881 gggtcgatct ctacgcgacg gcagttgtgt gcaccgttga tggtcacttt taccagacct
     8941 gcgccagatt cgccggtgac ttccagctgc gcgatctctt cctgcatttt ctgcattttt
     9001 tcttgcatct gctgggcttg cttcatcagg ttacccagac cgcctttacc aaacataggt
     9061 ttctctctca atcacgttaa ggatgacgaa cgtaagctgt gcttacgatc aaatggggcg
     9121 gatactttct tcatccagct ccgcatcgaa gaatcgacgc agggtctgaa tattattatc
     9181 cgcaataatg gactcgcgcg cctgcgcaag tttttcttcg tatatcgcct gacgccattc
     9241 cagcggcgta cgcaccgcgg gattatcatc ttcaacgata gtcagttcaa ccgttgaacc
     9301 ttttaacgtg ctcaacgctt cagccagttt ttgctgtgcg ccgcggttgt tcaaatgccg
     9361 ctgagaggag cgcaaatgca gacatactga gttgtcgctc tcctctttcc aggcatttaa
     9421 cgccacctgt tcgaccagtt ttggtagcga aagttgactc acctgagccg cccacgggtc
     9481 gcgctcaatg gcttctgccg ccagcttcgc cgccagttcc ggcgtttttt catgttccag
     9541 cgcttttttc agcgccttcg gcgtggcgac cacttctttt tgctgcatca ctggagtggt
     9601 cgccttccag cgatacgctt cttttttggc tggcgctttt tccagcgccg atggcaccgg
     9661 gcgcgcctga acgcgatcgg tgaccgaagc cagtctttcc agcgcagcgt tattcaccgg
     9721 ccgcgcgcgg gtagcggctg ccggttcact cttttttgct ttggttgctc cctgcacgcg
     9781 ctgcaactgc tggcgcgccg ccagcacctg gctggtggtt tccgggagcg gtacagtcgg
     9841 tgcctgctgc ggcgctgatt gcggttgcgg cggcacctgt gttggcgtca ttactgccgt
     9901 tggcgcaacg ggcgcaaagg actggcgtgg cacttctggc tcaggcagag gcatacgcgg
     9961 atggaacgcc agcgcgcgca gcagcgtcat ctcaacaccc atacggcggt ctggcgcata
    10021 cggtaattct ttgcgaccaa tcaacagcgt ctgatagtaa agctgaatat ccgtcggcgg
    10081 tatggtgcgc gccagttcac gcatccgcag ctcaatggcg gccatgtcgt tgccaagagc
    10141 agcaggcgaa agttgtacca tcgcaatacg atgcaacaga ccgagcattt ccaccagcaa
    10201 cgcttcccac tcgataccac gggcagcagc ttcattaatt agcgccatta cgcgctcgcc
    10261 gttggcctcg accatcgctt caaccagcga taacgcctga tcgtcgtcaa gcgtacccag
    10321 catcgcactg accgcctggg ttgaaacctg gccgtcaccg ctggcaatcg cctggtcggt
    10381 cagacttaaa gcatctcgca ggctgccttc agcggcgcgt gccaacaatt gcagcgcccg
    10441 cggctcgtga gcgatatgtt cttcgttgag gatgtgctca agctgatggc gaatttgctc
    10501 gacatccagc gccttgagat gaaattgcag acagcgtgac aaaatcgtca ccggcaattt
    10561 ctgcggatcg gtcgtcgcca gcaaaaactt aacgtgctct ggcggctctt caagggtttt
    10621 taacagtgcg ttaaagctgt gacgcgacag catatgcact tcgtcgatga gataaacttt
    10681 gaaacgacca cgcgccggag cgtactgaac gttatccagc aggtcgcggg tatcttcgac
    10741 tttagtgcgc gaggcggcgt cgatttcaat cagatcgaca aagcgcccct gctcgatttc
    10801 acgacagtta tcgcacacgc cgcacggcgt cgcggtaatg ccggtttcgc agtttagccc
    10861 cttcgccagc agtcgggcga tagaggtttt tccgacgcca cgggtaccgg aaaaaagata
    10921 agcatgatga atacgcccta acgacaagcc gttcgccagt gcggtcagca catgttcctg
    10981 gccgacgacg tcagcaaagg tttgtgggcg ccattttcgg gctaagacct gataactcat
    11041 tggcaggctc tgaaacgctg gaaggtggat tcacgaaggg gtaatgctaa cacagccccg
    11101 tcagaacggc gaggctgaat gcacatcttg tgctgtccgt agcgtgggca gcacaagact
    11161 ggcgataatt aatggcccgg gaacgggaca aggctgtagc tggtaatgcc ctgtttttcg
    11221 agacgctgtt cgccgccgag atcgaacagg ttgataatga acgcagcgtc agccacttca
    11281 ccacccagac gacggatcag tttaacggtc gcttcgatag tgccgccggt tgccagcaga
    11341 tcgtccacca ccagaacttt gtcgcccggt ttgatggcat caacgtggat ttccagctga
    11401 tcggtgccgt attccaggtc gtaagtttca ctgatggttt cacgcggcag tttgcccggt
    11461 ttacggaccg gtacaaagcc aacgcccaga cccagagcta ccggagcgcc aaacaagaag
    11521 ccacgcgctt cggtgccgac aactttggta atgcccgcat ttttgtaacg ctcaaccagc
    11581 aagtcgatgc tgagagcgta agctttcggg tcttccagta agctggtgac atcacggaaa
    11641 agaatgccgg gttttgggta gtcctgaatg cttttgatgc tatttttgag atactcaagc
    11701 tgctgtgcag tcgcggtcat aagtgtatgc ctgcttgtta cggtggtact cacggcgcgt
    11761 tttaaacgta tcaaaagtaa cagttgttta acctttgacc gcctgcgcct gtgctcgaaa
    11821 acgccagaat gtactggctg tacgcaataa ttgcaactgc gattgttgtg cttcagtgct
    11881 tttcttgctt ttcatcaatc accggaattc gccacatata aaaaagcaaa caggcgagaa
    11941 ttaccagcaa catgatgcgc acccatggca tctggacgaa ccacagagaa atggcaaacg
    12001 tgagcaaaat aagcaaaatc gcccgcggtt tgacgccgcg cggcatcgca tgatgtttct
    12061 gccagaaacg tagatagctg ccaaaccatg agcggtacag caaccaggcg tgaaagcgcg
    12121 gggaagaacg ggcaaagcac caggccgcca gcaggataaa cggcgtcgtc ggtaataccg
    12181 gtaataccac gcccagcgta cccagcacta ccgccagcca gccaatgatg attaaaatga
    12241 ttcgttgcat attgagtgtt gtcagcttac agagttgcta ctttagcata acaattatca
    12301 ttttcattga ggtcttatcg tgaaaaccgc cctgctgctg gaaaaactgg aaggacagct
    12361 cgctacgctg cgtcagcgtt gtgccccggt agcgcaattt gccacgctaa gcgcccgttt
    12421 tgacaggcat ctttttcaga ctcgtgcgac aacactgcag gcttgtctcg atgaggcggg
    12481 cgataatctg gctgcgcttc gtcatgcggt tgagcagcaa cagctgccgc aagtcgcctg
    12541 gctggcagaa catctggcgg cgcaactgga ggctatagcg cgtgaagcca cagcctggtc
    12601 gctacgcgag tgggatagcg caccaccgaa aattgcccgc tggcagcgta aacgtattca
    12661 gcatcaggat tttgagcggc ggctacgtga gatggttgcc gaacgtagag cccgtctggc
    12721 acgagcgacc gatctcgtgg aacagcagac gctgcatcgt gaagtggaag cctatgaagc
    12781 gcgcctggca cgctgccgcc atgcgctgga aaaaatcgaa aacaggttag cgcgtttaac
    12841 ccgctagcaa tggagagagt atgtcactgg aaaatgcccc tgacgatgtc aaactggccg
    12901 tcgatttgat tgtgctactg gaagaaaatc agatcccagc ccgcaccgtg ctgcgcgcgc
    12961 tggatattgt aaaacgcgat tatgaaaaga aattaacgcg cgatgatgag gcggaaaagt
    13021 gagaaataaa tggggccgct aaatgtcccc taagattgtg ggcaaacgca aaactgcctg
    13081 atgcgctacg cttatcaggc ctacaccgct cctgcaatat attgaattct tgcggtttcg
    13141 taggccggat aaggcggtca cgccgcatcc ggcatgaaca acgcgcactt tgttagtaat
    13201 ctcggggcaa ctaaatgtcg ccccctttgg ttattaccct accgctggcg tcggatcgtc
    13261 gcctttgtag tcgcgtttga cttccgtcac ctcatcgccc ttctcgttgt gcagatgcac
    13321 ttcaagctgg ttaaaggcaa tgttgatgtc gttttcacgg cacaactgat cgatagtacg
    13381 gttcagctca tcgacagtac ggctacggtc acgcagttca cgcacataca gacgcagctc
    13441 atgatccaac gtgctggcac caaatgccgt aaagaagact tccggcattg gttcgtgcat
    13501 cacccttggg tgctcagtcg ccgccttcag caacacttta cgcacttttt ccagatcgga
    13561 gccataagct acgccgagac ggatcaccag acgcgtagtc gtgtcagtca acgaccagtt
    13621 gatcagacgc tcggtaacaa acgctttgtt cgggatgatc acttctttgc gatcgaaatc
    13681 ggtaatcgtt gtcgcacgaa tacggatctt acttaccgtc cccgagaagc taccaatggt
    13741 taccgtgtcg cctatgcgca ccggacgttc gaacagaatg atcaaaccgg aaacgaagtt
    13801 accgaagatc tcttgtaaac caaaaccaag acctacggat aatgctgccg ccagccactg
    13861 gagtttatcc cacgagacgc ccagcgatcc gaacaccgtc atcgcaccaa cggcaataat
    13921 gatgtagtta aggatggtag taatggcata cgacgcgccc tggcgcatgt tcaggcgcga
    13981 gagcaccaac acttccagca aaccaggcag gttgcgaatc aacgcccagg ccaccattga
    14041 ggcgataatc gcaaacaaca gactgcccat ggtgacgttt ttcaccaccg cagcaccagc
    14101 ttcagtgccg ttgtaatgcc agagcgtgat gctgtccaga tagctgaaca cggtgatcaa
    14161 atcggaccaa attgcccaga acatgacacc gaacagcgca aacatcagca acatggtaat
    14221 acgcagcgtc tgctggttaa cctgctccag tgcaatggtg ggttcttccg gcggttcagc
    14281 accttctgcg ccctctttca ccagattctg ccgacgcgcc agcgcacgac gccaggcgat
    14341 acgccgcgcc gctacgctta agccgcgcaa taccgtctgg tacagcaggt tccagatgat
    14401 caccaaataa acggtttcaa tccaacgccc ggagaggcgc agcgtggtgt agaaataacc
    14461 cgtggcggtc agcaccatca acgcaatcgg gataatcgac agtacggtaa tggtgaccag
    14521 tcgcatggtg tgcgactctt tatcacgcca gctttcgcgg cacatcggcc acaccaggaa
    14581 ggcaatcagc agcaagttga agaaaatcat cgcctgcccc agcacatcat ccatcagatg
    14641 cagcggggaa agttctgcca ccacagacca gaaatggata ggcagcaacg cgagactgat
    14701 gcggacaatt tgccgacgcc agtggctggt ctgctgttcc ggcataccaa agtgacgcac
    14761 ggcaacgccg tttttctcca acactttcca gcacaggcca aacaccagcc agaaaatcgc
    14821 cagtttttta ctgaacgacc atagcagttc gctgatgttg agctgcatgg tcaacagaat
    14881 caggccaacc gcgagaataa tcaggcacac cggcagcgca cggatcaggt cgataaggat
    14941 cgcttttggt gtgttgagct ggctgtcgtt acgcagggaa cccacagcgg aagccagttt
    15001 ttgttgatac gctttcagcc agcccagacg ccagtggatc agcccggcaa tcaacagcag
    15061 cggcaaacca gcgaggaaag cgataaaaac ggcaggccag gctttttccc agttcaccgt
    15121 gattttcatc gacttaaatt catctctcag gctttgcggg aacgctttga tccagtccca
    15181 gtccattgga cggttactat tcacccaaaa gatttgctga gtcaggatgg atttcaggtt
    15241 tttcgacaca ctcattaact gctgctggtt gatttgcagg ttaatggcca tcatcagctg
    15301 gttacccaac tgtttgttaa gctgatccag caattcgcga cgcatatcaa ccacttgcaa
    15361 taacgcatcg tgaacttcgc tgttgacttc gttggtgtga ccttcttcca gtttgttgac
    15421 gaacgcatcg ctctggaaga gtgcatcacg ctgctggtta acttcaaact gttcgagacg
    15481 caaatccgcg atgcggttgg tcatgttttc cagttcatcc gccgagggca gcgtttgttg
    15541 ttgctggtaa aggatacgag acaacagcag gctgcccttc aggacggcaa tctgctcttt
    15601 aatattgcgt tccgattgca gcgcccgctc cagccagttt ttgactttaa tgttttgctg
    15661 catcaactga ttaccgtttt cagtcgcggt aatcagacgc tgacttaact gctggttaat
    15721 ttccagttcc tgcttcacca gcggattagc ctgaatacgc gcggcttcat ccggggagac
    15781 ggcttcctgc gccgtttttt cggttaaagt caagcgcttg ctgtttactg cttcttgcaa
    15841 cagctgcaac tggtgctcca gacgggcgct gttcgccgtc acgtaatccc gttgcttttg
    15901 caaggtatcc tgtaagactg tgttcccttc caggctttta cgctgctggt caatctcggc
    15961 attcagcaaa gcctgctgag cctgcattaa cactttctgg ctgggacgta aggcagtctc
    16021 gccgacatca gtcccatcca gacgactgcg aatttgttgc agctgctgcg aagcgttata
    16081 catcgcattt tgcacgcgtt cgggctgcgt ctgtaacgaa accagctggc tgttataaga
    16141 cgccagatcg ttttgtgcgt tttgcaaatc gtccagcgcc tgggcaacgc gagtttccag
    16201 ctggcgcaac gaaagcgtac tcagaatttt gcgagtttct tcgtcattat cgacatcgct
    16261 aagtgcagtt aacgccgcgg tcgcctggcg cattttttcc ggcgcttcag caaccttttg
    16321 ccgtagctga actgtctctt cttttacgcg atcgatttta tcgagggtag ctaatgtatc
    16381 tgtcagatcc tgctgcacca gtttgtcctg agcagaaaga tctttttgtt tattcagtga
    16441 gtcaagttgt gcctgcaggt ccgcttttgt cggcagatca ccattcgatg acgcccgcgc
    16501 aaacgccgtg ttctggcata acaagacaaa aacaaaagca ataaatgctg aaaaaacaaa
    16561 atgccgtgat cgtttgtaat actggaacat agtcatgatg aatgaaggtt tctgaacctg
    16621 aagaacgacc tgaaaaagtc aaaccgcaag aatatcacga cgcagtgaac cagaatagca
    16681 acgacgaaaa tgtccaggaa aaatcctgga gtcagattca aggttattcg ttagtggcag
    16741 gattacgaag cgtggggcac aggagataca tctccagtaa gatggcgacg taatcgcggg
    16801 cttctttttt aagatcaaaa gattgcgggg caaagagcca gttttccatc aggccggaaa
    16861 tatagccgcg cataataatt gctgcccgac gcgtcattaa atccgcaggc aacattttcg
    16921 cttcaataca atgttttaac gtttgttcta tacggtcata actttccaga cagagattac
    16981 gttgtgcctg ttgcacaaca gccatttctc cgacaaattc gcatttgtgg aatataatct
    17041 ccatcaataa tcgacgccgt tcttctgtca ccgtggattc aagaacatga attaatatct
    17101 ctcttaatac tgagagtgga tcgccaggga attttgcctg atactcaagc tcgagttcac
    17161 caatattgga ttctgacagt tcccagatct cactgaacaa atccgacttg tctttaaaat
    17221 gccagtagat tgcaccgcgc gtaacgccag ctgcttttgc aatctcgccc agcgaggtgg
    17281 atgatacccc ctgctgtgag aaaagacgta gagccacatc gaggatgtgt tggcgcgttt
    17341 cttgcgcttc ttgtttggtt tttcgtgcca tatgttcgtg aatttacagg cgttagattt
    17401 acatacattt gtgaatgtat gtaccatagc acgacgataa tataaacgca gcaatgggtt
    17461 tattaacttt tgaccattga ccaatttgaa atcggacact cgaggtttac atatgaacaa
    17521 aaacagaggg tttacgcctc tggcggtcgt tctgatgctc tcaggcagct tagccctaac
    17581 aggatgtgac gacaaacagg cccaacaagg tggccagcag atgcccgccg ttggcgtagt
    17641 aacagtcaaa actgaacctc tgcagatcac aaccgagctt ccgggtcgca ccagtgccta
    17701 ccggatcgca gaagttcgtc ctcaagttag cgggattatc ctgaagcgta atttcaaaga
    17761 aggtagcgac atcgaagcag gtgtctctct ctatcagatt gatcctgcga cctatcaggc
    17821 ggcatacgac agtgcgaaag gtgatttggc gaaagcccag gctgcagcca atatcgcgca
    17881 attgacggtg aatcgttatc agaaactgct cggtactcag tacatcagta aacaagagta
    17941 cgatcaggct ctggctgatg cgcaacaggc gaatgctgcg gtcactgcgg cgaaagctgc
    18001 cgttgaaact gcgcggatca atctggctta caccaaagtc acctcgccga ttagcggtcg
    18061 cattggtaag tcgaacgtga cggaaggcgc attggtacag aacggtcagg cgactgcgct
    18121 ggcaaccgtg cagcaacttg atccgatcta cgttgatgtg acccagtcca gcaacgactt
    18181 cctgcgcctg aaacaggaac tggcgaatgg cacgttgaaa caagaaaacg gcaaagccaa
    18241 agtgtcgctg atcaccagtg acggcattaa gttcccgcag gacggtacgc tggaattctc
    18301 tgacgttacc gttgatcaga ccaccgggtc tatcacccta cgcgctatct tcccgaaccc
    18361 ggatcacact ctgctgccgg gtatgttcgt gcgggcacgt ctggaagaag ggcttaatcc
    18421 aaacgctatt ttagtcccgc aacagggcgt aacccgtacg ccgcgtggcg atgccaccgt
    18481 actggtggtt ggcgcggatg acaaagtgga aacccgtccg atcgttgcaa gccaggctat
    18541 cggcgataag tggctggtga cagaaggtct gaaagcaggc gatcgcgtag taataagtgg
    18601 gctgcagaaa gtgcgtcctg gtgtccaggt aaaagcacaa gaagttaccg ctgataataa
    18661 ccagcaagcc gcaagcggtg ctcagcctga acagtccaag tcttaactta aacaggagcc
    18721 gttaagacat gcctaatttc tttatcgatc gcccgatttt tgcgtgggtg atcgccatta
    18781 tcatcatgtt ggcagggggg ctggcgatcc tcaaactgcc ggtggcgcaa tatcctacga
    18841 ttgcaccgcc ggcagtaacg atctccgcct cctaccctgg cgctgatgcg aaaacagtgc
    18901 aggacacggt gacacaggtt atcgaacaga atatgaacgg tatcgataac ctgatgtaca
    18961 tgtcctctaa cagtgactcc acgggtaccg tgcagatcac cctgaccttt gagtctggta
    19021 ctgatgcgga tatcgcgcag gttcaggtgc agaacaaact gcagctggcg atgccgttgc
    19081 tgccgcaaga agtacagcag caaggggtga gcgttgagaa atcatccagc agcttcctga
    19141 tggttgtcgg cgttatcaac accgatggca ccatgacgca ggaggatatc tctgactacg
    19201 tggcagcgaa tatgaaagat gccatcagcc gtacgtcggg cgtgggtgac gttcagttgt
    19261 tcggttcaca gtatgcgatg cgtatctgga tgaacccgaa tgaactgaac aaattccagc
    19321 taacgccggt tgatgtcatt accgccatca aagcgcagaa cgcccaggtt gcggcgggtc
    19381 agctcggtgg tacgccgccg gtgaaaggcc aacagcttaa cgcctctatt attgctcaga
    19441 cgcgtctgac ctctactgaa gagttcggca aaatcctgct gaaagtgaat caggatggtt
    19501 cccgcgtgct gctgcgtgat gtggcgaaaa ttgagctggg tggtgagaac tacgacatca
    19561 tcgcagagtt taacggccaa ccggcttccg gtctggggat caagctggcg accggtgcaa
    19621 acgcgctgga taccgctgcg gcaatccgtg ctgaactggc gaagatggaa ccgttcttcc
    19681 cgtcgggtct gaaaattgtt tacccgtacg acaccacgcc gttcgtgaaa atctcgattc
    19741 acgaagtggt taaaacgcta gtcgaagcga tcatcctcgt gttcctggtt atgtatctgt
    19801 tcctgcagaa cttccgcgcg acgttgattc cgaccattgc cgtaccggtg gtattgctgg
    19861 ggacctttgc cgtccttgcc gcctttggct tctcgataaa cacgctaaca atgttcggga
    19921 tggtgctcgc catcggcctg ttggtggatg acgccatcgt tgtggtagaa aacgttgagc
    19981 gtgttatggc ggaagaaggt ttgccgccaa aagaagctac ccgtaagtcg atggggcaga
    20041 ttcagggcgc tctggtcggt atcgcgatgg tactgtcggc ggtattcgta ccgatggcct
    20101 tctttggcgg ttctactggt gctatctatc gtcagttctc tattaccatt gtttcagcaa
    20161 tggcgctgtc ggtactggtg gcgttgatcc tgactccggc tctttgtgcc accatgctga
    20221 aaccgattgc caaaggcgat cacggggaag gtaaaaaagg cttcttcggc tggtttaacc
    20281 gcatgttcga gaagagcacg caccactaca ccgacagcgt aggcggtatt ctgcgcagta
    20341 cagggcgtta cctggtgctg tatctgatca tcgtggtcgg catggcctat ctgttcgtgc
    20401 gtctgccaag ctccttcttg ccagatgaag accagggcgt atttatgacc atggttcagc
    20461 tgccagcagg tgcaacgcag gaacgtacgc agaaagtgct caatgaggta acgcattact
    20521 atctgaccaa agaaaagaac aacgttgagt cggtgttcgc cgttaacggc ttcggctttg
    20581 cgggacgtgg tcagaataca ggtattgcgt tcgtttcctt gaaggactgg gccgatcgtc
    20641 cgggcgaaga aaacaaagtt gaagcgatta ccatgcgtgc aacacgtgct ttctcgcaaa
    20701 tcaaagatgc gatggttttc gcctttaacc tgcccgcaat cgtggaactg ggtaccgcaa
    20761 ccggctttga ctttgagctg attgaccagg ctggccttgg tcacgaaaaa ctgactcagg
    20821 cgcgtaacca gttgcttgca gaagcagcga agcaccctga tatgttgacc agcgtacgtc
    20881 caaacggtct ggaagatacc ccgcagttta agattgatat cgaccaggaa aaagcgcagg
    20941 cgctgggtgt ttctatcaac gacattaaca ccactctggg cgctgcatgg ggcggtagct
    21001 atgtgaacga ctttatcgac cgcggtcgtg tgaagaaagt ttacgtcatg tcagaagcga
    21061 aataccgtat gctgccggat gatatcggcg actggtatgt tcgtgctgct gatggtcaga
    21121 tggtgccgtt ctcggcgttc tcctcttctc gttgggagta cggttcgccg cgtctggaac
    21181 gttacaacgg cctgccatct atggaaatct taggccaggc ggcaccgggt aaaagtaccg
    21241 gtgaagcaat ggagctgatg gaacaactgg cgagcaaact gcctaccggt gttggctatg
    21301 actggacggg aatgtcctat caggaacgtc tctccggcaa ccaggcgcct tcactgtacg
    21361 cgatttcgtt gattgtcgtg ttcctgtgtc tggctgcgct gtacgagagc tggtcgattc
    21421 cgttctccgt tatgctggtc gttccgctgg gggttatcgg tgcgttgctg gctgccacct
    21481 tccgtggcct gaccaatgac gtttacttcc aggtaggcct gctcacaacc attggtttgt
    21541 cggcgaagaa cgcgatactt atcgtcgaat tcgccaaaga cttgatggat aaagaaggta
    21601 aaggtctgat tgaagcgacg cttgatgcgg tgcggatgcg tttacgccca atcctgatga
    21661 cctcgttggc gtttatcctc ggcgttatgc cgctggttat cagtactggt gctggttccg
    21721 gcgcgcagaa cgcagtaggt accggtgtaa tgggcgggat ggtgaccgca acggtactgg
    21781 caatcttctt cgttccggta ttctttgtgg tggttcgccg ccgctttagc cgcaagaatg
    21841 aagatatcga gcacagccat actgtcgatc atcattgata caacgtgtaa tcactaaggc
    21901 cgcgcaagcg gcctttttta tgcataaccg acgaacatta aggagtcatt gaaccaccaa
    21961 ctcaggatct catacgaaaa ccagtattaa acacgtataa aattcataaa a
//
LOCUS       DAZUCC010000067        21910 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZUCC010000067 DAZUCC010000000
VERSION     DAZUCC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.067"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            96..1196
                     /gene="chaA"
                     /locus_tag="ACHY09_002204"
     CDS             96..1196
                     /gene="chaA"
                     /locus_tag="ACHY09_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFS3885173.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1601..1708
                     /gene="ldrA"
                     /locus_tag="ACHY09_002205"
     CDS             1601..1708
                     /gene="ldrA"
                     /locus_tag="ACHY09_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS3885174.1"
                     /translation="MTLAQFTVIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(1857..2711)
                     /gene="kdsA"
                     /locus_tag="ACHY09_002206"
     CDS             complement(1857..2711)
                     /gene="kdsA"
                     /locus_tag="ACHY09_002206"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFS3885175.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(2747..3556)
                     /gene="sirB1"
                     /locus_tag="ACHY09_002207"
     CDS             complement(2747..3556)
                     /gene="sirB1"
                     /locus_tag="ACHY09_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFS3885176.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(3560..3952)
                     /gene="sirB2"
                     /locus_tag="ACHY09_002208"
     CDS             complement(3560..3952)
                     /gene="sirB2"
                     /locus_tag="ACHY09_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFS3885177.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(3949..4782)
                     /gene="prmC"
                     /locus_tag="ACHY09_002209"
     CDS             complement(3949..4782)
                     /gene="prmC"
                     /locus_tag="ACHY09_002209"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS3885178.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIHAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(4782..5864)
                     /gene="prfA"
                     /locus_tag="ACHY09_002210"
     CDS             complement(4782..5864)
                     /gene="prfA"
                     /locus_tag="ACHY09_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS3885179.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(5906..7162)
                     /gene="hemA"
                     /locus_tag="ACHY09_002211"
     CDS             complement(5906..7162)
                     /gene="hemA"
                     /locus_tag="ACHY09_002211"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFS3885180.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDGLGLE"
     gene            7376..7999
                     /gene="lolB"
                     /locus_tag="ACHY09_002212"
     CDS             7376..7999
                     /gene="lolB"
                     /locus_tag="ACHY09_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFS3885181.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPSNMELTDGGQRIKLKMDNWIVK"
     gene            7999..8850
                     /gene="ispE"
                     /locus_tag="ACHY09_002213"
     CDS             7999..8850
                     /gene="ispE"
                     /locus_tag="ACHY09_002213"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS3885182.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGAEISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            9001..9948
                     /gene="prs"
                     /locus_tag="ACHY09_002214"
     CDS             9001..9948
                     /gene="prs"
                     /locus_tag="ACHY09_002214"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS3885183.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            10073..11752
                     /gene="dauA"
                     /locus_tag="ACHY09_002215"
     CDS             10073..11752
                     /gene="dauA"
                     /locus_tag="ACHY09_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFS3885184.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGQ
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(11807..12085)
                     /gene="ychH"
                     /locus_tag="ACHY09_002216"
     CDS             complement(11807..12085)
                     /gene="ychH"
                     /locus_tag="ACHY09_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFS3885185.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            12363..12947
                     /gene="pth"
                     /locus_tag="ACHY09_002217"
     CDS             12363..12947
                     /gene="pth"
                     /locus_tag="ACHY09_002217"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS3885186.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEIWFTDGLTKATNRLHAFKAQ"
     gene            13064..14155
                     /gene="ychF"
                     /locus_tag="ACHY09_002218"
     CDS             13064..14155
                     /gene="ychF"
                     /locus_tag="ACHY09_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS3885187.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEEREEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            14346..14522
                     /locus_tag="ACHY09_002219"
     CDS             14346..14522
                     /locus_tag="ACHY09_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885188.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(14559..16478)
                     /gene="dhaR"
                     /locus_tag="ACHY09_002220"
     CDS             complement(14559..16478)
                     /gene="dhaR"
                     /locus_tag="ACHY09_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFS3885189.1"
                     /translation="MSSAFNNDGRDISPLIATSWERCIKLMKRDTWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRACALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            16706..17776
                     /gene="dhaK"
                     /locus_tag="ACHY09_002221"
     CDS             16706..17776
                     /gene="dhaK"
                     /locus_tag="ACHY09_002221"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS3885190.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRSDAPVAD
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            17787..18419
                     /gene="dhaL"
                     /locus_tag="ACHY09_002222"
     CDS             17787..18419
                     /gene="dhaL"
                     /locus_tag="ACHY09_002222"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS3885191.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSIPAALEAASS
                     VAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            18430..19848
                     /gene="dhaM"
                     /locus_tag="ACHY09_002223"
     CDS             18430..19848
                     /gene="dhaM"
                     /locus_tag="ACHY09_002223"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS3885192.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPSYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            20169..21866
                     /gene="treA"
                     /locus_tag="ACHY09_002224"
     CDS             20169..21866
                     /gene="treA"
                     /locus_tag="ACHY09_002224"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFS3885193.1"
                     /translation="MKSPAASRPLKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQNEAEPTP"
BASE COUNT         5335 a         5658 c         5503 g         5414 t
ORIGIN      
        1 ttcgtaaaat gttaccgata tccagacact ttctgttatt tttcctacct attttttaca
       61 ttttcgataa attcatctgc aaagggataa ccataatgtc aaatgctcaa gaggcggtaa
      121 aaacccgcca caaggagact tcgcttattt tcccggttct ggcgctggta gtgctgttcc
      181 tgtggggaag cagccagaca ctaccagtgg tcattgccat caatcttctt gctcttattg
      241 gtattttaag tagcgccttt agtgttgtcc gtcatgcgga cgtattagcc catcgcctgg
      301 gagagcctta cggttcgctt attcttagcc tttcagtggt tattcttgaa gtcagtttga
      361 tttcagcttt aatggcaacc ggcgacgccg cgccaacgct aatgcgtgat acgctctatt
      421 caatcattat gattgttacc ggtgggctgg ttggcttttc attattgttg ggcggacgca
      481 agtttgccac ccaatatatg aatctgtttg gtatcaagca gtatttaatt gcgctgttcc
      541 ccctggcgat aatcgtactg gtatttccaa tggctctgcc tgcggcgaat ttttcaaccg
      601 gtcaggcgtt actggtagca ttaatttctg cggcaatgta tggcgtattt ttgctgatcc
      661 agaccaaaac gcatcaaagt ttgtttgtct acgagcacga agatgacagt gatgacgacg
      721 acccgcatca cggtaaaccg tctgcccata gcagcctgtg gcatgctatc tggttgattg
      781 tccatctgat tgccgttatt gcggtgacca aaatgaacgc cagcccgctg gagacattgc
      841 tcgacagcat gaatgcccct gtcgccttta ctggcttcct ggtggctctg ttgattctgt
      901 cgccggaagg tttaggtgcg ttaaaagcag tgttgaacaa ccaggtccag cgcgcgatga
      961 atctgttctt tggttcagtg ttagcaacca tttcgcttac cgtacctgtc gtcacgctaa
     1021 ttgcctttat gacgggtaac gaattgcagt ttgcacttgg tgcgccagaa atggtggtga
     1081 tggtggcctc tttagtgctg tgtcatatct ccttctccac cggacgtact aacgtgctca
     1141 atggcgcagc gcatctggca ctgtttgccg cctatttgat gacgatattt gcctgacagt
     1201 tatatctcct ccgatgacgg aaatcggggg agataatata aattcctgct tattgtattt
     1261 cttcatacta cctggacagg ttgttaatca gaattgcgag cctgaaccac aacctgcacg
     1321 aaacgactgt aacagctgtt gaaaaaccgg aatgcactct tcattgcaaa catttcaggg
     1381 atgaagtgaa gatgaattga gtcgtagtat ccacaccaag ccggcgttat ccggtgaggc
     1441 gcaatgttgc gggggcttta tccctggtgg cattggttgc tggaaagaga aaacccccgc
     1501 acgttgagag gtataacctg acaacagaac gggggctaat cttgaaccag aacaattcaa
     1561 gaatagccgc aaaatgttgt cattacaaca aggcggctat atgacgctcg cgcagtttac
     1621 cgtgattttc tggcacgacc tggcagcacc gatcctggcg ggaattatta ccgcagcgat
     1681 tgtcggctgg tggcgtaacc ggaagtaagg cgaaaacggg cgtcaggctg ccgtgatggc
     1741 aatatgcgcc cgaaaccggc cgcaggggta acagcctgcg gccctttttg tatccggccc
     1801 ccatgaaaaa agccttaacg cagaacgcta aggctttagt tttcaggcaa aaaagattac
     1861 ttgctggtat ccagttcttc gaaacctttc accaagtcat caatcgcttt catctgcttg
     1921 aggaacggtt ccagtttagc cagcggcagc gcggatggac catcacattt cgcatgttcc
     1981 ggatccggat gcgcttcaat aaacagcccc gccagaccta ccgccatacc ggcacgtgcc
     2041 agctcagcca cctgagcacg acgaccgccg gaagctgcgc caaacggatc gcggcattgc
     2101 agtgcgtggg tcacgtcgaa aatcaccggc gaattaccag acactttctt catgatgctg
     2161 aagcccagca tatcgacaac caggttgtca tagccgaagt tagcaccgcg atcgcaaaga
     2221 atcacttttt cgttgccgcc ttctttaaat ttatcaacga tattacccat ctgtcccggg
     2281 ctaacaaact gcggtttctt gacgttaatt accgcaccgg ttttcgccat ggcttccacc
     2341 aggtcagtct ggcgagcaag aaacgccggc aactgaatca catccacgac atccgcaacg
     2401 ggctgtgcct gacttggttc gtgaacgtcg gtgataattt tcacaccaaa agtctgcttc
     2461 aactcctgga agattttcat cccttcttcc aggcccggtc cacgataaga gtggatggag
     2521 gagcggttgg ctttgtcaaa agaggctttg aacacgtaag ggatacccag tttctgggtc
     2581 acggttacgt agtgctcgca aatgcgcatc gccagatcgc gagattccag cacgttcata
     2641 ccgccaaaca gtacgaacgg caggtcattt gctacgttga tgtcgccaat gctaaccact
     2701 ttttgtttca taggatcgcc ttaatcttga gtaaaatgtc gattaattaa tgcagcacaa
     2761 tatgtttatg cgcgatgtta tttatttgcg cacggatcat ttcgctgatc gggtcttccg
     2821 gacactgttc aacgaaataa cttaaatcgt tcaacgcaac gtgttcgcaa tccagttgcg
     2881 cataaatcaa cccgcgatcg cgaatttcat agggatcttc cgggttgaat tgtaataaag
     2941 cttcgctggt gcgtaacgcc agctccatct gattctcttc catcaacgaa gctttgagtg
     3001 tatccagcaa tttgcggatt acctcaatgt tatcagcttc atcaaggtct tcataaaaca
     3061 gttccgccga cgggctgata tttcccttta accatacgtc cagcatatgt tcgcttaacg
     3121 attcaccgtt aaaaggatta atcagccaaa tttcgccatc cggacattca atgcgcaata
     3181 tcagctgcgt agggaaaatc accggcagca gcggcaaatc aagacgattc gcgacccata
     3241 ataaaacggc acctaatgat accgcactgc cctgtcgatt ctttaacacc tggtccagcc
     3301 ataatgcatc ggaaagacga taaacgccgc gtgaggcttt aaatccccag tcgccgtaaa
     3361 acagcgcgat cagtttttcc agctgctctt ctaaaggcag aagctggctg atttcttcct
     3421 tcgccagact aacgagacgc tccagttcgt cgtaaacatc ttgcgaggga aaatcgcggc
     3481 ggattgcttc gcaagccagg atcatgcctt cgcacaatgg cgctttatta aattcgaaat
     3541 cagctaacga tctcatgact taccccagta acggtacttt tgtggtggcg agtttaatga
     3601 tgatgtacag caccaccaac gccagcggga aggcaataat gcgcgcctgc tgactgtgca
     3661 tacgacgata atcgagtgca ataaaaccca aaacgatata aatgataact ccaaacagct
     3721 tttcagtcag ccactgtgcc tgcccggaaa atggcaggat gtgcgcttta gccatcaacg
     3781 caatgccgct taacaataac agcgtatcga caaccggcgg cacaatgcgc gtccagcgcg
     3841 caaatgcctg tggatgcttc tgataacgta gccagaaacg taaggttaat agcccaactg
     3901 aaagcgcgat actaataaga tgaacactaa gcagtgtaga aaaacttgtc attgataata
     3961 gcggccgagc gttacgcgct cgttatcacc atagtcccga caggtttcga cgtcatgata
     4021 tcccgcgtgg ataaatgcct gtcgcaccgc ttcgccctgc tgccaaccat gttccagaag
     4081 cagaaagccg ccggatacca gcgcgttacg cgactgttcg atgatatgca cgatgtctgc
     4141 cattccactg tctgccgcaa ccagcgcagt gagcggctca aagcggacat cgccttgttg
     4201 aagatgtggg tcctgctcgt caatatacgg cggattactg acaatcatcg caaactgctg
     4261 cccggctagc gcgctaaacc agtcgctttg cagaatatgg atgtttttga tcgccagatt
     4321 ctgggcatta cgttgcgcca gggcgacagc atctggcata cgatctacag cggtaatttc
     4381 gcagtccgga cgctcgctgg ccagcgccag cgcaatcgcc ccggtacccg tcccgagatc
     4441 gagaatacgg caaggttgtt caggcaaccg cgccagtgcc tgctccacca gacactccgt
     4501 atccgggcgc ggaattaagg tcgctggcga aacaaataac ggcaacgacc agaattctcg
     4561 caccccggtt aaatgagcaa tgggttcacc atcgcgacga cgcgtcagta gcgcatcaag
     4621 ttgctgacat tgttcgtcag tcagctgcgt ttcaccaaag gcgagaataa aagtacgccc
     4681 tctgccggta acatgctcca gcaggatttc agcatcacgc cgcgggcttt cgctcgcctg
     4741 aagttggctt attgcttcac gtaaccagtg ttgatattcc attattcctg ctcggacaac
     4801 gccgccagtt ggtcggcctg atgttcctgg ataatcggtt caatcagcat atccagctta
     4861 ccttccatca cttcatccag gcggtagagc gtcaggttga tgcggtgatc ggtaacgcgc
     4921 ccctgtggga agttgtaagt acggttacgg tcgctgcgat cgccactccc cagcaggtta
     4981 cgacgggtag acgcttcggc ctgttgccgt tttgccattt ctgcagcgtg gatgcgagca
     5041 ccgagtacag aaagtgcttt agctttgttt ttatgttgtg aacgttcgtc ctgacattca
     5101 acaacaatcc cggtcggcaa gtgagtaata cgaattgccg aatcggtggt gttaacgtgc
     5161 tgaccacccg cccctgacga gcggaaagta tcaatgcgca aatctgctgg gttgatgtcc
     5221 ggcagttctg cgtcaggcag ttctggcatt accgcaacgg tacaagcgga agtatgaatt
     5281 cgaccctgcg attccgtagc aggcacacgc tgcacgcgat gaccgccaga ttcgaatttc
     5341 aaacgaccat atacaccatc accgctaatt ttggcgatga tctctttata accaccatgt
     5401 tcaccctcgc tggcgctcat gatttctaca cgccagcggc gggcttcggc gtaacggctg
     5461 tacatacgga acagatcgcc agcgaagagc gccgcttcgt cgccgccggt tccggcacgg
     5521 acttcgagga aggcgttacg ttcgtcatca ggatcttttg gtagtaacag aacctgtaat
     5581 tgctgttcca gttgctcgct tttttcttta gcttcgcgca gttcatcctg cgccatctca
     5641 cgcatttcag gatcgtcgag catcatctgt gcggtttcga tatcttcctg aacctgttgc
     5701 cagtcggtaa aacagcgcga aacatcactt aactgcgcat attcgcgtga taatgcgcga
     5761 aaacgttcct ggtcggcgat agtttgcgcg tcacccagca acgcctgaac ttcttcatgg
     5821 cgttcatgca gggcttccag tttggcaacg atagaaggct tcataggcgt aaatgcaccc
     5881 tgtaaaaaaa gaaaatgatg tactgctact ccagcccgag gccgtcgcgc agaatattca
     5941 ggcgttcgtt atccccgtca cgggcggcct gttgaagtga tttcgttggc gcatggatca
     6001 ggcggttagt cagtttccat gccagatcct gcataatggc ttgagcgtca ccgccctgct
     6061 caagggccgc taacgctttg gcagttaact catcgcgaac gtgctctgcc tggctgcgat
     6121 actcgcgaat ggtttcgctg gcgctttgtg cacgcagcca cgccataaat tcgctggttt
     6181 cctgagcgac aatagtttcc gcctcaaccg ctgcggcttt acgctgcgcc aggttgtgcg
     6241 aaatgatgct ttgcagatcg tccacgctat aaagataagc attcgccagt ttgccaactt
     6301 ccggctcaac atcgcgcgga acggcaatat ccaccaacag cattggttgg ttgcgacggc
     6361 tttttaatgc gcgctccacc atgcctttcc cgataatcgg taacgggctg gcggtggaac
     6421 tgatgatgat atcggcttcg cgcagacgtt cgtcgatatc actcagggca ataacttccg
     6481 cgccgacttc atctgccaga atttgggcac gttcgcgagt gcggttggcg ataatcatct
     6541 tctgtacttt gtgttcgcgc agatgacgcg ccaccagctc gatggtttcg cccgcaccca
     6601 ccagcagtac cgtcaccgta gagagcgatt caaagatctg ccgcgccagc gtacaagccg
     6661 caaaagcgac agaaacagcg ctggcaccga tatctgtttc agtgcgaaca cgtttcgcta
     6721 cagagaaaga tttctgaaac atgcgttcca gttcgctggc cttcatatgg cctttttgcg
     6781 aatcggcaaa cgctttttta acctgaccga ggatctgcgg ctcccccaaa accagtgaat
     6841 ccaggccgct ggcaacacgc attaaatggc taaccgcgtc gttatcctga tgccagtaga
     6901 ggcttttacg caggtcttct tcattaagat tgtgataatc gcaaagccag cggattaacg
     6961 cctcttgcag gttatcctgc tcttcaacgc taagataaag ttccgtgcga ttgcacgtcg
     7021 acagcaccac gccgccctgc accatcggct gcgcaagcag gctgtcaagc gcctgatcga
     7081 gcttatccgg cgaaaacgat acacgttctc gcagcgatac aggtgccgtt ttatggttga
     7141 taccgagtgc taaaagggtc atgtctgcgg gaaataatac caacgttgat agggttagtc
     7201 tgcttgcatc atacaggatg cgtaagatca ataaaagaga gcgccccctt ttggagtaat
     7261 tgccgaaagc cgttagattc tggcaattaa gacaacttga acatagacga tagcggacgg
     7321 taacgctagc attaagggtt ataactgcaa cgtatctcaa ggacttgtca tcactatgcc
     7381 cctgcccgat tttcgtctta tccgcctact accgctggct gctcttgtgc tcactgcctg
     7441 ttccgttacc acgcccaaag gtcctggcaa aagcccggat tcgccacaat ggcgtcagca
     7501 tcagcaagac gtgcgcaatc ttaatcagta tcaaactcgc ggcgcgttcg cttatatttc
     7561 tgaccaacaa aaagtgtacg cccgtttctt ctggcagcaa actggccagg atcgctaccg
     7621 tctgctgctg actaacccat tgggcagcac ggaactggag ctgaatgctc aaccgggtaa
     7681 cgtgcagtta gtcgacaata aaggccagcg ttataccgcc gatgacgccg aagagatgat
     7741 tggcaaattg accggaatgc ccattccact caacagctta cgccagtgga ttttaggttt
     7801 gccgggtgat gcgactgact acaaactgga cgaccagtac cgccttagcg aaatcaccta
     7861 cagccagaat ggcaaaaact ggaaagttgt ttacggtggt tatgacacaa aaactcaacc
     7921 tgcgatgcca tccaatatgg aactcaccga tggtggtcag cgcatcaagt taaaaatgga
     7981 taactggata gtgaaataat gcggacacag tggccctctc cggcaaaact taatctgttt
     8041 ttatacatta ccggtcagcg tgcggacggt taccacacgc tgcaaacgct gtttcagttc
     8101 ctggattacg gcgacaccat cagcattgag cttcgtgacg atggggatat tcgtctgtta
     8161 acacccgttg aaggcgtgga acatgaagat aacctgatcg ttcgcgcagc acggttgctg
     8221 atgaaaacag cggcagacag cgggcgtctt cctacgggaa gcggtgcaga aatcagtatt
     8281 gacaagcgtt tgccgatggg cggcggtctg ggtggtggtt catccaatgc cgcgacggtg
     8341 ctggtggcat taaaccatct ctggcaatgc gggctaagca tggatgagct ggcggaaatg
     8401 gggctgacgc tgggcgcaga tgttcctgtc tttgttcggg ggcatgccgc gtttgccgaa
     8461 ggcgttggtg aaatactaac gccggtggat ccgccagaga agtggtactt ggtggcgcac
     8521 cctggtgtaa gtattccgac accggtgatt tttaaagatc ctgaactccc gcgcaatacg
     8581 ccaaaaaggt caatagaaac gttgctaaaa tgtgaattca gcaatgattg cgaggttatc
     8641 gcaagaaaac gttttcgcga ggttgatgcg gtgctttcct ggctgttaga atacgccccg
     8701 tcgcgcctga ctgggacagg ggcctgtgtc tttgctgaat ttgatacaga gtctgaagcc
     8761 cgccaggtgc tagagcaagc cccggaatgg ctcaatggct ttgtggcgaa aggcgttaat
     8821 ctttccccat tgcacagagc catgctttaa gccgggcaag ctgagtttcg gtgacaacgt
     8881 caccttgttc cagacgttgc atcgcgctct ttaatacacc gcctggaaag aatcatgcct
     8941 ggcccgcaca gttttcggca gattctttcc accaatggac gcatgcctga ggttcttctc
     9001 gtgcctgata tgaagctttt tgctggtaac gccaccccgg aactagcaca acgtattgcc
     9061 aaccgcctgt acacttcact cggcgacgcc gctgtaggtc gctttagcga cggcgaagtc
     9121 agcgtacaaa ttaacgaaaa tgtacgcggt ggtgatattt tcatcatcca gtccacttgt
     9181 gcccctacta acgacaacct gatggaatta gtcgttatgg ttgatgccct gcgtcgtgct
     9241 tccgcaggtc gtatcaccgc tgttatcccc tactttggct atgcgcgcca ggaccgtcgc
     9301 gtccgttccg ctcgtgtacc aatcactgcg aaagtggttg cagacttcct ctccagcgtc
     9361 ggtgttgacc gtgtgctgac agtggatctg cacgctgaac agattcaggg tttcttcgac
     9421 gttccggttg ataacgtatt tggtagcccg atcctgctgg aagacatgct gcagctgaat
     9481 ctggataacc caattgtggt ttctccggac atcggcggcg ttgtgcgtgc ccgcgctatc
     9541 gctaagctgc tgaacgatac cgatatggca atcatcgaca aacgtcgtcc gcgtgcgaac
     9601 gtttcccagg tgatgcatat catcggtgac gttgcaggtc gtgactgcgt actggtcgat
     9661 gatatgatcg atactggcgg tacgctgtgt aaagctgctg aagctctgaa agaacgtggt
     9721 gctaaacgtg tatttgcgta cgcgactcac ccgatcttct ctggcaacgc ggcgaacaac
     9781 ctgcgtaact ctgtaattga tgaagtcgtt gtctgcgata ccattccgct gagcgatgaa
     9841 atcaaatcac tgccgaacgt gcgtactctg accctgtcag gtatgctggc cgaagcgatt
     9901 cgtcgtatca gcaacgaaga atcgatctct gccatgttcg aacactaatc gaacccggct
     9961 caaagacccg ctgcggcggg tttttttgtc tgtaatatcc atttgtatga cctatgcctc
    10021 cttcacctgc catttagttg acagatgatg cgctcacgga tgaaacatta ttgtgaacaa
    10081 aatattttcc tcacatgtga tgcctttccg cgctctgatc gacgcttgct ggaaagaaaa
    10141 atatactgcc gcacggttta cccgtgacct gattgccggg ataaccgtcg ggattattgc
    10201 tatcccgctg gcaatggcgt tggctattgg tagtggtgtg gcaccccagt acggtttata
    10261 taccgcagct gttgcgggga ttgtcattgc tctgacgggt gggtcacgct ttagcgtttc
    10321 cggtccgact gcggcatttg tggtaattct ctatcccgtt tcgcaacagt ttggacaggc
    10381 aggactgctg gtcgcgacac tcatgtcagg tgtctttttg atcctgatgg ggctggcacg
    10441 ttttggacgc ttgattgaat atattccggt ttccgttacc ttaggtttta cttccggtat
    10501 cgggatcacc atcggtacca tgcagattaa agattttctc ggtctgcaaa tggcccatgt
    10561 ccctgaacat tatctacaaa aagtcggcgc attatttatg gcgttgccga ccattaatgt
    10621 gggtgatgct gccattggca ttgtgacgct tggcattctg gttttctggc cacgtctggg
    10681 cattcgttta cccggtcacc ttccggcctt gctggctggt tgcgcggtga tggggattgt
    10741 taacctgctc ggcggacatg ttgctaccat cggttcgcaa ttccactacg ttctggccga
    10801 tggttctcag ggtaacggta ttccgcaact gctgccgcaa ctggtactgc cgtgggacct
    10861 gcccaattcc gaatttaccc tcacctggga ctccattcgc actctgcttc cggcagcatt
    10921 ttcgatggcg atgttagggg caattgaatc actgctctgc gccgtggtgc tggatggtat
    10981 gaccgggacg aaacacaaag cgaacagtga actggttgga cagggactgg ggaatatcat
    11041 cgctccgttc tttggtggta ttaccgctac agctgccatc gcgcgttctg ccgctaacgt
    11101 ccgtgccggg gcaacttccc ctatctcggc ggtgatccac tctattctgg ttattcttgc
    11161 cctgctggta ctggcaccgc tgctctcctg gctgcctctt tccgccatgg ctgccctgct
    11221 gttgatggtg gcgtggaaca tgagtgaagc gcacaaagtg gtcgacttgc tgcgtcatgc
    11281 gccgaaagat gacatcatcg tcatgctgct gtgcatgtcg ctgaccgtgc tgtttgatat
    11341 ggttattgcc atcagcgtgg ggatcgtgct ggcatcgcta ctgtttatgc gtcgtatcgc
    11401 acgtatgact cgcctggcac cggtagtcgt agacgttcca gacgatgttc tggttctgcg
    11461 cgttattggc ccgctgttct ttgctgccgc tgaaggcttg ttcacggacc tggagtcacg
    11521 tcttgaaggc aaacggattg tgattctgaa gtgggatgcc gttccggtac ttgatgctgg
    11581 tggtcttgat gcgttccagc gttttgtgaa gcgtctaccc gaaggatgtg aactgcgcgt
    11641 gtgcaacgtg gaattccagc cactgcgcac tatggctcgc gcaggcattc aaccgatccc
    11701 gggacgcctg gcgttcttcc cgaatcgtcg cgcggcgatg gcggatttat aagtacgaaa
    11761 ttgaccagtc agcgcaaact gactggtcag caaaccgcat tatttgttag ctatgacggc
    11821 gattatcgct acggcggcaa cgtttgtcat agtggcgaac ccaccagtaa cggtcgcaca
    11881 cctgttcatg cccgccaaca cgggcacccg ccagccagag aatggcaccg acgaaaatac
    11941 ttagcactgc accatgtgcg aaatactggg gcatattaaa ctgtggtaac tggttgagga
    12001 ttgaataccc cacgccgacc accattacca ccagacccaa ccccatgagc acgttaccga
    12061 gtaacgaagc gtttttgcgt ttcatatgtc acctccggaa ctttctgggt tgtaacaggg
    12121 aatacccctc ttccttatgt gtaaagtata gacaacaccc tgtgggttat gtgcgggcgt
    12181 gatcacaatt acaaccctta tttcaacaaa actttacaaa taaacgcctg aacaccttga
    12241 ctttctaata gtccacttag gcaattattg cgttatctgc atttgatacg cagttttttg
    12301 tcaagcgggg ccgcaaacag taaactacgc gccagttatg tacacactca ggacaaaaaa
    12361 acgtgacgat taaattgatt gtcggcctgg cgaaccccgg cgctgaatac gccgcaacgc
    12421 gacataatgc tggtgcctgg ttcgttgact tactggcaga gcgattgcgc gctccgctgc
    12481 gcgaagaggc taaattcttt ggttatactt cgcgagtcac ccttggaggc gaagatgtcc
    12541 gcctgttagt cccgactaca tttatgaatc tcagcggcaa agccgttgcg gcgatggcca
    12601 gttttttccg cattaatccg gacgagattc tggtggccca cgacgaactg gatctgcctc
    12661 ctggcgtcgc caaatttaag ttgggcggtg gccatggtgg tcacaatgga ctgaaagaca
    12721 tcatcagtaa attgggtaat aaccctaact ttcaccgttt acgcatcgga attggtcatc
    12781 caggcgataa aaataaagtt gtcggttttg tgttaggcaa accgcctgtt agtgaacaga
    12841 agttaattga tgaagccatc gacgaagcgg cacgttgtac tgaaatatgg tttacagatg
    12901 gcttgaccaa agcaacgaac cgattgcacg cctttaaagc gcaataagtc gttgtctgcg
    12961 gcatttttgc cgagtgccgt gtataatagg caaagttatt tccatttctg caatctgtta
    13021 gcaataacag gttgattatt aagatattaa ggtgatttaa atcatgggat tcaaatgcgg
    13081 tatcgtcggt ttgcccaacg tcgggaaatc taccctgttc aacgcgctga ccaaagccgg
    13141 tattgaagcg gccaactttc cattctgtac cattgagccg aacacgggcg tcgtaccaat
    13201 gcctgaccct cgcctggatc aactggctga aatcgtaaaa ccgcagcgta cgcttcccac
    13261 taccatggaa tttgtcgata tcgccggtct ggtaaagggc gcgtcgaaag gtgaaggtct
    13321 gggtaaccag ttcctgacca acattcgtga aaccgaagcg atcggccacg ttgttcgctg
    13381 ctttgagaat gacaatatca ttcacgtttc cggtaaagtt aacccggcgg acgatattga
    13441 agttatcaat accgaactgg cactggcgga tctcgacacc tgcgagcgcg cgattcaccg
    13501 cgtacagaag aaagccaaag gcggcgataa agacgcgaaa gccgaactgg cagtactgga
    13561 aaaatgcctt ccacagctgg aaaacgcggg catgctgcgc gcgctggatt taagcgcaga
    13621 agaaaaagcg gctattcgtt acctgagctt cctgacgctg aaaccgacga tgtacatcgc
    13681 caacgtcaac gaagacggtt ttgaaaacaa cccgtatctt gaccaggtgc gtgaaatcgc
    13741 ggcgaaagaa ggttctgttg tggttccggt ttgtgcagct gttgaagcag acattgccga
    13801 actggacgat gaagaacgtg aagagtttat gcaggaactg ggactggaag agccgggcct
    13861 gaaccgtgtg atccgtgccg gttataaact gctgaacctg caaacttact tcaccgctgg
    13921 ggtgaaagaa gtgcgcgcat ggaccatccc cgtcggcgca actgcaccgc aagcggctgg
    13981 caaaatccat accgatttcg aaaaaggctt tatccgcgca cagactatct cgtttgaaga
    14041 tttcatcact tacaaaggtg aacaaggcgc gaaagaagca ggcaaaatgc gtgcagaagg
    14101 taaagattac atcgttaaag atggcgatgt gatgaacttc ctgttcaacg tctaaattcc
    14161 acgtaatacc ctgacggtta ataatgagcc attatgtgag tcgcacataa tggctttttt
    14221 acgcctgcta cgcggcttct ttcaattctg agttatcgaa aatcataatc agactcctgg
    14281 caatattaac aatgtgcctt ttatgtgata acaatcacag tgcattaacc gttcaagagt
    14341 ttgtcatgaa aaaaacattt accttcagcc tgttagcaat tgtctttgcg agcctgttaa
    14401 gtgcttgtgt tccccaccat catcaccgcc acagcgatgg ccctcgtggt cctgcgccct
    14461 ccggtaagcc aatgccacct tcaaatggtg gaccaaatca cggcagtcat ggtcgctatt
    14521 aattagcaat tcatagcggg aaagtacata ctctcccgct atccacggcg cttaaactgc
    14581 cctgcatcaa tgccatgttg cttcattttc cgccacagcg tagtgcggcc gattccaagt
    14641 aaagccgaca tttcctgaat gcgaccgcct gtgacctggg ctgcgttaat aattgcctct
    14701 ttttcaactt ccgcaaatga cagactggtg gaaaggcgag tggcgctgac atcatctgtc
    14761 gcctgctcgg taaagagatg ttcaggtaaa tcactgacgc gaatgcgccc gttatcacta
    14821 ctcagagcaa gattctcgat gacgctgtaa agttcaaaat cgttgccagg ccatgcacag
    14881 gaaaccaggc gagcgagggc atcgtcatca attttcagcc gcgtagagaa gcgtttttca
    14941 agactgtgta atttgttatt caccagcgcc ggaatgctgc cacgccgcat acgcagaggc
    15001 gggatggtaa tttcaaatgc atgcagcgcg taatacagct ggcgactaaa acgattttgt
    15061 tccaccagca ttgccaggtc cgcggtcgtt gtggcaatca ctttgacatc aatgggtatt
    15121 aaacgccgcg catccagtcg cgtgataaca ccctgcttga taacctgaag caaagcagac
    15181 tgtaactcca ccgccagata ttcaatcttt tcaagaaaca gcgtgccgcc gtgcgccagt
    15241 tccagtcgac tcaaacgacc attttcattg tccgtgcgat cgccgccaat aaattcttcc
    15301 gccagcgccg catcaccata taactcacaa ttgacggcga tataaggccc tgctgcacgc
    15361 tcactttcat tatgaattgc ctgacttagc agtgccttgc ccacgccctc ttctccacaa
    15421 agcaggacag gaaagctact gcgcgccgcc tggcgaccaa aatgaatcaa acggcgggtt
    15481 tgcggatcct cttgtggcat atgagcgaaa gtatggctga cttttcctaa ttgactggtc
    15541 attaactgcc gcatctgttc cacaggatgg agcaacaaaa taaagctggt tccctgcgtt
    15601 tctattatcg gtttaagggt tatcaccgca tcaataaact ggtgctggct ttcaaaggtt
    15661 gcttctacgt gtttgagcgg atgtgcctgt tttattgctt gttgcaatac ggcgggtaac
    15721 gttaagagtt cagtgattgc ccgtccctga cttgccgtcg cgtcaaggcg taagacccgc
    15781 gccgcctggg cattaataaa ttgcaaatta ccctgctcgt cccagctaat cacgccatca
    15841 tccatacttt ctaacagggc attaagttga tttaaatgac ggttagtttc agcgagcaaa
    15901 ctgtccgtca gcagtaaatt tccgacctcg cgtgcgattg ccaacgtcaa cggcaaatca
    15961 gctgcggtag tttgctcaac cggacacgcc agcgctattg ttcccgtcaa tcggcccttg
    16021 ctgtcaaaca acggcgttgc acaaaaggcc cagttccaga gtgcctgttt gaaatgttga
    16081 tcggccatcg ttttcacggc ctgaccagag atagccgcta acgatagcgc acaagtacca
    16141 ataattccct cggcgcaata cgtgccgtca ttgaatccca gtgcacttag ttgctgcaag
    16201 gtttgcggat cgccattacg gctgagaatg caggcggttt catcgaggat aagcagcgca
    16261 cacgctcgcg gtgccatata ttcccaggca tcttccagcg cagcctgccc gagcgtcagc
    16321 atcgctttct tacgccgata aatagacgca aatgtcacgc cctgggcctg gtgtggtacg
    16381 ttccatgtat cccgtttcat cagcttaatg catcgctccc aggaggttgc aattaaggga
    16441 gatatgtccc gaccatcgtt gttaaaagcg ctactcatat cccgcatccc ttatgacaaa
    16501 aggtgttccg gctcgcaatt ttaagtatta atacacgtaa aagtttcaat atggaacatt
    16561 cattaaagaa tatgtttcac tatgaaacat aatttgggct aattttcttt tcccttgcct
    16621 gatgcacaat ggattcatcg gcagtcagtg gtcgccgtgt cgttgaacat catccatgcc
    16681 ctaccgtaat tgctggagca aaataatgaa aaaattgatc aatgatgtgc aagacgtact
    16741 ggacgaacaa ctggcaggac tagcgaaagc gcatccatct ctgacactgc accaggatcc
    16801 ggtgtatgtc acccgatctg atgcccccgt tgcagataaa gtcgccctgc tgtcgggtgg
    16861 cggcagcgga cacgagccga tgcattgtgg ttatatcggt caggggatgc tttcgggggc
    16921 ctgtccgggc gaaattttca cctcaccgac gcccgataaa atctttgaat gcgccatgca
    16981 aattgatggc ggcgaaggtg tactgttgat tatcaaaaat tacaccggcg atattcttaa
    17041 ctttgaaaca gcgaccgagt tactgcacga tagcggcgta aaagtgacca ctgtggtcat
    17101 tgatgacgac gttgcggtaa aagacagtct ttataccgcc gggcgacgcg gcgttgccaa
    17161 caccgtatta attgaaaaac tcgtaggcgc agcggccgag cgtggcgact cactggacgc
    17221 ctgtgcggaa ctggggcgta agttgaataa tcaaggccac tcaataggta tcgctctcgg
    17281 tgcctgtacc gttcctgccg cgggcaaacc ttcttttacc ctggcggaca atgagatgga
    17341 gtttggcgtc ggcattcatg gtgagccagg tattgaccgc cgtcccttct cttcccttga
    17401 tcaaaccgtc gatgaaatgt tcgacaccct gctggaaaat ggctcatacc atcgcacttt
    17461 gcgtttctgg gattatcaac aaggcagttg gcaggaagaa tcacaaacta aacaaccgct
    17521 ccagtctggc gatcgggtga ttgcgctggt taacaatctt ggcgcaactc cgctttctga
    17581 gctgtacggc gtctataacc gcctgaccac acgttgccag caagcgggat tgactatcga
    17641 gcgtaattta attggcgcgt actgcacctc actggatatg accggtttct caatcacctt
    17701 actgaaagtt gatgacgaaa cgctggcact ctgggacgcc ccggtccaca ccccggccct
    17761 taactggggt aaataaggag atagcaatgt cactgagcag aactcaaatt gttaactggc
    17821 tcactcgttg tggcgatatt ttcagcaccg agagcgagta tcttaccgga ctggatcgcg
    17881 aaattggcga cgctgaccac gggctaaata tgaaccgagg ctttagcaaa gtggtggaaa
    17941 aactccctgc tatcgcagat aaagatatcg gtttcattct caagaatacc ggtatgacgc
    18001 tactttccag cgtcggtggt gccagtggtc cgcttttcgg taccttcttt atccgcgccg
    18061 cacaggcgac ccaggcacgg caaagcctga cgctggaaga gctttatcag atgttccgcg
    18121 atggcgcgga cggcgtaatc agtcgcggga aagccgaacc tggcgataaa accatgtgtg
    18181 atgtgtgggt gccggtggtg gaatcgttac gtcagtccag cgagcaaaat ctctctattc
    18241 cggcggcgct cgaagctgcc agtagcgtcg ccgaatccgc tgcacaaagt acgattacga
    18301 tgcaagctcg caaaggccgt gccagttatc tcggtgaacg cagtattggt caccaggacc
    18361 ccggtgcgac ctcggtgatg tttatgatgc aaatgttggc gttagccgca aaagagtaag
    18421 gaattggtga tggtaaacct ggtcatagtt tcacatagca gccgactggg agaaggcgtc
    18481 ggtgaattag cccgtcagat gttaatgagt gataattgta aaatcgccat tgccgcggga
    18541 attgacgatc cacaaaatcc cattggtacc gatgccgtca aagtgatgga ggccatcgaa
    18601 tctgttgccg ataccgacca tgtgctggtc atgatggata taggaagcgc attattgagt
    18661 gctgaaactg cgctggaatt gctggctccc gagatcgccg caaaagtacg tttgtgtgct
    18721 gcgccgttgg tcgaaggtac actggcagca acggttagcg cggcctcggg ggcggagatc
    18781 gacaaagtga tctttgacgc catgcatgcg ctggaagcca aacgtgaaca actgggttta
    18841 ccctcctcca acactgaaat ctctgacaca tgtccttcgt acgatgaaga agcccgttct
    18901 ctggcggtgg tcataaaaaa ccgtaacggc ctgcatgtac gtccggcctc ccggctggtt
    18961 tataccttat cgacatttaa tgccgatatg ttgctggaga aaaacggcaa atgcgtcaca
    19021 ccggagagta ttaaccagat tgcgttacta caagttcgct ataacgatac gctgcgcctg
    19081 attgcgaaag ggccagaagc tgaagaggca ctgatcgctt tccgtcagct ggctgaagat
    19141 aactttggtg aaacggagga agttgctcca cctattctgc gtcccgttcc gcctgtttcg
    19201 ggtaaagcct tttattatca accggtttta tgtacggtac aggcaaaatc aaccctgacc
    19261 gtggatgaag aacaagaacg attacgccag gccattgact tcacgttatt agatctgatg
    19321 acgttaacag cgaaagcaga agccagcggg cttgacgata ttgccgcaat cttttctggt
    19381 caccatacac tgttagatga tccggaactg ctggcggcgg caagcgaact ccttcagcat
    19441 gaacattgca cggcagaata tgcctggcag caagttctta aagaacttag ccagcaatac
    19501 cagcaactgg atgatgaata tctacaggct cgctatattg atgtggacga tcttctgcat
    19561 cgcaccctgg tccacctgac ccaaacgaaa gaagaactcc cgcagtttaa ctcgccaact
    19621 attctactgg cggagaacat ctatccctcc acaatactgc aactggatcc ggcggttgta
    19681 aaaggtatct gccttagcgc cggaagtccg ctatcccaca gcgccctgat cgcccgtgaa
    19741 ctggggattg gctggatttg ccagcagggt gagaaactgt atgcgataca accagaagaa
    19801 acgctaacgc tggacgttaa aacgcaacgt ttcagccgtc agggttaaac caccgttcgg
    19861 atggcatcgt tctgatgcca tccggctatt tactctgcac ggatgatttt taactacctg
    19921 ttatattttt taccaaatca tgatgatttt ttctgcgcta atcgcgaacc caacgccgct
    19981 ggctggctaa caaacagacg ttatttgatc actctatttc gacgttattt atccctttaa
    20041 ttcccttttc taaaatgcct gaccgttcgc agaatgagat ttcgatcatg cagctagtgc
    20101 gatcctgaac taaggttttc tgatacttga ataccgtttt attcggtttc gccaaaggag
    20161 aatgattgat gaaatccccc gcagcttctc gcccgctaaa aatggcgtta attccagcct
    20221 gcatcttttt gtgtttcgct gcgctatcgg tgcaggcaga agaaacaccg gtaacgccac
    20281 agccgcctga tattttatta gggccgctgt ttaatgatgt gcaaaacgcc aaactttttc
    20341 cggaccaaaa aacctttgcc gatgccgtgc cgaacagcga tccgctgatg atccttgctg
    20401 attatcggat gcagcaaaac cagagcggat ttgatctacg ccatttcgtt aacgtcaatt
    20461 tcaccctgcc gaaagagggc gagaaatatg ttccgccaga ggggcaatca ctacgcgaac
    20521 atattgacgg actttggccg gtattaacgc gttctaccga aaataccgaa aaatgggatt
    20581 ctctgttacc gctgccggaa ccttatgttg tgccgggcgg acgttttcgc gaggtatatt
    20641 actgggacag ttacttcacc atgttagggc ttgccgaaag tggtcactgg gataaagtcg
    20701 cggatatggt ggccaatttt gctcatgaaa tagacactta cgggcatatt cccaacggca
    20761 accgcagtta ctatttaagc cgctcacaac cgccattctt tgccctgatg gtagagttac
    20821 tggcgcagca tgaaggcgat gccgcgttga agcaatacct gccgcaaatg caaaaagaat
    20881 atgcttactg gatggacggt gttgaaaacc tgcaagccgg acaacaggaa aaacgcgttg
    20941 tcaaacttca ggatggtacc cttctcaacc gctactggga cgatcgcgat acgccacgac
    21001 cagagtcatg ggtggaagat attgccaccg ccaaaagcaa tccgaatcga cctgccactg
    21061 aaatttaccg cgacctgcgc tctgccgctg cgtctggctg ggatttcagc tcgcgctgga
    21121 tggacaatcc gcagcagtta aataccttac gcaccaccag catcgtaccg gtcgatctga
    21181 acagcctgat gtttaaaatg gaaaaaatcc tcgcccgcgc cagcaaagct gccggagata
    21241 acgcgatggc aaaccagtac gaaacgctgg cgaatgcccg tcaaaaaggg atcgaaaaat
    21301 acctgtggaa cgatcaacaa ggctggtatg ccgattacga cctgaaaagt cataaagtgc
    21361 gcaatcagtt aaccgcggcc gccctgttcc cgctgtacgt caatgcggca gcgaaagatc
    21421 gcgccagcaa aatggcgacg gcgacgaaaa cacatctgct gcaaccaggc ggcctgaaca
    21481 ccacgtcggt gaaaagtggg caacaatggg atgcgccaaa cggctgggca ccgttgcagt
    21541 gggtcgcgac agaaggatta caaaactacg ggcaaaaaga ggtggcgatg gacattagct
    21601 ggcacttcct gaccaatgtt cagcacacct atgaccggga gaaaaagctg gtggaaaaat
    21661 atgatgtcag caccaccgga acagggggcg gcggtggcga atatccatta caggatggct
    21721 ttggctggac caatggcgtg acgctgaaaa tgctggattt gatctgcccg aaagagcaac
    21781 cgtgcgacaa tgttccggcg acgcgtccac tcagtgaatc aacaacacag ccggtaaaac
    21841 aaaatgaggc ggaacctacg ccttaacttt actgtatatc gagcaatgcc attacgtgca
    21901 taaaattggc
//
LOCUS       DAZUCC010000068        21888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZUCC010000068 DAZUCC010000000
VERSION     DAZUCC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.068"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..2129)
                     /gene="glyS"
                     /locus_tag="ACHY09_002225"
     CDS             complement(60..2129)
                     /gene="glyS"
                     /locus_tag="ACHY09_002225"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS3885194.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNTNVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2139..3050)
                     /gene="glyQ"
                     /locus_tag="ACHY09_002226"
     CDS             complement(2139..3050)
                     /gene="glyQ"
                     /locus_tag="ACHY09_002226"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS3885195.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3145..3453)
                     /locus_tag="ACHY09_002227"
     CDS             complement(3145..3453)
                     /locus_tag="ACHY09_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFS3885196.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            3627..4622
                     /gene="wecH"
                     /locus_tag="ACHY09_002228"
     CDS             3627..4622
                     /gene="wecH"
                     /locus_tag="ACHY09_002228"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFS3885197.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVCAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4664..5101)
                     /gene="yiaA"
                     /locus_tag="ACHY09_002229"
     CDS             complement(4664..5101)
                     /gene="yiaA"
                     /locus_tag="ACHY09_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFS3885198.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5146..5487)
                     /gene="yiaB"
                     /locus_tag="ACHY09_002230"
     CDS             complement(5146..5487)
                     /gene="yiaB"
                     /locus_tag="ACHY09_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFS3885199.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGILMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            complement(5656..7110)
                     /gene="xylB"
                     /locus_tag="ACHY09_002231"
     CDS             complement(5656..7110)
                     /gene="xylB"
                     /locus_tag="ACHY09_002231"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS3885200.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWSMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7182..8504)
                     /gene="xylA"
                     /locus_tag="ACHY09_002232"
     CDS             complement(7182..8504)
                     /gene="xylA"
                     /locus_tag="ACHY09_002232"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFS3885201.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8870..9862
                     /gene="xylF"
                     /locus_tag="ACHY09_002233"
     CDS             8870..9862
                     /gene="xylF"
                     /locus_tag="ACHY09_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3885202.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9940..11481
                     /gene="xylG"
                     /locus_tag="ACHY09_002234"
     CDS             9940..11481
                     /gene="xylG"
                     /locus_tag="ACHY09_002234"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFS3885203.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11459..12640
                     /gene="xylH"
                     /locus_tag="ACHY09_002235"
     CDS             11459..12640
                     /gene="xylH"
                     /locus_tag="ACHY09_002235"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFS3885204.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12718..13896
                     /gene="xylR"
                     /locus_tag="ACHY09_002236"
     CDS             12718..13896
                     /gene="xylR"
                     /locus_tag="ACHY09_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFS3885205.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14004..14828)
                     /locus_tag="ACHY09_002237"
     CDS             complement(14004..14828)
                     /locus_tag="ACHY09_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFS3885206.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15148..17175
                     /gene="malS"
                     /locus_tag="ACHY09_002238"
     CDS             15148..17175
                     /gene="malS"
                     /locus_tag="ACHY09_002238"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFS3885207.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALVEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAA
                     SVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17353..18606
                     /gene="avtA"
                     /locus_tag="ACHY09_002239"
     CDS             17353..18606
                     /gene="avtA"
                     /locus_tag="ACHY09_002239"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFS3885208.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRIKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMSEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18758..19231)
                     /locus_tag="ACHY09_002240"
     CDS             complement(18758..19231)
                     /locus_tag="ACHY09_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS3885209.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19333..20181)
                     /gene="yiaJ"
                     /locus_tag="ACHY09_002241"
     CDS             complement(19333..20181)
                     /gene="yiaJ"
                     /locus_tag="ACHY09_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFS3885210.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20382..21380
                     /gene="yiaK"
                     /locus_tag="ACHY09_002242"
     CDS             20382..21380
                     /gene="yiaK"
                     /locus_tag="ACHY09_002242"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFS3885211.1"
                     /translation="MKVTFEQLKAAFNRVLISRDVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21392..21859
                     /locus_tag="ACHY09_002243"
     CDS             21392..21859
                     /locus_tag="ACHY09_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFS3885212.1"
                     /translation="MIFGHIAQPDPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAAENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTTSEIRKIVVKVALTALN"
BASE COUNT         5529 a         5575 c         5363 g         5421 t
ORIGIN      
        1 ctctattatt tagcgataaa aaagcctgcc agatggcagg ctttttaata acggcgttat
       61 tactgtaaca gcgaaatatc cgcaacgcgc aggaataatt cgcgcagttt ctccagcatg
      121 gtaagacggt tgattcgcaa ttctttgtca tcaaccatga ccatcacttt atcgaagaag
      181 gcatcaaccg gttcacgcaa ttcagccagt tcgaccagcg cgtcctggta acgaccttca
      241 gcgaagtacg gctccagctt gtcgcgcagc accacaactt gcatcgccag tttaatttct
      301 tccggctctt tcagagtaga agcattcacg cgatcgctca gcacttcgtc agacttcgcc
      361 agaatgttag atacacgctt gtttgccgcc gccagtgacg ctgccgcatc cagagtacgg
      421 aagtgcgata ccgccttcat acgggcatcg aaatctgccg gacgagtcgg acgacgcgcc
      481 agtaccgcct ggatggtatc aacggtgtaa ccttcgtcct gataccaggc gcggaagcga
      541 ccgagcataa agtcgataac atcatcaact acgttggtat tagtcagctt atcgccatac
      601 agacgcaccg cttcttcggt cagcgtttgc agatcaaggt tgaggttctt ctcaacgata
      661 atacgcagca cgccaagtgc ggcacgacgc aacgcaaacg ggtctttgtc gcctttcgga
      721 tgctggccga taccgaagat acccgccaga gtatccatct tgtcagcaat cgccagcgca
      781 caggctaccg ggttagacgg caggtcatca ccggcaaagc gcggctgata ctgctcgttc
      841 agcgccacgg caacatcttc cgcttcgcca tcgtgacgcg cgtagtgcat ccccataacg
      901 ccctgggtgt cggtgaactc gaagaccatg ttggtcatca ggtcgcactt ggacagcagg
      961 cccgcacggg tagcgtggtt aacgtcagcg ccaatctgtt cagcaatcca gccagccagc
     1021 gcctggatgc ggtcagtctt gtcacgcagc gtacccagct gttgctggaa cagcacggtt
     1081 tgcaggcgcg gcaggttatc ttccagacgt tttttacggt cggtgttgaa gaagaactcg
     1141 gcatccgcca gacgtggacg aacgactttc tcgttaccag agataatttg ctgcggatct
     1201 ttcgattcga tgttggcaac gaagataaag ttcggcagca gtttgccgtc gttcgcatac
     1261 accgggaagt atttctggtc acctttcatg gtgtaaacca gcgcttcaga cggcaccgcg
     1321 aggaatttct cttcgaattt tgcggtcagc acaaccggcc attccaccag cgaagcgact
     1381 tcttccaaca ggctttcgct taagtcagcg ttaccgccaa tcttacgcgc cgcttcttcg
     1441 gcatcggctt taatcttcgc cttacgttct tcgtaatcgg cgatgacttt cccgcgctca
     1501 cgcagaattt ccggatactg atcagcattg tcgatagtga attccggctc gcccataaag
     1561 cggtggccgc gaatcacgcg atcggactga atgcccagaa tggtagccgg aataactttg
     1621 tcgcccagca gcagggtcac ggtgtgcacc ggacgcacga agtgcacgtc gcttgcaccc
     1681 caacgcatca gtttcgggat cggcagtttc gccagcgaag tcgcaaccat attcggcagc
     1741 agtgcttcag tgctttcgcc cttcacgtgg gcgcgataca gcagccattc gcctttatcg
     1801 gtagtcagac gctcagcctg gtcaacggta ataccgcaac cacgcgccca gccttctgcc
     1861 gctttgctcg gtttgccttc agcgtcgaac gcctgggcaa tcgccgggcc gcgtttttcg
     1921 atttcacgat ccggttgcgc ttccgccagg ttagctactt taagcgccag acgacgcgga
     1981 gcagcaaacc attgaacggt gccgtgtgcg aggccagcgt tatccagctc cgcagtaaag
     2041 ttcgcagcaa aggactcagc caggctgcgc agtgcttttg gtggcagctc ttcagtgccg
     2101 atttccacca gaaaagtttt ctcagacata gccgcctctt acttatcttt gttgcacatc
     2161 gggaagccga gggcttcacg ggaagcgtag tatgcttctg ccactgcttt ggtcagggtg
     2221 cgaatgcgca gaatatagcg ctgacgctcg gtgacggaga tggctttacg cgcatccagc
     2281 aggttgaagc tgtgggcggc tttcagaata cgctcgtagg ctggcagcgg cagcggattt
     2341 tccagcgcca gcagctgctg cgcttctttc tcgtactgct caaagcaggt gaacaggaag
     2401 tccacatccg cgtattcgaa gttgtaagtg gactgctcca cttcgttctg atggaacacg
     2461 tcgccatagg tggttttacc cagcgggccg tcgctccaga ccaggtcgta aacgctgtct
     2521 acgccctgaa tgtacattgc cagacgttcc agaccgtagg tgatctcgcc ggtaaccggt
     2581 ttacactcca gaccaccaac ctgctggaag taagtgaact gcgtcacttc catgccgttc
     2641 agccacactt cccagcccag cccccaggca cccagcgtcg ggttttccca gttatcttcc
     2701 acgaaacgga tgtcgtggat ggtcgggtcc atgcccagct ctttcagaga accaaggtac
     2761 agctcctgaa tattgtccgg cgatggctta atgaccacct ggaactgata gtagtgctgt
     2821 aaacggttgg ggttttcgcc gtagcgacca tcggtcgggc gacgagaagg ctgaacataa
     2881 gcagccgcca tcggttctgg ccccagcgcg cgcagacagg tcattgggtg agaggttccc
     2941 gcgccgactt ccatgtccaa tggttgaaca atggtgcagc cctggcgagc ccagtaatcc
     3001 tgtaaggtca ggatcaagcc ctggaaggtc ctggtatcaa acttttgcat attatttcgt
     3061 gctggatacg tgtggattta aaggaaggga tcagtatacc cgctggatgg aagatataca
     3121 gtacgaaacg ggaaaaagca gggcttaacg catggaaaga tgcaaaaaat ggccatctgc
     3181 gtcaaaagaa cagacaaact gctctttacg gaaggtataa ccgcgcattt cgtagcttcc
     3241 ctggaactgt tcaaatgcgg tgacgtcgat tttttgctcg ccggtgttat aacgtttcgc
     3301 cgcctgatcc ttgcataacg cttccatatt caacgaacgc agaggatcaa cctttacccg
     3361 ttgtgctttc tgcacgggtg gcggtgtaga acaacccact agcgccataa gcaccataag
     3421 cgccattgcc ggaaagaaag agttcatcat cattaaatta ccgtctggtt tgcaggtgag
     3481 tcttattatt tatatcggta tagtggctga tatcttacga atttccttaa gcctctataa
     3541 gctgtagtga taaaactcag attattccag tgcgccaacc gaatgattta tcggtcagac
     3601 ttttttaacc agatacagag acacagatgc agcccaaaat ttactggatt gataacctgc
     3661 gagggatagc gtgtttaatg gtggtgatga ttcacaccac tacctggtat gtgaccaatg
     3721 ctcatagtgt tagccccgtc acctgggata tcgccaatgt tctgaattct gcctctcgtg
     3781 tcagcgtgcc gctatttttc atgatttccg gctatctctt ttttggcgaa cgcagcgccc
     3841 agccgcgcca tttcttgcgt atcggcttat gtctgctttt ttatagcgca atcgcactgc
     3901 tctacattgc gctgtttacc tccatcaatg tggagttagc gctgaaaaac ctgctgcaaa
     3961 agccagtgtt ttaccactta tggtttttct tcgcgattgc ggtgatttat ctggtttcac
     4021 cgctgattca ggtgaagaac gtcggcggaa aaatgttgct ggtgctaatg gtggtgattg
     4081 gtattatcgc taacccaaac acagtgccgc agaaaattga cggttttgaa tggctgccaa
     4141 ttaacttata tatcaatggc gatacttttt actacatcct gtatggcatg ttgggccgcg
     4201 ctatagggat gatggacaca cagcataaag cactgtcgtg ggtgtgcgca gcactgtttg
     4261 cgacgggggt ttttattatc tctcgcggga cattatatga attgcagtgg cgcggaaatt
     4321 ttgccgatac ctggtatctt tactgtgggc cgatggtttt tatctgcgca atcacgctat
     4381 tgactctggt taaaaacacg cttgatacgc gtaccgttcg cggacttggc ttaatctccc
     4441 gccattcgtt gggcatatac gggttccacg ccttgattat ccatgcactg cgcacccggg
     4501 ggattgagct taaaaactgg ccaatactcg atattatttg gatcttttgc gcgacgttgg
     4561 cagcgagttt gttactttct atgctggtac aacgaatcga cagaaacaga ctagtgagtt
     4621 aagtaaaagg ccggtcacat tggactgacc gggcttatgt gaattattcg ctgtattctt
     4681 cctgggtaac ctgtgtttct tttggtgggt ttattccggc atcacgaata ttcttctgca
     4741 ccgctactgc accaaacaag cttaagaaga aagccagtcc ataaaaacct ttctcgctga
     4801 gtaataacgt tgcattccac agacctacca tcagtaacgc aacagagata ataaagacag
     4861 tcaggcaggt catataataa atggaggtgg tcggtatgcc ttcatacttg tcccgaacgg
     4921 tcttttgata agacgccgca gaaaacagtc ctaataccag tacggcaaaa taatatcctt
     4981 tttcatttaa ctgcatctct gcattccata gccctaacag ataggtaacg ataccaccaa
     5041 cgagagctat ccatgacaca atactgaatg ccggagaata ggttgatatt ttgttgtcca
     5101 tcacagtatt ccttcattcc tgaatgtgta agagctttat gttgctcatg ccgagcgaaa
     5161 caaacgcatt tgaccaaaca agcagacaaa aaaggcgata gggtagatca ccatttcata
     5221 agcattgata ggtgcgttta aaacaccgat aaacaagaga ccgatagtga ttaacgcaac
     5281 taactggcag atatgggtaa aaaaattatc cagttgcccg agtttttctg cgcgaagata
     5341 tgcatagttg ccaaaagttg ctgtcattaa tatgccaaga aaatagcctt ttccacttaa
     5401 caatgggcat gagatccata gcccaaccag ataaaccagc gccccgacaa caaataatag
     5461 ttttgccaca gtctttgagg ttttcataac gatctccata tctaccagcg atacattacg
     5521 agtaaccaac gaaagacaaa actgaaaaat gccattaaca aatgattttc agaataaatt
     5581 catactaaat attaattaat tactgagata tatagatgtg aattatcccc cacccggtca
     5641 ggcagaggat aacgtttacg ccattaatgg cagaagttgc tgatagaggc gacggaacgt
     5701 ttctcgtcgt ggctgataag cggcataacg ctgcgcatct ggtagatgcg actgttctaa
     5761 cggtaactgc ggcaacaact caatgagcga tttctctgga ttcgccgcaa tctgcgccag
     5821 ccttgctgcg cccagtgctg gccccacatc cccccccgta cggtaatcga gctgctgacc
     5881 gctgatatcc gccagcattt gacgccagta ctcactacgc gccccgccac caatcaacgt
     5941 aacactttgc ggtttaatgc cgcaggcatg cacgacatcc atgccatctg ccagcgcata
     6001 acccacgcct tccagcactg ctcgcgccag ttcattgggg ccatgttgat gagtcaaacc
     6061 aaagaaaacc cccttcgcct ggggattatt gtgtggcgta cgctcgccgg aaagataagg
     6121 cagaaaccaa actggctcgg cactttcatc agcctgttga gctgcagcga ttaaagctgg
     6181 gacattgctc aggccggtta atttcgcggc ccaatccaga cacgacgctg cactcagcat
     6241 tacagacatt aaatgccaac gctgcggtag cgcatggcaa aagctgtgta cggcgctttc
     6301 tggcttgctt aaaaaccctt cgctgacagc aaaatagacc cccgacgtcc ccagcgataa
     6361 cattgcctga ttagcatcaa ccattcctac accaaccgca ccagctgcat tatcaccacc
     6421 gcctgcgaca actggcaccg ccgccatact ccacgctttc gcaacttcag gtagcaaagc
     6481 accagtaatt tcgctgcctt cgtataatgc gggcatctgg tcacgagata agtggcaagc
     6541 ctgcagcatg tcgtcactcc agtcacgctt tgcgacatcc agccacatgg tgccagctgc
     6601 gtcagacata tcgctggcaa actcccccgt catacgcaga cgcaagtaat ctttcggtaa
     6661 taatactttg tcgatttgac ggaatatctc cggctcatgc cgctgaaccc atagcaattt
     6721 aggcgcagta aatccgggca tcatcaggtt accggtaatc acccgtgatt gcggaactcg
     6781 cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg ttccacaaaa tggcagggcg
     6841 taatacccgt tgctgggcat ccagtaaggt tgcaccgtgc atctggccgg caatacccaa
     6901 tgctttaacg tcctgcagag aatgctgatc gcccagagct ttcattgcgc ggtcagttgc
     6961 ctgccaccac tgttccgggt cttgttccga ccagagtgga tgcgggcgcg aaacggtcag
     7021 cttttccgtt tgcgaagcaa ccacctcacc ctgctcgttg agcaaaataa cttttacgcc
     7081 cgaggtgcca agatctatcc cgatatacat atcgatcgtt ccttaaaaaa atgcccggta
     7141 tcgctaccga taaccgggcc aacggactgc acagttagcc gttatttgtc gaacagataa
     7201 tgatttacca gattttccag ttgctcctgg cgaccactct gatgcaccgg agacaaattg
     7261 tgttcctgag catatttggc taaatctgcc agtgacattt ggcctttcag gatttgctgg
     7321 cctaattcgc tattccagcc ggaataacgc tgcgcgatgc gtttatccag ctcgccatct
     7381 tcaatcatgc gtgctgcaat tttcagcgcc aacgccatcg tatccatcgc gccgatatga
     7441 ccgtaaaaca gatcatattt atcagtactt tgacgacgta ctttggcatc gaagttcaga
     7501 ccgccagtgg tgaaaccgcc tgctttgaga atttcataca tcaccagcgc attctcttcc
     7561 acactgttcg ggaactggtc ggtgtcccag cccagttgtg catcgccacg gttggcgtcg
     7621 acagaaccga acaggccaag cgcaatggcg gtggctattt catgatggaa agagtgacct
     7681 gccagcgtcg cgtggttagc ttcaatattc agtttaatct ctttttccag accaaactgt
     7741 ttcaggaagc catagaccgt cgcagcatcg taatcatatt gatgtttggt cggttcttgc
     7801 ggtttcggtt cgataagcaa cgtgccctgg aaaccgattt tatgtttatg ctcaaccacc
     7861 atctgcataa agcggcccag ctgttcacgc tcctggcgca agtcggtatt taacagcgtt
     7921 tcgtaacctt cacgaccacc ccacaggaca tagttttcac cgcccaattt atgggttgct
     7981 tccatcgctg taacaacttg cgttgccgcc caactgaaga cttcaggatc tgggttcgtc
     8041 gccgcacccg cgccatagcg agggtttgta aagcagttag cagttcccca cagcagcttc
     8101 acgccgctct cttcttgctt gcctgccagg acatcaacca tttgcgcaaa attattgatg
     8161 tactctttta acgacgcgcc ctcaggggaa acatccacat cgtggaagca ataaaatggc
     8221 acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt tacgcttcgc caacgccagt
     8281 gcctcaccag gctgctgcca cggacgatta aacgctccca caccaaacat atccgccccg
     8341 ttccagcaga aggtgtgcca gtagcaggcg gcaaaacgca agtgctcttc catacgcttg
     8401 cccaacacca gttcgtcggg attgtagtga cggaatgcta acgggtttga ggattttgag
     8461 ccttcataac gaacgcgatc gagctggtca aaataggctt gcatattgaa ctccataatc
     8521 aggtaatgcc gcgggtgatg gatgatgtcg taatattggg cactcccttt cagttgctca
     8581 attatgttat ttcacactgc tattgagata attcacaagt gtgcgctcgc tcgcaaaata
     8641 aaatgggatg atgaaactgg gtaattcctc gaagagaaaa atgcaataag tacaattgcg
     8701 caacaaaagt aagatctcgg tcataaatca agaaataaac caaaaatcgt aatcgaaaga
     8761 taaaaatctg taattgtttt cccctgttta gttgctaaaa attggttacg tttatcgcgg
     8821 tgattgttac ttattaaagc tgtcctctaa ctacagaagg ccctacacca tgaaaataaa
     8881 gaacattcta ctcacccttt gcacctcact tctgcttacc aacgttgcgg cacacgccaa
     8941 agaagtcaaa ataggtatgg cgattgatga tctccgtctt gaacgctggc aaaaagatcg
     9001 agatattttt gtgaaaaaag cagaatctct cggcgcgaaa gtatttgtac agtctgcaaa
     9061 tggcaatgaa gaaacacaaa tgtcgcagat tgaaaacatg attaaccggg gtgtcgatgt
     9121 tcttgtcatt attccttata acggtcaggt attaagtaac gttgtaaaag aagccaaaca
     9181 agaaggtatt aaagtattag cttacgaccg tatgattaac gatgcggata tcgattttta
     9241 tatttctttc gataacgaaa aagtcggtga actgcaggca aaagccctgg tcgatattgt
     9301 tccgcaaggt aattacttcc tgatgggcgg ctcgccggta gataacaacg ctaagctgtt
     9361 ccgcgccgga caaatgaaag tgttaaaacc ttacgttgat tctggaaaaa ttaaagtcgt
     9421 tggtgaccaa tgggttgatg gctggttacc ggaaaacgca ttgaaaatta tggaaaacgc
     9481 gctaaccgcc aataataaca aaattgatgc tgtagttgcc tcaaacgatg ccaccgcagg
     9541 tggggcaatt caggcattaa gcgcgcaagg tttatcaggg aaagtagcaa tttccggcca
     9601 ggatgcggat ctcgcaggta ttaaacgtat tgctgccggt acgcaaacta tgacggtgta
     9661 taaacctatt acgttgttgg caaatactgc cgcagaaatt gccgttgagt tgggcaatgg
     9721 tcaggaacca aaagcagata ccacactgaa taatggcctg aaagatgttc cctcccgcct
     9781 gctgacaccg atcgatgtga ataaaaacaa catcaaagac acggtagtta aagacggatt
     9841 ccacaaagag agcgagctgt aagcgttacg ccccggcgag gagcgggggc gtgatttctc
     9901 tccatgccgt gtgaattaat tggcttaggt ggagtcgtta tgccttatct acttgaaatg
     9961 aagaacatta ccaaaacctt cggcagtgtg aaggcgattg ataacgtcag cttgcggttg
    10021 aatgctggcg aaatcgtctc actctgtggg gaaaatgggt ctggtaaatc aacgctgatg
    10081 aaagtgctgt gtggtattta tccccatggt tcctacgaag gcgaaattat ttttgcggga
    10141 gaagagattc aggcgagtca catccgcgat accgaacgca aaggtatcgc catcattcac
    10201 caggaattgg ccctggtgaa agaattgacc gtgctggaaa atatcttcct gggtaacgaa
    10261 ataacccata atggcattat ggattatgat ctgatgacgc tacgctgtca gaagctgctc
    10321 gcacaggtca gtttatccat ttcacctgat acccgcgttg gcgatttagg acttgggcaa
    10381 caacaactgg ttgaaattgc caaggcactt aataaacagg tgcgtttgtt aattctcgat
    10441 gagccgacag cctcattaac tgagcaggaa acgtcggttt tactggatat tattcgcgat
    10501 ctacaacagc acggtatcgc ctgtatttat atttcgcaca aactcaacga agtcaaagcg
    10561 atttccgata cgatttgcgt tattcgcgac ggtcagcaca ttggcacgcg tgatgctgct
    10621 ggaatgagtg aagacgatat tatcaccatg atggtcggac gagagttaac cgcgctttac
    10681 cctaatgaac cacataccac cggagatgaa atattacgta ttgaacatct gacggcatgg
    10741 catccggtta atcgtcatat taaacgagtt aatgatgtct cgttttccct gaaacgtggc
    10801 gaaatactgg gtatcgccgg actcgttggt gccggacgta ccgagactat tcagtgcctg
    10861 tttggtgtgt ggcccggaca atgggaaggg aaaatttata ttgatggcaa acaggtagat
    10921 attcgtaact gtcagcaagc catcgcccag gggattgcga tggtacccga agacagaaag
    10981 cgcgacggca tcgttccggt aatggcggtt ggtaaaaata ttaccctcgc cgcactcaaa
    11041 aaatttaccg gtggcattag ccagcttgat gacgcggcag agcaaaaatg tattctggaa
    11101 tcaatccagc aactcaaagt taaaacgtcg tcccccgacc ttgctattgg acgtttgagc
    11161 ggcggcaatc agcaaaaagc gatcctcgct cgctgtctgt tacttaaccc gcgcattctc
    11221 attcttgatg aacccaccag gggtatcgat attggcgcga aatacgagat ctacaaatta
    11281 attaaccaac tcgtccagca gggtattgcc gttattgtca tctcttccga attacctgaa
    11341 gtgctcggcc ttagcgatcg tgtactggtg atgcatgaag ggaaactaaa agccaacctg
    11401 ataaatcata acctgactca ggagcaggtg atggaagccg cattgaggag cgaacatcat
    11461 gtcgaaaagc aatccgtctg aagtgaaatt agccgtaccg acatccggtg gcttttccgg
    11521 gctgaaatca ctgaatttgc aggtcttcgt gatgattgca gctatcatcg caatcatgct
    11581 gttctttacc tggaccaccg atggtgccta cttaagcgcc cgtaacgtct ccaacctgtt
    11641 acgccaaacc gcgattaccg gcatcctcgc ggtaggaatg gtgttcgtca taatttctgc
    11701 tgaaatcgac ctttccgtcg gctcaatgat ggggctgtta ggtggcgtcg cggcgatttg
    11761 tgacgtctgg ctaggctggc ctttgccact taccatcatt gtaacgctgg ttctgggatt
    11821 gcttctcggt gcctggaacg gatggtgggt cgcgtaccgc aaagtccctt catttattgt
    11881 caccctcgcg ggcatgttgg catttcgcgg catactcatt ggcatcacca acggcacgac
    11941 cgtatccccc accagcgccg cgatgtcaca aattgggcaa agctatctac ctgccagcac
    12001 tggcttcatc attggcgcgc ttggcttaat ggcttttgtt ggctggcaat ggcgcggaag
    12061 aatgcgccgt caggctttgg gtttgcaatc tcccgcctct accgcagtag ttggtcgcca
    12121 ggctttaacc gctatcatcg tattaggcgc aatctggctg ttgaatgatt accgtggcgt
    12181 tcccactcct gttctgctgc tgacgttgct gttactcggc ggaatgttta tggcaacgcg
    12241 gacagcattt ggacgacgca tttatgccat cggcggcaat ctggaagcgg cacgtctctc
    12301 cgggattaac gttgaacgca ccaaacttgc cgtgttcgcg attaacggat taatggtagc
    12361 catcgccgga ttaatcctca gttctcgact tggcgctggt tcaccttctg cggggaatat
    12421 cgccgagctg gacgcaattg cagcatgtgt gattggcggc accagtctgg caggcggcgt
    12481 cgggagcgtg gctggagcgg taatgggggc attcatcatg gcttcactgg ataacggcat
    12541 gagtatgatg gatgtaccga ccttctggca gtatatcgtt aaaggtgcga ttctgttgct
    12601 ggctgtatgg atggactccg caaccaaacg ccgttcttga ttttgataaa aattttctca
    12661 aagccggtaa cgtattaccg gttttgagtt tttgcatgat tcagcaggaa aagaaccatg
    12721 tttactaaac gtcaccgcat cacattactg ttcaatgcca ataaagccta tgaccggcag
    12781 gtagtagaag gcgtagggga atatttacag gcgtcacaat cggaatggga tattttcatt
    12841 gaagaagatt tccgcgcccg cattgataaa atcaaggact ggttaggaga tggcgtcatt
    12901 gccgacttcg acgacaaaca aatcgagcaa gcgctggctg atgtcgacgt ccccattgtt
    12961 ggggttggtg gctcgtatca ccttgccgaa agttacccac ccgttcatta cattgccacc
    13021 gataactatg cgctggttga aagcgcattt ttgcatttaa aagagaaagg cgttaaccgc
    13081 tttgcttttt atggtcttcc ggaatcaagc ggtaaacgtt gggccactga acgcgaatat
    13141 gcatttcgtc agcttgtcgc cgaagaaaag tatcgcggag tggtttatca ggggttagaa
    13201 accgcgccag agaactggca acacgcgcaa aatcggctgg cagactggct acaaacgctg
    13261 ccaccgcaaa ccgggattat tgccgttact gacgcccggg cgcggcatat tctgcaagta
    13321 tgtgaacatc tacatattcc cgtaccggaa aaattatgcg tgattggcat cgataacgaa
    13381 gaactgaccc gctatctgtc gcgtgtcgcc ctttcttcgg tcgctcaggg cgcgcggcaa
    13441 atgggctatc aggcggcaaa actgttgcat cgattattag ataaagaaga aatgccgcta
    13501 cagcggattt tggtcccgcc agttcgcgtc attgaacggc gctcaacaga ttaccgctcg
    13561 ctgaccgatc ccgccgttat tcaggccatg cattacattc gtaatcacgc ctgtaaaggg
    13621 attaaagtgg atcaggtact ggatgcggtc gggatctcgc gctccaatct tgagaagcgt
    13681 tttaaagaag aggtgggtga aaccatccat gccatgattc atgccgagaa gctggagaaa
    13741 gcgcgcagtc tgctgatttc aaccaccttg tcgatcaatg agatatcgca aatgtgcggt
    13801 tatccatcgc tgcaatattt ctactctgtt tttaaaaaag catatgacac gacgccaaaa
    13861 gagtatcgcg atgtaaatag cgaggtcatg ttgtagcgat gatgagaatt gtcggcgtcg
    13921 catcaggtaa ctcagcacaa ccacctgatg caagcaaaag ccttatccga ctgacaagtc
    13981 ggataaggct cgaggaaatg caattacata tgcgccgcga ttaaccgttg gttatcctgg
    14041 tacattgcga acaggtagtt gttataactc ttccctttag tcgagtagcc cttcagcttg
    14101 tgaatcatcg ctgtggcagt cacttcctga tccgctttac gcagctgtgc acgcgattta
    14161 cggaacgaag agtaagccgg atgagtattc aggttagtga cataggcgct caccgattct
    14221 ttgacagaac taaactgtga gtaccctttc actttacccg gcgcattggt acaacgtcct
    14281 ttcatgcatt tcatgccgaa cagattgttg ttgttgcgcg ccagctttga cgttccccaa
    14341 ccgctttctg ctgcagccat cgtcgccacc atactggtag ggataatgtc tacgcgttca
    14401 agcaaggtat tccacgggat ttttcgcgta ttaccagacc acttcacctt gtagcgtttg
    14461 gcgatgtctt tcagacgcgc acgctcagca ggtgaccatt ggccctgata ctgttttgaa
    14521 atcagccagt tacgttccgc agtaatggcc gcattttggc tggtaatgta aggcattaca
    14581 gtccggagaa acgccttttt ccttggtgtt ccggaagggt attttcgcaa atcaggaagt
    14641 gaactactct ttgcactatt gcgagaatac tcttgtttac tgcttacctg tttattacta
    14701 gctttagtta agtgggactt ttgactcgct gttgttgtgt gcgtcttcgc taacacctca
    14761 ctcgaaaaca ccagagtgag taacataaga atcatcgccc catatcgtcg tatgggagtc
    14821 aaaatcatca ggtctcctgg tcggatttaa tcattccaac accttatatt tttcacaaat
    14881 ttgagagttg aatctcaaat catatcaaaa atagctgtca agagcacccc aaggaatagt
    14941 ccaaatctga aactatgtca cgagttaacg attcagattg gcgctaaatc gcagaaaatg
    15001 tggggtttat cgcaaaattc agccgttttt tgtgcgcgat cgctcaccct tgcttctcat
    15061 cctgtggact taccgctcag ggatgagttt tgtctggctt atcgctggca aactgtctga
    15121 aatcgcagca ataaggactc atccgccatg aaactcgccg cctgttttct gacactcctt
    15181 cctggcttcg ccgttgccgc cagctggact tctccggggt ttcccacctt tagcgaacag
    15241 gggacgggaa catttgtcag ccacgcgcag ttgcccaaag gtacgcgtcc actaacgcta
    15301 aattttgacc aacagtgctg gcagcctgcg gatgcgataa aactcaatca gatgctttcc
    15361 ctgcaacctt gtagcaacac gccgcctcaa tggcgattgt tcagggacgg cgaatatacg
    15421 ctgcaactag acacccgctc cggtacacca acattgatga tttccatcca gaacgccgtc
    15481 gaaccggtag caagcctggt ccgtgaatgc ccgaaatggg atggattacc gctcacactg
    15541 gatgtcagcg ccacttttcc ggagggagcc gccgtccggg attattacag ccagcaaatt
    15601 gcgatagtga agaacggtca aataacgtta caacccgctg ccaccagcaa cggtttactc
    15661 ctgctggaac gggcagaaac tgacgcccct gcccctttcg actggcataa cgccacggtt
    15721 tactttgtgc tgacagatcg tttcgaaaac ggcgatccca gtaatgacca gagttacgga
    15781 cgtcataaag acggtatggc agaaattggc acttttcacg gcggcgattt acgcggcctg
    15841 accaacaaac tggattacct ccagcagctg ggcgtcaatg ctttatggat aagtgcccca
    15901 tttgagcaaa ttcacggctg ggtcggcggc ggtacaaaag gcgatttccc gcattatgcc
    15961 taccacggtt attacacaca ggactggacg aatcttgatg ccaatatggg cagtgaagcc
    16021 gatctacgga cactggttga tagcgcgcat cagcgcggta ttcgtattct ctttgatgtc
    16081 gtgatgaacc acaccggcta tgccacactg gcggatatgc aggagtatca gtttggcgcg
    16141 ttatatcttt ctggtgacga agtgaaaaaa acgttgggtg aacgctggag cgactggaaa
    16201 cctgccgccg ggcaaacctg gcatagcttt aacgattaca ttaatttcag cgacaaaaca
    16261 ggctgggata aatggtgggg aaaaaactgg atcagaaccg atatcggcga ttacgacaat
    16321 cctggatttg acgatctcac tatgtcgctg gcctttttgc cggatatcaa aaccgaatca
    16381 acgaccgctt ctggtctgcc ggtgttctat aaaaacaaaa cggataccca cgctaaagtc
    16441 atcgacggct ttacccctcg cgattactta acccactggt taagtcagtg ggttcgcgac
    16501 tatgggattg atggttttcg ggtcgacacc gccaaacatg ttgagttgcc cgcctggcag
    16561 caactgaaaa ccgaagccag cgccgcgctt gtcgaatgga aaaaagcgaa tcccgataaa
    16621 gcgttagatg acaaaccgtt ctggatgacc ggtgaagcct ggggccacgg cgtgatgcaa
    16681 agtgactact atcgccacgg cttcgatgcg atgatcaatt tcgattatca ggagcaggcg
    16741 gctaaagcag tcgattgtct ggcgcagatg gatacgacct ggcagcaaat ggcggagaaa
    16801 ttgcaggatt tcaacgtgtt gagctacctc tcgtcgcatg atacccgcct gttccgtgaa
    16861 gggggcgaca aagcagcaga gttattatta gcgccaggcg cggtacaaat cttttatggt
    16921 gatgaatcct cgcgtccgtt cggccccacc ggttcggatc cactgcaagg cacgcgttcg
    16981 gatatgaact ggcaggatgt tagcggcaaa tctgccgcca gcgtcgcgca ctggcagaaa
    17041 atcagccagt tccgcgcccg ccatcccgca attggcgcgg gcaaacaaac gacactttcg
    17101 ctgaagcagg gctacggctt tgttcgtgag catggcgacg ataaagtgct ggtcatctgg
    17161 gctgggcaac agtaactttt ccggcttccc gttcgtcagt acctcgggaa gccgccaacc
    17221 aggataaaat gtcagaccta atcagcgttg caggataaag caccgctccc tcttcaacag
    17281 agcgatttgc accccagcaa atgtagcgtt attgttacct tccttgctac agagttcgac
    17341 agatatcccg ctatgacatt ctcccttttt ggtgacaaat ttacccgcca ctccggcatt
    17401 acgctgctga tggaagatct gaacgacggt ttacgcacgc ctggcgcgat tatgctcggc
    17461 ggcggtaatc cagcgcagat cccggaaatg caggactact tccagacgct actgaccgac
    17521 atgctggaaa gtggcaaagc gactgatgca ctgtgcaatt acgacggtcc acaggggaaa
    17581 acggagctac tcacactgct tgccggaatg ctacgcgaga agttgggttg ggatatcgaa
    17641 ccacagaata ttgcactaac aaacggcagc cagagcgcgt ttttctactt atttaatctg
    17701 ttcgccggac gccgtgccga tggtcggatc aaaaaagtgc tgtttccgct ggcaccggaa
    17761 tacattggct acgctgacgc cggactggaa gaagatctgt ttgtctctgc gcgtccgaat
    17821 attgagctgc tgccggaagg ccagtttaaa taccacgtcg attttgagca tctgcatatt
    17881 ggcgaagaaa ccgggatgat ttgcgtctcc cggccgacga acccaacagg caatgtgatt
    17941 actgacgaag agttgctgaa gcttgacgcg ctggcgaatc aacacggcat tccgctggtg
    18001 attgataacg cttatggcgt cccgttcccg ggtatcatct tcagcgaagc gcgcccgctg
    18061 tggaatccga atatcgtact gtgcatgagt ctttccaagc tgggtctacc aggctcccgc
    18121 tgcggcatta tcatcgccaa tgaaaaaatc atcaccgcca tcaccaatat gaacggcatt
    18181 atcagcctgg cacctggcgg tattggtccg gcgatgatga gtgaaatgat taagcgtaac
    18241 gatctgctgc gcctgtctga aacagtcatc aaaccgtttt actaccagcg tgttcaggaa
    18301 actatcgcta tcattcgccg ctatttaccg gaagatcgct gcctgattca taaaccggaa
    18361 ggagccattt tcctctggct atggtttaag gatttgccca ttacgaccga gcagctctac
    18421 cagcgcctga aagcacgggg cgtgctgatg gtgccggggc acaacttctt cccagggctg
    18481 gataaaccgt ggccacatac acatcaatgt atgcgcatga actacgtgcc ggagccggag
    18541 aaaattgagg cgggtgtgaa gattctggca gaagagatag aaagagcctg ggctgaaagt
    18601 cactaaaaat tgcctgatgc gctacgctta tcaggcctac atgatttctg caatatattg
    18661 aatttggtag aatttgtagg ccggataagg cgttttagcc gcatccggca taaacaaagc
    18721 gcactatccc tgcgttgaga aacgccggaa aaacgtttca aacaggctgc tgccgtagcc
    18781 gatgtcgctg cactttctcg acatccatgc actgcaacgc ctttgtcggg caggcttcaa
    18841 cacaggccgg tcccgtcgcc cgatgccagc acagatcgca cttaatcgcc ctcgcctttt
    18901 tgcgcgaaga aacgacctcc atcgcaccaa acgggcaagc cagcatacag cttttacagc
    18961 caatgcaacg tgattgttca acgaaaatat gcccatgttc gcggcttatc gcgtcaacag
    19021 ggcagacatt cgcgcacggt gcatcttcac actgatggca ggctacagcc gtggtccatg
    19081 tgtggtcttt aatgacacga atacgggaaa taaactcgtc tggtgacaac gcagcgcaat
    19141 cctgattctc ctgatgcgac actgcgcaag ccacttcaca ggtacggcaa ccgatacatt
    19201 tcgtcgcatc cgcaataata aaccggttca tgatcactcc tttgcactaa aacagcagag
    19261 tgccagagag cgcgtctcta ctgctttgat ccaacgaagg aaatgttaaa gttttggcac
    19321 ttgtccaaag cgttatgtga tcgcgccctg gtcgtccctg acagtaaatc ccagttcatt
    19381 agaaatagcc tgcgcggttt cacgcagtgg tttcaggaga tttttctccc ccacctgttt
    19441 cagacgtgat gtcgaaagcg aaatcgacac ggcgtacggc acccgcccat gaatatcaaa
    19501 caccggaaca gcaatacagg agacgccgag ttcgttttct tctctgtcca tcgccgctcc
    19561 gctttcacga atgtgcgcca gttcgtcgaa catcgcgggc agctcggtaa tggtattgcg
    19621 ggttaacggc tggatctcat gctgatggct ttcccagtat gacttcacgt agtccgggtg
    19681 gccaaacgcc atgtagatct tgcccattgc ggaacagtag agcggcatat gctggccaat
    19741 ataggcacgg gttcgcagca tcccggttgt gggttccagc ttataaatca aaatagcgtg
    19801 atcgtcttcg cggctggaga agttaatagt ttcaccagtg gcgatgttca gtgcctcaag
    19861 atgcggagcg gcgatatgaa tgatattcag cgaagacagc gccttctgcc cgacggcaat
    19921 aaatttggtg gtcaggcgat aactccctgc ggcgggcgcg gtggtcacat agccgcagga
    19981 ttgtaatccc tgcaataagc gatggacggt actcttattt aaaccagcca gctccgaaag
    20041 atgcgccaac ggacaaccgt ttggatagtt gctcaaaatc tcaatcagca tcaacccgcg
    20101 aaacagactc tggcttccgg ctggacgctc tttttcctgc gccatctcgt tctctttttt
    20161 tcccatcact tctttcccca ttttgtcgcg tcaagatggt agcgcaaagt gtgtcgtagt
    20221 tcacgatctc gacagataat ttataaccac ttgattttta agttttttga aattcatcaa
    20281 tcagattgct ttgttaaaaa gcgatcgata tatttgaaat cagatttcgc atattgaaat
    20341 tttaagccaa aaaagcgatc aaaaaaacaa ggaagccagg gatgaaagtg acatttgagc
    20401 agttaaaagc agcctttaat cgagtcttaa tttcacgcga cgttgacagc gaaacggctg
    20461 atgcctgtgc cgaaatgttc gcccgcacca ccgaatccgg cgtttattct cacggcgtca
    20521 accgtttccc tcgtttcatt caacaactgg aaaacggcga tatcattcct gatgcccaac
    20581 ccaaacgtat aaccagcctc ggcgcaattg aacagtggga cgcccagcgt tcgatcggta
    20641 acctgacagc gaaaaagatg atggatcgcg ccattgaact ggctgccgat cacggtattg
    20701 gtctggtggc actacgtaat gccaaccact ggatgcgcgg cggcagctac ggctggcagg
    20761 cggcggaaaa aggctatatt ggcatttgct ggaccaactc catcgccgta atgccgccgt
    20821 ggggcgcaaa agagtgtcgc atcggcacta acccgctgat cgtcgccatt ccttctaccc
    20881 caatcaccat ggtcgatatg tcgatgtcga tgttctctta cggcatgtta gaagttaacc
    20941 gtctggcagg ccgtcagctc ccggtcgatg gtggctttga tgatgagggc aatttgacca
    21001 aagaacctgg cgttatcgag aagaatcgcc gcattttgcc gatgggttac tggaaaggtt
    21061 cgggcatgtc gattgtgctg gatatgatcg ccactctcct ttctgacggc gcatcggttg
    21121 ccgaagtcac ccaggacaac agcgacgaat acggcatttc acaaattttt attgccattg
    21181 aagtagataa gcttatcgac ggccccaccc gcgatgccaa actgcaacgc atcatggatt
    21241 acgttaccac cgctgaacgc gctgacgaaa atcaggccat tcgcttaccc ggccacgaat
    21301 ttactaccct gctggccgaa aaccgccgta acggtattac cgttgatgac agcgtgtggg
    21361 caaaaatcca agcgttataa ggagataacc catgattttt ggacatatcg cgcagcctga
    21421 cccgtgccgt ttgcctgccg ccattgaaaa ggcgcttgat tttctgcgca ccaccgactt
    21481 caacgccctg gaaccgggcg ttgtcgaaat cgacggcaag aatatttatg cgcaaattat
    21541 cgatttaacc acacgcgaag cggcggaaaa ccgtccggaa gtgcatcgtc ggtatatcga
    21601 tattcagttt ctggcgtggg gtgaagagaa aatcggcatt gctattgata cgggaaataa
    21661 taaagtcagc gaatcactat tagagcagcg cgatattatt ttttatcacg atagtgaaca
    21721 tgaatcattt atagaaatga taccgggcag ctacgctatt ttcttcccgc aggatgttca
    21781 ccgaccaggt tgtattctgc aaacaacctc tgagatacga aaaattgtgg ttaaagtcgc
    21841 attaacagcg ctgaattaat cgaataatcg tctacgccag aacgcctg
//
LOCUS       DAZUCC010000069        21845 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZUCC010000069 DAZUCC010000000
VERSION     DAZUCC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.069"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2739
                     /locus_tag="ACHY09_002244"
     CDS             <1..2739
                     /locus_tag="ACHY09_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HFS3885213.1"
                     /translation="DSLLWNATAGAFSAKHGTNGTDSKITNLLAGTVSSDSTDAINGS
                     QLYGLADSFTSYLGGGADISDAGVLTGPTYTIGGTDYNNVGDALAAINTSFSTSLGDA
                     LLWDATAKGGDGAFSAGRGKDNTASIITNVADGAISSTSSDAINGSQLYDTSKYIADT
                     LGGDAEVNADGTITAPTYAIAGGSYSNVGDALEAIDTTLDDALLWDATANDGNGAFSA
                     AHGKDKTASVITNVANGAISATSSDAINGSQLYTTNKYIADALGGDAEVNADGSITAP
                     TYTIANAEYNNVGDALDALDDNALLWDATANDGAGAYNASHDGKASIITNVADGNIGE
                     GSTDAINGSQLFNTNMMIQQNSEIINQLAGNTSETYIEDNGAGINYVRTNDNGLAFND
                     ASASGIGATAVGYNSVAEGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIVKGSRDTSV
                     SEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVAT
                     TPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSN
                     ARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGS
                     ADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENGIGDIVSTGSTKYF
                     KTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVANEENTISVGSSTNQRRITN
                     VAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSVDYSNITLGGGNGGTTRISNVSAGV
                     NNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYT
                     PGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3151..4806
                     /gene="lldP"
                     /locus_tag="ACHY09_002245"
     CDS             3151..4806
                     /gene="lldP"
                     /locus_tag="ACHY09_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFS3885214.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4806..5582
                     /gene="lldR"
                     /locus_tag="ACHY09_002246"
     CDS             4806..5582
                     /gene="lldR"
                     /locus_tag="ACHY09_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFS3885215.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5579..6769
                     /gene="lldD"
                     /locus_tag="ACHY09_002247"
     CDS             5579..6769
                     /gene="lldD"
                     /locus_tag="ACHY09_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFS3885216.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIREFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            6842..7315
                     /gene="trmL"
                     /locus_tag="ACHY09_002248"
     CDS             6842..7315
                     /gene="trmL"
                     /locus_tag="ACHY09_002248"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS3885217.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPTEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7368..8189)
                     /gene="cysE"
                     /locus_tag="ACHY09_002249"
     CDS             complement(7368..8189)
                     /gene="cysE"
                     /locus_tag="ACHY09_002249"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFS3885218.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8269..9288)
                     /gene="gpsA"
                     /locus_tag="ACHY09_002250"
     CDS             complement(8269..9288)
                     /gene="gpsA"
                     /locus_tag="ACHY09_002250"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS3885219.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9288..9755)
                     /gene="secB"
                     /locus_tag="ACHY09_002251"
     CDS             complement(9288..9755)
                     /gene="secB"
                     /locus_tag="ACHY09_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFS3885220.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9818..10069)
                     /gene="grxC"
                     /locus_tag="ACHY09_002252"
     CDS             complement(9818..10069)
                     /gene="grxC"
                     /locus_tag="ACHY09_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFS3885221.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10211..10642)
                     /locus_tag="ACHY09_002253"
     CDS             complement(10211..10642)
                     /locus_tag="ACHY09_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS3885222.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVIVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            10887..12431
                     /gene="gpmM"
                     /locus_tag="ACHY09_002254"
                     /gene_synonym="pgmI"
     CDS             10887..12431
                     /gene="gpmM"
                     /locus_tag="ACHY09_002254"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFS3885223.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12465..13724
                     /gene="envC"
                     /locus_tag="ACHY09_002255"
     CDS             12465..13724
                     /gene="envC"
                     /locus_tag="ACHY09_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFS3885224.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13728..14687
                     /locus_tag="ACHY09_002256"
     CDS             13728..14687
                     /locus_tag="ACHY09_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFS3885225.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(14674..15708)
                     /gene="waaH"
                     /locus_tag="ACHY09_002257"
     CDS             complement(14674..15708)
                     /gene="waaH"
                     /locus_tag="ACHY09_002257"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFS3885226.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(15947..16972)
                     /gene="tdh"
                     /locus_tag="ACHY09_002258"
     CDS             complement(15947..16972)
                     /gene="tdh"
                     /locus_tag="ACHY09_002258"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS3885227.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARYVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(16982..18178)
                     /gene="kbl"
                     /locus_tag="ACHY09_002259"
     CDS             complement(16982..18178)
                     /gene="kbl"
                     /locus_tag="ACHY09_002259"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS3885228.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18392..19324
                     /gene="rfaD"
                     /locus_tag="ACHY09_002260"
     CDS             18392..19324
                     /gene="rfaD"
                     /locus_tag="ACHY09_002260"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFS3885229.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19334..20380
                     /gene="rfaF"
                     /locus_tag="ACHY09_002261"
     CDS             19334..20380
                     /gene="rfaF"
                     /locus_tag="ACHY09_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFS3885230.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20384..21364
                     /gene="rfaC"
                     /locus_tag="ACHY09_002262"
     CDS             20384..21364
                     /gene="rfaC"
                     /locus_tag="ACHY09_002262"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFS3885231.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKQFIEENAEKAAMI"
     gene            complement(21434..>21845)
                     /locus_tag="ACHY09_002263"
     CDS             complement(21434..>21845)
                     /locus_tag="ACHY09_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS3885232.1"
                     /translation="GARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPF
                     VDSHLHNDLIDTLSTRGIPGVALTILAFSAIFIYALRTAKEPYILILLFSLLVVGLSD
                     VILFSKPVPTAVFVTIILLCAYFKAQSDQCLLDK"
BASE COUNT         5351 a         5740 c         5688 g         5066 t
ORIGIN      
        1 gattccctgc tgtggaacgc tacggcgggt gcattcagtg ccaaacacgg caccaacggt
       61 actgacagca aaatcaccaa cttactggca ggcactgtat cctctgatag cactgacgcc
      121 attaatggct cacaacttta tggtttagct gattcattta cgtcatacct tggcggtggt
      181 gctgatatca gcgatgcggg tgtattaacc gggccaacct acactattgg tggtactgac
      241 tacaataacg tcggtgatgc tctggcagcc attaacacgt catttagcac atcactcggt
      301 gacgccctac tctgggatgc gaccgccaag ggcggtgacg gtgcgtttag cgccggtcgc
      361 ggaaaagata acacggccag catcattact aatgttgcag acggtgcaat ttcctctacc
      421 agcagcgacg ctattaacgg ctcacagctc tatgacacca gtaagtacat tgccgatact
      481 ctgggtggtg acgcagaagt caatgctgac ggcacaatca ccgcaccgac ttatgctatc
      541 gctggcggca gttacagcaa cgtcggtgac gcgctggaag caatcgatac cacgctcgat
      601 gacgctctgt tgtgggatgc cacagccaat gatggtaatg gtgcatttag cgccgctcac
      661 ggaaaagata aaacagccag tgtaatcact aacgtcgcta acggtgcaat ctctgccacc
      721 agcagcgatg ccattaacgg ttcacaactg tataccacca acaagtacat tgccgatgcc
      781 ctgggtggtg acgcagaagt taacgctgat ggttctatca ctgcgccgac ttacaccatt
      841 gcaaatgccg agtacaacaa cgtcggtgac gccctggatg cgctcgacga taacgctctg
      901 ctgtgggatg caacggccaa tgacggcgca ggtgcctaca acgccagcca tgacggcaag
      961 gccagcatca tcacaaatgt tgctgatggt aacattggcg aaggcagtac cgacgccatc
     1021 aacggttctc agctgtttaa caccaatatg atgatccagc agaacagcga aatcatcaat
     1081 cagcttgccg gtaacaccag cgaaacctac atcgaagata acggtgcggg tattaactat
     1141 gtacgtacca acgacaacgg cttagcgttc aacgatgcca gcgcttcagg tattggcgct
     1201 acggctgtgg gttataactc tgttgccgaa ggcgatagca gcgtagccat cggtcagggc
     1261 agctacagcg acgttgatac cggtattgcc ctgggtagca gctccgtttc cagccgtgta
     1321 atagttaaag gttctcgtga caccagcgtg tcggaagaag gtgttgtgat tggttatgac
     1381 accacggatg gcgaactact tggtgcgctg tctattggtg atgacggtaa atatcgtcaa
     1441 atcatcaacg tagccgatgg ttccgaagcc catgacgccg ttacggttcg ccagttgcaa
     1501 aatgccattg gtgcagtcgc taccacgccg accaaatact tccacgccaa ctcaacggct
     1561 gaagactcac tggctgtcgg ggaagactcg ctggcgatgg gcgcgaaaac tatcgtcaat
     1621 ggtaatgcgg gtattggtat cggcctgaat actctggttc tggcagatgc catcaacggt
     1681 attgctatcg gttctaacgc acgcgccaac catgcaaaca gtattgcaat gggtaatggt
     1741 tctcagacca ctcgcggcgc tcagactggc tacaccgcct acaacatgga tgcaccgcag
     1801 aactctgtgg gtgaattctc cgtaggtagc gaagacggtc aacgtcagat caccaacgtc
     1861 gcagcggggt cggcggatac cgatgcggtt aacgtgggtc agttgaaagt aacggacgcg
     1921 caggtttccc agaataccca gagcattact aacctgaata ctcaggtaac gaatctggat
     1981 acccgcgtca ccaatatcga aaacggtatt ggcgatatcg taagcaccgg tagcaccaag
     2041 tacttcaaga ccaacaccga tggcgtagat gctaacgcgc agggtaaaga cagtgttgcg
     2101 attggctctg gctctattgc tgccgctgat aacagcgttg cgctgggtac tggctctgtg
     2161 gcaaacgaag aaaatacgat ctccgtaggt tcttccacta accaacgccg tatcaccaac
     2221 gttgccgctg gtaaaaatgc tactgacgcg gttaacgttg cgcagttgaa gtcttccgaa
     2281 gcaggcggcg tgcggtatga caccaaagct gacggttctg ttgattacag caacatcact
     2341 ctcggcggtg gtaatggcgg aacgactcgc atcagcaacg tttccgctgg cgttaacaac
     2401 aacgacgcgg tgaactacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc
     2461 gatcagcgca tggttgagat ggataacaaa ctgtccaaaa ccgaaagcaa gttgagcggt
     2521 ggtatcgctt ctgcaatggc gatgaccggt ctgccgcagg cttacacgcc aggtgccagc
     2581 atggcctcta ttggtggcgg tacttacaac ggtgaatcgg ctgttgcttt aggtgtgtcg
     2641 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
     2701 gaatactccg ccgcgctcgg tgccggtatt cagtggtaat catccattaa cgaatgggta
     2761 aacgccacat tgcctgatgc gctacgctta tcaggcctac ggggtgattc agcactaccc
     2821 tgtaggccgg ataaggcgtt cacgccgcat ccggcaagat aaggcgcacc agatcaacaa
     2881 tctgagggca attctctgat gggaattgcc cttttcttca ttacctctct cccagaattg
     2941 gccctaccaa ttcttcattg atctgacctc tggttcacaa ttccccaatt aaaactcaca
     3001 tcaccgttgc caatacataa catctagtta actattcatt gtcattatcc ctacacaaca
     3061 caattggcag tgccactttt acacaacgtg tgacaaggag atgagcaaca gactcattac
     3121 acggcgtgtg tggactgcag gagacctgca atgaatctct ggcaacaaaa ctacgatccc
     3181 gccgggaata tctggctttc cagcctgata gcatcgcttc ccatcctgtt tttctttttt
     3241 gcgctgatta agctcaaact gaaaggatac gtcgccgcct catggacggt ggcaatcgcc
     3301 cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc ctcggtgatt
     3361 tatggcttct tctacggttt gtggcccatc gcgtggatca ttattgcggc agtgttcgtc
     3421 tataagatct cggtgaaaac cgggcagttt gacatcatcc gctcgtctat tctttcgata
     3481 acccctgacc agcgcctgca aatgctgatc gtcggtttct gtttcggcgc tttccttgaa
     3541 ggagccgcag gctttggcgc tccggtagca attaccgccg cattgctggt cggtctgggc
     3601 tttaaaccac tctacgccgc cggactgtgc ctgattgtta acaccgcgcc agtggcattt
     3661 ggtgcgatgg gtattccgat tctggtcgcc gggcaggtaa caggcatcga cagctttgag
     3721 attggtcaga tggtggggcg tcagttgccg tttatgacca ttatcgtgct gttctggatc
     3781 atggcgatta tggacggttg gcgcggaatc aaagaaacgt ggcctgcggt cgtggtggca
     3841 ggtggttcgt ttgccattgc tcaatacctc agctctaact tcattgggcc ggaactgcca
     3901 gacattatct cttcgctggt atcactgctc tgcctgacgc tgttcctcaa acgctggcaa
     3961 ccagtgcgcg tattccgttt cggtgacttg ggggcgtcac aggttgatat gacgctggct
     4021 cacaccggtt acaccgcagg tcaggtgctt cgcgcctgga caccgttcct gttcctgacc
     4081 gccaccgtaa cgctgtggag tatcccgccg tttaaagccc tgttcgcttc aggcggtgcg
     4141 ctgtatgagt gggtgatcaa cattccggtg ccgtacctcg ataaactggt tgcccgtatg
     4201 ccgccagtgg tcagcgaagc gacagcgtat gccgccgtgt ttaagtttga ctggttctct
     4261 gccaccggta ccgccattct ttttgctgcc ctgctctcaa ttgtctggct gaagatgaaa
     4321 ccatctgacg ctatcagcac cttcggcagc acgctgaaag aactggctct gcctatctac
     4381 tccatcggta tggtgctggc gttcgccttt atctcgaact attccggact atcatcaacg
     4441 ctggcgctgg cactggcgca caccggccat gcattcacct tcttctcgcc gttcctcggc
     4501 tggcttggtg tcttcctgac cggatcggat acctcatcta acgccctgtt cgccgccctg
     4561 caagccactg cagcacaaca aattggcgtt tctgacctgt tgttggttgc cgccaacacc
     4621 accggtggtg tcaccggtaa gatgatctct ccgcaatcta tcgctatcgc ctgtgcggcg
     4681 gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca cagcctgatc
     4741 ttcacctgta tggtgggcgt gatcaccacg cttcaggctt atgtcttaac gtggatgatt
     4801 ccttaatgat tgttttaccc agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc
     4861 tgattgatga aaaaaacctg gaagcgggca tgaagttgcc cgctgaacgt caactggcga
     4921 tgcaactcgg cgtgtcgaga aattcactgc gcgaggcgct ggcgaaactg gtcagtgaag
     4981 gtgtgctgct cagtcgacgt ggcggcggga cgtttattcg ctggcgtcat gatacatggt
     5041 cggagcaaaa catcgtccag ccgctgaaaa cgctgatggc cgatgatccg gattacagtt
     5101 tcgatattct ggaagcccgc tacgccattg aagccagcac cgcgtggcat gcggcaatgc
     5161 gcgccacacc aggcgaaaaa gagaaaattc agctttgctt tgaagcaacg ctaagtgaag
     5221 acccggatct cgcctcacaa gcggacgttc gttttcatct ggcgattgcc gaagcctcac
     5281 ataacatcgt gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa tcctctgtga
     5341 agcatagccg ccagcggatg tatctggtac ccccggtttt ttcacagctg accgaacaac
     5401 atcaggcagt cattgacgcc atttttgccg gtgatgctga cggggcgcgt aaagcaatga
     5461 tggcgcacct tagctttgtt cacaccacca tgaaacgatt cgatgaagat caagctcgcc
     5521 atgcacgcat tacccgcctg cctggtgacc ataatgagca ttcgagggag aaaaacgcat
     5581 gattatttcc gcagccagcg attatcgcgc cgcagcacaa cgcattctgc cgccgttcct
     5641 gttccactat atggatgggg gtgcatattc tgaatacacg ctgcgccgca acgtggaaga
     5701 tttgtcagaa gtggcgctgc gccagcgtat tctgaaaaac atgtctgact taagcctgga
     5761 aacgacgctg tttaatgaga aattgtcgat gccggtggcg ctaggtccgg taggtttgtg
     5821 tggcatgtat gcgcgacgcg gcgaagttca ggctgccaaa gccgcggatg cgcatggcat
     5881 tccgtttact ctctcgacgg tttccgtttg cccgattgaa gaagtggctc cagccattaa
     5941 acgtccgatg tggttccagc tttatgtgct gcgcgatcgc ggctttatgc gtaacgcact
     6001 ggagcgagca aaagcggcag gctgctcgac actggttttc accgtggata tgccgacacc
     6061 gggcgcgcgt taccgtgatg cacactcggg tatgagcggc ccaaatgcgg caatgcgccg
     6121 ttacttgcag gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga acggtcgtcc
     6181 acatgattta ggtaatatct cggcttacct cggcaaaccg accggactgg aagattacat
     6241 cggctggctg gggaataact tcgatccgtc catctcatgg aaagaccttg agtggatccg
     6301 cgaattctgg gatggtccga tggtgatcaa agggatcctc gatccggaag atgcgcgcga
     6361 tgcagtacgt tttggtgctg atgggattgt ggtttctaac cacggtggcc gccagctgga
     6421 cggtgtactc tcttccgctc gtgcattgcc cgctattgcg gatgcggtga aaggtgatat
     6481 tgccattctg gcagatagcg gaatacgtaa cgggcttgat gtcgtgcgta tgattgcgct
     6541 cggtgccgac accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa cagcgggcca
     6601 ggcgggcgtg gcaaatctgc tgaatctgat cgaaaaagag atgaaagtgg caatgacgct
     6661 gactggcgcg aaatcgatta gcgaaattac gcaagattcg ctggtgcagg ggctgggtaa
     6721 agagttgccg gcggcactgg ctccgatggc gaaagggaat gcggcatagt tgggggtgag
     6781 gggaaaaggc cgtaccaatc accaattttc tgctatcctg cccccgcact aagggggcag
     6841 tatgctaaac atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg
     6901 tctttgcgct aataccggct ttcgtctgca tatcatcgaa ccaatgggat ttgcctggga
     6961 cgataagcgc ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca
     7021 tcatgactat cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac
     7081 gaaaggtacg cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg
     7141 cccggaaaca cgcggcctgc cagcgagcat tcttgatgcc ctgcccactg aacaaaaaat
     7201 tcgtattcca atggtgccgg acagccgcag catgaacctg tctaatgcgg tgtcggtcgt
     7261 ggtgtatgaa gcctggcggc agttggggta tccgggagcg gtgttgagag attagttact
     7321 gtaggccgga tagatgatta catcgcatcc ggcacgatca caggacatca gatcccatca
     7381 ccatactcaa acgtatggtt aatgccgttg aaatgctgat ccatatccat tgacggttta
     7441 tcgctatccg gcttgccgac aatgcgtgcc ggaacgccag cggcagtggt gtgcggcggc
     7501 accggttgca gcaccacgga acctgcgcca atcttcgcgc cgcgcccaac ttcaatatta
     7561 ccgagaattt tcgcgcccgc gccaatcatc acgccttcac gaattttcgg gtgacggtca
     7621 ccaccagatt tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca
     7681 atcaccgccg tttcacccac aacgatgccc gtcgcgtggt cgagcatgat accgcgacca
     7741 atttttgctg ccgggtgaat atcaacctgg aacgttacag agacctggtt ttgtagaaaa
     7801 atagccagcg cgcgacggcc ctgattccac aaccagtgac cgatgcgata cgcctgcaaa
     7861 gcatgaaaac ccttcaggta tagcaacggg gttgagtatt tatccaccgc cgggtcacgg
     7921 gtacgcaccg cctgaatatc acaggccgca gaggcgatca tttccgggtc ggcggcgtag
     7981 gcttcttcca ccacttcacg gatagcaata gccggcataa tgggcgatga cagcttgttc
     8041 gccagcatgt agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa
     8101 aaactggcca gcattggctc acagtctgcc agcgttctgg cttcagcttt aatattgttc
     8161 cagacaattt ccagttcttc acacgacatt gcttactcca cacgataaga taatgatcgg
     8221 cccgttctgc gcgggccggg tcatagtggt aacaaaggtt ccctgggttt agtggctgct
     8281 gcgctcgtcc ttgcgtgcac gacctagtaa agtcaatgct gcctcgcgcg cgttttttcc
     8341 gcaatataat acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc
     8401 cagttcgcgg acttctttcg tattgcggta gccttccacc acttgaccaa ttttctcctg
     8461 cgcgctttgt acatccatgc cctgaccgag catcatgcca aaacggcggt tacgcgactg
     8521 gttgtcggta caggtcagca ccagatcgcc cagccccgcc atgcccataa aggtggcagg
     8581 atcggcaccc agcgccgcgc caagacgcga catctcagcc agcccacggg tgatcagcgc
     8641 cgtacgcgca ttcgcgccaa aaccgatacc gtcggacatc cccgcgccaa tggcaatgac
     8701 gtttttcacc gcgccgccaa gctgcacacc gatgaaatcc gggttgctgt aaacgcggaa
     8761 acttttgcca cagtgcagca attgctggag gtcatcggca aaggtctggt ccgtcgaggc
     8821 cagcgaaatg gctgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat
     8881 aaccgccagc ggaatttgat cgcctaaggc ctcacgcgct acgtcctgta acagacgtcc
     8941 ggtttctgct tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg
     9001 tttaatctgg cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg
     9061 gctggctgcc agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac
     9121 atcggggaga aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc
     9181 atggccccag aggacaacct cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc
     9241 gtacgagccg gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga
     9301 tgttcttcag taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag
     9361 ttaaccggcg caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac
     9421 tcacgagcat acgggaacag aatgttcggg cagtatgcac ccaggcaatg tgccatctgg
     9481 gtgccttcga tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg
     9541 gtttcttcgc ccagggaggc cgttacggta acacgcagta ccacttcgta aacgtcatct
     9601 gccagttgag aagatgccgt atccagatca agtttaactt ctggctgcca atctttctgg
     9661 aaaacgtgcg gcgcgttcgg cgcttcgaaa gaaatatcct tggtataaat acgttggatc
     9721 tggaaagtca tttcggtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc
     9781 cttaaatact gcgcaatgta atgccaacgt tcacacgtta tttcagcagg ggatccagtc
     9841 caccacgtgc atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa
     9901 aaatctgggg aaccgtggtg cgaccgctgc gtttgatcat ctcttcacgc ttagcggcgt
     9961 tgccatcgat cggcaactcc tgaaaactca cgcccttgct gctcagcagt gctttcgcac
    10021 gatggcaata cgggcaggtt tctttggtat agatttcaac attggccata acttagctcc
    10081 tgttttttta ccctgaaatt tcatgttgca gggcggctgc aaaatggagt gtccccagaa
    10141 gcttataaca ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac
    10201 gacaggtaaa ttatttgccg cgcaccagag gtaagttttc gccagcccag ccagcaacgc
    10261 cctctttcag cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct
    10321 ggcactgcat gccagaaccg tctaccacga taaccggttt gtctttgtgc ttctcaagct
    10381 caccaacatt gttggctttg atttcgctcg gcaacaggtt gatagaacct gcgatatggc
    10441 ctttacggaa gtcatcacgc tgacgtaaat ccacaaccac agcatcttct ttgttgatca
    10501 gacgcgtagc ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag
    10561 tcacaataac cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa
    10621 caaattgcat aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc
    10681 aggaaaggag tatacctgcg cggtgcagca aatacagcca gcgcgttaac tggaatgcaa
    10741 ttttgcgggg cgcgacgaaa aaaaagcgca aatctggcat cggcttgctg cgagcggtcg
    10801 tatattttga tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt
    10861 tgactcttga gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga
    10921 ttctggatgg ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa
    10981 ccccggtaat ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc
    11041 tggaagtcgg tctgcctgac cgtcagatgg gtaactccga agtaggtcac gttaacctgg
    11101 gtgccggccg catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg
    11161 ctttcttcgc taacccggta ctgactggcg cggtagataa agcgaaaaac gcaggcaaag
    11221 cagttcacat tatgggtctg ctctccgcag gtggtgtaca cagccacgaa gatcacatca
    11281 tggcgatggt agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc
    11341 ttgacggtcg cgacactccg ccgcgcagtg ctgaatcctc gctgaaaaaa ttcgaagaga
    11401 aatttgccgc actgggcaaa ggccgcgtag cgtccatcat tggtcgttac tacgccatgg
    11461 accgcgataa ccgttgggat cgcgttgaaa aagcttatga cctgctgact ctggcgcagg
    11521 gcgagttcca ggccgatacc gccgttgctg gtttgcaggc tgcttatgct cgcgacgaaa
    11581 acgacgaatt cgtgaaagcg accgttattc gtgcagaagg tcagccagac gcggcaatgg
    11641 aagacggcga tgcgctgatt ttcatgaact tccgtgctga ccgtgcacgt gaaatcactc
    11701 gtgcattcgt aaatgccgat tttgacggct tcgcgcgtaa gaaagtggtt aacgtcgatt
    11761 tcgtgatgct gaccgaatac gctgccgaca tcaaaaccgc agtggcttac ccacccgctt
    11821 ccctggttaa caccttcggc gagtggatgg cgaaaaacga caaaactcag ctgcgtattt
    11881 ccgaaaccga aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt
    11941 tcaaaggcga agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac
    12001 cggaaatgag ctccgcagag ctgaccgaaa aactggttgc ggcaatcaag agcggcaaat
    12061 acgacaccat tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggagtgatgg
    12121 aagcggcggt taaagcggtt gaagcgctgg atcactgcgt ggaagaagtc gcgaaagcgg
    12181 ttgagtccgt gggtggacaa ctgctgatca ccgctgacca cggtaacgct gagcagatgc
    12241 gcgatccggc aacgggtcag gcacacacgg cacataccaa cctgccagtt ccgctgattt
    12301 acgttggcga taagaacgtg aaagcggttg aaggcggcaa actttctgat atcgcgccga
    12361 ccatgttgtc gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca
    12421 tcgtggaata atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga
    12481 aaccgcgcag gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat
    12541 tgttgtgcgc cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg
    12601 atatcgccgc gaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg
    12661 cacaattgaa aaagcaggaa gaagcgatct cggaagccac ccgtaagctg cgtgaaacgc
    12721 aaaacacgct caatcaactg aataaacaga ttgatgagat gaacgcgtcg attgccaaac
    12781 tggagcagca aaaagccgcc caggagcgca gcctcgccgc acaactagat gccgcattcc
    12841 gtcagggcga gcataccggt attcagctga ttctcagcgg tgaagaaagc cagcgtggac
    12901 agcgtttaca ggcttatttc ggctatctca accaggcgcg acaagaaacc attgctcagt
    12961 tgaaacaaac gcgtgaagaa gtcgctatgc aacgcgccga gctggaagag aaacagagcg
    13021 agcaacaaac gctgttgtat gagcagcgcg cccaacaggc gaagctgacc caggcgctga
    13081 acgagcgtaa aaagacgctg gcagggctgg agtcttccat ccagcaaggt cagcaacagt
    13141 tgagcgagct gcgcgccaac gaatcccgtc tgcgtaacag cattgcccgt gcggaagccg
    13201 cggcgaaagc gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc gaccgccaga
    13261 aagaagcgac gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt
    13321 cccgaactgg tggcctgggt gcgccgcgtg gtcaggcatt ctggccagtt cgcgggccga
    13381 cgctgcatcg ctatggcgag cagctacagg gtgaattacg ctggaaagga atggttatcg
    13441 gtgcctctga aggtactgaa gttaaagcca ttgccgacgg tcgggtgatt ctggctgact
    13501 ggctgcaagg ctacggtctg gtggtggtgg ttgagcatgg taaaggcgac atgagtcttt
    13561 acggctataa tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccga
    13621 ttgcactggt gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc
    13681 gccagggtca ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc
    13741 gtcgtaacgt tcttgcattt gccgctctgt tggcgctctc ctcccccgta attgctggca
    13801 agcttgccat cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg
    13861 cgatgccttc cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg
    13921 caaccaaagc gcataacagc ggtcacgaag tgttgattca tctaccgatg gcaccactga
    13981 gtaaacagcc gctggagaaa aatacgctac gcccggagat gagcagcgac gaaattgagc
    14041 gcattattcg tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg
    14101 gcagcaagat gacctctaac ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt
    14161 acaatcttta cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgcagcgc
    14221 aaggcactgg cgtgaaggtg atcaaacgga aagtgttcct cgacgattcg caaaatgaag
    14281 cggacatccg tgtgcagttc aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca
    14341 ttgccattgg gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc
    14401 tgccgccaga cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata
    14461 cgtctacacc gccgaaaaac actgtgcccg acacaccgcg taatccgttc cgtggcgtga
    14521 agctctgcaa accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat
    14581 taagcgaaag catcagccag agcacgctga tcgtttactt ccaacatcag tggcaaggct
    14641 ggggcaaaca gcctgaagcg gcaaaactta acgctagcgt aaattaaagg cgctacgcgt
    14701 aatgcgatgg tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa
    14761 cgcctgataa cccaccttca cgctgcgcac attggtaatc aggcgcttat acataccgga
    14821 agtaaatatc tcggcaatca tccgttggcg ggtaataata tccggctctt tgcgcaccgc
    14881 atggcaaaca cgcaatgctt cgtaagttat ttgctgatga aattccggat aaatcgtaat
    14941 tttgtcggca taatttcgat ttaatttctc tagcaggcgg gtaatcttaa tatagtgacg
    15001 ttgataatta aggtttttat tcccttgtct attcaaccga ctcactgacg tattatgcag
    15061 ataatattta tataacgatt gctcggtata tcgcgcacgc agcgcattaa acataaattc
    15121 tgttgtccag acaatatcct gatgatgtaa tccggcaata aatttaatgt tatttttaac
    15181 aataacatcg cggcgataaa cccccatcca gacaacgtga gtccaacggc gggaagaaag
    15241 ccccatccgc aaccaatccg ggccggttaa tacgccggtt gagcgaaggc gatcggtggg
    15301 gatggattgc caggtttctc ccgtttcacg aaaacaccag tcagcgttgc actgcgccac
    15361 gtcgaggtcg tcctctaacg ccatggtcat cagcgtttcg tacatggtgg gatagacctc
    15421 atcgtcagca tcgacaaaag cgacatattt gcccgtcgcc acctcgatcc cacgattacg
    15481 cgctaccgat gcgccagcat tcgcctgatg caacaaacga acgtgcggat agttttctgc
    15541 gtaatgcttt gctatttcaa cagaattatc cgttgaacca tcgttaataa taatgatttc
    15601 cagagcagtc caggtttgcg taattaaaga ttccatacac gtgcggaaat catcgcccgc
    15661 attatataac gggataataa cactaagttt attggtgctg ttcatcataa attaccgacg
    15721 acctctgaag ttatcctgac gttttacagg ctttgtatta agaaactatt aagcctgtga
    15781 gatattattt ttacaggtga gagagtcact tcagggtaga gagataatga gaacagctat
    15841 ataagagatc agcgcaatta cgaactcttt attacactga caggatttta catatttaac
    15901 ggattatcct caggaaaaag ggctggaata ccagcccttg tttgtgttaa tcccagctca
    15961 ggataacttt cccggactgg cccgaacgca tggcgtcaaa gcccttctgg aaatcatcga
    16021 tagagaaacg atgagtaatg atcggcgaaa gatcgaggcc agactgaatc agcgccgcca
    16081 tcttgtacca ggtttcaaac atctcacgac cgtaaatacc tttaatgaac aagcctttaa
    16141 agatcacttt ggtccagtct atcgacatgt cagaaggtgg aatacccagc atcgcaatac
    16201 gaccgccgtg gttcatggtg tcaagcatgg tacgaaacgc tggcggcgca ccggacattt
    16261 ccagaccgac atcaaagcct tcggtcatgc ccagttctgc catcacatca ttgaggttct
    16321 ctttagcgac gttaaccgca cgggtgatac ccattttacg cgccaactca aggcggtatt
    16381 cgttaacatc agtaatcacc acgtaacgtg caccaacgtg tttcgccacc gccgccgcca
    16441 taataccaat cgggcctgca ccagagacca gcacatcctc gcccaccaga tcgaacgaca
    16501 gcgcggtatg cacggcgtta ccgaagggat caaaaatgga agccagatca tcggagatat
    16561 tgtcggggat tttgaaagcg ttgaacgccg gaatcaccag atactcggcg aagcagcccg
    16621 gacggttaac gccgacgccg atcgtgttgc ggcacagatg ggtacgaccg ccacgacagt
    16681 tgcgacagtg accgcaggtg atatggcctt cgccagaaac gcgatcgcca atcttgaagc
    16741 ctttcacttc ctgaccaata cctaccactt caccgacata ttcatgaccc acgaccatcg
    16801 gcaccgggat ggttttttgc gaccactcat cccagttata gatgtgaacg tcagtcccgc
    16861 agatggctgt tttacggatt ttaatcagca gatcgttatg cccgagttcc ggtacaggaa
    16921 cgtcggtcat ccagatgccc tcttccgctt tcagtttgga taacgctttc atctcacatc
    16981 ctcaggcgat aacgcccagt tgtttaccaa tacgcgtgaa tgcttctact gcacgcgtaa
    17041 tttgctcagg ggtatgcgcc gcagacatct gggtacgaat acgcgcctga cctttcggaa
    17101 cgaccggata gaagaaaccg gtgacgtaaa tcccctcttt ttgcagctca cgggcaaatt
    17161 tctgcgccac taccgcatcg ccaagcatga ccggaataat ggcgtgatcg gctcccgcca
    17221 gggtaaagcc cgccgccgac atttgctcac ggaactgacg cgcgttcgcc cacagacggt
    17281 cacgcagctc gctacccgct tcaaccatct ccagcacttt gatggaagcg gcaacaatgg
    17341 ccggtgccag cgagttggag aacaggtacg gacgagaacg ctggcgcagc cactcaacca
    17401 cttctttgcg cgccgcggta taaccaccag aagccccgcc cagcgcttta ccaagcgtac
    17461 cggtgataat atcgacccgc cccatcacat cgcagtattc atgggaacca cgaccgtttt
    17521 caccgacaaa acctaccgcg tgtgagtcat ctaccatcac cagggcatca tatttatctg
    17581 ccagatcgca aacgcctttc aggttggcaa tcacgccgtc catcgagaac acaccatcgg
    17641 tggcaatcag cacatgacgc gcaccggctt cacgcgcttc tttcagacgt gcttccagct
    17701 cctgcatatc gttgttggca taacgatagc gtttagcttt gcacagacgc acaccatcaa
    17761 taatagacgc gtggttcagt gcgtcggaga taatggcgtc ttccgcgccc aacagcgttt
    17821 cgaacaggcc accgttagca tcaaagcagg aagagtagag aatcgcatct tccatcccca
    17881 ggaaggccgc cagtttttgt tcaagctctt tatggctgtc ctgagtgccg caaataaaac
    17941 gcaccgaagc catgccgaaa ccgtgagaat ccattcccgc ctttgccgcc gcaatcagat
    18001 ccggatgatt cgccagtccg agatagttat tggcacaaaa gttaatgacg tggcttccat
    18061 cagccacagt gatatctgct tgctgcgcag acgtaataat gcgctcttct ttaaacaacc
    18121 cttccgcccg tgcggtttcc agatcgttgg ttaactgctg ataaaattct ccacgcattg
    18181 cgattctcca gacagggcaa attccagcac atattaccca aacttatagg ttgcgacgag
    18241 atgacgcgtt aacacttctg caaaattcag gataaataac gtccagcccc ctcacttttg
    18301 gtgaatggct gaagccaacg tcatgtagtg atatgataga agtattcgtg tctgagattg
    18361 tctctgactc cataattcga aggttacagt tatgatcatc gttaccggcg gcgcgggctt
    18421 tatcggaagc aacatcgtta aagccctgaa tgataaaggc atcaccgata ttctggtggt
    18481 ggacaacctg aaagacggca ccaagtttgt gaacctggtg gatctggata tcgcggacta
    18541 tatggataag gaagacttcc tgatccagat tatggctggc gaagagttcg gcgatgtcga
    18601 agcgattttc cacgaaggcg cgtgctcttc caccaccgag tgggacggca agtatatgat
    18661 ggataacaac tatcaatact ccaaagagct gctgcactac tgtctggagc gcgaaatccc
    18721 gttcctgtat gcctcttccg cagccactta cggtggacgc acctccgact ttattgaatc
    18781 ccgcgaatat gaaaaaccgt tgaatgtcta cggttactca aaattcctgt ttgatgaata
    18841 tgttcgccaa atcctgcctg aagcgaactc acagattgtt ggcttccgtt atttcaacgt
    18901 ttatggaccg cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc atctcaacac
    18961 ccagcttaat aacggtgaat caccgaagct gttcgaaggt agcgagaact tcaaacgtga
    19021 cttcgtctat gtgggcgatg tggcagatgt aaacctgtgg ttcctggaaa atggcgtttc
    19081 cggcatcttc aacctcggta ctggtcgtgc ggaatccttc caggcagtag cagatgctac
    19141 gcttgcttat cacaagaaag gccaaatcga atacattccg ttcccggata aactgaaagg
    19201 ccgctaccag gcgttcacgc aggcagatct gacaaatctg cgcgcggcgg gttacgacaa
    19261 accgtttaaa accgttgccg aaggcgtaac ggaatacatg gcctggctga atcgtgacgc
    19321 ataagagctc tgcatgaaaa tactggtgat cggcccgtct tgggttggcg acatgatgat
    19381 gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc caggcgataa tcgacgtgat
    19441 ggcaccggca tggtgccgtc cattattatc gcggatgccg gaagttaacg aagctattcc
    19501 tatgcctctc ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg gtcatagcct
    19561 gcgcgaaaaa cgttacgacc gcgcctacgt tttacccaac tccttcaaat ccgcattagt
    19621 gcctttcttc gcgggtattc ctcatcgcac cggctggcgc ggcgaaatgc gctacggttt
    19681 actcaacgat gtgcgtgtgc tggataaaga agcctggccg ctgatggtgg aacgctatgt
    19741 ggcgctggcc tatgacaaag gtattatgcg taccgcacaa gatctgccgc agccattgtt
    19801 atggccgcag ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc aattttcgct
    19861 ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg gagtttggtc cggcaaaacg
    19921 ctggccacac taccactatg cggagctggc aaagcaactg attgatgaag gttatcaggt
    19981 ggttctgttt ggctcggcga aagatcatga agcgggcaat gagattcttg ccgctttgaa
    20041 taccgagcag caggcatggt gtcgtaacct ggcgggggaa acacagcttg atcaagcggt
    20101 tatcctgatt gcagcctgta aagccattgt tactaacgat tctggcctga tgcacgttgc
    20161 ggcggcgctc aatcgtccgc tggttgccct gtatggtccg agtagcccgg acttcacacc
    20221 gccgctatcc cataaagcgc gcgtgatccg cctgattacc ggctatcaca aagtgcgtaa
    20281 aggtgacgct gcggagggtt atcaccagag cttgatcgac attactcccc agcgcgtact
    20341 ggaagaactc aacgcgctat tgttacaaga ggaagcctga cggatgcggg ttttgatcgt
    20401 taaaacatcg tcgatgggcg atgttctcca tacgttgccc gcactcactg atgcccagca
    20461 ggcaatccca gggattaagt ttgactgggt ggtggaagaa gggttcgcac agattccttc
    20521 ctggcacgcc gccgttgagc gagttattcc tgtggcaata cgtcgctggc gtaaagcctg
    20581 gttctcggcc cccataaaag ctgaacgcaa agcgtttcgt gaagcgctac aagcagagaa
    20641 ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc gcggcgctgg tgacgcgtct
    20701 ggcgcatggc gtaaagcatg gcatggactg gcaaaccgct cgcgaacctt tagccagcct
    20761 gttttacaat cgtaagcatc atattgcaaa acagcagcac gccgtagaac gcacccgcga
    20821 actgtttgcc aaaagtctgg gctacagcaa accgcaaacc cagggcgatt atgctatcgc
    20881 acagcatttc ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc
    20941 gacgacccgt gatgataaac actggccgga agaacactgg cgagaattga ttggtttact
    21001 ggctgattca ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc
    21061 gaaacgactg gcggaaggat ttgcttatgt tgaagtattg ccgaagatga gtctggaagg
    21121 cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg ggttaagcca
    21181 tttaacggcg gcactggata gacccaatat cacggtttat ggacctaccg atccgggatt
    21241 aattggtggg tatgggaaga atcagatggt ttgtagggct ccggggaatg agttgtctca
    21301 attgacagca aatgctgtta agcaattcat tgaagaaaac gctgaaaaag ctgccatgat
    21361 ttaaattaca actctttaat agataatttt gaaataaaat cagcttcctt gctgatttta
    21421 tttcacatat tcattactta tctaataaac attggtccga ttgtgcttta aaataagcac
    21481 aaagcaatat tatggtgaca aacacagcag tcggaaccgg tttagaaaag agtattacat
    21541 cacttaggcc tactaccagt agtgaaaaaa gcaagattaa aatataaggt tctttagcag
    21601 ttcttaaggc atatatgaat attgctgaaa aggctaaaat tgttaatgct actccaggaa
    21661 taccacgcgt tgataacgta tctatgagat cgttatgcaa atgagaatct acaaagggta
    21721 aagcgccact taatctaggc tctttctctt ctagttcatg tattttttcc gcacgttttt
    21781 ccagtgattg tcctattgga gaatatgttt ttaaaccaac ttcatacatt gccagacggg
    21841 caccg
//
LOCUS       DAZUCC010000070        21375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZUCC010000070 DAZUCC010000000
VERSION     DAZUCC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.070"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..832
                     /locus_tag="ACHY09_002264"
     CDS             <1..832
                     /locus_tag="ACHY09_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFS3885233.1"
                     /translation="FVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTRKLESIFNNTQV
                     QIAVLIIPTTKDETIEQYATRVFNNWRLGDAKRNDGILIIVAWSDRTVRIQVGYGLEE
                     KVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYSANPSESESASSS
                     DHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDLFLFSDKTFSIAVF
                     SFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSARGSGGGFSGGGGSSGGGGASGRW
                     "
     gene            complement(892..2190)
                     /gene="eno"
                     /locus_tag="ACHY09_002265"
     CDS             complement(892..2190)
                     /gene="eno"
                     /locus_tag="ACHY09_002265"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS3885234.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2278..3915)
                     /gene="pyrG"
                     /locus_tag="ACHY09_002266"
     CDS             complement(2278..3915)
                     /gene="pyrG"
                     /locus_tag="ACHY09_002266"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFS3885235.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4143..4934)
                     /gene="mazG"
                     /locus_tag="ACHY09_002267"
     CDS             complement(4143..4934)
                     /gene="mazG"
                     /locus_tag="ACHY09_002267"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFS3885236.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     GSAGNSREVLARWEQIKTEERAQKAQHSALDDIPSSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQVVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5030..7264)
                     /gene="relA"
                     /locus_tag="ACHY09_002268"
     CDS             complement(5030..7264)
                     /gene="relA"
                     /locus_tag="ACHY09_002268"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFS3885237.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7312..8613)
                     /gene="rlmD"
                     /locus_tag="ACHY09_002269"
     CDS             complement(7312..8613)
                     /gene="rlmD"
                     /locus_tag="ACHY09_002269"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS3885238.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8670..11426
                     /gene="barA"
                     /locus_tag="ACHY09_002270"
     CDS             8670..11426
                     /gene="barA"
                     /locus_tag="ACHY09_002270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFS3885239.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11650..12990)
                     /gene="gudD"
                     /locus_tag="ACHY09_002271"
     CDS             complement(11650..12990)
                     /gene="gudD"
                     /locus_tag="ACHY09_002271"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFS3885240.1"
                     /translation="MSSQFSTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13011..14351)
                     /locus_tag="ACHY09_002272"
     CDS             complement(13011..14351)
                     /locus_tag="ACHY09_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFS3885241.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVGLDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14353..15705)
                     /gene="gudP"
                     /locus_tag="ACHY09_002273"
     CDS             complement(14353..15705)
                     /gene="gudP"
                     /locus_tag="ACHY09_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFS3885242.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16140..16589)
                     /locus_tag="ACHY09_002274"
     CDS             complement(16140..16589)
                     /locus_tag="ACHY09_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS3885243.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     SGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(16607..17389)
                     /gene="truC"
                     /locus_tag="ACHY09_002275"
     CDS             complement(16607..17389)
                     /gene="truC"
                     /locus_tag="ACHY09_002275"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFS3885244.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDLIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMLVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17389..17718)
                     /locus_tag="ACHY09_002276"
     CDS             complement(17389..17718)
                     /locus_tag="ACHY09_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFS3885245.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDKPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18340..18885)
                     /gene="syd"
                     /locus_tag="ACHY09_002277"
     CDS             complement(18340..18885)
                     /gene="syd"
                     /locus_tag="ACHY09_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFS3885246.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18953..19801
                     /gene="queF"
                     /locus_tag="ACHY09_002278"
     CDS             18953..19801
                     /gene="queF"
                     /locus_tag="ACHY09_002278"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFS3885247.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19913..21277
                     /gene="ppnN"
                     /locus_tag="ACHY09_002279"
     CDS             19913..21277
                     /gene="ppnN"
                     /locus_tag="ACHY09_002279"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFS3885248.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5241 a         5710 c         5116 g         5308 t
ORIGIN      
        1 ctttgttgca gcccaaccaa ctattgtccc acaattacag caacaagtta cggatcttac
       61 gagtagctta aactcacaag aaaagaaaga actgacgcgg aagttagaat ctattttcaa
      121 taatacccaa gtacaaattg ctgtattaat tattcccaca accaaagacg agacaattga
      181 acaatatgct acaagggttt ttaacaattg gcgtttagga gatgccaaac gcaatgatgg
      241 gatactgatc attgttgcct ggtcggatcg cactgtccgc atccaggtag gttatgggct
      301 ggaagaaaag gtaaccgatg ctctggctgg agatatcatc cgtagcaaca tgatacccgc
      361 atttaaacaa caaaaattag ctaagggatt agagttagct ataatcgctt tgaataacca
      421 actcacttca caacaccaat attcggctaa tccttcagaa agtgaatcag cgtcttccag
      481 cgatcattac tactttgcta ttttttgggt atttgcagtg atgttcttcc ctttctggtt
      541 ttttcatcaa ggcagtaatt tttgtcgcgc atgtaaaagc agtgtctgta tttcagcaat
      601 ctatctttta gatttattcc tgttctccga taaaactttt tccattgctg tattttcctt
      661 cttttttact ttcaccatat ttatggtctt tacctgttta tgtgtacttc agaaaagagc
      721 atcaggtaga agttatcatt cagacaacag cggttccgct agaggctcag gcggcgggtt
      781 tagcggcggt ggcggttctt ctggcggtgg cggcgcatcc ggccgctggt aactcaacca
      841 taaaaaacgc cagcccgaag gctggcattt ttaaatcaga taaagtcagg cttatgcctg
      901 gcctttgatc tctttacgac cgttgtacgg tgctttttcg cccagagctt cttcgatacg
      961 aatcagctgg ttgtatttag caacacggtc agaacggctc atagaaccag ttttgatctg
     1021 gcctgcagca gtaccaacag ccaggtcagc gatagtagcg tcttcagttt cgccagaacg
     1081 gtgagagata actgcagtgt agccagcgtc tttcgccatc ttgattgcag ccagagtttc
     1141 ggtcagagaa ccgatctggt tgaatttgat caggatggag ttagcgatac ctttttcgat
     1201 accttctttc aggatcttgg tgttggttac gaacaggtcg tcaccaacca gctggatttt
     1261 gtcgcccaga actttggtct ggtatgcgaa accgtcccag tcagattcgt ccagaccgtc
     1321 ttcgatagat acgatcggat actgtttggt cagttcttcc aggaagtgag tgaattcttc
     1381 agaggtgaac gctttgttgc cttcgccagc cagaacgtat ttaccgtctt tgtagaattc
     1441 agaagctgcg cagtccatcg ccagagtgat gtctttgccc agttcataac cagcagcttt
     1501 aacagcttca gcgataacag ccagagcttc agcgttggaa cccaggttcg gcgcatagcc
     1561 accttcgtca ccaactgcag tgttcatgcc tttcgctttc agaacttttg ccaggtgatg
     1621 gaaaacttca gaacccatgc ggatggcttc tttcacagtt ttcgcgccaa ccggctgaat
     1681 catgaattcc tggatatcaa cgttgttgtc agcgtgctca ccaccgttga tgatgttcat
     1741 catcggaacc ggcatggagt atttgcccgg agtgccgttc agttcagcga tgtgctcgta
     1801 cagcggcata cctttagcag ctgcagcagc tttggcgtta gccagagata cagccaggat
     1861 tgcgttcgcg ccgaatttgg atttgttttc ggtgccgtcc aggtcgatca tgatcttgtc
     1921 aatgccagcc tgatctttag catctttgcc aatcagcgcc tgagcgatcg ggccgtttac
     1981 cgcagcaaca gctttggtta cgcctttacc caggaaacgg gatttgtcgc catcgcgcag
     2041 ttccagagct tcacgggaac cagtagaagc acctgacgga gcagctgcca taccgacgaa
     2101 accaccctcc agatgtactt cggcttcaac agtcgggtta ccacgggagt cgatgatttc
     2161 acgaccgatg atttttacga ttttggacat taggttttcc tcaagtcact agttaaactg
     2221 aaactccaga caaacaacgc gtacccaggg tacgcgttgc cgttctaact tttttactta
     2281 cttcgcctga cgtttctgga actcgctggc ggctttcaca aagcctgcaa acagcgggtg
     2341 accatcacgt ggagtagaag taaactccgg atggaactgg caagccacga accacgggtg
     2401 attcggaact tcgatgatct cgaccaactg atcatccccg gaacggcccg caacgcgcag
     2461 acctgcatct tcaatctgtt tcaacagcat gttgttgact tcgtaacggt gacgatgacg
     2521 ctcaacaatt gtcggcgcat tgtacagctg gcgaaccagg ctatcgtcaa ccaactggca
     2581 ctgctgtgcg ccgagacgca tggtaccgcc gagatcgctc ttctcgctac gaacttcaac
     2641 gttgccgttt tcatcgcgcc actcggtaat cagcgccaca actgggtact tacagtctgg
     2701 cacaaattcc gtagagttgg cgttctccat gttggcaaca tggcgagcgt aatcaattaa
     2761 cgccacctgc atacccaggc aaatacccag ataaggaata ttgttctcac gcgcaaaacg
     2821 cgcggtcgta atcatgcctt ctacgccacg atagccgaaa ccgccaggta cgaggattgc
     2881 gtccagacct ttaaggattt caacgccgcg cgtttcaaca tcttgtgaat cgatcagttt
     2941 gatgttgacg ctgacacgat tcttcagccc accgtgtttc agtgcttcga tcactgattt
     3001 ataagcatcc ggcagttcaa tgtacttgcc gaccataccg atggtgactt cacttaccgg
     3061 attcgcttct tcgaagataa cctgttccca ttcggacaga tttgcttccg ggcagtttaa
     3121 gctgaatcgt ttacaaatat aatcgtccag cccctgagat ttcaacaggc ccggaatttt
     3181 atagatggaa tcgacgtctt tcagagaaat aaccgctttt tccggaacat tacagaacaa
     3241 tgcaatcttc gcacgttcgt tcgccggaac ggcgcgatct gaacgacaaa tcaggatgtc
     3301 aggctggata ccgatggaga gcagctcttt tacagagtgc tgagtcggtt tggttttgac
     3361 ttcaccagac gctgccatgt acggcaccag cgtcaggtgc ataaacagag tgtgctcacg
     3421 gccaatttca acagccatct ggcgaatcgc ttcgaggaac ggcagggatt cgatatcacc
     3481 tacagtaccg ccgatttcga ccagtactac gtcatgacct tcgccgcctt ccagcacgcg
     3541 ctctttgatt gcgttagtga tgtgcggaat aacctgcacg gttgcgccga ggtagtcacc
     3601 gcggcgttct ttacgcagaa cgtcagagta gatacgaccc gtggtgaagt tgttgcggcg
     3661 gctcattttg gtacgaatga aacgctcgta gtgccccagg tccaggtcgg tttcagcgcc
     3721 gtcttcagta acgaacactt ccccgtgttg gattgggctc atagtacctg gatcgacgtt
     3781 gatgtacgga tccagtttca tgatggtcac attgaggcca cgggcttcaa gaatggctgc
     3841 gagggaggct gcggcaatgc ctttacccag agaggatacg accccgccgg tcacaaaaat
     3901 atagttcgtt gtcatgctga acctgagaag ttaggttgaa aagacgatgg aataaccagg
     3961 acgggaaagc agtatacccg aacatgacct gtgccacaaa ctttcattat ccctcctctt
     4021 cgccagcgca ctattgaaat caggagtgag aaaatagccc ctttggggta aatgtttttg
     4081 acgcaaatca agcgcttgtc atttaaaaaa tgacacaaat cgcgcttgac cacgtaattc
     4141 ccttagagat caatttcctg ccgttttacc tgttgccaga cttcttccat tgtttcgagg
     4201 tcaacacctg tcatttccag tccacgcgcg gcaacaatac gctccacttc gcgaaaacga
     4261 cgctcgaatt tttcgttcgc tttttgcaat gcgatttccg cttttgtccc taaatgacga
     4321 gccagattga ctgtggcaaa cagcaggtca cccatttcct cctccagttt agcctggtcg
     4381 acaacaacct gccgcgcttc gtacatcacc tcatcgatct cttcgtagac tttatcgact
     4441 accgggccaa gcgtcgtcca gtcgaagcca acgttggcgc aacgtttctg gattttttgt
     4501 gcgcgcatta aggcgggtaa actactaggg atatcgtcca gcgccgaatg ctgcgctttt
     4561 tgcgcgcgct cttcagtttt gatttgctcc caacgagcaa gaacttcgcg gctgtttccg
     4621 gcagaaccgt cagcaaaaac atgtggatga cggcgttcta atttatcgct aatcgcagcg
     4681 caaatatcat taaaatcaaa gcgcccttct tcctgggcca tttgtgcgta aaacaccacc
     4741 tggaacagca gatcgcccag ttcaccgcgc agatcgtcaa aatcttcacg ggcgatggcg
     4801 tccagcacct cgtaggtttc ttcaagggtg taaggcgcaa tggtggcaaa tgtctgctct
     4861 ttatcccacg ggcagccgtt ttccgggtca cgcaggcgct gcatgatggt gagcaaacgg
     4921 tcgatttgat tcattagaat gtcctgaaaa ttgcgggtcc gtcaggtgta ataccttcga
     4981 gtaaaagata tcgctggata cggctgacgc catatccagc tatgtaagac taactcccgt
     5041 gcaaccgacg cgcgtcgata acatccggca cctggttgag tttacccagc acgcgcccca
     5101 gcacttgcag gttgtaaatc tcaatggtca tgtcgatggt cgccagttgc tgtttggtgt
     5161 cgctacggct ggcaacgcca agtacgttca ccttctcgtt ggcgagaatg gtcgtgatat
     5221 cacgtaacaa cccactacga tcattagcca ccacgcggac caccagcgaa tatccggcgg
     5281 agtagctctc gccccatacc gcgtcaacaa tgcgttctgg cgcatgggag cgcagttccg
     5341 ccaattgttc gcaatcggcg cggtgtactg aaataccgcg cccctgggta atgaagccga
     5401 caatctcatc tccaggaatc ggctggcagc agcgcgcgat gtggtgcatc aggttaccga
     5461 ccccttctac caccacacga ccgttatctt tactgcggtt ttgcggcgtg tagctttttt
     5521 gctgaagctg tttcagcgcg gcggcgtcct gctcttcggc actcggctta ttaaattgcg
     5581 attgcaggaa gttcaccatc tgattgagac ggatatcccc gccaccaatc gccgccagca
     5641 actcgtcgac atcattgaag ttgtaacgcg gcagcagatg tttttctgct tctttcaggc
     5701 tgatccccag atgttccagc tcgtcgtcaa gaatttgccg cccagccaga atgtttttgt
     5761 cacggtcctg tttacggaac caggcgtgaa ttttcgaacg cccacggctg gttgtgacgt
     5821 aaccgaggtt tgggtttaac cagtcacggc tggggttcgg ctgtttctgg gtgataattt
     5881 caatctggtc gcccatctgc agctggtagg tgaacggcac aatgcgcccg ccaatttttg
     5941 ccccgatgca gcggtgtccg acatcactgt ggatgtggta agcgaagtcc agcggcgttg
     6001 atcccgcagg caaatcaacg acatcacctt tcggcgtaaa gacgtacact cggtcgtcaa
     6061 agacctgact acgtacttcg tcgagcattt cgccggaatc agccatctct tcctgccacg
     6121 caatcagttt acgcagccag gcaatccggt cttcatgtcc cgaacgtgcg ccgccagcag
     6181 ccgcgccctc tttatatttc cagtgcgcag caacacccaa ctctgcatct tcatgcatct
     6241 gtttggtgcg gatttggatc tcaacggttt tgccacccgg ccccagaacc acggtatgaa
     6301 tggactgata accgtttggt ttcgggttag cgacgtaatc gtcaaactca tccggcaggt
     6361 ggcgatagtg agtgtgcact atccccagtg cggcataaca atcctgtaaa cgctcggcga
     6421 caatacgtac cgcacgcaca tcaaacagct catcgaaggc gaggttcttt ttctgcattt
     6481 tgcgccagat actgtagatg tgtttcggac ggccatacac ttcagcttta acgccttcag
     6541 ctttcatctc agcgcgcaga tggccgacaa actcttcgat atagtgttcg cggtcgagac
     6601 ggcgttcatg cagcagtttt gcgatgcgtt tgtattcggt cgggtggaga taacggaagc
     6661 agtaatcttc cagctcccat ttcagttgcc caatcccaag acggttcgcc aacggcgcgt
     6721 agatattggt gcactctttt gccgccagta cgcgttcatc ttccggcgca tcttttactt
     6781 cgcgcaggtg agcaatacgc tccgccagtt tgatgaccac gcagcgaaaa tcatcgacca
     6841 tcgccaataa catccggcga acgttatcga cctgttcgga ggaaacagaa tcagtgtgcg
     6901 tcgctttcag ctggcggatc gccgccatat cacgcacgcc gtgaataagg ttaacgaccg
     6961 acttaccgac gctctcacgc agcacatctt cgctgactac gttggcatca gccagaggga
     7021 acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa tgtcgagagg atctccacca
     7081 tctcaacacc acgccacaat aacagactgg catccggatg cccctgcgtc tgttgcagac
     7141 aatacgccca ggtttcggct aagcactcac acgacttctg gctggtaata cccagacttg
     7201 cgatccattt ttccggatca aattcaccag ccttattgat atgtgcactt cttaccgcaa
     7261 ccatcgtcct ctcctttagg gaccagacct gccgaaatcg gcaaatcgta actatttaac
     7321 gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt gggaacatat ccagcatcgc
     7381 cagtcgcgca atggtatatc ctgcttttaa taacgcttcg ctatcccgag ccagcgttgc
     7441 agggttacag gatacataaa ctatacgaat aggttccagt tttataattt gctgcataac
     7501 acctgcggca cctgctcgcg ccgggtccaa caacacttta tcgaagccgt ttttcgccca
     7561 cggctgcttt gtgacatctt cttcaagatt ttcgtgataa aacgtcacat tctgtaagcc
     7621 attaagacgc gcattctgct ggcctttttc caccagcgcc ggaacacctt ctacacccac
     7681 cacactagca gcttgtgtcg ccaatggcag tgtaaagttg cccataccgc agaacagatc
     7741 cagtacgcga tcttcaggtt ccacatccag ccattccaac gcacgcgcta ccattttctg
     7801 gttcacaccc gcattgacct gaataaaatc gcgcgggcta aaagttaagc gcaacccatt
     7861 tgagtcatac cagggcatct caccagagac ggtttcgagt atctcactat cgggggcgag
     7921 atacagatcc aggccttcag aatgcgaaaa gcgttccagt ttttcgcgat cagccgaact
     7981 tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg gttgcctgta ccagttcaac
     8041 atgaccaaga tggcgtatag cttgtaagct gcccaggcat gccctgactt tgggcagcaa
     8101 tgcttcaagt tggggcacta aaatgggaca ttgtttaacg tcgacaatgt cactggagcc
     8161 cgctttgcga aaccccatct gaagttgctg tgttttcggt aagtagttca gacttaaacg
     8221 cgcgcgacgg cgatagcccc agggaacatc ggcgatcact tcagagactt cgtgtttcat
     8281 taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa tccacgctgg cgtgttgttg
     8341 ctgacagccg ccgcatacgc caaaatgagg acagcgtggc gtttcgcgtt ccgggctatc
     8401 gcttaaccgg cgcacgactt tagcgcgggc gtattgtttt ttatcttcag taacagtaac
     8461 ttccgcgttt tcctgtggca ataatccagg aataaataac gctttgccgt tatgtcgtgc
     8521 cacgccctga ccaaaagagt cgaggtcgtt gactgaaaca gttatgatct gacgcgtcgt
     8581 cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag actttctcaa tttaacagtg
     8641 tgaccttaat tgtcccataa cggaactcca tgaccaacta cagcctgcgc gcacgcatga
     8701 tgattctgat cctggcaccg accgtcctta ttggtttatt gctgagtatc tttttcgttg
     8761 tgcatcgcta taacgacttg cagcgtcaac tggaagatgc cggagccagc attattgagc
     8821 cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa tcgcgaatct atcggtcagt
     8881 taataagcgt actgcatcgt cgccattccg atattgttcg cgcgatttcg gtttatgatg
     8941 aaaataaccg actctttgtc acctccaatt ttcatcttga tccctcatca atgcagctcg
     9001 gcagcaacgt gccgtttcct cgccagctca ctgtcactcg tgacggcgat attatgatcc
     9061 tccgcacgcc gattatttct gaaagttact cccccgacga atcgcccagt agcgatgcca
     9121 aaaatagtca aaatatgctg ggatatattg cgctggagct ggatcttaaa tcggttcgct
     9181 tgcagcaata taaagagatc tttatttcca gcgtgatgat gctgttttgt atcggtattg
     9241 cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg tccgattcgc aacatggtga
     9301 ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg ggtggaagga tttatgctcg
     9361 gcgagctgga tatgctgaaa aacggtatca actcgatggc aatgtcgctg gcggcttatc
     9421 acgaagagat gcaacacaat atcgaccagg cgacgtccga tctgcgtgag acgctggagc
     9481 aaatggagat tcagaacgtt gagttggatc tggcgaaaaa gcgcgcccag gaagcggcgc
     9541 gtattaaatc cgagtttctg gcaaatatgt cacacgagct gcgtacacca ctgaatggtg
     9601 ttattggctt tacccgcctg acgctgaaaa cagaattaac accaacgcag cgcgatcacc
     9661 tgaatacgat tgaacgttcg gcaaataatt tgctggcaat tattaatgat gttctcgact
     9721 tctcgaaact ggaagccggt aagctgattc tggaaagtat tccattccca ctacgcagca
     9781 cgctggatga agtcgttacc ctgctggcac attcttctca cgataaaggg ctggaactga
     9841 cgctcaatat taaaagcgac gtgcctgata acgtgatcgg cgacccacta cgattacagc
     9901 aaatcatcac taacctggta gggaatgcaa ttaaattcac cgagaatggc aacatcgata
     9961 ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca gattgaagtg cagattcggg
    10021 ataccggcat tggtattcct gaacgcgatc aatcgcgctt attccaggcc ttcagacagg
    10081 ctgatgccag tatttcccgc cgtcatggtg gcaccgggct ggggctggtg attacacaaa
    10141 aactggttaa tgaaatgggc ggcgatattt cgttccatag cctgccgaat cgtggttcaa
    10201 ctttctggtt ccacattaat ctcgatctga acccgaacat tattatcgaa gggccatcca
    10261 cccagtgcct cgctggtaag cgcctggcct atgtcgaacc aaactccgca gcagcacaat
    10321 gcacgctgga tattttaagt gaaacgccgc tggaagtggt ttatagccca acgttctccg
    10381 cgctgcctcc cgcgcattac gacatgatgt tattaggcat agcggtgacc ttccgcgagc
    10441 cgctaacaat gcaacatgag cgattagcga aagcagtatc gatgaccgat ttcctgatgc
    10501 tggcacttcc ttgccatgca caagtcaatg ctgaaaaact taagcaagat ggtatcggcg
    10561 cgtgtctgct gaaaccatta acacctacgc gcctgttgcc tgccctgacg gaattttgtc
    10621 atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag taagctggca atgacagtca
    10681 tggcggttga tgacaacccc gctaacctga aacttatcgg cgcattgctg gaagatatgg
    10741 tgcaacatgt ggaactttgc gatagcgggc atcaggcggt tgaacgggcg aaacagatgc
    10801 cgttcgattt aatcttaatg gatattcaaa tgccggacat ggacggcatt cgggcatgtg
    10861 agctcatcca ccagctcccg catcagcagc aaacgccggt tatcgcggta acggcgcatg
    10921 cgatggccgg gcaaaaagag aaactgcttg gcgcggggat gagtgattat ctggccaaac
    10981 cgatcgaaga agagcgattg cataatttgc tgttgcgcta caagcctggc agcggtattt
    11041 cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt caacccgaat gcgaccctcg
    11101 actggcaact ggcactacgc caggcagcag gaaaaaccga tttagcgcgc gatatgctgc
    11161 aaatgttact cgatttcctg cctgaagttc gcaacaaagt tgaggaacag ctggttggag
    11221 aaaacccgga aggcctggtt gatttgattc ataaactgca tggcagttgc ggctatagcg
    11281 gtgtaccgcg tatgaagaat ctctgccaac tgatcgaaca acagctacgt agcggtacta
    11341 aagaagaaga tttagagccg gagctgctgg aactgttgga cgagatggat aatgtagcgc
    11401 gcgaagccag caaaattctc gggtaatgga cgtcttgtcg agtagcaacc ggatttttat
    11461 gacgctggtt tcagattggt gaaaagtgcc tgtgtttatg ccggatgcgg cgttaacgcc
    11521 ttatccggcc tacaaaacca attaaattca atgaattaaa aaatgatgta accctgatac
    11581 gtagcgcaac aggcagtttg gcatcagtct caaaccggct ccagatagag ccggttttgg
    11641 ttttctgtct taacgcacca tgcacgggcg cttgttatca aacgtccagc cggggatcag
    11701 gtactgcatt cccatcgcat cgtctcgcgc accaagccca tgtttctgat acagctcgtg
    11761 ggctttcatc acctgatcca tatcgatttc tacgcccaat cctggttttt gcggcacctg
    11821 taccagcccg cctttgatct caaacggttc tttggtcaga cgctgattgc cttcctgcca
    11881 gatccagtgc gtatcaatag cagtaatttt acccggtgca gcagcggcta catgagtaaa
    11941 catcgccagg gaaatatcga agtggttgtt agagtgtgaa ccccaggtca ggccaaattc
    12001 atggcacatt tgcgccacac gtaccgaacc ttgcatcgtc cagaaatgcg gatccgccag
    12061 cgggatatca acggattgta acgagagcgt atggcccatc tgccgccagt cggtggcgat
    12121 catattggtt gcggtcggca ggccagtcgc gcgacggaac tctgccatca cttcacgccc
    12181 ggagaaacct tgctccgcac cacacggatc ttctgcataa gccagcgaac ctttcaggta
    12241 tttaccaatt ttaatcgctt cgttcagcga ccaggcaccg ttaggatcga gggtaatacg
    12301 cgcctgtggg aagcgttgcg ccagtgccac aatagattct gcttcttctt ccccggccag
    12361 tacaccgccc ttcagtttga aatcgttgaa gccatatttt tcatatgccg cttccgccag
    12421 gcgcaccacc gcatccggcg tcatcgcttc ttcgtgacgc aggcgatacc agtcacattt
    12481 atcatccggc tggctttgat acggcagcgg tgtggctttg cgattaccga cgaagaacag
    12541 ataaccgagc atttcgactt cgctacgctg ttgaccatcg cccagcagcg atgccacgtt
    12601 aacccccaga tgctgcccca gcagatccag cattgccgct tctatcccgg taaccacatg
    12661 aatagtggtc cgcaggtcaa atgtctgcaa accgcgcccg ccggcatcac gatcggcaaa
    12721 tgtattacgc accagcgtca gaacgttttt gtattcaccc agcgttttac ccaccaccag
    12781 cggaatcgca tcttccagcg ttttacggat tttctcgccg ccgggaattt cccctacccc
    12841 ggtgtgacca gaattatctt tgataatcac aatattacgc gtgaagaacg gtgcgtgtgc
    12901 accactcaga ttcatcagca tactgtcatg gcccgccacc gggatgacct gcatttcagt
    12961 aacaacaggc gtcgaaaatt gagaactcat ctttatgtcc tttttcaaaa tcaatgacgg
    13021 ccgaaaacgg gacgtttacg gtcaaaggtc cagccgggga tcaggtactg catcgggccc
    13081 gcgtcgttac gcgcaccccc aggcagacgt ttataggcct catgcgcctt ttgtacctgc
    13141 tcccagtcca gtcccacgcc cagaccgggc gcatcaggaa cggcaatttt tccgttttta
    13201 atctccagcg gatttttggt caggcgacaa tcgccctcct gccaaatcca gtgggtatcg
    13261 atagcggtag gattacccgg tgccgccgca cccacatggg taaacatcgc cagagagata
    13321 tcaaagtggt tattggaatg gcagccccag gtcagccccc agtcgtcgca aagttgcgcc
    13381 acgcggactg caccggaaag cgtccagaag tgcggatcgg caagtggaat atctaccgca
    13441 ttgagcatca ccgcatgacc catttcgcgc cagttggtgg cgatcatgtt ggtcgcgacg
    13501 ggcaagccgg tcgcccgtcg aaactccgcc atcacttcac gaccggagaa accctgctca
    13561 gcgccgcacg ggtcttccgc ataggtaagg acatcattca gccctttgca cagagaaatg
    13621 gcttcatcaa gcagccaggc accattggga tcaacggtaa tccgcgcatc agggaagcgt
    13681 ttcttcaatg cacgaacagt gtcgatttct tgctcgccag gtaacacgcc acccttaagt
    13741 ttgaaatctt taaagccata gcgatcctgt gaggcttccg ccagacgcac aacggcttcg
    13801 ctgttcatcg ctttctgatg ccgcaactga taccactcat ggttgcccgg cgtattttcc
    13861 agataaggaa gatcggtttt ggtccgatca ccgatataaa acagataacc gagaacggta
    13921 atagcatcac gttgcttgcc tggccctaac agttcgcaga ccggaacatt cagcgcctga
    13981 cctaacaggt caagcaaggc ggcttccagc gccgccacgg cattaacgcg caattcaaaa
    14041 gtccaggcac ctttgccgaa ggtatcaaaa tcggctgcct gattaccttt atgcacctgc
    14101 tgaaccactt tattcaggcg cgcaacttcc tggcccagca ccatcggaat agccttgacc
    14161 agtgtttgat aaatcacctc tccgcccggc gcttcaccaa tgccggtatg cccggcatta
    14221 tcggtgagta ccacaatatt gcgggtgaaa tatgcgttat gtgcaccacc aatattaagc
    14281 aacatgctgt catgtccggc gaccggaatg actttcatat cagtaataac aggactggat
    14341 tgtgtcgtca tcttattgcc ccgcgacagg tttcagctca atacgcttga tatcgcccac
    14401 cagcaccagg tagctgagca ccgcgattaa ggcatgaaca ccaacataaa tcagcgcccc
    14461 attaaaggag ccagtcgtgc caacgatata accaattgcg attggcgtga cgatgccaga
    14521 aatgttacca aacatgttaa acaggccacc ggaaagaccg ctgatctctt ttggcgcagt
    14581 atcagccatt acagcccaac ccagcgcacc gatcccctta ccgaagaagg ccagcgccat
    14641 aaagccgatg atcatccact caacgttgac gtagttgcag aacaccatca ccatcgacaa
    14701 caacatgccc atcacgatcg gtgttttacg cgcaatgttc agtgaacccg tgcgacgcat
    14761 cagccagtcg gaaataatcc cacccagcac accgccgata aaaccgcaaa ccgccggaac
    14821 ggaagccaca aagcccgctt tcagaatcga catcccgcgc gcctgcacca gataaaccgg
    14881 gaaccaggta atgaaaaagt aagtcagggc gttgatacag tactgaccga tataaacgcc
    14941 gatcatcatc cgtgacccga gcagctgttt gatctgcccc cacttcacgc tgaacggcac
    15001 tttaactttg gtgttttgct gatccatatt gatcagcgcg ccacccgcgg cgatgtactc
    15061 cagctctttc tgatttacgc ccggatgttg atttggctcg tggatgactt tcaaccagat
    15121 aaagctgatg acaatgccca gaccgcccat aaagaagaag acgtgtgacc agcccacttc
    15181 atgagttagc cagcccataa tcggcgcgaa gataaccgtt gcgaagtatt gggcggagtt
    15241 aaaaatcgac accgccgttc ccctttcctg cgccggaaac caggccgcaa caatgcggct
    15301 gttgccaggg aaagatggcg cttcagcaag cccgaccagg aagcgcagtg taaacagggc
    15361 aacgataatg ccgaatccac taaagatatc gacgaagcct tgcagcaagg taaacatcga
    15421 ccagataaag atcgaccaga agtagacgcg ttttgaacca aaacgatcca gcaaccagcc
    15481 accagggatc tgcccgataa catacgccca tgagaaggca gagaacacat agcccattcc
    15541 cacgggatca aggccgatat ctttggccat ttccgaaccg gcaatagaaa gcgtagcgcg
    15601 gtcgccgtag ttgaaggatg tgacgataaa caacatcacc actatccagt aacgagcatt
    15661 cgtgcgtttt tccacactgc tcgcagcctg acttaaagaa ctcattgttg cactcctgaa
    15721 aattcgcgtt agccacgctc actctggact gcgacatcgc caggaaatca gaggtgacgt
    15781 agggtgtttt ttgccgtttt tataggtcgt tcgccgaata cggcgcgtgt ttatatctgg
    15841 caatagcagt ataaaaagcg cgccatagcg gctcaccgtg caacaacaca acattaatgc
    15901 gttcaatgag gcccgatttt ggcattagcc ctggacggtg gaatccactt cacggaaatg
    15961 aaaacaagaa caagaaagga agggttaaaa cgaagaaata aagaagagta tgaaatggat
    16021 cgcttgactc caggcaaacg ccagtaaaaa tccgcgctat gaagcagttt ttactggcat
    16081 ttgcctgaaa agattcgatt cagcaccgct aaaacggcat ttaccgctcg ctgaacatat
    16141 caggacaaca tcgtgcccca atgttcgacc cacggatttg attccgtttc cggttccggg
    16201 ttctcactgg cgtcaatcaa cagcatttcg ccaacccgct gagcgctctg ttcctgcaac
    16261 aaggcatcga actgtttgcc actattgcag aaattcacat aactactgtc gccgagcgca
    16321 atcacgccat aacgcagatt cggctggaag cccagggtat ctttaattcc ctgaaagagc
    16381 ggcacaatgc tatcaggaag gtcgccctgc ccggtcgtgg acgtaaccac cagaacatac
    16441 ttatcctgat agggcagcca gtcgcttaat tcaggatctt caaataccgt tgctttgtgg
    16501 ccctgcgcgg tcagaattgc ttccgcttct tccgccacta acagcgaatt tccgtacata
    16561 gtgccgacaa aaataccaat ttccgccata ccgttttctc cctggattag gaacttatct
    16621 ctccatcctg acccgatggc gcactgaact caaccctttc attttcagga agcagacccc
    16681 gccagccaaa ttgtgataac gcctgcatcc aggtgtcgtc caggcccgcg tgaatagtca
    16741 gtggctcgcc agtaaaagga tgcgtcaatg acaactgact ggcatgcagc attaaccgct
    16801 gaaggccaaa atgttcagca ccgctgcgat tctggcgtaa atcaccatgt ttgctgtcgc
    16861 caataatcgg gtggcgcaaa tgggcaagat ggcgacggag ctggtgctta cgtccggttt
    16921 tcggctccag ttccaccagg ccgtagcgcg tggtcgggta acgtccggtc gctaccagca
    16981 tttctacggt cgccagaccg cgataatgcg tcactgctgg ctgcgggcct ttatcttcgc
    17041 gggcaaattt atcagcgatt ttgtccagtt cttccaccag tggataatcc agcaccgctt
    17101 cttccatcaa ccagccgcgc acaatcgcat ggtaacgttt ctggatttgg tgctgttcaa
    17161 actgttgtgc cagcagccgt ccggcctcgc tggataatcc catcaacaac acaccagaag
    17221 tgggtcggtc cagacgatga gcagtaaaca catgctggcc tatcaggtca cgcacggttt
    17281 gcatgaccac tactttctcg tcgcgatcca gccagctgcg gtgaaccagc cagccggagg
    17341 gtttatttac cgcaaccagc cattcatcct gatagagtat ttccagcatt agctcgcatc
    17401 atccgcaaaa agagcatcca gtttttccag ctcagccaga ataagcgcgc gttgcggatg
    17461 atcagtcgcc agcgccattt cataataggg tgcaacggca aaagcgcccg gtaacggctg
    17521 gttgttatct aacaaatcgt gcattcgcgg gatcagcacc cactgcaacc actccagtgg
    17581 ttccatggtg tccataaaga acggttgggt actgttaaat tgatgcggct ggggtttatc
    17641 gtttcgccag tgctgatgtt cacgcagtaa cgcttcaagc gcctgcaact ggagacgaac
    17701 gcggtcatga gtggtcataa agcaacctca ataagaaaaa ctgccgcgaa ggatagcagg
    17761 aataaaaaaa gggagcactg tattcacagt gctcccggtt cgtttcgcag cattccggct
    17821 acttttgttg ctccctgctc atccttgaca acttttcctc tggccttgcg gccaatcgtt
    17881 catcctgaac tattgcttcc tgctcacacc accccgatgt gatacttcat cctgaagtgt
    17941 tcctggcctt cctgacccac cgaatcatcc tgaccggttc tcattctcca tcctggaggt
    18001 gtcctttaac gcgtcctgcg tcatcctctt cgcttcatcc agaagccttt ccctgaaaca
    18061 ccatcctggt gtgtccttca gaagtgtcat catcctgatg ttcacttcgt tgtctgactc
    18121 cctgtcgacg aagatagaat cgtctttttc gcgaagtctt acaaggcgct tacagacaat
    18181 gccttaagga aaatttcata tgaaagttaa gaattaacaa gcaacatgtt gtttttaaat
    18241 gagaataacg attcttatct ttttaagtat tcgcttattt cctggcgatc tcctacagag
    18301 tctgtaagag atctctcaca accagatggg gtatttggat tacagaagag gcttaagttg
    18361 attaaggaat tccgcaagat ttgaggctaa atgcgtccgt ttacgtgttc ctagcgtctc
    18421 tttgcacact tcaccggaga gattacacac cgagatgacc tctaactctt cttccagcgt
    18481 cgcgataaat agcgtgggcg gcagttttag ccttttctga gtcaccagat gaccaatcag
    18541 attttcctgc acccgacgga aatcgtcttc actccaggtt tgcagcaatg tcagtttgat
    18601 atcgccaaac tgcgcgtgca tatccccggc aaactgagtc gtataaaagg tatgaatcgt
    18661 gggttgtatc acaatatcaa aagcgcgttc aaccgcgttt acattttgtt ccccggtaaa
    18721 cggctggggt tgccagtata cggcatcttc ggtagttgaa ataatgcacg gtgaaggaac
    18781 gccgtacagt tcctcactta acggccagct tttatgctct tcatgccacg catcgcagta
    18841 gcgtgcagta aaatctttca gggcttgtgc ggtcaaatcg tccactaatt tctctcttca
    18901 cttaaaccag atacacttgt cgtttagttt atctggttta tgacggtgaa acatgtcttc
    18961 ttatgcaaac catcaggcac ttgcgggcct gactcttgga aaatcaaccg attaccggga
    19021 tacctatgac gccagcctac tgcaaggcgt tccacgcagc ctgaatcgcg acccgctggg
    19081 tctgaaagcg gataacctgc cttttcacgg tacggatatc tggacgctgt atgaactttc
    19141 ctggctgaat gcgaaaggtt tgccgcaggt cgctgtcggt catgttgaac ttgattacac
    19201 cagcgcaaat ctgattgagt cgaagagttt taagctctat ctcaacagtt ttaaccagac
    19261 gcgttttaat aactgggatg aggtgcgcca gacgctggag cgcgacttaa gcacttgcgc
    19321 tcagggtaag gttagcgtgg cgttatatcg tcttgatgaa ctggaaggcc aaccgatagg
    19381 tcatttcaat ggcacttgca ttgatgacca ggatatcact atcgataact atgaattcac
    19441 tactgactat ctggagaatg ccaccagtgg cgaaaaagta gtagaagaga cgctcgtcag
    19501 ccacctgctg aaatcaaatt gcctgatcac ccatcaacca gattggggat cgatccaaat
    19561 tcagtatcgt ggacgccaaa ttgacagaga aaaactgctg cgttacctgg tttctttccg
    19621 tcatcacaac gagttccacg aacagtgcgt ggaacgcatc tttaatgacc tgttacgctt
    19681 ctgccagcca gagaaattga gcgtttacgc acgttatacc cgtcgtggcg gtctggacat
    19741 taacccgtgg cgcagtaata gcgattttgt cccatcgacc acaagactgg ttcggcaata
    19801 aattttttct caattttgcg tgctggattc acgcagaagg ttgtgaaagg tcatcaggca
    19861 gggctattgt aatcaaaggg aatgacgata ttcgtcccat aaggagtttt tcttgattac
    19921 acatattagc ccgcttggct ccatggatat gttgtcgcag ctggaagtgg atatgcttaa
    19981 acgcaccgcc agcagcgacc tctatcaact gtttcgcaac tgttcacttg ccgtactgaa
    20041 ctccggtagt ttgaccgata acagcaaaga attgctgtct cgttttgaaa atttcgatat
    20101 taacgtcctg cgccgtgaac gcggcgtaaa gctggaactg ataaatcccc cggaagaggc
    20161 ttttgtcgat gggcgaatta ttcgcgcttt gcaggccaac ttgttcgcgg ttctgcgtga
    20221 cattctcttc gtttacgggc aaatccataa caccgttcgt tttcccaacc tgaatctcga
    20281 caactccgtc cacatcacta acctggtctt ttccatcttg cgtaacgctc gcgcactgca
    20341 tgtcggtgaa gcgccaaata tggtggtctg ctggggcggg cactcaatta acgaaaacga
    20401 gtatttgtat gcccgtcgcg tcgggaatca gctgggcctg cgtgagctga atatctgcac
    20461 cggctgtggt ccgggagcga tggaagcgcc gatgaaaggc gctgcggtcg gacacgcaca
    20521 gcagcgttac aaagacagtc gttttattgg tatgacagag ccgtcgatta tcgccgctga
    20581 accgcctaac ccactggtca acgaattgat catcatgccg gatatcgaaa aacgtctgga
    20641 agcgtttgtc cgtatcgctc acggtatcat tatcttccct ggcggtgtgg gtacggcaga
    20701 agagttgctg tatttgctgg gaattttaat gaacccggcc aacaaagatc aggttttacc
    20761 attgatcctc accggcccga aagagagcgc cgactacttc cgcgtactgg acgagtttgt
    20821 cgtgcataca ttgggtgaaa acgcgcgccg ccattaccgc atcatcattg atgacgccgc
    20881 tgaagtcgct cgtcagatga aaaaatcgat gccgctggtg aaagaaaatc gccgcgacac
    20941 aggcgatgcc tacagcttta actggtcaat gcgcattgcg ccagatttgc aaatgccgtt
    21001 tgagccgtct cacgagaata tggctaatct gaagctttac ccggatcaac ccgttgaagt
    21061 gctggctgcc gacctgcgcc gtgcgttctc cggtattgtg gcgggtaacg taaaagaagt
    21121 cggtattcgc gccattgaag agtttggtcc ttataaaatc aacggcgata aagagattat
    21181 gcgtcgtatg gatgacctgc tacagggttt tgttgcccag catcgtatga agttgccagg
    21241 ctcagcctac atcccttgct acgaaatctg cacgtaatct ccctcccccg gtgacgtttg
    21301 ttgccgggtt ttctcgtttt tggtcacata cccatcaatt tatttcattt gttatatgaa
    21361 tgtttcttac caccc
//
LOCUS       DAZUCC010000071        21123 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZUCC010000071 DAZUCC010000000
VERSION     DAZUCC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.071"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..93)
                     /gene="ylcJ"
                     /locus_tag="ACHY09_002280"
     CDS             complement(<1..93)
                     /gene="ylcJ"
                     /locus_tag="ACHY09_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
                     /protein_id="HFS3885249.1"
                     /translation="MSLVLCFLLMSLFFMYSFVLSRLWRKKIAIR"
     gene            complement(141..473)
                     /gene="emrE"
                     /locus_tag="ACHY09_002281"
     CDS             complement(141..473)
                     /gene="emrE"
                     /locus_tag="ACHY09_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFS3885250.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(541..843)
                     /locus_tag="ACHY09_002282"
     CDS             complement(541..843)
                     /locus_tag="ACHY09_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFS3885251.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(840..1541)
                     /locus_tag="ACHY09_002283"
     CDS             complement(840..1541)
                     /locus_tag="ACHY09_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFS3885252.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1538..2557)
                     /locus_tag="ACHY09_002284"
     CDS             complement(1538..2557)
                     /locus_tag="ACHY09_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS3885253.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(2554..3093)
                     /locus_tag="ACHY09_002285"
     CDS             complement(2554..3093)
                     /locus_tag="ACHY09_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFS3885254.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(3163..3393)
                     /locus_tag="ACHY09_002286"
     CDS             complement(3163..3393)
                     /locus_tag="ACHY09_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3885255.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            3498..4187
                     /locus_tag="ACHY09_002287"
     CDS             3498..4187
                     /locus_tag="ACHY09_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFS3885256.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4783..4989
                     /locus_tag="ACHY09_002288"
     CDS             4783..4989
                     /locus_tag="ACHY09_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFS3885257.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            5065..5361
                     /locus_tag="ACHY09_002289"
     CDS             5065..5361
                     /locus_tag="ACHY09_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFS3885258.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            5367..6152
                     /gene="bet"
                     /locus_tag="ACHY09_002290"
     CDS             5367..6152
                     /gene="bet"
                     /locus_tag="ACHY09_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFS3885259.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIHASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            6149..6829
                     /locus_tag="ACHY09_002291"
     CDS             6149..6829
                     /locus_tag="ACHY09_002291"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFS3885260.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            6826..7008
                     /locus_tag="ACHY09_002292"
     CDS             6826..7008
                     /locus_tag="ACHY09_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFS3885261.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            6981..7172
                     /locus_tag="ACHY09_002293"
     CDS             6981..7172
                     /locus_tag="ACHY09_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFS3885262.1"
                     /translation="MHKASPVELRTSIEVAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            7249..7464
                     /locus_tag="ACHY09_002294"
     CDS             7249..7464
                     /locus_tag="ACHY09_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS3885263.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            7563..7772
                     /locus_tag="ACHY09_002295"
     CDS             7563..7772
                     /locus_tag="ACHY09_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFS3885264.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQ"
     gene            7829..8107
                     /locus_tag="ACHY09_002296"
     CDS             7829..8107
                     /locus_tag="ACHY09_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS3885265.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            8079..8144
                     /locus_tag="ACHY09_002297"
                     /pseudo
     CDS             8079..8144
                     /locus_tag="ACHY09_002297"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8306..9469
                     /gene="intD"
                     /locus_tag="ACHY09_002298"
     CDS             8306..9469
                     /gene="intD"
                     /locus_tag="ACHY09_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFS3885266.1"
                     /translation="MSLFRRNEIWYASYSLPGEKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(9485..9561)
                     /locus_tag="ACHY09_002299"
     tRNA            complement(9485..9561)
                     /locus_tag="ACHY09_002299"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9525..9527),aa:Arg,seq:tct)
     gene            9832..10698
                     /gene="folD"
                     /locus_tag="ACHY09_002300"
     CDS             9832..10698
                     /gene="folD"
                     /locus_tag="ACHY09_002300"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFS3885267.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            10700..10912
                     /gene="ybcJ"
                     /locus_tag="ACHY09_002301"
     CDS             10700..10912
                     /gene="ybcJ"
                     /locus_tag="ACHY09_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFS3885268.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            11020..11541
                     /gene="ybcI"
                     /locus_tag="ACHY09_002302"
     CDS             11020..11541
                     /gene="ybcI"
                     /locus_tag="ACHY09_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS3885269.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHKVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(11577..12962)
                     /gene="cysS"
                     /locus_tag="ACHY09_002303"
     CDS             complement(11577..12962)
                     /gene="cysS"
                     /locus_tag="ACHY09_002303"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS3885270.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            13136..13630
                     /gene="ppiB"
                     /locus_tag="ACHY09_002304"
     CDS             13136..13630
                     /gene="ppiB"
                     /locus_tag="ACHY09_002304"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFS3885271.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            13633..14355
                     /gene="lpxH"
                     /locus_tag="ACHY09_002305"
     CDS             13633..14355
                     /gene="lpxH"
                     /locus_tag="ACHY09_002305"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFS3885272.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            14473..14982
                     /gene="purE"
                     /locus_tag="ACHY09_002306"
     CDS             14473..14982
                     /gene="purE"
                     /locus_tag="ACHY09_002306"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS3885273.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            14979..16046
                     /gene="purK"
                     /locus_tag="ACHY09_002307"
     CDS             14979..16046
                     /gene="purK"
                     /locus_tag="ACHY09_002307"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS3885274.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPVQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(16379..17272)
                     /gene="ybcF"
                     /locus_tag="ACHY09_002308"
     CDS             complement(16379..17272)
                     /gene="ybcF"
                     /locus_tag="ACHY09_002308"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS3885275.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTKDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(17269..18084)
                     /gene="ylbF"
                     /locus_tag="ACHY09_002309"
     CDS             complement(17269..18084)
                     /gene="ylbF"
                     /locus_tag="ACHY09_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFS3885276.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVMAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFIHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKS"
     gene            complement(18095..19354)
                     /gene="ylbE"
                     /locus_tag="ACHY09_002310"
     CDS             complement(18095..19354)
                     /gene="ylbE"
                     /locus_tag="ACHY09_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFS3885277.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLDQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPHIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIFLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(19364..21031)
                     /gene="fdrA"
                     /locus_tag="ACHY09_002311"
     CDS             complement(19364..21031)
                     /gene="fdrA"
                     /locus_tag="ACHY09_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFS3885278.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSASSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVIPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQPHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLVRLLERLQ"
BASE COUNT         5176 a         5617 c         5356 g         4974 t
ORIGIN      
        1 tcgtattgca attttcttac gccataatct tgaaagcaca aaagaataca taaaaaataa
       61 agacattaac aaaaagcata aaacgaggct catataaata taagagcctc catattttag
      121 tcgtttagaa acaaattatt ttaatgtggt gtgcttcgtg acaataaatt aataaccaac
      181 acaccggcac aaatcaacat catgcctata atggctggca ggtccagccg ttggccgaaa
      241 aatccccatg acagtaagct aatcaggaca ataccgactc ctgaccagat agcataagca
      301 atccctgtag gaatataagc cagcgtctga gctaataacc agaatgatgc acaataacaa
      361 ataattgtac caacagatgg ccataaccgt gtaaaacctt ctgaaaactt cattaaggtt
      421 gtaccaatga cctctgcaag tattgcacca ccaagataaa tataagggtt catagcatat
      481 tctttcctgt tcaaactgga gagaattgta ctacagtttg aactcaactc acctgtttca
      541 tcattgtgtt cccattgatg ttcttttata taccctcagt acccgtttca tcgcggcact
      601 ctggcgacac tccttaaaaa tcagattcgt gctcaccttt ccttcccgtt cttctctggt
      661 agcgaaccgg taatacaccg ttcgccagac cttaccatca acgaccagga ttcctgcccg
      721 cgccatttta gccgcagcct gatttatgct ggttacggtt gcgcctgtta ccgcggcaac
      781 gtcctgtgca cagaagctct tatgcgtccc caggtaatga ataattgcct ctttgcccgt
      841 catacacttg ctcctttcag tccgaactta gctttgattt ctgcgatctt cgccagagcc
      901 tgtgcacgat ttagaggtct accgcccatg acaggaagtt gttttactgg ttcaggtatc
      961 gcctcaccac ggttaattcg cgcggtcata caggacagtt catcggcagc cttgcgccgt
     1021 aattccgcgt cagtcaacgc attggcccgc atgttctgat acaggttggt aaccagccag
     1081 tagtgcgcgt ttgatttcca cggataagac tctgcatccg gatacagacc acgcttccgg
     1141 caatactcgt aaaccatatc aaccagctcg ctgacgtttg gcagcccggc ggtaacggat
     1201 gcttcttccc ggcaccatgc aacaaactgc ccgggtgatg gcagaaatgg tctattctgc
     1261 cgacgggcaa cacgcattcc ggcgctcacc tgttccatcg tggtgatccc gttttcccgg
     1321 aaagccagaa cccactggcg gcggatttcg ttcagttcgt tctggtcacg attagccagg
     1381 ctcgccggga aagttgccag taactggctg aatacaccgt tgattatctg cgctacctgc
     1441 tgtacctgcg gcttttcgtc gtactgttcc ggcatgttgt tggcgatccg acgcatctgc
     1501 tcacggtcaa agttaatcat ctgtgcggcg atgtttttca tagatccacc ccgtaaatcc
     1561 agtctgtgtt tgtcaggtcg agttttggtt tgctagctgt cacgcctgcc tgttgcttgt
     1621 tacggttgat ttcgagttgg gtccacttgt cgcggagttt ggccggactt agcacgttac
     1681 cggaccagaa gttgtcctgg catgcccagc ggaacagcac gcacatgtcg cggtggttac
     1741 gtccgtcacg ttcacgcatc aggcggatat cgttagccca ccctgcaaaa ttcggttttc
     1801 tggctgatgg cgcgatggtc ttcaccatgt caaacatcca ctctgcggcg gtcaggtctt
     1861 ctgctgtccc ccacttgctg ccgctctgaa ttgcagcatc cggtttcacc acaggaagat
     1921 cgttttctgg ttggtcagag gattcgccag aattctcgga cgaaaaaggt tttatattgt
     1981 cttttgttag tttgtctttt gtgtttacct gattcgggta aacgccttta cctgatttgg
     2041 gtaaactttt tttacctgat tcaggtaaat ttacctcttt caggtaaact ttatttttct
     2101 tacctgattc gggtaatgtt gaccattcac tgaccacatt attaatgccg gtattccgcc
     2161 cgctctgaat aaaaatccca cgctttacca gaacactttt tgcagcagaa cacttgtgcg
     2221 gcaatatccc ggttaattcg gaaagttgct cgttgctaac ccaatccagt tttttattaa
     2281 agccatatgt tttgcgcatg acagccagaa agaccagaag ctggtgctgt gttaatccgg
     2341 ccagcatcac agcttccagc aactcatttg caatgcgcgt ataaccatca tcgagatctg
     2401 ccacgcgcgg ctccttttgt gccgcatccg gcactggaaa attgaatatc tcagcagtgt
     2461 ttgccataat tcctcccgca atgagtgtgt tacgatttgc acctgaaagt cggttctgtt
     2521 ccagcagacc ggctttcgcc atttctgaac ctgtcatatc gcccccagca tggtagtaac
     2581 catcgccatc aatggaccag ccagatctgg gtccacacga aacatcgaca caataccttc
     2641 actaatttcc ttcagtttct ggtggcgtgg tgcgttgaga atgacagcct gttttgcctc
     2701 actgagttcc ttttccattt cagccaacct agccatgaag ctatcctgct caaccaggta
     2761 accgcgatat tccagcggta gtaccgccag aattgccggg gtcagttcac gcacgttatt
     2821 tcggtatttt tcagaatcga atttgttatc gaggaagcgg aacagcttct ggcgtgcacg
     2881 gctgacatca tcagggaaat cgatggtgcc gccgccctgc tcccgatact cattcacaat
     2941 gagtgtggca acgacatcct gattatctac agccgaccag gcgcggacgg catcacggat
     3001 tttttcgtgg cctggcacct gttttgtttg agaacgattt atcaccgcag tcgggctaaa
     3061 tccgctagtc tgttggtatg taagtggttg cataattgac tcctttagtt tgaattgact
     3121 gttaagttga ttgcttattg ttaaagagcg tgaaatggaa atttaagctg cgttcttttc
     3181 ggtgtgtgga aacaacttcg gaagatccgg gcgaatctgg tatgccttca ctactccacc
     3241 agtagccgta acaatgctgc cgacatgttc aggggatacc tttgctttgt tgtgaagcca
     3301 cttatagacg gcctgctgtg aaacttcgca agcagcgccc agtttctttt gtgaaccaac
     3361 gatattgatc gctgttttga tagctgggtt cataacaacc tccgtggtta atttgaatca
     3421 agattaaaac tatggttgtt tttagtcaac aaccattttc gtttgatgga ataaaacctt
     3481 ggttgtacat ttggactatg aaaacaacac tctcagaaag acttaaagaa gccagattag
     3541 cgcgaggcct tacacaaaag gcgcttgggg atttggtcgg ggttagccag gctgctattc
     3601 agaaaatcga aacagggaaa gctaatcaaa caactaaaat cgtggagatc gcgaacgctt
     3661 tgggtgtgcg cgcagaatgg ttatcttctg gcgttggaaa tatgtcagac agtacagtgc
     3721 aaccaataca atcaactgtc agccattcca aatacttcaa gattgacgtt cttgatatag
     3781 aagtcagtgc tgggccggga gtcatcaacc gtgagtttgt agaagttcta cgctcggttg
     3841 agtactcgtt tgacgatgct cgtcacatgt tcgatggtag gaaggcggaa aatatccgca
     3901 tcattaacgt gcgtggtgac agcatgtcag gaacgatcga accaggtgat ctgctgttcg
     3961 ttgatatcac agttaaatct ttcgacggtg atggtatcta tgcgtttctg tacgacgaca
     4021 cagcccatgt aaagcgcctg caaatgatga aggataagct gctggtcatc tctgataaca
     4081 aaagctactc accgtgggac ccgatcgaga aagacgagat gaaccgggtg ttcatcttcg
     4141 gtaaggttat tgggagcatg ccgcagacat ataggaagca tgggtagtac caattaaaaa
     4201 ttatcaactg ggcattgtgc tcattcagta aagaactaat tcctatcttt gctctaggta
     4261 gtaatattaa gccaccgcaa taatatcttt acctaacggc gtaagaatcc cggtcaccgt
     4321 gccgggtttt cttttgccct cccctcatca cacacaccgt taaaaaaacc accataacct
     4381 cgcttcagtt atcgctatgc gattcaagtc acaaaataaa tccatcctaa atacaaccag
     4441 ttatatctaa aacaaccaat aaaacaactt ttgttgttga cgataaaaca actatagttt
     4501 taaataagtt catcgcaaca acacaacgat acggcaacta cctgattcac cgttgcgatg
     4561 accgcttaga tccgcagttt gaatttcagc aggcttcggg gagtgcgagg ggtgaaacgg
     4621 acgcgtgaac gtcggtgtga ccagctgaaa ttaactcaac atttcatacc ttagtcgctt
     4681 caacgaggcg gcttagttat gacaaccggc ggccatccac cgcctgaata cgcgcagaag
     4741 tctctatatg ttcagcagcc cagcttacgg gcaggagttt ttatggttca tcaacattat
     4801 ggaacgcaga ccgttaatcg aggtgcggtc atgccaggaa tgctggtcaa acacaaagat
     4861 ggtacctgga ctgcatcagc taatttacgc ggacggcttt atctgcatcg cggcatcgag
     4921 cgcacttata cccgtgattt gctcgtggaa gtttttctcg acggacgcgg taacggcctg
     4981 aatcactaat cccctttcct gttttcctaa tcagcctggc atttcgcggg cgatattttc
     5041 acagccattt tcaggaggtc agccatgaac gcttattaca ttcaggatcg tcttgaggct
     5101 cagagctggg cgcgtcacta ccagcagatc gcccgtgaag agaaagaggc agaactcgca
     5161 gacgacatgg aaaaaggcct gccccagcac ctgtttgaat cgctatgcat cgatcatttg
     5221 caacgccacg gggccagcaa aaaagccatt acccgtgcgt ttgatgacga tgttgagttt
     5281 caggagcgca tggcagaaca catccagtac atggttgaaa ccattgctca ccaccaggtt
     5341 gatattgatt cagaggtata aaacggatga gtacagcact cgcaacgctg gcagggaagc
     5401 tggctgaacg tgtcggcatg gattctgtcg acccacagga actgatcacc actcttcgcc
     5461 agacggcatt taaaggtgat gccagcgatg cgcagttcat cgcattgctg atcgtcgcca
     5521 accagtacgg ccttaatccg tggacgaaag aaatttacgc cttccctgat aagcagaacg
     5581 gcattgttcc ggtggtgggc gttgatggct ggtcccgcat catcaatgaa aaccagcagt
     5641 ttgatggcat ggactttgag caggacaatg aatcctgtac atgccggatt taccgcaagg
     5701 accgtaatca tccgatctgc gttaccgaat ggatggatga atgccgccgc gaaccattca
     5761 aaacccgcga aggcagagaa atcacggggc cgtggcagtc gcatcccaaa cggatgttac
     5821 ggcataaagc catgattcag tgtgcccgtc tggccttcgg atttgctggt atctatgaca
     5881 aggatgaagc cgagcgcatt gtcgaaaata ctgcatacac tgcagaacgt cagccagaac
     5941 gcgacatcac tccggttaac gatgaaacca tgcaggagat taacactctg ctgatcgccc
     6001 tggataaaac atgggatgac gacttattgc cgctctgttc ccagatattt cgccgcgaca
     6061 ttcacgcatc gtcagaactg acacaggccg aagcagtgaa agctcttgga ttcctgaaac
     6121 agaaagccac tgagcagaag gtggcagcat gataccggac attatcctgc agcgtaccgg
     6181 gatcgacgtg agagctgtcg aacaggggga tgatgcatgg cacaaattac ggctcggcgt
     6241 catcaccgct tcagaagttc acaacgtgat agcaaagccc cgctcaggaa agaagtggcc
     6301 tgacatgaaa atgtcctact tccacaccct gctggctgag gtttgcaccg gtgtggctcc
     6361 ggaagttaat gctaaggcgc tggcctgggg aaaacagtac gagaacgacg ccagaaccct
     6421 gtttgaattc acttccggcg tgaatattac tgaatccccg atcatctatc gcgacgaaag
     6481 tatgcgcacc gcctgctccc ccgatggttt atgcagtgac ggcaacggcc ttgaactgaa
     6541 atgcccgttt acctcccggg atttcatgaa attccggctc ggtggtttcg aggcaataaa
     6601 atcggcttac atggcccagg tgcagtacag catgtgggtg acgcgaaaag atgcctggta
     6661 ctttgccaac tatgacccgc gcatgaagcg tgaaggcctg cattatgtcg tgattgagcg
     6721 gaatgaaaag tacatggcga gttttgacga gatggtgccg gagttcatcg aaaaaatgga
     6781 cgaggcactg gctgaaattg gttttgtatt tggggagcaa tggcgatgac gcatcctcac
     6841 gataatatcc gggtaggcgc gatcactttc gtctactccg ttacaaagcg aggctgggta
     6901 tttcccggcc tttctgttat cagaaatcca ctgaaagcac agcggctggc tgagaagata
     6961 aataataaac gggaggcggt atgcacaaag catctcctgt tgagttaaga acgagtattg
     7021 aggtggcaca tagccttgct caaattggag tcaggtttgt gccaatacca gtagaaacag
     7081 acgaagaatt tcatacgtta gccacatccc tttcacaaaa gctggaaatg atggtggcga
     7141 aagcagaagc agatgagaga gaccaggtat gacaaccact gaatgcattt ttctggcagc
     7201 gggcttcata ttctgtgtgc ttatgcttgc cgacatgggg cttgttcaat gacacctcag
     7261 caagaaaacg cccttcgcag cattgcccgt caggctaatt ctgaaatcaa aaaagccaga
     7321 cagcattttc cggataaaaa cgtcgatgac atttgccgta gcgtactaaa gaagcaccgc
     7381 gaaacggtaa cgctgatggg attcacaccg actcatttaa gcctggcgat cggcatgttg
     7441 aacggcgtct ttaaggaacg gtaaacatga aaagcaaaat catcagggag ctacaggctc
     7501 cttttttatt attcgcattt accctcaagc gtattaacca acaattcagg gattaatgaa
     7561 agatggcaga catcattgat tcagcatcag aaatcgaaga attacagcgc aatacagcaa
     7621 taaaaatgcg tcgtctgaac taccagactg tatccgcaac tcattgttgt gagtgtggcg
     7681 atccgataga tgagcgaaga cgcctggctg ttcagggttg tcggacttgt gcgagttgcc
     7741 aggaggagat cgaacttaag aacaaacaat agggactgtg atggcctcaa agcagcaaat
     7801 ttcaacatcg tccaactgag gtgtaaaaat gttcagaatc atttttccta acacctggta
     7861 cgtcgaccac cacggcactc cctgcaaaat cctgcgttct acccacaaca aagttcacta
     7921 catccgaaaa ggcagaacat gtatcgccag catgttccgc tttaatcatg actttgaacc
     7981 tgtgaataaa gctgatgcag atcggatagc agaagagatc gaaacggcag aacacattaa
     8041 gaagttacgt gacatgcgtt caaaaagcag aggtaaccat ggaatcatac agcctcacac
     8101 tcgatgaggc ctgtcagttt cttaagatat ccagaccaac cgccaccaac tggatacgaa
     8161 caggccgcct acaggcaaca cgtaaagatc caaccaagcc aaaatctcct tacctcacaa
     8221 cacggcaagc ctgcattgcg gcgcttcagt ctccgctgca tactgtccag gtgagcgcgg
     8281 gtgatggcat aacagaggaa agaaaatgtc actcttccgc agaaatgaaa tatggtatgc
     8341 ctcgtattcg ctcccgggcg agaaacgaat taaggaatct cttggcacaa aggacaagcg
     8401 gcaagctcag gagttgcacg acaagcgaaa agcagaactc tggcgagtag aaaagctagg
     8461 ggatttacct gatgtcactt ttgaagaggc ctgcctaaga tggcttgagg aaaaagctga
     8521 taaaaaatct ctcgattcag ataaaagccg gattgagttc tggcttgaac attttgaggg
     8581 tataaggctt aaagatatct cggaggcaaa gatttactct gctgtaagca gaatgcataa
     8641 cagaaagacg aaagaaatat ggaaacagaa agttcaggcc gccatcagga aaggtaaaga
     8701 accgcctgtt tatgaaccaa agccagtatc aactcagaca aaggcaaagc atcttgccat
     8761 gataaaggcc attctccgtg ctgcagaacg cgactggaag tggctggaaa aagcgcctgt
     8821 catcaagata ccagcggtca gaaacaagcg agtcagatgg ctggaaaagg aggaagcaaa
     8881 acgccttatt gatgagtgcc ccgaaccact gaaatctgtc gtcaagtttg cgctggcaac
     8941 tggtctgaga aagtcgaaca tcataaatct ggaatggcaa caaatcgaca tgcagcgacg
     9001 agttgcctgg gtgaatccag aagagagcaa atcaaaccgc gccattggtg tggcgctgaa
     9061 cgataccgcc tgtaaagtgt tgcgtgatca aataggcaag catcacaaat gggtgtttgt
     9121 acataccaag gcggctaagc gagcagatgg aacatcaacg cctgcggtca ggaagatgcg
     9181 catcgacagc aagacatcat ggctatcagc ttgtcgtcgt gcaggaattg aagatttccg
     9241 tttccatgac ctcagacaca cctgggcaag ctggctgatt cagtcaggcg tcccattatc
     9301 agtgcttcag gaaatgggcg gatgggagtc catagaaatg gttcgtaggt atgctcacct
     9361 tgcgcctaat catttgacag agcatgcgag gaaaatagac gacatttttg gtgataatgt
     9421 cccaaatatg tcccactctg gaattatgga ggatataaag aaggcgtaac tgattgaatt
     9481 gtaatggcgc gccctgcagg attcgaacct gcggcccacg acttagaagg tcgttgctct
     9541 atccaactga gctaagggcg cattgatacc acggtgtgct gtaatcgcgt gaattatacg
     9601 gtcaaccctt gctgagtcaa tggcttttga accagttgct gaacaagtgt acgaccgcat
     9661 cagatgtagc tcactatggc ggcaaaccaa cgacggttat catgataggt gtgaatcagc
     9721 caggtacaga aggttttact tttcagaaaa cgaaaactga cagcgcgcca cgcttctgac
     9781 aaaatagacg catccccttc gacctacgta acagatggaa tcctctctct gatggcagca
     9841 aagattattg acggtaaaac gattgcgcag caggtgcgct ctgaagttgc tcaaaaagtt
     9901 caggcgcgta ttgcagccgg actgcgggca ccaggactgg ccgttgtgct ggtgggtagt
     9961 aaccctgcat cgcaaattta tgtcgcaagc aaacgcaagg cttgtgaaga agtcgggttc
    10021 gtctcccgct cttatgacct cccggaaacc accagcgaag cggagctgct ggagcttatc
    10081 gatgcgctga atgccgacaa caccatcgat ggcattctgg ttcaactgcc gttaccggcg
    10141 ggtattgata acgtcaaagt gctggaacgt atacacccgg acaaagacgt ggacggtttc
    10201 catccttata acgtcggtcg cctgtgccag cgcgcgccgc gtctgcgtcc ctgcaccccg
    10261 cgcggtattg tcacgctgct tgagcgttac aacattgata ccttcggcct caacgccgtg
    10321 gtgattggcg catcgaatat cgttggccgc ccgatgagca tggaactgct actggcaggt
    10381 tgcaccacca ccgtgactca ccgtttcact aaaaatctgc gtcaccacgt agaaaatgcc
    10441 gatctactga tcgttgccgt cggcaagccg ggctttattc ctggtgactg gatcaaagaa
    10501 ggcgcaattg tgattgatgt cggcatcaac cgtctggaaa atggcaaagt tgtgggcgac
    10561 gtcgtgtttg aagacgcggc taaacgcgcc tcatacatta cgcctgttcc gggcggcgtt
    10621 ggcccgatga cggttgccac gctgattgaa aacacgctac aggcgtgcgt tgaatatcat
    10681 gatccacagg gtgagtaaca tggcgacatt ttctttaggt aaacatccgc acgttgagct
    10741 gtgcgacttg ctgaaactgg aaggctggag cgaaagcggc gcgcaggcga aaatcgcgat
    10801 tgccgaaggc caggtgaaag tcgacggtgc ggttgaaacg cgcaaacgct gcaaaatcgt
    10861 cgccgggcag acagtgagtt ttgcaggtca cagcgtacag gttgttgcct gatccctttc
    10921 ccggcctgta acaacaggcc ggttctcttt tcagactttt gccctctacg ccccgttcat
    10981 ccatgctgat agcatcgaca ttttgccgaa gcgaccatca tgccaaccgt tattacgcac
    11041 gccgccgttc ccctttgcat tggtttaggg ttgggttcga aagtcatccc tccgcgtctg
    11101 ttatttgccg gaatcgtcct ggcgatgctg cccgatgccg acgtgttgtc gtttaaattt
    11161 ggcgttgctt acggcaatgt ttttggtcat cgcgggttta cccattcgct ggtgtttgcg
    11221 tttgttgtcc cgctgttatg tgtgctgatt gggcgtcgat ggttcagggc tgggctgatt
    11281 cgctgctggc tgtttttaac cgtctcgttg ctatcgcaca gcttgctgga ttcggtaacc
    11341 actggcggta aaggggttgg ctggctgtgg ccgtggtcag atgaacgctt tttcgctccc
    11401 tggcaggtga ttaaagtcgc gccgtttgca ttgtctcgtt acaccacgcc gtacgggcat
    11461 aaggtgatta tttctgaatt gatgtgggtg tggttgccgg ggatgctact gatgggaatg
    11521 ttgtggtggc gcagacgata accggatgcg aaaactcgca tccggcaata gcgcaattac
    11581 ttacgacgcc aggtggtccc ttgcgggcca tcttccagca cgatccccat ctcgttaaga
    11641 cggtcacgcg ccgcatctgc cgccgcccag tctttcgctt tacgggcatc cagacgctgt
    11701 tgaattaacg cttcaatctc agccacttca ctgtcgtctg cctgcgcgcc gctttgcagg
    11761 aacgcttccg gttcttgctc cagcaggccc aatacagcgg aaagtttacg caggtgagac
    11821 gccattgcat tcgccgctgc catatcttct gctttcagac ggttcacttc acgcgccata
    11881 tcaaacagta cggaataggc ttccggggtg ttgaaatcgt cgttcatcgc ctcaataaag
    11941 cgcgcttcaa acgcttcgcc accggcagga gcaacggttt tgtctgtgcc gcgcaaggca
    12001 gtatagagac gttccagcgc cgcacgcgcc tgcttcaggt tctcttcgct gtagttcaac
    12061 tgactgcgat agtgacccga catcaggaag taacgcacag tttccgcatc gtagtatttc
    12121 agcacatcac gcacggtgaa gaagttaccc agcgatttag acatcttctc gcggtcaacc
    12181 atcaccatcc ccgagtgcat ccagtagttc acatactgac catcatgggc acaggtggac
    12241 tgggcaattt cgttctcgtg gtgcgggaac atcaggtcag aaccgccgcc gtggatatca
    12301 aagtggttac ccaactgttt gcagttcatt gccgaacact caatgtgcca gccaggacgg
    12361 cccgcgcccc acggagacgg ccagctcggt tcgccctctt tcgacatctt ccacagaacg
    12421 aaatccatcg ggttgcgttt gtcgtcgacc acgtcaacgc gcgcgcctgc ctgcagctga
    12481 tcgagatcct gacgcgacag cacgccataa gttggatcgg tcgggacgtc gaacatcacg
    12541 tcgccgttgt ccgccacata agcgtgacct ttggcgatca gttgttcagt gagttcaata
    12601 atttcggcga tatggtgcgt cgcgcgcggc tccatatccg ggcgcagaat gttcaaagca
    12661 tcaaaatctt tgtgcatttc ggcgatcatg cgatccacca gcgccacaaa gctttcgcca
    12721 ttttcattgg cgcgtttgat gattttgtcg tcgatatcag taatgttgcg cacatacttc
    12781 agcttatagc cgaggaaacg cagatagcgc gcaaccacgt caaaggcaac aaaggtacgc
    12841 ccgtgaccga tatgacagag atcgtaaacg gtgattccac acacgtacat gccgacttcc
    12901 ccggcgtgaa taggcttaaa ttcctctttt tggcgtgtca gagtattgaa gatttttagc
    12961 atcgaagatt ccgtttagac atgtgtgggt aattgagttg cgtataatac ccatatttcc
    13021 cgcgcgaatc agcatacatt gcgtgatgat ccgatcgtgc ggttatgcta taacaccacc
    13081 ctatatatga cccgaaccgg gttgaagcac caatcaaacg gaacaggatg caaaaatggt
    13141 tactttccac accaatcacg gcgatattgt catcaaaact tttgacgata aagcacctga
    13201 aacagttaaa aacttcctgg actactgccg cgaaggtttt tacaacaaca ccattttcca
    13261 ccgtgtaatc aacggcttta tgattcaggg cggcggtttt gaaccgggca tgaagcaaaa
    13321 aaccaccaaa gacccgatca aaaacgaagc taacaacggt ctgaaaaata cccgtggtac
    13381 actggcaatg gcccgtactc aggcaccgca ctctgcaact gcacagttct tcatcaacgt
    13441 ggttgataac gacttcctga acttctctgg cgaaagcctg caaggttggg gttactgcgt
    13501 gtttgctgaa gtggttgaag gcatggacgt ggtagacaaa atcaaaggtg ttgcaaccgg
    13561 tcgtagcggt atgcaccagg acgtgccaaa agaagacgtt atcattgaaa gcgtgaccgt
    13621 tagcgagtaa tcgtggcgac actctttatt gcagatcttc atctctgcgc ggaagaaccg
    13681 gcgatcaccg ccggttttct gcgtttttta gcgggggaag cccgcaaggc cgacgcgctg
    13741 tatattcttg gcgatctgtt tgaagcatgg attggcgacg acgatcccaa cccactccat
    13801 cgccagatgg cggcagcgat caaagcggtg tccgattccg gcgttccctg ttatttcatt
    13861 catggcaacc gtgattttct gctcggcaaa cgctttgccc gtgaaagcgg catgacgtta
    13921 ttgccggaag aaaaggtgct cgaactttat ggtcgccggg tgttgattat gcatggcgac
    13981 acgctgtgca ccgatgacgc cggttatcag gcttttcgcg ccaaagtcca caaaccctgg
    14041 ctgcaaacgc tattcctcgc cctgccgttg tttgtgcgca aacgcattgc cgcgcgaatg
    14101 cgcgcgaaca gcaaagaagc caacagcagt aaatcgctgg cgatcatgga cgttaaccaa
    14161 aacgcggtgg tcagtgcgat ggaaaaacat caggtgcaat ggctgattca cgggcatacc
    14221 catcgcccgg cagtacatga acttatcgcc aatcagcaac ctgctttccg cgtcgtgctg
    14281 ggtgcctggc atactgaagg ttcgatggtg aaagtcacgg cggatgacgt tgagttgatc
    14341 cattttccat tttaaaaaac gcgcaacttt gctgatttca taaccgcgca accgttttcc
    14401 ttgctctctt tccgtgctat tctctgtgcc ctctaaagcc gagagttgtg caccacagga
    14461 gttttaagac gcatgtcttc ccgcaataat ccggcgcgtg tcgccatcgt gatggggtcc
    14521 aaaagcgact gggctaccat gcagttcgcc gccgaaatct tcgaaatcct gaatgtccca
    14581 caccacgttg aagtggtttc tgctcaccgc acccccgata aactgttcag cttcgccgaa
    14641 agtgccgaag agaacggtta tcaggtgatt attgcgggcg caggcggcgc agcgcacctg
    14701 ccaggcatga ttgccgccaa aacgctggtg ccagtgctgg gcgtgccagt acagagcgcc
    14761 gcactgagcg gtgtcgatag cctctactcc atcgtacaaa tgccgcgcgg cattccggtg
    14821 ggtacgctgg cgattggtaa agctggcgcg gcaaacgcgg cgttactggc agcacaaatt
    14881 cttgcgactc atgataaaga actgcaccag cgtctgaatg actggcgcaa agcccagacc
    14941 gacgaagtgc tggaaaaccc ggacccgcga ggtgcggcat gaaacaggtt tgcgtcctcg
    15001 gtaacgggca gttaggccgt atgctgcgtc aggcaggcga accgttaggc attgctgtct
    15061 ggccggtcgg gctggacgct gaaccggcgg cggtgcctgt tcaacaaagt gtgattaccg
    15121 ctgagatcga acgctggccg gaaaccgcat taacccgcga gctggcgcgc catccggcct
    15181 ttgtgaaccg cgatgtgttc ccgattattg ccgaccgtct gactcagaag cagcttttcg
    15241 ataagctcca cctgccgacc gcgccgtggc agttacttgc cgatcgcagc gagtggcctg
    15301 cggtgtttga tcgtttaggt gaactggcga ttgttaagcg tcgcactggt ggctatgacg
    15361 gtcgcggtca atggcgttta cgcgcaaatg aaaccgaaca gttaccggca gagtgttacg
    15421 gcgaatgtat tgtcgagcag ggcattaact tctctggtga agtgtcgctg gttggcgcgc
    15481 gcggttttga tggcagcacc gtgttttatc cgctgacgca taacctgcat caggacggta
    15541 ttttgcgcac cagcgtcgct ttcccgcagg ccaatgcgca gcagcaagcg caagccgaag
    15601 agatgctgtc ggcgattatg caggagctgg gctatgtagg cgtgatggca atggagtgtt
    15661 ttgtcacccc gcaaggtctg ctgatcaacg aactggctcc gcgtgtgcat aacagcggtc
    15721 actggacaca aaacggtgcc agcatcagcc agtttgagct gcatctgcgg gcgattaccg
    15781 atctgccgtt accgcaaccg gtagtgaata gtccgtcggt gatgatcaac ctgattggta
    15841 gcgatgtgaa ttatgactgg ctgaaactgc cgctggtgca tctgcactgg tacgacaaag
    15901 aagtccgtcc ggggcgtaaa gtggggcatc tgaatttgac cgacagcgac acatcgcgtc
    15961 tgaccgcgac gctggaagcc ttaatcccgc tgctgccgcc agagtatgcc agcggcgtga
    16021 tgtgggcgca gagcaagttc agttaattga tgctcatttt cttgccggat gcggcgtgaa
    16081 cgccttatcc ggcccaccgc tccaacaccg actgtaggcc tgataagacg cgccagcatc
    16141 gcatcaggca tggtgtgccg attgcaggat gcggcgtgaa cgccttatcc ggcctaccgt
    16201 tccaacaccg actgtaggcc tgataagatg cgccagcatc gcatcaggca tggtgtgccg
    16261 attgccggat gcggcgtgaa cgccttatcc ggcctaccgt tccgacaccg cctgtagacc
    16321 tgataagacg cgccagcatc gcatcaggct agatgtgcca attgcctggt gcccatcact
    16381 acagcgaaat acaagtcccc gcttcgcccg ccagcgtctc ttcaatccgc gataacgccc
    16441 caatccacgc gggtttgcca cggcttctga catagccact caccgcagtc accttcggcc
    16501 ccatcgaacc atcggctttg gcaaatggcg ctaactcatc cggtgtggca tggcgaatgg
    16561 cacgttgctg cggcgttccc cagttttcat ataccgcatc agcatcggtg aggatcacca
    16621 gtccatcggc attaatctgc tcggcgagca acgcagcggc gagatcttta tcaattaccg
    16681 cttcactccc tgccccgtct ttcgtcacag gcacaccgcc gccgccactg caaatcacca
    16741 catgcccctc tttgagcaac aactcgatgg cttcgctgtc gagaatttta cgcggttgcg
    16801 gagacgccac cacccggcgt aaatatttac catcacgttt catctgccag ccataagccg
    16861 cttccagtgc ctcttgttct tctggctgat aaaccggacc gataaacttc tctggctgca
    16921 aaaacgccgg atcatcaggc gaaacttcaa tgcgcgtcag cactgtcgtg acaggcggca
    16981 tctgcggctg tgcgctcaag ctctgcgcca gcatatagcc aatcatcccc tggctttccg
    17041 ccaccagcac atccagcgga tacggttcta cctctttcca cgccagattc tgtaacgcca
    17101 gcagccccac ctgcggcccg ttgccgtgaa caatcgccag ccgataagaa cgggccaggc
    17161 gcgccagtgc gggtacagca ctggcaatgt tgcgatattg attttctgcc gtcagcgcct
    17221 caccgcgctg gagtaaggcg ttgcccccaa gagccacaac cagtgttttc atgattttcc
    17281 ttgtaataat tgttggccaa gccagaaccc cagcagcgta tctgcccctg acgtatgccc
    17341 cagcgccagc agcgaatcaa tcgcaacagc ggtacgtttc gggcaactca gagcatgaat
    17401 aaagtgcagg agtggcgagg cgaagtatcc ttgcgcggca taacgtaaat aactgacgct
    17461 caccgctgtg gtaacgagtt gaagattgtc ggaacaggca aaaaacgggc gaccggagcg
    17521 cgcatctaaa gcgccataat accaggccgc cagcagcatt ccgctcagcg tgtcatcatg
    17581 acttggcgtt aatcctgggc ctttacccag ccagtgccgc cagtcggtct taacgccatt
    17641 gagcgcggcc tgaaaacagt gacgaaactg gcgtaattca gcaggaagcg gatcgcctgc
    17701 cgccaacgcc agtggcccga aaagcccggt ttcctccgcg cgttgcatcc atgccgctgc
    17761 aagcggttga ggatgcgcag gcggcgtaat acgcagcaaa caataacgct gtggctgttt
    17821 aaccgtgaaa cgtccgaggc gaatcccttg agccataacc tgtggtcgtt cattgccgca
    17881 taatccaccg cataacgcat cgaattgcgc gcggcgaagc acccatcctc cggggccgaa
    17941 accactaccc tgacgatgca gcgttaacag ttcgccgctt tccgtcatca ggttaatcgc
    18001 ccgccgccac actcccgcta accgccagga ctggcgataa ttcggtgcgc tactactggc
    18061 aagcagagga tggatgatcg tcattgtgcg ttcctcaacc aatccccatg ctttcagcca
    18121 gtgcttccag cgcctgctca aagcacgcca gcggtgcccg cacggtgcct gcgccaatct
    18181 gcccgatccc cgcctcttta tgggcgattc cggtattgat gagcggcgta atgccggttt
    18241 ctaccacgcg acgaatatcc agccccaggc acgcgccctg gaaatcccag ccggggatct
    18301 gcaactgcat attgcgttca aggaagattt ccgccatctc ttcagatacc gctctggccg
    18361 cttccatgcc acccgcaccg acaaagcgcg ttacgccagg cgctgcgatc atcgccgcac
    18421 cgccaatacc aaaggtttcg gtaattgcgc tatcgcccat atccgggttc gcctgctcct
    18481 gcgagaagcc ggtgaaaaac agcccttggg gagtgtttac aggcgcagta aaccagcgtt
    18541 cgcccagccc gcttacccga atcccgaaca tattgccgtt gcgggtcata gccgtgacga
    18601 tgctgcctgc gcggatcatc gcaccagcat ccatcgccgc cttgcagtaa gccatcgcga
    18661 ggttaaggaa gaactgatcg gtcacgctga ggaaatccat cacttccgcg atgtgctgtt
    18721 tatcatgatc gaggcgagca atgtgtgggg ccagcgcacg cattaacagt gctgaggaag
    18781 caatattgcg ttgatggaac tcatcgccca tggtaatgcc ctgcgccatc atcgcggtga
    18841 gatcgatacc gcgctccatg cgccccagcg ccgcgcttaa tactggcatt aacacatcgc
    18901 gcatccagcg atggcgagtc agaacatctt cgccgtaagc gccaaaacgc atcactttgc
    18961 cgataccttc gttgaggttg cagtacgccc ggttgccgtc ggtcacgttc tcgaccacca
    19021 gcatcggcat actggcagaa gtaatgccgc ccattggccc gacggcattc acatggtgac
    19081 aaggaatgaa gttcacttcc ccctgatcca gcattgccag cgcctgcgct tcatctttcg
    19141 cccagccttc aaacagacat gcgcccacac atgccccttt catgggtccg gtcatctcct
    19201 gccagcgcat tggcggcccg gcgtgaagca gtgttttgcc ctcgtttagt tcgctgataa
    19261 gtgaagaagc cggttgcaca tccagccagt gtggacgagc gcgacgaatt tgttcgataa
    19321 ccgcagcatt ggcttgcgcc actgatgtaa acatgggaac cccttattgc aaacgttcta
    19381 ataaacgaac cagtttttta ttgccaccgg cgactggcga ccattggtaa tgcacaaccg
    19441 gtttgctggc gctttgtagc tccagcgcaa agctgcgtaa tccgatgtta atcacggcga
    19501 cgttttccag taatgacggt gtatgtggct gtgtggcagg cgagagcgga tgaattaacg
    19561 ccgccgccag caaggtggct tccggcagcg aactcacgac cgcaatcccc gcatcttcca
    19621 gcgtggcgat ttgctgcgag cggcattgcg ggtcacgttc agtgcctgtc accgtggcaa
    19681 tggcgtacag cggttggctg tcagaacgcg cggcacaggc tttttgccag gcgctcacca
    19741 gcgaggcggc aggatcggcg gtcgcaccga agccaatcac gacatcaagc agcaacacgc
    19801 gcacttgcgg tttagcgccg agatcggcaa ttaacaggtt gcgtaaggtc ggatcgatca
    19861 tcggatgggg acgcccgacg gtgtaaaaat catcgcccag gtcgaggatc tgatgtccgt
    19921 cggcgtccag catcatgcca tgttgatggg tatcgtcggc ttccacgcca aggtgtccgg
    19981 caagtaatcc cgccgcttca gcagccagcg taccgccggt atacaagccg caaataaatc
    20041 cgctgctgac aggcgctatt gcgttacgtc gcgcagtgac gcgtgaaagc agacaagcca
    20101 ggcgtgcggc ctcatccagc gaggaggcaa accagacatt ctcgtcgcgg gccaccgccg
    20161 gggtataacc taaaaacagc gccaccgtcg gtttgccagt tgctttcatg gcattaacaa
    20221 ttttcagacg caccgcttcg gcaggtggtt ttgaaacaaa tgccagcact tcgcttttct
    20281 cgtctgcact gagcatttcc agcgccgtca gcgcactgat gccgcccact tcacggctga
    20341 ggtcgcgccc gccaaggcca atcgcgtgag taattccctc ccctgccagc gcaatctgtg
    20401 agcacagctc ctgaatcccg gtaccggaag cgccaatgac gccaatgttg ccttccggta
    20461 tcacgttagc aaaagccagc ggcgtgccgg caatcatcga cgtaccgcag tccggcccca
    20521 tcaccagcaa gcctttttcc cgcgcgcggg ttttaagttg gatttcatct tccagcgtga
    20581 cgttatcgga gaacatcatc acgttgaggt tgcgatccag cgcctggttc gccagctccg
    20641 ccgcatactc accagccact gaaatcagcg ccagattggc atcgggtaat ttctgacagg
    20701 cactgtccca gcgacgcacc tgcgtcaacg cctggctgct ggctgacccc tgcgccagtt
    20761 gttttagcgc ctcttcaagc tgctgcataa tcgcctgcgc gatccccgca tccgccgctt
    20821 cgctacgaat tgccacgcaa atatcgttcg gcgtggcgtt attaaaatcg tcatgccaga
    20881 aacctgtggt atctaataac gctttattgg cgggcgtacc catcattacg gaaacatcat
    20941 caacattttc tgattcgctg agttttcgtg aaataatcat taaactgacc gaatcctgaa
    21001 aacacccttt tttaataaag gcgtggatca taacaactcc ttaaaaattg cacccatggc
    21061 aattgcacga gcagtgttaa tgtggcgatt acggtatcga tgacaacgag taaataaggt
    21121 gat
//
LOCUS       DAZUCC010000072        20948 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZUCC010000072 DAZUCC010000000
VERSION     DAZUCC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.072"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..427)
                     /gene="yiiR"
                     /locus_tag="ACHY09_002312"
     CDS             complement(<1..427)
                     /gene="yiiR"
                     /locus_tag="ACHY09_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS3885279.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADN"
     gene            528..1127
                     /gene="yiiQ"
                     /locus_tag="ACHY09_002313"
     CDS             528..1127
                     /gene="yiiQ"
                     /locus_tag="ACHY09_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFS3885280.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQALLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            1235..2002
                     /gene="tpiA"
                     /locus_tag="ACHY09_002314"
     CDS             1235..2002
                     /gene="tpiA"
                     /locus_tag="ACHY09_002314"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS3885281.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(2057..2812)
                     /gene="cdh"
                     /locus_tag="ACHY09_002315"
     CDS             complement(2057..2812)
                     /gene="cdh"
                     /locus_tag="ACHY09_002315"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFS3885282.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(2918..3907)
                     /gene="sbp"
                     /locus_tag="ACHY09_002316"
     CDS             complement(2918..3907)
                     /gene="sbp"
                     /locus_tag="ACHY09_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFS3885283.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKVFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(4227..5189)
                     /gene="pfkA"
                     /locus_tag="ACHY09_002317"
     CDS             complement(4227..5189)
                     /gene="pfkA"
                     /locus_tag="ACHY09_002317"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS3885284.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5370..6272)
                     /gene="fieF"
                     /locus_tag="ACHY09_002318"
     CDS             complement(5370..6272)
                     /gene="fieF"
                     /locus_tag="ACHY09_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFS3885285.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6421..6921)
                     /gene="cpxP"
                     /locus_tag="ACHY09_002319"
     CDS             complement(6421..6921)
                     /gene="cpxP"
                     /locus_tag="ACHY09_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFS3885286.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7071..7769
                     /gene="cpxR"
                     /locus_tag="ACHY09_002320"
     CDS             7071..7769
                     /gene="cpxR"
                     /locus_tag="ACHY09_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFS3885287.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7766..9139
                     /gene="cpxA"
                     /locus_tag="ACHY09_002321"
     CDS             7766..9139
                     /gene="cpxA"
                     /locus_tag="ACHY09_002321"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFS3885288.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9245..9919)
                     /gene="yiiM"
                     /locus_tag="ACHY09_002322"
     CDS             complement(9245..9919)
                     /gene="yiiM"
                     /locus_tag="ACHY09_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFS3885289.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGVSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10068..11051)
                     /gene="kdgT"
                     /locus_tag="ACHY09_002323"
     CDS             complement(10068..11051)
                     /gene="kdgT"
                     /locus_tag="ACHY09_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFS3885290.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11311..11931)
                     /gene="sodA"
                     /locus_tag="ACHY09_002324"
     CDS             complement(11311..11931)
                     /gene="sodA"
                     /locus_tag="ACHY09_002324"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFS3885291.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12216..13250
                     /gene="rhaT"
                     /locus_tag="ACHY09_002325"
     CDS             12216..13250
                     /gene="rhaT"
                     /locus_tag="ACHY09_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFS3885292.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(13247..14095)
                     /gene="rhaR"
                     /locus_tag="ACHY09_002326"
     CDS             complement(13247..14095)
                     /gene="rhaR"
                     /locus_tag="ACHY09_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFS3885293.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQIIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(14169..15005)
                     /gene="rhaS"
                     /locus_tag="ACHY09_002327"
     CDS             complement(14169..15005)
                     /gene="rhaS"
                     /locus_tag="ACHY09_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFS3885294.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARYLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15293..16762
                     /gene="rhaB"
                     /locus_tag="ACHY09_002328"
     CDS             15293..16762
                     /gene="rhaB"
                     /locus_tag="ACHY09_002328"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS3885295.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTHGLMAQAQRQLGKCDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQSNEIPVVAVASHDTASAVIASPLNSSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            16759..18018
                     /gene="rhaA"
                     /locus_tag="ACHY09_002329"
     CDS             16759..18018
                     /gene="rhaA"
                     /locus_tag="ACHY09_002329"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS3885296.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVARDQIKPEHFKNWVEWAKTNQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
     gene            18203..19027
                     /gene="rhaD"
                     /locus_tag="ACHY09_002330"
     CDS             18203..19027
                     /gene="rhaD"
                     /locus_tag="ACHY09_002330"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS3885297.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNKAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            19037..19351
                     /gene="rhaM"
                     /locus_tag="ACHY09_002331"
     CDS             19037..19351
                     /gene="rhaM"
                     /locus_tag="ACHY09_002331"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS3885298.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPTNPDNSPVSSELQEVFY
                     LP"
     gene            complement(19392..20786)
                     /locus_tag="ACHY09_002332"
     CDS             complement(19392..20786)
                     /locus_tag="ACHY09_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFS3885299.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATSESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         5215 a         5484 c         5204 g         5045 t
ORIGIN      
        1 tattgtcggc tttatacttc acatcctggg tatctttacc atatttattt tcgccttgtg
       61 tgccaacaaa cgcaccaaga tcgataagca tcatcaccag aatcaatgtc gggacaaaac
      121 gccccaccgc ccattgccag acacccggta aaattgccca gttacccgcc agcagcatcc
      181 acgccacaat catcagaaat gcccatgcgc cggaacgccc gcgatcatgc aagcgcttaa
      241 cggttactgc cgctgttggc caaagcaagc acacaaggca aaacgccgcg gtctgaatat
      301 cgagtaaatt tttacccgcc agtgagaaaa gcaccagcat gcctgcgaac cacaggccta
      361 tccaaatcca gaaatcacgg cgtccaatac gccctttaaa tgagaataac cattgctgta
      421 tggtcatgta agttccttga tggttgtctt ttccaggatt ctaccctttt gacaagggcg
      481 acagatatcg ttttaatcgg agccagtcat aacaaaaggt actgtcaatg aagccagggt
      541 gtacgctgtt ttttctctta tgttctgcat taaccgttac aacaacggcg catgcacaaa
      601 caccagatac ggcaacgacc gcgccttatc tgctggctgg agcccctact ttcgatctct
      661 ccatcagcca gtttcgagaa gactttaaca gccagaatcc cagcctgcca ctgaacgaat
      721 ttcgtgccat cgacagcagt cccgacaaag ccaatctcac tcgtgcagca agtaaaatta
      781 acgagaactt gtatgcttct actgcgctgg agcgcggtac cttaaaaatc aaaagcattc
      841 aaatgacctg gctacccatc caggggccag agcaaaaagc cgcgaaagcg aaagctcagg
      901 catacatggc agcggtgatc cgcacactca ccccattaat gaccaaaact caaagccaga
      961 aaaaactgca ggcgctacta acggcgggga aaaacaaacg ttattacacc gagacagaag
     1021 gtgcactgcg ttatgttgtc gcggacaacg gcgaaaaggg gctgaccttc gctgttgaac
     1081 cgattaagct ggcgctatct gaatcgcttg aaggtttgaa taaatgacaa aaagcaaagc
     1141 ctttgtgccg atgaatctct atactgtttc acagacctgc tgccctgcgg ggcggccatc
     1201 ttcctttatt cgcttataag cgtggagaat taaaatgcga catcctttag tgatgggtaa
     1261 ctggaaactg aacggcagcc gccacatggt tcacgagctg gtatctaacc tgcgtaaaga
     1321 gctggcaggt gttgctggct gtgcggttgc aatcgcacca ccggaaatgt acatcgatat
     1381 ggcgaagcgt gaagctgaag gcagccacat catgctgggt gcgcaaaacg tggacctgaa
     1441 cctgtccggc gcattcaccg gtgaaacctc tgctgctatg ctgaaagaca tcggcgcaca
     1501 gtacatcatc atcggccact ctgaacgtcg tacttaccac aaagagtctg acgaactgat
     1561 cgcgaaaaaa ttcgcggtgc tgaaagagca gggtctgact ccggttctgt gcatcggtga
     1621 aaccgaagct gaaaacgaag cgggcaaaac tgaagaagtt tgtgcacgtc agatcgacgc
     1681 ggtactgaaa actcagggtg ctgcggcatt cgaaggtgcg gttatcgctt acgaacccgt
     1741 atgggcaatc ggtactggca aatctgcaac tccggctcag gcacaggctg ttcacaaatt
     1801 catccgtgac cacatcgcta aagttgacgc taacatcgct gaacaagtga tcattcagta
     1861 cggcggctct gtaaacgcgt ctaacgctgc agaactgttt gctcaaccag acatcgacgg
     1921 cgcgctggtt ggcggtgctt ctctgaaagc tgacgctttc gcagtgatcg ttaaagctgc
     1981 agaagcggct aaacaggctt aagtctgaca ggtgccggat ttcatatccg gcacttactt
     2041 tccttaactc ctcgccttaa cgcaaaatct cacactgatg atcctggatt tcctcggctg
     2101 aagcacggtt aagcgtcagt agatttcgtt gtgtcgccag caatacaaat gagttatcac
     2161 tctgccgcac catcgccagc ccgtagcttc ccatatgttc ccgcgcctca ggcacttctt
     2221 ctgcaagcat cataaatggg ctgcgttgta ccagttcgct ttccgttacc cgacgcgcca
     2281 ggtattcatg cccgcgcaaa ccacctggca gtggcaacca gcggctgctg atgttcgcca
     2341 gattgttatc cagctgttcg cgcacatcag gacgaataca agagatatga atatgaaaat
     2401 ggttttgcgt acgcccggtg cgagagttga tcgccaaaga aaccgcgcga tcgggaaccg
     2461 gctggccgta ttttttgctc ataaaatcac gcgcctgcca ggccaaccaa aagaagttcg
     2521 gcgttgaagg atcggtcaac aaaggacttt cagtaccgtt aatacgatac gttggcatca
     2581 acagatattg cagtgggcca ttaagatctt ttaaaaccac gtatccggca ttgggtttga
     2641 cttccgcaca tggcgaagga ttttgatttt gctgctgatt gggcaaacat tcctcaagga
     2701 caatcttacg taatgtatcc gactcttcac cggttaattt ccagtaacca ataccggcag
     2761 ccacaacggc gataactatc atcaccaaaa aaagaagacc cgcttttttc atcttttttc
     2821 cctgtacctc aaagagatgc aagggtaacg caaaatcgtg acaaataaaa aacccagtcg
     2881 cgagaaacca ccgggtttga taattatcct ggagaaatca gcgtttgcta atctgatcga
     2941 acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg aactcttcat
     3001 caatggtgaa taacttcagc tttggaaacg cattttcgta ctttttcgcc acctcagcat
     3061 cgcgcggacg gtagtagttt ttcgcggcaa tttcctgacc ttctggcgag tagagatatt
     3121 tcaggtaggc ttccgccacc tctttggtgc cttttttctc gaccacttta tcgaccaccg
     3181 acacggttgg ctctgcgagg atagactcac tcggcgtgac aatttcgaat ttatctttcc
     3241 ccagttcatt cgctgccagc agagcttcgt tttcccaggc aatcagtaca tcgccaatcc
     3301 cgcgctcgac aaaggtatta gtggagccgc gcgcgccaga atccagaact tcgacgtttt
     3361 tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg tgatgcagcg
     3421 cgtagcccca ggctgccagg tagttccagc gcgcgccacc agagcttttc ggattaggcg
     3481 tgatcaccga aacacccggt ttaatcagat cgttccagtc atggatctgc ttcggattgc
     3541 ccttacgcac caggaaaaca atggtggaag tgtacggtgc ggagttatcc ggcagacgtt
     3601 tgatccactc tttatcaatc cgcccgcgtt ccgcaatcgc gtccacgtca taggccagag
     3661 ccagcgtgac aacatcagct tcaataccgt tgattaccga cgtcgcttgt ttgcctgaac
     3721 caccgtggga ctgacggatc accacgttat cgccagtttg ctgtttccag tgggcgctga
     3781 ataccttgtt gtactgttcg tacaattcgc gcgttggatc atatgaaacg ttaagaagct
     3841 gaatatcctt tgccataacg ctggttgccg ccagcaaaaa tgttaaccct acgccccact
     3901 tgttcatcgc ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc agaaggttaa
     3961 ccaaacatta aagaataaaa aaagattggc tataacttgc gggtatatgt agggggatta
     4021 aaaaggcgga agaaatccgc ctcatattgc tgacaaagtg cgctttgtcc atgccggatg
     4081 cggcgcgaac gccttatccg gcctacaaaa gttcgcaaat tcaataaatt gcagaattca
     4141 tgtaggcctg ataagcgaag cgcatcaggc atttttgctt ctgtcatcgg tttcaggcta
     4201 aaggaatctg cctttttccg aaatcattaa tacagttttt tcgcgcagtc cagccagtcg
     4261 cctttgaacg gacgcttcat gttttcgata gcgtcgatga tgtcgtggtg aaccagctgt
     4321 tcgttctgga tacctacgca acgaccaccg taacctgcca gcagcagctc gatagcgtaa
     4381 gcgcccatac gggaagccag aatacggtcg taaggcaccg gagaaccacc gcgctggatg
     4441 tggcccagca cagttgcgcg ggtttcacga ccggtttctt tctcgatgaa atgcgccagt
     4501 tcgtcaacat cacacatatg ttcggtaatc gccactatcg cgtgtttttt acctttcgcg
     4561 atacccgctt tgatttcgtt taccaggtct tcacggctga attcaacttc cggaaccaca
     4621 acgaattcac agccaccggc aatagccgca gccaaagtca gatcgccaca ataacggccc
     4681 atcacttcca ccacggaaat acgctggtga gaagaagagg tgtcacgcag acggtcgatc
     4741 gcttctacaa cggtgctcag cgcagtgaag aaaccgatag tgtagtcagt gcctttgatg
     4801 tcgttgtcga tagtgcccgg cagaccgatg cacgggaagc ccatttcggt cagacgcatt
     4861 gcacccatgt aggaaccgtc accgccgata accaccagcg cgtcgatacc acgttttttc
     4921 aggttttcga tagccacggc gcggatgttc tcgtcgcgga attccgggaa acgcgcagaa
     4981 ccgaggaacg taccaccacg gttgatcatg tcagaaacgc tgtaacggtc tagctgtacc
     5041 atacggtctt catacagacc cagatagccg tcataaatgc ccattacttc cagaccttct
     5101 gtcagcgcag aacgaacaac cccgcgaatt gcggcgttca tgcctggcgc atcaccgccg
     5161 cttgtcaaca caccgatttt cttaatcatg actacctctg aactttggaa tgcaaaatga
     5221 aatctgttgc cggaagtctt cttgcacatc gaagtgatcc aacgaatgtg caaatagtat
     5281 aacaatcact tcctgctgaa ttgattcagg tcaggccaaa tggcggtatt ttatacacaa
     5341 aatgcgggtc tggctctctt ttatactgat tatgaaagca tagaccgttt accctccctg
     5401 ggtacgacgg aacaggggtc ctgatggata atcacatccg atcccggaaa acgccgtaaa
     5461 atagcctgct ctacctgatc cgccaccata tgtgcctgaa ccaaaggcag agagtcttcc
     5521 atttccaaat gaatctgaat aaagcgggtc ggccctgact gccgcgtgcg aagatcgtga
     5581 gcgccgctaa cacccggcca ggaagtcacg atatcaataa tttcttgccg ttcttcatca
     5641 ggcaatgcgc gatccagtaa tgactgtacc gcctcatatc ccatgcgtaa cgcgctatat
     5701 aaaatataga tgccgattcc caatgcaaac agagcatcgg cgcgatgcca gccgtaccag
     5761 gacaacccca gcgccagcag aattgcaccg ttcatcataa catcagactg gtaatgtagc
     5821 atatcagccc gcaccgcctg gctttgcgtc cggcgcacca cccaacgctg aaacgagaca
     5881 aggataatcg tacaaattag cgccacaatt gtcacgataa ccccgacgcc tggatctgtc
     5941 atcggtgttg gagatatcag atgttgaata cccgtcaaaa acaggaatag tgctgaaccg
     6001 gagataaaca tactttgcgc cagcgccgcg agggactcag ctttaccgtg accaaacgag
     6061 tgattatcgt cggcaggttg cagggaatat cgcaccacca gtaaattcgt caacgacgcg
     6121 ccgatatcca ccagcgaatc caccagcgcg gcgagaatac tcaccgaccc ggtataccac
     6181 catgcaaaaa ttttaatcag caatagcagt gaagccatcg ccgttgcagc aatcgccgcc
     6241 cgactgacca gccgtccata agattgattc ataaatactc ccgctatcaa ctgacgctag
     6301 tataacggaa gcaaatcatc tgcaatgcat taagcagcag gcaaattgag gataaaaaaa
     6361 acccccacat catgtggggg aagacaggga tggtgtctat ggcaaggaaa acagggttta
     6421 ctactgggaa cgtgagttgc tactactcaa tagcttcaac gatgaacttt tttgccattg
     6481 cgtcacgtca cgcaactgct ccattcgttg ttgatgtttc tcatttaaaa ccgcttgctg
     6541 ctccggcgtt aacaggcgat acatttggtt gcggactttt gccatctcaa cctgacgagc
     6601 aatttgctca ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat caaaattttc
     6661 tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca ttaacaggag gctgttcgtg
     6721 ccgggcctgt tgcataagat ctcgcatctg ctgacgctga tgttcggtta aacttatgcc
     6781 gtcgaacata tggctctgcg tactgcgctg cgtaagttct tcacccggat gccagttatc
     6841 gcctgaaccg acttcagcag cgtggcttaa tgaactgact gccagcgttg aggccatgac
     6901 ggcagcggta actatgcgca tcatttgctc ccaaaatctt tctgtcgcga ttcaacgata
     6961 gagagtttac gattcaggct gcaaacatgc gtcagggggt gtaaaacaac gtaaagtcat
     7021 ggattagcga cgtctgatga cgtaatttct gcctcggagg tatttaaaca atgaataaaa
     7081 tcctgttagt tgatgatgac cgagagctga cttccctatt aaaggagctg ctcgagatgg
     7141 aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc gcttgatctt ctggacgaca
     7201 gcattgattt acttttgctt gatgtaatga tgccgaagaa aaatggtatc gacacattaa
     7261 aagcacttcg ccagacacac cagacgcctg tcattatgtt gacggcgcgc ggcagtgaac
     7321 ttgatcgcgt tctcggcctt gagctgggcg cagatgacta tctcccgaaa ccgtttaatg
     7381 atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg agcgaacaac
     7441 agcaaaacaa cgacaacggt tcaccgacac tggaagttga tgccttaagt ctcaatcctg
     7501 gtcgccagga agccaccttc gacgggcaaa cactggagtt aaccggtact gagtttaccc
     7561 tgctctattt gctggcacag catctgggtc aggtggtttc ccgtgagcat ttaagccagg
     7621 aagtgctggg caagcgcctg acgcctttcg accgcgctat cgatatgcat atttccaacc
     7681 tgcgtcgtaa actgccggat cgtaaggatg gtcacccgtg gtttaaaacc ctgcgtggtc
     7741 gtggctatct gatggtttct gcttcatgat aggcagctta accgcgcgca tcttcgccat
     7801 cttctggctg acgctggcgc tggtgttgat gttggttttg atgttaccca agctcgattc
     7861 acgccagatg accgagcttc tggatagcga acagcgtcag ggtctgatga ttgagcagca
     7921 tgttgaagcg gagctggcga acgatccgcc caacgattta atgtggtggc ggcgtctgtt
     7981 ccgggcgatt gataagtggg caccgccagg acagcgtttg ttattggtga ccaccgaagg
     8041 ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta ttggtcaggc
     8101 cgataacgcc gatcatccgc agaagaaaaa gtatggccgc gtggaactgg tcggtccgtt
     8161 ctccgtgcgt gatggcgaag ataattacca actttatctg attcgtccgg ccagcagttc
     8221 tcaatccgat ttcataaact tactgtttga ccgcccgcta ttactgctga ttgtcaccat
     8281 gctggtcagt acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac cggcgcgtaa
     8341 gctgaaaaac gctgccgatg aagttgccca aggaaactta cgccagcatc cggaactgga
     8401 agcggggcca caggaattcc ttgccgccgg tgccagtttt aaccagatgg tcaccgcgct
     8461 ggagcgcatg atgacctctc agcaacgtct actttctgat atctctcacg aactgcgcac
     8521 cccgctgacg cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg gtgaaagcaa
     8581 ggaactggag cgtattgaaa ccgaagcgca acgtctggac agcatgatca acgatctgtt
     8641 ggtgatgtca cgtaatcagc agaaaaatgc gctggttagc gagaccatca aagccaacca
     8701 gttgtggagt gaagtgctgg ataacgcggc gttcgaagcc gagcagatgg gcaagtcgtt
     8761 gacggttaac ttcccgcctg ggccgtggcc gctgtacggc aacccgaacg ccctggagag
     8821 tgcgctggaa aacattgttc gtaatgccct gcgttattcc catacgaaga ttgaagtggg
     8881 ctttgcggta gataaagacg gtatcaccat tacggtggac gacgatggtc ctggcgttag
     8941 cccggaagat cgcgaacaga ttttccgtcc gttctatcgt accgatgaag cacgcgatcg
     9001 tgaatctggc ggtacaggtt tggggctggc gattgtagaa accgccattc agcagcatcg
     9061 tggctgggtg aaggcagaag acagcccgct gggcggttta cggctggtga tttggttgcc
     9121 gctgtataag cggagttaaa ctccgcattt gtaggcagga taaggcgttt acgccgcatc
     9181 cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc ctacactccg
     9241 gggattattt tccccacaat cggcgggaaa aatcttcgat cttgccgctc agtcggcgct
     9301 tttgcatcgt tcgcgtccag cttttcgata cccctgcggc ggagagtaaa cggtgatact
     9361 gatcgtcatc aaacggcata tgccatgcga tggcggcagc ctcctgcacg gtcacatcgc
     9421 tgacacggga aaccaactcc agtggcgcat cagcagatac caaccctggt gcaattacgc
     9481 tatacaacca ccccacttta cccgtgtttt gcatcaactg cgctatatcg ctgatatcaa
     9541 aatggtaatt gagtttatag caaggtgagc gtggctggct gacctgaatt aatgcctctc
     9601 cccagcggaa aatatcgcca atataaacat tactttccgt cagaccgtcg gttgagagat
     9661 tttcgccaaa cgcaggcgca acaaacaact ccgcctgttc cggaaattcc cgcgcccagt
     9721 aaagataatg ctcacgagga taatgacaca gcgctctgtc tggcccgccg tgaacctttt
     9781 tctccgcctg ctcgtcacct tccagcccca ggtccgtcag catcaactca ccatcaacct
     9841 ggattttggc aattgcgctg ggtttgcctt cgggataagc ctgaatcttg cctgtgtata
     9901 catcaaccgg atatcgcatc tgttccctct ccacgagaaa tttccccatt agaccatatt
     9961 tattttatgt caggcgtgct gttttcgcgg taagtatcca ggcaggaact caatagcaaa
    10021 ccggacgaac gttagttcat ccggctgtgg ggattaagga agcaggttta tttcaccgta
    10081 cctaaaattt cgattttcgc tgctctggct ttgactttac gtgaccagat agaggtaaga
    10141 attggcacca gaatcgaagt cacaatgacc gccgtcgcga ccagcgaagt tgctgccgga
    10201 gccatcggtt taaacgcagg caccatttct gcaatcagca caggtgtcgc taccgcggcc
    10261 cctgcggaac tggaagcggc aattccggca gtgccatcgc caccgccaat caatttatca
    10321 gcgataatca acggaatacc ggtcacgata attactgcca cacccaacag gatccccagc
    10381 aaacctgtct gggcaattac cgtcaaatca atggtattac ccagtgcgaa ggcaaagaat
    10441 ggaatcagcg tctgcaccgc tttgctgaaa aattctcgta attcagggtc aaggttccca
    10501 agggcaaagc ccaccaggaa cggtaatact gcgccgacga aaacatgcgg ttcaaacgag
    10561 gcaatcccgg cagtgcccag aataatcatc gtcatgagcg gcccggactc caatgacatc
    10621 aacacaaatg ccccagcttc ttcttttgtg ccgtactgct gcatgatgga agcgtaaagt
    10681 ccgccgttgg tcatatccat cgccgccacc agcgccagcg ttgaaagtcc ggcaaagaat
    10741 ccaacttcaa caccatgttc cggaataatg cgcgaggcaa ttgccgcaac cacccacgcg
    10801 acggcaattt ttgttaccac cagcgtaccg gatttacgca ataccgttcc cgtcgcgctt
    10861 aattttattg acgcccccat gcaaaaaaac cacaccgcca gaatgggcac cgtaccggta
    10921 atcatcccgt tggtgaatga tccaaaatat ttccccgcct ccggcgagaa ggtgtggcac
    10981 agtgcgccaa ggaataacgg gacgagcatc atcccccccg ggattttctc aatcgagcgt
    11041 tttatctgca tttccatcac ctgtcggcat atttttaagg gctgatgcgt cattcctcaa
    11101 tggagcatca gtgttgatgg aaacgaagat agacattcat ttaatcaata aaagtgatca
    11161 aaaccacatc aattgaaaca ctgttttatt tttatcggat cattgttttt attttgaaat
    11221 tgatcacaaa aaaaccaccg ccgttggcga tggttcattt tttaagctga tatgcggcgt
    11281 aaacgcctca ttgcagcagg cggcaaatga ttattttttc gccgcgaaac gtgccgctgc
    11341 ttcgtcccag ttcaccacgt tccagaactc tttgatgtag tcaggacggc ggttctggaa
    11401 tttcaggtag taagcatgtt cccacacatc caggcccaga atcgggaagc cggaagcgcc
    11461 agaaatagct tcacccatca gcggggaatc ctggttagca gtagaaacca ccgccagttt
    11521 atcgcctttc agcaccagcc atgcccagcc ggaaccaaag cgggaagctg ccgctttctc
    11581 aaattctgct ttgaagttat caacggagcc gaagtcacgt tcgatagccg ctttcaggtc
    11641 accctgcagg gtggtgcctt ttttcagacc tttccagaac aggctgtggt tagcgtgacc
    11701 gccagcgttg ttgcgcagta cggttttctt gtctgctggc agctggtcca gtttggtgat
    11761 cagctcttca accggcaggt tggcaaattc tggcaggctt tccagcgccg cgttggcgtt
    11821 gttgacgtag gtctgatggt gtttggtgtg gtggatttcc atggtctgct tatcgaagtg
    11881 cggttccagg gcatcgtaag catacggcag ggatggcagg gtataactca tattcatctc
    11941 cagtgttgtc gggcggccga ttgttaatgc cgcgtaagca gttggttcat tatagttaat
    12001 taaatgatat tgaaaatgat tatcaatgcc gtacttttcg taagggtatg gttttgcagg
    12061 aaaatgcccg agatgtgaag caaatcaccc acttaatgcc gtgattgcca gtaaatcgac
    12121 aacggcggca acaggcgaaa ggttaatcga cagcacgatt tttacactca tctcgtcgga
    12181 gatgtgacgc gacgaaaaat gatgaggata agaagatgag taacgcgatt acgatgggga
    12241 tattttggca tttgatcggc gcggccagtg cagcctgttt ttacgctccg ttcaaaaaag
    12301 taaaaaaatg gtcatgggaa accatgtggt cagttggtgg gattgtttcg tggattattc
    12361 tgccgtgggc catcagcgcc ctgttactac cgaatttctg ggcgtattac agctcgttta
    12421 gtctctctac gctgctgcct gtttttctgt tcggcgcaat gtgggggatt ggtaatatca
    12481 actacggcct gaccatgcgt tatctcggca tgtcgatggg gattggtatc gccattggta
    12541 tcacgttgat tgttggtacg ctgatgacgc caattatcaa cggcaatttc gatgtgttga
    12601 tcaataccga aggtggacgc atgacattgc tcggcgttct ggtggcgctg attggtgtag
    12661 ggattgtgac tcgcgccggg cagttgaaag agcgcaagat gggcattaaa gccgaagagt
    12721 tcaacctgaa aaaagggctg gtgctggcgg tgatgtgcgg cattttctct gccggaatgt
    12781 cctttgcgat gaacgccgca aaaccgatgc atgaagccgc tgccgcactg ggcgtcgatc
    12841 cactgtatgt cgctctgcca agctatgttg tcatcatggg cggcggcgcg atcattaacc
    12901 tcggtttctg cttcattcgt ctggcaaaag tgaaggattt gtcgctaaaa gccgacttct
    12961 cgctggcaaa accgctgatc attcacaatg tgttactctc agcactgggc gggttgatgt
    13021 ggtatctgca attctttttc tatgcctggg gccacgcccg cattccggcg cagtatgact
    13081 acatcagttg gatgctgcat atgagcttct atgtattgtg cggcggtatc gtcgggttgg
    13141 tgctgaaaga gtggaacaat gcaggtcgcc gtccggtaac ggtgttgagc ctcggttgtg
    13201 tggtgattat tgtcgccgcc aacatcgtcg gcatgggcat ggcgaattaa tctttctgcg
    13261 aattgagatg acgccactgg ctgggcgtca tcccggtttc ccgggtaaac accaccgaaa
    13321 aatagttact atcttcaaag ccacattcgg ttgaaatatc actgattaac aggcggctat
    13381 gctggagaag atattgcgca tgacacactc tgacctgccg cagatattga ttgatggtca
    13441 ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc actgcacgat gcctcatcac
    13501 aaaatttatc cagcgcaaag ggacttttca ggctggccgc cagccgggta atcagcttat
    13561 ccagcaacgt ttcgctggat gttggcggca acgaatcact ggtgtaacga tggcgattca
    13621 gcaacatcac caactgcccg aacagcaact ctgccatttc gttagcaaac ggcacatgct
    13681 gactactttc atgctcaagc tgaccgataa tctgccgcgc ctgcgccatc cccacgctac
    13741 ctaagcgcca gtgtggttgc cctgcgctgg cactaaatcc cggaatcgcc ccctgccagt
    13801 caagattcag cttcagccgc tccgggcaat aaataatatt ctgcaaaacc agatcgttaa
    13861 cggaagcgta ggagtgttta tcgtcagcat gaatgtaaaa gagatcgcca cgggtaatgc
    13921 gataagggcg atcgttgagt acatgcagac cattaccgcg ccagacaatt accagctcac
    13981 aaaaatcatg tgtatgttca gcaaagacat cttgcggata acggtcagcc acagcgactg
    14041 cctgctggtc gctggcaaaa aaatcatctt tgagaagttt taactgatgc gccaccgtgg
    14101 ctacctcggc cagagaacga agttgattat tcgcaatatg gcgtacaaat acgttgagaa
    14161 gattcgcgtt attgcagaaa accatcccgt ccctgacgaa tatcacgcgg tgaccagtta
    14221 aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccgca gcgataggcg
    14281 atgtcagtaa cgctggcctc gctgtggcgt agcagatatc gggctttcat cagtcgcagg
    14341 cggttcaggt aacgctgagg cgtcagcccc gtttgctgct taagctggcg atgcagcgta
    14401 cgcaatgaaa gagaaaattg atccgccacg gcatcccaat tcacctcatc ggcaaaatgg
    14461 tcctccagcc aggccagaag caagttgaga cgtgacgcgc tgttttccag gttctcctgc
    14521 aaactgcttt tacgcagcaa gagcagtaat tgcataaata agatctcgcg actggcggtc
    14581 gagggtaaat cattttcctc ttcctgctgt tccatctgtg caaccagctg tcgcacctgc
    14641 tgcaacacgc tgtggttaac gcgccagtga gacggatact gcccatcctg ctcttgtggc
    14701 agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata cagcacattg
    14761 gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg tacgaaacag
    14821 accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc cgtgccatgt
    14881 tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc ctgcgggagc
    14941 cggggttcta tcgccacgga cgcgttacca gacggaaaaa aatccacact atgtaatacg
    15001 gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag gtcaggttct
    15061 taccttaaat tttcgacggg aaaccacgta aaaaacgtcg atttttcaag atacagcgtg
    15121 aattttcagg aaatgcggtg agcatcacac caccacaatt cagcgaattg tgaacatcat
    15181 cacgttcatc tttccctggt tgccaatgac ccattttcct gtcagtaacg agaaggtcgc
    15241 gaattcaggc gctttttaga ctggtcgtaa tgaaattcag caggatcaca ttatgacctt
    15301 tcgcaattgt gtcgccgtcg atctcggcgc atccagtgga cgcgtgatgc tggcgcgtta
    15361 cgagcgtgaa tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca atgggctgca
    15421 tagccagaac ggttatgtca cctgggatgt ggatagcctg gaaagtgcca ttcgccttgg
    15481 attaaacaag gtgtgcgagg aagggattcg tatcgatagc attgggattg atacctgggg
    15541 cgtggacttt gtgctgcttg accaacaggg tcagcgcgtg ggcctgcctg ttgcttatcg
    15601 cgatagccgc acccatggtc tgatggcgca ggcacaacgg caactcggca aatgcgatat
    15661 ttatcaacgt agcggcatcc agtttttgcc cttcaatacg ctttatcagt tgcgtgcgct
    15721 gacggagcaa caacctgaac ttattccaca cattgctcac gctctgctga tgccggatta
    15781 cttcagttat cgtctgaccg gcaagatgaa ctgggaatat accaacgcca cgaccacgca
    15841 actggtcaat atcaatagcg acgactggga cgagtcgcta ctggcgtgga gcggggccaa
    15901 caaagcctgg tttggtcgcc cgacgcatcc gggtaatgtc attgggcact ggatttgccc
    15961 gcagagtaat gagattccgg tggtcgccgt tgccagccat gataccgcca gcgcggttat
    16021 cgcctcgccg ttaaacagtt cacgcgccgc ttatctctct tctggcacct ggtcattgat
    16081 gggcttcgaa agccagacgc catttaccaa tgacacggcg ctggcagcca acatcaccaa
    16141 tgaaggcggg gcggaaggtc gctatcgggt gctgaaaaat attatgggct tatggctgct
    16201 tcagcgggtg cttcaggagc ggcaaatcaa cgatcttccg gcgcttatcg ccgcgacaca
    16261 ggcacttccg gcttgccgct tcaccatcaa tcccaatgac gatcgcttta ttaatcctga
    16321 cgagatgtgc agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccaa tcccggaaag
    16381 tgatgctgaa ctggcgcgct gtattttcga cagtctggcg ctgctgtatg ccgatgtgtt
    16441 gcatgagctg gcgcaactgc gcggtgaaga tttctcacaa ctgcatattg tcggcggtgg
    16501 ctgccagaac gcgctgctca accagttatg tgctgatgcc tgcggtattc gggtgatcgc
    16561 cgggcctgtt gaagcctcga cgctcggcaa tatcggcatc cagttaatga cgctggatga
    16621 actcaacaat gtggatgatt tccgtcaggt cgtcagcacc accgcgaatc tgaccacctt
    16681 tacccctaat cctgacagtg aaattgccca ctatgtggcg cagattcact ctacacgaca
    16741 gacaaaggag ctttgcgcat gaccactcaa ctggaacagg cctgggagct ggcgaaacag
    16801 cgtttcgcgg cagtagggat tgatgtcgag gaggcgctgc gccaacttga tcgtttaccc
    16861 gtttcaatgc actgctggca gggcgatgat gtttccggtt ttgaaaaccc ggaaggttcg
    16921 ctgaccggtg ggattcaggc tactggtaat tatccgggca aagcgcgtaa tgccagcgag
    16981 ctacgtgccg atctggaaca agctatgcgg ctgattccgg ggccgaaacg gcttaattta
    17041 catgccatct atctggaatc agatacgcca gtcgcgcgcg accaaatcaa accagagcac
    17101 ttcaaaaact gggttgaatg ggcgaaaacc aatcagctcg gtctggattt taacccctcc
    17161 tgtttttcgc atccgctaag cgccgatggc tttacgcttt cccatgccga cgacagtatt
    17221 cgccagttct ggattgatca ctgcaaggcc agccgccgtg tttcagccta ttttggcgag
    17281 caactcggca caccgtcggt gatgaacatc tggatcccgg atggcatgaa agatatcacc
    17341 gttgaccgtc tcgccccgcg ccagcgtctg ctggcagcac tggatgaggt gatcagcgag
    17401 aagctggatc cggcgcatca tatcgatgcc gttgagagca aattgtttgg cattggcgcg
    17461 gagagctaca cggttggctc caatgagttt tacctggggt atgccaccag ccgccagacg
    17521 gcgctgtgcc tggacgccgg gcatttccac ccgactgaag tgatttccga caagatttcc
    17581 gccgccatgc tgtatgtgcc gcagttgctg ctgcacgtca gccgtccggt tcgctgggac
    17641 agcgatcacg tagtgctgct ggatgatgaa acccaggcga ttgccagtga gattgttcgt
    17701 cacgatctgt tcgaccgggt gcatatcggc ctcgacttct tcgatgcctc tatcaaccgc
    17761 attgccgcgt gggtcattgg tacacgcaat atgaaaaaag ccctgctacg tgcgttactg
    17821 gaacctaccg ctgaactacg caagctggaa tcggcgggcg attacactgc gcgtctggcg
    17881 ctactggaag agcagaaatc gttgccgtgg caggcggtat gggaaatgta ttgtcagcgt
    17941 cacgatacgc cagcgggtag cgaatggctg gagaacgtgc gggcttatga gaaagaaatt
    18001 ttgagtcgcc gcgggtaaac actgccggat gcggcacaag tgccttatcc ggcctacggg
    18061 tcggcaatag ttgtaggcct gataagacgc gacagcgtcg catcaggcat tgattgccgg
    18121 atgcggcacg agtgccttat ccggcctaca gatcgtatat ccggcaacag gcacggacgt
    18181 ccaattaaca ggaacaccga acatgcaaaa cattactcag tcctggtttg tccagggaat
    18241 gattaaagcc accaccgacg cctggctgaa aggctgggac gagcgcaacg gcggcaacct
    18301 gacgctgcgc ctggatgacg ccgatatcgc accatatcac gacaattttc accaacaacc
    18361 gcgctatatc ccgctcagcc agcccatgcc tttactggca aatacaccgt ttattgtcac
    18421 cggctcaggc aaattcttcc gtaacgtcca gcttgatcct gcggctaact taggcatcgt
    18481 aaaagtcgac agcgacggcg cgggctacca cattctttgg gggttaacca acaaagccgt
    18541 ccccacttcc gaacttccgg ctcactttct ctcccactgc gagcgcatta aagccaccaa
    18601 cggtaaagat cgggtgatca tgcactgcca tgccaccaac ctgatcgccc ttacctacgt
    18661 actggaaaac gacaccgcgg tcttcactcg ccaactgtgg gaaggcagca ccgaatgtct
    18721 ggtagtgttc ccggatggcg tcggcatttt gccgtggatg gtgcccggca ccgacgaaat
    18781 cggccaggcg accgcacagg aaatgcaaaa acattcgctg gtgttgtggc ccttccacgg
    18841 cgtcttcggc agcggtccga cactggatga aacattcggt ttaatcgaca ccgcagaaaa
    18901 atcagcacaa gtattagtga aggtttattc gatgggcggc atgaaacaga ccatcagtcg
    18961 tgaagagctg atagcgctcg gccagcgttt cggcgttaca ccactcgcca gtgcgctggc
    19021 gctgtaagga gcaaacatga tccgcaaagc ctttgtcatg caggttaacc ccgacgccca
    19081 cgaagagtat cagcgtcgtc ataatccaat ctggccggaa ctggaagcag tgttgaaatc
    19141 tcacggtgcg cataactacg ccatttacct cgacaaagcg cgtaatctgc tgtttgccac
    19201 ggtagagatt gaatctgaag aacggtggaa tgcggtagcc agtactgatg tttgccaacg
    19261 ttggtggaaa tatatgaccg acgttatgcc aactaacccg gataacagcc cagtcagtag
    19321 cgagctgcaa gaagtgtttt acctgcctta atgagttgcg gtgccggaaa atatccagca
    19381 ccgcgacttc atcagaagaa gtatttaaag cgaacgcgtg caccgtaacg gtcatcagta
    19441 tcatcaccgt gttttttatc accgtcgcta tccagaatgg agtaatacgt cccgaggtaa
    19501 atattaaagt tctccatatc catcacatta gcgaactgat aagacgcatg aatggtgtga
    19561 atggtgtacg tcccttcatc atcaatccag cagtcgttat cacaaactac accgctgaat
    19621 tcatcaatct tattgtgcgc gtagatataa cccagctcga aacgtttcca cagcgcgtta
    19681 atcccggcag tgaaatcttt ctcattatta gcatctaaat aggcggtatt aagattaacc
    19741 acgacaccat tttccggatc cgttttcagg ccattccagg tcatgctcat accatagccg
    19801 gtacgatcgg actgatcgac aaaattaccc ttgctatcgg tataaccata agcattattt
    19861 accacgttcg cttccatcgc tgcggaaacg gtgaattctt ccgtcggcga ccaggcaatt
    19921 accggacgca gataagcaac atttttctgc tgttccatat cgcgtccatg ataattaccg
    19981 tcgttatata aagatgttcc gtcttccagt aacgtgttta attcaaaata ccagttatcg
    20041 agttgtttgc tgacgaggaa attaccgcca gcgttagaac gcccgcggcc ctctttcatc
    20101 atatagatat aaccgctgcc atcgtcataa agatcgttcg cagtattacc ggaatgttca
    20161 acaaaggtat cctgattcag cgggaacata tcgtaggctt caaaacggcc tactttgacc
    20221 ttccagtcgt tttctttgcc aaagaagaat accgcatcat cgatatttac tgaaccgtgc
    20281 atgtccccca gcggttgtgc ggagaacccg gcgaaataac cgttgtccat ctttcgcata
    20341 ccgtcaaaac ctaacagaat acgaccattg agattccact gttcattagt gggatcgcta
    20401 ttcacatcag cgttggtcat tgccagaagg ccatgattac tttccgcatc catattaaat
    20461 tctacgtcgc cgtagatttt taaatcgcca taacctgaaa gcaaaagatt aggtgaggtg
    20521 gaatttttct ttgcagattc cgacgttgcc tgtccattta ccggcgtatt gccctgggca
    20581 gccttaattt cgcggatctc agcggcttgt tgtgtctgca gctgtttaac ctggctctct
    20641 gcttttgctg cccgcatttc tgccgcttcc aggcgttctt ccagcaatgc catgcgttgt
    20701 tccagcgtta aatgctgtgt ttttgccatt ccggtactac atgccaataa taaaccgatg
    20761 gctaaagata atgtcgattt tttcattatt tacgttccct gaaataaaag agcccctacg
    20821 ctttccaaaa ggaatacgca tatagagaaa tataaggtgt gcactttcga atttttaatc
    20881 caacttcagc aggccgatgg atagtatccc gccatataat atgagtaaat ccgctgattt
    20941 cctggaac
//
LOCUS       DAZUCC010000073        20943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZUCC010000073 DAZUCC010000000
VERSION     DAZUCC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.073"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(85..615)
                     /gene="cybB"
                     /locus_tag="ACHY09_002333"
     CDS             complement(85..615)
                     /gene="cybB"
                     /locus_tag="ACHY09_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFS3885300.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            804..1805
                     /gene="gap"
                     /locus_tag="ACHY09_002334"
     CDS             804..1805
                     /gene="gap"
                     /locus_tag="ACHY09_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS3885301.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILAKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(1846..3285)
                     /gene="aldA"
                     /locus_tag="ACHY09_002335"
     CDS             complement(1846..3285)
                     /gene="aldA"
                     /locus_tag="ACHY09_002335"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFS3885302.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISTLIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3482..4282)
                     /gene="ydcF"
                     /locus_tag="ACHY09_002336"
     CDS             complement(3482..4282)
                     /gene="ydcF"
                     /locus_tag="ACHY09_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFS3885303.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEMPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(4554..8456)
                     /gene="hrpA"
                     /locus_tag="ACHY09_002337"
     CDS             complement(4554..8456)
                     /gene="hrpA"
                     /locus_tag="ACHY09_002337"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFS3885304.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELSLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAIEQISG"
     gene            8657..9262
                     /gene="azoR"
                     /locus_tag="ACHY09_002338"
     CDS             8657..9262
                     /gene="azoR"
                     /locus_tag="ACHY09_002338"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFS3885305.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(9316..10608)
                     /gene="ynbD"
                     /locus_tag="ACHY09_002339"
                     /pseudo
     CDS             complement(9316..10608)
                     /gene="ynbD"
                     /locus_tag="ACHY09_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
     gene            complement(10622..12379)
                     /gene="ynbC"
                     /locus_tag="ACHY09_002340"
     CDS             complement(10622..12379)
                     /gene="ynbC"
                     /locus_tag="ACHY09_002340"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFS3885306.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLASSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIISDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALVFEKMQ
                     SFISHLYANKSQKLDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDIFLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRGN"
     gene            complement(12395..13291)
                     /gene="ynbB"
                     /locus_tag="ACHY09_002341"
     CDS             complement(12395..13291)
                     /gene="ynbB"
                     /locus_tag="ACHY09_002341"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS3885307.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWVTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGLNC
                     FELFLLFIPLFGFLILATWRVFVGEPSGFLHTVSAIFCGWIMTVFALSHAAWLLMLPT
                     TNIQGEALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     VSLIIGPLLTPLDTLQALLAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(13291..13896)
                     /gene="ynbA"
                     /locus_tag="ACHY09_002342"
     CDS             complement(13291..13896)
                     /gene="ynbA"
                     /locus_tag="ACHY09_002342"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS3885308.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLHKRQVSANQITLAALALSLLTGL
                     LLALVPYHGMFLLLPIVLFVRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWVL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMNAEK"
     gene            complement(14065..15405)
                     /locus_tag="ACHY09_002343"
                     /pseudo
     CDS             complement(14065..15405)
                     /locus_tag="ACHY09_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
     gene            complement(15469..16329)
                     /gene="pdxI"
                     /locus_tag="ACHY09_002344"
     CDS             complement(15469..16329)
                     /gene="pdxI"
                     /locus_tag="ACHY09_002344"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFS3885309.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLGALDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(16560..17150)
                     /gene="paaY"
                     /locus_tag="ACHY09_002345"
     CDS             complement(16560..17150)
                     /gene="paaY"
                     /locus_tag="ACHY09_002345"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFS3885310.1"
                     /translation="MPIYQIDGLTQFVPEESFVHPTAVLIGDVILGKGVYVGPNTSLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIHRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRSKQTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            complement(17132..17284)
                     /locus_tag="ACHY09_002346"
                     /pseudo
     CDS             complement(17132..17284)
                     /locus_tag="ACHY09_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
     gene            17285..17491
                     /locus_tag="ACHY09_002347"
                     /pseudo
     CDS             17285..17491
                     /locus_tag="ACHY09_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(17663..17989)
                     /gene="ydbL"
                     /locus_tag="ACHY09_002348"
     CDS             complement(17663..17989)
                     /gene="ydbL"
                     /locus_tag="ACHY09_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFS3885311.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(17997..18182)
                     /gene="ynbE"
                     /locus_tag="ACHY09_002349"
     CDS             complement(17997..18182)
                     /gene="ynbE"
                     /locus_tag="ACHY09_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFS3885312.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(18179..20818)
                     /gene="ydbH"
                     /locus_tag="ACHY09_002350"
     CDS             complement(18179..20818)
                     /gene="ydbH"
                     /locus_tag="ACHY09_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFS3885313.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVVARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGHLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWSE
                     DEPLLLTDVSVDVLGGNVLMKQLRMPQHDSALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNAHLPGNDCPQGKECEDKQ"
BASE COUNT         5478 a         5548 c         4749 g         5168 t
ORIGIN      
        1 ataataggcg cttccgatgt agacccgtat tcttcgcctg taccacgggt cggttttagt
       61 acaggcgttt tctttaaata tccttcagga acgtttacgc ggcatcatgc gtagaagtgt
      121 gttgtccttc cagaaatagt ggtgtgccag tgcagccgca gcgtgcaacc cgatgacaaa
      181 atatcccaga ttcgccagca tttcatgcca cgactttaag ctatctaccc gttcgaaatt
      241 ggcctctgaa gcgtaaggca tcgtcaaacc aaacgcaaac cacgggttgc cccggttata
      301 catcatcacc aaaccaatca ctggcagcgc aataaacagg agataaatca ccaaatggcc
      361 caaatgcgcc agtcccgtca tcatcggttt tggcttaggt ataatcggcg gggttgggta
      421 tttcagtctt aacaaaagac gaaccaccat cagtacgaga attgagatgc cacaggaaac
      481 atgaatcatg ttgatgaggg gccgatcgct acgtgggaag aaaccacgaa actccattgc
      541 gcaatacgct acgataacca gcaaaaagac cagccagtga atgctgattt gtaaccttga
      601 gtatttattt cccataacat ttcctgcttt aacataattt gccgttaaca taacgggctt
      661 ttcttaaaat ttcattaaat attgttcacc cgttttcagt acgttacgtt tcactgcaag
      721 aatgtagatt gcccatcccg ccatcctggt ctaagcctgg aaaggatcaa ttttcatccg
      781 aacgttcctg acaggagaag accatgagta aagttggtat taatggtttt ggtcgtatcg
      841 gtcgactggt gttgcgtcga ttacttgaag tcaaaagcaa catagacgtt gtcgctatta
      901 atgatctcac ttccccaaaa attctcgcct acctgctgaa acatgattca aactacggac
      961 cgttcccctg gagcgttgat tttacggaag attcacttat cgttgatgga aaaagcatcg
     1021 cggtttacgc cgaaaaagag gctaaaaata ttccgtggaa agcgaaaggg gcagaaatta
     1081 ttgtcgaatg tactggcttt tatacctccg ccgagaaatc gcaggcgcat cttgatgctg
     1141 gtgcgaagaa ggtgttgatt tccgcccctg ccggtgaaat gaaaactatc gtttataacg
     1201 tcaatgacga cactctggat ggcaacgaca ccattgtttc cgtggcgtca tgcaccacta
     1261 actgccttgc gccgatggcc aaagccttgc atgacagttt cgggatagaa gtcggcacga
     1321 tgacgaccat tcatgcctat accggcaccc agtcactggt ggatggtccg cgtgggaagg
     1381 atttacgagc ttcacgcgca gcggcagaaa atatcattcc ccacactaca ggtgcggcaa
     1441 aagccattgg tctggtgatc ccggaactga gcggcaaact gaaaggtcat gcgcaacgcg
     1501 tgccggtgaa aacaggttcg gtcactgagc tggtgtccat tctcgcaaaa aaagtgactg
     1561 ccgaagaggt gaataacgca cttaaacaag caaccaccaa taacgagtca tttggttata
     1621 ccgatgaaga aatagtctct tccgatatca ttggcagcca tttcgggtcg gtgtttgatg
     1681 ccacgcaaac ggaaattacc gccgtgggcg atttacaact ggtgaaaacg gtcgcctggt
     1741 acgataacga atatggcttc gtcacacaac tcattcgcac cctcgaaaaa ttcgctaaac
     1801 tctgacgcac acaggcggag gaaaaacctc cgcctcttcc actcattaag actgtaaata
     1861 aaccacctgg gtctgcagat attcatgcaa gccatgtttg ccatctgcgc cgccaatacc
     1921 agatttacgc catccggcgt ggaatccttg catagcttcg aagttttcac ggttgatgta
     1981 agtttcacca aacttcagcc ctttaatggc tttcatcgcg acgttcagat tttgggtata
     2041 gattgatgag gtcaggccgt aatcactatc attcgccatt gagatagcct cttccagcgt
     2101 gtcaaatgcg accaccggca gaaccgggcc aaaggtttcc tcatgcataa tcgacatttc
     2161 ctggcgaaca tccagcagca atgtcggcgg ataataatat cctttcccct ctaccgcttt
     2221 gccacccaac gccactctcg ccccttcttc tactgcgcgc gccacttttt gctcgaccct
     2281 ttccagcgcc gcggcgttaa tcaacggccc catcgcaatg tcgttgcgtt cagcggggtt
     2341 accaaattga accgcctgca tcgcttcacc cagccgattg acaaactgat cataaatgcc
     2401 tttctgtaca taaacacgtt ctgcacagtt acacacttgc ccactattaa tgacgcgtga
     2461 atcaacgatg gctttgactg ccagttcaag atcggcatcg tccattacga tagccggtgc
     2521 tttacccccc aattccaggc acactttggt gatgtttttc gccgcagtcg ccataatctt
     2581 ttcacctgca gagacgctgc ctgtcatact gaccattgcg acctttgggt tacccgccag
     2641 ttcttgccca acggtttcac cacgccccag aacaaggtta aacacgccgc gcggaaggcc
     2701 tatttcatcg acaattttgg cgaatgcaat cgcattgttt ggcgtaaatt cactgggttt
     2761 aatgacgatg gtattaccgg tcaaaagagc gggagccatt ttgcgggcaa tgaggaagaa
     2821 cgggaagttc cacggcagaa tgccggtagt cacgccaagc gcacgtttaa acagaagaat
     2881 attttctcct ggacgatcgc tttgaataat ctcgccttca taacgccgtg cccactccgc
     2941 catgtaatcg atatagtccg cagtaaaagc cacttcgact tcagccagct gctggatctt
     3001 acccccttct tcaacaatca gcgtactgat ttcactggcg cgttcgcgga tcccggcgga
     3061 gattttgcgc aaccaactgg cgcgttcaat agcaggcaac gcttcccatt ctggttgtgc
     3121 acgttctgct gcatcgattg ccttacgggc atcctcggcc tgaccatcgg gaatgcggga
     3181 aatgacagcc tctgtagcag ggtttaccac atcaatccat gcgtctccac gccaggtaac
     3241 aaactgtcca tcaatataca taggatgttg aacgggtact gacatgagcg actcctgtga
     3301 tttatatgtt tgtttttggt aaatgtattg ttaattaaca tcaatcatca tagagacggc
     3361 atcatgaaag gacaattctg tgaagggctt cataaaaaca agtataaaag gaataagagg
     3421 cagaaataaa aggcaggagt tatgcccgtt tgcaaacaag tggttcacaa acgggcaatt
     3481 tttaacgtaa cgagcgactt tccatcgcct cgattagcac cgcatcgtgt ttcagcgttt
     3541 gccatgcatg gatgacttct gccggaaaat caacgtgaac gataaaatct cgcccgcggg
     3601 gaccgtagcc atcgctatca tcgcgtaaac gcggcagctc gccagtgagt agtgagagat
     3661 aacgctcaac cggccataat ccttgcaacg gattaacaaa gattacactg tctgcgttat
     3721 ttcctaactg aggaacgaat ccgggataac ttaaccagcg tggtgcatcg ggattgtccc
     3781 cagtcatacg gcggaacgtc gccatcgtgc gccgctgcat ggtggggtcc tgaacaacga
     3841 tagccgtatg aactcgttct acggcctgat tcaatagcgc gatgctaaag cgtgcgtttt
     3901 caccgcagtt tgttgactgg tcttcaatcc agattttttc atgcggaatg tgccagaact
     3961 gatgagcgat atccgccagg atggtcgctt ctgctctgcc agtggtgcgg atagtgttgt
     4021 agtgcggatg ctgtgcgatg gcgctataca aaaaagttgt cgagtgaccg ataccaccac
     4081 taatcagtag aggaatttgc tgatcgcgag caatcttaca tgccgcgtcg atagtcggca
     4141 taaccgcatt gcctgcaagg atcacgcaat cggcctgata cggcatctca ccggagaaat
     4201 catcctgcgc cagccactga ccgataacat ttatggcatc tatggttgcc ggcgaaagcg
     4261 tcggaaacgg ggtaatgttc atggttcctc cttatcgttc ccttaagaat actgcgtgag
     4321 ataatagtga gaagcggagc actctgaaag gaggaagtcg tatttcctta accgccactc
     4381 cgcaagcctg acatatcttt taaggaaatg ctgtagataa acattattca tcttgcagct
     4441 gagaattgta tttttctgaa ttcctgaatt tttgccctta ccgttttaga attcataaaa
     4501 aacccggcgc taaatgctta ccgggtttta gatttatcag gcaaatagcc gcgttaaccg
     4561 ctaatctgtt ctatcgcctg caaaatacgc ttatctgaaa tcggataagg cgtaccaagt
     4621 tgttgagcga agtaactaac gcgcaactct tctatcatcc aacggatctc tttcacgtct
     4681 tcatcctcac gacgtgcggg cggcagtttg ttgatccatt gctgccacgc ctgctggaca
     4741 ttttcgactt tcagcatctg agcacggtca cgatgcggat caaccgccag tttttccagt
     4801 cgtttttcaa tcgcctgcaa ataacgcagc gtgtcgccca gccgtttaaa accgttacca
     4861 gtgacaaaac cgcgatacac cagcccgccc atctgcgctt tgatgtcaga aagccccagc
     4921 gccatagtca tatccacccg ccctttcagg cgtttgttga tattgaacac tgccgtaagg
     4981 atttgctcga cctgcttcgc aatatccacc accgtatcgt tcagttcagc gcggactttc
     5041 tcatgcagcg cagcaaagcc ttcttccgtc cagaccgggc ctccattggc gtcgatcagt
     5101 tgatccaccc cgcaggagat acagtcgtcg atcagctcca gcactttgcc atacgggtta
     5161 aagtacagcc ccagcttggc tttgttcggt aacttttcat gcaaatactt gatcggcgat
     5221 ggaatattca gcagcagtaa acgacgaaga ccgttccaca tcgcttgctt ttgttccagc
     5281 gggttatcaa acagtttgat cgccacgctg tcgcgctcat ccaccaacgc tggccacgcc
     5341 ttcactttgt agttgccacg cttctgttcg tagctttccg gcagttgccc aaagctccag
     5401 atatgtaagc cgctctgctc gataccgtca tccgccaccg cagacagcgt ttcctgcact
     5461 ttgcctttca gagcatcttt cagatcctgt aatgagcgcc cttcttttag cttcttgttt
     5521 ttgtcatcca ccacacggaa ggtgattttc aggtgatcgg gcacctgatc ccagtgccag
     5581 tcttcgcggt caacggtgac gccagtcatc cgccgtagct ctcgttcaag actgtcgagc
     5641 aacgacaatt ccaacggtgt gacgcggcct agaaacgctt cggcgtagtt tggcgcgggc
     5701 acaaaattac ggcgtactgg tttcggcaac gatttaatca gagcaatcac cagttcgcgg
     5761 cgcagaccgg ggatttgcca ttcaaacccg ctctcttcta cctgattaag cagcggcagc
     5821 ggaatatgca cggtcacacc gtccgcgtcc gcgcctggct caaactgata gctcaaacgc
     5881 agcttgagat tgccctgatg ccagaagttc ggataatcca gcttgctgat tttttccgcc
     5941 ccttctttga tcaacatgct cttttcaaag ttgagcaaat caggcgtttc gcggctgact
     6001 tttttccacc agctatcgaa gtgacgagcg gagattacat cgtggctgat gcgctggtcg
     6061 tagaactcaa acagcgtttc gtcatcaacc agaatatcgc ggcgacgtga tttgtgttcc
     6121 agctcttcca cttcggcccg cagtttcagg ttttcacgga agaatgcgtg acgcgtctgc
     6181 cagtcacctt ccaccagagc gtggcgaata aagagttcac gacataacgc cggatcgatc
     6241 tggctgtagt tgaccttacg cgcagcaaca atcggcaaac cataaacagt gactttttcc
     6301 gttgccatca ccgcgccctg cgcccgttcc cagtgcggtt cgctgtaggt gcgtttaatc
     6361 aaatgctgag caacgggttc tacccattca gggtcgatac gcgcagcaat gcgcccccac
     6421 aggcggctgg tttctaccag ttccgccacc attacccatt tcggcggctt tttgaataaa
     6481 ccagaaccgg gaaagatgga aaaacgcgcg ttacgtgcgc cggtatattc ttgtttatcg
     6541 gcatctttca tgccgatatg ggaaagcaaa cccgtcagca acgcaatgtg aatttcgcga
     6601 tactccgccg gttcgctgtt aaccggaatg ccaagttctt tcaccacctg gcgcaactgg
     6661 gtgtagatat cctgccattc gcgcacgcgc agatagttga gataatcggt acgacacagg
     6721 cgacggaagg cgttagaaga aagcgccttt tgctgctcgc cgagataatt ccacagattt
     6781 acaaacgcga ggaagtcgga ttctttgtcg tggaagcggc gatgtttttc gtctgatgcc
     6841 tgctgtttat ccatcggacg ctcgcgcgga tcctgaatgg agagcgcgga cgtgataatc
     6901 atcgcctcac gcacgcagcc atgtttttgc gcttccagca ccatacgcgc cagacgagga
     6961 tcgacaggca attgcgaaag ctggcgaccg agcggcgtca gtttataggc gctggcctgt
     7021 tcatcagtgg tgatcgcgcc cagctcttcg agcagacgca cgccatcctg gatattacgt
     7081 ttatccggtg cttcgacaaa tgggaacgcg gcgatatcgc ccaaccccag cgcggtcatc
     7141 tgcaaaataa ccgaggccag gttagtacgc agaatctccg gatcggtaaa ctccgggcgc
     7201 gagaggaaat cgtcttcgga atagagacga atacagatcc cttcggacac acgaccacag
     7261 cggcctttac gctgattggc agacgcctga gaaatcggct caatcggcaa acgctgcact
     7321 ttagtgcgat agctgtagcg gctgatacgc gccgtacctg ggtcgataac gtatttaata
     7381 cccggtacgg tcagcgacgt ttccgcgacg ttggtcgcca gcacaatgcg ccgtccactg
     7441 tgcgactgga acacgcggtt ctgttcgctg ttcgaaagcc gcgcataaag cggcaagatt
     7501 tcggtatggc gtaagttcag cttgttcagc gcatcggcgg tatcgcgaat ttcccgctca
     7561 ccactcataa agatcagaat gtcgccaggg ctttcctggc tcagttcgtc tacggcgtca
     7621 aaaatcgcct gcagctgatc gcgctcggtg tcatcggctt cttcaacaat cggtcgatag
     7681 cgcacttcca ccggataggt ccgaccggag acttcgataa tcggcgcatt attaaagtgg
     7741 cgcgaaaagc gttccgggtc gatagtcgcg gaagtgataa tgatttttag gtcaggacgc
     7801 cgcggtagca actctttcaa atagccgagc agaaaatcga tattcaggct acgttcgtgc
     7861 gcttcgtcaa taatgatagt gtcgtactgc atcagcaggc ggtcttgctg gatctccgcc
     7921 agcaggatac cgtcggtcat cagcttgacc atcgtgttat cactaacgtg atcactgaaa
     7981 cgcactttgt aaccgatgca accgcccggc tccgttttca gctcttccgc aatacggttc
     8041 gccaccgttc gtgccgccag gcgacgcggt tgggtatgac cgatcagccc tttaatcccg
     8101 cgccccagtt ccatacagat tttcggtagc tgagtcgttt taccggaacc ggtttccccg
     8161 gcgacgatca ccacctggtg atcacgaatc gcttcgagta tgtcctgctt tttctgacta
     8221 accggcaaat tgtccggata agtgatttcc ggtcgtgccg cttcacgcag caggactttc
     8281 cctgccgcat ggtcaatctc tttcgccatc tcctggaaaa tggcctgttg tgcatcagga
     8341 tttttaacct tcttcacgcc gtgcagacgg cgagaaaaac gcagtctgtc gcgcagcatc
     8401 agcgaatcca gccgctgctg caaggccgta aaggtcaatt tttgttgttc tgtcataacg
     8461 ttagtgggca gtagtctgcc tgattaattt tcttttaaat gatagatata gagtaccaca
     8521 tcgcggcttt ctatgcgttg ttcaataaat tcgaacatag gcttcgattt attgcgctat
     8581 ctctgtggca ggattgtgaa taaagtgtca acaagcaacg gggcatcgcc cattcaaaca
     8641 tctataagga aacaccatga gcaaggtatt agttcttaaa tccagcatcc tggcagggta
     8701 ctctcagtct aatcagttgt ccgattattt tgttgaacaa tggcgcgaaa agcactccgc
     8761 tgatgaaatc accgttcgcg acctggctgc aaatccgatt ccggtgctgg atggcgaact
     8821 ggttggtgcg ctgcgtccga gcgatgcacc gctgactccg cgtcagcagg aagctctggc
     8881 actttccgat gagctgattg ccgagttgaa agcccacgac gttatcgtta ttgcggcacc
     8941 gatgtataac ttcaacatct cgactcagtt gaaaaactat tttgacctgg ttgcacgcgc
     9001 aggcgttact ttccgctata cggagaacgg tccggaaggc ctggtcaccg gtaaaaaagc
     9061 catcgttatc accagccgcg gtgggatcca taaagatggc ccaacggatc tggtgacgcc
     9121 gtatctgtcc acattcctgg gctttatcgg cattaccgat gtgaaatttg tcttcgccga
     9181 agggatcgca tacggtcctg aaatggcagc gaaagcgcag tctgacgcga aagcagccat
     9241 cgacagtatt gttgctgaat aactggacac gatcttgatg tggactgtag atattcagtc
     9301 cacatctcaa tccgcttacc tgttttccca taatttcagc atcgctttgt gctcttcctt
     9361 aagcacaatc cgcgagcgtc tggctcgaat ataactaatc gcttcatcaa cggttttaca
     9421 atgtccgtaa cataacagcc atgccgccac caccagcgca ctgcgcgata atcccaacgc
     9481 gcaatggacc agaacgctgc cttgctcttc gcgtaatgtt tccagcatcg ccacggcctg
     9541 tcggagctcc ccctcttccg gaaccaccag atccagcatc ggtacacaaa aatagagttg
     9601 atcttttgtg gctcgtccgc gagggaattc aaaggtgacg tccagaacag cattctgtgc
     9661 cggaatatgt tgtggaaacg cccctaaata aacaccagca gtaattttgc tcaccgactc
     9721 caggcgaaga caaaaccaac gcatagacag ccacatcccg atgcgccagg gcaatgtcag
     9781 ccagtaaacg gcgggcggga gtttccccta actatctttc cctgttgtta tcgcgccaag
     9841 cccaccgtac ccacggccaa tgatgaatag cgataatact ggccaacata accagactga
     9901 ccaccataac tgaagcatta tcattagctc catcaacaca atgcacgagc acgcgcctac
     9961 gacatatggc agtgctattt tgattcgacg ttgatcaggt ttctgataat tccaacgacg
    10021 gtcgacgggc accatccagt caattaacat acctaccgcc agccctgtga tgacatcaat
    10081 aaaatgatgc tgccaggtcg tcagcgtcga aatggcgatg agtaaaaacc atccgccgca
    10141 gactttacgc cacctcacag ccagatgctg acgaaagtga cgccaaagta gccagcagag
    10201 aataatatgc agcgaaggag actggttata aggcagatca aacaattcaa gttgcgaaaa
    10261 taaccatcct gtcaccccac tcacttcagg acggataaaa ctaaacttca gcggatagag
    10321 aaaaaaaccg cagcaggcca ttaccgttgc cagaataagc cggtggacca gacggcgctg
    10381 ctcaaatgtc gagctacaaa caaacagcga aaatccatat aaaagatcca gactccagta
    10441 aggaacaata gtccagggaa gaaaagggat cgccgtttcc caaccgaata actgactggg
    10501 gatatcatgg ttgttaagat cctgagtcgc ggtgaactga ttaagaaatc catatgtgaa
    10561 gaagaaaaac ggggccagca acaataacca gccagcgcct tgtagcaata cgttccggcg
    10621 ttcagttacc acgacgcacc gccatcgaaa ccgtaaaaat gccccactca tcaatccgtt
    10681 gtgtgcattt atcgaatccg gcatcatgca cgagcgaatc catctcccct tgagaacgca
    10741 cgcgcattac ccacggttta ccatctttat gactggttaa caccccggca atcagctcca
    10801 gttgtgggtg ccacggctgc ccggtgtaga tgagaatgcc acccggatcg atggcattgg
    10861 caagacctgc gagtgagttt tttacctgct cattttcggg aaaaagctca tacagtccag
    10921 agacaatcgc cagcgtaggc cgcggagtta acgcgctgag ctcctccggg ttaaacgcat
    10981 cgccctgttc aaaacgcacc cgcccagaca ttccccgttg ggcaatcatc tcttgcccct
    11041 gtgcaacatt taactcactg taatcacgta agaaaatatc gcttacggca ggctcgttcg
    11101 ccagcgcatc cagtacatag cgtccatgcc ctgcggcaat gtcaaccacg cggacggcta
    11161 aacctttagc gtggagatcg gcaacggctt gtttaatcag catttgtaaa tgggttttgc
    11221 gctggcgaat accgcgccag ccaacactgt tcaggtagat tttgtcgact aaacgcccga
    11281 atgcgttact accttgcggt tgattttgat agacataatc cagcgaactg cctgaatcaa
    11341 agccggtgct cattccgaga tgcaggcccg cagagtgcgc cccgaacaat ttcatcgctt
    11401 tacgcataaa gcgatacgcc aaatcaaccg gcgataatgg cacgggtcca ccagaaagta
    11461 gccgccatcg atccgctgat ggtcctgtgc agtcttcatg ctgataatct aatttttgcg
    11521 atttgttagc atataagtga ctaataaagc tttgcatttt ttcaaatacc agcgccctgt
    11581 tctcttcacc caacgtgtcg tgataaaagc ctggcagcag atgcagctct ttcagagggc
    11641 tacgtaaacg ctgataaaaa tcaatctgcg gttggcggtg caccacatag tcatcgcctg
    11701 atatcagaag ttgcgtgggg agcgtaatcg ccgccgcatc gctaataata cgttcagacg
    11761 ttttatagag atctagcaag atattgacgg caatcgccct tgtgatcagc ggatcattat
    11821 tgaaactagc cacccgttgc cgatcgtggg tcaaatagcg tcctttcaca taggaattaa
    11881 taaaaaacag accacgcaga cgatgccata acgccagcgc aggacgtgcc agcggcacat
    11941 acaatttaac cttaaaggcc ggagaagcca gcaccagccc gcgaatagca ggcgcataat
    12001 catgaaccca tgtagcaacc atcactgcgc cgacgctttg cgcgatcaca accacctctt
    12061 ccagtccgac ctggctgtcg ctggcagcaa aacggacaaa ttcatcgaca tcttgcactg
    12121 aactcgcaag agacggacta taaccacgcg gccccgaagt ttgtccatgc cctcgggcat
    12181 cccatgcata aaaagcagta tctggcatcg ccagttcatc aacgagatgt tgtagacgac
    12241 cagaatgttc atgaccgcga tgaaataaga cgatgacctt tttcgcccct ggctgtaaag
    12301 tgggccagtg ccgataaaac aacgctgtat tatcactggt ggtaaaaaaa tgttccccag
    12361 ggatgcgtga attttccatt ctctctattt tccttcagta acagcaatag cgaataaaat
    12421 aaaaaaatac cggagcggtg aaaattaatg agtcaatcct gtcaaggagt ccgccatgtc
    12481 ctggtaatag ttttccacta tctttaacac caacatctcg tttgatggct gacatcacta
    12541 catcgccgca aaaaccacta ataccaatta acaaacccgc taataatgcc tgtaatgtat
    12601 ctagcggtgt cagtaacggt ccaataatca gtgacacaat catggtagtg attacgcccc
    12661 ccaccagacc ttccagcgtt tttcccgggc tgactttggg gaccactttt cttctgccgc
    12721 aggatttccc ccataaatac tgtgcaatat cgtttgactc ggttaatgcc aaaagaaata
    12781 acaccagtaa cgcctcgccc tggatatttg tggttggcaa cattaataac caggcggcat
    12841 gactcaaggc gaaaacggtc attatccagc cacaaaaaat ggcgctcacg gtatgcagaa
    12901 aaccggaggg ttcacccaca aaaactcgcc aggtggctaa tatcagaaac ccaaacaaag
    12961 gtataaataa cagaaacagc tcaaagcaat ttaaaccgat aagcaaatag ttaagaggaa
    13021 taacccaata taaccaacgc ggaaaatgta cggatataag cgtacaatat tctttcaatg
    13081 ccagaaaact tatcagcgca aaaaacgtca acgtcaccca gcgtggacct gaaatcacca
    13141 gtgaaaaaca aatgacgatg ccccaccagg tacgaatacg taatgtaact tcatcccctt
    13201 ttctctccgg taaccgccat accagaaaac ggtttatcag cgttgctaaa attaacaacg
    13261 cgaaaagtgt tgccagagat ttttccagca ttatttttca gcgttcatca ggagcacact
    13321 ccgacaacga ttaatcgcag tccagagaag cagaattgaa gcaatgctcc ataaaagatt
    13381 attccattgc atccactgtg gataaatggc aacggccagc acccacaacc caaatattaa
    13441 cgcgcgatcg cttttgccaa atggaccggc ataactgcga ataccgttaa tcgtctgcgc
    13501 gagtaaaccg caaaactcag tcaatatggt gcaaaataac atgagtatta cgagtgatgc
    13561 gttactttcc ggtaaaaata aaaacggtaa ataaagcgca atatcggaaa taacatcgcc
    13621 agtttcattc aaaatcgccc caagacgtgt ttgctgattg cactcacgcg ccatcatgcc
    13681 atccagcgca ttgagtgcca tacggacaaa aagcacgatg ggcaatagca gaaacatgcc
    13741 atgatatggc accaacgcca acaacaaacc ggtgagaaga gaaagtgcca acgcggcaag
    13801 ggtgatctga tttgcagaca cttgccgctt atgaagccaa aacatcgtcg gccttaacag
    13861 tgactgaaag agcggtttta tctggtagag cgtcattatc aatccttgat aattctgttt
    13921 gcgctatggt gcatcaattg ccctggcaga gatatcgcaa cacgccgctt cattaaaaat
    13981 tcagaatata tcaaggtatt ggggctcact gtcaggaatg attatcccca attaatcaaa
    14041 tagttaaaaa tattacgctg ttattcaatg caactcatca agttttctca tcgcttgttc
    14101 ccgtattgcg cgcgtcttac cgttaagcgc cagctggcgg actaaatcca gttcagggtt
    14161 aacaagctct aacgccagca aaggatcacg tttcagcctc gcctttaata catcaactgg
    14221 aaaacgggga ttattcatcg ccacaatcca ccactcggga tcaatttctg ccgccagtac
    14281 tgcaggcggt gtgtggcggt tgttaagtgc gccgaacttg aaaaatgtga gattgctttt
    14341 cgccagtgta ctccaccata tacgatccgg ttgttttgcc agtacatcca gttgtgctaa
    14401 cgcatcgggt tctagtgcat aataaacacg taacgcttcg ttaacctgcg cacgggaact
    14461 gttttcctgc ctcagaaaaa acttcgccca aatatcattt cgtggcgtgt tttgctgtaa
    14521 ttcctttaag ccttgggaga gttcgcatta tggcaagatc aaatgtcgca atgtctcgtc
    14581 cttcaaatgc agcaacgttg ccttcatcca gcatttgcaa ttcctgcaat aattcaggag
    14641 aagacttagg gcgaaacagc gcttcttttt tcatctgatt acagacattc cctgctatgc
    14701 gaatattgct tcgcttaaaa atttctttca gagcatcttc atcatttatt aggccccaat
    14761 aatatgcatc aggatttatt atcatcagcg cttttttgac attttcagtg tcatttttgt
    14821 cactcgcacg gaccatgaat tcactatttg tcgaagatgc aacaacaggt gcaattatgt
    14881 cattgataaa tatcccgcgc gcattaatac ttcccttaat cgtagacgga ttatcgtaat
    14941 atgagtcgat aaataccagt ccacttgcat ttatcgaacc ggtaattaac agtggtgaat
    15001 ccgtaactat attcgttgcc gttacgttac gttacgttac gttacgttac gttacgttac
    15061 gttaccgaga ataacccatg gattagaaag agagaaagtg gaaatattac ctttgactgt
    15121 caaattgcca agaacaatca atttttgatt actctctata ttaccttcta ccattaaatc
    15181 gccacgatgc actttccagt ccgatgcaga taaaccgacg gggacaagat tcacgctact
    15241 gttgatagct tgttcaactt gttgtttatc ttcagcggta gccgcagaaa aaaatggttc
    15301 aatatcaact gcatttacaa cacatcgaag ttccgcctgt actggaagac tcagcataaa
    15361 caggggaagt aaacctatcc agctgcaaat cctgggctta aacatatgca ctccatgcca
    15421 ttaaaaaaga aacaaaaggg aaatgcatct ttcccttttg tatattcgtt attctcgcga
    15481 aataccgtcc aacgtagaca acacttcctc agaaagatgc aatttttcag cagccatatt
    15541 ctcccgtaaa tgcgcaacag aagacgtccc tgggatcagc aaaatattcg gtgaacgctg
    15601 taacagccac gccagcgcca cctgcattgg tgttgcgccc aggctcgctg ctacatccga
    15661 aagtgtggag gattgcagcg gcgtaaagcc cccaagcgga aagaacggca cgtaggcaat
    15721 gccgtcacga gccaaagcat caatcattgc gtcatcagca cgatgagcaa tgttgtattc
    15781 gttctgcaca cagacgattt cggtaatctt acgcgcctct gcaacctgcg tcggtgtaac
    15841 gttgctcaga ccaatatgtt ttaccaggcc ttgttgttgc atctctgcca gcacggtcag
    15901 gctggcttca atcgatcctt ccgctgggcc atgaccatcc cccatcataa cgcgcaggtt
    15961 aaccacatcc agtgcgccca gcccgagatt acgtagatta tcgtgcaccg ctttttgcag
    16021 ctctgccgga gaaaatgcgg gcaaccagga tgcgtcctct ccacgccgcg caccaatttt
    16081 agtgacaatt gtcaggtcgt cagagtaagg ataaagcgct tcgcggataa tctgattggt
    16141 gacgtgcgga ccataaaagt cgctggtatc aatatgattg acgcccaatg ccagcgcctc
    16201 acgcagcacg gttatagcga cgtggcgatc tcttgggggg ccaaaaaccc caggacctgc
    16261 cagttgcatc gcgccataac caagacggtt aacggatttt gtaccgagag taaatgtatt
    16321 gctgctcatc gcaccctgcc ctgtaataaa acggattaag aaaaatagta tagaaacgaa
    16381 aagagataaa taatgcgaag gcgtaaacaa gattactgga tgagttgctc tattgccctg
    16441 aaagacacca aaacaaaacg cgtcaagtac cgcatccggc aataaaaaaa ccgcaacctg
    16501 tatcagcgtt gcggtttagg tatcacatgc taaaaattag aacgtccagc atcaggacgt
    16561 tactgtttcg gtcgcagatt ctcatcaaat accaggcgct gcctgccagg ttcaacttcc
    16621 cgcaatggct caacttgatg caacgtctgc ttactgcgtg tcaccagcac ctgatactca
    16681 tgcgtgccct gctttttcca tgccagttcc tgctcactaa gttcacgaat cgcttttgcc
    16741 gggctgccga caatcaggta attagcaggc atttctgctt tggctttcac aaatgcggaa
    16801 gcgccaacaa tgctgttctc gccaatcacc gcaccgtcca tcactaccgc gttcataccc
    16861 actaatgcat tgcggtggat aatgcagccg tgaaggatag cgctatgacc aatatgccca
    16921 tcttcttcca cgacagtatc ctgatcggga aaaccgtgca taacgcaatt atcctgaatg
    16981 ttcgcgccat ctttcaccac gatacgaccg aaatcgccac gcaggctggt atttggccca
    17041 acgtacacgc ccttgccgag aataacatcg ccgatcaata ccgctgtcgg atggacaaaa
    17101 ctctcttctg gcacaaactg agtcagaccg tctatctgat aaattggcat aacgcctcct
    17161 gttcaatatt caagccgcca aaacgttgaa aatacaggct tcccggcgca ggcaattcac
    17221 cgaccgaggt ttcacctttt tcactaacga acgctaaagc agcaggcgct acgcgctgat
    17281 aaagtttgcc gataaaggtt tggtagtcgc gcaatatatc cgtaaccgtc gtctcgattt
    17341 ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca ttggctttca
    17401 ttggggattt tccgaccaga gtcatttttc aacggtattt aagcaacgct ttgggatgac
    17461 gccaggcgag tatcgacgta aattccgcta actaattcgc gttgcatgta cagcaacgcg
    17521 aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa tttgacagga
    17581 atttttgaat attcacgttg tttccggaaa atagattaat cgtcttctcc tgtagcgtta
    17641 tcccgatatt gcggttttgc tattaaaatt ttcgcaccca tttaccgttt atcccttgta
    17701 catattcccc gggtttggcc cgttctacaa gcttttgccc ggcgagtttc gcgatatcat
    17761 ccaccgacac attattctgc tttgccagtt gttgatagct cgctttacgt tcggcattga
    17821 tgtcggttac taatttctca gtttcagcat ctgttttcag cgcaaccaga taaccataaa
    17881 atgtttcgcc tacccgcccc tgagttctgg cttcatccag agtcaatgcc attacattgc
    17941 tgcttaccaa gccaacaaga aatgcacaaa gaagtaatgt tcttttcatc atcacctcaa
    18001 aaaagatcgc tacgggtctc aagcagctct tcgacatctt tgtctgcctt gatgatgatc
    18061 tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc agcgacttca
    18121 atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag taaaattttc
    18181 attgtttatc ctcacactct tttccttgcg gacagtcatt tcccggtaga tgcgcgttct
    18241 gctccagcca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat
    18301 tctcttcatg atggtaatta agatttaccg taccgctttt accatcaacg cgactggtac
    18361 ctgtaatatt ggcctgcatg gttaataaac cgagattatc taaattaatt tttgtcgatg
    18421 aacggctaat ttccatatag cgcaaccagt taattgctga acccgcggtc atattgtctt
    18481 tcaccaccgc atctgcggtg tctttatcca gccgcaaggt cattggcccg ctatttgcca
    18541 gccagccgtc tttcactatc catttttcgt tgtttagcca taacggcaat gcaccactaa
    18601 atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaga
    18661 gattattcag ccgcaacagc gccgagtcat gttgcggcat gcgtaattgt ttcatcagca
    18721 cgttaccgcc taacacatcg acgctaacat cagtcaacag caatggttcg tcttcactcc
    18781 acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt
    18841 cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac
    18901 ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc
    18961 ctttcaacac accgtgccct cccgcgcgga atccttgttc aggcgcagct gaaaatgcaa
    19021 cctgagcata cagttcgcca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct
    19081 ggaatacagt cagtgattgt ttaggccacc aggcgttacc gcgcagacga ataccgtccc
    19141 agcgaccatt tacccgaacc gggccaatct caccagcatg caaatcgcct ttaaagagga
    19201 aatacgtcgg atcgcgccca tcgacactaa attttaaggt tgatggcggt aacacactgc
    19261 cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctaaag gagggatgct
    19321 gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt tctaccgtca
    19381 tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta atcgtgctgt
    19441 catgccactc accctttcct gcgacatccc aggtggcatt catcggtgta aaactgcctt
    19501 ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc gccagtccat
    19561 ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaat
    19621 gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga
    19681 tgtccagcga atcgatgact cttcccttcg aacgtaataa cgcgcctggc tcaaaggcca
    19741 gcgttgggtc agtcagactt ccgcttagct gcgcaggtaa acgggcatat aaaatgaggt
    19801 ccgcctgttt cgcttcaccg gtcagctgca aaggcagctg gctgttatcc atacttaatt
    19861 ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg gtcagcacac
    19921 tcagtcgtcc gctgaccaga gcgttctcga gccctgcctg ccagttgtcg actttgacac
    19981 ccagtcgtcc actcaaagga aaacctgcat acggccagct ccagcgacca tcgcttacgg
    20041 tcaattgttg gcgagtaatt tgccacggca gatcgagcaa cggatcgccg ttatcccgtg
    20101 ccaccacaat caattgcccg ctattttcct gccagtccag ctcggcatcc accagtgaag
    20161 gctcctgcgg caagtttaac gtcgcagtag catgaccact tacaggaagt ccatccggca
    20221 ctagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca aacgcgacga
    20281 catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt
    20341 cgccctgata acgcagttgc tggatatcgg aggttaatgc gagagagagt tttccctgcc
    20401 attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcaacatgg
    20461 cctgccactg cgcgagggtt tttggagcgg caggcgattg ctccgtctgc ggcaatttcg
    20521 ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg
    20581 aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg
    20641 gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga
    20701 gcggtagcca gatgcccgcc agcgtgggaa cccacagccc gagcgtcatc agcagtgtca
    20761 acggcacaag aataatcagt aataatagcg cgagaacggc tttatattta cccagcatgg
    20821 gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt
    20881 taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata
    20941 tcg
//
LOCUS       DAZUCC010000074        20874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZUCC010000074 DAZUCC010000000
VERSION     DAZUCC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.074"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..101
                     /locus_tag="ACHY09_002351"
     tRNA            25..101
                     /locus_tag="ACHY09_002351"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Arg,seq:acg)
     gene            382..948
                     /gene="yqaB"
                     /locus_tag="ACHY09_002352"
     CDS             382..948
                     /gene="yqaB"
                     /locus_tag="ACHY09_002352"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFS3885314.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            945..1373
                     /gene="yqaA"
                     /locus_tag="ACHY09_002353"
     CDS             945..1373
                     /gene="yqaA"
                     /locus_tag="ACHY09_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFS3885315.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1446..3002
                     /gene="gshA"
                     /locus_tag="ACHY09_002354"
     CDS             1446..3002
                     /gene="gshA"
                     /locus_tag="ACHY09_002354"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFS3885316.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFIAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3152..3667
                     /gene="luxS"
                     /locus_tag="ACHY09_002355"
     CDS             3152..3667
                     /gene="luxS"
                     /locus_tag="ACHY09_002355"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS3885317.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3718..4830)
                     /locus_tag="ACHY09_002356"
     CDS             complement(3718..4830)
                     /locus_tag="ACHY09_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3885318.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALVTQPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(4827..5534)
                     /locus_tag="ACHY09_002357"
     CDS             complement(4827..5534)
                     /locus_tag="ACHY09_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFS3885319.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINADYWQDLQAITQLVHTEK
                     LDGENNCLNRYGVFARSHAAPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGAFDPWDTQTGQPCTLEGIVSRNRDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFDYGEQS"
     gene            complement(5792..7330)
                     /gene="emrB"
                     /locus_tag="ACHY09_002358"
     CDS             complement(5792..7330)
                     /gene="emrB"
                     /locus_tag="ACHY09_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFS3885320.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(7347..8519)
                     /gene="emrA"
                     /locus_tag="ACHY09_002359"
     CDS             complement(7347..8519)
                     /gene="emrA"
                     /locus_tag="ACHY09_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFS3885321.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(8646..9176)
                     /gene="emrR"
                     /locus_tag="ACHY09_002360"
     CDS             complement(8646..9176)
                     /gene="emrR"
                     /locus_tag="ACHY09_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFS3885322.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(9267..9602)
                     /gene="ygaH"
                     /locus_tag="ACHY09_002361"
     CDS             complement(9267..9602)
                     /gene="ygaH"
                     /locus_tag="ACHY09_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFS3885323.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(9592..10329)
                     /gene="ygaZ"
                     /locus_tag="ACHY09_002362"
     CDS             complement(9592..10329)
                     /gene="ygaZ"
                     /locus_tag="ACHY09_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFS3885324.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(10453..11637)
                     /locus_tag="ACHY09_002363"
     CDS             complement(10453..11637)
                     /locus_tag="ACHY09_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3885325.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRTL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLPWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASVWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AI"
     gene            complement(11829..12821)
                     /gene="proX"
                     /locus_tag="ACHY09_002364"
     CDS             complement(11829..12821)
                     /gene="proX"
                     /locus_tag="ACHY09_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFS3885326.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(12879..13943)
                     /gene="proW"
                     /locus_tag="ACHY09_002365"
     CDS             complement(12879..13943)
                     /gene="proW"
                     /locus_tag="ACHY09_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFS3885327.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(13936..15138)
                     /gene="proV"
                     /locus_tag="ACHY09_002366"
     CDS             complement(13936..15138)
                     /gene="proV"
                     /locus_tag="ACHY09_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFS3885328.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(15494..16453)
                     /gene="nrdF"
                     /locus_tag="ACHY09_002367"
     CDS             complement(15494..16453)
                     /gene="nrdF"
                     /locus_tag="ACHY09_002367"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS3885329.1"
                     /translation="MKLSRISAINWNKITDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(16463..18607)
                     /gene="nrdE"
                     /locus_tag="ACHY09_002368"
     CDS             complement(16463..18607)
                     /gene="nrdE"
                     /locus_tag="ACHY09_002368"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS3885330.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSSDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDIERVYGKPFADVAISEHYAEL
                     VANERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSSITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(18580..18990)
                     /gene="nrdI"
                     /locus_tag="ACHY09_002369"
     CDS             complement(18580..18990)
                     /gene="nrdI"
                     /locus_tag="ACHY09_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS3885331.1"
                     /translation="MSQIVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(18987..19232)
                     /gene="nrdH"
                     /locus_tag="ACHY09_002370"
     CDS             complement(18987..19232)
                     /gene="nrdH"
                     /locus_tag="ACHY09_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS3885332.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAANA"
     gene            complement(19479..19808)
                     /gene="ygaM"
                     /locus_tag="ACHY09_002371"
     CDS             complement(19479..19808)
                     /gene="ygaM"
                     /locus_tag="ACHY09_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFS3885333.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            19960..20304
                     /gene="ygaC"
                     /locus_tag="ACHY09_002372"
     CDS             19960..20304
                     /gene="ygaC"
                     /locus_tag="ACHY09_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFS3885334.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(20341..20790)
                     /gene="alaE"
                     /locus_tag="ACHY09_002373"
     CDS             complement(20341..20790)
                     /gene="alaE"
                     /locus_tag="ACHY09_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFS3885335.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         5170 a         5322 c         5455 g         4927 t
ORIGIN      
        1 agcagtaaaa taaattttcc caatgcatcc gtagctcagc tggatagagt actcggctac
       61 gaaccgagcg gtcggaggtt cgaatcctcc cggatgcacc atattctccg taaccttcag
      121 caatgaaggt atcgaagagg tagcggtatt aaccgcaggc accagggagg ataacgttgc
      181 gccagcagcg gcccgaaggg cgaacgccag tgagtcatcc tcccggatgc accatcttct
      241 ccaaaaaatt tccttcaaaa atatctcatt tctaaataac tactttatcc atttcctgaa
      301 tattcttctc cttctccatc agcgacaaaa tcaagcaatt acgctaaagt agcgtcatat
      361 ttttcgactt gcgagaagac catgtacgag cgttatgcag gtttaatttt tgatatggat
      421 ggcacaatcc tggatacgga gcctacgcac cgtaaagcgt ggcgcgaagt attagggcac
      481 tacggtcttc agtacgatgt tcaggcgatg attgcgctta atggctcgcc cacctggcgt
      541 attgctcagg cgattattga gctgaatcag gccgatctcg acccgcatgc gttagcgcgt
      601 gaaaaaacag aagcagtaag aagtatgctg ctggatagtg tcgagccgct tcctcttgtt
      661 gaagtggtga aaagctggca tggtcgtcgc ccaatggctg taggaacggg gagtgaaagc
      721 gccatcgctg aggcattgct ggcacacctg ggattacgcc gttattttga cgccgtcgtc
      781 gctgccgatc acgtcaaaca ccataaaccc gcgccagaca catttttgtt gtgcgcgcag
      841 cgtatgggcg tgcaaccgac gcagtgtgtg gtcttcgaag atgccgattt cggtattcag
      901 gcggcccgtg cagcaggcat ggacgccgtg gatgttcgct tgctgtgagt gaagcgttat
      961 cgcttttctc gttgtttgcc agtagttttc tcagcgctac actattaccc ggcaactccg
     1021 aagtcgtgct ggtggcaatg ttgctttccg ggatcagtca tccctgggtt ttagttttaa
     1081 cagcaacaat gggtaatagc cttggagggt taactaacgt tatccttggg cgtttctttc
     1141 cattgcgtaa aacatcgcgc tggcaagaga aagctaccgg ctggctgaaa cgctatggtg
     1201 cagtcacgct attattaagc tggatgcccg tggttggcga tttactgtgt ctgttagcgg
     1261 gatggatgcg catctcgtgg ggaccggtaa tctttttttt gtgccttggt aaagcgttac
     1321 gctatgttgc ggttgcagca gctaccgttc agggcatgat gtggtggcac taattgtagg
     1381 cctgcacata tggtcaccat tacagttatg ctaattaaaa cgattttgac aggcgggagg
     1441 tcaatttgat cccggacgta tcacaggcgc tggcctggct ggaaaaacat cctcaggcgt
     1501 taaaggggat acagcgtggg ctggagcgcg aaactttgcg tgttaatgct gatggcacac
     1561 tggcaacaac aggtcatcct gaagcattag gttctgcact gacccataaa tggattacta
     1621 ccgattttgc ggaagcattg ctggaattta ttacaccagt ggatggtgat attgaacata
     1681 tgctgacctt tatgcgcgat ctgcatcgct atacggcgcg caatatgggc gatgagcgga
     1741 tgtggccgtt aagtatgcca tgctacatcg cagaaggtca ggacatcgaa ctggcacagt
     1801 acggcacttc taacaccggt cgctttaaaa cgctgtaccg tgaagggctg aaaaatcgct
     1861 acggcgcgct gatgcaaacc atttccggcg tgcactataa tttctctttg ccaatggcat
     1921 tctggcaagc gaagtgcggt gatatctcgg gcgctgatgc caaagagaaa atttctgcgg
     1981 gctatttccg cgttatccgc aattactatc gtttcggttg ggtcattcct tatctgtttg
     2041 gtgcgtctcc agcgatttgt tcttctttct tgcaaggaaa accaacgtcg ctgccgtttg
     2101 agaaaaccga gtgcggtatg tattacctgc cgtatgcgac ctctcttcgt ttgagcgatc
     2161 tcggctatac caataaatcg caaagcaatc ttggtatcac cttcaatgat ctctacgagt
     2221 acgtggcggg ccttaaacag gcaatcaaaa cgccatcgga agagtacgcg aagattggta
     2281 ttgagaaaga cggtaagcgg ctgcaaatca acagcaatgt gctgcagatt gaaaacgaac
     2341 tgtacgcgcc gattcgtcca aaacgcgtta cccgcagtgg tgagtcgcct tctgatgcgc
     2401 tgttacgtgg cggcattgaa tatattgaag tccgctcgct ggatatcaac ccgttctcgc
     2461 cgattggtgt agatgaacaa caggtgcgat tcctcgacct gtttatggtc tggtgtgcgc
     2521 tggctgacgc accggaaatg agcagtagcg aacttgcctg tacacgcgtt aactggaacc
     2581 gggtgatcct cgaaggtcgc aaaccgggcc tgacgctggg tatcggctgc gaaactgcgc
     2641 agttcccgtt accgcaggtg ggtaaagatc tgttccgcga tctgaaacgc gtcgcgcaaa
     2701 cgctggatag cattaacggc ggcgaagcgt atcagaaagt gtgtgatgaa ctggtcgcct
     2761 gcttcgataa tcccgatctg actttctctg cccgtatctt aaggtctatg attgatactg
     2821 gtattggcgg aacaggcaaa gcgtttgcag aagcctaccg taatctgctg cgtgaagagc
     2881 cgttggaaat tctgcgcgaa gaggatttta tagccgagcg cgaggcgtct gaacgccgtc
     2941 agcaggaaat ggaagccgct gataccgaac cgtttgcggt gtggctggaa aagcacgcct
     3001 gacagaaaag aaaaaggcca ctcgtgagtg gccaaaattt catctctgaa ttcagggatg
     3061 atgataacaa atgcgcgtct ttcatatact cagactcgcc tgggaagaaa gagttcagaa
     3121 aattttaaaa aaaattaccg gaggtggcta aatgccgttg ttagatagct tcacagtcga
     3181 tcatacccgg atggaagcgc ctgcagttcg ggtggcgaaa acaatgaaca ccccgcatgg
     3241 cgacgcaatc accgtgttcg atctgcgctt ctgcgtgccg aacaaagaag tgatgccaga
     3301 aagagggatc cataccctgg agcacctgtt tgctggtttt atgcgtaacc atcttaacgg
     3361 taatggtgta gagattatcg atatctcgcc aatgggctgc cgcaccggtt tttatatgag
     3421 tctgattggt acgccagatg agcagcgtgt tgctgatgcc tggaaagcgg caatggaaga
     3481 cgtgctgaaa gtgcaggatc agaatcagat cccggaactg aacgtctacc agtgtggcac
     3541 ttaccagatg cactcgttgc aggaagcgca ggatattgcg cgtagcattc tggaacgtga
     3601 cgtgcgcatc aacagcaacg aagaactggc gctgccgaaa gagaagttgc aggaactgca
     3661 catttagtca gtttaagcag aagaggccgg gctggtgaaa acaccgcccg gcttttgtta
     3721 cctgtcagtc atccgatatt ccacgctatg tgcttcatca ggttgaggaa cttccagcct
     3781 tgaggccatc cgcatcacga ccgcttcagg aactgcatac tcgcgtctgg cattttgctg
     3841 cttccattgc gcccatggca cttccagata aacgattttt actcgtgcgc cgtaagccgt
     3901 aaacagactg atgagctggc tgcgcagctg gcgggtaatg ttcgttgcat tccagatgaa
     3961 cggctttttc tggcgtaaaa aaacgcgcgc ctcttctttt gcctgttgca caatacgccc
     4021 ggtggcggtt ttgtcgtccg gactggcatt gattcgccgc cgcatatcat caaggctgat
     4081 aacgtccatt ccctggcact gttcactgat atagcgatct ttgcccatac ccggtaagcc
     4141 acacagcaaa ataacctcaa aaggttcagc ctcccaggga acaaaatcag gagaactttg
     4201 ctcatgagtc aggtagtgcc agcgtgcgga atcagaaacg aacgggcgca tttttcccca
     4261 gcattgttgc tcatggcaaa acagctcgaa cagatcgatg cgttccagca ttgattgctg
     4321 atccgggctt tggcgaccaa gcagatcggc acgagcaagc aaagcgagca gacgcgtgtc
     4381 gatgcgcatt gcagccgtga gcagcaaacg ctccggttcc ggacgttcca gtaaccatag
     4441 cggtaaccca tgcagacgca ccagcgcaac aatttgctcg cgtagcacga acggcgttgg
     4501 gatatcgcgc cataaaattt gtcgggccgt cagttcgccc cgacgagcat ggccaggcga
     4561 ctgaatacgt ccgttttcct gcacggtagt gctgcgcttt tcaacatcat gcaataacgc
     4621 cgccgcccat aaaacttcct gctgttgagc ggggagttgc tggaattctg gctgagtaac
     4681 gagggcgttc agcaccattt cggtatgcac gccgacgtca ccttcaccat gatgctccgg
     4741 gtcttgtggc gtgtgatgca tctcctcaac ccagctaaaa cgctgacgta gcgccgacca
     4801 gcgtttgtcg tcggtaagct gccaaatcat gactgttctc cataatcgaa ctcatgggcc
     4861 atccgcgccc gttgccagtg tcgtttccag tggacggtgg ttttgacatg atttttgcgc
     4921 acatacttga agacgttatg cgaaaagtca gcgacggaaa acgcatcacg attcctgctg
     4981 acaatgcctt ccagcgtaca gggttgcccg gtttgcgtat cccagggatc gaacgcgccg
     5041 cgcgcattgg tcagcgccag aaaatcacgt tgccagcttt tttcatcatt ccccggttgc
     5101 gggccggaga tttcgggaac gcaggggaaa tcaaacagag cggcgtaaaa ctgcacctct
     5161 tcccagctca gccacatatc ctggcagcga acggcaaaca gataaaaatc ctgctccagg
     5221 gcgcgatatt caatcgaatg cacggcataa agattttcac caaacaactc cagatcgccg
     5281 agatcgtttt tcagcagttg ccagcgttgg cgaattttat aggtccatgc cgactgcgta
     5341 ggcgcggcat gggaacgggc aaacacgccg tagcggttaa ggcagttatt ttcaccgtcg
     5401 agtttttctg tgtgcaccaa ttgcgtgatg gcctgtaaat cctgccagta atcggcatta
     5461 atgcggtcat cgctggtggt gccgggggag aaaggataat gccaggttct cccgtatttt
     5521 cgttgggtat tcataataat ccattatttt gtgtgtaata aatgtacgcc cgcgagggtg
     5581 cgagttatgg ggttaaaaga aaaatgtgag tatcacccgc gaaagcgagg gcgaaagaaa
     5641 aagagaatta acaacctgag cgaatgctca gaaccggtta atggtgaatg gaagagtttg
     5701 gggcggcgaa aagatacacc gaatgagtgg ataaaacaac cgtttgcaaa atgagcaaat
     5761 aaaaagggag ccgcagctcc ctttgacaac cttagtgcgc accgcctccg ccgccacctg
     5821 caccaaatgg cggtttagca aaccacacca gccccagcag gacgaggaat attccggctg
     5881 acatccagaa gatctcattg gcggaaataa tcagcccctg attagtgatc tgctgggcaa
     5941 tccagcctga tgcctgttgt tgcgtcatcc ccagcccttc cagttggctg tacatcgcct
     6001 gagcgttcgg gttaaacggg tttaccgact cggttaactg tgcgtggtgc atcgactcgc
     6061 ggttagtcca catggtggtg gtgatggatg tgccgataga ccccgccagc gttcgcgtaa
     6121 agttggagag gctcgatgcc gccgccagtc gttccggtgg caaaccggac agcgtaatgg
     6181 tggtcagcgg cataaagaag caggccaccg caaacccctg gataaactgc ggccaggccg
     6241 acgcgccaaa atccatacct ggttcaaagg tataggcgcg ccagtagaag cagacggcat
     6301 acataataaa gctgaaggtt accagccgcc gcatatccag tttatgcgcg aagcggccga
     6361 taatcggcga caggatcacc ggaataatcc ctaccggcgc agaggccaga cctgcccacg
     6421 ttgccgtgta accgtagacc tcctgcaaca actgcggcag cagaacaatc gcgccgaagt
     6481 agagcatata cgcgaggctg atacacaagc agccgatggt gaagttgcgc gacttaaaca
     6541 acgacagatc gactatcggg ttatcgtcgg tcagctccca gacaatcagg aagcagatag
     6601 ccaccactgc caccacggta aggatgataa tttcctgtga tgagaaccag tccagctctt
     6661 taccgcggtc gagcataatc tgcaggctac cgataccaat aaccagcagt gccagcccca
     6721 cggcatcaat ccgccgccgt tcggtgcggg tttcacgtcc gcgcagggtt tgcagtgtca
     6781 tcaacaccac cgccacgcca atcggtacgt tgatgaagaa tatccagccc cagtggtaat
     6841 tgtcactgat atacccgccg aggatcgggc cgcaaattgg cgcgacaatc accgtcatcg
     6901 accacaacgc cagcgcgatc gagcgtttgg ctggcgggta gttattaagc aatagacttt
     6961 gcgaaagcgg gatcagcggc ccggcgacaa tcccctgaat cacgcggaag aagatcagca
     7021 tattcaggct gctggagaca ccacacgccc acgacgcaat agcaaaggcg atggtggacc
     7081 agaggaacag tttcacttcc ccgacgcgct ttgccagcca gccggtaagc gggatcgaga
     7141 tggcattcgc caccccgaaa gaagtgatta cccacgttcc ctggctgagc gatgagccca
     7201 gattcccggc gatagtgggg atcgccacgt tagcaatggt ggagtccagc acctgcatga
     7261 atgtcgccag tgacagcgca atcgtcataa tgaccagttg cgcgccttcc agcggttttt
     7321 gctgttgcat cacacgcacc tctggattag ccagcgttag cttttacgat atcgtcgatc
     7381 agtttattga caggtgccag gctgatttca cgcgcggtgc ttaccgctac cggtgtggaa
     7441 cgtactttat ttgccagtac ctgaccgtca cggttagtgg tattgacgct caccagcgtg
     7501 gacaaaccga tacgcagcgg gtattgctcg agctgtttct ggtccagttc aatacgaaca
     7561 ggcagacgct gaacgacttt gatccagtta ccggttgcat tttgcgccgg aagcagtgag
     7621 aacgcgctac ctgtgcccat atccagacca accacttttc cggtgtattt cacatcatcg
     7681 ccgtaaatat cggtggtgat ggtgaccggc tgaccgatac gcatattggc aatctgcgtc
     7741 tctttaaagt tggcatccac ccacatattg gtggctggaa cgaccgccat cagcggcgtc
     7801 gttgggctaa tttgcgcccc aggctgtacc gcgcggcggg agacataacc ggtcatcgga
     7861 ctgacaatac gggtacgctc cagcgccagc caggcgttac gtacttcggt ggcagcctgt
     7921 tgcacggcag gctgatcttc cagtttagtc cccagaatca tcgcctgatt ggcattgtat
     7981 tgttgaatag cgacgtccag ttgtgcctgg gcactggtaa cggcgtcgcg ggcgtgttgc
     8041 agctcttcgc gaccaatcag gttggcattg cccagcggca cacggcggtt gtagtcgctt
     8101 tgtgctttcg cgagggcgat tttttgcacc tcaatattcg cctgcaactg tttgctgtta
     8161 atcatcagct ggtgggtttg gcgaacgctg gaagccagtg cagttttggc tttttcaaac
     8221 gcctggcgag catctgtcgg gtcgagagtg accagcacat cgccttcttt tacaaaatcg
     8281 gtgttatcgg cccagacttt cgtcacgctg ccagacacct gagacataat ttgaatctga
     8341 ttccctgcca cgtatgcgtc atcagtttct tcgaaatgac gcagtaccaa aaaccaataa
     8401 atccctatcg ctacggcaat aattataaag agcaaggtga gaaggaggag cagacgctta
     8461 cgtttgccgc tcttctttac cggttgctgc ggggtttgag tctccgcatt tgcgctcata
     8521 ttgttctcca cgatcttctt aactcatcgg ctgagccgac ctgcttgcca aaaaggccag
     8581 catgttgcga tgctggccag tcattttttc ttttataaat ctggattttt gagcgagatg
     8641 acgcgttagc tcatcgcttc gagaaccaca ccgtcttgtt ccatctggtc gagacgggag
     8701 agcaatttgc gggtgatttg ctcgagctga tctttttctg ttgtgctgag cgcggaccag
     8761 agttgatgca ggcagttatg ctgcggtggt aaaacctcgc gcaaaaactc gtgacctttt
     8821 tccgttaatt gcagatgcag gcagcggcga tcgttatcgc tttcacgacg ttcgatccaa
     8881 ccgcgttttt ccagttcatc ggcaatacgc gtcgcgttgg tacgggatga tccaagagca
     8941 caactcaatt cagaaggctg aatactgtgg ttttcctgag actccagcgt aatcaacgcc
     9001 ataaacaacg tctcgttaat cccctgagcc ttcagcattt tattgcggtt ctccagcagc
     9061 ttgctttgca tgtgcatgca aagacgggtc agaaggatct cctgataagg aaaatcttcg
     9121 tggcggctgg cgcgaaattt tagcatttgt tcaatgggcg taaacgaact atccatttgg
     9181 gtatgacctc attaatttca gccgatatag taacgacagt gacaaataaa gtaaatgtat
     9241 tgttttagaa aaatgattct tgtgggttat ataatcgcca tcactttcca ggcgagccca
     9301 taggccagcg cactaagcag tgttgggatg ataatgctgc gtgttttata gaaactggca
     9361 cccagtaccg cgaagccgac cagagtgggc acgaaacggc gtgtatcgtg catcacttct
     9421 ggtgcggtag agacaaccag cagagcgcat atcgaggcga tgccaatggt gtcgagcaaa
     9481 atacctaccg cgccacgttt ggttgggcgg gcattaccca cacgcaggcg cagcggcaaa
     9541 tagcggaagc aataattcgc cgcgccaacc agtaacccaa gcagcagaac ctcatagctc
     9601 atcgggcgct ccttgccaga atgcctggat taacgcagtg aggcagccac agacaatgcc
     9661 tgccagaatg gcgacgggaa tagaaaatag cgttacgcct gcaagggcac caactaacgc
     9721 tgcggtaacg caaagagatt gtttgcgctg gaaagaggcg agcaggaaac tcataaagag
     9781 tgccggaagc ataaaaccta atgctgcttc aacggcggga tatccttgca gcaagccgct
     9841 gccggagaat gcccctatta ccgtaccaaa tacccacgat gaccatgaac tgaaggcaat
     9901 gccgatcatc cagttctcgc tccagcggcg attattgcgt accagttttg cggttgcggc
     9961 ggcaaaaacc tcatccgtca ggccaaacgc ccacagggca gttttcgatt tttgcagacg
    10021 ctgaataata cggctacgca gtgacgggcc atacaaaaca tggcgaacat ccattgccat
    10081 gacggtcagt gcagcaaccc acaaactact cccggctgcc agcatcgcgg taatgacgaa
    10141 ctggctcgcg cctgcataaa tgatgcagga gaaaaaaacg ctttcgagag gagagaatcc
    10201 cagacgggtc gcattcagac cgaacgcaaa ggccaccgga atataactaa taacaatcgg
    10261 taaactgtct ttgcatcctt ccatgaaggt cgccgaacca ggagcaggct gtggagtagg
    10321 gctttccata gagtgtacgc ttaacgattg ttaatattat aaatagactg aatgaatatc
    10381 ttaaccttat cagactgatg ggcttcttaa cacccttata agtgtaaagc cacgaaaacg
    10441 gttgctgatt gcttaaattg cggcttcctg acgatgaaaa cctcgccacc agaccagtaa
    10501 gttaaccagg gcaatcgtcg cgccagccag acaaacgcca gcccaaccgc catgttgcca
    10561 gactgaggct gaaattagcg aaccggcggc accgccaatg aagtagctgg tcatgtaacc
    10621 tgcggtcagg cgattacgcg catcagggtg tattcgataa attaccgtct ggttagtgat
    10681 atgcacgccc tgcacggtga gatccagcac caggatgccg ataatcagcg ccagtacgga
    10741 agtgtgacca aaccagatcg ccagccatgg aagtaacagc agcagcagac cgaaagttgt
    10801 ggtgtggtgc gatttgccct tatcggcaaa tccgcccgcc ggacgagcgc ccaacgctcc
    10861 ggctgctccc gcaagtccaa acagaccaat gacgccatcg ctgtaattaa aaggcggagc
    10921 ggcgagcaaa aaggccatcg aggtccagag aatgctgaag ttggcaaagg tcaggcagcc
    10981 cagcaacgtg cgggtgcgca gaattttatc gctgataaac atgctgaaaa cggaaccaag
    11041 caactgtggg tagttgaggt gggtttctga tttcatttgt ggcagaccac gccataatgc
    11101 cagcgccatc agtgccatta acaccgaagc aacccaaaag acggtgcgcc agccgccgag
    11161 gtttgccagc aacccggcaa cggttcgtgc cagcaagatc cccaacagca gcccgctcat
    11221 aatggtgccg accactttgc cgcgtttgtc cggtgaagcc agcgtcgctg ccagcggaac
    11281 cagaatttgt gccacgaccg agaataaacc ggttaatgcc gtaccgagga tcatcatcgc
    11341 cagcgactga ctgctggcag tgattaacat gccgcatgcc gccagtaagg tcatcgagac
    11401 aatcaggcgg cggcgttcaa acatatcacc gaggggaaca agaaacagca gacctgcggc
    11461 atagcccaac tgcgcggcgg taacaataaa gcctgccgaa ctggcggaaa gggaaaagtt
    11521 acgcgcgatg gtgtcgagca atggctgggc gtaatagtta ctggctaccg ccaggccggt
    11581 ggcgatagac atcagcacga tcagcgccgg gctaagctca tgattaggtt tagtcattat
    11641 ttttgttcat caggtgatga ttaagctgga aatcaataat agcggatgtt gatgaatatg
    11701 ggatgaaaga tagattgttg aaatgtgcgg tgtggtttgt aggtatgata agacgcacca
    11761 gcgtcgcatc aggcattgtg tacagagcgg cggatgaggc aaaaacgcct tatccgcccg
    11821 aataaaaatt acttctgcgc tgccagcgcc tcattcaccc agccatcgaa ctgctgctgg
    11881 tgggctttga tccagccatc aacgtggccc tgaatatcgc cttctgaggc tttgccgtca
    11941 tgcataatgg cgttctgggc gttaatatca gccactggta actgcataat ggcaaacagt
    12001 ttcgctgctg ccgggttttt ctcggcccag gctttgttgg caacgatatg catggtactg
    12061 accgggaagc cgtaattcgc gccattcggc agtttggtat cggcattttt atcgcccggc
    12121 agtgcggaga acggcacctg caaccagacc acatctttcc ccggtttcag ttcgttactc
    12181 acccagtacg gcgtccaggt gtagtaaaac accggcttac cctctttgta acgactgatg
    12241 gtgtcagcca tcatcgctgc gtagttcccc tgattatgcg tcacggtatt ggtcagtcca
    12301 tacgcggcaa gctggtggtt gatcgcacct tcgcagcccc agcctgggtt acagccggtt
    12361 aaatccgctt taccgtcgcc gttggtatcg aacagtttgg cgatcttcgg atctttaagt
    12421 tgcgcgatgt tggtaatttt gtactggtcg gcagttttct tatcgatcag gtaaccctgt
    12481 gccgcgccgt taacaaatac tccttcacga taaaatttct tatcgccacc ggcagcttcg
    12541 tacatgttgt catgcagtgg cgtccagttc actgcggtga aggttgcatc gccggaagca
    12601 agcgaggtgt agccaacgtt gtaatccact tcgctgggtt tattgacggt ataacctaat
    12661 ttctccagcg cgcggctgac cagcagcgtc tggaaagttt cttcggtgat ggtgctctga
    12721 accggattaa cagtaatgcc tttgcccggc aggtcggcag caaaagtttg tgtagagata
    12781 agcgtggcaa acgctgtcgc aaaaagtacg ctatgtcgca ttgttattcc tttttttggc
    12841 agggtgataa tgcccggcgt caccgggcaa gtgcagagtt acttaatgaa tgggcgggtc
    12901 agcagaccga cagggccagt ggtgtaccag cgacggttgc cgcgactgcg tgagtcgcgc
    12961 ccaacggcct gcgtcaggcg gtcgaggata atggcgagga tcacaatccc gacgccgcca
    13021 acggtggcaa gccccatatc cagacgaccg ataccgcgaa gcaccatctg acccagcccg
    13081 ccgacggcaa tcatcgaggc gatgaccacc atagaaaggg ccagcatcag cgtctggtta
    13141 acgcccgcca taatggtcgg catcgccagc ggtaactgaa ctttgaacag catctggcgc
    13201 gggctggcac cgaatgagcg cgaggcttca atcagatccg ccggaacctg gttaattccc
    13261 agaatcgtca gacggataat cggcggcagc gcaaagatga tcgtcaccac cacgcccggc
    13321 acgttaccga taccaaacag catgacgatt ggtaccagat aaacaaacgc gggcgtggtc
    13381 tgcatggcat caagcagtgg acgaataatt ttcgccgctc gcggacttct cgccagccag
    13441 atccccaacg gcaaaccgat gacgatacag aacagcaggg cggttagcac cagcgccaga
    13501 gtaaccattg cctgtgacca ggcaccgatt gcgccgatgg caatcagcga aaccagtgtc
    13561 gccacgccca ttccgacacc ggaaatttgc caggcgatga gagcgaaaac gataatcgcc
    13621 accggagcgg gcatacccag cagcaattgc tggaaaccgt tgaggatgta atcaaccgga
    13681 acgcgcacgc cctgaaagac gggacgaaaa tgggtaacga cccagtcgat cccttcagtg
    13741 acccaactgt cgagcgggat cagcgtttta tggaacggat cgagaatatt aaaatgctcg
    13801 acgtttggcg caggcgtact ggtcagccag tcagcaccac cgccgtcagt cggtgcagca
    13861 gccggtgtac cccaggcgtc tgcggactgt gcggcactgt ccgccgctgg cgtggtatcc
    13921 cacggattat tttgatcagc cattatttac cccctcacga tctaaagcgc gcagcagcat
    13981 tcctttcgaa atgatgccga catactgttg gtcctcatcg accacgggca ccgcacaggg
    14041 ggcctgtccg acatgagaga gcaactcgct aagaggcgtt tgtgcatcga ccgctaacgg
    14101 cgcatcaatc agcgccgcat caagaccttg ctgctgcgtt aacgcggctt taagcgaatc
    14161 gatagacaca gcgccgacaa acttattacc gcgttcgatg acgtagccat attcgcgatc
    14221 ttcatcctgc aataatttca gtgccgaacg cgggccgaag ccgggggttt tacgaattaa
    14281 gccgttcggt gtacgacggg caatatcttt cgcactgaac acctggctaa tatcgacgcc
    14341 acggaagaag gtacggacat aatcattcgc cggattattg agaatttcat ccggtgtgcc
    14401 gacctgtact acttcaccat tttgcataat ggcaattcgg tcgccaatac gcatggcttc
    14461 atcgagatcg tgggaaataa agacaatggt gcgctgatgt ttcgcctgta attttaccag
    14521 ctcatcctgc atctcggtgc gaattaatgg atcgagcgcc gagaaggctt cgtccattaa
    14581 taatatatcc ggattaatcg ctaacgcgcg ggctaatccc acacgttgac gcatcccgcc
    14641 agagagttca tccgggtagc tgtgggcata attttccagc ccgacctgac gcagtgcatc
    14701 aagggctttt tcacggcgtt cttcggcatt aattccggcc agttccatac cgaacgcagt
    14761 attgtccagc acggtcatat gcggcattaa ggcaaaggac tggaagacca tcgcaatctt
    14821 ttttctgcgc acctcacgga gttcggcgtc ggatattttg gcaatatcca caccatcaat
    14881 cagcacttgc ccgcgggtgg gttcaatcag gcgattgaga aggcgtacca ttgtggattt
    14941 acccgaaccg gataatccca tgatgacaaa tatctcgcct tcttcaatgg ccagactggc
    15001 gtctttaacg ccaagcgata gcccagtttt ttccagaatt tgttcttttg aaagtccttg
    15061 ttcgatatat ttgaaagctc gctgtggatg ctcgccaaat attttataaa gatttttaat
    15121 ttctaattta attgccatgc aatagaaaga ttcctgttat ttgtttatgt cgatacatta
    15181 cccaatggaa atagtcactt ttttctaccc taacatactg agaatctgag gcaacccctg
    15241 atggcaaatg tggcatgaat attgatgtga gcaaatcgcc ggaatttccc atgatataag
    15301 ggctgagagc aaatcgagaa aagttgatat tttttgtcaa agatagcaaa aaaacgcctg
    15361 aatattgtgg tttcaggcga ataatgcaac agagtatcag tgtagatcac cacaaattat
    15421 ttgcgtgata taaagctatt tggcgggaat tatttccctg cagcgggtag tgatattttt
    15481 gaaaataacc ccctcagaaa ttccagtctt catcttctgt ttcgaccgct tttcccatca
    15541 catatgaaga gcctgaaccg gaaaagaaat cgtgattttc atcggcattc ggcgaaagcg
    15601 cggcgaggat tgccggattc acttccgcca tttctgcggg aaatagcggc tcgtagccca
    15661 gattcatcaa cgccttattg gcgttgtaac agagaaacgc tttcacatcg tcagcccacg
    15721 gggtttcggc gtacagctca tcggtgtatt gcaactcgtt gtcatagagt tccagcaaca
    15781 aatcgaaggc gaaactcttc aattcttcac gtcgtgcctg ggagatcttt tccaggtttt
    15841 tctgatattt atagccgatg tagtaaccgt ggactgcttc atcgcggata atcagacgga
    15901 tcaggtctgc ggtattggtt agctttccgc ggctggaaaa atacatcggc agccagaaac
    15961 cggaatagaa caaaaaagat tcaagaaaca cactggcgat tttctttttc agcggatcat
    16021 caccgcgata atgttgctga ataatctgtg cttttcgctg caacggcgcg ttttcttcac
    16081 tccaggcgta ggcggcatcg acatctttgg tctggcacag cgtcgagaaa atcgaactgt
    16141 aagagcgggc atgaaccgcc tccataaagc tgatattcga taatactgcc tcttcatgag
    16201 gcgtgagcgc atcgggcatc agcgaaggcg cgccgataac attttgcagc gtgtcgagca
    16261 gtgtcaggcc agtaaaaacg cgcatcgtca gttgttgttc tacgacactt aatgtctgcc
    16321 aggcgggaat atcgttcgat agtggcacct tttctggtag ccagaaattg ctggtcaggc
    16381 ggttccacac ctccagatct ttatcgtctg ttatcttgtt ccagttgatg gcgctgatac
    16441 gtgagagttt catagatatt ccttaaagtg cacaggagac gcagccttca atttcagtgc
    16501 cttccagcgc catctgacgc aggcggatgt agtagagcgt tttgatacct ttgcgccagg
    16561 cgtaaatctg cgctttgttg atatcgcgag ttgtggcggt atcggggaaa aacagtgtca
    16621 gcgacagccc ctgatcgaca tggcgagtcg cttcggcgta ggtgtcgatg atcttttctg
    16681 cgcctatttc gtaagcgtcc tgatacagcg ccagattctc gttagtcata aacggggcag
    16741 gatagtaaac gcgtcctgtt ttgccctctt tgcgtatctc tactttcgcc acaatcggat
    16801 gaatactcga cgtggcatgg ttgatataag agatagaacc ggttggcggc accgcctgga
    16861 gattttggtt gtatatgcca tagcgcatca cgtcgtcgcg cagttgcgcc cacatttcgc
    16921 gggtaggtaa cgtgatgctg ctacgagcaa acaactcgcc aactttcgcc gttttcggtt
    16981 gccagttact ttgcagatat tggctaaaat attcaccgct ggcatagcgc gactgtttga
    17041 acccggcaaa ggtttcgccg cgttcgcgcg ccagcaacat cgaggtacgc agtgcgtgcc
    17101 aggtgatggt atagaaatag agattggtga aatcgagtgc ttccggcgat ccataagcga
    17161 tgccttctcg cgccagataa ccgtgtaaat tcatctgccc cagtccgatg gcgtgtgagg
    17221 cggcatttcc ggcttcgatg gacggcacgc tgcggatatg actcatatct gacaccgccg
    17281 ttaaaccgcg cacggcagtc tctaccgtgc gggcaaagtc gggggaatcc atggtgtggg
    17341 caatattcaa cgaacctaaa ttgcaggaaa tatcatggcc tgtgcgggca tagtcgagat
    17401 tctcgtcata ctctgaggcg ctgttaacct gcaaaatttc tgagcagaga ttgctcatat
    17461 ttatgcgccc ggcgataggg ttagcacggt taaccgtgtc ttcatacatg atgtacggat
    17521 agccggactc aaactggatt tctgccagtc gctggaagaa atcacgggcg ttgatgtatt
    17581 ttttgcggat gcgttcattg gcaaccagtt cggcatagtg ttcactgatg gcgacatcgg
    17641 caaatggctt gccataaact cgctctatgt catagggcga aaacagcgcc atctgcgcat
    17701 tctcttttgc cagatggaaa gtgatatccg ggatcaccac gcccagcgac agtgttttaa
    17761 tgcggatttt ttcgtcggca ttttcccgtt ttgtatcgag aaaacgcaga atatcgggat
    17821 gatgagcatg taaatagact gcgccagcac cctgacgcgc gccgagctgg ttggcatagg
    17881 aaaatgcgtc ttccagcatt ttcatcaccg gaatcacgcc agaagattga ttttcaatac
    17941 gtttaattgg cgcgcccgct tcccgcagat tcgacagcaa aaatgcaacg ccgccgccgc
    18001 gtttcgacag ttgcagtgcg gaatttactg cccggccaat cgactccata ttgtcttcaa
    18061 tacgcagcaa aaaacaggaa accagttcgc cgcgctgctg cttaccacag ttgaggaatg
    18121 tcggtgttgc aggctgaaag cgtcctgaca gcatctcatc ggtcagttgc gtcgccagtg
    18181 tttcatcgcc ttgtgccagc gtcagcgcca ccatcgtgac tcgatcggca aaatcttcca
    18241 gataacgctt accgtcgaat gtcttcaacg tatagctggt gtaaaacttc catgctccga
    18301 ggaatgtctg aaaacgaaaa ccgctggcgt gtgcgtgggc aaacagcgta atgacaaaat
    18361 cgctagagta gcgattaagg acactttcat catagtaacc ttcgttaacc agccagttca
    18421 ggcgctgctg ctggctactg aaggccacac tgttcggacg cacatgcgtc gtcataaagg
    18481 cgtctactgc ctggcgatct ttatcgaact gaatgcgacc tgcgctatcg taaaggttaa
    18541 gcatcgcatt cagcgcgtgg taatccatag tttcctgcgt caggcgttct gcggttgtcg
    18601 ttgccaaaat tcggttactc ctttacgaac gttttcgata tcgctttgcg tacccatgag
    18661 ttcaaagcgg tacagccatg gcacgccgca tttccgggca atcacatctc cggcgcggcc
    18721 atatgcctca ccaaagttgc gattacccga agcaataacg ccgcgcagca acgcccggtt
    18781 atgctcgtcg tttaaaaagc gaatgacctg tcgtggcacc gcgccagccg taccgccgcc
    18841 gccgtaagag ggcacgatca ggatgtaagg ctcgtctacc tgaatccgtt cccgctcatt
    18901 gagcgggatg cgcaccgcgg gcagccctaa acgttcgata aaacgctgcg tattttcgga
    18961 gctgctggaa aagtagacaa tctggctcat gcattggccg cgtgtggtgc tggatgcaga
    19021 cggttgatca tgtccgggcg gaaacctgac cagctaagat cgcctgcaat cacgaccggc
    19081 aactgacgaa agccctgagc acgcagcgct tccgccgctt caggaacgcg atcgacatta
    19141 atcatttcaa agtcaaagcc ccggttttcc atcgcccttt tggtggcgtg gcactgaacg
    19201 caatcgttac gagtgtaaat agtaatgcgc atgattcgta tttccgttta aaatgaagat
    19261 acggcgcgat gatacgcgtc gggttgtctc tctgttgata cagaaatact agatgtagtt
    19321 gaaaaaagat tcaaccacac aatatatagc aaatagtggt cgaaattacc ctgggtatga
    19381 gcgtgacggg gtagggggat ttttgtgatt caccaggcaa aaagaaaccc cgaagccagg
    19441 cttcggggtt aaagacgcgt atttattatc atgttgcact acgatttgcg catgcttaac
    19501 agtgcgccga taaaaatacc taccgcagct gctgtaccca cgctacacca gggtctttca
    19561 cgaacaaaag aatccgcgca gccaacggca tcgcgcgcgg cttgctggac gcgagtacga
    19621 ccatgcattc gcgctctggt ttctttcagt agagcctgcg ctttactacg agcggcttcg
    19681 gcttcccctt tggcgtcgct gccccaggac ttcaatacag attcgagact atcagctaat
    19741 tgattgacat cattctggat atcctgcacg ccatcatcaa cgtcgttgcg gttcggtcgg
    19801 ttaaacatat gatcccccat tagaattcga tgtaattttc agtgtagacc ataattttct
    19861 taactgaggt tcatcgcagc tttatggact tttctgaaag cgttgtgaat gcgcaatcag
    19921 gtaaggtagg cccgcggttg ataataacga ggatcaaata tgtatttacg accagacgag
    19981 gtggcgcgcg tacttgaaaa agtcggtttt actgtcgatg tggtaaccca gaaggcttat
    20041 ggttaccgcc gtggggaaaa ttatgtctat gttaatcgcg aagcgcgaat gggacgtacc
    20101 gccctggtga ttcatccaac attaaaagaa cgtagttcaa cactggctga acccgcttcc
    20161 gatattaaaa cttgcgatca ttatcaacaa tttccgctct atttagcagg cgagcgacac
    20221 gagcattacg gtatcccgca tggctttagt tcgcgtgttg cgcttgaacg ttatttgaat
    20281 ggtttatttg gcgaagccag ttaaagacgc gactggcgat gccagtcgcg aaaagaaggg
    20341 tcaggctttt acctgctggt aacggctgac tttaaacagt cggcggcaat aatcgaggaa
    20401 gtagccataa accgccccca tcaacatcga aacaacgatg tttgaactga ccgccgccat
    20461 aatctgatgc cagtctgcgc ccaccactaa caagatcgcc acatacaccg gtgactggaa
    20521 cgtcacataa gccatgatat ccgccagatt ttttatccag cccgacgggc taactttgcg
    20581 tgctgcgcgc ataaacagat cgcggtacat accgtatggc catgcaatta agatgtttac
    20641 cggaatcgct accaatctgg aataaaaaga ctgttcgaag ctcattccgg agaggaaaac
    20701 ttcaatacac atgttcacga cagaacagta aacaaccatc gcgaacgtat ctgcaactgc
    20761 atgacgcaag cgtgactgcg gtgagaacat gctaatgctc cttaataaaa gcgtaatggg
    20821 atgtgaatgg agatgaaagc agtggatctc attgccatct gcatttttgc atag
//
LOCUS       DAZUCC010000075        20656 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZUCC010000075 DAZUCC010000000
VERSION     DAZUCC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.075"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..880)
                     /gene="ygbI"
                     /locus_tag="ACHY09_002374"
     CDS             complement(113..880)
                     /gene="ygbI"
                     /locus_tag="ACHY09_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFS3885336.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            1076..1984
                     /gene="ltnD"
                     /locus_tag="ACHY09_002375"
     CDS             1076..1984
                     /gene="ltnD"
                     /locus_tag="ACHY09_002375"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFS3885337.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1981..3243
                     /gene="otnK"
                     /locus_tag="ACHY09_002376"
     CDS             1981..3243
                     /gene="otnK"
                     /locus_tag="ACHY09_002376"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFS3885338.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSSAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3240..3878
                     /locus_tag="ACHY09_002377"
     CDS             3240..3878
                     /locus_tag="ACHY09_002377"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFS3885339.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            3883..4659
                     /locus_tag="ACHY09_002378"
     CDS             3883..4659
                     /locus_tag="ACHY09_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFS3885340.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLAQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADHFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4748..6112
                     /locus_tag="ACHY09_002379"
     CDS             4748..6112
                     /locus_tag="ACHY09_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFS3885341.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCI
                     WAVI"
     gene            complement(6150..6386)
                     /locus_tag="ACHY09_002380"
     CDS             complement(6150..6386)
                     /locus_tag="ACHY09_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFS3885342.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWMVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(6397..7824)
                     /locus_tag="ACHY09_002381"
     CDS             complement(6397..7824)
                     /locus_tag="ACHY09_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFS3885343.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFNILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARQ"
     gene            complement(7824..8417)
                     /locus_tag="ACHY09_002382"
     CDS             complement(7824..8417)
                     /locus_tag="ACHY09_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFS3885344.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            8564..8971
                     /locus_tag="ACHY09_002383"
     CDS             8564..8971
                     /locus_tag="ACHY09_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS3885345.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMSK"
     gene            complement(9092..10084)
                     /gene="rpoS"
                     /locus_tag="ACHY09_002384"
     CDS             complement(9092..10084)
                     /gene="rpoS"
                     /locus_tag="ACHY09_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFS3885346.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(10147..11241)
                     /gene="nlpD"
                     /locus_tag="ACHY09_002385"
     CDS             complement(10147..11241)
                     /gene="nlpD"
                     /locus_tag="ACHY09_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFS3885347.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(11426..12052)
                     /gene="pcm"
                     /locus_tag="ACHY09_002386"
     CDS             complement(11426..12052)
                     /gene="pcm"
                     /locus_tag="ACHY09_002386"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFS3885348.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(12046..12807)
                     /gene="surE"
                     /locus_tag="ACHY09_002387"
     CDS             complement(12046..12807)
                     /gene="surE"
                     /locus_tag="ACHY09_002387"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFS3885349.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(12788..13837)
                     /gene="truD"
                     /locus_tag="ACHY09_002388"
     CDS             complement(12788..13837)
                     /gene="truD"
                     /locus_tag="ACHY09_002388"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFS3885350.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQKLSWNWWDDVTVEIRFCLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(13834..14313)
                     /gene="ispF"
                     /locus_tag="ACHY09_002389"
     CDS             complement(13834..14313)
                     /gene="ispF"
                     /locus_tag="ACHY09_002389"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFS3885351.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(14313..15023)
                     /gene="ispD"
                     /locus_tag="ACHY09_002390"
     CDS             complement(14313..15023)
                     /gene="ispD"
                     /locus_tag="ACHY09_002390"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFS3885352.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(15042..15353)
                     /gene="ftsB"
                     /locus_tag="ACHY09_002391"
     CDS             complement(15042..15353)
                     /gene="ftsB"
                     /locus_tag="ACHY09_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFS3885353.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(15546..15869)
                     /locus_tag="ACHY09_002392"
     CDS             complement(15546..15869)
                     /locus_tag="ACHY09_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFS3885354.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(15919..16524)
                     /gene="cysC"
                     /locus_tag="ACHY09_002393"
     CDS             complement(15919..16524)
                     /gene="cysC"
                     /locus_tag="ACHY09_002393"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFS3885355.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(16524..17951)
                     /gene="cysN"
                     /locus_tag="ACHY09_002394"
     CDS             complement(16524..17951)
                     /gene="cysN"
                     /locus_tag="ACHY09_002394"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFS3885356.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(17953..18861)
                     /gene="cysD"
                     /locus_tag="ACHY09_002395"
     CDS             complement(17953..18861)
                     /gene="cysD"
                     /locus_tag="ACHY09_002395"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFS3885357.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            19113..20150
                     /gene="iap"
                     /locus_tag="ACHY09_002396"
     CDS             19113..20150
                     /gene="iap"
                     /locus_tag="ACHY09_002396"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFS3885358.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT         4785 a         5568 c         5325 g         4978 t
ORIGIN      
        1 agatttatat tgataccgga tcgccgaaaa gcgggcggct gtcattttat aaaataaagt
       61 agtctcatgc ttcttctgtg aagcatgagt aacccggcgt tattgcaggt cattattcat
      121 ttttcgctac cagcaaagag agatccagct tcgccagcgc gcggctggca ctctccggca
      181 gaccatcgtc agtaatgatc tgatcaaact cgcttaatgg taacgccagc catgtcgcca
      241 cctgtccata tttcgtcgca tcacagacca aaactcgctg gcggctggca ctggcaatcg
      301 cccgtttcac cgtgacttta tcttctgctg gcgtagagat cccccgcaca ctccatgacg
      361 atgcagaaat aaaagcctga tcaatcatca ggctacgcag catggtcgca gcggcttccc
      421 caacacagga acggttttcc cgacacactg cgccgccagt gtgaataatt gtgcaattac
      481 tgttgtcgag caagtagtcc gcaataacga agtcgttagt aaccactgtg agtggctcca
      541 tgtgagtcag atgctgtgca atcgctaacg tggtcgttcc cgcatccaga tagatacaac
      601 gtcccggctg aacaagactt gccgccagct tgccaatagc cgctttttgc gtcattgcca
      661 gcgcagtttt tacctgatga gaaggttcat gcgccacccg tcccggagac tggacgcccc
      721 cggacaccaa cacaacggct ccctgctgct ccagtttttg taaatcccga cgaatggtca
      781 tatgtgacac attcattctg tccgttagtt cagcaatact aacaatgcct ttttcagcta
      841 ccatctcaag gatgatttgg cgacgctcta cgggtatcaa cttttgctcc ttcctttgtc
      901 ctgctgacat tctacgctat ttgcctgcga aacgtgcgcg gcgcaactaa cgcttagttc
      961 acataaaata acatacaatg ttaatttttg tgaatcagct caccatatcg ttatctttcc
     1021 gcgcttatat tcacaacatc aaacaaaata tcacttaaat taacaaggag agcagatgaa
     1081 aacgggatcg gagtttcatg tcggtatcgt tggcttaggg tcaatgggaa tgggagcagc
     1141 actgtcatgt gtccgcgcag gtctttctac ctggggcgca gacctgaaca gcaatgcctg
     1201 cgctacgttg aaagaggcag gtgcttgcgg agtttctgat aacgccgcga cgtttgccga
     1261 aaaactggac gctctgctgg tgctggtggt caatgcggcc caggttaaac aggtgctgtt
     1321 tggtgaaaca ggcgttgcac aacatctgaa acccggtacg gcagtaatgg tttcttccac
     1381 tatcgccagt gctgatgcgc aagaaattgc taccgctctg gctggattcg atctggaaat
     1441 gctggatgcg ccagtttctg gtggtgcagt aaaagccgct aacggtgaaa tgactgtcat
     1501 ggcctccggt agcgatattg cctttgaacg actagcaccc gtgctggaag ccgttgccgg
     1561 aaaagtttat cgcataggtg cagaaccggg actaggttcg accgtaaaaa ttattcacca
     1621 gttgttagcg ggcgtacata ttgctgccgg agccgaagcg atggcacttg cagcccgtgc
     1681 ggggatcccg ctggatgtga tgtatgacgt cgtgaccaat gccgccggaa attcatggat
     1741 gttcgaaaac cggatgcgtc atgtggtgga tggcgattac accccgcatt cagccgtcga
     1801 tatttttgtt aaggatcttg gtctggttgc cgataccgcc aaagccctgc acttcccgct
     1861 gccattggcc tcaacagcat tgaatatgtt caccagcgcc agtaacgcgg gttacgggaa
     1921 agaagacgat agcgcagtta tcaagatttt ctctggcatc accctaccgg gagcgaaatc
     1981 atgatcaaga ttggcgttat cgccgatgat tttaccggcg cgacggatat cgccagtttt
     2041 ctggtggaaa acggtctacc aacggtacaa attaacggtg ttccaacagg taaaatgccg
     2101 gaagcaatcg acgcactggt gatcagcctg aaaacgcgct cctgtccggt ggttgaagcc
     2161 acacagcaat cgctggcggc tctgagctgg ttgcaacagc aaggttgcaa acagatctat
     2221 ttcaaatact gctctacttt cgacagttcg gcgaaaggta atatcggccc ggttaccgat
     2281 gcattaatgg atgctctcga cacgccgttt acggtcttct ctccggccct gccggtcaac
     2341 ggacgtacgg tttatcaggg gtatttgttc gtaatgaatc aactgctggc cgaatccggg
     2401 atgcgccatc acccggtaaa tcccatgacc gacagctatc ttccccgtct ggttgaagcg
     2461 caatccacag ggcgctgcgg cgtcgtttcg gcacatgttt tcgaacaagg tgtggatgcc
     2521 gttcgtcagg agctggctcg cttacagcaa gagggctacc gctacgcggt gcttgatgcg
     2581 ctgaccgaac accatctgga aattcaggga gaagccttgc gcgatacccc actggtaacg
     2641 ggcggttctg gtctggcgat tggccttgcc cggcagtggg cgcaagaaaa cggtaaccag
     2701 gctcgcgaag cagggcgtcc gctcgctggg cgcggcgtag tgctctccgg ttcatgctct
     2761 caaatgacca accgccaggt ggcacattac cgtcaaattg caccagcccg tgaggttgat
     2821 gtggcacgct gcctctcaac cgaaactctg gccgcttatg cacacgagct ggcagagtgg
     2881 gttctgggcc aggaaagtgt acttgctcca ctggtttttg ccaccgccag cactgacgca
     2941 ttggcagcaa ttcaacagca atacggtgca caaaaagcca gtcaggcagt ggaaacactg
     3001 ttttctcaac tagcggcgcg gttagcagcg gaaggcgtga cacgctttat tgtcgcaggc
     3061 ggtgagacct ccggcgtagt cacacagagc ctgggcataa aaggttttca tattggccca
     3121 accatttccc ccggcgtgcc gtgggtaaac gcactggata agcctgtctc actcgccctt
     3181 aaatctggca acttcggtga tgaagccttt ttttcacgag cccaaagaga gtttttatca
     3241 tgagcgattt cgcaaaagta gagcagtctt tgcgagagga gatgacgcgg attgccagtt
     3301 cattctttca gcgcggatat gcaactggtt cggctggcaa tctgtcgctg cttttacctg
     3361 acgggaattt actggcgaca ccgacaggtt catgcctggg caatctcgat ccgcagcggc
     3421 tttccaaagt caccgcggat ggcgaatggt taagtggtga caaaccctcg aaagaggtgc
     3481 tctttcatct ggcgctgtat cgcaacaatc cgcgctgtaa agcggtagtg catttgcaca
     3541 gtacgtggtc gacggcgctt tcctgcctgg aagggctgga cagcaataac gttattcggc
     3601 cgttcacacc atacgtggtg atgcggatgg gaaacgtccc gctggtgccc tattaccgac
     3661 cgggcgataa acgcatcgca caggatctgg cggaactggc agcagacaat caggcttttt
     3721 tactggcaaa tcatggccca gtggtttgcg gtgaaagcct gcaagaagcc gccaacaata
     3781 tggaagagct agaggaaacg gcaaagctga tttttattct cggtgaccgc ccgatccgtt
     3841 atctgaccgc aggtgaaatt gcggaattaa ggagttaatg caatgcctcg ttttgcagct
     3901 aatttatcca tgatgttcac cgaagtgcct tttattgaac gcttcgccgc agcgcgaaaa
     3961 gccgggttcg atgctgtgga gtttctgttt ccctatgact actcccccca gcaaatccag
     4021 cagcaactgg cgcaaaacca cctgacgctg gcgctgttca ataccgctcc cggagatatt
     4081 aatgcgggag aatggggatt atccgccctt cccggacgtg agcacgaagc acgtgcagac
     4141 atcgacctgg cactggaata tgcattggcg ctcaattgtg aacaagtcca tgtgatggca
     4201 ggcgtcgtgc ccgctggcga agatgctgaa cggtaccggg cagtatttat tgataatctc
     4261 cgctacgctg ccgaccactt tgcgccacac ggcaagcgaa tattggttga agcactcagc
     4321 cccggcgtta agcctcatta tctcttctcc agccagtatc aggcactggc tattgttgaa
     4381 gaggttgcgc gagataacgt ttttattcaa ctagatactt tccatgcgca aaaagtagat
     4441 ggcaacctga cgcatttaat ccgcgactac gccgggaaat atgcgcacgt acaaattgcc
     4501 ggactacctg atcgacatga accggacgat ggagaaatca actatccgtg gctgttccgc
     4561 ctgttcgatg aagtgggata tcagggctgg atcggttgtg aatacaaacc tcgtggcctc
     4621 accgaagaag gacttggctg gtttgacgcc tggcgctaat tcgttaaatc cctaattaca
     4681 acgtacccat acatcccccc atctacggat ggggtaggtt aactataact ttcagacagg
     4741 gtttcccatg tccacaatta cattgttatg cattgcgtta actggcgtaa tcatgctgtt
     4801 gctgctggtc atcaaggcaa aggtacaacc attcgttgct ctgctcctcg tcagcctgtt
     4861 agtcgcactt gcggcgggta taccggcggg cgaagtgggt aaagtgatga tcgccgggat
     4921 gggcggtgtg cttggttccg tcactattat tattggtctg ggcgctatgc tggggcgtat
     4981 gatcgaacac tctggtggtg cagagtcact ggctaattat ttcagtcgca agttaggcga
     5041 caaacgaact atcgctgcgc tgactctggc agcgttcttc ctcggtattc ccgtcttctt
     5101 tgatgtcggc tttattattc ttgcgccaat catttacggt tttgccaagg ttgccaaaat
     5161 atcaccactc aaatttggtc tgcctgtcgc tgggatcatg ctcactgttc acgtggcggt
     5221 accgcctcat ccaggccctg tcgccgcagc ggggttactc cacgcagaca tcggctggct
     5281 aaccatcatc ggtattgcga tttctattcc cgtagggatt gttggctact ttgcagcgaa
     5341 aataatcaat aagcgccaat atgcgatgtc ggtcgaggta ctggaacaga tgcaactggc
     5401 tccggccagt gaggaaggcg cgacaaaatt aagcgataaa ataaatccac cgggcgtcgc
     5461 gctggtcacc tcgctaattg ttattcctat cgcgattatc atggcgggta cggtttccgc
     5521 aacactgatg ccgccttcgc atcccctgct tggtacgcta cagctgatcg gctcaccaat
     5581 ggtagctcta atgattgcgc tggtgctggc attctggtta ttggctttac gtcgcggctg
     5641 gagcttacaa cacaccagcg acattatggg ctcagccctt cccactgcgg cggtagtgat
     5701 tttggttact ggtgctggag gggtatttgg caaagtgctg gtggaatcgg gcgtcggtaa
     5761 agcccttgcc aacatgttac aaatgattga cctgcctctg ttaccagccg catttattat
     5821 ttcactggcg ctgcgtgcat cgcaggggtc agcaaccgta gcaatcctga caaccggcgg
     5881 gttactctca gaagcggtga tgggattgaa tccaattcag tgcgtattgg tgacgctggc
     5941 agcctgcttt ggtgggcttg gcgcgtcaca tattaatgac tcagggttct ggattgtgac
     6001 caaatatctg gggttgtcgg tagcagacgg gctgaaaacc tggacagtgt taacgaccat
     6061 tctcggtttt accggattct taattacctg gtgcatatgg gcggtaattt gaccttaata
     6121 aaaggtccga tgggcatcgg accttttatt tagcgcttac cttccgccag cagcggcggg
     6181 atgctcggca ccatcggcgc gtcatcaata tctttctgcg tcatgcggaa cgcttcggga
     6241 taatgttcgc ggctggtgcg gcgtagcggt tcggtatcgc gccaggtata gaggcaatgc
     6301 tggcactggt agaccatcca gacatctttt accggcgatt tcgccatcac ttcaatctgt
     6361 tcatcggcac aacgtggaca aatcatcttg ttctccttat tgacgtgcgg ccagcatagc
     6421 ggtcagtttt tcagcccagg ctttggtttc cggtaagtcc accaccggct ggctgtagtg
     6481 accacggttg tccggggcga caggcgtggt ggcgtcgata atcagcttgt cggtgatccc
     6541 cgccggactt gagccagggt cgagttccag cacggacata ttcggcaact gtaccaaatc
     6601 ccctgccggg ttcactttcg aggagagcgc ccacatcacc tgcggcaggt tgaacggatc
     6661 aacgtcttca tcgaccataa tcaccatctt cacgtagccc agaccgtgcg gcgtggtcat
     6721 cgcacgcagg cccaccgcgc gggcaaagcc gccgtagcgt tttttggtgg agataatcgc
     6781 cagcaggccg tgggtgtaca tggcgtttac cgcctgcact tccgggaact cggctttcag
     6841 ttgttgatac agcggcacgc aggtggcagg ccccatcagg tagtcgattt cggtccacgg
     6901 cataccaaga tagagcgatt cgaaaatcgg tttggtacgg taagagactt tatcgatacg
     6961 caccacggtc atgttacgcc cgccggagta gtggccggta aactcaccga acggcccttc
     7021 aatctcacgt ttacgacttt cgataacccc ttcgaggatc acttccgagc cccacggcac
     7081 atcaaaaccg gtcagcgggg cggtggcgat cgggtacggg ctttcgcgca gcgcgcctgc
     7141 catttcgtac tcagactgat cgtatttcag cggcgtggcc cccattaggg tgatgatcgg
     7201 atcgttaccg agtgtgatgg caatcggcag atcttcaccg cgctcttctg ctttatgcag
     7261 atgcagggcg atatcgtgca tcggcaccgg ttgcaggccg agcttacgct tgcccttcac
     7321 ttccatgcgg tagatgccga cgttctgctt gccgaagtta tccgggtcga gcggatcgcg
     7381 ggaaaccacg cacgctttat cgagatagaa accgccatca ccgtcgttta aacgaaacag
     7441 cggcagaata ttgaacaggt tgatctcgtc gccatcgacg gtgttctgcg cccaggctgg
     7501 attggcgcgg cgctccgggg caatggggaa gttatcccag cggcggataa actcatcaat
     7561 ctgtttttta accggggtgt ttggcggcag gccgagggaa atcgcgtggt tctgccagga
     7621 accgatggtg ttcattgcca cgcgggcatc gataaagccg cgaatattat caaaccaaag
     7681 cgcgggcgcg ccgtcgccga tacgcccggt ggcgttggct gcggctgcca gatccggttc
     7741 cgcgttcacc tcttcgctga ttttcagtaa ctggccgtgg tcatcaagcg cctgtaaaaa
     7801 gctgcgtaaa tcatcaaatg ccattattca ttctcctgag aaaaattccg ggcctgcggc
     7861 aatccttgcc agcgcctggc gtgaggatgt tcgaggccaa attgatccag cacgcgggct
     7921 accacgtggt ggacaatgtc atctaccgtt tcgggatggt tataaaaggc aggcatcggc
     7981 ggcaccatcg ccacgcccat gcgcgaaagt gcgagcatat tttcgagatg gattgtgcta
     8041 agcggcattt cacgcggcac cagcaccagt ttgcggcctt ctttgagcac gacgtccgcc
     8101 gcgcgcccca ccaggccatc ggcgtaacca gcgcggatac cggcgagcgt tttcatactg
     8161 catggaataa cgatcatgcc gtcggtacga aaggagccgg aagagatagt cgccgcctga
     8221 tccgccgggt tatggctgaa gtctgcgagg gcggcaacat cgcgagcgct gtaaggcgtt
     8281 tccagttcaa tcgtggtttt cgcccacttc gacatcacta gatgagtctc gacattcggc
     8341 atctcccgca gcgcttgcag taatgccaca ccaagaggcg caccggtagc ccctgtcatc
     8401 ccgacgatca gtttcattgc ttactctctt aattgttcgt atacgaacgt tattattaac
     8461 atactccttc atttccacag cggcaaggat tgaaaagtaa caacatcttc ttcgtcactg
     8521 aaaaaccgct atgatagcga cagagaatta gcccggagtt tacatggcgt tacgaaataa
     8581 agcgttccat cagttacgac agctttttca gcagcataca gctcgctggc agcacgagtt
     8641 acctgacctg accaaaccac agtacgcggt gatgcgcgcc attgctgata agcctggtat
     8701 cgaacaggtg gcactgatag aagcagcagt cagcaccaaa gcaacgctgg cagaaatgtt
     8761 ggcaagaatg gaaaatcgtg gcttagtcag acgtgaacat gacgccgctg acaagcggcg
     8821 acgctttgtc tggctgactg ccgaaggaga aaaggtactt gctgcggcga tccccattgg
     8881 tgacagtgtg gatgaggaat ttttggggcg tttgagtgca gaagagcagg agctgtttat
     8941 gcagttggtg cgcaagatga tgagcaaata ggatgctgat tgccgggtag gacgctgacg
     9001 agtcttatcc aggcgacaaa acacaccgac ttcccaaaca aaagaaaaag gccagcctcg
     9061 cttgagactg gcctttctga cagatgctta cttattcgcg gaacagcgct tcgatattca
     9121 gcccctgcgt ttgcaggatt tcgcgcaaac ggcgcaggcc ttcaacctga atctggcgaa
     9181 cacgttcacg ggtgaggcca atttcacgac ctacatcttc cagtgttgcc gcttcgtacc
     9241 ccagcaaacc gaatcgacgt gccagtactt cacgctgttt ggcgttcagc tcgaacagcc
     9301 atttgacgat gctctgcttc atatcgtcat cttgcgtggt atcttccgga ccgttctctt
     9361 tttcatcggc caggatgtcc agcaacgctt tttcggaatc accacccagc ggggtgtcta
     9421 ccgaggtaat gcgctcgtta agacgaagca tacggctgac gtcatcaact ggcttatcca
     9481 gttgctctgc gatctcttcc gcacttggtt cgtggtccag tttatgggac aactcgcgtg
     9541 cggttcgcag gtaaacgttc agctccttta cgatgtgaat aggcaaacga atagtacggg
     9601 tttggttcat aatcgcccgt tcgatcgtct ggcgaatcca ccaggttgcg tatgttgaga
     9661 agcggaaacc acgttccggg tcaaacttct ctaccgcgcg gatcagcccc aggttgccct
     9721 cttcgatcag gtccagcaac gccagaccac gattgccata acggcgggca atttttacca
     9781 ccagacgcaa gttactctcg atcatccggc ggcgggaggc aacatctcca cgcagtgcgc
     9841 gacgcgcaaa ataaacttct tcttcggccg ttaacagtgg tgaataacca atctcaccaa
     9901 ggtaaagctg agtcgcgtcc agcacacgct gtgtggctcc ctgcgataac agttcctctt
     9961 cggccaaatc gttatcactg ggttcctctt ctactaaggc cttttcgtca aaaacctcaa
    10021 ctccgttctc atcaaattcc gcatcttcat ttaaatcatg aactttcagc gtattctgac
    10081 tcataaggtg gctcctaccc gtgatccctt gacggaacat tcaagcaaaa gcctggttcc
    10141 gccgatttat cgctgcggca aataacgcag cgggtttacg gatttcccct tgtaacgaat
    10201 ttcaaaatgc aagcgtgttg aactggttcc ggtgctaccc atggttgcta ttttttgccc
    10261 cgccttcact tcttgttgtt cccggaccag cattgtgtcg ttatgggcgt aggcactcag
    10321 gtaatcatca ttatgtttga tgataatcag attaccgtag ccgcgcagcg cgttaccggc
    10381 ataaacaacg cggccatctg cggtcgcgat aattgcctgt cctttgctgc ctgcgatatc
    10441 aatccccttg ttgcccccct cagaagcgcc aaaggtttcg atcactttgc cctcagtcgg
    10501 ccagcgccag gtggagatag gcgtactggt tgatgtactg ctgacagtcg gctcggttgt
    10561 gcttgctgtt ggtaccgtta caggcgctgt gaccgtggtc gcagttggct tgttgttcgg
    10621 caacattttg ttagcactct gttcacccga agactcagaa tacgtaattg tcggttgcga
    10681 cgcaacagca acggtggaat tttgtgcagg cttgatcaca actccttgct ctgctgcgtc
    10741 ggcctgggta atggcatttc cgccagtgat tggcgtaccg gaagcattac ccacctgtaa
    10801 ggtctgaccg acgttcagcg cgtatggtgc ctgaatattg ttgcgctgag caaggtcacg
    10861 gaaatcgttg ccagtaatcc aggcgatata gaaaagtgtg tcgccttttt tcacggtata
    10921 ggtactgccg ctataactgc ctttcggaat gttcccatac tgacggttgt agacgatgcg
    10981 tccgttttcc atctgtaccg gctgctgagc tactggctgt actggctgga tttgcggttg
    11041 ttgagtagcc tgaatttgtg gctgctgtac cggctgaatt tgcggttgct gcgctgtaga
    11101 cgtcgtcccc attttcggcg gcggcgtaat caacatacca gaattggtat ttgcaggcgc
    11161 attgccatta acggagctga ccggtgccgg tggatttgaa gtgtcagaac agcctgccag
    11221 ccatagcgaa accagtgaca aagccgcaat gcggcgaacg gtgaattttg ggcttcccgc
    11281 gctcatttat cccccaggaa aaattggtta ataaccagtg acataattac cgtgcaaggc
    11341 accatactga acactggaaa agatgttcac gatacgctga cctgcggcaa aataaccagg
    11401 aaaaatccag gtatttcctc acgttttaag ccagctcacc cttcactaaa gggacaaagc
    11461 gcacggcctc cacggtatcg ataataaatt cgcctccccg acgacgcacc cgtttcaaat
    11521 actggtgctc ctcccctacg ggtaagacga gaattccgcc ttcgtccagc tgcgtcatta
    11581 gcgcagttgg aatttccggc ggtgccgccg taacaatgat agcgtcaaac ggcgcacgtg
    11641 cctgccaacc ttgccatcca tcgccatgac gggttgaaac attatgtaaa tcaagatttt
    11701 tcaggcggcg acgcgcctgc cactgcaagc ctttaatccg ttcaaccgag caaacatgct
    11761 ggacaagatg cgccaggatt gccgtttgat atcccgaacc ggtgccaatt tccagcaccc
    11821 gcgactgcgg cgtcagttcg agtaattcgg tcattcgcgc caccatatac ggctgcgaaa
    11881 ttgtctgccc ctgacctatc ggcaaggcga tattgtccca ggctttttgt tcaaacgctt
    11941 catcaacgaa tttttcacgc ggcacggcgg caagtgcatt cagcaccagc tcatcctgaa
    12001 taccttgcgc acgtaattga tccagaagtg cttgtacgcg tctgcttacc attgcgtgcc
    12061 aactcccacg ctgtttaacc agtctgaaac cacatcttgc gcgctatgcg cagttaaatc
    12121 cacatgcagc ggcgtgatgg aaacatagcc ctcatctacc gcagcaaaat cggtccccgg
    12181 accggcatca catttaccgc ccggcgggcc aatccagtac agcgtattgc cgcgcggatc
    12241 ttgctgcggg atcacctgat ctgccggatg tcgtgtaccg cagcgcgtca cgcgaatacc
    12301 tttgatttga tccaatggta aatccggaac gttaatatta agaatacgtc cggtgcgcag
    12361 cggctcttta cacagtgcgc gcaaaattga acaggttacc gccgcggcag tgtcgtaatg
    12421 tttatgcccg tcaagcgaga cggccagcgc cggaaaacct aaatgacggc cttccatcgc
    12481 ggcggctacc gtaccggaat aaataacatc atcccccaga ttcggcccgg cgttaattcc
    12541 ggacacaaca atgtccgggc gcggacgcat cagagcattc acgccaagat agacgcaatc
    12601 ggtcggggtt cccatttgca cagcaatatc accattttca aaggtaaacg tgcgcaggga
    12661 ggattccagt gtcagagaat ttgaagcgcc gctgcggtta cgatcggggg cgaccacctg
    12721 aacgtcagca aactcacgca aggctttcgc cagcgtttgt ataccgggtg catgtacccc
    12781 gtcatcatta ctcagcaata tgcgcataat cacctgttgt gttgataagt tccctgacaa
    12841 cgctggttgc aaaacttccc gccggaagac agaaacggat ttctacggtg acgtcatccc
    12901 accagttcca gcttaatttt tgcggataca gcagcatcgc tctgcgcgcg gcttcaactt
    12961 tttcacgcac cagtaaagtt tgtaattcag tttcttcggc gacagctgct tgttcgaatg
    13021 ccagcgcttc acgctgagtt ccccattcgc cactgcccgg caatgcggcg gttatcatca
    13081 gctctttatc gttgacgcga cgctgtaatt ccgccagttc ttcggtggtt gcaacaaacc
    13141 agctaccacg tccggctaat tgtagcgcat cgccgtcaac aacttgatta acgtctgctt
    13201 ttttgaggcg ctcagcaaca atctgattaa acaacgcact gcgggctgcc gacaaccaaa
    13261 aactccgttt attgcgatcg cgcaccggag tattggtttg cgcccaacgc agcgcgccct
    13321 gcaagttgct accgccaatc ccaaaacgtt gggcaccgaa gtagttcggt acacctttta
    13381 cgcaaatatc gatcagacgt tgttcaacgt catcgcgatt gctcacttcg cgtagtacca
    13441 gggtaaaggc gttacctttc agcgccccta aacgcagctt gcgcttgtgc cgcgcatact
    13501 ccagcacctg gcagccttcc agttgaaagg cgctcagatc gggcatctct ttgcccggca
    13561 cgcgagcgca taaccactgt tccgtaacag catgtttatc tttttgccca gcgaagctga
    13621 cttcacgggc atgaattttc aggaatttcg ccagtgcatc cgccacaaaa cgggtattgc
    13681 agccgttttt gaggattcta accagaatat gctcaccttc accatcaggc tcaaagccca
    13741 aatcttccac caccacaaag tcttccggat tggctttcag cagcccggtg ccttgcggtt
    13801 taccgtggag gtaagtgaga ttatcaaact caatcatttt gttgccttaa tgagtagcgc
    13861 caccgcttca caggcaatcc cttccccacg tccggtaaat cccagttttt ccgtagtagt
    13921 ggctttcacg ttaacatcat ccatatggca gccgagatct tcggcaataa acacgcgcat
    13981 ttgtggaatg tgcggcaaca tcttcggtgc ctgagcgatg atagtgacat cgacgttgcc
    14041 gagggcataa cctttcgcct gaatacgacg ccaggcttcg cgcaacagct cgcggctgtc
    14101 ggcaccttta aatgccggat cggtatccgg gaacagcttg cctatatctc ccagcgccgc
    14161 cgcgccaagc aatgcatcgg tcaacgcatg gagcgccacg tcgccatcag aatgcgccag
    14221 cagccctttt tcgtaaggaa tgcgtacgcc accaatgata attgggcctt caccgccaaa
    14281 ggcgtgtacg tcaaaaccgt gtccaattcg cattatgtat tctcctgatg gatggttcgg
    14341 gtgaggtaaa attcggccag cgccaaatcc tccgggcgcg ttaccttaat gttatccgcg
    14401 cggccttcaa caagttgcgg atgaaaaccg caatattcca gcgccgaggc ctcatcggta
    14461 atggtagcgc cttcatttag agcgcgcgtc aggcagtcat gtaacagctc acgagggaaa
    14521 aattgcggcg tcagcgcgtg ccataagccg ttgcgatcaa cggtatgagc aatggcattt
    14581 ttgcccggtt cggcacgttt catcgtatcg cgcaccggcg cagcgaggat cccccccgtg
    14641 cggctggttt cgctcaacgc caacaatcgc gcgaggtcat cctgatgcag acaaggacga
    14701 gcggcgtcat gcaccaatac ccactgcgcg tcgccagcgg ctttcagacc tgccagcacg
    14761 gaatcggcac gctcttcacc accatcaaca accgtgattc gtggatgatt cgccagagga
    14821 agttgtgcaa agcggctatc gccaggactt atggcaatga cgacacgttt caccctggga
    14881 tgcgccagca gcgcatacac cgagtgttca agaatggttt gattaccgat tgagagatat
    14941 tgcttaggac attccgtttg cattcggcgg ccaaatccgg ccgccggaac cacggcgcaa
    15001 acatccaaat gagtggttgc catgttaatt cccgggctga tttatcgatt attttgcccc
    15061 gcagactgtg cgcgctttga cgcgtcaggc accagacgat aaaacgtttc gcccggcctg
    15121 gtcatgctga gttcattacg cgcacgctct tcgagcgcct cctggccgcc attgagatcg
    15181 tcaatttcgg caaaaagctg atcgtttcgc gctttaagtt tcgcgtttgt agcttgctgt
    15241 gccgccacat cattattgac gcgagtatag tcatgtatac cgttcttacc gaaccacagc
    15301 gaatactgta gccagaccag aatagccagc aacagcagcg ttagtttacc catcctgccc
    15361 cctgaaaaac ggcatcttca tcccatgcat ccgaagacga ctctacatcc tctgttgggg
    15421 ataccgcgac aacgcgggca aatgtaccac atttgtccat tgttacgtat acccagggcg
    15481 tgcagaacat aatctcatta ttagttacgg attgaattat gaacagaata gagaggaaag
    15541 aacaattaac ccagtagcca cataaacagc gcaccaaaca taatgccaac cgtcgccaga
    15601 gtgaaaacaa tactgtagcg tagctttccg tccatcaatg aatgcagtgc aatccccacc
    15661 actacagcga cgggcatcag cgccagaaag aaaggccagg tgtagataaa aaagagcagc
    15721 gtgttagagc cataaatcaa catcggaatt gccaacgcgc acagccacga taaaaaacca
    15781 accacagcgc ctggcagcga ccatgtggtg tcttcaacct cagtaaggct gtcgttattt
    15841 gttaatgtaa tgttgtggct gttacgcata tctgatcctg ttactttgac gaaccgggca
    15901 tggaaacccg gcggtgtctc aggatctgat aatatcgttc tgtctcaaca gatctaataa
    15961 ttgctgtacc aaatttgtta ctaattgttc accattgaga tgaatttctg ccgattcagg
    16021 cgcttcgtaa acggaatcta ttcccgtaaa gttgcgcagt tcaccggcac gcgctttctt
    16081 atataaacct tttggatcgc gggcttcgca aatcgccagc ggcgtatcga caaacacttc
    16141 gataaagcgc ccttctccta cgcgttcgcg aaccatctgg cgttcggcgc ggtgtggcga
    16201 gataaatgcg gtcagcacca ccagtccggc ttcaaccatc aaattcgcca cttcaccaac
    16261 ccgacggata ttctctttac ggtcggcatc gctaaaaccg agatcgctgc ataatccgtg
    16321 gcgaacgttg tcgccatcca gcagatacgt actgacgcca agtttatgta acgcctcctc
    16381 cagcgccccc gcgacggttg atttaccgga accagagagg ccggtaaacc acagcacaac
    16441 accacgatga ccgtggtgct gttcgcgttg ttgcggagtt actgggtggc tatgccagac
    16501 gacgttttcg tcatgcagcg ccattattta tctcccagta aatcgcgcgc accccaatgc
    16561 gggaaatggc ggcgaaccag ggcattcaat tccagttcga atgcactgaa ttcagatggc
    16621 gcagcagttg cctggctaac tggttcgtgc accataccgg cacctacggt cacattactc
    16681 aggcgatcga taaaaatcag cccaccggta accgggtttt gctgataacg atctaacacc
    16741 agtggctcgt caaaggtgag atctaccagg ccgatgccgt tcagcggcag gttttcaact
    16801 tcgcgttggg taaggttatt gatatcaacc tgataacgaa tgccatcaac acgagcacgc
    16861 gtcttcttac cggcaatttt gatgtcataa ctctggcccg gggaaagcgg ctgttccgcc
    16921 atccatacca catccaccga cgcgctctgc acagccggta acgcttcgtc tgccgccagc
    16981 agcagatcgc cacggctgat gtcgatctca tccgtcagca ccagggtgat cgcttctccg
    17041 gcaaaggctt cttcgcgatc gccatcaaaa gtcacgatcc gcgcgacgtt tgattccaca
    17101 ccagagggca gcactttgac acgttgcccg acttccacgc gaccggatgc cagcgttccg
    17161 gcgtagccac gaaaatcgag attcggacgg ttaacgtact gcaccgggaa gcgcattggc
    17221 tgagcatcca ccactcgctg gatctccacg gtttccagca cttctagcag tgtcggaccg
    17281 ctgtaccacg gcatactttc actttgcgaa gccacgttgt cgccttccag tgcggagagc
    17341 ggcacaaagc ggatatccag attacccggc agctgcccgg caaaagtcag ataatcttca
    17401 cgaatacggg tgaacgtctc ttcactgtaa tccaccagat ccattttgtt gatcgccacg
    17461 accagatgtt tgatccccaa cagtgtggag ataaaactat gacgacgggt ttgatcgagc
    17521 acgcctttac gggcatcgat cagtaagatc gccagttcac atgtcgatgc gccagtcgcc
    17581 atattgcggg tgtactgctc gtgccctggg gtgtcggcga taataaattt acgcttctcg
    17641 gtagagaaat agcggtaggc cacatcaata gtgatgccct gttcgcgctc agcttgcagg
    17701 ccgtccacca gcagtgccag atccagcttt tcgccctggg tgccgtgacg cttactgtcg
    17761 ttatgcagcg atgagagctg atcttcgtag atttggcggg tatcgtgcag caggcgacca
    17821 atcagggtac ttttgccgtc atcgacgcta ccacaggtca gaaaacgcag caggctttta
    17881 tgttgttgcg caatcatcca tgcttcgacg ccgccttcat tggcgatttg ttgtgcaagt
    17941 gcggtgttca tcttaaaaat acccctgacg ttttttcagc tccatagacc ccgcctggtc
    18001 gcggtcaatt acgcggccct gacgttcact ggtggtggaa accagcatct cttcgatgat
    18061 ctccggcagt gtttgcgcat ttgactccac cgcgccggtc agcggccaac agcccagtgt
    18121 gcggaaacgc accatccgtt ttttaatcac ttcgcccggt tgcaggtcga tacggttgtc
    18181 atcaatcatc atcaacatac cgtcgcgttc cagaaccgga cgttccgcag caagatacag
    18241 cggaacaatg tcgatatttt ccagccagat gtattgccag atatcctgct cggtccagtt
    18301 agagagcggg aagacgcgga tgctttcgcc tttgttaatt tgcccgttgt agttgtgcca
    18361 cagctccggg cgctgatttt tcggatccca gcgatggaag cggtcacgga aagagtagat
    18421 tcgctcttta gcgcgggatt tctcttcgtc acggcgcgca ccaccgaagg cggcatcaaa
    18481 accgtatttg ttcagcgcct gtttcaggcc ttcagttttc ataatatcgg tatgtttcgc
    18541 gctgccgtgt acgaatggat taatccccat cgccacgcct tccgggtttt tatgcaccag
    18601 cagctcgcag ccgtaggctt tcgccgtacg atcgcggaac tcatacatct cgcggaattt
    18661 ccagccggta tcgacatgca gcaacgggaa aggcagcgta cctggataaa acgccttgcg
    18721 cgccagatgc agcatgacgc tggaatcttt accgatagag tagagcatca ccggatttga
    18781 gaattctgca gccacctcgc gaataatgtg gatgctttcc gcctccagtt gccgcaggtg
    18841 ggtaagtcgt ttttgatcca taaccgttcc tttgcaatac cgctattttc ttgccatcag
    18901 atgtttcgac tatagggagc gtaagagaac gaatgaaatt accaattaga atgagtagtt
    18961 ccttaacgga ataacgattt ggcaaagcta atatcaaaaa gtgcttaagg caccggattt
    19021 cgggcgttta ggaagatttg aaattgtttt agcgcagcgg cagtttcata ctatgacggt
    19081 aaaaaaattt gcatggtatt taaggactca ctatgttttc cgcattgcgc caccgtaccg
    19141 ctgccctggc gctcggcgta tgctttatcc tccccgtaca cgcctcgtca cctaaacctg
    19201 gcgattttgc taatacccag gcacgacata ttgctacttt ctttccggga cgcatgaccg
    19261 gaactcctgc agaaatgtta tctgccgatt atattcgcca acagtttcag caaatgggtt
    19321 atcgcagtga tattcggaca tttaatagtc ggtatattta taccgcccgc gataatcgca
    19381 agagctggca taacgtgacg ggaagtacgg tgattgccgc tcatgaaggc aaagctccgc
    19441 agcagatcat cattatggcg catctggata cttacgctcc gctgagcgat gctgacgccg
    19501 atgccaatct cggcgggctg acgttacaag gaatagatga taacgccgca ggtttaggtg
    19561 tcatgctgga actggcagaa cgcctgaaaa atacgcctac cgagtatggt attcgatttg
    19621 tggcgaccag tggtgaagag gaagggaaat taggtgctga gaatttactc aagcggatga
    19681 gtgacaccga aaagaaaaat acgctgctgg tgattaatct cgataactta attgttggcg
    19741 ataaattgta tttcaatagt ggtgtaaaaa cccctgaagc agtaaggaaa ttaacgcgcg
    19801 acagggcgct ggcaattgcg cgtagtcatg gaattgccgc aacgaccaat ccgggtttga
    19861 ataaaaatta tccgaaaggc actgggtgtt gtaatgacgc agaaatattc gacaaagccg
    19921 gtattgctgt actttcggtg gaagcgacta actggaatct tgggaataag gatggttatc
    19981 agcaacgcgc aaaaacagcc gcattccctg cgggaaatag ctggcatgac gtaagactgg
    20041 ataatcaaca acatattgat aaggcgcttc ctggaagaat agaacgtcgc tgccgtgatg
    20101 ttatgcggat aatgctacct ctggtgaagg agttggcgaa ggcgtcttga tgggtttgaa
    20161 aatgggagtt gggagttcta ccgcaggggc ggggaattct aagtgatatc catcatcgca
    20221 tccagtgcgc ccggtttatc cccgctgacg cggggaacac acatgaatgt cggttcagac
    20281 cgtgttttta cccggtttat ccccgctggc gcggggaaca cgaggctgaa cagggagggg
    20341 gctaccggca cagcggttta tccccgctgg cgcggggaac acttgccaac cgccaaagac
    20401 tggaaaacca atctcggttt atccccgctg gcgcggggaa cacgaactgg cgttgagtga
    20461 taaatttgac ggaatcggtt tatccccgct ggcgcgggga actcttcagt ttcacactcg
    20521 gtaatgccgt tatttacggt ttatccccgc tggcgcgggg aactcctcgg ggccagcctc
    20581 ccccattaac ccggcaccgg tttatccccg ctggcgcggg gaactctcta aaagtataca
    20641 tttgttctta aagcat
//
LOCUS       DAZUCC010000076        19963 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZUCC010000076 DAZUCC010000000
VERSION     DAZUCC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.076"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHY09_002397"
     CDS             complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHY09_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFS3885359.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1564..2763)
                     /gene="hemX"
                     /locus_tag="ACHY09_002398"
     CDS             complement(1564..2763)
                     /gene="hemX"
                     /locus_tag="ACHY09_002398"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFS3885360.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQADAP
                     AAAPQGE"
     gene            complement(2785..3525)
                     /gene="hemD"
                     /locus_tag="ACHY09_002399"
     CDS             complement(2785..3525)
                     /gene="hemD"
                     /locus_tag="ACHY09_002399"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFS3885361.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CVIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3522..4463)
                     /gene="hemC"
                     /locus_tag="ACHY09_002400"
     CDS             complement(3522..4463)
                     /gene="hemC"
                     /locus_tag="ACHY09_002400"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFS3885362.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYESLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4850..7396
                     /gene="cyaA"
                     /locus_tag="ACHY09_002401"
     CDS             4850..7396
                     /gene="cyaA"
                     /locus_tag="ACHY09_002401"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFS3885363.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     CNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7436..7756)
                     /gene="cyaY"
                     /locus_tag="ACHY09_002402"
     CDS             complement(7436..7756)
                     /gene="cyaY"
                     /locus_tag="ACHY09_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFS3885364.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7804..9861)
                     /locus_tag="ACHY09_002403"
     CDS             complement(7804..9861)
                     /locus_tag="ACHY09_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885365.1"
                     /translation="MINTSRQDGFMHFRNVVCLLSCSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGTR
                     TDDNGGENSLDIKTLHQVKFRSPWKPDFLGLILRDANFLYVDGHRLADPESFRVLAQK
                     SWDQRGKFSTTFNPCVAVPFGPWDTLARTRTKIIINGEQLKADPETFTVVRWMPGSLL
                     TWRDKSGLHRKVLNQGNLDKDRANSCAAFTLQEHSVSWRKGPECQQEVLPGLDPEQFH
                     PLSNTVAQYQDKLYTIKKTGFGENRLNIVTLDNPGLVIDKRFNAGRTHGYLLTKLPAD
                     GEEEDGLQVFDSSGPLILMDYRVPDEHEAHLSDSPHYKKWYAHDDRYVYAFDGSQLWR
                     YPTPNTKAVRVKWKTKDSGYGYWANYRSGELDGRLLEDGAFIPTGIPYNSSVNIAQYS
                     DGEGSFLVGETDVSWRKLRSTDQQWSQWEKLPDIDPKAFHPITDRVAQYKNHLYIAKL
                     SPFGEDTLEIITLDSSAPFLKQRINAGKGHGYFMRSSRLRDDIQIFAIDGPLKTTERF
                     AYDNRYVYTWIGSQLYRTASPCPARTRNLDQNMFSSNDDIIILTTEEECHKTPDIEQT
                     LKL"
     gene            complement(10203..10757)
                     /locus_tag="ACHY09_002404"
     CDS             complement(10203..10757)
                     /locus_tag="ACHY09_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFS3885366.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11099..11302
                     /gene="yifL"
                     /locus_tag="ACHY09_002405"
     CDS             11099..11302
                     /gene="yifL"
                     /locus_tag="ACHY09_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS3885367.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVEM
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11339..12163
                     /gene="dapF"
                     /locus_tag="ACHY09_002406"
     CDS             11339..12163
                     /gene="dapF"
                     /locus_tag="ACHY09_002406"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS3885368.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12160..12867
                     /gene="yigA"
                     /locus_tag="ACHY09_002407"
     CDS             12160..12867
                     /gene="yigA"
                     /locus_tag="ACHY09_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFS3885369.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDCWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12864..13760
                     /gene="xerC"
                     /locus_tag="ACHY09_002408"
     CDS             12864..13760
                     /gene="xerC"
                     /locus_tag="ACHY09_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS3885370.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13760..14476
                     /gene="yigB"
                     /locus_tag="ACHY09_002409"
     CDS             13760..14476
                     /gene="yigB"
                     /locus_tag="ACHY09_002409"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFS3885371.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14560..16722
                     /gene="uvrD"
                     /locus_tag="ACHY09_002410"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14560..16722
                     /gene="uvrD"
                     /locus_tag="ACHY09_002410"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFS3885372.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16767..17669)
                     /locus_tag="ACHY09_002411"
     CDS             complement(16767..17669)
                     /locus_tag="ACHY09_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFS3885373.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LKQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17752..18516)
                     /gene="yigE"
                     /locus_tag="ACHY09_002412"
     CDS             complement(17752..18516)
                     /gene="yigE"
                     /locus_tag="ACHY09_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFS3885374.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVG
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18678..18734
                     /gene="ysgD"
                     /locus_tag="ACHY09_002413"
     CDS             18678..18734
                     /gene="ysgD"
                     /locus_tag="ACHY09_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFS3885375.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18886..19836
                     /gene="corA"
                     /locus_tag="ACHY09_002414"
     CDS             18886..19836
                     /gene="corA"
                     /locus_tag="ACHY09_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFS3885376.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19872..>19963)
                     /locus_tag="ACHY09_002415"
     CDS             complement(19872..>19963)
                     /locus_tag="ACHY09_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885377.1"
                     /translation="DNNEYQLTQENKIERVFNPHDFEKDNFQQ"
BASE COUNT         4634 a         5198 c         5212 g         4919 t
ORIGIN      
        1 aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa ttatctcata
       61 acaagtgtta agggatgtta tttcccggtt ctctgtggca taataaacga gtagatgctc
      121 attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc agagccgttt
      181 acggtgctta tcgtccactg acagatgtcg cttctgcctc atcagacacc atggacacaa
      241 cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg ctccgtaata
      301 agagcaggcg tttttttatg tatcagaaag gtcccggagg agcttgcctc cgggtgagaa
      361 agagctactg tggcgggttg ttctgcaacg ttaacatcaa accgtcgcga cgcatcgctg
      421 cggcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg taatcgtagg
      481 cgtccggacg ttgtttcagc gctgcgcgga aggcgagcga tgcttcctgc cattcgccgt
      541 gcttcatcag cgactgaccc agtgtgctcc acaacagcgg gcgatcgccc acgtttttga
      601 tctgttggcg cagcactttt tcaagctgtt ccgggttatt ggttttcaga cgcgggatcg
      661 gcagcagcag gcgatcgtca tactggcgtt tcaggccgtc gatgataatt tgctgggcag
      721 tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgtaac gccacctgat
      781 gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcacta ccgttatcgg
      841 cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca cgatgttctt
      901 catcaccaac atgggctttc gccattgatg ggatgatatc cagcagcgaa ctccatgcac
      961 cagtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt ggcgtaactt
     1021 ccagcagctt atcaacgccg tgacgtgctg catggttttc attacgggcc agttgcagac
     1081 gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct gcgcgttcca
     1141 gatgttggtt ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggct gccagtagat
     1201 agttcaccac cggttgttca gcgtgatcgg catttttcgc catcagcttt tcaacttgct
     1261 gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgt gcacggcgac
     1321 gcttacgtcc gacaaaccac ccacgggtgt gcgcgcctgt gcggaagatt cgccgcagta
     1381 gccactcaat ggcaaacagc actaccatcg ccagaatcaa aataatcgcg aggcccgtga
     1441 cgctagtttc aatattgtag ttgtcggtct ggatcagcac gtaaccctga tggccggcaa
     1501 tcatcgggcc aaccacgatc cccgcaatca gcaacacaaa gagcaataac acttttagca
     1561 tggttattct ccttgcggcg cggctgccgg agcatcagct tgcggtgcag gtgcaggtgc
     1621 aggtgcaggt gcaggtttag cttccgttgt ccccgctgcc ggttgtgcca gcaggttacg
     1681 cacgcgagtt tgcatcagtt tttccagcat cgcctggctt tgcagggttt ccggaagatc
     1741 catcgagata ttttgctggc ttaactggtc cacctcgtcg aggaacgctt tggtggtggc
     1801 atcatcagta tcgtagtaag cacgtaccca ggtggagacg ttctccagcg cctggcgata
     1861 agtctcttcc tggtgacgcg gtacagcttg tgctgcgacc agcaggcgag agcgaatatt
     1921 ttcgcgcaga tagatatcct gatttggcgc taacagcggt acggcggtgt catcacgacg
     1981 gcgaatcgta atgaagttgt ccataaagtt ctgccagctt ttttgcagat tgatacgcca
     2041 ttcgctgatg gaactggaaa gctcttcacc gtcggaatcc atcggcgaac catcgctgtc
     2101 attatcagcc agacgcagat tatctacctg atttgaaagc tgattaagct taaggatgat
     2161 accgtcataa tccacctgcg ataccgcaga aaggctggcg atatcatcgg taattgcccg
     2221 acgaacggta atcagactcg ggtcattcat atccgccagg ctggcatctg cacttttcag
     2281 caacgctgca gcggtcgtga cgtcttgatc gctccacagc ttccgtcctg cgagtttcac
     2341 caggaaatca gcctgagcca gcagccaggt tttagcatcg ctgccggaaa tagtcgcgac
     2401 cttttgttgg acttcatcca gctgttttgc cagcgtttct tgctgacgat tcgcctgctc
     2461 aagctgtaca gcttgttgct tgataatgcc ttccagctca gctttttggc tctcctgggc
     2521 tttttgcagt gccgtaagtt ggttagccag ggcatcgctg gtggcagtct gattgacggc
     2581 ctgttgttta ccccagccat acaaaccgac gcccgccgcc agagcaatag cgatagccac
     2641 cgcgctgaga atcaatgcgg tattgttctt actctttttt tctgttgcga caggttgtga
     2701 cgtggtgtcc acggcctccc tggtctcttc aaccacggcg gaggtttttt cttgttccgt
     2761 cattatggct tcctgttatg agagttattg taatgcccgt aaaagcgcat cgttgtcagc
     2821 gttatcggcg accttaatgt cttgccagcc cagttcccgg gcgagtttcg ccaaacgctc
     2881 actgacgacc aatagtcgac agcgtagtaa ccagtgctca cgataccatt gtgggatcag
     2941 cgaccagagt tgctgcaaca tttcaccgct ggtaacaacg accgtcgtca cctcgcggga
     3001 ttgccagcgc atcgcttctt ctgcaccatc gtaatggatt acgcatcgtt gataacattc
     3061 acaaaaagtg acctcagcac cgcgcgccgt cagggtatcc ccaattagct cacgaccgcc
     3121 attgccacgt aatatcagcg cacgtttgcc cgcaatattt tgtaattcag gtaattgtag
     3181 caagacttcg ctgatttccc gatcctgcgg gtagagaatc ttctgtccgc ttacggtatg
     3241 tagtgccagt gcggtggtgc gtccaatggc gaaataagta ggtagtcggg gccatttacg
     3301 atcttgctga tgcagctgtg attgggcaaa agcaaccgcg tgttgcgaga gggcaaacaa
     3361 cagatcgctc tcccccagcg ccgccagttg atcagcaagt tgtggtaatt gtcgacccgg
     3421 agaaaactca atcagtggaa aatgccaggc cacctgcccc agtgtgcgca gacggctcac
     3481 taactcttct ccagcgggag acgggcgggt gacaaggata ctcatgccgg ggcgtctcca
     3541 ttatagactt cagcgaggat ctcgcgcgcg ccgttattca gtagctcttc tgccagcgaa
     3601 atccccattt gttcggcatc ttgcggcgca ccgcggcgtt caccgcgaat aatctgcgaa
     3661 ccgtccggcg cgccgaccag cgcacgcagc cagatttcac catcaataag ctcggcgtag
     3721 ctaccaattg gcacctgaca tccgccttcg agacgggtat tcatggcgcg ttctgcggta
     3781 acgcgcagtg cagtttcgtg gtgattcagc gcggcaagca gctcgcgagt gcgtgtatca
     3841 tcaaggcggc attcaatacc gaccgcacct tgtcctaccg ccggaagaga aatctcgggt
     3901 ggtaacgcgg cgcgaatacg tgactccaga cctaaacgtt ttagtccggc tacggcaaga
     3961 atgatggcat cgtattcgcc gttatccagt ttactcaggc gagtgccgac gttgccgcgc
     4021 agggagcgga taatcagatc cgggcggcgt tcagccagtt ggcactggcg acgtaaactg
     4081 gacgtcccga cgatactgcc tgccggtaac gcatccagac tttcatagtt attggacaca
     4141 aaggcatcgc gaggatcttc acgctcgcaa atggtgacca gccccagacc ttgcgggaat
     4201 tcaaccggca catctttcat tgaatgtacg gcgatatcgg cgcgattctc caggagcgcg
     4261 acttccagct ctttaacaaa taagcctttt ccgcctactt tcgccagcgg cgtatcaaga
     4321 atcacatcgc cgcgcgtcac catcggtacc agttcaacga ccaggcccgg atggctcgcc
     4381 atcaacttgt ctttgacata gtgtgcctgc cagagtgcaa gtgggctttg gcgtgtggca
     4441 attcttaaaa cattgtctaa catgcttgtt accgtcatta tcatccgtgg tccatcctaa
     4501 catccttgcc agagcgatgt cagtgttgtg gtgaaacgta gacgcctgcg caaaccgtaa
     4561 aatgaggtct ggcagtggat cctgacaggc gtttcacgcc gtcgtaataa ggaatttaca
     4621 gagaataaac ggtgctacac ttgtatgtag cgcatctttc tttacggtca atcagcaagg
     4681 tgttaaattg atcacgtttt agaccatttt ttcgtcgtga aactaaaaaa accaggcgcg
     4741 aaaagtggta acggttacct ttgacatacg aaatatcccg aatgccgcgt gttaccgttg
     4801 atgttggcgg aatcacagtc atgacgggta gcaaatcagg cgatacgtct tgtacctcta
     4861 tattgagact ctgaaacaga gactggatgc cataaatcaa ttgcgtgtgg atcgcgcgct
     4921 tgctgctatg gggcctgcat tccaacaggt ctacagtcta ctgccgacat tgttgcacta
     4981 tcaccatccg ctaatgccgg gttaccttga tggtaacgtt cccaaaggca tttgccttta
     5041 cacgcctgat gaaactcaac gccactacct gaacgagctt gaactgtatc gtggaatgtc
     5101 agtacaggat ccgccgaaag gtgagcttcc aattactggt gtatacacca tgggcagcac
     5161 ctcgtccgta gggcaaagtt gttcctctga cctggatatc tgggtctgtc atcaatcctg
     5221 gctcgatagc gaagagcgcc agttactaca acgtaaatgc agcctgctgg aaagctgggc
     5281 cgcctcgctg ggcgtggaag ttagcttctt cctgattgat gaaaatcgct tccgtcataa
     5341 tgaaagcggc agcctggggg gcgaagattg tggctccacc cagcatatac tgctgcttga
     5401 cgaattttac cgtaccgccg tgcgtctcgc tggtaagcgt attctgtgga atatggtgcc
     5461 gtgcgacgaa gaagagcatt acgacgacta tgtgatgacg ctttacgcgc agggcgtgct
     5521 gacgccaaat gaatggctgg atctcggtgg cttaagctcg ctttctgctg aagagtactt
     5581 tggtgccagc ctttggcagc tctacaagag tatcgattcc ccatacaaag cggtgctgaa
     5641 aacactgctg ctggaagcct attcctggga atacccgaac ccacgtctgc tggcgaaaga
     5701 tatcaaacag cgtttgcacg acggcgagat tgtgtcgttt ggcctcgatc cttactgcat
     5761 gatgctggag cgtgttactg aatacctgac ggcgattgaa gatttcactc gtctggattt
     5821 agtacgccgc tgcttctatt taaaagtgtg cgaaaaactc agccgtgaac gcgcctgcgt
     5881 aggctggcgt cgtgaagtgc taacccagtt agtgaaagag tgggaatggg acgatgctcg
     5941 tctggcaatg ctcgataacc gcgctaactg gaagattgat caggtacgtg aggcgcacaa
     6001 cgagttgctc gacgcgatga tgcagagcta ccgtaatctg attcgctttg cgcgccgcaa
     6061 taaccttagc gtctccgcca gcccacagga tattggcgta ctgacgcgta aactgtatgc
     6121 cgcgtttgaa gcgttaccgg gtaaagtgac gctggtaaac ccgcagattt cgcccgatct
     6181 ctcggaaccg aatctgacct ttatttatgt gccgccgggc cgggctaacc gttcaggttg
     6241 gtatctgtat aaccgcgcgc caaatattga gtcgatcatc agccatcagc cgctggaata
     6301 taaccgttac ctgaataaac tggtggcgtg ggcatggttt aacggcctgc tgacctcgcg
     6361 tacccgcttg tatattaaag gcaacggcat tgtcgatttg cctaagttgc aggagatggt
     6421 cgccgatgtg tcacaccatt tcccgctgcg cctgcctgca ccgacgccaa aagcgctcta
     6481 cagcccgtgt gaaattcgcc atctggcgat tatcgttaac ctggaatatg acccgacagc
     6541 tgcgttctgc aatcaggtgg tgcattttga tttccgtaag ctggacgtct tcagctttgg
     6601 cgagaatcaa aattgcctgg taggtagcgt ggacctgctg taccgcaact cgtggaacga
     6661 agtgcgtacg ctgcacttca acggcgagca gtcgatgatc gaagccctga aaaccattct
     6721 cggtaaaatg catcaggacg ccgcaccgcc agatagcgtg gaagtcttct gctacagcca
     6781 gcatctgcgc ggattaatcc gtactcgcgt gcagcaactg gtttctgagt gcattgaact
     6841 gcgtctttcc agcacccgcc aggaaaccgg gcgcttcaag gcgctgcgcg tttctggtca
     6901 aacctggggc ttattcttcg aacgcctgaa tgtatcggta cagaaactag aaaatgccat
     6961 cgagttttat ggcgctattt cgcataacaa actgcacggc ctgtcagtgc aggttgaaac
     7021 caatcacgtc aaattaccgg cggtggtgga cggctttgcc agcgaaggga tcatccagtt
     7081 cttcttcgaa gaaacgcaag acgagaatgg ctttaatatc tacattctcg acgaaagcaa
     7141 ccgggttgag gtgtatcacc actgcgaagg cagcaaagag gagctggtgc gtgacgtcag
     7201 tcgcttctac tcgtcatcgc acgaccgttt tacctacggc tcaagcttca tcaacttcaa
     7261 cctgccgcag ttctatcaga ttgtgaaggt tgatggtcgt gaacaggtga ttccgttccg
     7321 caccaaatcg attggcaaca tgccgcctgc caatcaggat cacgacacgc cgctattaca
     7381 gcagtatttt tcgtgatgag cgtgccggaa agcgaggctt atccggcatg caatcttagc
     7441 ggaaactgac tgtttcaccc gcttgctgcg ttgccgcctg ttccagcaaa tcccagaagg
     7501 tttcgccgct acgatcgcaa atccactcat cgcctttcag atcaaaatgg tagccgccct
     7561 gtttggttgc cagccatacc tggtgcagcg gttcctggcg gttgataatg attttgctcc
     7621 cgttttcaaa ggtaatggtc agtacaccgc cgttgatttc gcagtcgata tcgctatcgc
     7681 catcccagtc gtccagacgc tcttcaatgg tcagccacag ttgatcagcc aggcgatgaa
     7741 attcactgtc gttcattgtt gtatcctgtt tttaagtgat ggcggcagta tagcggcatg
     7801 gggtcagagc ttcaaagttt gctcaatgtc tggtgttttg tggcactctt cctccgtcgt
     7861 caagatgatg atgtcatcat tggatgaaaa catgttctga tccagattac gtgtcctggc
     7921 cggacagggc gatgcggttc gataaagttg gctaccgatc caggtataaa catagcgatt
     7981 gtcataagcg aaacgttcgg tggttttcaa tggtccatca atagcaaaaa tttgaatatc
     8041 atcgcgtaaa cgggaagagc gcataaagta accatggcct ttcccggcgt taatccgttg
     8101 tttcaggaaa ggtgcagaag agtcgagcgt tattatttcc agggtgtctt caccaaaagg
     8161 agacaatttc gcaatgtaca gatgattttt gtactgcgcg acacgatcgg tgatgggatg
     8221 aaatgctttc gggtcaatat cggggagttt ctcccattga ctccattgct ggtctgtaga
     8281 gcgaagctta cgccagctaa catcggtttc gccgacgaga aaagagcctt cgccatcaga
     8341 atattgagca atgttaacgg atgagttata ggggattccc gttggaataa acgcaccatc
     8401 ctcaagaagt ctgccgtcaa gctcaccact acggtagttt gcccaatagc catatcctga
     8461 atctttggtt ttccatttca cccgcacggc tttggtattg ggagttgggt agcgccaaag
     8521 ctgtgagcca tcgaatgcgt aaacataacg atcgtcgtgt gcataccatt tcttgtaatg
     8581 ggggctatcg ctaaggtgag cctcatgctc gtcaggaaca cgataatcca tcaggataag
     8641 cggcccggaa gaatcgaata cctggaggcc atcttcttct tcaccatcag ctggtaattt
     8701 ggtcaataaa tagccgtgag ttcttcccgc gttaaagcgt ttatctatca ccaaaccagg
     8761 attatccagt gtgacgatat ttagcctatt ttcgccaaat cccgtttttt tgatggtgta
     8821 cagcttgtcc tgatactgag caacggtatt actaagagga tgaaattgtt ctggatcgag
     8881 gccgggtagg acttcttgct ggcattctgg ccctttgcgc catgaaacag agtgttcctg
     8941 caaagtgaac gccgcgcagc tatttgccct atctttgtcc agatttccct gattcagcac
     9001 cttccggtgc aggccgcttt tatctcgcca ggttaataac gaaccgggca tccagcgtac
     9061 gacagtaaat gtctctgggt cggctttaag ctgttcgcca ttgattatga ttttggtccg
     9121 tgtacgggct aacgtatccc aggggccaaa tggtacggca acgcaggggt taaacgtggt
     9181 ggaaaacttt cctcgctgat cccaggactt ctgcgccagc acacggaaac tctcggggtc
     9241 agcgaggcga tgtccatcga catataagaa attagcgtca cgtaatatca gccccagaaa
     9301 gtcaggtttc cagggggaac ggaatttcac ctgatgcagc gttttaatat ctagactatt
     9361 ttctccgcca ttgtcatcgg tgcgcgtgcc ttcaaaatag agactgtgtt tatctgcggc
     9421 aaaatcaccc catgccttga acgaggcgac atcgacggga ggtttgtctg gcgggttttg
     9481 cacaacttca cccgcgtaga ggatatagcg accatcgcta tgccaggagt aatgaggata
     9541 ccaggcctgt gcgccttcat agaggtagtc ggttgtcaga tcagtcagag gaggtacttt
     9601 atcccatttg tctagtgttg tcgggtctat taataaattg ggtaaacggc gcaaactttt
     9661 gtaattcacg tcaggaagag aaagatttgt ttctttataa gggcgcaggt ataccacgcc
     9721 ttttccctgt ttctggtaaa tattatgttc aggcgcggaa agccgacatc cccatgccga
     9781 cgataaaaac aacgaacaag acaacaaaca aaccacattg cgaaagtgca taaagccatc
     9841 ctggcgcgag gtgttgatca cgaaactgct agcagaacat aaatcctcac aagtaagcgt
     9901 aactctcgca gcaaatcgtc acttttcaga atccataaag gtcatcgcta ttttctgata
     9961 gttgctggaa taaacgtgat attttcattc acgatataaa cacaatcccg gaactgcccc
    10021 atagtacggc taatagcata aaaatattcc ggattgaccc ctggaaggga ggcaatgtgg
    10081 gattccgccc cccgttgcct tctttattgt ctttcacgag ttgtacccag gttccaccac
    10141 gggtatctaa cattcgccac attattacgc tttctgccga acgtgatttt tatagaaatc
    10201 tatcaattca ttttccctgt accataaagt ttatccttca catcctgccg aacgcgggtc
    10261 ttgtaccccc cgttgtgaag atcttcatac ggagaaaaag aatctttact ccaccccagc
    10321 aacactttat cgccaggcat aaaatgcacg tgcaggtaat tttctcccca ttctcttttt
    10381 gggagaggta acgtcatatt gcgtttctcc agacgcattc cagcattata ctgtgtacga
    10441 gtagtactta atgtccatat aacctctgcc gtatccccac tgacatcacc gcaacaggtg
    10501 attgaaccgc caccattcgc aaaagcattc gcgcccgcca cgccgttaac actgaatccc
    10561 agtatcggac ggtcaatttc actgtggatt tccagtatca ctccacctct tggtcctccc
    10621 catactgcct gtcctatatt ccacaacacg accagtactg gaacgacaat taacgtccgc
    10681 catacccagc gcccccagcg ggcataaccg cgcgtaagct tttggtcaaa tttttccagt
    10741 tgggtaaata gccccatcga ttttattccc tcttctcacc tggttggcaa agttgcacgt
    10801 cactggcgac taaggtcaga caacgaaatt tcgtggtacg ccactctctt aaaagtccca
    10861 tgcaggcgat aagaacaaga caacaaacaa accacattgc gatagtgcat aaagccatcc
    10921 tggcgcgagg tgccgatcac gaaactacca gcaaaacata aatcgccacg tgtaagcgtt
    10981 atactcgcag catttcctca cttttcagac ttcataaaga gtcgctaaac gcttgctttt
    11041 acgccttctc ctgcgatgat agaaagcaga aagcgatgaa ctttacaggc aatccataat
    11101 gaaaaacgtg tttaaggcac tcactgtatt acttactctc ttcagcctga cgggctgcgg
    11161 tctgaaaggt ccgctctatt tcccgcctgc agataaaaac gcaccgccgc cgaccaaacc
    11221 ggtagagatg caaacgcaat ccacggtgcc ggataaaaac gaccgcgcca ctggcgatgg
    11281 tccatcccag gtgaattact aaaagtcagt ttctgtaccc gcgtgattgg agtaaatgat
    11341 gcagttctcg aaaatgcatg gccttggcaa cgattttatg gtcgtcgacg cggtaacgca
    11401 gaatgtcttt ttttcaccgg agctgattcg tcgcctggcg gatcggcacc tgggggtagg
    11461 gtttgaccag ctgctggtgg ttgagccgcc gtatgatcct gaactggatt ttcactatcg
    11521 cattttcaat gctgatggca gtgaagtggc gcagtgcggc aacggtgcgc gctgctttgc
    11581 ccgttttgtg cgcctgaaag ggctgactaa taagcgcgat atccgcgtca gcaccgccaa
    11641 cgggcggatg gttctgaccg tcaccgatga tgatctggtc cgcgtaaata tgggcgaacc
    11701 caacttcgaa ccttccgccg tgccgtttcg cgctaacaaa gcggaaaaga cctatattat
    11761 gcgcgccgcc gagcagacaa tcttatgcgg cgtggtgtcg atgggaaatc cgcattgcgt
    11821 gattcaggtc gatgatgtcg ataccgcggc ggtagaaacg cttggtcctg ttctggaaag
    11881 ccacgaacgt ttcccggagc gcgccaatat cggttttatg caagtggtta agcgcgagca
    11941 tattcgttta cgcgtttatg agcgtggggc aggagaaacc caggcctgcg gcagcggcgc
    12001 gtgtgcggcg gttgcagtag ggattcagca aggtttgctg gccgaagaag tacgcgtgga
    12061 actccccggc ggtcgtcttg atatcgcctg gaaaggtccg ggtcacccgt tatatatgac
    12121 tggcccggcg gtacatgtct acgacggatt tattcatcta tgaagcaacc aggggaagaa
    12181 ctgcaggaaa cactcacgga gcttgatgac cgggcggttg tcgattatct gattaaaaat
    12241 cctgagtttt ttatccgcaa tgcgcgcgca gtagaagcga tacgtgtgcc gcatccggta
    12301 cgcggcaccg tttcgttggt cgagtggcac atggcccgcg cacgtaatca cattcatgtt
    12361 ctggaagaga acatggcgtt gttgatggag caggctatcg ccaacgaagg cctgttttat
    12421 cgcttactct acttgcagcg tagtctcacc gccgccagca gtctcgacga tatgctgatg
    12481 cgctttcacc gctgggcgcg cgatctcggc ctggcgggtg cgagcctgcg cctgttcccg
    12541 gattgctggc gtttaggtgc gccgtcgaac cacactcatc tggcattaag ccgtcagtct
    12601 ttcgaaccgc tgcgtattca gcgtttgggg caggaacagc actatcttgg gccgcttaac
    12661 ggaccggagc tgctggtggt gctaccggaa gcgaaagcgg tgggatcggt ggcgatgtcg
    12721 atgctgggaa gcgatgctga tttgggtgtc gtgctgttta ccagtcgcga tgccagtcac
    12781 tatcaacaag ggcagggaac gcagttactt catgaaattg cgctgatgtt gccggagctt
    12841 ctggagcgtt ggattgaacg cgtatgaccg atttacacac cgatgtagaa cgctacctac
    12901 gttatctgag cgtggagcgt cagcttagcc cgataaccct acttaactat cagcgtcagc
    12961 ttgaggcgat catcaatttt gccagtgaaa acggcctgca aagctggcag caatgtgatg
    13021 tgacgatggt gcgcaatttc gctgtacgca gccgccgtaa agggctggga gctgcaagtc
    13081 tggcgttacg gctttctgcg ctacgtagct tttttgactg gctggtcagc cagaacgaac
    13141 tcaaagccaa cccggcgaaa ggtgtttcgg caccgaaagc gccgcgtcat ctgccgaaaa
    13201 acatcgacgt cgatgatatg aatcggctgc tggatattga tatcaacgat cccctcgccg
    13261 tgcgcgatcg ggcaatgctg gaagtgatgt acggcgcggg tctgcgtctt tccgaactgg
    13321 tggggctcga tatcaaacac ctcgacctgg agtccggcga agtgtgggtg atgggaaaag
    13381 gcagcaaaga gcgccgcctg ccgattggtc gcaacgctgt ggcgtggatt gagcactggc
    13441 ttgatttgcg cgacctgttt ggtagcgaag acgacgcgct ttttctgtcg aaactgggca
    13501 agcgtatctc cgcgcgtaat gtgcagaaac gctttgccga atggggcatc aaacaagggc
    13561 tgaataatca cgttcacccg cataaattac gtcactcgtt cgctacgcat atgctggagt
    13621 cgagcggcga tcttcgtggt gtgcaggagc tgctgggtca tgccaacctc tccaccacgc
    13681 aaatctatac tcatcttgat tttcaacacc ttgcctcggt gtacgatgcg gcgcatccac
    13741 gcgccaaacg ggggaaataa tgcgttttta ccggcctttg gggcgcatct cggcgctcac
    13801 ctttgacctg gatgataccc tttacgataa ccgtccggtg attttgcgca ccgagcgaga
    13861 ggcgcttacc tttgtgcaaa attatcatcc ggcgctgcgc agcttccaga atgaagatct
    13921 gcaacgcctg cgccaggcgg tacgggaagc ggaacccgag atttatcacg acgtgacgcg
    13981 ctggcgtttt cgttcgattg aacaagccat gctcgacgcc gggctgagtg ccgaagaagc
    14041 cagtgcaggc gcacacgcag caatgatcaa ctttgccaaa tggcgcagcc gaatcgatgt
    14101 cccgcagcaa acccacgaca ccttaaaaca gctggcgaag aaatggccgc tggtggcgat
    14161 caccaacggt aacgcccagc cggagctgtt tggtttaggg gattattttg agtttgtgtt
    14221 gcgcgctggc ccgcacgggc gctcaaaacc gttcagcgat atgtacttcc tggcggcgga
    14281 aaaactcaac gtgccgatcg gcgagatctt acatgttggg gacgatctca ccactgacgt
    14341 gggtggggca attcgcagcg gaatgcaggc ctgttggatc agaccggaaa atggcgatct
    14401 gatgcaaacc tgggacagcc gtttactgcc gcatctggaa atttcccggt tggcatctct
    14461 gacctcgctg atataatcag caaatctgta tatataccca gctttttggc ggagggcgtt
    14521 gcgcttctcc gcccaaccta tttttacgcg gcggtgccaa tggacgtttc ttacctgctc
    14581 gacagcctta atgacaaaca gcgcgaagcg gtggccgcgc cacgcagcaa ccttctggtg
    14641 ctggcgggcg cgggcagtgg taagacgcgc gtactggtgc atcgtatcgc ctggctgatg
    14701 agcgtggaaa actgctcgcc atactcgatt atggcggtga cgtttaccaa caaagcggcg
    14761 gcggagatgc gtcatcgtat cgggcaactg atgggcacca gccagggcgg catgtgggtc
    14821 ggcaccttcc acgggctggc gcaccgtctg ctgcgtgcgc accatatgga cgccaatctg
    14881 ccgcaggatt ttcagatcct cgacagcgag gaccagctac gcctgctcaa acgcctaatc
    14941 aaggcgatga acctcgacga gaagcagtgg ccgccgcgcc aggcaatgtg gtacatcaac
    15001 agccagaaag atgaaggcct gcgtccacat catattcaaa gctacggtaa tccggtggag
    15061 cagacctggc agaaggtgta tcaggcgtat caggaagcgt gtgaccgcgc gggcctggtg
    15121 gacttcgccg agctgctgct acgtgctcac gagttgtggc ttaacaagcc gcatatcctt
    15181 cagcactatc gcgaacggtt taccaatatc ctggtggatg aattccagga taccaacaac
    15241 attcagtatg cgtggatccg cctgctggcg ggcgataccg gcaaagtgat gatcgttggc
    15301 gatgacgacc agtcaatcta cggctggcgt ggggcgcagg tcgagaatat tcagcgtttc
    15361 ctcaatgatt tccccggtgc tgaaactatc cgtctggagc agaactaccg ctctaccagc
    15421 aatattctga gcgccgctaa cgccctgatt gaaaacaata acgggcgtct gggtaaaaaa
    15481 ctgtggaccg atggcgcgga cggtgagcct atttccctct attgcgcttt taacgaactc
    15541 gacgaagcgc gttttgtggt taaccgtatc aagacctggc aggacaatgg cggggcgctt
    15601 gccgagtgcg ccattctcta ccgcagcaac gcccagtcgc gtgtactgga agaagcactg
    15661 ttgcaggcga gtatgccgta ccgtatttac ggcgggatgc gcttcttcga acgccaggaa
    15721 atcaaagatg cgctctcgta tctgcgcctg attgccaacc gtaatgacga cgcggcgttt
    15781 gagcgcgtgg tgaatacacc aacgcggggt attggtgacc ggacgctgga cgtggtacgt
    15841 cagacatcgc gcgatcgcca gttaacactc tggcaggcat gtcgtgaact gttgcaggaa
    15901 aaagccctcg ccggacgtgc tgccagtgcc ttacagcggt ttatggaact gatcgacgcc
    15961 ttagcgcagg aaactgccga tatgccgctg catgtacaga ctgaccgggt aattaaagac
    16021 tccggcctgc gcacaatgta cgagcaggag aaaggcgaaa aaggccagac gcgtatcgaa
    16081 aacttagagg aactggtaac ggcgacgcgc cagttcagct acaacgaaga agacgaagat
    16141 ttaatgccgc tgcaggcgtt cctctcccat gcggcactgg aagcgggcga ggggcaggcg
    16201 gatacctggc aggatgcggt gcagttgatg acgctacact cggcgaaagg tctggaattc
    16261 ccgcaggtgt ttatcgttgg tatggaagag ggcatgttcc caagccagat gtcgctggat
    16321 gaaggcgggc gtctggaaga agaacgccgt ctggcctacg ttggcgtaac ccgcgcgatg
    16381 cagaaactga cgctgaccta cgcggaaacc cgccgtctat atggcaaaga ggtttaccat
    16441 cgcccgtcgc gctttatcgg tgagttgccg gaagagtgtg tggaagaggt gcgcctgcgc
    16501 gccacggtaa gccgcccggt cagccatcaa cggatgggga cgccgatggt cgagaacgac
    16561 agcggctaca agctcggcca gcgcgtacgc cacgctaagt ttggtgaagg caccatcgtc
    16621 aatatggaag gcagcggtga gcatagccgt ttgcaggtgg cgttccaggg ccagggtatt
    16681 aaatggctgg tggcggctta cgcccggttg gagacggtgt gacgttgcgg gatgcggtgc
    16741 gccacaccgc attccgcatc agcaccttac ttagcgcact tcttcacatc aatattccag
    16801 tcagcaccag acgcaacctg atgtttctgc gcaaaattat ccatattcag cgccacggaa
    16861 acattcaata agctgttcag atacaccatc ggctggcctt ccggcacatc gccgaagctt
    16921 gcgacatacg gcattttccc tgcgtatttt tgctgggaac cttcggaaat ggtcacacac
    16981 aacgtgtcgt tgagtttgat tcctgcctgt ttcagcaact cgtcgctgat attgctccag
    17041 acgttgccgt actgaatatc gagaatcggg atattgccct tcacttcacc ttttgttgct
    17101 tttgctttct gataagaaag ttcgacaact tttgcaggaa gctctggccc cacctgttcg
    17161 aacgtaatcg ctccggaagc cagacgcgca ccagtgtagg catatacatc gcgaccatgg
    17221 aaggtatatg acttttcaga acctttcagg cggttagctt tttcgtcgat ttcccggacg
    17281 ctatcaatac ccagagtttg tgccaccaaa gtgagcgtac cgttatctgg tgaaacgaag
    17341 tattgaccgt ttttggtttt cagcactacc gatttacggt cagtgcctac gcccggatcg
    17401 accacgctaa caaacaccga accttgcggc cagtaactgg cagtctgata caggcgataa
    17461 gcgccttccc agatgttata cggtggaatt tcgtgtgtta aatcaaagat tttaagatta
    17521 tggtcaacgc cgaatgcgac ccctttcatc gccgagacgg caccatcttt caggctaaaa
    17581 tccgtttgta aaatcaaagc gttatccgca taagcggatg atgaccccag acaggttaat
    17641 aaaaataatg ttgcgagcgt ttttttcatt tttgtttccc ttcatttatc tgatgatttt
    17701 ccagctgtgt agccagcccc agtttgtctg gcctcctcga gtgtacaatg tttaaccttt
    17761 ctgctcgacg gaaatcatgg tgacaaaagg atagcgttgc cacgggattg cctcgccttt
    17821 catatacata tgtgaaattg tgccatcaag ataaagcagc tgcccaacgt tcagtttcgc
    17881 tttggcataa caggcaaaat cataaaaatt tgtcgcctgc tggctcaaca aaaacacggc
    17941 gttcccatgt ttattaatcc caacaccgtt acgaatttta cgtgaggcga cgttgggatg
    18001 aatacgcgga ttaattacac cgttttccat caacaatggc cctgactgca ccgcaaactg
    18061 aatctcttta ctggttttaa aggcatccag acgaacgatg ccgactttat ctcccgcgac
    18121 ataaaacact ccgccaggac gaataaagaa attcccttca cctgaagcga gatttaacgc
    18181 caccttctgc tgaccgtttt cgatgtacaa accgagcggc gcatagcttt catcatagat
    18241 gccgccgttc atcgccatct gcacctgacc ctgactatta atatccgcca gcagagcatg
    18301 taacgttccc cacgcttcgc cattggcttt ttgccagtac attttcaccc gctctgtttg
    18361 aggattaacg gtatacgcct gtacggtcag cgtcggatct gagggtgcgc aatcatcagc
    18421 ggcaacagca aacaagggaa gtaaggtgag ggtgagaaaa atccgtttga gattcaaggt
    18481 gatcattcct ttaccaatga atagccgatg cgccattata ggccctggat gtgggatttt
    18541 tttatcctgt tagcgacctt gacgagtacc aaaaagcgcg aagttcaact attgttctgt
    18601 ggtgttctgt tgcgtgttga cggcaaaatt ttgctggcgt aacatgcgcg cacgatcact
    18661 ctaagaggac attcgccttg gacacaccca gtagatactg gctcactatc ctgtcatcca
    18721 ggatcaactc ctaaggctat ccctttttgc tgatagcctt agcggttgtc agcgacctca
    18781 atttttcccg tcgcgctgag tcaggctgtt taatggtctg aaacccaatt tgtttctgtg
    18841 tgcccaccga actgtccgat attttaagca ttgggagtcc cggtcatgct gagcgcattt
    18901 caactggaaa ataaccgact gacccggctg gaagtcgaag agtcacaacc ccttgtaaat
    18961 gcagtatgga ttgatcttgt cgaaccggac gacgacgagc gactgcgcgt acaatctgaa
    19021 cttggtcaga gcctggcaac ccgcccggaa ctggaagaca tcgaagcatc ggcacgtttc
    19081 tttgaagacg atgacggcct gcatattcac tccttcttct tctttgaaga tgcggaagat
    19141 cacgccggta actctactgt ggcatttacc atccgtgatg gtcgtctgtt tactctgcgt
    19201 gagcgtgaac tgcctgcatt ccgtctgtat cgtatgcgcg cccgtagcca gtcaatggtg
    19261 gacggtaacg cctacgagct gctgttggat ctgttcgaaa ccaaaatcga acagttggcg
    19321 gatgaaattg aaaacatcta cagcgatctg gagcagttga gccgggtgat tatggaaggg
    19381 catcagggcg atgagtacga cgaggcgctc tccacgctgg cggaactgga agatatcggc
    19441 tggaaagttc gcctgtgtct gatggatacc cagcgtgcgc tcaacttcct ggtacgtaaa
    19501 gcacgcttac cgggtgggca actggagcag gcgcgtgaaa tcctgcgaga tatcgaatcc
    19561 ctgctgccgc ataacgaatc cctgttccag aaggtgaact tcctgatgca ggcggcgatg
    19621 ggctttatca atatcgagca gaaccgcatc atcaaaatct tctcggtggt atccgtggtg
    19681 ttcctgccac cgacgctggt cgcttccagc tacgggatga actttgagtt tatgccggaa
    19741 ctgaagtgga gcttcggcta cccaggcgcg attatcttta tgatcctcgc gggcctggca
    19801 ccgtatctgt actttaagcg gaagaactgg ttgtaatata aatgccgggt aaagattgtt
    19861 ctttatccgg cttattgctg aaaattgtct ttttcaaagt catgcgggtt gaacactcgc
    19921 tctatcttat tttcctgggt tagttgatac tcattgttat cag
//
LOCUS       DAZUCC010000077        19921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZUCC010000077 DAZUCC010000000
VERSION     DAZUCC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.077"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            37..2427
                     /gene="gcd"
                     /locus_tag="ACHY09_002416"
     CDS             37..2427
                     /gene="gcd"
                     /locus_tag="ACHY09_002416"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFS3885378.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILAWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKNLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2623..4173)
                     /gene="cueO"
                     /locus_tag="ACHY09_002417"
     CDS             complement(2623..4173)
                     /gene="cueO"
                     /locus_tag="ACHY09_002417"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFS3885379.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     VIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4339..4686
                     /gene="yacC"
                     /locus_tag="ACHY09_002418"
     CDS             4339..4686
                     /gene="yacC"
                     /locus_tag="ACHY09_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFS3885380.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4792..5658
                     /gene="speE"
                     /locus_tag="ACHY09_002419"
     CDS             4792..5658
                     /gene="speE"
                     /locus_tag="ACHY09_002419"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFS3885381.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            5674..6468
                     /gene="speD"
                     /locus_tag="ACHY09_002420"
     CDS             5674..6468
                     /gene="speD"
                     /locus_tag="ACHY09_002420"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFS3885382.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITTALWKEMREIYYGRNMPAV"
     gene            complement(6506..6868)
                     /gene="yacL"
                     /locus_tag="ACHY09_002421"
     CDS             complement(6506..6868)
                     /gene="yacL"
                     /locus_tag="ACHY09_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFS3885383.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(7043..9640)
                     /gene="acnB"
                     /locus_tag="ACHY09_002422"
     CDS             complement(7043..9640)
                     /gene="acnB"
                     /locus_tag="ACHY09_002422"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFS3885384.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            9995..11752
                     /gene="yacH"
                     /locus_tag="ACHY09_002423"
     CDS             9995..11752
                     /gene="yacH"
                     /locus_tag="ACHY09_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFS3885385.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQEVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPTNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHSAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSDHTVRLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(11823..13247)
                     /gene="lpdA"
                     /locus_tag="ACHY09_002424"
     CDS             complement(11823..13247)
                     /gene="lpdA"
                     /locus_tag="ACHY09_002424"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS3885386.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13572..15464)
                     /gene="aceF"
                     /locus_tag="ACHY09_002425"
     CDS             complement(13572..15464)
                     /gene="aceF"
                     /locus_tag="ACHY09_002425"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS3885387.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKDVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(15479..18142)
                     /gene="aceE"
                     /locus_tag="ACHY09_002426"
     CDS             complement(15479..18142)
                     /gene="aceE"
                     /locus_tag="ACHY09_002426"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFS3885388.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18303..19067)
                     /gene="pdhR"
                     /locus_tag="ACHY09_002427"
     CDS             complement(18303..19067)
                     /gene="pdhR"
                     /locus_tag="ACHY09_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFS3885389.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(19523..19813)
                     /locus_tag="ACHY09_002428"
     CDS             complement(19523..19813)
                     /locus_tag="ACHY09_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS3885390.1"
                     /translation="MYNFKDKVEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDKPENILKIVKEWRRSQGLSLFKDSE"
BASE COUNT         4637 a         5454 c         4938 g         4892 t
ORIGIN      
        1 tactaaaata ttaatgaatt gaaatggtgt ctctttatgg caattaacaa taccggctcg
       61 cggcgattac tcgtcacgct aacagccctt tttgcagcgc tttgcgggct gtatctactc
      121 attggcggag gctggctggt cgcgattggc ggctcctggt actaccctat cgctggcctt
      181 gtgatgctcg gcgtcgcctg gatgctgtgg cgcagtaaac gcgccgcgct ttggctgtac
      241 gcagccctgc tgctcggcac tatgatttgg ggcgtctggg aagttggttt cgacttctgg
      301 gcgctgactc cgcgcagcga cattctggtc ttcttcggca tctggctgat cctgccgttt
      361 gtctggcgtc gcctggtcat tcctgccagc ggcgcagttg ccgcactggt ggtggcactg
      421 ctgattagcg gtggtatcct ggcctgggcc ggatttaacg atccgcagga aatcagcggc
      481 accttaagcg ccgataccac gccagcagaa gccatctccc ccgttgccga tcaggactgg
      541 cctgcctatg gtcgcaatca ggaaggtcaa cgcttttcac ctctgaaaca aattaacgct
      601 gataacgtcc acaagctgaa agaagcctgg gtgttccgta ctggcgatgt gaagcagccg
      661 aacgatccag gtgaaatcac caatgaagtg acgccaatta aagtgggcga caccctttac
      721 ctgtgtaccg ctcaccagcg cctgtttgcg ctcgatgccg ccagcggcaa agagaaatgg
      781 cattacgatc ctgagctgaa aaccaacgag tccttccagc atgtaacctg ccgtggcgtc
      841 tcttatcatg aagccaaagc agaaactgct tcgccggaag tgatggcgga ttgcccgcgt
      901 cgtatcattc ttccggtcaa tgatggtcgt ctgattgcaa ttaacgctga aaacggcaag
      961 ctgtgcgaaa ccttcgccaa taaaggcgtg ctcaatctgc aaagcaatat gccggacacc
     1021 aaaccgggtc tgtatgaacc gacttcgccg ccgattatca ccgataaaac catcgtgatg
     1081 gccggttcag tcaccgataa cttctcaacc cgtgaaacat ctggcgtgat ccgtggtttt
     1141 gatgtcaaca ccggggagct gctgtgggct tttgatcctg gtgcgaaaga tcctaacgca
     1201 atcccgtctg acgaacacac ctttaccttt aactcgccaa actcgtgggc accagcggcc
     1261 tatgacgcga agctggatct ggtgtatctg ccgatgggcg tgaccacgcc ggatatctgg
     1321 ggcggtaacc gcacaccgga acaggaacgt tatgccagct cgattctggc gctgaatgcc
     1381 acaaccggga aactggcgtg gagctaccag accgttcacc acgacctgtg ggatatggat
     1441 cttccggcac agccgacgct ggcggatatc accgttaatg gtcagaaagt gccagttatt
     1501 tatgctccgg cgaaaaccgg caacattttt gtgctcgatc gtcgtaatgg cgaactggtg
     1561 gttccggcac cggaaaaacc ggttccccaa ggtgctgcca aaggcgatta cgtaacccca
     1621 actcaaccgt tttctgaact gagcttccgt ccgacgaaaa atttgagcgg tgcggatatg
     1681 tggggagcca ccatgtttga ccagctggtg tgccgcgtga tgttccacca gatgcgctat
     1741 gaaggcattt tcaccccgcc atctgaacag ggtacgctgg tcttccctgg taacctgggg
     1801 atgttcgaat ggggcgggat ttccgttgat ccgaatcgtg aagtggcaat tgccaaccca
     1861 atggcactgc cgtttgtttc gaaactgatc ccacgcggtc ctggcaaccc gatggagcag
     1921 ccgaaagatg ccaaaggcac gggtacggaa tccggcattc agccgcagta cggtgtaccg
     1981 tatggcgtca cgctcaatcc gttcctctca ccgtttggtc tgccgtgtaa acagccagca
     2041 tggggttata tctcggcgct ggatctgaaa accaatgaag tggtgtggaa gaaacgtatt
     2101 ggtacgccgc aggacagtat gccattcccg atgccggtac ctgtgccgtt caatatgggt
     2161 atgccgatgc tgggcgggcc aatctccacg gcgggtaacg tgctgtttat cgccgctacg
     2221 gcagataact acctgcgcgc ttataacatg agcaacggtg aaaaactgtg gcaaggtcgt
     2281 ttaccagcgg gtggtcaggc gacgccaatg acctatgaag tgaatggtaa gcagtatgtg
     2341 gtgatctccg caggcggtca cggttcattt ggtacgaaga tgggcgacta tattgtggct
     2401 tatgcgttgc cggatgatgt gaagtaaggc ttgctcagat tgctgacaac gtgcgatttg
     2461 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaacatttgc aaattcaata
     2521 cattgcgcgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg tcatcagttt
     2581 catgcccgga gagatccggg catatttccg aatacggtct ttttataccg taaaccctaa
     2641 catcatcccc gtatcttcat gctccagcaa atggcagtgc gccatataag catgttcttt
     2701 cggtgcatcg tgattaaact tcaccagcac ttcgctgaca ttaccttcta ccttaacggt
     2761 atctttccag cctgcgcgat gcgtcgctgg cggtttgcca ttttctgaca agatacggaa
     2821 ctgcgtaccg tggatatgga acggatgcag catcatgtcg cccacgccag agataaccca
     2881 acgttcgtat tgccctttcg ccgccgcaaa catcggcttg ttcatatcaa acgcctgacc
     2941 gttgatttta ttggcatggt ggaaatcgaa cttcccgccg tggttcatat gattcatatt
     3001 gccgtgcccc atatggccca tcatctggct gtgatccatc ccggccatcg cctgatcgcc
     3061 atatttctcc atcagcatct gcatccccat catatcgagc attgggtcca tagaaagttg
     3121 cagcttgcgt accgtcagcc cttccagcga aggtaacgca ggcaggctac ttaatgtgtc
     3181 tggcaaagca ccagaggcac taatggcaat cggctgaatc cgcattaccg gatgcggctt
     3241 gtcaaacggc gcaatcgcca tccccatctg gctgaccggc agcgtcacca ggtcaaaggg
     3301 tttgttgtcg ttaacctcca ccagcacttc aaaacgctcg cccatcagca ctggcagctc
     3361 gttcaccttc accggttcag gtagcagacc gccgtcgctg gcaatcacat aaagcgggcg
     3421 attgtcgctg gtggcgaaat tgagcgagcg ggcattacag ccattgagca aacgcaggcg
     3481 cagccagcca cgcggggcag cgtgttgtgg gtagattaca ccgttggtca gcaacgtatc
     3541 gccaaaccaa cccacagcgg cggtcatcac atccagttga taatcaatct gcccgtcagc
     3601 gttaaatttt ttatcctgaa caataaccgg gacatcatca ataccccact gttttggcag
     3661 cattaatttc aggatctcgt catcttcaat caccaccaaa ccagccagcc ccatcgccac
     3721 ctgacgtccg gtcttgccat gttgatgtgg atggaaccag caggtagcgg caggttgatc
     3781 aacgttcaac gtcaccgagc gcttgccacc tggcggaata attccctgcg ggccgccgtc
     3841 cacttcaccc ggtacttcca gcccgtgcca gtgcaacgtc gtctcttccg tcagttggtt
     3901 gtagatatca accgttaccg ctttgccgcg ctgtaatttc accgccggcc ccagcagatt
     3961 gccgttatag ccccaggtag ttgcggtttt cccgccaaag gtggactgac ctgcgccaat
     4021 agttaactga atgcgattac gggcatcggt cgtgagcaaa tcagggatcg gtaacgttgg
     4081 gcgttccgcc gcaaatactg cgcggctcca cagcggcaaa gccgaagcca cgcccagcgc
     4141 gacggaatat tttaagaaat cacgacgttg catagttatt tccttattct taagcaggcg
     4201 aaacaaaatc aaacgttgag catagtcctt ccccttacgg gaaggtcaag ccgcagacac
     4261 aatttaatcg acaataatgg tcgtcgcctc gctcgcagtg tgctaacgtt tatcttcttt
     4321 aagcccctgg tagaagcaat gaagacgttt ttcagaacag tgttattcgg cagcctgatg
     4381 gccgtctgcg caaacagtta cgcgctcagc gagtctgaag ccgaagatat ggccgattta
     4441 acggcagttt ttgtttttct gaagaacgat tgtggttacc agaacttacc taacgggcaa
     4501 attcgtcgcg cactggtctt ttttgcccag caaaaccagt gggatctcag taattacgac
     4561 accttcgaca tgaaagccct cggtgaagac agctaccgcg atctcagcgg cattggcatt
     4621 cccgtcgcta aaaagtgcaa agccctggcc cgcgattcct taagcctgct tgcctacgtc
     4681 aaataatccc tgataccttt ttgtagaaat attgaccgtg catccgcggt caatgttagc
     4741 tattatgttg cgcccttttt ttacgggtgt taacaaagga ggtatcaacc catggccgaa
     4801 aaaaaacagt ggcatgaaac gctacacgac cagtttgggc agtactttgc ggtagataac
     4861 gttctgtatc atgaaaagac cgatcaccag gatctgatca tttttgagaa cgccgcattt
     4921 ggtcgcgtaa tggcgctgga tggcgtagta caaaccaccg agcgcgacga gtttatctat
     4981 catgagatga tgacccatgt tccgctactg gcccacggtc acgcgaaaca tgtgctgatt
     5041 atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc gacataaaaa cgttgagtca
     5101 atcacgatgg tggaaatcga tgcgggtgtc gtgtcgttct gccgtcagta tctacccaac
     5161 cataacgccg gtagctacga cgatccgcgc tttaaactgg tgatcgacga tggcgtcaat
     5221 ttcgttaatc aaaccagcca gacctttgat gtcattattt ccgactgcac cgatcctatc
     5281 ggtcccggcg aaagtctgtt cacctcagca ttttatgaag gctgcaaacg ttgcctgaat
     5341 cctggcggta tcttcgtcgc acaaaacggc gtctgctttt tacagcagga agaggccatc
     5401 gacagccatc gcaaactcag ccattacttc agcgatgttg gcttttatca ggcggcgatc
     5461 ccgacctatt acggcggcat catgaccttt gcatgggcga cagataacga cgccttacgc
     5521 catctctcaa ccgaaattat tcaggcgcgt tttctcgctt ctggcctgaa atgccgttat
     5581 tacaatccgg cagtccatac ggcagctttt gccttacccc agtatctgca agatgcactg
     5641 gcttcacagc cgtcctaagg agaagataag aaattgaaaa aactgaaact gcatggcttt
     5701 aataatctga ccaaaagtct gagtttttgt atttacgata tctgctacgc caaaactacc
     5761 gaagagcgcg acggttatat tgcttatatc gatgaactct ataatgccaa ccgtctgacc
     5821 gaaatcctgt cagaaacctg ttccattatc ggggccaata ttcttaacat cgcccgccag
     5881 gattacgagc cacaaggtgc cagcgtcact attctggtga gtgaagaacc ggtcgacccg
     5941 aaacttatcg acaaaacaga acaccccggc ccactgccag aaacggtcgt tgcccatctc
     6001 gataaaagtc atatttgcgt acatacctac ccggaaagtc atcctgaagg cggtttatgt
     6061 accttccgcg ccgatattga agtctctacc tgcggcgtga tttctccgct gaaggcgctg
     6121 aattacctga tccaccagct tgagtccgat attgtgacta ttgattatcg cgtgcgcggt
     6181 tttacccgcg acattaacgg tatgaagcac tttatcgacc atgagattaa ttcgattcag
     6241 aactttatgt ctgacgatat gaaggcgctg tatgacatgg tggatgtaaa cgtctatcag
     6301 gaaaatatct tccataccaa gatgttgctt aaagagttcg accttaagca ctacatgttc
     6361 cacaccaaac cggaagattt aaccgacagc gagcgccagg aaattaccac tgcgctgtgg
     6421 aaagaaatgc gcgagattta ttacgggcgc aatatgcccg ctgtttaacg tctcaggcgg
     6481 agcacggagg ctccgcctga tttacttact tctgctgcac gaaattacgg taagccgcca
     6541 ccacctgaag aaaatcctca acgccgcaca gcgacaagct ctcttcatcg tagtagttca
     6601 tcccctcttc catttcatcg ccagcgaatt ccagttgatt ggcgcgaacc atcacctctt
     6661 caccatccat ccatagcgtg tattcatgcc ctgcccgttg ccaggaacgt tcgctacctt
     6721 tcaatgcgtg cgccgcttgt tctacttcat caagcaaggc caggttttct ttcacctctt
     6781 cattaaacca gtgcccgacc acttcatgcc ccatggacat gcgcaccttt accactccgg
     6841 taatatcgcg cagaaattcg taatccatag tgtgttcctc tgctcctggc aatgcgccga
     6901 aatcgcattg cgccattgat gtaattatcg cagccttacc ggagaaaaac agcggaacgg
     6961 cgatggttta gaaataaaaa atgcccggga atctcattca ccgggcattg tgtcgtttat
     7021 gcgcagcgcg tgcgcttact tattaaaccg cagtctggaa aatcaccccg tccgcttttt
     7081 cggtgtactg agaaagctgg tcgaagttca gataacggta agtatcaacg gctgttttat
     7141 ctacctgcgc cacgtaggtc tggtactctt ccggcgtcgg cagtttgcca atcagcgccg
     7201 caacagcagc cagttccgca gaagccagga agacattcgc gccagtaccc aaacggttcg
     7261 ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac
     7321 ccatacacag ggaacagcca gggatctcga tacgagcacc actcttaccg aagacgctgt
     7381 agtagccttc ttcggtcagc tgcgcagcgt ccatacgcgt tggcggtgcc acccacaggc
     7441 gggtcggcaa ctgacctttg tgcgcatcca gcagtttacc agcagcacgg aagtgaccga
     7501 tgttagtcat gcaagaaccg ataaatactt cgtcgatctt ctcgccctgt accgcagaca
     7561 gcggacgtgc gtcgtccggg tcattcggtg cacagaggat tggctctttg atatccgcca
     7621 gatcgatgtc gatcactgcc gcgtattccg catctgcatc ggcttccagc agctcaggat
     7681 tcgccagcca tttttccatg ccctgaatac gacgctccag ggtacgacga tcgccgtaac
     7741 cttccgcgat catccacttc agcaggacga tgttagagtt caggtattca atgatcggtt
     7801 ctttgttcag cttgatggta caaccggcag cagaacgctc ggcagacgca tcggtcagct
     7861 caaatgcctg ctcaactttc agatccggca gaccttcaat ttccagaatg cggccagaga
     7921 agatgttttt cttacctttc ttctcaacgg tcagcagacc ttgtttgatt gcatacagcg
     7981 ggatcgcgtg taccagatcg cgcagggtga tacccggctg cattttgcct ttgaagcgca
     8041 ccagaacgga ttctggcata tccagcggca ttacgcctgt tgcggctgca aatgccacca
     8101 gaccagaacc cgccgggaaa gagataccga tcgggaaacg ggtatgggag tcaccaccgg
     8161 taccgacagt atccggcagc agcatacggt tcagccagga gtggattacg ccgtcacccg
     8221 gacgcagcga cacaccgcca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttaa
     8281 cgtcaactgg tttcggatac gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg
     8341 agaagcccag gcacgccagg tctttcagtt catcacgggt catcgggccg gtggtgtcct
     8401 gggaacctac agaagtcatt ttcggttcac agtacgcgcc cggacgaatg cctttcacgc
     8461 cacaggcacg gcctaccatt ttctgcgcca gcgagaagcc gcgatcgctc tcagcgacat
     8521 ctttcgcctg acggaacaca tcgctgtgcg gcaggccaag tgcttcacgc gctttggtgg
     8581 tcaggccacg cccaataatc agcggaatac ggccgccagc acgcacttca tcaatcagca
     8641 cgtcggtttt cagttcgaag gtcgccagca gttcgccggt ttcgtggtta cgcacttcac
     8701 ctttgtacgg gtaaacgtca attacgtcgc ccatgttcag gttagagacg tcgacttcga
     8761 ttggcagcgc acccgcgtct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga
     8821 ggcacaaacc accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca
     8881 gaacggagtt agtggcggat ttacgcgatg accccgtacc cacaacgtca ccgacgtacg
     8941 ccagtgggaa acctttctgt tgcagagctt cgatttgctt aatcggacca acaacgcctg
     9001 gctgatctgg ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga
     9061 tatccgggcg tgaccacgca tccggtgccg gagagaggtc atcggtgttg gtttcgcctg
     9121 tgactttgaa gacggtaacg gtcagttttt cagccagcgc cgggcgattc aggaaccatt
     9181 cggcatccgc ccaggactgc attacctgct tcgcatattc gttgcctgct ttcgctttct
     9241 cttctacgtc atagaagtta tcgaacatca gcagcgtgtg agaaagtgct ttggcagcga
     9301 tcggtgccag tttggcatca tccagcgcgt cgatcagcgg atgaatgttg taaccaccct
     9361 gcatggtgcc cagcagttcg atggcttttt ccggagtcag cagaggggat ttggcttcgc
     9421 ctttagcgat agcagccagg aagcctgctt ttacataggc ggcttcatcg acgcctgggg
     9481 gaacacggtt ggttaacaga tctaacagga attcttcttc gcccgcgggc gggtttttca
     9541 gcagctctac aagcgcggcc atttggtttg catccagggg tttgggcgca atcccctcag
     9601 cggcacgctc agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca
     9661 ttgtcatagt gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta
     9721 aatgcccagt accgcaacgg gcagaatagc aggattttga cgatgtgtta atctgtttac
     9781 aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc
     9841 cgtaaaaact tcgttaattc gcatggtgat aatcaccttt ctcaacgaat cccaagatta
     9901 gtcaaaattt aaactaccgc ctctttatac tcggattcac agcacctgcg ggtggcagtt
     9961 cgccgaccat tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc
    10021 ccatgtgctg gcgctaattt gcagtgccgg gctttgtgcc gcctctaccg ggctatatat
    10081 aaaaagccgc acagtggaag cgcctgtgga accgcaatcg acacaactgg ctgcgcctga
    10141 catctccgca gttacgcttc ctgcaacggt ttccgtgcct cctgtaacac ctgccgtcgt
    10201 caaatccgca ttcagcactg cgcaaataga tcaatgggtt gcacctgtcg cgctataccc
    10261 cgactctctg ctttcacaag tgttaatggc atcaacctat ccggcaaacg ttgctcaagc
    10321 agtgcaatgg tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc
    10381 tgaccagccc tgggacgcca gcgtcaaatc actggtggcc tttccacagt tgatggcatt
    10441 gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca
    10501 ggaggtgatg gactcggtac agcgattgcg gcaactggcg caacaaaccg gctcgctgaa
    10561 gtcatcaact gaacagaaag ttattaccac aacgaagaaa gctgtaccgg taaaacagac
    10621 agtcacggct cccgtcatac catccaatac cgtttcaact gccaaccctg tcattacaga
    10681 gcctgcaaca accgtcattt ccattgaacc caccaatcct gatgtggtct atattcccaa
    10741 ctacaaccca accgtggttt acgggaactg ggccaatact gcgtatccgc cggtttatct
    10801 gccgccgcca gccggagaac cgtttgttga cagctttgtg cgcggattcg gctatagcat
    10861 gggcgttgct accacgtacg cgctattcag cagcatcgac tgggatgacg acgatcatga
    10921 ccatcatcat catgacgatg atgattatca ccatcacgat ggcggtcatc gtgatggtaa
    10981 tggctggcaa cacaatggcg acaacatcaa tatcgacgtc aacaatttca accgtatcac
    11041 cggtgaacat cttactgata agaatatggc atggcggcac aatccaaact accgtaatgg
    11101 tgtgccctat catgatcagg atatggcaaa gcggtttcat caaaccgatg tcaacggcgg
    11161 aatgagcgcc acgcagctac ctgctccaac acgcgacagc cagcgtcagg cggcagcaag
    11221 tcagtttcag caacgaacac acagcgcacc agtcattaca cgagataccc aacgtcaggc
    11281 agcggcacag cggtttaatg aagctgaaaa ctatgggagc tatgacgact tccgcgactt
    11341 cagccgtcgc caaccactga cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc
    11401 ggcctctcct gagcagcgcc aggcagtccg cgagaaaatg cagactaacc cgcagatcca
    11461 gcagcgaaga gaggcagcgc gtgagcgcat tcagtctgca actcccgaac agcgccaggt
    11521 gtttaaggaa aaagtacagc agcgcccact gaatcaacag caacgtgata acgcccgcca
    11581 gcgtgttcaa tcagcatcac ctgagcaacg tcaggttttt cgggagaaag ttcaggagag
    11641 ccgcccgcaa cgtctaaacg acagtgacca tactgtcagg ctgaataacg agcaacggtc
    11701 agcagtacgc gaacgtctct ctgagcgcgg agcaaggcga ctggaaaggt aaattacaga
    11761 cgtaaaaaaa gcggcgtggt tagccgcttt tttaattgcc agatgttccg gcaaacgaaa
    11821 aattacttct tcttcgcttt cgggttcggc aggtcggtaa tgctaccttc gaacacttct
    11881 gccgccaggc ccacagactc gtgcagagtc gggtgcgcat ggatggtcag tgcgatgtct
    11941 tcagcgtcac aacccatttc gattgccagg ccgatttcac ccagcaactc gccgccgtta
    12001 gtgccgacaa tcgcaccacc gatcacacgg tgagattctt tgtcgaaaat cagcttggtc
    12061 ataccgtctg cgcagtcgga agcgatagca cgaccagaag cagcccacgg gaaggtggcg
    12121 gtttcgtagc tgatgccttt ctctttcgct tctttctcag tcagacctac ccatgcaact
    12181 tctggttcgg tataggcgat ggacgggata actttcggat cgaagtaatg ttttttaccg
    12241 gcgataactt cagcggcaac gtgaccttcg tgaacacctt tgtgtgccag cattggctga
    12301 ccgacgatat caccgatagc aaagatgtgc ggtacgttgg tacgcagctg tttgtcaacg
    12361 cggatgaaac cacggtcgtc aacttccacg ccagctttgc ctgcgtcgag gtttttaccg
    12421 ttcggcacac gaccaatcgc taccagcacg gcgtcgtaac gctgcggttc agcgggtgct
    12481 tttttgcctt ccatcgtcac ataaatgccg tcttctttcg cttcaacggc ggtaactttg
    12541 gtttccagca tcaggttgaa tttcttgctg atacgcttgg taaagacttt aacgatgtct
    12601 ttatcagctg ccgggatgac ctggtcgaac atttcaacca cgtcaatctg tgaacccagc
    12661 gcgtggtata cggtgcccat ttccagaccg atgataccgc cacccattac cagcaggcgt
    12721 tctggtactt ctttcagttc cagcgcgtca gtggagtccc agatacgcgg atcttcatgc
    12781 ggaataaacg gcagttgaat cgggcgagaa cccgctgcaa tgatcgcgtt gtcgaagttg
    12841 atcacggttt taccgttctc accttcaact tccagggtgt tagccccggt aaatttaccc
    12901 agaccgttga ccactttgac tttgcggcct ttcgccatac cagccagacc accggtcagc
    12961 tgattgatca ctttctcttt ccaggtacga atcttgtcga tatcggtttt cggttcgcca
    13021 aagacgatac cgtgttcagc cagcgctttg gcttcttcga taacttttgc tacgtgcagc
    13081 agtgctttag aagggataca gccgacgttc aggcaaacac cgccaagggt gttgtagcgt
    13141 tctacgatta cggtttccag acctaaatca gcgcaacgga aggcagcgga gtaacctgcg
    13201 gggcctgccc caagtaccac gacctgagtt ttgatttcag tactcatcat gacctctata
    13261 tatttatctc cggcggtcat acccgtcgtc tttcaggccg cagcggcgtt agcttcacgc
    13321 cctcacccca gtcacttact tatgtaagct cctggggatt ccggcgcttg ctaccttgct
    13381 gcacccagaa atccataggg tatgtgaagt aataccctaa ccaccaccgg gtcgttctat
    13441 ccgtcggtat tttacaaaat tgttaacaat ttttaaacaa caaacggcaa ccgatttgtc
    13501 tattcgctaa taacctcaat acattcatga gattaccaga aaaaagccgg ccgttgggcc
    13561 ggctctttta cttacatcac cagacggcga atgtcagaca gcgtgttgtt aatgatggta
    13621 atgaaacggg caccatcagc accgtcgatc acgcggtggt cgaaggagag agaaatcggc
    13681 agcatcagac gcggcacgaa ctctttacca ttccacaccg gctccatcgc ggacttggaa
    13741 acgccgagga tagccacttc cggcgcgttc acaatcggcg cgaagtgggt agtacccagg
    13801 ccgccgatgc tggagatggt gaagcaaccg ccctgcattt cgcccgcagt cagcttaccg
    13861 tcacgcgctt tcttagaaat agtcatcagc tcgcgagaca gctcgatgat gcctttcttg
    13921 ttgacgtctt tgaataccgg aacaaccaga ccgttcgggg tatccaccgc cacaccgatg
    13981 ttgatgtatt tcttcagggt cagacgctga ccgtcttccg acagcgaact gttgaagcga
    14041 ggcatctgct caagagctgc agcaacggct ttcatgatga agacaaccgg ggtgatcttc
    14101 acatccagct tacgtttcgc cgcttcttcg ttctgctgtt tacggaacgc ttccagctcg
    14161 gtgatatcgg ttttgtcgaa gtgggtaaca tgcgggatca tcacccagtt acggctcagg
    14221 ttcgcaccag agattttctg gatacggccc agttccactt cttcgatttc accaaacttg
    14281 ctgaagtcca ccttcggcca cggcagcatg cccgggatac cgccgccagt cgccgccgga
    14341 gccgcttctg cacgtttgat agcttctttc acgtaagcct gaacgtcttc gcgcaggata
    14401 cgacctttac ggccagtgcc cttcactttc gccaggttaa cgccaaattc gcgtgccaga
    14461 cggcggatca gcggagtcgc gtgaacgtaa gcgtcgtttt cagcaaattc agatttgcct
    14521 tcggctttcg cagccggagc tgctgctgga gcttcagctt ttgctgccgg agccggcgct
    14581 gccgcttcct gtttcgcagg agctgccgca ggcgctgcgc cttcaacttc gaagatcata
    14641 atcagcgagc cagttttcac tttatcgcca acgttgactt tcagttcctt cacgacgcct
    14701 gcaaacggag ccggaacttc catagacgct ttatcgcctt ctacggtgat cagtgactgt
    14761 tcagcggcaa ctttgtcgcc cactttcacc atcacttcgg tcacttcaac ttcgtcaccg
    14821 ccgatatccg gaacgttaac gtctttcacg ccagccgctg gtgcagaggc cgctgccgga
    14881 gccgcttcct gtttagccgc cggagctgcc gcgcctgctt cacccgcgac ttcgaagacc
    14941 ataatcagcg agccggtaga cactttgtca cccacgttca ctttgatctc tttcacggtg
    15001 ccagcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt gatcagcgac
    15061 tgttcagctt caactttatc gccaactttc accaggattt cggtcacttc aacttcgtcg
    15121 ctgccgatat ccggaacgtt aacgtctttt gccgctgcag ccgctggagc tgctgccgga
    15181 gcggcttctt tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc gtcggcggaa
    15241 tcgaaaatca taatcagtgc gccggtctgg gttttatcgc caacagagac tttgatctct
    15301 ttaacgatac ccgcctgcgg agacggaact tccatagagg ctttgtcgcc ttctacggtg
    15361 atcagcgact gttcggcttc aactttgtcg cccactttga ccaggatctc ggtgatttca
    15421 acttcatcag ccccgatgtc cggtactttg atttcgatag ccattattct tttacctctt
    15481 acgccagacg cgggttaact ttatctgcat cgatgttgaa tttggcgatt gcgtcagcaa
    15541 ccactttctt atcgatttcg ccacgtttag ccagttcgcc cagcgccgca accacgacat
    15601 aagaagcatc aacctcgaag tggtgacgca ggttctcacg gctgtcggaa cgaccgaagc
    15661 catcagtacc cagtacgcgg tagtcgtcag ccggtacgta agtacggact tgctcagcga
    15721 acagtttcat atagtcggta gatgccactg ccggagcgtc gttcatcacc tgagcgatat
    15781 acggaacgcg cggagtttcc agcgggtgca gcatgttcca gcgttcacaa tcctgaccat
    15841 cacgcgccag ttcggtgaag gaagtcacgc tataaacgtc agaacctacg ccgtaatctt
    15901 tcgccaggat ctctgctgct tcacggacgt gacgcaggat agaaccggag cccagcagct
    15961 gaactttacc tttgctacct tcaatggttt cgagtttgta gataccttta cggatacctt
    16021 cctcagcacc ttccggcatt gctggcatgt ggtagttttc gttcagcgta gtgatgtagt
    16081 agtaaacgtt ctcttgtttc tcaccgtaca tacgctccag accgtcatgc ataatgacag
    16141 caacttcgta agcgtaagcc gggtcgtaag agatacagtt cgggatagtc agcgactgaa
    16201 tgtggctgtg accatcttcg tgctgcagac cttcgccgtt cagggtggta cgaccggaag
    16261 taccgccgat caggaagcca cgcgcttgct ggtcaccagc cgcccagcac agatcgccga
    16321 tacgctggaa gccgaacatc gagtaataga tgtagaacgg aatcatcggc agattgttgg
    16381 tgctgtaaga ggtcgccgct gccagccagg aacacccagc gcccagctcg ttgatccctt
    16441 cctgcagaat ctgacctttc tcgtcttctt tatagtaagc aacctgctcg cggtcctgcg
    16501 gggtgtactg ctgaccgttc gggctgtaaa taccaatctg acggaacaga ccttccatac
    16561 cgaaagtacg cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc gacttgttct
    16621 tcagcattac gttcagggca cgaacgaaag cgatagtggt agagatctct ttgctctgct
    16681 cttccagcag cgcgccgaag tcttgcaggc tcggcagctc aagcttctcg gtgaagttcg
    16741 gctgacggct tggcagataa ccgtgcagtt tctgacgctg agcgtgcaga taggtatgct
    16801 cttcagaacc ttccgggaag gtgatgtacg gcaatttttc gatatcggca tcagacaccg
    16861 gcacattgaa acggtcgcgg atgtgacgca cgccgtccat gttcattttc ttaacctggt
    16921 gcgcgatgtt tttaccttca gccgcgtcgc ccatgccgta acctttaatg gtatgagcaa
    16981 ggattaccgt cgctttgcct ttggtttcct gcgctttctt aaatgcagcg tagattttct
    17041 tcggatcgtg accaccacgg ttcagtgccc agatctgttc gtcagtccag tctgcaacca
    17101 gtgctgcggt ttcaggatat ttaccgaaga agtgttcacg aacgtacgca ccatctttcg
    17161 atttgaaggt ctggtagtcg ccgtcaacgg tttcgttcat cagctggatc agtttaccgc
    17221 tggtatcttt acgcagcagt tcatcccaac ggctacccca catcactttg atcacgttcc
    17281 agccagcacc ttcgaagatg ccttccagtt cgttgatgat cttgccgtta ccggtgaccg
    17341 ggccgtcaag acgctgcagg ttacagttga taacgaaaac caggttatcc agtttttcac
    17401 gggtagcgat ggtgatcgca cctttggatt ccggttcgtc catttcaccg tcgccgagga
    17461 acgcgtaaac ggtttgttta gaggtatctt tcaggccacg gtgttccaga tatttcagga
    17521 atttagcctg gtaaatagca ccaatcggcc ccagacccat agatacggtc gggaactgcc
    17581 agaattccgg catcagtttc gggtgcggat aggaagagag gccattgccg tgaacttcct
    17641 gacggaagtt atccagctgc tcctgagtca gacgaccttc caggaaagca cgagcgtaaa
    17701 cgcccgggga gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc tcgttgcgtg
    17761 cacggaagaa gtggttaaag cacacatcat aaatggttgc ggaagactgg aaggacgcca
    17821 tgtggccgcc cagttcgagg tcttttttcg acgcacgcag aaccgtcatg atggcgttcc
    17881 agcggatagc tgaacgaata cggcgttcca gttccagatt acccggatac tccggttgtt
    17941 cttcaacggg gatggtgttg atgtagttgc tgataccggt gcctgcggct acgttgacac
    18001 cgcctttgcg ggcttcagca agcagttggt cgatcagata ctgagcacgc tcaacacctt
    18061 cttcacggat gaccgattcg atcgcctgga gccagtcgcg agtttcgatc ggatccacgt
    18121 catttgggaa acgttctgac atgggttatt ccttatctat ctaataacgt tgagttttct
    18181 ggaacctgtc ccattgaact ctcgccggaa agctcaataa gacaggttct acgtttagtt
    18241 gccgcgcttt tatatgcgct tgatttacaa catcttctgg ataattttta ccagaaaaat
    18301 cactaattct ttcgttgctc cagacgacgt agagaacgct cacggcggct ctcttcacgg
    18361 cttctgtcga gcaaaatttc ttcgataaag gccagatggc gatgcgatgc ttcgcgcgct
    18421 tcttccggct taccggccat aatcgcttca aatatgcggg tgcggtgact actcaccagc
    18481 ggcagcatct cgcgacgcga atagagcaat tcgaagttct ggcggacatt ctgggccaac
    18541 atcggctcca tacaccttag cagatgaagc agaaccacat tgtgggccgc ttcggtgacg
    18601 gcaatctgat actggagtac ggcatttgat tccgcgtcca gatcgccaga ctgctgcgcc
    18661 agctctatgg catggtggag ttcacggatg cgttccttgt cttcatcggt actacgcagc
    18721 gcggcgtaat aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa gtcatactgt
    18781 gattcaggat ggtcggagag cagctccacc agcggatcgc tgaagctttg ccataggctg
    18841 ctctggacaa aagtgccgcc accctggcga cgaagcaaca agcccttcgc ttcgagacgt
    18901 tgaatcgcct cacgcaagga gggacgggag acgtcgaact gttttgccag ttcgcgttcc
    18961 ggtgggagtt tttcgcccgg gcggagagtg ccttcgagga tcaaaaactc cagttgctgc
    19021 tcaatcacat cggagagttt tggttggcgg attttgctgt aggccatgag ttcctgtctt
    19081 aagccacttg ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc taaagtaaca
    19141 aagtattcac cttatgtcca tacaggtttt gattgaaatc atgaaactgt gcacatttta
    19201 acaacttgac atatataacg tttcaaagtt gtaactatgc acaaatgtta aatttaccca
    19261 tcaaggggtg agattaacag aattgaaacg aaaaaatatg tcctctgaac cgattcacca
    19321 agcaacaaaa cgcataacaa ttcaattatg tgaattaata tgtaaaatga atgttcaggg
    19381 aaggagagaa aggtagtatt tacctatgtt ttctttttac attgcttatt atcactgcaa
    19441 tcgccaccgt aaccttctcg tagcgaaaat catcacttaa ccagaacgtg atgattttca
    19501 ggtgacagat tctgtttaga attcattctg aatctttgaa caaagacaat ccttgcgaac
    19561 gccgccattc tttgacgatt ttcaggatgt tttcaggctt atcatctccc ttctttgcag
    19621 gatagtagat aagatcgctc atcaacggat gttgtgtaat tctggtaaga tgttcttcta
    19681 gatcatccca atatttgtca agctcttccc cttttaattg agcgttatcc ccggttggat
    19741 ttgtaaactc gccaagcaac tcaatgaatt cccgctctgt gtaatcttct actttatctt
    19801 taaaattata catttaacta tctcctgtaa taaatttcat catgcaaatg aggtgtgccc
    19861 tccttaaatt atcaatatcg taaatttcgc cactatattc aatcgcaaca caatgataca
    19921 t
//
LOCUS       DAZUCC010000078        19855 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZUCC010000078 DAZUCC010000000
VERSION     DAZUCC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.078"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..358
                     /gene="pduA"
                     /locus_tag="ACHY09_002429"
     CDS             74..358
                     /gene="pduA"
                     /locus_tag="ACHY09_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFS3885391.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            355..1167
                     /gene="pduB"
                     /locus_tag="ACHY09_002430"
     CDS             355..1167
                     /gene="pduB"
                     /locus_tag="ACHY09_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFS3885392.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1186..2850
                     /gene="pduC"
                     /locus_tag="ACHY09_002431"
     CDS             1186..2850
                     /gene="pduC"
                     /locus_tag="ACHY09_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFS3885393.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPANQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2860..3528
                     /locus_tag="ACHY09_002432"
     CDS             2860..3528
                     /locus_tag="ACHY09_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFS3885394.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKPVSFRAPVSATASSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3543..4061
                     /gene="pduE"
                     /locus_tag="ACHY09_002433"
     CDS             3543..4061
                     /gene="pduE"
                     /locus_tag="ACHY09_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFS3885395.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4071..5903
                     /locus_tag="ACHY09_002434"
     CDS             4071..5903
                     /locus_tag="ACHY09_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFS3885396.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSYVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     LGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKGAIHGK"
     gene            5893..6243
                     /locus_tag="ACHY09_002435"
     CDS             5893..6243
                     /locus_tag="ACHY09_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFS3885397.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            6263..6538
                     /gene="pduJ"
                     /locus_tag="ACHY09_002436"
     CDS             6263..6538
                     /gene="pduJ"
                     /locus_tag="ACHY09_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFS3885398.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6562..6984
                     /locus_tag="ACHY09_002437"
     CDS             6562..6984
                     /locus_tag="ACHY09_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFS3885399.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6987..7619
                     /locus_tag="ACHY09_002438"
     CDS             6987..7619
                     /locus_tag="ACHY09_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFS3885400.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKPLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVTVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVT"
     gene            7616..8107
                     /gene="pduM"
                     /locus_tag="ACHY09_002439"
     CDS             7616..8107
                     /gene="pduM"
                     /locus_tag="ACHY09_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFS3885401.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRAAIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHDRLLSYVDIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8111..8386
                     /locus_tag="ACHY09_002440"
     CDS             8111..8386
                     /locus_tag="ACHY09_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFS3885402.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRQVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8398..9405
                     /gene="pduO"
                     /locus_tag="ACHY09_002441"
     CDS             8398..9405
                     /gene="pduO"
                     /locus_tag="ACHY09_002441"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFS3885403.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHCFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9402..10787
                     /gene="pduP"
                     /locus_tag="ACHY09_002442"
     CDS             9402..10787
                     /gene="pduP"
                     /locus_tag="ACHY09_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFS3885404.1"
                     /translation="MNNSELETLIRNILREQLVPATQRAQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKNVSLKLITIIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKISVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VVSVAEYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10798..11910
                     /locus_tag="ACHY09_002443"
     CDS             10798..11910
                     /locus_tag="ACHY09_002443"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFS3885405.1"
                     /translation="MKTFSLQTRLYSGTGSLNILNRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIHFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLTNALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11907..13250
                     /locus_tag="ACHY09_002444"
     CDS             11907..13250
                     /locus_tag="ACHY09_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFS3885406.1"
                     /translation="MNVELTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVNVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGNLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLTVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLIPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13253..13807
                     /gene="pduT"
                     /locus_tag="ACHY09_002445"
     CDS             13253..13807
                     /gene="pduT"
                     /locus_tag="ACHY09_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFS3885407.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13807..14157
                     /gene="pduU"
                     /locus_tag="ACHY09_002446"
     CDS             13807..14157
                     /gene="pduU"
                     /locus_tag="ACHY09_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFS3885408.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14162..14605
                     /locus_tag="ACHY09_002447"
     CDS             14162..14605
                     /locus_tag="ACHY09_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFS3885409.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVNNNGVTELFALLHTEEGCR"
     gene            complement(14706..15035)
                     /locus_tag="ACHY09_002448"
     CDS             complement(14706..15035)
                     /locus_tag="ACHY09_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFS3885410.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15207..16265)
                     /locus_tag="ACHY09_002449"
     CDS             complement(15207..16265)
                     /locus_tag="ACHY09_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS3885411.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16463..16936)
                     /gene="sbmC"
                     /locus_tag="ACHY09_002450"
     CDS             complement(16463..16936)
                     /gene="sbmC"
                     /locus_tag="ACHY09_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFS3885412.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17055..18221)
                     /gene="dacD"
                     /locus_tag="ACHY09_002451"
     CDS             complement(17055..18221)
                     /gene="dacD"
                     /locus_tag="ACHY09_002451"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFS3885413.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVAMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18430..>19855
                     /gene="sbcB"
                     /locus_tag="ACHY09_002452"
     CDS             18430..>19855
                     /gene="sbcB"
                     /locus_tag="ACHY09_002452"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFS3885414.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSKFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
BASE COUNT         5071 a         5139 c         4993 g         4652 t
ORIGIN      
        1 gattaagtca gccattcttt ttcctcgctt ttttttatag tcatatcgtc aaaaaaatcc
       61 aggcgaggtc tttatgcaac aagaagcgtt aggaatggta gaaaccaaag gcttaactgc
      121 cgccatagag gccgcagacg ccatggtgaa gtcggcaaat gttttgctgg tcggctacga
      181 aaaaataggt tccgggctgg tgaccgtcat cgttcgtggt gatgtcgggg cggtaaaagc
      241 ggcaacagac gcgggtgccg cagctgcgcg taacgtcggc gaagtaaaag ccgtacacgt
      301 tattccccgc ccacataccg atgtcgaaaa aatcttacca aagggaatta acccatgagc
      361 agcaatgaac tggtcgaaca gatcatggcg caggtcatcg ctcgcgtagc gacgccagag
      421 catgaggttc tcccgaaaac cactcatcaa atacgagaga cggctatggc agaaaaaagc
      481 tgcacgttaa cagaatttgt tggcaccgct attggcgaca ctgtaggtct ggtcattgcg
      541 aacgttgata gcgccctgtt ggacgcgatg aaacttgaaa aacgctatcg ttccattggc
      601 attctggggg cgcgtattgg cgcagggcca cacattatgg ccgctgatga agcggtaaaa
      661 gccaccaaca ctgaagttat tagcattgaa cttcctcggg atactaaagg cggtgccgga
      721 cacggctcat tgatcctctt aggtggcaac gatgtttccg acgtgaaacg cggtattgaa
      781 gttgccctga aagaactcga tcggaccttt ggtgatgtct atgccaacga agccgggcat
      841 cttgaactgc aatacaccgc ccgcgccagc tatgcactgg aaaaagcgtt tggcgctcca
      901 ctgggacggg cttgcggcgt tattgtcggc gctccggctg cggtgggtgt tctgatggcc
      961 gacaccgcgc tgaagtccgc caatgtggat gtcgtggcat acagctcccc cgctcaaggc
     1021 accagctaca gcaacgaagt cattctgatt atctcgggtg actccggcgc agtccgccag
     1081 gcggtcatct ccgcacgcga aatcggtaaa accgtgcttt cgactctcgg cgatacaccg
     1141 aaaaacgatc gcccttccta catctgaatt ctctgaggca gatttatgag atcgaaaaga
     1201 tttgaagcac tggcaaaacg cccggtcaac caggacggtt tcgttaaaga gtggattgaa
     1261 gaaggcttca ttgcgatgga aagcccgaac gatcctaaac cgtcaatcaa aattgttaac
     1321 ggcgtcgtta ccgaactgga tggaaaaccc gccaatcagt ttgacctgat cgaccacttt
     1381 atcgcccgct acggcatcaa tctcgcgcgc gcagaagaag tcatcgcgat ggattcggta
     1441 aaacttgcca atatgttgtg cgatccaaat gtcaaacgta gcgatatcgt tccccttacc
     1501 acagcaatga cgcctgcaaa aatcgtcgaa gtggtttcgc agatgaacgt ggtagagatg
     1561 atgatggcga tgcagaaaat gcgcgcccgt cgaacaccat cacaacaggc tcacgtcacc
     1621 aacatcaaag ataacccggt acaaattgcc gccgacgcag ctgaaggcgc atggcgcggg
     1681 tttgatgaac aggaaaccac cgttgccgtt gctcgctatg caccgttcaa cgccatcgcc
     1741 ctgctggtcg gctcacaggt gggacgtccg ggcgtcttga cccagtgttc actggaagaa
     1801 gccaccgaac tgaaactcgg tatgttaggc cacacctgtt acgccgaaac aatttctgtc
     1861 tatggtacag aacctgtttt caccgatggc gatgataccc cgtggtcaaa agggtttctc
     1921 gcgtcatcct atgcgtctcg cggcttaaaa atgcgcttta cttccggctc cggctctgaa
     1981 gtacagatgg gttacgcgga aggcaaatca atgctttatc tcgaggctcg atgcatttac
     2041 atcaccaaag ccgcaggcgt tcagggtctg cagaatggtt cggtaagctg tatcggtgtt
     2101 ccatcagctg tgccatcagg catccgcgca gtattagcgg aaaacttaat ttgctcatcg
     2161 ctggatctgg aatgcgcctc cagtaatgac cagaccttta cccactcgga tatgcgccgt
     2221 accgcacgtt tcctgatgca atttctgcca ggtaccgact ttatctcttc cggtttttcc
     2281 gctgtgccga actacgacaa catgtttgct ggttcaaacg aagatgctga agacttcgat
     2341 gattacaacg tcattcagcg tgacctgaag gttgatggcg gcctgcgacc agtacgcgaa
     2401 gaagatgtta tcgctatccg caataaagcc gctcgcgctt tacaggctgt ctttgccggt
     2461 atgggactac cgcctattac cgatgaagaa gtcgaagcgg caacctatgc ccacggttca
     2521 aaagatatgc ctgagcgcaa tattgtagaa gacatcaagt tcgcccagga gattatcaat
     2581 aaaaaccgga acagcctgga agtggtgaaa gccctggcgc aaggcggttt taccgatgtc
     2641 gcccaggaca tgctcaacat gcaaaaagca aaactgaccg gcgattatct ccatacctca
     2701 gccatcatcg tcgatgacgg acaagtgctc tctgcggtca atgacgtcaa tgattacgcc
     2761 ggaccggcta caggttaccg cctgcaaggg gaacgctggg aagagatcaa aaatattccc
     2821 ggtgcacttg atcccaacga aattgactaa gggtgagaaa tggaaattaa cgaaaaatta
     2881 ctgcgtcaga ttatcgaaga cgtgctggca gaaatgcaaa ccagcgacaa gcccgtttca
     2941 tttcgcgcac ctgtctctgc aactgcctct tccgcgccag ataccggaaa ttttctgacc
     3001 gaaattggcg aagcccaaca aggcactcag caagatgaag tcattattgc tgtaggcccg
     3061 gcgttcggcc tggcgcaaac cgtcaatatc atcggtattc cgcataaaaa cattttgcgc
     3121 gaagtgattg ccggtatcga agaagaaggc attaaagccc gtgtgattcg ctgctttaaa
     3181 tcgtccgatg tcgcattcgt agcagtcgaa ggcgatcgtc ttagtggttc aggtattgct
     3241 atcggtattc agtcgaaagg cacaacggtt atccaccagc agggacttcc tcccttgtct
     3301 aacctggaac ttttcccaca agcgcctttg ttgacgctgg atacttatcg tcagattggc
     3361 aaaaacgccg cgcgttatgc aaagcgtgag tctccacaac cggtgccgac cctcaacgat
     3421 caaatggcgc ggccgaaata ccaggccaaa tctgccattt tgcatattaa agagacgaag
     3481 tacgtggtca ccggcaaaaa accgcaagaa ctgcgcgtga cgttttaaca aaggaaacct
     3541 tcatgaatac cgatgcaatt gagtcgatgg tcagagacgt gttgaaccgc atgaacagtt
     3601 tacaggatgc agctcctgtt tctgcggtcc ccaacgcgtc aatacagagc gccaaagtga
     3661 cggattaccc gctggcaaat aaacatccgg aatgggttaa aacggcgacc aataaaacgc
     3721 tcgacgattt cactctggaa aatgtgctaa gcgataacgt caccgcccag gatatgcgta
     3781 taacccctga aacattgcgt attcaggccg caattgccag agatgctggt cgcgatcgcc
     3841 tggcgatgaa ttttgaacgc gccgctgagc ttacctcagt accggatgat cgcatcctcg
     3901 aaatctacaa tgcgttacgc ccttatcgtt cgactaagca agagcttatt gccattgctg
     3961 acgatcttga acggcgttat caggccaaaa tttgtgctgc atttgtccgt gaagctgcgg
     4021 aactttatgt cgagcgcaaa aaactgaaag gcgacgatta aggacgaact atgcgatata
     4081 tagcaggcat tgatatcggc aactcatcaa cagaggttgc gctggcaacg ctcagtgcaa
     4141 ctggagagtt gtcgtacgtg agtagcgccc tggcggaaac caccggaatt aaaggtacgt
     4201 tgcgtaacgt ccatggtatt caggaggcgc tcgcacaggc aacgaaaaaa gtcggcatca
     4261 atgttagcga tatttcgctt attcgtatca acgaagcaac tccggtcatt ggtgatgtgg
     4321 cgatggaaac catcactgaa accatcatca ccgaatcaac catgatcggt cacaacccga
     4381 aaactccggg cggagttggg ctgggcgtag ggctcaccat cactccgcaa gagttattaa
     4441 cacgcccggc ggacacacct tacatcctgg tggtgtcatc ggcatttgat tttgccgata
     4501 tcgcaacgat gataaacgcc tccgtacgag ccggatatca gctgactggc gttattctgc
     4561 aacgcgacga tggcgtactg gtcaataacc gcctggaaat tcctctccct attgtggatg
     4621 aagtcctcta catcgatcgt attccgctcg gcatgctggc tgcaattgaa gttgccgttc
     4681 ccgggaaagt tatcgaaacc ctgtctaacc cgtatgggat tgccaccgtt ttcgccctca
     4741 atgctgaaga gactaaaaat attgttcctg tcgcgcgggc gctaatcggc aaccgttcag
     4801 cagtggtagt gaaaacgcct tctggcgacg ttaaagcccg ttcgataccc gccggaaata
     4861 tcgaactgct ttctgccggg cgcacaacgc gggttgatgt agctgctggt gccgatgcca
     4921 ttatgaaagc ggtcggtgag tgcccaaaac tggaaaacgt gacgggagaa cctggctcca
     4981 acatcggcgg aatgctggaa catgttcgcc agaccatggc tgaactgacc aacaaaccca
     5041 gcaacgaaat ttttattcag gatttgctgg cgatagatac ctctgtcccc gtcaacgtaa
     5101 ccggtggtct ggcaggtgaa ttttcactgg aacaggctgt gggtattgcc tctatggtga
     5161 aatccgaccg tctgcaaatg gcgatgatcg ccagtgaaat taagcaaaaa ctgcatgttg
     5221 atgttcaggt cggaggcgca gaagcagaag ccgccattca gggggccttg actacccctg
     5281 gaaccacacg tccactggca attctggact taggtgcagg ctctaccgat gcctccatca
     5341 tcaatcagaa aggggaaata gttgccactc accttgcggg agcgggcgac atggtaacga
     5401 tgattatcgc tcgtgagctg ggcttaaatg atcgttatct cgcagaagaa atcaaaaagt
     5461 acccgttggc aaaagtagaa agcctgttcc acttacgcca tgaagatggc agcgttcagt
     5521 tcttccctac tccgctgtcc cctcatgtat ttgcccgcgt ttgtgtggtg aaacctgacg
     5581 aactggtgcc tattctcggt gatttgacgc tggaaaaagt acgtgctgtc cgtcgtagtg
     5641 cgaaagagcg tgtcttcgta actaatgccc tgcgcgcgct acgtcaggta agccctgccg
     5701 gaaatattcg cgacattccg ttcgtcgttc tggtgggtgg ttcttcgctg gatttcgaag
     5761 taccgcaatt ggtcaccgac gcgctggcac attatcgact ggtcgccggg cgggggaata
     5821 tccgtggtag cgaaggtccg cgcaatgcgg tagctactgg gctcctcatc gcctggcaca
     5881 agggggcgat tcatggaaag taaagagcgt gcaccagcca tcgttgtcat ggagatcggt
     5941 gactgtatca ctacatggga tgaagtgctt cttggcattg aagaggaagg tattcccttt
     6001 cgcattcaac acattccgtc aggtgaagtt atcgatagcg cctggctggc ggctcgtcaa
     6061 tctcccctgt tggttggcat tgcctgcgat caggaaaaac tgattgtgca ttacaaaaac
     6121 ttaccggcat cagcgccact gtttacgctg atgtatcaac aagataacca tgccaggcgc
     6181 agcatcggca ctaacgctgc ccgcctggta aaagggattc ccttccggga actccactca
     6241 taatccacag gagaaacgca gtatgaacaa cgcactagga ctggttgaaa caaaagggtt
     6301 agttggcgca attgaagctg cagatgccat ggttaaatcc gccaacgtgc agctggttgg
     6361 ctatgaaaag attggttcgg gtctgattac ggtgatggtc cgtggcgatg ttggcgcagt
     6421 aaaagccgcc gttgatgcag gaagcgccgc cgcaagcgcc gttggcgaag tgaaatcctg
     6481 ccatgtcatc ccgcgcccac acagtgatgt tgaggctatt ttacctaaat cagcataacc
     6541 ccattcgcta aggagcacag cgtgaagcaa tcactgggat tacttgaagt cagtggtctg
     6601 gcactcgcca tcagttgcgc agatgctatg gcaaaagccg cgtccatcac gctggttggc
     6661 cttgagaaga ccaatggctc aggctggacg ctcatcaaaa tcaccggtga cgtcgcctcg
     6721 gttaatgcgg cgatcgcgac gggagccagt ttcgccgggc agcgaaatgg ccttgtcgca
     6781 cacaaagtaa ttgcccgccc gggagacggc attctgcccc acacaactga agcattgact
     6841 gtgacggttg aaacaagcga aacaccagca acaaccgtca tctcagagat tatcagttgt
     6901 aatttgtgtc ttgacccggc atgtcagcgg caaaaaggtg agccccgctc actttgcctg
     6961 tacgccaata agcgagggga gtagcagtgg ataaacaaac cctgcaggaa acagtgagcc
     7021 agattctgga tgagatgcgc gagcgcccta tcccactggg cgtttccagt cgccatattc
     7081 atctttcaac agcggattat ttgcgtcttt ttcctgaaca accgattagc gagaaaaagc
     7141 cgttaatgca accgggccaa ttcgctgctg aacaggttgt tacgctggag ggaccaaaag
     7201 gcaaattgca caacgttcgc ttgttggggc cgctacgcaa tgcaagccag gtggaaattt
     7261 cccggacgga cgccagaacg ttagggatca ccgccccgct acgcatgtca ggcgatctca
     7321 gcggcactcc ggggattcgt ttgataagcc catttgcgga actggagatc acatctggcg
     7381 tcattgtcgc gcaacggcat attcatatgt ctccgctgga tgccctgatt tatggtgtta
     7441 cgcacggcga taaagtggcg gttgccattg aaggtgatga gcgacgactg atattcgaaa
     7501 acgtcactgt tcgcgtctct ccggatatgc gtcttgaaat gcatattgat accgatgaag
     7561 ccaacgccgc aggggctgat agccctgacg tttttgccac actggtgtca gtgacatgaa
     7621 taacatgctg atgcaacgta ttgttgacga agtggttttt cgccttaaac aacgtgcggg
     7681 aaaaacactc gtgctgactg tttttcaact gcgagacgcc agcgttcaag agagcgttca
     7741 tcagtatgcg tcattacaaa tccgatatgt cgatctgccg ctgctgcgac aacttgcgga
     7801 gaacgaaacg tcagatcgag cagcaatcca gatacacgaa gcgttagcct ggggaatgca
     7861 cattcaattg tcgttgcagc gtcatttcct gaacgccatt gaattgaaga ctctcgcccg
     7921 tctaccgcta agctggtgcg acgaacaagg gcaaccgatc tacctacatc atgatcgact
     7981 tttaagttat gtcgatatcg cacagttaag ctcgggaata ttggtgttac agcggaaatg
     8041 ttgtgtgacc gcactggctc gtgaagcggc aattacccga aatattcagt taatcaggca
     8101 ggagtaacct atgcatctgg ctcgagtcac tggcgcggta gtatccacgc aaaaatcacc
     8161 ttctctgatc gggaaaaaac tgctgctggt tcgccaggtc agtgctgacg gcgaactgcc
     8221 tctcacgcca ttaaccggcg acgaagttgc cgttgattcc gtcggcgcag gcgttggtga
     8281 gttggtactc ctcagcagtg gttccagcgc cagacatgtg ttttccggcc ctaacgaagc
     8341 catcgacctg gcagtggtcg gcattgtcga tacgctttcg cgctaaggac ctgaaagatg
     8401 gcaatttata cccgaacagg tgattcagga agtacgtctt tatttaccgg acaacgcgtc
     8461 agcaaaactc atctacgtgt cgaaacctat ggcacgctgg atgaactgaa cgcgacactc
     8521 agtctgtgtt attgcgccac cgccatcgaa agccatcgca tcttactgga agccattcaa
     8581 caacagatat tttggtttag cgccgaactc gccagtgaaa gcgaacagcc gtcagcgcag
     8641 cagcgttaca tcggtacgga agaaattgcc gcgctggaaa acgcgatcga tagcgcaatg
     8701 aacgccgtcc ccccggttca ctgcttcatt ctgcctggtc gatgtgaagc cgcaagccgc
     8761 atgcatttcg cccgcacggt ggctcgacgg gctgaaagac gtctggttga actgacaaca
     8821 gaaaccaccg tccggaatgt tttgctgcac tatatcaacc gactttccga ttgcctgtat
     8881 gccctcgccc gcgtggaaga caacgttgct catcaaaacc tgatgattca ggaaatcaca
     8941 aaacgttatc acgaagccaa ccacacacca gcattgaagg aacgcacaat gccgctcact
     9001 tttcaggatc ttcaccagct cattcgtagt gcggcaatgc gcgcagatga actgcatatt
     9061 cccgttgtca tcagcattgt cgatgccaac ggaacagaaa gtgttacctg gcggatgcct
     9121 gatgccctgt tggtcagcag tgaactggca ccgaaaaaag cctggaccgc cgtggcgatg
     9181 aaaacggcaa cccacaagct tgctgatacc gtgcaacccg gcgcgccgct ttatgggctc
     9241 gaaagccata tgcaaggcaa agtcgtcacc tttggtggcg gcttccccct ctggcgtgac
     9301 ggaaaattgc ttggcgggct tggcatcagc ggcggtagcg ttgaacaaga catggatatt
     9361 gcccaaagcg caatggcggc aattaacgtg ggagtaaacc aatgaataac tctgaattag
     9421 aaacactgat ccgcaatatt ctgcgtgagc agcttgtccc cgctacgcaa cgagcgcaaa
     9481 gaaacgctat tttccagact gttgatgaag cggtctgcgc agcacaccag gcctttttac
     9541 gctttcaaca gtgcccgctt aaaacccgta gcgccattat cagcgccatt cgcgaagaat
     9601 taatgccccg ccttaccgaa ctggcagaag aaagcgccaa agaaacgggg atgggaaata
     9661 aagaagataa gtacctaaaa aacaaagcgg cacttgataa cacgcctgga attgaagatt
     9721 taaccaccac agcactgacc ggtgatggag gcatggtttt gtttgaatac tctccgtttg
     9781 gggtgattgg ctcggtcacg ccaagcacca accccacgga aaccatcatt aacaacagca
     9841 tcagcatgct ggcagcaggt aatagcgtct acttcagtcc acacccggga gccaaaaacg
     9901 tttcgttaaa actgattacc ataatcgaag acatcgcgtt tcgctgtagt ggcattcaca
     9961 acctgatcgt taccgtatct gaaccaacct ttgccgcaac cgaacaaatg atggcgcacc
    10021 ctaaaatcag cgttctggcg attaccggtg gcccggggat cgtcgccatg ggaatgaaaa
    10081 gtggcaagaa agtgattggt gccggagcgg gtaacccacc ctgcattgtc gatgaaacgg
    10141 cggatattgt gaaagccgcc gaggacatca tcaacggcgc ttcatttgat tacaacctgc
    10201 cgtgtatcgc tgaaaaaagc ctgattgtcg tggtatcagt ggcagagtat ttaattcagc
    10261 agatgcaaag ctttggagca cttcttctta accatgaaca aacggagaaa ttgcgtgcaa
    10321 tttgcctgcc agacggtagc gccaacaaaa aactggttgg gaaaagccca tcagccttgc
    10381 tggaagcagc gggcctccct ttgcccgcaa aagcgccgcg cctactgatt gctgttgttg
    10441 acgccgatga tagttgggtt acgtgtgaac aactgatgcc aatgctgccc attgtgaagg
    10501 tcaacgattt tgacagcgct ctcgccctgg cattgaaagt ggaagacggc ttgcaccaca
    10561 cggcaattat gcactcgcag aacgtctcac ggctcaacct tgccgcgcgc gttatgcaaa
    10621 cctcaatctt tgtcaaaaac ggcccctcct gggcgggtat tggcgtcggc ggcgaaggct
    10681 ttacgacctt taccattgca acaccaaccg gagagggtac aacgtcagca cggacttttg
    10741 cccgctcacg acgctgcgtg ttaaccagcg gcttttccat tcgctagaga aacggaaatg
    10801 aaaacttttt cactgcagac gcgtttatac agcggaacgg gaagtctcaa catactgaac
    10861 cgtttcacga ataggcacat ctggattatt tgcgacgtct ttctggcaca ctcaccactc
    10921 attgacacgt tgcatcaggc gctgcccgac agtaatcgtc tcagtatttt tagcgaaata
    10981 acgcccgacc caactatcca gaccgtggtg aaaggcattg cgcaaatgca gactttacgc
    11041 ccggatgtgg ttatcggttt tggcggcggt tctgccctgg acgccgcaaa agcgattgtc
    11101 tggttcggtc gccagtgtgg cattgaaatt gaaacctgcg tagccattcc taccaccagc
    11161 ggtaccggtt ccgaagtgac cagtgcttgt gtcatcagcg atcctgagaa agggattaaa
    11221 tatccccttt tcgataatgc actttatccg gatatcgcga tccttgatcc atctttggtc
    11281 gtcagcgtgc caccggcgat aacggccaat accgggatgg atgttttaac ccatgcgctt
    11341 gaagcttatg tttctccccg cgccagcgac ttcacggatg cactggtcga aaaagccgtg
    11401 caaattgttt ttcaatacct gcccactgca gtgaagaaag gcgattgtct tgccacccgt
    11461 ggcaaaatgc ataatgcatc gacgcttgca ggaatagcct ttagccaggc cggactcggt
    11521 ttaaaccatg ccattgctca tcaactcggc ggacagtttc atctgccgca cggcctggcg
    11581 aatgcgctcc tgctcacggc ggttatccac tttaatgctg gcgatccgcg cgcagccaaa
    11641 cgctacgccc ggctggcgaa aacgtgccat ttatgcccgg acaacgccaa tgacaccgcc
    11701 agcctgaatg cacttattca acatattgag cagcttaaaa caacctgtac gctgccgact
    11761 cttaccaatg cgctgaaaga aaaaaaagca gaatggtcaa tacgcatacc agatatggtt
    11821 caggcggcac tggcggatgc aacgttgcgt accaacccgc gtgcggctga tgcctccgct
    11881 attgcagaac tgctcgagga gttgttatga acgtcgagtt aacctcctgc gacgaccaga
    11941 ccatccgcga gcgagtacag gcagcaggtg tcgtgggtgc tggcggcgca ggttttccaa
    12001 cccatattaa gctacaagcg cgtgttgaca cttttctggt gaatgcggcg gaatgcgaac
    12061 caatgttgaa agtggatcaa cagttaatgg tgcagcaagc cagccgcctg cttcgcggtg
    12121 ttcactatgc gatgaaagcc accggggcca gtagcggaat catcgcgctg aaagagaaat
    12181 atcaacgggc aataaacgca ctcacccccc tattaccacc ggatatccgc ctgcatattt
    12241 tgcccgacgt ttaccccgca ggagacgaag tgctgaccat ctggatggca actggtcgcc
    12301 gcgttccacc cgctgcatta cccgttaacg tcggtgtggt ggtcaataat gttcaaaccg
    12361 tacttaatat tgccagagcc gtcgagcaac agtatccagt gacgcgccgc acactcacta
    12421 ttaatggtgc agtggcgaaa ccaataacgg taaccgtccc catcggcatg tctttacgtg
    12481 aagtcctggc gctggcgggc ggcgcaacag tagatgctcc tggttttatc aatggcgggc
    12541 cgatgatggg aaatttgatc acatcgctcg acacccccgt cagcaaaacc actggcggct
    12601 tgctggtttt acccaactct cattcactga tccaacgtcg aatgcagaat gaccgcagcg
    12661 tacttaccgt cgcgaaaact gtttgtgagc aatgccgctt gtgtaccgat ctctgcccac
    12721 gtcatttagt cggtcacgaa ctggcacccc atttattagt acgtgcggtt aactaccaac
    12781 agctcgctac gccacaactg ttactgacgg cgctcacctg ttcggagtgc aacgtctgcg
    12841 ccagcgtcgc ctgtccggta ggaatttcac caatgcgcat aaaccgcctg cttaagcagg
    12901 aattacgcgc acaaaatctc cgctacgaag gtgccttaaa tcctgctgat cccatggcaa
    12961 attatcgact gattcccgta aaacggctgg taaccagact gggcctaatc ccgtggtatc
    13021 aggatgcgcc attgtctgaa caggtgcctc agcccgaaaa agtgacgtta ttgttacgcc
    13081 agcacatcgg tgccagcgca attgcgtgtg tacaaaaagg cgatcgcgtc gttcatgggc
    13141 aatgtgtagg tcaaattcca cacggcacat taggtgcccc cattcatgcc agtattgatg
    13201 gcatggtcag cgatgtgaca gaaaacgcga ttacgcttgt gagaggttaa cgatgtcgaa
    13261 agcggtaggt attttagaac ttagcagtat cgcgaaaggg atggaaaccg gcgacgccat
    13321 gctgaaaagc gcaaacgttg agttactggt cagcaaaacg ctatgccccg gaaaatttct
    13381 gctgatgctc ggtggcgatg tgggtgcggt gcaacaggcc atcaacagcg gtgcttccct
    13441 tgctggtgaa atgctggtcg acagtcttgt cctgccaaat attcatccca gtctcctttc
    13501 tgcaatcagc ggactgaacc atgttgaaca gcaccgggct gtcggcgtag tcgaaacctg
    13561 gagtgtcgcc gcctgcattg acgctgccga ttgtgctgtc aaagctgcga atgtcattct
    13621 cgttcgcgta catatggctt ttggcattgg tggtaaatgc tacatggtgg ttgctggcga
    13681 tatttcagat gttaataacg cggtctctgt cgccagcgag cgtgcggggg aaaaaggttt
    13741 gttggtctat cggtcagtga ttgcccgtcc acatgaagcc atgtggcgtc agatggtgga
    13801 gggataatga caactcagca cacaactgaa cggatgattc aggaatatgt ccctggtaag
    13861 caggtcacgc tggcacatgt catcgccaat cctgggaagg atctgtttaa gaaattgggt
    13921 ttaccagaca ccgtttctgc tattggcatt ctgacgatta cccccagcga agcatctatc
    13981 atcgcatgtg atatcgccac aaaatcaggc gctgtagaga tcggttttct tgatcgtttt
    14041 accggtgccg tagtgctgac tggtgatgtt tctgctattg aatatgcact tagccaggtt
    14101 acccgtacgc tgggtgaaat gatgcgcttt accgcctgcc ccattacgcg gacctgacgt
    14161 tatgcaacgt atgatgctta tcggccccag ccagtgcgga aaaacgtcac tcacgcaatg
    14221 tctgcgtggc gaaaatatcc attatcagaa aacgcaggca atcgtctggt ccccgaccac
    14281 cattgataca ccaggcgaat acctcgaaaa ccgcagcctc tacagtgcat tgttggtcag
    14341 tgcctgcgaa gcagacatta tcgcgctggt acttaatgcg gacgcaccgt ggtcaccttt
    14401 ttcgccaggc tttacctcgt caatgaatcg cccggtcatt ggagtagtaa ccaaagcaga
    14461 tttagccagc atggaacaaa tatctttggt gaaatgttgg ttacgggaag ctggagcgca
    14521 caacgtgtta gtaaccagtg cggttaataa caatggagtt acagaacttt ttgccttgct
    14581 gcatacagaa gaaggctgcc gctaatttcg ataggttctc agatgagaat gatgacggtg
    14641 atacacagat aagaatcaat gagcaatgag ggttgccggg caaccctcat tgaataaaac
    14701 aggaattact tcgcttcgcc gtttttcatt tcgcccatag ctttcagctt tttggagata
    14761 tcgcggcgtt ctttggagag ctcggcattt ttgatgatgt aatcgtcaac gcgatcttca
    14821 tagtcacctt tcatgctggc gatgatgccc tggattgctt caacgctcat ccctggcttg
    14881 atgtaatcgc tcaggttgtc cagcagcagg acgcgcttct ggttgtcacg gatctttttc
    14941 tcaacgtcct gaatttcacg ttgcagtttg ttcttacgac ggaacagacg aacaaattcc
    15001 agtacgtcct ggaatgaagg cttggtagtt tccattttta tacccctgat aatgtgagag
    15061 tcggattcgt ttaagcaacc gctattcgtt agggccaaca ttactcatag ctgccgcaga
    15121 tgacaatgct tttatccctt ttcactatca taccctttat ccttgctgaa tcgaagcagc
    15181 agcaagatga ttctgaagtt caggaattat ttgcgcaagg cccggcaaat cagattcgat
    15241 agcaactcaa gctgatgtgc cgtttccatg ttcagccaca cataaccata gattggtgtt
    15301 tccacaacct tcaggtcatg gtggttatcg agcaactgac gcagctcttc cacagcatcg
    15361 ttcaattttt ccgtattggc aaaaaccggc tgcggattac cttcgtacag cgcatgaaca
    15421 aggctcagca gtatttgctg catcatgtgc tgggtatcac gaagtttttg cgcgttcaat
    15481 aacacgaaat ggctggggcg cgtggcccag tatgcattga tttgcaactc cagcatacaa
    15541 accagattgc gattaatggt ctggatacct tcatatatcg attttggaat acgggtttct
    15601 ttgctggcgg gcgcaatcag cccacgcatt ttcacggcat cggtcaggag tttttgtaga
    15661 tggctttcca gacgtgggcg ttcgagtaag ttcggtgaga atgcagattg atagacccga
    15721 ttatactcgg tcagactttt cgccagttga atgcgccagt ggatgaacgc ccgttgtggc
    15781 cagataccgg taaacaacat tgccagtaaa gagccgagga tcacatcgcc gcttcgccat
    15841 aacgccgtat caatttcacc tgtcggggaa cccacaacaa ttgccagcgt caccccaatc
    15901 aataaacctt gatatggttt cttgcccagc gccagccaac cgcaaaggaa catggccgcc
    15961 gcgcaccaga ctaacatcag cggtaacgag attaactcca gttgcagagc gataagacct
    16021 aaaatcgacc ccaacaccgt accgccaata cgttcgaagg cacgagggac aacgttcccc
    16081 cagaacgaga ttggccccat aatcaccacc atggtgacca gcggccaggt gctttccggg
    16141 atagtaaaca ggcggataat gagaaaagtg agcaggaatg ccagcgcgac ccgagtacca
    16201 tgcacaatgc ggtaatggcg gtatacccgg atttcaaacg ggcttaatga cttatcggca
    16261 cgcacgcaga aactccgtaa tgaagattaa aagcgctcat aactcatctg tcggccagaa
    16321 ggcgcgcctc tgaattatcc gacggacaaa ccgggaacag tgtaatttcc cggcaggagc
    16381 gaaaaaattg aaaggcgcat cttactcttt tcgctttcaa aaaaagagtg gtcttcgcgt
    16441 caacacaccg ccctgagatg aattagtgat gttttggctg caccgcaaca tacatttcga
    16501 tatcccagta cccatcttcc gcgccattgt tcaagtaaac ctcgaagcag ggctttggta
    16561 acatttcata agcactgtcc tgcaagaggc tattaaagaa ctgataccag ggtttagcaa
    16621 aatcatcacc gactacacga gccaccgcca ccgcatactg accacctgaa atttctgtca
    16681 gaatgacgcc ctcactgttt tcggggagcg taaagttatt cggtaccgtc acgacggtgt
    16741 cgcagcgtaa tttttcggcg ggtgtttcat ctggattgtc gtaatagaca gcaacccact
    16801 ccttcggcac aatatttttg ctatctatcc acatcatcaa ctgctcaaaa cctttcttta
    16861 ccgtctgctc ccacgggcca acaagatgga aacctgcaac ggtacgtttc tcttcctgct
    16921 taatctcgta gttcatgacg cctccattaa cactgttttt atatacagta tagttgcaat
    16981 attaaaacca caaggaatga gtgttgatta tgcgagcaga ctcgcactcc tgccagtctg
    17041 ctgcaaaaga aaggtcaggc cttatggtgg aaataatcac tcaggcggga aaacatgccg
    17101 ccttccccga cagattccag ggtaaccagc ggccagtgcg ccacctgttt atcacggtcg
    17161 taaagttcaa tttcccctac ccgctgatgg gcgctaattg gcgcggtgag ctctttacca
    17221 tcaagggtat atttggcttt gatatgtgga atttcggctt tcggtagcac catccagaac
    17281 tcttgttccg ttcccaggtc gatattttct ttatcgccat accagatgcg ttccgttccg
    17341 acctttttcc cacggtgcaa aatttgcacc gtagtaaagt tttgttgccc ccaacgcagt
    17401 aattttcttg cctcttcctc acgacctttt gcactgtcag cccccattac cactgcaatg
    17461 agacgacgct gcccatctac agccgaagca atgagattaa acccggcacc agaagtatga
    17521 cccgttttca ggccgtcaac attcatggtt ttatcccaca acaacccgtt acggttttgc
    17581 tgggtgatac cgttccaggt gaggcttttc tcactgtaca tatgataaaa ctcgggctcg
    17641 ccgtggatga tagcgcgaga aagcacagct aaatcataag ccgagctatg ctggccaggt
    17701 gcatccagac catgcactgt ttcaaaatgc gtatccttga gatgcagctt ctcggcatag
    17761 ttgttcatca ttgcaacaaa ctgccgttgc ccaccggcaa tatagtcagc cagtgcaaca
    17821 caagcatcat ttccggaatc cacaattaaa ccacggctta aatcacgtac cgatacgcga
    17881 tcgccctctt tcaaaaacat cagtgaagaa ccgacaaaca ctggattatc tttcgcccac
    17941 gcatcgcgcc ctacggtgac aatatcgtct ggcgtaattc gatgactatc gatagcgcga
    18001 tccacgacat aacccgtcat cagctttgtc aggctggcgg gattgcgctg ttgatgctca
    18061 ttacccgcgg tgaggatctg tccggtggtg taatccatca atacccagga cccggcatga
    18121 atctctggag gctgaggtga aaaaggaatg ttttccgccg caaaaccaga cgataagtta
    18181 aaaacgaaca aagaagcagc aataataaga cggcgtttca acagcaaacc ctcaggagtt
    18241 tcaaatagct gttcttttta cggaaatact tatgaactgg ctggaataaa gtgcaagaaa
    18301 atgtgactaa cctctcattt ttatctgaca tgatctgttg ccactcgctg ccaaattgtg
    18361 gcgctaaagc tgattagcac ggtgatattt gatactctgg cagacagcag agataacgga
    18421 tttaacctaa tgatgaatga cggtaagcaa caatctacct ttttgtttca cgattacgaa
    18481 acctttggca cgcaccccgc gttagatcgc cctgcacagt tcgcagccat tcgcaccgat
    18541 agcaaattca atgtcatcgg cgaacccgaa gtcttttact gcaagcccgc ggatgactat
    18601 ttaccccagc ctggagcagt attaattacc ggtattaccc cgcaggaggc acgggcgaaa
    18661 ggagaaaacg aagccgcgtt tgccgcccgt attcactcgc tttttaccgt accgaagacc
    18721 tgtattctgg gctacaacaa tgtgcgtttc gacgacgaag tcacacgcaa cgttttttat
    18781 cgtaatttct acgatcctta cgcctggagc tggcagcatg ataactcgcg ctgggattta
    18841 ctggatgtta tgcgtgcctg ttatgccctg cgcccggaag gaataaactg gcctgaaaat
    18901 gatgacggtc taccgagctt tcgccttgag catttaacca aagcgaatgg tattgaacat
    18961 agcaacgccc acgatgcgat ggctgatgtg tacgccacta ttgcgatggc gaaactggta
    19021 aaaacgcgtc agccacgcct gtttgattat ctctttaccc atcgtaataa acacaaactg
    19081 atggcgttga ttgatgttcc gcagatgaaa cccttggttc acgtttccgg aatgtttggg
    19141 gcatggcgcg gcaataccag ctgggtggca ccgctggcgt ggcatcctga aaatcgcaat
    19201 gccgtaatta tggtggattt ggcaggagac atttcgccat tactggagct ggatagcgac
    19261 acattgcgcg agcgtttata taccgcaaaa gccgatcttg gcgataacgc cgccgttccg
    19321 gttaagctgg tgcatatcaa taaatgtccg gtactggccc aggcgaatac gctacgcccg
    19381 gaagatgccg accgactggg aattaatcgt cagcattgcc tcgataacct aaaaattctg
    19441 cgtgaaaatc cgcaagtgcg tgaaaaagtg gtggcgatat tcgcggaagc cgaaccgttt
    19501 acgccttcag ataacgtgga tgcacagctt tataatggct ttttcagtga cgccgatcgt
    19561 gcagcaatga aaattgtgct ggaaaccgag ccgcgtaatt taccggcact ggatatcact
    19621 tttgttgata aacgaattga aaaactgttg ttcaattatc gagcgcgcaa cttcccgggg
    19681 acgctggatt atgccgagca gcaacgctgg ctggagcacc gccgccaggt cttcacgcca
    19741 gagtttttgc agggctacgc tgatgaattg cagatgctgg cacaacagta tgctgatgac
    19801 aaagagaaag tggcgctgtt aaaagcgctt tggcagtacg cggaagagat cgtct
//
LOCUS       DAZUCC010000079        19709 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZUCC010000079 DAZUCC010000000
VERSION     DAZUCC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.079"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            161..703
                     /gene="yfaZ"
                     /locus_tag="ACHY09_002453"
     CDS             161..703
                     /gene="yfaZ"
                     /locus_tag="ACHY09_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFS3885415.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            803..2005
                     /gene="yfaY"
                     /locus_tag="ACHY09_002454"
     CDS             803..2005
                     /gene="yfaY"
                     /locus_tag="ACHY09_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFS3885416.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLTRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEKQAMEKLWLDVKRVAGQSVIFEGTEGLPSQISRELQTRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2225..3007
                     /gene="yfaX"
                     /locus_tag="ACHY09_002455"
     CDS             2225..3007
                     /gene="yfaX"
                     /locus_tag="ACHY09_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS3885417.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     IWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3022..4227
                     /gene="rhmD"
                     /locus_tag="ACHY09_002456"
     CDS             3022..4227
                     /gene="rhmD"
                     /locus_tag="ACHY09_002456"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFS3885418.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VISFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYNH"
     gene            4284..5573
                     /gene="yfaV"
                     /locus_tag="ACHY09_002457"
     CDS             4284..5573
                     /gene="yfaV"
                     /locus_tag="ACHY09_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3885419.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKEEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5590..6393
                     /gene="yfaU"
                     /locus_tag="ACHY09_002458"
     CDS             5590..6393
                     /gene="yfaU"
                     /locus_tag="ACHY09_002458"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFS3885420.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAHVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAADKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGNY"
     gene            complement(6434..6667)
                     /locus_tag="ACHY09_002459"
                     /pseudo
     CDS             complement(6434..6667)
                     /locus_tag="ACHY09_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6668..7663)
                     /gene="rpnE"
                     /locus_tag="ACHY09_002460"
     CDS             complement(6668..7663)
                     /gene="rpnE"
                     /locus_tag="ACHY09_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFS3885421.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDSEGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKIEGWQ
                     EGKLEGLQEGKLDGLQEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCH"
     gene            complement(7856..9046)
                     /gene="glpC"
                     /locus_tag="ACHY09_002461"
     CDS             complement(7856..9046)
                     /gene="glpC"
                     /locus_tag="ACHY09_002461"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFS3885422.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9043..10302)
                     /gene="glpB"
                     /locus_tag="ACHY09_002462"
     CDS             complement(9043..10302)
                     /gene="glpB"
                     /locus_tag="ACHY09_002462"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFS3885423.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLEALRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWSLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQL"
     gene            complement(10292..11920)
                     /gene="glpA"
                     /locus_tag="ACHY09_002463"
     CDS             complement(10292..11920)
                     /gene="glpA"
                     /locus_tag="ACHY09_002463"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFS3885424.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQRIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTMSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12193..13551
                     /gene="glpT"
                     /locus_tag="ACHY09_002464"
     CDS             12193..13551
                     /gene="glpT"
                     /locus_tag="ACHY09_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFS3885425.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            13556..14632
                     /gene="glpQ"
                     /locus_tag="ACHY09_002465"
     CDS             13556..14632
                     /gene="glpQ"
                     /locus_tag="ACHY09_002465"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS3885426.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14835..15485
                     /gene="inaA"
                     /locus_tag="ACHY09_002466"
     CDS             14835..15485
                     /gene="inaA"
                     /locus_tag="ACHY09_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFS3885427.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKSEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15539..15793)
                     /gene="yfaE"
                     /locus_tag="ACHY09_002467"
     CDS             complement(15539..15793)
                     /gene="yfaE"
                     /locus_tag="ACHY09_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFS3885428.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(15793..16923)
                     /gene="nrdB"
                     /locus_tag="ACHY09_002468"
     CDS             complement(15793..16923)
                     /gene="nrdB"
                     /locus_tag="ACHY09_002468"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS3885429.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(16989..19274)
                     /gene="nrdA"
                     /locus_tag="ACHY09_002469"
     CDS             complement(16989..19274)
                     /gene="nrdA"
                     /locus_tag="ACHY09_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS3885430.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            19402..19545
                     /gene="ypaB"
                     /locus_tag="ACHY09_002470"
     CDS             19402..19545
                     /gene="ypaB"
                     /locus_tag="ACHY09_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFS3885431.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     VIS"
BASE COUNT         4532 a         4880 c         5373 g         4924 t
ORIGIN      
        1 cggtaaactg tgtttatttt ttcggcgttt aattatttaa gaagtattta gttacaataa
       61 gtgactagat cccttttttc gctcattgaa tcctctgcgc caggcagtac aatcccgcag
      121 ctttgggaat tttgattaat tgcttgtggg aaaggggata atgaaaaaaa ttgcgctagc
      181 aggtctggcc ggaatgctgt tggtttctgc atcggtcaat gcaatgagca tcagcggcca
      241 ggcgggtaaa gaatacacca atattggtgt cggttttggg actgaatcga cgggcctggc
      301 gttaagcggt aactggacac ataacgacga cgacggtgac gtcgcgggcg tggggctggg
      361 gttgaatctg cctctcgggc cgttaatggc gaccgttggc ggaaaaggcg tttacaccaa
      421 cccgaattac ggcgatgaag gttatgccgc agcggtagga ggtggtttgc agtggaaaat
      481 tggcaacagc ttccgcttgt ttggcgagta ttactactct ccggattcgc tctccagcgg
      541 tattcaaagt tatgaggaag cgaatgctgg cgcgcgttac accattatgc gtccagtcag
      601 tattgaggcg ggttatcgct acctgaacct ttctggtaaa gacggtaacc gcgacaacgc
      661 agtggctgac ggcccgtatg tgggggttaa cgccagtttt tgatcccttc cggcgcggca
      721 ttctgccgcg ccattcagtt tcttactacg ccttatctcc ctttgcgtta tagtgattcg
      781 ataataaaag caggagataa ccatgttaaa agtggaaatg ttatccaccg gggatgaagt
      841 gttacacggg caaatcgttg acactaacgc tgcctggctg gccgattttt tctttcatca
      901 ggggttgcct ttaactcgcc gcaatacggt gggggataac cttgatgact tagtgaccat
      961 tcttcgcgaa cggagtcagc acgccgatgt gctgatcgtt aacggcgggc tggggccgac
     1021 cagtgatgat ttaagcgcac tcgccgctgc gacagcaaaa ggtgaaggcc tggtgctgca
     1081 tgaggcctgg ctcaaagaga tggaacgcta tttccacgaa cgtggacgag taatggcacc
     1141 gagcaaccgt aaacaagcgg agctgcctgc cagtgctgaa tttatcaata acccggtagg
     1201 caccgcctgt ggttttgccg tgcagcttaa tcgttgcctg atgttcttta cccccggcgt
     1261 accgtcagaa tttaaagtga tggtcgagca cgaaatcctg ccgcgcctgc gcgagcgttt
     1321 ttctttaccg cagccgccgg tttgtctgcg tttgactaca tttggtcgtt cggaaagcga
     1381 tctggcacaa agcctggaca ccctgcaact gccgccgggc gtaacaatgg gctatcgctc
     1441 ttctatgccg attatcgaac tgaaactcac cggaccggca agcgagaaac aggcgatgga
     1501 aaaactgtgg ctggacgtta aacgtgttgc cggacagagc gtgattttcg aaggtaccga
     1561 aggactgccc tcgcagatca gccgcgaatt gcaaacccgc cagttcagcc tgacgttgag
     1621 cgagcaattc acaggtggtt tattggcttt gcaactttct cgcgcaggtg ctccattgct
     1681 ggcgtgtgaa gtggttcctt cacaggagga aaccctggcg caaactgcgc actggattac
     1741 agaacggcgg gccaaccatt ttgccgggct ggcactggct gtttcgggtt tcgagaacga
     1801 gcatctcaac tttgcgctag ccacgccaga tggcactttc gctctgcgtg tgcgtttcag
     1861 cactacacgc tacagcctgg ctatccgtca ggaagtgtgc gcaatgatgg cactgaatat
     1921 gctgcgccgt tggttaaacg gccaggacat cgccagtgag catggctgga ttgaggttgt
     1981 tgagtccatg accttatctg tctgattccg ccactctgtg ccggtttact gcaccggcac
     2041 attccccctc cggcaaaatt tgtccaacct gttgtactca ataagaaaaa atccgtgcgc
     2101 taattcacaa atttgtgcgt aatttctcca gtccgctgga cgaggagcgg gatttgatga
     2161 gcttcacaat tttactccac ctgagcaacg agactgattc cagtatctaa gactggagtc
     2221 cagtatgttg gaatcaagca aagttcccgc cctgacacgg gctattgata ttctcaattt
     2281 gattgcccgc attggcccct gtagtgctgc gacgatcatt gacacattgg ggatccctaa
     2341 aagcacggcc tatttgctgc ttaatgaact caggcgtcag cgttttctta gtcttgatca
     2401 ccaggaaaat ttttgtttgt ggaccaggct ggtggagctt tccggtcatg cgttaagcaa
     2461 gatggatctc cgggaactgg ctcgaccgcg tttgacgcaa ctgatggata ccaccgggct
     2521 gttatgtcac ctggggatta ttgataacgg cagcgcttat tacattttga aagtggagtc
     2581 atcagccact atcagcgtgc gttcccatga aggaaaaagc ctttcgcttt atcgctccgg
     2641 aataggtaaa tgcctgcttg cctggcaacc tgcagctgta cagcaaagta ttatcgaagg
     2701 gctgatctgg gagcaggcga cacccacaac gattactcat ccacagcaac ttcacgaaga
     2761 actggcgcgt attcgccgtc agggctggag ctatgacaac ggcgaagatt acgccgatgt
     2821 ccgttgtgtc gctgcgccgg tgtttaacgc caataacgag cttaccgctg cgatctcggt
     2881 ggtcggtacc cgcttacaaa tcaatgaaga ataccgtgat tatctggcgg gcaaggccat
     2941 tgcctgcgcc agggatattt cacgtctgtt gggatggaaa agtccctttg acttacaggc
     3001 ctcataacaa tggagaacag catgacccta ccaaaaatca aacaggttcg cgcctggttt
     3061 actggcggtg cgacagcaga aaaaggcgct ggcggcggtg attatcacga ccagggggca
     3121 aatcactgga ttgatgatca tattgccacc ccgatgagta aataccgcga ttacgagcag
     3181 tcacgccagt catttggcat taacgttctt ggcacgttga ttgttgaagt cgaagcagaa
     3241 aacggccaga ccggattcgc cgtttcgaca gccggtgaaa tgggctgttt tattgtcgaa
     3301 aaacacctta accgtttcat tgagggtaaa tgtgtcagtg atatcaaact gatccacgat
     3361 caaatgctca atgccaccct gtattactcc ggctctggtg gcctggtgat gaacacgatt
     3421 tcctgtgtcg atctggctct gtgggatctg ttcggcaaag tggtcgggct tccggtttat
     3481 aaacttttag gcggcgcagt tcgtgatgag attcagttct acgccacagg tgcgcgtccg
     3541 gatctggcaa aagagatggg ctttatcggt ggcaaaatgc cgacgcactg ggggccacat
     3601 gatggcgatg cggggatccg caaagatgcc gctatggtcg cggatatgcg tgaaaaatgc
     3661 ggtgaggatt tctggttaat gctcgactgc tggatgagtc aggacgtgaa ctatgccacc
     3721 aaactggccc acgcttgcgc gccctataac ctgaaatgga tcgaagagtg cctgccgcca
     3781 cagcagtatg aaggttatcg cgaactgaaa cgcaacgcgc cagtcggaat gatggtcacc
     3841 agcggtgagc atcacggcac actgcaatct ttccgtacgc tttcagaaac cggtatcgac
     3901 attatgcagc cggatgttgg ctggtgcggt ggcttaacca cgctggtgga aattgccgca
     3961 atcgccaaat ccagagggca actggtggtg ccgcacggtt cgtctgttta ctctcaccat
     4021 gcggtgatca gcttcaccaa tacgccattc agcgaattcc tgatgaccag cccggattgt
     4081 tcaacgatgc gtccgcaatt tgatccgatt ctgcttaatg agccagtgcc ggtgaatggt
     4141 cgtattcata aatcagtgct tgataaaccc ggtttcggcg tcgaactcaa tcgtgactgc
     4201 aatttgaaac gcccctacaa ccactaatca ctccgggcgt tgctgtgaca acggtaacgc
     4261 ccacatattc taaggagctt tttatgagca ccgctttgct tgacgccgtg gtgaagaaaa
     4321 accgtgcgcg tttaattccg tttatgttgg cgctgtatgt gctggcgttt ctcgaccgtt
     4381 cgaatatcgg ttttgccaaa cagacctacc agattgatac cgggctgagt aatgaagctt
     4441 atgcgctggg agcaggcatt ttctttgtgg tatatgcgtt tctaggtgtt ccggcgaatc
     4501 ttttgatgcg caaactgggg gccagaacct ggattggtac gacaacactg ctgtggggat
     4561 ttctttcggc agccatggca tgggccgata ctgaagcgaa atttctgatc gttcgcactc
     4621 tgcttggtgc tgcggaggcc gggttcttcc ctggtatgat ttatctcacc tcgcaatggt
     4681 ttccgcagcg taatcgcgcc agcattatgg ggctgttcta tatgggcgca ccgttggcgt
     4741 taacactggg atcaccgctt tccggcgcgc tgctggagat gcatggattt atgggacatc
     4801 ccggctggtt ctggatgttt gtgattgaag gattgctggc agtcggcgct ggggtattca
     4861 cattcttttg gcttgatgac acaccggagc aggcacgttt tctgagtaaa gaagaaaaaa
     4921 cattgcttat caatcaactg gcaagtgaag aacaacagaa agtgacttct cgactgagcg
     4981 atgcgctgcg taatgggcga gtctggcaac tggcgattat ctacctgacc attcaggtgg
     5041 cggtttacgg attaattttc ttcctgccga cccaggttgc tgcattgctg gggacaaaag
     5101 tgggctttac ggcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct
     5161 ggcttattcc gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat
     5221 tactggcggc aggcattggt attggtctct ccgggctgct ttctccagta ctggcgatcg
     5281 tagcgctgtg tgttgcagcc atcgggttta ttgccgtgca gccggtgttc tggacgatgc
     5341 cgacacaact gctttccggt acggcgctgg cggcggggat tggttttgta aacctgtttg
     5401 gtgcagtggg tgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttgtttgcca
     5461 gcgatgcggc gggattactg acgctggcag cggtggcggt catcggttcg ctgattattt
     5521 tcactctgcg tgtaaatcgc actgttgcgc agaccgacgt ggcacatcat taataggtta
     5581 aggaacacga tgaacgcatt attaaccaat ccctttaaag aacgtttacg caagggcgaa
     5641 gtgcaaattg ggctgtggtt aagctcaacg actgcctata tggcagaaat tgccgctact
     5701 tccggttatg actggttgct gattgacggg gagcacgcgc caaacaccat tcaggatctt
     5761 tatcaccagc tacaggcggt agcgccctat gccagccaac ccgtgatccg tccggtggaa
     5821 ggcagtaagt cgctgattaa acaagtcctg gatattggcg cgcaaactct actgatcccg
     5881 atggtcgata ctgccgatca ggcacgtcag gtggtgtctg ccacgcgcta tcctccctac
     5941 ggtgagcgtg gtgtcggggc cagtgtggca cgggctgctc gctggggacg cattgagaat
     6001 tacatggcgc acgtcaacga ttcgctttgt ctgttggtgc aggtggaaag taaaacggca
     6061 ctggataacc tggacgaaat cctcgacgtc gaagggattg atggcgtgtt tattggacct
     6121 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     6181 attattgaaa ccagcattcg gcggatccgt gctgcggata aagcggctgg ttttctggct
     6241 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     6301 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     6361 aaaaatgggc cacgcataaa aggtaattat tgatatcaaa ggcccatggg ggtcggctgt
     6421 gggcctgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggctg
     6481 cgagcaccag gtcacgatca atgccctgta ccagcatgcg caaagcaatt ttaatggctt
     6541 gtttacgcat accttcctgc aaaccttcta atttaccctc ctgccagcct tcaagcttac
     6601 cttcctgcca gccaatctga tgcccttcct gcctcagccg ttcagcaata gtcatcaatc
     6661 tctcctttta atgacagttc ggaatatcag catcagtgag ttgggttgtc gccagcacaa
     6721 tctcacgttc aaagccctgc tccagcatgc gcaaggcaat tttaatggct tgttcatgca
     6781 tgccttcctg caaaccatct aatttacctt cttgcaaacc ttctaattta ccttcctgcc
     6841 aaccttcaat tttaccttcc tgccagcctt caatcttacc ttcctgccag ccaatttgat
     6901 gcccttcctg ccgcagccgt tcagcaatag tcattaatct ctccttttgt agtggtgaac
     6961 gttcggcaat ctcctcaata aaacgggtga aacgggaggt atcgccgcat tgcagcagat
     7021 aattaaacag cgtttgtagc tggctgtcat tagtgaagcc tctaaccaaa agcgtggtga
     7081 tcctgtcgac cataccgatt aaatcgcggt cgcgaatatg cttttgaatc agttcaagca
     7141 gagctatacg ccgatgttgc atgatctcat cgtcaggtac gatggtgata tccaccaacg
     7201 gaaaagcttc ggtgtacaac tgccgggcaa gttgcggatc gtcaaactca tccagccagt
     7261 tgagtgagta cgggtagggc gaggtttcgc catgataaaa cagcaatggc accaccagcg
     7321 gaactctgtc atagccttta tccaggtgac gctgcatggc ggcagtggca tagcgcatta
     7381 gccgaaaagc catattcttt tctgcagagc tttgatgttc aatcacgcag tagatatagc
     7441 cttcactgtc gctggtttcc acagaccaca aaacatccga gtagtaagcg cgtaaacttt
     7501 tttcaataaa gctggtgggt tccaggcgta aagtttgcag gttgcaaagc ttgcgcagcg
     7561 gctctggtag atgtatttcc agaaaatccc gagcggtttc gggtgtgaac ataaaggttt
     7621 taaacaccgc atcatgcggg gaggaggttg ttgattcggt catggttgtc cgtcacctga
     7681 tatttatgat gacgcgacat tacggttatc ccaggccttt gacatctgcc agtttcagcc
     7741 tttgcgagac gttttcagag gaattttcac tgcgttgcaa cgcatacatt gggcacggaa
     7801 tcgtgtacgc aaaaatgccc ggtaacaaca tgcttaccgg gcatcagaaa ggagtttaag
     7861 ccagcgcctg ggccagcagc gtaatcggat gttcgcagcg aagacttgtg gacatctcaa
     7921 tctgccattt gcaggtttcg cagtcggtaa ccaccagatc tgcgccgctt tcttctatct
     7981 gacggaacag tggtgcgccg atggcttgtg aggtggggta gttctctttt ttgaaaccgt
     8041 aagtacctgc aataccgcag cactgggaat ccagcaccgt taactcaagc cccgggattt
     8101 tacgcaacag ctccagggtg tagagcgtcc agcccatttt ttccatatgg cacggagtgt
     8161 gataaaccac tttcagcggc agcggtttca gcggcaaggt tttgccttcg tccagcttgc
     8221 gccacagcca gcgggtcgcc agttcgatgt gatcgcgcaa gcctttgttg tcgacattca
     8281 gcacttccgg atattcgtcg cgcagggcaa atgtacaggt tgaggaggtg gcaatcaccg
     8341 gaatgccttt tactcccact gcttcgcgga tcgactctac atttgtgatt gcctgtttac
     8401 gtgctttatc ggtaaagccg ttggcgatca gcggtacgcc gcagcatttt tctttgctga
     8461 gcagttgtac accggtaccc attgcgttga gcactttaat taaatctttg cctaactgcg
     8521 gatggttgta gttaacgaag cagccgtgaa agaaagccac ctggtcttta tattgtgcct
     8581 gctgggccgc cacgctgcga taccagcgac ggaacgtacc gaaggagtat ttcggtagcg
     8641 tgcggcgatg atcgattttt aacgccgcat caagcagctg ccgcactggt ttcagcgagg
     8701 tagcggtatt gacgattggt gcgaatggcg tggaaacgct acccatcagg tcggtatgac
     8761 tcaacacaaa attgcgcagc gacgggcgtg tggtgtcata tttcgcccgc gcgcgctgga
     8821 tgatatcgcc aatcttcaca tcggacgggc aggcgacttc gcagcgtttg cagttgatgc
     8881 aatatttcag cgcttcgtca tacagtgcgc catctttcag acgcagacgc tcgccatccg
     8941 gcccggcttg ttttggccct ggatagccgg gattcacccg gctcaccggg caggcggtgg
     9001 tgcagacggt gcacttaatg cagttttcga agctggtgtc gttcatagtt ggcctcctgc
     9061 gcgttgggca atctgttgag cggcatgtaa agcactgacg gcacaaacac cgccgccgca
     9121 tccctgggcg atgggatcaa atccgcccag caccgaaccg atggcaaaca ggttctcaat
     9181 ggtttgccct gcctgcgatg ggcgtagtgc ctcatcagtg gttacaccga acagctgcca
     9241 cggttgcggc gcaaaaaaat cgcccttata ccattcaccc cgcgtggcgg tttgtagcac
     9301 atcaaggccg agaatcggct cgcgaatgcc gtcacgttct gccaccagtc cgccgctaaa
     9361 gaaactgccg ctcgcaagca ccgcaaatcg tgggcgcagc gggatatcgg cgtgattgcg
     9421 cgtccagatt tcattcacta cgccattttt acaggtcact tttttcactt catcgcctgg
     9481 catccacacg ccaccctggc gcacaaactg gcgctgtaac tggttttgca gacgaatgcc
     9541 cagcacggaa ggcggcagcg ttggcaaaag catcagtgaa caaggtagtt tttcattcaa
     9601 ccaacgccac agtttgtcat cggccaggcc gaagcaggcg ggcatcagga tcatttcgca
     9661 ggtattggcg acaggaataa gcgcatcaag taacagcgac cagttctctt cgttatcaag
     9721 gaaacgggcg atattcaccg cgcgaaactc ggtggcgttg ttgcgcagca catccagttc
     9781 cggcagctct atttctgcgg tttcaacggc aaggtcgagt tcgcgcaacg aagccgccgc
     9841 aagatgcgcc tgaaaatcca tcaggccgct tatcccgacc acacatattt tcttcgcggg
     9901 caacggccag acaggaactt ctggcgaact tagccaggtt gagcgcagag tgccgagcgg
     9961 ggtaactcgc tggtgggcca gttctacgct gccctgtaat tgcgcaccgc tttcagcgat
    10021 taatgcctgc gcctggcaag cgagatcgag tacgcgttgt ggcccgagaa gagtgtaagg
    10081 atgggcaggt gcctgctgac gcaaagcttc cagtccactg tgaatgtctg tcaccggttg
    10141 accatccggc agatggctca gcaaatccag cgatccagat gagaaatgga gcgcgctttg
    10201 accacgcgtg acaatggcac agcgcaggcc gtgtttttgc agttgcaggc cacagagtaa
    10261 tccggcgagg ccgccgccca taatgacagt atcaaagcgc atctttctgc tccttctcca
    10321 gaccacacag tccctgataa acccagcggg taaattcgct ttcgcgcagt gcatctcccc
    10381 aggcgatggg ttgcacgcct ttccagcgtt cgttaaggaa ggtggaaagt tgctcgatag
    10441 attgcgcgga catcgtgacg ttaaaacgtt gcagcagtcc ggcagcgcgg caggcgcaga
    10501 gttcgccctg gcaggtaccc atccccacac gggtacgacg gcgtaaatcc agcaggctat
    10561 taacgtttaa attttctacc gcgtactgaa cttcacccgc agttaccgct tcgcactcac
    10621 ataccaggct acggtgcaga cggccttcgc tcagccaggc gggtgtgcga tcgccatgac
    10681 gataaaccgc agaaccgcgc agcggggcag gcagggagat gactttacgc aaggtaactt
    10741 cagcgggctc ctgtgaacca ggaagtgcca gatcggcggt tgtacagggg cgcgtgttgc
    10801 ccagcttgcg acataccgcg tcggtagccc attccgccat cagccgatag gtcatcagtt
    10861 tgccgccggt gatggtgata aatccgtcca gaccatcgcg ttcagcatgg tcgagcagca
    10921 cgatgccacg actgacgtta cggccgctcg ggtcgtcatc gctggcaacc agcggacgca
    10981 cgccagagta agcccgcaaa atacgcgttt tcgccatcac gggggccagt ttttcccctt
    11041 cacgcagcag aatatcaact tcttctgccg tcactcgatt atcgtcaatc tcattgtaat
    11101 caatacgtaa agaggtggtg ccaattagcg aaatggtatc gccaggcacc agaatatcgg
    11161 cgtcggaagg tttgcggcag cggttgatca catgctgatt aatgcggtga tccatgatca
    11221 gcagcgatcc tttcgccggg aacatgcgaa tgcgcagatc ggcatattcg gcaatgcgtt
    11281 gcccccagat cccagcggca ttaaccacga caggcgcatg aagggcctga gtttcgccgg
    11341 tgagatggtt acgtacacga acgccgcaca ccgtcgcacc ttcacgaatc agccccgtga
    11401 cttcatgagc ggtaaggata acggcaccgt gttctttggc atccagcatg tttgctgcgg
    11461 tcagacgaaa tggatcaacg gtgccatccg gaactttcac cgcgccaatc agtgccgggt
    11521 taacggcagg ttcgataatg cgcgcttgct gcgggtctat agcttctgcg ctgatccctg
    11581 cttcttcgca ggcgcggata aaagtggcct ggaaggagag gtcatcttcc ggcagggtga
    11641 taaacaggcc gttggtcggt tcaacgcagt gacgtgcaat gcgtttcagg atctggtttt
    11701 cactaataca ttcgcgggcc gattccgcat cggttaccgc atagcgcgca ccgctgtgca
    11761 gcaggccgtg gttacgcccg gtggcaccgg ttgcgatgtc gtggcgctca accaaaatca
    11821 cgcgcagtcc gcgcagggca cagtcgcggg caatcccggc tcccgttgcg ccgccgccaa
    11881 tgataatcac gtcacttgat tgcgagtcgc gagttttcat tgttatccct ctgaagttcg
    11941 ttttttacca tttagccata gtaaaaacat gaattgtttg atttcgcgca tattcgctca
    12001 taattcgaaa gtgaaacgtg atttcatgcg tcattatgaa catttaaccc atcgaattta
    12061 ataatgtgtg cggtaattca catttaattt atgaatgttt tcttaacatc gcggcaactc
    12121 aagaaacggc aggttctccc actgaatcag gctgttaatc ataaataaga ccacgggcca
    12181 cggaggctat caatgttgag tatttttaaa ccagcgccac acaaagcgcg cttacctgcc
    12241 gcggagatcg atccgactta tcgtcgattg cgctggcaaa ttttcctggg gatattcttt
    12301 ggttatgctg cttactattt ggttcgtaag aactttgccc tcgctatgcc ttatctggtc
    12361 gagcagggat tctcacgcgg tgatttaggt tttgcccttt cggggatctc gattgcttat
    12421 ggattttcga aattcatcat gggctcggtg tcggatcgct cgaatccgcg cgttttcctg
    12481 cccgcaggtt tgattctggc ggcggcggtg atgcttttta tgggctttgt gccgtgggcg
    12541 acgtcgagca ttgcggtgat gtttgtgctg ctgttcctct gcggttggtt ccaggggatg
    12601 ggatggccgc cgtgcggtcg tactatggtg cactggtggt cgcagaaaga acgtggcggc
    12661 attgtgtcag tgtggaactg tgcgcataac gtcggtggtg gtattccgcc gctgctgttc
    12721 ctgctgggga tggcctggtt caatgactgg cacgcggcgc tctatatgcc cgctttctgc
    12781 gccattctgg tggcactgtt cgcctttgcg atgatgcgcg ataccccgca atcctgtggc
    12841 ctgccgccga tcgaagagta caaaaatgat tatccggacg actataacga aaaagcggaa
    12901 caggagctga cggcgaagca aatcttcatg cagtacgtgc tgccgaacaa actgctgtgg
    12961 tacatcgcta tcgccaacgt gttcgtttat ctgctgcgtt acggcatcct cgactggtca
    13021 ccgacttatc tgaaagaggt gaaacacttc gcgctggata aatcctcctg ggcctacttc
    13081 ttctatgagt atgcgggcat tccgggcacc ctgctgtgcg gctggatgtc ggataaagtc
    13141 ttccgtggca accgtggggc caccggcgtc ttctttatga cactggtgac catcgcgact
    13201 atcgtttact ggatgaaccc ggcaggtaac ccaaccgtag atatgatttg tatgattgtt
    13261 atcggcttcc tgatctacgg ccctgtgatg ctgatcggtc tgcatgcgct ggaactggca
    13321 ccgaaaaaag cggcaggtac ggcagcgggc tttaccgggc tgtttggtta cctgggcggt
    13381 tcggtggcgg cgagcgcgat tgttggctac acagtggact tcttcggctg ggatggcggc
    13441 tttatggtaa tgattggcgg cagcattctg gcggttatct tgttgattgt tgtgatgatt
    13501 ggcgaaaaac gtcgccatca ggaattactg caaaaacgca acggaggcta atggcatgaa
    13561 attgacgctg aaaaacctta gcatggcgat catgatgagt ggaatgatca tgggaagcag
    13621 tgcaatggcg gcggacagca acgaaaaaat agtcatcgcc catcgcggtg ccagtggata
    13681 tttgccggag catacgctgc cagcaaaagc gatggcgtat gcgcagggag cggattatct
    13741 ggaacaagat ttggtgatga ccaaagacga ccatctggtt gtcctgcatg accattatct
    13801 cgatcgtgtt actgatgttg ccgatcgttt cccggatcgg gcgcgcaaag acggtcgtta
    13861 ctacgcgata gatttcacgc tcgatgaaat taagtcactg aaatttaccg aaggtttcga
    13921 tattgaaaat ggtaagaaag tacagactta tccggggcgt ttcccgatgg gtaaatctga
    13981 cttccgggta cacactttcg aggaagagat tgaatttgtt caggggttaa atcactctac
    14041 cgggaaaaat atcggtatct atccagaaat taaagcgccg tggttccatc atcaggaagg
    14101 gaaggatatt gcggcaaaaa cgctggaagt gctgaagaaa tatggttaca gcggtaagga
    14161 cgacaaagtt tatttgcaat gttttgatgc cgatgaactg aagcgtatta agaatgagct
    14221 ggaacctaaa atgggcatgg acctcaatct ggtacagctg attgcctata ccgactggaa
    14281 cgaaactcag cagaaacagc cggacggaag ctgggttaat tacaactacg actggatgtt
    14341 taagccgggg gccatgaaac aggtggcgga atatgctgat ggtattggtc cggattatca
    14401 tatgttgatt gaggagacat cgcagccagg taatatcaaa ctcactggca tggtgcaaga
    14461 tgctcagcag aacaagctgg tagtgcatcc ttataccgtg cggtcagata aactgcctga
    14521 atacactact gatgtgaatc agttatatga tgctctgtat aacaaagcgg gtgtaaatgg
    14581 gctatttact gatttccctg ataaggcagt aaaattcctt aataaagagt aaattaacta
    14641 ccggatggca cttatgtgct atccgatacc actcttcatt aaagtctgtg ggttatcctt
    14701 aattgcaatt attttgagcg agatgacttg ttcccggcat aagatactcg tcaatacaac
    14761 acgtattatt aagattttcc tcagctaaac atctatagtc attgtcgtta ataaaaaacg
    14821 acaggcgata gggtatggca gtttcagcaa agcacgacga atttaatcac tggtgggcaa
    14881 cagagggcga ctgggttgaa gagcctaact atcgccgtaa tggtatgagc ggggtgcaat
    14941 gcgtcgagcg caacggcaaa aagctatatg taaagcgcat gacgcatcat ctgtttcatt
    15001 ccgtacgtta tccgttcggc cgaccaacga ttgtccgtga ggttgcggta attaaagaac
    15061 tggaacgggc cggtgtcatc gtgccgaaga tcgtttttgg tgaagcggtg aaaattgagg
    15121 gggaatggcg cgcgttgctg gtgactgaag atatggcggg gttcatcagc attgctgact
    15181 ggtatgccca gcatgcagta acgccctatt ctgacgaagt acggcaagcc atgttgaaag
    15241 cagtggcgct ggcgtttaag aaaatgcata gcgttaatcg tcagcatggc tgttgctatg
    15301 ttcgccatat ctatgtgaaa acagaaggta agtcagaagc tggttttctt gatctggaga
    15361 aaagtcgtcg ccgcttgcgt cgcgataaag cgattaatca tgacttccgc cagctggaaa
    15421 aatatctgga gccgatccct aaagcggact gggagcaggt gaaagcgtat tactacgcca
    15481 tgtaatcgtt gtaggccaga taatacgcgt aagcgtcgta tctggcgcta gtacccgatc
    15541 acaactcgat ttcaatatcg ccttttgccc ggcaacaaca gggcaaaatt tcccccggct
    15601 gaataaaggc taacggttcg gcaatccagt ccacctggcc tgcaacaagg cgcgtgcgac
    15661 aggaaccgca gtaaccttcg cggcactggt actcaaccgc cacattgtgg gattccagcg
    15721 ccgccagaag ggaagggtgt tcatcctggc acagcagttg tgtgccagtg atgcgcaggg
    15781 taacgcgagc catcagagct ggaagttact caaatcgtcg gtgtccactt ccgagtcaat
    15841 ctgcccgacc agataagaac tgacttccac ttcctgcgga gcaacctgca cgttatcaga
    15901 caccagccag gtattgatcc acgggatcgg gttggagcgt gtctggaacg gcagatccaa
    15961 accgactgcc tgcatgcgga tattggtgat gtattcaacg tactggcaga gaatgtcttt
    16021 attcagacca atcatcgaac cgtcacggaa cagataatcc gcccagtctt tctcttgctg
    16081 tgccgcctgg acaaacaggt catagcactc ctgcttacat tcttcggcaa tttccgccat
    16141 ctccggatca tccgcgccgc tgcgcagcag attcagcata tgctgagtac cggtcaggtg
    16201 cagggcttca tcgcgagcaa tcaggcgaat aattttggcg ttaccttcca tcagttcgcg
    16261 ttctgcaaag gcaaaggaac aagcaaagct gacgtagaag cggatcgctt ccagcgcgtt
    16321 aacgctcatc aggcagagat acagtttttt cttcagctcg cgcaggctaa cggttacggt
    16381 tttaccgtta acggtgtggg taccttcgcc cagcagatgc cagtagctgg tcatttcgat
    16441 cagctcatcg taatagctgg agatcccttc cgcacgtttc tggatctgct cgttggtgac
    16501 gatatcgtca aacacaacag acggatcgtt aacgatatta cgaatgatat gggtataaga
    16561 acgagagtga atggtttctg agaacgccca ggtttcgacc caggtttcca gctcagggat
    16621 cgagatcagc ggcagcagtg ccacgttcgg gctgcgaccc tgaatggagt ccagcagcgt
    16681 ctgatatttc aggttgctga taaagatgtg tttttcgtgt tcaggtagtg cctggtaatc
    16741 gatacggtca cgagaaacgt caacttcttc cggacgccag aagaaagaga gctgcttttc
    16801 gatcagcttt tcgaagatgt catatttttg ctgatcgtag cgagccacgt taaccggctg
    16861 accaaagaac atcggttctt tcagctgatc atttttcgtc tgtgaaaagg tggtatatgc
    16921 catgaatgtg tcctgttggg agtttaatgc cggataaggc attttacgcc gcatccggca
    16981 tctcaatatc agatcttaca tgcgccgctt tcgcagccat cgtcctggat tgacggtacc
    17041 agatcgtctt gtgcgtcttc agcaccgtca cgggtgttct gataatacag cgttttgacc
    17101 ccgaatttgt aggcggtgag caggtctttc agcagttgct gcatcggcac ttttcctgac
    17161 gggaagcgtg acggatcgta gttggtgtta gcagagatcg actgatcgat aaatttctgc
    17221 atgataccca ccagttgcag gtagccatcg ttacccggca tttcccacag cagttcatag
    17281 gcgtcgtgca gatgctcgta gtccggcacc acctggcgca gaataccgtc tttcgacgct
    17341 ttgatgctga cgtaaccacg cggcggttca ataccgttag tggcgttaga gatctgcgaa
    17401 gaggtctcgg acggcatcag ggcagaaagc gtggagttac gcaggccgtg cgttttgatt
    17461 gactcacgca gtgcttccca gtcgtaatgc agcggctcat tagcgatggc atccagatct
    17521 ttcttatagg tatcgatcgg caggatccct ttcgcgtaag tggtttcgtt aaaccacggg
    17581 cacgcgcctt gctctttcgc cagctcatta gacgctttca gcaggtaata ctgaatggct
    17641 tcgaaggttt tatgcgtcag gttgttggcg ctgccgtcgg agtagcgttt accgtgcttc
    17701 gccaggtagt aagcgaagtt gatcacacca atacccagcg tacgacgacc catcgctcca
    17761 cgtttggcgg ccgggatcgg gtaatcctga taatccagca gcgcgtcgag tgcgcgtact
    17821 gccagaattg ccagctcttc cagttcatcc aggctgttaa ttgcgcccag gttgaaagca
    17881 gacagcgtac acagcgcgat ttcaccgttc tcgtcgttga cgtcgttcag tggtttggtc
    17941 ggtagggcga tttccaggca caggttagac tggcgcactg gtgcaatagc cggatcaaac
    18001 gggctgtggg tgttgcagtg gtctacgttc tgaatataga tacgaccggt agacgcacgt
    18061 tcctgcatca tcagcgagaa cagctcaacg gctttcacac gctgcttgcg gatgctgtcg
    18121 tctttctcat atttggtgta caggcgttca aactcttcct gatcggcgaa gaacgcgtcg
    18181 tacagccccg gtacgtcgga cgggctgaac agggtgatat cttcaccttt cagcagacgg
    18241 gtatacatca gtttgttgat ttgtaccccg tagtccatat gacgcacgcg gttgccttca
    18301 accccacggt tgtttttcaa caccagcagg ctttccactt ccagatgcca catcgggtag
    18361 aacagtgttg ccgcaccgcc gcgcacaccg ccctgagagc aggatttcac cgctgtctgg
    18421 aaatgtttgt aaaacggaat acagccggtg tggaatgctt cgccaccgcg aatcgggcta
    18481 cccaacgcac gaatacgccc ggcgttgatg ccgatcccgg cacgctggga aacgtattta
    18541 acaatcgcgc tggaggtggc gttgatggaa tccaggctgt cgccgcactc gatcagtacg
    18601 caggagctga actgacgagt cggggtacgc acgccggaca tgattggcgt cggcagcgaa
    18661 attttaaatg tggaaaccgc gtcgtaaaaa cgcttcacgt attgcaggcg cgtttcacgc
    18721 gggtagttcg agaacaggca cgcggcaacc agaatataaa ggaactgggc gctttcgtag
    18781 atttcgccgg tcacgcggtt ctgtaccaga tatttgcctt ccagctgctt aacggcagca
    18841 taagagaagg tcatatcacg gtcgtgatcg ataaacgtgt ccatctgctt gaactcttct
    18901 tccgtgtagt cttccagcag atgattatcg tatttgccca tctcgaccat tttcaccacg
    18961 tggtcgtaca gcgcaggcgg ctcaaactgg ccgtaggctt ttttacgcag gtggaagatc
    19021 gccaggcgcg cggcgagata ctgataatcc ggcgcatcac gggagatcag gtctgcggca
    19081 gccttgataa tggtttcgtg gatgtcagag gtcttgatac cgtcataaaa ctgaatgtgg
    19141 gagcgcagtt cgacctggga aatcgaaacg ttatgcagtc cttctgccgc ccaatccaga
    19201 acgcgatgga ttttgtcgag attgatgcgc tctgtgctac cgtcgcgctt tgtcaccagc
    19261 agattctgat tcatgtatgt cgtacctgtt tttggaattc tttccgcaat agtttcatgc
    19321 agactacctg tagtgaggga agctgcttag aacactatat atagggggta tgtttgggat
    19381 tcactgcaag atagtgtgaa aatggccctc ttgcaagtgc ataactttgt ggataactca
    19441 ggaaggaaaa agtggctttc gcgcacctta ggtcagacaa ggtgtccggg aaagtcaatg
    19501 ggaagagaaa aatttgttaa aaataactgt tcggtgattt cttgaacggc agaataggtt
    19561 ggataaattg atatagatgg tttattaatc tttttgctaa tgattgcaaa aattgatcta
    19621 agcacgagtc atcacataat ctgatgtatc agttgtggtc aatatataag cgaatgtaag
    19681 cgattgttga tgtcgaaatg gttgttttc
//
LOCUS       DAZUCC010000080        19625 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZUCC010000080 DAZUCC010000000
VERSION     DAZUCC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.080"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..140
                     /locus_tag="ACHY09_002471"
     CDS             <1..140
                     /locus_tag="ACHY09_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS3885432.1"
                     /translation="FMKWFSNSSENNTLTALRNTLLPKLISGELSVEDLPDHIKQTEA
                     A"
     gene            235..3498
                     /locus_tag="ACHY09_002472"
     CDS             235..3498
                     /locus_tag="ACHY09_002472"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS3885433.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEANIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVIGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            3713..4051
                     /gene="symE"
                     /locus_tag="ACHY09_002473"
     CDS             3713..4051
                     /gene="symE"
                     /locus_tag="ACHY09_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFS3885434.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVIEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQRQVQDF
                     IGVISSKTPR"
     gene            complement(4093..4146)
                     /locus_tag="ACHY09_002474"
     CDS             complement(4093..4146)
                     /locus_tag="ACHY09_002474"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFS3885435.1"
                     /translation="MSDERLRDIGLRREDVE"
     gene            complement(4135..4290)
                     /locus_tag="ACHY09_002475"
                     /pseudo
     CDS             complement(4135..4290)
                     /locus_tag="ACHY09_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001684533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            4433..5845
                     /gene="yjiR"
                     /locus_tag="ACHY09_002476"
     CDS             4433..5845
                     /gene="yjiR"
                     /locus_tag="ACHY09_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS3885436.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(6087..7031)
                     /locus_tag="ACHY09_002477"
     CDS             complement(6087..7031)
                     /locus_tag="ACHY09_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFS3885437.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            7498..8730
                     /gene="mdtM"
                     /locus_tag="ACHY09_002478"
                     /pseudo
     CDS             7498..8730
                     /gene="mdtM"
                     /locus_tag="ACHY09_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
     gene            8771..10051
                     /gene="yjiN"
                     /locus_tag="ACHY09_002479"
     CDS             8771..10051
                     /gene="yjiN"
                     /locus_tag="ACHY09_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFS3885438.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            10167..11318
                     /gene="yjiM"
                     /locus_tag="ACHY09_002480"
     CDS             10167..11318
                     /gene="yjiM"
                     /locus_tag="ACHY09_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFS3885439.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            11328..12095
                     /gene="yjiL"
                     /locus_tag="ACHY09_002481"
     CDS             11328..12095
                     /gene="yjiL"
                     /locus_tag="ACHY09_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFS3885440.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTSFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            12092..12349
                     /gene="ytiA"
                     /locus_tag="ACHY09_002482"
     CDS             12092..12349
                     /gene="ytiA"
                     /locus_tag="ACHY09_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFS3885441.1"
                     /translation="MKEHLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            12414..13274
                     /gene="yjiK"
                     /locus_tag="ACHY09_002483"
     CDS             12414..13274
                     /gene="yjiK"
                     /locus_tag="ACHY09_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFS3885442.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            13342..14520
                     /gene="yjiJ"
                     /locus_tag="ACHY09_002484"
     CDS             13342..14520
                     /gene="yjiJ"
                     /locus_tag="ACHY09_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3885443.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMILIALTLLMPSKKHAIVPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(14533..15087)
                     /gene="kptA"
                     /locus_tag="ACHY09_002485"
     CDS             complement(14533..15087)
                     /gene="kptA"
                     /locus_tag="ACHY09_002485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFS3885444.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDIVVATSDKKRFSYSSDGTCIRAVQGHSTSQVAIGFIEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            15337..16020
                     /gene="yjiH"
                     /locus_tag="ACHY09_002486"
     CDS             15337..16020
                     /gene="yjiH"
                     /locus_tag="ACHY09_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFS3885445.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            16017..16478
                     /gene="yjiG"
                     /locus_tag="ACHY09_002487"
     CDS             16017..16478
                     /gene="yjiG"
                     /locus_tag="ACHY09_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFS3885446.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            16491..17663
                     /gene="iadA"
                     /locus_tag="ACHY09_002488"
     CDS             16491..17663
                     /gene="iadA"
                     /locus_tag="ACHY09_002488"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS3885447.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQMLVKDYSFSLSDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            17728..18639
                     /gene="hypT"
                     /locus_tag="ACHY09_002489"
                     /gene_synonym="qseD"
     CDS             17728..18639
                     /gene="hypT"
                     /locus_tag="ACHY09_002489"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFS3885448.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNREELVIPIQAY
                     AYRMNTRMNPIAERFWRELRELEIEPG"
     gene            18646..19017
                     /locus_tag="ACHY09_002490"
                     /pseudo
     CDS             18646..19017
                     /locus_tag="ACHY09_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
     gene            complement(19065..19448)
                     /gene="iraD"
                     /locus_tag="ACHY09_002491"
     CDS             complement(19065..19448)
                     /gene="iraD"
                     /locus_tag="ACHY09_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFS3885449.1"
                     /translation="MMRQSVQTVLPESTGHNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGNWHRTMPQ"
BASE COUNT         4687 a         4946 c         4944 g         5048 t
ORIGIN      
        1 tatttatgaa atggttttct aattcgtctg aaaataatac cctaacagcc ctccgcaata
       61 ccctgctccc caaactcatc tccggcgagc tatccgtgga ggatctcccg gatcacatca
      121 aacaaaccga agccgcataa cgcattttgc ccctgtaaaa tcaggggctt tctggtaagg
      181 ttttctactg atacgggaat gcttaccaga aattagccag ggttggagca cgatatgagt
      241 ctctctttca gtgaagcaaa attagaacaa gcgatcattg aactgttaca ggatcaggga
      301 tatcaacacc tgatcggcga taatgtccca cgttcaagtc tcgatcaggt cattatcgaa
      361 gacgatctcc gtcattattt agcggcacgc taccagcctg atggcattac tgaagaagag
      421 attcagcgac tgatcaaaca gttcaccacg cttccggcct ccgatcttta tgaaagcaac
      481 aaaacatttt gtcgctggct ggcaaatggt tttctgttca aacgcgacga tcggcaacaa
      541 aaagatctct atattgaatt gctcgacacc cggcatctac ctgccgcact gcgccagata
      601 tttgacgccg aagatgtccc gttgcaacag gctgcggaac tcccgccttc ctatattaac
      661 ccgccgctta accggattaa gattgttaac cagcttaaaa tctccggcaa agataatcag
      721 actcgtattc ctgacggcat tctctatatc aacggtctgc cactggtcgt ctttgaattt
      781 aaaagcgcgg tgcgcgagca ggaagccaat ataggcgatg cctggaagca gctctgtaaa
      841 cgctaccgcc gggatattcc gcaactgttt atctacaacg cgctctgcat tattagcgat
      901 ggcgtaaata accggatggg caacctgttt gcaccctatg aatattttta ctcatggcgc
      961 aaagtcaccg gtaatgaaaa ccgtgaacag gacggaattc catcattgca ctcaatgatt
     1021 caggggctgt ttcatccggt acgtctgctg gatgtgatta aaaactttat ctgcttcccg
     1081 gataaagcca agcacgaagt aaaaatttgc tgccgatatc cgcagtacta tgccgcccgc
     1141 aaactctatt acagcatcaa gcaagcgcgt aaacctttcg gtagcggtaa aggcggtacc
     1201 tactttggcg caacgggctg cggtaaaagc tacaccatgc aatttttaac ccgcttgtta
     1261 atgaaaagcg tggagtttgc cagcccgacc attgttttga tcaccgaccg caccgatctg
     1321 gacgatcagc tttctgcgca aatgtgcaac gccaaaaatt acattggcga cgacaccatc
     1381 gttcctgtta ccagccgtga agatttgcgt aatcaactgg cgggacgcaa tagtggcggt
     1441 gtattcctga caacgatcca taaattcacc gaagacaccg aactcctttc tgaacgcagc
     1501 aatatcattt gcatctcgga cgaagcacat cgcagccagg ttaacctcga ccagaaagtc
     1561 atcgtcgata aagaaagcgg aaaagtgcgc aaaacgtatg gctttgcgaa atacctgcac
     1621 gattcactgc caaatgccac ctatgttggc tttaccggca caccgattga cgcgacgctc
     1681 gatgtcttcg gtgaggtgat cgacagttac accatgaccg aatccgttca ggatgaaatc
     1741 actgtacgca tcgtgtacga aggccgtgcg gctaaagtga tcctggactc cagcaaactg
     1801 gaagaagtcg aaaagtatta cgaagagtgc gcaaacgcag gcaccaatga gtggcaaatc
     1861 gacgaaagca agaaagccac ggctaccatg aatgcggttc tgggtgatga agatcggtta
     1921 aaagccctcg cggaagattt tgctaaacat tatgaaaaac gcgtagccga aggttccacc
     1981 gtaataggca aagccatgtt tgtttgtgcc agccgtgaaa ttgcctggga tttctaccgc
     2041 cagcttaaag ctattcgccc tgcctggttt gaagtgaagc aagcacctga tggcgtcgtc
     2101 ctgacggaac aggaacaaaa agagttaccg ccttctgaaa tggtgaaaat ggtcatgacg
     2161 cgcggtaaag atgacgacga ggcgctttat gatttactgg gcacgaaaga atatcgtaaa
     2221 gagctggata agcagttcaa aaacgctaaa tcgaatttca aaattgccat tgtcgttgat
     2281 atgtggctga ccggttttga tgttcctgaa ctggatacca tctatattga taagccctta
     2341 caaaaacata accttatcca gactatttct cgcgttaacc gtaagctgga aggcaaaagc
     2401 aaagggttag tggtggacta catcggcatt aaaagtcaga tgaaccaggc actggcaatg
     2461 tattcccgca ttgatgccac caactttgaa gatattcagc aatcggtaat agaagttaaa
     2521 aaccatctcg atttgttggg gcaagtcttt tacgaatttg acagtcggga ttattttagt
     2581 ggtgagccac aagcgcaatt atcctgcctc aaccgcgcgg cggaatttgt tctgcgtacc
     2641 cagaaagttg aacgtcgttt tatgggactg gttaaacgca tgaaagccgc ctacgacgtc
     2701 tgctgcggca gcgaagcatt atcacagaca gaacgtgatc atattcacta ttatcttgct
     2761 gttcgttcaa ttgttttcaa actgacgaaa ggtgacgcac cggatgtcac ccagatgaat
     2821 gcacgcgttc gtgaaatgat tgcagaagcg ctaaaagctg atggcgtaga agaaatttat
     2881 tttcttggcg ataaaaaagc ggaatccatc gatatttttg acgaagatta tctggcgcga
     2941 attaacaaga tcaaacttcc ggcaacaaag atccagttat tacaaaaatt actggaaaaa
     3001 gcgatcagcg acttcaagaa agtgaaccag ttgcaaggga ttaacttcac ccgccgcttc
     3061 caggcgatta tagatcgtta taatgagcgg cgagaagatg atgtactcaa cggtgaagaa
     3121 ttcgatacat tcagtcagga aatgaccgat attatctatg atattaaaac cgaaatgggc
     3181 acctgggccg atttaggcat tgatattgaa gaaaaagcct ttttcgacat tcttgctcat
     3241 atgcgcgata aatatcagtt cacctatgac gatgaaaaaa tgctgtcact ggcaaaagag
     3301 atgaaaagcg tggttgacaa cacatcgaaa tatcctgact ggagtaaacg cgatgatatt
     3361 aaagcgaaac tgaaagttga acttattctg cttctacaca agcataagtt cccgccagta
     3421 gcgaatgatg atgtttatat gggtgtgctg gcacaggcag agaattttaa agaacatcat
     3481 atgagtgctt taaattaatt atgaaggccg gatacacttt gttatccggc cattctgaat
     3541 taacgttaat ttctcttcca gatctttcca gtaacgcttt tattattttc tatttttagc
     3601 tggactttcc ctgtatttac tgatgatata tccaggtatt tagcgcggtg cgaatgtgcg
     3661 ccaacacacc cgcaccgctc atcacaatcc ctatttcacg agaatagtaa tcatgactga
     3721 cacgcattct attgcacaac cgttcgaagc agaagtctcc ccggcaaata accgtcaatt
     3781 aaccgtgagt tatgcgagtc gctacccgga ttacagccgt attcccgcca tcaccctgaa
     3841 aggtcagtgg ctggaaaccg ccggttttgc caccggcacg gcggtagatg tcaaagtgat
     3901 agaaggctgt attgtcctca ccgcccaacc acccgccgcc gaagagagcg aactgatgca
     3961 gtcactgcgc caggtgtgca agctgtcggc gcgtaaacaa aggcaggtgc aggattttat
     4021 tggggtgatt agtagcaaaa cgccacgcta agtttctctc cccttcccca tacgggaagg
     4081 gaaattgccc cctcactcca catcctccct gcgtaacccg atatccctca accgttcatc
     4141 actcatctgc tgtacacccg ccgggcctgc atttgccgcc gccaacgcct caccgcctgt
     4201 cagagttgca caagtctgat aaacggcgtt ttggctttgt tttcgtgaaa ttccatctgc
     4261 tgcctcctca tctttcagag gcttcatcat ccgtcttctg gcagttcaca atacagatgc
     4321 aaatattagt tttatttaac atacagacag gttaaaacag catctgcatc cggtttttta
     4381 gcctcatctg taccggtttt tctctctgta tggtccaaat aaggaataca gcatgacgcg
     4441 ttatcaacat ctggcgactc tacttgccga gcggattgag caagggctgt atcgtcacgg
     4501 ggagaaattg ccgtcggtgc gcagcttaag tcaggagcac ggcgtcagta ttagcaccgt
     4561 gcagcaggcg taccagacgc tggagacgat gaagctcatc actccgcagc cgcgttcggg
     4621 ttattttgtc gcacaacgta aagcccagcc gccagtaccg ccgatgacgc gtccggtgca
     4681 gcgcccggtg gaaattaccc agtgggatca agtgctggat atgctggagg cgcatagcga
     4741 cagttccatt gttccgttaa gtaaaagcac gccggatgtc gaaacgccca gcctgaaacc
     4801 gctgtggcgg gagctaagcc gggtggtgca gcataatctg caaaccgttc tcggttatga
     4861 cttgttagcc ggtcagcgag tgttgcgaga gcagattgcc cgcctgatgc tcgacagcgg
     4921 ctcagtggtc accgctgatg acatcattat caccagcggc tgccataact cgatgtcgct
     4981 ggcgttaatg gcggtatgta aaccgggcga tattgtcgca gtcgaatccc cctgttatta
     5041 cggttcaatg cagatgctgc gcggtatggg cgtgaaagtg attgaaatcc caaccgatcc
     5101 agaaactggc atcagcgttg aagcgctgga actggcgctg gaacagtggc cgattaaagg
     5161 catcattctg gtgccaaact gtaataatcc gctgggattt attatgccgg acgcacgcaa
     5221 acgggccgtt ctctctctcg ctcagcgtca tgatattgtg atttttgaag atgatgtcta
     5281 cggcgaactg gcgacggagt atccgcgccc gcggaccatt cattcctggg atatcgacgg
     5341 gcgagtgctg ctgtgcagct cgttcagtaa aagtattgca ccaggcctgc gcgtgggttg
     5401 ggtcgcaccg gggcgttatc acgataaact gctgcatatg aaatatgcca tcagcagctt
     5461 taatgtaccg tccacgcaaa tggcggcggc aacgtttgtt ctggaaggtc actatcatcg
     5521 ccatatccgg cggatgcggc agatctatca gcgcaatttg gcgctttata cctgctggat
     5581 acgggaatat tttccctgcg aaatctgtat tacgcgcccg aaaggcggat ttttactgtg
     5641 gatagaattg cctgaacagg tcgatatggt ttgcgttgcg cggcagctgt gccgcatgaa
     5701 aatccaggtg gcggcaggct cgattttctc agcttccggc aaataccgta attgtctgcg
     5761 catcaactgc gctttgccgc tcagcgaaac ctatcgcgaa gcactgaagc aaattggcga
     5821 ggccgtgtat caggcaatgg aatgaattcg aattagtggc gaggcccctg atttcctctc
     5881 acgaatcgcc tgtgactcgt tttatctttg caggggcatc gccacgctcc agcatggctt
     5941 aggcaaagtg tgctttgttc ataccggatg cggcgtgaac gccttatccg gcttacaaat
     6001 cgcgcaaatt caatagattg cagaggtaat gtaggtctga taagcgtagc acatcaggca
     6061 gctttgcgtt tgtcatcagt ctctgattag tactcgatcc atggatcgcg cttgctttca
     6121 ggcaagggtt gctctaatgc ctgcatttgc tctgccgaaa gtccagtata tcgttgtatc
     6181 cattcaggat ccgcaccgtt ctgcaaaagc aatctaagga ggcgttgttc cccttcttct
     6241 ttcccctttt ccatccctaa cagccatcca tcattatgaa ttcgctctgc aatcgtcatt
     6301 attcgctccc tttgttgtgg cattcgacgg gtaagttcac tgataaactc attaaaacgc
     6361 gcttcatcgc cagtcagtaa aatgtaattt aacagcgcag ttatctggct gtcattagca
     6421 cactctgtaa ccagtaatat taccagttga tcgataagcc ccatcagatc gcgctggcga
     6481 atatgctttt ggatcaactc caacagggcg actctgcgat gctgcacaat ctcgtcgtct
     6541 ggcacgacag tgacatccac cagcgggaac gctgcggtat aaagcttccg tgcggtggtc
     6601 gggtcagcaa attcgtccag ccagcacagg gaccagggat aaggactacg gctaccgtga
     6661 taaaacagca tcgggatgac cagcggtagc ggtcggcgtt tatcatgctc gatatggcgc
     6721 tgcatcaccg ccatggaata tcgcatcagg cgaaaggcca tatggatatc ctcgcggctc
     6781 tgatgttcaa tcactacgta aatataacca tcaccttcac gggtctttac cgaccacaga
     6841 atatcggagt gtagcgcccg caatttttca tcgacgaagc tggcggattc cagttttaag
     6901 ctgtcgagat cgcacagttc acgtaaatct ttgggtaagt ggatctccat aaaatcccgc
     6961 gcggtgtcag ggtgcgtgag aaaggattta aataatgcgt catgcggcgt actggtcgtg
     7021 aagtttgtca tggcgatccg tcacctgttg ataagatggc ccgactttag cgactaacaa
     7081 aaccattctc acccggtaat ttcccattcc ccgaggggct ttccaggcta tttttattct
     7141 tctgcaaacg atacattttt ccggaaacag gctcgcattt tacatttcct gtacccggca
     7201 gatattcccc tttccaccgc actcttttga ttaaccgcaa attttaatta cacttaaggt
     7261 gtatattttc tatgcaaccc atcaattcaa gaggtgtaat gtgctgatga ctatttgtaa
     7321 tcgttataca ttctgacctg gagtcagaag gtatttctct gtctgtgtat tcacaggcag
     7381 tgtggttgat tacatgaaat tcagtacatt tatccttctc gcctgccttt cgtcattacc
     7441 gacggtattg aatttcgttt tccccattgg ggttctccgg acaaggagtt gtttgttatg
     7501 ccacgttttt ttgcccgcca tgccgccacg ctgtttttcc cgatggcgtt gattttgtat
     7561 gactttgctg cgtatctgtc gacggacctg atccagccag ggatcattaa tgtggtccgt
     7621 gattttaatg ccgatgtcag tctggcccct gctgccgtca gtctctatct tgctggcggt
     7681 atggcgttac agtggctgct ggggccgctt tccgacagaa ttggccgcag gccggtgctg
     7741 attaccgggg cgttaatttt tacccttgcc tgcgccgcga caatgttcac cacgtcaatg
     7801 acacaatttc ttatcgcgcg tgcaattcag ggcaccagta tctgttttat tgccaccgtt
     7861 ggttatgtca cggtgcagga ggcgtttgga cagacaaaag ggatcaagtt gatggcgatt
     7921 atcacctcca tcgtactgat tgcgccgatt atcggcccgc tttccggcgc agctctgatg
     7981 cactttgtgc actggaaagt cctttttgcc atcattgcgg ttatgggttt tatctcattt
     8041 gttggcttac tgctggcgat gccagagacg gtgaagcgcg gcgcggttcc atttagcgcc
     8101 aaaagcgtct tgcgcgattt tcgtaatgtc ttttgcaatc ggctgttcct ctttggcgca
     8161 gcaaccatct ctttaagcta tatcccgatg atgagttggg tggctgtctc gccggtgatc
     8221 ctgattgacg caggcggctt aacgacctcg cagttcgcct gaacacaggt tccggtgttc
     8281 ggcgcggtga ttgtcgcgaa tgccatcgtg gcgcgtttcg ttaaagatcc gaccgaaccg
     8341 cgctttatct ggcgcgccgt accccttcaa ctggttggcc tcgcgctgtt gattatcggc
     8401 aatctgctgt caccgcacgt ctggctgtgg tcggtgctgg gcaccagtct gtatgctttc
     8461 gggattggtt tgattttccc gaccttattc cgctttacgc tgttttccaa taacttaccg
     8521 aaagggaccg tctccgcatc gctgaatatg gtgatcctga tggtgatgtc tgtctcggtc
     8581 gaaatcggcc ggtggctgtg gtttaacggc ggtcgcttgc cgtttcatct gttagccgtc
     8641 gtggcgggcg ttatcgtcgt tttcaccctg gcgggattgc tcaatcgcgt gcgccaacat
     8701 caggctgccg agctggcgga ggagcggtga tttttgcgcg atcccgccgt caggctctat
     8761 tcttaacatt atgaataaac tcattgaact cagacgcgcc aaaatgttgg cgctctcttt
     8821 actgcttatc gccgctgcta cctttgtcgt tacgctgttt ttgccgccca atttttgggt
     8881 gagcggcgtg aaggcgattg ctgaagcggc gatggtcggc gcgctggcgg actggtttgc
     8941 ggtggtggcg ctgtttcgcc gcgtgccgat tccgatcatt tctcgtcata cggcgattat
     9001 cccgcgtaat aaagaccgga ttggcgaaaa tctcggtcag ttcgtgcagg aaaaatttct
     9061 cgatacccag tcgctggtgg cattgattcg acgtcacgaa ccggcgttgt tgattggcaa
     9121 ctggtttagt cagccggaga acgcccgccg cgttggtcag catctgttgc agatcatgag
     9181 cggttttctt gaactgaccg atgatgcgcg tattcagcgc ctgcttaagc gcgcggtcca
     9241 ccgggcgatt gataaggttg atctttctgg caccagtgcg ttgatgctgg agagtatgac
     9301 caaaaacgat cgtcatcagg tgctgctcga tacgctgatc gcacagttga tcgcccttct
     9361 ccagcgcgat aaatcgcgca agtttatcgc ccagcaaatt gttcgctggc tggagagcga
     9421 gcatccactg aaagccaaaa ttttgcccac tgaatggctg ggcgaacata gcgcggagtt
     9481 ggtttctgac gcggtgaatt ctttgcttga tgatatcagc cgcgatcgtg cgcatcagat
     9541 ccgccatgcg tttgatcgcg ccacctttgc cctgatcgac aagctgaaaa acgatcctga
     9601 aatggcagcg cgagccgatg ccgtaaaaag ctatctgaaa gaagacgaag cttttaaccg
     9661 ctatctcagt gaattgtggg gggatttacg ggaatggctg aaagtggata tcaacagcga
     9721 agattctcgt gtgaaagaac gtatcgcacg agcgggtcaa tggtttggcg aaacgttaat
     9781 tgccgatgat gccttgcggg cgtcgttaaa tggtcatctc gaacaagccg cgcaccgcgt
     9841 cgcgcctgag ttttccgcat tcctgacgcg ccacatcagc gatacggtaa aaagctggga
     9901 tgcgcaggat atgtcgcggc aaatcgagtt aaatatcggc aaagatctgc agtttatccg
     9961 tgtcaacggt acgctggttg gcggttgtat tggacttatt ttgtatttgc tgtcgcagct
    10021 cccggccttg ttccccctcg gcaatttata gaaatccatg aaaaactaag tggctaaccg
    10081 cgagagagat caaataaacc acactaaaag aatggtaata atgtggcctc ttttcattat
    10141 ctcccgtggt acggggaagg aaaatcatgt cacttgtcac cgatctaccc gccattttcg
    10201 atcagttctc tgaagctcgc cagaaaggct ttctcaccgt catggatctc aaggagcgcg
    10261 gcattccgct ggttggcacc tattgcacct ttatgccgca agagatcccg atggcagccg
    10321 gtgcggttgt ggtttcgcta tgttccacct ctgatgaaac cattgaagaa gccgagaaag
    10381 atctgccgcg caacctctgc ccgctgatta aaagcagcta cggcttcggc aaaaccgata
    10441 aatgccccta cttctacttt tctgatctgg tggtcggtga aaccacctgc gacggcaaaa
    10501 agaaaatgta tgaatacatg gcggagttta agcccgttca tgtgatgcaa ctgccaaaca
    10561 gcgttaagga cgatgcctcg cgtgcattat ggaaagccga gatgctgcgc ttgcaaaaaa
    10621 cggtagaaga acgttttggg cacgagatta gcgaagatgc tctgcgcgat gccattgcgc
    10681 tgaaaaaccg cgaacgtcgc gcactggcca acttttatca tttggggcag ttaaatcctc
    10741 cggcgcttag cggcagcgac attctgaaag tagtctacgg cgcaaccttc cggttcgata
    10801 aagaggcgtt gatcaatgaa ctggatgcaa tgaccgcccg cgttcgtcag cagtgggaag
    10861 aaggccagcg actggacccg cgcccacgta ttttaatcac cggctgccca attggcggcg
    10921 cagcagagaa agtggtgcgc gcgattgaag agaatggcgg ctgggttgtc ggttatgaaa
    10981 actgcaccgg ggcgaaagcc accgagcaat gcgtggcgga aacgggtgat gtgtacgacg
    11041 cgctggcgga taaatatctg gcgattggct gctcctgtgt ttcgccgaac gatcagcgcc
    11101 tgcaaatgct cagccagatg gttgaagaat atcaggtcga tggcgtagtt gatgtgattt
    11161 tgcaggcgtg ccatacctac gcggtggaat cgctggcgat taaacgtcat gtgcgccagc
    11221 agcacaacat tccttatatc gctattgaaa cagactactc cacctcggat gtcgggcagc
    11281 tcagcacccg tgtcgcggcc tttattgaga tgctgtaagg agtggcagtg acatattcga
    11341 ttggcattga ttccggctca accgccacca aagggatctt actggctgac ggcgtgatta
    11401 cgcgccgttt cctcgttcca acatcctttc gcccggcaac agcaattact gaagcctggg
    11461 aaactctgcg cgaagggtta gagacaacgc cgtttctgac gctcaccggc tacggacggc
    11521 aactggtgga ttttgccgat aaacaggtga cggaaatctc ctgtcacggg ctgggcgcac
    11581 ggtttcttgc gccagcaacg cgcgcagtaa tcgacatcgg tggtcaggac agcaaagtga
    11641 ttcagcttga tgatgacggt aacctgtgcg atttcctgat gaatgacaaa tgcgcggcgg
    11701 gcaccgggcg tttcctggag gtgatctcgc gcacgcttgg caccagcgtc gaacagctcg
    11761 acagcattac cgaaaatgtc acgccgcacg ccatcacgag tatgtgcaca gtgtttgctg
    11821 aatcagaagt gatcagcctg cgctctgcgg gcgtagcgcc agaagcgatt ctcgccggag
    11881 tgattaacgc gatggcgcgg cggagtgcca atttcattgc tcgtctctcc tgtgaagcgc
    11941 cgattctgtt tactggtggc gttagccact gtcaaaggtt tacccacatg ctggaaagcc
    12001 atctgggaat gccggtacaa actcaccctg atgcgcaatt cgctggtgca attggcgcgg
    12061 cggtgatcgg tcagcggcag agaaaacgcg catgaaagag catctgtttt tatttcactc
    12121 cacggtcggc gtcatacaaa cccgcaaggc gttacaggca gcgggcatga cttttcgcgt
    12181 cagcgatatt ccccgtgatt tacgcggcgg ctgcgggtta tgcatttggc tgacctgtcc
    12241 gcctggcgag gaaattcaat gggtgatccc tgggcatacg gaatcagttt attgtcagca
    12301 ggatggtggc tggcgctgca ttgcacatta cgggatttct ccgcgttaag gattcattaa
    12361 gatcgctacg tatacttctc gatatcttat tgatttgcct taaagggatt accgtgacaa
    12421 aaagtatttc attaagtaaa cgcatcttcg ttattgtgat tctttttgtc attgtggcag
    12481 tgtgtacttt cttcgtgcaa tcttgcgccc gtaaaagcaa tcatgcagca tcatttcaga
    12541 attaccacgc caccattgac ggcaaagaaa ttgcaggcat aacgaataat atctcttcgc
    12601 tgacctggtc ggcacaaagc aatactcttt ttagcactat caacaaacct gccgccattg
    12661 ttgaaatgac caccaatggt gatttgatcc gcacaattcc tctggatttt gttaaggatc
    12721 tcgagacgat cgaatacatt ggcgataatc agtttgtcat tagcgatgag cgcgattacg
    12781 ccatttatgt gatttcactg accccaaact cggaagtaaa aatcctcaaa aaaataaaaa
    12841 tcccgctgca ggaatcccca accaattgcg gttttgaagg attggcctat tctcgccagg
    12901 atcacacttt ctggtttttt aaagagaaaa acccgattga ggtctacaaa gttaacgggc
    12961 tattaagtag caatgaattg cacatcagca aagacaaggc actacaacgg caatttactt
    13021 tagatgatgt gtccggcgca gaatttaacc aacaaaaaaa tacgcttctg gtgctgtctc
    13081 atgaatctcg cgcactccag gaagtaacgc tggttggcga ggttatcggt gaaatgtcac
    13141 taaccaaagg cagtcgaggt ttgtcgcata acatcaaaca ggccgaaggg gttgcgatgg
    13201 atgcctctgg taatatttat atcgttagcg aaccaaatcg tttttatcgc tttacccctc
    13261 aatcatcgca ttaacaggct ggcatgcttc acctttttgt tattaacttg ttaacaacaa
    13321 aaatgacaaa caaggttaaa catgccttcg tccacgcatc ccgtagaacg cttttctttc
    13381 agcaccgcgc ttttcgggat gctggttctg accttaggta tgggtttagg ccgcttcctt
    13441 tatacgccta tgttgcccgt catgatggcg gaaggatcgt tttcatttag ccagctctcg
    13501 tggattgcca gcggcaacta tgcggggtat ctggctggca gtctgctatt ttcttttggc
    13561 gcatttcacc agccatcgcg cctgcgccca tttctgttgg cttctgccct ggcgagcggg
    13621 ttgttgatcc tcgcaatggc atggttgccg ccatttattc tggtgttatt gattcgcgtc
    13681 ctggcgggtg tcgccagcgc cggaatgctg atttttggtt cgacgctgat tatgcagcac
    13741 acgcgccatc cttttgtgct ggcagctttg ttttctggcg ttggcattgg catcgcactg
    13801 ggcaatgaat atgttctggc aggcctgcat tttgacctct cctcgcaaac gttatggcaa
    13861 ggcgccgggg cgctttctgg catgatactg attgcactta cgcttttaat gccctcgaaa
    13921 aaacacgcca tcgtaccaat gccattggca aaaacggagc aacagataat gagctggtgg
    13981 ttactggcta ttctgtacgg cctggcggga tttggttata tcatcgtcgc gacttacctg
    14041 ccgctcatgg cgaaagatgc aggctcacca ttgttaaccg cccatctctg gacgttagtc
    14101 ggcttatcaa ttgtgcctgg ttgctttggc tggctatggg ccgcaaaacg ttggggggct
    14161 ttgccctgcc tgacggcgaa tttgctggtg caggctatct gtgtgctgct tactctcgcc
    14221 agcgactcgc ctctcttgct tatcatcagc agtcttggtt ttggcggtac ctttatgggc
    14281 acgacatctc tggtgatgac tatcgcccgc caactgagcg taccgggaaa tctcaatctt
    14341 ttaggctttg tgacgctcat ttacggcatt gggcaaatcc tcggcccggc gctgaccagt
    14401 atgctcggca atggaacatc ggcgcttgcc agcgccactc tctgcggcgc ggcggcgttg
    14461 tttattgctg cgcttatcag cacagtgcaa ttatttaagc tacaagtggt cacttcgtga
    14521 gagaagtttc ccttaccact caagaaactc aaccgcaact gttgatgtca gccagacccc
    14581 gttttccgct tgccaaaaag ggatgcctcg tttcgccatt tcctgtgctt tgacggttaa
    14641 aataactggc gatccgtgtc ttgctcccac tttgcgtgcc gttgcttcat cagcagagag
    14701 atgcacgtaa tggcgctccc ctgcaatcag cccttgtttt tttatctcgt caagaaaacg
    14761 gcttgccgta ccatggtaga gaaattgtgg cggtgttttt tcgatgaagc caatggcgac
    14821 ctgcgaagtc gaatgcccct gaacggcgcg aatacacgta ccatcactgg aataactaaa
    14881 gcgcttttta tcgctggtag cgacaacgat atccagtaat gcgcgggtta accgcttgcc
    14941 cgctttttgg gcacagagta tgagcttatc gatatccgcc catccttcac ggtccagcac
    15001 gatgccaatc gcctctggtt tatgtcggag gacaaaactt aaaaacttgc tcgtctctgc
    15061 cagctctttg tcgttgtatt ttgccatctc gttctcgtcc ctggattttc tgtttttatt
    15121 tactgatgtc attgtcactg ggtttatgaa ttttctgcaa ctcaatcctg cgcagaacct
    15181 ttgccacaca gcgtcatttt tccgctatgc aaaaaacgca tagccagaaa catacttgca
    15241 ttttccccat acccacatgg ctgataaggt ttttcagctc caggaatttt cctggctcaa
    15301 gcaacacaca aacacaaaaa aacaatgggg attgttatga cacaacaagg ggatgctgta
    15361 gccggggagc tggcgacaga aaaagtgggc attaaagggt atctggcttt ttttctcacc
    15421 ataatctttt tttccggtgt tttttccggg acagacagtt ggtggcgcgt ttttgatttt
    15481 tccgtgctga acggatcgtt tgggcaactg cctggtgcga atggcgccac cacctcgttt
    15541 cgcggcgcag gtggcacagg agctaaagat ggctttctct tcgcgctgga actggcaccg
    15601 tcagtcattc tctctctggg gattatttca attaccgatg gtcttggcgg attacgcgcc
    15661 gcgcagcagt taatgacgcc cgtgctaaaa ccgttgttgg gcattccagg catctgttca
    15721 ctggcgctga ttgctaacct gcaaaacacc gatgccgccg caggtatgac caaagaactg
    15781 gcgcaggaag gggaaattac cgaacgtgat aaagtgatat tcgccgccta tcaaaccagc
    15841 ggtagcgcca tcatcaccaa ctacttctct tccggcgtcg cggtgtttgc ctttctgggc
    15901 acgtccgtaa tcgtaccttt agccgtcatc ctggtgttta aatttgtcgg agccaacatt
    15961 ttgcgcgtct ggcttaactt cgaagaacgt cgcaacccaa cgcaaggagc gcaagcatga
    16021 caactcaggt ccgcaaaaac gtcatggata tgtttattga tggcgcgcgc cgtggcttta
    16081 ctatcgctac caccaacctg ctgccaaacg tggtgatggc ctttgtcatc atccaggcgc
    16141 tgaaaattac tggcctgctc gactgggtgg gacatatctg tgaacccgta atggcgctgt
    16201 ggggattgcc cggtgaagcg gcaaccgtcc ttttagccgc attgatgagc atgggcggcg
    16261 cagtcggtgt tgccgccagt ctggcaaccg ctggcgcatt aaccggccac gatgtcaccg
    16321 ttttactgcc cgcgatgtat ttaatgggca acccggtgca aaatgtcggt cgctgcctgg
    16381 gaacagccga agtgaatgcc aaatattacc cgcatatcat tacggtctgc gtgattaacg
    16441 cattgctgtc gatctgggtt atgcagctta ttgtttaaca aggagttacc atgattgatt
    16501 attccgcagc cggttttacc ctgctgcagg gagcgcattt gtatgcgccg gaagatcggg
    16561 gaatttgcga tgtcctcatc gctaacggca aaattattgc cgttgccagc aacatccctt
    16621 ctgacattgt accagactgc acggttgtcg atctcagcgg gcagatcctc tgcccaggtt
    16681 ttattgatca acacgtccat ttgattggtg gtggcggcga agcaggtccc acgacacgca
    16741 cgccggaagt ggcgttaagt cgtctgacgg aagcgggcgt cacgtcagtg gttggtctgc
    16801 tgggtaccga ctctatctct cgccacccgg aatccctgct cgccaagacc cgtgcgctca
    16861 atgaagaagg catcagcgcc tggatgctga ccggcgctta tcatgtccct tcccgcacca
    16921 ttacaggttc cgtggaaaaa gacgtggcga ttatcgatcg cgtgattggt gtgaaatgcg
    16981 ccatctctga tcaccgttct gccgcacctg acgtttatca cctggctaat atggcggcgg
    17041 aatcccgcgt tggcggtttg cttggcggta aacctggcgt caccgtgttc cacatgggcg
    17101 acagcaaaaa ggcgttacag cctgtctatg acctgctgga aaactgcgat gtgccgatca
    17161 gcaagctgct gccgacccac gttaaccgca acgttccgtt gtttgagcag gcgctggagt
    17221 tcgcgcgcaa aggcggcacc atcgacatca ccagtagcat tgacgaaccg gtcgcccctg
    17281 ccgaaggtat tgcccgcgcc gttcaggcgg gtattccgct ggcacgcgtc accctcagct
    17341 ccgacggcaa cggtagccag ccgttctttg atgacgaagg aaatttaacc catatcggtg
    17401 ttgcaggttt tgaaacattg ctggaaaccg tgcaaatgtt ggtcaaagac tatagcttca
    17461 gccttagcga tgccctgcgc ccgctcacca gtagcgtagc cggtttcctt aacctgaccg
    17521 ggaaaggcga aattctgcca ggcaatgacg cagacttact ggtcatgacg cctgaattgc
    17581 gcattgagca ggtatacgct cgcggcaaac tgatggtcaa agacggcaaa gcctgcgtga
    17641 aaggaacgtt tgaaacggca taaccggttg tcagccgccc gaatggacgt agaatgcccg
    17701 caagggcggc tgacagagta taacgtaatg gatgactgtg gtgcggtttt gcataacatt
    17761 gaaaccaaat ggctttatga ttttctgacc ctggaaaaat gtcgcaattt ttcccaggcg
    17821 gcagtcagtc gcaacgtctc gcaaccggca ttcagccgcc gcatccgtgc actggaacag
    17881 gcgattggcg ttgaattgtt taaccgccag gtgacgccgc tgcaactctc ggaacaaggg
    17941 aaaatcttcc attcgcagat ccgccatctg ttgcaacagt tagaaagcaa cctggcagag
    18001 ctgcgtggcg gcagcgatta cgcgcaacgt aaaatcaaga tagccgctgc acactctctt
    18061 tccctcggtc tgttaccgtc cattatcagc cagatgccgc cgctctttac ctgggcaatt
    18121 gaagccattg atgtcgatga agcggtcgat aaactgcgcg aagggcaaag tgactgtatt
    18181 ttttcctttc acgacgaaga tttgctggaa gcgccgtttg accacattcg cttatttgaa
    18241 tctcaattat tccccgtctg cgccagtgac gaacacggag aagcactgtt taacctcgcg
    18301 cagccacact ttccgttact gaattacagc cgcaactcct acatggggcg attgattaat
    18361 cgcaccctga cgcgccacag tgagttgagt ttcagcacct tttttgtctc ttcgatgagc
    18421 gagctattaa agcaggttgc cctcgacggc tgtgggattg cctggctgcc ggaatacgct
    18481 atccaacaag aaattcgcag cgggcagctc gttgtactga atcgggaaga actggtgatc
    18541 ccgattcagg cctacgcata ccggatgaat acccgcatga atccaattgc cgaacgcttc
    18601 tggcgtgaac tgcgcgagct ggagattgaa cctggttgaa agttttctgc agaaaatctt
    18661 ccggcgctgg gaattatgat tgaggttcct gccgcagtca tgattgcaga caaattagcc
    18721 agtgaagtcg atttcttttc catcggcacc aacgatatga cgcaatatat tatggcggca
    18781 gacagaggaa atagcaccgt cgcgaaactg gtcgattatt gtaatgacgc ggtgattaac
    18841 gctattgcta tggtgtgtca ggccggacgt aataatgaaa ttccggtcag tatgtgcggc
    18901 gaaatggctg gagatatcca gcaaacagca cgactgctca ccatggggat tgataagctc
    18961 agtgccagtc catcccggct tccggccttg aaagcagcaa tcagagcctc ccactaattg
    19021 tgacctaacg cgatacgtct tgcgcgtatc gcgtttcccg tcatttactg cggcatcgtg
    19081 cgatgccagt tccccttctg ccagaccagg tcaaacgtta gcggctcatc atcgtggtac
    19141 agcatcatct ccagactaat ggccaactcg tggcaatagg cttctttcag attgacctgc
    19201 aatgccgcaa tacgtggctc atgaaataaa agcgcggaac gtaactggcg gcaataccaa
    19261 ctctggagaa gcttgtcgct ctgattatgc ccttgccact caggcatgcc tgaaacaagt
    19321 aatattggta gcggtgaatg gggagaacta aaaagttgaa agagatcccg gcatacggaa
    19381 tcacgcagcg ataacgtatt gtgtcctgta ctttcaggta ataccgtctg aactgattgg
    19441 cgcatcattt tgcgcactcc tgacgtttag tggcatctgt atcctgccct ggctgccgga
    19501 caccactggg ttgatgctca tattccataa tcattccttt atgctcattg cgtccctggc
    19561 actttcataa gggatttata agatccgcca aagataaaac actgtacaga aaagaatatc
    19621 cactg
//
LOCUS       DAZUCC010000081        19603 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZUCC010000081 DAZUCC010000000
VERSION     DAZUCC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.081"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            233..733
                     /gene="tssB"
                     /locus_tag="ACHY09_002492"
     CDS             233..733
                     /gene="tssB"
                     /locus_tag="ACHY09_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFS3885450.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            768..974
                     /locus_tag="ACHY09_002493"
     CDS             768..974
                     /locus_tag="ACHY09_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885451.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPY"
     gene            1043..2518
                     /gene="tssC"
                     /locus_tag="ACHY09_002494"
     CDS             1043..2518
                     /gene="tssC"
                     /locus_tag="ACHY09_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFS3885452.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2525..2938
                     /gene="tssE"
                     /locus_tag="ACHY09_002495"
     CDS             2525..2938
                     /gene="tssE"
                     /locus_tag="ACHY09_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFS3885453.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2942..4792
                     /gene="tssF"
                     /locus_tag="ACHY09_002496"
     CDS             2942..4792
                     /gene="tssF"
                     /locus_tag="ACHY09_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS3885454.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     LRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSHPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIHS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4756..5838
                     /gene="tssG"
                     /locus_tag="ACHY09_002497"
     CDS             4756..5838
                     /gene="tssG"
                     /locus_tag="ACHY09_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFS3885455.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5863..7143
                     /gene="tagH"
                     /locus_tag="ACHY09_002498"
     CDS             5863..7143
                     /gene="tagH"
                     /locus_tag="ACHY09_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFS3885456.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCVFTQQGNVGHGPECQWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITAGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7140..7664
                     /gene="tssJ"
                     /locus_tag="ACHY09_002499"
     CDS             7140..7664
                     /gene="tssJ"
                     /locus_tag="ACHY09_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFS3885457.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7667..8998
                     /gene="tssK"
                     /locus_tag="ACHY09_002500"
     CDS             7667..8998
                     /gene="tssK"
                     /locus_tag="ACHY09_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3885458.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGILPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWAEIAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9003..9764
                     /gene="icmH"
                     /locus_tag="ACHY09_002501"
     CDS             9003..9764
                     /gene="icmH"
                     /locus_tag="ACHY09_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFS3885459.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FYGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9773..12556
                     /gene="tssH"
                     /locus_tag="ACHY09_002502"
                     /gene_synonym="clpV"
     CDS             9773..12556
                     /gene="tssH"
                     /locus_tag="ACHY09_002502"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFS3885460.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEINLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQALVQEIIALRQQLLGVAEDD
                     AAPLPDADTVEDTPPESESESESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQSFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEELYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDDAFTADVEDA
                     QNDESVTKDETVL"
     gene            12553..13296
                     /gene="vasI"
                     /locus_tag="ACHY09_002503"
     CDS             12553..13296
                     /gene="vasI"
                     /locus_tag="ACHY09_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFS3885461.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTP
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            13301..14713
                     /gene="tssA"
                     /locus_tag="ACHY09_002504"
     CDS             13301..14713
                     /gene="tssA"
                     /locus_tag="ACHY09_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFS3885462.1"
                     /translation="MAMNLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQHDALSGELAKLAQCWQSYN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAT
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14822..18256
                     /gene="tssM"
                     /locus_tag="ACHY09_002505"
     CDS             14822..18256
                     /gene="tssM"
                     /locus_tag="ACHY09_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFS3885463.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGIYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     GLFPQALLAEPNLATESRTWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPLGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKL
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18267..>19603
                     /locus_tag="ACHY09_002506"
     CDS             18267..>19603
                     /locus_tag="ACHY09_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFS3885464.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTDNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQPKSNAENAAQTTVRTLIYAPETMASSTPEAVVPPLPDLPEMK
                     VEVRSLSENPPQASVMKQGSTVRGFIAGIVCSVAIASALWWWQVYPAQQQLLQVNDTA
                     QGAATVWMASPELENYERRLHQLLGVSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPLESLLTQYQDARAQKQNTDALEKQINERMDGVLSRWLLLKNNTIPTIKKD"
BASE COUNT         4552 a         5430 c         5473 g         4148 t
ORIGIN      
        1 cacctgacgc gaattgcaca ggttcacaca atctatttaa cgcttgcaga atacccctct
       61 ttacgatcga taagaagtcc ttttcttatt tattttgaag tgtagaatgc ggggtgatga
      121 aggagtggta attttatttt caccgccatt agggcggcat tgccgatatt tttttgcggt
      181 acaaatatgc cgtgttcatg gcctgtacga gataacatga cggatcactt ttatgagcaa
      241 aaaatttgaa ggcagcgtcg cgcctcgcga gcgtataaat atatcctatg tcccgaagac
      301 ggacggccag acggctgaag tagagcttcc gcttaacatg ctcgttgtcg gcgataccgg
      361 aaatacgcag gagacatccc cactggatga acgtcaggca gtttccgtta acaagcacaa
      421 tttcggtgca gtaatggctg aagcagccat tggcctcaat tttaccgtcc ccgccacgct
      481 gaagggaagc acaacggacg atgaactgaa tgtggcgctg aatatcaagt cactggatga
      541 tttttcaccg gacagcgtgg cccggcaggt tccggaagtg aataaattgc tggaactgcg
      601 tgaagcactg actgcgctga aggggccgat gggcaacctt ccggcattcc gtacccagct
      661 ccaggcattg ctggaaaatg aggagtcccg cgagcagttg cttaaagaaa tcggccaggt
      721 gagtgggaaa taaccaccac tatttcatta aacagggtta tccagacatg gcggcacaca
      781 aaaatatctc cgggaaacac cccctctctg acgaagacat tttgcaggcg gtttaccggg
      841 tatgtttcag cccgtccggt gaaatcaacc gggatggcgt ggatgcgctg attacaacac
      901 tggatcgccg tatcagtgat ctattggatg agattatcca tctttgtgag tttcagcaga
      961 gcgcgccgta ctgacggcgc gtacttcact gacggcgaaa acccggtttc agaaacacag
     1021 ctaaaacaga aggattttca gtatgtcttt acaggaagag gaactcgttt ccagtcatgc
     1081 cgggcagact gatcaggcgt cgtcgctgct tgatcaaatc atggcacaga cccgtattca
     1141 gcctggttcg gaaggttatg acgttgcccg ccagggtgtg acagccttta ttgccagcat
     1201 cctgcaatcc actgcatctg ccgaaccggt caataaactg gccgtggaca gcatgattgc
     1261 agatatagat gaacgcatca gccgccagat ggatgtcatc atccatgcgc ctgcctttca
     1321 gcaggtcgag tcgttctggc gctcactgaa aaccatggtg gatcgcgttg atttccgtga
     1381 aaacatcaag gtcaacgtgc ttcatgtcac gaagcaggaa cttctggaag actttgaatt
     1441 tgccccagaa attattcagt ccggatttta taaacacgtt tattcgtccg gattcggaca
     1501 gtttggtggt gaacccatcg ctgccgtgct gggcgcttat gagttcaaaa ataccgctcc
     1561 tgacatgaaa ctgctgcaat acgtcagtgc cgtgggcgca atggcgcacg caccgttcct
     1621 gtcctccgtt tccccggagt ttatggggct gaactcgtgg accgaactgc ctaatatcaa
     1681 agatctttat gccatctttg aaggtccggc ctacaccaaa tggcgcgccc tgcgtgactc
     1741 ggaagattcg cgctatttag ggcttacggc accccgcttc ctgcttcgcc agccctattc
     1801 accaacagat aaccctgtta agaactttaa ctattacgaa gatgtcagcc agaaccacga
     1861 agattatctg tggggtaata cggcctggat gctggcatgc aatatcgcag acagttttgc
     1921 caaataccgc tggtgtccaa acattatcgg tccgcaaagc ggcggcgcag tgaaagatct
     1981 gccggtgcat ctgttcgaaa cgatggggca gattcaggcc aaaatcccaa ccgaggtgct
     2041 ggtcaccgat cgccgcgaat ttgaactggc tgaagagggt tttatcaccc ttaccatgcg
     2101 taaagactct gataatgcag cctttttctc tgcaaactcg gtacaaaaac cgaagcactt
     2161 tccgggaaaa gacgcagaaa ccaactataa gctgggtacg cagcttcctt atctcttcat
     2221 catcaatcgg ttagcgcact acatcaaggt gttgcagcgt gaacaactgg ggtcatggaa
     2281 agaaaggagc gacttagaaa gagagcttaa tacctggatc cgtcagtatg ttgccgacca
     2341 ggaaaacccg cctgcagacg tgcgcagccg caaacctctg cgcgctgcca aagttgaggt
     2401 tatggatgta gaaggcgaac ccggctggta ccaggttgcg ctaagcgtga ggcctcattt
     2461 taaatttatg ggggcaaatt ttgagctttc cctggttggc cggttagaca gggagtaaca
     2521 caccatgata ccggatcggg aaagaaccac aggttctctt tttgagcgta tggaagcctc
     2581 ttcagccaga aaccggcagg gaggcagtat tcattcgctc aggcaatcca ttcgccagaa
     2641 cctacgaaac attcttaata cccggtccgg tagttgccgg ggtgcgcctg agctgggcat
     2701 tgatgaaccg gaaggggctg aaaacttccg cgaatcaatg agtcgggcca tcgagcaatg
     2761 tatagaacgc tatgaacccc ggatctcaca cgctgaagtg caggcagtgg tttcatccgc
     2821 ctccagcccg ctggatatga cgtttcatat tacagcctgg gtgacattta acgaaacgca
     2881 tgaggtgctt gaatttgata tggcaccgaa tggcagccag cattaccggg tggactgaca
     2941 aatggaattt gaagaacgct atttccggga agaactcgat tacctgcgcc agcttagcaa
     3001 gctgctggca acggaaaaac cccatctggc ccgcttcctg gccgaaaaag atgcagatcc
     3061 ggatattgaa cgcctgctgg aaggggtggc ttttcttacc ggcaatctcc gccagaaaat
     3121 tgaggatgaa ttcccagaac tgacgcacgg gcttattaag atgctctggc ctaattacct
     3181 gcgtccggtt ccggcaatga cccttattga atatacgccg gatatggata agtcttctgt
     3241 accggtgtta atcccccgta atgagcagtt tacaaccaac gccggggaaa tcagagttga
     3301 tgaagtgctg ccctctgatg ctaaaaagga ggagcctcct ccctgtacct tcaccctctg
     3361 ccgggatatc tggctgctgc ccgttcgcct ggagcagatt gaaaaccgca gtacgacccg
     3421 taatggtgtt atcaacatca ccttttcggt cgcaccggga acggacttcc gcacgctgga
     3481 tctgaacaaa cttcgcttct ggctcggcaa tgacgacaac tatacccgtg accagcttta
     3541 tttatggttc tgcgaatact tgcagggtgc tgacctgact gtgggtgaac agcatcttcg
     3601 cctgcctgag tttatgctaa aagctgtcgg ttttgagccg caggatgcca tgctgccctg
     3661 gccgaaaaac gtccacagcg gctaccggat ccttcaggag tatttctgtt accccgatgc
     3721 gtttctcttt tttgatcttt gtggttgtcc ggctttgcct gacggattgc aggcggaatt
     3781 ctttaccctg caactgcgtt tttcgcaccc attgcccgtg gacatccggt tgcgccgcga
     3841 ctccctgcgc ctgtattgcg cgcctgccat taatttattt atccaccatg cagaagccat
     3901 cacgctggac aaccggcggg cagactatcc gctggttccc agccgccatt atccggaaca
     3961 ttacgatgtc ttttccgtta acggcgtgat aagccaggtg caggatatgt tcaggaaaaa
     4021 agatctgggt cgtcctgttt cgacgcaggc cgcgcgccag tggcctgcct ttgaaagttt
     4081 cagccatcag atggaataca gccggaagcg ggaagtggtg tactggcatc accggaccaa
     4141 aacatccctg ttccatcgcg gctttgatca tacccttgct tttatacatg ctgatggcag
     4201 ttatccgtca gacgaatcgc tgctcagtaa tgaagtggtt tcggtatcgc tgacctgtac
     4261 caaccgtgaa cttccgtcac aaattcattc cggcgatatc accggcacaa ccggtaaaaa
     4321 tgcggctgtt gcttcatttc gcaacattac ccgcccgacg caaccgctct ggccggtcat
     4381 tgatggcagc ctgcactggt ccctgctctc cgccatgaac ctgaattatc tgtcattact
     4441 ggatacggac gcgctgaagc aggtcatcgc caactttgat cgccacgcaa tccatcatcc
     4501 gcagacggcg cggctgtcac aacaaaagct ggatgccatt gagcgtctgg agacccgccc
     4561 tgtcgatcgc ctgtttacgg gcattcccgt ccgggggctg gcctccacgc tgtatctgca
     4621 cccggagccg tttgtctgtg aaggggaaat gtatctgctc ggtacggtgc tttcgcattt
     4681 tctgtcgctg tacgccagcg ttaactcatt ccacatgctg accgttgtga acacagaaag
     4741 ccaggagaca tggaaatgga cggaaagaac cgggcagcat cctcttatct gagtccggga
     4801 aatcccccgg cagataaaga gcagaatgat ccgctggcac aggtttttca taacgcctgc
     4861 tcttacaatt tttttgcgat ggcggagctg ttgcaccgcc tggcaaaggg tgaaaagggg
     4921 acgccagaat tatccctgcg tgacgatccg gcacaggaaa ccctgcgttt cagcgccgat
     4981 gccagccttg ccttcccgtg cagtgatatc agtgcgctga aacgggatac gtcaggggca
     5041 ttcaggatga ccaccacctt tatggggtta caggggagcc agtcgcctct ccccggttat
     5101 tacctcgatc acctggcctg gaaagcggta catgaacaaa gtccggtagg tgattttctg
     5161 gatatgttca gccaccgcct gacccagttt gtctggcata tctggcgtaa gtaccgctat
     5221 cacatcagtt ttcgtaacgg cggtgtggat gcgttctcgc agcgcatgta ctcccttgtc
     5281 gggctgggcc accgccagct tcgcgataag ctggcgatta atcacagcaa aatgctggcg
     5341 tattccggga tcctggcaaa tccgggacga tccccggaaa taatctgcgg cctggtgtcc
     5401 cactgctttg atttgtcaga cgtgacgctg caaaactggc aacgtcgcaa ggtggatatt
     5461 gaaccggatc agcaaaacag tctggggagc tattcactta aaaatggcga aaaactggcg
     5521 ggaagatcgg tactgggcaa ctttgttctg ggtacacggg tgcccgatct cagcggaaaa
     5581 ttccagctca gtattaccag tctgacccga aaacagttcc tctcttttct gccgtccggc
     5641 gaaaacttcc tgccactgac gatgtttgtg tccttcatcc tgcgcgatca gcttgcctgg
     5701 gacttacatc tggggctggc gccggagcag gtgggcgcaa tgcgcctggg cgataacaaa
     5761 agtgcgctgc tgggctggac cagcttcctc ggcacaccgg aagaacgacc ctcagtcacc
     5821 atcagggtac ggtcgtaaat tatgataatc acggagcaga atatgccgga agagaaacta
     5881 cagacgcttt cattgcaggt cattaacggc agtgagctgg aaagcgggcg ggcggcgcgc
     5941 tgtgtgttca cacagcaggg aaatgtcggc catggccccg aatgccaatg gtcggtacag
     6001 gatcgtcagc agagcattcc ggctcaggct tttaccgtta tcctgcacga tggcacattt
     6061 tgtctgcgcc cgcagacggc acaactgtgg ctgaaccagg caaaagtcac agcaacatca
     6121 gacctgatac agctgcgcca gggcgatgag atccagatag ggcgtctgat ggtgagggtt
     6181 catctgaacc ggggagatat cccccattac gatgaggaaa tggccactcc cgaaaccatc
     6241 gtcaccaatc gcgatatgct cacggatacc ctgctgtcaa cggagggtgc gccacactat
     6301 ccgggaatga ctcaccggca tcagcttgca gacaccgtgg taaacggttt ttctaccgat
     6361 ccactccagg cacttcagtc cgaaagcctg attaccgcgg gcgatccgct ttcaggcatt
     6421 gcggctgtcc ggccatcggc accgctgtcc gatccggcaa gtaatggggg gatcaatact
     6481 ccgtttatgg atctgccgcc catttatgcc agccccggcg atcgtaatga tgacgtctct
     6541 gtggcagaaa tggcgcaacg ccaccttgcg gtcaccccct tactgcgcgg tctcggcggt
     6601 tcgcttaccg tgagcaattc cgacgatgcg gatgattttc tggaagaggc cggacgcacg
     6661 ttacaggccg caataaaagg tctgctcgat ttgcagcaac agcgtaacag cctctcagac
     6721 aaacatttgc gcccgctgga agataacccg ctgcgcctga acatggatta cgccaccgcg
     6781 ctcgacgtga tgtttgccga aggtaaaagt ccggtacatc tggcggctcc cgccgctgtc
     6841 agtgaaagcc tgcgcaatgt ccgccaccac gaagaagcta accgggcagc gattgtggag
     6901 tcacttcgtg tcctgcttga cgctttctca ccacaaaatc tgctgcgccg ctttgtgcag
     6961 taccgccgca gccatgaact gcgccagccg ctggatgatg ccggagcatg gcaaatgtac
     7021 agccattatt acgaagaact ggcctccgat cgccagcagg ggtttgagat gctgtttaac
     7081 gaggtctacg cccaggtcta tgaccgggtg cttcgtgaaa aacagcggga gccggaagca
     7141 tgaacaggaa ggcgtttctg gcctgcgtgc tgatgtgcgt catattaacc ggctgcgaaa
     7201 cggcgaaaaa aatcagcgag gtgatcaaaa acccggatat ccaggtcggg agcctgaagg
     7261 agcaaccttc ggaaataacc gtcaccctgc tgaccgaacc ggacaccaat accaatgcgg
     7321 agggtgaatc agccgccgtg gatgtgcaac tggtgtacct gactgacgac tcaaaattgc
     7381 tggcagccga ctatgaccag atagccagta cgccgctacc ggatgtgttg ggtaaaaact
     7441 atatcgacca ccaggatttc aacctgttgc cggacaccat caaaaccctg ccgccggtaa
     7501 agctggatga gaaaacgcag tttattggtg tcgtggccta tttctccgat gaccaggcca
     7561 ctgaatggaa acaaatcgag acggtggaag gaaccggcca ccattaccgc ctgctggtgc
     7621 atgtacgtca aagcagcatc gaaatgaaaa aagaggatga ataattatgg caaccacccg
     7681 caacaaggtg atgtggcagg aagggatgct gatgcgccca caccatttcc agcaacagca
     7741 gcgttacaac gactacctgg ataaccagcg tttccgggcc atgaatgatt tatcctgggg
     7801 atttaccgaa ctcaccctca acaatgaact gctggcgcag ggtaagatca tgattgacag
     7861 cgcgtcaggc atactgcccg acggcaccgt cttttctatc cccgaccagg acgcactgcc
     7921 cgatccgctg cacccgcaaa attttccgga cgaaagaagc cgcaatattt accttgctct
     7981 gccggtcgcc agtgatgtga gaaatgaaat cagcgacggg cggcgaatcg ggcgttaccg
     8041 gctgaattat gccgatgtcc gggatctgca ttcagaagaa ggtgacgcgc gaacactgac
     8101 gctgggtcaa ctgacgccgc gcattatgag cggtgcagaa gatatgagcg cctatattac
     8161 gctcccgctt tgccgtatca gcgatcgcca tgccgacggc tccctgacgc tggatgacga
     8221 ttttatcccc tcctgccaga atattcaggt cagtaagaaa ctgcgtgttt atctcaaaga
     8281 ggtacaaggg gccattggcg gacgggcaag cgatctggca aaccgcattg gctctccggc
     8341 gcagagcggc atcgcggatg tggcggaatt tatgatgttg cagttactta accgtaacca
     8401 gacccggttt acccatcgcg ctcgtcgatc ccagctccac ccggaagatt tctaccttga
     8461 tcttgccggg ttgctgggtg aactgatgac ctttaccgag ccgtcgcgcc tgccctgccc
     8521 gcttgatgtg tatgatcatc atgacctgac caaaacattt aaaacgctgt taccggaagt
     8581 caaacgggcg ctgcataccg tactgtcgcc aagagccgtc aatctgccgc tgcatctgcg
     8641 tgacggtatc tggcaggccg atattcatga cacagaactg ctgcaatctg ccacctttgt
     8701 gctggccgtg gcggcaaaca tgccggtcga tcagatccag cgccagttta tccagcagtc
     8761 gaaaatttcc tcgccggaaa aaatccgcaa tatggtcagt gtgcagatac ccggcattcc
     8821 attgcgtgcc ctgatggtgg ctccccgcca gcttccttac cattccgggt tcagctattt
     8881 cgaactcgac aaaagcggcc aggcctgggc tgaaatagct gccgccgggg ccgttgcact
     8941 gcatgtttcc ggcagtttcc cggatctgaa catgcaactg tgggcgataa gagggtaaga
     9001 cgatggatga ggggtcactg tctctgccac catttaccgg acacgacgaa aaaagccagc
     9061 gcgactacca tctcgcgctt cgtggcaaca gcctgaatcc gatgatcgac gcggccacgc
     9121 cgctactcgg tatggtaatg cgcctttcaa ccatgaacag ccagacgatg ccggagcatc
     9181 tttttgctca ggtggtgacg gatgttcagg ccgtggagca actgcttcag gagcagggct
     9241 acgaaccggg cgtgattatc tcgtttcgct acatcctctg cacgtttatt gatgaagcgg
     9301 cgctcggtaa cggctggtcg aacaagaacg agtggatcaa acagtcattg ctggtccatt
     9361 tccacaacga agcctggggt ggagagaaag ttttcatcct gctggagcgc ctgatccgtg
     9421 aacccaaacg ttaccaggat ctgctggaat ttctctggct ctgtttttct ctgggatttc
     9481 gcgggcgcta caaagtggca gcacaggatc agggggaatt tgagcagatc taccgccgcc
     9541 tgtaccacgt cctgcataaa ctacgcggcg atgccccttt cccgctgtta caccaggaca
     9601 aaaaaaccca gggcgggcgt tatcagctca tcagtcgcct gaccattaaa catatttttt
     9661 atggtggcgt tgtcgtgctg gcgttgtttt acctgttcta tctgttgcgg ctggacagcc
     9721 agacgcagga catcctgcac cagctaaaca aattacttcg ataaggacgt ttatgatcca
     9781 gattgatctt cccacgctgg taaaacggct taacctgttc tcccgccagg cgctggagat
     9841 ggccgcctct gaatgtatga gccagcaggc agcggaaatt accgtcagcc atgtgcttat
     9901 tcagatgctc gccatgccac gcagtgacct gcgggttatt acccgccagg gcgatattgg
     9961 catggaagag ttgcgccagg cgctgacggt ggagaactac acaactgccc gttctgcgga
    10021 cagctacccg gcgttttccc cgatgctggt tgagtggctg aaagagggct ggctgctggc
    10081 gtcggctgag atgcagcaca gcgaattgcg tggcggcgtg ttactgctgg ccctgctgca
    10141 ttcgccgctg cgttatatac cgcctgctgc cgcccggctg ttgaccggca ttaaccgtga
    10201 ccgtctgcaa caggactttg tacagtggac acaggagtcg gcggaatcag tcgtgccgga
    10261 tgcagacggt aaaggcgcag gcacactgac ggacgctgct gacaccctgc ttgcccgcta
    10321 cgccaaaaac atgaccgcag acgcccgtaa cggcaggctt gacccggtac tgtgccgcga
    10381 ccacgaaatc aacctgatga tcgacattct ctgccgccgc cgcaagaaca acccggtggt
    10441 ggtgggcgaa gcaggcgtgg gtaaaagtgc cttaatcgaa gggctggcgc tgcgcatcgt
    10501 ggcaggccag gtgccggaca aactgaaaaa caccgatatc atgacccttg acctgggcgc
    10561 attgcaggcc ggggcgtcgg tgaagggcga attcgaaaaa cgcttcaagg ggctgatggc
    10621 ggaggtcatt tcctctccgg tgccggtcat tctgtttatt gacgaagcac ataccctgat
    10681 tggcgcgggc aaccagcagg gcgggctgga tatctccaac ctgctcaaac cggcgctggc
    10741 gcgcggcgaa ctgaaaacca tcgccgccac cacctggagc gaatacaaaa aatacttcga
    10801 gaaagatgcc gctctgtcgc gccgcttcca gttagtgaag gtcagcgaac ccaacgccgc
    10861 tgaagccacc attattctgc gtggtctgtc ggcggtctat gaacagtctc acggcgtgct
    10921 gattgatgat gacgccttgc aggccgcagc aacgctgagc gaacgttatc tctccgggcg
    10981 tcagttgccg gacaaagcca ttgatgtgct ggataccgcc tgcgcccgcg tcgccattaa
    11041 cctttcctcg ccgccgaagc aaatctcggc gctgaccact ctgagccacc agcaggaggc
    11101 ggaaattcgc cagcttgagc gcgagcttcg catcgggctg cgtaccgaca catcacggat
    11161 gaccgaggtg ctggtgcagt atgatgaaac gctgacggcg ctggatgaac tggaggccgc
    11221 ctggcatcag cagcaggcgc tggtacagga gattatcgcg ctgcgccagc aattgctggg
    11281 cgtggcagag gacgatgcgg cgccgttgcc ggacgcagat accgtggagg atacgccgcc
    11341 agagtcagag tcagagtcag agtcagagtc agaacaggat aataccggtg ccgtaccggc
    11401 tgatgaggcc gacagagaac agccggaaga gaccgctgaa acagtttccc cggtacagcg
    11461 gctggcccag ctcactgccg aactggacgc cctgcataac gaccggttgc tggtctcccc
    11521 gcacgtcgat aaaaaacaga ttgcggcggt gattgccgaa tggaccggcg tgccgctcaa
    11581 ccgcctgtcg cagaatgaaa tgtcggtcat caccgacctg ccgaaatggc tgggcgacac
    11641 catcaaaggc caggacctgg cgattgccag cctgcataag catctactga ccgcacgcgc
    11701 agacctgcgt cgtccgggac gcccgctcgg tgcgttcctg ctggctggcc ccagcggtgt
    11761 gggtaaaacc gaaaccgtcc tgcaactggc agagctgctc tacggcggtc gccagtacct
    11821 gaccaccatc aatatgtccg aattccagga gaaacacacc gtctcgcggc tgattggttc
    11881 gcctccgggc tacgttggct acggtgaagg cggcgtactg accgaagcga ttcgccagaa
    11941 accctactcg gtagtactgc tcgatgaagt ggaaaaagcg cacccggatg tgctcaacct
    12001 gttctaccag tcgttcgata agggcgaaat ggcagacggt gaaggccgcc tgattgactg
    12061 caaaaatatc gtcttcttcc tgacgtccaa cctcggctac caggtaatag tcgagcacgc
    12121 cgatgacccg gaaaccatgc aggaagaact gtatccggtg ctggccgact tcttcaaacc
    12181 tgccctgctg gcgcgtatgg aagtggtgcc gtatctgccg ctgtcgaaag agacgctcgc
    12241 caccattatc gccggaaaac tggcccgtct ggataacgtg ctgcgcagtc gctttggtgc
    12301 agaagtggtc attgaaccgg aagtgacgga cgaaatcatg agccgcgtca cccgcgcgga
    12361 aaacggcgcg aggatgctgg aatcggtcat cgatggcgac atgctaccgc cgctctcgct
    12421 gctgctgttg cagaaaatgg cggctaacac ggcgattgcc cggattcggt tgtcggcggt
    12481 ggacgacgca tttacggcag acgtggaaga tgctcagaac gacgagtccg tcacaaagga
    12541 tgaaacggtt ttatgagcag gttgcgcgtt ttccggcaga caggatggtt tatcgcgggc
    12601 ctgatgacgg gcttacccgc cactgccgcg cccgcagaaa catcgtcaat ggccggtgtg
    12661 gcggtcgccg tcgccaccac gcccccgccg gatgcgacag ccacgttaca ggccatgcag
    12721 tcctgtcggc gggagtccgc ggcgctggag cggcttgact gctatgaccg cctgctggca
    12781 ccgctgtccc cgtcagggtt tgacggggcg ctggtcaaag ccggttttgt gggcgaggcc
    12841 tggacccgcg ccacagagca ggaaaaacgc cgcgagggca acaccacaga actgctggtg
    12901 acgcaggtgc cgggggagcg cccgacggtg gtgataacca ccccggctat cggccatgtt
    12961 ccgccgcgcc cggtgctgat gttcagttgc gtggacaata tcacccggat gcaggtggcg
    13021 ctgatgcacc ctctggatgt ccatgatatc gccgtcactc tgaatgccga cagccgggca
    13081 ttgcgtagcc actggtttgt gcgcgagaac ggcaccctgc tggagtccag ccgggggctg
    13141 tccgggattg atgaaattaa gcaactgttc ggcgcaaaaa cgctgacagt ggataccggc
    13201 gcagataacg ccgcaggaaa gctgaccttt aacattgacg ggctggcaag ggcgattgcc
    13261 cccctgcgcg ttgcctgcca ctgggcggga gaataaaaat atggcaatga acttacgcga
    13321 tccgaatgtc tggatatcgc acctgctgga aaacctgccg gaagaaaaac tggcatcggc
    13381 gctgaaagat gacaacccgg actgggagta tatcgacggc gaaatcgtca aactgggatc
    13441 tctggcccac agtcaactgg atatcccgga actccagcgc cgggggctgg tcattctggc
    13501 ttcggagagt aaggactttc gtctgctggc gcatctgctg agaaccctgc aacacgccgg
    13561 tgatcctcat ctcgcattgc gcctgctggc gctgtatgtg gagcattact ggtcggtggc
    13621 tgcgccgcag aatatggcac ataaaaaacg ctttgccagc caggtcatta aacgctttga
    13681 aacaggtatt gaaggctttt cacagaacgc cgccacagcg cagcacgacg cgctgtcagg
    13741 ggaactggcg aaactggcgc agtgctggca gtcatataac gccccggaac tggcactggc
    13801 taccgatgat ctttttgccc tgtaccagcg tgcgtttaat cgtgcggcac cggctcctgc
    13861 ccccactccg gcggcctccg atagttcacg acagaccacc gccacgtctg aaagcggcgt
    13921 gacgcaaccc agtgctccgg ctccccaaat cgccatcgac agtcacgacg acaaagcctg
    13981 gcgcgacacg ctgttaaaag tggcggctat tttatgtgaa cgccagccgg actcgccgca
    14041 gggctatcgc ctgcgccgcc atgccctgtg gcaatccatc accagtacac cccaggcgga
    14101 aagcgatgga cgtaccccat tggctacggt ctctgccgat atggtggcgg attaccagtc
    14161 ccggcttgcc agcgcggata tggcgctgtg gcagcaggtt gagaaaagcg tattgctggc
    14221 tccttactgg ctggacggtc actgtctttc tgcacagacg gcactgcgtc tgggttacaa
    14281 acaggtggca gacaccatcc gcgatgaggt catccgcttc cttgagcgtc tgccccagct
    14341 taccgggctg ctgtttaatg accgcacacc gtttctcagt gagcagacga aacaatggct
    14401 ggcagcttcg cccgacggca aagttgcacc ggttgcgcaa atcggtgagg aatcgcaggc
    14461 agccagagcc tgttttgctg ggcagggtct ggaggcggcg ctgcgatatc tggacatgct
    14521 acccgaaggc gatccccgcg atcagtttca ccgccagtac cttgccgcac agttgacgga
    14581 ggaggcgggg ctgatacagc ttgcgcagca acagtaccgg atgttgttga tgatagggag
    14641 tcagatgatg gtgtctgact gggagccatc attacttacg cagcttgaac aaaaattcac
    14701 ggcagaacaa taaaagagaa caggagtttt tgtgttcaga ttaccaacac cccgactact
    14761 cagcggactc aaatcagccc tacgcccggc gatgccccgg tttaaagtct ccgctttctg
    14821 gctgctgata ctggcgtgga tttttctgct ggtatggatc tggtggaaag gcccgacatg
    14881 gacgctgtat gaagaacagt ggctcaaacc actggcgaac cgctggctgg caacggcggc
    14941 atgggggatt atcgccctag tgtggcttac cgtccgggtg atgaagcgcc tgcaacagct
    15001 tgaaaaaatg caaaagcagc agcgcgagga ggccattgat ccgctcagtg tggagctgaa
    15061 cgcccagcag cgttaccttg accgctggct gctgcgcctg caacgccatc tcgacaaccg
    15121 ccgtttcctg tggcagttgc cgtggtatat ggtcatcggc ccggcgggca gtggcaaaac
    15181 gacactgctg cgcgagggtt ttccgtccga cattatttat gccccggagg gcgcacgcgg
    15241 cgcagaacaa cgcctgtacc tcacgcccca tgtcggtaaa caggcggtga tctttgatat
    15301 cgacggcaca ctctgcgctc ccgctgatgc ggatatcctg catcgccgtc tgtgggaaca
    15361 tgccctcggc tggctgaaag aaaagcgcgc gcgccagccg ctgaacggga ttattctgac
    15421 actggattta cccgatctgc ttaccgcaga caaacgccgc cgcgagcatc tgttacagac
    15481 gctgcgcagc cgcttgcagg atatacgcca gcatcttcac tgccagttac cggtttacgt
    15541 ggtactcacc cggcttgatt tattgcaggg ttttgccgcc ctgttccagt cactgaacag
    15601 acaggatcgc gatgcaattc taggggtcac gttcacccgc cgcgcccatg aaaatgatga
    15661 ctggcgaaca gagttgaatg ctttctggca gacatgggtg gatcgaatga acctagcgtt
    15721 gccggatctg atggtcgctc agactcacac ccgcgcgtct ttatttagtt tttcccgcca
    15781 gatgcaggga agccgtgaac cgctggtgtc actgcttgag ggtctgcttg acggcgaaaa
    15841 tatgaacgtg atgctgcgtg gtatctatct cacctcttcg cttcaacgcg gacagatgga
    15901 tgatattttc acccagtctg ccgcccgcca gtaccggctg ggcaataacc cactggcgtc
    15961 ctggcccctg gtggacaccg cgccttattt cacccgtggc ctgttcccgc aggcattact
    16021 cgcagagcct aatctggcaa cagaaagccg cacctggctg atgcgttccc gtcgccgcct
    16081 aacggttttc tctaccacag gaggcgtggc ggcactgctg ctcatcaccg gctggcatca
    16141 ctattacaac ggtaactatc agtccggcat caccgtgctt aagcaggcaa aagcctttat
    16201 ggatgtaccg cctccgctgg gggaagatga ttacggcaac ctgcaactgc cgctgctgaa
    16261 cccggtacgc gatgccacgc tggcctatgg cgactggggc gaccgcagcc gtctggccga
    16321 tatgggactg taccagggac ggcgtatcgg gccttatgtg gaacaaacct atctgcaact
    16381 gctggagcag cgttacctgc cttcgctgtt taacgggctg gtcaaagcga tgaacgccgc
    16441 gccgccggag agtgaggaaa aactcgccgt gctgcgtgtg atgcgaatgc tggaggacaa
    16501 aagcggacgc aataatgagg tggtgaagca gtatatggca aaacgctgga gcgaaaaatt
    16561 ccacggccag cgcgatatcc aggcacaact gatgtcccat cttgactacg cgctggctca
    16621 tactgactgg catgcagagc gtcaggcggg cgacggtgac gccatcagcc gctggacgcc
    16681 atatgacaag ctcgtggtat cagcacagaa agaactgagc aaactgcctg tctaccagcg
    16741 ggtttatcag agcctgaaaa cgcgggcgct gggcgttctt cctgctgacc tcaatctgcg
    16801 tgaccaggta gggccaacct ttgaccaggt gtttacatct gccgatgaca acaaactggt
    16861 tgttccacag tttatcaccc gctatggcct gcaaagctat tttgtaaaac agcgcgatga
    16921 actggttgaa ctgacagcga tggattcctg ggtacttaac ctcacccgca gtgtgaaata
    16981 cagtgacgcc gaccgtgcgg aaatccagca ccagttgacc gagcagtata tcagcgacta
    17041 caccgccacc tggcgggccg ggatggacaa tctgaatatc cgcaattttg agtccatcgg
    17101 acaactgacc ggggcgctgg agcaggttat cagcggcgac cagcctttgc agcgggcgct
    17161 gaccgtgctg cgtgacaaca cacagccagg cgtcttttct gaaaaactct ctgccaaaga
    17221 acgggaggaa gccctggcag agccggatta ccagttactc acccgcctcg ggcatgaatt
    17281 cgccccggaa aacagtaccc tggcagtaca gaaagacaaa gaaagcacga tgcaggccgt
    17341 gtatcagcaa ctcaccgagt tgcaccgcta cctgctggca atccagaacg cgcctgtacc
    17401 agggaaatcg gcgctgaaag ccgtgcagtt acggcttgat cagaacagca gcgatccgat
    17461 attcgccacc cgccagatgg caaaaacgct gcctgctccg ctcaaccgct gggttggcag
    17521 actgactgac caggcctggc atgtggtgat ggtggaggct gttcattata tggaagtgga
    17581 ctggcgcgac agcgtggtga aaccgtttaa cgagcaactg gcaaataact atccgtttaa
    17641 tccgcgttct gcacaggatg cctcactgga tgccttcgaa cgcttcttta aaccggatgg
    17701 catactggat accttctacc agcagaacct gaagctgttt atcgataatg acctgagtct
    17761 ggaggatggc gataacaacg tcattattcg cgaagatatt attgcgcaac tggaaactgc
    17821 gcagaaaatc cgtgacatct tcttcagcaa acagaacggt ctgggaacat cctttgccgt
    17881 ggaaacggta tcgctttccg gcaataaacg ccgcagtgta ctgaaccttg acggtcagtt
    17941 agtcgattac agccagggcc gtaactatac cgcccatctg gtctggccta acaacatgcg
    18001 cgaaggcaac gaaagtaagc tgacgctcat cggcaccagc ggcaacgcgc cgcgcagtat
    18061 cagcttcagc gggccgtggg cgcagttccg cctgttcggg gccggacaac tgaccggagt
    18121 acaggatggc aactttaccg tgcgctttag cgtggacggt ggcgcgatga cctaccgtgt
    18181 gcataccgac accgaggata acccgttcag cggtgggttg ttcagccagt ttggtctgtc
    18241 agacacactg tactgaggag gcagggatga atagtaacgt actgacacaa actatcgtta
    18301 ccggcagtga cccgcgcggg ctgccggaat tcagcgccat tcgcgaggaa ataaacaaag
    18361 ccagccaccc gtcacagcct gagctgaact ggaaactggt ggagtcgctg gcgctggcga
    18421 tttttaaagc taacggtgtg gatttacaca ccgccaccta ctatacgctt gcacggacac
    18481 ggacacaggg actggcgggc ttttgcgaag gtgcggaact gctggcggca atggtaagcc
    18541 acgactggga taagttctgg ccacagggcg gtccggcgcg tactgaaatg ctggactggt
    18601 ttaactcccg aaccgacaat attctgcgtc agcaaatctc ctttgcggaa tccgacctac
    18661 cactgatcta ccgcacagag cgggcattgc agcttatctg cgacaagctc cagcaggtag
    18721 agctgaagcg cgttccgcgc gtggagaatc tactctattt tatgcagaac acgcgtaaac
    18781 ggcttgaacc ccagccgaag agtaacgcag agaacgccgc acagaccacg gtcagaacgc
    18841 tgatttatgc cccggaaaca atggcttcct ccacgccaga agcggtagtg cctcccctgc
    18901 ccgacctgcc tgagatgaaa gtggaagtgc gcagtctgtc agaaaatccc ccacaggcca
    18961 gtgtgatgaa gcaaggcagt acggtaagag ggtttatcgc agggatcgtc tgttcagtgg
    19021 ctatcgcctc agcattgtgg tggtggcagg tctatccggc gcagcagcaa ctgttacagg
    19081 ttaacgacac cgcgcagggc gcagcaacgg tgtggatggc ctcacctgaa cttgaaaact
    19141 atgagcgcag gctgcatcag cttcttggcg tctccccggt acaaccgctg gaaaccggga
    19201 tgcagatgat gcgtgttgcc gacagtcgct ggccggaaag cctgcaacag cagcaggcct
    19261 cgacacaatg gaatgaggca ctcaaaaccc gcgcacagag tagcccacag ttgcgcggct
    19321 ggttgcagac ccgccaggac ttacatgctt ttgctgatct cgtgatgcag cgcgagaaag
    19381 agggattaac cctttcctat atcaaaaatg tcatctggca ggcggagcgg ggactggggc
    19441 aggaaacacc tctggaatcc ctgctgacgc agtaccagga tgcccgtgcg cagaagcaga
    19501 atacagatgc gctggaaaaa cagattaacg agcggatgga cggcgtgtta agtcgctggc
    19561 tgctgctgaa gaataatacg ataccgacga taaaaaaaga cat
//
LOCUS       DAZUCC010000082        19558 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZUCC010000082 DAZUCC010000000
VERSION     DAZUCC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.082"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..206
                     /locus_tag="ACHY09_002507"
     CDS             102..206
                     /locus_tag="ACHY09_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885465.1"
                     /translation="MENNSRTMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            464..2662
                     /gene="speF"
                     /locus_tag="ACHY09_002508"
     CDS             464..2662
                     /gene="speF"
                     /locus_tag="ACHY09_002508"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFS3885466.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVT
                     RGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEFAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2659..3978
                     /gene="potE"
                     /locus_tag="ACHY09_002509"
     CDS             2659..3978
                     /gene="potE"
                     /locus_tag="ACHY09_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFS3885467.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKIFSRVSKVDAPVQGMLTIVIIQS
                     GLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFDLKNKHG"
     gene            complement(4035..5675)
                     /gene="pgm"
                     /locus_tag="ACHY09_002510"
     CDS             complement(4035..5675)
                     /gene="pgm"
                     /locus_tag="ACHY09_002510"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFS3885468.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5701..6246)
                     /gene="seqA"
                     /locus_tag="ACHY09_002511"
     CDS             complement(5701..6246)
                     /gene="seqA"
                     /locus_tag="ACHY09_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFS3885469.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6431..7195
                     /gene="ybfF"
                     /locus_tag="ACHY09_002512"
     CDS             6431..7195
                     /gene="ybfF"
                     /locus_tag="ACHY09_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS3885470.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7335..7628
                     /gene="ybfE"
                     /locus_tag="ACHY09_002513"
     CDS             7335..7628
                     /gene="ybfE"
                     /locus_tag="ACHY09_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFS3885471.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7768..8298
                     /gene="fldA"
                     /locus_tag="ACHY09_002514"
     CDS             7768..8298
                     /gene="fldA"
                     /locus_tag="ACHY09_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFS3885472.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8508..8594
                     /gene="uof"
                     /locus_tag="ACHY09_002515"
     CDS             8508..8594
                     /gene="uof"
                     /locus_tag="ACHY09_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFS3885473.1"
                     /translation="MIRIISRANSVTSPNEVNRLVTGQIPHD"
     gene            8587..9033
                     /gene="fur"
                     /locus_tag="ACHY09_002516"
     CDS             8587..9033
                     /gene="fur"
                     /locus_tag="ACHY09_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFS3885474.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9117..9443)
                     /gene="chiQ"
                     /locus_tag="ACHY09_002517"
     CDS             complement(9117..9443)
                     /gene="chiQ"
                     /locus_tag="ACHY09_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFS3885475.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9493..10899)
                     /gene="chiP"
                     /locus_tag="ACHY09_002518"
     CDS             complement(9493..10899)
                     /gene="chiP"
                     /locus_tag="ACHY09_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFS3885476.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDIYDGTAWLQALTFGYRAADVVDLRLEGSWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11348..13012)
                     /gene="glnS"
                     /locus_tag="ACHY09_002519"
     CDS             complement(11348..13012)
                     /gene="glnS"
                     /locus_tag="ACHY09_002519"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFS3885477.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGSFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSCEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13215..15161)
                     /gene="nagE"
                     /locus_tag="ACHY09_002520"
     CDS             complement(13215..15161)
                     /gene="nagE"
                     /locus_tag="ACHY09_002520"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFS3885478.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDVMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15494..16294
                     /gene="nagB"
                     /locus_tag="ACHY09_002521"
     CDS             15494..16294
                     /gene="nagB"
                     /locus_tag="ACHY09_002521"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS3885479.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16354..17502
                     /gene="nagA"
                     /locus_tag="ACHY09_002522"
     CDS             16354..17502
                     /gene="nagA"
                     /locus_tag="ACHY09_002522"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS3885480.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            17511..18731
                     /gene="nagC"
                     /locus_tag="ACHY09_002523"
                     /gene_synonym="nagR"
     CDS             17511..18731
                     /gene="nagC"
                     /locus_tag="ACHY09_002523"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFS3885481.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18779..19531
                     /gene="nagD"
                     /locus_tag="ACHY09_002524"
     CDS             18779..19531
                     /gene="nagD"
                     /locus_tag="ACHY09_002524"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFS3885482.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         4782 a         4972 c         4900 g         4904 t
ORIGIN      
        1 aaattaagat tgatcatttt ttattgatca ccttcacagt tcaaccgtat ttcctggata
       61 gaataccttc accttcattc acatcaggaa aggtaaatta aatggaaaat aacagccgca
      121 ctatgcccca tataaggcgg acaactcata ttatgaagtt tgctcatcgc aatagcttcg
      181 actttcactt cttcaatgcc cgttagtcta ccgactaagg gcacttcagc gtacaggtct
      241 tcctgactct ctgtattaca ggcatatgcc tgtattccgc agtgctcatt ctctgttaac
      301 ccttgtatta tcagccaccg gactgataag ggtttctgca ttctgaaaaa agcaaacttc
      361 tgattaattt atcagcgggg taactttgct ttttttccgg cacgatcgat ttctcattcg
      421 agaaattgag gacctgctat tacccaaaat aaagagatgg aaaatgtcaa aattaaaaat
      481 tgcggttagt gattcttgcc cggactgttt taccacgcag cgagaatgta tctatattaa
      541 tgaaaacagt aatatcgatg tggcggcaat agttttatcg ctcaacgatg ttacacgcgg
      601 aaaactcgat gaaatcgatg ccacgggtta tggcatcccg gtatttattg ctactgaaaa
      661 tcaagaacgt gtacccgcag agtatttgcc ccgtatttcg ggtgtctttg agaattgcga
      721 atcgcgacga gaattttatg gtcgtcagtt agaaaccgca gccagccatt atgaaacgca
      781 actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggta acagcgcgtt
      841 tgattgcccc ggtcatcagg gcggcgaatt tttccgtcgc catcccgcgg ggaatcagtt
      901 tgtggaatac tttggtgagg cgctgttccg tgctgacttg tgcaacgccg acgtagcgat
      961 gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg cggcaaaagt
     1021 gtttaatgcc gataaaacat acttcgtttt aaatggcact tcatcttcta acaaagtggt
     1081 tttaaacgcc ctgctgacac cgggtgatct ggtgctgttt gaccgcaata accacaaatc
     1141 taaccaccac ggagcgttgc tacaggctgg cgcgacaccg gtttatctgg aaaccgcacg
     1201 taacccgtat ggttttatcg gtggtattga cgctcactgt tttgaagaaa gttacctgcg
     1261 tgaactgatc gcggaattcg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc
     1321 cgttattcag ctaggcactt acgacggtac gatttataac gcccgtcagg tggtggataa
     1381 aattggtcat ctgtgtgact acatcctctt tgactccgca tgggtcggct atgagcagtt
     1441 tatcccgatg atggcggact gttcaccact gttgctggat cttaatgaga acgatccggg
     1501 tattctggtt acgcaatctg tgcataaaca gcaggctggt ttttcccaga cttcacaaat
     1561 ccataaaaaa gacagccata tcaaagggca acagcgttat gtaccgcaca aacgcatgaa
     1621 caacgccttt atgatgcatg cctccaccag cccgttctat ccgctgtttg ccgcactgga
     1681 tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt
     1741 aaatggcatt aatgcccgca aactaatcct cgataactgt cagcatattc gtccattcgt
     1801 acctgaactg gtggatggta aaccctggca gtcgtatgaa acggcgcaaa ttgcggttga
     1861 tctgcgcttc ttccagtttg taccaggtga gcactggcat tcttttgaag gctatgcaga
     1921 gaatcaatac tttgtcgatc cgtgcaaact gttgctgaca accccaggta ttgatgcacg
     1981 taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga
     2041 aaatggcgta gtgccggaaa aatgcgatct taactccatc ctctttctgc tgactccggc
     2101 agaagatatg gccaaacttc agcaacttgt tgctctgctg gtacgcttcg aaaaactgct
     2161 tgagtccgac gcgccattat cagaagtact gccttccatc tacaaacagc atgaagagcg
     2221 ctacaccggt tataccctgc gtcagttgtg tcaggaaatg catgatttgt atgcccgtca
     2281 caacgtgaaa caactgcaaa aagagatgtt ccgtaaggaa cacttcccac gcgtaagcat
     2341 gaatccacaa gaagccaact acgcctattt acgcggtgaa gtggagctgg ttcgtctgcc
     2401 ggatgcagaa ggccgtatcg ctgccgaagg tgcgcttcct tatcctccgg gtgtgctgtg
     2461 tgttgttccg ggtgaaatct ggggtggtgc tgttctgcgt tacttcagcg ctctggaaga
     2521 agggatcaac ctgctgccag gttttgcacc agagctgcag ggtgtctata tcgaagaaca
     2581 tgatggtcgt aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc aaagcaccct
     2641 gttgaaaggg gaaaaattat gagtcaggct aaatcgaata agatgggcgt cgttcagtta
     2701 accatactga cgatggtcaa catgatgggc tccggtatca tcatgctgcc gacaaagctt
     2761 gccgaagttg ggacaatctc aattatctcc tggctggtga cagccgtagg ctcaatggca
     2821 ctggcatggg cattcgcaaa atgcggtatg ttcagccgta aatcaggcgg tatgggcggt
     2881 tatgcggagt atgctttcgg taaatccggg aactttatgg cgaactacac ctacggtgtc
     2941 tcgctgctga ttgctaacgt cgcgattgct atttcggcgg ttggttacgg caccgaactg
     3001 ctcggcgcga gtttgtcgcc agtgcagatt ggtcttgcga ccatcggagt gctgtggatt
     3061 tgtaccgtgg ctaactttgg tggtgcgcgc attaccgggc aaatcagtag cattactgtg
     3121 tggggggtca ttattccggt cgtcggtctg tgcattatcg gctggttctg gtttagcccg
     3181 acgctatacg ctaactcatg gaacccgcac catgtgccgt tctttagcgc tgtaggttct
     3241 tccatcgcca tgacgttgtg ggctttcctc ggtctggagt cagcctgtgc caacgccgaa
     3301 gtagttgaaa acccggaacg taacgtgccg attgcagttc ttggcggtac gttaggtgcg
     3361 gcggtgattt atatcgtctc caccaacgtg attgccggga ttgtgccaaa tatggagctg
     3421 gcaaattcaa cggcaccatt tggtctggcc ttcgcgcaga tgttcacgcc ggaagtgggt
     3481 aaagtcatta tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg ctggcagttc
     3541 accattgccc aggtgtttaa atcttctgcc gatgaagggt acttcccgaa aattttctcc
     3601 cgtgtgagca aagtcgatgc accagtacag ggaatgctga ccattgtcat catccagagc
     3661 ggattatcac tgatgaccat tagcccgtcg ctgaacagtc agttcaacgt gctggttaac
     3721 ctggccgtgg tgaccaatat cattccgtat attctgtcaa tggcggcatt agtcattatt
     3781 cagaaggtcg ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt tgcttttgtt
     3841 ggcgcgatgt acagctttta tgcgctgtac tcatccgggg aagaagccat gctatacggt
     3901 tccatcgtga ccttcctcgg ttggacactg tatggtctgg tctcaccacg ctttgacctg
     3961 aaaaataaac acggttaatt aagcaaagca aaaaagggcg atcttccgac cgcccttttt
     4021 cttatttgat atgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc
     4081 tcaatctgct tgcgatgttc ttcaccaagg aagctttcgc agtagatctt atatgcgtct
     4141 tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgccg
     4201 atagaagcgc cattacccgg tgccgccgtc agacgcgcgg tgatcgggtc acctgccagg
     4261 gtgctggcgc tcaccatttc cggagacagc ttagacaacg ccgctttttg tgcggaagtc
     4321 gctgctgcct gcaaacggtt atagctcggc gcaccaaagc gttttgccag ttcgttatag
     4381 tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacataatg
     4441 ataccgtctt tatcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcgcctgca
     4501 ctctcttcgc cgccaaagcc gaagctgcca tcgaacaaac catcaacaaa ccatttgaaa
     4561 cctaccggta cttctaccag cttacggccc aaatcattga ccacacggtc gatcatcgca
     4621 gatgaaacca gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac
     4681 aggtagttga tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg
     4741 ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcagt
     4801 gccagcaggc ccgccatcgc acactcggag gagcagtcca tacggatcgc accgtcttta
     4861 tcgaggtgca taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg
     4921 ttgtaatact cgccaatgcg cttccagtat tcgataccgg aaccacccag cggatcaacg
     4981 cccagcgtca ggcccgcttt ctgaatcgcc gccatatcaa cgatatcggc cagcccttcc
     5041 acaaacggct gcaccagatc ctgctctttc acatgaccgg atgccatcgc ttcgtcgagg
     5101 gagatacgct tcacgccttt caggccatca gccagcagtg cgttggctct gtcttccacc
     5161 actttagtga cgttggtatc agccgggcca ccatttggcg gattgtactt gataccacca
     5221 tcttccggcg ggttatggga cggtgtaatc acgataccgt ctgccagtgg gccacctttt
     5281 ttattgtgaa ccagaatggc attggaaatg gcaggtgtcg gagtgaagcc attgttttcc
     5341 tgcacaatga catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc
     5401 tcggacaggg cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca
     5461 cgttcttcag caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc
     5521 gctgcactgc cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct
     5581 tctggtttca gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt
     5641 tgtgcaggtt ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt
     5701 ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat
     5761 gtgttcgatc atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt
     5821 accaggcaca tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc
     5881 cgcaaagtaa acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc
     5941 gtcaagagaa tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc
     6001 tgcgtattcg tccgaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt
     6061 gaccggcttc gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc
     6121 agcaggctgt gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc
     6181 gccgatatgc ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt
     6241 tttcatctta atccagtgca gtgtcttagc tgaatacaag tcgccaggag tatatgaata
     6301 aatcccggca gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt
     6361 tgttgcagcc ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa
     6421 gaactttgcc atgaaattga atatccgcgc gcaaactgca caaaaccagc acaataattc
     6481 tcccatcgtt cttgtccatg gtctgtttgg cagcctcgac aaccttggcg tactggctcg
     6541 cgatctggta aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc
     6601 gagagatccg gtaatgaatt acccggcgat ggcgcaggat cttgttgata ccctggatgc
     6661 acagcagatc gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc
     6721 tcttactgca ctagcccccg atcgcattga caagctggtg gcgattgata ttgcgccggt
     6781 cgattatcac gttcgccgtc atgatgagat tttcgcggcc atcaacgcag tcagtgaatc
     6841 agacgcacaa acacgccagc aagcagcggc aataatgcgc cagcatctta atgaagaagg
     6901 ggtgattcag tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccagt
     6961 attgtgggat cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcatcc
     7021 taccctgttt atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt
     7081 actggcacaa tttccacagg ctcgggcgca tgtgattgca ggcgcgggtc actgggtcca
     7141 tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa
     7201 ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg gacgttggcg tgactcatcg
     7261 cgctgatgta ttatggcgcg ctatccatcc gggcaggcgc ctggctgatt gtttcccccg
     7321 aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc
     7381 acgagcgtcg accgggacga ccgaaaacta atccgctttc gcgcgatgag cagctgcgta
     7441 ttaataaacg caaccaacta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga
     7501 agctgaacgc ggaagctgtc gaggcactga acgagctggc ggaatcgcgc aatatgagcc
     7561 gtagcgagct gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca
     7621 tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg
     7681 caagactgcc tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc
     7741 aataagtttc aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac
     7801 accggtaata ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc
     7861 gatgtccatg acattgcaaa aagcagcaaa gaagatctgg aagcatatga cattctgctg
     7921 ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg actgggatga cttcttcccg
     7981 actctcgaag agattgattt caatggtaaa ctggttgcgc tgtttggttg tggcgaccag
     8041 gaagattacg cagaatactt ctgcgatgca ttaggcacca tccgcgacat cattgaaccg
     8101 cgcggcgcaa ctatcgttgg tcactggcca accgcgggct accacttcga agcgtcaaaa
     8161 ggtctggctg atgatgacca ctttgtcggt ctggctatcg acgaagatcg tcagccggaa
     8221 ctgactgctg aacgtgtaga aaaatgggtt aaacaaattt ctgaagagct gcatctcgac
     8281 gaaattctca atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat
     8341 aatgccaatc aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt
     8401 cccggtgttc aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa
     8461 tatttgccag ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta
     8521 tctcaagagc aaattctgtc acttctccta atgaagtgaa ccgcttagta acaggacaga
     8581 ttccgcatga ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct
     8641 cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc atcacgtcag tgcggaagat
     8701 ttatacaaac gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta
     8761 ctgaaccagt ttgacgatgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc
     8821 gtatttgaac tgacacagca acatcaccac gatcacctga tctgcctcga ctgcggcaag
     8881 gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc gtgaaatcgc cgcaaaacat
     8941 ggcattcgcc tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc
     9001 cgcgaagatg agcacgcgca cgaaggcaaa taagccagcc tgaacgagaa aagccaacct
     9061 gcaggttggc ttttttatac aagggaaata agaaaagcga gagttacagc tctcacttat
     9121 ttgttattac tgcgaatttc ctgccagacc ttatcacaat cggctttcac cgcctgatca
     9181 tttccggttt gcgttgcctg aatacactgc tgataatcca gtacgcggac actttcctgc
     9241 tcggcaaact gctgatgttg tttctctttc ttcagcacgt ttaacacgct ctggcaggct
     9301 tcaatttttt ccggcgaacc ttgcgcggtg ttgatacagg cgctgtaagc ctctttcaga
     9361 cgactgtctt cctgcggtgc ggttgattgc gcacaagcta ccagccccga tgccatcatg
     9421 gcgatgagaa tcagtttttt catagcaaat tcctcaaatg tgcggcaggc acaaaacctg
     9481 ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc ataaatttca cgtcgcgctc
     9541 atcctggaag atgttaccgt aaccgccgcc ccagcttggg atgtcggagt ggttgtcgta
     9601 ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa tggtgtacac
     9661 cgcatccagg ctgtaggcag actcttcaat agtccggttt ttgtcgtagt acgcatccgg
     9721 attgctctgc caggtcgcag gtttagcatc ccatgcgtaa acgtaggaag cgccgatggc
     9781 gaagcctgga agattccagt ttttcaggtc atacatcgca ccgaagaaga ccgctttttc
     9841 gccgttggcg ttgaagtcag aacggttatc ccaccagata tccaggcgac cgttcgagga
     9901 agcgtaggtt ggagtcatac gttgcaggaa gtatccctgc tgaccgtcgg ctttaaccca
     9961 gctgccttcc agacgcaaat ccactacatc agccgcccgg taaccaaagg tcaacgcttg
    10021 cagccaggca gtgccgtcgt aaatgtcgtt gacgctgcga tcgtcaactt tatcgcgcgt
    10081 accgtagaac tggtagctgg tggttaacgg gctaccggcg atatcaaatt tgtagctggc
    10141 tttggcaaaa tattgatcta tatacccttc cgcctgacca aatgccgctt ccagtacaaa
    10201 gttatttttg aagtcgtact tcgcaccgat ggagtgcaga taatcaactt tggtagtttt
    10261 atcgttctga taaaactcat ccatttcgag atgccacggt gctttgtatt cgttggtcca
    10321 catgtaggag aaactcaacg caccagcatc gccgtaatca aaattcgccc cggcttccgc
    10381 accctgataa gtacccggca taaagctcca gtgcggcgct aacagcgtct gaccagttgg
    10441 ctgaatgtaa cctgccctcg cccacaccgg accgtattta aatttggccg ctgctttata
    10501 caggcttata ccgcttttgt cgccggacca gtcttcgtca taggctttat tgctttttga
    10561 aaacgcgatt tcgttcgggt gagagctgtc accgttttcc gccatttcaa tcgccgtaaa
    10621 cgcagcaata tcaaggccga acatatcagc agcataaccg gactgaaaat cgaggttggc
    10681 attccaggtg gagtgagaaa ggttggtttt gtatttgtcg ccgtcggtaa catctttacg
    10741 gtcgcgttca cgctgccagt aatagatacc gccggttaag gttgaatcgt cgatgaatcc
    10801 ttcggcgcga gcctccggca ttgccataaa gcccgacatt gctgtaacac cggcgatagc
    10861 cagcgccagc gtactacgtt tgccactaaa cgtacgcatg ggttaatcct ctttgacgta
    10921 taaattgctg caccaaaggt gaacagcgaa aaaaataaaa attaaaaaag tagcgttgag
    10981 aaaagtctcg ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt
    11041 ctgtaaccag aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag
    11101 gcttaagttt gcagttgaaa ggcttgcaga agaataaaag cgtgaatttc cagtaagatg
    11161 aaaacggtta cattttattt aattatggtt tgtgatttta aatttgtttt atggataaat
    11221 tgcgaagcgg aattaattcg cgaagaaaag cagaaaaaaa cgccgcagag cgcggcgttt
    11281 gggattgttt gcatatttct tgccggatgt ggcgtaaatg cctcacccgg cctggtagaa
    11341 cttaaaacta ctcgcctact ttcgcccagg tatcacgcag ccctacggtg cggttaaata
    11401 ccggtttttc ggcagtggaa tggcggctgt cgaggcagaa ataaccttca cgctcaaact
    11461 ggaatgcttt acccgctacc gcgtctttca gcgacggttc agcgaaaccc tgtttgatca
    11521 ccagcgattc cgggttaatc accgacagga aatcatccgc agcacctggg ttaggcacgc
    11581 tgaacagacg gtcatacaaa cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
    11641 gaataacacc tttcacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
    11701 aagtacagaa gatggtggtg atattacctt cggcatcttt ctcgacgcgt tctgccttaa
    11761 tcacgtaagc attacgcaga cgcacttctt tacccaacac cagacgtttg tactgcttgt
    11821 tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc acagctaaac ggcacctgac
    11881 ggctacccat ttccggtttg ttcggatggt tcggcatggt gaccatttcg ccttcgccct
    11941 ggtagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggggcatttt
    12001 cgttgagatc ttcacggatg caggattcca gtgacgccat ctcaatggtg ttgtcctgct
    12061 tggtcacacc aatgcgtttg cagaactcac gaatagaagc cgcagtgtaa ccacgacgac
    12121 gcagaccaga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttatcggtca
    12181 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
    12241 cgtactggcg cgggtgaaca ggaatggtga tgttgtccag tacccagtcg tacagacggc
    12301 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagc gcatcgctga
    12361 tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
    12421 cagcaaattt aatgcggtac agcaccggat cgcgcattac gataaatggc gacgccatgt
    12481 cgattttcgc acgcaggcag gctttacctt cttcaaaact accggtacgc attttttcga
    12541 acagcgccag gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggctgcg
    12601 tcagggtgcc gcggtattcg cggatctgtt ccggtgtcag ttcatcaacg tacgccaggc
    12661 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
    12721 ggacgttacc agaccagtga aaacctaacc actcaacgtc gtttttgatc gactcaacat
    12781 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
    12841 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc
    12901 cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg gccagatctt
    12961 catcgatgat ctgacgaata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
    13021 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac
    13081 tgttagttag tgaaaatcga aaatctcact gagattatgg ggatgattga tgaaaaaaaa
    13141 agcggcagag aagatctcca ccgcttgtca ttgttggatg cgacgctcaa gcgtcgcatc
    13201 aggcataaag cagattactt tttgatttca tacagcggtg tttgacccgc cacaacatgg
    13261 ccctgagctt taatgatcag gccgctgaaa tcgtcgatat tgctgcaaac caccgggcta
    13321 atcatcgagc gggcgttagc gttcaggtaa tccagatcca tttccagaat cggttgtcct
    13381 gcgcttacct gcgcgccctc ttccaccaga cgtttaaagc ctttaccttc cagcgctacg
    13441 gtgtcgatac ccatatggac gacgatctcc gcgccttttt cggtttccag acagaacgcg
    13501 tggttggtgt tgaagatttt cacgattgtc cctgcggctg gtgatacgac gattttatct
    13561 gtcggtttca ccgccacacc gtcacccacc gctttgctgg cgaatgcttc gtcaggaacc
    13621 tgatccagtg ccacaacatc accggtaatc ggcgatacca gctccgcgat agacaccgcg
    13681 tttggtacag cctgcggttt tgctacaggc gcggcagttg caggcgcagt ttcagctgac
    13741 gcagcggcta ccggaccacg ggcaacgact ttcttcatcg catcgccgat ggattctgct
    13801 ttcgcgccca caatcacctg aatagtctgt ttgttcagtt ttactacccc agaagccccc
    13861 agacgtttac acatcacatc gttgacgcga gctgagtcaa ccacggtaag gcgcagacga
    13921 gtaatacagg cgtcaatggc tttcaggttg tcagtaccgc caaccgcagc aatatagttg
    13981 gttgccagtt gattgagacc ttcttccgtg ttgctgttgg cttcttcagt aacgatctcg
    14041 tcttctttat cttcacggcc cggcgttttc aggttgaaca tgcggataac caaactgaac
    14101 accacgaagt agatagcgaa gaagacaaca cccatcacca gcagcatcca gacgttctgg
    14161 ctggcggccg gcaggttata catcaacgcg tagtcgatag cccccgcaga gaaggagaag
    14221 cccgcgtgga tacccagcag cgttgccaca aacaggctga taccggtcag cagtgcgtgc
    14281 aggaggtaca gcagcggcgc aaggaacatg aacaggaatt ccagcggctc agtcacaccg
    14341 gtcaggaacg cagtaacagc aacagaaagc agcataccgc caaccatcgg acgacgctct
    14401 ttcggtgctg caaagtacat cgccagcgcc gcacccggca gaccgaacat catgatcggg
    14461 aagaagccgg acatgaacat ccccgcggtg ccgtcaccgg catagaagcg gttaatgtcg
    14521 ccgtggaaaa ccgtacccgc cgcgttggtg aattcaccaa tctggaacca ggcgatggtg
    14581 ttcagtacct gatgcagacc ggttgggatc agcagacggt tgatgaaacc aaagataccg
    14641 gaacccagcg cgcccgcaga aacgatccac tcgccgcctg catggatagc gtgctgtacc
    14701 ggcggccaga cgtaaccaaa aatggccgcc agcaccaggc agaagaagcc ggtggcaatc
    14761 ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg gcagtttaat atcggaccaa
    14821 cggttatagg ctgcaccacc gaccagaccg gtaatgatac ccgccagtac acccatgtta
    14881 atttctgggt tgatggtcac catcgctttg gttaacacaa agtaacctac cgcacccgcc
    14941 agtgccgccg cacctgcgct gtctttcgac cagctggatg ccacgccgat ggcgaagatt
    15001 aatgcgaggt tatcaaaaat cgcaccgccc gcctgggcaa taaacgcaac gttaagtaaa
    15061 tctggctgac cgaatcgcag caacagcgcc gccaccggca gcaccgcgat agggagctgt
    15121 aacgccctac ctagtcgctg gaaaaaacct aaaatattca tcttattccc cctacgagaa
    15181 ccctatttgg ctcgtttaaa gccgtatttt tattttgctg caaattgtac tgccgatgtt
    15241 ctgtaatcag attgttagat catctgctac agagtgtgtg aaaatttaat tcgtatcgca
    15301 aattaaacgc gtgtcttttg tgagttttgt caccaaatat cgttatcatc actccctttt
    15361 actggctaaa ccagaaaact tattttatca ttcaaaaaat caggtcggat tgacgcctgt
    15421 ctgcgcaaat ccaggttacg cttaaagatg cctaatccgc caacggctta cattttactt
    15481 attgaggtga ataatgagac tgatccccct gactaccgct gaacaggtcg gcaaatgggc
    15541 tgctcgccat atcgtcaatc gtatcaatgc gttcaaaccg actgccgatc gtccgtttgt
    15601 actgggcctg ccgactggcg gcacgccgat gaccacctat aaagcgttag tcgaaatgca
    15661 taaagcaggc caggtcagct ttaagcacgt tgtcaccttc aacatggacg aatatgtcgg
    15721 tctgccgaaa gagcatccgg aaagctacta cagctttatg caccgtaatt tcttcgatca
    15781 cgttgatatt ccagcagaaa atatcaacct tctcaacggc aacgccccgg atatcgacgc
    15841 cgaatgccgc cagtatgaag aaaaaatccg ttcttacgga aaaattcatc tgtttatggg
    15901 cggtgtaggt aacgacggtc atattgcatt taacgaaccg gcgtcttctc tggcttctcg
    15961 tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt tctttgataa
    16021 cgatgttaat caggtgccaa aatatgccct gactgtcggt gttggtacat tgctggatgc
    16081 cgaagaagtg atgattctgg tgctgggtag ccagaaagca ctggcactgc aggccgccgt
    16141 tgaaggttgc gtgaaccaca tgtggaccat cagctgtctg caactgcatc caaaagcgat
    16201 catggtgtgc gatgaacctt ccaccatgga gctgaaagtt aagactttaa gatatttcaa
    16261 tgaattagaa gcagaaaata tcaaaggtct gtaattatta tccctgccct ggctccttgc
    16321 tcagggcaat attttttaaa atcgggggtc agaatgtatg cattaaccca gggccggatc
    16381 tttaccggcc acgaatttct tgatgaccac gcggttgtta tcgctgatgg cctgattaaa
    16441 agcgtctgtc cggtagcgga actgccgcca gagatcgaac aacgttcact gaacggggcc
    16501 attctctccc ccggttttat cgatgtgcag ttaaacggct gcggcggcgt gcagtttaac
    16561 gatactgctg aagcagtcag cgtagaaacg ctggaaatca tgcagaaagc caatgagaaa
    16621 tcaggctgta ctaactattt gccgacgctt atcaccacca gcgacgagct gatgaaacag
    16681 ggcgtgcgcg ttatgcgcga gtacctggca aaacatccga atcaggcgtt aggtctgcat
    16741 ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa ttttgtgcgt
    16801 aagcctgatg ccgcgctggt cgatttcctg tgtgagaacg ccgacgtgat taccaaagtg
    16861 actctggcac cggaaatggt tccagcggaa gtcatcagca aactggcaaa tgccgggatt
    16921 gtggtttctg ctggtcactc caacgcgacg ttgaaagaag cgaaagccgg tttccgcgcg
    16981 gggattacct ttgccaccca tctgtacaac gcgatgccgt atattaccgg tcgtgaaccg
    17041 ggcctggcgg gcgcgatcct cgacgaagct gacatttatt gcggtattat tgctgatggc
    17101 ctgcatgttg attacgccaa cattcgtaac gctaagcgtc tgaaaggcga caaattgtgt
    17161 ctggttaccg atgccaccgc gccagcaggt gccaacattg aacagttcat ttttgcgggt
    17221 aaaacaatat actaccgtaa tggactttgt gtggatgaga acggtacgtt aagcggttca
    17281 tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac attgtggtat cgcactggat
    17341 gaagtgctgc gtatggcgac gctctatccg gcacgtgcga ttggcgttga gaaacgtctc
    17401 ggcacgctcg ccgcaggtaa agtagccaac ctgactgcat tcacacctga ttttaaaatc
    17461 accaggacca tcgttaacgg taacgaggtc gtaactcaat aagagaaagt atgacaccag
    17521 gcggacaagc tcagataggt aatgttgatc tcgtaaaaca gcttaacagc gcggcggttt
    17581 atcgcctgat tgaccagtac gggccaatct cgcggattca gattgccgag caaagtcagc
    17641 ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg ctgatcaaag
    17701 aagttgatca gcaggcctcc accgggggcc gccgcgctat ctccatcgtc accgaaaccc
    17761 gcaatttcca cgcaatcggc gtacggcttg gtcgtcacga cgccaccatc accttgtttg
    17821 atctcagcag caaagtgctg gcagaagaac attacccgct gccggaacgt acccagcaga
    17881 cgctggaaca tgccctgctg aatgccattg ctcagtttat tgatagctac cagcgcaagc
    17941 tacgcgagct gatcgcgatt tcggtgatcc tgccagggct tgtagacccg gacagcggca
    18001 aaattcatta tatgccgcat attcaggtag aaaactgggg gctggttgaa gcactggaag
    18061 agcgttttaa agtgacctgt tttgtcggcc acgatatccg cagtctggcg ctggcagagc
    18121 actacttcgg tgcaagtcag gattgcgaag actccattct ggtgcgtgtc catcgcggaa
    18181 ccggggccgg gattatctct aacgggcgaa tttttatcgg gcgcaacggc aacgtcggtg
    18241 aaattggcca tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc aactttggct
    18301 gcctggaaac tatcgccgcc aatgctgcca ttgaacaacg ggtgttgaat ctgttaaagc
    18361 agggctacca gagccgtgtg ccactggacg actgcaccat caaaactatc tgcaaagccg
    18421 cgaacaaagg cgatagcctg gcctcggaag tgattgaata tgtcggtcgt catctgggta
    18481 aaaccatcgc cattgctatc aacttattta atccgcaaaa aattgttatt gctggagaaa
    18541 tcaccgaagc cgataaagtg ctgctccctg ctattgaaag ctgcattaat acccaggcgc
    18601 tgaaggcgtt tcgtactaat ctaccggtgg tacgttctga gttggatcac cgctcagcaa
    18661 tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg tattttgctc cagcatttgc
    18721 tggaaaatta atgtgctttt atagtggcgc ttattgttgt caatattctg ggtagtccat
    18781 gaccattaaa aatgtaattt gcgatatcga cggcgtgctg atgcacgata acgtcgccgt
    18841 accgggtgca gcggaatttt tgcacgggat tatggataaa ggcctgccgc tggtgttgct
    18901 gaccaactat ccttcgcaga ccgggcaaga tctggcgaat cgctttgcca ctgccggtgt
    18961 cgatgtaccg gatagcgtgt tttatacctc cgcgatggcg accgccgatt tcctgcgtcg
    19021 ccaggaaggc aagaaagcgt atgtggtggg cgaaggcgca ctgattcatg aactgtacaa
    19081 agccggtttc actattaccg atgtgaaccc tgattttgtg attgttggcg aaacgcgttc
    19141 ctacaactgg gacatgatgc ataaagcagc ctatttcgtc gctaacggcg cacgctttat
    19201 cgccaccaac ccggacaccc acgggcgcgg tttttatccc gcctgtggcg cgttgtgtgc
    19261 agggatcgag aaaatctccg ggcgcaaacc gttctatgtt ggaaagccga gtccgtggat
    19321 catccgcgcg gcattaaaca aaatgcaggc gcattcggaa gaaacggtga ttgtcggcga
    19381 taacttgcgt accgatattc tggcgggctt ccaggccggt ctggagacga ttctggtgct
    19441 ttctggtgtt tcgtcactcg acgatatcga cagtatgccg ttccgcccaa gttggattta
    19501 cccatcggtc gctgaaatcg acgttatctg aatagcaacc acgcctcagg gcgtggtt
//
LOCUS       DAZUCC010000083        19543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZUCC010000083 DAZUCC010000000
VERSION     DAZUCC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.083"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(168..2600)
                     /gene="metL"
                     /locus_tag="ACHY09_002525"
     CDS             complement(168..2600)
                     /gene="metL"
                     /locus_tag="ACHY09_002525"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFS3885483.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(2603..3763)
                     /gene="metB"
                     /locus_tag="ACHY09_002526"
     CDS             complement(2603..3763)
                     /gene="metB"
                     /locus_tag="ACHY09_002526"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS3885484.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRVALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4040..4357
                     /gene="metJ"
                     /locus_tag="ACHY09_002527"
     CDS             4040..4357
                     /gene="metJ"
                     /locus_tag="ACHY09_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFS3885485.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            4417..5025
                     /locus_tag="ACHY09_002528"
     CDS             4417..5025
                     /locus_tag="ACHY09_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFS3885486.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGELSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGKQQKLKEFDLSNPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5086..5298)
                     /gene="rpmE"
                     /locus_tag="ACHY09_002529"
     CDS             complement(5086..5298)
                     /gene="rpmE"
                     /locus_tag="ACHY09_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFS3885487.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            5501..7699
                     /gene="priA"
                     /locus_tag="ACHY09_002530"
     CDS             5501..7699
                     /gene="priA"
                     /locus_tag="ACHY09_002530"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS3885488.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            7855..8880
                     /gene="cytR"
                     /locus_tag="ACHY09_002531"
     CDS             7855..8880
                     /gene="cytR"
                     /locus_tag="ACHY09_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFS3885489.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            8972..9931
                     /gene="ftsN"
                     /locus_tag="ACHY09_002532"
     CDS             8972..9931
                     /gene="ftsN"
                     /locus_tag="ACHY09_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFS3885490.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKIAGHTNCIRLAAGG"
     gene            10024..10554
                     /gene="hslV"
                     /locus_tag="ACHY09_002533"
     CDS             10024..10554
                     /gene="hslV"
                     /locus_tag="ACHY09_002533"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFS3885491.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            10564..11895
                     /gene="hslU"
                     /locus_tag="ACHY09_002534"
     CDS             10564..11895
                     /gene="hslU"
                     /locus_tag="ACHY09_002534"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFS3885492.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            11962..12888
                     /gene="menA"
                     /locus_tag="ACHY09_002535"
     CDS             11962..12888
                     /gene="menA"
                     /locus_tag="ACHY09_002535"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS3885493.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            12981..13466
                     /gene="rraA"
                     /locus_tag="ACHY09_002536"
     CDS             12981..13466
                     /gene="rraA"
                     /locus_tag="ACHY09_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFS3885494.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(13551..13796)
                     /gene="zapB"
                     /locus_tag="ACHY09_002537"
     CDS             complement(13551..13796)
                     /gene="zapB"
                     /locus_tag="ACHY09_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFS3885495.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            14222..15067
                     /gene="glpF"
                     /locus_tag="ACHY09_002538"
     CDS             14222..15067
                     /gene="glpF"
                     /locus_tag="ACHY09_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFS3885496.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15090..16598
                     /gene="glpK"
                     /locus_tag="ACHY09_002539"
     CDS             15090..16598
                     /gene="glpK"
                     /locus_tag="ACHY09_002539"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS3885497.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            16769..17779
                     /gene="glpX"
                     /locus_tag="ACHY09_002540"
     CDS             16769..17779
                     /gene="glpX"
                     /locus_tag="ACHY09_002540"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFS3885498.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            17876..18622
                     /gene="fpr"
                     /locus_tag="ACHY09_002541"
     CDS             17876..18622
                     /gene="fpr"
                     /locus_tag="ACHY09_002541"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFS3885499.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(18627..19055)
                     /gene="uspD"
                     /locus_tag="ACHY09_002542"
     CDS             complement(18627..19055)
                     /gene="uspD"
                     /locus_tag="ACHY09_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFS3885500.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(19082..19381)
                     /locus_tag="ACHY09_002543"
     CDS             complement(19082..19381)
                     /locus_tag="ACHY09_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFS3885501.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
BASE COUNT         4689 a         5175 c         5201 g         4478 t
ORIGIN      
        1 ataaataact tcaccattca cactgtgaat tcatcgctca acacaaaagc ccccttatta
       61 ccggggcact gaccaaaggc aaaatgacaa tcaatcacca cctccgcagg aggtggttta
      121 gaggtgacaa caaaagctgg caactgccag cttttgttca ataatcacta caacaactgc
      181 gccagacggt taatatccga ctgaatcgcc ccggcggtga cgtcgcgccc tgcacctggc
      241 ccgcggatca ccaacggatt atcgcgatac cagcggcttt cgatggcaaa gacgttatcg
      301 cacggtagta gtgatgccag cggatggtct tctcgcaccg cttccacgcc tactcgcgct
      361 ttgccgttgg catcgaaacg cgccacgtag cgcaacacca gccccatttc acgggccgct
      421 tccaaccgtt gtaccatctg ctcattaagt tcatcgccat tttcaaagaa atggtcgatg
      481 cttccacctt cgcaatgggt tggcaccagt gattcaacac gcacctggtc cggttcaatg
      541 ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca tcacatcttt gccggagagg
      601 tcatcacgcg ggtcaggttc agttaagccc tgctgccacg cctgatccac cagctcagta
      661 aacggcacgc taccgtcgaa ttgcaggaac agccaggaga gcgtgccgga gaagatcccg
      721 ctgatcgaca aaatagtgtc gccgctgtcg atcagatcgc gcacggtatg gttgatcggc
      781 aaacctgcgc cgacggtggc gttgtacagc cagtgacgcc cggttttttc gaacgcgtcg
      841 tggatctggc gatatttatt gctgtcgctc gctcccgcca gtttgttggc actgatgacg
      901 tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa gctgctggct ggcagtaacg
      961 tccagcacaa ctaaatcatc atacggatgg gcacgcatcc acaggaatag cgactcttca
     1021 tcctgttcga cggcttcatc attaaagaag gctagcgcac ggctggcgtc cagcccgtca
     1081 tagctcaaca ggctgcgacg gctgtccacc acgcctgcca gcacaaattc aaagccggtg
     1141 cgcgccgaga gcgtgctctg ctcacgggcg aacagttcca gccagcggga accgatattt
     1201 cccttaccga acaataccag gccgatgcgt ttttctgcgc ggaagacgga ctgatgcagc
     1261 ccctgaatca ggctttcggt cgggccggtg cgcagtactg ccaccaggct gatgccgtca
     1321 tcggactgcc aggtaaactc gaccggctgg cctttcagtt gctgccagaa gcggtggcaa
     1381 tgcagcgggt tacgggtgac gcccgcccca accatcgcca ccagcgccag cccctgacgc
     1441 agacgcagtt cgccgggtaa tcctgcttcg tcgaggattt tcagcgcact gtcggccact
     1501 tccgaggtgt agcaaaattg cagcaactgg cgatcgttat gcacgccaac tgccagtggg
     1561 cgcacctgag cacgtttcag gatttggtcg atctctttat gcgccagttt aaaatcctga
     1621 ctggcgggca cctgaaactc aatcaaacag acatcatcgt ggctggtgac aatacgcgca
     1681 ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac cttgatccgg cgtgtagcta
     1741 cagcgcagct gcaggtcgat ttcactaccg gatactggct gtaaagtacg ggcgtgaaga
     1801 acgggggccg ccagacgcgc cagttcgctg gcttcatcaa gacgcagcaa tggcagcaga
     1861 caggcatctt tcactttacg cgggtcggca ctatataccc cggcgacatc gctccagatg
     1921 gttacgcggg aaacacccgc cagcgcaccg atttgtgtcg cggaatagtc ggaaccgtta
     1981 cgccccagca gcaccgtttc accggcgttg ttacggctga taaacccggt caccaccaga
     2041 cgtttgcccg gatgttgtac cagtagctgt tgcagcaacg ggtaagaaag cccttcatca
     2101 acctgcggct gcgcggcgcg ttcagcgcgt aaaaactcgc gggcatcaag ccaggccgct
     2161 ggcagccctt gttgattaag taccgcagac atcaggcgcg ccgaccacac ttcaccgtgg
     2221 cccaccactt ccgcatacac cgcatcatta ataccgctgt cgagcagcgc cgcgaggcgt
     2281 tccagatcgc tgacaaaggc gctaatgagg ctatcggctt cttcggcggg tagcaggccg
     2341 ctaatcagat cgcactgata acgacgtagc gtttgttgaa cctgatgcgc agagagacga
     2401 tcggtctggc ttagtttcaa ccagttaatc aactggttag tggtgctacc ggcggcggaa
     2461 accaccatca tatcgtcagg ctgagagtac tccgccataa tgcccgcgac acgcaaataa
     2521 cacttcacat cagccagact actgccacca aatttatgca gttgacgacc tttcgcccct
     2581 gcctgcgcaa tcacactcat ttttacccct tgtttgcagc ccggaagcca ttttccaggt
     2641 cggcaattaa atcttcgcca tcttcaatac cggtggagat acgtagcagc gtctcggaga
     2701 tcccggcggc agcacgcgct tctggcgcca tgcctgcatg tgtcatggtt gcggcgtgag
     2761 agattaaact ttccactccc cctaatgatt ccgccagcgt aaacaacgac aacccgccca
     2821 ggaaacgacg cagcgtctgc tcatcgccat ccagttcaaa actcaacatt gcgccaaagc
     2881 ctttttgctg gcgcgcggca atttcatgcc cctgattttc cggcagcgac gggtgataca
     2941 gttttttcac caacggctgg gtttgcagat atttcacaat cgcctgcgcg ttgcgctgcg
     3001 ccagctccat acgcggcacc agcgttcgca acccgcgcag cagcagatag ctgtcaaacg
     3061 cgccgcccgt cacgccaata ttgtttgccc accaggcaag ttcagtgaca acgtccgggt
     3121 ctttagcaat cactacgccg gccaccacgt ctgagtgacc gttcagatat ttcgtacatg
     3181 aatgcaacac cagatcggca cctaatgcca gcggattttg taatgccggg cttaagaagg
     3241 tgttatccac cacgctcacc gccccgactt cccttgccag atggcagatt ttcgcaatat
     3301 ccacgacgcg taacaatgga ttacttgggc tttctaccag taccagtttg ggtttttctg
     3361 ccagcgctac ccgtaatgcc tgttcatcgc cttgatcaac aaacaacacg cgatagcaac
     3421 cgcgtttcgc cagactgtcg aacaggcgat agctgccgcc atagcagtcg tgcggcgcaa
     3481 ccagcagatc gcccggtttc aaaaagacgg tcgttaccag atgaatcgcg gacatgccgg
     3541 tattagtaag tacggcacca gcaccgcctt ccagttctgc cagcgcacgc tgaaccacat
     3601 cgcgcgttgg gttgccgcgc cgcgagtaat catgcgcgcg cggttcatta aatccggtaa
     3661 agttataggt gctggaaaga tggatcggtg ggacaacgca accatactgt tcgtcgtcat
     3721 ttaacccgct acgcactgcg atggtggcct gtttacgcgt catgtgatga agttccctgg
     3781 gcttggtcgg tgaaatgtca ggcaccagag taaacattgt gttaatggac gtcaatacat
     3841 ctggacatct aaacttcttt gcgtatagat tgagcaaatc ccaaatagcc gttaaaatta
     3901 tatgcattat cacgccgaca ggtgcattac acgatgtcac ggtagcgcct gtacggtaaa
     3961 ctatgcgggt ttacggtcag tacccacata aattgtgtgg tctggtctca atttattgac
     4021 gaagaggatt aagtatctca tggctgaatg gagcggcgaa tatatcagcc catacgctga
     4081 gcacggcaag aagagtgaac aagtcaaaaa gattacggtt tccattcctc ttaaggtgtt
     4141 aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac aacctgcgtc acgctaccaa
     4201 cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc gggcaacctt tgccggatga
     4261 tgccgacctg cgtaaagagc gcagcgacga aatcccggaa gcggcaaaag agatcatgcg
     4321 tgagatgggg attaacccgg agacgtggga atactaaagc ggaaaagagc ggcgcggagt
     4381 taaccagccg cttttttcat acatggacgt ttaacgatga aaatcaggct actgatcctc
     4441 agcctgctgg tttctgtgcc tgcctttgcc tggcagccgc aaactggcga catcatcttt
     4501 caaatctctc gctcatcgca aagtaaagcg atccaactgg cgacccattc cgattacagc
     4561 cacaccggta tgctggtgat acgcaacaaa aagccctacg tttttgaagc ggtcggcccg
     4621 gtaaaataca ccccactcaa gcagtggatc gcccatggtg aaaagggcaa atacgttgtt
     4681 cgccgcgttg aaggcgaact gagtgttgaa caacagcaaa aactggcgca aacggcaaaa
     4741 cgttatctcg gtaaaccgta tgactccagc ttctcgtgga gcgacgatcg ccagtactgt
     4801 tcggaagtgg tttggaaggt ttatcagaac gcgctgggaa tgcgcgtggg caaacagcaa
     4861 aagttgaaag agttcgacct tagtaacccg caggttcagg caaagctcaa agaacgctac
     4921 ggcaaaaata ttccgcttga ggaaacggtg gtctcgccac aagccgtttt cgatgcgcca
     4981 caactcacca cggtcgccaa agaatggccg ctgttttcgt ggtaatacga ccaaaattca
     5041 ggcacaaaaa aagcgccgtg cggcgctttt ttcggaaatc cggtcttatt tgctgcccgg
     5101 gatgttgaaa cgcttgttga agcggtcaac acggccaccg gtagcaacat cacgctgttt
     5161 gccagtgaag aacgggtggc acttgctgca cacgtcgagg ttcaggtcat gaccaacggt
     5221 ggagcggatt ttcattacgt taccgcaaga gcagctagca gtaatttctt cgtatttcgg
     5281 gtgaatatct tttttcatgg gaaaacctcg gtttaaggcc gcgtcgctct tccagcccta
     5341 acgccagaca ccacgcgatg ttaaaagtat agcttcaata cgatcatttc gtatgaaggc
     5401 gcgaaatcat acagaaatta accagcgtat gcaaactgat ccgcactctt ctacggcaat
     5461 gtgtatacta acccaccgaa tttcaagtca ggatgatgct atgcccgttg cccacgttgc
     5521 cttgcccgtt ccgcttcccc gtacctttga ctatctgctg ccagaaggca tgacggtcaa
     5581 agccgggtgt cgcgtgcgcg tgccgtttgg caaacagcag gagcgcatcg gggttgtggt
     5641 gtcagttagc aatgtcagcg agctgccgct taacgagctg aaagcggtag ttgaagtgct
     5701 ggacgttgag ccggtattta ctcactccgt ctggcgatta ctgctctggg cggcagatta
     5761 ctatcatcat ccgattggcg atgtgctgtt tcatgccttg ccgattttac tacgccaggg
     5821 gcggcctgcg gcgaacgcgc cgatgtggta ctggtttgcc actgaacaag gtcaggcggt
     5881 ggatctgaac agcctgaaac gctcacccaa gcaacaacag gcgctggcag cgttacggca
     5941 aggcaaaatc tggcgcgacc aggttgccac gctcgaattt aatgatgccg cgttgcaggc
     6001 gctgcgcaaa aaaggtctgt gtgatttagc aagtgaaaca ccagagttta gtgactggcg
     6061 aaagaattat gccgtttctg gtgagcggtt gcgattgaat actgaacagg ccaccgccgt
     6121 tggcgcaatt catagtgcgg cggatacctt ctctgcctgg ctgctggcgg gcgttaccgg
     6181 ttccggtaaa acggaggttt atctcagtgt actggaaaac gtgctcgctc agggcaaaca
     6241 ggcgctggtg atggtgccgg aaatcggcct gacgccgcaa actatcgccc gttttcgtga
     6301 acgttttaat gcgccagtgg aagttctgca ttccggcctg aacgacagcg agcgtctttc
     6361 ggcgtggttg aaagcgaaaa atggtgaggc ggcgattgtg atcggcaccc gctccgcgct
     6421 gtttacgccg tttaaaaatc tcggtgtgat tgtcattgat gaagagcacg acagttccta
     6481 caagcagcag gaaggctggc gctatcatgc ccgcgacctg gcggtttatc gtgcgcacag
     6541 cgagcaaatc ccgattattc ttggctccgc aacacccgca ctggaaacgt tatgcaacgt
     6601 ccagcagaaa aaataccgcc tgctgcgcct gacgcgtcgg gcggggaatg cgcgtccggc
     6661 aattcaacac gtgctggatt taaaaggtca gaaggtgcag gcggggctgg ctccggcgtt
     6721 aatcacccgt atgcgccagc atttacaggc cgacaaccag gttatcctct ttcttaaccg
     6781 ccgtggcttt gcgcctgcct tgctgtgcca cgactgcggc tggattgccg aatgtccacg
     6841 ttgcgatcac tactacacgc tgcatcaggc gcaacagcat ttgcgctgtc accactgtga
     6901 cagccaacgc ccggtgccgc gccagtgccc ttcctgcggt tccacgcacc tggtgccggt
     6961 ggggctgggc accgaacagc ttgaacagac gcttgcgccg ctgttccctg gtgtgcccat
     7021 ttctcgtatc gaccgcgata ccaccagccg caaaggagcg ctggaacaac aactggcgga
     7081 agtgcatcgc ggcggcgcga ggatcttgat cggcacgcaa atgctggcga aaggtcacca
     7141 ctttccggat gtgacgctgg tggcgttact ggacgtagac ggcgcgctgt tttctgccga
     7201 ttttcgctcg gcagagcgtt tcgcccagct ttacacccag gtcgccggtc gtgccgggcg
     7261 tgcgggtaaa cagggcgaag tggtgctgca aacgcatcat ccagaacacc cgctattgca
     7321 aacgttgctc tataaaggct atgacgcctt tgccgaacag gcactggctg agcgacgcat
     7381 gatgcagctg ccgccgtgga ccagccatgt gattgtgcgt gcggaagatc ataacaatca
     7441 gcacgcgccg ctgttcctgc aacaactgcg taatctcatc ctctccagcc cgctggcaga
     7501 cgacaaactg tgggttctcg gtcccgttcc ggcactggca cctaaacgtg gcggccgctg
     7561 gcgctggcaa attttgttgc agcacccttc ccgcgtgcga ttacagcaca tcatcaatgg
     7621 tacgctggcg cttattaata caataccgga ttcccgtaag gtgaaatggg tgctggatgt
     7681 cgatcccatc gagggctaat gcgctcacga tgcgaggcgg atcgaaaaat tcaatattca
     7741 tcacactttt catgaaaatt ctgtaaccgt tttcacgcgc tatctgctaa aaatgttgcc
     7801 gatgtgaagt aaacatggat gtagtacgcc tgacgtgcca ggcgaggagt gagtgtgaaa
     7861 gcgaagaagc aggaaactgc cgcgaccatg aaagacgttg ccctcaaggc aaaagtctct
     7921 acagcgactg tctcccgagc attaatgaat cccgataaag tctcccaggc cacccgtaat
     7981 cgggttgaaa aagcggcccg ggaagtgggt tatttaccgc agcctatggg gcgcaacgtc
     8041 aagcgtaatg aatcccgcac tattctggtg attgtcccgg atatctgcga tcccttcttt
     8101 agcgaaatta ttcgcggtat cgaagttacg gcggcaaatc acggatatct ggtgctgatt
     8161 ggcgactgtg cgcatcaaaa tcagcaggaa aaaaccttta tcgatttgat catcaccaag
     8221 caaattgatg gcatgttgct gctgggttca aggctgccgt ttgatgccag cattgaggaa
     8281 cagcgtaatc tgccgccgat ggtgatggcg aacgaatttg caccggaact ggagctgcct
     8341 accgttcata tcgacaatct gaccgccgca tttgatgcag taaattattt atatgagcaa
     8401 gggcataatc ggattggctg tatagccggt cccgaagaga tgccgctgtg tcactaccgc
     8461 ctgcaaggct atgtccaggc gctgcgtcgc tgcggcatta tggttgatcc gcaatacatc
     8521 gcccgtggcg acttcacctt cgaagccgga agcaaagcga tgcagcagtt gcttgatctt
     8581 ccacaaccgc ctactgctgt cttctgccat agcgatgtga tggcgctcgg cgcactttct
     8641 caggcaaaac gccaggggct gaaagtcccg gaagaccttt ccataatcgg ttttgataac
     8701 atcgacctga cgcaattttg tgatccgccg ctgacaacca tcgcgcagcc gcgttacgaa
     8761 atcggtcggg aagctatgct gttattgctt gatcaaatgc aggggcaaca cgttggcagt
     8821 ggctctcgtt taatggactg cgaacttatc atccggggat caacacgcgc gttaccttaa
     8881 agtaaacctt taagactgcc gtgactggtc aaaggcccgc cgcttaagta acatggcggg
     8941 ctgacgaacg aataaataca gcgaaacgat agtggcacaa cgagattatg tacgccgcag
     9001 ccaaccggca ccttcgcggc gaaaaaagag cacctcacgg aaaaagcaac gaaatctgcc
     9061 tgcggtttct cccgctatgg tcgctattgc tgccgccgtt cttgtgacct ttatcggtgg
     9121 tctgtacttc attacgcatc acaagaaaga agagtccgag acactgcaaa gccagaaagt
     9181 gaccggaaac ggactaccac caaaaccaga agaacgctgg cgctacatca aagagctgga
     9241 aagtcgccag ccgggtgtgc gtgcgcctac agaaccttct gccggtggtg aagtgaaaac
     9301 gccggagcaa ctgacaccag aacaacgcca gcttcttgag caaatgcagg ctgatatgcg
     9361 ccagcagcca acgcagctgg ttgaagttcc gtggaatgaa cagacgccgg aacagcgtca
     9421 gcaaacgcta cagcgccagc gtcaggcgca gcaattagcg gagcagcaac gcctggctca
     9481 gcagtcgcgt acgactgaac aaagctggca gcagcagacg cgtacgtcgc aagccgcgcc
     9541 ggtacaggcg caaccgcgcc agtctaaacc ggcttctact cagcagccgt accaggatct
     9601 gctgcaaacc cctgcgcaca cgaccgcgca gtcgaagccg cagcaagctg cgccggttgc
     9661 tcgtgccgct gacgcgccaa aaccgacggc ggagaaaaaa gacgaacgcc gctggatggt
     9721 gcagtgcggt tcgttcagag gcgcggaaca ggcagagacg gtacgtgctc agctggcgtt
     9781 cgaaggcttt gactcaaaaa ttaccaccaa caatggctgg aatcgtgtgg tgattggccc
     9841 ggtgaaaggc aaagagaacg cagacagcac cctcaatcgc ttgaagatag cgggtcatac
     9901 aaactgcatt cggctcgccg ccgggggttg aaaccctcaa aatccccccc atctataatt
     9961 gcattatgcc ccgtactttt gtacggggtt tgtactctgt attcgtaacc aaggggtcag
    10021 ctcgtgacaa ctatagtaag cgtacgccgt aacggccatg tggtcatcgc tggtgatggt
    10081 caggccacgt tgggcaatac cgtaatgaaa ggcaacgtga aaaaggtccg ccgtctgtac
    10141 aacgacaaag tcatcgcggg ctttgcgggc ggtactgcgg atgcttttac gctgttcgaa
    10201 ctgtttgaac gtaaactgga aatgcatcag ggccatctgg tcaaagccgc cgttgagctg
    10261 gcgaaagact ggcgtaccga tcgcatgctg cgcaaacttg aagcattgct ggcagtcgcg
    10321 gatgaaactg catcgcttat catcaccggt aacggtgacg tggtgcagcc agaaaacgat
    10381 cttattgcta tcggctccgg cggcccttac gcccaggctg cggcgcgcgc gctgttagaa
    10441 aacactgaac ttagcgcccg tgaaattgct gaaaaggcgt tggatattgc aggcgacatt
    10501 tgcatctata ccaaccattt ccacaccatc gaagaattaa gctacaaagc gtaaggatct
    10561 cccatgtctg aaatgacccc acgcgaaatc gtcagcgaac tggataagca catcatcggc
    10621 caggacaacg ccaagcgttc cgtggcgatt gctctgcgta accgctggcg tcgcatgcag
    10681 ctcaacgaag agctgcgcca tgaagtgacc ccgaaaaata tcctgatgat cggcccgacc
    10741 ggtgtcggta aaactgaaat cgcccgtcgc ctggctaagc tggcgaatgc gccgttcatc
    10801 aaagttgaag cgaccaaatt caccgaagtg ggctatgtcg gtaaggaagt ggattctatt
    10861 attcgcgatc tgaccgatgc cgccgtgaaa atggtacgcg tccaggctat cgagaaaaac
    10921 cgttatcgcg ctgaagaact ggcagaagaa cgtattctcg acgtactgat cccaccggct
    10981 aaaaataact ggggacagac cgaacagcag caggaaccgt ccgctgctcg tcaggcattc
    11041 cgcaaaaaac tgcgtgaagg ccagcttgat gacaaagaaa tcgagatcga tcttgccgca
    11101 gcaccgatgg gcgttgaaat tatggctcct ccgggcatgg aagagatgac cagccagctg
    11161 cagtccatgt tccagaacct gggcggtcag aaacaaaaag cgcgtaagct gaaaatcaaa
    11221 gacgccatga agctgctgat tgaagaagaa gcggcgaaac tggtgaaccc ggaagagctg
    11281 aagcaagatg ctatcgacgc tgttgagcag cacgggatcg tgtttatcga cgaaatcgac
    11341 aaaatctgta agcgcggcga gtcttccggt ccggatgttt ctcgtgaagg cgttcagcgt
    11401 gacctgctgc cgctggtaga aggttgcact gtttccacca aacatgggat ggtcaaaact
    11461 gaccacattc tgtttatcgc ttctggcgcg ttccagattg cgaaaccgtc tgacctgatc
    11521 ccggaactgc aaggtcgtct gccaatccgc gttgagctgc aggcactgac caccagcgac
    11581 ttcgagcgta ttctgaccga gccgaatgcc tctatcaccg tgcagtacaa agcactgatg
    11641 gcaactgaag gcgtaaatat cgagtttacc gactccggta ttaagcgcat cgcggaagcg
    11701 gcatggcagg tgaacgaatc taccgaaaac atcggtgctc gtcgtttaca taccgttctg
    11761 gagcgtttaa tggaagagat ttcctacgac gccagcgatt taagcggtca aactatcact
    11821 attgacgcag attatgtgag caaacatctg gatgcgttgg tggcagatga agatctgagc
    11881 cgttttatcc tataatcgcg ttcaatcatt ttcatcattg tttgatgggg ctgaaaggcc
    11941 ccatttttat tggcgcgtat tatgactgaa caacaaatta gccgaactca ggcgtggctg
    12001 gaaagtttac gacctaaaac cctccccctc gcctttgctg caattatcgt cgggacggcg
    12061 ctggcatggt ggcaaggtca cttcgatcca ctggtcgccc tgctggcatt gattaccgcc
    12121 gggctattac agatcctttc taacctcgcc aatgattacg gcgatgcggt aaaaggcagc
    12181 gataaacctg accgcattgg gccgctacgc ggcatgcaaa aaggggtcat tacccagcaa
    12241 gagatgaaac gggcgctcat tattaccgtt gtgctcatct gtctttccgg gctggcactg
    12301 gttgcagtgg cgtgccatac gctggccgat tttgtcggtt tcctgattct tggcgggttg
    12361 tcgatcattg ccgctatcac ctacaccgtg ggcaatcgtc cttatggtta tatcggtttg
    12421 ggtgatattt ccgtactggt tttctttggc tggttgagcg tcatggggag ctggtattta
    12481 caggctcata cattgattcc ggcactgatc cttccggcaa ccgcatgcgg cctgctggca
    12541 acggcagtac tgaacattaa taacctgcgt gatatcaata gcgaccgcga aaacggcaaa
    12601 aacacgctgg tggtgcgctt aggtgcagta aacgcgcgtc gttatcatgc ctgcctgctg
    12661 atgggctcac tggtgtgtct ggcgctgttt aatctctttt cgctgcatag cctgtggggc
    12721 tggctgttcc tgctggcggc accattactg gtgaagcaag cccgttatgt gatgcgggaa
    12781 atggacccgg tggcgatgcg accgatgctg gaacgcactg tcaagggagc gttactgact
    12841 aacctgctgt ttgttttagg gatattccta agccagtggg cagcataact gacaaatatc
    12901 aattaacaat tgatgatttt gccaacagcc tacatagcgc gatatactga aaattctcgc
    12961 agcaactgaa tgttaagcct atgaaatacg atacttccga gctttgtgac atctatcaag
    13021 aagatgttaa cgtcgtggaa ccgctgttct ccaactttgg cggacgggcg tcgtttggcg
    13081 gacaaataat cacggtaaaa tgtttcgagg acaacgggtt gctgtacgat ctgctcgaac
    13141 agaatggccg tggtcgtgtt cttgtcgtcg atggcggtgg ttctgttcgt cgcgcactgg
    13201 tcgatgctga actggcgcgt ctggcagtac aaaatgaatg ggaaggtctg gtcatttacg
    13261 gcgcggtgcg tcaggtagat gacctggaag agctggatat cggcatccag gcaatggccg
    13321 caattccggt tggtgccgct ggcgaaggca ttggcgaaag cgatgtccgc gtcaattttg
    13381 gcggtgtaac cttcttctcc ggcgaccatc tttacgccga caataccggg attattcttt
    13441 cagaagatcc gctggatatt gaatgataaa aaaggcaccg caaggtgcct tttttctgcg
    13501 ttacctgttg gcctacacag taaagaaatt acgcgaaaga tgaagcgtaa tcagacctct
    13561 tccatgcgac ccagcagggc ctgcagacgt tcctgccagc cgttctgctg ttctttcaga
    13621 tggttgttct cacgctccag ctcttcgcgc tgatgctggg cattttgaac ttcctgcgac
    13681 agtgagttgt ttttttcttt cagctcttcg atttccatct gcaacagagt gatggtatca
    13741 atcgcctgct gtacttttgc ttccagtttc tcaaacactt ctaatgacat tgtcatacct
    13801 ctcctgaatt gcaaggcgtt gatggataaa aaatcctcgt cccgattacc ggtgacgcct
    13861 taataaatac gagcgcactt tagttagctc cgattgtatg aagccgcgcc atcgctgtcc
    13921 agcggcacgc cttgcagatt acggtttgcc acacttttca tccttctcct ggagacataa
    13981 tccacaccaa tcgaaaatgt taataaattt gttgcgcgaa tgatctaaca aacatgcatc
    14041 atgtacaatc agatggaata aatggcgcga taacgctcat tttatgacga agcacacaca
    14101 ttttaagttc gatatttctc gtttttgctc gttaacgata agtttacagc atgcctacaa
    14161 gcatcgtgga ggtccgtgac tttcacgcat acaacaaaca ttaactcttc aggatccgat
    14221 tatgagtcaa acatcaacct tgaaaggcca gtgcattgct gaattcctcg gtaccgggtt
    14281 gttgattttt ttcggtgtgg gttgcgttgc agcactaaaa gtcgctggtg cgtcttttgg
    14341 tcagtgggaa atcagtgtca tttggggact gggggtggca atggccatct acctgaccgc
    14401 aggggtttcc ggcgcgcatc ttaaccccgc tgttaccatt gcattgtggc tgtttgcctg
    14461 tttcgacaag cgcaaagtta tcccttttat cgtttcacaa gttgccggcg ctttctgtgc
    14521 tgcggcttta gtttacgggc tttactacaa tttatttttc gacttcgagc agactcatca
    14581 tattgttcgc ggcagtgttg aaagtgttga tctggctggc actttctcta cttaccctaa
    14641 tcctcatatc aattttgtgc aggctttcgc agttgagatg gtgattaccg ctattctgat
    14701 ggggctgatc ctggcgttaa cggacgatgg caacggtgta ccacgcggcc ctttggctcc
    14761 cttgctgatt ggtctactga ttgcggtcat tggcgcatct atgggcccat tgacgggttt
    14821 tgccatgaac ccagcgcgtg acttcggtcc gaaagtcttt gcctggctgg cgggctgggg
    14881 caatgtcgcc tttaccggcg gcagagacat tccttacttc ctggtgccgc tttttggccc
    14941 tatcgttggc gcgattgtag gtgcatttgc ctaccgcaaa ctgattggtc gccatttgcc
    15001 ttgcgatatc tgtgttgtgg aagaaaagga gaccacaact ccttcagaac aaaaagcttc
    15061 gctgtaatat gactacggga caattaaaca tgactgaaaa aaaatatatc gttgcgctcg
    15121 accagggcac caccagctcc cgcgcggtcg taatggatca cgatgccaat atcattagcg
    15181 tgtcgcagcg cgaatttgag caaatctacc caaaaccagg ctgggtagaa cacgacccaa
    15241 tggaaatctg ggccacccaa agctccacgc tggtagaagt gctggcgaaa gccgatatca
    15301 gttccgatca aattgcagct atcggtatta cgaaccagcg tgaaaccact attgtctggg
    15361 aaaaagaaac cggcaagcct atctataacg ccattgtctg gcagtgccgt cgtaccgcag
    15421 aaatctgcga gcatttaaaa cgtgacggtt tagaagatta tatccgcagc aataccggtc
    15481 tggtgattga cccgtacttc tctggcacca aagtgaagtg gatcctcgac catgtggaag
    15541 gctctcgcga gcgtgcgcgt cgtggtgaat tgctgtttgg tacggttgat acgtggctta
    15601 tctggaaaat gactcagggc cgtgtccatg tgaccgatta caccaacgcc tctcgtacca
    15661 tgttgttcaa catccatacc ctggactggg acgacaaaat gctggaagtg ctggatattc
    15721 cgcgcgagat gctgccagaa gtgcgtcgtt cttccgaagt gtacggtcag actaacattg
    15781 gcggcaaagg cggcacgcgt attccaatct ccgggatcgc cggtgaccag caggcggcgc
    15841 tgtttggtca gttgtgcgtg aaagaaggaa tggcgaagaa cacctatggc actggctgct
    15901 ttatgctgat gaacactggc gagaaagcgg tgaaatcaga aaacggcctg ctgaccacca
    15961 tcgcctgcgg cccgactggc gaagtaaact atgcgctgga aggtgcggtg tttatggcgg
    16021 gcgcgtccat tcagtggctg cgcgatgaga tgaagttgat taacgacgcc tacgattccg
    16081 aatatttcgc caccaaagtg caaaacacca atggtgtgta tgtggttccg gcctttaccg
    16141 ggctgggtgc gccatactgg gatccgtatg cacgcggggc gattttcggt ctgactcgtg
    16201 gggtgaacgc taaccacatt atacgcgcga cgctggagtc tattgcttat cagacgcgtg
    16261 acgtgctgga agcaatgcag gccgactccg gtattcgtct gcacgcgctg cgcgtggacg
    16321 gcggtgcggt ggcgaacaat ttcctgatgc agttccagtc cgatattctc ggtacccgcg
    16381 ttgagcgccc ggaagtgcgc gaagtcaccg ctttgggtgc ggcctatctt gctggtctgg
    16441 cggttggctt ctggcagaat cttgacgagc tgcaagagaa agcggtgatt gaacgcgagt
    16501 tccgtccagg catcgaaacc actgagcgta attaccgtta cgcaggctgg aaaaaagcgg
    16561 tcaaacgcgc gatggcatgg gaagaacacg acgagtaata atgattgcct gatgcgacgc
    16621 ttgcgcatct tatcaggcct acgagtcgga tacacatccg gcatcacgca gactctgaaa
    16681 ccgtgcgggg gcaaccccgc acacatcaat aatcccgccc ttcccctgtg ctacacttcg
    16741 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    16801 cgtcaccgaa tcagcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    16861 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca acattgacgg
    16921 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    16981 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    17041 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    17101 tttcctcaac gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    17161 caccattgat ctgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    17221 caaaccgttg agcgaactga cggtaacgat tctggcaaaa ccgcgccacg atgccgttat
    17281 cgcagaaatg cagcaactcg gcgtgcgcgt atttgctatt ccggacggcg acgttgcagc
    17341 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    17401 gccggaaggc gtggtttctg cggcggtgat ccgcgcatta gatggtgaca tgaacggtcg
    17461 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    17521 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gtttgggcga
    17581 tatggcgcgc agcgacaacg tcatcttttc tgccaccggc atcaccaaag gtgatctgct
    17641 ggaaggtatt agccgcaaag gcaatatcgc caccaccgaa acgctgctga tccgtggtaa
    17701 atcacgcacc attcgtcgca ttcagtccat ccactatctg gatcgtaaag acccggaaat
    17761 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    17821 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    17881 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    17941 caccgttcac gctcccgtgc ttccgtttac cgccgggcaa ttcaccaaac ttggccttga
    18001 aattgaaggc gaacgcgttc agcgcgccta ctcctatgtt aactcgcccg ataatccgga
    18061 tctggagttt tacctggtca ccgtccccga tggcaaatta agtccacgac tggcggcact
    18121 gaaaccgggc gatgaagtgc aggtggttag cgaagccgct ggcttctttg tgctggatga
    18181 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    18241 atcgattctg caattgggca aagatttaga acgcttcaaa aacatggtgc tggttcacgc
    18301 cgcacgatat gccgccgact taagctatct acctctgatg caggaactgg aaaaacgcta
    18361 cgaggggaaa ctgcgcattc agacggtggt cagtcgggaa acggctgcgg gttcgctcac
    18421 tgggcgatta ccggcgttaa ttgaaagtgg tgaactggaa agcgcggttg gcctgccgat
    18481 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    18541 acagttgctg aaggagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    18601 gacagcggag cattactggt aagcggttac ttgtcgataa acggcacgat caagagatcc
    18661 gcactcaact tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtga
    18721 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagcgtttcc
    18781 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    18841 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    18901 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    18961 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    19021 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    19081 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaagg
    19141 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    19201 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    19261 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    19321 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    19381 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    19441 aaggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    19501 tgaagagttc agaaggattt tccgggcatt gtcacttgca tcg
//
LOCUS       DAZUCC010000084        19508 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZUCC010000084 DAZUCC010000000
VERSION     DAZUCC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.084"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            226..561
                     /gene="yjdM"
                     /locus_tag="ACHY09_002544"
     CDS             226..561
                     /gene="yjdM"
                     /locus_tag="ACHY09_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS3885502.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            721..1164
                     /gene="yjdN"
                     /locus_tag="ACHY09_002545"
     CDS             721..1164
                     /gene="yjdN"
                     /locus_tag="ACHY09_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFS3885503.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            1297..2085
                     /gene="phnC"
                     /locus_tag="ACHY09_002546"
     CDS             1297..2085
                     /gene="phnC"
                     /locus_tag="ACHY09_002546"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFS3885504.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2110..3126
                     /gene="phnD"
                     /locus_tag="ACHY09_002547"
     CDS             2110..3126
                     /gene="phnD"
                     /locus_tag="ACHY09_002547"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3885505.1"
                     /translation="MNAKIIASLTFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3232..4011
                     /gene="phnE"
                     /locus_tag="ACHY09_002548"
     CDS             3232..4011
                     /gene="phnE"
                     /locus_tag="ACHY09_002548"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS3885506.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4032..4757
                     /gene="phnF"
                     /locus_tag="ACHY09_002549"
     CDS             4032..4757
                     /gene="phnF"
                     /locus_tag="ACHY09_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFS3885507.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     HFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4758..5210
                     /gene="phnG"
                     /locus_tag="ACHY09_002550"
     CDS             4758..5210
                     /gene="phnG"
                     /locus_tag="ACHY09_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFS3885508.1"
                     /translation="MHADTATRQHWMSVLAHSQPTELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLNADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5207..5791
                     /gene="phnH"
                     /locus_tag="ACHY09_002551"
     CDS             5207..5791
                     /gene="phnH"
                     /locus_tag="ACHY09_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFS3885509.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5791..6855
                     /gene="phnI"
                     /locus_tag="ACHY09_002552"
     CDS             5791..6855
                     /gene="phnI"
                     /locus_tag="ACHY09_002552"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFS3885510.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVQEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6848..7693
                     /gene="phnJ"
                     /locus_tag="ACHY09_002553"
     CDS             6848..7693
                     /gene="phnJ"
                     /locus_tag="ACHY09_002553"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFS3885511.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMNMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7690..8448
                     /gene="phnK"
                     /locus_tag="ACHY09_002554"
     CDS             7690..8448
                     /gene="phnK"
                     /locus_tag="ACHY09_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFS3885512.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8559..9239
                     /gene="phnL"
                     /locus_tag="ACHY09_002555"
     CDS             8559..9239
                     /gene="phnL"
                     /locus_tag="ACHY09_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFS3885513.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGVSS"
     gene            9236..10372
                     /gene="phnM"
                     /locus_tag="ACHY09_002556"
     CDS             9236..10372
                     /gene="phnM"
                     /locus_tag="ACHY09_002556"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFS3885514.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGKIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTEAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10372..10929
                     /gene="phnN"
                     /locus_tag="ACHY09_002557"
     CDS             10372..10929
                     /gene="phnN"
                     /locus_tag="ACHY09_002557"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFS3885515.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10916..11350
                     /gene="phnO"
                     /locus_tag="ACHY09_002558"
     CDS             10916..11350
                     /gene="phnO"
                     /locus_tag="ACHY09_002558"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFS3885516.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYQLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            11352..12110
                     /gene="phnP"
                     /locus_tag="ACHY09_002559"
     CDS             11352..12110
                     /gene="phnP"
                     /locus_tag="ACHY09_002559"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFS3885517.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACVRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHSGLLDFSHTVEPFVMFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVA"
     gene            complement(12138..12467)
                     /gene="yjdP"
                     /locus_tag="ACHY09_002560"
     CDS             complement(12138..12467)
                     /gene="yjdP"
                     /locus_tag="ACHY09_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFS3885518.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12536..12985)
                     /gene="rpiB"
                     /locus_tag="ACHY09_002561"
                     /gene_synonym="alsI"
     CDS             complement(12536..12985)
                     /gene="rpiB"
                     /locus_tag="ACHY09_002561"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFS3885519.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASHVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            13344..14234
                     /gene="alsR"
                     /locus_tag="ACHY09_002562"
     CDS             13344..14234
                     /gene="alsR"
                     /locus_tag="ACHY09_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFS3885520.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14293..15228
                     /gene="alsB"
                     /locus_tag="ACHY09_002563"
     CDS             14293..15228
                     /gene="alsB"
                     /locus_tag="ACHY09_002563"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFS3885521.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15356..16888
                     /gene="alsA"
                     /locus_tag="ACHY09_002564"
     CDS             15356..16888
                     /gene="alsA"
                     /locus_tag="ACHY09_002564"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFS3885522.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAEAQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCHPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     HE"
     gene            16867..17847
                     /gene="alsC"
                     /locus_tag="ACHY09_002565"
     CDS             16867..17847
                     /gene="alsC"
                     /locus_tag="ACHY09_002565"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFS3885523.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17858..18553
                     /gene="alsE"
                     /locus_tag="ACHY09_002566"
     CDS             17858..18553
                     /gene="alsE"
                     /locus_tag="ACHY09_002566"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFS3885524.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDNHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            18537..19466
                     /gene="alsK"
                     /locus_tag="ACHY09_002567"
     CDS             18537..19466
                     /gene="alsK"
                     /locus_tag="ACHY09_002567"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFS3885525.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
BASE COUNT         4591 a         5439 c         5288 g         4190 t
ORIGIN      
        1 atgtgaatta aaaaaagaac gagtagagca ttaaccttat cttaaataat aaatggaggc
       61 aaaaagatta ttttcttttt gcgtttcctt tcaaatgaaa acgatcgtcg tctaaaatca
      121 gcagtacccc cgacaaactc agggattttg tgtataattg cggccttttt cggcaatctg
      181 ccgttttttg gcgcttttgc cctgctgact tttgaggaaa tccacatgtc attaccacac
      241 tgcccaaaat gcaactccga atacacctac gaagataacg gcatgtacat ctgcccggaa
      301 tgtgcctacg aatggaacga cgcagaacct gcacaggaaa gcgacgagct gatcgttaaa
      361 gatgctaacg gcaatctgct ggctgacggc gacagcgtta ccatcattaa agatctgaaa
      421 gtgaaaggta gctcttcgat gctgaaaatt ggcaccaaag tgaaaaacat ccgcctggtt
      481 gaaggcgacc ataacatcga ttgcaaaatc gacggttttg gtccgatgaa actgaagtct
      541 gagtttgtga aaaagaactg attgtattgt gatcggtagg ccggataagg cgtttacgcc
      601 gcatccggca acggtggcga ctgcctgatg cgacgctgac cgcgtcttat caggcttccc
      661 tctcatctgt ataaatttcg aactacactt aacgggcttc tcttaactga ggtcaccatc
      721 atgccgttaa gtccctacct ctcttttgcc ggtaactgtt ccgacgcgat tgcctattat
      781 caacgtacgt tgggcgcgga actgctctat aaaatcagct tcggcgaaat gccgaaatca
      841 gcgcaggaca gcgccgagaa ctgcccttcc ggaatgcaat ttcccgatac cgccatcgct
      901 catgccaacg tgcgcattgc aggaagcgac atcatgatga gcgatgctat accgtcagga
      961 aaagccagct actccggctt tacgctagtg ctcgattcgc aacaggtcga agaaggaaaa
     1021 cgctggtttg ataaccttgc agctaacgga aaaatcgaaa tggcctggca ggaaactttc
     1081 tgggcgcatg gcttcggcaa agtcaccgat aaatttggcg taccgtggat gattaatgtc
     1141 gtcaaacaac aaccaacgca ctaacccgcc gggagggccg ccctcccgca ctgtcatcga
     1201 aatctcgtta actcttcatc cgttagtcac ttttagttaa caaaatcgtc acaataatcc
     1261 gccacgatgg agccactttt ttagggaggc tgcatcatgc aaacgattat ccgtgtcgag
     1321 aagctcgcca aaaccttcaa tcagcatcag gcgctgcacg cggttgatct gaacattcat
     1381 cacggtgaaa tggtggctct gcttgggccg tcgggttccg gcaaatccac ccttttacgt
     1441 cacttaagcg gtttgattac cggcgataaa tctgccggta gccatatcga gctgctgggc
     1501 cgcacagtcc agcgcgaagg ccgcctggcc cgcgatatcc gcaaaagccg cgccaatacc
     1561 ggctacatct tccaacaatt caacctggtg aatcgcctga gcgtactgga gaacgtgctg
     1621 attggcgcgc tcggcagcac gccattctgg cgcacctgtt ttagctactt cacccgcgag
     1681 cagaaacagc gcgcgttaca ggcgctgacc cgcgttggca tggtgcactt tgcccatcag
     1741 cgcgtttcca ccctctccgg cggacagcaa cagcgtgtgg cgattgcccg cgcgctgatg
     1801 cagcaggcga aggtgattct ggcggacgaa cccatcgcct cgctggaccc ggaatcggcg
     1861 cgcatcgtga tggacaccct gcgcgacatt aaccagaacg acggcattac cgtggtcgtc
     1921 acgctgcatc aggtggatta cgccctgcgc tactgcgaac gcatcgtcgc cctgcgccag
     1981 gggcacgtct tctacgacgg cagcagccaa cagtttgata acgaacgttt tgaccatctc
     2041 taccgcagca tcaaccgcgt cgaagaaaac gcgaaagctg cctgacatcc ccatcattga
     2101 ggaaaacgaa tgaacgctaa gataattgcc tcgctgacct tcaccagcat gttcagcctc
     2161 agcaccctgt taagcccggc gcacgccgaa gagcaggaaa aggcgctgaa tttcggcatt
     2221 atttcaacgg aatcacagca aaacctgaaa ccgcaatgga cgccgttctt acaggatatg
     2281 gagaagaagc tgggcgtgaa ggtcaacgcc ttctttgccc cggactacgc gggcattatc
     2341 cagggaatgc gcttcaacaa agtggatatc gcctggtacg gcaatctgtc agcgatggaa
     2401 gcggtggatc gcgccaatgg ccaggtcttc gcccagacgg tcgcggcgga tggatcgccg
     2461 ggttactgga gcgtgttgat cgtcaacaaa gatagtccga tcaacaacct gaacgatctg
     2521 ctggccaagc gtaaagatct caccttcggc aatggcgatc ctaactccac ctcaggcttc
     2581 ctcgtccccg gctactacgt cttcgccaaa aacaatatct ccgccagcga cttcaagcgc
     2641 accgtcaacg ccgggcatga aaccaacgcg ctggccgttg ccaacaagca ggtggatgtt
     2701 gccaccaaca acaccgaaaa cctcgacaag ctgaaaacct ccgcaccgga gaagctcaaa
     2761 gaactgaagg tgatctggaa atcgccgctg atcccaggcg atccgatcgt ctggcgcaaa
     2821 aatctgtccg aaaccaccaa agacaagatc tacgacttct ttatgaacta cggcaaaacg
     2881 ccggaagaga aagcggtgct ggaacgcctg ggctgggccc cgttccgcgc ctccagcgac
     2941 ctgcaactgg tgccgattcg ccagctcgcg ctgtttaaag agatgcaggg cgtgaaaggc
     3001 aataaagggc tgaatgagca ggacaagctg gcaaaaacca gcgccattca ggcgcaactg
     3061 gatgacctgg accgcctgaa caacgcgtta agcgcgatga gttcggtgag taaagcggtg
     3121 cagtaaatcg taggtcggat aagacgcccc ggcgtcgcat ccgacaatgt gcagacgttg
     3181 atgccggatg cggtgcaagc accttatccg gcctacagac cggagccaaa catgcaaacc
     3241 atcaccatcg ccccacccaa gcgcagctgg ttctcgcttc tgagctgggc cattgttctc
     3301 gccgtgttgg tcgtctcgtg gcagggcgcg gaaatggccc cgcttacgct gatcaaagac
     3361 ggcggcaaca tggcaacctt cgccgccgac ttcttcccgc ccgatttcag ccagtggcag
     3421 gattacctca ccgaaatggc cgtcacgctg caaatcgccg tctggggcac cgcgctggcg
     3481 gtggttctct ccatcccctt cggtctgatg agcgccgaaa acctggtgcc gtggtgggtt
     3541 taccagcccg tacgccgcct gatggacgcc tgccgcgcca ttaacgaaat ggtcttcgcc
     3601 atgctgttcg tggtcgccgt cggccttggc ccgttcgctg gcgtgctggc gctgtttatc
     3661 cacaccaccg gcgtgctctc caagctgctt tccgaagcgg tagaagcgat cgaacccggc
     3721 ccggtggagg gcattcgcgc caccggtgcc aacaagctgg aagagatcct ctacggcgtg
     3781 ctgccacagg tgatgccgct gctgatctcc tactccctct atcgcttcga atccaacgtc
     3841 cgctcggcga ccgtcgtcgg catggtcggt gcaggcggga tcggcgtcac cctgtgggaa
     3901 gcgattcgcg gtttccagtt ccagcaaacc tgcgccctga tggtgctcat catcgtcacg
     3961 gtcagcctgc tggatttcct ctctcaacgg ttgcgtaagc actttatctg ataagcgagg
     4021 cattgatagc tatgcacttg tctacacatc cgaccagcta cccaacacgc tatcaggaga
     4081 tagccgcaaa acttgagcag gagcttcgtc aacactaccg ctgcggcgac tatcttcccg
     4141 ccgagcagca actggcagcg cgctttgagg tgaaccgcca caccctgcgc cgcgccatcg
     4201 accaactggt ggaaaaaggt tgggtacagc gccgtcaggg cgtcggcgtg ctggtgctga
     4261 tgcgcccgtt cgattacccg ctcaacgccc aggcgcgttt tagccagaac ctgctggacc
     4321 aaggcagcca tcccaccagc gaaaaattgc tatcggtgct gcgccccgcc tccggtcacg
     4381 tcgctgacgc gctggggata accgaggggg agaacgtcat ccacctgcgc accctgcgcc
     4441 gggtcaacgg cgtcgcgctc tgtttaatcg accactactt tgcggacctc accctctggc
     4501 cgacgctgca acatttcgac agcggttcgc tgcacgattt tctgcgcgag caaacgggga
     4561 ttacgctacg ccgcagtcag acgcggatca gcgcccgccg cgcccaggcc aaagagtgcc
     4621 agcgtcttga aatcccgaat ctatcgccgc tgctgtgcgt gcgcaccctt aaccaccgtg
     4681 acggtgaaag cagcccggcg gagtactccg tcagcctgac gcgcgccgac atgattgaat
     4741 tcactatgga gcactgaatg cacgcagata ccgcgacccg ccagcactgg atgtccgtac
     4801 tggcgcacag ccaaccgact gagctggcag cccgcctgaa cgcgctgaac atcaccgccg
     4861 actatgaagt aatccgcgcc gctgaaaccg gcctggtgca gattcaggcg cggatgggtg
     4921 gtaccggcga acgctttttt gctggcgacg ccacgctcac ccgcgccgcc gtacgcctga
     4981 ctgacggcac gctcggctac agctgggtgc tggggcgcga caaacagcac gccgaacgct
     5041 gcgcgctgat tgacgcgctg atgcagcaaa cccgccattt tcaaaactta tcagaaactc
     5101 ttattgcccc gctgaacgct gaccgtatgg cacgcattgc cgcacgccag gccgaagtaa
     5161 acgccagccg ggtcgacttc tttacgatgg ttcgcggaga taacgcatga ccctggaaac
     5221 cgcttttatg cttcccgtgc aggatgccca gcacagtttt cgtcgcctgt taaaagccat
     5281 gagcgagccg ggcgtgattg tcgccctgca tcagctcaaa cgcggctggc agccgctgaa
     5341 tatcgccacc accagcgtgc tgctgacgct ggccgataac gacacgccgg tgtggctttc
     5401 tgcgccatta agtaacgata tcgtcagcca gagcctgcgt tttcatacca acgcgccgct
     5461 ggtcaatcag ccggaactgg cgaccttcgc ggtaacggat gaggcgattt ccagcgaaca
     5521 gcttaacgcc ctttccagcg gcaccgctgt tgcgccagaa gcgggcgcga cgctgatttt
     5581 acaggtcgtc agcctgagcg gcgggcgcat gctgcgcctt actggcgcgg gtattgccga
     5641 agaacgaatg atcgccccgc agctgccgga gtgcattctg cacgaactta ccgagcgccc
     5701 gcacccattc ccgctcggca tcgacctgat cctgacctgc ggcgagcgcc tgctggctat
     5761 cccgcgaacc actcatgtgg aggtatgctg atgtacgttg ccgtgaaagg gggcgagaag
     5821 gcgatcgacg ccgcccacgc cctgcaagag agccgacgcc ggggcaatac cgatttgcct
     5881 gaactgagcg tcgcccagat tgaacagcag cttaatcttg cagtagatcg cgtgatgacc
     5941 gaaggcggca ttgccgaccg cgaactggcg gcgctggcgc tgaaacaggc cagcggcgat
     6001 aacgtcgaag cgattttcct gctacgcgcc taccgcacca cgctggcgaa gctcgcagta
     6061 agcgagccgc tcgacaccac ggagatgcgt ctcgaacggc gtatttcagc ggtttataaa
     6121 gatattcccg gcggccagct gcttggccca acctacgact acacccatcg cctgctcgat
     6181 ttcaccctgc tggcaaacgg cgaagcgccg acgctgacca cagccgacag cgaacaacag
     6241 ccgtcgccgc acgttttcag cctgctggcg cgtcaggggc tggcgaagtt tgaagaagat
     6301 agcggcgcac agccggacga catcacccgc acgccgccgg tttacccctg ctcacgctcc
     6361 tcccgcctgc aacaattaat gcgcggcgac gaaggctatt tgctggcgct ggcctactcc
     6421 acccagcgtg gttacggacg caatcatccg ttcgcaggcg aaatccgcag cggctatatc
     6481 gacgtgtcga ttgtgcagga agagctggga tttgcggtga acgtcggcga actgctgatg
     6541 accgaatgtg aaatggtcaa cggttttatc gacccgccgg atgagccgcc gcacttcacg
     6601 cgcggctacg ggctggtgtt cggcatgagc gagcgcaaag cgatggcgat ggcgctggtt
     6661 gaccgcgctc tacaagcccc ggagtacggc gagcacgcga caggcccggc gcaggatgaa
     6721 gagttcgtgc tggcgcatgc cgacaacgtc gaagccgcag gctttgtctc gcaccttaaa
     6781 ctcccgcact acgtcgattt ccaggccgaa ctggagctac tcaaacgtct gcaacaggag
     6841 cagaaccatg gctaatctga gcggctacaa ctttgcctac ctcgacgagc agaccaaacg
     6901 catgatccgc cgcgccatct taaaagcggt ggcgatcccc ggttatcagg tgccgtttgg
     6961 cgggcgcgag atgccgatgc cgtacggctg gggaaccggc ggcatacagc ttaccgccag
     7021 cgtgattggc gaaagcgatg tgctgaaggt gattgaccag ggcgcggacg acaccaccaa
     7081 cgcggtgtcg atccgtaact tctttaagcg cgtgaccggg gtaaacacca ctgaacgtac
     7141 ggacgatgcg acgcttatcc agacgcgcca ccgcatcccc gaaacgccgc tgaccgaaga
     7201 tcagatcatt attttccagg tgccgatccc cgaaccgctg cgctttatcg agccgcgcga
     7261 aacggaaacc cgcaccatgc acgcgctgga ggagtacggc gtgatgcagg tgaagctgta
     7321 tgaagatatc gcccgcttcg gtcatatcgc caccacctac gcctatccgg tgaaggtaaa
     7381 cgggcgctac gtaatggacc cgtcgccgat cccgaaattt gataacccga aaatgaacat
     7441 gatgcccgcc ctgcaactgt tcggcgcggg gcgcgagaag cgcatctatg cggtgccgcc
     7501 gtttacccgc gtggaaagcc tcgatttcga cgatcacccg ttcaccgttc agcagtggaa
     7561 tgagccatgc gccatctgcg gatcgaccca cagctatctt gatgaagtgg tgctggatga
     7621 cgccggaaac cgcatgtttg tctgctccga taccgattat tgccgccaac agagcgaggc
     7681 gaaaagccaa tgaatcaacc gttactttcg gtcaataacc tgacccacct ttacgcgccg
     7741 ggcaaaggct ttagcgatgt ctcttttgat ttatggccgg gggaagtgct gggcatcgtc
     7801 ggggaatccg gctccgggaa gaccacgctg ctgaagtcga tctccgcgcg cctgacgccg
     7861 cagcaagggg aaattcgcta tgaaaaccgt tccctctatg gcatgagcga ggccgaccgc
     7921 cgtcgcctgc tgcgcaccga atggggcgtg gtgcatcagc atccactcga cggcctgcgc
     7981 cgccaggtgt cggcaggcgg caatatcggc gagcggctga tggcgaccgg ggcgcgtcat
     8041 tacggcgata ttcgtgccac cgcgcagaag tggctggaag aggtggaaat tcccgccaac
     8101 cggatcgacg acctgccgac caccttttcc ggcggtatgc agcagcgttt gcagattgcc
     8161 cgcaacctgg tgacgcatcc gaagctggta tttatggacg agccaaccgg cgggctggat
     8221 gtatctgtgc aggcgcgcct gctcgacctg ctgcgcggcc tggtggtgga gctgaacctg
     8281 gcggtggtga ttgtcaccca cgatttaggc gtcgcccgcc tgctggcgga ccgtttgctg
     8341 gtgatgaagc aggggcaagt ggtggagagt gggttaaccg accgcgtgct cgacgacccg
     8401 caccatccgt atacacaact gctggtgtca tcggttttgc agaattgagc cggtgccgga
     8461 tgcggcgtaa acgccttatc cggcctacaa atgcgctccc cgtaggtcgg ataagacgcg
     8521 tcagcgtcgc atccgacacc cgaaccacga ggcgaaaaat gattaacgta caaaacgtca
     8581 gtaaaacctt catcctgcat cagcaaaacg gcgtgcgcct gcccgtctta caacgtgcct
     8641 cgctcaccgt caacgcaggc gaatgcgtgg tgcttcacgg ccactccggc agcggaaaat
     8701 caaccctgct gcgctcgctg tacgccaact atctgcccga cgaagggcaa atccagatca
     8761 aacacggtga ggaatgggta gacctggtca ccgcgccagc ccgcaaagtg gtggagatcc
     8821 gcaaaaccac catcggctgg gtgagccagt ttctgcgcgt catcccgcgt atttcggcac
     8881 tggaagtggt gatgcagcct ctgctcgata ccggcgtacc gcgtgaagcc tgcgccgcta
     8941 aagccgcccg attactgacg cgcctgaacg tgcctgaacg cctgtggcac ctggcaccat
     9001 cgaccttctc cggcggcgaa cagcagcgcg tcaacatcgc ccgtggcttt atcgtcgact
     9061 atccgattct gctgctggat gaacccaccg cctcgctgga cgctaaaaac agcgccgcgg
     9121 tggtggaatt aattcgcgaa gccaaagccc gtggcgcggc catcgtcggt atcttccatg
     9181 acgaagccgt acgcaatgac gtcgccgacc gcctgcaccc aatgggagtc tcttcatgat
     9241 tatcaataac gtcaaactgg tgctggaaaa cgaagtggtg cacggttcac tggaagtaca
     9301 ggatggcaaa attcgcgcct ttgccgaaag ccagagccgc cagccggaag cgatggacgg
     9361 cgaaggtggc tggctgctgc cggggctgat tgagctgcat accgataatc tggataaatt
     9421 cttcaccccg cgtccaaaag tggactggcc cgcccactcg gcaatgagta gccacgacgc
     9481 gctgatggtg gcgagcggca tcaccaccgt gctggacgcg gtggcgattg gcgacgtgcg
     9541 cgacggcggc gatcggctgg agaatctgga gaagatgatc aacgccatcg aagagacgca
     9601 gaaacgcggc gtcaaccgcg ccgagcaccg tctgcatctg cgctgcgaac tgccgcatca
     9661 caccacgctg ccgctgtttg aaaaactggt gcagcgcgaa ccggtgacgc tggtgtcgct
     9721 gatggatcac tcgccgggcc agcgccagtt cgctaaccgc gagaagtatc gcgaatatta
     9781 tcagggcaaa tactcgctca ccgaagcgca gatgcagcag tatgaagaag agcagctggc
     9841 gctcgccgca cgctggtcgc agccaaatcg cgaaaccatc gccgcaatgt gccgcgcgcg
     9901 acacattgcg ctcgccagcc acgatgacgc cacgcacgcc cacgttgctg aatctcacca
     9961 acttggcagc gtaatcgccg aatttcccac cacgttcgaa gcggcagaag cctcgcgcaa
    10021 gcatggcatg aacgtgctga tgggcgcacc gaatatcgtg cgcggcggtt cgcactccgg
    10081 caacgtggcg gccagtgaac tggcgcagct tggcctgctg gatatcctct cttccgacta
    10141 ctaccccgcc agcctgctcg atgcggcatt tcgcgtcgcc gatgacgaga gcaaccgctt
    10201 tacgctgccg caggcggtga agctggtgac taaaaatccg gcgcaggcgc ttaatcttca
    10261 ggatcgcggg gtgattggcg agggcaaacg cgccgacctg gtgctggcgc atcgcaaggg
    10321 caatcacatt catatcgacc acgtctggcg tcagggtaaa agggtgttct gatgacggga
    10381 aaactgattt ggttaatggg ggcctctggc tccggaaaag acagtctgtt gacggaactc
    10441 cgccagcggg aacaaactca gctactggta gcgcatcgct acatcacgcg cgccgccagc
    10501 gccggaagtg aaaaccatat cgccctgagc gagcaggagt tttttacccg cgcggggcag
    10561 aaccttctgg ccttaagctg gcacgctaac ggtctgtatt atggcgtcgg cgtcgagatt
    10621 gacctctggc tgcacgctgg atttgacgtg gtggtcaacg gctcacgcgc ccatctgccg
    10681 caggcgcggg cgcgctatca atcggcgctg ctgcccgttt gtttacaggt ttcgccagag
    10741 atcctgcgcc agcgcctgga aaaccgtggt cgtgaaaatg ccagtgaaat taacgcccgg
    10801 ctggcgcgcg ctgcccgcta tactcctcag gattgtctta cgctcaataa tgacggcagc
    10861 ctgcgccagt cggtcgacaa gttgctgacg ctgattcatc agaaggagaa acaccatgcc
    10921 tgcttgtgag cttcgcccgg ctacgcagta cgacaccgac gcagtttacg cgctgatttg
    10981 tgagctaaaa caggcggagt ttgaccacca ggcgtttcgc gtggggttta acgccaatct
    11041 gcgcgatccg aacatgcgct accaactggc gctgctggat ggcgaagttg tcggcatgat
    11101 cggcctgcat ttgcagtttc atctgcatca tgtcaactgg atcggcgaaa ttcaggaact
    11161 ggtggtgatg ccgcaggcgc gcggtctgaa tgtcggcagc aaattactgg catgggcaga
    11221 agaagaagcc cgccaggccg gggccgaaat gactgagctt tccaccaacg tgaagcgcca
    11281 cgacgcgcac cgattttacc tgcgcgaagg ctacgagcag agccacttcc gcttcaccaa
    11341 ggtgctgtaa catgagcctg accctcacac ttactggcac tggcggcgca cagggcgttc
    11401 ccgcatgggg ctgtgagtgc gtggcctgcg tcagagcgcg gcgctcaccg cagtatcgcc
    11461 gccagccatg cagcggcgtc gtgaagttta acgacgcgat caccctgatc gacgccgggc
    11521 ggcacgatct cgccgatcgc tggtcgcccg gatcgttcca gcagtttttg ctgacgcact
    11581 accatatgga tcacgtgcag gggctgtttc cgctgcgctg gggcgtgggc gatcagatcc
    11641 cggtctacgg cccgccggat gaacagggct gcgacgatct attcaaacat tcgggcctgc
    11701 ttgatttcag ccacacggtg gagccatttg tgatgtttga tttgcaggga ttacaggtca
    11761 cgccgctacc gctcaaccac tcaaaactca ctttcggcta tttactggaa acggcgcaca
    11821 gccgggtggc gtggctatct gacaccgcag gcctgccgga aaaaacgctg aaatttttac
    11881 gcaataatca gccgcaggta atggtgattg attgcagcca cccgccgcgc gaggatgcac
    11941 cgcgtaatca ctgtgattta aataccgtgc ttgcgctgaa tcaggttatc cgctcgccgc
    12001 gggtgattct gacccatatc agccatcagt ttgatgcgtg gctgatggaa aacgcgctac
    12061 catcggggtt tgaggcgggg tttgatggga tggagattga ggtggcgtga gggaaaatac
    12121 ccgcgccggc ccctatatca acgcccatac tcatcttcca ttctccgctc ttcatcttcc
    12181 agttgccgac gctcctgatc aagctggcgc tggcgatcgt ccagctgcct gcggcgatct
    12241 tcaaactggc ggcggcggtc atcatattgt ctgcgccgat cgtcgctcac ttcacgctgc
    12301 cagccgtcgt cgcgcgaatc ttcatagtct cgcccacggt cagggttata agcgtcgtta
    12361 atcgcctgct gaatattgcc aatggtgtcg tcgataatat cggcctgggc cggaacggtg
    12421 gacagcgtga gcagggtgaa taaaagaaat agcggaaagc gtttcattag ccaacctcaa
    12481 aaagaaactc tatccacatt agtcattact catccatgca agtagtggac gaatctcaat
    12541 ttctccgctg ctctattgcc gtaatcgcct ccacgcgttg ttgatgacga ccgccttcgt
    12601 actgtgcgcc cagccacgca tcgacaatca tttttgccag ttcgaggcca accactcgtg
    12661 aaccaaaagc cagcacgttg gtgtcgttat gctgccgcga aagttgcgcg gaataaggtt
    12721 cgctacaaac gaccgcgcga attccggcaa acttgttcgc cgctatcgaa ataccgacgc
    12781 cagtgccaca aatcaaaatc ccgccatcag cctctccgcc agcaacggcc agtgcgacgt
    12841 gactggcgta atgcggataa tcagtacgct ctgacgacca ggttccttta tcaatcactt
    12901 caacgccacg ctcaactaaa tgtgccacta tttcatgttt taaaatgaaa ccgacatgat
    12961 cacagccaaa tgcaatcttt ttcattcatc ttccccgccc gcgcaaaact tcacaatcaa
    13021 aaaacatttg tgatgtaaat gaattaaaaa cacccaaaat cgatgaaatc cgccttcccg
    13081 atctgtttta cagagttaca tctggcagcg caatagcgtt aaagacactc actatccagc
    13141 aacatctatc atctaataaa ccaggaaaac aaataacatc atgtttttaa agtgattaaa
    13201 tgaaatgaaa ttttaagcca ctctccatta ttcacaacaa aacaaacttt ataaatttta
    13261 tttttttgtg aagttgccag catcttttct gttcttgctg tggtgatata gtggcgtctt
    13321 caattcaagg acaagagaac gtgatgagcc agtcagagtt tgattcagcg cttccgaacg
    13381 gtatcgggtt agcgccttac ctgcgaatga agcaggaagg aatgacagaa aacgaaagcc
    13441 gcatcgtgga gtggttactc aaacccggta acctgagttg tgcacccgca attaaagatg
    13501 tcgcagaagc tctggcggta tctgaagcga tgatcgttaa ggtatcaaag ctgctggggt
    13561 ttagcggctt tcgtaactta cgcagtgcgc tggaagatta tttttctcag tcagaacaag
    13621 tattgccttc cgagttggct tttgatgaag cgccgcagga tgtggtgaat aaggtattta
    13681 acatcacttt acgcaccatt atggaaggtc agtcgatcgt caacgttgat gagatccacc
    13741 gtgccgcccg ctttttctat caggccagac agcgggattt gtacggtgcc ggaggatcaa
    13801 atgcaatctg tgctgatgta cagcacaagt ttttgcgcat tggcgtacgc tgccaggcct
    13861 atccggacgc tcacatcatg atgatgtctg cttcgttgtt acaggaagga gatgtggtgc
    13921 tggtggtgac ccattccggg cgaaccagtg atgtaaaagc ggctgtagaa ctggcaaaaa
    13981 agaacggggc aaagattatt tgtataacgc atagctacca ttcaccgata gcgaagctgg
    14041 ccgattatat tatttgctca ccggccccgg aaacaccgtt attaggtcgt aatgcctcgg
    14101 caagaatatt acaactaact ttgctggacg cgttttttgt ctctgtcgcc cagctcaaca
    14161 ttgaacaagc taatattaat atgcaaaaaa ccggcgcaat tgttgatttc ttctcaccag
    14221 gcgcgctgaa ataagaaaaa ggtaattccg ccatcgcgga gttacctatc cccttaccgt
    14281 gagaacatga ttatgaataa atatctgaaa tatttcagcg gcacactcgt gggcttaatg
    14341 ttgtcaacca gcgcttttgc tgccgccgaa tatgccgtcg tattgaaaac actctccaat
    14401 ccattttggg tagatatgaa aaaaggcatt gaagatgaag caaaaacgct gggcgtcagc
    14461 gttgatattt ttgcctctcc ttcagaaggc gattttcaat ctcaattgca gttatttgaa
    14521 gatctcagta ataaaaatta caaaggtatc gccttcgcac cattatcctc agtgaacctg
    14581 gtcatgcctg tcgcccgcgc atggaaaaaa ggcatttatc tggtcaatct cgatgaaaaa
    14641 atcgacatgg ataatctgaa aaaagcaggc ggtaatgtgg aaggttttgt caccaccgat
    14701 aatgttgccg tcggggcgaa aggcgcgtca ttcattattg acaagctggg tgccgaaggg
    14761 ggtgaagtcg caatcattga gggtaaagcc ggtaacgcct ccggtgaagc gcgtcgtaat
    14821 ggtgccaccg aagccttcaa aaaagcaagc cagatcaagc ttgtcgccag ccagcctgcc
    14881 gactgggacc gtattaaagc actggatgtc gccactaacg tgttgcaacg taatccgaat
    14941 attaaagcga tctattgcgc gaatgacacg atggcaatgg gtgttgctca ggctgtcgca
    15001 aacgccggaa aaacgggaaa agtgctggtc gtcggtacag atggcattcc ggaagcccgc
    15061 aaaatggtgg aagccggaca aatgaccgcg acggttgccc agaacccggc ggatatcggc
    15121 gcaacgggtc tgaagctgat ggttgacgct gaaaaatccg gcaaggttat cccgctggat
    15181 aaagcaccgg aatttaaact ggtcgattca atcctggtca ctcaataaaa ctaaatgatg
    15241 ccggatggtg atgctaatga agtgtcttat ccggccaaca aaataacctg aacgtagacc
    15301 tgataagtgt agcccatcag gcaataatac gatatttcgt gcagagaggt tatttatggc
    15361 cacgccatat atatcgatgg cggggatcgg caagtccttt ggtccggttc acgcattaaa
    15421 gtcggttaat ttaacggttt atcctggtga aatacatgca ttactaggag aaaatggcgc
    15481 gggtaaatcc acgctaatga aagttttatc cggaatacat gagccgacca aaggcaccat
    15541 taccattaat aacattaact ataacaaact ggatcataaa ttagcggcac aactcggcat
    15601 cgggattatt tatcaagaac tcagcgttat tgatgaatta accgtactgg aaaatttata
    15661 tattggtcgt catctgacga aaaaaatctg tggcgtcaat attatcgact ggcgagaaat
    15721 gcgtgtccgc gccgccatga tgttattacg cgtgggcttg aaagttgatt tagatgagaa
    15781 agtggcgaat ttatctatca gccacaagca gatgctggaa attgccaaaa cgctgatgct
    15841 cgacgccaaa gtcatcatca tggatgaacc cacctcctca ctcactaata aagaggtgga
    15901 ctatttgttt ctgatcatga atcagttgcg taaggagggt acggccatcg tctatatctc
    15961 gcataaattg gcggaaattc gccgtatttg cgaccgctat acggtgatga aagacggcag
    16021 cagcgtttgc agcggcatgg tgagcgatgt gtcaaatgac gatatcgtcc gtctgatggt
    16081 aggtcgcgaa ctgcaaaacc gttttaacgc gatgaaagag aatgtcagca accttgcgca
    16141 cgatacggtt tttgaggtgc gcaacgtcac cagccgtgac agaaaaaagg tccgggatat
    16201 ctcatttagc gtctgccggg gagaaatatt aggctttgcc ggactggtcg gttccggacg
    16261 tactgaactg atgaactgcc tgtttggcgt tgataaacgc gctggcggag aaatccgtct
    16321 taatggcaaa gatatctctc cacgttctcc cctggatgcc gtgaaaaaag ggatggctta
    16381 catcactgaa agccgccggg ataacggctt tttccccaac ttttctatcg ctcagaacat
    16441 agctatcagc cgcagtctga aagacggcgg ctataaaggc gcgatggggt tgtttcatga
    16501 agttgacgag caacgtaccg ctgaagccca gcgcgaactg ctggcgctga aatgtcattc
    16561 ggtaaaccag aacatcactg aactctccgg cggtaaccag cagaaagtcc tgatctccaa
    16621 atggctgtgc tgtcacccgg aagtgatcat tttcgatgaa cctacccgtg gcatcgacgt
    16681 tggcgcgaaa gccgaaattt acaaagtgat gcgccaactg gcggacgacg gaaaagtcat
    16741 cctgatggtg tcatctgaac tacctgaaat tatcaccgtc tgcgaccgca tcgccgtgtt
    16801 ctgcgaagga cgactgacgc aaatcctgac caatcgcgat gacatgagcg aagaggagat
    16861 tatggcatgg gctttaccac acgagtaaaa agcgaagcga gcgagaagaa accgttcaac
    16921 tttgcgctgt tctgggataa atacggtact ttttttatcc tggcgatcat cgtcgccatt
    16981 tttggttcgc tgtcgccaga atattttctg accaccaata atattaccca gatttttgtg
    17041 caaagctccg tgacggtact gatcggcatg ggggaattct tcgctatcct ggtcgccggt
    17101 atcgaccttt cggttggcgc gattctggcg ctttccggta tggtgaccgc taaactgatg
    17161 ttggcaggta ttgatccgtt tctcgcggcg ctaattggcg gtgtactggt tggcggcgca
    17221 ctgggggcga tcaacggctg cctggtcaac tggacgggac tacacccgtt cattatcacc
    17281 cttggcacca acgctatttt ccgtgggatc acgctggtga tctccgatgc caactcggta
    17341 tacggcttct catttgactt cgtgaacttc tttgccgcca gcgtaattgg gatacctgtc
    17401 cccgttatct tctcgctaat tgtcgcgctc atcctttggt ttctgacaac gcgtatgcgg
    17461 ctcgggcgca acatctacgc actgggcggc aacaaaaact cggcgttcta ttccgggatt
    17521 gacgtgaaat tccacatcct ggtggtgttt atcatctccg gtgtttgtgc aggtctggca
    17581 ggcgtcgtct caactgcacg actcggtgcc gcagaaccgc ttgccggtat gggttttgaa
    17641 acctatgcca ttgccagcgc catcattggc ggcaccagtt tcttcggcgg caaggggcgc
    17701 attttctctg tggtgattgg cgggttgatc atcggcacca tcaacaacgg tctgaatatt
    17761 ttgcaggtac aaacctatta ccaactggtg gtgatgggcg gattaattat cgcggctgtc
    17821 gcccttgacc gtcttatcag taagtaagga attgaacatg aaaatctccc cctcgttaat
    17881 gtgtatggat ctgctgaaat ttaaagaaca gatcgaattt atcgacaacc atgccgatta
    17941 ctttcatatc gatatcatgg acggccactt tgtgccgaat ctgacgctct caccgttctt
    18001 cgtaagccag gtaaaaaaac tggcgagtaa accgctcgac tgccatctga tggtaacgcg
    18061 tccgcaggat tacattgctc aactggcgcg tgcgggagct gatttcatca ctctgcatcc
    18121 ggaaaccatc aacggccaag cgttccgcct gattgatgaa atccgccgtc atggcatgaa
    18181 agtggggctg attcttaatc cggaaacgcc ggttgaggcc atgaaatact atatccataa
    18241 ggctgataaa attacggtca tgactgtcga tcccggtttt gccggacaac cgttcattcc
    18301 tgaaatgctg gataaacttg ccgaactgaa ggcatggcgt gaacgagaag gtctggagta
    18361 cgaaattgag gtggacggtt cctgcaacca ggcaacttac gaaaaactaa tggcggcagg
    18421 ggcggatgtc tttatcgtcg gcacttccgg cctatttaat catgcggaaa atatcgacga
    18481 agcatggaga attatgaccg cgcagattct ggctgcaaaa agcgaggtac agcctcatgc
    18541 aaaaacagca taacgtcgta gcgggcgtgg atatgggggc aacgcatatc cgcttttgtc
    18601 tgcggacagc agaaggtgaa acgctacact gcgaaaaaaa gcggaccgca gaagttattg
    18661 ctcccgacct ggtgtcgggt atcggcgaaa tgattgacga gcaactcagg cgctttaacg
    18721 ctcgctgtca tggtctggtg atgggatttc cggcgctggt cagtaaagat aaacgcacca
    18781 ttatttctac gcctaacctg ccgttaacag cggcagattt atatgatctc gccgataagc
    18841 tcgaaaatac gctgaattgt ccggttgagt tttcccgcga cgttaacctg caactctcct
    18901 gggacgtagt agaaaatcgc cttacgcaac aactggttct ggcagcctat ctcggtacgg
    18961 ggatggggtt cgcagtgtgg atgaacggtg cgccgtggac gggtgcgcac ggtgtggcag
    19021 gcgaactggg ccatatcccc ctgggagata tgacccaaca ctgcgcgtgt ggcaattctg
    19081 ggtgcctgga aaccaattgc tctggaatgg cgctaagacg ctggtacgaa caacagcccc
    19141 gaaattaccc attgagcgat cttttcgtcc atgcggaaaa cgcccctttc gtccagagtc
    19201 tgcttgaaaa cgcggcacga gccattgcca ccagcattaa tctgttcgat cccgatgcgg
    19261 tgattctggg tggtggcgtg atggatatgc ccgccttccc acgcgagact ctcattgcca
    19321 tgacccaaaa gtacctgcgc cgtccactgc cgtatcaggt tgtgcgcttt attgccgcct
    19381 catcttctga ctttaatggc gctcagggtg cagcaatatt ggcgcatcaa cgttttttgc
    19441 cacagtcctg tgctaaagtc ccatgagtaa agcgttggca cagtagcgca atgtcccgct
    19501 gtgccaac
//
LOCUS       DAZUCC010000085        19426 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZUCC010000085 DAZUCC010000000
VERSION     DAZUCC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.085"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHY09_002568"
     CDS             complement(72..818)
                     /gene="cutC"
                     /locus_tag="ACHY09_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS3885526.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHY09_002569"
     CDS             complement(832..1398)
                     /gene="yecM"
                     /locus_tag="ACHY09_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS3885527.1"
                     /translation="MANWHSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1614..3347
                     /gene="argS"
                     /locus_tag="ACHY09_002570"
     CDS             1614..3347
                     /gene="argS"
                     /locus_tag="ACHY09_002570"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS3885528.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHY09_002571"
     CDS             complement(3400..3792)
                     /gene="flhe"
                     /locus_tag="ACHY09_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFS3885529.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAIEPL
                     RFIWEVSGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(3792..5870)
                     /gene="flhA"
                     /locus_tag="ACHY09_002572"
     CDS             complement(3792..5870)
                     /gene="flhA"
                     /locus_tag="ACHY09_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFS3885530.1"
                     /translation="MSNLAAMLRLPANLKSTHWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5863..7011)
                     /gene="flhB"
                     /locus_tag="ACHY09_002573"
     CDS             complement(5863..7011)
                     /gene="flhB"
                     /locus_tag="ACHY09_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFS3885531.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKSILVGSVTG
                     FYLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7200..7844)
                     /gene="cheZ"
                     /locus_tag="ACHY09_002574"
     CDS             complement(7200..7844)
                     /gene="cheZ"
                     /locus_tag="ACHY09_002574"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFS3885532.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7855..8244)
                     /gene="cheY"
                     /locus_tag="ACHY09_002575"
     CDS             complement(7855..8244)
                     /gene="cheY"
                     /locus_tag="ACHY09_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFS3885533.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8259..9308)
                     /gene="cheB"
                     /locus_tag="ACHY09_002576"
     CDS             complement(8259..9308)
                     /gene="cheB"
                     /locus_tag="ACHY09_002576"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFS3885534.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9311..10171)
                     /gene="cheR"
                     /locus_tag="ACHY09_002577"
     CDS             complement(9311..10171)
                     /gene="cheR"
                     /locus_tag="ACHY09_002577"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFS3885535.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNLHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10190..11791)
                     /gene="tap"
                     /locus_tag="ACHY09_002578"
     CDS             complement(10190..11791)
                     /gene="tap"
                     /locus_tag="ACHY09_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFS3885536.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLESASAQSQRNYHISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(11837..13498)
                     /gene="tar"
                     /locus_tag="ACHY09_002579"
     CDS             complement(11837..13498)
                     /gene="tar"
                     /locus_tag="ACHY09_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFS3885537.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(13643..14146)
                     /gene="cheW"
                     /locus_tag="ACHY09_002580"
     CDS             complement(13643..14146)
                     /gene="cheW"
                     /locus_tag="ACHY09_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFS3885538.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14167..16131)
                     /gene="cheA"
                     /locus_tag="ACHY09_002581"
     CDS             complement(14167..16131)
                     /gene="cheA"
                     /locus_tag="ACHY09_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFS3885539.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRDQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16136..17062)
                     /gene="motB"
                     /locus_tag="ACHY09_002582"
     CDS             complement(16136..17062)
                     /gene="motB"
                     /locus_tag="ACHY09_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFS3885540.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17059..17946)
                     /gene="motA"
                     /locus_tag="ACHY09_002583"
     CDS             complement(17059..17946)
                     /gene="motA"
                     /locus_tag="ACHY09_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFS3885541.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18073..18651)
                     /gene="flhC"
                     /locus_tag="ACHY09_002584"
     CDS             complement(18073..18651)
                     /gene="flhC"
                     /locus_tag="ACHY09_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFS3885542.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(18654..19004)
                     /gene="flhD"
                     /locus_tag="ACHY09_002585"
     CDS             complement(18654..19004)
                     /gene="flhD"
                     /locus_tag="ACHY09_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFS3885543.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4595 a         5433 c         4783 g         4615 t
ORIGIN      
        1 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
       61 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
      121 gccccgtcta cgacataacg cgaatactcg tccgcgtgtg catctgatga catggacaat
      181 ccttgattac gataacgcat tggtgaggct tgccacgctc ccgcggagct atggacttcc
      241 agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc tccggccata
      301 atgattggag catcaccatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
      361 tctgattttt gccctgatgt cagtacccga gcaacgccca attccgcaag attattgagt
      421 gtatttaaag ggttagcgca catatcgaag gcgcgatgaa aagtcacggc cagcggaccg
      481 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac atcgagaaca
      541 cccgtcacca gtccaggaaa acctaattcg cggactgtgc gcacatcctc aagaatggcg
      601 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaataat aggatgcaca
      661 gggatcgtca cccgctggcg cacggatttc agcaccccca acgacggcgt taagcccccc
      721 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagcgcg
      781 cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta attacgccga
      841 ctgctcgctg gcaacgatct cttcaatgga ccacggatga aatttaatgg tcgttttgcc
      901 atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg gcgaactggt
      961 tttaactgag atccccggca ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     1021 attcagcgtt tctggatcgc ccggcagaac gatttcaata tgttcccaac cttcgtgcgg
     1081 gtaacgtttt tccccgggcc acggtagctc cacaatggaa aactgccagt gcgcaacctg
     1141 taccggttca tgcaatttaa acagacaaat cggcctgcca ttgatcatat tttctgacaa
     1201 aagctcgccg cactgttcaa acccgcgacg ccagcgctca gcggtggcgt tttgatggca
     1261 acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccaaac ggcgggaaag
     1321 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcgtcgat
     1381 agaatgccag ttcgccataa tcactcttcg tcttccacta aaagcgttaa tttaccctgt
     1441 tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacgaca
     1501 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     1561 tctttaactg gtgcgggtaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     1621 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     1681 attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat caagctaacg
     1741 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     1801 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgcgggt cctggcttta
     1861 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     1921 atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac tctgcgccaa
     1981 acgtggcgaa agagatgcac gttggtcacc tgcgctctac cattattggt gacgcggcag
     2041 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
     2101 gcactcagtt cggtatgttg attgcgtggc tggaaaagca gcagcaggaa aacgccggtg
     2161 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
     2221 atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat
     2281 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac cagatcacct
     2341 acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggtgaaagc ctctacaacc
     2401 caatgctgcc gggtatcgtg gcggacctga aagccaaagg tctggcagta gaaagtgaag
     2461 gcgcgaccgt cgtattcctt gatgagttca aaaacaagga aggcgaaccg atgggcgtga
     2521 tcattcagaa gaaagatggt ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
     2581 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
     2641 aacacctgat gcaggcgtgg gctatcgtcc gtaaagcagg ctatgtaccg gaatccgtac
     2701 cgctggaaca ccacatgttc ggcatgatgc tgggcaaaga cggcaaaccg ttcaaaaccc
     2761 gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga agccctggaa cgtgcacgcc
     2821 gtctggtggc agaaaagaac ccggatatgc cagccgacga gctggaaaaa ctggctaacg
     2881 cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
     2941 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
     3001 catacacgcg tgtattgtcc gtgttccgta aagcagaaat tgacgaagag caactggctg
     3061 cagctccggt tatcatccgt gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
     3121 ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg tgtgcttacc
     3181 tgtacgatct ggccggtctg ttctctggtt tctacgagca ctgcccgatc ctcagcgcag
     3241 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacggcg aagacgctga
     3301 aactgggtct ggatacgctg ggtattgaga cagtagagcg tatgtaatcg cgttatacgg
     3361 caaaagccga tgtcatggca gacatcggct tttttatcat taacgataat tcacaatcac
     3421 ttcattccgt tgtaccttta gcggcggaat taaccgccca ccgccagaca cttcccagat
     3481 aaatcgcaac ggttctatcg ccggtatacc ggaaaaggcc acggtggttc cactctgccc
     3541 ttctaattcg acacaacgag attgtgaaca caagcgaacc cgcagtcctg caggtgtcgg
     3601 gccgataagc tgataacgcc acgccaccag cgtcatcaat cctgcagcag gttgtcgagt
     3661 agaaagaggc gctgacgaca tcgactcacc acggtgattc agcgtaacgc ccacactact
     3721 cgcctgccac atcccctccc cggcggcttg caccagcagc ggaaacaata atattgctaa
     3781 taaagttctc atcatttacc gccaattgtc gccgtcatgc ggatatgtcg gttatcagat
     3841 agttccagat tcgacagcac cactaactgc ggcaggctgc ggcgcaggaa gcgagacaat
     3901 aatggtcgca gcgcgtggtt caccaacaat accggcggcg cacccaacat ctcctgacgg
     3961 gatagcgctt cctgagtttg cgccagtaaa cgatccgcca gccctggctc cagtcccccc
     4021 ccgccctgca acgcctgtag caacaaacgt tccagcggtg tatcgaggcc aataacatgg
     4081 acttcatctt tgccaggaaa ccactgctgg gtaatcgccc ggcccaacgc tacgcgcacg
     4141 acggcggtta attcatgcgg atcgctttgg atgggcgcat gttccgccag cgtttcgaga
     4201 atggtgcgca tatcgcgaat cggcactttt tcatcgagca gattttgcag cactttatgc
     4261 agtgtggtaa gcgtgacgac accaggaacg agatcttccg tcagctttgg catctcctgg
     4321 gcgacgcgat ccagcagctg ttgtgcctcc tgacgaccaa acagctctgc ggcatgctgg
     4381 ctaatgaggt ggttaagatg ggttgccacc accgtgctgg cctcaaccac ggtgtacccc
     4441 tgaatctgcg cctgttcttt aagcgcactt tcaatccaga tagcattcag gccaaatgcc
     4501 ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg taccagggtt aatcgccaac
     4561 caacgccccg gataagcatc accactgcct atctccacgc ctttcatcaa aatgcgatag
     4621 cgggcaggtt gcagatccat attgtcgcga atgtgcacca ccggcggcag aaaccccatc
     4681 tcctgggcaa atttcttgcg aatactgcgt atacggccca gcaactcacc atcctgctgg
     4741 aaatcgacca tcgggatcag gcgataaccc acttccattc ccagagaatc ttccagttgc
     4801 acatcgttcc acgtcgcttc gacaacggta ttattctctg ccatttttac cggttttggt
     4861 tcggcaggcg ctttttgttc acgtccgcgg atccaccagg ccagtcccag caatccggca
     4921 gtgaacagca aaaataccag gttcggcatt ccaggcacca ggccgagtaa accgagcacg
     4981 gcggcgctta acaacataac gcttgggtta ctgaaaagct gattcaccat ctgctcgcca
     5041 acatcctgat cggtgctgac acgcgtaacg atgacccccg cggcggtaga aatcaccaac
     5101 gccggaattt gtgccaccag accgtcgcca atagtcaata gcgtataact ttccgccgcg
     5161 tgtcccatgc tcataccatg ttgcagcacg ccgaccagca acccaccgac aacgtttatg
     5221 accatgatga ggatcccggc gatggcatcg ccgcgaacaa acttacttgc cccgtccatc
     5281 gagccgtaaa aatcggcttc ctgagtcact tcggagcggc gttttttcgc ttcatcttca
     5341 ccaatcaatc cggcgttaag gtcggcgtca atcgccatct gcttccccgg cataccatcg
     5401 agaacaaagc gcgcgcctac ttccgcaata cgcccggcac ctttggtaat gaccataaag
     5461 ttgatgatca cgagaatgac gaacaccacg ataccgatag cgaaattgcc accaacgagg
     5521 aagtgaccga acgcttcgac cacctttcca gccgccgccg cgccggtatg cccttccatc
     5581 aaaatgatac gggttgaagc cacattaagt gccagacgca acaacgtggt aaacaacaga
     5641 atcgtcggaa acgcagcaaa ctcaagcgtg cgttgggtaa acatcgccac cagcaacacc
     5701 atgatcgaca aggcaatatt gaaggtaaac agcaggtcga gtatgaatgc gggcagtggc
     5761 agtaccatca tcgacaagat caacaggatc aaaatcggtc cggcaaggat ctgccagtgt
     5821 gtcgatttca ggtttgcggg caggcgcagc atcgcggcca gattactcat gggtcggttt
     5881 ctcgttaata aaatccaggg cttccggcac cggaagatga gtaggttgta cagggcgctg
     5941 tccaccagcc agacgccagc gtttcagttg ccagacccag gccagcactt ccgccaccgc
     6001 ggcgtacagt tgacccggga tttgttgacc gatctccgca tgtcgataca gcgcacgcgc
     6061 cagcggcggc gcttcaagcg tcgggacgtt attttcagcg ccaatttcac gaatgcgcag
     6121 cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg ctcattttgt tttcgtcata
     6181 ctgcaaagcc actgagtagt gagttgggtt attgacgata acgtccgctt tcggcacatc
     6241 ggccatcatc cgccgccgcg ccgcagctcg ctgcatctga cggattcgcc ctttgacatg
     6301 agggtcgcct tcgctttgtt tgaactcatc acgaatatcc tgccgtgaca tacgcagctt
     6361 tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc accatcggaa tgacaccaag
     6421 caccaccagc aatgcgcaca gccctaccag atccatcgca ttacccatgg cggtaatggg
     6481 ggactccgcc attaagcgca tcatttgcgg ccagtggtgc cagagataaa accccgtcac
     6541 actgccgacc aggatggatt tcaaaatcgc tttaagcaac tccgcaccgg tttgtgccga
     6601 aaacatccgt ttaatgcccg gtagcgggtt gagtttggaa aacttcggct gcaaggattt
     6661 gccgctaaat accaggccac ccaacataac cggagaaata agcgccacca gcaccacacc
     6721 gctaatcagc ggcagcagcg ccagcatggc ttctctgatc agcagaataa tttgcccgag
     6781 gatcagattc gggtcattga taatactgtg atcaaaatgc agcccagcgg agagcatgcc
     6841 cgacaatcga cgggccagcg acacaccgcc aaaccagata acacacacgc ccactaacaa
     6901 aatcagcagt gacgttaatt cacgtgaacg cgggatttgc ccctcttccc gcgctttttc
     6961 tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat
     7021 cctggaaaga gtattaagcg taaatgatgc cagagcgcaa agcgttcaat ggtttgagta
     7081 aggggcaaaa caggcgggat ttagggcttt tactgccaca catcaagcat agtatgcgtt
     7141 tgtcggatgc ggcgtcatcg cctgatatga cgtggtcacg ccacatcagg caatacaaat
     7201 caaaatccaa gactatccaa caaatcgtcc acctgatcct gactggctac cacaccggct
     7261 ttgctggtat cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac
     7321 tcctgttccg ggatgttttc caacagcacc atcaacaact ggcgttcgat ctcctgaatg
     7381 acatccatca tccgcttaat aacctgcccg gtgagatcct gaaaatcctg cgccatcatg
     7441 atttccagca gttgcgcgtt agtaaagctg gtatgcgcgg ggacatctgc cagaaattgc
     7501 cgcgtatctg ttaccagttc tcgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca
     7561 tcccaacgtt gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac
     7621 gcctcaacac tgttcagcgc ccgctccgca gcctgggcgg tcatctgcac aacatagtac
     7681 aaacgatcgc gcgcatcggg gatggcttcc gccgcttcgg caatggcctg atccagcccc
     7741 agttcccgca aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg
     7801 ccagctgaat gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat
     7861 gcccagtttc tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggctt
     7921 caccacatag ccactggccc ccgcttgcgc cgcagcaata atgttctctt tcttcgcttc
     7981 tgcagtcacc attaacactg gcaatgctga catcgcgcca tccgcacgaa tcgttttcag
     8041 caattccagg ccatccatgt tgggcatgtt ccagtcggag ataacaaatc cataaccgcc
     8101 tgcctgcaac ttattgagag cgtcgacacc atcttccgct tcctcaacat tattgaatcc
     8161 tagctctttc agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa
     8221 aaatttaagt tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg
     8281 gcactaattt ttgccagcat ttgctggctt acctggctaa gatcgaccac ttcgcaaaca
     8341 ccacccatat tgatggcctc gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgt
     8401 gcaagggtcc atgcccccgc ctgacgcatc gccaacattc ccaccgcgcc gtcgttgccc
     8461 ataccggtca ggatcacccc aaccgcatta cgccccgcct gtttggcgac agaatggaac
     8521 aacacatcta ccgaaggccg atgacggtta accgccgggc catcgtgaat tttgatttgg
     8581 taatttgcgc cactacgcgc cagctccata tgccgatcgc ccggcgcaat ataagcatgc
     8641 cccggcaaga cacgttctcc gtcttcggct tctttaaccc cgatctggca aagcttatta
     8701 agtctgtcgg caaaagagcg ggtgaaaccg ggcggcatat gctgggtaat taacagtgcc
     8761 gggctggaaa gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa
     8821 gcaccaatcg caatcagttt ttcagagctc aacaacggcc ctgccttcag cgttgtcggt
     8881 gccgacaatg gcttatgtgc tgcaaggctc gcctttgctg ctgtacgcac cttttcagca
     8941 atcatttcgc tatacgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa
     9001 tctatcgccc ccagctccaa tgcgcgcagc gtgacttctg atcctttgcc ggtcagggaa
     9061 gaaaccatca caacgggcat tgggcgcaaa cgcattaatt tttcgagaaa atccagtccg
     9121 tccatccgcg gcatttcaac atccagcgtt agcacatccg gattgaattt cttaatcaaa
     9181 tcacgcgcaa ccagcggatc aggggcggtc gccaccattt ccatgtcgct atggctgttg
     9241 atgatttctg tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt
     9301 ttgctcatcg ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc
     9361 gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga
     9421 gcggaacaaa gcggcgcaaa atctcctgct gggtagtttg atcgaagtag atcatgacgt
     9481 tacgacagaa gatcgcatca aacggccccg gcacggtgta ctgtttcgcc agtaaattca
     9541 gcggggcaaa atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg
     9601 gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt
     9661 catggcgata aataccgctt ctggcttttt ccagcacttc ggtgtcgata tcgctggcaa
     9721 acactttcca gcgtccgggc acggtgccca atgtgtcagc cagcgtcatc gcaatgctgt
     9781 acggctcttc gccggtcgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac
     9841 gtgcgtgatc cgcgagcaga ggaaaatgat gcgcctcacg gaaaaatgcc gtcagattcg
     9901 tggtcagcga attgataaac gcctgccact caccgctgtg cagattagat tccagcaagt
     9961 tcaggtaatg accgaaatcc gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt
    10021 aaaccatgtc gcgtttatgg tcggccagga cgatcccggc tcgttgatag atcaattgac
    10081 ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag
    10141 acgtttgccc acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca
    10201 ctggcgcaat ttgtaactgc gccgactcat gtcgtgctac ttcatgctct tcaagggtaa
    10261 ataccgccac gcgcgacgaa agatggtcgg cctgattcgc cagttgttct gttgccaccg
    10321 ccgcttcttc taccagggag gcgttctgct gtgtcacctg atccatctgg ctgacagcct
    10381 gtgcaacctg ctcaatcccc cgccgttgtt cttccgaggc cgaagcaatt tctcccataa
    10441 tgtcgttcac gcgggtcacc gaactgacaa tatcagtcat ggtcgcggcg gcgttattca
    10501 ccagtttcga accctgctgg acacgattga ctgactcttc gatcagccct ttgatctctt
    10561 ttgctgcctg ggcgctacgg ctggcaagat tgcgcacttc acctgccact accgcaaaac
    10621 cacgcccctg ctctccggcg cgagccgctt ccactgccgc attcagggcc agaatattgg
    10681 tctggaaagc aatgccgtcg ataacgctga taatgtcgcc aattttttgc gagctggtag
    10741 cgatctcctg catggtgtga gtcatggtac tgacctgaac accgcccgcc tgcgccgttg
    10801 tcgcggcatt ttttgccagt tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg
    10861 tggcggttaa ttgctccata ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg
    10921 tgcgacttga gagatcgtta ttgcctgcga caatctccgc gataccaatg tgcatctcct
    10981 ggcttccctt acgcacatca cttaccgtcc cacgcaaagc ctgctgcatg gtcttcaggc
    11041 tggcaaaaat ggcggtgatc tcattgcgac catacaccgc aatcggacgt gccagattac
    11101 ccgcagcaat gctgtcaaaa tggctaccga taatggccag tggttgaaca atcatcttgc
    11161 gcgtccacca cagcgcactg ctgatgtaga tcgccgcaac aataatcatg ctgataaaca
    11221 ccagcgccga aatgtgatag ttacgctggc tttgcgcact ggcactttcc agcacatggt
    11281 tgatctccag ctgccaggcc tcaaagttaa cgtcaaacgc attctgtgat tcctgcaccg
    11341 gcgcagtgag gaaatccgaa agttgattac tttcaagcca ggtcgcctga tgttcgagat
    11401 cgttgtgcca gcgggcaaaa cttttctccg tctctttttg caacgccctg acgtgctcgt
    11461 tgcccgcagt catcgccata aaacttttaa acagcgtggt ggattgcgtc agactggcgc
    11521 gcgccgtcgt catcaacgtt ttaatgtcat ccgccggata gctaagcgcc gtcagagtgc
    11581 ccgctttgtt cagcgcggta ctggcctgta acattaccgc ccgagtttgc gctaatgccg
    11641 cacgttgctg attgctctgc tcgacctgat tcagtcgttg caaatcgtcg cgaaatgccc
    11701 aaaaagacat gccgttactg ccaatctgca agatcccgca gagaatcaaa attaaaaaca
    11761 gcgtggtcga aattcgaata cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg
    11821 cgttagtaaa tactcgtcaa aatgtttccc agtttggatc ttgttcagca attcgcagtc
    11881 gtggctgtgc cggtggttgc tcactggcag gacgggatgg tgtttgcggt ttattggtga
    11941 gtgggctggc tgccagacgg aacgcggaaa ccgcttgcgt taaacgactc gcctgttctt
    12001 ccagcgcagc tgcggcggca gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc
    12061 gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc acggctctgt tcatccgatg
    12121 ccgatgcaat ctcgcccata atgtcagtca cgcgggtgac agcattgacg atattgttca
    12181 ttgtttcccc ggcgctttcg accagcaccg aaccggtatc aacgcgcgag acggagtctt
    12241 caatgagggc tttgatctct tttgccgcct gggcgctgcg actggcaaga ttacgcactt
    12301 cgcccgccac cacggcaaaa ccacggccct gttcacccgc tcgcgcggct tcaacagcgg
    12361 cattcagcgc gagtatatta gtctggaagg caataccatc gataacgctg ataatgtcgg
    12421 caattttctt cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt
    12481 tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt
    12541 tatcggcgtt ttgcttcact gtcgcggtga gctgctccat gctggcggca gtttcttcca
    12601 gcgcggatgc ctgctgttcc gtacgggagg aaagatcggt gttgcccgcc gcaatttcac
    12661 gggtaccggc atagatggca tctgaacctt cacgcacatg agtgacggtg tcagtcaaag
    12721 agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg
    12781 tcagggtatt cgccaggtta ccaccggcga tttcgcgaat gtgagcaata atttttgcca
    12841 gcggtgtaag caacatacgg cgaatgccgt accacgccac cagcagaatc aataccacca
    12901 ccagcgcgat aaccgccagt tgccactggg caaatcggta atcatctgcg ttgtcagtga
    12961 cgatatcgcg atacagtttt tcactgctga gggcgtactg agcaaacgct tcgcccattg
    13021 cattttgcat tccctgggtt ggctgagcga aataagctcc agtattgcca taatcaagat
    13081 aatcaatcag ttcagttaac gctgtgtgat agtttttata tttttcatca atattacgac
    13141 tggtagcgac catttcaggt aacggtgcca tgcttttgaa ttttttataa tgagtcgctg
    13201 cctgggccaa tgttttcctg gcgctatcga gcaattcaac tttggcatta ctttgctgat
    13261 tggatgaatc catcatcatc cgcaccgctg aacgactcag gttaatgcgc gtttgcagca
    13321 ttaaatccca tgttgacgtc agctcgccct gctgttcccg taattgattg gaaaccacaa
    13381 agctcttctg gctatggtga agggaagaaa aaaacagact gccggaaata agctgtaaca
    13441 gtgcgaatac ccccagcacc attaccaaca gcgtgactac gcggatacgg ttaatcataa
    13501 ggcaccttcc tgaaaacaag ttgatctcgt tatcggcaag gaagggggaa actttattgc
    13561 tgatgccacc cgccgcgaaa ctgaaataaa aaacccgatg cgcagatcat cgggttcatt
    13621 gcaattgagg aaatcgggag aattacgcca cttctgacgc cgcgctatct aatagcgcca
    13681 tctcttcgct gttcagcagt ttttcgatgt tcaccagaat caacatacgg tcgcccagtg
    13741 cacccagtcc agtgagatat tctgttgaaa gcgtcacggc aaattccggt gccggacgaa
    13801 tttgctccgc cgtcaatgaa agcacgtctg agacgccgtc aaccacgatg ccgaccaccc
    13861 gctgtccgag attcaggacg ataactaccg tgttgtcgtt atagtccaca tccacctggc
    13921 tgaacttaat tcgtaagtcc acaatcggca caataacacc gcgcagattc gtgacgcctt
    13981 tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc gtagccacgg atctcctgca
    14041 ctttcagaat atcaataccg tactcttcat caccaagggt aaataccaga aattcctggc
    14101 ctgacggctc gctggccagc tttgttacat tcgtcatacc ggtcatattg ttaccttttt
    14161 actcattcag gcggcggtgt tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga
    14221 gacatcaaca atcagtgcca cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg
    14281 gactttgcga tagttacttt caaggttttt aaccacaacc tggtgttgac caattaattg
    14341 atccaccagc aaggcgtagc ggcgaccgcc actttgtaag atcaccacaa ttccctgggt
    14401 agcttcggtt ttcgcgcccg cgacgttgaa cactttccac agttcgacga tgggcagata
    14461 ttcaccccgc acttccagca cccgctcgcc gccagccagt ggatgcagat cggcttcacg
    14521 gggttgcagt gattccatga cagcattcag cggcagaatg aaaacttcat ccgcaacgcg
    14581 tacggacatg ccgtcgagga tggccagcgt cagcggcagt aaaatgcgga tcgtcgtgcc
    14641 agtaccctgc ttcgactgga tttcgacatg accgcccatc tcctggatat tacgtttaac
    14701 gacgtccatg ccgacgccgc gcccggagac gtcggtgacc tgctccgccg tggagaagcc
    14761 aggggcaaat atcagcatcg cgacttcgtc gtcgctcatg ttttcgctga cggtcagacc
    14821 ttgcgaggcc gcttttgcca gaattcgctc acggtttagc cccgcaccat cgtcggtcac
    14881 ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga attaaatttc cgacgctgtt
    14941 tttacctgcg gcgagccgtt tttctggcag ttcaataccg tgatcgaggc tattgcgtac
    15001 caggtgggtc agcgggtcga taatgcgttc tatcaggctc ttgtcgagtt cagtggaact
    15061 gcccaccagc gtcagttcta cctgcttgcc gagttttccc gcaagatcac gcaccagccg
    15121 gggatagcga ctaaagacat actccatcgg catcatgcga atcgacatca ctgattcctg
    15181 caaatcacgg gcgttacgtt gtaactgccc catgctggta atcaaatcac catgattaac
    15241 cgggtccagt tcactagaac gctgggcaag cattgactgg gtgatgacca gctcgccgac
    15301 gaggttaatt aattgatcaa ccttttctac cgctacacgg atgctggtgg attcactgct
    15361 gcgcgtcgtt ttttcccgct ccacgcggcc ggttggcgct tgttcggctg ccagtttaag
    15421 cactggtggg ggggatattt tgggcgagac ttctactgtt tcaaaggtaa tctgatcggc
    15481 ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg gcgatgtcgc ccggtaatat
    15541 tgccgagaga gaatccgccc ctttcaccac atcagttaac gttgtcagat gccccagctc
    15601 ttcttccagc aggtcgactt ccccggcctt caggcgcgaa aggataattc gtcgcggcga
    15661 ctgatcgcgg ctctgctcat cttgcggttc acttttggca accacactta atcgggtcac
    15721 tgcagatggc gtttcgcctt tcgcttctaa tgccagttga cgcaaggcct ggcagatata
    15781 atcgaagctg gcggcatccg gctcttgcga ctgtttataa gcgtcgagct gttcttgcat
    15841 gatgtccttc gtttccaaaa acagattgat aatgtcggtg ttgagttgca tctcacctcg
    15901 tctggcttcg tcgagcaggt tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc
    15961 aaaggttcct gcccctcctt tgatcgagtg ggcagcccga aagatggcat tcaattgttc
    16021 ggcatctggc gcttccggct gcaaaaccag cagatgctgc tccatgtcag ccaacagttc
    16081 gtccgcttca tcaaaaaatg tctgataaaa atcgcttata tccatgctca cgctgtcacc
    16141 tcggttcggc tgatggcatt gtgggaacac tgacctgtgg tgcaacctca ggtttttcca
    16201 gggcgcttac tggctcattc tggctttcgg cgttttcatg caaaatggcc tgttcggctt
    16261 gtttgttcag taccagcagg ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc
    16321 ttaagcgcat cgttgccgcc atgccgacga cacgtaacac tttgccgcca ttcaacccac
    16381 caaccatcag ttcgcggcgg gatgcattgg cacgatcggc agaaagctcc cagttgctgt
    16441 aacctttctc accgctggcg tagggaaaat catcggtatg acccgagagg ctaattcggt
    16501 tgggaatacc gttcagtacg ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga
    16561 cgtcggcact gccagttcta aacatcgggc gattctggct atcgatgatc tgaatacgta
    16621 gaccttcctg gaccagatcg attttgagat gtggacgtaa cgcccgcagt ttcggatcgg
    16681 actcaatcaa ctggtcgaga tcgccacgca atttccgcaa tcgactttgc tccatgcgtt
    16741 ttttcagctc ttcgatgttc ggctgcttat tcacttcccc ctggctttgg gtgtaatcat
    16801 cacccccacc gggaattggg ctttcactat tagaaatgcg atcgccgccc gtaaccgcag
    16861 tcgccagtgg agtccggaag tactccgcaa tctgaatcag ctcttttggg ctggagatgg
    16921 agattaacca catcaccaga aaaaaggcca tcatcgcggt cataaagtcg gcataagcaa
    16981 tcttccacga tccatgtgct gccccgtggc ttttggcttt gcgtcgtttg acgacaataa
    17041 tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac
    17101 cgcacgcaca tgctcttcca gttcaataaa cgacggacgt tcgctggaat agagcgtttt
    17161 gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc agattagata gcagtgtgac
    17221 tttgacgcac tgcatcattt tgctggtttc ggcgcttttc tgacgtaaaa cactcgctaa
    17281 tggggaaata aatccgtaag ccaataaaat accgaggaaa gtccccacca tcgcatgtgc
    17341 gataagcgcc cccagctcgg cggcaggacg atcggctgaa cctaacgcgt gaacgacccc
    17401 cattacagcc gcaacaatac caaacgccgg aagtgagtca ccgaccagcg ccagactgtt
    17461 cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct tcatccatca gagcttcgat
    17521 ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga taatcgacga taaattcaag
    17581 catgacgcta tccgcaagga tgcgtgggta gctggcgaag atctcgctct cacggggatt
    17641 ttcaatatca cgttccagcg aaaacatccc catctgccgc gatttcgcca tcaaccgata
    17701 aagcagagcc agcagatcca tatacattgc tttggtgtat ttggagcgac gaaacagcaa
    17761 cggcagcgcc ttcagcgtgc ctttaatcgc tttgccatta ttgccgacga taaacgaccc
    17821 aatccctgca ccggcaataa tcaccagttc agcgggttga tagagtgctc caaggcttcc
    17881 accggtcatc aaataaccgc cgaaaactgt accgagaaca accaggtaac ctaataagat
    17941 aagcacgaca tcatccttcc actgttgacc atgacaggat gttcagtcgt caggcgttaa
    18001 cacgcgatag gggcaaaaaa aagcagcggt acgtcgttac cgctgctgga atgtttcgcc
    18061 acaccgtatc agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc
    18121 ggcaggattc tgggaaagtt tacgtctttt tactgcccgg gatggcggct gacataagct
    18181 gcaggcaaag ctgccaacag gctggtgagc gtgggtaata aaattgccgc cgcagcagtt
    18241 gcagctggaa agttgcagta atccactttc aacaaaccgc accaatgtcc aggcacgggt
    18301 taatgccagc agcggtccct cttctgcttg cgggcactgt tcaaggtata aacggtaggc
    18361 tttgatcacc gcatcgacgc cattacacaa accggttttc agtaaaaact gccatgcgtt
    18421 acagaacatc gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg
    18481 cagcatgcct ttcggcggtg ggcttccgcg cagttcttta tagagtttta tcaggcgtcc
    18541 gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg tgatcaattc
    18601 cattgccagc tgaatatccc gcgcttcctg aacaatgctt ttttcactca tgatcaggcc
    18661 cttttcttgc gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg tgttgagagc
    18721 atgatgccgg tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt caactgagta
    18781 atcgtctggt gactgtcaaa acggaagtga caaaccagtt gattggtttc tgccagctta
    18841 accatttgcg gaagagtcag tgccgctaac gttgtcgcca tttcttcatt tatgccgaga
    18901 cgaaacatag cggacgcttt gtcctgaaca atcagacgct gtgcaagtag taaatatgac
    18961 aagttgatgt cataaatgtg tttcagcaac tcggaggtat gcattattcc cacccagaat
    19021 aaccaacttt atttttatgc ggtctcaccg caccccgtga tgtcgccggg aagccccggt
    19081 aaaaaataat tagcattaga atagttgcga taagctgcaa taggcagagc ccctttgtgg
    19141 gatttaataa gtccttacaa atagaagggg ttctttacac ttatctaaga tttttcctaa
    19201 atcgacgcaa ctttactcgt cactacacgc acatacaacg gaggggggct gcgattttca
    19261 ataatgtgtg atgcagatca cacaaaacgc tcaattactt aacataaatg tataagtcat
    19321 acttttgttt tgggtgtatt tcaatctgtt aaaaagtttt tcgcgacgct agcaagcaaa
    19381 aatgaaacag gaataataga aatgggatgt tgcgcacagt taaaat
//
LOCUS       DAZUCC010000086        19109 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZUCC010000086 DAZUCC010000000
VERSION     DAZUCC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.086"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..371)
                     /locus_tag="ACHY09_002586"
     CDS             complement(<1..371)
                     /locus_tag="ACHY09_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HFS3885544.1"
                     /translation="MAKMIPAFGPIDNNSYGEKTVYTLLKEGLSDDFTVIHSLPWLSA
                     ATRNLGMKLPPSGEIDFLVLHPVYGVLALEVKSGIYRVEGTVFVHIKTKKHVDIVKQT
                     RNNVHGLARWLGGATTLRLRIG"
     gene            complement(776..865)
                     /locus_tag="ACHY09_002587"
                     /pseudo
     CDS             complement(776..865)
                     /locus_tag="ACHY09_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1008..1583)
                     /locus_tag="ACHY09_002588"
     CDS             complement(1008..1583)
                     /locus_tag="ACHY09_002588"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFS3885545.1"
                     /translation="MLSADQIAQLSRTRSLLNHTSEWIPLSGQQITRLTELPLAYNLQ
                     RSAQLLQQLRVLFPDNPRVQEMVDNWQKSVRSRALPEEAMTGWNEGMTRLQQLAERLN
                     RLDEQRGKYMTVSELKTEVFGIMQAFNRHIPAEEQLRRYNEVRNQNGSEQQQKLTQDA
                     LTELLNRYWFLRHGDAGKKTINNKQDNQINI"
     gene            complement(1617..1766)
                     /locus_tag="ACHY09_002589"
                     /pseudo
     CDS             complement(1617..1766)
                     /locus_tag="ACHY09_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2091..2405)
                     /locus_tag="ACHY09_002590"
                     /pseudo
     CDS             complement(2091..2405)
                     /locus_tag="ACHY09_002590"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            complement(2460..2765)
                     /locus_tag="ACHY09_002591"
     CDS             complement(2460..2765)
                     /locus_tag="ACHY09_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885546.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            complement(2831..4576)
                     /locus_tag="ACHY09_002592"
     CDS             complement(2831..4576)
                     /locus_tag="ACHY09_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS3885547.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLMYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            complement(4811..6013)
                     /locus_tag="ACHY09_002593"
     CDS             complement(4811..6013)
                     /locus_tag="ACHY09_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS3885548.1"
                     /translation="MILVCVILWGIYVAVRALINLNERFIDAVSSPAGIIGLFFGLLT
                     VFAILLRFFIYRRLRKKTAAFEQAVSELVQRERGFNETVNAVIARGIRQEKEQLARRR
                     EEFHTARQKASRAMQRIVDSAWKFKAKTLLAGVTINNWQSKYDQLRKEREAYAAVSEK
                     IAFLNLEDNSDWESVRQQFLDKVALLEKAQEEKEYQAELKRQIREEKERQDELDRRQR
                     EAEEEERRLAEQQKLIEEALRAAEGAHREELEKQRLELEQKIQEAHAQYERAKSMAQL
                     TKQGHVYIISNIGSFGEDVFKIGMTRRLEPMDRVKELSGASVPFDFDVHAMISCDDAP
                     ALEKTLHDSLEKYRINRINLRKEFFRVKLEKIINEVERHHGQMEYVADPAALQYLQSL
                     EYAENEAA"
     gene            complement(6210..6512)
                     /locus_tag="ACHY09_002594"
     CDS             complement(6210..6512)
                     /locus_tag="ACHY09_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885549.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPAKRQVKSPVTTKL
                     VQKKKSAAVRKVSQPSVAGKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            complement(6859..7863)
                     /locus_tag="ACHY09_002595"
     CDS             complement(6859..7863)
                     /locus_tag="ACHY09_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS3885550.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKFGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            complement(8121..8552)
                     /gene="tssE"
                     /locus_tag="ACHY09_002596"
     CDS             complement(8121..8552)
                     /gene="tssE"
                     /locus_tag="ACHY09_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFS3885551.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            complement(8555..9091)
                     /gene="tssJ"
                     /locus_tag="ACHY09_002597"
     CDS             complement(8555..9091)
                     /gene="tssJ"
                     /locus_tag="ACHY09_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFS3885552.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWMQPGGSVTVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            complement(9072..10172)
                     /gene="tssG"
                     /locus_tag="ACHY09_002598"
     CDS             complement(9072..10172)
                     /gene="tssG"
                     /locus_tag="ACHY09_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFS3885553.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYLGSRLDVRLQLCVERSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(10127..11890)
                     /gene="tssF"
                     /locus_tag="ACHY09_002599"
     CDS             complement(10127..11890)
                     /gene="tssF"
                     /locus_tag="ACHY09_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS3885554.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENISLPAGISGFDIE
                     VVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVAGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEADRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPISVCNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            complement(12024..13622)
                     /gene="tssA"
                     /locus_tag="ACHY09_002600"
     CDS             complement(12024..13622)
                     /gene="tssA"
                     /locus_tag="ACHY09_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFS3885555.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKNIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLVELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNSGSS"
     gene            complement(13622..17011)
                     /locus_tag="ACHY09_002601"
     CDS             complement(13622..17011)
                     /locus_tag="ACHY09_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFS3885556.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTSPRYVTVQDI
                     RHTLRRQHGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPARFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(17004..18152)
                     /locus_tag="ACHY09_002602"
     CDS             complement(17004..18152)
                     /locus_tag="ACHY09_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFS3885557.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREIMDK
                     FCQLPAVLPVNVTLITDQPDSEIRSAFVSAWEVLFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCFGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(18156..18422)
                     /locus_tag="ACHY09_002603"
     CDS             complement(18156..18422)
                     /locus_tag="ACHY09_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFS3885558.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(18453..>19109)
                     /gene="tli1"
                     /locus_tag="ACHY09_002604"
     CDS             complement(18453..>19109)
                     /gene="tli1"
                     /locus_tag="ACHY09_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFS3885559.1"
                     /translation="FIAPLLALLTGCNSVSSQYADSDKTEWGYKNLPYDEWKFLFIYP
                     KALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWNDHLGATESYYNKGKALPLM
                     LRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLAPEGKVRV
                     WLGDRGNPVVPQLDAKITTVSGDKMNMCKGVTKHPDGYVYYGDTPDFIKGKIYPYGNW
                     "
BASE COUNT         4792 a         5306 c         4332 g         4679 t
ORIGIN      
        1 ccaatacgca accgtaacgt tgttgctccc cctaaccaac gggctaaacc atggacattg
       61 ttacgtgttt gcttcactat atctacatgt ttttttgttt ttatatgtac aaaaacagta
      121 ccttctacgc gatatattcc actctttacc tctaacgcca aaacaccata tacaggatgg
      181 agaacaagaa aatcaatttc tccactcgga ggtaatttca tccctaaatt tcgtgtcgcc
      241 gcacttaacc acgggagact atgaatcact gtgaaatcat cggacagacc ttcttttaac
      301 agtgtataga ctgttttttc cccataacta ttattatcaa tgggtccaaa tgccggaatc
      361 attttggcca tattactatt tccttataac atgtttctga caggaatctg catttgttac
      421 atagcccagt aattaaaaat aacttccgcc cattttaaaa attactcata cttattccga
      481 agcagactct gactaaccaa tatatacaat ctccctccac actctcatgc agcagccagc
      541 taacagtagt tgactgcctc aacttcaggg tgacttctgc cgttactttt ttaacccctc
      601 ccccagcgcc taaacgacac tttcgttttc tgcagtgcct cataggaagt atgagcttca
      661 aaactggtgt aatcaaaacc cgctgacgct accattctga caaatatgcc tgtgaatgat
      721 acccgacttt tccatcatga tgcctgttta gtctggcgcg caccgtacgg atgacatctt
      781 tcacccggcc agtttcatac aacgcatcgt aagctttgta cgcatcagcc tgcaactctc
      841 acccatattc tgccagatga cgttgtgaat cttcgaggga aagccgcttt atcatctgcg
      901 tattctgttc tatttgatca cagattggtc caatctgtga tcaattcaga tgtcattttg
      961 cattaatggc atcatcgctt ctggataaat aatggcaata aaatctgtca gatattaatc
     1021 tggttatcct gtttattatt tatcgtcttc ttacctgcat ctccatgtct cagaaaccag
     1081 tagcggttca acagttccgt cagggcatcc tgcgtcagtt tttgctgttg ttcactgcca
     1141 ttctggttgc ggacttcatt atagcgacgt aactgctctt ctgccgggat atgccggtta
     1201 aaagcctgca tgatgccaaa cacctctgtt ttcagttcac tgactgtcat gtattttccc
     1261 cgctgttcat ccagacggtt caagcgctca gccagctgct gtaggcgggt catgccttcg
     1321 ttccagcccg tcatcgcctc ttccgggagc gcacggctcc ttacactctt ctgccagtta
     1381 tccaccattt cctgtacccg gggattatcg gggaaaagta ccctcagttg ctgcagcagc
     1441 tgcgcactgc gctgcaggtt gtatgccagg ggtaattctg tcagtcgcgt tatctgttga
     1501 ccggaaaggg gtatccactc tgacgtatga tttaacagtg agcgtgttct gctgagctgt
     1561 gctatctggt ctgccgaaag cacatccggt gggggtgtca gcagcgccct gagttgctgc
     1621 tccgccggtg atggctgtgt cagaaactgc catcccggaa tggccacgct gacagccagc
     1681 aatgccgcac agacaccagc caaaaacggc ttcagaaaca tgggcgggga tttctgaagg
     1741 gagggtgtgg gctctgcttt tgtttcagaa acaacataca ccagacggga gtgcggcagg
     1801 gtctgatgtt cctgcacatc cggagcatca gaccggagtg cctgagccgg aagcgttacc
     1861 ggagacgacg cttcgccctg cacaggctga ttttccaggc gggtgatata tcccttcacc
     1921 atggcttccg gtaaagccac tttactgccc tgtttaagtt cgtgccgggc aagtgtgtca
     1981 ctcagtgcct tcaggaatgt ttcggtctga tacagcaggg gcaggttgtc acggtcgtcc
     2041 ggaggcatgg cccgaagcgt tttttgcaga cggttgaaca gaccggtaat aatttccagc
     2101 cgggcgtggg tattcagcgg ccacatcact gaccagtgat gccgggtcag cgccacaatc
     2161 agagccagcc cttcattcag tccacttaac cctgctgtat gcatccgggc gatggtgtac
     2221 caggatgccg tctgtaattc cacgccattg tgttcataca gacgcaggca gagcgtttcc
     2281 acgtaacgcc agtccacatc cggacgtgcc gggtgggtca gtttgctgat ttcatcacgc
     2341 agggcagcat aatccggtaa tgacctgggg tcaccaccgg ttttcagttt cacttcagaa
     2401 gtcatcggca gattcggtgt tactgtatta aagcatgatt aacagtgacg aaaagaatgt
     2461 cagtgtaata ccgggacccg ggaaaggtat tccacggcac ggatgattgc cggtgtcaca
     2521 tcccaccgcc gtttcagaac cggtaacagc tcattaaggt ttctgatata acgttcagtg
     2581 aacagccgtt gtccgtccct ggttaaatga aaaccggact gattacaggt aatggtgata
     2641 tgacaggtat cgctgccaat atagacagct tcctggggtc tgaatctgac attccagtgc
     2701 caggagtacg gagagtggtt cagataaaaa agacgcatct gaatatcatt aaataacggg
     2761 ttcatggcga attaaataaa cctgataata acagtatgat gaaagtccgg aataaagaaa
     2821 taatccggta ttatgcaaaa tcctcaaggt gacgactgat actgttttca cggtaacagg
     2881 tcaccagtaa ttcacgggtt gcccgggtga acgccacata aagcaccgga atcacttcag
     2941 actcatccgc tgcaccagag tgaacgaaag acgcattgat aaccgccaca tacgggaact
     3001 ccagcccctt gctgctctgg tacatcagta aatgcaccac atccttacgt cgcgagtatg
     3061 tcttcttgcc ttccctgtca aaacaggtgg caaaaggcac cccgcgtagt gtcataacgt
     3121 cctgcaattg ttgcgctgaa cgctctgtcg gacacagcac agccatatca ctccagtgac
     3181 cacacagggc atggcgcttc tccagccagt ctgcaacttg tctggcttca tcacgttcag
     3241 aagcacaacg caggatttcc ggctccgtac cctcttcacc gcccgcctgt ggctgaacga
     3301 gcggtatttc acggttctga tgtttttcaa aatactcgcg cgagaatgca taagcaaagt
     3361 gcagaatgcg tttcggatta cgataattga ccggcaggac agaagtgcgt ccctgtgcct
     3421 gaatccccac actggccaga gaaaagttca gggcccgctc ccgccggtaa atggactggg
     3481 cgtcatcgta catcaggagc agtgaacggg atgcgttatc aaacagacgg gcaatcaggg
     3541 ccagccagcg actgtcaaaa tcatgcccct catccaccag tacagcatca tatccggttc
     3601 gggtgatttt tccgctgttc acggcctctt ccagtgcact gaagcattta tcaggatatt
     3661 cttttctgtc ggccgtcacc ttaataccgt ggcgttttac cattgaggca caccagctgt
     3721 gaaaatggga taccgtaacc cgtgacgcca accctctggc ttcaatgcat tcccgcagat
     3781 agctggccag ggtaatgttg tagcacaaca ccagaatctt tccgggtgtg gtttcagcca
     3841 ggtaaaggca acggaacaaa agaatcatgg tttttccgga accggccaca ccatgaatga
     3901 cacgatgccc gtcaccaata tttctggcca gaatctcctg ctgaatatcc atgattttga
     3961 tttgtgagtt ctgtttcacg gtcacttcag gaaacagatg ggcgcgcaga atatctctca
     4021 tccggggtgt cacccgggtt ctgaatcccg tggtaaacat cccggcaatt ttttgctgga
     4081 atacctcagg taaaacagac tgcgtcattt catcctggca gattgtctgt gctgaaggaa
     4141 aaattttttc caccgcattc tcatcattcc ctgccagtgt cttcagttgc tgacgggtaa
     4201 tgcgggtaaa caccacacca tacgcccagg ccaggttcag ccgtccctta tactgaccgt
     4261 cattctgccg taagcacggg tttgcaggta aggcattaac cgtatcacag gcataacgac
     4321 gaacctgtac cagcggattg atttctgatt tcagaagacc gtcggtttcc agcgtgacct
     4381 gctcctgatt tacctggcgc agcgtggaaa ccgtccagtc cttgacctcc agaaatacca
     4441 gtccgttctc cgggtcaata atgacaaaat ccggatgccg gtttttccgt cccaccggaa
     4501 tatcgtacca gacaaggcag tcatcattca gaaagctctc cagtcgtgaa gccaccctcc
     4561 tctccccagg cgtcattctc tcctcagtgg aggccgatac atttatcagt ttcgccataa
     4621 acgcacctta atctgtgaac aaaaaataat caccaccgac atccacggat gttatatcgt
     4681 gggtgtcgct ttattcagaa ataaaaaaca gaatcattaa catgaccaga atactgtgaa
     4741 ccatgctgca gggtatctgc agatatccct tctgttatat ccggccgggg taaaatcaga
     4801 acactgactg tcaggctgct tcattttctg cgtattccag actctgcagg tactgcagcg
     4861 ccgccgggtc agccacatat tccatctgcc cgtggtgacg ctccacttca ttaatgattt
     4921 tctcaagctt cacgcggaaa aattctttac gcaggttaat cctgttaatg cggtattttt
     4981 ccagactgtc atgaagtgtt ttttccagcg caggcgcatc atcacaggaa atcatggcgt
     5041 gaacatcaaa atcaaacgga acagaagcac cacttaactc tttgaccctg tccatgggtt
     5101 ccagccgtcg ggtcatgcca attttaaaga catcctcacc aaacgaacca atattcgaaa
     5161 tgatataaac atgcccctgt ttcgtaagct gagccatgga tttagcacgt tcatactgcg
     5221 catgtgcttc ctgaattttc tgttccagct ccaggcgttg tttctctaac tcctccctgt
     5281 gagccccttc tgctgcgcgc agcgcctcct caatcagttt ctgctgctca gcaagacgcc
     5341 gttcttcctc ttccgcttcc cgctggcgcc ggtcaagttc atcctgacgc tctttttctt
     5401 cacgtatctg acgcttaagc tctgcctgat attccttctc ttcctgcgct ttttccagca
     5461 atgccacttt gtccagaaac tgttgcctga cactctccca gtcagaatta tcttccaggt
     5521 taagaaaggc tattttctca ctgacagcag cgtaagcctc tctttcctta cgaagctggt
     5581 catatttgct ttgccagtta ttaatcgtta caccagccag cagagtcttc gccttaaatt
     5641 tccaggcgga atcaacaatc cgttgcattg cacggctggc cttctgtctg gcggtatgaa
     5701 attcctcacg acgtctggcc agttgctcct tttcctgtcg tattccccgg gctatcacgg
     5761 cattgacagt ttcattaaaa ccccgctctc tctgaaccag ctctgaaacg gcctgctcaa
     5821 aggctgctgt tttctttctg agtctccggt aaataaaaaa acgaagtaaa attgcaaata
     5881 cagtaagcaa accaaaaaac aaaccaataa tacctgccgg actcgatact gcatcaataa
     5941 agcgctcatt aagatttatc agcgccctga ccgccacata aatcccccag agaataacgc
     6001 aaactaaaat caggacaaat cccgctgcat tatcatcttt tttcttcctg ccatgacttt
     6061 cctttatttt ccttctcagt aactgaatat ctttgattaa cgccctccgg aaatatttat
     6121 acccggaagg ctgtccagct ttatgatacc cgccagtaca gaattaactg caggctgagc
     6181 acagatttct ctgctatttc agtgtcagat taatactgat taccggaaca aatgggcaga
     6241 tgtgtaaatc caggtttgct ggccatataa agcggcgcac aggtgggttt acggggttca
     6301 gtgaccggta caacctcatt tttcccggcc accgacggtt gtgacacctt ccggacagcc
     6361 gctgattttt tcttctgaac cagcttcgtg gtgaccggtg atttcacctg acgtttggcg
     6421 ggtgccacgc cataaccgga acaaaagcgc ttcgactgac tcagacttcc gtcattgcag
     6481 acaaaacgtc cgtctgacgt gcagtgtgaa ataccacctt tagcacccga gcagggtttt
     6541 cttccccgcg cagcatctgt ctgaaccgaa aataatctat ggtcgccccc gtttttgcaa
     6601 cactgatttt ttaattaatg gtgggcttgc ttaaatctat ccggcgtctc tgtaggcaag
     6661 gatgcctgcg ccccgatgag tttcgcacct gataatccta aaaagcccct cggcttcagt
     6721 gagccatttt atttattagg tttttaatca ggtcgatttg ccgttcatgt catcaataat
     6781 cagtcttcgc aaaaccagac aggcaacttt gtatcttatt gtttacaata agccaattga
     6841 gttatatcag catttatatc aagcctgata ttctttttgc tgagtagtga ttacccaggc
     6901 aattcgagcc agtttatttg ccagagcgca ggcaaccaca ttcgaatgct tcttgttgag
     6961 ttgctcccga acccattcgg caagttttcc atgctggcgt tcaagccgca tcatgaatga
     7021 gcgggcacac tgaaccagta accggcgcaa atttttatcg cctcgcttac tgatacccaa
     7081 cagagtcgat tttcctcccg tgctatactg ccggggaacg agccctgtgg aagccgcaaa
     7141 atctctgctg catgaaaact gtttcccgtc accaggttgc gacgatagta agctggcagt
     7201 aatcggccca acaccaggaa ttgtcataat gcgctgagcg gtatcatcag cgttgattga
     7261 ctgacttaat tctgattcca gctctgctat ttgctcaaca aggtaaaggt agtgggtatg
     7321 caacctcacc aaaaggcggc tcagatattc ggggatttcg tgttcagcca acacgagaga
     7381 caaccgtttt attacagcat cacctgtcgg caggctgatt ccaaattcca gcaagaaacc
     7441 gtggatctgg ttggttgttt tcaccttatc tctgatgagt gattctcgga cccggtgtaa
     7501 cgccctcatg gcctgcaggg gctcagttct tggctggaca aaacgcattg atggtcgtga
     7561 agccgcttcg catatggctt ctgcatcaac aaaatcattc ttattacttt tgacaaacgg
     7621 gcgaacgaac tgtggagaaa ttagcttagt ttcatgccca aaatcagaga tacgacgagc
     7681 cataaagtgt gcacccgcac aggcttccat tacaacaact gagggcggac atgtcgccag
     7741 aaactgcatc agtttagtac gggtaaattt tttacgcaga agagccttac caaacttgtc
     7801 ttgacaatga atgtggaagg agtgctttcc gagatcgata ccaataagtg tgatgttctg
     7861 catgatggtc tctccgttaa agggattcac cttaacagcg tactgctgtt aaggtggggg
     7921 tgaccatctc attagtccca cgaacagaaa acagataatt ttcttcattt aaaaactcat
     7981 acattgttat tgctgatatg ccagagttat caggatgatg aaacattccg ccagcgttgt
     8041 cataagccgg aataatgtca cattcacacc tgacatatat ccatcactgt ttatttctgt
     8101 tctgccctcc ctgctccggc tcacgtctga acgtaccgct gctgtttcag atggcgcaac
     8161 aacaccctgc cctccggtat aaatgttgtt ccgtaacgaa ccagcccggc atctttaagt
     8221 tctgcatcaa tgacataatg caactcacca ggctgagtct gctcctgcat cgtcacgttg
     8281 acacgcttaa tacgtggctc atacttcagc agaacatctg aaagcacacg catcagttga
     8341 tgcgccgttc ccggcattcc ctgcagaacg gttgtcatat ccggcagacc ataatccggc
     8401 agatgtttaa ggcttccggc ccgggtattc agaatacgct gcatgttatc gagcaccgaa
     8461 agaataacct gctcttccgc actgacctgg tttaactcca gcccgccagc gaaattgcca
     8521 tagagaattt cataaaggga aggacgcggc atcactattt atccttcacc ggtaacagcg
     8581 tcagggtatt accggatact tcaataagac ggggcgtgtc cggctcaagc tcatcccggc
     8641 tcagcaccac ccgccaggta tttttcttcc ggtcaggctc cagaaacatt gctgccacac
     8701 cggtaaattt cgccgcatca tccagaggca tatccacggt tactgagcca cctggctgca
     8761 tccatacatc tttctgtgca atgatatcgc ctgccagaat ttcactgtcc cccgtaaaaa
     8821 gcgcctgata atccgcgctg tcaaaattcc ggttgtcttt aagctgataa atccgcacca
     8881 caaccgacag gggaataccg gcagcactgg tgttaatggc ctccctggca cgaatatcaa
     8941 ggtgaagtgt ttttatctgt cggtaaaaca gggatttcgt ggcagatacc gtaccgtctg
     9001 ccactctttg cgtcaggcca caccctgata acgacaggga aaacaacgca ataatcagac
     9061 cgtaaccagc cttaccagcg ataatcgcca ttttcctgag cctccttacg ttgataatgt
     9121 tcctgaaccc gctgataacg ccccagacgg atggtcatta cgcgtgccgc tgaagtcgtt
     9181 atctttgcct gtgttctcat tacagccgtg cgccccagtt gcggactacc ggctgccggt
     9241 cggcaactga gccggacatc cggcaacagt gaacgctcaa cacaaagttg cagacgtaca
     9301 tccagacggc ttcccagata aacgtgcaac aacgccagca aatcagaata cagctgcccg
     9361 cccggtaacc agccctgtac ctcgctgccc gtctgtgttg aaagctgtaa taacacctgt
     9421 ccgttcacat ccgtagcatg atcgcccatc accgggcgac tcttcagact gaccggatga
     9481 tgaacacgca tggccagcgg tgttttcagc gggatccgtc gtcggtcatg atgccagaca
     9541 cgggcctgtg ttccgggagc cagcagcgtt accagtgatg tcagcccttc agctgttctt
     9601 ccgggtaaca acatcagggg caataacgcc agaaaacgcg ataccggcgt ggcaatattc
     9661 tgtgcacatc cggggatccc cagtctggcc agaccgagca gatactggga tgttttatcc
     9721 tgcccccccg cctcaaaggt ggccgggtac gaatatttgc gccagatacg gtaatactgg
     9781 gtgatcagac gatgactgaa tatatcgaga aaatccgctg cggcttcatg gccttcccgc
     9841 tgctgtgcaa tatcactgat atagtgagcc ggcaacggag acgtgacccc gtaaagtccc
     9901 ataaacgtca catgtacagt gggtggcaga tgggattctt caggaattac agcatctttt
     9961 atttcactcg caggaaaccc cattcccgtg tatggacaaa agcgaaccgc ttcctgacgg
    10021 gcctgccagc cactgccaat gggaggctga tctggctggc tttgttccag taactgacag
    10081 aagcgataaa aattaatata cggaagctgt ttccggtaac gggtaattaa ccgggcaggc
    10141 gcggattgtg attttctttc caccggatgc attttccttc aggctgaaca atcagtgtga
    10201 gctgattgaa ctgattcata tcggcgtaca gggccaggaa acggttgagc atttcgccaa
    10261 acaggtgaat atctccctct ccggcaaacc cgttgccatc aagagttacc tccacatcaa
    10321 gcccccggag cagaaaaccc ttttcaaaac gctgaatacg atgatgctgt acagccagaa
    10381 tggcatcaag ccgacggtga ttaagttcat cgtcccgcca gttataaagc gccagcgtgc
    10441 cacgcagcac ttccgcactg ctcagcatgt taagaaatcc tgtccccaga tgactcatca
    10501 cccgccagtg aaaacgatct tctgtcggcg gataaaccgg cagcgtgggt ttacaaagat
    10561 tacatacact gatcggggtc tgcagtacct gttcacaccg atcaagcagc gtactctgca
    10621 acgcacgacg gggaagctga ccattggtac ctgtaatacg taatgaaagc gtttcccgct
    10681 ccggcatccg atccgcctcc caccgctgac cgccaaggat aagccatgtg tcatacatcc
    10741 ccgtgacacc ccgcttcacc cgggtatgat aataacgttc gggtgcatca tggcgcagca
    10801 ttcccccacg atgacggaag ctggtaaaag gcacgtactc tgcactgccg gttcgcccgg
    10861 agcccgccac cgcatccact gaatatatct ccgtatatcc atcctgcaga cgcttcgggc
    10921 gcagcagata ttcgctctcc agcccattga tgatgagtgg atcggcttca agcgtaaaaa
    10981 ggttaatcac cggcacacaa tgcagacaca gggcatcatc tgtcacaggt aaatctgccg
    11041 gccaggactg actcagcacc acctcaatat caaatccgga aatacctgcc ggcaaagaaa
    11101 tattctccag tccgttcaga tgaacaaaca taaatttttc ccgaaaggtg aaatattcca
    11161 gcaataactg atacccactg aaagcactgt cacctttcgg ccacagccga tcttcttccg
    11221 caaaaccacc gggtgaaaac catccgtcaa tgcggatccg ctcatcattt cccggcagcc
    11281 tcagatacag cgcagccaga cgctttgtca tcatcagatg aagggtgctg ctgaccgggg
    11341 cttctgctgc cagatacagg ctcagacgat gcaaatcgac ctgagaccag tcagccagtt
    11401 cactgcaggc aaaacctatt ttcagcactg agcgcccgtc cggctccgta gtcataacga
    11461 ccttctccac tgccagtgga ttcagtggta tggcccgggt ggtacggtaa cgacagactg
    11521 tatttccggg gccaacaggg cgggaagtca cttccagtcc ggctggtacg gtttcagcca
    11581 ttttcatgac ggataaccgg ggagtcagtg ccaccaccga cagggacggg atagtacgca
    11641 ggtaatgagg ccagagcata ctgaccagac tttcagtaag ctccggcaaa tcatcatcga
    11701 ttttctggcg cagccgtccc atggaaaagg cgaaaccttc aaacaggcgt tccacgcagg
    11761 gatcaggcgt acccgcttta tcgagatcca gcatcgccgc ccgatcggga tgagcctgtg
    11821 caaacgcctt tccggcttca cgcagataac gcatttcagc gtcaaaataa cgcagggtta
    11881 aatcgtccat tatcttctca gaataaagct aactgttccg gagactcgcg ctctgacttc
    11941 agccatattt caaacaatcg cccgaactga actgcaggca ggatttcatt aatcagatta
    12001 cacatgaata taattcagac atctcatgaa cttcccgaat tgcacaacac agcagctctg
    12061 gcagcatcaa tggcgatgag cccggcaaga agatgttcca tatcaggctg cagacgcacc
    12121 ctgtcacttt cagtttttgc ggacttcata cgtaacagtt tcaggcggcg ggcttttacc
    12181 tcaaatacca gttcaggttc ccattgtgac agggtcagcc gggttgcacg ttcatccagc
    12241 tccacaagca gatgcagggc aaggtcattt tttccagtct gttcggcaac acgggccatc
    12301 aacaggcgca gtagccagtt gctgcggtca gactgaatac cgggacgtgc ctgtaaccag
    12361 cttaaagcgg cttctgttcc ttcattatcc gcaatctgca gggcttccgg ctcaagagat
    12421 aaaatgtcgt tatctccggg agctgccgtt atcgttacag aaggttcttc cgcccagcgt
    12481 tcaccacact ccatcacctg ctgctgtatc cagctcagcg tcacgtcatc ggcaaacggc
    12541 atgccgtcgt taaaagccag tgtctcaagc cccggcaggc gcagaagcaa tcctttcagg
    12601 tcatactgaa taatggcagc ataattttct tttccggtct gcaacagcgc ctgatgaata
    12661 taccactgca ggtccagcca cagatgactg gctccccggg caaacatatc gtcacactgt
    12721 tcaagcagtg acaaccagtt ctgctgcaga tacaaccgtt tcagtgacgc acgccggtcc
    12781 ggccccggtg gggcaatacg ggtccgtcca tcagcagata acggcggtaa ctgatgcagc
    12841 gtgtcatgcc ggacgctttt catcagtcga tgagccgcca gccagccctc aggctggtca
    12901 cgcagatatt ttgccagaac acgggcctgt gacagcagtt cctgtccgga ggtgatggca
    12961 gataatgcca tgacgctgcc ggatgttacc gtcggtgccg gcgcggcagc ctcctgaggc
    13021 accactgcat caacaccacc ggccttcatc aggcgatttt caagtgcctg atataacccg
    13081 ttcagcaccg gggcggaagc ctcatccgta aaggtctgtt ctgccagcca cagcgccccg
    13141 gcaatgcgca gggtatccgc cttcaccacc tccggataca gagacagact gtccagtatg
    13201 cgggcgctgc ataaccactc cagcgccgcc ttcctggccc gtgaccgttg tggatgcagg
    13261 tgctctccaa aacgctgcaa caaaccggtc agcagctcca gaccgtcggc cagccctttt
    13321 tcaccatcct gccgcagacg ggcccagata taccaggtcg ccacccggat atttttggtt
    13381 ctggtggtca gtaatttttc tgcaagctgg ctgacaattc cggcatcggc tccggagagt
    13441 ttattgacct cttcacgcat ctgctgaaaa tcatcatcgt accccgcatc atcacctgcg
    13501 ggattatctg gcgtcacggg agcaagccat ttatcccaca gcgcaatacg cacctgagcc
    13561 ggttcagcca catcccgggg tgtcgtctgg catgcactga ggagcgaatg aatggaagcc
    13621 atcagtcagt ctcctccacg gaatccccgg catcttcgtc attgcctgta aacgctgacg
    13681 taccggagag ttcgaaaacc gtttccggca gaacaaaatt gcggagtttc agcaatacaa
    13741 gcggcccttc ccccgcttca gttcgcagtg tgtaattcag catacgaccg tcctgcgcct
    13801 gccagctcag gctccagcca ctggcaacgc ccggtgcggc tttccgtcgg gccatctcaa
    13861 gcagacgtat cagcccccag cttcccggca ggtctgcata ctgacgggta ccggcctgag
    13921 tgcttaccca gcttaaactg gcgccaggtg cttccgtatc agccggccag gtaaatcgct
    13981 tccataccgg catctgatta acataaatca gtttctgatt atcggttatc agcgtcgtct
    14041 gcatcacacc ggcagcagtt cccgggcgca gctcaaaatg cagccccgcg ttacctgtgg
    14101 tgaacgccac atccgctatt tcactcaggg tattgatggc tttcagaaac gcagggttga
    14161 atgtcagtcc ccgggtatta atggtatcag gtacccagcg actgccttca ctgtgcaaca
    14221 caccactcag gttattctgc aggaaacggg caatacgtcc cgtgtccgta ttcaggtatt
    14281 ttgccagcag gggaagcgaa gcatcgctgc tgacattttt aaacggataa cgcccggaaa
    14341 atgcattatt ccagccatcc accaccgcag tacgccaccg ggcattcaga ctttctgccg
    14401 cgggcgtaag aacctgctgc cacgcctgct ccatcgggcg aacaaagacc gtctgtccga
    14461 agccatacca ctcctgtccg agtccggcag cggtcaggct accgtaatcc cgggtatcgg
    14521 ttaaatcaac cgatttcccc tgcaatactg tctgtgccag catctgcatc atggcctgcg
    14581 ggtcagaaga gccggcaatc tgttgcagac gcagacgaac ctgtgtcacc cgggtcagat
    14641 atgtctgcag attcagcata tccgcactgt tgttctgatt atccatcagt gcaagcaccg
    14701 gcccgaaagt cgtcgccaga ggaccgtgaa gccggctttc cggtactgcc actggctgtt
    14761 tttcctgtga aagcaggttt ctggcagatt taaccagtga atcagtaacc gcttcccggg
    14821 gctggccagt acgcccctga accgccagcg tattcatcag agcaaccagg ggggactggc
    14881 gcacatcagc catcaacgtc agttgctctg tcacatcaga cagcgtctga gctttacgca
    14941 agtgcagact gttcagaaaa ttcagccagg cattaccaaa atcagcaaaa tagcgcgttg
    15001 tcaggcgctg tctgagtgct tccggcgata ccggtgacgg tgccttctgc ctgccatccg
    15061 tcagtaccca gtccatttct tcacgacgct cattaatcac ggtgtctatc gacggcaata
    15121 cggcctcttc ccaggcctgg cgggtaaaca taccgggaac cacttcatcc gtaaaaaaga
    15181 ggcgggagac atcagtatca ccggtcatat ccgtcagacg catatccgca aactgatgtg
    15241 ccacccgtgc cagcattttc tgatacagca tggcatcgct gttctgtgtt cccagatggc
    15301 gaagcagcag agttctgctc tggctgacca gctcttcatc agccgtcagc ttccactgag
    15361 gatgagtaat aatcccggag gcataatacg ccagcaacgt cgggccatta gcctgccaga
    15421 aaacggcact cacaccgtca cgctgtggcc attcccgcat gagcgtacga ctgaacctgg
    15481 cgggctccat atgttgcggt tgcgccagca tcagatacag tctgagctgg tcatatgcgg
    15541 cttttgccat tttcccccgc tgcgggctgt ccggaggaag acgaataaat gtcctgagct
    15601 gctgctcaag atgagcagca gcggcatcac gcagtaaaag atgtgcattt tgtgcataaa
    15661 ggggcattac gaccgcgagc aggtctgcat tctgattcat accggcccgc aggtaccatg
    15721 gcgccccgtg ctcctcccgg tactgaaggc gggccaggga tttctgcaga ttcagcaacg
    15781 cctgcagacg ttctgcagga gacagttgct ggttctgagc cggacgaatc tgttcctgta
    15841 cttcagcaac cagactccgg tttgccagaa aggaaaccac cattcctgtt ccctgagcca
    15901 tcatcagtat ggccgccata acggccagac ttctgcgcca gttatgacct gtgcgggaag
    15961 gctgtagccg gacaggtaat tgctgcactg tctccggcaa cgcctcccag cggttatcca
    16021 ttcgccagcg atgtctgaca cttcgttcac cgccgacagt tgccggactg aaaacaatac
    16081 ccgccagcaa caactgacgg tatggacgga gtaatacgga taatggctcc accacagcat
    16141 caatatttcg tctgaatcgc tctgccagcg acaacaaaaa ataataccga ggggcacagc
    16201 atatctgctg tatcccctga gcaacaagcc cggggagcat ggcctgtagt tgtgctttca
    16261 gtttgtcaga actacattct gctggcagaa ggcatcccac gggctgagta atacgccctc
    16321 gctcatccgg actctcctgc agtgaccaga catacagcgg cagacgccag ccaaggcgct
    16381 cacaacatga actgaccgca cgggaaaccc tgtcaagggc atcttctgtc agtggtgcgg
    16441 ataatgtttc actcagtccg gaagttaccc agacaatgcc atcagcaggc cggtaacgca
    16501 gacggcgtaa cgcagccagc cagtctgcgt tttccggctg cgaggggtct cctccccaga
    16561 gaagcaatgt cccctgctct tcctgccaga actgttctgt caggcccggg gtcagcaact
    16621 caacctcaga ggcagtacct gttatcaaca gaatgcgtat tttacgtccc cagaatctgc
    16681 catgctggcg ccgcagagta tgacgaatat cctgaacagt aacatagcgc ggtgacgtct
    16741 gagccagtcg ctcttcatcg tcaggtaacc tttgctcctt acgggcccga cgtcgatatg
    16801 tcagttcctg aacatatttt ttcatcacag gcaggtaaca gacaaagagc aaccccagac
    16861 tcagcaatat cagcagtagt atttgatccc ggcgtgtttc tgcccccaga aatgctccat
    16921 atcgggttat cagccaccag agcgcagcca caccaataaa tacaatcccc ggtcgtccaa
    16981 agcgaccaga cagacaggcc agtttattca ttctggtttc ctgatgtaac agtactgata
    17041 aaatgttctt ctccggatga gaataaagtc aggagagaat cattaccaaa acagaccaga
    17101 tcggctgcca gagcagtaag cagccacgga gcagcaggtc ccgggatacc gcaccatttt
    17161 tccagtaaca tacgtccgga cggttcccat ccggcaccat actggtttcc aatagtcatc
    17221 agcggtgcag caatcttttc ccagtgataa cagtccccca gtacacgagc ggtacgacag
    17281 gcagctgtct gggtttcaag gaaaagtgtg aattcatcat taaacttgtt gatatccagc
    17341 gacatcgggc gtagcagacg ggctggatgc ggcaggttat atttttgcgc aacatcatcg
    17401 ctggtcaaca aaagtgcagc cagtccgtca gagtatgcat ttcccccatt aagctgtatt
    17461 accagtatca aatccacagt cagcactggt tgtttcagac gctcatcaac ccaccccatg
    17521 gaaaagtcgg gagtaacctc gatattgtca ggaacaacac gttggggaaa taacacttcc
    17581 catgcagaaa caaaagcaga tctgatttca gaatcaggct gatcggttat cagcgtcaca
    17641 tttacaggga gaacggcagg taactgacaa aatttatcca ttatctccct aagcaagaca
    17701 cgtaaagagg cttcaacctt atgcccactg tcagaaagat aatctatttt ctttacaagg
    17761 ccgtactgca gaggcaattc atcacaaaga gtagctaccg ttattttatc aggcaaataa
    17821 acacaactgg cagtaataac aagatacctt tcggcccagg attcccactg tttctgagca
    17881 tactccccct cttttttaaa gaacaaatgt tcatccatct ggttgctcca ctgccagtgt
    17941 cgcaggcaaa acaggaaaaa ccagcccccc ggaggagtca atgcaagcca ccagatactg
    18001 aaatcattta atgctttcac atattctgat gcccggataa taaacaacag agcaccaaca
    18061 acagccataa atgcccctgt cataaaccac aacatcattg atggtttatg aggtaattct
    18121 gttgataatg attttgttct gctccatccc atgggttatc ctattgcaca ttcaggtgca
    18181 ctggatataa ccttacaccc acacagacat ttacatccat caatcacaac agctcttcca
    18241 tcagatatcc attcaggaga gccctccaca ataggattag taccatgtcc ggaaacagga
    18301 caactaacca tatctccgac aagagctact tttttaccat cgacaatcat ggttgaagat
    18361 gccgaaataa ctttaccacc atgtgtggta ttatcaccaa gcaatacaaa acctttagac
    18421 ataaaactct caaaaataaa ttttactttg agtcaccagt taccgtaggg gtaaattttc
    18481 cctttgataa aatctggtgt atcaccgtaa taaacatatc catctggatg tttagtaacc
    18541 cccttacaca tattcatttt atcacccgat actgtagtaa ttttagcatc caactgaggt
    18601 accaccggat taccgcgatc tcccagccag actcttacct tcccttcagg tgccagacca
    18661 ataatcatcg ttgaacgata ataggtttcg ttccagcggt tgtctgtgta aggcgtaacc
    18721 atctgctccc aggtcccttt tttaaaaaaa atcagagttt cataactctt tttatcaatt
    18781 attgaatccc agcaaaaacg taacatcaga ggtaatgctt tccccttgtt ataatacgac
    18841 tcagtcgcgc ccagatggtc attccagcgc cctacactga cctgactcgg tcttgtggta
    18901 tcaagatact gataagtcgt cttataacct tccccgtcca ccatcaatgc ctgagtggcc
    18961 agcgcaggca gagcctttgg ataaataaag agaaatttcc attcatcata gggtaaattt
    19021 ttataacccc attcagtctt atccgaatca gcatactgtg agctaaccga attacaccca
    19081 gttaacaatg ccagtaatgg tgcgataaa
//
LOCUS       DAZUCC010000087        19101 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZUCC010000087 DAZUCC010000000
VERSION     DAZUCC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.087"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            208..936
                     /gene="mpaA"
                     /locus_tag="ACHY09_002605"
     CDS             208..936
                     /gene="mpaA"
                     /locus_tag="ACHY09_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFS3885560.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(911..1876)
                     /gene="ycjG"
                     /locus_tag="ACHY09_002606"
     CDS             complement(911..1876)
                     /gene="ycjG"
                     /locus_tag="ACHY09_002606"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFS3885561.1"
                     /translation="MRTVKVFEEGWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQESLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1995..2501
                     /gene="tpx"
                     /locus_tag="ACHY09_002607"
     CDS             1995..2501
                     /gene="tpx"
                     /locus_tag="ACHY09_002607"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFS3885562.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2545..4086)
                     /gene="tyrR"
                     /locus_tag="ACHY09_002608"
     CDS             complement(2545..4086)
                     /gene="tyrR"
                     /locus_tag="ACHY09_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFS3885563.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4234..5295)
                     /locus_tag="ACHY09_002609"
     CDS             complement(4234..5295)
                     /locus_tag="ACHY09_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFS3885564.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIEDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5292..6689)
                     /locus_tag="ACHY09_002610"
     CDS             complement(5292..6689)
                     /locus_tag="ACHY09_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFS3885565.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            6844..7842
                     /locus_tag="ACHY09_002611"
     CDS             6844..7842
                     /locus_tag="ACHY09_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS3885566.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGNQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLIAGKPSPQNITVATHMSLK"
     gene            complement(7893..8798)
                     /gene="ompG"
                     /locus_tag="ACHY09_002612"
     CDS             complement(7893..8798)
                     /gene="ompG"
                     /locus_tag="ACHY09_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFS3885567.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(8843..9925)
                     /locus_tag="ACHY09_002613"
     CDS             complement(8843..9925)
                     /locus_tag="ACHY09_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3885568.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFLNYALYPHMTVYDNMAFGLK
                     MQKIANEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTISEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(9939..10598)
                     /gene="pgmB"
                     /locus_tag="ACHY09_002614"
     CDS             complement(9939..10598)
                     /gene="pgmB"
                     /locus_tag="ACHY09_002614"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS3885569.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     ADLRAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(10595..12862)
                     /gene="ycjT"
                     /locus_tag="ACHY09_002615"
     CDS             complement(10595..12862)
                     /gene="ycjT"
                     /locus_tag="ACHY09_002615"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFS3885570.1"
                     /translation="MIRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWCTSNGAGYTITSRRFVSADQLPLIALEITITPLDADALVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKRVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQATGDESFIAHEGMALLLGTAK
                     FWISRAVQVNDRLEIHNVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARKFGCSDDA
                     FIHRAEIFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRNGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(12859..13914)
                     /locus_tag="ACHY09_002616"
     CDS             complement(12859..13914)
                     /locus_tag="ACHY09_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS3885571.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(13924..14712)
                     /locus_tag="ACHY09_002617"
     CDS             complement(13924..14712)
                     /locus_tag="ACHY09_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS3885572.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVITACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEKVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALLEQLRADNFQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(14731..15783)
                     /locus_tag="ACHY09_002618"
     CDS             complement(14731..15783)
                     /locus_tag="ACHY09_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS3885573.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCEIARRHGADFCLNPIGSDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(15814..16656)
                     /locus_tag="ACHY09_002619"
     CDS             complement(15814..16656)
                     /locus_tag="ACHY09_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS3885574.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(16643..17524)
                     /locus_tag="ACHY09_002620"
     CDS             complement(16643..17524)
                     /locus_tag="ACHY09_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS3885575.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(17545..18837)
                     /locus_tag="ACHY09_002621"
     CDS             complement(17545..18837)
                     /locus_tag="ACHY09_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS3885576.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITVGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQTSQKKLDELVEQR"
     gene            complement(18851..>19101)
                     /locus_tag="ACHY09_002622"
     CDS             complement(18851..>19101)
                     /locus_tag="ACHY09_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HFS3885577.1"
                     /translation="TLRRSHKEFHPDNNFTIDAINSFVMRIQRSNADGDCLTGLFNVS
                     ENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
BASE COUNT         4585 a         5306 c         4660 g         4550 t
ORIGIN      
        1 gattaacatg tgtagaacca gtaagaataa aatacgccat gtatataaca ttacgacaat
       61 tctaatatct gcacatcaat gaaaatgcaa acaacttccg gcaaaaaagt gatacaagat
      121 cgataacata gcgttgaaga aaaggatttg ataatccgca aaatttgcac aacactgcct
      181 gtaatacttc ctgaaccaga gacgatcatg accgtaactc gcccacgcgc cgaacgcggc
      241 gcatttccgc ccggaacaga acattacgga cgttcattat tgggcgcgcc gttaatctgg
      301 tttccggccc ctgcagccag tcacgaaagt ggtttgattc tggctggcac tcacggtgat
      361 gaaaactctt cgatcgtcac gctctcctgt gctctgcgga cgttgacacc ttctttgcgc
      421 cgtcatcatg tggtgctgtg tgtgaatcct gacggctgcc agttggggtt aagggccaat
      481 gctaatggtg tggatttaaa cagaaacttt ccggcggcaa actggaagga aggtgaaacc
      541 gtttatcgct ggaacagcgc cgctgaagaa cgtgatgtcg ttttactgac aggtgacaaa
      601 cctggctccg aacctgaaac ccaggcgctg tgccagctta tacaccgcat tcaacccgcc
      661 tgggtggtct cattccacga tccgctggcc tgtattgaag atcccagaca cagcgaatta
      721 ggtgaatggc tggcccaggc gtttgaattg cctctggtaa ccagcgtcgg ttatgaaacg
      781 cctggctctt tcggcagttg gtgtgcggat ctcaacctgc attgcatcac cgcagagttt
      841 ccacccatct cctccgacga agccagcgaa aaatatctct ttgcgatggc aaacttgctg
      901 cgctggcatc ctaaagatgc aattcgcccg ttgtgaactg aagcgccggt tccacatcta
      961 ccgccagcca ggtcggtccg tcaagatcgg cgaaactgac ctgcggcacc agcggtaaag
     1021 cggcgctaat ggcacgagag gtacacaaca tgcagcccag catcagacta aaaccttgtg
     1081 cacgtgcttc agtcgccagc gccagcgctt ccgtcaggcc ccccgtttta tcgagcttaa
     1141 tgttaaccat ctcatagcgc cctttcagcg ccttcagatt gctacgagta tgacaacttt
     1201 catcagcaca aatcggtaac ggatgaataa aattctccaa cgccgcatcg tcctgcgcag
     1261 gaagcggttg ttcaagcatt gcaacgccta aatccgccaa tagctggcaa cgcgccgcca
     1321 acccttctgc acgccaggat tcatttgcat caacgatcag cgtcgcatcg ggcacagcgg
     1381 tgcgaattgc caccatccgc tcactgataa gatggttatc cagcttcact ttcagtaatt
     1441 tcgcgcctgc ctgccagagt gttgatgcac tattggccat ctgatcaggc gtaccgatga
     1501 caaccgtctg tgcagtgata actgtcccgg gaagcgttat gccgatcaaa tcagccagcg
     1561 attcctgctg ttttcgcgcc gcgagatccc acaatgcaca atccagcgca ttacgtgctg
     1621 cgccggcagg gaggattttt tgcaactcct cccgtgtcag ccctttctct aattgcggca
     1681 cgacgctcat aatttgcgcc attaccgagg catcactttc tccataacgc ggatacggcg
     1741 tgcattcacc ggtgccttta ataccttctt cttccagttc aaccaccacc acgcgcgctt
     1801 cactgcgact tccccgagca atcacaaacg gggtatgtaa gggccagcct tcctcgaata
     1861 ccttaacggt tctcatcttt actccttgtt gtcgcggtgg tctgcggatt aagcgcaaat
     1921 agcgtttgct gtgttattga cagttagcat aaactaggtg tgacgttaac tatatgtaaa
     1981 caggaaagat aatcatgtca caaaccgttc atttccaggg caacccggtt acagtcgcca
     2041 attccatccc gcaggcgggt agcaaagcgc agacttttac tctcgtggca aaagatctgt
     2101 ctgacgtcac cctcggtcag tttgcgggta aacgcaaagt gctgaacatt ttcccgagta
     2161 ttgataccgg tgtttgcgcc gcatcagtac gtaagtttaa ccaactggca acagagatcg
     2221 acaacaccgt tgtgctgtgt atctctgccg atctgccgtt cgcccagtct cgtttctgcg
     2281 gcgcagaagg tctgaacaac gttatcaccc tctccacttt ccgtaacgct gaatttctgc
     2341 aagcctacgg tgtggcaatt gctgatggcc cactgaaagg tctggcagcg cgtgccgttg
     2401 tggttattga tgaaaatgac aatgtgattt tcagccagct ggtggatgaa atcaccaccg
     2461 agccggatta cgaagcagct ctggctgtac tgaaagcata agttaatatg cctgatggtg
     2521 ttgcaccatc aggcatattc gcgcttactc ttcgttcttc ttctgactca gaccgtattc
     2581 ccgcaactta ttggcaatcg cggtatgtga aacgccgaga cgttttgcca gtttgcgcgt
     2641 gctgggataa ttgcgataaa gctgggttaa taccgagcgt tcaaaacgac tggtgatttc
     2701 gtccagcgaa ccttccatcg catcttcgcc cacggctacc gttgcggcgt cataatccgg
     2761 caacaaaata tcctgtggac gcagctcata accgtccagt tgtgtcagtg cgcgatagat
     2821 agcgttcttt aactgccgca catttcccgg ccacgcataa cgtgtaagta cagtattcag
     2881 gtcagcggcc agtttcggac gcggcacgcc ctgctcgtcg gcaaagcggg cgacgaacag
     2941 ctcggttaac ggcatgatgt cctgcggaca gtcacgtagc ggcggcagat tgagcgtcaa
     3001 cacgttcaga cgataataga gatcttcacg gaacacgcct ttttgcacca gttcgaccaa
     3061 attcttctgc gtagcgcaaa tcacccttac atcgacatgc acttcatgat cttcaccaac
     3121 tcgacggaaa gtaccatcat tgaggaaacg cagtaatttc gcctgcatcc ttggcgacat
     3181 ctcgcctatc tcatctaaca gcaccgaacc accgttcgcc tgctcaaaga accctttttt
     3241 accttccgga gcatgaccaa acagttcact ctcgaccgca tcttccggta tagacgcgca
     3301 gttcagcgcc aggtaaggtt tgcccgctcg tgggcttgcc tgatggcagg cgtaggcaaa
     3361 gagatcttta cctgtaccgg tgtcacccgt aatcagcaac ggcgcgctta gcatcgccag
     3421 tttctgcgcc tgttcgacaa catgcttcat tttcgggctg acggcgacaa tttgactgaa
     3481 ggcactgacg tcctgtgcag cgacattctg caactgacgg cccatacgaa tcgtcgagcg
     3541 caacatcacc accgcaccgg tcagaacatg ttgatcattt tcatcctgaa gataaacagg
     3601 cgtaatctcc atcaggaaat tctgcccatt aataacaaca tgctcgttat gcgagtcttg
     3661 cggttcgctt tccagccagc gtaaaaaatt aaagccgtta attaattgcg cggcggtatg
     3721 gttgcgcagg cgatccaatt tttgcccaaa aagctgacag ctcgccgggt tcgccatatc
     3781 cactttgctt ttcatatcaa cagaaagcac aggctcaggc aacgcctcca gtaacgcgct
     3841 caacgccaga tgctcacgtt cggaaggcat ccacggaaca gtgcgcacat cggtaacacc
     3901 tgcaatacgg cgtatttcgg ccatcagact gctgaaactt tcaaattcca gttcggcaaa
     3961 attgaggtag attcgcccaa tggggtcaat ctcaataccg cgtaaatcaa tgcctctcag
     4021 caccagtaga tcgagtaatt cgcgggtcag accaagtcgg tcttcacaaa agacttccag
     4081 acgcatggga accttcacct gaaaaaagaa caattaatat gatgatatga cactatttgg
     4141 atagcaggaa ggtttctgtc aacaatgatt gacacaaaga cggaaaaacg ggcacttcgg
     4201 cgtaaagata tccggtcagc acccgatatt gcattatttt tcgctgggcg ttttgccctt
     4261 ttgcagcgtc tctttcacct gaccgataag ctgacgacgg aaatccccga ggcgaggttt
     4321 gtcatcttca atccacggca gcgggcggca aagttccata gctttaatcc ccagtcgtgc
     4381 cgtcagaagt ccagcaccaa tcccctgagc tgcccgggta gacaaacgcg cagcgagatc
     4441 ttgcgacatc cagtccatcc ccacttcgcg caccagttcg ctggctccgg cgaaagcgat
     4501 attcagcaat accagcttaa acaggcgcaa acggctgtaa tatcccagtt caatgccata
     4561 cagcgtggcg atgcgattga tcaaacgcag attgcgccag gcgataaaag ccatatccac
     4621 cagcgccagt gggctgaccg caatcatcaa tgttgattcc gctgccgaac ggctgatttc
     4681 gcgccgcgcc tgggcatcta aaactggctg gaccaaatgc gcatacaaac tgaccacttc
     4741 acggtcgttt tgcgtttcat ggattgaggc ataccagcgt tgcagcgccg gatgtgactg
     4801 atcgatgccc gcctgctgtg ccagtttttc gcaaaatgcg cggcctttac ccgtgccgtg
     4861 actatgcaat aaatcacgcg cttcgtcgcg ttcatgggcg cgctgtcgca agcgccataa
     4921 gcgccgccac tctgttacca cagaacctac gccagcgccg ataatcaatg ccccagcggc
     4981 acatccaccc agcgccaccc agtcctgggt ttgccaggcg ttcattgtcc actgtacacc
     5041 ctgcccgaca acgcttgcgc caaacagagc caacccgccc atcaccattt tgcgccacag
     5101 gctgcgtttc ggacgtaacg ctgcgtccat caccgcttca acttgcccct cttcttcctg
     5161 cgcttcgtcg agcgtggccg gggcaaaatt ttgcgcctga ttttcgtcaa aggtctgctg
     5221 cgccctgaat tttggattct gatcgacctc cagtggaccg tcgaaatcaa tacgtggttt
     5281 taacggttcg gtcatcgcaa tttatctcct attaaaaatt ccagcgcagc atcaaggcga
     5341 atatgcggta gcggtttgtc gacatccatc acctgcggac gaaacgcctc aaactggaag
     5401 ccttgcttat cccagaacgc ctgaccaggc aaacgtgcgg ggacttcgcc aggataaaca
     5461 gtgagcggtg cgccatcact aagtcgatta ccgcgcagcg ccgggatttt ctcaccgtta
     5521 acatcaataa tgccgctggt ggtcgcctga actgacgcca gccccaggca gtccatgctg
     5581 atcccttcga acgctgcatt ttgccaggca tcctgaatca gttgttgcag taatgaaacc
     5641 atattagcgt gctgatcgat ggtcacatgg tccgctttag tggcagcaaa caatagctta
     5701 tcgataaccg gcgaaaacaa acgccggaac agggtacgct gcccgtagtg gaaactttgc
     5761 atcagctgcg tcagcgccag acgcatatca ttaaatgcct gtggtccact gttgagcggt
     5821 tgcaggcagt ccaccagcac aatctggcgg tcaaaacgca ggaagtgatt tttatagaat
     5881 cctttcacca ccttctcgca gtaataatta aaacgctcgc gcaacattcc ggcgttggtg
     5941 tgtttatcgg cctgcgccag tttagactcg ccccaggcat cgacatctgg ccatgggaag
     6001 aattgcagcg cgggcgcacc tgccatatct cccggcaaga caaagcgccc aggctgaata
     6061 aagtgcagcc cctgctgttt acagtggtgg agataatcgg tccacgcggc ggcaatgtct
     6121 gccagccggt tttcgtcggc aggcgctagc gggtccagcc cttcgcacat cattcgccat
     6181 ttcgccgacc attcgccgcg ctgaccattg agtaagcccg tcatctggcg cgaccagctt
     6241 aaatagtcct gcgccagcat cggcaggtcg agcaaccatt cgccggggta atccacaatc
     6301 tccaaataca gcgtggaggt atccttaaag tggcgcagca gcgaatcgtt cgatttatag
     6361 cgtagcgcca ggcgaatttc gctgacgccg cgcgttggcg tcggccaggc gggaggatcg
     6421 ccatataact gcgccagtcc ttcatcatag gtgaagcgcg gaatgccaaa gtcacgctgg
     6481 ggaatgcgtt ttacgcccag caggcgttct tcacgcaccg cacttaatag cggcaaacgt
     6541 gctccggcat gaatattgag caactgatta accatcgcgg tgataaacgc tgttttgccg
     6601 ctgcggctaa gtccggttac agcgaggcgc agatgtctgt cgacaccccg attcaccagc
     6661 gcattaagtt catttttaag tcgcttcatt gctgtcctgt gctgctctga agaccagaaa
     6721 aaaatcattc gtaaggatat gaatgtgggg gccaggccat tgattatcaa ctgcccgtgt
     6781 tgtcatcgac gggcgaactc cataagatgt gcaacattca cgttcggacg gagttcctga
     6841 cctatgtccc ctactattta tgatattgcc agggttgcag gcgtatcaaa atccaccgta
     6901 tcacgcgtgc tgaataagca aaccaatatc tccccggaag cgcgcgaaaa agtgttacgg
     6961 gccattgaag aattacagta tcaaccaaac aagctggcac gcgcgctgac ctcttcgggt
     7021 tttgatgcca ttatggtgat ttctacccgt tcgaccaaaa ctacggcggg taatccgttt
     7081 ttctcggaag ttttacatgc catcaccgcc aaagctgaag aagaaggttt cgacgtgata
     7141 ttgcagacgt cgcacaacct ggcagaagac ttacaaaaat gcgaaagcaa aattaagcag
     7201 aaaatgatta aaggcattat tatgctaagt tcgccagcgg atgagtcatt ttttgcccaa
     7261 ctcgataaat atgatattcc ggtggtggta attggcaaag ttgaaggtca atatgcccat
     7321 gtttattctg tcgataccga taattttggc gacagcattg cgttgaccga tgcgttaatt
     7381 gaaagtggga atcaaaatat tgcctgcctg catgctccgc ttgatgtcca tgtctcggtt
     7441 gaccgcgtta atggctataa gcaaagtctg gctgcgcata atattgcagt gcgtgatgaa
     7501 tggattgttg acggcggtta tacccatgaa acagccttgc aagccgcacg gcaattatta
     7561 agccagtcgc cgttgccaga agccgtattt gccactgaca gcctgaaatt aatgagcatt
     7621 tatcgtgcgg cagcagagaa aaatattgct attccgcagc agttagcggt ggtgggctat
     7681 agcaatgaaa cgctgtcatt tattttaacg cctgcaccgg gcggcatcga tgttccgacg
     7741 caggagttag ggcaacaaag ctgcgagtta ttattccgct taattgccgg aaaaccgtca
     7801 ccacaaaata ttaccgttgc cacgcatatg tcgttgaaat aaatgtatgc cggatttggc
     7861 gctcgcgccg catccggcaa tttaacccat tatcagaacg agtaatttac gccgacacct
     7921 gcataatgga atttatcgct gtcgccttcg tcgtgatcct gccactcaaa cgcgtattcc
     7981 agcgaaacgg aaagaccatt ctggaaatca taaccgtaaa ataaccccac acggttaaaa
     8041 tcatggcctt cacgttcaat atcatcctgc cagtcccagt tactccagcg atccagccca
     8101 atgcgcgtat acggcgtcac cgagtggttg ccgagcgtca gcggcaaata ggcgcgaatt
     8161 tcttgcgtgg aaaactcacc gttattgcgg ctatcatcca tattgaaacc gcgctcgaga
     8221 taatagttca ctcgcaaggc aaccgtttcg ttgaaggtat attgcagacc cgtttccgtt
     8281 tcgacacggg tatcagcata accggtcgta ttcagatcgt tggcaaattt atacatcgac
     8341 agccaaccgt tgaaacgtaa atcgtcagtc agtttcacat cccagtctgg cgcaattttc
     8401 cagcgctgca tattcgccgt gtctttaccc ggttcatcaa cgtagtgata accataatta
     8461 cggaaaccac cagtcagacc gaaactgaaa tcatcgcttt cgaggaactg ataatgtacc
     8521 tccagctccg ggcgatcaaa ccacgttcca cgtttacccg cgctataatc taccggccct
     8581 tcctgataat aggccagaga aattctccac ggcccgttgg cggcattaaa atagacagaa
     8641 ggctccgcca gcccatccat atcttcgcca taaccctcga cgttttctat ttcgtacatc
     8701 gcgccgatat taaagtgcca gtcgttcttt tcctcggcct gtgcgcaggc cattcccgca
     8761 cacatcacca gtgcggtaca gggtaataat tttttcatta ttgttccttg tattatcctg
     8821 ggggaatgcc ttccccctgt atttagcgaa ttgctatttc cgtttctgca tcaaagaaat
     8881 gacatttagt catatcaaaa tgaatagtga tattttctcc tgcatgataa tcatttaacg
     8941 caccagcacg gaccaccagt tcgtgccccc caaccgtggt gtagagcata aattcagcac
     9001 cggttaattc tgccacgcta attttggcgg aaatgttatt ttcctcttgc gcgtccggat
     9061 gaatatcttc cgggcgtatt cccatcacaa tgggcttatg caaactttcc tgtgttttca
     9121 gaaccgctaa tttctcctcg gaaatggtta atttaagcgt ttccgtaacg aatttatcgc
     9181 catcaatcgt gccgcgaata aaattcatcg ccggtgatcc tataaatccg gcaacaaaca
     9241 tattcgccgg ttggttataa accgttttcg gcgcacctac ttgctgaaca atcccatcct
     9301 tcataatcac aatccgcgtc gccatggtca tcgcttcggt ctgatcgtgg gtcacgtaga
     9361 tcatggtggt gttcagtttc tgatgcagct tgctgatctc tgcgcgcatt tgcacgcgca
     9421 gcttggcatc caggttagag agcggttcat ccattaaaaa cacgcccgct tcgcgcacaa
     9481 tcgcccgccc gagcgccact cgctgacgtt gcccgccgga aagcgccccc ggcttgcgtt
     9541 tcaggtactc acgcaggccg agaatttgcg ccgcccagtt cacccgctca tcaatcacct
     9601 cgttggcgat tttttgcatc ttcaggccaa acgccatgtt gtcgtagacc gtcatatgcg
     9661 gatacagcgc gtagttcagg aacaccatcg cgatattgcg ctctttagcc ggaacgtcat
     9721 tcattcgttt gccgtcgatc aacagatcgc cgccgctgat ctcctcaagc ccggcaatca
     9781 tgcgcagcgt agtcgattta ccgcagcctg acggaccgac aaagacaatg aactctttat
     9841 cggcaatttc caggttgaag tccttcacca catgcacctg gttatcgtag attttttgaa
     9901 tatgttgtaa cgaaagctga gccatgttga ttcctttgct atacgttttg ccagaaggcc
     9961 gataaccgcg gccaggtcag ggagtccgtt gagggcaaca ataattgcgc cccggttaag
    10021 cccgcgccga tccccaccga gcgcataccg ctggcgttaa tggcgtcaat gcccgcctgc
    10081 gcatcttcaa tgccgataca tgcctgcggc ggcacgccca gtccggcaca ggcggcgaga
    10141 aagatttccg ggtccggttt cgagttttta agttgggaag cgtccgcgca gaaggtgaaa
    10201 aactcgcgca gctccagcgc tgctaaaatc gtcggcgcat tcagggagac agaagccagc
    10261 cctataggga tctgctgtgc acggagatct gccagcaaat tgcgaatgcc gggcagcaca
    10321 gcattaaccg tcagttcgcg cagtgagtgg acgtagagca gatttttgcg atacgccagt
    10381 tgcgccctct cctgcgggtt aaagtcgccc tctttgcccc cgtgttgcag aatgcgccgc
    10441 agagactcat cgcggctgat cccttttagg gattcgttaa actgcgcatc aatgctgatg
    10501 ccaatttcag cggcaatctg ctgccacgcc ttgaaatgca gatgcgcggt atcggtgatt
    10561 acaccatcca gatcgaaaat taccccttgc agtttcattc atcctcctga tgcgtggtag
    10621 cggtcccatt gaagggcaaa ataaaatcac ccaaacagaa aacagattct tcagccacag
    10681 atataagctg cccgttcaaa cgcagtgaaa cgggcgcaga agttcgaatc gcaatacgct
    10741 gcgcgtcgag agtgacctgt aattcgcagc cctgccagaa cagagggaaa gacaactgtt
    10801 gccactgctc aggtaacgct ggattgagat gcaattcacc gttacgcacg ctcaccccgg
    10861 caaaaccctg aatcgccccc agccaaatag cgccagttgc ggctgcgtgg ataccgtcat
    10921 cacagctatg cggatcagca ccaagatcga tttcagtccc ctcgcgccag aactgataac
    10981 tttgcgccaa caggccacag cgtgcggcaa caatgccgtg gatcgcttta ctcagtgaag
    11041 agtcgtgaat ggtgcgcggt tcataaaatt gcagattggc aagacacgat gccgctgagt
    11101 actgctccgg cagcatgtaa ttgagcatca ccacatcagc ttgtttgagg atctgcatct
    11161 cgttcacttc tgcgcgtgaa taatccagca gaatggtttg cttccccgcc gccgctttgt
    11221 atttcgccag attaatcgct ggtttcgcca taaacgaatc atcctgcggc aaaacgccgt
    11281 caggctgaat ttctggcagc cgcagttctt tgaggaatat ttcggcacga tgaataaacg
    11341 cgtcgtcgct acaaccaaac ttacgagcga tattcagcgc ctgctggacg ttgtaatacg
    11401 ccatatagct ggtgaaggcg ttattattga catgttcggt atattcgtct ggcccaataa
    11461 cattgtgaat ttccagacga tcgtttacct gtacagcgcg gctaatccag aattttgcgg
    11521 ttcccagaag taacgccatg ccttcgtggg caatgaaact ttcatccccc gttgcctgcc
    11581 agtattgaat aaccgcccag gcgatatcag ccaccaaatg gtgttcagcc tgcgccgagg
    11641 ccactttctg gcgcagtccg gtgcggatgt taatggcggc aaattccggc gtctcttctt
    11701 cgccgctgcg cgcgctttcc cacggaaaca gagcgccctg ccagccgttg cgtcgcgctt
    11761 tctcctgcgc gccaggcaag ttgtgccagc gataacgcag taaacttcgg gcaaccgtcg
    11821 gatcgctaaa cagatggaac ggtagcaaaa atacttctgt atcccagaaa acgtggcctt
    11881 tgtagccttc gccggttaaa cctttcgccg caatgctgct gcgctcatcg tgggcaggcg
    11941 tcatgatgcg caggtgataa agcgcataat ctaacgcttg ctgatcgtgc gcatcgccgc
    12001 cgtttaccgt gatacgacgt ttctgccacc attgacgcca gttttctgtt gatgctgcaa
    12061 gaagttggtc gtaactctgc tgcgcgcaca tttcaagctg acgaagcgac gcactgcccc
    12121 attcgtctaa agcggcttgt ctgtcgtccc gccagtcgat ccacacccgt ttttgcagcg
    12181 tcagcgtctc acctgcgtga agctgcgcgc aggtatgttg cagtaaacgg cgctctttgg
    12241 cggtatagca ttgctgcaca tcaccgctca ccttacagca acagctgatg gccacatcac
    12301 tgcgcccatc ctgggtggtg tagatcccct gcatcagatg ctgaccaaat acccgcacct
    12361 gggtttcgtc gagatgttgg cgaccgtggt tggtttgcgt ggcgtcaatg cctgttgaaa
    12421 tcagcactaa cgcgtcggcg tccagtggcg taatagtgat ttccagcgca atgagcggca
    12481 gttggtctgc cgaaacaaag cgacggctgg tgatggtgta acctgcgccg ttgctggtac
    12541 accagacaac gttgcggcgt aattcgccac tggcaaagtt aagctcacgc tgccatgctt
    12601 cgcgggataa cgagaaaatc tcaccattta tggcaatctc catccccacg acatcaggca
    12661 ggttcaccag ttcgttgatt tcacctttac ccgcccgatg ataaagccct gccagataca
    12721 tgccgcgcgt ctggcgcgtg taatcttctt catggctggc gcgaagccca agatagccgt
    12781 tcccctgcgc catcagcgaa gcatacttgt tcagggtatg ctggctgaaa tggggttccg
    12841 ttaacgttac tggcctgatc ataattccac acgcgtccct gtttctgcgg attgataaag
    12901 cgccgccacc agttgctgga tgatgtatcc ctgctcggcg tcggctatca tcacgggctt
    12961 accttgtacg tggttgataa aggcttccat actgcgcaga tgacggttgt cgtctgccat
    13021 ttcccgttgc atcagcgtca ttaactcacc gttgttatcg gtgtagatat gtgctggaaa
    13081 cagcgtcgcc ccggctttat caccacaaaa gctgacgttc ataatcgact gttcgcggat
    13141 gttgagtgca aatgaggtct caagccacag aatgccgccg ttatgaaatt caatggtgcc
    13201 aaacagcgaa tcttcgacgc tgtaggttgc cgggtcccac tcgccaaatt gaccgcagct
    13261 cttttgcgtg ccgatctttt gaaagctatg cgcagtcacg cttttcaccg ctggaaaacc
    13321 cagcacatac atcgccgcat ccagcatatg aatgccgatg tcgatcagcg ggcctccgcc
    13381 ctgcaattct ttattagtaa agacacccca gccgggaaca ccgcaacgac gcagggcgcg
    13441 ggcggtggta acgtaaatct ctcccaaaac gccgttggtc acctgttcac gcagctgttg
    13501 cgtatcgagt gcaaaacgat ggtgaaagtc gtaggccagc accttgccct gtttgcgcgc
    13561 ggtatcgcac atttcccgcg cctgttctgc cgtcatggcg ggcggttttt cgcacatcac
    13621 atggcagccc gcttccagcg ccatcagggt atgttcgtaa tgaaaacggt taggtgagca
    13681 gacgctaacc acatcaggtt tcaccgccag cagcatggcc tgcggatcgt cccacacgga
    13741 tgcattcccg tatttttctg ccagcgcctg cgcctgggaa aggcggctgt cacagacagc
    13801 caccagttcc agatcgttgc gggtgcagta gtacgaagca tgaactttat ccgccacctg
    13861 gcccgcgcct attatcgcga cccgcagcgg agagcttgtc attgcacttt tcacaaagac
    13921 ctcttagcag gtacgcaacc aggccaaaga atcacggtac gcctgggctg gatcttccgc
    13981 ccggatgcgc ccttcgtaca ccacatagcc ctgaaagtta tccgcgcgca gctgttctaa
    14041 cagcgcgtgg aaatccaggg tgccgctgcc cggctggtag cgatggttat cggcaatatg
    14101 cacatgaccg agcaggtcgc ggttgtcatg cagcgcctgc gccaggttat cttcttcgat
    14161 attcatgtga tagaaatcgc cgataatctg tacatgttta agatcgtttt cgacgatata
    14221 acggcgggca tcggcgagag tgttgatcat gtgatcctga taacggttta acggttcgag
    14281 gtacaccacg gttccggtac gtgcggccac cttttccagt acgcgcaggg aatcactcac
    14341 cattttgcgg tcgccgtcca ggctacgcgg cgaggtcatc ggcggtaagc ggaaggtaaa
    14401 catgccccat gcagccggaa cgacgatacc tttaccgccc acttctgcca gagcttcaag
    14461 aatgcgttcg atctgcttta agccattaag tcgacgctct tcgataaagt cgccaatcca
    14521 accgtcatag ccaccgcagg cggtgatcac cggtaaaccg gtttctttga ttgctgcttt
    14581 gacttcttcg agattgttga ccagcagttt gccgtcgatc tcaaaaccat cgaaacccat
    14641 ctctttgata taacgaaatt tctccagaat gttttccgga aagaacgcct gattctgtgt
    14701 gccgattttc atttttttca ttcctttgaa ttaaaacgta acgcccattt tgatgctctg
    14761 ttccggatgc tggtcgacat actgcatgta actttccggg ctattggcaa aggtcaccac
    14821 cgggtcgatt aaatcttcac aattgagata accgttcatc agcagttccc aacaggtttc
    14881 ttcgatacgt ttgcggctcc agcgcggata atccgggttc ggctcgctgc acgcacggga
    14941 gaaaacaatt ttggcgttat tgaaatgggc ttcgcgtccg aggttaaaac cttcggcaaa
    15001 cggtttggca aacgcgacat acgagatggt gccgccatat gccagacctc gtagcgccga
    15061 ttgcagcgcg tcggcatagc cgctggtttc gataatcaca tcggcaccct gcttgccggt
    15121 tagcgttttg atctctttgc ctacgtcact gccaatgggg ttaaggcaga aatccgcgcc
    15181 atggcgacgg gcaatctcac agcgatgggc gataggatcg acgccaatca ccaccgacgc
    15241 gccagcacgt ttagccagtt gaatggcaat ttgaccgatc gcgccaagcc ctaccaccac
    15301 cacaaaatcg cccacgcgca cattggcatc gcgcacgccg ctcatggcaa actgcgccgg
    15361 gtcgtagcag acagcgtttt tccacgaact gccttgcggc attttgcgca gcttgtagtt
    15421 attcactgcg ttaatgatga ccgtctcgga gagcgggccg tagccgcata cgctgtcgcc
    15481 caccgcgtag tcggtaacgt cgctgccgca ctcgataatg tcgccaacca ccatgttgcc
    15541 aagctggaat ttgccaaact caatgccgcg cggcgcatct gccggacgcg gggtgaacat
    15601 ctgccattcg ccgttaaagt cttcatcgat aaacggactg gcagcgcgga aatcgaccac
    15661 ttccgtcccg tgcttcggtg cgccgaagcg cacgcggatc ttcacttcat tggctaaaat
    15721 cgcccgatct tcatactcaa ccagcgcagc tacgcgcggt gctgtggcta ctaacttttt
    15781 catgtaaaac tccttgtaaa gaaactggcc gctttagccc ttcacgccac cggcggtcaa
    15841 accactctta ataaaacgct ccgaaagcgc atacataatc accaccggca atgcggtcac
    15901 cagcgaggcc gccatcatcc gtccccagat gtaatcaggc gtactgaaca gcgcgttcag
    15961 gccgactggt aaggtgaaat tgctggcgct ggagaggaaa atcgacgcaa acagatagtc
    16021 gttccatgcc accataaagc agtagacgaa tacggatatc agcccggaca tcgccagggg
    16081 cacggtgata cggaagatga tttgcagacg gttgaggcca tccatcatcg ccgcttcttc
    16141 gatctcatca gggatggtgt cgaagtagct tttcagcata aacacggcgg tgggcagggt
    16201 ttgcgttacc atggtaatga tcagcgccat ctcggtgtcg tatatgccaa gcgcggtgat
    16261 gattttgaac agcggcacca ccagcaaaat gccggagaac atatacaccg tgtaaaagct
    16321 ggcgttgatg gtcatccgcc ctttaaagcg caggcgggaa agcgcgtaag ccccgagaat
    16381 gccgagaaat accgccacca cggatgaaac caccgacacc accatgctgt tacggaagta
    16441 gtcgacaaac gggaaaatca tcgggttaaa aatgtcgaca taatgctcca gcgtccactg
    16501 ttgcggcagc agcgtaggat gcagcgagat cgcctctttt gcgctcttga acgaggtcat
    16561 cagcatcaca aaaaacggaa acagcgtgat gatgagaaac agcgccagcc cgcagtaaaa
    16621 accgatgcga ctgagtgtgc gtttatttgt tgccattgag gttcacccgt tttctggtca
    16681 gcagaatgac agcgaaaata atgatgaaga gcaccaccga gatcgccgcc gctttcccta
    16741 agtcattaaa ggcaaaggcg gttttgtaga gatagacacc gagaatatcg accttggtcg
    16801 tcagcagata aacatccgcg aacatgtaga acatccagat ggtgcgcagg gtcaccaccg
    16861 tcgccaggac cggcataatc gcgggcagcg tgacgatacg aaaccgttgc caggcattag
    16921 cgccatccat ctctgccgct tcgtacagcg atttgtcgat ggtctgcaaa atcgccagaa
    16981 acgaaataaa ggcatagggg aagtagcgcc agatggcgaa cagcaccacc agcacaaagc
    17041 tactgcccgg attatcgaac cacagcggtg cctgctcata gagatgcaga agatcgacgc
    17101 cgaggtagtt cacaatgccg taaccgttgt tgaacatgta tttccaggcg aacaccagcg
    17161 aaatagacgg cgttacgtag gagaggatca ccagcgaacg cgcggttttg cgcaggcgga
    17221 actcgcggtt gaaaaacatc gccacggcca gcccgagcac ggtgctgccc gccaccacca
    17281 gcgcggtata ccagaccgtc atccacagcg agtgccagaa gccgggatcg gagagaatac
    17341 gcacgtagtt gctcacccca acaaacgttg actcgatgtt gggattaagc ggcagacgta
    17401 aaaaactgat ttcgatattc gacaccatcg gccacgccac cagaccgccc agcagtaata
    17461 agctgggcgc gagaagcaac agcgcaaagg gcatatcgga acgacctgaa aaaagcctgt
    17521 tcattgcgga ttcctgtcct gggtttagcg ctgttcaacc aactcatcca gctttttctg
    17581 gctcgtttgc agcgtgccag ggaggtcggc tttacccacg gtgacgttat gcaccatgga
    17641 gctcaccacg ccagaacccg tcacatcgcc catgcgggta aaatttttat cccctactgc
    17701 gccaaagacc tgaatatttg gcagttcgct gattaactga tttggcagct cacccagcgc
    17761 cttaataacg tcgttgtctt tccaggtggc ggtagtgaca accgctttgt tcaccggcag
    17821 cgccgcgcct ggcgacatca tcacccagtc ggcaatgttg tctgcctgct ccataaaggt
    17881 gacaaatttt tcagccgctt ccgtctcttc ggccttttgc ccgacggtaa tggtcagcga
    17941 ggtcaacatg ccgtagaccg cagagttttt ctcggtgggt acaacgaaac caacgttttt
    18001 cggatcgcct tctttaatca cagccggaag gatataggtg gagtaaatcg ccatcggcgc
    18061 ggtgccgttc ataaaagcgt ctttcacttc catgatgtcg ttagaacctg gcatggtgtt
    18121 ggcagcaagg ttgcggtaat aggtcagcgc ctgcatcatc tctggtgtat caagggtgat
    18181 tttgccttcg gcgttaaaga cgttagcctg attggataac gcaaactggg agaaagactg
    18241 ttccgtcaac acgctttctg ctgtaggcag cgcaataccg tattttttat tcgccgggtc
    18301 attcagtttc tgtgcaacgt ccagcaattg ctgccagttt tttggctcct ccagcccagc
    18361 ttttgccagc acatctttgc gataccagat cccgccaatc catgcgctga caggaacacc
    18421 ggtccaggcg ctgccatctt cggtgcgcac aatccgcagt acgccatcgt agaacgcgcc
    18481 ttcaccgacg ttgctgatga ccgtggcaac cgctttacga tcgataagct gctctttgtc
    18541 catcactttg gcgtagtcat ggctggtttc gatcacttcc ggcagcgaac cgctacgtga
    18601 aagggtaatg actttggtgt tataggcgtc ttcttccacg ggcacttgtt ttacggtgat
    18661 acctgggttt tctttttcaa aacgggcaat caatttgctg atgacggcct ggcgctcctg
    18721 ctccaccgaa gaatgcataa attcgatgga tacattattt ttattttctt ctttacagcc
    18781 tgaaatcagg gcgcaggaaa ccagtgctga taacagcacg atttttgatt taatcatgaa
    18841 atgttccttt ttatttaatc cacataacct gccagggatg cagtgttatt tcattaccca
    18901 atatatcaac ttcactaatc agatcccgac cgtgcaggtc agtaatattt atatgctgaa
    18961 tattttcact gacattaaac aatcccgtca ggcaatcgcc atccgcattg cttctttgaa
    19021 ttcgcattac aaatgaatta atcgcatcaa tggtgaaatt attatccgga tgaaactctt
    19081 tatgactgcg acgaagtgta a
//
LOCUS       DAZUCC010000088        19075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZUCC010000088 DAZUCC010000000
VERSION     DAZUCC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.088"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(81..1364)
                     /gene="gltA"
                     /locus_tag="ACHY09_002623"
     CDS             complement(81..1364)
                     /gene="gltA"
                     /locus_tag="ACHY09_002623"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFS3885578.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1570..1677)
                     /locus_tag="ACHY09_002624"
     CDS             complement(1570..1677)
                     /locus_tag="ACHY09_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885579.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2058..2462
                     /gene="sdhC"
                     /locus_tag="ACHY09_002625"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2058..2462
                     /gene="sdhC"
                     /locus_tag="ACHY09_002625"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFS3885580.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2456..2803
                     /gene="sdhD"
                     /locus_tag="ACHY09_002626"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2456..2803
                     /gene="sdhD"
                     /locus_tag="ACHY09_002626"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFS3885581.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2803..4569
                     /gene="sdhA"
                     /locus_tag="ACHY09_002627"
     CDS             2803..4569
                     /gene="sdhA"
                     /locus_tag="ACHY09_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFS3885582.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASASDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4585..5301
                     /gene="sdhB"
                     /locus_tag="ACHY09_002628"
     CDS             4585..5301
                     /gene="sdhB"
                     /locus_tag="ACHY09_002628"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFS3885583.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5602..8403
                     /gene="sucA"
                     /locus_tag="ACHY09_002629"
     CDS             5602..8403
                     /gene="sucA"
                     /locus_tag="ACHY09_002629"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFS3885584.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8418..9635
                     /gene="odhB"
                     /locus_tag="ACHY09_002630"
     CDS             8418..9635
                     /gene="odhB"
                     /locus_tag="ACHY09_002630"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFS3885585.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPVKESAP
                     AAPAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9729..10895
                     /gene="sucC"
                     /locus_tag="ACHY09_002631"
     CDS             9729..10895
                     /gene="sucC"
                     /locus_tag="ACHY09_002631"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFS3885586.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVSVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10895..11764
                     /gene="sucD"
                     /locus_tag="ACHY09_002632"
     CDS             10895..11764
                     /gene="sucD"
                     /locus_tag="ACHY09_002632"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFS3885587.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11868..12590)
                     /gene="mngR"
                     /locus_tag="ACHY09_002633"
     CDS             complement(11868..12590)
                     /gene="mngR"
                     /locus_tag="ACHY09_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFS3885588.1"
                     /translation="MGRKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLTDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12760..14676
                     /gene="mngA"
                     /locus_tag="ACHY09_002634"
     CDS             12760..14676
                     /gene="mngA"
                     /locus_tag="ACHY09_002634"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFS3885589.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALNLKRSDETRTVQQDT
                     QPVKSLKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14694..17327
                     /gene="mngB"
                     /locus_tag="ACHY09_002635"
     CDS             14694..17327
                     /gene="mngB"
                     /locus_tag="ACHY09_002635"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFS3885590.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLTFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKLWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEITHEAWQSRAVIR
                     YDMAVPRNLHERSARQSTGRVGVELAVTLSDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGLAVF
                     SEGLREYEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGSFSC
                     RLSLFSYTGTPSTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVVLRLFNPAESTTCEATVAFSREVISCTQTMMDESISTAERD
                     NLKELGAFLPGQSRTFSYRLG"
     gene            complement(17472..18869)
                     /locus_tag="ACHY09_002636"
     CDS             complement(17472..18869)
                     /locus_tag="ACHY09_002636"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFS3885591.1"
                     /translation="MHHLSRLDKAFLRKGIQLSRANNIVSLSRPSGASARVLLPDELP
                     LEEKAVTQLLDFAEVKLPDHPGYVRQTCATPDFHPGNGVPVGAVVASTPDLVIPAAIG
                     TDISCGMRLLTTGLTLEQAEAHKDVIAQRLTQVLLQNERDVPLSAQAFTALFDGGPAD
                     CVSTLPSEGLWIRTNIPRMVQEIDRCIGLNEHNSHSRYAPPALQATDRALLRDPCLGT
                     PGSGNHFIELQVVDSVVDRHVAWQQGLKTGDIVVMIHSGSRDVGFYVGRHWMDRARAE
                     WPTGHKHPQSGLYGLAGGLAEEYLQAIGMAARYAWLNRVTIAEMVRVCLANLFHQDNS
                     HLVVDVPHNIILREHEMNIHRKGATPARVGNLALIPGSMGDYSWLAVGCGNPEWLWSC
                     SHGAGRSQRRQAMRSRATEESTLPWQCVTLREERRFEEAPAAYKDIGPVIEAQQEAGL
                     IQPAVRFRPWLTFKG"
BASE COUNT         4601 a         4776 c         5188 g         4510 t
ORIGIN      
        1 tttattatgt tcagccatat aaaaaagaac ccgctctatt aaaggcgggt ccggaaagta
       61 aacggcttag caatcaacca ttaacgcttg atgtcgcttt taaagtcgcg tttttcatat
      121 cctgtataca gttgacgcgg acgggcaatc ttcataccgt cactgtgcat ttcgctccag
      181 tgggcgatcc agccaacggt gcgtgccata gcgaagatca cggtgaacat agaagacgga
      241 atacccatcg ctttcaggat gataccagag tagaaatcga cgttcggata gagtttcttc
      301 tcgataaagt acgggtcgtt cagcgcgatg ttttccagct ccatagccac ttccagcagg
      361 tcatctttgg tacccagctc tttcagcact tcatggcagg tttcacgcat tacggtggcg
      421 cgcgggtcgt aatttttgta cacgcggtga ccgaagccca tcaggcggaa agaatcattt
      481 ttatctttcg cacgacgaac aaattccgga atgtgtttaa cggagctgat ttcttccagc
      541 attttcagcg ccgcttcgtt agcaccaccg tgcgcaggtc cccacagtga agcaatacct
      601 gctgcgatac aggcaaacgg gttcgccccc gaagagccag cggtacgcac ggtggaggtg
      661 gaggcgttct gttcatggtc agcgtgcagg atcagaatac ggtccatagc acgttccaga
      721 atcggattaa cttcatacgg ttcgcacggc gtagagaaca tcatattcag gaagttaccg
      781 gcgtaggaga gatcgttgcg cgggtaaaca aatggctgac caatggaata cttgtaacac
      841 atcgcggcca tggtcggcat tttcgacagc aggcggaacg cggcaatttc acggtgacga
      901 ggattgttaa catccagcga gtcgtgatag aacgccgcca gcgcgccggt aataccacac
      961 atgactgcca ttggatgcga gtcgcgacgg aaagcatgga acagacgggt aatctgctcg
     1021 tggatcatgg tatgacgggt caccgtagtt ttaaattcgt catactgttc ctgagtcggt
     1081 ttttcaccat tcagcaggat gtaacaaact tccaggtagt tagaatcggt cgccagctga
     1141 tcgatcggga aaccgcggtg cagcaaaata ccttcatcac catcaataaa agtaatttta
     1201 gattcgcagg atgcggttga agtgaagcct gggtcaaagg tgaatacacc ttttgaaccg
     1261 agagtacgga tatcaataac atcttgaccc agcgtgcctt tcagcacatc cagttcaaca
     1321 gctgtatccc cgttgagggt gagttttgct tttgtatcag ccatttaagg tctccttagc
     1381 gccttattgc gtaagactgc cggaacttaa atttgccttc gcacatcaac ctggctttac
     1441 ccgtttgtta tttggctcgc cgctctgtga aagaggggaa aacctgggta cagagctctg
     1501 ggcgcttgca ggtaaaggat ccattgatga cgaataaatg gcgaatcaag tacttagcaa
     1561 tccgaattat taaacttgtc taccactaat aactgtcccg aatgaattgg tcaatacttc
     1621 cacactgtta cataagttaa tcttaggtga aataccgact tcataacttt tacgcattat
     1681 atgcttttcc tggtaatgtt tgtaacaact ttgttgaatg attgtcaaat tagatgatta
     1741 aaaattaaat aaatgttgtt atcgtgacct ggatcactgt tcaggataaa acccgacaaa
     1801 ctatatgtag gttaattgta atgattttgt gaacggccta tactgccgcc aggtctccgg
     1861 aacaccctgc aatcccgagc cacccagcgt tgtaacgtgt cgttttcgca tctggaagca
     1921 gtgttttgca tgacgcgcag ttatagaaag gacgctgtct gacccgcaag cagaccggag
     1981 gaaggaaatc ccgacgtctc caggtaacag aaagttaacc tctgtgcccg tagtccccag
     2041 ggaataataa gaacagcatg tgggcgttat tcatgataag aaatgtgaaa aaacaaagac
     2101 ctgttaatct ggacctacag accatccggt tccccatcac ggcgatagcg tccattctcc
     2161 atcgcgtttc cggtgtgatc acctttgttg cagtgggcat cctgctgtgg cttctgggta
     2221 ccagcctctc ttcccctgaa ggtttcgagc aagcttccgc gattatgggc agcttcttcg
     2281 tcaaatttat catgtggggc atccttaccg ctctggcgta tcacgtcgtc gtaggtattc
     2341 gccacatgat gatggatttt ggctatctgg aagaaacatt cgaagcgggt aaacgctccg
     2401 ccaaaatatc ctttgttatt actgtcgtgc tttcacttct cgcaggagtc ctcgtatggt
     2461 aagcaacgcc tccgcattag gacgcaatgg cgtacatgat ttcatcctcg ttcgcgctac
     2521 cgctatcgtc ctgacgctct acatcattta tatggtcggt tttttcgcta ccagtggcga
     2581 gctgacatat gaagtctgga tcggtttctt cgcctctgcg ttcaccaaag tgttcaccct
     2641 gctggcgctg ttttctatct tgatccatgc ctggatcggc atgtggcagg tgttgaccga
     2701 ctacgttaaa ccgctggcct tgcgcctgat gctgcaactg gtgattgtcg ttgctctggt
     2761 ggtttacgtg atttatggat tcgttgtggt gtggggtgtg tgatgaaatt gccagtcaga
     2821 gaatttgatg cagttgtaat tggtgccggt ggcgcaggta tgcgcgcggc gctgcaaatt
     2881 tcccagagcg gtcagacctg tgcgctgctc tctaaagtct tcccgacccg ttcccatacc
     2941 gtttctgcgc aaggcggcat taccgttgcg ctgggtaata cccatgaaga taactgggaa
     3001 tggcatatgt acgacaccgt aaaaggttcg gactatatcg gtgaccagga cgcgattgaa
     3061 tatatgtgta aaaccgggcc ggaagcgatt ctggaactgg aacatatggg cctgccgttc
     3121 tcgcgtcttg atgatggtcg tatctatcaa cgtccgtttg gcggtcagtc gaaaaacttc
     3181 ggcggcgagc aggcggcacg tactgcggcg gcagctgacc gtaccggtca cgcactgttg
     3241 cacacccttt atcaacagaa cctgaaaaac cacaccacca ttttctccga gtggtatgcg
     3301 ctggatctgg tgaaaaacca ggatggcgcg gtggtcggtt gtaccgcact gtgcatcgaa
     3361 actggtgaag tggtttactt caaagctcgc gctaccgtgc tggcgactgg cggggcaggg
     3421 cgtatttatc agtccactac caacgcccac attaacactg gcgacggcgt cggcatggct
     3481 atccgtgcag gcgtaccagt gcaggatatg gaaatgtggc agttccaccc gactggtatt
     3541 gccggtgcgg gcgtactggt caccgaaggg tgccgtggtg aaggcggtta tctgctgaac
     3601 aaacatggcg aacgctttat ggagcgttat gcaccgaacg ccaaagacct ggcgggccgt
     3661 gacgtggtgg cgcgttccat catgattgaa atccgtgaag gtcgcggctg tgatggtccg
     3721 tgggggccac acgcgaaact gaaacttgac catctgggta aagaagttct tgaatcccgt
     3781 ctgccgggta tccttgaact ctcccgcacc ttcgctcacg ttgatccggt gaaagagccg
     3841 attccggtta tcccaacctg tcactacatg atgggcggta ttccgaccaa agtgaccggt
     3901 caggcgctga cggtgaacga gaaaggcgaa gatgtggttg ttccggggct atttgccgtt
     3961 ggtgaaatcg cttgtgtatc ggtacatggc gctaaccgtc tgggcggcaa ctcgctgctg
     4021 gacctggtcg tatttggtcg tgcggcaggt ctgcatctgc aagagtctat cgccgagcag
     4081 ggcgcactgc gcgatgccag cgcgtctgat gtagaagcgt ctctggatcg cctgaaccgc
     4141 tggaacaata accgtaacgg tgaagatccg gtggcgatcc gtaaagcact gcaagaatgt
     4201 atgcagcata acttctcggt cttccgtgaa ggtgatgcga tggcgaaagg gcttgagcag
     4261 ttgaaagtta tccgcgagcg tctgaaaaat gcccgtctgg atgacacttc aagtgagttc
     4321 aatacccagc gcgttgagtg cctggaactg gataacctga tggaaacggc gtatgcaacg
     4381 gctgtttctg ccaacttccg taccgaaagc cgtggcgcac atagccgctt cgacttcccg
     4441 gatcgtgatg atgaaaactg gctgtgccac tccctgtatc tgcctgagtc ggaatccatg
     4501 acgcgccgaa gcgtcaacat ggaaccgaaa ctgcgcccgg cattcccgcc gaagattcgt
     4561 acttactaat gcggagacag gaaaatgaga ctcgagtttt caatttatcg ctataacccg
     4621 gatgttgatg atgctccgcg tatgcaggat tacaccctgg aagcggaaga aggtcgtgac
     4681 atgatgctgc tggatgcgct tatccagctg aaagagaaag atcccagcct gtcgttccgt
     4741 cgctcctgcc gtgaaggtgt gtgtggttcc gacggtctga acatgaacgg caagaatggt
     4801 ctggcctgta ttaccccgat ttcggcactc aaccagccgg gcaagaagat tgtgattcgc
     4861 ccgctgccag gtttaccggt gatccgcgat ttggtggtag acatgggaca attctatgcg
     4921 caatatgaga aaattaagcc ttacctgttg aataatggac aaaatccgcc agctcgcgag
     4981 catttacaga tgccagagca gcgcgaaaaa ctcgacgggt tgtatgaatg tattctctgc
     5041 gcatgttgtt caacctcttg tccgtctttc tggtggaatc ccgataagtt tatcggcccg
     5101 gcaggcttgt tagcggcata tcgtttcctg atcgatagcc gtgataccga gactgacagc
     5161 cgcctcgacg gtttgagcga tgcattcagt gtattccgct gtcacagcat catgaactgc
     5221 gtcagtgtat gtccgaaggg gctgaacccg acgcgcgcca tcggccatat caagtcgatg
     5281 ttgttgcaac gtaatgcgta aaccgtaggc ctgagaagac gcgcaagcgt cgcatcaggc
     5341 aaccagtgcc ggatgcggcg tgaacgcctt atccggccta caagtcatta cccgtaggcc
     5401 tgataagcgc agcgcatcag gcgtaacaaa gaaatgcagg aaatctttaa aaactgcccc
     5461 tgacactaag acagttttta aaggttcctt cgcgagccac tacgtagaca agagctcgca
     5521 agtgaacccc ggcacgcaca tcactgtgcg tggtagtatc cacggcgaag taagcataaa
     5581 aaagatgctt aagggatcac gatgcagaac agcgctttga aagcctggtt ggactcttct
     5641 tacctctctg gcgcaaacca gagctggata gaacagctct atgaagactt cttaaccgat
     5701 cctgactcgg ttgacgctaa ctggcgttcg acgttccagc agttacctgg tacgggagtc
     5761 aaaccggatc aattccactc tcaaacgcgt gaatatttcc gccgcctggc gaaagacgct
     5821 tcacgttact cttcaacgat ctccgaccct gacaccaatg tgaagcaggt taaagtcctg
     5881 cagctcatta acgcataccg cttccgtggt caccagcatg cgaatctcga tccgctggga
     5941 ctgtggcagc aagataaagt ggccgatctg gatccgtctt tccacgatct gaccgaagca
     6001 gacttccagg agaccttcaa cgtcggttca tttgccagcg gcaaagaaac catgaaactc
     6061 ggcgagctgc tggaagccct caagcaaacc tactgcggcc cgattggtgc cgagtatatg
     6121 cacattacca gcactgaaga aaaacgctgg atccaacagc gtatcgagtc tggtcgcgcg
     6181 actttcaata gcgaagagaa aaaacgcttc ttaagcgaac tgaccgccgc tgaaggcctt
     6241 gaacgttacc tcggcgcaaa attccctggc gcaaaacgct tctcgctgga aggtggtgac
     6301 gcgttaatcc cgatgcttaa agagatgatc cgccacgctg gcaacagcgg cacccgcgaa
     6361 gtggttctcg ggatggcgca ccgtggtcgt ctgaacgtgc tggtgaacgt gctgggtaaa
     6421 aaaccgcaag acttgttcga cgagtttgcc ggtaaacata aagaacacct cggcacgggc
     6481 gacgtgaaat accacatggg cttctcgtct gacttccaga ccgatggcgg cctggtgcac
     6541 ctggcgctgg cgtttaaccc gtctcacctt gagattgtca gcccggtcgt tatcggttct
     6601 gttcgtgccc gtctggacag acttgatgag ccgagcagca acaaagtgct gccaatcacc
     6661 attcatggtg acgccgcagt gaccgggcag ggcgtggttc aggaaaccct gaacatgtcg
     6721 aaagcgcgtg gttatgaagt tggcggtacg gtacgtatcg ttatcaacaa ccaggttggc
     6781 ttcaccacct ctaacccgct ggatgcccgt tcgacgccgt actgtactga tatcggcaag
     6841 atggtacagg caccgatttt ccacgttaat gcggatgatc cggaagccgt tgcctttgtg
     6901 acccgtctgg cgctcgattt ccgtaacacc tttaaacgtg atgtcttcat cgatctggtg
     6961 tgctaccgcc gtcacggcca caacgaagcc gacgagccga gcgcaaccca gccgctgatg
     7021 tatcagaaaa tcaaaaaaca tccgacgccg cgcaaaatct atgctgacaa gctggagcag
     7081 gaaaaagtcg cgacgctgga agatgccacc gagatggtta acctgtaccg cgatgcgctg
     7141 gatgctggcg attgcgttgt agcagagtgg cgtccgatga acatgcactc tttcacctgg
     7201 tcgccgtacc tcaaccacga atgggacgaa gagtacccga acaaagttga gatgaagcgc
     7261 ctgcaggaac tggctaaacg catcagcacg gtgccggaag cagttgaaat gcagtctcgc
     7321 gttgccaaga tttatggcga tcgccaggca atggcagccg gtgagaaact gttcgactgg
     7381 ggcggtgcgg aaaacctcgc ttacgccacg ctggttgatg aaggcattcc ggttcgcctg
     7441 tcgggtgaag actccggtcg cggtaccttc ttccaccgcc acgcggtgat ccacaaccag
     7501 tctaacggtt ccacttacac gccgctgcaa cacatccata acggtcaggg cgcgttccgt
     7561 gtctgggact ccgtactgtc agaagaagcc gtgctggcgt ttgaatacgg ttatgccacc
     7621 gcagaaccac gcactctgac catctgggaa gcgcagttcg gtgacttcgc caacggtgcg
     7681 caggtggtta tcgaccagtt catctcctct ggcgaacaga aatggggccg gatgtgtggc
     7741 ctggtgatgt tgctgccgca cggttacgaa gggcaggggc cggagcactc ctccgcgcgt
     7801 ctggaacgtt atctgcaact ttgcgctgag caaaacatgc aggtttgcgt accgtctacc
     7861 ccggcacagg tttaccacat gctgcgtcgt caggcgttgc gcgggatgcg tcgtccactg
     7921 gtcgtgatgt cgccgaaatc cctgctgcgt catccgctgg cggtatccag cctcgaagaa
     7981 ctggcgaacg gcaccttcct gccagccatc ggtgaaatcg acgagcttga tccgaagggc
     8041 gtgaagcgcg tagtgatgtg ttctggtaag gtttattacg acctgctgga acaacgtcgt
     8101 aagaacaatc aacacgatgt cgccattgtg cgtatcgagc aactctaccc gttcccgcat
     8161 aaagcgatgc aggaagtgtt gcagcagttt gctcacgtca aggattttgt ctggtgccag
     8221 gaagagccgc tcaaccaggg cgcatggtac tgcagccagc atcatttccg tgaagtgatt
     8281 ccgtttgggg cttctctgcg ttatgcaggc cgcccggcct ccgcctctcc ggcggtaggg
     8341 tatatgtccg ttcaccagaa acagcaacaa gatctggtta atgacgcgct gaacgtcgaa
     8401 taaataaagg atacacaatg agtagcgtag atattctggt ccctgacctg cctgaatccg
     8461 tagccgatgc caccgtcgca acctggcata aaaaacccgg cgacgcagtc gtacgtgatg
     8521 aagtgctggt agaaatcgaa actgacaaag tggtactgga agtaccggca tcagcagacg
     8581 gcattctgga tgcggttctg gaagatgaag gtacaacggt aacgtctcgt cagatccttg
     8641 gtcgcctgcg tgaaggcaac agcgcgggta aagaaaccag cgccaaatct gaagagaaag
     8701 cgtccactcc ggcgcaacgc cagcaggcgt ctctggaaga gcaaaacaac gatgcgttaa
     8761 gcccggcgat ccgtcgcctg ctggctgaac ataacctcga cgccagcgcc attaaaggca
     8821 ccggtgtggg tggtcgtctg acccgtgaag atgtggaaaa acatctggcg aaagccccgg
     8881 tgaaagagtc tgctccggca gcgcctgctc cggcggcgca accggccctg gctgcacgta
     8941 gcgaaaaacg tgtgccgatg actcgcctgc gtaagcgtgt ggcagagcgt ctgctggaag
     9001 cgaaaaactc caccgccatg ctgaccacgt tcaacgaagt caacatgaag ccgattatgg
     9061 atctgcgtaa gcagtacggt gaagcgtttg aaaaacgcca cggcatccgt ctgggcttta
     9121 tgtccttcta cgtgaaagcg gtggttgaag ccctgaaacg ttacccggaa gtgaatgcgt
     9181 ctatcgacgg tgatgatgtg gtctaccaca actatttcga cgtcagtatg gcggtttcta
     9241 cgccgcgcgg cctggtgacg ccggttctgc gtgatgtcga taccctcggc atggcagaca
     9301 tcgagaagaa aatcaaagag ctggctgtta aaggccgtga cggcaagctg acggttgaag
     9361 atctgactgg tggtaacttc accatcacca acggtggtgt gttcggttcc ctgatgtcaa
     9421 cgcctatcat caacccaccg cagagcgcaa ttctgggtat gcacgctatc aaagatcgtc
     9481 cgatggcggt gaatggtcag gttgagatcc tgccgatgat gtacctggcg ctgtcctacg
     9541 atcaccgtct gatcgatggt cgcgaatccg tgggcttcct ggtaacgatc aaagagttgc
     9601 tggaagatcc gacgcgtctg ctgctggacg tgtagtagtt taagcttcac ctgcactgta
     9661 gaccgacgtg gatagggtgt cggtctacgg tttaaaagat aacgattact gaaggatgga
     9721 cagaacacat gaacttacat gaatatcagg caaaacaact ttttgcccgc tatggcttac
     9781 cagcaccggt gggttatgcc tgtactactc cgcgcgaagc agaagaagcc gcttcaaaaa
     9841 tcggtgccgg tccgtgggta gtgaaatgtc aggttcacgc tggtggccgc ggtaaagcgg
     9901 gcggtgtgaa agttgtaaac agcaaagaag acatccgtgc ttttgcagaa aactggctgg
     9961 gcaagcgtct ggtaacgtat caaacagatg ccaatggcca accggttaac cagattctgg
    10021 ttgaagcagc gaccgatatc gctaaagagc tgtatctcgg tgccgttgtt gaccgtagtt
    10081 cccgtcgtgt ggtctttatg gcctccaccg aaggcggcgt ggaaatcgaa aaagtggcgg
    10141 aagaaactcc gcacctgatc cacaaagttg cgcttgatcc gctgactggc ccgatgccgt
    10201 atcagggacg cgagctggcg ttcaaactgg gtctggaagg taaactggtt cagcagttca
    10261 ccaaaatctt catgggcctg gcgaccattt tcctggagcg cgacctggcg ctgatcgaaa
    10321 tcaacccgct ggtcattacc aaacagggcg atctgatttg cctcgacggc aaactgggcg
    10381 ctgatggcaa cgcactgttc cgccagcctg atctgcgcga aatgcgtgac cagtcgcagg
    10441 aagatccgcg tgaagcacag gctgcacagt gggaactgaa ctacgttgcg ctggacggta
    10501 acatcggttg tatggttaac ggcgcaggcc tggcgatggg caccatggac atcgttaaac
    10561 tgcacggcgg cgaaccagct aacttcctgg acgttggcgg cggcgcaacc aaagaacgtg
    10621 taaccgaagc gttcaaaatc atcctctctg acgacaaagt gaaagccgtt ctggttaaca
    10681 tcttcggcgg tatcgttcgt tgcgacctga tcgctgacgg tatcatcggc gcggtagcgg
    10741 aagtgggtgt taacgtatcg gtcgtggtac gtctggaagg taacaacgcc gaactcggcg
    10801 cgaagaaact ggctgacagc ggcctgaata ttattgcagc aaaaggtctg acggatgcag
    10861 ctcagcaggt tgttgccgca gtggagggga aataatgtcc attttaattg ataaaaacac
    10921 caaggttatc tgccagggct ttaccggtag ccaggggact ttccactctg aacaagccat
    10981 tgcatacggc actaaaatgg ttggcggcgt aaccccaggt aaaggcggca ccacccacct
    11041 cggcctgccg gtgttcaaca ccgtgcgtga agctgttgct gctactggcg ctaccgcttc
    11101 tgttatctac gtaccagcac cgttctgcaa agactccatt ctggaagcta tcgatgcagg
    11161 catcaaactg attatcacca tcactgaagg catcccgacg ctggatatgc tgaccgtgaa
    11221 agtgaagctg gatgaagcag gcgttcgtat gatcggcccg aactgcccag gcgttatcac
    11281 tccgggtgaa tgcaaaatcg gtatccagcc tggtcacatt cacaaaccgg gtaaagtggg
    11341 tatcgtttcc cgttccggta cgctgaccta tgaagcggtt aaacagacca cggattacgg
    11401 tttcggtcag tcgacctgtg ttggtatcgg cggcgatccg atccctggct ctaactttat
    11461 cgacatcctc gaaatgttcg aaaaagatcc gcagaccgaa gcgatcgtga tgatcggtga
    11521 gatcggcggt agcgctgaag aagaagcagc ggcgtacatc aaagagcacg ttaccaagcc
    11581 agttgtaggt tacatcgctg gtgtgactgc gccgaaaggc aaacgtatgg gccacgcggg
    11641 tgcgatcatt gccggtggga aagggactgc ggatgagaaa ttcgctgctc tggaagccgc
    11701 aggcgtgaaa accgttcgca gcctggcgga tatcggtgaa gcactgaaaa ctgttctgaa
    11761 ataaatatct gtaataagaa atagccctcg ccgcttcctt ctacaggaat ggcgaagggc
    11821 tgtcggtttc gacatggttg gccatcgtat gatggccttt tttatgctta tcgcgatgat
    11881 tttcgctgcg ctatcagggt aaatttatag tcatcggtat taaaagcgtt gcggctatat
    11941 tcaaacaccc ggccatcaac taaatatcca cgcgatactt tttcaagaat cggcttagtc
    12001 tggctgatat taagcagacg gctcatttct tcggttggca tcagaggaat gatttcctgt
    12061 tcgctacgat cgataaccat tttcttcact tcttcaataa agtgatattt cgaattttcc
    12121 atgacctgcc aggtgagatc cgggaacaac gcaagcggca tccaggtttc ttccagcgcc
    12181 attggctttt gcttgcgata gcgcacgcgc ttcacatgcc acacacgatc ctgcgcggtg
    12241 atttgtagct gttgctgaag aaaatcgtca gccggaatca cttcgaatat cagaacttca
    12301 ctgtgtgtat cgacgtgacg gtccgtcagt ttttcatcaa aactggttaa ctgaaaaata
    12361 tcgtaattga cccgctcttc ttttacgtaa gtcccgctgc cctgaatact ttcgaggatc
    12421 tgctgttcaa ctaactggcg caaagcctga cgcaccgtaa cccggctgac gccaaactct
    12481 gtttgtagcg ctgattcagt gggtaacgca tcgccaggtt taagctcgcc acgcgcaatt
    12541 tgctcacgaa tgcgatcagc aatctgtcgg tataagggct tccgtcccat gatttagcat
    12601 ctcattaata cgaatttagc cattatgccc gataaattca tcctgtaaat aatacaaata
    12661 caatacaaat aatttcaatc aagtgaaatt gatcacataa tggtattgtt ttatcgggca
    12721 cactggcgcg actataaaaa cgatcaagtg aggatcatga tgaacctgac gactctgacc
    12781 caccgcgatg cgttgtgtct gaatgcgcgc tttaccagcc gtgaagaggc catccacgcg
    12841 ttgactcaac gtcttgctgc tctggggaaa atttccagta ctgagcaatt tctgaaagaa
    12901 gtgtatcgcc gtgaaagcct tggcccgacc gccttaggtg aagggctggc tgtgccgcat
    12961 ggcaaaactg ctgcggtaaa agaagcggcg tttgcggtcg ccacactcag cgagccgctt
    13021 cagtgggaag gcgtggatgg cccggaagcg gttgatttag tggtgttgct ggcgattccc
    13081 cccaatgaag cgggtactac gcacatgcaa ctgctgacag cgctgaccac gcgcctggcg
    13141 gatgatgaga tacgggcgcg tatacagtcg gcgacgacgc ctgatgaatt gctctcggcg
    13201 ctggatgaca agggaggcac gcaaccctca gcttcttttt ccaacgcgcc aactatcgtc
    13261 tgcgtaacgg cctgcccggc gggtattgct cacacctata tggctgcgga gtatctggaa
    13321 aaagccggac gcaaactcgg cgtgaatgtc tacgttgaaa agcaaggtgc taacggcatt
    13381 gaagggcgtt taacggcaga gcaactcaat agtgcaaccg cctgtatttt tgcggctgaa
    13441 gtcgccatca aggagagtga gcgttttaac ggcattcccg cgctttctgt gcctgttgcc
    13501 gagccgattc gccatgcaga agcgttgatc caacaagcgc ttaacctcaa gcgtagcgat
    13561 gagacgcgta ccgtacagca agatacgcaa ccggtgaaaa gcctcaaaac ggagctaaaa
    13621 caggcactgt tgagcgggat ctcttttgca gtgccgttga ttgtcgcggg gggcacggtg
    13681 ctggcggttg cggtattact gtcgcaaatc ttcgggctac aagatttgtt taatgaagaa
    13741 aactcctggc tgtggatgta ccgcaaactg ggcggagggc tgctcgggat tttgatggta
    13801 ccggtgctgg cagcctacac cgcctattca ctggctgata aaccggcgtt agcgccaggc
    13861 tttgcggctg gacttgccgc caacatgatc ggttccgggt ttctcggtgc tgtcgttggc
    13921 ggattgatag ccggttatct gatgcgctgg gtgaaaaatc acttgcgtct tagcagtaaa
    13981 ttcaatggat tcctgacttt ttatctctac ccggtgctcg gtacgttggg agcgggcagt
    14041 ctgatgctgt ttgtggtggg ggaacctgtc gcctggatca ataactcgct taccgcctgg
    14101 ctgaacggtc tgtcaggaag taacgcgctg ttgctgggtg ccattctcgg ttttatgtgt
    14161 tcctttgacc ttggagggcc agtgaacaaa gccgcttatg cattctgcct gggcgcaatg
    14221 gcgaacggcg tttacggccc gtatgccatt ttcgcctccg tcaaaatggt ttcggcattt
    14281 accgtaaccg cttccacgat gctcgcaccg cgcctgttta aagagtttga aattgagacc
    14341 gggaaatcca cctggctgtt agggctggca ggtattaccg aaggggcgat cccgatggcg
    14401 attgaagatc cgctgcgggt tattggttcg tttgtgctgg gctctatggt aacgggcgct
    14461 atcgtcggcg cgatgaatat cggcctttcg acacccggtg ccggcatttt ctcgctcttt
    14521 ttacttcatg ataatggcgc gggcggtgtt atggcggcaa ttggctggtt tggtgcggca
    14581 ttggtggggg ctgcaatctc gactgcaatt cttctgatct ggcggcgtca cgcggttaag
    14641 catggcaact atctgactga tggcgtaatg ccataaacaa aaaggaaacg acgatgaaag
    14701 cggtatctcg cgttcacatc accccgcata tgcactggga tcgggagtgg tatttcacca
    14761 ccgaagagtc acgcattctg ctggtcaata atatggaaga gatcctgtgc cgactggaac
    14821 aggacaacga atacaaatat tacgtactcg acgggcaaac ggcgatcctc gaagattatt
    14881 tcgcggtgaa accggaaaac aaagaccgtg tgaagaaaca ggtagaagca ggcaagttga
    14941 ttatcggccc ctggtatacc cagaccgata ccacgattgt ttctgcggaa tccatcgtcc
    15001 gtaatctgat gtacggaatg cgtgactgcc tcacgtttgg cgagccgatg aaaataggtt
    15061 atttaccaga ttcctttggc atgtccgggc aactgccgca tatttacaat ggatttggca
    15121 ttacccgcac catgttctgg cgcggatgtt ctgagcgcca cggtactgat aaaaccgagt
    15181 ttttgtggca aagcagtgac ggtagcgaag tgacggcgca ggtgctgccg ctgggctacg
    15241 ccatcggtaa gtacttacct gccgacgaag acggattacg caaacgcctc gacagttatt
    15301 ttgatgtgct ggaaaaagcg tcagtaacca aagagatttt gctgccgaat ggtcatgacc
    15361 agatgccatt gcagcaaaat atcttcgaag tgatggataa gctacgtgag atctaccctc
    15421 aacgtaagtt tgtgatgagc cgctttgaag aggtatttga gaagatcgaa gcgcagcgag
    15481 ataatctggc aaccctgaaa ggggaattta ttgatggcaa atatatgcgc gtgcatcgca
    15541 ccatcggttc tacgcgtatg gatatcaaaa ttgcccacgc gcgtattgaa aataagattg
    15601 ttaatctgct ggaaccgctg gcaacgctgg cctggacgtt gggttttgaa taccaccacg
    15661 gcttgctgga gaaattgtgg aaagaaattt taaaaaacca cgcccatgac agcatcggct
    15721 gctgctgtag tgacaaagtt catcgcgaga ttgtcgcccg tttcgaactg gcagaagaca
    15781 tggcggataa tctgctgcgt ttctatatgc gtaaaattgc cgataacatg ccacagagtg
    15841 atgccgacaa acttgtgctg tttaacctga tgccctggcc gcgggaagaa gtgatcaaca
    15901 ccactgtgcg gctgcgcgcc agccagttta atttgcggga cgatcgcggt cagcctgtac
    15961 cgtattttat tcgccatgcc cgtgagatcg atcctggcct aatcgatcgg caaatcgttc
    16021 attacggtaa ttatgatccc tttatggagt ttgatattca gatcaaccag attgtccctt
    16081 ctatgggcta tcgcacgctt tatatcgaag cgaatcagcc tggcaacgtg attgcggcaa
    16141 aaagtgacgc tgaagggata ctggaaaatg ctttctggca aattgcgctc aatgaggatg
    16201 gaacgctaca actggtagat aaagacagcg gtgtgcgcta tgaccgggtg ttgcaaattg
    16261 aagaaagctc tgatgatggt gatgaatatg actattcacc cgcaaaagaa gagtgggtaa
    16321 ttacttcggc gaacgcgaaa ccgcaatgcg agattactca tgaggcctgg cagagtaggg
    16381 ctgttatccg ctatgacatg gcggtgccgc gtaatctgca tgaacgcagc gcaagacaat
    16441 ccactggcag agtaggggtg gagttggctg tcactctgag tgataacagc aggcgcattg
    16501 atgtggatat caaccttgat aaccaggctg acgatcatcg ccttcgcgtc ctggtcccta
    16561 caccttttaa caccgacagt gttctggccg atacccagtt tggttcgcta acgcgccccg
    16621 tgaacgacag tgcaatgaac aactggcagc aagaaggctg gaaagaagcg ccggttccgg
    16681 tatggaatat gctcaactat gctgccttac aggaagggcg taacggtctg gctgtcttta
    16741 gcgaagggct acgtgaatat gaagtcatcg gtgaagagaa gaaaaccttt gccattacgt
    16801 tgctgcgtgg cgtgggctta ctggggaaag aagatctgct tttaaggcct ggacgtcctt
    16861 caggaataaa aatgccggtg cccgattcac aattacgtgg ctcctttagc tgccgtctga
    16921 gcttatttag ttatacaggt acgccttcga ccgccggtgt agcccagcag gcgcgcgcat
    16981 ggctgacgcc agtacagtgt tacaacaaaa tcccgtggga tgcgatgaag ttaaataaag
    17041 ccggattcaa tgtgccggaa agctatagct tgctgaaaat gccccctgtg ggatgcctga
    17101 ttagcgctct taaaaaagcg gaagaccggc aagaagtggt attgcgactg tttaatccgg
    17161 cagaatcgac aacctgtgag gcaactgtcg cgttcagtcg tgaggtgatt tcatgtacgc
    17221 aaacgatgat ggatgaaagc atcagcacag cggaaagaga taatttaaag gaactggggg
    17281 catttttacc ggggcagtca cggacattta gctaccgcct tggctgatta ttgcgctaaa
    17341 agtgtatctg aggggggcaa taatttcagg ccatcttcgg atggcctgag attgttgata
    17401 aacaagtttg ttagcttacg cagataccag atttgacctg cgtgagaacc gcaggccatt
    17461 tatcatttca gttacccctt aaacgttaac cacggtcgaa atctgaccgc tggctgaatt
    17521 aatccggctt cttgttgggc ttcgataacc ggaccaatat ctttataagc cgctggcgct
    17581 tcttcaaaac gtcgttcttc gcgcaatgtg acgcattgcc acggcagcgt tgactcttca
    17641 gtcgcgcgtg agcgcatggc ctgccgacgc tggctgcgtc ctgctccgtg actgcatgac
    17701 cacagccact ccgggttgcc gcaacctacc gcaagccacg aataatcgcc cattgaaccg
    17761 gggattaacg ccagattgcc cactcgtgcc ggtgttgcgc ctttgcgatg aatattcatt
    17821 tcatgctcgc gaaggatgat gttatgtggc acatccacca ccagatgcga attgtcctga
    17881 tgaaagagat tggcaagaca tacgcgcacc atttcggcaa ttgtgacgcg atttaaccat
    17941 gcgtaacgcg ccgccattcc tatcgcttgt agatattcct ccgccagtcc gcctgccaga
    18001 ccatataacc cgctttgtgg atgtttatgt ccggtgggcc attcagcacg ggcgcgatcc
    18061 atccagtgtc ttccgacgta aaagccaaca tctcgcgatc cactgtggat catcaccaca
    18121 atatcgcctg ttttcagccc ttgttgccag gctacatggc gatcaaccac gctatcaacg
    18181 acctgaagtt caataaaatg attacctgaa cccggcgtac ccagacaagg atcgcggagt
    18241 aatgcgcggt ccgttgcctg cagggcgggt ggggcataac gcgaatgact gttatgctcg
    18301 ttaagcccaa tgcagcggtc aatctcttgt accattcggg ggatattcgt gcgtatccag
    18361 agaccttctg atggcaaggt cgacacgcaa tctgccggtc cgccatcaaa aagcgcggtg
    18421 aacgcctgtg cggataacgg aacatcgcgc tcattttgca gtagcacttg cgttaaccgc
    18481 tgagcgatga cgtctttatg cgcttcggct tgctcaagtg ttaatccggt cgtcagcagc
    18541 cgcatgccac aagaaatatc ggtgccgatg gcggcgggaa tgactaaatc cggcgttgag
    18601 gccaccaccg cgccaacggg gacgccattg ccgggatgaa aatctggcgt cgcgcacgtc
    18661 tggcgcacat aacctgggtg gtctggtagc ttcacttctg caaaatcaag cagttgtgtt
    18721 actgcttttt cttccagcgg cagttcgtcc ggcagcagta cgcgggcact ggcacctgat
    18781 ggacgagata atgaaacaat attgttggcg cgggaaagtt ggatcccttt gcgcagaaag
    18841 gctttatcaa gccttgaaag atgatgcata acgctgtcct gaaatataga cgttaaacaa
    18901 ccccctgtat ggcgagggta attttgtctt tccggtcagc agccagcaaa agggaaagaa
    18961 aacgcgcgta gtgtatgtgg cgatagcaag tgttacaagt ggtaaatctg gcttattgtt
    19021 aaagaggaat tatcttttgt gaagtctttt aaggccatga ctcattcaat ttaac
//
LOCUS       DAZUCC010000089        18853 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZUCC010000089 DAZUCC010000000
VERSION     DAZUCC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.089"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..776)
                     /locus_tag="ACHY09_002637"
     CDS             complement(<1..776)
                     /locus_tag="ACHY09_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS3885592.1"
                     /translation="MKSFDEQIELVDISDIYIANPRKRNKFIHNEIKENIKTIGLKRP
                     VSVRPVNNENYKYALICGQGRLEAYIEYGETKIPAIIKQVDEETAHLMSLAENIARRN
                     PRAAELMEDIRRMKTSGLTDREIGLRLGYNSSWVNGVITLLSSGEKHLLRAFETGDIP
                     LYLAVEISRSGDEETQELLTVALKEGHIKGGQINKIRRILEQRKKGDKGTISTAFFPG
                     KKQKKLTPEELANVYQENINEQKAILLKSTMVKEKILAVK"
     gene            complement(776..2311)
                     /locus_tag="ACHY09_002638"
     CDS             complement(776..2311)
                     /locus_tag="ACHY09_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS3885593.1"
                     /translation="MSDIVAGNRIAAQYLRMSTEHQKYSPENQREYIEKYAKEHCITI
                     KYTYDDAGRSGLSLDNRPGLRSLLYDVENGIIKVSLILVYDVSRFGRFQDIEEAAHYS
                     WLLKRNGVRIIYCAEPFSEEQTELFMLGLSYSRYGAATYSKNLSQKVFLGQANLVRRG
                     YYQGGMPGYGIRRLLIDEHTNPKGMLLPGQRKSLQTDRVILIPGPAEEVVIVNKIYDM
                     FIDEGKPEFVIASELNRKNIPAENGLQWSRCKIHQILTNEKYIGNNVYNRTSGRLKTR
                     SVRNPQDQWIRCDGAWDPVVSVEKFEAAKRIIEARAVQYSDDELLDKLRHLLKLRGRL
                     SGIVIDEEELFPSSSIFRSRFGGLVRAYTLIGYHPRHDYSYLEIKKQIAECHLTFMDN
                     LVQDIYRYGGWAEMHQDGSLYINDEFFVSANLVRCQRLSSGKLRWKIKFNVSHYCDVM
                     IFPRLDSTNNSVVDYFMFPAIDVLYNDLTLHENNGFILELYRFDDLSCLENLVKRTGL
                     ESK"
     gene            complement(2335..3282)
                     /locus_tag="ACHY09_002639"
     CDS             complement(2335..3282)
                     /locus_tag="ACHY09_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3885594.1"
                     /translation="MRKILFFLLLMIAVLPEVQATCSFYGGVTSEVSGYLNFGNVVIQ
                     RDAQVGSTIATAITGAYNNGDGIAGCKREAWTARWELTQWGTLSGYGNGVYNTNLPGV
                     GLRLSAASTGKTLPYETSYAYNAGGTWVSIPGDGIKGELIKTGDITSGTLTDGVLARA
                     SVVNQFYFANVTLNGMNTVKSEACTVTTPSVDVPMGDHDKSEFSGAGSSTDWVSFDIG
                     LSCDVGARINVRIDATADASASSQGVMLIDSGGASGVGIQLHYRPDDAAVQYGQERFY
                     WQSVYGDEIVQLKARYYQTAGSITPGAANGTATFTMSYK"
     gene            complement(3301..4305)
                     /locus_tag="ACHY09_002640"
     CDS             complement(3301..4305)
                     /locus_tag="ACHY09_002640"
                     /inference="COORDINATES: protein motif:HMM:NF012635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3885595.1"
                     /translation="MRVWRGMILLAGILFCQASWAFCTSSVSFSISPGTVVVQRDAVV
                     GEPISDWLVSNQGMAYQDCNYDATPQYVINAGIKNTGSRASVTWNGEAVFNSNVAGVG
                     FVFEGRSSVDSNPLPENWIGIASGQSQVFTLQISHSVGYHPSINDQARIRLIKTGDIT
                     PGTLSGNLGRFIAGTRENGQWSTEIPITFSGGQITTIACSVTTPDVNVPLGQHQKAEF
                     SGPGTGTGWQAFNIGLDCDKSARINVQIDAVQDPSNVAGVMKLDSAPGDMAATGIGVQ
                     LFFAPDNSAVQFGQSRYYYTSPNGGTETVQLKARYYQTAATITAGVANATATFTLTYK
                     "
     gene            complement(4302..6818)
                     /locus_tag="ACHY09_002641"
     CDS             complement(4302..6818)
                     /locus_tag="ACHY09_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS3885596.1"
                     /translation="MNNPTPRLMIILLTLLLAIVLLSGYLRNAEAREVFNPHALEIDN
                     PGQPVADLSTFAESGGQLPGTYRVTVFVNGEKQSDAQDIAFIDGAKKKLSPQITPAML
                     KAWGVKTDVFPALAALPPDQPLDDIGRYIPMANTELRFSKLQLNVSIPQAAMNASARG
                     YVDPSEWDEGVPAALLNYNLSGSNTWRDGQNGSDDNYYANLQSGINLGAWRLRNYSTW
                     NYDEDNGSHWDSVNTYLQRDIQRLKGQLTLGDSYTPSDIFDSVQFRGAQLASDDNMLP
                     DSLRGFAPVIRGIAQSNAQVTIKQNGYIIYQSYVAPGAFTITDLYPTSGSGDLEVTIK
                     EADGSERTFVQPFSAVPIMQREGRLKYALTAGKYRSGNEDSDEPQFGQVTAIYGLPHA
                     ITVYGGTLYSDDYQSGAAGLGFGLGELGSLSADITAAHTTLNNDETHNGQSYRVQYSK
                     DFQTTDTSFTLAAYRYSTAGFYTFQEANDLRADDDNGWQMTYNKRQRLQLDLSQSIGS
                     YGSFFISGYQQDYWQEDGYERTLSAGWNGNIDGISYSVTWSYSDYPESTQPADQQLAF
                     NIQVPLSRFMPNAWASYSVNTAKHGDTRQQVGLNGTALADNNLSYSLQQSYTNHGVGG
                     SGNINADYKGGQGEITGGYNYDDDTQQVNYGLKGGIVAHPHGITLSQPLGQSLAIVKA
                     PGADDTKVQNNTGVYTDWRGYAVVPYVNPYKKNRIALDTSTLGDEVDTDTAVQTVTPT
                     QGAVVMADFNTRVGRRVLMTLLYRGLPVPFGAEAKLKEGGSGIVGDDGQVYLTGVPEE
                     GDIAVNWNGAQQCVVHYRLPEQENQPSVILVNQECREVAK"
     gene            complement(6830..7510)
                     /locus_tag="ACHY09_002642"
     CDS             complement(6830..7510)
                     /locus_tag="ACHY09_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3885597.1"
                     /translation="MNRYADVVLALLLAFLSVQAQAGIVIGGTRVIYDGDKKETAASV
                     RNPEKSGVYLVQSWVDSGEQGGKTPFIVTPPLFRINPGEENMLRIVRTGGNLPQDRES
                     VFWLNVKSIPATDDSHSRTNVLQVVVKSRLKLFYRPAGLEGQPESAYHQLSVAHSGNH
                     LTVSNPTPYYVTLFTLKVDGQEIKEADMVPPKGSVSFTLPSATASAVTWQAISDYGGV
                     SQTESRKL"
     gene            complement(7624..8136)
                     /locus_tag="ACHY09_002643"
     CDS             complement(7624..8136)
                     /locus_tag="ACHY09_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3885598.1"
                     /translation="MKKTMLSACFLLVTGNAVANDGTIHFTGSITDQTCTVDSGSQNL
                     NVDLGNVGQTALNGAAGMKASPTRFTLNLSGCPDTVTGANVKFDGTSDGVNQNLLALD
                     SGTGIATGVGIEIADKNGAVIPLHTASMDYPLAAGSNTLDFVARYVSTGTAVTTGTAN
                     GTSQFTINYK"
     gene            complement(8504..9094)
                     /locus_tag="ACHY09_002644"
     CDS             complement(8504..9094)
                     /locus_tag="ACHY09_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFS3885599.1"
                     /translation="MPEKRKHLTRHEVGQILLAADRGIHFERDYCLIQMCFLHGLRVS
                     ELCGMRLSDIDLIGRSVYVRRLKNSLSTQHPLFDNELPALMRWLAVRNRWRDADSDWL
                     FLSQKGGALSRHQVRLLLKRYGELAGVSISAYPHMLRHGCGYALADLGRDTRLIQDYL
                     GHRNIRHTVIYTATNTQRFMNVWGNVRENHTISARM"
     gene            9307..10230
                     /locus_tag="ACHY09_002645"
     CDS             9307..10230
                     /locus_tag="ACHY09_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFS3885600.1"
                     /translation="MRAQYILLLLCAAFPLRAITTPEIAASALTPDCVKYRVVGLCYW
                     LYCTPFGCTVRTSVKVSHFRPDLVVSAYSNTGQNPWAEMSLLSPPLPGVAEGGGDTNP
                     RSASQHSKVRFKNADAIGFPAGDELAKFFTQFGYICSPSSQPFQPYFLSQPDTLAWRS
                     GVPEMTYPEALTPGMREVGQNGDMWGNIYPRAGAINQTHDYKAAAVIAQRAADLVTRT
                     GQPHLYTPLTASSRAGYWPPSPVIEGDSDNHRWQMLTPKKSAACSVFPDGSATDTYAN
                     KLAEDGAYAWTLWRPYKCCPRRGQTFLGSTG"
     gene            10242..11603
                     /locus_tag="ACHY09_002646"
     CDS             10242..11603
                     /locus_tag="ACHY09_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFS3885601.1"
                     /translation="MRALFLVLLFFAGVCVAADDYSFRTRGAVDDLLYYQIGGGEAVM
                     PPPVRRSPSPLSLGLGWNADMMCGNFDIDTTVRNQLNGITDGFQQLMGNVIQSATGAV
                     ASLPAMIIQRANPQLYDLLTNGVLQGRLDFDKSLLNCQKMAEKMTDLAADSAWFSGAK
                     AENYQSLAASDNDAIRTDQKAAKEAAEKGKRWVGGEKRGGKGQLPIKVVHDATAAGWN
                     ILNQQPATSTTSLSSSECDGELCSTWTSPEEAAGWMTRVVGEQTISVAQVTDDPDARS
                     GAQAGIGLHPLIDEEKAKILPLLTDLVNGSQKPGFDNLAKASGGSMQLTRGVVEALRD
                     DPDVAVLTERLAGELALARVTEQALLARRTLLAGMREPNIANVKEAQESLGKTTAQLD
                     EELNQLKLELDFRQALTRNTASQILQRKQQRDQLQGQVVEVPDDSDSRLHNLNNPQPD
                     SPR"
     gene            11600..13102
                     /locus_tag="ACHY09_002647"
     CDS             11600..13102
                     /locus_tag="ACHY09_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFS3885602.1"
                     /translation="MTANSYFEYVLTLLGWLVNNGVWQTLTATGLFAVPLLAKLVALW
                     LKARGQGADEGNVARLLVVWVEHSLYSALLVIFFAGVPFLNVDINTLTYDSQRTKQCG
                     WSVVQPGDSGYAPLINELSGQSASVPVWWYFTHALGKGITSAAVAVLPCKPDLRQLRF
                     EVQHTRLADPALAQELQDFVNDCYGPSRARLKRLSSDISEEQSREVDWVGSSFYLATL
                     GYYDRDHSRSPRAAWPYDASRDDGLSDTGGGGYPACKEWWSDGDTGLKNRLLKQVDTT
                     LWQQLQKLGQSKEEYEEAVLRSLVSPRNMQVSQGGLVYNGFGGSAGDNLSQTQLLSRV
                     GGIIGAGLSSYALFPAFDNVRQALPMVQAFLEMALVICIPVILLFSAWDLKTVITLSF
                     VQFALFFLTFWWELARWLDNWLMQMMYDSDTHSYFNLWGLQNTSDDLIVNIIMGVMFL
                     VLPAFWLGALTWAGIRIGAAVAGGMGSAVGDIRSAGEQVGKMIVSKTRIP"
     gene            complement(13247..13447)
                     /gene="glgS"
                     /locus_tag="ACHY09_002648"
     CDS             complement(13247..13447)
                     /gene="glgS"
                     /locus_tag="ACHY09_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006174923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFS3885603.1"
                     /translation="MNRQDINALKNFDFLARSFARMHALGQPVDIDAVTGNMSDEQQA
                     WFRERYDHYRKQAERARVIELR"
     gene            13781..14071
                     /locus_tag="ACHY09_002649"
     CDS             13781..14071
                     /locus_tag="ACHY09_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885604.1"
                     /translation="MSLFDKKPSLMPVVIAPDVWQSAVEFPADPQQVEERLGNLLLVV
                     LLTLRTAGTSRKKVTFTIYCQPPQGDMKVPVALPLSLNYHGHYVQVSMAEQA"
     gene            14137..15042
                     /locus_tag="ACHY09_002650"
     CDS             14137..15042
                     /locus_tag="ACHY09_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFS3885605.1"
                     /translation="MDRSNDIYQKVTDQIIAELENGNVPWLRPWRDGEPPFPINALSG
                     RPYHGINVPLLWNSADRQSFASDRWLTFHQVSALGGQVRRGEKSSLAVLYLPRTQEET
                     DALGQPLLDKDGEPKVRHFGIIREFWLFNLSQCDGLPQALSEPFTRPATPVEMAEAIT
                     MNSGVTLRHRRQSKAYYKPATDLVMMPHPQQFESSEAYYATLLHELTHATGHASRLNR
                     PGIAGAERTEALYAAEELVEEMGSAFLCAHCGIQGRLQHASYIGYWLDILRQDKRAVI
                     RASGMARHASEWLLNAVPQPCQLSA"
     gene            15108..15479
                     /locus_tag="ACHY09_002651"
     CDS             15108..15479
                     /locus_tag="ACHY09_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885606.1"
                     /translation="MGLDLFFFERKKNAPDESQYARVGYFRKVNPLLKWVDKHVGLVR
                     NQVLLEVTKANLQALLSDLQSLTPDNCGAVYPTIDGFFYGYTDYGEHYWKDVRTVTLW
                     VKETLETFDFERDRLMFYAWW"
     gene            15653..17242
                     /gene="mobH"
                     /locus_tag="ACHY09_002652"
     CDS             15653..17242
                     /gene="mobH"
                     /locus_tag="ACHY09_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFS3885607.1"
                     /translation="MLKWLKRPSQSQPPTPDATLPEGWFAPQSAEQLLATPLRQSCLH
                     QLRQLTPLPDTLFDSWILAAIHRFAALVQLLPASQSHHHSTPGGLLDHSLEVACNAIR
                     LRQGYLLPPEAGAEEQARQASAWTVAVVCVALLHDTGKIAVDMEIHQRDGTRWHSWQG
                     YLAQSYRWQYQSHGRDYHLHPAATAMLINQLIPSPLLDWLAGYPAAFASLIYQLTGHY
                     ERSGALAELVQQADKASVARYLGGDIKTALAQKPSSFPQQLLTALRSLVQNEYRLNNP
                     ECGSDGWLTEDALWLISKTTADRVRAWLLQHGVSSVPENNNRLFDELLTHQLIVANED
                     KAIWRCIIRSDKGWSPVEPLTLLRLTPSVIWGQDDQRPACFDGDVTPVIEAEPAPELS
                     APTQPLPEIADDPRLGDNNNFLQWLKNHTSPSATVNTRNGKVHVVENHLFLISPGIFK
                     TYAAETTGDSGDGWKLAQRMFQESGLALRCNDDSFIWICEVRVPQKKSSQLKGFLMED
                     PKQVLGEKVPVNNPWLKLLRE"
     gene            complement(17298..17951)
                     /locus_tag="ACHY09_002653"
     CDS             complement(17298..17951)
                     /locus_tag="ACHY09_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS3885608.1"
                     /translation="MKKKLPDQLSRECIIAAMRAYDEGVRHQFSESRLYDVVFEGRSY
                     PPKAIVGIAASNMTGVEFSPEDFSGGIKSKCVKLLTDQGFQIVEKHLGNEQPAISIFP
                     DELPPQSVFTEGAATQVTVNRYERDRKARQAALAWHGCLCKVCGIDMMKVYGEIAKGF
                     IHIHHLIPLSDIKENYSLDPKKDFIPVCPNCHAMLHRRNPPFSPEELKSIIEYHAAL"
     gene            complement(18063..18329)
                     /locus_tag="ACHY09_002654"
     CDS             complement(18063..18329)
                     /locus_tag="ACHY09_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005289972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885609.1"
                     /translation="MSPRTHRQLVSVEVMWPAQTLPLPLQQVVEALNQGETPDQIIIR
                     MNQRGLLAWREDASAQDTHDIFQVRLDNQNEARFLCRYVTLPLH"
     gene            complement(18456..>18853)
                     /locus_tag="ACHY09_002655"
     CDS             complement(18456..>18853)
                     /locus_tag="ACHY09_002655"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3885610.1"
                     /translation="EFLSPIGDMLKISILGDKMNVTSPKMLSQAIRDARKQRNLTQRE
                     TAASMGMKQSTVSEFENHPEGTSLDTLFKLLAALDLKLQVVSWRGKKTLLMAGSRSGD
                     LWKYEQKGHGAIRTEPPFSLAYHLDFQST"
BASE COUNT         4218 a         5311 c         4968 g         4356 t
ORIGIN      
        1 tgctttactg ccagaatttt ctctttaacc attgtggatt tcagcagaat ggctttttgc
       61 tcattaatgt tttcctggta gacattagcc agttcttccg gggtcagttt tttctgtttt
      121 ttcccaggaa aaaaagccgt actaatcgta cctttatcgc ctttcttacg ttgttcaagt
      181 atgcgcctga tcttattaat ttgcccaccc ttgatatgtc cttctttcag ggcgaccgtc
      241 agcagttcct gagtttcctc atctcctgac cgggatattt ccacagcaag atacaaggga
      301 atatcgccag tctcaaatgc ccgcaaaaga tgcttctctc cggatgacaa cagagtgatc
      361 accccattta cccaactcga attataccca agccgcaagc ctatttcacg atcggtcagg
      421 ccactggtct tcattcttct gatatcttcc attagctctg ccgcccgggg attacgtctt
      481 gcgatgtttt cagccaggct catcaggtgc gctgtctctt catcaacctg cttgatgatt
      541 gcaggaattt tcgtttcacc atactcaata tacgcttcaa gtcgtccctg accacaaatc
      601 agggcatatt tatagttttc attattcact gggcgcacgc taacaggccg tttcagacca
      661 atggttttta tgttctcttt tatttcatta tgaatgaatt tattcctttt ccgtggattt
      721 gcgatataaa tatcactgat atcgacaagt tctatctgtt catcaaaaga tttcattatt
      781 tactctccag accagttcgt tttacaagat tttccagaca tgacaaatca tcaaatcgat
      841 atagttcgag gataaaacca ttgttctcat gaagcgttaa atcgttatac aacacatcaa
      901 tagccggaaa cataaagtaa tcaaccacgg agttatttgt gctatccaac cgtggaaaga
      961 tcatgacatc acaataatgg gatacattaa attttatctt ccaacgaagc ttacctgacg
     1021 acaaacgttg gcagcgaacc agattagcgg aaacaaaaaa ctcatcatta atatataatg
     1081 aaccatcctg atgcatctct gcccaaccgc catatcggta tatatcctgc accaaattat
     1141 ccatgaaagt gagatggcat tctgcaatct gcttttttat ttcaagataa gagtaatcat
     1201 ggcgcgggtg ataaccaata agggtgtaag ccctgactaa cccaccaaac cgtgaacgaa
     1261 aaatgctgct cgatggaaac agttcctctt catcaatcac aatcccggat aatctgccac
     1321 gaagcttcag cagatggcgt agtttatcca gcagttcatc atcactgtac tgaacggccc
     1381 tggcttcaat aatcctttta gccgcctcaa atttctctac tgataccaca gggtcccacg
     1441 cgccgtcaca tctgatccac tgatcctgag gattcctgac acttcgggtc ttcaatcgtc
     1501 cggaggttct gttataaaca ttattgccaa tatatttttc attggtcagt atctgatgaa
     1561 ttttacatcg gctccactgg agcccattct cggcggggat gttcttccgg ttcagttctg
     1621 aagcaataac aaactcaggc ttgccctcat caataaacat gtcatagatt ttattgacta
     1681 ttaccacctc ttccgcgggc cccgggatca atattactct gtcagtttgc aggcttttcc
     1741 tttgccccgg cagaagcatg cctttcggat tagtatgttc atcaattaat aatctccgaa
     1801 tgccatagcc aggcatacca ccttgataat aaccccgccg aacgaggttt gcctggccaa
     1861 gaaaaacttt ttgtgaaagg tttttactgt aagttgcggc gccatagcgg gagtaagata
     1921 atcccagcat aaacaattcg gtctgttctt ccgagaaagg ctccgcgcaa taaataatgc
     1981 gcacaccatt acgcttcagt agccacgagt aatgagcagc ctcttcaata tcctgaaaac
     2041 gaccaaaccg actgacatcg taaacaagaa ttaacgagac ttttattatt ccgttttcaa
     2101 catcatataa cagcgaacgc aggccaggcc tgttatccag gctcagccca cttcggccag
     2161 cgtcgtcata ggtatattta atggtaatac agtgttcttt tgcatacttc tcgatatatt
     2221 cccgctggtt ctccggcgaa tatttctgat gctcggtaga cattcttaaa tattgagcag
     2281 caatcctgtt cccggcgaca atatccgaca tagaatattc catatgatta cgcgctattt
     2341 ataactcatc gtaaacgtcg ccgtaccgtt tgccgcacct ggcgtgatcg agcctgcagt
     2401 ctggtaatat cgcgccttaa gctgcacgat ctcgtcacca tataccgact gccagtaaaa
     2461 ccgttcctga ccgtactgga cagcggcatc atccgggcgg taatgaagtt gtatccccac
     2521 gccagacgcc ccgccgctat caatcagcat gacgccttgc gaactggcgg aagcatccgc
     2581 agtggcatcg atacgtacgt ttattctcgc cccgacatcg cagctcaaac caatatcaaa
     2641 gctcacccag tcggttgaag atcccgcacc actgaattcg cttttatcgt gatcccccat
     2701 cgggacatca acagagggcg tggtcacagt acaagcttct gatttcactg tgttcatccc
     2761 gttcaacgtg acgttggcaa aataaaactg attcactacc gaagcgcgcg ccagcacacc
     2821 gtccgttaat gtaccgctgg tgatatcccc ggttttgata agctcgccct tgatgccatc
     2881 accgggaatg gatacccagg tgccgccagc gttgtaggcg tagctggttt catagggcaa
     2941 tgttttacct gtcgaggccg cactcagacg taatcctacc cctggcaggt tggtgttgta
     3001 gacaccattc ccatagccac tcagcgtccc ccattgcgtc agttcccagc gggcggtcca
     3061 ggcctcccgc ttacacccgg caatgccgtc gccattgttg tatgcccctg taatggcagt
     3121 ggcaatcgtg ctgccgacct gtgcatcccg ctggataacc acgttgccga aattcaggta
     3181 acccgatact tctgatgtca caccaccgta gaacgaacag gtggcctgaa cctccggcag
     3241 gacggctatc atcaacagca ggaagaaaag aatttttctc atctcaacac tccggcgacg
     3301 tcatttatag gtcagcgtga aggtcgccgt ggcattggca acacctgcag taatcgtggc
     3361 tgcggtctgg taataccgcg ccttaagctg cactgtctca gtaccgccat tgggagaggt
     3421 atagtaatag cgtgactgac caaactgtac ggcgctgtta tccggtgcaa agaacaactg
     3481 gacaccgatg cccgtagccg ccatgtctcc gggcgcgctg tccagcttca tcacgccagc
     3541 cacgttggac ggatcctgta cggcatctat ctgaacgttg atacgggcac ttttatcgca
     3601 gtccagcccg atgttaaacg cctgccagcc ggttcccgtt cccggcccgg agaactccgc
     3661 tttttgatgc tgccccagcg gcacattgac atcaggcgtg gtcactgagc aggcgatggt
     3721 ggtgatttgc ccaccggaaa atgtaatggg aatttcagtc gaccactgcc cgttctccct
     3781 tgtccccgcg ataaaacgcc ccaggttgcc gctcagcgta ccgggcgtaa tgtcgccggt
     3841 ttttatcagc cggatccgcg cctgatcgtt aatggaggga tggtagccaa cgctgtggct
     3901 tatctggagc gtgaatacct gcgactgccc gctggcgata ccgatccagt tttccggtaa
     3961 cggattgctg tcgaccgagg agcggccttc aaacacaaat cccacacctg cgacattact
     4021 gttaaacacc gcctcaccat tccaggtgac gctggcgcgg gagcccgtgt ttttgatgcc
     4081 tgcattgatg acgtattgtg gcgttgcatc ataattgcag tcctgatagg ccatcccctg
     4141 gttcgacacc agccagtcgc taatgggttc ccccaccaca gcatcccgct ggaccaccac
     4201 cgtaccgggg ctgatggaga aactcaccga agacgtgcaa aacgcccacg acgcctggca
     4261 gaacaggatc cccgccagca gaatcatccc gcgccatact ctcattttgc cacctccctg
     4321 cattcctggt tcaccaggat cactgacggc tggttttcct gttccggtaa acggtaatgc
     4381 accacgcact gttgtgctcc gttccagtta accgcaatat cgccttcctc cggcacgcca
     4441 gtcagataga cctggccatc atcgccgaca ataccgctgc cgccctcttt cagcttcgcc
     4501 tctgcgccaa aggggactgg cagcccacgg tacagcagcg tcatgagcac acgccgcccg
     4561 acacgggtgt taaaatccgc catcaccacg gcaccctgcg tgggggtcac ggtctgtacg
     4621 gcagtgtcgg tatccacttc gtcgccaagc gtgctggtgt cgagcgcaat acggtttttc
     4681 ttgtacgggt tcacatacgg cactaccgca taaccgcgcc agtcggtgta aaccccggtg
     4741 ttgttctgta ccttcgtatc atccgcaccg ggcgctttca cgatcgccag cgactggccg
     4801 agcggctgcg aaagcgtgat gccatgcgga tgcgcgacaa taccgccctt cagcccgtag
     4861 ttaacctgct gcgtgtcgtc gtcatagttg tagcctccgg tgatttcccc ctgcccgcct
     4921 ttatagtcgg cgttgatatt gccggagccg cccacaccat ggttggtgta gctctgctgg
     4981 aggctgtagc tcaggttgtt gtcggcaagc gccgtaccgt tcagccctac ctgctggcgg
     5041 gtatcgccgt gtttcgcggt gttgacgctg tagctcgccc aggcgttcgg cataaagcgg
     5101 ctgagcggca cctggatgtt gaacgccagt tgctggtcgg caggctgcgt gctctccggg
     5161 tagtcgctgt aactccaggt aacgctgtag ctgataccgt cgatattccc gttccagccc
     5221 gcgctcaacg tgcgttcgta gccgtcctcc tgccagtaat cctgctggta gccggagata
     5281 aaaaacgagc catagctacc gatactctga ctgagatcta actggaggcg ctggcgtttg
     5341 ttgtacgtca tctgccagcc gttatcatca tcagccctca ggtcgttggc ttcctggaag
     5401 gtgtaaaatc cggcggtgga gtagcgatat gccgccagcg tgaagctggt gtcggtggtc
     5461 tgaaaatcct tcgaatactg cacccggtac gactgcccgt tgtgggtttc atcgttattg
     5521 agcgtggtat gggcggcggt gatatcggct gacagcgaac cgagttcacc gaggccaaaa
     5581 ccgagccctg cggcgccaga ctgataatcg tccgagtaca gcgtgccgcc atagacggtg
     5641 atagcatgcg gcagaccgta aatcgcagtg acctggccga actgcggctc gtcgctgtcc
     5701 tcattgccgc tgcggtattt cccggcggtc agggcatatt tcaggcggcc ttcacgttgc
     5761 ataatcggca ccgccgagaa cggctggacg aaggtgcgct cgctgccgtc ggcctctttg
     5821 atggtcactt ccaggtcacc gctgcccgag gtcggataca gatcggtgat ggtaaacgca
     5881 cccggcgcga cgtagctctg gtagatgatg tagccgttct gcttaatggt cacctgagcg
     5941 ttagactggg cgataccgcg gatcaccggg gcgaacccgc gcaggctgtc cggcagcatg
     6001 ttgtcgtcag atgccaactg cgcgccgcga aactggacgc tgtcgaagat gtccgacggc
     6061 gtgtagctgt cgccgagcgt cagttgtccc ttcaggcgct ggatatcccg ttgcaggtac
     6121 gtgtttactg aatcccagtg gctaccatta tcttcgtcat aattccaggt cgagtagttg
     6181 cgtagccgcc acgcgccgag gttgataccg ctttgcaggt tcgcgtagta gttatcatcc
     6241 gaaccgtttt gtccatcgcg ccaggtgttg ctgccgctga ggttgtagtt cagcagtgcg
     6301 gcggggacac cttcgtccca ttcggacgga tccacgtatc ctctggcgct ggcattcatt
     6361 gccgcctggg gaatactgac gttcaactgc aatttactga agcgcagttc agtgttggcc
     6421 atcgggatat agcggccaat atcatccagt ggttgatccg gcggcagtgc tgccagtgcc
     6481 gggaagacgt ccgttttgac gccccaggct ttcagcatcg caggcgttat ctgcgggctg
     6541 agtttctttt tggctccgtc aataaaagca atatcctgtg cgtcgctctg cttttcaccg
     6601 ttcacgaaga ccgttacgcg gtaggtacca ggtagctgac caccggattc cgcaaaggtg
     6661 gataaatccg ccaccggctg gccggggtta tcaatctcca gcgcgtgggg gttaaacacc
     6721 tcacgcgcct cggcattacg caaatagcca gacaaaagca ctattgccaa caacagcgtc
     6781 agcaggatta tcatcagacg tggcgtcggg ttattcatca cgtcctcctt cacagcttac
     6841 ggctttcggt ctggctgaca ccgccatagt cgctgatggc ctgccaggtc accgcgcttg
     6901 cagtcgctga cggtagcgtg aaactcacac tgcctttcgg cggcaccata tccgcctctt
     6961 taatctcctg cccgtcgacc ttcagggtga acagcgtgac gtaatacggt gtgggattgc
     7021 tgacggtaag atggttaccg ctgtgggcaa cgctcaactg gtgataggca ctctccggct
     7081 gcccttccag gcctgccgga cggtaaaaca gtttcagccg cgacttgacc actacctgca
     7141 acacgttggt gcgcgaatgg ctgtcgtcgg tagcggggat cgatttcacg ttcagccaga
     7201 acaccgactc cctgtcctgc ggcaggttgc ctccggtgcg cacaatgcgc agcatgttct
     7261 cttcgccggg attaatgcga aacaacggtg gcgtgacgat aaacggcgtt ttgcccccct
     7321 gttcgccact gtctacccag ctttgcacca ggtagacgcc ggatttctcc gggttgcgta
     7381 ccgaggcggc cgtctctttt ttatccccgt cataaatcac gcgcgtaccg ccaatcacga
     7441 tcccggcctg cgcctgtacg ctcaggaaag ccagcaacag tgccagcacc acgtctgcat
     7501 acctgttcat cttcttgttc tccggacgaa aaatacgcat ccctgcgaaa cagtccctgt
     7561 tatgcggcgg tacaccaggt cgggtaggat gggagaggtg gtgaaccccg cgacggcaca
     7621 gcgttatttg tagttaatgg tgaactgcga ggtaccgtta gctgtacctg tggttaccgc
     7681 ggtaccggtg gaaacatagc gggcgacgaa gtccagcgta ttgctgcctg ccgcgagggg
     7741 gtaatccatt gaggccgtat gcagaggaat gactgcacca tttttatcgg cgatttcgat
     7801 accaacgccg gtcgcaatcc cggtaccgct gtccagcgcc agcaggttct ggtttacgcc
     7861 atcagacgtc ccgtcaaact tcacgttcgc cccggtaacg gtatcagggc aaccactcag
     7921 gttgagcgtg aagcgcgtcg gggaggcttt catcccggcc gcaccgttca gtgcggtttg
     7981 ccctacattg cccagatcga cgttcaggtt ttgcgaaccg ctgtccacgg tgcatgtctg
     8041 gtcggtaata ctgccggtaa aatggatggt gccatcattg gcgaccgcat tcccggtcac
     8101 cagtaaaaaa cacgcgctta acatcgtttt tttcatacgt ttctccttga tgaaagatca
     8161 ctcagggctc aaccctgatt cactctggtg acgagaaaac ccgcgtaaga ctttcccgtt
     8221 agtgccagag tggtgagttc aggagaaaat gtggtggacg aagggggtga aaaataaaat
     8281 gtcagtggaa gtgaagtatg gctttcaaaa tggcataaaa ccgtaatgcg gtgagtgagt
     8341 aaaaactggc aacgtgaacc acttaagcac gttgccagcc aggaccagag agagactaca
     8401 tatttgttac attaaatatt attttaaaaa caacaagata gtccgttacc aaaaggggtc
     8461 tcagaaaaat tttgacagtt tgctaaattt ttctgatact actttacatt ctggcactaa
     8521 ttgtgtggtt ttctctcacg tttccccaga cattcataaa cctctgcgta tttgtcgccg
     8581 tataaatcac cgtatgccgg atgttccggt gccccagata atcctgtatc agacgggtgt
     8641 cgcgccccag atctgccagc gcgtaaccgc aaccgtgtct cagcatatgc ggataagcgg
     8701 aaatgctgac acctgccagt tctccgtaac gtttgagtaa caatctaacc tgatggcgcg
     8761 acagcgcacc acccttctgc gacaggaaca accagtctga atccgcatcc cgccagcggt
     8821 tgcgtaccgc cagccagcgc atcagcgcag gcagttcatt atcaaacagc ggatgctgcg
     8881 tggagagact gtttttcaac cgccggacat acaccgagcg cccgatcaga tcgatatcag
     8941 acaggcgcat cccgcacagt tcactgaccc gcaggccgtg taaaaaacac atctgaatga
     9001 ggcaataatc gcgctcaaaa tggatccctc tgtctgctgc aagcaatatc tgtcccactt
     9061 catgacgagt gagatgcttt cgtttttcag gcatcagcct tgctccttat caaaataaaa
     9121 aatgaaaaaa aatcaggcca gaactgcctt cacagtccag gcctgaacca gagaatacca
     9181 ccagaaacaa cgtctggtga tgatgtaaaa ctcaacaatt ttctgtaacg gcttccagtt
     9241 tcataccgtt gatgaacaga tattgcggta cacccgacag aagaaatatc tgctgtcgga
     9301 ggcgcgatgc gagcccaata catccttctg ctgctgtgcg ctgctttccc cctcagggcg
     9361 attactacgc cggagatcgc agcctctgcg cttacgccgg actgtgtgaa ataccgggtg
     9421 gtcggcctgt gctactggct gtactgcacg ccgttcggct gtacggtacg cacgtcggtg
     9481 aaggtcagcc atttccgccc ggatctggtg gtgtcggctt acagcaacac cgggcagaat
     9541 ccgtgggcgg agatgtcgtt actcagccca ccgttgcccg gtgttgccga aggcggcggc
     9601 gatacgaatc ctcgttctgc cagccagcac agcaaggtcc gctttaagaa tgccgatgcc
     9661 atcggcttcc cggcgggtga tgaactggca aaatttttca cccagtttgg ctatatctgc
     9721 tcaccttcat cgcagccttt tcaaccgtac tttctgagtc aacccgatac gctggcgtgg
     9781 cgcagcggtg tgccggaaat gacatacccg gaggcgctga cgccgggcat gcgggaagtc
     9841 ggtcagaacg gcgatatgtg gggcaatatc tacccgcgag ccggagccat caaccagacg
     9901 cacgactata aagccgccgc cgttatcgcc cagcgcgcgg ccgatctggt gacccgtacc
     9961 gggcaaccgc atctctacac gccgctgacc gcctcatcgc gcgccgggta ctggccgccc
    10021 tccccggtta tcgaaggcga cagcgataac catcgctggc aaatgctcac gccgaaaaaa
    10081 tccgccgcct gttcggtgtt cccggatggc agtgccactg acacctacgc caacaaactg
    10141 gcggaggatg gcgcgtacgc ctggacgctg tggcggccct ataaatgctg tccgcgccgg
    10201 gggcaaacct ttctcggcag tacgggttaa cggaggcggt gatgagagcc cttttcctgg
    10261 tcttattgtt tttcgccggg gtttgcgtgg cggcagacga ctacagtttc cgcacgcgag
    10321 gcgcggttga cgacctgctt tactaccaga ttggcggcgg tgaagcggtc atgccgcctc
    10381 cggtacgccg tagcccctcg ccgcttagct taggtcttgg ctggaacgcc gatatgatgt
    10441 gcggcaattt cgacatcgac accaccgtgc gcaaccagct caacggcatc accgacggct
    10501 tccagcaact gatgggcaac gttatccaga gtgccaccgg ggcggtcgcc agcctgcccg
    10561 cgatgattat ccagcgcgcc aatccgcagc tttatgatct gctgaccaac ggcgtgctgc
    10621 aggggcggct ggattttgat aagtctctgc tgaactgcca gaaaatggcg gagaagatga
    10681 ccgatttggc ggccgattcc gcctggtttt ccggagccaa agcggagaac taccagtccc
    10741 tcgccgccag cgataacgat gccatccgca ccgaccagaa agcggcaaaa gaggccgccg
    10801 aaaagggtaa acgctgggtg ggcggtgaaa agcgcggcgg caaaggccag ctgccgatta
    10861 aggtagtcca tgacgccacc gccgcaggat ggaatatcct caaccagcag cctgccacca
    10921 gcactacttc tcttagctcc tctgaatgcg acggggaact ttgtagcacc tggacatccc
    10981 ctgaagaggc cgccgggtgg atgacccgcg tcgttggcga gcagaccatc agcgtggcgc
    11041 aggtcaccga cgatccggat gcgcgttccg gcgcgcaggc ggggatcggc ctgcatccgc
    11101 tgatcgacga agaaaaggcg aagatcctgc ccctcctgac agatctggtt aatggcagtc
    11161 agaaacccgg cttcgacaat ttggcgaaag cttccggcgg ttctatgcaa ctgacccgcg
    11221 gcgtggtcga ggcgctgcgc gacgatccgg acgttgccgt cctcaccgaa cggctggcgg
    11281 gagaactggc gctggcccgc gtcaccgagc aggcgttgct ggcccggcgt acgctgctcg
    11341 caggtatgcg tgaacccaat atcgccaacg tcaaagaggc gcaggagagc ctgggcaaaa
    11401 ccaccgccca actggatgaa gagctgaacc agttgaagct ggaactcgat ttccgccagg
    11461 cactgacgcg caataccgcc agccagatcc tgcaacgcaa acagcagcgc gaccagcttc
    11521 agggacaggt ggtggaggtg ccggacgaca gcgacagccg tctgcacaac ctcaataacc
    11581 cgcagccgga cagcccgcga tgaccgccaa cagctatttc gagtacgtgc tcaccctgct
    11641 cggctggctg gtgaataacg gcgtgtggca gacgctgacc gccaccgggc tgttcgccgt
    11701 gcccctgctg gcaaaactgg ttgccctgtg gctgaaagcg cgcggccagg gggccgatga
    11761 aggcaacgtc gcccgcctgc tggtcgtctg ggtggagcac tcgctctaca gcgcactgct
    11821 ggtgatcttc ttcgcgggtg tgccgttcct caatgtcgat atcaacaccc tcacctacga
    11881 cagccagcgc acaaagcagt gcggctggtc ggtggtacag cccggcgatt ccggctacgc
    11941 acctttgatt aatgaactca gcggccagag cgcttccgtt ccagtgtggt ggtacttcac
    12001 gcatgcactg ggcaaaggca tcaccagcgc cgccgtcgcc gtattgccct gcaagccgga
    12061 cctgcgccag ttgcgctttg aggtgcagca cacccggctc gccgatccgg cactggcgca
    12121 ggagctacag gatttcgtta acgactgcta cgggccgtcg cgggcaaggc tcaagcggct
    12181 gagtagcgat attagcgagg agcaaagccg ggaagtggac tgggtgggat cgtcttttta
    12241 cctcgccacg ctaggctatt acgaccgcga tcactcccgt tcgccccgcg ccgcctggcc
    12301 ttatgacgcc agccgcgacg acggtctgag cgatacgggt ggcggtggct atcctgcgtg
    12361 taaggagtgg tggtccgatg gcgatacggg gctgaaaaac cgcctgctga agcaggtcga
    12421 taccacgctc tggcagcaac tgcaaaaact gggacagtct aaagaggagt atgaagaagc
    12481 ggtactgcgc tcgctggtca gcccgcgcaa tatgcaggta tcgcagggcg gactggtgta
    12541 caacgggttt ggtgggagtg cgggggataa cctcagccag acgcagttgc tgagtcgtgt
    12601 gggcgggatc atcggcgcgg ggctttcgag ctacgccctg tttccggcgt ttgataacgt
    12661 ccgccaggcg ctgccgatgg tgcaggcgtt cctcgaaatg gcgctggtga tttgtattcc
    12721 agtaattctg ctgttctcgg catgggatct taaaacggtc atcacgctga gctttgtgca
    12781 gttcgccctg ttctttctca ccttctggtg ggaactggcc cgctggctgg ataactggct
    12841 gatgcagatg atgtacgaca gcgataccca ctcatacttc aacctctggg gcctgcaaaa
    12901 cacctccgac gatctgattg taaatatcat tatgggcgtg atgttcctgg tgttacccgc
    12961 attctggctg ggggcgctga cgtgggcggg catcaggatc ggcgcagcag tcgctggggg
    13021 gatgggcagc gcggtcggcg atatccgcag cgctggcgaa caggtcggga agatgatcgt
    13081 cagtaaaaca cgcattccct gaagatttcg tactgacggt gtgatgaaca gggcttgtag
    13141 ctgagtttat gcgcaccgca taaactgtcg tgtcagtgac aatctcgtgg taggtagctg
    13201 gagtctgaaa aaacgcaacg ggccgttaag cccgttgttc gttacatcac cgcagttcta
    13261 tcactctcgc ccgctcagcc tgctttcggt agtggtcgta tcgctcccgg aaccatgctt
    13321 gctgttcatc actcatattg ccggtaacgg catcgatatc aactggctgg cccagggcgt
    13381 gcatacgggc aaaactgcgc gccagaaaat caaaattctt taatgcgttt atatcttgtc
    13441 ggttcattag catatccctc cagtcattgt gtttactgtt caaggtcata tgcttttctg
    13501 atttcagtat ccgcctgaaa aagatcgttt tttgagcgat gcgtgcgcat caatctacct
    13561 gtgttcacat ttcaggaacg acaaaactct tcagccccag taatgatttt cccctccctt
    13621 ttatcacccg cgcagaaaac actaccgcca gccatgcagt aagttcgcca tgtatccgga
    13681 aggtgcgaac ggcgcagatg cgcaggatac ccgtaccatt ccatcccgca gggaaacgcc
    13741 ctgcgaaggg tgttccctgc tcatttacag gagaaacacc atgtcgcttt tcgataaaaa
    13801 accgtcgtta atgccggtgg ttatcgcccc tgatgtctgg caatccgcag tggaatttcc
    13861 cgccgacccg cagcaggtgg aagaacggct gggcaatctg ttgctcgtcg tactgctgac
    13921 cctgcgcacc gccgggacat cccgcaagaa agtcaccttt acgatctact gccagccgcc
    13981 gcagggcgat atgaaagtcc ctgtcgcatt accgctatcg ctcaactatc acggccacta
    14041 tgtgcaggtc tcgatggctg aacaggcata acctctcccc gccggagtcg cttccggatg
    14101 gaggaggctc cggcttcttt ccatgaggac cacacgatgg acagaagcaa cgatatctac
    14161 cagaaagtca ccgaccagat tatcgctgag ctggaaaacg gcaatgtgcc gtggctccgc
    14221 ccgtggcgcg atggcgagcc gccgttcccg atcaatgcgt tatccggtcg tccgtatcac
    14281 ggcatcaatg taccgctgct gtggaacagc gccgacaggc agagcttcgc cagcgaccgc
    14341 tggctgacgt tccatcaggt cagcgcgctc ggcgggcagg tgcgccgggg ggagaaatcc
    14401 tctctggcgg tgttgtatct gccgcgcacg caggaagaga ccgacgcact cggccagccg
    14461 ttgctggata aagacggcga gccgaaggtg cggcatttcg ggattattcg tgaattttgg
    14521 ctgtttaacc tgtcgcagtg cgacggcctg ccgcaggcgc tgtccgagcc gtttacccgg
    14581 cccgcaacgc ctgtggagat ggctgaagcc attacgatga atagcggtgt gacgctgcgc
    14641 catcgccgcc agtcgaaagc gtattacaaa cccgcgaccg atctggtgat gatgccgcac
    14701 ccgcaacagt ttgaaagcag cgaggcgtac tacgccacgc tgctgcatga actgacgcac
    14761 gctaccggac acgcttcacg ccttaaccgc cccggtattg ccggagcgga acgcaccgaa
    14821 gcactctacg ctgctgaaga gttagtggag gagatgggta gcgccttcct gtgcgcccat
    14881 tgcggcattc agggcaggct ccagcacgcc agctatatcg gctactggct ggatatcctg
    14941 cgccaggaca agcgggcggt gatccgcgcc agcggtatgg cgcgccacgc cagtgaatgg
    15001 ctgctcaacg ctgtaccaca accctgtcag cttagcgcct gacagcccac ccgcacgggg
    15061 agccttcccc gccgggaagt tgctcccctt tttctgagga tcaatgtatg ggactcgatc
    15121 tcttcttttt cgaacggaaa aaaaacgcgc ccgatgaaag ccagtacgcc agagtgggtt
    15181 atttccgcaa ggtgaatccg ctgcttaaat gggtggataa acacgttggg ctggtgcgca
    15241 accaagtgct gttagaagtc accaaagcaa atttgcaggc gcttctgagc gacctgcaga
    15301 gcctgacgcc ggataactgc ggcgcggttt atccgacgat tgacggcttt ttctatggtt
    15361 ataccgatta tggagaacac tactggaaag acgttcgcac cgtcactctc tgggtgaagg
    15421 agacgctgga gacgtttgat tttgagcgag acaggctgat gttttatgcc tggtggtagt
    15481 attaaagtgg ccgacaaaac gtcggtcact ttgcctcgtc ggccagaaat acagcgccag
    15541 atatacacat gagagcatac cgtttttact aataaaaatg taaaaggccc aaagggagac
    15601 actgcaaagg aagggcgaca agggcaaagg ggcctgcccg gaaaggggaa atatgctgaa
    15661 gtggctaaaa agaccatccc agagccagcc acctacgcca gacgctacgc tgccggaggg
    15721 ctggtttgcg ccacaatccg cagaacaatt gctggcgacg ccgttacgcc agtcctgttt
    15781 gcaccagtta cggcaactga ccccgctgcc ggataccctg tttgatagct ggatcctcgc
    15841 ggcgattcat cgcttcgccg cgttggtaca actgcttccc gcctcacaaa gtcatcacca
    15901 ttccactccc ggcgggttgc tggatcattc gctggaggtt gcctgcaacg ccatccgact
    15961 gcgccagggt tatctgctac caccggaagc gggcgcggaa gaacaggccc ggcaggccag
    16021 cgcatggacg gtcgcagtgg tctgcgtggc attactgcac gatacgggaa aaatcgccgt
    16081 ggatatggag attcatcaac gtgatggcac ccgctggcat tcgtggcagg gttatctggc
    16141 acagtcttat cgctggcagt accagagcca cggtcgcgac tatcatctgc atccggcggc
    16201 taccgcaatg ctgattaatc agttgatacc ctcgccatta ctggactggc tggccggata
    16261 cccggcggct tttgcgtccc tgatttacca gctcaccgga cactacgaac ggtctggcgc
    16321 actggctgaa ctggtgcagc aggccgataa agcctctgtc gcccgatacc tcggcggcga
    16381 tatcaaaacc gcgctggcgc aaaagccctc ttccttcccc cagcaacttc ttacagcctt
    16441 gcggtcgctg gtacagaacg agtaccggct caataatccc gaatgcggat ccgatggctg
    16501 gctgacggag gatgcgctgt ggcttatcag caaaaccacc gccgaccggg ttcgcgcctg
    16561 gctgctccag catggagttt ccagcgtgcc ggaaaacaat aaccgcctgt ttgacgaact
    16621 gctgacgcat cagcttattg ttgccaacga agataaagcg atttggcgct gcattatccg
    16681 atcggataaa ggctggtcgc ccgttgaacc gctgacgcta ctgcgcctga cgccgtctgt
    16741 aatctggggg caggatgatc aacgtccggc ctgtttcgat ggtgatgtaa cgccggtaat
    16801 tgaagccgaa cccgctcctg aacttagcgc accgactcaa ccgttaccgg agatagcaga
    16861 cgatccgagg cttggtgaca ataacaattt cctgcagtgg ctgaagaacc acactagtcc
    16921 ttcagccacg gtgaataccc ggaacggcaa ggtacatgtg gtggaaaacc atcttttctt
    16981 gatttcgccg gggatcttta aaacatacgc agcagaaacc acaggcgata gcggcgacgg
    17041 ctggaaactg gcgcaacgaa tgtttcagga gtcagggctg gccctgcgct gtaatgatga
    17101 cagttttatc tggatctgcg aggttcgggt tccacaaaag aaaagtagtc agttgaaagg
    17161 gtttctgatg gaagatccga agcaggtgtt gggggagaaa gtgccggtga ataatccgtg
    17221 gctgaagttg ctgcgcgaat agctatttat atcaaaatac tctcttccac cgagctacag
    17281 cgaatgggct ttaataccta aagagccgca tgatattcaa taattgattt caattcttca
    17341 ggcgaaaaag gcggattacg ccgatgcaac atggcgtggc aatttgggca gacgggaata
    17401 aaatcttttt tgggatccag agagtaattc tctttgatgt ctgatagcgg aatcaggtgg
    17461 tgtatatgga taaaaccttt cgctatctcg ccatacacct tcatcatatc aataccacat
    17521 actttgcaca gacaaccatg ccaggctaat gcggcctgtc gggccttacg gtcgcgttca
    17581 tagcgattaa ccgttacctg cgtagctgca ccttcagtaa atacgctctg cggtggcagt
    17641 tcatcaggaa aaatactgat ggcgggctgc tcgttaccca aatgtttctc aacgatctgg
    17701 aacccttgat cggtaagcaa cttgacgcat ttcgacttga taccaccact aaaatcttca
    17761 ggggaaaact caacgccagt catatttgaa gcagcaatac cgactatagc tttcggtgga
    17821 taactgcgcc cttcaaagac cacatcatac agccgggact cgctgaactg gtggcgtaca
    17881 ccttcatcat aggcacgcat agccgcaata atgcattccc tggaaagctg gtctggcagt
    17941 ttcttcttca tttacaactc atcatccctg aaacgacatt tgctaactct ttattttata
    18001 gcatattaat ttatccggtg ataaacagca ctcttcaatt tcgcgttacc gggatagggg
    18061 catcagtgca gcggcaacgt aacgtagcgg cagagaaatc gcgcctcatt ctggttgtcc
    18121 agacgtacct gaaagatatc gtgtgtatcc tgcgcggatg catcctcacg ccacgccagc
    18181 agaccccgtt ggttcatgcg gataataatc tgatcgggcg tttcgccctg attcaatgct
    18241 tcgactacct gttgtagggg aagcggtagc gtctgagcag gccacatcac ttcaacactt
    18301 acgagctggc ggtgtgtccg aggtgacatc ggtggtttcc tccatggctg gaatttagcg
    18361 gttaattagc catgaaatag gggcattttt tactgtttca actactgccg agatggctac
    18421 ggaatacgcg ctgactgatg caaaaatagc ttctcttatg tgctctggaa atccagatgg
    18481 tacgctagtg aaaatggtgg ttccgtccgg atagcgccat ggcctttttg ttcatatttc
    18541 cacagatcac cactcctgct cccagccatc agaagagttt ttttacccct ccagcttacg
    18601 acctgaagct taaggtccag agcggcaagc agcttaaaga gcgtatccag gctggtcccc
    18661 tcaggatggt tctcaaactc tgaaaccgtt gactgtttca tgcccatact tgctgcagtt
    18721 tcacgctggg tcaggttacg ttgcttgcgt gcatccctga ttgcctgaga caacattttg
    18781 ggtgaggtta cgttcatttt atcccccagg atagatattt tgagcatatc ccctataggg
    18841 gataaaaatt cat
//
LOCUS       DAZUCC010000090        18823 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZUCC010000090 DAZUCC010000000
VERSION     DAZUCC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.090"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..1108
                     /gene="tqsA"
                     /locus_tag="ACHY09_002656"
     CDS             74..1108
                     /gene="tqsA"
                     /locus_tag="ACHY09_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFS3885611.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLQQYRNSIM
                     TPLKAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(1133..2521)
                     /gene="pntB"
                     /locus_tag="ACHY09_002657"
     CDS             complement(1133..2521)
                     /gene="pntB"
                     /locus_tag="ACHY09_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFS3885612.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATILGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(2532..4064)
                     /gene="pntA"
                     /locus_tag="ACHY09_002658"
     CDS             complement(2532..4064)
                     /gene="pntA"
                     /locus_tag="ACHY09_002658"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFS3885613.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFTAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            4588..5532
                     /gene="ydgH"
                     /locus_tag="ACHY09_002659"
     CDS             4588..5532
                     /gene="ydgH"
                     /locus_tag="ACHY09_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFS3885614.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            5718..7100
                     /gene="ydgI"
                     /locus_tag="ACHY09_002660"
     CDS             5718..7100
                     /gene="ydgI"
                     /locus_tag="ACHY09_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3885615.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWS
                     ITGAGILLLTFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            7137..7853
                     /gene="folM"
                     /locus_tag="ACHY09_002661"
     CDS             7137..7853
                     /gene="folM"
                     /locus_tag="ACHY09_002661"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFS3885616.1"
                     /translation="MGKAQPLPILITGGGRRIALAWHFINQKQPVIISYRTHYPAIDG
                     LINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLTDVLAC
                     MMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALDNM
                     TRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYL
                     LTSCFVTGRSFPLDGGRHLR"
     gene            complement(7850..8185)
                     /gene="ydgC"
                     /locus_tag="ACHY09_002662"
     CDS             complement(7850..8185)
                     /gene="ydgC"
                     /locus_tag="ACHY09_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFS3885617.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            8314..9033
                     /gene="rstA"
                     /locus_tag="ACHY09_002663"
     CDS             8314..9033
                     /gene="rstA"
                     /locus_tag="ACHY09_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFS3885618.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILQE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            9037..10338
                     /gene="rstB"
                     /locus_tag="ACHY09_002664"
     CDS             9037..10338
                     /gene="rstB"
                     /locus_tag="ACHY09_002664"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFS3885619.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAVESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            10414..11343
                     /gene="tus"
                     /locus_tag="ACHY09_002665"
     CDS             10414..11343
                     /gene="tus"
                     /locus_tag="ACHY09_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFS3885620.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKTAVRLPGVLCYQV
                     DNLSQAALVSHIQHINQLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(11340..12743)
                     /gene="fumC"
                     /locus_tag="ACHY09_002666"
     CDS             complement(11340..12743)
                     /gene="fumC"
                     /locus_tag="ACHY09_002666"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFS3885621.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRVFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGG"
     gene            complement(12886..14532)
                     /gene="fumB"
                     /locus_tag="ACHY09_002667"
     CDS             complement(12886..14532)
                     /gene="fumB"
                     /locus_tag="ACHY09_002667"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFS3885622.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            14731..15906
                     /gene="manA"
                     /locus_tag="ACHY09_002668"
     CDS             14731..15906
                     /gene="manA"
                     /locus_tag="ACHY09_002668"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFS3885623.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISECYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            16007..17515
                     /gene="ydgA"
                     /locus_tag="ACHY09_002669"
     CDS             16007..17515
                     /gene="ydgA"
                     /locus_tag="ACHY09_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFS3885624.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(17560..>18823)
                     /gene="uidC"
                     /locus_tag="ACHY09_002670"
     CDS             complement(17560..>18823)
                     /gene="uidC"
                     /locus_tag="ACHY09_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFS3885625.1"
                     /translation="RKIVAMAVICLTAASGLTSAYAAQPADDEAGLRIRLKNELRRAD
                     KPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDK
                     SFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGY
                     WALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTS
                     RYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETAL
                     VSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNY
                     RLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKGR
                     EKRDGSALGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         4627 a         4889 c         4554 g         4753 t
ORIGIN      
        1 gtttgatttt cgttatgatg acggagcgaa aaagacatta ttattagcaa aggaagaaaa
       61 aacggggaca agcatggcaa agccgatcat cacgctcaat ggcctaaaaa tcgtcattat
      121 gttgggaatg ctggtcatta ttctctgcgg tatccgtttt gccgccgaga tcatcgtgcc
      181 gtttattctc gcattattta ttgctgttat tcttaacccg ctggtgcaac acatggtccg
      241 ctggcgtgtg ccgcgtgtac tggcagtgtc aattttgatg accatcatcg tgatggcgat
      301 ggtgttgctg ttagcttatc tgggttccgc gctcaatgag ttgacgcgga cgttacagca
      361 atatcgcaac tctattatga cgccactgaa agcgattgaa ccgttgttgc aacgcgtagg
      421 gattgatgtc tcagttgacc aactggcgca ttacattgat ccgaacgcgg cgatgacgtt
      481 gctcaccaac ttattgacgc agttatctaa tgccatgtca tcaatctttt tattgctgct
      541 gacggtgctg tttatgctgc tcgaagtgcc acaattgccc ggaaaatttc agcaaatgat
      601 ggcgcgtccg gttgaaggga tggcggcgat tcagcgtgcg attgacagtg tgtctcatta
      661 tctggtgctg aaaacagcca tcagcatcat caccggcctg gtcgcatggg cgatgctcgc
      721 cgcactcgat gttcgcttcg cttttgtctg gggattgctg gcctttgcgc ttaattacat
      781 cccgaatatt ggttcagttc tcgcggcaat cccccctatt gctcaggtac tggtgtttaa
      841 tggcttctac gaagcgttgc tggtgctggc gggatatctg ctgattaatc tggtcttcgg
      901 caatattctg gagccgcgca tcatgggacg tgggctgggg ctttcgacat tggtggtatt
      961 tttgtcgttg attttttggg gatggttgtt aggaccggtg ggtatgctgc tttccgtgcc
     1021 gttgacaatt attgtcaaaa ttgcgcttga acaaacagcg ggaggtcaaa gcatcgccgt
     1081 tctgttaagc gatctcaata aagagtgacg gcctccgcag aggccgtcag ggttacagag
     1141 ctttcaggat tgcatccacg ctggctttgg cgtcaccaaa cagcatgtgg gtgttttcct
     1201 tgaagaacag cgggttttgc acaccagcat agccagtgtt catcgaacgt ttaaagacaa
     1261 tcacgttctg cgctttccac acttccagca caggcatacc agcaatagga ctcttcggat
     1321 catcctgcgc cgccgggtta accgtatcgt tagcaccaat caccagtacg gtatcggtat
     1381 cagcaaagtc atcgttgatc tcgtccattt ccagcacgat gtcatacggt acttttgctt
     1441 cagccagcaa tacgttcata tgtccaggca aacgccccgc aaccgggtgg ataccgaaac
     1501 gcacgttgat accacgagcg cgcagtttct cggtaatttc agcgacagga tattgcgcct
     1561 gcgcgactgc catgccgtac cccggagtaa tgatcactga atgggagttt ttcagcagtt
     1621 ccgctgtctc ttctgcggtg atttcgcggt gctcgcccac ttcctgatca tcgccagtag
     1681 aagagccgtc ggtgccgaaa ccacccgcaa taacgctgat aaaggaacgg ttcatcgcct
     1741 tacacataat gtaagaaagg atagcgcccg aagaaccgac cagcgcaccg gtcacaatca
     1801 gcagatcgtt gctgagcata aagcccgcag ccgcagccgc ccagccggag tacgagttca
     1861 gcatcgacac caccactggc atatctgcac caccgatgga ggcgactaaa tgccagccga
     1921 ataccagcgc aatggcggtc attatcagca atgccagcac ttgcaggccg acgctgtccg
     1981 tgcgaacaaa tacaatcagc agcaggaagg aaacgaccag agccgccagg ttcattttgt
     2041 gacggtttgg cagcatcaac ggtttagacg aaatcttgcc acacagtttg ccgaacgcca
     2101 ccaccgaacc cgtgaacgtt accgccccga tgaagatacc gaggaacact tccgtcaggt
     2161 gaatattgac cagaatcggt gccattcccg cgtcatgatg cagatagctg ttaaagccaa
     2221 ccagcactgc cgccagaccc acgaagctgt gcaggatcgc caccagttct ggcatttcgg
     2281 tcatttcaac tttcttcgcc agccggatac caattgcccc accaatgacc atcgccagca
     2341 agatccagcc aacattaccc gtatccggtc ccaaaatggt tgcgattaac gcaatcgcca
     2401 tcccggcgat accgaagttg ttaccctggc gagacgtttc atgtttcgaa agaccggcca
     2461 gactgaagat aaacaggatc gcggcaacaa tgtatgcagc tgtaactaat cctccagaca
     2521 tatgttaccc cttaattttt gcggaacatt ttcagcatgc gctgagtcac ggtgaagcca
     2581 ccgaaaatat taatgctggc tataagcacc gcgataaaac taaggaagct aacccagccg
     2641 ccctggccaa tctgcaacag tgctccgaca acaataatcc ctgaaatcgc gttggtgacc
     2701 gacatcaacg gtgtatgcag cgcgtgcgat acattccaca ccacgtaata accgacaaca
     2761 caggccagcg caaaaacggt gaagtgccca agaaactctt tcggcgcaac gcttgccaac
     2821 cagccgaaaa gaatgattgc cagcgccatc aacgcgtatt tacgccacgg tgagcaggca
     2881 catttttcct cggttttcac ttccggtgcc gctttttgtg ccgcctgcgg ctgagctgat
     2941 acctgaatcg gcggtgccgg ccaggtaatt tcgcccgcac ggatcacggt cacgccgcga
     3001 atcaccacat catcaaaatc aacagtgata ttgccgtctt tctctttgca caacagtttc
     3061 agcagattaa cgaggttagt accgtaaagc tgtgaggatt gcgtcggcag acggcccgga
     3121 agatcggtat aaccaatcac tttgacacca ttttccgtag tgaagatttc acccggcacg
     3181 gtgtattcac agttgccgcc gttttgggct gccaggtcga caatcacact acccgccttc
     3241 atggagtcaa ccatttcacg ggtaattagc ttcggcgctg gtttgcctgg aataagcgcg
     3301 gtggtgacaa tgatatcgac ctcttttgcc tgggcggtaa agagttccat ttccgctttg
     3361 atgaacgcgt ccgacatcac tttggcatag ccatcgccgc tgcccgcttc ctctttaaaa
     3421 tccagctcga ggaattccgc gcccatactt tgaacttgtt ctttcacttc cgggcgggtg
     3481 tcgaatgcac gcacaatcgc gccgagactg tttgcagcgc caatggcggc cagacccgca
     3541 acacccgcac caatcaccat cacttttgcc ggtggcactt tcccggccgc agtaatttgc
     3601 ccggtaaaga agcgcccaaa ttcatgtgcc gcttcaacaa tggcgcgata accggcgata
     3661 ttcgccatcg agcttagtgc gtccagcgat tgtgcgcgtg agatacgcgg cacagagtcc
     3721 atcgccatca cggtcacgtt acgttccgca agtttttgca ttaattccgg attctgcgca
     3781 ggccagataa aactcaccag cgttgtccca ggattcagta acgcaatttc atcatctaac
     3841 ggcgcattga ccttcagaat gatctctgac tgccagacgc tattcccttc tacaatttca
     3901 gcgcccgctt gcacaaacgc tttatcgtca aaacttgcca gttgacccgc gccgctctct
     3961 accgcgacgg taaaacccag tttcagcagc tgttccactg tttttggcgt tgctgcaaca
     4021 cgggtttcat tggttaaccg ttctcttggt atgccaattc gcatgatatt cccttccatc
     4081 ggttttattg atgatggttt gcctgtgtca ggagccacac aagccgctca tgtacgagct
     4141 aaatgttact ccgttaaaat aaattagtaa caaacgccct ataacgtact gaaaattatg
     4201 cctgtgatct agcgccaaaa atcagtattt cggcgtgaac tcgcaaaata ttaacgattc
     4261 agccgtgata gtgggataaa caccttagaa cgccggataa agactgataa ttgtcttcgg
     4321 cgttcgcata aaacgagaca tcgccccggc acgaatcact acttaacatt aaattaactt
     4381 atacaattca gttgcttcag tagtattgat gctgatacgg ctgtttttta agcatagacg
     4441 gccatttgag caggattaaa attggcttaa ggaatgtgat atgaaaaatg acgcagacag
     4501 ttacaccgtt taaatgcaat aatcagccac gtttctcgtt aataacaata ccagtacctg
     4561 gtttgcgcaa ggcgaaggat tatttttatg aagcttaaga acaccctcct ggcgtcggca
     4621 ctgctttctg ctacggcatt ctccgttaac gcagcaacag aactgacacc ggagcaagcg
     4681 gcagcggtta aaccttttga ccgtgtagtg gttaccggtc gttttaatgc tattggcgaa
     4741 gcggtgaaag ccgtttctcg tcgcgctgat aaagaaggtg ccgcctcttt ttatgttgtc
     4801 gacacttctg attttggtaa cagcggtaac tggcgtgtgg tcgctgacct ctataaagcc
     4861 gatgctgaaa aagcagaaga aacaagtaat cgcgtaatta acggtgttgt cgaactgccg
     4921 aaagatcagg ctgttctgat tgaaccgttt gacacggtca ccgtccaggg cttctatcgt
     4981 agccagccag aagtcaatga tgccatcacc aaagcggcaa aagcgaaagg tgcctactct
     5041 ttctacatcg ttcgtcaaat cgatgccaac cagggcggca accagcgtat tactgcattc
     5101 atctataaaa aagatgctaa gaaacgtatc gtccagagcc cggatgtgat cccggcagat
     5161 tccgaagcag gacgtgcagc tctggctgcc ggtggcgaag ctgcgaagaa agttgagatc
     5221 ccgggtgttg cgactaccgc atcaccaagt tctgaagtcg gtcgcttctt tgaaacccag
     5281 tcatcaaaag gcgggcgtta cactgtcacg ctcccagacg gcactaaagt cgaagaactg
     5341 aacaaagcga ccgcagcgat gatggtcccg ttcgacagca tcaaattctc tggcaactac
     5401 ggcaacatga ctgaagtctc ctatcaggtt gcgaaacgtg ccgcgaagaa aggtgctaag
     5461 tattaccaca tcacccgcca gtggcaagaa cgtggtaata acctgaccgt cagcgcagat
     5521 ctgtataaat aactattcag ttactcctat aaggcggctt gatgccgcct ttcgcatttt
     5581 tagtcacaaa tttccccgcg accattgcat gcagacacat ctcttcgtaa aatcccgcgc
     5641 ttcagtggca aactccattt ttatgcgttt tgccctaata tttattcttc ttgtcacgtt
     5701 ttaatcactg gatatcgatg gaaaagaaac tgggactgag cgcactcacc gcgctggtat
     5761 taagctcaat gctgggcgcg ggtgttttca gtctgccgca aaatatggcg gcagttgcca
     5821 gcccggcagc actgctcatc ggctggagta ttactggtgc tggtatttta ttactgacct
     5881 ttgccatgct gatcctcacg cgcattcgtc ccgaacttga cggcggtatc tttacctatg
     5941 cccgcgaagg atttggtgag ctaatcggct tttgttccgc atggggatac tggctgtgcg
     6001 cggtcatcgc caacgtctcc tatctggtga tcgttttttc cgcgttaagc ttttttacgg
     6061 acacgccgga attgcgcctg tttggcgatg gtaatacctg gcaatcgatc gtcggtgcat
     6121 cggctttatt gtggattgtt cactttttga tcctgcgcgg agtgcaaacc gctgccagca
     6181 ttaacctggt ggcgacactg gcaaaattgt tgccgctggg tctgtttgtt gtgctggcga
     6241 tgatgatgtt caaactggat accttcaagc tcgacttcac cggacttgca cttggcgtac
     6301 ccgtttggga acaggtgaaa aacaccatgc tgatcaccct gtgggttttc attggtgtgg
     6361 aaggtgccgt ggttgtttct gcgcgtgcgc gtaataaacg tgatgttggc aaagcgacac
     6421 tgctggcggt tctctccgct ctgggcgttt acttgctggt aacgctgctt tcgctgggcg
     6481 tggtggcacg tcctgaactg gctgaaattc gtaacccgtc aatggccgga ctgatggtgg
     6541 aaatgatggg accgtggggc gaaatcatca tcgctgccgg tttgatcgtt tccgtttgcg
     6601 gtgcgtactt aagctggacc atcatggcgg cagaagttcc gttcctggca gccactcata
     6661 aagcattccc gcgcattttt gcgcgccaga acgcacaagc tgcgccatcc gcttcactgt
     6721 ggctgaccaa tatctgcgtg caaatttgtc tggtactcat ctggctgacc ggttccgatt
     6781 acaacacatt gctgaccatc gcctcagaaa tgattctggt gccctatttc ctggtcggcg
     6841 cgttcttgct gaaaatcgca acccgtccac tgcataaagc ggtaggtgtc ggtgcctgca
     6901 tttatggctt atggttattg tacgcctctg gtccaatgca cttgctgtta tccgttgttc
     6961 tgtatgcacc ggggctgctg gttttcctgt acgcgcgtaa aacgcatacc catgataatg
     7021 tactgaatcg tcaggaaatg gtgctgattg gcatgctgtt aattgcctca gttccggcga
     7081 cctggatgct ggtgggataa cgttcatccc atcgttggat agcaaaggag ataacgatgg
     7141 gtaaagccca acccttgcca atattaatta ctggcggagg tcgtcgcatc gccctcgcct
     7201 ggcatttcat taatcaaaag caaccggtga ttatcagcta tcggacgcac tatcccgcca
     7261 ttgatggact gattaacgca ggtgcgcagt gtattcaggc tgatttttcg accaacgacg
     7321 gtgtgatggc gtttgccgat gaagtactaa aaagcaccca tggcctgcgt gctattttgc
     7381 ataacgccag cgcgtggatg gcagaaaaac caggtacacc actgaccgac gttctggctt
     7441 gcatgatgca gatccacgtt aataccccat acctactcaa ccatgcactg gaaagattac
     7501 tgcgtgggca cggacacgcc gccagcgata tcattcactt taccgattat gtggtggagc
     7561 gcggtagcga caaacatatt gcctatgctg caagcaaagc ggcactggat aatatgaccc
     7621 gctcgtttgc ccgcaagctg gcaccggaag tgaaagtgaa ttctattgcg ccatcgctga
     7681 tcctgtttaa tgagcatgat gatgccgaat accgacaaca ggcgctgaat aaatcactga
     7741 tgaaaaccgc gcctggcgag aaagaagtga tcgacctggt cgattactta cttaccagct
     7801 gctttgtcac cggacgcagt ttccctcttg atggcggtcg tcatctgcgt taatgcagct
     7861 ttatccagca tataatcaac acccatgcgc ttatccccca gcatgcaaca gaaccaacaa
     7921 aagcggcggg caagcgcatc attccggtga aataccacag cgacgccagg taaacaaaat
     7981 agggaataat cgaccacatg ctaaaaatga tggttgcgcg taaggcttca atgccgcgtt
     8041 cgctggcaac aatataatgc gcgatgagcg caaaggtcgg gaaaagtggt atcagcccgg
     8101 cgatataata atttttcgtt tttgctaaaa caccaatcaa cagcactacc agcgcaccga
     8161 gcgcggcttt gattaccagc cccatctttt taccttaaca cttccataac aagttatcag
     8221 tagaatacct gatgaaacct tgtttagaaa cgattgatag taagtaaaaa cagcgcggtg
     8281 tattgtgacg tttttatatc taccgtgaat gttatgaaca ctatcgtatt tgtggaagat
     8341 gatgcggaag tcggttcact gattgccgcg tacctggcaa aacatgatat gcaggttacc
     8401 gtggaaccgc gcggcgaccg ggccgaagaa actattctgc aagaaaatcc ggatttggtg
     8461 ttactcgaca tcatgctacc aggcaaggac ggcatgacca tttgtcgtga tttacgcgca
     8521 aagtggtccg gaccgattgt tcttctaacc tctctcgata gcgatatgaa ccacatcctg
     8581 gcactggaaa tgggtgcctg cgactatatt ctcaaaacga cgccccctgc tgttttgtta
     8641 gcgcgtttac gtttgcattt gcgtcagaat gagcaagcca cactgagcaa aggtcttcag
     8701 gaaacgtctc tgactcccta caaagccctg catttcggca cgttgaccat cgatcctatc
     8761 aaccgcgtag tcaccctggc taacactgaa atctcgctct cgacagctga tttcgaatta
     8821 ttgtgggaat tagctaccca tgctgggcaa atcatggacc gcgatgcatt gctgaaaaat
     8881 ttacgcggcg tcagttatga cggactggat cgtagcgtgg acgtggctat ttcgcggtta
     8941 agaaaaaaac tgctcgataa cgccgcagaa ccttatcgca ttaaaactgt tcgtaacaaa
     9001 ggctatcttt ttgcgcctca tgcatgggaa taagcgatga aaaaactgtt tatccagttt
     9061 tacctgttat tgtttgtctg cttccttgtg atgtctctgc tggttgggct ggtgtacaaa
     9121 tttaccgccg aacgcgcggg caaacagtcg ctggatgatt tgatgaacag ttcgctgtat
     9181 ctgatgcgca gcgaattgcg tgagatcccc ccacacgatt ggggtaagac gctgaaggag
     9241 atggatttaa atctctcttt cgatctgcga gtcgagccac tgagtaaata ccatcttgat
     9301 gatatttcca tgcaccggct gcgtggcggc gaaattgtcg ccctggacga tcagtacact
     9361 tttttgcaac gtatcccgcg cagccactac gtgctggcag ttggtcctgt tccttatctt
     9421 tattacctcc atcagatgcg attactggat atcgccctga tcgcttttat tgccatttcc
     9481 ctcgcctttc cggtgtttat ttggatgcgt ccgcactggc aggatatgtt aaaactggaa
     9541 gcagcggcgc aacgatttgg cgatgggcat ctcaatgaac gtatccactt tgatgaaggt
     9601 tcgagctttg aacgacttgg cgtcgcattt aaccagatgg cggacaatat caacgccttg
     9661 attgccagca aaaaacagct tattgacggt atcgctcacg aactgcgaac accgttagtg
     9721 cgcctgcgtt atcgactgga gatgagcgat aacctgagcg ccgtcgaatc ccaggcgttg
     9781 aatcgcgata tcagtcaact tgaagcttta attgaagagc tgctgactta tgcccgactc
     9841 gatcgcccac aaaatgagct tcatcttagc gaaccagacc tgccgttgtg gctgtcaacg
     9901 catctggcag atattcaggc agtaacgccc gataaaacgg tacggataaa aacgctcgcg
     9961 caaggccatt atgctgcgtt ggatatgcgc ttaatggagc gcgtgctgga taatttgctc
    10021 aataacgccc tgcgctactg tcactcaacg gttgaaacca gcctgctact gtcggggaat
    10081 agagcgacat taattgttga ggatgatggc ccagggattg cccctgaaaa ccgcgaacat
    10141 atctttgaac cttttgttcg cctcgatccc agccgggatc gctcaaccgg cggctgcggg
    10201 ctggggctgg caattgtcca ctctatagca ctggcaatgg gcggtacggt taattgtgac
    10261 accagcgaac tgggtggtgc ccgcttctcg tttagctggc cgttatggca taacatcccg
    10321 caatttacct ctgcttgaca ttacgcgcac ggtggtcaag tcaccaccac tgtgctataa
    10381 aataaggatg ttgtaactaa agtggttaat attatggcgc gttacgatct cgtagaccga
    10441 ctcaacacta cctttcgcca gatggaacaa gagctggctg catttgccgc tgagctagaa
    10501 cagcacaaat tgctggttgc ccgcgtgttc tctttgccgg aggtaaaaaa agaggatgag
    10561 cataatccgc ttaatcgtat tgaggtaaag caacatctcg gcaacgacgc gcagtcgctg
    10621 gcgttacgtc atttccgcca tttatttatt caacaacagt ccgaaaatcg cagcagcaag
    10681 accgctgttc gtctacctgg cgtgttgtgt taccaggtcg ataacctttc gcaagcagcg
    10741 ttggtcagtc atattcagca catcaatcaa ctcaagacta cgttcgagca tatcgtcacg
    10801 gttgagtcag aactccccac agcggcgcgt tttgaatggg tgcatcgtca tttgccgggg
    10861 ctgatcaccc ttaatgctta ccgaacgctc actgttctgc acgatcccgc cactttacgt
    10921 tttggctggg ccaataaaca tatcattaag aatttgcatc gtgatgaagt cctggcacag
    10981 ctggaaaaaa gcctgaaatc accgcgcagt gtcgcaccgt ggacgcgcga ggagtggcaa
    11041 agaaaactgg agagagagta tcaggatatt gctaccctgc cacaggacgc gaagttaaaa
    11101 atcaaacgtc cggtgaaggt gcagccgatt gcccgcgtct ggtacaaggg agatcaaaaa
    11161 caagtacaac acgcttgccc tacaccgctt attgcgctga ttaatcggga taatggcgcg
    11221 ggcgtgccgg acgttgggga gttgttaaat tacgatgccg acaatgtgca acaccgttat
    11281 aaaccacagg cgcagccgct tcgtttgatc attccacggc tgcacctgta tgttgcagat
    11341 taacccccgg ctttcatact gccgaccatc tgttctggcc gtacccagct gtcaaactcg
    11401 gcttcgctaa gataccccag cgcaagggcc gcagctttta aggtcagccc ttctttatgc
    11461 gcttttttgg cgatctcggc ggctttgtca taaccaatgt gggtgttaag cgcagtcacc
    11521 agcatcagcg attcattgag taattgattg attcgctcac gattcggttc aatgcccact
    11581 gcgcagtgtt tgttaaaact ttccatgcca tctgccagca agcgcaccga ttgcaggaaa
    11641 ttatggatca ccatcggacg gaagacgttc agttcaaagt taccggaagc gccacccatg
    11701 ttgatcgcca catcgttccc catcacctga cagcagagca tggttaatgc ttcacactgc
    11761 gtcgggttca ctttccccgg catgattgag ctacccggct cattttccgg gattgagatt
    11821 tcaccaattc cgcagcgcgg gccagaggcc agccagcgga catcattggc aattttcatc
    11881 agtgacgcag ccaacccttt caacgcgccg tgcgcctgaa ccagggcatc acaggtcgcc
    11941 agcgcttcaa atttgttcgg cgcggtaaca aacggtgcac aagtaatgac tgccagttca
    12001 tctgccacgc gacgcgcata ctccggatgg gtatttagtc cagtacccac cgctgtaccg
    12061 ccaagagcca gttccgctac atgcggcagg ctgtattcga tatgtttcag attatgctcg
    12121 agcatcgcta cccagccgga aatctcctgc cccagcgtta acggcgtggc gtcctgcaag
    12181 tgggtacgac cgattttgac gatatcagca aatacgcggg atttttcact cagcgtctgg
    12241 gtcagggttt taagttgtgg aatgagttgc ttgcgcagcg ccagtagtgc cgcaacgtgc
    12301 atcgccgtcg gaaagacatc gttggaactt tggcttttgt tcacgtcgtc gttagggtga
    12361 actttacgtt ccatcccgcg cacgccaccg agtaattcac tggcccggtt agccagcact
    12421 tcgttcatgt tcatgttact ttgcgtgccg gagccggtct gccagatagc cagcgggaat
    12481 tcgtcgtcat gctgtcctgc cagtacttca tccgccgcct gccgaatggc gctcgctttc
    12541 tcttcagaca acaagcctaa atcttcatta acttttgccg ctgcgcgctt ggttagcgcc
    12601 agcgcatgaa tcagtgaggt gggcattttc tccgtcgaaa tgcggaaatg ctccagcgag
    12661 cgctgagttt gtgcgcccca cagcttatct gccgggacat caatcgctcc catcgaatct
    12721 ttttcgctgc gtactgtatt catggcctgc tcctcacctg attaattttt tctttctgtt
    12781 ttgctttcgt taagcaactt ttagcttact cattatttac catttgataa caaatgtttg
    12841 gtctttcgtg ccatgtaaaa aaaccgcccc gaagggcggc tctgtttatt tcacacagcg
    12901 ggtgcactgt gtgagttgta tctgctggaa gaagtcattt cctttatcat ccacaaggat
    12961 aaatgccggg aaatcttcca cttcaatttt ccagatggct tccattccca gttccggata
    13021 ttcaacacat tccaggctct taatgcttcc ctgcgccaat acagctgccg gaccaccgat
    13081 actgccaagg tagaagccgc cgtgtttttt acaggcatcc gtcacctgct ggctgcggtt
    13141 gcctttcgcc agcatgatca tacttccgcc ctgcgcttgc agttgatcga cataagaatc
    13201 catccgtccg gcggtcgttg ggccaagaga accggaggca taaccttccg gcgttttggc
    13261 cggacccgcg tagtaaatcg gatgatcttt aatatactgc ggcagccctt cgccgttatc
    13321 catccgctct ttcagtttgg cgtgagcaat atcacgacca acgataatcg tgccattcag
    13381 cgataagcgt gtagaaacgg gatactgcga caactgtgcg aggatctctt tcatcggacg
    13441 gttaaggtca acgcgcaccg cttcgccttc tcccgcttta cgcagctctt ccgggatata
    13501 tttgcctgga ttatgttcca gtttttcgat ccagatcccc tgacggttga tcttcgcttt
    13561 gatattacgg tcagcagagc aggagacgcc cataccgacc gggcaggatg cgccgtgacg
    13621 tggcaggcga atcacgcgga tgtcgtgagc gaagtattta ccaccaaact gcgcacccag
    13681 accaagattt tgcgcttcga tcagcaattc tttttccagt tccacatcgc ggaacgcctg
    13741 accgtgctca ttcccttccg ttggcagttc gtcatagtat ttagcggaag ccagtttcac
    13801 cgttttaagg ttcgtttccg cagaagttcc accaattacg aacgcaatat gatacggagg
    13861 acaggccgcc gtacccagcg tgcgcatctt ctcaaccagg taatttttca gtttccccgg
    13921 cgttagtaac gctttggttt cctgatagag atacgtcttg tttgccgaac caccaccttt
    13981 ggcgatacag aggaatttgt actcgtcgcc atcaacggca taaagatcga tctgcgctgg
    14041 tagattggtg ccggtattca cctctttata catatccagc ggcgcgtttt gcgagtagcg
    14101 cagattatct tcgatataag tgttatagac accgcgtgcc agcgccgctt catcaccgcc
    14161 accggtccat acacgctgcc cttttttgcc aacaataatc gcggtgccgg tatcctgaca
    14221 ggttggcaga acgcctttcg ccgcgatgtc ggagttgcgc aggaattgca gcgccacata
    14281 tttatcattt tcgctggctt ccgggtcacg cagaatgtcg gccacctgtt gttggtgagc
    14341 cggacgcagc ataaatgacg catcatgaaa cgcctgacgc gccaacaaag ttaacgcttc
    14401 gggtgcgact ttcaaaatct cctgcccttc aaattcagat acgctaacgt gttcgctggt
    14461 tagcaggtaa tactcagtat catctttttt gagtggaaaa ggagcctgat aatgaaaggg
    14521 tttgtttgac attgttctct cacttactgc ctggtttggt tatgctctgg gcgggtgttc
    14581 cgttgccctg ttaaaagcga gtaacaatat cctacacact tttttaacaa aaactgagac
    14641 tagtacgact ttttgcggct ccaggttact tcccgtagga ttcttgcttt aatagcggga
    14701 ttaatttcca cattaaaaca gggattgatc atgcaaaaac tcattaactc agtgcaaaac
    14761 tatgcctggg gcagcaaaac ggcgttgact gaactttatg gtatggaaaa tccgtccagc
    14821 cagccgatgg ccgagctgtg gatgggcgca catccaaaaa gcagttcacg agtgcagaat
    14881 gccgccggag atatcgtttc actgcgtgat gtgattgaga gtaataaatc gactctgctc
    14941 ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc tgttcaaagt attatgcgca
    15001 gcacagccgc tctccattca ggttcatcca aacaaacgca attctgaaat cggttttgcc
    15061 aaagaaaatg ccgcaggtat tccgatggat gccgccgagc gtaactataa agatcctaac
    15121 cacaagccgg agctggtttt tgcgctgacg cctttccttg cgatgaacgc gtttcgtgaa
    15181 ttttccgaga ttgtctccct gctccagcca gtcgcaggtg cacatccggc gattgctcac
    15241 tttttacaac agcctgatgc cgaacgttta agcgaactgt tcgccagcct gttgaatatg
    15301 cagggtgaag aaaaatcccg cgcgctggcg attttaaaat cggccctcga cagccagcag
    15361 ggtgaaccat ggcaaacgat tcgtttaatt tctgaatgtt acccggaaga cagcggcctg
    15421 ttctccccgc tgttgctgaa tgtggtgaaa ttgaatcctg gcgaagcgat gttcctgttc
    15481 gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg aagtgatggc gaactccgat
    15541 aacgtgctgc gtgcaggtct gacgcctaaa tacattgata ttccggaact ggttgccaac
    15601 gtgaagttcg aagccaaacc ggctaaccag ttgttgaccc agccggtaaa acaaggtgca
    15661 gaactggact tcccgattcc tgtggatgat tttgccttct cgttgcatga ccttagtgat
    15721 aaagaaacca ccatcagcca gcagagtgcc gccattttgt tctgcgtcga aggcgatgca
    15781 acgctgtgta aaggttctca gcagttacag cttaaaccgg gtgaatcagc gtttattgcc
    15841 gccaacgaat caccggtgac tgtcagaggc cacggccgtt tagcgcgtgt ttacaacaag
    15901 ctgtaagagc ttactgaaaa aattaacatc tcttgctaag cttattaaag gcttataaca
    15961 ccttcaggcg gccagtccgc ctgatttcat tttatggata atcattatga ataaatcgct
    16021 ggtagcggta ggcgtcattg ttgcgctagg cgtagtctgg acaggcggcg catggtatac
    16081 aggcaagaag attgaaaccc atctcgaaga catggtcgcg caggcgaacg cgcaactcaa
    16141 actgaccgct cctgaatcca acctggaagt gagttatcaa aactatcatc gcggcgtatt
    16201 cagcagtcag ctgcaactgt tggtgaaacc cattgccgga aaagtaaatc cgtggattaa
    16261 aagcggtcag agcgtcatct tcaacgaatc ggttgatcat ggtcccttcc cgcttgccca
    16321 gcttaaaaaa ctgaatctga tcccgtcgat ggcatcaatt caaaccacgc tggttaataa
    16381 cgaagtaagc aaaccactgt ttgatatggc aaaaggtgaa acgccttttg agattaactc
    16441 ccgcattggt tacagcggag attccagttc cgacatttcg ctcaagccac tgaattacga
    16501 gcaaaaggat gaaaaagtcg cctttagcgg cggcgagttc cagttaaatg cagacagaga
    16561 cggcaaagct atctcccttt ccggggaggc gcaaagtggt cggatagacg ctgttaacga
    16621 atacaaccag aaagtacagt tgacctttaa taatctgaaa accgacggtt ccagcacgct
    16681 ggcaagtttt ggtgagcgcg taggaaacca aaaactgtca ctggaaaaaa tgaccatttc
    16741 agtggaaggc aaagaactgg cactgctgga aggcatggag atcagcggta aatcggatct
    16801 ggtcaatgat ggtaaaacga tcaatagcca actggattac tcgctaaaca gcctgaaggt
    16861 acagaatcag gatctgggca gcggcaagct gactttaaaa gtcggccaaa ttgatggcga
    16921 agcctggcat cagtttagcc agcaatataa cgcgcaaact caggcgctgc tggcacagcc
    16981 agaaattgcc aacaatcccg aactttatca ggaaaaagtg acggaagcct tctttagcgc
    17041 cctgccgctg atgttgaaag gcgatccggt gattactatc gcgccgctaa gctggaaaaa
    17101 cagtcagggt gaaagtgcgc tgaatctgtc gctgttcctg aaagatccgg caacgactaa
    17161 agaagcgccg caaacgctgg cgcaggaagt agaccgttcg gttaaatctc tggatgcgaa
    17221 actgaccatt ccggtggata tggcaactga gttgatgact caggtagcga agctggaagg
    17281 ttatcaggaa gatcaagcga aaaaactggc gaaacagcaa gttgaaggtg catcagcaat
    17341 ggggcagatg ttccgtctga ccaccttgca ggacaatacc atcaccacca gcctgcaata
    17401 tgctaacggt cagatcacgt taaacgggca gaaaatgcca ctggaagatt ttgttgggat
    17461 gtttgcgatg ccggcattaa acgttccggc tgtacccgct attccgcagc agtaattcac
    17521 agccaaaatg ccggagattt ttctctccgg cattttttct tacttcagca taaagtcata
    17581 ctgaatactc attttcacat cccagtaatt caggcgatcg cccagcgcac tgccatcgcg
    17641 cttttcccgc cctttatcgg cagcaagaaa gaaattcatc ccttgtaacg gcccttcgct
    17701 gacccgatag ttcataccac ctaagatacc cgcgccttca atccgcacat ctcgtttact
    17761 ggattcatag ccgcgagtca caatcggtgc aaatgtcagc gtaaattgcg gcgttaaacg
    17821 atagttgaca cccaattgcc aggactgcat tccttcacgg ttacgatcaa gacttaatga
    17881 aaagggatag ccaatgtcgg tatccaccac ggcaccattc attgcatggc gcaaataacc
    17941 accactgcca aatacgcttc cccacggcgc attccaggtc aattgtccac taaccaacgc
    18001 cgtttcattg ggctggctgg tattacgact taaaccttca agttgcgcgt aaaagccgag
    18061 caactcccct ttcaacgtat aattcgggtc aagcggtatg cttttctcga gaatactttg
    18121 tatctgattg gaaacatctt tctgtaccga tgcccccaga gaatagcgac tggtatcatc
    18181 atgccaactg gcggctaaaa aagaacgtgc gcgatgttta tcgtattttc ccgtctggct
    18241 gttatacacc ccttcatcac gaataccggt tgttgaatcg gtccataaaa atactcgtga
    18301 ccgccacatt ccccacagat caatatttgg cgttaaagcc cagtaaccta atgctccttc
    18361 agaaactgtc ggtaatgtac gttgcgaact gccagccatt aacggaatac gataaggtaa
    18421 gctaccataa ctataatccg tcccgaagcg acctaatttt agcaggctat tttcactggg
    18481 ttttattttt actgccccca gggcaaagcc aaaactttta tcaccatcaa gataccaccg
    18541 ggtactcata tcagcacgag cacctaattt ataaacataa tacgccccgc cttcaacgcc
    18601 aataatattg gaataataac cactattgaa atcgagcaat cctccctgta cccatgcgta
    18661 aatatctctt cccgtgccag cactgggctt atcagccctg cgtaattcgt ttttcagtct
    18721 gatgcgtagt cccgcttcat cgtcagccgg ttgcgccgcg taagcagagg taaggccaga
    18781 ggcagccgtc aggcaaataa cggccatggc cactattttt ctc
//
LOCUS       DAZUCC010000091        18381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZUCC010000091 DAZUCC010000000
VERSION     DAZUCC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.091"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..584)
                     /gene="rsmG"
                     /locus_tag="ACHY09_002671"
     CDS             complement(<1..584)
                     /gene="rsmG"
                     /locus_tag="ACHY09_002671"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS3885626.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLHVPALDG"
     gene            complement(648..2537)
                     /gene="mnmG"
                     /locus_tag="ACHY09_002672"
     CDS             complement(648..2537)
                     /gene="mnmG"
                     /locus_tag="ACHY09_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS3885627.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2916..3359)
                     /gene="mioC"
                     /locus_tag="ACHY09_002673"
     CDS             complement(2916..3359)
                     /gene="mioC"
                     /locus_tag="ACHY09_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFS3885628.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3449..3907)
                     /gene="asnC"
                     /locus_tag="ACHY09_002674"
     CDS             complement(3449..3907)
                     /gene="asnC"
                     /locus_tag="ACHY09_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFS3885629.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4059..5051
                     /gene="asnA"
                     /locus_tag="ACHY09_002675"
     CDS             4059..5051
                     /gene="asnA"
                     /locus_tag="ACHY09_002675"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFS3885630.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5056..6507)
                     /gene="viaA"
                     /locus_tag="ACHY09_002676"
     CDS             complement(5056..6507)
                     /gene="viaA"
                     /locus_tag="ACHY09_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFS3885631.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCMALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6501..7997)
                     /gene="ravA"
                     /locus_tag="ACHY09_002677"
     CDS             complement(6501..7997)
                     /gene="ravA"
                     /locus_tag="ACHY09_002677"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFS3885632.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8220..10088
                     /gene="kup"
                     /locus_tag="ACHY09_002678"
     CDS             8220..10088
                     /gene="kup"
                     /locus_tag="ACHY09_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFS3885633.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10255..10674
                     /gene="rbsD"
                     /locus_tag="ACHY09_002679"
     CDS             10255..10674
                     /gene="rbsD"
                     /locus_tag="ACHY09_002679"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS3885634.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPHLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10682..12187
                     /gene="rbsA"
                     /locus_tag="ACHY09_002680"
     CDS             10682..12187
                     /gene="rbsA"
                     /locus_tag="ACHY09_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFS3885635.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12192..13157
                     /gene="rbsC"
                     /locus_tag="ACHY09_002681"
     CDS             12192..13157
                     /gene="rbsC"
                     /locus_tag="ACHY09_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFS3885636.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13182..14072
                     /gene="rbsB"
                     /locus_tag="ACHY09_002682"
     CDS             13182..14072
                     /gene="rbsB"
                     /locus_tag="ACHY09_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFS3885637.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14198..15127
                     /gene="rbsK"
                     /locus_tag="ACHY09_002683"
     CDS             14198..15127
                     /gene="rbsK"
                     /locus_tag="ACHY09_002683"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS3885638.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15131..16123
                     /gene="rbsR"
                     /locus_tag="ACHY09_002684"
     CDS             15131..16123
                     /gene="rbsR"
                     /locus_tag="ACHY09_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFS3885639.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLLHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16089..17516)
                     /gene="mdtD"
                     /locus_tag="ACHY09_002685"
     CDS             complement(16089..17516)
                     /gene="mdtD"
                     /locus_tag="ACHY09_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFS3885640.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17539..18231)
                     /gene="yieP"
                     /locus_tag="ACHY09_002686"
     CDS             complement(17539..18231)
                     /gene="yieP"
                     /locus_tag="ACHY09_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS3885641.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAMVGTAEQKAHLNTLMTEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4422 a         4566 c         4806 g         4587 t
ORIGIN      
        1 ccatccaggg ccggaacgtg aagtttaacc actgattcga cctgatattc ttcgggcaac
       61 aaagcgattt catcttccgg catttggcct ttcagcgcgt agaaacggcc ttgctcacca
      121 ggaagatggt gacaccagct caccatatcg ttcagagagg caaaagcgcg gctaattacg
      181 ccatcaaatg gcggctctga aggaaactct tctaccctgc tctgtactgg ctcaatattc
      241 tccagtttaa gctcatgttg cacctgacga aggaaacgca cgcgtttacc aaggctatcc
      301 aacagagtga aatgggcttc aggacgcacg atagagagtg gaatgccagg cagtcctgga
      361 ccggtgccga catcgataaa ccgttcacct tgcagatacg gtgccaccac aatgctatcg
      421 agaatatggc gtaccagcat ctcattagga tcgcggaccg aagtcaggtt gtacgcttta
      481 ttccatttat gcagcatatt cacataggca ataagctggt ttttctggtg atcggtaagc
      541 gaaatacctg cgtctttcag cagtaaggag agtttgttga gcacggtgat tacctgttct
      601 tgatgcgttg cccggtaagc gggtgcttac caggcatttt taatgcgtta tgcgctacga
      661 cgcagcatac cctgtttttt cagccacacc agcagaatgg agatggccgc aggcgtgacg
      721 ccagaaatac gcgaagcctg gccgatagag gccggtttgt gatcgttaag tttggcgatc
      781 acttcgttag aaagaccgga tacctggcgg taatccagcg tcgctggtag cagggtgttc
      841 tcgttacgca gctgcttttc gatctcatct tgctggcgcg cgatataacc ttcgtattta
      901 acctgaatct caacctgttc cgccgcctgt tcgtctgtca acgcaggggc aaacggcgtc
      961 agcgtggtta atttttcata agtcatttcc ggacgacgca gcagatcttc accactggct
     1021 tcacgggaaa gtggcgcagt caggtgagca ttcacttcgg ctgcagcttc cgccgacggg
     1081 gttacccagg tcgatttcag acgctgacgc tcacgctcga tattctcaag tttctcgtta
     1141 aagcgcgccc aacgttcgtc atccaccagg cccagttcac gaccaatttc agtcaaacgc
     1201 agatccgcat tatcttcgcg cagcatcaga cgatattctg cgcgcgaggt aaacatacga
     1261 tacggttctt tggttcctaa agtgcacagg tcatcaacca gtacgccgag atacgcctga
     1321 gaacgcgccg gagcccaacc ttctttgtca gcagacagac gggcagcgtt aagaccggcc
     1381 agcaaacctt gcgcagcggc ttcttcgtaa ccggtagtgc cgttaatctg accagcaaag
     1441 aacagcccct ggataaactt gctctccagc gtcggtttca ggtcgcgtgg atcgaagaag
     1501 tcatactcaa tggcataacc cggacgcacg atcttcgcgt tttccatccc ctgcatggag
     1561 cggacgattt gcatctgcac atcgaacggc aggctggtgg agataccgtt cggataaatt
     1621 tcgtttgagg tcagcccttc cggttcaagg aagatctgat gctgatttct gtcggcgaag
     1681 cgcatgactt tgtcttcgat cgacgggcag tagcgtgggc cgacaccttc gatcacccct
     1741 gcatacattg ggctacgatc gaggttactg cggatcacat catgggtttt ctcgttggta
     1801 tgggtgatat aacacggcac ctgctgtgga tgctgggacg cattacccat aaacgagaat
     1861 accggcattg ggttatcgcc atgctgttgc gccagcacgc tgaagtcgat agtacgcgca
     1921 tcaatacgcg gcggtgtccc ggttttcaga cgaccaacgc gcagcggcag ttcacgcaaa
     1981 cggcgagaga gcggaatgga cggcggatca ccagcacgac caccgctgta gttatccaga
     2041 ccgatatgaa ttttaccgtc gaggaacgtc ccaacggtaa gcacgacggc tttggcacgg
     2101 aacttcagtc ccatttgggt aacagcaccg accacgcgat cgttttcgac aataagatct
     2161 tcaaccgcct gctggaagat catcaggttc ggttggttct ccagcgccgt acgtaccgcc
     2221 tgacggtaga gcacacgatc cgcctgagct cgggtagcgc gaaccgccgg tcccttgctt
     2281 gcgtttagta tcctaaactg gatacccgcc tgatcgatcg ctttcgccat cagaccgccg
     2341 agtgcatcca cttcttttac cagatgtccc ttcccaatac cgccgatcgc cgggttgcag
     2401 ctcatctgcc ccagagtgtc gatattgtgt gtcaaaagca gagtctgttg acccatacgc
     2461 gccgcggcca tcgcggcctc ggtgcctgca tgacccccgc caatgatgat gacgtcaaaa
     2521 ggatccggat aaaacatggt gattgcctcg cataacgcgg tatgaaaatg gattgaagcc
     2581 cgggccgtgg attctactca actttgtcga cttgagaaag acctgggatc ctgggtatta
     2641 aaaagaagat ctatttattt agagatctgt tctattgtga tctcttatta ggatcgcact
     2701 gccctgtgga taacaaggat ccggctttta agatcaacaa cctggaaagg atcattaact
     2761 gtgaatgatc ggtgatcctg gaccgtataa gctgggatca gaatgagggg ttatacacaa
     2821 ctcaaaaact gaacaacggt tgttctttgg ataactaccg gttgatccaa gcttcctgac
     2881 agagttatcc acagtagatc gcacgatctg tatacttatt tgagtaaatt aatccacgat
     2941 cccagccatt cttctgccgg atcttccgga atgtcgtgat caagaatgtt gatcttcagt
     3001 gtttcgcctg tctgttttgc accggaattt ttgagttctg cctcgagttt atcgatagcc
     3061 ccacaaaagg tgtcatattc acgactacca ataccgattg cgccaaagcg gaccgcagaa
     3121 agatcgggct tctgttcctg caatgcttca tagaaaggag aaaggttgtc cggaatatct
     3181 ccggcaccgt gggttgagct gataaccagc cagatcccag aggcagataa atcttccaac
     3241 agcggaccgt gcagcgtttc ggtggtaaaa cccgcctctt ccagcttttc agccaggtgt
     3301 tctgctacat attcggcacc gccgagggtg ctgccgctga taagagtgat atctgccata
     3361 aaccgccacc tttattaaga gtggcgtatt gtacgctgtg aacgcgttgg gatctacctg
     3421 tggaaaagta tgggattaaa aaagccgatc agggcttgat ggtacgcatg atcgggtttt
     3481 gcaggacgat caatgtctcg gtggactgaa tttcatcaat tgtttggatc ttgttgataa
     3541 gtacatgctg gagagcgtcg atcgaacggc acatcacttt tataaagatg ctgtagtggc
     3601 cggtggtgta ataggcttca gtgacttcat caaggctttc cagtttcgcc agcgcggaag
     3661 gatagtcttt ggcgctcttt aatataatgc cgataaagca gcctacgtca taaccgagct
     3721 gcttcgggct gacatcaata cgcgccccgg taatgatccc cgcctgcttc attttctcta
     3781 ctcgaacgtg aatcgtcccc ggactgacgc caaattgttt cgccagttcg gcgtaagcgg
     3841 tgcgcgcatt gcccattaat gcttccagga tgccacggtc cagattgtcg attagataat
     3901 tttccatagg attttcttat gcggattgat gattcattct attttagcct tcttttttaa
     3961 tgaatcaaaa gtgagttagg ctttttattg aatgattatt gcatgcgtgt cggtttttgt
     4021 tgcttaatca taagcaacag gacgcaggag tataaaaaat gaaaaccgct tacattgcca
     4081 aacaacgtca aattagcttc gtgaaatctc acttttcccg tcaactggaa gaacgtctgg
     4141 ggctgatcga agtccaggcg ccgattctta gccgtgtggg ggatggcacg caggataact
     4201 tgtcgggctg tgaaaaagcg gtgcaggtaa aagtgaaagc tctgcctgat gcccagttcg
     4261 aagtggttca ttcactggcg aagtggaaac gtcagacatt agggcaacac gacttcagcg
     4321 cgggcgaagg gctgtacacg cacatgaaag cccttcgccc cgatgaagac cgtctttctc
     4381 cgttgcactc ggtctatgtt gaccagtggg actgggaacg cgtaatgggc gacggtgagc
     4441 gtcaattctc gactctgaaa agcacggtag aggcgatctg ggcgggaatt aaagcaaccg
     4501 aagctgcggt tagcgaagag tttggcctgg caccgttcct gccggatcag atccacttcg
     4561 tacacagcca ggagttactg tctcgttatc cggatctcga tgccaaaggg cgtgagcggg
     4621 cgattgcgaa agatcttggc gcggtattcc tcgtcgggat tggcggcaag ttgagtgatg
     4681 gtcatcgtca cgacgtgcgc gcaccggatt atgatgactg gagcaccccg tcagagctgg
     4741 gccatgcggg tctgaacggc gatattctgg tatggaaccc ggtactggaa gatgcgtttg
     4801 agctttcttc catggggatc cgcgtggatg ccgatacgtt gaaacatcag ctggcgctga
     4861 ccggtgatga agatcgcctg gagctggagt ggcatcaggc gctgctgcgc ggtgaaatgc
     4921 cgcagaccat cggcggcggt atcgggcagt ctcgtttgac catgctgctg ctgcaactgc
     4981 cacatatcgg ccaggttcag tgtggtgtat ggccggcagc tgttcgcgag agcgtccctt
     5041 ctctgctgta ataatttatc gccgccagcg tctgagcagg cggcttcgca tcccggtatc
     5101 aaagcgccag atatgatcga aaatgcgcat gatgccgggt ttgccatgtg cagacatcgc
     5161 cacggcatga aagcgatgct gatgtacgcg ctgtagctct ttcactttac tcgtcacgtc
     5221 gtcaggcaac cgctgggcga taaaatcaga aatcaccacc gcatcggcat caaaccattc
     5281 cctgctttgc aagcgttcca taatggcgcg aaaacaactg gcaagatcgg tgccgccacg
     5341 aaactgctgg cttaaaaaac ggattgcttg ttcgatgcct tgtgggcctg aaagctcata
     5401 acggacgatc tcggtggaaa ataacataat atagcagcgc cggttttcag cgagagcaat
     5461 gcgcatcaag gccatgcaga acgctttcgc acactgttca ttaaagccgc ccattgagcc
     5521 ggaagtatcc acacagacaa taaacggtcc gcgcggctgt tcgtcgtaat ctttatgtac
     5581 caccgggcgt tcgatcactt tttcacgcca cgactcaccg tgcaggcgat aggtgagcaa
     5641 ctgtttttcc accagccgac ggtaaaactc atactccagt tccgttatcc ccagtgtcgc
     5701 cagttctggc ggcaggagac gtaaaatatc atcgctttgt tgcagaccat caacctgctc
     5761 aggaaccgtc gccggttcgc gcaccatggt gcggaaggtt tccatctgcg catcgttgcg
     5821 cggtattgat ttggcttccc gtgaacgccc caactgttct gccaggcgtt tcagttccgg
     5881 ctgttcgtta agaaattcac cgtatttcac aatcaactga tagtcgccgc gtttaagctg
     5941 accggcgctc atatcccaca gacgaccagc tgcggtattg ttatctgcga gaatcggttc
     6001 aagttgcccg ctcagcgtca tgcgttcctg aacttcactc aacagttgtt cgcgttcttc
     6061 ttctaatagc tgctgattta gcgtcgttgc ttgcacgatc agacttaaac gccagcgctg
     6121 gagaaaaagc gtgtgtaacg ctgaagtgat cgtactgtta gcatcaacca actgtcgggc
     6181 ttgttctgcc catggagaat tcagacgatg cagtaagtcc aggatctgtg gtagctgcac
     6241 aataaactgc ggcgtggaga ggagctggct ttgctgatag cacatcacct cttcggtgag
     6301 ttccggcggg actcgggcat ctttcagccg actgcgcagc gcctcacgcc agcggggaac
     6361 atcatcagtg attgccgcct tcagtcgtgg gaatttttca aagaagaccg ccagctgcgg
     6421 tgaggccagc agcgcgatga tcatctcttc gatcaatccc tcttcgctga cggccagcat
     6481 cacattaagc gtatccagcg ttagcattgt tgtgcctggc gaatctgggc gccgacatcc
     6541 tgtaggctgg cttcaatgcg acctaaccag tcgccaggga taaacagaca tttttgctgt
     6601 tcgctgaata aagcgtgttg cttgcgccag tcgctttcaa gctcttccag ttgttgtttt
     6661 atttcacctg gcagaccttc agccgatgaa ccgggaagtg ccagcgtact gccttgtaaa
     6721 cttacatcgc gtacaacaag atgttgggcg ctatccactt ccagattcaa tttctgggca
     6781 aagccgatac cgttcagttt gccgcgaatt tcaccacctt tactcagcca ctgttccagc
     6841 gcgttacgct caaaggagat atgaaccact tccatatcat gcagttttaa cggtttttgc
     6901 agcagcagag tcagagtgga agcagtaacg ttaacaggaa gttgatactg ctgacgacgg
     6961 ctgaaaatgc cgcccagacg aattaccgtt aaggctgttt tatcgctttg ttgctgctgg
     7021 agttgcaggt gacgttgcac aatcgcgccc aggcgggtca acattccttg ctgttgccag
     7081 gcatgaccgg tcatcaatac atcaatttgt tgttgtatca aattcaggct ttgcgcgtca
     7141 taccacaggc aatctttcag caaaatgaga tcaaccgggg caacagcact gcgaccgcta
     7201 aaaaaggcgc tggcctgcaa taatcgaatc gcttttttcc agcgacgatc cgagacataa
     7261 ggcgcatccg gtaatttatc cagttgctgg cgcagcataa aaatcagctc aaatacatga
     7321 tcgggcagcg taatttcacc aatctctttc tgccagcgtt catattcttc atctgtgacc
     7381 tgcaaggagg caggaaccgg attgtcgttt tcatcctgtt gactggtcag catggagcgg
     7441 aaattcgctt tatcctgcac tttatctaac cacagacgaa tcagcatgcg gtcatataac
     7501 gcttccagac tgctgtctgc ttccggcagc tcgttggagg ccgccaccag caggcgcatc
     7561 gggatttttt ctacgtgtgc accgttgcgg aactggcgct cgttaatggc ggtgagcagg
     7621 gtattaagaa ttgccggacc cgctttccag atctcatcca gaaagacgat ttctgcttcc
     7681 ggcaggtaac cgctggttaa acgttcatag cgcccttcat cttttagcgc ctgaatagaa
     7741 aggggaccaa aaacctcttc cggcgtggag aagcgagtca tcagatattc aaacgcgcgg
     7801 gcattctgaa aggcgaattt taagcgccgg gcgatcaaac ttttggcaat acctggcggg
     7861 ccaaggagga acacactttc accacttaat gccgctaata aacacaagcg gatggcgtgg
     7921 ctacgttcat aaagcccctt ttccaacgaa ctgctcaggc gggaaattct ttccgctaat
     7981 aaatgagggt gagccataat gaagtggcgt cctttcgtca aaagttctgc gtaaattgcg
     8041 agtatagacg tttcttgctg gtggctaaaa tagtctcaaa gggggggtat ttttctttga
     8101 gccaggttaa tgtgcccgca tttaggagta cgattttgcc gttaatcgtg catactgtgc
     8161 gcttttttgt gggccaaggg actaagcaca catttcatat ttcaacgaaa gactagtcta
     8221 tgagcactga taataagcaa tcattgcccg cgattaccct cgcggcgatt ggagttgtct
     8281 acggcgatat tggtaccagc ccgttatata cacttcgtga atgtttgtcc ggccagtttg
     8341 gttttggcgt tgagcgcgat gccgtgtttg gctttttatc gctgatcttc tggctgctaa
     8401 tctttgtggt ttctattaaa tatctcacct tcgtgatgcg ggcagataac gccggtgaag
     8461 gggggatcct gacgttgatg tcgcttgccg ggcgtaatac gtcggcgcga accacatcaa
     8521 tgctggtgat tatggggcta atcggcggca gctttttcta tggtgaagtc gtcataacac
     8581 ccgctatttc ggtgatgtcc gccattgaag gtctggaaat cgtcgccccg cagctggata
     8641 cctggatagt tcccctctca attatcgttc tcactttatt atttatgatt caaaagcatg
     8701 gcacggctat ggtcggtaag ctgtttgcgc cgatcatgct gacctggttt ttgattctgg
     8761 caggactggg gttacgtagc attattgcta acccggaagt gctgcatgcg ctgaatccga
     8821 tgtgggcggt gcatttcttc cttgaataca aaacggtttc ttttattgca ttaggggcag
     8881 tggtgctgtc gattacgggg gtcgaggcgc tgtatgctga tatggggcac tttggtaagt
     8941 tccctattcg cctggcgtgg ttcaccgtcg tattaccttc cttgaccctt aattacttcg
     9001 gtcagggagc gttgttgtta aagaacccgg aagcgattaa gaacccgttc ttcctgttgg
     9061 caccggactg ggcgctgatc ccgctgctga tcatcgccgc actggcgacg gtaattgcct
     9121 cgcaggctgt tatctctggc gtcttctcat tgacgcgtca ggcggtacgt ctgggatatt
     9181 tgtcgccgat gcgcattatt cacacctccg aaatggagtc agggcaaatc tatattccct
     9241 ttgtgaactg gatgctctat gtcgcggtcg tgattgtgat tgtcagcttt gagcactcca
     9301 gcaacctggc ggcggcgtac gggattgcgg tgaccggaac catggtgctg acgtctattc
     9361 tctcgactac cgtggcacgt cagaactggc actggaataa gtattttgtt gcgctgatcc
     9421 tgattgcttt cctttgtgtc gatattccat tgttcaccgc taacctcgat aaactgctct
     9481 ccggcggctg gttgccattg agcctcggta cggtgatgtt tatcgtgatg accacctgga
     9541 agagcgaacg tttccgtttg ctgcggcgga tgcatgaaca tggtaactct ctggaggcga
     9601 tgattgcttc gctggagaaa tcaccgcccg ttcgcgtgcc cgggaccgcg gtgtatatgt
     9661 cacgtgcaat caacgtcatt ccctttgcgc tgatgcataa ccttaaacat aacaaggtat
     9721 tgcatgagcg ggtgattctg ttaactctgc gcaccgaaga cgcgccatat gtccataacg
     9781 tccgtcgggt acagattgaa caactgtcgc ccactttctg gcgcgtggtg gcaagttatg
     9841 gttggcgaga aacgccaaac gtggaagaag ttttccaccg ctgcggtctg gaaggattaa
     9901 gttgccggat gatggaaacc tccttcttta tgtcgcatga gtcgttgatc ctcggcaaac
     9961 gcccgtggta tttgcgtctg cgcggcaagc tgtacttgct gctgcaacgt aatgcgctgc
    10021 gtgcgccgga tcaatttgaa atcccgccaa acagggttat cgaactgggt actcaggtcg
    10081 aaatctaacg ccagacgcct cctttctcct taagggggcg tttctgtttt catggttaat
    10141 taccatgtaa aacgtttcga ggttgatcac atttccgtaa cgtcacgatg gttttcccaa
    10201 ctcagtcagg attaaactgc gggtcagcga aacgtttcgc tgatggagaa aaaaatgaaa
    10261 aaaggcaccg ttcttaattc tgatatttca tcggtgatct cccgtctggg acataccgat
    10321 acgctggtgg tgtgtgatgc tggtttaccc atccccaaaa gtacaacgcg tatcgatatg
    10381 gcattaaccc agggcgtacc ttcttttatg caggtgctgg gcgtcgtcac aaatgaaatg
    10441 caggtcgagg cagccattat cgcggaagag atcaaacaac ataatccgca tctccacgaa
    10501 acgttgctca ctcaccttga gcagctgcaa aaacaccagg gaaataccat tgaaattcgt
    10561 tacaccacgc atgaacaatt caaacaacaa accgcagaaa gtcaggcggt aattcgcagc
    10621 ggagaatgtt ctccgtatgc gaatatcatt ctctgtgctg gcgtgacgtt ctgaggccgt
    10681 catggaagca ttacttcagc ttaaaggcat cgataaagcc ttcccgggcg taaaagccct
    10741 ctcgggcgca gcgttaaatg tctatcctgg ccgcgtgatg gcgctggtgg gcgaaaacgg
    10801 tgcgggtaaa tccaccatga tgaaagtgct tacgggtatc tatactcgcg atgccggtac
    10861 gcttttatgg ctggggaaag aaacgacgtt taccgggccg aaatcttccc aggaagccgg
    10921 gattgggatt atccatcagg aactgaacct gatcccgcag ttgaccattg ccgaaaacat
    10981 tttcctcggt cgtgagtttg ttaatcgctt tggcaaaatt gactggaaaa ccatgtatgc
    11041 cgaagcggat aaattgctgg ctaaacttaa cctgcgcttt aaaagcgaca agctggtggg
    11101 cgatctttcc atcggtgacc agcaaatggt tgaaattgcc aaagtgctga gctttgagtc
    11161 gaaagtcatc attatggatg aaccgaccga tgcgctgacc gataccgaaa ccgaatcctt
    11221 gttccgcgtc atccgcgagc tgaaatcgca aggccgcggt attgtctata tctcccaccg
    11281 catgaaagaa atcttcgaga tttgcgatga cgttaccgtt tttcgtgatg ggcaatttat
    11341 tgctgagcgt gaagtagcat cactgaccga agattcgctg attgagatga tggtgggtcg
    11401 caagctggaa gatcaatatc cgcacctgga caaagcgccg ggagatatcc gcctgaaagt
    11461 cgataatctc tgcggacctg gcgttaacga tgtctctttt actttacgca aaggcgaaat
    11521 tcttggcgtc tctggtttga tgggcgcagg tcgtaccgaa ctgatgaaag tgctctacgg
    11581 cgcactgccg cgcaccagcg gttacgtcac cctggatggg catgaagtcg ttacccgttc
    11641 accgcaggat ggcctggcaa acggcattgt gtatatctcc gaagaccgta aacgtgacgg
    11701 tttagtgctg ggcatgtcag taaaagagaa catgtcgctg acagcgctgc gctacttcag
    11761 ccgcgctggc ggcagtttga agcatgccga tgaacagcag gcagtaagtg atttcattcg
    11821 tctgtttaat gtgaaaacgc cgtcgatgga acaggcaatt ggtctgctct ccggtggcaa
    11881 tcagcaaaaa gtggcgattg cccgtggtct gatgacacgc cccaaagtgt tgatcctcga
    11941 tgagcctacc cgtggcgtag atgtcggcgc gaaaaaagag atctatcaac tgattaacca
    12001 gttcaaagcc gatggcttga gcatcattct ggtgtcatcg gagatgccag aagtattagg
    12061 catgagcgat cgcatcatcg tcatgcatga agggcatctc agcggggaat ttactcgtga
    12121 gcaggccacc caggaagtgt taatggctgc cgctgtgggc aagcttaatc gcgtgaatca
    12181 ggagtaaaaa aatgacaacc cagactgtct ctggtcgccg ttatttcacg aaagcgtggc
    12241 tgatggagca gaaatcgctt atcgctctgc tggtgctgat cgcgattgtc tcgacgttaa
    12301 gcccgaactt tttcaccatc aataacttat tcaatattct ccagcaaacc tcggtgaacg
    12361 ccattatggc agtcgggatg acgctggtga tcctgacgtc gggcatcgac ttgtcggtag
    12421 gttctctatt ggcgctgacc ggcgcagttg ctgcatctat cgttggcatt gaagtcaatg
    12481 cgctggtggc tgtcgctgcc gctctcgcgt taggtgccgc aattggtgcg gtaaccgggg
    12541 tgattgtagc gaaaggtcgc gtccaggcct ttatcgctac gctggttatg atgcttttac
    12601 tgcgcggcgt gaccatggtt tataccaacg gtagcccagt gaatactggc tttactgaga
    12661 acgccgatct gtttggctgg tttggtattg gtcgtccgct gggtgtaccg acgccagtct
    12721 ggatcatggg aattgtcttc ctcgcggcct ggtacatgct gcatcacacg cgtctggggc
    12781 gttacatcta cgcgctgggc ggcaacgaag cggcaacgcg cctttctggt atcaacgtca
    12841 ataaaatcaa aatcatcgtc tattctcttt gtggtctgct ggcatcgctg gccgggatca
    12901 ttgaagtggc gcgtctctcc tccgcacaac ctacggcggg gactggctat gagctggatg
    12961 ccattgctgc ggtggttctg ggcggtacga gtctggcggg cggaaaaggt cgcattgttg
    13021 ggacgttgat cggcgcatta attcttggct tccttaataa tggattgaat ttgttaggtg
    13081 tttcctccta ttaccagatg atcgtcaaag cggtggtgat tttgctggcg gtgctggtag
    13141 acaacaaaaa gcagtaataa cgactacagg acatcttgaa tatgaacatg aaaaaactgg
    13201 ctaccctggt ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg atggcaaaag
    13261 acaccatcgc gctggtggtc tccacgctta acaacccgtt ctttgtatcg ctgaaagatg
    13321 gcgcgcagaa agaggcggat aaacttggct ataacctggt ggtgctggac tcccagaata
    13381 acccggcgaa agagctggcg aacgtgcagg acttaaccgt tcgcggcacc aaaattctgc
    13441 tgattaaccc gaccgactcc gacgcagtgg gtaatgctgt gaagatggct aaccaggcga
    13501 acatcccggt tatcactctt gaccgccagg caacgaaagg tgaagtggtg agccacattg
    13561 cttctgataa cgtactgggc ggcaaaatcg ccggtgatta catcgcgaag aaagcgggtg
    13621 aaggtgccaa agttatcgag ctgcaaggca ttgccggtac atccgcagcc cgtgaacgtg
    13681 gcgaaggctt ccagcaggct gttgctgctc acaagtttaa tgttcttgcc agccagccag
    13741 cagattttga tcgcactaaa ggtttgaacg taatgcagaa cctgttgacc gcacatccgg
    13801 atgttcaggc tgtattcgcg cagaatgatg aaatggcgct gggcgcgctg cgcgcactgc
    13861 aaactgccgg taaatcggat gtgatggtcg tcggatttga cggtacaccg gatggcgaaa
    13921 aagcggtgaa tgatggcaaa ctagcagcga ctatcgctca gctacccgat cagattggcg
    13981 cgaaaggcgt cgaaaccgcc gataaagtac tgaaaggtga gaaagttcag gctaagtatc
    14041 cggttgatct gaaactggtt gttaagcagt agttttaatc aggttgtatg acctgatggt
    14101 gacataaata cggcatcgac agatgaacgt gtaatataaa gaaaagcagg gcacgcgcca
    14161 ccctaacacg gtggcgcatt ttatggacat cccgaatatg caaaacgcag gcagcctcgt
    14221 tgttcttggc agcattaatg ctgaccacat tcttaatctt caatcttttc ctaccccagg
    14281 cgaaaccgta accggtaacc actatcaggt tgcatttggc ggcaaaggcg ctaatcaggc
    14341 tgtggctgct gggcgtagcg gtgcgaatat cgcgtttatt gcctgcactg gcgatgacag
    14401 cattggtgag agcgttcgcc agcagctcgc cactgataac atcgatattt ctccggtcag
    14461 cgtgatcaaa ggcgaatcaa caggtgtggc gctgattttt gttaatggcg aaggtgagaa
    14521 tgtcatcggt attcatgccg gcgctaatgc tgccctttcc ccggcactgg tggaagcgca
    14581 acgtgagcgt attgccaacg cgtcagcatt attaatgcag ctggaatcac cactcgaaag
    14641 tgtgatggca gcggcgaaaa tcgcccatca aaataagact atcgttgcgc ttaacccggc
    14701 tccggctcgc gaacttcctg acgaactgct ggcactggtg gacattatta cgccaaacga
    14761 aacggaagca gaaaagctca ccggtattcg tgttgaaaat gatgaagatg cagcgaaggc
    14821 ggcgcaggta ctgcatgaaa aaggtatccg tactgtactg attactttag gaagtcgtgg
    14881 tgtatgggct agcgtgaatg gtgaaggtca gcgcgttcct ggattccggg tgcaggctgt
    14941 cgataccatt gctgccggag atacctttaa cggcgcgtta atcacggcgt tgctggaaga
    15001 aaaaccattg ccagaggcga ttcggtttgc tcatgctgcc gctgcgattg ccgtaacacg
    15061 taaaggcgca caaccttccg taccgtggcg tgaagagatc gacgcatttt tagacaggca
    15121 gaggtgacgc ttggctacaa tgaaagatgt tgcccgcctg gcgggcgttt ctacctcaac
    15181 agtttctcac gttatcaata aagatcgctt cgtcagtgaa gcgattaccg ccaaagttga
    15241 agcggcgatt aaagaactca attacgcgcc atcagccctg gcgcgtagcc tcaaactcaa
    15301 tcaaacacat accattggca tgttgatcac tgccagtacc aatcctttct attctgaact
    15361 ggtgcgtggc gttgaacgca gttgcttcga acgcggttat agtcttgtcc tttgcaatac
    15421 cgaaggcgat gaacagcgga tgaatcgcaa tctggaaacg ctgatgcaaa aacgcgttga
    15481 tggcttgctg ttactgtgca ccgaaacgca tcaaccttcg cgtgaaatca tgcaacgtta
    15541 tccgacagtg cctactgtga tgatggactg ggctccgttc gatggcgaca gcgatcttat
    15601 tcaggataac tcgttgctgg gcggagactt agcaacgcaa tatctgatcg ataaaggtca
    15661 tacccgtatc gcctgtatta ccggcccgct ggataaaact ccggcacgcc tgcggttgga
    15721 aggttatcgg gcggcgatga aacgtgcggg tctcaacatt cctgatggct atgaagtcac
    15781 tggtgatttt gaatttaacg gcgggtttga cgctatgcgc caactgttat tacatccgct
    15841 gcgtcctcag gccgtcttta ccggaaatga cgctatggct gttggcgttt accaggcgct
    15901 gtatcaggca gagttacaag ttccgcagga tattgcggtg attggctatg acgatatcga
    15961 actggcaagc tttatgacgc caccattaac cactatccac caaccgaaag atgaactagg
    16021 ggagctggcg attgatgtac tcatccatcg gataacccaa ccgacccttc agcaacaacg
    16081 attacaactt actccgattc tgatggaacg cggttcggct tagatttacg ctgtcttttg
    16141 atcaaattat taccatcggt tgttttcaga agcatgaaca ttgctgctga agcaacagta
    16201 ataatgccca tcgtgataaa cgtatagtgg aattgttcga cagtcgttgt gccttccatc
    16261 ccttcataaa cgcgaaggac ggccgcactt acagcaacgc ctaaactgat agacagttgc
    16321 tgcgtgaccg ccagaacact gttaccgctg ctggcgttgt catcggtcag atcggcaagt
    16381 gtgatggtat tcatcgcggt aaattgcgtc gacatagcca tccctaatat aaacaacggc
    16441 aagatcagca tccatatcgc cattgccggt gattgcaaag agaactgagc gatcattagc
    16501 ccaataatca ccgtgatccc cactaatgta tggcgatagc ccagacgacg taagacttgg
    16561 gtaaccatcg attttgcaat aatggaacct aacgctgtcg gtgccatcat acagccggca
    16621 ataaacgcct gataaccaaa tcctacctgt aacatcaatg gcataaggaa cggtacacaa
    16681 ccggtcccca gacgggttgc aatattgcct acgataccga tcgagaaagt gcgggtttta
    16741 aataaatcta atgaaattaa tgggtttggc gtgcgtcgtg catggagaat atagagaagc
    16801 agtaacccga tgctggtgac aattaccgtc aaggcaatcc aactggcgac aatcttttcc
    16861 ccgaatagct ctattccgct tgagaagaga acaaggctaa ggccaaacag caaaaagcca
    16921 gtgatatcga atctgcgtcg tgcggtggtg aaattgggca tatgtttgcg cgcgtaaaga
    16981 aggcccgcaa tacctatggg gatattgatt aaaaatatcc agtgccaggt tgcccaggtg
    17041 accagcacgc cgccaagaac agggcctaaa attggcccca ccagacccgg catggcgaca
    17101 aaattcaata caggaagaag ctcattacga ggataagcgc gcagtaaggc cagccgagca
    17161 acaggcatca tcattgcgcc gcctatcccc tgaataaccc ggaagacaac cagctgtggt
    17221 agcgagttag aaagtgcgca ggccagagaa cccaacgtga acagactcac ggcaagagta
    17281 aatatgcgac gcgtaccgaa gcgatcggct agccatccgc ttaccggaat aagcatcgcc
    17341 accgtcagcg tataactgat gatggctgat tgcatcgcga gaggagaacg attaaggcta
    17401 tgagcgattg cgggtaaggc ggtattcaga atagtggcat caagtgcctg catgaagaag
    17461 gccatcgccg cgatccacgg caaacccgcc atactgcgct tctttttatc gctcattcaa
    17521 tgtcctgtta tcggggtgtc acttatcagg tgagcgtagc agcgcctgac aagctttaaa
    17581 tgccgcgtcg ccatcgcttt ggataatcgc gtcaacaatc gcctggtgca gatccagctt
    17641 tatcactgtg tcgctggtaa ttgacgtgaa gtaagtgtga taaaccgaat ggaagagcga
    17701 ggcgaatgag gtcaaaaacg gattggcgct catttcatag atatgctcat gccaggccat
    17761 atcgacctca atccagcgtt cacggcgaaa gttctctttt aatgccacca tttcggtcat
    17821 taacgtattg agatgcgcct tctgttccgc ggtgccaacc attgctgcca gtaggcaagc
    17881 ttgcggctcc agacagatac gcataaccag aaagtgatcg atgacctgat gaaagttctc
    17941 ttctgtcatc caccaggtaa gcaattcctg atcaagaaaa ttccagtttg attgtggcat
    18001 gacccgggta ccaattcgcg gtcgcggtaa aaccatccct tttgccgtta acgttttgac
    18061 cgcttcgcgt accgctgtac gactcactcc aaattgctcg cccagctcaa tctcaccagg
    18121 caaaatggtg ccgggttcat attcaccttt taagatgcgt tgcgccagct tctcagccag
    18181 aacatacgaa aggtttttct gtgcagctaa ctgttgtgcg cttaaaggca ttactaatct
    18241 tcctttttct ttttattcct ccttagtatg ccaccaggaa gtgtgattac ggttgcaaaa
    18301 acggcaaatt gcttgtttta tggcacatta acggggcttt tgctgaaaaa atgcgcggtc
    18361 agaaaattat tttaaatttc c
//
LOCUS       DAZUCC010000092        18261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZUCC010000092 DAZUCC010000000
VERSION     DAZUCC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.092"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            91..1311
                     /locus_tag="ACHY09_002687"
     CDS             91..1311
                     /locus_tag="ACHY09_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFS3885642.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDMPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1284..1913
                     /locus_tag="ACHY09_002688"
     CDS             1284..1913
                     /locus_tag="ACHY09_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFS3885643.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLIPNDYNPNNVAPPEKKLLKKSIETDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1914..3074)
                     /gene="ybdL"
                     /locus_tag="ACHY09_002689"
     CDS             complement(1914..3074)
                     /gene="ybdL"
                     /locus_tag="ACHY09_002689"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFS3885644.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VKFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3183..4271
                     /gene="hcxA"
                     /locus_tag="ACHY09_002690"
     CDS             3183..4271
                     /gene="hcxA"
                     /locus_tag="ACHY09_002690"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFS3885645.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTEAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFENVESFKA"
     gene            complement(4281..4478)
                     /locus_tag="ACHY09_002691"
     CDS             complement(4281..4478)
                     /locus_tag="ACHY09_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFS3885646.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4591..6696)
                     /gene="cstA"
                     /locus_tag="ACHY09_002692"
     CDS             complement(4591..6696)
                     /gene="cstA"
                     /locus_tag="ACHY09_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFS3885647.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALVALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6877..7290)
                     /gene="entH"
                     /locus_tag="ACHY09_002693"
     CDS             complement(6877..7290)
                     /gene="entH"
                     /locus_tag="ACHY09_002693"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFS3885648.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7293..8039)
                     /gene="entA"
                     /locus_tag="ACHY09_002694"
     CDS             complement(7293..8039)
                     /gene="entA"
                     /locus_tag="ACHY09_002694"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFS3885649.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8039..8896)
                     /gene="entB"
                     /locus_tag="ACHY09_002695"
     CDS             complement(8039..8896)
                     /gene="entB"
                     /locus_tag="ACHY09_002695"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFS3885650.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDSLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8910..10520)
                     /gene="entE"
                     /locus_tag="ACHY09_002696"
     CDS             complement(8910..10520)
                     /gene="entE"
                     /locus_tag="ACHY09_002696"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFS3885651.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10530..11705)
                     /gene="entC"
                     /locus_tag="ACHY09_002697"
     CDS             complement(10530..11705)
                     /gene="entC"
                     /locus_tag="ACHY09_002697"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFS3885652.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFAGAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11894..12850
                     /gene="fepB"
                     /locus_tag="ACHY09_002698"
     CDS             11894..12850
                     /gene="fepB"
                     /locus_tag="ACHY09_002698"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS3885653.1"
                     /translation="MRLVPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAETVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLASGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12854..14104)
                     /gene="entS"
                     /locus_tag="ACHY09_002699"
     CDS             complement(12854..14104)
                     /gene="entS"
                     /locus_tag="ACHY09_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFS3885654.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QIMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14215..15219
                     /gene="fepD"
                     /locus_tag="ACHY09_002700"
     CDS             14215..15219
                     /gene="fepD"
                     /locus_tag="ACHY09_002700"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFS3885655.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLYTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15216..16208
                     /gene="fepG"
                     /locus_tag="ACHY09_002701"
     CDS             15216..16208
                     /gene="fepG"
                     /locus_tag="ACHY09_002701"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFS3885656.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAITLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAASLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQLLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16205..17020
                     /gene="fepC"
                     /locus_tag="ACHY09_002702"
     CDS             16205..17020
                     /gene="fepC"
                     /locus_tag="ACHY09_002702"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFS3885657.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(17017..18150)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHY09_002703"
     CDS             complement(17017..18150)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHY09_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFS3885658.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEVDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GPGKAIIVILSALIGGIVACGGVLLRHAMASRKQDAIMADHLV"
BASE COUNT         4314 a         5162 c         4700 g         4085 t
ORIGIN      
        1 aaaataatgt gctctatctt gcttcgttta tcctataatt agcggattca tctcacgagt
       61 caccccgtaa cgttcagaga gcgtttttag atgtctattt ataaaatccc gcttccactg
      121 aatattctgg aagctgcaaa agaacgtatt acctggacac ttaataccct accccgtatc
      181 tgtgtttctt tttcgggtgg taaagattcc ggattaatgc tgcatctgac agccgaaatt
      241 gcccgacaaa tgggcaaaaa aatctgcgtt ttgtttatcg actgggaagc gcagttctca
      301 tgcactatta actatgttca gtcattacgt gagttttacg ccgatgtcat cgaagagttt
      361 tactgggttg cgctcccgct tacgacgcaa aattcccttt ctcaatacca acccgaatgg
      421 cagtgctggg aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc gataaccgat
      481 cctgactttt tctcctttta ccagcctggc atgaccttcg aacaatttgt gcgtgagttt
      541 gctgaatggt tttcacaaaa acgtccggcg gcgatgatga tcggcatccg tgcagatgag
      601 tcctacaacc gttttgtcgc catcgccagt ttaaataaac aacgttttgc cgatgatatg
      661 ccctggacca ccgccgcacc aggcggtcat agctggtaca tttaccccat ctacgactgg
      721 aaagtggctg atatctggac ctggtatgct aaccatcaac agctctgtaa cccactgtat
      781 aatataatgt atcaggcagg cgttcctctg cgtcatatgc gaatttgcga accttttggc
      841 ccggagcaac ggcaaggatt atggctctat cacgttatcg aaccggatcg ctgggctgct
      901 atgtgcgcac gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca tgataatcat
      961 ttttatggtc atcggaaaat cctcaagcca gaacatttag actggcaaga atatgcatta
     1021 ttgctgctca atagcatgcc ggaaaaaaca gctgagcatt accgcaataa aatcgccatt
     1081 tatttgcact ggtatcagaa aaaaggcatc gaagttccac aaacccagca aggggacatt
     1141 ggcgcgaaag atatcccctc ctggcggcga atatgcaaag ttttactcaa taacgattac
     1201 tggtgccggg cattatcatt tagtcctacg aaagcgaaga actatcagcg ttataacgaa
     1261 cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta acgcaggatt
     1321 taacccaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt aatgaaatcc
     1381 gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc gttctgtggg
     1441 ttaaaaacag ccaacttata cctaatgatt acaacccaaa caacgtggca ccacccgaaa
     1501 aaaaactgct gaaaaaatcc attgaaacag atggttttac ccaacccatt gtggtcacgc
     1561 atacggataa aaatgccctg gaaatcgtgg atggttttca ccgccatgaa attggtaaag
     1621 gctcatcatc gttaaaatta cgcttaaaag gttatttgcc ggtgacctgt ctggaaggta
     1681 cgcgtaatca acgcatcgcc gcgactattc gtcataaccg cgctcgtggt cgccaccaaa
     1741 tcactgccat gtccgaaatt gttcgtgaac tcagccagtt gggatgggac gataataaaa
     1801 ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaaacaa attaacggcc
     1861 tgcaagagtt gtttgccgac cgtcaatatt cccgtgcctg gactgtcaaa taactacagc
     1921 tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa acagagacga
     1981 atcagtttat gggggaaggg atcggcgcaa aataccgaca gcgggatcgc cgctacaccg
     2041 tgctcccgcg tcagccactg gcaaaactta acgtcatcta gcgaagaaac cgcgctgtaa
     2101 tccaccagca aaaagtatgt accttcgcac ggtaaaatct ccagccgact ctcacttaag
     2161 gcattcacca gaatatcgcg tttctggcga taaaagtccg gtaacgcaag ataatgctca
     2221 ggttctgcac gtagcatatc ggcaagcgcc aactgtgccg gggtattcac cgaaaaggtc
     2281 agatactgat gcactttgcg gatctcggcg ctgatgggcg caggcgcaac gcaataaccc
     2341 actttccagc cggtcatatg ataggtcttg ccaaacgatg acaccgccac cgctcgctca
     2401 cgcaattgcg gatgcgccag cacgctggca tggccctgtt gtgaaaaatt gatgtgctcg
     2461 tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca caaagcggcg
     2521 aaatcagcct gctgccagac agttgcactg gggttatgcg gggtgttgag gatcaccagt
     2581 cgggtgcgct cgcttaacaa tgcggcaaat tcctgccagt ccacgcgaaa atgcggtggt
     2641 tgcagtgcaa tacgtttcac tattcccccc gaaagcgcga tggcgggcgc gtaactgtca
     2701 tagctgggat caaagcagat cacttcatca ccattgcgca ccaatgcggt aatcgctgca
     2761 tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc cggttgatag
     2821 ccatacagac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac gccggtcatg
     2881 ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg taaatagcgc
     2941 ggaccatcaa aatcaggaaa gccttgtgaa aggttaatcg cctggtgttg ctgcgccagc
     3001 gcgctcatcc gggtgaaaat agtggtgcca agttgtggaa gtttgctttg tggaatcaga
     3061 gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca ctataacacg
     3121 atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa ggacaacaca
     3181 acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt tcacatcccg
     3241 gaagtttcaa tcacctgcac gattttttca ctgatgaaca actttctcgc gcggtgtgga
     3301 tctacggcga acgcgccatt gctgcggcac aaaccaaact gccgcctgcg tttgaactgc
     3361 caggggtaaa gcatattttg tttcgtggtc attgcagcga aagcgatgtg caacaactgg
     3421 cggcggagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc gcactactcg
     3481 acaccgcgaa agccctcgcc cgccgtctcg gtctgccgtt tgttgccgtt ccgacgatcg
     3541 ccgccacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc ggacaggcgc
     3601 tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggagcca gagattatcc
     3661 tcaatgcgcc gcaagaatat ctgctggcgg ggatcggtga cacgctggcg aaatggtatg
     3721 aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcgg ctggggatca
     3781 ataatgcgca ggccattcgc gacgtcttgt taaacagtag cgaacaggcg ctgagcgatc
     3841 agcaaaaaca acagttaacg caatcatttt gcgatgtggt ggatgctatt attgctggtg
     3901 gtgggatggt cggtggtctg ggcgatcgtt ttacgcgtgt ggcggcggcc catgccgtgc
     3961 ataacggtct gaccgttttg ccgcaaaccg agaagtttct ccacggcacc aaagtcgcct
     4021 acggaattct ggtgcaaagc gccttgctgg gtcaggatga tgtgctggcg caattaacag
     4081 aggcgtatca gcgttttcat ctgccgacta cgctggcgga gctggaagtg gatatcaata
     4141 atcaggtgga gatcgacaaa atgattgccc acaccctacg tccggtggaa tccattcatt
     4201 acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaac gtggaatcgt
     4261 ttaaagcctg acgtacaaat ttagcagcag cgcgcgccgc cttttccacc gtagcgcgcg
     4321 tcctgccgct cccggaaaaa ctcctcgtag gtcatcggtg tttgatcggg atggttaacc
     4381 cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagttt cgccgcctga
     4441 cctaaatatt ttccggcttt tgccagtgaa tcaaacatct aaacctccgg taatacaaat
     4501 gccctcctga attaagaggg cttaattgct atcaacacgc tcgggatgag agtaagggga
     4561 acgctcagca cattccccct cactctgact ttagtgcgcg cctttcgcct gcgccacgat
     4621 ctcctcgaca ttttccggca tcggctcata tggcgtttct ttcgccgttg gcttcggctc
     4681 tttcaatgcc accagtgccg tcttaatcga gaacagcgcc agaaccacca caaccaccat
     4741 aaagaagatg gttaatccgg catccagacg gttgttgaac accagttgcg ccagttgtga
     4801 ctcagtatac tgcgacggaa tattgccgct gtcgatcatt gcctggaact tgttagcaat
     4861 cgccaggaag ccgactttcg catccgggct aaacgctttc tgccagcctg cggtcagggt
     4921 acaaatcagc agccaggccg ttggtaccag cgccacccag gcgtaacgtt gacgtttcat
     4981 tttgaacagc accacagcac agagcatcag cgccatccct gccagcatct ggttggcaat
     5041 accaaacagc ggccacaagg tgttaatacc gcccagcgga tcgaccacgc cctgatgcag
     5101 gaagtagccc cacgccagca cgcacagcgc cgttgccagc aggttagcag gcagtgaatc
     5161 ggtccgtttc aggcccggag acaccacgcc cagcagatcc tgcaacataa agcgcgcagc
     5221 acgcgtaccc gcatccaccg ccgtcagaat aaacagtgct tcaaacagaa tggcgaagtg
     5281 ataccagaac gccacatcca tcatgccgcc cagcgcaccg tgcagaatgt aggccatccc
     5341 caccgccagg gtcggcgcac cgcccgcacg agaaatgatc gactgttcac ccacttcgct
     5401 ggcaatctgg tttagcgtat ccggggtaat cgcaaagccc cagctactca ccacctgcgc
     5461 ggcagaagca accacatccg ccgtccctgc cggagccagc accgccatcg ggctgttcat
     5521 ggcgaagtac acacccggat cgatgataca ggcggaaacc agcgccataa tcgccacgaa
     5581 ggattccatc aacatcccac cgtagccgat aaagcacgcc tgcccttcgt tcgccagcat
     5641 cttcggtgta gtgccggaag agatcagtgc atggaagcca gacaccgcgc cacaggcgat
     5701 ggtgataaac aggaacggga acaggttacc ggtccatacc gggccagtgc catcgacaaa
     5761 tttagttagc gcaggcatgg tcagcgtcgg gcgcataatc aaaatgccca ccgccagacc
     5821 gacgatagtc ccgattttca ggaaggttga gaggtagtca cgcggagcca gcagcaacca
     5881 caccggcagc accgccgcca caaaaccgta acccaccagc atccaggtca gctgcacgcc
     5941 ggtaaagtca aagtacggtg cccacgtcgg gctttctgcc acccagccgc cagagataat
     6001 ggcgaaaata aggaacacca gaccaatcac cgacacttcg ccaatacgcc ccggacgcaa
     6061 atagcgcagg tagatcccca taaacagcgc cagcggaatg gtgaacgcaa cggtatacgt
     6121 tccccacggg ctatgagtca gggctttcac cacgatcatc gccagcacgg caaggataat
     6181 gaccatgatc ataaagcagg ccaccagcgc aatcaccccg gcggttggcc ccatctcttc
     6241 tttgaccaac tcacccagcg agcgaccatc gcggcgcgta gaaacaaaca gcaccatgaa
     6301 atcctgcacc gcaccggcga gaaccacccc agcgagcagc cagatcatcc ctggcaggta
     6361 acccatttgc gccgccagta ccggcccaac caacggacct gctccggcaa tggccgcaaa
     6421 atggtgaccg aacagcactt ttttgtccgt cggcacatag tccagcccgt cgttatggcg
     6481 caccgctggc gtcatacgcg tcgggtcaac cgccagcaca tttttggcga tatacagccc
     6541 ataaaaacgg taagcgatca gatagataca gaccgacgcc accacaatcc acagcgcgtt
     6601 gatctgttcc ccacgattta aagcaatgta tcccagagca aatgctccca ttacagagag
     6661 cactgtccag acgaggtatt tccctgattt gttcatagtt gttatccgtg tgcgtgtcca
     6721 tagagatgtt acattttgtt tctataacat ctttgtacaa tttaacaaca cggcaaccat
     6781 gtaaatcgga gttgaaacca atatttaact taacaatgtt aactcgatca ctccgttaac
     6841 cgagttttcc tgcaatctca tttaattctg tcgggctcat cccaaaactg ccgtacccag
     6901 ccgacaagtg cagcaacgcc gcccctgttc atcgaaaaca acaatttccc agctctgatt
     6961 ttgccgacca agatgcagcg gctggcagac gccgcgtacc tttccctcag acaccgggcg
     7021 atgatgtgtg gcgttaagtt ccgtgcccac cacacactgt ccgtcgcgag tcatcataaa
     7081 tccggccatc gatcccagcg tttccgccag cgccgccgac gcgccgccat gtagtaaacc
     7141 gaacggctga tgagtacggg tatcaaccgg catttcggct tccagcacat catcgcccag
     7201 gcgggtatac acaattccca gatgcgccac cattgtgtta tcgctggtgg cgttcagctc
     7261 gtcgagcgtt aaatggcgtt tccagatcat gcttatgccc ccagcgtaga gccgccatct
     7321 accacaatat cctgtagggt gatatggctg gcgaggtcag aggcgaggaa caaaatcgtg
     7381 ttggcgatct cttgtggacg ggcgattttc cccagcggaa tgccaagttt aaactgctcg
     7441 ccaaagccgc gaatacgctg ctcttcggcg tcatcgctca cccacagcgt gcgttgcata
     7501 tcggtgtcgg tggagcctgg cgaaaccaca ttacagcgca cgccgctacc cgccagttcc
     7561 agcccaacgc tcaacgccag gcttttcagc gccgctttcg acgcgccata agcactcata
     7621 ccaatgcgcg gcgtgtgtgc ggcgtcggac gcaacagtaa caatcgcccc gccccgctga
     7681 cggcgaaact ggttcatggt ctgctggaac aggttaaacg caccgccgac attaaccgca
     7741 aaagtctgct gccagtcctc cttactgagc tgatcggtcg cgcccatgcg caaaattccc
     7801 gccgcattga ccagcacgtc cagtcgctcc gtttcagcta acagtcgctg acatacctgc
     7861 gccacctgcg cagcgtcggc aacatccatc acttcggtcg caaagggata ttgctcctga
     7921 gtgaacgctt gatcaaaacc tgtaactttc gctcccgcct caacaaacgc cagcgccgtg
     7981 gcgtagccga tacctttccc cgcgccagtt acccagacat ttttaccgct gaaatccatt
     8041 atttcacctc gcgggagagt agcttccacc aggcgtcgat ggtcgggttt ttcgccagca
     8101 tgacaaagtc gatatcgcca tgcactttgc gccagcgcgc cgccagcgcc atcatccgca
     8161 ccgaatccag accgtagtcg atcaggttgt cgtcatcgaa cggttcatcg gactcgtcca
     8221 gcaacggcag gatcacctca cgcagcgccg ctttgctggc ggggataggt gctggcagta
     8281 attcttcggt catcaccact cggccagaac gtccggccac atatttcagc gacatcaaat
     8341 gctcgtcacg gctgaaatcg gccagcgcat ctgccaccat aaacggttta atatcgcgca
     8401 taaatgcgtc ggttgcggtg gtcatacagc caatgtgggc atataccccg gtaataatca
     8461 gctggttacg tccactctct ttcagcattt gctccagcgg ggaacgatga aacgcgctgt
     8521 agcgccactt caccagcacc gtgtcgtcgg catctggcgt caggctatcc accacctttt
     8581 gctgttccgg cgaacgggtc aggccaggcc cccacatatc attcaacagc gcccgatctt
     8641 catcgctctg ctctttcggc tgggcggtgt aataaaccgg gatattgtgc tgtttgcagt
     8701 agtcgcgtag tgccgcaata ttcgcgatca cctgctccat catcgggcag ttctcgcccc
     8761 agaagctgac aaaatagtcc tgcatatcat ggattaacaa cgcggcacgt tgcggttcaa
     8821 aggcccagtc gactttattc tgcggaatat cgtgagactc cggcagtgcg taagcctgta
     8881 attttggaat agccatcgtg ttctctcctt caggctgatg cgcgtgacgc cagccactga
     8941 cgtaattgtt ttttatcgac tttcccgacc gccgtcagcg gaagtgaatc tacacactcc
     9001 acgcgatccg gtaatttaaa ttcggcaata ccctgttcac gcaggaaacg acgcacctgc
     9061 accgcgcgca gcggctcttt taccaccaga taagcacagc ttttttcgcc catcagctca
     9121 tcttccatgc tcaccagtgc ggcgtagatc accgccggat ggcgcagcag caggttttcg
     9181 atctcttcgg cagcgatctt ctcgccgcca cggttgatct gatctttctc gcgcccctgc
     9241 acagtgatgt aaccctctgg atcaatagag atcagatcgc cggaacagta aaaaccgttg
     9301 gcatcaaagg cgctggcatt gtgctgcggg cttttgtaat agccacggaa ggtatacgga
     9361 ccgcgggtca tcaggcgtcc aacttcccct tgcggcagtg gatttccttc ggcatcggca
     9421 acccacactt catcgtccgg acacattggg taaccctggg tatggataat tttctccgcg
     9481 ctatcatcaa gtcgggtgta gttcactagt ccttccgcca tgccgaatac ctgctgtaac
     9541 tggcagccaa tctctgcggg aatacgcgcc gcaagcgtgg cagaaagacg tgcaccaccg
     9601 acctgtaaca gtttcagcga ggcaagctgc gcccggcttt cgccttcagc cagtgcctgc
     9661 aaccacaggc tgactgcggg cggcaccagc gcggtaacgt tgacctgatg tttttcaatc
     9721 aatgggaagc aaagcgtggc gctggggtcg gcagccagaa cgacagtgcc gccagcgaga
     9781 aataccccca acgatcccgg cgaactcatg gcgtagttat gagccgccgg gatcgcgcac
     9841 aggtagcgcg tctgttgtgt gaactgacaa atctcgacgc tacgacgcac gctgtagtag
     9901 tagtcgttat gagtgcgcgg tatcagtttc ggagtgccgg tggtgccgcc ggaaagctgg
     9961 aaataggcca cttcatcagc aggagatggc gtggcagtaa aatcctcagc cggatggtta
    10021 atcgcatcct gcaagttatg ctcaccgctg tcgttgagca gctgcaccac gcgaatggaa
    10081 gaatgctctg caacaaatgt gttgaggaaa tcatccccgc taaatagcgc atgttgacga
    10141 tcggcaatca gtaacgccgg ttcaatctgg ctggcatagg cgttcagttc actacgctga
    10201 tggctgaaca gcgccagcac cggcgcaacg cccagcttca gcagcgcgaa aaaggtaata
    10261 tacaattcag cgacgttacc cagttgtacc agcgcggttt caccaggttt aatgccctga
    10321 cggcgcaaac tacaggcgag gttatccgcc gcctgattca gctcccggta actcaactgc
    10381 cgctcgccgt cgataaccgc gatgctgtca ctcgcagcgt ggcgagtcag aatgtcggtc
    10441 agtggcaaat cctgccagta gcctttttcc cgatagcgac gggcaaactc ttccggccag
    10501 cgggtgaatg gaatgctcat cctcgctcct taatgcaatc caaaaacgtt caacatggta
    10561 gaaagtttga cgcctgtttc gcgccactca cccaacggtg aggacgcagg cacaatcccc
    10621 gctccggcaa acagacgcac ctgattttcc cgcagcttcg cgcagcggat ggtcaccacc
    10681 cattcaccgt taccttcgct gtcacaccaa cccacaatgc cgccaaacag ttcgcggtcg
    10741 aacggctcca gttcagcaat aacctgagtc gcggcctgat gcgggaaacc gctcagcgcg
    10801 ggggtcggat gcagcagaca ggccagagtc agtgcgtttt cttgcgaatt cgctttacct
    10861 tcaaagggag ttgcgagatg ccacagcgtc ggcgtggtaa tcaattgtgg agaggaggga
    10921 acgtgtaact cactactgcg tttgcgcagt acctctttca tcgcctgagt caccagttca
    10981 tgttcatggc gatctttttc tgacgccagc agacgattac ccgcttcgcg atcgagcact
    11041 tcatccggct gacgacgcgc ggaaccggct aacggaatgg agctaaaacg ctcgccgtct
    11101 ttacgtagca gcagttccgg gctggccccc agcaggacgc cgccatcagc cagcgggaca
    11161 tggaagttgt aactaaccgg gttttgcgca atcaaccgtt ccagcaatac gccactatca
    11221 atggcggcgt cagtggtgat atcaatcaac cgtgacaaca ccactttgtc gacctgcggc
    11281 gtggcggtaa gtgcggcggc gcgggcaacc atctgttcaa acgtggtttg ttccggaatt
    11341 gcctggcgtt ccaccacatt cagcgactgg ctgcgggtga aacggcgtgc ggaagcttgt
    11401 ttttcctgac gggagaacga ctgccaggat tcgggaatat acagcgatga aggctgacgt
    11461 ggatcgaagg gaatcgcccc gaccatcacc ggatttttga tgccctgcgc tttggcaccg
    11521 gcaaacagcg cggcgagttt ttgctggaag ggactgtcgg gcgaatcccc gttcacagcc
    11581 ggttcatcga agcgggcgaa acatcctgac gtcgtaaaac tgcggtacgg cgacataaag
    11641 aaaaagcgat tgggcgcaag tgttgccatg gtctgctgta cttcctcagc cagtgacgta
    11701 tccatatcat cctccacaaa atgataaagg ctttaataat gattatcatt tatattttcg
    11761 gtcgctaacc taaaagcaaa cgcgcactat gtcaactctt gaggtaacgc aatctgcaac
    11821 tcggggttgc atctggttgt ggttacaatg aaaatgagaa gcattaactt cattaataac
    11881 aggaagctga tttgtgagac tcgtcccgct ctaccgcaac gcccttctat taacaggact
    11941 tttgctttca ggaatagccg tagttcaggc cgccgactgg ccgcgtcaga ttactgacag
    12001 ccggggcact cataccctgg aaagccagcc gcagcgtatt gtttccacca gcgtcaccct
    12061 gactggctca ctgctggcga ttgatgctcc ggtgatcgcc agcggcgcga ccacgccgaa
    12121 taaccgcgtc gcggatgacc agggcttttt acgccagtgg agcaaggtgg cgaaagaacg
    12181 caaactgcaa cggctctata tcggcgagcc gagtgcagaa accgttgccg cgcaaatgcc
    12241 ggatctgatt ttaattagcg caaccggcgg tgattcagcg ctggcgctgt acgatcagct
    12301 ttccaccatc gccccaacat taatcatcaa ttacgacgac aaaagctggc agtcgctgtt
    12361 aacgcagctt ggcgaaatta ccgggcatga gaaacaagcg gcagagcgga ttgcacagtt
    12421 tgataagcaa ctggcggcgg cgaaagagca aatcaaatta ccgccgcagc cggtcactgc
    12481 cattgtctat accgccgctg cacacagtgc caatctctgg acgccggaat cagcacaagg
    12541 gcagatgctg gaacaactcg gctttacgtt ggcgaaattg cccgcagggt taaacgccag
    12601 ccaaagccag ggcaaacgcc atgacatcat tcagcttggt ggggaaaatc tggcttcagg
    12661 gttaaatggc gagtcactat tcctgttcgc cggtgatcag aaagatgccg atgcgattta
    12721 tgctaatccg ctgctcgcgc acctgcctgc agtacaaaac aagcaggttt atgcgctggg
    12781 aaccgagacg ttccgtctgg attactacag cgccatgcaa gtgctggaac ggcttaaggc
    12841 gctgttttaa gcattaactg tcggacgctg tcacctgcgg cggcgtctgg cgaaaacgcc
    12901 gcaactccac cagcaccagc aataacaaca cgccgataat caacaaacca aaaccgctcg
    12961 cgcttgcgga agcaaccggt gtcatcatcg cgcccaaacc gcccagcagc gccgcgccta
    13021 tggcatcgcc cgtcacgttt tgcgccgtcc acaaaccgtt aatccgccct aacatcgctt
    13081 ccggggtttg cgtttgcagc attgtgtatt gcagcaacga actaaccgcg ctcagccagc
    13141 cgaacagcgc cagacaaacc acgcctaaaa tccacatcgg catcaggcca aacagaccaa
    13201 tggcgaggaa cgatcccagc gtggaaagca gcatcaataa ccctggtcgc gcgctatgtg
    13261 ccagcttccc gctggttaac gcgccaatag ccgcaccgag cgggatcgcc gcatagagaa
    13321 aaccaatctg tgccgccgac atctgccagt tgtcagccag cgccggatac aggacccgca
    13381 ccgcgctcgc catcgtcaat aaaccaccca acagcgcaat ccctcctacc agcgggctgg
    13441 cgagcagaaa acgaaatcct gccagtaatg atttcaacgg atgctcacgc ggctgcggtg
    13501 gtggtggtaa cgccggaagg cttaacaacg gtagcaaggt aataaacgtg cccgccgccg
    13561 ccagcccgta gttccaggcc acaccgccag tcgccagcag taaaccgcca atcatgggcg
    13621 aaatcaccga tcccagacgc acggtcaaca tggtgatcgc cccggcctgc attaagtttt
    13681 cacgccctac cagtgctggt gtcgccgcca gtagcgccgt aacgccaagt gatgcgaaaa
    13741 aaccatccca taaaccaagt aaatagattg ccagcaatga cggctccggc agcagtgcat
    13801 taaggcacag tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa atcacttttt
    13861 tgcgctcata gcgatccgcc agcacaccgc cgaccatcag gccaacaaac atcgcgccgc
    13921 cggtcagcgt caccgaaagc cccacctgcc aggtagaatg cgtcattatc tggatctgca
    13981 ccggtaccgc gacgccgagc aaacccagag acacaattga gataaaacga gcgaggaata
    14041 ctgcgcgaaa cgccgggtgc gttttcaaca ggctgaggtt aagcagccag gattgtttat
    14101 tcattacaat gccttgccat caaatatttt aaatacctat tcttaggctg cacatgctaa
    14161 catatccaaa taagatcgat aacgataatt aatttcatta tcatggaagt tcgtatgtct
    14221 ggttctgttg ccgtgacacg cgccattgcc gtgcccggat tgctgttatt actgattatc
    14281 gcgacggcat taagcctgct cattggggca aaatcactcc ccgcttccgt agtgctggag
    14341 gccctctccg gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc gcggctgccg
    14401 cgcacccttg ccggtttact ggcaggcggc gcgcttgggc ttgccggggc gttaatgcaa
    14461 accctcaccc gaaacccact tgccgacccc ggcttgcttg gcgtaaacgc cggagccagc
    14521 tttgccattg tgctgggtgc ggcgctgttt ggttactcat ccgcgcagga acaactggcg
    14581 atggccttcg ccggggcgct ggtggcctcg ttgattgttg cctttaccgg cagccagggc
    14641 ggcgggcagt taagtccggt gcgtttaacc ctggcgggcg tggcgctggc ggcggtgctg
    14701 gaagggctga ccagcggcat cgccctgctt aatcctgacg tctacgatca actgcgtttc
    14761 tggcaagccg gttcgctgga tattcgcaat ctatatacct taaaagttgt gctgatcccg
    14821 gtgctgatcg ccggagcaac cgcgctatta ctgagccgcg cgctgaacag tttaagcctc
    14881 ggcagtgaca ccgcgacagc gctgggcagc cgcgtggcgc gcacacagtt gattggtctg
    14941 ctggcgatta ccgtgctttg tggtagtgcg acggcggtag ttggcccgat tgcctttatt
    15001 ggcctgatga tgccgcatat ggcgcgttgg ttggttggag cagatcatcg ctggtcgctg
    15061 cccgtcacgc tacttgccac ccctgccctg ctgctgtttg ccgatatcat cgggcgggtg
    15121 attgtgcccg gcgaactgcg agtttccgtg gtcagtgcgt ttattggtgc accggtgctg
    15181 atcttcctcg tgcgacgtaa aacgcgaggt ggcgcatgat ttacgtctct cgccgattaa
    15241 ttatcacctg tttgctgctg gtttccgcct gtgtggttgc aggtatctgg ggattacgca
    15301 gcggtgccat cacgctggaa acctcgcagg tattcgccgc gctgatgggc gatgcgccgc
    15361 gcagtatgac gatggtggtc accgaatggc gtttaccacg cgtgctgatg gcgctgttga
    15421 ttggcgcggc gctgggcgtc agcggcgcga tttttcagtc gctgatgcgt aacccgctcg
    15481 gcagccctga cgtaatgggc tttaacaccg gggcatggag cggcgtactg gtggcgatgg
    15541 tgctgtttgg tcaggatctg acggctatcg cgctggcagc aatggcgggg ggtattgtca
    15601 cctcgctgtt ggtctggctg ctcgcctggc gtaacggtat cgacaccttt cggttgatta
    15661 ttatcggtat cggtgttcgc gccatgctgg tggcctttaa tacctggctg ttgctgaaag
    15721 cgtctttaga aacggcgcta acagcgggtt tgtggaatgc cggatcgctc aacggcctga
    15781 cgtgggcaaa aacctcacct tccgcgccaa tcattatatt gatgctcatt gccgcctcct
    15841 tgctggtgcg gcgaatgcgc ttgctggaaa tgggcgatga taccgcatgt gcgctgggcg
    15901 tcagcgtcga acgttcgcgg ctgttaatga tgctggtcgc cgtagtgctt accgctgcgg
    15961 caacagcgct tgccgggccg atttccttta ttgctttagt cgcgccgcac attgcccgcc
    16021 gcattagtgg caccgctcgc tgggggctaa cccaggcggc gctgtgcggg gcgttgttac
    16081 tgctggtggc cgatctctgc gcccaactac tgtttatgcc gtatcaactt ccggttggcg
    16141 tcgttaccgt cagcctcggt ggtatttacc ttatcgtctt gttaattcag gagtctcgca
    16201 aaaaatgacc gaatcagtag cccgtttgcg cggcgaacag ttaaccctcg gatatggcaa
    16261 atataccgtt gcggaaaatc tgactgtaga aatacctgat ggtcacttca cggcaattat
    16321 cgggccaaat ggctgcggta aatccacgtt gctgcgtacc ttaagccgcc tgatgacgcc
    16381 tgctcacggg catgtctggc tggatggcga gcacattcaa cattacgcca gtaaagaggt
    16441 tgcacgccgg attggcctgt tggcgcaaaa cgccaccacg ccgggagata tcaccgtgca
    16501 ggagctggtg gcgcgtgggc gttacccgca tcaaccgctg tttacccgct ggcgcaaaga
    16561 agatgaagac gcggtaacga aagcgatgca ggccacggga atcactcatc tggcagatca
    16621 aagcgtggat accctttctg gcggacaacg ccagcgagcg tggatagcaa tggtgctggc
    16681 ccaggaaacg gcaattatgc tgctcgatga acccacgacc tggctggata tcagtcatca
    16741 gattgatttg ctggaactgt taagcgaact gaaccgcgag aaaggctata ccctggcggc
    16801 ggtgctgcac gatcttaatc aggcctgtcg ttacgccagc catttgattg cattgcggga
    16861 agggaaaatt gttgctcagg gagcgccgaa ggagattgtc actgctgaac tgatcgagcg
    16921 catttatggt ctgcgctgca tgattattga cgatccggtt gccggaacgc cgctcgtggt
    16981 gccgctcgga cgaacgacac cttcaaccgc gaagatttaa actaagtggt ctgccattat
    17041 ggcatcctgt tttctggatg ccatcgcatg gcgcaatagc acgccaccac aagccactat
    17101 accgccgatc aacgcagaaa ggatcacaat aatcgcctta cccggaccgt ctttttttac
    17161 gggtaatgac ggacgcaact gatatttaaa cggtgtaaaa ttcacatcgt tgacatttgt
    17221 ttttgttaat tgctcgacta gatactgacg attacgtaat tcaccgttca attccgcaac
    17281 gtcagtgact gctttttcaa tttccagttt gcgttcaata ccgtctgcgc cgagagaaat
    17341 agagaaatcg gggtcatctt taacggcctg accattactg tatacgggct ttttaattcc
    17401 tgccgcgttg gcaatgtcga gtgaataatt gaggcgctga atgtttgcat caagttgatt
    17461 tttcgtttta atgcgatcct gagccagttt ttctttttca aactgggttt tgatctccag
    17521 tttattacgg acgttttcta tcgactcttt caccaccaac gcagagatat aatcgatata
    17581 cccggccaac actttctgtg cctcttcact ggtgggtgcg gtgaaactta gcgtccagga
    17641 agtatacaat gcagactcat cttttttctt actggcattg tcatcaaccg ctttcatttt
    17701 ttcgctcaat gcgacaattg cgcgatgcaa atccagttcg tcgattttcg cctcttttaa
    17761 ttggtccatc acataaggtg ttgagcgcag gtactcttcc agcaagctaa ccgactgaaa
    17821 cttcttgata aacaggttaa atgcttctgt acgatcaatt ttgatatcca gatccagcac
    17881 acgaagctta gtgaatgttt tctcaagcgc ctgccactga acaggttctg gaggcgtgac
    17941 aaccgccgcg ctggtccatt tttgcggcag gatgaaagag atcagtaaac ctgcgcaggc
    18001 aaacgcaaaa acgaccgcca tgaccgtttt tttggcccgc cataaaactt caattagatt
    18061 aagtaaatca acttcattat tactgggcga cgccagagga tactcgggaa aatgagcttc
    18121 acttccctgt ttaatattca gtgatgacat ggggtataac ctgaaaatga gtccaataag
    18181 agtaaatctc cacggccccc actctaccag ttggtgagat ttatccgaag ctgaagtttg
    18241 taaattactc ccactgaatg c
//
LOCUS       DAZUCC010000093        17980 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZUCC010000093 DAZUCC010000000
VERSION     DAZUCC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.093"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(243..1088)
                     /gene="sseA"
                     /locus_tag="ACHY09_002704"
     CDS             complement(243..1088)
                     /gene="sseA"
                     /locus_tag="ACHY09_002704"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFS3885659.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            1295..6256
                     /gene="yfhM"
                     /locus_tag="ACHY09_002705"
     CDS             1295..6256
                     /gene="yfhM"
                     /locus_tag="ACHY09_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFS3885660.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQEFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNSARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGVEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTTSGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFTLWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLSASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            6257..8569
                     /gene="pbpC"
                     /locus_tag="ACHY09_002706"
     CDS             6257..8569
                     /gene="pbpC"
                     /locus_tag="ACHY09_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFS3885661.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWMHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLALLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAIPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLAEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            8718..9149
                     /gene="ndk"
                     /locus_tag="ACHY09_002707"
     CDS             8718..9149
                     /gene="ndk"
                     /locus_tag="ACHY09_002707"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS3885662.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            9299..10453
                     /locus_tag="ACHY09_002708"
     CDS             9299..10453
                     /locus_tag="ACHY09_002708"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFS3885663.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            10738..11745
                     /gene="rodZ"
                     /locus_tag="ACHY09_002709"
     CDS             10738..11745
                     /gene="rodZ"
                     /locus_tag="ACHY09_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFS3885664.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            11772..12890
                     /gene="ispG"
                     /locus_tag="ACHY09_002710"
                     /gene_synonym="gcpE"
     CDS             11772..12890
                     /gene="ispG"
                     /locus_tag="ACHY09_002710"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFS3885665.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            13001..14275
                     /gene="hisS"
                     /locus_tag="ACHY09_002711"
     CDS             13001..14275
                     /gene="hisS"
                     /locus_tag="ACHY09_002711"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS3885666.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRTLGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            14293..14913
                     /gene="yfgM"
                     /locus_tag="ACHY09_002712"
     CDS             14293..14913
                     /gene="yfgM"
                     /locus_tag="ACHY09_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFS3885667.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            14924..16102
                     /gene="bamB"
                     /locus_tag="ACHY09_002713"
     CDS             14924..16102
                     /gene="bamB"
                     /locus_tag="ACHY09_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFS3885668.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            16220..17692
                     /gene="der"
                     /locus_tag="ACHY09_002714"
     CDS             16220..17692
                     /gene="der"
                     /locus_tag="ACHY09_002714"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS3885669.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEADDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            17755..17970
                     /locus_tag="ACHY09_002715"
     CDS             17755..17970
                     /locus_tag="ACHY09_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFS3885670.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRVEFIIA"
BASE COUNT         4309 a         4432 c         5037 g         4202 t
ORIGIN      
        1 gactaataag ccaggttatt ttattaccga acacggcgga aagggccggt gtcgataata
       61 cgctaaataa acaatgatta ctctctttcg cttgacaaaa agagagttac tggcgagtat
      121 tgctttgctg tgaagcacct gcgttgctca tgcaacacaa gccaatctgc tggaaaggcc
      181 gcgaaccaga ccagcaataa aaaaccgcca gattcggcgg ttttttattg cctgttaaaa
      241 gcttatttct ctggctcaac tggtaaatct gctcgtgcgc cccattcact ccatgcgccg
      301 tcgtacagtt tcacgtttgg cacatcaagt gtcgcgagtg ccaacaaaac cacggctgcc
      361 gttacaccag agccgcagct gacgataata ggtttgtcat aacttacgcc gcgaccaaaa
      421 aatatcgcat ccagttcatc ggtcgttttt agttcacctt cgcgcaccag ttccgtccac
      481 ggaacattta gtgcaccggg aatatgtccg cgacgtaaac ctgggcgagg ttcatcaact
      541 tctgcgttaa aacgtgcagc cgggcgggca tcgataattt gcgccgtatt ttcatggctt
      601 gccaataaca catcggttac tttcaccacg gcttcaggat taaacgcggc gttaaactct
      661 ccttccggca gctctactgc gccttcttct aatagcagat catcacgctg ccagcctgca
      721 agtccaccac ccagaatcga cactttctct acaccaaagg tgcgcagcat ccaccatgct
      781 cgtggggctg agaaaagatt accttcgtca tagacaatca ggtgcttatc ctggttaacg
      841 cctaattcac gcatcgccac ggcgaacgtt tccgggcgcg gcagcatgtg cggaagcggg
      901 gaagtgtgat cagaaagcgc ttctatatca aaaaacactg cgccgggaat atgtccattc
      961 agatactcct gagcaacgtt acgatcttcc tgtccaggcg acgccatgcg ggcatcgata
     1021 atctgaattt ccgggtcatc aatatgttcg gcgagccagt cggctcctac aaaccatgtc
     1081 gtggacatag gcatctccat ttttgccggg gttaggctaa attgtcggat gtttagtgaa
     1141 aggcgacaag caaaagaggc tgatttggct gtctgagcca caattctcac taaaattcgg
     1201 attgtatggg cgtgatactg aattacataa cgtaaattct cacgcataat aattattcga
     1261 cgttgctaac gggccattgt ggcagggatg aaaaatgaaa aagttacgcg tagccgcctg
     1321 catgctaatg ctggcgctgg cagggtgcga caacaacgat aacgcgccaa cagcggtgaa
     1381 aaaagatgcg ccttctgaag ttactaaagc ggcctcttca gaaaacgtga gttcagcaaa
     1441 actttccgcg ccagagcgac aaaaactggc ccaacagagt gccggtaagg cgctgacatt
     1501 gctggatctc tctgaagttc aacttgatgg cgcagccacg ctggtgctga cattctccat
     1561 ccctctcgac ccggatcagg aattctcacg cgttatccat gtcgtcgata agaaaagcgg
     1621 caaagtggat ggtgcctggg agctgtcaga taatcttaaa gagttgcgtt tacgccacct
     1681 cgaaccgaaa cgtgatttga tcgtcactat tggcaaggag gtcaaagcac tcaacaacgc
     1741 aaccttcagt aaagattacg aaaaaactat aactacccga gatatccaac ccagcgtcgg
     1801 ttttgccagc cgtggctcgt tgttacctgg taaagttatt gaagggctgc cggtaatggc
     1861 gctcaacgtt aacaatgtcg atgttaactt cttccgtgtt aagccagaat ctctgcctgc
     1921 attcattagc caatgggaat accgcaattc gctggcgaac tggcagtctg acaaactgct
     1981 gcagatggcg gatctggttt ataccggacg gtttgatctc aattctgcgc gtaacacccg
     2041 tgaaaaatta ttgctgccgc tgggcgatat caaaccgctt cagcaggcag gcgtgtatct
     2101 ggctgtgatg aatcaggctg gacgttacga ttacagtaat cccgcgacgc tgtttacgtt
     2161 aagtgatatc ggcgtttcag ctcaccgtta tcacaatcgt ctggatatct ttacccaaag
     2221 cctggaaaac ggcgcggccc agcaaggagt tgaagtctca ttattaaatg agaaagggca
     2281 gactctgact caggcaacca gtgatgccca ggggcatgtg cagctggaaa atgataaaaa
     2341 cgcggcatta ctgttggcgc gtaaagacgg tcagacaacg ctactcgatt taaaacttcc
     2401 ggcgctggac ttagcagaat ttaacattgc tggcgcgcca ggctatagca aacagttttt
     2461 catgtttggc ccgcgcgatc tttatcgccc tggtgaaaca gtcatcctca atggtttatt
     2521 gcgtgatgca gatggtaaag cattgcccga tcaacccatc aagttagacg tgattaaacc
     2581 cgacgggcag gtactcagga gcgtcgttag tcagccggag aatggcctct accactttac
     2641 ctggccactc gatagcaatg cggcaaccgg tatgtggcat attcgcgcta acacgggcga
     2701 taaccagtat cggatgtggg atttccacgt cgaagatttt atgccagagc gcatggcgct
     2761 gaatctgacc ggtgagaaaa ccccgctaac gccgaaagat gaagtgagat tctccgtggt
     2821 ggggtactac ctgtatggtg cgcctgctaa tggtaatact ttgcaagggc aacttttcct
     2881 gcgcccactg cgtgaagctg tgtcagcctt acctggtttc gaattcggcg atatagctgc
     2941 cgaaaatctt tcccgcacgc tggatgaagt tcagttgacg ctggatgata aagggcgtgg
     3001 cgaagtttct acagaaagcc agtggaagga aacgcattcc ccattacagg ttattttcca
     3061 gggtagtttg ttggaatcgg gcggtcgccc ggtgacgcgc cgcgctgagc aggctatctg
     3121 gcctgccgat gcattgccgg ggatccgtcc gcagttcgcc tcgaaatcgg tttacgatta
     3181 tcgcactgac agcacggtga aacagcccat tgttgatgaa ggcagtaacg ccgcttttga
     3241 catcgtttat agcgatgcgc aaggcgtaaa aaaagccgtg tcgggcttgc aggtgcgcct
     3301 gattcgcgaa cgccgcgatt actactggaa ctggtcagaa gatgaaggct ggcagtcaca
     3361 gtttgatcaa aaagatctga tcgaaaatga acaaactctg gatctgaaag cggacgaaac
     3421 cggcaaggtc agtttcccgg tagagtgggg cgcttatcgt ctggaagtca aagcgccgaa
     3481 tgaagcggtc agtagtgtgc gtttctgggc tggctatagc tggcaggaca acagcgacgg
     3541 gagcggtgcc gtgcgacccg accgtgtcac gctgaaactg gataaagcca gctatcgccc
     3601 tggcgatacc attaagttgc atattgccgc gccaacggcg ggtaaaggtt atgcgatggt
     3661 cgagtccagt gaagggccgc tgtggtggca agagattgat gttccggctc aagggctgga
     3721 tctgacgatt ccggtcgata aaacctggaa tcgtcatgat ctgtatttaa gtacgctggt
     3781 ggtacgtcct ggcgataaat ctcgctccgc gacgccaaaa cgcgcggttg gggtgttgca
     3841 tctgccgctt ggtgatgaaa accgtcgcct cgatctggcg ctggaaaccc ctaccaaaat
     3901 gcgccccaat cagccattaa ccgtgaaaat taaagccagc aataaaaatg gtgagatgcc
     3961 aaaacaggtg aatgtgctgg tgtctgccgt tgatagcggt gtgctgaata ttactgatta
     4021 cgtcacgcca gatccgtggc aggcgttctt tggtcagaaa cgctatggcg cagacattta
     4081 cgatatttac ggtcaggtca ttgaaggtca ggggcgtctg gcagctctgc gttttggtgg
     4141 tgatggtgat gagctgaaac gtggtggtaa accgccggtc aatcacgtca atattgtcgc
     4201 gcagcaggcg ctgccggtaa cgctcaacga acagggcgaa ggctcggtta cactgccaat
     4261 tggcgatttt aacggtgaat tgcgcgtcat ggcgcaagcc tggacggcag atgacttcgg
     4321 aagcaacgaa agcaaagtga ttgttgctgc accggtgatt gctgaactga acatgccacg
     4381 ctttatggcg agtggcgata cctcgcgcct gacgctggat atcactaatc ttaccgataa
     4441 accgcaaaaa cttaatgttg ccctgaccgc cagtggtttg cttgaactgg tcagcgattc
     4501 acccgcagcc gttgaattag cgccaggggt gcgtactacg ctgtttatcc cggtgcgtgc
     4561 attgccgggt tatggcgatg gagacattca ggccaccatt agcgggttag cgttacccgg
     4621 ggaaaccgtt gccgatcagc ataagcagtg gaaaatcggc gtccgtccgg cattcccggc
     4681 acaaacggtc aattacggta cggcgttaca gcctggtgaa acctgggcgc tcccggctga
     4741 tggattgcaa aacttctcgc ctgttacgct ggaagggcaa ttgttgttga gcggcaaacc
     4801 accgctgaac atcgcacgtt atatcaaaga gttaaaagcg tatccgtacg gctgtctcga
     4861 gcaaaccacc agcggcctgt tcccgtcact ttataccaat gcagcccaac tgcaggcgtt
     4921 gggcatcaaa ggcgacagtg atgagaaacg ccgtgcatcg gtcgatatcg gcatttcccg
     4981 tttgctgcaa atgcaacgtg ataacggcgg ctttacgctg tgggataaaa acggtgacga
     5041 agagtactgg ctgacggctt acgtgatgga tttcctggtc cgcgcaggcg aacagggtta
     5101 cagcgtaccg acagacgcca ttaaccgggg taatgagcgt ctgctgcgct atttacaaga
     5161 tccgggcatg atgtcgatcc cgtacgctga taatctcaaa gccagtaaat tcgccgtaca
     5221 gtcttacgct gcgctggtgt tggcccgtca gcaaaaagca ccactgggtg cgctgcgtga
     5281 aatctgggag catcgtgcag atgccgcttc tggtttaccg ctgctgcaac ttggcgttgc
     5341 gctgaaaacc atgggtgatg caatgcgtgg tgaagaagcg attgtgctgg cgctgaaaac
     5401 gccgcgtaat agtgatgagc ggatatggct gggtgattac ggtagtccac tgcgcgacag
     5461 cgcgttgatg ctctcattgc tggaagagaa caaactgcta cccgatgagc agtactccct
     5521 gttgaacaca ctttcgcagc aggcgttcgg tgaacgctgg ctatcgacgc aggaaagtaa
     5581 cgcgttgttc ctggctgccc gtactcttca ggatttaccg ggcaaatggc aggcgcaaac
     5641 cactttctct gctgagccgc tgacaggcga gaaagcgcaa accagcaatc tgaatagcga
     5701 tcaacttgcc accttgcagg tgaccaacag tggcgatcag ccgttatggc tgcgtgtgga
     5761 tgccagcggt tatccgcaat ccgcaccttt atcggcgagc aatgtgctgc aaattgagcg
     5821 tcatattctg ggtactgacg gtaagagcaa atcgctggac tcgttacgta gcggcgatct
     5881 ggtgctggtg tggttgcagg ttaaagccag taacagtgta ccggatgcgt tagtcgtgga
     5941 tctgttgcct gcggggctgg aactggaaaa ccagaatctg gcgaacggca gcgccagcct
     6001 ggagcaaagt ggtggtgaag tgcagaactt gctgaaccag atgcagcagg cgagcattaa
     6061 gcacatagag ttccgtgacg atcgctttgt ggcggcggtt gccgttgatg aatatcaacc
     6121 ggtaacgctg gtgtatctgg cgcgggcggt gacgccggga acgtatcagg tgccgcagcc
     6181 tatggtggaa tcaatgtatg ttcctcaatg gcgggcgact ggtgcggctg acgatctgtt
     6241 gatagtcaga ccgtaaatgc ctcgcctgtt aaccaaacgc ggctgctgga taatgctggc
     6301 agccgcgcct tttatcatca ttctggcagc atgggcagca gataaactct ggcctctgcc
     6361 attgcaagaa gtgaatcctg cacgcgtggt ggtggcgcag gatggtacgc cactctggcg
     6421 tttcgccgat gctgacggta tctggcgtta tccggtaaca atcgaagatg tttctccacg
     6481 ttaccttgaa gcgctgatca attatgaaga tcgctggttc tggatgcatc cgggggtgaa
     6541 tccgttctcg gtggcgcgtg cagcctggca agatctcacg tcgggtcggg tcatttccgg
     6601 cggcagcacg ctcaccatgc aggtggcgcg cctgcttgat cctcacccca aaacgtttgg
     6661 cggcaaaatt cgccagctct ggcgcgcgtt gcaactggaa tggcatctgt ctaaacgtga
     6721 aattctgacc ttgtatctta accgtgcccc gtttggcggt acgttgcagg ggattggtgc
     6781 ggcaagttgg gcttacctcg ggaaatcgcc tgcgaattta agctattccg aggcggcaat
     6841 gctggcggtt ttgccgcaag cgcccagccg tcttcgcccg gatcgttggc cggagcgtgc
     6901 cgaagccgcg cgtaataaag tgctcgaacg gatggccgtg caaggtgtgt ggtcccgtga
     6961 gcgggtaaaa gagtcaaggg aagaacccat ctggctggca ccccgacaaa tgccgcaact
     7021 ggcactgctg ttttcgcgca tgatgctcgg taaaagcaaa agcgacaaaa tcgttactac
     7081 gctggatgcc ggtcttcaac gacgtctgga agaactggcg caaaactgga aaggacgatt
     7141 gccaccgcgc agctcactgg cgatgatcgt ggttgatcat accgatatgc gtgttcgcgg
     7201 ctgggtggga tcagttgatc tcaacgatga ttcacgcttt ggtcatgttg atatggtcaa
     7261 tgcgatccga tcgccaggat cggtgctcaa accgtttgtt tatggtctgg cgctggatga
     7321 aggcttgatc cacccggcat cgctgttgca agacgtcccc agacgcaccg gtgattaccg
     7381 acctggcaac tttgacagcg gttttcatgg cccgatcagc atgagtgagg cgctggtgcg
     7441 atcgctgaac ttacctgctg tgcaggtgct ggaagcctat ggaccgaaac ggtttgcggc
     7501 aaagttacgc aatgttggat tgccgttata tttgcccaac ggtgctgcac cgaatctttc
     7561 gctcattctc ggcggcgctg gcgcaaaact ggaagatatg gcggcagcgt ataccgcgtt
     7621 tgctcgccac ggcaaggcag gcaaattgcg cttacagcct gacgatccgc tgcttgaacg
     7681 gcctttaatg tcgtcggggg ctgcgtggat cattcgccgg attatggctg atgaagcgca
     7741 acccttgccg gatggtgcct tgccgcgcgt cgccccactg gcatggaaaa cgggcaccag
     7801 ctatggctat cgtgatgcct gggcgattgg ggttaacgct cgctatgtca ttgggatctg
     7861 gactggcaga ccggacggca cgcccgttgt tggtcagttt ggctttgcca gtgccatacc
     7921 gctgttgaat caggttaata atatcttact gtcgcgcagt gtgaatttgc cggaagaccc
     7981 acgtcctgac tctgtaagtc gtggcgttat ctgctggccg ggcggacagt ctttggcgga
     8041 aggtgacggt aactgtcgcc gccgcctggc aacctggctg ctggacggga gccagccgcc
     8101 aactctatta ctgccggagc aggaaggcat taatggcatt cgtttcccca tctggctgga
     8161 tgaaaacggc aaacgtgttg ccgctgattg ccctcaggcg cgacaagaaa tgataaatgt
     8221 ctggccgcta ccgctggaac cctggctgcc cgcatcagaa cgtcgcgctg tgcgcttgcc
     8281 accggcctcg acaatttgcc cgccatacgg tcacgatgca caactcccgc tgcagctaac
     8341 aggtgtgcgc gatggcgcga ttattaaacg tttaccgggc gcagcggaag cgactttgcc
     8401 gttgcaatca agtggagggg caggtgaacg ctggtggttt ctgaacggcg aaccgttaac
     8461 tgaacgtggg cgcaacgtga ccctgcattt gacggataaa ggcgattatc aattgctggt
     8521 gatggatgag gtggggcaaa tagcgacagt gaaatttgtc atgcaatagt ctgttttgtc
     8581 cctgattgtt gcttaaactg ggtttatttt aaaaaaatgt taccttcatc aatagtcaac
     8641 ggctctgttg ctcattataa tccgcgccat ctcgtacgct ggtacagaca acaacagaac
     8701 aatttacaga ggtaaaaatg gctattgaac gtactttttc catcatcaaa ccgaacgcgg
     8761 tagcaaaaaa cgtgattggt agtatctttg cgcgctttga agctgcaggg ttcaaaattg
     8821 tcggcaccaa aatgctgcac ctgaccgttg aacaggctcg tggcttttat gctgaacacg
     8881 atggaaaacc gttctttgat ggtctggttg aattcatgac ttctggcccg atcgttgttt
     8941 ccgtgctgga aggtgaaaat gctgttcagc gtcaccgcga tctgctgggc gcgaccaatc
     9001 cggcaaacgc actggccggt actctgcgtg ctgattacgc tgacagcctg accgaaaatg
     9061 gtacccacgg ttctgattcc gtcgaatctg ccgctcgcga aatcgcttat ttcttcggcg
     9121 aaggcgaagt gtgcccgcgc acccgttaat aatttcgtaa atgccgcgtg caaacgtggc
     9181 atccgtgcgc cagaatttgt acaatgcagc gcccccggac gagcagccgc tcaccggggc
     9241 gtttcttttt tcaaccctcc aggggccata acgtgtaata acgaggccgg aatagcatat
     9301 gtctgaacaa ttagtcacac ctgaaaacgt caccactaaa gatggaaaaa tcaacctgct
     9361 ggatctcaac cgtcagcaga tgcgggagtt ttttaaagat ttaggtgaaa aacccttccg
     9421 cgccgatcag gtgatgaagt ggatgtatca ctattgctgc gacaactttg atgagatgac
     9481 cgacatcaac aaagtgttgc gcggcaaact gaaagaggtg gcggagatcc gcgcaccgga
     9541 agtggttgaa gaacagcgtt catctgacgg caccattaaa tgggcaattg ccgttggcga
     9601 tcagcgcgtc gaaacggtgt atatcccgga agacgaccgt gccacgctct gcgtctcttc
     9661 gcaggtgggg tgtgcgctgg agtgtaaatt ctgttccacc gcccagcagg gctttaaccg
     9721 caacctgcgg gtatcggaaa ttatcggcca ggtgtggcgt gcggcgaaaa ttgtcggcgc
     9781 agcaaaagtc accggtcagc gcccaatcac taacgtcgtg atgatgggta tgggcgagcc
     9841 gctgctcaac ctgaacaacg tcgttccggc gatggaaatc atgcttgatg atttcggttt
     9901 tggcctgtca aaacgtcgcg tcacgctttc cacttccggt gtggttccgg cgctggataa
     9961 actgggcgat atgatcgacg ttgcgctggc tatctccctg cacgcgccga acgacgaaat
    10021 tcgtgacgaa attgtgccga tcaacaaaaa gtacaatatc gaaacgttcc ttgcggcggt
    10081 gcgccgttat ctggagaaat ccaacgccaa tcagggccga gtcactattg aatacgtgat
    10141 gcttgaccac gttaacgacg gcactgaaca cgcgcaccaa ctggcggaac tgctgaaaga
    10201 tacgccgtgt aagatcaacc tgatcccgtg gaacccgttc ccgggcgcgc cgtatggacg
    10261 cagttcgaac agccgtatcg accgtttctc aaaagtgctg atgagctacg gtttcaccac
    10321 tattgtgcgt aaaacgcgtg gtgatgacat tgatgctgcc tgtggtcagt tggcgggcga
    10381 tgttatcgac cgtacgaaac gtaccctgcg taaacgaatg cagggtgaag ccatcgacat
    10441 taaagcggtc tgatagccat tacgccacgg ttactttatg taccgtggcg taatctttta
    10501 tgaatgaagt attaagttgc aattgtctgg tcaatattaa cggtgcgttt ttgctgactt
    10561 taaggcagta tgtaacgatg caacagtaag ttagccttag cgaatgtggg ctttgtcacg
    10621 agcacacaga cggtcttata ctgtatgata acggttaact taacggatgt ttcgcggtgt
    10681 gggtgggcat gatggttcac cggcatctca attctcattt aaacgtacct gcagcgaatg
    10741 aatactgaag ccacgcacga ccaaaatgaa gcacttacta ccggcgctcg cctgcgtaat
    10801 gctcgcgaac aactaggact tagtcaacag gccgttgccg agcgactttg cctgaaggtt
    10861 tccacggtac gcgacattga agaagataag gcacccgccg atcttgcttc aacattcctg
    10921 cgcggatata tccgctctta tgcgcgtctg gtacatattc cagaagaaga actgctgcca
    10981 gggctggaaa agcaggctcc actacgggct gcaaaagttg cgccgatgca gagtttttcc
    11041 ctcggtaaac gccgcaaaaa acgtgacggc tggctgatga ccttcacctg gctggtattg
    11101 tttgtggtta ttggcctgag cggtgcctgg tggtggcaag accacaaagc tcagcaggaa
    11161 gagatcacca ctatggccga tcaatcttct gcggaactga ataataatca aagccagagt
    11221 gtaccgttag atacgtcgac aaccacagat caggctatgg cgaccacgcc gacatcacct
    11281 gtggatacca ccgcaaccaa cacgcaaaca cctgccgtaa ctgcgccagc accagctgtt
    11341 gatccgcaac agaatgcggt tgttccacct tcgcaggcaa atgttgatac cgctgcgact
    11401 ccggcaccag cggcaacaac aacaccagat ggcgctgcgc cgttgccaac cgatcaggct
    11461 ggcgtgacca cgccggcggt tgacccgaat gcgctggtga tgaactttac tgccgattgc
    11521 tggctggaag tcaccgatgc taccggtaaa aaactgttta gcggtatgca gcgtaaagac
    11581 ggtaatttga acttaaccgg ccaggcaccg tacaaactga aaattggtgc gccagccgca
    11641 gtacagatcc agtatcaagg gaaacctgtc gatctgagtc gttttatcag aactaaccag
    11701 gttgcgcgtc tgaccctcaa tgccgaacaa tcaccggcgc agtaacagac gggtaacgcg
    11761 ggagattttt catgcataac caggctccaa ttcaacgtag aaaatcaaca cgtatttacg
    11821 ttgggaatgt gccgattggc gatggtgctc ccatcgccgt acagtccatg accaatacgc
    11881 gcacgacaga cgtcgaagca acggtcaatc aaatcaaggc gctggaacgt gttggcgctg
    11941 atatcgtccg tgtctccgta ccgacgatgg acgcggcaga agcgttcaaa ctcatcaaac
    12001 agcaggttaa cgtgccgctg gtggctgaca tccacttcga ctatcgcatt gcgctgaaag
    12061 tagcggaata cggcgtcgat tgtctgcgta ttaaccctgg caatatcggt aatgaagagc
    12121 gtattcgcat ggtggttgac tgtgcgcgcg ataaaaacat tccgatccgt attggcgtta
    12181 atgccggatc gttggaaaaa gatctgcaag aaaagtatgg cgaaccgacg ccgcaggcgt
    12241 tgctggaatc tgccatgcgt catgttgatc atctcgatcg cctgaacttc gatcagttca
    12301 aagtcagcgt gaaagcgtct gacgtcttcc tcgctgttga gtcttatcgt ttgctggcaa
    12361 aacaaatcga tcagccgctg catctgggga tcaccgaagc cggtggtgcg cgtagcgggg
    12421 cagtaaaatc cgctattggt ttaggtctgc tgctgtctga aggcatcggc gacacgctgc
    12481 gcgtatcgct ggcggccgat ccggtcgaag agatcaaagt cggtttcgat attttgaaat
    12541 cgctgcgtat ccgttcgcgc gggatcaact tcatcgcctg cccgacatgt tcgcgtcagg
    12601 aatttgatgt tatcggtacg gttaacgcgc tggagcaacg cctggaagat atcatcactc
    12661 cgatggacgt ttcgattatc ggctgcgtgg tgaatggccc aggtgaggcg ctggtttcta
    12721 cactcggcgt cactggcggc aacaagaaaa gcggcctcta tgaagatggc gtgcgcaaag
    12781 accgtctgga caacaacgat atgatcgatc agctggaagc gcgcattcgt gcgaaagcca
    12841 gtcagctgga cgaagcgcgt cgaattgacg ttcagcaggt tgaaaaataa taacgtgatg
    12901 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
    12961 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
    13021 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
    13081 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
    13141 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
    13201 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
    13261 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
    13321 tatcgggcct atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
    13381 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgccgaac tgattatgct
    13441 cactgcccgc tggtggcgca cgctgggtat ttccgagcac gtaactcttg agctgaactc
    13501 tatcggttcg ctggaagctc gtgcgaatta ccgcgatgcg ctggtggcat tccttgagca
    13561 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cgttgcgcgt
    13621 actcgattca aaaaatccgg aagtgcaggt gcttctcaac gacgctccgg cattaggcga
    13681 ttatctggac gaggaatctc gtgagcactt tgccggtctg tgcaaactgc ttgagagcgc
    13741 gggtatcgct tacaccgtaa accagcgtct ggtgcgcggt ctggattact ataaccgtac
    13801 cgttttcgag tgggtgacta acagcctcgg ttcccagggc accgtgtgtg caggtggtcg
    13861 ttatgacggt ctggtggaac aactgggtgg tcgtgcaaca ccggcagtcg gttttgcgat
    13921 gggcctcgaa cgtcttgtat tgttagttca ggccgttaat ccggaattta aagccgatcc
    13981 tgttgtcgat atatacctgg tggcttcagg tgctgacaca caatctgcgg ctatggcatt
    14041 agcagagcgt ctgcgtgatg aattaccggg cgtgaaactg atgaccaacc acggcggcgg
    14101 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
    14161 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
    14221 aactgcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
    14281 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggttgaagc ggttaaacgc
    14341 ttttttgctg aaaatggcaa agcactggct gttggggtga ttttgggcgt tggcgcactg
    14401 attggctggc gctactggaa tagccatcag gttgattctg cacgctccgc ttctcttgcc
    14461 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc agcggcggaa
    14521 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
    14581 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
    14641 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
    14701 cagctcaagc aggctgatgc cgcgctaaaa acccttgatg ctatcaaagg tgaagggtgg
    14761 gctgccattg ttgccgacct gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
    14821 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ccccggcact gagcgaaatg
    14881 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
    14941 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
    15001 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgactac
    15061 tgcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
    15121 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttagtaaaag cgctgaatgc
    15181 ggatgacggc aaagaaatct ggtctgtcaa cctggccgag aaagatggct ggttctctaa
    15241 agatcctgca ttactttctg gcggcgtgac cgtgtctggt gggcatgtct acattggcag
    15301 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
    15361 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
    15421 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
    15481 cctcgatatg ccgtcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
    15541 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
    15601 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
    15661 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
    15721 cctgacggcg cttgatctgc gcagcggtca gattatgtgg aaacgcgaac tgggttcggt
    15781 gaatgatttc atcgtcgacg gcaaccgcat ctatctggtc gatcaaaatg accgggtgat
    15841 ggcgttgacc attgatggcg gcgttacgct gtggacacaa agcgatctgc tgcatcgcct
    15901 gctgacttct ccggtgctgt ataatggcaa cctggtggta ggtgacagtg aaggttatct
    15961 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
    16021 tttccagact gaaccggttg ctgctgacgg caaactgctg atccaggcaa aagacggaac
    16081 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
    16141 ggggccgttt tcctgttttt aacaacgaca cgaatatagc gtgcgttgtc tgatgattta
    16201 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
    16261 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
    16321 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgcg agtttatctg
    16381 cattgatacc ggcgggattg atggcaccga agacggtgta gagacacgta tggcggaaca
    16441 gtcgctgctg gcgattgaag aagcggacgt tgtactgttt atggtggatg cgcgcgcggg
    16501 cctgatgccg gcggatgaag cgattgctaa acatctgcgc tcccgtgaaa aaccgacctt
    16561 cctggtggca aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctacgc
    16621 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    16681 gctggagcac gtgctgctgc cgtggatgga agatctcgca ccgcaagaag aagtcgacga
    16741 agacgccgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggcaga
    16801 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    16861 taagtctaca ctcactaacc gtattcttgg tgaagagcgc gttgttgttt acgacatgcc
    16921 tggcacgacg cgtgacagta tctacattcc aatggaacgc gacggacgtg agtatgtgct
    16981 cattgacacc gctggtgtac gtaaacgcgg caaaatcacc gatgcggtag agaaattctc
    17041 ggtaatcaaa acgttgcagg ccattgaaga tgccaacgtg gtgatgttgg tgattgatgc
    17101 gcgtgaaggt atttccgatc aggatctctc cttgctgggc tttattctca atagtgggcg
    17161 ctcacttgtg attgtagtga acaagtggga cggcctgagc caggaagtga aagagcaggt
    17221 gaaagagacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    17281 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    17341 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    17401 tcaccaacca ccgctggtac gcggtcgtcg tgtgaagctg aaatacgccc acgccggggg
    17461 ttataacccg ccgattgtgg tgattcacgg caatcaggtg aaagacctgc ctgattccta
    17521 caagcgttac ctgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    17581 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    17641 ccagatgcgt aaacgtaagc gtctgatgaa gcatatcaag aaaagtaagt aatcacttat
    17701 ctggtttccc tttccacacg gagagggaag ccgaataaca cgcgaggagt atttatggca
    17761 ctcaactgtc caacctgcaa aacccctctc gaacgcaatg gcgagactgc tcactgtgca
    17821 acttgcgaca aagactttac cgtccaggca ctttgccctg agtgtcatca ggcgttacaa
    17881 gtgttaaaag cttgtggtgc tgtggattat ttctgccaac atgggcacgg tttaatatct
    17941 aaaaaacgcg ttgaatttat tattgcttaa tatcatgtga
//
LOCUS       DAZUCC010000094        17941 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZUCC010000094 DAZUCC010000000
VERSION     DAZUCC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.094"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..904)
                     /locus_tag="ACHY09_002716"
     CDS             complement(<1..904)
                     /locus_tag="ACHY09_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFS3885671.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLI"
     gene            complement(916..2130)
                     /gene="rspA"
                     /locus_tag="ACHY09_002717"
     CDS             complement(916..2130)
                     /gene="rspA"
                     /locus_tag="ACHY09_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFS3885672.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2336..2662)
                     /gene="ynfA"
                     /locus_tag="ACHY09_002718"
     CDS             complement(2336..2662)
                     /gene="ynfA"
                     /locus_tag="ACHY09_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFS3885673.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2797..3138
                     /gene="ynfB"
                     /locus_tag="ACHY09_002719"
     CDS             2797..3138
                     /gene="ynfB"
                     /locus_tag="ACHY09_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFS3885674.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3173..3733
                     /gene="speG"
                     /locus_tag="ACHY09_002720"
     CDS             3173..3733
                     /gene="speG"
                     /locus_tag="ACHY09_002720"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFS3885675.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3736..4446)
                     /gene="ynfC"
                     /locus_tag="ACHY09_002721"
     CDS             complement(3736..4446)
                     /gene="ynfC"
                     /locus_tag="ACHY09_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFS3885676.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4554..4859
                     /gene="ynfD"
                     /locus_tag="ACHY09_002722"
     CDS             4554..4859
                     /gene="ynfD"
                     /locus_tag="ACHY09_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFS3885677.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            5058..7484
                     /gene="ynfE"
                     /locus_tag="ACHY09_002723"
     CDS             5058..7484
                     /gene="ynfE"
                     /locus_tag="ACHY09_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFS3885678.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLVNENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            7545..9968
                     /gene="ynfF"
                     /locus_tag="ACHY09_002724"
     CDS             7545..9968
                     /gene="ynfF"
                     /locus_tag="ACHY09_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFS3885679.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            9979..10596
                     /gene="ynfG"
                     /locus_tag="ACHY09_002725"
     CDS             9979..10596
                     /gene="ynfG"
                     /locus_tag="ACHY09_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFS3885680.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            10598..11452
                     /gene="ynfH"
                     /locus_tag="ACHY09_002726"
     CDS             10598..11452
                     /gene="ynfH"
                     /locus_tag="ACHY09_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFS3885681.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAANNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWYNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            11495..12109
                     /gene="dmsD"
                     /locus_tag="ACHY09_002727"
     CDS             11495..12109
                     /gene="dmsD"
                     /locus_tag="ACHY09_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFS3885682.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            12268..13560
                     /gene="clcB"
                     /locus_tag="ACHY09_002728"
     CDS             12268..13560
                     /gene="clcB"
                     /locus_tag="ACHY09_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFS3885683.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(13513..14208)
                     /gene="bioD"
                     /locus_tag="ACHY09_002729"
     CDS             complement(13513..14208)
                     /gene="bioD"
                     /locus_tag="ACHY09_002729"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFS3885684.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(14333..15553)
                     /gene="mlc"
                     /locus_tag="ACHY09_002730"
                     /gene_synonym="dgsA"
     CDS             complement(14333..15553)
                     /gene="mlc"
                     /locus_tag="ACHY09_002730"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFS3885685.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(15688..16581)
                     /gene="ynfL"
                     /locus_tag="ACHY09_002731"
     CDS             complement(15688..16581)
                     /gene="ynfL"
                     /locus_tag="ACHY09_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3885686.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            16688..17941
                     /gene="ynfM"
                     /locus_tag="ACHY09_002732"
     CDS             16688..17941
                     /gene="ynfM"
                     /locus_tag="ACHY09_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3885687.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYVGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
BASE COUNT         4455 a         4635 c         4393 g         4458 t
ORIGIN      
        1 taattaaccc tttacttaac cagtcgataa cgaccggaaa tttatttgca tttaagcgtg
       61 aagagaaaat agagagttct tttccggtaa ttccttgctg aatcacttca gacggttcac
      121 tggagaagcc catcaataca atacgtgccg ctggagaagc cagcgttaca gcctctttca
      181 ggatagaagg atgacaagcc gcatcgataa ttaatgtcgg cttgatgcct ttttcagcga
      241 aactctcgcc aagcggtgtc tggctgttat taatcgccca gtctgccccg ctctctttcg
      301 ctttttccag tcgttcatca atgcgatcgg caacaatcac atttttaacg ttatagacgc
      361 cttttaatac ctgaacgatc gtcaggccga ttggaccggc accgtaaacc agaacggtat
      421 cattttcagt cggttgacca tgaccggtaa cgttagccgc aatggtaaaa ggttcgatca
      481 tcaccgcata ttgatcggcc actgcttcag gaattttcca cgcatttttt gccggaacca
      541 cggcatattc actgaaacca ccgtcagcgt gcacacctaa tacagccagt gtcgtacaaa
      601 cgttcggctt acctatagag cacggatagc aatgcccaca gctgaccacc ggatcgacag
      661 caacgcgttc accgactctg gcgctttcca cgccttcacc cactgcatca atgacgccaa
      721 agaattcatg accaatgacg cgcggatatt tcgcaaaagg attatgccca cgataaatat
      781 ggctatctga accacaaatt ccggcaagtt tcacttttac tcgtacttca cccgctgacg
      841 gggtgggtat ttcgcgttcg ataattgcca gttgattcgg tttttcaatt aaaatgcttt
      901 tcattatctt actccttacc agttccacag cgtgccatct tccagacgtg cgactggcag
      961 ataagcaggt tcatagggat atttcgccgc cagcttttca tcgaattcga tgccaagacc
     1021 cggtttgtct cccggatgca tatagccgtt atcgaaagtc cagttgtgcg ggaagacttc
     1081 aagcatttgt tcggaataac ccatgtactc ctggacaccg aaattgggga cccacagatc
     1141 aaagtgcagc gccgcagcca tgcagactgg cgacaaatcg gaaggaccgt gtgagccagt
     1201 acgtacctga tacagcgaag caaaatcggc aatccggcgc ataccggtaa tcccccccgc
     1261 gtgagtcagc gtggtgcgga tatagtcgat aagttgttct tcaatcagct gtttgcagtc
     1321 ccagatgctg ttgaagactt cacccactgc aattggcgtg acggtgtgtt ggcgaatcag
     1381 gcggaaacac tcctggtttt ccgcaggcgt cgggtcttcc atccagaaca tgcgataatc
     1441 ttcaatgctt ttaccaaagc gcgccgcttc aataggcgtt aagcgatggt gcatgtcatg
     1501 cagcaaatgt tcattaaaac caaacttgtt acgtaccgcg tcaaacaatt tcggcatgaa
     1561 atcgaggtat ttctccgtcg accacagctg ctcttccggc cactgtcctt tggttgcggg
     1621 ttcataagcc agacctttac ctttcgacat gccataggtg gttttcatac cagggattcc
     1681 gcactgcacg cggatggctt tgaatcccag ctcctgatga cgggcataat catccagagc
     1741 ttcatcaata ctgtgaccgg tggtatggca ataaaccatc accccttcac gagacgcgcc
     1801 gccgagtaac tggtaaagcg gcatgttggc agctttggct ttaatatccc acagcgccat
     1861 atcaaccgct gaaatggccg acatcgtaac cggaccgcga cgccagtaag cacctttata
     1921 gaaaaactgc cagatatctt cgatacggtg tgcatcgcga ccaataagct gcggacaaag
     1981 gtgatcctgc aaataagagg ccacggaaag ctcacgtcca ttgagggtgg catccccaag
     2041 gcccgtaata ccgtcctcag tggtgatttt taatgtgacg aaattacgcc ccggacaggt
     2101 aacaaaaact tcagccttta cgatcttcat gttcgattcc ttgcatcgct tgtcgtgatg
     2161 catgaaatct acgcaactga gctactacca tacaagtatg aagatcaaaa aaagccggag
     2221 tgatcacaaa aaaaggcgta tatttgcgcc gtgaatggtt gacaaaagat gaaatagaat
     2281 accttttgtc agctgacact tcctcgtatc ttattgataa aagggattta tgttcctacg
     2341 tgcgccccca gcccgcaaca atgatcaaca tgccgcaaag cgcaatcaac gcccccgtcc
     2401 agtcataaag actcagtttc acgccatcca caacgcgcag ccacatcaac gccgtgcaga
     2461 cataaacgcc accataagcg gcgtaaacac gcccactcgc cgcaggatgc aacgttaaca
     2521 accagacaaa cagcgccagt gaaatccccg ccggaagcaa cagccagata ctggcgtttc
     2581 gttttaacca caaccagggc agaaagcatc caataatttc acacagcgca gtagcaaaaa
     2641 atagtaacgt tgttttaatc atctttgtca cttattgaca tcatgtatag ttataggacg
     2701 acataatatc atcaatataa acatcctcct ggtacgtttt gcgttcgcag tggatggtgt
     2761 agaatcacct ttaatcattc atacagggaa tgaattatga aaattactct cagcaaacga
     2821 attggcctgc tcgcttttct gctgccttgc gcactggcat tgagcacaac tgttcatgcc
     2881 gaaactaaca aactggtgat tgagtctggc gacagtgcac aaagccgcca gcacgccgct
     2941 atggaaaaag agcaatggaa tgacacgcgc aatctgcgcc agaaagtgaa taaacgcact
     3001 gaaaaagagt gggataaagc cgacgccgct tttgataacc gcgataaatg tgagcaaagc
     3061 gccaacatca atgcctactg ggagccaaat actttgcgct gcctggaccg tcgaactggc
     3121 cgcgttatta ccccctaacc tgttattgat ttaaggaatg taaggacacg ttatgccaag
     3181 cgcccacagt gttaagctac gcccgctgga gcgtgaagat ttacgctatg tacatcaact
     3241 cgacaataac gccagtgtga tgcgttactg gtttgaggaa ccctacgaag cctttgttga
     3301 actctctgat ctgtacgata agcatattca cgatcagagc gaacggcgct ttgtggtgga
     3361 atgtgacggc gaaaaagccg gtctggtgga gctggtggaa attaaccatg ttcatcgccg
     3421 cgcagaattt cagataatta tctccccgga atatcagggg aaaggtctgg caacgcgtgc
     3481 cgcaaaatta gcaatggact atggcttcac cgttctcaat ctctataagc tgtatctgat
     3541 cgtcgataaa gagaatgaaa aagcgattca catttaccgc aagcttggct ttacggttga
     3601 aggtgaattg atgcatgagt tctttattaa tggtcaatat cgtaatgcca ttcgcatgtg
     3661 catattccag catcagtatc tggcagagca caaaacaccg ggtcagactc tcctgaagcc
     3721 gaccgcacaa tagcattaat aataatcgat agtatttttg atggtgtaaa cccgttcgac
     3781 ggcgggtttt actccttcat caacaatgat tagctgacag tccaccggat tggcgtgact
     3841 gtcatattca cagctctgtt ttacattacc aaccctttga ttattcagta aagtaaccgc
     3901 tgtgtaatct aattttttga tcggatccgt tgatggcgta gcgctgacag ataatgtttt
     3961 gtcgttactt ttcgtggttt tacccagcgg atacccctga tcatcatagc gatattccat
     4021 ctgcatctgt ttgctgctgg ctttaatcac aaagccatta tcatcggttt cccagctcac
     4081 cccggcagaa ggtaattctg ctagctggca ttttccctgc aaacgaactc tcttctccag
     4141 tgtctgggca tcacggtaat aattggcgtc cagtaccagg gcaaccacgg tattattttc
     4201 cagatccagt aattcgagtg aatcaaaaca gccttcttcc gacaaagtcc ccgaaacacg
     4261 tttcgtcact tcaccttgct catccattaa tgtctgagtg aaatctttta ccggaccacg
     4321 cagcggatca aaatcgaatt cattagagaa actggccatc tcaggggtaa atgaaagtgt
     4381 tacctctgtg cggtcacatc ctgtgaggaa tatcgcgagt aagcatggca gtaatttgta
     4441 tttcacaaca gtcaccagag agtagagatg attctcaatc atagtagcaa atacagtact
     4501 ttacacgtta aatgctatgc ttaaagaagt tatcttcgcg taaggagctt atgatgaaac
     4561 tctcaacctg ctgtgccgcg cttctgctcg ccctcgcctc acccgcggta ctcgccgcgc
     4621 cgggatcctg tgagcgcata cagagcgata tatcacagcg cattatcaat aatggcgtac
     4681 ccgaaagcag cttcacgtta agtattgtac ccaatgacca ggttgatcag cctgattccc
     4741 aggtcgtcgg gcattgcgct aatgatacgc ataaaattct ctacacccgc accaccagcg
     4801 gtaacgtctc tgctcccgcg cagtctaccc aggatggcgc gcctgccgaa ccgcagtaat
     4861 acattatcga ctgcacgccg gatacgacac atccggcgat ttgaacatac aacataatcc
     4921 caccttatta ctcataccct tctattgata tggattaata attcttaacc caaaataggt
     4981 agactccctc tattgttagc gcgctaaata ttcaatatat aaacttttat ataacgataa
     5041 agaacaggga gtgagttatg tccaaaaatg aacaaatggt gggcatcagc cgcagaacac
     5101 tcgttaaatc taccgcgata ggttctctgg cgctggctgc aggcggtttt tctttaccgt
     5161 tcaccctgcg cagtgcagcg gcagcggtac agcaggccag cgagaaagtg gtctggggtg
     5221 cctgttccgt caactgtggt agccgctgtg cacttcgtct gcatgttaaa gataatgaag
     5281 tgacctgggt ggaaactgac aacaccggca gcgatgagta tggcaatcat caagtacgcg
     5341 cctgtttgcg cggtcgctcc atccgtcggc gtattaatca tcccgaccgc ttgaattacc
     5401 caatgaaacg cgtgggcaaa cgcggcgaag gcaaattcga acggattagc tgggatgaag
     5461 ccctggatac tatcgccagt agcctgaaaa aaaccgtcga acaatatggc aatgaggctg
     5521 tatatattca gtactcttca gggatcgttg gcggcaatat gacccgctct tcgccatccg
     5581 cctcggcggt caaacgcctg atgaactgct acggcggttc actcaaccag tatggctcct
     5641 acagcaccgc gcaaatctcc tgtgccatgc cctacaccta cggcagtaat gatggcaaca
     5701 gcaccacgga tattgaaaac agcaagctgg tcgtgatgtt tggtaacaac ccggctgaaa
     5761 cccgcatgag cggcggtggc atcacttatc ttcttgaaaa agcgcgcgag aaatcgaacg
     5821 caaaaatgat tgttatcgat ccgcgatata ccgatacggc tgctggtcgt gaagatgaat
     5881 ggcttcctat tcgcccgggc accgatgccg cgctggttgc gggtattgcc tgggtattgg
     5941 ttaacgaaaa tctcgttgat caaccttttc tcgataaata ctgcgtcggc tatgacgaaa
     6001 aaaccttacc cgcagatgca cccaaaaatg gtcactataa agcctatatt cttggtgaag
     6061 gtgatgataa aacagcgaaa acgccgcagt gggcttcgca aattacaggt atcccggtag
     6121 accgtatcat caaactggcg cgtgaaattg gcacagcaaa acccgcttat atctgccagg
     6181 gctgggggcc gcaacgccag gcaaacggcg agctgacggc acgcgctatt gctatgttgc
     6241 ctattttgac gggcaatgtc ggcatcagcg gcggaaatag tggtgcgcgt gaatcgacct
     6301 acaccattac catcgaacgc ctgccggtgc tggataatcc ggtcaaaacg tcaatctcct
     6361 gcttcagttg gacagatgct atcgatcatg gcccgcaaat gacggcaatc cgcgacggcg
     6421 tccggggcaa agataaactg gatgtgccca ttaagttcat ctggaactac gcgggaaata
     6481 ccctggttaa tcagcattct gacatcaaca aaacgcatga aattctgcag gacgaatcga
     6541 aatgcgaaat gattgtggtc atcgaaaact ttatgacctc atcggcaaaa tatgccgaca
     6601 ttctgctgcc agacctgatg accgttgagc aggaagatat tattcctaac gactacgccg
     6661 gtaacatggg atatctcatt ttcctccagc ctgtcaccag cgaaaaattc gaacgcaaac
     6721 caatttactg gatcctgagt gaagtcgcga aacgtcttgg acctgacgtc tatcaaaagt
     6781 tcactgaagg tcgcacgcag gaacaatggt tacaacatct gtacgccaaa atgcttgcca
     6841 aagatccggc gttaccgtct tacgacgaat taaaaaaaat gggtatctat aagcgtaaag
     6901 atcccaatgg tcattttgtc gcctacaaag catttcgtga cgaccccgag gcaaatccac
     6961 ttaaaacgcc ttccggtaag attgaaattt attccagcaa gctggcggaa attgcccgta
     7021 cctgggaact ggaaaaagat gaagtgataa gcccactgcc ggtttatgcc tcaacctttg
     7081 aaggctggga ctcccctgaa cgtagtacct ttccactgca actgtttggt ttccattaca
     7141 aatcccgtac tcactcgacc tacggcaaca ttgatgtcct gaaggctgcc tgccgtcagg
     7201 aggtgtggat caaccctata gacgcacaga aacgtgggat tgccaacggc gatatggtgc
     7261 gggtgtataa ccatcgtggc gaagttcggc taccagccaa agtgacacca cgcattctcc
     7321 ctggggttag cgctatgggc cagggagcct ggcacgaggc caatatgtct ggcgataaaa
     7381 tcgaccatgg cggctgtgtg aatacgctaa ccactctgcg cccttcacca ctggcgaagg
     7441 gaaacccgca gcacactaat ctggtcgaga tcgaaaaaat ataacccact acaaccataa
     7501 attctggcat gacattttgt ttgaaaagca ataagtgagt aatgatgaaa atccatacca
     7561 cagaggcgct catgaaggct gaaatcagcc gtagaagtct gatgaaaacc tccgcacttg
     7621 gcagtctcgc gctggcaagc agtgctttca ctctgccatt ttcccaaatg gtccgagcgg
     7681 cagagacacc ggtagaagag aaagcggtct ggagttcctg caccgttaac tgcgggagcc
     7741 gctgtctgtt acgtttgcat gtgaaagatg acaccgtgta ctgggtggag tctgatacga
     7801 caggagacga cgtttacggt aatcatcagg ttcgggcatg tttacgcggg cgctctatcc
     7861 gccgacggat gaatcatcct gataggttga aatatcccat gaagcgcgtc ggcaagcgcg
     7921 gtgaaggtaa atttgaacgg ataagttggg atgaagccct ggataccatc agtgataatc
     7981 ttcggcggat ccttaaagat tacggcaatg aggctgtaca tgtcctgtac ggaacaggcg
     8041 tagatggcgg aaacatcacc aactcgaacg tcccgtaccg tctgatgaac tcttgcggtg
     8101 gttttctcag tcgctatggc agctacagta ccgctcaaat cagtgccgca atgagttata
     8161 tgttcggtgc caatgatggc aacagcccgg atgatatcgc caatacgaaa ctggtcgtta
     8221 tgttcggaaa taacccggca gaaacccgga tgagcggcgg tggtgtcact tactacgtcg
     8281 agcaagcccg agaacgttca aacgcacgca tgatcgtcat cgatccacgt tataacgaca
     8341 cagctgccgg gcgtgaagat gaatggctgc caattcgccc aggcaccgat ggcgcactgg
     8401 cctgtgcgat tgcctgggta ctgattactg aaaacatggt cgatcagcca tttctcgaca
     8461 aatattgtgt tggttacgat gaaaaaacgc tgcccgccaa cgcaccacgt aacgcgcatt
     8521 ataaagccta tattctgggc gaagggcctg acggcatagc taaaacgccg gaatgggcag
     8581 caaaaatcac cagcatcccg gcagaaaaaa ttatccagtt ggcacgagag atcggttcag
     8641 caaaacctgc ttatatttgt cagggttggg ggccacaacg acactccaac ggcgaacaaa
     8701 cgtcccgcgc tattgccatg ctttccgttc tcaccggtaa cgtcggcata aacggcggca
     8761 actcaggtgt acgcgaaggt agctgggatc tgggggtaga atggttcccg atgcttgaga
     8821 atcctgttaa aacgcagatt tccgtcttta catggacaga tgccatcgac cacggtacgg
     8881 aaatgaccgc gacccgtgat ggtgttcgtg gaaaagaaaa actggatgtc cccatcaagt
     8941 ttttatggtg ctacgccagt aacacattga tcaatcaaca tggcgacatc aatcacaccc
     9001 atgaggtgct tcaggatgac agcaagtgcg agatgattgt tggcattgac cacttcatga
     9061 ctgcctcggc taagtattgc gatatcctgt tgcccgacct gatgccaaca gagcaagaag
     9121 accttatctc tcacgaatct gccgggaata tgggctatgt gatcctcgcc caacccgcaa
     9181 cctcagcaaa atttgaacgc aaacccatct actggatgct gagcgaagtt gccaaacgct
     9241 taggaccaga cgtttatcaa acctttactg aaggccgcag tcagcacgaa tggatcaaat
     9301 atctccatgc gaaaacgaag gaacgtaacc ctgagatgcc cgactacgag gagatgaaaa
     9361 cgaccgggat ctttaagaaa aaatgcccgg aagaacacta cgtcgctttc cgcgcattcc
     9421 gtgaagatcc acaggcaaac ccgttgaaaa caccttcggg gaaaatcgaa atttattctg
     9481 aacgactggc gacgattgcg gatacctggg aattgaaaaa agatgaaatt attcatcccc
     9541 ttcctgcgta taccccaggt tttgatggct gggacgatcc cctgcggaaa acctatccac
     9601 tgcagctaac gggcttccac tacaaagcgc gtacccactc cagctacggc aatattgatg
     9661 tgttacagca ggcctgccca caagaggtgt ggatcaaccc tattgatgct caggcacgcg
     9721 gtatccgtca tggcgatacc gtgcgggtat ttaacaataa tggagaaatg ctgattgccg
     9781 caaaagtgac tccgcgtatt ctgcctggcg ttaccgccat cgggcaaggt gcgtggctta
     9841 aagcggatat gtttggtgac cgggtcgatc acggcggcag tatcaatatt ctgacctctc
     9901 accgcccttc accgctggca aagggaaacc cgtcgcacag caatcttgtc cagatcgaaa
     9961 aggtttaagg agtagcccat gaccacacaa tatggatttt ttatcgattc cagccgctgt
    10021 actggctgta aaacctgcga actggcgtgc aaagatttta aagatcttgg cccggaagtc
    10081 agtttccgcc gtatttacga atatgctggc ggcgactggc aggaagataa tggcgtctgg
    10141 caccaaaacg tgttcgctta ttatctctcc atctcctgta accattgcga tgaccccgca
    10201 tgtacaaaag tctgccccag cggcgcaatg cataagcgtg aagatggctt tgtggtggtt
    10261 gatgaggatg tttgtatcgg ctgccgctac tgccacatgg cttgtccgta cggcgcgcca
    10321 cagtacaatg ctgaaaaagg gcacatgacg aagtgcgatg gttgttattc gcgcgtcgcc
    10381 gaggggaaac aacccatatg tgtcgaatcc tgcccgctgc gtgcacttga gttcggtcca
    10441 attgaagagc tgcgccagaa gcacggcact ctggctgccg tcgcgccact accgcgcgcg
    10501 catttcacaa aacccaatat cgttatcaaa cctaacgcca acagccgccc gaccggcgat
    10561 accactggtt atctggctaa tccggaggaa gtgtaaaatg ggaaatggat ggcatgaatg
    10621 gccactggtt atctttaccg tattgggtca atgcgtagta ggcgcgctga ttgttagcgg
    10681 aattggctgg ttcgccgcca ataatgacgc agaccgtcag cgtattgtcc gtggcatgtt
    10741 tttcctctgg ctgttgatgg gcatcgggtt tattgcctct gtcatgcacc tgggatcgcc
    10801 actgcgagcg tttaactcgc tgaatcgaat cggagcttct ggtctgagta acgagattgc
    10861 tgccggttcc atattttttg ccgttggcgg attatggtgg ctggtggcag tcatcggtaa
    10921 aatgccgcaa gcgttgggga aactctggtt acttgtcagc atggcgctcg gcgtcatttt
    10981 cgtctggatg atgacctgcg tgtatcaaat cgacacagtg ccaacctggt ataacggtta
    11041 cactacgctg gctttcttcc tgacagtatt gttgagcggt ccaatacttg ctgccacaat
    11101 tctgcgggca gcccgcgtta catttaatac cacaccattt gccatcatta gcgttctggc
    11161 gctgatcgct tgtgccgggg tgattgtgct gcaaggtttg tctctggcgt ctattcactc
    11221 atccgtacaa caagccagcg cactggtacc agattacgcc tccttacagg tctggcgtgt
    11281 ggtattgtta tgcacaggtc taggatgctg gctttgtccg ctcattcgtc gccgtgaacc
    11341 tcacgttgcc ggacttattc tggggctaat cctcatactt ggcggtgaga tgatcgggcg
    11401 tgtgctcttt tatggcctgc acatgacggt aggaatggct attgcaggtt aacaaattgg
    11461 tgcggggcaa cccgcacatt tcaggatgtt aggaatgacc catttttcac agcaagataa
    11521 tttttctgtc gcggcgcgcg tgttgggcgc gctgttttat tacgctccag agagtgcgga
    11581 agccgcacct ctggttgcgg tactcacccg tgacggctgg gaaactcagt ggcctctacc
    11641 agaagcgtca ttagcgccgc tggtgacggc ttttcagaca cagtgtgaag agactcacgc
    11701 ccaggcctgg cagcgtttgt ttgtcggccc gtgggcactg ccgtctccgc cgtggggatc
    11761 ggtctggctg gatcgcgaat ctgtgctgtt tggcgattca acattggcac ttcgtcagtg
    11821 gatgcgcgag aaaggcattc agtttgaaat gaagcaaaac gaaccggaag atcattttgg
    11881 ttcactgctg ttgatggctg cgtggttggc ggagaatggt cgccagacag aatgcgaaga
    11941 attgctggca tggcaccttt ttccgtggtc aacacgattt cttgacgttt ttatcgaaaa
    12001 agcagaacac cctttctacc gtgcactggg ggaactggct cgcttaacgc tggcgcaatg
    12061 gcaatcacaa ctgttgattc ctgtcgcggt taaaccactc tttcgatagg atcaccgtaa
    12121 tattgccgga tggtggcgat tacgcgcaat tcggcaataa taggttacag tgtcacgttt
    12181 ttttatctct taaagcacgc actgcttttg cggctggcct cttttgccgc aaaatagtcg
    12241 cccgtgtttc attgcccatt tctgctcatg catcatctac acatctatcc ggatctgcgc
    12301 actatgttcc gccgtctgct tatcgcaaca gtcgtcggta ttcttgcggc ctttgccgtt
    12361 gccgggtttc gtcatgcaat gctgctactg gagtggttgt tcctcaataa tgactcgggc
    12421 agtctggtca atgcagcgac aaatctttcc ccatggcgac ggctgctaac tccggcgctc
    12481 ggcggactgg cggcgggttt gttgctgatg ggctggcaga aatttaccca gcaacgccct
    12541 catgcgccga ccgattacat ggaagcgttg caaaccgatg ggcagtttga ttacgcagca
    12601 agcctggtta aatcgcttgc ctctctgctg gtagtaacca gcggcagtgc aattggtcgc
    12661 gaaggtgcga tgattctttt agctgccctt gccgcctcct gttttgccca acgttttacg
    12721 ccacgccagg agtggaaatt atggatcgcc tgtggagctg cggcgggaat ggctgcggcc
    12781 tatcgcgccc cgcttgctgg cagtttattt atagccgaag tgctgtttgg cactatgatg
    12841 ttggcctctc tcggcccggt gattatttcc gccgtcgtgg cgttgctggt tagcaatctg
    12901 attaatcata gcgacgcgtt actctacagc gtacaactct cagtgacggt tcaggctcgt
    12961 gactatgcgc tgattatcag tacaggtgtg ctggcaggtc tgtgcggacc actgttgtta
    13021 acgttaatga acgcctgtca tcgtggattt gtaagtctca aacttgcgcc gccctggcaa
    13081 ctggcactgg gcgggttgat cgtgggtctg ctttccctgt tcacacctgc agtgtggggc
    13141 aacggctata gcaccgtaca atccttttta accgccccgc cactgttaat gatcattgcc
    13201 gggatcttcc tttgtaaact gtgtgccgtg ctggcgagta gcggttccgg cgcacccggt
    13261 ggtgtcttta caccgacgct atttatcggt cttgccattg gcatgttgta tggtcgtagc
    13321 ctgggattat ggttccctga tggcgaagaa attacgcttt tactcggatt gaccgggatg
    13381 gcgacactgt tggcggcaac cacgcacgcg ccgattatgt cgacgttgat gatatgtgaa
    13441 atgaccgggg agtatcagct actccccggt ttattgattg cctgcgtaat tgcgtcggta
    13501 atttcgcgga cgttacaccg tgactctatc taccgccagc acactgcgaa gcatagctaa
    13561 gcggatgtat tgccccagtt cacgctgttc ggcgcgcggc agataaggca gttcaccaat
    13621 gagcggtgcc ggaagttttt taccgagcac atcaatgatt tccgcataat gcgccagtcc
    13681 tggattgatt cggttagcca cccagccaat gagcggcagc ccgtcgttgg caatcgcctg
    13741 agctgttagt agtgcatggt taatgcaacc ttcctgaata ccgacaacca tcaataccgg
    13801 cagttgttcc tgcaccaccc attcagagag cggacgcaaa tcattcatca gactgcgcca
    13861 gccgccagtc ccttctacca cgacatgatc gactttatcg gtcaggtttg ccaggccgtt
    13921 tgaaatgagg gtgtaattga ttgggcaact gtgcgccacg ctactttctt cttcgcttaa
    13981 cgcgatagga ttaactgctt cataaggcag ttcgatggtt gaaacactct gcaacaccag
    14041 ggcatcttta ttacgcagcc cttcgggtgt ctctttgctc cccttcgcta cgggtttata
    14101 tcccgcaacc gtttttccct gggaggctaa cgcttgtagc aatgcgcggg aaaccaccgt
    14161 tttccctaca gaagtgtctg taccggtaat aaagaaacgc ttcagcatca ctaactccac
    14221 cgttatgctt cacaaatata aaccaggaaa ataattaacc ttgaaagtct aagttatgct
    14281 ttcctggccc aaattgagat agcgcaaatt ttggtagaac agttaaaaaa tgttaaccct
    14341 gcaacagacg aatcaacaaa gaaccgttat acatcgcgtc ttttaccagc gcagcccctg
    14401 ccatcgtacc ctggttagaa aactgagtac tctcaacgct aatgtgctga ctatacgcag
    14461 gaagggcctg ctgacggatg ctgtccgaga tgaccgggaa gaggatatct gccgctttac
    14521 ttaacggtga gccaatcagt attttttgtg ggttaaataa attcaccatg atggcaagaa
    14581 tgcgccctac atgcgcgccc accccggtaa tgatgtcttt tgccagtaga tcgccgcgca
    14641 atgccgcctg acacaatgaa tccacggtta acggctgtcc atgtaacatc gagctcatgg
    14701 attgattgag acgcagctgt gccagctcaa gaatactgtc cacactggcg atggtttcga
    14761 ggcagccgtg attcccgcaa taacagcgtt tcccatacgg gtcgacctgc gtgtgaccta
    14821 tttccacgag gctactgctg cctgcgtgta gcagatgacc atcggtaatg acgcccgccc
    14881 ccacgttgtg atcgataacc acctgaatca catcgcgcgc cccgcgtgag gcaccaaaca
    14941 aggcctcagc catcgtccat gcgctgatat catgctgaat ataaaccggt acgccggtat
    15001 gctgctccag cgcctcgccg agcggcatct cttttacatc ctcgtagaac ggcatgcgat
    15061 gtacaatacc attttccgta tcaataattc ccggcaaggt tatggcaatc gaagtcagac
    15121 gctcaagttt tttctggtgg cggataaaaa actgatcgat atgggaaatg atacgatcca
    15181 gcaatggcga gtcatctttt aacgccagtt cctgcgactc ctccactacc agtttgctgc
    15241 tcagatcgcg caaagcaagg aaaatctccc cgcgactaat gcgcagagaa agatagtgcc
    15301 aggcttcagt ttcaaccacc agccccactg ccggacggcc acggttcccc gcttctttga
    15361 tttccagctc ttgcaccagg tgtgcttcga gcatctcacg gacaatttta gtgatactgg
    15421 caggagccag ctgcgccaga cgggaaagat cgatacgcga gactggacca agctgatcaa
    15481 tcaggcgata aaccgcgccc gcgttggtct gctttatttg atcaatgtgc ccaggctgat
    15541 tttcagcaac caccgcatac tccctatatt ttcgcgctcc gaaataatct gtaggctatg
    15601 gtgaagcact tcaatacgtg tcgtcaaatt tttacttagg catgtgatta acagcacatt
    15661 tttcgggctt tttcgctgaa atttccctca cctgagagca ttcagcagat gaatacgaaa
    15721 gttacgcgca gccggacttt gttcatggtg tttcggccag accaaccaca tttcagaaac
    15781 cgcatcctct tcagcaatcg gcacccagcg catttcgttg agctgaacac gtttaaatga
    15841 cgcaggcaaa attgaaacac ccagaccagc ggaaaccaga ccgatgatgg tcattgcctc
    15901 acccacctcc tgagtgatga cgggcgtcaa attgtaacgt cgcatcagcc cgagaatatc
    15961 gtcatacagc cctgtcccta cgtgcggatc aaaaaagaca aagggttctt tcgccagttc
    16021 agccagcgtt acactcgggt tatttgccaa gggatgatcg tgcgggatca tcgccataag
    16081 cggttcatgg acgatgactg cgtgttcaag cgtctccggt aacgctgtgt tacgcagcaa
    16141 tcccatatcc agcgttcctt caatgagcgg agcgatttgc tcgcgggtgt tcatttcgcg
    16201 ggtttgtaaa tggacatcgg gataatcacg gcgaaacagc gataacgtat cggacaccgc
    16261 ccgaataaaa ggagcggacg aagtaaaacc aatgcgcaac tcccccgctt caccctgatg
    16321 cagtctttca gcgcgagcgg cagcgtcatc caccatagac aggatttgcc gactatctgc
    16381 aagaaactgt tttcctgctg ccgtcagcaa tacactgcga ttagttcgtg ccagcagtcg
    16441 ggcaccaatt tgttgctcca gcgcctgaat ctgctgactt agcggcggtt gcgaaatatt
    16501 caggcgggca gcggcgcgcc cgaaatgcag ctcttctgca acagcaacaa agtaacgcag
    16561 atgacgaagt tcaatattca tatttaaaac atcttatttg agattattaa tatattagac
    16621 agaacaattc gattttccta atctattcat aagcctgaac cacaggcata tttagcaagg
    16681 atttcaagtg agccgtacta caactgttga tggcgctccg gcaagcgaca ctgacaagca
    16741 aagcatttct cagccaaatc aatttattaa acgcggtacg ccgcaattta tgcgcgtcac
    16801 cctggcgctg ttctctgccg gactggcaac cttcgcactt ctctattgtg tgcaacctat
    16861 ccttccggtg ctttcgcagg agtttggctt aacgcccgcg aacagtagta tttcactgtc
    16921 catttctacg gcgatgttgg ctatcggttt gctgtttact ggcccgttat ccgatgccat
    16981 tggtcgcaaa ccagtgatgg tcacggcgct actgttggcc tccatttgta cgttactttc
    17041 gacaatgatg accagctggc acggcatttt gattatgcgc gccttgattg ggctttcgtt
    17101 aagtggcgta gcagctgttg gcatgactta tcttagcgag gaaatccacc ccagtttcgt
    17161 ggccttttcg atggggttgt atatcagcgg caactcaatt ggcggcatga gcggacgctt
    17221 aattagcggt gtcttcacgg actttttcaa ctggcgaatt gcgctggcgg caatcggttg
    17281 tttcgcgctg gcctcggcgt taatgttctg gaaaatcctc cctgaatcac gccattttcg
    17341 cccgacttca ctgcgcccta agacgttatt catcaacttt cgtctgcact ggcgtgaccg
    17401 gggattaccg ttattgttcg cagaaggctt tttgctgatg gggtcgttcg tcacgctgtt
    17461 taattacgtc ggctatcggt tgatgctctc cccctggcat gtcagccagg ccgtggttgg
    17521 cttattatcg ctggcttatt tgaccggtac atggagctca cccaaagccg gaaccatgac
    17581 cacccgctat gggcgcggtc cggtgatgtt gttttcgacg ggggttatgc tgtttggttt
    17641 attgatgacc ttattcagct cgctgtggct gatctttgcc ggaatgttac tcttctcagc
    17701 cggattcttc gcagcccact cagtagccag cagctggatc ggcccccgcg caaaacgcgc
    17761 taaaggccag gcctcctcgc tgtatctgtt cagttactat ctggggtcga gtattgccgg
    17821 gacgctgggt ggtgttttct ggcataacta tggctggaac ggcgtcggcg catttattgc
    17881 tctgatgctg gtcattgctc tgctggtcgg gacgcgtttg catcatcgtc tgcacgcctg
    17941 a
//
LOCUS       DAZUCC010000095        17660 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZUCC010000095 DAZUCC010000000
VERSION     DAZUCC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.095"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            27..752
                     /gene="yjjQ"
                     /locus_tag="ACHY09_002733"
     CDS             27..752
                     /gene="yjjQ"
                     /locus_tag="ACHY09_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3885688.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            710..1387
                     /gene="bglJ"
                     /locus_tag="ACHY09_002734"
     CDS             710..1387
                     /gene="bglJ"
                     /locus_tag="ACHY09_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFS3885689.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1424..2212)
                     /gene="fhuF"
                     /locus_tag="ACHY09_002735"
     CDS             complement(1424..2212)
                     /gene="fhuF"
                     /locus_tag="ACHY09_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFS3885690.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNTLSSQLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2353..2589
                     /gene="yjjZ"
                     /locus_tag="ACHY09_002736"
     CDS             2353..2589
                     /gene="yjjZ"
                     /locus_tag="ACHY09_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFS3885691.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     AMLYHKQLRHYVLLPSCLALIAGIMLMIMNLKQG"
     gene            complement(2628..2714)
                     /locus_tag="ACHY09_002737"
     tRNA            complement(2628..2714)
                     /locus_tag="ACHY09_002737"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2678..2680),aa:Leu,seq:cag)
     gene            complement(2914..3945)
                     /gene="rsmC"
                     /locus_tag="ACHY09_002738"
     CDS             complement(2914..3945)
                     /gene="rsmC"
                     /locus_tag="ACHY09_002738"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFS3885692.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLSDYAPLNKMDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4048..4461
                     /gene="holD"
                     /locus_tag="ACHY09_002739"
     CDS             4048..4461
                     /gene="holD"
                     /locus_tag="ACHY09_002739"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFS3885693.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPIVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4430..4876
                     /gene="rimI"
                     /locus_tag="ACHY09_002740"
     CDS             4430..4876
                     /gene="rimI"
                     /locus_tag="ACHY09_002740"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS3885694.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4891..5568
                     /gene="yjjG"
                     /locus_tag="ACHY09_002741"
     CDS             4891..5568
                     /gene="yjjG"
                     /locus_tag="ACHY09_002741"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFS3885695.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5659..7248
                     /gene="prfC"
                     /locus_tag="ACHY09_002742"
     CDS             5659..7248
                     /gene="prfC"
                     /locus_tag="ACHY09_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS3885696.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQSAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7641..8246
                     /gene="osmY"
                     /locus_tag="ACHY09_002743"
     CDS             7641..8246
                     /gene="osmY"
                     /locus_tag="ACHY09_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFS3885697.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8373..8534
                     /gene="ytjA"
                     /locus_tag="ACHY09_002744"
     CDS             8373..8534
                     /gene="ytjA"
                     /locus_tag="ACHY09_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFS3885698.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8656..9729
                     /gene="yjjU"
                     /locus_tag="ACHY09_002745"
     CDS             8656..9729
                     /gene="yjjU"
                     /locus_tag="ACHY09_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFS3885699.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            9726..10505
                     /gene="yjjV"
                     /locus_tag="ACHY09_002746"
     CDS             9726..10505
                     /gene="yjjV"
                     /locus_tag="ACHY09_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS3885700.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLKKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYTLFNVP"
     gene            complement(10723..11586)
                     /gene="yjjW"
                     /locus_tag="ACHY09_002747"
     CDS             complement(10723..11586)
                     /gene="yjjW"
                     /locus_tag="ACHY09_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFS3885701.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALWERG
                     VSRLIFPALYL"
     gene            complement(11558..13108)
                     /gene="yjjI"
                     /locus_tag="ACHY09_002748"
     CDS             complement(11558..13108)
                     /gene="yjjI"
                     /locus_tag="ACHY09_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFS3885702.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13366..14145
                     /gene="deoC"
                     /locus_tag="ACHY09_002749"
     CDS             13366..14145
                     /gene="deoC"
                     /locus_tag="ACHY09_002749"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS3885703.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14223..15545
                     /gene="deoA"
                     /locus_tag="ACHY09_002750"
     CDS             14223..15545
                     /gene="deoA"
                     /locus_tag="ACHY09_002750"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFS3885704.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15597..16820
                     /gene="deoB"
                     /locus_tag="ACHY09_002751"
     CDS             15597..16820
                     /gene="deoB"
                     /locus_tag="ACHY09_002751"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS3885705.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            16877..17596
                     /gene="deoD"
                     /locus_tag="ACHY09_002752"
     CDS             16877..17596
                     /gene="deoD"
                     /locus_tag="ACHY09_002752"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS3885706.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4246 a         4492 c         4749 g         4173 t
ORIGIN      
        1 ttttagacag tggatgtgga ggaaatatgt tgccaggatg ctgcaaaaat ggaattgtta
       61 tcagtaaaat acctgttatg caagcagggt taaaagaggt catgaggact cacttccctg
      121 aatatgaaat aatatccagc gcctctgcgg aggaccttac cttattacaa ttacgtcgtt
      181 ccggattagt cattgctgat ttagccggtg aaagtgaaga tccacgttct gtttgtgaac
      241 attattattc tttaatctca caatatcggg aaattcactg ggttttcatg gtgtcacgct
      301 cctggtattc ccaggcagta gaactgctta tgtgccctac ggcgacgtta ttgtctgatg
      361 ttgaacccat tgagaatctg gtcaagaccg tacgttccgg caatacgcac gcagagcgta
      421 tcagcgccat gctgacctct ccggcaatga ctgaaactca tgattttagc tatcgctccg
      481 tcattctcac tctttcagag cgcaaggtac tgcggctatt aggtaaagga tggggcatca
      541 accagatagc ttcattgctt aagaaaagta ataaaactat cagcgcccaa aaaaacagtg
      601 cgatgcgtcg actggcaatt cacagcaacg ctgaaatgta tgcatggata aatagcgcgc
      661 agggtgcaag agaacttaac ttgccttctg tttatggaga tgccgcagaa tggaacacag
      721 ccgaattaag aagagaaatg tcgcactcat agaaaaatgc gtcatgagca gtatcggtat
      781 tgagagttta ttcagaaagt ttgcgggtaa cccttataag ctccatacct ataccagtca
      841 ggagtcattt caggatgcca tgtcgcggat ctcgtttgcg gcggtcattt tttctttttc
      901 tgccatgaga agtgagcgca gagagggatt atcttgcctg actgaactgg cgattaagtt
      961 tccgcgtacc cggcgtttag ttattgcgga tgatgatatt gaagctcggc tgattggttc
     1021 attgtcgcca tcaccgttgg acggtgtatt aagtaaagcg tcaacgctgg agatttttca
     1081 tcaggaactc tttttgtcat taaatggtgt acgtcaggcg accgaccgac tgaacaatca
     1141 gtggtacatt aatcaaagcc gaacgctaag cccgacggag agagaaatat tgcgctttat
     1201 gtcgcgtggc tactcaatga cacaaattgc cgagcagctt aaacgcaata tcaaaacgat
     1261 ccgtgcacat aaatttaatg tgatgtcgaa actgggcgtc agttctgatg cagggttgtt
     1321 ggaggccgca gatattctgt tatgtatgcg gcattgcgaa gcaagtaatg tgttgcatcc
     1381 ctattaatcc acatgatgcc gggtttactt ccccggcagt gcttcatttc agcgtacaat
     1441 cgccacattg ctgcacatcc ggtaagcgat aacgctggca gcaagtgcgg cgcaccagca
     1501 ggccgtcgcg cagtaccacg gtacgccaga gtggattatc ttcaccgttc gtgagcattt
     1561 tctcaaaaaa gagggcatgg cgcagcgatt caaccgtcgc ctcgccaagc agctgtttca
     1621 tctcagtcag ataccagttg atcaaataac cggtattgct ccagataagt ttgccgttga
     1681 tctctcccgt cgcttctaat gcctgcacta ccggaacaag cgcctggctg attaacgttt
     1741 ccattcgctg ctgcggcgaa tgtagtgttg cgtttttatc ttcacacaca tcgacccaga
     1801 aacaggcggc gcgtccggtt tcgtgaaact caacgtggaa atgttccggc gacacatcta
     1861 atgctttttc ctgcgtcagc agcgccaaca ttaatggtgg caccatcagg ccgatatacc
     1921 attgtgccca tagtgagatc agcggtttgt tctcgcggat catcaagggt tggttgcggt
     1981 agatatgatc ggaatagacc gccagttgag aacttagcgt attcggtgat gaccattgcg
     2041 ccagcgtcat ggcacggagc ggtgcgggtt catccagacg gataaactcc agcaaatgtt
     2101 cacgatgttc ggcgatcgtc gcccgtacgg cttgtgcaag cccagcatcc tgcggctgga
     2161 gatgcgttcg ccagataaca tcttcataga gcggtgcgga acgataggcc ataatcgtga
     2221 tagtaatcta aatgataatg attgctaatc ctagcgatag gtttacccga tagcaaggga
     2281 tttctctggc ttgcaaatga taaaaattat catatgatat tggttatcat tatcaatgaa
     2341 agagatgaaa ccatgttgca acgtacgctg ggcagtggct ggggagtgtt gctgccggga
     2401 ttgctgattg cagggctgat gtatgcggat ttatcgccgg atcaatggcg gattatcatt
     2461 ctgatgggat tagtattgac gccggcgatg ctgtatcaca aacagttgcg gcattacgtt
     2521 ttgctgccat cgtgcctggc gcttattgct ggcatcatgc tgatgataat gaatttgaag
     2581 cagggatgat aaaacaggga agaaacaaga aaggaagtaa agataattgg tgcgaggggg
     2641 gggacttgaa cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat
     2701 tccgccacct tcgcataccg tcaattctta aaaagaattg ctaccacgga ggcgcattct
     2761 agtggttttc agcttttcgt caatagttaa ttatcgacag aggtgtaatt gctggaaaaa
     2821 tgtccatcag cggattgtgg ataattcgtt tgtcggtcag ataaggcgtg tacgccgcat
     2881 ccgacacggt atgcggcgac atatatcaga tactcaacct ttcttcgcct ggcgggtcat
     2941 aatggcgcga tacaccttga agcgcccggt ttgcgcgatc acttcgtgga agccaaatgt
     3001 ctcatccagc acgtccggat aaggcaggaa ggcgttcgct acaattcgca gctcgccgcc
     3061 gctattaaga tgacgcaccg cgccgcgaat cagcgtttgc gcggcatcca ggctggtttg
     3121 catcccatcg tggaacggcg ggttggagat gatcatatca aaacgacctt tcacctcgga
     3181 aaagacgtta ctggcaaaga cttcaccttc aacaccgttg gccgcaagtg ttgcgcggct
     3241 ggcttctacc gctggtgcag agacatcgca cagagtgaga cgaattttcg gcgaatggcg
     3301 cgcaaaggca accgaaagta cgcccgcgcc acagccgaca tccagcactt tacctttcgt
     3361 gtgcggggtt aacgtcgaga gcagcaactg gctaccgaca tccagaccgt cgcggctaaa
     3421 cacgccaggc agcgttttga ccgtcaggcc atcgacgctg tattcgcccc agaatttatc
     3481 ggcatcaaat actggctgtt tttccagacg accaaaatag aggccacagc gacgagcgct
     3541 gtccatttta ttcaacggcg cataatctga cagcatctgc tcggcgctgc gcacgccgct
     3601 gcggttctcg ccaacgacaa aaatatccgt gcccactggc agcagagaaa gtaaattcat
     3661 caactggaac tgggcttccg gtttgttctt cggccagtag taaatcagcg tatcgcaatc
     3721 tgcgacatca tccgccgtgg cgaccagact aaaacgggcg ttatccccca tctggcggct
     3781 taacacctgc cagtggtgga attgctgggt atgagcacgg ctggccgcgg tatctaaacg
     3841 cgcgggcagg tcatcctgta agtctccggc aaaaagaata cggctttgtt cgaaatcatc
     3901 actgtgacgc agcaagactt cacttgccgg ggtaaatgca gacatggaat gctcctcaat
     3961 taatactggc ggcgattata gccatatgtt ggcgcggtat cgacgaattt gctatatttg
     4021 cgcccctgac aacaggagcg attcgctatg acatcccgac gagactggca gttacagcaa
     4081 ctgggcatta cccagtggtc gctgcgtcgc cctggcgcgt tgcaggggga gattgccatt
     4141 gcgatcccgg cacacgtccg tctggtgatg gtggcaaacg atcttcccgc cctgaccgat
     4201 cctatagtga gcgatgttct gcgcgcgtta accgtcagcc ccgatcaagt gctgcaactg
     4261 acgccagaaa aaatcgcgat gctgccgcaa ggcagtcgct gcaacagctg gcggttgggt
     4321 actgacgaac cgctatcact ggaaggcgct caggtggcat caccggcgct caccgaatta
     4381 cgggcaaacc caacggcacg cgccgcgtta tggcaacaaa tttgcacata tgaacacgat
     4441 ttcttccctc gaaacgactg atttaccagc ggcttaccac attgaacaac gcgcccacgc
     4501 ctttccgtgg agtgaaaaaa cctttgccag caaccagggc gagcgttatc tcaactttca
     4561 gttaacgcaa aacggcaaaa tggcggcgtt tgcgattacg caagtggtgc tggatgaagc
     4621 tacattgttc aatattgcgg tcgatcctga ctatcagcgt cagggattgg gaagggcgct
     4681 gctggaacat ctgatcgacg aactggaaaa acgcggcgtg gcgacgctat ggctggaagt
     4741 ccgtgcttca aacgctgccg ccattgccct gtacgaaagt ttaggcttta acgaggcgac
     4801 aattcgccgc aattactacc ccaccacgga cggtcgcgaa gacgccatca tcatggcgtt
     4861 gccaatcagt atgtaagaca aggtggaata atgaagtggg actggatttt ctttgatgcc
     4921 gatgaaacgc tgtttacctt tgactcattc accggcttgc agcggatgtt tcttgattac
     4981 agcgtcacct ttaccgctga agattttcag gactatcagg ccgttaacaa gccactgtgg
     5041 gtggattatc aaaacggcgc gatcacttca ttacagcttc agcacgggcg gtttgagagc
     5101 tgggccgaac ggctgaacgt cgagccgggt aaactcaacg aagcctttat taatgcgatg
     5161 gcggaaatct gtacgccact gccgggcgcg gtttctctgc ttaacgccat tcgtggcaac
     5221 gctaaaattg gcatcatcac caacggcttt agcgccttgc agcaagtgcg tctggaacgc
     5281 acgggcctgc gtgattattt cgatttgctg gtgatttccg aagaagttgg cgttgccaaa
     5341 ccgaataaga aaattttcga ttatgcgctg gaacaggcag gcaatcctga ccgttcacgt
     5401 gtgctgatgg ttggcgacac agctgagtcc gatattctcg gtggcatcaa cgccgggctt
     5461 gcgacctgct ggctgaatgc gcacaatcgc gagcaaccag aaggcatcgc gcccacctgg
     5521 accgtttctt cgttgcacga actggagcag ctcctgtgta aacactgatt gcctcccccc
     5581 cgttgatggg taaaatagcc gcaatttttc gttttcaaca agcgcggcgc gatgccgctt
     5641 actcaagaag aaagaattat gacgttgtct ccttatttgc aagaggtggc gaagcgccgc
     5701 acttttgcca ttatttctca cccggacgcc ggtaagacta ccatcaccga gaaggtgctg
     5761 ctgttcggac aggccattca gagcgccggt acagtaaaag gccgtggttc caaccagcac
     5821 gctaagtcgg actggatgga gatggaaaag cagcgtggga tctccattac tacgtctgtg
     5881 atgcagtttc cgtatcacga ttgcctggtt aacctgctcg acaccccggg gcacgaagac
     5941 ttctcggaag atacctatcg taccctgacg gcggtggact gctgcctgat ggttatcgac
     6001 gccgcaaaag gtgttgaaga tcgtacccgt aagctgatgg aagttacccg tctgcgcgac
     6061 acgccgatcc tcacctttat gaacaaactt gaccgtgata tccgcgaccc gatggagctg
     6121 ctcgatgaag ttgagaacga gctgaaaatc ggctgcgcac cgatcacctg gccgattggc
     6181 tgcggcaagc tgtttaaagg cgtttaccac ctttataaag atgaaaccta tctctatcag
     6241 agcggtaaag gccacacgat tcaggaagtc cgcattgtta aagggctgaa taacccggat
     6301 ctcgacgctg cggttggtga agatctggca cagcagctac gtgacgaact ggagctggtg
     6361 aaaggcgcgt ctaacgagtt cgacaaagag ctgttccttg cgggcgaaat cactccggta
     6421 ttcttcggta ctgcgctggg taacttcggc gttgatcata tgctggatgg cctggtggag
     6481 tgggctcctg cgccgatgcc gcgtcagact gatacccgta ccgtagaggc gagcgaagac
     6541 aaatttaccg gcttcgtatt taaaattcag gccaacatgg acccgaaaca ccgcgaccgc
     6601 gtggcgttta tgcgtgtggt gtccggtaaa tatgaaaaag gcatgaagct gcgccaggtg
     6661 cgtactgcga aagatgtggt gatctccgac gcgctgacct ttatggcggg tgaccgttcg
     6721 cacgttgaag aagcgtatcc tggcgatatc cttggtctgc acaaccacgg caccattcag
     6781 atcggcgaca cctttaccca gggtgagatg atgaagttca ccggtattcc gaacttcgcg
     6841 ccagaactgt tccgtcgtat ccgcctgaaa gatccgctga agcaaaaaca gctgcttaaa
     6901 gggctggtac agctttccga agagggcgcg gtgcaggtgt tccgtccgat ctccaacaac
     6961 gatctgatcg ttggtgcggt tggtgtgctg cagtttgatg tggtggtagc gcgtctgaag
     7021 agcgaataca acgttgaagc agtgtatgag tcagtcaacg ttgccactgc ccgctgggta
     7081 gaatgtgcgg acgcgaagaa attcgaagag ttcaagcgta agaacgaaag ccaactggcg
     7141 cttgatggcg gcgataacct cgcttacatc gctaccagca tggtcaacct gcgcctggcg
     7201 caggaacgtt atccagacgt tcagttccac cagacccgcg agcattaatt cttgcctcca
     7261 gggcgcggta gccgctgcgc cctgtcaatt tcccttcctt attagccgct tacggaatgt
     7321 tcttaaaaca ttcacttttg cttatgtttt cgctgatatc ccgagcggtt tcaaaattgt
     7381 gatctatatt taacaaagta atgacatttc tgacggcgtt aaataccgtt caatgcgtag
     7441 atatcagtat ctaaagccgt cgattgtcat tctaccgata ttaataactg attcagaggc
     7501 tgtaatggtc gttattcatc acccatcgct tttgtgatgg cggccattga cttctgtaga
     7561 gggtgaagtc tctccctatt cagcaatgca acctcgtgtt gccaggctca aattacgagc
     7621 aaacatacag gaataaatcg atgactatga caagactgaa gatttcgaaa actctgctgg
     7681 ctgtaatgtt gacctctgcc gtcgcgaccg gctctgccta cgcggaaaac aacgcacaga
     7741 ctaccaatga aagcgcaggg caaaaagtcg atagctctat gaataaagtc ggtaatttca
     7801 tggatgacag cgccatcacc gcgaaagtga aggcggcgct ggtggatcat gacaacatca
     7861 agagcaccga tatctctgtc aaaaccgatc aaaaagtcgt gaccctgagc ggtttcgttg
     7921 aaagccaggc tcaggccgaa gaggcagtga aagtggcgaa aggcgttgaa ggggtgacct
     7981 ctgtcagcga caaactgcac gttcgcgacg ctaaagaagg ctcggtgaag ggctacgcgg
     8041 gtgacaccgc caccaccagt gaaatcaaag ccaaactgct ggcggacgat atcgtccctt
     8101 cccgtcatgt gaaagttgaa accaccgacg gcgtggttca gctctccggt accgtcgatt
     8161 cccaggcaca gagcgaccgt gctgaaagta tcgccaaagc ggtagatggt gtgaaaagcg
     8221 ttaaaaatga tctgaaaact aagtaattcg tcgtaattcg tcctcccgaa ctttgtcggg
     8281 aggcgcgatg tgcaccacac taaaaatatc gcattgagtg attttcacgc tcatatcaag
     8341 cggtgacatt aactatggta aaggagacgc ttatgtttcg ttggggcatc atatttctgg
     8401 ttatcgcgtt aatcgccgcc gcacttgggt ttggtggtct ggccggtacc gctgcaggcg
     8461 cagctaaaat tgtctttgtc gtcgggatta ttctgttcct ggtgagtttg ttcatgggcc
     8521 gaaaacgacc ctagatttca gaccattaag aaaattttat ccaaagccag tccagcggac
     8581 tggcttttgc ggttttagcg aataataaat tgcgttactt tgtcattctt tcacaacaaa
     8641 aacaggaagg cagaggtggg gcagcgaata cctgtaacgc ttggtaatat tgcgccgttg
     8701 tcgctaaggc cgttccagcc tggacgaata gctctggtgt gcgaaggcgg cggacagcgt
     8761 ggaattttca cggctggcgt gctggatgag tttatgcgcg cgcagtttaa tcctttcgat
     8821 ctttatctcg gcacatctgc cggggcgcag aacctctcgg cgtatatctg caatcagccc
     8881 ggttacgcgc gcaaagtcat catgcgctat accacaaaac gcgaattttt cgatccattg
     8941 cgctttgtcc gtggaggaaa tcttatcgat ctcgactggc tggtggaggc cactgcaagc
     9001 cagatgccgt tgcaaatgga taccgccgcg cggttgtttg acagcggcaa atcgttttat
     9061 atgtgcgcct gtcgtcagga tgactatgcg ccgaattact ttttaccaac caaacaaaac
     9121 tggctggatg tgattcgcgc ctccagtgcg atacctggct tttatcgtag cggagtgtcg
     9181 ctggaaggca ttaactacct ggatggcggg atcagtgatg cgattccggt taaagaggcg
     9241 gcaaggcagg gagcaaaaac cttggtcgtc atccgcaccg tgccgtcgca aatgtactac
     9301 acgccgcagt ggttcaaacg catggaacgc tggctgggtg acagtagcct gcagccgctg
     9361 gtcaatctgg tgcagcatca tgaaaccagc tatcgtgaca ttcagcaatt tattgagaaa
     9421 ccaccgggcc agctgcggat attcgaaatt tatccgccga agccgttgca tagtatcgcg
     9481 cttggcagtc ggattccggc gctgcgtgaa gactataaac ttgggcgttt atgcggtcgt
     9541 tattttctgg caacggtcgg taagttattg actgaaaaag cgccgcttac ccgccatctg
     9601 gtgccggtgg tgacgccgga atcgattgtc attccgcctg cgccagtcgc caacgatacg
     9661 ctggttgccg aagtgatcga cgctccgcag gcgaacgacc cgacatttaa caatgaggat
     9721 ctggcttgat ttgccgtttt atcgacaccc actgccattt tgatttcccg ccttttagtg
     9781 gtgatgaaga ggccagcctg caacgcgcgg cacaagcggg cgtaggcaag atcattgttc
     9841 cggcgacaga ggcggaaaat tttgcccgcg tgcaggcgtt agcggaaaaa tatcaaccgc
     9901 tgtatgccgc attgggcttg catcctggta tgttgaaaaa acatagcgat gtgtctcttg
     9961 atcagctaca gcaggcgctg gaaaggcgtc cggcgaaggt ggtggcggtg ggggagatcg
    10021 gtctggatct cttcggcgac gatccgcaat ttgagaggca gcagtggtta ctcgacgaac
    10081 aactgaaact ggcgaaacgc tacgatctgc cggtgatcct gcattcacgg cgcacacacg
    10141 acaaactggc gatgcatctt aaacgccacg atttgccacg cactggcgtg gttcacggtt
    10201 tttccggcag cctgcaacag gccgaaaggt ttgtactgct gggctacaaa atcggcgtag
    10261 gcggtactat cacctatcca cgcgccagta aaacccgcga tgtcatcgca aaattaccgc
    10321 tggcatcgtt gttgctggaa accgacgcgc cggatatgcc gctcaacggc tttcaggggc
    10381 agcctaaccg cccggaacag gctgcgcggg tgtttgacgt actttgcgag ttacgcccgg
    10441 aaccagaaga tgagattgcc gaagtgttgc taaataatac gtatacgttg tttaacgtgc
    10501 cgtaggccgg ataaggcgtt cacgccgcat ccggcagttg gcgcacaatg cctgatgcga
    10561 cgcttgacgc gtcttatcat gcctacaagt ctgtgccgaa ccgtaggccg gataaggcgt
    10621 tcacgccgca tccggcagct ggcgcacaat gcctgatgcg acgcttgacg catcttatca
    10681 tgcctacagg tctgtgccga tccgtaggcc gtatccggca tgtcacaaat atagcgccgg
    10741 aaatattaac cggctcaccc cgcgctccca taacgcatca gccagttgtt ccacatcctc
    10801 cggcgtggcg ctcggccagc tttgcgcctc gccatacacg ccgtgggcat gaaacgcgtt
    10861 caggcgtacc ggaacatcgc caagtccctt gattaacgcc gccagttctt cgatgtgttg
    10921 caaataatcc acctggccag ggattaccag caaacgcagt tccgccagct taccgcgctc
    10981 tgccagcaaa cagatgctgc gcttgatctg ctgattatcg cgtccggtta attgctgatg
    11041 acattcgctc ccccacgctt tgagatcgag cattgcgccg tcgcacaccg gaagcaattt
    11101 ttcccagccg gtttcgctca acatgccatt actgtccacc agacaggtga ggtggcgcag
    11161 ttgcggatcg tttttgatag cagtaaacag cgccaccaca aacggtagct gggtcgtggc
    11221 ttcgccgccg cttaccgtta tcccttcgat aaacagcact gctttgcgga tatggctaag
    11281 cacctcatcc acgctcatgg actgcgccat tggcgtggca tgttgcggac acatcttcag
    11341 gcaggtatca cattgctcgc agaccacagc gttccacacc actttgccgt cgacaatctg
    11401 caacgcctga tgcggacact gcggcacgca ctctccgcaa tcattacagc gccccatcgt
    11461 ccacggattg tgacagtttt tgcagcgcag attgcagccc tgcaaaaaca gagccagacg
    11521 actgcctggc ccgtcaacgc aggagaaggg gataatctta ctgactaaag cgcatctgct
    11581 gttcatggct tatcacgcgc ggctggcgtt ccagaatacg agtgttgcgt gcggcttctt
    11641 ctcccagcca ggtggtgttg gtgcgtgaac cttcggcgcg atatttttct aaatccgaca
    11701 aacgcaccat ataaccggta acgcgaacca gatcgttacc gctgacattg gcggtaaatt
    11761 cacgcattcc ggctttaaag gcaccgaggc aaagctgtac cagcgcctgc ggattacgtt
    11821 tgatggtttc gtcgagcgtc agaatgtcgc tgatgccgga ataataataa gcatgatgcg
    11881 gggcgacagt ttgcagatgg gtaatcggat ctggctcatc accatacggc aagcgtgcgc
    11941 caggcgtggt gccgatatcg gaactgatcc ccgactgtgc gtgtagcatg gcgcgttttt
    12001 gccagccata tttcacgggg gtattggcga caaactccgc cagttgtgcg ctgatgcgat
    12061 aacccacttc atttgcggcg gcttctttac cgtagcgcgc ggcaatccct tctttctcac
    12121 acagcaagtt aactgcttcc gccaatccgt acatgccaaa cattggcaca aaacgttcag
    12181 ggttaatcag cccttctttc accaggaagc tattctcaaa gaagtgtgat tgttgataga
    12241 ggaattcaca ccgcgcatcg atgatggcga tctgctgctg gcagtagtgc ggtagagtgc
    12301 gtgtaaagaa gtcatccagc gattcactgc gctcggcaat ggctttcagg ttaaggcgta
    12361 ccagcgtgct gccaccaccc gccagcggca gtgagttgta acagctcaca atcccgtagc
    12421 ccccttttgt gaaaatttta tcatgcaccg gaccgttggc gatgtgcggt ttgctacatt
    12481 cacagatgtt cttcgccact tccagcagta ggtcatcagg ggtgatttca ggatcgtaga
    12541 taaaggtcag gttcggtgaa acctgcttca actctgcgtc tgcacgtaag atcgcgcggg
    12601 taatgggcga atcagacggg ccgatattgg cgtgcataaa ggcgtctggc agggttctgt
    12661 cgaggtaacg ccagaaacgt tttattcgaa tatcgatctc gtcttgtgtt agaattctaa
    12721 catacggttg caacaacgca tccagttgcc ccaggtagac cggcatcgat gtgaccgatg
    12781 gtacgtggtg gtaaagaatg gtcagcagag agagtgcatc atcaagatct ttcgcgcctt
    12841 ccagctccag ccattcggaa ccgttcgcca gaaaacgggc gtaatcgggt aagacatagc
    12901 gcggtttgta cggcgcatga ccttcaaaca tatcgcagat tacaccttca tccagcgcgc
    12961 ggcgggcttc ggcaggaagc tgtgggtaag gcagattgtt ttctgcttcc agtgccagaa
    13021 aatggcgctt ctgctccggg ctaagtaccg ggctggtgac aatttgctgg caacgttgtt
    13081 gcagtgcatt ttcatgagaa gtgggcatct tcttttcctt ttatgccgaa ggtgatgcgc
    13141 cattgtaaga agtttcgtga tgttcacttt gatcctgatg cgtttgccac cactgacgca
    13201 ttcatttgaa agtgaattat ttgaaccaga tcgcattaca gtgatgcaaa cttgtaagta
    13261 gatttcctta attgtgatgt atatcgaagt gtgttgcgga gtagatgtta gaatactaac
    13321 aaactcgcaa ggtgaatttt attggcgaca agccaggaga atgaaatgac tgatctgaaa
    13381 gcaagcagcc tgcgtgcact gaaattgatg gacctgacca ccctgaatga cgacgacacc
    13441 gacgagaaag taattgctct gtgtcatcag gccaaaactc cggtcggcaa taccgccgct
    13501 atctgtatct atcctcgctt tatcccaatt gctcgcaaaa cgctgaaaga gcagggtacc
    13561 ccggaaatcc gtatcgctac ggtaaccaac ttcccacacg gtaacgacga catcgaaatc
    13621 gcgctggcag aaacccgtgc ggcaatcgcc tacggtgctg atgaagttga cgttgtgttc
    13681 ccgtaccgcg cgctgatggc gggtaacgag caggttggtt ttgacctggt gaaagcctgt
    13741 aaagaggctt gcgcggcagc gaatgtactg ctgaaagtga tcatcgaaac cggcgaacta
    13801 aaagacgaag cgctgatccg taaagcgtct gaaatctcca tcaaagcggg tgcggacttc
    13861 atcaaaacct ctaccggtaa agtggctgtg aacgcgacgc cggaaagcgc gcgcatcatg
    13921 atggaagtga tccgtgatat gggcgtagaa aaaaccgttg gtttcaaacc ggcgggcggc
    13981 gtgcgtactg cggaagatgc gcagaaatat ctcgccatcg ccgatgaact gttcggtgct
    14041 gactgggctg atgcgcgtca ctaccgcttt ggtgcttcca gcctgctggc aagcctgctg
    14101 aaagctctgg gccacggcga cggtaagagc gccagcagct actaaggctt gatttgccgg
    14161 atgatgcagc gatgctcatc cggttccccg attacctcgt ctcttttcca cgggaggtta
    14221 ccttgtttct cgcacaagaa attattcgta aaaaacgtga tggtcatgcg ctgagcgatg
    14281 aagaaattcg tttctttatc aacggtattc gcgacaacac tatctccgaa gggcagattg
    14341 ccgccctcgc gatgaccatt ttcttccacg atatgacaat gcctgagcgt gtctcgctga
    14401 ccatggctat gcgagattca ggaaccgttc tcgactggaa aagcctgcat ctgaatggcc
    14461 cgattgttga taaacactcg accggcggcg tcggcgatgt cacttcgctg atgttggggc
    14521 cgatggtcgc agcctgcggc ggctatattc cgatgatctc cggtcgcggc ctcggtcata
    14581 ctggcggtac gctcgacaaa ctggaatcca tccctggctt cgacattttc ccggatgaca
    14641 accgtttccg cgaaattatt aaagacgtcg gcgtggcgat tatcggccag accagctcac
    14701 tggctccggc ggataaacgt ttctacgcga cccgtgatat taccgcaacc gtggactcca
    14761 tcccgctgat cactgcctct attctggcga agaaactggc ggaaggtctg gacgcgctgg
    14821 tgatggacgt gaaagtaggt agcggcgcgt ttatgccgac ctacgaactc tctgaagccc
    14881 ttgccgaagc gattgttggc gtggctaacg gcgctggcgt gcgcaccacc gcgctgctca
    14941 ccgatatgaa tcaggtactg gcctccagtg caggtaacgc ggttgaagtt cgtgaagcgg
    15001 tgcagttcct gacgggtgaa tatcgtaacc cgcgtctgtt tgatgtcacg atggcactgt
    15061 gcgtggagat gctgatctcc ggcaaactgg cgaaaaatga cgccgaagcg cgcgcgaaat
    15121 tgcaggcggt gctggacaac ggtaaagcgg cagaagtctt tggtcgtatg gtcgcggcac
    15181 aaaaaggccc gaccgacttc gttgagaact acgcgaagta tctgccgaca gcgatgctga
    15241 cgaaagcagt ctatgctgat accgaagggt ttgtcagtga aatggatacc cgcgcactgg
    15301 ggatggcagt ggttgcaatg ggcggcggac gccgtcaggc atctgacacc atcgattaca
    15361 gcgtcggctt tactgatatg gcgcgtctgg gcgaccaggt agacggtcag cgtccgctgg
    15421 cagttatcca cgcgaaagac gaaaacagct ggcaggaagc ggcgaaagcg gtgaaagcgg
    15481 caattaaact tgccgataaa gcaccggaaa gcacaccaac tgtctatcgc cgtatcagcg
    15541 aataacggta tactgatctg atcatttaaa tttgaagcac tgagtacgga gaacatatga
    15601 aacgtgcatt tattatggtg ctggactcat tcggcatcgg cgctacagaa gatgcagaac
    15661 gctttggtga cgtcggggct gacaccctgg gtcatatcgc agaagcttgt gccaaaggcg
    15721 aagctgacca cggtcgtaaa ggcccgctca atctgccaaa tctgacccgt cttgggctgg
    15781 cgaaagcaca cgaaggttct accggtttca ttccggcggg aatggatggc aacgctgaag
    15841 ttatcggcgc gtacgcatgg gcgcacgaaa tgtcatccgg taaagacacc ccgtctggtc
    15901 actgggaaat cgccggtgtc ccggttctgt ttgagtgggg atatttctcc gatcacgaaa
    15961 acagcttccc gcaagagctg ctggataaac tggttgaacg cgctaatctg ccgggttacc
    16021 tcggtaactg ccactcttcc ggtacggtca ttctggatca actgggcgaa gagcacatga
    16081 aaaccggcaa gccgattttc tatacctccg ctgactccgt gttccagatt gcttgccacg
    16141 aagaaacctt tggtctggat aaactctacg aactgtgcga aatcgcccgt gaagagctga
    16201 ccaacggcgg ctacaacatt ggtcgtgtta tcgctcgtcc gtttatcggc gacaaagccg
    16261 gtaacttcca gcgtaccggt aaccgtcacg acctggctgt tgagccgcca gcaccgaccg
    16321 tgctgcagaa actggttgat gaaaaacacg gccaggtggt ttcggtcggt aaaattgcag
    16381 acatctatgc caactgcggt atcaccaaaa aagtgaaagc gaccggcctg gacgcgctgt
    16441 ttgacgccac catcaaagag atgaaagaag cgggtgataa caccatcgtc ttcaccaact
    16501 tcgttgactt cgactcttcc tggggccacc gtcgcgacgt cgccggttat gctgcgggtc
    16561 tggaactgtt cgaccgccgt ctgccggagc tgatgtcttt gctgcgcgat gacgatattc
    16621 tgattctcac tgctgaccac ggttgtgacc cgacctggac cggtactgac cacacgcgtg
    16681 aacacattcc ggtactggtt tacggcccga aagtgaaacc aggttcgctg gggcatcgtg
    16741 aaaccttcgc ggatatcggc cagactctgg caaaatattt tggtacttct gatatggaat
    16801 atggcaaagc catgttctga tggatttggg cggagcagtg actccgcctt taatatgtca
    16861 caaaaaggat aaaacgatgg ctactccaca cattaatgca gaaatgggcg attttgctga
    16921 cgtagttttg atgccaggcg acccgctgcg tgcgaagtat attgctgaaa ctttccttga
    16981 agatgcccgt gaagtgaaca acgttcgcgg tatgctgggc ttcaccggta cttataaagg
    17041 ccgcaaaatt tccgtaatgg gtcacggtat gggtatcccg tcctgctcca tctacaccaa
    17101 agaactgatc accgatttcg gcgtgaagaa aattatccgc gtaggttcct gtggtgctgt
    17161 tctgccgcac gtaaaactgc gtgacgtcgt tatcggtatg ggtgcctgca ccgattccaa
    17221 agttaaccgc atccgtttta aagaccatga ctttgccgct atcgctgact tcgacatggt
    17281 gcgtaacgcg gtagacgcgg caaaagcact gggcgttgat gctcgcgttg gtaacctgtt
    17341 ctccgctgac ctgttctact ctccggacgg cgaaatgttc gacgtgatgg aaaaatacgg
    17401 catcctcggc gtggaaatgg aagcggctgg tatctacggc gtcgctgcag aatttggcgc
    17461 gaaagccctg accatctgca ccgtgtctga ccacatccgc actcacgagc agaccactgc
    17521 cgctgagcgt cagaccacct tcaacgacat gatcaaaatc gcgctggaat ccgttctgct
    17581 gggcgataaa gagtaattgt gttttgctga aaggcgattg tcgtgtgaag ccggagtggg
    17641 agactgctcc ggctttttag
//
LOCUS       DAZUCC010000096        17607 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZUCC010000096 DAZUCC010000000
VERSION     DAZUCC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.096"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(52..2499)
                     /gene="glgP"
                     /locus_tag="ACHY09_002753"
     CDS             complement(52..2499)
                     /gene="glgP"
                     /locus_tag="ACHY09_002753"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFS3885707.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2518..3951)
                     /gene="glgA"
                     /locus_tag="ACHY09_002754"
     CDS             complement(2518..3951)
                     /gene="glgA"
                     /locus_tag="ACHY09_002754"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFS3885708.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3951..5246)
                     /gene="glgC"
                     /locus_tag="ACHY09_002755"
     CDS             complement(3951..5246)
                     /gene="glgC"
                     /locus_tag="ACHY09_002755"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFS3885709.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5264..7237)
                     /gene="glgX"
                     /locus_tag="ACHY09_002756"
     CDS             complement(5264..7237)
                     /gene="glgX"
                     /locus_tag="ACHY09_002756"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFS3885710.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7234..9420)
                     /gene="glgB"
                     /locus_tag="ACHY09_002757"
     CDS             complement(7234..9420)
                     /gene="glgB"
                     /locus_tag="ACHY09_002757"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFS3885711.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVRTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNVGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9693..10796)
                     /gene="asd"
                     /locus_tag="ACHY09_002758"
     CDS             complement(9693..10796)
                     /gene="asd"
                     /locus_tag="ACHY09_002758"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS3885712.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10988..11581
                     /gene="yhgN"
                     /locus_tag="ACHY09_002759"
     CDS             10988..11581
                     /gene="yhgN"
                     /locus_tag="ACHY09_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFS3885713.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11638..12978)
                     /gene="gntU"
                     /locus_tag="ACHY09_002760"
     CDS             complement(11638..12978)
                     /gene="gntU"
                     /locus_tag="ACHY09_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFS3885714.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12982..13509)
                     /gene="gntK"
                     /locus_tag="ACHY09_002761"
     CDS             complement(12982..13509)
                     /gene="gntK"
                     /locus_tag="ACHY09_002761"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS3885715.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13648..14643)
                     /gene="gntR"
                     /locus_tag="ACHY09_002762"
     CDS             complement(13648..14643)
                     /gene="gntR"
                     /locus_tag="ACHY09_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFS3885716.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14867..15562)
                     /gene="yhhW"
                     /locus_tag="ACHY09_002763"
     CDS             complement(14867..15562)
                     /gene="yhhW"
                     /locus_tag="ACHY09_002763"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFS3885717.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15685..16722)
                     /locus_tag="ACHY09_002764"
     CDS             complement(15685..16722)
                     /locus_tag="ACHY09_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS3885718.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            17056..17544
                     /gene="yhhY"
                     /locus_tag="ACHY09_002765"
     CDS             17056..17544
                     /gene="yhhY"
                     /locus_tag="ACHY09_002765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFS3885719.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         4319 a         4800 c         4410 g         4078 t
ORIGIN      
        1 tccactctgt attacttcag cggaacacac ggtatttagc catatttgaa cttacaatct
       61 caccggatca atatgccaga tatgatcggc gtactctttg atcgtacggt cagaagagaa
      121 gtagcccata ttggcaatgt tcagcatcgc ttttgcggtc cactcttcct gacgctcgta
      181 gagttcgtcg actttatcct gacaatcgac atagctgcga taatccgcca gtacctggta
      241 gtgatcgccg aagttgatca gcgaatcgac cagatcgcga tagcgacccg gatcttccgg
      301 actgaataca ccgctgccga tttgcgtcag cacctgatgc agctcctcat ctttctcgta
      361 gtattcacgc ggtttgtagc cctgacgacg cagttcttcc acttcttccg ctgtgttacc
      421 aaagataaag atattgtcag caccgacatg atccagcatc tcgacattcg caccgtccag
      481 cgtaccgata gtcagcgcgc cgttaagcgc aaacttcatg ttactggtgc cggaagcttc
      541 cgtccccgcc agcgaaatct gttcagacag atcggccgcc ggaatgatca actgcgccag
      601 gctaacactg tagttcggga tgaacacgac tttcagcttg tcgccaatct gcggatcgtt
      661 gttgatcact ttcgctacgt cattgatcaa atgaataatg tgcttcgcca tgtaataggc
      721 cgaagccgcc ttaccaccaa aaatattcac gcgcggtacc cacttcgcat ccgggtcggc
      781 cttgatgcgg ttatagcggg tgatcacgtg caacacattc atcaattgac gtttgtattc
      841 gtgaatacgt ttgatttgca catcgaacag cgctttcgga tttaccacca cattcagctg
      901 ctgggcgata tactccgcca gacgcttttt gttctccagc ttcgcctgat gcaccgcgtg
      961 attaaccatt gggaaatcac agtgttgttg cagatcatta agcaggctaa ggtcggtgcg
     1021 ccagttgcgg cccaggtgtt cgtccaacac ggctgaaagc gatgggttcg ctaccgccag
     1081 ccagcgacgc ggcgtcacac cgttggtgac gttggtgaaa cgacccggga agattttcgc
     1141 aaagtcggca aatagcgatt gcaccatcag gttagagtgc agctccgata caccgttaac
     1201 tttgtggctc accacaaccg ccagccaggc catacgcaca cgacgaccgt tggattcatc
     1261 aatgatcgac gcccgtccca gcagatcggt atcgttcgga tactgttcct gcaaggtttt
     1321 caggaaatag tcgttgattt caaagatgat ctgcaggtga cgcggcagaa ttttacccag
     1381 catatcgact ggccaggttt ccagcgcctc gctcattagc gtgtggttgg tgtaggagaa
     1441 gacctgacaa cacacttcaa acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag
     1501 acgcatcatc tcaggaatcg acagcaccgg atgagtatcg ttgaggtgaa tcgcaatttt
     1561 atccgccagg ttatcgtagg ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat
     1621 ggtcgaggaa accaggaagt attcctgacg cagtcgcagc tcacgcccgg agtaggtgga
     1681 gtcgtccgga tacagtacgc gagatacgtt ctcggagtgg tttttatctt ccactgccgc
     1741 gaagtagtcg ccctggttga atttaccgag gttaatttcg ctactggctt gggcactcca
     1801 caaacgcaac gtgttggtcg cgtcggtgtc gtaacctggg attatctgat cgtaagctac
     1861 tcccagaatc tcttcggttt caatccagcg cgttttttta ccttcctgct gaatgcgacc
     1921 gccaaaacgg actttatagc gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc
     1981 cagccagtag tcaggcgact ctttctggct accgttaacg atgttctgct tgaacatacc
     2041 gtagtcatag cggatgccgt aaccgcgccc cggcaaccct aacgtcgcca gagaatcgag
     2101 gaagcaagcc gccagacggc ccaggccacc gttaccgagg cccgggtcat tttcttcatc
     2161 aatcagctct tcgagattta atcccatcgc ttccagtgcg ccctgtacat cttcgtaaat
     2221 tcctagcgac aacatggcgt tggaaagcgt acggccaatc aaaaactcca tcgacaggta
     2281 gtaaacctga cgagtttctt gcgacaactg ggcacggttt gagcgtaacc agcgctccac
     2341 gagacgatcg cgcacagcaa ataacgttgc gttcagccat tcatgtttat tggcgacgac
     2401 cggatccttg ccaatcgtaa acatcagctt gtaagcgata gagtgcttaa gagcttctac
     2461 gctaagcgtg ggcgatgaat atgtaaacgg agcattcata taggcgtttc ctgaaaacta
     2521 tttcaagcga tagtaaagct cacggtacga cttcgccgcg acctgccagc taaaatccat
     2581 tgccatagcc tgacgttgca caaaccgcca cagtgaagga cgggaccaca gtacaaaagc
     2641 acgtcgaata gcccgtaaca gcgaccaggc attactatct tcaaagacaa acccactggc
     2701 gacgccatct gcaaggttct caagagaaca gtcagaaacc gtatcagcaa gcccaccggt
     2761 gcgccgcact aacggcagcg taccgtactt caatccataa agttgcgtta agccgcacgg
     2821 ttcaaaacgg ctgggcacca gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc
     2881 ttcgtgatag ccaatctgaa cgcccacctg gccggggtat tccgctgccg ccgcaaggaa
     2941 accttcctgc agcaccggat cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag
     3001 aagaccaggt aaggcttcca gcaccaggtc gagacctttc tggctagtca gacggctcac
     3061 cactgcaaaa agcggcactt tatcgtcaac cttaagcccc attgcgattt gtaactggcg
     3121 cttattttcc gctttatctt ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc
     3181 cgtctctgga ctccagattt tctcgtccac gccgttcagt acgccggaaa gacgcccttc
     3241 acggtgacgc tgttgcaaca gaccttccat accgtaggca aactgcggtt cggtgatctc
     3301 gcgagcgtag gttgggctga ctgccgtaat gtgatcggca tagtacagac cggccttcag
     3361 aaaagagatt tgtccgttga attccagccc atgaatatta aagaatgacc atggcaattg
     3421 gatgtcattc atgtgatgtg cataaaacat gccttgatag gccaggttgt gcacagtaaa
     3481 caccgacttc gccggacgcc cgcgcgccgc cagatacgca ggcgcaaggc ctgcatgcca
     3541 gtcgtgcgca tgcaccacat caggacgcca gaatgggtca agcccgctgg ccatttctgc
     3601 cccaacccac cccagcagcg caaaacgcaa tacgttgtcg gtataggcaa ataagttggt
     3661 atcgtgatac gggcttcccg gacgatcata gagatgcggc gcgtcaatca ggtatatgcc
     3721 aaccccgttg taatgaccga acaacagcgt gatatgtccg gcgaaggtat cacgacggga
     3781 tactacctgc gcatcggtca cgccacggcg aatatcggga aatgcaggca acagtacgcg
     3841 agcgtcaacg ccgtctgcga tttgtgctgc gggtaatgcc ccaataacat cagccagacc
     3901 gccggtttta agcagcggga acatctctga acatacatgt aaaacctgca ttatcgctcc
     3961 tgtttatgcc ctaacttccg tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa
     4021 cgatagaaac gacgtgcatc ttcctctgcg ttttcaccaa tcaccatgcc ttccggaata
     4081 acacaagcac gatcgatgac gcagcggcgc agacggcacg agcgacctac ccatacttcc
     4141 ggtaacaata cggcggaatc aatgttgcag aatgaattca cgcgaacgcg cgagaacaga
     4201 acggactgca ccaccaccga accggagatc acacaaccgc cggataccag tgagttaagg
     4261 gtcatcccat ggctaccgga gcgatcctgc acgaatttcg ctggcggtaa tgattcattg
     4321 taggtgcgaa ttggccaatt gcgatcgtac atatccagtt ccggcaccac agaggccaga
     4381 tcgaggttcg ctttccagta agcttccagc gtacccacat cgcgccagta cggctcggca
     4441 tccgggtcgg attgtacgca agagagcggg aacgggtgcg cataggccag accggcttcg
     4501 gtgatcttgg gaatcaaatc tttgccaaag tcgtggctgg agttctcatc gcgatcgtct
     4561 tcttccagca gttcatacag atagtcggcg tcaaagacgt agatacccat actcgccaga
     4621 gatttgctcg gatcgttcgg cattgacggt gggttagcag gtttttcaac gaattcgata
     4681 attttatcgt tctcatcaac cgccataacg ccaaatgcgg aggcttcttc aatcggtact
     4741 ggcatacaag caacggtgca acgtgcgcct ttttcgacgt ggtcgataag catacgcgag
     4801 tagtcttgct tgtagatatg gtcgcccgcc aggatcacca cgtattccgc tttataacgg
     4861 cggataatat cgaggttttg ggtaactgca tctgcggtgc cgcgatacca gttttcccct
     4921 ttcattctct gctgtgctgg cagcagatcg acaaactcgt tcatttcttc attgaagaat
     4981 gaccagccgc gctgaatgtg ctgcaccaga gtgtgggact ggtactgggt gatcacgccc
     5041 atacgacgga tcccggagtt gatgcagtta gacagcgcaa agtcgataat gcggaactta
     5101 ccgccgaagt gtacggccgg ttttgctcgc ttattggtta aatccttcag gcgggtacca
     5161 cgtcctcccg ccagtatcag ggcaacagat ttcaatggca gctggcgcgc caacattaag
     5221 tgatcgttct tctctaaact aaccatgact aactcctttt ttatcatctc tggaacacac
     5281 acaatccgtg tgcaggtccc tgccagacag ccgtaatcac tgggttatcc tctccagcga
     5341 atgggggaat ggcgtgccac tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg
     5401 cgttaattgc gatcaaaaag cgatccgaga gcagaatttg cagctgtttc ggcccgtttt
     5461 gccactcatc cgtgcttaaa ggttgagcat atcgatttag ccaacggaca ttgccgtcgc
     5521 cttcttccca ccagcgattc tccaccaaag cgggaatgcg cttgcgcaga tggattaacg
     5581 cggcggtaaa tgcggttaaa ccactgcttg cctgcgacca gtccaaccag gttaattggt
     5641 tatcctgaca gtaggcattg ttattgccat gctggctgtg accatgttcg tcaccggcca
     5701 gtaacatcgg cgtaccctgg gagagcaaca acgttgttaa cagggcgtga atgctgtcgc
     5761 gacgccgttc aaccaggtca agagtaccgc ctaacccttc tttaccatga ttgttactgt
     5821 aattgttgtt ggtcccgtcg cgattttctt ctccgtttgc ttcattgtgt ttatggttga
     5881 agcaaacgca gtcgcgaagc gtaaaaccgt catgcgcggt gacgagatta atcgcggcac
     5941 tcggcagacg accattacgt ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg
     6001 cccccagagg caaatcataa tgaagccaga aacgacgggc agcatcgcgg aaatgatcgt
     6061 tccactcggc aaacagcggc gggaaatttc ccacctgata accaccagga gcaatatccc
     6121 acggttcagc aattaacttc acctgcgaga gcaccgggca gttctggata gcggtaaaca
     6181 acggcgcatc ctgacggaac tctggcgtac ggcccatgac tgccgccaga tcaaagcgga
     6241 aaccatcgac gtggcaggtt tctacccaat aacgcaggca ggcgctggca taatccacca
     6301 ccgccggatg actcaaattg agcgtgttgc cacaaccggt ccagttgtga taatcgccgt
     6361 cttctcttat ccaataatag ctacggttgt cgatcccacg cagagagaat aacgggccgt
     6421 cgaggtccag ttccgcacta tggttgagca cgatatcaag aatgacttcg atacccgctt
     6481 tatgcagtgc tttgattgca tcgcgaaact catccagcgc cgtttctggg gagcaggcat
     6541 acgccggatg cagcgcaaac atcgccaccg ggttataacc ccagtagtta cttagaccca
     6601 tacgttgcag gcgtggttcg ctggcaaact gcgccaccgg cagcagttcc agcgcggtaa
     6661 tgcccaactg tttcaaatag ttgatcatca ccggatgccc cagggcttta taagtgccac
     6721 ggatctcgac cgggatctcc gggtgcaggt acgttaatcc tttgacatgg gcttcataaa
     6781 tgatggtact gccccacggc gtgcgcggcg gagcatcatc ctcccagtca tagtgatcaa
     6841 ccaccactac gcatttcggc gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac
     6901 cggcgtgcag cagcgggtta tctttaaatt ccccgtcaat ttgccgcgcg caaggatcaa
     6961 tcaacaactt cgccgggtta aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc
     7021 gataaccata acgcaaaccc ggacgcgcat ccggcagata accgtgccaa atgtcgccac
     7081 tgtgccctgg caagtcatag cgatgttcct ggccattggc gtcaaagaca cacagttcta
     7141 cccgctcggc atgagcggag aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc
     7201 cgaggggtgc gggtttgcca atggcgagtt gtgtcattct gcctcccgaa ccagccagat
     7261 agtggccagc ggtggtagtg tcaggcttaa tgaatgctga cgaccgtggc tggcaatctc
     7321 atcgctgtgt accgtgccgc cattgccgac attactgccg tgatagtgca tggaatcggt
     7381 attgaggatt tcacgccatt tgcccggctg gttaatgccg aagcgataat catgacgcgg
     7441 caccggagta aagttactgg caacgatgat ttcgttaccc tctttatcgc gacgcacaaa
     7501 gatcagcacc gagcgttctt tgtcatccac caccagccat tcaaaaccgt acgggtcaaa
     7561 atccagttca tgcatcgctt tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg
     7621 ctggacaccg tggtgccagt tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc
     7681 atggttccac tcgcggccct gggcaaattc gttccccatg aacagcagtt tcttgcccgg
     7741 gaatgcccac atccagccgt agtaggcgcg caggttagcg aatttctgcc atgcgtcgcc
     7801 cggcatccgg tcgagaatcg attttttacc gtggaccact tcatcatgcg acaacggcag
     7861 gacgaagttt tcagtgtagt tgtagagcat cccgaaggtc agtttatcgt gatgatactg
     7921 acgataaacc gggtcgagct tcatgtagtc cagggtgtca tgcatccagc cgaggttcca
     7981 cttgtaccag aagcccagac cgcccatatc ctgcggacga gaaacgccag ggaaatcggt
     8041 agactcctca gccattgtca ccgcaccgga aacctgctca ccaagaatac ggttggtatt
     8101 acgcaagaat tcaatcgctt caagattctc gcgcccgcca aattcgttcg ggatccactc
     8161 cccctcttta cggctgtagt cgcgataaat cattgacgct accgcatcga cgcgcagcgc
     8221 atcaatacca aaacgttcaa tccagtaaag cgcgttaccg acaaggaagt tactgacttc
     8281 acggcgacca tagttgtaga tcagcgtgtt ccagtcctga tgatagcctt cgcgcggatc
     8341 gctgtgttca tacaagttcg tgccatcaaa ttcggcaagc gcaaagtcat cggtcgggaa
     8401 gtggcctggc acccagtcga gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa
     8461 ataacggaag tcgtcgcgag taccaaaacg gcgggtcggt gcatacagac cggttggctg
     8521 ataaccccaa ctgccatcga agggatgctc gttaatgggc agtagttcga ggtgggtaaa
     8581 gcccatccat ttagcataag gcaccagttg atcggccagc tcgcggtagc tcaaccagaa
     8641 attgttgtcg gtgtggcgac gccaggagcc cagatgaact tcataaatag agattggcgc
     8701 atcaaactga ttcgcttttt tgcgctcttc agtccgtaca accttttccg gcagcccgca
     8761 aataagagac gcggtttccg ggcgcatttg cgcttcgaag gcataagggt cggacttcag
     8821 acgcaagttg ccattggcat caatcatctc gtatttatag agctgaccgt tatgcgcccc
     8881 agggataaac agttcccaga tgccgctctc tttacgcagg cgcatcgggt gacggcgacc
     8941 gtcccagtag ttgaattgcc caaccaccga gacccgacgg gcgtttggag cccagacaga
     9001 gaaacgcgta cctgtgacgc catccatagt atctgcatgc gcgcctaagg tttcatacgg
     9061 gcgcaggtga gtaccttcag ataatagcca ggcatccatt tcctggatta gcggaccaaa
     9121 acggtaagga tcatcaatca ggttttgctg accatgccag acaacagcca actgatagcg
     9181 gaaaaaattc ttacgtcgcg gaatgacgcc gctaaagaat ccccgtgagt cgagacactc
     9241 cagttttgcg agtttgcgcc cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg
     9301 taaaagggca cggacttcca gtcccgcggt ggttttatgc attcccagta cggaaaaagg
     9361 atccgcaaaa tggcctgcaa ttagcgcgtt aatcacgtct ctatcgatac gatcggacat
     9421 gcttgtcttc ctgttttatt gtgtcacccc atccaattgg atttttgact tctggttgtg
     9481 acattttttt gacctgaacg gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt
     9541 caggtaaggc tgtgaatact catgtattca gccaccctta aagaatagcc aatgctctat
     9601 ttaactcccg gtaaatcatg aaacatctgc gcttactcct gtattacgca ctaacagggg
     9661 cggcatcgcg ccccagattt aatgaataaa gattacgcca gttgacgaag catccgacgc
     9721 aacggctccg cggcccccca cagcagctgg tcacccacgg taaaggctga caggaactcc
     9781 ggtcccatat tcagcttacg caggcggcct accggcgtgg tcagcgtgcc ggtaacggca
     9841 gctggggtta gctcacgcat agtgatttcc cgatcgttcg gaacgacttt cgcccacgga
     9901 ttgtgtgcag ccagcagttc ttccacggtc ggaatagaca catctttttt caatttaata
     9961 gtgaatgcct ggctgtggca gcgcaatgcc ccgacacgca cacataaacc atctaccgga
    10021 attacggaag atgtgttgag gatcttgttg gtttccgcct gccctttcca ctcttcgcgg
    10081 ctctgaccgt tatcgagctg tttgtcgatc cacggaatca ggctacccgc cagcggcacg
    10141 ccaaagttat ccaccggcag ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata
    10201 tcgagaatag cagaggacgg ggtcgcgagt tcatctgcca catggccata cagatggccc
    10261 atctgggtta ataactcacg catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt
    10321 gcaacggaca cccaatcaac aagatcattg gcgaataaac cacccaacga catcaacatc
    10381 aggcttacgg tacagttacc gccaacaaaa gtcctgatgc cattatttaa tccatcggta
    10441 atgacgtcct gattgacggg gtcaagaatg atgatggcat catctttcat gcgcagagac
    10501 gatgctgcgt caatccagta accttgccat ccgctttcac gaagctttgg atagatttcg
    10561 ttggtataat cgccgccctg acaggtcaca atgatatcga gggcctttag cgcctccaga
    10621 tcaaaggcat cctgaagtgt gccagtggtt ccgccaaaag acggcgcagc ctgaccaagc
    10681 tgagaagtag aaaagaagac agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt
    10741 tgcatgagaa cggagccgac cataccgcgc cagccgataa aaccaacatt tttcataagc
    10801 gttttttcct gcaaagatgt gtgctgtata aatgtgccgg tctcctcttg gcacatcttt
    10861 caccatacaa aaagcagcca aagtcgcaag tgaaattaat caatgatagc gaagccatca
    10921 gtaatgcgac ttatcctgct ttgttagcac gcagaaagtc cgcggcaatt atcagggaat
    10981 ttgagttatg aatgaaatca tttctgcagc agttttattg atcctgatta tggatccgct
    11041 cggaaaccta cctattttca tgtccgtact gaaacatact gaaccgaaaa gacggcgggc
    11101 aatcatggtg cgagagttgc ttattgctct cctggtgatg ctggtgttcc tgtttgcggg
    11161 tgagaaaatt ctggcatttc tcagcctgcg agcagaaacc gtctccattt ctggcggcat
    11221 cattctgttt ctgatcgcca ttaagatgat tttccccagc gcttcaggaa atagcagcgg
    11281 gcttccggca ggtgaagagc cgtttatcgt gccgttggca attccgttag tcgccgggcc
    11341 gactattctc gccacgctga tgttgttgtc tcatcagtac ccgaatcaga tgggacatct
    11401 ggtgattgct ctgctgctgg cctggggcgg cacctttgtc atcctgctac agtcttcgct
    11461 atttttacgt ctgctgggcg agaaaggggt gaacgcactt gaacgcctga tgggattgat
    11521 tctggtgatg atggcaaccc agatgttcct cgacggcatt cgaatgtgga tgaaggggta
    11581 acgaggagag tttttcgaaa agagcccggt agcgtcagaa ctaccgggta aacaaactta
    11641 actcaacagc tggaacgcaa tcatcccaac gatggcaccg acagtgccga ggatggtttc
    11701 catcatggtc caggttttca gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa
    11761 ccagaaaccg gcgtcgttaa cgtggctgac aacaatcgaa ccaccggcga tacaaatcga
    11821 cagcgccgcc atttgcgcac cagagtagtt cagttgttca atgactggca tcaccagtcc
    11881 caccgccgtt aaacaggcta cggtggcaga cccctgaatg atgcgcactg cagctgccag
    11941 cacgaagcag gtgatagcaa tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg
    12001 acctacgcca gagtcaacca gcacctgttt gaacacgccg cccgcaccaa tcaccagcag
    12061 aatgatcccc gccggttgca gcgcgtgacc gcaaatctcc atcactttgt cttttggcat
    12121 gccctgacgc attgccaggc cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa
    12181 cggatgacca ataaactcga accattcgta agcggtagag ccttctggca caaaacgcgc
    12241 ggcaatggtt ttcagcccca ccagcaccag cggcaacagg atcagcgaca ggctgaatcc
    12301 gaaagatggc attttacctt cgccgagatg cggttcgctg atgtcgtcag gaatatgcag
    12361 ctcaacgtaa cggctgatga agttacccca cagcggcccg gcaataatca ttcccggaat
    12421 tgccgcacac aggccaatca ggatcatcca gccaaagtcg gcgttcatct gcgatgccag
    12481 cagcattggc gctggccccg gcaccaggaa cgcagccgct gccgccacgc ctgcaaataa
    12541 tgggattacc agcttcacca ggttcgtacc ggtatgacgc gccattgaga aagcaacgct
    12601 aatcagcaga acaatcgcca cttcaaagaa cagcggtagc gcacaaacca gccccgcaag
    12661 gccaatggca taatgcgcgc ggctgtgacc gaaggattta agcattttga cggcaatctg
    12721 atcgactgcg ccggtttcat gtaagatctt gccaaacata gctcccaggg cgacaaccac
    12781 cgccaggaag ccgagggtgc ctcccatccc tttttccatc gtcgctgcga ttttatcgag
    12841 cggcataccg gaaaaaaggc cagcccccat ggacaccacc attaaagcca ggaaagcgtg
    12901 catacgcgcc ttcatgacta aaaacagcag cagtaaaaca gaccctactg ctgttaaaac
    12961 aagcgttaat gtagtcacta cttatttgcc ttttttaata acctcaatgg tgcttgccac
    13021 aacaccttcc agcggttgat cgatatccac caccagtaca tcggtttcgt ccgcacccgg
    13081 ctcctgcagc gtttcaaact gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg
    13141 cgctttcagg cggctttcaa tcacatcaaa atcacctttc aggtagatga aagagagatt
    13201 cggattacct tcacgcagca agtcgcgata gtgttttttc aatgcagaac agacgatcag
    13261 tgacacttta ttagtgcgct gcatagcaaa cgcggcgtcg ttcagcgcct gcagccacgg
    13321 tttgcgatcg tcatcattca gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg
    13381 atggaggaaa tcgccatcaa gaaacgcggc atgaagttga tgcgccactt cacttgcgac
    13441 cgcagatttg ccgctgcccg atacgcccat caagacgtaa atgtggtgat catggttagt
    13501 cgtgctcaaa gtggtgcccc cacaatacaa gaattaaaaa tgttacgggt aactgttatc
    13561 ggtaacattg tccagccgga caatatcaga agcaatatcc atccgtgcct taagtgtata
    13621 agtgtgagct acttcaaatt tgtgggctta aatagatccg cccggtgaca aggtgaaacc
    13681 taaatctaac attttcggtg tcacagattc gccacgaata cgcgccagca ggcgttcagc
    13741 gccaatactg cccatccgct cacgcggcgt caggacgctc gccaaccgtg gctccatcac
    13801 ctggccaatg tcatgaccgt ggaaaccggc aatcgccata tcgtcaggaa ctttcaaccc
    13861 cagacgctga cattcaaacg ccgcgccgac cgccaggtca tcattagtac agaacacgcc
    13921 atccagctgc ggatattccc gccgtgcctg gcgaatcagt tcaataccgg aagaataaga
    13981 agaagattgc tcaaccatca cgctatacgg caccaggcct gcatccagca tcgcctgttc
    14041 atatcccttc tgtttgatga tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg
    14101 gcgatgcccg cgagcaataa tggctgtggt catctggcgt gccgcttcaa agttatcaaa
    14161 accgacggcg atatcgaggc aaggcgactt gctgtccatc agttccacca ccgggatccc
    14221 cgccacttca atcatcttta aggtgcgcgg cgtgtgggta cgttcggtga ggatcagtcc
    14281 gtcgatattc catgagagca tggattcgag acgttcttgc tccatttccg gtttataacc
    14341 gtagtgggcc agcatggtct gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa
    14401 tacttccgcg aaaacctggt tggtgagaga aggtaacagg acgccaatcg cccggctggt
    14461 ggcgttagag aggatatcgg gtgcgcgatt gggaatataa cccagttcat caagagcggc
    14521 ggcaatcttg ccgcgtagag cgacggaaac ctgctccggg ttacgcaaaa agcggctgac
    14581 cgtcattttg gtcacgccta cacggtcagc cacatcctga agtacgggtc ttttcttttt
    14641 catcgtcctg aaggtacaaa agagatagat tctcttagtt taacacggac gcatcagaac
    14701 cttcccgggg aaaacagtgc ttgtttacaa ttattagata tacactaaat caattgcatc
    14761 gcattgtgct aacgacgcgg caaaaccccg gaagcgtaca tcagcacgcg accggagcga
    14821 gcgaggaagc ctgcaaaaag gcaggcttca aagatgccgt tgagctttaa accggcggca
    14881 gatcgaacag taacacttcg ctgtcgctat ccgcatggat ggagattgcc tgctcatccc
    14941 agattgccag accatcgctg atcgaggctt tcacgccgtt aatggtgaca ttgcctttca
    15001 ccacctggat ccagacgcgg cgttcagcgg caatctgatg caccgactgc tcatctttca
    15061 gcaacgccca gcggtacagt tccatatcct gatgcacttt caatgaacca tcacgcgcat
    15121 ctggcgaaag caccagttgt ttgccctgca ctgcatcgaa gcgacgctgt tcataacgcg
    15181 gcgtaatgcc gttttcttcc ggcatgatcc agatctgata caggtgcaga cgctcggtgc
    15241 tgcttgggtt gtactctgag tgacgaatac ccgtacccgc actcataatc tggaactcgc
    15301 ccgccggaac ctgctcttta ttgcccatgc tgtcctggtg ctcaacagta ccttccagca
    15361 cgtaggtcaa aatttccata tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa
    15421 tcacgtcgtc gttaatcacg cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt
    15481 tggcaaaaga gaaagtatgc caggagtcca gccagccatg atttgcatga ccacgttcat
    15541 ttgctttgcg taagtagatc atttgtattc gccccctgaa tgatttcgat gggcttagtg
    15601 tggacccgat ctgcctggga tgatagaggg tgaaaattga cccctctgtt caaaaaaatt
    15661 gaactattcg aggggccatc aaacttactt cgcgagagtc actgtggagg gagaggcttg
    15721 ctcaaatccg cgttcaagga tttccagatt ggtaagaact tcagattcct tgacgtaatt
    15781 tggcgcaccg ttggtgatgg tttgatacaa cgcatcataa acgcgcccgt aatcgcccat
    15841 ctccggcttc atctcttctc tgaccgtcac accctcgtca ttgacatact ccagcacacc
    15901 gaccgaatca tccgctgcga atcccggttc gcccggcata atattagcct tgaggctggt
    15961 ttcctgctgg tcgataccgt atttaataaa cgaacctttc ttaccgtgaa cgataaattt
    16021 cggataatcg attttcacca gatggctggt tttgacgatg gctttcaagt cgccataaaa
    16081 cagttgcgct tcaaaggtgt cgtcaggatt ggctttattg cgcaggctgc ggatgtcata
    16141 agcgacgtga tccgggcgac cgaacagaga aataatctgg tccatcgtat gcacaccaag
    16201 gccatagaac gcgccatcct gcggcaaccc aggtttggtt tctgccaccg ggcggtaata
    16261 gtcaaaatgg ctttccactt caacaatctc tcccaacttg ccgctttcaa tcgctttttt
    16321 cgccgtcagg aagcaggaat caaagcgacg attctgatac ggcgtgacgg tcagcccttt
    16381 gcttttcgcc agcgcaaaca gctccttcgc ctgggcaagc gtcggggtga acggtttttc
    16441 gaccagcaca tttttcccgg cttccagcgc gcgtttcgcg tattcgaaat ggctgtccgc
    16501 gtgggtgcag acgacaacca gcttaacatc gggatcgttt agcacttcgt cgagatcgct
    16561 ggtgaaatgg atatgagagt aaatgggagc ctgttcttcc ggctttgcat ggcggcgaaa
    16621 aatatgcgcg acatgccagc tatccttgcg gttaagtaca tacggcagat ggtaacgggt
    16681 ggtgcttttg ccgaagccaa taaaggcgca gttgatgacc atgatttcgt cctttttaag
    16741 gtggttattt acaccttagc gcaaagcgga cgtggttcct actggagtgc gcataacgaa
    16801 cacaagcact tcccgaggat aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg
    16861 ctggctaagt aatactggaa gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg
    16921 gtcttcctga tcgcgagaca ataataatca ttctcattcg cacttgtcca acactttttg
    16981 caaaaaaatg catttgactc gcattcgaaa gtcaatgatg ttgaaaggga catttacccc
    17041 aaagaggaca aaggaatgag tgagatagtg atacgccacg cagaaacacg ggattacgag
    17101 gccatcaggc agattcacgc ccagccggag gtgtattgca acacactaca ggtgcctcat
    17161 ccctccgatc atatgtggca ggaacgactc gccgatcgcc ccggcatcaa gcaactcgtc
    17221 gcctgtattg atggaaccgt agtaggtcat ctcaccattg atgtacagca acgcccgcgc
    17281 cgcagtcatg ttgccgattt tggtatctgt gtcgactccc gttggaagaa ccgcggcgtc
    17341 gccagcgccc tgatgcgaga gatgattgaa atgtgcgaca actggttgcg ggtagatcgt
    17401 attgagctaa cggtgttcgt cgataacgcg ccagcaatta aggtctataa aaaatttggc
    17461 tttgagattg aagggactgg caagaagtac gcattacgta atggtgaata tgtcgatgcg
    17521 tattatatgg cgcgggtgaa gtaagatagt gccctttttc tgagatggaa aaagggtgtc
    17581 attcaaaatc ggcatacctt cctttaa
//
LOCUS       DAZUCC010000097        17449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZUCC010000097 DAZUCC010000000
VERSION     DAZUCC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.097"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(44..697)
                     /gene="fdnI"
                     /locus_tag="ACHY09_002766"
     CDS             complement(44..697)
                     /gene="fdnI"
                     /locus_tag="ACHY09_002766"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFS3885720.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(690..1574)
                     /gene="fdnH"
                     /locus_tag="ACHY09_002767"
     CDS             complement(690..1574)
                     /gene="fdnH"
                     /locus_tag="ACHY09_002767"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFS3885721.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVNLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEEHHE"
     gene            complement(1587..4634)
                     /gene="fdnG"
                     /locus_tag="ACHY09_002768"
     CDS             complement(1587..4634)
                     /gene="fdnG"
                     /locus_tag="ACHY09_002768"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4047..4049),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFS3885722.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4866..5747
                     /gene="yddG"
                     /locus_tag="ACHY09_002769"
     CDS             4866..5747
                     /gene="yddG"
                     /locus_tag="ACHY09_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFS3885723.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LSFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            6020..7408
                     /gene="narU"
                     /locus_tag="ACHY09_002770"
     CDS             6020..7408
                     /gene="narU"
                     /locus_tag="ACHY09_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFS3885724.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            7503..11243
                     /gene="narZ"
                     /locus_tag="ACHY09_002771"
     CDS             7503..11243
                     /gene="narZ"
                     /locus_tag="ACHY09_002771"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFS3885725.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELLLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVF
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGPTEGIQPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDMVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLAQHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            11240..12784
                     /gene="narH"
                     /locus_tag="ACHY09_002772"
     CDS             11240..12784
                     /gene="narH"
                     /locus_tag="ACHY09_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFS3885726.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLNVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            12784..13479
                     /gene="narW"
                     /locus_tag="ACHY09_002773"
     CDS             12784..13479
                     /gene="narW"
                     /locus_tag="ACHY09_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFS3885727.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            13476..14156
                     /gene="narI"
                     /locus_tag="ACHY09_002774"
     CDS             13476..14156
                     /gene="narI"
                     /locus_tag="ACHY09_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFS3885728.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            14236..15129
                     /locus_tag="ACHY09_002775"
     CDS             14236..15129
                     /locus_tag="ACHY09_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFS3885729.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFESPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPDKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGVAVILFHAEWAIEL"
     gene            complement(15225..16070)
                     /gene="nhoA"
                     /locus_tag="ACHY09_002776"
     CDS             complement(15225..16070)
                     /gene="nhoA"
                     /locus_tag="ACHY09_002776"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFS3885730.1"
                     /translation="MTPILNHYFARINWSGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            16245..16814
                     /locus_tag="ACHY09_002777"
     CDS             16245..16814
                     /locus_tag="ACHY09_002777"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS3885731.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFYCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(16968..17348)
                     /locus_tag="ACHY09_002778"
     CDS             complement(16968..17348)
                     /locus_tag="ACHY09_002778"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3885732.1"
                     /translation="MIIRPVKPSDVEPLLDMLLASGQFNQDGLHHVQKTLTHYFSGQS
                     ADLWFSAEHLGLAGIAYCAPEVMTNDVWNLLMHWISPSHQRQGVGHLLINEIEKELTI
                     KQARLLLVETSSYVDFSAARAFYT"
BASE COUNT         4091 a         4609 c         4458 g         4291 t
ORIGIN      
        1 gcgcttctta aagcgccatt ttcagcttaa agttcaaaag agattatatc ccttcttcac
       61 tctctttttt cgcttccgcc ttctcgattt cacgatacca gcgcggatgg tgtttcttcg
      121 cccagcgacg gctcaccttc ccttcgatca tccctttaat cgatcctttc acccagaatg
      181 ccatatacat atggatcagg atggcgtgga tcaggatgat acccgcagcc gcgtgaatca
      241 gcaggctgta gcgaacaacc tgcatcggga agtactgcgc aaagtacgga cgccagataa
      301 tcaccccggt caccagcagc acgaaaatca tgctcatgat cgaccagaac atcatcttct
      361 gcccggcgtt gtacttacca acatccgcca ctttgtgctc attgcctttt aatacttcga
      421 caatgttcaa cagccacgga atatccttct tatccgggat attgtgatgt acaaaacgca
      481 caaacataaa catcagcgcg acgaaaatcg caatgccgaa gaacgggtgc aaaatgcgtc
      541 ccatctgcgg cgtaccgaag gtttgcgtca gccattgcag cgtcgggaag aagaacgaaa
      601 tcccggacag cgccaccagg aagaagcaaa tcaccacggt ccagtgacag gcgcgatcaa
      661 taaacttggt gcgcacaatc attttcgact tactcatgat gctcctcctc gtcatcgtcc
      721 acttccttat tcgggccaat accgatgtag tggaaaatca acccggcaaa agtggcgata
      781 aagccagccg ctgccagcgg tttcaacgcg cctttccaca agttcaccga tgtgtcgatc
      841 ttcggatctt tcggcagacc gtgatacagc tccggctgat cggcgtgatg caacacgtac
      901 atgacgtgcg taccaccgac cccttccggg ttgtagacgc cagcatgttc gtaaccacgc
      961 gctttcagtt tcgctacgcg ctgttccgcc agctccagca tctccttctt ggtgccgaag
     1021 tggatagccc cggtcggaca ggttttcaca caagccggtt cctggccgac gctgacgcga
     1081 tcgacgcaga gcgtgcattt atatacccgg ttatcctctt tgttgaggcg cggaatatta
     1141 aacggacacc cggcaatgca gtaaccacag ccgatgcagt tttccgactg gaaatcaaca
     1201 atcccgttag cgtactgaat gattgcacca gcagacgggc acgccttcag gcagcccgga
     1261 tcttcacagt gcatacagcc atctttacgg atcagccact ccagcttgcc gttctgttcg
     1321 gtttcgctaa agcgcatcac cgtccaggac ttggcgctca gatcggcggg gttatcgtaa
     1381 accccgacgc agtgccccac ttcatcacgg atgtcgttcc actccgaaca cgccacctga
     1441 caggctttac agccgataca ggtggaaacg tcgataagtt tcgcgacttc tgctttgtaa
     1501 tcacgcacct gagaaggcgg cgtgatggag ttagttgcgg accttttgat aatgtcctgc
     1561 gtttccatag ccatctgttc gcccccttac gccttctcga tgtttactaa gaacgcttta
     1621 tattccggcg tttgcgagtt tgcatcaccg acattcggcg tcagagtgtt agcgatataa
     1681 cctttacgcg cgacaccctc aaagccccag tggattggaa tacccaccgt ttcaacctgc
     1741 tgaccgttta cattcagcgg tttcagacgg cgcgttacca cagccaccgc gcggataaag
     1801 ccacgcttgc tggagacagt gacacgatcg ccattggcaa tgcctttcgc cgccgccagc
     1861 gtttcgctga tttccacaaa ctgttccggc tgagcaattg cgttgagcaa tgcgtgcttg
     1921 gtccaggtgt ggaagtgctc cgtcagacga taggtcgtac ccacatacgg gaactgctct
     1981 tttttaccca tccgcagtgc gtcttgttca tacagacgaa caaccgggtt agacaccacg
     2041 ttcggatgca acgggttagt gcccagcggc gtttcaattg gctcatagtg ttccgggaac
     2101 ggaccttccg ccattttgtt gatggcaaac aggcgtccca tcccttccgg ctgcatgata
     2161 aacggcccgg ttggcgtacc cggtgcggca ttgccgaagt ccggaatatc gttacccgtc
     2221 cacttgctac cgttccactg gatcagcatc cgtttcggat cccacggttt accgttgata
     2281 tccgccgaag cacggttgta gagtacgcga cggttgagcg gccacgccca ggcccatccc
     2341 agcgtattcc ccagaccgga cgggtcggag ttatcgcggt tagccatctg gttgccctgc
     2401 tcggtccagc taccggtgta gatccagcaa gaagatgcgg ttgtaccgtc atcacgcaga
     2461 tgcgcaaagc tactcagcaa ctgacctttc ttcgcaatca gcacgccatt agcgtcatag
     2521 agatcttcca gcgcatagcc gttgttctct ttcgccactt cgtcagactg cggttcgtgc
     2581 ggctgcttgt agttccagct catcttcatc agcggttcta cgcctttacc accctcggcc
     2641 tggtacagct cgcgcagatg atggtagata cccgccagaa tttcgccgtc gttacgtgct
     2701 tcgcccggcg cgtcctgacc tttccagtgc cactgcaacc agcgaccgga gttagcgata
     2761 gaaccatctt cttcagcaaa gcaggtcgaa ggcagacgga atacttcagt ctgaatagat
     2821 gccggatcga catcgttcga ctcgccgtgg ttctgccaga aggtagaggt ttcagtcacc
     2881 agcggatcga taaccaccat gtacttcagt ttgctcaggc agctcaccac tttgtttttg
     2941 tccgggaagg acgcaaccgg gttaaagccc tggcagaaat aaccggtgac tttaccttca
     3001 tccatcatgt tgaaatactt gatgacgtcg taggtctggt cccacttcgg cagccagtcg
     3061 tagccccagt tgttctcttt ctgcgcggca tcgccgtaga aagatttcat caggctaacg
     3121 aaaaacttcg gatagttgct ccagtagttc acctgatcag ccagcgtcgc tttcggcgtg
     3181 ttcgcttcca gatacgactg caaatcaacc tgtttttctg acggcagcgt cagataacca
     3241 ggcaggctgg tagagagcag gcctaagtca gtcaggccct gaatgttgga gtgaccacgc
     3301 aatgcgttca cgccgccacc ggccataccc atgttaccga gcagcaactg gatcatcgcc
     3361 atcgtacgga tgttctgcgc acccacggtg tgctgcgtcc agcccagcgc gtacaggaag
     3421 gtggttgtgc gatccggtgc gctggtggag gccagcactt cacacacttt caggaagtcg
     3481 gcttttggcg taccacagat gttttcaaca acgtccggcg tgtagcggga aacgtgcgct
     3541 ttcagcaggt tccacacaca gcgcggatga gtcagtgttt catcgcgttt cgcatagccg
     3601 ttttcatcga actgatagtt ccaggacgat ttatcgtatt gccgtttttc agcgtcgtag
     3661 ccgctgaaca gaccgtcttc gaaagcaaaa tcatcacgca ccagcaggct ggcattggtg
     3721 taatgcttaa cgtattcggc attgattttg ttgttttcga tcaggtagcg caaaacgcca
     3781 gacaggaacg taatgtccgt accggaacga ataggtgcgt aaatatccgc tacagaagcg
     3841 gtacgcgtaa aacggggatc gacaacgatc aaggttgcat cgttgttgtt tttcgcttcc
     3901 atcgcccagc ggaaaccgac gggatgcgct tcagcagcgt taccgcccat caccattacg
     3961 acgttagcgt ttttgatatc cacccagtgg ttggtcatcg caccgcgacc aaatgttgga
     4021 gcaagacttg ctaccgttgg tccgtgtcag acgcgcgcct ggttgtctac tgccagcatc
     4081 ccgagggagc gggcaaattt ttgagtcagc atcccggttt cgttgctggc accggaggca
     4141 cacagcatac cggtagaaag ccaacggttt accgttacgc cctgctcgtt cttttcaata
     4201 aagttagcgt cacggtcagc tttcatcagc ttcgcaatac gggagaatgc ttcttcccag
     4261 ctaatgcgct gccatttgtc agaacctggc gcacgatatt ccgggtagcg cagacggttt
     4321 tcgctgttga cgtaatccag caaaccggcc cctttcgggc acagcgcacc acggcttacc
     4381 ggatgatccg ggtcaccttc aatgtgataa atcgcttctc tggcgttttt cgcgccatca
     4441 cccaggctat acatcaatag cccgcaacct acggaacagt atgtgcaggt gttacggatc
     4501 tctttcgcgc gtaataattt gtagtttcgc gcctgagcca gtgcttgctt cggggcaaag
     4561 cccaatgccg ctaccgttgt tccagccata ccgcccgcgc agattttaaa aaattgtctg
     4621 cgactgacgt ccattgcttt cctctttttt caggggtatt actgcgcgag gaaactaaca
     4681 gtaaaattct gaatcttttt gcgcgaaatc aaaaagaaca cgtgtttgcc acaaaataat
     4741 ccgcctgcgc aaattgacct acctcaatag cggtagaaaa acgcaccact gcctgacagg
     4801 ccagttaaaa aaatgctata aaattcagct taatttttaa cggcaagaga gacaaaacag
     4861 cgagcatgac acgacaaaaa gcaacgctca tagggctaat agcgatcgtc ctgtggagca
     4921 cgatggtagg attgattcgc ggtgtcagtg aggggctcgg cccggtcggc ggcgcagctg
     4981 ctatctattc attaagcggg ctgctgttaa tcttcacggt tggatttccg cgtattcggc
     5041 agatcccgaa aggctattta ctcgccggga gtctgttatt cgtcagctat gaaatctgtc
     5101 tggcgctttc cttagggtat gcggcgaccc gtcatcaggc gattgaagtg gggatggtga
     5161 actatctatg gcccagcctg acaattctct ttgccattct gtttaatggt cagaaaacca
     5221 actggctgat tgtaccagga ttattattag ccctggtcgg cgtctgctgg gtgttaggtg
     5281 gtgacaatgg gttacattat gatgaaatca tcaataatat caccaccagc ccattgagtt
     5341 atttcctgtc gtttattggt gcgtttatct gggcagccta ctgcacagta acgaataaat
     5401 acgcacgcgg atttaacgga attaccgttt ttgtcctgct aacgggagca agtctgtggg
     5461 tttactattt tctcacgcca caaccagaaa tggtatttag cacgccagtc atgattaaac
     5521 tcatctctgc ggcatttacc ttaggatttg cttatgctgc atggaacgtc ggtatattac
     5581 atggcaacgt caccattatg gcggtaggtt cgtattttac gcccgtcctt tcctcagcgc
     5641 ttgcagccgt gctgctcagc gccccgctgt cgttctcgtt ctggcaaggc gcgctaatgg
     5701 tctgcggcgg ttccctgctc tgctggctgg cgacacgtcg tggctaaaat aattgtcggg
     5761 tctacggccc ggcagttaat cagataaatt ctgacaatat taaaaaataa atttcaaata
     5821 ccagaaagcc attttagtta catttagtaa cacatatctg tctgtttaat ccacattctg
     5881 cccccgcagt tacgctttct tatctattct tctaatcagc acttcacctg attcatcctt
     5941 aattctaata actggctatt gcgccttacc tcctgttttg tctttttttc attaccaatg
     6001 tgtgcatgtg aggaacaata tggcactgca aaatgagaaa aatagtcgtt atcttttacg
     6061 cgactggaaa ccagaaaatc cggccttctg ggaaaataaa ggaaagcata ttgcacgaag
     6121 aaacctctgg atatcagtca gttgcctact tcttgccttc tgtgtctgga tgctatttag
     6181 cgcagttact gttaatctca ataaaatcgg ttttaatttc actaccgatc aactcttttt
     6241 attaaccgca ttaccctccg tttctggcgc attattgcgt gtcccctact cctttatggt
     6301 gcctatattc ggtggacgcc gatggacggt ttttagtact gcaatcctga ttattccttg
     6361 cgtctggctc ggaattgccg tgcagaatcc gaatactcct tttgggatat ttatcgttat
     6421 cgctttgcta tgcggttttg caggtgcgaa ctttgcttcg agcatgggca atatcagttt
     6481 cttctttcca aaagccaaac aagggagcgc tcttgggatt aatggcggat taggaaactt
     6541 aggtgtaagt gtgatgcagc tggttgcacc gctggtcatt tttgtccccg tatttgcctt
     6601 tctcggcgtc aatggcgtac cgcaggccga cggttcggtg atgtcgctgg cgaatgccgc
     6661 atggatttgg gtgccattac tggcgattgc cacgatcgcc gcctggtcag ggatgaatga
     6721 tatcgccagt tcacgcgcct caattgccga ccagctccct gtcttacaac gcctgcatct
     6781 ctggctgctg agcctgcttt accttgccac cttcggttcg tttatcggtt tttctgcggg
     6841 ttttgccatg ctggcaaaaa cccagttccc ggatgtgaat attctgcgcc tggcgttctt
     6901 tggcccattt atcggtgcca tcgcgcgatc ggttggtggt gctatttccg ataaattcgg
     6961 cggcgtgcgg gtgacgttga tcaacttcat ttttatggcg attttcagcg ccctgctgtt
     7021 ccttacctta ccgggcacag gctccggtaa tttcatcgca ttttacgccg tatttatggg
     7081 gctgtttctg accgcgggtc tgggaagtgg ttctactttc cagatgatcg ccgtcatctt
     7141 tcgccagata acaatttatc gggtgaagat gaaaggcggt agtgatgagc aagctcaaag
     7201 agaagccatc accgaaacgg cagccgctct gggctttatc tcagccattg gcgcagtggg
     7261 cggctttttt attccgcagg cgtttggcat gtcgctcaat atgaccggct ctccggtggg
     7321 cgcgatgaaa gtgtttttaa tcttctacat cgtttgtgtg ctactgacct ggctggttta
     7381 tggtcggcgg aagttcagcc aaaaataaga gatcgatgcc tgacacgtct tatcaggcta
     7441 caaccgtgtc gcatccgaca aggcatcaga tcccaattca ttcctggagc aggagttatg
     7501 tcatgagtaa acttttggat cgctttcgct acttcaaaca aaagggcgaa acctttgccg
     7561 atggtcacgg acaggtgatg catagcaacc gcgactggga ggacagctat cgccagcgtt
     7621 ggcagttcga caaaatcgtg cgttccaccc acggtgttaa ctgtacaggc tcctgtagct
     7681 ggaaaatcta cgttaaaaat ggtctggtga cctgggaaat ccaacagacc gactacccgc
     7741 gcactcgccc tgacctgcca aatcatgaac ctcgcggctg cccgcgtggc gcaagttact
     7801 cctggtatct ctacagcgct aaccgcctga aataccctct cattcgtaaa cgactgattg
     7861 aactgtggcg cgaagccctc aagcaacaca gcgatccggt actggcgtgg gcatcgatta
     7921 tgaacgatcc gcaaaagtgc ctgagctaca aacaagtacg tggacgcggc ggctttatcc
     7981 gctctaactg gcaggagcta aatcagctga ttgctgccgc taacgtctgg accatcaaaa
     8041 cctacggccc ggatcgcgtt gccggtttct cgccgatccc ggcgatgtcg atggtttctt
     8101 acgccgccgg aacacgttat ctttcactat taggtggcac ctgtttaagc ttctacgact
     8161 ggtattgcga cctgccgccc gcctcgccga tgacctgggg cgagcaaacc gacgtaccgg
     8221 aatctgccga ctggtataac tccagttaca tcatcgcctg ggggtctaac gtaccgcaga
     8281 cacgtacgcc ggacgcccat ttctttaccg aagtacgcta caaaggcact aaaaccatcg
     8341 ccattacccc tgactactct gaagtggcca aattgtgcga ccagtggctg gcaccgaaac
     8401 aaggcactga tagtgccctg gcaatggcaa tgggccatgt cattttaaaa gagtttcatc
     8461 tcgataatcc cagcgattat tttattaact actgccgccg ctacagcgac atgccgatgc
     8521 tggtaatgct ggaacctcgc gacgacggca gctacgttcc cgggcgcatg gtccgcgcat
     8581 ctgacctggt ggatggactg ggcgaaagca ataatccgca gtggaaaacc gtggcagtta
     8641 ataccgcagg tgaactggta gtgccgaacg gttcgattgg ttttcgctgg ggagaaaaag
     8701 gcaaatggaa tctggaatcc attgccgccg gtacggaaac cgaattgttg ttaactctgc
     8761 tcggtcaaca tgatgccgtt gcaggcgtgg ccttccccta ctttggcggc attgaaaatc
     8821 cgcattttcg cagcgtaaaa cacaatccgg tcctggtgcg ccaattgccc gttaaaaacc
     8881 tgacattagc cgatggcaac acctgtccgg tcgtcagcgt ttatgatttg gtactggcga
     8941 attatggcct cgatcgcggg ctggaagatg aaaacagcgc gaaagattac gctgaaatca
     9001 aaccgtacac cccagcctgg ggtgagcaaa ttaccggcgt gccgcgccag tatattgaaa
     9061 ccatcgcccg tgaatttgcc gataccgccc ataaaacgca tgggcgctcg atgattatcc
     9121 tcggtgctgg cgtcaaccat tggtatcaca tggacatgaa ctaccgtggg atgatcaaca
     9181 tgctgatctt ctgcggttgt gtcgggcaaa gcggtggcgg ctgggcacac tatgtcggtc
     9241 aggaaaaact gcgcccgcaa accggctggt tgccgctggc ctttgcgctc gactggaacc
     9301 gaccaccgcg ccagatgaac agcacctcgt ttttctacaa tcattccagc caatggcgct
     9361 atgaaaaagt ctttgctcag gagttacttt caccgctcgc cgatgccagt aagtacagcg
     9421 gtcatctgat tgatttcaac gttcgtgccg aacgtatggg ctggctacct tctgcgccgc
     9481 agctggggcg taacccgctc gggattaaag ctgaagccga caaggccgga ttatccccca
     9541 cagaatttac cgcccaggcg ctgaaatcgg gcgatttacg catggcctgc gaacaaccag
     9601 atagcggcag caatcatccg cgtaatttgt ttgtctggcg ttctaacctg cttggctcct
     9661 ccggcaaagg ccacgagtat atgcagaagt atctgctggg tacggaaagt ggcattcagg
     9721 gggaagaact tggaccgacg gagggaatcc aaccggaaga agttgagtgg caaactgcag
     9781 cgattgaagg caagctcgac ctgctggtga cgcttgactt ccgcatgtcc agcacctgcc
     9841 tgttctccga tatcgttctg cccaccgcca cctggtacga aaaagacgat atgaatacct
     9901 cggatatgca tccgtttatt catccgcttt ctgcggcggt cgatccggcg tgggaatcac
     9961 gcagcgactg ggaaatctac aaaggtatcg ctaaagcctt ttcgcaagtg tgcgtggggc
    10021 atctcggcaa agaaaccgac atggtattac aaccactgct gcatgactcc ccggcagagc
    10081 tttcacagcc gtgtgaagtg ctcgactggc gcaaaggtga atgcgatctg attccaggca
    10141 aaaccgcgcc gaatattgtg gcggtggagc gcgactaccc tgctacctat gaacgcttta
    10201 cctcactcgg gccattgatg gacaaacttg gcaacggcgg taaagggatt tcgtggaata
    10261 cccaggatga aatcgatttc ctcggtaaac tcaattacac caagcgtgat ggcccggcac
    10321 aggggcgtcc gctgattgat accgcgattg acgcatcaga agtgattctg gcgctggcac
    10381 cggaaaccaa cggccatgtt gcagtcaaag cgtggcaagc gctgggcgag atcaccggac
    10441 gcgaacatac ccatctggcg cagcacaaag aggacgagaa gattcgcttt cgcgatattc
    10501 aggcgcagcc gcgtaaaatt atctccagcc ccacatggtc tggtctggaa agcgatcacg
    10561 tttcctataa cgcgggatac accaacgttc atgagttaat tccgtggcgc acgctgtcgg
    10621 gacgccagca actctatcag gatcatccgt ggatgcgtgc ttttggtgaa agtctggtgg
    10681 cttatcgccc gcctatcgac acccgcagtg tcagtgaaat gcgccagata ccgccaaacg
    10741 gcttcccgga gaaagccctt aacttcctga caccgcacca gaaatggggc attcactcaa
    10801 cctacagtga aaacctgcta atgctgacgc tctctcgcgg tggaccgatt gtctggatca
    10861 gcgaaaccga tgcccgtgaa ctgaccattg tcgataacga ctgggtggaa gtgtttaacg
    10921 ccaatggcgc gctgacggcc cgcgcggtgg tcagccagcg tgtaccaccg ggcatgacca
    10981 tgatgtatca cgctcaggaa cgcattatga atattccagg ttcggaagta accggcatgc
    11041 gcggcggcat tcataactcg gtcacccgcg tttgtccgaa accaacgcat atgattggcg
    11101 gttacgcgca gctggcctgg ggctttaatt actacggcac cgtcggctcg aaccgtgacg
    11161 aattcatcat gatccgcaag atgaagaacg ttaactggct ggatgatgaa ggtcgcgatc
    11221 aggtacagga ggcgaaaaaa tgaaaatacg ttcgcaagtc ggcatggtgc ttaacctcga
    11281 taaatgtatc ggctgccata cctgttcggt gacctgtaaa aacgtctgga ccggacgcga
    11341 aggcatggag tacgcatggt ttaacaacgt cgaaaccaaa ccgggcattg gttatccgaa
    11401 aaactgggaa gatcaggaag aatggcaagg cggctgggtg cgtgatgtga atggcaagat
    11461 ccgcccgcgt ctgggcagca agatgggcgt aataaccaaa atcttcgcca acccggtggt
    11521 gccgcagatt gatgattact acgaaccttt caccttcgac tacgaacatt tgcatagcgc
    11581 accggaaggc aaacatatcc ctactgctcg cccgcgttca ctgattgacg gcaaacggat
    11641 ggacaaagtg atctgggggc caaactggga agaactgctg ggcggcgaat ttgaaaaacg
    11701 cgctcgcgac cgcaacttcg aggccatgca aaaggagatg tacgggcagt ttgaaaacac
    11761 cttcatgatg tacctgccgc gcctgtgcga acactgcctc aatcccagct gcgtggcgac
    11821 ctgcccaagc ggtgctatct acaaacgtga agaagacggc attgtgctga ttgatcagga
    11881 taaatgccgt ggctggcgtt tgtgcattag cggttgtccg tacaaaaaaa tctacttcaa
    11941 ctggaaaagc ggcaagtcag aaaaatgtat cttctgttac ccacgaattg agtcaggcca
    12001 acccaccgtg tgctcagaaa cctgcgtggg tcgcatccgg tatctgggcg tgctgcttta
    12061 cgacgccgac cgcatcgagg aagcggcgag caccgagcgc gaagttgacc tctatgaacg
    12121 tcagtgcgaa gtgttcctcg atccgcacga tccctcagtg atcgaagaag ccctgaaaca
    12181 aggtattcca caaaacgtga ttgaagctgc ccagcgttcg ccagtctaca aaatggcgat
    12241 ggactggaaa ctggcgttac cgctgcaccc tgaataccgc accctgccga tggtctggta
    12301 cgttcctccg ctgtcaccga ttcagtctta cgctgatgcg ggcggtttgc cgaaaagcga
    12361 aggcgtgctg cctgctatcg aaagcctgcg tattccggtg caatatctcg ccaatatgtt
    12421 gagtgctggc gataccggtc cggtactgcg ggcgctgaaa cggatgatgg cgatgcgcca
    12481 ctatatgcgt tcacaaaccg tggaaggcgt taccgatact cgtgccatcg acgaagtagg
    12541 cctgaacgtc gcccaggtcg aagagatgta tcgctacctc gccattgcca actatgaaga
    12601 ccgttttgtc attccaacca gtcatcggga aatggcgggc gatgcctttg ctgaacgcaa
    12661 cggctgcggt tttacctttg gcgacggttg ccacggctcg gacagcaaat tcaacctgtt
    12721 caacagtagc cgtatcgatg ccatcaacat caccgaagtg cgcgacaaag cggagggcga
    12781 ataatgcaga tcctcaaagt gatcggcctg ttgatggagt atccggacga gctgttgtgg
    12841 gaatgtaagg aggacgcgct ggcgttgatc cgccgcgacg caccgatgct caccgatttt
    12901 gcactcgacc tgctcaacgc gccgctgctg gataaacagg ccgaatggtg tgaagtgttt
    12961 gaccgcgggc gcaccacgtc gctgctgctg tttgaacatg ttcatgccga gtcccgcgat
    13021 cgcggccagg caatggtgga cctgctggcg gagtatgaaa aggtcggtct gcaactgaat
    13081 tgtagggaac tgcccgatta tctaccgctg tatctggagt atttaagcat acttccggac
    13141 gatcaggcga aagaaggatt actcaacgtt gcgccgatcc tcgccctgct tggcggacgc
    13201 ttaaaacaac gcgaggcacc gtggtacgcg ttatttgacg ccctgttgca actggcagga
    13261 agccctctat caagtgacag tgtcacaaaa cagattcgca gtgaagagcg tgacgacacc
    13321 cgccaggcgc tggatgcggt gtgggaagaa gaacaggtga agtttattga agataacgcc
    13381 acggcgtgtg acagctcgcc gcttaatcaa tatcagcgac gctttagcca ggatgtcgcg
    13441 ccgcagtatg tcgacatcag tgcgggaggt gggaaatgat tcagtatctg aacgtctttt
    13501 tttacgatat ctacccgtac ttgtgtggca cggtttttat ccttggaagc tggctgcgct
    13561 acgattacgg gcagtatacc tggcgtgccg cctccagcca gatgctggat aaacgcggga
    13621 tggtgctatg gtcgaacttg ttccatatcg gcattctggg gatcttcttc ggtcatctgt
    13681 tcggcatgtt aacgccgcac tggatgtacg cgtggtttct gccggtggcg gtaaaacagc
    13741 agatggcgat gatcctcggc ggaatatgcg gggtattgac gctgattggc ggtgcggggc
    13801 tactggtgcg tcgtttaaca aatccgcgta tcagggcgac gtcttctggt gcggatatcc
    13861 tgatcctcgc cattttgtta attcagtgca ttcttgggtt aaccaccatt cctttttcag
    13921 cacaacatcc ggacggcagt gaaatgatga agctggtggg ctgggcgcaa agcattgtca
    13981 ctttccaggg cggtgcatct acgcatcttg atggtgtcgc ctttatattc cgcgttcatc
    14041 tggtgcttgg aatgaccatc ttcctgctct tcccgttcac ccgactggtg catgtctgga
    14101 gcgcgccgtt tgagtacttt actcgtcggt atcagattgt gcgttcgcgg cgttaattct
    14161 cacgcgggcc ggacagccct tcgctctccg gccacttgca ggctatgata gttctccagc
    14221 cacaggagaa cattaatgaa accgcaggtt taccacgtcg atgcttttac ctctcaaccg
    14281 tttcgcggca attctgccgg tgttgttttc cccgccgata atctcagtga agcgcaaatg
    14341 cagcttatcg cccgcgagtt aggtcattcg gaaactgctt ttctgctgca cagcgacgac
    14401 agcgatgtgc gcatccgcta ctttacgcca acggttgaag ttccgatttg tggtcacgcg
    14461 acagtagctg cgcactatgt acgtgcaaag gtgttaggtt taggaaattg cacggtctgg
    14521 caaacatcgc tggcaggaaa acatcgcgtg actatcgaaa agcacaacga tgattatcgt
    14581 atttcgctgg aacaaggtac gccgggcttt gagtcaccgc tggaaggtga aacacgtgcg
    14641 gcgattatca acgcgcttca tctcactgaa gacgatattc tgccaggctt gccaattcag
    14701 gtggcaacca caggacactc aaaagtgatg atcccactga aaccggaagt ggatatcgac
    14761 gccctttcgc ctgaccttaa tgcgctgacc gctatcagca aacagattgg ctgcaatggt
    14821 ttcttcccgt tccagatccg cccagacaaa aacgaaaccg acggtcgtat gttctcgcct
    14881 gccattggta ttgtggaaga tccggtgact ggcaatgcca atggcccgat gggtgcatgg
    14941 ttagtacatc acaacgtatt gccccacgat ggcaacgtgt tgcgtgttaa aggccatcag
    15001 gggcgcgcat tagggcgtga cggcgtgatt gaagtgacgg tgacaattcg tgataaccaa
    15061 ccggagaaag tcaccatttc tggcgtggcg gtgattttgt ttcatgccga atgggcaatt
    15121 gaactctgaa accgttgcct gatgcgcttc gcttatcagg tctacaaccg ggctaatgta
    15181 ggccggataa ggcgtttacg ccacatccgg caacctgaca taaattattt tcccgcctcc
    15241 gggtgcgtat caaacgccgc catcaccagc gctaactcat ccacagtaaa gccatgtttc
    15301 gcatcatcta cgcccagacc aaactgttcc tgcatcacgg catataacga cgccacatct
    15361 ggtagatttc gctgctcaac tgcgtgccca ttttcataat gggtaaaatg aaaattggtc
    15421 agcgttagct tgccaccgtc cggcaaatgg cgacacatca gcaaatgatg gcgaaaatgc
    15481 gactgcggcc agtgcgccga ccagaaattg cccatcacat aatcgctttg ttgctgctcg
    15541 cagagatcaa aacggtacat cgactgccag tgctgatgat gattaaactg caacacccaa
    15601 tcatcaccct cttgcaacaa ccgatactct ccgtgtggcg tggtttgcat gatatccggc
    15661 attaaacgaa tcggcgcggt tagcgtctgc ccaccgaaac cgacatcagc aatccatttt
    15721 tccccttcca gttccaccaa cagcaaacga tgggtgcgcg gcggtaatgc tggcggattt
    15781 gataacacta cgcgccctaa caagctgcga acgttaaacc ccagctcgcg taacacccgc
    15841 tcaaacacgc cattctgctc aaaacagtaa ccaccacgac gggctatcac cagtttctct
    15901 tccagcgatt gatcatcaag ctgtatttcc ctcggcagca aaacgtcgag gttttcaaac
    15961 ggaatggtgc aattgtgttt caggtgcaat gcacgaagcg tatcaatatt ggccgcagca
    16021 gctcccgacc agttaatacg ggcaaaataa tgattcagaa tgggcgtcat caggcgtttc
    16081 ccttagtggt tttctcaact tataacctgg aatagacgcc gcaccggcta ttttgtgcga
    16141 tcgcggacat gcatcacgtt cgcaaatcgc caatcccgtt aatttcgtta cttattgcgt
    16201 tacgagctac gcttttaaaa cacccttcat agcgaggcga tatcgtgagc caattcattc
    16261 ccgtcgaatt acaccatgcc agccgtctgt taaatcatgg cccaactgtc ttgatcacca
    16321 gtttcgatga gcaatcccag cggcgtaaca ttatggctgc agcctggtca atgccggtgg
    16381 agtttgaacc tccacgcgtg gcgattgtgg tagataagtc gacatggacc agggagttga
    16441 ttgagcgtaa cggtaaattt ggcatcgtta tcccaggcgt tgcagcgact aactggacgt
    16501 gggcagtggg aagtgtatct gggcgtgaag aggataaatt ttattgctat ggcattccgg
    16561 tggtgaaagg tccggtgctt ggtttgccac ttgttgaaga gaaatgtctg gcgtggatgg
    16621 agtgccgatt gctaccagcg acttccgcgc aagagaaata cgacacgctg tttggcgaag
    16681 tggtatcagc agcggcagac gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg
    16741 ataagctcaa tacgttgcat catttaggcg ctggcacgtt tgttaccagc ggcaagcgag
    16801 tcaccgcggg ctaaagaatc atatgcagca accaagttgc ttaagcaatg tttcaatatg
    16861 agttaaaagc atattttatc tcacgattgc atatttttag tgaagattaa tttatcctca
    16921 ccaacatcat agtaattgcg tattatagct tcattgataa agccctgtta tgtataaaat
    16981 gcgcgggcgg cactaaaatc gacatagctg gaggtctcta ctaaaagtag tcttgcctgt
    17041 ttaattgtga gctccttttc tatttcgtta atcagcagat gtcccactcc ctggcgctga
    17101 tgggaaggac tgatccagtg cattaataaa ttccagacat catttgtcat cacttcagga
    17161 gcgcaatagg caatccctgc cagtcctaag tgttctgcac taaaccataa atcggcagat
    17221 tgacctgaga aataatgtgt tagtgttttt tgtacatgat gaaggccatc ttgattaaat
    17281 tgtccactgg caagcagcat atccagtaat ggttcaacat ctgaaggttt gactggtcgt
    17341 ataatcatgg aattccttat ctattgatga atgtttgaaa attgtttgcg tggaacatgg
    17401 ttaatctgag ccaatatttt tgagaaagaa tagatgggta tttatataa
//
LOCUS       DAZUCC010000098        17390 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZUCC010000098 DAZUCC010000000
VERSION     DAZUCC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.098"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..1436
                     /gene="tnaA"
                     /locus_tag="ACHY09_002779"
     CDS             21..1436
                     /gene="tnaA"
                     /locus_tag="ACHY09_002779"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFS3885733.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1528..2775
                     /gene="tnaB"
                     /locus_tag="ACHY09_002780"
     CDS             1528..2775
                     /gene="tnaB"
                     /locus_tag="ACHY09_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFS3885734.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2907..4082
                     /gene="mdtL"
                     /locus_tag="ACHY09_002781"
     CDS             2907..4082
                     /gene="mdtL"
                     /locus_tag="ACHY09_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFS3885735.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4057..5016
                     /gene="yidZ"
                     /locus_tag="ACHY09_002782"
     CDS             4057..5016
                     /gene="yidZ"
                     /locus_tag="ACHY09_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFS3885736.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5173..5922
                     /locus_tag="ACHY09_002783"
     CDS             5173..5922
                     /locus_tag="ACHY09_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFS3885737.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5944..6510
                     /gene="yieF"
                     /locus_tag="ACHY09_002784"
                     /gene_synonym="chrR"
     CDS             5944..6510
                     /gene="yieF"
                     /locus_tag="ACHY09_002784"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFS3885738.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6564..7901)
                     /gene="adeP"
                     /locus_tag="ACHY09_002785"
     CDS             complement(6564..7901)
                     /gene="adeP"
                     /locus_tag="ACHY09_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFS3885739.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8068..8733
                     /gene="yieH"
                     /locus_tag="ACHY09_002786"
     CDS             8068..8733
                     /gene="yieH"
                     /locus_tag="ACHY09_002786"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFS3885740.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8800..9273
                     /locus_tag="ACHY09_002787"
     CDS             8800..9273
                     /locus_tag="ACHY09_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFS3885741.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITHLPGLDISCPETDSLS"
     gene            complement(9355..10077)
                     /locus_tag="ACHY09_002788"
     CDS             complement(9355..10077)
                     /locus_tag="ACHY09_002788"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS3885742.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLHNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLIVIADKAAAAELALG"
     gene            complement(10092..11261)
                     /locus_tag="ACHY09_002789"
     CDS             complement(10092..11261)
                     /locus_tag="ACHY09_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFS3885743.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPALKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11288..12904)
                     /gene="bglH"
                     /locus_tag="ACHY09_002790"
     CDS             complement(11288..12904)
                     /gene="bglH"
                     /locus_tag="ACHY09_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFS3885744.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(12990..14384)
                     /gene="bglB"
                     /locus_tag="ACHY09_002791"
     CDS             complement(12990..14384)
                     /gene="bglB"
                     /locus_tag="ACHY09_002791"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFS3885745.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(14403..16277)
                     /gene="bglF"
                     /locus_tag="ACHY09_002792"
     CDS             complement(14403..16277)
                     /gene="bglF"
                     /locus_tag="ACHY09_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFS3885746.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     MPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(16411..17247)
                     /gene="bglG"
                     /locus_tag="ACHY09_002793"
     CDS             complement(16411..17247)
                     /gene="bglG"
                     /locus_tag="ACHY09_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFS3885747.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIVKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4419 a         4388 c         4066 g         4517 t
ORIGIN      
        1 aataaatgaa ggattatgta atggaaaact ttaaacatct ccctgaaccg ttccgcattc
       61 gtgttattga gccagtaaaa cgtaccactc gcgcttatcg tgaagaggca attattaaat
      121 ccggtatgaa cccgttcctg ctggatagcg aagatgtgtt tatcgattta ctgaccgaca
      181 gcggcaccgg ggcggtgacg cagagtatgc aggccgcgat gatgcgcggc gacgaagcct
      241 acagcggcag ccgcagctac tatgcgttag ccgagtcagt aaaaaatatc tttggttatc
      301 aatatactat tccgactcac cagggccgtg gtgcagaaca aatctatatt ccggtactga
      361 ttaaaaaacg cgagcaggaa aaaggcctgg atcgcagcaa aatggtggcg ttctctaact
      421 atttctttga taccacgcag ggccatagcc agattaacgg ctgtaccgtg cgtaacgtct
      481 atatcaaaga agccttcgat actggcgtgc gttacgactt taaaggtaac tttgaccttg
      541 agggattaga acgcggcatt gaagaagttg gcccgaataa cgtgccatat atcgttgcga
      601 ccatcaccag taactctgca ggtggtcagc cggtttcact ggcaaactta aaagcgatgt
      661 acagcatcgc gaagaaatac gatattccgg tggtcatgga ctccgcacgc tttgctgaaa
      721 atgcctattt catcaagcag cgtgaagcag aatacaagga ctggaccatc gagcagatca
      781 cccgcgaaac ctacaaatat gccgatatgc tggcgatgtc cgccaagaaa gatgcgatgg
      841 tgccgatggg cggcctgctg tgcatgaaag acgacagctt ctttgatgtg tacaccgaat
      901 gcagaaccct ttgcgtggtg caggaaggct tcccgacata tggcggcctg gaaggcggcg
      961 ctatggagcg tctggcggta ggtctgtatg acggcatgaa tctggactgg ctggcttatc
     1021 gtatcgcgca ggtgcagtat ctggtcgatg gtctggaaga gattggcgtt gtctgtcagc
     1081 aggcgggcgg tcacgcggca ttcgttgatg caggtaaact gttgccgcat atcccggcag
     1141 atcagttccc ggcacaggcg ctggcctgcg agctgtataa agtcgccggt atccgtgcgg
     1201 tagaaattgg ctctttcctg ttaggccgcg atccgaaaac cggtaaacaa ctgccatgcc
     1261 cggctgaact gctgcgttta accattccgc gcgcaacata tactcaaaca catatggact
     1321 tcattattga agcctttaaa catgtgaaag agaacgcggc gaatattaaa gggttaacct
     1381 ttacctacga accgaaagta ttgcgtcact tcaccgcaaa actgaaagaa gtttaattaa
     1441 aacactcaga gtggctataa ggatgttagc cactctatta ccctacatcc tcaataacaa
     1501 aaatagcctt cctctaaagg tggcatcatg actgatcaat cagaaaaaaa acactctgca
     1561 ttttggggtg ttatggttat agccggtaca gtaattggcg gtgggatgtt tgctttacct
     1621 gttgaccttg ccggagcctg gtttttttgg ggcgctttta ttcttattat cgcctggttt
     1681 tcaatgttac attcagggct attattatta gaagcaaact taaattatcc cgtcggatcc
     1741 agttttaaca ccatcaccaa agatttaatc ggtaacacct ggaacattat cagcggtatt
     1801 accgttgcct tcgttctcta catcctcact tatgcctata tctctgctaa tggtgcgatc
     1861 attagtgaaa cgatatcaat gaatttgggt tatcatgcta atccacgtat tgtcgggatc
     1921 tgcactgcca ttttcgttgc cagcgtattg tggataagtt cgttagccgc cagtcgtatt
     1981 acctcattgt tccttgggct gaagattatc tcctttgtaa ttgtttttgg ttctttcttc
     2041 ttccaggtcg attactccat tctgcgcgat gccaccagca ccacggcggg aacatcttat
     2101 ttcccgtata tctttatggc tttgccggtg tgtctggcgt catttggttt ccacggcaat
     2161 attcccagcc tgattatttg ctatggcaaa cgcaaagata agctaatcaa aagcgtggtc
     2221 ttcggttcgc tgctggcgct ggtgatttat ctcttctggc tctattgcac gatggggaat
     2281 atcccgcgag aaagctttaa ggcgataatc tcctcaggcg gcaacgttga ttcgctggtg
     2341 aaatcgttcc tcggcaccaa acagcatggc attatcgagt tttgcctgct ggtgttctcc
     2401 aacttagctg ttgccagttc gttctttggt gtcacgctgg gattgttcga ttatctggcg
     2461 gatctgttta agattgataa ctcccacgcc gggcgtttca aaaccgtgct gttaaccttc
     2521 ctgccacctg cgttgttgta tctgatcttc ccgaacggct tcatttacgg gatcggcggt
     2581 gccggactgt gtgccaccat ctgggcggtc attatccccg cggtgctggc aatcaaagct
     2641 cgcaagaagt ttcccaatca gatgttcacg gtctggggcg gcaatcttat tccggcgatt
     2701 gtcattctct ttggtataac cgtaatttta tgctggttcg gtaacgtctt taacgtgtta
     2761 cctaaatttg gctaaatcct tcaagaagcc agccattcgc tggcttcttg cctctcagga
     2821 aatcacttat gtccaaatgg caactcgcct gatcctcctt caccacgtat gctttgcgtc
     2881 accttactat caggacgctt tagcccatgt cccgcttttt gatttgtagt tttgccctgg
     2941 ttttacttta tcccgcaggg attgatatgt acctcgttgg tttaccgcgc atcgccgccg
     3001 atctcaatgc cagcgaagcg cagttgcata ttgcgttctc cgtttatctg gcggggatgg
     3061 cggctgcgat gttatttgcc ggtaaagtgg ccgatcgttc agggagaaag cctgtcgcca
     3121 tacccggcgc ggcgctattt attatcgcct cggtgttctg ttcactggct gaaaccagcg
     3181 cgttatttct tgcagggcga tttctacagg gactgggcgc gggctgttgt tacgtagtgg
     3241 cgttcgccat tttgcgcgac acgctggatg atcgacgtcg ggctaaagtg ctgtcgttac
     3301 tcaacggcat tacctgcatc attccggtgt tagcgccggt gctcggacac ctgattatgc
     3361 ttaaattccc ttggcagagt ctgttctgga cgatggcaac gatgggcatc gcggtactga
     3421 tgttgtcttt gtttatttta aaagaaacgc gccctgcggc ccccacaact tcggacaaac
     3481 cacgagaaaa tagcgagtca ctgcttaatc ggtttttcct cagccgtgtt gttatcacca
     3541 ccctcagcgt ttcggtgatc ctcaccttcg tcaacacgtc gccagtattg ctgatggaaa
     3601 tcatgggttt tgagcgcgga gaatacgcca ccattatggc gttgaccgct ggcgtcagca
     3661 tgaccgtttc attctccacg ccgtttgcgc tgggaatttt taagccacgt acgttgatga
     3721 tcacctcaca ggtgttattc ctggcggcgg ggatcaccct tgccgtttca ccttcccatg
     3781 cggtttcgct gtttggtatc acgctgattt gcgccggttt ctcggtaggt tttggtgtgg
     3841 cgatgagtca ggcgttaggg ccgttttcat tacgcgcggg cgtagccagc tcgaccttag
     3901 gtattgcgca agtttgcggt tcgtcactgt ggatttggct ggcggcggtg gttggtatcg
     3961 gcgcatggaa tatgctgatc gggattctga ttgcctgtag catagtgagc ctgttgctga
     4021 ttatgttcgt cgcgcctgga cgcccggttg ccgctcatga agaaatccat caccacgctt
     4081 gatctcaatc tgctgctctg tctgcaactg ctgatgcagg agcgcagcgt gaccaaagcg
     4141 gcgaagcgga tgaacgtgac cccttcggcg gtgagtaagt cgctggcaaa gttaagagcg
     4201 tggtttgacg acccgctctt tgtgaactca ccgctggggc tgtcgcccac accgctaatg
     4261 gtcagcatgg agcaaaatct ggcggagtgg atgcaaatga gcaacctgct gctggataaa
     4321 ccgcaccacc agacaccgcg cggcctgaag tttgagctgg cagcagaatc accgctaatg
     4381 atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac gttacccgca ggcgaccatc
     4441 aaattacgta actgggatta cgattcctta gatgccatta ctcgtggtga agtggatatt
     4501 ggtttttccg gtcgcgaaag tcatccccgt tcgcgggagc tgttaagctc gctaccgtta
     4561 gccattgatt atgaagtgct gtttagtgat gtgccctgcg tctggttacg caaagatcat
     4621 ccggcactgc atgaagcatg gaatctggac acctttttac gttatccgca tatcagcatt
     4681 tgctgggaac agagcgatac ctgggcgctg gacaatgtgt tacaggagct ggggcgcgaa
     4741 cgcacgattg ctatgagcct gccggaattc gagcagtcac tgtttatggc agcacagccc
     4801 gacaatctgc tactggcgac cgcaccgcgc tactgtcagt actacaatca actccaccaa
     4861 ctgccgttgg ttgctcttcc tctcccgttt gacgaaagcc agcaaaaaaa gctggaagtc
     4921 ccttttaccc tgctgtggca taaacggaac agccataatc cgaagatcgt ctggttacgg
     4981 gaaaccatta aaaatcttta cgcgtcgatg gcataaccga atcgtatgaa acgcgaccca
     5041 atttcacaat aaaatgtaaa aaagttgtaa taagcttgtc tgaatcgaac ttttagccgc
     5101 tttagtctgt ccatcattcc agtaaatgat tactcttgta ttcataatgg accattaagc
     5161 atggagcgaa aaatggcgac tcactttgcc cgagggattt taacggaagg acatctgatt
     5221 tctgttcgtc tcccctccca gtgtcatcaa gaagcccgaa acattccccc tcatcgtcaa
     5281 agccgttttc tggcgtccag aggtttactc gcagaactga tgttcatgct gtatggcatt
     5341 ggcgaattgc cggaaatcgt caccctgccg aaaggaaaac cggttttcag cgataaaaat
     5401 ttgccttcgt tttctatttc ctatgccggg aatatggtcg gcgtggcgtt aacaaccgaa
     5461 ggtgaatgtg gcctcgatat ggaactacag cgtgcgacgc gcgggtttca tagcccacac
     5521 gcgcccgata accacacctt ttccagcaat gaatcgctat ggatcagtaa acaaaacgat
     5581 cctaacgaag cgcgggcgca gctcatcacg ctgcgccgaa gcgtgctaaa actaaccggt
     5641 gatgttttga atgacgatcc gcgcgatctg caactactgc ccattgccgg acgcctgaaa
     5701 tgtgctcatg taaatcatgt agaagcgtta tgcgacgcgg aggacgtgct ggtgtggtcc
     5761 gtggcggtca cgcccacgat tgaaaagctc agtgtctggg agttagatgg caaacatggc
     5821 tggaaaagcc tgcccgatat tcacagccgc gccaacaatc ctaccagccg gatgatgcgt
     5881 tttgcccagc tctctaccgt gaaggctttt tcgccaaatt gatagacaac cacaggagtc
     5941 atcatgtctg aaaaattgca ggtggttacg ttactgggga gcctgcgcaa aggctcattt
     6001 aatggcatgg ttgcacgtac cctgccgaaa attgctccgg cgagcatgga aatcaatgcg
     6061 ttaccttcca ttgccgacat tcccctgtat gacgctgacg tacagcagga agacggtttt
     6121 ccagcaacag ttgaagctct ggcggaacag atccgtcagg ctgacggtgt ggtgatcgtc
     6181 acgccggaat ataactactc ggtaccgggt gggctgaaaa atgccatcga ctggctttcc
     6241 cgcctgccgg atcaaccgct ggctggtaaa ccggtattga ttcagaccag ctcaatgggc
     6301 gtgattggcg gcgcgcgctg tcagtatcac ctgcgccaga ttctggtttt cctcgatgca
     6361 atggtgatga acaagccgga atttatgggc ggcgtgattc agaacaaagt tgatccgcaa
     6421 accggggaag tgattgatca gggtacgctg gaccacctga ccgggcaatt gaccgcattt
     6481 ggtgagttta ttcagcgagt taagatctaa ataaaaaacc cgccagcaat catgcatggc
     6541 gggtttttaa cgcgctatcg attttagtga gcgtcgataa agacaatctt caggataaac
     6601 agcagcgcaa cgatgattac gcacgggcta aggtcacgca gacgtccggt accaatcttc
     6661 atcacgcagt aggagataaa gcccagcgcg ataccttcag taatcgagaa gctgaacggc
     6721 atcatcacag cggtaataaa cgccggaaca gattcagtaa gatcctgcca gttcacgcga
     6781 gcaagacttg aggtcatcaa cacgccaacg taaatcagcg caccagctgc agcgtagcct
     6841 ggcaccatcc ccgccagcgg cgacaggaag ataaccagca ggaacagcag accaacaacc
     6901 actgccgtca gaccggtacg accgccaacg gaaacgccgg aagaagattc aatatacgcc
     6961 gtaacagaag aagtaccgat aaacgaaccg gtcaccgaag agatactgtc gacatacagc
     7021 gcctgcttca tgcgcgggaa tttacctttc tcatcagcca gacctgcttt atcggtcacg
     7081 ccaatcagcg taccggagga gtcaaacaag ttgaccagca tgaaagagaa aatcacccct
     7141 gccagcccaa ggttaaacga cccggctaaa tctacatgac ccacgacact cattacgctc
     7201 ggcggcgcag aaacgatgcc attatagtgg acatcaccca gcatccagcc cagcagcgtt
     7261 gtcaccacga tggaaaccag caccgccgcg tgaatattgc gcgaggccag aatggcgatg
     7321 atgaagaagc cgaggatccc cagcagtacg ctgtgagaag tcagattacc gatgctcacc
     7381 agcgtttccg ggttagcgac aatcacacct gcattcttca gtcccatcat gccaataaac
     7441 aggccgatac cgctggtaat gcccacacgc agactcaccg gaatgttggc tatcatccag
     7501 tagcgaacgc ggaaaatcgt cagtaacagc agaccaatcg cgccccagaa gattgcgccc
     7561 atcccgacct gccacggcaa gcccatcgcc tgcacaacca caaaagcgaa gaacgcattc
     7621 aggcccatag cgggtgccag tgcaactggc aggttagcaa acagtcccat cataatactg
     7681 ccgaatgcag caatcagaca ggtagtaacg aagacggcgc tggtatccat gccagcaacg
     7741 ccaagaattt gcgggttaac aaaaacgatg taaaccatcg tcaggaaggt ggtaaaaccg
     7801 gcgatcactt cggtccgtgc cgtcgtgcca tgttcgcgca gtttaaacac gcgttccagc
     7861 atcccctggc cagaagcctg ggttgtgtgt tgttgactca ttatctattt ccgaacaagg
     7921 agggaaaatc cgtcgctatc gtataccaaa atgagacaat atggacggct gaagataact
     7981 ttttttcatt gtatttgctt atacggcaac gattgcgtga tgcgaattgg catgacgaaa
     8041 acgttaaact aacgataaag gaaagacatg tcccagatag aagcggtatt tttcgactgc
     8101 gacggtacgc tggtcgatag cgaggtcatt tgctctcgcg cctatgtcac gatgttcagg
     8161 gaatttggca ttcacgttga cctggaagag atttttaccc gttttaaagg cgtaaaactc
     8221 tacgaaatca tcgacatcat cagtaaagaa cagggcgtga cgatggtcca ggccgatgcc
     8281 gagcacgttt accgcgcaga agtagcacgt ctgtttgata ccgaacttga ggacatcgca
     8341 ggagcgaacg aattgctggc gagcattaac gtgccgatgt gcgtcgtctc taacggtccg
     8401 gtcagcaaaa tgcagcattc gcttggtaag ctcaacatgc tgcactactt cccggaaaaa
     8461 ctgttcagcg gctacgatat ccagcgttgg aagcccgacc cggcgttaat gttccatgcg
     8521 gcaaaagcga tgaatgtgaa cgttgaaaac tgcattctgg tcgatgactc cagcgccggt
     8581 gcgcaatcag gaatcgatgc tggtatggaa gtgttttact tctgcgctga cccgcacaat
     8641 aagccgatcg ttcatccgaa agtcaccacc tttacccatc tttcgcaatt accggaactg
     8701 tggaaggcgc gtggttggga tattacggca tagttattca cactcccttc acttactcca
     8761 cttaaattgg cacgcaaagg taagtaaagg gagtttgata tgtctgtttc acgtcgggta
     8821 attcatcacg gactgtattt tgcggtttta gggccgttaa ttggtgttct gtttcttgtc
     8881 ctctacatat tcttcgcaaa agaaccgctg attcttttgg taataataca ggtacttcca
     8941 ttgtttctct taatgtcgat aactacggga gctattcctg caatgttaac tggcgtaatg
     9001 gtcgcctgtc tgccggaaaa gatcgggtca cagaaacgtt atcgttgtct ggttggcggc
     9061 ataggtggag tcgttatcac cgagatctat tgcgctgtta tcgtacatat taaggacatg
     9121 gcttcctcag cgttgtttga aaacattctt tctggtgaaa atctcgttgt ccgcatcatc
     9181 ccagcattgc tggcaggcgt agtgatgagc agaatcatta cacatctacc cggattggat
     9241 atttcatgtc ctgaaacaga ctctttaagc taagcggaat gctttatcta tttgtcatta
     9301 aatgttggag aacaggccgg agcgtaattc acacatccgg ccttattttt aagcttaacc
     9361 gagcgctaat tctgcggcag cggctttatc agcgatgact atcagcgaag ggtgcaactg
     9421 caatacggaa gcaggaacat cttcggtaac cggaccttgc agaacatttt tcagtgcctg
     9481 cgctttaccc gcgccactga caattatcag caagtttttc gccgccatga tgcttttcgg
     9541 ccccatcgtc acgtagctgt caggcactaa tgaaaagtcg ccacccaact cgccatgcgc
     9601 cacaatatca accatctctc cctggatcgg gaactccacg gtttgctcat ggaaatgggt
     9661 cgtatttggc agattgccac aaaaatgacc atctacacct aatcccaaca ccaccaaatc
     9721 caggccgcct tcacgcgcca gtttctgatc atgctcgcgg tagttatcaa tagtgagctt
     9781 ctggatattc tcttctttga tccctgcagg ggtgaaaaac agattatgca gattggtaat
     9841 cgttacgcct tccccttctt taccgcgaaa tggaatttca tcgaaattgt agaaatagca
     9901 gttatcgtac cagggcttac ctttaaccag agtgatgaga tattcgtaca tgcctttggg
     9961 cgtgctcccg gcggtaattg ccaggttaac gcgacgcatc tttgacatat aaccaagcag
    10021 atgatgtgcc gcgacacggc tcatttcctg gtaatcttcg gtaatgatca atttcatctt
    10081 aatgccttat attatttgaa taatttctgt acaaaggctg cataggctgg tcgccagaca
    10141 tccatttcat ggttcagacc gggatattcc tggtaatcaa acttaatttt tttctgctca
    10201 agctcagttt tcagcccggc gatatccttg ccggttacga catctttatc gcccacgacc
    10261 acagtaaaat tacgtagttg ctggttaata gctgccggct cgttcagccg ggcagcgacc
    10321 ccttcatctg gtacggtcgt cgtggtaaca ccactgaatg tggccagcca gccaaagctt
    10381 tccagatgat tcattcccga taccagtgcc tgatacccac cttgtgaaag ccctgccagt
    10441 gcgcgaccat cggcatcttt acggacatta aaacgcttgc taatcagcgg gataatatcg
    10501 ttcatcaatt cgcgatctgc cgctttagca tttaacggat aaaagacttt acgtctctcc
    10561 tgaggaacga aatcttcggg aataatgccc ttcgcatcgg tttcggtatc cgggatcacc
    10621 accagcatcg gtttaatttt cccttcagcc agcaggttat ccatgatttg cgggatacgc
    10681 ccctgatcaa tagcggaacg tccggtatca ccaaagccgt gatagaaata gagcactggc
    10741 aaaggctcgc ctatgccggt gtatcctggc ggggtccaga catacatctg acgttcggat
    10801 tgcaatgcgt tggagtggta agttatggcg atcaaatcac catgcacaac agaacgtgta
    10861 tccagataac tgcccggtac cagaatcata ctggagttaa cctggcgctg aggcttagtc
    10921 attgctgtac ctgtatcaat gctgcgtaca ccatcaacat tgaaaaaata ctcgtacagg
    10981 ttgcctttca gagcaggcgt gcgccacgac cagactcccg cttcgtcttt ggtcatcggg
    11041 tgaatattgt ccggaaccgg aacacccacc actacagaga cattttttgc cccaggggca
    11101 aagtagcgaa aggtcacgct gttatcggta ttgacctgag tcacatactg tttcaccgga
    11161 atagtgggtg ccgggctggc tggcatatcc gctgctatgg cccattgagc agaaataccg
    11221 ctggcaatag ccagcgttaa agcagcaatt tttatattca tcgtaatatt ccttttatta
    11281 agttccatta ccaccagatt tcagcctggg caccgacagc aaactggtcg tctttattgt
    11341 cttcaaaggt gaagccatcc agttcgtttt ccagagcttt gatatatgta gcgtagaaac
    11401 gaatttccgg gcgcgaggtc aacatactgg tattgacttt aaaggtatgg aagagcgtgg
    11461 ttttataacc agactcatta tatttattgc tattcgcatc tttgttttgc tgggtgaaat
    11521 agcccagttc aacacctgtc tggttatatt ggtcccaaat ataggccggg cgaacaaccg
    11581 cacgaataga ttcgaaatca gaatgtgcgc ctgtttcgta gctataaata tcgttaccga
    11641 aggagtaaac aatcgcgtta gccacaatga aatgatcgcc gatataagct tcgccctgcg
    11701 aagtcagacg aactgcagtt ccgcctgtgt gatcaccata ataacgacca ttaaaggtag
    11761 taaatggact tgcaccagca taacgaccaa agttactggc gatagagtta ttcgcaacca
    11821 ggaaggagaa ttcgttgaag ccacccttat caaatttctg cgttaaagat gtaccaaaca
    11881 tccaggtatc tttccagtca taatacccgt tattatcctg attatctttt tccgatgcgc
    11941 tttcatttgc tgagacataa cgaccgctca ccattaaggt ggctttatcc cataacggga
    12001 tatctttata gcgtaaatca atggtattgg tattaatctg ctgtttgttt tgcaggctgc
    12061 gatcgtaatc atcgacatct tcacgaacca gcgcgatatc aattttaccc ggaccgactt
    12121 tccagttttc cagacctaca cctgcggcag catcggtacg ctgcgttttc cagtcaagca
    12181 tctggatttc aattttcggc gcaccgtgtt tacccaccca gaaatcagcc tctggcgcaa
    12241 agggcaggaa acctttggtg gtaacgtaca tatcagagaa ctgcataaag ttctcaccac
    12301 cggcgttatc gccaaaccag ccagtagagt actgctgacc aacgttacca tccatcatca
    12361 cgatggcatc aacccgtttg ccgttttcgt tgtagacacg ttgttttaac tgcaaatcaa
    12421 accagccgga gtattcgtta ccaaagcggc ccagagaacc aattgcccat gatttaggtg
    12481 aaccatgaga ggctgttccc caaccagaac ggtagtagcc gttgtaacta aagccgattt
    12541 catctttcac aaatttactg aaatctttca gagtcatgga agaataaatc gagcctgtct
    12601 cactggcatt tttctcttca tttttaacca gtacagcatc aggctttgcg gcacgactgg
    12661 tcgggaatgg attggcggca tacccttgat cattcgtact tacgctacga ttcaccgtcg
    12721 ctggcgtata ctttttcttc tcttcatctt tatatttttt cagttcgctt tgcgtttctc
    12781 ttaacgcctt ttctaatagc tcaaggcgtt gttccactgt taatgattca gccaatgatt
    12841 gtgcggaaaa ggctaacggt gccattaata agatggcaga ggtaataaga tttcgtctaa
    12901 acataatatc cctttatggt gcaaagaaag aattaacgca tcgcatccag actgttctga
    12961 atgcgacgat aattaaggtg ctttaatggt tattttttta atgacagccc ccgagttttg
    13021 atcacttctg cataccatcc gaagcttttc ttgcgcgttc ttgtgaggct tccttcacca
    13081 ttatcatcac gatccacata aataaagccg tagcgcttag acatttgtga atgagatgca
    13141 ctgactaaat caattggccc ccaactggtg taccccataa tatccacacc atcggcaatc
    13201 gcttcattta cctgtaccag gtgatcgttt aaataggcaa ttcgataatc gtcctgtatc
    13261 gaaccatcca cttcaacgct gtcttttgcg cctaatccgt tctcgacaat aaataacggt
    13321 ttttgataac gatcccaaag cgtatttaac agaatccgta atccaaccgg atcaatttgc
    13381 cacccccact ctgaactttt cagatgcgga tttgggatca tattcagtat gttgccctgc
    13441 gcatttttat taatgctttc gtcgtgggaa acacaaccgg tcatgtaata actaaaagag
    13501 atgaaatcta cggtatgttt taaatcttct gcgtcacttt cagtcatctc aatggtgata
    13561 ttgtggtcgc ggaagaaacg ctgcatatag ccgggatact gaccacgcgc ctgaacatca
    13621 ccaaagaaca tccagcgccg gttctcttcc atggcctgca acatatcctg tggctggcag
    13681 gtgagggggt aaaccagccc accgagaagc atattgccaa ttttcgcttc ggggatcagg
    13741 ctatgacagg ctttaactgc ccgcgcactg gcaaccagtt gatgatggat cgcctgataa
    13801 acttccgcct cgccactctc ttctgccagc cccactcccg tgaatggcgc gtgtaatgac
    13861 atgttgattt cattaaatgt cagccataac acaactttat gttggtagcg ggtgaagacc
    13921 gtgcgggcgt aatgctcgaa gtgatcgatg accgcccgat tagcccaacc gccgtagttt
    13981 ttcaccagcc catatggcat ttcgtaatgg gataacgtta ccagcggctt gatccccgcc
    14041 tgcgccattt catcaaacag ccgatcgtaa aacgctaacc ccgcttcatt cggttcggct
    14101 tcgtcgccct gagggaaaat tcgcgcccag gcaatggaaa tacgcagaca ggtgaagccc
    14161 atctcggcaa ataacgcgat atcttccggg taacggtgat aaaaatcgat ggcgacatct
    14221 ttgatattct ctttccccag gatgcgcggt tccatttttc ccattacgcc atgaggctgt
    14281 aaatctgagg tcgtgatccc tttgccatct tcctgccagg cgccttccac ctgattggca
    14341 gctgttgcac caccccaaag aaatgtttct ggaaatgctt tcataattaa ctccttttat
    14401 cgttagcgaa tgatggataa cagcggctca ccagcgttta tctgcgccgt gccgtggggt
    14461 aatacgtccg taaaatcatc gctattactg attaataccg gcgtcgtcag atcaaatccg
    14521 gcctcgcgaa tagcagggat atcaaaagaa atcagccgat cgcctgtatt gaccttgtca
    14581 cccacgttga cgtgagcgga aaagaatttg ccgtccagtt ttacggtatc gataccgaca
    14641 tgaatcagga tctccacacc atcatctgac tcaatgccaa tggcgtgtaa tgtggcgaac
    14701 aacgaagcaa ttcgacctgc caccggagaa cgcacttcac caaccgaggg cataatggca
    14761 atacctttac ccaacaggcc gctggcaaac gtggtatcag cgacgtgaat gagcggcaca
    14821 atctctcccg tcatcggtga acagataccg ccctgctcag gtgtaataac ctctggtgtt
    14881 ttctcttgct gggcaacttg cgctgactga ctcttagcgg tgatgaaatg aaacatcacc
    14941 gtaccgacaa atgcgcaacc gatggcaatg acaccgccaa taacgctggc ccatacggtg
    15001 aaatcaatcc ccgttgacgg gatggtttgc atgaaggtga aaatacctgg caaaccaaag
    15061 gagtaaactt tcgtttgcgc gtagccaatg atggtggccc ccaatgcccc acttatgcag
    15121 gcgataacga aggggtactt acgcggcaga ttgacgccat ataccgctgg ttcggtgata
    15181 ccaaacaaac ctgtcaacgc cgctgatccc gccaccactt ttttctgtgc atcgcgttcg
    15241 cagaggaaga cgccgagcgc cgccccgact tgcgccataa tggcgggcat taacagcggg
    15301 atcatggtgt cgtagcccag cacggtgaag ttattgataa ttaaaggtat caaaccccaa
    15361 tgcagaccga acatcacaaa gatttgccag aagccgccca ttaccgcgcc tgcaaatgca
    15421 ggaaccgcct gataaagcca gagataaccg gcagcaatca gttcacttat ccaggttgat
    15481 agcggcccca ccagcagaaa tgtgatgggc gtgatgacca tcagacatag caagggtgtg
    15541 aagaaatttt tgattgccga aggtaaccac gcattaagtc ggcgttccag aatgctgcac
    15601 aaccaggcag aaaaaataat gggaataacc gatgacgagt aattcaacaa tgtgaccgga
    15661 atacccagga aatccaggcc cagcgcatcc actttttgcc cgttctcgaa ggcagtcaga
    15721 attaatggat gcactaacgc tccgccaatc accatggcag taaatggatt gccgccgaag
    15781 cgtttccctg cggtgtagcc caggattatc gggaagaacc aaaacaaggc atcactggcg
    15841 ctaaataaaa ttaaataagt accactttgt tcggtcgtcc actgaaaagt gagtgccaga
    15901 gccaacatac ctttcaggat cccggttgcc gccatcaaac cgatcagagg cgtaaaaata
    15961 cctgaaataa cataaacaaa gcggtttaac agattacctt tatcatcatt ttccggtgcc
    16021 tgttgcgctt tttcgccaag gcctgccacg ctgttaaccg ccaggaagac atcggccaca
    16081 tggttaccta tgacaacctg aaactggcca ccgctttcca ccaccataat aataccgggg
    16141 gtctttttca gtacctcagc ttgcgctttg ctttcatcct ttaacttaaa acgtaatcgc
    16201 gttgcacaat gcatcagact cacaatgtta tctgcgcccc cgactcctgc gactattgtt
    16261 ctggctaact ccgtcataac ttgccctcta ccgctttgcg gcaaaactcc aaaaaaaaac
    16321 ctgaaaaaaa cggcctgacg tgaatcaagc aatttttttc aggttttgcc cgcttagtgc
    16381 ggtaacaatc ctttattcag taataatatt tcagtgttct ttgcgcacgc gctctatatt
    16441 tatggctaaa aacataatct ctgcaggtga gattttacgt tgatactgca aaccaataaa
    16501 aatggcgatc cgctccgcac attgccatgc ttgcgggtaa ttttgcttta ctgcttgttg
    16561 taatgattca tcactatcgt taatcgatgc atgttcaata atacgccagg ataaaaattt
    16621 cagatgcgta accagtcgct gataactcaa gctttcttcc tggtaattaa ggctgaactg
    16681 aaattttatt aattgcagca tttctcgcat taattgcgtg acacctgcaa catcctccat
    16741 atttccgctc atttgggcac tgaccagatg catggcaata aagcccactt catctttcgg
    16801 taactgcacg cccaaccgtt taacaataat ggttaacgct tcttccccta gctggaactc
    16861 tttcgggtaa agtcgctgga tatcccacag cagcgggttg ggcagtagca cgttttgctg
    16921 aaagcgttta atcgcaaact ggcaatggtc agttagcgag atataaatac tgtcctgcaa
    16981 ctttcccagg cgctcctgcg ccagagagat aatacgatca caggttgcca tcacctcaag
    17041 aggcatatga cttaagagtt cgcttaatcg cccgttcagt tcatgactgc tcaaggcata
    17101 ctctttttct attccacttg agttaattct ttcgcctggg cgtttttgaa agccaattcc
    17161 gcgccccatg acgacttttt cccgctgttg atcatcaata accaccacaa cattattgtt
    17221 gagaattttg gtgatttgca tgttcatagc aaggaccttt tttataaaca aaaaaacccg
    17281 acttcaccag tattctctgg ttatgtcagg ttttgcctgc gaatgcagta acaatccagt
    17341 cattaattaa tggtttttat aacgaacatc caggttcgaa aattaattta
//
LOCUS       DAZUCC010000099        17330 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZUCC010000099 DAZUCC010000000
VERSION     DAZUCC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.099"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..532
                     /locus_tag="ACHY09_002794"
     CDS             14..532
                     /locus_tag="ACHY09_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFS3885748.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            548..1087
                     /locus_tag="ACHY09_002795"
     CDS             548..1087
                     /locus_tag="ACHY09_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFS3885749.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1180..2757)
                     /gene="guaA"
                     /locus_tag="ACHY09_002796"
     CDS             complement(1180..2757)
                     /gene="guaA"
                     /locus_tag="ACHY09_002796"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS3885750.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2826..4292)
                     /gene="guaB"
                     /locus_tag="ACHY09_002797"
     CDS             complement(2826..4292)
                     /gene="guaB"
                     /locus_tag="ACHY09_002797"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS3885751.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4454..5830
                     /gene="xseA"
                     /locus_tag="ACHY09_002798"
     CDS             4454..5830
                     /gene="xseA"
                     /locus_tag="ACHY09_002798"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS3885752.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDNAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQLKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANLRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(5899..13950)
                     /locus_tag="ACHY09_002799"
     CDS             complement(5899..13950)
                     /locus_tag="ACHY09_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFS3885753.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPAKTHEVAFTAKPSEFSADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPTVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLIAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKTTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQTSETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEDGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNYYWSSLAGMSGQHYAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGVNR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEASLTLTQPGGVGVKTHITAK
                     MRSDYTASDEKDVIFTVITSPDSDKARMWGHMLGIIEANNIFKRPRLADETDNELGAV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANVIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDHDVSTSSRAQAKVG
                     ETITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQPQGVGLATPLNIAPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVSSPDGTLTENNEVWSRVSQANTSSTSKG
                     GCGANMLPRRSQLNALYDANSGNAVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDMPFTLKRGDGYTRSNEKHIAGGGDALVAPVVINSGLADETSLNDTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTAALYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDSTNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQPTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMPVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGDVV
                     AMDDLIVQPLSGSAVTLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14071..15048)
                     /locus_tag="ACHY09_002800"
     CDS             complement(14071..15048)
                     /locus_tag="ACHY09_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFS3885754.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEESAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADHVTLTSDLKGARPTGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDDDRGRYIGIEITPTT
                     QTGTPNVGTALHLYDISTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLAIPATNDEARTNLNGPARDGKEALTIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15103..17280)
                     /gene="sinH"
                     /locus_tag="ACHY09_002801"
     CDS             complement(15103..17280)
                     /gene="sinH"
                     /locus_tag="ACHY09_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFS3885755.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYIINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDEWLLGGNIFYDYDFTRGHRRLGLGGEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKVVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGADYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPRLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDSNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4077 a         4609 c         4304 g         4340 t
ORIGIN      
        1 ataacaagga atagtgatga aatttaaaaa atgtcttctg cctgtggcaa tgttagcgtc
       61 attcactctg gcaggatgcc agtcaaatgc tgatgatcat gccgccgatg tttatcaaac
      121 cgatcaactg aataccaaac aagaaactaa aaccgttaat attatttcca ttcttcccgc
      181 aaaagttgcc gtagacaacg cccaaaataa acggaacgca caagccttcg gcgcgcttat
      241 tggcgctgtc gctggcggtg ttatcggcca caacgtcggg tctggcagca attccggaac
      301 gacggcaggt gcagttggcg gcggagctgt aggcgcggca gcgggttcta tggtgaatga
      361 taaaacctta gtggaaggtg tttctttaac atataaggaa ggcaccaaag tgtatacctc
      421 cacccaggtg ggtaaagagt gccagtttac gacaggttta gccgttgtta ttaccacgac
      481 gtataacgaa acacgtattc agccaaatac caaatgtcct gaaaagagct aataatcagg
      541 aggagtcatg aagaaagttt ttctttgcgc catcttagcc tccttaagct atccggctat
      601 cgcctcatca ttgcaggatc aactctcggc tgtcgcagaa gcggaacagc aaggtaaaaa
      661 tgaagagcaa aggcagcatg acgaatgggt cgcggagcgc aacagggaaa tccagcaaga
      721 gaagcaacgt cgcgcaaacg cccaggccgc ggctaataaa agagcggcaa cggcagcggc
      781 gaataagaaa gctcgtcagg ataaactgga cgccgaagcc actgcggaca aaaaacgcga
      841 tcaaagttat gaagatgagc tacgcagctt agagattcag aaacaaaaac tggcgctggc
      901 gaaagaagaa gcccgcgtta agcgagaaaa cgaatttatc gatcaggaac tgaagcacaa
      961 agctgcgcaa accgatgtgg tgcaatctga agctgacgca aacaggaata tgactgaagg
     1021 cggtcgcgat ctgatgaaaa gcgtgggcaa agcagaagag aataaatcag atagctggtt
     1081 taactaaacg atgttagtaa cttcaaacct ataattcttt aggataaaaa accctctgta
     1141 gtaacagagg gttttgttca ttcatagtgc agggtcaaat cattcccact caatagttgc
     1201 tggtggcttg ccgctgatgt catacaccac gcgggaaata ccattcactt cattgataat
     1261 gcggttggaa acgcgaccga ggaaatcgta cggcagatgt gcccagtgtg cggtcataaa
     1321 gtcgatggtt tcgacagcac gcagagagac aacccagtca tacttacgac catcgcccat
     1381 tacgccaacg gaacgtactg gcaggaagac ggtgaatgcc tggctgactt tgtcgtacag
     1441 gtccgcttta cgcagttctt caatgaagat ggcgtcagca cggcgcagca ggtcacaata
     1501 ctctttcttc acttctccca gaacacgaac gccaaggcct ggtcccggga acgggtgacg
     1561 gtacaacatg tcatacggta ggcccagctc cagaccaatc ttacgcactt cgtctttgaa
     1621 cagctctttc agcggttcaa ccaagcccat cttcatctct ttcggcaggc cacccacgtt
     1681 gtggtgagat ttgatgacgt gtgctttacc ggttgcagaa gcagcagatt cgataacgtc
     1741 agggtagata gtgccctgcg ccagccactt cacgtcttcc agtttcagcg cttcttcatc
     1801 gaatacttca acgaaaacgc gcccgatgat tttacgtttt gcttccggat cattttcgcc
     1861 agccagcgct gacaagaagc gatcttctgc cggtacgtga acaatattca gaccaaagtg
     1921 atcgccaaac atatccagaa cctgctctgc ttcgttgaga cgcagcaggc cgttgtcgac
     1981 gaatacgcaa gtcaggtttt taccgatagc gcggtgcaac agcattgcgg ttacggagga
     2041 atccacacca ccagagaggc cgaggatgac tttatcgtcg cctacctgct cgcggatgcg
     2101 agctacagca tcgtcgataa ttttcgctgg cgtccacagg gcttcacact ggcagatatc
     2161 acgcacaaaa cgctccagca tgcgcatacc ctggcgggtg tgagtcactt ccgggtggaa
     2221 ctgtacgcca tagaagcgtt tttcttcgtt agccataatg gcaaacgggc agctttcggt
     2281 gctggctacg gtgacgaagt cggacgggat agcggtaact ttatcgccgt ggctcatcca
     2341 gacatcgagc agcggtttac cgtctgcggt cagagcatct tcaataccgc gaaccagtgc
     2401 gctgtcgttt acgacttcaa cctgcgcgta gccaaattca cgttcgttag aggcttcaac
     2461 gtgaccgccc aactgcattg ccatggtctg catgccatag caaacgccga ataccggtac
     2521 gcctgcttca aaaacatact gcggcgcacg cgggctgttt tcttcggtgg tgctttccgg
     2581 gccgccggaa agaataatgc cgcttgggtt gaagtcacga atttgtgctt ctgtcacatc
     2641 ccacgcccac agttcgcagt aaacacccag ttcacgcacg cggcgcgcaa ccagttgagt
     2701 gtactgggaa ccgaagtcca gaatgaggat gcgatgctta tgaatgtttt ccgtcattga
     2761 cgcttattcc gaggcaagtg aaacagataa tataaatcgc ccgacatgaa gtcgggcgaa
     2821 gagaatcagg agcccagacg gtagttcggg gactctttag taatggtcac gtcgtgaacg
     2881 tggctttcct gaatgcccgc accgctgata cgtacaaact ccgctttagt acgcagttcg
     2941 tcgatagtac cacagccggt cagccccata caggagcgca ggccgcccat ctgctggtga
     3001 atgatctctt tcaggcgacc tttataggcc acgcgacctt cgataccttc cggcaccagt
     3061 ttgtcggcag cgttgtcgct ctggaagtaa cggtcagaag aacctttgga catcgcgccc
     3121 agggaaccca taccacggta agatttgtaa gaacggccct ggtagagttc gatttcaccc
     3181 ggagattctt cagtacctgc cagcatggaa cccaccatca ccgcgcttgc gcctgcggcg
     3241 atagctttgg cgatatcgcc tgagaaacgg ataccgccgt cagcgataac cggaatgcca
     3301 gtaccttcca gggcttctac tgcatcagca acggcggtga tctgcggaac accgacacca
     3361 gtcacgatac gcgtcgtaca gatagaacca gggccgatac ccactttaac ggcactgcaa
     3421 ccggcttctg ccagcgcgcg ggcacctgca gctgttgcca cgttgccgcc gatgatttgc
     3481 aggtcaggat atttagcgcg agtttcacgg atacgctgca gaacaccttc agagtgaccg
     3541 tgagaggagt cgatcagcag aacgtcaacg cctgcggcaa ccagcgcatc aacacgctct
     3601 tcgttaccag cgccagcacc aaccgctgca ccaacacgca gacggccttg ctcgtcttta
     3661 caggcgttcg gtttacgttc tgctttctgg aaatctttca cggtgatcat gccgatcagg
     3721 tggaattcgt catcaaccac cagcgctttt tcaacgcgtt tttcgtgcat ttttgccagc
     3781 accacttcac gggcttcgcc ttcacgcacg gttaccagac gctcttttgg cgtcatgtaa
     3841 acgctaaccg gttgattcag atcggtcaca aaacgcacgt cacgaccggt gatgatgccg
     3901 accagttcgt tttcttcggt aacgaccggg tagcccgcaa agccgttacg ctcggtcagt
     3961 tctttcactt cgcgcagcgt cgtggttggc agaacagtct gcgggtcagt caccacacca
     4021 gattcgtgtt ttttcacacg gcgaacttct tctgcctggc gttcaatgga catgtttttg
     4081 tggataaagc cgataccgcc ttcctgagcc agagcaatag ccaggcgcgc ttccgttacg
     4141 gtatccattg ctgcggaaag catagggata ttcagacgaa tagttttcgt cagctgggtg
     4201 ctgaggtcag cagtattcgg cagaacggta gaatgtgcag gaacgaggag aacgtcgtca
     4261 aacgtcagag cttctttagc gatacgtagc atgggcaata tctcgaccag agtggttaat
     4321 aaatattgcc gcggcattat acagagcgta accgattgca tctacccctt tttgcaaaaa
     4381 atgcttgcta tccccggagg gcgggttact atcgactgaa taacctgctg atttagaatt
     4441 tgatctcgct cacatgttac cttctcaatc ccctgcaatt tttaccgtta gccgcctgaa
     4501 tcaaacggtt cgtctgctgc ttgagcatga gatggggcag gtgtggatca gcggcgaaat
     4561 ctctaatttc acacaaccgg cttccggtca ctggtacttt acgcttaaag acgataacgc
     4621 ccaggtgcgc tgcgcgatgt tccgcaacag caatcgccgg gtcaccttcc gcccgcagca
     4681 tgggcaacag gttttagttc gcgccaacat tacgctgtat gaaccgcgcg gcgattatca
     4741 aattattgtc gaaagtatgc agcccgccgg tgaaggttta ctgcaacaga agtacgaaca
     4801 gcttaaagcg aagttgcagg ctgaaggttt gttcgatcag caacttaaaa aaccactccc
     4861 ctcccctgcg cactgcgttg gggtgattac ctcaaaaaca ggtgccgcac tgcacgatat
     4921 tttgcatgtg ttgaaacgcc gcgatccgtc tctgccagtg ataatctatc ccactgccgt
     4981 tcagggcgat gacgcaccag ggcaaatcgt tcgcgccatt gagctggcga atcttcgcaa
     5041 cgagtgcgac gtattaatcg tcgggcgtgg cggtggctcg ctggaagatt tatggagttt
     5101 taatgatgag cgtgtggcgc gggcgatttt tgccagtcgc attccgatag tcagcgccgt
     5161 cgggcatgaa accgatgtga caattgctga ctttgttgct gatctacgtg cgccaacgcc
     5221 gtcagccgcc gctgaagtgg tgagccgaaa tcagcaagaa ttgctacgtc aggtgcaatc
     5281 gacccatcaa cggctggaaa tggcgatgga ttattatctc gccaaccgca cgcgtcgttt
     5341 tacgcagatt catcatcgct tgcagcaaca gcatccgcag ctccggctgg cacgtcagca
     5401 aactatgctc gaacgtctgc aaaagcggat gagctttgcg ctggaaaatc agcttaagcg
     5461 tgccgggcaa cagcagcagc gattaacaca gaggctaaac cagcaaaatc cgcagccgcg
     5521 tattcaccgc gcacagacgc gaattcaaca actggaatat cgtttagcag aaaccctgcg
     5581 cgcacagctt agcgccacgc gtgaacgttt cggtaatgca gtaacacacc tcgaagccgt
     5641 aagcccactg tcaacgctgg cgcgcggtta tagcgtcacc agcgccgctg atggcgcggt
     5701 gttaaaacag gttaagcaag tgaaagtggg tgagacactg accacacgac tgggagatgg
     5761 tgtggtgatt agtgaagtca gcgccgtaac aaaaacccgc aaatcacgta aaaaaacgtc
     5821 taatccgtag accggataag aggcgccagc atcgcatccg gcaataactc ccaggccacg
     5881 ccagatccgc catgctggtt actggaagca ggtcaccaga cccggtttat ttattagcgt
     5941 gatactacaa tcctgtgctc cggtaccttc gtccatccca cattgttgag aggacaaata
     6001 aggaaaactg ggtgaagacg gccatcccag tgaattaaaa acagtatgca acaactctaa
     6061 ttcattgtat gtcggtgcat tttctatgct gccacactgc cttgcgatat cccatttact
     6121 ggcatcaatg atatgtgcag aagcccagtt ttcgtagtta ataatcggtg aggatacggg
     6181 attagccccg gacggcaatt ctgtcgttaa caacggtcga tgtagcgatt taccattcac
     6241 cactaccgtg tctgccatat gtccccaaaa gtgcgccgag gaaacatttg ggctggttgg
     6301 taccgtaaaa atagcgtcca gcgtgtctgt cgcggaagcg taattagcca gagtaaccgt
     6361 cagtggcgtt ttataaccag gggtattatc ctggcggagc gtaaaacttg ccgtcccatc
     6421 cgcgcctgtc atgccactaa tggtattacc gctttcactc agcgtaacag ccgaaccaga
     6481 taacggctga acgatgagat cgtccatcgc caccacatca ccatacaacg ttgccccagc
     6541 gttacgcggc gatgcctcac cgcgtttcaa agtaaattcc acattcggca ccgtattccc
     6601 ggcgctatct ttcaccgtca cggtaacggg catggcttcg ccttttttca ctttcgccgc
     6661 ctgagtggct gcatcaaacg ctgttgaggt aagtgtgacg ctggaaaccg ttgaacgcgc
     6721 gttagccagg caaggctgaa atgattttgt tgaacccgta tacagcgtgc catttctcaa
     6781 atcaaccgac tggtctttac catcggcgga tttatcctct gcccaccaag tgtaatacgc
     6841 aggccagcca gtttcagtga caaacgtgtt attcggataa cgcgcagaca acgaacttaa
     6901 atcattgaat agtggctgcc ttgctacttc acaagggctc acactcgcat ctgatttctg
     6961 tgttggggta aagtacggcc agtcttcatt attgttattg atcttgccga cgccagaagt
     7021 cgatgtcagt tcactccaca atttcgggcg ttcaaatacc gcaccattca ctgccgtcaa
     7081 ggttgctggc atatgccccc agtagttagc cttcgcagaa tccgggctgg tcactaccgt
     7141 gaatatcagg tcctgattag ccgtaacctg cggatttcgc gccaatgaag ctgtaaacgg
     7201 cgttaccaga ccgacggtgt tattttgggt aacgttgaac gttatcgtgc catcactgcc
     7261 ggtgaagccg ctccatgccg acgtattctg gttgaaggag atacgcgccg atgtcggggt
     7321 gacttcatct aaaatgagat catctacggt attggaggat acagagatcc ctgaacggct
     7381 tttcgcctgc acgcgcttga gcgtaacacc ttcgccgcca acaggattac cattggcatc
     7441 tttgatcgtc accacgatgg gaagcatctc accttttttc acagatgacg tgggggtacc
     7501 accattgctg gcagttcggc cctcatcata ggcagtcgat gacagcgtaa ggcttgctgg
     7561 tgccgaatgt ggctgcgtca gacagagcat taaagcgtat ccattaatag tcgtcgtcga
     7621 ttgtcccgtt ttcaaattaa tgacgccgta ataccacgtc aacgcgtcag tggtgctcat
     7681 ttcataagtc caccaatttc cacttccggc agttataggc aaccccaaat ctgtcagtat
     7741 tgtcccgcct ggatgatcat tatatagccc ctgtaactca ttgagacgcg gctgatactc
     7801 aaccgggcaa tccggcgtaa ctttattggt ataaaaaagg tatgaccacg actcattatt
     7861 attacttacc gaggacccca aggtcgcttc tgctgccagt agcggtcgtt taaaggtcac
     7921 ccccgcccta ctggtaaacg tgtcgggcat atgaccccag tattttgctt tcggtgagtt
     7981 cggactggtt atcacagtga aaataacatc catcgtggca ctttttgtgg tgtcgcgcat
     8041 caatgttgca gtgagcggag ttgccagccc ataagtctcg ttttgcgtca catcaaaagt
     8101 cgctttacca tcacttcctg tcgtcagaag tgcctgtgcc ccattattat agagtgacat
     8161 gtttactccg gcaggggtga ccggcacgat agtaagatca tcatacgtag aatctagcct
     8221 gttttttgca cgatccaacg cagtcccgcg tatgagatta aagtttgcgc ctggcaacag
     8281 atttcctgcg ctatcccgca caatcaccgt catccggatc gccgtacctt tcgccgccgt
     8341 tgcactaggg cgaccatcgt tgctggccgt tttctccgca tccagtgccg tggaggtcat
     8401 ctctatagaa gctgccactg catgagggtt taccagacag gacacaaatg ccacggtgtt
     8461 tgataatgcc ctggagcttg atccattcga taaattaata gactgatcgt accagacgtt
     8521 cgcgtaggca attttatcgt aagcccacca ggtgttagtc agcggaaggc ctaaatcggt
     8581 ccctatcgca ccattaggat gttcactgta taactcttga aattcgctta ataacggttg
     8641 ataaggttcg ccgcagtctg atttgctgac atcatttttt gctgccgtat tcaaagaaag
     8701 ccatgattca ttattagcca tggttgactg ccccgctgga tgctcggcag taagcaatgg
     8761 ccgcttaaag gcaattcccg accgggtagt aaacgtttca ggcatatgcc cccagtaact
     8821 tgccagtggc gtatccgggc tggttatgac ggtaaacatg accggtaatt tgttggattc
     8881 tatcgtcggc tgatacaact gggcgtacat attggttacc aggcccatag aatcatcttg
     8941 ttttaccgta aaggaggtag tcccatccgc gccagtcacc ccataaagca ggcttgaaga
     9001 actattcatc ctcgcagaag cgcctgccgc gttaattacc gtaatcgact tatttatcat
     9061 ctcggcattt tgacggttct tcgagccttc acgtctgaga ataaagggga cattgggcac
     9121 gctatttccc tgcgcatttt tagtcaccac gcgaatcacc gcgtcttcgc cttttttcac
     9181 tttcaacgca ctcaaggtgt cgtcaacttt gacaaattgc gccgtatcat tcgcctcaac
     9241 gatgatctga ctggcaacgg cgacaggcgt ggtccggcag ctcagtaatg caccaccaga
     9301 tgagggcatc tgcgtttcat ctcctgtctt cagcgaaacg acatcatacg taaaggtttg
     9361 agtgaacgta tctgcaacgg tattcgagat atagaacctc gacactggcc agccaatagc
     9421 atcatgtaat gcgctactcc cctggtgcgc ataaatggcc ctaagttcat tgtcagttgg
     9481 aacatactcc gcgccacaac catttatcga ggtatcgtta gcctggcccg acgggaacgt
     9541 agaccaggcc tccccctctt ccaccagggt accgatactg gtggtagcac tattctcgtc
     9601 cctcagtaaa ggccttcgat aaataagacc gtcatcgcct gtcgccgttt cagccatatg
     9661 cccccagaat ttggcttttg gcgaatccgg gctggtaagc accgtaaaga caacgggaag
     9721 cgtcgattta gtgctgctgg aggaatccaa cgctgccgtg agttctgttt ttagcccggt
     9781 agtctggtct tgcttcaatg tgaaagtggt gatgccatca gcccccgtca cgccataaat
     9841 aaccagggtc gttgaaagaa aatcatcttg agtatttccc caggcatccg ttaccgccag
     9901 cgaagcaatc gacgtcgtag ttgacacatt ggccctgttc acgctggtgt ttcgtttgag
     9961 ggtaaatgcg gtatttccca cagggttacc ctgcgcatct ttagtggtca ctcgtaatac
    10021 ggcttcttcg ccttttttcg ctttgacaac attattcgta ctgtcaaatt ttgtggggtc
    10081 tgcagcttca agaacaattt ggcttgcctt aacaatcggc gtcgtctggc aggtaagcag
    10141 ttcttttgtg gcagatggaa ttatcgaact ggtgcctgaa ctgagattga cggccttgaa
    10201 gtcacgctgt tccaggctac tactacctgt cgtgctggaa aggtaacttg aggcgacggg
    10261 ccagccctga accgttttca tcgcattgcc gcggttggct tcatacaatg actccagccc
    10321 ggcctgggag ggaacatatt cactaccaca accattagag gatgtgctta tagcctgggt
    10381 ttgggtaaat tgcgcccagt cttcattctc ttctgcaatc gctgttcgac ccgacgtact
    10441 ggataactct ttcaacaata atgggcgttt aaagaccgtt ccatctgcag ccgtcactgt
    10501 ttcaggcata tgcccccaca ttttcgcctt atcgctgtcc gggctggtga caacggtaaa
    10561 aatggtatcg agggtcgctt ttttcgttgt gtctgaatag agcgcggcgg tcagcgacgt
    10621 ttttgtgcca tgggtatccg ggcgggtaat agtgagaatt ttagtgccat cgctgccggt
    10681 catcgcgctg tatgctgcgg cggtatcatt gagcgaagtt tcatccgcca ggccactatt
    10741 gatcacaacc ggcgcaacca gcgcatcacc gccaccggca atgtgttttt cgtttgatcg
    10801 ggtataacca tcgccacgct tcagcgtaaa aggcatatca cccaatggat tgccctgagc
    10861 atcttttacc gtcaccttca cctgtagcgt ttcgcctttt ttcagcttcg ccgcatttgc
    10921 tgccgcgttc cactgcgccg ggtccaccac ttccagcgta atgctgctgg caggattgtt
    10981 agccgtcgtc aggcaactga cataaaccgc cgtgctaccg ccaacggtcc gcgcgccatc
    11041 gttgagggct atggtgtagt aatgcggcac ctgatccgcg ggcgaactgc tccagtaggg
    11101 ctgacgctgc gttggccaac cgtgtaccgt ttgaacggcg ttaccgctgt tagcatcata
    11161 gagcgcgtta agctgggaac ggcgtgggag catattggca ccacagccac ctttactggt
    11221 gctggaagtg ttggcctggc taacgcgcga ccacacttcg ttattttccg ttaatgtgcc
    11281 gtccgggctg gacacttccg cagccagccg gggtcgggca aaggttaatg catcgacggt
    11341 aatcgtctca tccatatgac cccacatctg cgcgccaacc gcatccggac tggtgagcgt
    11401 agtgaaaatc acattgtaat taacagtatt gggaatatag gagtttacag gcgcaatatt
    11461 cagcggcgtc gctaagccca caccttgcgg ttgcttaatt tccacagtag caaaaccctg
    11521 cgcatccgta gtaccggcat agactaaaga cggttgcgat gttccgtaga gtgtgccatt
    11581 catctcgatt gcgccgcggg ttggatcatc aaaacctgta gtctgcccct gacgattttt
    11641 ccccatatct ttagtgatgg tgaacgcggt atacggcacg ggcgcattat tcaaggcatt
    11701 aaaggtgcgt accgtcatgg taatcgtttc acccacttta gcctgcgctc tgctgcttgt
    11761 ggaaacatca tgatcggtgg tcgcagcaat ctgggcgacc atctcattgc ccgaacaaga
    11821 aaggtagttt tgcttaaagc cggaataggt gtcgatgttg cccgtggcca ggttcaccga
    11881 cgcgtgcgac gtctcttcca ccgcactaag atacgctttt tgcaacgttg gccagccata
    11941 ttcggtgccg atgacattgg ctggatgtgc ggcaaacaaa ctgtgcaatg agctttggct
    12001 cggaatatga cctaaaccac actcagcctg catactgcta ttctgatcaa acagcgccca
    12061 gtcttcgtta ttttcccgca ctgctcccag ttcgttgtcg gtttcatccg ccagccgtgg
    12121 gcgtttaaag atattattcg cttcaataat gcctaacata tgtccccaca tccgcgcttt
    12181 gtcggaatca gggctggtga tcaccgtaaa aataacgtct ttctcatccg aggcggtata
    12241 atcactgcgc attttcgcgg tgatgtgtgt tttcaccccc acgccgcctg gctgggtcag
    12301 cgtcagagag gcctctccgt tagcatcagt gatcccttca taggtatgcg cgtcaacttt
    12361 gcgaacgtta ctgccaccat caatttgtac cgggtgcgtc tcccacgcag cgtcagtttg
    12421 attcttacgg ttaacaccgt catccagatg cagcgaaaag tagtagtaag ccagcggctg
    12481 atcgttgttc ttgctatcgg taatcgtcag gcgtaaagaa gcctcttccc ccacctttac
    12541 tttcatcgct tttatcgtcg aatcataact gcccggcgtc agtaccagct tcggttcaac
    12601 atcgggcgct tctttatcga cacagctcac aataaacgtc gtatcgtccg gtttttgcgc
    12661 ttcgctgcgg ttcgaaacat ccgccgcata gtgctgcccg ctcatccccg ccagtgatga
    12721 ccagtagtag ttaccctgca tcggccaacc agccacatcc tgcactgacg ttggcaccac
    12781 cgtcgccagc gcaccgaact gacgcattcc gggcaaaatg tcgcaatgtt tatccgcacc
    12841 actccaggta aaggtggacc aggtttcatt gtgatcgtta accgtaccgt cttcattatc
    12901 gacttccgcc gccagttttg gtcgactaaa agtgtaatca cgggccttca gcgtatcggg
    12961 catatgaccc cacattgtgg cctgcgggac atccgggctg gtgaggacgg taaagatcac
    13021 tgccgtttca ctggtctgag caatacccgc gaggctcgcg accaacggcg tttttacacc
    13081 agggccgttg gcctgggtga ccgtaaccgt ggcaacacca ttcgcatcgg tagtaccacg
    13141 atattccgtc gcggtggtgg ttaattcggt cgtgcccagt tttaccggcg cagtattgtt
    13201 cactacaccc tggcggtttt cagcatcttt gcgtttgata acaaaaggaa tattccccac
    13261 aacattaccg acgcaatctt tggtggtgac cgttaacgtg atactgccgc caaccgtcgt
    13321 tttcgccgca ccggaggatt cgtcatagcc cgcggcgctg acggaaataa ggtcaccctg
    13381 cgcgggcgca atcttcagcg tcactttctg atcggcaagg gtcagaacac tttcataggg
    13441 atagcccgtc gaggtaagta gatataacag cggcatggtc tgctgggggt ctggctgcgc
    13501 ccaggcgatc agcgaacgcc cggcaaggcc ctgtgcgcaa aatgagcgat ctgcgactgg
    13561 ctgtgggtcc agtaaggtgt ccggcgtcgc ggcatccgcc caaactaaag atactgtcgg
    13621 tggcgtttgg ttttcccaac gtggtgtggc aatgatatcg ccttctctgt cttgcgggtt
    13681 ggtgacgagc aatttagaga cgctaacatc cgcgctgaat tcactcggtt tagcggtaaa
    13741 cgctacctca tgcgtctttg caggatccag aaagacactg ccctggtaga cgggtatgct
    13801 ggcactgtcg gcggtgggca cggtaccgtt aaattcgccg accgtatctg aagagtcttg
    13861 ccatgcagat tctgcccatg accacccagg cagcattgcg cttatcagca atgcgcataa
    13921 cacaaggatt ttattcaggg tcagggacat gacagaaact ccaccggttg ttaaacggac
    13981 gcttgtcgca tcaacatatt ttgcttaatc cgggattaag caaaatatgc ggaaacagca
    14041 ttcccttgcg ggaatgctgc tgagtacgcg ttatttgtgt ttgtaaataa tgtgaagttt
    14101 gtaaccctgt accccgtcgc cgtttgtcgg aatggtaaga gcctctttac catcgcgcgc
    14161 aggaccgtta aggttggttc tggcttcgtc gttggtcgca gggatggcca gattgttatt
    14221 atcgaagctt gagttaacga taccacctga agtgcccagc tgctggctgc tgccagaaag
    14281 tacccaacgg aagtcataat tggcggtaac gtcagcatcg gtacccgcat caaacttgcc
    14341 gtttttgttc tcatccgagt acagtttggc cacgtaggtt ttgccaagtt cgattggcgt
    14401 gctgccgttg ataaggttga tactggtacc atcaacaaag atggcgactt tcaggttctg
    14461 gttaaccacc ggccctggcg caacgttatc gctgtcgctt ccgccgccgc tggcagtgga
    14521 aatgtcataa agatgcagcg cagtcccgac gtttggcgta ccggtctgcg tggtcggcgt
    14581 aatttcaata ccgatataac gaccacggtc gtcatcggta agagtgtaac tggtcacctt
    14641 cgttgccagc tctttcggat cgcccccctg agcgtccttg tagctcatcc agcggatagt
    14701 atcgatagtc tttgcgttat cgcccaatcc cccaagatcc acgtcgcctt cggtatcacc
    14761 aatttcccaa cttacggtta atttatcacc cgagtaaaga cgcttgtccg tttcactgcc
    14821 tgtcgggcga gcgcctttta gatcagaagt caacgttaca tggtcggcat ctgtcgtagc
    14881 gcttccccct tccgaataga gccacggaat ggttccctgg aaaatattgg tttcgccgct
    14941 gtcgacccag gcgctttctt ccgccagcgc cacaggtgcc acacaataac ctgccagtgc
    15001 cagcgccaat gcgatccggg tcagaccgcg tcttttatcc tgcttcattg atgctctcct
    15061 taatgaacct tcagcgactg gccgactggc cttacgccaa tcttattttt tggtatacag
    15121 cacgcgtaaa ccgtatccct gcaagccatc tcccgcttgt gcgccatagg tttgcgctgc
    15181 ttcgcggtta gtggccggaa tcacaatgtc ctcattggaa gtgttcgcct gtgcacccac
    15241 ttcaccattg atgctcttgc cggtaaattc ccacttgtag tcgtactgcg ccatctcttc
    15301 gtcagtcagt ttttcgacct gctggaagac accgtcttta ttgctgtcac gccacaaaac
    15361 aaatcgataa gtgttgttga gcggcagcgg ttctttatct ttacctatca ggttgctggg
    15421 tttagccgcc cccacacgat agataaatgc atttagccca ccaatttctg ccccgaggaa
    15481 agtgacatcc acatagttac tgtcgtcgta gggcgcagct tttgctttcc agcgataggt
    15541 gccgggtaag gtactggaga gggtgacagt acccttttct ttatcttgtt tagcgataaa
    15601 aacgcagggg ttgtcggtcg agcaaggctt gttggttaac gtcactcgtt tgtcctgcgc
    15661 gtcgtaaagc cgcggcgtga ggttatcttc cggcagcgca tcgccgttgc ggtcggtgtt
    15721 atcgccaaag ctgtcggtta catcgagatc catcaccaca ccatcaacgc catctgcctg
    15781 ggcgctggct tcgtggctgt ggttgttctc ttccggcacg ttcgcgatgt tattaacaat
    15841 caactcaatt ttacggtcct gctgaacggt gatttccacc acaccagagt tctgtcggtt
    15901 gcccttttca tcttccagcg ccagacgtac tcgccaggta ttgagttttt cttgttcggc
    15961 tttgtcagag ctgtactgcc aggcgggcag caccaggttc cagtggttat tgttaccctc
    16021 tgtcagggta ttctctttga taaccggcat aaccagcgtg cgggcagcat tgtttttcgc
    16081 ctgccatgac gcagcgacaa ttttgtagtg gtggttgata agcgcgttaa tggtccactt
    16141 acatttttcc agcccgaccg ccgcttcagg cgtgtcttta atgacacact cggggtgttc
    16201 gttggtggca tccgctttta accacagggt gacatcaagc ggatcttttt ctttgtattc
    16261 cagaacaata aagttattac gctcaacaaa atcatgacga ctgcccatca acgagtgagc
    16321 cgctttcact ttatcgctat ccaattgatc ttttagcgga acgccgaact gataattaag
    16381 agtggcatta atgctgacat cgctgttatc tccggtcccc gctttatagt ccgccccaac
    16441 ggttagtaat ggcaccggtt gataattcag tccaagtgtt accgcgtagg gatctttctc
    16501 caaattatcc gtaccaaaaa gggcaacttc atcgccgtaa tattgctcga agacaacttt
    16561 gccccccagt tgcggataag aaggtaacca ggcttcggcg cgaatatccc agccgcgcgc
    16621 aggacgctcc tcataaaaat cgaaatcttc ggagtctttc cagtcagaaa gcggatgata
    16681 atagttgcca gagaatttta aataatcagt ccacgcttcg ccgcctaaac ctaaacggcg
    16741 atgtcctcgg gtaaaatcat aatcataaaa tatatttccg cccaataacc attcatcaaa
    16801 attatatctt accccaagac caatattccc gattgtacga tcttcttttc gctgcgcaga
    16861 aaattgactg aaataaacag tggtttgatt atcgtaccag ggaacaaaat aatcgatgga
    16921 actgccatcc agatcgccgc cctggccaat ggagaggttc gaacggacct taccgtaggg
    16981 agagagagtg tcttcaatat aagattgtgc ggttgaggtg atttgacttt gcagatagct
    17041 tctggcctga gattccagcg cctcaggggt caagttttca aacccttgcg tggcggagtt
    17101 gatgatgtaa tcggctccgc gctctttcag cgatttaccc ttgttggttt gttcatcctg
    17161 ctgtgctgcc tggctaccta aatcaggtaa cgattcgttt tgcttaacat ttatctctgg
    17221 cgccgcgaaa gcagcaccag agataaatgt tagaataata cagcgtttcc atcgcagcat
    17281 gcactttccc tgtgacaatg atacagtcaa aaataagctt taatattgta
//
LOCUS       DAZUCC010000100        17320 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZUCC010000100 DAZUCC010000000
VERSION     DAZUCC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.100"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(32..844)
                     /gene="fkpA"
                     /locus_tag="ACHY09_002802"
     CDS             complement(32..844)
                     /gene="fkpA"
                     /locus_tag="ACHY09_002802"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS3885756.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            1065..1283
                     /gene="slyX"
                     /locus_tag="ACHY09_002803"
     CDS             1065..1283
                     /gene="slyX"
                     /locus_tag="ACHY09_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFS3885757.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(1332..1922)
                     /gene="slyD"
                     /locus_tag="ACHY09_002804"
     CDS             complement(1332..1922)
                     /gene="slyD"
                     /locus_tag="ACHY09_002804"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS3885758.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(2017..2217)
                     /gene="yheV"
                     /locus_tag="ACHY09_002805"
     CDS             complement(2017..2217)
                     /gene="yheV"
                     /locus_tag="ACHY09_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFS3885759.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(2227..4032)
                     /gene="kefB"
                     /locus_tag="ACHY09_002806"
     CDS             complement(2227..4032)
                     /gene="kefB"
                     /locus_tag="ACHY09_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFS3885760.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVTVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(4032..4583)
                     /gene="kefG"
                     /locus_tag="ACHY09_002807"
     CDS             complement(4032..4583)
                     /gene="kefG"
                     /locus_tag="ACHY09_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFS3885761.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            4714..6627
                     /gene="yheS"
                     /locus_tag="ACHY09_002808"
     CDS             4714..6627
                     /gene="yheS"
                     /locus_tag="ACHY09_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3885762.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            6627..7649
                     /gene="yheT"
                     /locus_tag="ACHY09_002809"
     CDS             6627..7649
                     /gene="yheT"
                     /locus_tag="ACHY09_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS3885763.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNNLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            7643..7861
                     /gene="yheU"
                     /locus_tag="ACHY09_002810"
     CDS             7643..7861
                     /gene="yheU"
                     /locus_tag="ACHY09_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFS3885764.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            7915..8784
                     /gene="prkB"
                     /locus_tag="ACHY09_002811"
     CDS             7915..8784
                     /gene="prkB"
                     /locus_tag="ACHY09_002811"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFS3885765.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(8839..9243)
                     /gene="yhfA"
                     /locus_tag="ACHY09_002812"
     CDS             complement(8839..9243)
                     /gene="yhfA"
                     /locus_tag="ACHY09_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFS3885766.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            9545..10177
                     /gene="crp"
                     /locus_tag="ACHY09_002813"
     CDS             9545..10177
                     /gene="crp"
                     /locus_tag="ACHY09_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFS3885767.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            10228..12318
                     /gene="yhfK"
                     /locus_tag="ACHY09_002814"
     CDS             10228..12318
                     /gene="yhfK"
                     /locus_tag="ACHY09_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFS3885768.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12385..13605)
                     /gene="argD"
                     /locus_tag="ACHY09_002815"
                     /gene_synonym="dapC"
     CDS             complement(12385..13605)
                     /gene="argD"
                     /locus_tag="ACHY09_002815"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFS3885769.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
     gene            complement(13691..14254)
                     /gene="pabA"
                     /locus_tag="ACHY09_002816"
     CDS             complement(13691..14254)
                     /gene="pabA"
                     /locus_tag="ACHY09_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFS3885770.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(14286..14888)
                     /gene="fic"
                     /locus_tag="ACHY09_002817"
     CDS             complement(14286..14888)
                     /gene="fic"
                     /locus_tag="ACHY09_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFS3885771.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(14878..15045)
                     /gene="yhfG"
                     /locus_tag="ACHY09_002818"
     CDS             complement(14878..15045)
                     /gene="yhfG"
                     /locus_tag="ACHY09_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFS3885772.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(15150..15722)
                     /gene="ppiA"
                     /locus_tag="ACHY09_002819"
                     /gene_synonym="rotA"
     CDS             complement(15150..15722)
                     /gene="ppiA"
                     /locus_tag="ACHY09_002819"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFS3885773.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            15993..17174
                     /gene="tsgA"
                     /locus_tag="ACHY09_002820"
     CDS             15993..17174
                     /gene="tsgA"
                     /locus_tag="ACHY09_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFS3885774.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILIV
                     LAGLAAILMYVFNTGTPAYMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
BASE COUNT         4204 a         4786 c         4305 g         4025 t
ORIGIN      
        1 cgccgcctgg tcaggcggcg gttcttaatg cttatttttt agcagaatct gcggctttcg
       61 catcagcttc cggctttgca tcagccttcg gcgctggttt cacatccagc agctctacgt
      121 caaacaccag ggtagaattc ggtgggatcc ccggaacacc cgctttgccg taagccagtt
      181 ctggtggaat aaccagtttg atcttaccgc ctttcttgat gttcttcaga ccttctgtcc
      241 aacccggaat aacaccgtcc agacggaaag agagcggttc accacgggtg taagagttgt
      301 cgaactcttt accgtcgatc agcgtacctt tgtagttcac gacaacagta tcgctgtctt
      361 tcggtgcttc gcctttaccg gcttcgacta cctgataaac cagaccagtt gaagaggttt
      421 tcacaccttt ctctttggca aatttctcgc ggtactcttt acctttcgct tcgttatctg
      481 ccgcgtcttt ttccatcttc gcctgagcag aagacttcac gcgagcttcg aatgcttgca
      541 gtgtctgttc gatctcttgg tcggagagtt tgctcttatc agcaaatgca tcctgaacac
      601 cagcgatcag ctgatcttta tccagtttga tgcccagttt ttcttgttct ttaagagaat
      661 tttccatgta acgacccagc gaagcaccca gtgcataagc tgatttctga tcgtcatttt
      721 tgaacgctgc tttgctgtca gcagttgtag caggttttgc agcttcagca gcaaaagtga
      781 tcggtgcatg cagggcaacg gccattgtgg tcgccagcag cgttacttta aacagtgatt
      841 tcatccatat ctccaggatc ggggcatctc accccagggt taactattat cagaagggta
      901 ctataaagcg ttgtcgaaca aatctacata cagacacgcc ctattatcat ctattttcag
      961 actctttttg tttaaattag tttcgatgac cgcgaaatga gtgctgtctc gggcagcaaa
     1021 gttaagtaga atccgcggcg accattcgac aaaagaggtg aatcatgcag gatttatcat
     1081 tggaagcacg cctggcagag ctggagagcc gactggcttt tcaggaaatc accattgaag
     1141 aactgaacgt cacggtgacc gctcatgaaa tggagatggc gaaactgcgc gatcatctgc
     1201 gtctgctgac cgagaagcta aaagccagcc agccgtcgaa catcgcgtcg caggctgaag
     1261 aaacgccacc gccacattat tgaggcgtaa aaaaagcggg gattccccgc ttttttgtca
     1321 ctttttcggt attagtggca accgcaaccg ccgttacctt taccgccgca gcagccttcg
     1381 ccaccgtgtt catgaccgtg atcgtggcca tgaccaccgc agcaaccgtc gtggtcgtga
     1441 tcgtggtgat gatcgtgcgc accgtgaacg tggccatgag ccagttcttc ttcagtcgct
     1501 tcgcgaatcg ccacaacttc aacgttgaat ttcaggttct gaccggccag catgtggtta
     1561 ccatcaacca cgacgtgatc gtcttcaacc gcagtgattt caaccggtac cggaccctgg
     1621 tcggtttcag ccaggaaacg cataccaacc tgcagttcat caacgcccat aaatacgtct
     1681 ttaggaacgc gttgcaccag gttttcgtcg tactgaccgt aagcgtcgtt cgcgccaaca
     1741 gcgacatcaa atttgtcgcc aacttcgtga ccttccagcg ccgtttccag gccagagatc
     1801 agggaaccgt gaccatgcag gtagtccagc ggcgcactca ccggagactc atcaaccaac
     1861 acaccgtctt ctgtacgtac ctgataggcc aggctgacca ccaggtcttt tgctactttc
     1921 atgatatctc ctgagcatgg gaagaatagt ggcgcagatt gtagcggaat tctgcagccg
     1981 tgtactcact agcttaaaaa aactcggcgc atatcgctag tccggatgaa aaatcccgat
     2041 cacttgctca tctttgcgaa cgtgatcgcg ggcttctttg tccgcttctc gcatctgatg
     2101 tccgcactta acacattcaa caatatcaat attattttcg cgccacatcg ccattgaatc
     2161 ctgcgcctga caggccgggc attttgcccc cgcaataaaa cgttttcgga ttgccatctt
     2221 taccctctac tcaaattcat cccagccgtc cagctggcgt cgttcttgtt gcatttcacg
     2281 ctggaaaatc tcttccagtt cgcgtcgggc ttccctggcg cgagaaattt gtacggtgtc
     2341 ggcatgcatt gggatgagct cccgcaacat ccgcatatcc aggcggcgaa aatgcagttg
     2401 cgcccgctgc gcctgatgtg gatgcatgcc tagcgtgacc agcgtcttgc gccccagctc
     2461 taacgcactg gagaatgtct cacgagaaaa ctgcgtcacc cctgcctgta ataactcatg
     2521 cgcctccaca cgtccgcgcg ctcgcgcaag aatatgtaaa tgcggaaagt gctgctggca
     2581 tatttccacc agtttcatgg tgtcttccgg ctcattacag gtaatgacga tagactcagc
     2641 ggcctctgca cccgcagaac gtaaaagatc tacctgcgtg gcgtcgccgt aataaacttt
     2701 gtagccatat ttgcgcatca ggttaacggc gctgatatcc cgctccagca cagtaatgcg
     2761 cattttattt gccatcagca aacgaccaat cacctgacca aaacgcccga agcccacgac
     2821 aatgacctgc ggtttatcat cgttgaccca cggtttttcg tcttcttctt ccggtccgtt
     2881 aaactggcgg gatagccatt tatccaccag cttcatcagc aacggcgtgg tcatcatgga
     2941 aagcgtcacc gtcaccagca acagcgccat ctggtcgccc tgaaacaacc gttgcgaaga
     3001 agcggtagaa aagagcacaa aggcaaactc accgccctga ctcaacacgc cagcaaactg
     3061 catccgctct gagctacgca cgccatataa tcgcgccagc agatacagca cgagaatttt
     3121 caccgtcacc agcacaacca cgcttatcac tacccacaac agatgggtat aaagcacccc
     3181 gagattgagc gacatgccga cagagataaa gaacaaaccg agcagcaagc ctttgaaggg
     3241 atcgatagct gtttccagtt cgtggcgata ttcgctttct gccagcaaca cgcccgcaat
     3301 aaacgtaccg agcgccatcg acagccccag cgcatccata aacaatgcgg aacccaacac
     3361 cagcagcagc gtcgcggcgg tgaacacttc ccgcacgcca gaagctgcaa taaaacggaa
     3421 taccggacgc agtaaatagc gcccgccaat cagcatgccg acaaacgcca gcaccttcat
     3481 gccgatcttc atccagtcga aatgttcgtc tgccgaaccc gccaacaacg gcactaacgc
     3541 cagcgccggg atcactgcca gatcctgaaa cagcagaacc gaaaaaccta gctgcccgga
     3601 ttcgctgcga ttcatccctt tctcacgcat cagttgcaac gccattgcgg ttgaggacat
     3661 cgcaaggcca ataccaccga ccaccgccgc ctgccaggcg aaatccgtca gcatcaataa
     3721 tcccgccagc aacgccgcgc ttaacagcac ctgtgccgca cctacgccaa aaatcgaacg
     3781 ccgcaattgc caaagcttgg agggattcaa ctccaggccg atgataaaca tcaggaacac
     3841 cacgccgagt tccgaaaagt ggaggatctc atcgacgtcg ctaataaaac ccagccccca
     3901 cgggccaatt gcaatccctg ccagcaaata tcccaacaca gcgccaatac ccagccgcga
     3961 tgccagcggc accgcagcca ccgccgcgaa gagaaacaac actcctgcga gtaaaaaatc
     4021 ggaaccttcc atcagcggcc tcctggagac agcggatttg ccagccagtc accgtaggct
     4081 ctggcgtgac tcgccagctc ctgtgcgctt tgccgtctcg cccagtaaat aatgatggga
     4141 ctcaaccaat gcatccggca catgcccgcc gctagttcaa aggggcgcag aacatcgctc
     4201 atcgggtagc gattcaaggc gtcataacgg taagcacttt ccggctcgcc ggtggtaatc
     4261 acgctacgcc agtactttcc cgccagttgg tttcctcccg gcccgctggc aaaaccacgg
     4321 cttaataccc ggtccagcca ctctttcagt agcgccgggc agctataggt ataaagagga
     4381 tgctgaaaga caatcacctc gtgctcgcgc agtaatgcct gctcacgggg gatatcaata
     4441 aaaaaatcgg gatagtgcgc gtaaaggtcg tgcacggtaa cattgctgag ctgcgtggcc
     4501 ggtttaagca gtacccggtt tgccaccgag tcctgagatt ccggatgggc atacagcagc
     4561 aaaactttcg ctggctgaga catcatcccc ctcccggtat ggtttttgtg tatagtcgct
     4621 gttttgggct accattgcgc ccggtgcggc agctcgccca tacattacat tatcataatg
     4681 ataagttaac atagtctgaa catacggcgc cttatgattg ttttttcctc gttacaaatt
     4741 cgtcgcggcg tgcgcgtcct gctggataat gccaccgcca ccatcaaccc cgggcagaaa
     4801 gtcggcctgg tgggtaaaaa cggctgtggt aaatcgaccc tgctggcatt gctgaaaaat
     4861 gaaatcagcg ccgacggcgg cagctacacc tttccgggaa gctggcaact ggcgtgggtg
     4921 aatcaggaaa cgccggcgtt accgcaagcg gcgctggaat atgtcattga cggcgaccgt
     4981 gaatatcgtc aactggaagc gcagctacac gacgccaacg aacgtaacga cgggcacgcc
     5041 atcgccacta ttcatggcaa gctggatgct atcgacgcat ggagtattcg ctcccgtgcc
     5101 gccagcctgc tgcacggcct cggtttcagc aatgaacaac tggagcgccc ggtaagtgat
     5161 ttctccggtg gctggcgtat gcgtcttaac cttgcccagg cgctgatttg ccgttcagac
     5221 ttgctgctgc tcgatgaacc gactaaccac ctcgatctcg atgccgttat ctggctggaa
     5281 aaatggctga agagctatca gggcacgctg atcctgatct ctcacgaccg cgacttcctc
     5341 gatccgatcg ttgataaaat tattcatatc gaacaacaaa gcatgttcga gtacaccggc
     5401 aactacagtt cgtttgaagt acagcgcgcc acccgtctgg cgcagcaaca agcgatgtat
     5461 gaaagccagc aggaacgcgt ggcgcatctg caaagttata tcgaccgttt ccgtgccaaa
     5521 gccaccaaag cgaagcaggc ccagagccgt attaagatgc tggagcgtat ggagctgatt
     5581 gccccggcgc acgttgacaa cccgtttcgt tttagcttcc gcgcgccgga aagcctgcct
     5641 aatccgttat taaagatgga aaaagtcagc gcaggctatg gtgatcgcat tattctcgac
     5701 tcgattaaac tgaatctggt ccccggctcg cgcattggtc tgctaggccg caacggcgcg
     5761 ggtaaatcga cattaatcaa actgttagcc ggcgagcttg cgccagtcag cggtgaaatt
     5821 ggtctggcga aagggatcaa gctcggctac ttcgcccagc atcaactgga atacctgcgc
     5881 gccgacgaat cgcctattca acatctggca cgtttagcgc cgcaggaact ggagcaaaaa
     5941 ctgcgtgact acctcggcgg ctttggtttc cagggcgata aagtgacgga agaaacgcgc
     6001 cgcttctccg gtggggaaaa agcccgcctg gtgctggcat taattgtctg gcagcgtccg
     6061 aatctgctgc tgctcgacga accgactaac caccttgacc tcgacatgcg tcaggcactg
     6121 actgaagcat taatcgagtt cgaaggcgcg ctggttgtcg tttcgcacga ccgtcatttg
     6181 ctgcgttcca ccactgacga tctctacctg gttcacgatc gtaaagtcga accgttcgac
     6241 ggcgatctgg aagattatca acagtggttg agcgacgtac aaaagcagga aaaccagacc
     6301 gacgaagcgc caaaagagaa cgcaaacagc gcccaggcac gtaaagatca gaagcgtcgg
     6361 gaagcggagc tgcgtgcgca aacccaaccg ctgcgtaaag agatcgcccg tctggaaaaa
     6421 gagatggaaa agctgaacgc acagctggcg caggcggaag agaaactcgg cgacagcgaa
     6481 ctgtacgatc aaagccgtaa agcggagttg accgcctgcc tgcaacagca agccagcgcc
     6541 aaatccggcc tggaagagtg cgaaatggcg tggctggaag cccaggagca gcttgagcag
     6601 atgctgctgg aaggccaaag caactgatgg cgcagataac gacgaccgat gccaatgaat
     6661 tcagcagcag tgctgaattc acccctatgc gcggctttag caattgtcat ctacaaacca
     6721 tgctgccgcg tctgtttcgt cgcaaggtga aattcacccc gcactggcag cgactggagt
     6781 tgcccgacgg cgattttgtc gatctcgcgt ggagtgaaga tcctgcacag gcgcggcata
     6841 aaccgcgttt agtcgtgttt cacgggctcg aaggcagtct caatagccct tacgcccacg
     6901 gtctggttga ggcggcgcaa aagcgcggct ggctgggcgt ggtgatgcat tttcgcggat
     6961 gcagcggtga accaaaccgt atgcaccgca tttaccattc gggtgaaacc gaagacgcca
     7021 gctggttttt acgctggctg cagcgcgaat ttggacatgc gccaacggct gccgtcggct
     7081 attcgctcgg cggtaatatg ctcgcctgtt tgctggcaaa agaaggtaat aacctcccga
     7141 ttgatgcagc ggtaattgtc tccgcgccat ttatgctgga agcctgtagt tatcatatgg
     7201 aaaagggctt ttcccgcgtt tatcagcgtt acttgctgaa cctgttaaaa gccaatgccg
     7261 cgcgcaagct ggcagcctac cccggaacac tgccgattaa tctcgcgcag ttaaaatcgg
     7321 tacgtcgcat ccgtgaattt gacgatctaa tcaccgccag aattcacggc tacgctgacg
     7381 ctatcgacta ttatcgtcag tgtagcgcca tgccgatgct caatcggatc gccaaaccga
     7441 cgctgattat tcacgccaaa gacgatccgt ttatggatca tcaggtgatc ccgaaaccgg
     7501 aaagtctccc cccgcaggtg gagtatcaac tgactgaaca tggcggtcat gttggcttta
     7561 ttggcggtac attacttcat ccgcaaatgt ggctggagtc acgcattcct gactggttaa
     7621 caacgtatct ggaggcgaaa tcatgttgat tccgtggcaa gacctctccc ccgaaacgct
     7681 ggaaaattta attgaaagct ttgtgttacg tgaaggcacc gattatggtg aacatgagcg
     7741 tacacttgaa cagaaagtcg ccgacgtcaa acgccagcta cagtgcggag aagcggtgct
     7801 ggtatggtcg gagctgcacg aaacggtcaa tatcatgccg cgcagtcagt ttcgcgaata
     7861 acctgcaacc gtcggcggac gagataataa tgatagctaa gtcatggagt tactatgtct
     7921 gccaaacatc cggtcattgc ggtaacagga tccagcggcg cggggaccac caccaccagc
     7981 ctcgcgtttc gtaaaatatt cgcgcagtta aatctgcatg cagccgaggt ggaaggcgac
     8041 agttttcacc gttacacccg cccggaaatg gacatggcga tccgcaaagc acgcgacgcc
     8101 gggcggcata tcagctactt cggccccgaa gcgaacgact tcggcctgct ggaacaaacc
     8161 ttcattgaat atggtcagag cggcaaaggg aaatctcgca aatatctgca tacctacgat
     8221 gaagccgtac cgtggaatca ggtaccgggg acattcaccc cctggcaacc tttaccggaa
     8281 cccactgatg tactgtttta tgaaggttta cacggcggcg tagtcacgcc acagcataac
     8341 gttgcgcagc atgtggactt actggtcggc gtggtgccta tcgttaacct tgagtggatt
     8401 caaaaactga tccgcgacac cagcgagcgc gggcactcac gagaagcagt gatggactcg
     8461 gtagtgcgtt caatggaaga ctatatcaac tacatcacac cgcagttttc ccgcacccat
     8521 cttaacttcc agcgcgttcc caccgtcgac acttcaaacc cgttcgcggc aaaaggtatc
     8581 ccgtcgctgg atgaaagctt tgtggtgatc cattttcgta atctggaagg gatcgatttc
     8641 ccctggctgc tggcgatgtt gcaaggctca ttcatttccc acatcaatac gttagtggta
     8701 ccgggcggca aaatgggtct ggcaatggaa ttaattatgc tgccgctggt gcaacgattg
     8761 atggaaggaa agaaaatcga gtaactttgc tattacgccg gataaaatac tatccggctt
     8821 cacaatggga tagttaaatc acgcggcaac cacttcatac gagtgagtaa tattcaccgc
     8881 tttttccagc atcaacgcca ctgaacaata tttctcggca gagagatcaa ccgcacgtgc
     8941 aaccgctgcg tctttcaggt cgcgaccggt aacgataaaa tgcagattaa tgtgcgtaaa
     9001 caggcgtggt gcctcttcgc ggcgttcaga ggtcaatttt acttcacaat cgaccacatc
     9061 ctgacgccct ttttgcagga tagaaaccac atcgatggca ctgcaacccc ccgccgccat
     9121 caacaccatt tccatcggac ttggtgcttt atcgcctgag ttgccgtcca ttaaaatctg
     9181 gtgaccagag gcggattcgc ccagaaaagt taacccttcg acccacttca ctcgcgcttg
     9241 catttttgct actccactgc gtcaattttc ctgacagagt acgcgtacta accaaatcgc
     9301 gcaacggaag gcgacctggg tcatgctgaa gcgagacacc aggagacaca aagcgaaagc
     9361 tatgctaaaa caatcaggat gctacagtaa tacattgatg tactgcatgt atgcaaagga
     9421 cgtcacatta ccgtgcagta cagttgatag ccccttccca ggtagcggga agcatatttc
     9481 ggcaatccag agacagcggc gttatctggc tctggagaaa gcttataaca gaggataacc
     9541 gcgcatggtg cttggcaaac cgcaaacaga cccgactctc gaatggttct tgtctcattg
     9601 ccacattcat aagtacccat ccaagagcac gcttattcac cagggtgaaa aagcggaaac
     9661 gctgtactac atcgttaaag gctctgtggc agtgctgatc aaagacgaag agggtaaaga
     9721 aatgatcctc tcctatctga atcagggtga ttttattggc gaactgggcc tgtttgaaga
     9781 gggccaggaa cgtagcgcat gggtacgtgc gaaaaccgcc tgtgaagtgg ctgaaatttc
     9841 gtacaaaaaa tttcgccaat tgattcaggt aaacccggac attctgatgc gtctgtctgc
     9901 acagatggcg cgtcgcctgc aagtcacttc agagaaagtg ggcaacctgg cgttcctcga
     9961 cgtgacgggc cgcattgcac agactctgct gaacctggca aaacaaccgg atgctatgac
    10021 tcacccggac ggtatgcaaa tcaaaattac ccgtcaggaa atcggtcaga ttgtcggctg
    10081 ttctcgtgaa accgtgggac gcattctgaa gatgctggaa gatcagaatc tgatctccgc
    10141 acacggtaaa accatcgtcg tttacggcac tcgttaatcc cgtcggagtg gcgcgttacc
    10201 tggtagcgcg ccattttgtt tcccccgatg tggcgcagac tgatttatca ccccgatatt
    10261 aactatgcac tgcgacaaac gctggtgcta tgtttgcccg tggccgttgg gttaatgctt
    10321 ggcgaattac gattcggcct gctcttctcc ctcgttcctg cctgttgcaa tattgcgggc
    10381 cttgatacgc ctcataaacg ttttttcaaa cgcttaatca ttggtgcgtc gctgtttgcc
    10441 acctgtagct tgctgacaca ggtgctactg gcaaaagatg ttcccctgcc ctttttgctg
    10501 accggattaa cgctggtact tggcgtcact gctgaactgg ggccattgca cgcaaaattg
    10561 cttcccgcat cgctgctcgc cgccattttt accctcagtc tggcaggata catgccggtc
    10621 tgggaaccgt tgctcatcta tgcgttgggc actctctggt acggattgtt taactggttt
    10681 tggttctgga tctggcgcga acaaccgctg cgcgagtcac taagtctgct gtaccgcgaa
    10741 ctggcagatt attgtgaagc caaatacagc ctgcttaccc agcacaccga ccctgaaaaa
    10801 gcgctgccgc cgctgcttgt gcgccagcaa aaagcggtcg atctaattac ccagtgctat
    10861 cagcaaatgc atatgctttc cgcgcaaaat aataccgatt acaagcggat gctgcgtatt
    10921 ttccaggagg cgctggattt gcaggaacat atttcggtca gtttgcatca gccggaagag
    10981 gtgcaaaaac tggtcgagcg tagccatgcg gaagaagtta tccgctggaa tgcgcaaacc
    11041 gtcgccgctc gcctgcgcgt gctggccgat gacattcttt atcatcgcct gccgacgcgt
    11101 tttacgatgg aaaagcaaat tggcgcactg gaaaaaatcg cccgccagca tccggataat
    11161 ccggttgggc aattctgcta ctggcatttc agccgcatcg cccgcgtgct gcgcacccaa
    11221 aaaccgctct atgcgcgtga cttactggcc gataaacagc ggcgaatgcc gttacttccg
    11281 gcgctgaaaa gttatctgtc actaaagtct ccggcgctac gcaatgccgg acgactcagt
    11341 gtgatgttaa gcgttgccag cctgatgggc accgcactgc atctgccgaa gtcgtactgg
    11401 atcctgatga cggtattgct ggtgacgcaa aatggctatg gcgcaacccg tctgaggatt
    11461 gtgaatcgct ccgtgggaac cgtggtcggg ttaatcattg cgggcgtggc gctgcacttt
    11521 aaaattcccg aaggttacac cctgacgttg atgctgatta ccaccctcgc cagctacctg
    11581 atattgcgca aaaactacgg ctgggcgacg gtcggtttta ctattaccgc agtgtatacc
    11641 ctgcaactgt tgtggctgaa cggtgagcag tacatccttc cgcgtcttat cgataccatt
    11701 attggttgtt taattgcttt cggcggtact gtctggctgt ggccgcagtg gcagagcggg
    11761 ttattgcgta agaacgccca tgacgcttta gaagcctatc aggacgcgat tcgcttgatt
    11821 ctgagcgagg acccgcaacc tacgccactg gcctggcagc gaatgcgggt gaatcaggcg
    11881 cataacactc tgtataactc attgaatcag gcgatgcagg aaccggcgtt taacagccat
    11941 tatctggcag atatgaaact gtgggtaacg cacagccagt ttattgttga gcatattaat
    12001 gccatgacca cgctggcgcg ggaacaccgg gcattgccac cggaactggc acaagagtat
    12061 ttacagtctt gtgaaatcgc cattcagcgt tgtcagcagc gactggagta tgacgaaccg
    12121 gggagttctg gcgatgccaa tatcatggat gcgccggaga tgcagccgca cgaaggcgcg
    12181 gcaggtacgc tggagcagca tttacaacgg gttattggtc atctgaacac catgcacacc
    12241 atttcgtcga tggcatggcg gcagcgaccg catcacggga tttggctgag tcgcaagttg
    12301 cgggattcga aggcgtaata caggctaaac catcggatga ggcgttcacg ccgcatccga
    12361 catcttttgc ctgacgcgtc ttatttacgc cccaatcacc ttcgccaccg cgtgggcgaa
    12421 acgatgcatc ccttcatcga tatccgcatc ttccaccacc agcgacggcg caaaacgcat
    12481 cacatcaggc ccggcattca gcaccattac gccttcctct gcaccggcat acaggaaatc
    12541 acgcgcccga cctttgtact gtggtttcag ctccgcgcca attaacagcc ccataccgcg
    12601 aatatcgcta aacacatcgt actgctgatc gatcttctgc aaatggtcaa caaaacgctg
    12661 gcgtttcgcc tgaatgcctt ccagcacttc aggggtattg atgatatcaa acgccgcgcc
    12721 cgctactgca caggccagag gattaccgcc gtaggtggaa ccgtgagaac caggatgaaa
    12781 tgcagaagca atttccgccg tggtcagcat ggcgctaatc gggaagccgc cgcctaacgc
    12841 tttcgcagag gtcagaatat ccggcgtcac accgtagtgc atgtaagcaa acaaatcgcc
    12901 agtccgcccc atcccgcact gcacttcatc aaacaccaat aatgcctgat gttgatcgca
    12961 cagctcgcgc aagccctgca aaaactctgg cgtcgctgcc gtcacaccgc cctcgccctg
    13021 gatcggctca accaccaccg cacaggtatg gtcatccatc accgctttca ctgcgtggag
    13081 atcgttaaaa ggcacgtgga tgatgtctgc cggttttggc ccaaagccgt cggaatattt
    13141 tggctgccca cccaccgaaa cggtaaacag cgagcgacca tgaaaagcgt tatggaaggc
    13201 aataattttg gttttgaacg ggctatgacg cacacaggcg taatggcgtg ccagtttaaa
    13261 ggcggtttcg ttagcttccg tgccggagtt cataaacacc acgcgttcgg caaaagttgc
    13321 ctcaatcagt ttacggccaa gacgcagcgc cggctcattg gtgaaaacgt tactaatatg
    13381 ccacagagtt tcgccctggg tttttaacgc gttcaccagc gcaggatggc aatggcccaa
    13441 cgccgtaact gcaatgccac ccgcgaaatc gacatactcc ttgccctgtt gatcccagat
    13501 tcggctgccc tgacctttta ccggaataaa ctctgccgga gcataaatcg gcagaatcac
    13561 ttcatcgaaa gtcgcgcgtg taattgctgt ttgttcaatt gccatctcat gatcaccctg
    13621 ttacgcataa ataaatatga aattataatc acaaaatatg cataaaaaat cactgaatgg
    13681 caatcagaaa tcagcgatgc agaaaattag ccagcagttg atgtccttgt tcgctgagaa
    13741 tactttctgg atggaattgc acaccttcca gatcccactg gcgatggcga atccccataa
    13801 tctcgcgggt ttcgctccag gccgtcactt caaagcactc cggtaatgag tcaggttcca
    13861 ccaccagcga atggtagcgt gtcacggtga gtggatttgc cagcccctta aatacgccca
    13921 caccattatg tgtaatcggc gaggttttgc cgtgcatgac ctttgcggcg cgcacaactt
    13981 tgccgccaaa tgcctgcgcc attgcctgat gaccgaggca gacgccaaga atcggcaagc
    14041 gtccggcata gtgacgaata acgtcaaggg agatcccggc ttcatctggc gtacaggggc
    14101 cgggtgagat gacaattttt tgtggtttaa gggcgtcgat atccgccaac gtcaacgcat
    14161 cgttgcgctt aaccaacaca tccgccccca gttcacaaaa gtactggtag aggttccagg
    14221 taaaagaatc gtagttatca ataagcagga tcatggcggc tccggtacga aagaaccgcg
    14281 ctattttact cagattcccc ggcttcgctt accactttgc taaatatcat ctgcagcgcg
    14341 gtgagatccc ccattgcccc attctgattt gcctgattcc aggcctcttt ttcaataccc
    14401 tgccagctca gttgatatcc ggcatgaatc gccagttgct cgaagaagat ccgctgtgcc
    14461 aaaccacttc ccacccggaa gggatgcagc acgttgattt cacaatagta atgcgccagc
    14521 cgctcgacga acttcgcttt ctccaggcca accagataac cttcttcctc cagatcctgc
    14581 atcagggcat tgccctcttt ttcgatataa gcaaagtggc agaacggcgt atcgccctga
    14641 taaatatcga cttcgcgcag ttgccccgcc cagtcgaaaa tatcctgata caactggcga
    14701 tgaagagtac gcaaatgcgg taaaccccga accaacgggc caagttcaat ggtcgcagca
    14761 cgcagcgccg tcatttcgta agcggcctgt tccagccgct gctgctggcg gatgttcagc
    14821 cggttacgca tgatatcaag gcctggataa agatacggat cgcgcccttc gccgaattta
    14881 tcgctcatag tgcctcctca gcgcttcaag acgcgctaaa gcctctgcgg cagtaagagt
    14941 gactaaaggc atctcgacgc cttcaagacg gcgactggcc tggaaattac gattacgctg
    15001 aagctcccag agacgggact tttgcttatc ggtgagtttc ttcacttgat gcctccctga
    15061 acgtgtccat ttgccacaag tataagcggc aaatccgggt tacgccggga gagcagaagc
    15121 gcgggcaaga ttgcccgcgc gagaaatcat tacggcagga ctttagcgga aaggataact
    15181 accggttttg acggcacatt ctggtacgga ccaacgtcat gagtcggcac ctgggaaatc
    15241 ttatcggcaa cgtccatgcc tttcaccact ttaccaaata ccgcgtaacc gaaatcacgc
    15301 tgaccgtggt caaggaaggc gttatcggca acgttgataa agaactggct ggtggcgctg
    15361 tctttgtcag cggtacgcgc catcgcgatg gtgccacgcg tgttgcgcag gccgttatca
    15421 gcttcatttt tgattggcgg gtttggtttt ttctgctgca tctgctcggt gaaaccgccg
    15481 ccctgaatca taaagccagg aatgacgcgg tgaaaggtcg tgttgttata aaaaccactg
    15541 ttcacataat cgacaaagtt ttgcacagac actggcgctt tttgtttatc cagctccagt
    15601 tcgatgttac cagctgaggt tgtcaacaat acgtgcgggt cccctttcgc tgccattgct
    15661 gcgggagaaa gagcagaaag agcgaaaaca gcagccatcg ccgccagggt cgatttgaac
    15721 atgagatttc cttaacaaac agagcagaaa aaaagcaagt gaagagattc taaatagcct
    15781 tcaagaacaa ggccatccct ttacctaatt ttacgtatct gaaatatatg taacctgagg
    15841 ataacttttt attccgtgat caaaatcacc tcttaaaatg caatttagca accgattgca
    15901 ataaaacatt taatcagatc acaaaatcgc ctaaaatcgc ccgctcgcgg catagcccgc
    15961 tatgccattt tatttttgaa cgcaggccaa tcatgactaa cagcaatcgc atcaagctca
    16021 catggattag ctttctttcc tacgcactga ccggtgcgtt ggttattgtc accgggatgg
    16081 tgatgggaaa tatcgccgat tatttcaatc tgcctgtttc cagcatgagt aacaccttca
    16141 ccttcctcaa tgccggcatt ttaatctcta tcttcctcaa cgcctggctg atggaaatcg
    16201 tcccgttgaa aacgcagtta cgttttggct ttctcctgat ggtgctggcg gttgccggtt
    16261 tgatgttcag ccacagcctg gcgctgttct cggcggcgat gttcattctc ggggtggtca
    16321 gcggcatcac catgtcgatt ggtacattcc tggtaacaca aatgtatgaa ggacgtcagc
    16381 gcggttcacg cctgttattt accgactcct tcttcagtat ggccgggatg attttcccaa
    16441 tgatcgccgc gtttctgctg gcacgtagca ttgagtggta ctgggtttat gcctgcatcg
    16501 ggctggtgta tgttgctatt tttattctga ccttcggctg tgaattcccg gcactgggca
    16561 aacatgcgcc aaaaacagat gctccggtag agaaagaaaa gtgggggatc ggcgtactgt
    16621 ttctctctgt tgcggcgctg tgctacatcc tcggtcagtt aggttttatc tcctgggtgc
    16681 cggaatatgc caaaggcctg ggcatgagcc tgaacgacgc aggcacgctg gtaagtaact
    16741 tctggatgtc gtacatggtc ggcatgtggg cgttcagctt tattcttcgc ttctttgatt
    16801 tgcaacgtat cctgatcgta ctggctggtc tggctgcgat tctgatgtac gtctttaaca
    16861 ccggaacacc ggcatatatg gcgtggtcaa ttctcgccct gggcttcttc tccagcgcga
    16921 tctataccac catcatcacc ctgggttcac agcagaccaa agtaccgtcg ccaaaactgg
    16981 ttaactttgt cctgacctgc gggaccatcg gtactatgtt gacctttgtg gttaccggcc
    17041 cgattgttga acatagtggt ccgcaggcgg cgctgctgac ggcaaacggt ctgtacgctg
    17101 tcgtctttgt gatgtgcttc ctgttaggtt tcgtcagccg tcaccgtcag cataacaccc
    17161 tgacctctca ttaattgttc atgccgggcg gcactatcgt cgtccggcct tttcctctct
    17221 tactctgcta cgtacatcta tttctataaa tccgttcaat ttgtctgttt tttgcacaaa
    17281 catgaaatgt cagacaattc cgtgacttaa gaaaatttat
//
LOCUS       DAZUCC010000101        17226 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZUCC010000101 DAZUCC010000000
VERSION     DAZUCC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.101"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..1121
                     /gene="allD"
                     /locus_tag="ACHY09_002821"
     CDS             72..1121
                     /gene="allD"
                     /locus_tag="ACHY09_002821"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFS3885775.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVNALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1143..2378
                     /gene="allC"
                     /locus_tag="ACHY09_002822"
     CDS             1143..2378
                     /gene="allC"
                     /locus_tag="ACHY09_002822"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFS3885776.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKQNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2389..3174
                     /gene="allE"
                     /locus_tag="ACHY09_002823"
     CDS             2389..3174
                     /gene="allE"
                     /locus_tag="ACHY09_002823"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFS3885777.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELEHIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3303..4448)
                     /gene="glxK"
                     /locus_tag="ACHY09_002824"
     CDS             complement(3303..4448)
                     /gene="glxK"
                     /locus_tag="ACHY09_002824"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFS3885778.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDAYGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4470..5771)
                     /gene="ybbY"
                     /locus_tag="ACHY09_002825"
     CDS             complement(4470..5771)
                     /gene="ybbY"
                     /locus_tag="ACHY09_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFS3885779.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTSDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5828..7189)
                     /gene="allB"
                     /locus_tag="ACHY09_002826"
     CDS             complement(5828..7189)
                     /gene="allB"
                     /locus_tag="ACHY09_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFS3885780.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7250..8704)
                     /gene="ybbW"
                     /locus_tag="ACHY09_002827"
     CDS             complement(7250..8704)
                     /gene="ybbW"
                     /locus_tag="ACHY09_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFS3885781.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLVICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFMVMRGQINLDELYTAAGDFKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTAAEKTGEQKVTG"
     gene            complement(8874..9752)
                     /gene="glxR"
                     /locus_tag="ACHY09_002828"
     CDS             complement(8874..9752)
                     /gene="glxR"
                     /locus_tag="ACHY09_002828"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFS3885782.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9852..10628)
                     /gene="hyi"
                     /locus_tag="ACHY09_002829"
     CDS             complement(9852..10628)
                     /gene="hyi"
                     /locus_tag="ACHY09_002829"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFS3885783.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHFTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(10641..12422)
                     /gene="gcl"
                     /locus_tag="ACHY09_002830"
     CDS             complement(10641..12422)
                     /gene="gcl"
                     /locus_tag="ACHY09_002830"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFS3885784.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMSVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTEACFMHYE"
     gene            complement(12512..13327)
                     /gene="allR"
                     /locus_tag="ACHY09_002831"
     CDS             complement(12512..13327)
                     /gene="allR"
                     /locus_tag="ACHY09_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFS3885785.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(13405..13887)
                     /gene="allA"
                     /locus_tag="ACHY09_002832"
     CDS             complement(13405..13887)
                     /gene="allA"
                     /locus_tag="ACHY09_002832"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFS3885786.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            14117..15043
                     /gene="allS"
                     /locus_tag="ACHY09_002833"
     CDS             14117..15043
                     /gene="allS"
                     /locus_tag="ACHY09_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFS3885787.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            15112..16206
                     /gene="mnmH"
                     /locus_tag="ACHY09_002834"
     CDS             15112..16206
                     /gene="mnmH"
                     /locus_tag="ACHY09_002834"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFS3885788.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVKGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTR"
     gene            16322..16687
                     /gene="ylbG"
                     /locus_tag="ACHY09_002835"
                     /pseudo
     CDS             16322..16687
                     /gene="ylbG"
                     /locus_tag="ACHY09_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         4507 a         4497 c         4126 g         4096 t
ORIGIN      
        1 acataatcca gatattttcc ctgtatgtta attgcagtca tgcttcacac cgtcgttaaa
       61 aaggaagaca gatgaaaatc agtcgggaaa cactccacca gctaattgag aataaactct
      121 gccaggctgg gttaaaacgt gagcacgctg caaccgtggc tgaagtattg gtttacgccg
      181 atgccagagg gatccactct catggcgcgg tgcgcgtgga atactacgcg gaacgcattt
      241 caaaaggcgg caccaaccgc gaaccggagt ttcgtcttga ggaaaccggg ccgtgctcgg
      301 caattttaca tgccgacaat gccgccggac aggtcgcggc gaaaatgggt atggaacatg
      361 ccatcaaaac cgcccagcaa aatggcgttg cggtggtcgg tatcagccgg atgggtcaca
      421 gcggcgcaat ctcttatttt gtgcagcagg cagctcgcgc cgggttaatt ggcatttcga
      481 tgtgccagtc cgatccaatg gtggtgccgt ttggcggcgc ggaaatttac tacggtacta
      541 acccactggc ctttgccgcg ccgggagaag gcgacgagat ccttaccttt gatatggcga
      601 ctaccgtaca ggcatgggga aaagtgctcg acgcccgctc gcgtaatatg tctatcccgg
      661 atacctgggc ggtcgataaa aacggagcac caacaaccga tccatttgca gttaacgccc
      721 tgctccctgc cgccggaccg aaagggtatg gcctgatgat gatgattgac gtcctctcag
      781 gcgtcttact cggcttaccg ttcgggcgac aggttagttc gatgtatgac gatttacacg
      841 ccgggcgtaa tttggggcaa ttacatatcg ttattaatcc gaactttttc tcctccagcg
      901 aattattccg tcaacatctt agccagacca tgcgcgaatt aaatgccatt acccccgcgc
      961 ccggttttaa tcaggtttat tatcccggac aggatcagga tattaaacaa cgcaaagccg
     1021 ccgtcgaagg catcgaaatt gttgatgata tttaccagta tttaatttcc gacgcgcttt
     1081 ataacacgtc atacgaaacg aaaaatccct ttgcgcaata attattgaga caggactttc
     1141 ttatgattac acatttccgt caagccatag aagaaacgct gccctggctt tcctcttttg
     1201 gcgctgaccc agcgggtggg atgacccgtt tactttattc gccggaatgg ctggaaactc
     1261 agcagcagtt taaaaaaaga atggcagcaa gcgggctgga aacacgtttc gatgaagtgg
     1321 ggaatttata cggtcgcctg agtggcaccg aatatccaca ggaagtggtt ctgagcggtt
     1381 cgcatatcga taccgtggtt aacggcggta acctcgacgg gcaattcggt gcgctggcgg
     1441 cgtggctggc aattgactgg ctgaaaacgc aatacggcgc gccgttacgt acggtcgaag
     1501 tggtggcgat ggcagaagaa gaaggcagcc gcttcccgta tgtcttctgg ggcagtaaaa
     1561 atatctttgg gctggcgaat cctgacgacg tgcggaatat ctgtgatgcc aaaggaaata
     1621 gttttgtcga tgcgatgaag gcttgcggat ttactctgcc gaacgcccca ctaactccgc
     1681 gtcaggatat taaagccttt gtcgaactgc atatcgaaca gggctgtgtg ctggaaagta
     1741 atgggcagtc aattggcgtg gtgaatgcaa ttgtcgggca gcgtcgttat acggtgacgc
     1801 tgaacggcga atcaaaccat gcaggcacca cgccgatggg ttatcgtcgt gatacggttt
     1861 acgctttcag tcgcatttgc catcagtcga tcgaaaaagc gaaaaagatg ggcgacccgc
     1921 tggtgcttac ctttggcaag gtggagccgc gtccgaatac ggtgaatgtg gtaccgggta
     1981 aaaccacgtt caccattgat tgtcgtcata ccgacgctgc cgtgctgcgc gatttcaccc
     2041 agcagttaga aaacgacatg cgggcgattt gcgatgaaat ggacattggt attgatatcg
     2101 atttatggat ggacgaagaa cccgtgccga tgaataagga gctggtcgcc accctgacag
     2161 aattgtgtga aagtgaaaaa cagaactacc gggtaatgca cagtggtgcc gggcacgacg
     2221 cgcaaatttt cgcgcctcgc gtgccgacct gcatgatttt cattcccagc atcaacggga
     2281 tcagccataa cccggcggaa cgcaccaata ttaccgacct tgccgaaggg gtcaaaacgt
     2341 tggcactcat gctttatcaa cttgcctggc agaaataagg agttataaat gggatattta
     2401 aataacgtca ccggttaccg cgatgattta ctggctaacc gtgcgattgt gaaacacggt
     2461 aatttcgcac tgttaacacc agacggtctg gtaaaaaata ttattccggg ctttgaaaat
     2521 tgtgacgcga caatcctctc cacgccaaag ctgggtgcct cttttgttga ttatctggtc
     2581 acactgcatc aaaacggcgg caaccaacag ggcttcggtg gcgaaggcat tgaaacgttc
     2641 ctgtatgtga tctctggaaa tatcactgcc aaagccgaag ggaaaacatt tgccttaagc
     2701 gaaggtggct atctttattg cccgccaggc tccttaatga cgtttgttaa cgcccaggcc
     2761 gaagacagcc aaatcttttt atataagcgt cgctatattc cggtagaagg tcatgcgccg
     2821 tggctggttt ctggcaatgc cagcgaactg gaacacattc attatgaagg catggacgat
     2881 gttattctgc tggattttct gcccaaagag ttaggttttg atatgaacat gcatatcctc
     2941 tcttttgcgc caggtgccag ccacggttat atcgaaactc acgtccagga acacggtgcc
     3001 tatattcttt ccggtcaggg ggtttataac ctcgacaata actggatccc ggtgaaaaaa
     3061 ggcgattaca tctttatggg cgcttactcc ttacaggccg gttatggcgt agggcgtggt
     3121 gaagcgttta gttatattta ctcaaaagat tgcaaccgcg acgtagagat ttaagtcata
     3181 ccactcgcct gattattttt agctatgttg gattttgcca cggtttattc cgtggctttt
     3241 tttgtagtca attcaggtaa aaaaagatgc gatataaatc gcatccgctt taaaggtaag
     3301 agtcagtttt taattccctg acctatttta atggcgcagg caatatttcg cgcgctgtta
     3361 aagagattgg tttcaccgct ggcgagcact tcggctagag gtgccagacg tggcaaaatg
     3421 ctgaataccg cgtcaatgcc gtactggtgc accacttcca cgccatcacc caatacgcca
     3481 gcaatcccaa tcaccgggac attaaactgc ttcgccaccg acgccacgcc cagcggcgct
     3541 ttaccacctg ccgtttgcga gtcgatgcgc ccttccccgg tgatcaccag tgctgcgccc
     3601 tgcactgcct gcgcaagatt gaccgcattc aacacaatct caatacccgg tttaatatcc
     3661 gcattgagga ataccgccgc cgcaatgccc ataccgcccg ccgcgccacc acccgccatc
     3721 tggcagacgt tgatttcagt ttgctgttgt aaaacacggg cgtaattttg cagcccctgc
     3781 tccagctctt cgaccatttc cggcgttgcc cctttttgcg ggccgaacac cgccgccgcg
     3841 ccgcgtgccc ctaccagcgg attatcaaca tcacatgcga cttcaatatg gcagttcgcc
     3901 agacgcggat cgcattcatc catctcaata cttgccacgc gtgctaaatt gccgccgtta
     3961 gccgccagca cctgaccgta ggcatcaagg aaacgcacac cgagcgcctg cgccatgccc
     4021 ataccgccgt caaccgtcgc gctaccacca atgccgagaa taatatggcg aatgccgtta
     4081 tccagcgcat ggcgaattaa ctcccccgta ccaaaactgg aggccagcaa cggattacgc
     4141 ttttcaggcg cgaccagcat caggccactt gctgctgcca tctcaatcac cgccgttttc
     4201 ccgtcgccgg taatgccata aaaagcattc actttttcgc ccatcggccc gcagacttca
     4261 agcttcacga tgttaccgtc cgtcgcggcg accatcgcct ccaccgtccc ttcgccgcca
     4321 tccgctatcg gcaaacagat atagtgcgca tcgggaaaga gggtcgaaaa cccggcttta
     4381 attgcctgac aacatttttc tgcacttaag ctctctttaa aagagtctgg cgcaatgaca
     4441 atcttcatta tcaatccttt ttcaacaaat tattcgatgc gttcccacgg aataagatta
     4501 tccataagaa cagccagtaa aattccgacc agtaagccgt tactgagcag aggacgaagc
     4561 gttaatggaa ggtcttgcag atacacaggc ggtaatgcca ttaaaaatat gccgacaaat
     4621 aacggcaatg cgagtcgata aatattgcga gcggtaaacg tgatttgctg gctaaacacc
     4681 agcgcggaaa agagtaaagg cagataagaa accagcatga ccgcgctact cactggtaaa
     4741 gggatactgc aaaacagtcg cgtgagtgcc ggaaccagtg ccaccagcag gcaaataacg
     4801 ctgccataaa taaacgaacg ccgcgtgtaa tcgctagtct gggttaataa accaatggat
     4861 gaaacaaacg gtgaaaatgg aattactgcc agtggcacgg ttatcagtgt cataaatccg
     4921 gtcgccacaa agctgcgacg ataacgcgta ttccccgcgc cctgctgcgg ataaaaaaca
     4981 tccgtgcccc gaatcgcacc gtaggtattg ctgatattta ccagacctgt aatcaccgcc
     5041 gtcagaataa tccccggcga caaagcacca ccactgccga gcgggaacca ctgccagtgc
     5101 aactcaccgg agagcgaatg cgaagaagga aagcaaaagt accacaacaa ccagccggtt
     5161 atcgtaccga ccagcaggcc gtaacgggca aaacgttgtg gcaggaaaat aatcatcgcc
     5221 agtaccaggc acatcaccgc gaccgagagc gcgaacggcg gtaattgaat tttaaaattc
     5281 gggtcggcta tgccaaacgg cagcccgagc atacctttga aaaagatagt ggtcagttgc
     5341 gcacccagca tcaacataaa caagaccatc accgacggcg taaataaccg tgccaggcga
     5401 tgtcctaatc cgctaaaacc aatcaacatc gtcagcacgc cggagagcgc aattcccacc
     5461 gccagactgg tagcgatatc gttgatcggt gtcccgcgcg atgcttcgcc aagggtgata
     5521 gtcaggatgg ttccccacca caggccgccc ggcccctcca taatagcgcg acgatgaccg
     5581 cagaacgcct gagcgaagca ggccagtgcg gtagcaagaa aagcatattg cgtgagcgta
     5641 agcaggctac tttgcggcaa ctgaaaagca gaaagtagcg taggaggaac cacaaccgtg
     5701 ttgcaaaaaa taaagaaaaa ccactgaaat cctgataaca ggctttcgcg gctgactgca
     5761 aaattgaaca taaaaacacc ttaaccggag aatgcccgcc gcaaggacgg gcattgcagg
     5821 ggccagatta ctgctgatgt ttaaggataa attgaccttt cggcgcaaca gggaagccct
     5881 gttcgatgtc gtaaatcaca tcaccacgta agatggtttt cgtgatacgc gcgccaatgg
     5941 tacggccaac atacgggctg actttgtggc gatattccag atcgtcattg gtaagaacat
     6001 agctgctatt cggctgaatg aagacgaagt cggcatcttt tcctggggcg atacggcctt
     6061 tttgctgcag accgaaaata tctgctgcgt tagtcgccat taatttgccg aacatcggca
     6121 gagacatacc gcgtttctgt accgcttcat cgaacatcac gtccatgcag ctttgcagac
     6181 cggcgatacc accccatgct ttcatgatat tgccggcttt catttccggc ggacatggag
     6241 agtggtcgga aaccaggcag tcgatttcac cgttaaacag tttttcccac atgcctttct
     6301 ggttttccag atcgcggatc ggcggtgaac acttcgccag agtaccgatt tcttcgaact
     6361 gatcggtatc aagtacaaag taatgcgggc aggattcaca agtaacgtcc tgaccttcct
     6421 gacgtgcacg agtcacttct tcaacgcctt ccgggctgct gatatggcaa acgtgcagac
     6481 ggcaaccggc aactttcgcc agatacagca cgcggcgaat cgcttccact tcggtaaata
     6541 ccggacgcga agccacatag tcatgagcag ttacgcgacc ttcacgcttc gcttcttcgc
     6601 ccagttcgtc acagatcagc gcgttttcgc agtgcaccag caccggctgc cccagttcgc
     6661 ccagcttctg cgcacctttg aagaactgcc agtcgtttac atcacggaag tcgttgtcga
     6721 taccgcgatc gccacaggtc gcaacgaagc atttgaagcc gacaacgccc acttcatcca
     6781 gctcatgcag acggtcgatg ttgtaagaca ccaggccacc gagttgtgcc gcatcgatag
     6841 tcagcttgcc tttagcggca tcgaacttca gttcaattga agcgcggtca accgttgctg
     6901 gcagctggtt gagcggcatt tcgatcatgg tggtgatacc accttttgcc gctgcgcgag
     6961 taccagtttc ataaccttcc cagtggctac gacccggctc agaaatatgg gtgtgcgcat
     7021 caaccatgcc tggagaaacc accagaccag acgcatccat cacttctttt gcatcgccca
     7081 gatcctgacc gatagcagca atttttccgc ctttaacggc gatatctaca acgcgagctt
     7141 cgttttctaa aataacggtg ccgtttttaa tgattaaatc aaaagacata acaaactcct
     7201 tgtaaaatac agattggttt tttaactcaa tgttcaatat cgggattaat taacctgtga
     7261 ctttttgttc tcctgttttt tctgctgctg tacgtttttt taataacgcg taggcagcga
     7321 atgcgacaat aacgccgaca aaccatgaaa cgcgtgataa aggctccatg aacggaataa
     7381 atttaccacc gagagataaa ataacggcga ccaaagttac tgagaacgcg gtaaggttaa
     7441 agccgttatc gtaatatttg aaatcacctg ctgcggtata cagttcatca agattaattt
     7501 gtccgcgcat caccataaaa taatgcgcca tcatgacacc aattaccgga cccagcattc
     7561 cgccgataat atcgaggaac agataaatac tgtcctgatt ttccattaat ttccacgggc
     7621 agatcaccag gctgataata ctggcaatca gtacgccgtt tttataggtc agtttcgtcg
     7681 gtgcaatagc ggcaatctga taaccggccg gaataatatt accggtggcg ttagtggaaa
     7741 tggtcgtcat cagaataacc agtaccgcaa agaacgaggc gaacaggctg tcccaacgct
     7801 gaacaatatc cagcacgttc caggtgtcag cgccgtaatg aatactggct ccggcaataa
     7861 tacatacccc ggcgaccgca aacagaatat aggccacaac taaacccagc gtttgcccca
     7921 gcgcctgctc acgaaacgag tgggcgtttt gcgtaaagtc ggatgcgctc accgccggtg
     7981 ccgcccagac cgcaaccacc gcgtttatca ccaccagaaa caggaagcca ctgttttctg
     8041 ctttctgaat accgcccgga atgtagtcaa agattggacc gatcccgacc agcgaaatcg
     8101 cccaaatcgc cataccgccg aaaacgatat agatgcacgg gttaagaatg gcagtgaatt
     8161 tatttaaaac ttttccgccg ccaaaaccaa taccaacgtt aaccagccag aagagtaaaa
     8221 aagtaattaa tcccggtaga gaaaggccta acagcgtgaa atcgccaccg agagtcaaaa
     8281 atcccggcca gattttgcca atcagaatca agcaggccag tgaccccgcg taacattgca
     8341 ggccaaacca cataatggca gcaataccgc ctcttaataa tccgggaaac agtgcaccac
     8401 gtacgccgta agaagcacgc aggatcatgg caaacggtac gccgtattta ctgcccgcag
     8461 caccgtttaa taccattacc gcggcaatga aaaaggcact gaggataatt gccagcataa
     8521 tactaaaggt agacaagccg agaataaaaa agccgccaac catcacataa ttgggaacgt
     8581 tatgaaccga acccatccat aaggtaaaat agttaaatgt tttccaggtc cgctgacttt
     8641 gcgttttcgg cagtagatct tcgctatagc cgcgttgctg gaatagtttt ctctgatgtt
     8701 ccataaatcc ctcttaattt cacgtatcga ataacgttgt tttaatctag ctatgaggaa
     8761 caaataaaat aaaaatatta tttttaatac tgaagcaaca tctttggctg ctttcttttt
     8821 ttaatattgc tggccgattc attttctgat atcggccatt tttattgcgg gtatcaggcc
     8881 agtttatggt tagccattaa ttccagcgcc tgcactaatg cagagtgatc caactggctg
     8941 ccaccgtttg ccgcacaggt attaaacaat tcctggcagg tcgcggtgtt tggcaggttc
     9001 agcgccagtg ctttcgcact ttgcagtgcc aggttgagat ctttctggtg cagcgcaatt
     9061 ttgaaacctg gattaaaggt gcgtttaatc atgcgctcac cgtgaacttc cagaatacgt
     9121 gaggaagcaa agccgcccat cagcgcctgg cgcacacgta ccgggtccgc accggctttt
     9181 gaagcaaaca acaaggcttc agaaaccgct tcaatattga gcgccacgat aatctgattt
     9241 gccactttgc aggtttgacc gtcgccgtta ccgcccacga gagtgatatt tttaccgagc
     9301 agttcaaaca gcggttttac acgttcaaat actgcttcat caccgccaac cataatcgat
     9361 aacgtccctt cacgcgcgcc gatttcaccg ccggagactg gcgcatcgag ataatcgccg
     9421 cccagttcat tcacctgacg agcgaaacgt ttagtttcaa tcggggaaat ggagctcata
     9481 tcaacaatgg ttttgccctt cagcgaggct ttggtacaac cattttcacc aaacagaacg
     9541 tcttcaacct gtggcgtatc cggcaccata ataaaaatga tgtccgatgc atctgttacc
     9601 tggcgcgcag tttcaacact aacggcgccc agtgacagta attcatcagc aaccggacca
     9661 atggttgtga catgcagttg atgaccggca cgcgccagat taatggccat cggtgtaccc
     9721 ataatgccta agccaataaa tcccagtttc ataattaacc tcttttaaat ttcgcttttc
     9781 ctgaattcag acaacacgat cccctgccaa cttagcggcc taaaaaagca ttgtctgaat
     9841 agcgttacgt tttaacggta cggatccatc cagcgtaaac cggcttccgt ggtggtttgg
     9901 ggtttatatt cacagccaac ccaaccgttg tagtcagaat tttcaattac cgtaaagaga
     9961 tattcgtaat taatttctcc ggttcccggt tcgccgcgat gcggattatc agcaatttgc
    10021 aggtgaccaa ttttatcggc ccactgagtc atggtattag ttaattcacc ttccatccgc
    10081 tgcatgtgat aaatgtcata ctgaattttc aaattgcagc aaccaacatc gtcaatcagt
    10141 ttcagtgcct gccgggttcc ggtgaaatgg aaaccaggaa tatcaaaatg gttaataggt
    10201 tcaatcagta ataaaatatc ttctttcatc agcatattcg cagcataacg caggttttct
    10261 acaagcgttg tgtgaatctg ttcactgctg aaaccagtcg gcgttttacc gaccagacag
    10321 ttaatttttt tattacccag agcacgagca taacgaatcg ctgctgctac gccatcccga
    10381 aactcttctt cacggccagg aatacaggca ataccccgct cgcccgccgc ccagtcacct
    10441 gccggtaaat tgtgcagcgt atgttcgagt ttattactcg ccagcacctg ttttaattct
    10501 tcaatgtcgt agtcataagg gaacataaat tcaacgccgc gaaaaccaca ctgcgcagct
    10561 ttctcaaaac gggcgagaaa atcatattca ccaaataaca tcgataaatt agcagagaaa
    10621 cgtaacatta tttatctccc ttattcatag tgcatgaaac aggcttcagt cggtgcatcc
    10681 gctgcgttat cggcgatatc ttcaaattcc atgacgttat ccagttcact gcccatcgaa
    10741 atattggtca cacgctcgag aataacttcc acgactaccg gtacccgata ttgcgccatt
    10801 aagattttcg cctgttcaaa ggctggtgca atatcttccg gtttgaaaac gcgaattgct
    10861 ttacaaccta aaccttccgc tacttttacg tggtcgacgc cataaccgtt cacttcgctg
    10921 gagttaatat tttcgaacgc gagctgtacg cagtaatcca tatcaaaggc acgttgcgac
    10981 tggcgaatca gacccagata ggcgttattg accagtacat ggatgtacgg aatgttgaac
    11041 tgtgcgccaa cggctaattc ttcaatcagg aactggaagt cgaagtcacc ggaaatcgcc
    11101 accacattgc gctctggatc agcggcacaa acccccagcg ccgccggaat cgtccagccc
    11161 agcgggcctg cctgcccaca gttgatccag tggcggtctt taaagacatg cagcatttgt
    11221 gccgcagcga tttgtgacag gccaatggtg gtgacataac aaacatcgcg gccaaaggct
    11281 ttgttcatct cttcatacac gcgctgcggt ttcaccggca cgttgtcgaa gtgggttttg
    11341 cgcagcaaag tgcgtttgcg ctgctggcag tcggcgaccc attctttacg acacggcaga
    11401 cgacccgctt tttgcatctc ctgcgccact tcaaccagca gcgtcagcgc cgctttagca
    11461 tcagagacaa tgccgagatc cggacacagc acgcggccaa tttgcgtcgg ctcaatatcg
    11521 atatgaacga ttttgcgccc ttcggtgtat ttctctaccg aaccggtatg acggttagca
    11581 aaacggttac cgataccaaa caccatgtcg gacgccagca gcgttgcgtt accgtaacga
    11641 tgcgcggttt gcagacccac catcccggcc atcagttcat gatcgtcggg aatacagccc
    11701 cagcccatca gcgttgggat caccggaatg ctggtcagtt cagcaaactg ttgtaacagt
    11761 gcagctgcgt cagcattgat taccccgccc ccggcaacaa tcaccggacg ttcggcctgg
    11821 attaacattt ctaccgcttt ttcgatctgc atacggctgg cagcaggttt gtagaccggc
    11881 agcggttcgt acatgtcagg atcaaactcg atttccgcaa cctgaacgtc gaacggtaaa
    11941 tccaccagta ccggacccgg acgaccagaa cgcatcaggt gaaatgcctg ttgcagcacg
    12001 cgaggcacca gcgccgcttc acgtactgta accgacattt tgctgaccgg tttagcaatt
    12061 gcttcaatat caacggcctg gaaatcttct ttatgcagac gggcgcgcgg agcctggccg
    12121 gtaatacaca gaataggaat ggaatcagca gaagcagaat agagcgcggt gatcatgtca
    12181 gtgcctgctg gtcccgaggt tcccagacat acgccgatat tccctgccgt tgcgcgggta
    12241 taaccttccg ccatgtgcga agcaccttcc acatgacgcg ccagaatgtg acgaataccg
    12301 ccgtgcttac gcatcgctga atagaaagga ttgattgctg ctcccggaac gccgaaggcg
    12361 gtggtgatac cttctttctc cagcacatac attgccgcgt caacggctct catttttgcc
    12421 attttattcc tacctctatt aattggaaaa attttccaac tttcaaattt tgtcaaaaaa
    12481 acccgctccg cagagcggga tgcgacagac actatggatg tgctttcagt cccaacgccg
    12541 tgctgatatc gcgggcggtg tctctgacca gctcaccctg actgacaaaa cgatcttctg
    12601 tcagtcttga tgaaggcccg gagatggaga tagcggcaac aacactacct acatcatcgt
    12661 aaattgctga agctatgcaa ttcaggccga caacatgctc ttctttatct acggtatagc
    12721 ccagttcacg cgcttgttcc aggtccttca gcaaggtggg catatccaca agcgtagttg
    12781 gcgtaaactg ctgcaaaccg gtttgcagaa tgatgctcat caactcctct tccgccagcg
    12841 gataaagcag cgccttgccc gcaccggaag catgcagcgg cagacgactg cccagcggcg
    12901 cacacatcct gaccatcgat ttacactcta actgaccaat taataccgct tcattgccgt
    12961 tacggatcgc gacattgacc gtttcgccgg aaagtaacat caggcggcgc ataaacggcc
    13021 cggcgacgga gagtacatcg cggttatgga tgtacgctgc accgacgtta aagacaccta
    13081 atcctatatg ccaccagcct aactgactgt cctgatagac aaaatccgct gcctgtaaaa
    13141 ccttcagcaa gcgaaaggtc gtggagagcg gcaaatccag attgagagaa atatcgctaa
    13201 ccgacgaact tcccccactt ttttccagat attgcagaat cgcaattccc cgctctaacg
    13261 cctgtgcgcc cttctgtgcc acaggctccg cctgtcctgg cctgccgcgc cgtctaactt
    13321 ccgtcatacc tacgttaact ccgacctaca agtgagcata aacaatgaac aattcgttta
    13381 tcttatgcaa gtcggttgca ggcgtcacgc aaaacagagt tcctgttcag gaatactttc
    13441 aacatcacag ttgtcactgc cgccgcgatc gatggtcaga aaatcggtga cgcgctgcca
    13501 ggcgaaaagt gggtgatgcc agacgttacg atggtaattc accccctgtt caccgttggt
    13561 aataaacgcc cgcagcgttg acaggtctgg tttgtcgtca cctaacgcca cgactaccac
    13621 aaacacttca cctttcatcg ggataaacgc ctgtgtaccc aacggatgac gttcgagttc
    13681 gtgaatagtc agcggcagat tcgccggttg cgcgcggtta atgctgataa gcgtacggtc
    13741 ttgctcaaga atctcaacca gcgccaaatc gtggtaacgc tctaccaggc cattgttaat
    13801 atggaaaaaa tcccgttgct gcgtttcgat tacgtcgcca taagcactaa aggcttcctg
    13861 acttaacggt aatacctgaa gtttcatctt tttgtccttg taacataatt ccgttcaacc
    13921 accgccgacc ggtcagcttg gtttcaataa caggcaatct aaaaccttaa gagaacaaca
    13981 tcaatacaaa agttggaata tttttccaaa actagattta gtccacaaat tgaacagggt
    14041 tatcatgtaa aaatactgat aaatcagagg ataagaaaat cacgcaactt gcggatttaa
    14101 aaggggatac ctgaagatgt tcgatccaga aaccttgcgg actttcattg cggttgctga
    14161 aacaggaagt ttttcaaaag cggcagaacg attatgtaaa accacggcga cgatcagtta
    14221 tcgcattaaa cttctggaag agaataccgg agtagcgctg tttttccgta cgactcgtag
    14281 cgtgacgttg acggcggctg gcgagcatct actttgtcag gccagagact ggctgagctg
    14341 gctggaaagt atgcccagcg agctgcaaca ggtgaatgat ggcgtggaac gccaggtgaa
    14401 tattgtcatc aacaacctgc tctataaccc ccaggccgtc gcccagttgc tggcgtggct
    14461 gaatgaacgt taccccttta cccagtttca catctcccga caaatctata tgggcgtctg
    14521 ggattcgcta ttatacgaag gtttttcact ggctatcggc gtcacgggaa ccgaggcgct
    14581 ggcaaatacc tttagtcttg atcccttagg atcggtgcaa tggcgctttg tcatggcggc
    14641 ggatcatccg ctggcgaacg ttgaagagcc gctaacagaa gcacagttgc ggcgctttcc
    14701 ggcggttaat attgaagaca gcgcccgcac actaaccaaa cgcgtcgcct ggcgattgcc
    14761 ggggcaaaaa gagattattg ttcccgatat ggaaacgaaa atcgccgccc atctggcggg
    14821 cgttggcatt ggttttttgc caaaatcact ttgccagtca atgatcgata atcaacaact
    14881 ggtcagccgg gtaatcccaa cgatgcgccc tccttcacca ttgagtctgg catggcgcaa
    14941 atttggcagc ggcaaagcga tagaagatat tgtgaccttg tttacccagc gcaggccgga
    15001 aatcagcgga tttttagaaa ttttcggcaa cccacgcagt taaaaatcaa gtttccttgc
    15061 gcgttggcgc gtaaaatagc gcgcaagctg atttgaggtt cactttttac tatgcaagag
    15121 agacacacgg aacaggacta tcgtgccctg ctgattgctg atacgcccat tattgatgtt
    15181 cgcgccccca tcgagtttga gcaaggcgca atgcctgccg ctatcaacct gccgttaatg
    15241 aataacgatg aacgcgccgc cgttggcacc tgctataaac agcaagggtc agacgcggcg
    15301 ctggcgctgg gtcataaact ggtggcgggt gaaattcgtc agcagcgtat ggacgcctgg
    15361 cgggcagcgt gcctgcaaaa tccgcaaggt attctctgct gcgcccgtgg cggtcagcgc
    15421 tcacatattg tgcaaagctg gttgcatgca gcggggattg attatccgct ggtgaaaggc
    15481 ggttataagg cactgcgcca gaccgcgatt caggcgacta ttgaactggc acaaaaaccg
    15541 atagtgctga ttggcggttg taccggcagc ggtaaaacgc tgttagtgca gcaacagccg
    15601 aacggtgttg atctggaagg gttggcgcgt catcgcggtt cggcgtttgg tcgcacctta
    15661 caaccacaac ttagccaggc gagttttgaa aacctgctgg ctgccgaaat gctaaaaacc
    15721 gacgcccgtc agaatttgcg cctgtgggtg ctggaagatg aaagccggat gatcggttcg
    15781 aatcacctgc cggaatgcct gcgcgagcga atgacccagg cggcgattgc ggtagtagaa
    15841 gatccgtttg agatccgtct tgagcggctg aacgaagaat atttcctgcg tatgcatcat
    15901 gattttaccc acgcgtacgg cgacgaacag ggctggcagg agtattgcga atacctgcat
    15961 cacggacttt cggcgattaa gcgtcggctg gggctacagc gctataacga actggctgca
    16021 aggctggatg cggcgctgac aacgcaactt accaccggca gcaccgacgg tcatctggcc
    16081 tggctggtgc cgttacttga agaatattac gacccgatgt atcgctatca gcttgagaaa
    16141 aaagcggaaa aagttgtctt tcgcggtgag tgggcggaag tggcggaatg ggttaagacg
    16201 cgataatgag ggacgtattt gtattagtcg cgacttgatc tgacacatgg ccttgaaagg
    16261 ttgagagtta ccggttttga tatgagtgtc gaatccttat ccaaaacaag aggtaactct
    16321 catgcttcat actgccaatc ccgtcatcaa acacaaagcc ggtttgctca atctggctga
    16381 agaactcagc aacgtatcaa aagcctgtaa aatcatgggc gtttcgcgtg atacgtttta
    16441 tcgttaccgc gaactggccg ctgaaggcgg tgtggatgcg cagattaatc gtagtcgccg
    16501 cgctcctaac cttaagaacc gtaccgatga ggcaattgaa caggctgttg ttgattacgc
    16561 cgtcgctttc ccgacacatg gtcagcaccg ggccagcaac gagctgcgta aacagggggt
    16621 ttttatctcc gacagtggtg tccgttctgt ctggttactc cataaccttg agaatctcaa
    16681 aagacgatac tgaacaaatt ttatcaggtg acgttccgta aaaagttgta tggagttctc
    16741 gatgcagtcg gcgcgactat cagtaccccc gctgaaccct ggactatcgg gccgccgtac
    16801 tggggcatgt caccctggcg tgccgctggt tttccgctaa tggtttattt cctctttctt
    16861 tcccggcgag aatttatttt aatccggaag gattatttat tttcagatag ggactcacat
    16921 taaacacgta acattttaat taatgcatgg ctgaatatgt attacacaat gtcagatatc
    16981 gtaaaagacc agtgcaattc tatgtaaact ctctggctgt tcattttatt tgttgtttca
    17041 gggtcggaac tcaccctcaa taaacaactg aatacgagta gatattcatc gtctgagcta
    17101 tatggcttta cacaatagcc gacatgtctt ttctcagtca gggattatac ttttcctcac
    17161 tacgaagttg acgacttcga tatgggatag actcttaatt caagcaatta ctctgcacaa
    17221 gcatca
//
LOCUS       DAZUCC010000102        17179 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZUCC010000102 DAZUCC010000000
VERSION     DAZUCC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.102"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..793
                     /locus_tag="ACHY09_002836"
     CDS             <1..793
                     /locus_tag="ACHY09_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFS3885789.1"
                     /translation="PASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTSVVP
                     RISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQFNG
                     NMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPRQGP
                     ASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPGTTIWQHGDDLLVRS
                     RAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            797..2128
                     /gene="traO"
                     /locus_tag="ACHY09_002837"
     CDS             797..2128
                     /gene="traO"
                     /locus_tag="ACHY09_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFS3885790.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     NNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            2125..2838
                     /gene="traP"
                     /locus_tag="ACHY09_002838"
     CDS             2125..2838
                     /gene="traP"
                     /locus_tag="ACHY09_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFS3885791.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCLLIIL
                     AAVWYKFLRAPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            2835..3365
                     /gene="traQ"
                     /locus_tag="ACHY09_002839"
     CDS             2835..3365
                     /gene="traQ"
                     /locus_tag="ACHY09_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFS3885792.1"
                     /translation="MMRIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            3412..3810
                     /locus_tag="ACHY09_002840"
     CDS             3412..3810
                     /locus_tag="ACHY09_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFS3885793.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIAQVIGLVLFIAGLLGLKQIGKKPGAGLACIL
                     SIVVGACLAVVPEMMSSGQKTLGLSPVSIS"
     gene            3867..4118
                     /locus_tag="ACHY09_002841"
     CDS             3867..4118
                     /locus_tag="ACHY09_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFS3885794.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            4033..4851
                     /locus_tag="ACHY09_002842"
     CDS             4033..4851
                     /locus_tag="ACHY09_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFS3885795.1"
                     /translation="MNKTPPLIAPARERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILMPDSFPDKKYVM"
     gene            4906..5265
                     /locus_tag="ACHY09_002843"
     CDS             4906..5265
                     /locus_tag="ACHY09_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885796.1"
                     /translation="MDTLTAGYQFSADAFRRFIIRTLTDDIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            5265..5645
                     /locus_tag="ACHY09_002844"
     CDS             5265..5645
                     /locus_tag="ACHY09_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885797.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     RNERRKCIVVHINKITYPPVNSLR"
     gene            5821..8865
                     /locus_tag="ACHY09_002845"
     CDS             5821..8865
                     /locus_tag="ACHY09_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS3885798.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRNEIEVMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            8865..9482
                     /locus_tag="ACHY09_002846"
     CDS             8865..9482
                     /locus_tag="ACHY09_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFS3885799.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWQDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            9443..10648
                     /gene="traW"
                     /locus_tag="ACHY09_002847"
     CDS             9443..10648
                     /gene="traW"
                     /locus_tag="ACHY09_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFS3885800.1"
                     /translation="MQRQPATTLLALLIGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPIREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            10645..11214
                     /gene="traX"
                     /locus_tag="ACHY09_002848"
     CDS             10645..11214
                     /gene="traX"
                     /locus_tag="ACHY09_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFS3885801.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            11287..13446
                     /locus_tag="ACHY09_002849"
     CDS             11287..13446
                     /locus_tag="ACHY09_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFS3885802.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     YYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTTASSTEYRTAEAAEPKSIIMKVWSGVNFADKIATSLSGTGTTSD
                     QLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSIFGRLVNAVSGLSDAAKAF
                     LEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTNQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFGAALVNV
                     QADSLTGLFSIAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNIRRTPGVREEKLKNINGDDKDGIKQ"
     gene            13528..14175
                     /gene="excA"
                     /locus_tag="ACHY09_002850"
     CDS             13528..14175
                     /gene="excA"
                     /locus_tag="ACHY09_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFS3885803.1"
                     /translation="MNTTKSRYPSWWAYYQLARNTYFIFGIPCLLFYAFFGTLIVHDS
                     SLSQTRDFIFAYGCWALIILPLLWLYLKTRAKKKKVQQVVQQIKESSPFAPTNDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISARTLANTIHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(14489..14641)
                     /locus_tag="ACHY09_002851"
     CDS             complement(14489..14641)
                     /locus_tag="ACHY09_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3885804.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            14974..15216
                     /locus_tag="ACHY09_002852"
     CDS             14974..15216
                     /locus_tag="ACHY09_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885805.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(15309..16844)
                     /locus_tag="ACHY09_002853"
     CDS             complement(15309..16844)
                     /locus_tag="ACHY09_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS3885806.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNALGNVLILFLMMLLGRRNVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQ
                     TNINEAGEFITGIPVLYFIVSLVIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKHFLKEGSSSSLRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHTNPCDRLFNDAQPFRELFGHKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
BASE COUNT         4277 a         4831 c         4294 g         3777 t
ORIGIN      
        1 acctgcctct ccccttgttc aggaagccgc atcactggat tcccccctga atgcaggtga
       61 aattcgggaa ctgcggggac gaatggccga taacgaacgg gccatcagcg cacctgtcac
      121 cagtgtggtg ccccgtatca gctccctcac ggtaaatctg tcacccgggg ccagtctgcc
      181 gcttgtccga accgcactgc ataacgtcag cgtggtaacg ttcacggaca tcaacggctc
      241 cccgtggcct cagtcggatc cgccttataa cgcggcaccc ggtcactttg atgtgcagtt
      301 caacggcaat atggtctccg tcactcccct gcgcgcctgg gctgcaggga atgtaacggt
      361 ctacctcaaa ggactgtctg taccggtcat tctgaatatc accagtggtg aaaccgacac
      421 caataccctg agtcaggaga tcgacagccg tctggatctg cgcattcccc gtcagggacc
      481 tgccagtcct gtggtagccg taccatcaga gaagattgcc ctgcatgatg ccaccctgca
      541 ggcctttctg gacggtgttc ctcccgatga tcccaccgtg cgtcggctga aattctccgg
      601 aaatgttccg ggcaccacca tctggcagca cggcgatgat ctgctcgtgc gatcgcgggc
      661 catgctgcgg gatgaatttg aacagacgct ggcatctgct gacggtacgc gtctgtggaa
      721 actgccggtg actcccttac tcaccttctc cgtgaatggt cagtccattc acgtcacccc
      781 tgaactggag taaatcatga gcgcagaaca ggacacagcc gcaaagtccg gcaaaaaact
      841 gtcagccctg ctggttctcg ggggcgtgat tatcatcggg ggcggatacc tgctgctcac
      901 ctggctttcc cccgaaaaca gcgcatcgct ctcgtcagtc aatatcaaca gcacgacaac
      961 aggcagcggg cggaccgtgg tggagtcaca gcactaccgg gatttactgc gtgctgacaa
     1021 tgagcgcggg gccgcagaag cgttacgtaa taaccagacg ttcatcgcca gtctgcccag
     1081 gggactggat actgatcacg gaaataatca aagaagggat actcagcctg ctgaccaggc
     1141 cgagggttct ggtcaaccat caacgccccc tcaggcagat caaacagccc gggaaaaaca
     1201 gcgggaagcg ttacaaaaac tgcttgcccg catcaacaca cagcatcctg acgcccgccc
     1261 cccggtagtg gcggctgcaa tgtggggtac cactggtgct cccgggataa gctcacacgc
     1321 ctcaccaccc ggtaccggca catcaccaca accacgtctg caggaggcca gtctggcaac
     1381 cccggcagct cgtacaggga tacagctgat accggcactg acccgcgtac ctgccttcat
     1441 tgatacggaa gtggactctg acaatgctgt ctcccgcgtg gtggcctccg tacctgccgg
     1501 cccatgggcc ggggccgtcc tctacagcca ggacgccaga ctcgccggca agggaatgga
     1561 tatccacttt gaccggatga tgtggcgggg catgtttctg aaagtcaatg catatgccct
     1621 gaatgaacat accggcatgt catccgtggc cagcgacgtg aaccaccgct ggtttaagca
     1681 tatcgttctg ccgtctgtgc tcggtggggt gggcagtgtc ggaagtcttt acaaagacgc
     1741 caacacacag gtcattcagg gaaactacgg cagcattacc ggcagggtgg gaatgcctga
     1801 tggcgaggcg ctggctggcg tcattgcagg cggcatggcc gaacggggca gtcagttgct
     1861 gacccgccag gcggagagcg aaccttaccg gcaggtgaca gtgtcacgcg gagaggttgt
     1921 ctccattctg tttgttgagc cggtcatgag caacgatatc agtcccccct cctccggcac
     1981 tgccccttct gtgccaccgc cctccggacg cccgctcacc caggaagaag cagagcgtca
     2041 ggcgtctgac aggatccagg cagctgtcat gcgtaaacag aacgaaatgc ggcaacgtta
     2101 cggaatacaa caggaaagtc atccatgaaa aacgaacatg aagatcccgg cagttttggc
     2161 gaaacaacga caccggcatc cggcaaatcc ttcctgaaac gccagtatat gggatacagc
     2221 ctgccagtct gggccggctg tctcctcatc atcctggccg cagtctggta caagtttctg
     2281 cgggcaccct cagagccccc tgcagatgca ggcttcgctg tcacaccgcc cgcagccgaa
     2341 agctgggcag agcccatgcc catgacggag accgggagcg gaaacaccat gtccgcgcct
     2401 cctgtcaccc cacaggcaga catgcagcag cttgcccgtg acctgaaagc agagctggac
     2461 aggcgggatg aggaaatccg acgtaccctg accatgctga aagagagcat aacaaccctt
     2521 ggcgacacca tacggaaaga tgaggcctgg gcacaggaaa cccgtcagtt gcttgatgaa
     2581 acccgtaagc aggtgcatgc cctgcagatg cagggaccac tgcaaatccc gaccgggaat
     2641 aacggccagt caacgaaaac cccgccttcc cgcctgcagg gcatgcaaat catgtcggtg
     2701 gaaaaaggaa tggcctggat ccgctggcag ggtagcgcct gggcagtacg ggaaggtgac
     2761 aggctgggga atatcaccat cacccggatt gaaccgtcca gccgctcagt catcacctct
     2821 gccggtacat tacgatgatg agaattgatg ccctgacagc catagagaac tttgccagcg
     2881 gtatattcag tgcaggcatg gagtttctct tcacctgggg agagctgctg gggattatcg
     2941 gcctgattgg tcatctgatg cgtgcccggg ctgaagggcg tcacagtatg ggaccgggaa
     3001 aatttatcgc cggtatcgtc atctgcggca tgctggtcgc cctgccatcc ttcatcaatg
     3061 ccggggggac ccagatggga ttcagggcag attccttcgg ccctatcgct tatgtacaac
     3121 caaccacctt tggtgccgct gccggtgccg ccaatgccat gctctcactg gtaaaactgg
     3181 ccggagtggg gtttgccatg aacggcattt ccatctggcg taaatccctg ctggacgggc
     3241 ataccgccct gagcagcagt gagtccatca gcaaaggaaa cgtcaagttt gttgccggcg
     3301 ttctgctggt ttttatcgat cgcgtcatcg atgcctctct ggcgagtctt ggtctcgcct
     3361 tctgatccac attcattttc agtaattcag tctgttagtt aaggagacta tatgtcacga
     3421 ttccgtgcca tggcggcgcg tctgtgcgct gcatttattt atgttcagtt atgcctggtg
     3481 gcctgccgtg accggctgat acgcctgtta cttctgcttc ccgtgatact gctgccccac
     3541 acagcgatgg caaacaccct attcgatatg atcaaaaccg ttggcgatgg tgcaaaaggc
     3601 tccagcagtt catttgtggc catcgctcag gttattggcc tggtgctctt tattgccggc
     3661 ctgcttggcc tcaaacaaat cggtaaaaaa cccggagcag ggctggcctg catactgtcc
     3721 attgtggtcg gtgcctgtct tgccgtcgta ccggaaatga tgagcagtgg acagaaaacg
     3781 ctcggtcttt ctccggtctc catcagttaa tccacaacgg caccggtttc ccggtgccgt
     3841 cttcacacaa tgaagaggac cagaccatgt cctgtgagct gccccctttc accctgctca
     3901 gaacagagca cggcaacgtg attgcccgcg acaaccggga taatcaggag tttgtgtttg
     3961 cagaatgtta ttccaccgcc atcgcagcac tgattctggc tgccgtcacc ctgtatacag
     4021 agagaaaaac gtatgaacaa aacaccgcca ctcatcgccc cggcccggga acgctggctt
     4081 ttcccgctcc cgcaaccgga cacggtattc agcggtgaat ccctgctgcc accacccgtg
     4141 ctctcaacac ccggacgctg tccctgctgt cgcaggaccg tcactcaccg cttcattctg
     4201 gaggattcat ggccattaca gcagatggct gacacctgcc gggatacggt cgtcctgctg
     4261 gaaaaaaacc tcacccgggt gatgcgcctg aaaaaacatc cggtaccgga aaacgctgac
     4321 gaaaaaaaga aacacacccg aaccctgcag gatgcggagc ggtccctggc acaggcccgc
     4381 ctgtcggcca ggcgactggc cctgcgtcat gtggaaaagt cacaaatcgt cacaacagat
     4441 gcactcagcg aaaatgaaag tgaactgctg cagcctgaag gcccgccctt tcatctctgt
     4501 gccttctgcc acgcctggca ctgcctgaac ggctacgctg ccgcacaggg ggtgatggtc
     4561 tggctccctg atttgcatcc ggcctcggta gttgctctca atgcacgggc actgaaggaa
     4621 attttcagcg atgagcgtaa gcgaatcaga cagggacggg ccgtactgaa tgccctggta
     4681 cagaaccgcc tggccgttga ggagaaattt cgcacctggc gtccggcgga ttttgctgat
     4741 gcactgcgcc gctggccgcc ggcacagcgt aaaacactgc gggagaaaat ggacggggtg
     4801 gcgctcattc tgatgccaga ttcgtttcct gacaaaaagt acgtcatgtg acacacaccg
     4861 ctgccggtga cgttctgccg gccccaactg acagaggaac aaaaaatgga taccctcact
     4921 gccggatatc agttttccgc agatgcattc cggcgcttta ttatccggac gctgactgac
     4981 gatattccgc ctgacggaga tgaaaaagag attgcccggt tcattctccg ccacccggca
     5041 tttaacggcg gctggctgct ctttctggct gcagaaattc atgaaagatc ccgtacccat
     5101 aacagcatca ccgtcacagt gagcgtcgat aactatctct catggatgga tattgccgtg
     5161 tccggtctcc cggaattacc ggatatgccc ttctgtgatt ttttcattcg ccgccagcag
     5221 tttatctatc cgggtgtacc cccgacaaaa tcaggagaag aatgatgaaa attcaccgca
     5281 tatccccgga gacgcttata acactcatcc tcgcacacct ggccggaaaa gctgacagta
     5341 ccgccaaaga agagcaccgg ctactccgtc ggttccttcg ggatgatgac ggcaggctgg
     5401 ccggcatact gctgaatatt gccggaatac ttcagttcaa cagggaatta agcgcccggc
     5461 ataattatcc ggccacaccc ctgactgaat tttcattaag gaaacgggga aaacaactac
     5521 atctctgtct ctgcagtctc cgtttctttt atatcccccc tgtatttata cgaaatgaaa
     5581 gaagaaaatg tattgttgta catattaata aaatcactta tccgccagta aattcactac
     5641 gctgacagcg tgtttccacc ttattacatt tttattatta actgtaatca cacagaaata
     5701 cgcacctgtc cctcaacaga ttatttattt tctctgctgt tttttcatgc cacatcccct
     5761 gctgtggcat caaaaaatat cagaccgtga tgattcatcc ttaccggagt gctccatgcc
     5821 atgaatatca accgcattat ttttgccatt gaagactgta tcagtaccct gtcacggtat
     5881 tccgtttccg cattcttccc cgattactgt gatctgcaca ccgtcatcgg tctggaccaa
     5941 caggaccgcg aacgccgccc ctggctgaaa gccccctaca ttgccaccac caaacagggg
     6001 tattaccttt ccgtctttga aatcagcggt gccctgaaag aaatggatga aaacccggac
     6061 ctgacagccc ccggcacact ggaatcactg attacagcgc tggcagagcg aatgaacact
     6121 gcctataaaa acatcggtca caaaatcagt tttgttttcg aacgcgatcc ggagcgtgga
     6181 aggaatgaaa tcgaagtcat gctcttaccc cagcgaaaat ccatcgcacg cacggggatc
     6241 ctgcttcagg acgttctgga tgaaaaagtc accacgctca ccccctggct tgtccgggaa
     6301 cgctgctggc tgaccgtctg gagctctgaa gaactgctga gccggaccga tcgcaaggac
     6361 caccagaccc gtatcaggaa actcgctgaa cacgctccgc cagcccgatt tgcgcaggat
     6421 ccatggcgct ggacactttc cgcactcaaa atacggcatg acgcctttct ggacacgctg
     6481 gagcaggccc tgacacatga ttctgatggt ctgctggttc gcctgatgga tattcatgaa
     6541 gtgggacggg aaatccgccg tcaactggag cgcaacagca cacctgcagt atggcagcct
     6601 catctgcctg aagatgcccg tcctgccggc tggcgacagg gcgaggatac ctctgtattc
     6661 cacgcaccgt cgctgaatct gcagttattc accacacagc ctgaaacaca cggcagcctg
     6721 ataaaggccg gtgacctctg gcacggtatg gtcgccatta ccctgccccc ccagaatctg
     6781 cggacattca accagctggt cagggatgtg ccccgcgcaa tcccctggcg tatccggatg
     6841 gatctgatgc ccggcggcat gaaggcgctt ggggtcaaga aaaccctgct gacctacagc
     6901 agctttattt cccctctccg gcccatgtat gactcagtga tgatgctgaa tgacatcaac
     6961 aaacatgatc cggtctgtat catgactatt gttgccacca catggggaaa cagccgggaa
     7021 gtctgtaccc gcaaccaggc gctcctgaaa tctgcgctgg aaggctgggg agtatgcggc
     7081 accaccacca ccttcggtga tccgagacgt gcctgggtga acacggttct ggcggcctcc
     7141 cacggttccg gtccagtccc cctttacccg cccctttcac acgccctttc cctgtttccc
     7201 ctgaacaggg ccggctctgt ctggcgggga cagggaaatc tgatgatgca caccgaagac
     7261 ggctctgcat gggaggtcgc gctggcatcg tcacaacaga acaagcacac ggaactgacg
     7321 cccggcgcac ccggtctggg caaatcggta ctcatcaacg cactgtcgga gatccagata
     7381 gcctcagccc agaagaacct gcctttcatt gcctatatcg acaagggatt cagtgcacag
     7441 ggactggtac agcttatccg tgacagcctg ccggagcaga gaaaggacga agccgtgggg
     7501 atcattctga gcaatgatcc ggaacacacc cgtaacctgt ttgatgtgat gtacggtgct
     7561 aaaaaacccg ccaccccgga aaagaacttc atggtatctg tactctgcgc actgtgcgtg
     7621 gataccggca ccggtcagcc ctgtaatccc ggtgacaccc ggcaaatcat aagcaacctg
     7681 gtggatctgg ccttcaggga gtatggcgaa aacaacccca gactgtaccg tgcaggaaca
     7741 gagccccttg tggatctcgc cctggaagag agtggcatcg cagaacaaca tgacgctgga
     7801 tggtggaatg ctgccacctg gtttgaaatc agggacatgc tgcatatggc tggcaatatc
     7861 cccgcagccc agcgggcaca ctaccaggcc atgcccctgc tggctgaaat gtccgccctg
     7921 ctgggacaac catccatccg ggatgtgttc ggcacagttc agcgcgataa ctcagcagaa
     7981 ttgttactgg actacatccg ccgtgccctc gatcagggac acagtgacta cccgatgatg
     8041 tccggctgta cccggtttat gctcagcccc gacacccgcg ttgtggccgt tgatctgaac
     8101 aatgtggccg gtgacaagac acctgccggg cgtctgcgca ctggcatcat gtacctgctg
     8161 gcaggacaaa tcgccggcgg ggattttgtc ctgccgcagt atcaggagga aatacggaaa
     8221 aatctcgacc cacgctacca tgaggtgatt ttccgccgca ttgaacagct tgatcaggag
     8281 gtcaagacca aagtctatga cgaactgcac aatgcaaaag ggatcaattt catctgggaa
     8341 gccctggaca cccaggagct ggagcagcgt aagttcggga tacgcacagt gctgagcacc
     8401 cagctattgc aggactatcc accggcagtt ctcaaatccg ccaacaccct ctggttgctg
     8461 cgttaccgcc ctgacgaaat tccgtttctg cgggataatt ttggtgtccc tgaagtcacc
     8521 ctgcggcgct tcctgaaaat gccggaaggg gctgcaccgg atggcagcgg tgtcccggta
     8581 ctggctgtgt tccgtgtaaa aaacggtacg ctggcccgga tcctgaaatt cactctcggt
     8641 ccactggagc tgtgggcact gaattcctca cccaaagaca gcgccctgag acgtgcgctg
     8701 acgcaggagc tcgggtcact gcgggcacgc cagatcctgg cagaaaactt tccccgtggt
     8761 tcagccacct cactgataga acaccgcgcc cgtacacatg acagtgaaaa cgtcatccat
     8821 gatctggctg cagaactcat ccgtaagcag ggatatcacc tctgatgtac agcatacaca
     8881 tcactccccg ctgccggcgt cctgcacggg gacaggtctt cctgaaaacg ctgcgcgtgg
     8941 acggtgacag tttcagcttc accctgccgt ccgcctgctt cacgctgcgt ccctccccca
     9001 atccccgggt cattttcgcc agtgacagac gggctgacgg ggaaatgagt ggcgttcgcc
     9061 tggctctgcc cggagagaca ggcacgctga ttacaacggc cacctggcag gtcaacgtgc
     9121 cggagcggcc tccttttgat atacagcatg tcattcactg gctcctgccc tcgcctctca
     9181 cggcgctgat gtccgacaac acctggctgt ggcaggatag ccccggcaca ccggcactga
     9241 cggcgggaga ctggccggta atggaaaccg ataccccgac gctgaaacgc gtgaagcgtc
     9301 gtaccttcac gctcatcgat aaccgcaacc gtcagggatg gatcacccgt cgcttccagt
     9361 gcttcccgct ggctccgctg ccggaatcac acagtcaccg cgtaccagac atcctgtatc
     9421 ccctgaaaac ggagaaaaaa gaatgcaaag acaaccagca accacactgc ttgcactcct
     9481 gataggctgt gcggttatcc cgtccaccca cgcctattct gtgaccgtag tggccagcca
     9541 gcctgtcacc acacaaatca tgccggagct ggccgccatt cagggcgtga tggcagagat
     9601 ggtccgtatg cagacagcca ccggcacagc catcaaccag agttcagaga aactggcctc
     9661 tgtcattgcg caggatgggc aggccacccg tcagcagatg atattcagta atgaaacccg
     9721 ccggcttgag gaagcccgga agagcttcag cgtgccggac tccatctgca gtgaatccgc
     9781 ctccggagtt gcagctgaaa gtcgccgtgc agcagcgtct gcggctgcac gtctgagtca
     9841 gggaggaggc gtcagcagta aaccaatccg tgaacgtctc tcccgggcag cagactcacc
     9901 aatccgcgag gcatatgaca gtgcaggcat tcatgccggt tactgcacgg aagctgaata
     9961 tgcccgcttt ggcggaaccg atgtctgccc tgccgtgggc gagctgccgg gcggagacag
    10021 ccaggtgcga tcgctctatc agggtgcagg cacagcagat acccctgccg ccctcacctg
    10081 ggatcagaaa cagattgatg ctgccacggc ttacatgaaa aacaccgcca gaccgtcagc
    10141 gggtcgtgcc ccggggaaag gtgaagtggg aacacagacc gggcgcacgt atgtgggcct
    10201 gcagaatgaa tacaacggca tcatcgatgc cgcttctcat ccgcaactgt cactgatagc
    10261 tgacagtacc cctaatgagg ccacccgtgg agcgctgact gaagccctgc agtcaccatc
    10321 cgctgcggca tattttgacc ggaccgcatc atcagaggcc cgcacccgtg gtcacatgag
    10381 ccagcgggag tttgaggcct ttgaagctgg cagacgctat gcaaacaccg actggcagca
    10441 ggatctgcag ggcatggaag gcgataacct tctccgtgaa ctgctgcgca ctaccgccct
    10501 gctgaactgg cagatgaacg acctgaaaga gcagatacgc cagggtaatg ttatcgccgg
    10561 gcagcagctg gcactggcgg cacgacagta ttacggacag cgtctcggtg aactgtcaca
    10621 ggccatgagc caggggagcg taagatgaaa gacgacaaca gcaccgacaa aaaaaaaccg
    10681 gccccccgtc tccgccgcat cggtgacatc gggataaacc tgttcgcacc actggcagaa
    10741 acccggtgga tgctgcgcag ctgccgccat atgctgggcc agcataaaac gcgcctgaag
    10801 cgcctcatgc ccgaccggca accggagagt ccggtctgta tgacctggga agaagccgtc
    10861 agggccagcg gccttacggt caacgaactg gatcggcgtc tgcgacgtcg caggatgttc
    10921 tggcggacag gattctggct gatgatgata ccggtacttc tgctgtgcgg catggccctt
    10981 gctgccacag cgcttccggc catcaccctg atacgtatac tggcctgcct gttactgatg
    11041 agtgccattg cagcactgtg tgcctcaaaa accctgattg tgtcataccg cctctggcaa
    11101 ctgaatgaac ggcgggtcag cgaggacgaa cagggcacct tccccgatta cctgcaggcc
    11161 cggcacggct ggaaacaggc cacatggctg gccattaccc accggcagta ctgaccgtcc
    11221 ggtctgcaat tccttttcct cattccggag gctgatgccc tgcatccctt cggttccgga
    11281 gttatcgtga aaatcatacc cagagcactg agtgcgggtc tggcgctggc tgccgtacct
    11341 gcacaggcat tcgtcaccta tcaggacatt gtgagcgccg ccaccaatcc ggatgacctg
    11401 tcgcgccagg cactggtcac catttttggc gacgtggtca caaatcccct gacagccagc
    11461 agcaacacca ttatcggtaa cctgttcatg gtgtttaacg caattatcgc cattatgggg
    11521 atagtctggt tcctgtttat tggaatccgc catgttgtcc gcaccggcca ccagggcaat
    11581 gtctttaaca acggacggga cgtcgtcgga atgctcagta ttgtgagcgg ttttatgatg
    11641 attgtgccca ccgcttccgg ctggagcctg gcacaactgg tcatgctctg gggcgcatcc
    11701 atcatgggca ttggttccgc caatatcatg gttcagactg ctgcagaaaa catcgctgaa
    11761 ggctattcaa tgaccgtaca gccagttcat gtctccaccc gcacggcagc acggggaata
    11821 ctggagatgg cgctgtgcaa acacgccatg aatgcaggcc tcgatgactt taaccgtact
    11881 gcccgttcat caacgccccc catgagcgaa tcctcacgga cagagaacgg caggtatacc
    11941 gtcaccgttt caaatggcag tgccacctgc gggagtgtga gtctcaccgt gaaagggaat
    12001 ggcagcaaca accagtccgc actggactat tttttcaatc ccttcattga aacggagtat
    12061 cagggtgtcc tgacggcaca acgctccgcc atggatacca tgctccgtga cctggacagc
    12121 gcagcacagg aatttgtgtc tgcgtaccgg acccgacgcg acacgggtaa cggcaacctg
    12181 ccggatattg aaagccgtat tcagaaggct gccaaccggt atgaagaaac ggtgcagaag
    12241 gcgttgccaa cagataacgc agaaactgag cgcagggagc agctgaaata ctacctgacc
    12301 acctacggct gggttgccct gggggcctgg taccagacct ttgccaccgc caaccagcga
    12361 ctggcagaac tggccgaccg tgcccccact gtgagtggag tttcatcgca gggcgaagcc
    12421 ggagatactg aattcctgaa ggaagtaaaa ggagcttacc aggcacagtt gcaaaactca
    12481 ccatatacac ctgcactggg aacaaccgct tcatcaactg aatacagaac agcagaagct
    12541 gctgaaccta aaagcataat catgaaagta tggtcgggtg taaattttgc agacaaaatt
    12601 gcaacatcct tatctggaac aggaaccacc tcagaccaac taaatcctct gataaaaatg
    12661 aaaaatatcg gtgattacac tatggtcaca acagaaacca tctgggcggg ttataccacc
    12721 gtcagggtac tttccactgc cggcagcaac tctatttttg gtcgactggt caatgccgtt
    12781 agcggcttat ctgatgccgc aaaggctttt ctggaagcag ctgcaccacc gctctacttc
    12841 ctgctgtttt tgctgttctg tgccggtttt tcgttatcca tttacctgcc gtttattccg
    12901 tttatcttct ggatgaccgg catcggtaac tggatagtca gtgtactgat tggatgcgcc
    12961 gccggtcccc tgtgggctgc cacccatctg gggacaaacc aggaccgggg aagccgggct
    13021 gcttacggtt acatctacct gatagacagc atgatcagac cactgttgat ggtgttcggg
    13081 ttcttttttg catcggtcgc catcgtggct ggcggcacca tactgaatgc gttgtttggc
    13141 gcggctctgg ttaatgtgca ggctgactcc cttacagggt tattcagtat cgcgggattt
    13201 ttgatgattt atgcccgtgc ctgtaccacg ctggttgcgg ccgtttttgc gcttcaggct
    13261 tatctgcctg accatgtaat taattttctg ggcggacgtg acggcgttaa tatgctgggg
    13321 aatatggttt catccgtcaa agatattatt gccggtggca acaccaatat acgcagaaca
    13381 cctggcgttc gtgaagaaaa actgaaaaac attaatggag atgataaaga cggaataaaa
    13441 caataaagta cagacatcaa aaaaaacagt attgccacat tcacctgaag agattaaaat
    13501 acattcaggt gaatatggag gtttattatg aacacaacaa aaagcagata tccttcctgg
    13561 tgggcatatt atcaactagc ccgcaatact tactttatat ttggaattcc atgcttatta
    13621 ttctatgctt ttttcggaac actaattgtt catgactcaa gcttatctca aacaagagat
    13681 tttatttttg catatggatg ctgggcatta ataattctcc cattactctg gctatatctg
    13741 aaaaccagag cgaagaaaaa gaaagtccag caggttgttc agcagataaa agaaagcagt
    13801 ccctttgcac cgaccaacga ttacgagcaa ctgtcattct cgaagagttg ttatttcggg
    13861 atcgacatta aaaacggcac catgctgtat gtgcgcattt acccaaacaa tgtcatggat
    13921 gtgattggcc tggatattca caacttcacc cgaacagttg ctgaagacgg caagctggaa
    13981 atccacacca cctatgtgag tctgccgatg atccctcttg aaatctccgg catctcagca
    14041 aggacgctgg ccaacaccat acacaccatg gctgcacgtg gatatgagta caaagagcgt
    14101 ttcccgcaga tgatccggta tcgggttaaa gagtgggaaa aagttgccgg tgttcctgtt
    14161 gcagaagtat tttgaggtag ctatcaactg ttagacgata atccatcagc cggttttctc
    14221 tgaaccggct ttttcttttc aggcagaatg cctgtctgcc gtccctgtat cagactgtaa
    14281 ttagcacaat acggcaattt gccgttcagt atattcagga agtacaaatc atgccatctg
    14341 caacacaaac tgcccgactg gaagcaagga tcagcacaga actgcatgct atgctgaaac
    14401 gcgctgcaga aatccaggta aaaaagggcg cattgaggtt tgctttcgca acaatccggc
    14461 ggcagatttc ctgccgccgt gttgcccatt aacgtttaac ttcgtaggct aacgttgcca
    14521 caagcactgt gtttccctgc tcgacacgga atccgcaaag cgaatccctc accatccaga
    14581 caaaacacag tatcgtcaca cagacgacaa ttaacattgt taaaaacgtt cgttgtggca
    14641 tcgcagcccc tccattgcct ttcggccgtc atcgggctac tataatgttg tctaggcatt
    14701 acagtgggcc ctgggttgat tgataaaaaa atcgcctggg gctttctgct gcatgccgtc
    14761 cacaaaacgc tttcagcata cagcctcaag cgcccgccgc tattctaatc gattttcagt
    14821 ctgccacaat cgttttccac ctaatgacat gttatgcaca acagtatttt tacgggaaac
    14881 tacacggcaa ggattcccgg aacacgttca ttatttgatg gttacagaca tcatgttaaa
    14941 tccggaagta ataatgacct gaaaggaaat actatgagta acgaatatca aagcaccagt
    15001 ataaaccctg atgtttttga acaatttctt accctcctga atacacactg taaatcatca
    15061 gaagctgcac ttgccatgct gagtacactc tgctacgaac tgatgaggag gagtgggatc
    15121 cattcactgg acagcctgat gctcaacgga gaaggtatat tgatacaatt acagggggct
    15181 gatattcgcg aaaaaacagg tcagcatatt cactgatcca gggatgtgct gaaaacagtg
    15241 tcagcctgaa ataaacgttc aatgcctcat acacatgagg cattgagcac ggtcatcaat
    15301 aatctttatt atttttctga ttcagcattt ccggtcagta tcggctcttc tggcaaagac
    15361 attaccggaa cgtcacgttc accattgaat acagaaacac tttcagcgaa atcattctgt
    15421 aacaaagaaa aacttaacgg cagatttttg gtttgtatcc ctgtgatcca ttgaaaaatc
    15481 tcgggaaaac gacgtgcact gatatgcaca tgattgaggt ggtgctcagt aatatcactg
    15541 gccatcatca tgagaggtac atcgtatccc gaacgcaaac ctggcgcatg atgcacaggg
    15601 gtgctgtctg aactgacgga ttgcccatga tcagaaaaat acagcatggc aaaatcatca
    15661 ccatgctctg tcgtcatgtt ataaattgcc tgaagaaacc gatccagttt aagcagtgtt
    15721 gataaataac aggatacttt atgtccgaat aattccctga atggctgtgc atcattaaaa
    15781 agtcggtcac agggattagt gtgagacccc agcatatgaa taaaaatcac ttttggagaa
    15841 gacgagttaa cagcttccct gacatactga agcatatcct catcatcaat gcgaagggaa
    15901 gaagagctcc cttccttaag aaaatgtttt ttatccgcat tggcggcgat gacagaaatc
    15961 agcgtatcat gttgccctaa agagccctga tttgatatcc accacgtctc atacccggct
    16021 tttctggcaa gcgtaacaat attattaccc ggattacctg tttcagtgta atcaagcgtc
    16081 agtgttctgc tgagtgaacc tatcgtattc ggcgctgctg agaaataccc attaataaat
    16141 atacccggag cagaattcag ccatggtgtt gtcggcaaag gatacccata aacagaaaga
    16201 taatcccgtc tgacactttc accaattacg ataattatgg ttttatattt cttctccaca
    16261 ctcacaatat cccatgagtc tttctgattg tttttcagcg attcatatcg catcatttct
    16321 tctctggttt cagctatccc tgtcattaca ttcgtaacca tacgttttgg ccatgcattt
    16381 actgacaata cgataaagaa caacagaaag ataaatcccc ggtggctttt atcacgctta
    16441 cgccagaacc agaatgtaaa agcagcaatg accagcgaaa caataaaata aagaacaggt
    16501 atgcctgtga taaactcacc tgcctcattt atatttgtct gcattacaga cgcaataaaa
    16561 ctgttgttta ttttcccgta aatcatccca accggagcat agaatgcact taacaaaaga
    16621 aagatacaga cacacgttgc tgaaataaca ttccgccgcc ccaacaacat catcaggaac
    16681 aatataagca cattccccag ggcgtttttc actgtatatc cgcagcacca tgtaacaagt
    16741 acagaaaaca acagataaat cagtgactct ttacaccaca acataaaata aaactgtttt
    16801 gctctctcaa aagcatattt cgacttttct ttaatacaag acacgccagt cattttcctg
    16861 tacaaacaca gacgatcatt tcaacgcatg gatatttata tgaaacagtt cacaaaaata
    16921 ccctaccctg tccagcaggg tttacatatt atcaggttct gtgtttacag caactgctcc
    16981 ccgaaccaga ttattcgcat aacccgatga aggcctcata accatcaaac actcagaata
    17041 catttagtta acaaaaatat gtcttgccct cccggaaagg caaacatacg aaagagaaag
    17101 catggctata ataatatcaa tcaaaatatg ctcaatcttt ccggttgcca tacactcagc
    17161 accgacaaca taaagacaa
//
LOCUS       DAZUCC010000103        17121 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZUCC010000103 DAZUCC010000000
VERSION     DAZUCC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.103"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            539..1627
                     /gene="pdeL"
                     /locus_tag="ACHY09_002854"
     CDS             539..1627
                     /gene="pdeL"
                     /locus_tag="ACHY09_002854"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFS3885807.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1669..2601)
                     /gene="yahB"
                     /locus_tag="ACHY09_002855"
     CDS             complement(1669..2601)
                     /gene="yahB"
                     /locus_tag="ACHY09_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3885808.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(2693..3190)
                     /gene="yahC"
                     /locus_tag="ACHY09_002856"
     CDS             complement(2693..3190)
                     /gene="yahC"
                     /locus_tag="ACHY09_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFS3885809.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYFGMVWAFFAALAAGWLASVSGISGFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3448..4053
                     /gene="yahD"
                     /locus_tag="ACHY09_002857"
     CDS             3448..4053
                     /gene="yahD"
                     /locus_tag="ACHY09_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFS3885810.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLNIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            4093..4956
                     /gene="yahE"
                     /locus_tag="ACHY09_002858"
     CDS             4093..4956
                     /gene="yahE"
                     /locus_tag="ACHY09_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFS3885811.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYLGLQCGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4946..6493
                     /gene="fdrA"
                     /locus_tag="ACHY09_002859"
     CDS             4946..6493
                     /gene="fdrA"
                     /locus_tag="ACHY09_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFS3885812.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLRNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGMKQEHLDLHTLNQPLIADVRA
                     RMQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSVKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6493..7911
                     /gene="yahG"
                     /locus_tag="ACHY09_002860"
     CDS             6493..7911
                     /gene="yahG"
                     /locus_tag="ACHY09_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFS3885813.1"
                     /translation="MSPSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPTAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7937..8191
                     /locus_tag="ACHY09_002861"
                     /pseudo
     CDS             7937..8191
                     /locus_tag="ACHY09_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8241..9191
                     /gene="yahI"
                     /locus_tag="ACHY09_002862"
     CDS             8241..9191
                     /gene="yahI"
                     /locus_tag="ACHY09_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFS3885814.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGVFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPQRIVEAPAIKALIQQGFVVIGAGGGGIPVVCSEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            9201..10583
                     /gene="yahJ"
                     /locus_tag="ACHY09_002863"
     CDS             9201..10583
                     /gene="yahJ"
                     /locus_tag="ACHY09_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS3885815.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTIHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVQIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGNVAG"
     gene            10879..11289
                     /locus_tag="ACHY09_002864"
     CDS             10879..11289
                     /locus_tag="ACHY09_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS3885816.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPLIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            11543..12526
                     /locus_tag="ACHY09_002865"
     CDS             11543..12526
                     /locus_tag="ACHY09_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3885817.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            12575..14059
                     /locus_tag="ACHY09_002866"
     CDS             12575..14059
                     /locus_tag="ACHY09_002866"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFS3885818.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            14052..15023
                     /locus_tag="ACHY09_002867"
     CDS             14052..15023
                     /locus_tag="ACHY09_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3885819.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKMGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            15020..15976
                     /locus_tag="ACHY09_002868"
     CDS             15020..15976
                     /locus_tag="ACHY09_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3885820.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            16063..17112
                     /gene="yahK"
                     /locus_tag="ACHY09_002869"
     CDS             16063..17112
                     /gene="yahK"
                     /locus_tag="ACHY09_002869"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFS3885821.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEHLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSELKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         4228 a         4029 c         4636 g         4228 t
ORIGIN      
        1 gtttttattg tcattgttat aatgttggtc atctattgct gaatggattc agtcttaaga
       61 ggcaggttgt taagggatag gcatagagta atgacgtgta tgcataacca acatctttac
      121 tcattatgtc attgaatgtt gacgctatgt gtttatgagg ttgaggtatt ttcagttgat
      181 ctggattgtt aaattcatat aatgcgcctt tactcatgaa tgggcgtcaa tacgtagtgg
      241 gaaattataa atatcgaaat agtccaacta cttctttatt accaaaaatg agtatctgaa
      301 ttttaatatt gcattcttgc gtgattatct cctgagtttt gacttgtgat taccttttta
      361 aggtatttag cgtaactgtt tctgagcgag catcagaggt aaagataatc ttcttgatag
      421 tgatgtggga tgttatacgt atggcatcgc taatgtttat ggttacccct tatgtgtgct
      481 caggaatcca caggtaatca ctcatactga acagcgataa aagataaaag gtgtgttcat
      541 gaattcatgt gattttcgtg tttttctgca agagttcggt acaacggttc atttgtcatt
      601 gcctggtagc gtatccgaga aagaacgact gctactcaag ctgctgatgc agggaatgtc
      661 tgtaacagaa atatcacagt acagaaatcg tagtgcaaag accatttcac atcaaaagaa
      721 acagctcttt gagaaactgg ggattcagag cgatattact ttctggcggg atattttctt
      781 tcagtacaat ccggagatca tatccgccac gggtaataat agtcacaaat atattaatga
      841 taatcactat caccatatcg tcacgcctga agccatcagt ctggcgttgg aaaaccatga
      901 atttaaaccg tggatccaac cggttttctg cgcgcagact ggggtactga cgggctgtga
      961 ggtgcttgtc cgctgggaac atccacaaac gggaattatc ccaccggatc agtttattcc
     1021 tctggcggag tcatccggtc ttattgtcat aatgacccgc cagttgatga aacagactgc
     1081 ggatattctg atgccggtaa aacatttgct gccggacaat ttccatattg gcatcaacgt
     1141 ctcggcgggt tgttttttgg ctgcgggatt tgaaaaagag tgtctgaacc tggttaagaa
     1201 attaggtaac gataaaatca aactggttct cgagctaacg gaacgtaacc ctattccggt
     1261 aacgccagaa gccagagcga tatttgacag ccttcatcag cacaacatta cctttgcgct
     1321 ggatgacttt ggtacgggtt atgcgaccta tcgttacttg caggcgttcc cggtcgattt
     1381 tattaagatc gataagtcat ttgtgcaaat ggcgagcgtg gacgaaatat ccggtcatat
     1441 tgtggacaat attgtcgaac tggcgcgtaa gcctggtctg agtatcgtgg cggaaggggt
     1501 agaaacccag gagcaggcgg atttaatgat cggcaaaggc gttcactttt tgcagggcta
     1561 tttgtactct ccgccagtac cgggtaataa atttatctct gaatgggtaa tgaaagcagg
     1621 tggttgatgt aaaccgctat tcacagcgca tcgggaggtt ggcagcgatt aattctcccg
     1681 atgcagtaaa tcctgataga tccccgttaa tatgccattt ggcgaaaatt gttttttaat
     1741 ccactgcgtc acctgacccg tagcagaatg ctgagttgcg agtaacatgc gtgaatcctg
     1801 gcgcggatta tggatttgtc gggtaaccag cagggattgc gccatcgcct cacggaccat
     1861 gtaatccggc aaaaaaccaa ttccttcacc taaaatctgg cactgacatt tggtgttgaa
     1921 atctggcacc agaatcgact cctgcccatg cagcaaccag cccacttttt tattaatcgt
     1981 atgcgcggtg tcctccacca tgatattggg gtaaagacgc agctggcttt cggcgatcgg
     2041 ctccggcaca aaggctaatg ggtgatccgg ggcgatagca aaagcccagc gaattgcgcc
     2101 aatctccgtg taatcaatgc cgccgccgtc cagcagcgta tcgggtgcgc cgatagcaat
     2161 attcgcctga ttattaataa tcgcatccca gacgccgtta tacacttcgg tcgtcacggt
     2221 aatctggcag gttggaaact gctttttcag tacctgtaat aagcgtgcgg tgtgtttggg
     2281 cgtataaagc agttggttaa tacaaatgcg cacccgtgct tctataccct gcgaaatagt
     2341 atcgatgctg cgtttgatgg cgtgaaaatc attcagcagg tcggtagctt tgcggaaaaa
     2401 ataacgcccg gactccgtta actcaatgct tcgggtactg cgagtgaaca gcaccacatc
     2461 cagccctgtt tccattcgct tgatggtgta gctaatggcg gaagtggtta aacccaactc
     2521 ttctgcggct ttactgaaac tgccaaaacg cgcagcggta gtaaatgcca acagattctc
     2581 ttcggtaaaa attgagttca ttaaaccatt cccgccatta tcgaatatga atttctccag
     2641 ctatgactgt agaggtatcg gttaaagata gataatcatt tttgaataac ttttaatacc
     2701 cgtcgcgttt agtcgtcgct tcgcttaatt tttgtccgcc atattctgaa attatcccca
     2761 cgcagttacc cgccagaaaa cccagtaatg tcaccgtcca gttcatcccg ctggcaaaaa
     2821 atagcgtcat gccgagaaag cctccgggaa taaaagacag taaaccgaaa cgtccttgcc
     2881 agaccaccac cgccgagaag ggtacagtca taatcacgct cgcccagaac ccggaaatcc
     2941 cacttacaga ggccagccag ccggcagcca gcgcggcgaa aaaggcccac accatcccga
     3001 aataattaac cgccagactt ctgataaacc ccgatttacc gccgcctgcg gcataaaaac
     3061 tgcaaaaggc gacgaagcca atggttccta ataattccca tccctgagag agcccgacat
     3121 gtgaagacac taactgccaa agccctgcac aaataccgac ggtgacaccc gttgccgtga
     3181 gtccgttcat cttgttcccc tctggttaga catggacgaa agctactctt acatgtgcag
     3241 actatagaga aataaaacct gattgagtga aagggtatcg ggtcaaagaa acaaatattg
     3301 cactaccgca cactgcgaaa agattgttga acatcattca acaaaaaggc gaaatacggc
     3361 gaattaaatt taagcgggtt cttttatcag attatcgttg ctattctcaa ttcatcgaaa
     3421 acagccacaa aaaccagggg aatgtgaatg actataaaaa atctacctgc cgactattta
     3481 ttggctgcgc aacagggtga tatcgataaa gtaaaaacct gccttgcgct gggtgtcgat
     3541 attaatacct gcgatcgtca ggggaaaacg gcaattacgc tggcaagttt atatcagcaa
     3601 tatgcttgcg ttcaggcatt aattgatgcc ggagcggata ttaataagca agatcatacc
     3661 tgtttaaatc cttttttaat tagctgtctg aacgatgatt taacgctact acgaattatt
     3721 ttaccggcta aacccgatct taattgcgtc acccgttttg gcggtgtcgg cctgacgcct
     3781 gcctgtgaaa aaggccattt aaatattgta aaagagcttt tggcgcatac ggagattaac
     3841 gttaaccaga ccaaccatgt tggctggacg ccgctactgg aagcgattgt gcttaatgat
     3901 ggcgggatta aacagcaggc gattgtccag ttattgctgg aacacggtgc cagcccgcac
     3961 atgacggata aatatggcaa aacgccgctg gaactggcgc gggaacgagg ctttgaagag
     4021 attgcgcagt tactgattgc cgcaggtgca taaaccggga ggcttgctat caacacacca
     4081 gaaagacggt gtgtgtgggc gctaactgcg gatgctgatt ttctggcgca gcgggggcaa
     4141 ggacaggttg aacaggtctt tgccagagcg gtaaatatcg cacttccggc ccgccagcag
     4201 ttgctgacgc tgctttgtga agagtacgac aatgcgccaa acagttgtcg gttggcactc
     4261 actcactttg atggtctgtt ccggcatggt gataaggttc agtttgacga tcaaggcatt
     4321 acggttggtc aacatcttca tatagagatg agtcgttgtc ggcgttggct gtccccaacc
     4381 ttgcaaatgg ccgctgtgaa ttttcacctt atcgcctggc aacagtggca tgacattatt
     4441 catcagcacc tgggggaaaa tgaaaccctg tttaattatt gcggcgataa tccgttttat
     4501 caggcgttaa ataaagaatt acatattaaa cgacgggcag ttattcaggc cgtaaacgat
     4561 aaacaaaata tcgcctcagc agtcgccagt atgatgggct tagggattgg ccttacgcca
     4621 tcagccgacg attatttaac aggtctggcg cttattttat ttatttccgg acatccggcg
     4681 gaaaaataca aagaggaatt ttatctcggt ctgcaatgcg gcagaaataa taccacgtta
     4741 ttaagtgcca taacgctgga agccgcatta caacaacgct gccgggaaaa tattcatcgt
     4801 tttattcata acattattta tgacatccct gggaacgcaa ctcaggcaat agaaaaaatt
     4861 aaacatattg gctccagttc cggctgcgac atgctgtatg gcatggccga tggttgtgcg
     4921 ctgagccaaa cctacggagg gaattatgtc agttaaaata gtcattaaac cgaataccta
     4981 ttttgattct gtctcgctga tgtctatctc cacgcgtgca aataaactcg acggcgtcga
     5041 gcaggcattt gtggcgatgg cgaccgaaat gaacaaaggc gtgctgagga atttaggact
     5101 gctgacgccg gaactggagc aggcgaaaaa cggtgacctg atgattgtca tcaatggtaa
     5161 atcgggtgcg gacaacgagc agttgctggt ggagattgaa gaactgttca acagcaaagc
     5221 gcaaagtggc tcgcacgagg cgcgttacgc cactattgcc agcgccaaaa agcatatccc
     5281 ggaaagtaac ctggcggtga tttcggtcaa cggtctgttt gccgctcgcg aagcgcgtca
     5341 ggcgctgcaa aacgatctta acgtgatgct gttttccgat aacgtctcag ttgaagatga
     5401 actggcgctc aaacagttgg cccacgaaaa agggctgctg atgatggggc cagactgcgg
     5461 tacggcgatt atcaacggcg cggcgctctg ctttggtaac gccgtgcgtc gcggcaacat
     5521 cggcattgtt ggcgcatccg gcaccggtag ccaggaactg agcgtccgca ttcatgaatt
     5581 tggcggcggc gtttcgcaac tgatcggcac cggcgggcgg gacctgagcg agaaaatcgg
     5641 cggcctgatg atgctcgacg ctatcgggat gctggaaaac gatccgcaaa ctgaaatcat
     5701 tgtgcttatc tccaaaccgc cagcgcctgc ggtggcccgc aaagtgctgg aacgcgcacg
     5761 cgcctgccgt aagccagtgg tagtctgctt cctcggtcgt ggcgaaacgc cagtggatga
     5821 gcaggggcta cagtttgctc gcggcaccaa agaagcggca ctaaaagcgg tgatgctctc
     5881 cggtatgaaa caagaacatc tcgacttgca taccctgaac cagccgttga ttgcggatgt
     5941 gcgtgcgcgt atgcaaccgc agcagaaata cattcgtggc ctgttctgcg gcggcacgct
     6001 gtgtgacgaa accatgtttg cggtgatgga aaaacatggc gatgtctaca gcaacattca
     6061 gcccgatcct gaattccgcc tgcaagatat caaccgcagc gtcaaacata ccttcctcga
     6121 ctttggcgat gacgatttta ccaacggcaa gccgcatcca atgattgacc cgaccaaccg
     6181 catcagccgg ttgatcgaag aagcgcgcga tccagaagtg gcggtgatcg tgatggattt
     6241 tgtgctcggg tttggatcgc atgaagatcc ggtcggttcc accatcgagg cgatcaaaga
     6301 ggcgaaagcg atcgccgctg ccgaagggcg cgagttgatc attctcgcct atgtgctggg
     6361 taccgatctc gatacgccat cgttagaaca acaaagccag atgttgcttg atgccggagt
     6421 gattctggcc agcagcagca ccaataccgg attgctggcg cgtgaattta tctgcaaagg
     6481 ggaggaagcc tgatgagccc gtcactgttt agccaaccgt tgaacgttat taacgtcggt
     6541 atcgccatgt ttagcgatga tctgaaaaag cagcatgtag aagtgaccca actcgactgg
     6601 acgccgccgg ggcagggcaa tatgcaggtg gtgcaggcgc tggacaacat tgccgattcc
     6661 ccgctggcgg acaaaatcgc cgccgctaac cagcaggcgc tggagcgcat tatccagtcg
     6721 catccggtgc tgattggttt tgatcaggcg attaacgtgg tgccgggtat gacgccgaaa
     6781 accattcttc acgccgggcc gccgatcacc tgggaaaaaa tgtgtggcgc gatgaaaggc
     6841 gcggtcaccg gagcgctggt gttcgaaggg ctggcgaaag atctcgatga ggcggctgaa
     6901 ctggctgcct ccggggagat caccttctcg ccgtgccacg agcacgactg cgtgggttcg
     6961 atggcgggtg ttacctcggc ctcgatgttt atgcacatcg taaaaaacaa aacctacggc
     7021 aacatcgctt acaccaatat gagcgagcag atggcgaaga ttttgcgtat gggcgctaac
     7081 gaccagagcg tgattgaccg cctcaactgg atgcgcgatg tgcaggggcc catactgcgc
     7141 gacgcgatga aaattatcgg cgaaatcgat ctgcgcttaa tgctggcgca agcgctgcat
     7201 atgggcgatg agtgccataa ccgcaataac gccgggacga cgctgctgat tcaggcgctg
     7261 acgccaggga ttattcaggc gggctattcc gtcgaacaac agcgggaagt gtttgagttt
     7321 gtcgccagca gcgactactt ctccggcccg acgtggatgg cgatgtgtaa agcggcgatg
     7381 gatgcggcgc acggcattga atacagcact gtggtcacca ccatggcgcg taacggcgtc
     7441 gagttcggcc tgcgggtcag cggcctgccg gggcaatggt ttaccggccc ggcgcagcag
     7501 gtgatcggcc cgatgtttgc cggttataag ccggaagatt cggggctgga tatcggcgac
     7561 agcgccatca ctgaaaccta cggcattggc ggatttgcca tggcgaccgc gcctgcaatc
     7621 gttgcgctgg tgggcggcac ggtggaagag gctatcgatt tctcccgcca gatgcgtgaa
     7681 atcactctcg gtgaaaaccc caacgtcacc attccgctgc tcggttttat gggggtgccg
     7741 acggcaatcg acatcacccg cgtgggcagt agcggcattc tgccggtgat caacaccgcc
     7801 atcgcccata aagatgcggg cgtcggcatg attggcgcgg gcattgtgca tccaccattc
     7861 gcctgcttcg agaaagccat tcttggctgg tgcgaacgtt acggcgtctg agttacatgt
     7921 ttatgccgga tgcgacatga atgtcttatc cggcctacga atggcgcgga aatctgtagg
     7981 cctgataagc gtggcgcttc aggcattggc caccacagcc ggatgcgacg tgaacgcctt
     8041 atccggccta cgaatggcac ggaaatctgt aggcctgata agcgtagcgc atcaggcatt
     8101 ggtcaccaca gccggatgcg gcgtgaacgc cttatccggc ctacgagtgg cgcaagaatc
     8161 tgtaggcctg ataagcgtag cgcatcaggc agtctggcgt tggtcataac cccatcaccc
     8221 tctgtagcgg acataaaaat atgaaagaac ttgtggtcgt tgccattggt ggcaacagca
     8281 ttatcaaaga taacgccagc cagtcgattg agcatcaggc ggaggcggtg aaagccgtcg
     8341 ccgatacggt gctggaaatg ctggcttccg attacgacat tgtgctgacc cacggtaacg
     8401 ggccgcaggt cgggctggat ttacgccgcg cggagattgc tcacgagcgc gaagggctgc
     8461 ccttaacgcc gctggcaaac tgtgtggcgg atacgcaagg tggcattggc tatctgatcc
     8521 agcaggcgct gaacaaccgg ctggcccgcc acggcgagaa aaaagccgtc accgtggtga
     8581 ctcaggtgga agtggataaa aacgatccgg gatttgccca tcccaccaag cctatcggcg
     8641 tattcttcag tgaaagccag cgtgacgaat tacaaaaggc aaaccctgac tggcgttttg
     8701 ttgaagatgc cggacggggc tatcgccgcg tggtcgcctc gccggaaccg caacgtattg
     8761 tagaagcacc tgccattaag gccctgatcc aacaaggctt tgtggtgatt ggcgcgggcg
     8821 gcggcggaat tcctgtggtg tgcagtgaag cgggagatta ccaaagcgtg gacgcggtta
     8881 tcgacaaaga tctctctacc gcgctattgg cccgtgaaat tcacgccgac attctggtaa
     8941 ttaccaccgg cgtcgaaaaa gtgtgtattc actttggcaa accgcagcag caggcgctgg
     9001 atcgggtgga tattgccacc atgacccgct atatgcagga agggcatttc ccacccggca
     9061 gcatgttgcc aaaaatcatc gccagcctga cgttcctgga gcagggcggt aaagaagtga
     9121 ttatcaccac gccggaatgc ctgcctgcgg cgctgcgtgg tgaaacgggc acccatatta
     9181 ttaaaacgta aggacgtaag atgaaagaaa acaatagccg ccgtgagttt ctgagccaga
     9241 gcggtaagat ggtcaccgcc gccgcgctgt ttggtacctc tgtgccgctc gcccatgcgg
     9301 cagtctctgg cacaatccat tgcgacgcga ataacaccat gaaaatcact gacccgcact
     9361 actatctcga caacgtgttg ctggaaaccg gttttgacta cgaaaatggc gtggcggtgc
     9421 agacccgcac ggcgcgccag actgtggaaa ttcagaacgg caaaattgtt gccctgcgcg
     9481 agaacaatca gcatccggac gccacgctgc cgcactatga cgctggcggt aagctgatgc
     9541 tgcccaccac ccgcgacatg catattcatc tcgacaaaac cttctatggc gggccgtggc
     9601 gctcgctcaa tcgtccggca ggcaccacca tccaggacat gatcaaactc gagcagaaaa
     9661 tgctgccgga gttgcaaccg tacactcagg aaagggcgga aaaactgatt gatttattgc
     9721 agtcgaaagg cactaccatt gcccgcagcc actgcaatat cgaaccggtt tccggcctga
     9781 aaaatctgca aaatttgcag gcggttctgg cgcgacgtca ggcgggcttc gagtgtgaaa
     9841 ttgtcgcctt cccgcagcac ggtttgctgc tgtcgaaatc agaaccctta atgcgcgaag
     9901 cgatgcaggc gggggcgcat tacgtcggcg ggctggaccc gaccagtgtt gatggcgcga
     9961 tggaaaaatc cctcgacacc atgttccaga ttgcgctgga ctacgacaaa ggcgtcgata
    10021 ttcacctgca cgaaaccact ccggcaggcg tggcagccat caattatatg gttgaaacgg
    10081 tagagaaaac gccacagctg aagggcaagc tgaccatcag ccacgccttc gcgttggcaa
    10141 cgctcaacga acaacaggta gatgaactgg cgaaccggat ggcggcgcag caaatttcta
    10201 tcgcctcgac ggtgcagatc ggcacgctac atatgccgct taaacaactg cacgacaaag
    10261 gcgtaaaagt catgaccgga actgacagcg ttattgacca ctggtcgcct tacggcctgg
    10321 gcgacatgct ggaaaaagcc aatctctacg cgcagctcta tattcgtcct aacgaacaga
    10381 acctctcccg ttcgctgttt ttagctactg gcgatatatt gccgctcaac gaaaaaggcg
    10441 agcgtgtgtg gcctaaagct caggatgacg ccagctttgt gctggtggac gcctcctgtt
    10501 ccgccgaggc ggtggcgcgt atctcgccga gaaccgcaac gttccataaa gggcaactgg
    10561 tgtgggggaa tgtggcaggt tgatgcgcat ttgatatgtc tgcaacggtg aacagtaaga
    10621 gatttaagcc ccagggagtg gggcgggaat ggtttgttaa tgaatgatcc tgaactttat
    10681 aatctcccgg cataatttcc aggggattat ttgagagaaa tacacaaaag tgagttttaa
    10741 caaaggtatt tatcaagcca taaatgtgac ctgcttcgga taaaaaagat taaacatagg
    10801 aaaaccaatg atactcttgc tgatggttac caacagtaat taagatgtga ttttttccgt
    10861 aatcgaaaaa atattgccat gaccataaaa gaaaaattct cacaaaaata tccccatgct
    10921 tccttttgta cttttggcga ttcagcagcg ttggccgatc acctcgcaac gttgattgcg
    10981 actggtgtta aaacagcttc ctgtggttcg ctggcgggat gtattgaaga caacgcattt
    11041 ccgctgattg gtgagtataa gattgtagaa aatagccgtg gtgagcctgt ctgtgttatc
    11101 agagtgattg gcctgcattt actgcgcttt tctgatgtca ccgcagagct tgcccgtaag
    11161 gaaggtgaag gtgacctcag tcttgaatat tggcgtaatg aacaccgtcg ttttttccag
    11221 gcagaaggat gctattcacc agaaatggac gttatctttg aagaatatgc ccttattgac
    11281 gttgtgtaga taactatttt tctacgcgaa ataagctcac aaacataaag tatcgctaat
    11341 tttgtttatt ttgctgtttt ttacgtgatg attgtcacac ttattgttgc tgcccgtttt
    11401 gtccatattt ataaccaaat ttttaatagc tgcctgaaat agggctgttt attctgcgta
    11461 aaaatattcg aaaattctta ttctctccag cgcgctaatt tgggcgaaca cttcctgact
    11521 accctgcaat gaggctgaaa tgatgaataa acgttttgtt atcaatatgg tgtcttcgct
    11581 gttattaggc gctgcgctaa tttctgcgcc gctccaggca gcggaaaaag tcgtggtgaa
    11641 tatctctaaa gtcgatggta tgccgtggtt taatcgcatg ggcgaaggtg tggttgaggc
    11701 gggtaaagcc tttggtgtta atgcgtcaca ggtggggcct tcaagtactg atgctccgca
    11761 acaggttaaa attattgagg atttaatcgc ccgtaaggtt aatgcaatta ctatcgtgcc
    11821 aaacgatgcc aacgtgctgg aaccggtatt caaaaaagcg cgagatgccg gaattgtggt
    11881 tctgaccaac gaatctcccg gtcagccaag tgctaactgg gatatcgaaa tcattgataa
    11941 cgaaaaattt gccgctgaat atgttgagca tatggcgaaa cgcatggggg gcaagggcgg
    12001 ctatgtgatt tacgtgggta gtttgaccgt gccgcagcat aacttatggg ctgacttgct
    12061 ggtgaaatac cagaaagagc attacccgga tatgcatgaa gttacccgcc gcatgccggt
    12121 tgccgagagt gtggatgact cacgtcgtac cacactggac ctgatgaaaa cctatcctga
    12181 cttgaaagcg gttgtctcct ttggttcaaa cggtccgatt ggcgcgggac gtgcagtgaa
    12241 agagaaacgg gcgaaaaata aagtcgcagt ctatggcatg atgatccctt cacaggcggc
    12301 atcactgatt aaaagtggtg atatcaccga agggattact tacgatccgg cctctgcagg
    12361 ttacgcactc gcggccgtcg ccagcacgtt gctgaagggt gaagagatca aacctggatt
    12421 agaaatgcag aatctcggta aggccgacgt agatatggat aagcgaatta tccgtttcca
    12481 taaggtatta ctggtcaaca aagacaatat cgactcactt tattaatcca tttcttcggg
    12541 gcgaaagccc cgattcttat tgccagggga tgctatggaa accttccttt cccttcgtca
    12601 tatcaacaaa acgtttcatg ccacgcgagc gctgcgcgat gtttctctcg actttatgtc
    12661 gggtgaggtg cactgccttg ccgggcaaaa cggctgtggt aaatcgacgt tgattaaaat
    12721 tatgtccggt gtttatcgcc cggatgaggg ggcggaaatt acgcttggtg ggaaaaactg
    12781 gtcaaagctg acacccgccg cttcggtggc gcaggggatt caggtgattt atcaggacct
    12841 ctctttattt cctaacctga gtgtctggga gaatatcgcc gtgaatcact atcaccacgg
    12901 tctgtttgtt aaccgccgtc gtctgcgtga ggtggcgcag gcggcaatga ccagtattaa
    12961 cgtcacattg ccactggata cgctcgtttc tgagctttca attgcccgct gtcagctggt
    13021 ggcaatttgc cgtgcactgg cgcaggacgc acggttgatc gtgatggatg aaccaaccgc
    13081 ttcgctgacc catcaggaag tgcaagggtt attacaggtg gtgcatcaat tgcgggaacg
    13141 cgggatctgc gtggtcttcg tcagtcatcg tctggaagaa gtgatggaag tttccgaccg
    13201 tatttcagtg ctgaaagatg gtgagctggt cgggaccttt ccggccgcag agatgaccac
    13261 aaaacaactc agttttctga tgaccggcca ggagttcgaa tatcaggtac gagagttgtg
    13321 gcagggaaaa tccagcaccc cggtgctgga ggtgcgtaat ttaagtcgtc atggggaata
    13381 tcaaaatatt aacttgcggg tggaagcggg cgaagtggtg tcgattgttg gcctgcttgg
    13441 tgcggggcgc acggaattat gtctgagtct gtttggtatg acccgaccgg atgctggtga
    13501 gatcctcatc aacggtcagc cggttacact gcatagtaac caggacgcca tccgccacgg
    13561 tattggttat gtttcggaag atcgcatgtc gcgcggtttg gtgatggcgc aatctattga
    13621 agacaacatc attagcactg tttttcacaa ggtaaaagat cgcttcggct ttctgagtga
    13681 agctaaagtg cgtgacctgg tggacaggct aatcaaggca ttgaccatca aagtctctga
    13741 cccacatctc ccggtgaata cgctttccgg gggtaacgcg cagcgagtat cgatcgccaa
    13801 atggctggcg attgggccac gactgttgat tctcgatagc ccgactgtcg gtgttgatat
    13861 cgccaacaaa gcaggaatat acggcattat cagcgatctg gcggctcatg gcattgcggt
    13921 attgatgatc tgcgatgaaa ttgaagaagc gtggtatcaa agccaccgga ttttggtaat
    13981 gcaaaaaggt cagatcaccc atagcttcct gcctgacagc agttctcagg ccaggattgc
    14041 ggaggtggta aatggctgaa ctgaaaaaac gccatgaatt ctggctggca ttactgattg
    14101 tggtgctgtt tgttggcctt gcctggcgca gcgacgagtt tctgacattc ggtaatttgt
    14161 acgatctcgc caataactat gccatgttga ctattctcgc ctgtggtttg tttgtggtgt
    14221 taatttccgg tgggattgat atttcgtttc cggcaatgac catcattgcg caatacggca
    14281 tggtgctgtt gctgcaaaaa atgggtggca actttgctgt cgcgtttgcc ctggcgggcg
    14341 gcatcggcat tttacttggc ttaataaacg ccttactggt taatcgccta cgggtgccgt
    14401 ctatcatcat cactatctcg acgctgaata ttttttatgg cctgctgtta tggttgagta
    14461 aaggtgtgtg gctgtacgac tttccgccgt ggtttgagca gggggtaatg ttgttcaagt
    14521 acaccgatgc tgatggctat gactatggcc ttggtctgcc gctgatcgcc atgattacgg
    14581 tggtgctgct gacagcgttt atcatgaatt tcaccagtgt agggcgcaaa atttatgccc
    14641 ttggcgggaa ccgcgaatca gccagtcgca tcggctttag tgtgctgaaa ctgcaacttt
    14701 tcgtctatgg ctatatggga ttgatgtctg gtgctgcggg tgtagtgcag tcgtggacgg
    14761 tgatgactgt cgcccccgat tctcttctgg gttatgagct gacagtactg gctgcggtgg
    14821 tgcttggtgg cactagtttg ctcggcgggc gcggcacgtt aaccggtact ttgctcggcg
    14881 tggtgttgtt ggcagtaatg caaaacgggc taaatttatt gggagtctcg tcttactggc
    14941 aaacattgat cacaggcatc atcatcgttg ccagcattag cgccacggcg tggagtcagc
    15001 atcagaaccg gagtctgcta tgaaaaaatc gtggcgtaat aacgttgagt tttacttgat
    15061 cggcctgctg gtgctaactg ttgccgcgtt cagcattact atgccggaga ttttctggtc
    15121 gataagtaat ttccagtccg tcgcttcgca aatgcccgtg ctgggcattc tggcgttggc
    15181 tatggcggtg accatgcttt gcggtggcat taacttgtca attatagcca ctgctaacgc
    15241 ctgttcactg gtgatggcgt gggtggcgac tcagtatcca ccgggtattg ctacggttgt
    15301 cgctacgctg ctggcgggcg ctggcgcggc ggtgataatc ggtttatgta acggcgtatt
    15361 gattgctggt attcgagtgt cgccaattct tgcaacgctt gggatgatga cgttgctcaa
    15421 aggggtaaat attctggtga caggcggtag cgccattgcc aattatccct catgggtgct
    15481 gtggttgaat catgcacagt ggttcggtat tccgctaccg atgtggttgt ttaccgcagt
    15541 ggcgctgggg ctgtggatat tgctggagaa aacgccgctc ggtaaatcca tttatctgat
    15601 tggttctaac gaacgagcaa cactttactc cggtattaac actcgtcggg tgctgatatg
    15661 ggtgtatgtc atttccgcct tgctttgtgc ggtagcggca tttttgatga tgtcgaaact
    15721 caactcggct aaggcctctt atggcgaatc ttacttgcta gtgtcaatcc tcgcggcagt
    15781 actcggcggg gttaatccgg acggcggcag cgggcggata attggtatgg tgctggcgct
    15841 gtttcttctg caaattattg aaagcggctt caatattctc ggtataagtc cctacctgac
    15901 gatggcctta tggggaacgt tgctgctctg tttcattcag gctcgcggca tgttggggct
    15961 ggatcgggtg gtttaaatat tggctatatt caatggacgc gttttgccgc gatgacatat
    16021 caggcatcgc caaatacaca tagctaatca ggagtaaaca caatgaagat caaagctgtt
    16081 ggtgcatatt cagctaaaca accacttgaa ccgatggata tcacccggcg tgaaccggga
    16141 ccgcatgatg tcaaaattga aatcgcttac tgtggcgtct gccattccga tatccaccag
    16201 gtccgttccg agtgggcggg gacggtttat ccctgcgtgc cgggccatga aattgtagga
    16261 cgcgtggtag ctgttggtga tcaggtagaa aaatatgcgc cgggcgatct ggttggtgtc
    16321 ggctgcattg tcgacagttg taaacattgc gaagagtgtg aagacgggct ggaaaactac
    16381 tgtgatcaca tgaccggcac ctataactcg ccgacgccgg acgaaccggg ccatactctg
    16441 ggcggctact cacaacagat cgtcgttcat gagcgatatg ttctgcgtat tcgtcacccg
    16501 caagagcacc tggcggcggt ggctcctttg ttgtgtgcag ggatcaccac atactcgccg
    16561 ctacgtcact ggcaggccgg gccgggtaaa aaagtgggcg tggtcggcat cggcggtctg
    16621 ggacatatgg ggatcaagct ggcccacgcg atgggggcgc atgtggtggc atttaccact
    16681 tctgagttaa aacgcgaagc ggcaaaagcc ctgggggccg atgaagttgt taactcacgc
    16741 aatgccgatg agatggcggc tcatctgaag agtttcgatt tcattttgaa tacagtagct
    16801 gcgccacata atctcgacga ttttaccacc ttgctgaagc gcgatggcac catgacgttg
    16861 gttggtgcgc ctgcgacacc gcataaatcg ccggaagttt tcaacctgat catgaaacgc
    16921 cgcgcgatag ccggctctat gattggcggc attccagaaa cacaggagat gctcgatttt
    16981 tgcgccgaac atggcatcgt ggctgatata gagatgattc gggccgatca aattaatgaa
    17041 gcctatgagc gaatgctgcg tggtgatgtg aaatatcgtt ttgttatcga taatcgcaca
    17101 ctaacagact gaaaaaatta a
//
LOCUS       DAZUCC010000104        17112 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZUCC010000104 DAZUCC010000000
VERSION     DAZUCC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.104"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(241..1242)
                     /locus_tag="ACHY09_002870"
     CDS             complement(241..1242)
                     /locus_tag="ACHY09_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS3885822.1"
                     /translation="MSSWLAMLQDATEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            1349..1645
                     /locus_tag="ACHY09_002871"
     CDS             1349..1645
                     /locus_tag="ACHY09_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS3885823.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1768..2193)
                     /gene="arsC"
                     /locus_tag="ACHY09_002872"
     CDS             complement(1768..2193)
                     /gene="arsC"
                     /locus_tag="ACHY09_002872"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFS3885824.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(2206..3495)
                     /gene="arsB"
                     /locus_tag="ACHY09_002873"
     CDS             complement(2206..3495)
                     /gene="arsB"
                     /locus_tag="ACHY09_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFS3885825.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVILHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLFLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTSKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLTALALRLSFTL"
     gene            complement(3550..3903)
                     /gene="arsR"
                     /locus_tag="ACHY09_002874"
     CDS             complement(3550..3903)
                     /gene="arsR"
                     /locus_tag="ACHY09_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFS3885826.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLSLLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQVIVRN
                     LARQNCSADSKNICS"
     gene            complement(4151..4366)
                     /locus_tag="ACHY09_002875"
     CDS             complement(4151..4366)
                     /locus_tag="ACHY09_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3885827.1"
                     /translation="MMAISLQSEPPTQGWKVKNRRKVGFLHPDSDKAWYGSDPMKVYK
                     SSFTNMYSKLSLRQLKKSLTLAMLATG"
     gene            4645..4728
                     /gene="dinQ"
                     /locus_tag="ACHY09_002876"
     CDS             4645..4728
                     /gene="dinQ"
                     /locus_tag="ACHY09_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFS3885828.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4782..6134)
                     /gene="gorA"
                     /locus_tag="ACHY09_002877"
     CDS             complement(4782..6134)
                     /gene="gorA"
                     /locus_tag="ACHY09_002877"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS3885829.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6206..7048)
                     /gene="rlmJ"
                     /locus_tag="ACHY09_002878"
     CDS             complement(6206..7048)
                     /gene="rlmJ"
                     /locus_tag="ACHY09_002878"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFS3885830.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLSSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            7251..9293
                     /gene="prlC"
                     /locus_tag="ACHY09_002879"
     CDS             7251..9293
                     /gene="prlC"
                     /locus_tag="ACHY09_002879"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFS3885831.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKTVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILIRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            9301..10053
                     /gene="rsmJ"
                     /locus_tag="ACHY09_002880"
     CDS             9301..10053
                     /gene="rsmJ"
                     /locus_tag="ACHY09_002880"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFS3885832.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10102..11571)
                     /gene="dtpB"
                     /locus_tag="ACHY09_002881"
     CDS             complement(10102..11571)
                     /gene="dtpB"
                     /locus_tag="ACHY09_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFS3885833.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11886..12320)
                     /gene="uspA"
                     /locus_tag="ACHY09_002882"
     CDS             complement(11886..12320)
                     /gene="uspA"
                     /locus_tag="ACHY09_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFS3885834.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12711..13046
                     /gene="uspB"
                     /locus_tag="ACHY09_002883"
     CDS             12711..13046
                     /gene="uspB"
                     /locus_tag="ACHY09_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFS3885835.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13117..14616)
                     /gene="pitA"
                     /locus_tag="ACHY09_002884"
     CDS             complement(13117..14616)
                     /gene="pitA"
                     /locus_tag="ACHY09_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFS3885836.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14848..16041
                     /gene="yhiN"
                     /locus_tag="ACHY09_002885"
     CDS             14848..16041
                     /gene="yhiN"
                     /locus_tag="ACHY09_002885"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS3885837.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(16128..>17112)
                     /gene="yhiM"
                     /locus_tag="ACHY09_002886"
     CDS             complement(16128..>17112)
                     /gene="yhiM"
                     /locus_tag="ACHY09_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFS3885838.1"
                     /translation="GEFVLTGSGQSEYFVAGHVLISLSAICLALFTTAFIIISQLTHG
                     MNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMADEFVAGHVIFGVGMIAACVSTVA
                     ASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLIAVPVLLTLFGFIWSVILLRSADI
                     TPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTFSVKEHWLWCYWVILLGSLTIIFG
                     IYVLISSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSLANRIPMIPVFTC
                     LFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCFTLFSIVSILEAGSAKK"
BASE COUNT         4455 a         4337 c         4415 g         3905 t
ORIGIN      
        1 aatctgccct ttaaaagggg ggatagcact gaaacaaaga gacattgctt ctccgataaa
       61 atgtcagaga agcatgattg cgtggtgatt gtcgattgat aacttactga cggatccgtc
      121 atcattctca aagcattagt taagttattt gtgattttta aggattatcc tatctatgat
      181 tttctactca gaaggataag gcactcacaa tgctccttat agtttactgc tggcatctat
      241 ttaaaatagc atttgagtca aatagcccat caagatagcc atacctaata taaccgtcag
      301 gaatgcaact atcatcggta ttctgaacat tgatttaagt aatatcactt ccgtcaggct
      361 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
      421 cagaacagat gccagaggga taaccgcctc tgcccggata tatagcggaa taccgacaac
      481 ggcgctcaat gggatggcga agggattatc tgcccctgca tgagcggcaa tccactctga
      541 gggaataaag ccataaataa aagagcctat taatacccct aacaaaagat atggcagtac
      601 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttaccac
      661 agctttggta ctgcaacagt ttactacagg tttctcaatg gctttagccg ctggactaca
      721 gcaactcact tttattggcg tatatgtcgt ccccggcgaa gttttggctg gagcacaaca
      781 atttgctgat gagcttttac tggcaatgat atgacgttca aatcccagag aatcgaggat
      841 aatactggca agtacggaga cgccggcggc gataatcgca tacaacaagg taactttcca
      901 gccaaaggtc acccacatta acccgacgat aatgggatta agtaatgggg aaacaaataa
      961 aaaagtgagg gtcggaccaa acccggcttt cgctgataac aatccacgta acatagggat
     1021 tgtcgagcaa ctacagaacg gggtgacggc tcccaacaga gcagccagga gatagccttt
     1081 cccttttctt gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
     1141 gctcacaccg gcactaatca ctataaacag caaagaaagc tcgacggcga gaaacacaaa
     1201 catctctgtg gcatcttgca gcatcgcaag ccaactactc atacataacc tcattgaata
     1261 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
     1321 aaatatcaac cagacagagg gggatcatat gcagttggaa gaagtcgcta aagcgctgaa
     1381 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1441 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cccggctcta cactcagtca
     1501 ccatatttca gcactggtat cggtagggct ggtcaaacaa aaccgtgaga gtcgaacgct
     1561 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1621 ctgcgtaaat agcaaaattg cctgatgcgc tacgcgctac agatttcatg ccatgcacgg
     1681 ttttgtaggt cggataagac gcgttagcgt cgcatccgac ataaacaaag cgcactttac
     1741 caactatggg cagtggatat cgccgcttta tttcaagcgt ttacccgctt catcaaccac
     1801 tttctcgcca tcttccttgg tgaacgcgcc ttgttgcgca tctggcagaa tttccagcac
     1861 gacttctgaa gggcggcaca ggcgagttcc caacggcgtc accacaatcg ggcgattaat
     1921 cagaatcggg tgctgaagca taaagtcgat taaccgatcg tcagtaaatt tatcttctgc
     1981 aaggcccagt tcctcatacg gctcgacgtt tttacgcagc agcgcgcgta cggaaatccc
     2041 catatcggca atgagtttga ccagttcatc acgcgtcggt ggggtttcca gataatagac
     2101 aatggtcggt tctgtgccgc tgttgcggat catctccagc gtgttacgcg acgtgccgca
     2161 ggccgggttg tgataaatgg taatgttgct catatcaata tctcattaca aagtgaaaga
     2221 gagacgtagc gccagcgcag tcagcgtcac aaacagcaca ggcagggtca tgataatccc
     2281 tgtacggaag taatatcccc agctgatagt catattcttc tgcgaaagta cgtgcagcca
     2341 gagcagcgtc gccaggctac caatcggggt aattttcggc cccaaatcgc agccaattac
     2401 gttggcataa accatcgctt ctttgataac gccagatgcc gtgctgccat caatggacaa
     2461 cgcgccaacc agtaccgtcg gcatattgtt cataatagaa gagaggaagg cggtgaggaa
     2521 tccggtgccg agcgtcgcgg cccacaggcc attatccgcc agcacgttga gtacgccaga
     2581 aagatattcc gttagtccgg cattgcgcag gccataaacc accagataca tgccgagcga
     2641 gaagatgaca atctgccagg gcgcaccgcg tagcacctta ctcgtattaa tcgcatgacc
     2701 gcgtttagcg acgacaaata atatcaacgc gcccactgcc gcaatggcgc tgaccggaat
     2761 gccgagcggt tccaggacga aaaatcccac cagcagaaac agtaaaacaa cccagccagt
     2821 tttgaaggtg gcaggatctt taatcgcttc tgcgggagat ttcagcagcg ccatatcgta
     2881 gttctgcgga atatctttgc ggaaatagag atgcaaaatc accagcgtgg caacaattgc
     2941 ggcgatatcc accggcacca tcaccgatgc gtattcgcga aagccgaggc caaagaaatc
     3001 tgcggaaacg atattcacca ggttggagac aataagcggc aggctggcgg tatcggcaat
     3061 gaatccggcc gccatcacga acgccagcgt agtgccttta ctgaacccta aagccagcaa
     3121 catggcgatg acaatcggtg tcaaaataag cgccgcgcca tcattggcaa acagagctgc
     3181 aacggcagca ccgagcagga caatccaggt gaacagcaag cgaccgcgtc cattgcccca
     3241 gcgtgagacg tgcagcgccg cccattcaaa aaagccggac tcatccagca gcaggctgat
     3301 gataatgacg gcaataaacg ccgccgtcgc gttccagacg atattccaca ccaccggaat
     3361 atcacccgga tggaccacgc ccgtaactaa cgccagtact gcgccgagcg ttgcactcca
     3421 gccgatgcct aaacctttcg gctgccagat aaccaataca atggtcagga caaagatagc
     3481 gcctgccagt aacataatgc ctcccggata aaacacatgc gaaaatacat atgtgtttag
     3541 ctaaattttt taactgcaaa tgttcttact gtctgcggaa caattttgtc gagccaggtt
     3601 gcggacaatc acctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc
     3661 cgcccatgct ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcgatc
     3721 cagcaatagc ccactttcac gcagcaatga cagatggcgg gatatcttgg gctgcgactg
     3781 atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa gcagtaaaac
     3841 gatgcccaga cgggtctcat cagcaagaat tttgaacagt tgaatgggta acagaaatga
     3901 catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata agtcaaaaac
     3961 atatatggtt taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg
     4021 ggtattcatt tgaaaggagg tctgaatcag gacgtaaaaa aaccgacttt gcgtcggttt
     4081 tttactttcc agccctgagt tggtggctct ggctggagtg agaatgccca catctaacgg
     4141 ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc agctggcgga
     4201 gggacagttt gctatacatg ttggtgaaag aggatttata tactttcatc gggtcgctgc
     4261 cgtaccaggc cttgtccgaa tccggatgta aaaaaccgac tttgcgtcgg ttttttactt
     4321 tccagccctg agttggtggc tctgactgga gtgagatagc catcatctaa cgcatcaatg
     4381 ctaataccat actgaaacta ttgcaaggac atgctggttt tataacccgc aagtactgta
     4441 tgattatcca gttagctctg agacattttc actctggcaa tgcgcataaa cgctttcaaa
     4501 gtcctggtca gaagtacggg tggtgccgtt aactgatgct ctggccggag tgagaatgcc
     4561 cacatctaac aatggggcat gcgccgtgac aggcagtgga tgagtaagcg gatgcattct
     4621 cactccatcg catggagaaa acgggtgatt gataaagcaa tcatcgttct tggggcgtta
     4681 attgcgctgc tggaactgat ccgctttctg cttcagcttc tgaactgata gcggaaacgt
     4741 agctaagggc taagagcaca ctactcttag ccctttaaca tttaacgcat tgtcacgaac
     4801 tcttctgccg ctgtcgggtg aatggcaacc gtgttgtcga agtctttttt ggttgcgccc
     4861 atcttcagcg ccaccgcgaa gccctgcaac atttcatcca taccaaaacc aatgccgtga
     4921 attccgacaa tcttctcttc cggtccaacg cataccagtt tcatgcggca cggctggcgg
     4981 tgagtggtga cggcggtata catcgcggtg aaagaggatt tatacacttt cacctgatcg
     5041 tcgccgtact gctcgcgcgc ctgcggttcc gttaaaccaa cagtcccaat cggcggatgg
     5101 ctgaagacca cggtcggaat gttgctgtaa tccagatgct catccggctt gttattaaac
     5161 aggcgttcag agagacggcg acccgctgca actgccaccg gtgtcagttc cactgcaccc
     5221 gtgttatcgc ccaccgcgta aataccttca acgttggtat tttgatattt atcgacgacg
     5281 atatagcctt tttcgttagt tttaacgcca gcggcttcca ggttgatgtt gtcattggca
     5341 ggctcgcgac caatcgccca aatcaggcaa tccaccgttt cactgcgccc atcttccagc
     5401 tccagcgtca ggctaccatc agcatttttc actactgctt tcgggatggc gttggtatgc
     5461 aactgcgggc cttcggcgtt catcacttcg accagcgttt cagagatcat cgggtcgaag
     5521 ctgcgcagcg gcgcatgttt acgcacaaac agatgcgttt tcgcgccgag gccgttaatc
     5581 acgcccgcca gttccacggc gatgtaacct gcgccaacaa ccgccacgcg ctctggcaaa
     5641 gcaggaaggg cgaagaagcc atcagaatca ataccgtatt ccacgcctgg aatatccggg
     5701 tggctcggac gaccgccggt ggcgatcaga atatgatcgg ccgtgatggt ttcgccgttt
     5761 acctccagcg ttttggcatc aacgaagcgg gcaaagcctt tgattacatc aacgttattt
     5821 ttaccgagca cgttttcata ggaagtatga atacggtcga tataggcggt acggctggcg
     5881 atcaacgttt cccagttgaa tttattgata gtggtatcga aaccataatc cggaccgtac
     5941 atgtggatcg cttcacggat ttgcgccgcg tgccacatca cttttttcgg cacgcagccg
     6001 acgttcacgc aggtgccgcc cagttctttg gcttcaatca gcgcgcattt ctgaccgtac
     6061 atagccgcgc ggttgatgga ggcgataccg ccgctgccgc cgccgatggc gatgtaatca
     6121 tagtgtttag tcatgacata ttgtccttat cgttgattac cgcgattgta gcgcgagacg
     6181 taataggtgc cagcgatggc tgcaattact ccggcacgat ccagcttgcg gtggcgtgcc
     6241 cagtgccagc tggaaccagt ttgctgtgca gccacggcag cacgttattc atctgctgtt
     6301 ccagcttcca cggcggatta atcacaatca tgccggaagc ggtcatgcca cggcgatcgc
     6361 tgtctggcag caccgccagt tcaatttgca gaattttgcg aatgccggtc gcttccaggt
     6421 cgtggatcat gcgcttaatt tgctggcgca gcaccactgg ataccacaat gcgtaagtgc
     6481 cagtggcgaa acgtttgtaa ccttctgcta tcccgctgac caccgcctga tagtcggttt
     6541 tcatttcata cggcgggtcg ataaggatta aaccacggcg ggaaaccggc ggcagcttgg
     6601 ccttaagctg ctggaaaccg tcggcttttt cgacgcgcgc acggctgtct ttctgaaatt
     6661 cagaacgcag caacgggtaa tcgctcgggt gcagctcggt cagttgcagg ctgtcctgtt
     6721 cacgcagtag ctggcgagca atcaacggcg aaccggggta ataacgcaac tgcccgctac
     6781 ggttgaagtg ttttaccaca ttgatgtacg cctccagctc cgcaggcaaa tcgtcctgct
     6841 gccagatacg ggcgatacct tcgagatatt cgccggtacg ctcggcatgt tcgctgctta
     6901 actgataacg cccggcccct gcgtgggtgt cgagatagag aaacggttta tctttctctt
     6961 tcagcgactc gatgatcagg ctctgaacgg tatgtttaag gacgtcggcg tggttgccag
     7021 cgtgaaagct gtggcgataa ctgagcatgg gtaaaggtgt tccggtaagt aaaaatcggc
     7081 ccgtattcgg ggcgcacaaa agcctatcag gacagtatac cgaaaagagg ccgccgccgc
     7141 gaaagcgtaa cgtttctcat tgaaattcac tacacttaac cccatgctac acacattatg
     7201 taaagcgcct gttgagcgct tccttaacct ctttaaccag gactgcgcta atgacgaatc
     7261 cgttactgac tccctttgaa ttgcctccgt tttctaaaat tctcccggaa catgtcgttc
     7321 cagccgtgac taaggcgctg aatgactgcc gcgaaaacgt ggagcgcgta gtagcgcaag
     7381 gggcaccgta cacctgggaa aatctctgcc agccgttggc agaagtagac gatgtgctgg
     7441 ggcgtatctt ctccccggtc agccacctga actcggtgaa aaatagcccg gaactgcgtg
     7501 aagcgtacga acaaaccctg ccgctgctgt cagaatacag cacctgggta gggcaacatg
     7561 aagggctgta taaagcgtat cgcgacctgc gcgatggcga tcattacgcc acgctgaaca
     7621 cggcgcagaa aaaagcggtt gataacgcac tgcgcgactt cgaactctct ggcatcggtc
     7681 tgccaaaaga gaaacaacaa cgctacggtg agattgccac ccgtctttcc gagctgggca
     7741 accagtacag caacaacgtc ctcgatgcga caatgggctg gaccaaactc gttaccgacg
     7801 aagcggaact ggcaggaatg ccagaaagcg cactggctgc ggcaaaagcc caggctgaag
     7861 cgaaagagct ggaaggttat ttgctgacgc tggatatccc aagctacttg ccggtaatga
     7921 cttactgcga caaccaggcc ttgcgtgaag agatgtatcg tgcttacagc acccgcgctt
     7981 ccgatcaagg cccgaacgcc ggtaagtggg acaacagcaa ggtgatggaa gagatcctcg
     8041 cgctgcgtca cgaactggcg caactgctgg gttttgaaaa ctacgccttt aaatcccttg
     8101 ccactaaaat ggcagaaaat ccgcagcagg tgcttgattt cttaaccgat ctggcaaaac
     8161 gcgcgcgtcc gcaaggtgaa aaagagctgg cgcaactgcg tgccttcgct aaagccgaat
     8221 ttggcgtcga tgagttgcag ccgtgggata tcgcttacta cagcgaaaaa caaaaacagc
     8281 atctctacag catcagtgac gaacagctgc gtccgtactt cccggaaaac aaagcggtta
     8341 acggcttgtt tgaagtggtg aaacgtattt acggcatcac cgctaaagag cgtaaagatg
     8401 ttgatgtctg gcatccggat gtacgtttct tcgaactgta tgacgagaac aacgaactgc
     8461 gcggcagctt ctacctcgac ctgtatgccc gtgaaaacaa gcgcggcggg gcgtggatgg
     8521 atgactgcgt aggccagatg cgtaaagccg acggttcgct gcaaaaaacg gtcgcgtatc
     8581 tgacctgtaa cttcaaccgc ccggtaaatg gtaaaccggc actgtttacc catgacgaag
     8641 tgatcaccct gttccatgaa ttcggtcacg gcctgcacca tatgctgacc cgcattgaaa
     8701 ctgctggtgt atctggtatc agcggggtgc cgtgggatgc ggtcgaactg ccgagtcagt
     8761 ttatggaaaa ctggtgctgg gagccggagg cgctggcgtt tatctccggt cactatgaaa
     8821 ccggcgaacc gctgccgaag gagctgctgg ataaaatgct ggcggctaag aactaccagg
     8881 cggcgctgtt tattctgcgc caactggagt tcggcctgtt tgatttccgc ctccatgccg
     8941 agttccgccc ggatcaggga gcgaaaatcc tcgaaactct ggcagaaatc aagaaactgg
     9001 ttgccgtagt accgtctcca tcctggggcc gtttcccgca cgctttcagc catattttcg
     9061 ccggtggtta tgccgcaggt tactacagct acctgtgggc cgacgtgctg gcggcagatg
     9121 ccttctcgcg ttttgaggaa gagggcattt tcaaccgtga aactgggcag tcgttcctcg
     9181 acaacattct gatccgtggc ggttcagaag agccgatgga gctgttcaaa cgcttccgtg
     9241 gtcgtgaacc acagctggat gcgatgctgg agcattacgg cattaagggc tgaacattca
     9301 gtgaaaatct gcttaattga tgaaacaggc gccggagacg gcgccttatc tgttctggcg
     9361 gcccgctggg ggctggagca cgatgaagac aacctgatgg cgctggtgtt aacaccggag
     9421 cacctggaac tgcgcaagcg tgatgagccg aagctgggcg gcatctttgt tgattttgtt
     9481 ggcggggcga tggcgcaccg acgcaaattc ggcggtggtc gcggagaggc ggtggcgaaa
     9541 gcggtgggca ttaaaggcga ttatttgccg gatgtggtgg acgccaccgc agggctgggg
     9601 cgcgatgcct ttgtgctggc ttcggtcggc tgccgcgtgc ggatgctgga gcgtaatccg
     9661 gtggttgctg cattgctcga cgacggcctg gcgcgtggtt atgcggatgc ggaaatcggc
     9721 ggctggttgc aggagcggtt gcagttaatt cacgcttcca gcctgacggc gctgaccgat
     9781 attactccgc gcccgcaggt ggtctatctc gatccgatgt tcccgcataa gcagaaaagc
     9841 gcgttggtga aaaaagagat gcgtgtcttt cagtcgctgg tggggccgga tcttgatgcc
     9901 gatggattgc tggagcctgc acgcttgctg gcgaccaaac gcgtggtggt aaagcgcccg
     9961 gactacgcgc cgccgctggc gaatgtcgcc acgccaaacg cggtagtgac caaagggcat
    10021 cgctttgata tttatgccgg aacgccggtg taaaagtaaa gcagggtagc gttatcgcta
    10081 ccctgctttc tgcaagaata attaatggct ttcgggcgtc gcaatcatgc gtttcagcca
    10141 cggcaccatc agcagcatca ccaccgcaac gcccagcgta accaggccaa tcttaccaaa
    10201 cacgttggta tagacgggca acgtctcaag cggatcggta atgttgtctg gtactgcggt
    10261 aaatgttgcc acatagccgc ccagcaagaa cgcggcagcc tgcgtcagga accacatccc
    10321 gagaataaag cccatcaaat gctgcggcac cagggcagca atcatcgcca gacccagggc
    10381 gctaataaac aattcaccca ggctctggaa caagtaaacc agcacgataa accacggcga
    10441 tgtcagtcct tgtgcatcag caaaccacat tcccgcagcg gcggctgtaa gaaagcccag
    10501 cgagcacata aacatgccga gagtaaattt catcggcatc gagaggtctt tgcctttgtt
    10561 acccagatgc gtgtaaatgc ctgccagtat tgggctggcg agtaccaccc agaacgggtt
    10621 aagcgcctgg aagctgactg ggttgatgga aaagccgaga atttcatgat gcacgttgtt
    10681 gatagcaaag aagttcagcg atgttggcat ctgggcgtag agaatgtaaa acaccaccgc
    10741 ttcgagcatc aggacaaagg cgacaaacat tttattgcgc ccggttttat ccagcttgaa
    10801 tgcctgacga aagaaaatga tggtgacgac gatggagaga acaatcagca ccagattggc
    10861 gacttctacg ttgtgcatca gccatgcaca gacgaagatc atcaccacgc tgccaagtaa
    10921 cacgtacaac agtttgctga agctcatcgg ccggaagtcg ggttcagaac caatgtcttt
    10981 caccattccg cgacaggcga tgtaaaccaa tagcgcgata attaatcccg caccgcacag
    11041 gttgtaggtg actgaatagc cgaatctatc agcgatcaca ggggccagcg ataacgctat
    11101 caacgagccg atgttgatcg acatatagaa cagggtaaat gcgccatcaa gccgcggatc
    11161 tttcggcgga tagcacttcg aaagcaagct ggctgggtta gctttaaaca ggccgttacc
    11221 gacagcgata gtccccaggg cgatgaaaat caggtcaggc ttaagtagcg acaggccggt
    11281 catgaagtag ccaatcgcca gcacaagtgc tccgaggaca atggtgcgtt tggtccccag
    11341 caggtggtcg ccgacatagc cgccaatgga aatgaggcca tagaccagcg cagcaaaagc
    11401 accaaaagtg acaaaagcct gttcttgcga gaatccaagc tgcttaacga agaaaaccgc
    11461 cagtacgccc tgcacgccgt agtagccgaa tcgctcccat aactcgacaa aaaagatcat
    11521 gaaaaatggg cgaggttgct gcagcatccc catgggtgtt gttgtattca tatttattaa
    11581 ccttccaata ccatcctgaa aagtatgaac gcgtaaggga taacgcgcga aaaagtttac
    11641 atctgtcaga gattcttaat attgatgcgg tgaatagatt aatacactat tacaacagaa
    11701 aataaccagc tgatcacact aatgatggaa aatatgatgg tgttaaatgt tgtattggca
    11761 ggggattttg attgcgtacg cagaccgtgg ccagataagg tgtttacgct gcaaggtaac
    11821 aaggcgcgcc aaaaagcaaa acgcccgcat atgcgggcgt tcaggaacac gtcgtagagg
    11881 gaagattatt cttcttcgtc gcgcagcgga acaatcagca tatcaacgtg aacggtgttg
    11941 atcagctgac gtgcggaaga catcagtttg ctccagaagt cctggtggtg accacaaacc
    12001 accaaatcca tatcgtattt cttgattgca tcgaccagaa cctggcccag gtcgccgctg
    12061 ccgctcaggg tttcagtgat tgggtagcct gcattagtgg aaagctcggt cagcgcatga
    12121 tgtgtctctt cagagatgcg tttctgcata tcacccagat tcacatcaat aagcccggtg
    12181 tataggtcag agtagtttac atctacgtgg atcagagaaa ctttcgcatt gtaggggcga
    12241 gccatagaga ctgctttctc taccagaact ttgctttccg gggagagatc gaccgcgatg
    12301 agaatgtgtt tataagccat agtgttactc cttccataaa gttgtcgatg actggccagc
    12361 tagcgtttct tgtgccgtta cgtcacccgc gtcctgcgaa ctacgtgcgc ggggctaacc
    12421 cagccgtaaa gattcagtgt taagaccatc cttaccttat agcgacccgg atgatccgtc
    12481 aatccgcctt gcttacccgt tagataaaca aattttcgac atgaatatat tgatagtggt
    12541 taaccttctg gaaaaaaaac aacctgatct cctacactat ctatagagcc gctcggatgt
    12601 tggtcaccac aaaagcggtt tctccaggac ctggtgttaa ctgattggca gcgtatcggg
    12661 agcggtagcc ccggggtgga actccgcggg caggtcgccg gggaggaggt atgataagca
    12721 ccgtcgcatt attttgggct ttatgtgtcg tttgcattgt taacatggcg cgctatttct
    12781 catcactacg cgcgttgtta gtggtactgc gtaactgcga tccattgctc tatcaatatg
    12841 ttgatggagg gggctttttt acctcacatg gccaacccaa caaacaggtg cgtctcgttt
    12901 ggtatatcta tgcccaacgt tatcgcgatc atcacgatga tgagtttatt cgccgctgtg
    12961 agcgggtgcg tcggcagttt attctgacca gcgcattgtg tggtctggtg gtggtcagtc
    13021 tgattgcatt gatgatttgg cattaagcac ttcagcaaaa ataacgcata aaaaaagcgg
    13081 gccagtaaac tgacccgctc gttttggtgc gtgcgattac aggaactgca aggagagcca
    13141 gtacagcccg ccggaaagca gtaccgcagc cggaagggta aacacccagg ccatcagaat
    13201 actggtcacg gttttacgct gtaagccgcc gccatctacc accatcgtcc ccgcgacaga
    13261 agaggagagt acgtgggtag tggaaaccgg catcccggta taactcgcca ggccgataga
    13321 cactgccgca gtcatctggg cagacattcc ctgagcgtag gtcatgcctt tcttaccgat
    13381 tttctcaccg atagtcgttg ccacacgacg ccagccaatc atcgtaccga tacctaacgc
    13441 cagcgcgacc gccatgatga tccacaccgg tgcatactcg atggtgctaa gcatgtcaga
    13501 cttcagtttc ttcaacaggc gctgatcgtc agcactcacg ccaggcatct tcaccacttt
    13561 gtcgatagtg tcagaaacgc acagcataat gcggcgcatc tggctacgtt gatcaagcga
    13621 cagcttatcg tagctttcca catcggtagt caacatgcct ttcaggcggt tgagcgcgtt
    13681 aatggtattc gacggatggc aatggaactc cgcaggttgc gttgcgccag cttccggagc
    13741 cggtactaac tgatcagcac cggtcgcttg tttcagcagc gcaggatgct gctcaaagta
    13801 agcttcgacg ttgttgatgg catcacgggt acgggtgatt tcgtagccag tggcattcat
    13861 gttcaccacg aagcctgctg gtgcgacacc aatcaatacc aacataacca gaccaatgcc
    13921 tttctgacca tcgttcgcgc catgcgaaaa cgccacgccg atagcggaaa ggatcagcgc
    13981 aatacgcgtc cagaacggcg gctttttctt gccgtctttc ttttcacgct ccgctggggt
    14041 caggtggata cgggcgcgtt tcttggtgcc gctccagtag cgacgcagca agaaaatcag
    14101 accgccagca aacaccaggc cgacaatagg ggaaacgatc agagaaccga aaatacttaa
    14161 tactttcggg atattgagtg catccaccac tgacgtcccg gtcatcaacg cattggttaa
    14221 accaatcccg atgatcgcgc caatcagcgt atgagagctg gatgcaggta aaccaaagta
    14281 ccaggtaccc aggttccaga taatcgccgc cagcaacata gagaacacca tggcaaggcc
    14341 atgagacgat cccatattaa gcagcagatc cgtcggcagc atatgcacaa tggcataggc
    14401 aacactcaga ccacccagca aaacacccaa aaagttgaac accgccgcca taaccacggc
    14461 gagctgagaa cgcatcgcgc gggtatagat aacggttgcc acggcgttgg ctgtgtcatg
    14521 gaaaccattg atggcttcgt agaacagcac aaaagccagt gcaagcaata ataacagccc
    14581 ggtatgcaaa tccaggccag caaacaaatg tagcatagga cgttacgcca ttttgaggac
    14641 atgaacgcgg cgcattatca gtgactttcg cggcgcgggc aaagtgaaat atagactttt
    14701 tttgatttgc ctcctgtatg gatttcactc aaaaaataat tatcttatat aattcaggta
    14761 aatacttcct tttagtaata ttgatgctgg tgcgaccact gaggaatctt tacaattcac
    14821 gcccgttttt tcttagagga gcgcaaagtg gaaaggtttg atgccattat tataggcgct
    14881 ggtgcggcgg gtatgttctg ttctgcgctg gcaggtcagg caggacgccg ggttctgctg
    14941 atcgataatg gtaaaaaacc agggcgcaaa atcctcatgt ctggcggtgg gcgctgcaac
    15001 tttaccaacc tttatgtcga accgggcgct tatctgagcc agaatccgca tttttgtaag
    15061 tctgcactcg cgcgttttac ccagtgggat ttcattgatc tggtcaataa acacggcatc
    15121 acctggcacg agaaaacgtt agggcaactc ttctgcgatg actccgcgca gcagattgtc
    15181 gacatgctgg tggatgagtg cgagaagggc aatgtgacct tcagattgcg tagcgaagtg
    15241 ctgagtgtgg cgaaggatga aacaggcttc acgcttgaac tgaacggcat gactgtcggt
    15301 tgcgaaaagc tggtcatcgc gaccggtgga ctgtcaatgc cagggctggg cgcgtcgccg
    15361 tttggttata agattgccga acaatttggc ctcaacgtgc tgccgacccg cgcgggcctg
    15421 gtgccattca ctctgcataa accgttgctc gaagagttac aggtgctggc gggcgtggcg
    15481 gtgccttccg tgattaccgc tgaaaacggc atcgttttcc gtgagaactt actcttcacc
    15541 caccgcggct tatctggacc ggcggtgttg caaatttcca gctactggca accgggtgaa
    15601 tttgtcagta tcaatctgct accggatgtg gacctcgaaa ccttcctgaa tgagcagcgt
    15661 aatgcacatc cgaatcagag cctgaaaaac acactggcgg ttcatctacc gaagcggttg
    15721 gttgaacgtt tacaacaact cgggcaaatc ccggatgttt cgctaaaaca gctcaacgtg
    15781 cgtgaccaac aggcactgat tagcacattg accgactggc gcgtacaacc caacggcact
    15841 gaaggctatc gcactgccga agtgacgctc ggcggcgtgg acaccaacga actctcttca
    15901 cggacgatgg aagcgcgcaa agtgcctggg ctgtacttca tcggcgaagt gatggacgtc
    15961 accggctggc tggggggcta taacttccag tgggcgtggt cgagtgcatg ggcttgtgcg
    16021 caggatttgg tggcagaata agaagcaaaa tgtgctctcg ataccagagg gattaacctg
    16081 aataaaacca gataaggcgt ccaacgcctt atctgaagcc ttttttatta ttttttcgct
    16141 gaacccgctt ctaatataga aacgatggaa aacagtgtaa aacaaactgc acctaagcca
    16201 accagaactc gcgaaggaat aaagtaagcc atatcggttt gcgcaatttc agccaggaac
    16261 gcagcaagga acagacaaaa cagacaggta aataccggaa tcatgggtat tctgttggct
    16321 aacgaacagg tgcgtcgcca taccagcgct aacaaccaca ctttggagaa aatactgtag
    16381 cagatcatcc ccaggcaaat gagaataatg ccgggggcca ggcgggcgct tgcatctgaa
    16441 ctgattaata cgtagatacc aaaaataatg gtaagtgagc caagcaaaat aacccaatag
    16501 caccacagcc aatgttcttt cactgagaac gtattccgcg tttgatggac aatagtggcg
    16561 acaaggccaa taagacaagc gcaaattgcc gttaacccca ttagtacgtg acctgcaaca
    16621 taatgtggtg tgatatctgc actacgtaac agtatgactg accaaatgaa accaaagaga
    16681 gtaagcagaa cgggaacggc aattaagcaa ttaccgattg ttgaagaata ggcctgcaat
    16741 ggcgtaccgt cactcttact gcccgaggcg tttttgggga tcaacagaaa atgtccggaa
    16801 gatgccgcta cggttgatac acaagcggca atcatgccaa cgccgaaaat aacatgaccg
    16861 gcgacaaatt catctgccat cacattattg cttgccaaca gtgaccatcc ccaaatcata
    16921 gttgtagcag aaccggcata accaataacg ggaaataacc tgttgtagaa tttattcatg
    16981 ccgtgagtta actgagatat aataataaat gcggtagtga acaatgccag acatatcgca
    17041 gaaagagaaa tcagaacatg ccctgcaacg aaatattcac tttgcccaga accggttaat
    17101 acaaactcac ct
//
LOCUS       DAZUCC010000105        17055 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZUCC010000105 DAZUCC010000000
VERSION     DAZUCC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.105"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(44..1501)
                     /gene="nuoN"
                     /locus_tag="ACHY09_002887"
     CDS             complement(44..1501)
                     /gene="nuoN"
                     /locus_tag="ACHY09_002887"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFS3885839.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(1508..3037)
                     /gene="nuoM"
                     /locus_tag="ACHY09_002888"
     CDS             complement(1508..3037)
                     /gene="nuoM"
                     /locus_tag="ACHY09_002888"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFS3885840.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGFWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3201..5042)
                     /gene="nuoL"
                     /locus_tag="ACHY09_002889"
     CDS             complement(3201..5042)
                     /gene="nuoL"
                     /locus_tag="ACHY09_002889"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFS3885841.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5039..5341)
                     /gene="nuoK"
                     /locus_tag="ACHY09_002890"
     CDS             complement(5039..5341)
                     /gene="nuoK"
                     /locus_tag="ACHY09_002890"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFS3885842.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5338..5892)
                     /gene="nuoJ"
                     /locus_tag="ACHY09_002891"
     CDS             complement(5338..5892)
                     /gene="nuoJ"
                     /locus_tag="ACHY09_002891"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFS3885843.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(5904..6446)
                     /gene="nuoI"
                     /locus_tag="ACHY09_002892"
     CDS             complement(5904..6446)
                     /gene="nuoI"
                     /locus_tag="ACHY09_002892"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFS3885844.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6461..7438)
                     /gene="nuoH"
                     /locus_tag="ACHY09_002893"
     CDS             complement(6461..7438)
                     /gene="nuoH"
                     /locus_tag="ACHY09_002893"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFS3885845.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7435..10161)
                     /gene="nuoG"
                     /locus_tag="ACHY09_002894"
     CDS             complement(7435..10161)
                     /gene="nuoG"
                     /locus_tag="ACHY09_002894"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFS3885846.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10214..11551)
                     /gene="nuoF"
                     /locus_tag="ACHY09_002895"
     CDS             complement(10214..11551)
                     /gene="nuoF"
                     /locus_tag="ACHY09_002895"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFS3885847.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEVGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(11548..12048)
                     /gene="nuoE"
                     /locus_tag="ACHY09_002896"
     CDS             complement(11548..12048)
                     /gene="nuoE"
                     /locus_tag="ACHY09_002896"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFS3885848.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12051..13853)
                     /gene="nuoC"
                     /locus_tag="ACHY09_002897"
     CDS             complement(12051..13853)
                     /gene="nuoC"
                     /locus_tag="ACHY09_002897"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFS3885849.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(13947..14609)
                     /gene="nuoB"
                     /locus_tag="ACHY09_002898"
     CDS             complement(13947..14609)
                     /gene="nuoB"
                     /locus_tag="ACHY09_002898"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFS3885850.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(14625..15068)
                     /gene="nuoA"
                     /locus_tag="ACHY09_002899"
     CDS             complement(14625..15068)
                     /gene="nuoA"
                     /locus_tag="ACHY09_002899"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFS3885851.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(15698..16636)
                     /gene="lrhA"
                     /locus_tag="ACHY09_002900"
     CDS             complement(15698..16636)
                     /gene="lrhA"
                     /locus_tag="ACHY09_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFS3885852.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4202 a         4828 c         4447 g         3578 t
ORIGIN      
        1 ccgccgaaca tgtcggcggc tttctgactt acaaactgac tgcttacatc agcggcattg
       61 ctaaccgcac aatgctaatc agcggttgtg gccatacacc cagcaccagt accaacagtg
      121 cagagatcag caccacgata ccgcctgcgc tgtactgcca gtttgatggt gcatcgcgac
      181 ccggttgttc cggagcgtga aggtataagc tcaccgccac gcgcaggtag tagtagaggc
      241 cgattgccga accgacaact acggcaccca ccagccacca caagtgtgcc tggacaccga
      301 ccgccagcac gtagaactta ccgataaagc ccagcgtcat tgggatacca gccagtgaaa
      361 gcatcatcac cgtcatcact gccgcgagga tcggacgatg ccagaacaga ccgcggtaag
      421 agaacaggga atcagcatcc gggccacgat acgggctgga catcaggctg accacgccga
      481 acgcgccgag gctgctgaac agataacctg ccaggtaaac ccctaccgct tccatcgaca
      541 tctcgccagt ttgcagcgca atcagcgcca ccagcagata gccgaggtga gagatagatg
      601 agtaaccgag cagacgtttg atattggtct ggctcagcgc catcaggtta ccgaagatga
      661 tggaggcaaa ggcgataatc gccagcacca cgcgaatcgc ttcgctgtca cccaccggtg
      721 cgtagaggaa cagacgcatc accacaccga agatagcgat tttgctcgcc gtcgccagga
      781 aagtggaaac cggcgcaggc gcgccctggt atacgtctgg cgtccacagg tggaacggca
      841 ccagagagag tttgaagccg aggccgacaa tcatcaggcc gaaacctgcc agcaacagcg
      901 gctcgttgag cataccgtcg ccgaggtttt tacccagcgc gacaaacgac aggtcgccag
      961 actgcgcata caccagcgcc ataccgaaca gcaggaaaga agacgctgcg gcagagagaa
     1021 tggtgtattt gatactggct tccagcgaac gtttctggcg gaaagcgtaa ccgaccaggc
     1081 caaacagcgg caaagagatc agttcgatac cgaggaacag agacgccaga tggttggcat
     1141 tcgccagcag gatcccgccc agggcggcaa ttaacaccaa caggtagaac tcgtccttgt
     1201 tgtcgttata gccttcaagc cacgggtagg cgaaagtaca ggtggcgagg ctcgccaaca
     1261 ataccagccc ggtgtaaagc atggcgaaac catcaacgcg catcagtggc gtaacgtcca
     1321 tagcgcccgc ctggccgaca aaccagagcg aaaccagcgc cgcgttaagc ccaataaccg
     1381 agagcgtagc gttgaggaaa tgattgcgtc gccacgcaat ggagagcatc accaccacca
     1441 ccgtcaagcc gacgatcagc aacggtagca gtgcgatcag gttttgtgga gttattgtca
     1501 tggcgattta cggccttgta gtagtaacgg aattaacaaa ccactgctgg atattgccaa
     1561 tcgcggagtg cgaggtatcc agaatcggct gcggatagaa gcccagcagt accagcagca
     1621 ccaccagcaa caggatcata aacagctcac gcagcgacat ccctggcagt tcctggctgg
     1681 caatctggct tttcgcttta ccgaagtaag cgcgatgtaa catcgccagc gaataaacag
     1741 atgcaaagac cagcccaaag gtagagataa cggtaatcac cgggacaacc tggaagctgc
     1801 cgaacagaat cataaattcg ccgacgaagt taccggtgcc aggcatccca agcgttgcca
     1861 ccgcaaagaa cagcgacagt gctggcagcc atttcatctt gctccacaga ccgcccatca
     1921 tgcgcatgtc gcgggtatgg atacgttcat aaagctgacc gcagagaata aacagacccg
     1981 ccgccgacaa gccgtgcgca atcatctgga ttaccgcgcc ctggtaggcc aactggctgc
     2041 cagtgtagat agcaatcagc acgaagccca tgtgggaaac cgaggtgtag gcgatcagac
     2101 gtttgatatc ggtctgggcg aaggccatcc acgcaccgta gaagatgccg ataacaccca
     2161 gccacatagc gattggcgcg aactctgccg acgcgttcgg gaacaacggt agggagaaac
     2221 gcagcagacc gtaagcggcg gttttcagca agatccccgc gaggtcaacg gaaccggcgg
     2281 ttggagcctg tgagtgcgca tccggcagcc agccatgcag cggaaccacc ggcattttca
     2341 ccgcaaatgc gatgaagaag cccagcatca acagatattc cacaccactg gacattggcg
     2401 tattcaacag ctcttcatag ttgaaggtcc agaagccggt cgcattgtag tgaacaaaaa
     2461 ccagcgccag gatggcgatc aacatcacca gaccactcgc ctgggtgtaa atgaagaact
     2521 tggttgccgc cgtgatacgc gttttaccgt cagaggcttt atgcccccac agtgcgatca
     2581 ggaagtacat cggcaccagc atcatttccc agaagaagaa gaacaggaac atgtcgatgg
     2641 caaggaacac gccgataacg ccgcccagga tccacatcag gttgaggtgg aagaagccct
     2701 gatatttttc gatctctttc cacgaacaga gtaccgccag cacaccgagc agaccggtca
     2761 gcacgaccat cagcagcgac agcccgtcaa tggcgagatg gatagagata ccaaaacgcg
     2821 ggatccacgg catgtcgaat tcagactgcc actgcggaat tccggcggat tgcgtcagtg
     2881 aataaccgcc ctgcaaccac agttgcagcg acagcgccag cgtcaatccc atggtgatca
     2941 gcgcgatcca gcgcggcacc ttgacgccaa agcgttcggt ctgccagcac aggaagccgc
     3001 cgataaaggg aattaatatt agccagggta gtaacatggc gatctttatt ccttgtaaaa
     3061 gtcccgtcag gaccggattt tcaacgaatt ctcacgacag aaaattctct tcggactggg
     3121 ggtagtgtcg gatgcaccgc gaatgcggct tatccgacct acgttcttat atgcccaggg
     3181 gcaatcccac aatccttaac tcaacgcagt accatcaaca gtgccagcac cacgaccgca
     3241 ccgatgctca tggatgccac ataccagcgc agataaccgt tctcgcttaa cagcagacct
     3301 ttacctgcaa agcgggaaag gacagccggg atgttcatca ttgagttcag cggatcgcgt
     3361 ttcagcaacc aggcaatacc caggaacggc ttgacgaaca ctttgtcgta cagccagtca
     3421 aagccccagg cgttgtacca ccaggtaccc agcagacggc ccggcgcact gttggcgatg
     3481 gaggtcacca gagtacgttt acccagccac agccaggcag ccagcagaat gccgaccacc
     3541 gcgaccacgc cagaggtaat ttccagggtc aacatgctgc cgtgcgccag ttccgtcgtt
     3601 tgcggaagca cgccctgcag cggcggtaca atcagtgcgc caacgaaggt ggaaaggatc
     3661 agcagcacaa tcagcggcag gctgtgagtt acccctttca cggcgtgagc gtgaatttgt
     3721 tcttttccgt ggaagacgat gaaaatcata cggaaggtgt agagcgaggt cataaacgca
     3781 ccgaccagac ctgccaccat cagattgata tgaccattcg ccatcgcacc cgcgaggatc
     3841 tcatccttac tgaagaagcc cgcagtgacc agcggcagtg ccgacagtgc tgcgccgccc
     3901 accaggaagc agagataaac cagcggaata gatttacgca gaccgcccat cttgaagatg
     3961 ttctgttcgt gatggcaggc cagaatgacg gaaccggatg ccaggaacag cagcgcttta
     4021 aagaacgcgt gggtcatcaa gtggaaaatc gccgcatccc atgcctgcac gccaagcgcg
     4081 aggaacatgt agccaatctg gctcatggta gagtaagcga gaacacgttt gatatcggtc
     4141 tgtaccagcg cggcaaaacc ggccagcagc agcgtaaccg ccccgacaat gcccaccaga
     4201 tgcagaactt ccggcgtcat caggaacagg ccgtgggtac gagcgatcag gtagacaccc
     4261 gcggttacca tcgttgcggc gtggatcagc gcggagacag gcgtcgggcc cgccatcgcg
     4321 tcggcaagcc atgtctgcaa cggcaactgc gcagatttac cgaccgcacc gcccagcagc
     4381 atcagcgtcg cccacatcag catgttattg ccgtcagcaa agtgcgctgg tgccagttcc
     4441 accatttcgc ggaagttcag ggtgcccagt tcgttgtaaa gaatgaacag cgcgaaagcg
     4501 aggaacacgt cacccacacg ggtcacgacg aacgctttca ttgccgctgc gccattcttc
     4561 ggatcggtgt aatagaaacc gatcagcaga taggagcaca ggcccacgcc ttcccagccg
     4621 aggtacatca acagtaggtt atcggcgaga accagaacta ccatgctggc gatgaacagg
     4681 ttggtgtaag cgaagaagcg agagtagccc tcttctccgc gcatatacca ggaggcatac
     4741 atgtgaataa ggaaacccac accagtgacc actgagagca tggtcagcga caggccgtcc
     4801 agcaccagat tgaaaccaat gttaaagtcg cctaccgaca tccacgtcca cagcggctgg
     4861 ctgtatgcct gctcaccgtt agcgaagaag tcaacgccga taaaggcggt taccagcgcc
     4921 gccaggccca cagagccaac gccgacgatc gccgagacgt tttcagacca gcgcccacgg
     4981 gagaacgcca gcaggacgaa gccaatcaat ggcaaaataa tggttaaggc aagcatgttc
     5041 atccgcgcat ctcacttact gaatcgatgt tcaggttctg gcgacgacgg tgaagttgca
     5101 gcagcagcgc aaggccgata ctcgcttccg ccgccgcgag gctgatggcg agaatgtaca
     5161 tcacctgacc gtcggtctgg ccccagtagc ttccggcgac cacaaaggcc agcgcagagg
     5221 cgttaatcat gatttccaga ccaatcagca taaacagcag attgcgacgg ataaccagac
     5281 cggttaagcc aagaacgaat aagattgccg cgaggatcag tccatgttgt aaggggatca
     5341 tgcgtgctcc tccgtttttc ttttcgcgct gtcgtcttta cgattgctca gcacttcacc
     5401 cgcacgctct tcacgaccga cgtggaaggc cacaaccaga cccgcgagca gcagcataga
     5461 agccagttcc accgccagta cgtaaggccc gaacagcgta atacccactg ctttagcact
     5521 gattggcgta ccgtcgatac cctgatcatt aacaccaagg atggcgtaaa caatcaccac
     5581 cagcatgatg gccgacaaaa tcgccggacc aatccacacc tgcggtttca gccactggcg
     5641 ttcctgttcg atttctgaac cgcccaggtt gagcatcatc accacgaaca cgaacagcac
     5701 cataatggca cccgcgtaga cgataatttc cagcgcaccg gcgaagtaag cgcccagtga
     5761 gaagaacacc ccagaaatcg ccagcagcga aataatcagg tacagcagcg cgtgtaccgg
     5821 attggtatgg gtgatcactc gcaaggtcgc aagtatggct atcaggccac agatataaaa
     5881 agcgaactcc attgcccctc tccttacggt aacaggctct tgacgtcgat aggcttggct
     5941 tcgttctctg cttcgccctt atctttgccg tcgattgcca tacctgccat ccggtagaag
     6001 ttatattccg ggtatttgcc cggaccggag atcagcagat cctctttctc gtaaaccaga
     6061 tcctggcgct tgtattcccc catttcgaaa tccggggtta actgaatcgc cgtggtcgga
     6121 caggcttctt cgcacagacc acagaaaatg cagcgtgaga agttgatgcg gaaaaattcc
     6181 ggataccagc gaccgtcttt ggtttctgct ttttgcagcg agatacagcc gaccgggcag
     6241 gctaccgcgc agaggttaca ggctacgcaa cgctcttcgc cgtccggatc gcgggtcaga
     6301 acgatacgac cacgataacg gggcggcaga tagaccggct cttccgggta cattcgcgtt
     6361 tcgcgtttgg cgaacgcgtg caggccgatc atccagatac tacgaacctg ggtgccgaaa
     6421 cctactaaca attcttttaa ggtcatggtc ttattgcccc ttattgcgcc tgccagagaa
     6481 tgacagccgc cgttaccagc aagttgatca gcgtcagcgg caggcagatt ttccagccga
     6541 aggacattac ctggtcataa cgcggacgcg gtaacgacgc acgaatcaaa atgaacatca
     6601 tcataaagaa cgcggttttc agcgcgaacc agatgaatgg cggtaacaac gggccttgcc
     6661 agccaccgaa gaacagcgtc accatcagtg cagagatggt cacaatcccg atgtattcgc
     6721 ccacgaagaa cagaccgaac ttcatgccgg aatattcaat gtggtaacca tccgccagtt
     6781 cctgctcggc ttccggctgg tcaaacgggt gacggtgaca caccgccacg cccgcgatgg
     6841 caaaggtaat aaaaccaaag aattgcggga tgacgttcca cacatgcgcc tggctgttga
     6901 cgatgtcggt catgttgaat gaaccggcct gcgccaccac gcccatcagg gaaagcccga
     6961 ggaacacttc gtagctcagg gtctgcgcag aagcacgcat cgcacccagc agcgagtatt
     7021 tgttgttact tgaccagccc gcaaacagca ccgcgtaaac cgccagaccg gccatcatca
     7081 ggaagaacaa aatcccgatg ttcaggtcgg caaccaccca acccggactg actggcacaa
     7141 tcgcaaaggc cagcagcagc gaggtaaagg caatcatcgg tgccagggta aagatgacgc
     7201 gatccgagaa tttcgggatc cagtcttctt taaagaacat cttgatcatg tccgcgacca
     7261 gctggagcga accgccccag ccaacacggt taggtccgta acggttctgg aacagaccca
     7321 gcaggcgacg ttcgccaaag ctcatgaatg ccccacaggt gacaaccacc agcaggatca
     7381 ccaccgcttt gaggatggtc agcaggatct caatcagttc cggtgatatc caactcattg
     7441 ttgtgcctcc ttgagatcct caagatgcgc gccagccagc accggagcaa tgccggacat
     7501 acccatcggc aagcccacct gccctgccgt cagtccttcg gcgatttcaa ccggcagcgt
     7561 gaccgtgttg ccatcgtaac taaaggagac gcgtgtaccc gcgttcacac ccaacttcgc
     7621 ggcatccgct gggttgagtt tgatgtacgg ctgcggcata cggctctgga agaccggagc
     7681 acgctgtgac aattcatcgc taccaaacag gtggtagtac ggcgcgatac gccatttccc
     7741 gtcctgcggc tggaagcgtg ccggtacgct ggtgaagtaa tccagaccat tttcgctggt
     7801 ttcaaacaga cgcacgcccg gatcgccaaa gcgcagtttg ccgcccactt cgtcctggaa
     7861 tttgttccac gcctgcgggg agttccagcc tggcgcccag gcaaacggca cttgcgaacg
     7921 gtgcgcggtc ggctggttgt taccttccat cgagaaggtg aacatggtgt caatatcctg
     7981 cggctgacgc ggctcatgaa cgctgatatt ggcgcgcatg gcggtacgac cgctgtaacg
     8041 gtgcggttca cgggccagtt tctgcccacg aatacggaat gtcgcatccg gcgcagcatc
     8101 tttgataccc gccagttccg ggattttcgc cacaacagcg tcaatcacat ggtcgagctg
     8161 cgtccagtcc acttcacggc tcagcaaggt gctgtgcagc gagtgtaacc aacgccagct
     8221 ttccagcatg acagttttgc tgtcgtaata agccggatcg taaacctgga agaaacgttg
     8281 ggcacggcct tcgttgttaa tcaccgtacc gtcgctttca gcaaagctgg cagcagaaag
     8341 taccagatgg gcgttttcca taatcgctgt gcgttgatgg tcaaccacca tcaccagcgg
     8401 cgctttagcc agcgcagcat tcacgcgggt agcggaagcg tgacgatgca gatcgttttc
     8461 cagcaccacc accgcgtcgg cgcgtccggt ttccagttcg gttaacgctt cttcaagcga
     8521 tccaccgccc ataatgccca gccccatgct gttgacggaa cgggcaatca tggtgatacc
     8581 gacgtcagcg ccgcgacctt tcagggcttt cgccacgtta gccgcagcct gaatcacttc
     8641 agcgctaccg gcgttcgtcc cggagataat caacggtttc ttcgcacctg ccagtgcctg
     8701 cacgatgacg tcgattttgc tttgcagctc aggttcaata ccgtcaaccg ctggcgcgga
     8761 gttatccagc gcatgggcga tggcaaaacc taaacgcgcc tgatcttcaa ccggtgcgcg
     8821 gtaagtccac gccgcgatat catccagacg ggtgtcatca acgttggtaa caaacagcgg
     8881 atgcttcgca cgttggccga tgttgaggat tgccgcaatc tgccagtcag ccactttctg
     8941 tgctgccgcc atttcgcgcg ctttaccttt caccgcctga cgaactgcca gcgcgacgcg
     9001 cgcgccggtc tgggtaacgt cttcgcccag caccagtacc gcatcgtaag attcgatttc
     9061 gcgcagtgcc ggcgtataaa tgccgccttc acgcagcact ttcagcgcca gttgcagacg
     9121 ttcctgctca ccgtgagcga taccggtgta gaagttttct tcgcccacca gctcacgcag
     9181 cgcaaagttg ctttccacgc tggcacgcgg agaaccaatg ccgatcactt tcttcgactg
     9241 acgcagaata tctgccgcgc cctgcattgc ctgttcggcg ttgagggtaa tgaaatcatc
     9301 gccacgacgc tgtactggct gacgcggacg atctttcagg ttgacgtaac cgtaaccgaa
     9361 acgaccacgg tcgcagagga agtagtggtt taccgtaccg ttgtaacggt tttcgatacg
     9421 acgcagttcg ccgtaacgct cgccggggct gatgttacag ccgatggaac attgctggca
     9481 gatgctcggc gcaaactgca tatcccattt acggttgtaa cgctcggagt gcgttttgtc
     9541 ggtgaatacg ccggtcgggc aaatctcgac cagattaccg gaaaattcgc tttccagcgt
     9601 gccgtcttcc gggcgaccga agtagacgtt gtcgtgcgca ccgtaaacgc ccaggtccgt
     9661 gccgtcagcg taatctttgt agtaacgcac gcagcggtag caggcgatgc agcggttcat
     9721 ttcgtgagag atgaatggcc ccaaatcctg attacggtgg gtacgtttgg tgaaacggta
     9781 gcgacggaag ctgtgtccag tcatcacagt catatcctga agatggcagt taccgccctc
     9841 ttcacatacc ggacagtcgt gcgggtggtt ggtcattaac cactcgacca cactttcacg
     9901 gaactgtttc gcttcttcat cgtcaatgga aataaaggtg ccatcggaag ccggtgtcat
     9961 acaggacatc accaggcgac cacgcgtgtc ttccgcgttt tggtattgct tcaccgcaca
    10021 ctggcggcaa gcaccgacgc ttcccagcgc cggatgccag caaaagtaag gaatatcaag
    10081 gcccagagac agacaagctt ccagcaagtt gtccgctccg ttgacctcgt attctttgcc
    10141 gtctacatga attgtagcca ttagcatgct tccagttttc tcagtcagag actgagcgtt
    10201 aatcgaaatt cggttaccag cgctctttca gcaggttcgg ctgaatccca ttaatcaaat
    10261 gggtattgct gaacggctgt ttgattcccg cctcaaattc ttcgcggaaa tatttgatgg
    10321 cgctctgtaa cggctccact gcaccaggtg cgtgggcaca gaaagtttta cccgggccta
    10381 agaatcgaca cagttgctca agtgtttcga tatcgcccgg ctgaccttcg ccacgctcca
    10441 gcgcacgcag aattttcacg ctccacggca gaccgtcgcg gcacggcgta caccagccac
    10501 aggactcacg ggcgaaaaac tcttccaggt tacgcaccag cgacaccatg ttgatctcat
    10561 ggtcaaccgc catcgccagc gccgtaccca gacggcttcc cgctttaccg atactttcga
    10621 attccatcgg cagatcaagg tgcgcttcgg tcaggaagtc ggtgccagcg ccgcctggct
    10681 gccaggcttt gaatttcaga ccgtcacgca taccaccggc gtaatcttcg aggatctcgc
    10741 gtgcggtggt gccgaatggc agttcccaca ggcccggatt tttcacccga ccggagaagc
    10801 ccatcagctt ggtgccagca tctttacttt tcgagatgtt ctgataccac tccacgccgt
    10861 tagcgaggat cgccggaacg ttacacaggg tttcgacgtt attgacacag gtcggtttgc
    10921 cccatgcacc ggaggttgcc gggaatggag gcttcgagcg agggttagcg cgacgccctt
    10981 ccagggagtt gattaacgct gtttcttcac cgcagatgta gcgcccagcc ccggtatgga
    11041 cgaacaattc gaagtcgaaa cctgttccca taatgttttt gccaagcaga cccacttcgg
    11101 tggcttcggc aatggcacgg cgcagattca ctgccgcttc gatatactcg ccacgcagga
    11161 agatgtagcc acggtacgct ttcagcgcaa acgcggagat gagcatacct tccaccagca
    11221 ggtgcggcag ttgttccatc agcaggcggt ctttataggt gcccggctcc atttcatcgg
    11281 cgttacacag caggtaacgg atgttcatgg attcgtcttt cggcatcagg ctccatttca
    11341 ggccggtaga aaagcccgcg ccgccgcgcc ctttcagacc agcgtctttt acctgattaa
    11401 cgatttcgtc cggagacagc ccggtcagcg ccttacgcgc gccttcgtaa ccgtttttgc
    11461 tgcgatattc gtccagccac actggctgtt tgtcatcgcg cagacgccag gtcagcggat
    11521 gcgtttcggg agtacggata atgtttttca tttataccgc tccagcagtt cagggatcgc
    11581 ttccggggtc agatgcgcgt gagtgtcctc atcgatcatc atgtttggcc ctttgtcgca
    11641 gttccccagg cagcaagttg gcagcagcgt aaagcggcca tcaaatgtcg tttgccctgg
    11701 tttgatgttc agctttttct ccagcgccgc ctgaataccc tggtaaccgt tgatgtgaca
    11761 gaccacgctg tcgcaataac ggatcacatg gcgaccaacc ggctggcgga agatctggct
    11821 atagaacgtt gccacgcctt cgacgtcgct cgccggaata cccagcacat cggcgatcgc
    11881 gtggatcgcg ccatccggca cccagccacg ctgcttctga acgattttca gcgcttcaat
    11941 ggacgccgca cgcgggtctt cgtagtggtg catctcgtgc tcaatcgctt cacgctctgc
    12001 cgcactcagc tcaaaagcct cggtttgtgg ttgttgattc tcgtgcataa ttagcggtcc
    12061 acatctgaca taacaaaatc gatactgccc agataaacaa tcaggtcaga caccaggctg
    12121 ccgcggatcg ccgccggaat ttgctgcaaa tgcgcataac tcggcgtacg gatacgggtg
    12181 cgataactca tggtgctacc gtcgctggtc aggtagtaac tgttgatccc cttggtcgcc
    12241 tcaatcatct ggaaagattc attggctggc atcaccggac cccacgacac ttgcaggaag
    12301 tgggtgatca gggtttcgat atgttgcagc gtgcgctctt tcggcggcgg cgtggtcagc
    12361 ggatgatccg ctttgaacgg gccttccggc atgttgttga ggcactgctc aagaatgcgc
    12421 agactctggc gcagctcttc cactttcagc attacgcggg tgtagcagtc agaaacacca
    12481 ccacccaccg ggatttcaaa gtcgaagttt tcatagccag aataaggacg cgccttacgc
    12541 acgtcgaagt cgatcccggt ggcgcgcagg cccgcgccag tggtgcccca ctccagcgcc
    12601 tctttcgcgc cataggcggc aacgccctgg gaacgacctt tcagaatggt gttttgcagc
    12661 gccgctttct cgtaagacgc cagacgtttc ggcatccagt cgaggaactc acgcagcagg
    12721 cgatcccagc cacgcggcag gtcgtgcgct acaccgccaa tacggaacca cgccgggtgc
    12781 atacgaaaac ccgtgatcgc ttccaccaga tcgtaaattt tctgacgatc ggtaaaggcg
    12841 aagaacactg gcgtcatcgc gccgacgtcc tgaataaagg tggagatata cagcaggtga
    12901 ctgttgatgc ggaacagttc ggagagcatt acgcgaatga cgttaacgcg atccggcacg
    12961 gtgatcccgg ccagtttctc taccgccagc acgtaaggca tttcgttaac gcagccgccg
    13021 aggtattcga tacggtcggt atacggaatg tagctgtgcc aggactggcg ttcgcccatt
    13081 ttctccgcac cacggtggtg gtagccgatg tctggtacgc agtcgacaat ttcttcgcca
    13141 tcaagctgca aaacgatgcg gaaagccccg tgcgccgacg ggtggttcgg gccgaggttg
    13201 aggaacatga agtcctcgtt ttcggtgccg cgcttcatcc cccactcttc cggtttgaag
    13261 gtcagggctt ccatctccag gtcctgtttg gctttggtca gctcaaacgg cgagaattcg
    13321 gtagcgcgcg ccggataatc tttacgcagc gggtgacctt tccaggtttg cggcatcatg
    13381 atgcggcgca ggttcgggtg accgtcgaaa gtaatgccaa acagatccca ggtttcacgc
    13441 tcataccagt tagcgttcgg gaacagtttg gtgaaggtcg gtacgtgcag gtcgttttct
    13501 gccagcgcca ccttcagcat gatgtcgcgg ttacgatcga tagaaatcag atggtagaaa
    13561 acggaaaaat ccgcggcagg taacccttcg cggtgtgtgc gcagacgttc gtccatgccg
    13621 tgtaagtcaa acagcatgac gtaaggtttc ggcagtttct ttaagaaatc gcccacttcc
    13681 agtaattgtt cacgcttgat ccacacaacg ggaaccccgg tgcgagtcgc ctgaacagta
    13741 aaggcatccg gcccaaaacg gttgcgcagt tcgccaatca ccggatcatc aagatgatcg
    13801 cgggtctgcc aggcgggttc ttgcgcggtt aagtcggtca tattgttcac cattgcaaat
    13861 ggtccgtggt gactgtcggc gcagtaagct tcgcgaaata gatttaggaa taagcgaaga
    13921 aaaattcctt tgccgacagg cgcaaattaa atctcgtcag gtgtacgcag gttggttacg
    13981 gcaatgcgtt caccgcgctt gcgttcgcgc tctgattgca tattggcgcg ataaacgccc
    14041 tgatcgccaa ccacccagga gagcggacga cgttctttac cgatagattc ctgcaacagc
    14101 atcagtgcct gcatgtacgc ttcaggacgc ggagggcagc ccgggatata cacatcaacc
    14161 gggatgaatt tatcgacgcc ctgcacaacg gaataaatat cgtacatacc gccggagttg
    14221 gcacaggcac ccattgagat aacccatttt ggttccagca tctggtcata cagacgctga
    14281 ataaccggtg ccatttttgt aaagcaggtt cctgcaacga ccatcaggtc agcctgacgc
    14341 ggcgaagctc gcaatacttc tgcgccaaaa cgcgccacgt catgcaccgc ggtaaacgaa
    14401 gtcaccatct caacgtaaca gcaggaaaga ccgaagttat acggccaaat tgagttttta
    14461 cgaccccagt taaccatgtc attgagcttg cccataaaca cgtttttatt aacttcttgc
    14521 tccagagggt cggttacgat ctcctgcttt tgcagggggt aacggtcgtt ctcaccgttg
    14581 ggatctatgc gggtgagcgt ataatccatc ttaatgcctc gcggttagcg ttgacgatta
    14641 gcgatactgt tcgtttccgg gttcatacgc tcgcggcgtg aacgcgcggg cgtccagtcc
    14701 agcgcgccaa tacgcaccag ataaaccaga cctgccagta acacaaaaat aaaaattgca
    14761 gcttccacaa agcctaccca gccgctttcg cggatagagg ttgaccatgc gaacagatac
    14821 agcgcttcaa cgtcgaagat aacgaagaac atggccacca gataaaactt ggcggacagg
    14881 cgtaagcggg cggagccgac cgagtcgata ccggattcaa acggaacgtt tttcgacctt
    14941 gcgcgtgcgc gaccgcctaa aaaccaaccg cctaccagca tcaggcaaca caggccaatg
    15001 gcaacgataa gaaagatagc gaatgcccag tgatgagcga tgacttcagt ggatgttgac
    15061 atactcattg cttactcatc aaaagtagcg ccagattcac tgctcttcac ggcagatgga
    15121 cgccacatcg attcatgggg aggaataaaa aaaaccttac aatcactgta gaaattcttt
    15181 tatacagcta attgatgtgg tcttttactc ctttctataa ccttttgtca actttaacaa
    15241 aagtttcttc acattagttt acataatatc aacaccatta gcatttaatg ccctttcacc
    15301 ccagatcctt gacgactcca ggataattag atgttgttga atcgtgtccg ttgtgaagca
    15361 atggaaaaaa tacgggtcta ttttcacagg aatttgtgtc gattcctccc cccaaaagag
    15421 agtattttct tgatctgtga cacgcttttg tcattccata acaaaaacgc agcaacaaat
    15481 ttacgtattt tttaacatca ttgtagcagg tgattttttt caggcgatta tttgtgcgtt
    15541 cgggacgtga atctctggtg attgaaaaat gaacagtttt gtacgttctg cacgatacga
    15601 ggaaggcttt ttactaaaaa aaagccgctg gggtttaaaa cacccccagc ggctcgtttt
    15661 tacactattg tctcagaaat tatctatggt ccgtcgatta ctcgatatcc ctttcaatca
    15721 acaatgagtc atcgccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc
    15781 tctccatcgc ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca
    15841 gcaaatattc ggtatcgggt agcggcggta agccatctac tccactcagc acacgcaggt
    15901 ctggactcat catctcgacc ggccttgccg tcacgccaag accggctttt actgccgcac
    15961 gaactgccgg aagcgtcgag gcgacataag ccagtcgcca tggaatatct gctttattaa
    16021 gcgtcgccag caccatatcg cgaaacgggc taggatcatc cagcaataca agagggatcg
    16081 gctcgccttt ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca
    16141 ggttcaatgc cttaaacgtt gaaggacgat gggtggttac catcaaatcc acttcctggg
    16201 attccagcat ttctgccata taggcattac gtttaacgcg gacatccagc gctagtttcg
    16261 gataaaccga actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg
    16321 aagcaccgat agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat
    16381 cattaaaacg cagaattttt ctggcgtaac caagaagttg aatgccatgt tcagttaaca
    16441 gtttgttgcg accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca
    16501 tttgctgact tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt
    16561 tcagatcggc aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac
    16621 gatttgcact tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg
    16681 tgcacacaca aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac
    16741 ccggacgaaa gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt
    16801 ataatgcctg aagtagatca cagaatatat cttcaggaat cgcatatcta ttaagttact
    16861 cactcttttg tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc
    16921 cactctcgtg cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaggcgg
    16981 catcaagagc agggatccgt catttatgcg agcattttac cccaaaaacc ttttatttat
    17041 aagggtcatt gcgaa
//
LOCUS       DAZUCC010000106        16834 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZUCC010000106 DAZUCC010000000
VERSION     DAZUCC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.106"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(25..720)
                     /gene="araD"
                     /locus_tag="ACHY09_002901"
     CDS             complement(25..720)
                     /gene="araD"
                     /locus_tag="ACHY09_002901"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS3885853.1"
                     /translation="MLEDLKRQVLEANLALPKHNLITLTWGNVSAVDRERGVLVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(920..2422)
                     /gene="araA"
                     /locus_tag="ACHY09_002902"
     CDS             complement(920..2422)
                     /gene="araA"
                     /locus_tag="ACHY09_002902"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS3885854.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGIKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(2433..4133)
                     /gene="araB"
                     /locus_tag="ACHY09_002903"
     CDS             complement(2433..4133)
                     /gene="araB"
                     /locus_tag="ACHY09_002903"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFS3885855.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIANKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            4472..5350
                     /gene="araC"
                     /locus_tag="ACHY09_002904"
     CDS             4472..5350
                     /gene="araC"
                     /locus_tag="ACHY09_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFS3885856.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAAKLS"
     gene            5436..6200
                     /gene="yabI"
                     /locus_tag="ACHY09_002905"
     CDS             5436..6200
                     /gene="yabI"
                     /locus_tag="ACHY09_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFS3885857.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLAPLISAVGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6314..7012)
                     /gene="thiQ"
                     /locus_tag="ACHY09_002906"
     CDS             complement(6314..7012)
                     /gene="thiQ"
                     /locus_tag="ACHY09_002906"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFS3885858.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERSEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASAILGITG"
     gene            complement(6996..8606)
                     /gene="thiP"
                     /locus_tag="ACHY09_002907"
     CDS             complement(6996..8606)
                     /gene="thiP"
                     /locus_tag="ACHY09_002907"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFS3885859.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(8582..9565)
                     /gene="thiB"
                     /locus_tag="ACHY09_002908"
     CDS             complement(8582..9565)
                     /gene="thiB"
                     /locus_tag="ACHY09_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFS3885860.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9596..9695)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9729..11384)
                     /gene="sgrR"
                     /locus_tag="ACHY09_002909"
     CDS             complement(9729..11384)
                     /gene="sgrR"
                     /locus_tag="ACHY09_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFS3885861.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAA
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQILPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11473..11604
                     /gene="sgrT"
                     /locus_tag="ACHY09_002910"
     CDS             11473..11604
                     /gene="sgrT"
                     /locus_tag="ACHY09_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFS3885862.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11712..12317)
                     /gene="leuD"
                     /locus_tag="ACHY09_002911"
     CDS             complement(11712..12317)
                     /gene="leuD"
                     /locus_tag="ACHY09_002911"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFS3885863.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12328..13728)
                     /gene="leuC"
                     /locus_tag="ACHY09_002912"
     CDS             complement(12328..13728)
                     /gene="leuC"
                     /locus_tag="ACHY09_002912"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFS3885864.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13731..14822)
                     /gene="leuB"
                     /locus_tag="ACHY09_002913"
     CDS             complement(13731..14822)
                     /gene="leuB"
                     /locus_tag="ACHY09_002913"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFS3885865.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            complement(14822..16393)
                     /gene="leuA"
                     /locus_tag="ACHY09_002914"
     CDS             complement(14822..16393)
                     /gene="leuA"
                     /locus_tag="ACHY09_002914"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFS3885866.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16486..16572)
                     /gene="leuL"
                     /locus_tag="ACHY09_002915"
     CDS             complement(16486..16572)
                     /gene="leuL"
                     /locus_tag="ACHY09_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFS3885867.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRLVSGIQN"
BASE COUNT         3713 a         4584 c         4584 g         3953 t
ORIGIN      
        1 attggctgtg gttttataca gtcattactg cccgtaatat gccttcgcac catgcttacg
       61 cagatagtgt ttatccagca gcgtttgctg catatccggt aactgcggcg ctaactgacg
      121 gcagaatatc cccatataag cgacctcttc cagcacgatg gcgttatgca ccgcatcttc
      181 ggcatttttg ccccatgcaa atgggccgtg agaatggacc aggacgccgg gcatttgtgc
      241 tgcgtcgatt ccctgttttt cgaaggtttc tacgatgacg ttaccggttt cccactcata
      301 ttcaccgttg atttctgcgt cggtcatttt gcgggtgcag ggaatggtgc cgtagaaata
      361 gtcggcgtgg gtggtaccgg ttgccggaat tgactggccc gcctgcgccc agatggtggc
      421 gtggcgcgag tgtgtgtgca caatgccgcc aatggacggg aatgcctgat agagcagccg
      481 gtgagttggc gtgtcggagg agggcttttt cgttccttca accacttcac cggtttcgat
      541 gctgaccacg accatatcgt cagcggtcat gacgttgtaa tcgacgccgg aaggtttgat
      601 caccaagacg ccgcgctcgc gatcaacggc gctgacattg ccccatgtga gcgtgatcag
      661 gttatgtttt ggcagcgcca aattggcttc taatacctgg cgtttgagat cttctaacat
      721 gttgactcct tcgtgccgga tgcgctttgc ttatccggcc tacaaaatcg cagcgtgtag
      781 gcctgataag acgcgtcagc gtcgcatcag gcggtgaatg ccggatgcgc tttgcttatc
      841 cggcctacaa aatcacagcg tgtaggcctg ataagacgcg tcagcgtcgc atcaggcgtt
      901 acacacctga tgcggctact tagcgacgaa acccgtaata cacttcgttc cagcgcagcg
      961 cgtctttaaa cgctggcagg cgggtatcgt tatcaatcac cgtgatttca atgtcgtgca
     1021 tctcggcgaa ctggcgcata tcgttgaggt tcagcgcatg gctgaaaacg gtatggtgcg
     1081 cgccaccagc aaggatccac gcttcggaag cagttggcag atccggttgc gctttccaca
     1141 gcgcattcgc caccggcagt ttcggcaggg agtgcggtgt tttcaccgtg tcgatgcagt
     1201 taaccagcag acggtaacga tcgccgagat caatcaggct ggcgacaatc gctggaccgg
     1261 tttgggtatt gaagatcagg cgggcaggat cgtctttacc gccaataccg aggtgctgaa
     1321 cgtcgaggat cggtttctct tccacagcga tcgacggaca gacttccagc atgtgggagc
     1381 cgagcaccag gtcattgcct ttctcgaagt gataggtgta gtcctccata aaggaggtgc
     1441 cgccctgcag accggttgac atcaccttca tgatgcgaag cagggcggca gttttccagt
     1501 cgccttcgcc cgcaaagccg taaccctgct gcatcagacg ctgtacggcc agaccaggaa
     1561 gctgtttcag accgtgcaaa tcttcaaagg tggtggtgaa cgcgtggaag ccaccttgtt
     1621 ccaggaaacg cttaatcccc agctcaatac gcgccgcttc cagcacgttc tggcgttttt
     1681 cgccgtggat ttgtgtggca ggcgtcatgg tgtagcagct ttcgtactca tcgaccagcg
     1741 cgttaacatc gccatcgctg atggagttca ctacctgcac cagatcgcca accgcccagg
     1801 tattgacgga gaaaccgaac ttgatctgtg cggcaacttt atcaccatcg gtgaccgcca
     1861 cttcacgcat gttatcgcca aaacggcaga ctttcagatg acgggtatcc tgtttagaga
     1921 ccgcctgacg catccaggag ccgatacgct catgggcttg tttatcctgc cagtgaccgg
     1981 taacgacggc atgttgctga cgcatacgcg cgccaatgaa gccgaactcg cgaccgccat
     2041 gtgcagtctg gttcaggttc ataaagtcca tatcgatgct atcccacggc agcgccgcgt
     2101 taaactgggt gtggaattgc agcaacggtt tgttgagcat agtcaggccg ttgatccaca
     2161 ttttggccgg ggagaaagtg tgcagccaca ctaccaggcc agcgcaacga tcgtcgtaat
     2221 tcgcgtcacg gcaaatagcg gtgatttcat ccggcgtggt gcccagcggt ttcagcacca
     2281 gtttgcaggg cagtttcgct tccgtattca gcgcattaac gacgtgctcg gcatgttggg
     2341 tgacctgacg cagggtttcc gggccataca gatgctggct gccaattaca aaccacactt
     2401 cataattatc aaaaatcgtc attgtcgtgt ccttatagag tcggaacggc ctgggcagcc
     2461 tgtgccgggg cggaagttgg aagatagtgt tgttcggcgc tcatcgccca ttgctgatag
     2521 cggcgataaa gctgttcaaa gcgttgtgcc tgttcgctgc gcggttgcag ggttttctct
     2581 accgcactgg ccattttttg ctgggctgag gggatgtctg cgtgcacttt cgcggcgacg
     2641 gcagcgaaaa tcgccgcacc gagcgcacag cactggtcag aggcaacaat ttgcagcggg
     2701 cgattcagca cgtcgcagca ggcctgcata atgacctggt ttttccgcgc gatgccgccc
     2761 agcgccatca cgttattgac ggcgatcccc tgatcggtaa agcactccat gattgcgcgt
     2821 gcgccaaagg cggtggcagc aatcaatccg ccgaacagca gcggagcgtc ggtagcgagg
     2881 ttcagatcgg taatcacccc tttcaggcgt tggttagcgt ttggcgtgcg gcggccgtta
     2941 aaccagtcga gcaccaccgg caggtgatcc agagacggat ttttggccca tgcttcggtc
     3001 agcgccggaa gcagttgttt ctggctggcg ttgatttgct ctttcagttc cggatgctgg
     3061 gcggcaagct gttccagcgg ccagccgagt acgcgaccga accaggcgta gatatcacca
     3121 aacgccgatt ggcctgcttc cagaccgata aatccaggca ccacgctgcc atcaacctga
     3181 ccgcaaatac ctttcactgc ccgctcgcca acgctctgtt tgttggcaat cagaatgtcg
     3241 caggtggaag taccgataac ttttaccaga gcgttaggct gtgcgcctgc gccaactgcg
     3301 cccatatggc agtcaaacgc gccgccggaa atcaccacgc tttcaggtag gccgagacgc
     3361 tgcgcccatt ccgggcataa ggtgcccacc ggaatatcgg cagtccaggt gtcagtgaat
     3421 agcggggaag gcaaatggcg attgaggatc gggtccagct catcaaagaa actggctggc
     3481 ggcaggccgc cccagctttc atgccatagc gacttatgcc cggcgctgca acgtccgcga
     3541 cgaatatcct gcgggcgggt ggtaccggaa agcagagctg gcacccagtc gcacagctca
     3601 atccacgatg cggcagattg cgccacggcg ctgtcctggc gagtcacatg cagtattttt
     3661 gcccagaacc attcgctgga ataaataccg ccaatatagc gggagtagtc aacgttgccc
     3721 ggcgtgtgac ataagcgggt tatctcttcc gcttcttcaa ccgcggtgtg gtctttccac
     3781 aatacgaaca tcgcgttcgg gttttcggca aactccgggc gcagcgccag gacattaccg
     3841 tccgcgtcaa tcggtgcggg cgtcgagccg gttgtgtcaa cgccaatccc gaccacagct
     3901 gcgcgctgct cggcgctaag ctctgcaagc acggttttca gcgccgcttc cattgactca
     3961 atgtagtcac gcggatgatg acggaactgg ttatttgggg catcacaaaa ttgccctttc
     4021 tgccaacggg gataccactc tacgctggtg gcgatctctt caccggtggc gcagtccacc
     4081 gccaaagctc gcacagaatc actgccaaaa tcgaggccaa ttgcaatcgc catcgtttca
     4141 ctccatccaa aaaaacgggt atggagaaac agtagagagt tgcgaaaaaa agcgtcaggt
     4201 aggatccgct aatcttatgg ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa
     4261 ataatcaatg tggacttttc tgccgtgatt atagacactt ttgttacgcg tttttgtcat
     4321 ggctttggtc ccgctttgtt acagaatgct tttaataagc ggggttaccg gttggattag
     4381 cgagaagagc cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg gacaattggt
     4441 ttcttctctg aatggcggga gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct
     4501 gccgggatac tcgtttaacg cccatctggt ggcgggttta acgccgattg aggccaacgg
     4561 ttatctcgat ttttttatcg accgaccact gggaatgaag ggttatattc tcaatctcac
     4621 catacgcggc cagggggtgg tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga
     4681 tattttgctg ttcccgccag gagagattca tcactacggt cgtcatccgg aggctcgcga
     4741 atggtatcac cagtgggttt actttcgtcc gcgcgcctac tggcatgaat ggcttaactg
     4801 gccgtcaata tttgccaata cgggtttctt tcgcccggat gaagcgcacc agccgcattt
     4861 cagcgacctg tttgggcaaa tcattaacgc cgggcaaggg gaagggcgct attcggagct
     4921 gctggcgata aatctgcttg agcaattgtt actgcggcgc atggaagcga ttaacgagtc
     4981 gctccatcca ccgatggata atcgggtacg cgaggcttgt cagtacatca gcgatcacct
     5041 ggcagacagc aattttgata tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg
     5101 tctgtcgcat ctgttccgcc agcagttagg aattagcgtc ttaagctggc gcgaggacca
     5161 acgcattagt caggcgaagc tgcttttgag caccacccgg atgcctatcg ccaccgtcgg
     5221 tcgcaatgtt ggttttgacg atcaactcta tttctcgcga gtatttaaaa aatgcaccgg
     5281 ggccagcccg agtgaattcc gtgccggttg tgaagaaaaa gtgaatgatg tagccgccaa
     5341 gttgtcataa ttggtaacga atcagataat tgacggcttg acggagtagc atagggtttg
     5401 cagaatccct gcttcgtcca tttgacaggc acattatgca agcattgctg gaacacttta
     5461 ttacccaatc caccgtgtat tcattgatgg cggtggtgtt ggtagccttt ctggagtcgc
     5521 tggcgctggt cggtttgatt ctacccggta cggtgctaat ggcgggactg ggggcgctga
     5581 ttggcagtgg cgagttaagt ttctggcacg cctggctggc agggatcgtc ggttgtctgc
     5641 tgggcgactg gatctccttc tggctgggct ggcgttttaa aaagccgttg catcgctggt
     5701 catttctgaa gaagaacaaa gcattactcg ataaaaccga acatgcgctg catcagcaca
     5761 gcatgttcac cattctggtc ggtcgctttg ttggcccgac gcgtccgctg gtgccaatgg
     5821 tggcgggaat gctggatctg ccggtggcaa aattcattac gccgaatatt atcggctgcc
     5881 tgctgtggcc accgttttac ttcctgccag ggattctggc gggtgcggcg atcgatattc
     5941 cagccggaat gcagagcggc gagtttaaat ggttgctgct ggcgacggcg gtatttttgt
     6001 gggttggtgg ctggctgtgc tggcggttat ggcgcagcgg taaagcgact gaccgtttga
     6061 gtcattattt gtcgcgcggt cgtttgttgt ggctggcacc gttaatttct gccgtcggtg
     6121 tagtggcgct ggtggtgtta attcgccacc cgttgatgcc ggtgtatatc gatattttgc
     6181 gtaaagtggt tggggtttag gggatagcct tgtgcgggtt gcctgatgcg acgcttgccg
     6241 cgtcttatca ggcctacaaa acgcactgtc cgtaggtcgg ataaggcgtt cacgccgcat
     6301 ccgacagtgc atactaaccc gtaatcccca atattgccga agcactcgcc ttaccgctca
     6361 acaactcatc ggtcataccc tgccaggcga tgcgcccgtc ggcgaccacc accgagcgcg
     6421 tggcgatccg cgccgcatct tccacactgt gcgacaccat caatagcgtc attttttgct
     6481 gttggcagct cgtgctcacc agcgtcaaca tctcctgacg taacgccgga tcgagcgcag
     6541 agaacggttc atcgagcaat aaaatcggct gttcgcgcac cagacaacgc gctaacgcca
     6601 cgcgctgtcg ctgaccgcca gaaagctcgc ccggtaagcg cgccattaaa ttatcaatcc
     6661 ccatctgacg ggcaatcgcg tgcattttct cctgttgtgc cgcgttcagc ttcaatcctg
     6721 gattaagccc cagcccgatg ttctgcgcga ccgtcaggtg actgaacagg ttgttttcct
     6781 gaaacagcat cgacaccgga cggcgtgacg gcggcgtcgt ggtgtgatca acgccgtcga
     6841 tagtcagcga accgctggct ggcgtcagaa aaccggcgat caaattcagc agggtacttt
     6901 tacccgcgcc gcttggcccg aggatcgcca cctgctcgct gcgttccacc gttaagctaa
     6961 aacgcatcgg caaatggtgg taaagccagg tgatatcagt cagttttaac atttcgcccc
     7021 ggtagttttt caatcacggt aaacagcaga aaacagagta gcagcagaat taacgcggtg
     7081 accgcgccgt cctggctgcg ataggagcca atttgctggt agagataaaa cggcagggtg
     7141 cggaaatcat cgttaccaaa caacgccacc acgccaaaat caccaatcga tagcacacaa
     7201 gcaaaggcca gtgcctgcgc cagtgggcgt ttaagggcgc gcagctccac cacttttaag
     7261 cgtgaccagc cttcaatgcc cagcgactga cacaacatac tgtaacgcgc ggtgatatcg
     7321 cgcatcgggt tttccagcac tttcagcgca taagggatcg ccattaacgc attggtgaaa
     7381 atcacaatgc cgtcagcaga ttgtggcagg ccgatagtgt tgttgagcag taaaaagaag
     7441 ccggtagcca gcacaatccc cggcatcgcg aggatcaaca tgccgctcat ctccagcgcc
     7501 tgacccgcca gcattttctg ccgcgcccgc agttcgcgac tgctccatag cagcatcatg
     7561 gtcagcacta cgcataatac gcctgccgcc agcgcaatac gcaacgaggt ccacagcgcc
     7621 tgccacagca ccggctgcgc cagcacttcc ggcaactggc gatttatccc atcgacgagc
     7681 accgccagta acggtggcag caacagcaac agcgccagca caattaacac cgtgtcgcaa
     7741 atgcggctat gcagacgatc gtccgggtcg cgccagcctt gcagcagcgt agtgccgggc
     7801 gcaatggcct tactcaatcg ctgactcaac agcaccagcc caaggcaaca caccatctgg
     7861 atcagcgcca gcatcgccgc gcgggcagga tcgtagtcgt aactcagcgc ctgataaata
     7921 gccagctcga tagtcgtcgc ttgcggaccg ccgcccagcg acaacacggt ggcgaagctg
     7981 gcgaaacaga gcataaagat aagcgcggca accggcggga tttgtcgccg taaccacggc
     8041 cattcgacga agcggaaaaa atgccagccg cgcatcccaa gctgggcggc aagctgacgt
     8101 tgttccccgg gaatgttttc cagtgcctgg agtaataagc ggctcgccat cggcagatta
     8161 aaaaatacat gcgccagcaa aatgccttgc aggccgtagg gcgaaaacgt ccactccaga
     8221 ccaagcgatt gccagagcga tgccagccag ccctggcgac catagacgct aagaatgccg
     8281 aaaacagcaa ccagcaccgg gaggatcaag gtcatggcac acagacgcaa cagcgccagc
     8341 cgtcctggaa agcgcctgcg gtagagggcg cgggcaagga atatcgcggg cacgacagag
     8401 agcagcgccg agagaaacgc ctgccagaag gagaagcgca ccacatgcca caggtagctg
     8461 tcctgccaga ttgccaccca gtcaccctgc ggcgcgttcc accacagggc gagaaacgca
     8521 gccagtgcaa ccgccaccac cagcgtggcg gcgcttattc ctggaattaa ccagccggga
     8581 attaacggct gacggcgcgt tgccattcgc taatccatgc ctgacgttgt gccgccactt
     8641 cggctggcgt gaactccagc gtggttgccg gtttggtcaa ttgttcaaaa ccggcaggca
     8701 gcgtgacgtt tgccaccgga tacatccagt tgccggttgg gatcgcattc tgaaaagccg
     8761 gagaaaccat aaactggaga aatttttgcg ccagctccgg ctgcttgctg gcagcggtgc
     8821 gggcggcgac ttccacctgc agatagtgac cttcgctgaa gtttgcggcg gcgtagttat
     8881 ctttcttctc ttcgagaatg tgataagccg gagaggtggt gtaactcagt accagatcgc
     8941 tttcaccttt taaaaacagg ccgtaggctt cgctccagcc tttggtgacc gtgaccgttt
     9001 tcttcgccag tttctgccag gcttgtgggg cgtcatcgcc atagactttt tgcatccaca
     9061 acagcaggcc cagccccggc gtactggtgc gcggatcctg ataaatcacc cgccagtttt
     9121 gatcgctctc aacaagctct ttcaggcttt gcggcggatt tttcagtttg ttcttgtcat
     9181 agacgaaggc gaaatagccg taatcaaacg gtacgaaagt gtcatttttc cagccgccgg
     9241 gaacgttaac ggcatccgct gccacgccgc ttttggcaaa tagtccggtt ttactggcgg
     9301 cgtctaacag gttgttatcc agccccagca ccacatcggc tttactgttt ttgccttcca
     9361 tccgcagacg gttgagcagc gaaacgccat cttccagcgc caccagtttc agttcgcagt
     9421 tacagtcggc ttcaaaagct tttttgatct ttggaccagg cccccagtcg gcggcgaagg
     9481 aatcgtaggt ataaacagtc agaacgggtt tagcgaaaac gggcgctgtg cacagcaaca
     9541 gcaaggggag acattttttt aacactttgc acctcaaaaa agagtggcaa aggacttgag
     9601 aaggagcctc aaatcccttc gccggcgtta tccggatcag gttcgacggg tattttctca
     9661 gcgcacgcgt ctgcgtggca ccccgttgag aacggcgtta gtgtagtgat tttgttatca
     9721 accagcaatc atggatccgg cggcgcaaac cacgctgatt taaaatcgaa ccagccgagg
     9781 gtattcatac gcaggccgcg catactgcgt tgcccctgaa tgatcagcca gtggtggata
     9841 agcggcacca ttgctttgct ggcgaccagt tgctggcacc agttcgccag gtttatttcg
     9901 ccattgcgcc agcgggcggc gtcggcttgc cagtcgatcg gaatacagtg ttgtagcagc
     9961 ggcacctcgc acaggtgcgc gaatagcgaa aaatccagcg gcagcgtaaa gttggcgctg
    10021 ttaagccaga tatcgctctc gatctctcct tcatgccact gatcgtagct gatctctttg
    10081 atttccagcg tgacctggtg acttgccaga atctgctgca tgatcccggc aatcacccta
    10141 tgctcactgt gatcctgata aaaggtcagg gtgaggcttt ccagaccagc cggtttttcg
    10201 ctctttatgg tgcgggtatg gtgccaacgg ggaagcagcc cataggccgg aaaccacaac
    10261 tgctggtact gttcctcggc gaaataaacc agattggtcg gcgaaagcac atagcttatc
    10321 cagtctctga cttgctgatt cgccccgcga tgggtacggc tgtcgaacag taaatagtaa
    10381 caaccttctt caaggcggct ttcaatctct ttttcctcgt cttgcggtcc tttcagcatc
    10441 aggcctcccg cggggtcatc agcaatttcc ggcaagaccc agacgttaac ttcgtcgatt
    10501 aatgcccgat aaccaaagaa gtcgtcgaat gcctgaattt tcagttgatt gctgctgttg
    10561 cggatcaccg cataaggacc cgttccgatg ggatggctgg caaagttgct gagggtttcc
    10621 cattcgcgcg gcaggatcat cgccggaact tgccccagca gtaacggtaa ccagcggtcc
    10681 ggttgcgtga gatggatatc cagcgtccag ggcgtcggcg acacaatgtc agcaatatgc
    10741 gaatagagcg gcagcgtatt gattcgtttt aaagaggcga tcacgtcgtc catttccagt
    10801 tcacgaccat gatgaaaatg gactcctgga cgcaaaaaga aacgccagtg aagcggtaaa
    10861 atttgctgcc agtggtgggc gatgtccgct tccagttccc cattttcctc atttatgcgc
    10921 gttagcgaac tgaagatttg ccgggcgata tgggtttcgg aacgacgcaa tgcactgccg
    10981 ggcagcagat tacgcaacgg acgatagtag agaacgcgca ggatgtgccg cccctggcgg
    11041 aagctgcgtc ccagatgaga aaccaacatt tgccgcacag ccgctttgtc gccaaccagt
    11101 tgcaccagtt gatcgatacg atcctgctcc agcaggtcct ctgctcgctg ttgctgaagc
    11161 gccagcccgg tatagaggaa tgtcagacgc gagcgtttgc cgcgcccgac ttccgcttcc
    11221 cacgtcagcc agccgcgatc ctgcatggtg ttgagcaggg tgcgcatatg acgacgcgag
    11281 cagctcaata acgctgccag ttcgttgagc gtcgtgtcct gcgatttacc ctcgcagcat
    11341 tgccacaggc ggatgaactg ttgttgcaga cgagcagatg gcataaaagg ggaactcctg
    11401 cgcaaaagac agcaatttta ttttccctat attaagtcaa taattcctaa cgatgaagca
    11461 aggaggtgcc tcatgcgtca gttttatcag cactatttta ccgcgacagc gaagttgtgc
    11521 tggttgcgtt ggttaagcgt cccacaacga ttaaccatgc ttgaagaact gatgcaatgg
    11581 gaggggagtc attctgacta ctgatttgcg gacatcatgt gtgactgagt attggtgtta
    11641 atcgccacgc cagcagtgat tatctgctgg cttttttctt tttggcgatt gagtgccgga
    11701 cggtgtacaa actaacgcat aaacgccggt tgcttctctt cataagacgc aattgcgtcg
    11761 tcgtgctgca gagtcaggcc gatgctgtcc agaccgttca tcatgcagtg gcggcggaag
    11821 gcatcgatgg taaagcgata agttttctct cccgctttca cctcttgcgc ttccaggtct
    11881 acatcaaaat gaatccccgg attggcctgc accagcgcaa acagttcgtc cacttctgcg
    11941 tcgcttaatt tcaccggcag cagctggttg ttaaagctgt tgccgtagaa gatgtcagca
    12001 aaactcggtg caatcaccac tttaaaaccg taatcggtca atgcccacgg cgcgtgctca
    12061 cgcgaggaac cgcagccgaa gttttctcgt gccagcaaaa tggaagcgcc ctgatactgc
    12121 gggaagttca gcacgaagtc cgggtttggc tgttggcctt tttcatccag aaaacgccag
    12181 tcgttaaaca gatgcgcgcc aaaacccgta cgggtcactt tctgcaaaaa ctgtttcggg
    12241 atgattgcat cggtatcgac attggcggca tccagcggaa ccaccaggcc tgtgtgtttg
    12301 ataaatttct ctgccatggt gtgctcctta tttaatgttg cgaatgtcgg cgaaatgtcc
    12361 ggtcacagca gcagcggcag ccattgccgg gctgaccaga tgggtgcgcc ctccacgccc
    12421 ctggcggcct tcaaagttac ggttgctggt ggaggcacaa cgctcacccg gattcaggcg
    12481 atcgttgttc atcgccagac acattgagca acctggcaag cgccattcaa aaccggcttc
    12541 aataaagatt ttgtccagac cttccgcttc cgcctgggct tttaccgggc cagagccggg
    12601 aaccaccagt gcctgcacgc ctggcgcgac tttccgtcct ttggcgattt cagctgccgc
    12661 gcgtaaatct tcaatacgtg agttagtgca ggaaccgata aacactttgt cgatagccac
    12721 ttctgtcagc ggaatacccg gtttcagccc catataggcc agcgcttttt ctgccgacgc
    12781 gcgttcaacc ggatcggcaa acgaagccgg atcgggaatg ttgtcgttca cggaaatcac
    12841 ctgacctgga ttggtgcccc aggtgacctg cggtgaaatt tcttctgcct gcaaagtcac
    12901 aacggtatcg aaagttgcgc cttcgtcggt ttgcagagtt ttccagtagg caacggcgtc
    12961 gtcgaaatct ttgcctttcg gcgcatgcag acggcctttg acatagttaa aggtagtttc
    13021 gtccggtgca accagaccgg ctttcgcgcc catttcgatt gccatattgc acagggtcat
    13081 acgaccttcc atgcttaaat cacggattgc ttcgccgcaa aactccacca catgcccggt
    13141 gccgcctgcg ctaccagttt taccgataat cgccagcacg atatctttcg ctgtaatgcc
    13201 cggcgcggct ttgccctgga cttcaatttt catggtcttc gcacgaccct gtttcagggt
    13261 ttgcgttgcc agtacgtgtt caacttcgga agtgccgata ccaaaggcta gtgcgccaaa
    13321 cgcgccgtgg gtggcggtat gcgagtcgcc gcagacaatg gtcatccccg gcaatgtgac
    13381 gccctgttcc ggccccatta cgtggacgat cccctgatac gggtgattca ggtcatacag
    13441 ctcgacgcca aattctttgc agtttttgat cagctcctgc atctggatgc gcgccatttc
    13501 accgcaggca ttaatgtctt tggtctgagt agagacgttg tgatccatag tggcgaaggt
    13561 tttgcctggc tgacgtaccg ggcgaccgtg ggcgcgcaga ccatcgaacg cctgcggtga
    13621 ggtcacttca tgcaccaggt ggcggtcgat atataacagc ggggtttcgt tttcggcttc
    13681 gtacactacg tgagcgtcga acaatttttc gtataacgtt ttagccatga ttacacccct
    13741 tctaccacat agcgggcaat gatatcgccc atttcattgg tactaacggc ggcagcgcca
    13801 cgggctaaat ccccggtgcg aatgccttct tctaatgcgc ggttaatggc gcgttcaatg
    13861 gcgcaagccg catcatctgc atccaggctg tagcgcagca gcagtgccag cgacagaatt
    13921 tgcgcaatcg ggttggcgat gtttttgcct gcgatatctg gtgctgagcc gcctgccggt
    13981 tcatacaaac caaaaccttg ctcgttcagg ctggcggaag gcaacatccc catcgagcca
    14041 gtgatcattg cgcactcgtc agacagaatg tcgccaaaca ggttggaaca cagcaggacg
    14101 tcaaactgtg atggatcttt aatcagctgc atggtggcgt tgtcgatgta catatgcgcc
    14161 agttcgacat ccgggtattc cgtggcgatc tcgttgacga tttcacgcca taaaatagag
    14221 gattgcagca cgttggcttt atcgatcgac gtcactttat ggcgacgttt acgagcagat
    14281 tcaaacgcga tgcgggcaat acgctcgatc tcaaaacggt gatacacctc ggtatcaaac
    14341 gctttttcat attgtccgct accttcgcgg ccttttggct gaccgaaata gatgccgccg
    14401 gtcagttcac gcacacacag gatgtcgaag ccgttagcgg caatgtcagc acgcagtgga
    14461 cagaatgctt ccagcccctg atacagtttt gccggacgca ggttgctgaa taatttgaag
    14521 tgcttacgca gaggcagcag cgcgccgcgt tctggttgct ggtctggtgg taaatgttcc
    14581 cacttcggac cgcctaccga gccaaacagc acggcatcgg cttgctcaca accttcaacc
    14641 gtcgcaggcg gcagtggttg cccgtggtta tcaatggctg cgccgcctac atcgtaatgg
    14701 ctggtggtga tacgcatcgc aaatcggttg cgcacggcat ccagcacttt cagcgcctga
    14761 gtcatcactt ccggaccaat accgtccccc ggcaatacgg caatatggta attcttcgac
    14821 atcacacggt ttccttgttg ttttcgttgt gttgagcttt gcgttgcaac tctttttcga
    14881 cttctgcggc acgccagata ttgttcagta cgtgcaccat ggctttggcg gaggactcga
    14941 caatatcggt ggccaggccg acgccgtgga agcggcgacc gttgtagttg gcgacaatat
    15001 ccacctgacc cagcgcatct ttaccgtgac ctttggcggt caggctgtat ttcaccagtt
    15061 cgacgttata gtcagtgatg cggtttatcg cctggtagac ggcatcgacc ggaccgttac
    15121 cgttggcggc ttctgctttg acttcttcgc cgcaggccag tttgacggcg gcagtggcaa
    15181 tatcgttaga acctgactgc acgctgaagt aatccagacg gaaatgctcc ggctcttctt
    15241 gctgcttacc gatgaaggcc agcgcctcca gatcgtaatc aaacacctga ccttttttgt
    15301 ccgccagctt caggaaggca tcgtacaaat tgtctaaatt atattcactt tctttatacc
    15361 ccatctcatc catgcggtgt tttactgccg cacgccccga acgagaggtc agattcagct
    15421 ggatttggtt caaaccaata gattctggtg tcatgatttc gtagttttcg cggtttttca
    15481 gcacgccatc ctggtggata ccggaggagt gtgcgaatgc gccgctgcca acaatggctt
    15541 tgtttgccgg gatcggcata ttacaaatct ggctaactaa ctggctggtg cgccatatct
    15601 cctggtgatt aatagcggtg tggacgttga ggatatcctt acgaactttg atcgccatga
    15661 tgacttcttc cagcgaacag ttaccggcac gctcgccgat cccgttcatt gcaccttcca
    15721 cctggcgtgc accggcatgt accgccgcca gtgagttgcc gaccgccagg cccagatcgt
    15781 cgtgggtatg tacggaaata atggctttgt cgatgttggg tacgcgttca tacagcccgc
    15841 tgatgattcc ggcgaactca aatggcatgg tgtagcccac ggtgtccgga atgttgatgg
    15901 tggtggcacc ggcattaatc gccgcttcga ccactcgcgc cagatcggca attggggtgc
    15961 gtccggcatc ttcgcaagaa aattcaacat catcggtgta attacgggcg cgtttcacca
    16021 tatagatagc gcgttcgatc acttcgtcca gcgtgctgcg cagcttggtg gcgatgtgca
    16081 ttggcgaagt ggcaataaag gtatgaatac ggaaggcttc ggcgactttc agggattcgg
    16141 ctgccacgtc aatatctttt tccacgcagc gagctaacgc gcatacacgg ctatttttaa
    16201 cctggcgagc gatggtctgc actgattcaa aatcacccgg cgaagagacg gggaaaccga
    16261 cttccatcac gtcaacaccc atacgctcaa gggccagcgc aatttgcagt ttttctttca
    16321 cactcaagct tgcctgtaac gcctgttcac cgtcgcgcaa tgtggtatcg aaaataatga
    16381 cttgctggct catggtttgg gtccttgtct ctttttgagc gcctcgcttc gggcataaaa
    16441 aaacccgcgc aatggcgcgg gttttttgtt tgactgagtg ctgacttaat tctgaatgcc
    16501 gctcaccagt ctaccgcgca aagaagatgc gtttagtagt agtagaccga taaagcgaac
    16561 gatgtgagtc attaaatcag ctccagatga atgcgatatg cttttagagt tactggatac
    16621 caaaacggat gtcaaccctg atgcaacaaa aacgtccagc cagcgtgagt tctgcatccg
    16681 tcaaattagc taattgtgct gcggtggtta aaacaagcga tattaatttc tgcttaacca
    16741 ccgccgcttt tcatcggttg acatatttca gaataaattt ttgcatctaa tcgacgaggg
    16801 aaagggggat aaaatgcacg cgttacaaaa ccta
//
LOCUS       DAZUCC010000107        16606 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZUCC010000107 DAZUCC010000000
VERSION     DAZUCC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.107"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            116..634
                     /gene="yfiR"
                     /locus_tag="ACHY09_002916"
     CDS             116..634
                     /gene="yfiR"
                     /locus_tag="ACHY09_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFS3885868.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            624..1850
                     /gene="dgcN"
                     /locus_tag="ACHY09_002917"
     CDS             624..1850
                     /gene="dgcN"
                     /locus_tag="ACHY09_002917"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFS3885869.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNRPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1866..2348
                     /gene="yfiB"
                     /locus_tag="ACHY09_002918"
     CDS             1866..2348
                     /gene="yfiB"
                     /locus_tag="ACHY09_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3885870.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     ALSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2425..2772)
                     /gene="rplS"
                     /locus_tag="ACHY09_002919"
     CDS             complement(2425..2772)
                     /gene="rplS"
                     /locus_tag="ACHY09_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS3885871.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2814..3581)
                     /gene="trmD"
                     /locus_tag="ACHY09_002920"
     CDS             complement(2814..3581)
                     /gene="trmD"
                     /locus_tag="ACHY09_002920"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS3885872.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3612..4160)
                     /gene="rimM"
                     /locus_tag="ACHY09_002921"
     CDS             complement(3612..4160)
                     /gene="rimM"
                     /locus_tag="ACHY09_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS3885873.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4179..4427)
                     /gene="rpsP"
                     /locus_tag="ACHY09_002922"
     CDS             complement(4179..4427)
                     /gene="rpsP"
                     /locus_tag="ACHY09_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS3885874.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4564..5925)
                     /gene="ffh"
                     /locus_tag="ACHY09_002923"
     CDS             complement(4564..5925)
                     /gene="ffh"
                     /locus_tag="ACHY09_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS3885875.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6092..6883
                     /gene="ypjD"
                     /locus_tag="ACHY09_002924"
     CDS             6092..6883
                     /gene="ypjD"
                     /locus_tag="ACHY09_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFS3885876.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6904..8190
                     /gene="yfjD"
                     /locus_tag="ACHY09_002925"
     CDS             6904..8190
                     /gene="yfjD"
                     /locus_tag="ACHY09_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFS3885877.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8244..8837)
                     /gene="grpE"
                     /locus_tag="ACHY09_002926"
     CDS             complement(8244..8837)
                     /gene="grpE"
                     /locus_tag="ACHY09_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS3885878.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8960..9838
                     /gene="nadK"
                     /locus_tag="ACHY09_002927"
     CDS             8960..9838
                     /gene="nadK"
                     /locus_tag="ACHY09_002927"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFS3885879.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9924..11585
                     /gene="recN"
                     /locus_tag="ACHY09_002928"
     CDS             9924..11585
                     /gene="recN"
                     /locus_tag="ACHY09_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS3885880.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKHLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELALLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11734..12075
                     /gene="bamE"
                     /locus_tag="ACHY09_002929"
     CDS             11734..12075
                     /gene="bamE"
                     /locus_tag="ACHY09_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFS3885881.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12137..12427)
                     /gene="yfjF"
                     /locus_tag="ACHY09_002930"
     CDS             complement(12137..12427)
                     /gene="yfjF"
                     /locus_tag="ACHY09_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFS3885882.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12417..12854)
                     /gene="ratA"
                     /locus_tag="ACHY09_002931"
     CDS             complement(12417..12854)
                     /gene="ratA"
                     /locus_tag="ACHY09_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFS3885883.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            13025..13507
                     /gene="smpB"
                     /locus_tag="ACHY09_002932"
     CDS             13025..13507
                     /gene="smpB"
                     /locus_tag="ACHY09_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS3885884.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13722..14084
                     /gene="ssrA"
                     /locus_tag="ACHY09_002933"
     tmRNA           13722..14084
                     /gene="ssrA"
                     /locus_tag="ACHY09_002933"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14347..14595
                     /locus_tag="ACHY09_002934"
     CDS             14347..14595
                     /locus_tag="ACHY09_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS3885885.1"
                     /translation="MRIEICIAKEKFSKMPKGSVPALQQEMLRRIRKRYDDAEVIVKS
                     ASNDGLTILRAVDKESAHEFVQETLQEAWETADDWFIR"
     gene            14604..14702
                     /locus_tag="ACHY09_002935"
                     /pseudo
     CDS             14604..14702
                     /locus_tag="ACHY09_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(15008..15679)
                     /locus_tag="ACHY09_002936"
     CDS             complement(15008..15679)
                     /locus_tag="ACHY09_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFS3885886.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYESQSLFKGDTLKV
                     VYNSAGVITGISRVASVLWPVGQSVAEVEDTEENRKADTSGRWGFDGEKITDLLTVEK
                     ARVMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEGFFW
                     TDAENNDVPVTAEALIALSEAAEQAMFSKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWGQE"
     gene            complement(15722..>16606)
                     /locus_tag="ACHY09_002937"
     CDS             complement(15722..>16606)
                     /locus_tag="ACHY09_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFS3885887.1"
                     /translation="AWLKEKGAFDQPYWMMKASLLATMNKVITDVGPGKLNLGGCAIE
                     VMGTYNAAIVRVTIGEYGATGFINGTVCTCTVYGDTQRFHWRVDYSTKNKPDAVSQQD
                     ASTTQKGVVQLSSDTASNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGE
                     KGETGPAGPRGEQGPKGEKGDPGPKGERGATGPAGPRGETGPMGPPGASDGKSRVVGI
                     RLGNEQTYRADESTGFLSISLRTGSVLTGLESRGADGVMSVSYRPLQVTTDGRQWRTV
                     SVGEYVSSGAGGLEYFSP"
BASE COUNT         4096 a         4479 c         3871 g         4160 t
ORIGIN      
        1 tactcattgt gttttatttc tcacattgat gacgaccgca tgaaagaatg atattttaat
       61 gagcattctg aaaaaatatc gatgcatttc gagcgaagat ggtgaggatc cctgaatgcg
      121 tttttctcac cgactgttct tactgctgat tctcctgctg acgggagcac caatactcgc
      181 tcaggaacct tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta
      241 cacccgctgg cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacattt
      301 tagtaacgcc ctacaagaga atggcacgac atctttgcct tatctacctg tcatcattca
      361 cactcaacaa gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc
      421 gacattccag atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga
      481 acaaaatacc gagtgcatta ttggtagcgc cttttgtcta atcatccaca ataatgacgt
      541 cagatttgcc gtaaacctgg atgccttatc gcgtagcggt gtaaaggtca acccggatgt
      601 cttaatgctc gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca
      661 cgtttaaaag agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga
      721 tctggttgct gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc
      781 tggcactgac agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg
      841 gccctgcagc aactgaaaca ctggcagcgc tgggccagca aggccaattt tcaactgcag
      901 aagtgcgtga taagcagcaa aatattctgg catcctggca ttacacccat aaggaaccag
      961 gcgatacttt cagcaatttc ataagccact ggctcttccc tgcccccatc attcagccga
     1021 ttcgtcacaa tggtgaaacc attggcgaag tacgcttaac tgctcgcgac agttcaatca
     1081 gccattttat ctggttttcg ctcgctgtcc tgaccggttg tattctgctg gcatcaggca
     1141 tcgcaattac cctcacccgc catttgcaca atggcctggt ggaagcgctg aaaaatatca
     1201 ccgatgtcgt acatgatgtg cgttctaacc gcaatttttc ccgacgagtt tcggaagaac
     1261 gtatcgctga gtttcaccgc ttcgctctcg acttcaacag tctgctggat gaaatggaag
     1321 agtggcagct tcgtttacag gccaaaaatg cgcagctttt acgtaccgcg ctacatgacc
     1381 cattaaccgg gctggctaac cgcgcagcgt ttcgtagcgg catcaacacg ttgatgaaca
     1441 attccgatgc ccgaaaaacg tcggcgttac tatttcttga tggcgataat ttcaaatata
     1501 tcaatgatac ctggggtcat gcgacgggcg atagagtctt gattgaaatc gcaaaacggt
     1561 tagctgaatt tggcggactg cgacataaag cataccgcct gggcggcgat gaattcgcga
     1621 tggtgctcta tgatgtacag tcggaatctg aagtgcagca gatatgctca gcactgacac
     1681 aaatctttaa tcgcccgttt gatcttcata atggtcatca gaccaccatg acattaagca
     1741 ttggttacgc gatgaccatt gagcatgcct ctgcggaaaa attacaagag ctggccgatc
     1801 acaatatgta tcaggccaaa caccagcgtg ccgaaaagct ggtgagataa caaggatata
     1861 tcgatatgat aaagcacctg gtagcacccc tgattttcac ctcactaata ctgactggct
     1921 gccagtcccc tcagggaaag tttactcctg agcaagtcgc cgctatgcaa tcttatggat
     1981 ttactgaatc cgccggcgac tggtcgctgg gcttatcaga tgccattctg ttcgcaaaaa
     2041 atgactacaa attgctcccg gaaagccagc aacagatcca aaccatggca gccaaattgg
     2101 cctcgacagg gctaacgcat gcccgtatgg atggacacac cgacaactat ggtgaagaca
     2161 gttacaacga agccttatca ttgaaacggg cgaatgtcgt ggccgatgca tgggctatgg
     2221 gtggacaaat tccgcgcagc aatctcacca cacagggttt aggaaaaaaa tatcccatag
     2281 ccagtaacaa gaccgcccag ggccgcgccg agaaccgccg cgtcgcagtg gtgattacta
     2341 ccccttaaaa cacgagcaaa cagataaaaa aagggccagc caattggcca gcccttctta
     2401 acaggatgtc gcttaagcga aatcttagtt aagacgctct ttgatacgag cagccttacc
     2461 agtacgctca cgcaggtagt acagtttagc tttacgaaca gcaccacgac gtttgacaga
     2521 aatgctgtca actaccggag agtgagtctg gaagacacgc tcaacgcctt cgccgttgga
     2581 aattttacga acagtgaatg cagagtgcag accgcggtta cgaatagcga taaccacgcc
     2641 ctcgaatgcc tgcagacgtt ttttggaacc ttcaacaacc catactttca cttccacggt
     2701 atcacccgga cggaaggaag gtacgtcctg cttcatctgc tcttgttcaa gttgcttaat
     2761 aatgttgctc ataatttaat ctcttatcct gggtaaactg atatctcggg ggcttacgcc
     2821 atcccatcat gtttatgttg ctgttgtgcg tgttccgttt tgaactccgc cagcaacctt
     2881 gcttgctctt cagtcagagc caggttttcc agaagttcag gtcttctaag ccaggtacgg
     2941 cccagcgact gtttcaaacg ccagcgacgt atctcggcat ggttgcccga cagtaacact
     3001 ggcggaactt ccatcccttc taacacctca ggccgcgtat agtgcgggca atccagcaat
     3061 ccttcagcaa aggaatcttc cgttgccgag gcttcatgtc ccagtactcc cggaataaac
     3121 cgggaaacgg agtcaatcag cgtcattgct ggtaactcac caccactgag aacgtaatcg
     3181 ccgattgacc attcttcgtc aatttcggtt tggatcacgc gctcatctat accttcgtag
     3241 cgaccgcaca ccagaatcaa tttttgattc gttgccagtt cgctgacgcc cgcttgatca
     3301 agcttgcgtc cctgtggtga cagataaatc acctttgcgc cttcacccgc cgcggctttt
     3361 gctgcatgaa tggcgtcccg caagggttgc accatcatta acatccccgg tccgccgccg
     3421 taaggacgat cgtccacggt acggtgccgg tcatgcgtga agtcgcgagg actccagctc
     3481 tggatgctca gcaggccatt tttaactgcc cggccagtta ccccgtaatc ggtaattgcg
     3541 cggaacattt caggaaacag gctaattatg ccaatccaca tagcgccgtc ttttaccgtt
     3601 tatccggtgg tttaaaaacc aggatcccaa tctacttcga ttgaacgagt agtgagatcg
     3661 actttcttga taacctgccc atcgaggaac ggtacgagac gttccttgat accaaacgca
     3721 tctttcaggt ttgccttaat gacgagaacg tcattagatc cggtttccat catatcgacg
     3781 actttaccga gatcgtagcc ttcagtggtt actacctggc agcccatcaa gtctttccag
     3841 tagtagtcac cctcttcaag ctgaggcaac tgcgatgaat ccacgacaat ttcacaattc
     3901 gtcagcaggt tcgccgcatc acgatcgtca acgcctttca gcttgatgat catgtcctga
     3961 ttgtggtgct tccagctttc cagctggact tgctgccact gacccgcctt ctggataaac
     4021 cagggctgat agtcaaaaat gctttcggcg tcttcggtgg aagaaaacac tctgagccac
     4081 ccacgaatac cgtaagacga acccattttt cccaaaacga tgggatcaac aggtgcttgc
     4141 gcggtgagtt gtttgctcat catgaccacc gtgacagatt aagctgcttt gtttacttct
     4201 ttgatcagcg cagcaacgcg atcagaaata gttgcgccct ggccaaccca gtgagcgatg
     4261 cgatccagat ccaggcgagt gccttcttct ttttcgctag cgattgggtt gaagaaacca
     4321 acgcgctcga tgaagcgacc gttgcgtgca ttacggctgt cagcgacaac aacctggtag
     4381 aacggacgct ttttagcgcc gtgacgtgct aaacgaatag ttaccataac atcctcttgt
     4441 gtgaataaaa caaccggacc ccatcgagga acggagtccg gtgtcatatt aaaagcccga
     4501 aaattttact gatttctgga gaaaatgcaa tcagcagtta ataactctgt cgcataaggt
     4561 catttagcga ccaggaaatc ccggcggcat catacccttc atgcttctca tcatcttcgc
     4621 cattccgccc ttcttcattt tcttcatcat gcgctgcatg tcgtcgaact gtttcagaag
     4681 acggttaacg tcctgcacct gcataccgca accggcagcg atacggcgtt tacgcgaacc
     4741 tttgatgatt tctggcttag cgcgctcttt catcgtcatc gagttgatga tggcttccat
     4801 acgcaccagc actttatcgt ccatctgcga cttgacgtta tccgggatct gccccatgcc
     4861 cggcagtttg cccatcaggc tggccatgcc gcccatattt ttcatctggc gcagttgctc
     4921 aaggaagtca ttgagatcga agccgtcgcc ttttttcagc ttgctggcta atttctcagc
     4981 ttgcgcgcgg tcaactttgc tttcgatatc ttcgatcagc gacagtacgt cgcccatgcc
     5041 gagaatacgg gaagcgatgc ggtccggatg gaacggctcc agcgcctcgg ttttctcgcc
     5101 aacaccgagg aacttgatcg gtttgcccgt aatgtgacga atagagagcg ccgcaccgcc
     5161 acgagcatcg ccgtcgactt tagtcagtac cacaccggtc agcggtaacg cttcattgaa
     5221 tgcttttgcc gtattggccg catcctgacc agtcatggca tcaaccacaa acagggtttc
     5281 aaccggatta atcgacgcat ggacttgttt gatctcgtcc atcatcgctt cgtcaacgtg
     5341 cagacgaccg gctgtatcca ccagcagcac gtcgtagaat ttcagtttgg cttctttcag
     5401 cgccgcgtta acgatatcta ccggcttctg accaacatca gaagggaaga aatcaacgcc
     5461 cacctgctct gccagcgtct caagctgttt gattgccgcc ggacgataaa cgtcggcaga
     5521 aaccaccagc actttcttct tgtgcttctc gcgcaggaac ttaccgagtt taccaacgct
     5581 ggttgtttta ccggcacctt gcaggcccgc catcagcacg accgcaggcg gttgcgcagc
     5641 caggttcagg gtctggttct cttcgcccat cgccgcaacc agttcgttac ggacaatttt
     5701 gacgaactcc tgccccggcg tcaggctctt attaacttca tgaccaaccg ctttctcttt
     5761 tacgcgattg ataaactcac gcactaccgg cagagcgacg tccgcctcca gcagcgccat
     5821 gcgcacttcg cgcagcgtat cttttacgtt gtcttcagtg aggcgtccac ggccactgat
     5881 attgcgcagc gtgcgcgaca aacgatcggt taaattatca aacattgtct ctcgcctggg
     5941 gtggaaacgg ttggccgcaa tcgcgacaca tcatcagtat tttgccgcag tataacatga
     6001 aggcgtcttt gttgttatgc aacggttgga gcagcgttca cctgacgcta tactgcttct
     6061 ctttcttatt gctcaaactg tcgacattac tatgcccgtt tttgctctgc tcgcgcttgt
     6121 cgcctactcc gtcagtcttg cgctgattgt tcccggtctg ctgcaaaaaa acggcggctg
     6181 gcggcgcatg gctattattt ctgcggtcat tgcgctggtc tgccacgcaa tcgctctgga
     6241 agcccgcatc ctgcccgacg gtgatagcgg acaaaacctc agcctgctga acgttggttc
     6301 attggtcagt ttgatgatct gtacggtaat gaccattgtg gcttctcgca atcgtggctg
     6361 gttgctgcta cccattgtct atgcctttgc gcttatcaac ctggcgctgg cgaccttcat
     6421 gcccaatgaa tacatcaccc atctggaagc aacgcctggg atgctggtgc acattggctt
     6481 atcgctcttt tcctatgcca cgctgattat cgccgccctg tacgcgctgc aactggcgtg
     6541 gattgattac caactgaaga acaagaagct ggcgtttaac caggaaatgc cgccattgat
     6601 gagtatcgag cgtaaaatgt tccacatcac gcagattggc gtggtgctgc taacgctcac
     6661 gctttgcact ggcctgttct acatgcacaa cttatttagc atggaaaata tcgacaaggc
     6721 cgtgctctct atcgtggcgt ggtttgtcta tattgtgctg ctgtggggac attatcatga
     6781 aggatggcgt ggacgccgcg tcgtctggtt taacgttgcg ggtgcagtca ttctgacact
     6841 ggcctacttc ggcagccgaa ttgtccagca gttaatcagc taaacccaga aaaggagttt
     6901 cccctggaac acatttctac tactacgttg atcattattc tgatcatcat ggtggtcatt
     6961 tcagcctatt tttccgggtc cgaaaccgga atgatgaccc tcaaccgcta tcgtctgcga
     7021 catatggcga aacagggtaa tcgctcggcc aaacgcgtcg aaaaattgct gcgtaagcca
     7081 gaccgcctga ttagcctggt gttaatcggc aataacctgg tcaatattct tgcctccgcg
     7141 ctaggcacta ttgttgggat gcgtttgtac ggcgatgcgg gcgtggcaat tgcgactggt
     7201 gtgctgactt ttgtcgtgct ggtttttgct gaggtattgc cgaaaaccat tgccgcgctg
     7261 tacccggaaa aagtcgctta tccgagtagt tttctgctgg ctccgctgca aattttgatg
     7321 atgccgctgg tctggttgct gaatgctatc acccgtatgc tgatgcgcat gatgggtatc
     7381 aaaaccgata tcgtggttag cggctctttg agcaaagaag agttgcgcac tatcgtgcac
     7441 gaatcgcgct cacaaatttc ccgtcgcaat caggatatgc tgctgtcggt gctcgatctg
     7501 gaaaaaatga ccgttgatga catcatggtg ccgcgcagtg aaattatcgg tattgatatc
     7561 aacgatgact ggaaatcgat tctgcgccaa ctctcccact cacctcacgg gcgcatcgtg
     7621 ctctaccgtg attcgctgga cgacgccatc agtatgctgc gtgtgcgtga agcctggcga
     7681 ctcatgtcgg agaaaaaaga gttcaccaaa gaaaccatgc tacgcgccgc ggacgagatc
     7741 tattttgtcc cggaaggtac gccgctcagc acgcagttgg tgaagtttca gcgcaacaaa
     7801 aagaaagtcg gcctggtcgt caacgagtat ggagacattc aggggctggt gacggttgaa
     7861 gatattctgg aagagattgt cggcgatttc actacctcga tgtcgccaac acttgccgaa
     7921 gaggtcacgc cgcaaaacga cggttcggtg attatcgatg gcaccgccaa cgtgcgagaa
     7981 atcaacaaag cctttaactg gcatctacct gaagacgatg cccgcacggt taatggcgtc
     8041 attctggaag cgctggaaga gatccctgtc gctggcaccc gcgtgcgtat tggcgagtac
     8101 gatatcgata ttctcgacgt acaggataat atgattaagc aggtaaaagt ttttcctgtg
     8161 aaaccgctgc gcgagagtgt ggcggagtaa aaaaacggcc cggcattcgc atgctgggcc
     8221 gtgatttatt acgaaagcag aaattaagct tttgccttcg ctacagtcac catcgccgca
     8281 cgaatcgtac gaccgttcag cgtataacct ttctgcataa tgcccagtac gttacctggc
     8341 gcaacgtcat cagattccac cattgcgatg gcctgatgca catttgggtc cagtgggacg
     8401 ttggtttcgg cgatcacttc aacgccaaac ttacgcacaa catccagcat cgacttcagc
     8461 gtcagctcaa taccttcaac catcgcggac atatccgggt tagctttatc agccacttcc
     8521 agcgcacgat ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg
     8581 aatttgtgag ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct
     8641 tttacgcgca aaatgccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga
     8701 ttcgcaattt tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc
     8761 tcaatctctt cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc
     8821 tgttctttac tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc
     8881 ttattatggg gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg
     8941 caacaaggac ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc
     9001 acggcacccc actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg
     9061 ttacgaggtc atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac
     9121 tggcacgctc gcggagattg ggcaactggc agatctcgcg gtagtcgttg gtggcgacgg
     9181 taatatgctg ggcgcggcgc gcacactcgc ccgttacgat attaaagtta ttggaatcaa
     9241 ccgtggcaac ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc
     9301 cgatgtgctg gaaggccact acatcagcga gaaacgtttt ttgctggaag cgcaagtttg
     9361 tcagcaagat tgccagaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg
     9421 caaagtggcg catatgattg agttcgaagt gtatatcgac gagatctttg cgttttcgca
     9481 gcgttccgat ggactgatta tttcgacgcc aacaggctcc accgcatatt ccctctctgc
     9541 gggcggtcct attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca
     9601 tacgttgtca gcacgaccac tggtgataaa cagcagcagc acgatccgtc tgcgtttttc
     9661 gcatcgccgt aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga
     9721 aggtgaagat gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga
     9781 ttacagttat ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt
     9841 ttacgccagc ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg
     9901 gtttttcata caggaaaaca actatgttgg cacaactgac catcagcaac tttgctatcg
     9961 ttcgtgagct tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggcg
    10021 cgggtaaatc tattgcaata gatgccctcg gcctttgtct cggtggtcgc gctgaagccg
    10081 acatggtgcg taccggcgct gctcgtgccg acctgtgcgc ccgtttttct ctgaaagata
    10141 cgccagcggc cctgcgctgg ctggaagaaa accagcttga agacggtcat gaatgtttgc
    10201 ttcgtcgcgt aatcagtagc gatggtcgct cacgaggttt tatcaacggt acagctgttc
    10261 ctctgtcaca acttcgcgaa ctgggccagt tgctgattca gatccatggt cagcacgccc
    10321 atcagttact caccaaaccc gagcaccaaa aattcctgct tgatggctat gccaatgaaa
    10381 cctctctgct ccaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc
    10441 tcgcgcatca tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc
    10501 aattaaaaga acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag
    10561 agtacaaaca tctggcgaac agcggtcaat tgctgactac cagccagaat gcattggcag
    10621 taatggccga cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg
    10681 tgagcgaatt gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag
    10741 ctaccatcca gattgctgaa gccagcgatg aactgcgcca ctactgcgat cgtctggatc
    10801 tcgatcccaa ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac
    10861 gtaaacatca cgtcagccct gaggcattgc cacagtatta ccagtcgcta ctggaagaac
    10921 agcagcaact ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac
    10981 atcatcagca ggcactggaa accgcgcgcg cattacacca gcaacgccag cagtatgcag
    11041 aagaacttgc actgctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt
    11101 ttacgatcga tgttaaattt gacgagcatc acctgggcgc tgacggtgcc gatcgtattg
    11161 agtttcgggt aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg
    11221 gtggtgaatt gtcacgcatc gcattggcaa ttcaggtcat cacggcgcgt aaaatggaaa
    11281 ccccggcact gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg
    11341 tcggcaaact gctgcgtcaa cttggcgaat caactcaggt gatgtgtgtt acccacctgc
    11401 cacaagtcgc gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga
    11461 tgacagaaac acacatgcaa tccctggata aaaaagcgcg gttacaagag ctggcgcgcc
    11521 tgcttggggg cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcgg
    11581 cgtaaacttt tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa
    11641 gcaaaaggtt ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg
    11701 tactgctcgg gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca
    11761 gcagcagtac tattgatgtt gaccgcaggc tgttccactc tggagcgagt ggtttaccgt
    11821 cctgacatca accaggggaa ttatctgacc gctaacgacg tatccaaaat acgcgttggc
    11881 atgacgcaac aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt
    11941 acgaatacct ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa
    12001 acgctgacgc tgacctttaa tagtagcggg gtgttgacca atattgataa taaacctgcg
    12061 ctgagtggta actaatcata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc
    12121 accttttttc tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgcagctctt
    12181 tcggatcggc aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc
    12241 tcagttttgc cggacggcta taaatgccga ctttgttttt agttaaatcg atatcggtac
    12301 gcaattccag caagccactg gcgcgaatag cttcttcaac cgtcgcaccc tcctgcagcg
    12361 tcacacgctg caggtactgc ttctcaggta gcgcataagc cacctcaacg gcaattttac
    12421 ctggcactgt aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagt
    12481 tctttaaaca cgcggccaaa ggcgagttca atcaacttat tggtaaactc aaaatcgaga
    12541 tgaaactcga tacggcacgc ctcctgactc agcggcgtaa acttccatcc accgatcaat
    12601 ttcttgaacg gcccatccac cagattcata agaatacttt ggttactggt caactggttg
    12661 cgggtagtaa acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc
    12721 ccaggagtgg actccagaat ccgacttccg gtacaacccg gcaaaaactg aggataagac
    12781 tgaacgtcat tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg
    12841 ctaatctgag gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct
    12901 gctgtaaaaa aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc
    12961 ttgtcccccg caggattgat atggggtgtt ttcgatttca gattaccgat gattcacgac
    13021 gcttatgacg aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg
    13081 cgcccggcac gaatacttta tcgaagaaga gttcgaggcg ggacttgccc tgcaaggctg
    13141 ggaggttaaa tccctgcgtg caggaaaagc caatatcagc gacagctacg tccttctgcg
    13201 tgacggagag gcatttctgt ttggcgctaa catcacgcca atggctgtgg cctccacgca
    13261 tgtggtgtgc gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc
    13321 attgtacggt cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa
    13381 aaatgcctgg tgcaaagtga aaatcggcgt cgccaaaggt aagaaacagc atgataaacg
    13441 ttcagatatc aaagaacgcg aatggcaggt ggataaagcg cgtatcatga aaaacgccca
    13501 ccgttaaacc tgcactgcaa ttattgatcc gctcctcacc gcgcctccct ctccggcggc
    13561 gcgaatgcac atcttattgg ctatcacatc cgacccaaat gttgccatcc cattgcttaa
    13621 tcgaataaaa atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg
    13681 tcatggcact catgaatctg gtatacttac cgttacacat tggggctgat tctggattcg
    13741 acgggatttg cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca
    13801 aaaaatagtc gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc
    13861 tctctcccta gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc
    13921 gcgtggaagc cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg
    13981 cgtgtccgtc cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga
    14041 cgtaggaatt tcggacgcgg gttcaactcc cgccagctcc accaaatata acaggaccac
    14101 aactagactg taacagtaaa aacaataact tacggacatt gaatagactg taggtagacc
    14161 tgaaaaagac aaaaatatac acgcaatgtg cacgtgcatt ttaaaaaacc ccagatttct
    14221 ccgtctgggg tttttctatt tgtaaaaaag tgaaacaaat tacattgcaa gaatgacgcc
    14281 cttgacactg ttcatccata cagcaaaaat aattcctgta cataaccaca gtgacaggag
    14341 ggtgttatgc gtattgagat ctgtatcgct aaagaaaaat tcagcaaaat gccaaaaggc
    14401 tctgttccag cccttcaaca ggaaatgctt cgccgcatca ggaagcgata cgacgatgct
    14461 gaagtaatcg tcaaatctgc cagtaatgat ggactcacca ttttaagagc tgtagataaa
    14521 gagtccgcac acgaatttgt acaggaaaca cttcaggaag cctgggaaac agctgacgac
    14581 tggttcattc gctgactaaa agttcgtcca catgcaggga attcgcaatg tccggtgata
    14641 atggcgagat caatgaggca tgcaaccgcg cccgggcaaa atacggactc ccggaaatct
    14701 gaaaaacgcg gcaccagaaa cgcgatcggc atcactgcca tgcctgatta tcatgtggca
    14761 atctgttttt gcatggtcat atgaagtttt atcattgtgt tactcactat actctttgga
    14821 ttggggctgg ctgacagccc cttttttagt atccccgtaa aatctgaaaa ttcctgtctc
    14881 aatgaagcga tttgatccgc atcagtgttt ccacaaacat acccgtgcaa atatctgttc
    14941 gtgtaggaag tattttcaca atattgtttg gagcggggag caggctgatg ggctcccctt
    15001 tttttcacta ctcctgtccc cagccaaccc tgtatgccag tatctcatcc gcactggtca
    15061 gcttttccag ctctttcttc atggtgcgct gccgtacgtg gatttccata cctttgctga
    15121 acatcgcctg ctctgccgct tcactcagcg caataagtgc ttctgctgtc accggtacat
    15181 cgttgttttc cgcatccgtc cagaaaaatc cctccggcag tttccccgcc ttcgctgccg
    15241 ccaccgacgg ctcaagacgc gtctgcgttg actttccgta gtcccacttc cggccattgt
    15301 gctcaaacgt gtagttcgcc gcttccatcg catttcgcca ggcgttaatt tcatctccct
    15361 tcatcacgcg cgctttctcc acggtcagca ggtccgtaat tttctccccg tcaaaacccc
    15421 agcgccccga ggtgtcagcc ttccggtttt cttccgtatc ctccacctca gccacactct
    15481 ggcccacagg ccacagaact gacgccaccc tgctgatgcc ggtaatgacc ccggcactgt
    15541 tataaaccac cttcagggtg tcccctttaa acaacgactg actctcatac cagtcctgac
    15601 catcctctga tttcaggtac attgcgcccg gtacatccgg attttccggc gtgtattgtc
    15661 tgaaattctt tatgtgcatc tgtaagtcct catggttaca tatctgatat ctgatgcgct
    15721 tttatggtga aaaatactcc agaccgccag cacctgatga tacatactca ccaacgctga
    15781 ctgttcgcca ctgacgacca tcagttgtca cctgcagggg tctgtaagaa accgacatca
    15841 caccatctgc tccacgactt tccagaccag taagcacact gcctgttctc aggcttattg
    15901 acagaaaacc tgtgctctca tccgctctgt aagtctgttc attacccagc ctgataccca
    15961 caacccgact cttcccgtct gatgcccccg gaggccccat cggcccggtt tcaccacggg
    16021 gaccggcagg tccggttgcg ccacgctcgc cttttggtcc ggggtcgcct ttctctcctt
    16081 tcggtccctg ctcaccgcgc ggacctgccg gacctgtctc ccctttctct cctttcggac
    16141 cgggaacacc acctcctgca gcagcctctt ctgcctttgt tttcgcttca tttgccacat
    16201 ccattgccgc cttcacggcc ttcggcgtgg ctgcctttgt ttcgtcatta ctggccgtat
    16261 cactgcttaa ctgcaccacg cctttctgcg tcgtgctggc atcctgctgg ctgaccgcat
    16321 ccggtttgtt ttttgtactg taatccaccc gccagtggaa acgctgtgta tccccgtaaa
    16381 ccgtacaggt acagaccgtg ccattaataa accccgttgc accgtattcc cctatggtga
    16441 cccggactat ggctgcgtta tacgtcccca tcacctcaat ggcgcagcct cccagattga
    16501 gttttcccgg tccgacatcc gtgatgacct tattcatcgt cgccagcagg gatgccttca
    16561 tcatccagta aggctggtca aaagcccctt tttctttcag ccaggc
//
LOCUS       DAZUCC010000108        16555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZUCC010000108 DAZUCC010000000
VERSION     DAZUCC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.108"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(251..739)
                     /locus_tag="ACHY09_002938"
     CDS             complement(251..739)
                     /locus_tag="ACHY09_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3885888.1"
                     /translation="MAKKTAPLVPGTNQLLAEFGERLKLARLRRKLTAKRVAERAGMS
                     PMTLRSLESGGTGVTIGAYLSVMQILGLEKDLNKLAAEDEQGRQLQDSRLINKTRVSR
                     PAAISHTNRHTGNVIKNDAFDKQFSTSQEKSIDSEVKTEVVTSASLAALLKPIKKPRK
                     GS"
     gene            complement(1047..1856)
                     /locus_tag="ACHY09_002939"
     CDS             complement(1047..1856)
                     /locus_tag="ACHY09_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS3885889.1"
                     /translation="MTNIFTLMNPISSATETLLSQINRICTAQGIPFFIAGATAREIL
                     LHHIHSRSIGRRTRDIDIAVFVDSWPRFDALKNAFLAEGAEVVQGNAHRMIVKGIELD
                     IIPFGGVAEGNQIAWPPEREIIMAVDGFAEVFSHTVVVRLDNSENIPFCSLPGLALLK
                     LFAWRDRGHGSTKDATDLFKIICEYGVIENERIYESPVEGERLNWDPIRMGAFLLGSD
                     IATISEHSSLQDLLHLDKERLIDVIVRQHDVGDAGIEQIINDFWDGMASDG"
     gene            complement(1810..2808)
                     /locus_tag="ACHY09_002940"
     CDS             complement(1810..2808)
                     /locus_tag="ACHY09_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HFS3885890.1"
                     /translation="MKEEQLLSDAIENLPKGFHLQYEHSGRHGKHDAWGKLTGPDGES
                     VIFALEVKFIHRKESLMAVREQMVRFPADFPVLLVCNRLTSALAEYCADSQINFIDTA
                     GNARIQVPGLYVRIEGRYEKKPVSASSRFAEGVMKLLFVLLSSPETLNETYRSLAGKA
                     GISLGMVSKAFDYLEAQRYFRKSKKGRRLINEGELEALWLKDYATALRPKLDSLSLSA
                     PVSWDELTLASGEYWSGEIAAAKLSGGYLIPENGVIFTSHPLLQRRKELGLKPVPGGK
                     LQLIAGFWGNFVLNHKAEAMLCVAELLASGDDRNWETARIINDKYLHLNESDLFSY"
     gene            complement(3436..4683)
                     /locus_tag="ACHY09_002941"
                     /pseudo
     CDS             complement(3436..4683)
                     /locus_tag="ACHY09_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4906..4990)
                     /locus_tag="ACHY09_002942"
     tRNA            complement(4906..4990)
                     /locus_tag="ACHY09_002942"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4954..4956),aa:Leu,seq:caa)
     gene            5186..6205
                     /gene="ahr"
                     /locus_tag="ACHY09_002943"
     CDS             5186..6205
                     /gene="ahr"
                     /locus_tag="ACHY09_002943"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFS3885891.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTILNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            6335..7837
                     /gene="yjgR"
                     /locus_tag="ACHY09_002944"
     CDS             6335..7837
                     /gene="yjgR"
                     /locus_tag="ACHY09_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFS3885892.1"
                     /translation="MSEPLLIARTSDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLPWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(7956..9038)
                     /gene="lptG"
                     /locus_tag="ACHY09_002945"
     CDS             complement(7956..9038)
                     /gene="lptG"
                     /locus_tag="ACHY09_002945"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFS3885893.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9038..10138)
                     /gene="lptF"
                     /locus_tag="ACHY09_002946"
     CDS             complement(9038..10138)
                     /gene="lptF"
                     /locus_tag="ACHY09_002946"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFS3885894.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLL
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10201..10251)
                     /gene="ytgA"
                     /locus_tag="ACHY09_002947"
     CDS             complement(10201..10251)
                     /gene="ytgA"
                     /locus_tag="ACHY09_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFS3885895.1"
                     /translation="MSIIILATISTTQENK"
     gene            10405..11916
                     /gene="pepA"
                     /locus_tag="ACHY09_002948"
     CDS             10405..11916
                     /gene="pepA"
                     /locus_tag="ACHY09_002948"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFS3885896.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYRGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12050..12493
                     /gene="holC"
                     /locus_tag="ACHY09_002949"
     CDS             12050..12493
                     /gene="holC"
                     /locus_tag="ACHY09_002949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFS3885897.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            12493..15348
                     /gene="valS"
                     /locus_tag="ACHY09_002950"
     CDS             12493..15348
                     /gene="valS"
                     /locus_tag="ACHY09_002950"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS3885898.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLSADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(15402..>16555)
                     /gene="yjgN"
                     /locus_tag="ACHY09_002951"
     CDS             complement(15402..>16555)
                     /gene="yjgN"
                     /locus_tag="ACHY09_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFS3885899.1"
                     /translation="VFHGTGERYFLICVVNVLLTIITLGIYLPWALMKCKRYLYANME
                     VNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCVLTLLLLVFLIFMAAK
                     GLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLPANSSSS
                     VIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYAILA
                     FLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIA
                     VSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLL
                     KIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVMPSLPFL"
BASE COUNT         4240 a         4291 c         4189 g         3835 t
ORIGIN      
        1 gatggcagaa cgcgccattt tacaagcatt ttccataaca gatataagac tgattgagcg
       61 gttggattaa gcatgacgat atgtttaaaa aaaccagttt tatcgacata acgccaaaat
      121 atgtttacag catcgacata tcaacgccat atgtacattt tttggcattt tttaaacata
      181 tcatcagcag gctttatttt ttgctatcga aatctacagt ccctcgtttc aatacaaaaa
      241 ttccagcgtg tcagctacct tttctgggtt tctttattgg ttttagcagt gcagcaagag
      301 atgcagatgt cacgacttct gttttaactt cagaatcgat tgatttttct tgtgatgtcg
      361 agaattgctt gtcgaaggca tcgtttttta tcacgtttcc ggtatgcctg tttgtgtgag
      421 agatagcagc aggacgagaa acacgcgttt tatttattag ccgggaatcc tgaagttggc
      481 gtccctgttc atcctcagcg gcaagtttgt tcagatcctt ttcaagaccc aaaatctgca
      541 taactgacaa ataagcgccg attgtcacgc ccgtcccgcc tgattcaaga gaacgcaagg
      601 tcattggcga catcccggct cgctcagcaa cccgcttcgc tgtgagctta cgtcgcagtc
      661 gcgccagctt aagccgttcg ccaaactcag caagcaactg attagtaccg ggaactaagg
      721 gagccgtttt tttagccata gtgataatat tttatctttt caatacctat atagccataa
      781 tattagcgca tcatgccgaa ggtgcggtgc gccgtggaag gtggaacatc aagtttacct
      841 gcgttctcca ctatctttga tgggaaccag caagcctttg accttcagtt tgatgatgtc
      901 gcgactgact ctgggctggc attattgata acataagcgc atcttaatcg ggcagttggc
      961 gttacgccct gccattgttc acaataatac cgttcatgat tcatgaacaa aactctgttg
     1021 tgaacatttc atctaaaact acatcctcaa ccatcacttg ccataccatc ccagaaatca
     1081 ttgatgatct gctcaatccc agcatcaccc acgtcatgtt gtcgtactat cacatcgatc
     1141 aggcgttctt tatcaagatg gagtaaatcc tgcaaggatg agtgttcact aattgtcgct
     1201 atatcgcttc ccaacagaaa agcgcccata cgtatgggat cccagttcag gcgttcccct
     1261 tcgacgggcg attcatagat gcgttcattc tcgataacac catattcaca gataatctta
     1321 aagagatcgg tcgcgtcttt agtactccca tgcccacgat cacgccatgc aaaaagtttt
     1381 aacagagcca gtcctggcaa ggagcagaac ggaatatttt cgctgttatc cagccgcaca
     1441 actacggtat gagaaaagac ttcggcaaat ccatccacag ccattatgat ctcacgttct
     1501 ggtggccagg caatttgatt tccttcagca acaccaccaa aaggaataat atctagctcg
     1561 atccctttga caatcatacg atgggcatta ccttggacaa cttctgcccc ttcagcaagg
     1621 aaagcatttt taagcgcatc aaatctcggc cagctatcca caaagacggc aatatcaata
     1681 tcacgggttc ggcgaccaat acttctgctg tgtatatgat ggagcagaat ttcacgagcc
     1741 gttgcacctg caataaaaaa cgggatcccc tgagcggtgc aaatacggtt gatctgactc
     1801 agtaacgttt cagtagctga agagatcgga ttcattaagg tgaagatatt tgtcattgat
     1861 tatccttgct gtttcccagt ttctgtcatc cccgcttgcc agcagttcag ccacgcatag
     1921 catcgcctcg gctttgtgat tcagtacaaa gttcccccaa aaaccggcga tcagctgtag
     1981 tttccccccc ggaacaggtt ttagtcccag ttcctttcgc ctttgtaaca acggatggga
     2041 tgtaaaaatc acgccatttt ctggtatcag gtatcctccg ctcagctttg ccgccgcaat
     2101 ctcgccgctc cagtattccc cggacgcaag cgtcaactca tcccatgaaa caggcgcgga
     2161 taaagaaagc gaatccagct tagggcgcaa agctgtagca taatctttca gccacaaagc
     2221 ctctaactcg ccttcattga ttaagcgacg ccccttcttc gatttgcgga aatagcgttg
     2281 cgcttcgaga taatcaaaag ccttgctgac cattcccaga gagatacctg cctttccggc
     2341 cagactgcgg taagtctcgt tcagggtctc tgggctagac agcaaaacaa aaagaagctt
     2401 catcacccct tcagcaaaac gactgctggc ggaaacgggt ttcttttcat agcggccttc
     2461 gatccgcaca tacaagcctg gaacctggat acgggcattc cctgccgtat cgataaagtt
     2521 gatctggctg tcggcgcaat attccgccag tgcagacgtc aggcggttac acaccagcag
     2581 cacaggaaaa tccgcaggaa agcgaaccat ttgttcgcga acagccatca gtgactcttt
     2641 acggtggata aattttactt ccagagcaaa tatcactgac tcaccatccg gccctgttag
     2701 cttaccccag gcatcgtgct taccatgtct accgctatgt tcatactgca aatggaatcc
     2761 ttttggcaga ttctctatcg catcagacaa aagctgttct tccttcattt gttcacacct
     2821 cataactgtt catgaatcat gaacaaaaca atgttatgaa caaataaaga aagaagcaac
     2881 gtaaatgatc aaaaaaagac cgccagaaat gcgatgcctt attcaacctt ccaaacgtca
     2941 ttttccatta aagaaagaag ggtaaagagt agttggacgc attctttcag ggttgagtaa
     3001 ccattcctac tcccccgact ccacctcctc ctgcgccgcc atcagaacca actctgactt
     3061 cgaaatcagc aggtctttcg gcctcccacc tctcaacctg cgattagccg agccaaacca
     3121 gatagccagc ccccacggtg ttttacgctc cttaaacagt gaaagaatca aacggacaac
     3181 cttgagaggc cgcattttct catccagaac ataatcaggg tataaatcct caccatcgac
     3241 cttcagcgag aatatcttcc cggcggcttt ccagcctttc ggaccagcat caggatcggc
     3301 ggaacgaaag cccgcttttg cggataattc agacgccgtc agccattccg agtcagccag
     3361 tattctggct ttcagctcct ctagctgccg gtcattgcgc tcctggaaac tcatatggtc
     3421 gagtgcgcct cccggcgact ttttgaagcc atacggcaca acatgcctga accggtttgc
     3481 atccagataa tccgcccacc actgcatcat ctcccaacgt tcttcgagat gctgcgcctt
     3541 gtggatatac gccgcacgca cgcgcttgcg ctcctgatgg ctcatctggc gctctaccgc
     3601 gtcgcttgac cacagccccg actccaccag cgcgctacag gccatagtgc gaaaaccgtg
     3661 gccacagaca tctttctggg tgtcgtagcc catcacccgc agcgctttgt tgatggtgtt
     3721 ttcactcatc ggcttgcggt aattgtgatc gccggggaac accagttcag ttcccggtcg
     3781 gcaatgctgc cgcacctcgt gcagcagttc gatggcctga cgtgacaatg ggaccaggtg
     3841 tggggagtgc atcttcgccc cacgcgctga atatttaacg ccggggatcg gctcacgctc
     3901 agcggggatg gtccacatgg cgttacgcag atcaatttca tcccagcggg caaagcgcag
     3961 ttcgctggag cggataaaca gcagcagtgc cagttttacc gccagttgag tcagttgacg
     4021 gcctttgtaa tcgtcgattc tttccaggaa atcagggata agattcagat ccaacgccgg
     4081 gtgatgccgt actttgggcg tggagaccgc gccggttaaa tctgatgcag ggttatgatc
     4141 gataatgccg ttctggacgg cgtagcgcat cacgcaggca gtgcgctgct gaagccggga
     4201 agcaacatcc agtttgcccg ctttctccac ttccttgatg ggcaccagca gatctttgac
     4261 cttcattttg gtgatgtcac agctgccgtt cgtggggaat acgtaaagct cgaaataacg
     4321 cagcacccgc ttcttatgtt ccgccgacca gttcacgtta ctggtgaccc attcacgtgc
     4381 cacagactca aacgtatgtt ccggcgttat cgcaaggcgt tctgcttttc ggcgtgcact
     4441 cggatcgatg ccggaaatca gcaataattg tgcctcttca cgcatgcgtc ttgcctgtgc
     4501 aagcgtcaaa agcggataag ggcctaaagc gagctttttc tctttgttta caaagcggta
     4561 cttgatgtac cagcgttttg aaccgttagg gttaaccagc aggtaaagtc catgcgagtc
     4621 actgagctta tacggcttgc cacaaggctt ggcgtggact atagcgcgat ctgttagtgc
     4681 catttggggg cctcactatt atcgaactac gtctaagccc ccaatttggc ccccaaatcg
     4741 cgtggatgtc aatggatgaa aaatgaccaa cacggaaaga ccacaaaata aatatggtta
     4801 ttgtggaaaa ataaattacc tttgaattac aaacggatat ggacgaattt ggacggaaat
     4861 ggacacaaaa aaagccaccc ttaggtagct aattattgca tcacttggtg ccgaaggccg
     4921 gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct accgatttcg
     4981 ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt cgcacgccac
     5041 catgcaagcg ccagcttgct cgcacctcgc taagtgctga aattttcagc attgcatacc
     5101 gcgatgtgta acctttgtca cactccaggc gccccgccct gccatgctct acacttccca
     5161 gaccacacca gagaaggata aaaacatgtc gatgataaaa agttatgccg caaaagaggc
     5221 tggcggcgaa ctggaagttt atgagtacga tcccggtgaa ctgaagccac aagatgttga
     5281 agttcaggtg gattactgcg ggatctgcca ttccgatctg tcgatgatcg acaacgaatg
     5341 gggattttca cagtatccgc tggttgccgg gcatgaggtg attgggcgcg tggttgcatt
     5401 gggtagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga ttggctggac
     5461 ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca actgcgagca
     5521 aggtgcggtg ccgacgattt tgaatcgcgg cggttttgcc gagaagttgc gtgcggactg
     5581 gcaatgggta atcccactgc cagaaaatat cgacattgaa tctgctgggc cgctgttgtg
     5641 cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcca ccagccgcgt
     5701 cggcgtgatt ggtattggcg ggctggggca tatcgctata aaacttctgc acgcaatggg
     5761 atgcgaggtg acagccttta gttctaatcc ggcgaaagag caggaagtac tggcgatggg
     5821 tgccgataaa gtggtgaata gccgcgatcc gcaggcactc aaagcactgg cggggcagtt
     5881 tgatctcatt atcaacaccg tcaacgtcag cctcgactgg cagccttatt ttgaggcgct
     5941 gacgtatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt ctgttccggc
     6001 ctttacgtta attgcgggcg accgcagcgt ctctggttct gctaccggca cgccttatga
     6061 gctgcgtaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacta ccgaactgtt
     6121 cccgatgtcg aaaattaacg acgctatcca gcatgtacgc gatggtaaag cgcgctaccg
     6181 cgtggttctg aaagccgact tctgatccct ttgccgggtg cctgtcgccc ggcgtttaaa
     6241 tctctttgga aggcgtctcg caatctgctg aatttagcgg tgaagagtgc gcagccgttg
     6301 cctatactcc aggcaggaaa ctggaggata tctcatgagc gaacccctgt taattgcccg
     6361 cacgtcggac acagaactgt ttttattgcc gggaatggcg aatcgtcacg ggctgattac
     6421 tggcgcaacg gggacgggta aaaccgttac gctgcaaaaa ctggcagagt ctctatctga
     6481 aatcggcgtg ccggtgttta tggccgatgt gaaaggtgat ttaacgggag tagcggagga
     6541 aggtacttct tcggaaaaac tgctcgcaag gcttaaaaat atcggcgtaa atgactggca
     6601 acctcatgct aatccggtgg tggtgtggga tatctttggc gagaaaggcc acccggtgcg
     6661 ggcgacggtt tcggatctgg ggccgctgtt gctggcgcga ctgttgaatc tcaacgatgt
     6721 gcaatctggc gtgctgaata ttatcttccg cattgcggac gatcagggat tgttgctgct
     6781 cgactttaaa gatttgcggg cgattaccca gtacatcggc gataacgcca aatccttcca
     6841 gaatcagtac ggaaatatca gtagcgcatc ggttggtgcc atccagcgcg gattactgtc
     6901 gctggaacag caaggcgcag cacacttctt tggtgagccg atgctggata tcaaagactg
     6961 gatgcgcacc gatgccaacg gtaaaggcgt tatcaatatc ctcagcgccg agaaacttta
     7021 tcagatgccg aaactgtacg ccgccagcct gccgtggatg ctttcagagt tatatgaaca
     7081 attgcctgaa gcgggcgatc tggagaaacc aaaactggtg tttttcttcg acgaagcaca
     7141 tctgctgttt aacgatgcgc ctcaggtact gctggataag attgagcagg tgataaggct
     7201 tattcgctca aaaggcgtgg gcgtctggtt cgtttcgcaa aacccgtctg atattccgga
     7261 taatgtgctc gggcagctcg gtaatcgtgt tcaacacgct ttgcgggctt ttacgcccaa
     7321 agatcagaaa gcagtaaaag ctgcggcgca aaccatgcgg gccaatccgg catttgatac
     7381 cgaaaaggca atccaggaac tggggaccgg cgaggcgtta atctcgtttc tcgatgcaaa
     7441 aggaagtcct tctgtggtgg aacgggcgat ggtgatcgca ccttgttcgc gaatggggcc
     7501 ggtgacggaa gatgagcgta atggcctgat taatcactcc ccggtgtatg gcaaatatga
     7561 agatgaggtg gaccgcgagt ccgcctatga gatgctgcaa aaaggctttc aggccagtac
     7621 cgagcagcaa aataatcccc ccgtgaaagg gaaagaggtg gcggtggatg acggtattct
     7681 tggtggattg aaggatattt tgtttggcac taccggacca cgcggcggga agaaagatgg
     7741 tgtggtgcaa acaatggcga aaagcgccgc tcgccaggtg acgaatcaga ttgtacgcgg
     7801 gatgttgggg agtttgctgg gggggagaaa aagataagtc agacgtgatc tgtgcccctc
     7861 accctaaccc tctccccaaa ggggcgaggg gaccgttcga actcgtattt gactttgtca
     7921 acaataataa agggagcttt cgctcccttg ttagattacg attttctcat taacagccac
     7981 aggctgatta agaagaagct ggcgcttggc aacagtgcgc cgatgatcgg cgggatgcca
     8041 taaaccaacg tcagcgggcc gaagatctgg tccagtacgt agaagacaaa accgaagctg
     8101 ataccggtga ccacacgcac gcccatcggt acgctacgca gtgggccaaa gatgaacgac
     8161 agcgccatca gcatcatcac cgccacggat agcggctgga agattttgct ccacatgttg
     8221 agctgataac gtccggcatc ctgaccgctc gacttcagat acttcacgta gttgtgcaaa
     8281 ccactgatgg agagtgcatc cgggtccagc gccaccacgc ccagtttgtc tggcgtgagg
     8341 ttggttttcc aggtgccgct caccgtctgc gaaccggtaa tctgtttcgg attggtcagg
     8401 tcagattcat caacctgcga cagacgccag actttatgtt ccgggtcaaa cttcgcggta
     8461 gcggcataac gtacggattg cagacgacga ttctcgttaa aggcataaat actgatgcca
     8521 cctaactcct cgtcaccttt aacccgctca atgtagacga agttgttgcc atctttcgcc
     8581 cataagcctt gctgggtaga gagcaacgag ccgccgtaca tcgcctgcgc acggtagtta
     8641 cgcgccatct gctcgccctg cggcgcgacc cattcgccaa tcgccatcgt cagcaagacc
     8701 agtggaatgg cggttttcat caccgacagc gctacctgca tacgggtaaa accagaagcc
     8761 tgcatcacca ccagttcgct gcgctgcgcc agcatcccaa gaccaagcaa cgccccaagc
     8821 agagccgcca tcgggaagaa gatttgcaca tctttcggca cgctcagcaa ggtatacatt
     8881 cctgcgccta acgcgtcgta actcccctgc ccggcttttt tcagctgatc gacaaacttg
     8941 ataatgcccg acagcgacac cagcatgaac agcgtcatca tgatggtggt gaaaatagtt
     9001 ttaccgatat agcggtcaag tacgccaaaa ggttgcatca caccgctcct ttacgcgaaa
     9061 aactggcgcg caggcggcgg accggcacgg tgtcccaaag gttgagaaca atcgccaaag
     9121 ccagataaat caggttaacg gtccacatcc acagcgtcgg gtccagctta cctttaccgc
     9181 cgttcgattt cagggaggtc tggatcagga agaaaagtag atacagcagc atggctggca
     9241 gcatcgacag tacgcgtccc tgacgcgggt taaccacgct cagcggtacg accataagtg
     9301 ccatcataaa cacggtgaat accaacgtga tacgccagtt cagttctgca cgggcacgat
     9361 cggtgtcagt gttccacaat gtgcgcatgt ccatctggtc ggtatcgttc gggtcgagcg
     9421 ccaccgcctg gtgaccaatg atcgcctgat aatcctggaa gtccgtaatg cggaaatcac
     9481 gtaacagtgc agtgccttcg aagcgcgttc cctggttgag agtgacgacc tgggagccgt
     9541 cgcgcagctg ggttaaatgt ccggaatcgg ccaccaccac agaaggacgt gcattacctt
     9601 ttggtcgaat ttgcgcgagg aacacatctt tgaaatcgct gccgtcaacg ctttcgatta
     9661 acagcaccga gctgccatta gtcgcttgct ggaattgccc ttgcgccagc gccgccatgc
     9721 cagggttcgc tttcgcttct gctaacactt catcctgatg acgcgatgac cacggtcccg
     9781 cccacatcac gttaaccgcc gcgacgattg ccgtgaatac cgcaaggatc attgccgctt
     9841 tcaccagaac cgctttgctc aggccgcagg catgcattac cgtaatttca ctttcggtat
     9901 acagtttgcc cagcgtcatc agcagcccga ggaacaggct taatggcagg ataagctgcg
     9961 ccatttccgg cacgcccaac ccgagaaggg agagcaccag attcgccgga atatcgccgt
    10021 caaccgctgc gccgaggatc ctcactaact tttgacagaa gaagatcaaa agcaagatga
    10081 agagtatcgc cagctggctt ttgagcgtct cccgcaccag atatcttatg attatcactt
    10141 taaatacgcc cgtaaaaact cgtcttttgc aggattttag cttgtttcat ggcttaaacg
    10201 tcatttattc tcttgagtcg tcgaaatcgt cgctaagata attatactca acggattcac
    10261 ctctcagatt ttgttctgac gtgccaatgc cgtaataacg ttaagattaa cacgaagtca
    10321 tcgcaacagc ggacatgagt tacgaaagct tgcaattcta tctgtagcca ccgccgttgt
    10381 ctttaagatt caggagcgta gtgcatggag tttagtgtaa aaagcggtag cccggagaaa
    10441 cagcggagtg cctgcatcgt cgtgggcgtc ttcgaaccac gtcgcctttc tccgattgca
    10501 gaacagctcg ataaaatcag cgatgggtac atcagcgccc tgctacgtcg gggcgaactg
    10561 gaaggaaaac cggggcagac actgttgctg caccatgttc cgaatgtact ttccgagcga
    10621 attctcctta ttggttgcgg caaagaacgt gagctggatg agcgtcagta caagcaggtt
    10681 attcagaaaa ccattaatac gctgaatgat actggctcga tggaagcggt ctgctttctg
    10741 actgaactgc acgttaaagg ccgtaacaac tactggaaag tgcgtcaggc tgtcgagaca
    10801 gcaaaagaga cgctctacag tttcgatcag ttgaaaacga ataagagcga accgcgtcgc
    10861 ccgctgcgta agatggtgtt caacgtgccg acccgccgtg aactgaccag cggtgagcgc
    10921 gcgatccagc acggtctggc gattgccgcc gggataaaag cagcgaaaga tctcggtaat
    10981 atgccgccga atatctgtaa cgccgcttat ctcgcctctc aggcgcgcca gctggctgac
    11041 agctacagca agaatgtcat cacccgcgtt atcggcgaac agcagatgaa agagctggga
    11101 atgcactctt atctggcggt cggtcagggt tcacaaaacg aatcgctgat gtcggtgatt
    11161 gagtacagag gcaacgcgtc ggaagatgca cgcccaatcg tgctggtggg taaaggttta
    11221 accttcgact ccggcggtat ctccatcaag ccttcagaag gcatggatga gatgaagtac
    11281 gatatgtgcg gcgcggcggc ggtttacggc gtgatgcgta tggtcgcgga gctgcaactg
    11341 ccgattaacg ttattggcgt gctggcaggc tgtgaaaaca tgcctggcgg gcgtgcctat
    11401 cgtccgggcg atgtgttaac caccatgtcc ggtcaaaccg ttgaagtgct gaacaccgat
    11461 gccgaaggcc gcctggtact gtgcgacgtg ttaacttacg ttgaacgttt tgagccggaa
    11521 gcagtgattg acgtggcgac gctgaccggt gcctgcgtga tcgcgctggg tcatcatatt
    11581 actggtctga tggcgaacca taatccgctg gcccatgaac tgattgccgc gtctgaacaa
    11641 tccggtgacc gcgcatggcg cttaccgctg ggtgacgagt atcaggaaca actggagtcc
    11701 aattttgccg atatggcgaa cattggcggt cgtcctggtg gggcgattac cgcaggttgc
    11761 ttcctgtcac gctttacccg taagtataac tgggctcacc tggatattgc aggaaccgcc
    11821 tggcgttctg gtaaagcaaa aggcgcaacc ggtcgtccgg tagcgttgct ggcacagttc
    11881 ctgttaaacc gcgctgggtt taacggcgaa gagtaagttt gcgttttacc ctcaccctaa
    11941 ccctctccct aagggagagg gaaccgatcg agcgcaatag aaattcattg ctcatctttc
    12001 agggtgagga atgcagcaat atttaaatcc accacaagaa gccccatata tgaaaaacgc
    12061 aacgttctac cttctggaca atgacaccac cgtcgatggc ttaagcgccg ttgagcaact
    12121 ggtgtgtgaa attgccgcag aacgttggcg cagcggtaag cgcgtactca tcgcctgtga
    12181 agatgaaaag caggcttacc ggctggatga agccctgtgg gcgcgtccgg cagaaagctt
    12241 tgttccgcat aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg agatcgcctg
    12301 gccgcaaaag cgtagcagca gcccgcgcga tatattgatt agtctgcgaa caagctttgc
    12361 agattttgcc accgctttca cagaagtggt agacttcgtt ccttatgaag attctctgaa
    12421 acaactggcg cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc tgaatacggc
    12481 aacctggaaa taatggaaaa gacatataac ccacaagata tcgaacagcc gctttacgag
    12541 cactgggaaa agcagggcta ctttaagcct aatggcgatg aaagccagga aagtttctgc
    12601 atcatgatcc cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc cttccagcaa
    12661 accatcatgg ataccatgat ccgctatcag cgcatgcagg gcaaaaacac cctgtggcag
    12721 gtcggtactg accacgccgg gatcgctacc cagatggtcg ttgagcgcaa gattgccgca
    12781 gaagaaggta aaacccgtca cgactacggc cgcgaagctt tcatcgacaa aatctgggaa
    12841 tggaaagcgg aatctggcgg caccattacc cgtcagatgc gccgtctcgg caactccgta
    12901 gactgggagc gtgaacgctt cactatggac gaaggcctgt ccaatgcggt gaaagaagtt
    12961 ttcgttcgtc tgtataaaga agacctgatt taccgtggca aacgcctggt aaactgggac
    13021 ccgaaactgc gtaccgctat ctctgacctg gaagtggaaa accgcgaatc gaaaggttcg
    13081 atgtggcaca tccgctatcc gctggctgac ggcgcgaaaa ccgcagatgg taaagattat
    13141 ctggtggttg cgactacccg tccggaaacc ctgctgggcg ataccggcgt ggccgttaac
    13201 ccggaagatc cgcgttacaa agatctgatt ggcaaatatg tcattctgcc gctggttaac
    13261 cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac cggctgcgtg
    13321 aagatcaccc cggcgcacga ctttaacgac tatgaagtgg gtaaacgtca cgccctgccg
    13381 atgatcaaca tcctgacctt tgacggtgat atccgtgaaa gcgcccaggt gttcgatacc
    13441 aaaggtaacg aatctgacgt ttattccagc gaaatcccgg cagagttcca gaaactcgag
    13501 cgttttgccg cacgtaaagc ggtcgttgct gcggttgacg cactcggcct gctggaagaa
    13561 attaaaccgc acgacctgac cgttccttac ggcgaccgtg gcggcgtagt tatcgaaccg
    13621 atgctgaccg accagtggta cgtgcgtgcc gatgtcctgg cgaaaccggc ggttgaagcg
    13681 gttgagaacg gcgacattca gttcgtaccg aagcagtacg aaaacatgta cttctcctgg
    13741 atgcgcgata ttcaggactg gtgtatctct cgtcagctgt ggtggggtca ccgtatcccg
    13801 gcatggtacg acgaagcggg taacgtttat gttggccgca acgaagacga agtgcgtaaa
    13861 gaaaataacc tgagcgcgga cgtcgcgctg cgtcaggacg aagacgttct cgacacctgg
    13921 ttctcctccg cgctgtggac cttctccacg ctcggctggc cggaaaacac cgacgcactg
    13981 cgccagttcc acccaaccag cgtgatggta tccggcttcg acatcatctt cttctggatt
    14041 gcccgcatga tcatgatgac catgcacttc atcaaagatg aaaatggcaa accgcaggtg
    14101 ccgttccaca ccgtttacat gaccggcctg attcgtgatg acgaaggcca gaagatgtcc
    14161 aaatccaagg gtaacgtgat tgacccgctg gatatggttg acggtatttc gctaccggaa
    14221 ctgctggaaa aacgtaccgg caatatgatg cagccgcagc tggcggacaa aatccgtaag
    14281 cgcaccgaga agcagttccc gaacggcatt gagccgcacg gtactgacgc gctgcgcttc
    14341 accctggcgg cgctggcttc taccggtcgt gacatcaact gggatatgaa gcgtctggaa
    14401 ggctaccgta acttctgtaa caagctgtgg aacgccagcc gctttgtgct gatgaacaca
    14461 gaaggtcagg attgcggctt caacggcggc gaaatgacgc tatcgctggc ggaccgctgg
    14521 attctggcgg agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga cagcttccgc
    14581 ttcgatatcg ccgcaggcat tttgtatgag ttcacctgga accagttctg tgactggtat
    14641 ctggagctga ccaagccggt aatgaacggt ggaacagaag ccgaactgcg cggtactcgc
    14701 catacgctgg tgactgtact ggaaggtctg ctgcgcctcg cgcatccgat cattccgttc
    14761 atcaccgaaa ccatctggca gcgtgtgaaa gtactttgcg gtatcactgc cgacaccatc
    14821 atgctgcagc cattcccgca gtatgatgca tctcaggttg atgaagccgc actggccgac
    14881 accgagtggc tgaaacaggc gatcgttgct gtacgtaaca tccgtgcaga aatgaacatc
    14941 gcgccgggca aaccgctgga gctgttgctt cgtggttgca gcgcggatgc agagcgtcgc
    15001 gtaaatgaaa accgtggctt cctgcaaacg ctggcgcgtc tggaaagcat caccgtgctg
    15061 cctgccgatg acaaaggtcc ggtttccgtt accaagatca tcgacggcgc agagctgctg
    15121 atcccgatgg ctggcctcat caacaaagaa gatgagctgg cgcgcctggc gaaagaagtg
    15181 gcgaagatcg aaggtgaaat cagccgcatc gagaacaagc tggcaaatga aggctttgtc
    15241 gcccgcgcac cggaagcggt tatcgcgaaa gagcgtgaga agctggaagg ttatgcagaa
    15301 gcgaaagcca agctgattga acagcaggct gttatcgccg cactgtaatc aacacagtga
    15361 agaaaacgaa ggccggagca tgctccggcc ttttttatct cttacagaaa tggtaaagaa
    15421 ggcataactc cccgagaaat actggctaag aagcctttat ctggttgttc ttctttattg
    15481 attaaaggaa ggtcatccaa atcgcccagc aaatacgtat tttttgcctg ccagtcaatc
    15541 atccatattt tcagcagtgg ataagccaga ccgcccgtaa tcccggatat caccaccaac
    15601 gcacacatgc gataaagcat accgtggtac gttaaagttg agcgaaaacg aatacgccca
    15661 tcattcagtg acaggttgct cataaaatga tttcgcaacg acaccgttaa ataactggtg
    15721 ctaacagcaa tcccaaaata atagattaac tgcgcgatta tcattttacg ttgcatttcc
    15781 ataaattgct gtaaattctc aatgtcatca tttgcatata cagaactatc atacgcattt
    15841 aatatttgat cgaagataat ataaccagca acagcgagaa aaggcaataa agctaaaaat
    15901 gcgagaatgg catattttat acaatacgta gtatctaatt tcaccttgaa acgatgtata
    15961 ccgaaactgg tattgctcca gagaaagctc attaccaggc tatataaagt accattaaaa
    16021 ataccaatgg aaacaatacc aactattgcc atcagaacca cagatataat aacactactt
    16081 gaactattgg caggtagcat ctttgtcgag ataaagaaaa cagtccccat cccaatggcc
    16141 attaaaatcg gcaagaaaaa ggttacccac cagaaccctt tcaaagagca attaaaacta
    16201 aatcttacgc cgttgagact ggtcattaag gcctgataac gcagtccttt tgctgccata
    16261 aatataagaa aaaccaacag taacaaagtc aaaacacagc caacaagcgg catatctgct
    16321 gacactgtca ttaagattgc gaaatagaaa aaaacaaaaa caagacaact aacaaaaaca
    16381 ttcccaccgg taattccata agaaaatcgt tgtccgttaa cttccatatt agcataaaga
    16441 taacgcttac atttcattaa tgcccatggt aaatagatac ctagcgttat aatcgttaac
    16501 aacacattca ccacacaaat aagaaaatat ctctcacctg taccatgaaa aacaa
//
LOCUS       DAZUCC010000109        16485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZUCC010000109 DAZUCC010000000
VERSION     DAZUCC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.109"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..790)
                     /gene="espL1"
                     /locus_tag="ACHY09_002952"
     CDS             complement(<1..790)
                     /gene="espL1"
                     /locus_tag="ACHY09_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFS3885900.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFACQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTTENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRSLL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTL"
     gene            1313..3241
                     /gene="thrS"
                     /locus_tag="ACHY09_002953"
     CDS             1313..3241
                     /gene="thrS"
                     /locus_tag="ACHY09_002953"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS3885901.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            3245..3787
                     /gene="infC"
                     /locus_tag="ACHY09_002954"
     CDS             3245..3787
                     /gene="infC"
                     /locus_tag="ACHY09_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS3885902.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3884..4081
                     /gene="rpmI"
                     /locus_tag="ACHY09_002955"
     CDS             3884..4081
                     /gene="rpmI"
                     /locus_tag="ACHY09_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS3885903.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            4135..4491
                     /gene="rplT"
                     /locus_tag="ACHY09_002956"
     CDS             4135..4491
                     /gene="rplT"
                     /locus_tag="ACHY09_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS3885904.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4608..4732
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4614..4658
                     /gene="pheM"
                     /locus_tag="ACHY09_002957"
     CDS             4614..4658
                     /gene="pheM"
                     /locus_tag="ACHY09_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFS3885905.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4942..5925
                     /gene="pheS"
                     /locus_tag="ACHY09_002958"
     CDS             4942..5925
                     /gene="pheS"
                     /locus_tag="ACHY09_002958"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS3885906.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5940..8327
                     /gene="pheT"
                     /locus_tag="ACHY09_002959"
     CDS             5940..8327
                     /gene="pheT"
                     /locus_tag="ACHY09_002959"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS3885907.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVIECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGAAIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8332..8631
                     /gene="ihfA"
                     /locus_tag="ACHY09_002960"
     CDS             8332..8631
                     /gene="ihfA"
                     /locus_tag="ACHY09_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFS3885908.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8732..9712
                     /gene="btuC"
                     /locus_tag="ACHY09_002961"
     CDS             8732..9712
                     /gene="btuC"
                     /locus_tag="ACHY09_002961"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFS3885909.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9775..10326
                     /gene="btuE"
                     /locus_tag="ACHY09_002962"
     CDS             9775..10326
                     /gene="btuE"
                     /locus_tag="ACHY09_002962"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFS3885910.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            10326..11075
                     /gene="btuD"
                     /locus_tag="ACHY09_002963"
     CDS             10326..11075
                     /gene="btuD"
                     /locus_tag="ACHY09_002963"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFS3885911.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQHQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLFLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            11154..11618
                     /gene="nlpC"
                     /locus_tag="ACHY09_002964"
     CDS             11154..11618
                     /gene="nlpC"
                     /locus_tag="ACHY09_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS3885912.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11865..12578
                     /gene="ydiV"
                     /locus_tag="ACHY09_002965"
     CDS             11865..12578
                     /gene="ydiV"
                     /locus_tag="ACHY09_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFS3885913.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPVNQITTLVQR"
     gene            12641..14077
                     /gene="selO"
                     /locus_tag="ACHY09_002966"
     CDS             12641..14077
                     /gene="selO"
                     /locus_tag="ACHY09_002966"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFS3885914.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVALSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYASRPPDWGKRLEVSCSS"
     gene            complement(14081..14272)
                     /gene="hemP"
                     /locus_tag="ACHY09_002967"
     CDS             complement(14081..14272)
                     /gene="hemP"
                     /locus_tag="ACHY09_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFS3885915.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(14404..15450)
                     /gene="aroH"
                     /locus_tag="ACHY09_002968"
     CDS             complement(14404..15450)
                     /gene="aroH"
                     /locus_tag="ACHY09_002968"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFS3885916.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15607..16440)
                     /gene="ppsR"
                     /locus_tag="ACHY09_002969"
     CDS             complement(15607..16440)
                     /gene="ppsR"
                     /locus_tag="ACHY09_002969"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFS3885917.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         4136 a         3841 c         4368 g         4140 t
ORIGIN      
        1 acaaagtatg attgtctata tataaaatcg cggacatatg actgacattg tttgccatca
       61 ttcgacagaa gaaaatatct aaagcgaagc caaaatcctt actcgcgata acgcgactcc
      121 ctgcaggagg atggcgaagt aatgatctga actccgcgtc ccggactaag ggaacatgct
      181 gctgtatgga ttcaacggag gagtaatgtt taatcagatt gactttgcct tttccatttc
      241 tcagtgaatc aagggcgtat tgcaatgaca gatggcgaca aaaaatagcg tcagaaggag
      301 attgcgaaaa aaatgcacta ccattgagat ttactgttgg ttgtatagtt ttttctctat
      361 ggattttttt agacagtttc atacaatggt tacgattttc ggtagttaac tgttcctgtt
      421 caatataatc cagtatgctt cgcaaatggt tagctttaat aggcgtggtt tccagtaata
      481 aggaaatgat ttctttttta tagtgtaatg taatgagtat attttctgca tggatgggtg
      541 aagtggcaaa gttaatggta aaaagggcag tggaataaca attttgtgat atttttcgac
      601 agtttgcggt aaattcctga gtaacacagt atgacagcat tgaacttaag tcagaggtat
      661 caagtagggt gatgagttgg cacgcaaatt gattaatagg ctctgatgaa ggcgttggta
      721 tgtcgctatc gatgtgcatt ctatgaggag acttataatg agttctgata ataatgtcgt
      781 tttgactcat aatctattcg ccttatatat gtctggaggt tttgaggatg atgattaatt
      841 attatcgcca gaacgaaatg tgcacgaaaa attgctataa gaattgtgtc acttttatca
      901 ttgtgatatg accacttcgt cattaatctt atgatcgttt aaatagtacc gccaaattac
      961 gcaacgaact ttgctggatt cagtttatgc tgctgcaaat ccgctcgcag taaacctttc
     1021 agacgctccg tgatgttatc agtggttctt taagcgtttt gctggtgtac tcactacaaa
     1081 cgaattgcga atcgaatcaa tgtgaaacgg aaaggtacaa tctccctttt tttagtgtta
     1141 acatcgttca accgggtgga aggtgagagg ttaatgtctt tcatttgtgt acctgattgc
     1201 gaaccaattt agcatttgtt ggctagatgg tttcgcaatg aactgttaat aaacaaattt
     1261 ttctttgtat gtgatctttc gtgtgggtca ccactgcaaa taaggatata aaatgcctgt
     1321 tataactctt cctgatggca gccaacgcca ttacgaccac gctgtaagcc ccatggatgt
     1381 tgcgctggac attggtccag gtctggcgaa agcctgtatc gcagggcgcg ttaatggcga
     1441 actggttgat gcttgcgatc tgattgaaaa cgacgcacaa ctgtcgatca ttaccgccaa
     1501 agacgaagaa ggtctggaga tcattcgtca ctcctgtgcg cacctgttag ggcacgcgat
     1561 taaacaactt tggccgcata ccaaaatggc aatcggcccg gttattgaca acggttttta
     1621 ttacgacgtt gatcttgacc gcacgttaac ccaggaagat gtcgaagcac tcgagaagcg
     1681 gatgcatgag cttgctgaga aaaactacga cgtcattaag aagaaagtca gctggcacga
     1741 agcgcgtgaa actttcgcca accgtggtga gagctacaaa gtctccattc ttgacgaaaa
     1801 catcgcccat gatgacaagc caggtctgta cttccatgaa gaatatgtcg atatgtgccg
     1861 cggtccgcac gtaccgaaca tgcgtttctg ccatcatttc aaactaatga aaacggcagg
     1921 ggcttactgg cgtggcgaca gcaacaacaa aatgttgcaa cgtatttacg gtacggcgtg
     1981 ggcagacaaa aaagcgctta acgcttacct gcagcgcctg gaagaagccg cgaaacgcga
     2041 ccaccgtaaa atcggtaaac agctcgacct gtaccatatg caggaagaag caccgggtat
     2101 ggtattctgg cacaacgatg gctggaccat cttccgtgaa ctggaagtgt ttgttcgttc
     2161 taaactgaaa gagtaccagt atcaggaagt taaaggtccg ttcatgatgg accgtgtcct
     2221 gtgggaaaaa accggtcact gggacaacta caaagatgca atgttcacca cgtcttctga
     2281 gaaccgtgaa tactgcatta agccgatgaa ctgcccgggt cacgtacaaa ttttcaacca
     2341 ggggctgaag tcgtatcgcg atttgccgct gcgtatggcc gagtttggta gctgccaccg
     2401 taacgagccg tcaggttcgc tgcatggcct gatgcgtgta cgtggtttta cccaggatga
     2461 cgcgcatatc ttctgtactg aagaacaaat tcgcgatgaa gttaacggat gtatccgttt
     2521 agtctatgat atgtacagca cttttggctt cgagaagatc gtcgtcaaac tctccactcg
     2581 tcctgaaaaa cgtattggca gcgacgaaat gtgggatcgt gctgaggcgg acctggcagt
     2641 tgcgctggaa gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg ctttctacgg
     2701 tccgaaaatt gaatttaccc tgtatgactg cctcgatcgt gcatggcagt gcggtacagt
     2761 acagctggac ttctccttgc cgtctcgtct gagcgcctcc tatgtgggcg aagacaacga
     2821 gcgtaaggta ccggtaatga ttcaccgcgc aattcttggg tcgatggaac gtttcatcgg
     2881 tatcctgacc gaagaattcg ctggtttctt cccgacctgg cttgcgccgg ttcaggttgt
     2941 tatcatgaat attaccgatt cacagtctga ttacgttaac gaattgacgc aaaaactatc
     3001 aaatgcgggc attcgtgtta aagcagactt gagaaatgag aagattggct ttaaaatccg
     3061 cgagcacact ttgcgtcgcg tcccgtatat gctggtctgt ggtgataaag aggtggaatc
     3121 aggcaaagtt gccgttcgca cccgccgtgg taaagacctg ggaagcatgg acgtaaatga
     3181 agtgatcgag aagctgcaac aagagattcg cagccgcagt cttaaacaat tggaggaata
     3241 aggtattaaa ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca atggcgaaat
     3301 tcgcgcccag gaagttcgct taacaggtct ggaaggcgag cagcttggta ttgtgagtct
     3361 gagagaagct ctggagaaag cagaagaagc cggagtagac ttagtcgaga tcagccctaa
     3421 cgccgagccg ccggtttgtc gtataatgga ttacggcaaa ttcctctatg aaaagagcaa
     3481 gtcttctaag gaacagaaga aaaagcaaaa agttatccag gttaaggaaa ttaaattccg
     3541 tcctggtaca gatgaaggcg actatcaggt aaaactccgc agcctgattc gctttctcga
     3601 agagggtgat aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg cgcaccagca
     3661 aatcggtatg gaagtgctta atcgcgtgaa agacgatttg caagaactgg cagtggtcga
     3721 atccttccca acgaagatcg aaggccgcca gatgatcatg gtgctcgctc ctaagaagaa
     3781 acagtaaggc cttcaagtag cactgtctgt ggagcctttg ggcttcacag gcattgttcg
     3841 cctttgtttc gtttattaac aatgcgaagt ggaagttatt aaaatgccaa aaattaagac
     3901 cgtacgcggt gctgctaagc gcttcaaaaa aaccggtaaa ggtggtttta agcataagca
     3961 cgctaacctg cgtcacattc tgaccaaaaa agcgaccaaa cgtaaacgtc acctgcgtcc
     4021 gaaagccatg gtttccaaag gcgatctggg cctggtaatc gcgtgcctgc cgtacgcata
     4081 agccgttaac gttttttaac tttttaatta gaatatagat acaggagagc acatatggct
     4141 cgcgtaaaac gtggtgttat tgcacgcgca cgtcacaaga aaattttgaa acaagctaaa
     4201 ggctactacg gtgcgcgttc tcgcgtatac cgcgttgcct tccaggctgt tatcaaagct
     4261 ggtcagtatg cttaccgtga ccgtcgtcaa cgtaagcgtc agttccgtca actgtggatt
     4321 gcgcgtatca acgcagcagc acgtcagaac ggtatttctt acagcaaatt catcaatggc
     4381 ctgaaaaaag cctctgttga aatcgaccgt aagatcctgg ctgatatcgc agtattcgac
     4441 aaagtagcgt tcaccgctct ggttgaaaaa gcgaaagcag ctctggcata agccagttga
     4501 aagagggagc tagtctccct cttttcgttt caacgccatc aaaacattga cttttatcgc
     4561 cgtagccttt tcaataaagg tcttttgaag agtaccaaaa ggtaacgcaa gcaatgaatg
     4621 ctgctatttt ccgcttcttt ttttacttta gcacctgact ccaggaggct agcgcgtgag
     4681 aagagaaacg gaaaacagcg cctgaaagcc tcccagtgga ggcttttttt gtatgcgcgt
     4741 tttgaaaaat aaaaagcctg tctcatcagg cgtaattgtc gcagccagtc tggacacggc
     4801 cagcgcggag aaaccggagc gtacttaagt acgtgaggat ttcgagcact gcccgggacc
     4861 aaaatggcaa gtaaaatagc ctgatgggat gggctctaag tccaacgaac cagtgtcacc
     4921 actgacacaa tgaggaaaaa catgtcacat ctcgcagaac tggttgccag tgcgaaggcg
     4981 gccattagcc aggcgtcaga tgttgccgcg ttagataatg tgcgcgtcga atatttgggt
     5041 aaaaaagggc acttaaccct tcagatgacg accctgcgtg agctgccgcc agaagagcgt
     5101 ccggcagctg gtgcggttat caacgaagcg aaagagcagg ttcagcaggc gctgaatgcg
     5161 cgtaaagcgg aactggaaag cgctgcactg aatgcgcgtc tggcggcgga aacgattgat
     5221 gtctccctgc cgggtcgtcg cattgaaaac ggcgggctgc atccggtgac ccgtaccatc
     5281 gaccgtatcg aaagtttctt cggtgagctt ggctttaccg tggcaaccgg gccggaaatc
     5341 gaagacgatt atcataactt cgatgctctg aacattcctg gtcaccaccc ggcgcgcgct
     5401 gaccacgaca ctttctggtt tgacgctacc cgcctgctgc gtacccagac ctctggcgta
     5461 cagatccgca ccatgaaagc tcagcagcca ccgattcgta tcatcgcgcc tggccgcgtt
     5521 tatcgtaacg actacgacca gactcacacg ccgatgttcc atcagatgga aggtctgatt
     5581 gttgatacca acatcagctt taccaacctg aaaggcacgc tgcacgactt cctgcgtaac
     5641 ttctttgagg aagatttgca gattcgcttc cgtccttcct acttcccgtt taccgaacct
     5701 tctgcagaag tggacgtcat gggtaaaaac ggtaaatggc tggaagtact gggctgcggg
     5761 atggtgcatc cgaacgtgct gcgtaacgtt ggcatcgacc cggaagttta ctctggtttc
     5821 gccttcggga tggggatgga gcgtctgacc atgttgcgtt acggcgtcac cgacctgcgt
     5881 tcattcttcg aaaacgatct gcgtttcctc aaacagttta aataaggcag gaatagatta
     5941 tgaaattcag tgaactgtgg ttacgcgaat gggtgaaccc ggcgattgat agcgatgcgc
     6001 tggcaaatca aatcactatg gcgggcctgg aagttgacgg tgtagaaccg gttgccggta
     6061 gcttccacgg cgtggtcgtt ggtgaagtga ttgagtgtgc gcagcatccg aacgctgaca
     6121 aactgcgtgt gacaaaagtg aatgtcggcg gcgatcgcct gctggacatc gtctgcggtg
     6181 cgccaaactg ccgtcagggc ctgcgtgtgg cggtagcgac cattggtgct gttctgccgg
     6241 gcgatttcaa aattaaagcg gcgaaactgc gtggcgaacc gtctgaaggg atgctgtgct
     6301 ccttttctga gctgggaatt tctgacgatc acagcggcat tatcgagctg cctgcagatg
     6361 cgccgattgg caccgacatc cgcgaatacc tgaaactcga tgacaacacc atcgaaatca
     6421 gcgtaacgcc aaaccgtgcc gattgcttag gcattatcgg cgtggcgcgt gatgttgccg
     6481 tgctgaacca gctgccgctg gttgaaccgg aaatcgttcc ggttggtgcg gccatcgacg
     6541 acacgctgcc gattacagtc gaagcgccgg aagcctgccc gcgctatctt ggccgtgtgg
     6601 taaaaggcat taacgttaaa gcgccaactc cgctgtggat gaaagaaaaa ctgcgtcgtt
     6661 gcgggatccg ttctatcgat gcagttgttg acgtcaccaa ctatgtgctg ctcgaactgg
     6721 gccagccgat gcacgctttc gataaagatc gcattgaagg cggcattgtg gtgcggatgg
     6781 cgaaagaggg cgaaactctg gtgctgcttg atggcactga agcgaagctg aatgctgaca
     6841 ccctggtcat cgccgaccac aacaaggcgc tggcgatggg cggcatcttt ggtggcgaac
     6901 actctggcgt gaatgacgaa acgcaaaacg tcctgctgga atgcgctttc ttcagcccgc
     6961 tgtctatcac cggtcgtgct cgtcgtcatg gcctgcatac tgatgcgtct caccgttatg
     7021 agcgtggcgt tgatccggca ctgcagcaca aagcgatgga acgtgcgacc cgtctgctga
     7081 tcgacatctg cggtggtgag gctggtccgg taattgatat caccaacgaa gcaacgctgc
     7141 cgaagcgtgc caccattact ttacgtcgca gcaaactgga tcgcctgatc ggccatcata
     7201 ttgctgatga gcaggtaact gacattctgc gtcgtctcgg ctgcgaagtg accgaaggca
     7261 aagacgaatg gcaggccgtt gcgccgagct ggcgtttcga catggagatt gaagaagatc
     7321 tggtcgaaga agtcgcgcgt gtttacggct acaacaacat cccggatgag ccggtacagg
     7381 caagcctgat tatgggtact caccgcgaag ccgatctgtc gctcaagcgc gtgaaaacgc
     7441 tgctcaacga caaaggctat caggaagtga tcacctacag cttcgttgat ccgaaagtgc
     7501 agcagatgat ccatccaggc gttgaagcct tactgctgcc aagccctatt tccgttgaaa
     7561 tgtcagcaat gcgtctttcc ctgtggaccg gcctgctggc aaccgtggtg tacaaccaga
     7621 accgtcagca gaaccgtgtg cgcattttcg aaagtggtct gcgtttcgta ccagatactc
     7681 aggcaccgtt gggcattcgt caggatctga tgttagccgg tgtgatttgc ggtaaccgtt
     7741 acgaagagca ctggaacctg gcaaaagaga ccgttgattt ctatgatttg aaaggcgatc
     7801 ttgaatccgt tctcgacctg accggtaaac tgaatgaggt tgagttccgt gcagaagcga
     7861 atccggcact gcatccgggg caatccgcag cgatttatct gaaaggtgaa cgtattggtt
     7921 ttgttggggt tgttcatcct gaactggaac gtaaactgga tcttaacggt cgcactctgg
     7981 tgttcgaact ggagtggaac aagctcgcag accgcgtggt gcctcaggcg cgcgagattt
     8041 ctcgcttccc ggcgaaccgt cgtgacatcg cggtggtggt cgcagaaaac gttcccgcag
     8101 cggatatttt atccgaatgt aagaaagttg gcgtaaatca ggtagttggc gtaaacttat
     8161 ttgacgtgta ccgcggtaag ggtgttgcgg aggggtataa gagcctcgcc ataagcctga
     8221 tcctgcaaga taccagccgt acactcgaag aagaggagat tgccgctacc gtcgccaaat
     8281 gtgtagaggc attaaaagag cgattccagg catcattgag ggattgaacc tatggcgctt
     8341 acaaaagctg aaatgtcaga atatctgttt gataagcttg ggcttagcaa gcgggatgcc
     8401 aaagaactgg ttgaactgtt tttcgaagag atccgtcgcg ctctggaaaa cggcgaacag
     8461 gtgaaactct ctggttttgg taacttcgat ctgcgtgata agaatcaacg cccgggacgt
     8521 aacccgaaaa cgggcgagga tattcccatt acagcacggc gcgtggtgac cttcagaccc
     8581 gggcagaagt taaaaagccg ggtcgaaaac gcttcgccca aagacgagta atctgatcta
     8641 actaaaaagg ccgctctgcg gccttttttc ttttcactgt cgaagagtca ccgtaaaatc
     8701 aacgccatga cacttcagca gaacggatac catgctgaca cttgcccgcc aacaacagcg
     8761 acaaaatatt cgctggttat tatgcctgtc agttttgatg ctgctggcgc ttctcttaag
     8821 cctttgcgcc ggtgaactat ggatctcgcc aggtgactgg ttttctcctc gtggcgaact
     8881 gttcgtctgg caaattcgcc tgccacgtac gctggctgta ttgctggttg gtgcggcgct
     8941 ggctatatcc ggcgctgtaa tgcaggcgtt gtttgaaaat cctctggcag aacctggact
     9001 acttggcgtc tctaacggcg caggcgtggg gcttatcgcc gcggtattgc ttgggcaagg
     9061 gcaactcccc aactgggcgc tagggctgtg tgcgattgct ggcgcgctta tcatcacttt
     9121 aatactctta cgtttcgctc gtcgtcatct ttcgaccagt cggttattgc tggctggcgt
     9181 tgcattaggg attatctgta gcgcactaat gacgtgggct atctactttt ccacctctgt
     9241 tgatttacgt cagctgatgt actggatgat gggcggtttt ggcggcgtag actggcggca
     9301 aagctggctg atgctggcat tgatccccgt gttgttgtgg atctgttgtc agtccaggcc
     9361 gatgaatatg ttagcacttg gcgagatctc ggcgcggcaa ctgggtttac ccctgtggtt
     9421 ctggcgcaat gtgttggtcg cagcgaccgg ctggatggtt ggcgtcagtg tggcgctggc
     9481 gggtgctatc ggctttattg gtctggtgat cccacatatt ctccggctgt gtggtttaac
     9541 cgatcatcgc gtattacttc ccggctgcgc gctggcaggg gcgagcgcat tgctgctggc
     9601 cgatattgta gcgcgcctgg cattagctgc cgcagagctg cctattggcg tggtcaccgc
     9661 aacgttgggt gcgccggtgt ttatctggtt attgttaaaa gcaggacgtt agccgcaaaa
     9721 agacggtcta tgattaaaag ctaagatttt tactgaccac acccaggaga aacgatgcaa
     9781 gattccattc tgacgaccgt agtgaaagat atcgacggtg aagtgaccac gctggagaag
     9841 tacgccggta atgtgctgtt gattgtcaat gtcgcctcaa agtgtggctt aacgccgcaa
     9901 tatgagcagt tggagaatat tcagaaagcc tgggccgatc gtggttttgt ggtgctggga
     9961 ttcccgtgca accagtttct ggaacaagaa ccgggcagcg atgaagagat taaaacttac
    10021 tgtaccacca catggggggt gacgttcccg atgttcagta agattgaagt taatggcgaa
    10081 ggacgccatc cgctgtatca aaaattgatt gccgcagcgc cgaccgcagt cgcgccggaa
    10141 gagagcggat tctatgctcg tatggtcagc aaaggtcgtg caccgctgta ccctgatgat
    10201 attttatgga attttgagaa atttttagtt ggcagggacg gattggtcat ccagcgtttt
    10261 tccccggata tgacgccgga agatcccatt gtgatggaaa gcattaaact ggcgttggca
    10321 aaataatgtc tattgtgatg cagttacagg atgttgcgga atctacccgc ctggggccgc
    10381 tttctggcga ggttcgggct ggggagatcc tgcacctggt ggggccgaat ggcgcgggta
    10441 agagtacctt actggcgcga atggccggaa tgaccagcgg taagggaagc attcagttcg
    10501 cggggcaacc actggaagca tggtccgcaa caaaactcgc gctacatcgc gcctatcttt
    10561 cacaacacca gacgccaccg tttgcaatgc cggtctggca ctacctgaca ctgcatcagc
    10621 acgataaaac gcgtaccgaa ctactgaatg atgtcgcagg ggcgctggct cttgatgaca
    10681 aactcggacg gagcaccaat caactttccg gcggtgaatg gcaacgcgta cgtcttgctg
    10741 cggtggtgtt gcaaatcaca ccacaagcca atcccgcagg ccaattgctg tttcttgatg
    10801 agccgatgaa cagtcttgat gttgcgcaac aaagtgcgtt agacaaaatt ctgagcgcac
    10861 tgtgtcagca aggactggcg attgtgatga gcagtcacga tctcaaccac accttgcgtc
    10921 atgcgcatcg ggcgtggttg ctaaaaggtg gaaaaatgct ggccagtgga cgcagggaag
    10981 aggtgctcac gccgccaaat ctggcgcagg cctatgggat gaattttcgc cgtctggata
    11041 tcgaaggtca cagaatgctg atttcgacca tctgatttca ttaatatctt gaaaatcggg
    11101 taattacgac gctaaattaa tgcaagaata aaaaaacaga ggaaccgtac ggaatgcgtt
    11161 tctgccttat tttgatcaca gcactgtttc tggccgggtg tagccaccat aaagcaccgc
    11221 cgccaaatgc cagactttct gattcgatta ccgttattgc cggtttgaac gaccagctac
    11281 aaagctggca tggcacgccg tatcgttatg gtggcatgac gcggcgcggt gtggactgtt
    11341 cggggtttgt ggttgtgacg atgcgcgatc gtttcgattt gcagctgccc cgtgaaacca
    11401 aacaacaagc ctctatcggc acgcaaattg ataaagacga gttgctgcct ggtgacctgg
    11461 tctttttcaa aacgggctcc ggacaaaatg gtttgcatgt aggtatttat gataccaaca
    11521 accaatttat ccacgcctct accagcaagg gagtgatgcg ttcctcactt gataatgtct
    11581 attggcagaa aaatttctgg caggcgagac gaatctagta tgtcgaaaaa tggacggcga
    11641 tgatacatcg ccgtccattt actcctgaat acagtagtat tttgcagaca aaaattccgt
    11701 aaatggatta ttcgtttata atcaggataa tctaaaaaaa ggacccctga aaaagtggtt
    11761 aacatggaac aaatgaaagt tactccttct gtaattgtta tgtcttatgg cctcatccct
    11821 tttagccgga tactgaaaaa catccttcga gagggacggt taccatgaag atttttttgg
    11881 agaatcttta tcattctgat tgctatttcc tgccgataag agataaccag caagaccttg
    11941 tcggcgttga gttaattacc catttctcca gtgaagatgg tacggttcgt atcccgacga
    12001 gtcgggttat agcgcaactt accgaggagc aacattggca attattttca gagcagttgg
    12061 aattactaaa atcatgccaa cactttttta ttcaacataa actctttgcc tggcttaatt
    12121 taacgccaca agtggctaca ttattattag acagggataa ttacgcaggc gaactattaa
    12181 aatatccgtt tattgaactg ttgattaatg agaattaccc gcatctcgat gaaggaaaag
    12241 ataatcgcga tcttttgtcc ttatcgcaaa tgtatcccct tgtgttaggt aatttgggcg
    12301 caggcaatag cacaatgaaa gcggtttttg atggtctttt cacccgcgtc atgctggata
    12361 aaagttttat tcagcaacaa atcacgcatc gctccttcga accgtttatt cgtgcgatcc
    12421 aggctcaaat ttccccatgt tgcaattgca tcatcgcggg gggcattgat acgccagaaa
    12481 taatggcaca gattaccccc tttgatttcc atgctctcca gggatgcctg tggcctgccg
    12541 ttcctgtaaa tcagataacg acgttggttc agcgataacc ctcctgtttg ctggtgttta
    12601 agacgagagt aaccgtctac actatcaaat aggaggatct atgaccctgt cttttgttac
    12661 ccgctggcgc gatgaattgc cagaaaccta tacagcactt tcccctacgc ctttaaataa
    12721 tgcccggctg atttggcata ataccgaact cgctaacacg ctgagtattc catcgtcgct
    12781 gtttaaaaat ggcgcaggtg tctggggcgg cgaaacccta ctgcctggta tgtcaccact
    12841 ggcgcaggtt tacagtggtc atcagttcgg cgtctgggcg ggccaactgg gtgatgggcg
    12901 cggcatttta ctcggcgaac aacggcttgc tgatggcact acaatggact ggcatctgaa
    12961 aggtgctggc ctgacgcctt attcgcgaat gggtgatgga cgggcggttt tacgttcgac
    13021 gatacgagaa agtctcgcca gtgaggcgat gcattatctg ggcattccga cgacccgcgc
    13081 gttaagtatc gtcaccagcg attcgccagt gtatcgggaa acggtggagc caggcgcgat
    13141 gctgatgcgt gtggcactaa gtcatctgcg ctttggtcat ttcgaacatt tttactatcg
    13201 ccgcgagccg gaaaaggttc gtcagttggc tgactttgcc attcgtcatt actggtctca
    13261 tcttgaagat gatgaggaca aataccgtct ctggtttagc gatgttgtcg cacgtaccgc
    13321 atcgttaatt gcccaatggc agacggtcgg ctttgctcat ggggtgatga ataccgacaa
    13381 catgtcgctg ctggggctga cgcttgatta cgggccgttt ggttttcttg atgattacga
    13441 acccggtttt atttgtaatc actcggatca ccaagggcgt tacagctttg ataatcaacc
    13501 tgccgtcgcg ttgtggaatt tacagcgtct ggcgcagaca ttgtcgccat ttgtcgccgt
    13561 tgatgccctg aatgaggccc tggacagcta tcagcaggtt ttgttgacgc attatggaca
    13621 acggatgcgg cagaaactgg gcttcatgac ggagcaaaaa gaggataacg cgctactaaa
    13681 tgaattattc agtctgatgg cgcgagagcg cagcgattat acccgcacat ttcgcatgct
    13741 gagtctgacc gagcagcaca gcgcggcgtc accgctacgt gatgagttta ttgatcgtgc
    13801 ggcatttgat gactggtttg cccgttatcg gaggcgtttg caacaagacg aggttagcga
    13861 tattgagcgt cagcaactga tgcaaagcgt taacccagct ctggtgttgc gcaactggtt
    13921 ggcgcaacgg gcgattgaag cggcagaaaa gggtgatatg acggaattgc accgcctgca
    13981 tgaggcgttg cgaaatcctt tcagcgacag agatgatgac tacgccagtc gtccacctga
    14041 ctggggtaaa cggctggaag tcagctgctc gagttaaagg ttacttggtc aacagcagct
    14101 tgccagcctg agttttacgg agcagatatt cttgcccgtc atgatcgata atcagtttgc
    14161 catccggacc taacagtgtc tggctggtaa ttcgccggat gggctgcggg gaagcggtct
    14221 tgtgattggc atccgtttca ggcgtgagtt ttctgctatc cgtataacgc ataaaaatta
    14281 ataataattt atataacaat gattcttatt atcaatatta tggcgggcca gagcaagcag
    14341 taatttgttg agaagacaac aagttgcgca gaaatgccca tcccgtcagg aatgggcacg
    14401 cattcagaag cgagtatcta ccgcagaggc gagtttttcg accaggcgtt cggtatcttc
    14461 ccagcccaga cacgggtcgg taatggattg accgtaagtg agcggctgac cgccgacgat
    14521 tttttgcgtt ccttcgcgca ggaaactttc cgccataatt ccagcaatcg ccgtagatcc
    14581 attgcggatt tgctgacaaa tatcctcaca aacttctaac tggcgtcggt gctgcttctg
    14641 gcagttaccg tggctgaaat ccaccaccag atgttcaggt aaatcaaact cgtgcagcgt
    14701 atcgcaggct gcggcgatat catcggcatg ataattcggt tttttgccgc cacgcataat
    14761 aatgtggcca tacgggttgc cgctggtctg atagatggtc atctgaccat ttttgtctgg
    14821 cgagaggaac atatggctgg cgcgggctgc gcggatagca tccacggcaa tccgcgtgtt
    14881 gccatcggta ccatttttaa aacctaccgg acaggagagt gccgaagcca tttcacggtg
    14941 gatctgactt tcggtagtac gtgcgccaat cgcgccccaa ctgattaaat cagcaataaa
    15001 ctgaccggtc accatatcga ggaactcagt cgcggtaggg acgcccagct catttacctg
    15061 taaaagtaat ttgcgcgcca gctccagacc gtgatttacc cgatagctgc cgtttaaatc
    15121 tggatcggag attagccctt tccagccgac aacagttcgt ggtttttcaa aataggtgcg
    15181 cattacgatt tccagccgtg actggtactg gttgcgcagc gactgcagac gggtggcgta
    15241 ctccattgca gcggtgagat cgtggatcga gcaggggcca ataatgacca acagtcgctt
    15301 atcttcacca ttcagtattt tttcaattct gcggcgggag tcggtgacat gggtggcgac
    15361 gccaggcgtt acgggatacc gtagcgcgag ttcggcgggc gttaccaggc tctcaatacg
    15421 cgcagtacgg agttcgtcag ttctgttcat tacaagtctc agaaatttgt tgcttctacg
    15481 gcaggactac cgggaagtga tgcgcatcac gataaaccaa tcgcagctga tttcaagtgc
    15541 gaggtgtgtc acacctcgcc gggtggttag catgataaca aaaaaacaag ctaatgcact
    15601 agttctctag tacattcgac ggctaaggcc catgatatcg aggatcttgg tggcaatctc
    15661 ttctaccgaa taattggtac tgttaatcca cgggatctga tttttacggt acaacgcttc
    15721 cacttccgcg acttccatcc tgcactgacg aagcgaggca tagcgactgt tctcccgacg
    15781 ttcctcgcga atcgccgcca gacgttccgg gtcgatagtc aggccgaaca atttatgctg
    15841 aagcggtttg agcgacgcgg gtagcacaag attatccata tcgtcggcaa taaaggggta
    15901 gtttgccgcg cggataccaa actgcattgc cagatacaga ctggtggggg ttttaccaca
    15961 gcgagaaacg ccgagcagga tcacctgagc ctggtccaga ttgcgtaacg aaatgccatc
    16021 atcgtgggcg agggtgtaat caatcgcagc aatgcgcgca tcatatttat tgagattatt
    16081 agggttaagg ccatgggtac gatgagcaat cggcgttgga tccagtttca tctcttgttg
    16141 tagcggggca accagcgcct gaacgatatc ctggcaaaag ccctcacttt gcaagatgat
    16201 ggcgcgaatc tccggcaaca cgatggagta gaagaccagc gggcgcacac ctgtctggtg
    16261 ataaattgcg tcaatctgat ccttcactgc ccgtgcacgg ctctcatttt cgacaaacgg
    16321 cagcgtaatg ctgctgatag tgacgggaaa ttgtgacatt actgcgtgtc ctaatacctc
    16381 cgcagttatt gccgtaccat cagaaatata aaaaacgtgg cgatcaacag cattatccat
    16441 tttgttcttc ccgtgatgca gacataattc tatgaaagca taaat
//
LOCUS       DAZUCC010000110        16146 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZUCC010000110 DAZUCC010000000
VERSION     DAZUCC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.110"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            328..834
                     /locus_tag="ACHY09_002970"
     CDS             328..834
                     /locus_tag="ACHY09_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS3885918.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRTPDDEGDLEERLLRARAELTEEQAVAQKLKNQVTEGKLIDTGFCIF
                     ALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLD
                     EYIRATTE"
     gene            806..2734
                     /locus_tag="ACHY09_002971"
     CDS             806..2734
                     /locus_tag="ACHY09_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFS3885919.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVVPMTAVEWADQNYYLPKES
                     SYGDGEWKTLPFQVAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVAGYFIEHKSRNCL
                     LFQPTDSAAEDFMKSHVESTIRDVPCLKALSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVHYTAAVPDR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGAEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2718..2924
                     /locus_tag="ACHY09_002972"
     CDS             2718..2924
                     /locus_tag="ACHY09_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFS3885920.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRKIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2921..4513
                     /locus_tag="ACHY09_002973"
     CDS             2921..4513
                     /locus_tag="ACHY09_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS3885921.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYHGGGAGFGGQMAKWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFPGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVRAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLIHILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGQRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4503..6008
                     /locus_tag="ACHY09_002974"
     CDS             4503..6008
                     /locus_tag="ACHY09_002974"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFS3885922.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEFMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVRQDMQQRIDAAHRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPD
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTAMFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            6045..6392
                     /locus_tag="ACHY09_002975"
     CDS             6045..6392
                     /locus_tag="ACHY09_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS3885923.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            6450..7478
                     /locus_tag="ACHY09_002976"
     CDS             6450..7478
                     /locus_tag="ACHY09_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS3885924.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPTYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NIIQGSGKEWSKQDRDTFDPTYDIDLFCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAADGLRCYGAIQDAQALSEGVVASSRYPKHWQTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7530..7898
                     /gene="gpFI"
                     /locus_tag="ACHY09_002977"
     CDS             7530..7898
                     /gene="gpFI"
                     /locus_tag="ACHY09_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS3885925.1"
                     /translation="MATKEENLNRLRELAGLLGREADMSGSAADIAQRVAEWEEEVCA
                     PENEIADVDDAVHEQAYRNTGEDAFGILERIRLLKCFYLCGVDDETGEPVEHVDAGRV
                     ILMPPSVAKDMVRSGMAVYA"
     gene            7891..8244
                     /locus_tag="ACHY09_002978"
     CDS             7891..8244
                     /locus_tag="ACHY09_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS3885926.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGIRAQFISGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            8259..8837
                     /locus_tag="ACHY09_002979"
     CDS             8259..8837
                     /locus_tag="ACHY09_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3885927.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPGASAMAINRVATTAINQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKKNIERIRRERLPEELAYALKQQLRIAIKR"
     gene            8834..9229
                     /locus_tag="ACHY09_002980"
     CDS             8834..9229
                     /locus_tag="ACHY09_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFS3885928.1"
                     /translation="MKHTDIRAAVLDALEQHKHGATLFDGRPVVFEEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            9237..9986
                     /locus_tag="ACHY09_002981"
     CDS             9237..9986
                     /locus_tag="ACHY09_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3885929.1"
                     /translation="MATPNPLAPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPQTGNVAKGQSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            10002..10424
                     /locus_tag="ACHY09_002982"
     CDS             10002..10424
                     /locus_tag="ACHY09_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFS3885930.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQCFDYMRFVSDAGQQETTEHD
                     AVRINQRYLDTASLLVAMSLWHTHPLKGTLVSPETEMQQIRREVMLGWPADALNQATN
                     RVLSLSGMLDNRSDADPEQTGKAGTTESVTSKKRSKAS"
     gene            10451..10864
                     /locus_tag="ACHY09_002983"
     CDS             10451..10864
                     /locus_tag="ACHY09_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFS3885931.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCAHYFQDTQLDAHFSGLMY
                     AVLSLFFCDPEMHPADFSLLVPKTEEELTAMPDENDVLIQKAEGLAGGVRFGGGEGRD
                     ILPSADVEDVPVDDAALMMASAGIYGGVRYVPASR"
     gene            10845..13460
                     /locus_tag="ACHY09_002984"
     CDS             10845..13460
                     /locus_tag="ACHY09_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS3885932.1"
                     /translation="MSRPAGDLVIDLSLDAARFDEQMARVRRHFSGMEADARKTAAAV
                     EQSLGRQARAAQKAGISLGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDATDSAEDSLSDTANALAENARNAGELGRFMSVARLAA
                     GGGVAALAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAGVISDEAGGGVR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTADAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KDVPGVSALFGELAEENREFIEKTKRDELALRKSIAERDARIRQGEMGYIRRSRATGV
                     SKGPGQQEAVSRLAEELKGKNRTSRQTRSAGEREEEQAREALLVLEAELRMLEKHSGA
                     NEKISQQRRALWKAESQYAVLKEAATKRQLSGQEKSLLAHEKETLEYRRQLAELGDKV
                     EYQKRLNELAQQAARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPQA
                     LAQVTGALKQTWADEDMLRGDWLAGLKSGWGEWSERATDSFSQVKSAATQTFDGIAQN
                     MAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGGAIGGAFAGGGAHVSTGT
                     AISSAAANFNFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAE
                     GGYVGGEGSPAQMRRAEGIRFEQNNNVVIQNDGTGGQPGPQLLKAVYDMARKGARDEI
                     QAQMRDGGVFSGGGR"
     gene            13457..13786
                     /locus_tag="ACHY09_002985"
     CDS             13457..13786
                     /locus_tag="ACHY09_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3885933.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRTAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            13786..14484
                     /locus_tag="ACHY09_002986"
     CDS             13786..14484
                     /locus_tag="ACHY09_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS3885934.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGEECGFTGGAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            14495..15238
                     /locus_tag="ACHY09_002987"
     CDS             14495..15238
                     /locus_tag="ACHY09_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS3885935.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVTGTPEGERYQPCVNVSAEPE
                     AYFRIAPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFHRKDDWWRNGQNLYLDNLAEN
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            15235..15816
                     /locus_tag="ACHY09_002988"
     CDS             15235..15816
                     /locus_tag="ACHY09_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS3885936.1"
                     /translation="MNTAAEAIRALSLQVPGFRSQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSFFTGGATMALWGAALSAGGL
                     TATTVLFSLGASMILGGVAQMLAPKAKTPDYRATDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT         3678 a         3838 c         5182 g         3448 t
ORIGIN      
        1 gtcgcctgtc cggtgcgatg gtggaagaaa ctggattttg ttgcaactga taaccattat
       61 cattttcacg ggtcctttcc ggcatatgga cccgttacgg ggcggcgacc tcgcgcgttt
      121 tcactattta tgagattttt tgagggggtg gttgttgttt aattgtttgg tatatctaat
      181 tgataagtaa ggtgaaaata aaataaatac aacaacctta cgatgtgttt tgatgtcatt
      241 aatgtgaaaa aattcaatga tatcaaatgt ttttgtaaaa acacatggtt gttgtatcgc
      301 tttttatcga tgacttatgg agaggagatg gcctttttat tgaataaaag tgatatggcc
      361 tcctccatcg gtatctctgt tcaggcattt gataaatggg gcgttcctcc tgttgagcgt
      421 cgggggaggg aagtttttta tgacgttaaa actgtactgg agatagatcg cgagcggcga
      481 caacacaatc agagaacacc tgatgacgag ggcgatctgg aggaaaggct gcttcgggcc
      541 agagctgaac tgacagaaga acaggccgta gctcaaaaac ttaaaaatca ggtaaccgaa
      601 ggtaagctta ttgacaccgg attctgtatt tttgccctca gtaagctggc aatggcgtta
      661 tccagtacgc ttgattccat ccctttatcc atgcagcgac agtttcctga tttaacaccg
      721 cgccatcttg accatctgaa aacccttatt gcgaagggag caaatcagtg tgcgcgggca
      781 ggggataaat taccggattt gctggatgaa tatatcagag caacaactga ataatatgat
      841 ggctgccgtt tcggttgcgc tgcagcctct ggtcagggtt gtgccgatga cggcagttga
      901 atgggctgat caaaattatt atctgccgaa agaatcctca tacggtgacg gcgaatggaa
      961 aacgctgccg ttccaggtcg ccatcatgaa ctgtatgggt aacgaccagg ttcgcacggt
     1021 taatctgata aaatctgccc gtgttggcta tacaaagatg ttgctgggtg tcgccgggta
     1081 ttttattgaa cataaatccc gaaactgtct gctttttcag cccacggatt ctgccgctga
     1141 agattttatg aaatctcacg tggaatccac gattcgcgat gttccctgtc tgaaagccct
     1201 ttctccctgg ctgggacgta aacatcgtga taataccctc acgctgaaac gcttttcatc
     1261 gggtgtgggt ttctggtgcc tgggtggtgc tgccgccaaa aactaccgtg aaaaatccgt
     1321 ggacgtggtc tgctatgacg aactttcctc gttcgagccg gatgtcgaaa aagagggctc
     1381 gccaaccctg ctgggggata aacgtattga gggttctgta tggccaaaat ccattcgcgg
     1441 ctcgacgcca aaaataaaag gcacctgcca gatcgaaaaa gcggccaacg agtcggcgca
     1501 tttcatgcgt ttttatgttc cctgcccgca ctgtggggag gagcagtatc tgaaatttgg
     1561 cgatgaatcc acgccttttg gccttaaatg ggagaaggac agccccgaaa gcgttttcta
     1621 cctctgtgaa catcatggct gcgtgatcca tcagtctgag cttgaccaga gcaacgggcg
     1681 ctggatctgt gaaaacacgg ggatgtggac ccgtgacggc ctgatgtttt tcagcgcccg
     1741 gggtgatgaa attccaccac cgcgctccat cacgttccat atctggacgg cgtacagtcc
     1801 gttcaccacc tgggtacaga tagtctatga ctggctggat gcactgaaag atcccaacgg
     1861 cgtgaaaacc tttgtgaaca ccacgctggg cgagacctgg gaagaggctg tgggcgaaaa
     1921 acttgatcac caggtgctga tggataaggt cgtgcattac acggcggcgg tacctgaccg
     1981 ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga tgtatgtctg
     2041 gggatgggca ccgggagagg aagcctttct ggtggataaa atcatcatta tgggccgtcc
     2101 tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aacaaaaaat accgccatgc
     2161 agatggtgcc gaaatgacca tttcccgtgt ctgctgggac accgggggga tcgatggcga
     2221 aatcgtttat cagaggtcaa aaaaacacgg tgttttccgg gtgctgccgg taaaaggcgc
     2281 atctgtctat ggcaagccgg tgatcaccat gccaaaaacc cgcaatcagc ggggcgtgta
     2341 tctgtgtgaa gtggggacgg acaccgcaaa agaaattctc tatgcccgta tgaaagccga
     2401 tcccacgcct gcggatgaag ccacgtcgta tgccatccgt tttcctgatg atccggagat
     2461 tttttcgcag acagaggcgc agcaactggt ggcggaagag cttgtggaga agtgggaaaa
     2521 aggaaagatg cgtctgctgt gggataacaa aaagcggcgt aacgaagcgc tggactgcct
     2581 ggtgtatgcc tacgcggcat tacgtgtgtc cgtgcaacgc tggcagcttg atctggctgt
     2641 actggcaaaa tcccgggaag aagagacgac ccggccaacc cttaaagaac tggcagcgaa
     2701 gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt caggcgcgcc
     2761 ttgatttatt aaccggtaaa cgggtggtgt ctgtccagaa agatggtcgc aaaattgaat
     2821 atacggcggc ctctctggat gagcttaacc gtgcgatcaa tgatgctgag tcggtactgg
     2881 ggacaacccg acgtcgccgt cgtccgctgg gagtgaggtt atgaaacgaa cgcctgtcct
     2941 gattgatgtg aacggcgttc cgcttcgtga gagtctcagc taccacgggg gcggcgcagg
     3001 atttggcggg caaatggcga agtggttgcc accggcgcag agtgccgatg cggccctgct
     3061 gcccgcgttg cgtctgggga atgcccgggc agatgatctg gtgcgcaata acggaatagc
     3121 ggccaatgcg gttgccctgc ataaggatca cattgtcggg catatgtttc tgatcagcta
     3181 ccgtccgaac tggcgctggc tggggatgcg ggagactgcg gcaaaaagtt ttgtcgatga
     3241 ggtggaggcg gcctggtcgg aatacgccga agggatgtct ggcgagatcg acgtggaagg
     3301 gaaacgcacg tttacggaat ttatccgtga aggtgtgggc gttcatgcct ttaacggcga
     3361 aatctttgtg cagccggtct gggatacgga gagcacgcag ctgtttcgta cgcgttttaa
     3421 agccgtgagt ccgaaacggg tggacacgcc gggacacggt atggggaacc gctttctgcg
     3481 ggccggtgtg gaagtcgatc gatatggccg tgccgttgcg taccatatct gtgaggatga
     3541 ttttccgttc ccgggtagtg gtcgatggga gcggatcccg cgtgaacttc ccaccgggcg
     3601 tccggccatg ctgcatattt tcgagccggt ggaggacggg cagacccgtg gggccaatca
     3661 gttttacagc gtcatggaac ggctgaagat gctcgattcc ctgcaggcaa cacagcttca
     3721 gtcggccatt gtcagggcaa tgtatgcagc gacgattgaa agtgaacttg ataccgaaaa
     3781 ggcctttgaa tatatcgccg gtgcgccgca ggggcagaag gataatccgc ttattcatat
     3841 tctggagaag ttctccagct ggtatgacac gaatcacgtg acgctgggcg gtgtcaaaat
     3901 tccgcacctt tttcccggtg atgatctgaa actgcagact gcgcaggatt cagacaatgg
     3961 attttctgcg cttgaacagg cgctgctgcg gtatatcgcc gccggtcttg gcgtttccta
     4021 cgaacagttg tcccgtgatt actcgaaggt cagttactca agtgcccgcg cctccgccaa
     4081 tgagtcgtgg cgctatttta tgggacaacg aaaatttata gcgtcccggc tggccacgca
     4141 gatgttttcc tgctggctgg aagaggcact tcttcggggg attattcgtc cgccacgggc
     4201 gcgttttgat ttttatcagg cgcgttcagc ctggtcacgg gcagagtgga ttggtgccgg
     4261 aagaatggcc attgacgggc tcaaggaagt ccaggaatca gtgatgcgca ttgaggccgg
     4321 actgagcacg tatgagaaag agctggcgct gatgggcgag gattatcagg acattttccg
     4381 ccagcaggtc agggaatccg cagagcggga aaaagccgga ctctcacgcc cggtgtggat
     4441 agcgcaggcg tatcagcagc agatagcgga gagtcgcagg ccggaagagg agacaacacc
     4501 acgtgagacg taatctttca cacataattg ccgcagcatt caatgaaccg ctgcttctgg
     4561 agcccgccta tgcgcgggtt ttcttttgcg cgctggggcg cgagatgggg gcagcaagtc
     4621 tttcggtacc acaacagcag gtacagcttg atgctcccgg aatgctggct gaaacggatg
     4681 agttcatggc cggaggtaaa cgaccggccc gtgtttaccg ggtggtgaac ggtattgcgg
     4741 tactgccggt gaccggcacg ctggtgcacc ggctgggtgg tatgcggcca ttttccggaa
     4801 tgacaggtta tgacggcatt gtagcctgtc ttcagcaggc aatggcagac acctctgtcc
     4861 gtggcgtact gctggacatt gacagtccgg gcggtcaggc cgccggtgcg tttgactgcg
     4921 ctgacatgat ttaccgcctc cgccagcaga agcctgtctg ggcactgtgc aatgacacgg
     4981 cctgttctgc ggccatgctg ctggcgtcgg cctgctcccg acggctggtt acccagacat
     5041 cccgtatcgg ctccattggc gtgatgatga gccatgtcag ctatgccggt catctggcgc
     5101 aggccggagt ggatatcacg ctgatttatg ccggggcgca caaggtggat ggcaatcagt
     5161 ttgaagcctt accggcagag gtgcgtcagg acatgcagca gcgcattgat gcggcgcacc
     5221 ggatgtttgc cgaaaaagtg gcgatgtata ccgggctgtc tgtggatgcg gtcacgggga
     5281 cagaggctgc cgtttttgaa ggtcagtccg gtattgaggc cgggctggcg gatgaattaa
     5341 tcaatgcgtc ggatgccatc agtgtgatgg ctgcggcgct gaacagtaat gtcagaggag
     5401 gcactatgcc gcaattaacc gcaacggaag ctgccgcgca ggagaaccag cgggtgatgg
     5461 ggatcctgac gtgtcaggaa gcgaaaggac gtgaacagct tgccacgatg ctggcaggac
     5521 aacagggcat gagcgttgaa caggcccggg cgattctggc cgcggcagca ccacagcagc
     5581 cggtggcatc cgcgcagagt gaagccgatc gcattatggc gtgtgaagaa gctaaaggtc
     5641 gtgaacaact ggcggcaacg ctggcggcga tgccggatat gacggtggaa aaagcccgcc
     5701 cgatcctggc tgcttcaccg caggcggatg ccggcccctc actccgtgat cagattatgg
     5761 ctctggatga ggcaaaaggg gctgaggcgc aggctgaaaa actggcggcg tttcccggaa
     5821 tgacggtgga ggctgcccgc gacattctgt cctcatctcc ggataaagca gaaccggttt
     5881 ctgcatccac aaccgccatg tttgaacgca tcatggcgaa ccattcaccg gcagcggtac
     5941 aggggggcgt gtcacagacc tcggcagacg gtgatgcgga cgtgaaaatg ctcatggcca
     6001 tgccatgaag tcagtgctga ccatcaatat gaggttttta caatatggta acgaaaacca
     6061 tcactgaaca acgtgcagaa gtacgtattt ttgccggtaa tgatccggct cataccgcca
     6121 caggcagcag cgggatttct caggcaacac cggcactgac gcccctgatg ctggatgaag
     6181 ccagcgggaa actggtggtc tgggacggac agaaagccgg tagtgcggtt ggcatactgg
     6241 tactgccgct tgaaggcaca gagacggtgc tgacgtatta caagtcgggg acctttgcga
     6301 cggaggcaat ccgctggcct gaaagtgtgg atgaacacaa aaaggccaac gcctttgcag
     6361 gaagtgccct gagtcacgcg gcgctgccgt aacacgttat caggccacca cggtggcctg
     6421 actgatttct gaatgaaagg aactgattta tgggattgtt tacgacccgc cagttactcg
     6481 gttataccga acaaaaagtg aaatttcgtg cgctgtttct ggagctgttt ttccgccgta
     6541 cggtgaactt ccacaccgaa gaggtgatgc tggacaaaat taccggaaaa acgccggtgg
     6601 cagcctatgt ttctccggtc gttgaaggaa aagtactgcg tcatcgtggt ggtgaaaccc
     6661 gcgtgttgcg tccgggctac gtcaagccga aacacgaatt taattaccag caggcggtgg
     6721 agcgccttcc cggtgaagat ccggctcaac tgaacgaccc gacctaccgt cgtctgcgta
     6781 tcatcaccga taacctcaaa caggaagagc atgccattgt tcaggtggaa gaaatgcagg
     6841 cggtgaatgc cgtgctgtat ggcaaataca ccatggaagg agaccagttc gagaaaattg
     6901 aggtcgattt tggcagatcg acgaagaata acatcataca gggtagcggt aaggagtggt
     6961 caaaacagga ccgtgacacg ttcgacccga catatgatat cgaccttttc tgtgatcagg
     7021 ccagcggtct tgtgaatatt gccattatgg acggtaccgt ctggcgtctg ctgaatggct
     7081 ttaagctgtt ccgcgaaaaa ctggataccc gtcgcggctc aaattcacaa ctcgaaacgg
     7141 cagtgaaaga tctgggcgca gtggtgtcct tcaaggggta ttacggcgat ctggccattg
     7201 tggtggcgaa aacgtcttat gtggcagagg acggtaccga aaaacgttat ctgccggagg
     7261 gcacgctggt cctggggaat acggctgcag atgggctccg ttgttacggt gccattcagg
     7321 atgcgcaggc gttgtccgaa ggtgtggtgg cttcttcccg ttacccgaaa cactggcaga
     7381 ccgtgggcga tccggcccgt gaattcacca tgacgcagtc cgcaccgctg atggtgctgc
     7441 cggatccgga tgagtttgtg gtggtgcagg tgaaataatc cgtgagcggg ggcgaaatgc
     7501 ccccgtgtct tttttcacag gaggctgaga tggcaacaaa agaagaaaat ctgaatcgtc
     7561 ttcgtgaact ggctggcctg ctggggcgcg aggcggatat gtcggggagt gctgcggata
     7621 tcgcacagcg tgtggccgag tgggaggagg aggtttgtgc cccggaaaat gaaatcgcag
     7681 atgttgatga tgccgttcat gagcaggcat acaggaacac cggtgaggat gccttcggta
     7741 ttctggaacg tatcaggctt ctgaagtgtt tttacctgtg cggtgttgac gatgaaacag
     7801 gggagcctgt tgagcatgtt gatgctggca gggtaattct gatgcctccc tcagtggcaa
     7861 aagacatggt caggagcgga atggccgttt atgcgtgatt ttcagaatgc cttcgatgct
     7921 gcccttgccg gggtggacag cacgattgtt gaagtgatgg gcatcagggc gcagttcatc
     7981 tccggtgcgc agcgtggcgg cgaagttcag ggggtttttg acgatccgga gtcgctgggt
     8041 tttgccggtg gcggggtccg tattgaagga agcagcccgt cattatttgt acggacagat
     8101 acggtgcgtg ctgtacggcg tggtgacact ctgaccatta acggcgagat gttctgggtg
     8161 gatcgtgttt ctccggatga cgggggcagc tgttatctct ggctcaaccg ggggcagcca
     8221 cccgcagtta accggcgacg ataaagacag gtgaaattat ggcgataaaa gggcttgatc
     8281 aggcaattga caatctgagc cgggtccgta aaaacgccat tcctggtgct tcagcaatgg
     8341 ccattaaccg cgtggccaca acggcaataa atcagtcggc atcacaggtt gcccgtgaaa
     8401 ccagggtgcg ccggaaactg gtaaaggaaa gggccaggct taaaagggcc acggtcaaaa
     8461 atccgcaggc cagaatcagg gttaaccggg gggatttgcc cgtaatcagg ctgggtaatg
     8521 cgcgggttgt cctgtcccga cgcaggcgtc gtaaaaaggg gcagcgttca tccctgaaag
     8581 gtggcggcag cgtgcttgtg gtgggtaacc gtcgtattcc cggcgcgttt attcagcaat
     8641 taaaaaatgg ccggtggcat gtcatgcagc gtgtggccgg gaaaaaccgt taccccattg
     8701 atgtggtgaa aatcccgatg gcggtgccac tgaccacggc gtttaaaaag aatattgaac
     8761 ggataaggcg tgaacgtctg ccggaagaac tggcatacgc gctgaaacaa caactcagaa
     8821 ttgcgataaa acgatgaaac atactgatat ccgtgcggca gtgctggatg cactggagca
     8881 gcataaacac ggggcgacgc tgtttgatgg tcgccccgtt gtttttgagg aagaggattt
     8941 tcctgcgatc gcggtttatc tgacggatgc agagtatacc ggtgaagagc tggatgcaga
     9001 tacctggcgg gccacactgc atattgaggt gtttttaccg gcacaggtac cggattcaga
     9061 acttgatcag tggatggaaa gccggattta tccggcgatg acagcgatcc ctgcactggc
     9121 aggcctgatt accacgatgg ttacgcaggg ctatgagtat cgtcgtgatg acgatatggc
     9181 gttatggagc tcggcagatc tgacttattc cattacctac gaaatgtgag gacgatatgg
     9241 caacaccaaa tcccctggcg ccggtaaaag gttccggcac cacgctctgg gtttacaccg
     9301 gtaagggcga tgcttatgca aatccgctgt ccgatgacga gtggacgcgc ctggcaaaaa
     9361 taaaggatct gacgccgggc gagatgacgg cggaatccta cgacgataac tatctggatg
     9421 atgaggatgc ggactgggta tccaccgggc agggccagaa atctgccggt gacaccagtt
     9481 ttacgctggc ctggaagccc ggcgagaaag ggcagcgtga tttgattgcc tggtttgaca
     9541 gcagtgagac ccgggcttac aaaatccgct tcccgaacgg cacggtggat gtgttccgtg
     9601 gctgggtgag tgccattggt aaagccgtga cggctaaaga ggtgatcacc cgcaccgtga
     9661 aaatcaccaa catcggtcgt ccgtcgctgg cggaagatcg gggtgaaatc acgccggtca
     9721 ccggtattac cgtgacaccc cagacgggga atgtggcaaa aggtcagagc accaccctga
     9781 ccgtggccgt tcagccggaa ggggcgacgg ataaaacgtt ccgcgccacg tcggcgaatc
     9841 agaatttcgc caccattacc gtgaaaggga acacgatcac cgtgaaaggt gttgcggcag
     9901 gtaaagcgca gatccctgtt gtcaccggta acggtgagtt tgcggcggtg gcggaaatta
     9961 ccgtcacgga tggcgctgcg ggctgaggga ggagataaag tatgttcctg aaaacagaac
    10021 aatttgaata taacggtgtg tccgtcacgc tttccgaact gtctgcgctg cagtgctttg
    10081 attatatgag gtttgtttca gacgcaggac aacaggagac aacggagcat gatgccgtgc
    10141 gtattaacca gcgctatctg gatacggcgt ccctgcttgt ggcgatgtcg ctctggcaca
    10201 cgcatcctct caaaggcaca ctggtctctc cggagacaga gatgcagcag atccgccgtg
    10261 aagtgatgct gggatggcct gctgatgcac tgaatcaggc aacgaaccgg gtgctttcgc
    10321 tttcaggtat gctggataac cggagtgatg cagatcctga acaaaccggg aaagcaggca
    10381 caactgagtc ggtaacatca aaaaagcgtt cgaaggcgag ctgaattttg tcctgaaact
    10441 ggcgcgcgag atggggaggc ctgactggcg cgccatgctt gccgggatga catccacgga
    10501 atatgccgac tggcgacgtt tttactgcgc gcattatttt caggacacac aactggatgc
    10561 acatttttcc gggctgatgt acgccgtact cagcctgttt ttttgcgatc cggagatgca
    10621 tccggcggat ttcagtctgc ttgtaccgaa gacagaggaa gaactgacgg caatgccgga
    10681 cgaaaatgat gtactgatac agaaggcgga gggccttgcc ggtggtgtcc ggttcggtgg
    10741 gggcgaaggg cgcgatattt taccttctgc ggatgtggag gatgtcccgg tggatgatgc
    10801 cgcattaatg atggcttcag cggggattta cggaggtgtg agatatgtcc cggccagcag
    10861 gtgatctggt tattgatttg agtctggatg cggcccggtt cgatgaacag atggcccggg
    10921 tcaggcgtca tttttccggg atggaggcgg atgcaagaaa aaccgccgct gctgttgaac
    10981 agagcctggg ccgtcaggcc cgggctgcac aaaaagccgg aatatccctc gggcagtata
    11041 aggcggccat gcgcacactg cccgcacagt tcacggatat agtcactcag cttgccggtg
    11101 gtcagaatcc cttccttatc atgcttcagc agggtgggca gatcagcgac tccttcggcg
    11161 ggccgctcag cctgcttacc ctgctgaagg aggaacttct cgggatcagg gatgccactg
    11221 actcagcaga ggattcgctg tcagatacgg caaatgcact ggctgaaaat gcccggaatg
    11281 ccggtgaact ggggcgattt atgtcggtgg cccgtctggc ggcaggcggc ggggttgccg
    11341 cactggccgc gcttgctgct gccgcctggc aggcagagca ggctgatcgg gccttattgc
    11401 gttcactgac cctgaccggc ggggctgcgg ccaccacaac ggcagaattg tggaaaatgg
    11461 ccggggtgat cagcgatgaa gccggtggtg gcgtcagaca ggcggcagaa aatctggccc
    11521 gtctggcaga aagcggaaaa tataccgccg ggcagctccg gatcatgggg gaaacctcgc
    11581 agcggtggct gcagacggtg ggggacgatg ccgggaaggt ggaaaaagcc tttgaaggga
    11641 ttgcagcaga tccggtgaag gcgctggcct ccctgaatca gcagtataac ttcctgagcg
    11701 tctcccagtt gcgccatatt gatgagcttg agcgcacgaa aggtaaacag gctgcggtga
    11761 cggaggcgat gtccctgttt gcggatgtca tgaatgcgcg tctggagcaa ctcgataaag
    11821 cggccacgcc ggtggaaaaa atctgggacg atgttaaaac ctggaccgct gacgcatggg
    11881 catggatagg tgatcacacg ctgggggcac tcagtctgat cactgacgtg gtggccggaa
    11941 ccgttgagca ggtgaagctg ctgcttgtgc agggggatct ggcgctggct gaatttattc
    12001 agtcagcctg ggaaacgaca aaagatgtcc ccggcgtcag tgcgctgttt ggcgagctgg
    12061 cagaagagaa ccgggaattt attgagaaaa caaaacgcga tgagctggcg ctgagaaaat
    12121 ccattgcgga acgggatgcg cgtatacgcc agggggaaat ggggtatatc aggcgttcgc
    12181 gtgcaacagg cgtcagcaag ggaccggggc agcaggaagc cgtcagccgt ctggctgaag
    12241 agctgaaagg taaaaatcgc acatcccggc aaacgcgctc tgccggggag agggaggaag
    12301 agcaggcaag ggaagcgctg cttgtccttg aagctgaact ccggatgctg gaaaaacaca
    12361 gcggtgcgaa tgagaaaatc agccagcagc gccgggcgct gtggaaggcg gaaagccagt
    12421 atgcggtcct gaaggaggcg gccacaaaac ggcagttatc cgggcaggaa aaatccctgc
    12481 tggcccatga gaaagagacg ctggagtaca gacgccagct ggctgagctg ggcgacaagg
    12541 ttgaatacca gaaacggctc aatgagctgg cacagcaggc ggcgcgtttt gaacagcagc
    12601 agagcgcgaa gcaggcagcc atcagcgcaa aagcccgcgg tctcactgac cgtcaggcgc
    12661 agcgggagtc tgaagagcag cgtcttcgtg acgtgtacgg cgataatccg caggcgctgg
    12721 cacaggtcac cggggcactg aaacagacat gggcggatga agacatgctg cgcggtgact
    12781 ggctggccgg gctgaagtcg ggctggggcg agtggtctga gagagcgacg gacagttttt
    12841 cgcaggtgaa aagtgcggcc acgcagacct ttgatggtat tgcacagaat atggcggcga
    12901 tgctgaccgg tgcagaggca gactggcggg gattcacccg ttccgtgctg tccatgatga
    12961 cagaaattct gctgaagcag gccatggtgg ggattgtcgg gcgtatcggc ggtgccattg
    13021 gcggtgcctt cgccgggggc ggggcgcatg tctccacggg aacggccatc agcagtgcgg
    13081 cggcgaactt caactttgcg accggcggat ttacgggcac gggtggcaaa tacgaacctg
    13141 cggggattgt tcaccgcggt gagtttgtct tcacgaagga ggcgaccagc cgtattggcg
    13201 tcggtaacct gtaccgcctg atgcgggggt atgcggaagg cggttatgtg ggaggtgaag
    13261 gaagtccggc gcagatgcgt cgcgctgagg gtatccggtt tgagcagaac aacaacgtgg
    13321 tgattcagaa tgacggtacc ggcggtcagc cagggccgca gttgctgaaa gcggtgtatg
    13381 acatggcccg taagggggcg cgggatgaga ttcaggcgca gatgcgtgac ggtggcgtat
    13441 tttccggagg cgggcgatga aaacatttcg ctggaaagtg aagccggata tggaggtgaa
    13501 ctcgcagccg tcggtgcgtg aagtgcgttt tggtgacggg tattcgcagc ggacggcggc
    13561 aggactgaat gctgacctga aaacctacag ggtgacgctt tccgtgaccc gggaggaggc
    13621 ccggcatctg gaagcgttcc tggcagagca cgggggctgg aaggcatttc tgtggacgcc
    13681 gccctatgcc tggcggcaga taaaggtgac ctgtgccggg tggtctgcgc gggtcgggat
    13741 gctgcgcgtt gaattcagcg cggagtttaa gcaggtggtg aactgatgca ggatattcac
    13801 gaagaaagcc tgaacgagtc ggttaagtca gagcagtcac cgcgggtggt gctctgggaa
    13861 atcgacctga cggtgcaggg cggtgagcgg tattttttct gtaatgagct caatgaaaaa
    13921 ggggagccgg tgacctggca ggggcgtgaa tatcaggcgt acccgattga ggggagtggc
    13981 tttgagatga acgggaaggg cagcagtgcc cgcccgtcgc tgacggtgtc caatctgttc
    14041 ggtctggtca ccgggatggc tgaagacctg cagagcctgg tgggtgccac ggtggtccgc
    14101 cgccgggtgt atgcgcgttt tctggatgcg gtgaattttg tggcaggcaa tccggaggcg
    14161 gacccggagc aggagctgac ggaccgctgg gtggtggagc agatgtcaga gctcacagcc
    14221 atgacggctt cgtttgtgct ggcgacaccg acggagacgg acggtgcgct gtttcccggt
    14281 cgcatcatgc tggcgaacac ctgtatgtgg gattaccggg gagaggagtg cggtttcacc
    14341 ggcggggcag tggcagatga gttcgacaac cccaccacgg atatccggaa ggacagatgc
    14401 agtaaatgca tgcgcgggtg tgagatgcgc ggcatggtgg ctaattttgg cggtttcctt
    14461 tccattaata aactttcgca gtaaatcccg ttttatgaca cagactgaat cagcgattct
    14521 ggcgcatgcc cggcggtgtg cgccagcgga gtcgtgcggc ttcgtgacag gcaccccgga
    14581 gggcgaacgg taccagccct gtgtgaatgt ctccgcagag ccggaggcgt attttcgtat
    14641 tgcaccggaa gactggctgc aggcacagat gcagggggag attgtggcgc tggtccacag
    14701 tcatcccggt ggtctgccct ggctgagcga ggcggaccgg cggctgcaga taaagagtgc
    14761 cctgccctgg tggctggtct gccggggtga cattcacaaa ttccgctgtg tgccgcacct
    14821 gacgggacgg cgctttgagc acggggtgac ggactgttac accctgttcc gggatgcata
    14881 ccatctggcg gggataacgc tgccggattt tcaccgcaag gatgactggt ggcgcaacgg
    14941 ccagaacctg tacctggaca acctggcgga aaacggcttt taccgggtgc ccctgtcctc
    15001 tgcacaggcg ggcgatatcc tgctgtgctg ctttggcgca tcggtgccga atcatgccgc
    15061 gatttactgt ggcaacggtg agctgcttca ccatataccg gaacaactga gtaaacggga
    15121 gaggtattct gaaaaatggc aacgacgaac gcattctgtc tggcgtcacc gccactggtc
    15181 cgcatctgcc ttcacgggga tttacaacga tttggtcgcc gcatcagcct gtatgtgaac
    15241 acggcagcgg aggccatccg tgccctgtcg ctgcaggtgc cgggattccg cagtcagatg
    15301 aacgaaggct ggtaccagat acgcattgcc ggtgaggata ccgcgccgga ggcggtgtat
    15361 gcccgtcttc atgagccgct gagcgggagg gccgtgattc atattgtacc gaggctggca
    15421 ggggccgggg gaaatggtgt gtttcaggtg gtgctggggg ttgcagcgat tgtcgggtca
    15481 tttttcactg gtggtgcaac gatggcgttg tggggcgcag ccctgagtgc cggagggctg
    15541 actgccacca cggtgctgtt ctcgctgggt gccagcatga tactgggtgg tgtggcccag
    15601 atgctggccc cgaaggcaaa gacgccggat taccgggcga cggataacgg caaacagaac
    15661 acgtattttt cgtcactgga caacatgatt gcccagggca acccgatgcc ggtgccttac
    15721 ggtgaaatgc tggttggttc acgacggata tcccaggata tcagcacccg tgatgagggc
    15781 ggtgacggta aggtggtggt tatcgggcgg ggataaaaat aaaaaaatcc cgcagtgttc
    15841 ggaggctgcg ggagataaca atgaagacta acgttcaagg agttttgttt ttattgaccc
    15901 gaaaaaactg taatgccccg gaatgatatc tgccacgggg atgtacagaa aatgtgaaga
    15961 atttcagaaa ttttctgttg cggggctgaa gtaaaaaacg ctcccgcaat acacctggat
    16021 attaacggga ggaacgaata gtcaccttaa ggagttgcga ttaattgctt ttattgaccg
    16081 caggtaatct acccatccgg caaggtttcc tccacgggaa tttgccggaa atgtgaagaa
    16141 tttcag
//
LOCUS       DAZUCC010000111        16134 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZUCC010000111 DAZUCC010000000
VERSION     DAZUCC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.111"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            135..671
                     /gene="hpt"
                     /locus_tag="ACHY09_002989"
     CDS             135..671
                     /gene="hpt"
                     /locus_tag="ACHY09_002989"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS3885937.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            complement(712..1374)
                     /gene="can"
                     /locus_tag="ACHY09_002990"
     CDS             complement(712..1374)
                     /gene="can"
                     /locus_tag="ACHY09_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFS3885938.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            1483..2409
                     /locus_tag="ACHY09_002991"
     CDS             1483..2409
                     /locus_tag="ACHY09_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3885939.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            2406..3176
                     /locus_tag="ACHY09_002992"
     CDS             2406..3176
                     /locus_tag="ACHY09_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3885940.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            3281..3721
                     /locus_tag="ACHY09_002993"
     CDS             3281..3721
                     /locus_tag="ACHY09_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS3885941.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLV
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            3785..5014
                     /locus_tag="ACHY09_002994"
     CDS             3785..5014
                     /locus_tag="ACHY09_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS3885942.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYTTLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5018..5398)
                     /gene="panD"
                     /locus_tag="ACHY09_002995"
     CDS             complement(5018..5398)
                     /gene="panD"
                     /locus_tag="ACHY09_002995"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFS3885943.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            5672..6628
                     /locus_tag="ACHY09_002996"
     CDS             5672..6628
                     /locus_tag="ACHY09_002996"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFS3885944.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVRQEVRQEVRQEV
                     IQEVRQEVRQEYALRFLSKGMSREDVAEMANLPLAEVDKLIN"
     gene            6697..6912
                     /locus_tag="ACHY09_002997"
     CDS             6697..6912
                     /locus_tag="ACHY09_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3885945.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGFQKGYKEGLQKVRLAQRLL
                     SKGMSREDVAEVTALSLAEIDKLINSN"
     gene            complement(6994..7845)
                     /gene="panC"
                     /locus_tag="ACHY09_002998"
     CDS             complement(6994..7845)
                     /gene="panC"
                     /locus_tag="ACHY09_002998"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFS3885946.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(7857..8651)
                     /gene="panB"
                     /locus_tag="ACHY09_002999"
     CDS             complement(7857..8651)
                     /gene="panB"
                     /locus_tag="ACHY09_002999"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFS3885947.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(8763..10052)
                     /locus_tag="ACHY09_003000"
     CDS             complement(8763..10052)
                     /locus_tag="ACHY09_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFS3885948.1"
                     /translation="MQHDNGVKMKIFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDY
                     GVGPGDQKWRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGG
                     YLYCNRSNTASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTN
                     ISSSGMYIGDSPTQYFRWIPESEATLYWSCNNANSSNKYWAVGGVWQSLTVEFYTDTN
                     FDPTKTNKVTLSKPGGYLYSFKGYGLGGGINDHSYFLKIDFDLDNMTLSNPTCFTAAL
                     TGPSVSGSTVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKEL
                     LANTLTGNNAAKGVGVLIEGLKNTKSAQMVLKPNDSTSVYKDYETEDDTTGGIYPDKG
                     NGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            complement(10078..10674)
                     /locus_tag="ACHY09_003001"
     CDS             complement(10078..10674)
                     /locus_tag="ACHY09_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFS3885949.1"
                     /translation="MRPTQRNLMKKSILLLSLLFSTTFPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNTGAKNVGVVIFSIQDPANTFNVLGVTGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGQLISHVLVDIYYE"
     gene            complement(10701..11309)
                     /gene="yadL"
                     /locus_tag="ACHY09_003002"
     CDS             complement(10701..11309)
                     /gene="yadL"
                     /locus_tag="ACHY09_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFS3885950.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDIEMG
                     TCTSTLTDDGTAKLSTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTPR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAG
                     ATGGDVVDYPMSAVLVVAKDKTVTDVTAGDFTAPATFTVTYN"
     gene            complement(11321..11893)
                     /locus_tag="ACHY09_003003"
     CDS             complement(11321..11893)
                     /locus_tag="ACHY09_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3885951.1"
                     /translation="MIKTTPHKIAILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPKMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFIYQ"
     gene            complement(11910..14510)
                     /locus_tag="ACHY09_003004"
     CDS             complement(11910..14510)
                     /locus_tag="ACHY09_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFS3885952.1"
                     /translation="MTIKSTKKYHNHLTRIATFCALLYSNSALCAELVEYDHTFLMGK
                     DASNIDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFTVIEGKKNAQACITQKNLL
                     QFHISSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASG
                     NYNWMTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPVLASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVT
                     IEEADGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNN
                     YLTGYTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWN
                     KLFENTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLN
                     EDGQTDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTN
                     RISYSVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADI
                     YDEQNNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSI
                     ILNGLRCQIQ"
     gene            complement(14545..15285)
                     /locus_tag="ACHY09_003005"
     CDS             complement(14545..15285)
                     /locus_tag="ACHY09_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFS3885953.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGIPSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDVKMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            complement(15394..15984)
                     /locus_tag="ACHY09_003006"
     CDS             complement(15394..15984)
                     /locus_tag="ACHY09_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3885954.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFSNSKGTLNNDMSINNPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDATS
                     KTATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
BASE COUNT         4070 a         3646 c         3937 g         4481 t
ORIGIN      
        1 atcacaattc gagctttatt aacagattcc gcgaatgaat agttttactg gtatactgcg
       61 tgtcttgcgc tttgttgcgg tgctgaaacc tgcccgtgcg aagtgatttg tttttaaatc
      121 atatggttag agatatgaaa catactgtag aagtaatgat ccccgaagcg gagattaaag
      181 cgcgtatcgc cgaactgggt cgtcagatta ctgagcgtta caaagacagt ggcagcgata
      241 tggtgctggt tggtctgctg cgtggctcat ttatgtttat ggcggacctg tgccgtgaag
      301 ttcaggtatc tcatgaagtc gattttatga ccgcctccag ctacggtagc ggcatgtcca
      361 ccacccgtga tgtgaaaatc ctcaaagatc tggatgaaga tatccgtggc aaggacgtgc
      421 tgattgttga agatatcatc gactcgggga atacactgtc gaaagtgcgt gagatcttaa
      481 gcctgcgcga accgaagtcg ttggcgattt gtacgctgct ggataaaccg tcccgtcgtg
      541 aagtgaacgt cccggtagaa tttatcggtt tctcgatccc ggatgagttt gtggtgggtt
      601 acggcattga ttacgcacag cgttaccgtc atctgccgta tatcggcaaa gtgattctgc
      661 tggacgagta agtgtgaagt tgccggatgt gttgcatccg gcatggcatt tttatttgtg
      721 gttggcgtgt ttcagcttga ggttggagat cccgtgacgg taacgttgct caagggtttc
      781 gcggttagtc gctgtgacat ccagatcacg cagcaagccg tcgtgaatgc cgtaggccca
      841 gccgtgaatg gtaactttct gcccgcgttt ccacgctgat tgcataatgg tggagtggcc
      901 caggttatac acctgttcca tgacgttcag ttcacacaag gtatccagac ggcgctcttg
      961 cggcatttcg ccgagcaatg agctatgttt gaaccagata tcgcggatat gcagcagcca
     1021 gttgttgata agccccagtt ccgggttttc aactgcggct tgtacgccgc cgcaaccgta
     1081 gtggccacag ataataatgt gttcaacttc gagtacatcc actgcatact gaaccacgga
     1141 aaggcagttc aggtcggtgt gaatgaccag gttagcaaca ttacggtgaa caaagagttc
     1201 gcccggctca agaccggtta aacgttctgc aggaacgcga ctgtcggaac atccaatcca
     1261 tagaaagcgc ggtttttgcg cttgtgccag tttctcaaaa aacccgggat cctcttccac
     1321 cagcattttt gaccatagtg cattgttgct gatgagtgta tctatgtctt tcatggaggt
     1381 taacgacctg taaccaaata attacgtttg gctaatatag ggcaactccg ggacgattta
     1441 aaccacagat aaagtgtaag aacgtaaggt aagtaaaaat ttatgaccat tgcactggaa
     1501 cttcaacagc ttaaaaaaac ctatccaggc ggcgttcagg cgcttcgtgg aatcgatttg
     1561 caggtcgaag cgggtgattt ttatgcgctt ctcgggccga acggggccgg aaaatcgacc
     1621 actatcggta ttatcagctc tctggtaaat aaaacctccg ggcgggtcag cgtatttggt
     1681 tacgatctcg agaaagatgt cgtaaacgct aaacgtcagt tgggtctggt gccacaggaa
     1741 tttaacttca acccgtttga aaccgtgcag caaattgtgg tgaatcaggc agggtactac
     1801 ggtgtggagc gcaaagaagc gtacattcgc agcgaaaagt atcttaaaca actcgatcta
     1861 tggggaaaac gcaacgaacg tgcgcgtatg ttatctggcg ggatgaagcg ccgtttaatg
     1921 attgcccgtg cgttaatgca tgaacctaaa ctactgattc tcgacgaacc gaccgcaggc
     1981 gtggatatcg aacttcgccg ctcaatgtgg ggctttttga aggatttaaa cgacaaaggc
     2041 accactatca ttctcaccac acactacctg gaagaagcag aaatgctgtg ccgcaatatc
     2101 ggcattattc aacacggtga gctggtggaa aatacctcga tgaaggcgct gctggcgaag
     2161 ctgaaatcgg aaacctttat tctcgatctc gcaccgaaaa gcccgttacc gaagctcgat
     2221 ggctatcagt atcgactggt cgataccgcg acgctggaag ttgaagttct gcgtgagcag
     2281 gggatcaaca gcgtatttac gcagttaagt gagcagggca ttcaggtatt aagtatgcgt
     2341 aacaaagcta accgtctgga agagctgttt gtttcactgg ttaatgaaaa acaaggagat
     2401 cgcgcatgat gcatctttac tgggtggcac taaaaagcat ctgggcgaaa gagatccatc
     2461 gctttatgcg tatctgggtg cagacgctgg tgccaccggt catcaccatg accctttact
     2521 ttattatctt cggtaacctg attggttcgc gtattggcga tatgcatggc ttcagctaca
     2581 tgcagttcat tgtaccgggg ctgatcatga tgtcggtcat caccaacgcc tacgctaacg
     2641 tcgcgtcatc attttttggt gccaagtttc agcgtaatat tgaagagctg ctggtagcgc
     2701 cggttccgac tcacgtcatt attgccggat atgtcggcgg tggcgtggcg cgtggtctgt
     2761 ttgttggcat tctggtgacg gcaatttcac tgttttttgt gccgtttcag gtgcattcgt
     2821 gggtatttgt cgctttaacg ctggtgctca cggcggtgtt gttctccctt gcgggtttgc
     2881 tgaacggcgt gtttgccaaa acgttcgatg acatcagcct ggtgccaacc tttgtgttaa
     2941 cgccacttac gtatttgggc ggggtctttt actcactgac tttgttgccg ccgttctggc
     3001 aagggctgtc gcacctgaac ccaatcgttt atatgatcag tggtttccgc tacggcttcc
     3061 tcggtatcaa tgatgttccg ctggtcacta ccttcggtgt actggtggtc tttattgtgg
     3121 cgttttattt gatctgttgg tcgctgatcc aacgtggacg tggtttgcgt agctaaggct
     3181 atttcctctc ctctggattt gggggagagg agttttgacg gctatcaccc tttatcaaca
     3241 atggtcaggg tagactgatt ttcggctaag gaggaaggcg atgttaggtt gggtaattac
     3301 ctgtcacgat gaccgggcgc aagagatact ggatgcgctg gagaaaaaac atggggcact
     3361 tcttcagtgc cgggccgtga atttctggcg tgggttaagc tctaatatgc tcagccgcat
     3421 gatgtgcgat gctctgcatg aagcggactc tggtgaaggt gtcatcttct taaccgatat
     3481 agccggagcg ccaccgtacc gcgtggcttc gctattaagc cataaacatt cccgttgcga
     3541 agtgatttct ggcgtcacgt taccgttaat tgagcagatg ctggtctacc gtgaaacgtt
     3601 atccagcgca gagtttcgcg agcgtattgt cgaactgggt gcgccggagg tgagtagtct
     3661 ttggcaccag caacaaaaaa atccgccttt cgtcctcaaa cataatttgt atgagtatta
     3721 acccgcgatt ctgatgacgc ttttgctaca ataaaagcgt tgtttcaccc tcggttattt
     3781 tttcatgtac aaacaagctg ttattctcct gctgatgctg tttaccgcaa gcgtcagtgc
     3841 cgcgttacct gcccgttata tgcaaaccat cgaaaatgct gcggtctggg cgcaaattgg
     3901 cgacaagatg gtgaccgtgg ggaatattcg ggccgggcaa atcattgccg tggagcccac
     3961 tgccgcaagt tattacgcat ttaattttgg ctttggtaaa gggtttatcg ataaaggtca
     4021 tctcgagccg gttcaggggc gacaaaaagt tgaagacggt ttgggtgacc tcaacaagcc
     4081 gctgagtaat cagaacttag ttacctggaa agatacgccg gtctataacg cgccgagtgc
     4141 gggcagtgcg ccgtttgggg tactggcgga caatttgcgc tacccgattt tgcataaact
     4201 gaaagacagg ttaaatcaaa cctggtatca gatccgtatt ggcgatcgac tggcctatat
     4261 cagcgcgctg gatgcccaac ccgataatgg cctgccggtg ctaacctatc accatattct
     4321 gcgcgacgaa gaaaacaccc gttttcgcca tacttcgacg accacctcgg tacgcgcttt
     4381 caataaccag atggcctggc tgcgcgacag gggatacacg acactgagta tggtgcagct
     4441 ggaaggttac gtgaagaata agatcaatct ccctgcgcga gcggtggtga ttacctttga
     4501 tgatggcctc aaatcggtga gccgctatgc gtatcctgtg ttgaaacaat atggcatgaa
     4561 ggcgacggcg tttattgtta cctcgcgcat caaacgtcac ccgcagaagt gggacccaaa
     4621 atcgctgcaa tttatgagcg tttctgaact taacgaaatt cgcgatgtat ttgatttcca
     4681 gtcacatacc cattttttgc atcgggtgga tggttatcgc cgacccatat tgctgagccg
     4741 tagtgagcac aatattctgt ttgattttgc acgttcacgc cgcgctctgg cgcaatttaa
     4801 tccgcatgtc tggtatcttt cgtatccgtt tggcggattt aatgacaaag ccgtgaaggc
     4861 agcaaaagaa gccggatttc acctggcggt gacaaccatg aaaggcaaag taaaaccggg
     4921 ggataatccg ttgttactaa aacgacttta tatcttaaga acggattcgc tcgagacgat
     4981 gtcgcggctg gtgagtaacc agccgcaggg gtgattatca ggcaacctgt accggaatcg
     5041 ctttcgcggt acgtttcatt tcattgtcgc cttcaaaata ggcgacgttg ggtcgccagg
     5101 tgcgagcttc ttcatctggc atagtaacga agctggcgat gatgacaata tcgccgacgc
     5161 tggcgcagtg ggccgccgca ccgttaacgg aaataattct cgaaccgcgt tctgccgcga
     5221 tggcataggt ggagaaacgc ttgccgttgg tcacattcca gatatcaatg gcttcgtttt
     5281 cgagaatacc agctgcgtca agaaaatcct ggtcaatggc gcaagaacct tcatagtgca
     5341 ggtccgcatg agtcactttc acgcggtgga gtttgccctg cagcatcgtg cgaatcataa
     5401 cttctacctt tctaccctgt cgttaacgaa gcaggcgatg cctgctttga ggaaattctc
     5461 acgcagtatt gcccgatttt tgtttagtgt ctactcatct gacggcattt gcatcagcag
     5521 tttgcgtacc gtgccgaagc gtgccatgta acgcctgtaa ccaattgaaa tttctctgaa
     5581 cgctgctcgg taaacaataa agaggcggct gacgacggcg cgccttgact ggatactgcc
     5641 ttcacgcaag acaacacgga aggcagggaa gatggatgca ccgagtacca caccgcatga
     5701 cgcggtattt aaacaatttt taatgcatgc ggaaacggct cgtgactttc tggatatcca
     5761 tttgccagcg gaactacgcg aactgtgtga cctcgacacg ctgcatcttg agtcggggag
     5821 ttttattgaa gaaagcctga aagggcacag cactgacgtg ctttattccg tgcaaatgca
     5881 gggtaatccc ggttatttgc atgtggtgat tgaacatcaa agcaagccgg ataagaaaat
     5941 gacctttcgc atgatgcgct atgccattgc cgccatgcac cggcatctgg aggcggatca
     6001 cgataagctg ccgctggtgg tgccgattct gttttatcag ggcgaggcca caccttatcc
     6061 gctctcaatg tgctggtttg atatgtttta ctcgccggag ctggcgcgac gcgtttacaa
     6121 caaccctttc ccgctggtgg atatcaccat cacaccggat gacgaaatca tgcaacatcg
     6181 gcggattgcg attctcgaac tactgcaaaa acatattcgc cagcgcgact taatgttatt
     6241 gctggagcaa ctggtcacgc tgattgacga agggtacact agcggaagtc agttagttgc
     6301 catgcaaaac tatatgctgc aacgcggtca tactgaacaa gcggatttgt tttatggtgt
     6361 gctgagagac agggaaacgg gaggggagtc tatgatgacg ctggcgcagt ggtttgaaga
     6421 gaagggaaga caggaggaaa ggcaggaggt aaggcaggag gtaagacagg aggtaagaca
     6481 ggaggtaaga caggaggtaa tacaagaggt tagacaggaa gtaagacagg aatacgccct
     6541 gcgttttctg agtaaaggga tgtctcggga agacgttgca gagatggcaa atttacctct
     6601 tgctgaggtt gataagctga ttaactaagt tatctgcatt atcagttata gccagataat
     6661 tgttattgtt aactgaccag ataattaagg gaacatatgg aaataaagac acggagagaa
     6721 accaggcaga cgttggcgca gtggtttgaa gagaagggat ttcagaaggg atttcagaag
     6781 ggatataaag agggactcca gaaggtaaga ctggcccagc gtcttctgag taaaggaatg
     6841 tctcgggaag acgttgcaga ggtgacagct ttatctcttg cagagattga taagctaatt
     6901 aactctaact aagttgtcta cgatatgaat aattgcctac agataacgtg gcaatgaata
     6961 actggcgcca tcagccgtag cgccagataa ggattacgcc agttcgacga ttttgttgtc
     7021 gatcaggcgg gcatcgccaa gccaggcggc taccagaatc actgcacgtt tgctgttttc
     7081 agagacctcc agcaaggtgt cggcatcacg aatctgaata tcatcggcgc ggaagccttt
     7141 ttcattcagt tcttgcccgg caatagtaat aatttcatcg agatcccgtt cgccagcctg
     7201 caatttgtcg gcaatcgaac ttaaaacttt gtacagaccg ggcgcaattt tgcgttgttc
     7261 cgccgtcaga taaccgttac gggaactcag cgccagaccg tctttggcgc gcataattgg
     7321 cacaccgaca atctcaatat caaagcccat atcagcaacc attttgcgga tcagcgccag
     7381 ttgctgaaaa tctttttcac cgaagcaggc gatgtccggc tggaccaggt tgaacagctt
     7441 gctgacaatc gtcgaaacgc cgcgaaaatg tcccggacgg ctggcacctt ccagcatggt
     7501 ggaaagacca ggaacatcaa cataggtgtg ggtttctgta ccgttcgggt agatctcttt
     7561 taccgaaggg gcgaaaacta aatccacttt acgtttgttt agcttttcgc agtcctcttg
     7621 caaggtccgt ggataacgtg ccagatcttc cgggcggtca aactgcatcg ggttaacgaa
     7681 aatactgacc actaccacat cggcgcgggc tttggcttcg tcgaccagct tcatatggcc
     7741 atcgtgcagg ttacccatgg tgggcaccag cgccacgcgc ttaccttcca tacgcaggcg
     7801 gcgaatttgc tgacgcagca gcggcagggt ttcgataatt aacacaacgt cactccttaa
     7861 tggaaactgt gttcttcgcc cgggtaaacg ccggactcca cttcagccat atactgccgc
     7921 acagccgcgc ggatgtcgcc cgtttcggcg aggaaatttt tagcgaattt aggaatgtgg
     7981 ccgccggtaa tgccgaacgc gtcgtgcatc acgaggatct gcccgtcagt gacgttgcct
     8041 gcgccaatgc caataaccgg gatcgccagt gcttcggtaa tacgttttgc cagttcaacc
     8101 ggcacgcatt ccagcaccag cagctgtgcc ccagcagctt ctaatgccaa tgcatcgctg
     8161 agcagttgat cgcccgcttc atcgccgcgc ccctggactt tgtagccacc gaaaatattc
     8221 actgactgtg gggttaggcc taaatgacca caaactggaa cggcacgttc ggtcagcatt
     8281 tgtacggttt ccaccagcca ctcaccgcct tcaattttga ccatgttggc tccggcacgc
     8341 attaccgttg cggcgttttc aaaggcttgt tccggcgtgg catacgccat aaatggcagg
     8401 tcagccagca gaaggcagtt tggcgcgccg cgacgcacag cggcggtgtg ataggcgata
     8461 tcggcaacgg taaccggtag cgtggagtca tgcccctgaa ccgtcatgcc cagtgaatcg
     8521 cctaccagca tgacgttaag tccttcttcg gcaaacaatt ttgcgaagct atagtcgtaa
     8581 gcggtgatgg ttgcgaaacg ttttttgtcc tgcttgcatt tctgcaatga agcaatcgtg
     8641 gttggtttca tagcgtatcc tgataaatga tgttgtgctg tctcgcattt tatcagtcac
     8701 attggtgggg gcaatgattt atccgtatcg caccgccatt gtctggcggt gcaatagtgt
     8761 tatcaggggt aggtaacctg aaaagtgctg gtggctttaa aatcccccgg ttctatagca
     8821 atattaccgt cttgttttaa tgtagcctgg aaatgaagag gttgtgaggt accgtttcct
     8881 ttatccgggt aaattcctcc ggtagtgtca tcttctgttt cgtaatcttt ataaacagac
     8941 gttgaatcat ttggtttcag taccatttgt gcacttttag tgttttttaa accttcaatc
     9001 agtacaccaa caccttttgc tgcgttattt cctgtgagtg tgttagccag aagttctttg
     9061 cttacactgc ccaccttgtt tgacataagt tttgtttcta tattgcgaac acgaatacag
     9121 ttctgtagcg tgatatcaaa aggaaccgct gttgcgccat tttttatttg cgctggggta
     9181 taatctccca ttttaactgt tgaaccgctt acagatgggc cagtaagtgc agcagtaaaa
     9241 cacgttgggt tggataacgt catattatcc aggtcaaaat ctattttaag gaaatagctg
     9301 tgatcgttga taccgccacc aagaccatat cctttgaatg agtagagata accacccggc
     9361 ttagataatg ttaccttgtt tgtctttgtg gggtcaaagt tggtatcggt ataaaattcc
     9421 accgtgagcg actgccaaac tccacctacg gcccagtatt tattagatga gtttgcattg
     9481 ttacaagacc aatatagagt ggcttcactt tcggggatcc accgaaaata ttgagttgga
     9541 gaatcgccga tatacatacc tgatgaactg atattggttg tggtgtatga tgaccaaatg
     9601 tttgatatct ttaatgtata atacaggcct gggacagatg tttcaaatag tttatgcccg
     9661 ccataacgtt tacccgaatc aaccatttga ttttcaattg ccataggctc accgcttgca
     9721 gtgttacttc tgttgcagta aaggtaacct ccacctgaag cgctatttaa ctgagaccag
     9781 tataatgaca cacctgttgg tccgagttgg gttggtttat aaaaaactaa gttatttgtg
     9841 cttgttgaat acctgaattt gacagtaatg tctgcactgg ggcctgtttc tctccacttt
     9901 tggtctcccg gacccactcc gtaatcattc ccaacaagat agctgacgtt atcatcggtg
     9961 ccggcacttg ctgtataaca acaagagcat agtgctaaaa aaagaaagta tctgaagaat
    10021 atcttcattt ttactccatt gtcatgctgc aagataatgc ttgcagcact ttgtctgtta
    10081 ttcgtagtaa atatccacta acacatggct tataagttgg ccagaggtga cattcagcgc
    10141 tggatcgatt ttttgcattc gagtgctaaa tttccaggat gaattgttta gtgctgatga
    10201 catgaccgga taaacggaac gtgagctacc agttacgcca agaacgttga aggtatttgc
    10261 cgggtcctga atagagaata taactacccc aacattttta gccccggtgt tgtcttcatt
    10321 ggcgaatatt tgactggaac ctgtcgccat tacgccactg agtgcgcgaa aatcaatatg
    10381 taactgattt aacacattcc ctgtgccctg aagtgtgcag tcctgtactg taatagtgaa
    10441 ctcttttcca ccggggtgat aattttcggc tgaaatgttt ccggtaaaat cgcccacgcc
    10501 aacgacacct aaatcaatat ttccgttgtt gctgattcca ctttggcagg tgttattttt
    10561 tatatttgca acaatgtcaa catcctgtcc tgcaaccaca gggaaagtgg tgctgaatag
    10621 caaagacaga agcagaattg attttttcat cagattacgt tgagtggggc gcatatttag
    10681 ttcctgaatc ggtctgcctg ttagttataa gtcacagtaa atgttgccgg agcggtgaaa
    10741 tcacccgcgg tgacatctgt aacggtttta tctttcgcga ccaccaaaac agcactcatc
    10801 gggtaatcga caacatcacc tcccgtcgca ccagcaatac tgacttcttc tgttgcgggg
    10861 gtgacgcaac tgaattgttt tgcgccgttt ttacctggtg tggacgtact ccagacctca
    10921 acggcgactg ccgctgcttt tgctgttgcc gttgatgcgt tagcaaatcc agggccgtta
    10981 ttagaattac cttcgcataa ggctcgtggg gtcaacttta ttttggcctt tttcccggga
    11041 atccccgcac agtctttgaa ctgcaatttg aaggttttaa ttttagtttc ggcattaatt
    11101 tcagaaatat atacatcacc gaaattgatg gtggacagtt tagctgtacc gtcatcagtc
    11161 aacgttgatg tacaggtacc catttctatg tctgtagtaa cagtcaggcc aatactatca
    11221 gtggccgcat gtgaggaaat ggttaacagt gatgaagcca gaaatagact acccgataat
    11281 ccataacata atttttttaa tatcatcatt gtcatccttg ttattgatag atgaaattga
    11341 acgttgcctg tgcgcgaaat ttcccgggta tgccctgtcc tggggttgtt tcgcgcagtg
    11401 cgacagtcat ttccagacca ctggtgctaa tttctgtggt tttccagcgg atgtattccg
    11461 cgctattggg tttcagaaac gtatcgcccg aagtctctaa tcttttaaat cccataccaa
    11521 tattactggt tgttgaggag gagtcagaag attgtggaat aatcagcgat gtactaccag
    11581 actgatttcc agtcattgtg gtatcaatcc agctaattcc actgctacac ccactggcga
    11641 ctaatttgaa gttggcctca gattgtgacg ctttattgac tatcttatcc agtcccatct
    11701 tggggattat cagggtataa ctatcattgc cattatcaac gacgtttgaa ccattcagcg
    11761 atgtcagggt gatgttacat gttgtcgctt tcacggtagc ggtaaaatcg acattaacat
    11821 ccgttgcaaa tacagatggc gataataata gccccatgag tattgctatt ttatgtggtg
    11881 tggtttttat cattggaagt cccttgtggt tactgtatct gacaacgaag tccgttcaga
    11941 ataatagatt gcgctatttt ttctgatgtc gtgttttgtt ggtaatgagc aaaacaactg
    12001 actggtttgc cctcttcaat ccaggtaatt cgtatatttc cttcctgccc gataccgcga
    12061 acaaaggcct gaccaccctg acctacgttg ccgatgatat tgttttgttc atcataaata
    12121 tccgctgcaa atggaatatt tttaccatcg cttcgcacga tattcataat tgccgattgt
    12181 ccctgaactg tttcaaaatc agcaaagacg acggctccct ggcgcgggac ggcgacagta
    12241 ctggtgcttt tcaattcaac atcgttttcc agatcgttaa tgtccagtgc gatacggttt
    12301 tcatggtatg gcgaaagggc gctggtcaca ccataacccc agcggtctac ggtgctgttt
    12361 ccgtagttaa tacgagcgcc ttttgctcct ggcgcctgaa tgacagccag agtgtcggaa
    12421 tcactaaaac tgtcattact gaacgtcagc ccgccgctat gtaacacaaa tccaccatca
    12481 gtattgagtg agaattggcg actattatca ctgcttgctg aggctgaacc tgacagagtt
    12541 ccccacggcg attcataact ggcataaccg ccgatataac tcaggtcatc actggattta
    12601 ttcatcatat aacccgtgtt tacgctgtag ctgatgcgat tggtatcgct gtaaccacta
    12661 ctgctgatat tgagttggtt attaccctta aagtcgctgt ttaattgtgt atcaatactc
    12721 tggaagccgg agctgcggtg ttccgtaccc agtaaatttt ctattggaat cgtgaagctg
    12781 agataaatgc tatcgtcggt ttgaccatct tcattcaacg aacgttgagc actgatactg
    12841 taactgcccc aggacgcact attgctataa ccaatagagt aattagtact gttttgcccg
    12901 gaggcccagt aatctgacca gcttcccgag agataaaatg aaccgtaatc ctttttctca
    12961 aatttcagcg gttggttgat actgaccgtg acctgatttt tcatgcgcga gtaattgcgc
    13021 atggatttag gctcaagatc ttgctcagga tgttctactt catcaatcag cgtcagcgca
    13081 tcattgaggc ccagataatg ctgggtggag tagcgatagg ccgcgatatt taatgatgta
    13141 ctggtatttt caaataactt gttccaggaa atacggtagc tttgtccttg ataggtttta
    13201 tcgtccggaa tgctgacatt tgagtgagta acatcgacag aaaaagcccc cacgggggta
    13261 ttcataccaa gccccagcaa tccggcggta tagttattat cagttagctg aataccggta
    13321 tatcctgtca ggtagttatt caaaccatag tagtagctgg cttgaaataa attaggctca
    13381 tcctggatac tgtcttttaa aacctgaccg gcgctaatat cccagcgacc aacgccaggg
    13441 cgcagcattt gcacaacaga tgaaaatggc tgggagaacg tccgttttgt gccatcagct
    13501 tcttcgatag tcacaatgag atcactgcca taacctgacg ggctaagatc atcaatggca
    13561 aaggcccctg gcggcaccgt cgtttcatag attttataac cattttgcat aacggttact
    13621 ttggcattgg tattcgctac gccatgaatg attggcgcaa aactggctaa taccggaggc
    13681 agcattcgac tgtcactgta taagcgaata ccgcgaatac tgactgaatc gaaggtttcg
    13741 ccggttgtgt aagattcacc aataaccagt tgtgaacgta gtgacgcaag atcgcgttgt
    13801 aaatagcgat tctgaaaatc gtaatcgcta tggacattgg tcatccagtt gtagttaccc
    13861 gaggcacgta accgccaggc acctaagttt atcccaccgt tgaatgccgc gtagatactg
    13921 tcatttgttc tgcctggact ttcactgtga tatccgttga ggttgtagga aagcatggca
    13981 gcattaatgc cattttccca caaagaggga tcaacatagt tttgataatt cttcataatc
    14041 catgcctggg ggacgtcgat atccaggcgt tgatcgttaa catcgtaacg aatggatgat
    14101 tgagggatca tttcagccag attaaggcaa tcaccaagat cttcgtctcg cttaagcaaa
    14161 atggcttttt cgctattctt gtctggtgaa ctgatatgaa attgcaataa atttttctgc
    14221 gtaatgcagg cctgagcgtt ctttttacct tcaataacag taaaagcaat gctttggctc
    14281 atgataggtt gatcgttaac atacacactg acatcgtaaa tgcctggtag tgtcgggttg
    14341 ccttctgtat accgactaag gtcgatattt gaagcatcct ttcccatcag aaatgtatgg
    14401 tcatattcga cgagttcagc gcataaagca ctgttgctat acagcagcgc gcaaaacgtg
    14461 gcgatgcggg taaggtgatt atgatatttt ttagtggatt taatagtcac gttctgcttc
    14521 ctgctgtggt atatccttgc cagattaaag gttggcgttg ccttcaatag agccgccaaa
    14581 atcattaatg gcgaaaaaat gcacttttgc agagcttgtt ctgccactca tcccatttac
    14641 cttgatgacg tcgttactga acggtgcaat cattttcaca tccaggggat agcgttttcc
    14701 gttggcttct aaatcaccac tgctaaaaga gacatagtag ggcgtagggt tatcaacgcg
    14761 taatgccgct ttgccttcag atgttgccca ggaccacttc agagccaatg gcgcatcaga
    14821 tggaattcca ctcaatcctt ccgggcgata gaacagtttt atacgtgtac gaaatgccag
    14881 ttgtagcagg ctttgatttg ctgcttttgc tgcatcgggt tttggtggaa cttccagtac
    14941 gttaaaccag aacacacttt ctctgtcttt tggcagagca ctactggcgg tgtacatcag
    15001 tttgattgtt tggccacgtt tagcatcaat acgtgatact ggtggcgtag cggtaaaggg
    15061 gacagtaata ctgcctggtt cagcgttgtc atctccagta tctaaccaac tctgaaccag
    15121 caatgggtta ttgcctttat tttccagacg cacattgacg cttttttgtt cgcttttata
    15181 aactacgcgt gtacctgaaa ttacgatgtc tgcaatagct gatgagctaa aagccataca
    15241 gctcgctaag cacaatgctg cggtatgttt agtattaaaa aacataaacc gttcctcaca
    15301 gagttaatta aaacagggaa taacgttcaa aaataaatat gtttgatgtt attccctata
    15361 tatattcacc caatcaattt aggtggctga tatttactgg taagtaatgg tatatgctga
    15421 gttagttttt acataaccgg cagttgctgc tttgcttgca tcagcacgaa cataggatgc
    15481 tttaaaatcg taggttgcag ttttagacgt tgcatccaga gttttgctgt aaacatcacc
    15541 cgggttatta acctgaacct gcttaatagt ggaaccttca atattatgca gggcaatatt
    15601 cacaccatca gatggattat tgatagacat atcgttattc agagtacctt tgctattgct
    15661 aaagaacacg gaaccgaaag tcatttttgc agtagtaccc ggagccgggt tagctttgct
    15721 gcagtcaata gtaatgctga atggcttagc tttagcacct acggtggtat ccagtacacc
    15781 atcggtgatt tcagcaacag tagcggtttg cagcaggatc aaaccatctt tgttaccgcc
    15841 atcaacacga gcttcacagg tgttatcaac gaccaggccg ctaatattta actgaccacc
    15901 gtccatatcg ttagcagcaa atgcagtacc agcagccatt gccaacataa taccagacag
    15961 cgcaaaacct aattttttag acataaaaat ccttgaataa tattccatag agagataacc
    16021 gcaccgcatg acgataagag tctgctgcaa tgctgcgtaa caaaatcgtt actgtgttaa
    16081 tgatgtttgt ctgttgttta ccaactatta cattcattca tcatcgggat ggct
//
LOCUS       DAZUCC010000112        16079 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZUCC010000112 DAZUCC010000000
VERSION     DAZUCC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.112"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            289..1188
                     /gene="yegS"
                     /locus_tag="ACHY09_003007"
     CDS             289..1188
                     /gene="yegS"
                     /locus_tag="ACHY09_003007"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFS3885955.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1270..2043)
                     /locus_tag="ACHY09_003008"
     CDS             complement(1270..2043)
                     /locus_tag="ACHY09_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS3885956.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2149..3189)
                     /gene="gatD"
                     /locus_tag="ACHY09_003009"
     CDS             complement(2149..3189)
                     /gene="gatD"
                     /locus_tag="ACHY09_003009"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS3885957.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDVVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3237..4592)
                     /locus_tag="ACHY09_003010"
     CDS             complement(3237..4592)
                     /locus_tag="ACHY09_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFS3885958.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(4596..4880)
                     /gene="gatB"
                     /locus_tag="ACHY09_003011"
     CDS             complement(4596..4880)
                     /gene="gatB"
                     /locus_tag="ACHY09_003011"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFS3885959.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4911..5363)
                     /gene="gatA"
                     /locus_tag="ACHY09_003012"
     CDS             complement(4911..5363)
                     /gene="gatA"
                     /locus_tag="ACHY09_003012"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFS3885960.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5373..6635)
                     /gene="gatZ"
                     /locus_tag="ACHY09_003013"
     CDS             complement(5373..6635)
                     /gene="gatZ"
                     /locus_tag="ACHY09_003013"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFS3885961.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMFDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6664..7518)
                     /gene="gatY"
                     /locus_tag="ACHY09_003014"
     CDS             complement(6664..7518)
                     /gene="gatY"
                     /locus_tag="ACHY09_003014"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFS3885962.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7747..8799)
                     /gene="fbaB"
                     /locus_tag="ACHY09_003015"
     CDS             complement(7747..8799)
                     /gene="fbaB"
                     /locus_tag="ACHY09_003015"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFS3885963.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9056..10333
                     /locus_tag="ACHY09_003016"
     CDS             9056..10333
                     /locus_tag="ACHY09_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFS3885964.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10330..11334
                     /locus_tag="ACHY09_003017"
     CDS             10330..11334
                     /locus_tag="ACHY09_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFS3885965.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAYFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            11331..12296
                     /locus_tag="ACHY09_003018"
     CDS             11331..12296
                     /locus_tag="ACHY09_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFS3885966.1"
                     /translation="MSGARLHTLLPELATRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAATLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12270..13016)
                     /locus_tag="ACHY09_003019"
     CDS             complement(12270..13016)
                     /locus_tag="ACHY09_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS3885967.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13068..13886)
                     /locus_tag="ACHY09_003020"
     CDS             complement(13068..13886)
                     /locus_tag="ACHY09_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFS3885968.1"
                     /translation="MQLRITSRKKFTALLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(13951..14751)
                     /gene="thiD"
                     /locus_tag="ACHY09_003021"
     CDS             complement(13951..14751)
                     /gene="thiD"
                     /locus_tag="ACHY09_003021"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS3885969.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWTDTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14748..15536)
                     /gene="thiM"
                     /locus_tag="ACHY09_003022"
     CDS             complement(14748..15536)
                     /gene="thiM"
                     /locus_tag="ACHY09_003022"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFS3885970.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15571..15667)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15759..16031)
                     /gene="rcnR"
                     /locus_tag="ACHY09_003023"
     CDS             complement(15759..16031)
                     /gene="rcnR"
                     /locus_tag="ACHY09_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFS3885971.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
BASE COUNT         3943 a         4200 c         3882 g         4054 t
ORIGIN      
        1 acatacacgg atgcccttcc aggtaacccc atgggggggt taatatattt ttaatagtat
       61 attgaagatg ccactgttta gttgaatatt aggtatatgt tcttttttga aatttaccgg
      121 tggctaccgt taatattcgc tgttcccatt gcaagctcct ggtggtaacc actgaatcct
      181 ccatacttga actgactttt tatcctccga ctttcatcct gttctgactc caccttttct
      241 tttctgctct acactatcta cagtccaatc ataaaggcac atacgatcat ggcagaattt
      301 cccgccagct tactgattct taatggcaaa agtactgaca atctaccctt gcgcgaagca
      361 attatgctgt tgcgtgagga aggaatgacg atccatgtgc gggtcacctg ggagaaaggc
      421 gatgccgcac gatatgtaga ggaggcacgg aagttgggcg tcgcaacggt gattgccggt
      481 ggtggcgatg gcaccattaa tgaagtttct acggcgttga ttcagtgtga gggggatgac
      541 atacccgcgc tgggaatttt accattagga accgccaatg attttgccac cagtgtaggg
      601 attcctgagg cactggataa ggcgctgaaa ctggcaattg ccggtaacgc cattgcgata
      661 gatatggcgc aggtcaataa acaaacctgt tttattaata tggcgacagg cgggtttggg
      721 acgcgtatta ccacagaaac gccggaaaaa ttaaaagccg cgctgggtgg cgtctcttac
      781 atcattcatg gcttaatgcg catggacact ctgcaaccgg accgttgtga aatccgcggt
      841 gaaaactttc actggcaagg tgacgccctg gtcattggta ttggtaacgg gcgtcaggcc
      901 ggtggcggtc aacaattgtg cccgaacgcg ttaattaacg atggcttgct gcaactgcgc
      961 atttttaccg gcgatgaaat tattccgact ctcgtatcaa ccttaaaatc tgacgaagat
     1021 aacccgaata ttatcgaagg agcttcgtcg tggtttgata tacaagcccc acacgaaatc
     1081 acttttaatc ttgatggcga accgttgagt gggcaaaact tccatattga aatacttccg
     1141 gcggcgttgc gttgtcgatt accaccagat tgtccattgt tgcgttaatc aaactattct
     1201 ctgttgaacc agagagaatc cgtaatgccg gatgcggtgt aaacacctta tccggcatac
     1261 agaacaatac tacaccgtaa ttaattttac ccgtgtcttc tgcaatgcca gtttatccac
     1321 ctcagacaca gcatcatcgg taataataat gtcaattttt tcgattggca gtacctgatt
     1381 aaaaccgcga cgattaaatt tcgatgaatc gagaacggca atcactttat tcgccgctgt
     1441 caccatcgca ccgctaacgg aataaccttc attaaaggtc gtaataccat taaccgcatc
     1501 aataccatca gcaccgacaa acattaaatc ggcattaata tcctgcaatg aacgctcagc
     1561 aatggaacca tgcatcgagc gcgttttatg gcgcaccgta ccaccacaaa ccaccaaagt
     1621 gatgtcttta ttttcggaaa gggcaaacgc agccgggaga ctgttggtaa tcaccgtgat
     1681 atttttggcg gtcattaatc cttcggcgat aagcattgtc gtacttccgc tgtcgagaat
     1741 aacggtcatc ccttcgtgga tcattttgac tgccgcctgc gctattctgt ttttcggcgc
     1801 gctggcgagc tgaaatcgct ctttaaaccc gacttcctgg gtctcggttt cgctattacc
     1861 ggacattatt ttcgccgcac cgccatgaaa gcgcgtaacc acgccttttt gttcgagaaa
     1921 gcgcaaatcg gcacggattg tcgcttccga ggcagcaaat actcccgcca gatcctgaac
     1981 aagcacggtt ccctgttcat tcactaattg gatgatctta ttccttcgct cgaatgaatt
     2041 catcgtcatt tacccatatt caattgtggc tagtgtaaac gaagggggac cattggtgaa
     2101 tgatctgttt tgcacaatac cgggcctgca tcacgctggc ccgcggtttc agggaatgag
     2161 caacactttg cccggcatgg cattacgagc gatgtcacgc accgcctggg cgaagctttc
     2221 aaagcttcca cggtgagcga ttaatggctc caggcttaac ttacgttctg tcagcaaccg
     2281 gctcgccgtt tcccactcct gccccggcca agggctggag tagttcatcc aactgccgat
     2341 aaccgtcagc tctttacgca atattttgcc aaacgttgca gatgttaaat gcagatcctg
     2401 atgcaacgtg cccaccagcg ccagttgggc atgaggaccg gcaatctcta ccgccagttc
     2461 gacggtttgc gggacgccag ccgtctcgag gataagctga ttaaagcgca gctcgcgtaa
     2521 aacgccctgt atttgcggcg cgctcatttc acggctgtta aatgtttgca tcgctccgaa
     2581 agattttgcc agtgccagtt tttctgagct aatgtcgata gccgtcacac tctttgctcc
     2641 cagcgcgaca gcgcactgaa ttgccagcag gccaatggtt ccggcaccaa taataataac
     2701 gtttttattc tcacatcctt gcgctaaatg gaacgcatgc aggccaacgg taatcggttc
     2761 aataaaagcg ccatcctcaa taggcatatc cgcaggtagt gcaaagacat tgtttcgctt
     2821 aacgacaata tattcggcaa atccaccatc acgccgcgag ccaataaaat catattttgc
     2881 gcactgggaa taaaaccctt tcaaacactc tggacaagta aaacagggta ataacggcac
     2941 acaggcaacc acatcgccag ggtgtaaatc atcaacaccg gatcccaccg catcaatata
     3001 gccgctaaat tcatgaccta acgttattgg ataataatgt gcaccatttt taaatatcct
     3061 gggtaaatcg gaaccacata agcccgagct ggcaattttt acccgcacct catcctgatg
     3121 tttaatttca ggaatgacgc tttctgcaac gcgcacgata ccatcagtat cattcaccac
     3181 tgatttcata aaaactcctg attattaagg gggataacct ccccctcagg taaaaattat
     3241 tctgcgagaa cggctttctc ttgtttaata aagccacgcg ctctacgcca ggtcatgaaa
     3301 ataccggtaa gataaatggc accgataata atgaaaccgg gaatattttg cggggagaaa
     3361 acctgaatca gtaaccaggt aatgggagaa ccgccctgat ccattgaagc caccatgccc
     3421 ccggctttta acgccccagc attagccgcc agttgggtgt gcaaaccaat agtttgcgtc
     3481 gcgatccaca gggtgatgct cataatgatg acacccgaga ttaaggtgcg gaacagattt
     3541 ccacgatgca ccgcgaccgc catcgccaca aagaagccga tggtggcaag gtcgccaaac
     3601 ggcagcacct gattacccgg cacacaaaca gcaattaaaa tggtgagtgg aataaaaatc
     3661 aggcttgccg ataccaccgc cgtatgtccc agcagcaacg ccggatcaag gccaatcagg
     3721 aactcctgac cgccgaactt cgcctgtaaa cgactacggg cctgcttagc gatgggcgtt
     3781 aaaccatcca tgatgggttt aatcacccga ggcatcagca gcataactgc cgccgttttt
     3841 accgccagct gcaatacacc tttgacatcg taacccgcga ggatgccgat aatcagcccc
     3901 atcacaaaac ccacggtgac cggttcgcca aaagggccaa atttgcgctg aatatcatcg
     3961 gcgctaaatt taattcggtt aacgcctggg attttctcga tgatagcatc gaccagcacc
     4021 gcaatcggcc ccatatacgc cgacgtaccg tgcggaatag caatgccttc cagctcaaag
     4081 aaatttcggg tatcgcgggc aaaccagtcg ccgagcttat aaacaaacgc cgcgtgaatt
     4141 accacacccg ccatccctat catccatgaa ccggttgcca gatgcagcaa cgcgccggtg
     4201 aaggtcatat gccagatatt ccagatatca acatttacca cccgcgtcat acgggtcagt
     4261 aacatcgcca cgttaaccag aatcgcaatc ggaatcgcca ccagcgcaat ttgcgacgcc
     4321 caggtcatgg gtgaagagcc cggccagccg acatcgacca catgcagatt caggtcgaaa
     4381 ttttccgcca tcgctttcgc cgccgggcca atggaatcca gcattaagcc aatcacaagg
     4441 ccaataccaa caaagccaat cccgatatgc agacctgctt taaagcaatc gcctgccttc
     4501 atgcctaata ttttagaaaa aataatgatg acaatcggca gcatcaccgt agggccgagg
     4561 tcgagaatat aacgcatgac ttctgaaaac ataggtcacc cctgtaagat agtcagaatt
     4621 ttattttgta atgcttcgat accgacacca gaaacaaaag gcatgccgtg aactaacgga
     4681 atatcgccaa aacttcgatc caccctggca gtggtgcata tcaaatgcac gccatccata
     4741 taggtttcta tttcattaac ccgacactgg attaattcaa caggaatatt atgactctga
     4801 cacaactctt taatttcttc cgccgccatc gtagaggtcg caaccgcgcc tccgcaagcg
     4861 acaataatct tgcgtttcat aaatagtcct ttttattgtt acagagggat ttattcgtct
     4921 gaatctaaaa catactttgt gaagtattcc tttaactggg tttcaggaag agtgattagt
     4981 gtctcgacga tctcgggctg ttgtaacttg ccaaataaac agcgtaaaag tttcaattgc
     5041 tgctgcggat tttccacaat taacgcaatc accaacgata ccgccacgtc gttatcatca
     5101 tccgcttgct gaaaatgaac tttatttgtt ggccttaaca gataaatggc tgacgactta
     5161 gcatgaatcg cctcacaatg cggtattgca atggcgtgct gctcaagcat tatcccggta
     5221 gggaattctg cttctctggc aattaatgcc tgtggccagg tatcatgaac cacacctttc
     5281 gcgagcatct cattaccgat atgggttaaa acttcgctac gatcgacaaa agaaattccg
     5341 ctacgaacaa acaggttagt catataccgt ccttattccg cacagccgta gcgataggcg
     5401 cgcaaaacat cataaatttt atccataatc aactgatgcg gtattgctga taattccccg
     5461 gactgaatgc gttcaaattg tttaggaaga tactgactaa tcatgcccag agggatttcc
     5521 atgccttgca gattcaccat catcgtttcg acgctatttt taatccggct atgcggccag
     5581 taataacgaa tacgatccga caggctgtaa cgaatatcca gtaatgaatc gttaaaaccc
     5641 gtacgataat attttttcca gtattgcggt tcgtcaaaca tcacttcttc aattaccgcc
     5701 aggcaaccgc tgcgattttc tggggcgata agttcctgct caatttgcgc cagcgcaaat
     5761 atcgcctcac gtaaagcaaa ggttaatgcc ggaccgactt tcaaaattgc aaaatgatcg
     5821 cgaactaatt cccgataggc cgtctgagtc tggtaatcgg tggaatgcgc ttcataaacc
     5881 attttggttt tttctatcca ttgcgccagc gcctgagctt cttgcgcctg ataatgaata
     5941 atattgctat ggtcaaattc cacacccggc tgcaccacga tggcaatcac gcgtgttaac
     6001 gcctctgcca gaccgcgggc aataaacgct ttttgatgcg tgcgtaaagt attggcggca
     6061 tcttcaacct gggtgatatg tactgactga atggcgctgg cctcaccgcc cggaaccgga
     6121 acttcggtgc caatgacata gctcagttgc tcacgctggc aatctgtcgc cacactttcc
     6181 gcagcaaagc aaagcacagc agctcgttcc gcaaccgttt ctggggctaa cggtatagga
     6241 tcccccgcgc aagacattga cgcatcaaga tgaattttac tgaagccggc acgaacatat
     6301 gcctttacca gttcgacgga tttttccatc gccgcatcca cattttcttg ctgccagcag
     6361 tttggtccca gatggtcgcc accgagaata atacgctcac gagcaaatcc gactttatcg
     6421 gcaatcgcaa aaacaaattc gcgaaagtct gccggtgtca ttccggtata accgccaaat
     6481 tgattgacct ggtttgacgt tgcttcaatc agcactttac gcgtgctgtt gcgatcaaat
     6541 gccagcgccg cttcgataac caacggatgt gcagaacaga ctgaacatat gccaatatgt
     6601 tcaccagctt tatgccgggc aattaacgtt ttcatgtttt ttccttgtta aatggcaggt
     6661 gcgttatgcc ctgccctcgc agccacaatc ggcaatcact ttgctcacca catcgcgcat
     6721 tgcggattta gccgactgca aataatcccg gggatcggtc gcttcggggt gctctgtcag
     6781 gtaatttttt aacgcctgcg agaaggcatt ttttagctct gttgcaacgt tgattttgca
     6841 tatccccagt ttgatggttt gctgaatatc cttagttgat aatcctgacg cgccatgcag
     6901 caccagcggt aagttcaccc actggcgaat gttctccagt cgagaaaaat caagcgccgg
     6961 tgcgctggca tacataccgt gagccgtgcc gatggcgacc gccagggaat caattccggt
     7021 tgcctcggca aattcacgcg cctgaacagg gttggtgtac aacgcatcgg cttcattgac
     7081 ttgcacatca tcttcctggc cgccaagttg ccccagctcc gcttcgacgc tgacatcaaa
     7141 gcgatggcaa aaatccacca cctctttgac ccgtgaaata ttttgcgcaa aaggcaaatg
     7201 cgaggcgtca atcatgactg agcgcacgcc agaacgaacc ttctgagcga tatcgtcaaa
     7261 tttcgtgtga tggtcgagat gaattgccag tggatggtga tattgcttcg ccatcgcgct
     7321 gaccagcgcc aacagatttt ctgtaccggc atgagtaaat gtgccaggcg ttccggcgat
     7381 gatgaccggc gcatgcaggt tggcagcggt ttctaccacc acttgcatcg tttcgagatt
     7441 gtgaatattg aatgccggaa ccgcataacc gccgcgctgt gcgttgttca gcatctgctt
     7501 tgtcgatacc acgtacattt tcatatcctg tcgtttgttt tcgatttcaa aatataatga
     7561 aatgatttgt ttttaaatgt cgcgataacg atcacaaaaa cgacaatatg aaaatcaatc
     7621 caggaatgaa aggcaaaaaa aacggcctcc cgggggaagc cgtaacaaag tacgcgtggt
     7681 tttatgccgg atgtggcgtt aacgcctttt ccggcctacg gggtagtgct gttcgtatga
     7741 agcgcatcag gcgatagtaa ttttgctatc gagataaacg tcctgcacgg cgttaatcag
     7801 tttcacgccg tcagccatcg atttcttgaa cgccttacgt ccaagaatca gccccattcc
     7861 gcctgcgcgt ttgttgataa ccgcagtacg cactgcatcg ctgaggtcag tttcaccgcc
     7921 tgcagcaccg ccggagttta tcaacccggc ccggcccata tagcagttag ctaactgata
     7981 acgcaccagg tcaatcgggt tttcactggt cagtttgctg tagactcgat cgtcggtata
     8041 accgtaatta atcgctttat agccgccgtt attttccgcc attttttgtt tgacgatatc
     8101 tgcacctatg gttgccgcca gatggttcgc ctgaccagtc aggtcggcgg aaacatggta
     8161 atcaacgcca tctttcttaa aggcggagtt acgcaaatag gcccacagca ctgtcaccat
     8221 gcccagctcg tgcgcacgtt caaaagccgc agaaatttct tcaatctggc gacgtgactc
     8281 ttccgagcca aaatagatag tcgcaccaac cgccaccgcg cccatattga acgcctgctc
     8341 cacgctggca tacagcgttt gatcgtaggt attcgggtaa cttagcgtct cgttgtgatt
     8401 aagtttgacg aggaatggaa tgcgatgcgc gtaacgccgc gataccgatg ccagcacgcc
     8461 gtaagttgac gccacacagt tacagcccgc ttcgatcgcc agttcaacaa tgtttttcgg
     8521 gtcaaagtag agcgggttag cggcaaatga agctccggca gagtgctcaa cgccctggtc
     8581 aaccggcaga atagaaagat atcctgtgcc agccagacgc ccggtgttgt acaacgtctg
     8641 catattacgt aacaccgctg gcgggcgatt attgtcaatc atcacgcggt ctacgtagtc
     8701 atgtccgggg agataaagct ggtcagaagg aatagtcata caacggtgct gtaaaaggtt
     8761 gtcggcgtct ttgccaagca actgcgcaat atctgtcatt actatgctcc cgtaaattcc
     8821 gattggatat cggctatgga ttttcctggc ccgccttttg cgggcaatca taatcctggt
     8881 cgttacaggc gagtttttcc atcttttgga tgttttttca gcgttttctg ttggcacgat
     8941 tcatcagaaa aaatgttatc gcggtcaagt tttcaccgca aaggtattta aaaggtatta
     9001 ttaaatggta ttgtcatcgc gtaccttaca ttacctgtca tgaaggaatt aaaagatgaa
     9061 aacaacagca aagctgtcgt tcatgatgtt tgttgaatgg tttatctggg gcgcgtggtt
     9121 tgtgccattg tggttgtggt taagtaaaag cggttttagt gccggagaaa ttggctggtc
     9181 gtatgcctgt accgccattg cggcgatcct gtcgccaatt ctggttggct ccatcactga
     9241 tcgctttttc tcggcgcaga aagtgctggc ggtattgatg ttcgctggtg cggcgctgat
     9301 gtatttcgct gcgcaacaga ccacttttgc cgggttcttc ccgttactgc tggcctactc
     9361 gctaacctat atgccgacca ttgcgctgac taacagtatc gcttttgcca acgtgccgga
     9421 tgtggagcgt gatttcccac gcattcgtgt gatgggcact atcggctgga ttgcctccgg
     9481 tctggcatgt ggatttttgc cgcaaatgct gggttatgcc gatatctctc cgactaacat
     9541 cccgctgctg attaccgccg gaagttctgc tctgctcggt gtgtttgcgt ttttcctgcc
     9601 cgacacgccg ccaaaaagca ccggcaaaat ggacattaaa gtcatgctcg gcctggatgc
     9661 gctgatcctg ctgcgcgata aaaacttcct cgtctttttc ttctgttcat tcctgtttgc
     9721 gatgccactg gcgttctatt acatctttgc caacggttat ctgacagaag tcggcatgaa
     9781 aaacgccacc ggctggatga cgctcggcca gttctctgaa atcttcttta tgctggcatt
     9841 gccgtttttc actaaacgct ttggtatcaa aaaggtattg ttgcttggtc tggtcaccgc
     9901 tgcgatccgc tatggcttct ttatttacgg tagtgcggat gaatatttca cctacgcgtt
     9961 actgttcctc ggcattttgc tgcacggcgt cagttacgat ttttactacg ttaccgctta
    10021 catctatgtc gataaaaaag cccccgtgca tatgcgtacc gccgcgcagg ggctgatcac
    10081 gctctgctgc cagggcttcg gcagtttgct cggctatcgt cttggcggtg tgatgatgga
    10141 aaagatgttc gcttatcagg aaccggtaaa cggactgact ttcaactggt ccgggatgtg
    10201 gactttcggc gcggtgatga ttgccattat cgccgtgctg ttcatgattt ttttccgcga
    10261 atccgacaac gaaattacgg ctatcaaggt cgatgatcgc gatattgcgt tgacacaagg
    10321 ggaagttaaa tgaaaacaga acgtattctc ggtgctcttt atgggcaggc gttaggggat
    10381 gcgatgggga tgccctccga gctttggccg cgcagtcgcg tcaaagcgta ctttggctgg
    10441 attgaccgtt ttctgcctgg gccaaaggag aataacgcgg cctgttattt taaccgcgcc
    10501 gaattcaccg acgatacctc gatggcgctg tgtctggcgg atgcgttact ggaacgtgaa
    10561 ggcaagatcg atccggatct gattgggcgt aatattctcg actgggcgct gcgtttcgac
    10621 gcctttaaca aaaacgtact aggtccgacc tcgaagattg ctctcaacgc cattcgcgac
    10681 ggtaaacccg ttgctgaact ggaaaacaac ggcgtgacca acggcgcagc gatgcgcgtc
    10741 tcgccattag gttgtttgct tccggcgcgt gatgttgatt cctttattga tgatgtggcg
    10801 ctggcctcca gcccgacaca taaatccgat ctggcggttg cgggggcggt agtcatcgca
    10861 tgggcgattt ctcgtgccat tgacggagaa agctggtcag cgattgttga ttcactgcct
    10921 tcaattgcgc gacatgctca acaaaaacgc atcactacct tcagcgcctc actggcagca
    10981 cgtctggaga ttgcgctgaa aattgtgcgc aatgccgacg gcaccgaatc cgccagcgaa
    11041 cagctttacc aggtcgttgg cgcaggtacc agcactattg agtccgttcc gtgcgccatt
    11101 gcgctggttg aactggcaca aaccgacccg aaccgttgcg ccgtcctgtg cgctaacctt
    11161 ggcggcgaca cagacaccat cggtgctatg gcgacggcaa tttgtggcgc gttgcatggc
    11221 gttaacgcta tcgatcctgc gttaaaggcg gaactggatg cagtaaatca gcttgatttc
    11281 aaccgctatg ccacggcgct ggcgaagtat cgccaacaac gggaggcgat atgagcggcg
    11341 ctcgattaca cacgctgctg cctgaattag ccacgcgtca gccagtgatg gtcgtcggcg
    11401 cggcggtcat tgatgtgatc gccgacgctt atgccctccc ctggcgtggg tgcgatatcg
    11461 aactgaaaca gcagagcgtt aacgttggcg gctgcgcact gaatattgcc gtggcgttaa
    11521 agcgcctcgg catcgaagcg ggtaatgcct tgccgctcgg tcagggcgtg tgggcggaga
    11581 ttattcgcaa ccggatggca aaagagggct taatcagcct gatcgataac gccgaaggtg
    11641 ataacggctg gtgtctggcg ctggttgagc cggatggcga acgcactttt atgtcattca
    11701 gcggtgtgga aaatcagtgg aatcgccagt ggctggcgcg attaaccgtt gcgcctggca
    11761 gcctgctcta tttttccggt tatcaactgg cctcgccctg cggcgaattg ttagtggaat
    11821 ggctggaaga gctgcaagac gtgacgccat ttatcgattt tggcccacgt attggcgata
    11881 tcccggatgc attactggcg cggatcatgg cctgtcgacc tttagtgtca ctcaatcgtc
    11941 aggaggctga gattgccgcc gaacgttttg ctttatccgc agagataaca acacttggca
    12001 agcaatggca ggagaaattt gctgcgacgt tgatcgttcg tctcgataaa gagggcgcat
    12061 ggtatttcag caacgacgct tctggctgca ttccggcatt tccaacgcaa gttgtagaca
    12121 ccattggggc gggcgacagt catgccggcg gcgtacttgc cgggctggcc tctggtctgc
    12181 cactggcgga tgccgtatta ctgggcaatg cagtggcgtc gtgggttgtc gggcatcggg
    12241 gcggtgattg tgcgccaacg cgcgaggaac tactcctcgc acacaaaaac gtatagatcg
    12301 ctgcgacagt ggctgatgct gtactcaata ggccgttgct gttggtcaag cgccacttgc
    12361 ttgatcacca gcaccggtat tttgctatct aactgaatat gtgactgaaa ctcggcatcc
    12421 ggcatccggg cgctaacgcg ggaacgcgta cgttgtgggt aaatgtgctg gctgcggaag
    12481 tagtcataaa gagaaatccc gatggcatca acatcatgaa ttaaatgtgc cggaacccac
    12541 gattcctcaa tcgataccgc ctcttcatcg acatagcgaa tacgcttaag caaaaagaca
    12601 tcacttcctg cttcgaccgc tagctgctgc gcgacttctt cgggacattt cacgacacgc
    12661 ttgttaaccc atagcgtatc gggctttttc ccgcgcaata ccacctgctg agaaaaacct
    12721 cgcgcttctt tcaacgagta ttcgaagatg ttgttgatct gcgtgccgta accacgcgaa
    12781 cgcgtcacca cgccctcttc ttccagcgcc tgcatcgcct tgcgcaccgt aatgcgcgaa
    12841 acgccggtta actgacttaa atcacgctca ccgggcaaaa tattgccatg ctccagcacc
    12901 ccgctgcgca cggcattttt taccgtttcg gcaaacttaa tataaagcgg cgtgttatcc
    12961 gccgctaaaa tacgttcgtt cagttgggca atcagctggg tatgcgcttg ttccatttgt
    13021 ctttttcctg ccagggagtc tggtgggagt atatcactgc ttcttattta aggcgtttct
    13081 ttaatcccat caacgaatcc ctgcaactcc tccaccgtgc cattaaacac attaaaatcc
    13141 accggaccat taatgccatc tacctgtcca cggtcggaat gctgccagaa gcgccaggcc
    13201 attccgtcat tgtccggacg acgttgatag tagtgagcca cccaccacgg atattcatta
    13261 aaatagcccg ccagattggt gtgataaaaa acggctcctg agtaaataat tggctttttt
    13321 cccgtacgtt tttcgaccat ttttagccac tgacttaccc gcttgcgtaa ttcttttgcc
    13381 gataattttc ctcgctcttc tacgtccagt accgcaggga aatcgccttg tgagaaatcc
    13441 accgtttgca gaaataatcg cgcctgaact gaagcggata ctgacgggga gaaataatga
    13501 tacgccccgc gcagcaggcc attttcgcgg cttagttgcc agttacgcga aaaatagggg
    13561 tccaccagct tttcgccttc cgtcgcctta ataaaagcaa actgtaagcg gataccgttg
    13621 tcgcgcattt ttgccacccg ctgccagtcg atccgctcct gccagcgcga aacatctatt
    13681 ccgtgaatgg tgtaactggc aggaatgcga atagcgaaag atttaaccgg gcgataaccg
    13741 tagaagtgga tgtagtcttt aatctgaatt gctgtcgaat agaaaaaatt caccgtctga
    13801 cgcggataaa ttgcaacgat ggaaattagc ccaagagcgc ataataaagc ggtgaatttt
    13861 tttcgactgg tgattcttag ctgcattggt ttcatccctg aatgtcagcg ccaaaggctg
    13921 acaataacca aagcagctat agtacggtgc tcaccaccag gcgtggaagt ggtgaaccgg
    13981 accaataccg tgaccaactt ccagcgtgtc ggcctgggct aatgccgatg aaagccagct
    14041 ttttgcctcc tgtacggtgt cagtccagtt tgtatggcgc gggcgcagtg cagccaacgc
    14101 cgcagagagc gtacaaccag tgccgtgagt gtttttggtc ataatgcgct gtgcggtaaa
    14161 ccgttgttca ccctcgcggg taaacagcca gtccgggctt tgctcatcat ccagatgacc
    14221 acctttcatt agcactgcgc cacagcccat cgccaacagc gatcgccctt gttccagcat
    14281 ttcctgttcg gtgcgcgcgt gtggcgcgtc gagcaaggcg gctgcttcgg gcaagtttgg
    14341 cgttattaat gaaacctgtg gcaataatcg actgcgcagc gtagcaaccg ccgaaggtga
    14401 aagcagcggg tcgccgcttt ttgccagcat aacggtgtcg agtaccacgt tttggatctg
    14461 ataacgttgc aaccgttctg ccaccgcttc aacaatatcg gtttccgcca acataccgat
    14521 tttagtggtg tcgattcgca catcgctgaa caccgaatcg agctgggcgg cgacaaaatc
    14581 aggctcaatg cgatacaccg actgtacacc acgggtattt tgcgccacca gtgcagtaat
    14641 aactgagcaa ccataagcgc caagtgccga gaaggtttta agatcggcct gaatccccgc
    14701 accaccactc ggatcagtac cggcaatcgt cagagcgtta attcgtttca cgcttgcacc
    14761 tcctgcgtca attgccagag cgcatcaagg aaatgtggaa caaaactgcc tggcccctcg
    14821 ctactggcga ctgcgcgttc tccggcttgt ttcatccagt gacaggcaga tgcgatattt
    14881 tccagcgtat cgcctggtag cgcacaacag gcagcgacaa ccgccgataa tgcacagcca
    14941 gttcctacca ctttggtcat taacggatcg ccaccgtgaa tgccaatgat acgatgtcca
    15001 tcggtaacat aatccacctc gccagtgacc acgacgattg cgccagtttc ccgtgccagt
    15061 gtttgtgcag cgggtatcgc gttagctgcg gcatcagtgg tatccactcc ccgtccacca
    15121 ttagcaacgc cagctaatgc catgatttcc gaagcattac cacgtatggc ggttggctta
    15181 tgggacagaa gttccacaca aaaacggcgg cgataatcga gcgcaccaac cgcaaccgga
    15241 tcaagcgtcc agggcgtttg tgatcgtgtt gcttgctcaa cggcagcact catcgcttgc
    15301 gctcgtggct gcgtcagcgt gccaacgtta atcaacaagg cactggcgat agccgcaaac
    15361 tgactggcct cttcggtttc gataaccatc gctggcgatg caccgatcgc cagcaaggta
    15421 ttggcagtaa aagtttgcac cacatcgttg gtcatgcagt gcacaagagg ggaatgttgg
    15481 tgaaaaaggt gtaacgtgtg cgcagattgc gcggaactca gcaggtcgac ttgcatagtt
    15541 tgctcctgcc ataacgtgaa gaagcaatga cctggtggtc cgtgacttcc ctacgctggc
    15601 attatccaga tcaggtgata cgggtatttc tcagccttca caaagaaggg caccccgagt
    15661 cgtttggttg cgataataaa tcgcagagga ggatggtaat gtccagcgca cgcgttgtaa
    15721 acggggaaaa acggcggtat tacaatcgcg ttaataaatt atttgatata agaatccagc
    15781 accttcagaa cgacatccag atcttcttca cgttttagct catccccctg gtgaacgatg
    15841 tgttccgtca gatggccttt aatcacttcc cgcatcagac cgtttaccgc gccacggata
    15901 gcagcaatct gttgtaaaac tgcagcgcat tcatgcggtt cgtcgagcat tttcttgagc
    15961 gccacgacct ggccctgaat cttactggca cgcgctttca gtttctgttt atcacggatt
    16021 gtatgagaca tggcaacacc tggttaacaa gaatatgaaa aatcatagca ctattaatc
//
LOCUS       DAZUCC010000113        15981 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZUCC010000113 DAZUCC010000000
VERSION     DAZUCC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.113"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            173..943
                     /gene="yjjP"
                     /locus_tag="ACHY09_003024"
     CDS             173..943
                     /gene="yjjP"
                     /locus_tag="ACHY09_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFS3885972.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            934..1407
                     /gene="yjjB"
                     /locus_tag="ACHY09_003025"
     CDS             934..1407
                     /gene="yjjB"
                     /locus_tag="ACHY09_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFS3885973.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1515..2054
                     /gene="dnaT"
                     /locus_tag="ACHY09_003026"
     CDS             1515..2054
                     /gene="dnaT"
                     /locus_tag="ACHY09_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFS3885974.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2057..2794
                     /gene="dnaC"
                     /locus_tag="ACHY09_003027"
     CDS             2057..2794
                     /gene="dnaC"
                     /locus_tag="ACHY09_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFS3885975.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2843..3337
                     /gene="yjjA"
                     /locus_tag="ACHY09_003028"
     CDS             2843..3337
                     /gene="yjjA"
                     /locus_tag="ACHY09_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFS3885976.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3591..5882
                     /gene="opgB"
                     /locus_tag="ACHY09_003029"
     CDS             3591..5882
                     /gene="opgB"
                     /locus_tag="ACHY09_003029"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFS3885977.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6020..7042)
                     /gene="lgoD"
                     /locus_tag="ACHY09_003030"
     CDS             complement(6020..7042)
                     /gene="lgoD"
                     /locus_tag="ACHY09_003030"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFS3885978.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGALVAVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7181..8095
                     /gene="lgoR"
                     /locus_tag="ACHY09_003031"
     CDS             7181..8095
                     /gene="lgoR"
                     /locus_tag="ACHY09_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFS3885979.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8310..9671
                     /gene="lgoT"
                     /locus_tag="ACHY09_003032"
     CDS             8310..9671
                     /gene="lgoT"
                     /locus_tag="ACHY09_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3885980.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMIGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9720..11384)
                     /gene="tsr"
                     /locus_tag="ACHY09_003033"
     CDS             complement(9720..11384)
                     /gene="tsr"
                     /locus_tag="ACHY09_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFS3885981.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAASEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            11566..12429
                     /locus_tag="ACHY09_003034"
     CDS             11566..12429
                     /locus_tag="ACHY09_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS3885982.1"
                     /translation="MAYSIDIISCITERFMALTATEKRIAQFILDDVSAAVELPIAEL
                     ARLTQTSQASVTRFARALGCKDVRELKVKLAQSLAVGQRFILDVPDLDGVQGIYEAII
                     SVLETHRRSLDMNAISQAVSWLSQARQILALGTGGGSTICSQEIQYRLFRLGLPVVSQ
                     NDALMMRMMCSAVMPQDVVIVLSLGGYTPEIIESAAIARQYGARVIAITPAQTPLAEQ
                     VDLVLPLLVQESDYIFKPTPSRYAMLAMVDVLATELAMANKAQAKDRLRRIKLVLDSH
                     RGGANRQPLGD"
     gene            complement(12439..13908)
                     /locus_tag="ACHY09_003035"
     CDS             complement(12439..13908)
                     /locus_tag="ACHY09_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFS3885983.1"
                     /translation="MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLF
                     LTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMT
                     MSEELSYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
                     LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSL
                     FAIDKMGVLPALSLALVIGVGVLATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSIL
                     PAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSATMSSASSDAIAAVA
                     IMMRDVYTMITGRMPPEDKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMS
                     GLFICSILGRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVA
                     GVLVTLVTPASQVSREEALAIITNERENQSVVITKPEEA"
     gene            complement(14011..14400)
                     /locus_tag="ACHY09_003036"
     CDS             complement(14011..14400)
                     /locus_tag="ACHY09_003036"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFS3885984.1"
                     /translation="MSIKRYGTEGGTGTGGQHLPFARAVEAGGWLYVSGQTPMKNGEV
                     VEGGIVEQSRLAIQNCVDIMTEAGYSLADVVHVKVILTDARYFQSFNKVFREFFGENP
                     PARICCVADLVVDCKVEVDVTCYNATR"
     gene            complement(14410..15852)
                     /locus_tag="ACHY09_003037"
     CDS             complement(14410..15852)
                     /locus_tag="ACHY09_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS3885985.1"
                     /translation="MQVDWLIKNVTVIDGSGGPEFRGDVAITGDRIVDIAPALNVTAQ
                     QVIDGEGRVLAPGFIDVHTHDDINVIRIPEYLPKISQGITTVIVGNCGISAASAKMKG
                     EVPDPMNLLGEAEHFIYPTVESYAQAVEAARPSLNVGTLIGHTALRNNHMDDLFRPAT
                     VDEIAAMRADLRLALSQGALGLSSGLAYATAFQATTEEVMALAEELAGEKGVYTTHLR
                     SEFEPILDALDEAFRIGRHGKVPVVVSHHKCAGAKNWGRTKETLAFFDQMRQHQEIGC
                     DVYPYSASSSTLDLKQVTDEFDIVITWSQTHPQQAGKTLAQIAIDWQMSMLEAAKLLM
                     PAGAIYYNMDERDVRRVLSYPVSMIGSDGLPNDPMPHPRLWGAFPRVLGHYCRDEGLF
                     PLTTAIHKMTGLSASRFRLPQRGLVKVGYFADLVLFDPQTIRDVASFSDPKRPADGIE
                     AVMVNGVMSYGPDKHITGRAGRFLRRQTSH"
BASE COUNT         3943 a         4256 c         3905 g         3877 t
ORIGIN      
        1 atttgtttgc gacgagagat aattcgcttt ttaacaccga gcttctcccg ataaccagga
       61 ttaatcagcg caattttgat cttaacctgc cgcaaaacaa ccgtgcttca tgatacaaat
      121 ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag ttatgcaaac
      181 tgagcaacag cgagccgtaa cacggctttg tatccagtgt ggattatttc ttttgcaaca
      241 tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg cgttgggaat
      301 ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg ctgacaacta ttaaagatgg
      361 gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc attaatatgc atgtggtgac
      421 tgaagtccag cacattgtga ttctggcgga gcaccatctg ctggattaca aaggcgtaga
      481 gaaacgattt agccaaattc agccattacg ttacccaaga tggctggtag ccttaatggt
      541 tggcctttct tgcgcctgtt tctgtaaact caataacggt ggctgggatg gtgccgtcat
      601 caccttcttt gccagtacgg ccgcgatgta tatccgccag ctgctggcac aacgtcatct
      661 tcatccacag atcaactttt gcctcaccgc tttcgccgcc accaccattt ccggtttgct
      721 tttgcaactt cccactttca gcaatacccc caccatcgcg atggccgcca gcgttctgct
      781 gctggtgccg ggctttccgt tgattaatgc cgtcgccgat atgtttaaag gccacatcaa
      841 taccggactg gcacgctggg cgatcgccag tctgctgaca ctggctacct gcgtcggcgt
      901 ggtgatggca ctgacgattt gggggctacg cggatgggtg tgatcgaatt tctgttagcg
      961 ttggcgcagg atatgatcct cgccgccatt cctgcggtcg gctttgcgat ggtgttcaac
     1021 gttcccgtgc gggcgttacg ctggtgtgcg ttgcttggct cgataggtca tggttcccga
     1081 atgatcttaa tgaccagcgg gctgaatatt gagtggtcaa cttttatggc ttctatgctg
     1141 gtcggcacca ttggtattca atggtcgcgc tggtatctgg cgcatccgaa agtgtttacc
     1201 gtggcggctg ttattcctat gttccctggc atatcggctt ataccgcaat gatttcggcg
     1261 gtaaaaatca gccagctagg ttacagcgaa ccgttgatga ttaccctgtt aaccaacttt
     1321 cttacagctt catcgattgt tggtgcgtta tccatcggtc tttccattcc tggattatgg
     1381 ttgtaccgca agcgccctcg cgtataaaat tgcctcactt aaagcacgcg gatgagagac
     1441 agcctcctct cctccgtgtg ttactataaa agttatctcc cttctcgttc atcgttccat
     1501 atttgagaaa cagtatgtct tccagagttt tgaccccgga cgtcgttggt attgacgccc
     1561 tggtacacga tcaccaaacc gttctggcaa aagctgaagg cggtgtggtt gccgtatttg
     1621 ctaacaatgc cccggcgttt tatgccgtta cgcctgcacg cctggctgaa ctgctggcgc
     1681 tggaagaaaa gctggcgcgt ccgggaagcg atgtcgctct ggacgatcaa ctctatcagg
     1741 aaccgcaagc cgctcccgtt gctgtaccca tggggaaatt cgccatgtat ccggactggc
     1801 aacccgatgc cgactttatc cgcctggcgg cgctatgggg cgtggcgcta agagagccgg
     1861 tgaccaccga agaactggcc tcattcattg cctactggca ggcggaaggt aaagtctttc
     1921 accatgtgca gtggcaacaa aaactggcgc gcagcctgca aatcggtcgt gccagcaacg
     1981 gcggactgcc gaaacgagat gtgaatacgg tcagcgaacc tgacagccaa attccaccag
     2041 gattcagagg gtaacgatga aaaacgttgg cgacctgatg caacgcctgc aaaaaatgat
     2101 gcctgcccat atcaaacctg cgttcaaaac gggtgaagaa ctactggcct ggcagaaaga
     2161 acaaggagcg atccgctccg ccgctctcga acgtgaaaat cgggcgatga aaatgcagcg
     2221 cacctttaac cgctccggta ttcgtccact gcatcagaac tgctcctttg agaactatcg
     2281 cgttgagtgt gaagggcaga tgaatgcgtt aagcaaagcg cgccagtatg tcgaagagtt
     2341 tgacggcaac atcgccagct tcatcttttc tggcaagccc ggaaccggca aaaaccatct
     2401 ggcggcggca atctgcaacg agctgctgct acgcggtaaa tccgtattga tcatcaccgt
     2461 ggccgatatt atgtcggcga tgaaagatac cttcaggaat agcggtacca gcgaagaaca
     2521 actgcttaac gatctgagca acgttgatct gctggtgatc gatgagatcg gcgtgcagac
     2581 cgaatcgaaa tacgaaaaag tgatcatcaa ccagatcgtc gatcgccgct cttcttccaa
     2641 acgcccaacc gggatgctga ccaacagtaa tatggaagag atgaccaagt tgctgggcga
     2701 acgcgtgatg gaccgtatgc gcctgggtaa cagtttgtgg gtgatcttca actgggatag
     2761 ctaccgtagc cgggtaacgg gtaaagagta ttaatccccg tttctggcgg tatactgcga
     2821 cacaattcca ccttgagtaa tgatgatgaa aactgttaaa catcttctgt gctgtgccat
     2881 tgccgccagc gcattaattt ccaccggggt gcatgctgcg tcctggaaag atgcgctctc
     2941 cagcgccgcc agcgaacttg gcaaccaaaa cagcacgaca caggaaggcg gttggtcgct
     3001 cgcgtcatta actaacttgc ttagcagcgg aaaccaggcc ttaagcgcag ataacatgaa
     3061 caatgccgca ggcattctgc aatactgcgc gaagcaaaag ctggcttcgg taaccgacgc
     3121 cgaaaacatc aagaatcagg tactggaaaa actgggcctg aacagtgaag agcaaaaaga
     3181 agacaccaac tatctggatg gcattcaggg tttgctgaaa accaaagatg gtcagcaact
     3241 caatctggat aatattggaa cgactccgct ggcagaaaag gtgaaaacca aagcctgcga
     3301 tctggtgtta aagcaggggc tgaacttcat ttcctgaacc gcgaaacgcc gcgttttagc
     3361 tgttacccgg ctgacgcggc tctctcctgc ccttttccct gctcctggac ggttttcccc
     3421 ctgttacacg ggaagcgacc gcgatcaaaa aagtctgttt ataaactaat tcctaacaac
     3481 atcgcatttt tttgacacta gaattgcagc aatatggcga cgccattaca ttgtgcttct
     3541 caaaaagctt aaacgttggc taaacggccc ggtttagtga ggatcgcccg ttgtcagaac
     3601 tactctcttt cgcccttttt ctcgcctctg tgctgattta cgcatggaaa gcgggacgta
     3661 acacctggtg gtttgcagcc acattaacgg tactggggct atttgtcgtt ttaaatatca
     3721 ccctgtttgc cagcgactat tttactggcg atggtattaa cgacgcggtt ctctatacct
     3781 taaccaacag cctgaccggt gctggtgtca gtaaatacat cctgcctggt atcggcattg
     3841 tgctggggct gacagcggtg ttcggtgcgc tgggctggat cctgcgccgt cgtcgtcatc
     3901 atccgcacca ttttggttac agcctgctgg cactcttact ggcgttgggt tcagtggatg
     3961 ccagcccggc atttcgtcag ataacggaac tggtgaaatc ccagtcacgc gacggcgacc
     4021 cggactttgc ggcttattat aaagagccgt cgaaaactat ccctgacccg aaactcaacc
     4081 tggtttatat ctacggcgaa agtctcgagc ggacctattt tgataacgag gctttcccgg
     4141 atctcacgcc tgaactgggc gcgttgaaaa atgaaggcct ggatttcagc cacacgcagc
     4201 agctgccagg aacggattac acgattgcgg gcatggtggc ttctcagtgc ggaataccgc
     4261 tgtttgcccc ctttgaaggc aacgcctccg cttctgtttc cagcttcttc ccgcagaaca
     4321 tctgtctggg cgatatcctg aaaaactcgg gttatcagaa ctatttcgtg cagggcgcga
     4381 atctgcgttt tgccggtaaa gatgtgttcc tgaagtcgca tggcttcgac cacttatatg
     4441 gctcagaaga gctgaaaagc gtggtggccg acccgcacta tcgcaacgac tggggattct
     4501 acgacgatac cgttctcgac gaagcgtgga aaaagtttga agagctttcc cgctcaggtc
     4561 agcgattctc actgtttacc ctgacagtcg atacccatca cccggatggt tttatctcgc
     4621 gtacctgtaa ccgcaaaaaa tatgattttg acggtaagcc gaatcagtca ttcagcgcgg
     4681 taagttgcag ccaggagaat atcgcgacgt ttatcaacaa aatcaaagcg tcaccgtggt
     4741 ttaaagatac cgtcatcgtc gtctcttctg accatttagc gatgaacaac acggcgtgga
     4801 aataccttaa taaacaggat cgcaataacc tgttttttgt catccgtggc gacaagccgc
     4861 agcaagagac gctggcggtg aagcgtaaca cgatggataa cggcgcgacg gtgctggaca
     4921 ttctcggtgg cgataactat ctcggacttg gtcgtagcag tttatccggg cagtcaatgt
     4981 cggaaatctt cctcaatatc aaagagaaaa cattggcgtg gaagccggat atcatccgcc
     5041 tgtggaaatt ccctaaagag atgaaagagt tcaccatcga ccagcagaaa aacatgattg
     5101 ccttctcggg tagccatttc cgtttgccgc tactgttgcg ggtttcagac aaacgcgtgg
     5161 aaccgctgcc ggaaagtgaa tactcagcac cgctgcgttt ccagctggcc gatttcgctc
     5221 cacgcgacaa tttcgtctgg gttgaccgtt gctacaagat ggcacaactt tgggctccgg
     5281 aactggcact ctccaccgac tggtgtgtct cgcaagggca gcttggcggt cagcaaattg
     5341 tccaacatgt tgacaaaaca acatggaagg gcaaaacggc atttaaagat acggtcatcg
     5401 acatggcgcg ttacaaaggc aatgtcgata cgctgaagat tgttgataac gatattcgct
     5461 acaaagccga cagtttcatc tttaacgtcg ccggtgcgcc agaagaggtg aaacagttta
     5521 gcgggatttc ccgtccggag tcgtggggcc gctggtccaa cgctcagctg ggcgacgaag
     5581 taaaaatcga gtacaagcat ccgctgccga agaaatttga cctggtgatt accgccaaag
     5641 catacggcaa taacgccagc cgtcctattc cggtacgcgt aggcaatgaa gaacaaactc
     5701 ttgtgctggg caatgaagtg accaccacca cgctgcattt cgataaccca accgatgccg
     5761 acacgctggt aattgtgccg ccggaacctg tctcaaccaa cgaagggaat atcctcggac
     5821 actcgccgcg taagctcggg atcggcatgg tggaaattaa agtggtagaa cgtgaagggt
     5881 aattcctgac tgatgtgtag gtcgcataag gtgcgaccta cacgccttct ccggcttaca
     5941 acacactatt aagaaacaaa aaaaacagcg cccaacactt ccccggacgc tgcatggcaa
     6001 ctttaataat ctcgccttat cagaaagtaa ttacaccctt aatcaactca cgattgttaa
     6061 tcacatcacg ctcgtaggtt tctgccagcg tggcgaacgg gtagcgatgg gttaacatca
     6121 tgtcggcagt gattttccct tccgccatca gtcgaccgac tttagcgaaa tcttccggcg
     6181 tggcgttgcg gctacccatc atcgtcgttt cttttttatg gaattctgga tcggagaact
     6241 gcaactcacc tttaaacaga ccgacaaata ccaccgtgcc gccgtgacga atcagattca
     6301 cggtgttatt catcgcatgt tgattacctg tcgcgtcgat cactttctgc gccagcgaac
     6361 caccaaactg cgcccgcagc tgcgcgtcaa aatcttcggc tgatgggtcc agcacaggca
     6421 actccagacg cgttgccaca tgttcacggc gcgccggact ggtatccgcc accgctacca
     6481 gtgcgccatc ggctttagcg attgccgccg cgcccagacc gattggcccc gccccgacca
     6541 ccagcacctg ctcgccggga gcaatggctg cgcgacgcac cgcatgagcg ctaatagcga
     6601 aaggttcaat caatgctgcc gcctgcgggt caataccgtc tgcgggcaaa atgttcgcca
     6661 ccggcacgct caaatactca ctaaaaccgc catcctgatg cacgccaatg actgaaattt
     6721 tttcacagca attggtacgc ccgcttttac acgccgggca ttgctgacag gcaacataag
     6781 ggatcacagc aacttgctga ccatttttaa gatcagcaat atttttaccc agcccaacaa
     6841 tctccccaca tatttcatgg cctaaaacgc gtggataact aaaaaatggt tgatttccac
     6901 cccaggcatg aatatcggta ccgcaaatcc cgacagactt aatttttatt aatgcttcat
     6961 tgtcacccgg aataggtatc tcgcgttgtt tccagactaa ttctttcggc tgctggcaaa
     7021 ttaaaacatt catcgtagac atgattcggc tctccatttt ttgttgcctt agatatcaat
     7081 gaaaatgaaa aatggcagcg gaacggaaaa tctgttttgt gaaaacacac gcataaaaat
     7141 gttttaaatc gggttttaat ggactcacaa ggaggaaaat atgagtcgtt cacaaaattt
     7201 acgccacaat gtgattaacc aggtcattga tgatatggcg cggggtcata tcccttcccc
     7261 acttccatcg caaagtgcgc tggcggaaat gtacaacatc agccgcacta cagtgcgtca
     7321 tattctcagc cacttacgcg aatgcggcgt cttaacgcag gttggaaacg actatgtgat
     7381 tgcgcgtaaa cctgaccatg atgacggttt cgcctgtact accgcttcaa tgagtgaaca
     7441 aaacaaagtg tttgagcagg cttttttcac gatgataaac cagcgccagt tacgcccagg
     7501 ggaaactttt tctgaactgc aactggctcg ggccgcgggt gtcagcccgg ttgtagtaag
     7561 agaatatctt ttaaaattcg gccgttataa tcttattcaa agcgaaaagc gcggccagtg
     7621 gagcatgaag caattcgatc aatcctacgc tgagcagctg tttgagctac gcgaaatgct
     7681 cgaaacacat tcacttcagc actttctcaa cctgcctgat catgatcccc gctggctgca
     7741 ggcgaaaacc atgctcgaac gtcatcggtt actgcgtgac aacatcggca atagctttcg
     7801 catgttctcg caacttgaca gagattttca ctccttactg ctttcagctg ccgacaatat
     7861 cttttttgat caatcgcttg agatcatctc agtcatcttt cattttcact atcaatggga
     7921 cgaaagcgat cttaaacagc gcaatatcat tgccgtcgat gagcatatga ccattctcag
     7981 tgctctgatt tgccgcagtg accttgatgc cacccttgcg ctccgtaacc atttgaattc
     8041 agctaaacaa tcaatgattc gatccattaa tgagaacacg cgttatgctc attaagtacg
     8101 gattaaaaac agcaaactat tagcgggtgc acggttttat caaaagataa ataccgggaa
     8161 attcccgctt acctatgctc acaatcagtc gactaaaaat gtttttgtga gcggtagtaa
     8221 agtcctaaaa ctttaacctg ccgttaatgt caaccttgcg taatgcacaa ttacgtaaac
     8281 aattaaaatt ataaatacag gagtccggcg tggaaaaaga aaatatcacc ctcgatccgc
     8341 gttcttcatt tactccatct tcgtcggcag atattcccgt gccaccagat ggattagttc
     8401 aacgcagtac ccgaattaaa cgcattcaaa ccaccgccat gttgttatta ttttttgcgg
     8461 cggtaatcaa ttatctcgac cgcagttcgc tgtcggttgc aaatttaacg attcgtgaag
     8521 aattgggatt aagtgctacc gaaatcggcg ctttgctctc cgtgttttca ctcgcttacg
     8581 ggattgcgca acttccttgc ggcccactat tggatcgtaa aggcccacgc ctgatgctgg
     8641 gactggggat gttcttctgg tcactgttcc aggccatgtc tggcatggtg cacagcttta
     8701 cgcagttcgt gttggtgcgt atcggtatgg ggattggtga agcgccgatg aacccatgcg
     8761 gtgtaaaagt cattaacgac tggttcaaca tcaaagagcg cggacgcccg atgggcttct
     8821 tcaacgcagc ttctactatt ggcgttgccg taagcccacc gattctggcg gcgatgatgc
     8881 tggtgatggg ctggcgtggg atgtttatta ccattggtgt gctggggatt tttctcgcca
     8941 ttggctggta tatgctctat cgcaaccgcg agcacgtaga actgactgcc gttgaacaag
     9001 cttacctcaa tgctggtagc gtcaatgccc gccgagatcc gctcagtttt gccgaatggc
     9061 gcagcctgtt ccgtaaccgc acaatgtggg gaatgatgat cggattcagt ggcatcaact
     9121 acactgcgtg gctgtatctg gcctggcttc ctggttacct gcaaacagcc tataacctgg
     9181 atttaaaaag cacagggttg atggcggcta tccctttcct gtttggggct gccgggatgc
     9241 tggtcaacgg ttacgttacc gactggctgg tcaaaggggg aatggctccg attaaaagcc
     9301 gtaagatctg cattattgcc gggatgttct gttctgccgc ctttacgctg gtcgtaccgc
     9361 aagcgacaac atccatgaca gcggttctgc tgattggcat ggcactgttt tgtattcact
     9421 ttgccggaac atcctgctgg ggcttgatcc acgtcgcagt tgcttctcgc atgacggcgt
     9481 cggtgggcag tatccagaac tttgccagct tcatctgcgc ctctttcgcg ccgatcatta
     9541 ctggttttat tgttgatacc acccactcat tccgtctggc actaatcatc tgcggttgcg
     9601 tcaccgcagc gggggcactg gcgtacatct tcctggttcg tcagccgatc aacgacccgc
     9661 gtaaagatta aactccctat gcccgataac attttgctta tcgggcattt ttatggcgat
     9721 taaaacgttt cccaattctc gccgctatct gccacggcca ttttacgcgg cgtagctggc
     9781 gtcacggttt ttactacagc cgatgtttca cgctgctgtt gctgctgttg ctgaatccgg
     9841 aacactgcca ctgcttcggt caggcgactg gcctgctctt ccagcgcggc ggcggcagcg
     9901 gcagattctt ccaccagcgc ggcgttttgt tgggttaccc ggtccatctc agcaaccgct
     9961 aagccaacct gatcgatacc acggctctgc tcatcagaag cagaagcaat ttcgcccata
    10021 atgtccgtca cgcgggtcac ggcactgaca atctccgcca ttgtttcccc ggcgctttcg
    10081 accagcgtag agccaacatc cactttcccc accgagtctt caatcaggct tttaatttca
    10141 cgagcagcct gagcgctacg ctgggccaga ttacgcactt ctcccgcaac caccgcaaaa
    10201 ccgcgccctt gctcacccgc gcgcgctgct tcaaccgccg cgttcaaagc cagaatattg
    10261 gtctggaagg caataccgtc aattacgctg ataatatcgg cgattttctg cgaactggtg
    10321 gagatatcgc gcatagtctg caccacgtta tccaccactt tgccgccgcg ttgcgccgtt
    10381 tcggaagcac ttaacgccag gtggctggcc tgacgcgcat tttcggcgtt ctgcttcacc
    10441 gttgcggtca gttgctccat gctggctgcc gtctcttcca gcgaagcggc ctgttgctcg
    10501 gtgcgcgaag agagatcgtt attgccggtg gcgatttcgc tggcaccgct atagatggca
    10561 ttggccccgt tgcgcacatc accgacggta cgcatcagct ctccctgcat atggcgcaaa
    10621 ctctctgcca gttgccccat ctcattagag ccatccacct caatcggttt caccagatcg
    10681 ccgcctgcaa tatgacgaat gctgtcaatc aggcgattca ttggcgcgac cagcgaggct
    10741 ttaataccga accagacggc gaggatgacc gccagtacga cgatcatcac gcctaccaga
    10801 atccacatcg cctggctgta ggaggcattg ttatcgctga cggcgatatc atagagccga
    10861 tcgttttgct ccatgtaagc cacatactgc ttctcgaaac cgtcctgata tccctgggtc
    10921 ggttgatcaa agaactcgtt gattttgcct gcacctaaca gttggatcag ctccgccagc
    10981 gcattgtgat aaatatcgta attacgtttg atctctgacg ctgcggcggt gctctgacgc
    11041 gggtcacgcg gcaacgcttc gtaatccgcc cagttttttt ccgcctgttt cagcgaaata
    11101 ctggcactct gcatcagctc agcaacggtt gaaccgctac caatattatt ctgatccatc
    11161 atgtagcgga tacccgcgcg gttgagggtg ttacgcgtct gcaacaacgc aacccagctg
    11221 ccattcagcg tggattgctg ctggcgaatg gtttgtaaaa cagtgaaatt ttctttgtca
    11281 ttctttaagg cattaaagaa cagaccgcct gatgtcagtt gtaaaaggcc aaaaacggcc
    11341 aaaaccagca gtaagctggt cacgatcttg atacgtttta acatggtttc tctttccact
    11401 agacagatac actggttgtc ggcctggtgg tggaaaactt tatgctgtgg aaaagcgtcg
    11461 gcactgcttt tgttgcagca aacttgcgtt ttttgtgagg gtcacactct atagcggcag
    11521 tttatggtac aaagtatcaa tcttcttcga accagtgata ctcctatggc ttatagcatc
    11581 gatatcatct cctgcattac cgaacggttt atggcgttaa cagccactga aaagcgtatc
    11641 gcacaattta ttcttgatga cgtctctgcc gcagtagagt tgccaattgc tgagctggca
    11701 cggttaacgc aaaccagtca ggcgtcggtg acgcgttttg cccgtgcact ggggtgcaaa
    11761 gatgtccgcg agttgaaagt caaactggcg cagtcactgg ctgtcgggca gcggtttatt
    11821 ctcgatgtac cggatcttga cggtgttcag gggatctacg aagcgattat tagcgtgctg
    11881 gagacgcatc gtcgttcgct ggatatgaat gccatcagcc aggctgtaag ctggttaagt
    11941 caggcgcggc aaattcttgc gttaggaact ggcggcggtt ccactatctg ttcacaggag
    12001 attcagtacc gtttattccg tctggggcta ccggtggtca gtcagaatga tgccctgatg
    12061 atgagaatga tgtgctcggc agtcatgccg caggatgtgg tgattgtgct ttcgctgggc
    12121 ggttatacac cggaaatcat cgaaagtgcg gcaatcgccc gtcagtatgg cgcgcgtgtt
    12181 attgccatta cgccagcaca aacaccgctg gcggaacagg tggatctggt attgccgtta
    12241 ctggttcagg aaagtgatta catctttaag cccacccctt cccgctacgc gatgttagcc
    12301 atggtcgatg tactggcaac agaactggca atggcaaata aagcgcaggc gaaagatcgc
    12361 ctgcggcgga taaaactcgt gttagacagc caccgtgggg gcgctaaccg ccagccactt
    12421 ggcgattagc attgtcggtt atgcttcttc aggtttggtg atcacgacac tctggttttc
    12481 acgctcattg gtgataatag ccaaagcttc ttcccggctg acctgacttg ctggcgttac
    12541 caacgtcacc agtacgccag cgaccaggct gcccagcact gacggaatac acgggttacc
    12601 ccagtaagcc agccagtcgg cattcatcag caccacaatt gacatcacca taccgctcac
    12661 taacgccgcc agcgcgccct gccagttaaa gcgtagccag aagcgaccga gaatcgagca
    12721 gataaacagc ccggacatca gcatcgaaat cattttggta atgtagctga tgatgtcatt
    12781 agaggtcaga gcaaagacca gcgccaggcc aataacaaag gtcaacatcc agcgtgagta
    12841 agtaatggcc ttgtcctccg gcggcattct gccggtaatc atggtgtaga cgtcgcgcat
    12901 cataatcgcc accgccgcga tggcatcgga gctggcagaa gacatggtgg ctgaaagccc
    12961 ggcaatcagc accaccagcc caagaatggc aggtaagaaa ctggtcgcga acaagaaggc
    13021 gtagttgctg ttttccagat ttgggttcat ggtgaacgcc gccatgccga taatggcagg
    13081 caaaatcgag aagaacagat ataacacccc agtaataaca aacgaacggc gtacagaggg
    13141 aacatcttta cccgagtaaa tacgctggcg ataagacggt gttgccagca cacctacgcc
    13201 aataaccagt gccagcgaaa gcgcaggcag cacgcccatt ttatcaatcg caaacaggct
    13261 catcgctttt gggtccatcg cctgctcaat cgcactccag ccgccaacgt gaacaacggc
    13321 aagaatagcc attaatataa agccaaagaa cagaattagg gcctgaatag tatctgtcca
    13381 cactaccgcc gaataaccgc cgataataac ataaatcgca aaagctaagg cgataattaa
    13441 ttttgccaca gtaagatcaa tacccgtcgc ccaggcaaga tacatacttc cacctaatat
    13501 atgcgccccc agccagccaa tagaggcaat aaatatcatc aggccgacaa tatttttaat
    13561 taaatgacta ccgccagtat aataagagag ttcttcactc atggtcataa aacgtaattt
    13621 acgtaccggt gcaaacagcc acgccaccag caatatacct acggctccac cgacgccata
    13681 aagcattccg gcccagccat tgctgtagcc aaagcccact gcccccatac tggaacccgt
    13741 gccaaccatc gtggcaacgg ttgaccccag cgtcagaaac agcggcagcg aacgcccgcc
    13801 cagcagaaaa tcttctccgc ttttctgatt acgagaaaca aaccaaccaa gccaaatcat
    13861 ggcgatggca taaataataa agccgactaa aaagatatga ctgttcataa ttcacatcct
    13921 gccagtagag agaaattaat gtaagagttc ccggagtgct gaatgtattc agcactcaat
    13981 tgcggtctgt ttttttaatt cttaaattta ttagcgggtc gcgttataac aggtcacatc
    14041 gacttcgact ttacaatcga caaccagatc agccacacaa caaatacgtg caggcggatt
    14101 ctcgccaaaa aattctctga atactttatt aaatgactgg aaatagcggg cgtcggtcag
    14161 gatcactttc acgtgaacca catccgccag gctataaccg gcttcagtca tgatgtcgac
    14221 acagttttgg atcgccagtc gtgactgttc aacgattccg ccttctacca cttcaccgtt
    14281 tttcatgggt gtttgcccgg agacgtacag ccagccgccc gcctcaaccg ccctggcaaa
    14341 aggcaaatgc tgcccgccag tgcctgttcc accttccgta ccgtaacgtt taatgctcat
    14401 attcactcct taatgtgagg tctgccggcg caggaagcgt cccgcacggc ctgtaatatg
    14461 tttatcggga ccatagctca taacgccgtt caccatcaca gcctcaatgc catctgccgg
    14521 gcgtttggga tcagaaaagc tggcaacatc acgaatggtt tgtggatcga acaacaccag
    14581 gtccgcaaaa taaccgactt tcaccagtcc acgctggggc aggcgaaaac ggctggcaga
    14641 aagcccggtc attttgtgga tagccgtggt cagtgggaat aagccttcat cacggcaata
    14701 gtggcccagc acgcgaggga aggcgcccca taaacgtgga tgcggcatgg gatcattggg
    14761 caggccgtca gagccaatca tactgaccgg ataactcaac actcggcgga cgtcgcgctc
    14821 gtccatgttg taatagatag ccccggcagg cattagcagc ttcgcggcct ccagcatact
    14881 catctgccag tcgatggcga tttgcgccag tgttttcccg gcctgctgcg gatgtgtttg
    14941 tgaccaggtg atcacaatat cgaattcgtc ggtgacttgt ttgagatcca gcgttgaaga
    15001 gctggctgag taagggtaga catcgcagcc aatttcctgg tgttgacgca tttggtcaaa
    15061 aaaggccaac gtttcttttg tccgccccca gtttttcgcc ccggcgcatt tatggtggga
    15121 aaccacgaca ggcactttgc catgacgccc aatacgaaac gcctcatcca gggcatcgag
    15181 aatcggttca aattcagaac gcagatgggt ggtataaacg cccttctcac cggctaattc
    15241 ttccgccagc gccatgactt cttcggtagt cgcctgaaat gccgttgcat aggccagccc
    15301 ggaacttaac cccagcgccc cctgactcaa cgccagacgt aagtcggcac gcatagcggc
    15361 aatttcatcc accgtcgccg ggcgaaacag gtcgtccata tggttattac gcagcgccgt
    15421 atgaccaatc agcgtgccaa cgttcagtga cggcctggct gcttccactg cctgggcata
    15481 gctttcaacg gtaggataaa taaagtgctc tgcttccccc aacagattca tcgggtcagg
    15541 aacttcacct ttcattttcg ccgacgccgc actaatcccg cagttaccga caatcaccgt
    15601 agtgatcccc tggctgatct ttggcagata ttccgggatg cgaataacgt tgatatcgtc
    15661 atgggtatga acgtcgataa acccgggtgc cagcacccgc ccttccccgt caatgacctg
    15721 ctgcgccgta acgttaagcg caggggcaat atcgacaatc cgatcacctg ttattgccac
    15781 gtcaccgcga aattcagggc cgccgctacc gtcgataacg gtcacatttt tgattaacca
    15841 gtcaacctgc atgttcgtca ctccatttct gcaatggtga caagttaacc atttacaagg
    15901 acgaaaaaca gtggaaaact acgcaaaacc cagatgattt tatgatactt ttattcataa
    15961 ataataagat aaataaacta a
//
LOCUS       DAZUCC010000114        15961 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZUCC010000114 DAZUCC010000000
VERSION     DAZUCC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.114"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..97
                     /locus_tag="ACHY09_003038"
     CDS             <1..97
                     /locus_tag="ACHY09_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HFS3885986.1"
                     /translation="NEIFRMAAIAFILLTVLVWFAKPPFTAKGVG"
     gene            complement(202..1533)
                     /gene="dsdA"
                     /locus_tag="ACHY09_003039"
     CDS             complement(202..1533)
                     /gene="dsdA"
                     /locus_tag="ACHY09_003039"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFS3885987.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLTQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLHNATHLVWATGGGMVPEEEMEQYLAKGRS"
     gene            complement(1551..1769)
                     /locus_tag="ACHY09_003040"
                     /pseudo
     CDS             complement(1551..1769)
                     /locus_tag="ACHY09_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2012..3007
                     /locus_tag="ACHY09_003041"
     CDS             2012..3007
                     /locus_tag="ACHY09_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS3885988.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHISLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEDREGGRVTRIPCPLLIRCST"
     gene            complement(3015..4448)
                     /gene="cscA"
                     /locus_tag="ACHY09_003042"
     CDS             complement(3015..4448)
                     /gene="cscA"
                     /locus_tag="ACHY09_003042"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS3885989.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDSGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            4664..5578
                     /locus_tag="ACHY09_003043"
     CDS             4664..5578
                     /locus_tag="ACHY09_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFS3885990.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDWDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5650..6897
                     /locus_tag="ACHY09_003044"
     CDS             5650..6897
                     /locus_tag="ACHY09_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3885991.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(6977..7159)
                     /locus_tag="ACHY09_003045"
     CDS             complement(6977..7159)
                     /locus_tag="ACHY09_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS3885992.1"
                     /translation="MWRDSILHSAMHSLMNSQARDTRDTSLNDDNVLEISEPRIRSTF
                     ERLYQNMNEAFGLGDY"
     gene            complement(7339..7413)
                     /locus_tag="ACHY09_003046"
     tRNA            complement(7339..7413)
                     /locus_tag="ACHY09_003046"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7378..7380),aa:Arg,seq:cct)
     gene            complement(7489..8421)
                     /gene="yfdC"
                     /locus_tag="ACHY09_003047"
     CDS             complement(7489..8421)
                     /gene="yfdC"
                     /locus_tag="ACHY09_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS3885993.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKTIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            8715..9470
                     /gene="mlaA"
                     /locus_tag="ACHY09_003048"
     CDS             8715..9470
                     /gene="mlaA"
                     /locus_tag="ACHY09_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFS3885994.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(9532..10872)
                     /gene="fadL"
                     /locus_tag="ACHY09_003049"
     CDS             complement(9532..10872)
                     /gene="fadL"
                     /locus_tag="ACHY09_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFS3885995.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIRQSPAWLTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNPAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            11244..11528
                     /gene="yfcZ"
                     /locus_tag="ACHY09_003050"
     CDS             11244..11528
                     /gene="yfcZ"
                     /locus_tag="ACHY09_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFS3885996.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            11709..13019
                     /gene="fadI"
                     /locus_tag="ACHY09_003051"
     CDS             11709..13019
                     /gene="fadI"
                     /locus_tag="ACHY09_003051"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFS3885997.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLLDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEMDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            13019..15163
                     /gene="fadJ"
                     /locus_tag="ACHY09_003052"
     CDS             13019..15163
                     /gene="fadJ"
                     /locus_tag="ACHY09_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFS3885998.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSGGYDAEARAFGEL
                     VMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDTALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            15366..15851
                     /gene="sixA"
                     /locus_tag="ACHY09_003053"
     CDS             15366..15851
                     /gene="sixA"
                     /locus_tag="ACHY09_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFS3885999.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         3822 a         3821 c         4125 g         4193 t
ORIGIN      
        1 aaatgagatt ttccgtatgg cggctattgc ttttatctta cttacggtat tggtttggtt
       61 tgcgaaaccg ccgtttacag cgaaaggcgt tgggtgatgg tataaagaga gaattatagc
      121 aggtaagacc ggggatctta agtctgcgcc atcggcagca tcgctcaccc agggaaagga
      181 tggcgatgct gcgttgaaac gttatgaacg gccttttgcc agatattgct ccatctcttc
      241 ttccggcacc attccacctc ccgtcgccca caccagatga gtggcattat gcagttgttc
      301 tgcgctgaaa ccgtgcatct gttggtaact tactgatgca cacacgcgct gaggtccggc
      361 catacccgcc agtgccgaag gttcaagacg aataccttct tcctgcgtca gccagccaag
      421 catgtcatac atggtttgat cgctaagggt atagaagcca tccagtaaac gctccattgc
      481 ccgcccgaca aagcctgatg cgcgaccaac tgcaaggcca tccgctgcgg taaggttatc
      541 gataccaata tcctgaacag aaatctgatc gtgtaatcct gtatggacgc ctaacagcat
      601 acaaggggag tgcgttggtt cggcaaaaaa gcagtgaaca tgatcgccaa atgccagttt
      661 aagcccgaat gcgacgccac caggaccacc gccaacacca cacggcagat agacaaacag
      721 agggttatca gcatcgacaa tacgaccttg ctcggcaaat tgcgctttaa gacgctggcc
      781 agcgacggaa tacccaagga acaacgtgcg ggaattctcg tcatcaataa agaaacagtt
      841 cgggtcagat tgcgctgctt tacgtccttc ctcgactgca acaccataat cttgctcata
      901 ttccacgacc gtaacgccat ggctgcgcag tttcgctttt ttccatgccc gggcatcagc
      961 agacatatgt actgtcacct taaagccaat gcgggcgctc ataatgccga ttgataaccc
     1021 cagatttccg gttgagccca cagcaatgct gtattgacta aagaactgtt taaactccgg
     1081 agaaagcagt ttgctgtagt catcttcaag cgtcagcaac cccgcttcca gagccagttt
     1141 ttctgcatgt gccaggactt cataaatccc gccgcgtgct tttatggagc cggaaatggg
     1201 caaatggcta tcttttttca gtagcagttg cccgctgatc ggttgttgat attccttttc
     1261 cagccgtttt tgcatagccg gaatggcaac cagttctgat tcaataatcc ccccagtagc
     1321 agcagtttca ggaaatgctt ttgccagata gggtgcaaaa cgggataagc gcgcatgggc
     1381 gtcctgaaca tcctgttcgg tcaggccaac ataaggtaaa ccttcagcca atgaggtcgt
     1441 gccaggatta aaccaggtgg tttctttaag agcaaccaga tcctttacca acggatactg
     1501 ggcgatgagc gagttcattt tagcgttttc catagtacgt ctctttgcgc ttagatgata
     1561 aatgacagca ggaatgtgcc cgccagagcg acgactgaag cgataaatgt cgctgtcgta
     1621 tagtatttaa atgtttcatt gagcgtagcg ccgcaatatt gcttcaccag ccagaaaagc
     1681 gagtccgtaa cgatcgtgca gccaatcgca cctgaaccga tagcaatcgc aataatttcc
     1741 ggactgatgt cgggatacag cggcagcaca atgatccata tttatatttt cttctccagg
     1801 ttggcgatgc cagttggcat gttttaccta cttgcctaca tggttactcg ttggcgagaa
     1861 cagaggcgga attgggtaca attgtccggg aattattgtt attttttatt gtattgccgt
     1921 gtcgggtgat agggcgttga gaatatcgtt ggatctcgcc agtgacgtct gtttctgcta
     1981 cagtgcccgt tttacggcaa acggcttggg tatggcttca ttaaaggatg tcgcacgcct
     2041 ggcgggagtg tcgatgatga cagtctcccg ggtgatgcat aatgcagaat ctgtgcgtcc
     2101 tgcaacgcgt gaccgcgtat tgcaggcaat ccagaccctg aattatgttc ctgatctttc
     2161 cgcccgtaag atgcgcgctc aaggacgtaa gccgtcgact ctcgccgtgc tggcgcagga
     2221 cacggccacc actcctttct ctgttgatat tctgcttgcc attgagcaaa ccgccagcga
     2281 gtttggctgg aatagttttt taatcaatat tttttctgaa gatgacgctg cccgcgcggc
     2341 acgtcagctg cttgcccacc gtccggatgg cattatctat actacaatgg ggctgcgaca
     2401 tatctcgctg cctgagtctc tgtatggtga aaatattgta ttggcgaact gtgtggcgga
     2461 tgacccagcg ttacccagtt atatccctga tgattacact gcacaatatg aatcaacaca
     2521 gcatttgctc gcggcgggct atcgtcaacc gttatgcttc tggctaccgg aaagtgcgtt
     2581 ggcaacaggg tatcgtcggc agggatttga gcaggcctgg cgtgatgctg gacgagatct
     2641 ggctgaggtg aaacaatttc acatggcaac aggtgatgat cactacaccg atctcgcaag
     2701 tttactcaat gaccacttca aatcgggcaa accagatttt gatgttctga tatgtggtaa
     2761 cgatcgcgca gcctttgtcg cttatcaggt tctcctggcg aagggggtac gtatcccgca
     2821 ggatgtcgcc gtaatgggct ttgataatct ggttggcgtc gggcatctgt ttttaccgcc
     2881 gctgaccaca attcagcttc cacatgacat tatcgggcgg gaagctgcat tgcatattat
     2941 tgaagatcgt gaagggggaa gagtgacgcg gatcccttgc ccgctgttga tccgttgttc
     3001 cacctgatat tatgttaacc cagttgccag agtgctccat gttgcagcac agccactccg
     3061 tgggaggcat agagcgacag ttcccgttct tctggctgcg gatatattcg gctactcatc
     3121 accgcctccc cgtcgttaat aaatacttcc acggatgatg tatcgataaa tatccttagg
     3181 gcgagcatgt caccctgcgg gaggggaata ctacggtagc catctaaatt ctcgtgtggg
     3241 taataccgcc acaaaacaag tcgctcagat tggttatcaa tatacagccg cattccagtg
     3301 ccgagctgta atccgtaatg ttcggcatca ctgttcttca acgcccactg cagctgaatc
     3361 tcaactgctt gcgcgttttc ctgcaaaaca tatttattgc tgattgtgcg gggagagata
     3421 gattgatgct gctggcgtaa cgactcagct tcgtgtaccg ggcgttgtag aagtttgcca
     3481 ttgctctctg atagctcgcg cgccagcgtc atgcagcctg cccagccttc acgttttgag
     3541 ggcattggcg attcccacat atccatccag ccgataacaa tacgccgacc atccttcgct
     3601 aaaaagcttt gtggtgcata aaagtcatgc ccgctatcaa gttcagtaaa atgcccggat
     3661 tgtgcaaaaa gtcgtcctgg cgaccacatt ccgggtatta cgccactttg aaagcgattt
     3721 cggtaactgt atccctcggc attcattccc tgcggggaaa acatcagata atgctgatcg
     3781 ccaaggctga aaaagtccgg acattcccac atatagcttt cacccgcatc agcgtgggcc
     3841 agtacgcgat cgaaagtcca ttcacgcaat gaactgccgc gataaagcag gatctgcccc
     3901 gtgttgcctg gatctttcgc cccgactacc atccaccatg tgtcggcttc acgccacact
     3961 ttaggatcgc ggaagtgcat gatgccttct ggtggagtga ggatcacacc ctgtttctcg
     4021 aaatgaatac catcccgact ggtagccaga cattgtactt cgcgaattgc atcgtcatta
     4081 cctgcaccat cgagccagac gtgtccggtg tagataagtg agaggacacc attgtcatcg
     4141 acagcactac ctgaaaaaca cccgtctttg tcattctcgt ctcctggcgc tagcgcaata
     4201 ggctcatgct gccagtggat catatcgtcg ctggtggcat gtccccagtg cattggcccc
     4261 cagtgttcgc tcatcgggtg atgttgataa aacgcgtgat aacgatcgtt aaaccagatt
     4321 aggccgtttg gatcgttcat ccacccggcg ggaggcgcga ggtgaaaatg gggatagaaa
     4381 gtgttacctc ggcgctcgtg aagttttgct agtgcgtttt gcgccgcatg taatcgagat
     4441 tgcgtcattt ttatcatcct ggttaagcaa attgggtgaa ttgttaacgt taacttttat
     4501 aaaaataaag tcccttactt tcataaatgc gatgaatatc acaaatgtta acgttaacta
     4561 tgacgttttg tgatcgaata tgcatgtttt agtaaatcca tgacgatttt gcgaaaaaga
     4621 ggtttatcac tatgcgtaag tcagatgaat ttaagggaaa aaaatgtcag ccaaagtatg
     4681 ggttttaggg gatgcggtcg tagatctctt gccagaatca gacgggcggc tactaccttg
     4741 tcctggcggc gcgccagcta acgttgcggt gggaatcgcc agattaggcg gaacaagtgg
     4801 gtttataggt cgggtcggtg atgatccttt tggtgcgtta atgcaaagaa cgctgctaac
     4861 tgagggtgtc gatatcacgt atctgaagca agatgaatgg caccggacat ccacggtgct
     4921 tgtcgatctg aacgatcaag gagaacgttc atttacgttt atggtccgcc ccagtgccga
     4981 tcttttttta gagacgacag acttgccctg ctggcgacat ggcgaatggt tacatctctg
     5041 ttcaattgcg ttgtctgccg agccttcgcg taccagcgca tttactgcga tgacggcgat
     5101 ccggcatgcc ggaggttttg tcagcttcga tcccaatatt cgtgaagatc tatggcaaga
     5161 cgagcatttg ctccgcttgt gtttgcggca ggcgctacaa ctggcggatg tcgtcaagct
     5221 ctcggaagaa gaatggcgac ttatcagtgg aaaaacacag aacgattggg atatatgcgc
     5281 cctggcaaaa gagtatgaga tcgccatgct gttggtgact aaaggtgcag aaggggtggt
     5341 ggtctgttat cgaggacaag ttcaccattt tgctggaatg tctgtgaatt gtgtcgatag
     5401 cacgggggcg ggagatgcgt tcgttgccgg gttactcaca ggtctgtcct ctacgggatt
     5461 atctacagat gaaagagaaa tgcgacgaat tatcgatctc gctcaacgtt gcggagcgct
     5521 tgcagtaacg gcgaaagggg cgatgacagc gctgccatgt cgacaagaac tggaatagtg
     5581 agaagtaaac ggcgaggccg ctcttatctc taaataggac atgaattttt taacgacagg
     5641 caggtaatta tggcactgaa tattccattc agaaatgcgt actatcgttt tgcatccagt
     5701 tactcatttc tcttttttat ttcctggtcg ctgtggtggt cgttatacgc tatttggctg
     5761 aaaggacatc taggattaac agggacggaa ttaggtacac tttattcggt caaccagttt
     5821 accagcattc tatttatgat gttctacggc atcgttcagg ataaactcgg tctgaagaaa
     5881 ccgctcatct ggtgtatgag tttcattctg gtcttgaccg gaccgtttat gatttacgtt
     5941 tatgaaccgt tactgcaaag caatttttct gtaggtctaa ttctgggggc gctctttttt
     6001 ggcctggggt atctggcggg atgcggtttg cttgacagct tcaccgaaaa aatggcgcga
     6061 aattttcatt tcgaatatgg aacagcgcgc gcctggggat cttttggcta tgctattggc
     6121 gcgttctttg ctggtatatt ttttagtatc agtccccata tcaacttctg gttggtctcg
     6181 ctatttggcg ctgtatttat gatgatcaac atgcgtttta aagataagga tcaccagtgc
     6241 atagcggcgg atgcgggagg ggtaaaaaaa gaggatttta tcgcagtttt caaggatcga
     6301 aacttctggg tttttgtcat atttattgtg gggacgtggt ctttctataa catttttgat
     6361 caacaactct ttcctgtctt ttatgcaggt ttattcgaat cacacgatgt aggaacgcgc
     6421 ctgtatggtt atctcaactc attccaggtg gtactcgaag cgctgtgcat ggcgattatt
     6481 cctttctttg tgaatcgggt agggccaaaa aatgcattac ttatcggtgt tgtgattatg
     6541 gcgttgcgta tcctttcctg cgcgctgttc gttaacccct ggattatttc attagtgaag
     6601 ctgttacatg ccattgaggt tccactttgt gtcatatccg tcttcaaata cagcgtggca
     6661 aactttgata agcgcctgtc gtcgacgatc tttctgattg gttttcaaat tgccagttcg
     6721 cttgggattg tgctgctttc aacgccgact gggatactct ttgaccacgc aggctaccag
     6781 acagttttct tcgcaatttc gggtattgtc tgcctgatgt tgctatttgg cattttcttc
     6841 ctgagtaaaa aacgcgagca aatagttatg gaaacgcctg taccttcagc aatatagacg
     6901 taaacttttt ccggttgttg tcgatatctc catatccctc aaccggaaaa taataataca
     6961 aaaatgctta cccctgctaa taatcaccta atccaaacgc ctcattcatg ttctggtaca
     7021 gtcgctcaaa tgtactccgg atacgcggtt cgctgatttc caggacattg tcgtcattca
     7081 gcgacgtgtc ccgtgtatca cgggcctgcg aattcatcaa ggaatgcatt gcagagtgaa
     7141 gtatcgagtc acgccatatt tcgctatcag gattctgtat gatggttaca tcgcccggcc
     7201 cagggctgtt tagtcatcag cgctttctga cagtgctgag atttcaacct gttgcagtaa
     7261 aaatgagtag atatagggca agtgtgctgc caaacccatc ttttacgggg tgaaggtaga
     7321 tttggtttga agggtatctg gtgtcccctg caggaatcga acctgcaatt agcccttagg
     7381 aggggctcgt tatatccatt taactaagag gacaatgcgg catgagtata cccgctaatg
     7441 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgccgggtt
     7501 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttgcg
     7561 cttgttgctc atgtcgttac ggatctgtgc atgactcatt aacgcgaaga taaaggtgcc
     7621 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
     7681 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgacaacga tgtgagtggt
     7741 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacccgc
     7801 tgcaggaaac atccaaacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     7861 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     7921 ttcttcattg aagattggca tatattcaaa tgcccacgcc gcaatacctg tcccgagaat
     7981 attacccagc agcacgacgc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     8041 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     8101 cataatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     8161 cacgccttcc agttcgacat gaaatatccc ttttgccagt agcgaagcgc ccatcgacag
     8221 acccgccgca atggctgacc acagtagtgc cattgcgtcg cgttccagct ctttttcacc
     8281 atcctggcgg atatgctcat gaattgccat cgcccgggag gggagtcgat cttcatctat
     8341 ttctattgtt ttgccgcgct ctttttcttc gctctcaact tcaatttcgt cgctgtgttg
     8401 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     8461 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     8521 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     8581 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     8641 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     8701 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     8761 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgcta gaagggttca
     8821 accgcaccat gtacaacttc aatttcaatg tattagaccc gtatattgtt cgaccggtcg
     8881 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa cggtttgagc aactttactg
     8941 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     9001 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     9061 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     9121 cgcttggtca ttatggtgtg ggttatgggc catacgttca gttaccgttc tacggtagct
     9181 tcacgctgcg tgatgacggt ggtgatatgg cggatggtct ttacccggtt ctttcctggc
     9241 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatagaaact cgtgcgcagt
     9301 tgttggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     9361 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     9421 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaaca
     9481 aaaaaggtga gtcgcaaaac tcaccttttt tcttattaag cgaacgcgtt atcagaacgc
     9541 gtagttaaag ttagtaccga acagccaggc tttaccttca gactcgaact ggtatgggcc
     9601 ttcgttaatt ttcacgctct gaccgtgcat ataagaaaca ccaacatcga ctgaagcatc
     9661 tttattaaat gcgtaagtcg tacctgcact cagccagaaa cggtcctggt ccggaatgga
     9721 gatagaacga ttctgtgccg gaactgggct gtcatcaaag gcgataccgg tacggaaggt
     9781 ccagttatca tcgtagtaat aagtggttcc caacgcgatg cggtaagcat ctttaaagcc
     9841 ttcatgtttc tggaacagcg tgtcgccact cgttgaggtc gctttcagct gctggaactg
     9901 actccagctg gtgtaagcca ggctatagtg aattgcccac tgcggatcaa cacggttata
     9961 acccgacact tcccacattt caggcaggtt cagcgtcaga taacccgact gcgttgcgcc
    10021 acctgtcgcg gtaggaattg gcaaaccgta gttattaaaa gccgggttaa gatcgctgct
    10081 gtagttaccc ttgaagtcaa ttttcacttc agagcggtag gtcaatgcgt agcggttgtt
    10141 tttatccagt tcatacagga taccggcgtt ccagccaaag ccccactggt taccgttcag
    10201 gtgagcgatt ttggtattac tgtcgatgcc gttggcggta gctgctaatg cttgcccctg
    10261 aggagtcagc caagcaggag attgcctaat ctgaccagcc accagttgcc ccagatcgcc
    10321 tgcgaaacgt tcaattttcg cgcgagcgta gacggcgttg aaaccaagac caaagctcca
    10381 tgcattattt aagcgatatg caccgcttaa gttcaggttc atggtttcga ggtcggtggt
    10441 accaccgaca gaaccgcctg cataagtatc gttaaactct gtcgccagac catagttaga
    10501 ggtaattgaa gccccccaac caaattggtc gttaatcggt gcaacaaagt gcatgttcgg
    10561 aacccatgcc gtaggcgcga tgttatcggc tttcaggcta cgaccagacg gagacgttcc
    10621 gctgatattt acatccggat caatataaac cgcacccgca gaaaatgtcg ggcggtcaaa
    10681 catggtaatc aatgcggggt tacggctaac gttacctgca tcatcggcaa ttgcaccttc
    10741 ccctgaataa gcccggccca ggccagagga agaaaattcg tttaactgaa agcctgccga
    10801 ccaggcctgg gtggagataa gtgccactgc gactgcgaga gcagactttg taaacagggt
    10861 tttctggctc atgaccataa cctcaatgat ttatttttat acaaactatg ttacgtgctg
    10921 taacaggagc gcgaagtgta gggtctgagg tacatcttag aaatcagacc agtggcgaga
    10981 gtataggtcg gaccagctgg aatgttgcaa gtatgtttct atcttttttc agatatgatc
    11041 taagaaacgc gatccagctg gcaaaattgg gaggtggcgc actcatggat tcgggtgaat
    11101 tttgttttag atcattttta agtgtgattt cggtcactta tccgattttg cagaattaac
    11161 aactggagca gcactttccg gaagcgtaaa atatccacaa cgtttatctg gcaccttggg
    11221 tgcacataca gaggaaatca acgatgagta aatgtagtgc tgatgaaacc ccggtttgct
    11281 gctgtatgga tgttggcacc attatggaca actccgactg caccgcgtct tacagccgtg
    11341 tgtttgcaaa ccgcgcagaa gcagaacaaa cgctggcggc gctgactgaa aaagcccgta
    11401 gcgtggaatc cgaaccgtgc aaaatcaccc cgactttcac tgaagagtct gacggtgtgc
    11461 gcctggatat cgatttcact tttgcctgcg aagctgaaat gctgatcttc cagctgggtc
    11521 tgcgttaatt cctctcgggt acatacgccc ctgtcagttg ctggcagggg cgttttcttt
    11581 tctcttctac ttttctctct gtgtcatagc tcccactttc ccttttctcc acttggctaa
    11641 atgtaaaaaa atggttaaga ctatgatcag gtcagaccac tttatttatt attttacagg
    11701 ggagtgttat gggtcaggtt ttaccgctgg ttacccgcca gggcgatcgt atcgccattg
    11761 ttagcggttt acgtacgcct tttgcccgcc aggcgacggc ttttcatggc attcctgctg
    11821 ttgatttggg gaagatggtg gtaggcgaac tgctggcacg cagcgagatc cccgccgaag
    11881 tgattgaaca actggttttt ggtcaggtcg tacaaatgcc tgaagccccc aacattgcgc
    11941 gtgaaattgt tctcggtacc ggaatgaatg tgcataccga tgcttacagc gtcagccgcg
    12001 cttgcgctac cagtttccag gcgattgcaa acgtcgcaga aagcctgatg gcgggaacta
    12061 ttcgcgcggg gattgccggt ggggccgatt cctcttccgt attgccaatt ggcgtcagta
    12121 aaaaactggc gcgcgtgctg gttgatgtca acaaagctcg aaccatgagc cagcgcctga
    12181 aactcttctc tcgcctgcgt ttgcgcgact taatgccggt gcctccggca gtggcggaat
    12241 actctactgg cttgcggatg ggcgacaccg ctgaacaaat ggcgaaaacc tacggtatta
    12301 ctcgcgaaca gcaagatgca ttagcgcacc gttcgcatca gcgagccgct caggcgtggt
    12361 cagacggtaa actcaaagaa gaggtgatga ctgcctttat ccctccttat aaacaaccgc
    12421 ttgccgaaga caacaatatt cgcggtaatt cctcgcttgc tgattacgca aagttgcgtc
    12481 cggcgtttga tcgtaaacac gggacggtaa cggcagcaaa cagtacgccg ctgaccgatg
    12541 gcgcagcggc ggtgatcctg atgaccgaat cgcgggcgaa agaattaggg ctggtaccgc
    12601 tggggtatct gcgcagttac gcatttactg cgatagatgt ctggcaggac atgttgctcg
    12661 gtccggcctg gtcaacgccg ctggcgctgg aacgtgccgg tttgacaatg agcgatctga
    12721 cattgctcga tatgcacgaa gcctttgcag ctcagacact ggcgaatatt cagttgctgg
    12781 gtagcgaacg ttttgctcgt gatgtactgg ggcgtgcaca tgccactggc gaaatggacg
    12841 atagcaaatt taacgtgctt ggcggttcga ttgcttacgg gcatcccttc gcggcgaccg
    12901 gcgcgcggat gattacccag acattgcatg aacttcgccg tcgcggcggt ggatttggtt
    12961 tagtgaccgc ctgtgctgcg ggtgggcttg gcgcggcaat ggttgtggag gcggaataat
    13021 ggaaatggca tcagcgttta cccttaatgt ccgtctggac aacattgctg ttatcaccat
    13081 cgacgtaccg ggtgagaaaa tgaataccct gaaggcggag tttgcctcgc aggtgcgcgc
    13141 cattattaag caactccgtg aaaacaaaga gttgcgcggc gtggtgttta tctccgcgaa
    13201 accagacaac ttcattgctg gcgcagacat caacatgatc gccaactgca aaacggcgca
    13261 agaagcggaa gcactggcgc ggcagggcca acagttgatg gcggagattc atgctttgcc
    13321 cgttccggtt atcgcggcta ttcatggcgc ttgcctgggc ggtgggctgg agttggcgct
    13381 ggcgtgccac ggtcgcatgt gtactgacga tcctaaaacg gtgctcggtt tgcctgaagt
    13441 acaacttgga ttattacccg gttcaggcgg cacccagcgt ttaccgcgcc tgataggtgt
    13501 cagcacagca ttggagatga tcctcactgg aaaacaactt cgggcgaaac aggcattaaa
    13561 gctggggctg gtggatgacg tcgttccgca atccatttta ctggaagccg ctgttgagct
    13621 ggcaaagcag gatcgcccat cttcacgccc tctacctgta cgcgagcgta ttctggcggg
    13681 gccgttaggt cgtgcgctgc tgttcaaaat ggtcggtaag aaaacagaac acaaaactca
    13741 aggtaactat ccggcgacag aacgcatcct ggaggttgtc gaaacgggat tagcgcaggg
    13801 caccagtggc ggctatgacg ctgaagcccg tgcttttggc gaactggtga tgacgccgca
    13861 atcgcaggcg ctgcgtagta tcttttttgc cagtacggac gtgaagaaag atcctggcag
    13921 tgatgcgccg cctgcgccat taaacagcgt gggaatttta ggtggtggct tgatgggcgg
    13981 cggtattgct tatgtcactg cttgtaaagc ggggcttccg gtcagaatta aagatatcaa
    14041 cccgcagggc ataaatcatg cgctgaagta cagttgggat cagctggagg gcaaagttcg
    14101 ccgtcgtcat ctcaaagcca gcgaacgtga cagacagctg gcattaatct ccggaacgac
    14161 ggactattgc ggctttgccc atcgcgatct gattattgaa gcggtgtttg aaaatctcga
    14221 attaaaacaa caaatggtgg cggaagttga gcaaaattgc gccgctcata ccatctttgc
    14281 ttcgaacacg tcatctttac cgattggcga tatcgccgct cacgccgcgc gacctgagca
    14341 agttattggc ctgcatttct tcagtccggt ggaaaaaatg ccgttggtag aaattattcc
    14401 ccatgccggt acatcggcgc aaaccatcgc caccacagta aaactggcga aaaaacaggg
    14461 caaaacgcca attgtcgtgc gtgacaaagc cgggttttac gtcaatcgca tcttagcgcc
    14521 ttacattaat gaagctatcc gcatgttgac cgaaggtgaa cgggtagagc acattgatac
    14581 cgcgctggtg aaatttggtt ttccggtagg cccaatccaa cttttggatg aggtaggaat
    14641 cgacaccggg actaaaatta ttcctgtact ggaagccgct tatggagaac gttttagcgc
    14701 gcctgcaaat gttgtttctt caattttgaa cgacgatcgc aaaggcagaa aaaatggccg
    14761 gggtttctat ctttatggtc agaaagggcg taaaagcaaa aaacaggtcg atcccgccat
    14821 ttacccgctc attggcacac aagggcaggg gcgactctcc gcaccgcagg tagctgaacg
    14881 gtgtgtgatg ttgatgctga atgaagcagt acgttgtctg gacgagcagg ttatccgtag
    14941 cgtgcgtgac ggagatattg gcgcggtatt tggcattggt tttccgccat ttctcggtgg
    15001 tccgttccgc tacatcgatt ctctcggtgc gggtgaagtg gttgcaataa tgcaacgact
    15061 ggccacgcag tatggttccc gttttacccc ttgcgagcgt ttagtcgaga tgagcaagcg
    15121 tggggaaagt ttttggaaaa caactgcaac tgacctgcaa taagaaggtc aaagctatat
    15181 gaatccgcgc tgaatggcgg agtgttgctc aaaatgtaaa cgcatattga ctatacttac
    15241 gccattgagg taaaaaacag cgtttcattc ggtgaatgga taaggcacaa tgccggccac
    15301 cgttttcttt ctctggtttc agatgaaaga aaacgggcga atctggttaa caaaagcggt
    15361 gcaatatgca agtttttatc atgcgtcacg gcgacgcagc cctcgatgcc gccagtgatt
    15421 cggttcgtcc tctgaccact aacggttgtg acgaatctcg cctgatggcg aactggctga
    15481 aaggtcaaaa agtggaaatc gaacgcgttc tggtgagccc gttcctgcga gccgagcaaa
    15541 cacttgaaga agtaggggat tgtctgaacc tgccttccag tgcagaagtt ctgccggagt
    15601 taacgccctg cggcgatgtt ggtctggtca gtgcctattt gcaggcgttg accaatgaag
    15661 gtgttgcttc ggtactggtg atctcccact tacccttagt cgggtatctg gttgccgagt
    15721 tatgtccggg cgaaacgccg cctatgttta ccacctcggc cattgccagc gtaacgcttg
    15781 atgaaagcgg aaacggcaca ttcaactggc agatgagccc gtgtaatctg aagatggcaa
    15841 aagcgattta atacgcatgc acaaggcccg ctggtgtaat ctatcgggcc tttatttttt
    15901 tctggcaatg ttgaatttat gcaatcttat tgtactaaca atgatccgct tgtgatcgaa
    15961 t
//
LOCUS       DAZUCC010000115        15862 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZUCC010000115 DAZUCC010000000
VERSION     DAZUCC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.115"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            90..1481
                     /gene="narK"
                     /locus_tag="ACHY09_003054"
     CDS             90..1481
                     /gene="narK"
                     /locus_tag="ACHY09_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFS3886000.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLSVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            1874..5617
                     /gene="narG"
                     /locus_tag="ACHY09_003055"
     CDS             1874..5617
                     /gene="narG"
                     /locus_tag="ACHY09_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFS3886001.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLMGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQALDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITSQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            5614..7152
                     /gene="narH"
                     /locus_tag="ACHY09_003056"
     CDS             5614..7152
                     /gene="narH"
                     /locus_tag="ACHY09_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFS3886002.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            7149..7859
                     /gene="narJ"
                     /locus_tag="ACHY09_003057"
     CDS             7149..7859
                     /gene="narJ"
                     /locus_tag="ACHY09_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFS3886003.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            7859..8536
                     /gene="narI"
                     /locus_tag="ACHY09_003058"
     CDS             7859..8536
                     /gene="narI"
                     /locus_tag="ACHY09_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFS3886004.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVQVEYLTRKY
                     QLVRARH"
     gene            complement(8721..8852)
                     /locus_tag="ACHY09_003059"
     ncRNA           complement(8721..8852)
                     /ncRNA_class="other"
                     /locus_tag="ACHY09_003059"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(8899..8983)
                     /locus_tag="ACHY09_003060"
     tRNA            complement(8899..8983)
                     /locus_tag="ACHY09_003060"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8947..8949),aa:Tyr,seq:gta)
     gene            complement(9143..9985)
                     /gene="purU"
                     /locus_tag="ACHY09_003061"
     CDS             complement(9143..9985)
                     /gene="purU"
                     /locus_tag="ACHY09_003061"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFS3886005.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(10035..10493)
                     /gene="ychJ"
                     /locus_tag="ACHY09_003062"
     CDS             complement(10035..10493)
                     /gene="ychJ"
                     /locus_tag="ACHY09_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFS3886006.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAATLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GSKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10606..11511
                     /gene="rssA"
                     /locus_tag="ACHY09_003063"
     CDS             10606..11511
                     /gene="rssA"
                     /locus_tag="ACHY09_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFS3886007.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            11603..12616
                     /gene="rssB"
                     /locus_tag="ACHY09_003064"
     CDS             11603..12616
                     /gene="rssB"
                     /locus_tag="ACHY09_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFS3886008.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            12818..13726
                     /gene="galU"
                     /locus_tag="ACHY09_003065"
     CDS             12818..13726
                     /gene="galU"
                     /locus_tag="ACHY09_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS3886009.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(13871..14284)
                     /gene="hns"
                     /locus_tag="ACHY09_003066"
     CDS             complement(13871..14284)
                     /gene="hns"
                     /locus_tag="ACHY09_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFS3886010.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            14888..15505
                     /gene="tdk"
                     /locus_tag="ACHY09_003067"
     CDS             14888..15505
                     /gene="tdk"
                     /locus_tag="ACHY09_003067"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS3886011.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(15788..>15862)
                     /locus_tag="ACHY09_003068"
     CDS             complement(15788..>15862)
                     /locus_tag="ACHY09_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886012.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT         3832 a         3795 c         4216 g         4019 t
ORIGIN      
        1 aaagttatct tatcgtttga tttacatcaa attgccttta gctacagaca ctaaggtggc
       61 agacatcgaa acgagtatca gaggtgtcta tgagtcactc atccgccccc gaaagggcta
      121 ctggagctgt cattacagat tggcgaccgg aagatcctgc gttctggcaa caacgcggtc
      181 aacgtattgc cagccgcaac ctgtggattt ccgttccctg tctgctgctg gcgttttgcg
      241 tatggatgtt gttcagcgct gttgcggtga acttaccgaa agtcggtttt aattttacga
      301 ccgatcagct atttatgttg acggcgctgc cttcggtttc tggcgcgtta ttacgtgttc
      361 catactcctt tatggttcct atcttcggtg gtcgtcgctg gacggcgttc agcactggta
      421 ttctgattat tccttgcgtc tggctgggtt ttgccgtgca ggatacctcc acgccttata
      481 gcgtcttcat catcatctct ctgctgtgcg gctttgctgg cgcgaacttc gcatccagta
      541 tggcaaacat cagcttcttc tttccgaaac agaagcaggg tggcgcgctg ggtctgaatg
      601 gtggtctggg caacatgggc gtcagcgtca tgcagttggt tgctccgctg gtggtatcac
      661 tgtcgatttt cgcagtattt ggtagccagg gtgtcaaaca gccggatggg actgagctgt
      721 atctggcgaa tgcgtcctgg gtatgggtgc cgttccttgc catcttcacc attgcggcgt
      781 ggtttggcat gaacgatctt gctacctcga aagcctccat caaggagcag ttgtcggtac
      841 tcaaacgggg tcatctgtgg attatgagcc tgctgtatct ggcaaccttc ggctccttca
      901 tcggcttctc cgcgggcttt gcgatgctgt caaaaacgca gttcccggat gttcagattc
      961 tgcaatacgc tttcttcggg ccgtttattg gtgcgctggc gcgttctgca ggtggtgcat
     1021 tatctgaccg tctgggcgga actcgtgtca cactggtgaa ctttatcctg atggcgattt
     1081 tcagcggcct gctgttcctg accttaccga ctgacggaca gggcggaagc ttcatggcgt
     1141 tcttcgcagt cttcctggcg ctgttcctga cagctgggct gggtagtggt tccacttttc
     1201 agatgatttc cgtgatcttc cgtaaactga caatggatcg cgtgaaagca gaagggggct
     1261 ctgacgaacg tgcgatgcgt gaagcggcaa ccgacacggc ggcggcgctg ggtttcatct
     1321 ctgcgattgg cgcgattggt ggcttcttta tcccgaaagc gttcggtagc tcgctggcat
     1381 taacgggttc gccagtcggc gcaatgaaag tatttttgat tttctatatc gcctgcgtag
     1441 tgattacctg ggcggtatat ggtcggcatt ctaaaaaata aatcttaata tttgattatc
     1501 ctttgcgcgg catgatgtcg cgcttttttt acctgtcatt tagttacaac atccttatga
     1561 agtgtggcgt atttcgccgg ttttcattaa gagccattaa tatgttaccc atggggaata
     1621 ctccttaata cccatctgca taaaaatctc aatagtctaa ataactatgg atgcaaaata
     1681 tcttaattta cagtgattta tgttgaattt attaattatc cttaaggggt atcttaggaa
     1741 tttactttat ttttcatccc catcactctt gatcgttatc aattcccacg ctgtttcaga
     1801 gcgttacctt gcccttaaac attagcaatg tcgatttatc agagagccga caggctccca
     1861 caggagaaaa ccgatgagta aattcctgga ccggtttcgc tacttcaaac agaagggcga
     1921 aacctttgcc gatgggcatg gccagcttct caataccaac cgtgactggg aggatggata
     1981 tcgccagcgt tggcagcatg acaaaatcgt ccgctctacc catggggtaa actgcaccgg
     2041 ctcctgcagc tggaaaatct acgttaaaaa cggtctggtc acctgggaaa cccagcagac
     2101 tgactatccg cgtactcgcc cggatctgcc aaaccatgaa cctcgcggct gcccgcgcgg
     2161 tgccagctac tcctggtatc tttacagcgc caaccgtctg aaatacccga tgatgcgcaa
     2221 acgcctgatg aaaatgtggc gtgaagcgaa ggcgctgcat agcgatccgg ttgaggcatg
     2281 ggcttctatc attgaagacg ccgataaagc gaaaagcttt aagcaggcgc gtggacgcgg
     2341 tggttttgtt cgttcttcct ggcaggaagt gaacgaactg atcgccgcat ctaacgttta
     2401 caccatcaaa aactacggcc cggaccgtgt cgctggcttc tcgccaattc cggcaatgtc
     2461 gatggtttct tacgcgtcgg gtgcgcgtta tctctcgctg cttggcggta cttgcttaag
     2521 cttctacgac tggtactgcg atctgcctcc tgcgtctcca caaacctggg gcgagcaaac
     2581 tgacgtaccg gaatctgctg actggtacaa ctccagctac atcatcgcct gggggtcaaa
     2641 cgtgccgcag acgcgtactc cggatgctca cttctttact gaagtgcgtt acaaaggcac
     2701 caaaaccgtt gccgtcacgc cggactacgc tgaaatcgcc aaactgtgcg atctgtggct
     2761 ggcaccgaag cagggcaccg acgcagcaat ggcgctggcg atgggtcacg tgatgctgcg
     2821 tgaattccac ctcgacaacc caagccagta tttcaccgac tatgtgcgtc gttataccga
     2881 catgccaatg ctggtgatgc tggaagagcg cgacggttac tacgctgcgg gtcgtatgct
     2941 gcgcgctgct gatctggtgg atgcgctggg ccaggaaaac aatccggaat ggaaaactgt
     3001 cgcctttaat accaatggag aaatggttgc gccgaacggt tctattggct tccgctgggg
     3061 cgagaagggc aaatggaatc ttgagcagcg cgacggcaaa actggcgaag aaaccgagct
     3121 gcaactgagc ctgatgggta gccaggatga gatcgctgaa gtgggcttcc catactttgg
     3181 tggcgacggc acggaacact tcaacaaagt ggaactggaa aacgtgctgc tgcacaagct
     3241 gccggtgaaa cgcctgcaac tggctgatgg cagcactgct ctggtgacca ccgtttatga
     3301 tctgacgttg gcaaactacg gtctggaacg tggcctgaac gacgttaact gcgcaaccag
     3361 ctatgacgac gtgaaagcct acacccctgc atgggccgag cagatcactg gcgtttctcg
     3421 cagccagatt atccgcattg cccgtgaatt tgccgataac gctgacaaga cgcacggtcg
     3481 ttcgatgatt atcgtcggtg cggggctgaa ccactggtat cacctcgata tgaactatcg
     3541 tggtctgatc aacatgctga ttttctgcgg ctgtgtaggt cagagcggtg gtggctgggc
     3601 gcactatgta ggtcaggaaa aactgcgtcc gcaaaccggc tggcagccgc tggcgtttgc
     3661 tcttgactgg cagcgtccgg cgcgtcacat gaacagcacc tcttatttct ataaccactc
     3721 cagccagtgg cgttatgaaa ccgtgaccgc agaagagttg ttgtcgccga tggcggataa
     3781 atcccgttat accggacatc tgatcgactt taacgtccgt gcagaacgta tgggctggct
     3841 gccgtctgcg ccgcagttag gcactaaccc gctgactatc gctcgcgaag cagaaaaagc
     3901 cgggatgaat ccggtggact atacggtgaa atccctgaaa gagggttcca tccgttttgc
     3961 ggcagaacaa ccagaaaacg gtaaaaacca cccgcgtaac ctgttcatct ggcgttctaa
     4021 cctgctcggt tcttccggta aaggtcatga gtttatgctc aagtacctgc tggggacgga
     4081 gcacggtatc cagggtaaag acctgggtca gcagggcggc gtgaagccgg aagaagtgga
     4141 ctggcaggac aacggtctgg aaggcaagtt ggatctggtg gtgacgctgg acttccgtct
     4201 gtcgagcacc tgtctctatt ccgacatcat tttgccgacg gcgacctggt acgaaaaaga
     4261 cgacatgaat acttcggata tgcatccgtt tattcacccg ctgtctgctg cggtcgatcc
     4321 ggcctgggaa gcgaaaagcg actgggaaat ctacaaagcc atcgcgaaga aattctccga
     4381 agtgtgcgtt ggtcatctgg gcaaagaaac cgacatcgtc acgctgccta tccagcacga
     4441 ctctgccgct gaactggcgc aagcgctgga tgtgaaagac tggaaaaaag gcgaatgtga
     4501 cctgatccca ggtaaaaccg caccgcacat tatggttgta gagcgcgatt atccggcaac
     4561 ttacgaacgc tttacctcta tcggcccgct gatggagaaa atcggtaacg gcggtaaagg
     4621 gattgcctgg aacacccaga gcgagatgga tctgctgcgt aagctcaact acaccaaagc
     4681 ggaaggtccg gcgaaaggcc agccgatgct caacaccgca attgatgcag cagagatgat
     4741 cctgacgctg gcaccagaaa ccaacggcca ggtggccgtg aaagcctggg cggcattgag
     4801 tgagtttacc ggtcgtgacc atacgcatct ggcgctgaat aaagaagacg agaagatccg
     4861 cttccgtgac attcaggcgc agccgcgcaa aatcatctcc agcccgacct ggtctggcct
     4921 tgaagatgaa catgtttctt acaacgccgg ttacaccaac gttcacgagc tgatcccgtg
     4981 gcgtacgctc tctggccgtc agcagctgta tcaggatcat cagtggatgc gtgatttcgg
     5041 tgaaagcctg ctggtttatc gtccgccgat cgacacccgt tcggtgaaag aagtgatggg
     5101 ccagaaatct aacggtaatc cggaaaaagc gctcaacttc ctgacaccgc accagaagtg
     5161 gggtatccac tccacctaca gcgacaacct gctgatgctg actttaggtc gcggtggtcc
     5221 ggtggtctgg ttgagtgaag ccgatgccaa agagctgggt atcgccgaca acgactggat
     5281 tgaagtcttc aacagcaacg gtgcattgac tgcccgtgcg gttgtcagcc agcgtgttcc
     5341 ggcagggatg accatgatgt accacgcgca ggaacgtatc gttaacctgc cgggttcaga
     5401 aatcaccagc cagcgcggtg gtattcataa ctcggtcacc cgtatcacgc cgaaaccgac
     5461 gcatatgatc ggcggctatg cccatctggc atacggcttt aactactacg gcactgtagg
     5521 ttctaaccgc gatgaatttg ttgtagtgcg taagatgaag aacattgact ggttagatgg
     5581 cgaaggcaat gaccaggtac aggagagcgt aaaatgaaaa ttcgttcaca agtcggcatg
     5641 gtgctgaatc tggataagtg catcggttgc cacacctgtt cagtgacctg taaaaacgtc
     5701 tggaccagcc gtgaaggcgt ggaatacgcg tggttcaaca acgtggaaac caagccgggc
     5761 cagggcttcc cgacagactg ggaaaaccag gaaaaataca aaggcggctg gatccgtaaa
     5821 atcaacggca aactgcaacc gcgcatgggt aaccgcgcca tgctgctggg taaaattttc
     5881 gctaacccgc atctgccggg gatcgacgat tattacgagc cgttcgattt tgactatcag
     5941 aacctgcata ccgcgccgga aggcagcaaa tcgcagccga ttgcccgtcc acgttcgctg
     6001 attaccggcg aacggatggc gaaaatcgaa aaagggccaa actgggaaga tgacctgggc
     6061 ggcgagtttg acaaactggc gaaagacaag aacttcgaca acatccagaa ggcgatgtat
     6121 agccagttcg aaaacacctt catgatgtat ttgccacgcc tgtgcgaaca ctgcctgaac
     6181 ccggcatgtg tggcgacctg cccgagcggt gcgatttaca agcgtgaaga agatggcatc
     6241 gtcctgatcg accaggacaa atgccgtggc tggcgtatgt gcatcaccgg atgcccgtac
     6301 aaaaaaatct acttcaactg gaagagcggt aagtctgaga agtgcatctt ctgctatccg
     6361 cgtattgaag ccggtcagcc gacggtgtgc tctgaaacct gtgtcggtcg tatccgttat
     6421 cttggcgtgc tgctgtacga tgccgacgcg attgaacgtg cagccagcac cgagaacgag
     6481 aaagatctct accagcgtca gctggacgtg ttcctcgatc cgaacgatcc gaaggtcatc
     6541 gagcaggcga ttaaagacgg tattccgctg agcgttattg aagccgcaca gcagtcgccg
     6601 gtttataaaa tggcgatgga atggaaactg gcgctgccgc tgcatccgga atatcgcacg
     6661 ctgccgatgg tctggtatgt gccacctctg tcgccgattc agtctgcggc agatgcgggt
     6721 gagctgggca gcaatggcat tctgccagac gtcgaaagcc tgcgtattcc ggtacagtat
     6781 ctggcgaatc tgctgaccgc aggtgatacc aaaccggtac tgcgcgcgct gaaacgtatg
     6841 ctggcgatgc gtcattataa acgtgctgaa accgttgacg gtaaagttga tacccgtgcg
     6901 ctggaagagg tcggtctgac cgaagcccag gcacaggaga tgtaccgtta tctggcgatt
     6961 gctaactacg aagatcgctt tgtggtgccg agcagtcatc gtgaactggc gcgggaagcc
     7021 ttcccggaga aaaatggctg cggctttacc tttggtgatg gctgccacgg ctcagatacc
     7081 aaattcaatc tgtttaacag ccgtcgtatc gatgccatcg acgtgaccag taaaacggag
     7141 ccgcacccat gatcgaactc gtgattgtat cgcgtctcct tgaatatccg gatgctgcct
     7201 tatggcagca tcaacaagag atgtttgagg cgattgccgc gtcgaaaaat ctgccaaaag
     7261 aggatgccca tgcgctgggc attttcctgc gcgatttaac gacgatggat ccgctcgatg
     7321 cccaggcgca gtacagcgaa ctgttcgacc gtggccgcgc cacgtcactg ttgctatttg
     7381 aacatgttca cggtgaatcc cgtgaccgcg gccaggcgat ggtggatctg ctggcgcagt
     7441 acgagcagca cggcttgcag ttaaacagcc gcgaattgcc cgaccatctg ccgctgtatc
     7501 tggagtacct ggcgcagctg ccgcaaagcg aagccgtgga gggtttgaaa gatattgcgc
     7561 cgattctggc attgctgagc gcgcgtctgc aacagcgtga aagccgttat gccgtgctgt
     7621 ttgatctgct gctgaaactg gcgaataccg ccatcgacag cgacaaagtg gcggaaaaaa
     7681 ttgccgatga agcgcgcgac gatacgccgc aggcgctgga tgcagtctgg gaagaagagc
     7741 aggttaaatt ctttgctgaa aaaggctgcg gtgattcagc aatcactgct catcagcgtc
     7801 gctttgccgg tgccgtcgcg ccgcaatatc tgaatatcac caccggagga cagcactaat
     7861 gcaattcctg aatatgttct tctttgatat ctacccgtac atagccgggg cggtcttcct
     7921 gattggtagc tggctgcgtt atgactacgg gcagtacacc tggcgcgcgg cgtccagcca
     7981 gatgctggat cgcaaaggga tgaacctggc gtcgaacctg ttccatatcg gtattctggg
     8041 gatttttgtt ggtcacttct tcggtatgct gacgccgcac tggatgtatg aagcctggct
     8101 gccgattgaa gtgaaacaga aaatggcgat gtttgctggt ggtgccagcg gtgtgctgtg
     8161 tctgattggc ggcgtgctgt tgctgaagcg tcgtctgttc agcccacgcg tgcgtgcaac
     8221 cactaccgga gcggatatcc tgatcctgtc gctgctcgtt atccagtgcg cgctgggcct
     8281 gttgaccatt ccgttctccg cccaacatat ggacggtagc gagatgatga aactggttgg
     8341 ctgggcgcag tcggtagtga ccttccacgg tggcgcttct caacaccttg atggtgtggc
     8401 gtttatcttc cgtctgcacc tggtgttggg gatgacgtta ttcctgctgt tcccgttctc
     8461 gcgtctggtg cacatctgga gcgtacaggt ggagtatctg acacgtaagt atcagctggt
     8521 gcgcgctcgt cactaagcga attttagttt gcatagaccc cgcttcggcg gggttttttt
     8581 atgggcacgg tacggggtga gttgtcggat gcgcttcgct tatccgacct acaggggagg
     8641 atattgtagg tccggtaaac gcggtgccat tgggcagaat tagctcggag taacaggttt
     8701 tgagtattta caggaagtga tggtggtgga ggaaggatta ctcagcgctg cgcgcttcgc
     8761 ccttcgggtc gttgcctgcg gccacgctct ctcgctggcg ctcgagtcga accctggtcg
     8821 aagcttctca tccttccccg cttgggcaga atatttaatc gcggattcgt ttgagagttc
     8881 acggactttt gaaagtgatg gtggtggggg aaggattcga accttcgaag tcgatgacgg
     8941 cagatttaca gtctgctccc tttggccgct cgggaacccc accacggggt aatgcttttt
     9001 actagcctgc tcccttatcg ggaagcgggg cgcatcatat caaatgacgc gccgctgtaa
     9061 agtgttacgt tgagaaaaat gaactggttg cgtaattttc acccgtaacg gattaaaggt
     9121 aaccagttat ttttgctggc gattaaagaa taatcgtccg attaccgtaa acaaagacgc
     9181 gctgcgccag tactttgtag agtgcgcgac ttaagacgtt tttctcgacg tcacgacctg
     9241 cgcgcatcat atcttcagct gtgtaggtat gatcgacatg aataacgtcc tgcatgatga
     9301 ttggaccttc gtccagattg tcattcacat agtgagcggt agcgccaata atcttcacgc
     9361 cacgttcata ggcctggtga taaggacgtg cgccgataaa cgctggcagg aatgaatggt
     9421 gaatattgat gatcttattc gggaagcgtg ccacaaattc tggcgttaat acccgcatat
     9481 acttcgccag caccacgtag tcaggctgat aagcatcaat ggcatccgcc atcttttgat
     9541 cgtgctcgtt gcggcttaac ccttcatggc ttaccagctc aaacggaata tcaaaacgct
     9601 caaccagaga acgtaaagta tcgtggttac caataactgc cgcgatttcg acatccaggc
     9661 cgccgtaatt ggctttcatc aacaaatcgc caaggcaatg cgcctcttta gtgaccagaa
     9721 tcactatccg gcgacgaccg gcaggattca gctcacgtac ggagccttct ggcaatgcgc
     9781 tgtcgagatc cgccagcagg gtggaatcat taaaaattcc ttccagttcc gtgcgcataa
     9841 aaaagcgccc ggtacggtga tcaacaaatt cattgttctg tacgatattt aactcgtgct
     9901 tgtagcaaat attggtaata cgtgcgatca gacctttttg gtccggacaa atagtacgca
     9961 gaactttacg ttggagtgaa tgcattgctg gaaaaacctt gttgagagtg tttgctaaac
    10021 cgtaccgtca accattattg gccgcagcac tttttaaatt ttttacctga accacaaggg
    10081 cagggatcgt tgcggccaaa ctgcggacgt gtaccgtcaa tatagtacca ttgaccgttt
    10141 tctttcaaaa atcgcgaacg ttcaattatt gcacctgttt tgctaccttc ggtaaatcgc
    10201 gcaacaaaac tgacaaatcc gatattgtcg acgtcctgcc aacaatgttc aaatacggtc
    10261 aatcccaacc attcagtatg cgcaaatccg gcaatcaatt cggcacgtaa cgtcgccgct
    10321 ccacaagatg gatgccaggt ctttattaaa taatctgcgt ctttcatcac aaaagcgcag
    10381 taacgcgaac gcatgagatg ttctggatca ggtgcaacct tttcaccaga cacataaggg
    10441 tggcaacata ggctatactc gacagcacta ccacagggac aaagctgaga cacaaataat
    10501 ctccctggaa acaataacgg cgtattaacc gcctgagtag cactatgtta accgagcagt
    10561 agcgatgttg ctacgattgc attccagggg aatcttgcgg gaataatgag aaagataaaa
    10621 atagggctgg cgctgggatc tggcgcggcg agaggttggt cgcatattgg cgttattaat
    10681 gcgctaaaaa aagtgggtat tgaaattgat atcgttgcag gatgttcaat tggttcgctg
    10741 gtgggcgctg cctatgcatg cgatcgacta tctgcgctgg aagattgggt gacctctttc
    10801 agttattggg atgttttacg cctgatggat ctctcctggc agcgcggtgg gttactgcgc
    10861 ggcgagcgtg tcttcaatca atatcgcgaa ataatgccgg aaacagagat cgaaaattgt
    10921 tcccgtcgct tcgcggctgt tgccaccaat ttaagtactg ggcgcgaatt atggtttact
    10981 gaaggcgatc tccatcttgc tattcgtgca tcatgcagta ttccaggact aatggcaccc
    11041 gttgcgcata acggctactg gctggttgat ggcgctgtag ttaacccaat tcctatttcc
    11101 ctcacgcgtg cattgggggc tgatattgtg atagcggtcg acctgcagca cgatgctcat
    11161 ttgatgcaac aagatttgct ctcctttaat gtcagtgaag aaaatagcga gaatggtgat
    11221 tctctgccgt ggcatgcgcg tctgaaagaa aggttaggca gcataacgac acgtcgggcg
    11281 gtgacagcgc caacggcaac agagattatg accacttcta tccaggtgct ggagaaccgc
    11341 cttaaaagga accgcatggc aggtgatccg cccgatattc tgattcaacc tgtttgcccg
    11401 caaatatcta cgcttgattt ccatcgcgcg cacgctgcca ttgcggccgg gcagctggca
    11461 gtggaaaaga aaatggacga acttttgccg ttggtacgca ccaacatttg accagaattt
    11521 ttatctacac ttaagttaat tctgacaggc gcaggtggca atagcatgcc actattgagt
    11581 aaagccagtc aggggagaga acatgacgca gccattggtc ggaaaacaga ttctcattgt
    11641 tgaagatgag caggtatttc gctcgcttct ggattcgtgg ttttcctcat tgggagcgac
    11701 aacggtactg gcggctgatg gggtggatgc ccttgagttg ctgggaggtt tcactccaga
    11761 cctgatgata tgtgatatcg cgatgccacg aatgaacggg cttaaactgc tggagcatat
    11821 acgtaacaga ggcgaccaga ccccagttct ggtgatatct gccactgaaa atatggcaga
    11881 tattgccaaa gcgttacgtc tgggcgttga agatgttttg ctgaaaccag ttaaagatct
    11941 gaatcgcttg cgcgagatgg tttttgcctg tctctatccc agcatgttta attcccgcgt
    12001 tgaggaagag gaaaggcttt ttcgcgactg ggatgcaatg gttgataacc ctgccgcagc
    12061 ggcgaaatta ttacaggaat tgcaaccgcc ggttcagcag gtgatttccc attgccgggt
    12121 taattatcgt caattggttg ccgcggacaa acccggcctg gtgcttgata ttgccgcact
    12181 ttcggaaaac gatctggcat tttactgcct tgatgtcacc cgagctggac ataatggcgt
    12241 acttgctgcc ttgttattac gcgcattgtt taacggatta ttacaggaac agcttgcaca
    12301 ccaaaatcaa cgtttgccag agttgggcgc gttattgaag caggtaaacc atttactccg
    12361 tcaggccaat ctgccggggc agtttccgct attagttggc tattatcatc gcgaactgaa
    12421 aaatctcatt ctggtttctg cgggtctgaa tgcgacgtta aataccggcg aacaccaggt
    12481 gcaaatcagt aatggtgttc cgttaggcac tttaggtaac gcttatttga atcaattgag
    12541 ccagcgatgc gatgcctggc aatgccaaat atggggaacc ggtggtcggc ttcgcttgat
    12601 gttgtctgca gaatgagcaa acgataacgc gggctaaatt tgcattacct gctaatgtcg
    12661 gctggtggta ctatcgtcgc cattcgtata agtaattgtc ttaattatgc taactcgcct
    12721 ccttttcaga acttagcccc ttcggggtgc tgatatactg ggatgcgata cagaaatatg
    12781 aacacgttca aaacacgaac agtccaggag aatttaaatg gctgccatta atacgaaagt
    12841 caaaaaagcc gttatccccg ttgcgggatt aggaaccagg atgttgccgg cgacgaaagc
    12901 catcccgaaa gagatgctgc cacttgtcga taagccatta attcaatacg tcgtgaatga
    12961 atgtattgcg gctggcatta ctgaaattgt gctggttact cactcatcta aaaactctat
    13021 tgaaaaccac tttgatacca gttttgaact ggaagcaatg ctggaaaaac gtgtaaaacg
    13081 tcaactgctt gatgaagtgc agtctatttg tccgccgcac gtgactatta tgcaagttcg
    13141 tcaggggctg gcgaaaggcc tgggacatgc ggtattgtgt gctcacccgg tagtgggtga
    13201 tgaacctgtg gctgttattt tgcctgacgt tattctggat gaatatgaat ccgatttgtc
    13261 acaggataac ctggcagaga tgatccgccg ctttgatgaa acgggtcata gccagatcat
    13321 ggttgaaccg gttgctgatg ttaccgcata tggcgttgtg gattgcaaag gcgttgaatt
    13381 agcgccgggt gaaagcgtac cgatggttgg tgtggttgaa aaaccaaaag cggatgttgc
    13441 gccgtctaat ctcgctattg tgggtcgtta cgtacttagc gcggatattt ggccgttgct
    13501 ggcaaaaacc cctccgggag ctggtgatga aattcagctc accgacgcaa ttgatatgct
    13561 gatcgaaaaa gaaacggtgg aagcctatca tatgaaaggg aagagccatg actgtggtaa
    13621 taaattaggt tacatgcagg ccttcgttga atacggtatt cgtcataaca cccttggtac
    13681 ggaatttaaa gcctggcttg aagaagagat gggcattaag aagtaacatc cgtatcggtg
    13741 ttatccacga aacggcgttg agcaatcgac gccgtttttt tatagcttat tcttattaaa
    13801 ttgtcttaaa ccggacaata aaaaatcccg ccgatggcgg gatttttaag caagtgcaat
    13861 ctacaaaaga ttattgcttg atcaggaaat cgtcgaggga tttaccttgc tcatccattg
    13921 cttttttgat tacagctgga gtacggccct ggccagtcca ggttttagtt tcgccgtttt
    13981 cgtcaacgta gctatatttt gccggacgct gagcacgttt agctttggtg ccagatttaa
    14041 cggcagcaag gctattcagc agttcgttcg ggtcaatacc gtcagcgatc agcatttcgc
    14101 gatattgctg cagtttacga gtgcgctctt caacttcagc agcagccgcg ctttcttctt
    14161 cgcgacgttc gttaacaaca acttctaatt tttccagcat ttcttccagc gtttcaagtg
    14221 tacattctct tgcctgcgca cgaagagtac ggatgttgtt cagaatttta agtgcttcgc
    14281 tcattgtagt aatctcaaac ttatattggg gtggtttgtt gaggtaataa tagagcctta
    14341 aattcagttg tgcaatagcc aggaatgtaa ggaattcaaa attgttcttt attttgtgcc
    14401 gccaataaat atcttttcat aaaattagcc agaaaagacg cggcatatag ccctatttgc
    14461 gccgatgatt tcgcagcacg tgaggttaaa acttcctgat tcatgtcaca ttttatgggg
    14521 agattatcgt aggctgacga cctttcagtc ttctgtatta gtcgtgttta caagaattcc
    14581 ctattaagcg aatgatgaaa agtagaacag tcgcaataag agcagggact tagtattgca
    14641 ctatctcctg gaggtcaaca gagggctatt acttgcgcaa caggttaaag attgtgaata
    14701 gttaccagca gtcatttacc cgcttataac aagcgaggca gttgtaatga tagctcagaa
    14761 ggattatgca aggcttcgta agggagaacg catataccac ttctgtgcat actgttgagc
    14821 tgaaaaactg acgaattatg ataaactcca gccaacttta attcatatca ttgagggcct
    14881 gtggctgatg gcacagctat atttctacta ttccgcaatg aatgcgggta agtctacagc
    14941 attgttgcaa tcttcataca attaccagga acgcggcatg cgcactgtcg tatatacggc
    15001 agaaattgat gatcgctttg gtgccgggaa agtcagctcg cgtataggtt tgtcatcgcc
    15061 tgcaaaatta tttaaccaaa attcatcatt atttgatgag attcgtgcgg aacatgaaca
    15121 gcaggcaatt cattgcgtac tggttgatga atgtcagttt ttaaccagac aacaagtata
    15181 tgaattatcg gaggttgtcg atcaactcga tatacccgta ctttgttatg gtttacgtac
    15241 cgattttcga ggtgaattat ttattggcag ccaatactta ctggcatggt ccgacaaact
    15301 ggttgaatta aaaaccatct gtttttgtgg ccgcaaagca agcatggtgc tgcgtcttga
    15361 tcaagcaggc agaccttata acgaaggtga gcaggtggtt attggtggta atgaacgcta
    15421 cgtttctgta tgccgtaaac actataaaga agcgttagaa gtcggctcat taacggctat
    15481 tcaggaaagg catcgccacg attaataaga atttctttac tgacagggtg agcagggcgc
    15541 ttttatcctg tcagttcgtt ttactcactt tatccggtgt atataccctt ctcgacagtt
    15601 ctttaacgcc gctatacccc tcacagagcg cttttacagt gacgttgact tgtgacctgt
    15661 aaagtacaat atcgctgtgt gtaggcgtta tacatcgccg taaatatgat gcaggcaaat
    15721 gctgtcggtt tatgggaaag ttgctttggg taaaaaaaac ggccccagaa gggataatgc
    15781 cagtcagtta agcaactgac tggctctttt tcggggctgt ggggtatttc cagggcctct
    15841 cctttaccac tctcgggaag gc
//
LOCUS       DAZUCC010000116        15845 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZUCC010000116 DAZUCC010000000
VERSION     DAZUCC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.116"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..193
                     /gene="yecU"
                     /locus_tag="ACHY09_003069"
     CDS             17..193
                     /gene="yecU"
                     /locus_tag="ACHY09_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFS3886013.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            180..404
                     /gene="yecF"
                     /locus_tag="ACHY09_003070"
     CDS             180..404
                     /gene="yecF"
                     /locus_tag="ACHY09_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFS3886014.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(471..1193)
                     /gene="sdiA"
                     /locus_tag="ACHY09_003071"
     CDS             complement(471..1193)
                     /gene="sdiA"
                     /locus_tag="ACHY09_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFS3886015.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCTAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1423..2175)
                     /gene="tcyN"
                     /locus_tag="ACHY09_003072"
     CDS             complement(1423..2175)
                     /gene="tcyN"
                     /locus_tag="ACHY09_003072"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFS3886016.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(2172..2840)
                     /gene="tcyL"
                     /locus_tag="ACHY09_003073"
     CDS             complement(2172..2840)
                     /gene="tcyL"
                     /locus_tag="ACHY09_003073"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFS3886017.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2855..3841)
                     /gene="dcyD"
                     /locus_tag="ACHY09_003074"
     CDS             complement(2855..3841)
                     /gene="dcyD"
                     /locus_tag="ACHY09_003074"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFS3886018.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKIGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3946..4746)
                     /gene="tcyJ"
                     /locus_tag="ACHY09_003075"
     CDS             complement(3946..4746)
                     /gene="tcyJ"
                     /locus_tag="ACHY09_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3886019.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4834..5385)
                     /gene="fliZ"
                     /locus_tag="ACHY09_003076"
     CDS             complement(4834..5385)
                     /gene="fliZ"
                     /locus_tag="ACHY09_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFS3886020.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5431..6150)
                     /gene="fliA"
                     /locus_tag="ACHY09_003077"
     CDS             complement(5431..6150)
                     /gene="fliA"
                     /locus_tag="ACHY09_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFS3886021.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6469..8208)
                     /locus_tag="ACHY09_003078"
     CDS             complement(6469..8208)
                     /locus_tag="ACHY09_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFS3886022.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAATKSDLAAAQLLAPGTADANGTVTYTVSAGLKT
                     STAADVIASLANNAKVNATIANGFGSPTATDYTYNSATGDFTYSATIAAGTNSGDSNS
                     AQLQSFLTPKAGDTANLNVKIGSTSIDVVLASDGKITAKDGSELFIDVDGNLTQNNAG
                     TVKAATLDALTKNWHTTGTPGAVSTVITTEDETTFTLDGGTDATTSGAITVANARMSA
                     ESLQSATKSTGFTVDVGATGTSAGDIKVDSKGIVQQYTGTVFEDAYTKADGSLTTDNT
                     TNLFLQKDGTVTNGSGKAVYVSADGNFTTDAETKAATTADPLKALDEAISSIDKFRSS
                     LGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKA
                     NQVPQQVLSLLQG"
     gene            8474..9880
                     /gene="fliD"
                     /locus_tag="ACHY09_003079"
     CDS             8474..9880
                     /gene="fliD"
                     /locus_tag="ACHY09_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFS3886023.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLIIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            9905..10315
                     /gene="fliS"
                     /locus_tag="ACHY09_003080"
     CDS             9905..10315
                     /gene="fliS"
                     /locus_tag="ACHY09_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFS3886024.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPD"
     gene            10315..10680
                     /gene="fliT"
                     /locus_tag="ACHY09_003081"
     CDS             10315..10680
                     /gene="fliT"
                     /locus_tag="ACHY09_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFS3886025.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10758..12245
                     /gene="amyA"
                     /locus_tag="ACHY09_003082"
     CDS             10758..12245
                     /gene="amyA"
                     /locus_tag="ACHY09_003082"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFS3886026.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(12279..12692)
                     /gene="yedD"
                     /locus_tag="ACHY09_003083"
     CDS             complement(12279..12692)
                     /gene="yedD"
                     /locus_tag="ACHY09_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFS3886027.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     SALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12879..14084
                     /gene="yedE"
                     /locus_tag="ACHY09_003084"
     CDS             12879..14084
                     /gene="yedE"
                     /locus_tag="ACHY09_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFS3886028.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            14081..14314
                     /gene="yedF"
                     /locus_tag="ACHY09_003085"
     CDS             14081..14314
                     /gene="yedF"
                     /locus_tag="ACHY09_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFS3886029.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            14423..15091
                     /gene="yedK"
                     /locus_tag="ACHY09_003086"
     CDS             14423..15091
                     /gene="yedK"
                     /locus_tag="ACHY09_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFS3886030.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAVREWMRQEVGGKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAAL
                     IQPV"
     gene            15202..15681
                     /gene="yedL"
                     /locus_tag="ACHY09_003087"
     CDS             15202..15681
                     /gene="yedL"
                     /locus_tag="ACHY09_003087"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3886031.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PKVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELP"
BASE COUNT         3933 a         4038 c         3874 g         4000 t
ORIGIN      
        1 cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg tattttatag
       61 cactgccgat atcacgctca aaaaacaacc actgctattt ttagcaaagc ttatggtata
      121 ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc aacatgacga ggaaaataaa
      181 tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat gaagtctcct
      241 gcctgaaagc gatgctgacg ctgatgctgc aggcgatggg acaagctgac gcgggccgcg
      301 tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag gctgcagtat
      361 tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa accggctgag
      421 agcgtgcctt tcagccggtt tttgcatctg gcacgcagaa agaaaagcga tcaaattaag
      481 ccagtagcgg ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat ttttttctgc
      541 atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat cgctatctct
      601 gctgatgtct tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc
      661 atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc
      721 accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgcagtg
      781 caacgggaaa aggacaaaaa gcccagcgcc cggtttggca gcattaaata ctgagtgaca
      841 ccgcggcgta aaccatgcgc gcgcgcagct tcccataacg gctgtgcttc gctgaataag
      901 tcatcattcc acattaaatg gccctgacta aagttttcag ggttcagtac aggatcaatt
      961 gcgagaaagt tttttgcctg ataataacta acccacgcct cagggtaatt ggtgtagaaa
     1021 gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac
     1081 tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc ctccatcctc
     1141 tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac
     1201 cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa
     1261 gatttatata taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct
     1321 cttatgttat taatgagtta tgctgatttg ttaagcagtt ttatcacgtt tgaagtggcg
     1381 caccgccccg acaggtgaat cgtcggggct gattttttct tattattgca gcagaaactt
     1441 ctcgaggaac tggcgggtgc gaggctgctc gggatcggca aataacgctt ttgcggcccc
     1501 ctgctcgact atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa
     1561 gctcatttcg tgcgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt
     1621 gttcaggact tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat
     1681 cacttcagga cgcatagcca gcgctcgcgc aatcgcgaca cgctgctgtt gaccgccaga
     1741 caaacgacgt ggatagctgg tttctttacc tgccagccca actttcgcca gcagctcgcg
     1801 agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat
     1861 gttttccagc accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg
     1921 ctgacgcaac tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatggt
     1981 gatatcgcca acggtgatgg ttcccgcttc cggttgttcc agcagattta tgctgcgtag
     2041 caacgtggtt ttgccggaac cacttggacc gataattgcc accacttcgc caggcttcac
     2101 ctcaaggtcg ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac
     2161 ttcaatggca ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc
     2221 tgcaacgtcg ataatactgt cgccatgatc cagtagatga gcgaagccgc caggtacatg
     2281 gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc
     2341 tggattgtcg cggccagcga cgtatctttt accaggctga taaagctgtt cgacagcggc
     2401 ggcaacgcca cacgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc
     2461 ataccaatac tggcagcagc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc
     2521 agtgtttcgg cggcataggc ggcagtgttt agcgacagac caatcattgc tgacggaatg
     2581 ggatctaatt cgatgccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg
     2641 agtggcgtac cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt
     2701 ggcgaaaggc gcatcagcgc gagaataaac ccgagcagta agccaaaaaa catgccgccg
     2761 atactgagtt gcagcgtata cccagcccct ttcagcagga acggcaagga atcaataacc
     2821 agttgtatac tttcttgcat gagcgtttcc ggagctaaac gtggggatga taggcgaaca
     2881 gcgcaggcgc gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct
     2941 gactgatacc gtcaatcagc cccgccattg cttttccggt atacacagga tcaagcagaa
     3001 tgccttccag ccgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt
     3061 agccaggtgc aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt
     3121 ctttcgcaat cgcctgttgg aggttaacca ctttcggcaa ttgatcggca acggaacgcg
     3181 acacggtcac gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc
     3241 cggcgtgagt tccggcactg cccgacgcga ccaccaccga cgaaatatta actgcccctt
     3301 cacactgttg cgcaatttcc agcgcactct ccacataacc cagcgcgccc aaagcattag
     3361 aaccgccaac cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagct
     3421 cttccagttg ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca
     3481 gatccagcaa caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat
     3541 tttccagcag cgccacgcag tgcagaccga ttttcgccgc aactgcggca gtctggcgca
     3601 catggttaga ctggatcgcc ccggcagtaa tcagcgtatc ggcaccttca cgcagagcat
     3661 ctgccgcgag aaattccagc ttacgtaatt tattgccgcc cattgccatg ggagtgacgt
     3721 catcccgttt gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga
     3781 gcggcgttgg cgcgccgata aactccagcc gtggaaaacg ggttaaatta tgcagtggca
     3841 taacagcctc cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa
     3901 taaaaaaggc gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc
     3961 agcaccaaac catttttcgg aaagggcttg cagagtgcca tctttttgca tttccgcaat
     4021 tgcatcattc accgctttca gcaggtcttc atttccttta cgcagcgcca caccagactc
     4081 ctgacgggag aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc
     4141 cagcgccgcc agacggtcaa cgaggatcgc atcgatacgg cctacgcgca gatcctgata
     4201 tttggtcggg tcatcatcat aggtacgcac atcgacgccc tgaacattct gacgcagcca
     4261 ctcttcatag ttggtgccca gaccgacacc cacttttttg cctttcagat catcagccgt
     4321 tttaatggtg ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtacgg
     4381 ggtagagaaa tcgtattttt tcttgcgctc gtcagaaatg gtgacctggt taatcaccac
     4441 atcaatgcgt ttagagtcca gcgacgccag cataccgtcc catttagtcg gttttagtga
     4501 cgcctcaacg ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa
     4561 tttgccgtca tccccctgaa aactgaacgg cggataagtt ccttccagcc ctaccagcag
     4621 cgtgccgcgc tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat
     4681 acccgcaacc agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa
     4741 tttcatattt accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa
     4801 cacaaaaaca actccgctac atcttattct tatttaatat atatcagaag caggcagact
     4861 ggaggatttc tgcacaagtc cggtacacgt ttgacgctga tagtgttgat acttccgtaa
     4921 cgcaatgcgg taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag
     4981 gaaaccgtcc tgcagctgat cgagggaaat attttgctcg tgtagatgat tacccaaacg
     5041 gcgcagacga acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag
     5101 aaattgctta aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc
     5161 gcaaaatcgg cacaatgccg cccatgattt ttgttccact tgccagccat tttcatcaag
     5221 cagcatgtcc aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaacgagggt
     5281 aatgcgatcg agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt
     5341 aagatagcgg cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta
     5401 gtcaaaatta aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg
     5461 tttaatggcc tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc
     5521 aatctctttg agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc
     5581 cggcaacgtt tcgatagctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg
     5641 tagcgggttt tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc
     5701 gcgccactca tcgtaggaga agagctggct gttattggtg tcgagcaaca tttggcgata
     5761 gtcggcaata tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag
     5821 ttcctgctcc agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg
     5881 cggcacccag tcacggctgc gaagttcatc cagcatagcg ccacggatac gctgcactgc
     5941 gtaagttgta aatgccgttc cttgtagggc gtcatagcgt tcgacggcat tgagtaaccc
     6001 aatgccgccc gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag
     6061 gcgcaatgct tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat
     6121 tacaccttca gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc
     6181 ggcatgatta tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg
     6241 ttatttgggg gttaccggta aattgcgggc agaaaaaacc ccgccgttgg cggggaagca
     6301 cgttgctgac aaattaccat tcatgttgcc ggatgcggcg taaacgcctt atccggccta
     6361 caaaatccgc aaattcaata aattgcaatt caccttgtag gcctgataag cgcagcgcat
     6421 caggcaattt ggcgttgccg tcagtctcag ttaatcaggt tacgacgatt aaccctgcag
     6481 cagagacaga acctgctgcg gtacctggtt agcttttgcc agcacggagt taccggcctg
     6541 ctggatgatc tgcgctttcg acatgttgga cacttcggtc gcatagtcgg cgtcctgaat
     6601 acgggactgc gcttcggaca ggttggtagt ggtgttgttc aggttggtga ccgcggaatc
     6661 cagacggttt tgcaccgcac cgagggagga gcggaatttg tcgatggagc tgatggcttc
     6721 gtccagagct ttcagtggat cggcggtggt tgcagcttta gtttcagcgt cagtagtaaa
     6781 attaccatcc gctgaaacat agactgcttt acctgaacca ttggtcacag ttccgtcttt
     6841 ttgcaaaaac agattggttg tattatcggt agtcagtgaa ccatcagctt tggtgtaagc
     6901 gtcttcaaaa actgtacctg tgtattgttg tactatacct ttactatcaa ctttaatatc
     6961 gcctgcgctg gtaccagtag ctccaacatc aactgtgaat cctgtggact tagttgccga
     7021 ttgaagagac tcagcactca ttcttgcatt tgctacagtg attgcaccag aagtagtagc
     7081 atcagtaccg ccatccagag tgaaggttgt ttcatcttca gttgtaatta ccgtagatac
     7141 ggcacccggt gtgcctgttg tatgccagtt tttagtcagt gcatcaagag tggctgcttt
     7201 gacagtccca gcattgtttt gagtgaggtt accatctacg tcaataaata gttctgaacc
     7261 atctttcgcg gtaattttac cgtcgctagc caatacaacg tcaattgacg tagaaccaat
     7321 tttaacgttt aagttagcag tatcgcccgc ttttggtgtc aggaaggatt gtaactgagc
     7381 actgttacta tcaccagaat ttgtaccagc tgcaatagtt gcactatatg taaaatcacc
     7441 tgtagcgctg ttgtatgtat aatctgtagc tgttggcgat ccaaaaccat ttgcaattgt
     7501 ggcattaact tttgcgttat tagccaaact cgcaattaca tctgcagctg tagatgtttt
     7561 caggcctgcg ctaacagtat aggtaactgt accattagca tcagcagtac ctggagccaa
     7621 gagttgagct gctgccaaat cagatttagt cgctgcagta ttagccacag ccccgccacc
     7681 attcacatta aacccactca gccccagcgt atcagagtca attttcttca gatcaatagt
     7741 gatagtctgg ccgtcattcg caccaacctg aattttcatt gaaccgtctt tcgccagtac
     7801 gttcacgccg ttgaactggg tctggccaga tacgcggtca atttcgtcca gacgggattt
     7861 gatttcgtcc tgaatggagt ccagatccga atcggagtta gtcccggtag aagcctgaac
     7921 cgtcagttca cggatacgct gtaagttgtt gttgatttcg gacagcgcgc cttcggtggt
     7981 ctgtgcaacg gagataccgt cgttggcgtt acgtgcagct tgagtcagac ctttaatgtt
     8041 agaagtaaaa cggttagcaa tcgcctgacc tgcggcgtca tccttcgcgc tgttaatacg
     8101 caagccagaa gacagacgct cgatagaact cgacagcgca gactggttct tgttgatatt
     8161 attttgagtg atcagcgaga ggctgttggt attaatgact tgtgccatga ttcgttatcc
     8221 tatattgcaa gtcgttgatt acgtattggg tttccacccg tcggctcaat cgccgtcaac
     8281 cctgttatcg tctgtcgtaa aacaaccttt agaatttttt tcacaaacag ccattttttg
     8341 ttagtcgccg aaatactctt ttctctgccc cttattcccg ctattaaaaa aaacaattaa
     8401 acgtaaactt tgcgcaattc aggccgataa ccccggtatt cgttttacgt gtcgaaagat
     8461 aaaaggaaat cgcatggcaa gtatttcatc gctgggagtc gggtcaggtc tggatttaag
     8521 ttcaatcctt gatagcctca ccgccgcgca aaaagcgacg ctaaccccta tttcaaatca
     8581 gcaatcgtcg tttaccgcta aacttagcgc ctacggtacg ctgaaaagcg cgctgacgac
     8641 tttccagacc gccaatactg cattgtctaa agccgatctt ttttccgcca ccagcactac
     8701 cagcagcacc accgcgttca gtgccaccac tgcgggtaac gccatcgccg ggaaatacac
     8761 catcagcgtc acccatctgg cgcaggcgca aaccctgacc acgcgcacca ccagagacga
     8821 tacgaaaacg gcgatcgcca ccagcgacag taaactcatc attcaacaag gcggcgacaa
     8881 agatccgatt accattgata tcagcgcggc taactcatcg ttaagcggga tccgtgatgc
     8941 tatcaacaac gcaaaagcgg gcgtaagcgc aagcatcatt aacgtgggta acggtgaata
     9001 tcgtctgtca gtcacatcaa atgacaccgg ccttgataat gcgatgacac tctcggtcag
     9061 cggtgatgat gcgctacaaa gttttatggg ctatgacgcc agtgccagca gcaacggcat
     9121 ggaggtctcg gtcgccgccc agaatgcgca gctgacggtc aacaacgtcg ccattgagaa
     9181 cagcagcaac accatcagcg acgcgctgga aaacatcacc ctgaacctga acgatgtcac
     9241 cacgggcaac cagacgctaa ccatcactca ggacacctcc aaagcgcaaa cggcgattaa
     9301 agactgggtg aatgcctata actcgctaat agataccttc agcagcctga ccaaatacac
     9361 cgccgtagat gcgggagctg atagccagag ttctagcaat ggcgcactgc tcggcgactc
     9421 cacgttgcgg acgattcaga cgcagttgaa atcgatgctg agtaataccg ttagttcttc
     9481 caactataaa acgttggcgc agattggtat cacgaccgat cccagcgatg gcaaactgga
     9541 actggatgcc gacaaactca ccgctgcact gaaaaaagat gccagcggcg taggtgcatt
     9601 gattgttggc gatggtaaaa aaaccggcat cacgaccacc atcggcagca acctgaccag
     9661 ttggctttcg acaacaggca ttattaaagc cgctaccgat ggcgttagta agaccctgaa
     9721 taagttaacc aaagactaca acgccgccag cgatcgcatt gatgcgcagg tcgcgcgcta
     9781 caaagagcaa tttacccaac tggacgtttt aatgacctcg ttaaacagca ccagcagcta
     9841 cttaacgcag cagttcgaaa acaacagtaa ttccaagtaa tcaatattca tcgggagaca
     9901 ggtcatgtac gcggcaaaag gcacccaggc ctatgcacaa attggcgtcg aaagcgccgt
     9961 aatgagcgcc agccagcaac agctggtcac catgctattt gatggagtgc tgagcgcact
    10021 ggttagagcg cgcctgttta tgcaggacaa caatcagcaa ggcaaaggcg tctctttgtc
    10081 aaaagcgatc aacatcattg agaacgggct gcgggtgagt cttgatgaag agagcaaaga
    10141 cgaactaacc caaaacttga ttgctcttta tagctatatg gtcaggcgct tgctgcaagc
    10201 caatttacgc aacgatgtct ccgcagtcga agaagtggaa gcattaatgc gcaatattgc
    10261 cgatgcctgg aaagagtctt tactctcccc ttctttgatt caggacccag actgatgaac
    10321 aatgcaccgc atttgtattt cgcctggcaa caactcgtcg aaaaaagtca gctcatgtta
    10381 cgcctggcga cagaagaaca atgggacgaa ctcatcgcca gcgaaatggc gtatgtgaat
    10441 gcggtgcagg agatcgcaca cctgactgaa gaggttgccc cgtccaccac gatgcaggag
    10501 cagctccgcc cgatgctgcg cctgattctc gacaacgaaa gcaaggtaaa gcagttatta
    10561 cagattcgga tggatgaact ggcgaaactg gtcggtcagt catcggtgca aaaatcggtg
    10621 ttaagtgcct atggcgatca gggtggcttt gtgctggctc cgcaggataa cctcttttga
    10681 atcttaaaga gccaatggct cgcgaataat ctgattacgg ctacgcttct aatgttcccc
    10741 ttgaatggag tcgaagaatg cgtaatccca cgctgttaca atgttttcac tggtattacc
    10801 cggaaggcgg taagctctgg cctgaactgg ccgagcgcgc cgacggtttt aatgatattg
    10861 gtatcaatat ggtctggttg ccgcccgcct ataaaggcgc atcgggcggg tactcggtcg
    10921 gctacgactc ctatgattta tttgatttag gcgagtttga tcagaaaggc agcatcccta
    10981 ctaaatatgg cgataaagca caactgctgg ccgccattga cgccctgaaa cgtaatgaca
    11041 ttgcggtgct gctggacgtg gtagtcaacc acaaaatggg cgcggatgaa aaagaagcta
    11101 ttcgcgtgca acgtgtaaat gctgatgacc gtacgcaaat tgacgaagaa atcattgagt
    11161 gcgaaggctg gacgcgttac accttccccg cgcgtgccgg gcaatactcg cagtttatct
    11221 gggatttcaa atgttttagc ggtatcgacc atatcgaaaa ccctgatgaa gatggcattt
    11281 ttaaaattgt taacgactat accggagaag gctggaacga tcaggttgat gatgaactcg
    11341 gtaatttcga ttatctgatg ggtgagaata tcgattttcg caatcatgcc gtgacggaag
    11401 agattaaata ctgggcgcgc tgggtgatgg aacaaacgca atgcgacggc tttcgtcttg
    11461 atgcggtcaa acatattccg gcatggtttt ataaagagtg gatcgaacac gtacaggaag
    11521 ttgcgccaaa gccgctgttt attgtggctg aatactggtc gcatgaagtc gataaactgc
    11581 aaacgtatat tgatcaggtg gaaggcaaaa ccatgctgtt tgatgcgccg ctgcagatga
    11641 aattccatga agcatcgcgc atgggtcgcg actacgacat gacgcagatc ttcactggga
    11701 cgctggtgga agccgatcct tttcacgccg taaccctcgt tgccaaccac gacacccaac
    11761 cgttgcaagc cctcgaagca ccggtcgaac cgtggtttaa accgctggcg tatgccttaa
    11821 ttttgttgcg ggaaaatggc gttccttcgg tgttctatcc ggacctctac ggtgcacatt
    11881 acgaagatgt cggtggtgac gggcaaacct atccgataga tatgccaata atcgaacagc
    11941 ttgatgagtt aattctcgcc cgtcagcgtt tcgcccacgg tgtacagacg ttatttttcg
    12001 accatccgaa ctgcattgcc tttagccgca gtggcaccga cgaatatccc ggctgcgtgg
    12061 tggtcatgtc gaacggggat gatggcgaaa aaaccattca tctgggagag aattacggca
    12121 ataaaacctg gcgtgatttt ctggggaatc ggcaagagag cgtagtgacc gacgaaaacg
    12181 gcgaagcgac cttcttttgc aacggcggca gcgtcagcgt gtgggttatc gaagaggtga
    12241 tttaaattca tccccggcgg caagccgggg agatttcatt acggcagtgg cgtcggtaac
    12301 ggcgctttat ccagcgcggc ggcacactct gccgtcgggc gatccacacg ctccatcgtc
    12361 ataccatcgt actcaatggt gttaccgtca cgctcaacct cgtacagctc acgtttcacg
    12421 gtaacgttag tgagatcatc agacatcaat gtcaacttac ccggcacggc aatcacccgc
    12481 tgccactgac ggcaatccag ggtatcgccc tctttagtga caatcaggct gcctatcgct
    12541 tccggactga ccagcgcact ttgcggccct ttggtctgcc agtagcccgc cagccagtcc
    12601 ggcgcggggg ttttcacgac attgttatag ttttcgactt cggcacagcc cgccagcaac
    12661 atgagcgcgc ctgcaattgc gagttttttc atcattcgtc ctgcatgaga agaaaagagg
    12721 attgtggcat taaagccctg atgtcgccag cctttccagg ttcagagaga aatttgatct
    12781 aactattatt tttgtgaatt attaccccga aaataaattt gcttgatgta tgattcgcaa
    12841 ctcttgtttc aaaccttctg agttcagagg ctacatacat gtcatggcag caattcaaac
    12901 acgcctggtt gattaaattc tgggccccca tccctgcggt catcgcggcg ggtattctct
    12961 ccacttacta ctttggcatc actggcacct tttgggctgt cacgggtgaa tttacccgct
    13021 ggggcggtca gcttctgcag ctgttcggcg tccatgctga agagtggggt tattttaaaa
    13081 ttatccatct ggaaggatcg ccattaaccc gcatcgacgg catgatgatc ctcggtatgt
    13141 ttggcggctg ctttgctgca gcgctgtggg ccaacaatgt caaactacgc atgccacgca
    13201 gccgtatccg cattatgcag gccattattg gcggcattat cgctggtttt ggcgcgcgtc
    13261 tggcaatggg ctgtaacctg gcggcgttct ttaccggcat tcctcagttc tcgctgcatg
    13321 cctggttctt tgccatcgcc actgccattg gttcatggtt tggcgcgcgc tttaccctgc
    13381 tgcccatctt ccgtattccc gtgaaaatgc agaaagtttc tgccgcctca ccgctgacgc
    13441 aaaaaccgga tcaggcgcgg cgtcgttttc gtctcgggat gctggtcttt ttcggcatgc
    13501 tgggctgggc gctgctcacg gcgatgaacc aaccaaaact ggggctggca atgctgtttg
    13561 gcgtcggctt tggtttactg attgaacgtg cgcaaatctg ctttacttcg gcgttccgcg
    13621 atatgtggat caccggacgt acccatatgg cgaaagcaat cattatcggt atggcggtga
    13681 gtgccatcgg gatcttcagt tacgtacagt taggcgttga acctaaaatc atgtgggcgg
    13741 gaccaaatgc ggtaattggt ggtttacttt ttggttttgg catcgtgctg gcaggcggct
    13801 gcgaaaccgg ctggatgtac cgcgcggtag aaggccaggt gcactactgg tgggtcggtc
    13861 tgggcaacgt gatcggctca acgattctgg cgtattactg ggatgatttc gctccggcgc
    13921 tggccaccga ctgggacaaa atcaacctgc tgaaaacctt tggtccgatg ggtggcctgc
    13981 tggtgacata tttgctgttg tttgcagctc taatgttgat tatcggctgg gaaaaacgct
    14041 tcttccgccg tgcggcaccg cagattgcta aggagatcgc atgaaaaata tcgttcctga
    14101 ttaccgtctt gatatggtgg gtgaaccctg cccttatccg gctgtcgcaa cccttgaggc
    14161 gatgccgcag ttaaaaaaag gggagatcct ggaagtggtg agcgactgtc cgcagtcgat
    14221 caataatatt ccactggatg cgcgtaatca cggctatacg gtgctggata ttcagcaaga
    14281 cgggccgacc attcgttatt taattcagaa gtaatccctc attcccgctg tggcccacag
    14341 cgggaacatc gttaccactc tatcacatag attttacccg ccccctctcc tattcttttc
    14401 agtgtgctct caggaggttt ctatgtgtgg acgctttgcc caatcccaaa cgcgtgaaga
    14461 ttacctcgcg tttctcgcgg aagatattga acgcgatatt ccctacgatc ccgaacctat
    14521 tggcagatac aacgtcgcgc cgggaaccaa agtccttctg ctcagtgaac gtgatgaaca
    14581 ccttcatctg gatccggttt tctggggata tgctcccgga tggtgggata aaccgccgct
    14641 gattaacgcc cgcgtagaaa ctgccgcaac cagtcgcatg tttaaaccgc tctggcaaca
    14701 cggtcgggca atctgttttg ccgatggctg gtttgagtgg aaaaaagaag gcgacaaaaa
    14761 acagccttat tttatctatc gcgctgacgg acaacctgtt tttatggccg cgataggtag
    14821 cacaccgttt gagcgtggtg acgaagccga aggatttttg atcgtcactg cggcggcaga
    14881 tcaaggtctg gtagatattc atgaccgccg cccgctggtt ctgtcgccag aagctgtacg
    14941 ggaatggatg cggcaagagg ttggcggtaa agaagcctca gaaattgctg ccagtggctg
    15001 tgtgccagca aaccagttca cctggcaccc ggtatcgtgc gcggtgggta atgttaaaaa
    15061 ccagggagcg gcgttaattc aacctgtttg ataagcctgg agattattga tcaacaatac
    15121 tgcgtcataa gaaatctcta ttagacaaag atttcagtac ctgttggcat attgcaaaaa
    15181 taacaccaat acggaatcgt catgttcacg attaaaacag atgatctcac ccatccagca
    15241 gtgcaagcat tagtggctta ccatatttcc ggcatgctgc agcagtctcc ccccgaaagt
    15301 agtcatgctt tagatgtgca aaaattacgt aacccgaaag tgacattctg gtcagtatgg
    15361 gaaggcgaac aactcgcagg aattggtgcg ctgaagttgc tggatgataa acatggcgaa
    15421 ctgaaatcaa tgcgtaccgc gccaaattat ttacgtcggg gtgtcgccag tctgatttta
    15481 cgccacattt tgcaggtcgc ccatgacaga tgccttcatc gcctgagctt agaaacgggt
    15541 acacaggctg gatttacggc ctgccatcaa ctttatttga aacatggatt cgttgattgc
    15601 gaaccgtttg ctgattatca acttgatccg cacagtcgat ttttgtcatt gacgctatgc
    15661 gaaaataatg aattgccatg agccagacgc agcacattct tgcattcgac gtgctgcgcc
    15721 tttatttatt atcagccgca aatgtcttgt ccttagacgc tctttccaca tgcgccacaa
    15781 gaaacctcta ttccagtgac acaattatgc ctgattattt ccctagcccg gaatatgaat
    15841 aaaaa
//
LOCUS       DAZUCC010000117        15756 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZUCC010000117 DAZUCC010000000
VERSION     DAZUCC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.117"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            315..641
                     /locus_tag="ACHY09_003088"
     CDS             315..641
                     /locus_tag="ACHY09_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFS3886032.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(773..973)
                     /locus_tag="ACHY09_003089"
     CDS             complement(773..973)
                     /locus_tag="ACHY09_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS3886033.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALQHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            1015..1380
                     /locus_tag="ACHY09_003090"
     CDS             1015..1380
                     /locus_tag="ACHY09_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS3886034.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            1663..2220
                     /locus_tag="ACHY09_003091"
     CDS             1663..2220
                     /locus_tag="ACHY09_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS3886035.1"
                     /translation="MLTTQKRKFALALMSGKNRTAAAIEAGYSEKSARCKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQACHREVPPVEKKNQVSVPPDDGQSFSPLSG
                     IDYMEDGLPDPVKAMGRILVENISIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGDAA
                     KKAANRFAVPPPPRLVVNNDNEGNG"
     gene            2217..3878
                     /locus_tag="ACHY09_003092"
     CDS             2217..3878
                     /locus_tag="ACHY09_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFS3886036.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVASGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            3942..5879
                     /locus_tag="ACHY09_003093"
     CDS             3942..5879
                     /locus_tag="ACHY09_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS3886037.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGVHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            5924..6145
                     /locus_tag="ACHY09_003094"
     CDS             5924..6145
                     /locus_tag="ACHY09_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886038.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            6091..8675
                     /locus_tag="ACHY09_003095"
                     /pseudo
     CDS             6091..8675
                     /locus_tag="ACHY09_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            8672..8998
                     /locus_tag="ACHY09_003096"
     CDS             8672..8998
                     /locus_tag="ACHY09_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS3886039.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            9008..9358
                     /locus_tag="ACHY09_003097"
     CDS             9008..9358
                     /locus_tag="ACHY09_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS3886040.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            9355..9801
                     /locus_tag="ACHY09_003098"
     CDS             9355..9801
                     /locus_tag="ACHY09_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS3886041.1"
                     /translation="MIETLLDFSGLEEISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VASRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            9798..10142
                     /locus_tag="ACHY09_003099"
     CDS             9798..10142
                     /locus_tag="ACHY09_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFS3886042.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            10208..10924
                     /locus_tag="ACHY09_003100"
     CDS             10208..10924
                     /locus_tag="ACHY09_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFS3886043.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            10939..11313
                     /locus_tag="ACHY09_003101"
     CDS             10939..11313
                     /locus_tag="ACHY09_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFS3886044.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            11409..11618
                     /locus_tag="ACHY09_003102"
     CDS             11409..11618
                     /locus_tag="ACHY09_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFS3886045.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEADPLAALEDALLFASEN"
     gene            11682..12095
                     /locus_tag="ACHY09_003103"
     CDS             11682..12095
                     /locus_tag="ACHY09_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886046.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLEKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            12159..15401
                     /locus_tag="ACHY09_003104"
     CDS             12159..15401
                     /locus_tag="ACHY09_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS3886047.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAITQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEERTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEELAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSETEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREEALKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            15394..15735
                     /locus_tag="ACHY09_003105"
     CDS             15394..15735
                     /locus_tag="ACHY09_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3886048.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVR"
BASE COUNT         3582 a         3735 c         5039 g         3400 t
ORIGIN      
        1 gccgtattcc cgtattaaca gagaccgcag cccgacaggg agactcctct gcgcgagtgt
       61 gcggggataa tcaaaaacga tacacaccgg ggtttaccgc gttaacggag cgcggcgttg
      121 tcccctcatg gtcgctggtc cggtgcgatg gtggaagaaa ccggacgatg tgttacctcg
      181 caagctctgt tatgtcatgt gtctgatttg tgatttaagt cggataattg tcgttgccat
      241 taagcagagg attgatgacc gacagggtgg cattgttaga ataagactta ttcttatctg
      301 tgccgggaat gaaaatgaaa agaaatcttc cgttaattat tttgttgtct tctctggtta
      361 tgggctgtac gcaacataaa acagatatgc cccgacagtt ggttaaggca ttaccacaat
      421 atccggccta tgcagcggca aattatataa agggacgggt tgatgtgaag tttgatattg
      481 gtgctgatgg tactgtcacc cgaattgagt ttatccgttc agagccgcac catctgtttg
      541 atgagcaggt tgtaaaggcg atggcaaaat ggcgatttga gaaggacagg ccgcgtaaag
      601 gcgtgaagaa aacgtttatc tttagtcctt ctgcaccctg attatttcat cagaaattaa
      661 ttatcactct gttgttattc tgtacatccc ggctgggtaa gtcttgttcc gccgggtatg
      721 aagatgaaat attgttggag gacagtgggt acctgctcct gtaaccgaac gttcatttct
      781 cgttatttgt catgctggcc ggacgcagat gcgttgcatc tgttgccagc cttctcctgc
      841 aggcttcaat aacccacgct gaaaagttac cggacccttt atgctgaagg gcgatgttga
      901 tctgttcaat catgtgattg gggaaacgga tattgcgggt tgtggttctg cgggtccggt
      961 ttttcgatga catatttatt tcctttactg attgccatat gacggggatt ttacatggct
     1021 gagcttcgta cactccagag cagaatcaaa acactgaata cccgacgggt gaatattctg
     1081 aagggtgaac agcgtcgtgt cagtggcagt gcacgtgttt ccctcaagcg tcatatctgg
     1141 ctcagggatg ccgggcagtg ctgtctctgt ggtcgtgtgg ttgacctctg tgacagtgaa
     1201 ctcgatcacc gaattgcact tcagttcggt ggtggtaatg aggagacgaa tctctggacg
     1261 ctctgtaccg aatgccatcg acaaaagtct gctcgtgaag cggcgggtgg tatgccggac
     1321 ccgacgctgc cggaggtgtc cggaggtagt ggcagagcgg acgacattat cggactgtaa
     1381 cccgaccggg gggggatcat ccggcgtaaa aaacgatcgc tccggacacc gcgccccctc
     1441 tcacgcagag aaaaaattcc ccttcagaag ttataaacat gtttataacc tactgatgga
     1501 tttttaccgt ttttttcttt ttattcataa cgttgtggga ggtaaatgtt tatacctttt
     1561 tttcgcaaaa tgtttacagg gcagcggtaa acattttccc tgccgcctcc attcttaccg
     1621 ggaggattgc tgacgttttt ctgtttttcc ccggaggaaa gaatgctgac gacgcaaaaa
     1681 agaaaatttg cgctggcgct catgtcgggc aaaaacagaa cggctgctgc tattgaggct
     1741 ggttactcgg aaaagtccgc cagatgtaaa ggctcacaac tggcaaaaga tccggaagtg
     1801 ctcgcgttta tagcccgtaa gcagggcgag acggtggagg tggatgaggt tcctgtttac
     1861 cggcagaaaa agacgcaggc gtgccaccgg gaagttcctc cggtggaaaa gaaaaaccag
     1921 gtttcagtgc caccggatga cgggcaaagt ttctctccac tatccggtat tgattacatg
     1981 gaggacggcc ttcctgatcc tgtgaaagcc atgggtcgga tcctggtgga aaacatcagc
     2041 attgatccga aactggctct ggatgcagcc tggcgactgg cgcagttcac gcaccacaaa
     2101 aaaggggatg ccggtaaaaa atcggcaaaa ggtgacgcgg cgaaaaaagc ggctaaccgt
     2161 tttgcggtgc caccaccacc ccgcctggtg gtgaataatg ataatgaggg caacggatga
     2221 tacctgtgtg gagcacggcc tgcccggact gggcagagcg tctgaaaaag gggctgtcga
     2281 ttattccggc tccgatttac ccggaccagg ccgcacatgc cctggcgatt tttaaacaac
     2341 tgcggattgt ggatgcaccg ggcagcccga cgttcgggga gtcctgtgca ccgtgggtgt
     2401 ttgatctggt ggctgccctg tttggctcct acgatgcgca gaccggtgta cgccatataa
     2461 aggaagtgtt cattctgata cccaaaaaaa acagcaagtc cacgctggct gccgggatca
     2521 tgatgacggc gctgttactg aactggcggc aggcggcggg ctacaccatt ctggccccga
     2581 ccgtggaggt ggcggctaac gccttcaacc ctgccaggga tatggtacga cgggacgatg
     2641 atctggatga cctctgtcag gtgcagacac atatccggac catcacccat cgggtgacgg
     2701 acaccaccct taaggtggtg gctgccgatc cgaatacggt gtccggtatc aagtccgtgg
     2761 ggacgctgat tgatgaactg tggttatttg gcaagcagta caaagcggag gacatgttac
     2821 gtgaagccat cggcggcctt gcctcccgcc cggaagggtt tgtggtgtac acaaccaccc
     2881 agtcgaatga gccgcccgcc ggggtgttca gacagaaact gcagtacgcc cgggatgtgc
     2941 gcgacggcaa aattaatgat ccgcactttc tgccggtgat atttgagcat ccgccggaga
     3001 tggtggagcg cggtgagcac ctgctgatgg aaaacctcgc catggttaac ccgaatctcg
     3061 gttattcggt ggatgaggct tttctgtacc gggagtaccg taaagcccgg gaggctggtg
     3121 aggaggcatt tcgtggcttc atgtcaaaac acgccaatgt ggaaattggt cttgccctgc
     3181 gttctgaccg ctgggcgggt gcggatttct gggagcagca gggcaggcgc gtcagcctgg
     3241 acgatatcct gcagcgcgct gatgtggtga cggtggggat tgacggcggg ggcctggatg
     3301 atctgctggg aatgtacgtg attggtcgtg acagggaaac ccgcgaatgg ctgggctggg
     3361 gccatgcctg ggcgcatgaa accgcggtgg tcagacggaa gagtgaggca tcccggtttc
     3421 aggattttgt ggccagtgga gacatgacga ttgtccgtcg ggtcggggat gacacggcgg
     3481 aagtggcgga gtatgtgcgt cgtattcatg aggctgagtt actggaccat atcggtattg
     3541 acccgtcagg tgtggggcag attctggatt cactggcaga agccgggatc cccgacggaa
     3601 ttgttgtggg gataagccag ggctggaagc tgggcggggc catcaaaacc accgagcgca
     3661 aactggctga gggagtgctg gtgcatggtg gtcagccact gatggcctgg tgcgttggca
     3721 atgcccgggt ggagcctaaa ggtaacgcca tccttattac caaacaggcc agcggacggg
     3781 ggaaaattga cccgctgatg gcgctgttca atgcggtatc cctgatgtcc ctgaatccgg
     3841 agcccaaaaa gaaagaatat gcggtttttt tcatataacc ctgttcaccc tgtaaccatc
     3901 atggaccgct gcggcggttt ttttattttc aggaggctga tgtgactctt aaacgggcct
     3961 gctccctgct gacggtgaaa tccttcagtg aggatgagcg ggtgatcacc gggattgcgt
     4021 caacgccttc tccggatcgg gatggtgaca tcctggagcc ggagggcgcg gagtttggca
     4081 gtgcgatccc gtttctctgg cagcatgacc attcccgccc ggtggggcag tgtacggtgc
     4141 gccgggtcag cgaagggctg gaaatcacgg caacactggt gaaacccgta ccggatatgc
     4201 cgtcgcaact ggctgcccgg ctggatgagg tctgggcggc cattaagacc gggctggtca
     4261 gggggctgtc cgtgggcttc cgtcctcatg aatacacctt tctggacgga ggcggagtgc
     4321 attttctgcg ctgggagctg atggaggtgt ctgccgtcac cgtgcccgcg aatgcggaat
     4381 gcactatccg gaccattaaa tcttacgacc gcccgttttc tgccgcgtcc ggcaaccgga
     4441 aaccggtggt gaaaatcgca tcttctgccg gcgctgcggc acagtcaaca accgtttttc
     4501 ataaggaaaa gaccataatg aatattggcg aacagattaa aagttttgaa aacaagcgtg
     4561 cagcgctggc agcctccctt gaggaggtca tgaccaaagc cgcagaggaa gggcgcacgc
     4621 tggatgtgga ggaggaagag cattacgaca acaccgcagc ggaaatccgt caggtggatg
     4681 cgcacctgaa gcgcctgcgt gaactggaag ccggtaaggc cgccacggcg cagccggtga
     4741 aacaggccgg taacgggaat gtggccgcgg tggcttctgc gccggtgatc cgtgtggagc
     4801 agaaactgga taaggggatt ggtttcgcac gttttgccaa atcgctggct gcggctaaag
     4861 gtgtccgctc tgaagccctg gaagtggccc gtcgtcagta tccggatgac agtcgtctgc
     4921 atcatgtcct gaaatcggca gtgggcgcgg ggaccaccac ggatccgcag tgggcaggca
     4981 gcctgtctga atatcaggaa tacgcacagg actttattga ttacctgcgt cctcagacca
     5041 ttatcgggcg atttggtcag ggcgggatcc ctgcacttcg tcaggtgccg ttcaatatcc
     5101 gtgtgcacgc ccaggtgtcc ggcggtgctg ccggctgggt gggtgagggt aaggcaaaac
     5161 ccctgacgaa gtttgatttt gaatccatca ccttcagtca tgcgaaggtg tcggccattg
     5221 cggtactgac ggaagaattg atccgttttt ccagtccggc tgctgatgca ctggtccgta
     5281 atgcgctggc ggaagcggtg gtggcgcgtc tggatacaga ctttgtggac ccgaaaaaag
     5341 cggcggtggc agatgtctcc ccggcgtcca tcacccatga tgtgaagggc acggcatcaa
     5401 gcggtaaccc ggatgcggat gcagaggctg cgtttggaca gtttgtggca gcaaacctgc
     5461 aacccaccgg tgcggtctgg ctgatgtcca gcaccaatgc cctggcactg tccatgcgta
     5521 aaaatgcgct gggtcagaaa gaatacccgg acatgaccct gctgggtggc tccttccagg
     5581 ggctgccggt gattgtctcc cagtacgtgg gtgaccagct ggtgctggtg aatgccccgg
     5641 atatttatct ggcggatgac ggcggcgtgg cagtggatat gtcccgcgag gcatcactgg
     5701 aaatgcagtc tgagccgacc ggcgacagta ccacgccgtc gccggtggag ctggtttcca
     5761 tgttccagac aggcagtgtg gccatccgtg cggagcgctg gatcaactgg cgtcgtcgcc
     5821 gtaccgcggc ggtggcggtg atcaccggtg tgaactacgg cagtgcgtcc ggcggctgag
     5881 tctgataagg aggacgggag gcgtgcgcct cccgtaacag gttatggcaa agatccgata
     5941 cctgcagggc acgcatgatg cccgggccgg ggatatccgg gatgtggcac agccgtgtgc
     6001 ggaggtgctg gttcgcctgg gaaaggcaga gtacatcacg gtgcgacgtc cggcaggtca
     6061 gaaaaagaaa cgtgatgcgg agcatggcga atgtggaacc ttttacggcg aacccgaaaa
     6121 aaccagaaat caggacgtga cgtaagagag gcgggctgga ccagcctgtt tcaggcggtg
     6181 gctgagccct tttccggcgc ctggcagcag ggcgtgaaag ccgatcctga agccgtcctc
     6241 tcctttcatg cggtgtttgc atgtatttcg ctgatatccc aggatatcgc caaaatgcgg
     6301 ctgcgtctta tgcagacgga tgcgcagggg atacgcaggg aaacgcgccg gggagatatt
     6361 gcccgcctct gtcgtcgtcc caacgcccag cagaaccgca tccagttttt tgaactgtgg
     6421 ctgaacgcca aactgcgtca cggcaatacg gtggtgctga aaatccgtaa tgcccggggg
     6481 cagatcaaag aactgcgtat tctggactgg aaccgggttg aacctctggt ggcggatgac
     6541 ggcgaggtgt tctaccgcat cacgccggac cggaactgtg ggatcactga ggcggtgacg
     6601 gtgcctgccc gggaagtgat ccacgatcgg tttaactgtt tttttcatcc gcttataggg
     6661 ttgccgccgg tgtatgccgc cgggctggcg gccacgcagg ggcatcatat tcaggaaaat
     6721 tcgacgtctt ttttcagaaa tggcggcagg ccgtccgggg tgattgagat ccccggcagt
     6781 attacggaag aaaatgcgaa aaaactgaag agcaactggg acagcgggta tacaggcgaa
     6841 aatgcgggga aaacggccat tctgagtaac ggggcaaaat acaaccccac gacgttttca
     6901 ccggtggatg cgcagacggt ggaacaactg aagatgaccg ctgaaattgt ctgttcggtg
     6961 ttccgtgtcc cggcctacaa gattggcgtg ggacaaccgc cttccagtga caacgtggag
     7021 gcgctggagc agcagtatta ttcccagtgc ctgcagacac tgattgagtc cattgagctg
     7081 ttactggatg aggcgctgga aacgggggaa aacgagagta cggaatttga tgtcaccacg
     7141 ctgctgagaa tggacagtga gcggcgcatg aaaacgctgg gggatgcggt gaaaaatacg
     7201 cttctcacgc ccaatgaagc ccgtaaacgg gagaacctgc cgcccctggc cggcggtgat
     7261 gcactgtatc ttcagcagca gaactacagt ctggaagcgt tgtcccgccg tgatgcccgt
     7321 gaagatccgt tcgcgtcgtc cggtaaaaca gcttccgtgc ctcaggcggt cgctgcatct
     7381 gacggtaata aggcaatcac tgaaacagag catgacgcgg tgaaggcgat gttcaggggg
     7441 attctgaaaa aatgaatgaa cgtgaactgt ccattatccg tgcgctgggt gaagaatttt
     7501 ccgcggtgct ggcggattta cagcgcacat ttgaggagaa aatagccgcg caggcacaaa
     7561 cgtttgaaga aaaactggct tccctgtctg tggtattaca gaagtgcgtg acgggcgatg
     7621 atgtgcgtcc gatgcttgag cagatggtga aggaggcggt aagccatatc cctgttccgc
     7681 gagatggtcg tgactacgat ccggaagtac tgcagaaggc ggtgaatgat gcggtcgcaa
     7741 atattccgca gccggcggac ggtaaaagta tcaccccgga tgatgtgcgt ccgatgcttg
     7801 aacagatggt gaaagaggca gtgagccata ttcctgttcc gcgtgatggt cgtgactacg
     7861 atccggaagt actgcagaag gcggtgaatg atgcggtcgc aaatattccg cagctggcag
     7921 acggtaaaag cctcaccccg gatgatgtgc gtccgatgct tgagcagatg gtgaaggagg
     7981 cggtaagcca tattcctgtt ccgcgtgatg gtcgtgacta tgatccggaa gtactgcaga
     8041 aggcggtgaa tgatgcggtc gcaaatattc cgcagccggc agacggtaaa agtatcaccc
     8101 cggatgatgt gcgtccgatg cttgagcaga tggtgaagga ggcggtaagc catattcctg
     8161 ttccgcgtga tggtcgtgac tatgatccgg atgttctgca gaaggcggtg aatgatgcgg
     8221 tgagtgccct gccggctccg caggacgggc gtgatgccac ggcactggaa atactccctg
     8281 ccattgacga tcaaaaatcc tttccccggg gcacgtatgc cacacaccag ggcggactct
     8341 ggcgggcgta tgaaaaaacg cacgggatgc ggggatggga atgcctggtt gacggggtgg
     8401 cggatattga cgtcagcatg acgggtgaac ggttgttctc tgtggtggtc cggcagagca
     8461 gtggccagcg tacggaaaaa acattttccc tgccggtgat gctctaccgc ggtgtgttca
     8521 gagccggtga aacctaccac cccggcgata cggtgacgtg ggggggctcg ctgtggcact
     8581 gcaacagtat gaccggtgat aaacccggag aagctcattc atcaggctgg accctggctg
     8641 caaaacgtgg gcgggatgct ggaggcggaa aatgacggca ttactgacac tggaagagat
     8701 caaggcacat ctgcgtgtcg accatgacgc ggatgatgac atgctgatgg acaaggttcg
     8761 tcaggctacc gccgtgctgc tggcctacat tcagggcagc cgggataagg tgattcgtga
     8821 ggacggtgaa ctgatcccgg gcgaggcatt aacccggatg aagggggctg ccatgcgact
     8881 gaccgggatg ctgtaccgga atccggatct tgcggagcgg gaagaactgc ttcaggggga
     8941 gctgccgttt tctgtttccg tgctgattta cgatttgcgt tgtccgacgg tgttatgagg
     9001 agggggaatg gcaatatctg caggtcgtct gacacagatg ataagtgttc tgaacccggt
     9061 gttaacccgt aatgctgccg gagaaatgac ggaagaatgg gtgtcatgcg ggaaaattca
     9121 tgcggatatc cgtggcagga gcagccggga gcggatgcag tccggtgcgg aaatggcgca
     9181 ggcggaaatc cgcatctggg tgcgcggtca gtccggtcgg gaaatcacgg cagcgtcacg
     9241 acttcatgtg ctgagtggtc catggcgtga ccggatcctg aacgttgtcg ggctgcccgt
     9301 gccggatgcg accggcgggc gtctggaaat tctctgtcgg ctgggagggg aaaaatgatc
     9361 gaaaccctgc tggatttttc ggggctggag gagatcagcc gcgatttgca gcttctgagt
     9421 ggtgcggaaa ataaccgggt gctgcgtgag gcaacccgtg cgggtgcgaa tgtgctgaaa
     9481 gaagaagtgg cgtcacgggc accggtacgc aggggaaaac tgcgccgcaa tgtggtggtc
     9541 ctttcccggc gctcccgcga tggcgggatg gaatccggtg tccatatccg tggtgttaat
     9601 ccggacaccg gtaacagcga taacaccatg aaggcggata acccgcgcaa tgctttctac
     9661 tggcggtttg tggaaatggg gaccgtgaat atgccaccgc acccgtttgt gcgcccggcg
     9721 tttgatgtgc gcagtgaaca ggcagcgcag gtggcgattg ctcggatgaa ccgggccatt
     9781 gatgaggtac tgagacgatg acggaggcgg atttgtatcc tcatctggcg catcttgccg
     9841 gcgggcaggt gtacccgtat gtggtccccc tgctggatgg caggccgtcg gtggcgcttc
     9901 cgtgggtggt tttcagcctg atttcatcgg tgtctgcgga cgtgatgggc gggcaggcgg
     9961 agtcctcagt ggcggtgcag atagacgttt atgccgggac tgtgacgcag gcgcgtcaga
    10021 tacgtcagga cgcccgtgaa gccataatgc tgctggcccc gggatccgtc agtgaaatgc
    10081 aggactatat tccggaaaac cgctgttacc gtgcaaccct ggagtttcag gtcacggtgt
    10141 gactttttct tttttctaca aaacccatac cccgccgcgt gcgggttttt tattatcagg
    10201 aggcagaatg tctgctttgt atgaacgctc acagctgacg caggtgatga tttcatctgc
    10261 cccggcgact gctgaaacta tggataaggc ggaatatctg cgcctggact gcaccatcaa
    10321 ggaagtccag ttcaccgccg gtcagaaaca ggatattgat gtgaccacgc tctgctccac
    10381 agagcaggag aatatcaatg gtctgggggc gtcgtccgag atttccatgt cgggtaattt
    10441 ttatctgaat caggcccaga acgccctgcg tgatgcttat gacaatgacg cgttgtatgc
    10501 gtttaaggtg cagtttccgt ccggtaaggg ctttaaattc ctggcggaag tgcgccagca
    10561 cacctggtca tccggtacca acggcgtggt agctgcaacg ttctcactgc gtctgaaagg
    10621 caaaccggtg tcctttgtgg taccgctggc gtttgtgaaa aatctggata agacacttac
    10681 cgtgaatacc ggtgcgctgc tgacaatgtc agtcagtgcc aacgggggaa cgccgccgta
    10741 taaatacgcc tggaagaagg atggtcagcc ggttgacggg cagacgacag acaccttcag
    10801 taagccaggt gcgcagtccg ctgatgcggg gaaatatacc tgcgtggtga ctgattcggc
    10861 agagaaagca cagagtgtga cgtctgttga atgtacagtg acagtgagcg cagccgccgg
    10921 ataaggggat gggtcatcat gaaaaaggat ctgaaaacgc tggcgctggc cagactgtca
    10981 gggtttcgtc ataaaacggt gaaggtgccg gaatggggta atgtcagcgt ggtgctgcgg
    11041 gagccttcgg cagaggcctg gtatctgtgg caggacgtgc tcaatggtga tggagaggat
    11101 gacgataccc tgtcggtggt ggcgaaaacc cgccgtaacc tggaagcgga tgtgacgctg
    11161 ttctgcgatg tcctgtgtga tacggacctg cagcgggtgt tcactccgga cgaccgtgag
    11221 caggtgctgg ccgtctatgg tccggtacat gcccgcttgc tgcgtcaggc actggaactg
    11281 atcgctgatg cagagtcggc cagaaaaaag tagcccgccc ggaaattcgc tttctgatgc
    11341 gacttgcgct ccgtctgggg cgcaccttat ccgaactgcg ccacagcctg agtgcgagcg
    11401 aggcgatgat gtggatggag ttcgacaggg tatccccgct gggtgatgag cgcggggata
    11461 tccgtaatgc acagatcgtg aaagcggttt tcggggcaca ggggatgaat gttgcactga
    11521 aggacgccat gctctgctgg ggcgaggatg aggataagcc ggaggcagat cctttggccg
    11581 ctctggagga tgctctgtta tttgcttcgg aaaattaatt ttaactttgc agatctttat
    11641 tacagtattt tcggggaaaa ttaactgaac ggagccattt tatgaagaaa ttcatgctgt
    11701 tgattttctc tgtcagcttt tttctgacgg ggtgtgcaac cattgtcgga gataaaacgc
    11761 agactgtaca ggtaaacagt aatccgtctg gtgcggattt tattattaaa gatgagtcag
    11821 ggaaaacaat ttcttcgggc aaaacaccgc agaatgtaac acttgaaaaa tctgacggca
    11881 gttacttcgg gaaaaagagt tatcagatca cattcagtaa agagaattat cagcccgtaa
    11941 cgctgccgat caaggcatcg gctaatggat ggtacattgg tggtaacttt gtctttggtg
    12001 gtattgttgg ctggttgctt gtggacccat tcaatggcgg tatgtataca ctgagtccgg
    12061 aaagtctgaa tcccgggctg gtacccaaaa cttaatcaat aatctgctga tgaataaaac
    12121 ctgctgcggc aggttttttt tcgcccggag aaaggtgaat ggcgacgtta cgtgaactga
    12181 ttatcaaaat ttcggcaaat tcacagtcat tccagtcgga gatccagcgg gcgtcccgta
    12241 tgggcagtga atattaccgg accctgcaga atggcggacg tcaggctgct gcggcagcca
    12301 gggagcagcg ccgggctctg gctgagctga acagccagtt gacggaaatc cgcgcttcgg
    12361 ctgtcggaat gaccggtgcg tttgctggtg cctttgccac cggacacctg atttcgctgg
    12421 cggatgaatg gagctccgtg aatgcccgtc tgaaacaggc gtcgcagtca tcggatgaat
    12481 tttcgtcatc acagaaagtg ctgatggata tcagccagcg gacaggcacc gcattttcgg
    12541 ataatgcggc cctgtttgcc cgttcggctg cctcgatgcg tgaatatggt tacagtgctg
    12601 atgatgtgct gaaggtgacg gaggccattt cgacagggct gaaaatttcc ggtgccagtg
    12661 cggcagaggc gggttcggtg atcacccagt tcagccaggc gctggcacag ggggtgttgc
    12721 gcggcgagga atttaattcg gtcaatgaaa gtggggaccg gatcattcgc gcactggctg
    12781 caggcatggg cgtggcccgt aaggatctga aggcgatggc ggacgatggt caactgacgg
    12841 cggataaagt cgttcccgcg ttaatcagcc agctggggat attgcgtgat gaatatgcag
    12901 ccatgccgga aacggtgtct gacgggatca cgaaggtgga aaacgccttt atggcctggg
    12961 tgggcggcgc gaatgaggcc agcggagcga cgaaaacgct ctccggcgca ctgaacggtg
    13021 tggccggaaa tattgatacc gtggcaacag ctgcgggtgt gctggttgct gtcggggtgg
    13081 cccggtactt tggtaatctg gcttccggag cgatgtctgc cacggcagga cttgtgaccg
    13141 ctgcacgtaa tgaagtggca ctggctgagg cccagttaag gggaacgcag attgccacgg
    13201 cgcgggcaag ggcagccgtg taccgggctc agcaggctgt ggcggcagcc cgcgggactg
    13261 agatgcagat tgcggcagag gcccgtctgg cgatcacaca ggaacgcctg aacagaaata
    13321 ttgctgccag aaccgccgcc cagaatgcgc tgaacagtac aacggcggtg ggctcacgtc
    13381 tgatgagcgg tgcgctggga ctggttggag gcgtacccgg actggtgatg ctgggagctg
    13441 ccgcatggta cacgctgtac cagaatcagg aacaggccag ggagtctgcg cgccagtatg
    13501 cactgacgat tgatgaaatc gcgcataaaa cgccatcaat gtctctgcct gaagcctcag
    13561 ataatgaaga gcgaacacgg gaggcgctga cagagcagaa ccggctgatt gatgagcagg
    13621 ccagccgggt gaaatccctg caggaaaaaa tcgccgggta tcagtatgtg ctggctaatc
    13681 cgggctggac gaccggtaac ggcttcatga taaaccatct gacatcggtg aaaactgtaa
    13741 cggaagagct tgctcaggca acagagcagt ttgctgttga gcagtcccgt ctggcacaga
    13801 tgcaggaaaa agcgcagtcc attcaggatg tgcttgccgg actggaagag cgccgtgttg
    13861 tgttaattcg tcagcaggca gcagagcaga ataaagcgta tcagtcactg ctggtcatga
    13921 acggtcagca tacggaattc aaccgcctgc tggggctggg taatgaactg ctgcaacagc
    13981 gtcagggact ggtgaatgta ccgctgcgat tgccacaggc cactctggat gataaacagc
    14041 agagcgccct gaataacaca gagcgtcaac tggccctgtc ccggctgaaa ggggaagaaa
    14101 aagagcgtgc ccggctgggg tatgcggcgg atgaccttgg tctggtgggg gatacgtatc
    14161 aggaggcgag gcagcgttac atccgtaatt cgatggaagc ctggcgcaat aatgaggcga
    14221 ataaacccaa atcccgggcc ggaaaatctg agacggaaaa agcggaagac agtttttccc
    14281 gtctgctgaa gcagcagaag gaacaactgg cactggcagg gaagaataca gaactggcga
    14341 agctgaagta ccagacctcg cagggagagc tgaaaaccct gacggagata cagaagcagg
    14401 agttgctgcg caatgctgcc ctgattgacc agaagaaaat ccgggagcag ttacgggccc
    14461 gggaggaggc tctgaaaaat gataatgcag acgaaagggc atcaaatgac gctgagctgc
    14521 tggggtacgg gcagggtgag cgggtccgtg aacggatgcg ggagctgcag cagattcgtg
    14581 acggctaccg ccagaaggat gcggacctgc agtctcagta tcagacaggg gatatcagtg
    14641 aggattttta cagacaggcg ctggcgcaga atgcgcagta tctgagtgaa cgccttaaag
    14701 accaggaggc tttttatgcc gaatcggatg cgcagcgtgc tgactggcag aaagggctgc
    14761 aggaagggct aagtaactgg gtggacagcg catcagatta cgcttcacag gcagcacagc
    14821 ttgcgacaga cggtatctca gggatggtga ataacatcac ggagatgctg aacggaaata
    14881 aagtggaatg gcgcagctgg gctgcatcaa tcctgcagga aatatcaaaa gttcttatga
    14941 atgcggccat tgtcaacggc attaagatgg ccgcaaacag tatgtccggt gcaggaggat
    15001 ttttcggcag tataggcaac tggctgggtg gcgcggtggc caatgcaaaa ggcggcgttt
    15061 atacctcggc aaacctgagt gcatacagta acagtattgt ggacacgccc acgtactttg
    15121 cctttgcaaa aggggcaggg ctgatggggg aagccggacc tgaagccatc atgcccctga
    15181 cccgggcagc ggatggttca ctcggcgtgc gtgcggtggg cagtatgaac ggcagtgcgg
    15241 gtctggtgta ttccccggtg taccacattg ccattcagaa tgacggcgct aacgggcaga
    15301 tagggccgga agcggcgggc acccttgtgc aactgattga ccagcgggta caggcggtga
    15361 tgttatccat gcgtcgtgac ggaggaatgc tgagtggatg agattaagac ccttcactgg
    15421 tgtccccggg aagggatgca ggtgacggag aaaccgtcgg tggtgacggt gaagtttggc
    15481 gacggttatc agcaacgtcg tccggcagga ctgaatgcgc aactgaagac ctttcaggtg
    15541 gtttttcggg tgacaacgga tgctgagcgg gaggcactgt ccgcgtttct gtcatggcat
    15601 ggtggttacc gggctttttt gtggaagccc ccgaaacata accggacggt cagggtggtg
    15661 tgccgggagt ggagtattac ggataacgcc cggtacagtg atttcagttg cacgatagag
    15721 caggtagtca gatgagaatt atcctataaa tcactc
//
LOCUS       DAZUCC010000118        15432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZUCC010000118 DAZUCC010000000
VERSION     DAZUCC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.118"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..1642
                     /gene="nadB"
                     /locus_tag="ACHY09_003106"
     CDS             20..1642
                     /gene="nadB"
                     /locus_tag="ACHY09_003106"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFS3886049.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDDRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRCMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1627..2364)
                     /gene="trmN"
                     /locus_tag="ACHY09_003107"
     CDS             complement(1627..2364)
                     /gene="trmN"
                     /locus_tag="ACHY09_003107"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFS3886050.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            2496..3830
                     /gene="srmB"
                     /locus_tag="ACHY09_003108"
     CDS             2496..3830
                     /gene="srmB"
                     /locus_tag="ACHY09_003108"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFS3886051.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3863..4744)
                     /gene="yfiE"
                     /locus_tag="ACHY09_003109"
     CDS             complement(3863..4744)
                     /gene="yfiE"
                     /locus_tag="ACHY09_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3886052.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            4847..5434
                     /gene="eamB"
                     /locus_tag="ACHY09_003110"
     CDS             4847..5434
                     /gene="eamB"
                     /locus_tag="ACHY09_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFS3886053.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWIIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(5490..5873)
                     /gene="grcA"
                     /locus_tag="ACHY09_003111"
     CDS             complement(5490..5873)
                     /gene="grcA"
                     /locus_tag="ACHY09_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFS3886054.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            6178..6867
                     /gene="ung"
                     /locus_tag="ACHY09_003112"
     CDS             6178..6867
                     /gene="ung"
                     /locus_tag="ACHY09_003112"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS3886055.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREDVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            complement(6915..7952)
                     /gene="yfiF"
                     /locus_tag="ACHY09_003113"
     CDS             complement(6915..7952)
                     /gene="yfiF"
                     /locus_tag="ACHY09_003113"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFS3886056.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVLQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            8159..8578
                     /gene="trxC"
                     /locus_tag="ACHY09_003114"
     CDS             8159..8578
                     /gene="trxC"
                     /locus_tag="ACHY09_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFS3886057.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            8647..9345
                     /gene="tapT"
                     /locus_tag="ACHY09_003115"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             8647..9345
                     /gene="tapT"
                     /locus_tag="ACHY09_003115"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFS3886058.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPETLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTEAATGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            9377..12037
                     /gene="pat"
                     /locus_tag="ACHY09_003116"
     CDS             9377..12037
                     /gene="pat"
                     /locus_tag="ACHY09_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFS3886059.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKSNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            12151..13506
                     /gene="pssA"
                     /locus_tag="ACHY09_003117"
     CDS             12151..13506
                     /gene="pssA"
                     /locus_tag="ACHY09_003117"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFS3886060.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            13550..13873
                     /gene="yfiM"
                     /locus_tag="ACHY09_003118"
     CDS             13550..13873
                     /gene="yfiM"
                     /locus_tag="ACHY09_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFS3886061.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(13870..15168)
                     /gene="kgtP"
                     /locus_tag="ACHY09_003119"
     CDS             complement(13870..15168)
                     /gene="kgtP"
                     /locus_tag="ACHY09_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFS3886062.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
BASE COUNT         3775 a         4026 c         3918 g         3713 t
ORIGIN      
        1 agtaaattaa acaaagaaaa tgaatactct ccctgaacat tcatgtgacg tgttgattat
       61 cggtagcggc gcagctggac tttcactggc gctacgcctg gctgaccagc atcaggtcat
      121 cgttctaagt aaaggcccgg taacggaagg ttcaacattt tatgcccagg gcggtatagc
      181 cgccgtgttt gatgagactg acagcattga ctcgcatgtg gaagacacat tgattgccgg
      241 ggctggtatt tgcgatcgtc atgcagttga atttgtcgcc agcaatgcac gatcctgtgt
      301 gcaatggcta atcgaccagg gggtgttgtt tgatacccac attcaaccga atggtgcaga
      361 aagctaccat ctgacccgtg aaggtggaca tagtcaccgt cgaattcttc atgccgccga
      421 cgccaccggt agagaagtag aaaccacgct ggtgagcaag gcgctgaacc atccgaatat
      481 tcgcgtgctg gagcgcagca acgcggttga tctgattgtt tctgacaaaa ttggcctgcc
      541 gggcacgcga cgggttgttg gcgcgtgggt atggaaccga aataaagaaa cggtggaaac
      601 ctgccacgca aaagcagtgg tactggcaac cggcggtgcg tcgaaggttt atcagtacac
      661 caccaatccg gatatttctt ctggcgatgg cattgctatg gcgtggcgcg caggctgccg
      721 ggttgccaat ctcgaattta atcagttcca ccctaccgca ctgtatcacc cacaggcacg
      781 caatttcctg ttaacagaag cactgcgcgg cgaaggcgct tatctcaagc gcccggatgg
      841 tacgcgtttt atgcccgatt ttgatgatcg cggcgaactg gccccgcgcg atattgtcgc
      901 ccgcgctatt gaccatgaaa tgaaacgcct cggcgcggat tgtatgttcc tcgacatcag
      961 ccataagccc gccgatttta ttcgccagca tttcccgatg atttatgaaa aattgctcgg
     1021 gctggggatt gatctcacac aagaaccagt accgattgtg cctgctgcac actatacctg
     1081 cggtggtgtg atggtggatg atcatgggcg tacggacgtc gagggtttgt atgccattgg
     1141 cgaagtgagt tataccggct tacacggcgc taaccgcatg gcctctaact ccttgctgga
     1201 gtgtctggtc tatggctggt cggcggcaga agatatcacc agatgtatgc cttatgccca
     1261 cggcgtcagt acgttaccgc cgtgggatga aagccgcgtt gagaaccctg atgaacgggt
     1321 ggtgattcaa cataactggc acgagctacg tctgtttatg tgggattacg tcggcattgt
     1381 gcgcacaacg aagcgcctgg aacgcgccct gcggcggata accatgctcc agcaagaaat
     1441 agacgaatat tacgcccatt tccgcgtctc aaataatttg ctggagctgc gtaatctggt
     1501 acaggttgcc gagttgattg ttcgctgtgc aatgatgcgt aaagagagtc gggggttgca
     1561 tttcacgctg gattatccgg aactgctcac ccattccggt ccgtcgatcc tttcccccgg
     1621 caatcattac ataaacagat aaaaagcctg ggtcagcgcc gtatacgctt cggaatagtt
     1681 ctggtctggc ccacgaatga ctaagcgatc gctaaagcat tctcccgcct gcggggagaa
     1741 tgccagcagc acccgatgcg gcagtcgcgc ttcgttttcc gccacatccg tccgcaaacg
     1801 taaatgccag cccatactta atgccagttc agtaaaacca ttaccaatct gctctggcag
     1861 caccacgcag aaaaatccct cttcggtaat gcactccgcc gcacaggtca gcaacgatgg
     1921 gtgatcaagc gtagtggtat agcgagcctg ttcccgttga ggtgtcgcgc actctactcc
     1981 ctgctgatag taaggtgggt tactgatgat taaatcgaag cgtactgtct gctgtgtgat
     2041 ccactgctga atatccgccg tatggacgtt aatccgctct gcccacgggg actggttgat
     2101 attttcctgc gcctgcgcgg cagcttcact ttccagttca actgcatcaa tcattacgct
     2161 gtcatcggtt cgctgcgcca gcattaatgc cagcaacccg ctacccgcgc cgatatcaag
     2221 gcaacgtttt accccagcca ccggtgccca tgcgcccagc aaaatgccat ccgttcccac
     2281 tttcatcgca caacgatcgt gagcaacaaa aaactgttta aaagtaaatc cattacgacg
     2341 aagcacggat gtagactgtg acatgaaaat aaaaccttgc aggaaaaacg gcgatagcac
     2401 cgggtgagaa caatacccga gaagcgatat ccatacaaac agatgaagat tgcagccgta
     2461 acgtctataa tcagcgcccc acacagaggt agaacatgac tgtaacgact ttttccgaac
     2521 ttgaactcga cgaaagcctg ctggaagccc tccaggataa aggtttcact cgcccgaccg
     2581 ccattcaggc tgccgccatt ccgcctgcgc tcgatggccg tgatgtactc ggttctgcgc
     2641 cgacaggcac cggtaaaacg gcggcgtatc tgctgccagc gttgcagcac ctgctcgatt
     2701 tcccacgtaa gaaatccggt ccgccgcgta ttttgatcct caccccaact cgcgagctgg
     2761 cgatgcaggt ggccgatcat gcccgcgaac tggcgaaaca tacgcatctg gatattgcca
     2821 ccatcaccgg cggcgtagcc tatatgaacc acgcggaagt gttcagcgaa aatcaggaca
     2881 tcgtggtcgc cacgaccgga cgtctgctgc aatacataaa agaagagaac ttcgattgcc
     2941 gcgcggttga aacgctgatc ctcgacgaag cagaccgtat gctggatatg ggcttcgccc
     3001 aggatatcga acatattgct ggcgaaacgc gctggcgtaa acagaccctg ctcttttcgg
     3061 caactctgga aggcgatgcg attcaggact ttgccgagcg tctgctggaa gatccggtgg
     3121 aggtttctgc caatccgtcc acccgtgagc gcaaaaaaat tcatcagtgg tattaccgcg
     3181 ccgatgatct tgagcataaa accgcgttgc tggtgcatct gttaaaacag ccggaagcga
     3241 cccgctcaat tgtgtttgtg cgtaagcgtg agcgtgtgca tgagctggca aactggctgc
     3301 gcgaagcagg catcaacaac tgctatctcg aaggtgagat ggtacaaggc aagcgtaacg
     3361 aagcgattaa acgcttgacc gaaggccgcg taaacgtact ggttgctacc gatgttgccg
     3421 cgcgcggtat cgacattcct gacgtcagcc acgtctttaa cttcgatatg ccgcgcagtg
     3481 gcgatactta tttgcaccgt atcggacgta ccgcgcgcgc cggtcgtaaa ggcaccgcga
     3541 tttcgctggt ggaagcccat gaccatctgc tgctgggcaa agttggccgt tatattgaag
     3601 agccaattaa agctcgcgta attgatgagt tacgtccgaa aacgcgtgcg ccaagcgaga
     3661 agcagaccgg caagccatcg aagaaagtgt tggctaaacg tgccgagaag aaaaaagcta
     3721 aagagaaaga gaagccgcgg gtgaaaaaac gccatcgcga cactaaaaat attggtaagc
     3781 gccgtaaacc aagcggaacg ggcgtgccac cgcaaacgac agaagagtaa tctcaatgcc
     3841 gggtttaagc ccggcattgt ttttatcccg gcaaaaaaca ctcttcaaca cactgaataa
     3901 aagtgtgcat cgccgggcta accgcttttc cagcatggtg tgcgcacatg gccgtaatgg
     3961 tctgcgactg ttcgccaaaa ggcaattcga ttaattcgcc actcgcaagt tcatttgcca
     4021 ctgtaaagcg cggcaggtaa ctgaccccga tatttgccgc gacacaacgc ttgatgcttt
     4081 cgatacttat aagctcaatg gtgttttcca ccgtgatccg tcgctggcgc agcgtgctct
     4141 caaatatctg ccggaagaca cattgtggtt cgttgataat aaagctacag gcgttatgtc
     4201 ttcccggctc agtaaaatcg acatctgcaa tttgcggtga agccaccagc accagcggtt
     4261 gttcacccaa ctctcgtcgg ttcaaagcat catcattccc tacgcgataa aaaaccccga
     4321 catcggcctc atcattcagt agcgcatcac ggatcacgta acagttcagc gactgcaacg
     4381 ataaacgcac ttttggagcg cgttgccgaa aacgctgcaa cacctgcggc attcgataag
     4441 agagcaacgt ttcgcctgaa ataacgcgta gttccccgtc cggatccgac tcttttttgg
     4501 cagcctcacg aagggtatcc atcactctgg tgagttcata aatgtgcggc agcagctttt
     4561 ttccttcgcg ggtaaggcac attcgtcgac caattttctc aaataactgg actgagaact
     4621 cctgctcaag ctgctgaata tgaaaagtca ccgtcgattg tgtgcagcac aatttttgcg
     4681 aagctcgcaa aaaggaaccc tcttccacca cggttttaag tgtaataaaa cggcgcagat
     4741 ccataacccc aaacctatcg aaaatatcga atttagaatg taaaaacatt cattttttta
     4801 aatgttccgt gtcgggtact gtctaccaaa acagaggaga taacaagtga caccgaccct
     4861 tttaagtgct ttttggactt acaccctgat taccgccatg acgccaggac cgaacaatat
     4921 tctcgccctt agctcagcca caacgcacgg atttcgtcag agtactcgcg tgctggcagg
     4981 gatgagtctg ggatttttaa tcgtcatgtt gctatgcgcg ggaatttcgt tttcactggc
     5041 tgttatcgac ccggcagcag tgcatctgtt gagttgggcg ggggcggcat atattgtctg
     5101 gctggcgtgg aaaatcgcta ccagtccaac aaaggaagat gggcttcaga caaaaccaat
     5161 cagtttttgg gccagctttg ctttgcagtt tgtgaacgtc aaaatcattt tgtatggcat
     5221 tacagcactg tcgacgtttg tcctgccgca aacgcaggcg ctaagctgga taattggcgt
     5281 cagcgttttg ctggcgatga ttgggacgtt tggcaatgtg tgctgggcgc tggcggggca
     5341 cctgtttcag cgattgtttc gccagtatgg tcgccagtta aatatcgtgc tcgcgctgct
     5401 gctggtctat tgcgcggtac gcattttcta ttaacgaaaa aaagcggaag aagtcgccct
     5461 cttccgctta gtaacttgct acttaagcct tacaggcttt cagtaaaggt acgagcaata
     5521 acgtcgcgct gctgttccgg agtcagagag ttaaagcgaa ctgcataacc ggatacacgg
     5581 atggtcagct gcggatattt ttccggatgc ttaactgcat cttccagagt ttcacgacgc
     5641 agaacgttta cgttcagatg ttgaccacct tcaacgcgaa cttctggttt cacttctact
     5701 ggaacttcac ggtactcaat gtcacccagt ttgcttactg caaccacttc gtcttctgca
     5761 aaacctgctt tcgcaacgat gcaacgcgct tcgccttttt cgctgtccag cagccagaaa
     5821 gagttcagca gatcgtcgtt agcggcttta gtaatctgga tacctgtaat catgtgatgc
     5881 ctccccggca aaattatttg atttgttcag cctgtcgcgg ccaattggta aaaccattgt
     5941 tgcttgagtg tatatatact cctcaaacac ccttgaatct ttgatttaaa tcaataaaaa
     6001 ccacacatca agtatggttg caaatggatt ttattgtttt acatcaactt atgcaggtgt
     6061 gaaattttac caatttacat ttttttgcac tcgtttaagt ctaaaaaatg agcatgattt
     6121 tgttctgtag aaagaagcag ttaagctagg cggattgaag attcgcggga gagcgagatg
     6181 gcaaacgaat taacctggca tgatgtgctg gctgaagaga agcagcaacc ctattttctt
     6241 aataccctac agaccgtcgc cagcgagcgg cagtccggcg tcactatcta tccgccacaa
     6301 aaagatgtct ttaacgcgtt ccgctttaca gagttgggtg acgttaaagt ggtgattctc
     6361 ggccaggatc cttatcacgg accgggacag gcgcatggtc tggcattttc cgttcgcccc
     6421 ggcattgcga caccgccgtc actgctgaat atgtataaag agctggaaaa tactattccc
     6481 ggtttcaccc gccctaatca tggttatctt gaaagctggg cgcgtcaggg cgttctgcta
     6541 ctcaatactg tgttgacggt tcgcgcaggt caggcacatt cccacgccag cctcggctgg
     6601 gagaccttca ccgataaagt aatcagcctg attaaccagc accgcgaaga cgtggtgttt
     6661 ttgttgtggg gctcacatgc gcaaaagaaa ggggcgatta ttgataagca acgccatcac
     6721 atactgaaag cgccgcatcc atcaccgctt tctgcgcatc gtgggttctt tggctgcaac
     6781 cattttgtgc tggcaaatca gtggctggaa caacgtggcg agaaaccgat tgactggatg
     6841 ccagtattac cggcagaaag tgagtaaatt tgcaggaaaa cgccggatgg cagcgttgcc
     6901 acccggctga tttatcaggc tttattctga cgccaccatt caccaagcaa aacgccggtt
     6961 gcaacagaga tatttagccc ggcaacgttg ccagtaccat caatcttcac gcgcaaatcg
     7021 ttcggatcgc gtgcggcatc cggtaaccct tcatattcct gacccagcac cagtaccatt
     7081 ttcgctggca gactggtttt gaacagcggt ttcccctgct cgctggaagt ggtcactacg
     7141 gtgtaacctg cctgacggaa atcatccagc acgttaacaa tgttgtcgcc agtaatcggc
     7201 tgaacgtgct ctgcgccgcc ttctgcggta cggatagccg cccccgactc cagcagtgcc
     7261 gcatcctgca ccacaacgcc tttcacgccg aagtgcgcgc agctgcgcat catgccaccc
     7321 aggttatgcg ggttagattc gttttccagc gccagtacac aatcctgcgc gcctgcctgg
     7381 cttacccact gcagcacggt tgtaccgtta cgctttttga tcaggaagca aacgccgccg
     7441 tgatgttccg tgcctgacgc ttttgtcagt tccgcttcat ctaccacatg gtacgctttg
     7501 cggtttgctg ccatccagcg caaggcttct ttaaaacgcg gcgttacact ctggataaac
     7561 caggcgcgaa caatcgcttc cggacggctc tggaacagtg cctgacaggc attttcgccg
     7621 tagacgcggg tttcttccgc acgctgacga cgcaacactt ccggatcaat aaaactttta
     7681 ccactgatgc caccgtgatc ggccttttcc ggcgtctcat cacccggcgc gcgggaaact
     7741 gtgcgccacg gcgactcttc ccacttgcgg tcacggggct gactctgttt gtcatcgcgg
     7801 gcggggcgac ggccaccgtc agcacgagat tttcctggac gcccgccccc tttcccggta
     7861 cgcgggttgt gggtacgttt atcagaatca tcatcactgc ggacatacat cactttgacc
     7921 ttgccgcttt tacctttcat ttcatcgttc atgcttttct ccaccagcgc tgcgcgaagc
     7981 gcgcagatta cccgaagtcc gcgcggttcg ccatgatttc gtaccaaagc ctgcgactat
     8041 catacctatt gaataaaaca gattgttgtc tggaacaatg tccccgataa tatgtaacat
     8101 attagaaaca taccggcgtc gttgccgata agtctcctta ctcatcccga ggttagttat
     8161 gaataccgtt tgtacccatt gtcaggccat caatcgcatt cccgacgatc ggatcgaaga
     8221 tgcggcaaaa tgcggacgct gcggtcacga cttgtttgac ggagaggtga ttaatgcgac
     8281 cggtgaaacg ctcgacaaat tgctgaaaga tgatctacct gtggtgatcg acttctgggc
     8341 accgtggtgc ggcccctgcc gtaatttcgc accaattttt gaagatgtcg cgcaagagcg
     8401 tagcggtaaa gtgcgctttg tgaaagtgaa taccgaagct gaacgtgaat tgagcagtcg
     8461 ctttggaatt cgtagtatac cgacgatcat gattttcaaa aacggtcagg ttgtcgacat
     8521 gcttaatggc gcagtaccga aagcgccgtt tgatagctgg ctgaacgaat ctctttaatc
     8581 ttaccggggc gcatcttgtg ccccgttttc tcctctgcga caatggcgtt ttttcgacgc
     8641 tctcttatga ccgaaaacgc tgttctccag ttacgcgccg agcgtattgc gcgcgcaaca
     8701 cgtccttttc ttgcccgcgg taatcgcgtt cgtcgctgcc aacgctgtct tttgccagag
     8761 acattatgtc tctgttcgac aattacccca gcacaagcaa aaagtcgctt ctgtttgctg
     8821 atgttcgaca ccgagccaat gaagcccagt aataccgggc gtctgattgc tgatattttg
     8881 cctgataccg ttgcgtttca atggtcgcgt accgaaccct cgctagattt gctggatctg
     8941 gtacaaaacc cggactatca gccaatggtg gtctttcccg cctcgtatgc tgatgagcaa
     9001 cgggaagtga tcttcacacc acctgccggt aagcctccgc tatttatcat gctcgatggt
     9061 acctggcctg aagctcgcaa gatgtttcgt aaaagtccgt atctggataa tcttcccgtc
     9121 atttccgtcg atctttcccg gctttctgcc tatcgcctgc gtgaagccca ggctgaaggc
     9181 caatattgta ctgccgaggt agccatcgca ctgttagata tggccggcga taccgaagcc
     9241 gcaacgggct taggcgagca tttcactcgt tttaaaacgc gctatctggc aggaaaaacg
     9301 caacatctgg gtagcatcac agcagaacag ttagaaagcg tttaaaatca ttcggtcact
     9361 tctgcgggag accggtatga gtcagcgagg actggaagca ctactgcgac caaaatcgat
     9421 tgcggtaatt ggcgcatcga tgaaatccaa tcgagcaggt tacctgatga tgcgtaacct
     9481 gctggcggga ggctttaacg gaccggtact cccggtgacg ccagcctgga aagcggtgtt
     9541 gggtgtgttg gcctggccgg atattgccag cttgcccttt acacccgacc ttgcggtttt
     9601 atgtaccaat gccagccgta atcttgctct tctggaagag ctcggcgaga aaggctgtaa
     9661 aacctgcatt attctttccg ccccggcatc gcaacatgaa gatctccgcg cctgcgccct
     9721 gcgccataac atgcgcctgc taggaccaaa cagtctgggg ttactggctc cctggcaagg
     9781 tctgaatgcc agcttttcgc ctgtgccgat taaacgcggc aagctggcgt ttatttcgca
     9841 atcggctgcc gtctccaata ccatcctcga ctgggcgcaa cagcgtgaga tgggcttttc
     9901 ctactttatt gcgctcggcg acagcctgga tatcgacgtt gatgaattgc tcgactatct
     9961 ggcccgcgac agtaaaacca gcgccatcct gctctatctc gaacagttaa gcgacgcacg
    10021 acgctttgtt tcggcggccc gtagtgcctc tcgcaacaaa ccgattctgg tgattaaaag
    10081 cggacgtagc ccggcggctc aacgtctgtt gaacaccacg gcaggaatgg atccggcgtg
    10141 ggatgcggct attcagcgtg ccggtttgtt gcgggtacag gatactcacg agctgttttc
    10201 ggcggtggaa accctcagcc atatgcgccc gctgcgtggc gaccggctga tgattatcag
    10261 caacggtgct gcgcctgccg cgctggcgct ggatgcctta tggtcacgca atggcaagct
    10321 ggcaacgcta agcgaagaaa cctgccagaa actgcgcgat gcactgccag aacatgtggc
    10381 aatctctaac ccgctcgatc tacgcgatga cgccagcagt gagcactata ttaaaacgct
    10441 ggatattctg ctccacagcc aggattttga cgcgctgatg gttattcatt cgcccagcgc
    10501 cgctgctccc gcaacagaaa gtgcgcaagc attaattgaa gcggtaaagc atcatccccg
    10561 cagcaagtat gtttctctgc tgacgaactg gtgcggcgag cactcctcgc aagaggcacg
    10621 acgattattc agtgaagccg ggctgccgac ctatcgcacg ccggaaggaa ccatcactgc
    10681 ttttatgcat atggtggagt accgacgtaa tcagaagcaa ctacgcgaaa cgccggcgtt
    10741 gcccagcaat ctgacctcta ataccgcaga agcgcatctt ctgttgcaac aggcgattgc
    10801 cgaaggggct acgtcgctcg atacccatga agttcagccc atcctgcaag cgtatggcat
    10861 gaacacgctc cccacctgga ttgccagcga tagcaccgaa gcggtgcata ttgccgaaca
    10921 gattggttat ccggtggcgc tgaaattgcg ttcgccggat attccacata aatcggaagt
    10981 tcagggcgta atgctctacc tgcgtacagc taatgaagtc cagcaagcgg caaacgctat
    11041 tttcgatcgc gtaaaaatgg cctggccgca ggcgcggatc cacggcctgt tggtgcaaag
    11101 tatggctaac cgtgctggcg ctcaggagtt gcgggttgtg gttgagcacg atccggtttt
    11161 cgggccgttg atcatgctgg gcgaaggcgg tgtggagtgg cgtcctgaag atcaagccgt
    11221 cgtcgcacta ccgccgctga acatgaacct ggcccgctat ctggttattc aggggatcaa
    11281 aagtaaaaag attcgtgcgc gcagtgcgct acgcccatta gatgttgcag gcttgagcca
    11341 gcttctggtg caggtttcta acttgattgt cgattgcccg gaaattcagc gtctggatat
    11401 tcatcctttg ctggcttctg gcagtgaatt taccgcgctg gatgtcacgc tggatatcgc
    11461 gccgtttgaa ggcgataacg agagtcggct ggcagtgcgc ccttatccgc atcagctgga
    11521 agaatgggta gaattgaaaa acggtgaacg ctgcttgttc cgcccgattt tgccagaaga
    11581 tgagccacag cttcagcaat tcatttcgcg agtcaccaaa gaagatcttt attaccgcta
    11641 ctttagcgag atcaacgaat ttacccatga agatttagcc aacatgacgc agatcgacta
    11701 cgatcgggaa atggcttttg tggcggtacg acgtattgat caaacagaag agatcctcgg
    11761 cgtcacgcgt gcgatctccg accctgataa catcgatgcc gaatttgccg tgctggttcg
    11821 ctcggatctc aaagggttag gcttaggtcg acgcttaatg gaaaagttga ttacctatac
    11881 gcgagatcac ggactacaac gtctgaatgg tattacgatg ccaaacaatc gtggcatggt
    11941 ggcgctggcc cgcaagctcg ggtttaacgt tgatatccag ctcgaagagg ggatcgttgg
    12001 gcttacgcta aatcttgccc agcgcgagga atcatgagta aggtactgga aatgttgacc
    12061 acttaatcgg gactggtgtt attattgccc gcttatgtcg tctgcattgc acagaggacc
    12121 cttcaatgaa cagagaagaa atgcactgtg atgttgtcaa aatttaagcg taataaacat
    12181 caacaacacc ttgcccaact acccaagatt tctcaatcag ttgatgatgt cgatttcttt
    12241 tacgctcccg ccgacttccg ggagacgttg ctggaaaaaa tagccagcgc gaagcagcgc
    12301 atttgcattg ttgccctgta tctcgaacag gatgacggtg gcaaaggcat tctgaacgcg
    12361 ttgtatgagg ctaaaaggca gcgtccggaa ctggatgtgc gggtgctggt cgactggcat
    12421 cgtgcacaac gtggacgcat tggcgctgcg gcatctaaca ctaacgctga ctggtactgc
    12481 cgcatggcgc aggaaaatcc gggcgtagat gttccggttt atggcgttcc aatcaatacc
    12541 cgtgaagccc ttggtgttct gcactttaaa ggctttatca tcgacgatag cgtactttat
    12601 agcggtgcca gcctgaacga tgtttacctg catcagcacg ataaatatcg ctacgaccgt
    12661 tatcatctaa tccgtaaccg taagatgtca gacattatgt ttgaatgggt tacacagaat
    12721 attatgaatg gccgcggcgt taatcgtctg gatgatgtta atcggccaaa aagcccggaa
    12781 atcaagaacg acattcgcct gttccgccag gagctgcgtg atgccgctta tcatttccag
    12841 ggcgatgccg acaacgatca gctttccgta acgccgctag tggggctggg gaaatcgagt
    12901 ctgttgaaca agaccatttt ccatcttatg ccttgtgccg agcagaaact aaccatctgt
    12961 acgccatact tcaacctgcc agcaatcctt gtgcgcaata ttatccagtt gctgcgcgaa
    13021 gggaaaaagg tcgaaattat tgttggtgat aaaaccgcga acgacttcta cattccggaa
    13081 gatgaacctt tcaagataat tggcgcattg ccttatctct atgagatcaa tctgcgtcgt
    13141 ttcctgagcc gtttgcagta ttacgtcaat actgaccagc tagtggttcg gttatggaaa
    13201 gatgacgaca acacctatca cctaaaaggg atgtgggttg atgataagtg gatgttgatc
    13261 accggtaata acctgaaccc gcgcgcctgg cgtctggatc tggaaaacgc cattttgatc
    13321 cacgatccgc aactggagct tgcgccacag cgagagaaag aactggagct gatccgcgag
    13381 cataccacca tcgttaagca ctatcgcgat ctgcaaagta ttgccgatta tccggtgaag
    13441 gttcgtaaac tcatccgtcg tttgcgccgt atccgcatcg accgattaat tagccgcatc
    13501 ctgtaatcac aaccccgtcc tgtacggggt ttgttttgga ggccacgttt tgcgtattct
    13561 ttttgtctgt tcgctattgt tactttctgg atgcagccat atggctaacg atagctggag
    13621 cgggcaggat aaagctcaac actttatcgc ctcggcgatg ctttccgccg ccggaaatga
    13681 atatgcacag catcagggga tgagccggga tcgcagtgcc atgtttggat tgatgttctc
    13741 tgtcagtctg ggggcgtcaa aagagctttg ggatagccgc cctgagggga gcggctggag
    13801 ctggaaggat tttgcctggg atatcgccgg tgcaagcacc ggctataccg tctggcaact
    13861 gacccgtcac taaagccgca tcccttttcc tttacggtgc aatgttagtg aaaccagaaa
    13921 tgccaccacg gccatcaagg tcacatacca gaagaaggct gtttccattc ctattgattt
    13981 cagcgacaac gctacgtact ccgccgaccc accaaatata gcattggcga ccgcatatga
    14041 cagaccaacg cctaatgcgc gaacctgtgc cgggaacatc tcagccttca gtattccact
    14101 gattgaagta taaaaactca ctatcagcag ggcgcacatc accagaccaa aagcggcata
    14161 aggcgaggaa acgttctgca atgctgagag aataggaacg gtaaaaatgg ctgccagaga
    14221 accgaaacat aacattgagg tacgacgacc aatcttatcc gacagcgcgc caataagtgg
    14281 ttgaataagc atgaatacaa acaatgcggc agtcataatg ccactcgcca cgttggcatg
    14341 cattcccgca gtatttacca gatacttctg catataagtg gtgaaggtat agaaacaaag
    14401 ggagcccgca gcggtaaaac cgagaaccat gatgaatgca cggcgattgc gccataatcc
    14461 tttcagagat ccagcttctt ttaaagcgcg tgtttcttgt tgtgaagttt catctaactg
    14521 acgacgtaac cacaacgcca caacagctaa cacagctcct aacgcgaaag gaatacgcca
    14581 tccccactct ctaagtgcag cgtcttccat ggtgtgttgt aaaaccacga caaccagtag
    14641 agctagcagt tgcccgccga tcaacgtcac atactgaaat gatgcgtaaa aacctttgcg
    14701 cccttcaacg gcaacttcac tcatataggt ggcgctggtg ccatattctc cgccaacgga
    14761 taatccctga aataaacgag cgagaagcaa taatgccgga gcccacgtac ctatagtttc
    14821 ataacctggg aggcaggcga taaccagcga tccgaaacac atcatacaca ccgataacag
    14881 catcgatttt ttgcgaccat gtttatcggc tatgcggcca aatagccaac cgcctattgg
    14941 gcgcatcagg aatcccgcag caaaaacacc tgctgtttgc agtagttgag tcgtcgtgtt
    15001 cccggaaggg aagaagatgt gggcgaagta gagtgaacag aacgagtaga catagaaatc
    15061 gaaccactcg accagattac ctgaagaggc ccccacaatt gcccaaatgc ggcgacgagt
    15121 atcactactt gtcagtttgc tgtctgccgt tacagtactt tcagccatgc cattatgtct
    15181 cctgccgtaa tccgatgctt ttgtcggtcg cttttgttta tttctattgg taaagaaaat
    15241 gttatacatt tgttgcatat cattatgcaa ccttaaccac gaatttagtt agcaggaaaa
    15301 tggttattga ggagcttaag gataaatttc tggtaaggag gacacgtatg gaggtgggca
    15361 cgttggggaa gccgtattcg ctgctgaatc tagcttatgt gggagtataa gacgcgccag
    15421 cgtcgcatcc gg
//
LOCUS       DAZUCC010000119        15304 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZUCC010000119 DAZUCC010000000
VERSION     DAZUCC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.119"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            299..744
                     /locus_tag="ACHY09_003120"
                     /pseudo
     CDS             299..744
                     /locus_tag="ACHY09_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
     gene            788..1056
                     /locus_tag="ACHY09_003121"
                     /pseudo
     CDS             788..1056
                     /locus_tag="ACHY09_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1261..2379
                     /gene="ybdK"
                     /locus_tag="ACHY09_003122"
     CDS             1261..2379
                     /gene="ybdK"
                     /locus_tag="ACHY09_003122"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFS3886063.1"
                     /translation="MPIPDFQVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLMEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            2445..2693
                     /gene="ybdJ"
                     /locus_tag="ACHY09_003123"
     CDS             2445..2693
                     /gene="ybdJ"
                     /locus_tag="ACHY09_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFS3886064.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2758..3126
                     /gene="ybdF"
                     /locus_tag="ACHY09_003124"
     CDS             2758..3126
                     /gene="ybdF"
                     /locus_tag="ACHY09_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS3886065.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            3220..3873
                     /gene="nfsB"
                     /locus_tag="ACHY09_003125"
     CDS             3220..3873
                     /gene="nfsB"
                     /locus_tag="ACHY09_003125"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFS3886066.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQVKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLEEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3981..5228
                     /gene="ybdG"
                     /locus_tag="ACHY09_003126"
     CDS             3981..5228
                     /gene="ybdG"
                     /locus_tag="ACHY09_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFS3886067.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(5309..6685)
                     /gene="pheP"
                     /locus_tag="ACHY09_003127"
     CDS             complement(5309..6685)
                     /gene="pheP"
                     /locus_tag="ACHY09_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFS3886068.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6787..9930)
                     /gene="cusA"
                     /locus_tag="ACHY09_003128"
     CDS             complement(6787..9930)
                     /gene="cusA"
                     /locus_tag="ACHY09_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFS3886069.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGQTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9942..11165)
                     /gene="cusB"
                     /locus_tag="ACHY09_003129"
     CDS             complement(9942..11165)
                     /gene="cusB"
                     /locus_tag="ACHY09_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFS3886070.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADESLKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(11181..11513)
                     /gene="cusF"
                     /locus_tag="ACHY09_003130"
     CDS             complement(11181..11513)
                     /gene="cusF"
                     /locus_tag="ACHY09_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFS3886071.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKIIIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(11537..12919)
                     /gene="cusC"
                     /locus_tag="ACHY09_003131"
     CDS             complement(11537..12919)
                     /gene="cusC"
                     /locus_tag="ACHY09_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFS3886072.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSNNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            13076..13759
                     /gene="cusR"
                     /locus_tag="ACHY09_003132"
     CDS             13076..13759
                     /gene="cusR"
                     /locus_tag="ACHY09_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFS3886073.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13749..15197
                     /gene="cusS"
                     /locus_tag="ACHY09_003133"
     CDS             13749..15197
                     /gene="cusS"
                     /locus_tag="ACHY09_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFS3886074.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQAQRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFMIKLPEKG"
BASE COUNT         3717 a         4098 c         3749 g         3740 t
ORIGIN      
        1 aagtgtttct tgagtgacat aaaataagtg tggtgtccat atcctcctat cgtgaatcaa
       61 tataaatgat ttacacctaa cactataaaa tgttcagaaa gccatcgcaa taataaaaaa
      121 taagacatta cgataatatt ataaagcatt gctttgttgg gggatgacgt atgtagcaat
      181 gaaagataac aaatgccggt aatggtacgg agaaatcctc agtaataggc gggtaaaaaa
      241 gacgggcata gagttttgtg gtcgactggt tgtgctaaca attcaggaga tcacctctat
      301 gcccgttcgc tcatcatgcc aaaagttttt ccgtgatgat ttgactccat ttcatcagta
      361 ccgccagaat gccatccttg acgccactgc tgcgcttagt cgggacgcat ccctcatcct
      421 taccagcatt ggacggtata tgccaggcaa tgctcaggtt aaaacaagat taagcccatc
      481 gatcgcctgc caggcaatga cgctttacac cgcgatatcc cggctatttt tcataatatt
      541 acccggtttc agccccgaga ccttacgttg tgcgtcattg ccgttgactg gagtggttat
      601 ccttctcagg cattccatgt tctgcatact ccccaagttt gcgacgggcg ttctatatta
      661 cttctggatc aggttgtatc gtcattctgg caacaaattg ctcagttcta taagaatttt
      721 cttgatacgc ttgcaatcac tattgctccg aataaaccgg ataaaaatta cctttaaaca
      781 cagcaccatg accgccacaa gggcatattt cgtcagcatg tttatattgc ctccatgcaa
      841 agaagggact accatccaaa ttgtttgggt ttggagtggc agccccagga ttgatagaaa
      901 tatctcctgg ggcttcatct ttccagacct cagagtaaac ctgaaaccgg aaagcttcag
      961 gcacccgccg ttatcttacc cttccttagc aaaatggctg tttatttata cagtaagtcc
     1021 tgtttcggac ttctcaatcc tcatttgatc aactgaagct gaacccgttt tattctgcat
     1081 gcctcaatcg tcaaaattct tcgccaccag tccgcactgc cataacttgt catattgcaa
     1141 aatgtagcca atttgcttca gtcaccatca tcaaaaaaag aaacattcca gaacttccgc
     1201 atctcgccca gccgctccga cttctatact gaatagaaaa cgccaacata agagaaacct
     1261 atgccaatac ccgattttca agtctctgaa ccttttaccc tcggtattga actggaaatg
     1321 caggtggtta atccgccggg ctatgactta agccaggact cttcaatgct gattgacgcg
     1381 gttaaaaata agatcacggc cggagaggta aagcacgata tcaccgaaag tatgctggag
     1441 ctggcgacgg atgtttgccg tgatatcaac caggctgccg ggcaattttc agcgatgcag
     1501 aaagtcgtat tgcaggcagc cgcagaccat catctggaaa tttgcggcgg tggcacgcac
     1561 ccgtttcaga aatggcagcg tcaggaggta tgcgataacg aacgctatca acgaacgctg
     1621 gaaaactttg gctatctcat ccagcaggcg accgtttttg gtcagcatgt ccatgttggc
     1681 tgcgccagtg gcgatgatgc catttatttg ctgcacggcc tgtcacggtt tgtgccgcac
     1741 tttatcgccc tttccgccgc gtcgccatat atgcaaggaa cggatacgcg ttttgcctcc
     1801 tcacgaccga atattttttc cgcctttccc gataatggcc cgatgccgtg ggtcagtaac
     1861 tggcaacaat ttgaagccct gtttcgctgc ttgagttaca ccacgatgat cgacagcatt
     1921 aaagatctgc actgggatat tcgccccagt cctcattttg gcacggtgga ggttcgggta
     1981 atggataccc cgttaaccct tagccatgcg gtaaatatgg cgggattaat tcaggctact
     2041 gcccactggt tactgacaga acgcccgttc aaacatcagg agaaagatta cctgctgtat
     2101 aaattcaacc gtttccaggc ctgtcgctat gggctggaag gcgtcattac cgatccgcat
     2161 actggcgatc gtcggtcact aatggaagac accttgcgtt tactggaaaa aatcgcccct
     2221 tctgcacata aaattggcgc atcgagcgca atagaagcac tgcaccgcca ggtcgtcagc
     2281 ggtctgaatg aagcgcagct gatgcgcgat ttcgtcgccg atggcggctc gctgattggg
     2341 ctggtgaaaa agcattgtga gatctgggct ggtgactaag ccgggaattg ccttacagaa
     2401 gaatatccct gacaatggtg ttttaactta catttaacaa tgttatgaaa caccctttag
     2461 aaaccttgac caccgcagca ggcattttgc tgatggcttt cctttcttgc ctgctgctgc
     2521 ccgcccccgc actggggctg acgctggcac aaaaactggt gaccatgttc catctgatgg
     2581 atcttagcca gctttacact ttattgtttt gtctgtggtt tttagtgctg ggcgctattg
     2641 agtattttgt tctgcgcttt atctggcgac gctggttctc gctggcggat taaacgtcga
     2701 ttatcgccgt acttcgcggc atactttgct tattctcctt cgcctaaagg aacgtttatg
     2761 gataagcaat cactgcacga aacggcgaaa cgcctggccc ttgagttacc ctttgtcgag
     2821 ctttgctggc cttttggccc ggagttcgat gtttttaaaa ttggcggcaa gatttttatg
     2881 ctgtcgtcgg agctgcgcgg cgtccccttt atcaatctga agtccgatcc acaaaaatcc
     2941 ctgttaaatc agcaaatata cccgagcatc aagccagggt atcacatgaa taaaaagcac
     3001 tggatttcag tgtatcccgg cgaggaaatc tccgaagcgt tacttcgcga tctgatcaac
     3061 gattcgtgga atctggtggt tgatggtctg gctaaacgcg atcaaaaaag agtgcgtcca
     3121 ggctaaagcg gaaatctata gcgcattttt ctcgcttacc atttctcgtt gaaccttgta
     3181 atctgctggc acgcaaaatt actttcacat ggagtcttta tggatatcat ttctgtcgcc
     3241 ttaaagcgtc attccactaa ggcatttgat gccagcaaaa aactaacccc ggaacaggcc
     3301 gagcaggtca aaactctcct gcaatacagc ccatccagta ccaactccca gccgtggcat
     3361 tttattgttg ccagcacgga agaaggtaaa gcgcgtgttg ccaaatccgc tgccggtaat
     3421 tacgtgttca acgagcgtaa aatgcttgat gcctcgcacg tcgtggtgtt ctgtgcaaaa
     3481 accgcgatgg acgatgcctg gctgaagctg gttgttgacc aggaagatgc cgatggccgc
     3541 tttgccacgc cggaagcgaa agccgcgaac gataaaggtc gcaagttctt cgccgatatg
     3601 caccgtaaag atctgcacga tgatgcagag tggatggcaa aacaggttta tctcaacgtc
     3661 ggtaacttcc tgctcggcgt ggcggcgctg ggtctggacg cggtgcccat cgaaggtttt
     3721 gacgccgcca tcctcgatga agaatttggt ctggaagaga aaggctacac cagtctggtg
     3781 gttgttccgg tgggtcatca cagcgttgaa gattttaacg ctacgctgcc gaaatctcgt
     3841 ctgccgcaaa acattacctt aaccgaagtg taattctccc ttgccgggca tctgcccggc
     3901 tatttcccct cagattctcc tgatttgcat aaccctgttt cagccgtcat cataggctgc
     3961 tgttgtataa aggagacgtt atgcaggatt taatatccca ggttgaagat ttagcgggta
     4021 ttgagatcga tcacaccacc tcgatggtga tgattttcgg tattattttt ctgaccgccg
     4081 tcgtggtgca tatttttttg cactgggtgg tactgcggac cttcgaaaaa cgcgccatcg
     4141 ccagttcacg gctttggttg caaatcatta cccagaataa actcttccac cgtttagctt
     4201 ttaccctgca ggggattatc gtcaatattc aggcggtatt ctggctgcaa aaaggcaccg
     4261 aagcggcaga tattctgact acctgcgcgc agttgtggat catgatgtat gcgctgcttt
     4321 cagtcttctc gttgctggat gtgattttga atctggcgca gaaattcccg gcggcatctc
     4381 agttaccgct gaaagggata tttcagggga ttaaactgat cggcgcgatt ctggtcggca
     4441 ttttgatgat ctcgctgctg attggtcagt cgccagcgat tctgatcagc ggtcttggtg
     4501 caatggctgc cgtgctgatg ttggtattta aagatccgat tcttggtctg gtggcaggta
     4561 ttcagctttc cgcgaacgat atgctgaaac tgggcgactg gctggagatg ccgaaatacg
     4621 gcgcggatgg cgcggtgatc gatattgggt taaccaccgt caaagtacgt aactgggaca
     4681 ataccattac cactattccc acctggtctc tggtttctga ctcctttaaa aactggagcg
     4741 ggatgtcagc atctggcggg cgacgtatta agcgcagtat cagtattgat gtcaccagta
     4801 ttcgttttct tgatgaagac gaaatgcaac gtctgaataa agcgcatttg ttaaagcctt
     4861 atttaaccag ccgccatcag gaaattaatg agtggaatcg ccagcaaggt tctacggagt
     4921 cggtattaaa cctgcgccga atgaccaata ttggaacctt tcgtgcctat ctgaacgaat
     4981 atctacgtaa ccatccgcgg attcgtaaag atatgacctt aatggtacgc caactggctc
     5041 ctggcgataa cggtttaccg ctcgagatct atgcgtttac caacaccgtg gtgtggctgg
     5101 aatatgaaag cattcaggct gatatattcg accacatatt tgcgattgtc gaagagtttg
     5161 gtctgcgact tcatcagtcg ccaactggca atgatattcg ctctctggcg ggtgcattta
     5221 agcagtaatt aaaaaaaccg ctctcatcga atggatgaga gcggtttcgg atggttgaca
     5281 tcattttgtc ggatacggcg cgaatgcctt atttccgacg cagcgtttta aacgctacaa
     5341 acaggaatac aatccacact ggcagcagaa tcgctgacaa gcgcatatca tccatcgtgc
     5401 acatcagcag caaaatcatg ccgaggaagg cgatgcaaag atagttgccg aacggataga
     5461 gcagcgcctt aaactgtgtt tcacgtccct gacgtcgcat cgccgcgcga aaacgcagat
     5521 gcgccaggca gatcataatc cagttcaaca gcagcgttgc taccaccagc gccatcagca
     5581 gaccaaacgc tttttgcggc agcagatagt taatcagcac caccagcgaa gtgatggctc
     5641 cggaaagcat cagcgagtta atcggcacgc cgcgacggct gacgcgagtc aaaaacttcg
     5701 gtgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa accccgctgt
     5761 tatacaccga cagcgatgcc accagaatga cgaagttcag cgcagaagct accacgttgc
     5821 tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact tccacccacg
     5881 gatagagtgc cagtaaaacc accagtgaac cgatataaaa cagcagaatg cgatacacca
     5941 cctgattcac tgctttcgga atgctttttt ccggatcgcg cgcttcagcg gcagtaatcc
     6001 caatcagctc cagaccgccg aaagagaaca taattaccgc cagcgacaaa atcagcccat
     6061 tccagccggt ggcaaaaaaa ccaccgtaac gccagaggtt atcgatactg gctttctcgc
     6121 cgccgtgacc agaaaacagc agccacaggc caaagccgat cataccgatg atcgccagca
     6181 ctttaatcag cgcaaaccag aattcggttt cgccatataa gcgcacgttc accaggttaa
     6241 cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca tccgggaacc
     6301 agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc acgaacatta
     6361 cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta taggcaaagt
     6421 gggcaaatga accggatacc ggctcctcaa ccaccatttc gccaagctgg cgcataatca
     6481 ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc gccatctgaa
     6541 tcgccggacc aatgccaaga aacagaccag taccaattgc gccacccagc gcaatcagtt
     6601 gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac gcagtatctt
     6661 ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg ttgaggggca
     6721 ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa acggggtatc
     6781 ctgcttttat ttccgtaccc gatgtcggtg cagccacatc agcttatacg ccgccgggat
     6841 aataaacagc gacagcaaag gtgcggtgat catgccgcca atcatcggtg cggcaatccg
     6901 gctcatcacc tctgaaccag cccccgttcc ccacagaatc ggcagcagac cggcgataat
     6961 cactgccacc gtcatcgctt tcgggcgcac gcgcaggacc gcgccgtgat acaacgcctc
     7021 atccagcttc tgctcgctga atgtttgcgg attattcaac gacggctcgg cctctatggc
     7081 gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg gcaaccccgg caagggcaat
     7141 aaaaccggtg cccgtcgcca cggaaagatg aaagcccatc caccacagca accagatgcc
     7201 gcccaccagc gcaaacggta cgctgctgat aatcagcaac gcttcgccca cccgacggaa
     7261 cgccagatat aacagcacga agatgatcat cagcgtcatc ggcaccatga gtttcagctt
     7321 atggttggcg cgctccagta gctcgaactg cccggagaat gccacgctgg tgcccggttt
     7381 taactgcact ttttcagcta tcgctttttg cagatcgtga accaccgaca ccatgtcacg
     7441 atcgcgggca tcgatataaa tccagctcgt cgggcgcgca ttctcggttt tcagcatcga
     7501 cggtccggta gagactttaa cgtcagccac gtctgccagg gtgatttgct gcttcatcgg
     7561 cgtgaggatc ggcaactggc gcagtgcctg cgggctatcg cgccagcttt gcggataacg
     7621 cagattaatt ggataacgag caatcccttc caccgtttcg ccaaccatcg ccccgcccac
     7681 cgcagaagtc acaaacaact gcacatccgc caccgtcata ccgtaacgcg cggctttttc
     7741 acggttaatc tcaacgttga tataacgccc accttccaga cgctcagcaa gcgcagaagc
     7801 cacgcctggc accgttcgcg ccacttcttc aatttgctcc gccatcgtgt cgatatccgc
     7861 cagcacggtg ccggaaactt taataccgat gggactttta atgccagtcg agagcatatc
     7921 gatacggtta cgaattggcg gcacccacag attcgccagc cccggcaaac gcacggtgtt
     7981 atccagttcc tcaatgattt tgtccatcgt catgccaggc cgccactgat cctgcggctt
     8041 aagctgaatg gtcgtttcta ccatctccag cggagcagaa tcagtggcgg tttccgcttt
     8101 cccggtttgg ccaaataccc gcgccacttc cggtacgctc ataatcagct tgtcggtttt
     8161 ttgcagcata ctcgccgcct ccgctgcgga aatccccggc agcgtcgatg gcatatacaa
     8221 caagtcgcct tcattgatct gcggtaaaaa ttccccgcca actttattga gcggccagag
     8281 aaccgtcagc accgaaagcg ctgccaccaa cagcgtggtt ttcggccagt gcagtacttt
     8341 cagcaacagc ggatgataaa cacgaatcaa aaagcgattg agcgggttac tgctttccgg
     8401 cggaattttg ccacggatcc agtagcccat caggatcggg atcactacga tcgccagtag
     8461 cgccgcaccc gccatcgcat aggttttggt gaacgccagc gggccaaaca gacgcccttc
     8521 ctgcccttcc agggtgaaga tcgggataaa cgacagcgtg ataatcagca gactgataaa
     8581 cagcgccggc cccacttcaa cagatgcatc ggtaatcacc tgccagcgcg ttttattatc
     8641 caacgtggcg tcaggatgct ggtgctgcca ctcttccagc cgtttatgcg cattctcgat
     8701 catgacgata gcggcatcga ccatcgcccc gacggcaatc gcaatgccac ccaacgacat
     8761 aatattggcg ttcagtccct gaaagtgcat gacaataaaa gcaatacaca accccagcgg
     8821 caacgaaata atcgccacca gcgccgagcg cacatgccag agaaacagcg cacagactac
     8881 cgccaccaca ataaactctt ccagcaactt gccgctgagg ttgtcgatgg cgcgatcaat
     8941 gagctggctg cgatcgtatg ttgtaactat ctccacgcct tccggcagac tacttttcag
     9001 cgtttccagt ttgtccttca cggcggcgat tacttctcgg gcgtttttgc cggagcgcag
     9061 gatcaccacc ccgcccgcca cttcgccttc gccgttcagt tcggcaatgc cccggcgcat
     9121 ctccgggcca atctggacct tcgcaacatc acgcagataa acgggcacgc cattttcact
     9181 ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgc agatagccgc tggctcgcac
     9241 catatattcc gcttccgcca gttcgatcga cgaaccaccc gcttcctggt ttgaagcatc
     9301 cagcgcgctt tttacttcgg cgagactgat gccatattgc gccaggcgct gaggatcgat
     9361 aaccacctga tactctttca ccacaccgcc taccgacgcc acttccgcaa cgtcaggaat
     9421 ggttttcagc tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg caagatcgtg
     9481 cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg tggcatctgg
     9541 ccccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga ggtactccag
     9601 cacccgcgag cgcgcccagt acggatcggt gccatcttcg aaaatgacat acacataaga
     9661 gtcgccaaac tgcgagaaac cgcgcacagt cttcgcgcca ggcaccgaca acatggtggt
     9721 ggttagcgga taagtcacct gattttcaac gatttgcggt gcctgaccgg gatagctggt
     9781 tttaataatc acctgcacat cagagagatc cggcagtgca tccactggcg tgttaatgat
     9841 ggtccaggtg ccccagatac tcagaaacaa cgcgcccatc agcaccagaa aacggttcgc
     9901 caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc atgggtagca
     9961 ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc aatcaggaac
    10021 aggccgctgg aaaccacctt ttcaccttcc gccagaccag agcgtaacgc ggtgacgcct
    10081 tgcgatgcct ggaaaacggc aacgcgtttc ggtacaaagc gtccgtcggc atcaacggta
    10141 atcacccgct gttcactgcc ggtatcaatc agcgcctgtg acggaatgag cagcatcggt
    10201 tcgctggcgg tattgagttg caaccaggcg ttcatgcccg gttttagcga ctcgtcggcg
    10261 ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg catccacgcc gggtagcagg
    10321 gtccatttgc gaatggtgag tgttttatca ggtcgcgccg gaacggtgag cgtaaactgc
    10381 gaggcatctt tcaccagcca tgcgatggac tccgggatcg cggcagtgat ccacaccggg
    10441 tccataccct gaattttcgc gaccacgtta tctttggcga tattcattcc cgcgcgcaga
    10501 tcaaacgcgg tgatcacgcc atcaatgggt gctttgagcg taaagcgagt ctggattttt
    10561 tgcgtggcaa tcaggcggcg aatatccgcc tccggcattc ccgccagtcg cagtcgctca
    10621 agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc gcagcagtaa atactcactc
    10681 tgcgcttcca cccagtcggg aatggtcagg tcgagaagcg gtgtgccctt ttgtacttta
    10741 tcgcccacgg taagcggata caccttatcg ataaacccgg cagcgcgagc ctgcacaatg
    10801 gcatactgat actcgttgta actgacattc gccgggaaac tctgggcaaa agtcagcggc
    10861 ccgcgcgtta cggtcgccgt tttcaccccc agattctgcg tctgggtcgg gtcaatgcgt
    10921 acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat atccataaac
    10981 ggcgatttac ctggtttatc gaaccgcgta ttgggataca tcgggtcgta ccagaataag
    11041 attttacgtt ctgcggtcga cgttttttct gcgggcggtt ccgcctttgc aaaccaggta
    11101 aaacctgccg cagaaataat accgcccgcg atcatgctgc cgataataag cgcgattttt
    11161 ttcatctcat taaacctggg ttactggctg actttaatat cctgtaataa agaaaggttg
    11221 ccctgctgga caaaattaaa cgccactttg tcgccggttt taatttcact cattttcgtc
    11281 tgcggggtga tggtaaagcg catggtcatc tccggccagt tcaccgcggt aattggatcg
    11341 tggtggatga tgattttttt gctttccaga tcaatggttt ttaccacacc ggtggcgcta
    11401 atgacctgtg gttgtgcctc gctcatggtt tcatgatgat gttcgttagc ctgggcatta
    11461 aagccaataa cggtaaacag actaaacatt gcgacttgca gtgctttttt catttattct
    11521 ctcctggagt taaaaattat tgctgccaac cgccgcctaa ggcggtatat aaagaaattt
    11581 cattaacctg acgggcataa ttcaggtcaa gtaaagtttg tcgggttgca aataaagaac
    11641 gctcagcatc cagcacttcc agataactta ccgcgccgtg ctgatataat gcccgcgccc
    11701 gttgcaaagt aatttgcagc gacgccagat aacgctgctg ggcgctaatt tgatcgttca
    11761 ggctttgacg taatgccagc gcatctgcca cttctttaaa ggcgttctgg attttctgtt
    11821 cataattcac caccgactgc tgctggcgaa tttcggcgat atccagattg gcctggttac
    11881 gtccggcatt aaaaatgggg atctcaattt tgggtataaa attccacatc ccgctgctgg
    11941 cgttaaataa tgacgacaga tcgctgctgg cggttgatat tccgctggtc aggctgatag
    12001 acgggaaaaa tgccgcgcgt gcagcaccaa tattggcatt agccgccatt aacgcatgtt
    12061 cagcttccat aatatccggg cgctgcaata agatttgcga cgacaagccc gccggtaatt
    12121 taacgctttg caggctgtcg ctgtttactg tctgcgcttg cggcagcttg ccgtagcttc
    12181 ccaataacag ttgcaatgca ttattcgcct gcgccagttc cccctgacgt ttagcgatgt
    12241 cgctgcgggt actttcaatc accccgcgag cctgttccag cgccagaaca ttactgctac
    12301 ctgtcaacag ttgtttttcg acaaacgcat atgactgctg ataattacgc agcgtttctt
    12361 cggctatttg caattgcgca tacgccagct gctgattgaa atagctttgc gcgacattag
    12421 aaaccagcaa aatatgcacc gcgcgctgag cttcctcagt ggctaaataa ttttggcgct
    12481 cggcttcgct catgttcttt aagcgcccga aaaaatcgag atcaaagctg gcgttaaggc
    12541 cagtcgagaa ctcccgcgtc gtggctgaat cgcctttaag attgttgctc cagctgccgc
    12601 tgccctcgcc attgagctgt gggtagcggt cggcatcggt cagacgatat tgcgcccgcg
    12661 cttcctgcac tttcagcgtc gccatgcgca aatcccggtt attcaccagc gcctcgctaa
    12721 tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca gcccgcgttc tggtagttat
    12781 ctgctgcgtt aaccaggccg ttctggttga gtgagaactg ctgcggcacg ggcattgccg
    12841 ggcgctgata atccggtgcc agtgaacaac cggttagcgc aagggccaca caaaatggca
    12901 gaagtttaca aggagacata ggctcataat ttctggtgat tttatgccgc caactttact
    12961 cgccaggctc tgattttccg gtgacaggaa aatgacaaaa ttgtcatttt gccaataagc
    13021 gattgccatc tgatcccgct actctagaat tgcccgggca acatgcggag gaaatatgaa
    13081 actgttgatt gtcgaagatg aaaagaaaac cggagaatac ttgaccaaag ggttaaccga
    13141 agccggtttt gtggtcgatt tggccgacaa cgggctgaat ggctaccatc tggcgatgac
    13201 cggtgattat gatctgataa tcctcgatat tatgctgccg gacgtgaacg gctgggatat
    13261 cgtgcgcatg ttgcgttccg ccaataaggg gatgccgatt ctgttgctta ccgcgcttgg
    13321 caccattgaa catcgcgtca aggggctgga gttgggggcg gatgactatc tggtgaagcc
    13381 gttcgctttt gctgaactgc tggcgcgagt gcgcactcta ctgcggcgcg gggcggcggt
    13441 gattatcgaa agtcagtttc aggttgccga tctgatggtc gatctcgtca gccgcaaagt
    13501 cacccgcagt ggcacgcgca tcactctgac cagtaaagaa tttactctgc tggagttttt
    13561 ccttcgccat cagggcgaag tactgccccg ctcacttatt gcctcacagg tgtgggacat
    13621 gaatttcgac agcgacacta acgccattga tgtggcggtg aagcggctgc gtggcaaaat
    13681 cgacaacgac tttgagccga agctgattca aaccgtgcgc ggcgtgggtt acatgcttga
    13741 ggtgccggat ggtcagtaag ccgtttcagc gcccgttttc gctggcaacc cgcctgacct
    13801 tttttattag cctggccacc atcgcggcgt ttttcgcctt tgcatggatc atgatccact
    13861 cagtaaaagt gcattttgcc gagcaggata ttaatgattt aaaagagatt agcgccactc
    13921 ttgaacgggt acttaatcac cctgacgaaa cgcaagccca acgcttaatg acgctggaag
    13981 atatcgtcag tggttattcc aatgtgttga tttccctggc agatagccac ggtaaaacgg
    14041 tgtatcactc ccccggtgcg ccggatatcc gcgagtttac gcgtgacgcc atacccgata
    14101 aagacacgcg gggcggtgag gtgtatctcc tttccggccc gacgatgatg atgccgggcc
    14161 acggtcacgg gcatatggaa cacagcaact ggcggatgat tagcttgccg gttggcccgt
    14221 tggtggacgg caaaccgatt tatacgctct acattgcgct ttcgatcgat tttcatcttc
    14281 attacataaa tgatttgatg aataaactta ttatgaccgc atcgatcatc agcatcctga
    14341 tcgtctttat cgtgctgttg gcggtacata aaggtcacgc gccgatccgc agcgtcagcc
    14401 gtcaaatcca gaacattacc tcgaaagatc tcgacgttcg ccttgacccg cagaccgtgc
    14461 ccattgagct ggaacagctg gtactgtcgt tcaaccatat gatcgagcgt attgaggatg
    14521 tctttacccg ccagtccaat ttctcagcgg acatcgccca cgaaattcgt acaccaatta
    14581 cgaatctcat aacgcaaacg gaaatcgccc tcagccagtc acgcagccag aaggagctgg
    14641 aagatgtgct ctactctaat ctcgaagagc tgacgcgaat ggcgaaaatg gtcagcgata
    14701 tgctgtttct cgcccaggcc gataacaacc agttaatccc cgaaaagaaa atgctcaacc
    14761 tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc attagcggaa gatcgcggcg
    14821 tggagttgcg gtttgttggt gacgagtgtc aggttgcggg cgatccgctg atgctgcgtc
    14881 gggcgttaag caacctgctt tcgaacgccc tgcgttatac gccacccgga gaggcaattg
    14941 ttgtgcgctg ccaaacggtc gatcaccagg tgcaagttac cgtcgaaaac cccggtacgc
    15001 ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt ctatcgcgtt gacccctccc
    15061 gtcagcgaaa aggcgaaggt agcggtattg gcctggcgat agtgaaatcg attgttgtcg
    15121 cgcataaagg cacggttgca gtaacgtcag atgctcgggg gacaaggttt atgatcaaac
    15181 taccggagaa aggttaatta gttagttcaa actcaaagag tgatgtatcg ccttctgtta
    15241 ttatttatca ataaaacagg gaagacctta ttggtcgtct gaaatcgaag gttattcatt
    15301 ttcg
//
LOCUS       DAZUCC010000120        15287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZUCC010000120 DAZUCC010000000
VERSION     DAZUCC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.120"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..617
                     /gene="yceJ"
                     /locus_tag="ACHY09_003134"
     CDS             51..617
                     /gene="yceJ"
                     /locus_tag="ACHY09_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFS3886075.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHQAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            621..1196
                     /gene="yceI"
                     /locus_tag="ACHY09_003135"
     CDS             621..1196
                     /gene="yceI"
                     /locus_tag="ACHY09_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFS3886076.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1238..2290)
                     /gene="trhO"
                     /locus_tag="ACHY09_003136"
     CDS             complement(1238..2290)
                     /gene="trhO"
                     /locus_tag="ACHY09_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS3886077.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2515..3435
                     /gene="lpxL"
                     /locus_tag="ACHY09_003137"
     CDS             2515..3435
                     /gene="lpxL"
                     /locus_tag="ACHY09_003137"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFS3886078.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKTLKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3607..4833
                     /gene="mdtG"
                     /locus_tag="ACHY09_003138"
     CDS             3607..4833
                     /gene="mdtG"
                     /locus_tag="ACHY09_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFS3886079.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4916..5290
                     /gene="msyB"
                     /locus_tag="ACHY09_003139"
     CDS             4916..5290
                     /gene="msyB"
                     /locus_tag="ACHY09_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFS3886080.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5291..5518)
                     /gene="yceK"
                     /locus_tag="ACHY09_003140"
     CDS             complement(5291..5518)
                     /gene="yceK"
                     /locus_tag="ACHY09_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFS3886081.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5690..8233)
                     /gene="mdoH"
                     /locus_tag="ACHY09_003141"
     CDS             complement(5690..8233)
                     /gene="mdoH"
                     /locus_tag="ACHY09_003141"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFS3886082.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8226..9761)
                     /gene="mdoG"
                     /locus_tag="ACHY09_003142"
     CDS             complement(8226..9761)
                     /gene="mdoG"
                     /locus_tag="ACHY09_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFS3886083.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10155..11312
                     /gene="mdoC"
                     /locus_tag="ACHY09_003143"
     CDS             10155..11312
                     /gene="mdoC"
                     /locus_tag="ACHY09_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFS3886084.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11320..12801)
                     /gene="clsC"
                     /locus_tag="ACHY09_003144"
     CDS             complement(11320..12801)
                     /gene="clsC"
                     /locus_tag="ACHY09_003144"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFS3886085.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGIFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12743..13276)
                     /gene="ymdB"
                     /locus_tag="ACHY09_003145"
     CDS             complement(12743..13276)
                     /gene="ymdB"
                     /locus_tag="ACHY09_003145"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFS3886086.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13371..13682)
                     /gene="ymdA"
                     /locus_tag="ACHY09_003146"
     CDS             complement(13371..13682)
                     /gene="ymdA"
                     /locus_tag="ACHY09_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFS3886087.1"
                     /translation="MFRPFLDSLMLGSMFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13803..14135)
                     /gene="csgC"
                     /locus_tag="ACHY09_003147"
     CDS             complement(13803..14135)
                     /gene="csgC"
                     /locus_tag="ACHY09_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFS3886088.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14194..14649)
                     /gene="csgA"
                     /locus_tag="ACHY09_003148"
     CDS             complement(14194..14649)
                     /gene="csgA"
                     /locus_tag="ACHY09_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFS3886089.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14690..15145)
                     /gene="csgB"
                     /locus_tag="ACHY09_003149"
     CDS             complement(14690..15145)
                     /gene="csgB"
                     /locus_tag="ACHY09_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFS3886090.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3792 a         3929 c         3653 g         3913 t
ORIGIN      
        1 tctccgacac gccattttct ttctctcgca aagtcgtttt ggatgcactt atgtcattca
       61 caaatacccc tgaacgctat ggtgttatat ctgcggcctt ccattggcta agcgcaatta
      121 ttgtctacgg catgtttgct ttaggtctgt ggatggtcac gctcagttat tacgatggct
      181 ggtatcacca ggcacccgaa ctgcataaaa gcatcggtat tttgttaatg atggggctgg
      241 ttattcgcgt tctgtggcgc gtcatatctc caccgccggg tccgctgcag agttattcgc
      301 ccatgactcg tcttggcgca aaggctggac atctcgcatt gtacttattg ctgttcgcca
      361 tcggcatcag cggctatctg atctcaactg ccgatggtaa accgatcagc gtttttggct
      421 ggtttgacgt ccccgcgacc ctcgccgacg ctggcgcaca ggctgacttt gccggtgccc
      481 tgcatttttg gctcgcgtgg agcgtcgtag tactgtccgt tatgcacgga tttatggccc
      541 tgaagcatca tttcatcgat aaagacgaca ctctgaagcg catgctggga aagtcgtcat
      601 ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg tttaaccttc gcttccctga
      661 tgttctctgc cggttcagcg gttgccgccg attacaaaat tgacaaagaa ggtcagcacg
      721 cctttgttaa cttccgcata cagcaccttg gctatagctg gttatacggc acctttaaag
      781 atttcgacgg tacttttacc tttgatgaaa aaaatccagc tgccgataaa gtgaatgtga
      841 caattaacac caccagcgtc gatactaatc acgccgaacg cgataaacat cttcgcagtg
      901 cagatttcct caatactgca aaatatccac aggcaacatt cacctccacc agcgtgaaga
      961 aagacggtga cgaactggat attaccggcg atctgacgct gaatggcgta accaaacccg
     1021 tcacgctgga agcgaaatta attggtcagg gcgacgaccc atggggtggt aaacgtgcag
     1081 gcttcgaggc cgaaggcaaa attaagctca aggacttcaa tatcaagaca gatttaggtc
     1141 cagcttctca ggaagtggat ctgattattt cagtggaagg cgtacagcag aagtaagagt
     1201 gaaatgttgc cggatggcac gcatccggca atgatattta ttctgttgga tcaggaatgc
     1261 ccagtgttgt attcagacgt ccacgagact tattaaagat cttattgcca ttttcacgtc
     1321 ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct ttcttcgcag caaatttcac
     1381 tacaacaacc tttgtatttt tctgcacata ctggacactg aataaacagc aggtggcagc
     1441 catcattttt acagttggta tggctgtcgc acggcgcacc gcactggtgg caatgcgcga
     1501 taatctcatc agatatacgt tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa
     1561 aacgtaccgg taaaccctgc tcgcgcgcct tgcgggcgta ttcaataatt ccgccctcga
     1621 tatgccagac tttattgaat ccgttatgtt tcatccaggc actggccttt tcacaacgaa
     1681 tgccgccggt gcagtacata acgatttttt tatctttatg tgcctgcatc atctctactg
     1741 cttttggcag ctgctcacgg aaggtatctg ccggaatttc cagcgcgttt tcaaagtgcc
     1801 ccacttcata ctcatagtgg ttacgcatgt cgataaacaa tgcatcggga tcgtcaagca
     1861 tggcgttcac ttccgccgct tgcagatact cacccacatt gctggcatca aagtgaggat
     1921 cgtcaatacc gtcggcaacg atacgatcgc ggaccttcat gcgcagtacc cagaaggatt
     1981 tcccgtcatc atccaacgcg atattcaggc gtaagccctc taaagccggg tcgaaggcat
     2041 aaagctgcgc gcgaaatgtt tcaacattgc tcgcaggtac gctgatttgc gcgttaatgc
     2101 cctcatgcgc cagatacact cgcccaaaaa catccagcgc ggtaaacagc tgatataaag
     2161 cgtcacgggt cgccttagga tcggcgatgt ggaaatactt gtaaaacgaa atggtggttc
     2221 gcggttcgct ctcagccaac attttggctt ttaacgcgtc gttggaaatg cggttgtgta
     2281 acactggcat ggtgtacggt tcctgcgaga tgggaaagta aaaatccgcg gcatgatata
     2341 gcaattatcg ataattaaca tccacacatt ttacgctaca tttgcgcatt aaaaattatt
     2401 tgttatttac aagcgcggca atttcgccca gtcttcagcc acaaattttg gttgcgggcg
     2461 aaaaaatgcg acaatacata caattgcccg tataggttga aaaacaggat tgatatgacg
     2521 aatctaccca agttctccac cgcactgctt catccgcgtt attggttaac ctggttgggt
     2581 attggcgtac tttggttagt cgtgcaattg ccctacccgg ttatctaccg cctcggttgt
     2641 ggattaggaa aacttgcgtt acgttttatg aaacgacgcg caaaaattgt gcatcgcaac
     2701 ctggaactgt gcttcccgga aatgagcgaa caagaacgcc gtaaaatggt ggtgaagaat
     2761 ttcgaatccg ttggcatggg cctgatggaa accggcatgg cgtggttctg gccggaccgc
     2821 agaatcgccc gctggacgga agtgatcggc atggaacaca ttcgtgacgt gcaggcgcaa
     2881 aaacgcggca tcctgttagt tggcatccat tttctgacac tggagcttgg tgcgcggcag
     2941 tttggtatgc aggaaccggg gattggcgtt taccgcccga acgataatcc actgattgac
     3001 tggctacaaa cctggggccg tttgcgctca aataaatcga tgctcgaccg caaagattta
     3061 aaaggcatga ttaaaaccct gaaaaaaggc gaagtggtct ggtacgcacc ggatcatgat
     3121 tacggcccgc gctcaagcgt ttttgtcccg ttgtttgccg ttgagcaggc tgcgaccacg
     3181 accggaacct ggatgctggc acggatgtcc ggcgcatgtc tggtgccctt cgttccacgc
     3241 cgtaagccag atggcaaagg gtatcagttg attatgctgc cgccagagtg ttctccgccg
     3301 ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag tggtcgaaaa atgcatcatg
     3361 atggcaccag agcagtatat gtggttacac cgtcgcttta aaacacgtcc ggaaggcgtt
     3421 ccttcacgct attaaatctc ccaagccgga tgcttgagaa tagcatccgg cattaccaca
     3481 gcaaatcccc ctgatttagc gataaaagct ctctggattg cgccccctgg aagtcgggcg
     3541 cataattagt gtgcttatct tttcttctta tgttcaccgc gcctggcgca ccaacagcgg
     3601 attgctatgt caccctgtga aaatgacacc cctataaact ggaaacgaaa cctgatcgtc
     3661 gcctggctag gctgttttct tacgggtgcc gccttcagcc tggtaatgcc cttcttaccc
     3721 ctctacgttg agcagcttgg cgttacgggc cactccgcct tgaatatgtg gtccggtatt
     3781 gtcttcagca ttacattttt attttcggcc atcgcctcac cgttttgggg tggactcgcc
     3841 gaccgtaaag gccgaaaact catgctatta cgctctgccc tcggcatggg catcgtgatg
     3901 gtgttgatgg gactggcaca aaatatctgg cagtttttga tcctgcgggc gcttcttggg
     3961 ttacttggcg gatttgtccc caacgctaat gctcttatcg ccacacaagt accgcgtaat
     4021 aaaagcggct gggcgctggg tacgctctcc acaggcggcg ttagtggtgc gttgctcggc
     4081 ccaatggctg gcggcctgct cgccgatagc tacggcttac gtccggtatt ctttattacc
     4141 gccagtgtgc tcatactctg ctttttcgtc accctgttct gcatcagaga aaaattccag
     4201 ccggtcagca aaaaagagat gctgcacatg cgggaagtgg tgacatcact taaaaacccg
     4261 aaactggtac tcagcctgtt tgtcaccacg ttaatcatcc aggtggcgac aggctcaatt
     4321 gcccccattc tgacgctgta tgtccgcgaa ctggcgggta acgtcagtaa cgtcgccttt
     4381 atcagtggca tgatcgcctc ggtgccaggc gtggcggctc tgctaagcgc accacgactc
     4441 ggcaaacttg gcgatcgaat cggacccgaa aagatcctga ttacagcgct gatcttttct
     4501 gtactgctgt tgatcccaat gtcttacgtt cagacgccat tgcaacttgg gattttacgt
     4561 tttttgctcg gtgccgccga tggtgcacta ctccccgccg tacagacact gttggtttac
     4621 aactcgagca accagatcgc cgggcgtatc ttcagctata accaatcgtt tcgtgatatt
     4681 ggcaacgtta ccggaccatt gatgggagca gcgatttcag cgaactacgg tttcagagcg
     4741 gtatttctcg tcaccgctgg cgtcgtgtta ttcaacgcag tctattcatg gaacagtcta
     4801 cgtcgtcgtc gaatacccca ggtatcgaac tgatttttcg cctttcatac ttgcaaaagc
     4861 ggagaatcag ctatcctttt ccctgaaacc tcatcaactc aaagggagaa tcgtgatgac
     4921 catgtacgca acgcttgaag aagccattga cgctgcacgc gaagaatttc ttgcagacaa
     4981 ccccggcatc gacgccgaag atgcgaatgt gcaacagttc aatgcccaaa aatacgtttt
     5041 gcaggacggc gacatcatgt ggcaagttga gttttttgcc gacgaagggg aagaaggtga
     5101 atgtttacct atgcttagcg gtgaagccgc gcaaagtgtt tttgatggcg actatgatga
     5161 gatagagata cgccaggagt ggcaggaaga gaatacatta catgaatggg acgaggggga
     5221 atttcagctt gagccaccgc tggataccga ggaaggacgc gcagcagctg atgagtggga
     5281 tgaacgttaa tcactcatac gggccatgat gaatgtcgat cggcagcagt aaagtatcga
     5341 aaaccaggga gaatggcaga tcaaggatcg tgacgtaacg ccaggcggag tcacgcacgt
     5401 cccattgcac accgggataa tattggttgc catgcccctg ccccggtata gtgcgactaa
     5461 tgatgctgcc acagccactc agcaaggtca ccattatgct catcacaatt aaacgcattt
     5521 gatacctctt ccgtaaaaaa taccggcata acgccggtat ttttattggc tcgttgtttt
     5581 tgccggatgc ggcgtgaacg ctttacccga cctacaaaat cttgcaaaat caataaattg
     5641 caggaacgat gtaggcctga taagcgtagc gcatcaggca actacgtttt tattgcgaag
     5701 ccgcatccgg tttacgcaat gccagtggat tgagctttat cccttcgtaa taactcaccc
     5761 atgaagaata tctctccggg gaattccaga cgcggaaatg cagacgggcc atcgtcaccg
     5821 gatcgcttag cagcaccagg cgacgatcgc gattcagctt ctctggcgtc tcgttcagcg
     5881 cctgttcaac gtggcggtca cgggcgattt ccagcacctt gctggcgcgg tgacgcgcgg
     5941 tcgccattgc ggttgccaga gcgttaaatg acgggttaaa caccgcgtgc ataaaaccat
     6001 catcaaggga gcgttgacga ttcatctcaa ggaaccgatc ggtatcaacc agcacctgcg
     6061 gcggtgaata ctcttccggg atcaggaaca gtttccagcg tttggttcgc agaccaacgg
     6121 tggcacggct ggaaatcacc gaaacaaacg gtgacaggat caacgagaag acaatcggtg
     6181 ccagccagaa cagaaaacgc agatccaacc acgccatccc aacagcccac actaaaccca
     6241 acagcagctg tgagccgtgg cgtttgaatg cttcgcccca ggaagtggag tcatcatcac
     6301 gctgcggtga attccacacc acttcccagc caaggaacgc gctgacgacg aagactgtat
     6361 ggaacaacat gcgtaccgga gccagcaaca cggaaaacag cacttccagc agcaacgata
     6421 atgtaacgcg ccagaagccg ccgtattctt tcgttccttt gcaccagata agcaaaatgc
     6481 tcaatagctt cggcaggaac aacagcacca tggtcgaagc aaaaagtgca atcgccagct
     6541 caggacgcca ctgcggccat accgggaaca actgccgtgg ttgcaggaag tattgcggtt
     6601 cggtcaacgc atgtactacc tgcaatgcag tagagagcgc gaggaacata aaccacagcg
     6661 gagcggagag ataagacatc acgcccgtca ggaacaccgc acggtgaacc gggtgcatac
     6721 ccttcaccag gaacagacgg aagttcatca ggttaccgtg gcaccagcgg cggtcacgtt
     6781 ttagctcatc aagcaagtta ggcggcaatt cttcataaga acccgggaga tcgtaagcaa
     6841 tccacacccc ccaacctgca cggcgcatca acgccgcttc tacgaagtca tgtgacagga
     6901 ttgaaccggc aaaagaacct tcgcccggca gcggagccag tgcacaatgc tcgataaacg
     6961 gtttcacgcg gataatcgcg ttatgccccc agtagtgcga ctcgccaagt tgccagaagt
     7021 gcaaaccggc tgtaaacagt ggcccataca cgcgggtcgc gaactgctga cagcgcgcat
     7081 acagcgtatc cataccggac gctttcggcg acgactgaat gatcccggcg ttcgggttgg
     7141 cttccatcaa gcgcaccagg ccgcataaac aatcaccggt cattaccgag tcagcatcca
     7201 gcaccaccat gtagctgtac tggctgcccc agcgacggca gaagtcatcg atattaccgc
     7261 ttttacgctt cacgcgacgg cggcggcggc gatagaaaat ctgaccttcg ccaccgactt
     7321 cagcgataag ctccatccag gctttttgct ctgcgacgca gatatccggg ttataactgt
     7381 cactcagaat gtagacatca aagtgtttgg cattcccggt ggcttttact gattcccacg
     7441 ttgcacgcaa gccagcaaaa acacggttca cgtcttcgtt acagataggc atgatcaacg
     7501 ccgtgcgatg ctccgggttt aatggttcat cgccaactgt tgacgcggat atactgtatt
     7561 tatcgcgacc aataagcagt tgcaggaagc ccatcaacgc cgtccagaat ccggcggaca
     7621 cccaacagaa cagtaccgca aagaggatca ggataccggt ttgcagcata taaggcagaa
     7681 gctgcataaa agaaacccac acatcctgac caaccatatc cataggatta atcagcgccc
     7741 acccctgata aggaagaatg gtcttcatat accaggtcgc gacaacagtt tgcgcgagcg
     7801 tcaggatcaa cagaatgtaa cggcggatgg taccgacggt acgccacttt tgctcgctct
     7861 cctgctcttc tttggtcaaa cgagccagat agcgcggcgt cacatcgcgt ccacgcaggc
     7921 gatcccagaa acggcctacc gggttggtac gccacgggtc gggaaacatc gaggagcgtt
     7981 ttgcttctgg catcgcctta agctgatcgc gcccttcgtc atctttaatt aactgtccat
     8041 cagcaagtga atctggccag gcttgttcca gacgcgcctt taccgagcct tgcggggagt
     8101 catcctcccg cgcccaggtg cggtgttcgg catccagcgc ctgatgaacg gcgcggatat
     8161 cagtcttcgg caatgccgct ttctcgcttg cggcgatggg cattgcgtca atgtactcag
     8221 ttgtcttatt cattggcagg taactggtag ctccaggttt cactcaacgt ctgatcggca
     8281 ttcaccagcg cagcacgcat ttcagtggtt ttcttggcat ctttcacttt cacacgcatc
     8341 accagacgcc agcctttggt aaccgggtta tagcgcaccg tgctttcaac tatctcacca
     8401 ttatcaccaa tgctggtttg cgctgtgacc ggggtatcct ctggcagttt tttcatctct
     8461 gcgccggtaa aatcgaccac aaaggcgata gtaccgtcag gttggcgaat caggttcgac
     8521 tgcttcacat cccccgttga acgacgcgtt tgttgcaccc atgcgttatc tggcgcatgc
     8581 agtttgtctt catcacggct gaaggtgatg gtgtatttaa agttcatctc tttacccggc
     8641 tccggcagct gatccggcgt ccagtaagcg acgatgttat cgttggtttc atcgttggtt
     8701 ggaatttcca ccagctcaac gctgcctttg ccccactccc ctttcggagt cacccatgcg
     8761 cttggacgga gatcgtaacg atcatcgaga tcttcaaagc gggagaaatc acgaccgcgc
     8821 tgcaacagac cgaagccttg cgggttttcc atcgagaagc tgctgaccgc taaatgtttc
     8881 gggttattca acggacgcca gatccattcg ccattaccag catggataga gagaccgtta
     8941 gagtcgtgca actccggacg atagttattt gcaggcgacg gttggttcgg cccaaacagg
     9001 aacatactgg ttaacggtgc aacccccagt ttgccgactt tatcgcgcag atagattttc
     9061 gactgcacat ccacaaccgt gtcacgccct ggcataacta cgaatttgta agcacctgtc
     9121 gcgcgcggcg agtcaagcaa tgcataaatg gttaaacgtt tatcagtcgg ttttggacgc
     9181 tcgatccaga actctttgaa gcgtggaaat tcttcacccg atggcaaggc ggtatcaatt
     9241 gccaggccgc gtgcagaaag gccataaacc tgacctgcac caatcacgcg gaaatagctg
     9301 gccccgagca tgctgacgat ttcatcgttt ttatctttgc tgttgatcgg gtaaagcact
     9361 ttaaaaccgg caaaaccaag gtcttttacc gtgtctttgt catgctgaac atcgccgaaa
     9421 gtgaaataat ccgggctgta tttgattcgt ttgactgcgg tggcagtcac ttcatttatt
     9481 ttgaccgggg tatcgaagta cataccctga tggtagaact cgagtttgaa tggggtcttc
     9541 agattgttcc agtacgcttt gtcatgatta aactggatct gctgatagtc cgcgtatttc
     9601 atgtcgcgga aaacggaggg caagttgctt ttaggcgcct catagccttt cccggctaag
     9661 gattgagctt gctttgcgac atcatcaata ctgaaagccc agcttgaaga tgtatacagg
     9721 gttaacatta ctgcagcact caaccaacgc attttcatca tttgtagttt atgtttcata
     9781 attagtaagc acttccccct ttgtgtgctt atatcgatcc gatctatttt aatggataac
     9841 cagccattcc gacaacagaa tcccggtatc gttcagcacg ctggctcatg gattaagcac
     9901 ctgattgcaa ccgttttcac tttccgtgct tctcctaaag acagaagcat cgacttcgtg
     9961 tagggtttgc cagcaaattt atttatcaac cgtcttacag acgcttccat tctggttagt
    10021 ggtagcgatg atgctatgca taaaaacctt gaagcctgcg cctggcgcgg tcaggtcggt
    10081 gtcgctatgg caacaagcct aaaggcataa cccgacaggg ataagacgaa aaatccgaga
    10141 ttagttaacc atatatgaat ccagtacccg cgcaacgtga atatttcctc gactccatcc
    10201 gcgcctggct gatgttgtta gggatccctt ttcatatttc tttaatctat tcgagccata
    10261 catggcatgt gaatagcgcc gaaccgtcat tgtggctgac cctttttaat gacttcatcc
    10321 actcgttccg catgcaggta tttttcgtta tatccgggta cttttcctac atgctttttt
    10381 tacgctatcc cttgaaaaaa tggtggaaag tacgtgtcga acgtgtaggt atcccgatgt
    10441 taacagccat ccccctactg acattaccgc aatttattat gctgcaatac gtcaaaggga
    10501 aagcggaaag ttggcctggg ctatcattgt atgacaaata taatacgttg gcctgggaat
    10561 taatatcaca cctgtggttt ttactggtgt tagtagtcat gacgacgctg tgcgtatgga
    10621 tatttaaacg catcagaaat aatttagaaa attctgataa aacgaataaa aaattctcga
    10681 tggtaaaact atcggtgatt tttttgtgcc tcggcatcgg ttatgcggta ataagaagaa
    10741 cgatttttat tgtgtatccg cccattctga gtaatggcat gttcaatttt attgtcatgc
    10801 aaacgctatt ttatttgccg ttctttatcc tcggcgcact ggctttcatt ttccctcatc
    10861 ttaaagcctt gtttaccacg ccgtctcgtg gctgtaccct tgcagcagca ttggcatttg
    10921 tcgcttattt actcaaccag cgctatggca gtggcgatgc ctggatgtac gaaaccgagt
    10981 cggtgatcac catggtcctc ggtctgtgga tggtgaatgt ggtcttctcc tttggccacc
    11041 gtttgcttaa cttccagtca gcgcgggtga cttattttgt taacgcatcg ctgtttatct
    11101 atctggttca ccacccgtta acgctgtttt tcggcgcata cattacaccg cacatcacct
    11161 ccaactggct tggttttctc tgtggcctga tattcgtagt agggattgcg ataattctgt
    11221 atgaaattca tttacgcatc ccgttactga agtttttgtt ctctggtaaa ccggttgtca
    11281 agcgtgagaa cgataaagca ccagcccgtt aagccacgct tataataacc attccacggg
    11341 caatatcgac gccagtctga ccataacccg cttccagaaa ctggtggcgg gttctttttt
    11401 gagaataatc tctttctttg catgacgatc aacccagttg atccgtcccc acctgtccag
    11461 acggagctgc caggccgcat catactggct ctgaataaag cgtttatcaa ttaactgtgc
    11521 cagcgtttcg ctctctatca cgaagcccat ttcagtattg agcaatgttg aacgcggatc
    11581 gaaattgaaa gagccgataa acaccgtttt accatcgatg ctaaaggttt tagcatgcaa
    11641 gctggcaccg gaattaccgg ttatgccgcg atcgtgtaac gtactacttt gttcacgcgt
    11701 cggcttgagt tcatataatt ccacgccata gcggagcaat tttttgcgcc agcgcgcgta
    11761 tccggcatgg acgacagcaa catcgttagc ggcaagagaa ttggttagga tcgcaatctt
    11821 tacccctttt ctcaccatcc gtaagagttg cgccacacct gcgcgtgtcg gtacaaaata
    11881 ggaagagata atatcgatgc gttcactggg tgagcccatg atatcgaaca ggcgctgcgg
    11941 taacagtgaa tgccgttttg ccttgccttc ccctttcgcc ggatcatcac ttaataaacg
    12001 tgttttcgcc cagataagcg gcaacgttcc atcaaccaga tgatttataa acgggctgga
    12061 ttccattttg cgtaaataac gatgcgtcat ggcatcgttg tgccaggatg ctggtaactc
    12121 gatgcgatcc gccatttcac cttccgggac atccagcacc tgctgtaggg gtgaaaccga
    12181 tttgcaatac cagtagcggg cgaaatcatc ggcaacgtcc tctacaactg gtcctattgc
    12241 catgacatct aaatccgaaa aaagtggctc ttcccctgcc ccaaaatagg catcaccaat
    12301 atttcgcccc cctaccagcg tcaccacgcc atcgacagtg aaacttttat tgtgcatacg
    12361 gcgattgaga cgggaaaagt cggtgatata accaagcgga cgtagcaagc gaaacgagaa
    12421 aggattaaaa agccggactt caatgcgggg atgactgtca agcaagcgta aaatgtcgtc
    12481 aagtccgggc gtattgttgt cgtccagcaa caaacggacg cgaacgccac gctttgctgc
    12541 ggctaacagg gcggaaaaga gtaaccgacc cgacatatcg tcctgccaga tgtaatactg
    12601 aacatccagc gtatgctctg ccatttcggc cagacgatac cgggcggcaa acgcatccag
    12661 acttttctcc agaggaaaaa tgccacactg accgggatgt tgcgaacaca gtggcagcac
    12721 cgcactcgcc agccggggca aatcattcat ctccttgttg agtaaggagt ctttcgtaga
    12781 ggtgggcgtt ttcttcatca taacagacaa agtatacctg ttcgggtaaa gcgtgacggg
    12841 taataaattc tgaaacggtt tttactgcga tttcagccgc tgccgcacga gggtaaccat
    12901 aaactccagt actgattgca ggaaaagcca ctgacgtata gctgtttgct gccaccagtc
    12961 gcaggctatt gagataggca tcctgcaaaa gctggtcttc gttttgttca ccaccacgcc
    13021 agactggccc gacggtgtgc actacggctt tcgcgggaag atcgcctgca agcgtaataa
    13081 cggcatgccc cgtagggcaa tcgccctgct gctgcctgac ttttaaacag gcatccagca
    13141 gggccggacc cgctgcgcga tgaatggccc catcgacgcc gccgcctccc attaatgacg
    13201 gattagccgc attcacaatc acatcaacgg ccagtttggt aatatcaccc tgcacaacat
    13261 gaatacgcgt tttcataacg cctccttaca ccttcttgtt tcttaagtgt acagcaggct
    13321 ttttggttaa atcgcatttt tctgctcagc aacgtgagtt gttcagaaac ttagttatat
    13381 tcgatgttca ttacagccag ctttttctgt ttatcaagat actgcacttt tactgaggct
    13441 atttgcgcgt ttttgacatt gccgctcatt aacgctttgc tggaataggt tctgcccgga
    13501 acagagccat tctgtgggca gttcatttct acgggtgatg actgggtgct gacgtcacat
    13561 gccggttcca caatttggcc ataaaaatgg atcacgcccc cttgcgcggt gcttcctgcg
    13621 atggcaataa aaggaaaaaa catactgccg agcattagag agtctaaaaa aggtctgaac
    13681 atttattcta tttcctcaat gattagtcat ccttgagggt tgtgttatcc atactttcag
    13741 gttggctgat ccgcagcaga ccattctctc cagattcatc ttatgctcga tatttcaaca
    13801 aattaagact tttctgaaga gggcggccat tgttgtgata aatgaagtga ctgtccatca
    13861 gaaacagtaa caactatttt cacccgatcg tccggggaaa tatttaaact caacttcgtc
    13921 aaagcaatgg gttgattagc aggcaatgag agggtctttt cttgcttcgt ctgactttgc
    13981 cctgaactgc cttcgcgcag ggacaatatt tgtactctgc acagacaaga ttgagtaaga
    14041 gtgacttcag gaataatggt gtacatatcc ccttgctggg tcgtattaaa ggttatctga
    14101 ctggaaagtg ccgcaaggag taataacgca ttcatattct tctcccgaaa aaaaacaggg
    14161 cttgcgccct gtttctttaa tacagaggat gtattagtac tgatgagcgg tcgcgttgtt
    14221 accaaagcca acctgagtga cgttgacgct ggagttagat gcagtctggt caacagcagc
    14281 accgttacca ccaccgaact gtttaacagt catagtagaa tctttaccat tccactgatc
    14341 aagagtagcg ctgttgccga aaccacgctg agtcagatcg attgagctgt catcagaacc
    14401 ttggccaaca tctgcgccgt taccgccgcc gtgctgggta atggtcagat cagagttacg
    14461 ggcgtcagct tgcagagcaa gagcagagtt accgccaccg tactggtaaa tgttcagctc
    14521 tgaatttggg ccgctattat taccgccacc accgtggtta ccgccgccgc cgtactgagg
    14581 aacaacacct gccagagcgc taccagagaa tacgattgct gcaattgctg ctacttttaa
    14641 aagtttcatg taaaaccccc atcggattga tttaaaagtc gaatggaaat taacgttgtg
    14701 tcacgcgaat agccatttgc gactgtctct gcactacaat tgccgttttt tgagtaccat
    14761 actgtgtaat atttgcttta ttaccagaac ctttctggat aatcatcgca gtattaccat
    14821 aagcaccttg cgaaatactg gcatcgttgg cactgcccgc ctgatcaata tatgcaaggt
    14881 tataatctcc tgtctggtca atctttgccc ggttgctact accttcttgc gcaacaaccg
    14941 ccaaaagttt tgagcctccc tgccgtaact gagcactatt attagtccca gcttgaccaa
    15001 ttatggctgc ctgattaaat gaagacttac tcaattcatt taccgcgaag ttatattctg
    15061 aattagctaa atcataacct gctgcggctg caatcccagg cgcacccagt attgttaaca
    15121 tcataaataa caatttgttt ttcatgttgt caccctggac ctggtcgtac atttaagaaa
    15181 ttaaatcatt tcaacttggt tgttaacgca acctgtattt tgttaacgct gcgttacgat
    15241 ggaaagtatg tctgcggaaa tatttttaat aactcacccg cgcgttg
//
LOCUS       DAZUCC010000121        15228 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZUCC010000121 DAZUCC010000000
VERSION     DAZUCC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.121"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..514)
                     /gene="curA"
                     /locus_tag="ACHY09_003150"
     CDS             complement(<1..514)
                     /gene="curA"
                     /locus_tag="ACHY09_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFS3886091.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDIATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGK"
     gene            695..1210
                     /gene="mddA"
                     /locus_tag="ACHY09_003151"
     CDS             695..1210
                     /gene="mddA"
                     /locus_tag="ACHY09_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFS3886092.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERSEPDAI"
     gene            1210..1659
                     /locus_tag="ACHY09_003152"
     CDS             1210..1659
                     /locus_tag="ACHY09_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS3886093.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSNVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(1660..1893)
                     /gene="ydcY"
                     /locus_tag="ACHY09_003153"
     CDS             complement(1660..1893)
                     /gene="ydcY"
                     /locus_tag="ACHY09_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFS3886094.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1979..2152)
                     /gene="ortT"
                     /locus_tag="ACHY09_003154"
     CDS             complement(1979..2152)
                     /gene="ortT"
                     /locus_tag="ACHY09_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFS3886095.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2347..2442
                     /gene="yncL"
                     /locus_tag="ACHY09_003155"
     CDS             2347..2442
                     /gene="yncL"
                     /locus_tag="ACHY09_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFS3886096.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2539..3963)
                     /gene="patD"
                     /locus_tag="ACHY09_003156"
     CDS             complement(2539..3963)
                     /gene="patD"
                     /locus_tag="ACHY09_003156"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFS3886097.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFDEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(3985..4779)
                     /locus_tag="ACHY09_003157"
     CDS             complement(3985..4779)
                     /locus_tag="ACHY09_003157"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3886098.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAVYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(4769..5710)
                     /locus_tag="ACHY09_003158"
     CDS             complement(4769..5710)
                     /locus_tag="ACHY09_003158"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3886099.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWGNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLIMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(5711..6724)
                     /locus_tag="ACHY09_003159"
     CDS             complement(5711..6724)
                     /locus_tag="ACHY09_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3886100.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKSASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVDKKQRYAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDALMADKLCGMTGSFALRPEHIRFNIPGEVQANGT
                     IQAVQYQGAATRFELRLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(6742..7887)
                     /gene="ydcS"
                     /locus_tag="ACHY09_003160"
     CDS             complement(6742..7887)
                     /gene="ydcS"
                     /locus_tag="ACHY09_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFS3886101.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGIRDPYQLTEEQY
                     QAVLKVMRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMSWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(8132..9538)
                     /locus_tag="ACHY09_003161"
     CDS             complement(8132..9538)
                     /locus_tag="ACHY09_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS3886102.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVATQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(9617..10033)
                     /gene="hicB"
                     /locus_tag="ACHY09_003162"
     CDS             complement(9617..10033)
                     /gene="hicB"
                     /locus_tag="ACHY09_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFS3886103.1"
                     /translation="MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(10077..10253)
                     /gene="hicA"
                     /locus_tag="ACHY09_003163"
     CDS             complement(10077..10253)
                     /gene="hicA"
                     /locus_tag="ACHY09_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFS3886104.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIK
                     EPLRKAILKQLGLN"
     gene            10475..10705
                     /locus_tag="ACHY09_003164"
     CDS             10475..10705
                     /locus_tag="ACHY09_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFS3886105.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGHR"
     gene            complement(10797..12758)
                     /gene="rlhA"
                     /locus_tag="ACHY09_003165"
     CDS             complement(10797..12758)
                     /gene="rlhA"
                     /locus_tag="ACHY09_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFS3886106.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(12831..13367)
                     /gene="sutR"
                     /locus_tag="ACHY09_003166"
     CDS             complement(12831..13367)
                     /gene="sutR"
                     /locus_tag="ACHY09_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFS3886107.1"
                     /translation="MENLTRFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQVMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGGEQIVHFHSLIHYPRS"
     gene            13420..14631
                     /gene="ydcO"
                     /locus_tag="ACHY09_003167"
     CDS             13420..14631
                     /gene="ydcO"
                     /locus_tag="ACHY09_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFS3886108.1"
                     /translation="MLRYLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDDQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGVVIVIAQGDVVTTDVVFKPVLPTYISPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            complement(14666..>15228)
                     /locus_tag="ACHY09_003168"
     CDS             complement(14666..>15228)
                     /locus_tag="ACHY09_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFS3886109.1"
                     /translation="NYSDLKETTSATGKPVLRWVDPSFDQSKYDSIVWNPVTYYPVPK
                     PSTQVGQKVLDKILSYTNTEMKEAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQF
                     YEVVPVALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNEST
                     PMAFENIKQVIDDMATDATMFDVNKK"
BASE COUNT         3712 a         4013 c         3721 g         3782 t
ORIGIN      
        1 gtttgccgat ttgacccacc gtcgccccca caggacctgt cgccgcagca acaaccaatg
       61 tttcgccctc tttgggctga ccgatatcca gcagccccat ataagcggta aagcctggca
      121 tccccagcac acccagcgac cacgatggat tttgcggatg atcgccaagt ttcaccagat
      181 catcaccact ggatatgtca tagtcctgcc atccactgta gcccagcacc cagtcgccag
      241 gctggtaatc aggatgattc gactccacaa cacggctcac cgtaccgccg accattacgc
      301 caccaatatc aaccggcggt gaataagatg gctcatcgct catacgtcca cgcatatacg
      361 ggtccagtga caaataaact gtgcgcagta acacctgtcc ttcacccggt gtggcgatat
      421 catcttcttc aagacggaaa ttctccggaa ccggcgcgcc atgcggacgc gaggccagaa
      481 cccaacgtcg attacgctgc ttttgttgcc ccataatgct ctcctgtgct tttggcatga
      541 atccatcaat agtagcaggc tcgcaggaat ggttttgttt gcagccgtca catcccctct
      601 gcaacacgca ataatttcat cgaggaatcc gttgcttcgg gctgttttcg atagtattga
      661 taacgtcatc atcattattt aatcgaggcc aaacatgtcc atccgttttg cccgcaaagc
      721 cgactgtgct gccattgcgg aaatttataa ccacgccgtg ttgtatacgg cggctatctg
      781 gaatgaccaa acggtggatg cagataaccg cattgcctgg tttgaagcgc ggaccatagc
      841 aggttatcca gtgctggtga gcgaagaaga tggcgtagtg acgggatatg cctcgtttgg
      901 cgactggcgt agtttcgatg gttttcgcca taccgtggaa cattcggttt atgtccatcc
      961 cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc cgattgattg atgaagcgcg
     1021 ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg caaaatcagg cgtctttgca
     1081 tctgcaccag tcgctgggat ttgtcgtcac cgcgcaaatg ccgcaggtgg gcactaaatt
     1141 tgggcgctgg ctggacctga catttatgca gttgcaactc gacgaacgca gtgaaccgga
     1201 cgcaatctga tgaatcagtc gctcaccctt gcttttttga ttgccgccgg gattggtctg
     1261 gtagtccaga acacgctgat ggtgcgcatc acccagacct cctctaccat tctcatcgcc
     1321 atgttgctga actctttggt aggaattgtg ctctttgttt ctattttatg gtttaaacaa
     1381 ggtatggcgg ggtttggcga actggtgtcc aacgtgcgct ggtggacact tatacctggc
     1441 ttgctgggat cgttttttgt cttcgccagt atcagcggat atcagaatgt gggagcggcg
     1501 acaactattg cggtactggt cgccagccag ctgattggcg gtctggtgct ggatatcttc
     1561 cgcagccatg gcgtaccact acgggcgttg atcggcccaa tttgtggcgc aattttactt
     1621 gtggttggtg cctggctggt ggccaggcgc acattttaat cagaggatgg tgccaccttt
     1681 ggttaactgt tcgtggcgcg cttccatatc ttctttatgt ttgcgaccgt gatgggcgat
     1741 cgctgtgcga agacgctgct gctgggtata gcgttcttcc cgacttaact ccgcatcatc
     1801 gctcagggcg attaataatt cgttcatatg ggcaatgacg ctctcttcaa tcgcggcatc
     1861 cacgcgcgcg atgacttcat ccagatgcga catactttct ccttagccac ttgtgaaatt
     1921 tatcgattca tccaaatgat ataagtctgc gtggtgccgt caagccagtg agtaagattt
     1981 aaaacagtga aaacaacagc gccaccggga aactcatcgg ccatgttgcc cccaccagaa
     2041 aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag aaaccaggtg ataagaaatg
     2101 cgattgctgc cataaccgca taaaaaacaa gcatgtgttg atagagagac atgtacgggc
     2161 gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat tttttgacgt acatcaagtt
     2221 ttttaaccgg tttgagaaaa aaacatcaaa cagcaacttg cccttatcca tcttcagaat
     2281 gagtattatt gcggattttc ttaacccgta ctatacacat aggggcaatg ataaaaggtg
     2341 gcaaaaatga atgtttccag tagaactgta gtactgataa atgtctttgc tgctgttggt
     2401 ttgtttactc ttatctctat gagatttggc tggtttattt gatgtagtga ttttgcacgc
     2461 agcacgtact gtcagagaaa tcagtgctct gacagtacgc aagatcatct catactctta
     2521 cgatctgcaa tattatcctt aatgtttaac catgacgtgg cggacgacgg tgtaatcctc
     2581 cagcccataa agtgacatat ccttgccgta accagaaagt ttctgcccac cgtgcggcat
     2641 ttcacttacc agcatgaaat gggtattgac ccaggtacaa ccatattgca gccgtgcgct
     2701 gacgcgatgc gccctgccca catctttcgt ccataccgaa gatgcaagtc cgtactggct
     2761 gtcattcgcc cagtttacca cctgttcttc gttgtcgaag agtgtaacac tcactactgg
     2821 accaaatacc tctttttgca cgatggcatc gtcctgtaat gcgccagcca gcagcgtcgg
     2881 cgcatagtaa tagccattac ccttgcgctt ttcaccgcca gtgatcactt tgatgtgccc
     2941 tgtcgctttc gcctcttcta ctgccttgct gacgcgttcg agatgcgcca gcgagcttaa
     3001 aggtccaagc tccgtagact cgtcatccgg cgcaccagat tttaacgttg ccaccgcagc
     3061 acccagtttt tccaccagcg tatcgtaaat gcctttttgc gcgtagatcc gacaagccgc
     3121 agtacaatcc tgtccggcat tgtaatagcc aaatgtacgt acaccttcga ccactgcctc
     3181 aatatccgca tcatcaaaaa caatcactgg cgctttgcca ccaagttcca tatgagtacg
     3241 ctttatggac ggcgcggtat ggctgatgat gtgttcccca gtggcgatag agcccgtcag
     3301 cgacaccatc cgcactttgg gatgaccggt cagcggatcg cccaccgttt tgcctctgcc
     3361 aaacagtaca ttaatcacgc cagccgggaa gatatctttc gccaactctg ccaacttcaa
     3421 cgctgtcagc ggggtaattt ctgatggttt aagcactacg cagttccctg ccgccagcgc
     3481 cggagccagt ttccacgcag ccatcatcag aggataattc cacggtgcga tagaagccac
     3541 gacccccaac ggatcgcgac ggatcatcga agtatgacct tcaagatatt cacctgccgc
     3601 cagaccattc agacagcgcg ccgcaccagc gaaaaagcga aaaacatcga caatcgccgg
     3661 gatttcatca ttgaacgcac tatgcagcgg tttgccacaa ttacgggact ccagttcggc
     3721 aaaaacctga ccattttctt cgataacatc agccagtttc agcagacatt ccgcacgcac
     3781 tttcggcgtg gtttgccccc attcgtcaaa tgctgcatct gccgcgcgaa cagcagcatc
     3841 gacctgctct gcggatgcct cggcaatctc cagtaaaacg tcccccgttg ccggattata
     3901 gacaggctgt ttttcccctt cgccgctaac cagttctccg ttaatcagta acttatgttg
     3961 catagcatat tcctgtatca gtggttattg accattgtcg ccttcgcggg ttagccacca
     4021 ggcccccagg atcggcaagg ttgttaccaa cataaccagc agtgccacca cgttagttac
     4081 cggtacatca cgcggtcgcc caagctgatt gagcaaccac aacggtaacg ttcgttcatg
     4141 acctgccgta aaggtcgtaa cgatgatttc atcgaacgac aaggcaaacg ccagcattcc
     4201 tcctgccagt aacgccgaac tgagattcgg caacactacg tagcggaagg tttgccagcc
     4261 attagcccca agatccattg acgcctcaac cagactccac gaggtgcggc gaaaacgggc
     4321 gatgacattg ttaaagacca ccactacaca aaaagtcgca tgaccgacca cgatggtgaa
     4381 aaatcccggc tccagattga tggttttaaa ggcggttaat aacgccagac cagtgacaat
     4441 gcccggcagc gcaatgggca gcagcagtaa cagcgaaatg gcgtttttgc cgaaaaagtc
     4501 tcgtcgccac agcgcggcag cggccagcgt ccctaacacc agcgcgatta atgtcgccag
     4561 cgccgccact ttaagtgaca gtgtcacggc atcaagaata tcactacgct gtgctgccac
     4621 gctaaaccag cgcagcgtca ggccttgcgg tggaaaacta aacgccgcat cttcagtgtt
     4681 aaacgcataa acggcgataa tcagaatggg aaaatgtaag aaaaccacgc cgccccaggc
     4741 cgccagtttg aggaaaaacg gtgcgcgttc agagtgcatc gaacgctccc agacgtttca
     4801 cgaacgctag gtaaagtgcg atgagtacaa tcggcactag ggtgaatgcc gccgccatcg
     4861 gcatattgcc aatcgccccc tgctgggaat aaaccatatt gccgataaaa tatcctggag
     4921 ggccaaccag ctgcgggaca ataaaatcgc ccagcgtgag tgagaaggta aagatagagc
     4981 cagcggcaat acccgggatt gccagcggca gcaccacata gcgaaaggtt tgtcgtggac
     5041 gtgcgccgag atcagctgac gcctgcaaca atgacggcgg caaacgctca agcgccgcct
     5101 gcaccgggag gatcatgaac ggcaaccaga tatagagaaa caccagaaag cgccccagcc
     5161 cggaagttga cagcgtatta cctcccaccg ccggtagcgt taggaacgca gtcagcagtg
     5221 gttccagccc cagatgttgt aaaaaccact gcgccacgcc atcttttgcc agcaataacg
     5281 tccaggcata ggctttcaca atgtagctcg cccacatcgg caacattacc gcgatataaa
     5341 aaaacgcttt cattttcccg ctggtatagc gcgccatata ccacgccatt ggaaaagcca
     5401 gaatggcgct ggcgatagtg actgccacag ccataatcag cgtgcggaga atgatgtcgt
     5461 aattcgccgg attaaacagc gcacggatat tcgccagcgt cagttccggc gttaccgaca
     5521 tggtgaaatc gtcaaaggta taaaatccct gccataacag tgtcagcagc gagccgaaat
     5581 agacaatgcc aaaccacatt agcgggccaa gcagcagtaa aaacagcccc agccccggat
     5641 taccccagaa gaaaccggac accttaccca gacctggacg tgaaggtgat tgcaatacgt
     5701 tcatcgccat tcacctctcc tcaaccagcg gtaccatcac atcacgcgac caggaaacca
     5761 tcacctgttg tccgggcgtg agcgtggcgg gcagttcttc gcctgtcata ttggcctgac
     5821 tcaccagcag tttttcaccg ccgttcagtc tcagttcaaa acgggtcgcc gcgccctgat
     5881 actgcaccgc ctggatcgtg ccattagcct gcacttcgcc ggggatgttg aagcggatat
     5941 gttccggtcg cagggcgaag cttcccgtca tgccacaaag tttgtctgcc atcagtgcat
     6001 caaaaacatt cgatgtcccg acgaacccgg caacaaacgg cgtgcgcggg cgcatataga
     6061 gatcacgagg ggaatcgacc tgctcaatgc gtccattatt gaaaaccgcc acacgatcgg
     6121 acatcgataa cgcttcgccc tgatcgtggg taacgaagat aaaagtgata ccgagagact
     6181 gttgcagttt tttcagttcc agctgcatct gctcacgcaa tttgagatcc agtgcgccga
     6241 gcggttcatc caacagcaat acgcgcggtt cattcaccaa tgctctggcg atagcaaccc
     6301 gctggcgctg accaccagaa agctgtgacg gcttacgttg ctgaacaaaa ccaagcgcca
     6361 ctttctccag cgcttcttgc gccatagcat atcgctgttt tttatccacg cctttgacca
     6421 tcagcccata ggccacgttg tcaagaatcg acatatgcgg aaaaagtgcg tagtcctgga
     6481 agacggtatt aacatcccgc tcccacggtg gcagattgct ggcggattta ccaaagatag
     6541 agatagcccc gccggaaagc tgttcgaagc cagcaatcag gcgcaggcag gtggttttgc
     6601 cggagccgga cggccccagc atagagaaga actcaccatc ttttatcgca atactgacgc
     6661 catctactgc gcgcacgtca ccgtacaacc gtgagacgtt gtcaaactcc actgcgtacg
     6721 tcataaaaca ccccagcgaa gttagcgacc gcccataatg gcaatgtaat cctgcgtcca
     6781 gcgactgtag ggaacaaact tgcccccttc tgctataggc gttttccaga aggcgatttt
     6841 atcgaaatag ttaaaaccgt tggtttcaca acctttttca cctaataacg gactggcttt
     6901 acacccttgc ggcactactg gtaacgagcc aaaccaggcc gccacatcac cctgcacttt
     6961 tggcgttaat gaccagctca tccatttgta ggcgcaaacc ggatgtttcg cttcgctatg
     7021 tagcatggtg gtgtcagccc aaccggtaac gccctccttc gggaaaacgg tagcgacagg
     7081 ctggccttcg gctttcaggg cgttggcctg atagggccat gcactggaag caaccacacc
     7141 ctcgtttttg aaatcgctca tttgcacggt agtgtcatgc cagtagcgat ggatcaaact
     7201 atgttgatcg cgcatcactt tcagtaccgc ctgatactgt tcttcggtga gttgatacgg
     7261 atcgcggatg cccaactgcg gctgagtggc tttaacgaac aacgcagcgt ccgcaatgta
     7321 gatagggcca tcataagcct gaacgcggcc tttattgctc ttgccgtccg gcagattctg
     7381 ctcaacaaaa accacttgcc agctatccgg cggcgtcggg aaggttttag tgttgtacat
     7441 cagcaggttc ggcccccatt ggtaaggtgt gccgtaaact ttgccgccaa cgttaaacca
     7501 gtcgccttta accacgcgcg gatcgagcgc tttccagttg ggaatcaagg cggtattaat
     7561 tggctgcacg cgtttaccca taatcaaacg cagcgaggca tcgccggatg ccgtaaccag
     7621 atcgtaaccc cctttggcca tcagactgac catttcatcg gaagtcgcgg cggttttaac
     7681 attcaccgcg cagcctgtct ctttttcgaa ttgcgttacc cagtcgtatt gtttatcagt
     7741 ttgtccgcgt tcgatgtatc ccggccaggc gataatatcc agtcgccctt ccggtttatc
     7801 taaattggta ggtggttcgg cggcgtgagc ggtcattatt gtcatggtga gcgcacacag
     7861 gctgctgcgg gcaaatgtct tgctcataaa gtcttactcc tgtcgtaatt aattgttcag
     7921 ccgccgtgcc gtaaaaatat ctccttgttt aaccatagac ggcggctagc aggcacacct
     7981 tcgcccctga gaaaatttaa tcaggttgtg agctaccgcg cattatgccg tatgtaaggt
     8041 tatcttctgg agtatagaca aggagttagg aaatatgact gctattcgaa attcatatta
     8101 cccaacctga gaggaattta attaaacgct attacagccg ttcttgaata agtttgccta
     8161 attgtttaac cgcctgttct tcacgttctc cccactgcca cgcggtatta aaacggaaaa
     8221 aacgtgacca gttttcaccg gtagaaaaca ttttacccgg cgcaatactg atatgatgcg
     8281 tcagtgctgc caggcttaat tcgcccgcat ctaacggctc ggggagctcc agccagagaa
     8341 aataaccgct gtcattatga tgaattttca cttccgcagg cagataacgc agcagtgctt
     8401 gccaggcacg ttgtttacgt tccgcaagct ggcgacgcag gcgacgaaga tgggcgtcgt
     8461 atcggcgcgt ggaaaggtaa tccaccagcg caagctgcat cggtgagctg gtggaaagcg
     8521 tactcatcaa ctgcaagcgt tgaattttac gtgcatgttt tccggcggcg acccaaccaa
     8581 tacgaaaacc aggcaccaga catttcgaaa acgaagagca atgcaaaaca ccatcgtggc
     8641 gatcccacgc tttcgcaggc agcggttttt cccgtccaaa ataaagttcg ctgtaaacgt
     8701 catcttcaat cagcgttacg ttgtactgat tgagcaacgc taccagccgc gcttttttct
     8761 gcggcgttaa ggtaaatccg agtggattct ggctattagt catcagccag cacgctttca
     8821 ccggatactc ctgcaacgcc agttccagcg ccttgagatc aatcccttct ttaacgtccg
     8881 tcgccaccga taacgccttc agccgtaacc gctccagcgc ctgcaacgca ccgtagaaac
     8941 aaggattctc tactatcacc caatcgcccg gttcagtgac cgcttgtaaa ctgaggttta
     9001 atgcctctaa cgccccggca gtaatgacaa tttcatcggg tgaaatggtg atgccctgta
     9061 aggcatagcg acgagctata gcctgacgca gttcagcgtt tcctggcggt aagttttcaa
     9121 tcacgctcat cgccgtggcg gttttgctta cctgtgccag cgagcggttg agttgttgga
     9181 gggggaaaag tcgcgggtcg ggaaaggccg aggcaaacgg aactaccgac ggatcgcgac
     9241 tggcctgcaa catatcaaaa atataggtgt tgatatcgac tgcttcatct cgagtgactg
     9301 gaatgactgg cgctttcggc atttttattg cctgtgtcgc aacgtaataa cccgactgcg
     9361 gtcgcgcgat aatatgtccc tgactttcga gcaactgata ggcatggctg acagtcataa
     9421 agctcatgcc agaaagcgcc acctggtcac gcaacgaagg caaacgatcg ccaggttgcc
     9481 agatacccga cgcaatctgc tcgcgtaatt gttctgcaag ctgctggtat tttttcatta
     9541 tcgaatcgta attatgtgca gatgattcgg cagtctatat cagttgttaa aaatgacaac
     9601 ttttcattaa ctgttatcaa accatcacca gtgataactc tttgccaagc gccttagccg
     9661 caagctggac ggcgtcgatt tttgtagcat ggtgcaagtt aaataagcga gtaatctcct
     9721 gtttaggttt gccaattcgt ctggctaact cttgctgagt gatttctgac tgtaaaaaag
     9781 catttaacag caatacttta gaggcgatac ttaaaggcac ttcaataaag tgatcgttgt
     9841 tagttaatgg cgaaggcaac gggattagct cattatcttc aaaataaaaa tcaaatgcgg
     9901 tgagtaaggc atcttttgct gcttccatcg cttcagcaac agtttctccc tgcgtcaacg
     9961 cttcagggat atctacaaaa gaaaccacat aaccgccttc cggcgcagga gtaagagtga
    10021 cgggataacg cataattttg actgtctctc gcatgtcatt aaccttctta tggcgattaa
    10081 tttaaaccga gttgcttcag gattgcttta cgcaatggtt cttttatctc atcactgggg
    10141 tgacgcggca tgacactgcg tcgcccttga aacctgagtt tcaaatggtt gctgccattc
    10201 gccacatcga cgccctgaga ttcgagccaa cgtctgaact cgctttgttt cacatcgcct
    10261 ccctgccgct gagtatatca acaaagtaaa cattagtgtt tacctccgtg tgtaatttgc
    10321 tcgaaaatag cactaaatcg ccgcaacggc agaatgcaat gcaaaatcag gaaggaagat
    10381 tccagaattt ctttgcgcta atttgcaaaa ctgaataata acctcaagaa acgatcggca
    10441 tcatagcgac aaaaggtaag aaaaaggtgg caggatgttt acgaaggcgt tatcggttgt
    10501 cttattaacg tgtgcactgt tctcaggaca actcatggca gggcacaaag gacatgaatt
    10561 tatgtgggta aagaatgtgg atcatcagct gcgtcatgaa gcggacagcg atgaattgcg
    10621 tgctgtggcg gaagagtcgg cggaaggttt gcgcgagcat ttttactggc aaaaatcgcg
    10681 caaaccagaa gcgggacatc gttgagtcat ttgttgccgg atgcggcatg acgccttacc
    10741 cggcaggcaa tatcatgcag atttgatgag ttgtgagtaa ccagaaaact ggcgttttac
    10801 ccttttcgct tcggtaaagt tttcagcagc tcatccggac ttacggatgc cactacgctt
    10861 cccggtaacg gcattttcag tatgtgattt ttgattttgc caatgacgtg catctcgcat
    10921 gggcggcaat caaactttag cgttaatact tcatcgccgt taaccagttg cattggcgtc
    10981 gccttccagc ttttgatatt gcctttcgcc tgtttcgggc acagattgaa ggcaaagcgc
    11041 agacaatgct tagtgatcat caccgggact tcgcccttct cttcatgcgc ttcatacgcc
    11101 gcatcaatca gttgcacacc atagcgatga taaaattcac gcgctttctg gttgtaaacg
    11161 ttcgcgagga aactcaaatg cgtttgcgga taaaccggcg caggatcagc aaccggttta
    11221 cggctgccgc gctggtaact ggcaagacgc gcagcatcta gcatgtcggc ggcttcacgg
    11281 cggaactggt ttaacagact gtttggtaca aacagcgcac ccggcaaatt aatttgcaca
    11341 tcgcgggcgt aatagatggt ttgccccagt tttgccagac catcctttag attgttcatt
    11401 gctttttcgg cgttattggc ttcgtcgaac tgcccgtcca gcgtatgcgt gatgctgaca
    11461 ccctcttcac tggtgagggt cagaatcagt tgttcctgcc agccgcccag ttcaatgtct
    11521 accgccaccc gacgttcgct ggaggttttt gtcagtgcct gctgccagtt atgatcaaga
    11581 ttacggttta gtgggtgatg cgggcgaatt ttgtgcaact ctgctggcat ttcattgggc
    11641 cagacgcggt actgattttc tccggttttc tcgaccgtat tggcacgaaa gccgacgact
    11701 tcacgtttaa tcatcacgtt caggccatcg ccatttgcca gtggctcggt aacggcaaca
    11761 tcgagatgat ctttcgccac tttcaatact tcacccaccg gcaggccgat aaatttcggc
    11821 gaatcgaacg cgccaatatc gcctttacgg gcattcacaa aataatctgt gctaccacgg
    11881 tggaaagtct tttccgtcga tggaacaaag aaatgttcag tacgacctga tgaagcacgc
    11941 gtcagatcgc cacgttcttc aataatggca tccagcatct ggcgataatg ggcggtgata
    12001 ttcttcacgt agctcatatc tttgtaacgc ccttcaatct tgaaggagcg tacgccagca
    12061 tcaatcagcg cgccgaggtt ggcagtctga tcgttatctt tcatcgacag cagatgtttt
    12121 tcataggaaa ccacccgccc ctgatcgtct ttcaatgtgt atggcaaacg gcacgcctgc
    12181 gagcaatcgc cacggttggc gctacgccct gtttgcgcat gagaaatgta gcactgaccc
    12241 gaataggcca cgcacagtgc gccatgaata aagaattcaa tggtcgcgtc cgtggcctgg
    12301 tgaatcgcgc ggatctgctc aagattcagc tctcgcgcca gcacaatctg cgtgaagcca
    12361 acatcagaga ggaacttcgc tttttctact gtacgaatgt cgcactgcgt actggcgtgc
    12421 agttcaatcg gcggaatatc aagttccaga atccccatat cctgaacaat cagcgcgtcg
    12481 acgccggtct ggtagaggtc ggtaatcagc cgttgcgcag gttccagctc atcatcatgc
    12541 aaaatggtgt taagcgtgac gaaaattttt gcaccataac gatgggcaaa tggcaccagt
    12601 tcggcaatat ctttcaggct attactggca ttatgacggg caccaaaacc agggccgccg
    12661 atataaacag catcggcacc gtgcaaaata gcttcgcggg caatggcggc atcgcgtgcc
    12721 gggcttaaca gttcaagtcg atgagaagat acggtcacat tcttatccag tagctgaaaa
    12781 atggcggcta ttttagccac aagcgcgacg ttgcgaaata gttttcccgc ttaactgcgt
    12841 gggtaatgga tgagggaatg aaaatgtacg atttgctccc caccattacg atagacatgc
    12901 gtaacgtctg cagcaaatcg agcgccttct ccacaattaa ggctctgcca ttcaccatca
    12961 acgcacagat caagttgccc atcaatgacg acaacatgtt cgattactcc cttctcatgg
    13021 ggcgtcgatt cacttattgc accaggtgcc atctgaatcg agaagtgttc aaagcagagt
    13081 tgtggatcat aaggaaacag cgaagtgatc accatcacct gctgttgcgg atcataaact
    13141 gaaggcgtag cggactgcgg cggagaaata aatgtcgaaa atggcacatt caacccggtg
    13201 gcaattttcc ataatgtcgc gaccgtcggg ctggactcat tacgctcaat ctgccctaac
    13261 atcgctttag aaacgcccgt cgcttctgcc agtcgcgaaa gactccagcc gcgctgctga
    13321 cgtagttgtt taagtgtggt ggataaaaag cgagtgagat tttccatgcg actcctccgg
    13381 caaaacggaa gtttatcact tgtgcgttat aacggacaaa tgctacggta cctgtatgct
    13441 ataacgcacg aggtgaccat gcgtctgttt tctattcctc cacccacgct actggcgggg
    13501 tttctggcgg tattaattgg ctacgccagt tcagcggcaa taatctggca agcagcgatt
    13561 gtcgccggag ccaccactgc acaaatctct ggctggatga cggcgctggg gctggcaatg
    13621 ggcgtcagta cgctggctct gacattatgg tatcgcgtac ctgttctcac cgcatggtca
    13681 acgcctggcg cggctttgtt ggtcaccgga ttacagggac taacacttaa cgaagccatc
    13741 ggcgttttta ttgtcaccaa cgcgttaata gtcctctgcg gcataacggg actctttgct
    13801 cgtctgatgc gcattattcc gcactcgctt gcggcggcaa tgcttgccgg gattttatta
    13861 cgctttggtt tacaggcgtt tgccagtctg gatgatcaat ttacgttgtg tggcagtatg
    13921 ttgctggtat ggctggcaac caaggccgtt gcaccgcgct atgcggtaat tgccgcgatg
    13981 attattgggg tcgtgatcgt tatcgcgcaa ggtgacgttg tcacaactga tgttgtcttt
    14041 aaacccgttc tccccactta tattagccct gatttttcgt ttgctcacag cctgagcgtt
    14101 gcactccccc tttttctggt gacgatggca tcgcaaaacg caccgggtat cgcagcaatg
    14161 aaagcagctg gatattcggc tcctgtttcg ccattaattg tgtttactgg attgctggca
    14221 ctgttttttt cccctttcgg cgtttattcc gtcggtattg cggcaatcac cgcggctatt
    14281 tgccaaagcc cggaagcgca tccggataaa gatcaacgtt ggctggccgc tgccgttgcc
    14341 ggcatttttt atttgatcgc aggtctgttt ggtagtgcca ttaccgggat gatggctgcc
    14401 ctgcccgtaa gttggatcca gatgctggca ggtcttgcgc tgttgagtac cattggcggc
    14461 agtttgtatc aggcgctgca taatgagcgt gagcgcgacg cggcggtggt ggcatttctg
    14521 gtaacggcaa gtggattgac gctggtcggg attggttctg cgttctgggg attaattgcc
    14581 ggaggcgttt gttacgtggt gttgaattta atcgctaaca gaaaccgata ataaaaagcg
    14641 ccatctaacg atggcgcaac tggtattact ttttgttaac gtcaaacatg gtggcatcgg
    14701 tcgccatgtc atcaataact tgtttaatat tttcgaaagc cattggcgta ctttcgttat
    14761 tcaggtcttt accttcgccc tgacgaacga ctttgataac cggtttatta gtcgctgcat
    14821 caatcagctc accttcaaaa tacaggcgag tatccatggt acggtggccc gtcgccattt
    14881 gcgtccccgc aaccactaat gcaacaggaa ccacttcata gaattgcagc ccttctttgc
    14941 tggtatctac accggtaatg gccccacgga aaatcagact acgcggccca gcggtggtaa
    15001 ccagtggttt acgctgcgca atcgcttctt tcatttcggt gttggtataa ctcaaaattt
    15061 tatccagaac tttctgccct acctgggtag atggtttcgg taccggataa taagtgactg
    15121 ggttccagac gatgctgtca tatttgcttt gatcaaaact cgggtctacc caacgtaaaa
    15181 caggtttacc tgttgctgag gttgtttctt ttaaatcaga gtaattgt
//
LOCUS       DAZUCC010000122        15112 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZUCC010000122 DAZUCC010000000
VERSION     DAZUCC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.123"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..1291
                     /gene="uraA"
                     /locus_tag="ACHY09_003169"
     CDS             2..1291
                     /gene="uraA"
                     /locus_tag="ACHY09_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFS3886110.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            1386..2087
                     /gene="hda"
                     /locus_tag="ACHY09_003170"
     CDS             1386..2087
                     /gene="hda"
                     /locus_tag="ACHY09_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFS3886111.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(2193..2552)
                     /gene="arsC"
                     /locus_tag="ACHY09_003171"
     CDS             complement(2193..2552)
                     /gene="arsC"
                     /locus_tag="ACHY09_003171"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFS3886112.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2573..4036)
                     /gene="bepA"
                     /locus_tag="ACHY09_003172"
     CDS             complement(2573..4036)
                     /gene="bepA"
                     /locus_tag="ACHY09_003172"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFS3886113.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4249..5310
                     /gene="yfgO"
                     /locus_tag="ACHY09_003173"
     CDS             4249..5310
                     /gene="yfgO"
                     /locus_tag="ACHY09_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS3886114.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(5409..5879)
                     /gene="bcp"
                     /locus_tag="ACHY09_003174"
     CDS             complement(5409..5879)
                     /gene="bcp"
                     /locus_tag="ACHY09_003174"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFS3886115.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(5879..6451)
                     /gene="gcvR"
                     /locus_tag="ACHY09_003175"
     CDS             complement(5879..6451)
                     /gene="gcvR"
                     /locus_tag="ACHY09_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFS3886116.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            6597..7475
                     /gene="dapA"
                     /locus_tag="ACHY09_003176"
     CDS             6597..7475
                     /gene="dapA"
                     /locus_tag="ACHY09_003176"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS3886117.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASMKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            7492..8526
                     /gene="bamC"
                     /locus_tag="ACHY09_003177"
     CDS             7492..8526
                     /gene="bamC"
                     /locus_tag="ACHY09_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFS3886118.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            8739..9452
                     /gene="purC"
                     /locus_tag="ACHY09_003178"
     CDS             8739..9452
                     /gene="purC"
                     /locus_tag="ACHY09_003178"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS3886119.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            9620..10483
                     /gene="ypfJ"
                     /locus_tag="ACHY09_003179"
     CDS             9620..10483
                     /gene="ypfJ"
                     /locus_tag="ACHY09_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFS3886120.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            10498..12513
                     /gene="tmcA"
                     /locus_tag="ACHY09_003180"
     CDS             10498..12513
                     /gene="tmcA"
                     /locus_tag="ACHY09_003180"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFS3886121.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATYVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPQTPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENAIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDIVLSADDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERLRDRITQWQFFH"
     gene            12587..13285
                     /gene="ypfH"
                     /locus_tag="ACHY09_003181"
     CDS             12587..13285
                     /gene="ypfH"
                     /locus_tag="ACHY09_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS3886122.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     TATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(13366..13566)
                     /gene="ypfN"
                     /locus_tag="ACHY09_003182"
     CDS             complement(13366..13566)
                     /gene="ypfN"
                     /locus_tag="ACHY09_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFS3886123.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVNHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(13594..14721)
                     /gene="dapE"
                     /locus_tag="ACHY09_003183"
     CDS             complement(13594..14721)
                     /gene="dapE"
                     /locus_tag="ACHY09_003183"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFS3886124.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(14725..15081)
                     /gene="yffB"
                     /locus_tag="ACHY09_003184"
     CDS             complement(14725..15081)
                     /gene="yffB"
                     /locus_tag="ACHY09_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFS3886125.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         3385 a         3796 c         4138 g         3793 t
ORIGIN      
        1 tatgacgcgc cgtgctatcg gggtgagtga aagaccgcca cttttacaga caatcccgct
       61 tagtttgcaa catttgttcg ccatgtttgg tgcaaccgtc ctggtgcccg tcttatttca
      121 tattaacccg gcgactgtac tgttatttaa cggtatagga acactgctgt atctcttcat
      181 ctgtaaaggg aaaattccgg cttatcttgg ttccagcttt gcctttattt caccggtatt
      241 gttactgttg ccgttggggt atgaagttgc gctgggcggc tttattatgt gcggcgtgct
      301 gttctgcctg gtttctttta tcgtgaagaa agcggggacc ggctggctgg acgtgctgtt
      361 tccacctgcg gcaatgggcg caatcgttgc cgtcatcggt ctggagctgg cgggcgtagc
      421 tgccggtatg gcgggtttac tcccggctga agggcaaacg ccagactcca aaaccatcat
      481 catctctatt accaccctgg cggtcacggt tttaggttcc gtgctgtttc gtggtttcct
      541 ggcaattatc ccgattttaa ttggcgtgct ggtggggtac gcgctctctt tcgcaatggg
      601 aattgtcgat accacgccga ttattaatgc tcactggttt gcgctgccga ccctctatac
      661 gccgcgcttc gagtggtttg ccattctgac tattctacca gcggcgttag tggttattgc
      721 cgaacacgta gggcacctgg tcgtaacggc taatatcgtc aaaaaagatc tgctgcgcga
      781 tccaggtctg caccgttcga tgtttgctaa cggcttgtcg accgtgatct ccggcttctt
      841 tggctccacg ccaaacacca cttacggaga aaacattggc gtgatggcga tcacccgtgt
      901 ttacagtacc tgggttatcg gcggtgcggc gattttcgct atcctgcttt cctgcgtcgg
      961 taaactggct gcagctatcc agatgattcc attgccggtg atgggcggcg tttcgctgct
     1021 gctttacggt gtcatcggtg cttccggtat tcgtgtgttg atcgaatcga aagtggatta
     1081 caacaaagca cagaacctga tcctgacttc cgttattttg atcatcggcg tcagtggcgc
     1141 gaaggtaaac attggcgcgg cggagctgaa aggtatggcg ctggcgacca tcgtcggtat
     1201 cggcttaagt ttgatcttca aactgattag tatgctgcgc cctgaagaag tggtgctgga
     1261 cgcagaagac gcggatataa cagacaaata atgtaccgac cgggcagtgt tgctgcccgg
     1321 ttcaaacatc atgggattct gtggtaaact tctcgcgatt ttgtgaaatc ctggttgagg
     1381 tatctctgaa cacaccggca cagctctctt tgccacttta tcttcctgac gacgaaacct
     1441 ttgcaagttt ctggccgggg gataactcct ctttactggc cgcgctgcaa aacgtgctgc
     1501 gtcaggaaca tagcggttac atctatctct gggcacgcga aggcgcgggg cgcagccatc
     1561 tgctgcacgc ggcttgcgcg gaattgtcgc agcgtggcga tgcggtgggc tatgtcccgc
     1621 tggataaacg cacctggttt gttccggaag tgctcgacgg tatggagcat ttgtcgctgg
     1681 tctgtatcga caacattgag tgtattgcag gcgatgagtt gtgggagatg gcgattttcg
     1741 atctctacaa tcgaattctg gaatcgggca aaacacggtt gttaatcacc ggcgatcgtc
     1801 caccgcggca gttgaatctt ggattaccgg atctcgcgtc gcgtctcgac tgggggcaga
     1861 tctacaaatt gcagccactt tctgatgaag ataagttgca ggcgctacag ttacgcgcgc
     1921 gtttgcgtgg ttttgaactg ccggaagatg tggggcgttt cttgctgaag cggctcgaca
     1981 gagagatgcg cacgttattt atgacgttgg atcagttgga tcgtgcgtcg attaccgcgc
     2041 aacgtaagct gaccattccg tttgtgaaag aaattctgaa actgtagaca agacgtttat
     2101 tgtcggatat gcacccatag tattcgtcgg ccggataaga cgcggcaagc gtcgcatccg
     2161 gcaatgtgct tcaactagcg atggagcact tcttaaccca cgatttccag tacctgctcc
     2221 ggtggacgac caatccgcgc tttgccattc gcgaccacaa tcgggcgctc cattagcttc
     2281 ggattatcca ccattgcctg aatcagcgcc tcttcgctaa gcgaactgtc agccaggttc
     2341 agttctttat aaagatcctc tttctggcgc atcaattcac gagcgctatt catccccagc
     2401 attttcagca aatcacgcag cgttgccgca tcggcgggtg tctcaaggta gagcaccact
     2461 tctggctcca cgccgttttc tttcagcagg ttcagcgttt cccggctctt tgaacagcgt
     2521 gggttatggt agatttttac ctgtttggtc attgttcttc ctttaatgcg aattacatct
     2581 tggtataagg cttaaagcgt tcctgcagct ggcgcaactg gtcgatgcgc gcatcgtaac
     2641 gcgcttgttg caggctgcct aatttcacct gcgaactggc gctactcaac agcgaaatgg
     2701 cctgatcgag tcgtccggcg agcgcataac cttctgctcg cgcagccagc tcctgatcgc
     2761 ggttatttag cgcggcttcc gcctgtgcca gcaaatccca gccgttgctg tcatctttat
     2821 tattaaaggt gtagcgattc agaatattgg ccgcttcttg tggttgaccg ccttgcagat
     2881 aagcgttcgc caggttgagc tgcaacaccg gattggtgcg taaatcgcgg gcatttttca
     2941 gacgattgat cgcctcattg gctttgtttt gcccaagatc gatatcagta gccagatcga
     3001 gataccatgc gttgccaggt tctgccgcca gtaacggttg cagcgtttta cgcgcctcgt
     3061 cgtatttatt ggcttccatc gcctgtaaag cacgaccata ttgcgccgct cgttgctgac
     3121 gaacgtttcc tttcgcccat tcatccagca aatcactggt gagctggtta cgtccggaat
     3181 tatacatccc cagtgtgcgc gctttcgcca gatagaaatc ttccgacgac tgcaccacca
     3241 tcgggcgcat ctgattagca cggttgcggg catctgccag acgactttcc ggcaacgggt
     3301 gagtcaataa aatttccggc ggacgcgagg agtaacgcgc ctgatcgagt aatttttcga
     3361 ggaaggtcgg catcgcctgc ggatcgaatc ctgagcgttg cagcacctga ataccaatgc
     3421 ggtccgcttc ctgttcattt tgctgggtga agctgatcat cccctggcgc gttcccgcca
     3481 gtgtaccggt cagcgcagcc atccccgcct gcggactggc catcgccagt aaaatagaac
     3541 ctaacgcgcc gacccaggtc agcggcgcgc tgcgctgctg atcttccatc gctcgcgcca
     3601 ggtgacgttg ggtgacgtgg gagatttcgt gcgccataac tgaagccagt tgactttcgt
     3661 tatcggaata acggaacagg gcagagtgca gcaccacgtt gccgccaaag aaagcaaagg
     3721 cgttaatttc atcgttgttg atcagaaaga aatgaaacgg tgtcttaacc gaattggcat
     3781 gcgaaaccag acgcattcct agcgaattaa tgtattgcgt taacagcgga tcattaatta
     3841 acggcgcgct gccgcgtagc tggcggacat aatagtcgcc catctgcatt tcctgaccaa
     3901 tggaaagtgt gcttcctgcg gaggttccca tatccggcaa ggtgtctgcg ctgtcggcaa
     3961 acgccggggc tacctgacca atggtcatag cagcaatgag ggttgcaacc aggttttttt
     4021 tcaactgcct gaacataacc tctatcctgt atttctggag atagtcattt gaccgatact
     4081 gtgaaatatc gttcccaccg cgccgccttt gagtgtaacg gcgaagatta gcccactcaa
     4141 atgacttttc agtttcctga atccgtcata aaaagcgaag tcttttttgt ggcatttcga
     4201 tacaattgcg gatcgcaatc ccgctcttga ggttcaggga aggtctgtat gctcgaaatg
     4261 ttgatgcaat ggtatcgccg ccgttttagc gacccggaag cgattgcctt gctggttatt
     4321 ttagttgccg gatttggcat tatctttttc tttagtggcc tgcttgctcc gctgttggtg
     4381 gctattgtgc tggcctattt gctggaatgg ccaaccgtgc gcctgcaatc tattggctgc
     4441 tcccgccgct gggcgacgtc gattgtattg gtggttttcg tcggtatatt gctactgatg
     4501 gcgttcgtgg tactgcctat cgcctggcag cagggcatct acttaatccg cgatatgccg
     4561 gggatgctca ataagctttc tgactttgcc gccacgttgc cgcgccgcta tccggcgtta
     4621 atggatgcgg gcattattga tgcaatggcc gaaaatatgc gcagtcggat gctgaccatg
     4681 ggcgattcgg tggtgaaaat ttccctcgcc tcgctggtcg gtttgctgac cattgccgtc
     4741 tatctggtgc tggtaccatt gatggtcttc ttcctgctga aagacaaaga gcagatgctg
     4801 aacgccgttc gccgggtgct gccgcgtaac cgtggactgg caggacaggt gtggaaggag
     4861 atgaatcaac aaatcaccaa ctatatccgc ggcaaagtgc tggagatgat cgtggtgggg
     4921 attgccacct ggctgggatt cttactcttc gggctgaact attcgctgct gctggcggtg
     4981 ctggtcggct tctcggtact tattccgtac attggtgcat ttgtggtgac cattccggtg
     5041 gttggcgtgg cgctattcca gtttggtgct ggcacggaat tctggagctg cttcgcggtg
     5101 tatctgatca ttcaggcgct ggacggcaac ctgttagtac ctgtgttgtt ctccgaagcg
     5161 gttaacctgc atccgctggt cattatttta tcagtagtga tcttcggtgg tttgtgggga
     5221 ttctggggcg tattcttcgc cattccattg gcgacgctga tcaaagccgt gattcacgcc
     5281 tggcccgatg ggcaaatcgc gcaagactga ccttcagcga aaaaatttga aacgagtgtt
     5341 gcagggcagt gagaagatgt gagatgtgct ggccgcaagc gcagccagca cggaatggag
     5401 caaagtaatc aggcgtgttc tttcagccag ttcagcacaa cgtcgtggtg attgctggtt
     5461 ttgaaatcgt caaagacatg ttcgattttg ccatcagcgt caatcaggaa gctgatgcga
     5521 tgaataccat cgtaggtttt gcccatgaag gacttttcac cccagacgcc gaattgttcg
     5581 cacacctggt ggtcctcatc agacaggagc gtaaagttaa gcagctcttt ttccgcaaaa
     5641 cgggagagtt tttcgggttt atcggtgctg atacccagca catcaacgcc cgcttttttc
     5701 aactcatcca tgttatcgcg taagccgcag gcctgtacgg tacagccggg ggtcatggct
     5761 ttcgggtaga aataaaccag aacacgctgt ccctggaagt cggtcaaatt aacttgttct
     5821 ccgtcttgat ccggcaagct aaatttcggt gcgatatcac cggctttcag tggattcatt
     5881 acttaactcc atcctgttca tcatgttggg aataattgac gacgttaata ctgccttgtg
     5941 cattgagttc tgtacatagg gctttgaatg cttgctcaat atttgctgcg tccgcagatg
     6001 cggggctgtg ggcggttatc tgaatatgca actgcgcagc ccgttcattt tcagcaggtt
     6061 gcgtgcgcga caccagctcc gcaatgttca tatgatgcgc gtcgaaaagt gctgtgaagc
     6121 gttcaattaa atgcggggag tctgccacat cgacctgaac ccagacagat gctggcattg
     6181 gcggacgcgg acgcgccgtc gtgcgcttca tcacgattaa aagatccagc tcggcacctt
     6241 tcaacggtaa cgttgattca atcagagtaa tggcattcca tgaaccggaa agcagcatga
     6301 taaacgtgaa ctcttctccc agcatcgcca ggcgactgtc ttcaatatta cagccgcaac
     6361 tactgacatg acgggtgatg gtgttcacaa ttccagggcg atcggcaccc aacgcagtga
     6421 tcaccagata atgttgcgat gacagtgtca aactggttat tccttcaagg ggtgagttgt
     6481 tcttaaggaa agcataaaaa aaacatgcat acaacaatca gaacggttct gtctgcttgc
     6541 ttttaatgcc ataccaaacg taccattgag acacttgttt gcacagagga tggcccatgt
     6601 tcacgggaag tattgtcgcg attgttactc cgatggatga aaaaggtaat gtctgtcggg
     6661 ctagcatgaa aaaactgatt gattatcatg tcgccagcgg tacttcggcg atcgtttctg
     6721 ttggcaccac tggcgagtcc gctaccttaa atcatgacga acatgctgat gtggtgatga
     6781 tgacgctgga gctggctgac gggcgcattc cggtgattgc cgggaccggt gctaacgcta
     6841 ctgcggaagc cattagcctg acgcagcgct tcaatgacag cggtatcgtc ggctgcctga
     6901 cggtaacccc ttactacaat cgtccgtcgc aagaaggttt gtatcagcat ttcaaagcca
     6961 tcgctgaaca tactgacctg ccgcaaattc tgtataatgt gccgtcccgt actggctgcg
     7021 atctgctccc ggaaacggtg ggccgtctgg cgaaagtaaa aaatattatc ggaatcaaag
     7081 aggcaacagg gaacttaacg cgtgtaaacc agatcaaaga gctggtttca gatgattttg
     7141 ttctgctgag cggcgatgat gcgagcgcgc tggacttcat gcaattaggc ggtcatgggg
     7201 ttatttccgt tacggctaac gtcgcagcgc gtgatatggc ccagatgtgc aaactggcag
     7261 cagaagggca ttttgccgag gcacgcgtta ttaatcagcg tctgatgcca ttacacaaca
     7321 aactatttgt cgaacccaat ccaatcccgg tgaaatgggc atgtaaggaa ctgggtcttg
     7381 tggcgaccga tacgctgcgc ctgccaatga caccaatcac cgacagtggt cgtgagacgg
     7441 tcagagcggc gcttaagcat gccggtttgc tgtaaagttt agggagattt gatggcttac
     7501 tctgttcaaa agtcgcgcct ggcaaaggtt gcgggtgttt cgcttgtttt attactcgct
     7561 gcctgtagtt ctgactcacg ctataagcgt caggtcagtg gtgatgaagc ctacctggaa
     7621 gcggcaccgc ttgcggagct tcatgccccg gctggaatga ttttgccggt gacctccggc
     7681 gattatgcaa tcccggtgac caacggtagt ggtgctgtcg gtaaggcgct ggacattcgt
     7741 ccaccagccc agccgttggc actggtttct ggcgcgcgta cccagttcac gggcgatacc
     7801 gcttcattgc tggtggaaaa tggtcgtggc aatactctgt ggccgcaggt ggttagcgtg
     7861 ctgcaggcga aaaactacac catcacccaa cgtgatgatg ctggtcagac actgaccacc
     7921 gattgggtac aatggaaccg tctggacgaa gacgagcagt atcgtggtcg ttatcaaatc
     7981 tctgttaagc cgcagggtta tcagcaggcg gttacggtta aactgctgaa cctggaacag
     8041 gcgggcaaac cggttgcaga cgcggcttcc atgcagcgtt acagcacgga gatgatgaac
     8101 gttatttccg ccggtctgga taaatctgcc actgacgccg cgaacgctgc gcaaaatcgt
     8161 gcctccacca ctatggacgt acaaagtgca gctgatgaca ccggtttacc aatgctggtc
     8221 gtacgcgggc cgttcaatgt ggtttggcaa cgtctgccag ctgcgctgga aaaagtgggc
     8281 atgaaagtga ccgacagcac ccgttcgcag ggcaacatgg ccgtaactta caagccgctg
     8341 tctgacagcg aatggcacga actgggcgcg agcgatccag gtctggcatc cggtgactat
     8401 aaactgcagg ttggcgattt agataaccgc agcagcctac agttcatcga tccgaaaggt
     8461 catactctga ctcagagtca gaacgacgcg ctggtagctg tcttccaggc tgcgtttagc
     8521 aagtaaaaat acagggctgg agtcatccgg cccttttttc tgatatgata cgcaaacgtg
     8581 tgcgtcggca gaaaaacgcg attttagcgg taattcgcac gaaatttgtt tgtcggacgt
     8641 aggtcggata aggcgttcac gccgcatccg acaaaacatc cggcacacca gacagcaata
     8701 gattttaaaa cgttaattca cacccaggag tgataaagat gcaaaagcaa gctgagttgt
     8761 atcgtggtaa agcgaaaacc gtatacagca cggaaaaccc ggacctgttg gtgctcgaat
     8821 tccgcaatga tacgtcagca ggggatggcg cgcgcattga gcagtttgat cgcaaaggta
     8881 tggtgaacaa caagttcaac tacttcatta tgagcaaact ggctgaagcg ggtatcccga
     8941 ctcaaatgga gcgtctgctc tccgataccg aatgtctggt gaaaaagctg gatatggtgc
     9001 cggttgagtg tgtcgtgcgt aaccgtgctg ctggctctct ggtgaaacgt cttggaatcg
     9061 aagaaggtat tgagctgaac ccgccactgt tcgatctgtt cctgaaaaac gacgccatgc
     9121 acgatccgat ggtcaacgaa tcttactgcg aaacctttgg ctgggtaagc aaagagaacc
     9181 tggcacgcat gaaagagctg acctacaaag cgaacgacgt gctgaaaaaa ctgttcgatg
     9241 atgctggtct cattctggtc gacttcaagc tggagtttgg tctgtacaaa ggtgaagtgg
     9301 tactgggcga tgagttctcc ccggacggca gccgcttgtg ggacaaagaa acgctggaga
     9361 aaatggacaa agaccgtttc cgccagagcc tcggtggcct gatcgaggcc tatgaagccg
     9421 tcgcccgccg cctgggtgta cagctggact gatttttctg ttcatcatct tgccgtgcag
     9481 ctggcacggc aagacaaccg ctctcataag atgagtattg aacgctcatt cctccgccat
     9541 ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc tacgatcatt tctataatga
     9601 atatattgtt gaggtatcta tgcgttggca agggcgacgt gaaagtgaca atgttgaaga
     9661 caggcgcaac agctctggtg gtccgtcaat gggcggtcca ggttttcgcc tgccaagcgg
     9721 taaaggcggg ctgattttac tgatagtcgt gctggttgca ggctactatg gcgttgattt
     9781 aaccgggttg atgaccggac agccggtttc ccaacagcaa tcaacacggt caattagtcc
     9841 taatgaagac gaagccgcaa aattcacttc ggtgattctg gcaaccacgg aagacacctg
     9901 gggacagcaa ttcgagaaga tgggtaaaac ctatcagcaa ccgaagctgg tcatgtaccg
     9961 tggaatgacg cgcaccggct gcggggcggg ccagtccata atgggaccgt tctattgccc
    10021 ggcggatggc acggtttata tcgatctctc cttctatgat gacatgaaag acaaacttgg
    10081 cgcggatggc gattttgccc aggggtacgt tatcgcccat gaagtcggtc atcatgtgca
    10141 gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa caaaacgcga cgcaggcgga
    10201 agtgaaccgc ttatctgtgc gtatggaact ccaggccgac tgttttgccg gtgtctgggg
    10261 gcatagtatg cagcagcaag gtgttctgga aaccggcgat ctggaagagg cgctgaacgc
    10321 ggcgcaggcc atcggcgatg accgtttaca acagcaaagt caggggcgag tagtaccaga
    10381 cagtttcact catggcactt ctcagcaacg ctacagctgg tttaaacgtg gtttcgacag
    10441 cggcgatccg gcacaatgca atacttttgg taaaagcatt taacttccgg ggcagggatg
    10501 gctgaactga ctgcgcttca cacattaaca gcgcaaatga aacgtgaagg gatccgccgc
    10561 ttgctggtgt tgagcgggga agagggttgg tgttttgatc atgcgcttaa gttgcgtgat
    10621 gccttacctg gcgactggct gtggatttcg ccgcagccag atgctgaaaa ccactgttct
    10681 ccctcggcgc tacaaacttt acttgggcgc gagttccggc atgcggtatt cgacgcccgc
    10741 cacggctttg atgccgctgc ctttgcggca cttagcggaa cgttgaaagc gggaagctgg
    10801 ctggttttgt tactccctgt atgggaagag tgggaaaacc aacctgatgc cgactcgctg
    10861 cgatggagtg attgccctga ccctattgcg acgccgcatt ttgtccagca tctcaaacgc
    10921 gtacttacgg cgaataacga cgctatcctc tggcggcaga accagccgtt cacgttggcg
    10981 cattttactc cccgtactga ctggcacccc gcgaccggcg caccacagcc agaacaacag
    11041 caactcttac agcagctact gaccatgcca ttgggcgtgg cggtggtaac ggctgcgcgt
    11101 gggcgcggta aatcggcgct ggcagggcaa ctcatttctc gtattgcggg tagtgcgatt
    11161 gtcaccgcgc ccgcaaaagc ggcaacgtat gtactggcac aatttgcggg cgagaagttt
    11221 cgctttattg cgccggatgc cttgttagcc agcgatgagc aagccgactg gctggtggtc
    11281 gatgaagccg cagccatacc tgcgccgttg ttgcatcaac tggtatcgcg ttttcctcga
    11341 acgttgttaa ccactacggt gcagggctac gaaggcacgg gacgtggttt tttgctgaaa
    11401 ttttgcgctc gctttccgca tttacaccgt tttgaactgc aacagccgat ccgctgggca
    11461 cagggatgcc cgctggaaaa aatggttagt gaggcactgg tttttgacga tgaaaacttc
    11521 acccacacac cacaaggcaa tatcgtcatt tccgcatttg aacagacgtt atggcgaagc
    11581 gagccacaaa cgccgttaaa ggtttatcaa ttattgtctg gtgcgcacta ccggacttcg
    11641 ccgctggatt tacgccgcat gatggatgca ccagggcaac attttttaca ggcggctggc
    11701 gaaaacgcga ttgccggggc gctgtggctg gtggatgagg gcggattatc tcaagaactc
    11761 agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta atctggtggc ccagtcgctg
    11821 gcggcgcacg gcagcaatcc actggcggcg acattgcgcg gaaggcgggt tagccggata
    11881 gcagttcatc cggctcgtca gcgggaaggc acaggtcagc aactgattgc tggtgctttg
    11941 caatatacgc atgacctcga ctatctttcg gtgagttttg gttacaccgg ggaattatgg
    12001 cgtttctggc aacgctgcgg ttttgtgctg gtgcgaatgg gtaatcatcg tgaagccagc
    12061 agcggttgct atacggcgat ggcgctgtta ccgatgagtg atgcgggtaa acagctggct
    12121 gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc tcgcaaagtg gaatggcgaa
    12181 acgctccctg ttgatccact aaacgatatc gtgctttctg ccgacgactg gcttgaactg
    12241 gccggttttg ctttcgctca tcgtccgcta ttaacgtcgt taggttgctt aatgcgtctg
    12301 ttacaaacca gcgaaatggc attaccggcg ctgcgtgggc gtttacagaa aaacgccagc
    12361 gacgcgcagt tatgtaccac acttaaactt tcaggccgta agctgttact ggtccgtcag
    12421 cgggaagagg ccgcgcaggc gctatacgca cttgatgatg ttcgcactga gcgtttgcgc
    12481 gatcgcataa cgcaatggca attttttcac tgactccttc aattttctcc catggtcatt
    12541 gtgagtcagc ggcgtaaaat taatgacatc agttaaggag ttcgccatga aacatgacca
    12601 ttttgttgtt caaagcccgg ataaacctgc gcaacagttg ttactgcttt tccacggtgt
    12661 cggggataac ccggtggcaa tgggggaaat aggtagctgg tttgcgccgc tatttcctga
    12721 tgcgctggtg gtgagtgtcg gaggcgcgga gccgagcggt aatccggcgg ggcgtcagtg
    12781 gttttcggtg cagggtatta cggaagataa tcgccaggcg cgtgtggatg ccatcatgcc
    12841 gacgtttatt gagacggtgc gctactggca gaaacagagc ggagtagggg ctaccgccac
    12901 ggcgttaatc ggtttctctc aaggcgcgat tatggcgctg gagagcatta aagccgaacc
    12961 gggccttgcc tcacgcgtca ttgcctttaa cggacgctat gcgagcctgc cggaaactgc
    13021 gtctacagcc acgacaattc acctgattca tggcggtgaa gatccggtta tcgatctggc
    13081 gcatgcggtt gcggcgcagg aagcgttaat cagtgccggt ggtgacgtga cgctggatat
    13141 cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg caatttgctc tggatcactt
    13201 gcgttatacc attccgaaac attatttcga cgaagcgcta agcggcggta agcctggcga
    13261 tgatgatgtg attgaaatga tgtaataata gccgggtagg tctgataaga ctgtatcagg
    13321 catcatggcc ggatgcggcg ttaacgcctt atccggccac ttagcttact tcttcggttg
    13381 atcctttttc ggccagtcgt cgtcatcgtc ccacttatcg ttgaaatcac gatgcggggg
    13441 aagctccggc ttgttggcga gaaatttctt atggttgacg cgcttgagat ctttaatcac
    13501 gttcagcagg acgcctacca aaaagacaat caccagaatc caccaatatt tagccagcca
    13561 gtccatgctt atttcctctt gcagaaccac tcatcaggcg acgagctgtt ccatgatacg
    13621 ttgatacata cgggcaagta gctgcaggtc ggcagcgttc acacattcat taattttatg
    13681 aatagtggca ttgaccggcc cgagttccac cacctgcgcc cccatgcggg caataaagcg
    13741 cccgtcggac gttccgcctg tggttagtag ctgcggttta atttcattat agtgctcaac
    13801 cgcgttaacg accgcatcca ccagtttacc gcgcgcggtc aaaaatggct gcccggaaag
    13861 ccaccaatcc accgtatagc gcagttgatg tttttcaagc agggcaagta cctgcgcttt
    13921 gatcatctca tcagtcagtt cggtgctgaa gcggaagtta aactgcacaa acagttcacc
    13981 cggaataacg ttgttactgc ccgttcccgc ctgaatattg gcaatctgca tactggtcgc
    14041 cgggaagaat tcattgccct gatcccactc aatagccact aattcattaa ggaaaggtgc
    14101 tgcgcgatgt accggattgt cagccagatg tgggtaggca acatgcccct gcacgccatg
    14161 aatggtgagg ttgcaggtta acgatccgcg acgaccattt ttcaccacat caccaaccac
    14221 ttcgatactc gacggttcgc caaccagaca gtaatcgaga cgctcattac gcgccattaa
    14281 cgcttcgacg acttttaccg taccgttgtg ggcactagct tcttcatcag aggtgatcag
    14341 aaatgccagt cgccccgtat ggttgggatg ttgtgcgaca aaacgttctg ccgctaccac
    14401 catcgccgcc agcgagcctt tcatatctgc cgcaccgcgc ccgaataaca tgccgtcacg
    14461 aatggtgggt tcaaacggcg gattgatcca acggtcggcg tcgccaggcg gcaccacgtc
    14521 ggtatgcccg gcaaaggcta acgtttcacc ctgcccacgc catgcccaaa aattctgcgt
    14581 atcggcaaag tccatgcgtt caacggtaaa accgatcgcc tgcaaacgtt caatcaacaa
    14641 agcctggcat cctgcgtcat caggactcag ggaagggcgg cgaataagct gttgtgtcag
    14701 ctcaataacc gggcacgaca tagactacac ctcatggaaa aattgctgat aactggattc
    14761 actgaaaccc agcagcatag gcttaccagg cgcgcagagc aatggacgtt tgataattgc
    14821 aggcatttca gtcattaatg tcgccgcaga ggccgcatcg gtgattttat tgcgggtggt
    14881 ttcgtccagt ttacgccagg ttgtaccacg ggtgttgagt aacgcttccc agcctaattc
    14941 gttgataaaa ccgttcaata attcgctgtc caatccatcg acgcggtaat catgaaagcg
    15001 atagtcgatg ttattggctt ccagccaacg gcgagccttt ttaatggtgt cacaattttt
    15061 gatgccgtaa agtgtaacca taatgaatcc tttttcgatt tatgagaata aa
//
LOCUS       DAZUCC010000123        14921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZUCC010000123 DAZUCC010000000
VERSION     DAZUCC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.124"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(156..377)
                     /gene="kil"
                     /locus_tag="ACHY09_003185"
     CDS             complement(156..377)
                     /gene="kil"
                     /locus_tag="ACHY09_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560216.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS3886126.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIEKRKLYIR
                     SLTTKTCITNRMIKVFIGRDGLPVKAESL"
     gene            836..1045
                     /locus_tag="ACHY09_003186"
     CDS             836..1045
                     /locus_tag="ACHY09_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886127.1"
                     /translation="MTQEEKVMFLMRLAVDTYNTQFREKDIPQKAVPAAVDKGGAIAV
                     FYDAFESFFDEKLDAVSDFGTSSNK"
     gene            complement(1014..1373)
                     /locus_tag="ACHY09_003187"
     CDS             complement(1014..1373)
                     /locus_tag="ACHY09_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886128.1"
                     /translation="MNETELKHIIAMLLEDARLVYRLSPNSATQERIRMAETALKQDN
                     GNGISKFSAASENEVIEINSNISDSCVAYSHQIIRVSARIMEVMASELEKNNIKPTDC
                     CLRTVMNVIYYSMFRSR"
     gene            complement(1405..2118)
                     /locus_tag="ACHY09_003188"
     CDS             complement(1405..2118)
                     /locus_tag="ACHY09_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886129.1"
                     /translation="MTTPSVLPQKLWRPLAEIKNFVEKMPDGVRLTEVTKKVKTFAEL
                     SGKERNQLIDFIDKRESIIVFKVRKEGSGNGVTFFRHKKYGYPKREGNVTIIKDLQSK
                     LCTRCGQTKSVDDFYSDASKRDGRAIYCKKCESAMKRSRRECNKLILQQQEPEMNNLK
                     AVSPSPEILRKQAEELLKAAEIAEKKRQEDDVFNKKLAPLKLEILQAAGKMQLKLDEF
                     IDCMDEMNKAVQKLKELTA"
     gene            complement(2122..2394)
                     /locus_tag="ACHY09_003189"
     CDS             complement(2122..2394)
                     /locus_tag="ACHY09_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFS3886130.1"
                     /translation="MSRKTEFKGTATSRRRARRAELQSLEASSAEKLHRPTPSRVYLQ
                     CKRKAAMRAEVVTITTLTRKYEGSTCLPNTALYAAGYRKSGTITAR"
     gene            complement(2372..2598)
                     /locus_tag="ACHY09_003190"
                     /pseudo
     CDS             complement(2372..2598)
                     /locus_tag="ACHY09_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2843..3619)
                     /locus_tag="ACHY09_003191"
     CDS             complement(2843..3619)
                     /locus_tag="ACHY09_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HFS3886131.1"
                     /translation="MGNVTCSTVISKLGFECHPMSDTLLRVISPFTYYDDCEQISVFV
                     QEMSGQYRVTDYCDTLMNIESRGIHLTKKKIDLIRSSLASQGISLNDSGEISAWADES
                     SVGQVTANVIRGGILASAQTADWYAEVKDDKFEKCVISYLKSAGLGKRLALKEKVKGI
                     SGHNITVPLTLRNESRLIPPKRGFTVSLASSKGWNTAHSTVGKIVDLSQVVPDINNRF
                     VIVDSDGLTPELQQLSLLFNDTARVLPFHTRDSWIESLVA"
     gene            complement(3607..4149)
                     /locus_tag="ACHY09_003192"
     CDS             complement(3607..4149)
                     /locus_tag="ACHY09_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886132.1"
                     /translation="MAKKGDFKPTQKEVDQAITRLKKVTFSGVTWTGSEGRTPIWFKL
                     DLKAFDEIGNPITGIRFMLHWRPPIVEGVDIVKISFVMFLHDRRIYALDPYPADNKPH
                     RNKYIVNHPDFVEVARGPHYHMYFESAGEEIALKLETNIKPDDFFGYWNYFCEALNII
                     YEGSPPLPNQDKSGQLSWEM"
     gene            complement(4247..4954)
                     /locus_tag="ACHY09_003193"
     CDS             complement(4247..4954)
                     /locus_tag="ACHY09_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFS3886133.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGVNDEDTLSFVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTVEGHNMIKILGYDRDGEYQFTSINQDHRPITLAYHEVAKVEYVAGILK
                     QSRHLDDIEAREWLKSS"
     gene            5032..5259
                     /locus_tag="ACHY09_003194"
     CDS             5032..5259
                     /locus_tag="ACHY09_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3886134.1"
                     /translation="MQNLDEPIKSVGITEVAKACGVSERAVYKWLKNGFLPKTEFFGK
                     TKYASKIEEISGGRYQASELLEISKKNLLAA"
     gene            5398..5694
                     /locus_tag="ACHY09_003195"
     CDS             5398..5694
                     /locus_tag="ACHY09_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFS3886135.1"
                     /translation="MSQTSYSKLSQRDIDRAETDLLINLSALTQRGLAKMIGCHESKV
                     SRTDWRYIAAILCAFQMASDISPISRAFQHAINVHANKKRPVGPGRSEQILMNI"
     gene            5757..6188
                     /locus_tag="ACHY09_003196"
                     /pseudo
     CDS             5757..6188
                     /locus_tag="ACHY09_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            6618..7358
                     /locus_tag="ACHY09_003197"
     CDS             6618..7358
                     /locus_tag="ACHY09_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS3886136.1"
                     /translation="MKNISTGGILERVRRLAPPHVAAPFRTTDEWREWQLAEGRKRSE
                     EVNRQNHQTRVEKILNRSGIQALHRKCSFGNYRVQNDGQRHALSQAKSIAAELHTGCT
                     NFVFSGKPGTGKNHLAAAIGNWLMAKGRSVIIVTVSDVMSVLHDGYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNELVTLNQIVDRRTASLRSVGMLTNLNHAAMSTLLGERV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            7384..8153
                     /locus_tag="ACHY09_003198"
                     /pseudo
     CDS             7384..8153
                     /locus_tag="ACHY09_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403602.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            8168..8599
                     /locus_tag="ACHY09_003199"
     CDS             8168..8599
                     /locus_tag="ACHY09_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFS3886137.1"
                     /translation="MAKVFTPEQREELKARIVELVRLNGRGTFRQLATETGVSKTAVR
                     RLSRELVVSGAIFDSGYGLFPSDQARKDWQNARKKLSRAKAKKSVVVDPGLIRALPDG
                     EIRRYDRRYNTICRECRNSETMQRVLAFWRGSAEGLMFSPS"
     gene            complement(8632..9306)
                     /locus_tag="ACHY09_003200"
     CDS             complement(8632..9306)
                     /locus_tag="ACHY09_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFS3886138.1"
                     /translation="MGLVSFDITCPHCLKERAVIEAFAEKQIEKTPLFNVAFVCRSCH
                     RGGVAIVEIPSDHYHGPMAESQKKDLDILISGNSQYRFRKIYPAVKKITAPEYTPPVA
                     DRTFVEAKEDLQRRRYDTVVILCRRVLDISTKKLLGDEAGKESLSQRIQMIYKKGLIT
                     EQMKEWAHIVRIDANKAVHTDEVFTPIEASQILSFTEMFLVYAFTLPAMVAARREQKR
                     SDSASI"
     gene            9305..9550
                     /locus_tag="ACHY09_003201"
     CDS             9305..9550
                     /locus_tag="ACHY09_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886139.1"
                     /translation="MDIPREIKEINEIIEVTDRPEFTLMRRYETGTDEQKYIMVAALA
                     VMAIERERREKDVMVIPARNDSPDLRWQELEWDVAIR"
     gene            complement(9598..9903)
                     /locus_tag="ACHY09_003202"
     CDS             complement(9598..9903)
                     /locus_tag="ACHY09_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFS3886140.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NATISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(9993..10265)
                     /locus_tag="ACHY09_003203"
     CDS             complement(9993..10265)
                     /locus_tag="ACHY09_003203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886141.1"
                     /translation="MSLAAPIRDPIMNIIARFFYLIIFCMATSGCTTTTKINRGDEKE
                     QYIIACGAATPWGVCYDKANALCKNGYKDILKEQGFNRKELTIECK"
     gene            complement(10319..10576)
                     /locus_tag="ACHY09_003204"
     CDS             complement(10319..10576)
                     /locus_tag="ACHY09_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFS3886142.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAMEAWLIHSAAPPLDAIRENPGKGHSISQVRSEIRARK"
     gene            10904..11209
                     /locus_tag="ACHY09_003205"
                     /pseudo
     CDS             10904..11209
                     /locus_tag="ACHY09_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            11478..12140
                     /locus_tag="ACHY09_003206"
     CDS             11478..12140
                     /locus_tag="ACHY09_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFS3886143.1"
                     /translation="MTMQLAVESLVVVTYSNVPVITTGLLANLYKTEEKHIRQNFKRN
                     ECRFIAGKHFFKVSGCELDSLRTSQRGLQISPKTRSLILWTERGAARHAKMLETDQAW
                     EVFEKLEDCYFSQGKTTQTEQQPQIQPQFTAEEIILLCYMQLWMEKAQDLSKHLYPIM
                     KELNSSCTNKLYDIAFETIYMVTKNRDALLREVTRLDMSSSVIQRAMPMLKSLRARQF
                     EF"
     gene            12195..12605
                     /locus_tag="ACHY09_003207"
     CDS             12195..12605
                     /locus_tag="ACHY09_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFS3886144.1"
                     /translation="MNQTIFLRSKQQQQFAINAILATTLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMVADISRQVQWYGRWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTSWAVEQGIRFTDRRYE"
     gene            12602..12793
                     /locus_tag="ACHY09_003208"
     CDS             12602..12793
                     /locus_tag="ACHY09_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFS3886145.1"
                     /translation="MRRKRRSITDIVCENCNYLPTKRSRNKPRPIPNESDVKTFNYTA
                     HLWDVRWLRRRARFNSHSG"
     gene            12817..13107
                     /locus_tag="ACHY09_003209"
     CDS             12817..13107
                     /locus_tag="ACHY09_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFS3886146.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLLAAWACSDCHNEIDRRTRILDNNDARLYHLEGVIRTQAILLKEGKIKS"
     gene            13104..13466
                     /locus_tag="ACHY09_003210"
     CDS             13104..13466
                     /locus_tag="ACHY09_003210"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS3886147.1"
                     /translation="MNEYEFVLPWPPTVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LRLDIFTKSRLRITIIAEPPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRVGIKITELENI"
     gene            13466..13660
                     /locus_tag="ACHY09_003211"
     CDS             13466..13660
                     /locus_tag="ACHY09_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFS3886148.1"
                     /translation="MNAKIQTIPELLICTRGNQTEVARILNCNRATVRKYIDDKDAKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            13653..14033
                     /locus_tag="ACHY09_003212"
     CDS             13653..14033
                     /locus_tag="ACHY09_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFS3886149.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKELIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDGTIGKKLQKA
                     EGVVEGMLMLLGVRLEMDRYVQRL"
     gene            14165..14755
                     /locus_tag="ACHY09_003213"
     CDS             14165..14755
                     /locus_tag="ACHY09_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886150.1"
                     /translation="MDIEDFLEEENDLALWNDDILSIDWFIILKELLKYQHRLKLNHS
                     ELLLLANFVSFHQDADSQTLPSISLFATRMRTSRDTIQGVLVRLEEKDMLQKISYAQI
                     DHGDDLRNIYDIKPLIRRLIWLLKSGCDKKHTCPLCGKVAISNEEIEKKFGFRICGNK
                     KRPQSWCRSCRSPKQRRLNTLLCGKKRARKSSPEVV"
BASE COUNT         4044 a         3375 c         3551 g         3951 t
ORIGIN      
        1 tttctttgag ttcatttatt gcatcagggc tggtttcgga aaatatttca ttttgcgcaa
       61 gttctttcat tttgattttt ccgtgattga taattaatgc ggtttttatt tcggcttgtc
      121 tgttacagat atggaaggtg tattttaatt actcgtcaca acgactctgc ttttacgggt
      181 aagccatcgc gcccgatgaa tactttaatc atgcggttgg taatgcatgt ttttgtagtc
      241 aggctacgaa tatagagctt tctcttttca atattgtttg ctgaagcgat atatgttcgg
      301 ccttcatgaa gaacataatc gccaggtgtc acgcactgac gtggtatttc atcagttccg
      361 aagtgatgag caatcataat tatctccatt ttcacaaatg aactttgttg atgcggtgcc
      421 tggtgcctcc aggtgacgtt aaccagttaa caattaacgc cggctcaggg gacgatgact
      481 ttccgtgaca tcctgtcggt ttaactgttc cgcgtgcgct gagccgcatt taccgcatca
      541 caagattcac tttaaaaagg gcggacatca gccagcaatt aaactgatgc cgccaatgac
      601 tacacacagc aatgttgtta ttcacaaccg gaagtgcacg gtcgaagaaa tctaacgaca
      661 agccttcaaa gggaaagagt cttcgccgtg cgttttcgtg ttgtaccctg gcttttcagg
      721 gtaatatctg ttcagtaaac tgacagtgcc ggatgctcac ccgtgtccgg cgcacgaact
      781 ccacctgacc cgtggagaac tcctcaacta caaaccccgt aaggagagtg aatttatgac
      841 acaagaagaa aaagtgatgt ttctgatgcg gctggctgtc gatacctaca acacacaatt
      901 cagggagaaa gatatacctc aaaaggcagt tcctgccgcg gtagataagg gcggtgctat
      961 tgccgtattt tacgatgcat ttgaatcatt ttttgatgaa aaactcgacg ctgttagcga
     1021 cttcggaaca tcgagtaata aataacgttc attacggttc ttaaacaaca atcagtgggc
     1081 ttgatgttgt tcttttcaag ctcacttgcc atcacttcca ttattcttgc acttacacga
     1141 attatttggt ggctgtaagc gacgcaactg tcgctgatat tgctgtttat ttctattact
     1201 tcattttcac tggcggcgct aaatttggat atgccgtttc cattgtcctg tttcagtgct
     1261 gtttctgcca tccggatgcg ttcctgcgtt gcggaatttg ggctaagtcg ataaaccagt
     1321 ctggcatctt ccagaagcat ggcgataata tgcttcagtt ctgtttcgtt catagttaac
     1381 tccggtagtt gcaatttatt aatatcaggc ggtcagctct ttaagcttct gaactgcttt
     1441 attcatttca tccatacagt cgatgaattc gtccagttta agctgcattt ttccggcggc
     1501 ctgaagaatt tcaagtttta agggcgcaag ttttttgttg aatacatcat cttcctggcg
     1561 ttttttctcc gcaatttcgg ctgctttcag caattcttcc gcctgctttc tgagtatttc
     1621 tggtgaaggt gaaactgctt ttaggttatt catttcaggt tcctgttgtt gcagaattaa
     1681 tttgttgcat tctctgcgtg agcgtttcat tgcagattcg cacttcttgc aataaatggc
     1741 tctcccgtca cgtttgctgg catctgaata aaaatcatcg actgattttg tctgcccgca
     1801 tctggtacat aattttgatt gaaggtcctt aatgattgtg acgtttcctt cccgtttggg
     1861 atatccatat tttttgtggc ggaaaaaggt tactccgtta ccagaacctt cttttctgac
     1921 cttaaataca atgatgcttt cccgtttatc gataaaatcg atgagctggt ttctctcttt
     1981 tcctgacagt tcggcaaacg tcttaacttt ttttgtaacc tccgtaaggc gaacaccatc
     2041 aggcattttt tcaacgaagt ttttaatctc tgcaagcgga cgccataatt tttgcggcaa
     2101 aactgaaggc gtggtcatca atcacctcgc tgttattgtt ccggatttgc gatagccagc
     2161 tgcgtaaagt gcagtgtttg gaagacaagt agagccttca tattttctgg ttaatgtcgt
     2221 tatcgtgact acttcagctc gcattgcggc tttgcgcttg cactgtaagt acacccgtga
     2281 aggcgtgggg cggtgcagtt tctcagcgct tgatgcctcc agactctgaa gctcagcccg
     2341 gcgtgctctg cggcgggaag ttgcggttcc tttaaattct gtctttctgg acatagactt
     2401 ctctccgaat aaactctggt gatgcaatct cgaagcctcc cctgagatga ttgcttcggc
     2461 attgcacccg acagcttatg cggttgggcg atctggcttt gcagccacgt agtcgagtgt
     2521 tcgacgttgt ttaaagagcc tgccagtctg ttccgtttgg cttccagcgt cctgctgatg
     2581 gtgataataa tgaactattg gttcgagata atcaagaacc acaagtacgg aatttgttgt
     2641 ttattcgttt tcccgagata tggttttgat atataaggta ttttattttt gtaaatgttg
     2701 aggatgaagg tgatggggca gggatcagaa ttgcactgta tagcaagttg tatctataaa
     2761 tttttcaata aatacaattg gttatgtttt ttaggtgggc gatcgtgagg caaagaaaac
     2821 ccggcgctga ggccggtttt ttctaggcta ccagagactc aatccaagag tctctggtat
     2881 ggaatggcaa cactcgtgcg gtatcattaa atagtagtga tagttgctgt agctcaggtg
     2941 ttaacccatc gctgtcaact attacaaatc tattgtttat gtcaggaacg acctgactta
     3001 agtcaacaat cttcccaact gttgagtggg cagtattcca tcccttactg ctggcaaggc
     3061 ttaccgtaaa cccgcgtttt ggtggtatta gtcgagactc attccttagc gttaacggaa
     3121 cagtaatgtt atgcccacta atacctttca ctttttcctt taaggctagt cgcttcccaa
     3181 gccctgctga ttttaagtaa ctgattacac atttttcgaa cttatcgtct ttgacctcag
     3241 cataccaatc agcagtttgg gcggatgcaa gaatcccacc acgaataaca tttgcagtta
     3301 cctgtccaac ggacgactca tctgcccacg cagatatctc tccagagtca tttaatgaaa
     3361 ttccttgcga agcgagtgat gacctgatca gatcaatttt ctttttagtc aggtggatgc
     3421 cgcgtgattc aatattcatc aatgtatcgc agtagtctgt aaccctatac tgaccactca
     3481 tctcttgaac gaatacactt atctgctcac aatcatcgta gtatgtgaag ggactaataa
     3541 cgcgcagcaa cgtgtcgctc attgggtggc attcaaaccc gagcttagat atgactgttg
     3601 aacacgttac atttcccatg ataactgacc tgatttatct tgattaggta aaggtgggct
     3661 gccttcatat atgatattaa gcgcctcgca aaaataattc cagtagccaa aaaaatcatc
     3721 tggcttgatg ttcgtttcaa gcttaagtgc aatttcttcc ccagctgatt cgaagtacat
     3781 gtgatagtga ggacctcgag ccacctcaac aaaatctgga tggttcacta tatatttatt
     3841 acggtgtggc ttgttatccg cagggtacgg gtcaagcgcg taaatgcgcc tgtcatgaag
     3901 aaacatcaca aatgaaatct tcactatatc caccccttca acgataggag gacgccagtg
     3961 aagcataaat cttatgcctg tgattgggtt gccaatttca tcaaaagctt tgagatccaa
     4021 tttaaaccag attggggttc gtccctcact tccggtccat gtaactccgc taaaagttac
     4081 ttttttcaag cgagtaatag cttggtcaac ctctttctgg gtaggcttaa agtcgccttt
     4141 tttagccact gattcgtatc accatgaaag ttattgttaa tgcctgtgct tcttgtcacc
     4201 caaacgtctc ttcaggccac tggttaccag ctatgtgacg atgaggtcac gaacttttca
     4261 gccattccct tgcctcgatg tcatccagat ggcgagattg cttcagaata ccagctacat
     4321 actcaacctt tgctacttca tgataagcca acgttattgg cctgtggtcc tggttgatgc
     4381 ttgtaaattg gtattctccg tctctgtcat agccaagaat cttgatcatg ttgtgccctt
     4441 caacggttct gacaaacacc tcatcacccg ggaatacttt ggtgttaggc tcaatgagta
     4501 catattctcc tgattttatt ctgggccaca tgctgtctcc tttcacacga agaccaaagg
     4561 catctggatc atcgctataa atcttgagcc acccatcgcg ctcttcggtc atctcgatgg
     4621 caccatcaac accaagaata gcctcaccaa ccacgcgcac taaccctttt tttaatttgc
     4681 caacaaatga aagagtatct tcatcattca ctccatttaa cgaagtgcca tgctgaagcc
     4741 aaacaacatc aacgtttaga aatttcgcga gcgcattcat tttttcctga cgcggtaaag
     4801 actcagcatt aaaccatttg ctaactcctt tggacgaaag agaaagagca cgggctatag
     4861 ccatccccct accatgttca tcaagaccag cttctttaca ggcttgcgct agccgctggg
     4921 cgaattcttt gcgcactttt tcattttgaa ccatgagtac gataataaag cacttgcaaa
     4981 aactttcagt tcaaccataa tgcgtactta aagtacgaaa gaggtatacc tatgcaaaat
     5041 cttgatgagc cgattaagag tgtcggcatc actgaagttg cgaaggcttg tggagttagc
     5101 gaaagggctg tctataagtg gctcaaaaac ggcttcctcc ctaagactga gttttttggg
     5161 aaaacgaaat acgcctcaaa aatcgaagag atttctggtg gcagatatca agcaagcgaa
     5221 ttgcttgaaa taagcaaaaa gaaccttctg gcagcataag taaccccgct ctttgtaaca
     5281 acggacattc gtcctacgtc gctgaaaagc gagtcctaag atatctgacc aactaaggcc
     5341 atatgcgttt ccacgcatac ctttcaacta actattcact atttggaatc ataagaaatg
     5401 tcacaaacaa gttacagcaa actgtcgcag cgcgatatcg atcgcgctga aacggattta
     5461 cttatcaacc tgtcagctct gacgcaaagg ggactggcga agatgattgg ctgccatgaa
     5521 tcgaaggtca gtcgtaccga ctggcgatac atcgcggcga ttttatgtgc gttccagatg
     5581 gcatctgata tcagtccgat cagccgggct ttccagcatg ccattaacgt tcatgcaaat
     5641 aaaaaacgtc cggttgggcc cggacgttct gagcagatcc tgatgaacat ctgatattca
     5701 ggcaggatat aaagcaacat acagggggaa ttatgccata tccggcagaa aatacaatct
     5761 cacattattg tgacaacgta acaccaatca ggcctgcctt gcaggccgtg gagtgtcatg
     5821 tggcagatct cgatgatggc tatgtcagac tatcaaatat gctgcttgag gcttattccg
     5881 gcgcagattt gaccaagcga cattttaaag tgctgcttgc cattctgcgt aaaacttatg
     5941 gctggaataa gccaatggac agaatcacag atgctcaact tagcgagatc acaaagttac
     6001 cagtcaaacg gtgcaatgaa gctaagttag aactcgtcag aatgaatatt ctcaggcagc
     6061 aggggggcat gtatggaccc aatgcaaaca tctcagaatg gggtatccct caagagggaa
     6121 catcccctaa aacaggggat aaaacatccc ctaaaacagg ggataaaaca tccctcaaaa
     6181 caggggataa aacatccctc aaaatgaggg aacacaaaag acataaagac aataaagaca
     6241 taaaaacatt aacccatccc aaacccttcc cttccggaag ggatttttgt ggtgatggaa
     6301 aattcaatcc tgccgatgtt gaacttcccg actggattga accgtcgctc tgggtatcct
     6361 gggttgacta ccggcagaac atccgaaaac cgatcaaatc ccctcagacc gttacccagg
     6421 cgctcagact ccttgaccgt gctcgccaga taggctactg gcctgaagaa attatcaatc
     6481 gcagtatcgc caacggttgg cagggattgt tcataccgaa cgaatctcgc cgtgagccgg
     6541 agcagcgccg cagttgtgtg aacatgattt ctgagccgga taccgcgatt cctacggggt
     6601 tcagggggta atcagccatg aaaaacattt cgacaggagg gattcttgaa cgggtgcgcc
     6661 gtctggcacc accgcacgtg gcagcaccgt tccggacgac cgacgaatgg cgggaatggc
     6721 aactggctga gggccggaag cgcagcgagg aagttaaccg ccagaatcac cagacgcggg
     6781 ttgaaaaaat cctgaatcgt tcgggcatcc aggcgcttca caggaagtgc tcattcggga
     6841 actaccgggt gcagaacgac ggtcagcgcc atgctctgag tcaggcaaaa tccatcgcgg
     6901 cagagctgca taccggctgc acgaatttcg tgttcagcgg taaacctggc accgggaaaa
     6961 atcaccttgc agcagcgatt ggcaactggc tgatggcgaa ggggagaagc gtgattatcg
     7021 tcaccgtgtc cgatgtcatg agtgtgttgc atgacggcta cgacaacggc aagtccggtg
     7081 agaaattttt acaggaactt tgcggggtag accttctggt ccttgatgaa attggcatgc
     7141 agcgggatac gcgcaatgag ctggtcacgc ttaatcagat cgtcgaccgc agaacggcat
     7201 cactgcgcag tgtcgggatg ctgacgaacc tgaatcacgc agcgatgagc acactcctcg
     7261 gcgagcgggt gatggaccgt atgaccatga acggtggtcg ttgggtgaat tttaactggg
     7321 agagctggcg atcaaacgtt ggacgtcagg gtatgtgaga atttttgacg aggtaaattt
     7381 tcgatggaaa ccgtattgca tgcactgaaa gcgatgggaa aagccaattc tgttgaactg
     7441 gcggcgcggc ttgatatcag ccgtgaagaa gttctcaacg aactgtggga actcaaaaaa
     7501 aatggcgttg ttgataaaac gggtcacacc tggtttctgg ctgtcgaagg tgaatccagg
     7561 gtaaccgaag ggcaggcact acaacctgaa gcgccggatg tggtaaccga agaggtcgct
     7621 ccaaaagtta gcgctgacat gatgattgag tttatctctc aggagggggc taaaacctgt
     7681 gaagaaatag cgggtaagtt cggcgttacc actcgtaagg ttgcttccac gttggcggtc
     7741 gtaacagcaa cggggcgcct ggtgcgcgtg tctatcaagg gaaaattccg ttataaagtg
     7801 cctgacgtcg aaaacggcga tttgagcgcg tcatcatcgc aggatgagaa atcaaccacg
     7861 aagcagaaaa cagcgcgtca ggcggcgaac tgaatcgttc tccgttgatg tcagagggca
     7921 tggcaaaggc ctgtacagat gagtttatcc aggatgttcc gtcgttcacc gaaaaatatg
     7981 ctggtgcagt gacccttcca cctctacggg ccgccagtcg tgaacttcgt cgcgccaaaa
     8041 aacaggtcca gaagtgggag cgtgtctgtg ctgcactacg tgagctgaat aaacaccggg
     8101 aacttgtcat gcagattacc gattcatcca gcagtattgc atcggaaaag tgataactgg
     8161 atagcttatg gcaaaagtat ttacacccga acagcgggaa gaactgaagg cgcgtattgt
     8221 ggagctcgtg cgcctgaatg gtcggggaac gttcaggcaa ctggctactg aaaccggagt
     8281 cagcaagaca gctgtcagac gtttgtccag agaactggtt gtcagtggcg ctatattcga
     8341 ctcaggttat ggtttatttc cgtctgatca ggcgcgtaag gactggcaaa atgcccgcaa
     8401 aaaactctcg agagcaaagg caaagaaatc ggttgtcgtt gatcccggtc ttatccgggc
     8461 attacccgat ggggaaatac ggcgctacga cagacgctac aacacaattt gtcgcgagtg
     8521 ccgtaacagc gaaacgatgc agcgtgtgct ggcattctgg cgggggagtg cagagggatt
     8581 gatgttctcc ccgtcgtgaa tgacgggggc ttacgtgttc agaaaagtga ttcatataga
     8641 ggctgaatct gatctttttt gttcacgtct ggctgccacc attgcaggca gagtgaaggc
     8701 gtagactaaa aacatttcgg taaaactcag gatctggctt gcctcaattg gtgtgaaaac
     8761 ttcatctgta tgaactgctt tattggcatc aattcgtaca atatgagccc attccttcat
     8821 ctgttcagtg atcagacctt ttttgtaaat catctgaata cgttgcgaca gcgattcttt
     8881 tcctgcttca tcgccgagca gttttttcgt ggagatatcg aggactctgc gacaaagaat
     8941 aaccactgtg tcgtaccgcc tcctctgtaa atcctctttt gcctcaacaa aagtacggtc
     9001 tgcaaccggg ggcgtatatt ctggtgcggt aatttttttg accgcaggat agatcttgcg
     9061 gaaacggtac tggctatttc ctgaaatcag aatatcgagg tcttttttct ggctttccgc
     9121 catggggccg tgatagtggt ctgatgggat ttcaacaata gcaacacctc ctcgatgaca
     9181 acttctgcat acaaaagcga cgttaaaaag aggtgttttt tcaatttgtt tttcggcaaa
     9241 tgcctcaata acagctcgct cttttaaaca gtgcgggcat gtaatgtcaa aggagactaa
     9301 acccatggat attcctcgcg aaattaaaga aattaacgaa atcattgagg ttactgatcg
     9361 ccccgagttt accctcatgc gtcgttatga aacaggaacc gacgaacaaa aatacatcat
     9421 ggttgcagca ttggctgtga tggcgataga aagggagcgg agggaaaagg acgtaatggt
     9481 tattccggcg agaaatgatt caccggattt gagatggcag gagctggagt gggatgtggc
     9541 aatccggtga ttgatatttc tctggggtaa agcgccgcca gaatgacggc gcggtggtta
     9601 agcatgaggc gaaatttctg ccgggcggta ttttgttgag agatgagata cgtcaacttc
     9661 tctttccgct ttttcaaggc tccatgccgt ctggagacga atccatgaag acggagttcc
     9721 acccaggaca gcagcgagac gaacagccat ttcaggagat attgtcgcat tacctgaaac
     9781 cagtcgctga acagtggaag gcgcgacatc aagcgctctt gccagttccc tgattccaat
     9841 attctgatct tccagaatat cagcaataat ttctccggga tgaggggggt taaacatcgc
     9901 cattagtggt aatcctcgta gttaaacgaa gcggctctgg agttttttcc ctgtagcacg
     9961 ggcgaatttt ttcaacgttg caaatgatgg ccttatttac actcaatggt taattctttc
    10021 ctgttaaatc cctgttcctt taaaatgtct ttatatccat ttttacatag ggcattggct
    10081 ttatcatagc atactcccca tggtgtagcg gcaccacagg caatgatgta ctgttccttt
    10141 tcatctccgc gatttatttt cgtagtggta gtgcatcctg atgttgccat gcagaaaatt
    10201 atcaaataaa agaaccttgc tatgatgttc attatcggat ctctaatagg tgctgcaagt
    10261 gacactattc ccatacctgg agcagtttgt tagtgtggca cttcatatgc catgcagatt
    10321 acttccgggc gcgaatctca gagcgaacct gtgaaatgga gtgccctttg cctgggttct
    10381 cacgaatagc gtcgagtggc ggcgcggctg agtgtattaa ccacgcttcc attgctttgt
    10441 cgcgttcatt cagtgcacga agcccttcac gaataacttc gctttctgaa gcgtatgcac
    10501 cggaagccac acgggcgcgc accatgtcag ccatttcgtt ggttaatgta atgctgaatt
    10561 gttgggttgt acgcatgtta aacctcactg agtaggatag aacactattc gatgatagca
    10621 cgccacttgt tgacgacaac acatatccgg gattatattc ttcgcacgcc agcaaaatct
    10681 ggcgtcggga ttggaacccc ggatagagac cgcgacaaac acacgccgcg ggcgtgtttt
    10741 ttattgtcgt atgcacgcgc acatctgaat tatggtgggg cgcatggggg agctgaaaag
    10801 ctcgccggtc ggtttcccgg tagttccaac cctgtgcgtc tcaccacccg atgattggaa
    10861 ccttacggtg gtgatagttt agaaaccact agagggcgtc attatgacaa ctcaaatctc
    10921 tgttgaaacc atctccccga ttacccataa ccagattcct gttattacca ccgagctttt
    10981 ggcacaactt tatggcaccg aaactaataa catcaaagtg aactatacac gcaatgccga
    11041 gcgttttgtt tgtgggaaac actacttcaa attggaaggg gctggattgc gggaatttaa
    11101 gaacaaggtt actcaaagta acttagttgc accgcgtaca aagcacctca tcctctggac
    11161 agaacgcggc gctgcccgtc atgcaaaaat gctcgaaacc gatcgggctt gacgttatag
    11221 gtcgttactt gtagatttat ctttgtgagg cgtcagaacc tctctaaagc ggacaaaacc
    11281 aaccccgtca gtgttggatt ttttatgcct gttattcagt gatagcacaa tgtgctgtca
    11341 catccccgaa caatgtcggg agggcgacga atacaacacc cgtaagggga ataagtccgc
    11401 ggttctttag gccgttctga acctcccgac accactatca gaggtggtaa tttcagaaaa
    11461 agctaaagga ggtcgttatg accatgcaac tcgctgtaga atccctcgtc gtcgttactt
    11521 actctaacgt ccctgtaata acaacagggt tattggcaaa tctatacaaa actgaagaaa
    11581 aacatatacg gcagaacttc aaacgtaatg aatgtcgttt cattgcagga aaacacttct
    11641 ttaaagtaag tggctgtgaa cttgatagtt tgcgaacctc tcaaagaggt ttacaaattt
    11701 ccccaaaaac ccgctccctc atcctctgga cagaacgcgg cgctgcccgt catgcaaaaa
    11761 tgctcgaaac cgatcaggca tgggaagtgt tcgaaaaact ggaagactgc tatttcagcc
    11821 agggaaaaac aacacaaacc gaacagcagc cgcagattca gccgcaattc acagccgagg
    11881 aaatcatcct cctttgctac atgcagctct ggatggaaaa agcccaggac ctcagcaaac
    11941 acctgtatcc gatcatgaaa gagctgaact catcatgcac gaacaaactg tatgacattg
    12001 cattcgagac catctacatg gtgacgaaga acagagacgc actactgagg gaggtaacac
    12061 gtctcgacat gtcaagttcc gttatccaga gggccatgcc aatgctgaaa agcctgcggg
    12121 caagacaatt tgaattctga aactaaagga gcttcggctc cttttttgtt gggaaaatcc
    12181 aatgagaggg aataatgaac cagactatct tcctccgaag taagcagcag caacaatttg
    12241 ccattaacgc catccttgca acaactctcg ataaagacaa acccgttacg atccgcatca
    12301 ccgattacaa gcggaatctt gatcaaaatg ccaaatttca cgcgatggtc gcggatatct
    12361 cccgacaggt tcagtggtat ggcaggtggc taaaaccaga acagtggaaa gttttgttga
    12421 tcagcggtca tgccgtggcg acaaaacagg aagctgatgt tttgccaggt cttgaaggtg
    12481 aatacgtcaa tatccgcgaa agcagtgcgc agatgagcgt gaagcgtatg gcaagcctga
    12541 ttgagtacac aacttcctgg gctgtggagc aggggatcag atttaccgac aggaggtacg
    12601 aatgagacga aagcgacgaa gcatcaccga tatagtctgt gaaaactgca attaccttcc
    12661 aacgaaacgc tcccgaaata aacccaggcc aatccccaac gaatctgacg ttaaaacctt
    12721 caattacacg gctcacctgt gggatgtccg ttggctcaga cgtcgcgcaa gatttaatag
    12781 tcattccgga tagttcaatg tacgaggaat aagatgatgg caaacctacg caaagaagcg
    12841 cgtggcagag aatgtcaggt acggatttac ggcgtatgca atggcaatcc tgaaactaca
    12901 gttttggcgc attaccgaat ggctggaatt tgcggaacgg gaatgaagcc agatgacctg
    12961 ttggctgcat gggcctgtag tgactgccat aatgaaatcg atcgccgtac tcgcattctc
    13021 gacaacaacg acgccagact ttaccacctg gaaggcgtga tcaggacgca ggcgatactg
    13081 ctgaaggagg ggaaaattaa gtcatgaatg aatatgagtt tgtgcttccc tggccgccga
    13141 cggtgaatac ctactggcga agacggggaa gccagtacta catcagcgat aaaggccaga
    13201 aataccgaaa agacgtacag caaatcatcc ggcaactcag attagacatt ttcactaaat
    13261 cacgacttcg catcacaatt attgctgaac caccagattc ccgccgtcgc gacctcgata
    13321 acatcctgaa aggtttactc gactctctta tccacgccgg atttgcggaa gacgacgagc
    13381 aattcgatga cattcgcgta attcgcggcg tgaaagtgcc tggcggtaga gtggggataa
    13441 aaatcaccga actggagaac atttgatgaa tgctaaaatt caaacgatac ctgaattact
    13501 gatctgcacc aggggaaatc agacagaagt cgccagaata ctgaactgca atcgtgccac
    13561 agtcagaaaa tacattgatg ataaagatgc gaaaaagcac gccgtcgtca atggcgtcct
    13621 tatggttcat cgcggatggg gtaaagatac tgatgcgtga tatccggcag gttcttgagc
    13681 gctggggtgc atgggcggca aataactatg aggatgttac atggtcgccc attgctgccg
    13741 gatttaagga actgatcccc gaaaaagtaa aatcacgtcc acagtgttgt gacgatgacg
    13801 cgatgattat atgcgggtgt atggctcgcc ttaacaggaa caacagcgat ctgcatgact
    13861 tgctggttga ttactacgtg ttgggggaga cgttcatggc gctggcacgt aagcatggat
    13921 gctctgatgg aactatcggt aaaaaattac agaaagcgga gggcgtggtt gaagggatgt
    13981 taatgttact tggtgtcaga ctggaaatgg accgatatgt gcaacgcctc tgacaggagg
    14041 cgtttgccgt tcactatgtg aagtgtgtca atttgtgagt gttatgctat ttactttatg
    14101 ggagcggaac gtaagagtta tccacctgtt aactgcggag atgcaacaat gaacaggggg
    14161 caaaatggac atcgaagatt tcctggaaga agaaaacgat ttggctctgt ggaatgatga
    14221 tattttgagt attgactggt tcatcatcct taaagaactt ttgaaatacc agcacaggtt
    14281 aaagttaaac cattcagaat tacttctgct ggctaatttt gtttcatttc accaggatgc
    14341 agattcacaa acattaccat caatatctct ttttgccacc agaatgcgta catcccggga
    14401 cactatacaa ggagtcctgg tgcgtctgga ggagaaagat atgttacaga agataagtta
    14461 cgcgcaaatc gatcatggcg atgatttgcg aaacatctat gatatcaagc cattaatccg
    14521 ccggttaatt tggttattga aaagtggttg tgataaaaag catacttgcc ccctctgcgg
    14581 gaaagtagct atatctaatg aggaaattga gaaaaaattc ggattcagaa tttgtgggaa
    14641 taagaaaagg ccgcaaagct ggtgtcgttc atgcagaagc cccaaacaac gccgactgaa
    14701 tacattgcta tgtggtaaaa aaagagctcg aaaatcctcg cccgaggtgg tgtaaagcgt
    14761 catagacccc cggtcctctt gtttgattcc acacctgaat aaagtgggat gccaaaaaag
    14821 tattttacgt acgtaaaaat ctgcatatca tgataagagt ggttacattt ccacgcagcc
    14881 gaacccgccg atgcgcgggt tttttgtatc cggaatcctg t
//
LOCUS       DAZUCC010000124        14917 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZUCC010000124 DAZUCC010000000
VERSION     DAZUCC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.125"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1441
                     /gene="ygcU"
                     /locus_tag="ACHY09_003214"
     CDS             <1..1441
                     /gene="ygcU"
                     /locus_tag="ACHY09_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFS3886151.1"
                     /translation="AAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHGIYTLP
                     IPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDGSAMNQ
                     IINIDIENMQVTAQCGVPLEVLENTLREKGYTTGHSPQSKPLAQMGGLVATRSIGQFS
                     TLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITEVTVKI
                     FKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKC
                     VLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNHLNWGPDKVAAERVQ
                     ILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNM
                     YFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHG
                     SAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            1534..2871
                     /gene="ygcS"
                     /locus_tag="ACHY09_003215"
     CDS             1534..2871
                     /gene="ygcS"
                     /locus_tag="ACHY09_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3886152.1"
                     /translation="MNTSPVRMDDLPLNRFHCRIAALTFGAHLTDGYVLGVIGYAIIQ
                     LTPAMQLTPFMAGMIGGSALLGLFIGSLVLGWISDHIGRQKIFTFSFLLITLASFLQF
                     FATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFSPRRHRGVLLGAFSVVWTVGYVLAS
                     IAGHHFISESPEAWRWLLASAALPALLITLLRWGTPESPRWLLRQGRFAEAHAIVHRY
                     FGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFNSVFFVCLVIPWFVIYTWLPTIAQT
                     IGLEDALTASLMLNALLIVGALLGLVLTHLLAHRRFLLGSFLLLAATLVVMACLPSGS
                     SLTLLLFVLFSTTISAVSNLVGILPAESFPTDIRSLGVGFATAMSRLGAAVSTGLLPW
                     VLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKALPLVAAGNVGGANEHSVSV"
     gene            2849..3628
                     /gene="ygcR"
                     /locus_tag="ACHY09_003216"
     CDS             2849..3628
                     /gene="ygcR"
                     /locus_tag="ACHY09_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFS3886153.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNIGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEDAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEITRETVAAEAPA
                     IQCLQLARPEQRRRAALIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3625..4485
                     /gene="ygcQ"
                     /locus_tag="ACHY09_003217"
     CDS             3625..4485
                     /gene="ygcQ"
                     /locus_tag="ACHY09_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFS3886154.1"
                     /translation="MNIAIVTINQEHAAMAGWLAAQDFSGSTLTHWQIEPQPMVAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELSIRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAEINTMLPSGMLQLNIVPGALPDWLISIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLVEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(4632..5207)
                     /gene="ygcP"
                     /locus_tag="ACHY09_003218"
     CDS             complement(4632..5207)
                     /gene="ygcP"
                     /locus_tag="ACHY09_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFS3886155.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(5224..5484)
                     /gene="ygcO"
                     /locus_tag="ACHY09_003219"
     CDS             complement(5224..5484)
                     /gene="ygcO"
                     /locus_tag="ACHY09_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFS3886156.1"
                     /translation="MSVTRNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDEKTLQQWRYPPSGFGITYRFG"
     gene            complement(5475..6746)
                     /gene="ygcN"
                     /locus_tag="ACHY09_003220"
     CDS             complement(5475..6746)
                     /gene="ygcN"
                     /locus_tag="ACHY09_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS3886157.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGTQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6824..7186)
                     /gene="queD"
                     /locus_tag="ACHY09_003221"
     CDS             complement(6824..7186)
                     /gene="queD"
                     /locus_tag="ACHY09_003221"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFS3886158.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHYYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            7505..9304
                     /gene="cysJ"
                     /locus_tag="ACHY09_003222"
     CDS             7505..9304
                     /gene="cysJ"
                     /locus_tag="ACHY09_003222"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFS3886159.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVDGKTLTLSEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEDGEVRVFIEHNDNFRLPAN
                     PETSVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9304..11016
                     /gene="cysI"
                     /locus_tag="ACHY09_003223"
     CDS             9304..11016
                     /gene="cysI"
                     /locus_tag="ACHY09_003223"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFS3886160.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            11090..11824
                     /gene="cysH"
                     /locus_tag="ACHY09_003224"
     CDS             11090..11824
                     /gene="cysH"
                     /locus_tag="ACHY09_003224"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFS3886161.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            12183..14849
                     /gene="cas3"
                     /locus_tag="ACHY09_003225"
     CDS             12183..14849
                     /gene="cas3"
                     /locus_tag="ACHY09_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFS3886162.1"
                     /translation="MEPFKYICHYWGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQ
                     SVVLQNAFCRNEMLSKQKVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLN
                     GPSTQMCRKFNHGAAGLYWFNQDSLSEQSPGDFFSFFDAAPHPYESWFPWVEAVTGHH
                     GFILHSQDQDKSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCS
                     SLLAGFCSLADWLGSWTTTNTFLFKEDAPSNINALRTYFQDRQQDASRVLELSGLVSN
                     KRCYEGVHALLDNGYQPRQLQVLVDALPVAPGLTVIEAPTGSGKTETALAYAWKLIDQ
                     QLADSVIFALPTQATANAMLSRMEANASRLFTSPNLILAHGNSRFNHLFQSIKSRAFT
                     EQGQEEAWVQCCQWLSQSNKKVFLGQIGVCTIDQVLISVLPVKHRFIRGLGIGRSVLI
                     VDEVHAYDTYMNGLLEAVLKAQADVGGSVILLSATLPMKQKQKLLDTYGLHTDPEENN
                     SAYPLINWRGVNGAQRFDLLAHSEQLPPRFSIRPEPIYLADMLPDLRMLERMIVAANA
                     GAQVCLICNLVDVAQLCHQRLKELNNTQVDIDLFHARFTLNDRREKENRVISNFGKNG
                     KRNVGRILVATQVVEQSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEVPL
                     ATVLLPDGEGYGRHEHIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEM
                     DEPEWVIKGMDKFESAECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLP
                     YLQTSSGKQLLDGQVYEDLSHEQQYEALALNRVNVPFTWKRNFSEVVDEDGLLWLEGK
                     QNQDGWIWQGNNIVITYTRDEGMTRVIPANPK"
BASE COUNT         3681 a         3695 c         4010 g         3531 t
ORIGIN      
        1 cgcagcgatt gtcgaccagc taaaggaaat tgttggtgca gatcgcgtaa ttaccgatga
       61 aacagtatta aagaaaaaca gtattgaccg ttttcgcaaa tttccggata ttcatggcat
      121 ttatactttg ccgattccgg cagcggtcgt aaaactcggt tccacagaac aagtctctcg
      181 tgtactgaat tttatgaatg cacacaaaat taacggtgtg ccgcgtaccg gtgcttccgc
      241 taccgagggt ggactggaaa ccgttgtgga aaactcggtg gtgctcgacg gctctgccat
      301 gaatcaaatc attaatattg atattgagaa tatgcaggtc acggcgcaat gtggtgttcc
      361 gctggaggtg ctggaaaaca cgttgcgtga aaaaggttac accacggggc attcaccgca
      421 atcaaaaccg ctggcgcaga tgggcggcct ggtagcaacc cgcagtatcg ggcagttctc
      481 cacactctac ggcgcaatcg aagatatggt cgttggtctg gaagcagtat tggctgatgg
      541 caccgtcaca cgtattaaaa acgtaccacg ccgcgcggca ggcccggaca ttcgtcacat
      601 tatcatcggc aacgaaggtg cattgtgcta tatcactgaa gtcacagtga aaatcttcaa
      661 atttacgccg gaaaacaatc tcttctatgg ctatgtgctg gaagacatga aaaccggctt
      721 caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc gtttgtatga
      781 cgctgaagat ggcacccaac acttcaccca ttttgccgat ggtaaatgtg tgctgatctt
      841 tatggctgaa ggtaaccctc gcattgcgaa ggcgacaggc gaagggattg ctgaaatcgt
      901 ggcacgttac ccgcaatgcc agcgagtgga cagcaagctg atcgaaacct ggtttaatca
      961 cctgaactgg gggccggata aagtggccgc tgaacgtgtg cagatcctca aaaccggcaa
     1021 catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa tctacgaaag
     1081 cgttattaac cgtattcgta ccgagttccc gcatgccgac gacatcacca tgctgggcgg
     1141 tcactcctct catagctatc agaacggcac caacatgtac ttcgtctacg actacaacgt
     1201 cgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca acaaaatcat
     1261 ctgcgaagaa accattcgcc tcggtggttc gatggtgcac caccacggta tcggtaaaca
     1321 tcgcgttcac tggagcaaac tggaacacgg cagcgcgtgg gcgttgctgg aagggctgaa
     1381 aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga ttgaaaaata
     1441 atgtgtcagg cagcgttccg cgatgacggg cgttgccttt tcggcttctc aggcgagaag
     1501 ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt gcgaatggat
     1561 gatttaccac ttaaccgttt tcactgccgc attgctgcgc tcactttcgg cgcacacctg
     1621 accgacggtt atgtcctcgg cgtcattggt tacgccatta ttcagcttac acccgccatg
     1681 caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctacttgg cctgtttatc
     1741 ggtagcctgg ttttggggtg gatctccgac catattggtc ggcaaaaaat cttcaccttc
     1801 agctttttgc tgattacgct cgcttcgttc ttacaatttt ttgccaccac gccagagcat
     1861 cttattggac tgcgcatttt gatcggcatt ggtctgggag gcgattactc agtcggtcac
     1921 actttgctgg ctgaattttc tccgcgccgc catcgcggtg ttttgctggg cgcattcagc
     1981 gtggtgtgga ccgtaggcta tgtgctggca agtattgccg gacatcactt tattagcgaa
     2041 agcccggagg cctggcgctg gctactggca tcggcagctc tgcccgcatt gttgattacg
     2101 ttattacgct ggggaacgcc agaatcgcca cgctggctac tgcgccaggg gcgttttgca
     2161 gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg cgatgaagtg
     2221 gcaacggcga cccataaaca catcaaaacc ttgttctctt cgcgctactg gcggcgcaca
     2281 gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat ttatacctgg
     2341 ctgccgacca tcgcccaaac tattggtctg gaagatgcgc tgactgccag cctgatgctt
     2401 aatgcgttgt taattgttgg cgcgttatta gggttagtgt tgacccactt gttagcgcat
     2461 cgcagatttt tactgggaag ttttttgctg ctggcggcaa cgctggtcgt gatggcctgt
     2521 ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac caccatttcg
     2581 gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga cattcgctcg
     2641 ctgggcgtcg gttttgccac cgccatgagt cggttggggg cggcggtaag tactggcctg
     2701 ctgccgtggg tgctggcgca gtggggaatg caagccacct tattgctcct ggcggcagtg
     2761 ttgttggttg gttttgttgt gacctggcta tgggcaccag aaaccaaagc actcccgctg
     2821 gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta aagccgaacc
     2881 ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta atatcggacc
     2941 ggatgtttcg cttctgcgaa gtttactcgg tgccgatgaa caggctgccg ccgcgctgct
     3001 gttggcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca tgggggatga
     3061 acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggacg ctgtattgct
     3121 ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata ttgccgaatg
     3181 gcagcgtcag aatccgctgg atctgatcat taccggctgc caaagcagcg aagggcagaa
     3241 tggacaaacg ccatttttgc tggcggaaat gctgggctgg ccctgtttca cccaggtgga
     3301 gcgttttact ctcgacgcgc cgtttatcac cctcgaacag cgtactgaac atgggttgcg
     3361 ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgtggtg aagtggcctt
     3421 gccagtaccg ggaatgcgcc agcgaatggc agcggggaaa gcggagatta cccgcgaaac
     3481 tgttgccgcg gaagcgccag cgatacagtg cctgcaactg gcaaggccag agcaacgacg
     3541 cagagcggcg ctgattgatg ggcaaacggt agcagaaaaa gcgcaaaagc tatggcagga
     3601 ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa tcaggaacat
     3661 gccgcaatgg cgggctggct ggcggcgcag gatttcagcg gcagcacact aacgcattgg
     3721 caaatagaac cacagccgat ggttgcggaa caggtgctgg atgcgctggt ggaacagtgg
     3781 caacgaacgc ctgcggaggt tgtccttttt cctccgggcg cgtttggcga tgaactgtcg
     3841 atccggctgg cctggcgttt acatggtgca agcatctgcc aggtgacttc gttagatatt
     3901 ccaaccgtaa gcgtgcgcaa atcccactgg ggaaatgcgc taacggcgac gctgcaaaca
     3961 gaaaaacgcc cgctatgtct ttccctggcg cgtcaggcgg gagcagagat aaacaccatg
     4021 ttgccctctg gtatgctgca actgaacatt gtccccggtg cgcttcccga ctggctgatt
     4081 agcatagaaa acctgaaaaa tgttacccgc gatccattag ctgaagccag acgggtgctg
     4141 gtggtggggc agggcggaga ggcagataac caggaaatcg ctatgctggt agaaaagctg
     4201 ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga tgctgaaaaa
     4261 gtgattggta tttccggaca tctgctggcg cctgaggtgt gcattgttgt tggcgcttcc
     4321 ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttgtggtggc gattaatcat
     4381 gacgccagcg cggcggtatt ttcacaggca gatgttggcg tggttgatga ctggaaagcg
     4441 gtgctggagg cactggtgac aaccattcac gcagattgcc aataacgtgc tgccggatga
     4501 tcctgcctac ggttctgggc tggggacttg taagcctgat aagacgcgtc aagcgtcgta
     4561 tcaggcgctg aatgtcggat gtggcgtgca gcctacaaat cacacaaatt caactcgtta
     4621 cgagtatccc gtcaaagcaa ttttttcgct aacgtccaga ccccggtatt cgtggtggaa
     4681 agcgccacaa cacccgcgtt aatcgcatta cgcgcatctt cttcatcgca caccagcccg
     4741 ccggcaatca gcggttggcg gattttctcc gtcacccagc ccagcacttt aggcatacag
     4801 cctggcagga tctcaataca atccggattc gattgcgcaa cttgcttatc aatgttgtga
     4861 aacgaaatcg aatcaacaat aaacaggcga tgaatacaga aaaaaccttc cgctcttgcc
     4921 gctttcaaca ttgaggcttt ggtactgata atgccgtccg cctcagtcac cagtttcaaa
     4981 aactgaatca ccacttcttt atttgacgcg ccttccagca aatcaacatg aataaaagca
     5041 tattttccgg catttttaat ctttttaacg atttgactaa tggtgcagat attgccgtac
     5101 aacacggaaa taaactggca ttcagagtcg attgccagtt gcaggctggc attatcttta
     5161 acggcagcaa tcaccggatt ctggcggagc aggtgtaaga ggggcataaa ttatccttat
     5221 tatttatcca aaacggtagg tgatgccgaa tccggaaggc ggatagcgcc actgttgtag
     5281 tgttttttca tcgcacagca aacggcaggt gccacactcc aggcagccgc gataatcaac
     5341 gcgtaagtca ccttccggtg tgagcgaaaa aagaccagcc ggacaagcgt taatcaaccg
     5401 ttgtaccgtc tgacgctcaa cggagtcagc cggtacaatg tgcggcgcat cggcaacgcg
     5461 ccagagatta cgggttacag acatcgcaga ctcctgataa catcgcccgc cagatgccac
     5521 aggccgtgac gacgtaaatg acgccagaat aactggcgca gcggtggaac aggtttatca
     5581 ccctgatccc ataaatcgcg ggaaatatcc tgcattaacg caggccacgc acggtaccat
     5641 ccagggcgtt gcaaaagcgc cggaacatgc tgataacgtt gtagaacatc ccatagcagg
     5701 ctgcgctcta cgttgtgatg ataaagcgca aacagatttt gcggctcgcg gtgctggcag
     5761 gcgcttatca gcgtttgtgc cgccgcctgc gtgccagtca gcgccatatc catgccgcgc
     5821 acggaaattc cggtattgac gcaactgcgc aacgcatcgc ccagcagcag ccagccgtta
     5881 ccggcgtatt gcaccggcat actgtgcaag ccaccttctg gcaccagatg cgcaccatac
     5941 tccagtgatt ccgtgttttt gataagcggg cgcactgccg gatgcgtttt aaagcgagcc
     6001 agcagctcgc ttgccggaac acgactttgc gtaagggaag agagcgggca aacaatccct
     6061 aacgagagcg tttgttgatt agtataaaga aatgcgccgc cgggtaagtc atcacagatc
     6121 ccgccgctga acagcaacgc tgcgccttcg ttattctcca gatgaaaacg ttcttcaata
     6181 gcggatgttt ccagcgacag cacttctttg atccccaacg ccatcgcttc gccagcagga
     6241 cgagtcacta acccgtgacg ttcagccagg acgctgttgg caccttctgc cagcaccaca
     6301 taacgggcgc ggagaatatc gtcaccacaa atgacaccac acaccctgcc gttttcttca
     6361 tacagcgcat ccaccgtcgc accggggatg cattcgacac cttctttttc ggcttcggca
     6421 accagccacg gatcgaaccg tgcacgtaat acactccagg attcaccgcc gggctgtaag
     6481 ctggaaaacg tcgttgcgcc atccggcgtt aacagggaaa ggctttcgtg agtgatgcgt
     6541 cgttcaagag gcgcggtcag gtgaaactga gggaggagtt ccgcgagtgc atgggtatat
     6601 aaccgcccgc cggaaagatt tttgctgccg gggatttcag cgcgttccag taacaaaacg
     6661 gataaacccg ctcgcgcgca gcgtaacgcg caagcggtgc ctgcaatacc ggcaccaata
     6721 ataataatgt cgcagtcgtc ttccatgtca tgccccagat atccagaaca caacacccta
     6781 acatagcgtt acttaaggga aattgaccgc cgacacactc ttatcattcg ccgcgataga
     6841 tgcaacctgc ggtgcaggtt tcttttacca tcaccgcact taacagcggc acaacgggtt
     6901 taacttgatc ccaaatccat tttgctaaaa cctcgctggt cgggttttcc agacctggaa
     6961 tatcattgag ataatagtga tcgaggcgct cgtaggttgg cttaaacgcc gctttcagtt
     7021 cagcgaaatc gataatccag cccgtatgcg gatcgacttc cccggtaatt tccagtcgca
     7081 ccataaagga atgcccgtgc aggcgaccac atttatgccc ttccgggacg tgtggtaagc
     7141 ggtgagcggc ttcgaaggtg aaatctttaa ataacgtggt ggacatcata atttctctac
     7201 aggacgataa aaccgccgta gagtaccgga aaggctgttt tttcgcatta tctaaaacga
     7261 ctttgttgcg caaaatcgct gatttatctt aatgattggc taaattcatt tgtttttcat
     7321 tgggttggtt aatctgtttt gttgttaaag actattgcta aaacaggtta gtcgatttgg
     7381 ttattagtta tcgctatccc gtctttaatc cacaccgttc accccgttaa ccttacctcc
     7441 tcttctgttt tatgggcgct gacagggcgc agaaacagct ttgcttactg gaacataacg
     7501 acgcatgacg acacaggtcc caccttccgc gttgcttccg ttgaacccgg agcaactggc
     7561 acgccttcag gcggccacga ccgatttaac tcctacccag cttgcctggg tttctggcta
     7621 tttctggggc gtactcaatc agcagcctgc tgcgcttgca gcgacgccag cgccagccgc
     7681 agaaatgccg ggtataacta ttatctccgc ctcgcaaacc ggcaatgcgc gccgggttgc
     7741 tgaagcatta cgcgatgatt tattagcagc aaaactgaac gttaagctgg tgaacgcggg
     7801 cgactataaa ttcaaacaaa tcgccagcga aaaactgctc atcgtagtga cgtcaacgca
     7861 aggggaaggg gagccgccgg aagaagccgt cgcgctgcat aagttcctgt tctccaaaaa
     7921 agcgccgaag ctggaaaata ccgcttttgc cgtgtttagc ctcggcgata gttcttatga
     7981 atttttctgc cagtccggga aagattttga cagtaagctg gcggaactgg gcggtgaacg
     8041 cctgctcgac cgtgtcgatg ccgacgttga ataccaggct gctgccatcg agtggcgcgc
     8101 ccgcgtggtt gatgcgctta aatcgcgtgc gcctgtcgcg gcaccttcgc aatccgtcgc
     8161 tactggcgcg gtaaatgaaa tccacaccag cccgtacagc aaagacgcgc cgctggtggc
     8221 gagcctctct gttaaccaga aaattaccgg gcgtaactct gaaaaagacg ttcgccatat
     8281 cgaaattgac ttaggtgact ctggcctgcg ttaccagccg ggtgacgcgc tgggcgtctg
     8341 gtatcagaac gatccggcac tggtgaaaga acttgtcgaa ctgctgtggc tgaaaggcga
     8401 tgaacctgtc accgttgacg gcaaaacgct gaccctgagc gaagcgctac agtggcactt
     8461 tgaactgacc gtcaacaccg cgaatattgt tgagaactac gccacgctta cccgcagcga
     8521 aacgctgctg ccgctggtgg gcgataaagc gaagttacag cattacgccg cgaccacgcc
     8581 gattgttgac atggtgcgtt tctctccggc gcaactggat gccgaagcgc tgattaacct
     8641 gctgcgcccg ctgaccccgc gcctgtattc catcgcctcc tcgcaggcgg aagtcgagaa
     8701 cgaagtgcac gttaccgttg gtgtggtgcg ttacgacgtg gaaggccgcg cccgtgccgg
     8761 tggtgcctcc agcttcctcg cagatcgtgt ggaagaagat ggtgaagtcc gcgtctttat
     8821 cgaacataac gacaacttcc gcctgccagc caatccggaa acgtcggtga ttatgattgg
     8881 cccaggcacc ggcatcgcgc cgttccgcgc ctttatgcag caacgcgccg ccgacgaggc
     8941 gccggggaaa aactggctgt tctttggcaa cccgcacttt acggaagatt tcctctacca
     9001 ggtggaatgg cagcgctacg tcaaagaggg cgtgctgacg cgtatcgatc ttgcctggtc
     9061 gcgcgatcaa aaagaaaaaa tttacgtaca agacaaactg cgcgaacagg gcgcggagct
     9121 gtggcgctgg atcaatgacg gtgcccacat ttatgtctgc ggcgacgcta atcgcatggc
     9181 gaaagacgtt gagcaggcac ttctggaagt gattgccgaa tttggtggca tggacaccga
     9241 agcggcggat gaatttttaa gtgagctgcg cgtagagcgc cgttatcagc gagatgtcta
     9301 ctaatgagcg aaaaacatcc agggccttta gtggtcgaag gaaaactgac agacgccgag
     9361 cgcatgaagc ttgaaagcaa ctacctgcgc ggcaccattg cggaagattt aaacgacggt
     9421 ctgaccggcg gctttaaggg cgacaacttc ctgctgatcc gcttccacgg catgtatcag
     9481 caggatgacc gcgacatccg cgccgaacgt gctgaacaga agctggagcc gcgccacgcg
     9541 atgctgttgc gctgccgtct gccgggtggc gtgatcacca ccaaacagtg gcaggcgata
     9601 gacaaatttg ccggtgaaaa caccatctat ggcagcattc gcctgaccaa ccgccagacg
     9661 tttcagttcc acggcattct gaaaaagaac gtcaaaccgg tgcaccagat gctgcactcg
     9721 gtcggccttg atgcgctggc gactgctaac gacatgaacc gtaacgtact ctgcacctcg
     9781 aacccttacg agtcgcaact gcacgcggaa gcatatgaat gggcgaagaa aatctctgaa
     9841 catctgctgc ctcgtacccg cgcgtatgcg gagatctggc tcgatcagga aaaagtcgcc
     9901 actactgatg aagagccgat ccttggtcag acctacctgc cgcgtaagtt caaaaccacg
     9961 gtagtgatcc cgccgcagaa cgatatcgat ctgcacgcca acgacatgaa cttcgtggca
    10021 atcgccgaaa acggcaagct ggtgggcttt aacctgttgg tgggcggtgg gctttccatc
    10081 gaacacggca acaagaaaac ctacgcccgc accgcgagtg agtttggcta tctgccgctg
    10141 gagcatacgt tggcagtggc ggaagccgtc gtgacaactc agcgtgactg gggtaaccga
    10201 accgatcgta aaaatgccaa aaccaaatac acgctggagc gcgtgggggt tgagacgttt
    10261 aaagcggaag tggagcgtcg cgcggggatc aaatttgaac cgatccgtcc gtatgagttt
    10321 accggacgcg gcgatcgtat tggctgggtt aagggcattg atgataactg gcatctgacg
    10381 ctgtttatcg aaaatggtcg catccttgat tatcctgggc gtccgctgaa aaccggcctg
    10441 ctggagatcg cgaagatcca caaaggcgat ttccgcatta cggcgaacca gaatctgatc
    10501 atcgccggtg tgccggaaag cgagaaagcg aagatcgaga agatcgccaa agagagcggg
    10561 ttaatgaatg ccgtcacgcc gcagcgtgaa aactcaatgg cctgcgtgtc attcccgact
    10621 tgcccgctgg cgatggccga agccgagcgt ttcctgccgt cttttatcga caacatcgat
    10681 aatttaatgg cgaaacatgg tgtcagcgat gagcatatcg tgatgcgtgt aacaggctgc
    10741 ccgaacggtt gtggtcgcgc gatgctggcg gaagtgggcc tggtgggtaa agcgccaggt
    10801 cgctacaact tgcatcttgg cggcaaccgc attgggacac gtatcccacg gatgtataaa
    10861 gaaaacatca ccgagccgga aatcctggcg tcgcttgatg aactgatagg gcgctgggcg
    10921 aaagagcgcg aagcgggtga aggcttcggc gactttacgg tgcgtgcggg catcattcgc
    10981 ccggtgctcg atccggcgcg cgatttgtgg gattaaccat tagcccggtc ttgtaggcct
    11041 gataagacgc gtgagcgtcg catcaggcaa ggcaaacagt gaggaatcta tgtccaaact
    11101 cgatctaaac gccctgaacg aactgccgaa ggtagatcgc attctggcgc tggcggaaac
    11161 taacgccgaa ctggaaaaac tggacgctga aggccgcgtt gcctgggcgc tggataatct
    11221 gcccggtgaa tatgtacttt cttccagctt tggcattcag gcggcggtga gcctgcatct
    11281 ggtgaatcag attcgcccgg atattccggt gatcctcacc gataccggct atctgttccc
    11341 agaaacctac cgctttattg acgagttaac ggacaaactc aagctcaacc tgaaagtgta
    11401 ccgcgctacc gaaagcgccg cctggcagga agcgcgctac ggtaaactgt gggagcaggg
    11461 tgttgagggg attgagaagt acaacgagat caacaaagtc gaaccgatga accgggcgct
    11521 gaaagaactg aatgcgcaaa cctggtttgc tggcctgcgc cgtgaacaat ccggcagccg
    11581 cgccaattta ccggtgctgg caattcagcg tggcgtattt aaagtgctgc cgattatcga
    11641 ctgggataac cgaactattt atcagtacct gcaaaaacat ggcctgaaat atcacccatt
    11701 atgggacgaa ggatatttat cggtcggtga tacccataca acccgtaaat gggaacccgg
    11761 catggcggaa gaagaaacgc gtttctttgg cttaaaaagg gaatgtgggt tacacgaagg
    11821 gtaaatattg ccggataaat taaacgcctc tgtcagaaat gatgggggcg tttttatttt
    11881 gcgagcagca ttccggcatt taaaggtgaa catcgcaggg agaattaaca ggtatagaat
    11941 ggagttgggt gcttgaggct gtctggcgtt aagcattcgc gaggttccag atggacaaaa
    12001 gccccaggcg atatttctat caacctgagg ccagcgttcg aacctaagca attcgaatgt
    12061 tagtctcttc tttgcaggga ggcaagacat gtgtatatca ctgtaattcg atatttatga
    12121 gcagcatcga aaaatagtcc actgatatca tcgataatac taaaaaaaca gggaggctat
    12181 taatggaacc ttttaaatat atatgtcatt actgggggaa atcctcaaaa agcctgacga
    12241 aaggaaatga tattcatctg ttgatttatc attgcctgga tgttgcagct gttgcagatt
    12301 gctggtggga tcaatcagtc gtactacaaa atgctttttg ccgaaatgaa atgctatcaa
    12361 aacagaaggt gaaagcctgg ctgttatttt tcattgccct tcatgatatc ggaaagtttg
    12421 atatacgatt ccaatataaa tcagcagaaa gttggctgaa attaaatcct gcaacgccat
    12481 cacttaatgg tccatcaaca caaatgtgcc gtaaatttaa tcatggtgca gccggtctgt
    12541 attggtttaa ccaggattca ctttcagagc aatctcccgg ggattttttc agtttttttg
    12601 atgccgctcc tcatccttat gagtcctggt ttccatgggt agaggctgtt acaggacatc
    12661 atggttttat attacattcc caggatcaag ataagtcgcg ttgggaaatg ccagcttctc
    12721 tggcatctta tgctgcgcaa gataaacagg ctcgtgagga gtggatatct gtactcgaag
    12781 cattattttt aacgccagcc gggttatcta taaacgatat accacctgat tgttcatcac
    12841 tgttagcagg tttttgctcg cttgctgact ggttaggctc ctggactaca acgaatacct
    12901 ttctgtttaa agaggatgca ccttccaaca taaatgctct aagaacgtat ttccaggacc
    12961 gacagcagga tgcgagccgg gtattggagt tgagcggcct tgtatcaaat aagcgatgtt
    13021 atgaaggtgt tcatgctcta ctggacaatg gctatcaacc cagacaatta caagtgttag
    13081 ttgatgctct tccagtagcg cccgggctga cggtaataga ggcacctacg ggttcaggta
    13141 aaactgaaac tgcgctggcc tatgcctgga aacttattga ccaacaactt gcggatagtg
    13201 ttatttttgc cctccctaca caagctactg caaatgcaat gctcagcaga atggaagcga
    13261 acgcgagccg tttatttacc tccccaaatc ttattcttgc tcatggtaat tcacgtttta
    13321 accacctttt tcaatcaata aaatcacgcg catttactga gcaggggcaa gaagaagcat
    13381 gggttcagtg ttgtcagtgg ttgtcacaaa gcaataagaa agtgtttctt gggcaaatcg
    13441 gcgtttgcac gattgatcag gtgttgatat cggtattgcc agttaaacac cgctttatcc
    13501 gtggtttggg aattggtcga agtgttttaa ttgttgatga agttcatgct tacgacacgt
    13561 atatgaacgg cttgctggag gctgtgctca aggctcaggc tgatgtgggg gggagtgtta
    13621 ttcttctttc cgcaactcta ccaatgaaac aaaaacagaa acttctggat acttatggtc
    13681 tgcatacaga tccagaggaa aataactccg catatccact cattaactgg cgaggtgtga
    13741 atggtgcaca acgttttgat ctgttagctc attcagaaca actcccgccc cgcttttcga
    13801 ttcggccaga acctatttat ttagctgaca tgttacctga ccttaggatg ttagagcgaa
    13861 tgatcgtagc ggcaaatgcg ggtgcacaag tctgtcttat ctgcaatttg gttgatgttg
    13921 cacagctatg ccaccaacgg ttaaaggaac taaataacac gcaagtagat atagatttgt
    13981 ttcatgcgcg ctttacgctg aacgatcgtc gtgaaaaaga gaatcgagtt attagcaatt
    14041 tcggcaaaaa tgggaagcga aatgttggac ggatacttgt cgccacccag gtcgtggaac
    14101 aatcactcga cgttgatttt gattggttaa ttactcagca ttgccccgca gatttacttt
    14161 tccaacgatt gggccgttta catcgccatc atcgcaaata tcgtcccgct ggttttgagg
    14221 ttcctcttgc caccgttttg ctgcctgatg gcgaaggtta cggacgacat gagcatattt
    14281 atagcaacgt tagagtcatg tggcggacgc agcaacatat tgaggaactt aatggagcat
    14341 ccttattttt ccccgatgcc taccggcaat ggctggatag catttacgat gatgctgaaa
    14401 tggatgagcc agaatgggtc atcaaaggca tggataagtt tgaaagcgct gagtgtgaaa
    14461 agaggttcaa ggctcgcaag gttctgcagt gggctgaaga atatagcttg caggataacg
    14521 atgaaacaat tcttgcggta acgagggatg gggaaatgag cctgccatta ttgccttatt
    14581 tacaaacgtc ttcaggtaaa caactgctcg atggccaggt ctacgaggac ctaagtcatg
    14641 aacagcagta tgaggcgtta gcacttaatc gcgtcaatgt acccttcacc tggaaacgta
    14701 atttttctga agtagtagat gaagatgggt tactttggct ggaagggaaa cagaatcagg
    14761 atggatggat ctggcagggt aataatattg ttattaccta cactcgggat gaagggatga
    14821 ccagagtcat ccctgcaaat cccaaataac ttcgggaatg attggtatca atgacgataa
    14881 caagaccaat aacggtttat ccctacttaa gtaggga
//
LOCUS       DAZUCC010000125        14731 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZUCC010000125 DAZUCC010000000
VERSION     DAZUCC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.126"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..468
                     /locus_tag="ACHY09_003226"
     CDS             <1..468
                     /locus_tag="ACHY09_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFS3886163.1"
                     /translation="LLEKGGEIAVRFKIPENVNLETTRNKYAFGLYWRIAEWPGEGGE
                     GYLSSFFVQTDKASIDVAYHHTGNQQKELGTFGAFDHDWHTLAFKFKGSNSINVTPVL
                     DGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDTVEVKANKAGAAA"
     gene            608..790
                     /locus_tag="ACHY09_003227"
     CDS             608..790
                     /locus_tag="ACHY09_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFS3886164.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            816..1088
                     /locus_tag="ACHY09_003228"
     CDS             816..1088
                     /locus_tag="ACHY09_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFS3886165.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            1165..1380
                     /locus_tag="ACHY09_003229"
     CDS             1165..1380
                     /locus_tag="ACHY09_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS3886166.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            1385..1918
                     /locus_tag="ACHY09_003230"
     CDS             1385..1918
                     /locus_tag="ACHY09_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS3886167.1"
                     /translation="MSRKFRYGLSAVVLAMIAAGASAPEILDQFLDEKEGNRTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCLPSTFYKRLNAGDRKGACESIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            1915..2382
                     /locus_tag="ACHY09_003231"
     CDS             1915..2382
                     /locus_tag="ACHY09_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS3886168.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPRLADTVERDYFTLRERLMMMQKQLEGAQEYIRTQCLK"
     gene            2685..3491
                     /locus_tag="ACHY09_003232"
     CDS             2685..3491
                     /locus_tag="ACHY09_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFS3886169.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            3472..5178
                     /locus_tag="ACHY09_003233"
     CDS             3472..5178
                     /locus_tag="ACHY09_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFS3886170.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            5178..7322
                     /locus_tag="ACHY09_003234"
     CDS             5178..7322
                     /locus_tag="ACHY09_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS3886171.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            7480..8487
                     /locus_tag="ACHY09_003235"
     CDS             7480..8487
                     /locus_tag="ACHY09_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886172.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            8511..9725
                     /locus_tag="ACHY09_003236"
     CDS             8511..9725
                     /locus_tag="ACHY09_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFS3886173.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            9781..10170
                     /locus_tag="ACHY09_003237"
     CDS             9781..10170
                     /locus_tag="ACHY09_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886174.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            10220..10681
                     /locus_tag="ACHY09_003238"
     CDS             10220..10681
                     /locus_tag="ACHY09_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886175.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQESVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            10665..11228
                     /locus_tag="ACHY09_003239"
     CDS             10665..11228
                     /locus_tag="ACHY09_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886176.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            11228..11878
                     /locus_tag="ACHY09_003240"
     CDS             11228..11878
                     /locus_tag="ACHY09_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFS3886177.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            11875..13911
                     /locus_tag="ACHY09_003241"
     CDS             11875..13911
                     /locus_tag="ACHY09_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFS3886178.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKASEAAEAATRAITAAGNAEESSTRAGESEKAAGADAEKARLHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGDTGERGPKGDQGDMGPKGEKG
                     DPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPGPAGPRGERGETGPQGPRG
                     EPGPAGSAANVADATTAQKGIVQLSSATDSDDETKAATPKAVKAAMDKADGCLEKAKN
                     GDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEFVAWLKEKGAFDQPYWMMKASLL
                     AEFNKVITDVGPGKLNLGGCAIEVMGTYNAAIVRVTIGEYGGDGFLNGTVCTCTVYGD
                     TQRFHWRVDYSTKNKPTTASLTVNGWERDEVTGRLRQWGSIEVSEDDGKLMTQHSIRF
                     PVAFPVAALNAQVSAVGDPHAIKMYTLSNPSLVSVTLITVRGCYGKFSWEAIGY"
     gene            13921..14145
                     /locus_tag="ACHY09_003242"
     CDS             13921..14145
                     /locus_tag="ACHY09_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3886179.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            14145..>14731
                     /locus_tag="ACHY09_003243"
     CDS             14145..>14731
                     /locus_tag="ACHY09_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFS3886180.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKSGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVR"
BASE COUNT         3794 a         3368 c         4342 g         3227 t
ORIGIN      
        1 ttactggaaa aaggcgggga aattgctgtc cggtttaaaa tccccgaaaa cgtcaatctt
       61 gaaacaacca gaaacaagta tgcctttggt ttgtactggc gaatagcgga atggccgggt
      121 gagggtggtg aaggctatct gagttctttc tttgtccaga cagataaagc cagtattgat
      181 gttgcatacc atcatacagg taatcaacaa aaagaacttg gcacgtttgg cgcattcgac
      241 catgactggc atacgcttgc atttaaattt aagggcagta acagcattaa tgttactccg
      301 gtgcttgatg gtgtggatgg acaggcgttt gacctggtga aatgggccaa tactgctaat
      361 ggactcaaca ggtttgtcat tacggatatt acaggtagtg cagaaaccta ccctgtactt
      421 attgatacgg tggaagttaa agcaaacaaa gctggagcag ccgcataatt gctaaaaaaa
      481 gccgccagcg gcaggaatgg aagctggcgg aggtaatccc aatggagaat gtaaagaaaa
      541 gatgctttcg tacattggtt ttttaaatga aaacagttct cattgtcaac cataacggta
      601 agaaattatg atatttattc atcaggtgat gctgtacttc tgtacggcgg tctgtgtgct
      661 gtatcttctt tcgggtgggt acagggctgt gcgcgatttc tggtgcaggc agattgataa
      721 aagggccgct gagaaaatca gcgccagtca gtcagccgga agcaaacccg aagagcccgt
      781 tactccttaa caaccccttt cagcgagaaa atcccatgtc agaaatcaca tccctggtca
      841 ctgcagaggc agtgaaggaa gtcctgcgct ctgaagaagt ccggagcgca ctgaaacaga
      901 aacttcgcca gaatcttgag gcgcgtcttg atgcagaagt tgatgccatt ctggatgagc
      961 tgcttggtgt acaggcagag ccaccgactg aagcgggaga taccaccgca gagagcggtg
     1021 aagttcagcc tgaatcaccg gtcgccgatg cgactgaacc tcaacccgaa tcggtcatga
     1081 tgctgtaacg aggagtcagg gccatcagta aacggctgct ggcctttttc atgttgtgag
     1141 cttccggatt gcgggagacg gggtatgtac cagatggaaa aaatcacaac aggtgtgtca
     1201 tacaccacgt cagcggtggg aacgggctac tggttcctgc agttgctgga cagggtttcc
     1261 ccgtctcagt gggcggcaat aggcgtgctg gggagtctgc tgtttgggct gctgacatat
     1321 ctgacgaacc tgtatttcaa aattaaagaa gaccggcgta aggcggcgcg gggagagtaa
     1381 gctgatgagc aggaaattcc gctatggttt atcagcggtc gttctggcga tgattgctgc
     1441 aggtgcttct gcgcctgaaa tcctcgacca gtttcttgac gaaaaagaag gtaatcgcac
     1501 cacagcatac cgtgatggtg cgggtatctg gaccatctgc cgtggtgcca cccgggtgga
     1561 tggtaagccc gtcgttcccg gcatgaagct gtcgaaggaa aaatgcgcac aggttaatgc
     1621 cattgagcgt gataaggcgc tggcatgggt ggagaaaaac atcaaagtgc cactgaccga
     1681 accccagaaa gcgggtattg cgtcattctg cccgtataac attggccccg gtaagtgtct
     1741 cccgtcgacg ttttataagc ggcttaatgc cggtgatcgt aaaggtgcct gtgagtcgat
     1801 tcgctggtgg attaaggacg gtgggcgtga ttgccgcaca cgttcaaata actgctacgg
     1861 acaggttatt cgtcgtgacc aggaaagcgc attagcttgt tgggggatag atcagtgaga
     1921 agagtaaccg cgattattta cgctctggtt atctgcatca tcgtctgtct gttatgggct
     1981 gttaatcatt accgcgataa cgccatcacc tacaaagcgc agcgcgataa aaaagccaga
     2041 gagctggagc tggcaaacgc aaccattact gacatgcagc agcgccagcg tgatgttgct
     2101 gcgcttgatg ccagatactc aagggaatta gccgatgcga gagctgaaaa tgaaactctg
     2161 cgcgctgatg ttgccgctgg tcgtaagcgc ctgcggatca acgccacctg tccaggctcc
     2221 gtgcgtgaag cccccaccac ctccggcgtg gataatgcaa ccggcccccg actggcagac
     2281 acagttgaac gggattattt caccctcaga gagcggttga tgatgatgca gaagcagctg
     2341 gaaggggcac aggaatatat ccgcactcag tgcctgaaat aagttttgct gatgcgccgt
     2401 atcgtcgccg tattcccgta ttaacagcga ctgcagccct gacgggtacc tcctgcgtga
     2461 gtgtgcgggg ataatcaaaa acgatacaca ccgggtttcc ctcatttttc aggagacgtg
     2521 agcatttacc cgcgaagccg cctgtccggt gcggtggtgg aagaaaccgg ataaaacaac
     2581 ctcattgtac aaatatcgat caagtatggc gctgctgtgt gaattctgaa aaatcacagc
     2641 ggtcattatg cgtcagtttt taacagagga cgtcagaaag tgacatggca aagctggact
     2701 ggaaaaagct ggagcaggca ttccgacgcg aacatgccga aacgggaata acattactgg
     2761 actggtgccg gaagaaaaag attaattaca acaccgccag aacccgtata aaaatgggca
     2821 aaatcgatca tgaaattgat cataaaaccg atcatgaaat cgatcatgac atctcagatg
     2881 aagaacccag caatgacgcg tgttccgacg atgaaaaatg cgcaaaaaac tctgaaaaaa
     2941 actgcgcaaa ttcggcagaa acgaaacgga ttcgtggttc ccgactttta cctccttcaa
     3001 acgctttttc tcagcgaaac acccacgccg taagacaccg tggatatgcg aagtatcttg
     3061 aggcagataa cctcatggat gatgcgtccg acatggtgct gttcgatgaa ctggtgttca
     3121 cccgggcccg cgcactttca gtaactaagg cacttaaagg gatgttcgcc gacctggaag
     3181 aggcaactga cgtggaaacc cgcgttgctc tttacgacaa aatactcaaa gctgaacagg
     3241 cccttgaccg gaatattgcc cgtatcgagt caattgaacg ctcattgctg acgctggacg
     3301 tcctggctga gacagcacca aaacttcgtg ctgaccggga aagaatcaac gccgccagag
     3361 ataaactcag agcggaaacc gatattctga ccagccagcg ccggggggtt attacgccag
     3421 tcagtgacat cgtgtcatcg ctgcatgaaa tgagtaattc ggggagactg gatgacattc
     3481 cggaagaata aaacgcgatg tgatgaacct gcagaaatga ctgagaccga acaacgtctg
     3541 ttcattatga caaaactgag caatccctgg tggcgcctca atcatctcta caaaatacag
     3601 aacgaaaaag gtgaactggt caccttcaga atgcgaccgg cgcaacgtca gttgtttcgt
     3661 aacatgcata ataaaaacat tatcctgaaa gcgcgccagc tgggattttc cacagccatt
     3721 gatatttatc ttctcgacca ggcattattc attccgcatc tcaaatgcgg gatcgtcgct
     3781 caggataaac aggctgccag tgaaattttc cgcacaaaaa ttgctgtacc gtttgatcat
     3841 ctccctgact ggctgagagc ctcattcacc atcgttgaac gtcgtagcgg tgccagcggt
     3901 ggctatatcc tgtttggtca cggctcgagt attcaggtgg caacctcatt tcgctcaggt
     3961 acggtgcagc gcctgcatat ctcagagcac ggcaaaattt gcgcgaaata tccggctaag
     4021 gcgaaagagc tgcgaaccgg tacgcttaat gccgtctctg atgaatgcat tatttttgat
     4081 gaatccacgg ctgaaggcgt gggtggtgat ttttacgaga tgagtaaccg tgcacaggag
     4141 agcactgcat caggcttatt gctgacggca caggattata aattccattt ttacgcatgg
     4201 tggcaggatc ctaaatacag cgccagagtg cctgaaagcg ggctgaagct gtcacgggaa
     4261 aaaatgacgt atttttctgc ggttgagaag gcaatgaaca tcacgcttac cgatgagcag
     4321 aagcagtggt acatcagtaa ggaaactgaa cagcgtgagg aaatgaagca ggagtttccc
     4381 tcaacgccac aggaggcgtt tctgacgtcc ggacgacgtg tgttcagtgc cgaaagtaca
     4441 ttgcaggcag aatcattctg ttcgccaccg atgattgttt atgacattga acctgttaca
     4501 ggagcgaaga ctaaagctca gtctctgcgt gaaggaaata aaaacgagtt gcagcggacg
     4561 ctgatgaatt atctgctggt atgggaactg ccggatccgg atgaagagta tgtttgtggg
     4621 gcagatactg ccgaagggct ggagcacgga gaccgctcat cgctggatgt tgtcaaacgc
     4681 agtaatggcg agcaggtggc tcactggttc gggcatctcg atgctgaact ttttgctcat
     4741 ctcatttcgc aggtctgtcg tatgtataac aacgcgtttg tggggccgga gcgtaataat
     4801 cacggacatg cagttatcct gaaactccgg gaactctatc cgacacgtta tatctacaac
     4861 gaacagcatc ttgaccaggc atatgacgac gatacgcccc gccttggctg gctgacaacc
     4921 cgtcagagca aacctgttct gaccgaagga atgaaaacgc tcctgaataa tggaatatca
     4981 gggatccgct ggtcaggcac attatcggaa atgaacacct acgtttatga cgcgaaaggc
     5041 tccatgaatg cacaggaagg ctgctttgat gatcagctca tgagctacat gattgcccag
     5101 gagatgcgcg ccagaatgcc ggtgagggta aaacagaaaa cggataaacg cagaaccaca
     5161 cactggatgg cacactgatg aaaaatgaaa ttaacaccac agcgatgaaa aacgatcatg
     5221 gatccacgcc gcgtttttct cagcgtcagt tactgtctct ctgttctgat attgacagtc
     5281 agcctctctg gcgtgatgcc gcaaacaagg cctgtgcgta ttatgatggc gaccagctgg
     5341 caccggaagt tatccaggta ctgaaagatc gcggtcagcc catgaccatc cataacctca
     5401 ttgcccccac ggtagatggt gtactgggaa tggaggcaaa aacaagaacg gacctgatag
     5461 tgatgtcaga cgatccgaac gatgaaacag agaaactggc agaggccatt aatgcggagt
     5521 ttgctgatgc gtgccgtctt ggcaacatga ataaggcccg ctccgatgcc tatgcggaac
     5581 aaatcaaggc ggggctcagt tgggtggagg tcaggcgcaa cagcgatccg ttcggacctg
     5641 aattcagggt atccacagtc agcagaaatg aggttttctg ggactggctc agccgggaat
     5701 ccgacctgag tgactgccgg tggctgatgc gtcgccgctg gatggatacc gatgaggcaa
     5761 aagccacgtt cccgggaatg gctcaggtta tcgattatgc tattgatgac tggcgtggtt
     5821 ttgtcgatac cacgattact gaaggccagc ccagtccgtt gatgagtgca tgggaagagt
     5881 atcagtcatg ggatcgcagg gagaatgaat ggcttcagcg tgaacgccgt cgtgtgctgc
     5941 ttcaggtggt ttattaccgt acattcgaac gtcttccggt gattgaactc agtaatggac
     6001 gggtggtggc ttttgataaa aataatctga tgcaggcggt ggctgtggca tccgggcggg
     6061 ttcaggtgaa agtcgggcgg gtaagtcgta ttcgtgaagc ctggtttgtg ggcccacact
     6121 ttattgtgga tcgcccctgt agtgctccgc aggggatgtt tccgctggtt cctttctggg
     6181 gataccgaaa ggataaaacc ggggagccat acgggttaat ttcccgcgcc attccggcac
     6241 aggatgaggt gaattttcgt cgtatcaagc tgacctggct gcttcaggcc aaacgcgtga
     6301 ttatggacga ggatgccacc cagttgtcag acagcgacct gatggagcag atcgaacgtc
     6361 cggatggcat tattaaactg aatccggccc gaaaaaatca gaaaagtgtc gcagatgttt
     6421 ttcgggttga gcaggatttt caggttgcca gccagcagtt tcaggtcatg caggaatcgg
     6481 aaaaacttat ccaggatacc atgggagtgt attccgcatt tctcgggcag gattcaggtg
     6541 cgacgtcagg cgtggctatc agtaacctgg tggagcaggg ggccacaact cttgcggaaa
     6601 tcaacgataa ctaccagttt gcctgccagc aggtgggaag actgttgctg gcttatcttc
     6661 tcgatgacct gaaaaaacgc cgtaatcatg cagtggtgat taatcgcgat gatcgccagc
     6721 gtcgccagac cattgtcctc aatgctgaag gtgataatgg tgaactgacc aatgatattt
     6781 caaggttaaa tacacatatt gcgctggcgc ctgttcagca gacaccggcg tttaaggcac
     6841 agcttgcaca gagaatgtca gaggttattc aggggctgcc gcctcaggtg caggctgttg
     6901 tgctcgacct gtgggttaat cttctggatg tgccgcagaa acaggagttt gttgagcgta
     6961 ttcgtgctgc gctggggacg ccaaaatcac cggatgaaat gacgccggaa gaacaggaag
     7021 tagcggcaca acaacaggca cttcagcaac aacaggcaga actccagatg cgcgagatgg
     7081 ctggcagagt ggcaaaactg gaagctgacg ccgccagggc acatgcagct gcacaacggg
     7141 ataatgccag tgcacagcgg gaagtcgccc tgacacaggg gcagcgttat gtggatgcgc
     7201 ttaaccaggc acatacggca gaaatcatta ccggcgtaca gaatatggaa caggagcagg
     7261 acgttcttca gcaacagatg ctgtatacgt tacaacagcg gatgaatgaa atgtcgctct
     7321 gaaaactctg gcttcaactg aaccccgtca tcgtacgggg ttttttgttt ccggaggtaa
     7381 gcgttccggg agcggtgcgc ttattcgcgg gggcagcgat aagccttatt tactcaacca
     7441 ttcggatctg tccgataaac agaccatgcg gagttattta tggattttga atttacgggt
     7501 gaagaaaccc cggaacaact ggaaaaaatg ctggaaggac ttggggatgt ggatattgac
     7561 agtcacgcac aggacgtcgt gacggaagat accacggaaa aacatgcgga tgaggaagca
     7621 cagactcaga cgggcgataa caatgtggca ccgacgccgg atgccagtgt ggagcagacg
     7681 caggacgtga aggagccgga agcgaagggg gtgctcaccc gcgacggtaa acacgtcatt
     7741 ccctatgaag tccttgaggc tgaacgttcc ggtaagcaac gggccgaaca ggaagccgca
     7801 cttcttcgtg ggcagatagc tgaagaaaaa cgcagggtgg aactgctgac gtctcagatc
     7861 caccaggccg gtatgaagcc cacaccgtta ccggaaaacg aaaaaatttc tgatgagcag
     7921 attgcccgta tcagggagat gtatccggaa attggtgacg cggtggcttc gctcatccgt
     7981 aaaaataact atctccagtc ccgtgttcag caatcaacac agcaggcaga aggtaatggt
     8041 ggtgaggatt tatcaccggt tcttgatgcg atgaatgccg tgccggtgct gaaaacgtgg
     8101 caggagtccg atccggatcg cttctcggtt gctgtatcca tcgacgggaa gctccagaat
     8161 gaccccgcat ggaaagacaa aacgctcact gaacgtttcg ctgaagtggc ccgtcgtacg
     8221 caggttgctt tcggtgaagt cagtgagtcg tctgctgaca accaggcaga caaaacggat
     8281 atccggaaaa cggcggaaga aaaagtgaag accgctgaac aggagcaggc agtacctgct
     8341 tccccgtcag atttaggcac cacggcctcc gtcggaaccg gtgataattt tgaacggtta
     8401 cttggtgctt ctcattcaga ggctgaggcg attatgcgtg gtatgacgaa tgctgaaata
     8461 gacgcacttc tggagaagct cgggtaactt actgaaggag tactgaagta atgacgactg
     8521 taacatcagc ccaggcgaat aagctgtatc aggtggcgct ttttactgct gccaaccgca
     8581 accgctcgat ggttaatatc cttactgaac agcaggaagc gccaaaagcg gtttcgccgg
     8641 acaagaaaag cacgaagcag accagcgcag gtgcgccggt tgtccgtatc acagacctta
     8701 acaaacaggc cggtgatgaa gtgaccttca gcatcatgca caaactctca aaacgtccga
     8761 cgatgggaga tgagcgtgtt gaaggtcgtg gtgaggatct cagccatgct gacttctccc
     8821 tgaaaatcaa tcagggacgt cacctggtgg atgcaggcgg acgtatgagt cagcagcgca
     8881 cgaagtttaa cctggcatcc tctgccagaa cgcttctggg gacgtacttt aatgacctgc
     8941 aggaccagtg tgcgatagtg catcttgccg gagcacgtgg tgattttgtt gctgacgaca
     9001 ctattctgcc gacagcggag caccctgaat tcaaaaaaat catgatcaac gatgtactgc
     9061 ctccgacaca tgaccgtcac ttttttggcg gtgatgcgac aagctttgag cagattgaag
     9121 cggcagatat tttttctatt ggcctggtgg acaatctctc cctgttcatt gacgaaatgg
     9181 cgcatccgtt acagccggtt cgtctgtccg gtgatgaact tcacggagaa gatccatatt
     9241 acgtcctgta cgtcacgccg cgtcagtgga atgactggta cacctcgacg tccggtaagg
     9301 actggaacca gatgatggtt cgtgccgtga accgtgcaaa aggttttaat catccgctgt
     9361 tcaaaggtga atgtgcgatg tggcgcaata tcctggttcg taagtatgcg ggtatgccga
     9421 tccgtttcta tcaggggtca aaggttctgg tatcagagaa taacctgacg gcaaccacga
     9481 aagaggtcgc tgctgcaacc aatattgacc gcgccatgtt actgggggct caggcgctgg
     9541 caaatgctta cggtcagaag gcgggcggtc acttcaacat ggttgagaag aaaacggata
     9601 tggataaccg tactgagata gcaatcagct ggatcaacgg tctgaaaaaa atccgtttcc
     9661 ccgagaagag cggcaagatg caggatcacg gcgtgattgc cgttgataca gcagtgaagc
     9721 tctgattttt tcctttcccc atgccgggtt ttcgcccggc tttttcagga gtcattaatt
     9781 atggcaaaga ctatccttgc cccgtcactg agtgaacggg tctatacggg tacgcacggt
     9841 aatgagtcgg tggcagaagg cgtatttacg gtgaatgctg cggaagcgga cagtgttatt
     9901 catcttctct cactgccagt gggcatccgt atcaactcac tccagctggt ttcaacgggt
     9961 ggtctgggta ctgcaaccgt cagcattaag tccggtgagc atgctctcat cgataacagc
    10021 gaagctgttt ctgcaaaatt tgccagatat gtgccagtgg agccgtacac cacacagcgt
    10081 gacggggagc tggttactgt caccattaag actgccgctg caaccggcac cctgaatgtt
    10141 ctgctgcgtt ataccgtggt gggatactga ttaaaacctt ccggcccgcg tcatgcgggc
    10201 tttttatccg gggaattata tgagtgagaa aattgccgtt gtctatatcg gcccaaaacc
    10261 cgtgaaaaag gacaccatta ccggaagccg cacattgttc ccacgtcttg agccggtgca
    10321 tgttgacagt gcgatggcct ggcaactgct ggggtttccg gatgtctggg ttcgtcatga
    10381 agagcttgat gatgttctga aaaagcaaca acagaatgag cagttgcggc aggcacagca
    10441 ggcgcaggaa agcgtgcttg ctgcgcgggc agaagcggag aacagttttg ttgtttctgt
    10501 taacgggcag gaggtggatt taagtaagct cacctcagca cggctggcga cgctgtgtga
    10561 ggcagaagag ctggatattc acaaagaccc gaaagaaacg gctgaggcat tccgtatccg
    10621 ggtgcgtgag gcatttcgcc gtcgtgttgc ggagactgaa cagcatggcg gaactgagtg
    10681 attttttacc gtatgtccgt cgtcatatca gcggtccact gaacattatg atgacggatg
    10741 ctctgtcaat ggctgccgtg gcattcagcc gccagtcgtt ggtgtgccgt cgggaggtta
    10801 ctgttgtacc ggtggcagga aaagaaatcg tgcttccgta tgacaaagat gatgaggagt
    10861 gcgttcatat catccgtatc tctgacgata atcatgagct ttttgtcggt cgggatgtgg
    10921 atatcagctc cggacgctcc ctgcgatttg cctgttctcc cggtgaggtg agcgtgcttt
    10981 atgccgtcgc tccgaaagcc ggacgcagcc agataccgga tgaactcctc acatggcctg
    11041 aagaagtggc tgcgggggca cttgagcggt tgttcatgca gactggtgtt tcatggtcag
    11101 atccgttacg cgcacagtat ttttctgtgc agttttctga ggggatccgt cgggcatatc
    11161 gtcatacact ggcgacaagc ccgtactctt cataccgcaa ccctgtacgc aggcagaggt
    11221 tttactgatg acgacgatta cagaaatcat cggacgtgtg aatacacaac tggttgaccc
    11281 gatgatggtt cgctggcccc tgcaggaatt gtgcgattat tacaatgatg ctgtgagggc
    11341 agtgattctg gcgagaccgg atgctggcgc aagcctggaa acaatcagtt gtgttcctgg
    11401 cgcccgtcag gttttgcccg atggtgtaat acaacttctt gacgtgatat gcctcagtga
    11461 tggtagtgca gtcagaccat tatcccggga ggtgctggat gcgcagtatc ctgagtggcc
    11521 cacaatgaag ggcattcctg aatgttttat cagcaacgac ctgtccccgc gcgtattctg
    11581 gctgtttcct gtccctgaca aagagataag tattgatgca gtggtaagcc ggataccgga
    11641 ggcagtgtat gttctgacgc aggacgatga tacgccagtt ccactggaag aggcttatgt
    11701 taacccactg gtggactgga tgttgtttcg tgctttcagt aaggatgctg ccggtggtgc
    11761 agaatcgggg ctggctgcgc agcattatca gagttttgtt gagcaacttg ggatcaaaca
    11821 gggggcagac agtgcattgt ctgcccgtaa aaaagtgttt aacggaggtg gagtgtgagt
    11881 gttgttgttt cggggacgct gaaatctcct gatggtgagg cgatatcagg agcaaatatt
    11941 accctgacgg cgctgacagt ttcaccggat gcgctcagcg gcaccagtgc gtcggcagtg
    12001 acccgtgaag gtggatatta cggaatgacg atggatccgg gggagtatgc ggtttcggtg
    12061 acggtgaaag ggaagactgc tgtctacgga cgtgtgcgta ttgaggggac cgaaagtacg
    12121 gtgacgctca atatgctgtt acgccgcagt cttgttgagg tgagcatacc cggagagctg
    12181 ctgacagatt tccggcagat acagaataat gtggctgatg accttgctac tattcgtcgc
    12241 ctgaatgaag acacggcgac aaaaaacact caggccacac agtcaaaaga aagtgcagca
    12301 gccagtgcga agagtgcatc tgacagtgca aagacggcaa ccagcagggc ggctgaagcc
    12361 ggacaaaaag ccagtgaagc tgcagaagca gcgacccgtg caatcacagc agcggggaat
    12421 gcagaggaaa gctcgacccg tgccggagag tctgaaaaag ccgccggagc tgatgcagaa
    12481 aaagccagac tgcatgctga aaaggccagg ctggcgcagg agagcgccgg agagatcctt
    12541 aagcgggcag aggctgccac tgtcagtgct gaagaggcca gacgtatggc tgagaatgca
    12601 cgggggcccc gggggcctca gggagaaact ggtccgaagg gggatgtcgg tcctaaaggc
    12661 gaaacaggtc cagtgggccc tcaagggccc gcagggccga aaggtgagcg tggtgacgtt
    12721 ggtgctcagg gggcagtagg gcccgctggt ccgcggggtg agaagggcga acagggggag
    12781 cgaggaccgc agggaatacc aggcctgaag ggggataccg gagagcgggg gcctaaaggg
    12841 gaccaggggg atatggggcc aaaaggcgag aaaggtgatc cgggaggtcc tgcaggcccg
    12901 caaggtccta aaggtgaacg aggagaagcc ggaccacagg gaccgatggg agcacgaggt
    12961 gagcgtgggg agactggccc ccgaggtgaa cctggtcctg caggtccgag aggcgaacga
    13021 ggagagaccg gacctcaggg acctcgtgga gagccaggtc cggcaggcag cgctgcaaat
    13081 gtggctgatg cgacgacggc acagaaggga attgtgcagt taagcagcgc aacggacagt
    13141 gatgatgaaa cgaaggcagc caccccgaaa gcggtgaaag cggcaatgga caaagcggac
    13201 ggatgcctgg agaaagcgaa aaacggtgac gatatcccgg ataaggtgaa gtttctgaac
    13261 accgtggggg cagccagagt atacgggcgg gacattcata cgggggccgg tgaatggacc
    13321 acgagcgagt ttgtggcctg gctgaaagaa aagggggcat ttgaccagcc ttactggatg
    13381 atgaaggcat cactgcttgc ggaatttaat aaggtcatca cggatgtcgg accgggaaaa
    13441 ctcaatctgg gaggctgcgc cattgaggtg atggggacgt ataacgcagc catagtccgg
    13501 gtcaccatcg gcgaatacgg tggcgatggt tttctgaacg gcacggtctg tacctgtaca
    13561 gtttacggag acacacaacg ttttcactgg cgggtggatt acagcacaaa aaacaagccc
    13621 acaacagcaa gcctgacagt aaatggctgg gagcgtgatg aagtaaccgg tcgtttgcgg
    13681 caatggggca gtattgaggt aagtgaggat gatggtaagc ttatgacaca gcactctatc
    13741 cgttttcctg tcgcttttcc ggttgctgct ctgaatgctc aggtgtctgc agtgggagac
    13801 ccccacgcca ttaagatgta taccctgtcg aatccgtcat tagtgtctgt aactctgata
    13861 acagtcaggg gatgctacgg caagttttca tgggaagcga tcggatactg agggcattgt
    13921 atggaacagt atgttttttc accgtcagag aatatgttct atcccctgtc cctgcggccg
    13981 gtttatgagg cagcagggcg ctggccggaa gacggtattg tggtggatta tgtggtgtat
    14041 aaagtctttg cagcggatgc tgcaccggca ggtatgaagc ggggcgtcgg tgcggaaaaa
    14101 atgccggtct gggtgccggt atcggaggag ggcacggaaa catgatgcat atcagaaatt
    14161 tttcgtatta caccccggcg gagccggatg ttgcaggcgc gatgtacctg aagtctgagg
    14221 atggtcagga ctggtatgag tgtcaggcgt tgttttcgcc ggagacgctg aaggtggtgt
    14281 atgacagccg gggtgtgatt acgggatacg gtaaggacac tgcgttactg tggccggtga
    14341 accagagtgt ggcagaagtg ccggataccc cggagagccg gaaaattgac ctgagcgggc
    14401 gctggggttt tgacggggag aaaatcacgg acctgctgac cgcggagaaa gcgcgaggga
    14461 tgaagggcga tgaaataaac gcgtggcgta atgcgatgga agcggcgaac tacacgtttg
    14521 agcacaatgg gcgtaaatgg gactacggga agtcaacgca gacgcgtctt gagccgtcgg
    14581 tggcggcagc gaaatcgggg aaactgccgg aggcgttttt ctggacggat gcggaaaaca
    14641 atgatgtgga ggtgacagca gaagagctta tagcgctgag tgaagcggca gagcaggcga
    14701 tgttcaccaa agggatggaa atccacgttc g
//
LOCUS       DAZUCC010000126        14715 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZUCC010000126 DAZUCC010000000
VERSION     DAZUCC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.127"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(277..352)
                     /locus_tag="ACHY09_003244"
     tRNA            complement(277..352)
                     /locus_tag="ACHY09_003244"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Phe,seq:gaa)
     gene            complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHY09_003245"
     CDS             complement(458..1165)
                     /gene="yqgA"
                     /locus_tag="ACHY09_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS3886181.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1318..1395
                     /locus_tag="ACHY09_003246"
     CDS             1318..1395
                     /locus_tag="ACHY09_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886182.1"
                     /translation="MHATSLHSLAYRRFARNSSLFLLMR"
     gene            1558..3693
                     /gene="speC"
                     /locus_tag="ACHY09_003247"
     CDS             1558..3693
                     /gene="speC"
                     /locus_tag="ACHY09_003247"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFS3886183.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAAEQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLFGYVLK"
     gene            complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHY09_003248"
     CDS             complement(3743..4999)
                     /gene="nupG"
                     /locus_tag="ACHY09_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFS3886184.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHY09_003249"
     CDS             complement(5201..6280)
                     /gene="mltC"
                     /locus_tag="ACHY09_003249"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFS3886185.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHY09_003250"
     CDS             complement(6345..6620)
                     /gene="yggX"
                     /locus_tag="ACHY09_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFS3886186.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHY09_003251"
     CDS             complement(6648..7700)
                     /gene="mutY"
                     /locus_tag="ACHY09_003251"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS3886187.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7861..8580
                     /gene="trmB"
                     /locus_tag="ACHY09_003252"
     CDS             7861..8580
                     /gene="trmB"
                     /locus_tag="ACHY09_003252"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS3886188.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8580..8906
                     /gene="yggL"
                     /locus_tag="ACHY09_003253"
     CDS             8580..8906
                     /gene="yggL"
                     /locus_tag="ACHY09_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFS3886189.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            9090..9809
                     /gene="yggN"
                     /locus_tag="ACHY09_003254"
     CDS             9090..9809
                     /gene="yggN"
                     /locus_tag="ACHY09_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFS3886190.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9985..11031
                     /gene="ansB"
                     /locus_tag="ACHY09_003255"
     CDS             9985..11031
                     /gene="ansB"
                     /locus_tag="ACHY09_003255"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFS3886191.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKSVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDISKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11148..12155
                     /gene="yggM"
                     /locus_tag="ACHY09_003256"
     CDS             11148..12155
                     /gene="yggM"
                     /locus_tag="ACHY09_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFS3886192.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPIKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSADCYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQTNERWKKDTEQLRRNGVI
                     K"
     gene            complement(12211..13512)
                     /locus_tag="ACHY09_003257"
     CDS             complement(12211..13512)
                     /locus_tag="ACHY09_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS3886193.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLIALMMITYIPQISLFIPQLLGLM"
     gene            complement(13512..14015)
                     /locus_tag="ACHY09_003258"
     CDS             complement(13512..14015)
                     /locus_tag="ACHY09_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS3886194.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEVS"
     gene            complement(14060..>14715)
                     /locus_tag="ACHY09_003259"
     CDS             complement(14060..>14715)
                     /locus_tag="ACHY09_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS3886195.1"
                     /translation="LFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYA
                     KKPITKPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNV
                     PSWVQTRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQK
                     LWEKIDADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         3887 a         3539 c         3765 g         3524 t
ORIGIN      
        1 cagacactgg aagatgccct gagtttgtat acagatgagc gcacagtgag caagatttgc
       61 aaagggggga ataaatcttg acaggctgaa tcagcgatgt aagcatacag tgttaacgag
      121 aacagggcgt ctccgcaaaa gacaccctgt ttgaggatac gataccgaac tcaatcagtg
      181 taaccacaac ttacacaaac ggagaccctt tgatgctgca tcctgtatta accgcccgtc
      241 aaggatctca tcccttaacc agcgtgttct ggtatatggt gcccggaggc ggaatcgaac
      301 caccgacacg gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc
      361 gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc
      421 acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg
      481 cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt
      541 taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt
      601 ctgccatcat cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta
      661 actggatgat cagtaatggg atactaatta ccgacaccgc aatacccaac gagcaggcga
      721 agatcatcgc cgtaaagaaa tcgagaaatg acttggcgat taaaatactc ggatcgccgg
      781 tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa
      841 tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag tggcgaaaca
      901 gattttgtgc tttggtgacc gcggtattga cgcctttttc cagcagacaa atttcgccga
      961 ttaatgcacc gagtaaggtt gctaaaacca tcgccggaag gttggcgcat ttcaccacca
     1021 gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac
     1081 gttccggtaa acgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag
     1141 cgttgataaa agggccgatg accacaagag ttcctgttaa ctgtattgtc tgattttctt
     1201 attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgct
     1261 ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg
     1321 cacgccacct ccttacattc tctcgcttat cgccgtttcg cgcgaaactc ctcccttttt
     1381 ctgctcatgc ggtgaagtta acgcacgctc actgcaggac aacagtaaaa tcagagcgtt
     1441 tctgctttta ctgatgtctg gcggtcggag ctggtgacca gtttgaccca catctcatgg
     1501 ggcaggtttg tccatcctgt ccggtactct tacttccccg aaacgggttt tgcgcttatg
     1561 aaatcaatga atattgccgc cagtagtgaa ctggtatccc gactttctac tcatcgccgc
     1621 gtggtggcgt tgggagatac tgattttacg gacgtcgcgg cagtcgtcat taccgctgcg
     1681 gatagtcgca gtggcattct tgcgttgctt aagcgcaccg gttttcatct accggtgttt
     1741 ttgtattccg aacatgttgt tgaattacct gcgggcgtta cggcggtaat caacggcaac
     1801 gagcagcagt ggctggagct ggaatccgca gcctgtcagt atgaagagaa tttgctgcca
     1861 ccgttttatg acacgctgac gcagtacgtt gagatgggca acagcacctt tgcttgccct
     1921 ggacatcaac atggtgcgtt ttttaaaaag catcctgccg gacgccattt ttacgatttc
     1981 tttggtgaga acgtctttcg cgccgatatg tgtaacgctg acgtaaaatt gggcgatctg
     2041 cttattcatg aaggttcggc gaaggatgca cagaaattcg cagccaaagt ctttcatgcc
     2101 gataaaacct attttgtgct gaacggcaca tcggcagcga ataaagtggt gacgaatgcg
     2161 ctgttaacgc gtggcgatct ggtgctcttc gaccgtaaca accataagtc gaatcatcat
     2221 ggcgcgttga ttcaggcggg ggcgacgccg gtctatctgg aagcttcacg caacccgttt
     2281 ggtttcattg gtggtattga tgcgcactgt tttaatgaag agtatctgcg ccagcaaatt
     2341 cgcgacgttg cgccagaaaa agcagacctg ccgcgtccgt ttcgtctggc gattattcag
     2401 ctgggaacct atgacggcac tgtctataac gcccgtcagg tgatcgatac cgttgggcat
     2461 ctgtgtgatt acattctgtt tgattccgcg tgggtcggtt acgaacagtt tatcccgatg
     2521 atggcggata gctcgccgct gctgttagaa cttaacgaaa acgatccggg gatctttgtg
     2581 acgcaatcgg tgcataaaca gcaggcggga ttctcacaga cgtcgcagat ccataaaaaa
     2641 gataaccata ttcgcgggca ggcgcgtttt tgcccgcata agcggttaaa taatgccttt
     2701 atgctccatg cttctaccag cccgttctat ccgctgtttg ccgcgctgga cgttaacgcc
     2761 aaaattcatg aaggggagag tgggcgtcgg ctgtgggcgg agtgcgttgc gttggggatt
     2821 gaagcccgga aggcgattct tgcgcgctgt aagctgttcc gcccgtttat tccacccgtt
     2881 gttgatggca aattgtggca ggattatccg acgtcagtgt tagccagcga ccgtcgtttt
     2941 ttcagttttg agccgggggc gaagtggcac ggctttgaag gatatgccgc ggagcagtat
     3001 tttgtcgatc cgtgcaagct gttactgacc acaccaggta ttgatgctga aaccggtgaa
     3061 tatagcgact ttggcgttcc ggcgacgatt ctggcgcatt atctgcgtga gaacggtatt
     3121 gtgccggaga agtgcgatct caactccatt ctgttcttat taacgccggc ggaaagccac
     3181 gagaagctgg cacaactggt ggcgatgctg gcgcagtttg aacagcatat tgaggatgac
     3241 tcgccgctgg cggaggtgtt gccgagcgtt tataacaaat atccggtgcg ctatcgcgac
     3301 tacactttgc gccagttgtg tcaggagatg cacgatctgt atgtcagttt cgacgtcaaa
     3361 gacctacaaa aagcgatgtt ccgccagcag agtttcccgt cagtggtgat gaacccccag
     3421 gatgcgcata gcgcttatat tcgcggtgaa gtggagttgg tgcggattcg tgatgccgaa
     3481 gggcgaattg cggcagaagg ggcgttgcct tatccaccag gcgtgctttg cgtggtaccc
     3541 ggagaagtct ggggtggggc ggttcagcgt tatttccttg cgctggaaga aggggtgaat
     3601 ttattgccgg gattttcgcc ggagctgcaa ggtgtttata gcgaaaccga tgcggatggc
     3661 atgaaacggt tgttcggtta tgtgttgaag taagaataaa aaaaacgggt caccttctgg
     3721 cgacccgttt ttctttgcgt aattagtggc taaccgtctg tgtgcctgtc gggacacgaa
     3781 cgtgtttata tttgaacatc gccatgaacg cgaaggccag aaccacggag taaccagcga
     3841 aaatcagcca tacggtctgc cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt
     3901 taccgctcac gatgccgccg aggatacagc cgaagccgtt agtcatcatc aggaacatcc
     3961 cctgcgcact ggcgcgaatt gccgggctaa cttctttttc gacaaacacc gaaccagaga
     4021 tgttgaagaa gtcgaatgcg caaccgtaaa cgatcatcga cagtaccagc agtacagtac
     4081 cgaacggagt cgggtcgccg taagcaaaca gcgcaaaacg caggatccac gccacaatac
     4141 tgatcatcat tacgttctta ataccgtagc ggcttaagaa gaacgggatg gtcagaatga
     4201 acagggtttc agagatctgc gaaatcgaca tgatgattga cgcatgctgc acgatgaagc
     4261 tgctggcaaa catcggatct ttgtcgaagc tgtgcaggaa ggtgttaccg aacatgttgg
     4321 taatctgcag ttccgcgccc agcagcattg agaagatgaa gaagattgcc atacgcttgt
     4381 ttttaaacag cgcgaatgca tcgaggccca gcagggttgt ccagctctga ttcgcttgct
     4441 gtttagcaac cggaatatgc ggcagagtca gggtaaacag aaccagaatg gcggaaagtg
     4501 ctgcgccaat atacagctgc atgtggctta attcgaagcc agacaggctc accacccaca
     4561 ttgccatgat aaagccgatg gtgccccaga tacggattgg cgggaagtca gtaacgatat
     4621 ccatcccggc attttgcagg cgatagtaag agatggtgtt gattaaccca agcgttggca
     4681 tataagcaaa cgagttaatc aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg
     4741 ccgccatgaa cagcgtgata gcgccaatgg tgtggcaaat ggcatatacc catttcgcac
     4801 ttaaccattt gtcggccaca atccccagca gcgcaggcat aaataccgcg gcgataccca
     4861 gtgagctata aactgcgcca atagaagcac cgtcaaactt cagggtaaca aacatatagg
     4921 agccgagggt cgtcagccaa cttccccaca gacagaactg cagaaaagag aggattttca
     4981 gctgcagctt aagattcatg ttaatttcct cacatcgtga tgcggatgag tgtttgaaaa
     5041 agcacatttg accgtgcgag atgatgggat tctatcaact tcccggactg gttttttgtt
     5101 acctgtggca aataattgca aacattccca atttgcaaaa cgaaattgtg atgtggataa
     5161 catttttacc cctgagcatc gtcaggggcg gttagtggaa ttatcggcgg cggtaggatt
     5221 tttgcgcggt attcacttta taaagataac ggcgagattc cgcagaggga tggcgggtcg
     5281 tcagcgtttg ataaacatcg cccggcgtca tggtgttaat aatattggcc gcctgaatct
     5341 tatcattcga aaagactcgc agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg
     5401 cataacgccg ggatgtaggg ttatcaattc cgccgagata aacgttgttc agcatcgcca
     5461 gatacgcggt gccggtatca atattgctgg caggatcaaa caagaaactg cggctcggcg
     5521 tgccggattt cccctgcgaa cggaacacat ctttcccggc agtatgttgt accacctgca
     5581 ttaaccccag cgcatcggaa cggctgaccg catacgggtt aaaggatgac tcggtctgca
     5641 taatcgccag aatcagcgac tcatcaacgc catatttccg tgacgcctgg cggaccatgc
     5701 cgagatattt gtgcgcacgt ttatcaaggt ggttcggcac catgttaatg gtgacgctgt
     5761 agatgatacg cagcccgttg ctgcggctct tcagacggtt tttcagcaga taatccgcga
     5821 agttgctcgc gcgaccttcc cagcgaatcg gctgcccggt gttgtccacc acctgaccgt
     5881 aaaggaaagg ttctttcgaa atcgtaatat catcaacgtc ggaatagaga tcgaccgaac
     5941 tcggatcgtc ccccatcagc aacgttttga taattgcccg gcgcagatgc gcggcaggtt
     6001 ctgtcccggc gatggtttcg atagtaatcg taccgtcatc gaagttgatg tggctgcggg
     6061 tctgatattg atcggtgtat ttcacgtagt ccttaggacc ggcgatcacc acctctttga
     6121 agccccagat gttctcaata ttgtgggcaa attgccccat cagaatatca aaaccgttgg
     6181 tatctttgac ccaggcttcg ttataggtat cgcctttttt ggtcgtcgaa caggagatga
     6241 gcaacggcgc aatcaaagcc agcgcgagat attttttcat cattccgggt gcgtgttgtg
     6301 tttgttttta tgcaagttgc cggaggcgtg ctccggcact gtttttattt tttatcttcc
     6361 ggcgtatagc cctcaatatg cacctcttta ccctcgaaca ggaagttgac catctcctgt
     6421 tccagcagct tgcggtgctc ggcattcatc atgttgagtt tcttttcatt aatcagcatg
     6481 gtttgtttgt gctgccactg cgcccaggct tctttggaga tctcgttata gatgcgtttt
     6541 cccagctcgc cggggtacag ctgaaaatcc tgaccttctg cttcacgttg caggaaagta
     6601 caaaaaatcg ttctgctcat aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg
     6661 ccagtgcgta actgctgtaa caaacgctcc acgggagccg ccaggccaac tgacggcggt
     6721 tgcgctaagt tataccagag cgcattgcct tcatccatgc agccggtgaa tgacgacacg
     6781 ggaagccaca taggcacaat atctaagtgg aaatggctga aggtatgccg aaacgcggtc
     6841 agctgcgtca ggttatcggc agcaatctgc cgttgcgcca gccactgccg caaactttct
     6901 tcgtcggcaa actgcgggaa acagtataaa ccgccccaca atccgctcgg cggacgctgc
     6961 gccagcaata cttcatcttc gtgctgtaac aggagaaagt agccggtgcg ctccggcagc
     7021 gtctgtttcg gttttttgcc cggataaagc gaccagctat tgttggtggc ggcaatacat
     7081 ccgttttgta gcggacagag cgagcatttc ggcttcgagc gcgtgcaaat catcgcaccc
     7141 aaatccatca tcgcctgatt aaaccgctcc acgccgaccg cgggcgtcac ctgttcgctc
     7201 aaactccaca gtttattctc gacctctttt ttcccaggcc agccgcttac agcatagcag
     7261 cgcgccagca cccgtttgac gttaccgtcg agaatcggaa agtgcttacc cagagaaagc
     7321 gagagaattg cgcctgcggt ggaacgcccg acgcccggta acgccgcgac ttcttcaaag
     7381 gtttccggga atttaccgcc gtgtaaggtc gccacttgtt gtgccgcctt atgcagattg
     7441 cgcgcgcggg cgtaatagcc aagcccggtc cacaagtgga gaacttcgtc gagcggcgca
     7501 ttggcgagat cggtcaccgt cgggaagcgc gccataaagc gttcaaaata ggggataacg
     7561 gtcgcaacct gagtttgttg caacatcact tctgagagcc atactttgta gggcgtcttg
     7621 tcaatttgcc agggcagcgt tttccgcccg tatttatcgt accagtccag aacctgggct
     7681 gaaaattgcg acgcttgcat ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca
     7741 cagcgacggc agggtgtaaa ccggaacttt ccgcagccac ggccttatca tgcttgcatc
     7801 cggcaactaa ctttggataa tgcccgtttt cagaacactt tcacaagcga ctaaatcttt
     7861 atgaaaaacg acgtcatctc accggaattt gatgaaaacg gccgcccact gcgccgtatc
     7921 cgtagttttg tgcgccgcca ggggcgactg accaaaggcc aggaacatgc gctggaaaac
     7981 tactggccgg tgatgggcgt tgagttcagc gaagatatgc tggatttccc cgcgcttttt
     8041 ggccgagaag cgccggtgac gcttgagatt ggtttcggca tgggggcgtc gctggtggca
     8101 atggctaaag atcgccccga gcaggacttc ctcggcattg aagtgcattc accgggcgtt
     8161 ggtgcgtgcc tggcttctgc gcatgaagag ggcttaagca acctgcgcgt gatgtgtcac
     8221 gatgcggttg aagtgctgca taaaatgatt cctgacaatt cattgcgcat ggtgcagctc
     8281 tttttccctg acccgtggca caaagcgcgc cataataaac gccgtatcgt tcaggtgccg
     8341 tttgccgaac tggtaaaaag caaactgcaa ctggggggcg tattccatat ggcgaccgac
     8401 tgggaacctt atgcggaaca tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac
     8461 ctgtcagaga gcaatgatta cgtaccgcgt ccggcatcac gtccggtgac gaaatttgaa
     8521 caacgtggtc atcgtcttgg tcacggagta tgggacttaa tgttcgagag ggtgaaataa
     8581 tggcaaagaa ccgtagccgt cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat
     8641 taggattttc ggtggcatgg cgattcccgg aaggtacatc ggaagaacag attgataaaa
     8701 ccgttgatga ctttattaac gaggttatcg aaccgaacaa actggccttt gacggcagcg
     8761 gttatctggc ctgggaaggt ctgatctgca tgcaggaaat cggcaaatgc accgaagaac
     8821 atcaggcgat tgtgcgtaag tggctggaag agcgcaaact gggcgaggta cgcaccagcg
     8881 aacttttcga cgtttggtgg gactaagaaa gcatacgggc gatgacaaat gcaaaactgc
     8941 ctgatgtgct acgcttatca ggcctataaa gatgcacgat cgagtaggct ggataaggcg
     9001 tttacgccgc atccggcatg gaaaacgcgc actttgttat caatctgggg ccagcaaatg
     9061 ctggcctgat ttgttcttga gggaagacta tgatgcgcaa aatgctgctg gcggcagcac
     9121 tttcagtgac ggcaatgacc gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg
     9181 atgtgattgt cagcccgcaa accgtgcagg tgaagggcga aaacggcaat ctggtgatca
     9241 cgccagacgg caacgtgatg tataacggta agcaatattc cctgaatgcc gcccagcgcg
     9301 agcaggcgaa ggattatcag gctgaactcc gtagcaccct gccgtggatt gatgaaggcg
     9361 cgaaaagccg cgtcgaaaaa gcccgtattg cgctggataa aattatcgtt caggagatgg
     9421 gcgaaagcag caaaatgcgc agccgtctga ccaaacttga tgcgcagctg aaagagcaga
     9481 tgaaccgcat tatcgaaacg cgcagcgatg gcctgacgtt tcactataaa gccattgatc
     9541 aggttcgcgc cgaaggccag caattagtga atcaggcaat gggtggaatt ttacaggaca
     9601 gcattaatga aatgggcgcg aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg
     9661 tgctgggaag cctgggcggt ctgcaatcct caatccaaac cgagtggaaa aagcaggaaa
     9721 aagatttcca gcagtttggc aaagatgttt gtagccgcgt tgtgactctg gaagatagcc
     9781 gcaaagccct ggtcgggaat ttaaaataat cctctatttt aagacggcat aatacttttt
     9841 tatgccgttt aattcttcgt tttgttacct gcctctaact ttgtagatct ccaaaatata
     9901 ttcacgttgt aaattgttta acgtcaaatt tcccatacaa agctaaggga taatgcgtag
     9961 cgttcacgta actggaggaa tgaaatggag tttttcaaaa agacggcact tgccgcactg
    10021 gttatgggtt ttagtggtgc agcattggca ttacccaata tcaccatttt agcaactggc
    10081 gggaccattg ccggtggtgg tgactccgca accaaatcta actacacagc gggtaaagtt
    10141 ggcgtagaaa atctggttaa tgcggtgccg caactgaagg acattgcgaa cgttaaaggc
    10201 gagcaggtag tgaatattgg ctcccaggac atgaacgatg atgtctggct gacactggcg
    10261 aaaaaaatta acaccgactg cgataaaacc gacggcttcg tcattaccca cggtaccgac
    10321 acgatggaag aaaccgctta cttcctcgac ctgacggtga aatgcgacaa atcggtggtg
    10381 atggtcggcg caatgcgccc gtccacgtcc atgagcgcag acggtccatt caacctgtat
    10441 aacgcggtag tgaccgcagc tgataaagcc tccgctaatc gtggcgtgct ggtggtaatg
    10501 aacgacaccg tactggacgg tcgcgatgtc accaaaacca ataccaccga cgtagcgacc
    10561 ttcaagtctg ttaactacgg tcctctggga tacattcaca acggtaagat tgactaccaa
    10621 cgtaccccgg cacgtaagca caccagcgat acgccgttcg atatctctaa gctgaatgag
    10681 ctgccgaaag tcggcatcgt ttataactac gctaacgcat ccgatcttcc ggctaaagca
    10741 ctggtagatg cgggctatga tggcattgtt agcgctggtg tgggtaacgg caacctgtat
    10801 aaaaccgtat ttgacactct ggcgaccgcc gcgaaaaacg gcactgcagt cgtgcgttct
    10861 tcccgcgtac cgacgggcgc taccactcag gatgccgaag tggatgatgc gaaatatggc
    10921 ttcgtggcct ctggcacgct gaacccgcaa aaagcgcgcg ttctgctgca actggctctg
    10981 acgcaaacca aagatccgca gcagatccag cagatcttca atcagtacta atcacctcgc
    11041 cccggtaacg tgccggggct ttttcacttc agacttacgt ccattgccaa ttttcattac
    11101 cctgatgata atcactggaa taaattattc cgcgcgaggg ttttcgggtg aaaaagcaat
    11161 ggattgttgg cacggcgctg cttatgttga tgactggtaa tgtctgggca gatggcgaac
    11221 cgccaactga aaatatctta aaagatcaat tcaaaaagca gtatcacggc attctcaagc
    11281 ttgatgtcat caccttaaaa aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg
    11341 aaggcgatgt ctcttccagt gacgatctct atacctgggt cggtcagttg gcagattacg
    11401 aactgctcga acagacctgg acgaaagata aaccgataaa attctcggcg atgttaacca
    11461 gtaaaggaac gcccgcgtct ggctggtcgg tgaactttta ctcttttcag gcggcagcca
    11521 gcgatcgtgg acgggtggtc gacgatatca aaacgaataa taaatatctg atcgtgaata
    11581 gcgaagattt caattatcgc tttagtcagc ttgaatcagc gttgaataac cagaagaact
    11641 cgattcctgc gctggaaaag gaagtgaaag cgcttgataa gcaaatggtg gcggcgcaga
    11701 aagcagcgga tgcatattgg ggaaaagatg caaacggtaa gcaaatgacc cgcgaagacg
    11761 cctttaaaaa aattcaccaa cagcgtgatg agtttaataa acagaacgat agcgaggcgt
    11821 tcgcggttaa atatgacaaa gaggtttatc aaccggcgat tgcggcatgt cataaacaga
    11881 gtgcagattg ttatgaagtg ccgattcagc agaagcgaga tttcgatatc aacgaacaac
    11941 ggcgacagac ttttctgcaa tcacaaaaac ttagccgtaa attgcaggat gactggataa
    12001 cgctcgaaaa agggcaatat ccgttaacga tgaaagtttc agaaataaac agtaaaaaag
    12061 tcgcgatttt gatgaaaatt gatgatatca accagactaa tgaacgctgg aaaaaagata
    12121 ccgaacaatt gcggcgcaat ggagtgatta agtaactcat gttaagtacc tttgccggat
    12181 aatataaaaa gttatccggc aaattatttt ttacattagc cctagtagct gtggaatgaa
    12241 caaggatatt tgcgggatgt aagtgatcat catcaacgcg attaacaatg cggcataaaa
    12301 tggcagcaat ggtttgatca aatgttgaat cttcacacct gaaatagaac aaccaacaaa
    12361 tagcgcacta cccaccggcg gtgttaacag cccaatacaa aggttagcca ccatcataat
    12421 accgaaatgt accggatcca ttcccagctc ttgtgcaatc ggcaagaaga ttggtgtgaa
    12481 gattagtaca gccggtgtca tgtccataaa gatgccgaca attaataaga cgatattaat
    12541 cagcagcaat atgaccagtg ggttatcaga aatccccatc aacgcgtcgc tgatcatata
    12601 aggaatatca gcattggtca ttgcccagga catgcccacg gaaaaaccga tcaacagtaa
    12661 tacaatcgaa gtggtaacaa ccgattccag aatcagtttg ggtaaatcgc gccattttac
    12721 ttcccggtaa atcagcaccg agagaataaa tgtgtagaca acggcaattg ctgacgcttc
    12781 agttgcggta aaaatgccgc ccagaatgcc gcccattaca atgaacacca gaagcaaact
    12841 tggtaaggca tccagaaacg ctttacatgc ctgagcaaag gttgctcttt ctgaaagtgg
    12901 gtacccacgg cgtttagcaa taatgccgca gactatcatt accgcgagac ccattaagat
    12961 ccctggcaaa tatcccgcca taaatagcga tgctacagaa acgccgccag ctgtcaggga
    13021 aaacacaatt aacacgtttg agggcggtat cagtaatcca gtaatacaag atgagacgtt
    13081 tacagcggta gaaaatgccg gatcataacc ttctttggtc tgaatgggat tcagtgtgcc
    13141 accaacggca gcagctgccg caacggctga accagaaatg gaaccaaaca tcatattcgc
    13201 caggacgttc acatgaccaa gcgaaccagg aacgcggcca accattacct gggcgaggtt
    13261 aatcaaacgt atcgcaatac cgccgctgtt catcagcgtt ccggcaaaga tgaaaaaggg
    13321 gattgccaat agggcaaagt tatccagtcc attagccagt cgctgcgaaa cagtgattgc
    13381 cgcgatatcc cagggaaaca tcaacagcat tgaagctaca gtggcaatac cgattgagaa
    13441 cgaaatgggc acaccaataa agaccagcac aaaaaaacag ccaaacagtg taagcgcgat
    13501 ataactatcc attaactgac ctcctgcgca ggttgtttca gttgttgaat gctttgatta
    13561 ataaaacaca aggcataaaa catcatcacc aatccactta atggcagaat gatatatacg
    13621 tatcccatcg gtatttgcat cgcggcagat acctgtgatg ttgccagtgt tttatcaatt
    13681 aatttcaggc caccagtcac aatgaccgaa ccggcaaaac ctaaaataag aacgttaatg
    13741 actatactaa gaagtaattg cttgcgctta ttcagggcga gagcaaataa atcaatagag
    13801 agatgtctct gcgcgccgac ggtatatgca gcacctaata atcctaccca gatcatcaaa
    13861 aagcgtgcca gctcatcggt taatgtactt ggctggttaa gtacgtagcg gctaaatact
    13921 tgccagacta cgcatacaac cagagcaagc attacggcga cagaaaacgc ggcaatgacg
    13981 cggtctaccg caagtttcag gcggttaaat atcattgtaa gcatcctgga aaatcagggc
    14041 cagagactgg cccgaaatat tattgagcag cgttgtcgaa tttctctaac aaagcagctt
    14101 gttttggatc ttttttgaag tcgtcaaaca gaggctgaac tgcggcacgg aatggggctt
    14161 tatcaacctt aacgatttca ccacccatgg ctttcgcctg agcacgtgtg tcagcgtcaa
    14221 ttttttccca caatttttgt tgataagctt cggatttctt cgccgctgtt tcgaggattt
    14281 gttgctgttc tggggttaat ttattccatg ttttggtgga gataaccaaa aagtcaggaa
    14341 ttgaggcatg ttcatcttca gaaaagactt tggcaatttc gatatgtcga gtctgcaccc
    14401 aggaaggcac gttattttct gcgccatcta ctacaccttg ctgcatcgca gtataaactt
    14461 cgccaaatga aattggagtg ggtgagccac ccatcagttc aatcattttg atggtggtcg
    14521 ggcttggctg aacacgaatt ttcagccctt taagatcttc tggcttagta atcggttttt
    14581 tcgcatagaa actgcgtgta cccgctacat aagctgataa agcaaaaaag cctttatctt
    14641 ttgttgaatc cataatttct ttaccaactt caccaaaaac cactttgttg aagtgcgcct
    14701 gatctttaaa taaga
//
LOCUS       DAZUCC010000127        14198 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZUCC010000127 DAZUCC010000000
VERSION     DAZUCC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.128"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            113..679
                     /locus_tag="ACHY09_003260"
     CDS             113..679
                     /locus_tag="ACHY09_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3886196.1"
                     /translation="MFKGQKTLAALAISLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            730..3189
                     /gene="ybgQ"
                     /locus_tag="ACHY09_003261"
     CDS             730..3189
                     /gene="ybgQ"
                     /locus_tag="ACHY09_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS3886197.1"
                     /translation="MDTVNIYRLSFISCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKGKGVIVPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRANNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATDSTISLA
                     AYRFSDRHFHSYANYLDHKYNNSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDNSGISVGMVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSLHLPEHMEDTANRLILPCH"
     gene            3204..3935
                     /gene="ybgP"
                     /locus_tag="ACHY09_003262"
     CDS             3204..3935
                     /gene="ybgP"
                     /locus_tag="ACHY09_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3886198.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPSPEKNSGHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVITNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            3932..4993
                     /gene="ybgO"
                     /locus_tag="ACHY09_003263"
     CDS             3932..4993
                     /gene="ybgO"
                     /locus_tag="ACHY09_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3886199.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGTSGGTVEKSEAIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREIPPHDYQSSLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLIEEDKALLIGNGLKLRLLDENSSPYTFNKYSEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            5146..6192
                     /gene="abrB"
                     /locus_tag="ACHY09_003264"
     CDS             5146..6192
                     /gene="abrB"
                     /locus_tag="ACHY09_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS3886200.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATNPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6189..6980)
                     /gene="nei"
                     /locus_tag="ACHY09_003265"
     CDS             complement(6189..6980)
                     /gene="nei"
                     /locus_tag="ACHY09_003265"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFS3886201.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKPYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTMRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLITHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7016..7750)
                     /gene="pxpA"
                     /locus_tag="ACHY09_003266"
     CDS             complement(7016..7750)
                     /gene="pxpA"
                     /locus_tag="ACHY09_003266"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFS3886202.1"
                     /translation="MKIDLNADLGEGYASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSVMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(7740..8672)
                     /gene="pxpC"
                     /locus_tag="ACHY09_003267"
     CDS             complement(7740..8672)
                     /gene="pxpC"
                     /locus_tag="ACHY09_003267"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFS3886203.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8666..9322)
                     /gene="pxpB"
                     /locus_tag="ACHY09_003268"
     CDS             complement(8666..9322)
                     /gene="pxpB"
                     /locus_tag="ACHY09_003268"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFS3886204.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGIYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9345..10088)
                     /gene="ybgI"
                     /locus_tag="ACHY09_003269"
     CDS             complement(9345..10088)
                     /gene="ybgI"
                     /locus_tag="ACHY09_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFS3886205.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10359..11840
                     /gene="dtpD"
                     /locus_tag="ACHY09_003270"
     CDS             10359..11840
                     /gene="dtpD"
                     /locus_tag="ACHY09_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFS3886206.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFSHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDIFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11883..13301)
                     /gene="phrB"
                     /locus_tag="ACHY09_003271"
     CDS             complement(11883..13301)
                     /gene="phrB"
                     /locus_tag="ACHY09_003271"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFS3886207.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSASVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLQIALAEKGIPLLFHEVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVQAERALRNVVCEGFDDGVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIDPAPSITLNYPRQPFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSIWLNEL
                     IWREFYRHLITYYPSLCKHRPFIVWTDRVQWQSNSAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13298..13807)
                     /gene="ybgA"
                     /locus_tag="ACHY09_003272"
     CDS             complement(13298..13807)
                     /gene="ybgA"
                     /locus_tag="ACHY09_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFS3886208.1"
                     /translation="MNQQRFDESTLIRIFALYELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRGGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13949..14198)
                     /locus_tag="ACHY09_003273"
                     /pseudo
     CDS             complement(13949..14198)
                     /locus_tag="ACHY09_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3422 a         3796 c         3451 g         3529 t
ORIGIN      
        1 tctttatata acaagctttt taatattgtt aaataaaacc aaatacattc aactgtcagt
       61 taatatattc ttgcctcgca acactctatc tcattattta aaggactcag ctatgtttaa
      121 aggacaaaaa acattggccg cactggccat atctctgcta ttcacagcac ctgtttatgc
      181 ggctgatgaa ggatccggtg aaattcactt taaaggtgaa gttattgaag caccgtgtga
      241 aatacatcag gatgatattg ataaagaggt tgaactcggt caggtgacca ccagccacat
      301 taatcagtcg catcacagcg atgccgttgc tgtcgacctg cgcttagtca actgcgatct
      361 ggaaaactcc aacaacggtt ccggcggcaa gatttctaaa gttgcagtca cctttgacag
      421 ctcagcgaaa accaccggcg cagatccgat tctcaacaac accagcacgg gtgaagcgac
      481 cggtgtcggc gtgcgtttaa tgaataaaga tcaaagcaat atcgttttag gcaccgctac
      541 tccagatatc gacctggcac caacctccag cgaacaaacg ttaaatttct ttgcgtggat
      601 ggaacaaatt gatcaggcta cgcccgtaac accaggcgca gttacggcga atgcgaccta
      661 cgtactggat tataaataag tatcactttg gcggcgtcat cgtcgccttt cttcaaatat
      721 ttatcaggta tggacaccgt gaatatttat cgactctctt ttatatcctg tctggtcatg
      781 gcgatgcctt gcgcattggc ggtcgaattc aacctgaatg tcctcgataa atcgatgcga
      841 gatcgcattg atatttcatt attaaaggga aaaggggtca ttgttcccgg tgaatatttt
      901 gttagcgttg cagtaaataa caaccaaatc agtaacggac aaaaaattga ctggaaaaag
      961 aacggtgacc aaaccattcc gtgtattaat gatttactgg tagataaatt tggtttaaaa
     1021 cctgaagttc gtcagtcgtt accacggtta aaccagtgcg tcgattttag ctcccggcct
     1081 gaaattctct tcatttttga tcaggctagc cagcaactga atatcactat tccacaagcc
     1141 tggctggcgt ggcactcaga taactggact cccccctcaa catggaaaga aggtgtcgcc
     1201 ggcgtcctga tggactacaa cctgtttgca agcagctacc gcccacagga tggtagcaac
     1261 agcactaacc tgaacgccta tggaacagcc ggaattaacg cgggagcatg gcgtttacgc
     1321 agtgattacc aattgaatca gaccgatagc gatgataacc atgaacagtc aggcgaaata
     1381 tcacgcacct atctttttcg tccattaccg caattaggtt caaaattaac ccttggtgaa
     1441 acggatttca gttccaatat tttcgacggc ttttcttata ccggcgcggc actggcaagt
     1501 gacgatcgga tgttaccgtg ggagctacgc ggctacgccc cacaaatcag cggcattgca
     1561 caaaccaacg ccaccgtcac cgtgagtcac tcagggcgag ttatttacca gaagaaagtc
     1621 ccaccaggtc cgtttatcat tgatgacctt aatcagtccg tccagggtac gttagatgtc
     1681 aaagtgacgg aagaagatgg tcgggcgaac aatttccagg tttcggcagc atcgacgcct
     1741 ttccttacgc gccaggggca ggttcgctat aaacttgctg ccggtcagcc gcgaacatct
     1801 atgtctcatc aaactgaaaa tgaaaccttt tttagcaatg aagtttcctg ggggatgctg
     1861 tcaaatacct cactgtacgg cggcctgctg atttcgggtg acgactacca ttctgccgca
     1921 atgggtatcg ggcaaaatat gctgtggctt ggtgcattgt cgtttgatgt cacgtgggcc
     1981 agtagtcagt ttgatactca gcaggacgag cggggcttaa gttaccgctt taattacagc
     2041 aaacaagtgg atgccaccga cagcacgatt tcgctcgccg cttatcgttt ctccgatcgt
     2101 cattttcaca gctacgccaa ctatctggat cacaaataca acaacagcga tgcgcaggac
     2161 gaaaaacaga cgatcagctt atccgtgggc caaccgatta ccccactgaa cctcaatctg
     2221 tacgccaacc tgctgcatca aacctggtgg aatgcagacg cctccacgac cgccaacatc
     2281 acggcgggtt ttaatgttga tattggtaac tggagagata tctcgatctc gacgtcattc
     2341 aatacaaccc attacgaaga taaagatcgc gacaatcaga tttacctgtc gatttcgctc
     2401 cccttcggta acggggggcg ggtcggttat gacatgcaaa acagtagtca cagcaccacg
     2461 caccgcatgt cgtggaatga tacgttagat gaacgtaata gctggggcat gtctaccgga
     2521 ctgcaatccg accgtccgga taatggagcc caggtgagcg gtaactatca gcacctgagt
     2581 tcagcgggtg agtgggatat ttctggtacc tatgccgcca atgattacag ttccgtcagc
     2641 agcagctgga gcggttcttt cacggcaact caatatggtg cggcgttcca tcgtcgcagc
     2701 tccaccaatg aaccacgtct gatggtcagt accgatggcg tggcagatat tccggttcag
     2761 ggcaatctcg actacaccaa ccattttggc attgcggtgg tgccgttgat ttccagttat
     2821 cagccttcca ccgtggcggt gaacatgaat gacttacccg acggcgtaac agtggcagaa
     2881 aacgttatca aagaaacgtg gattgaaggc gcgataggtt acaaatcgct ggcttcccgt
     2941 tccggtaaag acgttaacgt catcattcgc aacgccagcg gtcagttccc tcccctcggt
     3001 gcggatatcc gccaggataa cagcggcatt agcgtgggga tggttggcga ggaaggacat
     3061 gcctggttaa gcggagtcgc tgaaaatcaa caatttaccg tgatctgggg tgatagccag
     3121 cgttgctcgc tccatcttcc tgaacatatg gaagacaccg caaatcgcct gattttacct
     3181 tgtcattaat taaggaaatg aaaatgacat ttatgaaagg actacccctt ttgttgttgg
     3241 ttgccagtct gtgcagccac gctgcactac aacccgatcg cactcgtatt gtgtttaacg
     3301 ctaatgataa agctaccagt ctgcgcgtgg ataatcgtag cgataaactc ccctacctcg
     3361 cttattcctg gctcgaaaat gaaaaagggg agaaaagcga tgatcttctg gttgccctgc
     3421 caccgataca acgtctggaa ccgaaagcaa caacgcaggt acggatagta aagcaagcct
     3481 cgaccacaaa attaccgggc gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc
     3541 ctccctcccc agaaaaaaac agcggccatg ccgttctgca agtcgccatt cagagtcgta
     3601 tcaaggtatt ctggcgaccg gctgcattac gcaagaaagc aggagaaaag gtcgaactgc
     3661 agttacaggt cagccagcag ggcaaccaac tgacactgaa aaatcctacc gcgtattatc
     3721 tgaccattgc ctatctgggg agaaatgaaa aaggcgttct tcccggcttc aaaaccgtga
     3781 tggttgcgcc ttttagtaca gtaattacaa acaccggaag ttatagcggc agtcagtttt
     3841 atctcggtta tatggatgat tacggcgcgt tacgcatgac gacgctcaac tgcagcggac
     3901 aatgccgttt acaagcagtg gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt
     3961 catctgtctg gtatttctgc cattgaagtc tgcgctagcg ctgaactgct attttggtac
     4021 ttccggcgga acggtagaaa aatcggaagc gattcaaccc tttgctgtgc caggtaatgc
     4081 caaacccggc gataagatct gggaatctga cgatattaaa attcccgtct attgtgacaa
     4141 caataccaac ggcaactttg aaagcgaaca tgtctacgca tgggtaaacc cctacccggg
     4201 ggtacaggat cgttattatc aactcggcgt gacatacaat ggtgtcgatt acgatgccag
     4261 tctggggaaa agccgcatcg acaccaacca gtgtatcgac agtaagaaca ttaacattta
     4321 tacccctgag cagattatcg cgatgggctg gcagaacaaa atatgctcgg gtgaccccgc
     4381 taatattcac atgtcgcgaa cctttcttgc tcgtatgcgg ttatacgtta agattcgcga
     4441 aataccgccg catgattatc aaagttcgct tagcgactac atcgtcgtgc aatttgatgg
     4501 tgccggtagc gttaacgaag accctaccgc ccaaaacctg aaataccata ttactggtct
     4561 ggaaaacatt cgtgtactgg attgcagcgt caatttttct atttcaccag aaacacaggt
     4621 gattgatttt ggtaaattta acctgctgga tattcgccgc cacacaatgt cgaaaacatt
     4681 cagtattaag acgacaaaat cacaaaatga ccagtgcacc gacggattta aggtcagctc
     4741 ttcgttttat accgaagaaa cattgattga agaggataaa gcactgctga ttggtaatgg
     4801 actgaagcta cgtttgctgg atgaaaattc ttcaccctat actttcaaca aatacagcga
     4861 atatgctgat ttcaccagcg atatgctgat ctacgaaaaa acctatacgg ctgaactttc
     4921 gtccattccc ggcaccccca tcgaagcggg tcccttcgat acagtggtgc tttttaagat
     4981 taactataac taatttatca tgatgccctt tgcagttagt aaagggcctc tctttcagaa
     5041 aattgctgat tttccccttt tccttgattg ctgttatttt ttcctggccc ggctttcttt
     5101 atgctgtatc gatacaccaa agaaaaaata atgagggaac gaaggatgcc agttttgcag
     5161 tggggaatgt tatgtgtgct ctcacttctc ctttcaattg gtttcctcgc ggttcatctc
     5221 ccggcggcgc tattacttgg gccgatgatc gccgggatca tctttagtat gcgcggaatt
     5281 actctgcaac ttccccgctc cgcttttctc gccgcccagg ccattcttgg ctgcatgatt
     5341 gcgcaaaacc tgaccggttc gatcctgacc acgctagcgg tgaactggcc gattgtgtta
     5401 gctattttac tggtgacgtt actttccagc gccatcgtgg gttggttatt ggtgcgctat
     5461 agctcactgc ctggaaatac cggtgcctgg ggttcttctc ctggcggcgc ggcggcaatg
     5521 gtcgctatgg cgcaagatta cggcgcagat attcgcctgg tggcgtttat gcaatatctg
     5581 cgagtgctgt tcgtcgcagg ggccgctgtt ctggtcaccc gcatgatgct gggcgataac
     5641 gctgaagcgg ttaaccagca gatcgtctgg tttccgccag tgagcattaa tctcctgctt
     5701 acgattttgc tcgcggtcgt tgccggtaca gtgggatgct tgctacgtct tccttccggc
     5761 acgatgctca tcccgatgct ggcgggcgca gtgctccagt ctggtcagct catcaccatc
     5821 gaactaccgg aatggctgct tgcgatggcg tatatggcaa ttggttggcg gattggtctt
     5881 ggtttcgata aacaaatctt actgcgggca ttgcgcccgc taccgcaaat cctgctgtcg
     5941 atttttgctc tgctggctat ttgtgcgggt atggcgtggg ggctgacccg gtttatgcat
     6001 attgatttta tgaccgccta cctcgccacc aaccccggcg ggcttgatac cgtggcggtc
     6061 atcgccgcag ggagcaatgc cgatatggcg ctcatcatgg cgatgcaaac cctgcgcctg
     6121 ttcagtattt tgctgacggg acctgccatt gcgcggttta tttcaaccta tgcgccgaag
     6181 cgttctgcct aatgctggca gccagggcac cagtaaaacg ggcgagatga cagcgtggtt
     6241 ttctcaatga tgccgccaca gcgttcgcac agttcgccat cacgatgaaa aaccttaaag
     6301 cgaaacagcg ccccatgatg cttattctca tccacctgcc cccgcgtagc gtaggaaaat
     6361 cgaggaatat ccagtaacgc gtgtgcgagt gcatccagtt gcgccgcatt gagatctttc
     6421 gctttatgat ttccagtcaa cccaacctgc cagaggatct ccacccgcaa ataattgcca
     6481 agcccagcca gaaacgcctg atcgagcagt aatccagcaa actgacggtt acgaaagcgc
     6541 ggcgacaata accgttcctt taccacctcc ggcgtcagat tcggatccag cacatcgggg
     6601 ccgactcgtt gtaaaaacgg atgcgtgatc agttgttccg gcgtcaacat ctcaatatcc
     6661 gaggcgctat aaagcagaat agttttgtca accgtttgca gttttacccg caatactcgc
     6721 atagtctgcg gctcttcgcc ggtatcaacc acgcgccaga cgccgtaaag ctgattatgg
     6781 ctgtagagcg ttaagtcgtt ggaaaaatga gttaacaacg ccttaccacg cgtttccaca
     6841 tgggtaacgt gttgaccgat aagttgtgat tgataaggtt ttaactgcgg gaaggcaaac
     6901 cagacatcag ttagtggttt gcctttgatc gccgcctcca ggttatccgc tgcacggcgg
     6961 atctccgggc cttcaggcat ggtgatatcc ttttttcatt ctttattaaa cggggttatg
     7021 ctgcgacaac aatccccttt tcggcaaatg tagagcgtag tcggcgggcg aatgccagtg
     7081 cgtgctcgcc gtcgccatgc aggcagaccg tttgcgccgt gaccgttgcc cattcgccgg
     7141 tgatgctttt gactctgccg tgttgcacca tctccagcgt ttgcgccagc gcctgttctt
     7201 cgttttcaat caacgcgcct ggctggcttc gcggcaccag cgaaccgtct gcctgataac
     7261 cgcgatcggc aaacacttcc tcgcgcgttg tcaggccata ttgcttgcct gcacgaatca
     7321 gctcgcttcc tgccagcccg acgagaatca atgccggatc gcaagcgtat accgctctgg
     7381 cgatggcgtc tgccagttgt gcctctttcg ctgcctggtt gtataacatg ccgtgaggtt
     7441 tgacatgacg cataacaccg ccttgcgcac gggcaatcgt tgccagcgcg ccaatttgat
     7501 acagcgtctg ggcgtaaacg gtttctggcg gcagctgcat gacgctgcgc ccaaaatttt
     7561 ccctgtcggg aaaactcggg tgagcgccaa tcgcaacacc attttttatt gcttcacgca
     7621 cgcaagcctg catggtttgc gcatcgcctg catgaaatcc acaggcaata ttggcagagg
     7681 aaaccagcgt taatagctct gcgtcgctgg catagccttc gcccagatcg gcgttcaggt
     7741 caattttcat tgtgcagccg ccacgctaat tgttcgaaat aacgttgctg atcttgccgt
     7801 gctttcagtg cttcttccag tgaacactgg acaaaatgaa tcggctgacc gagcggaatt
     7861 tgcgccagat ggtacatatc agcctcaatg atacaggcaa tacgcggata accaccggtg
     7921 gtctgtgcgt cgttcatcaa cacaatcggc tgcccgttat gcggcacctg caccacgccc
     7981 ggtaacaaac catgagataa cagttcgcga tcggtggtac gttttaaaat ttgcccctgt
     8041 aagcgatagc ccattcggtt actttgcgag ctaagctgcc agggcgaacg ccagaatgca
     8101 tcctgcgagg cgcgatcgaa ctcatgatat tccggccccg gcaaggcgcg aatgcggttg
     8161 ccccacagca gctgtttaac gccctgcgct tccataaaat cacgcttcga tttgccaatc
     8221 gggagtcggt caccatcctt cagtaaacgg ccttccagcc cgccaatccc cactttgaga
     8281 tcggtgctgc atgaccccat taccggcgga acatcaatac cacccgcgac cgccagataa
     8341 ctgcgcatcc cgtgctgcgg gcgtttaagc gttaaacgct gacctgcttt catcggcaag
     8401 cgccagccgg tccagacggc gttatcatcc agccgcgctt cgcaaccggc ccccgtcaga
     8461 gcaaaccacc catcagtttc gaactcaacc gttaactgac cgagcgtgat ctccagcgcg
     8521 ggggcattgg cgtcattacc caccagtagg ttagcaatgc gcagcgcagg catatccagt
     8581 gcgccgcagt ggctgatacc cgactggcga aaaccgtgac gaccgccatc ctgcacagtg
     8641 gtatacatac ccgcacgaat aatcttcagc atactccctc cttctgcggt acaaagcgca
     8701 cgctgtctcc cggacgtaat aagatgggtt cgtcacgcgc cggatcaaac aggctgagtg
     8761 aggtatgacc aatcaactgc cagccacccg gcgttgccaa cggataaata ccagtctgcg
     8821 gcccgccgat cccgacagaa cctgccggaa cgagcaagcg cggttcagcg cgccgtggcg
     8881 tgtgtagttg ttccggcaaa ctcccgagat acgggaagcc cggttgaaaa cctaaaaacc
     8941 agaccacgta ttccacggag gagtgcaatt caacaacctg tttttcgctc aatccacaat
     9001 gcgccgcgac caccgccaaa tccggtccgg ctgcgccacc gtaaaccacc ggaatttcaa
     9061 taaagcgaga ctccggctcc agcgcctcac tctcctccca ccagcgttgc aaacgctcta
     9121 tggcatccag cgccagcgac tcaggattac gcaaaatcac tgtgatattg ttcatgccag
     9181 gaatggcttc aaccacattc ggcatatcca ccagacgctg tgccagtcgc cagatccgtt
     9241 tctggctagc cagcgtcacc ggcggttcca gctccagcac taccgccgtt tcacctatca
     9301 gataacaacg cgctcgttgc actttcgatc cctctgatta ttcgttatgc aggattagga
     9361 atatcaataa aggtcacatc aagatcggta ttttcattca gccactcgct caatgcgcga
     9421 ataccaccgc gttcagtggc atggtgaccc gcagcataaa aatgcaatcc ctgctcgcgg
     9481 gctgaatgaa tggtctgttc tgaaacttcg ccagtaataa aagcatccac gccaaaacgc
     9541 gcggcgctat cgataaaact ctgcccgccg cccgtgcacc aggcgacgcg ctgaaccacc
     9601 tcaggtccgg tatcgccaca ccataacggc ttacgtccca gacgcgcttc aatccaggaa
     9661 gccagctcca gtcccggcac aggcatggtc agttcgcccc acggcaccaa cggctcaatt
     9721 tcgcccatga ccgtgatccc cagtaacgcc gccagttgtg cgttattacc cagctccgga
     9781 tgcgcgtcca ggggcagatg ccagccatac aggttgatat cattcgccag caacgttttt
     9841 aaacggttac gcttcattcc gcgaatgacc ggagattcac ctttccagaa gtagccatga
     9901 tgcacaatga ccgcatccgc gcccagacgc accgcttcat cgagcaaagc ctggctggcg
     9961 gtgacaccgg taacaatttt ttgcaccgtc tctttgcctt ccacctgcaa accattcggc
    10021 gcgtaatcac taatcgccgc gctgttcagt ttttcgttaa tcagttgttc cagttcggtg
    10081 tttttcatct tcgcgccttc cgtattctga tagctacacc gttaacatag cgttacctcg
    10141 cctgttcgtc gatagccaat cttttgacat attaagaaac aaacccgacg ccaaaagacg
    10201 caaattgttt atatcatcac gcttcagcta atgtatattt atgcaatcag acagcatatt
    10261 gcataaataa tcagatctgt atcaaagttt taactttaag gaaacacaag cacgccgcgc
    10321 aactattacg tgacgttgca gtcaattgtg gaattattat gaataaacac gcatcacaac
    10381 cgcgcgctat ttactatgtc gttgcgctgc aaatctggga atattttagc ttttacggca
    10441 tgcgtgccct gctgatcctc tatctcacca atcaactaaa atatagcgat aatcacgcct
    10501 acgagttatt tagcgcctac tgttcgctgg tatatgtcac gccaatcctc ggtggctttt
    10561 tggcggataa agttctcggc aatcgcatgg cggtgatgct gggtgcgttg ttgatggcga
    10621 tcggtcatgt ggtgctgggt gccagtgaga tccatccgtc attcctctat ctgtccctgg
    10681 cgattatcgt ttgtggctat ggtctgttta aatccaacgt cagttgcctg ctcggcgagc
    10741 tgtatgagcc aaccgatccg cgtcgtgatg gcggtttctc gctgatgtat gcggcgggta
    10801 acgtggggtc tattatcgca cctatcgcct gtggctacgc ccaggaagag tacagttggg
    10861 cgatgggctt tggcctggcg gcggttggca tgatcgcggg tctggtcatt ttcttatgtg
    10921 gcaatcgtca tttctctcat acccgcggcg ttaacaaaaa agtactgcgt gcgacaaact
    10981 ttctcctgcc caactgggga tggctgctgg ttctgctggt ggcaacacct gcgctgatca
    11041 ccgtactgtt ctggaaagag tggtcggtat acgccttgat tgtcgcgact attattggcc
    11101 tgggtgtatt ggcaaaaatt tatcgcaaag cagaaaacca gaaacagcgt aaggagctgg
    11161 ggctgattgt taccctcacc ttcttcagca tgttgttctg ggcattcgca caacaggggg
    11221 gtagctcgat tagcctttat atcgaccgct tcgttaaccg cgatatattt ggttataccg
    11281 ttccgactgc tatgttccag tcgattaatg ccttcgcagt tatgctgtgc ggtgtgttcc
    11341 tggcgtgggt ggttaaagaa agcgtcgcag gtaatcgtac cgtgcgcatc tgggggaaat
    11401 ttgctcttgg tctcggcctg atgagcgccg gattctgcat tctgacctta agcgcccgct
    11461 ggtctgcaat gtatggtcac tcttctctgc cactgatggt gttaggcctg gcggtgatgg
    11521 ggtttgcgga actgtttatc gacccggttg ctatgtcgca gattacgcgc attgaaatcc
    11581 ccggcgtgac cggcgtatta accggcattt atatgctgct ttccggcgcg attgcgaact
    11641 atctggccgg cgtgattgcc gatcagacat cgcaggcttc gtttgatgct tccggagcga
    11701 tcaactactc catcaatgca tatattgaag tatttgatca aattacctgg ggcgcactgg
    11761 cgtgtgtagg actggtgctg atgatttggc tgtatcaggc gcttaagttc agaaaccgcg
    11821 cgctggcgct ggaatcttaa caggccagca ccgatgcctg ataagacgct cgctgcgttt
    11881 tatcaggctc ctttcctcgc ttcttcgtac gctgccagcg ttcgcagcct tgcctctttg
    11941 tgatcaacta tcggttgcgg ataatccagc gtcacacctg ctttctgcgc ccacttccac
    12001 ggctcatgca ccactttccc tggcacatcg cgcagttccg gtagccactg gcggataaac
    12061 tcgccctcac ggtcaaattt ctcgccctgg gttgtcgggt taaaaatacg aaaatacggc
    12121 gctgcatcgg ttccggttga agcggcccac tgccagccac cgttattggc tgccaaatca
    12181 ccatcaatca gctgcgacat gaaatatcgc tcaccttcgc gccagtcgat caataaatct
    12241 ttcaccaaaa aactggctgt aatcatccgt agccggttat gcatccagcc ggtgctgtta
    12301 agctgacgca tggcagcatc aacaatcggg tatcccgttt tgccttcctg ccaggccttt
    12361 aaatgcgcgg aattgctctg ccactgcacg cggtcggtcc agacaataaa cggacgatgt
    12421 ttacacagcg aggggtaata cgttatcaga tgacggtaaa actcgcgcca gatcagctca
    12481 ttaagccaga tactaccggc cccaccgtcc agcgcctgcg gctgttccgc caacaggcga
    12541 tgcaaacact ggcgaggcga taacccgccc gttgccaggc tggcggacaa acggctggtg
    12601 ccttctaccg cagggaaatc tcgctgttgc tcatactctc cggcaccgtt ctggcaaaac
    12661 tggcgtaatt gcgcaatcgc cgctttctct tccaccggga aatgcgcagt atcgaaaggc
    12721 tgacgaggat aattcagcgt aatggatggc gcgggatcta tcgatccgct actacgtact
    12781 tttggcgcag cgacgcactc cggcatccct tctcgcagcc gtttaagcca ggcgttttta
    12841 aatggcgtaa agactttgta catctcatga ttgccagtca tcaccgcccc cggcggcagg
    12901 atcacgccgt cgtcaaatcc ttcacacacc acgttacgca gcgccctctc agcctgcaca
    12961 tcccgcgccc gctcattcac ctcatactga tagttataaa acagatgggt aacacgattt
    13021 tccgcgcaca cctgtttaac tatttcgacg ctggcggcaa agtcatccac ttcatgaaac
    13081 agcaaaggaa tgcctttttc cgccagcgcg atttgtagcc cgttcagttg tgcattgata
    13141 aattcagcct gacgcggcga catattatgc gccgcccact ggcgtggtgt agcgatatac
    13201 aacgccagca cgcttgcaga cgaattgcgg caggcggcag ccagcgcgag attatcatgc
    13261 tgacgtaaat cctggcgaaa ccagaccaga tgggtagtca taaaactcct gaatggcgca
    13321 agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag gcgtcaggat
    13381 aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagcggttgc tcgccaccgc
    13441 gataactgtc gataagcgcc gccagctgct ggcgttctgt ggaatcaata tgagggcgaa
    13501 aataaccctg aacgtgcatc aaaacattgg tgtggtcacg tcggttggcg gggtgagaaa
    13561 gcaaaacaac tacgcgttgg cggtactggt tgtaatagtc atccagattt tgccactggt
    13621 gaatatcagc cacgaacggg ccaagtttgc ggtactcggg ctgggaatgg gccagaaaga
    13681 cgagtttata gcggctgtga taatcgttga gtgccccgcg cgttaagcca tgttctttca
    13741 gtcgatgtaa ctcataaagc gcgaagatac ggattagagt gctttcatca aatcgttgct
    13801 gattcattac tgctcctgag atattaacag gagtaagtgt agacgttggc aaatgatgtt
    13861 tgtgaagtta atcctgttgt agcagggcaa aattacgaaa gcacgctccc cgcaaggact
    13921 gacgccagat attttctgtc catggctgcc ctttccattc aaacgtgaaa tcaataagtt
    13981 catcagggac atcgcaaaca atatgaagac ggatttcttc tctgccgtga ctcttttcac
    14041 tcattgcgta actgccatgc tctggattat ttaattcttt cagcggaaat ttttcctcga
    14101 aggctttatt tagccgcccc tggtttcctt ttacagcgaa taaataatca ccgccctgtt
    14161 tttgtatctt ctctgcaata tctttctggc aacccatc
//
LOCUS       DAZUCC010000128        14079 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZUCC010000128 DAZUCC010000000
VERSION     DAZUCC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.129"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(205..281)
                     /locus_tag="ACHY09_003274"
     tRNA            complement(205..281)
                     /locus_tag="ACHY09_003274"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:Arg,seq:acg)
     gene            complement(285..377)
                     /locus_tag="ACHY09_003275"
     tRNA            complement(285..377)
                     /locus_tag="ACHY09_003275"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:Ser,seq:gct)
     gene            complement(693..878)
                     /gene="csrA"
                     /locus_tag="ACHY09_003276"
     CDS             complement(693..878)
                     /gene="csrA"
                     /locus_tag="ACHY09_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFS3886209.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1113..3743)
                     /gene="alaS"
                     /locus_tag="ACHY09_003277"
     CDS             complement(1113..3743)
                     /gene="alaS"
                     /locus_tag="ACHY09_003277"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS3886210.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVNSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLNEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3871..4371)
                     /gene="recX"
                     /locus_tag="ACHY09_003278"
     CDS             complement(3871..4371)
                     /gene="recX"
                     /locus_tag="ACHY09_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS3886211.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4440..5501)
                     /gene="recA"
                     /locus_tag="ACHY09_003279"
     CDS             complement(4440..5501)
                     /gene="recA"
                     /locus_tag="ACHY09_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS3886212.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5581..6078)
                     /gene="pncC"
                     /locus_tag="ACHY09_003280"
     CDS             complement(5581..6078)
                     /gene="pncC"
                     /locus_tag="ACHY09_003280"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFS3886213.1"
                     /translation="MTDSELMQLSEQVGQSLKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6223..7308)
                     /gene="mltB"
                     /locus_tag="ACHY09_003281"
     CDS             complement(6223..7308)
                     /gene="mltB"
                     /locus_tag="ACHY09_003281"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFS3886214.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7564..8127
                     /gene="srlA"
                     /locus_tag="ACHY09_003282"
                     /gene_synonym="gutA"
     CDS             7564..8127
                     /gene="srlA"
                     /locus_tag="ACHY09_003282"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS3886215.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8124..9083
                     /gene="srlE"
                     /locus_tag="ACHY09_003283"
                     /gene_synonym="gutE"
     CDS             8124..9083
                     /gene="srlE"
                     /locus_tag="ACHY09_003283"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS3886216.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9094..9465
                     /gene="srlB"
                     /locus_tag="ACHY09_003284"
                     /gene_synonym="gutB"
     CDS             9094..9465
                     /gene="srlB"
                     /locus_tag="ACHY09_003284"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS3886217.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9469..10248
                     /gene="srlD"
                     /locus_tag="ACHY09_003285"
     CDS             9469..10248
                     /gene="srlD"
                     /locus_tag="ACHY09_003285"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFS3886218.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10354..10713
                     /gene="gutM"
                     /locus_tag="ACHY09_003286"
     CDS             10354..10713
                     /gene="gutM"
                     /locus_tag="ACHY09_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS3886219.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMYAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10780..11553
                     /gene="srlR"
                     /locus_tag="ACHY09_003287"
                     /gene_synonym="gutR"
     CDS             10780..11553
                     /gene="srlR"
                     /locus_tag="ACHY09_003287"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFS3886220.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            11546..12511
                     /gene="gutQ"
                     /locus_tag="ACHY09_003288"
     CDS             11546..12511
                     /gene="gutQ"
                     /locus_tag="ACHY09_003288"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFS3886221.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12508..14022)
                     /gene="norR"
                     /locus_tag="ACHY09_003289"
     CDS             complement(12508..14022)
                     /gene="norR"
                     /locus_tag="ACHY09_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFS3886222.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     IVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
BASE COUNT         3275 a         3900 c         3507 g         3397 t
ORIGIN      
        1 ggatgcatcg ggaaacttac tttactgcag attttttgat accgctacta aagccgtatc
       61 aagtaagaga tggtgcatcc gggaggatta ctcgccttgc ggctcgccct tcgggccgtt
      121 gctaaagcaa cgttatcctc cctggtgcct gcggttatta ccgccacctc ttccatacct
      181 tcatcgctga aggtacgtag aatatggtgc atccgggagg attcgaacct ccgaccgctc
      241 ggttcgtagc cgagtactct atccagctga gctacggatg caaatggcgg tgaggcgggg
      301 attcgaaccc cggatgcagc ttttgaccgc atactccctt agcaggggag cgccttcagc
      361 ctctcggcca cctcaccaca cgcctcttac gagtgcttcg aagaacttgt ttatcgctca
      421 tcgtcgctgc gtggcgcaca tattactttc tgagacttat aagtcaaaca atttttccca
      481 cacttttatc gtttgcacac ttcacgctca attagtctgt aaaaacgtca aaaagagtgt
      541 tttatcaaca gaaaaacgga ggtctgtcag atagcagtaa tgcaaaaaaa tggagactta
      601 agttgaatga acgggagtaa agcgaaaaga caatggagtg aagaagaaat tttgagggtg
      661 cgtctcaccg ataaagatga gacgcggaaa gattagtaac tggactgctg ggatttttca
      721 gcctggatac gctggtagat ctcttcacgg tgaacagaaa cttccttcgg ggcatttacg
      781 ccaatacgta cctggttgcc ctttacccct aaaactgtca cggtgacctc atccccaatc
      841 atgagggtct caccaactcg acgagtcaga atcagcattc tttgctcctt gaaagattaa
      901 aagagtcggg tctctctgta tcccggcatt atccatcata taacgccaaa aagtaagcaa
      961 tgacaaacac attacatcta agcagtcatg gcattacatt ctgttaaacc taagtttagc
     1021 cgatatacac aacttcaacc tgactttatc gttgtcgata gcgttaatgc gaatgccgtg
     1081 aagcgagtcc acggcattgc ctgacgctta tattattgca atttcgcgct gacccagcct
     1141 ttcacactgg ctaacgctgc aggtaaggcc gcagcatccg taccaccggc ttgcgccatg
     1201 tcaggacgtc caccaccctt gccgcccacc tgctgagcga ccataccaat cagttcccct
     1261 gctttcacac gatctgtgac gtccttagat acgcctgcaa tcagagaaac cttaccttcg
     1321 gctaccgttg ccagcacgat aattgtcgac cccagctgat tttttaaatc gtcaaccatg
     1381 gtacgcaaca ttttcggctc aacaccgcta agctcgctaa ccaacagctt aacaccatta
     1441 acatcaattg ccttactgga aagatttgcg ctctcctgtg cggcagcttg ttctttaagc
     1501 tgttgtaact ctttttccag ctgacgcgta cgttccagta ctgagcgcac tttatcagcc
     1561 agattattgc tatcgccttt cagcagatgc gcgacttcgt ttaagcgatc gctgtctgca
     1621 tgaacggtgg cgatagcgcc ttctccggtt accgcttcga tacgacgaac gcctgcagca
     1681 gtacccgatt cagagatgat gcggaacaga ccaatatcac cagtgcgact ggcgtgagta
     1741 ccgccacaca gctcggtgga gaaatcgccc atgctcagca cacgtacacg ttcatcatac
     1801 ttctcgccga acagcgccat cgcacctttc gctttcgccg cttcgagatc catgatgttg
     1861 gtttcaatcg gcaagttacg gcgaatctgt gcgttcacca ggtcttcgac cgcacgaatc
     1921 tcttctggtt tcatcgcttc gttgtgtgag aagtcgaagc gcagcacttt gtcgttaacc
     1981 agtgaacctt tctgcgatac atgagtaccc agaacctggc gcagcgcagc gtgcatcagg
     2041 tgcgttgcgg agtgattcag acgaatacgg gcgcgacgag cttcatcaac atcagcctgc
     2101 accgcgtcgc ccactttcag agaacccgca gcaagtttac cgatgtgacc aatcgcctgg
     2161 ccgtatttct gcgtatcttc caccgcaaag gagaagttag cgcctttcag ttcgccttta
     2221 tcaccaacct gaccgccgga ttccgcatag aatggcgttt gatccagcac gaccacagct
     2281 tcctggcctg cattgatggc atcaaccgct ttaccatcaa caaacagcgc ggtcactttg
     2341 ccgttcagtt ccagatggtc atagccttta aattcagatg cactgttaac acggatcatt
     2401 gcgttgtaat cggcaccaaa gccgctggct tcgcgcgcac gacgacgctg ctcttccatt
     2461 gctgcttcaa atccagcttc gtcaactttg atgttgcgct cacgacaaac atcagccgtc
     2521 aggtcaaccg ggaagccata ggtgtcgtac agacggaaag ccgtttcacc atccagcgta
     2581 tcaccagaaa gttttgccag ctcttcgtcc agcagcgcca gaccgcgctc cagagtacgg
     2641 gcaaactgct cttcttcagt cttcagcacc tgctcaacct gcgcctgctg gcgtttcagg
     2701 tcttcacccg cagagcccat tacgtcgatc agcggaccaa ccagtttgta gaagaaggtt
     2761 tctttcgcgc ccagcatatt gccgtgacgc actgcgcgac gaatgatacg acgcagtaca
     2821 taaccacggt tttcgttgga cggcattacg ccatccgcga tcaggaacgc acaagaacga
     2881 atgtggtcag cgattacgcg cagcgatttg ttgctcaaat cggtcgcgcc agtgactttc
     2941 gctaccgcct ggatcagcgt acggaacagg tcgatgtcat agttagagtt aacgtgttgc
     3001 agcaccgcag caatacgctc cagacccata ccggtatcta cagacggctt cggcagcggt
     3061 tccatcgtgc catctgcctg gcggttgaac tgcatgaaga cgatgttcca gatctcaatg
     3121 tagcggtcgc cgtcttcttc cggacttccc ggagggccac cccaaatgtg gtcgccgtga
     3181 tcgtagaaga tttcggtgca cgggccgcac ggaccagtgt cacccatctg ccagaagttg
     3241 tcagatgcgt atggcgcacc tttgttatcg ccgatgcgaa taatacgttc gcgcgggatc
     3301 ccgacttctt tttcccagat ttcgtaggct tcgtcgtcgc tttcatagac ggtaacccac
     3361 agacgctctt tcggcagggc aaaccatttt tcgctggtca gcagttccca tgcaaactga
     3421 atggcatcgt gtttgaaata gtcgccgaag ctgaagttgc ccagcatttc gaagaaggta
     3481 tggtgacgcg cggtgtaacc gacgttttcc aggtcgttgt gtttaccacc cgcacgcacg
     3541 cagcgttggg aagtggtagc gcgggaataa ttacgcttgt cgagcccaag gaacacatcc
     3601 ttgaactggt tcatcccggc gttggtaaac aacaaagttg ggtcgttatg gggtaccagg
     3661 gagctgctgg caactacctg atgtccctta ctatggaaaa agtcgagaaa cgcctgacgg
     3721 atctcagcgg tgctcttgct cataattatc ctgaaatcaa gctaacgaaa tatcgccacc
     3781 agctacagcg tgttttaacc gccgggctgg taactgaaaa gtgggaataa gataagtttt
     3841 cttgactggg aagtaaaatc ccgtatgcgt tcagtcggca aaatttcgcc aaatatcctg
     3901 gatatcttcc atcagatagc cacgatagag cagaaaacgc tggatcttaa ctttttctga
     3961 aaagacagtt ggcaaaggtt cgccatattt tcgcgtcgcc tgatcgcgcg ccagtgcgca
     4021 ccagtcgatg tcacattcac gcatcgcttt ttctgtcgct tcgcgggaaa tacctttctg
     4081 attcagttcc tgacgaatac gcgcaggtcc ataacctttg cggctacggc tggcgataaa
     4141 gcgcgcaaca aatcggctgt catcgagata gccatgttca tggcaccagg cgataacgcg
     4201 ctcgtaatct tctgccgtgg catcaatctc ttccgggcca tttttgccca tgatcggtgc
     4261 cgcgagttta cgtcgcaatt cttgctcact gtgatcgcgc accgccagaa tgcgtaccgc
     4321 acgatccaac aggcgagcat atgccgggcg acgggacgtt gattctgtca tggcatatcc
     4381 ttacaactta aaaaagcaaa agggccgcag atgcgaccct tgtgtatcaa acaagacgat
     4441 taaaaatctt cgttagtttc tgctacgcct tcgctgtcat ctacagagaa atccggcgtt
     4501 gagttcgggt tgctcagcag caactcacgt actttcttct cgatctcttt cgcggtttcc
     4561 gggttatctt tcagccaggc agttgcattc gctttacctt gaccgatctt ctcacctttg
     4621 tagctgtacc acgcgcctgc tttctcgatc agcttctctt tcacgcccag gtcaaccagt
     4681 tcgccgtaga agttgatacc ttcgccgtag aggatctgga attcagcctg tttaaacggc
     4741 gcagcgattt tgttcttcac cactttcaca cgggtttcgc tccccaccac gttttcgccc
     4801 tctttcaccg cgccgatacg acggatgtcg agacgaacag aggcgtagaa tttcagcgcg
     4861 ttaccaccgg tagtggtttc cgggttaccg aacattacac caattttcat acggatctgg
     4921 ttgatgaaga tcagcagcgt gttggactgc ttcaggttac ccgccagctt acgcatcgcc
     4981 tggctcatca tacgtgccgc aaggcccatg tgagagtcgc cgatttcgcc ttcgatttcc
     5041 gctttcggcg tcagtgccgc cacggagtca acgacgataa cgtctactgc gccagaacgc
     5101 gccagggcgt cacagatttc cagtgcctgc tcgccggtat ccggctggga gcacagcagg
     5161 ttgtcgatat caacgcccag tttacgtgcg tagattgggt ccagcgcgtg ttcagcatcg
     5221 ataaacgcac aggttttacc ttcgcgctgc gctgcggcga tcacctgcaa cgtcagcgtg
     5281 gttttaccgg aagattctgg tccgtagatt tcgacgatac ggcccatcgg cagaccacct
     5341 gccccaagcg cgatatccag tgaaagcgaa ccggtagaga tggtttccac atccatggaa
     5401 cggtcttcac ccaggcgcat gatggagcct ttaccaaatt gtttctcaat ctggcccagt
     5461 gctgccgcca acgctttctg tttgttttcg tcgatagcca tttttactcc tgtcatgccg
     5521 ggtaataccg gatagtcaat atgttctgtt gaagcaatta tactgtatgc tcatacagta
     5581 tcaagtgttt tgtagaaatt gttgccacaa ggtctgcaat gcatacgcag tagcctgacg
     5641 acgcaccgca tcacggtcac cgctgaaaca ttcccgccgg gtaatgcctt caccacgggc
     5701 agtggcaaaa gcaaaccaga cggtgccgac aggtttctct tcactgccgc catccggccc
     5761 ggcgatacca ctaatagaca cggcgtaatc agcacgagcc gctttcagtg cgcctatcac
     5821 catttccacc acgacaggtt cgctcaccgc gccatgctgt gccagcgtct cttcgcgtac
     5881 accgatcatt tgcgcttttg cttcgttact gtaggtgaca aatccgcgtt caaaccaggc
     5941 ggaactgccg gcaatatcgg taatcacttt cgctacccaa ccgccggtac aagactcagc
     6001 agttgttacg gttgcgccac gggctttcag cgactgccca acctgttcgc ttaactgcat
     6061 cagttcactg tcagtcatca caacctctgt cagtcaaaaa tttatgccgg acaagataac
     6121 actttcgtca ggaagatggt gttggcgttg caattttacg aggaggattc agaaaaaagc
     6181 tgattagcca gagggaagct cacgcccccc tcttacaaat agttactgta cgcgcgccag
     6241 cgccacggct tgtcctaact gccagaccgc cattgcgtaa tgggtgctgt ggttataacg
     6301 ggtaatggtg tagaagttcg gcagaccgta ccagtactgg tagccggtgc caacatccag
     6361 acgcagcagg ctggcttgtt gatggttgcc cagcggctgc tgtggcgtta aacctgccgc
     6421 ggcaagctgc gaaatgctgt acttggtttt gaagccattt ggcaagcctg gagcctgacc
     6481 gtttgccatc accgcaacct gatcaccttt cacccagccg tgcgctttga aatagttcgc
     6541 cacactacca atggcatcaa ccggatccca caggttgata tgcccgtcgc cgctgaaatc
     6601 taccgcatat tgtttgtaag acgacggcat aaactgtccg tagcccatcg ccccggcaaa
     6661 ggaacctttc agattgagcg gatcgtcctg ctcgtcgcgc gccatcagca ggaaggtttc
     6721 cagctcgcca gaaaaatact ccgcgcggcg tgggtagtta aatgacagcg ttgccagcgc
     6781 atcgaggatg cgagttttcc ccatcacgcg cccccagcgg gtttcaacgc cgataatccc
     6841 gacgataatt tccggcggta caccatacac ctgccacgcg cgattcaacg catcttcata
     6901 ctgattccag aaaaccacac cgttctgcac gttgtccggc gtaataaatt ttttgcgata
     6961 acgcagccat gcgccgttcg gacctgatgg cggtttcacc gatgtggttg gtgcctggtt
     7021 atccatcagc cgcagtaccg aatccagacg tttcgcctgg gagagaattt cctgcaactg
     7081 ctgacgatcg aaaccgtgtt tgttcaccat tttgtcgatg aactgctggg cattcggatt
     7141 attggcgaag tcaccgccca tctgcatcac attgtgctgc ggctcaagca ggaagccgcc
     7201 agacggcgtt ccggtggtcg tttcagtctc agtaggtttt ggcttgctgc tacaggcggc
     7261 aagcaacaca aaaaggggaa gcaatgttac ataacgacgc ttgaacatga ggggtccatt
     7321 taacagattc aaccaggggc aagtatggta aagcatcacg ccccgcacaa ggaagcggta
     7381 gtcactgccc gatacggact ttacataact caactcattc tcctcgctat ccttttattc
     7441 aaactttcaa attaaaatat ttatctttca ttttgcgatc aaaataacac ttttaaatct
     7501 ttcaatctga ttagattagg ttgccgtttg gtaataaaac aataaatcct gaaggagaga
     7561 acaatgatag aaaccattac tcatggtgca gagtggttta tcgggctgtt ccaaaagggc
     7621 ggagaggtgt ttaccgggat ggtgaccggc attcttccgc tgttgattag tctgctggtt
     7681 atcatgaacg cactgattaa ttttatcggt cagcatcgta ttgaacgttt tgctcaacgt
     7741 tgcgccggta accctgtttc ccgttacctg ctgttaccgt gcattggcac gtttgtcttt
     7801 tgcaatccga tgaccttaag cctgggtcgc tttatgccgg aaaagtacaa acccagctac
     7861 tacgcggccg cctcttatag ctgccactca atgaatggcc tcttccccca tatcaaccca
     7921 ggcgaactgt ttgtttatct tggcattgcc agcggtctga caacactgaa cctgccactt
     7981 ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaacttctt ccgcggctgg
     8041 gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact tgaacaaaaa
     8101 gttcatctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga acgggtggct
     8161 ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat atcaccgccg
     8221 gtacccggcc tgcgattgtt gacaaactgg cacagcttac tggctggcag gctattgacg
     8281 gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc gactgtggcg
     8341 gcacattacg ctgcggcatt tatccgaaac ggcgtattcc caccattaat atccactcga
     8401 cgggcaagtc cggtccacta gcgcaataca ttgtagaaga tatttatgtc tctggcgtaa
     8461 aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc gtgggccgtg
     8521 actatgacac cagcaagaaa atcaccgaac aaagcgatgg tttactggcg aaggtgggaa
     8581 tgggtatggg ttctgccgtt gccgtgttgt ttcaatctgg tcgtgacacc atcgacactg
     8641 tattaaaaac cattctgccg tttatggcat tcgtttcggc gcttatcggc atcattatgg
     8701 cttccggtct tggcgactgg attgcccacg gtcttgctcc gctggcgagc catccactgg
     8761 gtctggtcat gctggcgctc atctgctcct tcccgctgct ttcacctttc ctcggcccag
     8821 gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt ctcggcaata
     8881 ttccgccgca tctggcttta cctgcactgt ttgccatcaa cgcgcaggcg gcctgcgact
     8941 tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc gtcggtgtcc
     9001 cttctgttct ggtgagccgc tttttaaccg gtgcgccaac tgtactgatc gcctggtttg
     9061 tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca gaccaccatc
     9121 acccgtattg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat cacctttcgt
     9181 gaaggcgcgc cagcggatct cgaagagtat tgcttcattc attgccacgg cgagttgaaa
     9241 ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc ggtgaccgcc
     9301 gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct gcgcttcgat
     9361 ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt acccgacgat
     9421 atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat gaatcaggtt
     9481 gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg tctggctgcc
     9541 gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa tgtggcacaa
     9601 gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga cgccactagc
     9661 gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg cgtggatttg
     9721 ctggtctaca gcgccggaat agcaaaagca gcctttatca gcgacttcca gctcggcgat
     9781 tttgaccgtt cgctacaggt gaatctggtg ggttatttcc tgtgtgcccg tgaattttcg
     9841 cgtttgatga tccgcgacgg gattcagggg cgcattattc agatcaactc gaaatccggc
     9901 aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg cgtcgggctg
     9961 actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc actgatgctc
    10021 ggtaatctgc tgaaatcgcc gatgtttcag tcgctattgc cacaatatgc gaccaagctg
    10081 ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct caaacgcggc
    10141 tgcgattatc aggatgtgct gaatatgctg ctgttctacg ccagtcctaa ggcgtcgtac
    10201 tgcactggtc agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatt aacagcggag
    10261 atccgttaag gatctccgcg agactaataa aatgcctgat gcgctacgct catcaggcat
    10321 acaggacttc cgccactaca ttaaggaaaa gttatggtat ccgcactcat caccgtcgcc
    10381 gttatcgcct ggtgtgcgca actggcctta ggcggttggc aaatttctcg ttttaaccgt
    10441 gccttcgaca cactatgcca gcaagggcgg gttggcgtgg gccgttccag cgggcgcttt
    10501 aaaccgcggg tcgtggtcgc catcgccctg gacgatcagc aacgcatcgt cgacaccttg
    10561 tttatgaaag gactgaccgt cttcgcccga ccacaaaaaa ttcccgcaat taccggtatg
    10621 tatgcgggtg atttacagcc cgatgtgatc tttccccatg atccactatc acagaatgct
    10681 ctatcattgg cgcttaaact gaaacgtgga taatttcgtt gtgaatgtta cttgcttgcg
    10741 aagttatcat tttgaaacct aaatcaggta atcacgccca tgaaacctcg tcagcgtcag
    10801 gccgccattc tggagtatct gcaaaagcag ggtaaatgct cggttgaaga attggcgcaa
    10861 tactttgaca ccacaggcac aaccattcgc aaagatctgg tcattctgga acatgccgga
    10921 accgtcattc gtacttatgg cggagtggtg ttgaataaag aggaatccga tccgccaatc
    10981 gatcataaaa cactcatcaa cacccacaag aaagagctga ttgcagaagc tgccgttagt
    11041 tttatccatg atggcgattc gatcattctt gatgctggca gtaccgtttt gcagatggtc
    11101 cccctgctct cgcgctttaa taacatcaca gtgatgacca acagcctgca tatcgtcaat
    11161 gcgctatccg aactggataa cgaacaaact atcctgatgc caggcggaac atttcgtaaa
    11221 aaatcggcct catttcacgg gcaactggca gagaatgcct tcgagcattt caccttcgat
    11281 aaattgttta tgggcaccga cggcatcgat ctcaatgcgg gcgtaaccac gttcaacgag
    11341 gtttataccg tcagtaaggc aatgtgcaat gccgcgcgcg aagtgatttt gatggcggac
    11401 tcatcaaagt ttggccgtaa aagccccaac gtagtttgca gtcttgaaag cgtcgataag
    11461 ctaattaccg acgcaggtat cgatccagcg tttcgtcagg cgctggaaaa gaaagggatc
    11521 gacgtgatca taaccggaga gagcaatgag tgaagcacta ctgaacgcgg gacgtcagac
    11581 gttaatgctg gagttgcagg aagcaagccg tttaccggaa cgtctgggcg atgattttgt
    11641 tcgcgccgcc aatatcatcc tgcactgcga aggcaaagtg gtggtttcgg gaattggcaa
    11701 atcgggccac attggtaaga aaatcgccgc aacgcttgcc agcaccggca ctcctgcctt
    11761 ttttgtccat ccggcagagg cattgcacgg cgacctgggg atgatcgaaa gccgcgatgt
    11821 gatgctgttt atctcttact ccggtggcgc gaaggaactg gatctgatta ttccgcgtct
    11881 ggaagataaa tctatcgcgc tgctggcgat gaccggcaaa ccgacgtcac cgctgggcct
    11941 ggcggcgaaa gcggtgctgg atatctccgt agaacgcgaa gcctgcccga tgcaccttgc
    12001 gccgacctcc agcaccgtca ataccctgat gatgggtgac gcgctggcga tggcggtcat
    12061 gcaggcgcgc ggatttaatg aagaagattt tgcccgctcc caccctgctg gagcactggg
    12121 cgctcgcttg ctgaataaag tgcatcatct gatgcgccgt gacgatgcca tcccgcaggt
    12181 ggcgttaacc gccagcgtga tggacgcgat gctggaactt agccgcaccg gtttaggtct
    12241 ggtggcagtg tgcgatgatc aacgactggt gaaaggcgtc tttaccgacg gtgatttacg
    12301 tcgctggctg gttggcggcg gcgcactcac cacgccagtc aatgaagcga tgacggtcgg
    12361 cggcaccacg ttgcaatcgc aaagccgtgc catcgacgcc aaagaaatcc tgatgaaacg
    12421 caaaatcacc gccgcaccgg tagtggatga aaacggcaaa ctcaccggcg caattaacct
    12481 gcaggatttc tatcaggccg ggattattta atccttcagc cccagacgtt tcgccagccg
    12541 atgcaggttg gcgacgtcgg tttccagcat ccgcgcacag gcggcccagt tgtgatgatt
    12601 ttgcgccagt gcctgacgaa tggtttcacg ctggaacgct tctgtcgctt cacgcaggtt
    12661 ttgcttaacg ataggcaccg ccgccgcttc tggcggcggc aacgtcacct caggaaaagc
    12721 aaaatgttgc gcctcaagaa tcacttcatc gccgctgcga gtggctctcg acagtactac
    12781 cgcccgatga atagcatgtt ccagttcgcg cacgtttccc ggaaaactgt agtgttgcag
    12841 taaatttcgc gccccggcac ttaataccac gcgggagagc cccagccgca aacgacactg
    12901 ctcgcagaaa taccccgcca gcagaatgac atcatcgccc cgctcacgca gcggcggcac
    12961 cgaaagtgga aacacgctca ggcgatgaaa caaatcggcg cggaatcgcc ctgccagcac
    13021 ctcttcgcgt aaatcgcggt tagtcgccgc cagcacgcgc acatcgaccc gcaaactgcg
    13081 gtcatcgcca acgcgctgaa tatcgccata ctgcaacacc ctcagcagct tggcctgcaa
    13141 tgccaacgac aactcgccga tctcatcgag aaacagcgtg ccgttatccg ccatttcaaa
    13201 cttcccgctg cggttactga tagcaccagt aaacgcccct ttcacatgcc cgaacaactc
    13261 actttccgcc acactttccg gcagtgcggc acagttgaga tagaccagcg gattcaccgc
    13321 ccgtggcgag gcttcatgaa tcgctttcgc caccagctcc ttaccggttc ccgtctcacc
    13381 gctaattaaa acgttgagat cggatgccgc cacaatctca atctcttttt tcaattgcgt
    13441 catgcctggg gacaaaccaa tcatctgcgt ctgtttcacc gcttcaaacg gcgcggcatc
    13501 gcctggcagc atattctggc tttccagttg ttcaatcagt aacgcattac ttaacgctcc
    13561 cgccgccagt gcggcaatca gccgtagctc ttcgtcgctg aaaacatcga actgatcggg
    13621 ctgcatcccg tcgagcgtca aggcaccgat caggttttgc ccggcaaaca acggcaaacc
    13681 aacgcaggcg tgaaccttca gactctcctg ccctggaatc aaaccgtcat aaggatcggg
    13741 caattcgctg tctgcgggaa agcgcaccac atcacccgcg cgggcaatcg cttccagccg
    13801 tggatgacct tccagcgcaa agcgtctacc gagtacatcc ttcgccagcc cgtcgatggc
    13861 aagcggaata aactgccgcg agtcgtaacg tagcaacgca gacgcatcgc actccagcac
    13921 ctgacgcagc gtggtgatca ggcgctgaaa acgatcctga tgaccaatcc cacgctgcaa
    13981 ttcgatggcg atattcgcca gcacatcaac ggaaaaactc atctttgcct cactgtcaat
    14041 ttgactatag atattgtcat atcgaccatt ttattgata
//
LOCUS       DAZUCC010000129        14054 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZUCC010000129 DAZUCC010000000
VERSION     DAZUCC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.130"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..279)
                     /gene="yddM"
                     /locus_tag="ACHY09_003290"
     CDS             complement(<1..279)
                     /gene="yddM"
                     /locus_tag="ACHY09_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFS3886223.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVT"
     gene            complement(279..557)
                     /locus_tag="ACHY09_003291"
     CDS             complement(279..557)
                     /locus_tag="ACHY09_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3886224.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            complement(743..1753)
                     /gene="adhP"
                     /locus_tag="ACHY09_003292"
     CDS             complement(743..1753)
                     /gene="adhP"
                     /locus_tag="ACHY09_003292"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFS3886225.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            complement(1887..3584)
                     /gene="maeA"
                     /locus_tag="ACHY09_003293"
     CDS             complement(1887..3584)
                     /gene="maeA"
                     /locus_tag="ACHY09_003293"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFS3886226.1"
                     /translation="MEPKTKRQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            complement(3741..3878)
                     /gene="sra"
                     /locus_tag="ACHY09_003294"
     CDS             complement(3741..3878)
                     /gene="sra"
                     /locus_tag="ACHY09_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFS3886227.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(3980..4195)
                     /gene="bdm"
                     /locus_tag="ACHY09_003295"
     CDS             complement(3980..4195)
                     /gene="bdm"
                     /locus_tag="ACHY09_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFS3886228.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            4540..4971
                     /gene="osmC"
                     /locus_tag="ACHY09_003296"
     CDS             4540..4971
                     /gene="osmC"
                     /locus_tag="ACHY09_003296"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFS3886229.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(5027..5953)
                     /gene="ddpF"
                     /locus_tag="ACHY09_003297"
     CDS             complement(5027..5953)
                     /gene="ddpF"
                     /locus_tag="ACHY09_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS3886230.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLTTHGCEVRQSLTIREDEREIRCWRAL"
     gene            complement(5946..6932)
                     /gene="ddpD"
                     /locus_tag="ACHY09_003298"
     CDS             complement(5946..6932)
                     /gene="ddpD"
                     /locus_tag="ACHY09_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3886231.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLKINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPVLTAYGDNNHRCACWYPQQEVISV"
     gene            complement(6929..7825)
                     /gene="ddpC"
                     /locus_tag="ACHY09_003299"
     CDS             complement(6929..7825)
                     /gene="ddpC"
                     /locus_tag="ACHY09_003299"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFS3886232.1"
                     /translation="MMLSEETSAVRPPKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(7822..8844)
                     /gene="ddpB"
                     /locus_tag="ACHY09_003300"
     CDS             complement(7822..8844)
                     /gene="ddpB"
                     /locus_tag="ACHY09_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3886233.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(8846..10396)
                     /gene="ddpA"
                     /locus_tag="ACHY09_003301"
     CDS             complement(8846..10396)
                     /gene="ddpA"
                     /locus_tag="ACHY09_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS3886234.1"
                     /translation="MKRSILFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSHPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(10410..10991)
                     /gene="ddpX"
                     /locus_tag="ACHY09_003302"
     CDS             complement(10410..10991)
                     /gene="ddpX"
                     /locus_tag="ACHY09_003302"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFS3886235.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPLAASYPLLADQFTCFISPGTQHAS"
     gene            complement(11249..13648)
                     /gene="dosP"
                     /locus_tag="ACHY09_003303"
                     /gene_synonym="pdeO"
     CDS             complement(11249..13648)
                     /gene="dosP"
                     /locus_tag="ACHY09_003303"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFS3886236.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSMLPLK
                     I"
     gene            complement(13673..13837)
                     /locus_tag="ACHY09_003304"
                     /pseudo
     CDS             complement(13673..13837)
                     /locus_tag="ACHY09_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         3406 a         3657 c         3514 g         3477 t
ORIGIN      
        1 cgtaaccaga cggcgcagtc gcgacacatc caccgttttt tcagcctctg ccaggctcca
       61 ggcgttttgc agattcagcc acatttgcgg cgaactgccg atcaccacgg aaagtttaat
      121 cgccatttcc ggagtgagcg ccgcttttcc ggtcagcaat cggcttgccg ttgagggcgc
      181 aatttccatt gctctggcaa actcgcgcag gctgacatta agttcgtcca gtgattcctg
      241 aataatgtcc cccgggcggg gatgattggc cattttcatc agtgatagtc ctcgtaatcc
      301 agtatgtatg catcgccatt gacgaattca aatgtgattc gccagttgcc cgagacagat
      361 atcgcccaaa ttccatcgcg atcgccgctt aacgggtgta gtcggtaacc gggcatatcg
      421 atatcagcaa gacagcttgc tgcatcaatc acagcgagtc ggtggcgtaa tcgtttgact
      481 tgcgtcggta taacgccgga ggttttgccg agaagaaaca agtcacgcaa tcccttgtgt
      541 ctgaaattca ttatcatctc ttcatccttg ttccttcaaa aggaacacta tcactattgt
      601 tccgtgcagc gcaacaaatt tcgctacgtt tttgcgaatt gtgaggggaa atatggggga
      661 ggaaaagaag aagagtgaaa ttgcggaaag cgtctcgcaa aacaggctgc gacccgaaca
      721 tggcagtcgc agcaaaggcc tcttagcggc ggaaatcaat caccatacgg ccacggattt
      781 tgccttcttc catctcggta aagatggtgt tgatgtccgc taacggacgc agggcgactt
      841 tcggcaccac tttgccttcg gcggcaaact ggaaggcttc ggttagatcc tggcgcgtgc
      901 cgaccagcga accgaccact tcaataccat ccagcacaag gcgtgggatg tccaggctca
      961 tagactccgg cggcagaccg acagccacaa cacgaccgcc tgctcgtaca gcatcaaccg
     1021 ccgagttaaa cgcagcttta gctaccgctg tcaccaccgc ggcgtgagcg ccaccggttt
     1081 tttcttgcac aattttggcg gcgtcttcag tgcgtgagtt aatcgctaaa tcagcgccca
     1141 tttcggttgc cagttttaac tgctcatcat tgacatcaat ggcgatcact ttggcgttaa
     1201 agacattctt cgcgtattgc agggcgaggt tacccagacc gccaagaccg tagatagcaa
     1261 tccactgacc gggacgaatt tttgacagtt tcaccgcttt ataggtggtg acccccgcac
     1321 aggtaatgct gctggccgcc gccgagtcca ggccatctgg cacttttacc gcgtaatcgg
     1381 cgaccacgat gcactcttcc gccatcccgc catcaacgct gtatccggca tttttaactg
     1441 aacggcagag cgtttcgtta ccactgttac agtattcgca atgaccgcat ccttcgtaaa
     1501 accacgccac gctggcacga tcgcctggtt ttaatgaggt gacacctggg ccaacttctg
     1561 ccaccacacc gatgccttca tggcccagaa ttacgccggt tttgtcacca aaatcgccat
     1621 tcttaacatg aagatcggta tgacatacac cacaacactc cattttcagc agggcttcgc
     1681 catgtttcag tgagcgcagt gttttatccg taacgtcaac atgatgatcc ttcgtaacaa
     1741 ctgcagcctt catagttcct ccttttcgga tgatgttctg catagcaggt gaggcaaatg
     1801 agatttattc gccactaccc agtatggatg agatctgaaa aagggagagg aaaattgccc
     1861 ggcagctttc accaccgggc gcaggcttag atggaggtac ggcggtagtc gcggtattcg
     1921 gcgtgccaga aattatcatc aatggcctgt tgcagagctt cggcagacgt tttcaccgcc
     1981 acgccttgct gctgcgccat tttgccaacc gcaaaggcaa ttgcgcggga gactttctga
     2041 atatctttca gttccggtag taccagacct tcgccgttca ggaccagcgg cgaatactga
     2101 gcaagcgttt cacttgccga catcagcatc tcatcggtga tacgtgacgc gccggaagca
     2161 ataacgccca gcccgatgcc cgggaaaata aaggcgttgt tacactgggc gatagggtag
     2221 attttatctt tccataccac tgggttaaac ggactgccag tggcgaccag cgcgttacct
     2281 tcagtccagg cgataatgtc ctgcggtgtg gcttccacgc gtgaagtcgg gttagacagc
     2341 ggcatcacga tcggacgcgg acagtgttta tgcatctcac ggataatctc ttccgtaaac
     2401 agcccggtct gtcctgagac gccaatcaga atatctggtt ttacattacg caccacatcc
     2461 agcagtgaca gcacatcgct gtcggtatcc cagtcactga ggttttcgcg cttctgcacc
     2521 agtttggtct ggaaaggcag caggttcggc atcttgtcgg tcagcaggcc aaagcgatca
     2581 accataaaga ctttctgccg cgccgcttcc tcgcttaatc cttcacgctg agtctgggcg
     2641 atgatcattt cggcaatgcc gcaccccgca gagcctgcgc cgaggaagac aatctttttc
     2701 tcgctcagtt gaccacctgc cgcgcggctg gctgcgatca gtgtgccgac cgttaccgcc
     2761 gcggtgccct gaatgtcatc gttaaaagaa caaatttcat tgcgatagcg gttaagtaac
     2821 ggcatcgcat ttttttgtgc gaagtcttca aactgcaaca gtacgtccgg ccagcgttgt
     2881 ttcacagcct ggataaattc atcaacgaat tcatagtatt cgtcgtcagt gatacgcgga
     2941 ttacgccagc ccatatacag cggatcgtta agcagctgtt ggttgttcgt cccgacatcc
     3001 agcaccaccg gaagggtata cgccgggctg atgccgccac aggcggtata gagcgacagt
     3061 ttaccgatcg gaatgcccat cccgccgatg ccctggtcac caagccccag aatacgttca
     3121 ccgtcagtca ccacaatcac tttaatatta tggttcggca cgttttgcag aatatcgtcc
     3181 atattgtggc ggttctggta agagataaac acgccgcgtg aacggcggta gatctcagaa
     3241 aaacgctcac aggctgcgcc gacggttggg gtataaataa caggcatcat ctcatcaaga
     3301 tgattgttta ccagacggta gaagagggtt tcgttagtgt cctggatgtt acgcaggtag
     3361 atgtgtttgt cgatttcggt tttgaatccc tgatactgga tccatgctcg ttccgcttgt
     3421 tcttcgatgg tttcgaccac ttccggcagt aaccccagca ggttgaagtt acggcgttct
     3481 tccatgctga aggcgctgcc tttattcaac aacggaaatt ccagcagtac agggccagcg
     3541 taaggaatat aaagcgaacg ctgtcttttt gtttttggtt ccatgtcact cactcttttt
     3601 tgaatatcca tccctggcac gcttttatcg tctttgcttt accgccaggg cgtcggcccc
     3661 aagtataaag cagataaaaa caaaaacacc attgcgcagg caatggtgtt taatcgtcat
     3721 tgaggactga tggttatgaa ttacttttca gcggggcgtt ttctgcctgt tgggttattt
     3781 accacgctgg atttgtcacc ttcggtaact attttgcgct ggttagaaat tttatggtcc
     3841 agtccaagaa tatgacgtgc ctgacggttc gatttcattt taactcctca atcctgttac
     3901 tagttttaag gacaacatcg ccgtagcgaa gaaacacgtg ctaaacccct aaattaggtt
     3961 gccgatcaag catagcacct taaagcgtag ggtgctgacc actgaccaca taattgatcg
     4021 tttgctggta gatatcactg aggatgtcgt tatcagatgc ttccacccat ttggtcagct
     4081 ccatgagaat gtcatcttca gtgacaacac cgtgctctgc ccgaaggcct tgctcaatgg
     4141 cattaaccag agcgggttct gctgttgaat tttctgcctg ataataagta aacatagtga
     4201 ttctccgtgt ctgtgtattt atggtgtctg ctacggatcg cagatttata aagcacattc
     4261 agcatggcaa atatttgccg cttcgttgtt aagattagtc ctggttgatg atttttatat
     4321 tttaactcca tgatattgat aggtattatt attgtgatga gttgattgat attgatgcaa
     4381 tatctcttga gttatatatt tccggttatc tattgtggaa atttaattta agtgcaggaa
     4441 ttatattttc gccggatttt attcggatta tcctgcttat cctcgtgctg tttctcacgt
     4501 agtctataat ttccttttta agcccacagg agagcaacaa tgacaatcca taagaaaggt
     4561 caggcacact gggaaggcga tatcaaacgc gggaagggaa cagtatctac tgagagcggc
     4621 gtgctgaacc aacagccgta tggatttaac acgcgttttg aaggcgaaaa aggaaccaac
     4681 cctgaagaac tgattggcgc agcgcatgcc gcatgtttct caatggcgct ttcattaatg
     4741 ctgggggaag cgggattcac gccaacatcg attgatacca ccgcagatgt gtcgctggat
     4801 aaagtggatg ccggttttgc gattactaaa atcgcactga aaagcgaagt tgcggtgccg
     4861 ggtattgatg cctccacctt tgacggcatt atccagaaag caaaagcagg atgcccggtt
     4921 tctcaggtac tgaaagcgga aattacgctg gattaccagt tgaaatcgta aagcgttgcc
     4981 ggatgacgcg tcaggcgtgt gattgcctga tgcgttgtta gcgtctctac agcgcccgcc
     5041 agcaccggat ctcacgttcg tcctccctta ttgttaatga ttgccggact tcacatccgt
     5101 gggttgtcag agggcagcgt tcgcggaaaa aacagccttg tggcaacgtg cggtttcctg
     5161 gtagatccgt tttacgtaat gcccattctt cctccagcgg tttatcaatg gcggggaggg
     5221 aatccagcaa taatcgggtg tatggatgtg ccggagctgt cagcacctgt tgcgcgtctc
     5281 ctagttccac aatctgtccg agatacatca ccgccacccg atcgctcata tgacgtatca
     5341 ccgagacatt gtgtgaaatc agcacataag tcagctcgcg attttcctgt agcgttacca
     5401 gtaaattgag gatctgcgcc tgcacggaga tatccagcgc cgaggttggt tcatcaagca
     5461 caatcacgtc aggctgcgaa gagagtgctc tggcaatggc gatacgttgc cgctgcccgc
     5521 cggaaaacgc atgaggcaag cggtcgagat actccggacg aatacccacc tgcacagcca
     5581 gctcctctgc caacgctcgc cgctgttgtt cactactacg cttagcaatc cagagcggtt
     5641 ccgtgatgat gcgccacacc ggtaagcgcg gattaagcga agagagcggg tcctgaaaca
     5701 ccatctgcat aatgcgttgt gatcctgaac ggatgtactg cccgtggctc ggttgcagca
     5761 tacccattaa aagctgtgcg agggtgcttt tgccgcagcc tgactcaccg acgatcccta
     5821 aggtttcacc acgacggatc tgtaaatcaa taccattgat ggcatgaaca tgttccgtag
     5881 ttttaccaag ccagttttta cgggccggga aattgatatg gacgtcgcgt aacgttaata
     5941 atgtatcaga cactaatgac ctcctgctgc ggataccaac aggcgcagcg gtggttgttg
     6001 tcaccatacg ctgttagcac cgggacgttt tcacactgtg cacctgccgc atagcaacga
     6061 tcgcgaaaag cgcagccgtc aggcaaatgg gtgaggtttg gtaccgtccc cggaatagcg
     6121 ggtaatggct ggcgcggtat tccatgttcc ggtgcgcatt gcagcaaacc aatggtatac
     6181 ggatgccggg gatgatggat aacgtctgcc gtcacgccgc tctcaatcac gcttccggca
     6241 tacatcacgt aaacgctatc gcacagttgc gacaccacgg ccatatcatg gctgatgaac
     6301 agtaccgcag ttccactggc gcgggcttta tgtttaagca gacgcagtac ctgcaactgt
     6361 accgtgacgt ccagcgccgt agtcggttcg tcggcaataa tcaattgcgg ctcgcaggag
     6421 aatgccagcg caatcattac ccgctggcgc ataccacctg aaagctcaaa cggatagcgc
     6481 gacataactt ccacggcatc cgggatttgc atctcttcca gcagggcaat cgctttagct
     6541 ctggcttccc gacgacttat tggttgatga tggcggatca cgtccatcat ctgaagacct
     6601 attcgacgtg tcggattaag ggcggtcatc ggttcctgaa agatcatcgc cactcgtgcg
     6661 ccgcgccact gacgaagctg cttttcccga gcgttaagaa catcttctcc cagcagtgaa
     6721 atatgtcccc gatgtacgca ataactgccc gtcggtagca gacgcataat caacattgca
     6781 gtgactgatt tacccgagcc ggattctccc accagaccga caatttcacc gcggttgatt
     6841 ttcaatgaca cattgttgag cgcgtgaacg tcgccgttaa aaccggggaa actcaaatgc
     6901 agttgttgaa tgtccagaac gggttgggtc atgactgctt tcctcctgct ttcgggtcaa
     6961 gcagatcgcg aataccatcg ccaaagagat taaacccgac ggcggtaagc aaaatcgctg
     7021 cccccggaaa tgcgcaatac caccattgat cgagcacata gttgcgacca ttcgccacca
     7081 tcgcccccca ttcagcactc ggttgttgag caccaaggcc aataaatccc aacgtggcgg
     7141 ccattaaaat cgcgctaccg atatccagcg atgcctgcac aatcagcggc ggcagggagt
     7201 tacgtaaaat atgccagttg atcagatgcc agcgtgacgc accaaaggtt ttcgccgcct
     7261 gaacataggt atactggcga acaactaatg tttgcccccg cgccaggcgc acataaaagg
     7321 gaattcgcac aatagcaatc gccagcatgg cgttaaacaa actcggcccg agagcggctg
     7381 ccagtgccat tgtcagcacc agcgaaggaa tcgacagcat aatgtccatg atgcgcatga
     7441 taatcgcgtc tgcgcgtccg ccaagcacac cggatagaca cccgagtagc gaccccatca
     7501 tacctgcaat ggcgaccacc actaatccgg cgagaattga ttgctgactg ccgaccagta
     7561 cgcggctgaa cagatcgcgt cccacttcat cggtgccaaa ccagtgcgct gcagaaggcg
     7621 gcaaaaggcg ggcggttaaa tcaatggcgt tgggatcatg cgtcgccagc cacggtgaaa
     7681 aaatcatcat caatagcatt aatacaatga tgactgcgcc ggtcacggta agcgggctgc
     7741 ctttcagcat ccataccagt tttgcaccgt taaatcgcgt ttgttttggt gggcgcacgg
     7801 ctgacgtttc ctcgcttagc atcattcacc acctccacgt ccgatacgcg gatcaatcca
     7861 caaatagagc aaatccacca ccaggttcac cagcacataa gcaaacgaaa ccacgacggc
     7921 aaagcccatc actgccggga agtcgagcgc ctgtattgat gttactaccc atgcacccat
     7981 tccgggccag gcaaaaacgg tttcggtgag cactgcgcca tacaacaaat cgcccagcgc
     8041 cagtcccaat acggtaatcg aggggatcaa cgcattgggt agcgcataac ataaaacgat
     8101 ataccagccg ggcaagccgc tggcccgggc ggtacgaatg taatcttcac tcaattgttc
     8161 cagcatagct gagcggatct ggcgagcgac aattcccagg tgaacgaacg ccagcgttaa
     8221 tgccggtaag atgagatgtt gcagcgcatt gaagaagact tcaccgttgc cttcaagcag
     8281 cgcatcgagc agataaaagc cggtaacgtg cgttggtgga tccagccagt catcaagcct
     8341 tccgccgccg ggaagaattt gcagatgacc ataaaacagc acaatgacgc ccagcccaag
     8401 ccagaacgca ggtgtggaga ttccggtaat ggccattatt cgcaccagat gatccagcca
     8461 gcgatttcgc cagactgcag agagtatgcc cagcgggatg ccaatcagaa gtgccagcag
     8521 cagggcacaa aaagccagtt ccagcgtcgc cgggaaaaat atgcgcaact cttccagcac
     8581 cggacgcccg gtacgaatgg atgttcccag gtcaccctga aacaggtcgc tgacgtagcg
     8641 gtaaaactgt acgtacagtg gctggtccag tcccagttgc tggcgaatat tatccacgat
     8701 agcatcgctg gcgcggtcac ccgccagtaa ccgcgccgga tcgccgggga tcaggtgcga
     8761 gataataaac gttatcacac agacgcccgc caccacgagc accagccccc agcagcgttg
     8821 gcgtaaaata ctccagaagg tcacgttatt tactcatggt attgatattg aagacctgtt
     8881 ccagcatggg attgaacaca aagcctttca cctctttgtt catcgccagt tggtagtttt
     8941 tctggaacag gtacacataa gcagcgtcat caatgacgat tttctgtgcc tgctggtagt
     9001 cccgggtacg ctgcgtctgg tcggtggtcg ctagcgcatt gcgcagtagc ttatcgacct
     9061 cactgttttc atagaacgag cggttacccg gcagaccttt tttgtctgac tcaaaccagt
     9121 aattcataaa catatacggg tcggcaaaat ccggactcca gttgccaatc gcgatgtcgt
     9181 aatcaccttt acccactctg tcgcgcatgg tggcgttcgc cagcttttcc agcttcacat
     9241 tgatgcccag tttgttgaga ctggattgtg tcgccagagc gatcggttcc cagttcggat
     9301 cattatcaga atagagaaac gtcaggctgg tgggtttgct cgtcacttta tcccattcgg
     9361 ctttggcttt cgtttcgtca tggttgtatt gcattgccgt cgcatcgtat ccccacatgc
     9421 cttccggaat cgggccgcgc atttgttttc cgttaccact cagaatgccg ttaaccatgc
     9481 cctgataatc ggtagaccag gaaatggccc gacgcagatc cgcctgatta agtggcgctt
     9541 tgctgttatt cagatacaga taggtgacgc gcagtgacgg atactctgcc acattgactt
     9601 tattttcctg cttcagggcg ttgagttgat ccaccggcag cgcatcggca atgtcaatgt
     9661 cgccacggga gagctgcagg cgacgggagg cactttcacc gataattttt accgatactc
     9721 gtttaaagtt cggtttattg ccggggtaat gcggatttgg caccagaact aattgctgac
     9781 ctttttgcca gcttttcagc ataaacggtc cggagccagc ggtattttgc gcgaggaaac
     9841 cacgggcatc atccgccgca tgctccttta agaccgccgg attgataatg gatgcaccgt
     9901 cattcgccag cgtgtagagg aacggtgcga atgggtggct aagggtaaac ttcaccgtat
     9961 gttcgtcggg agcatcaatc tttaaatctt tgggaaatgc ttctgctggc ccctggccga
    10021 tttttagtaa ccgctcaaaa gagagtttta cggcttcggc agtgacaggt gtgccatcgg
    10081 caaatttagc attgtccttc agggtgaacg tccactcttt ttgatcgtca gacgctttcc
    10141 agctactagc cagatcgcct tcaacgtcgg ttgagccttt atcaccgtcc gttttgtact
    10201 gaaccagtcg ctgataagac gggtaggtca ctgtccagtc gttattatca attgtcaccg
    10261 ctgggtcgag ggtttgtggg tcggcagctt taccgatcac cagcatatct ttcggtacgg
    10321 cggcgtgcgc aaccggcatt gttgtggcaa ggacgatcgc gagcaatgta ggacgaaaca
    10381 aaatcgatct cttcatgaca gtttcctgat tagctggcgt gctgtgtgcc aggggatata
    10441 aaacaggtga attgatcagc gagtagtggg taactcgctg ccaggggtaa ttcgaagtgc
    10501 caccattcgc tcgggatacc gacaaagcca ccgccagtca taatcgcatt cagcaacagg
    10561 cgattgcgct gagcggcggg cgggacggaa gggtgataag catgggaacg ttcgtgcatt
    10621 tcatcgaacc ctgcacccat atcgaggatg ttcccgtgct catcacgaag cgtcaggtcg
    10681 atcgccgtgc cacggctgtg attagaaccg accgtcacat caacaacata ttgcgggtct
    10741 gggcaggctt gccacaacat cgcctgtgct tgttgtgggc gatacgcatc gtaaatcacc
    10801 agttgtaagc ctgacagctg ggcgatactg atacttttcg ccagcgcagt aatcgcatcc
    10861 ttgtgtaaca gacaacgcgc ttgctgataa atagctttac ctgtgatgtt atcagcgcag
    10921 gcgtatttca attcgatctc cagatcaggg aagatcacgg ctaaatcaac cagttcggtg
    10981 gtatccgaca taaacgattc ctgtataaga caaaaattgc tgcgctttcc gcttatgcag
    11041 atctcatgcc acgccgggat aagcgccaga atgctggctt aaagttatat tttttaactt
    11101 ttgatcaaca tttgtgcagc gtagtgcagt tttggtgcaa gagaggaagt taaggaagga
    11161 atctcccgga atcgtagctg aaatcacagt atttaagtga cagtgtcacg ttaaatgaaa
    11221 acccgcgtgt gcgggcgaga ggaatttgtc agattttcag cggtaacata ctgctcatcc
    11281 agcctggaat ttcttcggcg ggcagggggc gggaaaagaa atatccctga ataacgcgac
    11341 agtggatctt gcgtagcatc tcaaattgct ctttggtttc gacgccttcc gccacgacgg
    11401 ttaaattaag gctttgccca atgctggtaa tggcttcaag taaggcaagg atgcgttttt
    11461 cggtcagaca acgatcgaca aaacttttgt caattttgat ttccgttacc ggaagactga
    11521 ctaagcggga taatccggaa aagcccgtgc caaaatcatc taccgataaa ccgatgccca
    11581 tatcgcgcag gatctgaatg cgtttaaaga tttcggtatc gtgttccatc atcatgcttt
    11641 ccgtgatttc caccgtcagc tggtggccgt cgatacccca ggcttgcatt gcatcagaca
    11701 cctgattagg cagctgatta ctgcgaaagt gcagcgccga caagttaacg gataacgccg
    11761 ggatatgaat attctggcta cgccattctg ctaactgacg gcaagcttcc gcgatgaccc
    11821 agcgcccaat attttcgatt tcaccgatct cttctgcgag aggaataaac cgtgaagggg
    11881 gcacatgacc atgcagggga tcgtgccagc gagcaagggc ttcgatgccg tacagttcac
    11941 ccgtttctgc gaagatttgc ggctggtaaa ccagtttcag ttggttattg ctaattgctt
    12001 ctttcagcgc cgcgcctaaa accaaacgct cttttaccat ttcgttcatc gccgggctga
    12061 agaactgcca gccgttaccg ccatttttgc gaataaaatc cattgcattg tgagcagtgg
    12121 agagcaggta atcgcggttt ttacccacgt cgtagctgat gccaatgctc aaggtgagcg
    12181 ggaatggttt atcgtcaatc attatcggct tgctgaccac attccgtagc tcatcggcga
    12241 tctgcgtaat gttactgacg tcgttttcca ggctcacgag gacaaactgc gtaccttcga
    12301 tacgacagag atattgatcc ggtttgagtt tttcacgaaa gcgattgacc acttccagca
    12361 atgcctgatc ggcccacgca tagccaaggc tatcaatcac atcctgaata tggtcaacac
    12421 cgatgagata caccaccgga gagacggctt tgtcgaccag gtcatcgagg taattgtgca
    12481 ggttattgcg atttggcaga ccggtcatcg gatcaaattg gatgagttgt tcaatatgct
    12541 gacggctttt ttcctgttcc agcgccagcg cggccatatg ctggctgata tctgccacgc
    12601 gttcgataaa ggcgctggtt tctgctcctg acgaggtttt aatttgcagg atccccgcag
    12661 gcgcaccatc acgctgacga atggtcgctg accagctttg cgcattttga acttctgcac
    12721 cgtgcgaaga tgacgcccag tgtatcggca tcccgttgcg cagcgcgaac agcgaaacat
    12781 gcgattcgtt gagtacagat tcgatgttac gacaaatgat ttcccccatt tcatgaaatg
    12841 gcgggctgct gcacatggcg gcgagaatat tgccttcaag ctggcggatt tgcctttctt
    12901 cggtgatatc cgagaatgtc attaccaggt tctgcagatg cgcgagcacg tcataaaccg
    12961 ggctgataga ggctttaatc cagatttttt caccggtgcg cgtcaacagc agaaattcgt
    13021 cctgatcgcg ggcggttttc catagcaact gctgtaaacg aatacggtta tcagcaggga
    13081 attctggaat gttcaggagt gtatcgggct gcataccgct ggcttcgtta atgcagtaac
    13141 caaacatttc ggtaaatgcg cgattgcact gcacaatatg gcgttccgga tcgaggacaa
    13201 tcaccggtcg gtcgagatgg tcaacggcaa taatcaattg tcgggtctgt tctttttgcg
    13261 ccatttctac gctggcatcc cgtaccagcg ccaggtaata aactttcccc tcggcgctca
    13321 ctttcgatag cgcaaaacgg gtccagattt tactgccgtc ttttttctcc agctgcagct
    13381 cccgactcat cccctcgacg cgagctttac caccttcacg gttgtgacga atgtattgag
    13441 gatgcgcagg acgcaaatcc cgcggaatca gcatatcaat gttattgcca atgacttctt
    13501 cacgtttgta cccccagagc ttctctgcgg cggggttgaa aaacatcact tcatcatttt
    13561 cgttaattaa caccgcgccc atcatatttt gctcaagggc ggggaaaaaa atgccatcgg
    13621 cggcattatc cgcatcggtt agcttcatga ttacctctgc atcctggcgc atctaaagac
    13681 tggctttaca gagttcaaca cgggttctac ctcgtctttt ggcgatatac agagcttcat
    13741 cggctatttg aatgaggcgc tcatagtcag gatgaccatt aaacatggcg gcaccgatgg
    13801 aaagtgagag ggcaatatcc tcgccgtttg cggctttaaa ataatgtcga cctttatggt
    13861 taaaccgcag gccaaaatca accaaacaca acgtgcgaga tcgactaact gcaccatatt
    13921 ctcctgaaat atgaagatat actgaaaaga aataagcgac ttaggacagt ttcaatctac
    13981 gctactgttc ttgagaagag tatagcccat cgtaattatt tttcggtgac agcgaatatc
    14041 gtatgttttt tcat
//
LOCUS       DAZUCC010000130        14007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZUCC010000130 DAZUCC010000000
VERSION     DAZUCC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.131"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(66..1424)
                     /gene="plaP"
                     /locus_tag="ACHY09_003305"
     CDS             complement(66..1424)
                     /gene="plaP"
                     /locus_tag="ACHY09_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFS3886237.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1414..1476)
                     /gene="yoeI"
                     /locus_tag="ACHY09_003306"
     CDS             complement(1414..1476)
                     /gene="yoeI"
                     /locus_tag="ACHY09_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFS3886238.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1691..2620)
                     /gene="yeeY"
                     /locus_tag="ACHY09_003307"
     CDS             complement(1691..2620)
                     /gene="yeeY"
                     /locus_tag="ACHY09_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3886239.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2666..3490)
                     /gene="yeeZ"
                     /locus_tag="ACHY09_003308"
     CDS             complement(2666..3490)
                     /gene="yeeZ"
                     /locus_tag="ACHY09_003308"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3886240.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3573..3827)
                     /gene="yoeB"
                     /locus_tag="ACHY09_003309"
     CDS             complement(3573..3827)
                     /gene="yoeB"
                     /locus_tag="ACHY09_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFS3886241.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3824..4075)
                     /gene="yefM"
                     /locus_tag="ACHY09_003310"
     CDS             complement(3824..4075)
                     /gene="yefM"
                     /locus_tag="ACHY09_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFS3886242.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4358..4408
                     /gene="hisL"
                     /locus_tag="ACHY09_003311"
     CDS             4358..4408
                     /gene="hisL"
                     /locus_tag="ACHY09_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFS3886243.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4384..4507
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4554..5453
                     /gene="hisG"
                     /locus_tag="ACHY09_003312"
     CDS             4554..5453
                     /gene="hisG"
                     /locus_tag="ACHY09_003312"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFS3886244.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5459..6763
                     /gene="hisD"
                     /locus_tag="ACHY09_003313"
     CDS             5459..6763
                     /gene="hisD"
                     /locus_tag="ACHY09_003313"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFS3886245.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6760..7830
                     /gene="hisC"
                     /locus_tag="ACHY09_003314"
     CDS             6760..7830
                     /gene="hisC"
                     /locus_tag="ACHY09_003314"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFS3886246.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7830..8897
                     /gene="hisB"
                     /locus_tag="ACHY09_003315"
     CDS             7830..8897
                     /gene="hisB"
                     /locus_tag="ACHY09_003315"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFS3886247.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDLANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8897..9487
                     /gene="hisH"
                     /locus_tag="ACHY09_003316"
     CDS             8897..9487
                     /gene="hisH"
                     /locus_tag="ACHY09_003316"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFS3886248.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLARRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9487..10224
                     /gene="hisA"
                     /locus_tag="ACHY09_003317"
     CDS             9487..10224
                     /gene="hisA"
                     /locus_tag="ACHY09_003317"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFS3886249.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPARRQIPLIKTLVADVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10206..10982
                     /gene="hisF"
                     /locus_tag="ACHY09_003318"
     CDS             10206..10982
                     /gene="hisF"
                     /locus_tag="ACHY09_003318"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFS3886250.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10976..11587
                     /gene="hisIE"
                     /locus_tag="ACHY09_003319"
     CDS             10976..11587
                     /gene="hisIE"
                     /locus_tag="ACHY09_003319"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFS3886251.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11682..12659)
                     /gene="wzzB"
                     /locus_tag="ACHY09_003320"
     CDS             complement(11682..12659)
                     /gene="wzzB"
                     /locus_tag="ACHY09_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFS3886252.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12806..13972)
                     /gene="ugd"
                     /locus_tag="ACHY09_003321"
     CDS             complement(12806..13972)
                     /gene="ugd"
                     /locus_tag="ACHY09_003321"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFS3886253.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         3522 a         3633 c         3562 g         3290 t
ORIGIN      
        1 agccggagcg acaaactccg gctttttgag atttaccgga tgattatgtc atccggtttg
       61 taagattacg ctacgtcttc gtactgcgga accgggttac ggaaactctt ggttacgcaa
      121 gccaggtaaa tcagaccgat agcagcccag atcagaccca gaaccattga gctttcttcc
      181 aggttaaccc acagcgcacc aacggtcagc gcaccacaca tcggcaggaa cagatactgg
      241 aagtgatctt tcagcgtctt gttacgcttc tcacggatcc agaactgcga gatgaccgac
      301 aggttaacga aggtgaacgc caccaacgca ccaaagttaa tcagcgccgt agccattacc
      361 aggtcgaagt tgattgccag cagggcaatc gcgccaacca ggatgatgtt catcgccgga
      421 gtacgccatt tcgggtgtac ataaccgaag aagcttttcg ggaacacgcc gtcacgaccc
      481 attacgtaca tcagacgcgc tacgcctgcg tgcgccgcca taccggacgc cagtacggta
      541 atggtggaga agatcagcgc gccaacctgg aacgctttac ccgcaacgta cagcatgatt
      601 tcaggctgtg acgcatccgg atctttaaag cgagagatat ccgggaagta cagctgcagg
      661 aagtaagttg caaagatgaa gatcatgccg ccgatcagcg cggtcaggaa aatcgcacgc
      721 gggatcacgc gctctgcatc tttggtttct tccgacaggt tgctgatgcc gtcaaaaccg
      781 gtaaaggaga agcacaggat tgtcgccccg gtaatcatcg ggataacatg cgcatcgcca
      841 gaccagaacg gacgagtgct cgccagcgta ccagcgcctt caccttcaaa tacgccataa
      901 acaaccatgc ccagaatcac cgcgatcagc actacctgca acacgacgat tacagtgttg
      961 aagttcgcta cggatttcag actacgcagg ttaaaggcgg tcataaaggc caccagcgcc
     1021 accacaaaca tccacgatgg aatggaaggc accagagctt caaaatagat tttcgccagc
     1081 agaatgttga tcatcggcgc gaacagatag tcgagcagag aagaccaacc caccataaag
     1141 ccgacagtcg ggctaatgga tttctgggcg taagtgtatg cagagccagc agaaggatag
     1201 cggcgaacca gcttcccgta gctcagagcc gtaaacagga tcgcaatcaa tgcgaacgca
     1261 taggctgtcg gcacatgacc atccgtgagg cctgaaacga taccaaaagt atcaaacagc
     1321 gtcatcggct gcatataggc tagacccatc attacaaccg gaactaacgt aagcgtttta
     1381 cgcaattcca cgcgagaggt gtttggagta acgttatgcg acatggtcat tctcctttac
     1441 ggtgataacc gtcgcgtaag caaaaaattg ccccattttt ttggattcct cagcgacaac
     1501 aactgtcgat ttttagtaaa tatctatccg gtacgaagcc cggcctcttg gtatgaatga
     1561 ttggtttgaa gcaaaaaata accgacgcta attaaacgtc ggtttttagt cattttttga
     1621 cagcggcgca ttgtgcctaa atggggggga aatgacaaga gaatgagagg cttgtcataa
     1681 taatttttct ttaaatggct gattttccgt catcagattt tgcataaaca ccggcgatcg
     1741 cactatttgc taaaatttcg tcccgccacc aaccactggc aagccctgcg gtctgttcat
     1801 tccagccaat ccagacgggt tcaaagagag tttgggcgac cactttcttc tcgattaacg
     1861 cgccactatc gagaaaacgt tgcgccagat accgtggtaa atagccgcag ccaaggccgc
     1921 taatttgcag ctccagcttg gttttaaaat cgaaaacggt aatggcttct tgttcgtcga
     1981 gaagttgtga ggccgtagaa gcgcctgcct gcgcagtgtc ccccaccaca atcgcccgat
     2041 atcgtttaat gatacgacgg tttaacggtt cttcttcctg ggctaacgga tgatgaggcg
     2101 cgaccgcaaa gacttgttca agatcgccca accgcgagaa accaaactca ctggaagatg
     2161 gtggctcatg catcgcgcca acaataatat ccgctcgccc ctgagtcagt gcatcccagg
     2221 aaccaccgag tacgccattg ataaacttca ggcgcgtcac gctatgatgt tgataaaaag
     2281 cctcaatgag aggcgcaagc agagaaaaag gaaacgtgtc gtccacgcca atcaccagct
     2341 cattttccca gccttcatgc agtttgatag cctgcttttc cagttctcgc acggtatgca
     2401 gaacttcccg ccctttctct aataacattt ttccggtgcg ggtgaattta gcgcggtgac
     2461 cgctacgatc aagcaattga atattaaggt cgctttccag cttgtgaacg gtataactca
     2521 gagcggacgg cgtcttgtag agtttagccg acgccgccgc aaaactgcct tctttttcta
     2581 aggcatcgag gatcatcaac acatccagca gtggtttcat acacgccccc ttgcagtgta
     2641 tgcggcgtcc tgctggcgtg atcgtttact ccagcggcat taccagcgga tcgggatact
     2701 ggtattcaaa tcccagttca ttacaaatcc gactgccatc aataatctta cctttgccgc
     2761 tgtccagact atttctgaac tgcggaggtt ccagccccag taaacgggcc atctgcggat
     2821 agaaaacatt acgcgcaggg tgagctggcg cacatatatt atagatgtgt ccgcctttag
     2881 gtgcctgtaa caacagagtg atagcaccaa taacatcttc taaatggact aaattaacac
     2941 catgttcacc atcaggggcg gtttttccgg caaagaagcg tccgggatga cgtcccggtc
     3001 cgaccagccc cgcaagacgc agaatatcga ccgaagtacc gggtaaattg tgcagccagt
     3061 cttcgagttc ttctaatact cgtccactgt tggtcaccgg attacgcggg gtggtttctt
     3121 tcaccgtgcc ttgcgcgtcg ccatagacag atgttgagct ggtaaaaata attcggggaa
     3181 tacgatgggc cagcgcgcta tccactaact cttgtaccgc ttgtaaatag aactcatcgc
     3241 cggggccgct acgacgtgcc ggaagcgtaa tgaccagcgc gtcggcatcc atcagggcat
     3301 ccagatcgtc agaatcgcaa actaactcag gctccatgcg aagcagatag ctatcaatgc
     3361 cactcattcg ggccgcttcg acaccatctt gtgtggtttt actcccggtg acttgccagc
     3421 ctcgcgctga aagtgacatc gccagcggca tgcctaacca ccctaacccg acaattgcga
     3481 cctttttcat ccgttatctc ctgatacacc ttagatctat aaggctacgc tagcgtatca
     3541 aaactgacaa ttcattctat gaatgaatct gttcaataat gataacgaca tgctgcaatg
     3601 agtagtgaat cgtcggtaac cgcgtatacc agacggtgct cctctgtaat gcgtcgggac
     3661 cagaaacctg acaaattatg tttcaggggt tctggtttcc ccttaccttc aaatggtgtt
     3721 ctgcgggtat ctttgataag ttcattgatc tttttaacaa ttcgcttatc tgtttcctgc
     3781 cagtacagat aatcatccca tgattcctca gaccagatta gtttcactca atgatgtcct
     3841 tttccgttcc tttgccagat ttcaggctat cgattgagtc catcaatctc cgggcgttag
     3901 cgggggagcg cagtagataa gccgtctcct ccagggagtt gtattcttcg agtgacatca
     3961 gaacacaagc ctctccattc tgacgagtaa taaggatcgg ggcatgatct tcaacggctt
     4021 tcatcattgt tgccgacaaa ttctgacgcg cttcgctgta gctaattgta cgcatgtcaa
     4081 tctcctcttt tgtacagttc attgtacaat gatgggcgtt aattaactat ttattaatta
     4141 gtttgtagat caaggtattg tcagtgagac gaaaatccag gcttcgctat ttttggtgcc
     4201 gtcagcttag aggacagtcc tcttagcccc ctcctttccc cgctcattca ttaaacaaat
     4261 ccattgccat aaaatatata aaaaagccct tgctttctaa cgtgaaagtg gtttaggtta
     4321 aaagacatca gttgaataaa cattcacaga gacttttatg acacgcgttc aatttaaaca
     4381 ccaccatcat caccatcatc ctgactagtc tttcaggcga tgtgtgctgg aagacattca
     4441 gatcttccag tggtgcatga acgcatgaga aagcccccgg aagatcacct tccgggggct
     4501 tttttattgc gcggttgata acggttcaga caggtttaaa gaggaataag aaaatgacag
     4561 acaacactcg tttacgcata gctatgcaga aatccggccg tttaagtgat gactcacgcg
     4621 aattgctggc gcgctgtggc attaaaatca atcttcacac ccagcgcctg atcgcgatgg
     4681 cagaaaacat gccgattgat attctgcgcg tgcgtgacga cgacattccc ggtctggtaa
     4741 tggacggcgt ggtagacctt gggattatcg gcgaaaacgt gctggaagaa gagctgctta
     4801 accgccgagc ccagggtgaa gatccgcgct actttaccct gcgtcgtctg gatttcggcg
     4861 gctgccgcct ttcgctggca acgccggttg atgaagcctg ggatggcccg ctctccttaa
     4921 acggtaaacg tatcgccacc tcttatcctc acctgctcaa gcgttatctc gaccagaaag
     4981 gcatctcttt taaatcctgc ttactgaacg gttctgttga agtcgccccg cgtgccggac
     5041 tggcggatgc gatttgcgat ctggtttcca ccggtgccac gctggaagca aacggcctgc
     5101 gcgaagtcga agttatctat cgctcgaaag cttgcctgat acaacgcgat ggcgaaatgg
     5161 aagaatccaa acagcagctg atcgacaaac tgctgacccg tattcagggc gtgatccagg
     5221 cgcgcgaatc aaaatacatc atgatgcacg caccgaccga acgcctggat gaagtcatcg
     5281 ccctgctgcc aggtgccgaa cgcccaacta ttctgccgct ggcaggtgat caacagcgcg
     5341 tagcgatgca catggtgagt agcgaaaccc tgttctggga aacgatggaa aaactgaaag
     5401 cgctgggtgc cagttcaatc ctggtcctgc cgattgaaaa aatgatggag tgatcgccat
     5461 gagctttaac acaatcattg actggaatag ctgtactgcg gagcaacaac gccagctgtt
     5521 aatgcgcccg gcgatctccg cttctgaaag cattacccgc accgttaacg atatcctcga
     5581 taacgtgaaa gcacgcggcg atgaggccct gcgtgaatac agcgcgaaat ttgataaaac
     5641 cacggttacc gcgctgaagg tgtctgctga ggagatcgcc gccgccagcg aacgcctgag
     5701 cgacgagcta aaacaggcga tggcggtggc agtaaagaat attgaaacct tccacactgc
     5761 gcaaaaactg ccgccggtag atgtcgaaac gcagccaggc gtgcgttgcc agcaagtcac
     5821 gcgcccggtc gcttcagttg ggttgtatat tcctggcggc tccgccccgc tcttctcaac
     5881 ggtattaatg ctggcaactc cggcgcgtat tgcgggctgt aaaaaagtgg tgctgtgctc
     5941 accgccgccg attgccgacg aaattcttta cgcggcgcag ctgtgcggtg tgcaggatgt
     6001 gtttaacgtc ggcggcgcac aggccattgc cgcactggcg tttggtacgg aatctgtgcc
     6061 gaaagttgac aaaatcttcg ggccaggtaa cgcctttgtc accgaggcaa aacgtcaggt
     6121 gagccagcgt ctggacggtg cggcgatcga tatgcccgca ggcccgtcgg aagtgctggt
     6181 gattgctgac agcggcgcta cgccggattt cgtggcttct gatttgcttt ctcaggctga
     6241 acacggcccg gactcgcagg tgattttact gacgcccgat gctgatatgg cgcgtcgcgt
     6301 tgccgaggct gtcgaacgcc aactggcaga actgccgcgt gccgaaaccg cccgccaggc
     6361 actgaacgcc agccgcctga tcgtgactaa agatttagcg cagtgcgtag agatctccaa
     6421 ccagtacggc ccggagcacc tgatcatcca gacccgcaac gcccgcgatc tggtcgatgg
     6481 catcaccagc gccggttcgg tatttcttgg tgactggtca cccgaatcgg cgggtgatta
     6541 cgcctccggc accaaccacg tactgccgac ttacggttac accgccacct gttccagcct
     6601 cgggctggca gatttccaga agcgtatgac cgtgcaggaa ctgtcgaaag aaggtttctc
     6661 cgcgctggct tcgaccatag aaacactggc cgccgccgag cgcctgaccg cccataaaaa
     6721 tgccgttact ttgcgtgtta acgcccttaa ggagcaagca tgagcaccgt gactattacc
     6781 gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc agtcggcgcg tcgtctgggc
     6841 ggtaacggcg acgtctggct gaacgccaac gaatacccca cagccgtgga gtttcagctc
     6901 actcaacaaa cgctcaaccg ctatccggaa tgtcagccaa aagcggtgat tgaaaattac
     6961 gcgcaatatg caggcgtaaa accggaacag gtgctggtca gccgtggcgc ggacgaaggt
     7021 attgaactgc tgattcgcgc tttttgcgaa ccgggtaaag acgccatcct ctactgcccg
     7081 ccaacgtacg gcatgtacag cgtcagcgct gaaaccattg gcgtcgagtg ccgcacagtg
     7141 ccgacgctgg acaactggca actggactta cagggcattt ccgacaagct ggacggcgta
     7201 aaagtggtct atgtttgcag ccccaacaac ccgaccgggc aactgatcaa cccgcaggat
     7261 tttcgcactc tgctggagtt aacccgcggt aaagcgattg tggttgccga tgaagcctat
     7321 atcgagtttt gcccgcaggc atcgctggct ggctggctgg cggaatatcc gcacctggct
     7381 attttgcgca cactgtcgaa agcttttgct ctggcgggcc ttcgttgcgg atttacgctg
     7441 gcaaacgaag aagtcatcaa ccttctgatg aaagtgatcg ccccctaccc gctctcgacg
     7501 ccggtcgccg acattgcggc ccaggcgtta agcccgcagg ggatcgtcgc catgcgcgaa
     7561 cgggtggcgc aaattattgc agaacgcgaa tatctgattg ccgcattgaa agaaatctcc
     7621 tgcgtggagc aggtttttga ctctgaaacc aactacattc tggcgcgctt taaagcctcc
     7681 agcgcagtgt ttaaatcttt gtgggatcag ggcattatct tacgtgatca gaataaacaa
     7741 ccctctttaa gcggctgcct gcgaattacc gtcggaaccc gtgaagaaag ccagcgcgtc
     7801 attgacgcct tacgtgcgga gcaagtttga tgagtcagaa gtatcttttt atcgatcgcg
     7861 atggaaccct gattagcgaa ccgccgagtg attttcaggt ggaccgtttt gacaaactcg
     7921 cctttgaacc gggcgtgatc ccggagctgc tgaagctgca aaaagcggga tataagctgg
     7981 tgatgatcac caatcaggat ggtctgggaa cacaaagttt cccgcaggcg gattttgatg
     8041 gcccgcacaa cctgatgatg cagatcttca cctcgcaagg cgtgcagttt gatgaagtgc
     8101 tgatttgccc gcacctgccc gccgatgagt gcgactgccg taaaccgaaa gtaaaactgg
     8161 tggagcgtta tctcgctgag caagcgatgg atctcgccaa cagttatgtg attggcgatc
     8221 gcgcgaccga tattcaactg gcggaaaaca tgggtattaa tggtttacgc tacgaccgcg
     8281 aaaccctgaa ctggccgatg attggcgagc aactcactaa acgagaccgt tacgcccatg
     8341 tagtgcgcaa caccaaagag acgcagattg acgttcaggt atggctggat cgcgaaggtg
     8401 gcagcaagat taacaccggc gttggcttct ttgatcatat gctggatcag atcgccactc
     8461 acggcggttt ccgcatggaa atcaacgtca aaggcgacct ctatatcgac gatcaccaca
     8521 ccgtcgaaga taccggcctg gcgctgggtg aagcgttaaa aattgccctc ggcgataaac
     8581 gcggtatttg ccgctttggt tttgtgctgc cgatggacga atgccttgcc cgctgtgcac
     8641 tggatatctc tggtcgccca cacctggaat ataaagctga atttacctac cagcgcgtcg
     8701 gcgatctcag caccgagatg atcgagcact tcttccgttc gctctcttac accatgggcg
     8761 tgacgttgca cctgaaaacc aaaggtaaaa acgatcatca ccgtgtagag agtctcttta
     8821 aagcctttgg tcgcaccctg cgccaggcca tccgcgtgga aggcgacacc ctgccctcgt
     8881 cgaaaggagt gctgtaatga acgtggtgat ccttgatacc ggctgcgcca atttgaactc
     8941 ggtgaagtca gccattgcgc gtcacggtta tgaacccaaa gtcagccgtg acccggacgt
     9001 cgtgttgctg gccgataaac tatttttacc cggcgtaggc accgcacaag cagcgatgga
     9061 tcaggtgcgt gagcgcgagc tgtttgatct catcaaagcc tgtacccaac cggtgctggg
     9121 catctgctta gggatgcaac tgctggcgcg gcgcagcgaa gagagcaacg gcgtcgattt
     9181 gctgggcatt atcgacgaag acgtgccgaa aatgaccgac tttggtctgc ccctgccgca
     9241 tatgggctgg aaccgcgttt acccgcaggc aggcaaccgc ctgtttcagg ggattgaaga
     9301 cggcgcgtac ttttactttg ttcacagcta cgcaatgcca gtcaatccgt ggaccatcgc
     9361 ccagtgtaat tacggcgaac cgttcaccgc ggcggtacaa aaagataact tctacggcgt
     9421 gcagttccac ccggagcgtt ctggtgccgc tggcgctaag ctgctgaaaa acttcctgga
     9481 gatgtgatga ttattccggc attagattta atcgacggca ccgtggtgcg tctccatcag
     9541 ggcgattatg gcaaacaacg cgattacggt aacgacccgc tgccacgctt acaggattac
     9601 gccgcgcagg gtgccgaagt gttgcatctg gtggatctga ccggggcaaa agatccggct
     9661 agacgtcaaa tcccgctgat taaaaccctg gtggcggacg ttaacgttcc ggtgcaggtt
     9721 ggcggcggcg tgcgtagcga agaagacgtg gcggcgttac tggaagcggg cgttgcgcgc
     9781 gtggtggttg gctccaccgc ggtgaaatca ccagaaatgg tgaaaggctg gtttgaacgt
     9841 ttcggtgccg atgccttagt gctggcgctg gatgtccgta ttgacgagca aggcaacaag
     9901 caggtggcgg tgagcggctg gcaagagaac tcgggcgttt cactggaaca actggtggaa
     9961 acctatcttc ccgtcggcct gaaacatgtg ctatgtaccg atatctcgcg cgacggcacg
    10021 ctggcaggtt ctaacgtctc tttatatgaa gaagtgtgcg ccagataccc gcaggtggca
    10081 tttcagtcct ccggcggcat tggcgacatt aacgatgttg ctgccctgcg aggtactggc
    10141 gtgcgcggcg taatagttgg tcgggcatta ctggaaggta aattcaccgt gaaggaggct
    10201 atcgcatgct ggcaaaacgc ataattccat gtctcgacgt tcgtgatggt caggtggtga
    10261 aaggcgtaca gtttcgcaac catgaaatca ttggcgatat cgtgccgctg gcaaaacgtt
    10321 acgccgaaga aggtgcagac gaactggtgt tctacgatat caccgcttcc agcgatggcc
    10381 gtgtggtaga taaaagctgg gtatctcgcg tggcggaagt aatcgacatt ccgttttgtg
    10441 tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat tctttccttt ggcgcggata
    10501 aaatctccat taactcccct gcactggcag acccgacgtt aattactcgc ctggcggatc
    10561 gctttggcgt gcagtgtatt gtggtcggca ttgatacctg gtacgacgcc gaaaccggta
    10621 aatatcatgt gaatcaatat accggcgatg aaagccgcac ccgcgtcact cagtgggaaa
    10681 cactcgactg ggtgcaggaa gtgcaaaaac gcggtgccgg agaaatcgtc ctcaacatga
    10741 tgaatcagga cggcgtgcgt aacggttacg acctcgaaca gctgaaaaaa gtgcgtgaag
    10801 tttgccacgt cccgctgatt gcctccggtg gcgcgggcac catggaacac ttcctggaag
    10861 ccttccgcga tgccgacgtt gacggtgcgc tggctgcttc cgtattccac aaacaaataa
    10921 tcaatattgg tgaattaaaa gcgtacctgg caacacaggg cgtggagatc aggatatgtt
    10981 aacagaacaa caacgtcgcg aactggactg ggaaaaaacc gacggactga tgccggtgat
    11041 tgtgcaacat gcggtatccg gcgaagtgct aatgctgggc tatatgaacc cggaagctct
    11101 ggacaaaacc ctcgaaagcg gcaaagtcac cttcttctcg cgcactaaac agcgactgtg
    11161 gaccaaaggc gaaacgtcgg gcaattttct caatgtggtg agtattgccc cggactgcga
    11221 caacgacacg ttactggtgc tggcgaatcc catcgggcca acctgccaca aaggcaccag
    11281 cagctgcttc ggcgacaccg ctcaccagtg gctgttcctg tatcaactgg aacaactgct
    11341 cgccgagcgc aaatctgccg atccggaaac ctcctacacc gccaaactgt atgccagcgg
    11401 caccaaacgc attgcgcaga aagtgggcga agaaggcgtg gaaaccgcac tggcagcaac
    11461 ggtacatgac cgctttgaac tgaccaacga ggcatctgat ttgatgtatc acctgctggt
    11521 gctgttgcag gatcaggatc tggatttaac gacggtaatt gagaacctgc gtaaacggca
    11581 tcagtgagtt gcgggtgatc agatgcgatg ttgttgtcgc atccggcaaa aaaaacgggc
    11641 aaggtgtcac caccctgccc gttttcttta aaaccaaaag attacttcgc gttgtaatta
    11701 cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag cagcactgcc
    11761 agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt cggtttcatc
    11821 acatagcggt aagcatgaat atcaagatca tcaaccttta agttttcaat atccaacagg
    11881 ttttgacgag tctgatagta gtttggcgag aacaccaacg gacgagtcgc ctcatgctta
    11941 atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt cacatcttca
    12001 gtctgctgaa cctgtggctt tgtcacctgc gcctgattcg catactgcaa cgcttcctga
    12061 atctgacgga tacgcagatc tttctgctct tgcgcgacta cttcctgagt tcttaaagag
    12121 tcctgcaagt ttttcatacg cagcgcgatg ttgtctttca ggtctttttc cagttcctca
    12181 ttaacctggt cgtcaacttg ctggatatac ttcgccagct gcttctgagc accttcaggt
    12241 gtttcaccaa cataagacac cgctaaaggt aatgactgat ttttaacggt aggctcaatg
    12301 gtcagctttt ctgcttcttc ctgattatcc agcgtttccg ctaatgctga aaatgcagtg
    12361 ctgtaacgtc cgataagtga agcctgaatg tcggagactt tcggtacagc cgatccgtag
    12421 atcacattca tcgcgttggt ataaccagcg atttgcccca catcaggttg agtaacgata
    12481 gctgtggaag tccacttttc tttggcaacg gccagatacc ctatagccag ggcaatagca
    12541 acaatgacag aaatgatgat agtcatcttg ccgcgccaca actgcaccag taaatcaatc
    12601 aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc tactctcatc
    12661 attaccctaa cggacgaaat aattttaagt cgccatagtt tagcgataat cgccatgaat
    12721 gctataggat aaatgataaa aagaatgaat tattacaaag ggaacatgag agaatcaggg
    12781 cgaaaatcgc cctgataaca ggatgttagt cactgccaaa gagatcgcgg gtgtagactt
    12841 tgtctgccac atccttaagc tcttctgcca tacggttgga aataataacg tcggcttgtt
    12901 gtttgaaggt ggcgagatca cgttccagtc gagagttgaa gaatgaatct tctttcatca
    12961 ccggctcgta gatgatcact tcaacacctt ttgccttgat acgtttcata atcccctgaa
    13021 tggaagacgc gcggaagtta tctgacccgc tcttcataat cagacgataa atacccacca
    13081 cttgcggttt gcgtgacaaa atggcatcgg caataaaatc tttacgcgtg cggtttgcat
    13141 cgacaattgc cgagatcagg ttattcggca cagactggta gttcgccagt aactgcttgg
    13201 tatctttcgg tagacaataa ccaccatagc caaacgacgg attgttgtaa tggttgccaa
    13261 tacgcggatc gagacaaaca ccttcgataa tctgacgagt attcagacct aaactttctg
    13321 catagctatc cagctcgtta aagtatgcta cgcgcatcgc cagataggta ttagcaaaca
    13381 gtttaatcgc ttctgcttca gtggagtcgg taaacagggt cgggatattt tgcttaatcg
    13441 cgccttcctg taataacgcc gcaaaacgtt ctgcgcgttc tgaacgctca ccgatgacaa
    13501 tacgtgacgg atgaaggtta tcgtaaagag ctttaccttc acggagaaat tccggagaga
    13561 aaataatatt ttcagtacga tatttcttat gcatcgcttc ggtaaaacca acgggaaccg
    13621 ttgatttgat gaccataacc gcataaggat ttatctcaac tacgtcttta attactgatt
    13681 ctacactcga tgtattgaaa taattagttt taggatcata gtcggttgga gtggcgatga
    13741 tgacgtaatc agcatcccga taggcttcat ttttatctaa tgtggcatta aagtgtattt
    13801 tatctgattg caaaaactgc tgaatttcct tatcaacaat aggagatatc cgatcattca
    13861 gcatagcaac gcgtgacggt aaaatatcta atgccacaac ctcatgattt tgtgcgatta
    13921 gaagcccgtt tgacaagccg acatagccag taccggaaat ggtgattttc atttcatgct
    13981 ctcagaatta acttaactgt gaatcat
//
LOCUS       DAZUCC010000131        13860 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZUCC010000131 DAZUCC010000000
VERSION     DAZUCC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.132"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            70..960
                     /locus_tag="ACHY09_003322"
     CDS             70..960
                     /locus_tag="ACHY09_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886254.1"
                     /translation="MPPHKIRQEWLTLDEAAEVIRQHNITLLPADLIRHALHGDLSLS
                     VYFPSPVLLRPLNPPCRFACRFSKPPFPPSQCNKPGNRKVMANSPPCRRDPTLQLTTS
                     HLWNLPLIGIERLLLHQQLAKTLNRPCPNDTHCCNQIGIVVCDENDSYYQLCCRKSLR
                     ELLLELLATQNNLQEELHLLIGQLYGNGHNDLKSRLSLSVCYLQDTLPPDALFIIRRT
                     HLEAFLTRHQPESKERITSALSRLLWLACKHNPQLDDEMLEHPYKLLNIFECWAREEG
                     MHLPLSPETLKTALQRGAPP"
     gene            1099..1293
                     /locus_tag="ACHY09_003323"
     CDS             1099..1293
                     /locus_tag="ACHY09_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3886255.1"
                     /translation="MNSDRFLRLRQVEDKIGFGKSWIYRQIQLQQFPPSIRLNSRHVA
                     WLESEVDAWIHQRIRLTRDV"
     gene            1310..2593
                     /locus_tag="ACHY09_003324"
     CDS             1310..2593
                     /locus_tag="ACHY09_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFS3886256.1"
                     /translation="MCASHLLNRWLHGASNTGEQEMVQVSLEQIQPFSLNPRVTRNPG
                     YDVLKASILARGLDNPPVLTRRPGDEKYMLASGGNTRLAILNELWQETQDERYHRVCW
                     PFRPWPETLSPQQGEVQCLIGHLAESDLHNGLMFIERAEGVLHLRDLYLEAGIECPTQ
                     LALAEQLTRDGYPVSQSQISRMLQTVDWLLPCIPNALYGGLTRKVIDRLLSLRSAAEQ
                     VWVKLMPLERQPEFADLFSTALAIFNGEPEAVVPQLVQDELLGEMSCRSGMSWNTLLA
                     ELTDGQSKRQVLLGPPAEKVLWPPPPEVEKAPLPEPEPPTEPAAPDEPKPSPLVHTAQ
                     SLCACWGLDACIQPDDSAPAGFTLAPCHDFPHPAAQRCYQVLAALNGDTSTPMPALEL
                     LLSPDYNNAQIQQLMQLISLCRQRRLREVRHDTLS"
     gene            2577..3134
                     /locus_tag="ACHY09_003325"
     CDS             2577..3134
                     /locus_tag="ACHY09_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFS3886257.1"
                     /translation="MTPSVNQAVLTHIVQALKEGQIRYCESLGFSPQELCELTRLTAD
                     DILFLSNSAVQFITTHIDHEMLGRMLARMEQERLFQQRLEEALSLGASIEFINHYFGL
                     STPEVCARRRLIGLFVRQGRNNAPDEQVETLVWNEWQKTGVKKLDSPEALTAMMAMAR
                     EHDLSLTVIWNLLRQWTQEKLLHEE"
     gene            3124..3996
                     /locus_tag="ACHY09_003326"
     CDS             3124..3996
                     /locus_tag="ACHY09_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFS3886258.1"
                     /translation="MKSDDRLLFLGNPQQSVPKRLLLARNLSPRDKFAWQLLRLHARD
                     DGSGVFPSYNELQGWLSDQPEGEKASRSTVSNALMMLRITRWLSLCQRLRDEQSGRIT
                     GNLYALHDAPLTHADACRMDSEYLSLLTACSRHRNRRIRNIAIGLLLTLQQPVDGGRT
                     CTESPLKTAPGLNRGLSKKCPSLQLRLSNVAGAGGQPEPHCVHTGTDLNNKHQKRTPD
                     LHWPVDNPFSASERRAAERAMRNLDPALCQQVLDDCLWRIAAHDIHRPLAYLLATLEK
                     ARRGEFNQLRRRRR"
     gene            4156..4767
                     /locus_tag="ACHY09_003327"
     CDS             4156..4767
                     /locus_tag="ACHY09_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFS3886259.1"
                     /translation="MPGTTLFSDIRITLHTRVAARLWQAHPTGMLLCLALLRRLLRAE
                     EADDPWAAHWLKQLRIRLNLIAHLLKQKNRRLDHAFASLPGAIHTTQVSNPSPTEFIL
                     PLALFSPPGSRLLQQLIDYDLLVRRTLLAWHLGLITQAEKRDFIATIPRLMLQVFSFV
                     NRFRTTGVTRADVRANSPLAQSMAHKLGNLPKKMLTEIQRDVQ"
     gene            4757..5182
                     /locus_tag="ACHY09_003328"
     CDS             4757..5182
                     /locus_tag="ACHY09_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HFS3886260.1"
                     /translation="MFNDDDDERSARKSSLKGLLKAFKGKGELRALVQELQALRLQLQ
                     QNQPVLKQAVYELPGRNVPLILCSNPARSGVSYLRWRNLDNNRSGSVALQEAIQQPDA
                     SDELRDALMALEWRRHVLNTQLSVVMFMLRRIQPLTTLV"
     gene            5374..7179
                     /locus_tag="ACHY09_003329"
                     /pseudo
     CDS             5374..7179
                     /locus_tag="ACHY09_003329"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            7287..7469
                     /locus_tag="ACHY09_003330"
     CDS             7287..7469
                     /locus_tag="ACHY09_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886261.1"
                     /translation="MAKNTGDGYRKGSVTDRTQVQNPQNGNYTKRDTTTGRFIDQKQD
                     GTPFKGVAKEVDDRRK"
     gene            7753..8196
                     /locus_tag="ACHY09_003331"
     CDS             7753..8196
                     /locus_tag="ACHY09_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFS3886262.1"
                     /translation="MNDTHYFNLYTSGLGYLNRIREVTPKKGEPFLCCDIAALRGEAS
                     NAEHTWFDCRVSGKEAQKLVRRCIDACEQKRKVLIQFRLGDLYADTFVYSKGDRKGET
                     GVSLKAHLLHIGLIKIDGEEVYRAEREAEPQPAPAALPAEPVAQF"
     gene            8270..8650
                     /locus_tag="ACHY09_003332"
     CDS             8270..8650
                     /locus_tag="ACHY09_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886263.1"
                     /translation="MNTVLNSREEALDRGKLINLDDEALDAGLPLDLAITLPAHKHCL
                     EDRNDNAIRDLLKGVEMAAVVAFISHQPPQRTIRIDYRSVLTQKTNGSTQQQTVRLTL
                     RVHFSEQGIPIAATLGLMGERFPY"
     gene            8764..9420
                     /locus_tag="ACHY09_003333"
     CDS             8764..9420
                     /locus_tag="ACHY09_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilL"
                     /protein_id="HFS3886264.1"
                     /translation="MKRLLTALLLFIFNGVAASPATPEVVRYDRYLLVSTSPQQPPLE
                     QLTTLSVPPGFHPTVGEALQYLLRDSGWSLCLSDARRAQLYGLPLPLNQYHTGPLRLK
                     AALQLLAGPAWRMTADENRREVCFTSDHSLPQTVAYRHPVSLHPLGPLTAPFFLAGTV
                     QRGDIRLAVIVPPEALYLHQVVWLRIGEQWHGWRLARFDERRAVFRHGKRQLTLKVPE
                     "
     gene            9424..10098
                     /locus_tag="ACHY09_003334"
     CDS             9424..10098
                     /locus_tag="ACHY09_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFS3886265.1"
                     /translation="MKRLILLLLFTASTWAQPLQVQTSQNVGSALQPLTTQQQATVWG
                     ITPEEWQRYLTLKQQARGVWSPGLDPLTTLGVEADTDHERQRFAELLVRQEYQRLEKE
                     LAFQRAYDAAWQRLYPGLTPLKTTSASTSRVSLFVKDDCPPCDSLLRTLLAQQRPLDI
                     WLVGSEGDDNRVRRWAMAHGIDAKLVGEHSITLNHDAGRWLLLGQSKIPVALERKGEQ
                     WREVLP"
     gene            10077..10595
                     /locus_tag="ACHY09_003335"
     CDS             10077..10595
                     /locus_tag="ACHY09_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFS3886266.1"
                     /translation="MARGPALILLLPCLALAQPLPEAYLRTAEHFRVPPELLWAVTQT
                     ESGTNLSGRHLPWPWTLNISGTGYRYASREQACQALLVAIRRTSPKRIDVGLGQINIG
                     WHQAYFDSPCDALYPLQNLSLTAQLLRQRYDERPGSWLNAAARYHRPAGGAPAARYRK
                     LVSRHLQQASPP"
     gene            10592..12610
                     /gene="traD"
                     /locus_tag="ACHY09_003336"
     CDS             10592..12610
                     /gene="traD"
                     /locus_tag="ACHY09_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFS3886267.1"
                     /translation="MSRYALEALLRPPVEFYSTVIAGCGALICLTQAPLLMLTPWQAR
                     MMASALMLLALRRGWQGWQIVRYQRNLLRLPRYALTSRKIPVSRQRLFLGKGFRWQPI
                     HTQRLYDCLQPEAARWLRPGWCYRLARHIEKRSEHTLPGLARLLSADRWLNPVRPLPP
                     VGGNPWLHGVELKECDVTLPLSERPGHTLVLGTTRVGKTRLAELLATQDIRRGEVVIV
                     FDPKGDADLLKRMWTEAQRAGRGDRFILFHLAYPEFSARYNAIGRFGRISEVASRLTG
                     QLSGEGNSAAFREFAWRFVNIIARALVALGQRPDYQLISRHVVNIDALFIDYARIVLP
                     RHNTRSWEVVVQLAERVNDKNTPRHMLGRDPHVVALEQYLNAEKLYDPILDGLLSAVR
                     YDKTYFDKIVASLLPLLEKLTTGTLAALLAPDYHDLDDARPILDWQQAIRQKAVVYVG
                     LDALSDSVVAAAVGNSMFADLVSVAGHLYKFGLNDGLPGNSEKPPPINLHCDEFNELM
                     GEEFIPLVNKGGGAGIQVTAYTQTLSDIQARIGSAPKTGQVVGNFNNLIMLRVRETAT
                     AELLTKQLPQVEVRSRQVSSGVTDTPGGINGFTSNTCEQVTTRNVPLLDTAALIQLPK
                     GQAFALLEGGQLWKIRLPLPVADKQCPLPDSVAQLAAWMQARQQGLTE"
     gene            12607..13254
                     /locus_tag="ACHY09_003337"
     CDS             12607..13254
                     /locus_tag="ACHY09_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFS3886268.1"
                     /translation="MSELRPPPSRPPQGPIGWLLSLAGRIIGLVIGTLVLRVVLELVG
                     LYFWWPQEGSRHVFQVMRQEKVELIIAVQPHPLGKEIRVLLEHGITRVNDVRHKAVSR
                     LFHEPVTVLAYTMISFALRLTWLVIMLPLLCLSAVTGLTEGLVHRDLRRFGSGLESIF
                     LHRYVIRCGSSVTTTLWVVYLAQPFSLPAMLVLLPAALWSGIAVWMAAGSFKRWL"
     gene            13320..13565
                     /locus_tag="ACHY09_003338"
     CDS             13320..13565
                     /locus_tag="ACHY09_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFS3886269.1"
                     /translation="MGVMSVRLNNNLSTQLEALSKATGRSKSWLANQAIEDYVAREAW
                     QVAEIEQAIQEADAGDFATSDEVDKLFQRLGVKPDGN"
     gene            13555..13839
                     /locus_tag="ACHY09_003339"
     CDS             13555..13839
                     /locus_tag="ACHY09_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3886270.1"
                     /translation="MEIKWLRKAIANLEAEFRYIAEDDPQAAERFVNEVWRLTQLLKE
                     QPAMGREGRVPETRELILHNYPYIIPYRVRNNIIQVLRVFHTHRRLPVNW"
BASE COUNT         2962 a         4310 c         3883 g         2705 t
ORIGIN      
        1 ctcaccttac ctcgctttag ttggcttttt tagatagtac tcatcatatg taactaatag
       61 gagttaccca tgcccccaca caaaattcgc caagagtggc tgacgctgga tgaggctgca
      121 gaagtcatcc ggcagcacaa catcacgttg ctgcccgccg acctgatccg ccatgccctg
      181 cacggcgatc tgtcgctctc ggtctacttc ccttcgcccg ttctgctgag accactcaat
      241 ccgccttgcc gctttgcctg ccgattcagt aagcctcctt ttccaccttc gcagtgcaac
      301 aaacccggca accgcaaagt gatggcaaac tcgccgccgt gcagacgcga cccaactctg
      361 caactgacca ccagccatct gtggaatttg ccgttaatcg gcattgagcg cctgctgctc
      421 caccagcaac tggcgaaaac gttgaatcgc ccttgcccca atgataccca ttgctgcaac
      481 cagattggca tcgtggtctg cgatgaaaac gacagctact accaactctg ttgccgcaaa
      541 tcgttgcggg agcttttact ggaactgctc gccacgcaaa acaacctcca ggaagagctt
      601 catctgctga ttggccagct atatggtaat ggccacaatg acctgaaatc ccggctcagt
      661 ctgtccgtct gctacctgca ggacacgctg cctcctgacg cactgttcat catccgccgc
      721 actcatctcg aagccttcct gacccgtcat cagccagaat caaaggagcg gatcacctcc
      781 gcgctttcgc gtttgctgtg gctggcctgc aagcacaacc cgcaactgga cgatgaaatg
      841 ctggagcatc cctacaaact gctgaatatt ttcgaatgct gggcgcgtga ggaaggtatg
      901 cacctgccgc tgagccccga gacgttgaaa accgcactcc agcgcggcgc accgccgtaa
      961 cgcgatcaac taaccctttc aactaacaac tctcactaac ccagataagg atttttactg
     1021 cctggcaggg acgctgctgc tttgataacc acgcctatcg tgctgatgtg aacgaatctg
     1081 gacacaggag gaatggttat gaatagcgac agatttttac gcttacgcca ggtggaagat
     1141 aaaatcggct tcggtaaatc gtggatttat cgacaaatcc agctgcaaca gtttccgccg
     1201 tccattcgac tcaatagccg ccacgtcgcc tggctggaaa gcgaggtgga cgcgtggatc
     1261 caccagcgca tccggcttac ccgcgatgtc tgaatgccgg aggaagatta tgtgcgcttc
     1321 acacctgctc aaccgctggc ttcacggcgc cagcaatacc ggagaacagg aaatggtcca
     1381 ggtcagcctg gagcaaatcc agccgttctc cctcaatccc cgcgtcaccc gcaaccccgg
     1441 ctatgacgtg ctgaaggcgt caattcttgc ccgtgggctg gataacccgc cggtactgac
     1501 ccgccgcccc ggtgatgaaa aatatatgct cgccagcggc ggaaatacgc gcctcgccat
     1561 cctcaatgaa ctgtggcagg agacgcagga tgagcgttac caccgcgtct gctggccgtt
     1621 cagaccgtgg ccggaaacgc tgtcaccgca gcaaggcgaa gtccagtgcc tgatcggcca
     1681 tctggccgaa agcgacctgc ataacggatt gatgtttatc gaacgggcgg aaggggtcct
     1741 gcacctgcgc gatctttacc tcgaggcagg cattgaatgc ccgacgcagc tggcgctggc
     1801 ggaacaactc acccgcgacg gctatcccgt cagccagtcg caaatcagcc ggatgctaca
     1861 gaccgtagac tggctgctgc cctgcatccc aaacgcgcta tatggcggtc ttacccgcaa
     1921 ggttatcgat cgcctgctgt cactacgcag cgcagcagaa caggtctggg taaagctgat
     1981 gccgctggaa cgccagcccg aatttgccga cctgttcagc accgccctcg ccatcttcaa
     2041 cggcgagccg gaagccgtgg taccgcaact ggtgcaggac gaactgctcg gtgagatgag
     2101 ctgtcgcagc ggcatgagct ggaatacgtt gctggcggaa ctgactgacg gtcagagcaa
     2161 gcggcaggtg ctgctggggc ctccggccga aaaggtcctc tggccgccac cgccggaagt
     2221 ggaaaaagca ccactaccag agcctgagcc accgactgaa cccgccgcgc ccgatgagcc
     2281 aaagccctca ccgctggtgc atacggcaca gtcgctgtgt gcctgctggg gactggacgc
     2341 ctgtatccag ccggacgaca gcgctccggc cggtttcacc ctcgcgcctt gccacgattt
     2401 tccccacccg gcggcacagc gctgctatca ggtgctggcg gccctgaatg gcgacaccag
     2461 tacgccgatg cccgccctcg aattgttgct gtcgccggac tacaacaacg ctcagatcca
     2521 gcaactgatg cagcttatta gcctgtgccg ccagcggcgg ctgcgggagg tgcgccatga
     2581 caccctcagt taaccaggca gtactcaccc acatcgtcca ggcgctgaag gaggggcaga
     2641 tccgctactg cgaatcgctc ggcttcagcc cgcaggagct gtgcgagctg acgcgcctta
     2701 ccgccgacga catcctgttt ctcagcaaca gcgcggtgca gttcatcacc acccacattg
     2761 atcacgagat gctgggccgg atgctcgcga ggatggagca ggagcggctg ttccagcagc
     2821 gtctggagga agcgctgtcg ctcggcgcgt ccatcgagtt cattaaccac tattttggcc
     2881 tttccacgcc ggaggtctgt gcccggcggc ggctgattgg cctgtttgtc cgccaggggc
     2941 gcaacaatgc cccggatgag caggtggaga cgctggtgtg gaacgagtgg cagaagacgg
     3001 gcgtgaaaaa gctcgactca cccgaagcgc taaccgccat gatggcaatg gcccgcgaac
     3061 acgatctgtc gctgacggtc atctggaacc tgctgcgcca gtggactcag gagaagctgc
     3121 tgcatgaaga gtgacgatcg gctgctgttt ctcggcaacc cgcagcagag cgtgccgaag
     3181 cgcctgctgc tggcgcgcaa cctctccccg cgcgacaagt tcgcgtggca actgctgcgc
     3241 ctgcatgccc gcgacgacgg cagcggcgtc ttcccctcct acaacgaact gcagggctgg
     3301 ctctctgacc agcctgaagg cgagaaggcc tcgcgcagta ccgtcagcaa tgcactgatg
     3361 atgctgcgga tcacccgctg gctgagcctg tgccagcggc tgcgcgacga gcaaagcggc
     3421 aggatcaccg gcaacctcta cgccctgcat gatgcgccac tgactcatgc cgatgcctgt
     3481 cggatggaca gtgaatacct gtcgctgctc accgcttgtt cccgccaccg caaccgacgc
     3541 atccgaaaca tcgctatcgg gctgctgctc accctgcaac aacctgtgga cggtggacga
     3601 acctgcactg agtctccatt gaagaccgcc cctggtctta atcgaggact cagtaaaaaa
     3661 tgcccgagtc ttcaattaag actcagtaat gtggcggggg ctggcgggca gccggagccg
     3721 cattgcgtac atactggtac agatttaaac aataaacatc aaaagcgtac gccagacctg
     3781 cactggcctg tggataatcc cttcagcgcc agcgagcgcc gcgccgcaga acgcgcgatg
     3841 cgcaatctgg atcccgctct ctgccagcaa gtgctggacg actgcctgtg gcggatcgcc
     3901 gcccacgata tccaccgccc cctcgcctat ctgctggcaa cgctggagaa agccagacgg
     3961 ggcgagttca atcagcttcg gcgcaggcgc agatagccct gattttatgc gcaccgcata
     4021 aagccggtgt accaactatc aatcaaacct cattcatcgg cgaaattaac tttctgcgga
     4081 ccagatgggt ttcccgtcgc ctgactcatt gtgcggaccg acactgcggg catctgaatc
     4141 catggagaat ttccgatgcc tggcacaacc ctgttcagcg atatccgcat caccctgcat
     4201 acccgcgtcg ccgcccggct gtggcaggcg catcccactg gcatgctgct ctgtctggcg
     4261 ttactcaggc gtctgctgcg cgccgaagag gccgacgatc cgtgggccgc ccactggctg
     4321 aagcaactgc gcatccgcct gaacctgatc gcccacctac tgaagcaaaa aaaccgtcgt
     4381 ctggaccatg ctttcgccag cctgccaggc gctatccaca ccacgcaggt cagtaacccg
     4441 tccccgaccg aattcatcct gccgctggcg ctattctcac cgcccggttc gcgcctgctg
     4501 caacagttga ttgattacga tctgctggtc cgccgcaccc tgcttgcctg gcatctcggc
     4561 cttatcaccc aggcggagaa gcgcgatttc attgccacca tcccacgcct gatgctccag
     4621 gtgttcagct tcgtgaaccg ctttcgcacc actggcgtga ctcgcgccga cgtgcgggcg
     4681 aacagtccgc tggcgcagtc gatggcgcat aagctcggaa atttgcccaa aaagatgctt
     4741 acggagatac agcgcgatgt tcaatgacga tgatgatgaa cgctccgccc gcaaaagctc
     4801 gctaaagggc cttttaaagg cctttaaggg taagggagag ctgcgcgcgc tggtacagga
     4861 attacaggcg ctgcgcttgc agctgcaaca aaatcagcca gtgctgaaac aggcggtgta
     4921 tgaactgccg gggcgtaatg taccgctgat tctgtgcagc aacccggcgc gctccggcgt
     4981 cagctatctg cgctggcgca atctggacaa caaccgttcg gggagcgtgg cgttgcaaga
     5041 ggccattcaa cagccggacg cgagcgatga actgcgcgac gcgctgatgg cgctcgaatg
     5101 gcggcggcat gtgctgaata cgcagctttc agtggtgatg tttatgctgc ggcggataca
     5161 accgttaacc accctggttt aaaattttgc gcaaagttaa accctgagtg tcactttatc
     5221 gattatttta aacttaactc atgcacctta aaacaggcac tgtgcgctta atgcacgttt
     5281 taaggtgcaa tttttacctt ctcctaccag tttcccctgt cgccttatcc ctccggcaca
     5341 ccatgctgaa ctccctttca ccggagacag cccatgcaac tttacctgtg cgaaaaaccc
     5401 tctcaggcac gcgacatcgc ccgaattctt gacgcccata accgcagcga cggctacctg
     5461 cataacgcca ccgtaaccat cacctggtgt ttcggccatc tgctgagcat ggccgcgccg
     5521 gaggattacg atccggcgct gaaacactgg cggacggaac agctgccgtt tattcccccg
     5581 gcatggcggt tggtgatccg cccggaggtg cataagcagt tccgtatcat cgaaaagctg
     5641 ctgaaacagg cggatgcagt ggtgattgcc accgacgccg atcgcgaagg cgaaaccatc
     5701 gcccgcgaaa ttctggaaca atgccgctgg cgcggtccgg tccagcggct gtggctgtcg
     5761 gcgctggatg aggccagtat ccacaaggcg ctcgccactc tgcgtcccgg cgacagcacc
     5821 tttccgctgt atcaggccgg actggcgcgc gcccgcaccg actggctggt cggcatcaac
     5881 atgacccgcg cctgcacgct aatgaaccgg cggcacaagg gcgtgctctc cgtgggccgg
     5941 gtgcagacgc cgaccctgcg tctggtggtg gagcgcgacc gggagatcgc ccgcttcacg
     6001 ccccgcccct ggtggcgggt ggatgcccgt ttgcatgcgg agggttgttc ctttctggcg
     6061 cagtggcagc cggatagtgc gtactgtgac gacgaaagcc gctgcatccg cgaatccgcc
     6121 gcccgtgagg ccacagcacg cctgcaacaa gccggaaccg cgctggtaat ggacgtgaca
     6181 acccggcggg agaaaacgcc gccaccgcag gccttcactc tcagcgccct gcagcaggcc
     6241 tgctcaaaac agtggggcat gggcgcacag gaggttctgg atatcgccca gctcctgtat
     6301 gaaacccata aagccaccac ctacccgcgc accgactgcg gctatctgcc gctctccatg
     6361 cacgatgaag caccacaagt tctggcggcg ctggtcgcca gcgacgcttc actccagccc
     6421 ctcgccacgc agcttaatct gcagcaacgc tcccggttgt gggacgacag caaaatcacc
     6481 gcccaccatg gcatcatccc gacccgtaaa acggtagatc tgacgcagat gaacgacagc
     6541 gagcgcaagg tctacggcct ggtacgcagc cattttctgg cgcagtttct gccagaccac
     6601 gaagtggata aaacggacct gcgcctgaac tgcgccgggg aaacgctgac tgcccgtggc
     6661 tcagtggtga ttgtcagcgg ctggcgacaa ctgtttttgc gcgaaatggc aacgcactca
     6721 ccggaagaaa cgcaggcact tcccgccctg caacagggac aaacctgcta ggtggaacag
     6781 gtggacgttc gcgcccgcca gacccagcca ccggagcact acaccgaagg gacgctgatt
     6841 gccgcgatga aaaatgccgc ccgttttgtc agcgatgaac gcctgaaaca gcggctgaaa
     6901 gaaaacgcgg gcatcggcac cgaagccacc cgcgccggga ttatccagac gctccttaag
     6961 cgcggctatc tgattaagca gcgtcgtttc ctgctggcaa cggataccgc cagcacgctg
     7021 attgacgccc tgccggaaac gctgaaagat ccaggcacca ctgcactgtg ggagcaaatg
     7081 ctggatgaca tcgcgacggg gaaaatggga ctggaggcgt tcctggtgca gcaacagcag
     7141 aacgtgacag agctgattaa gtcgttacgg aatgagtaaa taacctgttt cctgcatcac
     7201 acttttactt ttccctgatt gctcgttcaa attggcagtc tgatgatcct ctctgaggaa
     7261 tattcctcat ttttcggagt gttaagatgg ccaagaatac cggagacggt tatcgcaagg
     7321 gctctgttac agaccgcaca caggttcaga atccgcagaa tggcaactac accaaacgcg
     7381 atacaacgac aggtcgcttt atcgaccaga agcaggacgg cactcccttt aaaggtgtcg
     7441 ccaaagaagt cgatgaccgc cggaaataat atttagctaa ctttcagggc cttagttatt
     7501 aaggcctttt tgcattctgt acttccaact ttcacgccgt aaaaacaatt ccacctttcg
     7561 tttctcccta ccgaagattc aactaaacag gtcaacccca aaacccgcca tgcattcctg
     7621 aaacgcggag gcgcacagcg cggaatgccc gtactgccag taactcagag gccttgcagc
     7681 caatgagcca aacccgaagc ggggcgttcc cgcgcatggg gcgctccgca ccttcaacac
     7741 ggagatacca ccatgaacga tacccactac ttcaacctgt acaccagcgg tctgggctac
     7801 ctcaaccgga tccgggaagt gaccccgaaa aaaggcgaac cgttcctgtg ttgcgacatt
     7861 gccgcactac ggggcgaagc cagcaacgcg gagcacacct ggttcgactg ccgcgtcagc
     7921 ggcaaggaag cgcagaaact ggtgcgccgc tgtatcgacg cctgcgagca gaaacgcaag
     7981 gtgctgatcc agttccgcct cggcgatctg tacgccgaca cctttgtcta cagcaaaggc
     8041 gaccgtaaag gcgaaaccgg cgtcagcctg aaggcccatc tgctgcacat cgggctcatc
     8101 aaaattgacg gtgaagaggt ttaccgggca gagcgcgaag ctgaacctca gccagcgcca
     8161 gccgcactgc cagcagagcc tgtagcgcag ttctgacgca cagcattcac cctgccgggg
     8221 aaattccccg tcggggcgtt tccctggcct tttaccggag agacgcacga tgaataccgt
     8281 attgaactca cgcgaagaag cccttgaccg gggcaaactg attaaccttg atgacgaggc
     8341 gctggacgct ggcttgccac tggatctggc cattaccctt ccggcccaca agcactgtct
     8401 tgaagaccgg aatgacaacg ccatcaggga cttgctgaaa ggtgttgaaa tggcagcggt
     8461 agtggccttt atctcccacc aaccgccgca gcgcaccatt cgtatcgatt accgctcggt
     8521 gctgacgcaa aaaaccaacg gcagcacgca gcagcaaacc gtcagattga cgttacgcgt
     8581 tcatttttct gagcaaggca tccccatcgc cgccacgctg gggctgatgg gggaaagatt
     8641 cccttactga tctgattttg cgcccggaat aacgggcgct attttttgac gcagcaaaca
     8701 gttgtcggta gtccccagcc ctctcatccg gcacgctcct gtaaaagcac tgtggaggat
     8761 gcgatgaaac gactgctgac agctctgctc ctgtttattt tcaacggcgt ggccgcctcc
     8821 cctgcaacgc cagaagtcgt ccgctacgac cgctatctgc tggtcagcac gtcaccgcaa
     8881 caaccgccac tcgaacagct taccaccctg tccgttccgc ccggttttca cccgacagtc
     8941 ggtgaagcat tgcagtacct gctgcgcgac agcggctggt ctttgtgtct ctccgatgcg
     9001 cgccgggcgc agctttatgg cctcccgtta ccgttgaacc agtaccacac cggcccgctg
     9061 cgcctgaagg ccgccctgca actgctggca ggcccggcat ggcgaatgac cgcagacgaa
     9121 aaccgccgtg aagtctgttt taccagcgac cattccctcc cccagacggt ggcttaccgt
     9181 catcccgtgt ccctccaccc tttggggccg ttaacggccc cgttttttct cgccggaacc
     9241 gtacagcgcg gcgatatccg actggcagtg atcgtgccgc cggaggccct ttatctccat
     9301 caggtagtgt ggctgcgtat cggggagcag tggcacggct ggcggctggc gcggtttgat
     9361 gagcgccggg cggtattccg ccacggtaaa cgccagctga cgctgaaagt accggagtaa
     9421 gcgatgaaac gcctgattct cctgttactg ttcaccgcca gcacgtgggc gcaaccgctt
     9481 caggtgcaga ccagccagaa cgtgggttca gccctgcaac cactgaccac gcagcagcag
     9541 gccaccgtct ggggcatcac gccagaggag tggcagcgct atctcacgct gaaacagcag
     9601 gcgcgcggcg tctggtcgcc ggggctcgat ccactgacca cactcggcgt agaagccgac
     9661 actgaccacg aacgccagcg ctttgccgaa ctgctggtca ggcaggagta ccagcggctg
     9721 gagaaagaac tcgccttcca gcgtgcttat gatgccgcct ggcagcgcct gtatccgggc
     9781 ctgacgccgc tgaaaaccac ctctgccagt acttcccgcg tttcgctatt tgttaaagat
     9841 gactgcccgc cctgcgacag cctgctgcgt accctgctgg cgcaacagcg cccgctggat
     9901 atctggctgg ttgggagtga aggcgatgat aaccgggtgc gccgatgggc gatggcgcat
     9961 ggcatcgacg caaaactggt gggtgaacac agcatcacgc ttaaccacga cgcgggccgc
    10021 tggctcctgt tggggcagag caaaatcccg gtggcgctgg aacgtaaggg ggagcaatgg
    10081 cgcgaggtcc tgccctgatc ctgctgctac cctgtctggc gctggcacag ccgctgccag
    10141 aagcgtacct gcgcaccgcg gagcatttcc gcgtgccgcc ggagctgctg tgggccgtca
    10201 cacaaaccga aagcggcacg aacctgagcg gcagacatct tccgtggccg tggacgctca
    10261 atatcagcgg gaccggctac cgctatgcca gccgggagca ggcctgccag gcgctgctgg
    10321 ttgccatccg ccgcaccagc ccaaagcgca ttgacgtggg actcgggcaa atcaacatcg
    10381 gctggcacca ggcatatttt gactctccct gcgacgccct gtatcccctt caaaacctgt
    10441 cgctcaccgc gcaactgctg cgtcagcgct acgatgaacg tcccggaagc tggctcaatg
    10501 ccgccgcccg ctatcatcgt cccgctggcg gcgcgcctgc cgcgcgatac cgcaagctgg
    10561 tcagccgcca tctgcaacag gcctcaccgc catgagccgc tacgcgctgg aggcgctgct
    10621 gcgcccaccc gtggagtttt acagcaccgt catcgctggc tgtggcgcat taatctgcct
    10681 gacgcaggcc ccgctgctga tgctgacgcc gtggcaggcc cgcatgatgg ccagtgccct
    10741 gatgcttctg gccctgcggc gcggctggca gggatggcaa atcgtacgct accagcgtaa
    10801 cctcctgcgc ctgccgcgct acgcgctcac cagccggaaa atccccgtca gccgccagag
    10861 gctgtttctc ggtaaaggct ttcgctggca gcccatccac acacagcgtc tgtacgactg
    10921 cctgcaaccg gaagccgcac gctggctgcg ccccggatgg tgttaccgcc tggcaagaca
    10981 catcgaaaaa cgcagcgagc ataccctgcc cggccttgcc cgactgctca gcgccgatcg
    11041 ctggctgaac ccagtacgtc cgctgccacc cgtaggcggc aatccgtggt tgcatggggt
    11101 ggaactaaaa gagtgcgacg tcactctgcc gctcagtgaa cgccccggcc atacgctggt
    11161 gctcggcacc acccgcgttg gcaaaacccg gctggcggaa ctgcttgcca ctcaggacat
    11221 ccgccgtggc gaggtggtga tcgtttttga tccgaaaggc gacgcggatc tgctgaagcg
    11281 tatgtggaca gaagcgcagc gcgccggacg cggcgaccgc tttatcctgt tccatctggc
    11341 gtacccggag ttctccgccc gctacaacgc tatcgggcga tttggccgca tttcggaggt
    11401 ggcgtctcgc ctcactggtc agctttccgg cgaaggcaac tcggcggcct ttcgcgagtt
    11461 cgcctggcgc tttgtcaaca tcatcgcccg cgccctggtg gcgctcggcc agcgccccga
    11521 ctatcagtta atttcccgcc acgtcgtcaa tatcgacgcc ctgtttatcg actatgcccg
    11581 catcgtgctg ccgcgccaca acacgcggtc atgggaagtg gtggtgcaac tggcggagcg
    11641 cgttaacgac aaaaacacgc cgcgccacat gctgggccgc gatcctcacg tggtggcgct
    11701 ggagcagtac ctcaacgcgg aaaaattata cgatccgatc ctcgatggcc tgctctcggc
    11761 ggtgcgctat gacaaaacct atttcgacaa gattgtcgcc tcactgctgc cactgctgga
    11821 aaagctgacc accggcacgc ttgccgccct gctggccccg gattaccacg atctggacga
    11881 cgcgcgtccg atcctcgact ggcaacaggc gatccgccag aaggcggtag tctatgtcgg
    11941 gttggacgcg ctctccgatt cggtggtcgc tgccgccgtc ggcaattcga tgtttgccga
    12001 tctggtgagc gtcgccgggc atctgtataa atttggcctc aatgatggac tgcccggcaa
    12061 tagcgaaaaa ccgccgccta tcaatctgca ctgcgatgag tttaatgaac tgatgggcga
    12121 ggagtttatt cccctggtca acaagggcgg cggtgcgggc attcaggtca ccgcctacac
    12181 gcagacgttg agtgatattc aggcccgtat cggcagcgca ccaaagacag gccaggtggt
    12241 gggtaacttc aacaacctga ttatgctgcg ggtacgcgaa accgccaccg ccgaactgct
    12301 gacgaagcaa ctgccacagg tggaagtgcg cagccgccag gtcagttcag gtgtcaccga
    12361 cacgccgggc ggcatcaacg gctttaccag caacacctgc gagcaggtga ccacccgcaa
    12421 tgtaccgctg ctggataccg ccgcgctgat ccagctcccc aaaggccagg cttttgccct
    12481 gctggaaggc gggcagttgt ggaaaattcg tctgccgctg cccgtagcgg ataagcaatg
    12541 cccactacca gattctgtcg cgcaactggc ggcgtggatg caggcgcgcc agcaggggct
    12601 aacggaatga gtgagctgcg accgcctccg tcacgcccac cacagggacc gattggctgg
    12661 ctattgtcac tggccggacg gattattggg ctggttatcg gtacgctggt gctgcgagtg
    12721 gtgctggaac tagtggggct atatttctgg tggccgcagg aagggagcag gcatgttttt
    12781 caggtgatga ggcaggagaa ggtggaactg attattgcgg tacagccaca tcctttgggg
    12841 aaagagatac gagtactgct ggagcatgga ataacccgcg ttaatgatgt cagacataag
    12901 gccgtgagtc ggttgttcca cgaacccgtt accgtcctgg cttataccat gatcagcttt
    12961 gcgctgcgcc tgacgtggct ggttatcatg ttgccactgc tgtgcctcag tgctgtaacc
    13021 gggctgacag aaggcctggt gcaccgggat ttaagacggt ttggctccgg gctggaatcc
    13081 atttttctcc accgttatgt catcaggtgc ggcagttcag tcaccaccac gctttgggtg
    13141 gtttatctgg cgcagccatt ttctctgccc gcgatgctgg ttttacttcc tgccgcctta
    13201 tggtccggga tagcggtatg gatggcagca ggaagcttta aacgttggtt ataagttgct
    13261 caaaaaatcg cacctcacta caatgtatac aatgtgtaca tgtgtatgag gccaccaata
    13321 tgggcgtaat gtcagtccgt ttaaacaaca atctttccac gcaactggaa gcattatcaa
    13381 aagcgaccgg acgctcaaag tcctggctgg caaatcaggc aattgaagat tatgttgccc
    13441 gggaggcctg gcaagttgca gagattgagc aggccattca ggaagctgat gccggtgatt
    13501 ttgccacttc agatgaggtg gataagctgt ttcaacgtct gggagtaaag ccagatggaa
    13561 attaagtggc tacgtaaagc cattgccaat ctcgaagctg agttccggta tattgctgaa
    13621 gacgatccgc aggctgcgga acggtttgtg aacgaagtct ggcgacttac gcaactgctc
    13681 aaagagcaac ccgctatggg ccgggaagga agggtacctg aaacacgtga gctcattctt
    13741 cataattacc cttatatcat tccctatcga gtacgaaata atataattca ggttttacgc
    13801 gtgtttcata cccatcggcg cttacctgta aattggtgat aaaaaacaca agacaagaaa
//
LOCUS       DAZUCC010000132        13834 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZUCC010000132 DAZUCC010000000
VERSION     DAZUCC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.133"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1347
                     /gene="chbC"
                     /locus_tag="ACHY09_003340"
     CDS             <1..1347
                     /gene="chbC"
                     /locus_tag="ACHY09_003340"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFS3886271.1"
                     /translation="IASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLI
                     NNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMAL
                     AEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFT
                     FIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWTLNTWGTNFHQIIMDTI
                     STPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSV
                     EAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGI
                     FQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGL
                     GAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            1398..1748
                     /gene="chbA"
                     /locus_tag="ACHY09_003341"
     CDS             1398..1748
                     /gene="chbA"
                     /locus_tag="ACHY09_003341"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFS3886272.1"
                     /translation="MMDLDNIPDTQTEVEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKTMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1756..2598
                     /gene="chbR"
                     /locus_tag="ACHY09_003342"
     CDS             1756..2598
                     /gene="chbR"
                     /locus_tag="ACHY09_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFS3886273.1"
                     /translation="MMQPVINALEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2702..4054
                     /gene="celF"
                     /locus_tag="ACHY09_003343"
                     /gene_synonym="chbF"
     CDS             2702..4054
                     /gene="celF"
                     /locus_tag="ACHY09_003343"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS3886274.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            4067..4825
                     /gene="chbG"
                     /locus_tag="ACHY09_003344"
     CDS             4067..4825
                     /gene="chbG"
                     /locus_tag="ACHY09_003344"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFS3886275.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSLDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGCYRDV"
     gene            complement(5083..7344)
                     /gene="katE"
                     /locus_tag="ACHY09_003345"
     CDS             complement(5083..7344)
                     /gene="katE"
                     /locus_tag="ACHY09_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFS3886276.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            7527..7790
                     /gene="cedA"
                     /locus_tag="ACHY09_003346"
     CDS             7527..7790
                     /gene="cedA"
                     /locus_tag="ACHY09_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFS3886277.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(7837..9228)
                     /gene="tcyP"
                     /locus_tag="ACHY09_003347"
     CDS             complement(7837..9228)
                     /gene="tcyP"
                     /locus_tag="ACHY09_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFS3886278.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(9361..9951)
                     /gene="ydjM"
                     /locus_tag="ACHY09_003348"
     CDS             complement(9361..9951)
                     /gene="ydjM"
                     /locus_tag="ACHY09_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS3886279.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(10115..10783)
                     /gene="hxpB"
                     /locus_tag="ACHY09_003349"
     CDS             complement(10115..10783)
                     /gene="hxpB"
                     /locus_tag="ACHY09_003349"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFS3886280.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYIDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            10930..11466
                     /gene="yniB"
                     /locus_tag="ACHY09_003350"
     CDS             10930..11466
                     /gene="yniB"
                     /locus_tag="ACHY09_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFS3886281.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFFLIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(11507..12367)
                     /gene="yniA"
                     /locus_tag="ACHY09_003351"
     CDS             complement(11507..12367)
                     /gene="yniA"
                     /locus_tag="ACHY09_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS3886282.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(12473..12763)
                     /gene="ghoS"
                     /locus_tag="ACHY09_003352"
     CDS             complement(12473..12763)
                     /gene="ghoS"
                     /locus_tag="ACHY09_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFS3886283.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            complement(12864..13793)
                     /gene="pfkB"
                     /locus_tag="ACHY09_003353"
     CDS             complement(12864..13793)
                     /gene="pfkB"
                     /locus_tag="ACHY09_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFS3886284.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPVGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         3467 a         3280 c         3538 g         3549 t
ORIGIN      
        1 attgcatcgc ttgaaaaggt actcctccct tttgcagtta aaataggaaa gcagccacac
       61 gttaatgcaa tcaaaaatgg ctttattcgc ttaatgccgt taacccttgc gggggccatg
      121 tttgtattaa ttaacaacgt ttttctaagc tttggggagg ggtcgttttt ttattcctta
      181 ggtattcgcc tggatgcctc aaccattgaa acacttaatg gtctgaaagg tattggcggc
      241 aacgtatata acggaacatt agggataatg tctttaatgg caccgttctt tattggcatg
      301 gcgctggcag aagagcgtaa agtcgatgcg ctggcggccg ggttgttatc cgttgcagca
      361 tttatgaccg tcaccccata tagtgtcggt gaggcctatg cggttggcgc aaactggtta
      421 ggtggggcga atatcatctc cgggattatt attggcctgg tggtggcaga aatgtttacc
      481 tttattgtcc gccgcaattg ggtcattaaa ttgcccgata gcgtacctgc ttcagtatcg
      541 cgttccttct cggctttaat tcccggcttt attattcttt ccgtgatggg gattattgcc
      601 tggacgttga atacctgggg caccaacttc catcagatta ttatggatac catctcaacc
      661 ccactggcat cgttgggtag cgtggtgggc tgggcctatg tgatctttgt tccactgctc
      721 tggttcttcg gtattcatgg tgcgctggcg ctgaccgcac tggacaacgg cattatgacg
      781 ccttgggcgc tggaaaatat cgcgacctat cagcaatatg gttccgtcga agcggcgctg
      841 gcagccggta agaccttcca tatctgggcc aagccgatgc tggactcctt tattttcctt
      901 gggggcagtg gtgcgacttt aggtctgatc ctggctatct ttatcgcctc tcgccgtgct
      961 gattatcgtc aggtggcaaa actggcgctg ccgtccggca tcttccagat taacgaaccg
     1021 attctgtttg gtctgccaat tatcatgaac ccggtgatgt ttatcccgtt tgtactggta
     1081 caaccgattc tggcggcaat caccctcgca gcgtactaca tgggcattat tcctccggtg
     1141 accaatattg caccgtggac catgccaacc ggtctgggag ccttctttaa caccaacggt
     1201 agcgtcgccg cattgctggt cgcactcttc aaccttggta tcgcaacgtt aatttatctg
     1261 ccctttgttg tggtggctaa caaagcacaa aacgcgattg ataaagaaga gagcgaagaa
     1321 gatatcgcta acgccctgaa attttaatcg atgccggtac gggtaatcgt gccggtaaga
     1381 atagagagga acgatgtatg atggatctcg ataatattcc cgatacgcaa acggaagttg
     1441 aagagctgga agaagtggtg atggggctta tcatcaactc cggacaagcg cgcagcctgg
     1501 cgtatgcggc actgaaacag gcgaagcagg gcgattttgc cgcagcaaaa accatgatgg
     1561 atcagtcacg gatggcattg aatgaagcgc atctggtaca gacgaaactg attgaaggcg
     1621 atgcgggcga aggtaagatg aaagtgagtc tggtgctggt ccacgctcag gatcatttaa
     1681 tgacgtccat gcttgcgcgt gaactgatta ctgaattaat tgagcttcat gaaaaactga
     1741 aggcataagg agtcgatgat gcagccagtg attaacgcgc tggaaattgc cactgcccga
     1801 gaacagcagt tgtttaatgg caaaaatttc catgtgttta tctacaataa aactgagagt
     1861 atcagcggac tgcatcagca cgactattat gaatttactc tggtattaac cgggcgttat
     1921 ttccaggaga ttaacggtaa gcgcgtgtta ctggaacggg gcgattttgt ttttattccg
     1981 ttaggttcgc accatcaaag tttttatgaa tttggtgcca cgcgtatatt gaacgttggg
     2041 atcagtaaac gcttttttga gcagcattac ctgccgttgt tgccttattg ctttgtcgct
     2101 tcgcaggtat accggaccaa taacgctttt ctcacctatg tggaaacagt gatttcttca
     2161 ttgaatttcc gcgaaacagg gctggaagag tttgttgaga tggttacttt ttatgtcatt
     2221 aaccgtttac gtcattaccg cgaagaacag gtgattgatg atataccgca gtggctgaaa
     2281 agtacggtag aaaagatgca tgataaagag cagtttagtg aatcggcgct ggagaatatg
     2341 gtgacgttgt cagccaaatc acaggaatat ttgacgcgag cgactcaacg atactatggt
     2401 aaaacgccaa tgcagattat taatgaaatc cgtattaatt ttgccaaaaa acaactggaa
     2461 atgaccaact attcagtgac ggatattgcg tttgaggccg gttatagtag cccgagcttg
     2521 tttattaaaa cgtttaagaa attaacatcc tttacgccta agagctatcg taagaaattg
     2581 actgaattta atcagtaagt cgttatacct gacaattcac atatctgtcc tgttgctgga
     2641 ttatttatgt ccggggggca gatatgccag atatcagtat tctgtactga agggagaaat
     2701 tatgagccag aaattaaaag tcgtcactat tggtggcggg agcagctata ccccggagtt
     2761 actggaagga tttattaagc gttatcacga attgccggtc agcgaattat ggctggtgga
     2821 tgtcgaaggt ggtaaagcga aactggatat tatttttgat ctctgccaac ggatgattga
     2881 taacgctggc gtcccgatga agctttataa aacgctggat cgccgcgaag cattgaaaga
     2941 tgctgatttc gttactaccc aactgcgcgt tggccaatta ccggcgcgcg aactggatga
     3001 acgtattcca ttaagtcatg gttatcttgg tcaggaaact aacggtgcgg gcggtctgtt
     3061 taaaggtctg cgtaccattc cggtgatttt tgacatcgta aaagatgtcg aagaactatg
     3121 tccgaatgca tgggtgatta acttcactaa cccggcggga atggtcactg aagccgttta
     3181 tcgtcatacc ggatttaaac gctttatcgg cgtgtgtaat attccgatcg gcatgaagat
     3241 gtttattcgc gatgttctga tgctgaaaga cagcgatgat ttatctatcg atctgttcgg
     3301 cctcaaccat atggtgttca ttaaggatgt gctggtaaat ggcaaatcac gctttgccga
     3361 attgcttgat ggtgtggcat ccgggcagtt aaaagcatct ggcgttaaaa atattttcga
     3421 tctgccattt agcgaaggct taattcgttc tatgaatctg ttgccgtgtt cttatttgct
     3481 ttattacttc aagcaaaaag agatgctggc tattgaaatg ggcgaatact acaaaggcgg
     3541 cgcacgagcg caggtagtac agaaagtcga gaaacaactt tttgagctat ataaaaaccc
     3601 ggagttgaaa gttaagccga aagaactgga acagcgcggt ggggcttatt actctgatgc
     3661 agcgtgcgaa gtgatcaacg ctatctacaa cgacaagcaa gctgaacatt acgttaatat
     3721 cccgcatcat gggcatattg ataatattcc ggcagactgg gcggtagaaa tgacctgtac
     3781 gctggggcgc gatggcgcga cgccacatcc gcgcattacg catttcgatg ataaagtgat
     3841 ggggctgatt cacaccatta aaggcttcga gattgctgcc agcaacgccg cacttagcgg
     3901 agagtttaac gatgtgttac tggccctaaa ccttagtccg ttggtgcatt ccgatcgcga
     3961 tgctgagctg ctggcacgcg agatgattct ggcgcacgag aaatggttgc caaattttgc
     4021 cgactgcatc gcagagctta aaaaagcaca ttaaccgagg ctgattatgg aacgcttact
     4081 gattgttaat gccgatgatt ttggcttaag caaaggtcag aactacggca ttatcgaggc
     4141 ctgtcgcaat gggattgtca catcgacgac ggcgctggtg aatgggcagg ctattgacca
     4201 tgcggtgcag ttgagtcttg atgaaccgag tctggccata gggatgcact ttgtccttac
     4261 tatgggcaag ccactgacag ctatgccggg gttaactcgc gatggtgtgc tgggaaaatg
     4321 gatctggcag ttggcagaag aagatgcttt accgctggaa gaaattactc aggagcttgc
     4381 cagtcagtat ttgcgtttca ttgagttatt tggacgcaaa cctacgcatc ttgatagcca
     4441 tcatcatgtg catatgttcc cgcagatttt cccgattgtg gcaaagtttg cggctgaaga
     4501 ggggattgcg ttgcgcatcg accgtcagcc gctatctaac gatggtgatt taccggctaa
     4561 tctgcgcagt tcgcagggat tcagtagcgc gttttatggt gaagagatta gcgagacact
     4621 gttcctacaa gtgcttgatg actccagtca tcgcggcgag cgctctctgg aagtgatgtg
     4681 ccacccggcg tttatcgata atacgattcg ccagagtgct tattgcttcc cgcggttaac
     4741 ggagctggat gtgttgacat cagcgtcgtt gaaaggtgct atcgctcagc gtggttatcg
     4801 gttggggtgt tatcgggatg tgtaagtgag gatgtttgcc agatgcggcg tgaaccttgc
     4861 atccggcgat taattcctac aaaggcggcg taactgcgcc gcttgagact gctgacaaac
     4921 gcaaaactgc ctgatgcgct tcgcttatca ggcctacgtt tttcctgcaa tatattgaat
     4981 ttgcacagtt ttgtaggccg gataaggcgt tcacgccgca tccgacataa acaaagcgca
     5041 ctatgtaaat catttgaggc ggcgcaactg cgccgcctcc catcaggcag gaattttgtc
     5101 aatcttagga atacgtgacc acacgcggtg tgctgccatc agcgttaaca gttcatccat
     5161 aaaactaccg tcggcgctgt cagcttccac aatcccttct tcaccctggt cagcgacctt
     5221 gattgttgct ttaaacttgc gcgcgtctcc tgccagcgca atcggtttaa ggtgtttgta
     5281 ggcttccatc aggtagtagt tggcatcgcc gttgtcagcg atatccgcga tattgccgca
     5341 agggacaata accgcatcga ccgtcagcga aggtgcaccg gcaaaggtag cggctatagg
     5401 caacaccgta ccgtcatccg cagtcacttc acccattcgg gagtagagca gtttggcatg
     5461 aacgcctttg gccttcagcg ccttgagaat ggccagaagg tctgccgatc tcacttcatc
     5521 attaagtaaa atcgctacca cgcgaccttt cacatcaccg tcaggaatgg cgtacaaact
     5581 taaggatgga tcctttttca gaccgttgac gtccggaggc ggggtgatat tcagctggtc
     5641 gtccgtcagc tcgataccga gatttttcgc caccgcctgg gccagagtga gatcaatatg
     5701 tgccagctgg tcaacaacgc gctcacgaat atacggacga acgactttgc ttaactcaaa
     5761 actgaaacca tcgacaatat ggcgctgctc gaatggcgtc tgacttagcc agaacagacg
     5821 cggatgggaa taatattcgc caaacgatgg gctgcgctcg cgaactttat tgccttccac
     5881 gcgctcctgg tatgattcaa aaccgccgcg tttcggcccc ggcggtgttt cgcgcggcca
     5941 gttatcgttg atcgagttcg gttcataatt cgccgggtta gtgtcgatcc ccatacgatg
     6001 catgccgtca cgctggaaat tatggtaagg gcaggtcggg cggttaatcg gaatctcatg
     6061 gaaattcggc ccaccaagac gactgatttg tgtatcggta taggagaaca aacgtccctg
     6121 caacagggga tcgttggtga agtccagacc aggaacaata tgacctggat ggaatgctgc
     6181 ttgttcgttt tcggcaaaga agttatccgg attgcgattg agcaccatct tgccgacacg
     6241 ctgaacgggc accagttctt ccgggataag tttagttgga tcaagaaggt cgaagtcgaa
     6301 cttaaattca tcttcctcag gaatcaactg gaagcccagt tcgtattccg gaaaatcgcc
     6361 tgcttcgatg gcttcccaca actcgcggcg gtggaagtcc gggtcacgtc cggttagttt
     6421 ttgtgcttca tcccaaacga gtgaggcttt acctgccagt ggtttccagt ggaaacgtac
     6481 gaacgttgcc ttcccttcgg cattaatcag gcggaaggtg tgaataccga agccttccat
     6541 ggtgcggtaa ctgcgcggga tcccgcgatc cgacatcgcc cacatcacgt tgtgcagagt
     6601 ttcaggttgc agagaaacat aatcccagaa agtatcgtgg gcgctttgcc cttgtggaat
     6661 tgcccagtgc ggttctggtt ttaccgcatg aacaaaatcg gggaatttat gcgcatcctg
     6721 gataaagaag attggcgtgt tattgccaac gaggtcaaaa ataccctctt cggtatagaa
     6781 cttggtggca aagccacgga tatcacgcac ggtatcggca gagccagcac caccctgaac
     6841 ggtagagaaa cgtacaaata ctggggtgat tttgttcgga tctgagagga aatctgcttt
     6901 ggtgatatcg cttaagcttt tatatggctg gaaataaccg tgagctgctg atccgcgtgc
     6961 atgaacaata cgttccggga tgcgctcatg gtcaaagtga gtgattttct cgcgcagaat
     7021 aaaatcttcc agcagagttg gaccacggct accggcacgc agtgagtttt gatcgtcggc
     7081 gatgcgcacg ccctgattag tggtcagcgc ataattttca ctgcctttgc gtacgtcttc
     7141 cagagaatta agtttttcgt tacgcgtatc aggggctttc aggctccctg gggcggtagg
     7201 ttgtgcacca ggcggtgttg gttcagccgc tggacgatga gatccgtctt caggtgccag
     7261 tgagtccatc cccggtttcg cttcgctgga atcgtgtagt ggtgactggt gctgatgtgg
     7321 gttcttttcg ttatgttgcg acattgaact cgtctcctta aattattact gaaagggccg
     7381 ctgttttggt caaaaaaccc tctaactata gaccatcagc cagatcccgc ttcggagact
     7441 attctggaaa cggtgttatg aaatacacgc aaagcgggac gtacaggggc taaatcggct
     7501 aaactacagt ttttctgatt tttaatatgc gtttagtgaa gccagtaatg aagaaaccgc
     7561 tccgccagca aaaccgccag attattagct atgtcccacg cacggaaccc gcgccgccag
     7621 aacatgcgat aaagatggat tcgtttcgtg atgtctggat gctgcgtggc aaatatgttg
     7681 cgtttgtact gatgggagag tcatttctgc gctcaccggc gtttactgtg cctgaatccg
     7741 cccagcgttg ggcaaatcag attcgtcagg aaaatgaggt cgaagaataa aaaaacacct
     7801 gtcgggatga caggcgttta acgtaacaaa tcaggattaa cggtgtgcca gttcggcgtc
     7861 gtcttcgcta tccagaatag ctttatcggt ttgcttcagc cactggctgg tcagcgtgcc
     7921 agcggtcatc gagccactaa cgtttagcgc cgtacgaccc atgtcgataa gcggttcaac
     7981 ggagattaac aatgccacca gcgttaccgg caggcccatc gcaggcagca caatcagtgc
     8041 ggcgaaagtt gcaccaccac cgacaccggc aacgcctgcg gaactaacgg taacaatacc
     8101 gaccagcgtc gcaatccaca tcgggtccat cgggttaatg ccaaccgtag gcgcaaccat
     8161 caccgccagc atcgccggat acaaaccggc acagccgttc tgaccaatgg ttgcaccgaa
     8221 agaggcggcg aaactggcga tggattcagg aacaccgagt cgacgcgttt gtgcttccac
     8281 attcagtggg atagacgcag cactggaacg gctggtaaag gcaaacgtca gtactggcca
     8341 taccttacgg aagtacttca gcgggctcac gccattaata cccagcagaa ggccatgcac
     8401 tgcaaacata atcagaagac cgaggtagga cgcgacaacg aaacttccca gtttgatgat
     8461 gtcttgcagg ttagaacctg caaccacttt ggtcatcagc gccagaacgc cgtaaggggt
     8521 cagctgcatg accaggcgaa ccagtttcat cacccagctt tgcagggtat cgatagcggt
     8581 taagacgcgt tcacctttcg gcgcatcatc cttcagcagc ttcagcgcag ctacgccgag
     8641 gaatgcggca aaaattacca cgctgataat tgacgtcgga ttggctccgg tcaaatcggc
     8701 aaacgggttt ttcgggataa aggacaagac cagctgcgga acgctcaggt cagagacttt
     8761 accaacatag ttactttcga tggcgttcaa acgtgcagtt tctgcaccac cctgaaccag
     8821 accttcagcc gtcaaaccaa acaggttggt gaccagcaca ccgaccagcg ccgcaatcag
     8881 tgtggtaaac aaaagcgttc cgatggtcag aaaactgatt ttgcctaact gtgatgcgtt
     8941 atgcagacgg gcaaccgcgc tcagaataga ggcaaagact aacggcataa cgatcatttg
     9001 caggagttga acatagccgt taccaacaat gttaaaccac tgtacagaat ctttaagtac
     9061 ctggctgtca gaaccataaa tggtatgcag ggcgaggcca aaaaccacac ccatcaccag
     9121 acccaccagc acttttttcg ccagactcca ctgtttatgg cgggtctgag ccagcgcaaa
     9181 cagcagtaca acgaacacca cgatgttcgc aattaatgga aagttcatcc ccgttctcct
     9241 gatcttatta tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat taacagaact
     9301 gtgagctggc tcttatattc aaatggaatt gcttattcca gaaaggtgat ttttgccttt
     9361 ttagtattca acctgatgct taataagccg atgaaactgg atctgcaagg tattgatcat
     9421 ttgcgatgac caacggacag cgctgttttc gggtaatgaa tggggcatcc ataccgacca
     9481 gacaaataat gccatgcaga taaaacgttc cagttggttg cccttttggg gaaccaggat
     9541 aggcaagcgg aaacgccagc ggcatggcca gagcagggga acaccggcgg gtgtcagcat
     9601 atcggcaagt atgtggctca aataacccag caccattcct tgtagcgcat cagccggaat
     9661 gaaccagctt tcaggaacct taaggtaaaa ggttgccagc agcgcaaata ccgccagcag
     9721 actgtgggta aaaccacgat gcccaaaagc gcgggcgatt ggttttgata tccactttaa
     9781 tcgctgccca agaaacgact ttgggtgatc gatgtccggt aacaaacacg tcaggattgc
     9841 ggaagggaca atatgccacc agtcaccctg tgccagcacg ggcgtcagct cggcattttt
     9901 ggcaaatacc gcacaagcaa tagaaaagag aaggtgacct tccgccgtca tgataaaact
     9961 cactaaactg tcgatacgta cagtataggg tttttataca gtgcacggaa gtggtgacgg
    10021 tgtaactctt tctcttaaat tggcgattaa tccgtcagtt gaatgcaaat tcagataaag
    10081 aaccaggggc agtgtgatca ctgccccctg gtcattaacc gagaaggtct tttgcggtga
    10141 gttctgtcag cgatgagagt ttgacgtttg ctaatacaaa acgtggatca ttttgcgctt
    10201 ctggcgcagg aacgacgatg gaacgcatgc gggctgcttt agaggcgatc atgccattta
    10261 ccgaatcttc cagtgccacg caggtcagag ggtcaacccc cagttttgct gcgcagtcga
    10321 tatatacttg cggatgcggc ttgctgtaag gcaatttttc agccgaggcg agggcatcga
    10381 aactgtcgcg taaatcaaac atggtcaata ctttttccag catatgcagc ggtgacgcgg
    10441 aggccagccc caccaataaa ccttgttctt tgcataacgc aacggcttca cgcacacctg
    10501 gtaataatgg acgcgtctct tcaaccagtg aaatggcacg agcaataact tgttctacta
    10561 cttcctgacg gcttggccca ttccatggtt gccgggcgta ccaaagatcg accaccatat
    10621 cgatgcgtaa acctaaggtg tccggcagct cgttacgacg ggagatatcc acccccaggc
    10681 ttgccatcac atccagttcg gctcgatccc ataaaggttc tgagtcgata agtaatccat
    10741 ccatatcaaa aattgcagca agaatctgac gcggggttga cattacaacc tctcctttag
    10801 agcattatat gtaaggtatt aagccgtgct tatgattaag cgcctatcat atcgccttta
    10861 aagaatgggc gaaaacagat atctacaggt aaactaagtg aaacgacgcg tcttaatcaa
    10921 ggagaactca tgacgtatca acaagctgga cgcattgctg ttttgaaaag gattttgggt
    10981 tgggtgattt ttatacctgc attgatctct acattgattt ctttgcttaa atttatgaat
    11041 acccggcagg aaaaccagga aggcattaat gcggtcatgc tcgactttac tcatgtcatg
    11101 atcgatatga tgcaggcgaa tacgcctttt ttgaatctgt tctggtataa ctccccgacg
    11161 cccaatttta atggtggcgt gaacgttatg ttctgggtga ttttcttcct gatttttgtc
    11221 ggactggcgt tgcaggattc cggtgcccgg atgagtcgcc aggcgcgctt cttgcgggaa
    11281 ggtgttgaag atcaactcat tctggaaaaa gccaaagggg aagaggggct aacgcgtgaa
    11341 caaattgagt cacgtattgt tgtgccgcac catactattt tcctgcagtt tttctcgctg
    11401 tatatcctgc cggttatttg tattgctgca ggttatgtgt tcttttctct gctggggttt
    11461 atttaatcaa aaggccgcac ggagcggcca ttatcctcac cccatattat gctgctaata
    11521 atctatccag tgactgctga gcaataacca aatgctgacc gccaaataac cttgcacgat
    11581 ttaacagcgt gtagagttgg taaaccggtt gacgttcaag gaaatcggca ggtagcgggg
    11641 atactgactg atagccgtca tagatttgtg gcggctgttc agtatgcagc ggtaacatcg
    11701 ccaggtcgca ctctcggtca ccccagtagc aggccgggtc gaaaatgtac gggccatccg
    11761 gacccagcgc acagttgccg gaccataaat cgccgtgcaa cagagaaggc tgcggttgat
    11821 gcgaggccag acgctgctga atatgctcga cgatggcatc gatattgccg aaagcgatcc
    11881 ctttttctgc cgccagttcc agctgccagc caatccgttg ttcagcaaaa aaagttgacc
    11941 agcgacgttg ccaggtgttg ggctggggag ttgtggagag cgcgttatcg aaatcgaggc
    12001 caaattgcgg ttggtcgctc cattgatgta aacgcgcaat ttgctgacca agaataaatg
    12061 cgctatgcgc atccagcggg cgaggtggga gataatccat caccagaaaa ctgtagtcac
    12121 ggtcagcgcc aactgcccaa accttaggca cggtgacggt tttactacgc gacagtaact
    12181 ccagttggtc ggcttcggcg gtaaaaccgg gaagcagttc cctttcatca catttgacga
    12241 aaaagtcatg tcctgcatag cgcaaatgcc atgcggcatg gacttctccg ccaggcagtt
    12301 cattacgcag ttcgatttcg ccttcaccta actgctcgct caaaagacga ctgattgcct
    12361 gccacatgat gtctctccca tgttgtctgc cagatcataa ggttagcgca agaatgcggt
    12421 gtaaaaacac gagctaacgc acaccacaga ctgattattg cccaaaaacc atttattttc
    12481 tgttgctatt ccattcctcc caggtcgtga tggctatttc aggatcttta ccggttgcac
    12541 tttgggtaag ccccgaaaca agttctctaa tttcttcttc actttgggta ctgataagtc
    12601 caaaagtatt agtccccagc tcatggatat ttccatcatc atccgtcatg gtgagcaaaa
    12661 aaccgtcgcg agtcaggtaa ttattcaact cgttaatttc agtcaatgta tcttcgtgca
    12721 acatgacggt tatgacataa cggacaagat cgccactggc catagttcac ccctttgtta
    12781 tcacatgact tttgagcata gtcggagaaa cgcgttgccg acaggttggt ggtgattccc
    12841 ccaatgctgg gggaatgttt cagttagcgg gaaaggtaag cgtaaatttt ttgtgtatcg
    12901 tcatgggagc acaggcgtgt tccctgattg agtgtggctg cactccccgc agctacgcca
    12961 aaacggacca tctcttcaag agaggcattt tctgccagtt tcagtgtcat cgcgccgacc
    13021 atgctgtcac cagcgccaac ggtgctctgg cttttcaccg gtggtggcac aacctgaatg
    13081 cagttttcac tatcaacgcc cagcgcccct tgtggaccca gggaaacgac aactcgtttg
    13141 gccttgccgc tattaacgat ttcctgcgcg gctttgcgga catcgtctgg ctgggtgagt
    13201 tcgcgattca ccagcgcact gagttctttt tggttaggct taaccaactc gatgttacca
    13261 atcgccagtg ctgcacttaa cgcttcgcca gaactgtcga cgatacagcg gatcccttgg
    13321 ttttgcgcag cggaaatcag ttgggttaat ttttccagct tcacacctgg cggcaggctt
    13381 ccgcttatga ccaggatagc accggattca atttccagaa cttgctcttc aagctggcga
    13441 aactcatctt catttaatgc cgcgcctggc atgacaaaac gatactgctc accgcttgct
    13501 tccacatgta cgtgtaaatt ctgccgggtc cagtctttgg cttctacagt agcgacgggg
    13561 acattttcat ctgccaacag tgaaaccaga tgttcgccgg tcgcacctcc caccgggaag
    13621 atcgctgtag cactgcctcc aagatgggca atggcgcggg cgacgttgat gccgccgcct
    13681 ccgggttcga ataccggtgc ggtacagcgc agctttcctt cgggataaat ttgcggggta
    13741 attgttgcgc tatcgagaga gggcgcaagt gtcaacgtat agatacgtac catcatttcc
    13801 tcctataggc tgatttcagt ctggcaccga atca
//
LOCUS       DAZUCC010000133        13739 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZUCC010000133 DAZUCC010000000
VERSION     DAZUCC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.134"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1121
                     /gene="tssD"
                     /locus_tag="ACHY09_003354"
     CDS             <1..1121
                     /gene="tssD"
                     /locus_tag="ACHY09_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFS3886285.1"
                     /translation="YLTITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSLSNSIT
                     NTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYYNIQLR
                     GALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSHLAFPDNYNRLFIPRQKT
                     KDDNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNKGLLIA
                     GASIVAGVVLGRMRFPDLESFEHFGARGAVSGRPFKPDLAGGPIENLTIDGVTINREG
                     IAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYCNLGWE
                     TGEPNGLDGYELWNNAHTATLEDFKIKDTDLFHPDAKK"
     gene            1118..1375
                     /locus_tag="ACHY09_003355"
     CDS             1118..1375
                     /locus_tag="ACHY09_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS3886286.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            1450..1695
                     /locus_tag="ACHY09_003356"
     CDS             1450..1695
                     /locus_tag="ACHY09_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS3886287.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(1864..4125)
                     /gene="metE"
                     /locus_tag="ACHY09_003357"
     CDS             complement(1864..4125)
                     /gene="metE"
                     /locus_tag="ACHY09_003357"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS3886288.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4243..5196
                     /gene="metR"
                     /locus_tag="ACHY09_003358"
     CDS             4243..5196
                     /gene="metR"
                     /locus_tag="ACHY09_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFS3886289.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5084..5983)
                     /gene="bioP"
                     /locus_tag="ACHY09_003359"
     CDS             complement(5084..5983)
                     /gene="bioP"
                     /locus_tag="ACHY09_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFS3886290.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(6059..6859)
                     /gene="yigL"
                     /locus_tag="ACHY09_003360"
     CDS             complement(6059..6859)
                     /gene="yigL"
                     /locus_tag="ACHY09_003360"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFS3886291.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(6867..7889)
                     /gene="pldB"
                     /locus_tag="ACHY09_003361"
     CDS             complement(6867..7889)
                     /gene="pldB"
                     /locus_tag="ACHY09_003361"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFS3886292.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVIVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8000..8620
                     /gene="rhtB"
                     /locus_tag="ACHY09_003362"
     CDS             8000..8620
                     /gene="rhtB"
                     /locus_tag="ACHY09_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFS3886293.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKPL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8682..9302)
                     /gene="rhtC"
                     /locus_tag="ACHY09_003363"
     CDS             complement(8682..9302)
                     /gene="rhtC"
                     /locus_tag="ACHY09_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFS3886294.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9366..11195)
                     /gene="recQ"
                     /locus_tag="ACHY09_003364"
     CDS             complement(9366..11195)
                     /gene="recQ"
                     /locus_tag="ACHY09_003364"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS3886295.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENDVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPISASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11327..12196)
                     /gene="pldA"
                     /locus_tag="ACHY09_003365"
     CDS             complement(11327..12196)
                     /gene="pldA"
                     /locus_tag="ACHY09_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFS3886296.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITRHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12361..12828
                     /gene="yigI"
                     /locus_tag="ACHY09_003366"
     CDS             12361..12828
                     /gene="yigI"
                     /locus_tag="ACHY09_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFS3886297.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12880..>13739
                     /gene="rarD"
                     /locus_tag="ACHY09_003367"
     CDS             12880..>13739
                     /gene="rarD"
                     /locus_tag="ACHY09_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFS3886298.1"
                     /translation="MDTKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPKKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDA"
BASE COUNT         3432 a         3584 c         3420 g         3303 t
ORIGIN      
        1 tttacctgac cataaccgga gaacaacaag gatgtatttc ctctcgttgc ggaacgagcg
       61 catcaatagg taatcgctgg cagattggcc atgaagatga aatcttcgcg ttctcgctat
      121 ctaatagtat tacgaataca ggcaaagggt cacaattaca tggccttagt ttctgtaagt
      181 tgattgacaa aagctccccg ttacttatta acgctattaa caataacgag cagttgttca
      241 tggagtttga cttctatcgg atcaatagat ttggtcgatg ggagaaatat tacaacattc
      301 aactccgcgg cgcgctctta tcagcaatca atcacctgtt tactgagaac aatctggata
      361 cagaaaccat cactgtcagt tatgaatata tactctccag gcatctgatt gctaatactg
      421 aatttagtca tttggcattt cccgataact acaaccgtct gtttatacca cggcaaaaaa
      481 ctaaagatga taatggcctc aaaacactca atagtaaagc ggttggcaga ctgcttgctg
      541 ctggcggcat atacaatggg aatatcgaag gattcaggga aacagcaaac aagttgggag
      601 gagatgcacc tgctggttac gatcaagtta tggataacaa agggttactc atcgctggtg
      661 cttcaatagt tgctggagtt gttctgggaa gaatgagatt ccctgatttg gaatcatttg
      721 agcatttcgg agccagagga gcagtatcag gaagaccatt taagcccgac cttgccggtg
      781 gtccaataga aaacctgacg atagatggcg taacaatcaa tagggaaggt atcgctattg
      841 ttgaaaagca tatctccaga tttgatcatg atccagctaa tgatgtaatg atttcaaggc
      901 taaaaaaaat agcaaataaa gaattgcttc ctgaaaaata cgatctgaac tactatactc
      961 atgaatgccg cgagtatcag cgttattgta atttaggatg ggaaacaggt gaaccgaatg
     1021 gtttagatgg atatgaatta tggaataatg cacatactgc aactctagaa gactttaaaa
     1081 ttaaagatac tgacttattt catccggatg caaaaaaatg acgattacga tagttgaatt
     1141 taacgtgctg aaagttatgg ctgagaaaga tattgactgg agctggatgg tgctggatcg
     1201 tactttagct atacgaaata ttccaggatt tggcaacgta gcgaatatcg taacaaagct
     1261 ggttaatcat gggttggttg atatcgtaaa tggtgagggc aactctaaac cacgctacag
     1321 agtttctcaa tatggattga atcttatcaa agaacaacaa gacaacctct tctgatccag
     1381 ccattacagg agcatgattt acattgtgat ctttcaataa gatactcatc aattttggta
     1441 cagaatatcg tgatcactaa aaatgaactt aacgtgctga acgttatgac tgagaaagat
     1501 ataaactgga actggatgaa tctcgatcgc acactatcca taaggcaaat accaggattc
     1561 ggtaatgttg ttaatattgt taacaaatta gctaacgaag gtttggttat tattgaggat
     1621 agcgggcata agtctatgcc tcactaccac gttagtgaaa agggatacaa tctcgtgcaa
     1681 cgtgagaata aataacgtag cgttgattac tgaacggaat cactcaaaac tgtgttttgc
     1741 gaaaaaaacg attcggttat ttctactcct gaagatctgc ttcaatctgg cactcatgca
     1801 aaatggcttc cagtactgga agccattatc gtactgcaat ttacaatgac tcgttaatac
     1861 tcatcatccc cgacgcaaat tctgcgccgc ctgcaccatg ttcgccagtg ccgcgcgggt
     1921 ttctggccag ccgcgcgttt tcaggccaca gtccgggttt acccacagac gctctgccgg
     1981 aatgcgtttt gccgctttct tcagcagggc ttcaatccat tccacgctcg gtacgtttgg
     2041 cgagtgaatg tcatagacgc ctggaccaat ttcatttgga taatcaaact cttcaaacga
     2101 ctccagcaac tccatgtcgg aacgcgaggt ttcgatggtg atgacgtctg cgtccagcgc
     2161 cgcaatcgaa tccatgatgt cgttgaactc gcagtaacac atgtgagtgt ggatttgtgt
     2221 gtcatctttc gccacggcgg cgttgatacg gaaagcctcc acgccccact ggagataggc
     2281 atcccagtcg ctgcgacgca gcggtaaacc ttcgcgcagt gccggttcat caatctggat
     2341 gatgccaatt ccagcggctt ccaggtccgc cacttcatca cgcagcgcca gcgcaatttg
     2401 tttggcgatg gtttcacggc tgacatcttc acgcgggaat gaccagcaga gtatggttac
     2461 cggccccgtc agcatccctt tcaccggttt gtcggtcagc gattgtgcat atttcgccca
     2521 ctccacggta attggtgccg ggcggctaac gtcaccaatg acaatcggtg gcttcacgca
     2581 gcgggaaccg tagctctgta cccaaccgtt ttgcgtaaag acaaagccat ccagatgctc
     2641 gccaaagtat tccaccatgt cattacgctc ggcctcgcca tgtaccagca catccagtcc
     2701 caaacgttcc tgctcgacaa tggcctgctt gatatgttcc gcaatgcccg tgcggtagtt
     2761 gttggcgtcg agattaccct ttttgaaatc cagacgcagt gtacgaattt ccgtggtttg
     2821 cgggaaggaa ccaatcgtgg tagtcggcca cgcgggcagt ttaaaacgcg cacgctgggc
     2881 ttcagcacgc acttcataga cattcgcacg ctgactgtcc tgggcggtaa tggctgcaag
     2941 acgcttttct accgccggat tatggacacg ggtagagtga cgacgcgcct gaatcggagc
     3001 gctccattct gccagagccg ccgtatcacc actgttcagc gcatcgcgca gcagtgccag
     3061 ttcatggcat ttttgtaggg cgaaggcaaa ccagcttttc acttctgcat caagacgcgt
     3121 ttccacgctc aggtcgatgg ggctgtgcag caacgagcag gaagatgcca cccacaaatc
     3181 gcgtttgccg acaatgtcct taatttgcgc atatttctcg gtaagatcgg cgcgccagac
     3241 gttacgacca ttgatcagac ccgcagacag tagccagtca gaaggcagac gcttgtgcag
     3301 ttcagcaacg tcatctttac catgtacaag gtccacatgc agcccctgaa caggcagcgc
     3361 agtaatcgtg tcgagatttg gcgttacgcc ttcaaaatag gtagtcagca gcagtttcac
     3421 ctggccctgg agcgcgtcgt aagcgggttt gtatgcgtcc agccacgcct gcggtagttc
     3481 cagtaccagc gctggttcgt cgatctgtac ccactcaatg ccgcgttttg ccagttctgc
     3541 caacacttgc tgataaaccg gcagaatgtc gttcagcagg ctcaggcgat caaactgttc
     3601 acctttcact ttacccagcc acaggtaggt aaccggcccc agcagcacgg gcttgacctt
     3661 gtggcccagc gccagcgcct cgtccacttc ctccagcagc tgcgtccagg tcagtttgaa
     3721 ctgttggcct ttaacgaact ccggcaccat gtagtgatag ttggtgttaa accatttggt
     3781 catttccgct gccgccgcag gttcgccagt cggtgcacgt ccacgaccaa tacggaacag
     3841 ggtgtcgata tctaccgaac catctttgtt ctgatgacgt ggcggaacgt tacccagcag
     3901 cagactggtg gtcagtacat gatcgtacca ggcaaaatcg cccaccggca gcaggtcgat
     3961 acccgcctgc ttttgttgat cccagtgacg ggcacgtaat tcacgcccaa cagccagcag
     4021 ttcttcacgc gtggagttcc ccgcccaata actctcttgc gcttttttca gctcgcgacg
     4081 caggccaacg cgagggaaac cgagggtgtg attaagaatt gtcattatat gccccttatg
     4141 gaatttttag tatttagccg tccagatgtt tacacatcca taattggcgg ttactgtata
     4201 ttcctcaagc gcaaattatt catgccgaag tgaaggactt tcatgatcga agtaaaacac
     4261 ctgaaaacgc tacaagcgtt gcggaactgc ggctcactcg cagccgctgc ggcgacgttg
     4321 catcaaacgc aatccgccct gtctcaccag tttagcgatc tggaacaacg ccttggcttc
     4381 cggctatttg tgcgtaaaag ccagccgctt cggtttacac cgcagggaga aatcctgttg
     4441 caactggcga atcaggtgct gcctcaaatc agccaggcac tgcaagcctg caatgaaccg
     4501 cagcagacgc gcctgcgcat tgccattgag tgccatagct gtattcagtg gctgacgccc
     4561 gcgttagaaa atttccataa gaactggccg caggtggaga tggattttaa atcgggcgtg
     4621 acgtttgacc cgcagcccgc gttgcagcag ggtgagctgg atctggtgat gacctccgat
     4681 attctgccgc gcagtggcct gcattattcg ccgatgttcg attatgaagt gcgtctggtg
     4741 ttggcacctg accatccact ggcggcgaaa acgcgaatca cgccggaaga cctcgccagc
     4801 gaaacgctgt tgatttatcc ggtgcagcgt agccgactgg atgtctggcg gcattttctt
     4861 cagcccgcag gcgttagccc gtcactgaaa agcgtcgata acaccttgtt gttgattcag
     4921 atggttgccg cgcggatggg catcgctgcg ctaccgcatt gggtggtgga gagttttgaa
     4981 cgccagggtc tggtggtcac caaaaccctg ggcgaaggct tgtggagccg actgtacgcc
     5041 gccgtgcgcg atggcgagca gcgtcagccg attacagaag cgtttattcg ttcagcgcgc
     5101 aatcacgcct gcgatcatct gccgtttgtg aagagcgcgg agcgacccac ttacgatgca
     5161 cccacagtga ggccaggatc accagcgcgc ctgtaataaa cgttggccag tgcggctgtt
     5221 ggtgccagat agccaggtta accaacagcc ctgccggaac gtgcatatta ttcataatgc
     5281 ccagcgttcc ggcgtccacc tgagtcgcgc cgtagttcca cataaagtag ccaataccgg
     5341 aagccaccac gccaagaaac accagaatgc cccattgcag cgtggtttgc ggcattttct
     5401 gcgcatttcc cagcaaaaac catgcaatca ccgccaccag gaacgcacca agatagaacc
     5461 acgcaaaggc gttatgctgt ggcatcggac gagtttccat caggcgtttg taacccacca
     5521 tgccgatggc aaaagtgata ttggagagtt gcaccagcag caggccagtc cagaagtggt
     5581 cggtgacctg atcatagcga ataattccgg caccaatcac cgccaacaag gcgctaaaag
     5641 catagcccca gcgcagacgg cgcttactca tgatgtcata aatcagcgtg atgtagagcg
     5701 gagtcagtac ggtgaacagc aacagttcgg agaccgtcag gtagagatag gcgcgaaaac
     5761 tcagcatata catcacaccg agctgcatcg cgcccaccag catatacagg ccaaccgttt
     5821 tcaggctatt gccacgggta cgcagaaacg gcagaaacac gagtgccgcc aggccaacgc
     5881 gcaccagcac tgcaaaatag ctatcgacgt gccccgcaag gtactcgcca taaaagctaa
     5941 aggagaaggc ccacagaatc gtggtgatga taagtagcgc cacaatggaa aactctttga
     6001 ttaacgagta tccattgtag cgaagtatca ggttgacaac tgaccaaata aagaacgatt
     6061 acgataaata gagtttacgc agataatgcg gcaccgcgtc ttcggcatta gtaccaatca
     6121 cttccagctc cggatgcaga tctttcagac gctggtgcgc actgcccata atgcagcctt
     6181 tacctgccat cgacagcatt tcggcgtcgt tcatcccgtc accaaaggca atacaatctt
     6241 tcagactgta gcccagtttc ttcgccaccg cttccagggc atggcctttt gaaacgccgc
     6301 ccgccatcac ttccagacaa gttagggtag agaagctgac gttgacgcga tcgccccaac
     6361 gagcgttgat tgcctgctcc agcggcagca gttgctcatg agaatcgcag gtgaagaaca
     6421 ctttgctgac gccttccggc tccagtaatc ctggctcata cagcgcatat ttgaacactg
     6481 cttctttaaa aaagcgcatc tcttccgggc ggtggcgatt cataaaccat tcgtcgtcgc
     6541 gataaacgtt agtaatgatg tccgggttgt cgttgaccac gccaaacaga tcgctggcaa
     6601 tgtcgcgatc caggttatga gcaaaaatca gattgccatc cagatcgtga acacgcgcgc
     6661 cattggaggt aatcatgtaa gacttaatct ccagattatc gcgaatttgc cctacatcaa
     6721 cgtggtggcg accggtcgca aacacaaagt taatgccgcg cgcggtgagc agcttcagag
     6781 tttctttggc gtaaggggat aacgtatggt cgggagaaag taacgtgcca tctaaatcag
     6841 acgcaacaac ctggtacata agaaatttaa acctctgtag agcggtttcc gctgggtgag
     6901 ttatgcctgt tgaaaaaatc aacaatggcg tggagcgcga ctgagcgcat tgcgtccttt
     6961 tcaaaaagga tctcatggta cgcaccttta attaccagcg gtcgtccccc ttcgacagga
     7021 tggcccgcag cggtgcggag ttcacaaaaa cggtcgtgca tgcggttatc caccacgcgt
     7081 tcctcttcag cctgcaagag aagcgttggc gtggcgtcat cacccgcacc ggctaacacc
     7141 tgttcgccag ccagaatact ttcgcgtacc caatggtagg tcggcccacc gacgcgaatc
     7201 gttgggtcat cagcataaaa gcgtaagtta cgtcgatatc gctgtctgct gtgggtcagt
     7261 acgttgatag caaacggcaa cgcgcgccag cgcccggtgc ctattgcata gccatcacgg
     7321 aaacgtggat gcgcttcggc ccagttaagg atctgccgcg ccataaatga cggcatacga
     7381 atcacgatcc caaacattgg cgcagttagc gcaatggcgt cacatacacc tggatggcgt
     7441 tgcagaaata acgtggagat cgcaccgccc atcgaatgtg ccagtatata gcgcttacgc
     7501 cacggaccag gctggacctc ctgttgccag aatgccgcca ggtcatcgac ataatcatta
     7561 aagcgattaa catgcccaag atgcggatcg gctaacaggc gaccggaacg tccctgcccg
     7621 cgatggtcga tgattaagac atcaaacccc aaatggaaca ggtcgtaggc cagttccgca
     7681 tattttacgt agctctcaat acgccccggg cagatgacta tcacccggtc atggtgctgt
     7741 gcgcgaaaac ggacaaagcg caccggaatg tcatctacac cagtaaactc tgcttcatca
     7801 cgctgacgcc agaaatcagt cagcggtccc atggtaaaag cagcaaacgc gttttctctt
     7861 gtttcccagt ctttttgctg ctgaaacatc gggtaatctg cctcttaaac cacgtaaaat
     7921 cgttttttta agcgtgcctg acacaacgct tcgacagtag cgtattgtgg cacaaaaata
     7981 gacacaccgg gagttcatca tgaccttaga atggtggttt gcctacctgc tgacatcgat
     8041 cattttaagc ctgtcgccag gctctggtgc aatcaacacc atgaccacct cgctcaacca
     8101 cggttatcgc ggcgcggtgg cgtctattgc tgggcttcag accggactgg cgattcatat
     8161 tgtgctggtt ggcgtggggt tggggacgct attttcccgc tcagtgattg cgtttgaagt
     8221 gttgaagtgg gcaggcgcag cttacttgat ttggcttggg atccagcagt ggcgcgccgc
     8281 tggcgcaatt gatcttaaac cgctggcctc tactcaatcg cgtcgacatt tgttccagcg
     8341 cgcagttttt gtcaatctca ccaatcccaa aagtattgtg tttctggcgg cgctatttcc
     8401 gcaattcatc atgccgcagc agccacaact gatgcagtat atcgtgctcg gcgtcaccac
     8461 tattgtggtc gatattattg tgatgatcgg ttacgccaca cttgctcaac ggattgcgtt
     8521 atggataaaa gggccgaaac agatgaaggc attgaataag atttttggct cgctgtttat
     8581 gctggtagga gcgttgttgg catcggcgag gcatgcgtga aaaataatgt cggatgcggc
     8641 ataaacgcct tatccgactt actctgatga agcgtttggc atcaacgaga aataatcaaa
     8701 tgaatgccaa atccggcaaa taacgccccg gcaaaaccat caatccactt cgccagacgt
     8761 tgataaccac ggcgcatttg cggtagagca aacaggctgg caaccaccgt aaaccatgcc
     8821 agtgtttcga taataatcaa cgcgaaaatg ccccagcgct cagtggtgcc gacgttatcg
     8881 ccgacaaaca gcgagaatac cgaaccaaag tagataattg ctttcggatt ggcgagattg
     8941 gtcagtaagc ctttcaggaa actgcgcccg ctttttgcca gttctacctg tggcgcaggc
     9001 gcagaaacga cctctttttt cagtgcgcta cgcagcatct gataacccat ccagcagaga
     9061 tacaggccac cgcccaccat aatcagcgta tgcagccagg ccattttttc gataatcaaa
     9121 tgcaggccaa gcaacgcgat ccctgcccag accattacgc cgcaggtaat gcccagcacg
     9181 cccatcatcg cctctttacg ggaacgactg acagcggtct gagaaacaaa gaagaaatcg
     9241 ggaccggggc tcataagcgc cacaatgtgc accatggcaa cggtgaggaa taacatcaac
     9301 attcaattga ctcgcggaga aatcgtttac ggagtcttca tcctggcact tttttatgct
     9361 gctgactact cttcgtcatc gccatcgaca tgcgcacgaa ttagcgccat aaacggtttg
     9421 ccaaagcgtt ccagcttgcg catcccaacg ccgttaacgc tgagcatttc gctggcggag
     9481 atcggcatct gttccgccat ctcaatcaag gtcgcatcgt taaacaccac atacggcggg
     9541 acattacttt catcggcaat cgatttacgc agtttgcgta atttggcgaa cagtttgcga
     9601 tcgtaattgc caccgaaaga tttctgcatt gctttcggtt tgagcgccac aatacgcggc
     9661 acggcaagtt gtaaagagga ttcgccgcgc aacaccgggc gtgcggcttc tgtcagttgt
     9721 aatgccgaat gctgggcgat gttttgcgtc accagaccga gatgaatcaa ctggcggatt
     9781 acgctcaccc agtgctcatg acttttatcg cggcccatgc cgtagacttt cagtttgtca
     9841 tgaccatagt cgcggattcg ctggttattg gcgccgcgaa tcacttccac tacatagccc
     9901 atcccaaaac gctgattcac acgaccaatg gtggaaagcg caatctgcgc atcggttgaa
     9961 ccatcgtatt gtttcggcgg atcgaggcag atatcgcagt tcccgcacgg ctcctgccgc
    10021 ccttcgccaa agtagttcag cagcaccaga cgacggcaag tttgcgcttc ggcaaaagcg
    10081 cccatcgcat tgagtttatg gcgctcgata tcctgcaact gcccctgcgg cttctcttcc
    10141 agacaacggc gcagccacgc catatcagcc ggatcgtaaa acagcatcgc ttccgcgggc
    10201 aggccatcac gcccggcgcg accggtttcc tgataatagg attcgatatt gcgtggaata
    10261 tcaaagtgaa ccacgaagcg gacgttaggc ttgttgatgc ccatgccgaa cgctaccgtc
    10321 gccaccacaa tttgcaggtc atcacgctgg aatttttcct gcacgtccgc acggacatcg
    10381 ttttcaagcc cggcgtgata agccgctgcg ctgataccct tgctttgcag acgcgcggcg
    10441 gtgtcttcta ctttcgcacg actattacag taaataatcc ccgacttacc gcgttgttcc
    10501 tgtacataac gcatcaactg atcgagcggt ttgaatttct ccatcagcat gtagcgaata
    10561 ttcggacggt caaaactgct aatttgaatc agcggatcgt tcagccccag caggcgcacg
    10621 atatcctgcc gcgtggtgtc gtcggcggtg gcggttagcg ccataaacgg cagcgtcggg
    10681 aaccgctggc gcaactgacc gagcgcggca tattccgggc ggaaatcgtg gccccattgg
    10741 gagatacagt gcgcttcatc aacggctaat aacaccggat tccagtgcgc cagatgctca
    10801 agaaagttat ccagcatcag gcgttccggg gcgatataaa gcagacgaat ttgcccggtg
    10861 cgacagcctg tcatcacttc aagctgctgc tcgcgggttt gcgtcgagtt aaggcacgcc
    10921 gccgccacac cgttggcctg tagctgatcc acctgatctt tcatcaacga aatcagtggt
    10981 gaaacaacca cggtaagacc gtttagcagt aaggcaggaa tttgatagca aagggatttt
    11041 ccgccaccag tgggcatgac gaccaggcaa tcgcgaccgg aaagtacggt gtcgataatc
    11101 tcttcctggc cggggcgaaa ctgttggtag ccaaaggttt cttgtaaaac ctgtttagct
    11161 ccggactcca gattcaacac ttccgcctgc gccacattaa ccccgattgc ctgaaataaa
    11221 aacaggcgct attttcagcg cctgcgcgag aaactgcaat gcttttagtc tctgagaggg
    11281 taatcctgga tactggattc cagttttgcc tttcaatcaa ctcacattag aacaagtcgt
    11341 tcagcataac ccccacaccc acacgggtct ggttaaagtt atagtcgatg agcgattcgc
    11401 cataaccgct gtaaacctgg gtataaaggc gcacatgcct ggtgatcggg taacttaagc
    11461 ctaactccgc accaccgtaa ccggtgttcc agttgtactg tcctttcgca ctcagcaccg
    11521 catcacccag gtgatagccg attttaagct ggtagtaacc catatatttg gtgatatctg
    11581 ggttatcgtc agtattcccc accacatacc acggcttcac ctctaccagc cagttaccgt
    11641 tttctgccat cagacgggta taaaggcggt tccagctgcg ggaagtcggg tctgaacgcc
    11701 cgttagagtc gtggttataa cccatctcca catcgcgcag cgtccagcca gcaaagcggt
    11761 aatcggtggc aaaaccaagg aacaattgcg gttcgtagtt ggtttcacga aacggtgaag
    11821 actcttcgct attggacagt tgccaccagg atttttgcgt gtaagaagcc cccaacaccg
    11881 agtttggccc caatattccg cgccacagcg gaaatgccag gctcaactgg aatttcactt
    11941 catccttacg cgcgttttcc gcccagtcat aactggcgat cgcttctttg ttcagatcgc
    12001 tggtttgggt gtagatgagg tagttggtgt cataaggata gagcgtgaaa ggattgtcat
    12061 gctcctgcag catattcgca ataatactgc cacgcaccgc aggcgcgtca tgcacctctt
    12121 ttaccgttgc ctcttgcgca tacactgcca ttggcaacat aaacaccggc aacaaccagc
    12181 cctgcagagt ccgcatcggt cgtgttctcc gtaacgaatt attgattaga tgaatatttt
    12241 tctgcgggct attttacata cttaactcct ttgccgttag ttatcccttc ctaaagtaga
    12301 aaaccacaat taagaagcat aaaatcaaca tttcattaac caatgaggtg taaggatgat
    12361 atgtctgccg tactgaccgc tgaacaagcc ctgaaattag tgggtgagat gtttgtttat
    12421 cacatgccat ttaaccgcgc attggggatg gaactggagc gttacgaaaa agagttcgca
    12481 cagctggcct ttaaaaatca gccaatgatg gtgggtaact gggcgcaaag cattttgcac
    12541 ggcggggtca ttgcgtcggc gctggatgtc gccgcaggtc tggtgtgcgt gggaagtacc
    12601 ttaacccgcc acgaaaccat cagtgaagat gaactacgcc agcggctatc gcggatgggg
    12661 accattgatc ttcgcgtcga ttatctgcgc ccaggcaggg gcgagcgttt tactgctacc
    12721 agtagtctgt tgcgtgcagg caataaagtc gccgttgccc gcgttgaatt acacaatgaa
    12781 gaacagcttt atatagccag tgccaccgcc acctatatgg taggttgata acgtaaaagc
    12841 aggtaaactg cttttaccaa taaaaaatga gttttgccaa tggacaccaa acaaacgcgg
    12901 cagggcgtat tactcgctct tgccgcttat tttatttggg gtattgcgcc agcgtacttc
    12961 aagctgattt actacgtgcc cgccgatgag atcctgacgc atcgcgtgat ctggtcgttt
    13021 ttctttatgg tggtgctgat gagcatttgc cgccagtggt cctatttaaa aacgctgatt
    13081 cagacaccaa agaaaatctt catgctggca gtctctgccg tgctgattgg tggcaactgg
    13141 ctactgttta tctgggcggt gaacaatcac catatgctgg aagcgagcct tggttacttt
    13201 attaacccgc tggtgaacat tgtgctgggg atgattttcc tcggcgagcg attccgccgg
    13261 atgcaatggc tggcggtgat tctggcgata tgtggtgtat tggtccagct ctggactttt
    13321 ggttcgctgc caatcatcgc gctggggctg gcgtttagtt ttgccttcta cggcctggta
    13381 cgcaagaaaa ttgccgttga agcgcaaacc ggcatgttaa tcgaaaccat gtggctgctg
    13441 cccgtggcgg cgatttacct gtttgctatt gccgacagct caaccagcca tatggggcaa
    13501 aacccgatgt cgctgaattt actgctgatc gccgccggta ttgtcaccac cgtgccactg
    13561 ttatgtttta ccgcagcagc tacacgctta cgactctcaa cattaggctt tttccagtac
    13621 attgggccga cgctaatgtt cctgttggca gtgacgtttt atggtgaaaa accgggtgcc
    13681 gataagatgg tgactttcac ctttatttgg gtggcgctgg caatttttgt gatggatgc
//
LOCUS       DAZUCC010000134        13690 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZUCC010000134 DAZUCC010000000
VERSION     DAZUCC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.135"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(130..1350)
                     /gene="fsr"
                     /locus_tag="ACHY09_003368"
     CDS             complement(130..1350)
                     /gene="fsr"
                     /locus_tag="ACHY09_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFS3886299.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            1568..3220
                     /gene="ushA"
                     /locus_tag="ACHY09_003369"
     CDS             1568..3220
                     /gene="ushA"
                     /locus_tag="ACHY09_003369"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS3886300.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLIMAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNNLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKAGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3257..3736)
                     /gene="ybaK"
                     /locus_tag="ACHY09_003370"
     CDS             complement(3257..3736)
                     /gene="ybaK"
                     /locus_tag="ACHY09_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFS3886301.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3940..4734)
                     /gene="ybaP"
                     /locus_tag="ACHY09_003371"
     CDS             complement(3940..4734)
                     /gene="ybaP"
                     /locus_tag="ACHY09_003371"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFS3886302.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEKRISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4867..5169
                     /locus_tag="ACHY09_003372"
     CDS             4867..5169
                     /locus_tag="ACHY09_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3886303.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            5205..5546
                     /gene="ybaQ"
                     /locus_tag="ACHY09_003373"
     CDS             5205..5546
                     /gene="ybaQ"
                     /locus_tag="ACHY09_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFS3886304.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5604..8108)
                     /gene="copA"
                     /locus_tag="ACHY09_003374"
     CDS             complement(5604..8108)
                     /gene="copA"
                     /locus_tag="ACHY09_003374"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFS3886305.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTNEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            8371..9303
                     /gene="glsA"
                     /locus_tag="ACHY09_003375"
     CDS             8371..9303
                     /gene="glsA"
                     /locus_tag="ACHY09_003375"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS3886306.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            9306..10598
                     /gene="ybaT"
                     /locus_tag="ACHY09_003376"
     CDS             9306..10598
                     /gene="ybaT"
                     /locus_tag="ACHY09_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS3886307.1"
                     /translation="MMNTEGNNDNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            10629..10726
                     /locus_tag="ACHY09_003377"
                     /pseudo
     CDS             10629..10726
                     /locus_tag="ACHY09_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10723..11130
                     /gene="cueR"
                     /locus_tag="ACHY09_003378"
     CDS             10723..11130
                     /gene="cueR"
                     /locus_tag="ACHY09_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFS3886308.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(11131..11861)
                     /locus_tag="ACHY09_003379"
                     /pseudo
     CDS             complement(11131..11861)
                     /locus_tag="ACHY09_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11933..12139)
                     /locus_tag="ACHY09_003380"
     CDS             complement(11933..12139)
                     /locus_tag="ACHY09_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886309.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(12136..12558)
                     /locus_tag="ACHY09_003381"
     CDS             complement(12136..12558)
                     /locus_tag="ACHY09_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886310.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(12644..13660)
                     /locus_tag="ACHY09_003382"
     CDS             complement(12644..13660)
                     /locus_tag="ACHY09_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3886311.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     TQTPARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLSNTQKRVADNSQQINTLNNHYSSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
BASE COUNT         3331 a         3482 c         3580 g         3297 t
ORIGIN      
        1 attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac agaaggcggt
       61 ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt gcctttggct
      121 gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca tccccaatag
      181 tggcaggaaa gcacagattt tatagactaa ctcgatgctg gtgtgatcgg cgataagccc
      241 cagaactgcc gctcccagac ctcccatgcc aaaagcaaaa ccgaaaaaga gtccagaaac
      301 cataccgata cgtcccggaa gtagctcctg agcgtagacc agaatggcag agaatgccga
      361 agcgaggata aatccaataa tcaccgttaa aacccccgtc cagtgcaggc tggcgtaggg
      421 taaaatcagc gtaaacggcg caacgccgag gatagagccc caaatcacat atttccgtcc
      481 aattttatcc cctacaggcc cgccaatcac cgtacctgcc gcaacggcaa acaggaaggc
      541 aaacaaatga agctgagcat tctggataga taatccgaat ttttgcatca gataaaaggt
      601 gtaatagctg ctgatgctcg ccatatagaa atatttcgag aaaatgagga ttaacagaat
      661 gctgaccgcc agaacaactt tattgcgcgg cagtggattg ataatcgtcg ctttgggttt
      721 tcctttattc attcggtgct gtgccgagta ccaacggctg atttgcgcca acaccacgat
      781 cgccagcagt gccgcaagca caaaccaggc aacgttgcct ttgccataag gcgcgataat
      841 caccgccgcc agcaaggggc ccagggaact gccaaagttg ccgccgacct gaaagataga
      901 ttgcgccagg ccatgacgcc cgccggaagc catacgggcc acgcgagaag attccggatg
      961 aaagaccgat gaaccggtac cgaccagcgc cgctgccagc agaactgcgc cgaaactgcc
     1021 cgccagcgcc agcagcacca gaccacttaa ggtaaagcac atgccaattg gcaacgacca
     1081 cggcatcgga tatttatcgg tccagtagcc gacaactggt tgcagtagcg aagaggcgag
     1141 ctggaaggtg agggttatca tgccaatctg cataaatgtc agagaaaact ctgactgaag
     1201 cagcggataa atcgccagaa tcagcgattg gatcatgtcg ttcagcagat gtgagaggct
     1261 gatagcacct aaaataccaa acgatgttcg ggccttggtc gttgacgcag ccgcgcccgc
     1321 cacaggctgg gtttgttcac tcattgccat aggaaagtca ctttttcagg gttgcgatgt
     1381 aaagaatgat cttatttgtg attattaccc gactaacata cctgtatgcg ccgcctgaag
     1441 gaagtctcaa cgctgaatac agaatttcta atctggatac agatttatct tcaccggacg
     1501 cagacttgtc tatgatgtcg cgtcatacta tttttcaaca cgttgaaatc aggtcaggga
     1561 gagaagtatg aaattattgc agcggggcgt ggcgttagca ctgttaacca catttacact
     1621 ggcgagtgaa gctgctctgg cgtatgagca ggataaaacc tacaaaatta cagttctgca
     1681 taccaatgat catcatgggc atttttggcg caatgaatat ggcgaatatg gtctggcggc
     1741 gcaaaaaacg ctggtggatg gtatccgcaa agaggttgcg gctgaaggcg gtagcgtgct
     1801 gctactttcc ggtggcgaca ttaacactgg cgtgcccgag tccgacttac aggatgccga
     1861 acctgatttt cgcggtatga atctggtggg ctatgacgcg atggcgatcg gtaatcatga
     1921 atttgataac ccgctcaccg tattacgcca gcaggaaaag tgggctaagt tcccgttgct
     1981 ttccgccaat atctaccaga aaagtaccgg cgagcgcctg tttaaaccat gggcgctgtt
     2041 taagcgtcag gatctgaaaa ttgccgttat tggcctgacg acggatgaca cagccaaaat
     2101 tggtaatccg gaatatttca ctgatatcga atttcgtaag cccgctgatg aagcgaagct
     2161 ggtgattcag gagctgcaac agacagaaaa gccagacatt attatcgcgg cgacccatat
     2221 ggggcattac gacaatggtg agcacggctc taacgcaccg ggcgatgtgg agatggcacg
     2281 cgcgctgcct gccggatcgc tggcgatgat cgtcggtggc cactcgcaag ctccggtctg
     2341 catggcggca gaaaacaaaa aacaggtcga ttacgtgccg ggtacgccat gcaaaccgga
     2401 tcaacaaaac ggcatctgga ttgtgcaggc gcatgagtgg ggcaaatacg tgggacgggc
     2461 tgattttgag tttcgtaatg gcgaaatgaa aatggttaac taccagctga ttccggtgaa
     2521 cctgaagaag aaagtgacct gggaagacgg gaaaagcgag cgcgtgctgt acacccctga
     2581 aatcgctgaa aaccagcaaa tgatctcgct gttatcaccg ttccagaaca aaggcaaagc
     2641 gcagctggaa gtgaaaatag gcgaaaccaa tggtcgtctg gaaggcgatc gtgacaaagt
     2701 acgttttgta cagaccaata tggggcggtt gattatggca gcacaaatgg atcgcactgg
     2761 tgccgacttt gcggtgatga gcggaggcgg aattcgtgat tctatcgaag caggtgatat
     2821 cagctataaa aacgtactga aagtgcagcc attcggcaat gtggtggtgt atgccgacat
     2881 gaccggtaaa gaggtgatcg attatttgac cgccgtcgcg cagatgaagc cagattcagg
     2941 tgcctacccg caatttgcca acgttagctt tgtggcgaaa gacggtaaac tgaacaacct
     3001 gaaaatcaaa ggcgaaccgg tcgatccggc gaaaacttac cgcatggcga cattaaactt
     3061 caatgccacc ggcggtgatg gctatccgcg ccttgataac aaagcgggct atgtgaatac
     3121 cggctttatt gatgccgaag tgctgaaagc gtatatccag aaaagctcgc cgctggatgt
     3181 gagtgtttat gaaccgaaag gtgaggtgag ctggcagtaa tccgaaagtg ccggatgttc
     3241 gcatccggca caatgcttaa tcgcgacggg cgatatcagc aaatttggca tcgaggatct
     3301 ttgccagatc gcctgccgcc agttcgatat ccagcccgcg cttgccgccg gaaacataaa
     3361 tagtggcaaa ttcttgtgcg ggggcgtcga taatcgttgg cagacgtttt ttctgcccca
     3421 gtgggctaat cccccctacc agataccccg tcgaacgctg cgcgaccatc ggatcggcca
     3481 tctcaacttt cttggcaccc agcgcttttg ctactttttt aagatccagt tgacctgcga
     3541 ccggcgtaac ggccacggca aggtgtttca tatcaccgtt cactgcgacc agcagcgttt
     3601 tgtagacctg atccggattc aaacctaatt ttttgacgac ttcatcgcca aaattggttt
     3661 cagccggatc gtgctcgtag gtgtggattt gaaacgaaat cttgtttttt tcgagtaatt
     3721 taactgcggg tgtcatggta attttcctgc cttaaacaaa aaaacgaagc aagcatacgc
     3781 ctgatggcgg tccaaaaaaa agagtcatct tgcctaagag tattggcagg atggtgagat
     3841 tgagcgacaa tcgagttaca ccgtcgctta aagtgacggc ataataataa aaaaatgaaa
     3901 ttcctctttg acgggccaat agcgatattg gccatttttt tagcgcaaca tctgcggcaa
     3961 attcccttct ccatacaggt gtagtgcacc aaccgcgacc acatagcgcc ccggcggcat
     4021 ggcgcataat ttgtcccgcc aggcgagatt tcgctgatgc atcagcacat cgtacagcga
     4081 ctgactgaac gtattaggca gcgttatttc attattttgc ggcggcgcat tcagccacca
     4141 gctcatcatt tgttgcagca accgtgcgtt ggtgtgccag tgggtcagcg tatcgtccag
     4201 cagcgccagt ccgttgtcag ggagctggag caacatggca atctggtttt cagccccttc
     4261 cagttcaatc acgggtttat gttgttgctt agccgcctgc aatagctggt aatcgatacc
     4321 gtattctgcc cgcagcccca gtttttgcgc ctgcgtcgcc tgaagaacca tcgcgatttg
     4381 ccacagcggt tgagtagaaa agagcgacgg agaaatgccc atctcctggc taatatgctg
     4441 caggttttgg agttgctcct cgctaatgcg cttttccagg gcctcgcagg caggcaaatt
     4501 ggcaaaaggc gtatcgctgg tggaaacatc cgcctcgacg atcagcgcat cggcgttttt
     4561 gagctttttg agcaaacggg tgggcagggg agccatatcg tggctaccca tatgaatact
     4621 gccgatcaga tgaaaatggc gattgccggg aagagagata tcgatagcag gccaggtgta
     4681 atgattaccg cgcagcgcgg cccaaagcgt ttttacccgg tacaacagat ccatacgacc
     4741 tccctttgtg aaatatcatg ctagcgcgcg gggagggatg gcgcaaatgg agttgattgt
     4801 tgcacttgcg cacagtgctt gtgtgcatta caatgattaa ataatggaca aggagatgag
     4861 agaggaatgg ctcaaaagaa aaatataagg agttttcgtg atgcctggtt ggctgatttt
     4921 tttgtccatt caacgccaca tcgaaagatc cctgctgaaa tccataccac gttatcacga
     4981 aagttggaca tcattaatgc tgccacttct catcgggatt tacgctcccc tccggggaac
     5041 cgatacgaag agttatccgg gaaattacag gaatactcct ccattagagt gaataaacag
     5101 taccgactga tttttaaatg ggttaatggc aaggccgaag aattgttttt agacccacac
     5161 aactactaaa acatagctgt cgtattgacg agaaaggtaa aactatgaaa caggcaacaa
     5221 gaaaaccgac gacacccgga gatattcttc tctatgaata tctggaaccg ctcgatttga
     5281 aaatcaatga gttagcagag ttgctgcatg ttcatcgtaa tagcgtcagt gcactgatca
     5341 ataacaatcg taaactcacc actgagatgg cattccgtct ggcgaaagtt tttgatacca
     5401 cagtcgattt ttggctaaac ctccaggcgg cagttgatct ttgggaagtt gaaaacaaca
     5461 tgcgtaccca ggaagaattg ggacggattg aaacagtggc tgaatatttg gcacgccgtg
     5521 aagagcgtgc aaaaaaggtc gcgtagaata atttccctat aacggatgcg ccaccctaaa
     5581 gcagtgcatc cgcaatgatg tacttattcc ttcggtttaa accgcagcaa ccggttggcg
     5641 ttactcacta cggtaatcga cgagagcgcc attgccgctc cggcgaccac cgggttaagc
     5701 agcgtaccgg tgaacggcca caaaataccg gcggcgaccg gaataccaat gctgttgtag
     5761 ataaacgcac cgagcaggtt ctgcttcata ttgcgcagcg ttgcgcggga aatcgcgagc
     5821 gcatccgcaa cacccatcag gctatggcgc atcagggtaa tcgccgcggt ttcaatggca
     5881 acatcactgc cgccacccat tgcaatgccg acatccgcct gagccagcgc tggcgcgtcg
     5941 ttaatgccat cgcccaccat tgccacctga cgtccttcac tttgcagacg tttgatcgct
     6001 tcggctttac cgtccggcag caccccggct atcacctcat caatccctgc ttctttggcg
     6061 atcgcattgg cggtggttgg gttatccccg gttagcatca ccagacgata tcccgcttta
     6121 tgcaggcgtt gcagcgccgc cacgctatca ctacgcaatg gatcgcgtac agccagcagg
     6181 gctaccgctt tcccgtcaac cgccagcagc acaggcgttg ccccttgcga tgcctgagct
     6241 gaaatatctg cttcaatcgc tttagtatca acttgctggt catttaacag cgcctgattg
     6301 cccagcaata acgcatgacc ttcagcttca ccgctcacgc ccagcccgcg caatgtgcgg
     6361 aaaccgttga cctgcggcag ctgcatatcg cttgctttat cgaggatcgc ccgtgccagc
     6421 ggatggctgg aaccttgttc cagtgccgcc gccagacgca gcgcctgcgc ttcgtcaaaa
     6481 tcagcaaatg tttttactgc gacaacctgc ggcttccctt cggtcagcgt cccggtttta
     6541 tcgaacacca cggtgtcgag cgtactggcg cgttgcagcg cgtcggcgtc ccgcaccagc
     6601 acgccaaact cagccgcccg cccgacgccc gaaataatcg acatcggcgt cgccagtccc
     6661 agcgcacacg gacaggcgat aatcagcacc gtggtggcaa tcaccagggt ataaacaatt
     6721 tgcggtgccg ggccaaagaa ataccagatt gccgcactga ccagcgcaat gactaccacc
     6781 accggcacaa acacagcgga gattttatcc gccagttgac cgatttctgg cttgctgctc
     6841 tgggcctgtc gcaccatgcg aatgatccgt gacagcgtag tatggctgcc gaccgcactg
     6901 gcgcgaaaca gcacactgcc gtcctgtacc actgtcccgg catggacgct atcgccttcg
     6961 cctttttgct gcgggattgg ttcgcccgtc agcatcgctt catccagcca cgcttcgccc
     7021 tgggtaattt caccatctac cggcacccga tcacccgtcg tcaggcgcag caacatacct
     7081 ggctgaacat ctgccagcgg cacgtttttt tcaccttcgt tggtgaccag gcgcgccgtc
     7141 ggtggggtta aatcgagtaa tttctccagc gccttagaag aacgctgacg tgcgcgcgct
     7201 tccagcatat gaccgagatt aatcagaccg ataatcatcg cactggcttc atagtaaaga
     7261 tgtcgcgctt ccatcgggaa ccactgcggc cacaggttga cgctcattga atagagccac
     7321 gccacgcccg tacccagcgc caccagcgta tccatcgtcg ctgcaccgtt cagcaggctt
     7381 ttccatgcgc tgcggtaaaa atggccgccg gcgaaaacca ttaccgccag ggttatcagg
     7441 ccgataacca accacaggct gcggttgtcg gcggtgacca tcatgttatc gccgatcatc
     7501 ccccagacca tcaccgggat acccaccgcc agtgcgacaa tcgcctgcca gcggaagcgc
     7561 ttcatcgtag cgacggcggt ttcttgctgg cgctcgcggc gtttagcgtc atcttcaatc
     7621 gcttccgcgc cgtagcccgc tttttccacc gcctgcacta aatcttgtgg ggaggcactg
     7681 cccatcacca gcgcagtacg ctccgccagg tttacccgtg cctgagtgac gcccggtacg
     7741 ctttgcaacg cattttgtac gcgggtgaca cagctggcgc agctcatgcc gctcagcagc
     7801 aactgctggc tgtcatcgtc atcggcggtc gctgccggaa gcgcctcaga aaccgctgtc
     7861 agtgcttccg acgggattga tgactccgcc agcggtttag cctttgggtg gcttacagat
     7921 gcgtcataac ccgcttgttt gatggtttcg atcagctgtt ctgcactggc agtcccggta
     7981 acgtgcgctt cagtgacaga cacatccgcc tgctcaacat ccgggcgctg ttcgagactt
     8041 tctttcacgc gtttaacgca gtgaccgcag gacaggccgt ccagggtcag gtcgatagtt
     8101 tgtgacataa aacactcctt ttaagacagt tttgactggc tgtgataaag gttaaacctt
     8161 ccagcaaggg gaaggtcaag aaattaataa accaggcggg taaaagtacg taaagattaa
     8221 aaaatcggct cgatttgcat caggattaga catttatctc tttgttttcc tgtagttaag
     8281 ttgcgggtgc taagttaaat caggatgcct gaaaattggc accggggtga ggaattacct
     8341 cccgcatcta taaaaaggag ttaacaaaag atgttagatg caaacaaatt acagcaggca
     8401 gtggatcagg cttacaccca atttcactca cttaacggcg gacaaaatgc cgactacatt
     8461 ccctttctgg cgaatgtacc tggtcaactg gcagcagtgg ctattgtgac ctgcgatggc
     8521 aacatctatc gcgcgggtga tagtgattac cgctttgcgc tggaatccat ctcgaaagtc
     8581 tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg cggtacagga caaaattggc
     8641 gctgacccga ccggattgcc ctttaactca gttatcgcct tagagttgca tggcggcaaa
     8701 ccgctgtcgc cactggtaaa tgctggcgct attgccacca ccagcctgat taacgctgaa
     8761 aatactgaac aacgctggca gcgaatttta catatccaac aacaactggc tggtgagcag
     8821 gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa ccaacttcca taaccgggcc
     8881 attgcctggc tgctgtactc cgccggatat ctctattgtg atgcaatgga agcctgtgac
     8941 gtgtacaccc gtcagtgctc cacgctcatc aatactgttg aactggcaac gcttggcgcg
     9001 acgctggcgg cgggtggtgt gaatccgctg acgcataaac gcgttcttca ggccgacaac
     9061 gtgccgtaca ttctggctga aatgatgatg gaagggctgt atggtcgctc cggtgactgg
     9121 gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg gcggtattct ggcggtcgtc
     9181 cctggcgtga tgggaattgc cgcgttctca ccaccgctgg acgaagaagg caacagtgtt
     9241 cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg gctataacgt gtttaagggc
     9301 tgatcatgat gaacacggaa ggtaataacg ataacaaacc cctcggtcta tggaacgtcg
     9361 tttccatcgg tattggggca atggtggggg cggggatctt cgcgctgctg gggcaggctg
     9421 cgttgctaat ggaagcctcg acctgggtcg cctttgcttt tggcggtatt gtggcgatgt
     9481 tttccggtta tgcctatgcg cgcctggggg cgagctatcc cagcaatggc ggtattatcg
     9541 acttctttcg tcgcggatta ggcaacggcg tcttttcgct ggcactctcg ttattgtacc
     9601 tgttgacgct ggcggtgagc atcgccatgg tcgcccgtgc ttttggcgct tatgccgtgc
     9661 agtttttgca tgaaggcagt caggaggagc accttatatt gctctacgcg ttggggatca
     9721 ttgcggtgat gacgcttttc aactccttaa gcaaccatgc ggtagggcgg ctggaagtga
     9781 tcctcgtcgg cattaaaatg atgatcctgt tattgctgat cattgccggt gtctggtcgc
     9841 tgcaaccggc gcatatttcc gtctctgcgc cccccagctc cggtgcgttc ttctcctgta
     9901 tcgggataac cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg gcggataaag
     9961 tgaaagatcc gcaggtcatt atgccacggg cgtttctggt ggcgattggc gttaccacgt
    10021 tgctttatat ctcgctggcg ctggttttgc ttagcgatgt atcggcatta gagttagaaa
    10081 aatatgccga taccgccgta gcgcaggctg cttctccgct gctcgggcat gtgggttatg
    10141 tgatcgtcgt catcggcgct ttactggcga cggcttcagc cataaacgcg aacctgttcg
    10201 ccgtgtttaa catcatggac aacatgggca gcgaacgcga actgccgaag ctaatgaata
    10261 aatccctgtg gcggcagagt acctggggca acatcattgt cgtggtgctg attatgctga
    10321 tgacggcggc gctgaattta ggctcactcg ccagcgttgc cagcgccacc tttttgatct
    10381 gctatctggc ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac gcctcgttgc
    10441 cgattcttat cgttggtacg ttggttatgt tgttggtgat cgttggcttt atctacagtc
    10501 tctggtccca gggcagccgt gcgctgatat ggattattgg cgcactctta ctcagcctta
    10561 ttgtggcaat ggtcatgaag cgcaataaaa ctgtataaca tctctctgtg cgcagtacct
    10621 cctgtattat tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc ttgaatatct
    10681 gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat tagcgatgta
    10741 gcaaaaatta ccggcctgac cagcaaagcc attcgcttct atgaagagaa ggggctggtg
    10801 acgccgccga tgcgcagcga aaacggttat cgcacctaca cgcagcagca tctcaacgaa
    10861 ctgaccttac tgcgccaggc acgccaggtg ggctttaacc tggaggagag cggcgaactg
    10921 gtgaatctgt ttaacgatcc gcagcgtcac agcgccgacg tcaaacgtcg tacgctggag
    10981 aaagtggcgg agatcgaaca gcatatcgaa gaactgcaat ccatgcgtaa ccagctgctg
    11041 gcgctggcga atgcctgccc cggtgatgac agcgccgact gcccgattat cgaaaatctc
    11101 tccggctgct gtcatcatcg ggcggggtga ttaaggcttg cgaaaatagt cgtccattat
    11161 tttcgtcagc tccggacgtt ccccgtcatc attgatggca aaatccagaa aaaaagcatc
    11221 tgcttgcggc gttcggtttt gcgccatcaa ccggaaggtg agggggcctg accaggtttc
    11281 ggatgggctg atgtgcttat cagggccgaa actctcccat gcgttgggcg gtaatgtttt
    11341 gcacgcggtg agttttggta tgtggtattg cagctctgtt ttgtgcagac attccccgcc
    11401 aggaaatacc agggtttgtt gctcatcatt aaaatgggtt gccgtgctga gcaccagtgt
    11461 tttgagcggt cgcccttcca gcgcggcggc agtagcctta tggtgtccgt cgagcagaac
    11521 gcagagatag gaatcaggga gataccaggc cagagcgcgg gcgattccag ttttcgagag
    11581 cagacaaaaa ccgggcggct ccctgttcac cctccgcaaa accgctgtag acaaattccg
    11641 cacaggtagg gcaaccagcc tcggaccaga aacgtattaa cgggacatcc ttgccagcag
    11701 agcgaatgga taagaccgtg cgagtgcgga tcgtaaatca cacattgacg cagaaatcct
    11761 ttcccggact tcaccgaaaa aatgacgccc gcagcggtat ttttcagcag acgtacttca
    11821 ggttttcgaa aggggaaaaa gcatggccac tccttagcca aaatttcagt tccctgaact
    11881 gtgccagaaa attacgctgg tgactattgc tctggaaatg gaaacagtgt ccttacccat
    11941 taatccccgt ttgtaccgaa agaatatgtt gcaaactttc taccataatg cgccccgact
    12001 gaaataagcc ttcctggatt tcattgttaa cattcacagc gtgaaccagg gaggctaata
    12061 acaccacttg caagaaggca aaaataaacg caccgataat cataatatgc gtcaggggga
    12121 ttttcctttg ctccttcatt ttaaataacc cacaaactgg ccttctttca ttaaggcgac
    12181 ttttcctgtg tgatgataaa caatgacatt atacagaccc gactgataaa ccgtggcatc
    12241 ggcattatct tcatctatgg cacataatga atcattgaga taagcaaaat tcttactact
    12301 cattctaaag gtcatgccaa atcctttata aatggctttt tttgccgcct gggcatgttg
    12361 tgcggcttct gcatttttaa atgcctgcgc ttcagaagca cttaaaatag ccgtctggcg
    12421 ctgctgttct gcgagataac aggtgtcatg gctgacgccc tgcgcctgac aatctgcgat
    12481 gcgggaagcg ggtgagctac agccagccag taatataacc gccagcaggg aaaaggattt
    12541 aatggagcta ttcatcattg ttaaccttaa ataatgtgcg aattaatgaa gaggtgaaac
    12601 ccggcaagct tccttgctct tatttatatt gaggattgaa atattagaac gaatatccta
    12661 cgccgacacc tgcgccgaaa tcagattgcg tatccgcaga aataccggct ttaagtaccg
    12721 tatgcgttcc ggcattaaaa gatgttccga cagacaccgc agattcaccg ttgaaggttc
    12781 ccgctcccgc tgacaccatc atcacgtcac cggttttaac ctgtggttgt gaggcgatag
    12841 cgatggcaga tgcagttccc gcattggctt ctttacggtt gtcctcaact tcgtttttca
    12901 gcgaactgta atggttattg agcgtgttaa tttgttggct gttatctgcg acccgttttt
    12961 gcgtgttgct taactgttgc tgcgtttgtg cggaatcttg tctcagactt tcaatttgct
    13021 catgtgcctg gacagacttc tgcgccagac tttgcatttg tttatcgtat tgagcccttt
    13081 ctttttgctg ttgtgctgcc acatcggttg tcagtgcatt cattttctgc ccttgttccg
    13141 tggtagtgcg ctgattagcc tcattttgag ccgccagtct ggcattaatc atctcctgat
    13201 aatgcgcagt attttgcgta tctccagtcg cggcgagcgt tttttgtgtg gctacgatct
    13261 gcgcctgttg ctctgtaatt tgcgtttgct gggcatcagt taccgcagac tgtcgggcgt
    13321 tgatcatgct ctgataacgg gcaggtgtct gtgtatcacc tgtagcggca acggtttttt
    13381 gtcctgcgtc aggatctgtc tggacagtgg gagtctgagt ggtatcaggt gtttcagaag
    13441 ggatgtagtg atgttcagtc aaataggtat acgcagcggg gctgttgtct gtgcgttcta
    13501 aaaagcgggt aatcttctgc tcaatctccg attgcgacat gccagagtta atggctgaaa
    13561 gctctgttgc cgccgcttca atggtatcac cagctaaaac aacagggctg gatgttgctg
    13621 aaattatgag tgccagtaaa gctacgttta cagttttcat tcattttctc ttgttcaaaa
    13681 taagtcgcat
//
LOCUS       DAZUCC010000135        13621 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZUCC010000135 DAZUCC010000000
VERSION     DAZUCC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.136"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            147..1256
                     /gene="livK"
                     /locus_tag="ACHY09_003383"
     CDS             147..1256
                     /gene="livK"
                     /locus_tag="ACHY09_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFS3886312.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1304..2230
                     /gene="livH"
                     /locus_tag="ACHY09_003384"
     CDS             1304..2230
                     /gene="livH"
                     /locus_tag="ACHY09_003384"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFS3886313.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2227..3504
                     /gene="livM"
                     /locus_tag="ACHY09_003385"
     CDS             2227..3504
                     /gene="livM"
                     /locus_tag="ACHY09_003385"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFS3886314.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGSFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3501..4268
                     /gene="livG"
                     /locus_tag="ACHY09_003386"
     CDS             3501..4268
                     /gene="livG"
                     /locus_tag="ACHY09_003386"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFS3886315.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4270..4983
                     /gene="livF"
                     /locus_tag="ACHY09_003387"
     CDS             4270..4983
                     /gene="livF"
                     /locus_tag="ACHY09_003387"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFS3886316.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5071..5292
                     /locus_tag="ACHY09_003388"
     CDS             5071..5292
                     /locus_tag="ACHY09_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS3886317.1"
                     /translation="MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEF
                     ERYQKAKLDAEFDAIMAIHGSEIKELADK"
     gene            5289..5666
                     /locus_tag="ACHY09_003389"
     CDS             5289..5666
                     /locus_tag="ACHY09_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS3886318.1"
                     /translation="MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNK
                     YEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALFITLLFLKRNGIILAANPEFVEL
                     TVDVAAGKVSLEDIVTRLRQRGT"
     gene            5875..7191
                     /gene="ugpB"
                     /locus_tag="ACHY09_003390"
     CDS             5875..7191
                     /gene="ugpB"
                     /locus_tag="ACHY09_003390"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFS3886319.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            7367..8254
                     /gene="ugpA"
                     /locus_tag="ACHY09_003391"
     CDS             7367..8254
                     /gene="ugpA"
                     /locus_tag="ACHY09_003391"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFS3886320.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            8251..9096
                     /gene="ugpE"
                     /locus_tag="ACHY09_003392"
     CDS             8251..9096
                     /gene="ugpE"
                     /locus_tag="ACHY09_003392"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFS3886321.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9098..10168
                     /gene="ugpC"
                     /locus_tag="ACHY09_003393"
     CDS             9098..10168
                     /gene="ugpC"
                     /locus_tag="ACHY09_003393"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFS3886322.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLKGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVLRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10165..10908
                     /gene="ugpQ"
                     /locus_tag="ACHY09_003394"
     CDS             10165..10908
                     /gene="ugpQ"
                     /locus_tag="ACHY09_003394"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS3886323.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10895..11335)
                     /locus_tag="ACHY09_003395"
     CDS             complement(10895..11335)
                     /locus_tag="ACHY09_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFS3886324.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            11455..13200
                     /gene="ggt"
                     /locus_tag="ACHY09_003396"
     CDS             11455..13200
                     /gene="ggt"
                     /locus_tag="ACHY09_003396"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFS3886325.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            complement(13238..13522)
                     /locus_tag="ACHY09_003397"
     CDS             complement(13238..13522)
                     /locus_tag="ACHY09_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFS3886326.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFIQGWYFCFESQEFL
                     ESGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         3047 a         3417 c         3898 g         3259 t
ORIGIN      
        1 agggatttta atcgctatca tcacaaaata ctgtgctaaa cccttaatca gacaggcaaa
       61 aacagtgcag tataaaaaaa gaacagtctg atttgttaac acataagaac aaagcaacac
      121 aacatcacga atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga
      181 tgattgcact ggcaatttca cacaccgcta tggctgacga tattaaagtc gccgttgtcg
      241 gcgcgatgtc cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg
      301 cgattaaaga cattaatgcc aaagggggaa ttaaaggcga taagctggtt ggcgtggaat
      361 atgacgacgc ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaacgacg
      421 gcattaaata cgttattggg cacctgtgtt cctcctctac ccaacctgca tcagatatct
      481 acgaagacga aggtattctg atgatttcgc cgggagcgac caatccggag ctgacccaac
      541 gcggttatca atacatcatg cgtactgccg gtctggactc ttcccagggg ccaacggcgg
      601 caaaatacat tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac
      661 agtatggcga agggctggcg cgttcggtgc aggacggatt gaaagcagcc aatgccaacg
      721 tcgtcttctt cgacggcatt accgctggtg agaaagattt ctccgcgctg atcgcccgcc
      781 tgaaaaaaga aaacatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga
      841 tgctgcgcca ggcccgttcc gttggcctga aaactcagtt tatggggccg gaaggggtgg
      901 gtaatgcgtc gctgtcgaac attgctggtg atgccgccga aggtatgttg gtcactatgc
      961 caaaacgcta tgaccaggac ccggcaaaca aaggcatcgt tgatgcgctg aaagcagaca
     1021 agaaagatcc gtccggacct tatgtctgga tcacctacgc ggcggtgcaa tctctggcga
     1081 ctgcccttga gcgtaccggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta
     1141 acggtgcaaa caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat
     1201 ttgattttgg tgtcttccag tggcacgccg acggttcatc gacggcagcc aagtgatcat
     1261 cccaccgccc gtgaaatgcg ggcggtttta gaaaggttac cttatgtctg agcagttttt
     1321 gtatttcttg cagcagatgt ttaacggcgt cacgctgggc agtacctacg cgctgatagc
     1381 catcggctac accatggttt acggcattat cggcatgatc aacttcgccc acggcgaggt
     1441 ttatatgatt ggtagctatg tctcctttat gatcatcgcc gcactgatga tgatgggcat
     1501 tgataccggc tggctgctgg tggcggcggg attcgtcggc gcaatcgtca ttgccagcgc
     1561 ctacggctgg agtatcgaac gggtagccta tcgtccggtg cgtaactcta agcgcctgat
     1621 tgcgcttatt tccgcaatcg gcatgtctat cttcctgcaa aactacgtca gcctgaccga
     1681 aggttcgcgc gacgtggcgc tgcccagcct gtttaacggt cagtgggtgg tgggccatag
     1741 cgaaaacttc tctgcctcta ttaccaccat gcaggcggtg atatggattg ttactttcct
     1801 cgccatgctg gcgctgacga ttttcattcg ctattcccgt atggggcgcg cctgccgtgc
     1861 ctgcgcggaa gatctgaaaa tggcgagcct gcttggcatc aacaccgacc gggtgattgc
     1921 gctgaccttt gtgattggcg cggcgatggc ggcagtggcg ggcgtgctgc tcggtcagtt
     1981 ctacggcgta attaaccctt acatcggctt tatggccggg atgaaagcct ttaccgctgc
     2041 ggtgctcggt gggattggca gcattccggg ggcgatgatt ggcgggctga tcctggggat
     2101 tgcggaggcg ctctcttctg cctatctgag tacggaatat aaagatgtgg tctcattcgc
     2161 cctgctgatc ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga
     2221 gaaagtatga aaccgatgca tattgcaatg gcgctgctct ctgccgctat gttctttgtg
     2281 ctggcgggcg tctttatggg cgtgcaactg gagctggatg gcaccaaact ggtggtcgac
     2341 acggcttcag agatccgttg gcagtgggtg tttatcggca cggcggtggt ctttttcttc
     2401 cagcttgtgc ggcctgcttt ccagaaaggg ctaaaaagcg tttccgggcc gaagtttatt
     2461 ctgcccgcga ttgatggctc cacggtgaag cagaaactgt tcctcgtggc gctgctggtg
     2521 cttgcagtgg cgtggccgtt tatggtgtcg cgtgggacgg tggatatcgc cactctgacc
     2581 atgatctaca ttattctcgg tctcgggctg aacgtggttg ttggtctttc tggtctgctg
     2641 gtgctgggat acggcagctt ttacgccatc ggcgcttaca cttttgcgct gctcaatcac
     2701 tattacggtc tgggcttctg gacctgtctg ccgattgccg ggttgatggc ggcggcggcg
     2761 ggcttcctgc tcggttttcc ggtgctgcgt ctgcgcggtg actatctggc gatcgttacc
     2821 ctcgggttcg gcgaaattgt gcgcatattg ctgctcaata acaccgaaat taccggcgga
     2881 ccgaacggta tcagtcagat tccgaaaccg acgctcttcg gactcgagtt cagccgtacc
     2941 gcccgcgaag gcggctggga cacgttcagt aatttctttg gcctgaaata cgatccctcc
     3001 gatcgcgtca tcttcctcta cctggtggcg ttgctgctgg tggtgttaag cctgtttgtc
     3061 attaaccgcc tgctgcggat gccgctgggc cgtgcgtggg aagcgttgcg tgaagacgag
     3121 atcgcctgcc gttcgctggg cttaagcccg cgtcgtatca agctgactgc ctttaccatc
     3181 agcgccgcgt ttgctggttt tgccggaacg ctgtttgctg cgcgtcaggg ctttgtcagc
     3241 ccggaatcct tcacttttgc cgaatcggcg tttgtgctgg cgatagtggt gctcggtggt
     3301 atgggttcgc aatttgcggt gattctggcg gcaattttgc tggtggtgtc gcgagagttg
     3361 atgcgtgatt tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg
     3421 atctggcgtc cgcagggctt gctgcccatg acgcgcccgc aactcaagct gaaaaacggc
     3481 gcagcgaaag gagagcaggc atgagtcagc cattattatc tgttaacggc ctgatgatgc
     3541 gcttcggcgg cctgctggcg gtgaataacg tcaatcttga actgtacccg caggagatcg
     3601 tctcgttaat cggccctaac ggtgccggaa aaaccacggt ttttaactgc ctgaccggtt
     3661 tctacaaacc caccggtggc actattttac tgcgcgatca gcatctggaa ggtttgcccg
     3721 gccagcaaat cgcccgcatg ggcgtggtgc gcaccttcca gcatgtgcgt ctgtttcgtg
     3781 aaatgacggt gattgaaaac ttgctggtgg cgcagcatca gcaactgaaa accgggctgt
     3841 tctccggcct gttgaaaacg ccttctttcc gccgcgccca gagcgaagcg ctcgaccgtg
     3901 ccgcaacctg gcttgagcgc attggtttgc tcgaacacgc taaccgtcag gcgagtaacc
     3961 tggcctacgg tgaccagcgc cgtctggaga tcgcccgctg catggtaacg cagccggaga
     4021 ttttaatgct cgacgaaccg gcagcaggtc ttaacccgaa agagacgaaa gagctggatg
     4081 agctgattgc cgaactgcgt aaccatcaca acaccactat cttgttgatt gaacacgata
     4141 tgaagctggt gatggggatt tcagacagaa tttacgtggt caatcagggg acgccgctgg
     4201 caaacggtac gccggagcag atccgtaata acccggacgt gatccgtgcc tatttaggtg
     4261 aggcataaga tggaaaaagt catgttgtcc tttgacaaag ttagcgccca ctacggcaaa
     4321 atccaggcgc tgcatgaggt gagcctgcat atcaatcagg gcgaaattgt cacgctgatt
     4381 ggcgcgaacg gggcggggaa aaccaccttg ctcggcacgt tatgcggcga cccacgcgcc
     4441 accagcgggc gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc
     4501 atgcgcgaag cggtggcgat tgtcccggaa ggacgtcgcg tcttctcacg catgacggtg
     4561 gaagagaatc tggcgatggg cggctttttt gctgaacgcg accagttcca ggagcgcata
     4621 aagtgggtgt atgaactgtt tccacgtctg catgaacgcc gtgttcagcg ggcaggcacc
     4681 atgtccggcg gtgaacagca gatgctggcg attggtcgcg ctttgatgag taacccacgt
     4741 ttgttattgc ttgatgaacc ctcgctcgga ctggcaccga ttatcatcca gcaaattttc
     4801 gacaccatcg agcaactgcg cgagcagggg atgaccatct tcctcgtcga gcagaacgcc
     4861 aaccaggcgc tgaagctcgc tgatcgcggc tacgtgctgg aaaacggtca tgtggtgctc
     4921 tccgatactg gcgatgcgct gctggcgaac gaagcggtga gaagtgcgta tttaggcggg
     4981 taataatatt ttgagtaaaa aggagtcgaa tgactccttt tttgtacttg ttagttgtac
     5041 aatttaattg tacttaatat ggaggatatc atgcatacca tgaactacag cgaagcgcgg
     5101 cagaaccttg cattagttct ggaaacagcg gcgacaggta ctccggtaac gatcacgagg
     5161 cgtgggcata aggcagctgt gattatcagc gcagaagagt ttgaacgtta ccagaaggct
     5221 aaactggacg cagaatttga cgccatcatg gctattcatg gcagcgaaat taaggagtta
     5281 gcggataaat gaccattcag tttatctcag cggaggagat aatttatttc cacgataaat
     5341 tgcttagcgt tacaccaggt gtaacaggaa tgtccgatcc agggcgagca gaagcgcttt
     5401 tgtaccgggt actgaataag tatgagtatg aaggcgtttc tgatctttgg ctgctggcgg
     5461 cgatgcatct attggcaatc tcgcgcggtc atatctttaa tgatggtaat aagcgcaccg
     5521 cactgtttat cacgctatta ttccttaaac gtaacggcat catacttgct gcaaacccag
     5581 agtttgtaga attgacggtt gatgtggcgg cgggaaaagt atcgttagaa gatatcgtta
     5641 cccgcttgcg tcaacgcggt acctgacaag gttcttagcc attctgcttt ttatccgtgc
     5701 cccatctccc attttccctc acccacgccg tcaccgcctt gtcatctttc tgacacctta
     5761 ctatcttaca aatgtaacaa aaaagttatt tttctgtaat tcgagcatgt catgttaccc
     5821 cgcgagcata aaaagcgtga attcgcgcat ccggtacaac aagagagata aacgatgaaa
     5881 ccgttacatt atacagcttc agcactggcg ctcggactgg cgttaatggg gaatgcacag
     5941 gcagtgacga ccattccgtt ctggcattct atggaagggg aactgggtaa agaggtggat
     6001 tctctggccc aacgttttaa cgccgaaaac ccggattaca aaattgtacc gacctataaa
     6061 ggcaactacg aacagaattt aagcgcgggg attgccgcat ttcgtaccgg caacgcgccg
     6121 gctattttgc aggtttatga agttggcacc gccaccatga tggcgtcgaa agccattaaa
     6181 ccggtatatg acgtgtttaa agaggcgggg attcagttcg atgagtcgca gtttgtgccg
     6241 acggtttcag gctactactc cgacagcaaa accggccact tactctccca gccgttcaat
     6301 agctcgactc ctgttctcta ttacaacaaa gacgccttca agaaagccgg tttagacccg
     6361 gaacagccgc cgaaaacctg gcaggatctg gcggactatg ccgcgaaact gaaagcctcc
     6421 ggtatgaagt gcggctacgc cagcggctgg cagggctgga tccaactgga aaactttagc
     6481 gcctggaacg gtctgccgtt tgccagcaaa aacaacggct ttgacggcac agacgcggtg
     6541 ctggagttca acaagccgga gcaggtgaaa cacatcgcta tgctcgaaga gatgaacaag
     6601 aagggcgatt tcagctacgt cgggcgtaag gatgaatcca ctgagaagtt ctataacggt
     6661 gattgcgcga tgaccaccgc ctcttccggt tcgcttgcca acattcgtga gtacgccaaa
     6721 tttaactacg gcgtaggcat gatgccttac gacgccgatg cgaaagatgc accgcaaaac
     6781 gccattatcg gcggggccag cctgtgggtg atgcagggca aagataaaga aacgtatacc
     6841 ggcgtggcga agttcctcga cttcctcgcg aagccagaaa acgctgccga gtggcatcag
     6901 aaaaccggtt atctgccaat caccaaagcg gcgtatgacc tgacccgtga gcagggcttt
     6961 tatgagaaaa acccaggggc ggataccgcg acgcgtcaga tgctgaataa gccgccgtta
     7021 ccgttcacca aagggctgcg tctgggcaac atgccgcaga tccgcgtgat tgtggatgaa
     7081 gagctggaga gcgtgtggac cggtaagaag acaccgcagc aggcgctgga taccgccgtt
     7141 gagcgtggga accagttact gcgccgcttt gagcagtcta cgaagtctta attctcttac
     7201 cctcacccta accctctccc aaagggagag gggaccgatc ggcgtggtat cctggtgccg
     7261 tgtcggtttg caacttcgct cggtgggaaa gtatgcaccg cctttctctc tcgcccctct
     7321 ggggagaggg ccggggtgag gggaacacaa cacgaagagt ttaactatgt catcatcccg
     7381 tccggtgttc cgctcgcgct ggctgcccta tttgctggtc gcgccgcagc tcatcatcac
     7441 cgttatcttt tttatctggc ctgcgggcga agcgttgtgg tactcgctgc aaagcgtcga
     7501 tccgtttggt ttctccagcc agtttgtcgg cctggataac ttcgtcacgc tgtttcatga
     7561 cagctactat ctcgactcct tctggacgac gataaaattc agcacctttg tcaccgtcag
     7621 cggtttgctg gtgtcgctgt tctttgcggc gctggtggag tacatcgtgc gcggcagccg
     7681 tttctatcaa accttaatgc tgctgcctta cgctgtggct cccgccgttg ccgccgtatt
     7741 gtggatcttc ctgtttaacc ccggtcgcgg gctgatcacc cattttctcg ctgagttcgg
     7801 ctacgactgg aaccacgcgc aaaacagcgg tcaggcgatg tttctggtgg tgtttgcctc
     7861 ggtgtggaag caaatcagct acaactttct gttcttctat gccgcgctgc aatccattcc
     7921 ccgttcgttg atcgaagccg cagccatcga cggtgcaggc ccgattcgcc gcttctttaa
     7981 gattgcgctg ccgcttatcg ctccggtgag tttcttcctg ctggtggtga acctggtgta
     8041 cgccttcttc gataccttcc cggtgatcga cgccgccacg tccggcgggc cggttcaggc
     8101 caccacgacg ctgatttata agatttaccg cgaaggcttt accggactgg atctggcttc
     8161 gtctgccgcg cagtcggtgg tgttgatgtt cctcgtcatc gtgctgacgg tggtgcagtt
     8221 ccgctatgtt gaaagcaagg tgcgttacca atgattgaga accgtccgtg gctgacaata
     8281 ttcagccata ccatgctgat cctcgggatc gcggtgatcc tcttcccgct gtacgtggcg
     8341 tttgtcgcgg cgacactgga taaacaagcc gtctacgccg cgccgatgac gctaatcccc
     8401 ggcacgcatc tgctggaaaa catccacaac atctgggtga acggggtagg cacgaatagc
     8461 gcgccgttct ggcggatgtt gcttaacagc tttgtgatgg cgttcagcat tacgctcggc
     8521 aaaattaccg tctcgatgct ctcggccttt gccattgtct ggtttcgttt tccactacgt
     8581 aacctcttct tctggatgat ttttatcacc ctgatgctgc cggttgaagt gcgtatcttc
     8641 ccgacggtgg aagtcatcgc caacctgaaa atgctcgaca gctacgcagg tttaacgctg
     8701 ccgctgatgg cctcggcgac cgccacgttt ctgtttcgcc agttctttat gaccctaccg
     8761 gatgaactgg tggaagccgc gcggatcgac ggcgcgtcac caatgcgttt cttttgcgac
     8821 atcgtttttc cgctgtccaa aaccaatctg gcggcgctgt ttgtgatcac ctttatctac
     8881 ggctggaacc agtatttgtg gccgttgttg attattaccg acgttgatct cggcaccacc
     8941 gtggcaggga ttaaagggat gatcgccaca ggcgaaggca caacggaatg gaactcggtg
     9001 atggcggcga tgttgttaac cctgatccca ccagtagtga ttgttttagt gatgcagcgt
     9061 gccttcgtgc gcggcctggt cgatagtgag aaataagatg gcaggactga aattacaggc
     9121 agtaaccaaa agctgggatg gcaaaaccca ggtcattaaa ccgctgaccc ttgatgtggc
     9181 ggatggcgaa tttatcgtga tggtcgggcc gtctggctgc gggaaatcga cgttattgcg
     9241 aatggttgcc ggactggagc gggtaacgac gggcgatatc tggatcgacc gcaagcgtgt
     9301 caccgaaatg gagccgaaag atcgcgggat tgcgatggtg ttccagaact acgcgcttta
     9361 tccgcatatg agcgtcgaag aaaacatggc gtgggggctg aaaattcgcg gcatgggcaa
     9421 gcagcaaatt gccgagcgcg ttaaagaagc ggcgcgcatt ctggagctgg acggcctgct
     9481 taagcgccgc ccgcgtgagc tatctggcgg tcagcgtcag cgtgtggcga tggggcgtgc
     9541 gattgtgcgt gagccggcag tgttcctgtt tgatgagccg ctctctaacc tcgatgccaa
     9601 gttgcgtgtg cagatgcgtc ttgaactgca acagctgcac cgtcgcctga aaacgacttc
     9661 actctacgtt acccacgatc aggttgaggc gatgacgctc gcccagcgag taatggtgat
     9721 gaacggcggc gttgccgaac agattggcac gccagttgaa gtgtacgaaa agcccgccag
     9781 cctgtttgtg gcgagtttta tcggcagtcc ggcgatgaat ctgctgaaag ggcgtgtgaa
     9841 taacgaaggt acgcatttcg aactggacgg cggtatcgcg ctgccgctaa acggtggcta
     9901 ccgtcagtat gctggacgta aaatgactct cggcattcgc ccggaacata ttacgctacg
     9961 ttcgcaggct gaaggtggcg tgccgctggt gatggacacg ctggagatcc tcggcgcaga
    10021 taacctggcg cacggacgct ggggcgaaca gaagctggtg ttgcgactgg cgcatcagga
    10081 gcgcccgacg gcaggcagca cgctgtggct gcatctgccg gaaaatcagc tacatctttt
    10141 tgatggtgaa acaggacaac gagtatgagt aactggcctt atccccgcat cgtcgcccat
    10201 cgtggcggcg gtaaactggc cccggaaaac accctggcgg caatcgatgt gggggcaaga
    10261 tacggtcata agatgatcga atttgacgcg aagttatcga aagatggcga gatcttcttg
    10321 cttcatgacg acaatctcga acgcaccagc aacggctggg gcgtcgcggg tgaactgaac
    10381 tggcaggatt tactgcgcgt ggatgcgggc agttggtaca gcaaagcgtt taaaggtgag
    10441 ccgctgccgt tgctttcgca ggtggcggaa cgctgtcgcg aacacgggat gatggcgaat
    10501 atcgaaatca aacccaccac tggcaccggg ccattaacgg gcaaaacggt ggcgctggcg
    10561 gcacgcgaac tgtggaccgg tatgacgccg ccgctgctgt catcgtttga gattgatgct
    10621 ttagaagccg cgcaacaggc agcaccggaa ctgccgcgtg gtttattgct tgatgaatgg
    10681 cgcgacgact ggcgagaatt gaccgcgcgg ctgggctgcg tctctattca tctcaatcat
    10741 aagttgctcg ataaagcgcg agtgatgcag ttgaaagacg ccggattacg gattctggtt
    10801 tataccgtca ataaacccca gcgcgcggca gagttgctgc gctggggcgt ggattgcatc
    10861 tgtaccgatg cgattgacgt gattggtccg aactttacgg cccaatagtt ttcaacggaa
    10921 tgtcaggctg cggcgtgctg ggctgattca gcatgtcgcc gtttctcctt tccggcagca
    10981 tatgctgctg attcaacgaa ctatccggat tacggttgct gtttaacatc ccgccattgg
    11041 tgttgggcag catttgctgt cgggcgggat ttcgctcccc cggctgtgac tgcaacaccc
    11101 gctgagaatt attgtttatc tggttttcta aatgctgctg ttgcagctgc gtttgcgttt
    11161 tcagttgctg attcagcatc cctttttgct ggatttgctg cgtttgcatt tgggtttgca
    11221 tccgctgctg actggggatc tgataccccg gctggttagg gttattcaga gtattaatgg
    11281 gctgtgcaaa gccgacaaac ggcaggagtg ccgtaagaat cagaagtcgt ttcatcgcgt
    11341 atcctcctct gaagatattc tttaagttta ctcctttcct gcaatcatca ggattaattc
    11401 agagttatat accaggctta gctggggttg ccccttaatc tctggagaat aacgatgata
    11461 aaaccgacgt ttttacgccg ggtggccatt gctgctctgt tcacaggaag ttgttttagt
    11521 acggtagccg cgccacctgc gccgtctcct gtttcttacg gcgtggagga agatgtcttt
    11581 catccggtac gcgcgaaaca ggggatggtg gcgtctgtag atgctactgc cactcaggtg
    11641 ggggtggata tcctcaagga gggcgggaat gccgttgatg ccgccgtggc tgtgggctac
    11701 gcgctggcgg taacgcatcc gcaggcaggg aatctgggcg gtggtggttt tatgctgatc
    11761 cgctcgaaaa atggcaatac caccgctatc gatttccgcg aaatggctcc cgccaaagcg
    11821 acccgcgata tgttccttga tgatcagggt aacccggaca gcaaaaaatc actcacttcg
    11881 catctggctt ctggtacgcc cggtacggta gcaggtttct cgctggcgtt ggataaatac
    11941 ggcaccatgc cgttgaacaa agtggtgcaa ccggcgttta aactggcacg cgatggtttt
    12001 atcgttaacg acgcgttggc tgacgatctc aaaacctacg gtagcgaagt gttgccgaat
    12061 cacgaaaaca gcaaggctat cttctggaaa gagggcgcgc cgctgaaaaa gggcgacaag
    12121 ctggtgcagg cgaacctggc aaagagcctg gagatgattg ccgaaaacgg cccggacgaa
    12181 ttctacaaag gcacgatagc ggaacagatt gcccaggaga tgcagaaaaa cggtggcttg
    12241 atcactaaag aagatttagc ggcgtataaa gcggtcgaac gtacaccgat tagcggcgaa
    12301 tatcgcgggt atcaggttta ctcgatgcca ccgccatctt ccggtgggat tcatatcgtg
    12361 cagatcctca atatcctgga aaacttcgat atgcagaaat acggctttgg cagcgccgat
    12421 gcgatgcaaa tcatggcaga agcggagaaa tacgcctacg ccgaccgctc ggaatatctt
    12481 ggcgatccgg attttgtcaa agtcccgtgg caggcgctga ccaataaagc ctatgccaaa
    12541 tctattgccg atcaaatcga tatcaataaa gcgaagccat ccagcgaaat tcgccctggc
    12601 aagcttgcgc cttatgagag taatcaaact acccattact cagtggtgga taaagatggt
    12661 aacgcggtgg cggtgaccta tacgctgaac accaccttcg gtacgggcat tgtcgcgggc
    12721 gagagcggta ttctgcttaa taaccaaatg gatgatttct ccgccaaacc gggcgtgccg
    12781 aacgtttacg ggctggtggg tggtgacgct aacgcggtcg ggccgaataa acgtccgctg
    12841 tcgtcgatgt caccaaccat tgtggtgaaa gacggtaaaa cctggctggt caccggaagc
    12901 ccaggcggta gccggatcat cactaccgtg ctgcaaatgg tggtgaatag catcgattat
    12961 ggtatgaatg tcgccgaagc gaccaatgca ccgcgtttcc accatcagtg gttgccggat
    13021 gagctgcgtg tcgagaaagg atttagcccg gatacgctca agctgctgga agcaaaaggt
    13081 cagaaagtgg cgctgaaaga ggcgatgggc agtacacaaa gcattatggt tgggcctgac
    13141 ggtgagttgt acggcgcatc cgacccgcgc tcagtagatg atttaacggc ggggtattaa
    13201 ggttagcggc cctcttcgtg ggaagagggc cgtattgtca gggcaagccg aaggtagcct
    13261 tttttatttc ataatcctgt agatattctt ccagcggctt tgccgttcca agggaatgaa
    13321 tttcaccact gtctttatca ataataaaag gtgcgttacc ggctaagcgc gcggcctcat
    13381 caccagattc aagaaattct tgtgattcga aacagaaata ccagccctgg ataaaacgtc
    13441 catgcagagt gatgacgaca gggagatctg catcatcaag gtaatggttc gctttcgcga
    13501 atgcgtcgtg ataagtaatc ataattataa taaatattat tgttgagcgt tatattatta
    13561 ttcttgcgtt atactttgag aaaaggcgtg ttcatcttcg tcaaagatat tattaaatgc
    13621 t
//
LOCUS       DAZUCC010000136        13579 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZUCC010000136 DAZUCC010000000
VERSION     DAZUCC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.137"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            119..1486
                     /gene="dcuC"
                     /locus_tag="ACHY09_003398"
     CDS             119..1486
                     /gene="dcuC"
                     /locus_tag="ACHY09_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFS3886327.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCIIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTLFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAIGAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1530..2027)
                     /gene="sspB"
                     /locus_tag="ACHY09_003399"
     CDS             complement(1530..2027)
                     /gene="sspB"
                     /locus_tag="ACHY09_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFS3886328.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2033..2671)
                     /gene="sspA"
                     /locus_tag="ACHY09_003400"
     CDS             complement(2033..2671)
                     /gene="sspA"
                     /locus_tag="ACHY09_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFS3886329.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3139..4872)
                     /gene="ltrA"
                     /locus_tag="ACHY09_003401"
     CDS             complement(3139..4872)
                     /gene="ltrA"
                     /locus_tag="ACHY09_003401"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS3886330.1"
                     /translation="MIKLEENSHEYGKPLGPASSGHLSNWHNINWAKVTKVVRGLQVR
                     IAKATQDSDWRRVRNLQRKLTHSSCAKALAVRRVTENQGKRTAGVDRELWKTPESKWK
                     AIGQLTDKGYKPKPLRRVYIPKPDGKKRPLGIPTMKDRAMQALHLLALQPVAETMGDT
                     GSYGFRINRSTADAITQCHMTLNKAGSPKWIMDADIKGCFDHISHEWLLKHIPMNKRI
                     LNRWLKAGVVDMGELKKTEEGTPQGGIISPTLANMALDGLEKRLAEHFGEKASRRIRE
                     TKVYLIRYADDFVITGISQELLQQQVKPVVEAFLAERGLQLSETKTQTVHIDKGFEFL
                     GWMVRRYRGHILIKPSRKNEKTFYRNIKATITKHAAVGQEILIWKLNSKIRGWTNYHR
                     HQVASKAFGRMDTLIWKALWRWASRRHPNKGKKWVKNKYFHETSSRNWVFGTQVFGEN
                     GMEWLNLLYAGDVKIKRHQKVRANYNPFLPEWELYGEELSRKRLLDNQTHRRQWQFLY
                     HHQKGRCALCGQLITAQTGWHDHHIIYRVNGGKDTLENRVLVHPVCHAKIHALKLEVV
                     KPTAKEVNRKA"
     gene            complement(6082..6474)
                     /gene="rpsI"
                     /locus_tag="ACHY09_003402"
     CDS             complement(6082..6474)
                     /gene="rpsI"
                     /locus_tag="ACHY09_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS3886331.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(6498..6926)
                     /gene="rplM"
                     /locus_tag="ACHY09_003403"
     CDS             complement(6498..6926)
                     /gene="rplM"
                     /locus_tag="ACHY09_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS3886332.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(7145..8272)
                     /gene="zapE"
                     /locus_tag="ACHY09_003404"
     CDS             complement(7145..8272)
                     /gene="zapE"
                     /locus_tag="ACHY09_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFS3886333.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYDIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            8466..8864
                     /gene="zapG"
                     /locus_tag="ACHY09_003405"
     CDS             8466..8864
                     /gene="zapG"
                     /locus_tag="ACHY09_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFS3886334.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            9018..10385
                     /gene="degQ"
                     /locus_tag="ACHY09_003406"
     CDS             9018..10385
                     /gene="degQ"
                     /locus_tag="ACHY09_003406"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFS3886335.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            10475..11542
                     /gene="degS"
                     /locus_tag="ACHY09_003407"
     CDS             10475..11542
                     /gene="degS"
                     /locus_tag="ACHY09_003407"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFS3886336.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(11605..12543)
                     /gene="mdh"
                     /locus_tag="ACHY09_003408"
     CDS             complement(11605..12543)
                     /gene="mdh"
                     /locus_tag="ACHY09_003408"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFS3886337.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            12978..13448
                     /gene="argR"
                     /locus_tag="ACHY09_003409"
     CDS             12978..13448
                     /gene="argR"
                     /locus_tag="ACHY09_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFS3886338.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         3265 a         3496 c         3241 g         3577 t
ORIGIN      
        1 atctacaatt aaaaacacga catccgcacc ataaatagcc ttgcaaaaaa tataacatca
       61 ttgttttcaa tctgccgttt atgggattga ccattttctt ttgacacgga gttcaacaat
      121 gttcggcata attatatctg tcatcgtatt aattacgatg ggctatttga tcctgaaaaa
      181 ctacaaacct caggtggtgc tggctgccgc aggtatcttc ctgatgatgt gcggtgtctg
      241 gttagggttc ggtggtgtgc tcgatcccgc caaaagcagc ggctacttga tcgtcgatat
      301 ttataatgaa atcctgcgca tgctgtccaa ccgcattgct ggattggggc tgtcgattat
      361 ggcagtgggc ggttatgccc gctatatgga gcgcataggg gcaagtcgcg cgatggtgag
      421 cttgttaagc cgcccgttaa aactcattcg ctcgccgtat attattctgt cggcaactta
      481 cgtcatcggc caaatcatgg cgcagtttat taccagcgcc tccggtctgg gtatgttgct
      541 gatggtcacc ttatttccga cgctggtgag tctgggagta agtcgtctct ctgcggtggc
      601 ggttatcgca accaccatgt ccattgagtg ggggattctg gaaacgaact ccatttttgc
      661 tgcccaggtc gcaggaatga aaattgccac ttacttcttc cactaccagc ttccggtcgc
      721 ctcttgcatc attatctcgg tggcgatctc ccactttttc gtgcaacgcg ctttcgacaa
      781 aaaagataaa aatatcaatc acgaacaggc agagcaaaaa gctctcgata atgtcccgcc
      841 gctctattac gccattttac cggtgatgcc gttaatcctg atgctcggct cgctgttcct
      901 tgcccacgtc gggctgatgc agtcagaact gcatctggtg gtggtgatgt tactgagttt
      961 gacggtgacg ctgtttgttg agttcttccg caagcataac ttgcgcgaaa caatggacga
     1021 tgtgcaggcg ttttttgacg gcatgggtac gcagtttgcc aacgtggtaa cgctggtggt
     1081 cgcgggtgaa atatttgcga aaggcttaac gacgattggc actgtcgatg cggttatcag
     1141 gggggcggag cactctggtc tgggcggtat tggcgtgatg attattatgg cgctggtcat
     1201 tgccattggt gccattgtga tgggctcagg caatgcgccg tttatgtcat ttgccagtct
     1261 tattccgaat atcgcagccg gactacatgt accagcggtt gtaatgatta tgccgatgca
     1321 ttttgccacg acgctagcgc gcgctgtttc gccgattact gcggtggtgg tcgttacgtc
     1381 aggaattgca ggcgtttcgc cttttgcggt agtgaagcgg acagcgatcc ccatggcagt
     1441 cggtttcgtg gtgaatatga ttgccactat cacgctattt tattaagcga ttaaaaagac
     1501 aaaacaggcc gccagggcct gttttcattt tacttcacaa cgcgtaatgc cggtcgacca
     1561 ccgcgtggtg gctgcggagg ttcatcgtca ggatgagtgt catcatcgtg atctggcttg
     1621 tcgccatcaa taaccgacat aacggtttcg ttgtctgccg atgcctcttc atcattcatg
     1681 atgctggcat cttcatcgta ggcagcttca ggttcaaaca tcgtgcctgc gccattttca
     1741 cgggcgtaga tagccagcac ggcagccagc ggcacagaaa cctgacgcgg aatgccacca
     1801 aagcgcgcgt taaagcgcac ctcatcattc gccagttcca gattgccgac agctcgcggc
     1861 gcaatgttga gtacgatttg cccgtcacgc gcatattcca taggaacctg cacgccaggg
     1921 agcgtcacat ccaccaccag gtgcggcgtg agctggttat ccagcaacca ctcatagaat
     1981 gcacgcagca gatagggacg acgtggtgtt agctgtgaca aatccataca gattaactcc
     2041 ggcccagacg catttcacgt tcagcttcag ttaaagaagc aaggaaagag tcacgctcaa
     2101 agacgcgggt catatagcct ttcagctctt tcgcacccgg gccgctgaac tcgatgccca
     2161 gttgcggcag acgccacagc agaggagcaa ggtagcaatc gaccaggctg aactcatcgc
     2221 tcaggaagta cggcttctga ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt
     2281 gcttacgtgc ggcatcggct tcagaagctg aaccgttgat gatggtgttc atcagcgtgt
     2341 accagtcttt ttcgatgcga tgcatgtaca gacggctttc accgcgagct accgggtaaa
     2401 caggcatcag tggcggatgc gggaaacgct catccagata ttccataatg atgcgagatt
     2461 cccacagggt cagctcacga tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa
     2521 tcagatcctg aggcggattg tccttttcca cgtgttcgat ctcgaaactt acacctttct
     2581 cagccagcac aatgcggacc tgatggctat agatgtcagt aggaccggaa aacagcgtca
     2641 ttaccgaacg tttgttggca gcgacagcca tgaaaacctc caggtatagt cagaattttt
     2701 actgctacca gccaccaggt ggccagtcag aagttgtgtt acccagtaag gaacgactct
     2761 ctttgttcga aaatcaaaca aaaaatgagc aatacccgac atttgggcag aaaattggat
     2821 gatagtttac cagattttgc gacctttgtg gtgagtcgat gccggaaatg gggaaaaaga
     2881 gatgcgcttt agtctgaaat agttgactta gtcccttatt ggcgatgtgg tttttgtttt
     2941 acctgtctgt caggtggcag caaaaagcaa cttcccattt tttacgctga tgcagatttt
     3001 agccataaaa aaacccgccg aagcgggttt tttcgcaaat tgttttctgc cggagcagaa
     3061 gccaagtcgg gtgggcgacg tccattactg gacctcgccc ccctaagaac cgtacgtgcg
     3121 aatctctccg catacggctc aagcctttct attaacctcc ttagcggtcg gcttcacaac
     3181 ttctagtttc agtgcgtgaa ttttggcatg acataccgga tgtaccaaca ctctattctc
     3241 cagtgtgtct tttccgccgt tcactctgta aatgatatgg tggtcatgcc atccggtctg
     3301 tgccgtgatg agttgcccac ataaagcaca tcttcctttc tggtgatgat ataaaaattg
     3361 ccactgccta cggtgtgttt gattatccag taaccgcttc cgtgacagtt cctcaccata
     3421 tagttcccat tcgggtaaaa acggattgta attcgccctt accttctggt gccgctttat
     3481 cttgacatcg cctgcgtata gcagattcag ccattccata ccattctctc cgaagacctg
     3541 tgtaccaaat acccaatttc tgctcgaagt ttcgtgaaag tatttatttt tcacccactt
     3601 cttgcccttg ttggggtgtc ttcggctcgc ccatctccac agcgctttcc aaatgagagt
     3661 gtccattcgt ccaaacgctt tactggctac ttgatgtcga tggtagttag tccatccgcg
     3721 tatcttggaa ttgagtttcc atatcaagat ttcctgtcca accgctgcat gttttgttat
     3781 cgtcgcctta atgtttcggt agaatgtctt ctcattcttc cttgagggct tgataagtat
     3841 atgcccccgg tatctacgga ccatccatcc tagaaattcg aaccccttat cgatatgtac
     3901 ggtctgtgtt ttcgtctctg aaagctgtag acctcgctca gcaaggaatg cttccactac
     3961 cggtttcact tgttgttgta acaactcctg tgatatccct gttatgacga aatcgtcggc
     4021 gtaccttatc agatacactt tggtctccct tattctgcga ctagctttct caccgaagtg
     4081 ttcagccaat cgtttctcta ggccatctaa cgccatattc gccagtgtag gggagattat
     4141 tcctccttgt ggtgttcctt cttccgtttt cttcaactcg cccatatcaa ccacacccgc
     4201 cttaagccac ctattcaata tccgtttgtt cattgggata tgtttcagta gccattcgtg
     4261 gcttatatgg tcgaagcatc ccttaatgtc tgcatccatg atccatttag gggatcccgc
     4321 cttgttcagt gtcatgtgac attgagttat agcatcggct gttgaacggt ttatacggaa
     4381 tccatatgat cccgtatccc ccattgtttc agccacgggt tgaagtgcta gaaggtgaag
     4441 tgcctgcatt gcacggtctt tcatggtcgg aatgcccaac gggcgtttct ttccatccgg
     4501 ttttggaatg tataccctcc gcaaaggctt gggtttatat cccttgtccg ttaactgtcc
     4561 aatagctttc cacttcgatt caggcgtttt ccagagttct cgatcgacgc ctgccgttcg
     4621 tttgccctga ttttccgtca ctcgccttac tgccaacgct ttcgcgcatg acgagtgggt
     4681 caacttcctt tgcagattcc tgactctgcg ccagtcgcta tcctgtgttg ccttcgcaat
     4741 ccgtacctgc aatcctctca ccacttttgt aacctttgcc caattaatat tgtgccagtt
     4801 actcaagtga ccggaggatg ctgggcctaa aggtttccca tattcgtggg agttttcctc
     4861 tagcttaatc atgctttcct cctgtgaaac gggagtgagc agatttctcg gtaagatatt
     4921 ccagtttaca tcacctgaaa tagggtttat ctccgtccgt ccatttacaa ggcagacctg
     4981 acaaaccatc tgtattacca atagtctgtt cactgcctgt aacatggttg ttcgtgatta
     5041 ggcatctggt cgattggatt ctatccttcc aatctctagc atgtcttccc cactcaaacc
     5101 acagagaatt tgcctgccat cttacgatga aagaccagac ggaagtctgc accctttcgg
     5161 gtacgggtat gtttcatccc gttatccgct ccattacaga gcggcattcg ctttctccgt
     5221 cctcccttac ctgcacctcc aacaatttcc cttgcgggtc atctgccttt acaggcagag
     5281 atacaggctt accgagttcc ataatcgtca cacgaaaggt taagacgctg tctttttcac
     5341 cggcggttat tttggtgacg tatcccaacg ttaaaatggg ataaccaacc acataccttt
     5401 tggtcagagc ctatcagcaa ctttggctct ttctccttta cgatgtttaa acagacagtt
     5461 cactcacgtt cgtctttcct ctcagccttg cccctcaacc actttgttgc tagtagtatc
     5521 cacatgccta tctcacaata aacatgtacc agttatgggg gtacgttgtc aggaagctcc
     5581 gcacaaaacc gttaccagta ttgcactatc cctaggctac cgctggtcac acaacgggtt
     5641 gactacccac tctatcgggt ccaacaatga cgactacagc ttatgccctt gcattgcaag
     5701 tatctggaaa ggttacttca agacttagcc ctacgattta atcatcacat ttacagttct
     5761 atgacggtta agtaaggtag atctatcagt caacctagat aggtctctcc agctttcatg
     5821 attatctgaa aactgtgatt atttatgata caagacgagt tatcttgtat gaatatttaa
     5881 atatcctagg gcagtggtca ggtccacttg gataaaaccc ttttaacagt tactactttg
     5941 caaggcccct aggggccagc aagtagcact gaccaaaagg ggcttgcaat gcgttgaaag
     6001 acatgatagc gtgcctttca actctcctgg aaatcgttga aaaacaacaa cttccagagc
     6061 caagagcgac tctcgtcgca cttaacgttt ggagaactgc ggacgacgac gtgctttacg
     6121 cagaccgact ttcttacgtt caacctgacg agcgtcacga gtaacgaagc cagctttacg
     6181 cagttcagaa cgcagggact cgtcgtattc catcagagcg cgggtgatac cgtgacggat
     6241 cgcaccagcc tgaccagaga taccaccacc tttaacggtg atgtacaggt ccagtttctc
     6301 aaccatgtcg accagttcca gcggctgacg aactaccatg cgagcagttt cacgaccaaa
     6361 gtactgttcc agagaacgtt ggttgattac gattttaccg ttgcccggtt tgatgaaaac
     6421 gcgagctgcg gaacttttgc ggcgaccagt gccgtagtat tgattttcag ccattgccta
     6481 taatcccgat tagatgatta gatgtcaaga acttgcggtt gctgtgccgc gtggttgtgc
     6541 tcgttacccg cgtaaacttt cagtttacgg aacatagcac gacccagcgg gccttttggc
     6601 aacatgcctt taaccgcgat ttcaatcaca cgctcaggac ggcgagcaat catctcttca
     6661 aaggtcgctt gtttgatacc accgatgtgg ccggtgtggt gatagtacac tttgtcagta
     6721 cgcttgttgc cggttacagc aactttgtca gcgttcagaa cgatgatgta atcaccggta
     6781 tctacgtgcg gagtgtattc cgctttgtgc ttaccgcgca ggcgacgagc cagttcagta
     6841 gccagacggc ccagagtttt accggtcgcg tcaacaacat accagtcgcg ttttacggtt
     6901 tctggtttag ctgtaaaagt tttcattaaa agcttaccca ataaaaagtt acacgttggt
     6961 gaacacccaa acgtcttcaa ttgttgaggt tcacacgaca aagtccggca aacctacccc
     7021 ttcgaatagc ctatgccagc acacaaaaag ttttgggaaa aaaactttct tgtaacgtgg
     7081 ggtcgcagga ttatagagaa gtcggggtca aagatcgacc cctttttgtg atttgtggca
     7141 ggttttaacc cgccaaatgc tcgcgcttca gatactcttc gctttgcatc tcttgcagac
     7201 gtgacaggca acgctggaac tcaaatttca gccgctcgcc ctgataaatg tcatatagcg
     7261 gcacttctgc actcaccact aatttgacat ggcgctcgta aaactcatcc accagcgcaa
     7321 taaaacgtcg cgcttcgctc tccatcaacc gcgtcataac tggtacatca aacaacatga
     7381 ctgtgtgaaa gaggcgtgag agcgcaatat agtcatgctg actgcgggcg tcgacgcaca
     7441 gcgtagtaaa agagaccgcc agcgtctggt tctcgacgcc cattgtcgcc agcggccgat
     7501 ggttgatttc taatgtcggt gaattttctc gtttcgcccc cgccagcgcc aaccatagtt
     7561 tatccatttg cgcccgggtt tcatcgttga ggggcgaaag ccacagatgc gcctgagtga
     7621 gtgtacgcag tcgataatca acaccagcgt ccacgttcat tacatcacaa tgctgtttaa
     7681 tggcatcgat tgcaggcaaa aaacgcgcac gttgtaggcc atttcgataa agttcgtccg
     7741 gcggaatatt tgacgtcgct accagggtaa taccgcgggc gaacagggct ttcatcagac
     7801 cgccaagtag catggcatcg gtaatatcag aaacaaaaaa ttcgtcaaaa cagagcacgt
     7861 cagtttctgc tttaaagcga tcggcaataa tttccagcgg atcggtctgc ccctgcaaag
     7921 cagttagctc ttcatgtacc cgcagcataa aacggtgaaa gtgcagacgc tgtttccgct
     7981 ctcccggcag gctttgatag aaaaggtcca tcagccaggt tttcccgcgt cctacaccgc
     8041 cccacatata taagccacgc actggcgtat gctttgtgtc ttcgcgttta ccccacagct
     8101 taccgacccg cgccattagc ccactcgtcc tgggggctgg aggcgtgcta ttgatgagtt
     8161 cctgataaat aatttccagg cggctgacgg cctctttttg aacgtcgtcg ggttgatggc
     8221 tgccttcatt gagcgccttc aggtattgcg atgttggggt aacgctttgc atgatcttat
     8281 tgttattcct tgaataatcg gtgcgccgtt gttcacggtt gacgaaaaaa aggccgttct
     8341 acactacgcg atatgcagtc gggattccac ttctgtggaa ttaacggtta tagtggcata
     8401 atcagccgca ggcatggagc ctgaagccaa caccctacgg aaacaaaaga caacgggaga
     8461 tgttcatgac ctgggaatat gcgctaattg ggttagtcgt cggcatcatt attggtgctg
     8521 tggccatgcg ttttggtaat cgtaaactac gccagcaaca ggcgttgcag tacgaactgg
     8581 aaaagaataa agctgaactg gacgagtatc gcgaagagct ggttagccac tttgcccgca
     8641 gcgcggaatt actggatacc atggcgcacg actatcgcca gctgtatcaa cacatggcga
     8701 aaagctccag cagcctgctg ccggaactgt ctgctgaagc aaacccgttc cgtaatcgtc
     8761 tggcagagtc tgaagccagc aacgatcagg caccggtgca aatgcctcgc gactattctg
     8821 aaggcgcatc cggcctactg cgtactggcg cgaagcgcga ctaatttatt tttcgggcgc
     8881 agccattgcg ccctcctctt ctctccctcc ccgactatca tttaatctgg tgtctcattg
     8941 ttagccgtct gaaaattcaa taacatcaaa ctgttttgaa tctcttttct tatcattcag
     9001 gtacgagagt aggaataatg aaaaaacaaa cccagctgtt gagtgcatta gcgttaagtg
     9061 tcgggttaac tctctcggcg tcatttcagg ccgttgcgtc gattccaggc caggttgccg
     9121 atcaggcccc tctccccagt ctggccccaa tgctggaaaa agtgcttccg gcagtggtga
     9181 gcgtacgggt ggaaggaacg gccagtcagg gacagaaaat cccggaagaa ttcaaaaagt
     9241 tttttggtga tgatttaccg gatcaaccgg cacaaccctt cgaaggttta ggctccggtg
     9301 tcatcatcaa cgccagtaaa ggctatgtgc tgactaacaa ccatgtgatt aatcaggcac
     9361 agaaaatcag tattcagctc aatgatgggc gcgagtttga tgcaaaactg attggtagcg
     9421 atgaccagag cgatatcgcc ctgttgcaaa ttcaaaaccc gagcaaatta acgcaaatcg
     9481 ctattgccga ctccgataaa ttgcgcgtcg gtgattttgc cgtggcggtc ggtaacccat
     9541 ttggccttgg gcaaaccgcc acctctggca ttgtttccgc attaggccgc agtgggttga
     9601 atcttgaagg tctggaaaac tttatccaga cagatgcttc cattaaccgc ggtaactccg
     9661 gcggtgcact gttaaatctt aacggtgagt taattggcat caacacggca atccttgcgc
     9721 ctggtggcgg gagcgtcggg attggatttg ccatccccag taatatggcg cgaacactgg
     9781 cgcagcagct tatcgacttt ggtgaaatca aacgcggtct gttaggcatc aaaggcacag
     9841 agatgagtgc cgatatcgcc aaagccttca accttgacgt gcagcgtggc gcgtttgtca
     9901 gcgaagtgtt gccaggttct ggctcggcaa aagcgggcgt caaagcgggc gatattatta
     9961 ccagcctcaa cggcaaaccg ctgaatagct ttgctgagtt gcgctctcgt atcgcaacca
    10021 ccgagccggg cacgaaagtg aagcttggcc tgctgcgtaa cggcaaacca ctggaagtag
    10081 aagtgacgct cgataccagc acctcttcgt cggccagcgc tgaaatgatc acgccagcgc
    10141 ttgaaggtgc aacgttgagc gatggtcagc taaaagatgg cggcaaaggt attaagatcg
    10201 atgaggttgt caaaggaagc ccagctgctc aggcaggctt gcaaaaagac gatgtgatca
    10261 ttggcgtcaa ccgcgatcgg gtgaactcga ttgctgaaat gcgtaaagtg ctggcggcaa
    10321 aaccggccat catcgccctg caaattgtac gcggcaatga aagcatctat ctgctgatgc
    10381 gttaatgtcg taaaccgggc atcaggctga cgtgtgatgt ccggttaact cgtggtatgc
    10441 tgctgccgtt ccctttttta atgacgcctc catcatgttt gtgaagctct tacgttccgt
    10501 tgcgattgga ttaattgtcg gcgctattct gctggttgcc atgccttcgc tgcgcagcct
    10561 taacccgctt tccactccgc aatttgacag taccgatgag acgcctgcca gctataatct
    10621 ggcggttcgc cgcgccgcgc cagcggtggt taacgtttac aaccgtggtt tgaacaccaa
    10681 ctctcacaac cagcttgaga tccgcaccct gggatccggt gtaatcatgg atcaacgcgg
    10741 ttatatcatc accaataaac acgtcatcaa cgacgccgat cagatcatcg tcgccttaca
    10801 ggatggacgt gtttttgaag cgttactggt tggctctgac tctctaaccg atctggcggt
    10861 gctgaaaatt aacgccacag gcggtttacc aacgattccg atcaatccac gccgcgtacc
    10921 gcacattggc gatgtggttc tggcgatcgg taacccgtac aacctcgggc agaccattac
    10981 ccaggggatt atcagtgcca cgggtcgaat cggtctgaac ccgaccgggc ggcaaaactt
    11041 cctgcaaacc gatgcttcca ttaaccacgg taactctggc ggcgcgctgg tgaactcgct
    11101 gggcgaactg atgggcatta acacgctgtc gtttgataag agtaacgatg gcgaaacgcc
    11161 ggaaggtatc ggctttgcga ttcctttcca gttagcgacc aaaattatgg ataaactgat
    11221 ccgtgatggc cgtgtgatcc gtggctacat tggcattggt ggacgtgaga tcgccccatt
    11281 acatgcgcag agtggcggca tggatcaact gcaagggatc gtggttaacg aagtgtcgcc
    11341 tgatggccca gcggcaaatg caggcatcca ggtcaacgat ctgattatct ctgtgaataa
    11401 caaacccgct atttccgccc tggagacaat ggatcaggtc gcggaaattc gccctggttc
    11461 ggtgatcccg gttgtcgtga tgcgtgacga taaacagtta acattgcagg tcaccattca
    11521 ggaatatccg gcgaccaact aaggtcagcg taaaaaaaca aaaaccggag tctgtgctcc
    11581 ggttttttat tatccgctaa tcaattactt attaacgaac tcttcgccca gggcgatatc
    11641 tttcttcagc gtatccagca taccttccag cgcgttctgt tcaaatgcgc tcagggtacc
    11701 gatagattta cgctcttcca cgccgttttt acccagcagc agcggttgag agaagaaacg
    11761 tgcgtactga ccgtcgcctt caacgtaggc acattcgaca acgccttgtt cgccctgcag
    11821 tgcgcgaacc agagacagac caaaacgtgc agctgcctgg cccatagaca gggttgcaga
    11881 cccgccaccg gctttcgctt caaccacctc agtacccgcg ttctggatac gtttggtcag
    11941 atcagccact tcctgctcgg taaaactaac gccaggaacc tgtgacagca gcggcagaat
    12001 ggtaacacca gagtgaccgc caataaccgg cacttcaact tcgcctggct gtttgccttt
    12061 cagttccgca acaaaggtgt tggaacgaat gatatccagc gtggtaacgc cgaacagttt
    12121 gtttttgtca taaacaccgg cttttttcag cacttcagca gcaatcgcaa ctgtggtgtt
    12181 aaccgggtta gtgataatac caatgcacgc tttcgggcag gttttcgcaa cttgctgtac
    12241 caggtttttc acgatgccgg cgttaacgtt aaacaggtcg gaacgatcca tacccggttt
    12301 acgcgctaca cctgcagaga taagaacgac atctgcgcct tccagcgccg gagtcgcatc
    12361 ttcaccagaa aaacctttga ttttcacagc agtagggata tggctcagat cgacagccac
    12421 accgggagtc actggagcga tatcatacag agagagttct gaacctgaag gcagttgggt
    12481 ttttaacagt agtgcaagcg cctggccaat accgccagca gcgccgagga ctgcgacttt
    12541 catcctaaac tccttattat attgataaac taagatatgt tgctccgctg ccgcgacctt
    12601 aatccacaaa attgccgttt acaatgacca catctcaaga atgtgtagtc acgcaagttt
    12661 agcgtttatg catttaattg ccgtaatcag gaacctaact tacgtaatta acagtcgctt
    12721 atgcattagc gcaacattcc aacaggtggt gacaatatac cctaccgttc agccaaaaca
    12781 acatcaattt gataacaatt aatttacttt taagcagaat ttgcatgccg tgacgcaggc
    12841 atgtttctca ataacgaaat ttgataaaat cccgctcttt cataacatta tttcagcctt
    12901 cttcagggct gactgtttgc ataaaaattc atctgtatgc acaataatgt tgtatcaacc
    12961 accatatcgg gtgacttatg cgaagctcgg ctaagcaaga agaactagtt aaagcattta
    13021 aagcattact taaagaagag aaatttagct cccagggcga aatcgtcgcc gcattgcagg
    13081 agcaaggctt tgacaatatt aatcagtcta aagtctcgcg gatgttgacc aagtttggtg
    13141 ctgtacgtac acgcaatgcc aaaatggaaa tggtttactg cctgccagct gaactgggtg
    13201 taccaaccac ctccagtcca ttgaagaatc tggtgctgga tatcgactac aacgatgcag
    13261 ttgtcgtgat tcataccagc cctggcgcgg cgcagttaat tgctcgcctg ctggactcac
    13321 tgggcaaagc agaaggtatt ctgggcacca tcgctggcga tgacaccatc tttactaccc
    13381 ctgctaacgg tttcaccgtc aaagacctgt acgaagcgat tttagagctg ttcgaccagg
    13441 agctttaatc tctgccccgt cgcttctggc ggcggggaaa atgttgcttt tccctctcac
    13501 cccccctgct ttctcctgcg attaatttaa cgaatagtgc gttttatcgc gacatatcat
    13561 tcacataatg aatacatgg
//
LOCUS       DAZUCC010000137        13482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZUCC010000137 DAZUCC010000000
VERSION     DAZUCC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.138"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..4904)
                     /locus_tag="ACHY09_003410"
     CDS             complement(<1..4904)
                     /locus_tag="ACHY09_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFS3886339.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGVAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGV
                     ATVQDIFAGGSRGADEVIDTLPDGKNVVTDTVGKGLKATGKAVSDGAKASDEWLTEQM
                     SPGAVRALNTPITEGYDDSAVLLAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTAG
                     LEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNA
                     DYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVN
                     AMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGVINGAQNAYSRYQENMALRETAG
                     MDVSPWEGVTDAGIEGTVFGAAMGAPFGAVAGYRGRRQAAEEAAMRDAETVQQDDAAP
                     QPEPVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQA
                     KRQAVMNELKAKPRSELLEEYRRLSQKEERTETEEQQFQAIREVIRPQQEVTPEAQSQ
                     PENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGS
                     DVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPMEQ
                     PAFMRDPRFRGFTGDDTEVQARLARGNAPMAEELVRSQMAEGDAGPTAQELTERPRLP
                     APGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAPAPENAAGRQ
                     GETLEGEIVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELA
                     QQAEVRRQAGGNRDIPQPETIAPESETTVFTDRESTVRGGEVRGKKIEDFGEEIKGAA
                     KHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIP
                     AKTKHNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPP
                     SQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAKV
                     WFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASD
                     ARKYIDSHRDELVRHVKDMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRG
                     VQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAH
                     YEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRRI
                     FKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAAR
                     AFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTL
                     KTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAAEAKGSPVPQVEAVARGVMSGIKDS
                     DLKVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVL"
     gene            complement(4990..6252)
                     /locus_tag="ACHY09_003411"
     CDS             complement(4990..6252)
                     /locus_tag="ACHY09_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886340.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLAKLQEVASNVAADAIKNGRDAQAEVDEFYAANGLQ
                     PHQQKVQQRKTQQLLQKWQTWAAGDPVKQEFVKEAAANAPALLEPGREKEVEIYYQNH
                     LRDKKAEHEKALDYSASAAAEKIKGWMK"
     gene            complement(6263..6514)
                     /locus_tag="ACHY09_003412"
     CDS             complement(6263..6514)
                     /locus_tag="ACHY09_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS3886341.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            complement(6524..6970)
                     /locus_tag="ACHY09_003413"
     CDS             complement(6524..6970)
                     /locus_tag="ACHY09_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886342.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAV"
     gene            complement(6973..7629)
                     /locus_tag="ACHY09_003414"
     CDS             complement(6973..7629)
                     /locus_tag="ACHY09_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886343.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYENIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(7723..8076)
                     /locus_tag="ACHY09_003415"
     CDS             complement(7723..8076)
                     /locus_tag="ACHY09_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886344.1"
                     /translation="MQRVVRNSRNPEFMNEVKDACLKKQAFCFEAPDGFLVLRSVLSA
                     DGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHTARRGFIRLGKRLGFER
                     MPDDEDGFMVFRIAV"
     gene            complement(8212..9417)
                     /locus_tag="ACHY09_003416"
     CDS             complement(8212..9417)
                     /locus_tag="ACHY09_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3886345.1"
                     /translation="MKKASKLLLALMPALSLSVFSAGVYAADASGINGTSYYGVTVDQ
                     LKGHEYKTPIDFWRDLGFSGKGYLDGVVVNADTRKPESVNIQNTAGKYLKYSLYDVGK
                     SGLTNTELLAPRSEIASYYDYANEVAYFVNTKTNQILFSADGEGLVTTVGADGKAYSR
                     HIPEISEYIYEIKIKSVIPGGTIDSTLSAADVTDIHARLSSLGTGKTQVGGGATAGGE
                     NATAVGYNAIANHTNSVAVGANSQTTRPDEVNVGNRVIGGLRDGVQAADAATYGQLER
                     YRIAEENARIQGDAQTLKSANTYTDNRVSTLERSTNQQFRQLRDEVEKNRKRADAGIA
                     GAMAMTAIPVVEGKTYSFGMAASNYRDEQAIAAGMHFRTTENSAVRLNASWDTQNGSG
                     VAAGMSVGW"
     gene            complement(9769..9909)
                     /locus_tag="ACHY09_003417"
     CDS             complement(9769..9909)
                     /locus_tag="ACHY09_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3886346.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLYGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(10141..10875)
                     /locus_tag="ACHY09_003418"
     CDS             complement(10141..10875)
                     /locus_tag="ACHY09_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS3886347.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            complement(10966..11583)
                     /locus_tag="ACHY09_003419"
     CDS             complement(10966..11583)
                     /locus_tag="ACHY09_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886348.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRETNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            complement(11589..11867)
                     /locus_tag="ACHY09_003420"
     CDS             complement(11589..11867)
                     /locus_tag="ACHY09_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886349.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(11882..13150)
                     /locus_tag="ACHY09_003421"
     CDS             complement(11882..13150)
                     /locus_tag="ACHY09_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS3886350.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(13147..>13482)
                     /locus_tag="ACHY09_003422"
     CDS             complement(13147..>13482)
                     /locus_tag="ACHY09_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886351.1"
                     /translation="AKDMMRVVTGDKMSVLAGGSLPSTIRWHSKIFSLPERTSFSCIR
                     VKSPAPERVGITIMADDVPVIHFAPGTFKGSVVRLPAATGQNWQVMVSGFGQVERITL
                     STSMSEMPV"
BASE COUNT         3149 a         3903 c         2887 g         3543 t
ORIGIN      
        1 agtacccctt tgaccagggc aacaacacgc tcccatgccg ccacgaattt attcggctgt
       61 ttctccgcca tatgtgccag aaattcaccc gcctgtactt ccggtgattc cttaccatag
      121 gacgcatcaa ccttacgcca ggcttcacgg atggtggcgt tatcactgtc gcgggttttc
      181 agcacggttt tgataatcgt ctgatattcc gctggcgtga caacatgctc catggcatgg
      241 tggataatct cgtgacgcag cttctcgcgt acggtccgcc cgtcagggat gttatccgcc
      301 accaggacta tttctcgttt atccggacga tagaatgcgt gcaccctgcc gtaaccatcg
      361 aacgattcgc ccgccagcgc ttcagcctct ttctgtgact tcaccacacg gaccttcagg
      421 tcactgtcct taatgccgct catcacgcca cgggcaaccg cttcaacctg cgggaccggg
      481 ctgcctttgg cttccgccgc actacggtta acatccgaaa tgagattgcc ttcaggtgtg
      541 cgggttacgc ccttacggga ataaaacgca acgcccttgt ccgtctcacg ggttttcagc
      601 gtactgaaca ggtgatcgaa tgcatcacga ataccgccat ccagttccgc attcgtcgga
      661 taagcccagg tgttatctgt gttgtgctca ggtgccttac ggatattgac cagataatca
      721 ttctccacgc cagccatacg cgccttatcc tgaacataac gctcaaaggc acgcgccgcc
      781 atttcaacat ccgttgacca gtacggtttt gagcgcacct catcaagaag cgctgaacga
      841 cgcggcatgt cactgttttt aatggcctga atcacacctt taaaagcgtc gtaaacctcc
      901 tgacgtaccg gatattcagc atcaacatac ctgccgtctt taaatatgcg cctgacacgc
      961 tgtgcttccg tcataaagtc gccacctgac gtaattttcc cgtcattgga aacgtcataa
     1021 cgaccaaaat aattatccag agaatggaac cattcgtgcg ccagcgcacc cggtccgtta
     1081 ccttttgtca ggttgattgc cacctcaccc ggctcatagt gtgccgccgc cttaccctta
     1141 ccacgggcac caaatgccag gccaagacga ccgttcaggg aaagcgcttt tgtcggtaca
     1201 ttcagtactt ccgcaaggtc atgcagcgag tcataagccc ggttcaaatc agcctgacga
     1261 cgcggacctt ccacgtagtt accaaactgc acaccacgga aaccaaacgc atcactgaac
     1321 tgctccggtg aaacatcccc tttacggcgt tctggtccgg tacggtcgcg gttggtggcg
     1381 ttacgctgct cctcacgcga aatctcccgc atatccttca catgacgaac aagctcatca
     1441 cgatgtgaat caatgtactt acgcgcatca ctggctgact taaagccacc tctcacccgc
     1501 attttgtttt taccgtaagc gataaaaata tcgccactgc gggtattccg gtaaacgtca
     1561 aagcggattt tgtcatccgg tgacggtgct gttttttcat cacctttcgc ctgtgatttt
     1621 tcctcctgct ctgcaaacca gactttcgcc tttgccagta attcatccct gctttccgag
     1681 aaaaagaggt tactcccctt attgtcctta ttgcgcagtg aataaagttt ctgtggcgga
     1741 tcgtaacgct tccctcctgc cgcctgatac acacccgata ccacccgata agcagaagcc
     1801 ctgtccatct gtgagggtgg cagagtgcgt aacagttgcc aggtatccgc gtaacgggag
     1861 ggcattctgc cttccatcca ttctgcgagg cgtttcgcgc tgaccgttcc gttcagcatt
     1921 tccgatacac tgtgtcgtac tttttttacg ctctcccccc agcctgccgt attgtgtttc
     1981 gtcttcgccg gaatatcgct acgatacagc gctatcattg ccagagtgtc agcatcggca
     2041 ccttcgttcg ccagttttgc gtagtccggt ttcgggaaca gtttgctcag cggctgcgtg
     2101 gcataatccc tgtcttccag cgttttaccc agtgtttcag caagctgtgc ataacggtgt
     2161 ttggctgccc ctttaatttc ctcgccaaag tcttcaattt ttttgcccct gacttcacct
     2221 ccgcgcacgg tagattccct gtcagtaaag acagttgttt cagattcagg tgcgattgtc
     2281 tcaggctgcg ggatgtcacg atttcctccg gcctgtcggc gtacttctgc ctgctgtgcg
     2341 agctcaggca gtgagtcccg tacagtgcgg ggaagtgatt caggctgagg catacgaaca
     2401 ttacgcgcaa tgtcaggagc cggtagccct tcacgtaccg gtgctgtcgc gttctgcgca
     2461 tccggtgacg gcagaccacg acgaactatt tcaccctcga gtgtttcccc ctggcgacca
     2521 gcggcgtttt ccggtgccgg agcacgtcct ttctggaaac tctggccctt aacctcaccg
     2581 gttgtggtaa aacgaccacc acgtccggcc tgattctcat ccggcgtacg cgccacctct
     2641 cccggtaacg gatatccctg tccgggatga atatcgccgg gggcgggcag acgcggacgc
     2701 tcagttaact cctgtgctgt cggaccggca tcaccctcag ccatctgtga acgtacaagc
     2761 tcctccgcca tcggcgcgtt gccacgggca aggcgggcct gtacctccgt atcatccccc
     2821 gtgaaaccac ggaatcgcgg gtcacgcatg aacgcgggct gctccatcgg gtcagcatca
     2881 gcaatacgct ctccgtcagc cagggtgtta atggtttccg ttgccacatc ttcagagaac
     2941 gcaggattat ccatttcaac ggcatactga ttctcgcctt tcttcactac ggacggtttc
     3001 aggccggtag cggcggcatt acggaaaaca tcactcccca tggcgctttt ctcatccgtg
     3061 aaataacggt tgtccgggcg caccttttca atgcgttccg ctggtctgga cgcaccattc
     3121 ccgttaattt caatgcggac ttcattcggg tcccggaacc gcaccgtcgg gtaaatgctc
     3181 ccgttaccat cctccgcatt ttcaggctgt gactgtgctt ccggcgtcac ttcctgttgt
     3241 gggcgaatga cttctcgtat tgcctgaaac tgttgttctt cagtctcggt gcgctcctct
     3301 ttctggctga gtctgcggta ttcctcaagc agttcagaac gcggcttcgc cttcagctca
     3361 ttcatcacag cctgtcgctt cgcctgttcg tccagttcat tcaacaactg gctggcagct
     3421 tcccggcgat gagctgcgga tgcgtcaccc tctgttgcca tatccgcatc agcatactgt
     3481 tccagaagct gctcgcgatt catgccctgc atggattcac gatgctgtgc caccggatca
     3541 accggttcgg gttgcggggc tgcgtcgtcc tgctgcacgg tttcagcatc acgcatggct
     3601 gcttcttctg cggcctgacg tctgccacga tatccggcaa ccgcaccgaa tggagccccc
     3661 atcgcagcac cgaaaacagt gccttcaatc cctgcgtcag tcaccccctc ccatggcgat
     3721 acatccattc cggcggtctc acgcaatgcc atgttttcct gatagcgcga ataggcgttc
     3781 tgtgccccgt taattacgcc ctgttccgtg gcatttctga caatgccgct tttaacggtc
     3841 ttcgctgtgc ctcgtgtcac cagattaaac agttgtgcgt cacccagttt tgccgccatg
     3901 gcattcacag ccagcaattc aggatcggtt gccagctgcg cgcgcacctc atcggcaaca
     3961 cgctcttttg ccagatccat tttctggcga tcagtaagct gtgcatgctg cgggtcggcg
     4021 tcaatggaca aaaacgtctg ctgaaatttc ggtgactgcg ccagctcaga gtaatccgca
     4081 ttaagaacag catctgctgc cgccattgca ctctgcccct gtgcactggc tgtggaatgg
     4141 gtgatcaggc ccgcctggaa taaatccggc atttttttat cgacagcttc tgctgccagt
     4201 gccgtggctc tttccggctg catccctgcc gcgatgtatt ttttctccag cccggcggtc
     4261 agcatttttc gcagtgtgac atcacccacc tttctagcca caccgccagc aaccatatca
     4321 ggtacaagcg caccaatcag gtttacaccc ttcgccagca agaccgcaga atcatcatag
     4381 ccttctgtta tcggcgtatt cagcgcacga acagcacctg gcgacatctg ttctgtcagc
     4441 cattcatccg aggctttggc accatcactg acagccttac cggttgcttt cagaccttta
     4501 ccgacagtat cagtgaccac gttttttcca tcaggcaggg tatcgatgac ctcatcagcc
     4561 cccctgctgc caccggcaaa aatatcctga acagttgcga cacccggtag ccccatacgg
     4621 ctgaactcat ttaaaatacg cgcccctgtt tttaccgggc tctgaatcat cgcatcaccg
     4681 agtccacggg ccatttcccc tgttccccgg acggactggg caacaccttt acccattgtt
     4741 ggcaatacat caccgaggct gaacgatgtg ctgtgatcaa tggcattctg cgggttgctg
     4801 aaaaaatcct gccagtaatt ttctcccggc tgcttaatgt tcaggctgct gcggttctgg
     4861 ttacccaatt gcgcttcagg gcgctgctca tctgaaaaag ccatacagac tccagtaaaa
     4921 aaaagcccga cacgatggtc gggcgtaagt gaacgatatt aacggggagc gcgttactgc
     4981 cgcatatctt tacttcatcc agcctttgat cttctcggca gctgcggaag cgctataatc
     5041 cagcgccttt tcatgctctg cctttttatc ccggaggtga ttctggtaat aaatttccac
     5101 ctccttttca cgaccaggtt ccagcaaagc aggcgcattg gcggcagcct cttttacaaa
     5161 ctcctgttta accggatcgc ccgccgccca cgtctgccac ttctgtaaaa gctgttgcgt
     5221 tttccgttgc tgaactttct gttgatgcgg ctgtaaacca ttcgcagcat aaaattcatc
     5281 cacctcagcc tgagcatcac ggccattctt gatcgcatcc gccgctacgt tgctggctac
     5341 ttcctgaagt ttcgccagag aaagtccctg ctgcttcggc ataaaataac cataattttt
     5401 cgatatctct gcaaatttgc tgcgatccct gacctgagcg atagccttat caacgggtat
     5461 cgccagcacg gtttgatcat cagggtgcgc actgccgtat tctgttacag gtttatgcgc
     5521 ggtggagcca tcgctgtatg tgagatcaag gccaataagt acgtaccctt cctgctgcgc
     5581 cggtacgata ctgccaatcc tggcctcttt tatcgttttc tccccggttg aatcaggcat
     5641 gccaatacgc tgttgtagtt ccggggcaaa cacgccggaa agcacatcga gattttccgg
     5701 ggtattcagc gaagcaatcg ccctgtccgg cttatcatca aagattttct gcaggttgtt
     5761 cacggcctga cccgctttcg cagcatagcc taccgttgac atcatcttaa gcggattggc
     5821 gtccgataat ttaccgaaca gatgagaagc cgcatcatgg tcacccgcat caatcgcttt
     5881 tcccagcgcc gccatcacag gctgatcgcg cgccagcata tcgttatact caagccgtcg
     5941 ctggttgtat ttctgcaact ggagtcgttg ctgttccatc cccagcgacg cattccggta
     6001 attctggttg gcgttaaact ccctttcctg cagtgtgtaa ttgcggtcat caactttggc
     6061 tctgtaatca aagttccgct gatcaacgtc tttattatgt tcaaactgag actgcgcaaa
     6121 ttcaaaatcg cgctcgttat ttttctgttg ctgagcaagc tgtgcttctc gcaaaccaag
     6181 ctccttacga cgggtcattg cctggtcaac agtgctgaat ccggcaagta acccctgtgc
     6241 aaatccgctc attcaccact ccttaaaaca aagaaccagc aatgccgcca attactgcac
     6301 cagcaacagc gccaacagga ccaccaacgg atgcgccaat agccgccccc gtaccaatgc
     6361 ccgtaccgat attctgtttt ttctgagctt tctgttgagc agccagttgt ttatttgatg
     6421 cctcaatctc tgtgcgtcgt ctgtctgcgt cacttattcc ctgtaatgcc tcacgccgcg
     6481 actgatttgc aatatccagt aaaccgtatc ccatattgcc cccttacact gccaccagtt
     6541 gtccgccaac actcagtttc tgtcttgccg gtgcagaagc ccccgtcaga atattcatct
     6601 ggcgatcctg ttcggcttca cggataccat ttttcgcgcc agcaattgcc agggcattac
     6661 gtaaccccag cgtattactg tcgggattat ccgggcggct taccccgtat ctcgccatct
     6721 ggttatcctg cgccatctgc gctgtacgga gactggaagt ggcaaggccg cccacccggg
     6781 caagctgtgc attcatcagg ctgttgttct cgccaaggtc agccagcctt gccacgcggg
     6841 gcaaatatct ggttcgccag tcgtcgtatt gttggcgtgt cagcgctgct gacgtctgcc
     6901 agtcaccctg tggacgggca gctccggtat aaccgtatct cgcaagtgtt tcgtatttac
     6961 cgtactccat aatcacagtc tccagttctg agcctgatgc tgaatggcat tagcgccggt
     7021 gccaggtgtt ttagcaccgc cacttcctcc gctaccgcca gccttatgca tcgcatatgc
     7081 acctgccgca cccaaaccag cgccaacaag agaggctcgc ccctgctgtt tcgtaaacgc
     7141 agcctgtgcg tccgatttag cttttgccag actgctgtct gccagcgagt taaaactctg
     7201 taacgcatcc gccttctgac cagaaccgag agctgcaaca tcctgtagcc cggcaacata
     7261 cttatctgcc tgcgatacct gcccccgtgt ggttgtgtca atctgcccgg ttacctgatc
     7321 actctggttc gcattcatta ccgcgttaaa gcgaccactg gacggatcaa caccggactg
     7381 agcaagatta cccgccagct ccttccgtgc ttcgccaaac tgtttctgat aaccaagatt
     7441 tgttgtgcca gcgatatttt cgtactgctg ctcactgttc aggtcatcga ccttttccat
     7501 gaagttatct tctgccggac ggaggatttt ttgataatcc tgccaccctt tccaggccac
     7561 ttcttcctgc gctatttgcg ctgctgttgg ttttactttg gtatcaccac cgcctttact
     7621 tccacccata atggccccct ggataacaaa aaaccctgcc ggagcagggt cagaatgtga
     7681 attacaatgt tggtttaaac acaatgatga aaactgtagc agtcagaccg ctatcctgaa
     7741 caccatgaag ccatcctcat catccggcat tcgctcaaag cccagtcgtt ttcccagccg
     7801 gataaatccc cgccttgccg tatgaaattc agcccagcgt ccgccagcca gacgggttaa
     7861 tgtcttcacc tccggcagat aacgctcaac gctgttactc cccgtacaca cgcccagcaa
     7921 caccagaaca taagggatac catcagcact gagcacagaa cgcagcacca gaaagccatc
     7981 aggtgcctca aaacaaaacg cctgcttttt aaggcaggcg tctttaactt cattcataaa
     8041 ttcagggttg cgggaatttc tcaccacacg ctgcatatac cggagaattt tttcactcac
     8101 actctcacca caaaaaagaa caatgtgact gtgctgtgtc ttcagcgaca gaaggatgac
     8161 ggagaacgct tccgataacc aacacggctg gcgttctccg caaaaaataa attaccagcc
     8221 gacagacata cccgcagcaa cacctgaacc gttctgggta tcccatgaag cattcaaacg
     8281 tacagcagaa ttttcagtgg tgcggaagtg catacctgct gcgatagcct gctcatcacg
     8341 gtagttactt gcagccatac caaaggagta cgttttaccc tcaacaacag gaatggcagt
     8401 catcgccatt gctcctgcaa tacctgcatc tgcacgttta cggtttttct ccacctcatc
     8461 acgtaactga cggaattgct gattagtact acgctccagg gtactgaccc ggttatctgt
     8521 gtaggtatta gcagacttca gtgtctgtgc atcaccctga atacgggcat tctcttctgc
     8581 aatacgatag cgctctaact gaccataggt tgcggcatcc gcagcctgaa caccatcacg
     8641 aagcccacca attacacgat tccccacatt aacttcatcg gggcgagtgg tctgagagtt
     8701 agcccctaca gcaacactgt tcgtatggtt tgcaatggcg ttgtaaccga cagcagtggc
     8761 gttttctccg cctgccgttg ccccgccacc aacctgagtt ttaccggtgc ccagagaaga
     8821 cagacgggcg tgaatatctg ttacatctgc agcagaaaga gtgctatcaa tagtgcctcc
     8881 tggtattaca gattttattt taatctcata aatatattca ctgatttcag ggatgtgtct
     8941 tgagtacgct tttccgtcag ccccgaccgt cgtgacaagt ccctcaccat ctgcactaaa
     9001 cagaatttga tttgttttgg tgtttacaaa ataagcaacc tcatttgcat agtcgtaata
     9061 agatgctatt tcactacgcg gtgctaataa ttctgtattc gtcaaaccag atttaccaac
     9121 atcgtaaaga gaatacttta aatatttccc tgccgtgttt tgtatattta cactttcagg
     9181 ttttctcgtg tctgcattta ccacaactcc atccagatac ccttttcctg agaagccgag
     9241 gtctctccaa aaatcaattg gagttttata ttcatgtcct ttcaactgat caacagtcac
     9301 tccataatat gaagtgccat taattccaga ggcgtctgct gcatatacgc cagcagaaaa
     9361 taccgacagg cttaatgctg gcattaaagc caacaatagc ttgcttgcct ttttcatgat
     9421 gtgtaccctt atgttaaatc atgttataaa tatgttgaca ttcggaacgc aaatgtcttc
     9481 agggatacta ctcacaataa caaggcaaat aaaatattaa aatcaatgag ttaagaaaaa
     9541 ttaataatta aaatcacaag aatgcaatac gcatcaaaaa cttaccctaa ataacaccag
     9601 tattactcag aaaaaacaaa acattttctg tggttattat ttaccattga taaatattac
     9661 ctctctctgt cagcctgcat tgtataaaac atacactaca aacgggtgcc atatcggctg
     9721 aacctcagca accagttgac ggggactttc gtccccgtcg cggttttcct actgcttaca
     9781 ctgtaagaac gccgcaaact ccgctcccca caaattcagc cggaactcac acagcgaacc
     9841 gtacaacatc cagatagtga agataaccgt catacaaatt gtgacggtga taagcgattt
     9901 ttgcgacata gcgcttgact ccttttacag agaggcgcta accttctact tgcttaaggt
     9961 atgatgatta gggcctcggg ttaacgttaa gttgactcgg ggcctttcca catcaggcct
    10021 tcaggttctc cctccagcca tcagccgaaa ggcacccacg cataatttac ggtttttgcc
    10081 ctgtacgggc aataaaaaac ccgccatcac agcgggtagt aaacggttac cggatacagg
    10141 tcagaatttc aggccaatgc cagcagtaac gccactcgtg cgccagtcgc cagagccgga
    10201 accttcgtaa gccacatcca gtgtaacact ctcattcagg ttaaactgtg cacctgcagc
    10261 ccacgcaagg gaggtttttt tcgtgctgtt gctttcagaa aatccgccac tactgttaat
    10321 attgtcctta attttcaggt cagcgctaac tttagcaacg cccatccctg ccatcgcata
    10381 taaactcata tactgattaa cccgccatga aggccccgct aacagactcc agtaattcgc
    10441 cctgatatcc gttctggcag aagctgccgg attcttaatc ttcctggtat gatcggctga
    10501 ctgcacatca ataaatgtct gtgagttagt gagagaacgc gtccatgtaa aagaggtgat
    10561 aacgccaaaa tcatccgtta tctcgtagcg atacctgata ttaatgccct gtggattttt
    10621 atccttgcct tcatatccac tgacccgacc atctgtatat tcgcccaatg aagaaaagta
    10681 gtttctgttg acgaaatgac tgaaagtctc cctgttatga gcagtcgcat cctttacaaa
    10741 atccttcagt cccggaaact gaaagtgcgc ataccccaga gaaattgtat ggtttccttc
    10801 tgctgcatgt gcagataaat ttacacaggc aatcccggag attgcacaca caaccagtgt
    10861 tgctatactc ttcatttaag atatcctgta ttcagataac acgctaaact ttttaataat
    10921 acatcttttt agacatttta aatgaatatt aaagactcgc ttcaactaat catatactga
    10981 tgtattaata accaggacct tatctatata cggctttcga atatcccatg ttgcaccacc
    11041 ggaataatgc tcacaggaat atattttatt tcctgtagca ccagtggatg tggtataaat
    11101 cggtcgatca taaggagttc ttttccagtt ataatacccg accagtgctg gcatgattgc
    11161 gcatggatat ccaaggtcct tttcaaattt gatatcaaca ggtattaact tcgcgtcaag
    11221 cagcatcatt tcgccatgat aaatcatctc accgtccggg ttatacatgg cgataccata
    11281 ctcagaaggt ggagtaacca tattcgcgaa agcataaacc gtcgtaacac cagggttcgt
    11341 ttccctgaca attacatgaa gccttagtgc atgatatcca tcaatctgtt catgcgtgta
    11401 catgacatcg gccttcttct ctgttctgat aaagaaaaaa caacttttgc ctgacgggat
    11461 tgatgtttta aaaaaagact tttcagtcgc cggtatggtg cctttgttga tcagacactg
    11521 cggcgtaaaa cctggactta tccatacgct gccatccggc ttcataatgc tcaaaccata
    11581 catgacactt atccccagaa tgtataaata taagatccca taccctgctc aagattcgac
    11641 cacgtcaccg tattgccatt aatggttatc ttcggtactt tccgatccgc aaatacatta
    11701 ttccagggaa ataaacaaca tacagcctgc aatgatttcc cgtcgggttt attcgtgtac
    11761 gtctttgagc cagactccgc tgtaaatctg tccaggaaaa ataccggagt cagcacgccc
    11821 gtaacattaa cgttatttct gttataaatg gcaaaaccgt attccaatac tcacctcctg
    11881 atcagcgtaa tctgcctatg cgaacagcca gtcgtccatt ctgatcataa acctcaattt
    11941 tatcattgcg gatcaccagt cctacattct gattagagta acgaattgtc agttgccctt
    12001 gtgacgtaac actgaaaagg cctccaatat tcaggttacc ctgagaatca acctgaaagt
    12061 ttccgttctg aataacggca ctccggataa ctggcgaagt gatacttacc ccggctttta
    12121 cctcatccgc cacaaccttc cgcgacacca gtgtttcaat caccgcgtca taaatcatcg
    12181 ctttcgggat cacaaccttg ccacctgata ccgcaaacgg ataggcggtg ttatccgggt
    12241 tgttcgggtc aaagacaaac aactgcgacg cagaaattgc aacctgactt acaggcctgc
    12301 cttcactgtc ttttccggcg acaatcccga tccccgcagt gataccatca actcccgctt
    12361 tttttgacca cattgccaga aacgcctcac cgccttcttt atccagttta gtgatgcgct
    12421 tgtcgacctc attaagcgat tcactggttg acgaatccag tgtgctaatg cgggtttcaa
    12481 taccaccaat cgttcttgtc gtttcttccc tgagagtccc cacaacttcg gttgtcttaa
    12541 ttgcagcatc ctttacagcc tgcccctgcg cgttttttat ttctttacgc agctcggaca
    12601 caaccggcga ctttgcagcc tcatcgcgga tctggtcaat gatggccttc acgccgatct
    12661 gtgtttgtgc ctgagtgcct ttttcagcat tccatggacc tttcactcct gccgcgttaa
    12721 caaaacgtat ccagtaaaat cccgaccagc cagggtcaac cggatcgccg taaacctgcc
    12781 ccggcgtcgt ggcaaccagc actgcatcag caaggtcatc ctccgtaccc cgccagattt
    12841 cagtcagtga atgtccgcga taattaggca tatcccattc aagaagaacc gagccaaatc
    12901 ctcctgtcgc cttaaaattc agcggttttg tgggaaaatc aacagtcatt aaagtactgt
    12961 caatctcaat acccggattc agtgcatatg aggcaccacc cgatgttcga cgccgggcga
    13021 gtttaagacc aaccagttcc tcacgggtca caaatgcgtg gcgtccgtca ccacgctgcc
    13081 cggtgccaat ttccatgttc tccacaactg tggataaatc cttccccgca cgccacggtt
    13141 ttctggtcat accggcatct ccgacatcga tgtactcagg gttattcgtt ccacctgccc
    13201 gaatccggat accatcacct gccagttttg cccggttgct gccggaagtc tcaccacact
    13261 tcccttaaac gtacccggcg caaaatgaat cacaggaaca tcatcagcca taatggtgat
    13321 ccccacccgc tcaggcgccg gagatttcac cctgatacag gaaaaagagg ttctttcagg
    13381 taatgaaaaa atttttgaat gccaccttat cgtggagggc agagagcccc cggcaagcac
    13441 tgacattttg tctcctgtca ccacacgcat catatctttc gc
//
LOCUS       DAZUCC010000138        13202 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZUCC010000138 DAZUCC010000000
VERSION     DAZUCC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.139"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            208..1305
                     /gene="mltA"
                     /locus_tag="ACHY09_003423"
     CDS             208..1305
                     /gene="mltA"
                     /locus_tag="ACHY09_003423"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFS3886352.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1382..2188
                     /gene="tcdA"
                     /locus_tag="ACHY09_003424"
     CDS             1382..2188
                     /gene="tcdA"
                     /locus_tag="ACHY09_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFS3886353.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2239..2682)
                     /gene="csdE"
                     /locus_tag="ACHY09_003425"
     CDS             complement(2239..2682)
                     /gene="csdE"
                     /locus_tag="ACHY09_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFS3886354.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2682..3887)
                     /gene="csdA"
                     /locus_tag="ACHY09_003426"
     CDS             complement(2682..3887)
                     /gene="csdA"
                     /locus_tag="ACHY09_003426"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFS3886355.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATTLKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPHLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4079..4306
                     /gene="ygdI"
                     /locus_tag="ACHY09_003427"
     CDS             4079..4306
                     /gene="ygdI"
                     /locus_tag="ACHY09_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFS3886356.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4657..5574
                     /gene="gcvA"
                     /locus_tag="ACHY09_003428"
     CDS             4657..5574
                     /gene="gcvA"
                     /locus_tag="ACHY09_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFS3886357.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5593..5988
                     /gene="ygdD"
                     /locus_tag="ACHY09_003429"
     CDS             5593..5988
                     /gene="ygdD"
                     /locus_tag="ACHY09_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFS3886358.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5981..7081
                     /gene="rlmM"
                     /locus_tag="ACHY09_003430"
     CDS             5981..7081
                     /gene="rlmM"
                     /locus_tag="ACHY09_003430"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFS3886359.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7125..7856)
                     /gene="fucR"
                     /locus_tag="ACHY09_003431"
     CDS             complement(7125..7856)
                     /gene="fucR"
                     /locus_tag="ACHY09_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFS3886360.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVAASLVTA"
     gene            complement(7914..8336)
                     /gene="fucU"
                     /locus_tag="ACHY09_003432"
     CDS             complement(7914..8336)
                     /gene="fucU"
                     /locus_tag="ACHY09_003432"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFS3886361.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8338..9756)
                     /gene="fucK"
                     /locus_tag="ACHY09_003433"
     CDS             complement(8338..9756)
                     /gene="fucK"
                     /locus_tag="ACHY09_003433"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFS3886362.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9865..11640)
                     /gene="fucI"
                     /locus_tag="ACHY09_003434"
     CDS             complement(9865..11640)
                     /gene="fucI"
                     /locus_tag="ACHY09_003434"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFS3886363.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQIFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11673..12989)
                     /gene="fucP"
                     /locus_tag="ACHY09_003435"
     CDS             complement(11673..12989)
                     /gene="fucP"
                     /locus_tag="ACHY09_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFS3886364.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3187 a         3271 c         3474 g         3270 t
ORIGIN      
        1 ccgtggatgc gcactatact ctgctcgcgt tttcatgcaa cctttttttt accccgatca
       61 cggattcggc atgattttga acaaaaacaa atcactaaag cctatttttt gtgcaaaatt
      121 tgcgtcaggg agaggctgct catttcatta aacgcggtgg tttgttatct tcgttgcgcc
      181 ttatttttta acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg
      241 ggcacggttg tggcaatgct tgccgcctgc tcttccaaac caaccgatcg cggacagcaa
      301 tataaagacg ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc
      361 gcgccgatta atgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg
      421 cgtctgtatg gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc
      481 ggtgataccc gcaatatgcg ccagttcggc attgatgcct ggcagatgga aggtgtcgac
      541 aactatggta acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc
      601 cgtcagggcg agttccagta tccgatttat cgtatgccgc caaaacgtgg tcgtctgccg
      661 tctcgtgcgg agatctacgc aggagcgctg agtgataaat atattctcgc ttacagtaac
      721 tccctgatgg ataacttcat tatggatgtg cagggtagcg ggtatatcga ctttggtgat
      781 ggcagtccgc ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt
      841 ggtaaggtgt tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt
      901 cgtcactggg gcgaaacaca cagtgaagcg gaggttcgcg agctgctgga acagaacccg
      961 tctttcgtct tctttaaacc gcaatctttt gcaccggtga aaggggcaag tgcggtgccg
     1021 ctggttggcc gtgcttctgt tgcctccgat cgttcaatta ttccgccagg tactaccttg
     1081 ctggcagaag tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt
     1141 ctgatggtgg cgctggatgt cggtggcgca atcaaaggcc aacacttcga tatctatcaa
     1201 gggatcgggc cggaagccgg acaccgcgca ggttggtaca accactatgg acgtgtctgg
     1261 gtgctgaaaa ccgccccggg cgcaggtaac gtctttagcg gctgatgtga tattctgtat
     1321 gccgttttta cggatatgac agggtgaggg gtaacctcac cctttttaat tctgaggtgc
     1381 tatgtctgtg gtaattagtg atgcatggcg tcagcgtttt ggtggcacag cgcgtctgta
     1441 tggtgaaaaa gcattgcaac tgtttgctga cgcgcatatt tgtgtggttg gtatcggtgg
     1501 tgtcggttcc tgggcggcgg aagcgctagc gcgcacgggg attggcgcaa tcacgcttat
     1561 cgatatggat gatgtgtgcg tcaccaatac caatcggcaa attcacgctc tgcgcgataa
     1621 cgtcgggctg gcaaaagcgg aagtaatggc ggagcgtatt cgccagatta acccggagtg
     1681 ccgtgtaaca gtagtggatg acttcgtgac gccggataac gtagcgcaat atatgaatgc
     1741 cggttattcg tacgtgattg atgctattga tagtgtacgg cccaaagcgg cgctgattgc
     1801 ttattgtcgg cgcaataaaa tcccgctggt cacgaccggt ggcgctggtg ggcagattga
     1861 cccgacgcag attcaggtta ccgatctggc gaaaacgatt caggacccgt tggcggcgaa
     1921 gttgcgcgag cgcctgaaaa gcgattttgg cgtagtgaaa aacagtaaag gtaagctcgg
     1981 cgtggattgc gtgttctcta ccgaagcgct ggtgtacccg cagtcagacg gcaccgtctg
     2041 tgcgatgaaa gccacggcgg aagggccaaa gcggatggat tgtgcctcag gatttggcgc
     2101 ggcgacgatg gtgaccgcca cttttggctt tgttgcggtt tctcatgcgc tgaagaagat
     2161 gatggcgaaa gcggcgcgtc aggggtaaac aatcaggtcg gatgcgacgc gaacgtctta
     2221 tccgacctgg ttctgtgctt aaacctgctt cgccgcagcg ataatcgcct cgcttaacgc
     2281 attcaacccc tggctgcgtg aggcgctaag ttgcgcacgt aatcccagct catcaaacaa
     2341 tgccagcggc gactgtgcct gcaattcggc ggcggttttc ccctcaacgg cagtcaacaa
     2401 caccgccaac aggccgcgca caatgcgccc ttcgctgtcg ccaaagaaat gcatcttgcc
     2461 gttttcagcc actgtatatc ccagccagac gcggttttcg catccggcaa tctctttagc
     2521 ctgcgctttt aactcctctg gcaaagccgg aagctgtttc cccagcatga tcaactggcg
     2581 atatttatct tcccattgcg tcaacggtgc gaaggtattg cgtaacgttt ctgcggttac
     2641 ggttgtgccg aacggatgtc cggcgaattg cgggtttgtc attaatccac caataattcc
     2701 agcgcgcggt caacggcatt caccagcgca tccacatcac tctttgtatt atatggcgca
     2761 aatgaggcgc gcagtgtgcc ggttacgcct aattctgcca gtagcggctg agcgcaatgc
     2821 tgcccggccc gcagggcaat accgtactcc gccagcagcg tcaccatatc gctgtgatga
     2881 acgccagcaa aatcaaaggc cagcaggctg gaatcctggc agcgaaatga gcggaagcca
     2941 ggacgttttg ccagcgcatc ttccgccagc gttgctaagc tacggctcca gctttcggcc
     3001 tggttgatat cgtaatctgc cagccattcc agcgccgcgc ttaatcctat gacaccagcg
     3061 acatttggcg ttccagcttc cagtttccac ggcgcagatt gagtcgtgaa gccgtcaaaa
     3121 ctcacttcgt gaaccatttt gccgccgccc agccagggcg acatcgcttc cagcagttct
     3181 gatttgccat acagcacgcc gatacctgtc gggccataca gtttgtgacc tgaaaaagca
     3241 tagaaatcaa tatccagttg ctgaacatcc gcggggaaat gcaccgcccc ctgagcacca
     3301 tcaaccatca ccaccatccc ggctgaatga gcaaaggtaa tcgctcgcgc cagatccggg
     3361 caaccgccag tgacgttcga catttgtccc agagcaagaa ttcgactacg aggtgtgatc
     3421 agttctggca acaaatcgac atccggcagt cgctgcgcat taagcggcaa tttcaccact
     3481 ttggctccag tttgttgggc gaccatcagc caggggacga ggttggcgtg gtgttctgcc
     3541 acgctgacaa taatctcatc gcccggttgc agatgcggac gcgcatagca ttgcgccacc
     3601 atgttgatgg attcagtggt gccgcgcgtc cagacgatag ttttgtcatc tggtgcattc
     3661 agtaattgcg ccactttctc ccgtgcagct tcataacgcg cggtcaggcg ttgggcttcg
     3721 gcaaactggc tgcgatggac gtttccggcg ctcaggctgt aaaactgtcg ggtggcttca
     3781 accacggctt caggtttaag cgtggtcgcg gcgctgtcga gatagacgcc cgcatcctgt
     3841 agcgcgggaa actgggcgcg aaactgcgcg ggattaaaaa cgttcatggt actcctcggc
     3901 ttgatgcacc gatcgtgacg caaaattcac tttgatacaa ggagagtgct aaccatcaga
     3961 attatctgga tatcctggcg aagccgcgtc agtaacatat gctgaatagt gttaggcgtt
     4021 aattttagcc tgttaatcaa tactattaag atttattaat cgctttaagg agaagatgat
     4081 gaaaaagact gccgcaatta tttctgcctg tatgctgact tttgccctga gcgcctgttc
     4141 cggttcgaac tatgtgatgc acaccaatga cggacgtacc atcgtctctg acggcaaacc
     4201 acagactgat aacgataccg gtatgatttc gtataaagac gctaatggca acaaacagca
     4261 gatcaaccgt accgacgtga aagagatggt cgaactggat cagtaattcg cgatcgcaag
     4321 gtaaaaaaaa gcaccgcaat taggcggtgc tacattaatc actatggaca gacagggtaa
     4381 atgtacagga agtgaaaaaa ggtagctttg ctaccatggt ctgaatcgca gaccaattgc
     4441 aaacacaaca acacaacatc acaaccgtaa gccaaaagtt caccagaaca cgcattccga
     4501 taaaactttt cgttccggct caggaagtgc cgccactata ggtatttgct ggtagaagct
     4561 caacggacaa tttataatgg ctcagattaa aaaaactaat aggttacata gtgtaatcta
     4621 attgttaaat tcatttaaca tcaaagttta aaagccatgt ctaaacgatt accaccgcta
     4681 aatgccttac gagtttttga tgccgcagca cgccatttaa gtttcactcg cgcagcagaa
     4741 gagctttttg tgacccaggc cgcagtaagt catcaaatca agtctcttga ggattttctg
     4801 gggctaaaac tgttccgccg ccgtaatcgt tcactcctgc tgaccgagga agggcaaagc
     4861 tatttcctcg atatcaaaga gatattttcg caattaaccg aagcgacgcg taaactccag
     4921 gcccgtagcg ctaagggggc gttgacggtc agtttactcc ccagtttcgc cattcattgg
     4981 ttggttccgc gactttccag ctttaattca gcttatccgg gaattgacgt tcgaatccag
     5041 gcggttgatc gtcaggaaga taagctggcg gatgatgttg atgtggcgat attttatggt
     5101 cggggcaact ggccggggct acgggtggaa aaactttacg ccgaatattt attgccggtg
     5161 tgttcgccgc tactgctgac tggcgaaaaa cccttgaaga ccccggaaga tctggctaaa
     5221 catacgttat tacatgatgc gtcgcgtcgt gactggcaga catatacccg acagttgggg
     5281 ttaaatcata tcaacgttca gcaagggcca atttttagcc atagcgccat ggtgctgcaa
     5341 gcggctatcc acgggcaggg agtggcgctg gcaaataacg tgatggcgca atctgaaatt
     5401 gaggccggac gtcttgtttg cccgtttaat gatgttctgg tcagtaaaaa cgctttttat
     5461 ctggtttgtc atgacagcca ggcagaactg ggtaaaatag ccgcctttcg ccaatggatc
     5521 ctggcgaaag ccgctgctga acaagaaaaa ttccgctttc gttatgaaca ataatttacg
     5581 tagggtacga ccatgaccag ccgttttatg ctgattttcg ccgccattag cggcttcatt
     5641 tttgtagctc tgggcgcttt tggcgcgcat gtgttaagta aaaccatggg ggccgttgag
     5701 atgggctgga tccagaccgg cctcgaatac caggcgtttc atacgctggc gatcttaggt
     5761 ctggcggtgg caatgcagcg tcgcatcagt atctggtttt actggagtag cgttttcctc
     5821 gcgttaggca cggtgctgtt cagcggcagc ctttattgcc tggcgctgtc ccatctgcgt
     5881 ttgtgggcgt ttgtcacacc ggttggcggc gtgagcttcc ttgcgggctg ggcgttaatg
     5941 ttagttggtg ctatccgttt aaagcgcaag ggcgtaagtc atgaataagg ttgtattgct
     6001 gtgccgtccg ggctttgaaa aagagtgcgc cgcagaaatt accgataaag ccggccagcg
     6061 ggaaattttc ggttttgccc gcgtgaaaga gaatgcgggt tatgtcattt atgaatgtta
     6121 tcaacctgat gatggcgata agttaatccg tgagctgccg ttcagttcat taatttttgc
     6181 ccgccagtgg tttgtggtgg gggaactcct gcagcatttg ccgccagaag atcgtattac
     6241 ccccattgtc ggcatgttac agggtgtagt agagaagggc ggtgaactgc gtgttgaagt
     6301 tgccgatacc aacgaaagca aagagttgct gaaattctgc cgtaaattca ccgtgccgct
     6361 acgcgctgcc ttgcgcgatg cgggtgtgct ggcgaactat gaaacgccga agcgtccggt
     6421 tgtgcatgta ttctttattg caccaggctg ctgctatacc ggttactcat acagcaacaa
     6481 taattcgccg ttctatatgg gcattccgcg cctgaaattt ccggcagatg cgccgagtcg
     6541 ttccacgctc aaactggaag aggcatttca tgtgtttatt cctgcggatg agtgggatga
     6601 acgcctggcg aacgggatgt gggcggtgga tttaggcgct tgcccaggcg gctggaccta
     6661 ccaactggtg aagcgcaaca tgtgggttta ttccgtcgac aacggcccga tggcgcaaag
     6721 tctgatggat accggacagg tgacgtggct gcgggaagac ggtttcaaat tccgtccgac
     6781 acgcagcaat atctcctgga tggtatgcga tatggttgaa aaaccggcga aagttgcggc
     6841 attgatggcg cagtggctgg ttaatggctg gtgccgtgaa accattttca acctcaaact
     6901 gccgatgaaa aaacgctacg aagaagtgtc acacaatctg gcgtatattc aggcacagct
     6961 tgatgaacat ggcataaatg ctcagattca ggcgcggcag ttgtatcacg atcgcgaaga
     7021 agtgacggtg cacgtccgcc gtatctgggc tgcggtgggt ggtcgtcgcg acgagcgata
     7081 acaggcaagg ccggggcgtc gtttccggcc tgctatcctt tccgtcaggc tgttaccaaa
     7141 gaagctgcaa cctggcgctc atcagaaata atgtgcgtga cctcatccag atgcccgatg
     7201 cgggcttccc ctgaacgatt aaatttactt ttatcaatca ataacaacga ttgcgcggca
     7261 cgttttaata gcatcgattt gtaatcagcg ttgatggcat tggagtccca cagtgcgccg
     7321 ctgctatcga tcccttcaca agaaaaaata aacagatcga tttccagcga tttaagctgg
     7381 gaaatcagcg agggattgac gtaacaacca tatttgcgct caagtgtgcc gccggaactg
     7441 atcagttgaa tgcgttcgcg tttaccgagt tcatggcaaa tcggatggct attggtgaag
     7501 acctggatgt tgatgtcagg caactggcgt gccagatacc agcaggttga acttgcgtct
     7561 aaggctatta ccatcccttc ttcaatccac gcgagcgcct cgcgcgcgat atctgcttta
     7621 tgcgcataat ggcttttcag ccggatgtga aagggatcgc cactgtcttg attttgacgg
     7681 tggatatatt tagcgcgccc atgattgcgc agaattttac cctgcgtctg taattcattg
     7741 agatcgcgac gaatggtttc tttactgacc tttagctgtt cagagagagc ttccgtggtc
     7801 aggctggtat ggttcagcag caggtcgact atcgcttgct ggcgtgccgc tttcatattt
     7861 ttcaccccat cattcgataa ccgcccgcgt catgcgggcg taccggtatg agattacggt
     7921 gttacccctt tttttaaaag aatattcccg tacttcgctc gttcgcctgt gataacgatc
     7981 gcaaaggctt tttgtgcccg ttcataaaac gcaaaacgat tgatgcggat gatgtcagga
     8041 cagggggctt gtagtgaaag cgcattacga taacgtcgtt ctacttcagg atctagagtg
     8101 tcaccttcta ccgccgccat catcaccagc ggtggtgcat aactgtccag ctcaaataac
     8161 gggataatcg cctggagcaa gtcgctcacc aacaggccat cagcgcggat cacctgcggt
     8221 cccatcgaat gggcgggaaa gtgagcatcg gaaaaaataa tttcatctcc atgccccatc
     8281 tctgccagca ctttcaatag ttcgggagaa attaacggcg aaattgtttt cagcatttca
     8341 cacctcctct ataaattcag gttcagtttg cgggtagaaa taacggtact gataatgaat
     8401 ctgcgcgcgg gcttcttccg gactgttaaa ttcccctacg ccataccaac caaacagcgc
     8461 agctcctgcg acggtcgttt cggcatcgtc gagaactttt accggaatat caagcatatt
     8521 ggctttaatc tgattccaca atgtgttgcg acttcctccg ccgactaaca ataattcaga
     8581 ggctttaaag tgcccgattt tttccagcat ctgtagattg cgctgtaatt gcgtggttaa
     8641 cccttccagc gccgcgcgat agaaatgccc ccgcgtggta ttaagcgtta cgccttgcca
     8701 gccagcgttc tggcacgaca ataaatcaca ctgcattttc actccatctg cgccaggcgc
     8761 gatcaggcga gcttcttcaa tcaatatttg ccagggagtt tcagctgtcc agaacagttt
     8821 tctcacccat tccagcacgc cggatgccag ccattgcata cctgggttat acaaccctgc
     8881 ctggctatcc agttcgcaag tagaaccggc gtactgactt aacagcgaag tatcaacctg
     8941 ggcgctgcgg accattaaaa tttcccatgt accggaagag agcacgggtt cattttgttc
     9001 ggcaccagcg ccaaaaaggg cgaactgggt atcgtgacct gcggaaatca ccggtatgcc
     9061 aacgggtaag ccgagcattg ctgcggcgct gttctgtagc gtaccaatct gttcacccgc
     9121 ttccaccaga cgagggaaga gtcggcgtgg aataccggtg gcttgtaaaa tttgcggact
     9181 gaaatcgcgt tgctggatat ccagcatctg gctggttccg gccatcgtaa tatcagtagt
     9241 gaattcgccg gttaaacggt ggttaatcag tgacgaaata aagagccagg cgtgcgcgcg
     9301 ttccagcagt tgtggatgat tttctttcaa ccacaccaac ttatataacg tattgaaact
     9361 aaaggctccg actccagaaa tagcctgcaa ctgctgtgcg gagatcaacc gttcaatatt
     9421 gtccattacc gcggctgttc gcggacattt ccagctaatg atcggataga gcagattgcc
     9481 ttgcttatct accagagcgc catccacacc aaaggtggtg acggcgatac cgcggatatg
     9541 gcaatcagtc agttcactat tgatttgccg acagcaatca gcaaagcgtt gcaaaatggc
     9601 gtctaaagac cactggtgcc aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt
     9661 tgaggcgcgg gcaacaattt ttccctgccg attaaccgcg atggccctga cattggtcgc
     9721 gccacagtcg agtaccagga taacttcttg tttcataatc gctcctgcaa tatagccgga
     9781 taacattgct tatccggcta accactcttg cttgttcagg cccggtagcc ggagcgaccg
     9841 ggcatcacat cagggagtca tgtattaacg cttgtacaac gggccgtagt tctggcaagc
     9901 gcggtaatcc tggccttcaa tatccatgcc gtgcgcggcc caggcagaag gacgatacac
     9961 tttggtctct tcaacgttgt gcatacatac cgggatacgc agcatggagg caagagtgat
    10021 aaagtctgcg ccaacgtggc cgatggtcag aactccgtgg ttagcgcccc agttcgccat
    10081 taccgagtac acatccgtaa acggcccttt accggtgagg cgcggtgcaa accaggtggt
    10141 tggccaggtt gagttggtgc gtttgttgag gatgtcatgc acatccttcg gcaattccac
    10201 gctccagcct tccgcgattt gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt
    10261 catggtgaac gggacgccgc cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa
    10321 gtattcgtgg atcgccgggc accattcggt agcggcgagg caagcgtcag cctcttgctg
    10381 agagatttcc cagtgcggct tcatcgtcgg tttaccttcg ctgtcgcgtt gtttacagga
    10441 gccgtccagc gcagcagaac cggagttgat caaatggatg atgccgtgtt ctgccagccc
    10501 gtccagttta tgccccgtta cacgctcaat tgcttctggt gaccagtagg tacgcacatc
    10561 ggcaaatatc tgagcggtgc cggtgagctg gtggcccatt aacattgcca cgccgttaag
    10621 actgtcgttt tcggtcgcca cgacaaacgg tttgcgcacg ccattccagt caaatgaact
    10681 gttgaggatc gcttcagcgg tatcgccatt gggatattga tcggtccagt gacgttgccc
    10741 ctggaagcct gcagcgatgg cgttgtagcc aagtgattct tccacgcggc caatatcggc
    10801 cagtttgctg ttgccttgca tcatgtcgcg gatacacatc gccatcagta aactttcgcg
    10861 cagaactgcg cggctttgct cggcattgcg ttgatactgt ttgttatttt catcttcgcc
    10921 atagcggaag tttttatcag cccaggccag cgccatttcc agttcggctt cgtcgtaaat
    10981 cttctgatcg atacggcgac gcagttcggt catatccacc gcctggactt tcattcccag
    11041 ccaggattca aagaagttgt gatcaacgat ggaaccggcg atacccatcg aaacgccgcc
    11101 cagcgacaga tagcttttac ctttcatgct ggcgactgct aaaccggcgc gggcaaagcg
    11161 cagcagtttt tcttcaacat cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg
    11221 accgtaaatg gagaatgccg ggatgccttt ctggctgtga gctgccagag ccgctgccag
    11281 gtaaacagcg ccgggacgtt cagtgccgtt aaagccccaa atggctttcg ggcgggttgg
    11341 atccatatcg atggtttcac tgccatagca ccagcaaggg gttaccgtaa tggtgaggcc
    11401 tacattctga ctgctgaatt tttcttcgca agcagcggct tcagccatac ccgcgataca
    11461 ggtatcggaa atgacacact cgacggcagc tccacaggca tggcgcagtt tctcggtcag
    11521 cagtgcagcc gtggctttcg ccatattcat tgtttgttct tcaagcgact cacgaacacc
    11581 catgcgacga ccgtcaataa ccgggcggat accaattttc ggtaagctga tttttttcat
    11641 tacagattcc tcactttatt cggaaaatat gttcagttag ttgctgtttg agaacggaaa
    11701 cgggcaaaga taaagatgac cgcgaagcag agtgcgggga tcagttcagc agtggggatg
    11761 ttgcccgccg cgtcactgac aaaacccatg accggagtga caataccgcc gccaatgatg
    11821 gtcataacga tgaaggacga accgtatttg gtgtcctggc cgagattctt aatgcccagc
    11881 gagaagattg ttgggtactg aatcgacata aaggcgctgc ataaagtcag ggctattaag
    11941 cccacatgac cgccagcgaa ggctgagatc aggcacagtg ccatagcgat taatgcgtag
    12001 gctgccagga ctttgtgtgg tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca
    12061 ataaagaagc acaccatggt tccggttaaa tagttagcgg caaagcctgc ggtcatacct
    12121 ggaatttctt ctacagcgta gcgaatcaaa tagctccagc aggccgtttg cgcgccgaca
    12181 tagcagaatt gcgctaatac cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc
    12241 gatgcggaga acgatccttg tttggcgtca ctgtgattat cactctgcaa tgccgggaat
    12301 ttcgtcagca tgatcagcag ggcgaccagt aacacgatag ccacgatgat catataaggt
    12361 gtctgtaccg ataataccag gctgtgttta tacgcactca attgctctgg agacatttta
    12421 tcgagaacgt cttgcgattg atgtggcacg ttagacaaaa taagactttg tccaaagaca
    12481 accgcgataa ttgcgccaaa cgagttaaat gtttgcgcaa gatttaagcg gaagtgacca
    12541 ctgctttccg gccctaatac cgtaacaaaa gggtttgcgg cagtttccag acaacctaat
    12601 ccagctgcaa taataaatag gccaactaaa aataaggtgt agttcattat ttctgcagcg
    12661 ggccagaata atgcagcgcc caaggcatat aaaaataatc cggtaataat ccctgcttta
    12721 taactgagtt ttttcatcaa tatcccagca gggattggga taatgaaata accaaagtaa
    12781 aaggccgatt ggatcaggcc agcctggaaa tttgtcagcg taaaagcctg ctggaattga
    12841 ggtaataaaa tgtcgttaag gttattggct accgcccaaa gaaaaaacag tgagcacagc
    12901 agcgcgaatg gaataatgta acttctgctt tgccctgcat ctttatctac cgcacggtaa
    12961 ctctgcgttt gtattgatgt gtttcccata gcatcctctt aggttcagaa gcttaagcac
    13021 gtaatcggtt atgttttcaa aagtgcaaaa ccagcgagtt cgggcttcat atcagtgaat
    13081 atttgcctgt cagaaatgaa ctcatggatt cattttcaaa taaaaaacgc tctgtaatat
    13141 gacggcggtc acacttatta gttgggaggt gactttatgt gactaccatc actttaataa
    13201 tc
//
LOCUS       DAZUCC010000139        13181 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZUCC010000139 DAZUCC010000000
VERSION     DAZUCC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.140"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(227..373)
                     /gene="ygdT"
                     /locus_tag="ACHY09_003436"
     CDS             complement(227..373)
                     /gene="ygdT"
                     /locus_tag="ACHY09_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFS3886365.1"
                     /translation="MLSTESWDNCEKPSLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            559..1248
                     /gene="mutH"
                     /locus_tag="ACHY09_003437"
     CDS             559..1248
                     /gene="mutH"
                     /locus_tag="ACHY09_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFS3886366.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1317..2030
                     /locus_tag="ACHY09_003438"
     CDS             1317..2030
                     /locus_tag="ACHY09_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFS3886367.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2168..2386
                     /gene="ygdR"
                     /locus_tag="ACHY09_003439"
     CDS             2168..2386
                     /gene="ygdR"
                     /locus_tag="ACHY09_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFS3886368.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2494..3534
                     /locus_tag="ACHY09_003440"
     CDS             2494..3534
                     /locus_tag="ACHY09_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFS3886369.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3566..4759)
                     /gene="lplT"
                     /locus_tag="ACHY09_003441"
     CDS             complement(3566..4759)
                     /gene="lplT"
                     /locus_tag="ACHY09_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFS3886370.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLSAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRY"
     gene            complement(4752..6911)
                     /gene="aas"
                     /locus_tag="ACHY09_003442"
     CDS             complement(4752..6911)
                     /gene="aas"
                     /locus_tag="ACHY09_003442"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFS3886371.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7497..8528
                     /gene="galR"
                     /locus_tag="ACHY09_003443"
     CDS             7497..8528
                     /gene="galR"
                     /locus_tag="ACHY09_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFS3886372.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8535..9797)
                     /gene="lysA"
                     /locus_tag="ACHY09_003444"
     CDS             complement(8535..9797)
                     /gene="lysA"
                     /locus_tag="ACHY09_003444"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS3886373.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAARQQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9919..10854
                     /gene="lysR"
                     /locus_tag="ACHY09_003445"
     CDS             9919..10854
                     /gene="lysR"
                     /locus_tag="ACHY09_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFS3886374.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10841..11533)
                     /gene="ygeA"
                     /locus_tag="ACHY09_003446"
     CDS             complement(10841..11533)
                     /gene="ygeA"
                     /locus_tag="ACHY09_003446"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFS3886375.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAIIRAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADKRAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11662..13080)
                     /gene="araE"
                     /locus_tag="ACHY09_003447"
     CDS             complement(11662..13080)
                     /gene="araE"
                     /locus_tag="ACHY09_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFS3886376.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         3362 a         3531 c         3235 g         3053 t
ORIGIN      
        1 tataaacaga ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt
       61 gaagaactac ggaattacta cgggaaaacc cggagcattc tgaataagac agatacagag
      121 attttaaaat attcattatc agtcaatgca ttacatgtga tttgagtaat aaaaaagagt
      181 aattcgtgac ccaggtcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt
      241 atttatccac agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt
      301 cgcacgtcaa ggctgtaaat ggaaacagta gtgaaggttt ttcacagtta tcccagcttt
      361 ctgtggataa catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacccgt
      421 tgcagtgttg cgcaactcga ttaccggcaa cctaaaaagc aatataaatc agagaattga
      481 acaacgcatg tggaaaaagt tacactgcga atattcggca cttaattgct gtttgttttt
      541 taaccaaggt atcatgacat gtcccaacct cgcccactgc tctctcctcc cgaaactgaa
      601 gaacaattgt tagcgcaagc acaacaactt tctggttata cattgggaga actggcggca
      661 cttgccgggc tggttacgcc ggagaattta aaacgcgata agggctggat tggcgtgtta
      721 ctggagatct ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgctgctctg
      781 ggcgtggaac ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc
      841 gtttgtgttg ccccgttaac gggcaatagc ggggtgacct gggaaaccag ccacgtgcgc
      901 cacaagctca aacgcgtgct gtggataccg gttgaaggcg agcgcagcat cccgctggca
      961 aagcgtcgcg taggatcacc gttgctgtgg agcccgaatg aagaggaaga ccgacagtta
     1021 cgcgaagact gggaagaatt aatggatatg attgttctcg gtcagattga gcggatcacc
     1081 gctcgtcacg gggaatattt acagatacga ccgaaagcag cgaatgcgaa agcgctgacc
     1141 gaagctattg gtgcccgggg cgaacggatt ctgacgctgc cgcgcggatt ttatttgaag
     1201 aagaatttca ccagtgcgct actggcccgt cattttctga tccagtagcc atcgctttga
     1261 cctgccgctt tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt
     1321 tatttgcatg gataaccgat cctaacgcct ggcttgcgct cggtacactg acgctgctgg
     1381 agatcgttct tgggatcgac aatattattt tcctttctct ggtggtggca aagcttccca
     1441 cagcacaacg tgctcatgcg cgccgtctgg ggttggcggg agccatggtc atgcgtctgg
     1501 cgctgctggc atccatcgcc tgggttacgc gcctgacgaa tccgcttttt acaatattca
     1561 gtcaggaaat ttccgcccgt gatttgatcc tgcttctggg tggcttgttc cttatctgga
     1621 aagccagcaa ggaaatccac gaatccatcg aaggtgaaga agaagggctg aaaacacgcg
     1681 tttcatcatt cctcggcgct atcgtgcaga ttatgctgct ggatattatc ttcagcctcg
     1741 actcggtgat taccgctgtg ggtctgtcag atcacctgtt tattatgatg gctgccgtgg
     1801 tgattgccgt aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt gtcgagcgcc
     1861 atccttcggt aaaaatgctg gcgctctctt tcctgattct ggtgggcttt accctgattc
     1921 tggaaagttt cgacatccac gtaccgaaag gttacatcta cttcgcgatg ttcttctcta
     1981 ttgcggttga aagcctcaac ctgattcgca acaaaaagaa tccgctctga tactccgtac
     2041 gctctcctgc gggagggcgt actcactcct gttaccctca cagtttaaga ttttcctgct
     2101 ttcaagatat ataacgtcgg tttataaaca gactattatc ataggtgggc caaatgagga
     2161 taaccggatg aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc
     2221 cgggtgttcc agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg
     2281 aaaacctgaa attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc
     2341 gatgcaaatt aaccgtgatg atgtttcgca aattattgaa cgttaacaaa taaggtcagc
     2401 atccggctgg ccttaagatt tttctctttc ccttttccct tccctctgcc atttttatat
     2461 tccttatgtc gtgattataa aaaggaaacg gctatgcaat atcaccgtat accccacagt
     2521 tcgctggaag tcagcacgct ggggcttggc acgatgacgt ttggtgaaca gaacagcgaa
     2581 gccgacgccc acgcacaact cgactatgcc gtcgctcagg gcattaacct tatcgacgtt
     2641 gccgaaatgt acccagtacc tccgcgcccc gaaactcaag ggctaaccga aacctacgtc
     2701 ggcaactggc tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc caaagtgagc
     2761 ggaccgtcgc gcaataatga caaaggcatc cgcccggatc aggcgctgga tcggaagaat
     2821 atccgcgaag cgctgcatga cagcctcaag cgtctgcaga ctgattacct cgatctttat
     2881 caggtgcact ggccgcagcg cccaaccaac tgcttcggca aactcggtta tagctggaca
     2941 gattctgcgc ctgcggtttc gctgctggat acactggacg cactggcaga gtaccaacgc
     3001 gcgggaaaaa ttcgttatat cggcgtgtcg aacgaaactg catttggcgt aatgcgctac
     3061 ctgcatctgg cggacaaaca cgatctgccg cgtattgtca ccattcagaa cccctacagt
     3121 ctgttaaacc gcagttttga agtaggtctg gcagaagtca gccagtatga aggggtcgaa
     3181 ctgctggcct attcgtgcct gggtttcggc acgctgaccg ggaaatatct caacggtgca
     3241 aaacccgctg gcgcacgtaa tacgctcttt agtcggttca cccgctatag cggtgagcaa
     3301 acgcaaaaag ccgtcgcggc gtatgttgat atagccagac gtcatggcct ggatcctgca
     3361 cagatggcgc tcgcttttgt acgccgtcaa ccgtttgttg ccagcactct gctgggcgca
     3421 accacgatgg agcagctgaa aactaacgtc gaaagtttgc atctggagtt aagcgaagac
     3481 gtattagcag aaattgaagc ggtgcatcag gtttacactt atccggcacc ataagattaa
     3541 cgccggttaa aaccggcgtt aaatattaat aacggcgctg ccagatccac agcgccgtta
     3601 ttgccagcgc aaacagcgca ccgaagccaa tgccaatggg cacgaccggg atgcctacca
     3661 ttaccgccag cgagtaaatg cccagcatca acaacatggc gctgttttcg ccaaggtttt
     3721 gtactgcaat cgcattcccc gccccgacgc tttttttacc ccgttcctgt agcaacgcat
     3781 tgagcggaac gacaaaaaag ccccccagca cgccaatcag catcaacaag gcataggctg
     3841 acagcagctc gtgttgcagg gagaaaatca gtaccaccac gccaatcaaa atcccggctg
     3901 gcatacagcg tgacacggtt tccagcgtaa ctaacttcgc tgccgcacca gcgccaacca
     3961 cgataccaat cgctaccatc gcgttgagat aggtcggcgt agcgttatcg gtaatgccca
     4021 gcgccaccgg tacccacagc accaacagga aacgcagcgt gacacccgct ccccagaata
     4081 aactggtgcc caccagcgaa aaacgcgttt caccattgcg ccatagcgag gtgcaggcat
     4141 tcaggaaact gcgggtcatg ctgatgagat tccaggactg ccccggacgc gccgccgcca
     4201 gtttaggaat gtagatattg gcaacgaccg caccaccgta ggccagtgcg catgcggcca
     4261 gggcgaccag gacatgccag tcagccagta caccaccggc tacggaaccg agcaaaatcg
     4321 ccgctatggt agaagcttcc attaaaccat tagctttcac taacttacta cccgtggtta
     4381 attcgccgag aataccgtat ttcgccggtg aataggctgc agcaccaaca cccaccagcg
     4441 tatagccgag aaacggattg ataccaaagc agatactggc tgcgcccagc agcttcaggc
     4501 cgttggcaaa catcatcacc cggcctttgg cgaagctatc cgccacctgt ccgacaaacg
     4561 gcgcaaaaag aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg
     4621 gataaaactg cgctttcagt aacgccagag tggcaaacag cagcgcatta tcgccaaacg
     4681 cagagagaaa ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag
     4741 tgtgcactga ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca
     4801 aagtcaggtt tgccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt
     4861 acagcaagct ccggcacgcc gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt
     4921 tcgttatctg tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca
     4981 gtggcatgga ctttatctgg cgaaacacca agtgccagtt gttccaccat ttccagcgac
     5041 accatttcgc ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc
     5101 tgctcgtcga aacgcacaat atcgccagtg tcataccagc cgcgctccat ttcgccgcga
     5161 acattctcgg cggtgggcac ttccagtaca cctggcttct ccacccgcag atagccgttc
     5221 attatgttcg gccctttcag ttgcaggcgt ccgccctctt cgataccagg aaccgacaac
     5281 aggcgcgcat ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt
     5341 acgttgatag aaacgacagg cgcgcactcg gtcacaccgt agccttcaag gatgcgcagg
     5401 ccaaatttat cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc
     5461 acatagcgta gacggtagaa gtcatacggg ttggcgaaac gcgcgtagtg accgagaaaa
     5521 gtcgaggtac cgaacaacac ggtgcaactg cggtcataca ccaactccgg cacaatgcgg
     5581 taatgcagcg ggcttggata aaggaacacc tctgcacctg tgagcagtgg cgtaaacagg
     5641 cctaccgtca gcccaaagga gtgaaacagc ggtaacgccg acataaagcg atcgttggtg
     5701 gtgaagtcgg caatcgtttt aatctgctcg acattcgcca gaatgctttt atggctatgg
     5761 acgacgcctt tcggatggcc ttcagaaccg gaggtaaaaa ggatcagcgc ctcttcttcc
     5821 ggctgctgtt ttacctgtgc cagacgcggc atcagcaaat gagcgaagat ccatactttg
     5881 tcggcagtgg tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt
     5941 tgctccggca gatgccagag tttgccttta tcgagaaact gacgggaagt gaaaatggtt
     6001 ttgatttcag ccgccgtaat agcactggtc aaccctttta ccccggcggt gtagttcatc
     6061 attgcgggaa tgcggcgacg ggcgatggcc ccaaaaatca ctgccgcact gatgcctgca
     6121 ttgggcagca ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca
     6181 acaaacagcg ttttcgtaag caatttgcga taggagtccg gggtaaagtt aacgtcttcg
     6241 acacatttct tcccggctcc gaagcggtac attgcgctca gtaaagattc gtacagcgtt
     6301 tcacgcgggc gcaccgccat acgcgcttcc atcattattt gatgcagcat ttcgccagcg
     6361 attttgcgac ggtcacgggc acgcggcgca tccggcatct ccacctgcgt tggtggcaaa
     6421 atatgcagag taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag
     6481 tgcgtaagtt ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg
     6541 acaaatcccg cgccatcgta gattttcatc agcgagccag tggtggtgat gcgtccttca
     6601 gggaaaatca ccactggtcg gccctgttcc accagacgta ccagatgttt aatcgccata
     6661 ggttgcgtcg ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac
     6721 tgttggctta ttgaggtgta aacggcaaat accggacgta cgggtaaaaa caatgccagc
     6781 agtatgccat caataaaaga gacgtgatta ggcgtgatta gaacgcgctc gcccttcagt
     6841 gcctgggtgt cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag
     6901 ctaaaaagca ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac
     6961 acgagataaa gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg
     7021 caggttggtg caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc
     7081 tctgggatct tgattgtggt ctgcacgacg actcttcgcc agtgagaaaa cgcaaaggaa
     7141 tgaagggaaa tgcaacgagg tgtgtaaatt gtcggttact gttacagatt gatgaccggc
     7201 aaaaaaaaac ctgcgcatct gcgcaggctg gtgtaattca tgtgctcaac ccgaagttga
     7261 cttcacctat caatacctct gggatcacca ctttagcaac ctgaagccaa acgccaccag
     7321 cggtcaatcg caacagcgtt tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt
     7381 cttacttatt tgcgtttggc tcacattccc acaatgaaaa cacgccgccc cttgaaccaa
     7441 cgggcgtttt ccgtaacact gaaagaatgt aagcgtttac ccactaaggt attttcatgg
     7501 cgaccataaa ggatgtagcc cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca
     7561 ttaataattc acccaaagcc agcgaagctt cccggcttgc tgtgcatagt gcaatggagt
     7621 ctcttagcta tcacccgaac gccaacgccc gtgcgctggc gcagcagacc actgaaacgg
     7681 tcggtctggt cgttggtgat gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg
     7741 aacaggtggc ttatcacact ggtaattttt tattgattgg caacggttac cacaacgaac
     7801 aaaaagagcg tcaggccatt gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc
     7861 atgccaaaat gatcccggat gccgatttag cctcattaat gaaacaaatg cccggtatgg
     7921 tgctgatcaa ccgtatcctg cctggctttg aaaaccgttg tattgctctg gacgatcgtt
     7981 acggtgcctg gctggctacg cgtcatttaa ttcagcaagg tcatacccgc attggttatc
     8041 tgtgctctaa ccactctatt tctgacgccg aagatcgtct gcaagggtat tacgatgccc
     8101 ttgctgaaag tggtattccg gccaatgacc ggctggtgac atttggcgaa ccagacgaaa
     8161 gcggcggcga acaggcaatg accgagcttt tgggacgagg cagaaatttc actgcggtag
     8221 cctgttataa cgattcaatg gcggcgggcg cgatgggcgt gctcaatgat aatggtattg
     8281 atgtaccggg tgagatttcg ttaattggct ttgatgatgt gctggtgtca cgctatgtgc
     8341 gcccgcgtct gaccaccgtg cgttacccaa tcgtgacgat ggcgacccag gctgccgaac
     8401 tggctttggc gctggcggat aatcgccctc tcccggaaat cactaatgtc tttagtccga
     8461 cgctggtacg tcgccattca gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca
     8521 gcgactaacc gcagttaaag caattccagc gccagtaatt cttcaatggt ctggcgacgg
     8581 cgaatcaacc gcgcctgacc attatcaaac agaacttctg gtaacagcgg acggctgttg
     8641 tagttggatg acattgatgc gccatatgcc cctgtatcat gcagtaccag ataatcacct
     8701 gccttcactt ccggcaaggc gcgggtttcg acatttcccc cttcctgctg ggtaaagaca
     8761 tcgcccgatt cacataacgg cccggcgacg acggtttcca ccgttggtgc gtgttccaga
     8821 gaacgaccat cagctgccag ggcactgata tggtggtaac taccgtacat tgccgggcgc
     8881 atcagatcgt tgaacccggc atcaaccagc acaaagtggc ggctacccat ttgtttgacg
     8941 ctccgcactt gagtaattaa tacgccagac tgcgctacca gaaagcgacc cggttcaatt
     9001 tccagtttca cagggtggcc caaatggcgg gcgatttgct gacgcgcggc attccacaga
     9061 ccataataat gctcggtatc aaccgcctct tcaccctgtt gataaggaac agaaagccca
     9121 ccgcccgcag aaatagcctg taaatcctga ccgaattcga ggacctgacg caccatagca
     9181 ccacatacct gttccagatg ggcataatcg acgccagaac caatgtgcat gtgaatgccg
     9241 accagctgta gatgatgacg ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag
     9301 ataccgtgct tgctgttttc gccaccggta ttggtttttt ggctatgccc gtgaccaaac
     9361 cccggattaa cacgcagcca tacccgatgc cctggcgaaa cctgacccag ttggtcgagc
     9421 atatcaacag aacccgcatt caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc
     9481 tgatcgataa catctgccgt aaaaacaata tcatcagggt gcgtttgcgg attgtaaccc
     9541 gccgccagtg cacgctctat ttcgcctaac gagacggaat ccactttcac gccctgctca
     9601 cgcattaagc gcaaaatatg aatattggaa caggctttct gtgcgaagcg caccacatca
     9661 aactgtttca gcgctgcaat ctgccgacga ataatttgcg catcgtagac ccacaccggg
     9721 cagccaaatt ctgcgggcaa acgcagcaga ttttcggcgg tgagatcggt atcagtgctg
     9781 aacagtgaat gtggcataac aaactccaga taagtgcttt tttatgatta cgccacatca
     9841 taaaaagaat aaaaaatatc gatttatgtc gagtctatgc aaaaatgata tggattaccg
     9901 gattgcgaga gagcgctaat ggccgccgtt aacttacgtc atattgaaat ttttcatgcg
     9961 gtaatgaccg ccggaagcct gactgaggcg gcacacctgc tacacacctc acagccaact
    10021 gtcagccgcg aactggcgcg ctttgagaag gtgatcgggc tgaaattgtt tgagcgcgta
    10081 cgtggacgat tgcatcctac cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc
    10141 tggtacggac tggatcgcat tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga
    10201 gaactgtcta ttgcctgcct gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa
    10261 ccctttctgg cacgttatcc cgatgtcagc ttaaatatcg tgccccagga atcaccgcta
    10321 cttgaagagt ggctctcggc ccagcgtcat gatttaggac tcacggagac actccatacg
    10381 cctgcgggaa cagaacgtac cgaattactc tctttagatg aagtgtgtgt gttacctccg
    10441 ggccatccgc tggcggtaaa aaaggtatta acgccggatg attttcacgg tgaaaactac
    10501 atcagccttt cccgtactga cagctatcgc cagttgctgg atcaattgtt tactgagaat
    10561 caggttaaac gacgcatgat cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta
    10621 cgggcggggg taggtgtttc ggtggttaac ccgctcaccg cactggatta tgcggcaagc
    10681 ggtttagtgg tacggcggtt cagtattgcg gttccgttca ccgttagcct gatccgcccc
    10741 ctgcaccgcc cgtcatcagc gctggttcag gcgtttagtg agcatttaca agcgggatta
    10801 ccgaaactgg tcacttctct tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga
    10861 cagcatcctc ggcatggatc gccgcagtat caaacacagg cagaacactg cgctcttctg
    10921 gcaccagtaa accaatttcc gtacagccaa aaatgacacc ctgtgcgccc tgttctgcaa
    10981 ggcgagcaat cacttgcgca taataagtgc gtgacgcttc ggtaaattgc cccagacaca
    11041 gttcttcaaa aataatctga ttaattttcg cccgtttatc cgcttcagga atgagacagt
    11101 tgatggaaaa ttgttccgtc agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg
    11161 tacccagcag cgccacacga gtcattccag cccggataat tgcacgtcca gtggcatccg
    11221 ctatgtgtaa gaaaggcaga gagcaacgag actcaatggc atccgccact ttatgcatcg
    11281 tattggtgca taacacaata ccttctgcgc ccgcccgctg taagccaagc gccgcctcag
    11341 ccagaatatc cccggttttg tcccactcac cgcgacgctg gcactcttct atttcatgaa
    11401 aatcgacgct atgtagcagc acttgcgcag agtgaagccc accaagccgc tgtttaatgc
    11461 cttcatttat cagacgatag taaggaatag tggattccca gctcattcct cccagcaaac
    11521 caattgtttt cattcctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
    11581 agacaaacgc ctcaacggcc aggtgcctaa tctctattaa cgaaaaaagg gccggatgta
    11641 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttttcgc ctgccatcag
    11701 tttgcgctcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
    11761 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag cgccaatgct
    11821 atcaagcagt gtcaggaagg tcgcgccgat aatcatattc gacacccagt ttgtcgtcgt
    11881 tgagcaggtg atgccaaaat cgcggcattt cagcggctga atttcagagc acaagatcca
    11941 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
    12001 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc agtaacccag
    12061 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggct tacgccctgc
    12121 tttatctacc gtaaagaccg caatgaaggt agcgaacata aaggtcagcc ccacgaccag
    12181 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgtggcgc
    12241 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
    12301 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
    12361 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    12421 catacgcaac acttcttccg cctcaatatg acgccctttt tccgccagcc agcgcgggct
    12481 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    12541 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    12601 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    12661 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    12721 cacacgagcg gcgattaaca tctctacgct ggtcgcaaat gcggacccta tcgaaccgag
    12781 cacaaaaagg atagcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    12841 cccattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactgacca cccattcctg
    12901 caaacggctg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    12961 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    13021 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    13081 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaat tcacacaggt
    13141 gtcaggtcgg aaatagcata aatatggatt aaattgctgc g
//
LOCUS       DAZUCC010000140        13003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZUCC010000140 DAZUCC010000000
VERSION     DAZUCC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.141"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(164..1276)
                     /gene="ydiK"
                     /locus_tag="ACHY09_003448"
     CDS             complement(164..1276)
                     /gene="ydiK"
                     /locus_tag="ACHY09_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFS3886377.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            1663..4719
                     /locus_tag="ACHY09_003449"
     CDS             1663..4719
                     /locus_tag="ACHY09_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFS3886378.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSSMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWVAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4716..5126
                     /gene="menI"
                     /locus_tag="ACHY09_003450"
     CDS             4716..5126
                     /gene="menI"
                     /locus_tag="ACHY09_003450"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFS3886379.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5226..5414
                     /locus_tag="ACHY09_003451"
     CDS             5226..5414
                     /locus_tag="ACHY09_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFS3886380.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            5963..6331
                     /gene="sufA"
                     /locus_tag="ACHY09_003452"
     CDS             5963..6331
                     /gene="sufA"
                     /locus_tag="ACHY09_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFS3886381.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6340..7827
                     /gene="sufB"
                     /locus_tag="ACHY09_003453"
     CDS             6340..7827
                     /gene="sufB"
                     /locus_tag="ACHY09_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS3886382.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7837..8583
                     /gene="sufC"
                     /locus_tag="ACHY09_003454"
     CDS             7837..8583
                     /gene="sufC"
                     /locus_tag="ACHY09_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS3886383.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8558..9829
                     /gene="sufD"
                     /locus_tag="ACHY09_003455"
     CDS             8558..9829
                     /gene="sufD"
                     /locus_tag="ACHY09_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS3886384.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9826..11046
                     /gene="sufS"
                     /locus_tag="ACHY09_003456"
     CDS             9826..11046
                     /gene="sufS"
                     /locus_tag="ACHY09_003456"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFS3886385.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11059..11475
                     /gene="sufE"
                     /locus_tag="ACHY09_003457"
     CDS             11059..11475
                     /gene="sufE"
                     /locus_tag="ACHY09_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFS3886386.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11624..12628
                     /gene="ldtE"
                     /locus_tag="ACHY09_003458"
     CDS             11624..12628
                     /gene="ldtE"
                     /locus_tag="ACHY09_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFS3886387.1"
                     /translation="MKCASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKGVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12691..12927)
                     /gene="lpp"
                     /locus_tag="ACHY09_003459"
     CDS             complement(12691..12927)
                     /gene="lpp"
                     /locus_tag="ACHY09_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFS3886388.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3264 a         3205 c         3485 g         3049 t
ORIGIN      
        1 ctttttgcgt cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg
       61 tactaccagc tgaatggcgg taagcaggaa aatatgggga agagcaagca aatatcaatt
      121 agcaggggtt aacaatcagg cggcagagcc gcctgatgag aaattactta ttcgatttct
      181 cgatttcgcc aagttcttcg agaatttctt ccggttggtc cgtcggcggt gggacttctt
      241 ccacccacgc ggcaaaaaga cgccaggaaa cggctaacag aaccggacca ataaacaggc
      301 cgatcatccc gaaagcaatc aaaccaccaa taacaccaga gaggatcagg atcagcggta
      361 aatcggcacc catgcgaatt aacattgggc ggatgacgtt atccagcgtg ccaaccacac
      421 cgctccacac taacaatacc gttccccagg tggtatcacc ggtccagtag agccagataa
      481 tcgccggaat cagtaccggc aacggtccaa gctggacaag gcaggagagg atcattaaca
      541 ccgtgagcaa agttgcataa ggtacgccgg atacggcgag gccgataccg ccaagcactg
      601 cctgtactaa cgccgtcacc accacaccca gcgccaccgc gcggatagcc tgtgccgcca
      661 gtagcacagc agcatcaccg cgaacgcctg ccagacgggt tgcaaaatgg cgaatgcctt
      721 gtgccacctg ttcaccgcgc cagtacagca gggcactgaa gagaagcatc agcgcacaat
      781 gcaccataaa gcgcccgata tgcgccgcct gcccaacgaa ccaggtggtg gtggtgccaa
      841 tataaggacg gactttcgcc atgatcgccg taccccccat atccagcaag ttgtgccagc
      901 ctgcatatag cttcgcgcca atcaccggaa tggtattaag ccacgccaga tcgggtaacg
      961 tcatgtcacc gctggaaatg gctttaatta gcggaccgct gccatcgaca atactgttaa
     1021 ccaacaaggc aatagggatg ataaacacca tcactaataa cagcgtcatc accagaacgg
     1081 cgagagagcg gcggccaaac atgatctttt gcaaacgtaa caataccggc caggtggcga
     1141 taaccactgt accggcccat gcaaagccga gaataaaggg ttgaacaatc cacagacatg
     1201 ccacaatcat gatggctaaa aacagcaccg aaagcagaat ttgtgcgaca tccctgggct
     1261 gacgaacatt taccataact acttttcacc ttttttgtgc gtcaaatcgt cgacgcgacg
     1321 tgaacacgcg aaactcacct gcataatgat gagcaatttc agcggttttt aacaggtcga
     1381 ttctgccctt aattctgatg ggcgtgtaag aaaaaaatgt ggtacaacaa ctcaggcaac
     1441 acgcaaacga tttactcgtc gtatttcaaa ttgcatgtgc tgcggctacg ttcgttcacc
     1501 ccagtcactt acttttgtaa gctcctggag ttcactcgct tgccgccttc ctgcaactcg
     1561 aaatccatag agtaaacaaa catcaccttt cagtgcgcta ttagcagcct gaaagaacag
     1621 tgtatcgatc tacatcacag accgcaggaa agggtcaata taatgattcc acagatttcc
     1681 caggcacccg gcgtcgttca attggtgctt aattttttgc aagagctgga gcaacagggt
     1741 tttactggcg atacggcgac aagttatgcc gatcgtctga caatgtcgac cgacaacagt
     1801 atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga tgtggcgctg
     1861 atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc ccgcggcggc
     1921 ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat gtcccgccat
     1981 atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga ggccggggtg
     2041 ataaaagatc aactcaatca gtacctgaaa ccgttcggct acttttttgc gccggaactt
     2101 tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc cggtcaggga
     2161 tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt gttgttgggg
     2221 ggcgatattc tcgatacgca acctttaccc gtcgaactgg cggaaacgct gggtaaatcc
     2281 aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca gcaacgccag
     2341 ttaatcatcg acaacttccc caagcttaac cgctttctta ccggttacga tctgcgtcat
     2401 gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg
     2461 acgctggcct ttattaccga agcgcgactg gatattacgc gcttgcctaa agtgcgccgt
     2521 ctggtgaacg tcaaatatga ctcttttgac tccgcactgc gtaacgcgcc gtttatggtt
     2581 gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct ggcgcgggaa
     2641 gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa agaaatgctc
     2701 gggctgaaca ttgtggaatt tgctggtgat gatgagacgc tgattgatga gcgggtaaat
     2761 gcactctgtg cgcggttgga tgaactgatt gccagccaac aggcaggtgt gattggctgg
     2821 caggtgtgcc gcgagctggc gggtgttgaa cgtatctatg cgatgcgcaa aaaagccgtt
     2881 ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga ggatacctgc
     2941 gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct cgacagccac
     3001 ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt ccgtcctgcg
     3061 ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga tgacgtagtg
     3121 gcgcttaccg cgaaatacgg tggtttgttg tggggcgaac acggcaaagg ttttcgtgcc
     3181 gaatacagcc ctgcgttttt cggtgaggaa ctttttgcag aactgcgcaa agtgaaagcg
     3241 gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga aggtctcgat
     3301 gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca gatccccatt
     3361 gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt atgcttcaac
     3421 tttgatgccc gtagttcgat gtgtccgtcg atgaagatca cccagaaccg gattcattca
     3481 ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga tcgcggcgtt
     3541 gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg gacgttaatt
     3601 gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat acgacttctc acacgaagtc
     3661 aaagaggcga tgtcgggctg tcttgcctgt aaagcgtgtt cgacccagtg ccccatcaaa
     3721 attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg ttatttacgc
     3781 ccgcttcgtg accacctcgt tgctacggtc gagagctacg caccgctgat ggcacgcgcg
     3841 ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaagctctc ggaaaaacat
     3901 atcggcatgg ttgatttgcc actgctgtca gtcccctcgc tacaacaaca aatggtgggg
     3961 catcgctcgg cgaatatgac gctggaacag cttgaagctc tcaatgcaga gcagaaagcg
     4021 cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca agtggtggcg
     4081 gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc attttcgcca
     4141 aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac ggcgaaaaag
     4201 acggcggatt tcctcaaccg tatggcgaag ctggggatgc caatggtggg cgtcgatccg
     4261 gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga acgtggcgag
     4321 tttaacgtct tactggcgaa tgaatggctg gcaagcgcac ttgactcaca gccagtggct
     4381 acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaagttac cgctttgccg
     4441 ggcgcgccag cacaatgggt tgcgatattt gcccgttttg gcgcgaaact ggaaaatgtc
     4501 agcgtggggt gctgcggcat ggcagggact tacggacatg aagcgaaaaa ccataaaaat
     4561 tcgctcggga tctatgagtt atcctggcat caggcgatgc agcgactgcc gcgtaatcgc
     4621 tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggtcgaagg cacgggggta
     4681 cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacga aaaatcaccc
     4741 tggaagcact gaatgccatg ggtgaaggaa acatggtggg gttgctggat attcgctttg
     4801 aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg acaaagcagc
     4861 ctttcgggtt gctgcatgga ggggcatccg tggtactggc cgaaagtatc ggttccgttg
     4921 ccggttattt atgtaccgaa ggtgagcaaa aggtggttgg tctggaaatc aatgctaacc
     4981 acgtccgctc ggcacgagaa gggcgggtgc gcggcgtatg caaaccgttg catctcggtt
     5041 cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg tgctgttcgt
     5101 cacgattaac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt
     5161 ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct aaaagcgagg
     5221 atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta cgccgtgacc
     5281 agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa cttgaatttg
     5341 ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt gcctggcgtc
     5401 tgggcgtgca ttgatcctga cttaatgccg caattttgcc aaatcgacct ggattaacct
     5461 ccgtccaggt tcgatgacct tcttaatact gcttatctat ccgcctgata ttgttcaaaa
     5521 ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat tattcttatc
     5581 gccccttcaa gagctaagcc actgtgagtg ccggagataa gcgccggatg gggtagaaac
     5641 ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct gcactttttt
     5701 acctatccct taaatagtca ttttcaatac aactttttct ttttcatcac agccttaatg
     5761 ctccttagat aataatcatt atctaacaat gagataccta attcttagcg tgcctgttaa
     5821 cccacacatc agggtctatg cttattaaaa gcaggtaaaa agcatgtttt acgctaaagt
     5881 ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt atacgctgaa
     5941 agcgatgaag tgaggtaaat cgatggacat gcattcagga acctttaatc cacaggattt
     6001 cgcctggcag ggcttaacgc tgacacccgc agccgctgtc cacatccgcg agctggtggc
     6061 aaagcagccg ggtatggtcg gcgtgcgctt aggcgtgaag caaacgggct gcgcgggctt
     6121 tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt ttgaacacga
     6181 cggcgcgaag ctgtttgtcc cgctgcaagc gatgccgttt attgatggca cggaagtcga
     6241 tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag cccagaatga
     6301 atgtggctgt ggcgaaagct ttggggtata ggcggtacta tgtctcgtaa tactgaagca
     6361 actgacgatg tcaaaacctg gaccggcggc ccgctgaatt ataaagaagg attcttcacc
     6421 cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg cgcaatttcg
     6481 gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaatgccta tcgcgcatgg
     6541 ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa ttatcaggat
     6601 tacagctact attcggcacc atcgtgcggt aattgtgacg acacttgtgc gtctgaaccc
     6661 ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga ggcggcgttt
     6721 gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cagtggatgc cattttcgac
     6781 tctgtttcgg ttgccactac ttatcgcgaa aaactggcgg agcagggaat tattttttgt
     6841 tcctttggcg aggcgatcca cgatcacccg gaactggtgc gtaaatatct cggcaccgtg
     6901 gtgccgggga atgacaactt ctttgccgca cttaatgcgg cggtagcctc tgacggtacg
     6961 tttatttatg tgcctaaagg cgtgcgttgc ccgatggaac tttccaccta ttttcgcatt
     7021 aacgcggaaa aaaccgggca gtttgagcgc accattctgg tggccgacga agacagctac
     7081 gttagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt acacgcggcg
     7141 gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt acaaaactgg
     7201 ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg tgcgttgtgc
     7261 gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc gattacgtgg
     7321 aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta ctcagtggcg
     7381 ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat cggtaaaaac
     7441 accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa cagttatcgc
     7501 ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca gtgcgattca
     7561 atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg tcgcaataac
     7621 agcgctcagc tggagcacga ggcaacgaca tcacgtattg gtgaagatca actgttttac
     7681 tgcctgcaac gcgggatcag cgaagaagat gccatctcga tgattgttaa cggtttttgc
     7741 aaagacgtgt tctcggaact gccgctggaa tttgccgttg aagcacaaaa actcctcgcc
     7801 atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa agatttacac
     7861 gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg tcccggcgaa
     7921 gttcacgcca ttatggggcc aaacggttct ggcaaaagta ccttatcggc aacgcttgcc
     7981 gggcgagaag attatgaggt gacgggcggc acggttgagt tcaaaggcaa agatttgctt
     8041 gcgctgtcac cggaagatcg tgcaggtgaa ggcatcttta tggccttcca gtatccggtg
     8101 gaaattcccg gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc ggtgcgcagc
     8161 tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga agagaaaatc
     8221 gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg tttttccggc
     8281 ggtgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga gttatgcatt
     8341 cttgatgagt cagactccgg gctggatatt gacgcattaa aagtggttgc cgatggcgtg
     8401 aactcgctgc gtgacggcaa gcgttcattc atcattgtta cgcactacca acgcattctc
     8461 gactacatca agcctgatta cgtccatgtg ctgtatcagg gacgaattgt gaaatccggc
     8521 gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac cgaacagcag
     8581 taacgcactg caacagtggc atcatttgtt tgaagctgaa ggggcgaaac gctccccgca
     8641 agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta aacatgaaaa
     8701 ctggaaatat acgccgctgg acgggctgac caatagccag tttgtcagca ttgcggggga
     8761 gatatcccca cagcagcgtg atgccttagc gttaacatta gacgccgtgc ggctggtgtt
     8821 tgtcgatgga cgttacgtgc ccgcactgag cgatgcaact gaaggcagcg gatatgaagt
     8881 gagcattaac gacgaccgtc aggggttacc cgatgctatt caggcggaag tgtttctgca
     8941 tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg gtcaacggcc
     9001 ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag aggtgaatac
     9061 tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca
     9121 ttttgtcagc cttaatgatg ctcgtcactt caccggcgca cggttcacta tcaacgtcgc
     9181 agcgaacgcc catttgcagc atatcaagct ggcgtttgaa aacccgctca gtcaccactt
     9241 tgctcataac gatttgttgc tggctgacga tgccaccgca tttagccaca gtttcctgct
     9301 gggtggcgca gtattacgac acaacaccag tacgcaactc aatggcgaaa acagcacgct
     9361 gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgatactc gtacctggtt
     9421 ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg tcagcgacaa
     9481 aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca aaacggatgg
     9541 tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata cgaaaccgca
     9601 gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg ggcgtattga
     9661 tgacgaacag atgttttatc tgcgctcgcg cgggatcaat cagcaagatg cccagcagat
     9721 gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg ggcttaaaca
     9781 gcaggtgctg gcccgaatcg gtcaacgact gccaggaggt gcaagatgac tttttccgtc
     9841 gacaaagtgc gggccgactt tccggtgctt tctcgtgagg taaacggttt gccgctggct
     9901 tatctcgaca gcgccgccag tgcgcagaaa ccgagccagg tgattgatgc cgaggctgag
     9961 ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag cgcccaggcg
    10021 accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg
    10081 gaagagctgg tgttcgtccg cggcacgacg gaagggatca atctggtcgc caatagctgg
    10141 ggcaacagca atgtgcgagc gggcgataac atcatcatca gtcagatgga gcaccacgct
    10201 aacattgtcc cctggcagat gctttgtgca cgcgttggcg cagagctgcg tgtgatcccg
    10261 ctcaaccccg acggtactct gcaactggag acgctgccta cgctgtttga tgagaaaact
    10321 cgtctgctgg caattactca tgtctccaac gtgcttggga cagaaaatcc gctggcggag
    10381 atgatcacac ttgcgcatca acatggcgca aaagtgctgg tggatggcgc tcaggcggtg
    10441 atgcatcatc cggtggatgt tcaggcgctg gattgcgatt tttacgtgtt ctccgggcat
    10501 aaactgtatg gccctaccgg aattggcatt ctttatgtca aagaagcctt gttgcaggag
    10561 atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag tgaaggcact
    10621 acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg aggcatcatt
    10681 ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaataacat agccgagtat
    10741 gaacagaatc tgatgcatta cgcgttatca cagttggaat ctgtaccgga tctcactctc
    10801 tatggcccac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca ccacgcctat
    10861 gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca tcactgcgca
    10921 atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcatcgct ggccatgtat
    10981 aacacccatg aagaagtgga tcgtctggtg accggcctgc aacgtattca ccgtctgctg
    11041 ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct gcgtaatttt
    11101 ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagttggg tcagcgtctg
    11161 ccagaattac gtgacgaaga caaaagcccg caaaatagca tccagggctg ccagagtcag
    11221 gtgtggattg tgatgcgtca aaatgcgcag ggaattattg aattacaggg cgacagcgat
    11281 gcggccattg taaaagggct tattgcggtc gtctttattc tttacgatca aatgacgccg
    11341 caggatattg tcaatttcga tgtgcgcccg tggtttgaaa aaatggcgct cacccaacat
    11401 ctcaccccat cacgctcaca aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc
    11461 gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat tgccgggtgg
    11521 tcaatgttta cactggcctg cacaggagtg ttttggtttc aggtgaacat aagcgccatc
    11581 cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaat gcgcgtcttt
    11641 gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg cggttgatta
    11701 tccgctacca ccaaccggca gtcgactggt tgggcaaaat caaacgtata cggtgcaaga
    11761 aggggataaa aaccttcagg ccatcgcccg acgttttgat actgcggcaa tgttgatcct
    11821 tgaagccaat aacactatcg ccccggtgcc aaaacccggt acgacgataa ctattccttc
    11881 acaactatta ttacctgatg cgccgcgtca ggggattatt gtaaaccttg cagagctgcg
    11941 cctttataat tatccgccgg gagaaaatat cgtgcaggtt tatccgatag gtattggttt
    12001 gcaggggctg gaaacgccgg tgatggaaac gcgtgtgggg cagaaaatcc ctaacccaac
    12061 ctggacgcct acggcgggca ttcgtcaacg ttcgctggaa cgtggcatta aattaccacc
    12121 agtcgttcct gccgggccca ataacccact aggacgttac gcactgcgcc tcgcgcatgg
    12181 taatggcgaa tacctcattc acggcaccag tgcgccggac agcgtcggtt tgcgcgtcag
    12241 ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttattctcca gcgtgcggac
    12301 gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggagc ctaacgggat
    12361 gcgttatgtt gaagtacatc gaccactatc ggcagaagaa cagcagaacg ttcagacaat
    12421 gccatacaca ctgccagcag gctttacgca atttaaagac aataagggtg tagatcagaa
    12481 attagtcgat aaagcgttgt atcgtcgggc agggtatccg gttgcggtga gcagtggagc
    12541 aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg aaccagagca
    12601 agggaatatg ttacgcgcga cgcagtagcg gtaaatggca ggcaaaaaaa tggcgcacaa
    12661 tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag ccatgttgtc
    12721 cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg aacgcattgc
    12781 gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt cagaagacag
    12841 ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca ggattaccgc
    12901 gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt taatctccat
    12961 gtagcgttac aagtattaca caaagttttt tatgttgaga ata
//
LOCUS       DAZUCC010000141        12985 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZUCC010000141 DAZUCC010000000
VERSION     DAZUCC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.142"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(62..769)
                     /locus_tag="ACHY09_003460"
     CDS             complement(62..769)
                     /locus_tag="ACHY09_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3886389.1"
                     /translation="MAIVKLKPQEAHMKHAVKVMDEIAAQIQEGGSLLGMIYSYMSEN
                     GAESERVYNAIRCLMRSLQKTEETASIFAEKLCEAKAMVSYGHFFGKIRAQREACGLT
                     TTELARLLDLDEEIILQWESGEFEPTISMLIPLANVLGCDPLSLLDEKDSASVIRVNA
                     PEPREESIGARIKSARKKLGLTEADLARMIHTYSDPINDWECGVCEVPADQIVPLASA
                     LNCDLMWLLTGKSLAKE"
     gene            complement(910..1614)
                     /locus_tag="ACHY09_003461"
     CDS             complement(910..1614)
                     /locus_tag="ACHY09_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS3886390.1"
                     /translation="MSFSDRLQLAMTIRGYSQGKLAREVGMAQSSVNKLVNGATGSRK
                     VVEIANILNVRPEWLSYGVGPMTPEESTSCNPTPQVSYSPLENDKDVFRVEVLDIAAS
                     AGPGTFLVSDFAETVHAIEFSHDAARRLFCSRPANIIKMITVDGDSMAPTLCAGDQVF
                     VDVSVRNFETDGIYIFIFGHTFHIKRLQMQGMQLAVISDNPAYKEWFISESAEEHLFI
                     MGKVLIHQSIQYNRVG"
     gene            1738..2001
                     /locus_tag="ACHY09_003462"
     CDS             1738..2001
                     /locus_tag="ACHY09_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3886391.1"
                     /translation="MKNEGIAKAVDIAGSQIALARRCGRAQSTICDWLNGKKKISPEF
                     VPSLVKAVDGKVQAYEFRPDLPELFPHPGLAHTEDGVYEGDKQ"
     gene            1998..2705
                     /locus_tag="ACHY09_003463"
     CDS             1998..2705
                     /locus_tag="ACHY09_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS3886392.1"
                     /translation="MNTTIFTDKASMTSIEIAELVGSRHDSVKRAIERIAERCVTQLP
                     PMVEVENKQSLSPNKYTKAYIFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     RPMSQIEMVAAMALEAVRQQRRLEQVEEKVTHVTETVEQIKRGTIRDGYAGYRQLVAK
                     TGMSDAKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFRHMMSEAEPRGT
                     RWYHPKMGLFQALGWQV"
     gene            2725..2895
                     /locus_tag="ACHY09_003464"
     CDS             2725..2895
                     /locus_tag="ACHY09_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS3886393.1"
                     /translation="MQVLNRWFRDGRGRRVHVIRWEPETQRVIYLRDGYPDECFSPLW
                     LFRRDFVECEAP"
     gene            2834..3550
                     /locus_tag="ACHY09_003465"
     CDS             2834..3550
                     /locus_tag="ACHY09_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFS3886394.1"
                     /translation="MSASVRCGCFVVILLSAKRRKACAVYNIDFRRYTFLIVAKSTIG
                     SETPELIEATNDAPASFLLSVPAHTRIMVGWAGEPKGSPVSLIAGSLNPVQSATSEIE
                     TSCGGYCHSPIKEAAIMATIPAHPYLKIEVVNGRAVIFSLYVACHFKRMHKNIVDRIE
                     YLNCSREFLTRNFIPGTYRAYGDSVRGYYITRDGLMMLQLGLSLRTMRYYESCIKALH
                     EVETFQGHSAFRRNQREVHQ"
     gene            3547..4410
                     /locus_tag="ACHY09_003466"
     CDS             3547..4410
                     /locus_tag="ACHY09_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS3886395.1"
                     /translation="MKRHQHIDSAIPERYTVKAPYKAGAGIGVLKLITEQNRAHAVFS
                     YHEHCYAQIMVGRTGPTSVGPGSVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMT
                     TLPTLVQPEIAIVNGQAVTTSLAVADFFSKRHDDVLKKIRILDCSSEFCARNFAETSI
                     SLNQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAA
                     DMSSVAQNARGVYLHLREIHQIWKSQLYPMLKAVESPLASKLYDRVGDAVFGAALVDS
                     RLNGSDKEVRP"
     gene            4407..4631
                     /locus_tag="ACHY09_003467"
     CDS             4407..4631
                     /locus_tag="ACHY09_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886396.1"
                     /translation="MIRRVVNFLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQKVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGAV"
     gene            4628..4954
                     /locus_tag="ACHY09_003468"
     CDS             4628..4954
                     /locus_tag="ACHY09_003468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886397.1"
                     /translation="MICKLTPEKKALNKSSRLLLSYLQNASGEQGFVEISLLELSAAL
                     GLCRNTITNATALLEDLGVVTVSRGRRNKTKSGKESAGKNIYRLIQSATNNKDYAIVM
                     PRKEVV"
     gene            4951..5943
                     /locus_tag="ACHY09_003469"
     CDS             4951..5943
                     /locus_tag="ACHY09_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS3886398.1"
                     /translation="MSRYAPTPEVMAIGQINISGNVTPPSWWKHIQLPSGRPDATAIA
                     LLSEIVYWYRPTEVRDEHTGALLGYRKRFQSDKLQRSYQAFAEQFGFGKRETADALKR
                     LRDAGFITLDLRTVEMLDGVKCSNILFVGINPQAIAAITTPSSVSPESNSNNAISDTA
                     ITLKRNTPQRHNGTGDTPNVDTNTEITTEITTETKNTIGASADASAPARSARQEYSPE
                     FEQAWQEYPKRAGGNSKSAAFKAWKARIREGVTPETMLNGVKRYAAWVRVSGNIGTQF
                     VKQASTFFGPDRHFDESWQQPAVPGGGRQRQVDVLSGLGAMSDKFGKSSDKLTF"
     gene            5954..6832
                     /locus_tag="ACHY09_003470"
     CDS             5954..6832
                     /locus_tag="ACHY09_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS3886399.1"
                     /translation="MMAANQREKQTRLQARMDELRSEMAFAKSGEKPWPYRSCLIREG
                     RGYCEKHGEYHTHTLVWGDRNGEDREKVSRCPDCLSAEINDVMMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMTKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSVAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWASYRSRIGGVAA"
     gene            6829..8229
                     /locus_tag="ACHY09_003471"
     CDS             6829..8229
                     /locus_tag="ACHY09_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS3886400.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFF
                     VRQYRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMATLSTGKGVIQPVHIDDVL
                     PEVVNRVEFRSQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSIGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPDTQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMPGSKNGRLF"
     gene            8226..8483
                     /locus_tag="ACHY09_003472"
     CDS             8226..8483
                     /locus_tag="ACHY09_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886401.1"
                     /translation="MNNRTITVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            8536..9525
                     /locus_tag="ACHY09_003473"
                     /pseudo
     CDS             8536..9525
                     /locus_tag="ACHY09_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            9543..9968
                     /locus_tag="ACHY09_003474"
     CDS             9543..9968
                     /locus_tag="ACHY09_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFS3886402.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CSDDDAMIICGCMARLYRNNSDLHDLLVDYYVLGMTLMSLGRKHERSDCWVGRVLQKA
                     EGVIEGMLMALDVKLEMDPYVEREPSGTSTDQFAGQTEN"
     gene            10146..10301
                     /locus_tag="ACHY09_003475"
     CDS             10146..10301
                     /locus_tag="ACHY09_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3886403.1"
                     /translation="MKQQKVMLIALIVICITVVVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            10858..12570
                     /locus_tag="ACHY09_003476"
     CDS             10858..12570
                     /locus_tag="ACHY09_003476"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFS3886404.1"
                     /translation="MNGAVHYANALLQFTSYPDIIAVGMTGWRDDDTGELHHEIGVWH
                     VSKNNLGAGQKVGEYADLSFLSASNFDDFINKVSLLNLSPEELEKIKASKEEEIDTRL
                     SRLNNDIYQNEKGLGENDRVYLVVATVIATLGIPGKLTPLDKSELVSSTEEGERDGDI
                     IFRKVKNFLAIKGVPETKRGIILRTLQNTLWTENINKPVNGESQLKRVFIKVVDDLGE
                     YYKIGLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLAKLARVNKDSYVWDF
                     ATGSAGLLVAAMNEMLTDARDKIKSPDELKLKEVQIKAEQLLGLEVLSSIYMLAVLNM
                     ILMGDGSANILNKDSLADFDGRYGFGKTDEKFPATAFVLNPPYSAKGNGMIFVQKALS
                     MMHSGYAAVIIQGSAGSGKAKEYNQKILQEHTLLASIKMPADLFIGKSSVQTYVYVFH
                     VKQAHNPKTLVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKNCLS
                     IFTEDEYFEATIDPVKGDDWNQTKPVDTRPTLTDFKKTVGDYLAWEVSQLLKKQGEND
                     FAGK"
     gene            12639..>12985
                     /locus_tag="ACHY09_003477"
     CDS             12639..>12985
                     /locus_tag="ACHY09_003477"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS3886405.1"
                     /translation="MSTGSLISSERLKPGKIKRVSAKSDQNGIIGEFDTEFMDDARHY
                     ENFISVNFFGDVFYHPYCASVEMKVHVLTLKQSKSGFNAKCGLYVASMLNKALRGRFN
                     YGQQLSSSKLRDG"
BASE COUNT         3382 a         2930 c         3420 g         3253 t
ORIGIN      
        1 aaggaatcag gtgccaggca taaaccttca tacttatcat ggatattgcc agtcattgct
       61 gctactcctt tgccagggat ttaccggtta acagccacat caggtcacag ttaagtgcgc
      121 tggccagagg tactatctga tcggcaggaa cttcgcacac gccgcattcc cagtcgttta
      181 tggggtcgct gtaggtgtga atcatgcgtg ccaggtctgc ttctgttaag cccagttttt
      241 tacgagcgct tttgatgcgc gcaccaatac tttcttcccg tggttctggc gcatttacgc
      301 gaataacaga cgcgctatct ttttcatcca gcaaagaaag cggatcgcac ccaaggacgt
      361 ttgccagggg gataagcatg ctgatggttg gttcgaattc tccgctctcc cattgcagga
      421 tgatctcttc atcgagatca agcagtctgg caagttcggt ggtcgttaag ccgcaggctt
      481 cacgttgggc gcggattttt ccaaaaaagt gaccatacga aaccatagct tttgcctcac
      541 atagtttttc tgcaaaaatt gatgcagttt cttctgtttt ttgcagagaa cgcatcaggc
      601 atctgattgc attataaacc ctctcacttt ctgctccgtt ttctgacatg taggaataga
      661 tcattccaag cagcgaaccg ccttcttgta tttgtgctgc aatttcatcc atcaccttaa
      721 ctgcatgctt catatgagcc tcctgtggtt ttaattttac aatagccaaa gcgtttagtt
      781 atgtcaatcg ctatagctat gtgttttggg cagcaaagaa cagattatgc gagaaatgag
      841 acgatacgta gggagttgag atacaggaaa gtagagcagc tgcgcaatac agctgccggt
      901 gatgttgact tatccgaccc tgttatactg gattgactga tgaatgagaa ctttccccat
      961 tatgaataga tgttcttcag ctgattcact tataaaccat tccttgtagg ccggattgtc
     1021 tgatataaca gcaagttgca tcccttgcat ttgtaggcgt ttgatatgga aggtgtgccc
     1081 aaaaataaaa atatagatac cgtcagtttc aaaatttctg acggatacgt cgacaaatac
     1141 ctgatctcct gcacacagag taggagccat gctgtcgcca tcaactgtta tcatctttat
     1201 gatatttgct ggcctgctac agaacaggcg tcttgcagca tcatgtgaaa attctatggc
     1261 atggaccgtt tctgcaaaat ctgacacaag gaatgttccc ggccctgcgc tggctgctat
     1321 atccaggacc tcaacacgaa aaacatcctt atcattttcc agtggggaat atgatacctg
     1381 tggtgtcgga ttgcaggatg tgctttcttc tggggtcata ggaccaacac cgtacgatag
     1441 ccactcgggg cgcacgttca ggatattggc tatctccaca actttcctgg agcctgtggc
     1501 accattaacc agtttattga cgctcgattg agccatgcca acctctctgg ccagttttcc
     1561 ctgcgagtat ccgcggatag tcatagctaa ctgtagcctg tctgaaaagc tcataagtcc
     1621 tcctttttct gcccttttag tgtatcgcct tagcgatata tacacaaacc gctttttcgt
     1681 acattgcaat cgcttaaatc aatcgctata gtgaataaaa tcaggttgga ggtcgatatg
     1741 aaaaatgaag gtatagccaa ggctgtcgat attgcgggaa gccagattgc tctggccagg
     1801 cgttgcggtc gcgcgcagtc aacaatttgc gactggctga atgggaagaa aaaaatttct
     1861 cctgaatttg ttccgtctct ggttaaggcg gttgatggga aagtccaggc gtatgaattt
     1921 cgtcctgatc taccggagct tttcccacac ccaggcttag cgcacacaga agatggagtg
     1981 tacgaaggag ataaacagtg aatacaacaa tttttaccga caaagcatcc atgaccagca
     2041 ttgagattgc cgagctggta ggtagccgcc atgacagtgt gaagagagca attgaacgta
     2101 ttgctgaacg atgtgttacc caacttccac caatggtgga agttgagaat aaacaatcac
     2161 ttagtcctaa taaatatact aaggcgtaca tcttcgaagg cgaacaaggc aaacgcgaca
     2221 gcatcattgt tgtcgcacag ctttgccctg aattcaccgc tcgcctggta gaccgctggc
     2281 gcgaactgga agaacaaatc cgcagaccaa tgagccagat cgaaatggtt gctgcaatgg
     2341 cgcttgaagc cgttcgccaa caaagacgtc ttgagcaggt ggaagagaaa gtcacccacg
     2401 ttactgaaac cgtcgagcaa atcaaaagag gcaccatacg cgatggttat gcaggctatc
     2461 gtcagctggt ggcgaaaacg ggtatgtcag atgcgaaatg ccgcaatctg gtgaacgcat
     2521 atcagatccc taccgacacc catgaattta tgacaccaga tggtttgttg tcgcgtcgcg
     2581 cgattgttgc agtagaacct ttcatggctg ctttccgtca catgatgagt gaagcagaac
     2641 cacgaggtac tcgctggtat cacccgaaaa tgggactgtt ccaggcgctg ggctggcagg
     2701 tgtgattatg ctcacaggta acacatgcaa gtactcaatc gctggttccg tgatggaaga
     2761 gggcgtcgcg tccacgttat acgctgggag cctgaaacac agcgggttat ttacctgcgt
     2821 gatggttatc cggatgagtg cttcagtccg ttgtggctgt ttcgtcgtga ttttgttgag
     2881 tgcgaagcgc cgtaaggcct gtgctgttta caatattgat ttccggcggt acacttttct
     2941 tatagtggca aaatccacta tcgggagtga gaccccggaa ttgatcgagg cgacaaacga
     3001 cgcgccagcg tcttttttgt tgtcagtgcc tgcgcacacc agaattatgg tgggctgggc
     3061 aggggaaccg aaaggttcgc cggtttcctt gatcgccggt agtctcaacc ctgtccagtc
     3121 cgccaccagc gagattgaga cctcctgtgg tggatattgt cattcaccga tcaaggaggc
     3181 tgccattatg gctacgatcc ccgcacaccc atacctcaaa atcgaggtcg tcaacggcag
     3241 agccgttata ttctccctgt atgtcgcctg tcactttaag cgcatgcata agaatatcgt
     3301 cgatcgaatc gaatatctca actgctcacg cgaatttctt acccgcaact ttatacctgg
     3361 cacttatcgc gcctatggcg attcagtgcg cggttattac atcacccgtg atggtctgat
     3421 gatgctacag ctagggttaa gtctgcgcac catgcggtac tacgagagct gcattaaagc
     3481 actacatgag gttgaaacct tccagggcca ctctgctttc cgtcgtaatc aacgggaggt
     3541 gcatcaatga agcgccacca gcatattgat tctgcaattc cggaacgtta cactgtgaag
     3601 gcaccttata aagcgggtgc cgggattggc gtcctgaaat tgattactga gcaaaaccgc
     3661 gctcatgcgg ttttttcgta ccatgagcat tgctacgccc aaattatggt ggggcgtaca
     3721 gggccgactt cggtcgggcc gggttcggta gtctccggta acgccaaccc tgtacgtctc
     3781 accacctctg tgattggcgt cccatgtggt gagttttcta aaaaactgac taccggggct
     3841 gtcaccatga ctactctccc aaccctcgtt caacctgaaa ttgccattgt taatgggcag
     3901 gctgttacaa cttcattggc tgttgccgac ttcttctcca aacgtcatga cgatgttctg
     3961 aaaaagatcc gtatcttgga ttgttcgtca gagttttgtg cccgcaattt tgcggagaca
     4021 tcgatttcgc taaatcaacc gaatggtggt acacgcaaac ttccctgcta ccaaataacc
     4081 cgcgacggtt ttgcgtttct tgctatgggc ttcactggta aacgtgcagc ccggtttaaa
     4141 gaggcataca tcaatgcttt taaccagatg gagagaagct tatcaggagc tggcgcggct
     4201 gacatgtcat ctgtcgcaca aaatgccaga ggcgtatacc tgcatttgcg tgaaatccat
     4261 caaatctgga aaagccagct ttatcccatg cttaaggccg ttgaatctcc gctggctagc
     4321 aaactgtacg accgtgtagg tgatgctgtt tttggggccg cacttgttga ttccagactg
     4381 aacggttctg acaaggaggt gcgcccatga ttcgccgcgt cgttaatttc ctgtatcacc
     4441 gatacaaccg ttgcccccgt gtggggcagt ggttcaccac cagcaacgga cacgttctgc
     4501 gggtttgcct ggttaataca gaaagccaga aggttgtctg tcaggttcag gggcgtactc
     4561 ataccctgag ttatccgctg gtggcgtttc agtccggaaa aatgtttaag cgtctgggag
     4621 gtgccgtgtg atttgtaaat taacgcctga aaaaaaagcg ctcaataaga gttcaaggct
     4681 gcttttatct tatttgcaaa atgcttcagg tgagcagggt tttgtggaaa tttctcttct
     4741 ggaattgtcc gcagctcttg gcttatgccg taacacaatc acaaacgcta cggcattact
     4801 cgaagatctt ggcgttgtca ctgtttccag ggggagaagg aataaaacga aaagtggaaa
     4861 ggaaagtgca gggaaaaata tttacaggtt gatccagtca gcgaccaata ataaggacta
     4921 cgccatagtc atgccgcgta aggaggtcgt atgagtcgtt acgctcctac accggaggtt
     4981 atggctattg gtcaaattaa tatttccggc aatgtcacac cgccatcctg gtggaaacat
     5041 atccagctac ccagtgggcg tccagatgcg actgccattg cgctgctttc agagattgtt
     5101 tactggtatc gcccgacaga agtcagggat gagcatacag gggctttact gggatatcgc
     5161 aagcgttttc agagtgacaa gctgcaaaga agctatcagg cgtttgctga gcagtttggc
     5221 tttgggaaaa gggaaaccgc agatgcgctg aagcgtcttc gtgatgccgg gtttattact
     5281 ctggatttac gcacggtgga aatgctcgat ggggtgaaat gcagcaatat tttgtttgtc
     5341 gggatcaacc cacaggcaat tgctgccatc accacacctt cttctgtttc gccagaaagt
     5401 aacagcaata atgcaatcag cgatacagct attacgttaa aacggaacac cccccaacgt
     5461 cataacggaa caggggatac gccgaatgtt gatacaaata cagagattac tacagagatt
     5521 acaacggaga ctaaaaacac tattggcgca tccgctgacg cgtctgcacc agcgcgttct
     5581 gccagacagg aatattcacc ggaatttgag caggcctggc aggaataccc caaacgtgct
     5641 ggtggtaatt ccaagtcagc agctttcaaa gcctggaaag cccgaatcag ggaaggtgtg
     5701 acaccagaaa ccatgctcaa cggtgtgaaa cgctatgccg cctgggtgcg tgtctcagga
     5761 aatatcggca cccagtttgt gaagcaggcg tcgacgttct ttggccccga tagacacttt
     5821 gacgaatcct ggcaacagcc tgccgttccc ggaggtgggc ggcaaagaca ggtcgatgtt
     5881 ctgtctggcc ttggtgccat gtctgacaaa ttcggtaaat ccagcgacaa actgacattc
     5941 tgaggtgacc acgatgatgg cggctaacca gcgtgaaaaa caaacaagac tacaggcgcg
     6001 aatggatgaa ctacggtctg agatggcgtt tgcgaagtca ggggaaaaac catggcctta
     6061 tcgttcatgc ctgatacgag aaggacgtgg atattgcgaa aaacatggcg aatatcacac
     6121 gcatactctt gtgtggggtg atcgtaatgg cgaggacaga gagaaagtct cacgttgccc
     6181 agattgtctg agtgctgaaa taaacgatgt gatgatggag ttgtcgtccc tgaaggcgga
     6241 agaactgact gataacgccg gaattgccct gcgttttcgg gactgcgagt ttgataatta
     6301 tctggaggtt aatcctgacg cagccagaaa tcttgcggcc tgtcgccgtt atgcggagaa
     6361 ctggccggat atgctggaga acggtaccag tcttgttatg accggcagtt gcggcaccgg
     6421 gaaaaatcat ctggcggtat caatgacaaa acatatcatc cgcaactatc tcgccagtgt
     6481 tgagattact gacgtgatgc gtcttacccg cgcggtgaaa aactgctggc ggaatgacag
     6541 tgaaaaaaca gcggatgacg tcattgagca ttatgcgtca ctggatttgc tgattatcga
     6601 cgaagtcggt gttcagtttg gcagtgtggc tgaaatggcc attttgcagg aaatcatcaa
     6661 tgcccgctac gaaagtatct tgcccaccat tctgattagt aacctttcac cggaagagtt
     6721 gtgggcgttc atcagtcccc gaattgccga caggatcacc gatggcgggc gcaactggtt
     6781 gtcgtttaac tgggccagct accgttctcg tatcggaggt gtggccgcat gaccagccag
     6841 aacaccccgg catggcgtaa cgatgacctg gaaggtgcag tgattggcgc gttttttctg
     6901 cgtggggctg atccggaagt gatggatatt ctggccacgc ttccggcgga tgtctttttc
     6961 gtgcgtcagt accgggatat ttacacgggg atctgcagac aggcccgtgt atccggcgtc
     7021 attgaccccg tgctgttgtg caatgagatg ccggagcttg ccccggtgat taccgacacc
     7081 ggtcgtaaaa cctgggtgaa gtcttcactg gagcactatg ttgcagcgct gcggcgaaat
     7141 gccgcactgc gcgatgcaga aaaaacactg accgaagcat tacagaattt acgtgatgcg
     7201 catacctgtg aagcagccga ggatgccctg aaggatgcgc agaacatgat ggccacactg
     7261 tcgacaggaa agggcgtcat tcagccggtt cacattgatg atgtccttcc ggaagtggtc
     7321 aaccgtgttg aattccgcag tcagggactg gagaaatcca gggcgctgat gactggtatt
     7381 gatgaactgg acgcaaaaac gggaggtatg gaaccaggcg acctggtatt cattgcggct
     7441 cgtccttcga tagggaaaac cgaacttgcg ctggacatca tcgacaaggt gactgagcag
     7501 gggcatggcg tgcttctgtt caccatggag atggcgaaca tccagattgg tgaacgtatg
     7561 gtgtctgctg ccgggggaat gccggtatcc cgtcttaaat ctgttgcccg ttttgaagat
     7621 gaagactggg cgcgtttctc tcagggagtg ggacgaatga cggggcgtaa tatctggatg
     7681 gtggaccagg caaacctgac cattgatgag atatgtgcaa ccacgaggca ccaccggatg
     7741 aaacacccgg aaacggcgct ggtggtggtc gattacctcg gcctgattaa aacccgtagt
     7801 accggacgtc acgaccttgc cgtgggggaa atctcaaagg gacttaaaag cctggcaaaa
     7861 tccggcggtt tcccgctgat agcgctgagc cagctctctc gcggcgtgga atccagaccc
     7921 aataaacgcc ccatgaactc ggacctgaaa aactccgggg aaatagaggc ggatgcagac
     7981 atcattctga tgctttacag ggatgaggtg tacaacccgg acacacaggc gagaggcata
     8041 gcagaaatca acatcacgaa acagcgtaat ggcacgctgg gtaccattta ccggcgtttt
     8101 cataacgggc attttctgcc tgtggaccag gagagtgccc gggttctctc cactcccatg
     8161 acgccgggca atccgcgcag atacagcaat aaccgcatgc cgggcagtaa aaatgggcgt
     8221 ttattttgaa caacagaaca atcactgttt caccggaaca acttcgtcgg caggcgcagg
     8281 agatgcttcg ttgtgctgaa cagatggaaa aaacgagcgt ggcaaaagat acgctccgca
     8341 agcagcttac tccggcgctt cgtgatctgc tgcaggcaaa acaccgcaca caaaaggcgg
     8401 tggatgagct ggtggattgc gtggcggaac tggaaggcca ggtaagccag tttgaaacac
     8461 tggtgaagga gtttactgcg tgatggctga atttttttct cctgtgttca tgcaatatcg
     8521 gttgctgagg tgaacgtgag agcactgctg acccctgaaa ttgccccgcg tatggggatc
     8581 gtattgttca gacccggttc agagctgatg cccctgttta tgcaggggcg tgttctgctg
     8641 gagcctgagc cggaacgtta ttcatctttc gccagtggtg ctgttccggc ggcatcacaa
     8701 ccgctggcgg atgatcctgc tgtacgggcc gtgttccgca atgaggcagt gatccgtcgt
     8761 gctggtggcg tggaatgtct tgaaagctgg ttacttcgtg aaaagggctg tcagtgacct
     8821 cattccgact ggcacagcga gaacatgacc acaatgcgac acgctccggg cgcaatccgt
     8881 ctgtgctggc actgcgataa ccagttgcgc gatcagttca cggaacggct ggaatcaatg
     8941 gcaacggata actgtgcccg ctgggtgcta tctgttgtgc gacgtgatct cggttttgat
     9001 gacagtcacg ttgtgacaat gccggaactg tgctggtggc tggttcgtaa tgacctggcg
     9061 gatgccttac cggaaagtgc agcccgtaag gcactgagat taccgaaacc tgttgtgcca
     9121 tctgtcaccc gggaaagtga ccttgtgcct tcggttcctg ccaccagcat catccaggat
     9181 aaagcgaaaa aggtgctggc gctgaaagtg gatccggagt cgccggagtc ttttatgtta
     9241 cgccccaaac gtcgtcgctg ggttaatgaa aagtacacgc gctgggttaa gacacagccg
     9301 tgtgcatgtt gtggtaagcc agctgacgat ccacatcacc tgattggtca tggtcagggg
     9361 ggaatgggta caaaagcgca tgacctcttt gtgttgcctt tgtgcagaaa acaccacgac
     9421 gaactgcatg cggatactgt ggcatttgaa gagaagtatg gctcccaact ggagctgata
     9481 tttcgtttta tcgatcgtgc gctggcgatt ggtgtgctgt cctgattttg tggagaaagt
     9541 taatgcgtga tattcagatg gttcttgagc gctggggagc gtgggcggca aataaccatg
     9601 aggatgtgac ctggtcaccc attgccgccg gattcaaggg gctgatcccc gaaaaagtaa
     9661 aatcacgtcc acagtgcagt gacgatgacg cgatgattat atgcgggtgt atggcccgtc
     9721 tttaccggaa caacagcgat ctgcatgact tgcttgttga ttattacgtc ctcgggatga
     9781 cgcttatgtc actgggccgt aagcatgagc gctctgattg ctgggtcggg cgtgtattgc
     9841 aaaaagcaga aggggttatt gagggaatgt tgatggctct ggatgtgaag ctggagatgg
     9901 atccgtatgt tgagcgtgaa ccgtctggaa catcgaccga tcagtttgcc ggacaaacgg
     9961 aaaattgaaa gcgaatgctt ttattgtaga atggttgcgg gtgcttgagg ctgtctgcct
    10021 cgggcatgaa catcaacggc agatagataa aagccccacc cgactataaa tcgaagtgag
    10081 gcccctatat gctcgtcaca tatagattgc ctcttacgga ccgaaaggtc aaggagaagc
    10141 aggctatgaa gcagcaaaag gtgatgttaa tcgccctgat cgtcatctgt attaccgtcg
    10201 ttgtgacggc gctggtaacg aggaaagacc tctgcgaggt acgaatccga accggccaga
    10261 cggaggtcgc tgtcttcgta gactacgaat ctgagaagta agagacctgg cgagggagaa
    10321 atccctcgcc acctctgatg tgtcaggcat cctcaacgca cccgcacttt attcgtcata
    10381 tgttccgcaa aatgcaggta gtccttgcat ctgtgcggta attttttgtg tgcgtgtact
    10441 taaaaagcgt tgattttcac aaaaggatga ttttatgctg ccagaccagc gaccagcgac
    10501 cagcgaccag cgaccagcag taaatcaaga aagtctgtaa aatctgttga accagatatt
    10561 gccgatcggg ggaacggctg gctaaagtct tatggtcttg actataaact cgagcaggca
    10621 aaacttaaca cagagataga caacgcactg gatgcctatt tttcaaaaaa tggtggtgtt
    10681 ggtggcaacc gaccagatgt aaagctcctg cttaaagata agtatggtaa gcaatacccc
    10741 gttcttattg agtacaaagg ttacaaagac aagctcatta aactgggcgg caatggcatt
    10801 atcgaaaata aagactcaag aaaagaatgg aattttaaaa atattaatgg atacgcggtg
    10861 aatggcgcgg tccattatgc taacgcactc cttcagttca ccagttatcc cgatattata
    10921 gctgttggca tgacaggttg gcgtgatgat gatacgggag aactgcatca tgaaatcggc
    10981 gtatggcacg tatcgaaaaa taatttagga gcggggcaaa aagttggtga gtatgctgat
    11041 ttatcgttcc tttcagcatc aaactttgat gactttatta acaaagtcag cctgcttaat
    11101 ctgtcccctg aagaactgga aaaaattaaa gccagcaagg aagaagagat cgatacgcgt
    11161 ctttctcgtc ttaacaatga tatctatcaa aatgaaaagg gacttggtga aaacgaccgc
    11221 gtttatcttg ttgtggctac ggttattgct actctgggga ttcccggaaa acttaccccg
    11281 ctggataaaa gtgagcttgt gtcttcaacg gaagaaggag aacgtgatgg tgatattatt
    11341 ttcagaaaag ttaaaaattt tctggcgata aaaggcgttc cggaaaccaa aaggggcata
    11401 atccttcgta ctcttcagaa taccctgtgg accgaaaaca tcaataaacc tgtgaatggt
    11461 gaaagtcagt taaaacgggt atttattaaa gttgttgatg acctgggcga atattacaaa
    11521 atcgggctga ctaccgattt caccggcaaa ctctttaacg agatgtaccg ttggctggga
    11581 tttactcagg ataaacttaa tgacgttgtt ctgactccac cttatgtagc tacattgctg
    11641 gcaaaactcg ccagagtgaa taaagactct tacgtgtggg attttgcaac aggttcagcc
    11701 gggttactgg ttgccgcaat gaatgaaatg ctcactgatg ccagagataa aattaaatca
    11761 ccggacgagc ttaaacttaa agaggtacaa ataaaggccg aacagctcct tggtcttgaa
    11821 gtgttatcca gtatttacat gctggcggtt ctgaacatga tcctgatggg ggatggcagc
    11881 gccaatatcc tcaataagga ctcgctggca gattttgacg gcagatatgg gtttgggaaa
    11941 acagatgaaa aatttccggc gacggcgttt gtgctcaatc cgccatattc tgcaaagggt
    12001 aatggtatga tctttgtgca gaaggcgctg tcgatgatgc acagtggtta tgccgctgta
    12061 attattcagg gctctgcggg ttccggaaag gcgaaggaat ataatcagaa gatacttcag
    12121 gagcacaccc tgctggcaag tatcaaaatg cccgctgatt tgtttatcgg gaaatcaagt
    12181 gtccagactt atgtttatgt ttttcatgtg aaacaggcgc ataacccgaa aaccctcgtt
    12241 aagtttatcg acttttcaaa cgacggttat gcacgctcga atcgtaagaa agccagaaat
    12301 aaccttgtgg atgccgatcg tgcaaaagag cgctaccagg aggttgtgga tctggtacat
    12361 ttcgggaaaa actgcctcag tatttttacg gaagatgagt atttcgaagc aacaatagat
    12421 ccagtaaaag gggatgactg gaaccaaaca aagccagtgg ataccagacc aacactgacg
    12481 gatttcaaaa agacggttgg tgattacttg gcgtgggaag tctcgcaatt actgaaaaaa
    12541 caaggtgaaa atgattttgc gggaaagtaa attccccgct cagcgataaa ttaaaaagtg
    12601 ttgagtgggg agagttcagg attggtgatt tgttctatgt gagcactggt tcgcttattt
    12661 cctcagagag attaaaacca ggcaaaatca aacgtgtttc agcaaagagc gatcaaaacg
    12721 gtatcatcgg agagtttgat acggagttta tggatgatgc aaggcattat gagaatttta
    12781 taagcgttaa tttttttgga gatgtttttt atcatcctta ttgtgccagt gtagaaatga
    12841 aggtgcatgt tcttacatta aaacaaagta agtccggctt taatgcaaag tgcggattgt
    12901 atgttgctag tatgcttaat aaagctttaa gagggcgctt taattatggt caacagttat
    12961 cgagttccaa actcagagat ggaga
//
LOCUS       DAZUCC010000142        12968 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZUCC010000142 DAZUCC010000000
VERSION     DAZUCC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.143"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1700
                     /locus_tag="ACHY09_003478"
     CDS             <1..1700
                     /locus_tag="ACHY09_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFS3886406.1"
                     /translation="LPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLK
                     ARSEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRD
                     SWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKALPEADVALQ
                     RPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDRCVSWLPFYH
                     DMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRR
                     VNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCYGLAENALA
                     VSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAD
                     IPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIK
                     DLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLI
                     HALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
     gene            1697..2611
                     /locus_tag="ACHY09_003479"
     CDS             1697..2611
                     /locus_tag="ACHY09_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS3886407.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLIAMADEITLGIFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLNAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAEARCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SANNNRISEDINWFPGISLEHALRNGLF"
     gene            2643..2891
                     /locus_tag="ACHY09_003480"
     CDS             2643..2891
                     /locus_tag="ACHY09_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS3886408.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2891..4063
                     /locus_tag="ACHY09_003481"
     CDS             2891..4063
                     /locus_tag="ACHY09_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS3886409.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTSYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTISTQPELRQKLMDNANNLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4098..5177)
                     /gene="lptG"
                     /locus_tag="ACHY09_003482"
     CDS             complement(4098..5177)
                     /gene="lptG"
                     /locus_tag="ACHY09_003482"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFS3886410.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVDVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETQ
                     ETSDNLPWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5174..6244)
                     /gene="lptF"
                     /locus_tag="ACHY09_003483"
     CDS             complement(5174..6244)
                     /gene="lptF"
                     /locus_tag="ACHY09_003483"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFS3886411.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6274..6834)
                     /locus_tag="ACHY09_003484"
     CDS             complement(6274..6834)
                     /locus_tag="ACHY09_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFS3886412.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLRNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6846..7670)
                     /locus_tag="ACHY09_003485"
     CDS             complement(6846..7670)
                     /locus_tag="ACHY09_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886413.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPV"
     gene            complement(7670..9019)
                     /locus_tag="ACHY09_003486"
     CDS             complement(7670..9019)
                     /locus_tag="ACHY09_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS3886414.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYASNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9065..9823)
                     /locus_tag="ACHY09_003487"
     CDS             complement(9065..9823)
                     /locus_tag="ACHY09_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886415.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9855..10568)
                     /gene="yghS"
                     /locus_tag="ACHY09_003488"
     CDS             complement(9855..10568)
                     /gene="yghS"
                     /locus_tag="ACHY09_003488"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS3886416.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            10742..11434
                     /gene="yghT"
                     /locus_tag="ACHY09_003489"
     CDS             10742..11434
                     /gene="yghT"
                     /locus_tag="ACHY09_003489"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS3886417.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIEKNEVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11483..>12968)
                     /gene="pitB"
                     /locus_tag="ACHY09_003490"
     CDS             complement(11483..>12968)
                     /gene="pitB"
                     /locus_tag="ACHY09_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFS3886418.1"
                     /translation="VGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQ
                     PQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIW
                     NLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLIVSPIV
                     GLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAF
                     SHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELP
                     QKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSVSQRSQ
                     LRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIG
                     TMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVA
                     GTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT         3272 a         3389 c         3222 g         3085 t
ORIGIN      
        1 ccctacctat gcgctatgcc gattttccaa cgctggttga tgctttggac tacgccgctc
       61 tgagtagcgc cggaatgaat ttttatgaca gacgttgcca acttgaagat caactggaat
      121 atcagacgtt aaaagcgcgt tccgaagctg gtgcgaaaag gttgttatcg ctgaacctga
      181 aaaaaggcga tcgcgtggcg ctgattgccg aaacgagtag cgggtttgta gaggcttttt
      241 ttgcctgcca gtatgccggc ttagtcgccg tcccgttggc gattccaatg ggcgtcggtc
      301 agcgggattc ctggagcgcc aaactgcagg gtttactggc aagttgccag cccgcagcca
      361 ttatcactgg tgatgagtgg ttgccactgg tcaatgccgc gacgcataac aacaaccccg
      421 aattacatgt tttaagccac gcctggttta aggcattacc ggaagccgat gttgcgctcc
      481 agcgtccagt tccaaacgat atcgcctacc tccagtacac ctccggcagc acccgttttc
      541 cccgtggcgt cattatcacc catcacgaag tgatggctaa tctacgtgct ataagccacg
      601 atggcattaa attacgccct ggcgaccgct gcgtctcctg gctgcctttc taccatgata
      661 tgggactggt cggctttctc ctgacccccg tcgccacgca gctttcagta gattatttgc
      721 gcactcagga ttttgccatg cgtcctctgc aatggcttaa attgatcagt aaaaatcgcg
      781 gcaccgtttc cgttgcaccg ccgtttggct atgaattgtg ccagcgccgc gtgaatgaaa
      841 aagatctcgc tgaactggat ctttcctgct ggcgcgtcgc tggtattgga gcagaaccga
      901 tctccgcaga acaactccat caattcgctg aatgttttcg tcaggttaac tttgacgata
      961 aaactttcat gccgtgctac ggactggcag aaaatgcgct ggctgtcagc ttctctgatg
     1021 aagcctgcgg ggttgtggtt aacgaagtgg atcgcgacat cctcgaatac cagggtaaag
     1081 ccgtcgcgcc gggtgctgaa acacgcgccg tatcgacttt cgtcaactgc ggtaaagcgt
     1141 tgccggaaca tggcattgaa atccgcaatg aagcagatat acctgtcgcg gaacgtgtgg
     1201 taggccatat ttgcatctcc ggccccagcc tgatgagcgg gtactttggc gaccaggttt
     1261 cgcaagacga gattgccgcg acgggctggt tagacaccgg cgaccttggt tatctgctgg
     1321 acggttatct gtatgtcacc ggacgcatta aagatctgat tattattcgt ggccgtaata
     1381 tctggccgca ggatattgaa tacattgcgg aacaggaacc ggaaattcat tctggcgatg
     1441 cgattgcttt tgttaccgcc caggaaaaaa tcattttgca gatccagtgt cggatcagcg
     1501 acgaagaacg tcgcgggcag cttatccacg cgctggcagc tcggatccaa agcgaatttg
     1561 gcgttaccgc ggatatcgat ctgttgccgc cccacagtat tccccgaaca tcctccggca
     1621 agcctgcccg tgcggaagcg aaaaaacgtt atcagaaggc ttatgctgcc agtcttcatg
     1681 tgcaggaatc cctggcatga atcaaactgt cgcggtgacg ggcgctaccg ggtttatcgg
     1741 taaatatatt attgataacc tgctcgcccg cggctttcac gttcgcgcat taacgcgtac
     1801 tgcccgcgct cacgtcaatg ataatctcat ctgggtgcgc ggttcgctgg aagatacaca
     1861 ttcgcttagc gaactggttg ccggagccag cgcggtggtc cattgcgccg gacaagtgcg
     1921 cggacacaaa gaagagattt ttacccgctg taacgttgac ggcagcctgc gcctgatgca
     1981 agcggcaaaa gagagcggct tttgccaacg ttttctgttt atctcttcgc tggcggcgcg
     2041 ccatcccgaa ctctcctggt acgcaaattc caaacacgtc gccgaacaac gactgatcgc
     2101 aatggctgac gaaattacgc tgggcatttt tcgcccgaca gccgtgtatg gtcccggcga
     2161 taaagagtta aaaccgctgt ttgactggat gctgcgcggc ctgctgccac gacttggtgc
     2221 gccagataca caactctctt tcctgcacgt caccgatttt gcgcaagcag tgggtcagtg
     2281 gttaaacgcc gaaactgtac agacgcaaac ctatgaatta tgcgatggcg tcgctggcgg
     2341 ctatgactgg caacgcgtac gacagcttgc cgccgaagcc cgttgtggtt ctgtgcggat
     2401 ggttggcatt cctctgccgc tactcacctg ccttgcggat atcagtactg cgttgagtcg
     2461 cctggcaggt aaagaaccta tgctgacccg ctcgaaaatt cgtgaattaa cccacgccga
     2521 ctggtcggca aataataacc gtatttctga agatattaat tggtttcccg ggattagcct
     2581 ggaacacgca ttacgcaacg ggctattttg attgtcttgt ttaaaggtgt gttaacgttt
     2641 ctatggtaaa tcgtgaaata gtaatggatt acatcctttc ctgtttacag gatttggtcg
     2701 aaaacggtgt ggaaattaag cccgacagcg atctcgtcaa cgatcttggc ctggagtcga
     2761 tcaaagtgat ggatctctta atgatgctgg aagacagatt cgatatttcc attcctatta
     2821 atattctgct ggatgtcaaa acccctgcgc aattaatgga aactctactt ccatggctgg
     2881 agaataaata atggggctat acgataaata tgcgcgcctt gctggcgagc gcctgcaatt
     2941 tagtgacaac gggttaacac catttggcac ctgtattgat gaagtgtatt ccgccaccga
     3001 aggccggatc ggcaataaaa aagtgattct ggccgggacc aataattatc tcggtttaac
     3061 ctttaatcat gatgctatcc gcgaaggtca ggcagcgtta gcggcccaag gcactggcac
     3121 caccggttct cgtatggcaa acggcagtta tgcgccgcat ctggcgttag agaaagagat
     3181 tgccgagttt ttcaatcgcc ctaccgctat cgttttttca accagttata ccgctaattt
     3241 aggtgtcatc agcgcacttg ccgaccataa cgccgtggtg ttactggatg ccgacagcca
     3301 cgccagtatt tatgacgcct gttctctcgg cggcgcagag attattcgtt tccgccataa
     3361 tgacgtcaac gatctggaac ggcgcatggt ccgcctcggt gaacgggcga aagaggcgat
     3421 tattatcgtt gaaggcatct acagtatgct cggtgacgtt gcgccgctgg cagaaattgt
     3481 tgatatcaaa cgtcgtctcg gtggttattt gattgtcgat gaagcacatt cgtttggcgt
     3541 gttaggcgca acaggccgcg gactggttga agccgttggc gttgaagacg acgtggatat
     3601 tatcgtcggc accttcagta aaagtttggc ttccattggt ggttttgccg tgggttcaga
     3661 agcgatggaa gtgctgcgct acggcagccg cccgtatatt tttaccgcct ctccttctcc
     3721 gtcctgcatt gcgaccgtgc gctcgtcgtt aagaaccatt tccacacaac cagagctgcg
     3781 gcaaaaactg atggataacg ccaacaatct gtatgacggt ctgcaaaaac tgggttatga
     3841 gttaagttca catattagcc cggtagtgcc agtaataatc ggttcgaaag aggaaggttt
     3901 acgcatctgg cgcgagctta tttcgctggg cgtttatgtg aatcttatcc tgccacctgc
     3961 cgcgcctgca ggcatcacgc tgctgcgctg tagcgtaaac gctgcccaca gtcgtgagca
     4021 aatcgatgcc atcattcagg catttgctac tctgaagcag taatgtgagc ccgctgtggt
     4081 gttgcgccac ggcgggttta atgttgtctg tcataccaat acaccaggct caacgccacc
     4141 agcacaaacg ctaccggtaa accgagcgcg gtaacaggtg cgctcaacgc aaataacaat
     4201 cccagattga ccatgatttg atagctgatc caggtgagta agccaacgat gacgcctaca
     4261 gcgagacggc tacccatccc aggcgaacgc ggggcgctaa aggtaaacgg cacagccagc
     4321 aaaatcatcg ccagggtcaa gatgggttgt cccagttttt cccacagtgc aaggcggtat
     4381 tcgctgctgg gttgcccggt ttcctgcaaa taatggatgt aatgattaag ttgacggaca
     4441 gaaaaagtat ttcccggcat cgataactct tcaagattca tactggtgaa ggccgattgc
     4501 cagggaagat tatcagatgt ttcctgcgtt tctttgccat tcagccattt tttatgattc
     4561 acaccatgca ggatccacgt tttgtcgtct tcaatggtag cactgcgcgc ataaatgtag
     4621 gattcgagcg agagatcatc gcggtaatga aatatctcca cgtccaccgg ctggccctgc
     4681 tcgttcagcg atttcaccgt cacaaattca ttaccacgcc tggcccacag catattgccg
     4741 gtaatgtcat cgtcctcccc caacgcagtg gcagtcgatt tgatttgcag cgcctgttgc
     4801 tgcaatggcg acgccaccca ctcatctatc gcgcctaatg aaacagtcca caatatcccg
     4861 gcaaccagcg ccaccagtgc aatacggaag atagaaaacc ccgtgctgcg aatggcggta
     4921 agctcactgt ttttcgataa ctgccccagg ccgacaatcc ctcccaataa ggcgataaac
     4981 ggcgaaagtt cgaccagtgt gcgcggtaag gtcatcagca ccaccagcac cgcctgagtc
     5041 cagcgataac cacccgggct gacatcatcc agttcgttaa tcaggttgaa ggtggtaaaa
     5101 agcggcagca atagccctgc ggcggcggca aaaccgagaa agagatgacg gattaaatag
     5161 cgactgaaaa cattcatcgg gaaaatttct gcagcaaaga gaagtcgcgt gcgaccaaca
     5221 tcagcaggcc cattagcatg agtccgggta ctaaccacaa accaggaatg aggggaatcg
     5281 cgccgttagc caccagcgta cggcagacgt cgccgccata aaaaatggca acaaacagca
     5341 acgtcagtgg caataacgtt gaaaatcgcc cttgccgcgg ttttacccga cttaatgaaa
     5401 tggccagcag cgccatcaac aatgctgtca ggccacggct ttgtcgccat tgcagttcgg
     5461 catgatcggc aggaaaggcg gagcgcgcca gctccgtgac cgattttgct ttgcgtttga
     5521 cgttagggct ttgatccagc ggattcagat gtaattgcag attacggtag atctgttcgt
     5581 tgtcgtcacg cccctgatga tcgagaagat aggcggtccc gttatgcaac ataacggtcg
     5641 gtttctcagg tgatgggtca accacatcaa ccgaacgggc gcggaaaatg cgggttcgat
     5701 tagcagtaga agtataaatc agcgcatcag tcaggcgatt attatcctga tcaaccgtct
     5761 gcgaaaggat cattcgtccg ttatcgttag tgttaaattt ctttgcccgc aactggcgaa
     5821 catccagctc cgactgtgac tgttgctcca gttgataaat ctgcgcataa gcccacggac
     5881 gaccatacat cgacagaagg gtgacaaaaa tacttagcgg tatcgccaga taaagaacgg
     5941 ctttgtacaa gcgtcccgga ctgccccccg ccgcagagat agcggtaatt tccgagtcgg
     6001 tatacatctg ccctagcgtc acgccaactg acacatacag tccaacaggt aacaacatct
     6061 ccagtgcaat cagcactttg tagaaaacga tatccagcac aacatcaaga gctaatgtcc
     6121 catttgccgc ttcggtcaga taacgctgtg cggagtagct ggcgaaaata aagatcagaa
     6181 aaccgacgat aatcagcacc agacggcgcg taccacgcat gatgtagtgt tcaaccagct
     6241 tcatctcact aacgccctca atcctttgaa tttttattca ttgatcagct tctgcgccag
     6301 cgcaatgaca tgatctacct cttccggcgt caggctgccg tcctttgccc actgaatttg
     6361 cccggtttta ttcagcacca gaatggttga actctgctcc tgcaagtgcc aggcaacgcg
     6421 tccctgtcca ttgccgtcaa taacgaattg cgcccatgga tagcggcgtt tatttttttc
     6481 gattttgcca acgacaaaat agccggtacc aaagatggca tcatcagtgt tcacaatggt
     6541 ggtgggctga aaacgatcct gcggaaagtt tgccgcttca actgctttaa ttaacagtga
     6601 gttctttttc ttcgccgact tacgcccggc gatatactga ataatgcgaa cttttcccgc
     6661 caattgggtg ctgttccact tttggtagtg aatttcgtcg tcgctattcc gaagcagctc
     6721 gccgccttcc tggatataaa cgggggtaac cgttttgcca gtgacgaaat tgtgtgcctg
     6781 cacacccggg gctaagagca tgataatcag cgcaataatc aaacgagatg acatcattgc
     6841 tccggttata ccggaaaacg cgaaatcagc agttctaagc gctggcgaat atcgctatcc
     6901 agcgccatcg gtgcatcata cgactggcgg ttttccatat catccgccac catgtcgtcg
     6961 tgtttatccc ccatcaggcc attaatggcg gcgaggaagc gggaaatctg taacgcttct
     7021 ttattttcag gatcaagcga tcttagggtg tcgtaataag aaacctgctg gctgatgatt
     7081 tttcgcacat catgaaattc cctgccggtt agtcgcggtt tggcaattat ctctttcagg
     7141 gtatgcattt gcttttgccg ataggcaata aaaccgctta cgctttctaa ctggctatgg
     7201 cgtaatgaat agttcaccaa cgaccagacc ggagagctta aataaacccg taacaggttg
     7261 ccgtaatacg acacaatatt tttcttaccg ttacgaaagc cgtcctgaaa acgccccaaa
     7321 aaaacggtcg ttttgccgaa taaaaatccg gcctgatgct tctttaaata cagccgttgc
     7381 gcaaaacgca ggtgcttgta gcgcgctcgc atgtatttaa attgtttgcg ctcatcagca
     7441 gtcagctcat cgcctttggc ctgtttgcat attaaacgca gtaactcctg gcgattgacg
     7501 ccattgagcc agaattgcag gcataacgcg taattatcat ggatgatttc ggtggtacaa
     7561 cttagtgcga tagtcggcgg caaactgacc tgtgcatcaa cgacatcatc ttcttcaatc
     7621 gcaacaaata gacgctcgct cgcctctagc gggaaatatt ttctcatcat caggatttct
     7681 ctccggctgc gttataaagc aaggaatggc gctgaaaaaa tgcctttaag gtcggaatta
     7741 gcgaaagcac cacatccaga cattgaccac cgaaataagc aaacgctgct cccagcacgc
     7801 caaacagatg acagaacatg aacagcaata cgatgtagcc gattgaggcg atggtttgcg
     7861 ccacgagaaa agcgcgctgt tttcccgcca tcaatagcaa tgactcctga gggaagccca
     7921 gcatcgagat cacaattgcg cccagcatca cctgaatcag gtcatacgct tcgagatact
     7981 taacgccaaa caccagcgaa atgagcggct tgccgacaat caacaccgcg agcgccacca
     8041 gaataccgat tccgcccgcc agtaaacctg acttcacgcc taacaaccac ggtctggtgg
     8101 tacgcggatc taaacgcatc acctctgggt aaaaactttt gcccagtaaa cccgctggcg
     8161 ttccggcggc gtcgaagaat gtcatggcga ttttaaataa cccggcggca gcgggtccta
     8221 acacgatccc caccaacact gtgctgcacg aattacgcgc cgaccagatg gaatgggcaa
     8281 tgttggttga ccagacaaaa ctccacgcgc ctttgatgtg tcgggcagac tcaaacagat
     8341 tcaatttgaa cgcgttatgg atatttctgc ggcgtaattc acgtgcggca aaccaccagt
     8401 acatggtgcc gccaacaaga ttcgaggcgt accaggcaat aacaaaaccc gcaaaaccaa
     8461 agtcaaaata ccaggcaacg acgctccccg ctgcgcgcag aaaaggtttc gtcgcttgct
     8521 gtacagcaat tagatcgaag cgatctaccg cacgcagaat gccggtcggc gtggaggaag
     8581 ccattgaagg aatgagcgtg caatacagcg ctgccagcca aaagctttgg tcatccagac
     8641 ctaatgaatg ggaaagaaaa gggagtaagg caatgccgcc gacaatcgcc accgcgccgc
     8701 tgacgatatc cagcgagaag gaaaatgaga cgacattgcg gaattgctgc ggattattgt
     8761 tggttaatgc tggtgttccg tactgaacca ccagctgcca tgtctgaaac ttaataaaat
     8821 cgctgatcga cttggcgtac gattgtacaa tcaccagtac gccaaacatg gcgggcgtca
     8881 tccctttacc ggcacacgag agcgccaaca gacccagcaa agcgctcacg acattactgg
     8941 agcctaacca ggcgctattg cgaataatgg tgcgaaacgc gccatctgca aaccaatgtt
     9001 tgatgttaaa acccgccaat tcagcctgac cttatcgttg atagtaaaaa gtatcccgcc
     9061 agccttaagt taaacttcgg cggtcagaaa cgatggcaac cagagaaacc gccttctgtg
     9121 cctgttccag cacttcgctg tagggcgctc tggaatcaat ctcaagaatt tttgtgccgt
     9181 tatagccaat cgtcgacatg acgccgattt tgtcctgcag ctcggcatag tcatggtcag
     9241 gcttgcggga gatggcagta tcaatatcaa tccccaaacg aatgattaat tcaggacggt
     9301 attgcgccat ttgttggtat aaacgccgtt cgcgctgcgc cagaaacatg ctgattttcc
     9361 cggtcgcacg ttcgacgccg atccccggtc cgtcataata aaagcccgaa atttcagcct
     9421 gcgggaagcg atcgctgacc accagaacgc cactttgcgc cagtcgctga accttgcgta
     9481 gattcgccat tcttcgcagc gagaagcagt acataatcac cgccgcccat agcgctggag
     9541 atttggtttt catgctttgg gttttcgagg atttggccgc cagtcgccgt tcgagccaga
     9601 cgccaaccaa cggcaatcgt ttgattttgt cgccgtcttc gcccgagagc agccccagat
     9661 agcgccgctc ggtttgccag tgttgttgca gcgatttcac caggtcggtg gtcagtgtgg
     9721 atttaccggt gccatcacac ccgaccaccg caatcagccc cggaatgtag ttgggctgcg
     9781 gcgcggttgt actattaaca gtttgtgttt gtagtgcgtc cattcgttaa atcatcccct
     9841 atccattttg gtgatcagga aagcgcggcg tgaattgcgc gtagtgacgc ttgcagaatt
     9901 tcatcggcgg gttgccgacc atccagttca aggatctttg cgccattaaa tgtcagttgc
     9961 ggcgtaacgg cgattttttc ctgtaacgcc gccagttggt ggtcaggttt acgcgcaaac
    10021 gcggtttgtt catcaatgcc aagacgaatc aacaatacgg gcaaataaga cgccatccat
    10081 tggtacagct tcagctcgcg ctgccttaac atttttatcc aaccgttacc gcccgtggtt
    10141 tttgccaatt gcgggccatc aaagcgaaaa cccggcactt caacctgcgg gtagcggtcg
    10201 gtgatgagca gatagccttg ctggctttta cacaacattt tgcgaaactt gtacgcccgc
    10261 cagcaggaaa gcagatagat aactagcgca gtaatattgc caggtggtgt ggagggcttt
    10321 tcgtgcacat gtgccgcttt acttcgcaga taacgcccaa aaggtgcgcc aataacaggg
    10381 agctgtgaaa tccattcgcc aattcgcccg gacgattgcc cgagataaat gtgttctgtt
    10441 ggcattgttg ctgccagttc atttaccagg cttgccgtga gggtcgattt acctgaacca
    10501 tcacatccta caatggcaat aacacgcact ggcgtggaat taattattga catattacgt
    10561 tgattcacga aaaacccggc agcaaataat gtattgaata ttacgtggtc cgtgctcaga
    10621 atatccgttc aaccttattg tgacaatggt taattgtgac attaacctga attacgcttt
    10681 cataaaaaca tattaatcaa ataaatattt tggatggata tttaaattta aggatatatt
    10741 catgcagtca ataacacctc cattaattgc cgttattggt agcgatggtt caggcaagtc
    10801 aacggtgtgt gaacatctta ttaccgttgt cgaaaaatat ggtgctgccg aaagagttca
    10861 tttaggaaaa caggccggaa atgtcggtcg tgcagtgaca aaattaccgt tgatgggaaa
    10921 gtctctgcac aagactattg aaaaaaatga agtgaaaacg gcaaaaaaat tgccgggtcc
    10981 ggttccggcc ctggtgatca ccgctttcgt cgcccgccga ctgttacgct ttcgtcatat
    11041 gctcgcctgt cgtcgtcgcg ggctgattgt cttaaccgat cgttatcctc aggaccaaat
    11101 tcctggcgct tacgatggta cggtgttccc acctaacgtt gaaggcggcc ggtttgtctc
    11161 atggctggca agccaggaac gtaaagcatt tcactggatg gcgagccata agcctgatct
    11221 ggtcatcaaa ctcaatgttg accttgaagt tgcctgcgcg cgtaaacccg accataaacg
    11281 ggaatcgctg gcgaggaaga ttgccataac gccacagtta acctttggcg gtgcacaact
    11341 ggttgatatc gacgccaatc agccactgga aaaggtgctg gttgatgcag aaaaagcgat
    11401 tacggatttt atgaccgcgc gtggttatca ctagtcaaaa atggaaatgc ccgatcgcca
    11461 ggaccgggca ttttcaggaa ggttaaatca actgcaatgc tatccagtac aacccaccag
    11521 aaagaaaaat cgccgccggt aaagtaaaca cccacgccat caggatgctg gttaccgttt
    11581 tacgctgtaa cccaccgccg tccaccacca tcgtccctgc taccgcagac gagaggacgt
    11641 gtgttgtgga gacgggcatc ccaatataac tggcaaggcc gatagacact gccgccgtca
    11701 tttgtgccgc catgccttgc gcatacgtca tgccgcgctt accaatcttc tcaccgatgg
    11761 tcatcgctac acgacgccag ccaatcatgg tgccaatgcc gagcgccaga gctactgcca
    11821 tgatgatcca caccggagcg tactcaatgg tgcttaacat atcgctgcgc agttttttca
    11881 gcaggttctg gtcttcttta ctgacgcctg gcagtttcgc cagcttcgcg gaggtatcag
    11941 agatgcacag cataatgcgg cgcagctggc tgcgctgact cacgcttaac ggctcgtaac
    12001 tttccatatt gcctggcagc atcgttttaa cgcgcgcaat cgcatcaaag gtatttgccg
    12061 gatgacagtg aaactccgtt acttgcgtgc catcagtcga tgccgcaggc aatggaggtt
    12121 ccatcgcaat caacttctgc ggcagttcag gatgctgttg caggtagtgt tcgaagttgg
    12181 tgacggcatc gcgggtacgg gtaatttcat agccggacgc attcatattg acgacgaaac
    12241 cagcaggggc aatccccacc agtaccagca tcaccaggcc gatccctttc tgcccgtcgt
    12301 tagcaccgtg cgaaaacgcc acgcccgcag cggaaacaat cagcgcaata cgcgtccaga
    12361 atggtggctt acgtttgcct tttttctttt tgcgatcttc cggaatgcgg tgaatacggt
    12421 cacgcttttt cgtcccgctc cagaaacggc gcagcaggaa aatcagacca cccgcaatga
    12481 ccaggccgac gataggggaa acaatcagcg aggagaaaat tttggtcact tcacgcaggt
    12541 ttaacgcatc catcaccgat gagccggtta acagcgcgtt ggttaaaccg atgccgataa
    12601 tcgcaccaat caaggtgtgc gaactggagg ccggtaaacc aaagaaccac gttcccaggt
    12661 tccagataat cgcggccagc agcatggaaa agaccatcgc caggccgtga gttgacccca
    12721 tattcagcaa caaatcggtt ggtaacatat ggacaatggc ataggcaacg ctaagtccgc
    12781 ctagcaatac gccaaaaaag ttaaaaaatg ccgccatcac cacggcaagc tgtggctgca
    12841 tggctcgagt ataaataacg gttgccaccg cattcgccgt gtcatgaaaa ccattgattg
    12901 cttcgtagaa caacacaaat gccagagcaa gtaataacaa aagccctgtg tatatatcaa
    12961 ggccaaca
//
LOCUS       DAZUCC010000143        12799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZUCC010000143 DAZUCC010000000
VERSION     DAZUCC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.144"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(31..927)
                     /gene="yhaJ"
                     /locus_tag="ACHY09_003491"
     CDS             complement(31..927)
                     /gene="yhaJ"
                     /locus_tag="ACHY09_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFS3886419.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1032..1733
                     /locus_tag="ACHY09_003492"
     CDS             1032..1733
                     /locus_tag="ACHY09_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFS3886420.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1756..1920
                     /gene="yhaL"
                     /locus_tag="ACHY09_003493"
     CDS             1756..1920
                     /gene="yhaL"
                     /locus_tag="ACHY09_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFS3886421.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2052..3362)
                     /gene="cyuA"
                     /locus_tag="ACHY09_003494"
     CDS             complement(2052..3362)
                     /gene="cyuA"
                     /locus_tag="ACHY09_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFS3886422.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYEIISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3390..4721)
                     /locus_tag="ACHY09_003495"
     CDS             complement(3390..4721)
                     /locus_tag="ACHY09_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3886423.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGVLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4996..6360)
                     /gene="tdcG"
                     /locus_tag="ACHY09_003496"
     CDS             complement(4996..6360)
                     /gene="tdcG"
                     /locus_tag="ACHY09_003496"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFS3886424.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6432..6821)
                     /locus_tag="ACHY09_003497"
     CDS             complement(6432..6821)
                     /locus_tag="ACHY09_003497"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFS3886425.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6835..9129)
                     /gene="tdcE"
                     /locus_tag="ACHY09_003498"
     CDS             complement(6835..9129)
                     /gene="tdcE"
                     /locus_tag="ACHY09_003498"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFS3886426.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9163..10371)
                     /gene="tdcD"
                     /locus_tag="ACHY09_003499"
     CDS             complement(9163..10371)
                     /gene="tdcD"
                     /locus_tag="ACHY09_003499"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFS3886427.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSRRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10397..11728)
                     /gene="tdcC"
                     /locus_tag="ACHY09_003500"
     CDS             complement(10397..11728)
                     /gene="tdcC"
                     /locus_tag="ACHY09_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFS3886428.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11750..12739)
                     /gene="tdcB"
                     /locus_tag="ACHY09_003501"
     CDS             complement(11750..12739)
                     /gene="tdcB"
                     /locus_tag="ACHY09_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFS3886429.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3264 a         3511 c         3048 g         2976 t
ORIGIN      
        1 ttcgcccctt tttattaaca ataacaaata ttattttccg ctaaaaagtt tgggaatttc
       61 ccgcagacac caggatttcg cctcccccat actgtcacga cgccaggcca taataatatc
      121 gatctcgctg gtcgattccg ggctgacgac acgcaaccgc ccttccgcga tgtctttttc
      181 gaccatcgga tacggcatcg tcgccacgcc aagccccgcc agtaacgcct gacgtttatc
      241 ttcaatcgtg ctcaccgtta aacgcggctg tttgtccagt agctgtacgg tcaataccgg
      301 gcgctcacga gcggtatccg ccaccgcaat tccacgatat ttcacgcgcg tcacttcaga
      361 taacggttcc ggctcctgat gaatcgggtg atcaggcgcg gcaacgtaga cgttcattaa
      421 cgtatagagc ttgcgcgagt tgatctccga cgaggagcga aaatgcatat ccggcgcgat
      481 aacaatatcc gcccgcccct gctccagccg ttcccatgcc cccgccagca cttcggtgat
      541 gattgccagt tgggtattcg cttttgccgc cagtttgtca attaacggga aaaaggcagg
      601 tgtcggcacc agcgcttcgg tcacaatggt gagatgcgtt tcccaaccgc gcgcgagagc
      661 ttccgcatcg gtagtcagtt tatctgcggc ttccagcaaa acgcgtcctc gctccagcaa
      721 catcctcccg acattggtga atttggtgcg atggcccgag cggtcaaaca gcaccacatc
      781 cagctcttct tccagttttt gcatggtgta gctaagtgcg gaaggcacgc gtcccagctc
      841 atccgccgcc gccgcaaaac tgccccggcg atcgatcgca tccataaccc gtagtgcttc
      901 cagcgttaat gccctttctt tggccatttc gttctcattc aggaaatttg aacataccgg
      961 gcagaatatc tggctaacaa tgtagcgtcc agccccttac cataaaagga agtaaagaga
     1021 ggtcaagaat tatgattact acccgaactg ccaggcagtg tggacaagca gactacggat
     1081 ggttgcaggc ccggtatact ttttcctttg gacactactt cgacccgaaa ttgttaggct
     1141 atgcctccct gcgcgtgctt aaccaggaag tgctggcccc aggtgccgcc tttcagccgc
     1201 gaacctatcc caaagtcgat attttaaatg tgattctgga tggagaagca gagtatcgcg
     1261 acagcgaagg caatcatgtt caggccagcg ctggtgaggc gttgctgctc tctacccagc
     1321 cgggtgtcag ctatagcgaa cacaatctca gcaaagacaa accgttaacg cgaatgcagc
     1381 tttggctgga tgcctgcccg cagcgagaga atccgctgat tcaaaaactg gcgcttaata
     1441 tgggcaagca gcaattaatc gcctcgccag agggcacagt gggtagcctg caactgcgcc
     1501 agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga aagtgcgaat ttccagttgc
     1561 atggaccacg cgcgtatttg caatcgattc acgggaaatt tcatgcgctt acgcatcatg
     1621 aagagaaagc ggcgctaacc tgcggtgatg gggcgtttat tcgtgacgag gctaacatta
     1681 cgctggttgc cgattcccca ctgcgcgctt tgctgataga tttgcctgtc tagttgtttt
     1741 tacagggaga tgatgatgag taaaaaatcg gccaaaaagc gccagccagt gaagcccgtg
     1801 gtggcgaaag aacctgctcg taccgccaaa aattttggct atgaagagat gttgagcgaa
     1861 ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg aggatgaagc taccgcgtaa
     1921 agacaggtgc cggatgcggc gtaaacgcct tatccggcct acaaaaccca acaacatcaa
     1981 caaattgtgc aattcgtagg ccagataaga cgcgccagca tcgcatccgg cacgataccc
     2041 aaaactggcg tttatctggc cttgctcgcc ataatctcga taatctgccg atccgtttgc
     2101 tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg actgctcaac atcatgcgcc
     2161 acgatcccct cattgccggt cacggcggta tcatccagcg ccattaacac cgctttccac
     2221 gcagccgaag cactggtcga aaccttcatc gcgcagctgt tcgacgctcc atcgcaaatc
     2281 atgccgctga catcgccgat catactgctg atcgccatcg aaatgatttc ataacgccca
     2341 tccaccagcc atgccatccc ggcggcggcc cccattgctg cggtcgttgc ggcacacagc
     2401 gcagacaaac gcggtaactg gttatggatg taaattgcgc tcaaatgcga aagcatcagc
     2461 gcacgcgcca gccgttcatc atcggccccg aagtgttctg ctaccaccac cacgggcatt
     2521 gttgcagtga tcccctggtt acccgagccg gagttactca ttgccggaag cgtagcgccg
     2581 cccatacgcg catcggatgc cgcgctggta cgaatcacaa tggatgaaga gagatctttc
     2641 gccagcaaac cgcgtgcaca ctgtttttcc agcgtcgcgc caatatgcag cccccactta
     2701 ccgctcaaac cttcctgcga taacgcacaa tttagcttcg cggaatcgag aataaagcgg
     2761 atcgccgcaa acgggacttc attgacgaac ttcaggatct cagccagcgt cgttcttgaa
     2821 agcaccgtca gcggcgactc ttgctcgccc tctgtcacac acgcctgctg ggtaaacacc
     2881 acaccattgt gcgtctcaat atgcacaatg ttggtatgcc cgccgacgat ggtgacacac
     2941 gcccacttct caccgttcca gactttggcg cgtgagaaga ggatttcatc gcaaggttcc
     3001 tggatcttaa ctgagacttt ccccgccgcc agcagtgctt tggcatcggc aattgcctgc
     3061 gcagttgcgt ctttcagcac ttccagcccg gcgttagcat ttccacctaa cgctcccagc
     3121 gccgccgcaa ttggcagccc caccattccc gtgccgggaa cggtgacgcc cagaccgttc
     3181 ttcatcagat ttggcgaaac ccaggcttct acacgttcaa ccggaccttc cagttctgcc
     3241 gcagcaaccg ccgccgccag cgccagtgaa atcggttcag tacatcccag cgccggtttt
     3301 acttcctcct gaacggcgag gatgtaacgc tgccataacg gatttaaagt cgaatcaaac
     3361 atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg ccaggaacgg agaaacacaa
     3421 agcaacaaac cagtgacgat aatcaggtac agagacatcc ctttgtattt gtgcaatgcc
     3481 ggtactttgt aaaccagcca cgccgggatc aggcacccta ccatgccgaa aatcgggcta
     3541 cagatagagg tgaagctcaa caccggtgcg ttcagtacga tggcgctcca ggccagcaaa
     3601 atggcgaaaa tcatgatgcc gcgctgaacg agattttcgt taatcttctc ggcaggcatc
     3661 ttgcgacgca ggatgttcat tacgatccct tgcgttgctt cgcgaaagcc taaatagacg
     3721 ccaaagaatg cagtcattac agcaaagata ttgagaatga cgctgaccac tttcacccac
     3781 gctgcgccat caccgctaat aaactgtgcg gcaatcgcca acgcggaaat attctgctcg
     3841 taggctttta ccgcttcgtc atgtcccatc gccagcgtga acgacacggc gtagaaaaag
     3901 acggtgacaa acaaaatgcc aaacgcgata ttcattgccc gcaatgcttt atgccgcgcc
     3961 acttcaattg atttttcccg cgagcgataa gagatcacca tcgggcttaa cgtctggata
     4021 aacagaatcg acgtcagggt aaacggcagc gtaataatgg cgtttttcac cagcagcccc
     4081 agcggcggta gcgaaccgac gttatacaga tgccacattc ccaccatcga cacgcccagc
     4141 gccgcgacca ccagcagctt ggtcagcacc atgccggtcg aaattttaaa taacaatttc
     4201 tcgccgcgtg aggagatcgc caccagaatg caaatcagta ccagaccata aaagggactg
     4261 tctgacagca ccccttccgt cacgccgaag gtatgcaggt aggaagcact atcgttggtg
     4321 atggcggtgg aatagacgaa catccaaatc accagcatta cgaaatagag cgcacctaac
     4381 aggatgcccc agtttttacc taaataaccg ctgatgacgc tcgggtaatc tttacactcc
     4441 ggtgattctg ccagcgtatt aataaacaac cgctgaaaca gatacattgc cgggtaacca
     4501 atcaccgatg agagcaaaaa tacccacaat cccatcaaac cgacctgcac cgggagaaaa
     4561 acaatccccg cgccaatcgc catcccgata ctcataatca cccagccggt gtcagtactg
     4621 tcgaatttga tcgcttctcg ccactcgctc tcactcattc cagcacgacc cgccggggtc
     4681 gaagcgtctg caatgacgcc tttattcgat gcaatttcca taatttctcg ctcaatattt
     4741 tgtagggctt attatttttt tccgagccgc atcaaggcga tatgcggtct acgtttttgc
     4801 aggcgagtta ttagaataaa gaaatgatac gcgcgaggtc agagaaaatc ttcacaatca
     4861 agactttgaa ataacggaga tggataagaa ttttctacta aattaatcgc aagagaatat
     4921 tcattctcta tttgcggcag atcacaaaaa aaaggtgcac atttgtgcac ccaaggatga
     4981 aagctgacag caatgtcagc cacagaccac tttaatggcc agtcctccgc gtgacgtttc
     5041 gcggtattta tcgttcatat ctttgccggt ttcatacatc gtctcgatca ctttatcgag
     5101 tgaaacacgc ggtgccgagg tgcggcgcat cgccatccgc gcggcgttta ctgctttcac
     5161 ggcattaatg gcattacgtt caatgcacgg gatttgtacc tgtccggcaa ccggatcgca
     5221 ggtcagccca aggttatgct ccatcgcgat ttccgccgca ttgcatacct gcgccggact
     5281 gccgcccagg agttcagtta accctgccgc cgccattgaa caggccacgc caatctcccc
     5341 ctgacagccg acctccgcac cagagatgga ggcgttcatt ttatacagcg cgccaatagc
     5401 ccccgctgca agaaaatagc gggcaattga ccgctcgttt accggacgac ggaacttatc
     5461 gtaataagcc agcactgccg gaataatgcc gcacgcaccg ttagtcggtg ccgttaccac
     5521 gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg tacatattga tccagtcgat
     5581 gacattcatc ggatcgttag agatgttatc gctggaaacc agttgacgac gcagcgctac
     5641 ggcacgacgc ggcacattga gcggaccagg cagcacgcct tcagtgttca tcccgcgttc
     5701 aataccgtca tgcatcactt gccagatacg ggcaaaccct gcgtcaattt ccgctttact
     5761 gcgcagcgcc agctcgttgt gcatcatcag gccagatata gacaggccgt tgtaatcaca
     5821 cattttcagc agttcacctg ctgagtggaa atcgtaaggt acgggtgttt cgacatcgtg
     5881 cgacaggccg aagtgttctt cttcgacaat aaacccgccg ccgacagagt aataggtttt
     5941 gcttaatagc tcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg
     6001 caacatttcg ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcgcc
     6061 agaagccacc ggcaaccgcc cgctgcgcgt cactaactct ataaatgcag ggatctcatc
     6121 aatgacaaca tcctgcggac tgtttcctgc cagtcccatg atgatggcga catccgtggc
     6181 atggcctttg cccgtcagtg acaacgaccc gtacagatcg accacaatat ggctcgtcgc
     6241 ggttaataag ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc
     6301 cacagtatgc gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat
     6361 atccacaccc tcggattgcc gttcagtgaa gtggagcgga acgaccttgc gaccgtcccg
     6421 ctcacgaggc cttacgcact acgtactgcg atggcttcaa tttccaactt cacatctttc
     6481 ggcaaacgcg cgacctgcac acagctccgg gtcgggtagg tcgcctgatg ttcatcgaag
     6541 aactgcttat agacttcatt gatggtggca aaatcattca gatcggtgat gaacacggtc
     6601 atcttgatga tatcgcccac actcagcccg gcagcaacca cgatcgcttt gacgttttcg
     6661 aggcttaaac gcgcctgatc ttgcacatca gccgggatct caccggtctg tgggcaaacc
     6721 ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa cgccctgaac ataagggccg
     6781 attgcgcctg gggcacgttg cgtttcgata atctttttca tacatcctcc ggcgtcagag
     6841 cgcctgggta aaggtacgtg aaataacatc ctgttgctgt tcacgggtca gtgcgttgaa
     6901 gcgcacggcg tagccagaga cacggattgt caggttagga tatttttccg ggtgctcgat
     6961 ggcatccagc agcatttccc gattcattac gttgacgttg aggtgttgac cgccttcgac
     7021 atccgcttcg tggtggaaat acccatccag caggccgaca aggttggttt tacgtactgg
     7081 atcttctttg cccagcgccg cagggacgat ggagaaggtg tacgagatcc catctttggc
     7141 gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc acggcgcctt tgcgatcacg
     7201 accgtgcatc gggttagcgc ccggcgcgaa cggtgttccg gcgcggcgac cgtccggcgt
     7261 gttcccggtt ttctgaccgt acaccacgtt agaggtgata gtcagaatcg attgagtcgg
     7321 cacagcattg cgataggttg gcagagcttt aattttcttc ataaagcgtt caaccagatc
     7381 gcaggcaatg ctgtctacgc gctcgtcgtt gttgccatac tgcggatatt cgccgtcgat
     7441 ttcgaaatcc accgccaggc cgttttcgtc acggattggt ttcacacgag catatttgat
     7501 ggcagacagg gagtccgttg ccaccgacag gcccgcgatg ccgcacgcca tggtgcgata
     7561 gacatcacga tcgtgtagcg ccatcagcga ggcttcatag ctgtacttgt cgtgcatgta
     7621 gtggatgata ttcagcgcgc tgatgtactg taccgccagc cagtccatga agtggtcgag
     7681 gctatccatc actttgtcgt agtccagcac gtcatccatc aacggagccg ttttcgggcc
     7741 aacctgaatc ttcagcttct cgtccattcc gccgttgatt gcgtacagta gcgttttcgc
     7801 caggttagcg cgcgcaccga agaactgcat ctgcttacca atcaccattg ggctgacgca
     7861 acaggcaatc gcgtaatcgt cgctattgaa gtcagtacgc atcagatcgt cgttttcata
     7921 ctgcaaagag gatgtgacga tagacacctg cgcggcatat tttttgaagg caatcggcaa
     7981 ggcttccgac cacagaatag tcaggttagg ttccggtgca ggccccatag tgtgcagggt
     8041 gtgtaaatag cggaaggagt ttttggtcac cagggttcga ccgtccagcc ccatcccgcc
     8101 gatcacttcc gtcgcccaga tcgggtcgcc ggagaacagc gaatcaaatt ccggcgtacg
     8161 caagaagcgc accatacgga tcttcatgat gaagtggtcg atcagttcct gtgcctgctg
     8221 ctcattgagt acgcctgctt taaagtcgcg ctcaatgtag atatcgagga acgatgccgt
     8281 gcgccccagc gacatcgcgc cgccattttg cgatttcact gccgccaggt aagcgaagta
     8341 gagccactgc accgcttcct gtgcattctg cgccggacgg gagatgtcaa aaccgtattt
     8401 cgccgccatt tcttgaatct gcaacagcgc acgacgatgt tccgccagct cctcacgcag
     8461 acggatcgta gcttcgagat cttcgccttt ttccagacgg gactggagat cggcaaactg
     8521 cagttcgcgt tcacgtacca gataactgat gccgtaaagc gccacgcggc ggtagtcacc
     8581 gatgatacgc ccacgaccat aaccatctgg taaaccggtc agtacaccag atttacggca
     8641 gcgcagcata tccggtgagt aaacatcaaa tacgccctgg ttatgggttt tacgcagatc
     8701 ggtaaagatg tattcaaatt cgctatccat ttcacgacca tacgcgtgga atgaacttct
     8761 aatcatatta atgccgccga acggatgcag cgcacgcttt aatggcgcat ccgtttgcag
     8821 acccactatt ttttccagcg gctggtcaat atatcctgca tcatgagcgg taattgtggt
     8881 ggcaatattg gtatcgaaat ccaatggcgc gtgagtcgca ttttcgatac ggatgccttc
     8941 catcactttt tcccacaatt ccgtggtggc aggcgtcgct tcggcgagaa aagattcatc
     9001 accttcatac ggtgtatagt tatgttgaat aaaatcgcgg acattaattt cgtttttcca
     9061 gtccgtacct ttaaagccaa gccatgcgtc ggcgtacagc ttatcgctgg tatcaatatc
     9121 taccttcatg aaaaataatc tctctacaat acttcaacta aattatgcaa attctgcggg
     9181 cgcgttcact ttgcctaaat gaatggcatc caaagcaatc attttttctt cgttagtcgg
     9241 aattacggcg caaatgacac gcgcattttc actggaaaca attcgctctc caaaagagtt
     9301 agagcgatta ttcatttctg tatcaatctc tacgcctaat acagctaaat gttccatgac
     9361 cagacgacga attaaacttg aattctctcc tattccgccg gtgaatataa ttccatccag
     9421 gcgatgtaat gaagcagcgt gtccggcaat atgacgggca attcgatgaa caaaggtttt
     9481 aattgccaat tgcgcgcgtt cgtgaccttc atgccaggct ttttccagaa cacgtaaatc
     9541 cgaagaaagg ccggaaatac ctaataatcc cgactcttta ttcacgacgc gttccaggtc
     9601 acccaggctc tggttggttt ggctggcgac ccaggacatc gccccgaagt cgacatcacc
     9661 actgcgggta cccatcatca agccttccag cggcgtcatt cccattgagg tatcaacact
     9721 ctgaccgttg cgaactgcgc agattgacgc gccattgcca agatgcgcca caaccaggcc
     9781 ggagtcatct tccgccagat tcagcaaaga atgcgcgcgc cgggaaacat agcggtgcga
     9841 cgtgccgtgg aaaccataac ggcgtacacc taactcttca taatatttcc acggcaggcc
     9901 gtataaataa gcttccggag ccatcgtctg gtggaaactg gtatcaaata ccgctacctg
     9961 agttacgccc ggaaataatt gctgcgccga ttcaatacca cttaaattgg cgtaattatg
    10021 caggggtgcc agtggagaaa cgcgacggat attatcaatg acttcatcgg taataatggc
    10081 ggactcggta aaaatactgc cgccgtgagc gatgcggtgg ccaattaagg ccacactgtc
    10141 atttaaattc cgtttttcca gttcaaatgc aattgccttc aatgcacctt cgtagctgtg
    10201 gtgagccagc ggtgctggct ctcccccatt tacggataag aatgcatttt ccgagttaat
    10261 accgtcggca atacctgaca ttaatacttc acagtcgctg gcattgagta cggaaaactt
    10321 aatcgaagac gaaccacagt taataaccaa aacaaccgga aattcattca tctcttttct
    10381 catcctgagt tacggattaa aacagtttgt atacgatgtt caggatagtc agcagaccaa
    10441 tcacggtaac aaacacgtta tccagacgac cacggtattt cgccagagac ggcgctttac
    10501 gaatggcata catcggcaac aggcatagca gtgatgcgat aatcggtgcg cccatggctt
    10561 caatcaggtc gaggatgttc gggttggcgt aggcaacaac ccaggtggag cccatgatga
    10621 agatcatgct gagagtattc agtttaccca gcgacacttt ggttttgtca cctttataac
    10681 cgaacttcag aatcaggcca ttcaagcctt ccagcgtccc cagatagtga ccgaagaaag
    10741 atttgaagat agccacgagt gcgatgatgg aagccgcata ttccagtgta atcgcgaacg
    10801 ttgttttggt accggtcatg gacgcaaagt ggttagccag ataagaaagc actggaatat
    10861 tctgcgcttt ggcttccgcc atgttggccg gagacagcgt aaacaggcag ctaaaggcaa
    10921 agaacatcac cactgcaacc atcagcatgc tggcacgaga aatgatttgg gaacatttac
    10981 gttcggtgaa gtcgcgaccg aagtctttct catactcttc acgtttagaa accacgaagg
    11041 aagagacgat tggcgagaag ttaaaggaga aaaccatgat ggaaatcccc agccagacag
    11101 tgatcaggat accgtcatga ccggttaacg acagcgaacc gaggtcaacc tggtcgataa
    11161 ctgcagagtt ccagtaaggg atcagcgaca gagaaatcag caccaggctg gcgataaacg
    11221 gccataccag gtagctcatc actttaacca tcagatcctt accaaaccag atgacgaaag
    11281 ccatcagcag caacaggaac agcgccacaa agccgcgatt cagcggtgca aagccgagct
    11341 ggttttccca gaacgtcata aaggtattgg taatagtaac gccataaatc cacagcagtg
    11401 ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc gccagtttta ccaaaatgct
    11461 cttccaccgt ttccgtaatg ttgccggaag ggttagagcc agaaagacac agacgcgcca
    11521 gtgcccggtg gcaataaaac gcgatggggt atgctaatac caacatcaga agaatcggga
    11581 tcagtccgcc aaaacctgcg cggataggga agaacagcac cccggcgccg attgccgtac
    11641 caaacaagcc taacgtccat gtggtatctg atttacgcca ggacgattgt tttgtctggc
    11701 tggatacaat gctatctgaa gtactcatat cctatcctca acgaattaat taagcgtcaa
    11761 cgaaaccggt gatttgagag acgcgagaaa gatcgatatt gccgccggaa ataatactga
    11821 cggtttttct gttttgaata tattggtcta atttaccgct taataatgca gcacatgcca
    11881 gagcgcctgc gccttcggtg acgactttat tgcgctgaat taaggcaatc atactgtttc
    11941 tgatttcgtc ttcgctgacc agcacgatgt catcgactaa ttcacgaacg atttcgtaag
    12001 ttaaattacc cgggcgggag acatcacaac catccgccag ggtgccggta gttcggtgcg
    12061 tagttatttc tccggagtgg aaagaagccg ccatgccgtg aacgttttct gactgtacgc
    12121 caataacacg aatagtcggg ttaattgatt taatcgctac cgcaatacca gcaattaaac
    12181 cgccaccgcc aattggcaca atcacattat cgacatcata gagatcttcc ataatttcca
    12241 gaccaatcgt tccctggcca gcaatcactt tcggatcatc gtaaggtggg ataaaaatac
    12301 ggccttccat ttcgacaatt tcgctcactt tagcaatagt gtcgttgaag ttatcaccat
    12361 gcaaaacgac ttctgcggag tagtcgcacg ttgccgcgac tttggatttc ggcgcacctt
    12421 ttggcatcac cactttaccg tcaataccca gcatcgcaca ggagagcgaa accccttgcg
    12481 catggttgcc cgcagagcag gccactacac ctttacgttt ttccgcatca gtcagtgaac
    12541 ttaatttatt aaatgcgcca cgaattttaa atgaaccggt acgctgcatg ttttcgaatt
    12601 tgaggaatat ttcacctttg caacgttcac taaaatagtt ggagcgaggc atacctgttt
    12661 tataaattcg cccagccagt cgttgttttg cttcaatgat gtcatcaata gcaaccggga
    12721 gatcgtatgt aatatgcatt ataaaacctc ttagcctgaa ttaaatatgt aggtaaccgt
    12781 aaccgacacc tgcaaaaca
//
LOCUS       DAZUCC010000144        12796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZUCC010000144 DAZUCC010000000
VERSION     DAZUCC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.145"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            166..1224
                     /gene="citC"
                     /locus_tag="ACHY09_003502"
     CDS             166..1224
                     /gene="citC"
                     /locus_tag="ACHY09_003502"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFS3886430.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFCHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1239..1535
                     /gene="citD"
                     /locus_tag="ACHY09_003503"
     CDS             1239..1535
                     /gene="citD"
                     /locus_tag="ACHY09_003503"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFS3886431.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1532..2440
                     /gene="citE"
                     /locus_tag="ACHY09_003504"
     CDS             1532..2440
                     /gene="citE"
                     /locus_tag="ACHY09_003504"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFS3886432.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2451..3983
                     /gene="citF"
                     /locus_tag="ACHY09_003505"
     CDS             2451..3983
                     /gene="citF"
                     /locus_tag="ACHY09_003505"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFS3886433.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVYLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3987..4538
                     /gene="citX"
                     /locus_tag="ACHY09_003506"
     CDS             3987..4538
                     /gene="citX"
                     /locus_tag="ACHY09_003506"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFS3886434.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKLGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4513..5391
                     /gene="citG"
                     /locus_tag="ACHY09_003507"
     CDS             4513..5391
                     /gene="citG"
                     /locus_tag="ACHY09_003507"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFS3886435.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5442..6905
                     /gene="citT"
                     /locus_tag="ACHY09_003508"
     CDS             5442..6905
                     /gene="citT"
                     /locus_tag="ACHY09_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFS3886436.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7019..7824
                     /gene="rna"
                     /locus_tag="ACHY09_003509"
                     /pseudo
     CDS             7019..7824
                     /gene="rna"
                     /locus_tag="ACHY09_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
     gene            8054..8464
                     /gene="rnk"
                     /locus_tag="ACHY09_003510"
     CDS             8054..8464
                     /gene="rnk"
                     /locus_tag="ACHY09_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFS3886437.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8607..8696
                     /gene="yldA"
                     /locus_tag="ACHY09_003511"
     CDS             8607..8696
                     /gene="yldA"
                     /locus_tag="ACHY09_003511"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFS3886438.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            8821..9249
                     /gene="uspG"
                     /locus_tag="ACHY09_003512"
     CDS             8821..9249
                     /gene="uspG"
                     /locus_tag="ACHY09_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFS3886439.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9370..10935)
                     /gene="ahpF"
                     /locus_tag="ACHY09_003513"
     CDS             complement(9370..10935)
                     /gene="ahpF"
                     /locus_tag="ACHY09_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS3886440.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11106..11669)
                     /gene="ahpC"
                     /locus_tag="ACHY09_003514"
     CDS             complement(11106..11669)
                     /gene="ahpC"
                     /locus_tag="ACHY09_003514"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS3886441.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12041..12787
                     /gene="dsbG"
                     /locus_tag="ACHY09_003515"
     CDS             12041..12787
                     /gene="dsbG"
                     /locus_tag="ACHY09_003515"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFS3886442.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKMLNIIMGNK"
BASE COUNT         3019 a         3369 c         3245 g         3163 t
ORIGIN      
        1 atttcacgct gcaatactcc ttaggatgta tagcgaaagg agaaaaagat atacctcgat
       61 cgcccccctt tctcccaagt gaaaataaaa ggttatcagt ttgcaacatc gaacaacatt
      121 tgttgcaaat cgataacaac atgcaccttc aggatactat ttattatgtt cggcaatgat
      181 attttcaccc gtgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg
      241 catgaaaatg atttgagcgt tgacaccaca gtcgaagtat ttattaccgt aacccgcgat
      301 gaaaagctta tcgcgtgcgg tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt
      361 gaatccgttc gcggtgaagg actggcgctg acattagcca ccgagctaat taacctcgcc
      421 tatgagcggc acagcacgca tctatttatt tataccaaaa ccgaatacga ggcgctgttc
      481 cgccagtgcg gtttttccac gctgaccagc gtacccggcg tgatggtgct gatggaaaac
      541 agcgccacgc gactgaaacg ctatgccgaa tcgctgaaaa aattttgtca tccagggaac
      601 aagattggct gcattgtgat gaacgccaat ccctttacga atggtcaccg ttatctgatt
      661 caacaggctg cggcacagtg cgactggttg catctgtttt tagtcaaaga agattcttca
      721 cgcttcccct atgaagaccg actggatctg gtgttaaaag gcaccgccga tattccacgc
      781 ctgactgtgc atcgcggctc cgaatacatc atctcccgcg ctacgttccc ttgctacttc
      841 attaaagaac agagcgtcat taaccattgt tacaccgaaa ttgatctgaa gattttccgt
      901 cagtacctcg ctcccgcgct gggtgtaact caccgctttg tcggtactga acccttttgt
      961 cgcgttactg cccagtacaa ccaggatatg cgctactggc tggaaacacc gactatctcc
     1021 gcaccgccca tcgaactggt tgaaattgag cggctgcgtt accaggagat gccgatatcc
     1081 gcttcccggg tacgtcaact gctggcgaaa aacgatctca cggctatcgc gccgctggtc
     1141 cctgcagtca cgctgcatta tttgcagaac ctgcttgagc actcccgcca ggacgcggca
     1201 gctcgtcaaa agacccccgc atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg
     1261 ttgcaggcac ccttgagtct ggggatgtga tgatacgcat cgccccactc gatacgcagg
     1321 atatcgacct gcaaatcaac agcagcgttg agaaacagtt tggcgatgca attcgcacca
     1381 ccattctgga cgttctcgcc cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca
     1441 aaggcgcact ggactgcatt ttacgtgcac gactggaagc cctgctggca cgcgccagcg
     1501 gtatcccggc tctgccatgg gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa
     1561 aactcgcacc cgccgcagta tgttgtttgt gcctggtgct aatgccgcga tggtcagcaa
     1621 ctcctttatc tacccggctg atgccctgat gtttgacctc gaagactccg tagcattgcg
     1681 tgaaaaagac accgcccgcc gcatggttta ccacgcgctg caacatccgc tttatcgcga
     1741 tattgaaacc attgtgcgtg taaacgcgct ggattccgaa tggggtgtta acgacctgga
     1801 agctgtcgtt cgcggtggtg cggacgttgt gcgtctgccg aaaaccgata ccgctcagga
     1861 tgttctggat atcgaaaaag agatcctgcg tatcgaaaaa gcctgtggtc gtgaacccgg
     1921 cagtaccggc ctgctggcgg cgattgaatc tccgctgggc attacccgcg cggtagaaat
     1981 cgctcacgct tccgagcgtt tgatcggtat cgccctcggt gcagaagact atgtgcgcaa
     2041 cctgcgtaca gaacgctccc cggaaggaac tgaactgctg ttcgctcgct gctccatttt
     2101 gcaggccgcg cgctctgcgg gtattcaggc gttcgatacc gtctattccg acgctaacaa
     2161 cgaagccgga ttcctgcaag aagccgccca catcaaacag ctgggctttg acggcaaatc
     2221 gctgatcaac ccgcgtcaga ttgatctgct gcacaacctc tacgcaccga cccagaaaga
     2281 agtggatcac gcccgccgcg tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg
     2341 cgtggtttcc ctgaacggca agatggtgga cggtccggtt atcgatcgcg cccgtctggt
     2401 gctctcccgt gcagaacttt ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga
     2461 aaattgaaca atctcaacga caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg
     2521 atcttgccgc tttccagaac tcgccaaagc aaacctacca ggctgaaaaa gcgcgcgatc
     2581 gcaaactgtg cgccaacctg gaagaagcga ttcgtcgctc tggtttacag gacggcatga
     2641 cggtttcctt ccatcacgct ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg
     2701 tcatcgcgaa gatgggcttt aaaaacctga ctctggcctc cagctccctg agtgattgcc
     2761 atgcgccgct ggtagaacac attcgccagg gcgtggttac ccgcatttat acctctggcc
     2821 tgcgcggtcc actggcggaa gagatctccc gtggtctgct ggcggaaccg gtgcaaatcc
     2881 actctcacgg cggacgtgtg catctggtac agagcggcga actgaatatc gatgtggctt
     2941 tcctcggcgt cccgtcctgt gatgaattcg gtaatgccaa cggctactcc ggtaaagcct
     3001 gctgcggctc cctcggctat gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc
     3061 ttaccgaaga actgctgcct tatccgcata atccggcaag cattgagcaa gatcaggttg
     3121 atctgatcgt caaagttgat cgcgttggcg atgctgcaaa aatcggcgct ggtgctaccc
     3181 gtatgaccac taacccgcgc gaactgctta ttgcccgtag cgctgcggat gtgattgtta
     3241 actctggcta cttcaaagaa ggtttctcca tgcagaccgg caccggcggc gcttcgctgg
     3301 cggtaacccg tttcctggaa gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc
     3361 tcggcggtat taccgcaacg atggtttatc tgcacgaaaa aggtttgatc cgcaaactgc
     3421 tggatgtgca gagctttgac agccatgctg cgcaatcgct ggcccgtaac cccaatcaca
     3481 tcgaaatcag cgccaaccag tacgctaact gggggtcgaa aggcgcagcg gttgatcgtc
     3541 tcgacgtggt ggtactgagc gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga
     3601 ccggctctga cggcgtactg cgtggtgctt ccggtggtca ctgcgatacc gcgattgctt
     3661 ctgcgctttc catcatcgtc gcgccgctgg tacgcggtcg tattccgacg ctggtggata
     3721 acgtgctgac ctgcatcacc ccgggctcca gtgtcgatat tctggtcaca gaccacggca
     3781 tcgctgttaa cccggcacgt ccggagctgg cagaacgtct gcaggaagcg ggcattaaag
     3841 tggtttccat tgagtggctg cgtgaacgtg cgcgtctgct gaccggtgaa ccacagccga
     3901 ttgaattcac cgaccgcgtc gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg
     3961 ttgtgcatca ggtgaaggaa taagccatgc acctgcttcc tgaactcgcc agccaccatg
     4021 cggtgtcaat tcccgagctg ctcgtcagcc gggatgaaag gcaagcacgg caacacgtct
     4081 ggctcaagcg ccatcctgtt ccactggtct cctttaccgt ggttgcgcct gggccgatta
     4141 aagacagcga ggtcacacgc cgaattttta atcatggcgt gacagccttg cgtgccttag
     4201 ccgcaaaact gggctggcaa attcaggagc aggctgcact ggtttccgcc agcgggccgg
     4261 agggcatgtt gagcattgcc gccccggctc gcgacctcaa gctcgccacc attgagcttg
     4321 aacatagtca tcctctcggg cggttatggg atatcgatgt cctgacaccc gaaggcgaaa
     4381 ttctctcccg ccgcgactat tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg
     4441 cagctgtttg cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg
     4501 cgctgctgaa cgatgtcgat gcctgcaacg tcaactaaaa acacaaagct tgcgacgtca
     4561 ttaatcgatg agtacgccct gctgggctgg cgcgccatgc tgactgaagt caatctgtca
     4621 ccgaaaccag gcctcgtgga tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa
     4681 gatttccacc gcagcgcgct ggcgattcag ggctggttac cccgtttcat tgaatttggt
     4741 gcctgtagtg cagaaatggc accggaagcg gtactcaacg gattacgccc aattggtatg
     4801 gcttgcgaag gtgatatgtt ccgcgccact gcgggcgtaa acacgcataa aggaagcatt
     4861 ttttctttag ggctactgtg tgcggcaatt ggtcgtttgc ttcagctcaa ccagccggta
     4921 acgccaacaa ccgtttgttc tacggcggca agtttctgcc gtggcctgac cgatcgcgaa
     4981 ctgcgtacca ataattcacg actgacggca ggtcaacggt tgtaccagca gcttggcctt
     5041 accggcgcac gcggtgaagc cgaagcgggt tatccactgg tgatcaacca cgccttgccg
     5101 cattacctca ctctgctgga tcaggggtta gatcctgagc tggcattgct cgataccttg
     5161 ctcctactga tggctaccaa cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc
     5221 ctgcgctggc tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc
     5281 gccgatctcg attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca
     5341 ggcggcagtg ccgacctgct gatccttacc tggtttttag cacagattta attatttaag
     5401 cacttgataa atttggaaat attaattttc ggagaacccg tatgtcttta gcaaaagata
     5461 atatatggaa attattggcg cccctggtgg tgatgggtgt catgtttctt atccctgtac
     5521 ctgacggtat gccgccgcag gcgtggcatt actttgcagt gtttgtggca atgattgtcg
     5581 gcatgatcct cgagccaatt ccggcaacgg cgatcagttt tattgcggtt actatttgcg
     5641 ttattggcag taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg
     5701 cgcaaaaaca ggcgctgaaa tggggtctgg ctggtttttc cagcaccacg gtatggctgg
     5761 tatttggcgc atttattttt gcattagggt atgaagtttc cgggttaggc cgtcgtattg
     5821 cccttttcct ggtgaaattc atgggcaaac gcacgctgac gttgggttat gcgattgtca
     5881 ttatcgacat tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg
     5941 tttttccggt cattaaaaac ctgccgccgt tgtttaaatc attcccgaac gatccgtccg
     6001 cgcgtcgtat tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt
     6061 ccatgtttgt caccggtgcg gcaccaaacg tgctgggtct ggagttcgtc agcaaaattg
     6121 ccggtatcca gattagctgg ttgcagtggt tcctctgctt cctgccggtt ggggttatct
     6181 tgcttatcat tgcgccgtgg ctttcctacg tgctgtacaa accggaaatc acacacagtg
     6241 aagaagtggc aacctgggcg ggtgatgaac taaaaaccat gggtgcgctg acacgcagag
     6301 agtggacgct gattggcctt gtattgctca gcttaggttt gtgggtattt ggcagtgaag
     6361 tcattaatgc tactgcggtt ggtctgctgg cagtttcgct aatgctggcc ctgcacgttg
     6421 tgccgtggaa agacattacc cgctataaca gcgcatggaa cacgctggtc aacctggcaa
     6481 ctctggttgt gatggctaac ggcctgactc gttctggttt tattgactgg ttcgccaata
     6541 ccatgagtac gcacctggaa ggattctcac caaacgcaac ggtgattgta ctggttctgg
     6601 tgttctactt tgcacactac ctgtttgcca gcctgtctgc gcacaccgca accatgctgc
     6661 cggttattct ggccgtcggt aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc
     6721 tgctggtgct atctatcggt atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg
     6781 tgattattta cggctgtggc tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct
     6841 tcggggtgat ttacatctct atgttgctgt tggttggctg gccgattctc gccatgtgga
     6901 actaatacgt tcgccaaacg ctgaataaat catcacgccc gccaggtaaa cccacctggc
     6961 gggctttttt atgattaaat agataatatt cagtgtatgt atgagttcca cacccattat
     7021 gaaagcattc tggcgtaacg ccgcgttgct cgcggtttct ctgcttccct tctcttctgc
     7081 caacgccgtg gcgttgcagg caaaacagta tggcgatttt gatcgctatg tcctggccct
     7141 ctcctggcaa accggatttt gccagagtca acacgatcga aatcgtaacg aacgagatga
     7201 atgtcgcctg caaaccgaaa cgaccaacaa agctgatttt ctgaccgtac atggtctgtg
     7261 ccaggattgc ctaaatcggt tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc
     7321 ggttgcgcta ctcgcccaat cccgaatcta ccagaagcgc gcgccagccg aatgtgttca
     7381 tcgccggaaa ccgggttatc actggaaacg gccactaaac taagtgaagt catgccagga
     7441 gctggcggac gttcctgcct ggaacgctac gaatatgcca aacacggtgc ctgctttggt
     7501 tttgatccag acgcatactt cggcacgatg gtacgcctga atcaagaaat taaagaaagc
     7561 gaagccggaa aattccttgc ggataactac ggtaaaacag tgagccgccg tgactttgat
     7621 gccgcctttg ccaaaagctg gggaaaagag aacgtgaaag cagttaagct aacgtgccag
     7681 ggtaaccctg cgtatttgac tgaaattcag atctcggtca aagctgacgc catcaacgct
     7741 ccgctttctg ccaactcatt cttgccacaa ccacacccag gcaactgtgg caaaaccttt
     7801 gtgattgata aagcaggtta ttaatcctct gatttacaaa acacgctaat tcgtcttcca
     7861 taaaccatga cgattaagat ttatacataa tgcgctacat tgaggaattc cctgaatgtg
     7921 acgcaaatca cttcaaagag tgagcaacag tcagtatcat catgtgagtt aaaccctcgc
     7981 cgcctgacgg tgagggtttt cttttgggta tgtttatctg cgacactcgc agtaccgacg
     8041 acatggagta aaaatgtcca gaccaactat catcattaac gacctggatg ccgaacgcat
     8101 cgatattctg ctggagcaac ccgcctatgc tggtttgcca atcgccgacg cgttaaacgc
     8161 agagttggat cgcgcccaaa tgtgttcgcc agaagagatg ccacacgacg tggtgacaat
     8221 gaacagccgg gttaaattcc gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt
     8281 gtatccggca aaaatgaccg acagcaatac tcagctttcc gttatggctc cggtaggtgc
     8341 cgcactgctg gggctgcgcg ttggcgattc cattcactgg gaacttccgg gcggcgttgc
     8401 aacccacctt gaagtgctgg aactcgaata ccagccagaa gctgctggcg actacctgct
     8461 ttaatcccat ctgcacaagc gataactcgc tttgcccaga ggatgcactc gcatcctctt
     8521 tccgctcaaa tatcaaccag atttatcatt ttgctcttaa gcgcagcatt ctaatctgaa
     8581 ctggctattc atctgcggaa cctcatatga gcgaagctct cggtatcact gcattttata
     8641 tattcatagc agcaattatt gttgctgcag tgctatacct tgaacaacgc tggtagcgcc
     8701 tggtttttat ttattttcca gctcaatagc aaattcagat caaatcagcc tcgcaaatta
     8761 tagattcgta accaataatg tgctgttatg tatacatgca aaaacaagga gagaatgctt
     8821 atgtacaaaa caatcatcat gccagtggat gttttcgaaa tggaattgag cgacaaagcg
     8881 attcgccacg ctgaattcct cgcccaggat gacggagtta ttcatctact tcacgtacta
     8941 ccggggtcag ccagcctgag cctgcaccgt tttgccgctg atgtacgtcg ttttgaagag
     9001 catctgcaac atgaagcaga agaacgtctg caaacgatgg tcagccactt caccatcgat
     9061 ccttcccgca ttaagcaaca tgtccgtttt ggtagtgtgc gggatgaagt taacgagtta
     9121 gcgaaagaac ttgatgcaga tgtagtagtg attggttcgc gtaacccatc aatttcaact
     9181 catctgttgg gttctaacgc ctcgagcgta atccgccacg ccaatttgcc ggtgctggtt
     9241 gtgcgttaat tgccagaata agtctcccgc cctgcccgga gccatccctg cagggctttt
     9301 taatgcaata aaaaagccgc caggtttgac cccggcggct aagcaattac aggtgaatct
     9361 tacttcttct tatgcagttt tggtgcgaat caggtagtca aaagcactca gagaggcttt
     9421 ggcaccttcg ccagtggcga tgatgatctg cttgtacgga accgtcgtac agtcacccgc
     9481 tgcgaacacg cctttgacgt tggtttcgca tttcgcatca atgataatct cgcccatgcg
     9541 attacgttcg actgcgcctt ccagccagtt ggtgttcggc agcagaccaa tctgcacgaa
     9601 aataccggcc agttcgatgt tgtgaatatc gccgctgaca cgatctcgat attccagacc
     9661 tacgactttg ctaccgtcgc ctttcacttc cgtggtttgc gcattcagaa taatgtcgac
     9721 gtttttcagg ctgcgcagtt tgtcctgcag aacctggtcg gctttcattt ctggcgcaaa
     9781 ttccagcagc gttacgtgct caacgatacc cgccaggtca attgccgctt ccacgccgga
     9841 gttaccgccg ccgataaccg ctacgcgttt acctttaaac agcgggccgt cgcagtgcgg
     9901 gcagtaggtc acgcctttgg tgcgatactg atcttcgccc ggaacgttca tgttgcgcca
     9961 ttttgcaccg gtcgccacga taatgctgcg tgctttcagt accgcgccag aagctgtttc
    10021 aatctgatgc aggccacctt caaccgctgc cgggatcagt ttgctggcgc tctggctgtc
    10081 gatcacatca acgtcgtatt catcaacgtg aactttcagc gctcctgcca gtttctggcc
    10141 ttcggtcttc ggtacagaaa tgtagttttc gatatcaacg gtatcgagga tctgaccacc
    10201 aaaacgttcg cccatcagac cggtacggat gcctttacgt gcggagtaaa ttgctgccgc
    10261 tgcacccgcc gggccggaac cgacgattaa tacgtcataa gcatcacgct tgttcagctc
    10321 ttctgccgca cgtttttccg caccagtatc aattttggca acgatttcag tcaacgtcat
    10381 gcggccctga ccaaactctt tcccgtttac gaacactgcc ggaacgccca tcacgttgcg
    10441 atcggtgatt tcgttctgga aggtgccgcc gtcaattgca gtgtgcttga tgcgcgggtt
    10501 cagtacgctc atcaggttca gcgcctgcac cacgtccggg cagttgtggc aagagagcga
    10561 gtaataggtt tcgaattcaa aatcaccgtc aatatggcga atctgctcca gcagagactg
    10621 cgcttctttc gacggatgac caccggtcca cagcaacgcc agtaccagcg aggtgaactc
    10681 gtggcccagc ggggagcctg caaaacgtgg cccctggtta gaacctgggt tggtgatcag
    10741 gaaagacggc ttacgcaccg gcaagctgtt atcttcttta aaggtgactt tgtctgacag
    10801 ttctgcgatt tcagccaaca gttccttgat ttctgccgat ttagcgctgt catccagcgt
    10861 ggcaattaac tcaacaggct tggtcaattt ctcaaggtaa gccttgagtt gagttttcat
    10921 atttgtgtcg agcatgttta tctcctgggc tttaaacatc atcatgcaag cggcctctgc
    10981 ggctacctgg atgcagcttg catcatggtg cggggaagaa aatcgggtgc cgcagcaccc
    11041 gaagaattag gtgaatttcc gaagtatttc gcgccggagt tagtagcgcg aaagacaaag
    11101 gaaatttaga ttttaccaac caggtccaga gacggagcca gagttgcttc accttctttc
    11161 catttagccg ggcaaacttc acctgggtga gaagctacgt actgtgctgc tttgatttta
    11221 cgcagcaggt cagacgcgtc acggccaatg ccttcagcgg taacttcgat tgcctggatg
    11281 ataccctgcg ggtcaacaac gaaggtcgca cggtcagcca gaccttcatc ttcacgcatg
    11341 ttgtcgaagt tacgggtcag ggcgccagtc gggtcgccga tcatcgcata tttgatttta
    11401 gcgatggttt cagagctgct gtgccatgct ttgtgggtga agtgagtatc ggtagatact
    11461 gcgtatacgt ctacgcccag tttctgcaat tcttcgtaat ggtcagcaac gtcacccagt
    11521 tcggtcgggc atacgaaagt aaagtcagcc gggtagaaga agaagacgct ccagcggcct
    11581 tcggtatctt tttcggtgat ttcgatgaat tcgccgtttt tgaatgcctg gtttttaaaa
    11641 ggtttaattt tggtgttaat caaggacatc tatacttcct ccgtgttttc gatgagatgt
    11701 aaggtaacca atttttgccg attcggctaa tgcgtttcca ttatcaaatc gataagtttt
    11761 accttacaac cttcgtaaga caactttgtg aactttgcac cgccagcggc gataaaaagt
    11821 aaaatggccc cctttccagg tgaccgtgta atctgaacta ccctttggta tcttcagcgc
    11881 ctgcctgcag gctgaggtgt tgcccttaca aatgcaacaa cgacatggat tacaacaccc
    11941 tcataaacaa agggcaatca cctgatctaa gctcttacct atgacagtga taggttatgc
    12001 cttttactcg acttttgcac tgactgaaaa ggacaaatta atgttaaaaa agatactttt
    12061 actggctctg cttcctgcaa tcgccttcgc agaggaactt cctgccccag taaaagcgat
    12121 tgaaaaacag ggtattacaa tcatcaaaac gttcgatgcc cctggaggaa tgaaaggtta
    12181 tctcggtaag tatcaggata tgggcgtcac catctacctg actccagatg gtaagcacgc
    12241 tatctctggt tacatgtaca acgaaaaagg tgaaaacctg agtaacacgc ttatcgaaaa
    12301 agaaatttac gcacccgccg gacgtgaaat gtggcaacgg atggaacaat cccactggct
    12361 cctcgacggt aaaaaagatg cgccggtcat tgtctacgtc ttcgccgatc cgttctgccc
    12421 atattgtaaa caattctggc agcaggctcg tccgtgggta gattctggca aagtgcaatt
    12481 aagaacattg ttggttgggg ttatcaagcc agaaagcccg gcgacagcag cggcaattct
    12541 tgcctccaaa gatcccgcaa aaacctggca acaatatgaa gcctctggtg gcaagcttaa
    12601 gctaagcgtg cctgcaaacg taagcacaga gcaaatgaaa gtgttaagcg acaacgagaa
    12661 actcatggac gatctggggg caaatgtcac accagctatc tattacatga gtaaggaaaa
    12721 taccctgcaa caagccgtcg gattacccga tcagaaaatg cttaatatca ttatggggaa
    12781 taaataaaag ataatt
//
LOCUS       DAZUCC010000145        12775 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZUCC010000145 DAZUCC010000000
VERSION     DAZUCC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.146"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            695..2185
                     /locus_tag="ACHY09_003516"
     CDS             695..2185
                     /locus_tag="ACHY09_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFS3886443.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGESLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(2307..2879)
                     /locus_tag="ACHY09_003517"
     CDS             complement(2307..2879)
                     /locus_tag="ACHY09_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886444.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2965..3171)
                     /locus_tag="ACHY09_003518"
     CDS             complement(2965..3171)
                     /locus_tag="ACHY09_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3886445.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            4028..4252
                     /gene="hha"
                     /locus_tag="ACHY09_003519"
     CDS             4028..4252
                     /gene="hha"
                     /locus_tag="ACHY09_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFS3886446.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(4584..4781)
                     /locus_tag="ACHY09_003520"
     CDS             complement(4584..4781)
                     /locus_tag="ACHY09_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886447.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            4980..5549
                     /locus_tag="ACHY09_003521"
     CDS             4980..5549
                     /locus_tag="ACHY09_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFS3886448.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            5810..6211
                     /locus_tag="ACHY09_003522"
     CDS             5810..6211
                     /locus_tag="ACHY09_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFS3886449.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            6199..6633
                     /locus_tag="ACHY09_003523"
     CDS             6199..6633
                     /locus_tag="ACHY09_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS3886450.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            7408..7634
                     /locus_tag="ACHY09_003524"
                     /pseudo
     CDS             7408..7634
                     /locus_tag="ACHY09_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(7636..8418)
                     /locus_tag="ACHY09_003525"
     CDS             complement(7636..8418)
                     /locus_tag="ACHY09_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFS3886451.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            8721..9641
                     /gene="rbsK"
                     /locus_tag="ACHY09_003526"
     CDS             8721..9641
                     /gene="rbsK"
                     /locus_tag="ACHY09_003526"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS3886452.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            9669..10985
                     /gene="fucP"
                     /locus_tag="ACHY09_003527"
     CDS             9669..10985
                     /gene="fucP"
                     /locus_tag="ACHY09_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFS3886453.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            10997..12010
                     /locus_tag="ACHY09_003528"
     CDS             10997..12010
                     /locus_tag="ACHY09_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS3886454.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVKGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTTQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            12432..12716
                     /locus_tag="ACHY09_003529"
     CDS             12432..12716
                     /locus_tag="ACHY09_003529"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3886455.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
BASE COUNT         3295 a         2824 c         3211 g         3445 t
ORIGIN      
        1 atctgctgag atattattag ttggtggtgt ttttactgag taatagctgg cttttaattc
       61 ctgaactgaa atgctgtcgc tcgtcagggt ttaattaaaa ctggatgatg atgtgaaaat
      121 gaaggctggt ttttgtgttt gtcgaggaag tgttgttttt atccattctc atcattggct
      181 ggctgcgatt atacagaagt atttgaagaa aaaatggtgc attttatctg tgccggcagg
      241 gaggaggatt ttcctgatat gtcagtatta aatggttttt gttatttata aaaatgtcat
      301 cctgtccata gaatattgta ctgagtactc agcattcagc gcaggaactg tcatcttctt
      361 tattttattc ttcttcagga gggcagtttt ctgaaagcgt tactccgggg aaactgttta
      421 tgtttattcc cggcaggaaa cccgtacctg caataccctg gatgatgttt tttgctggta
      481 cgttgttgtg tgaaaattaa tcaacatgtg tgctccggag agcgcccgta gacgcacaga
      541 ttcattctga cattctttag cgacaaaata ccgggtggtt attatttaac ctgaaaatcg
      601 acagaaattt ttatcctgta ttgattctgc tgctattttt gctattagta gcaatagttg
      661 cggtacttta tgttctgtgt gttgtgaggt cattatgcca tactttcagt atcctgatga
      721 gtttcccctg agctcgctgc ctccactcat tcgtgatgcc gttattgaag ctcagcaaat
      781 cacgcaggct cccctggggc ttgtggcagc atctgccctg ggcgctgttt cacttgtctg
      841 ccagaatctg attgatgtct gcagactgaa tacactgcgg gggccggttt ctcttttctt
      901 actgacgctt gctgagtccg gagaacgaaa aaccgcagtg gacaaattat taatggagcc
      961 cctgtaccag caggagatgc tgttatattc cagacacaaa aatgaactga cgacatggaa
     1021 aaataaggaa gagcttttaa aggcgcagaa aaaagcattg ttgtcaaaac tgaataaaga
     1081 actccgcaaa ggcgcggatg agtcagaaac gctcagacaa ctggaagcgc ttcagaaaaa
     1141 cagaggggaa aaacctgtaa gatataaatt catttttaat gatgccacga cagcagcaat
     1201 taaagatcag ctttgtggtc agtggcgttc agtgggtatc atgtctgatg aagccggtat
     1261 aatatttgat gggtataccc tgtctgagct gccttttatt aacaaaatgt gggatggctc
     1321 cgtattgtcg gtggacagaa agaatgaacc cgaacagatg attgaaaatg ccagaatgac
     1381 attatccctc atggtacaac ccggtctttt tgaccgatat atggagcgaa aaggttctgt
     1441 tgccagggac agtggttttc ttgcccgttg tctaatcagt aaacctgcca ccacgcaggg
     1501 gaaacgcttt attaacggag cagtgacccc gggagaatca ttaacggcat ttcatgaacg
     1561 attaatggaa ctggccagag gcagtattga aaaatccagt aaggatgaac gttattgtct
     1621 gcatttttct ccagaagcgc aaaaaatatt tattgatcac tataatgttc tggagcagga
     1681 tttgtctcct tccggaccct taagccagtt tcgggggcat gtttcaaaaa aaacagaaaa
     1741 cattgcgaga attgctgcgt tattgcagta tttcagtcag ggtgaaggga aaatctctgc
     1801 cgatatcatg acgtctgctg tcgtaataag ttcgtggtac acagatgaat ataaaaaatt
     1861 atttgcgtta cctgatgaat ctgaactgca gctgcaggac gcccgggagc tgctcgactg
     1921 gctcattgag gagtgccgcg gagaatgccc gccccgtgtc aggaaaaatt atattctcca
     1981 gtgtggcccg ggccggttca ggaacaggaa aaagctgaac gtcctgctga acattcttgc
     2041 atctcaactc aggctctctg tcgtgcagga aggaaaaacc acctatgtcc tgctttccga
     2101 aataaacaat atcacgctgc ctgaactgaa tgcgtgctac agccagaaac tgataagatg
     2161 gcatgagcaa tcctttggct tataatgttc cctgttgctg agacggatta ttcagtggtg
     2221 gtatcgcagt atgcccgtca ccccccgctt ctgcatgagg gtgacggagg aaaagtaagt
     2281 gtgcggaggc ccgggagcgt tatgaatcaa caggcgtata acaggtactc gtcaatcatc
     2341 agttccagct cgcgcttttc ccgtttgcgc gccacagccc agttttcaag ctctgcatgt
     2401 gatttatgct ttaactcttc agtccagggc tgcatggagt gggcacacat cagatcatac
     2461 cagcacagcc atatcagcgt gtggtagaga tcacgatcgt cgaaacgccg gagcaggctg
     2521 ttcagcctgt ttgccagccg gttttcaccc agacgatgta gctcactgag taaaacgccg
     2581 cccagattaa aatgtgtaag cagcatgatt tcatgggtct gaatgctggt cattaaccct
     2641 tcctgaatgg caggaatgaa cggtggtgtt cccgggttga cgacctgata gagtccctgt
     2701 ggtgccactg agatgatagt gcctttttcc ggcagaaaaa agtgggccag ggtcaggcga
     2761 tgataaccat gaagatgcgg agaccccatt ccatgagcga cgataagcct cgtcacgctt
     2821 gtcatccatt tctgatgctg gatgagcagc agctcacacc ggttttcaag tttttgcata
     2881 atattctctc ctttagcgcg cagcaaaatg cctccggggg ccggatcatg cctttgctgc
     2941 ggttgaacgg ataacggtca ggaattacgg acgggactgt gcaatacgcg cctgcagcca
     3001 ggtttccact tcacttttca gccatcggga gctacggccc agtttgatgg gggcaggaaa
     3061 ggcaccatcc ttgatgagct tgtaaaacca tttatcggtc aggccggtca gttgagtgat
     3121 aaatgccatg tcgaccatct ggtcatccat cagtgaaact ggggtattca tgagaatgtt
     3181 ccttcggatg ttgttaatct caaccggcgt tggtgatgaa aacaacgcac caccttcacc
     3241 ggcataacaa ccggggagaa acgtaaaaaa taatcgggaa tgtgaccgtc aggagataaa
     3301 cggggggatt gcggtggctg agccgtaccg gtatgtcggg tgattgttgc gaaacgatgc
     3361 ttccgcacgc gcacgggagg actttcgtga gacaagggga aaactggcgt tctgataatt
     3421 gcagagttgt ttttgaaggc ggttgtggtg catcagcctg tgacctgtgg gcaggcgaca
     3481 ggtatcactg gtgggataag actgtctgat tggtattata aatatataag agagctcaaa
     3541 cacatgagtt catatatgtg aaataaatct gtgagtgtga tcagcagacc agtctaccca
     3601 gtagcaacag attgcgttgt actcacaggg ttatccctga taactggcgt ataacctgcg
     3661 tacataaaac gtacctgcaa tgcacctgaa cagagtgcat taagcatccg cctgagcggt
     3721 aaacgtatcc gatctggtga gtgacagtct gagagcattc tcagtaatcg cccccttgta
     3781 tcctggaaat tggttaagag cgattagatt tggcaaggtg tatgcatcca gcagcttaac
     3841 acctcagcta actggctggt taccttcagg ttgtcgcaat ggcatcggac atccggtgtc
     3901 ggataactaa acgtagcgtt aaggcgatat tgctgagaga ccggttaagt ggtggcacgg
     3961 gagtaatgag tttggggagg gtaccagaat gccagtcagg tgcggtatgc tggcagtatc
     4021 acgttgattg cagcataaag taacgagaga cgaatggaag ttaaaaccag agcagttccg
     4081 tcgttgttca tcccgggaga cgctggaaaa agtgattacc cgcacacgtt ataaacttac
     4141 ccctgcggag ctggaagcct ttaactctgc ggtcgatcac cggctggcag aactgacaat
     4201 gaacaaactt tacgatcgcg tgccggcttc cgtctggaaa tatgtcacct gaatcttgaa
     4261 tccgcgctac gatgactctg gtgttatctg tgcaccggaa gatcgggaac attaagcagt
     4321 ataacggcaa caaagaattc gaaatcaaaa gggaatggtg tacagagggg caggcgttca
     4381 ggcattaccg gcctgtatct tctgaagggg actcgtattt gtcggggctg agtattccgc
     4441 tcgattcgat cacggattgg cagaaacctg actgtgattg attcaggtac cattttgcag
     4501 ggatccggtg cgttactgtc tggcacaaca aacgcagcgg gtcgctctgc cccgccgatt
     4561 cctgataatt cctcccctgt aacctactgc aacagtacaa atacactgac gcctgaaacg
     4621 gtaaccagaa tcagcaccag cagtaacgtc agtaaacaga gcagacgatc ggtcttccgg
     4681 taataaccga aggggtcgac agcgccacaa tacgggcaac acactgtatt atgagtaatt
     4741 gcgtttccac agtccctgca cttctttttg cgtatcacga tcgctccctc tccacgcctt
     4801 atccgtaacc tgttttttac attaaaaaaa caacgccggg aattcaatcg tcagtccgga
     4861 gacggccgtt cgggttatca cagagtgcct gagacagtgt cctgccggag gtcacctcag
     4921 gggctctgcg cattttttac gacgtggcta ttccgtatga accatacgga gatttaacca
     4981 tgacctacaa atacaacccc ttctggcagc aacgtattcg tgagacggtg cggcacgcac
     5041 tggatgttca tccccgcctg acggcacttc gggttgatct gcgtctcccg gatgtgccgg
     5101 cagcaacgga cgcggctgtg atatcccgct tcatcaatgc cctgaaagcc cgaatcgacg
     5161 cttatcagaa acgtaagcat cgggaaggta aacgcgtgca ttcctcgacc ctgcattacg
     5221 cctgggcccg ggagttcggg gagcttaaag gcaaaaaaca ctatcacctg ctgctgctgg
     5281 tcaaccggga tacctggtgt cgtgccggcg attaccgcgc tccggggtca ctggccggga
     5341 tgattaaaca ggcctggtgc agtgccctgg gagtggatgc cggacgttat gacacgctgg
     5401 cacattttcc tgacaagcca gcggtgtggc tggaacgcga tgatgaaacc ggctttcagc
     5461 aggtgctgga acgttctggc tatctggcga aggagcatac caaagctcgc ggcaccggtg
     5521 agcgcaattt tggctgtagc cggagttgaa cacgacaggc gcaccaatac ccggcaaacc
     5581 aaaagtatgg gttgttgtct ggcggcaacg atgtatccac tcgcccactg actgtttttt
     5641 acgtcagtgg gccattgtgt gacaaccaca gtgctgtaac agcaaccagc ctgctttacc
     5701 caaactccag tccagatttt aactgaaccg ccattcacac cctgaccacg ctgcccgtac
     5761 cgggacggct gttgcctgcg tgtcgtctgg cggctgagga acatatacta tgtacgcaaa
     5821 atcctttctc gctcttgatg gcaacggacg tctgacgggg gcccgtaccg cacagactgc
     5881 gccttacgac cgctacacct gccacctttg cggcagtgca ctcaggtacc acccgcaata
     5941 cgacactgaa cgtccctggt ttgaacacac tgacgacggg ctgactaagc acggtcatga
     6001 gtgcccttat gtcagaccgg aacgcaggga ggttcgcttg attaagcgtc tgcagcaatt
     6061 cgtaccggat gccttacccg tggtgcgtaa agccagctgg cactgcagac aatgtcacca
     6121 cgattattat ggggagcagt actgcacaaa ctgccagacc ggaggattca gtattccccg
     6181 gacaactcag gaggaaatat gcgaattctg aactgttata tggcgaatga cagcaaaggc
     6241 cattttgtta cggcgaaaga agctgcgaag cacaaccgac aggacgtttt gtgctgtgtg
     6301 tcctgtggat gcccgttaac acttcagcgg ggcaatgacg gacaaccacc gtggtttgaa
     6361 catgaccaga tgactgtcgc tgaaaaaatc ctgctgcgat gtacctggct tgacccggca
     6421 gagaaagagg cccgtcgttt gcatctgcag ggcatgacgg ttccggatta tacggtgaag
     6481 gtgagaaagt ggttttgtgt gatgtgtgac gaagattatg agggggaaaa gtgctgccca
     6541 cgctgcggta ctggggtata cagcagggcg tgggggcggc aggaggtgcc gtcggaagat
     6601 gccagggctg ataatccgtt acagaggctg taatggttgc ctccggagca gtttgcggta
     6661 atgccgttcc cgggaatgga ttgccagtgc ggggactgtg gagcttaatt ccggtgttgg
     6721 tggcctttcc gttgattttg agccaacagc cctcctgtga ctgacaggct gcctcgtcat
     6781 tccattcgta tctccagtaa cagggggttg tattgtattt taatgtgccg gttcaggcgt
     6841 gcgtttcccg gcgcgtctgc accggcttaa ccaaattcaa cagggataaa atagtgtaaa
     6901 taaccgaagt attacttgca gaatcggggg aagcatccgg ggttttattc aacaggcatg
     6961 ggaggctccg gctctgacag acattgtact gtggaatgtt gttacaggtt ggggcggcaa
     7021 ccgtgccagg agaccatgag ccgttcacgg cattaaaggc cacacttcgg tcgtttacat
     7081 tccctgtggt ggcaggcatc ttccgttaag gcgtcagagc gttaccaacg ttatgttgtc
     7141 atggctcacc tttcatagtc gatgcttatc tggtgactga aactagccgc agataaagag
     7201 tgtgaagttg ataccatgga gtggagtgac gtgcaggcag gagattcgct ctatgcaggg
     7261 ttgtgagatg aattcaaacc ggctgtcagc gccagtaata tttgaagatt cgtccggttg
     7321 ttatccagtg tgcattaaaa acccagacat tatggacaat acaacggctt ccataacagg
     7381 gaggagcctg tttattatcc gggcgtaatg actaaccttt gtgggctttt tcccgtcgtg
     7441 cttttaacgt tgaaggcatt ttctgcctgc cgattcagga cagcgacaaa agccatatca
     7501 gacaattggt caatgatgac cagcgtctgg agtagatgat aagccaaatt gataaactgg
     7561 aatatgtgat gaaagtgcaa cgtaatcagt ccaatccgac gatgtttaac aagagtacgg
     7621 tgttttttca gtaagtcatt cgtcttctga atcaggtgta atgatcttta cagaagcatt
     7681 ccggcagtgc gtaacataat ctgccggtaa cggacgctcg ctaatcagaa catcaaatgc
     7741 agagagcggt gcgaggctgg cagaggctac ttcatcgaac aaggcgtggc gggcgagcaa
     7801 aattttcctt agtccacggg ccatcgcttt acgcttagtg gcaagatctt cgggattaaa
     7861 ccagctgacg ccaaagtgat catgtacacc gctggcggaa ataaatattt ttcgcggatt
     7921 gagagagtct aatggcgaag agttactggc atcgtaaaaa gcatcacttc tggcacgata
     7981 cgtaccacca caaagtattg ctgtcacatt aggtttttca ttcaacgcaa caaagacacg
     8041 atgtgagtaa cagatgccag tgaaggtgat tgcatccggg atcatgctta taacgagcgg
     8101 tatctcctgg ccattatcaa agaagatcag atcattttca ttaaccattc cggcggccag
     8161 aattgcaata ggtaagtcat cacgatgatt tctcggaacg tcctggatta ctggcatgga
     8221 tggtgcgggt ttatgcacca ttacaatata gccacccagt agggtcagtg gcagaggttc
     8281 atcttcctga tggagatcgc gacgaatagt cattacagaa acttccagca ttcgtgccgc
     8341 gtctttcaaa tggattctgt cggttttctt aagtatttca atcaaacgtc ggatacgctc
     8401 tttttgcttc gtttccatca actctctccg taaaatgtta taaaaataac attatggcat
     8461 tagtaaacat tgttttagtt accatactat acgaccgacc ctttgaaatg aaaagggtat
     8521 agactgctga attttcggtg ttaatcctgt taggttgctt atgattagat agtgttggcc
     8581 tgagtgataa gactggaagt ctattaaata aacatcgggt tgatgtgcta atcgcgatct
     8641 cagtcacaaa tgatgcaaaa gtaacatgct aatgttattt aaataccatt gttatgcgaa
     8701 agtttataag aggtagtgaa atggacattg cagttattgg ttccaatatg gtggatctca
     8761 ttacttacac taatcagatg cctaaagaag gagaaactct ggaagcacct gccttcaaaa
     8821 tcggatgtgg tgggaaaggt gctaatcaag cggtggcggc ggctaaattg aattcaaaag
     8881 tcctgatgct gactaaagta ggtgatgata tttttgcgga taatactatt cgtaatctcg
     8941 aatcatgggg aattaatacc acttatgttg agaaagtccc ctgtaccagc agtggtgttg
     9001 caccgatctt tgttaatgcc aactcgagca atagtatatt aataattaag ggagcgaata
     9061 aatttctttc cccggaagat atcgatcgag ctgcagaaga tctgaaaaaa tgcaaattga
     9121 tcgttttaca actggaagtt caactggaaa cagtttacca cgcgattgag tttggtaaga
     9181 aaaatggtat tgaagtgtta ttaaatccgg ctccggcatt gcgtgagtta gatatgtctt
     9241 atgcctgcaa atgtgatttc tttatcccga atgaaacaga actggaaata ttaaccggta
     9301 tgtcggttga tacttatgat catattcgtt tggccgcacg ttcgttagtt gataaagggc
     9361 tgaacaatat tattgttacc atgagcgaaa aaggcgcatt atggatgacc cgcgatcagg
     9421 aagtccatgt tcctgcattc aaagtaaatg ctgttgatac tagtggcgct ggtgacgcat
     9481 ttattggttg cttttcccat tattatgttc aaagcggtga tgttgaagcc gctttgaaaa
     9541 aagccgctct ctttgccgct ttcagcgtca cagggaaagg tacgcaatca tcttatccga
     9601 gtattgaaca atttaatgaa tttttaacct taaacgaata atcatcctgc acagttaaag
     9661 gtagcactat gaacgataaa aacatcattc agatgcctga tgggtatctg aataagactc
     9721 ccttgttcca gtttattttg ttatcgtgtt tattcccgct atggggatgc gcagctgcat
     9781 taaatgacat attaatcact caattcaaaa gtgtattttc gctaagtaac tttgcttcag
     9841 cattggtgca gagcgcattt tatggaggtt attttttaat tgcaatacca gcatcccttg
     9901 tgattaaaaa gaccagttat aaggtggcaa ttttaattgg cctgacgctg tatattggtg
     9961 gttgtacact ttttttccct gcctcgcata tggcaaccta cactatgttc cttgcagcta
    10021 tttttgcgat tgcgattggc ctgagtttcc ttgaaactgc agcgaacaca tacagttcaa
    10081 tgatcggtcc aaaagcctat gccacattgc ggctcaatat cagccagacc ttttatccga
    10141 ttggtgctgc gtctggtatt ttattgggca aatatctggt tttctccgag ggggaaagcc
    10201 ttgaaaagca gatgtcagga atgaacgccg ggcaaattca caactttaag gtgctgatgt
    10261 tagagaacac ccttgaaccg tataagtata tgattatgat tctggttgtg gtcatggtgt
    10321 tgttcctttt aacccgcttc ccaacctgta aagtggcaca gacaagtcac cataaacgcc
    10381 cgtcagcaat ggatacgctg cgctatctgg ctagaaatcc gcgctttcgt cgcggtattg
    10441 tggcgcagtt cctttatgtg ggtatgcagg tcgcagtatg gtcatttacc atccgtctgg
    10501 cgctggagtt gggcgatatc aacgaacgtg atgcttcaaa cttcatggtc tatagctttg
    10561 cctgcttctt tattggcaag tttatagcca atatccttat gacgcgtttt aatccggaaa
    10621 aagtattgat tctctattcc gttatagggg cgctgttcct ggcatatgtt gcactggctc
    10681 caagtttcag cgcggtctat gtcgccgtcc tggtaagtgt tttgtttggc ccttgctggg
    10741 cgacgatcta tgcaggaaca ctggatacag ttgataacga acatacagaa atggcaggag
    10801 cagtcattgt tatggctatt gtcggtgccg cggtggttcc ggcaattcag ggatatattg
    10861 cagatatgtt ccactcacta cagctttcat tcctggtttc tatgctctgc tttgtctatg
    10921 tcggagtcta cttctggcgt gaaagcaaag tacgtaccgc actggctgaa gtcacagcgt
    10981 cctgaggaga accgttatga gtacgcgtat taatttgtgg cgggcgcttt ttggcgagaa
    11041 acctcgtatc ttgctggaaa atagtgattt tactgtcacg tcttttcgat atgacagcgg
    11101 tgtcgagggg cttaaaattg caaacagtcg tggacatttg attattcttc cctggatggg
    11161 gcagatgatt tgggatgcgc aatttgatgg acacggcctc accatgtgca atatgtttcg
    11221 ccagcctaaa ccggcgacag aggtgataga gacctatggc tgttttgctt ttcattcagg
    11281 tttgctggct aatggctgcc catctgcgga agatactcat ctgctacacg gtgaaatggc
    11341 ctgtgccgcg atggatgaag catggatgga attagaggga gatatgttac gccttaccgg
    11401 gcgttacgag tatgtaaagg ggttcgggca tcattatctg gctcagccaa cagtggtcct
    11461 tcacaaatcc tctacactgt tcgatattaa gatggccgta accaatctgg cttctgtaga
    11521 tatgccacta caatatatgt gccacatgaa ttatgcatat ataccaaatg ctacgttcag
    11581 tcagaatata ccggacgaaa tattaaggtt gcgtgaatca gtaccttcgc atgttaatcc
    11641 caccactcag tggctggcgt ttaatcaacg gattatgcag ggagaagcct ctctgtcgac
    11701 gctgaatcaa ccggagtttt atgatccaga aattgtcttc tttgcagaca aactggacgc
    11761 ttacacagat cagccagagt ttcgcatgat cgctcccgat ggcactacgt ttgttactcg
    11821 tttctccagt gccgaactaa attatgtgac acgctggatc ctttataacg gagagcaaca
    11881 ggtagccgcc tttgcattac ctgcaacctg tcggcctgag gggtatcttg ccgcgcaacg
    11941 taatggcacg ctgattcagg ttgcaccgca gcaaacgcgc acttttacag tcaccacagg
    12001 aattgaataa ccactcaagg cctgaacagc ggcgcgctta tcgttaaggt aagcgcgttt
    12061 ttttacccgc caggacaaga ctatgatagc gttgccctta tagacgatca cattatcgtc
    12121 cactccaaat ttgcgcaact actgtagagt ggtgtactcc acagcttctt tgaggagctt
    12181 aacctccatt gttttcttac cgagcaagca ctgcaactct tttatttatt tgtagtggtc
    12241 aacaaaaact ggtcacaatt tcagtttttt tccagtatct attttccgat tcgttcggcg
    12301 gtaatccacc gttatattca tgaggccgga gtgcactata aaagtgtatg cgtaaaccga
    12361 ctgctgtatg tagccattaa gccagtattg gtaacatagg tacccacctt ttcaactagt
    12421 ggacacctgt tatggaacaa aaaacattat cagctgaacc ccgcaggtca ttttcaaatg
    12481 agtttaaact tcaaatggtt aaactggctt tacaaccaag gggttctgtt gcccgtattg
    12541 cccgggagca tgatatcaat gataatcttc tgttcaaatg gctcaggctc tcgctgatca
    12601 aagggcgtat atcacggcgt cttccggtaa cgaactcttc tggcattggc gttgaattat
    12661 tacctgtgga gatgacgctg gatgagcggc cttaccccgt tttttacgcg aggtaattat
    12721 gaagatcaat gactagccgt cgaaccgtgt gcatgaactg ctgcactgaa acttc
//
LOCUS       DAZUCC010000146        12627 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZUCC010000146 DAZUCC010000000
VERSION     DAZUCC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.147"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(17..478)
                     /locus_tag="ACHY09_003530"
     CDS             complement(17..478)
                     /locus_tag="ACHY09_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFS3886456.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQDYGRWSEKIMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQEEARQQKRGLWADSAPVPPWVWRSRK"
     gene            complement(1220..1423)
                     /locus_tag="ACHY09_003531"
     CDS             complement(1220..1423)
                     /locus_tag="ACHY09_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886457.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            complement(1577..2134)
                     /locus_tag="ACHY09_003532"
     CDS             complement(1577..2134)
                     /locus_tag="ACHY09_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFS3886458.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            complement(2237..3097)
                     /locus_tag="ACHY09_003533"
     CDS             complement(2237..3097)
                     /locus_tag="ACHY09_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3886459.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(3156..3902)
                     /gene="traX"
                     /locus_tag="ACHY09_003534"
     CDS             complement(3156..3902)
                     /gene="traX"
                     /locus_tag="ACHY09_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFS3886460.1"
                     /translation="MTTDNTNTTRNDSLAARTGTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNLSRHANIRQSAINRLWGWAVIAQF
                     AYYVAGFPWYEGNILFAFAVAAQVLTWCETRSLWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALLACLLAVIPALNLASSDVAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWPGDFFPVFYACHLTVLGVLAV"
     gene            complement(3922..9192)
                     /gene="traI"
                     /locus_tag="ACHY09_003535"
     CDS             complement(3922..9192)
                     /gene="traI"
                     /locus_tag="ACHY09_003535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFS3886461.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPEAGVIERARAGIDEAISREQLVPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMTFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGHDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLESAISHQ
                     KSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGLERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVLEPGADREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAEEKPDQPDGKTEQAVRAIAGQEQDRASSSERETALPESVLRESQRVQDAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            9274..9501
                     /gene="vapB"
                     /locus_tag="ACHY09_003536"
     CDS             9274..9501
                     /gene="vapB"
                     /locus_tag="ACHY09_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFS3886462.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ESWDEWFDGNSVSDDFMDNREQPGMQERESF"
     gene            9501..9899
                     /gene="vapC"
                     /locus_tag="ACHY09_003537"
     CDS             9501..9899
                     /gene="vapC"
                     /locus_tag="ACHY09_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HFS3886463.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDATAATHTGQIRAELARQGRPVEPFDQMIA
                     GHARSRGLIIVTNNTREFERVDGLRIEDWS"
     gene            complement(9908..12088)
                     /gene="traD"
                     /locus_tag="ACHY09_003538"
     CDS             complement(9908..12088)
                     /gene="traD"
                     /locus_tag="ACHY09_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFS3886464.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEITGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVV
                     DMAVYEAWQREQNPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(12144..>12627)
                     /locus_tag="ACHY09_003539"
     CDS             complement(12144..>12627)
                     /locus_tag="ACHY09_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886465.1"
                     /translation="ISFPEKSGSNPLKPEEGRCNLLSLCVLAPVFCTPKKLAEVSRQI
                     IVTSAKEIAWTPGSSRIDGEQEENITWSLSSQTHADKYDLINKTSRVSPDSGHFYEPG
                     TALDYRERVRLFVQIQYSHESDWSEILRLTDIVLEKVSSGRPAGGYHHRYTGYTWFVL
                     "
BASE COUNT         2759 a         3822 c         3062 g         2984 t
ORIGIN      
        1 agccacagaa atatcattat ttccgtgagc gccataccca gggcggaaca ggagcgctgt
       61 ctgcccacag accacgtttc tgctgtcgtg cttcttcctg caggacggga agaacaggat
      121 cggtgttgta ctgctcataa acccatgcag cgcctgcacg gaccataaac tgattaacat
      181 tcataccgtc tggtgcataa acctgcccga gaatacgacc ataacgatct cgctgaaaat
      241 atgtgacagt aaccgttttt cccgcaacca gagatttcat tattttttca gaccaccggc
      301 catagtcctg tttcttttca ggtgcatcaa tattaacaag acggacacgc accggatgct
      361 gagataacat gacgtcaatg gtatcgccat ccagaacacg cacaacacgt ccatgaatat
      421 cagcagaaaa tacctgacat gaaaaaacaa ataataccac tggtatatat ttcttcattt
      481 tactctccgt cactgattac ttttcgtgat tgcatgaatt ccatgccggg atgaataacg
      541 gctcacgcat cacgccaaaa ataaaccgtt gaattgtgat caattcgaca aaataagatg
      601 aggtggctta atacacatat ggctatgtgt tactaaaaac gctggtcacc ccgcctgacc
      661 agacacataa gaaagaaagt ctcatgcatt attcagcctt ctgatacctg gagtaacgga
      721 ggctgctttt tctgtcacgt atttcccgtg acggcgttac cggtttccgg gggtaacatg
      781 tgacggagat tatattttta atctccgtgt attcatcata cctgctctta ctgataggat
      841 aactgtgtta tatcgcaggc taccggatgt ggcgctgaat ataacggtca tagataagtc
      901 atacatgccc tgctcagggg attagcgata agacggaccg ggagagtcat ctcccggttt
      961 ttctttatcc tgccgcgaat acagcccggc tgtctgtcag attcaccgga cataaccgta
     1021 ctgttatgca tgccactgtc accttctgct tcttgaaatt catcacattt tttatctctg
     1081 ttggcttacc cgggatgtga aatatgttaa ttctgtgatg tccgtttgaa agacagggca
     1141 actccttctt acaccactat aataataacg atctgccata taagtctgat cccccggcgc
     1201 tccgcaacgg gggttatttt tatcggcact tacaccggca tgcagatgct ctcttcaggg
     1261 caaaatcccg ccatgcttca cagggtgcca gccgggccgc attctgccag gcctcagcgg
     1321 cttccctgaa aaaaccacga atctcctgac ggacggcatg acgccccacg cgctgatagc
     1381 gataaatacc gctgtgctca gataatccac tggatacagc cattttttat cccccctctt
     1441 gacccggctc ccggattatg tcagaaacat tatgaaacat ccctgaacgg ttgggtgttt
     1501 ttctccatca aaactctgga tgtctgtttt cccgattttt tcgctgttgc cctctgtcat
     1561 acagagggcg tttttattat tctgccagga tcgcccgcag ctcatccttg atccggttct
     1621 gtctgcgcac cttttccagg cgcgcctgcg catactgttc ttcttcctgt gtgatatgtt
     1681 ctgtcacata gccttccgta tcatagcggc aggcgccggc tttcattgca cacagataac
     1741 tttccgaacg ggtgatggct ttcagcgccc ggctgagttt tttatgagag agcgggatat
     1801 tccgctgaaa cacctcatcc agtaaaacct cccggatacc acaggcaaga agccggggcg
     1861 tgttaccgtc aaacaatccc ggccaccagg gtttcagagt gttcaccgcc tcatccagcg
     1921 agggcagcgt cagataaatg gacagcgcct gtctggcctg tgctttcttc gccgccagct
     1981 ctgcttcccg ggcagccttc tccgccagtt tctgcttttt caccttccat tttggtggcg
     2041 tggtgacatt gatgatggtt ttccggctcc gggtcggtgt ttccccttcc gttttccgtt
     2101 tcagtgtcag taccggtcgt ttctgctctg tcatgattgc cctgaccttt gtgaaaatat
     2161 gtcccccagg tcgttgaaac tactcactat gatattaagc tgacctcaac gacctctgac
     2221 atattaatac cgacaattaa agatgttttt taaaccattg cgtctgtact gccgccacac
     2281 gttcaaaaaa cgcgccttca taaatatcat aatggcatgc gtcagcttcc tcgtataatt
     2341 ccttagtacc agaagcaaca gcatcatata aggctcttcc ctgttctggt ggattaacac
     2401 tatcctggcc ggcaataaca accaaaactg ggcactgtac tttggctgcg ctctctgccg
     2461 gtttatattg caacgtctcc ataaccgtaa ggaaaggtat ttttatatcc atttcaggat
     2521 actgaccttt taccttctcg aagaatacct ttgattcatt atcactcagt acccgggtaa
     2581 cccccacaaa catctctttc ccggtatttt tcttcttttc tgccatttta ttcagcgtgg
     2641 acagaaaaga agctttctcc agttcattca tttctccggt aaccagaacc tcaccgtcag
     2701 caaaagccag ctggctgaca atacttttca ctcttttatc ctgcgcagct gcattgaata
     2761 catgacaccc acccagcgat gttccccata atcctatacg ctgattatca atacatacct
     2821 gcttttctgc ccaattgata actgaaataa tatcttcggt ctgcattgcc ggaaccagac
     2881 gacctcgttc cccttcactc tcaccgaatc cacgataatc aaaagtaatc gttgcaaacc
     2941 cggcttctgt aaacgcattc gcaaaggaag gcagcagaac attccggata ccgcaaaatc
     3001 catggcataa gagaatcaaa ggatgtttta tatttccttc cgggacacga aatgtcagag
     3061 caataccttc agggagtttg tgatccgtaa ttttcataaa tatcctgtgt tatgttgttt
     3121 attaataagc agaatcagcg ccagcatcat accagtcaca cagccagaac gcccagcaca
     3181 gtaagatgac aggcgtagaa taccgggaaa aaatctccgg gccagaaacg gggtaatgat
     3241 ttccctgtac acgacaccag accaacggtc agcaccgtca ttacaagacc agctacggcc
     3301 gccacatcac tggaggcaag gttaagtgcc ggaataacgg ccagcaggca tgcaagcagc
     3361 gccagacgtt ccgctctgtc ttccgcccgg tacagccggt gactgactgc cagcatcagc
     3421 agcccggcaa tgccgtaact ggtgccggac aaaggccccc acagtgccat caggagtatg
     3481 gccgcggcag tacgccacag gctgcgcgtc tcacaccacg tcagcacctg tgccgccact
     3541 gcaaaggcaa acaggatatt cccctcatac cagggaaagc cggcaacgta atacgcgaac
     3601 tgggcaatca cagcccatcc ccacagccgg ttaatagccg actgccggat attcgcatga
     3661 cgggacagat tcagtcccca gaccagcgca aacagcggaa acgcgccccg cccggccaga
     3721 aacatccacg tctgctccag atgcagaatg cggtttgcat ggtccagcac catcagcacc
     3781 agtgccaccg ttttgatgat atcccgctgt ccgggtgacc agaccagcaa cgactgcagc
     3841 caggtaccgg tccgggcagc cagtgaatcg ttacgtgtcg tgttcgtgtt atccgttgtc
     3901 atcagcgttt gtcttcctgt atcagtctcc gcccagggtt ttctctttct gcaggtcacg
     3961 gaccatatcc cgctccatct gctgaagtcg ctcctgcagc agattttccc gggcaacctc
     4021 ccggacagcg tcctgcaccc gttgagactc acgcagcaca ctttccggca gcgcagtttc
     4081 ccgttctgat gacgaagccc tgtcctgctc ctgcccggca atagccctga cagcctgctc
     4141 tgttttaccg tccggctggt caggtttttc ctctgccatt tttctgacaa tctcatccgc
     4201 acggcgttcc gtttcacgac ggatggcctc ttcagcctgc cgctgtgcca gcacctctgc
     4261 cgtgaccggc tcaccgtttc cggcccccgg ctggacgctg ttgtccggga tatcccccca
     4321 cacgcgacca ccggtgatgg cacctggatt ccacggacga ccttcaccgg cgattctcac
     4381 caccactccg ctgtcggggt tatcacgggc aatccggaca ccatcctgca tattatcagc
     4441 cagcaggctc tcgccgttac ggctgccctg cagggccaca aactgcgcat ccccgctgcc
     4501 tttcacccgt ccttcaccgc tgaatccccg cagcccgttt ccgtcatccg tggtcagtgg
     4561 gttcagccag ataccggcgg acttgccgtt acggtcaaaa gccggcaggg ccacatacgg
     4621 ctgcggatat ttgcgccccg gtgcaataaa ccgtgccgga ctgtcgcccc cggccagtcc
     4681 cgcctggcgg agaacagcac ggccagctgc cacatcccgc agctcccgtg ccgtactgaa
     4741 caaccgctct gcattcatga cctcccggtc agcccccggc tccagcacat cgtgggccgt
     4801 tcctttctgt acggctttgc tgatggcatc cgtccagccc tgacggttat cggtgtacac
     4861 ctgcacatgc tgcttcatac gcgacagggc cacataggct gactcaaagc cggccatctg
     4921 tttccggtta ccttccgtgc cttcaagcgc gatggcaaag gtttcacttg ccccctgtgc
     4981 accgtgagcg gtgatggcat aggcaaggtc aatatgttgc tctgcccgct ccaggccggg
     5041 gcgaatcacc cgggtctgct gtccgtccga cagcgtgaca ctgtcaccgg aaaccgctgt
     5101 caccgtccag acgctgttgg ccacataacc gcgctcccgg tcactcttcg tgaagcgcat
     5161 ccggtcaccg gtccccaccc ggatggtgtc cggggtgtac agtgtgacgc cttcagccac
     5221 cgcctcccgg ggcgaaatca gccgcgtgtt accttccgca tcctccaggg ttatcagccc
     5281 gtcatccttg ctgataccgg caatccggtg atacacacta tccaccaggg caagggcatc
     5341 cgggttattc tcccaggtgg agagacgacg cagttctccg tcacgtatat tcgctgtgtt
     5401 caggacaggc accatgacct gctctttgcc cagctcaccg gccttttccc gtgcatcatg
     5461 aatcatgctg ttcagtacgc gccggtcctc attcaggtgc gtgacaatca gcgtctgctc
     5521 ccgtgcttcc ggtgtcctgc cggtatagtc gcggacaatg gcttcataca gtgtcatggg
     5581 aacatccggg aaagcctcgc ctttcagcat cgccttctgc tgcgcttctg ccagtttcgc
     5641 ttcctggctg tgactgaact ccgtcacgga gtgctccggt gcccatgcac cctcctgacg
     5701 tggcacctga gacggtttca cactctcaag cccggacagt gccctttcca catcccggtt
     5761 aatcaggctg tataccgcct cccgcagttc cggggtctga cgcacaatct ccttcatgat
     5821 ggccacatcg gcagcactgc gcgtctgctg gagacggaaa ggctgaccgg gcgcgatggc
     5881 ctgcagctgg tccgtgtcac cgctggccac ggcacgacca ccaccggccg caatcagggc
     5941 gtatgcccgt gccatgtcgg tattgcccac cattgagctc tcatcgagca ggaacagcgt
     6001 gttgctgaaa tccggcgttt ctccgctgcg ctgctgcagc tgcgtgtcat gcagaaagga
     6061 cgccagtgtc tgtgcatcca cgccggcgct gcgcatctcc ccgaccgcac ggtgcgtggg
     6121 gcccagcccc acgacacggg gacgctcact ctccggcagc atcttcacgg ctgacatcac
     6181 cgcccggaac tgtgtggtct tacccacgcc ggcataaccc tgcaccaccg tgaaacggtc
     6241 ggacgtttcc agtatcatgc gggtggcggc acgctgcccc gacgtcagtg tctccatgag
     6301 ttcgccgggt accctctcca tcagcggcgt gaccgcctcc ttaccttcga gaatatggcg
     6361 aagaatgctc ttttctgcct catacgacgc acgggaaacc agcaggcctg tgccatagcc
     6421 ttttgccaca tccacataca gtaaatcacc gcgttttatc tgcgcattga tttcccctcc
     6481 cagttcagta aaaccggttc cttctgcagc aaacgacttt gcctctgtca gcaggtccac
     6541 catgctgaag gccaggtttt tactttccag cacaggaagg gcaagatgaa tggcctgctg
     6601 tgccggcgtg tgaagtgcgg acttctgatg actgatggca ctttccagca atgtctcacc
     6661 ggcacgcgcc tttatctgct cagaaaccac cgtaaaggag ggatggcggg caagtttttc
     6721 tgcagtacgg gtttcatcca gcgaggaata cagccggaca tcatgaccgc tgcgggccag
     6781 accgttcagg gtggcgttgt ccattgccat ctgtgtgacg gaggcaaaaa ccgtcgcact
     6841 gtcgctgacg gaatgcccgg gcgtttccac ccagccgttc tccagcttca gtgccgtgaa
     6901 cggtgaatca gccacaggca gggaggccgg ttcagcccgc cccggcacaa caaccgtcat
     6961 cgcatcttca ctgacggatg ccacctgcag gcggtcaccg ccggagacgc ggagtccggg
     7021 aattttccct gtcaccctca gacgctcgcc gtctgccacc ggcatttttt ccggccggaa
     7081 cagcgaccag ctgctgtcca gagaagaaat acgcaccacc tgcgtttcgc cctgcgcatc
     7141 ccgcagggtc aggctgtgac tctgcgctgt cacccggtcg ataacatagc ggtcatgact
     7201 gcgtgtctcc gggttccact gctccatcac catccccggg cggtacatat cccgcagata
     7261 acggctccgg ctgtccagcc agaccggtga cagtgccgtc atggtcacct cctgatgtcc
     7321 gagtacgccc tgcgttttca gttcactgcg aatggcctgt gtcagtatgg cctgttcccg
     7381 taccccgctg acctgtgcca cgctctcttc tccggctttc acgctggccg caaaatctcc
     7441 ggccagccgg gcatagcgga cattacggtc cggttcactg atgatggttg ccggtcgctg
     7501 ttctcccccc tgccagcgat atgtgttcac cccggcatcc ttcatggcca tcagtgcact
     7561 gcctgtaccg gttcgctgcc cgctgtcggt tatcaggacc tgtacgttat gacgtgcggc
     7621 accgtccagc agggttaacg tctctttcag ggagagtttt tcgccctggt cgacgataac
     7681 ggtactgccc ggcgtgaagg tcatgccctc cagcagctga cgacgtccgg ttatcagttc
     7741 accggacagc cgttcatcct gcttcaggtt catctgtgag cgacggtcag cagcgataat
     7801 ctgcacctcc cgcccctgct cccgggccat catgaccagt tcagccaccc gctcacgctg
     7861 cccggctgca ccgccctgcc cggacacaat agccagcgac gggcgatcct gtgccagaac
     7921 actgaccgca tcgctgtaac cggcagtccg gggaacactt ttctccggat gtacggtcac
     7981 ccggttctgt ttcatgatgt cacgactgag tgcccggact gacagctcat cgagcacatg
     8041 aatcccggac gtgaacagcc ccttctcacg atcgaggggg acaagctgct cacggctgat
     8101 ggcctcatcg ataccggcgc gcgcccgctc aatcacaccg gcttccggcg gcagtatgcc
     8161 gaccgtcctg gccagcacgt ccgtatacgt gaactgcact tttcgttcac ttaatccggc
     8221 aatggcctgt gtcaccgcct gctgcacatc cggcccgtcc tgtgaagcgg gtccgggtgt
     8281 ctgcgtgcgg gtctccgccc gttggtccgc cgcgtcacga tacgcccgga tgtcgaaccc
     8341 ggtttccttc agcgtctgca tccattcagc cattctgact tcaggatcga cgtgctgttt
     8401 ggattttcgc gtatccagtg ccgccacatc ccgggatttc agcgaggcgt cttccccgac
     8461 ggcctcccgg atagtctgtg agcgtccgga aaaggcctcc accggtacac ccggcatttc
     8521 ccacataccg tgcttaccaa ccacttcggt ttcatagccc agcgcctcaa cctgctcttt
     8581 cagtttttcc cggtagagcc tgccaaaggc aatctgatta gcgtacacat tctcaatgaa
     8641 ccccgttttc cccactttgt cactgctcag tgtcttccat tcgccgttat gctgtgtgac
     8701 attagccacc accgcatgcg tgtgtaactg tggttcctga tcgcgactgg tgtcgtggtt
     8761 aaacagtgcc atcaccagat taccggtcag caccgtttct gactgtccgt ccgtcatcac
     8821 ccgtgtggag gccagtgcct ccacctgacg gacagcaaaa tccacggcct ggttatgtgc
     8881 ttcaataaga cgtttgtccc cccctagcat ggccatcatg gagacacttt tgggggcgga
     8941 gaaggtcagg tcgtagccgg gacgatgcct gttactgcca tcctgcatgc ggcttaaatc
     9001 cgccccgtcc ggcagccttc cctccagaag gcgggtaaag acatccttat cgacactgcc
     9061 ctgcagcccc agttgttcag cccccctgcc ggcccagcgt tctcccatgc tgcccagcac
     9121 atagtaatta tccttgtcgg tataatagtt cccggcactt ccggccgatc tgacctgcgc
     9181 gatactcatc ataaacgtat atccctttga catatcccgg tatcaatccc acaatggata
     9241 tacacaagac atatccacat aaggaggcaa ataatggaaa ccaccgtatt tctcagcaac
     9301 cgcagccagg cggtcaggct gccaaaagcg gttgcattgc cggaaaacgt aaaacgcgtt
     9361 gaagtgattg ctgtcggacg aacccgaatc attacgccag ccggagagtc atgggatgaa
     9421 tggttcgacg gaaacagcgt cagcgacgat tttatggata acagggaaca gcccggtatg
     9481 caggaaaggg agtcattctg atgctgaagt ttatgctcga taccaacatc tgcattttca
     9541 caataaagaa caagcccgcc agtgtcaggg agcgttttaa cctgaaccag gggagaatgt
     9601 gtatcagttc ggtcaccctg atggagctga tatatggtgc agaaaaaagc cagatgcctg
     9661 aacgtaatct cgctgtgatc gagggatttg tttcccgcat tgatgttctg gattacgacg
     9721 ctactgcagc cacacacacc ggccagataa gagcagaact tgcccgtcag ggacgccctg
     9781 tcgagccatt tgatcaaatg atcgcaggtc atgcccgcag tcggggactg attattgtaa
     9841 ctaataacac ccgggaattt gaacgtgtgg acggtctgag aattgaagac tggagctgac
     9901 ctgttcatca gaaatcatct cccggctcaa catcctcccc gcgctcccgg tgcacgttga
     9961 tatttacctc ttcacggcgc tgcatctgct gctggatatc cggattctgc tcccgttgcc
    10021 atgcctcata aacggccata tccaccactt caccggattc actgatcccg ggtggcagtt
    10081 gctgttccat ctcttcttcc ggtttcattt tcagctcctg ctcgatcccc cctgccggaa
    10141 cattcacacc tgcatctgac ttcttatcgt tgatggcagg agacaccggc tgttgcggct
    10201 gttgcggctg ttgcggctgt tgcggctgtt cagcctgagt cacgtcttct ccggaaacaa
    10261 cctccgggac atccggttcg aagaggctgg ccatctgacg accttctgct tcccttgcgg
    10321 caagtacggc actcagacgg ttctccattt caggattgat gtcacgcgga ataaactccg
    10381 gagcgacctt cggacgtgcc tgatatttca gggagagttt tactgccgga tacggtccgg
    10441 gcagggtgac ataacaggtc agatccggca gagactgaat gtcggaataa ctgaccagcg
    10501 tctgacgctc catatcctta ccggtcgata ccccgtcacg taccggatca gcaccgtagg
    10561 aatactgctc gctggctttc aggtgctctt tctcaccaat ttcaccggca gcgaactctg
    10621 caatcttatg gctgggagaa cggaaaaagg cacgggtgtt catgacatca aacagcgtgg
    10681 ccgccgcttt ctccccgtag atatcttcca gctgggcata ggactggata ccgaacacat
    10741 aacagccgcc gaacttacgg gcttccggca ggatctccac caggtccggg agtttatgca
    10801 gcgtgggcaa ctcgtcacag aaaaaccaca cacgacggtt acggttttct cccattgcca
    10861 gcaggccacg aatggcaatg gacagccaca tggagatcac cggcttcagg gaggcatgcg
    10921 tgtcggcatt cgacgagatg aacagccagc cgtttttctg atcttcccga acaccacgca
    10981 tccagtcacg gatggtgaag gactcaccgt tatgctcaat cccctgcagg taacggatag
    11041 ctttcacgta gttggtcagc acggcacgga tggagatggc cgttttttcg attttctctt
    11101 ccaccaggtt ggccgccggt gaattacgca ggaaggtacg cagtttttca attttgatgg
    11161 aaagcagtgt atccaccagt ttgctgtagc tgcggttggg gtcattacgc atcaggtacg
    11221 ccgcttccgc aaaaatggta cgtcctgacc cctgccagaa cgggtcctct tttgtgccca
    11281 tcgggatcag cgtgttcgcg gtattatcaa aatccggctg tgtcaggcac tccttccaca
    11341 aatcccaggc ggcacagcgg gcatccagcg gattcaggat tttatcgatg gagggatcat
    11401 agtaactttt aacaaattcc cctgaacggt cataaatcac caccatatca ccgcgctgac
    11461 gggcgtagtt ggccagacga cggataactt ccgacttacc ggccccgacc gtgccgtgca
    11521 gacagaagtt ctggatttca gaatcccgga taatcggcag atcgccaatc cggatatcgg
    11581 aatccttgcc atcttttttc agcatcctgg ccacctcttt cggattgtca gtcagctgac
    11641 gaccacctgt gatttcattt tcgctctgtt gtttaccctg acgccccaga atccaggaga
    11701 caacaaagaa ggtgatcagg caaataacca gggccacgac agacgccaga acgaatgcag
    11761 accatagctg ctcaccgcac cagatcatgt atttatcatg cagtacctga gccgcgttca
    11821 tccggaaggt tttgccgtaa tactggatct catagaccgg ctgggacctg attaaatccc
    11881 gcatgccttc cagcgtggtg caccaccagt aaatacagcc gttcacaaac gtctgccagc
    11941 ttatttttac ccataaaacc agaccaacga ggatccagaa aaaaataaac aggcagtaaa
    12001 gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat ggacgcaatc tgaccgccct
    12061 gggtcatatc ctttgcgtta aaactcatga tgaaactccg gtaaagtcag gtacagagca
    12121 gaaaagagcg gtgtggaata cattcacaga acaaaccatg tataccctgt ataccggtgg
    12181 tgatagccgc ctgctggtct gccggaagaa actttttcca gcacgatatc agtcaggcgc
    12241 aatatttcag accagtcact ctcgtgtgaa tactggatct gaacaaaaag gcgtacccgc
    12301 tcacgataat ccagagccgt gcccggttca taaaagtggc cactgtcagg agaaacacgg
    12361 gatgttttat tgatgagatc gtatttatcg gcatgagtct gtgacgacag agaccaggta
    12421 atattttctt cctgctcacc atcaatacgg ctactcccgg gcgtccaggc aatttccttc
    12481 gcactggtca cgatgatctg tctggagact tctgccagtt ttttgggggt acagaatacc
    12541 ggggccagca cacacaggga caacaaatta cagcggccct cttccggctt aagcggattg
    12601 ctcccggatt tctcaggaaa agatatt
//
LOCUS       DAZUCC010000147        12502 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZUCC010000147 DAZUCC010000000
VERSION     DAZUCC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.148"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            267..767
                     /gene="tssB"
                     /locus_tag="ACHY09_003540"
     CDS             267..767
                     /gene="tssB"
                     /locus_tag="ACHY09_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFS3886466.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            826..2364
                     /gene="tssC"
                     /locus_tag="ACHY09_003541"
     CDS             826..2364
                     /gene="tssC"
                     /locus_tag="ACHY09_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFS3886467.1"
                     /translation="MSVQQEYATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVKIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDVNLSLVSQMP
                     KAKA"
     gene            2384..3721
                     /gene="tssK"
                     /locus_tag="ACHY09_003542"
     CDS             2384..3721
                     /gene="tssK"
                     /locus_tag="ACHY09_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3886468.1"
                     /translation="MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     SNVLFDRDALTLGKLKTQAVRLRFADGTLIDSDVADILPPACDLRTLEGGSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLTLNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLQEEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRS"
     gene            3718..4383
                     /gene="tssL"
                     /locus_tag="ACHY09_003543"
     CDS             3718..4383
                     /gene="tssL"
                     /locus_tag="ACHY09_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFS3886469.1"
                     /translation="MKKETDINIDVLLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4396..6048
                     /locus_tag="ACHY09_003544"
     CDS             4396..6048
                     /locus_tag="ACHY09_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3886470.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWWVFVSPSGVLRWAGAAVIVLAVA
                     ALLIYRRRQTWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGMYRDTAVLTGQLRRFAHSMAIVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRTIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPLGRAVNRRVEISLVPRSDACQDVK"
     gene            6106..6597
                     /locus_tag="ACHY09_003545"
     CDS             6106..6597
                     /locus_tag="ACHY09_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFS3886471.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVPTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6789..9434
                     /gene="tssH"
                     /locus_tag="ACHY09_003546"
                     /gene_synonym="clpV"
     CDS             6789..9434
                     /gene="tssH"
                     /locus_tag="ACHY09_003546"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFS3886472.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLHPLLDAQSDECPA
                     RQPQVTEPLTSAQPESATTDVPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAAQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     VQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKD
                     EQSDLLSMEQSLENRVVGQRPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            9446..11935
                     /locus_tag="ACHY09_003547"
     CDS             9446..11935
                     /locus_tag="ACHY09_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFS3886473.1"
                     /translation="MGIQNFDHSHHKLKIRGLKSPVDVLTFTGREQLSAPFRYDIEFT
                     STDKTIEPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGVVRAKKGIFISADAQDNAQGQVREMAP
                     AMAILNGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLNQAAILLSAPKGVAI
                     ASGEHLQLAAAKNVIANAGNNADVGAVKNMFIGVGQALSVFVRKAGIKLFANKGAISV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTQGEYNV
                     KAGYYGRKPKAKLTPELMAFPVISAKFDEQVRVIDDDGKFIANVPYYIEDSIGNIYKG
                     FTDSAGCCERIFTEEADNLNVLLGIKALEKW"
     gene            11929..>12502
                     /locus_tag="ACHY09_003548"
     CDS             11929..>12502
                     /locus_tag="ACHY09_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HFS3886474.1"
                     /translation="MVISENTVGTNDIPDSNHDILGPLMPARGFFFIVIDEPGKDGFL
                     VRKIYASPNSPYLTKMNLDPLRLLSETEPERYGLMPKDYSAPTTVAEHVMGNNKSSLI
                     STSSIFPDGSPRFEGKTIFIDVHKAKNAGLKLISTAEIITALEEYKKQYPHLAKRVEK
                     ISGYVDDIDKEVLIEGRKVPAQTIFTPESLK"
BASE COUNT         2973 a         3384 c         3551 g         2594 t
ORIGIN      
        1 taaaagtgca aatttgtgaa gcttaccctg ttattatttt aagtttcatg ccaaattctc
       61 tcaccagata atgccaccct ctcccgaaaa ataatcaaga ggccaaacaa tatctaaaat
      121 gatacaactg tatctattcc cctgaaaaat acattattca tttgtatatt ttcctcatca
      181 ttgcttttta tttaaatcat ccgataatcc cctgaatata attatgtcaa taaccatcag
      241 aaaaagtgga tgatgaggaa aaggatatgg ccgacagttt ccagaatgaa gttcccgccg
      301 ctcgtgtaaa tatcaagctt gatctgcata caggcaatgc taaaaagaaa gttgaactcc
      361 ccctcaagct tcttgccgta ggcgattaca gtaacggaaa agagcaacgt ccgctgtccg
      421 aacgggacaa ggttgatatc aataaaaaca acttcaacag cgtcatggct gagttttcgc
      481 ctgcggttaa tttaacagta gaagatacgc taaacggaaa cggtaatgaa caaaatatcg
      541 cgcttgaatt taaaagcctg aaggattttg aacccgaaca ggtagcaaaa aatattccgc
      601 aacttcgtgt attactcgct atgcgcaatt tactacgtga cctgaaatcc aatctgctgg
      661 ataacgcaac gtttcgccgt gaactggaaa atatcctgaa agacccgacg ctcagttcag
      721 aactgcgtga tgaactggcg aaaattgcgc cgcaggagaa cgtttaaccc gaacgcgtat
      781 tcaggttaaa ccggagaaga aaggatccct aaaaaggaaa tgctgatgtc tgtacaacaa
      841 gaatatgcca cctctgaaac tgcaacactc accaccactg agtccggcgg cgtttatcag
      901 tccctgtttg ataaaattaa tttaaccccg gtctcttcca ttcaggaaat cgatttatgg
      961 caaaacagcg aaacgctggc cgatgcttca cccgatgagc gcgtgacggc ggcgatccac
     1021 gttctgcttt cctgtctggc gaaatcaggc gaggacgtgg ttaaactcga caagagcctg
     1081 ctggattttc atatcgacga tttggatcag aaaatcagta aacagcttga tgcagtcatg
     1141 caccatccgg aattccagaa agtcgagtcg ctctggcgcg gcacatggtt cgtcgtacag
     1201 cgcaccgatt ttcgcaaaaa tgtcaaaatt gaactgctgg atatcagtaa agaacatctg
     1261 cggcaggact ttgatgattc tccggaaatc attcaaagtg gtttatatcg ccatacatac
     1321 attcaggaat atgatacgcc gggtggcgaa cctgtagcct cattaatatc cagctatgaa
     1381 ttcgataaca gcccgcagga tattgccctg ctgcgtaata tttccagagt ctctgctgca
     1441 tcacatatgc cctttatcgg ttccgtcgga ccgaagttct tccttaaaaa ctcgatggaa
     1501 gaagtcgccg caattaaaga tatcggcaac tattttgacc gcgcggaata tatcaaatgg
     1561 aagtcgttcc gcgataccga cgacagccgc tacgtgggat tagtgatgcc gcgcgtgctg
     1621 ggccgtctgc cctatggccc ggacacggtg ccggtacgca gctttaacta tgtggaagag
     1681 gtcaaaggcc cggatcacga aaagtacctc tggacaaacg cctcgtttgc ctttgccgcc
     1741 aatatggtga agagttttgt gaataatggc tggtgtgtgc agatccgtgg tccgcaggcg
     1801 ggtggcgcag tagccgatct gccgatccat ctttacgatc tcggcaccgg caatcaggtc
     1861 aaaattccgt ccgaagtgat gatcccggaa acccgcgagt ttgagttcgc caaccttggc
     1921 tttatcccgc tctcttatta taagaatcgc gattacgcct gcttcttctc agcgaactct
     1981 gcccagaaac cggcgctgta tgataccgct gacgccaccg ccaacagccg tatcaacgcc
     2041 cgtttgccct atatcttcct gctgtcccgc attgctcact acctgaaaat cattcagcgc
     2101 gagaatatcg gcaccaccaa agacagacgc gtgctggaac tggagctgaa cacctggatc
     2161 cgcacgctgg tgacggaaat gaccgatccg ggcgatgaac ttcaggcttc gcatccactg
     2221 cgcgacggga aagttatcgt cgaggacata gaggacaatc cgggcttttt ccgcgtcaga
     2281 ctctttgccg tgccgcattt ccagatcgaa gggatggacg tcaacctttc tctggtttcc
     2341 cagatgccaa aagcaaaagc ctgatcgggc ggtggtgtga tgtatgaaaa tttgtcgacc
     2401 actgtgggct gaggggacat tcctgtcttc tcagcagttt cagcagcagg cccgctggga
     2461 agcgttcagc aatgacagca ttgcgcagct ttgtattcgc catccgtggg ggatctcaaa
     2521 cgtgctcttt gaccgggatg ccctgacgct cggaaagctg aaaacgcagg cagtacgcct
     2581 gcgttttgct gatggtacgc tgatcgattc agacgtggcg gatattctgc cgcctgcctg
     2641 tgacctgcgc acgctggagg gcggcagcgc gattgttttg ctggcgctgc cgcttgcgca
     2701 tggtaacggc ggtaatctcg gtcagggcga gcaggctgaa aggccgctgc gctatcgtca
     2761 ggaatggcaa aaggtgcagg atatctacgg cagtgacagt gaagatatgg ccgttgagcg
     2821 ccatgcactg tccctgcgct ttgcccatga taacaatcag gattacatca cctgtccgct
     2881 ggcgcgtctg gtgcgggatg ttcagggcaa ctggacgcag gatgagagct atattccgcc
     2941 gttactgacg ttaaacgccc atgacgggct ggtacagcgc ctggacacgc tgctgttgca
     3001 gctacgggcg aagtgccagc gcctgatggc gatgcgccgg gaaagcaacc agcgcatggc
     3061 ggacttcgcc gtcgccgatg tctctctgtt ctggctgctt aacgccctga attccgccga
     3121 gccggtgctg agcgatttcc tgcgctaccc ggctgtccac cccgaactgg tctggcggga
     3181 gctggcgagg cttgccggtg cgctgctgac gttttccctt gagcataacg tcagcgccgt
     3241 tccgccctat gtccacgaat cgccgtccac cgtctttccg ccactgttct ccctgctcag
     3301 cgagctgctg gaagccagcc tgccgtcgcg ggtgatcgcg ctggatctgg cgtccctgcc
     3361 gggcaaccgc tggaaagccg acctgcacga tccgcgcctg caggaagaag ccgattttta
     3421 cctgtccgtt cgttcctcac tgcctgcgca tcaggtactg caccagctac cgctggtctg
     3481 caaaatcggt gcgcctgatg acgtcacgct gcttatcaac gtggcgctga acggtgttcc
     3541 actggtgcca ctcacctcgg ttccggcggc gctgccgctg cggctggaga accagtattt
     3601 tgcgctggat atgcacagtg acgcggctaa atcgatgctg gagtccgggt gctgcatgat
     3661 ttatgcgccg ggaacaatgg gcgacctgaa acctgaactg tttgcggtgc tgcgctcatg
     3721 aaaaaagaga cggatatcaa tatcgacgtg ctgctgcgcg acacgtttct gacggtggtg
     3781 gaactgcgtc agggaaccac cgtgcgtcac ggtatcgagc tgtatcgcca ctgtcagcgg
     3841 caggtggaac tggtccgcga acgtctgaaa gatgccggat tcagtcggga gaacgtggag
     3901 cacataactt acgcgcagtg cgccctgctg gacgaaacgg tgctcagccg tagtggcatg
     3961 gacgacggtc aggccatctg gatgaaaaac ccactccagt cgcatttctt caacactctg
     4021 caggcaggcg agctgctgta cgaacggatg aagcaggtgt tgcaggagcc cgcccccgcg
     4081 caggcagtgc tgacctgctt tcaccgtgtc ctgctgctgg gatttcgcgg gcgttatcag
     4141 gatccggtgg ccccggagcg tgaccagctt atttcgacgc tcaacgggct ggtcgcgcca
     4201 tttggcgtgc tgccggaaac ggcggtactg aacgtgccgt tatccccccg tcagcatcct
     4261 ttgctgcatt cgccgttttt ctggctggta acgctgacgc tgctactggc cggtgtctgg
     4321 tggggactgc accactggct gaacgtgctg gtggatgaat tgctgccgca aagcctgcgg
     4381 taaagggagc cgtgagtgag gaacacgctg aaacaggcca tcgtgctgtg gggaatggtg
     4441 ttactgctgg tgctgtggtg ggtgtttgtc agtccgtctg gcgtactgag atgggccggt
     4501 gcagcagtta tcgttctggc ggttgccgca ttgttgattt accggcgcag gcagacgtgg
     4561 acggagatga ccggcgatgc cgggttgtta tcgctgccgc cggaaacgta ccgacaaccg
     4621 gtagtgctgg tctgtggcga tatgtcagcg catctgttca ctgacagccc ggtccggcag
     4681 gtttcagaag ggctgtatct gcatgttccc gatgaagaac agcttgtggc gcaggtggag
     4741 cgattactga cccttcgccc ggcgtgggca tcgcagcttg ccgtggcgta taccgtcatg
     4801 cccggcatgt accgggatac ggcggttctg accggacaac tgcgacggtt cgctcacagt
     4861 atggcgatag tgcgtcgccg ggcgggcgta aacgtaccct ggcttctctg gagcgggctg
     4921 tccggctcgc cgttgccgga gagagcgaat tcaccgtggt ttatctgtac cggcggcgaa
     4981 gttcaggtag caacatccgc agagaccacc atgcccgcgc agtggattgc acaatccggc
     5041 gcacaggaac gcagtcagcg actctcttat ctgctgaaag ccgaaagcct gatgcaatgg
     5101 ctggatctga atgtgctggc ggaactgaac ggcccggagg cgaaatgtcc accactggcg
     5161 atggccgtgg ggctggtccc ctcgttgcct gcggtggata acaacctatg gcagttgtgg
     5221 atcaccgcca gaaccggcct gacgccggat atcgcggaca ccggcacaga cgatgcgctg
     5281 ccattcccgg atgccctgtt acggcggttg ccgcgtcagt cgggctttac cccgctgcga
     5341 cgggcctgcg tgaccatgct gggcgtcacc accgtggcgg gtatcgccgc gctgtgcctg
     5401 tcagccacgg caaatcgcca gttattacgg caggtcggtg acgatctgca ccggttttat
     5461 gccgtcccgg tggaggaatt tatcaccaaa gcccgtcacc tgtcggtgct gaaagacgat
     5521 gcgaccatgc tcgatgggta ttaccgggaa ggagaaccac tgcgcctcgg tctggggtta
     5581 taccccggcg aacgcatccg ccagccggta ttacgcacca ttcgcgactg gcgtccgcct
     5641 gaacaaaaaa tggaggtgac ggcttcgctt caggttcaga ccgtgcgtct tgacagtatg
     5701 tcgctgtttg acgtcggaca ggcccgcctg aaagacggct cgacaaaagt gctggtggac
     5761 gcactggtga acatccgggc aaaaccgggc tggctgatcc tcgtggccgg atataccgac
     5821 gccaccggcg acgaaaaaag caatcagcag ttatcgctgc gacgtgccga agcggtgcgt
     5881 aactggatgc tgcagaccag cgacatcccg gccacctgtt ttgccgtaca gggactgggt
     5941 gagagccagc ctgcggcgac caacgatacg ccactgggca gggcagtcaa ccggcgcgtc
     6001 gaaatcagtc ttgttccgcg ttctgacgcc tgtcaggacg tgaaataaaa cataccgccg
     6061 gaagaaggcg gtgcttcaat cacactaaca aggagagtaa ttctcatggc tattcctgct
     6121 tatctctggc tgaaagatga cggcggcgca gatatcaaag gttccgtgga cgttcagggg
     6181 cgcgaaggca gcatcgaagt ggtggcgttg gatcacgatg tgtacatccc gaccgacaac
     6241 aacaccggca aactgaccgg tacccgtact cacaagccct ttacgtttac caaagaaatc
     6301 gatgcgtcca gtccgtatct ctacaaagct gtgcccaccg gtcagaccct gaaaacggca
     6361 gaatttaagt tttaccgcat caacgatgcc ggtcaggaag tggagtactt caacatcacg
     6421 cttgataacg tcaagctggt cagagtcgct ccgctgatgc acgacatcaa ggatccttcc
     6481 agagagaagc ataaccacct ggaacgtatt gagttccgct acgagaaaat cacctggact
     6541 tacaaagacg gcaacatcat tcattccgac tcgtggaatg agcgtccttc cgcctgataa
     6601 ctgcggcaga caggaaactg tctgcctttt tgttgtgtgc tttgtccagt gccgcaggca
     6661 ccggtactga gcaaagcata cccacaatct ggtgaaacct gatgggcctg tgtgaggtaa
     6721 cggctatggg cggtagcgtg cctgaacgcg gatcgcagga atacagaaca cgataaggaa
     6781 acccttctat gacaggaaat caccccgccg cgctgctgcg tcgccttaac ccatactgtg
     6841 cacgggcgtt ggacgctgcc gcctcactgt gtcagacccg cgcccatgcg gagataacca
     6901 ttgaacactg gctgctgaaa ctgctggagc agggagaagg cgatatcacg gtgattgccc
     6961 gtcgctatga atgggatatc gacacgctct ggcagtctct gctggcgcat ctggacacct
     7021 taccccgctc ggtccgcgaa cgtcctcaac tttctgaacc gctggcagcg cttatcagac
     7081 aggcgtggct gatagcgtca ctggaaggtg acgatccaca aatccgcagc cagcacctgc
     7141 tgatggcgct gacagaaaaa ccgatgctgc ccgcctgtaa tgacctgtgg gtattgctga
     7201 gtctgagccg cgtgcagctt gagcggctgc atcccctgct ggatgcgcag tcggatgaat
     7261 gtccggcacg tcagccacag gtcaccgaac cgctgacatc cgcgcaacca gagtcggcaa
     7321 cgacggacgt accggcaaaa acgctgacgg ggaaacagga tgacgccctg ctggcggtgc
     7381 ttaaccgctt taccgaagac gtgacggaaa aagcccgtag cgggcgaatc gacccagtat
     7441 tcgggcgcga caccgaaatt cgccagatgg tcgatatcct ctcccgtcgc cgcaaaaaca
     7501 acccgattct ggtgggagaa ccgggggtgg gcaaaaccgc actggtggaa gggctggcgc
     7561 tgcgtatcgc cgaaggcaac gtgcccgaca gcctgaaaac ggtgcatatc cgcacgctgg
     7621 acctcggtct gttacaggct ggcgcgggtg ttaaaggtga atttgagcag cgactgaaaa
     7681 acgtgattga tgcagcacag aaatcgccgg agccggtact gctgtttatt gatgaagccc
     7741 ataccattat cggcgcgggt aaccaggctg gcggcgcgga tgcggcgaac ctgctgaaac
     7801 cggcactggc aaggggcgaa ctgcgcacca tcgcggcgac cacgtggagc gaatacaaac
     7861 agtattttga gcgcgacgcc gcgctggagc gccgcttcca gatggttaag gttgacgagc
     7921 cggatgatga caccgcctgt ctgatgctgc ggggactgaa ggcccgttat gcacagcacc
     7981 acggcgtgca tatgctggac agcgccattc agaccgccgt gcgcctgtcg cgccgctatc
     8041 tgaccggacg ccagctgccg gacaaggcgg ttgatttgct ggataccgcc ggagcaagag
     8101 tccgcatgag cctcgacacc ctgccggagc cgctgacgca gcttcatgcg cgactggcgg
     8161 cgctggatat cgagcgggag gcgattgagc aggacagcgt gttttatccc gaaaccagcc
     8221 cggagcggct ggcagaactg accgatttgc gtgatgagct gcaggcagaa gccgggcatc
     8281 tggaagcgca gtatcagcag gaaaaggcac tggtgcagca gattatgacg ttgcgtcagg
     8341 aaggaacaga cagctctgaa ctgcaacagc aactgcggac gcatcagggc tttgcaccgc
     8401 tgctggcgct ggatgtggac gcccgcgccg tcgccacggt ggtggcggac tggactggca
     8461 tcccgctctc ttccctgctc aaggacgagc agagcgacct gctcagtatg gaacagagcc
     8521 ttgaaaaccg cgttgtcggg caacgcccgg cgctctgcgc catcgcacag cggttgcggg
     8581 cggctaaaac cggcctcacg ccggagaacg gcccacaggg ggtattcctg ctgaccggcc
     8641 ccagcggcac cggtaaaacc gaaactgcgc tcacactggc cgacaccctg tttggcggtg
     8701 aaaaatctct tatcaccatc aacctctccg aatatcagga gccgcatacc gtctcccagc
     8761 ttaaggggtc tcctccgggc tacgttggct acggtcaggg cggcgtactt accgaagccg
     8821 ttcgcaaacg cccgtacagc gtggtgttgc tcgacgaagt ggaaaaggcg caccgcgacg
     8881 taatgaacct gttctatcag gtatttgacc ggggttttat gcgcgacggc gaaggccggg
     8941 aaattgactt ccgcaacacc gtgattctga tgacggctaa tctgggcagc gaccacatca
     9001 tgcaactgct ggaggaaaaa ccggacgcca cggacgcaga cctgcatgaa ctgctgtatc
     9061 ccctgctgcg cgagcacttc cagcctgcac tgatggcgcg ttttcagacg gtgatttacc
     9121 gcccgctggg acaggaggcg atgcgcacca ttgtggaaat gaaactggcg caggtggttc
     9181 gtcgtcttca tcagcactat gggctggaaa cggaaatcaa cgacagcctg tatgacgcgc
     9241 tgaccgccgc ctgcctgctg ccggacaccg gtgcacgcaa tatcgacagc ctgttgaacc
     9301 agcaaatcct gccggtacta agtcagcagt tgctggcaca gcaggccgcg caccgtaaac
     9361 cagcccaact acggcttggc tgggatgagg aagacgggat tgtactggag ttcgctacgg
     9421 aagagatgca ataacggagg cttttatggg tattcagaat ttcgaccact cacaccacaa
     9481 gctcaaaatt cgcggcctga aaagcccggt ggacgtgctg acgtttacgg gccgggaaca
     9541 actcagcgca cccttccgct acgacattga atttaccagt accgataaaa ccatcgagcc
     9601 ggagtcggtg ctgatgcagg atggcgcatt cagcctgacg gcaccgccgg tacagggaat
     9661 gccggtacag actgccctgc gcaccctgca cggcgtcatt accggcttta aacatctctc
     9721 atcgtcacag gacgaagccc gttacgaagt gcgccttgaa ccccgtatgg cgctgcttac
     9781 ccgcagccgc cagaacgcca tctaccagaa ccagacggtg ccgcaaatcg tcgaaaaaat
     9841 cctgcgcgag cggcaccaga tgcgcggtca ggacttcgtg tttaacctca aaagcgaata
     9901 cccgtcccgt gaacaggtga tgcagtacgg tgaggacgac ctgacgttta tttcacgcct
     9961 gctgtcagaa gtgggtatct ggttccgctt tgccaccgac gcccggttga aaatcgaagt
    10021 tatcgaattt tacgacgacc agagcggcta cgagcgcggg ctgacgctgc cgcttcgcca
    10081 tccttctggg ctgtttgatg gtgaaacgga agccgtatgg gggctgaaca ccgcgtacag
    10141 cgtggtggaa aaaagtgtct ccacgcggga ctacaactac cgtgaagcca cggcggaaat
    10201 gaccacaggc cagcatgacg ccaccggggg cgataacacc acttacggcg aagcctacca
    10261 ctatgccgac aacttcctgc aacaaggcga taaagaggca gcggaaagcg gcgcgtttta
    10321 tgcccgtatc cgtcacgaac gctacctgaa tgaacaggcc attctcaaag gccagagcac
    10381 cagttcactg ctgatgccgg ggctggagat aaaagttcag ggcgacgacg cgcctgcggt
    10441 gttccgcaag ggcgtgctga ttaccggcgt gacgaccagc gcggcgcgtg accgcagcta
    10501 tgagctgacg ttcacggcga taccgtactc agagcgctac ggctaccgtc cggcgcttat
    10561 cccccgcccc gtaatggccg gaacgctccc ggcgcgggtc accagtacgg tgaagaacga
    10621 catctacgcc catatcgaca aggacgggcg ctaccgggtc aacctcgact ttgaccgcga
    10681 tacgtggaaa ccgggctatg agagcctgtg ggtacgccag tcgcgcccgt atgcgggaga
    10741 cacctacggc ctgcacctgc cgctgctggc gggaacggaa gtcagtatcg cctttgagga
    10801 aggcaacccg gaccgacctt atattgcagg cgtaaaacac gactcggcac acactgacca
    10861 tgtgaccatt cagaactata agcgtaacgt cctcagaacc ccggcgaaca acaaaatccg
    10921 cctcgacgat gagcgcggca aagaacatat caaggtcagt accgagtacg gcggcaagag
    10981 ccagctaaac ctcggtcatc tggtggatgc cgggaaacag caacgcggcg aaggctttga
    11041 actgcgtacc gatatgtggg gcgtggtgcg ggcaaagaaa ggcatcttta tcagcgccga
    11101 tgcgcaggat aatgcgcagg ggcaggtgcg cgaaatggct cctgcaatgg cgatactgaa
    11161 tggcgcacag tcacagatga agtctctgtc cactgatgcg cagacggcga atgccgatcc
    11221 ggcagatctg tcatcgcaga ttgcgttgtt gcaacagagc gtaaaagatc tgaaccaggc
    11281 tgcgatcctg ctaagcgcac ccaagggcgt ggcgatcgcc agcggcgagc acctgcaact
    11341 ggcggcggca aagaatgtga tagccaacgc aggcaacaac gccgatgtcg gcgcggtgaa
    11401 aaatatgttt atcggcgtgg gacaggcgct aagcgtgttt gtgcgtaaag cgggcatcaa
    11461 gctgtttgcc aacaaggggg cgatctcggt acaggcacag aacgatctga tggaactgtt
    11521 ggcacagaaa tcgatagaga tcaccagtac ggaagatgag atcaagatca ccgcgaagaa
    11581 gaaaatcacc cttaacggcg gtggcagcta catccggctg gatgcctgtg ggatagaagc
    11641 gggaacgcag ggagaatata acgttaaggc cggatattac gggcgtaaac caaaggcgaa
    11701 gctgacgccg gagctgatgg cgtttcctgt aatatctgcg aaatttgatg agcaagttag
    11761 agtaattgat gatgacggaa aattcattgc taatgtacct tactatatag aagacagcat
    11821 agggaatatc tataaggggt ttactgattc tgccgggtgc tgtgaaagaa ttttcacgga
    11881 agaagctgat aacctaaacg tacttcttgg tattaaggcg ctggaaaaat ggtgatatct
    11941 gagaatacag taggaacaaa tgatatacct gattcaaatc acgatatatt aggaccctta
    12001 atgcctgcaa gaggtttctt ttttatagtt atcgatgagc cgggaaaaga tggctttctt
    12061 gtcagaaaaa tatatgcttc accaaatagc ccctacctga caaagatgaa tctggatcct
    12121 cttagattat tatcagaaac agagcctgaa cgctatgggt tgatgccaaa agattattca
    12181 gcacccacta ctgtagcaga acatgttatg gggaacaaca aatcctcgtt gatttctaca
    12241 agctctatat ttcctgatgg ctctccgaga tttgagggga aaaccatatt tattgatgtt
    12301 cacaaagcaa aaaatgccgg gttgaagtta atctcgaccg cagagattat tactgctctt
    12361 gaggagtata agaaacaata ccctcatttg gctaagcgtg ttgaaaaaat ttcaggctat
    12421 gttgatgata ttgataaaga ggtcttgatc gaaggtagaa aagtaccagc acaaacaatt
    12481 tttacaccag agtctctaaa aa
//
LOCUS       DAZUCC010000148        12495 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZUCC010000148 DAZUCC010000000
VERSION     DAZUCC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.149"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(146..598)
                     /gene="yafP"
                     /locus_tag="ACHY09_003549"
     CDS             complement(146..598)
                     /gene="yafP"
                     /locus_tag="ACHY09_003549"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3886475.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFSMRYKPQH"
     gene            complement(595..1650)
                     /gene="dinB"
                     /locus_tag="ACHY09_003550"
     CDS             complement(595..1650)
                     /gene="dinB"
                     /locus_tag="ACHY09_003550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS3886476.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1721..2506)
                     /gene="lafU"
                     /locus_tag="ACHY09_003551"
     CDS             complement(1721..2506)
                     /gene="lafU"
                     /locus_tag="ACHY09_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFS3886477.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            2478..4190
                     /locus_tag="ACHY09_003552"
                     /pseudo
     CDS             2478..4190
                     /locus_tag="ACHY09_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4408..4905)
                     /gene="rayT"
                     /locus_tag="ACHY09_003553"
     CDS             complement(4408..4905)
                     /gene="rayT"
                     /locus_tag="ACHY09_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFS3886478.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKQ
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLQNMWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
     gene            complement(5082..5831)
                     /gene="yafL"
                     /locus_tag="ACHY09_003554"
     CDS             complement(5082..5831)
                     /gene="yafL"
                     /locus_tag="ACHY09_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS3886479.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            6143..6883
                     /gene="dpaA"
                     /locus_tag="ACHY09_003555"
     CDS             6143..6883
                     /gene="dpaA"
                     /locus_tag="ACHY09_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFS3886480.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6854..7621)
                     /gene="yafJ"
                     /locus_tag="ACHY09_003556"
     CDS             complement(6854..7621)
                     /gene="yafJ"
                     /locus_tag="ACHY09_003556"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFS3886481.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7737..8315)
                     /gene="lpcA"
                     /locus_tag="ACHY09_003557"
     CDS             complement(7737..8315)
                     /gene="lpcA"
                     /locus_tag="ACHY09_003557"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFS3886482.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8555..10999
                     /gene="fadE"
                     /locus_tag="ACHY09_003558"
     CDS             8555..10999
                     /gene="fadE"
                     /locus_tag="ACHY09_003558"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFS3886483.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11042..11488)
                     /gene="ivy"
                     /locus_tag="ACHY09_003559"
     CDS             complement(11042..11488)
                     /gene="ivy"
                     /locus_tag="ACHY09_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFS3886484.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            11669..12439
                     /gene="yafV"
                     /locus_tag="ACHY09_003560"
     CDS             11669..12439
                     /gene="yafV"
                     /locus_tag="ACHY09_003560"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFS3886485.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFRLW"
BASE COUNT         2927 a         3234 c         3346 g         2988 t
ORIGIN      
        1 ccagggcaag aaaaaaagaa ttaatcctgg aaaggtctgc aaagagtcat tgataaagga
       61 tttgtcatga tttacctcct tgcagtttgt ggaataagaa aacgcgcaca taccaggcgg
      121 gtgttatttt cattgcaagc tggatttaat gttgcggttt atatcgcata gaaaaattag
      181 taaaccacgc tcccctgcat tcaacgtgct gctgcttaac tgtctgaaaa ccataacgtt
      241 caaaaaaggg ttttgcggtt atgctggcgt ccaccgtaag ttcggattca gacttaatca
      301 aaggttttaa caaggcgctg gcaaccccgc ggcgggtgta ttcagggtca acaaataaca
      361 tatcgatata gtgttcaacg caggtaataa agccgaccag ttttgcatta atgactgcaa
      421 cccgcacttg tgatttcgcg agtttctcct tccagcgaga ttcgtcaatc tgcgcccagg
      481 cggcaatttg ttgtggtgaa taatgctgac tggcggtcat cgtaaccgct ctaaggaaaa
      541 tagcgcatag ttgctgaaaa tcgccaggct gatagtctct tatttgtatg ttattcataa
      601 tcccaacacc agttgtcttt ccatttgcgg gtcaagtaac gtcacatgca gccccaccag
      661 tcgcacaccg cgcccgccgc ggcgttcatc ccaggtttta cgcgcagtgg cgattagatc
      721 agctttattc agccgtggcc agacgtgctc ctgagtagtt tgctgaaaat cgtcgaactt
      781 taatttcact ccctgacggg cgatcagtaa atcaggtttc acctttgcca gacggcgttc
      841 aagttcagga tacagccgct cgataatcgc ttcacattca gaccagtgat gaatatcttc
      901 cgccatcgtg cgttccacgc cgacggattt tcgcaaccgt tcactgttaa cgtcgcgctc
      961 gtcaatcccc tgactacgct cccacaaaat gcggccaaat ttgccaaagc gtttaagcag
     1021 aatcaccaga tcacactttt gcacatcacc gcaggtccgc agccccatcg cttccagttt
     1081 tgccgccgag actttgccga cgccgggaat ttttgccagc ggtaaggttt gcagaaatgc
     1141 cggaacttct gccggcgtaa tcacaaactg accgttgggt ttattcatgt cggaggcgat
     1201 tttggcgaga aactttactg gtgccacgcc cgcagatgcc gtcagttgca gctcgttgaa
     1261 gatcgtctgg cggatttcct gggcgatgag ggtcgcagaa ccgtggcaat ggacgctatc
     1321 agtgacatcg agataggctt catccagtga caacggttca atacgcgagg tgtagcgcga
     1381 gaagatttca cggatatgat ttgaggcttc tttgtaggcg tcaaagcgcc ccggaagcaa
     1441 ggtgagatgc gggcataatt tgagcgccat ccctgtcggc atagcgctac gtacgccaaa
     1501 tttacgcgcg ggataattgg cggtgctgat taccccccgt cgttcgcggc tgccgccaat
     1561 agcaataggg atatcgcgca gggcgggatt gtcgcgcatc tccaccgctg cgaaaaagca
     1621 gtccatatcc acatgaatga ttttacgcat tgctcacctc tcaacactgg taaagtatac
     1681 agtgatttca gggtttgaga aatgcgtaaa gattcagcat ttaacgcgct cgctgcgaaa
     1741 gcacctgctg cttatccagc ttttgcacca gcggctgcac cactttatcc ccatgctgac
     1801 caaagtattg atacagcgta tctgacgcac ttttggtcag caccataatc tcaatgcgcc
     1861 ggttgcccgc gctttgcgga tttttggcat ccagcagcat ctggtccgcc attgcgctta
     1921 cctgcatcac tttattttcc ggcattccgg cctcttccag cacccgacga gccgaaagcg
     1981 cgcgatcgcc cgaaaggttc cagttgttgt agatattgtt tttgtaggcc atcgcatcgg
     2041 tatgcccggt aataataatt ttgttatcga gcgagtcgaa cactggcgcg agctccacca
     2101 gcagcgtttt aaagaacggc ataatcttcg cgctgccgcg ttcaaacata ttgcggttct
     2161 ggtcatcttt aatcagcacg cgcagtccct ggggaacaat ctccatctcc agattcgctt
     2221 ccatatgtgc atcacgggca atggtgttga tactggtcgc cagctcgcca agctcggtgg
     2281 cggatttctt cttcagcagc gccgttttct cgttgacgtc gcgcgctttt ttctccgtct
     2341 cttccggcac cgccacggtg gcaggttttg gcggatgaga cgggctgatt ttatttaacg
     2401 gcgacaaccc accaccgtta aaaatcgact gcccgtgcag cgcggcgata atgctttcac
     2461 gctcggattt actgacgcta ttgacaatca actttatcgt cgtcaccaaa ggggccgagc
     2521 gtatttccga ggtttccgcc cgctttacct tagacgcgat gcccggtaag cagatggcga
     2581 ttgacgccga tcttaacgcc gggttgatca accaggcgca ggcgcaaacc cggcgtaaag
     2641 atgttgccag cgaggccgat ttctacggcg caatggacgg ggcatcgaag tttgtgcgcg
     2701 gggacgccat cgccgggatg atgattctgg cgatcaacct gatcggcggc gtctgtatcg
     2761 ggatcttcaa atacaacctg agcgccgacg ccgccttcca gcaatatgtg ctgatgacca
     2821 tcggcgacgg gctggtggcg cagatccctt ccctgctgct ctccaccgcg gcggcgatta
     2881 tcgtcacccg cgtcagcgac aacggcgata tcgcccatga cgtgcgccac cagctgctgg
     2941 caagcccgtc ggtgctctac accgccaccg gaattatgtt tgtactggcg gtggtgccgg
     3001 gaatgccgca cctgccgttt ttgctgttca gcgccctgct tggctttacc ggctggcgga
     3061 tgagcaaacg cccgcaggcg gcggaggcgg aggagaaaag tcttgaaacg ctaacccgca
     3121 ccatcactga aaccagcgag caacaggtca gttgggaaac cattccgctg atcgaaccta
     3181 tcagcttaag cctcggctac aagctggtgg cgctggtcga taaagcgcag gggaacccac
     3241 tcacccagcg gattcgcggc gtgcggcagg tgatatccga cggcaacggc gtgctgctgc
     3301 cggagatccg tattcgggaa aacttccgcc tcaagcccag ccagtacgcc atattcatca
     3361 acggcattaa ggccgatgaa gcggatattc cggcagataa actgatggcc ctgccctcca
     3421 gcgaaaccta cggcgagatt gacggcgtac tggggaatga cccggcatat gggatgccgg
     3481 tcacctggat tcagcctgcg cagaaagcga aggcgctgaa tatggggtat caggtgatcg
     3541 acagcgccag cgtgattgcc acccatgtga ataagattgt gcgcagctat attcctgatt
     3601 tatttaacta tgacgatatt acgcagttgc ataaccgcct gtcgtcgatg gcaccgcgac
     3661 tggcggaaga cttaagcgca gcgctcaatt acagccagtt gctgaaagtg tatcgcgcgc
     3721 tgctgaccga aggcgtttcc ctgcgcgata ttgtcaccat cgccaccgtg ctggtcgcca
     3781 gtagtgcggt gactaaagat catatcctgc tggcggcaga tgtgcgcctg gcgctgcggc
     3841 gcagtatcac ccatccgttc gttcgcaagc aggagctgac ggtgtatacg ctgaataatg
     3901 agctggaaaa tctgctgacc aacgtggtga atcaggcgca acaggccggc aaagtgatgc
     3961 tcgacagcgt gccggtggac ccgaatatgc ttaaccagtt ccagagtacg atgccacagg
     4021 tgaaggagca gatgaaagcg gcggggaaag acccggtgct gctggtgccg ccgcaactgc
     4081 gtccattgct ggcgcgctat gcaaggttgt ttgcgccggg gctgcatgtg ctgtcgtata
     4141 acgaagtgcc ggatgagctg gagttgaaga ttatgggggc gttgatgtaa gaatccggca
     4201 tcggtgcaca attgccggat gcggcgtgaa cgccttatcc ggcctaccgt taaggcatag
     4261 atttgtaggc atgataagac gcgctagcgt cgcatcaggc atctgcgcac aaccgccgga
     4321 tgcggcgtga acgccttatc cggcctaccg ttaaggcaca gatttgtagg ccggataagc
     4381 gtaagcgcat ccggcaccgg cgcacaatta taaaataata cgctccccgg cactaaaatc
     4441 cgttacgtcc cccgcccaat cgatgggata taaacctttc gcgacatcgc gatggaacgt
     4501 tgagaatggc caatcactca cctgctttac ccagccatgt ttcactggat ttatgtaaat
     4561 ataatcaaca tgatgacgat aatctttggt attgcgaatg gcatgctccc aaaaacgcgg
     4621 ttgccacata ttttgcaatc cacaagcatg ggtgaactgt tttttaatct cccgccagcg
     4681 agaggaaaaa tcatcatcgc tttcgggtaa tgtccagata cagtgcatat gttccggcaa
     4741 aacgacccag gcgttaattt caaaaggtct ggcctgctta actttaataa tggcgttacg
     4801 gagtgtctgg tactgggtgg tcaaaagttg gcttcgacga ttccgtaaat ttaccgtgaa
     4861 aaaccatgtt ccccccttga tgtaataacg acgatattca gacatggcga ctcctttttc
     4921 tcaacgataa gaagaaaagg aaaagtcgac aatgaagttg tgttatatcg ggaaagcagc
     4981 tcgcaaaaaa ttcgcagact cgtaggcatg ataagacgcg gcagcgtcgc atcaggcatc
     5041 ggtgcactat tgccggatgc ggcgtgaacg ccttatccgt cctacaaaat cgtttcctcc
     5101 gtcaaaatcc tgcgcgcgcc taaaaaatgg tcctgccaga aaggttcggc taaccgactt
     5161 acccgaatgg tttccccggt gcgcggtgac tcgataaact gaccgtctcc aagatatacg
     5221 cccatatggt cggctatctc acgactgtgg atatggaaaa acagcaaatc tccccggcgc
     5281 aggtcgttat tcgccacaat cgttgcccga tggtagtgat acatctcatt ggccgtacgc
     5341 gggagcttcg cctcgaggat cttgttgtag gcataaaaaa ccaacccgct acagtcgaag
     5401 ccttgatcgg gccgcgtgcc gccccagacg taaggcttac caagctgctg ctccaggcgg
     5461 tgaatcgcca cttcggtgat gttgtgtaga tggtcgctgc ttaagaaatg gttgttctcc
     5521 gccagcgcct gccagcgtct gtcactcgcc ttgagcggcg caggaaacca ttctggatgc
     5581 tgttttatct gctcccgatt atgctggcgc agcgcccttc tgcttttggc attgccttcc
     5641 acaatatagc tggcctgctt tttcagatgg gtttggtact gttttaataa acgcgcacgg
     5701 ttttgcatcc gctggcgggc ggcgtagctg aaagagatat gtgatgtggt ggcgctggcg
     5761 agtaaaggga gcaaaacgca taacgaaaac acgcctggat gcagaaatct gccgtgcaag
     5821 aacgaagaca tgaaggacat aaaccagctg aatgaaggtg agaattttaa aagaccggag
     5881 gagtaaacgg gattattaat tgcttatttg catattacta ttgcggtttc ataatcttag
     5941 gaaataagaa ataaatctac aatccatgcg cttttcggac tcttgaaaac tgcccctgtg
     6001 ctaaatttgt aaaccctgcg ccgacaggct tgcgtaaaac tcttttggtg ataatgtgag
     6061 ccttccaaat ccagactatt ccgatttggg attcattttg ccgtcctggc gtgtgtaacc
     6121 gttttatcaa ggaataagca gtatgcgtaa aatcgcatta attcttgcga tgttgttgat
     6181 cccgtgcgtt tcgtttgccg gtttgctggg cagcagtagc tcgaccacgc cagtgagcaa
     6241 agagtataag cagcagttga tgggatcccc tgtctacatc cagatcttca aggaagaacg
     6301 tacgctcgat ctctacgtca aaatgggcga gcaatatcaa ctgctcgaca gctataaaat
     6361 ctgtaaatat tccggcggct tagggccaaa acagcgtcag ggcgatttca aaagcccgga
     6421 agggttttat agcgtccagc gtaatcagtt aaaaccagac agccgttact acaaagcgat
     6481 taatattgga ttcccaaacg cctatgaccg tgcgcatggt tatgaaggga aatacctgat
     6541 gattcacggc gattgtgttt ccatcggctg ctacgcgatg accaatcagg gtattgatga
     6601 gatcttccag ttcgttactg gtgcgctggt gtttggtcag ccgagcgtgc aagtgagtat
     6661 ttacccgttc cgtatgaccg acgccaatat gaagcgccat aaatattcca actttaagga
     6721 cttctgggag caactgaagc caggctacga ctactttgag caaacccgta agccaccgac
     6781 cgtttctgtg gtcaatggcc gttacgtggt cagcaagccg ttaagccacg aagttgtgca
     6841 gccacaactg gcatcaaact acacgctccc cgaggcaaaa taagcgccat tcacctggca
     6901 taatcttttg ccaggtttca ttgcccgtca gcggctgtgt cgcaatcacc gtgaccacat
     6961 cattcggtgt ggtctgcgag ctgaagtcga tttccacatc ctgatccagc aacgttgcca
     7021 cgccaaacgg cgcgcggcgg gtgatccagt gtaaattagt cgagcaatac gccattacat
     7081 agcgcccgtc agaaagcagc atgttgaaaa cgcccttctg ccgcagttca tccgccagtg
     7141 aggcgatata tttaaacact gccgccatgt tgcccggtgt gcgcgggtaa cgctgcgtta
     7201 atttatgcag gagccagcaa aaggcttttt cgctatcggt ttcaccgacc gggcggaagt
     7261 tgccggtttc cagtgatttg tagcccgtca gttgtccatt atgggcataa gtccagttac
     7321 gcccccataa ctcgcgggta aacgggtgag tattttccag cgccacctcg ccccgattag
     7381 cctggcgaat atgagccacc accgaacagg attttatcgg gtagtcctgg acaagtttgg
     7441 cgatggggga attaaagctg ggttgcggat ctttaaatgt gcgacagcct ttaccttcgt
     7501 aaaaggtaat gccccagcca tctttatgtg gcccggttcc accaccacgc tgtacaagtc
     7561 cggtgaaact aaagcagata tcggtaggga cgttggcgct catcccgagc aattcgcaca
     7621 tgggtaacct ccacaacagc gggagcgcat ggcccccggc gaagattgat taaaaagtac
     7681 aaaggttcag gctgttaccc tcaccccggc cctctccctg aaagggagaa aagaccttac
     7741 ttaaccatct ctttttcaat taactggatc aggatatgga tcactttaat gtggatctcc
     7801 tgaatgcggt cggcataacc aaagtgcggt acgcgaattt cgatatccgc tgtgccagcc
     7861 attttgccgc cgtctttacc ggtcagggtg atcactttca tccccttctc acgcgccgcc
     7921 gcgatcgctt tgatcacgtt tgcagagtta ccggaggtgg agatccccag cagtacatcg
     7981 ccttcgcgac ctaccgcttc aacgtaacgg gagaaaatat cattgaaacc aaaatcgtta
     8041 ccgacgcagg aaatatggct gacgtcagaa atggcaatcg ccgggtagcc cggacggttt
     8101 tcacggtagc gaccggtcaa ctcttcggca aagtgcatag cgtcgcaatg ggagccgccg
     8161 ttgccgcagg aaagcacttt gccaccggct ttaaagctgt ctgctaacag gaccgccgcg
     8221 cgctgaatgg cgtgaatatt ggcgtcatct tttaaaaaat tagccagcgt ttccgccgct
     8281 tcgttcagtt cgttacgaat aagatcctgg tacatgagga tatccttcag cataaatgta
     8341 atagacaaaa tgcagtgtac cggataccgc caaaagcgag aagtacgggc aggtgctatg
     8401 accaggactt tttgacctga agtgcggata aaaacagcaa caatgtgagc tttgttgtaa
     8461 ttatattgta aacgtattgc taaatgtttt tacatccact acaaccatat catcacaagt
     8521 ggtcagacct cctacaagta aggggctttt cgttatgatg attttgagta ttctcgctac
     8581 ggttgtcctg ctcggcgcgt tgttctatca ccgcgtgagc ttatttatca gcagtctgat
     8641 tttgctcgcc tggacagccg cactcggcgt tgctggtctg tggtcggcgt gggtactggt
     8701 gcctctggcc attatcctcg tgccatttaa ctttgcgcct atgcgtaagt cgatgatttc
     8761 cgcgccggta tttcgcggtt tccgtaaggt gatgccgccg atgtcgcgca ctgagaaaga
     8821 agcgattgat gcgggcacca cctggtggga aggcgatttg ttccagggca agccggactg
     8881 gaaaaagctg cataactatc cgcagccgcg cctgaccgca gaagagcaag cgtttctcga
     8941 cggcccggta gaagaagcct gcaggatggc gaatgatttc cagattaccc atgagctggc
     9001 ggatttgccg ccggagctgt gggcgtatct taaagagcat cgcttcttcg cgatgatcat
     9061 caaaaaagag tacggcgggc tggagttctc ggcttatgcc cagtctcgcg tgctgcaaaa
     9121 actctccgga gtgagcggga tcctggcgat taccgtcggc gtgccaaact cattaggccc
     9181 gggtgagcta ctgcaacatt acggcactga cgagcagaaa gatcactatc tgccgcgtct
     9241 ggcgcgtggt caggagatcc cttgctttgc actgaccagc cccgaagcgg gttccgatgc
     9301 gggcgcgatt ccggataccg ggattgtttg catgggcgag tggcagggcc agcaggtgct
     9361 ggggatgcga ctgacctgga acaaacgcta cattacgctc gcgccgattg cgaccgtgct
     9421 tggtctggcg tttaaactct ccgacccgga aaaactgctg ggtggcgcag aagatttagg
     9481 tattacctgt gcgctgatcc cgaccaccac gccgggcgtg gaaattggtc gtcgccactt
     9541 cccgctgaac gtgccgttcc agaacggacc gacgcgcggt aaagatgtct tcgtaccgat
     9601 cgattacatt atcggcggac ccaaaatggc cgggcaaggc tggcggatgc tggtggagtg
     9661 cctctcggta ggccgcggta tcaccctgcc ttccaactca accggcggcg tgaaatcggt
     9721 agcgctggca acgggtgcgt atgcgcacat ccgccgtcag ttcaaaatct ctattggtaa
     9781 gatggaaggg attgaagagc cgctggcgcg tattgccgga aacgcttatg taatggatgc
     9841 tgcggcatcg ctgattacct acggcattat gctcggtgaa aaacctgccg tgctgtcggc
     9901 tatcgttaag tatcactgta cccaccgtgg gcagcagtcg attattgatg cgatggatat
     9961 cactggtggc aaaggcatta tgctcgggca aagtaacttc ctggcccgtg cttaccaggg
    10021 cgcaccgatt gccatcaccg ttgaaggggc taacattctg acccgcagca tgatgatctt
    10081 cggacaagga gcgattcgtt gccatccgta cgtgctggaa gagatggaag cggcgaagaa
    10141 caatgacgta aatgcgttcg ataaactgct gttcaaacat atcggtcacg tcggtagcaa
    10201 caaagttcgc agcttctggc tgggcctgac gcgcggttta accagcagca cgccaaccgg
    10261 cgatgccact aaacgctact atcagcacct gaaccgcctg agcgccaacc tcgccctgct
    10321 ttctgatgtc tcgatggcgg tgctgggcgg cagcctgaaa cgtcgtgagc gtatctcggc
    10381 acgtctgggg gatattttaa gtcagcttta cctcgcctct gccgttttga agcgttatga
    10441 cgacgaaggc cgcaacgaag ccgacctgcc gctggtgcac tggggcgtac aggatgcgct
    10501 gtatcaggca gaacaggcga tggacgattt actgcaaaac ttcccgaacc gcgtggttgc
    10561 cgggctgctg aatgtggtga tcttcccgac cggacgtcat tatctggcac cttctgacaa
    10621 gctggatcat aaggtggcga agattttaca agtgccgaac gccacccgtt cccgcattgg
    10681 tcgcggtcag tacctgacgc cgagcgagca taacccggtt ggtttactgg aagaggcgct
    10741 ggtggatgtg attgccgccg acccgattca tcagcggatc tgtaaagagc tgggtaaaaa
    10801 cctgccgttt acccgtctgg atgaactggc gcacaacgcg ctggcgaagg ggctgattga
    10861 taaagatgaa gccgctattc tggtgaaagc tgaagaaagc cgtctgcgca gtattaacgt
    10921 tgatgacttt gatccggaag agctggcgac gaagccggta aagttgccgg agaaagtgcg
    10981 gaaagttgaa gccgcgtaat tgaataacgg agccgaaagg ctccgtttct ttatccgcta
    11041 attatttaaa attaaagcca tccggatggt tttccaggct gccggtcaac gccgcgaaca
    11101 gcaccgtttt accatcaata gaaagcgcat cgttcacatt caaccaggtg agtttctctt
    11161 gcgacgtttt ctcatcaata gtcgagaaca gccccgtcat ctgattagat ttctcggacc
    11221 acatcacagc gatacgttgc gagccacagt catgcggttt gcacgcgctc atcacctgat
    11281 acgtctcatc tcccaatgtc acggtttgtg cgggagtata agtaccgcct ttcatcaccc
    11341 aggcaggcag cttatgccct tgcaccatct gattaaacgc cgctttggtg gtttcgccct
    11401 ttgcaagact gctaatggtt aaatcatcct gcgccattgc actggtggcg atgaccagag
    11461 cggcgactgt cgttattgcc ttaaacatca ttcctcccgg gcttatcctg cccatgtgat
    11521 taactcccta aagcctggcg ataacacgat aatttgcaaa tcagatattt ccaatatcag
    11581 catttgttat caatttattc tgcgctaagg caactgcctg ataaggtgtt agagttataa
    11641 aacccttcgc ataaaggagt tgtacaccgt gcctggtttg aagattacgc ttttgcagca
    11701 accactggtg tggatggatg gtcctgccaa cctgcgccat tttgatcgtc aactggaagg
    11761 tattaccggg cgcgatgtga tcgttctacc ggagatgttt accagcggct ttgccatgga
    11821 agcggcagct tcgtcgctag cacaagatga cgtagtgaac tggatgaccg ctaaagcgca
    11881 gcagtgcaat gcgctgattg caggcagtgt tgcattacaa acggagtctg gttcggttaa
    11941 ccgctttttg ctggttgagc cgggcggcac ggtacatttt tatgataagc gtcatctgtt
    12001 ccgcatggca gatgagcatc tgcattataa agcgggcaat gagcgagtga ttgtggaatg
    12061 gcgcggctgg cgtattttgc cgctggtgtg ctacgactta cgttttccgg tgtggtcgcg
    12121 caatctcaac gattatgacc tcgccctgta cgtcgccaac tggcctgctc cgcgctctct
    12181 gcactggcag gcattgctga cagcccgcgc gattgagaat caagcgtatg tggcgggatg
    12241 caatcgcgtc ggcagcgatg gcaacggctg ccattatcgc ggtgacagcc gggtgattaa
    12301 tccgcaaggc gagattatcg ctactgccga cgcgcatcag gcaacgcgca ttgatgcgga
    12361 gctgtcgatg atggcattgc gggaatatag agaaaagttt ccggcatggc gggatgcgga
    12421 tgagtttagg ttgtggtgag tgggggttgt gtttagggct ggggagagtc agggtaagac
    12481 tacgaaagcc cgctc
//
LOCUS       DAZUCC010000149        12471 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZUCC010000149 DAZUCC010000000
VERSION     DAZUCC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.150"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..1537
                     /gene="caiT"
                     /locus_tag="ACHY09_003561"
     CDS             23..1537
                     /gene="caiT"
                     /locus_tag="ACHY09_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFS3886486.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1568..2710
                     /gene="caiA"
                     /locus_tag="ACHY09_003562"
     CDS             1568..2710
                     /gene="caiA"
                     /locus_tag="ACHY09_003562"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFS3886487.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2838..4055
                     /gene="caiB"
                     /locus_tag="ACHY09_003563"
     CDS             2838..4055
                     /gene="caiB"
                     /locus_tag="ACHY09_003563"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFS3886488.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4128..5681
                     /gene="caiC"
                     /locus_tag="ACHY09_003564"
     CDS             4128..5681
                     /gene="caiC"
                     /locus_tag="ACHY09_003564"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFS3886489.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQRKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5790..6575
                     /gene="caiD"
                     /locus_tag="ACHY09_003565"
     CDS             5790..6575
                     /gene="caiD"
                     /locus_tag="ACHY09_003565"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFS3886490.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEAQRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6581..7171
                     /gene="caiE"
                     /locus_tag="ACHY09_003566"
     CDS             6581..7171
                     /gene="caiE"
                     /locus_tag="ACHY09_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFS3886491.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAIIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYK
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7290..7685)
                     /gene="caiF"
                     /locus_tag="ACHY09_003567"
     CDS             complement(7290..7685)
                     /gene="caiF"
                     /locus_tag="ACHY09_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFS3886492.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7946..11167)
                     /gene="carB"
                     /locus_tag="ACHY09_003568"
     CDS             complement(7946..11167)
                     /gene="carB"
                     /locus_tag="ACHY09_003568"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS3886493.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11185..12333)
                     /gene="carA"
                     /locus_tag="ACHY09_003569"
     CDS             complement(11185..12333)
                     /gene="carA"
                     /locus_tag="ACHY09_003569"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFS3886494.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2888 a         3264 c         3323 g         2996 t
ORIGIN      
        1 gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa tagaaccgaa
       61 ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag tcagagatct
      121 ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg tatggggatg
      181 ggcatttgaa tggtatatgg tggtgatgct tttcggctgg ttctggctgg tgtttggccc
      241 gtatgccaaa aagcgtttag gtaacgaacc accagaattt agtaccgcca gttggatctt
      301 tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga ttgagatcta
      361 ctactacatc tccaccccgc cgtttggctt agaaccgaac tcaacaggcg cgaaagagtt
      421 ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt acagcttcct
      481 ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc gccccagctc
      541 caccctggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca ctatcgtcga
      601 caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc ttgccacgcc
      661 gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc aactggacgc
      721 tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg gtctgcaaaa
      781 aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc tgggttgggt
      841 gttcatcgtc agcggtgcca gcttcatcat gaactacttc accgattcgg tggggatgtt
      901 gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag gcggcttccc
      961 gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgctatcc agatgagcat
     1021 cttcctcgcc cgcatctccc gtggtcgtac cgtgcgtgaa ctgtgcttcg gcatggtgct
     1081 ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca ctctgctgtt
     1141 gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg tggcgcgcgc
     1201 catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg gcttcttcat
     1261 cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc tggcgatgtc
     1321 cacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta tcggctggtc
     1381 aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga aaccgattca
     1441 aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg tgacgctctc
     1501 ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa tatcaagagg
     1561 ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg gtatccgcga
     1621 actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg acagcgtcta
     1681 cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc tgattcctga
     1741 agagcacggt ggtcttgacg cgggttttgt tactctcgcc gccgtgtgga tggagctggg
     1801 acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca ataccttcct
     1861 gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca ccggtaagca
     1921 gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta gcctgaaaac
     1981 gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt ttattaccag
     2041 cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc cggacaaacc
     2101 tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga ccaaacttga
     2161 gaagctcggt ctgcgtatgg atagctgctg tgaaatcact tttgacgacg tggaactgga
     2221 cgagaaagac atgttcggtc gggaaggtaa cggatttaac cgcgtcaaag aagagttcga
     2281 ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg cctttgaaga
     2341 tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc gtttccagtt
     2401 gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa acatgctgta
     2461 tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag cgatgtgcaa
     2521 atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg tgctgggcgg
     2581 tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc gtgtagaccg
     2641 cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg tgctgaagca
     2701 ataccgttaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca ggcctacggg
     2761 agcacgaaga tggccggata aggcgtttac gccgcatccg gcagtcatgc gctacataaa
     2821 tttttcagga gctaataatg gatcatctac ccatgccgaa attcgggccg ctggccggat
     2881 tacgcgttgt cttctccggt atcgaaatcg ccgggccgtt cgccggacaa atgttcgcag
     2941 aatggggggc ggaagttatc tggatcgaga acgtcgcctg ggccgacacc attcgcgttc
     3001 aaccgaacta cccgcagctc tcccgccgca atttgcatgc gctgtcgtta aatattttca
     3061 aagatgaagg gcgcgaagcg tttctgaaat taatggaaac caccgatatc ttcatcgaag
     3121 ccagtaaagg tccggccttt gcccgtcgtg gcattaccga tgaagtcctg tggcagcaca
     3181 acccaaaact ggttatcgct cacctttccg gttttggtca gtatggcacc gaggagtaca
     3241 ccaatcttcc ggcctataac actatcgccc aggccttcag tggttacctg attcagaacg
     3301 gtgatgttga ccagccaatg cctgccttcc cgtataccgc cgattacttt tctggcctga
     3361 ccgccaccac ggcggcgctt gcagcactgc ataaagtgcg tgaaaccggc aaaggcgaaa
     3421 gcatcgacat cgccatgtat gaagtgatgc tgcgtatggg ccagtacttc atgatggatt
     3481 acttcaacgg cggcgaaatg tgcccgcgca tgaccaaagg taaagatccc tactacgccg
     3541 gttgcggtct gtataaatgc gccgacggct acatcgtgat ggaactggtg ggcattactc
     3601 aaattgcaga gtgctttaaa gatattggcc tcgcacacct gctcggtacg ccggaaatcc
     3661 cggaaggcac tcagcttatc caccgtatcg aatgccctta cggcccactg gtagaagaga
     3721 aactcgatgc ctggctggcg gcacatacca tcgcagaagt taaagaacgc tttgccgaac
     3781 tgaatatcgc ctgtgccaaa gtactgaccg taccggaact ggaaagcaat ccacagtatg
     3841 tggctcgcga atcaatcact cagtggcaaa cgatggatgg tcgcacctgc aaagggccga
     3901 atatcatgcc gaaattcaaa aataaccccg gacaaatctg gcgcggaatg ccctcacatg
     3961 gcatggacac ggctgctatt ttgaaaaata tcggctacag cgaaaacgac attcaggagt
     4021 tggtcagcaa aggtctggcc aaagttgagg actaaacgcg tggcgtcagt cagcttgcct
     4081 gactagcgcc cgctcaataa caaacgaaca aaatggatag aggtgcaatg gatatcattg
     4141 gcggacaaca tctacgtcaa atgtgggacg atctggcgga cgtttacggt cataaaacgg
     4201 cgctgatttg tgaatccagc agcggagtcg ttaaccggta tagttatctt gagttaaatc
     4261 aggagattaa ccgcacggca aacctgtttt atgtgctggg gattcgcaaa ggcgacaagg
     4321 ttgctctaca tctcgacaac tgcccggaat ttatcttttg ctggttcggg ttggcaaaaa
     4381 ttggcgcgat tatggtgccg attaacgccc gcctgttgcg cgaagaaagc acgtggatcc
     4441 tgcaaaatag ccaggcgtgc ctgctggtga ccagtgcgca attttatcct atgtatcaac
     4501 agattcagca ggaagatgcc tctcagttgc ggcacatttg cctgacagat atggtacttc
     4561 ccgctgatga tggcgtgagt tcgtttactc aacggaaaaa tcaacaacct gccaccttgt
     4621 gctatgcacc gccgctatcg actgacgata cggcggaaat tctcttcacc tccggcacca
     4681 cctcccgacc gaaaggggtg gtgataactc attacaacct gcgcttcgct ggatattact
     4741 ccgcctggca gtgtgcactg cgtgacgatg acgtctacct gacggtaatg cctgcgtttc
     4801 atatcgattg ccagtgtact gcggcgatgg cggcgttttc tgccggggcc acctttgtgc
     4861 tggtcgagaa atacagcgcc cgcgccttct ggggacaggt gcagaagtac cgcgccacca
     4921 ttaccgaatg tattccgatg atgattcgta cgttgatggt gcagccgcct tcagccaacg
     4981 atcggcaaca ctgcctgcgg gaagtgatgt tttatctcaa cctgtcggag caggaaaaag
     5041 acgcgttttg tgaacgcttc agcgttcgct tgctgacgtc ttatgggatg acggaaacca
     5101 ttgtgggcat tatcggcgat cgccctggcg ataaacggcg ctggccgtcg attggtcggg
     5161 cgggcttttg ctacgaagcg gagatccgcg acgatcacaa tcgtcccctc ccggctggtg
     5221 agctcggtga aatctgtatt aaaggcgtac cagggaaaac catcttcaaa gagtattttc
     5281 tcaacccgaa agccactgcg aaagtgctgg aagccgatgg ctggctacat accggcgata
     5341 ccggataccg cgacgaagag ggcttttttt atttcgtcga tcgccgctgc aatatgatca
     5401 aacgcggtgg cgagaatgtc tcctgcgtgg agctcgaaaa tatcatcgcc acccatccaa
     5461 aaattcagga tatcgtggtt gtgggtatta aagattcgat tcgcgatgaa gccatcaaag
     5521 catttgtggt gctgaatgaa ggtgaaacat tgagcgaaga ggaattcttc tgcttctgtg
     5581 aacagaatat ggcgaaattt aaagtgccct cttatctgga gatcagaaaa gatctgccac
     5641 gtaattgctc ggggaaaata attagaaaga atctgaaata aatgaaacgg cagggaacta
     5701 ccctgtcagc taataatcac gctctaaaac aatatgcatt ttttgcaggg atgttgtcgt
     5761 ccctgaaaaa gcaaaaatgg agaaaaggaa tgagtgaatc attacatctg acccgcaatg
     5821 gatcaattct ggaaattacc cttgatcgtc caaaagcgaa tgctattgat gcaaaaacca
     5881 gctttgaaat gggcgaagta tttctaaatt tccgtgacga tccgcaatta cgtgtcgcca
     5941 ttattaccgg tgccggagag aaattctttt ccgcgggctg ggatttaaaa gcggcagcag
     6001 aaggcgaagc accggatgct gactttggcc cgggaggttt tgcgggatta accgaaattt
     6061 tcaatctcga caaaccggtt atcgcagctg tgaacggcta cgcctttggc ggcggtttcg
     6121 aactggcgct ggcggcagat tttattgttt gtgccgataa tgccagcttc gccctgccgg
     6181 aagccaaact gggcatcgtt cctgacagcg gcggcgtgct gcgtctgccg aagatcctgc
     6241 cgcctgccat cgtcaatgaa atggtgatga ccggcagacg aatgggcgca gaagaggcgc
     6301 agcgttgggg gatagtcaac cgcgtggtta gccaggcgga actgatggat aacgcccgcg
     6361 aactggcgca gcagctggtt aacagcgctc cactggcgat tgcggcgctg aaagagatct
     6421 accgcaccac cagcgaaatg ccggtagaag aagcgtatcg ctatattcgc agcggcgtgt
     6481 tgaaacacta tccatcggtt ctgcattcgg aagatgccat tgaagggccg ctggcgtttg
     6541 ccgagaagcg cgatccggtg tggaaaggac gttaacaacc gtgagctatt acgcctttga
     6601 gggattaatt ccggtggttc acccgacggc gtttgtccat cccagtgcag tattgattgg
     6661 cgatgtgatt gtgggagccg gtgtctacat cggcccactc gcctcactgc gtggtgacta
     6721 cgggcggttg atcgtgcaag cgggagccaa tattcaggat ggctgcatta tgcatggcta
     6781 ctgcgacact gacaccatcg ttggggaaaa cggccatatc gggcacggag cgatcctgca
     6841 tggttgtgtg attggtcgcg atgcactggt cgggatgaac agcgtgatta tggatggcgc
     6901 gatcattggc gaagagagca ttgttgccgc tatgagcttt gtcaaagcgg gctttcgcgg
     6961 cgagaaacgc cagttgttga tgggtacgcc agcccgcgct gtacgcagcg tgagtgacga
     7021 cgagttacac tggaaacggt tgaataccaa agagtataag gatcttgtcg ggcgctgtca
     7081 tgcatcatta catgaaacgc agccactgag gcaaatggag gaaaatcgcc cccgtttgca
     7141 ggggacgacg gatgtgacgc cgaaacggtg atctttccgg atgcggcgta aacgccttat
     7201 ccgacctggc tggaaaggaa ccgtaggccg tataagatgc gccagcatcg catccggcga
     7261 ccaggtcgca cggtatcaac gaaacgacat taacgacgca tactctttga caacatcatc
     7321 tgccacttct gctcgcggtt aagttccgtg agcggaacgg caggaatacg tggcgtcttt
     7381 cttacgccga ctaatttttc ccgactgtta ttgcgcaggc gcgcgtaaat ttgctcatcg
     7441 atatcgacca ctttaaccag tcgctgacac tggcatcccc gcccttccag cttattaggg
     7501 atcatcttaa cttcgcagct tatttctgtg acttccgaca gaatataagt cagggtatta
     7561 accgccttgc tgtgttcaat atcgaaatga atagagatct ctcttgctat cacccaccga
     7621 ttttcagcca tcatccattc ggcgattaac aagtagagtg gtttttcaac atatccttca
     7681 cacatacgaa ctccagtttc acaaattaaa agtctggcta tgactcgttt tgcagcaatt
     7741 aaacaaggcc atattaaaat tgtggatata tcgatagtat gcaacttcac cgtcgataag
     7801 tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat
     7861 gggcaaaaaa aacggtcctc atcagagaac cgtattagtt attcgatcaa attagcggat
     7921 gaaaaatatc cgccatgaca cgctattatt tgatctgcgc gtgcatttcc tgcaccgaaa
     7981 ttactttttc agtcgcatcg gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg
     8041 tggtgtcgta atgcacttta tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa
     8101 tcgcacgacg acctgaggtg gtgttgatga tgtaggtata ttcgccattc ttgatacggt
     8161 cctgaatgtg cggacgacct tcatgcacct tgttcaccag acgcggatta atgcctgctt
     8221 cgcccagcac aatcgccgtg ccgtgggtcg catccagctc gaagccctgt ttcagcagtt
     8281 ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc aacgcacgac
     8341 cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg
     8401 tgcggcccac gcccatgact tccccggtag agcgcatttc tggccctaac agcgggtcaa
     8461 cgcccgggaa tttattgaac ggcagcacca cttctttcac cgagtagtac ggcgggataa
     8521 cttctttggt tacgccctgc tcagccagcg atttgccagc catcacacgc gccgccactt
     8581 ttgccagcgg tacgccggtg gctttggaga cgaacggaac ggtacgcgcc gcacgcgggt
     8641 taacttcaat caggtagact tcgttgtttt tcaccgcaaa ctgcacgttc atcaggccgc
     8701 gcacctgcaa ttcgaaggcc agtttctgca cctgctggcg catcacatcc tgaatttcct
     8761 gacttaaagt gtaggctggt agcgaacatg cagagtcacc ggagtgcacg cccgcctgct
     8821 cgatgtgctc catgatgccg ccaatcagca ccatttcacc gtcgcagatg gcatccacgt
     8881 caacttctac tgcgtcatca aggaagtggt ccagcaacac tggcgcatcg ttagatacgc
     8941 tgaccgctgt ctggaagtag cgacgcaggt cagcttcgtc atagacgatt tccatcgccc
     9001 gaccgccgag aacgtaagac ggacgcacca ccagcgggta gccaatctct ttcgctttct
     9061 caaccgccat ttcgatggtg gtaacggtgg cgttcgccgg ttgtttcagt ttcagacgct
     9121 caaccgcatg ctggaagcgt tcacggtctt ccgcacggtc gatggcatcc gggctggtac
     9181 cgataaccgg tacgccagca gcttccagcg cgcgcgccag tttcagcggg gtctgaccgc
     9241 cgtactggac gataacgcct ttcggcttct caatacgcac aatttccagc acgtcttcca
     9301 gagttaccgg ctcgaagtag agacggtcag aggtgtcgta gtcggtggag acggtttccg
     9361 ggttacagtt aaccataatg gtttcataac catcttcgcg cagcgccagc gaggcgtgta
     9421 cacagcagta gtcgaattcg ataccctgac cgatacggtt cgggccgccg ccgagcacca
     9481 tgattttttc acggtcggta gacggattcg cttcgcactc ttcttcataa gtggagtaca
     9541 tgtaagcggt gtcggtggcg aactctgccg cacaggtatc cacgcgctta taaaccgggt
     9601 gcaagtcata ctggtcgcgc agcttgcgga tttctgcttc acgcacgccc gcgagttttg
     9661 ccagacgcgc gtcggcaaag cctttgcgtt tcagctggcg caggaagtca gcgttcaggc
     9721 cagtgatgcc cacttctgcg actttctctt ccagacgcac cagctcttca atttgtacca
     9781 ggaaccagcg gtcaatgttg gtcaggttga acacgccgtc tacggacagg cccgcgcgga
     9841 aagcatcggc gatataccag atacgctctg cgcctgcatc tttcagttcg cggcgaattt
     9901 tggtcagcgc ttccggatca tcgaggctga ctttcgggtc gaagccagtt gcgcccactt
     9961 ccaggccgcg cagcgctttt tgcagggatt cctgctgcgt gcgaccaatc gccatcactt
    10021 cgccaaccga tttcatctga gtggtcagac ggtcgttcgc cccggcgaat ttctcgaagt
    10081 tgaagcgagg aattttagtc accacatagt cgatggacgg ctcgaaggag gccggagtac
    10141 gcccaccggt gatgtcgttc atcagctcgt cgagggtgta acccaccgcc agtttcgccg
    10201 ccactttggc aatcgggaaa ccagtggctt tcgacgccag cgcagaggag cgagacacgc
    10261 gcgggttcat ttcgataaca atcaggcgac cgtttttcgg gttcaccgca aactggacgt
    10321 tggaaccacc ggtttcaacg ccgatttcac gcagtaccgc catcgaggcg ttacgcatga
    10381 tttgatattc tttgtcggtc agcgtttggg ctggcgcgac agtgatggag tcaccggtgt
    10441 ggatgcccat cgcatcaaag ttttcgatag agcagacaat gatgcagttg tcgtttttat
    10501 cacgcaccac ttccatctcg tactctttcc agccgatcag cgactcatca atcagcagct
    10561 ctttagtcgg agagagatcc agaccacggg cgcaaatttc ttcaaactct tcgcggttat
    10621 aagcgatacc gccgccgcta ccgcccatgg taaaggatgg gcgaataatg cacgggaagc
    10681 caacttcagc ggcaaccgcc agcgcttctt ccattgaatg agcgataccg gaacgcgcgg
    10741 tttccagacc gattttcttc atcgctacgt cgaaacggcg gcggtcttcg gctttatcaa
    10801 tcgcatcggc agtggcacca atcatggtga cgccgaactc ttccagcacg ccctgacgct
    10861 caagctcaag cgcacagttc agcgccgtct gcccccccat ggttggcagc accgcgtccg
    10921 gacgctcttt ttcaataatc ttgcgtacca cttcccagtg aatcggttcg atgtaggttg
    10981 catcagccat ttccgggtcg gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc
    11041 ggtaaccctc ttcacgcagg gctttacacg cttgcgcgcc agagtagtca aactcacacg
    11101 cctgaccgat aacaatcggg cccgcaccca gaatcaggat actttttata tctgtacgtt
    11161 ttggcatggc tcttttactc ctgattactt agcggtttta cggtactgct caattaactc
    11221 gataaagtgg tcaaacaacg gcgcggcgtc gtgtggacca gggctggctt ccgggtgtcc
    11281 ctggaagctg aacgccggtt tatcggtacg atgaatgccc tgtaacgtac cgtcgaacag
    11341 ggatttatgt gtgacacgca ggtttgcagg taatgttgct tcatccaccg caaaaccgtg
    11401 gttctgggcg gtaatcatca ccacattttt ctcaacatct ttaaccggat ggttgccgcc
    11461 gtggtgacca aatttcattt ttacagtctt cgcaccgctc gccagcgcca gcagctgatg
    11521 accgaggcag atgccgaata ccggaatatc ggtttcgagg aatttctgga tggcggtaat
    11581 ggcgtaatcg cacggggccg ggtcgccagg accgttggag aggaagatgc cgtctggatt
    11641 cattttcagc acgtcttccg cagaagtttg cgccggaacg atggtcagac gacagcctct
    11701 atccaccaac atgcgcagga tgttgcgctt ggcaccaaaa tcataagcca caacatggaa
    11761 cggcagctcg tcttctttct tcgcttctgg caggccgccg gtcaacgtcc agctcccttg
    11821 tgtccagcta taggcttctg cggtggtcac ttcttttgcc agatccatgc cgttcagacc
    11881 tgggaacgcg cgggcttttt ctaatgccag cgccgcatcc gggttatcgc ctgcgataat
    11941 gcagccattc tgtgcgcctt tctcgcgcag taaacgcgtc agcttacggg tatcgatatc
    12001 ggcaatcgcc acgatgttat ggcgcttcag gtaagaagag aggtcttcgg tattacggaa
    12061 gttgctggca atcagcggca ggtcgcgaat caccagacct tgtgcatgta cctgagaaga
    12121 ttcttcatcg gcgtcattag tgccgacatt accaatatgg ggataagtaa gagtaacgat
    12181 ttgacgagaa taggaaggat cagtgaggat ttcttgataa ccggtcattg aagtattgaa
    12241 aacgacttcc ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa actgggttcc
    12301 gtcttccaga accaatagcg ctgacttaat caaaacaccc tccagagaat attcactcac
    12361 tttatttgca tattaattca caatgatgac gtaaatcaat gcaaatctgc ttaccgacga
    12421 atttctggca aacggcggca ttctggagat atgggcgcta aaagtcaact t
//
LOCUS       DAZUCC010000150        12454 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZUCC010000150 DAZUCC010000000
VERSION     DAZUCC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.151"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(115..888)
                     /gene="uxuR"
                     /locus_tag="ACHY09_003570"
     CDS             complement(115..888)
                     /gene="uxuR"
                     /locus_tag="ACHY09_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFS3886495.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1103..2563)
                     /locus_tag="ACHY09_003571"
     CDS             complement(1103..2563)
                     /locus_tag="ACHY09_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFS3886496.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2644..3828)
                     /gene="uxuA"
                     /locus_tag="ACHY09_003572"
     CDS             complement(2644..3828)
                     /gene="uxuA"
                     /locus_tag="ACHY09_003572"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS3886497.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIVGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4168..5511
                     /gene="gntP"
                     /locus_tag="ACHY09_003573"
     CDS             4168..5511
                     /gene="gntP"
                     /locus_tag="ACHY09_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFS3886498.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5684..6586)
                     /gene="fimH"
                     /locus_tag="ACHY09_003574"
     CDS             complement(5684..6586)
                     /gene="fimH"
                     /locus_tag="ACHY09_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFS3886499.1"
                     /translation="MKRVIYLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6606..7109)
                     /gene="fimG"
                     /locus_tag="ACHY09_003575"
     CDS             complement(6606..7109)
                     /gene="fimG"
                     /locus_tag="ACHY09_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFS3886500.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTAGFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7122..7652)
                     /gene="fimF"
                     /locus_tag="ACHY09_003576"
     CDS             complement(7122..7652)
                     /gene="fimF"
                     /locus_tag="ACHY09_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFS3886501.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAVKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7662..10298)
                     /gene="fimD"
                     /locus_tag="ACHY09_003577"
     CDS             complement(7662..10298)
                     /gene="fimD"
                     /locus_tag="ACHY09_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFS3886502.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10365..11090)
                     /gene="fimC"
                     /locus_tag="ACHY09_003578"
     CDS             complement(10365..11090)
                     /gene="fimC"
                     /locus_tag="ACHY09_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFS3886503.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11127..11666)
                     /locus_tag="ACHY09_003579"
     CDS             complement(11127..11666)
                     /locus_tag="ACHY09_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3886504.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     VEAGDKQMTVNMGKISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11731..12285)
                     /gene="fimA"
                     /locus_tag="ACHY09_003580"
     CDS             complement(11731..12285)
                     /gene="fimA"
                     /locus_tag="ACHY09_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFS3886505.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTA
                     IDGTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         2958 a         3280 c         3036 g         3180 t
ORIGIN      
        1 aatatatcgt acgtaatgat taatttacgc ataatatatt ctgcagaaca taattaacta
       61 ttcagggaac atacatagtg caaagcggtt caactttgca ccagaaatga gggatcaggc
      121 gtcgacttta tccagcggcc atgaatcaaa cagatagccg tcaaaataaa tatcgtcaac
      181 gttcgagaat tccagcagac gttgtttaac gttttccaga tgctgccaca ttgccagctt
      241 cgccgcacgg gcatcttttt tgattaacgc ggcgaggatc tgtttgtgat cgcccaacca
      301 ctctttgcga tacaggctgt catcgagatg gctgtgcaat tggatccaca ttggattgtt
      361 ttcccgccac tgccaggact gacggaataa ctccaccagc atgctgttat gcgttgcttc
      421 ggcaatggcg agatggaact gcatatcacc gctttcgctg ctacccggcg cgctggaagc
      481 cagttcacgc tcttccagtt gcaatgcctg acgcattttg acgatatctt cgcgggtagc
      541 ctgcaaagcg gcaaattccg cgatgttact ttccagtaac tgacgcgcct gtaacagctc
      601 aaaaggaccg gcatcattac agacgttggc atccggactg tctgtgttgt ggctgcctga
      661 gctatcaaga acataaatac cggcaccccg gcgcacttcc accagccctt tgatctccag
      721 catgatcagc gcttcacgta ccaccgtccg cgtgacatca agcatttctg caatttcacg
      781 ctccggtggc agccgttcgc caggattgta cggcgtcttt atgatcagat cgcggatcat
      841 cgccccgact tcctggtaag gtctttgcgc agaggtggca gatttcatgg tgttgtgtcc
      901 gttaggtgcg gttaatcaaa ttttttccgg atgcggcgtg aacgccttat ccgacctaca
      961 acgggcaatt ttacggcacc cgttaagctc aaactatacc attagcactg gatgttggca
     1021 cgtacaggtg ggcaaaaaaa cgaaaactgg tcaaccaaat attagtttga ttgaccagta
     1081 agtctgtagg gtaatcagtt agttacagcg cagccacaca ctcgcgcgca ccgcgctcac
     1141 acagctgctg atatgccgcc gtcaccgcgc caacaaagtc ggcgttctgc ggcagatcat
     1201 cggcaaagat accgctcagg cccagcagtg ctttcacgcg gtctgcaccc tgatactgcg
     1261 cgttgatctt ctggaactcc gccagcatcg ggtcaaccac gtcaatggca ttaccctgct
     1321 catccacgcc ctgggtgtaa cgcatccagc ccgccacgcc cagcgccagg tgacgccagc
     1381 taccgccgtt ttgcaggtgc agacgcaccg ggtccagcag acgctgcggt aacttctggc
     1441 tgccgtccat tgcaatctgc caggtacggt gacgcagaga cgggttgctg aaacgctcga
     1501 tcagcagcgt cgcataagcg ttcaggtctg taccttccgg catcgacagc gttggcgctt
     1561 gttcctgcat catcagggca aaggccgctt tgcgataagc cgggttagtc atggtgtcgg
     1621 caatagtttc atagccgccg aggtaaccga ggtacgccag gaaagagtgg ctaccgttca
     1681 gcatacgcag cttcatcatt tcgaacggta caacatccgc aacaaactgc gcgccgactt
     1741 tatcccagtc cggacgacca ttaacgaagt tatcttcaat tacccactga cggaacggtt
     1801 cgcaggcaat ggcgcacggg tcgtaaacac ccagctggtc agcaatttcc tgtaaggttt
     1861 ctggcgtcgc cgccggaacg atgcggtcaa ccatggtgca cgggaaggtg acgttctctt
     1921 caatccatgc cgccagttgc gggtcacgcg cctgagccag cccaagaacg gcaaccttcg
     1981 ccacatgacc gttttcacgc acgttatcgc aggacatcac cgtaaatgct ttcagccctt
     2041 tttcacgacg cagacgcagg gcttctacga tgtaaccaat cgcagacttc ggcgcagtcg
     2101 ggttttccag atcgtgcttg atcagcgggt tattgagatc cagttgaccg cttgccgcat
     2161 ctgcgcagta gcctttttcc gtgaccgtca gagagacaat cgccgtctgc ggacgcgcca
     2221 tcgcgttgag aataccttcg caaccgtcga tttccgggtg cagggcttct ttcatcgaac
     2281 cgataatttt cagctcggtg ctttctgcgc ctttttccgc cacggtgtac agcagttgct
     2341 gttttttcag gttttcgatc agcacgcggt cgttgcctgg catcaggtta acttcgcaga
     2401 tcccccagtc gctatccgtg ctttccagca ggtgatgagt gtacagcgcc tggtgcgcgc
     2461 ggtgaaacgc cccgcaaccg agatgcacaa tgcgtgattc cagacgagaa tgatcccatg
     2521 acgggcgggc aaccggcaga ttgctgtcaa caatagtagt catcgtaaac tccagaatcc
     2581 gtcttcagag agtgacggca aggaagaagg aaccggcacg gcgcgcagcc atgccggtgg
     2641 atattaacgg ctaaagaaag cgcgctggat cgccagttcg acaccgcgaa cttcggccag
     2701 gcctttcaga cgaccaattg cggagtaacc aggattggtt ttcttcttca ggtcgtccag
     2761 catctgatga ccgtggtccg gacgcatcgg gatcaggtct tctttgcctt ccgctttacg
     2821 acggtgttct tcttcaacaa tcgctttcac cacttcgtac atatcaacgt caccgttcag
     2881 gtgcgccgct tcgtggaagg ttttcgggtt atcttcacgc atggtggagc gcagatgggt
     2941 gaagtaaata cgcggaccga actgcttgat catatcaacc agatcgttgt cagcacgcac
     3001 gccgtaggaa ccggtgcaca tggtgaaacc gttcgccatg ctgtttacgg tatcaaccat
     3061 ccactgcata tcttcaatgg tggaaacaat gcgcggcagg ccgaggatcg ggcgcggcgg
     3121 atcgtccggg tgaaccgcca tacgcacgcc aacttcttca gcaactggaa taatcgcttt
     3181 caggaagaca gcgaagtttt cgcgcagctt cgctttgtcg atatctttgt acagctccag
     3241 gtgcttacgg aactggtcga gggtataccc ttcttccgca cctggcagac cgacaatgat
     3301 gttacgggtc agacgcgctt tgtcttcgtc gctcatagtg gcgaagcgaa cagctgcctg
     3361 agcaatttct tcttcggtgt aatccgcttc cgcgcctgga cgcttcagga tatgcatttc
     3421 gaatgcagcg aattcgatct ggtcgaagcg cagagctttg gagccgtctg gcagcacgta
     3481 ttcgaggtca gtacgggtcc agtcgagcac cggcatgaag ttgtagcaca cggtgcgaat
     3541 gccgcactgc gccaggttgc gcagggtctg ctgatagtta gcaatccact gctcatagtt
     3601 gccagtgtgg gttttgatat cttcgtgaat tggtacgctt tcaacgacag accacaccag
     3661 gcctgcgtct tcaacgatcg ccttgcgttt gaggatctct tctacggacc atacttcgcc
     3721 gttcgggata tggtgcagcg cggtaaccac gccagttgcg cccgcctgac ggacatcagc
     3781 taaagaaacc ggatcgtttg ggccgtacca gcgccaggtc tgttccatga tttttcctct
     3841 ttcacatagt gggttccaca gcaaagagac gtggagcccc ggagttgttt aggtatccgg
     3901 atgaacaacc agaccgcagc cgtggcgttg catcggatga gatggcgtat aagttctacc
     3961 ttttggtaag acatgtcaac ccgaaatctg aaattggtta accacatcac aagaatttca
     4021 ctctcctggt tgttgctgca aattgcagca aagggtaatc gatggttatt gatcagagga
     4081 tgtgacattc atcgcaacaa tggttgacca atttacataa catgtcgccc aaaataacaa
     4141 cggttcaacc aggtaaggta aaacgttatg catgtgctta acattctctg ggtggtattc
     4201 ggcattggtc tgatgctggt actgaatttg aagttcaaaa tcaattcaat ggtggctttg
     4261 ttggtggcgg cgctgtccgt cgggatgctg gcgggcatgg atttgatgtc gctgctgcac
     4321 accatgaaag cgggcttcgg caacacgctg ggggaactgg ctatcatcgt ggtgttcggt
     4381 gcggtcatcg gtaaattgat ggtcgactcc ggcgcggctc accagatagc acatacgctg
     4441 ctggcgcgtc tcggtctgcg ctatgtacag ctgtcggtga ttatcatcgg cctgattttc
     4501 ggtctggcga tgttttatga agtggccttt atcatgttag cgccgctggt tattgttatt
     4561 gccgccgaag ctaaaattcc gttcctgaaa ctggcgatcc cggcagtagc agctgccact
     4621 accgcacatt cactgttccc accgcagccg ggtccggtgg cactggtgaa tgcttatggc
     4681 gcggatatgg ggatggttta tatctatggc gtactggtga cgatcccaag tgtcatctgc
     4741 gcaggtctga tcctgccgaa gttcctcggc aatcttgagc gcccaacgcc atcattcctg
     4801 aaagcagatc aaccggtaga tatgaataat ctgccctctt tcggcgtttc gattctggtg
     4861 ccgctgatcc cggcgatcat tatgatctct accaccatcg ccaatatctg gctggtaaaa
     4921 gatacccctg cctgggaagt ggttaacttt atcggttcct cgccgattgc aatgtttatt
     4981 gcgatggtgg ttgcattcgt actctttggc accgcgcgtg gtcatgacat gcagtgggtg
     5041 atgaacgctt ttgaaagcgc ggtgaaaagc attgcaatgg tgattctgat catcggtgcg
     5101 ggtggcgtgc tgaagcagac catcatcgac accggcattg gcgacaccat cggcatgttg
     5161 atgtcccacg gcaatatctc gccctacatc atggcatggc tgatcaccgt actgattcgt
     5221 ctggcgacgg gtcagggtgt cgtttcggcg atgaccgctg ccgggattat cagtgctgca
     5281 atccttgatc cagcaaccgg tcagctggtt ggcgtgaatc cggcgctgct ggtgctggcg
     5341 acggctgcgg gttccaacac cctcacgcac attaacgatg catctttctg gttgttcaaa
     5401 ggttactttg acctgtcagt aaaagacacg ttgaaaacct ggggtctgct ggagctggtc
     5461 aactccgtgg ttgggctgat tattgtgttg attattagca tggtagcgta aaagaacacc
     5521 ggagcctgcc aatggcaggc tctgttcgga ttgtcggtaa agtgcgcttt gtttatgcca
     5581 gatgcgacgc tgacgcgtct tatctggcat acaaagggct aacgtgcagg ttttagcttc
     5641 aggtaatatt gcgtacctgc attagcaatg ccctgtgatt tctttattga taaacaaaag
     5701 taacgccaat aatcgattgc acattccctg cagtcacctg ccctccggta cgtgcgtaat
     5761 ttgccgttaa tcccagactt accgccgaag tccccactgc tcctaacgat accgtgttat
     5821 tcgctggaat aatcgtaccg ttgcgcgtca actgtacgcc gacgccctgt gctggtgaaa
     5881 acgaggcggt attggtgaaa atcgagttgc ccgcatctgc ggttgtgccg gagaggtaat
     5941 accccaggtt ttggcttttc gcacaataaa cggtaagagg aatcggcacc gaaccgggat
     6001 agtccggcag agtaacggtg acatcacgag cagaaacatc acagccgcca gtaggcacca
     6061 ccacatcatt attggcgtaa atattccaca caaactggaa atcatcgctg ttatagttgt
     6121 tggtctgtcg caaaattagc acggcaatta atgagccagc tttaatcgcc accccgcccg
     6181 cactgctcac aggcgtcaaa taaagcgcca ccggccacgg cttatccgtt ctcgaattat
     6241 aaacaacgcg cggcgtttcg ctagtggtcg gaaatggata gctactgcca ctatatttta
     6301 cggtcccgga aaaattagat aacacgccgc cataagccga gcctcgttgc agtgtgacat
     6361 agtctgtaat ggtttccgga taatcgttat ggcaaaagat ttgcgtcgaa agatccacga
     6421 ccaggttttg ccccacattc acggcaggcg caaggtttac ataaacatta gcgctgccac
     6481 cgccaatagg gatagcggtg ccattggcgg ttttacaggc gaatgaccag gcatttaccg
     6541 accagcccat cagcagtaca gcaaacaggt aaataactcg tttcattaca atcatctctt
     6601 cggcttcagc tgtaggtata ggtgatgcta attactgcct gaatggttcc ctgagtggct
     6661 ccgccattta ccgtcaatgc tctgacctgt aacgggaagt gcgctgattg tgaggaatca
     6721 tccacctgaa ctgttttggt tgcgccacta ttcaatgtgt tgccactgtc atcctgtagc
     6781 tctaactgga tgttttgcgc ggtcccctgg tttttataat atccggtact gtcggctgcc
     6841 ccgctgaagc cggcggtgac cctcgacgtt cccaccggac aattagtcaa ctcaagcgca
     6901 acatcatgcc aggccgatgc cgccccggca gacatcagac tgaaagaata aagatcgccg
     6961 agatctaccg tggcattggt ggtggaaacc gtgcacggtt tggcgacgac cttaccgttc
     7021 accgtgatgg tgacatcggc tgcctgtatc gtcgcacttg cgagcgccaa cattgccgcc
     7081 aataaatacc cacgtttgcg ccatttcatg agcatctcca gttactgata ttcaagagtg
     7141 aaggtagccg tggcattgat atgccccgca gtgacaggca cctgtgtcgc cattagccgg
     7201 gcgtaaaaat tcagcgtatt tggtttaccc ggcgtcaggg tcgtccacga aatcgcggac
     7261 gatggggcat taaggggtat ctgattttgt tgctcattca gaagctgtat tcccagtccc
     7321 gcagccgctg acaccgtatt ttcaagtgca agcaggttgg cattgtggct atctgcaaca
     7381 ccggtaaacc caacttttac ggcagaaacg gcattaccac agggtgacag caaaatacga
     7441 aatggaacga caggagtcgt cgcgccaatg ttgttaaatt gcttcaccgc gttttccatc
     7501 agatcaacag taaaattggt tgattcagcg gccacactac agccattatc tctgacatag
     7561 ccgcggatag taatcgtgct atccgcagcc aaagcgtgac ttaccgccag ccacaaaaaa
     7621 gcgcaaagaa gataaaaagg tttgtttctc atcacgcctc cttaacgaca ttcagctgat
     7681 agctgggtta ataactgctg ctgactctct ggtggcagtt gataattggc gacacagtga
     7741 gcattttcct cttctcccca tttcacctga acttttcccg ctaaaggcat tccgctgagg
     7801 taaacctgac cattatccgc aacaatgccg ctactctggc tactctctga tgtcaccatc
     7861 gccccaaacg gcagcggctt attattgtgg gttagcgtca tgagcagttt tatcccaacg
     7921 cgcgctttaa actctgctcg cacgatcgcc ccacgggtgg gaacaacgtt agcgaccgcg
     7981 ttatctaaat cgacgttatc agccagggta ttggtatcca gcgctactct gttttcccga
     8041 tattcagtgg cataaggcag cacggcataa ccgcgccagt cggtacgcac ccccgtctgg
     8101 ttttcgactt ttgcatcttt tgcgccaggc gctttaacaa gcaccaccgt atcgtttaac
     8161 ggctgcccca gcgttacgcc attggcatga gccagtaccc caccgctgac tccgtaatag
     8221 agctgcttaa tatcatcgct atggctgtaa ccgatgttgg cattgccgta accaccgcga
     8281 taattcagcg tggcgtagcc tgtacttccg ctattaccat cacctccccc ggcgtaaccg
     8341 gtttgcacgc tatagctgag gttgttgtct tccagcaacg taccgtatac accggccaga
     8401 ttggtcatcc gaccgttgag atcgtgtgac atgctgtaac tggcactggc atgtcgccac
     8461 tgagatttac tgtcagaacg cagccagtgg ctgaaaggaa tattgacgtt aagcgctaac
     8521 atctgatccc gtcctttttg ccaggcgttt ttcgtcaggc tatagctgag cgtccagttg
     8581 atatcttcga atgcagtatt taaaccagcc tggaattgct catcgacatt actcgttccc
     8641 caataagttt gatggctacc actcaaatac agtgttgatg agcgcccgag ttgctgagta
     8701 acggtgagct gtaatttccc gcgtttgtta taagcgagat tgtaatagtc ggtgaatttc
     8761 ggcttaacct gaataactcc atcctgtgtt tcgatgttgt agccattcat tcgactgtat
     8821 gttgtatcag cgaaattaaa atacccactg gtcgaataac ggtaacccac taactgaata
     8881 ttcgtgcctg actcattgag cgatttgtta taaagaaaac gcaccgattg tccgtcatgc
     8941 tgactgtcat cgggaagtgt ggaattagcc tgcgtcatat ccacagacag agcgcccagt
     9001 gcccccatat ttttcccgat accgaaatta aacgcacgat aacgatccgc cagttgcgtt
     9061 ccaccatata ttgtccagcc agccggaagg ccgtggagta atgtactctg gaaaaagcgg
     9121 ggtttttcct gttgcgcatt tccactacgg tattctcctg ccgtaatgga ataacgtgta
     9181 tgcccttcac gctgcaaaag cgggactgac gaatagggta cggtaaaaat ctgcgtactg
     9241 ccgtcagcct ctttaatcgt tacctgcaag tcaccactat tacctgcggc atagatatcg
     9301 ttgatggtaa aaggccccgg tggcaccgta ctattataaa tgtcataccc attttgttta
     9361 atagtgacct gtgcagtacc acgagcaata ccgtggatca ccggggcaaa tcctctttgg
     9421 ctatcgggta acatattgtc atctgaggcc aattgtgcgc cgcgaaagtt aataccatcg
     9481 aaaatatcgc cctgagtata accatcaccc agcgtcagcc gggaacgtaa cggaattatg
     9541 tctcgctcaa gccaggtatt gatatgctgc catttatttt tgctacctga tgaactgtcg
     9601 ctactgttat aactccaggt ggtattgtcg cgtaaacgcc acgcaccaat atttaaccca
     9661 ctctgtaggt ttaaatatgc ataatggctg ttacccccaa ttcgattctg tacactattt
     9721 ccgctgaaat tataattgag caatccggca ttaataccgg gatcccataa ctcaggagga
     9781 atataaccac gcgcgcgatt actcataaat gcctgaggga tcgtcaggtt gagtcgctgc
     9841 tgaccaacat ctaaatgcgc agtagcgtca tggatcattg aggttaatgg cacgcaggca
     9901 tcatccgcca gcagattcat accggagaca gaagccgtat tcagccccat actggcaagt
     9961 tgcgcgcgtg tcaggcaggg aacaatccct tgttcactgt cgcccgtgtt aaatgtgaca
    10021 tcacgcgttg ccatataacc attattcaaa tagatatcga cgcgatacgt ccctggcggt
    10081 aattcttgcc cattttcaaa acgcgataaa tcggccacag cctggggatc atccgctaaa
    10141 aagcgcggat taaaatagag ttcggcagat gacaaaggtg cctgtgcggc aaaagcacag
    10201 gcgacaaaga gccggacaaa aaaaccagcc aaacgatgct tacgaatatg caagcattgt
    10261 gtgtttcgct ggtaaagtct taaattcaga tatgacatta ttcctccggt ttaaaatcac
    10321 ccggcagtca attcttttta ttcaggcgaa aaattccccc tgcgttattc cattacgccc
    10381 gtcattttgg gggtaagtgc gccataatca tttattgttc ggtaagtaat attgcttcct
    10441 gcatcagaag gcaatttaac cgtgctttcg cccattggag gcaccaatgc attttcaaga
    10501 acccgggttc cggcattcaa ctctgttacc gtcaggtaat agggtgtcgg gttaatcagc
    10561 gtcagagaat tcgcgctacg acgaaatctt aatttttctg cggcctgatc gggtggcaac
    10621 gctaatttag ccgggcgata gtacagctta atgcggctga taattgcgag ctgtagcgta
    10681 ttctcagtca attttgattt atccattgac ggaatcgctt taacgttcat ccagaataaa
    10741 ctttcccgat cctgtggcaa ttggttattt gttgcatcaa gaatacgtaa ggtattctct
    10801 ctttttccct tcatcgcaaa cagaggtggc gtcacgataa aacgaccatc ctttacacca
    10861 tcggcatttt ccacccatga ttgaattaaa taggtgctat tttcatcatt atttgtcacg
    10921 gcaagttgca cttgtttttg ccctgccgga taaattacgc gggtcgcacc taaggccact
    10981 cccgcttcag cgcgtccggc aaccatcatt gccatgaaca tcaggatacc tgccagcaag
    11041 cagaatgtta tttcctgcga tttccttaca ttgacgtttt tattactcac tgtcctgttc
    11101 ctgttatcac attatctgct gaacaattac tgataggtta aagagaacca ggcctgggca
    11161 ttggcgatgc cgccagtaac ccgacgcccg gtagcacgat atttggcgat gaaatgtagc
    11221 gaagtagagc ctgagtaaag ccgtttccag tttgctggag gacgattaat cggtacgagg
    11281 tttccttcat catcaaataa cgctacgcca atattggtgg ctatccctgg cccctctccc
    11341 acggaaagca catccggatt tttaccatcc gcgacaccgt gaaacgccac acccacacgt
    11401 tcactcacca ccgtgctaca ttcccgtaaa tgaataacaa aaggcaccgg tgcgctatct
    11461 tcccctaccg catgaaaccg gttactgctg attttcccca tattgaccgt catttgttta
    11521 tcaccagctt caactcggca agtttccgca ataatgacgc cctgaaattg catatttccg
    11581 ccgggcagcg tggtattcca tttatttccg gccagagcaa acattggcag caacgacgct
    11641 aatagaaata atctcttcct tttcatcgtt atttttatcg cacaagggtg ggcatccctg
    11701 cccgtaatga cgtccctgaa cctgggtagg ttattgatac tgaaccttga aggtcgcatc
    11761 cgcattagca gcacccgggg ttgcctcgcc gattgcataa taacgcgcct ggaacggaat
    11821 ggtgttggta ccgttattca aggttgtttg tgcactaaat gtcgcaccgt ccagcgccag
    11881 cgcagcaccc gttctgtcca ggatctgcac accaactttt gttgcgctac ccgcagctga
    11941 actctgcaaa gctaatacgt taggatgagt tccatcaatt gcggtaccaa ggaaggcaat
    12001 agcagctttg tctgcaacag tagtgtcaca atcgttcagt tgaatgttaa aaccgacagc
    12061 agaactggtt gctccttctt gtgccagcga tgctgtacga acctgcccta actgaacagt
    12121 ttgatcaaca gagcctgcat caactgcgca agcggcgtta acaacctccc ctttaaagtg
    12181 aacggtccca ccatttaccg tcgtgggtgt agtttcagcc agagccgctg tagaactcag
    12241 ggacagagcc gacagaacaa caattgccag agttttaatt ttcatactgc tttcctttca
    12301 aaaaactatt tctaaatcga catgggcagt cgttctgtac actttgtttt gtcaacgagt
    12361 ttgactgcca acactgcaca gttttccccc aaaagatgaa acatttgggg ccattttgac
    12421 tcatagagga aagcatcgcg cacaaacttt ttca
//
LOCUS       DAZUCC010000151        11951 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZUCC010000151 DAZUCC010000000
VERSION     DAZUCC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.152"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1359)
                     /gene="uidB"
                     /locus_tag="ACHY09_003581"
     CDS             complement(<1..1359)
                     /gene="uidB"
                     /locus_tag="ACHY09_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFS3886506.1"
                     /translation="MNQQLSWRAIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LIN"
     gene            complement(1356..3167)
                     /gene="uidA"
                     /locus_tag="ACHY09_003582"
     CDS             complement(1356..3167)
                     /gene="uidA"
                     /locus_tag="ACHY09_003582"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS3886507.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQEIRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3554..4144)
                     /gene="uidR"
                     /locus_tag="ACHY09_003583"
     CDS             complement(3554..4144)
                     /gene="uidR"
                     /locus_tag="ACHY09_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFS3886508.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLSREASFAQGLRAMIGGILTAS"
     gene            complement(4365..5132)
                     /gene="hdhA"
                     /locus_tag="ACHY09_003584"
     CDS             complement(4365..5132)
                     /gene="hdhA"
                     /locus_tag="ACHY09_003584"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFS3886509.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5244..6272)
                     /gene="malI"
                     /locus_tag="ACHY09_003585"
     CDS             complement(5244..6272)
                     /gene="malI"
                     /locus_tag="ACHY09_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFS3886510.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECSSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6447..8039
                     /gene="malX"
                     /locus_tag="ACHY09_003586"
     CDS             6447..8039
                     /gene="malX"
                     /locus_tag="ACHY09_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFS3886511.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8049..9221
                     /gene="malY"
                     /locus_tag="ACHY09_003587"
     CDS             8049..9221
                     /gene="malY"
                     /locus_tag="ACHY09_003587"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFS3886512.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFASAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSEKGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKRADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9325..10326
                     /gene="add"
                     /locus_tag="ACHY09_003588"
     CDS             9325..10326
                     /gene="add"
                     /locus_tag="ACHY09_003588"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS3886513.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10362..11402)
                     /gene="ydgJ"
                     /locus_tag="ACHY09_003589"
     CDS             complement(10362..11402)
                     /gene="ydgJ"
                     /locus_tag="ACHY09_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS3886514.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEGWGYDMRD
                     GVLTRVEGEERIEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11645..11770
                     /gene="blr"
                     /locus_tag="ACHY09_003590"
     CDS             11645..11770
                     /gene="blr"
                     /locus_tag="ACHY09_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFS3886515.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
BASE COUNT         2902 a         3137 c         2920 g         2992 t
ORIGIN      
        1 gttgattaat tgctgctgaa cttttttacg attatcaatt tcaacaacga tttctttgaa
       61 ttttttatcc gtgagcggat aaaaccagat aatgacgaat gccagtagca taaatccgca
      121 aggtactaag gcaattgatg tgcggatgcc cataataact tccggcgttt gcgcctgatt
      181 ggcgatatat ccgcttaatc ccaaaataaa ggcaggaatt gaacctccga ttgcctgacc
      241 gcatttacgg gtaaatgaga atagtgaata ggtgagccct tcaattcgca cgccggtcag
      301 gtattcaccg tattctacgg tatcagcttc cagtgcccac atcacggtca tggtaacgcc
      361 ctggccaatt gatgcgatgg ccaacgcaac caacgccacc ggcagtgacc agacggaaac
      421 ccagaagaac agcagataac cgcaggttcc cagcaaagcg ccaatcagga aggtattctt
      481 tttaccgatc ctcgcgacca tccccggcac cagcggtgcc gatgccacag taccgaccag
      541 gttttgcacc agtaccagca cagtgaacag cccggtatca tttaacacat agcgcacgta
      601 gaacaacgac gaggcgctga ccgcgaaggt cgaaatcagc acacacagcg caccgatgca
      661 caacataaac agcgggcgat tccgtttcag ggtttgcaga ctgatcttca atgacggctg
      721 cgccacgata cgtaccacat tctcacgcgt cgatttgaag cagatgaagt aaagcaccat
      781 tccggcaatc gccagcacaa ttgtccagaa atgatatacc gacaccatct cttccgggct
      841 ggagttctta atgctcggtc ctatcagaaa tgccaggcag acaaaagtca atgaagcggc
      901 aatcccgcga gccgcgccca gacgggcgcg ggattgtggt tgttgggtca ttgcggtagc
      961 aagtgaacca taaggaatat tcaccaggct gtagcaaagc ccgaggccca tgtaagtcaa
     1021 atatgcatac actactttgc tgctatggct ccagtcggtc ggtacccaga ataccagcac
     1081 gctgaagatc attaacggcg cagtaccgaa gagtaaaaac gggcggaatt ttccccatcg
     1141 ggtattcaca ctgtccacca ctcgtccggc aaagacatcg gcgaaggcat cgaatacccg
     1201 caccagtaac agcatggtgc ccgccgcagc ggcaccgacg ccagcgacgt cggtgtagta
     1261 actcaacagg aagagcgccc ccattgcgaa ggcgaagtta ttggcgacgt caccgaggct
     1321 gtagccgacg atggcgcgcc aggagagttg ttgattcatt gtttgcctcc ctgctgcggt
     1381 ttttcaccga agttcatgcc ggtccagcgt ttttgcagca gaaaagccgc agacttcggt
     1441 ttgcggtcgc gagtgaagat ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag
     1501 gtcgcaaaat cggcgaaatt ccatacctgc tcaccgacga cagcgctgac gcgatcaaag
     1561 acgcggtgat acatatccag ccatgcacac tgatactctt cactccacat gtcggtgtac
     1621 attgagtgca ggccggctaa cgtatccaca ccgtattcgg tgatgataat cggctgatgc
     1681 agtttctcct gccaggccag aagttctttt tccagaacct tctctgccgt ttccaaatcg
     1741 ccgctttgga cataccaacc gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg
     1801 atggtatcgg tgtgagcgtc gcagaacatt acattgacgc aggtgatcgg acgcgtcgga
     1861 tcgagtttac gcgttgcttc cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg
     1921 gtatccggtt cgttggcaat actccacatc accacgcttg ggtggttttt gtcacgcgca
     1981 atcagctctt taatcgcctg taagtgcgcc tgctgggttt ccccgttgac tgcctcttcg
     2041 ctgtacagtt ctttcggctt gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag
     2101 ccgacagctg cagtttcatc aatcaccacg atgccatgtt catctgccca gtcgagcatc
     2161 tcttcagcgt aagggtaatg cgaggtacgg taggagttgg ccccaatcca gtccattagt
     2221 gcgtgatcgt gcaccatcag cacgttatcg aatcctttgc cgcgcaaatc cgcatcttca
     2281 tgacgaccaa agccagtaaa gtagaacggt ttgtggttaa tcaggaactg ttcgtccttc
     2341 actgccactg accggatgcc gacgcgcagc gggtagatat cacactctgt ctggcttttg
     2401 gctgtgacgc acagttcata gagataacct tcacccggtt gccagaggtg cggattcacc
     2461 acttgcaaag tcccgctggt gccttgtcca gttgcaacca cctgctgatc cgcatcacgc
     2521 agttcaacgc tgacatcacc atttgctacc acctgccagt caacagacgc gtggttacag
     2581 tcttgcgcga catgcgtcac cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag
     2641 agcattacgc tgcgatggat cccggcatag ttaaagaaat catggaagta agactgcttt
     2701 ttcttgccgt tttcatcggt aatcaccatt cccggcggga tagtctgcca gttcagttca
     2761 ttgttcacac aaacggtgat acgtacactt ttcccggcaa taacatacgg cgtgacatcg
     2821 gcttcaaacg gcgtatagcc accctgatgc tccatcactt cctgattatt tacccacact
     2881 ttgccgtaat gagtgaccgc atcgaaacgc agtacgatac gctggcccgc ccaacctttc
     2941 ggtataaaga cttcgcgctg ataccagacg ttgcccacat aattacgaat atctgcatcg
     3001 gcgaactgat cgttaaaact tcccggcaca gcaattgccc ggatttcttg taacacgctt
     3061 tcccaccaac gctgatcaat tccacagttt tcgcgatcca gactgaatgc ccacagaccg
     3121 tcgagttttt tgatttcacg ggttggggtt tctacaggac gtaacataag ggactcctca
     3181 ttaagataaa aatactggtc aacctttaat ctgattagtt aaccaattct gtgttgcaca
     3241 cagttatctg tgagaaaccc atggaagaga aatggaaata aaaagaactg acaccgggat
     3301 caaaaaacca aaaactgcaa tacccacagc gtgatataga tcgcattaat ctttaaaacg
     3361 gcgttgacag aagccataaa ataagcaacc gaacgttcgt tcgagcataa tgggaacgat
     3421 aaactctcat gacatatggc tacagtgaat attttggggg gaattctccg aacccgggaa
     3481 ggataatttt tgcacagaga aattacgata tgaaacgcgg gcacagttat caccagcccg
     3541 cgaaatgaga ggatcaggat gcggttaaga taccgccaat catcgcccgc aatccctgag
     3601 caaacgacgc ttcacgcgac agatcttccg cttcgatatc ggccagtaca ccgtaggtca
     3661 gatccagcag taaacgtgaa gtcattgccg tgttgatgtc tgggcttatc tcgccttttt
     3721 gctgggcatc acgcatccgc tgggcaacaa attccgtgat cgtcatatgc ttatttttaa
     3781 gcatggcggc gacctgtggg ttacgcatcc cttccgccat aatttcgacc accagcgccc
     3841 gttgtccaaa ggcctcatgg gtgagagaga caatagactc gaccatatag tcaacgaaat
     3901 gaatcccttc aatcggttcc cggaaacggg ccagcgccct ctcctggtcc tgtaagataa
     3961 tcgcctgaat caaggcttct ttggagatga aatggtgata gagcgtcccg ggactaatgg
     4021 cgcaagattt acagatggct ttcatcgagg cactgtgaaa tccattttct gaaaaaatct
     4081 ctctggcagc attgaggatc cgggtccgtg tcggttgtgc ttcagtctgc atgttatcca
     4141 tcattaaacc attatagtta gcgtgaataa ctagtttacc gcgttgtcag gtgagcgcct
     4201 atcattgctg ttgaatagtg cgaaggcaca ctctattcgt atacttttca ctcggaaatt
     4261 ggttaaccaa tagccgtaat ttggctgcca gttatttagc tgagacgttg atgagtttat
     4321 cccggtaaac aacacgcacg gttgtttacc ggtccgttag tgtattaatt gagctcctgt
     4381 accccaccac cggagacggt gagaatttgt ccgcttaccc agctggctgc aggcgagcaa
     4441 aggaacagcg ccgcgttagc aatatcttgc ggttggccca gacgtctgat cggcgtgtgc
     4501 tgtaacattt tttgttcaat ttctggtgta ataacggatt tcagggcatc ggttaatatt
     4561 gcccccggcg caatgccatt cacccgaata tttttttcac ccaggtcaaa cgccatattt
     4621 ctgaccagat gactggccgc agctttagat gatgcataag aagtcatgtt tatattttta
     4681 ttttctgccg ccatagaagt gatggtcaga ataacgccac cgccattttt ttccatttct
     4741 ggcgcaacaa gttgtgacag atggaaaaaa gaaaacacat tcagttcata agcacggcga
     4801 aaatctgcca ttggcatatc aaacggttta ggaccaccgc caccggcgtt gttaaccagg
     4861 atatcaactt tacccagctt actgacggca aagtctgcca gtgcagagag ttcctgttcg
     4921 gaagtaatat cacaacggca ggcaaatgcc tgaccaccca gttgttgaat ttcatcaaca
     4981 acatggttag ctgcgtcggc gttaatatca ctgaccacca cagatgcgcc agctgtcgcg
     5041 aatgtaatgg cgatttcttt accaatacct gcaccggcac ctgtgatgat ggcacatttt
     5101 ccgtcgagtc tcaggttgtc agaattaaac acataaacct cctgtgaact tcagtaaaaa
     5161 taaaaggcac cgcctgtgtg ccacattttt tatcattaac cttgaggaaa ggacactacc
     5221 aggtatagca gataaagaga ggattatttc gccgcaatga gccgggcggg aataataaga
     5281 ttgcgtgaat gcgtctcttc atgggtgatt ttttgcatca tgcgatccgc aagtgtggta
     5341 ccaagttccc gcgcaggcgt gctggcccag gtaacaggta tatcatcaag tgtggttggc
     5401 gttgcatcgg taaatgccgc caacgaaacc tgttgttcaa agtaacgatc gactccgctt
     5461 tccccgcttt gccgccctgc tttcagcaaa ccaaaccatg cccccatcgc aatagtttcg
     5521 ttatagcaaa ccacagcact gatggtcggg ttatgacgta aaagcgccgt gatagcttcc
     5581 gcggcttgct tctggctgga agagcactcc aacacccaat cgctgtgaaa cggcaggcca
     5641 aattttagta gagttgcaca atagcccccc acccgttctg cccgggttaa tgaggaactt
     5701 tgccctccca gccaggcgat acgctgatgc ccattgcgaa tgagatgctc cgtcaacaac
     5761 tgtgcagcct gcatgttatc cgggcgaacc gtatcaacat catcaagata actggcacgg
     5821 gaagcgaaaa tcaccgggat agctttttct tctgccatcc gtcgcaggtc atcgctgctt
     5881 cctgcagccc cggcaatgac cacaccatcg accccctgat tcagtaacag tgaaaaccgc
     5941 tgtgccagct gctcgccgtc tttaccgccg tgaagcaaaa aaaccatccg tccctgcgct
     6001 tccagagctt ccgtcaatcc ggccgttaat tcggcgtaaa acggcgcaga taaatcacgg
     6061 acgatcaaac caatgacgcc gctttgcccg ccgcgcagcg ccgacgcctg acgattgcgc
     6121 acaaatccca gctcttcaat ggcggcgtta acgcgttctc ctgtggcggt agagattcgc
     6181 cctttgccac ttagcaccag cgaaacggtg cttaccgaca cgcccgcagc cagcgcaaca
     6241 tcatgaatgg ttattttttt ggcggtagcc atgcagatga cctactccct gattatgtga
     6301 cagataaaac gttttacctt ttatttatct tatacccgct attatcgttg cgtaatgtga
     6361 tttatgcctc actaaaattt gataaaacgt tttatcttct cgcgcaattt actgaatcca
     6421 gattgttctc tacgaggagt cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt
     6481 gggagttctt ccagcagtta ggcaaaacct tcatgctacc tgtggcatta ttgtcgttct
     6541 gcggcattat gctcggcatt ggtagttctc ttagcagcca tgatgtcatc accctgatcc
     6601 cggtcctggg caaccccgtg ttgcaggcta tctttacctg gatgagtaag attggctcgt
     6661 ttgcttttag tttcctgcca gtcatgttct gtatcgccat cccgctgggc ctggcacgcg
     6721 aaaacaaagg tgtagcggca tttgctggct ttgtcggtta tgcggtaatg aatctcgcgg
     6781 taaacttctg gttgaccaat aaaggcattc tgccaaccac ggacgccgcg gttctgaaag
     6841 ccaataacat tcagagcatt cttgggatcc agtcgatcga tacagggatc ctcggtgcgg
     6901 tgatcgccgg tattatcgtc tggatgctcc atgagcgttt ccataatatc cgcctgccgg
     6961 atgcgctggc attcttcggc ggtacgcgct tcgtgccaat catctcctcg ctggtgatgg
     7021 gccttgtcgg tctggtgatt ccattagtct ggccaatttt cgccatgggt attagcggct
     7081 taggccatat gatcaacagc gcgggtgatt tcggaccgat gctgtttggt accggtgaac
     7141 gtctgctgtt gccgtttggt ctgcatcaca ttctggtggc attaattcgc tttaccgacg
     7201 caggcggcac gcaggaagtc tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg
     7261 cgcaattgag ttgcccgacc actcacggtt tttctgaaag cgccacgcgt ttcctttcgc
     7321 aaggtaaaat gcctgcgttt ctcggcggcc tgccaggtgc agcgttagct atgtatcact
     7381 gcgcgcgccc ggaaaatcgc cataaaatta aaggtctgct tatttctggc ctgatcgcct
     7441 gtgtcgttgg cggcacgacc gaaccgctgg aattcctgtt cctgttcgta gcgccagttc
     7501 tgtatgtcat ccacgcgctg ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg
     7561 tcaccatcgg taataccgac ggcaatatca tcgacttcgt ggtgttcggt attttgcatg
     7621 gtctgtcaac caagtggtac atggtgccag tggtggcggc aatctggttt gtcgtttact
     7681 acgtcatctt ccgtttcgct atcacccgct tcaatctgaa aaccccgggg cgcgatagcg
     7741 aagttgccag ctcaatcgaa aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg
     7801 ttccggcaat cctcgaagcc ttaggcggtg ccgacaatat tgtcagcctc gataactgca
     7861 ttacccgtct gcgtttgtct gtgaaagata tgtcgcttgt taatgtgcag gcactgaagg
     7921 acaatcgggc aattggcgta gtacaactta atcaacataa cctgcaggtt gttatcgggc
     7981 cacaagttca gtcagtaaaa gatgaaatgg ccggtctgat gcatactgtc caggcataag
     8041 gataagatat gttcgatttt tcaaaggtcg tggatcgtca tggcacatgg tgtacgcagt
     8101 gggattatgt cgctgaccgt ttcggcactg ctgacctgtt accgttcacg atttcagaca
     8161 tggattttgc ctctgccccc tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg
     8221 tgtttggcta cagccgctgg aaaaacgatg agtttctcgc ggctattgcc cactggtttt
     8281 ccacccagca ttacaccgca attgatacgc agtcggtggt gtatggccct tctgttatct
     8341 acatggtctc ggaactgatt cgtcagtggt ctgaaaaagg tgaaggcgtg gtgatccaca
     8401 cacccgccta tgacgcattt tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg
     8461 tcgctttaga gaagcgggct gacggctggt tttgcgatat gggcaagctg gaagccgtgt
     8521 tggcgaaacc agaatgtaaa attatgctcc tgtgtagccc acagaatcct accgggaaag
     8581 tgtggacgtg cgatgaactg gagatcatgg ctgacctgtg cgagcgtcat ggtgtgcggg
     8641 ttatttccga tgaaatccat atggatatgg tttggggcga gcagccgcat attccctgga
     8701 gtaatgtggc gcgcggagac tgggcattgc taacgtcggg atcgaaaagt ttcaatattc
     8761 ccgccctgac cggtgcttac gggattatag aaaatagcag cagccgtgat gcctatttat
     8821 cggcactgaa aggacgtgat gggctttctt ccccttcggt actggcgtta actgctcata
     8881 tcgccgccta tcagcaaggc gcaccgtggc tggatgcctt acgcgtctat ctgaaagata
     8941 acctgacgta tatcgcagat aaaatgaacg ccgcgttccc tgaactcaac tggcagatcc
     9001 cgcaatccac ttatctggcc tggcttgatc tacgtccatt gaatattgac gacaacgcgt
     9061 tgcaaaaagc gcttatcgag caagagaagg tcgcgatcat gccggggtat acctacggtg
     9121 aagaaggtcg tggttttgtc cgtctcaatg ccggctgccc gcgttcgaaa ctggaaaaag
     9181 gtgttgctgg attaattaac gccatccgcg ctgttcgtta accccaattg cgcaacgtaa
     9241 aaaatcgttg cgcaatcgtg gatttttacc ctgctttgtt tttataatgg tgcgcacttt
     9301 tatatccaga aaaagagtgc gaccatgatt gataccaccc tgccattaac tgatatccat
     9361 cgccaccttg atggcaacat tcgtccccag accattcttg aacttggccg ccagtataat
     9421 atctcgcttc ctgcacaatc cctggaaaca ctgattcccc acgttcaggt cattgccaac
     9481 gaacccgatc tggtgagctt tctgaccaaa cttgactggg gcgttaaagt tctcgcctct
     9541 cttgatgctt gccgccgcgt ggcatttgaa aacattgaag atgcagcccg taatggcctg
     9601 cactatgtcg agctgcgttt ttcaccgggc tacatggcaa tggcacatca gctgcctgta
     9661 gcgggtgttg tcgaagcggt gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg
     9721 caggcgaagc ttatcggcat tatgagccgg accttcggcg aagccgcctg tcagcaagag
     9781 ctggaggcct ttttagccca ccgtgaccag attaccgcac ttgatttagc cggtgatgaa
     9841 cttggtttcc cgggaagtct gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg
     9901 catattaccg tccatgcagg cgaagctgcc gggccggaaa gcatctggca ggcgattcgt
     9961 gaactggggg cggaacgtat tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg
    10021 gattttctcg ccgagcagca aattggtatt gaatcctgtc tgacctccaa tattcagacc
    10081 agcaccgtgg cagagctggc ggcacatccg ctgaaaacgt tccttgagca tggcattcgt
    10141 gccagcatta acactgacga tcccggcgta cagggagtgg atatcattca cgaatatacc
    10201 gttgccgcgc cggctgctgg gttatcccgc gagcaaatcc gccaggcgca gattaatggt
    10261 ctggaaatgg ctttcctcag cgcagaggaa aaacgcgcac tgcgagaaaa agtcgccgcg
    10321 aagtaacaaa aatggatggt gctaattgca ccatcctctt ttcacgcaag gcacagcgtc
    10381 gcgcgatgtt tggcggattc gatgcccagc tcaatcaact ccattacctg gattgcctga
    10441 cttgccggaa ccggattttc accatcgcca tttaacgcat cacgaatagc cgcatagtaa
    10501 gccggataat tccctggtac tgtcaacagc gtttcttcga tacgttcctc accttccacg
    10561 cgggtaagta cgccatcacg catatcgtag ccccagcctt cctgcggcag acgctcgcca
    10621 tttttcagtc gttcttcctg tggatcgagg ccatatttca cataactgcc tcgggatcca
    10681 tgcacgatat aacgtgctga ctcagcagct gccagcatgg taccgtgtaa aatgactcgc
    10741 cgctgcggat aggacaagat ggcgtggaaa taatcggtcg actgcgctcc gggccgtaac
    10801 tgtgccaaat ctaccgtcat gctgaccggt aaaccaaata gcgtaatggc ctgatcaaga
    10861 aggtgcggtg ctaaatcgta ccagataccg ctgcctgggc cgccctgttc acgccaacga
    10921 tcgcgaacct gcggacggaa gcggtcaaaa tgagactcaa agtaagcaac ttcacccagc
    10981 acgccttccg cgagcaaacc ttttagcgtc aggaaatcgc tatcccagcg acggttatgg
    11041 aatacagaca gcacacgccc taggcttttt gccagcgctt ccagctctcg cgcttgtgac
    11101 agtgtcacgg taaagggttt atcaacgacc acatgtttac ccgcctcaag cgccgctttg
    11161 gctaacggga aatgggtatc gttgggtgta ggaatgacaa tcaggtctat gttgggatcg
    11221 ttaaacagat gcttcggctc agagacaacc gtaaccgttg gccagtcggc ttttactttt
    11281 gtttcatcgc tgctggagat taccgccagt tccagcccgg gcgtgcccgc aatcaggggc
    11341 gcatggaagg ttttgctcgc ataaccatac ccaatcaacc caacacggat gttgtcgctc
    11401 atgtcattgc ctctcattga agtatgatgg ctatttgaca ctatccttta ccctcgctca
    11461 acagtttaat aacctgccag caataaggga tgttgtttaa cttaagtcaa aaaaatagcg
    11521 aattttccaa cgacaaaagc taaatatcgc aaaaacctca gtaaaaatct tgctggagct
    11581 attattgcta agtaacattt accccctgaa gttaatggat caatcaacag agatgtgggc
    11641 tgtaatgaat cgtcttattg aattaacagg ttggatcgtt cttgtcgttt cagtcattct
    11701 tcttggcgtg gcgagtcaca ttgacaacta tcagccacct gaacagagta ctacggtaca
    11761 acacaagtaa gctctgcgct tgtagagcga catgctgccc gtccgggtgc atgttttcac
    11821 ttgtcggata ttaaaccagg aatttattat cttgttcgat gttgttggtg attgtcagag
    11881 atagtaaagc gcgttactct tttcagagca gtttttcatc cctgaatatt tgccatcagc
    11941 gcattcttat t
//
LOCUS       DAZUCC010000152        11895 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZUCC010000152 DAZUCC010000000
VERSION     DAZUCC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.153"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..957
                     /locus_tag="ACHY09_003591"
     CDS             88..957
                     /locus_tag="ACHY09_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFS3886516.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMVENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1007..1360)
                     /locus_tag="ACHY09_003592"
     CDS             complement(1007..1360)
                     /locus_tag="ACHY09_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFS3886517.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1498..3144)
                     /gene="groL"
                     /locus_tag="ACHY09_003593"
     CDS             complement(1498..3144)
                     /gene="groL"
                     /locus_tag="ACHY09_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS3886518.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3188..3481)
                     /locus_tag="ACHY09_003594"
     CDS             complement(3188..3481)
                     /locus_tag="ACHY09_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS3886519.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3757..5013
                     /gene="yjeH"
                     /locus_tag="ACHY09_003595"
     CDS             3757..5013
                     /gene="yjeH"
                     /locus_tag="ACHY09_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFS3886520.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLSRLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5029..5505)
                     /locus_tag="ACHY09_003596"
     CDS             complement(5029..5505)
                     /locus_tag="ACHY09_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFS3886521.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5842..7278
                     /gene="aspA"
                     /locus_tag="ACHY09_003597"
     CDS             5842..7278
                     /gene="aspA"
                     /locus_tag="ACHY09_003597"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFS3886522.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7397..8698
                     /gene="dcuA"
                     /locus_tag="ACHY09_003598"
     CDS             7397..8698
                     /gene="dcuA"
                     /locus_tag="ACHY09_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFS3886523.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8814..9152
                     /gene="cutA"
                     /locus_tag="ACHY09_003599"
     CDS             8814..9152
                     /gene="cutA"
                     /locus_tag="ACHY09_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFS3886524.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9128..10825
                     /gene="dsbD"
                     /locus_tag="ACHY09_003600"
     CDS             9128..10825
                     /gene="dsbD"
                     /locus_tag="ACHY09_003600"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFS3886525.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIANVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10862..11437
                     /locus_tag="ACHY09_003601"
     CDS             10862..11437
                     /locus_tag="ACHY09_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3886526.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDAWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11544..11619
                     /locus_tag="ACHY09_003602"
     tRNA            11544..11619
                     /locus_tag="ACHY09_003602"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11577..11579,aa:Phe,seq:gaa)
BASE COUNT         2841 a         3081 c         2929 g         3044 t
ORIGIN      
        1 tttaacaact attactacca agcccattta tctggcttat aaattaaaat taattattta
       61 attgagaaat ggttagggag aacctacatg gccataagca tcaagggtgt taataccggt
      121 gttattcgca agagcaataa tttcattgca ctggcgctga aaattaaaga gcctcgcaat
      181 aaagaatcgt tgttctttct gtctgtcatg gaactgcgcg accttctgat tgcgctggaa
      241 agccgtctgc atcaaaagca taaactggat gctgctgcac gccttcagta tgagcaagct
      301 cgcgataaag tcattaagaa aatggtagaa aatatcccag aaattctggt tgatgagctt
      361 aaaaatgccg atattaatcg tcgggttaac acgctggaac ttaccgataa tcagggcgaa
      421 aatcttacct tcgtattaac actacatgac ggtagcaaat gtgagttagt catcaatgaa
      481 ttacagattg aaatgctggc gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt
      541 gaactggcat tacgtattac ttcactgtta gatttcctgc cgttatacga cgttgactgc
      601 caggacaatg gcaatctgga atatgacacc tattcccaac cggaatggaa acataacttg
      661 ttcaaccatt atctggcggt gctttaccgc tttaaagatg aaagtggcaa agaacaattt
      721 agcggcgcag tggtgaaaac gcgtgaagca acgccgggta aagaagtgga ggcgatcact
      781 cgccgaatgc ttgatttcag cccccgactg aaaaaactcg cgggtgtgcc atgtcaggtc
      841 tatgttcgca ccgtagcggc aaataacgcc caaccgctaa cccaggatca atgcttacgc
      901 gcgctgcatc acttacgtgt tcaatccacc agcaaaactg caccacaggc gaaataatct
      961 catctgtttt actacgaggc tggaagtatc cagcctctgg atcacatcag ttcgggcact
     1021 tataaacctg accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg
     1081 acgggctgct gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat
     1141 cgtttgctgc gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc
     1201 tttgcttacc tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa
     1261 tgcgtacact ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta
     1321 gcgcggcacc gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg
     1381 agctggacgc cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga
     1441 aaaaccccca gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta
     1501 catcatgccg cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc
     1561 atcgtttttc ggcaggtcgg taaccatgca ttcggtggtg atcatcaggc cagccacaga
     1621 agctgcgtac tgcagagcag aacgagttac tttggttgga tccaggatac ccatgtcgat
     1681 catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac
     1741 ggtgttagca acaacagacg gttcttcgcc gcagttcaat acgatctgac gcagcggagc
     1801 ttccattgca cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag
     1861 gtcagccagt ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc
     1921 ttctaccgca gcacgggtcg cgtgcagggc atcttcaacg cgtgcttttt tctctttcat
     1981 ttcaacttcg gtagcagcac ccactttgat aactgcaacg ccgcctgcca gtttcgctac
     2041 gcgctcctgc agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat
     2101 ctgagcaaca cggccctgga ttgcagcttc ttcacccacg ccatcaatga tggtggtggt
     2161 gtctttgttg atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag
     2221 ctccataccg atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag
     2281 catagcttta cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc
     2341 acgcatggtg ttaacaacca gagttgccag cgcttcgcct tctacatctt cagcgatgat
     2401 cagcagcggt ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt
     2461 ggagattttc ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc
     2521 cggcttgttg ataaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac
     2581 gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac
     2641 tttgtccatc gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt
     2701 accaacctga gcaatcgctt tagagtcaga gcatggtacg gacagcgctt tcagttcttc
     2761 aactgcagcg gtaaccgctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc
     2821 agcaacagct ttcagacctt cagtgatgat agcctgagcc agtacggttg cagtggtggt
     2881 accgtcgcct gcagcgtcgt tcgctttgga ggcaacttct ttcaccatct gcgcacccat
     2941 gttttcgaac ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat
     3001 ggtcggtgca ccgaaagatt tatccagaac tacgttacgg cctttcggac cgagggtaac
     3061 tttcactgca tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc
     3121 gaattttacg tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt
     3181 gcgcggatta cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt
     3241 cattgtcgat cttctcggat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa
     3301 ctttcacgtc cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca
     3361 gcacttcgcc gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag
     3421 atttagtttc aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca
     3481 ttgataactc tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg
     3541 tttcccggct ggtgaccaga gaaatgggga tgaggcttcg ccccttcaag ggggaaaaag
     3601 aaaaaaaatt ctgatcagca caaaatcggg tgaaaaccct gattcacctc acatttcatc
     3661 gcaatttctt catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca
     3721 gttgcccaaa atttgggcgc aatcggacat caacccatga gtggactcaa acaagaactg
     3781 gggctggccc agggcattgg cctgctatcg acgtcattat taggcactgg cgtgtttgcc
     3841 gttcctgcgt tagctgcact ggtagcgggc aataacagcc tgtgggcgtg gcccgttttg
     3901 attatcttag tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc
     3961 gcaggcggcg tcgcgcactt cgtcggtatg gcgtttggtt cgcggcttga gcgagtcacc
     4021 ggctggctgt ttttatcggt cattcccgtg ggtttgcctg ccgcactaca aattgccgcc
     4081 gggttcggcc aggcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt
     4141 acgctggcgt tggtgtggta tatcggtact cgcggtgcca gttccagtgc aaatctacaa
     4201 accgttattg ccggacttat cgtcgcgctg attgtcgcta tctggtgggc gggcgatatc
     4261 aaacctgcga atatcccctt cccggcacct ggtaatatcg aacttaccgg gttatttgct
     4321 gcgttatcag tgatgttctg gtgttttgtc ggtctggagg catttgccca tctcgcctcg
     4381 gaatttaaaa atccagagcg tgattttcct cgtgctttga tgattggtct gctgctggca
     4441 ggattagtct actggggatg tacggtagtc gtcttacact tcgacgccta tggtgaaaaa
     4501 atggcggcgg cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt aggagcgtta
     4561 tggattgcct gcgtgattgg ctatctggcc tgctttgcca gtctcaacat ttatatacag
     4621 agcttcgccc gcctggtctg gtcgcaggcg caacataatc ctgaccacta cctgtcacgc
     4681 ctctcttctc gccatatccc gaataatgcc ctcaatgcgg tgctcggctg ctgcgtggtg
     4741 agcactttgg tgattcatgc tttagagatc aatctggacg ctcttattat ttatgccaat
     4801 ggcatcttta ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaagga
     4861 cgttatcgac tactggcggt ggttggcggg ctgttatgcg ttctgttact gacaatggtc
     4921 ggctggaaaa gtctctatgc gctgatcatg ctggcggggt tatggctgtt tctgccaaaa
     4981 cgaaaaacgc cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg
     5041 atcgtcttta tgatcaaggc ggtcgcggtc atcatccttt cgctggtact caccatcaaa
     5101 agtattaccg ccaccggtcc cggcgctaaa accgccgcct ggcatgcgag aaaagcgcaa
     5161 atgcggcatc aacttcactg tcagatgctt ttgcaccggc ggcaacaaaa gcagaagacc
     5221 gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga tcagcgaaac
     5281 acttttaatc atctccgccg ctgggttttc acccgccgcc attttttgct gcatcagcac
     5341 gaaattctta aagccctggt tacgtaccag tgacataccg ataactgacg tgaatataac
     5401 cagcacgaga gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc
     5461 aatatagaca taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa
     5521 tagcagccgg ttaaacccgg ctacctgaat gggttgcgaa tcgcgttcag cttatattgt
     5581 ggtcattagc aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg
     5641 tgatctattt cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat
     5701 tacggtagaa atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat
     5761 cgtcggtcga aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc
     5821 agcttgaaaa agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt
     5881 accagggaag ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac
     5941 ttctatatca gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg
     6001 gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg
     6061 aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc
     6121 ccggtagacg tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg
     6181 ctggccaata tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac
     6241 ccgaacgacc atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt
     6301 atcgcagttt actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc
     6361 tttgaacgta aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag
     6421 gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa
     6481 gaagtgaaaa acatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca
     6541 gcaatcggta ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg
     6601 gctgaagtca ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac
     6661 tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa
     6721 atctgtaacg acctgcgctt gctctcttca ggcccacgtg ccggcctgaa cgagatcaac
     6781 ctgccggaac tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg
     6841 gaagtggtta accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca
     6901 gcagaagcag gtcagctgca gttgaacgtt atggagccgg tcattggcca ggccatgttc
     6961 gaatccgttc acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc
     7021 atcactgcta acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact
     7081 tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa
     7141 accggtaaga gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt
     7201 gacgatattt tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact
     7261 gatgaaagcg aacagtaatc gtacagggta gtacaaataa aaaaggcacg tcagatgacg
     7321 tgcctttttt cttgtgagca gtaacttaaa aataacaatc taatatcaac ttgttaaaaa
     7381 aacaaggaag gctaatatgc tagttgtaga actcatcata gttttgctgg cgatcttctt
     7441 gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg tgctggttct
     7501 tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca ttatcatggc
     7561 ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc tggttcatca
     7621 gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac cgatcgtgac
     7681 ctatttcctg acaatctttg ctggtactgg caacatctct ctggcgacac tgccagttat
     7741 cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctgtcta ctgcagtggt
     7801 atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc
     7861 cgtaatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca tcccgtccac
     7921 cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact ccaaactctc
     7981 tgacgatccg atttatcgca agcgtctgga agaaggcctg gttgaactgc gcggtgaaaa
     8041 gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt
     8101 tggcgttgtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg aaaaaccgct
     8161 gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc tgaccaccgt
     8221 tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag caggtatgag
     8281 cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt ccaacaacat
     8341 cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc tgctggccgt
     8401 catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa aagcactgat
     8461 gccgatggct ctggcactga acgtttctcc gctgaccgct gttgcttctt tcgctgcggt
     8521 gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac agatggatga
     8581 cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca tcccgggtac
     8641 tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg
     8701 atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc cttcccccgt
     8761 ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg
     8821 atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg gatgaagcga
     8881 cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga
     8941 tccccggcgc tacttctctc tattactggg aaggtaagct ggagcaagaa tacgaagtgc
     9001 agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc ctgaagtctc
     9061 atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga gacacagatt
     9121 acctctcatg gctcaacgca tctttacgct gatcctgcta ctttgcagca cttccgtttt
     9181 tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc aagcctttgc
     9241 ttttgatttt cagcaaaacc aacatgacct gaatctgacc tggcagatca aagacggtta
     9301 ttacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg ccaacgtgca
     9361 gctgccacaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga tttaccgcga
     9421 tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac
     9481 ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt
     9541 aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgttccgc agcaagagca
     9601 gcccaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc
     9661 ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggtatcgtgc tgggtggtaa
     9721 acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc aggggatggc
     9781 actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt tccaggcggc
     9841 gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc tggcgatgtc
     9901 aatgtttggt ttgtttaccc tgcaactccc ctcttcgctg caaacgcgcc tcacgctgat
     9961 gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatgggag cgattgccgg
    10021 actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgttgt atatcgccca
    10081 aagcgggaac atgtggctgg ggggcggcac gctttatctc tatgcgttgg gcatgggcct
    10141 gccgctgatg ctaataaccg tctttggtaa ccgcctgctg ccgaaaagcg gcccgtggat
    10201 ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct tcctgctgga
    10261 gcgagtgatt ggcgatatat ggggattacg cttgtggtcg gcgcttggtg tcgcattctt
    10321 tggctgggcc tttatcacca gcctacaggc caaacgcggc tggatgcgcg tggtgcaaat
    10381 aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg catttggtgc
    10441 aacacatacc gcgcaaactc agacgcatct caactttaca caaatcaaaa cggtagatga
    10501 gttaaatcag gcgctcgttg aagccaaagg caaaccagtg atgttagatc tctatgccga
    10561 ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc aggtgcaaaa
    10621 agcgttagca gacacggtct tacttcaggc taacgtaact gccaacgacg cacaagatgt
    10681 ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt ttgacggaca
    10741 aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc
    10801 acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag
    10861 cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac aagggattgc
    10921 caacaccacg cttgagatgg ttgctgaacg tgtggattac ccactggacg agctacgccg
    10981 cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta gccaacagat
    11041 agatgcctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac aaaagctgct
    11101 ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg gctgtctatt
    11161 tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac tggccgatca
    11221 gcaaaaaagc gcggcctacg atttcaccca cgaactgtta accacactgg aagttgacga
    11281 tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa gccgaatgct
    11341 ggtgaatcgt agccaggcgg atgtcgacac cgcacatcgg ctggcggaag atatcctgcg
    11401 cttcgcccgc tgccgccagg gtggtgcttt gacctgacag aaacacagaa aagaagcgat
    11461 ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg agatgtgcag
    11521 attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag gggattgaaa
    11581 atccccgtgt cggtggttcg attccgcctc cgggcaccat ataccagaac acgctggtta
    11641 agggatgaga tccttgacgg gcggttaata caggatgcgg catcaaaggg tctccgtttg
    11701 tgtaagttgt ggttacactg attgagttcg gtatcgtatc ctcaaacagg gtgtcttttg
    11761 cagagacgcc ctgttctcgt taacactgta tgcttacatt gctgattcag cctgtcaaga
    11821 tttattcccc cctttgcaaa tcttgctcac tgtgcgctca tctgtataca aactcagggc
    11881 atcttccagt gtctg
//
LOCUS       DAZUCC010000153        11776 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZUCC010000153 DAZUCC010000000
VERSION     DAZUCC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.154"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..341)
                     /gene="yehE"
                     /locus_tag="ACHY09_003603"
     CDS             complement(60..341)
                     /gene="yehE"
                     /locus_tag="ACHY09_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFS3886527.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(604..1713)
                     /gene="apbC"
                     /locus_tag="ACHY09_003604"
     CDS             complement(604..1713)
                     /gene="apbC"
                     /locus_tag="ACHY09_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFS3886528.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPNMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1845..3878
                     /gene="metG"
                     /locus_tag="ACHY09_003605"
     CDS             1845..3878
                     /gene="metG"
                     /locus_tag="ACHY09_003605"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS3886529.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEEIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4020..7808
                     /locus_tag="ACHY09_003606"
     CDS             4020..7808
                     /locus_tag="ACHY09_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFS3886530.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAGLKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEISQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVTALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISHPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPSEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSWLLKYPEHAITGLLPAALGKASEAQDNARAALRMLTENGH
                     QPLLQEIARRYNQPEVTDAVNALLALDALDNHPTKIPTLPTFYQPSLWTRPLLKANAQ
                     SLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDASGSRLKDLPKPNKSDDELQANNAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTAD
                     DDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWAGRKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAV
                     GMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE
                     "
     gene            7818..11450
                     /gene="yehI"
                     /locus_tag="ACHY09_003607"
     CDS             7818..11450
                     /gene="yehI"
                     /locus_tag="ACHY09_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFS3886531.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQHEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPWLSTQARAVLNSYLSAPPKTVIDSTDNSQMPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYPGRVEEIMNGWSSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRPEKSVWNLYLLAQLPREMALPFWLRINEKKHLSAGEDYFLSIFGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPA
                     RMPKAPDFWHPLMWSRPRLIINHQLITDDALEIIGEMLRFTQGGRFHSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSTR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRTDDDQLKAPE
                     ALARLKGLKKDTTQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTEGMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYGLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11512..>11776
                     /gene="yehK"
                     /locus_tag="ACHY09_003608"
     CDS             11512..>11776
                     /gene="yehK"
                     /locus_tag="ACHY09_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFS3886532.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETV"
BASE COUNT         3062 a         3013 c         2886 g         2815 t
ORIGIN      
        1 taaatcattc atgtgaatga tttaagcttt ctaaatccga ttaaaaccag ggtggcaaat
       61 taatcgtagc gatttaacac gattcgacgt ttgctatcgc tatctgcagc aatcacttcc
      121 gtcgttacac ctttaactgg cgaagaaacc tgatgacggg tatcaacatt atacgttaac
      181 tgaccacaat gctgttgagg ttgaccatta accatagcca tactgcatgt cgggggactg
      241 atcctaccat taatcgttaa tgtggcagtt tctgaagaga acgcgggaga ggccaatcca
      301 taggcaagaa aaataattcc tgacagccaa tacttattca ttgaacgtta tccctgtagt
      361 aaaggttatg cctggataga atgagtgcat aacaaactat agctgtacat ccactacaca
      421 gccacgaagg atgataatga agcattgcct gtacgatcaa tcgactttgt agagtttcgg
      481 acgaaggtcc gcagaatatt cgcagcatta aataagtgtt cattaatgcg gcaaaaacaa
      541 atacaccgca tcaatgtaac atctctgcgg tgtatgaaga ataaccagaa tggttaatac
      601 gcgttagacc gcgcggaagg aaatctcgcc tggaatgact tcaccctgcc agtaaagctg
      661 agctgcaacg cggtcagcca gctggcggta gatagcggta aattcgctct caggacggct
      721 gataaccgtt ggcgtgcctt tatcgagatc ctcacgtaag gagatgtgta acggcatctg
      781 ccccagcagc tgtgtgtggt atttctcggc cagtttctct gcaccaccgg taccgaaaat
      841 cggttcatgg tgaccacagt tactgcaaat atgcacactc atgttctcga caataccgag
      901 caccggaacc tcgactttct cgaacatcac aatgcctttt ttcgcatcga tcaacgcgat
      961 gtcctgcggg gtggtgacca cgactgcacc ggttactgga atgttttgtg ccaatgtcaa
     1021 ttgaatgtca ccggtacctg gcggcatatc aagcaccaga tagtcgagat ccggccacaa
     1081 ggtttcctgc aacatttgca ttaacgcctt gctcgccatt gggccacgcc acaccatcgc
     1141 attgtcgtct gtcaccagat aaccaattga attagttgcc aggccatgag acataatcgg
     1201 tgccatatgg gtaccgtccg gcgaagttgg acgctgattt tctgcaccca gcatgttagg
     1261 gattgatggg ccgtagatat cggcgtccag aatgccgact ttcgcccctt ccgccgccag
     1321 tgccaacgcc aggtttaccg ccgtggagga tttacccacg ccacctttac cggaactgac
     1381 cgcgataatg ttcttcacgc cgttaattcc cggctgattc ttcacgcgtt tcagagtggc
     1441 tatgttatgc gacagtttcc agtcgatagc tttcgctccg gtgatgcgca gcagttcggc
     1501 gctacattgc tcttttaact cttcgaacgc gctatgccag acgaatggca taaccagttc
     1561 cacatgcagc gtgtcgtcca tccaggcgac atggtgcaac gctttcagcg ttgtgaggtt
     1621 atgcttcagg gttgggtgct gaaaattggc cagtgtcccg gcgaccattg cgcgcaaggc
     1681 ttctggtgat ttggcctggg attgttcgtt catcccgact ccttttttgt atagataaac
     1741 catcagttga tagtttacct gaagaatata gagaagtact tacttaacat tttcccattt
     1801 ggtactatct aagccctttt cactattaag aagtaatgcc tactatgact caagtcgcga
     1861 agaaaattct ggtgacgtgc gcactgccgt acgctaacgg ctcaatccac ctcggccata
     1921 tgctggagca catccaggct gatgtctggg tccgttacca gcgaatgcgc ggccacgagg
     1981 tcaacttcat ctgcgccgac gatgcccacg gtacaccaat catgttgaaa gctcagcagc
     2041 ttggtatcac tccggagcag atgattggcg aaatgagtca ggagcatcag actgatttcg
     2101 caggctttaa catcagctat gacaactatc actcgacgca cagcgaagag aaccgccagt
     2161 tgtcagaact tatctactct cgcctgaaag aaaacggttt tattaaaaac cgcaccatct
     2221 ctcagctgta cgatccggaa aaaggcatgt tcctgccgga ccgttttgta aaaggcacct
     2281 gcccgaaatg taaatccccg gatcaatacg gcgataactg cgaagtctgc ggcgcgacct
     2341 acagcccgac tgaactgatc gagccgaaat cggtggtttc tggcgctacc ccggtaatgc
     2401 gtgattctga acacttcttc tttgatctgc cctctttcag cgaaatgctg caggcatgga
     2461 cccgcagcgg tgcgttgcag gagcaggtgg caaataaaat gcaggagtgg tttgaatctg
     2521 gcctgcaaca gtgggatatc tcccgcgacg ccccttattt cggttttgaa attccgaacg
     2581 cgccgggcaa atatttctac gtctggctgg acgcaccgat tggctacatg ggttctttca
     2641 agaatctgtg tgacaagcgc ggcgacagcg taagcttcga tgaatactgg aagaaagact
     2701 ccactgccga gctgtaccac ttcatcggta aagatattgt ttacttccac agcctgttct
     2761 ggcctgccat gttggaaggt agcaacttcc gcaagccgac caacctgttt gttcacggct
     2821 atgtaacggt gaacggcgcg aagatgtcca agtctcgcgg cacctttatt aaagccagca
     2881 cctggctgaa tcattttgac gctgacagcc tgcgttacta ctacactgcg aaactctctt
     2941 cgcgcattga tgatatcgat ctcaacctcg aggatttcgt tcagcgtgtg aatgctgata
     3001 tcgtcaacaa agtggttaat ctggcgtccc gtaacgcggg ctttatcaac aaacgttttg
     3061 acggcgtgct ggcaagcgaa ctggctgacc cgcaactgta caaaaccttc actgatgccg
     3121 ctgaagagat tggcgaagcg tgggaaagcc gtgaatttgg taaagcagtg cgcgaaatca
     3181 tggcactggc tgacctggct aaccgctatg tcgatgaaca ggctccgtgg gtggtggcga
     3241 agcaggaagg tcgcgatgct gacctgcagg cgatttgctc tatgggcatt aacctgttcc
     3301 gcgtactgat gacttacctg aagccggtac tgccgaaact gactgaacga gcagaagcat
     3361 tcctgaatac ggaactgacc tgggatggta tccagcaacc gctgctgggc cataaagtga
     3421 atccgttcaa agcactgtat aaccgtatcg acatgaagca ggttgaagca ctggtggaag
     3481 cgtctaaaga agaagtgaaa gccactaccg cgccagttac tggcccactg gcagatgatc
     3541 cgattcagga aaccatcacc tttgacgact tcgctaaagt tgacctgcgc gtggcgctga
     3601 ttgaaaacgc agagtttgtt gaaggttctg acaaactgct gcgcctgacg ctggatctcg
     3661 gcggtgaaaa acgcaatgtc ttctccggca ttcgttctgc ttacccggat ccgcaggcac
     3721 ttattggtcg tcacaccatt atggtggcta acctggcacc acgtaaaatg cgcttcggta
     3781 tctccgaagg catggtgatg gctgctggtc ctggcgggaa agatatcttc ctgctaagcc
     3841 cggatgccgg tgctaaaccg ggtcatcagg tgaaataatc cccccttcaa ggcgctgcat
     3901 cgacagcgcc ttttctttat aaattcctaa atttgttttc ttgcgatttt gtctctctct
     3961 aacccgcata aatactggta gcatctgcat tcaactggat aaaattacag ggatgcagaa
     4021 tgagacactt tatctatcag gacgaaaaat cacataaatt ctgggcggtg gagcagcagg
     4081 gaaacgaact gcacatcaac tggggtaaag tcggcaccaa cggacaaagc caggtaaaaa
     4141 gttttgcgga tgcagcagcg gcggaaaaag cgggactgaa gctgattgca gagaaggtga
     4201 agaaggggta tgtggagcaa gcgaaggata attctttgca accttcccaa acggtaacgg
     4261 actctctcaa ggtagctgac ttatccacca ttattcagga acaaccctct ttcgtagcag
     4321 aaacccgtgc ggctgacaaa aatacagatg ctgttttacc gtggctggcg aaagatattg
     4381 ctgtcgtttt tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag
     4441 ttcctgttca gcaagcagac aaattgaccc aattacgtcg cttagcctgt agtgtgtcgc
     4501 aacgggataa taaaacagcc acatttgact tcagcgcctg ttctttagaa tggcaaaaca
     4561 ccgtcgccca ggcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcacccg
     4621 taatggctgt actcactgca cttgaaatga aatgcacaag atataaagag cgtgaggatg
     4681 ttatggatca gattattcag gaaggcggtc tggaatatgc tactgatgta ataatacatc
     4741 ttcaacaaat atctattgaa tgggattatg tcaacaataa tattgttttc ctgtcgtctg
     4801 gcatttcacc tgattacttg cagcaatatt ccagctttga attacgccta cgtaaacatt
     4861 tatcactggc ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc
     4921 cacatattcc agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa
     4981 ttgcacatga aatttcccag cgtctactgg ggcaaaagaa attaccctcg cttgagtggc
     5041 taaaaatagt ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat
     5101 atgccatttt tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc
     5161 aaggtgttac agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg
     5221 tgttgcgtca tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata
     5281 ctaaacgctg tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg
     5341 cagcactggc ggaacttcta gtgcaaaaag aagagaatag ttggcgcatt atgctaatga
     5401 cgatgcttat ctcgcatccg acactagcag aacaggtcat tccctggctt tcgacacccg
     5461 cagttgcagt gctgaaatca tgtcagcaac aactgacaca gccctcaaac catgccagcg
     5521 ccgatctact gccggccata gtagtctccc caccatggct ttcgaaaaag aaaaaatcgc
     5581 cgattccggt gctggattta gcaccgctca acctcgaatc catatgcaca ataaccgaca
     5641 cagaagctaa agagtttcaa actcattggg actgggaacc gcataagcca agtgaaggtg
     5701 ctaaaaattt cctttactcg ctggggtatc gccgctggga ttttgacaca tataaatata
     5761 tcggcgcttc tgactcggca attgacgcat gggaacgtga ggacttcgct acactcatcc
     5821 aaatgtttaa agcccaccat gcaccgtatc agggggagtg gcatctcaac tcacttccct
     5881 ttttacctat gcaaaaagcg attaagctat gggaatttct cagcaaagag ccgcataccg
     5941 cgataaaacc agtcatgctt tatttgcgac tggcaggtat gagtggtttt ctacactcgt
     6001 tttcacgcta ccctcaagaa ggttttgcgg ttgctaatta ttttgccgca acggaactgg
     6061 ctcctgccgt cgcccgcgcc ttcaacaaac tcaaaacatt acgacaagac gccagtagct
     6121 ggctgctgaa atacccggaa cacgccatta ccggcctgct gcctgcagcg ctcggcaaag
     6181 ccagtgaagc acaggacaac gcccgtgctg ccttgcgtat gcttaccgaa aacggtcatc
     6241 agccattgct gcaagaaatc gcccggcgtt ataaccagcc ggaagtaacc gatgcggtga
     6301 acgctctact tgcgctcgat gccttagata atcacccgac aaaaatcccc actcttccga
     6361 ccttttatca gccatcgctc tggacgcgcc cgttattaaa agcaaacgcc caatcactgc
     6421 ctgatagcgc actcctccac ctcggtgaaa tgctccgctt ccctcaggaa gaggcactgt
     6481 atccgggatt attgcaggtg aaagatgcct gtaccaccga ctcactggct gaatttgcct
     6541 gggatctgtt taccgcctgg cagaccgctg gcgcgccgtc gaaagagagt tgggcgttca
     6601 ctgcgttagg cgttctcggt aacgatgaca ccgcccgcaa actgacgcct ttgattcgcg
     6661 cctggcctgg tgaatcccag cataaacgcg ccaccgtcgg gttggatatt ctcgctgcta
     6721 tcggtagtga tatcgccctg atgcagctta acggcatcgc ccagaaactg aaattcaaag
     6781 cattacagga gcgggcaaaa gaaaaaattg ccgacattgc cgagagccgc gaactcacgg
     6841 tggcggagct tgaagatcgg ttagcaccgg atctcggtct ggatgataat ggttcgttgt
     6901 tgctggattt cggcccacgc cagttcaccg tcagctttga tgaaacctta aaaccgtttg
     6961 tgcgtgacgc ttccggcagt cgtctgaaag acctgcccaa accaaacaaa agcgatgatg
     7021 aattacaggc gaacaatgct gttaaccgct acaaattgct gaaaaaagat gcgcgtaccg
     7081 tcgccgccca gcaggtagca aggctggaat ccgccatgtg cctgcgtcgc cgctggtcac
     7141 cggaaaactt tcagctcttc ctggttgagc atccgctggt ccgccactta acccgccgtc
     7201 tgatttgggg cgtttatagc gccgaaaacc agctactggc ttgctttcgc gtagcggaag
     7261 ataacagcta cagcaccgct gacgatgatc ttttcaccct gccggaaggc gatatctcta
     7321 ttggtattcc tcacgttctg gaaatatcac cgacggatgc tgccgccttc gggcagcttt
     7381 ttgccgacta cgaactgtta ccaccgttcc gccagcttga tcgtaacagt tacgcgctga
     7441 cagaagccga gcgcaatgcc agcgaactga cacgctgggc aggcagaaaa tgcccgagtg
     7501 gtcgggtaat ggggctggca aataaaggct ggatgcgtgg tgaaccgcag gacggtggct
     7561 ggatcggctg gatgatcaaa cctctgggcc gctggtcgtt aatcatggaa atcgatgaag
     7621 gttttgcggt cggcatgtcg ccagccgaac tcagtgccga gcaactctta agcaagctgt
     7681 ggctatggga aggcaaagcg gaaagctatg gctgggggag taattcaaca caggaagcgc
     7741 agttctccgt actcgatgcc atcaccgcca gcgagctaat taacgatatt gaagccctgt
     7801 ttgaataagg aaagagcatg gacaaggaat taccgtggct ggcggataac gcccaactgg
     7861 aactgaaata taaaaaaggc aaaacaccac tcagccatcg caattggcca ggcgaaccgg
     7921 tacctgttat cactgaaaac atcattcaga cactgggtga tgaattgcta caaaaagcag
     7981 agaagaaaaa aaacattgtc tggcgttatg aaaacttttc actggagtgg cagtccgcca
     8041 tcacgcaggc catcaacttg atcggcgaac acaaaccctc aatcacggcc cggacaatgg
     8101 cggcgctagc ctgtatcgcg caaaatgata gccaacagtt gctcgacgaa atcgtccaac
     8161 acgaggggct ggaatatgca actgaggtgg tgattgcacg ccagtttatt gtacggtgtt
     8221 atgagagtga tcctctggta gtaacattgc aatatcagaa cgaggactat ggctatggtt
     8281 atcgctcaga aacctataac gaattcgatc tccgattgcg taagcatctc tctctggcag
     8341 aggaaagctg ctggcagcgt tgcgccgaca aactcattgc cgcactgcca ggaataacca
     8401 aagttcgccg cccttttatt gcgctgaacc tcccggaaaa acccgaaatc gccaacgagc
     8461 tggtaagcct tgaatgttcg caaactcatt ttcgctcaaa agagtggtta aaagttgttg
     8521 ctgatgaccc taaggcggtg aaagaactcg cacgttactg gagccaggat atatttagcg
     8581 atcgagaagc cagctacatg tcgcatgaaa accacttcgg ctacgcggcc tgcgctgccc
     8641 ttttgcgcga acaaggactg gcagccgttc cgcgcctcgc gatgtatgca cataaagaag
     8701 attgcggcag tctgctggta caaattaacc atccgcaagt catccgcacc ttgctactgg
     8761 tggctgataa aaacaaaccc agcctgcaac gtgtggctaa atacagtaaa aacttccccc
     8821 atgcgacgct cgccgcactg gcagaactgc tggcgttaaa agccccccct gcgcgccctg
     8881 gttatcccat catcgaagac caaaagctgc ctgcacagca aaaagcacgt gatgaatact
     8941 ggcatacgct gttacaaacg ctgatggcat cgcagccaca actggcagaa gaggtgatgc
     9001 cgtggttaag tactcaagcc cgggcagtgc tgaatagtta tttatcggca ccgcccaaaa
     9061 cggttattga tagcaccgat aacagccaaa tgcctgaaat cctcgtttca ccaccgtggc
     9121 gtagtaagaa aaaaatgaca gctccacgtc ttgatttggc accgctcgaa ttaaccccgc
     9181 aagtttactg gcaaccaggc gaacaagaga ggcttgcctc cactgagtct gcccgttatt
     9241 tcagcacgga atctcttgct gaacgcatgg aacaaaaaag tggacgagtt gtattacagg
     9301 aactgggttt tggggatgat gtatggctgt ttctgaatta tatactcccc ggaaaactgg
     9361 atgctgcacg caattcactc attgttcagt ggcattacta cccagggcgg gttgaagaga
     9421 tcatgaatgg ctggagctcc ccggaagctc aattagcaga acaggcgctt cgcagcggtc
     9481 acgtagaagt gttaattaac atatgggaaa atgacagcta ctcacgttat cgtccggaaa
     9541 agagtgtctg gaacctgtat ttattggcac agttgccgcg tgagatggct ttgcctttct
     9601 ggctacgtat caatgagaaa aagcatctgt ctgcgggtga ggactatttt ctcagtatct
     9661 tcggattgga tgctctacca ggtctgctgt tggctttttc acatcgtcca aaagaaacat
     9721 ttccgttaat tttaaatttt ggcgcaacag aactggccct gcccgttgcc cgcgtctggc
     9781 gacgtttcgc ggcgcagcgt gatctggccc gccagtggat tttacaatgg ccggaacata
     9841 cggctactgc acttatccct cttgtcttca ccaaacccag cgataatagc gaagctgcat
     9901 tacttgccct acgtttactt tacgaacagg gacatggtga attgctgcaa accgcggcaa
     9961 accgctggca gcgtacagat gtatggcctg ccctggagca tttacttaaa cagagtccaa
    10021 tagagattta cccggcacgc atgccaaaag cccctgattt ctggcatccg ctaatgtggt
    10081 ccaggccgcg cctaatcatt aatcatcaac ttattaccga tgacgctctg gaaattatcg
    10141 gcgaaatgct gcgctttacc caggggggac gttttcatag cgggctggaa caactgaaaa
    10201 cgttctgcca gccgcaaacg ctggcagctt ttgcctggga tctcttcact gcgtggcaac
    10261 aagctggtgc ccccgcaaaa gacaactggg catttctggc gttaagtctc tttggtgacg
    10321 aaagcacggc acgggatcta acgacacaga tcctcgcctg gccacaagaa ggcaaatcta
    10381 cccgtgctgt cagtggcctg aacatcctta ccctgatgaa taatgatatg gcgctgatac
    10441 agctgcatca tatatcgcaa cgggcgaaat cccgcccctt acgtgataac gcggcggaat
    10501 ttcttcaggt ggtcgcagaa aatcgcgggc taagccagga agagttagcg gacagattag
    10561 tcccaacact gggccttgat gatccgcagg cgttgagttt tgattttggt ccccggcagt
    10621 ttaccgttcg cttcgatgaa aaccttaacc cggttatctt tgatcagcaa aacgttcgcc
    10681 agaaaagcgt tccccggttg cgcaccgatg acgatcaact gaaagcgccc gaggcactgg
    10741 cccgattaaa agggctaaaa aaagatacga ctcaggtgag caaaaacctg ctcccccgtc
    10801 ttgaagctgc cctgcgtacc acccgacgct ggtcgctggc agattttcat tctctgtttg
    10861 ttaatcatcc ctttacccgt ctggttaccc agcgattaat atggggcgtg tatccggcaa
    10921 atgaaccgcg tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg
    10981 cgcaagatga gcccattgac ctgcctgcgg atgctctgat tggcattgcc cacccgttag
    11041 aaatgacaga aggaatgcgc agtgaatttg cacagctttt tgccgattac gaaattatgc
    11101 cgccttttcg ccagttggcg cggcgcacgg tgctgctcac acctgacgag tcaaccagta
    11161 acagcctgac tcgctgggaa ggtaaatccg ctaccgttgg gcagcttatg ggaatgcgat
    11221 acaaaggctg ggagtcaggc tatgaggacg catttgtcta tggcctgggc gagtaccggc
    11281 tggtccttaa gttttcaccc ggttttaacc actacaatgt tgatagcaaa gcgttaatga
    11341 gcttccgttc tcttcgagtg taccgtgaca ataaatccgt cacttttgcc gaacttgatg
    11401 tgtttgattt gagtgaggcg ttaagcgcac ctgacgtcat tttccattaa aaacacagcg
    11461 ggcagtgatg caactgcccg ttatcaacac gacttaccag cggggaaagc gatgatcgtg
    11521 caaaaagagc tggttgctat ttacgattat gaggtccctg tacctgaaga tccgttttcc
    11581 ttcagacttg agatccataa atgctctgaa ttatttacag gttccgtcta tcgactggag
    11641 cgattccggc tacgtccaac atttcatcaa cgtgatcgag aagatgctga cccgctaata
    11701 aatgatgcgt tgatttatat aagagatgag tgtattgatg agcggaaatt acgaggtgaa
    11761 tcacctgaaa ctgtaa
//
LOCUS       DAZUCC010000154        11721 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZUCC010000154 DAZUCC010000000
VERSION     DAZUCC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.155"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..275
                     /locus_tag="ACHY09_003609"
     CDS             <1..275
                     /locus_tag="ACHY09_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein acyltransferase"
                     /protein_id="HFS3886533.1"
                     /translation="YICYEALFPEKYVHNGVVITQSDYIRLNNGRGYKTTLVNGSLLA
                     KFSVAPNTKLINVQNYGGTIVFNNDWEIDWDIYNKSKKEHFFVVTL"
     gene            complement(423..1358)
                     /locus_tag="ACHY09_003610"
                     /pseudo
     CDS             complement(423..1358)
                     /locus_tag="ACHY09_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1702..2322)
                     /gene="entD"
                     /locus_tag="ACHY09_003611"
     CDS             complement(1702..2322)
                     /gene="entD"
                     /locus_tag="ACHY09_003611"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFS3886534.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFEPASFHEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAVHALREFGFKYAPPVGERRQPLWPAGLYGSISHCQATALAVVSHQ
                     PTGIDIEEIFSAQAAEELKDSIMTPAEYELLVVSNLPFSVVLTLAFSAKESAFKASKL
                     QTDQGFLDYQIVNWNKQRIIICWENEIFAVQWQIRGKQLITLCQHD"
     gene            complement(2476..3824)
                     /locus_tag="ACHY09_003612"
     CDS             complement(join(2476..3344,3344..3824))
                     /locus_tag="ACHY09_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS3886535.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKLFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNMVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKK
                     PPGAGEKTKVIRSLKCGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIF
                     HEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAE
                     NILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVK
                     RMLNKGLESLAEGEKPLLHNDQGWHYRIKSYQSALADKGLVQSMSRKGNCLDNAVMEN
                     FLGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPLEYRTEYQKAG"
     gene            complement(3934..6194)
                     /gene="fepA"
                     /locus_tag="ACHY09_003613"
                     /pseudo
     CDS             complement(3934..6194)
                     /gene="fepA"
                     /locus_tag="ACHY09_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
     gene            6437..7639
                     /gene="fes"
                     /locus_tag="ACHY09_003614"
     CDS             6437..7639
                     /gene="fes"
                     /locus_tag="ACHY09_003614"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFS3886536.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFRWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMQANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            7642..7860
                     /gene="ybdZ"
                     /locus_tag="ACHY09_003615"
     CDS             7642..7860
                     /gene="ybdZ"
                     /locus_tag="ACHY09_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFS3886537.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQVSCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            7857..>11721
                     /gene="entF"
                     /locus_tag="ACHY09_003616"
     CDS             7857..>11721
                     /gene="entF"
                     /locus_tag="ACHY09_003616"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFS3886538.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPETIDLRTNIDPHGTAQSLMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFVRQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DSQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERALQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIR"
BASE COUNT         2612 a         3072 c         3065 g         2972 t
ORIGIN      
        1 tatatatatg ctatgaagca ctgtttcctg aaaaatatgt acacaacggg gtcgtaataa
       61 cacaatctga ttacataaga cttaataacg gtaggggata taagactaca ttggttaacg
      121 gtagcttact tgcaaagttt tccgttgcac caaacaccaa gttaataaat gtccagaatt
      181 atggtggaac tattgttttt aataatgatt gggaaattga ctgggatatt tataacaaat
      241 ccaaaaaaga acattttttt gtggttactt tataatacag cgagggagtg ttgttgaaat
      301 ttttatgagc tatttacgcg cccacatatt attcaagtct tgcatatata tgctgagtag
      361 ttaaattccc ctgtgctatg ccagttactc cattcgcttc taagtagctg atcctaatac
      421 cgctacactt tagccagccc atgctttcca ccggggaatg ggctgacggt atccataaag
      481 tgacgaacct ttttcaacag ttgccacatt gaacggcact ggtgatttcg ggttattgtt
      541 tcatgaagcg cctgccacaa ccgttcgaca tgattgaccc atggcgagta aattggctga
      601 taaatcaccc tgaactttgg gtttgctttc agccagcgca acgtttcaca gcttttgtgg
      661 gtgatgtagt tatcgacaat gagcgtaatc ttttttgccc gtcagtatgt ctccttcaga
      721 tgttttaaca gcctgataaa taaggatgaa cctttactgt ttccacccac gtagctgact
      781 ttgcccgtac cactgtgcag cgcaccggca agatagtact tttcattccg gcccggcgta
      841 acaacgcgct tttgctgccc gcgtaactgc cagtctgcgc cgattttggg attgaggtgg
      901 atctccactt catcttcgta aaacacaggg tgttctatgc tacaactgac taacgctaca
      961 ttaatcgctg ccattttttc ttccttattc gggccccgga tacgcagtgt tggtgctgct
     1021 ctgcgccata ccagttcggc tgcaggtaac catcgccggg agaatgctta acaagctcac
     1081 gcagcaatgc gcaaatatgc tcgaacggcc agcgcctctc acgtcctgac ggcaatgact
     1141 tcaggccttc aacacccgac agcgtgaacc agttaatcca gcgaccaatc gatgagcgag
     1201 cacagcagag tgttctggca gcatgactga cggtgtcacc acgatgcagc atcagcatgg
     1261 ctgtgagccg ccgggcgtgg ttttttgtcc tttgttttat ggatcgtttt ctggatcagg
     1321 cgtcgttcag ttctgggtat tggtgctatg atcggcactg ctccgtccgg ttggtgattt
     1381 gggatattca gcgattgatc agatcgcata atcgactctg taacaacctg aacggtgtcg
     1441 gggtggtaac ataggttagg aaacagggtg cggatgccat catggcgcgg gtcatcagtc
     1501 cactaacgac gccggatata tggcagcaaa tcagtggttg tatattgaac aactataata
     1561 gcgtcagatc tcaccattat aacggtgggc tgacaccgaa aaaattagag aacagataac
     1621 gttcttagcg taaaacggtg gtcaatatta cttgactact acaagcaatg gttgctggct
     1681 aaccacagta agggttatcc attaatcgtg ttgacaaagc gttatgagtt gttttcctct
     1741 tatctgccat tgcacagcaa atatctcgtt ctcccaacaa ataatgatcc gctgtttatt
     1801 ccagttcact atctgataat ccagaaaacc ttgatctgtc tgtaacttgc ttgccttaaa
     1861 cgcactctct ttcgctgaaa acgccagtgt aagcaccaca gaaaagggta aattgctgac
     1921 cacaagcagc tcgtattctg ctggtgtcat aatgctgtct ttcaactctt ccgctgcttg
     1981 tgcagagaag atctcttcga tatcaatacc cgttggttga tgggatacca ccgctaatgc
     2041 cgtagcctgg cagtgactaa tactgccgta taacccggca ggccaaagtg gctggcgacg
     2101 ttcaccgaca gggggggcgt acttaaagcc aaattcccgt aaagcatgaa cagcagcaat
     2161 ccgtccggct aaatgttcgg ctttgcgttt ccgtccggca tgttgcagtt gcgcataatg
     2221 cggcagccag agtaaatcct gttcgtgaaa acttgctggc tcgaactcaa caaaatgcag
     2281 cgtatgtccg gcaaagggga gggaggtatg cgtagttttc atatcgacca tattagagac
     2341 tgatgacaaa cgcaaaactg cctgatgcgc tacgcttatc aggcctacac tatcctgcaa
     2401 tatattgaat tggcaagatt tttgtaggcc ggataaggcg ttctgaactg aaccccaaaa
     2461 cctggacagt ttcagttaac cggctttttg atactcagtc cggtattcta acgggctgag
     2521 tcccccaaga ctgagtttta ttcttttttg attccagtaa gttatgtatt cgttaacggc
     2581 attttccagc tcttctacac ttctgtagtc ccggcgatag tacatttctt ctttcaggtg
     2641 acctaagaaa ttctccatta ccgcattatc aagacagttg cccttgcgtg acatgctctg
     2701 cactaaccct ttgtctgcca gggcagactg ataacttttt atccggtaat gccacccttg
     2761 atcattgtgg agcagcggtt tttctccttc tgcaaggctt tccagcccct tatttaacat
     2821 gcgcttcacc agctcctctg taggcctgca ggcagtttcc cacgccacga tttccccgtt
     2881 aaacaaatca agtatcgggg acaggtacag tttttgtccg ccagccctga attcggtgat
     2941 atctgtcacc catttctcac agggagcttc cgctttgaac tgccgctgaa ggatattttc
     3001 tgcagcgagt cccatatttc cccggtaaga ccggtatttc ttcaggcgca caggagactt
     3061 caggccgagc tgttgcatca gttttcgcac cgttttgcca ctgaacttaa gacctctttt
     3121 ctgaagctca caatggatcc gccggtaccc ataacatccc cggtgttcat ggaagatgga
     3181 ggctatgagc tgtttcacat cagcatactt gtccttagcc ttctgcagtg acagctggta
     3241 gtacagcgtg cttctggcca gccctgcagc ctttaacagg tcagactggc agtgtccgca
     3301 cttcaggctc cggatgactt tcgttttttc tccagctcct ggcgcttttt ttccaggaga
     3361 tgctcctgca tggctttcag atatgcattt tctgcgcgaa gataacgcag ctcattcttc
     3421 atctcttccg gcgtcatgtt tgcctcgttt tcatgggtat catcagaacg ggtcatgctt
     3481 ttctccttcc tgcgcctgat aaaggcctca ttaccagatt tgcggtagcc ttttatccag
     3541 tttttaacca tgttgtggct gggaatgtta aaccgggcag ctacccgggg tagagactca
     3601 gaatgcccga gagcataaag aaccacgcaa agtttatctt caggagaata gctacgcttg
     3661 tgtctgcaat ccagtgcccg aggaccgtga agaagaaaaa gattaatcca gtgtgagagg
     3721 ctggtgtgag aaagttgaaa gagttttgcc gtagaaatga tgcctgcatg accagccagg
     3781 taatgattta cagcggcaag ttttacttca aatgaatgtt tcataaactg caccttcaga
     3841 tgtcagatta tgtccaacat ttggggtgca gttcagtttc acgccgcatc cggcatgaat
     3901 tacgcgcact ttgtcaacaa tctgacgtta gcatcagaag tgagtattaa tgctcatata
     3961 ccacgtacgt cccggctcgt tataggtata cgccccggca ccggcgatat agttggctcc
     4021 tgccagatcg cccgtggtct gggcattacc cgcacgccac aaacgtttgt cgaacaggtt
     4081 gtccacgccg ccggtcagac tgacattctt cgtcacatcc caggtcgcgc tcagacccac
     4141 aatgctgtaa ggactgattt ctttggtttc cggtccaacc gctggctgac ctttatagtt
     4201 gtacttcttc ggctgctgct tgccgtacca ggtgaaggtt gtttgcatcg acaaatcttc
     4261 ccgtgcctgc cagctcagcg ttgagttcaa cgtatactcc gggatgatcg acaaacggtc
     4321 gcccgtggtt ttgttttcac tcttcagcat ataagtgatg ttattggtcc acatcaccgt
     4381 ttcgctaacc ggtacgttta acgatccttc cagaccttca accaccgctt tcggtacgtt
     4441 atcccactga tagagatcgg tgccgactgc gttttgccct acagccacat agcccgcttc
     4501 aatcttattg cgataatcgt tacggaacca ggtgacgccc gccagccaac cgtcgcgttt
     4561 gaactccaga ccaatctctt tgttgatgct ggtttccgct ttcaggtcat cattaccttg
     4621 cagatagcag ccgcccgcgc tggcatagca gccctgacct ttactgtaga gaatgtagtt
     4681 tgggttagtc tggtacagac tcggcgcttt ataagcgcga gcaatgccca ttttcagcgt
     4741 gaagtcatcg cctaaacctt gcgatatgtt cagcgccgga ctccagttat tgccgacaat
     4801 actgtgatga tcgaaacgca gccccggcgt tacgatggtg ctgtcagtta gctccatatt
     4861 gttttcggca aacagcgaga aaatttctgc ttttgaatac gggctacggt cggtggcact
     4921 cacgccatca atagcgccgc cggtattcgt cccggtgagc gcttgggtgt tggaactcaa
     4981 gtccttcatc cgctgctgat tccactccgt acccagcgtc agcgtctggt taacgaggaa
     5041 atcaatcggc aggttaactt cgctgtgcag catcacgtca tcgagatcga tatcgacgaa
     5101 atcctgtgtc gctttttcgt taaatttccc ttcggtaccg cccgccagac cttccggaat
     5161 acgcgagtta cgggtgtgtt cgtactgcac ccagttgctg gtggtcacgc cgttatccca
     5221 gccgccgttc catgtcagcg agtagttctg gcgatacaga cggttggttt catcgccata
     5281 tttcgagcgg gtataggagt cagagttggt gttctgggta tcgcccgcat acaggttacc
     5341 ctggcggctg taacccgctt ccagttccag cgattgcaat ggtgcgaagt cccagcgcac
     5401 cacgccgtta atatctttgt tgatcacccc ttatctttgt tgatcacccc ttcgcgcccg
     5461 gctggtaacg tcgtggcata cgttccggca cgcgcggact gatggccctg gttgatatcc
     5521 cacgcgtcag cctgagtttt gtcgaggttg ccatacaagc ggaagctgaa ttcgtcgccc
     5581 agcggaccgg tgaggctaaa gttagtgcgt ttggtggcac cttcctcttt atgttctggc
     5641 gcattgaaat aagcgtccca cgagccgtgc cactcaccac tgcctttttt ggtaatgatg
     5701 ttaaccacgc cgcccgccgc gccgttgcca taacgcgcag ctgccggacc acgcagaact
     5761 tcaatacgtt caatcatttc aggtggcacc caggaggtat caccacgggt atcgcgctcg
     5821 ccacgccagc cctgacgcac cgagttacgg ctgcttaccg gcttgccgtc aatcaaaatc
     5881 agcgtgtttt ccggacccat accgcgaata tcaatctggc ggttattccc acgctgacca
     5941 ctggtggagt taccggtcag gttaacgcct ggcatggtac ggatgatctc cgacacatcg
     6001 cgggcaaccg ggtttttgcg gatttcatct gcggtgatgg tcgaaacgcc aggtgcctgc
     6061 aagttctgct cggcggcggt aacgacaatg gtatcgtcat gtgaaacagg agtatcggtc
     6121 ggctcttgtg cctgcgctac cccataaatc cccagattga ccaacaaggc cagggaatga
     6181 atcttcttgt tcattgtttt attcctgcat ttttgccacg aattgcaact gtcgggcatg
     6241 gtcgtcatca acacgacgca tcccgctacc gcgaaaacct ttgatcctga aagacacgca
     6301 gtgcagttgg ttaattaatg tccgcgcttc ccacggcgcg ccattacgct attgcaaatg
     6361 caaatagtta tcaataatat tatcaatata tttctgcaat caatgaaaaa ttgcacagta
     6421 aacatggggt tatggtgtga cggcgttaaa agtaggaagt gagagctggt ggcagtcgaa
     6481 acatggcccg gaatggcagc gtctgaatga cgaaatgttt gaggtcactt tccggtggcg
     6541 tgatccccag ggttctgaag aatactcgac gataaagcgc gtatgggtct acatcactgg
     6601 tgtgaccgat caccatcaga acagccagcc ccagtcgatg cagcgaattg caggcaccaa
     6661 cgtctggcag tggacgacac aacttaatgc caactggcgc ggcagctact gttttattcc
     6721 caccgaacgc gatgacattt tttctgcacc atcccccgat cgcctcgaat tacgtgaagg
     6781 ctggcgaaaa ctattacccc aggcgatagc cgatccgctg aatccacaaa gctggaaagg
     6841 cgggcgaggg cacgctgttt ctgcactcga aatgccgcaa gcgcccctac aaccgggatg
     6901 ggattgtccg caagcgccag aaacacctgc caaagaaatt atctggaaaa gtgagcggtt
     6961 gaaaaattca cgccgtgtat ggatttttac cactggcgat gcaacagcag gagaacgtcc
     7021 gctggcagtt ttgctcgatg gcgaattttg ggcgcagagt atgcctgtct ggcctgtgct
     7081 gacttcgttg acccatcgtc agcaacttcc tccagccgtg tatgtgttga tcgacgctat
     7141 tgacaccacg caccgcgccc acgaactgcc gtgtaatgcg gatttctggc tggcagtaca
     7201 gcaagagtta ttacccctgg tgaaagctat tgcccctttt agcgatcgcg ccgatcgcac
     7261 cgtggtcgcc gggcagagtt ttggtgggct ttccgcgctg tatgccggac tgcactggcc
     7321 tgaacgcttt ggctgtgtat taagccagtc aggatcgtac tggtggccgc atcggggcgg
     7381 gcagcaagaa ggtgttttac ttgagcaact taaagctggt caagttagtg ccgaaggtct
     7441 gcgcattgtg ctggaagcgg gtattcgcga gccgatgatc atgcaggcca atcaggcgct
     7501 gtatgcgcaa ttacacccca taaaagaatc cattttctgg cgtcaggttg acggcggaca
     7561 tgatgcgctt tgttggcgcg gtggcttgat gcaggggcta atcgacctct ggcaaccact
     7621 tttccatgac aggagttgaa tatggcattc agtaatccct tcgatgatcc gcagggagcg
     7681 ttttacattt tgcgcaatgc gcaggggcaa ttcagtctgt ggccgcaaca atgcgcctta
     7741 ccggcaggct gggacgttgt gtgtcagccg cagtcacagg tgtcctgcca gcagtggctg
     7801 gaagcccact ggcgtactct gacaccggcg aatttcaccc agttgcagga ggcacaatga
     7861 gccagcattt acctttggtc gccgcacagc ccggcatctg gatggcagaa aaactgtcgg
     7921 atttaccctc cgcctggagc gtggcgcatt acgttgagtt aaccggagag gttgatgcgc
     7981 cattactggc ccgcgcggtg gttgccggat tagcgcaagc agatacgctg cggatgcgtt
     8041 ttacggaaga taacggcgaa gtctggcagt gggtcgatga tgcgctgacg ttcgaactgc
     8101 cagaaactat cgacctgcga accaacattg atccgcacgg tactgcgcag tcattaatgc
     8161 aggcggattt gcaacaagat ctgcgcgtcg atagcggtaa accactggtc tttcatcagt
     8221 tgatacaggt ggcggataac cgctggtact ggtatcagcg ttatcaccat ttgctggtcg
     8281 atggcttcag tttcccggcc attacccgcc agatcgccaa tatttactgc gcatggctgc
     8341 gtggcgaacc aacgcctgct tcgccgttta cgcctttcgc tgatgtagtg gaagagtacc
     8401 agcaataccg cgaaagcgaa gcctggcagc gtgatgcggc attctgggcg gaacagcgtc
     8461 gtcaactgcc accgcccgcg tcactttctc cggcaccttt accggggcgc agcgcttcgg
     8521 cagatatttt gcgcctgaaa ctggaattta ccgacgggga attccgccag ctggctacgc
     8581 aactttcagg tgtgcagcgt accgatttag cccttgcgct gacggctttg tggctggggc
     8641 gattgtgcaa ccgcatggac tacgctgccg gatttatctt tatgcgtcga ctgggctcgg
     8701 cggcgctgac ggctaccgga cccgtgctca acgttttacc gttgggtatt cacattgcgg
     8761 cgcaagaaac gctgccgcaa ctggcaaccc gactggcagc acaactgaaa aaaatgcgcc
     8821 gccatcaacg ctacgacgcc gaacaaattg tccgtgacag tgggcgagcg gcaggagagg
     8881 aaccgctgtt cggtccggta ctcaatatca aggtatttga ttaccaactg gatattcctg
     8941 gtgttcaggc gcaaacccat accctggcaa ccggtccggt taatgacctt gaactggccc
     9001 tgttcccgga tgaacacgga gatttgagta ttgaaatcct cgccaataaa cagcgttacg
     9061 ctgagccaac gttaatccag catgctgaac gcctgaaaat gctgattgcc cagttcgccg
     9121 cggatcctgc gctgttgtgc ggcgatgtcg atattatgct gccaggtgaa tatgcgcagc
     9181 tggcgcagat caacgccact caggttgaga ttccagaaac cacgctgagc gcgctggtgg
     9241 cagaacaagc ggcaaaaaca ccgcatgctc cggcgctggc agatgcgcgt tacctgttca
     9301 gctatcggga aatgcgcgag caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg
     9361 ttaaaccagg cgacagcgtg gcggtggcgc taccgcgctc ggtctttttg actctggcgc
     9421 tacatgcaat tgttgaggct ggtgcggcct ggttaccgct ggataccggc tatccggacg
     9481 atcgcctgaa aatgatgctg gaagatgcgc gtccgtcgct gttaatcacc accgacgatc
     9541 aactgccgcg ctttgccgat gttccagatt taaccagcct ttgctataac gccccgctta
     9601 caccgcaggg cagtgcgccg ctgaaacttt cacaaccgca ccacacggct tatatcatct
     9661 ttacctctgg ctccaccggc aggccgaaag gggtaatggt cgggcagacg gctatcgtta
     9721 accgtctgct ttggatgcaa aaccactacc cgcttacagg cgaagatgtc gttgcccaaa
     9781 aaacgccgtg cagttttgat gtctcggtgt gggagttttt ctggccgttt attgccgggg
     9841 ctaaactggt gatggctgaa ccggaagcgc accgcgaccc gctcgctatg cagcaattct
     9901 ttgccgaata tggtgtaacg accacgcact ttgtgccgtc gatgctggcg gcatttgttg
     9961 catcgctgac tccgcaaacc gctcgccaga attgcgcgac gttgaaacag gttttctgta
    10021 gtggtgaggc cttacccgct gatttatgcc gcgaatggca acagttaacc agcgtgccgt
    10081 tgcataatct atatggcccg acggaagcgg cggtagatgt cagctggtat ccggcttttg
    10141 gcgaggaact ggcacaggtg cgcggcagca gtgtgccgat tggttatccg gtgtggaata
    10201 cgggcctgcg tattcttgat gcgatgatgc atccggtgcc gccgggtgtg gcgggagatc
    10261 tctatctcac cggtattcaa ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca
    10321 gccgctttat tgccgatcct tttgcccctg gcgaacggat gtaccgtacc ggagacgttg
    10381 cccgctggct ggataacggc gcggtggagt acctcgggcg cagtgacgat cagctaaaaa
    10441 ttcgcgggca gcgtatcgaa ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg
    10501 tcgaacaagc cgttacccac gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg
    10561 cgcgtcagtt ggtgggctat ctggtgtcgc aatcgggcct gccgttggat accagcgcat
    10621 tacaggctca gcttcgcgaa acattgccac cacatatggt gcccgtggtt ctgctgcaac
    10681 ttccacagtt acctcttagc gccaacggca agctggatcg caaagcctta ccgttgccgg
    10741 aactgaaagc acaagcgccg gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg
    10801 cggcattctc gtcgttgctg ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc
    10861 ttggcggtca ttcgctactg gcaatgaaac tggcagcgca gttaagtcga cagtttgttc
    10921 gtcaggtgac gccggggcag gtgatggtcg cgtcaactgt cgccaaactg gcaacgatta
    10981 ttgatggtga agaagacagc acccagcgca tgggattcga aaccattctg ccgttgcgtg
    11041 aaggtaatgg cccgacgctg ttttgtttcc atccggcctc cggttttgcc tggcagttta
    11101 gcgtgctctc gcgttatctc gattcacaat ggtcgattat cggcattcag tcgccgcgcc
    11161 ctcatggccc catgcagacg gcggcaaacc tggatgaagt ctgcgaagcg catctggcaa
    11221 cgttacttga acaacaaccg cacggtcctt attacctgct gggttattca cttggcggta
    11281 cgctggcgca gggcattgcg gcgcgactgc gtgcccgtgg cgaacaggtg gcatttcttg
    11341 gcttgctgga tacctggccg ccagaaacgc aaaactggca ggaaaaagaa gccaatggtc
    11401 tggacccgga agtgctggcg gagattaacc gcgagcgcga ggccttcctg gcggcacagc
    11461 agggaagtac ttcaacggag ttgtttacca ccattgaagg caactacgct gatgctgtgc
    11521 gcctgctgac gactgctcat agtgtaccgt ttgacggtaa agcgacgctg tttgttgctg
    11581 aacgcgcgct tcaggaaggt atgaatcccg aacgcgcctg gtcgccgtgg atagccgagc
    11641 tggatatcta tcgtcaggat tgtgcgcatg tggatattat ctctccaggg gcgtttgaaa
    11701 aaattgggcc gattattcgc g
//
LOCUS       DAZUCC010000155        11696 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZUCC010000155 DAZUCC010000000
VERSION     DAZUCC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.156"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(144..1517)
                     /locus_tag="ACHY09_003617"
                     /pseudo
     CDS             complement(144..1517)
                     /locus_tag="ACHY09_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1716..2762)
                     /gene="fbpC"
                     /locus_tag="ACHY09_003618"
     CDS             complement(1716..2762)
                     /gene="fbpC"
                     /locus_tag="ACHY09_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFS3886539.1"
                     /translation="MSQKNFVELRNVTKRFGSNRVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKDALTMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTAGVR
                     PEAITLNNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(2774..4852)
                     /locus_tag="ACHY09_003619"
     CDS             complement(2774..4852)
                     /locus_tag="ACHY09_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3886540.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFREATREQRSRHYFDASWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMNVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILTVWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPLYLAGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(4921..5952)
                     /locus_tag="ACHY09_003620"
     CDS             complement(4921..5952)
                     /locus_tag="ACHY09_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS3886541.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(5949..7265)
                     /gene="uhpC"
                     /locus_tag="ACHY09_003621"
     CDS             complement(5949..7265)
                     /gene="uhpC"
                     /locus_tag="ACHY09_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFS3886542.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLNANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWSGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(7338..8879)
                     /locus_tag="ACHY09_003622"
     CDS             complement(7338..8879)
                     /locus_tag="ACHY09_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFS3886543.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALLILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPAILAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(8879..9508)
                     /locus_tag="ACHY09_003623"
     CDS             complement(8879..9508)
                     /locus_tag="ACHY09_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS3886544.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWPALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            complement(9784..10965)
                     /locus_tag="ACHY09_003624"
     CDS             complement(9784..10965)
                     /locus_tag="ACHY09_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3886545.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     T"
     gene            11281..>11696
                     /locus_tag="ACHY09_003625"
     CDS             11281..>11696
                     /locus_tag="ACHY09_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS3886546.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLE"
BASE COUNT         2859 a         3109 c         3048 g         2680 t
ORIGIN      
        1 tatttatgtc ataacttttc tgaaagatag ctggtaattg ccttgagaaa gtttttatat
       61 gttattctgt aaaacaggat aacagaaaat aattatgtct gaaataagta gatgatgtcc
      121 cctctgagct ggctgtctct tgatcagata tcctgatcaa gagacttcat caccaggtaa
      181 ccctcaacca tatcctgaag tctgaaccag tcatcccaca taactaccca accggggcgg
      241 cctgtgcgtt tgctgtcatg tcatcgcccc agtttagcca gtttcagact ggctcatttc
      301 aggatcggtg cctgcgccgg aagcggctct ccttccagct taacccacag cagtttccac
      361 tctgtcggcg tcagtatttt ctcacaactg tcattttgtg tttcttcact gacacctccc
      421 tgccgcaggc ccggcacccg taccgcgata aacgccttga taaccaccat gcgctcaagg
      481 ttatcccggg tctgaattcg cagcgattcc acacatgtac caccactttt ccacgccttg
      541 tggtattcct ctatcagcca gcgtcgctcg taatggctga cgatacgtcg cgcatcggcg
      601 gcactcgcca ctttttctga cgtcagcaga tgccagcagg cactgtcctc tgcctgctcc
      661 cggcaacaga catacgtgag cgagagcgcc tggccgctgt tgtcgggatt ttttatgctg
      721 acttcgctgt aactgatgaa catccgggcc tggcgggctg cccgcccgcc tttttgcatc
      781 acattcagcg tgtggcttcc cgcggttgcc aggacttccg gcagttcgaa gagcttgccg
      841 ggtgcttctt ccagccggct attctgtgca gcacgcacca cgaagcgctg tccgtggctg
      901 actttataat gcaggtaatg ccagatatcc gcttcccggt cacagacagt gattacccgt
      961 ttctgtatct cccccagccg ttcggccatg cgctccgaag cctgctgcca gcggtaactt
     1021 tctttttctt cacagggacg ttcttttcgc tggtgcttaa caccgtaggt gtccgtggcc
     1081 cgactccagc tctgctgttc gataagagcg actggcaggg cgctgtcggg ggcgtatatc
     1141 aggacagagt gagccagcag cccgcgcgtc ttcgggttag tggtggtatt ccccaggtca
     1201 tcagatgccg tactgtggct gaagttaatg gtggaggtgt cttccagtgc ggggagcagc
     1261 ggatgtgcct cacatgccct tgcagtggcg gtaaatccgg ctccggaaat ggcttccggg
     1321 gacactgacg ggttacgcat caggcggtac gcaccttcaa cctgagcagt ggagtgggat
     1381 tatttcacaa tagaaagacc tgcatgctga gcgagagaag aggccagtga cacaaggcgt
     1441 cgtgtacgac gcggatcacc gagacgggca tgtccagact gctcgttagc ccatgaataa
     1501 caatcagaaa gtaccataac agacccgaag aaaatgaaat ataagagaag atcaacgggt
     1561 gaagaaaaag ttcaaaaaat cgctgccggg gaggaaggaa actaccggat ggaaagagtc
     1621 ccccctaaag cagactgaca gacatcacaa atccccaggt ggtgatttgt gtataagaga
     1681 cagctgagct ggaggggacc gcttatgcgc tatttttatg ccgcatccgc taaaacaaac
     1741 atcccgtaag ggtggatttc gaggtaatag tgctcaccaa tatcgggctg taaacgggtg
     1801 gcgttgactt gcaataaaat ctcctgacca tgccattcta cggtcacttc gtactgcggc
     1861 cccatgtaag cgacatggcg aataacacaa cgctgactct cttcgccgtg attgtttaag
     1921 gtgatggctt ccgggcgaac gccggcggtg ccgctacctt gcgccgggaa atgcgctgcg
     1981 cgcggcaggc gataaccgta gatatcgaca tactcttcgc taaagttcgc cgggaacagg
     2041 ttggcgtcgc ccataaaact tgccataaat cgggaggctg gctgacgata gagatcctgc
     2101 ggtgagccaa tctgcatgat gtgaccttta ttcatcacca gcacagtgtc ggacaccgcg
     2161 aaagcttcac tttgatcgtg agtcacgtag agtgacgtga tattaaactg cttttgcagc
     2221 tcgcggatct tatcgcgcat gctgcggcgc aggttggcat cgaggttact taacggctca
     2281 tcaaacagca gcacctttgg cttgaggatc aacgcacggg ccagagccac acgctgttgc
     2341 tgaccaccgg aaatctggtc gacatagcgg tcttcgaatc cttccagatc caccattgtc
     2401 agcgcatctt tcacccgctg cttcacttca ctacgcgaca cgccaagcat tttcagccca
     2461 tagccaacgt tttcgcccag cgacatatgc gggaacagag cgtatgactg aaacaccatg
     2521 cagatatcgc gttgctgaat ggaacgatgc gtgacatctt cgccatctat aaaaatttgc
     2581 ccttcgctcg gtttttccag cccggcaacc agacgcagaa tggtggtttt gccgcatccg
     2641 gaaggaccga gcagcgtcac catttgccct tgtgggatgg tgagattgat attgtcgata
     2701 accctattac tgccgaatcg tttagtgacg ttgcgcagtt ccacaaaatt tttctgactc
     2761 atagtgcgct ccattacgcc tggtttttgg ctttagaacg ggaaatacgt gcttcaccga
     2821 tcagccagtc aaagaggaaa ataatcgcca gcatcaccac gatcagaatc gaaccgtagg
     2881 cgatggctac gccatattcg ccgtcttcta cgcggttaag gatgtaggcg gttgccacgc
     2941 gtgtgtccgg tgtgacgaga aagacgatgg cgctgactgt agtgatggcc cgcacaaagc
     3001 tgtaaatcag ggccgagaga atggccgggc gtaacagcgg cagtaaaatg tgcgtaatgg
     3061 ttcgcaggga acccgcacgc aggctaagtg aggcttcatc cagcgatttg tcgatctgcc
     3121 ccagacctgc aatcccggcg cgaataccca ccggaacgtt acgcatcacc atcgagataa
     3181 taacaattgc tgccgttccg gcgagataga gtggggcgct attaaaggca agaatatacg
     3241 agacacccgc cactgtaccc ggtacggcaa agcacagcat ggtggtgaac tcgatggtct
     3301 ttttgccttt gaattgttga cgcaccacca cccaggcgat gagcaggcca aagatagcgg
     3361 taatgggggc tgcaatcccg gcgtagagca gggtatccag cagtgaaggc cacgcgccgt
     3421 cgctcatccc ctgaccaaat aacttgataa agttatccag cgtcagggtg tagtcaacgc
     3481 cccagttcac cgtgaagctg ccgtagaaaa tgctgccgta gagcagggcg ttaaaggcaa
     3541 tccataccgt aagtatggcg accacgctcc acaccagggt gaccggtagc ggctggacgt
     3601 cgccacggta ggatttgccg gaaaccgtga cgtaagagcg tttaccaatc cacatatact
     3661 ggacgcagaa caccagcagc gagaacagca gcaggaaagc accaagcgtg ctggcggcct
     3721 gataatccag ctgcgagccg gtaatgtaga agtaaatttg cgtcgccagt acgtcgaagt
     3781 tgccgccaag caccagcggg ttactgaaat cagccagtga ctgcaccacg acgattaaaa
     3841 aggcattggc gagcgcgggc tttagcaatg gcacaaacac gccgttaaag gtttgccagc
     3901 ggctggcgcg cagggtatag gaggcttctt ccagcgaggg atgaatggtt ttaatggccc
     3961 cgtcgaggat cataaaagcc attggcgtga atgccagaac ctgcgccagc cagatcccgg
     4021 taaaaccata cagccagttg gtgttggtca gcccaaacca ctccaccata aactccgtta
     4081 tgtagccgga gcgccccatc atgagcgtca cgcctaagcc gaccacaaaa ggtggcgtaa
     4141 cgatgggcag aatggaaaaa atgcggccaa taatggcgct gcgtttggcg atgcgcgtgg
     4201 tataaatcgc cagcaccagg ccgaagaagg tacagccaat acccaccgct atcgacaaga
     4261 cgatagagtt gacgatcacc tgcacaatat gtgcctgcga gagcacgttc ataaacgcca
     4321 gcggggcgaa ctctcccgcg tcgttggtga acatcgggat aaaaatggcg atgctcggcc
     4381 agacgataaa aatgccaatc agcgccacaa tcgcaatcag tgagccgatc acgaagcggt
     4441 cgccgcccag ccattcgagg cgggttaagg ccagcgtcat aatcgcgcct aattcaacaa
     4501 acagcacgat ggcggcgtag cctaaaccgc gtccagccac ggtggcgctg atgacgataa
     4561 acgccataca gagcaatgcc cagctggcat caaaataatg acgactacgc tgttcgcgtg
     4621 tggcctcacg aaacggacga accagcaaca gactcggtaa taaataccac agccagctga
     4681 tattaaaatg cgaccagcca taagcctcga ggatctcatc actcgtggac tccagcaggc
     4741 cataatcaag gctccagctc ggcaacagga taaatgccag ccagcccagc aaaacccaaa
     4801 ggaaaatcgc atcccgcttt ttcacgggat ggagtgcaag tgtctgtgac atagcagttc
     4861 cggtatggtt ggccggggca ggcgtttgtg ctgcacccgg caaatgcgcc taatgcgggt
     4921 ttatttaccc atcttcactt cgctgaccca tttattaatc aatgccttac gaacatccgt
     4981 ggaaccatat ttatccatgt cgtagttgat taatttcagg tcgtcgagct tcagcgaatt
     5041 tggcgacgtt tcggcggtgg tattggtcag gatctgataa gacttccctt tcttccacgc
     5101 cagttcctga gcttcttttg ataataccca gtccacaaac agtttggcgt tgtcgaggtt
     5161 gcgcgcgcct ttcagaatgc tgacgccgcc aatttcgtag cccgtacctt cacacgggga
     5221 aatcaactcc agcggcgcgc cctgttcttt ttccagcgag tagtcatgca ggaagccaat
     5281 accaatcgcc gtttcgccac gggcggcgtt acgtgccggg gctatgcctg atttggtgta
     5341 ctgggagacg ttggcgttca gttgttttag ataatcaaag gcctgatctt ccccccacag
     5401 ctgcgcaaaa gtagccaggg cggtgtaggc agtaccggaa ctttgcggat cggcaatttg
     5461 aatttcgcct ttatattccg gtttggtgag atctttccag cacttcggca ccggcaggtt
     5521 tttctctttc agacgatcgg tgtttacgcc aaaaccgagg atccccacat atacggcaga
     5581 ggacagattc cctttcactt ttgccggatc gcggaatttc tccatgatct gctcaaggtt
     5641 aggggattta tacgcctgta gcaggcccat ctcgccagcc tgcgattgcg ggtctaaagt
     5701 gccgccgtac cagacgtcag cctgggggtt tttcttttca gcatcgactt ttgccagggt
     5761 gcttccggaa ccgttgcgga taaacgaggt ttttacatca tatttctcgc caaacgcttt
     5821 ggtttcggcc tcacacattt cattggtggc gctgcaatag accaccagcc ggcctttcgc
     5881 ctgcgcggca cccgttaaag tcgccagcgc aacggcagaa gcaatcagag tagaggtaaa
     5941 ggtcattttc attgttttat ccttcgagcg tgagtggtgg tgatgcccgc catcagtaac
     6001 ggcatcagca gtaaacccat aagtactgcg gcgaccgaga gtaaactgaa cattcccgac
     6061 cagccgtagc gttcgatgac cagcgacagt ggccagcccg caagcgccgc gccaaggtaa
     6121 gcgaacaggc cgagaaatcc ggtgattgaa cccgccgcag ctttgtgccc acactccacc
     6181 gccgcaagtc caatcagcat ttgtggacca aagacgaaaa agcccacggt aaaaaagcac
     6241 actgccagca gagcgtagtg atgaaccggt gccagccaca gtgcggcgac tgacaccatc
     6301 aaccccagcg tgaacagcaa aatcattggc gctcgttgcc cgctaaacag caaatccgat
     6361 ccccatccgg caaataacgc gccgagtaag ccgcccactt caaacagcat taccgtggcg
     6421 ttggcgttga gcaggttgac gccgtggctt tccgtcagcc agatattgcc ccagtcgtta
     6481 agcgcaatcc ggatcacata aaccagtaca taagagacgc ccagtagcca gatcagcgga
     6541 ttttgcagca tggtggtgcg caacatttgc cacagcccca tcggcggact ttgttgttcc
     6601 tgacgtaact ccagcggatc gtgacgccag tgaccaactg tcggtaatcc ctcttcctgg
     6661 ggcgtgcctt tgagttgtag cgttaaccag atccctaatg ccatgctgat tatccccggt
     6721 gtcagcatcg cggcctgcca gccccaccag tgggcggcaa aggcgctaat cagcggaata
     6781 atcgctccgc caatgttgat cgacatattc cagcagcccc accagaaacc gcgctcattg
     6841 cgtgagtacc agtgggtcag caagcgggca cacggtggcc atccccagcc ctgaaaaaaa
     6901 ccgttcagtg tccagaccac aagcagaagc gtaagtgatt cgccaaaggc aaacaccacg
     6961 ttcaataacc cggtggcgaa caagccgacg cccataaaac cgcgttgtcc gtggctgtca
     7021 tgccacagtc cggcggtaaa tttcgacaat ccgtagctga gataaaacag cgaacccagc
     7081 aggccaatgt cgcctttatc cagtccgagg tcggtttgca gcgccggaag gacgtaattc
     7141 acgctttttc gcgtcagata aaatgcggcg tagccaataa tcatgcacat cagcaatcgc
     7201 ggacgtaatg tccggtagca ttgattaatt tctctcgctg aacgcgcgtg catggttgtc
     7261 tccatgttgc tgactatatg aaaatgaaag tgaaagggga tgagagaaaa tccgggatac
     7321 ctaagacttt ttcctggtta tccggcgatt tgttgcaaaa atgtgggcaa gttaacaatt
     7381 acccgcgtgc cgtgctgttt ttcgagcgtt aagtcgccgc ccagggcgct gacgcgctca
     7441 cgcatcccct gaatgccaaa tccggccatt ttcccgctgg cgatccccac accgttgtcg
     7501 ctgacctcca gatgcaatac ctctccctgc tggcgcagga taatcgtcac ttcgctggct
     7561 tcggcgtgtt tacagacgtt attgagcaac tcctgcaata accgatacaa ggtaaagcgc
     7621 accgtttcgt tttctggtgt ggagttcagt tgataggcaa actggcaatg aatgccgcgt
     7681 tcactaaagg caaattcatt aatcagatgc agcaacgctt cgcggaatgt cagttcatca
     7741 agcgcgggag gccgcaactg gcgcagcaac tggcgggtgg agagatgaat gcggcgggcg
     7801 agttcattga tttggctggc tgcggattga atttgcgccg gatcgcgcgc ccgcttcacc
     7861 agttgcgact ggatctgaat ggcggtgatg ttctgaccga tttcgtcgtg cagctctcgc
     7921 gccaggtttt tgcgcgtgtc ctcctcggtg tggatcagct tttccgccaa tgcccgccgc
     7981 gctgccagct cggcctccag ccgttgtcgg taatggtgca ggtttagcgc cagatgttgc
     8041 tggcgactga tggcaatgcc caggccaatt cccagcaatg cctgggtcgc gaggaaaatc
     8101 tccagctcca ccaggttact aaagcccacg ccaatctggc gggcgatggt gatcatcatg
     8161 ctgcccaaaa gcccggagag tacgccacct tgccagccaa atttccacgc catcaccaca
     8221 ttgggtagaa aaactacgat caacagcagg cgttcaattt caggtgacag caccatctga
     8281 gtgccgatac caatgataaa aaacaggcta caccagatga ttaaagaggt acgcaagggg
     8341 ggattatgcg tgtcaagccc caggagctgg tagcgatgct gctggcgcag aaactcaaag
     8401 atgagataga caaacggcgt cagtaatacg ccgccggtaa atgaggtcaa ccccggcagc
     8461 attattgggc ctgacataaa aggggagagc agggcggtat tcaacaatga agctgccgtg
     8521 acagtggcta acagcaacgt cagacgctgc cagtaaagtg gaaaacggtg ccagaagttt
     8581 tgcgcaagga tggcaggaaa caggctaata accggcgttg ccagcatgag ataaccgttc
     8641 aatagctgtt cactatggag ccagaacatc atcagcaaag gtgggagcac cagcgcaggc
     8701 cagtagcggc gagagagcaa aataagaagg gcgaggtaaa ccccctgcgg gagaaacaac
     8761 accgcctgtt gaccgttatt ggtcagataa aagctgagcg tccacagcat cagccagcca
     8821 gttccccagg ccagcacaat aaacagagaa atcacccaat gacgaaggtt gcgcgacatt
     8881 aatgccccgc cagcagatgg tggtcgagcg caaaatgcac cagctcaatg gtactatggc
     8941 agttcagttt tcccagcaca ttggcgcgat gcacatgtac cgttttatga ctgagttcga
     9001 gcttaaaggc gatctcttta acgctgtcac ctttgaccag taaatcaaac acttcacgtt
     9061 ctcgtggggt gagaatttcc agcgcctggg cgggttgccc gccgccgcgt aaagcccgaa
     9121 ttgcatcggc acagagataa tgcccgccca gcccgaccga gcgcaccgcc tgtactaact
     9181 cttccggccc acagcgtttg gtcaaatagc cgctggcacc agcatccagc gcgctttgca
     9241 caaaggttgg cgcgtcataa atgctaagaa taatggcgcg aaattgcggt ttttgtgcac
     9301 gcaggcgctt aagcaaactg aggccgtttt cgtccggcat agcaatatca atgacggcaa
     9361 cattgacgtc atcacggatt aatgccggcc atgcctgtgc ggcactactg tactgaccaa
     9421 tgacctccag atcgtcttcc agagagagta attgtgcgaa gccagagcgc accaccacat
     9481 ggtcatccac cagcacaacc cgaatcatac ttttcttctc ttattatctc attcttctat
     9541 catgcatcac ggcgcgtcgc tggcaatcaa ccagcgacag atatggaatg gatgtaacaa
     9601 aaaatgtaat ttgttattca atataattat gctttatttc atgagtggct ctatatctgg
     9661 tgttcagatt attgtctgca ggggcagttc agaagatgtg agcaaataaa aaagaaaaca
     9721 taaataaaac catccggctt tcggggccag atggttattc attattttta tgtcactgat
     9781 tcatcaggtt tgctcagaaa gcgaatcaat gtagtcagca taccactgca tcatttcacg
     9841 gcgaccatcg atatacaggg catgattata ggtacccctg atggaatttt tatccacatg
     9901 agctaactgc atttcaatcc aggcagaatt aaacccctgt tcgtgcagaa tggtactcat
     9961 ggtgtgacga aaaccatgac cggttgccct gccatcgtaa ccgatacgtt tgagcaccat
    10021 attgatgctg gcttcgctca ttggcttgtt gatatcacaa cgtcccggga aaaccagccg
    10081 gtattgtccg gagaaagttt tgagttgttg cagaatgtca gtgacctgtc gtgacagtgg
    10141 tacgacatgt ttacgtcgca ttttcatgcg gctttcaggg attgtccaca gggcattatc
    10201 aaaatcaatt tctgaccatt cagcggaccg tagctcaatc gttctgaggc ctgtcagaag
    10261 cagcagattt gtcgccatgc gggtaacagg actgccatga tattcagcga gcttcctgag
    10321 aaaaacaggg agctcatctg ctctcaggtg ggggaagtgt gcagcttttg gcgccgccag
    10381 cgtactggtc agttcagatg ccggattgac agtggctcgt ccggtggcga tggcataacg
    10441 aaaaatctga ttacagtatt gacgtacctt acgcagtttt tccagcgagc cacgtttttc
    10501 tatgcggcgc agaatttcca gcatttccag cgggaggata tccgtaacgc ttcgtttgcc
    10561 cagatacggg aaaatatctt tatccagcgc ttccagtacg cttctggcgt aactttcaga
    10621 ccatgacaaa gatttatgct ggtgccattc cgtagcgagg gcatggaacg tattggtggc
    10681 agagattttc tgagtgagtt tttcctttcg cttcttttcg cccgggtcac caccttcgga
    10741 gatgattttt cgggcatcgt cgcgtttgat tctggcctca gcaagggaga cgtcgggata
    10801 gacgccgagg gccagttttt tctcttttcc tgctatccgg tatttaagcc gccagtagcg
    10861 ggctcccgtg gtttttacca gcagatataa accacgttcg tcagagagtt tatagtcttt
    10921 ttctttaggt ctggctgttt cgacctgtct ggctgtaagt gccatatggg ggctcctgaa
    10981 actgactgaa cataatgagc ccccaccggt gcccccaatg aaagcttgat tgagaaagag
    11041 ttcagtagag ttcggtggag ttcaggatgt tcagattgct tgtaacgatg tgattatact
    11101 tgagtttcgt ggagttcggg agatgaatct ggaagtgaaa gtggtgcccg gactcggaat
    11161 cgaaccaagg acacggggat tttcaatccc cgtgttgcga gtattcaggt agtgaagtga
    11221 atattctggc tggtttccgt agtgtaagtg attggttgca tactttaaag agtgtgctat
    11281 atgaaaattt taccggttat atccactaaa ggtggcgaag gaaaatcaac ccagtctgct
    11341 aatcttgccg gttttctggc agatgccggc ctccgtactc tgctgataga cggagaccat
    11401 gcacagccca cggcaagcaa tatctttccg ctgacgtatg aagcgccggc cggtcttttt
    11461 gaattactga tgcgcacggc tgacctcagt catcctgaca acatcatttc ccgtacggct
    11521 gtcagggggc ttgacctcat cgtctccaac gacccccatg agcaactgaa aacggccatg
    11581 cttcatgcgc cggatggtcg ccttcgtctg cgtaatgtgc ttcagcatcc gctttttcag
    11641 acctatgacg tcattgtggt ggattcacag ggcgcacgtt ctgtcatgct ggaaat
//
LOCUS       DAZUCC010000156        11644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZUCC010000156 DAZUCC010000000
VERSION     DAZUCC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.157"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..405)
                     /locus_tag="ACHY09_003626"
     CDS             complement(<1..405)
                     /locus_tag="ACHY09_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS3886547.1"
                     /translation="MIVCYVILVNRPFETEIAGKKLFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSV"
     gene            complement(503..696)
                     /locus_tag="ACHY09_003627"
                     /pseudo
     CDS             complement(503..696)
                     /locus_tag="ACHY09_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            complement(1116..1298)
                     /locus_tag="ACHY09_003628"
     CDS             complement(1116..1298)
                     /locus_tag="ACHY09_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886548.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     IYLLQKMNITDKTKYK"
     gene            1237..3324
                     /gene="iha"
                     /locus_tag="ACHY09_003629"
     CDS             1237..3324
                     /gene="iha"
                     /locus_tag="ACHY09_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFS3886549.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKNA
                     DKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSSLYAGDYFQTGSSTTGYVI
                     PERNYWMSLNYQF"
     gene            complement(3542..3692)
                     /locus_tag="ACHY09_003630"
                     /pseudo
     CDS             complement(3542..3692)
                     /locus_tag="ACHY09_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            4186..4428
                     /locus_tag="ACHY09_003631"
     CDS             4186..4428
                     /locus_tag="ACHY09_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886550.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            4719..5087
                     /locus_tag="ACHY09_003632"
     CDS             4719..5087
                     /locus_tag="ACHY09_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886551.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            5091..5303
                     /locus_tag="ACHY09_003633"
     CDS             5091..5303
                     /locus_tag="ACHY09_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886552.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQTFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEAESS"
     gene            5300..5572
                     /locus_tag="ACHY09_003634"
     CDS             5300..5572
                     /locus_tag="ACHY09_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HFS3886553.1"
                     /translation="MTDITDRHTLRRMSWSELTTAAQEAELNRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNPRGRSVCLRPQHPRR"
     gene            complement(5881..6712)
                     /locus_tag="ACHY09_003635"
                     /pseudo
     CDS             complement(5881..6712)
                     /locus_tag="ACHY09_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            6770..6958
                     /locus_tag="ACHY09_003636"
                     /pseudo
     CDS             6770..6958
                     /locus_tag="ACHY09_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(7027..8253)
                     /locus_tag="ACHY09_003637"
     CDS             complement(7027..8253)
                     /locus_tag="ACHY09_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3886554.1"
                     /translation="MKDMQAMLPQTFYIKPGQFIWSYLGTFLFMLGGCIENSWLSAWL
                     NTQGFDQSQIGQIFAAYGIVVAVTSWLSGVCVDMFGPRKVMLAGFSVYLIASIAFLNF
                     AIPSHDFSTILISYMLRGVGYPLVCYSFLVRLTIQLDNHQQGIGTSLFWVVYNLGFTI
                     IGPVVAASLIPVLGHINVMWAGIGVALLGGIFMLVFERNAFILKPRTTSVFKELSAGI
                     SIMYQRPRIGLAVIVKTINGLGTYGFVVVLPLFLLDKQFTLEEWASIWGITFISNQIF
                     NIIFGWMGDKIGFRRTIQIFGSLLTGFATLIVYWVPIVWGHNYVAFMLAMCLWGAGLA
                     GFVPMTPLVPMMAPDKKGAANSAVNFGSGLGNFVGPALVSILTGFGTGVVMYTMAGLY
                     LFSGILVQFLKVPGEK"
     gene            complement(8326..9348)
                     /gene="iolG2"
                     /locus_tag="ACHY09_003638"
     CDS             complement(8326..9348)
                     /gene="iolG2"
                     /locus_tag="ACHY09_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-chiro-inositol-2-dehydrogenase IolG2"
                     /protein_id="HFS3886555.1"
                     /translation="MQKLRCGIIGLGRVGKMHVENMYLLPQLDIVCAADSFIEEMSDW
                     LSSVNITSGYKDYQEMLQRDDIEAVFIFTSTDMHEEIVTAAVQAGKHIFCEKPLSMRE
                     DEKASLAVLRAVKEKGVKLQIAFNRRFDPQFREVYELVRRGKIGQPQMIKITSRDPEL
                     LPHDLIKRIGGLIFDFTMHDFDMARFIMQDEVSEVYVKGNTLIDPTLKNIDDVDTLVV
                     MLTFRNGGYALIDNSRRAVYGYDQRVEVFGSAGMAYADNVSESAVKVYNSQNCIMKNP
                     LPDFALRYREAYRTEILHFIDSVLNHTPIVCTGEDALLAQRIAIAAKQSLKSGLPVKI
                     KTDIQL"
     gene            complement(9521..11461)
                     /gene="iolD"
                     /locus_tag="ACHY09_003639"
     CDS             complement(9521..11461)
                     /gene="iolD"
                     /locus_tag="ACHY09_003639"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFS3886556.1"
                     /translation="MKTIRLTMAQALVRFLDNQYIEVDGREIKFVKGIFAIFGHGNVV
                     GLGQALEEECGQLSVHQGRNEQGMAHIATGFARQMRRQQIYACTSSVGPGAANMITAA
                     ATATANRIPLLLLPGDVYASRQPDPVLQQIEQEHDLTLSTNDAFRAVSRYWDRIIRPE
                     QLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHVPTELM
                     LNDAVALILRHQRPIIICGGGVKYSQSEQELLRFAEQYHLPIAETQAGKGALSSAHPL
                     NVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDV
                     FKLDGVQLQADARVALTQLCERLAPEGYISQWGKTIHRVRSQYLAEVDRVYAAEYSGK
                     DFKPEIVDHMDTQKVFEEFNEITHSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQ
                     RIWQSRGDNDYHVEYGYSCMGYEVNAALGAKLAQPEREVYSFVGDGSFMMLHSELVTS
                     VQMGKKINVILLDNMTNGCINNLQMEHGMDSFFTEFRFHKQDNGRQEGGLIPVDFARI
                     AEGYGCKSYRVTSIGQLLEALEDARKQTVSTLIDIKVLPKTMAHKYLSWWRVGGARVS
                     RSERIQAVARMLDEHIGQARQY"
BASE COUNT         3241 a         2904 c         2589 g         2910 t
ORIGIN      
        1 aacagagttt atacatctga atagcagaaa catgaaacct ctgtgtgtgg aaaaaatgga
       61 aaggcaggca aaaaacatct gctcagaaaa ttgttgtggc aatgtctttt gcagactgag
      121 attctgcatg acattatgta acagagcgat gtgagagaag gttgttttaa tgaacggaac
      181 agaaacagca ctatcagagt gctggatcct gtcggcactt tccatatatt ttctgacaat
      241 gcattcgtca atccggagct cagtaacatt accgggatgg cgcaggaaaa aggactggct
      301 gatgttatct gtcaacagga cgtcattttt ctgaaaaaat agctttttcc cggcaatctc
      361 tgtttcaaac ggacggttaa caagaataac gtaacagaca atcatcacac atactccctg
      421 attagcataa aaacaacgat atcaaaagac tagacgcttt ttcaggaaaa atcttatttg
      481 tccaggatta ttcttacgag accggcgctt caacggtacc gtcacatttt ttacaggctt
      541 tttttacccg ttctgtgctg atcactttca gggagctgct caccagttcc agttgttcag
      601 cgctgacttc cccagataat ccagctcacc gccacactcc gggcaacagc tttcttcagg
      661 ctccagccgg tgtatttcac ggggaaggtg tgccggtgtc agcgctagtg gtataagacg
      721 gttctccctc atcgtttgtc cttagtattg tcagcgccaa caatgtgggc tggcattata
      781 aatgccgtcg aggcaatcgt tcgcctcccc agtgggcaat ccaaatggtg attgtcacgg
      841 ttagacgttt attttgaagg tcgactgaat aaggtgataa cgctgtagta ttttcacgtg
      901 acacagaatt ctgaacgttc tctcttacaa tatttcaatg tttctgtccg tattcaccgg
      961 aagaagtcat cgaccaaatc ataccagtcg tctcactcca ctgaccattc atggttgaca
     1021 ggtggtgaaa atccgcttct gcagtaacca ttttttattc gcaaaaccga acacgccatt
     1081 gcggatggct gttaactggc atgccgaggc agtcattatt tatatttagt tttgtcagta
     1141 atattcattt tttgtaaaag gtaaatataa attattctca ttattgtttg tatttgtgta
     1201 ttgtcttgcc gattaacatg atcggagatt agtaatatgc gaataaccac tctggcttcc
     1261 gttgtcattc cctgtctcgg gttttcagcc agcagcatgg cagcagagga tgtgatgatt
     1321 gtctcggcat ccggctatga gaaaaaactg accaacgcag ccgccagtgt ttccgtaatc
     1381 agtcaggagg aattgcagtc cagccagtac cacgatctgg cggaggcact gagagctgtg
     1441 gagggtgtgg atgttgaaag tggtacgggt aaaaccggtg ggctggaaat cagcatccgt
     1501 ggaatgcccg ccagttacac gctgatactg attgatggtg tccgtcaggg cgggagcggg
     1561 gacgtgacgc ctaacggttt ttctgccatg aatacagggt tcatgcctcc tctggccgcg
     1621 attgagcgta ttgaggttat cagggggccg atgtccacac tgtatggctc tgatgcgatg
     1681 ggtggtgtgg tgaatatcat taccagaaag aatgcagaca aatggctctc ttccgtcaac
     1741 gccgggctga atctgcagga gagtaacaaa tggggcaaca gcagccagtt taatttctgg
     1801 agcagtggcc ctcttgtgga tgattctgtc agtctgcaga tccgcggcag cacacagcag
     1861 cgtcagggct catcggtcac atcactgagc gacacatcag ccacgcgtat tccttatccc
     1921 acggagtcac agaattataa tcttggtgcg cgtattgact ggaaggctac ggagcaggat
     1981 gtgctctggt ttgatatgga taccacccga cagcgttacg ataaccagga tggtcaactg
     2041 ggtagcctga cagggggata tgaccatact ctgcgctatg agcgaaacaa aatttcggct
     2101 ggctatgatc atactttcac cttcggtacc tggaaatcgt atctgaactg gaacgagacg
     2161 gaaaataaag gtcgtgagct tgtacgtagt gttctgaaga gcgataaatg ggggcttgcc
     2221 ggtaaaccgc gggagcttaa ggaatctaac cttatcctga attcattgct gcttacccct
     2281 ctgggagaat ctcatctgct tactgtgggg ggagagtatc agagttcgtc catgaaagat
     2341 ggggttgtcc ttgccagcac aggtgaaact ttccgacaga aaagctggtc agtatttgct
     2401 gaggatgagt ggcatctcac ggatgtactt gcgctgactg caggcagtcg ctatgaacat
     2461 catgatcaat tcgggggaca cttcagtccg cgtgcatatc tggtctggga cgtgaccgat
     2521 gcctggacgc tgaaaggcgg tgtgaccacg ggatataaag cccccaccat gggacagtta
     2581 cataagggga tcagcggtgt atccgggcag ggacgtacta atctactcgg taaccccgaa
     2641 ctgaagccgg aagagagtgt aagttatgag gcgggggtat attacgataa cctctccggt
     2701 ctgaatgcca atataacagg ctttataaca gacttctcca acaagattgt ctcttattcc
     2761 attgatgata acaccaatag ctatacaaac agcggaaagg cccgcttgca tggtgtggaa
     2821 ttcgccggca cattgccgct gtggtcagag gatgtcactc tgtcgttgaa ttacacctgg
     2881 acccaaagtg aacaacgtga tggtgataac aaaggtgcac cgctgagtta tacccctgaa
     2941 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
     3001 gcccgttatc gcgggaaaac accacgtttc acccggaatt attcgtcact gagcgctgta
     3061 cagaagaaag tgtatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
     3121 ggtctgtcgt ggaagatgac ggatgccctg acgctgaatg ctgcggtgaa taacctgctc
     3181 aacaaggatt acagtgacgt gagcctgtac agtgccggta agagttcgct gtatgccggt
     3241 gattacttcc agacgggatc atcaacaaca ggatatgtga tacctgagcg aaattactgg
     3301 atgtcgctga actatcagtt ctgataataa caaaacgcta tcactgacgg tagaatacgt
     3361 tgccactgca actcctggcg gaacagtggc aacgtattag gttaagtgca tttccgatcc
     3421 gctaatgaga tttcgttacc aacaactaat atcgtcacag gaaatgcacg gattattttt
     3481 aacttatcat ttacatactt gtccagagtg taagcgcacc gtgaaggacg tggggtaaaa
     3541 attagtttac agagagagtg acgttccagg gcaacaactc tttcatgcgg ttggcaggcc
     3601 aggtgttgat tacactgatc acgtggcgta gccacgtttc cggctcgatt ccgttaagtt
     3661 ttgcagctac cgatcaggct gtacatcact gccgcactat cgctcgtcat ctcaaagtcc
     3721 ggtctcgtca gcaggaaggt atcattctct cccgccattt ttccgggggc ccggtcagat
     3781 aagtcccttt gtctatcgct gactcctggc tcataacccg gttagcagaa tgcagggtca
     3841 ccacctcgcc acgaccgaat ccggataagt caattgcacc ttctcaatcg ccattttgtc
     3901 acaattaagt ttgataaagc aggtcacctc tgcactgaat ctgctccagt gcctcattgg
     3961 tctccgtccc cccctttaaa ctgccggata agactgctgc acagcgcctt ccccggaatc
     4021 aaatcacttt ccctgtccgc cacaatccgc ttcatgctac ctcgtatttt gtttcagaga
     4081 ccggaatctg ccagaaagtg actacgcgtg cacatatcgg ttttctgggt tacatgcatc
     4141 gtgttatttt ctgaaatgca aaataataaa aaacaaggac acataatgaa ggatgaaata
     4201 cacagtacca gcatcaaccc ggatgttgtt gagcagtttc tggcccttat gaacacacat
     4261 tgtaaatcct cagaagaggc gctcgccatg ttgagtacgc tttgttacaa cctgatggtg
     4321 acatgtggca tccattccat ggacagccag atgctcaatg ggcatgggat atccatgcaa
     4381 cttcagggga ttgatattgc cgaaaggcca ggtaagaata ttcactaacc tgaacagggc
     4441 ctgaaaacgc tgtcttttca ggcctctgca ggcaaaaact gatgacaaca aaatttctgc
     4501 gtttgtaccc caccgatgaa aaagacaaaa actcacaccg ggcttctagc cagtaaagat
     4561 aaaacccgac gtgtcagcct ctacgaaacg ccaacggcat ggtgtatcag ggggcaggag
     4621 tgctacagca aaagtacagg acgccgctgt ggcagccacg acagtctgag cagactgcgc
     4681 ctagacagca ttaagcctgt tgaatgagtg gtggcaccat gaaagataac aacacaaccg
     4741 ccaccccaga agcctggaaa atgcgttttc tggccaacgg gacatccggc acatggtttt
     4801 acactgacga cattgcagtc actgacgcgt ggcttcgcgc gcacccggat gatgtggagg
     4861 ttatccctct ctgccggata ccaccccgtc agcattccca tgaacaacat attgaagaac
     4921 tccgcgcagc cctggcccgg atgctgcata tgcatcatat gatgctggaa accacgcatt
     4981 acagggaaaa catcaccgat gcccgttgta tccgtgaaat gcatgaagct ccattacagg
     5041 cgctcaatat tctggccaga tacccgcata aaaaggacga ggtgtgacgc atgtggctac
     5101 tgattttctg gctttcatct ccctttaatg tctcttccgg tgatgcccgg cagcccacac
     5161 tggcggtgca cagccagaca tttcataccg aacgggcctg tcgcgatgca ttgcgcgcca
     5221 ttaaaaagga gaatgttggc gaattttctc tgcggggcgt ttgtgtttct gacggaaaca
     5281 cgaaggaggc agagagctca tgacggatat tactgacaga catacacttc gccgcatgtc
     5341 ctggtcggaa cttaccacgg cagcgcagga agcagaatta aaccgtgatt acgagcgtgc
     5401 cagaatagtg tggtcattcg ccttacatgt tgcaacaacc acgattaaca aaaatctggc
     5461 catagctcat attcgacgct gcgatacttt gttgcataaa tcaaaaacag tacccggaaa
     5521 caacccgaga ggccggtcag tgtgcctgag acctcaacat ccccggagat gactgacatt
     5581 ccggactgac gcagttgctg catatctgtg gcaatacgtt gtatccagcc agacaggcta
     5641 tcccgcgtag tgacaattaa cccgctttcc tgcaccagct tcagccgggc cgtcggggat
     5701 tttaccagcc ggaaatatca gtaaaacaac atatcattat gccatggtgt acgcggccag
     5761 tgtatgctta cttaaggaat gagggataaa ttggatatta ttacttcact ttgctttttt
     5821 atgagattca tcacataagt ggggttttac tgttaaataa tctgaattta tttgatatcc
     5881 gtaaagcaga tgtctcagtt cactgtttcc aacttttgtt attcctctgg ggcgaatgct
     5941 gtttccgctg gaatattcac ctggcaccag tcccagaaaa gccatgagct gttcaggatt
     6001 ggaaaatcgc gaaaaatcac ccagttcagc aaccagggtt attgcgatta gctgaccaac
     6061 acctttaaga gcctgcaact ggcagaccag attgcacaga gatcactcag gtaacaggcg
     6121 agatatttcc tgatcgagtt gaagtatgcg atcttcaatc tgctccagat tctgacaata
     6181 atgttgaaag caatctgttg agatagcaga ccaaatgatt gattaactaa ccaggttcga
     6241 tgtttgccgg ttcagtgctt cctggcataa cgtttgtctg ttcgtgtcac gttttgcagc
     6301 ggcacgggct ctgattaaat cccgcatagc ttcatgagtc agctcaggga gccagactgg
     6361 gggaacctca ccatccctca gtaacctggc gagagaaatt acgtcccgat ggtcattttt
     6421 gatcctgtct gccggtgatt tgggtatacg ggaagggggc aataaccagg cattttatat
     6481 tcatcactgt gagttgatga tatagcccaa acccacaggg accaccttca tagcaggaaa
     6541 gaatgtttcc gtaaggtccc tgcaatttct gacataatcg atgtacagcc aaggcttcgt
     6601 tgttaatcgt accataataa cgaacttcgc caagccattc aggtgtagca atcgccaagg
     6661 tttctttatg aacatccagc ccgacgtaag ctgcaatatt ttccgaatcc atgatgacct
     6721 ctggagtatc ccaccaatgc tatagtgagt atataaaggt catatcatct acgtgttctt
     6781 cggcaacgat cacagtggag agcgtggagc gctactgtac gggctgattg gcacctgccg
     6841 actgaacagt atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg
     6901 gccttccaac cgtgttgacg aactgctgcc atggaacgta gttctcaccg ataaataagc
     6961 gtcaatacgg cattcgatta atgcacttac agctaaatgg agtttttgaa caatatttga
     7021 cgttatttat ttctcaccag gaactttaag aaactgaacc aggatgccac taaataaata
     7081 gagtcctgcc atagtataca taaccacgcc tgtaccaaaa cctgtcagaa tggataccaa
     7141 cgcaggtccg acaaaatttc ccaggccaga accgaaattc accgctgagt ttgctgcccc
     7201 cttcttatcc ggtgccatca tcggtaccag cggcgtcatc ggcacaaatc ccgccagccc
     7261 cgctccccac aggcacattg ccagcataaa agcaacgtaa ttatgccccc acactatcgg
     7321 cacccagtag acaatcagcg tggcaaaccc ggtcagaaga ctgccaaaaa tctggatagt
     7381 acggcgaaaa cctattttat cccccatcca gccgaaaata atgttgaaaa tctgattaga
     7441 aataaaggtt ataccccaga tactcgccca ctcctcaagg gtaaactgtt tatccagcaa
     7501 aaacagcggc aacaccacta caaaaccata ggtgccgagt ccattgattg ttttaacaat
     7561 cactgccaga ccaattcggg gtcgttgata cataatggaa atacctgccg acaactcttt
     7621 aaagactgac gtagttcttg gcttcagaat gaacgcattg cgctcaaaga ccagcatgaa
     7681 aatcccccca agcagtgcaa ccccaatccc ggcccacata acattaatat gcccaaggac
     7741 aggaattaac gaagcagcta ctaccggacc aatgatggta aaacctaagt tatatactac
     7801 ccaaaacagc gacgttccaa ttccctgttg atgattatcg agttgaatgg tcagtcggac
     7861 aagaaaggaa tagcagacca acggataacc tactccccgc agcatatagc taatgagaat
     7921 tgtactaaaa tcatggctgg gaatcgcaaa attaagaaac gcgatagaag cgatcaggta
     7981 aacggagaaa ccagcaagca taacctttct ggggccaaac atatccacac agacaccgga
     8041 taaccaggaa gttacggcaa ccacaattcc gtaggcggca aatatctgtc cgatctgaga
     8101 ctgatcaaat ccctgagtat tcagccaggc agatagccaa ctattttcaa tacagccgcc
     8161 caacataaat aaaaatgttc ccaggtagct ccagataaac tgacctggct ttatataaaa
     8221 tgtctgaggc aacattgcct gcatatcttt cattttataa ccctcagtac ggataatctc
     8281 tcgctcgtaa tagtgcgaga gacccttcgc tatttacaaa tgcaactaaa gttgaatatc
     8341 agtttttatc ttcactggca gtccactttt taatgattgt tttgcagcaa tggcaatacg
     8401 ctgcgccaat aatgcatctt caccagtaca gacaatgggt gtatgattca gaacagaatc
     8461 aataaagtgt aatatttccg ttctataagc ttcacgatag cgtagcgcaa aatcaggcaa
     8521 aggatttttc atgatacagt tctgactgtt atataccttc accgccgatt cactgacatt
     8581 gtcggcatac gccatccctg ctgaaccaaa gacctcaacc cgttggtcgt agccataaac
     8641 cgcccgacga ctattatcaa ttagcgcata accaccgttt ctgaaggtca gcatcaccac
     8701 gagagtatct acgtcatcaa tatttttcag agtaggatca atcaatgtat tgcctttaac
     8761 ataaacctcg ctaacttcat cctgcataat aaagcgagcc atatcaaagt catgcatagt
     8821 aaaatcaaat atgagtccac ctatcctctt gatcaaatcg tgcggcaaca actctggatc
     8881 acgtgaggtg atctttatca tctgaggctg accaatcttg cctctgcgca ccagttcata
     8941 cacctcccga aactggggat caaaacgacg attaaaagca atctgcagtt tgactccttt
     9001 ctctttaacc gcccttagca ccgcaaggga agctttctca tcttctctca tgctgagagg
     9061 tttttcgcag aaaatatgtt tacccgcctg gactgctgcc gtaacgatct cttcgtgcat
     9121 atctgtcgag gtgaaaataa acaccgcctc aatatcatca cgctgtaaca tttcctgata
     9181 atctttatat cctgaggtta tatttaccga actcagccag tcgctcattt cctcaataaa
     9241 actgtcagca gcacagacaa tatctaattg aggaagtaaa tacatattct caacatgcat
     9301 tttccctacc cggccaagac caataatccc acatcgaagc ttttgcattt atacctcgtc
     9361 atatcatgat atagtaaata taaaacgaca aaagaaaatc ccctttatcc aacacggtac
     9421 gaaagacttt attttgtagt ttcaacgaac aaatactttt accgtaaaaa acgtcaggtt
     9481 gcagaaaggc gccctcctgc aaccttaaaa actgaattaa ttagtactgt cgcgcttgtc
     9541 caatatgctc atcaagcata cgagctactg cctggatgcg ttcgctacgc gacacgcgag
     9601 cgccaccaac acgccaccag ctcagatatt tatgcgccat tgttttaggc agtactttaa
     9661 tgtctatcaa cgtactcacc gtctgtttac gtgcatcctc cagagcttca agcagttgtc
     9721 caatgctggt gacgcgatag cttttacagc catatccttc ggcaatccgg gcaaaatcca
     9781 caggaatgag tcccccctcc tgacgaccgt tatcctgttt atggaaacga aactcggtaa
     9841 agaaactgtc cataccgtgt tccatttgaa gattatttat acaaccatta gtcatgttat
     9901 ccagcaaaat aacattaatt ttcttcccca tctggaccga agttaccagc tcagaatgca
     9961 gcatcataaa tgagccgtcg ccaacgaagc tatatacctc ccgctccggt tgcgccaact
    10021 tcgcccccaa tgcggcattg acctcatagc ccatacagga atagccatat tctacgtggt
    10081 aatcattatc gccacggctt tgccagatgc gctgcagatc gcctggcaga cttcccgccg
    10141 cagcaaccac cagcgcgttt tctggcagca ttcgatttag tactccaaga acgcgcgtct
    10201 gtgtcagcca tgaatgagta atttcattga actcttcaaa cactttttga gtatccatat
    10261 gatccacaat ttccggtttg aaatccttac cgctgtattc cgctgcatag acgcgatcca
    10321 cttccgccag atattgagaa cgaacacgat ggatcgtttt cccccattgt gatatgtaac
    10381 cttccggagc cagtcgttcg caaagctgcg tcagggcaac acgggcatca gcctgcaatt
    10441 gcactccatc cagtttgaag acgtcaaaac ggctgacgtt tatattcaga tagcgcacat
    10501 caggattctg gaaaatccat ttagaagagg ttgtgaaatc ggtataacgc gtacccacac
    10561 caataatcag atcggcctcc tgagccagca aattcgccgc cagcgatccg gtttcaccaa
    10621 tcccaccaac attcagcgga tgcgcagaac tgagcgcacc tttaccagcc tgcgtttcag
    10681 caatcggcag atgatattgt tcggcaaatc gcagcagttc ctgttcagac tgcgaatact
    10741 tcacaccccc accgcaaata ataattggtc tctggtggcg aagaataaga gcaactgcat
    10801 cattcagcat tagctctgtt ggcacgtggc gctcaagacg atagacccga cgtgcaaaaa
    10861 aatagtctgg ataatcccag gcttctccct gaacatcctg cggcaaacaa agcgtcacag
    10921 ctcccgtatc agccggatcg gtaagcaccc gcatcgcgct aatgcaggcg ctcattagct
    10981 gttccggacg gataatgcga tcccaatagc gactcactgc acgaaaagca tcattggtgc
    11041 tcagcgtcag gtcatgctcc tgttcaatct gctgcaacac aggatcgggt tgacgcgatg
    11101 catacacatc tcctggtagc agaagcaacg ggatacggtt cgccgtcgca gtcgccgccg
    11161 ccgtgatcat attagcggca ccaggcccta cggaggaggt gcaggcataa atctgctgac
    11221 gacgcatctg acgcgcaaat cctgttgcta tatgtgccat accctgttca ttgcgtcctt
    11281 gatgaacgct aagttgacca cactcttctt ccagagcctg ccctaatccc acgacattcc
    11341 catggccaaa aattgcgaaa atacctttta caaacttgat ttcgcgaccg tccacttcaa
    11401 tgtactgatt atcaagaaag cgcaccaaag cctgcgccat agtcaacctg attgttttca
    11461 tgcactccac ctatcttatc aaccgtttga ctgtcgttac ctacgtacgc actgctacag
    11521 ccccgggcat aaaacactca ccacaagaaa aaaattccgc aaacgcaatt atatgaaaca
    11581 tatttttcat ttgtgagaag aagatcaaaa aacgattaaa aatttcgaga atagctaaaa
    11641 tacg
//
LOCUS       DAZUCC010000157        11640 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZUCC010000157 DAZUCC010000000
VERSION     DAZUCC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.158"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..719
                     /locus_tag="ACHY09_003640"
     CDS             <1..719
                     /locus_tag="ACHY09_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFS3886557.1"
                     /translation="ALFALPFMASATASELSIGAGAAYNESPYRGYNENTKAIPLISY
                     EGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPTDNDDYAMQQLDKRDSTAMAG
                     VAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGVLYYDENFS
                     DYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYSELPEEITD
                     SPMIDRNESFTFVTGVSWRF"
     gene            complement(840..2819)
                     /locus_tag="ACHY09_003641"
     CDS             complement(840..2819)
                     /locus_tag="ACHY09_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFS3886558.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNAYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHTVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKT
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHCHPEVG"
     gene            complement(2830..4230)
                     /locus_tag="ACHY09_003642"
     CDS             complement(2830..4230)
                     /locus_tag="ACHY09_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3886559.1"
                     /translation="MTSTPITAADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTTSSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            4456..5271
                     /locus_tag="ACHY09_003643"
     CDS             4456..5271
                     /locus_tag="ACHY09_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3886560.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKASDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(5303..5998)
                     /gene="araD"
                     /locus_tag="ACHY09_003644"
     CDS             complement(5303..5998)
                     /gene="araD"
                     /locus_tag="ACHY09_003644"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS3886561.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDEARQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(5992..6852)
                     /locus_tag="ACHY09_003645"
     CDS             complement(5992..6852)
                     /locus_tag="ACHY09_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS3886562.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(6845..7507)
                     /gene="ulaD"
                     /locus_tag="ACHY09_003646"
     CDS             complement(6845..7507)
                     /gene="ulaD"
                     /locus_tag="ACHY09_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS3886563.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGA
                     RA"
     gene            complement(7504..9000)
                     /gene="lyxK"
                     /locus_tag="ACHY09_003647"
     CDS             complement(7504..9000)
                     /gene="lyxK"
                     /locus_tag="ACHY09_003647"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFS3886564.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPLVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTTQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRVHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(9004..9990)
                     /gene="yiaO"
                     /locus_tag="ACHY09_003648"
     CDS             complement(9004..9990)
                     /gene="yiaO"
                     /locus_tag="ACHY09_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFS3886565.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGAELLKAVDEVQ"
     gene            complement(10003..11280)
                     /gene="yiaN"
                     /locus_tag="ACHY09_003649"
     CDS             complement(10003..11280)
                     /gene="yiaN"
                     /locus_tag="ACHY09_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFS3886566.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(11283..11639)
                     /locus_tag="ACHY09_003650"
     CDS             complement(11283..11639)
                     /locus_tag="ACHY09_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFS3886567.1"
                     /translation="MSRYLFVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALV
                     THSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLY
                     QLITRSNSLTSPPQGA"
BASE COUNT         2774 a         3129 c         3124 g         2613 t
ORIGIN      
        1 tggcgttatt tgcgttgcct tttatggcaa gcgcaactgc ttctgaatta tccattggtg
       61 ctggtgcggc ttataatgaa tcgccttatc gcggttataa tgaaaataca aaggcaattc
      121 cgctgattag ttatgaaggt gatacttttt atgttcgtca gaccacgtta ggttttattc
      181 tgtcgcaaag tgaaaaaaat gaacttagcc tgaccgcatc ctggatgccg ctggaatttg
      241 accctaccga taatgacgat tatgccatgc aacagcttga taagcgtgat agtacggcta
      301 tggcgggggt tgcctggtat caccacgagc gttggggaac cgtgaaagcc tctgcggctg
      361 cggacgttct ggataacagc aacggctggg tgggggagct atcggtattc cacaaaatgc
      421 agataggtcg cctgtcgctg acacctgcgc tgggcgttct ctattatgac gagaatttca
      481 gtgactatta ctatggcatt tcagagagtg agtcccgtcg tagcggtctg gcaagttatt
      541 ccgcgcagga tgcctgggtg ccttatgtca gcctgacggc aaaatacccg ataggagagc
      601 acgtcgtatt gatggcgagc gccggataca gcgagctgcc ggaagagatt accgacagcc
      661 cgatgattga tcgtaatgag agttttacct ttgtcaccgg cgtgagctgg cgtttttaat
      721 ttaccggtgg atgtcggtgc ggctggccta cgctctcgca ccgactcgtg ggcctgataa
      781 gacacgttaa gcgtcgcatc aggcaatgtg ctccctacgt tggatgcggc gcgagcgcct
      841 tatccaacct ccggatggca atgcttttcc tcattcaccc agatccgcat ttcgccttca
      901 ccccggttgg cccagctaaa ccacgggata aaggtcaggg tttgcggctg gcgtttggct
      961 ggcgcgctgt cgtaatgcca cagcggttgc tgctctggat tgctctgttc ataccgataa
     1021 cccggtgcct ggattaagat cttatggcgg aacaatccct taccttcaaa tgtcgtaaat
     1081 ggcgcatcgg cgggcagcca cagattatgc agtgactcgc cgttgtcggc ctgttccagg
     1141 caatacacca gcgggccgcg ctgaatcgcc acttttccgg cgacgtgacg caccagaggg
     1201 ttaccgtaaa cgcgacgtac cggcattggc aaagtcagat tcagcgtgtc gccctcctgc
     1261 cattcgcggg taatgtgcaa atacccttta cgaatatcct gctcgacctc ttccccattc
     1321 aatatgatct gcggctgtgt gcaccagtcc ggcagacgta aagccagcgt atgacgcacc
     1381 ggctggggcg attcaaccgc aattgtcacc tgttcctgcc acggatagtt tccgctaacc
     1441 cgcagacgca gcgtgccatt ttctaccggc acttccatgc tgtttcctgc gtagatgttg
     1501 atatacaacg catcttcacg cggcgtgtag agataatgac caatcgaggt tagcacgcgg
     1561 gcgatatttg gcggacaaca agcgcaacca aaccaacgct ggcggatcgg cttaacgtga
     1621 tcgtagatat ggttgaattt taacgttttt ggatgcactt ccagcggatt aacgtagaag
     1681 aaatgtttgc catccagcgc catgccgccg agcacagtgt tgtacagtgc gcgctccatc
     1741 acatcggcat attgactgtc gccttccatt tccagcattc gccgggcgaa catcatcagg
     1801 ccgatggaag cacaactttc ggcgtaaacc gtgtcattcg gcagatcgta atcgctgctg
     1861 aacgcttcgc cgctgctttg tgagccaatg ccgccggtaa tatataactg acgctgggcc
     1921 atattgttcc acagccgcag gcaatcctga cgcttgcttt catcgtgact taaacgcgcg
     1981 agatgtgcga cgccagtcat cagatataca aagcgtactg tatgtccgat ggcggtttgt
     2041 tgctgtgcga tgggcaaatg cgcctggctg taggctttat ctttcaccat ccacgccggg
     2101 ccgtaggtgt gccagtgcga ggtctgcccg cgcttttcat attcctggtc gtaatagtgc
     2161 ggttgcgcac cacgctgttc gacaaaatag ttcgtcagcg ccaggtagcg cggctcttcg
     2221 gtcacttcat acaggcgcat tagtgccagt tcaatttccg ggtgaccagg ataaccgtgt
     2281 aacttacttt cacctggacc aaatacgctg tcgatatgat cggccagacg gcaaaccact
     2341 tccagcaagc gccgttttcc cgtggcctgg aagaaggcga ctccggcttc aatcagatga
     2401 ccggcgcagt aaagttcatg acactccgcc agattgctcc agcgttcttc gggcgctttt
     2461 accgtaaagt aagcattgag atagccatct tcgcactggg cggaggcgat cagttcaatc
     2521 acctcgtcag cggttttttc cagttcggca tccggcttct ggcacagcga ccaggctacc
     2581 gcttccagcc atttggcgac gtcgctgtcc tgaaacacca tcccgtaaaa ttcaccgtct
     2641 tgcagtcctg cggcaatgcg aaagttttca atcgcatggc tgggttccgc ttctgggata
     2701 cgatcgttca aggcgtccca ctgataagga atcaccacat cgcggaccag ttgttggtac
     2761 tgaccgagga acggatcgct gaccgtcagt ttatgcagat cgacttccga aatgttcatc
     2821 ttgtgctcct taaggctgta catgacgctg acgcaggtcg ctagcaatgc gtgacatcat
     2881 agggttattg agtttgcaaa agcggatcgc ccctgccagc agcaggtgga atagcgcagg
     2941 cagtaatgtt tccatcgccg tgatcccgcc aagcgaactg gtggtctggt tttccacgcc
     3001 tggctggtag gcgacaaaaa taaacagcag gctgatgatc ccggcgctgg aggcccatgc
     3061 cagcttgatg aaaaacagat tgaaggcgaa gttcatgccg gaagaacgta cgcgggtttt
     3121 ccactcgccg tagtcatcgg caaaggccat caatgagaag tgcagtggca atgtgaagcc
     3181 aagaatcacg ccattaccga ggatcaccgc cagccacagc gtttgataag ccgggccaga
     3241 aggcaggaac cacatcaaca ccgccagcgc agcgaggagc aggttggtgt agtagtaaat
     3301 tttgacggta tcgaaacgcc gcgttaacgg gctgacaatc accgagccga taatggaggc
     3361 gaaggtgacc atggtgaaga acagcgacgt gtagcccgta ctgccttgta agacgtaggt
     3421 aatgaagtac atatacccac cgccgcgaat attaaagacg ttaatcagca ggaaagacat
     3481 gaccagcatc agcagcagtt ggtcgttgtt ccgcagcccg gcaagatgtt cgcgcagggt
     3541 aaatttcccc attgtggaga gtggcacccg ttcacgaacc cagaagaaac agcacaggaa
     3601 catcaccacg gcaatggcgc acagcacgcc gacgcccagt tgatagccgc gcgcggcatt
     3661 accctgaccg aagagatcca ccatccacgg caggccaacc gaaaccagaa atcccgctac
     3721 gccgcagaga acgaatcgcc aggactggca ggagatcact tcattgtggc gggtggtcat
     3781 ggtgttgatc agcgcgcagt acggtacgtt gatggcggta taaccgactg aaaggagcag
     3841 gtaagtaccg aacgcccagg cgatttttac tcccatgctg gcatctggca cggtgaacgt
     3901 caggatgccg ataatgccga tgggaatggc aatccataac tgccacggac gaaagcgccc
     3961 ccagcgactt tgcgtccggt cggcaatgac gcccatcacc gggtcggaga tggcatcaaa
     4021 cacgcgcagt gcaataaaca gcgtgccgac cagcgccgga gttaagccaa aaacgtcggt
     4081 gtagaaaaag gtgagaaagt tcatgatcag gcaagttatt acggtgccgc ctgcgtcacc
     4141 caggccgtag cctatttttt cccgtacaga taacgcatcg tcaattttac ctttagcgat
     4201 atcagcggca gtaatcggtg tagaggtcat agatattcct cgtgaatagg gagcaatttt
     4261 tagttttatt gcaatgacct gattaattct gccgtgaacg ggaatatcaa caaggcgaga
     4321 aaagagtaaa aaaaggtgac gattccgacc tgaatttaaa agtgtgaaat ggatcaggtg
     4381 aaattaatgt ttttgattaa taatcaaaag gtaatgtgtt taatcattaa tgaataagcg
     4441 ggaaattaca tatcaatgct cgaattatcc atgacgttac cgattaaagt gcaaaatggt
     4501 ggattattta tttcccgtgg cgtagggagg cacccggcaa ggcggctgga gtcatgggaa
     4561 attatctttg tcgaaaaagg tcgattaacg attcaggaag aagagcggat atttctggtc
     4621 gatgcgggag agagtttatt actctggccg aaccgacagc atgttggggt agaggatttt
     4681 cccgccgatc tcaaatttta ctggctgcat tttgaggtta aagcgtcgga tagcgacccg
     4741 cgctggctca ctcatcttag cgtgccgcaa caaacgaagg ttgccgatcc gcaggcgctg
     4801 gtggccctgt ttcgccagtt cttaagcgaa caggaaaagc atcaacgcac tccggcgctg
     4861 gagtttattg tgctgctgat tttgcagcag ctcacccgtg acgcgcgtct tgatgaacag
     4921 gccgatgccg caggtaccac gcttgcctgg aaagcgcaga aactgattcg cacgcaatat
     4981 catcgcccgc tttctacggc gcttttagcc cgcgagctgt actgcaatgc cgattatctt
     5041 gggcgcgtct accggcgggt gtttcatctg acgttaacag aagcaattca tcgccagcga
     5101 gtgcgcgaag ccgaaaagct gttaattagc gaagcgcgat cgctcactga agtggctgcg
     5161 ttgtgcgggt ttaatgacgt tggctatttc cgacagattt ttcgcaaaca caccggttta
     5221 acgccagctg cgtggaagcg acgttacagc aaggaacata tcaattcgta gtgccggggc
     5281 aatgaagccc cggcgtgagg gattactgcc cgtaataggc attggcccca tgcttacgca
     5341 ggtagtgctt atctaacagt tcgttttgca tcgcagggag ctgcggcgca agctggcgcg
     5401 agaacaggcc catataggcg cactcttcca gcactacggc attatgcacc gcatcggcgg
     5461 cattttttcc ccaggcgaac gggccgtgag aatgcaccag caccgccggg atttgtgccg
     5521 gactcctgcc gcgttcttcg aaagtttcaa tgatcacttc gccggtctga tattcatatt
     5581 cgccgttaat ctcctctgcg gtcatctgtc gcgtgcaggg gatggcacca tagaaataat
     5641 cggcgtgcgt ggtgccccag gcggggagat ccagcccggc ctgcgaccag atggtggcgt
     5701 ggcgcgagtg ggtatgcaca atgccaccga tttcggcata gcgacggtaa agcgccagat
     5761 gcgttggcgt gtcggacgag ggctttttgc tgccttccac caccttaccg ctggctatct
     5821 caaccaccac catatcgtcg gcggtcatca cgtcgtactc gacgccggaa ggtttgatta
     5881 ccatccattg ccgcgcttcg tctaccgcgc tgacattgcc ccaggtgaac gtcaccagat
     5941 ggtgagcggg aagtgccaga tttgccgcca gcacgtcggc tttcagttgc tctaacatat
     6001 aaaacctgcc tcctgcatac gcgcttcaat ccagcgccgc gcctgaataa tctccagcac
     6061 cggctctttg gctttttcgg tccacatctc aatcaggaaa gaaccgcgat agttcagttc
     6121 atgcagcgtt ttgaagatgc caacgaaatc gacgcagcct tcgccaaacg gcacatcacg
     6181 gaactgtccg gggctttgct cggtgactgg ttgagtatct ttcaggtgga tcgcggcgat
     6241 acggtcaatg cccagtttca gttcggcggg aacatcattg ccccaggcgc tgaggttgcc
     6301 gacgtccggg tagacggtga accacggcga ggcgagcatc tcgtcccact ttttccattt
     6361 gctgatggag ttcataaacg cagtatccat aatctccacc gccagcatta cttgtgatgc
     6421 cgccgcctgt tcgactgtcc acgccagccc ttcagcaaaa cgttgccggg tgccttcgtc
     6481 gtggtcttcg taatagacgt cgtagcccgc cagctggatg gtgcgaatgc cgagatcgcg
     6541 cgccaggcga atcgctttac tcatgatttc ccgcgcccgt tcgcgcactg cctcgtcacg
     6601 gctaccaaaa ggaaagcgac gatgtgcgga caggcacatc gacgggatcc caacacccgt
     6661 ttcgatcatc gcagtgacca gcgaagcacg ttgtgcggcg ctccagtcaa gacgtgagag
     6721 ccgttcgtcg gtttcatcca ccgacatttc gacaaaatca aagccacagc tttttgccag
     6781 caccagccgc tccggccagg agagatcttt cgccagcgct ttttcataaa tccctaacgg
     6841 atgattacgc acgcgcacct ccccagatgg catcaatttg tgcatggaaa tcgccagcca
     6901 cctgcaccgg atttgctgcg cctgccagtg ctcgcccggc aataaacgct ttcacgcgaa
     6961 tatctttaaa cagcggcagg tcagcagggg taatcccgcc ggtaatggaa agctcaaggc
     7021 cgatatcgga aagcgccttc atgcgtgcca gatcggcctc gccccactgt tgcccgctgg
     7081 cctgcgcgtc gcgaccgcga tgataaatgg cctgccgcac gccaatacga tgccagtcgc
     7141 gggcgtcgtc cagcgtccag ttaccgaaca actcgatctg aatttcacca ccgcagcgtt
     7201 gcgccatcgc gtggcctttt tccaccgtcg cgagcggtgc ggcgcagatg atggtcatcc
     7261 agttagcgcc tgcgccaaac gcctgttgcg cgagcgtttc accggcatcg gcgaccttcc
     7321 agtcagcgac gatgattttg ttcgggcact gttcgcgcaa ggctttcact gcgccaagcc
     7381 cttcgtttaa acagagaatg gtgcccgctt cgacgatatc gacgctgtct tttaacagcg
     7441 tcacgtcgcg ctgtgcgtct tcaagtgatg agtggtcgag ggccagttgc agaagtggtc
     7501 ggctcataat gtgtgctcct tgatgcgggc gtgaaagccc tgaagtgcgg caatgagatg
     7561 ctgataacgt tggtattttt gttggtaaag ctgatgggcg gtcatatccg gcagcagggt
     7621 gcgtaccggg tgctgtaaat cacgttgggc ttcgctgaaa tcgcgataaa ctccggtgcc
     7681 gacgcgggcg gcaagtgccg caccaaagca gccggtttct tccacctgcg gcagctcgat
     7741 acgcagaccg ctgacgtccg ccagcatttg catccagaca tcggagtgcg ccggaccgcc
     7801 agtgacgcgc agggtgtgca catcagtaaa acgttcgcgc atccggttga gatgggtcat
     7861 atggctgaac accacgcctt cataaatggc ctgcaacagg tgtacgcggg tgtgaatggc
     7921 ctgcatcccg tagaaaccgc tggtcatctc gagtccagcg ttgctgccgt acaggaacgg
     7981 caggaaaaag agatcgcccc cggccttcgg caagctggca acggcctggt tgatctcatc
     8041 aaacgagatt tctccccact gtgtggtaaa ccattcgaga ttgccggaag aggtggggct
     8101 tgcttcatga acgataaatt gaccatcgtt aacgtagcga ccatagacat acggatgcgc
     8161 ttcaccgtca cgtaaaccac gggttatgcc gctggtcacc gcccaggtgc ccatcaccgc
     8221 attgagggta aattcgtctt cgatcccggc gcagagtgcg gtggaaacca catcaaacag
     8281 gccgccgaca acgggcgtac ccgctttcag accggtcagt gcggctgtct gagcggtgat
     8341 ctccccgcag atttcggcag atccgacaac aagtggcagg gcgtggttga tttcagcgat
     8401 ccccagccag tcggtgaggc acgggtcata ttccccaaga ctcatgttgt agagattgga
     8461 ctcagaaata ttgctctctt cgcaaccttt gacgccggtt aaacaccagc gcaggtagtc
     8521 gtgcgtcatc atcacgcagc caatttgcgc gtagcgttcc ggttcgtgct ctttcagcca
     8581 gcgtaacagc gacaccggat gcccggtcca caaggtttgt cgcgtcagcg ggtagagttt
     8641 ttccgggatg ccatcttcct gccagcgacg aacgatttcc atcgcccggc gatccgagga
     8701 caaaatggcg ttaccgagcg gcttgtcgtt tttatccagc aaaaacaagc cctttccctg
     8761 tgcggagatg ccgataccga caatttgttc cccgctaaca ccagaatgag taagcagggc
     8821 gcgaatgaca gccatgcagc attgccacag ttctgccata tcgcgctctg cccaacctgg
     8881 ctgcgggctt aatgcgcaca gcggcaggcg ctgcacgcct gcctcccggc cttcgcggtc
     8941 atacagcccg gctttcagcc agctaccgcc acaatctaac cccagccagt attgcgtcat
     9001 ggtttattgc acctcatcca cggctttcag cagctcagcg ccctgcggaa catctttaac
     9061 aaacatgtcg cggacctgag tacccagtgc gtcgctaaag gctttgcggt cgagatcggt
     9121 gatgacttcc acgcccgctt ctttcatgcc gtcgatgatt ttttgctgat cttcggcaac
     9181 cagtttgcgc tggtagttac ccgcttcctg tgcagatgaa actagcgcct gctggaactc
     9241 cgggcttaag ccatcaaact tcgctttgtt gatcaccacc agaagtggcg aataggcgtg
     9301 gtgcgtcaga gaaaggtact tctgtacttc gaaaaatttt gctgaccaga cgacgttgat
     9361 cgggtgttcc tgagcgtcga tagtgcgcgt ttccagcccg gtatagactt cggcaaacgg
     9421 catcgggatc gggttagcgc caaagacttt gaatgcggcg atattcatcg ggctattgtt
     9481 ggtgcgaatt ttcagccctt tcaggtcggc gggggtttta accggtgcgc gcgagttggt
     9541 gacatcgcgc cagccgtttt cccagtaggc cagtactttt agtcctttac cttcaagtga
     9601 ggctttcaga tcatcaccga ctttgccgtc gagcgtttta tgcgcgtgag cggtatcgcg
     9661 gaacaggaaa gggacatcaa gcaagttcat cactggtgat aacccggcaa agttattcga
     9721 gccggacatt tccatatcga tggtgccgcc acgtacgccg ctgatcatcg cctgtgcgtt
     9781 accgagagta ctgtccggga acagttttaa tttcagctcg cctttggttc tctcctgcaa
     9841 taaatcattg aattttttcg ccgcaatatg ttgcgagtcg gtttgtgagg tttcataacc
     9901 gaaacgtaaa gattgcgccg ccagtgaaga tgtactaaat gcagccaggc cagcaatgaa
     9961 taatgcgtag gttacagagc gtaatttcat aatattttcc ttttaattaa tccatttcaa
    10021 agggaggatg atgagatcgg gaataaaaac aaatacgact aataatgaat ataaaaccag
    10081 gacgtaaggg aaaacgcctc tgactgcatc atcgaatttg agttttgcca cccctgaaat
    10141 aacgttaagc acattaccga taggcggtgt aattaagccg attgagcagt taatgataaa
    10201 catgacaccg aaataaatcg gatcgattcc tgcctcttta actaaaggca ttaataccgg
    10261 ggtaagaatt aataccgtcg gcgttaaatc catgaccatg ccgacaatca ggatagccac
    10321 cataatgacg ataaacagca gacgcggtga atcgaccagc ggttgcagta aatcagaaac
    10381 catcatcggc agttcagcaa tggtgatcag ccaggcggaa acttgtgccg aggctaccag
    10441 aaacatcacc actgaggtcg ttttcgctgc gccaatgagc acatgccaaa gtgtggcgaa
    10501 ggtcatttca cggtaaataa ctgtggcgac aaacagcgca tagaaagcag caacggcccc
    10561 ggcttcggta ggggtaaaca gaccggagcg aaacccgcca ataatgatga ctggaagaaa
    10621 cagcgcccag ataccggaga caaaagagtg ccagatttcc tgcatcgttg ctttttgctg
    10681 gcggggtaaa ttaaggcgac tggcctgcca ccaccaggta agcatcagcg ttgcgcccat
    10741 catcatgcca ggggcaatgc ccgccataaa cagcttactg atggataatc cgctggaaac
    10801 gccgaaaata ataaacggaa tggaaggggg aataattggc gcgataatgc cgccagaggc
    10861 aatcagcccc gcagcccggt tgaccgggta gttagcgctg cgcatcatcg gcactaatag
    10921 cgcggcaacg gcggcggtat cagcaacagc agaaccagaa aggctcgcca taatcatagc
    10981 cgccagcacg ccgacgtagc ccaacccgcc aggtttatgc cccaccagtt tcatcggcag
    11041 gtcaacaatg cgttttgaca agccgcccgc attcatgatt tcacccgcca gcacaaagaa
    11101 cggaatcgcc agcagggaga agctgtcggc tccgttcacc agcgtttgtg ccatgatctg
    11161 gacatcaaac atgtccagcc agaacattaa cgccgctccg cacaacaaca gtgcccaggc
    11221 aataggcaaa ccgatagcaa taccacccaa cagacagccc agaaaaatca gcacagccat
    11281 gattaagctc cttgcggtgg cgacgttaga gaattgctac gcgtgatgag ttgatataaa
    11341 tgacgcagtt caaagaatgc gataacgagg ctggtgggca gacaagcggc atacatcagg
    11401 ccgatgggca aaccgaggat cggtgaataa tcgctccagt cctgaattgt ttttagcgtt
    11461 gcgccccagg ccagtgcgcc acaaataaat aagattaagg aatgggtaac cagagcgact
    11521 cgtcgctgcc atgcggggga gagtttctcc accagaaagg tgacctgaac gtgggcgtta
    11581 tccataaaag ccacaatcgc gccaataaat gttaaccaga caaataaata acgtgacaat
//
LOCUS       DAZUCC010000158        11495 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZUCC010000158 DAZUCC010000000
VERSION     DAZUCC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.159"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..943)
                     /gene="pyrD"
                     /locus_tag="ACHY09_003651"
     CDS             complement(<1..943)
                     /gene="pyrD"
                     /locus_tag="ACHY09_003651"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFS3886568.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASL"
     gene            1180..1755
                     /gene="ssuE"
                     /locus_tag="ACHY09_003652"
     CDS             1180..1755
                     /gene="ssuE"
                     /locus_tag="ACHY09_003652"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS3886569.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            1748..2707
                     /gene="ssuA"
                     /locus_tag="ACHY09_003653"
     CDS             1748..2707
                     /gene="ssuA"
                     /locus_tag="ACHY09_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFS3886570.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2704..3849
                     /gene="ssuD"
                     /locus_tag="ACHY09_003654"
     CDS             2704..3849
                     /gene="ssuD"
                     /locus_tag="ACHY09_003654"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFS3886571.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TDVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            3861..4652
                     /gene="ssuC"
                     /locus_tag="ACHY09_003655"
     CDS             3861..4652
                     /gene="ssuC"
                     /locus_tag="ACHY09_003655"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFS3886572.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            4649..5416
                     /gene="ssuB"
                     /locus_tag="ACHY09_003656"
     CDS             4649..5416
                     /gene="ssuB"
                     /locus_tag="ACHY09_003656"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFS3886573.1"
                     /translation="MNTARLNQGTPLLLNAVSKQYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(5622..8234)
                     /gene="pepN"
                     /locus_tag="ACHY09_003657"
     CDS             complement(5622..8234)
                     /gene="pepN"
                     /locus_tag="ACHY09_003657"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFS3886574.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVRWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQLLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            8500..9702
                     /gene="pncB"
                     /locus_tag="ACHY09_003658"
     CDS             8500..9702
                     /gene="pncB"
                     /locus_tag="ACHY09_003658"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS3886575.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQYLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9871..11271
                     /gene="asnS"
                     /locus_tag="ACHY09_003659"
     CDS             9871..11271
                     /gene="asnS"
                     /locus_tag="ACHY09_003659"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS3886576.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2626 a         2980 c         3039 g         2850 t
ORIGIN      
        1 ccagtgaggc ccccgcagca atcttttcac gcgcagcgat aaccgaatca atgccgccaa
       61 caccgatgat cggtaagcga ccgtttaatt cctgtgacaa gcggcgaata atttctgtgc
      121 tttttaactg aagcggacga ccacttaagc caccggtttg atcacaattt ttcattccct
      181 gaaccagaga acgatcgagc gtggtattgg ttgcaatcac gccatcaata ttatggcgaa
      241 ctaaactatc ggcaacctgg atcaattctt cttcagaaag atccggcgcg atcttcactg
      301 cgatcggcac atatttatgg tgcatcgctt gcaaatcatt ttgcttattt ttaatcgcgg
      361 ttaagagatc atccagcgct tcgccatatt gcagtgtgcg taatcctggg gtatttggcg
      421 atgaaatatt gatggcgata tatcccgcat aggcatagat tttttccata caaatcagat
      481 agtcatcttt accctgctcc accggcgtat ctttattttt gccgatgtta atacccagaa
      541 cgccgtcata atgggctttt tttacgttct ctacgaggtt atcaacgcca agattattaa
      601 agcccatacg gttgatcaaa ccttcggcat ctaccagacg aaagagacgc ggcttgtcat
      661 tacctggctg gggacgtggc gtgacggtac cgatctcgat cgatccaaat cccatcgcgc
      721 ctaacgcgtc aatgcactcc ccgtctttat caagaccggc tgccagacca agcggatttt
      781 taaacgtcag gcccatgcag ttaacaggtt tcgcaggcac tttctgccgc accagtgctt
      841 caaacggcgt tccggtaata cggcgtaatt gctgaaaagt aaactcatga gcgcgctctg
      901 gatcgagctg gaaaagggct ttacgaacga aggggtagta catgaactct cctggattcc
      961 cggtgtgcaa accgggggcg tattatgtgc gagttcggac caaaagggaa ttgacctgcg
     1021 gcaataaata gcaaacgttt tcttattgcg ctttttttat gctttttttg gcttttcttc
     1081 ctcagataaa tcatttcgac tcgcggacgc cctctgtcag actgaccgaa ttgttatcaa
     1141 tgttaacaaa acaagaacaa ttggttataa ggagagagta tgcgtgtcat caccctggcg
     1201 ggtagtcctc gctttccttc tcgctccagc tccttgctgg aatatgcgcg ggaaaaacta
     1261 aatggcctgg atgtagaggt ttatcactgg aatttgcaaa acttcgtccc ggaagatctg
     1321 ctttatgctc gtttcgatag tccggcactc aagaccttca ccgaacagct gcaacaggcc
     1381 gatgggctga ttgtcgccac gcctgtgtat aaagccgcct attccggcgc gctgaaaacc
     1441 ctgctcgacc tgctgccaga acgtgcgttg caaggcaaag tggtgctgcc gctggcgaca
     1501 ggcggcaccg tggcccatct gctggcggtc gattatgccc ttaagccagt tttaagtacg
     1561 ctgaaagccc aggagatcct gcacggcgtg tttgccgatg actcgcaagt aattgattac
     1621 caacacaaac cccagttcac gccaaatctg caaactcgtc ttgataccgc gctagaaact
     1681 ttctggcagg cgttgcaccg ccgcgatgtt caggttcctg accttctgtc tctgcgaggt
     1741 aatgcccatg cgtaagatca ttaaactggc gctggcgggt ttacttagcg tctctacact
     1801 tgctgttgcc gcggagtctt cgccagaggc gttacgtata ggctatcaaa aaggcagtat
     1861 tggtatggtg ctggcaaaaa gccaccagtt actggaaaaa cgctatccgc aaacaaaaat
     1921 ctcctgggtg gagttccccg ctggcccaca gatgctggag gcgttaaacg ttggcagtat
     1981 cgatctcggc agtaccgggg atattccgcc aatctttgcc caggctgccg gggctgattt
     2041 ggtatacgtg ggcgtcgagc caccgaaacc caaagccgaa gtgattctgg tggcagaaaa
     2101 cagcccgatc aaaaccgtag ccgatcttaa aggtcacaaa gttgcctttc agaaaggttc
     2161 cagttcgcac aatcttttac tgcgagccct tcgccaggcc gggcttaaat tcacggatat
     2221 tcagcccact tacctgacgc ctgccgatgc ccgcgccgcg ttccagcaag gtaacgttga
     2281 cgcctgggct atctgggatc cctactactc cgctgcatta ttacagggcg gcgtgcgggt
     2341 gctgaaagac ggcaccgatc tcaatcaaac tggatcgttt tatctggcag ctcgtccgta
     2401 tgcagaaaaa aacggcgctt ttattcaggg tgtgctgaca acctttagtg aggccgatgc
     2461 gttaacccgc agccagcgcg aacaaagcat cgctttactg gcaaaaacga tgggcttacc
     2521 ggcaccggtt attgcctcat atctggatca tcgtcctccc accaccatca aaccgttgag
     2581 cgctgaagtt gccgccttac agcagcaaac ggcagatctg ttttatgaaa accgtctggt
     2641 gccgaaaaaa gtcgatattc gccagcgcat ctggcagccc actcaactgg aaggaaaaca
     2701 attatgagtc tgaatatgtt ctggttttta ccgacccatg gtgacgggca ctatctggga
     2761 acggaagaag gttcacgtcc ggttgatcac ggttatctgc aacaaattgc gcaagcggcg
     2821 gatcgtctgg gttataccga tgtgctgatc ccaacaggac gatcgtgtga agatgcgtgg
     2881 ctggttgccg cgtcgatgat cccggtgacg caacggctga agtttcttgt cgccctgcgt
     2941 cccagcgtaa cctcacctac cgttgccgcc cgccaggccg ccacgcttga ccgtctctcg
     3001 aatggtcgtg cgttgtttaa tctggtcaca ggcagcgatc cacaagagct ggcaggcgac
     3061 ggcgtgttcc ttgatcatag cgagcgctac gaagcctcgg cggaatttac ccaggtctgg
     3121 cggcgcttat tgctggggga aaccgtcaat ttcaacggta aacatattca tgtgcgcgga
     3181 gccaaactgc tcttcccgcc gattcaacag ccgtatcctc cgctttactt tggcggatcg
     3241 tcagatgtcg cccaggagct ggcggcagaa caagttgatc tctacctcac ctggggcgaa
     3301 ccgccggaac tggttaaaga gaaaatcgaa caagtgcggg cgaaagctgc cgcgcatgga
     3361 cgcaaaattc gtttcggtat tcgtctgcat gtgattgttc gtgaaaccaa cgacgaagcg
     3421 tggcaggccg ccgagcggtt aatctcgcat cttgatgatg agactatcgc caaagcacaa
     3481 gccgcattcg cccggacgga ttccgtagga caacagcgaa tggcggcgct acataacggc
     3541 aagcgcgaca atctggagat cagccccaat ttatgggcgg gcgttggctt agtgcgcggc
     3601 ggtgccggga cggcactggt gggcgatggt ccgacggtcg ctgcgcgaat caacgaatac
     3661 gccgcgcttg gcatcgacag ctttgtgctt tcgggctacc cgcatctgga agaagcgtat
     3721 cgggttggcg agttgctgtt cccgcatctg gatgtcgcca tcccggaaat tccccagccg
     3781 cagccgctga atccgcaagg cgaagcggtg gcaaatgatt ttatcccccg taacgtcgcg
     3841 caaagctaag gaatacgata atggcaacgc cagtgaaaaa gtggttattg cgcgttgccc
     3901 cctggttttt accggtgggc atcgtggcgg tgtggcaact ggcctcctca gttggctggc
     3961 tgtcaacgcg tattttgccc tcaccggaag gagtggtgat ggcgttctgg acgctctccg
     4021 ccagcggcga attgtggcag catctggcga tcagctcctg gcgggcgctc attggctttt
     4081 caattggcgg atcgctgggg ttgattttgg gactgattag cgggctatct cgctggggag
     4141 aacgactgct ggatacctcc attcagatgt tgcgtaacgt gccgcatctg gcgctgatcc
     4201 cgctggtgat tttgtggttt ggcattgatg aatccgcaaa aatctttctg gtggcgctcg
     4261 gcacgctatt ccccatttat atcaacacct ggcacggcat ccgtaatatc gatcgcggtc
     4321 tggtggaaat ggcgcgtagc tatggattat ccggcatacc gctgtttatc catgtgatcc
     4381 tgcctggcgc cctgccctca attatggtcg gcgtgcgttt tgctttgggc ctgatgtggc
     4441 tgacgctgat cgttgccgaa actatttctg ccaattccgg cattggttat ctggcgatga
     4501 atgcgcggga gtttctgcaa acggacgtgg tggtagtcgc tattattctt tacgccctgc
     4561 tcggcaaact tgctgacgtc agcgcgcagc tgttagagcg tctctggttg cgctggaacc
     4621 cggcttatca tttgaaggag gccacggtat gaatactgct cgtctgaacc agggcacacc
     4681 attgttgctc aatgcagtaa gcaaacaata cgcggaaaat atcgtcctga accaactgga
     4741 tttacatatt ccggcaggtc agtttgtggc ggtggtgggc cgcagcggtg gtggcaaaag
     4801 taccctgctg cgcctgctgg caggtctgga aacgccaacc gcaggcgacg tgttagcggg
     4861 caccacaccg ctggctgaga ttcaggaaga cacgcgaatg atgtttcagg atgcgcgtct
     4921 gctaccgtgg aaatcggtga ttgataacgt tgggttaggc cttaaaggcc agtggcgcga
     4981 tgccgcgcgt cgggcgttgg ctgcagtagg actggagaac cgcgcagggg aatggcctgc
     5041 cgcactttct ggcgggcaga aacagcgtgt ggcgctggca agagcgttaa ttcatcgacc
     5101 gggtttatta ttgcttgatg aaccgctcgg ggcgctggac gccctgactc ggctcgagat
     5161 gcaggatttg attgtgtcac tttggcagga gcacggcttt accgtactgt tggtgacgca
     5221 tgatgtgagc gaagcggtag caatggctga ccgggtattg ttaattgaag agggaaaaat
     5281 tggtctggat ttgacggtgg atatcccccg tccgcgccgg ttagggtcgg tgcgattagc
     5341 ggagctggaa gcggaagttt tgcaacgggt gatgcagcgt gggcactctg aacaaccgat
     5401 tcggcgtcat ggttaatatg ccggatgtgg cacacaaaac cagcagtttc aacggattgt
     5461 aggcctgata agacgcgtca agcgtcgcat caggcatttg ggcacggctg ccggatgcgg
     5521 cgtgaacgcc ttatccagcc tacaaaacca gcattttcaa cggacttgta ggcctgataa
     5581 gacgcgtcaa gcgtcgcatc cggcattttg gctcaaaagt atcaagccag tgctttagtt
     5641 atcttctcgt acagatcgcc agagagattt tccagccctt tcaactgctc cagcgccgcg
     5701 cgcattttct cctgacgttt ggcatcgtaa cgtttcaggc gaatcagcgg ctcaatcagg
     5761 cgtgaagcca cctgcgggtt acggctgttg aggtcggtaa gcatttccac caggaactgg
     5821 taaccgctgc catcttcggc atggaacgct gccgggttgc tgcccgcaaa cgcaccaatc
     5881 aacgaacgaa tacggttcgg gttgctcatg gtaaatgagc gatgctgcaa caggccacgc
     5941 accgtctcca gcacattcgc cgccgggctg gtggcttgca ggataaacca tttatccatc
     6001 accagaccat cctgatgcca cttgtcgtcg tactcctgca tcagcgcgtc acggcaaggc
     6061 agctgtgcag caaccgccgc agaaagcgct gccagcgcat cggtcatatt gtttgcttca
     6121 tggtattgct tgctcaccag cacgtcagcc agatgcgttt caccaaaagc aaggaagcgc
     6181 aggcaggcat tacgcagagt gcgttttgca atatcttcat gctcaacacg gtactcgctc
     6241 tggtaattcg cgttgtaaat agccagtagc tcatccgcca gttcagtcgc cagagtacga
     6301 gtgagtgctt cgcgtacttc ggcaatagca atcgggtcga tgatatcgaa cagttcagcc
     6361 atttcattga cagaaggcag cgtcaggatt tctgccgcca gcgctggatc aatcttctca
     6421 tcgagcagca ccgcgcggaa agcgtcagcc acatgcaccg gcagagacag cagctgccct
     6481 tgctgatgac gggcgacgtt tagcttgatg taggttgcca gcaggctttg cgccgcatcc
     6541 caacgggaga aatcattacg cgcatgacgc atcaggaagg tcagttgctg atcgctccac
     6601 ttatattcca gtttcactgg cgcagagaat tcgcacagca gcgcaggcac cggctggaag
     6661 tagacattat caaaaacaaa ggtctgttca gcctgggtga cgttcagcac cgaattcacc
     6721 ggatgaccgc ctttctgcaa cgggatcact ttgccttcgt tatcatacag ttcgatggca
     6781 aacggaatat gcagcggctg tttttctgcc tgatccggcg tagctggcgt gcgctggctg
     6841 atggtcaggg tgtactgctc agtttccgga ttgtagtcgt ctttgacagt cacaaccggc
     6901 gtgccggatt ggctgtacca acggcggaaa tgggaaagat cgacattcga cgcatcttcc
     6961 atcgcctgca caaagtcgtc acaggtcgct gcgctaccat catgacgctc gaaatagagc
     7021 tgcatccctt tctggaagtt ttcttcgcca agcagagtgt ggatcatacg aatcacttcc
     7081 gcgcccttct cgtaaacggt cagggtgtag aagttgttca tttcaatgac catatccggg
     7141 cgaattgggt gcgccatcgg gctggcgtct tctgcaaact gcaatccgcg catggtgcgc
     7201 acattattga tacggtttac cgcgcgggaa ccaaggtcag agctgaattc ctgatcgcgg
     7261 aagacggtta aaccttcttt cagactgagc tggaaccagt cgcgacaggt gacacggttg
     7321 ccggtccagt tatggaaata ttcatggccg ataacgcgtt caatatcgag gtaatctttg
     7381 tcggtggcgg tgtcggtgcg ggccagcaca tatttggagt taaagatatt cagcccctta
     7441 ttctccattg cgcccatatt gaagaaatcc accgcgacga tcatatagat gtcgaggtca
     7501 tactccaggc cgaagcgttc ttcatcccat ttcatggagt ttttcagcga ggtcattgcc
     7561 cacggcgcac gatcaaggtt gccacgatcg acatacagct ccagcgctac ttcgcgacca
     7621 gaacgcgtgg tgaaagtgtc gcgcagcaca tcaaagtcgc ctgccaccag cgcaaacagg
     7681 tagcacggtt tcgggaacgg gtcctgccac cgtacccaat gacgtccatt ttccagttca
     7741 ccttgcgcaa cgcggttacc gttggaaagc aggaagggat atttggtttt atcggcaata
     7801 attttggtgg taaaacgcgc cagcacatcc gggcggtcga gataatacgt aatatggcgg
     7861 aaaccttcgg cttcacactg ggtgcaaagc gcatcgcctg actgataaag cccttccagc
     7921 gcagtattcg ccgccgggct aatttcatta acgatcttaa gcgtaaaacg ctccggcaaa
     7981 ttactgatga ccagtgcgcc ctcttcttct ttccaggcgg tccacggctc atcattaata
     8041 taaacagaaa ccagtttgag gtcttcgccg ttgagacgaa gcggaacatc tgatgcacca
     8101 tgacggacag cctggctgac cgcggtaacg accgtctttt gcgcgtccag gtcaaaggtc
     8161 aagtcaatat cagtaatctg gtaatccggc gcacgataat cgtgacggta tttggcttgt
     8221 ggctgttgag tcataaataa cctttagcat cttttataga gtctggtgtt cagtctattc
     8281 ctgttgcgta aatcgcgcta tgcagaatct tcatcttttc aggtacaaac gcctttattg
     8341 ctacattttt ataacataca ccgcgtaatg ccatcgacca gaaaggtggc atatggtgtg
     8401 atcggggttc aataaattgc gaaacaaggt atactccagc agttcctgaa gatgtttatt
     8461 gtactaaacg ctcctgtacg aggacgctac tgcgcaccta tgacacaatt cgcttctcct
     8521 gttctgcact cgttgctgga tacagatgct tataagttgc atatgcagca agccgtgttt
     8581 catcactatt acgatgtgca tgtcgcggcg gagtttcgtt gccgaggtga cgatctgctg
     8641 ggtatttatg ccgatgctat tcgtgaacag gttcaggcga tgcagtacct gcgcctgcag
     8701 gatgatgaat atcagtggct ctctgccctg cctttcttta aggccgacta tcttaactgg
     8761 ctacgcgagt tccgctttaa cccggaacaa gtcaccgtgt ccaacgataa tggcaagctg
     8821 gatattcgtt taagcggccc gtggcgtgaa gtcatcctct gggaagttcc tttgctggcg
     8881 gttatcagtg aaatggtaca tcgctatcgc tcaccgcagg ccgacgttgc gcaagccctc
     8941 gacacgctgg aaagcaaatt agccgacttc tcggcattaa ccgccggtct tgatatgtcg
     9001 cgcttccatc tgatggattt tggcacccgc cgacgttttt ctcgtgaagt acaagaaacc
     9061 atcgtgaaac gtctgcaaca ggaatcctgg ttcgtgggca ccagcaacta cgatctggcg
     9121 cgtcggcttt ccctcacgcc gatgggaaca caggcacacg aatggttcca ggcgcatcag
     9181 caaatcagcc cggatctagc caacagccag cgagctgcac ttgctgcctg gctggaagag
     9241 tatcccgacc aacttggcat tgcgttaacc gactgcatca ctatggatgc tttcctgcga
     9301 gatttcggtg tcgagttcgc cagtcgctat cagggcctgc gccatgactc tggtgacccg
     9361 gttgaatggg gtgaaaaagc cattgcacat tatgaaaagc tgggaattga tccacagagt
     9421 aaaacgctgg ttttctctga caatctggat ttacgcaaag cggttgagct ataccgccac
     9481 ttctcttccc gcgtgcaatt aagttttggt attgggactc gcctgacctg cgatatcccc
     9541 caggtaaaac ccctgaatat tgtcattaag ttggtagagt gtaacggtaa accagtggcg
     9601 aaactttctg acagcccagg caaaactatc tgccacgata aagcgtttgt tcgggcgcta
     9661 cgcaaagcgt tcgaccttcc gcatattaaa aaagccagtt aatatcatca gggagctaat
     9721 cggctccctt tttttacctt taattccgaa atctttcgct gcatttgcga attctgcttg
     9781 tctgattgca gatgccaggt aacataggta tccccccatt tacgggtgga caagtgttta
     9841 ttttttccga ctattaacag agagaatatt atgagcgttg tgcctgtagc cgacgtactc
     9901 cagggccgcg tagccgttga cagcgaagtc accgtgcgcg gatgggtacg tacccgccga
     9961 gattcaaaag ctggcatctc cttcctcgcc gtttatgacg gttcctgctt tgatcctgta
    10021 caggctgtca tcaataattc tctgcccaat tacaatgaag acgtcttgcg tctgaccacc
    10081 ggctgctcgg tcattgtgac gggtaaagtc gtggcgtcgc cgggccaggg gcaacaattt
    10141 gaaattcagg ccagcaaggt tgaagttgct ggttgggttg aagatccaga cacttacccg
    10201 atggcggcaa aacgccacag cattgagtat ctgcgtgaag tcgctcacct gcgtccgcgc
    10261 acaaacctga ttggtgccgt cgcgcgcgtt cgccatacgc tggcgcaggc gctgcatcgc
    10321 ttctttaacg agcagggatt tttctgggtt tcaacgccac tgattaccgc atctgatacc
    10381 gaaggtgcag gcgaaatgtt ccgtgtttct acgctggatc tggaaaatct gccgcgtaac
    10441 gatcagggca aagtggattt cgacaaagac ttctttggta aagagtcttt cctgaccgta
    10501 tctggccagt tgaacggcga aacctacgct tgcgcattgt ccaaaattta taccttcggc
    10561 ccgactttcc gtgctgaaaa ctccaacacc agccgtcacc tggcggaatt ctggatgctg
    10621 gagccggaag tggcgtttgc taacctgaac gatatcgcgg gtctggctga agccatgctg
    10681 aaatatgtct tcaaagcggt tctcgaagaa cgcgctgacg acatgaaatt cttcgctgaa
    10741 cgcgtagata aagatgccgt ttcacgtctg gaacgcttca ttgaggccga tttcgcgcag
    10801 gtggattaca ccgacgcagt gaccattctc gaaaactgcg gcaggaagtt tgaaaacccg
    10861 gtttactggg gcgtcgatct ctcttctgag catgagcgtt atctggcgga agaacacttt
    10921 aaagcaccgg tagtggttaa aaactatccg aaagatatta aagcgttcta tatgcgcctt
    10981 aacgaagacg gtaaaaccgt tgcggctatg gacgttctgg ctccgggcat cggtgagatc
    11041 attggtggct cccagcgtga agaacgtctg gacgtgctgg acgagcgtat gctggaaatg
    11101 ggcctgaata aagaagatta ctggtggtat cgcgatctgc gtcgctacgg tactgttccg
    11161 cattcaggtt tcggtcttgg ttttgaacgc ctgattgctt acgtaactgg tgtgcaaaac
    11221 gtgcgtgatg tgattccgtt cccacgtact ccgcgtaacg ccagcttcta atttagcgtc
    11281 ttcaagagcc agcacccgct ggctcttttt ttatcctttc tgtcaagtta tctgtttgtt
    11341 aagtcaagca atctatttgc aaccccgcca taacgatcat cctgttacgg aatattacat
    11401 tgcaacattt acgcacaaaa aataacccgc attcttattg cggattagtt ttttcttagc
    11461 tgatagcaca attttcatac tattttttgg cattc
//
LOCUS       DAZUCC010000159        11420 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZUCC010000159 DAZUCC010000000
VERSION     DAZUCC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.160"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(137..943)
                     /gene="trpA"
                     /locus_tag="ACHY09_003660"
     CDS             complement(137..943)
                     /gene="trpA"
                     /locus_tag="ACHY09_003660"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFS3886577.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNLAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(943..2136)
                     /gene="trpB"
                     /locus_tag="ACHY09_003661"
     CDS             complement(943..2136)
                     /gene="trpB"
                     /locus_tag="ACHY09_003661"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFS3886578.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2148..3509)
                     /gene="trpCF"
                     /locus_tag="ACHY09_003662"
     CDS             complement(2148..3509)
                     /gene="trpCF"
                     /locus_tag="ACHY09_003662"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFS3886579.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPAHIATVYRNYASAISVLTDEKYFQGSFDFLS
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRHLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3510..5105)
                     /gene="trpD"
                     /locus_tag="ACHY09_003663"
     CDS             complement(3510..5105)
                     /gene="trpD"
                     /locus_tag="ACHY09_003663"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFS3886580.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHVPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDSQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5105..6667)
                     /gene="trpE"
                     /locus_tag="ACHY09_003664"
     CDS             complement(5105..6667)
                     /gene="trpE"
                     /locus_tag="ACHY09_003664"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFS3886581.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCEDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQSLLNVPNEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6689..6782)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6759..6803)
                     /gene="trpL"
                     /locus_tag="ACHY09_003665"
     CDS             complement(6759..6803)
                     /gene="trpL"
                     /locus_tag="ACHY09_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFS3886582.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6941..7822
                     /gene="rnm"
                     /locus_tag="ACHY09_003666"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6941..7822
                     /gene="rnm"
                     /locus_tag="ACHY09_003666"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFS3886583.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HYGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7819..8439
                     /gene="yciO"
                     /locus_tag="ACHY09_003667"
     CDS             7819..8439
                     /gene="yciO"
                     /locus_tag="ACHY09_003667"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS3886584.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8467..10356
                     /gene="yciQ"
                     /locus_tag="ACHY09_003668"
     CDS             8467..10356
                     /gene="yciQ"
                     /locus_tag="ACHY09_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFS3886585.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYPKNTEIPFYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVIRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAILREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKEKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWEGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEIARNGVLFSLQTHLSCGAYTSLLGRSYSGGGSGAGGG
                     GGGGW"
     gene            10569..>11420
                     /gene="rluB"
                     /locus_tag="ACHY09_003669"
     CDS             10569..>11420
                     /gene="rluB"
                     /locus_tag="ACHY09_003669"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFS3886586.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHC
                     QVSGGRRS"
BASE COUNT         2687 a         2910 c         3045 g         2778 t
ORIGIN      
        1 gcggaaaaaa gacgtaagtt gacgttcgac aggggcaagc gaaacggtaa aaagataaat
       61 aataaatgaa tttaggattt ttccagcttc gttaaagaaa gttaaaatgc cgccagcgga
      121 actggcggct gtgggattaa ctgcgcgtcg ccgctttcat cggttgtaca aaagctttca
      181 gtgccgccag cattttctct ggctcattaa tatgttgctc gatgatttta acgatggccg
      241 aaccagaaat cgcgcccgca gctcctgcat caatcgctgc ttttacctga tccggggcgg
      301 aaataccaaa gccctgcaat ggaggcgcag cattgtattc tttcagcttc gtgaccagat
      361 gattgagggg taacgcggcg cggttttctg cgccggtcac gcccgcacgt gacaataaat
      421 aggtgtatcc acgaccgtag gaggctatct ggcgcagcaa atcatcgtcg gcattcggcg
      481 ggcagataaa gataggtgca agattatgac gcaacgcggc ctggcggaag ggagcggact
      541 cttccactgg cacatcggca accagcaccg aatcgacgcc gactttctcg cactgggcat
      601 aaaactcatc aatgcctttg ttaaacacca ggttggcata catcagcagg ccaatgggaa
      661 tggtcgggtg tttctggcga atcagtgcca gcatttcaaa acattgtgcc ggagtcacac
      721 ctgccgcaaa ggcgcgcaga gtggcgtttt gaatcgtcgg gccatccgcc agtgggtcgg
      781 agaaggggat acctaactcc agcgcgtcag caccggcttc aattagcgtg tcgataattt
      841 tcagcgactg ctcaatgccc ggatcaccga gcgtgacgaa aggaacgaat gcgccttctt
      901 tgcgctcctt caactgggca aacagagatt cgtagcgttc catcagattt cccctcgtgc
      961 tttcaaaata tcgtgaacgg tgaagatgtc tttatcgccg cgaccggaaa ggttaaccac
     1021 cagtagctgc tctttttccg ggttttcgcg catcattttc agcgcatggg ccagggcgtg
     1081 tgaggactcc agcgccggga taatcccttc gtgcaggcag agcgttttga aggcttccag
     1141 tgcttcatca tcggtaattg acacgtaatc agcgcgtcca gtgctgttaa gatacgcgtg
     1201 ttgtgggccg acagacggga aatccagtcc ggcggagatg gagtaagatt cttcaatctg
     1261 cccgtcttcg gtttgcatca tcggcgcttt cataccgaaa tagatgccca cgcgaccgtg
     1321 ttttaacggt gcgccgtgct cgccagtttc gataccgtga ccacctggct ccacaccaat
     1381 caggccgacg ttggtttcat tgatgaaatc agcaaacatg ccgatggcat tcgaaccgcc
     1441 gccaacacag gcgataacag catccggcag gcgaccttct ctttccagaa tctgcgcttt
     1501 cgtttcttcg ccaatcatcc gctgaaactc acgcacaatg gtcgggtaag gatgcgggcc
     1561 agccgcggtg cccagcatat agtgcgcggt ttcataactg ccggaccagt cgcgtagcgc
     1621 ctcattacag gcatctttca gcgtcgcgga accgctatgc accgggatca cttccgcacc
     1681 cattaaacgc atacgaaaaa cgttaggcga ctggcgttca acgtctttgg cacccatata
     1741 aatgcgacat ttcaggccga gcagggcgct ggcaagggcc gacgccacgc catgctgacc
     1801 ggctccggtt tcggcgatga tttccgtttt acccatccgc ttcgccagca acgcctggcc
     1861 gagcacttgg ttggttttat gcgcgccgcc gtgcagcaaa tcttcgcgct tgagatacag
     1921 cgtggtgtta gtgccagccg taatgttctg gcatttggtg agtgcggttg gacgcccggc
     1981 atagtttttc agcaggtcgt tgaactgagc ctgaaattca ggatcttttt gcgcgctaac
     2041 aaaagcttct tccagctggc gcagagcagg catcaggatt tgtggcacgt acatgccgcc
     2101 aaactcacca aaatacgggt taagtaatgt tgtcattgtt cctttcctta atatgcgcgc
     2161 agcgtctgga aaaccgaggc caaaagacgt gcgtctttga tgcccggttg cgactctaca
     2221 gcagaattaa aatcaagtcc ggcgcagccg gtttgtgccg cttccacgca gttatctgcg
     2281 cctaagcccc ccgccagcag aacgttgcca agcgattgac cgtttaacag tgaccagtcg
     2341 aaacgttgac cgcttccacc ctggccgttg tctaaaacat atttatcaac gtgctgaagc
     2401 tcgcgggcgg gcagggtttc accgacgctt aatgctttcc agatggcaac gtgtgctggc
     2461 agagcttcac gcagcgtatc gatatacagc tgatcttcat taccatgcag ttgcactgcc
     2521 gccagcgata acaccttagc tttgtccacc acatcggcaa tatcgtgatt gcggaacacg
     2581 ccaacatact gcaacggtgc cgcagccatc acttcctgcg cctgttcaac gttgacgcaa
     2641 cgcggtgatg tcgcaacaaa aatcaaccca ccgtaaatcg cgcccgcgtc ataagctgct
     2701 ttagcatctt gcccacgcgt caggccacat actttattct cacccagcaa tacccggcgc
     2761 acggcggcgt tcaaatcgtc atgggccatc aacgccgaac caatcagaaa accgttagcg
     2821 aagtggctta actcgcgcac ctgagcgtaa gtattgatgc cggattcgct gattaccgtc
     2881 acgttgtgcc ccagtttcgg cgcaagctcg cgggtacggt tgagatcaat cgacaaatca
     2941 cgcagatcgc ggttgttgat gccaacgacc tttgccccca atgcaatggc gcgctccagt
     3001 tcctcttcat tactgacttc ggtcagcaca cccatctcca gactgtgggc gacggcggca
     3061 aggtggcgat attgttcgtc atccagtact gaaagcatta ataagcaggc atcggcctgg
     3121 taatagcgcg ccagatagat ctggtaaggg tcgataataa agtctttaca taaaatcggc
     3181 tgcggggcga tttggctgac gatggagagg aaatcaaagc tcccctgaaa atatttctca
     3241 tcagtcagca ccgaaattgc cgaagcgtaa tttctgtaga cggttgcaat gtgtgccgga
     3301 tcaaaatcat cgcggatcac gccttttgac ggtgacgctt ttttacactc cagaataaac
     3361 gccgtgcgtg caccctgtag cgcatcataa aaatgtcgcg tgctcggctg aacctcattc
     3421 tgaaaactgg ccagcggttg ctgctgtttg cgggcttcta cccaaatcgc cttgtctgcg
     3481 acgattttcg ctaaaacggt ttgcatcatt taccctcgtg ccgccagtgc ggtgactctg
     3541 tcgtaagcgg aaccactgcg cagtacctca agaacggttt gcgcattggc ttgcagatct
     3601 tcatggccat gcaggcgcat taacatggcg acgttcgccg cgacggctgc ttcatgggcg
     3661 gcgtcgcctt taccttgtaa caagcgtgtt aaaatgtcac ggttttcttc cggtgttccg
     3721 cctgccagtt gctcctggtg gtagggtgtc aggccaaaat cttctgcagt tagctgatag
     3781 ctcttaattt caccgtcatg cagttcggca acgattgtcg gcgcgtgtaa tgaaacttca
     3841 tccatcccgc cgctgtgcac taccgcagcg cgttgatacc ccagcacgcg caaggtttcg
     3901 gcaatcggca gcaccagttc cggactataa acaccaatta gcgccagcgg cggatgcgcc
     3961 gggttaatca atggccccaa cacgttgaac agggtgcggg ttttcagttg ctggcgaacc
     4021 ggcatcgcgt ggcggaatcc ggtgtgatat ttcggcgcaa agaggaaaca gacgcctaac
     4081 tcatccagcg cctggcgcga tttatcggcg ttcatatcaa gattaatacc gaacgccgcc
     4141 agcagatcgg acgagccgga tttactggag acgctgcggt taccgtgttt cgccactttc
     4201 agcccacagg ccgcggcgac aaacgcactg gcggtagaaa tattgatgct gttgctgccg
     4261 tcaccgccag taccgacgat atcggcaaac agataatccg ggcgcgggaa cggcgcggcg
     4321 ttttccagta gcgcggttgc tgctccggcg atctcgttcg ggtgctcgcc gcgaattttc
     4381 atgctcacca gcgccgccgc cagttgttcc ggcttcagct cgccacgcac caccgctgaa
     4441 aacagctggt gactttcttg ttggctaagc gtctgtgcct gatacagttt ttccagaatc
     4501 ggttgcagcg tgttggttgg ctcaagtttc tgctgcgccc aggccagcgt ttgttccagc
     4561 aggcgagcgc cctgggtagt aagaatggat tccggatgga actggaaacc gcaaacgcga
     4621 tctgcatcgt gacgcaccgc catcaccatg ccattaaaat gggcgttgat ggttaaacct
     4681 gccgggatat tactgccaac cagcgagtga taacgcgcca ccggcaatgg gtttgttaat
     4741 ccggcaaaca tcgcctgact gtcatgttca atgctcgacg ctttaccgtg aagaatttcg
     4801 cccgcctgac cgacatagcc cccgtaagct tcgacaattg cctgatgtcc gaggcaaatg
     4861 ccaataattg gcagcttgcc acgcaagcgg gtgaggagtt ccggcataca accggcttcg
     4921 ctcggcacac cggggccagg agaaagcatc agcaccggat tgctcatcgt cgccaggcgt
     4981 tcaattaagg tctgcgccgg aacatggttg cggtaaatca ccacgttatg accattgctg
     5041 cgcaactgat ctgccaggtt gtacgtaaaa gagtcgatat tatcgagcag cagaatgtca
     5101 gccatcagaa cgtctcctgt gcatgatgcg cggtggcaat agcgcgcagt acagcgcggg
     5161 ctttattacg ggtttcgtcg gcttccgact gcggaacaga atcaaggact acgccagcac
     5221 cggcttgcac ggtggcgata ccgttttcca ccagcgccga gcggatcaca atgcaggtgt
     5281 cgagatcgcc atgcgcggta aaataaccta ccgcgccgcc gtagctgccg cgtcgacgac
     5341 cttctgcttc ggcaattaac tgcatagcgc gcactttcgg cgcaccgctt aatgtcccca
     5401 tattcataca ggcgcggtaa gcgtgcaggg cgtcgagatc gtggcgcagc tcaccgacca
     5461 cgcgggagac gaggtgcatc acataagaat aacggtcaac tttggtgaga tcggcgacgt
     5521 agcgactacc gggagtgcaa atacgcgcca ggtcattacg ggcgagatcc actagcatca
     5581 gatgttcaga aagctcttta tgatcggtac gcatttccag ttcgatgcgg ctgtcgaggt
     5641 ctctgtccag tgaaccatcg gcacgacgac cgcgtggacg tgttccggca atcgggtaga
     5701 tctcaatctg gcggctggtg gcgtcatact tgagcgaact ttccggcgac gcgccaaaca
     5761 gggtgaaatc attatcctgc ataaaaaaca tgtacgggct gggattactc tttttcagca
     5821 cgtaataggc cgccagcggt gacgggcagg gcagagagaa acggcgagat ggcaccacct
     5881 ggaaaatttc tccggcgcga atcgcttttt gcagcgaacg cactacgcca ccgaactctt
     5941 catcactctg gttacattca caacgcatat gcggcacgga aaccaccggc agcggcggcg
     6001 cggcttcggt cagttgctga cgtagttcgt tcaggcgagc agtgagacgt tgtttttctt
     6061 cttcattcgg agcaaacagg ctggcctgaa tacgggtgct ttttttctga tggtcaatca
     6121 ccatcagcgt ttcagcgaga taaaaacaga aatcaggaca gttattttcc gctgccagtt
     6181 gtggtaaatc ttcaaatccc gccacaaggt cataagagaa cagaccgccg aagaacattg
     6241 cttctcgttc ttcattcggt acattcaaca gactctgcaa taagcggaaa gcgtcaaaaa
     6301 ccgaaaggga gcataagcga gcgtcttcat ccagcagtgg actgacaggg gggaagcgca
     6361 gcacacggca gtttggtgat tgttcatttt ccacacccgc aggcagggcg ttatccagta
     6421 gtgccagcag ggcttcgcca ttgccggaaa gtgcctggat tgtgacagtg tcacctaaag
     6481 ctgtaatgcg cagcgcactg tctaccagca gcaggctttt taaatcatct ttgctgtcga
     6541 tatctgcgga ttccagtagc agcgttgctg gacgatcctc acacaactgg tgaaaaagcg
     6601 cggtgggatt gtcgcgataa gcgccttcgc aggttagcag ttcgagagtc ggtttttgtg
     6661 tttgcattgt tattctctaa ttttgttcaa aaaaaagccc gctcattagg cgggctgggt
     6721 atctgattgc tttacgcatg gtgaatacac tgcccgtttc aggaagtgcg ccaccaacct
     6781 ttcagtacga aaattgcttt cattgtcgat acccttttta cgtgaacttg cgtactagtt
     6841 aactagttcg atgattaatt gtcaacagct catttcagaa tatttgccag aaccgttatg
     6901 atgtcggcgc aaaaaacatt atccagaacg ggagtgcgcc ttgagcgaca cgaattacgc
     6961 agtgatttac gacctgcaca gccataccac agcttccgat ggctgcctga cgccagaagc
     7021 attggtgcac cgtgcagtcg agatgcgcgt cggcaccctg gcgatcaccg accatgacac
     7081 cacagctgca atctcaccag caagagagga aatttcacgt tcgggactgg cgctgaatct
     7141 tattcccggc gtggaaattt ccacggtctg ggaaaatcat gaaattcata ttgtcgggct
     7201 gaatattgat attactcatc cgctaatgtg tgagttcctc gcgcagcaga cagaacggcg
     7261 caatcagcgg gcgcagctga ttgccgaaag acttgaaaaa gcgcaaatac ctggggcact
     7321 ggaaggcgcg caacgactgg cgcagggcgg tgcggtgacg cgcggtcatt ttgctcgttt
     7381 ccttgtggag tgcggcaaag ccagttcaat ggcggatgtc tttaaaaagt atctggcgcg
     7441 cgggaaaacc ggatacgttc cgccacagtg gtgtacaata gaacaagcta ttgatgtcat
     7501 tcatcattct ggcggtaagg cggtgctagc tcatcctggg cggtacaatc ttagtgctaa
     7561 atggctgaaa agattggttg cgcattttgc cgaacactac ggcgacgcga tggaagtcgc
     7621 gcagtgccag caatcgccca atgaacgcac ccagctggcg acacttgcgc gtcagcatca
     7681 tttatgggca tcacaaggat ctgattttca tcagccatgc ccgtggatcg aactgggtcg
     7741 taaactctgg ctgcccgcag gcgttgaagg cgtctggcag ctatgggaac agccgcagaa
     7801 caccacagag agggaattat gagccagttt ttttatattc atcctgataa cccgcagcaa
     7861 cgcctgatca accaggcggt ggagatcgtg cgtaaaggcg gggtgattgt ttatccaact
     7921 gattccggct atgcgctcgg ctgtaaaatt gaagataaaa acgcgatgga gcgtatttgt
     7981 cgaattcgcc agctgccgga cggtcacaac tttaccctga tgtgtcgcga tctttctgag
     8041 ctctcgacct attcattcgt ggataacgtt gcgtttcgtt tgatgaaaaa caacacgccg
     8101 ggcaactaca ccttcatcct gaaagggacg aaggaggtgc cacgccgtct gttgcaggaa
     8161 aaacgcaaaa ccatcggtat gcgtgtgcct tctaacccga ttgcgcaggc gctgcttgaa
     8221 gcattgggcg aaccgatgct ttccacatca ctgatgctgc caggcagcga atttaccgaa
     8281 tccgatccgg aagaaattaa agaccgtctg gaaaaacagg tggacttgat tattcacggc
     8341 ggctatctcg ggcagaaacc aacaacggtt attgatctta ctgatgatac gccggtcgtg
     8401 gtgcgtgaag gcgtaggtga tgtgaagcct ttcttataac aaattgcgtt ccacggatgg
     8461 aagactatgg cagggatata tcgctgcatt ttgctgttga tagttgggct ttttttctct
     8521 tcgttgagtt atccgaaaaa cacggagatc cctttttatg aagaagggat ctcgctcttt
     8581 gatgttgaag ccactctgca accggatggg gtgctcgaca tcaaagaaaa tattcatttt
     8641 caggcgcgaa atcagcagat taagcacgga ttttatcgtg atttaccacg actctggatg
     8701 cagcctgatg gggacgctgc actgctgaac tatcatattg ttggcgtcat ccgtgatggt
     8761 attcctgaac cctggcatct tgactggcat atcgggttaa tgagtattgt cgtgggcgat
     8821 aaacaacgtt tcttgcctca aggcgactat cattatcaaa ttcattatca ggttaaaaat
     8881 gctatcctgc gtgaggggga ttctgatctg ctaatctgga acgtgaccgg taaccactgg
     8941 ccgtttgaaa tttataagac cctattttca ctcaagttgc ctgatattgc gggtaatcca
     9001 tttagcgaaa tcgatctctt tactggagaa gagggcgaca catatcgaaa tggccgcatc
     9061 cttgaggacg gaagaattga atctcgcgat ccgttttatc gtgaagattt cacggtcctc
     9121 taccgctggc ctcacgcatt acttggtaat gccccggctc cacaaacgac gaatattttc
     9181 agtcatcttc ttttaccctc aacgtcatcg ttgttaattt gctttccgag tctgttcctg
     9241 gcgtgtggat ggttatatct ctggaagcgc aggccgcaat ttacgccggt agatgtgatt
     9301 gaaaccgatg tcattccgcc agattacacg cccggcatgt tacgtcttga tgcgaaactg
     9361 gtttacgacg ataaaggttt ttgcgccgat atcgtaaatc tgattgtgaa agaaaaaatt
     9421 catctggaag atcattatga caagaaccag caaatcctga ttcgtgttaa tgaaggcgcg
     9481 accagaaata atgcggtatt actgcccgca gagcagttat tactggaagc gttatttcgt
     9541 aaaggcgata aggtcgttct tacggggaga cgcaacagag tcttacgcaa ggcattttta
     9601 cggatgcaga aattttatct gccgcgtaaa aagtcttcgt tttaccgacc tgatgcgttt
     9661 ttgcaatggg gcggaatggc aatattggcg gtcattctct acggtaacct gagtcccgta
     9721 ggctgggaag gaatgagtct ggtgggcgat atgtttatta tgatctgctg gcttcttcct
     9781 tttttatttt gttcccttga gcttttgttt gcccgcgatg atgacaagcc ttgcgttaat
     9841 cgtgtcatca tcactttgtt tttaccactg atttgttcag gtgtggcttt ttactctctc
     9901 tacatcaatg tcggagatgt attcttttac tggtatatgc cagcgggtta ttttagcgct
     9961 gttttcctga ccggttatct cactggcatg gggtatattt ttctgccaaa gtttacccaa
    10021 actgggcagc aacgttatgc ccacggtgaa gctatcgtta actatcttgc gcgtaaagag
    10081 gcagcaacac acagtgggcg gcgtaggaaa ggggaaacac ggaaactgga ttacgctttg
    10141 ttaggttggg ctatatcggc aaatttgggg agggaatggg cgttacgcat cgccccttcg
    10201 ctttcttcgg cgatttgcgc tccagagatt gcccgtaacg gcgttttatt ctcattacag
    10261 acgcacctga gttgcggggc ctataccagt ttgttggggc ggagctattc tggtggtggt
    10321 tctggcgctg gtggcggagg cggtggtggc tggtaatcaa gctgatgtta atcgaaacaa
    10381 gctttaacca taaagatagt gtagtatgtt gcgcctcaaa gccaggcaag ttaaaacgtc
    10441 gagtcgttta cttaaggcct gaagagttca aacagacttt actatcagtg agtcagatgt
    10501 aaattctgac tcactgttgc acaaaagcat atcagacgcc tgggaaggcg acaccaaaag
    10561 gaagctctat gagcgaaaag ctacagaaag tgctggcgcg tgccggccac ggttctcgcc
    10621 gtgaaatcga atctattatt gaagccggtc gtgtgagtgt tgatggcaaa attgctaaac
    10681 tcggcgatcg cgttgaaatt acccctggat tgaaaatccg tatcgatggt cacctgattt
    10741 cggtacgtga gtccgctgaa caaatttgtc gcgttctggc ctattacaaa ccggaaggtg
    10801 agttgtgtac ccgtaacgat ccggaagggc gtccaacggt gtttgaccgt ctgccaaagc
    10861 tgcgtggcgc acgctggatt gccgtaggtc gcctggacgt taatacctgt ggtctgctgc
    10921 tgttcactac cgacggtgaa ctggcaaacc gtttaatgca tccaagccgt gaagttgaac
    10981 gtgaatacgc cgtgcgcgta tttggtcagg ttgacgacgc gaaactgcgt gatttgagtc
    11041 gtggcgtgca gctggaagat ggtccggcag cctttaaaac catcaagttc agcggcggcg
    11101 aagggatcaa ccagtggtac aacgttactc tgaccgaagg ccgtaaccgt gaagttcgtc
    11161 gtctgtggga agcggttggt gtgcaggtaa gccgcctgat ccgtgttcgt tacggtgata
    11221 tcccactgcc gaaaggtctg ccacgcggtg gctggacgga gctggacctc gcccagacta
    11281 actatctgcg cgaactggtg gagctaccgc cagaaaccag ctctaaagtc gctgtagaaa
    11341 aagaccgtcg tcgtatgaag gcgaatcaga ttcgtcgtgc ggtgaaacgt cactgtcagg
    11401 tgagcggcgg tcgtcgttct
//
LOCUS       DAZUCC010000160        11417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZUCC010000160 DAZUCC010000000
VERSION     DAZUCC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.161"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..432
                     /locus_tag="ACHY09_003670"
     CDS             <1..432
                     /locus_tag="ACHY09_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFS3886587.1"
                     /translation="GGHPGLQNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVF
                     ASLNDEQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDS
                     KFRALAAELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            447..1304
                     /gene="menB"
                     /locus_tag="ACHY09_003671"
     CDS             447..1304
                     /gene="menB"
                     /locus_tag="ACHY09_003671"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFS3886588.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1304..2266
                     /gene="menC"
                     /locus_tag="ACHY09_003672"
     CDS             1304..2266
                     /gene="menC"
                     /locus_tag="ACHY09_003672"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFS3886589.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSGLLAWVNNWLAGDSELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSLLPLVDVDALERLL"
     gene            2263..3618
                     /gene="menE"
                     /locus_tag="ACHY09_003673"
     CDS             2263..3618
                     /gene="menE"
                     /locus_tag="ACHY09_003673"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFS3886590.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMVEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLDEWVKDKLARFQQPVRWLNLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3728..3994
                     /gene="pmrD"
                     /locus_tag="ACHY09_003674"
     CDS             3728..3994
                     /gene="pmrD"
                     /locus_tag="ACHY09_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFS3886591.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(3988..4374)
                     /gene="arnF"
                     /locus_tag="ACHY09_003675"
     CDS             complement(3988..4374)
                     /gene="arnF"
                     /locus_tag="ACHY09_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFS3886592.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4374..4709)
                     /gene="arnE"
                     /locus_tag="ACHY09_003676"
     CDS             complement(4374..4709)
                     /gene="arnE"
                     /locus_tag="ACHY09_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFS3886593.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4706..6358)
                     /gene="arnT"
                     /locus_tag="ACHY09_003677"
     CDS             complement(4706..6358)
                     /gene="arnT"
                     /locus_tag="ACHY09_003677"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFS3886594.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGAMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6358..7248)
                     /gene="arnD"
                     /locus_tag="ACHY09_003678"
     CDS             complement(6358..7248)
                     /gene="arnD"
                     /locus_tag="ACHY09_003678"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFS3886595.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGMLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7245..9227)
                     /gene="arnA"
                     /locus_tag="ACHY09_003679"
     CDS             complement(7245..9227)
                     /gene="arnA"
                     /locus_tag="ACHY09_003679"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFS3886596.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPTIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LTVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9227..10195)
                     /gene="arnC"
                     /locus_tag="ACHY09_003680"
     CDS             complement(9227..10195)
                     /gene="arnC"
                     /locus_tag="ACHY09_003680"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFS3886597.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10199..11338)
                     /gene="arnB"
                     /locus_tag="ACHY09_003681"
     CDS             complement(10199..11338)
                     /gene="arnB"
                     /locus_tag="ACHY09_003681"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFS3886598.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKSTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2776 a         2971 c         3069 g         2601 t
ORIGIN      
        1 ggcgggcatc ctgggttgca aaatgctgaa caacgcacgg aacgtcagcg ttccgatcgc
       61 caatgggcgc agcgttttcg cacagaaccg ttaacggcgg tatttgctga ctggtatcaa
      121 cagcctgttt ttgcctcact caatgacgaa caacgccggg agctggttgc gctgcgcagc
      181 aacaataatg gcgcaacact tgccgccatg ctggaggcga cttctctcgc cgtccagccc
      241 gatttacgtg ctaaccttag cgcccgcaca tttgcgtttt attatttatg tggtgaacgt
      301 gacagcaaat tccgcgccct ggcggcggaa ctggctgccg aatgccatgt cattcctcgc
      361 gccggacata acgcgcaccg ggaaaatccc gctggcgtaa tcgcaagtct ggcgcagatc
      421 ttgcgtttct gactaaagga cacaatatga tttatcctga tgaagcaatg ctttacgcac
      481 cggttgaatg gcacgactgc tccgaaggtt ttgaggacat tcgttatgaa aaatccaccg
      541 acggtatcgc aaaaatcacc attaatcgtc cgcaggtgcg caatgccttc cgtcctctga
      601 cggtaaaaga gatgatccag gcactggcag atgcgcgtta tgacgacaac atcggcgtga
      661 tcattctgac tggcgcaggc gataaagcgt tctgctccgg cggtgaccag aaagtgcgtg
      721 gtgattacgg cggctataag gatgattccg gcgtacatca cctgaacgtg ctggacttcc
      781 agcgtcagat ccgtacctgc ccgaaaccgg ttgtcgcgat ggtggctggc tactccattg
      841 gtggcggtca cgttctgcac atgatgtgcg acctgactat cgcggcagat aatgccatct
      901 tcggtcagac tggcccgaaa gtcggttcct tcgacggcgg ctggggcgct tcctacatgg
      961 cgcgcatcgt cgggcagaaa aaagcgcgtg aaatctggtt cctgtgccgt cagtacgacg
     1021 cgaaacaggc gctggatatg ggccttgtaa ataccgtggt gccgctggcg gatctggaaa
     1081 aagaaaccgt gcgctggtgc cgtgaaatgc tgcaaaacag cccgatggcg ctgcgctgcc
     1141 tgaaagctgc actgaacgcc gactgtgacg gtcaggcggg tctgcaggag ctggcgggca
     1201 acgccaccat gctgttctac atgacggaag aaggtcagga aggtcgcaac gctttcaacc
     1261 agaaacgtca gcctgatttc agcaaattta aacggaatcc gtaatgcgta gcgcgcaggt
     1321 ataccgctgg cagatcccca tggacgcggg ggtggttctg cgcgacaggc ggttaaaaac
     1381 ccgcgacggg ctgtatgttt gcctgcgtga gggcgagcgc gaagggtggg gggagatctc
     1441 cccactaccg ggcttcagcc aggaaacctg ggaagaggca caaagcgggc tgcttgcctg
     1501 ggtaaataac tggctggcag gtgatagcga gctaccgcaa atgccttccg tcgcctttgg
     1561 cgtaagctgt gcattggcgg agctggcaga cacgctcccg caggcggcca attatcgtgc
     1621 ggcaccactg tgtaatggcg atccggacga tctgatcctg aaacttgccg atatgccggg
     1681 cgagaaagtg gcgaaggtca aagtgggatt gtacgaagcc gtgcgcgacg gcatggtggt
     1741 caatctgttg ctggaggcaa ttccggatct gcatttgcgt cttgacgcga accgcgcctg
     1801 gacgccgctg aaaggccagc agtttgccaa atacgttaac ccggattatc gcgaccgcat
     1861 cgcgtttctc gaagagccgt gcaaaacccg cgatgattcc cgtgcgtttg cccgtgaaac
     1921 cggcattgcc attgcctggg atgaaagcct gcgcgagccg gattttgcct ttgtggctga
     1981 agagggcgtg cgcgcggtag ttatcaaacc cacgctcacg ggcagcctgg agaaagtacg
     2041 cgagcaagtg caggcggcgc atgcgctggg gctgacggcg gtgatcagtt cttccattga
     2101 gtcgagctta ggtctaacgc aactggcgcg gattgctgcc tggttaacgc cagacaccat
     2161 tccgggactg gacacgctgg atctgatgca ggcgcagcag gttcgtcgct ggccggggag
     2221 cctgctgccg ttagtggacg ttgatgcact ggagcggctg ttatgatctt ctctgactgg
     2281 ccgtggcgtc actggcggca agtgcgggga gaagccatcg ccttacgtct taatgacgag
     2341 caactcaact ggcgcgagct ttgtgctcgc gtcgatgaat tagcctccag ctttgcgatg
     2401 cagggtgtgg ttgagggcag cggcgtgatg ttgcgggcgt ggaatacgcc gcaaacgctg
     2461 ctggcctggc tggcgttact gcaatgcggg gcgcgggtgc tgcccgtgaa tccacagctg
     2521 ccgcaaccgt tacttgagga attgctcccc aatcttacgc tgcaatttgc gctggtgccg
     2581 gatggggaaa acacgtttcc ggcattaacg tcgctgcaca ttcagcgggt tgaaggcgca
     2641 catgccgcta cgtggcagcc gacgcgtctg tgctcaatga cgttgacctc gggttctacc
     2701 gggctgccga aagccgctgt ccatacttat caggcccatc ttgccagtgc cgaaggggtg
     2761 ttatcgttga ttccgtttgg cgatcacgat gactggttgc tctctttacc gctgtttcac
     2821 gtctccggtc agggaattat gtggcgctgg ttatacgctg gtgcgcggat gacggtgcgt
     2881 gataaacagc cattggagca aatgctggca ggctgtactc acgcttcact ggtgccaaca
     2941 caactctggc gtttgctggt taaccgcagt tccgtttccc tgaaagcggt gttacttggc
     3001 ggcgcggcta tcccggtcga gttgacggaa caggcgcgcg agcaggggat tcgttgcttt
     3061 tgcggctatg gtctgaccga gtttgcctcc acggtgtgtg cgaaagaagc cgacggtctg
     3121 gcagacgttg gttcgccgct gccgggtcgg gaagtgaaaa tcgttaatga tgaagtgtgg
     3181 ctgcgggctg ctagtatggt agaaggttac tggcgtaacg ggcaactggt ttcactggtt
     3241 aatgacgaag gctggtacgc tacgcgcgat cgcggtgaga tgcataatgg caagctgacc
     3301 attgtcggac gtttagacaa tctattcttc agtggcggag agggtattca gccggaagaa
     3361 gtcgagcgcg taattgctgc acatcctgcg gttttgcagg tgtttatcgt ccccgttgcc
     3421 gacaaggagt ttggtcatcg accggtggcg gtggtggagt atgaccagca aaccgttgat
     3481 cttgacgaat gggtgaaaga caagctggct cgttttcaac aaccggtgcg ctggctgaat
     3541 ctgccgccgg agctgaaaaa cggcggtatt aaaatttcac gtcaggcgct aaaagagtgg
     3601 gtgcaacgcc agcaataatt aaccctccgt ttcacttctc atttctgcca ttccattgcc
     3661 gttgataaag agtttatctg ttaatctgta aattgatgtg aaaactctta gcaaacagga
     3721 taatgcaatg gaatggctgg tcaaaaaatc gtgctgcaat aaacaagata acagacatgt
     3781 aattatgctc tgcgatgctg gcggcgcgat aaaaatgatc gccgaagtga agagtgactt
     3841 cgccgtgaaa gtgggggatt tactctcgcc tttgcagaat gcgctttact gtattaatcg
     3901 tgaaaagctg cacacggtga aagtcctttc tgcaagctgt tatagccccg atgagtggga
     3961 acggcagtgt aaagcggcag ggaaaactca gtaacgttgt tttgttgtgg gcaggaaaat
     4021 cagcatcaac ccgctcataa tacaggctac tcccagtagt gctttcagcg aaaaagtccc
     4081 ttcccagccg ggaagtacca ttgaagcaat ccataccagc acataactca tactgagcaa
     4141 ggcataggct ttgcttagtg caagtttatg tagtgtttta taccaacaaa aaacggaaag
     4201 cagataaccc agcagcccga gtaacagtat ccgggcatcc aggccaaaag ctaacaacgc
     4261 cgcgataaaa tcccataagt gcgtcatcgg cggcagatga ctcgccgcaa aacccaggct
     4321 taattgcgca gcggaggcga taatgacgct gaataatccc cacatcaggc ccattacacg
     4381 gtactcccga ggatcacaat gccaccaata atgaacgcca ccccacacca gtgacgcggc
     4441 gataccggtt cgtgccacag ttttactgca gccagcgtca cccagacaaa attcagactt
     4501 aacatcgggt aggcaatgcc taccggtacg ttctgcaaga ccagcagcca gagcaccatg
     4561 gcaagaccaa gacaagccag cgccagtccc agccacagca cgatatgttt gcgccgtttg
     4621 tttatcgcca caaagcaggt tgcctgtttc tgacacaact gcccggcaac gctaagcaag
     4681 ctggcaaaga ctaatgttag ccagatcatt tgggacgata ctgaatcagc accagacgct
     4741 cctgacgatc gatggcatct gcgggcggaa tggcgagact gttgatatct tcattgcggt
     4801 caaccgaaag cacgagtgta ataatcccct cctgacgatg ttgattaagc cagtttgcga
     4861 actcatcacc gctgacaaat ctccctttcg catccggata attaaggccg tatttcaact
     4921 cacctgtctg gcgatacatg atgatgtcat cgcgttgcag gctccatgcc agacctgcgg
     4981 caacaccgac gctatcagta agaatatagc ggcttggctg aagtgattct tgtgtcatct
     5041 cgacaaaaaa ttgcggatgt tttccttcca tgactctgtc aggaattgaa aatcctacca
     5101 gcaacgccag ccccagcggg caaagcgcgg caaaagacca ggtcttttcg acgtttgtta
     5161 aggtgtacca gccgaaaaat gcccatagcg aaaaaatcga ccaggcacaa aagactttat
     5221 agctctcgaa ggtttgccac accggcgtgt tcattgctcc ccagggggag accacaaatg
     5281 tggcaataat gccagtgacg ccaaaagcga tgttaatcca gccattaatc cgcaacgcca
     5341 gaggattatt ttttgctgcc agcaaagcgt aatgcgccat cagcattgcc agaggagcaa
     5401 agcaagaaag aatataggtg ggcaatttgc ctttggcgac ggagaaaaac aacagcggca
     5461 ttatcgtcca gctcaacaaa tagacggttg cggaatgctt gcggtttttc cagcctgcgt
     5521 acagtgctcc ggggagtaat cccagccacg gcaggctacc ggcaatgatg accggcaagt
     5581 agtaccagaa cggagctcta tgttgggcat catccattgc aaagcgttga atatgctcaa
     5641 cccagaaaaa atagtgccag aagtcaggct cccgctgcgc tatcgccagt ccccagggga
     5701 gaaccgtcag tacgcaactg atgaccgcca gccagccgta aataaagaga tctttccagc
     5761 gtttttgcgt tgcaacccat ggaagcacgc ttaataccgg cacggcaagg gcgagaaaac
     5821 ctttggtcat cacccccatg ccgcaagtga ttcccagcag taaaaatccc gcgcttttgc
     5881 ctttccacgt ctgtgcctgc attgccagcc agaagctgca cattcccgcc accagccaga
     5941 aggcaataaa cggatcgagc acggcataag tgccaatggc atagacaata aacaatgaga
     6001 gataaatcac tgcggcgagt agagccagac gtttatcgcg ccataagcgc agagtaaacc
     6061 aggtcaccag cgcggcagtt aacagggtcg caaagataac gcctgcccgc acaccaaagt
     6121 tattcgcgcc aaatagccat tgcccaatgc tgttaatcca gtatccggca atgggttttt
     6181 cgaaataacg tagccctaac agatggggca caatccagtc gccggatgcc agcatttctc
     6241 gactgatttc cgcataacgc gtttcatcgg gttgccatag cagacgcgtg ctgatcggta
     6301 acaggtaata gcaggcaata aacgcgaaga ggccgataag gtaacgtacc gatttcatca
     6361 gcgactaccc gcaatttgtt ggcaacccag ccagccttca cgtccggcaa tatttccgcg
     6421 aacaacttgt ccgagcggca acatccctga taacagttcg cttaaagggc aaaatgtcac
     6481 gccttcctgg gctgcccgtt taaggagatc cacaaaatta tgctgataag cgcagccttc
     6541 aacttctgca tgaatggtat aaaccggcgt gcctttatct cgcaggatgc gattgagtaa
     6601 ccaaccgtta aaatcttctg ctttcacatc ccgaccaatc acttcatccc aggtgggtaa
     6661 ggtcaccgga atttgcgcag taccagggtt tcctgattcg agcagtggac gaaacggcat
     6721 cgccccacga caatcgctgt tgtagcgcaa atggaacgct tcttttgctt cgatcacctg
     6781 ctggtcggca cgccagccag cggctgcgga gcaggttacc ggttggccga taatttcttc
     6841 cagggtgcga aggccgcgtg cgatatcgtc gatcattgtt tgccgatccc aattaccgct
     6901 atgggcttgc caggcatggt gatcccaggc gtgcaggccg acttcgtgat gtttagccgc
     6961 ttcacgaatg atatcggcat tggcatgacc aatctcttta cccggccagg ccgtacctgc
     7021 cagtaaaata tcccagccat aaagcgatgc cgcgtttgag cgcagcatct tccacaaaaa
     7081 ctgtggcttc accagtcgcc agagatggcg gcccatattg tccgggccga cgctgaaaaa
     7141 aatgctggcc tgaatattat gcttactcaa gatttccagc agacgcggca cgccttcacg
     7201 ggtgccacga aaggtatcga catcaatgcg taagcctact ttggtcatga tggtttatcc
     7261 gtaagatcaa cggtgcgcag gaagaaatcc agcgtttcgt cgatggtttc ctgcatatca
     7321 attttcggtt cccagtccag gcagcggcgg gcattgcgga tgctcggttt acgatgctct
     7381 acgtcctgat atcctttgcc atagtagctg ctactttcga caacacgaaa gcctgcaaac
     7441 ggcgggaagt gatggcgcag cggatgtttt tcgaagctcg ccagcagcat ctcgcccagt
     7501 tcctcaatgc tcgcttcgtt ctcaggattg ccaatgttga taatttcgcc gtcgcagcga
     7561 tttcccgcat tttcgataat gcggtacaac gcctcgatac catcccggat atcagtaaag
     7621 cagcgttttt gttttccgcc atcaattagc ttaattggtg aaccttctac cagattgaga
     7681 atgagttgcg taatagcgcg ggagctgcca attcgcgctg cattaaggtt atccagtcgt
     7741 ggtcccatcc agttaaacgg ccggaagagg gtgaactgta aaccctcttt ttcgccatag
     7801 gcccagatca cccgatcaag taattgtttt gacaccgaat aaatccagcg tggtttattc
     7861 accgggccga cggttaaatt agaatggtcc tcatcgaagt atttatcgct acacatccca
     7921 taaacttctg aagttgacgg gaagatgatt cgcttacggt acttcacgca gtagcggata
     7981 atgcgcagat tctcttcaaa atcgagttca aatacgcgca gcgggttgcg ggtatattca
     8041 atcggcgtgg ctatcgccac cagcggcaag acgacatcac attttttgac gtgatactca
     8101 atccattcgg aatgaatact gatatcgcct tcgacaaagt gaaaatgcgg atgattcaga
     8161 aaacggctta tcgcatcgct gccaatatcc agaccgtaaa cttcataatg atcttcgcgc
     8221 agcaggcgtt ctgtcagatg gttgccaata aagccattca ccccgaggat gagtacccgg
     8281 gtacggcgtc gggcggcaca ggcaggctgg ctattcaagc gtgaaccttg caccaggccc
     8341 agcgtctgcg ctaattgcga gccctgcata gtaatgccgt cgcccgcctg tccggtgacg
     8401 atttccagcg cgccatcgcc acaggcaatc agcagtggcg ctacagaaat cacgctcccc
     8461 ggctgtgctt tgctggcatg aggatgaaca cgcgacgacc agacggtgaa tttctgattg
     8521 ccaacatagc tgaaggcacc cggccacgga tcggcaacgg cacgtaccat gttgtgcagt
     8581 acggatgccg gcttatgcca ttcgaggaaa ctgtcatccg gcgttctgcg accaaaacag
     8641 gtagcttcgt tttcgcgctg ggcgatttcc agaatattac cgtgtttaat ggtgggtaat
     8701 gtctgctcca gtagctggcg cgcggcatga cataacttat gatgcaacgt aatggcgata
     8761 tcgtctggcg caatggcaat gcgcagttgc gccacaatgg ccccggcatc ggcacgtttc
     8821 accattcggt gcaatgtaac gccagtttcc gtttcaccgt tgaccagcac ccagttcagc
     8881 ggcgcgcgac cacgatattt tggtaacagc gaaccatgca gattaaatgc acctgcggga
     8941 gcgagctgca aaatctcgtc gcaaataaga tggcgataat aaaaagagaa aatcacctct
     9001 ggtgagagtt gggcaatacg ttccacccac agcggatgat taacgttatc tggcgcataa
     9061 accggaatgc ctctttccgc cgccagacga gctaccgaac cataaaaggc tttttcaccg
     9121 ggattatcgg tatgagtaaa aatagcgcta atttcgtaac cggcagccag cagggcttca
     9181 ataccgaggc atcccatatc gtggtaggca aaaacgacgg ttttcattat tcattttcct
     9241 tgctggatgg acggataact tgctgaacaa aatagcgggg gcgggcgcgg acatcggtgt
     9301 agatcctgcc gatatattca ccgagtaatc ccatgccgat aaactgagcg ccaataaaag
     9361 taaacagcac ggcaaatagc ataaagacgc cttctgccgc ccattgtggt ccgaaggtca
     9421 ggcgtaaaat caccagcagc accgcaatgc taaaacctcc aatcgcaata atgctgccga
     9481 gcagactcag catacgtagc ggcgttgtgg taaggcaggt caccaggtcg tacatcaaat
     9541 taatcaggcg cataaaactg tatttggatt caccaaactc acgctcggca tggtgcactg
     9601 gaatttcaat ggcacggcgg gcgaagatat tcgccagaat cgggataaag gtactgcgtt
     9661 catggcagtg caacatcgca tcgacaatat gacggcgata ggcgcgcagc atacaaccgt
     9721 agtcacccat cgctttgccg gtggtgcgct gaataagccg gttaatcatc ttcgaagcgg
     9781 ttttacgaaa ccagctgtcc tggcggttct ggcgtacagt ccctaccacg tcgtaacctt
     9841 catcggcttt tgccaccaga cgggggattt cctctggcgg attctggaga tcggcatcga
     9901 gggtaataat taagtcgcca gtaacgtgac tgaatcccgc cataatcgct gaatgttgcc
     9961 cgtaattgcg gttaagcaaa atagacacaa tatggctgtt ctccgcttgt gaggcttcga
    10021 ccagcatatg cgcggaatta tcgctactgc cgtcatcaat cagcaggatt tcatactctt
    10081 tccccagtga ttcacaggct gcggtggtgc gcctgattaa ttccggtaag ctttcctgct
    10141 cgttataaac gggaataacc accgagactt tcttaacagg gtggatttca aacatggctt
    10201 attgtcctgc gagttgctga agggctgtga tgacgcggtc ggcatcggcg gtggtcatat
    10261 ccgggaacag cggcaacgaa cagatgcgtt cgctattcca ttcggtattc ggtaacgaca
    10321 gcgtgggaaa acgctcgcga taatattttt gtgtgtgagc ggcgcggaaa tgtaacccgg
    10381 taccaatgcc tctttctttt aacgcttcca tcaacgcatc gcgactgata ccacaacgtt
    10441 gttcatcgac acgaataata aacagatgcc aggcgtgaac gtgtgaccag gcgggaaggc
    10501 ttaatggctg aaaggggaga gctgccagtg cttgctgata ttgctgggca atttcgcgcc
    10561 gacgggtgtt gaggtgctct aatttgacta actgtgtcag ggcaatcgcg gcgttaatat
    10621 cggtcagatt gtacttatag cccggcgtta agacttcagc ctgcggtgca cggccccagg
    10681 tatgtctgtc ataggcatcg acgcccagac cgtgaaattt tagcatccgt agctggcggg
    10741 caaggttttc attatctgta acaatcaggc caccttccgc acaggtaata tttttgatgg
    10801 cgtgaaacga aaaaatagcg gtactttttg cgccaatatg tcgccctttg taatacgtac
    10861 ctacggcatg ggcagcatct tcgataactg cgatgccgta acgttcgcca atggcgcgaa
    10921 tggcgtcaat atctgctggc gcaccggcat aatgcaccgg aatgatggct ttagtgcgtg
    10981 gcgtaatggc tgactcgatc gcttcaggcg tgaccatcag cgtatcgcgg tcgacatcca
    11041 ccattaccgg cgttgcaccc aacaaggaga tcatattgag ggttgaaacc caggtcaggg
    11101 aaggcgtaat cacttcatcg cccttgccaa tttccaacgc cattagcgtg atatgcattc
    11161 cggcggtggc tgaactgacc gcgatggcat gctgatttcc cgtcaactgg caaaaggctt
    11221 gttcaagcgc ctgattcttc ggaccggttg tgatccaacc ggattcgaga acctctttaa
    11281 ctgctgcgag ttcctccacg cccattgctg gtcgcgaaaa aggcaaaaat tctgacattg
    11341 cttttccttc cgccattgaa tacctgtcca cttatatttg ctataaagag tgttgtgtat
    11401 attttgccat ttggagc
//
LOCUS       DAZUCC010000161        11401 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZUCC010000161 DAZUCC010000000
VERSION     DAZUCC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.162"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..341)
                     /locus_tag="ACHY09_003682"
     CDS             complement(<1..341)
                     /locus_tag="ACHY09_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS3886599.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISTTSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLAR"
     gene            881..964
                     /locus_tag="ACHY09_003683"
                     /pseudo
     CDS             881..964
                     /locus_tag="ACHY09_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1126..2184)
                     /locus_tag="ACHY09_003684"
     CDS             complement(1126..2184)
                     /locus_tag="ACHY09_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS3886600.1"
                     /translation="MANTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHLQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(2335..2532)
                     /locus_tag="ACHY09_003685"
     CDS             complement(2335..2532)
                     /locus_tag="ACHY09_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS3886601.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(2749..3309)
                     /locus_tag="ACHY09_003686"
     CDS             complement(2749..3309)
                     /locus_tag="ACHY09_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFS3886602.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRRMKKSGLDKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKLKMAERLNADHPEWTLVTCRRRIDQWL
                     GISEFMLHAPMRMAFVTEKKMLQTDQ"
     gene            complement(3318..3677)
                     /locus_tag="ACHY09_003687"
     CDS             complement(3318..3677)
                     /locus_tag="ACHY09_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS3886603.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYKIESE"
     gene            complement(3690..4739)
                     /locus_tag="ACHY09_003688"
     CDS             complement(3690..4739)
                     /locus_tag="ACHY09_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS3886604.1"
                     /translation="MRVLLRPVLVPELGLVVIKPGRELLPVFHRGRVLVEPEPKSMRS
                     LPSGVVPVVCQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            complement(4741..5013)
                     /locus_tag="ACHY09_003689"
     CDS             complement(4741..5013)
                     /locus_tag="ACHY09_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886605.1"
                     /translation="MTQHIKSHNSEADPEIKRGWRFRTPQYGWFHYLFCTTDEADMLQ
                     EAYLRRGVRVERSLNVDRLTWTVSVYLPVRAHLPRTNACYRQRVWR"
     gene            complement(5136..5480)
                     /locus_tag="ACHY09_003690"
     CDS             complement(5136..5480)
                     /locus_tag="ACHY09_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFS3886606.1"
                     /translation="MKKVLIAALISGVSFGVFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIIKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            complement(5602..5757)
                     /locus_tag="ACHY09_003691"
     CDS             complement(5602..5757)
                     /locus_tag="ACHY09_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3886607.1"
                     /translation="MKQQKAMLIALIVICITVVVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(6048..6401)
                     /locus_tag="ACHY09_003692"
     CDS             complement(6048..6401)
                     /locus_tag="ACHY09_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS3886608.1"
                     /translation="MIDRTKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMCKAGN
                     SPVTPDGWISCSERMPPVGVDVQLFVSSTGEQFTGFNLDNTGDFQYAQCFGAPIVCEA
                     THWMPLPEPPKEVRQ"
     gene            complement(6416..6766)
                     /locus_tag="ACHY09_003693"
                     /pseudo
     CDS             complement(6416..6766)
                     /locus_tag="ACHY09_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6768..7112)
                     /locus_tag="ACHY09_003694"
     CDS             complement(6768..7112)
                     /locus_tag="ACHY09_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS3886609.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALAAFK
                     CEIRHREVDIIADLLDELEEAKQRIDEQESRIVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTVAGIRIEGE"
     gene            complement(7109..7759)
                     /locus_tag="ACHY09_003695"
     CDS             complement(7109..7759)
                     /locus_tag="ACHY09_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS3886610.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLSEVRALGVDA
                     AIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLIGKVEMTEWLVDFAAQLRKGGNQ"
     gene            complement(7746..8051)
                     /locus_tag="ACHY09_003696"
     CDS             complement(7746..8051)
                     /locus_tag="ACHY09_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFS3886611.1"
                     /translation="MRVSIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            complement(8048..8329)
                     /locus_tag="ACHY09_003697"
     CDS             complement(8048..8329)
                     /locus_tag="ACHY09_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3886612.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            complement(8362..9078)
                     /locus_tag="ACHY09_003698"
     CDS             complement(8362..9078)
                     /locus_tag="ACHY09_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS3886613.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGAMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVADTDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATRELLR"
     gene            complement(9112..9573)
                     /locus_tag="ACHY09_003699"
     CDS             complement(9112..9573)
                     /locus_tag="ACHY09_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFS3886614.1"
                     /translation="MTEQQIRLVCRQCMDRCWAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(9566..10597)
                     /locus_tag="ACHY09_003700"
     CDS             complement(9566..10597)
                     /locus_tag="ACHY09_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS3886615.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDTE
                     LNPTHNARESIPTSESNGALLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVW
                     GMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGGSHA
                     APGGTASRAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEA
                     LDCPDWCDD"
     gene            complement(10666..11091)
                     /locus_tag="ACHY09_003701"
     CDS             complement(10666..11091)
                     /locus_tag="ACHY09_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS3886616.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTSDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAVAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(11095..>11401)
                     /locus_tag="ACHY09_003702"
     CDS             complement(11095..>11401)
                     /locus_tag="ACHY09_003702"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3886617.1"
                     /translation="SKCAEIKIMTLKEFIKSLKTGEAKKFATNLGVSPSFLSQMASGT
                     SPVSPVRALMIEAETGGHVTRRELRPNDWELIWPEYTGITSPSRHKYELNTKKADGG"
BASE COUNT         2612 a         3338 c         2535 g         2916 t
ORIGIN      
        1 cgcgccagct gcttaatacg cgggtccgga cggtcatatg tctccggcag cggaaggcct
       61 tcaccatacg acatgccgtt tgactgccct gccagaacca caacaaagta atactccggg
      121 tcgctggtgg tgctgataac tgtgccttct ccatccgacg gcttcaccag cacaggtgtg
      181 gtgacatcac cttccgccgc aatggcctgc atcagggtat aaggcgtgat ggcgacagga
      241 ctgccaaatg gctgccaccc ctccttcagt ttttgagtca gtcgctccgc aaggtctgac
      301 ggcgacgccg ccctgaccac gtcatagtgt ttaaatgcca tgaatcctcc cggccgggat
      361 aatgttgtga gtcagataag gggcgggctg aagtccggaa gttacaggac aatgacagaa
      421 ggaagactac agcccgcaat acgaaaaagg ccgcgcagtt gcgcagagtg attactgtcg
      481 ggtattattc gccagctgaa aaattgatta cttcacgttt tgttgtttat tccttgctgc
      541 ccgcgtctcc cggcgcgggc tttttttgca tgtaaaaagg ctcctgcgat gaggagcctg
      601 gatatatgcc taatctctgt atacagcatg atgccgggtg cctcccggtg aattctgcaa
      661 tgaccagaca gaatccgcaa cttgcctata caatacgcaa ccaaacatct gtcattatgc
      721 ccctccgctc agggggattc atcatacagg atttttttaa caaatgctca gccagacagg
      781 caaccgtcaa ctgactgaat tgtgagtgat ttaacatttc actgcccggt gccttcccct
      841 cattaaaaag cccctcagga gaggggctaa agccgcgtat ctgtatcatc atgcgcatgg
      901 tgccgggtgc ctcccggtga gttcagtatc agcacctgaa cccgcacaga aaggataagg
      961 gatataacca accacctgtc gctgatatgc ccctccgcac agggggattc accatgcctg
     1021 ttccttttaa caaacgcccc gcaaaacaga caactgtcaa ccgtctgaat tgtgagacat
     1081 ttaaaatttt cggggcgtaa ctgatacccg gttaattctg caatatcatc cgttctgact
     1141 gactaaatcc tgaacttccc tgacagtctg ctcaaaacgt tcagtctcca gctcaacgcc
     1201 agttgcacga cgtcccagcg ccatcgctgc tttgactgtc gaacccgacc ccatgaagaa
     1261 atctgcaacc aggtcacccg gacgactgct cgcgctgatt atctgctgca gcatttctgc
     1321 cggtttttcg cacggatgtt tcccgggata gtactgcacc ggtttatgcg tccacacatc
     1381 cgtgtacggc acctgcgccg tcacgccaaa ataccgccgc agatgcttat attcactctg
     1441 cagttccgca tactgccggt tcagtgaagt atacgtctcc agcagctgga ggtggggctt
     1501 ttccagttca ccccgctgat gcttctcttc tgccacccgg gcaaacagcg cctgtaattt
     1561 cagataatca ctttcgtccg gcagttgcca ctgactggca ctgaaccagt gcgacaccat
     1621 gtttttcttt cctgtggcat ccacaatctg ttttgccgtt atccccaggg ctgcgcgcgc
     1681 atcacgaaag taagcaatca gcggtgccat cacatgctgt ttcagtgccc tgcccttcgc
     1741 ctcatacccg gcatctttcg gacgatacgg cccctgataa tgttccgcga acagaatgcg
     1801 ctctgtggcg gggaaatacg cccgcaggct ttccttgttg cacccgttcc agcgtccgga
     1861 cggcttcgcc cagataatat ggttcagcac actgaagcgt tcacgcatca tgatttcaat
     1921 gtcagacgcc aggcgatgac cacagaacag gtaaagactt ccggcaggtt tcagcacccg
     1981 ccagaactgc gcaagacact ggtccagcca tttcaggtaa tcctcatccc ccgtccactg
     2041 gttatcccag ccctcgggct tcactttaaa gtatggcggg tctgtgacta tcagatcgac
     2101 agagttttcc ggtaaggtct ggataaattc caggcaatca gcgttgatta gctcacaact
     2161 ggatattttt acagtattag ccatagatca ataagcactt ctctgatagg ctcattctgc
     2221 ttttgcgcaa agcagtgggc ctgaggtttg cttgtgatcc ggacgcatga gcagatggct
     2281 ggtgagtgcc cctaacaccc accagccgcc catttaccac aaataaaaaa gccttcagga
     2341 ctgaaggcgt ctgtaacaac cgaactgata gtctgccaga cccgccataa caagctgggt
     2401 cagtattaac tgacagcgtt cgcgtgaaag gtacgtattc tgtgcaatct ccccgactgt
     2461 cgccggttcg gtgacgctta attcattaaa caccactctg gcggtttctg tcatatcctg
     2521 ctgtttcagc atgtcttttc cccttttccg gttaacgtga cacaccaata actcttgtcg
     2581 aaaaagccag caagctgaaa gaacggtatt aataaccacc agcgaattta ttgcgctgct
     2641 gtatattacg gacacaaaaa aaccaccttc cggtggcttc cttgtgcgaa aaaacttgcg
     2701 tttcgcctcg cgatacagcc ttgcgaagct tatacggatt gaagcagttt attgatcagt
     2761 ttgcaacatt tttttctctg taacaaaagc catacgcatg ggggcatgta acataaattc
     2821 agatatcccc aaccactgat caattcggcg tctgcacgta accaacgtcc actcaggatg
     2881 atctgcattt aacctttcgg ccattttcag tttgctcatt ccccgtcctt cgtacctttg
     2941 ccggagaata ttgattagtc cgggatattc cccaagcacc tcactgataa cgcgatcaat
     3001 aatcaacgcc tctgtgtctg tacaatgtga caaccagctc ttctgcttcc ctctggtcat
     3061 atcccgaaaa aatgcctcaa gttccggttt atccagcccg gatttcttca ttctgcgtaa
     3121 aacttcatta actgctgttt tcgtcagctt tttcgaaacc agtaaccggt taaacatatt
     3181 tccggattta cccccaccga tatacgacca ccgcccccac atccgtaatt tcccctggat
     3241 ccagactgct tccagcgtgt tcaggcgtaa atgttcgccg cttttgcctg taatttccgg
     3301 atatatcata tttacgctca ctcactctca attttgtaaa tcttcacacc cagccgacca
     3361 ccaggaacaa gctgaccacg tacaatattg atttcatcaa actgctcgtc atcaatgagc
     3421 actcccgcat gcgtcagcgc atccagcggt gctttcagaa tgttgtccag gtcacgacga
     3481 cgcttatccg gtggctctgc aataattttt attgccagcc gtccggacag gcttaatttc
     3541 agccgctgct ggcgaacaat aagcgccact gcacggcgat aacgctcccc ggcttttgat
     3601 acaaaatatg tgctgccacg gcgtcgccag taagtgttca ccgtcggcgg gtaaggtaaa
     3661 accaaatcta tgagcatcag tcacctcttt tacccgagca cgccagtcgc aaaggcgtga
     3721 tcaagaaaac gaaaaattaa ctcaatctga gagccgtact ttttctcaaa ctccagcggg
     3781 tctgcatgaa gttcgttgtg gtgctcccgg cacaacggta gcgtgaaaat atcgtgggcc
     3841 tttgtcccca ttcccccctg accatgacca atcaggtgat gcggatcgtc agcaggctta
     3901 ccacaacacg cacacggctg tgtctttacc cagcgcgtgt atttctcatt aacccaacgg
     3961 cgacgtttag gccgcctcat gaacgattca ggagactccg gatcaacggc gatactgaca
     4021 accgtttttt tctgtggtgg attttgttgc tggtggacgt gaagtggcag cgcaatattt
     4081 tttgtgcgct gcttcagtat gctgatggct gtctgttctc ccggtacgat gtcactctca
     4141 cggtatacgg agcggatttt ttccgctggt aatcccagcg aacgacgcgc tactgcctca
     4201 ggtagtgcat ccaccacctg attgcaggcc gcccaccagg ataattcggc cagcgataac
     4261 tccctctcct gcgtgccgct tattgcgtga cggatgacgt caatcatcca ggcaaccaga
     4321 ttctgctgag caagttgatc gagtgattct gatgtctggt cgcgcagctg gttgtcacag
     4381 tgccagcaca acaccatcgc gccggtaccg taacggtgaa tgacggtttc gctgtgatga
     4441 taatcgccat gtggccactg gcaggatttc acgtgacgta ataaccagtc agacagcgca
     4501 ccagcaccgc cagcggcacg aatcacccgt tcattgctga aaaatggcag taatgattta
     4561 tcctcagcca gcggctggca aacgacagga acaaccccgg acggcaaact ccgcatgctt
     4621 ttgggttccg gctccaccag caccctgccg cgatgaaaaa ccggcagtaa ttcgcgacca
     4681 ggcttaatga ccaccaggcc aagttccggg accagaacag gtcgaagtaa tacccgcaca
     4741 ttatctccag acacgctgac ggtagcaggc atttgttcgt ggcagatgtg cgcgaacagg
     4801 aagatataca gaaacggtcc aggtcagacg atcaacgttc agactccgct ccacacggac
     4861 accgcgacgc agatacgcct cttgaagcat atctgcctca tcggtcgtac agaacagata
     4921 gtgaaaccag ccatactgag gcgtacgaaa acgccacccc cgcttaattt ccgggtcggc
     4981 ttcagaattg tgggatttta tgtgttgtgt catcggattc tccggtgaca gcaggtgtca
     5041 gttgttcagg ctgacggcgc gaattgtaag tcaatacgcc tggaatgtac aaacagaaaa
     5101 cccgtcagta agacgggctt agcaagccgg gacggttact ttaataattt cagtgccttt
     5161 acatcaactt caacactgct caggtcttta tcaatttcac cctcaattct tactttgtct
     5221 ttcggagaaa cattctgccc ggcccatacg ctgtcatcaa tatccgtgac aattgtcccg
     5281 ctattgtcac gaaactcata acgttcatca cccacttttt taatgatgct cccttcaagg
     5341 ataacccatg catcatcctt cagttctttt gcctgcgcta ctgttgaacg ctctgcttca
     5401 ggcccctgga aaccaccctg ctgtgcaaaa acgccaaaag acacaccgga aataagtgct
     5461 gcaatcaata cctttttcat tcatagtcct ctttcagaaa tgaacattca aacagcattt
     5521 tcagtatggt aaagcgcggg tgcgttgagg atgcctgaca catcagaggt ggcgagggat
     5581 ttctccctcg cctggtcact cttacttctc agattcgtag tctacgaaga cagcaacctc
     5641 cgtctggccg gttcggattc gtacctcgca gaggtctttc ctcgttacca gtgccgtcac
     5701 aacaacggta atacagatga cgatcagggc gattaacatc gccttttgct gcttcatagc
     5761 ctgcttctcc ttgcctttcg gcacgtaaga ggctaaccta cgtttgtgaa gcatagattg
     5821 ggcctcagat taatgttaag cgtcctgcaa gacgcgtaat gttaactggg gcttttctct
     5881 gtctgcctta cggcggcatg cccgaggcag acagcctcaa gcacccgcag caattctact
     5941 taactcttct ttccccgcaa accgttttta tccccagcgg caaatcgaat acaccaccag
     6001 cgccaccgcc attgcaatac caacatttga gaaggcctca ggccagctca ttggcgcacc
     6061 tccttcggcg gttctggtag cggcatccag tgtgtggctt cacacacgat aggcgcaccg
     6121 aaacactgcg catactggaa gtcacccgta ttatccaggt taaaacctgt aaactgttcc
     6181 cccgtgctgg acacaaacag ttgtacatca acaccaactg gcggcattcg ctcactacag
     6241 cttatccaac catccggagt taccggagaa ttgccagcct tgcacatggc aatctccatg
     6301 atttcaacca tatctcctgg tggaatttta caatgctgac caatatgcct ctgctgcctg
     6361 gcatattcga ggatgtgctc cagtttggtt cgatcaatca tgatttatct ccattaagca
     6421 ttgcagcacg gcaatcgttc catccttcag cataatcact atgcgcaaga ggccaacctc
     6481 ttatgtattc acgcggcgac ttatcaggca ctaccagcgc tggcgggacg gcaaatagat
     6541 atccgccaaa gtcaggaagc tctccaatgg cctgtacgaa cttttgtttg cctacgtcaa
     6601 ctcctaatgg gtaatgagct ataatctttg ccactggctc tgcttccagc gatgccagtg
     6661 cgataagcgc cagctcacga atttcaccgc cgtctatatc gtcaatgtca tcgcggccag
     6721 aaatgttagc cagccattgc agtcgctctt tggtaatagt ggtcatctca ctctccttct
     6781 atgcgaatac cagcaacagt cagtgcggca ttgacctcat cagcgtagta gtacgttaat
     6841 ccacttgatg atttagccag cttgaatggt tctggtaact tcactatgcg ggactcctgc
     6901 tcgtcgatac gctgttttgc ttcctccagt tcatccagca aatcagcgat aatatccact
     6961 tcacgatgac ggatttcaca cttaaacgca gccaaagctg catcacaatc ctgctcagcg
     7021 tttggactgt ctggtttctc ctgataccac gccagcatcg accgatagtt ttgtgctgcc
     7081 tcacgcaatg cctgatagtc aatcttgctc actggttgcc tcctttgcga agctgggcag
     7141 caaagtcaac taaccactca gtcatttcaa ccttccctat caggtctgaa ccagggtgca
     7201 tacagcaatc actctgcgcc gctttgaaat ccttatactc atattcttgg gccaccagat
     7261 tttttgcagc ttctatagca gcatccaccc ccagcgcccg cacttcagac aggaaagcat
     7321 cggtggctgg catatttcct gttgccttca tggcctccaa aataaccaga acgccatctc
     7381 tcccaacctc ctcgcagata acctcggtgt tgtcgccaac aacatcgcag aatgcctgaa
     7441 ctgctttacg agcaagtgca ttctccgccg ccagcgccac aagattagct tccagctcgg
     7501 caatacgttt gctttgggct tcccgttcat ccagcagtgc ctgtactact tcagggttga
     7561 aagctgcaat aaattctgcg ttgttctctg catttttctg gtcatcaaag ccaggccatt
     7621 tgataatgtc tccgcaacgt tcatcccccg gcgtatgcac cgcatatgta tcagtgcccg
     7681 gcgaaatgaa tgcgacccat tcgccctggg ttgccagttt tgctatctca cgtagcgtct
     7741 gataattaat ttttctcact ggttgcctcc tggaaaataa ccgcatgtcc cagtttctcc
     7801 gccagtgcca gttctgcctt agcgcctgcc gaccgctgcc agcctttcag catataaacc
     7861 gcatccacac aacgaatcat tgccatgcaa atatccatgt actgcggctg agtcagcccg
     7921 tccggaagta ctgccgggtt taaaacggta tgcccttccc gtttcagtgc ttcttccgcc
     7981 ctgtgaaatg cctcgcggtt gaaattttca tatcccgtca ttggaccggc gatataaatg
     8041 ctcaccctca cgcctgaacc ctcctgtcga aataaacgta gttattcact gcgcccaact
     8101 tcatcccaaa cttttcggca atttcccgtc ggggtacgcc acgctgatgc agttgccgcg
     8161 ccagctcaat atcacgctgt gaacatttgg ctgactggtg ataatcaccc cgtaacatca
     8221 tgcagatacc cagttcccgc gctttcgtcc tgacggcagc cccactacgg ccaatcagac
     8281 tgccgatgct ttcgactgtc atcgttcccg cacactgccg gagtatcata atttccgccc
     8341 agcgccactt cttccagcca ctcaccgcag cagctctctg gtggcggtaa tatcccggag
     8401 aatatcccgg cacttgttca gctcccgcag cgcggcgcag actcgctccc acttctgaac
     8461 ctgacctttt gcccggcgca gctcgcggtt agccacatgc agcgatggta aaatcagacc
     8521 atccggatgc tttctggtga acgacggctg tgactgcact gtgaccgcca cactttcagt
     8581 ttttatttct tcctgtgttt ccgcttcccg gactggtaac gcaacacctg ctggctgagg
     8641 aaaggcttta ccatcggtgt ccgctacgga tgcagcttcc ggctctgccg gtaaatcagc
     8701 gcccggtatg cagtaacgaa atttaccgcc ctgattcacg cgaatcagac gccctttgct
     8761 gattgccatg gccagcgatg aattcgcccg gcgggaggta atcccgaaca taagtgccag
     8821 ctcatccgcc gtttgtgggc catgttgttc aatcgcctca gtcagcattt gcgctgtcac
     8881 tttcggtacc ggtgacactg gttcactttc accagcctga atcagccacc acatcgcccc
     8941 cttgttatcc gcttcaccgc ggcgcttcag tttccacagt tcgttgaccg catcttcacg
     9001 gctgattcca aggcgggccg ccactacctg tgaagaggct cttttcagtg ctttcagtgc
     9061 gtcaaatacg gtttccatta aaatttcctc cgacaaaatc gtttctcaga ttcaaataaa
     9121 accagctgcc ttccggcgtt cgtattcctg tttcagccgt tcaattggcg ttggcccttg
     9181 cgggtgtttc gccccttcca gttgtcgtcg cactggcgga acactcatcc cgttaccaac
     9241 atgctttgcc catttcgtca gttgccgttc cgcaagtcgt tttaactcac cctgcgtcat
     9301 ctggcgctca atccctctgg tacgcatttc gaggcaaatg tggtacagca caggctgtgg
     9361 ccacgggtat ttatcactcc cgtcgtatcg ccaggattca ttgcgccagc gccggtactc
     9421 ttccatcacg gcatccaccg taagaccaaa tggatttgcc ccactctccg aaatcagtgc
     9481 aacaaactca gccaggtccg ggggccacgt ttcacccgcc cagcagcggt ccatgcactg
     9541 acggcagacc agacggattt gctgttcagt catcgcacca atctgggcaa tccagagctt
     9601 cgaaggtgcg gccccgttct tctgagtcca gcggttcgaa tacacctccc ccataagctc
     9661 ccacagcttc caggccgttt ccgttgctga taaatccgtt ttcacgttcc cactgttcgc
     9721 gtgcagcccg gatttcctga actgcccgtg atgcggtgcc acctggtgct gcatgacttc
     9781 cccccttgct gactggtttt acctgtgccc tgacgtgctg cacgtggcgg gcaaatttct
     9841 gctcccactg aacctgcgtg aaaaccttcc cctccgccat ccagtaatcc cggaatgcgg
     9901 caagctcagc aggtgtaaat tccggctcag gcagagccat accccacact gctgcccgtt
     9961 gtcgaaaatc cggcgacggc tgccagacag tagtcatcga aaatttcccg atcggttcgc
    10021 tcaggccgtc caggtattca ggttcggctg tctgcaacag cgcaccattc gactcactgg
    10081 tcggaatact ctcgcgcgcg ttatgtgtgg ggtttaattc tgtatctgta tctttatctg
    10141 tcgtgacttg tcgtgacaga tgcgtgacac gtcgtgactc atcgtgacaa tcagcattat
    10201 gtttccgcag cttttcccgc tcccgctgcg ctctcttacg ctctgccggg gattttgccg
    10261 tttgcgaaac gttaccattg tcctctttca gcacctgacg tttttcccat ccggaaataa
    10321 ggtcaccatc cagaacccgc ccctgcattg catgcaaaat tgaatcaatt acgtcttccg
    10381 tcacatcaag cgcacttgct aaatcttccg tcgtgacatc aatgtgacca cgtagtgaca
    10441 cgccgtgaca cgtcgtgaca tttcgtgacg cgctcaccag aaggtggata tacactgcca
    10501 tcactgttgc gattggctgt cctgataccc tggcaatcgt tcgccacttg gggtcatttg
    10561 gcatgtcatg ccacaatctg agccaggcat tagccatact cacctcttct gataccgaac
    10621 tttacccacg aacttccgga agaaatccgg tataaatatt gttggtcaat gcacaacaac
    10681 agcattacca ggctgaccac cactgttagt cagggtgccc caggcgatcg ctacagcgac
    10741 aaaatcatcc acatctttca ccagccgatc ccgtcgttcg acgatctccc ggtaatattc
    10801 agaactgtga ctgcgcatac gggccaccag caaaggcggc atcgcctttt cgatcgccgg
    10861 taacagcgcc tgaatttttt caacagcatc agacgtgtcc ttctccaccc agcggaaaat
    10921 tttctgggta ttacgagcca gggcttccgg atggctgtcg tcgtacagtt ccgggaacgt
    10981 catccccagc tcgaaataag tccgggctat ttcagctgcg ggaactttct caccatcagg
    11041 atgtgcccag gcattcatcg ccatgcggat gtgttcatgc ttgattttca tgaatcaacc
    11101 tccatcagcc tttttggtgt taagttcata tttgtgacgt gaaggggacg tgattccagt
    11161 gtattcgggc catataagct cccagtcatt aggtctcaat tctcgtcttg taacatgtcc
    11221 tccagtttct gcttctatca tcaacgcacg aacaggagac acaggtgaag ttccagatgc
    11281 catttgggac aagaaagacg gagacacacc aagattggtc gcaaattttt ttgcttctcc
    11341 agtttttaat gatttgatga attcctttag cgtcataatt tttatctcag cacatttaga
    11401 a
//
LOCUS       DAZUCC010000162        11387 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZUCC010000162 DAZUCC010000000
VERSION     DAZUCC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.163"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..805
                     /gene="gspC"
                     /locus_tag="ACHY09_003703"
     CDS             <1..805
                     /gene="gspC"
                     /locus_tag="ACHY09_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFS3886618.1"
                     /translation="RFPFHLANHNKDAAINILIIFISIGLIIFNVNYFHTTIVKNGQI
                     INQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSNDETSF
                     VLINEGSEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDIIKQP
                     DSESQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNLSLTH
                     PDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRHERTQ"
     gene            789..2741
                     /gene="gspD"
                     /locus_tag="ACHY09_003704"
     CDS             789..2741
                     /gene="gspD"
                     /locus_tag="ACHY09_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFS3886619.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNERKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHTPAPSAR"
     gene            2751..4232
                     /gene="gspE"
                     /locus_tag="ACHY09_003705"
     CDS             2751..4232
                     /gene="gspE"
                     /locus_tag="ACHY09_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFS3886620.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLENEA"
     gene            4229..5425
                     /gene="gspF"
                     /locus_tag="ACHY09_003706"
     CDS             4229..5425
                     /gene="gspF"
                     /locus_tag="ACHY09_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFS3886621.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTAVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5435..5872
                     /gene="gspG"
                     /locus_tag="ACHY09_003707"
     CDS             5435..5872
                     /gene="gspG"
                     /locus_tag="ACHY09_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFS3886622.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5880..6389
                     /gene="gspH"
                     /locus_tag="ACHY09_003708"
     CDS             5880..6389
                     /gene="gspH"
                     /locus_tag="ACHY09_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFS3886623.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6386..6763
                     /gene="gspI"
                     /locus_tag="ACHY09_003709"
     CDS             6386..6763
                     /gene="gspI"
                     /locus_tag="ACHY09_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFS3886624.1"
                     /translation="MNKQSGMTLLEVLLAMSIFATVALALMSSMQGQRNAIERMRNET
                     LALWIADNQLKSQDTFNDENTSSSGKEIINGEEWNWRSDVHSSKDGTLLERTITVTLP
                     NGQKTALTRYQSIHDRSGQAQDD"
     gene            6756..7343
                     /gene="gspJ"
                     /locus_tag="ACHY09_003710"
     CDS             6756..7343
                     /gene="gspJ"
                     /locus_tag="ACHY09_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFS3886625.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLGVLAFMVFSQASTLHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPQAPLNEAQTL
                     QTVHWYLRNHTLYRAVRTSVDGRKNQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7336..8319
                     /gene="gspK"
                     /locus_tag="ACHY09_003711"
     CDS             7336..8319
                     /gene="gspK"
                     /locus_tag="ACHY09_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFS3886626.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQFWPQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8334..9497
                     /gene="gspL"
                     /locus_tag="ACHY09_003712"
     CDS             8334..9497
                     /gene="gspL"
                     /locus_tag="ACHY09_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFS3886627.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTPTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMRCYGVAPHGVAATNPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QHQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMQKTEKDPVSGVWTVRSSGK"
     gene            9494..9955
                     /gene="gspM"
                     /locus_tag="ACHY09_003713"
     CDS             9494..9955
                     /gene="gspM"
                     /locus_tag="ACHY09_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFS3886628.1"
                     /translation="MIKSWWAEKSTSEKQLVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEQITLKHLSFREQP"
     gene            9955..10632
                     /gene="gspO"
                     /locus_tag="ACHY09_003714"
     CDS             9955..10632
                     /gene="gspO"
                     /locus_tag="ACHY09_003714"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFS3886629.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10661..11137)
                     /gene="bfr"
                     /locus_tag="ACHY09_003715"
     CDS             complement(10661..11137)
                     /gene="bfr"
                     /locus_tag="ACHY09_003715"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFS3886630.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREES"
     gene            complement(11209..>11387)
                     /gene="bfd"
                     /locus_tag="ACHY09_003716"
     CDS             complement(11209..>11387)
                     /gene="bfd"
                     /locus_tag="ACHY09_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFS3886631.1"
                     /translation="NGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRAAREVM
                     EDELMQLPEFKESA"
BASE COUNT         2963 a         2927 c         2729 g         2768 t
ORIGIN      
        1 acgcttcccc ttccatctgg cgaatcataa taaagatgct gccatcaata tattgattat
       61 atttataagc atagggttga ttatttttaa tgtgaattat ttccatacaa ctatagttaa
      121 aaatggacag atcattaatc aaccaacaaa cgcatttcag tctgagttca gccttgccgc
      181 tttatggcgt aacgaaaatc atgctggggt aaaagatgca aatcccgtgg cagttaatca
      241 ggaaacacct aagctctcta tagcgcttaa tggcattgta ctcacatcga atgatgagac
      301 ttcttttgtc ttaattaatg aggggagcga acaaaagcga tactccctca acgaagccct
      361 tgagtcagcg ccgggaacgt ttattagaaa aataaataaa accagcgttg tgtttgaaac
      421 tcacgggcat tacgaaaaag taactctcca tccagggcta cctgacatca tcaagcagcc
      481 tgattcagaa agtcaaaatg tactcgctga ttatattatc gccacgccga tacgtgatgg
      541 agaacagata tacgggcttc gattgaaccc tcgtaagggc ctcaatgcat ttaccaccag
      601 cttgctacaa ccaggggata tcgccctcag gatcaataat ctttcgctga cccaccctga
      661 tgaggtttca caggcattaa gtttactgct aactcaacaa agtgctcagt ttacaattcg
      721 tcgcaacggc gtaccccgct tgataaatgt ttccgtcgcg gaacttacag gaatgaatgg
      781 acagcgtcat gaaaggactc aataaaatca cctgctgctt gctggcagca ctactcatgc
      841 cttgtgcagg gcacgctgag aacgaacaat acggcgctaa cttcaataac gccgatatcc
      901 gccagttcgt ggaaatagtg ggtcagcatc ttggcaaaac gatcctgatc gacccttcgg
      961 tacagggaac catttccgta cgcagtaatg atacgtttag ccaacaggag tactaccagt
     1021 tctttttaag tattcttgat ctttacggtt attccgtgat cacgctggac aatggttttc
     1081 tgaaagtggt gcgctcagct aatgtaaaaa catcgccagg gatgattgct gacagttctc
     1141 gtccaggcgt aggtgatgag ttggtcaccc gaatcgtacc gcttgagaac gttcctgctc
     1201 gtgacctggc tcccctgctc cgccagatga tggatgcggg tagcgtcggt aatgttgtgc
     1261 attatgaacc ctccaacgtt cttattctga ccggtcgtgc ctccaccatt aataaactga
     1321 ttgaagtcat aaagcgcgtt gatgtcatcg gcacagagaa gcagcaaatt attcatctgg
     1381 aatatgcgtc agcggaagat ctcgccgaaa ttttgaatca attaatcagc gaaagccacg
     1441 gtaaaagcca gatgcctgcc ctcctttccg cgaagattgt ggcggataag cgaaccaact
     1501 ctcttatcat cagcgggccg gaaaaagcac gccagcgcat cacttcatta ctgaaaagcc
     1561 ttgatgtaga agagagcgag gaaggaaata ctcgggttta ttacctgaaa tatgctaaag
     1621 ccacgaatct ggtggaagtg ctaaccggtg tttccgaaaa gctgaaagat gaaaaaggga
     1681 atgagcgtaa gccctcctct tctggcgcga tggataacgt cgccattacc gccgatgaac
     1741 agactaactc tctggtcatt accgctgacc agtccgtcca ggaaaaactc gccacggtaa
     1801 ttgcgcgtct ggacattcgc cgtgcacagg tgctggttga ggcaatcatc gttgaagttc
     1861 aggatggaaa tggacttaac ctcggcgtgc aatgggcgaa taaaaacgtt ggagcacagc
     1921 aatttaccaa taccggatta ccgattttta acgctgcgca aggtgtggct gattataaaa
     1981 agaatggtgg gatcaccagc gcgaatcctg cctgggatat gtttagcgcc tacaatggca
     2041 tggccgcagg ctttttcaat ggcgactggg gcgtattgtt gactgcgctg gccagtaaca
     2101 ataaaaatga catcctcgcc accccgagca tcgtaacgct ggataataaa ctcgcgtcct
     2161 tcaacgttgg tcaggatgtg ccggtgctat ctggctctca gaccacttct ggggataacg
     2221 tctttaatac tgtcgaacgc aaaacggtgg gcacaaaact caaagtcact ccgcaggtca
     2281 atgaaggcga cgcggtactg ctcgaaatag agcaggaggt ttccagcgtt gactcttcgt
     2341 ctaattcgac gctcggcccg acatttaaca cccgaactat tcaaaatgcc gtgctggtca
     2401 aaaccggtga aacggtggtc ctgggcggat tgctggatga tttttctaaa gagcaagtgt
     2461 caaaggttcc tctgcttggc gatattcctt tagtggggca actcttccgc tatacttcga
     2521 ccgagcgcgc taaacgcaac ctgatggtat ttatccgccc aacgattatc cgtgacgatg
     2581 atgtttatcg ctcactgtca aaagagaaat acacccgcta ccgtcaggag caacaacagc
     2641 gaatcgacgg gaaatcaaaa gcgctggttg gctcggaaga tttgccggtg ctggatgaga
     2701 acacgttcaa cagccatacc cctgcgccct cggcacggta aggcattctt atgagaattc
     2761 actcaccgta ccccgccagt tgggcgctgg cacaacgaat tggttatctc tattcagagg
     2821 gcgagattat ttatctcgcc gatacgccat tcgaacggtt actcgatatt caacgtcagg
     2881 ttggccagtg ccagaccatg accagcttgt cacaggctga ttttgaagcc cggctggaag
     2941 cggtgttcca tcagaatacc ggtgagtcgc aacagattgc gcaggatatc gatcaatccg
     3001 tcgatcttct ctcgctttcg gaagagatgc ccgcaaatga agatctcctg aatgaagatt
     3061 cagcggcacc ggttatccgc ttgatcaatg cgattttgag tgaggccatc aaagaaaccg
     3121 cctccgatat ccacattgaa acctatgaaa aaacaatgtc gatccgtttt cgcatcgacg
     3181 gtgttttgcg gacaatttta cagccaaaca aaaaactggc ggcactgctt atctcccgaa
     3241 ttaaggtcat ggctcgtctt gatatcgccg aaaaacgtat tccacaggat ggacgtatta
     3301 gtttgcgtat cgggcgacgt aacatagatg tccgcgtatc cacactgccg tccatctatg
     3361 gtgaacgcgc cgtgctccgt ctgctggata aaaacagcct ccagctttca ttgaacaacc
     3421 tggggatgac ggcagcggat aaacaggatt tagaaaatct catccagctt ccacacggta
     3481 ttatcctggt gaccggaccg acaggctccg gtaaaagcac cacgctctac gccatccttt
     3541 cggcgctgaa tactcccggc cgcaatattc tgacggtaga agatcccgtg gaatatgagc
     3601 tggaaggcat tgggcaaacg caggtgaata cccgtgtgga tatgtctttc gctcgcggcc
     3661 tgcgcgccat acttcgccag gacccggatg tcgtcatggt gggggaaatt cgtgatacag
     3721 aaaccgcgca gattgcggtt caggcctcgc tcaccggcca tctggtactc tcaacactcc
     3781 acactaacag tgcatcaggc gcagtgaccc ggctccgcga catgggcgtc gaatcattcc
     3841 tgctttcgtc ttccctggca gggattatcg cgcaacgtct ggttcgtcgc ctgtgtccgc
     3901 aatgccgaca attcacgccc gtatcacccc aacaagcgca gatgtttaaa tatcatcagc
     3961 tcgcggtgac aacaattggc actcccgtag gctgccctca ttgccatcaa tccggctatc
     4021 aggggcgcat ggcgatccac gaaatgatgg tggtgacgcc ggaattacgg gccgctattc
     4081 atgaaaatgt ggatgaacaa gcactggagc gactggtccg gcaacaacac aatgccttaa
     4141 tcaaaaatgg cctgcaaaaa gtgataagcg gtgacacctc ctgggatgag gttatgcgcg
     4201 tcgccagtgc cacgctggag aacgaagcat gaattatcgc tatcgcgcca tgacccagga
     4261 tggtcaaaaa ttgcagggga tcattgatgc taacgatgaa cgtcaggcac gactgcggct
     4321 gcgtgaagaa gggcttttcc tgctggatat tcgcccccaa aaaagttcgg gagtaaaaac
     4381 acgtcgcccg aggatcagcc atagtgaact gacgcttttc acccggcagt tggcaacctt
     4441 aagcgcagcg gcattacctc tggaagagag ccttgccgta atcggtcaac aaagcagtaa
     4501 taaacgactg gctgatgtgt taaatcaggt acgcagcgcc atccttgaag ggcatcccct
     4561 ttccgatgcg ttacaacatt ttcccacgct tttcgattcg ctctaccgta ccctggttaa
     4621 agcgggcgaa aagagcgggc tgctggcccc ggtgttggaa aaactggctg attacaatga
     4681 aaatcggcag aaaatccgca gcaaactcat tcagtcgctg atctacccct gcatgctgac
     4741 tgcggtggcg attgtggtcg tgattattct cctcactgct gtcgtgccca aaattaccga
     4801 acagttcgtg catatgaagc agcaactgcc gctgagtaca cgcattcttt taggtctgag
     4861 cgacacgttg caacataccg gcccgacatt actagcgaca gtgtttattg tcgctgtagg
     4921 tttctggctc tggttaaaac gcggcaataa ccgccaccgt tttcatgcca tgttgctgcg
     4981 cgttgcactc atcggcccgc tgatttgcgc cattaacagc gcacgctatc tccgcacttt
     5041 aagtattttg caatccagcg gcgtccctct gctggatggg atgaatttgt ccaccgaaag
     5101 tctcagcaac ctcgaaatcc gcaagcgtct ggcaaatgcg gcagaaaacg ttcggcaggg
     5161 taacagcatt catctttcgc tggaacaaac ggcaatcttc ccgccgatga tgctctacat
     5221 ggtggcctct ggcgaaaaaa gcgggcagct cggcacgtta atggtcagag cagcagataa
     5281 ccaggagaca ctccaacaaa atcggatcgc cttaacgctc tccatcttcg agccagcact
     5341 cattattacg atggcactga tcgtcctgtt tattgtcgtc tcggtactcc aacctcttct
     5401 tcaacttaac tcaatgatta attaaggaaa gtatatgcgc gcaacggata agcaacgcgg
     5461 ttttacatta ctggaaatta tggtggtgat cgtcatcatt ggcgtacttg ccagcctggt
     5521 ggttcccaac ctgatgggca ataaagaaaa ggcggataaa caaaaagccg tcagcgatat
     5581 tgtggcgctg gaaaacgccc tggatatgta caagctcgac aatcatcgct atccaaccac
     5641 aaatcaggga cttgaatcct tagtcgaagc gccgacactg ccaccgctgg ccgcaaacta
     5701 taacaaggaa ggttatatca agcgtctgcc tgccgatccc tggggcaatg attatgtcct
     5761 cgttaatcct ggtgaacatg gtgcatacga tctgctttca gcagggcccg atggtgaaat
     5821 gggaaccgag gacgacatca ccaactgggg tttgagcaag aagaaaaagt aagtcagcga
     5881 tgaatcagca acgcgggttt accctgctgg agatgatgct ggtgctggcg ctggtcgcta
     5941 tcacggcaag tgtggtgctc ttcacatatg gtcgggagga tgccgccaac actcgtgcgc
     6001 gcgaaacagc ggcgcggttt accgctgcac ttgaattagc catcgaccgc gctacgctca
     6061 gtggtcagcc tgtcggtatc catttctctg attcagcatg gcgcattatg gtaccgggca
     6121 aaacaccatc agcctggcgc tgggttccat tacaagaaga tgccgcagac gagagcaaaa
     6181 atgactggga tgaagaactc tcaattcatc ttcagccatt taaacctgat gacagcaacc
     6241 aaccgcaggt ggtgattctg gccgacggac aaatcacgcc cttctctttg ttgatggcta
     6301 acgcagggac cggtgagccg ctcctgacgt tagtctgttc aggatcatgg cccctggatc
     6361 aaacgctcgc cagagatacc cgcccatgaa caaacaatca gggatgacac tgcttgaagt
     6421 cttactggcg atgagtattt tcgccactgt tgcgctggct ttaatgagca gcatgcaagg
     6481 gcaacgaaat gccatcgagc ggatgcgtaa tgagacgctg gcgctgtgga tcgcagataa
     6541 ccagcttaag tcgcaagata cattcaacga tgaaaacacg tccagttcag gcaaagagat
     6601 aattaatggc gaagagtgga actggcgcag cgatgtccat tcaagcaaag acggcacgct
     6661 tctggaacgc accataacgg tgacgttacc caacggtcag aaaaccgctc tgactcgtta
     6721 tcagagcatc catgatagat ccgggcaggc gcaagatgat taatcgccaa cagggtttca
     6781 cattgctgga agtcatggcg gcactggcga tcttctcaat gcttggcgtg ctggcgttta
     6841 tggttttttc gcaagcgtct acgctacatc aacgctcaca gaaagaaatc cagcagttca
     6901 atcaactgca acgcaccatc accattcttg ataacgatct cctgcaactg gtcgctcgcc
     6961 gaaatcggag tacggataaa atcatggtgt tgggtgaaga ggctattttt accacgcaaa
     7021 gtcgcgatcc gcaggccccg ctcaatgaag cgcaaacctt acaaacggtt cactggtatc
     7081 tgcgaaatca tacgctttac cgtgctgttc gtacttctgt ggatggcagg aagaatcaac
     7141 ctgcccaggc gatgctggag catgtcgaga gctttcttct ggaaagtaac agcggggaaa
     7201 gccaggagct tcctctctca gtcacgttac atctgaagac tcagcaatat ggggcgttac
     7261 agcgccgttt tgcattacct gagcagttag cgagagaaga gtcgccagct cagacgcagg
     7321 ccgggaataa taaccatgaa taatgaacaa cgcggcgtag cactgctcat tgtactcatg
     7381 ttactggcgc tcatggctgc tctggctgca gacatgacga tcagttttca cggccagctt
     7441 catcgaaccc gccaggtaaa ccatcatttg caacggcaat atgatattga gctggcggag
     7501 aaacttgcgc ttgccagcct caccctggac gttaaagata acgacagaca aaccacgttg
     7561 cagcagttct ggccacaacc gcaacagttg cagctggaaa atggcaatac agtgaagtgg
     7621 cagttacgcg atgcccagca ctgttttaat ctcaatgcct tagcaaaaat ctctgacgcc
     7681 ccgctggctt cccctgattt ccccgcccag gtattcagtg ccctgctgat taacgccggt
     7741 atcgatcgcg gaaacaccga tgagatcgtg caatcaattg ctgactatat tgatgtcgat
     7801 gactcaccac gctttcacgg cgcagaagat aacttttacc aaagccagac gccgccccgg
     7861 catagtgcca atcagatgct ttttctgacc ggcgaattgc gtcagataaa aggcataacc
     7921 gaaaacatct atcagcggct tatcccgtat gtctgcgttc tcccaaccac ggaacttagc
     7981 atcaatctta atatgcttac ggagaacgat attccgctct tcagagcgtt attcctcaat
     8041 aacatcacgg atgctgacgc tcgggtctta ttacaaaaaa ggcccaggga aggctggcta
     8101 accacggatg catttcttta ctgggcgcag caggatttct ccggcgtgaa gcctctggta
     8161 gctcaggtga aagggcatct ctttccctat agccgctatt ttacgctgag tacggaaagt
     8221 atcagcgacg aacaatccca gggatggcaa agccatattt tcttcaaccg taaacagcag
     8281 agcgcccaaa tctatcggcg tacgctgcaa ctctactaat cataaggatc gtgatgcctg
     8341 aatcgttgat ggtcatacgt tcttcctcga cgctgcgtaa gcattgggaa tggatgacgt
     8401 tctccgcaga tagcgtatct tcggtacata cgcccacgga cgatcttccg ctggagtcgc
     8461 tggccgatca gcccggtgcc ggaaatgttc atcttttgat cccaccagag ggccttttat
     8521 atcgttccct cacgctgccc aacgccaaat acaaactcac cgcacaaaca ttgcaatggc
     8581 tggcagagga gacgttaccc gacaacaccc aggactggca ctggacggtt gtcgataaac
     8641 aaaacgaaag tgtcgaggtg ataggcattc agtcggagaa actcagtcgc tatcttgagc
     8701 gtttacatac tgccggactc aacgtgacgc gggttttgcc agatggctgt tatcttccct
     8761 gggaagtaga tagctggact ctggttaacc agcaaacaag ctggcttatt cgcagtgccg
     8821 cacatgcctt caatgaactg gatgaacact ggttacagca tcttgctgcc cagtttccgc
     8881 ctgagaatat gcgttgttat ggtgtggcac ctcacggcgt tgcagcgacc aatccactta
     8941 tccagcatcc tgaaatacca tcactgagtt tgtattctgc cgatattgca ttccagcgct
     9001 atgacatgct gcatggtgtt tttcgtaaac agaaaactgt aagcaaatcg ggaaaatggc
     9061 tcgcccgcct ggcagtaagc tgtctcgttc tcgccattct ttcttttgtt ggcagccggg
     9121 gcatcgcgct ctggcaaacg cttaagattg aagatcaact tcagcatcaa cagcaagaaa
     9181 cctggcaacg ttatttcccg cagatcaaac gcacccacaa ctttcgtttt tactttaagc
     9241 agcaactcgc tcagcaatat cctgaagctg tgccgttgct ctatcattta caaacacttc
     9301 tgctggaaca ccctgaactg cagttaatgg aggcgaacta tagccagaag caaaagtcgc
     9361 tgaccttaaa aatgagtgct aagagtgaag ccaatatcga tcgtttctgt gaattaacac
     9421 agtcctggct tccgatgcaa aaaacagaaa aagatccggt cagtggtgta tggacagtaa
     9481 ggagctcagg gaaatgatta aatcatggtg ggcagaaaaa tccacatcgg aaaaacagct
     9541 cgttgcggcg ctggcggttc tctcgctcgg cgttttttgt tggcttgggg tgataaaacc
     9601 gatagatacc tacattgcag agcatcagtc ccacgcgcaa aaaattaaga aggacatcaa
     9661 atggatgcag gatcaagcga gcacccatgg tttactcggt catcctgcgc ttacgcagcc
     9721 catcaaaaat atccttctcg aggaagcaaa acgtgagaat ttggctatca cgctggaaaa
     9781 cggtccggac aatactctga caattcatcc tgtcacagct cccctcgaaa acgtctcccg
     9841 ctggctcact acggcacaga ttacatacgg tattgttatt gaagaccttc agtttactct
     9901 tgccggaaac gaacagatca ccctcaagca tttgtccttc agggagcaac cataatgaca
     9961 atgctactgc cgctcttcat tctggttggt tttattgcag gttattttgt taatgccatg
    10021 gcctatcacc tctcgccgtt ggaggataaa acggcgttaa cgtttcgcca ggtattggtt
    10081 catttcaggc aaaaaaaata tgcctggcat gatacagtgc ccctgatact ttgcgttgct
    10141 gctgctatcg cctgcgccct ggcacctttc acgcccatcg tgactggcgc actctttctc
    10201 tatttctgtt tcgcactcac gctcagtgtt attgattttc gcactcagct cctgcccgac
    10261 aaactcacct taccgctgct ctggcttggc ttggtattta atgcgcagtc tggattaatt
    10321 gatttacatg atgcggttta cggcgcggta gctggctatg gggtgctgtg gtgtgtttac
    10381 tggggcgtct ggttagtttg tcacaaagag ggattgggct acggtgattt caagctactg
    10441 gccgctgcag gcgcgtggtg tggctggcaa acgttgccaa tgatactgct aattgcctca
    10501 ctgggtggca ttggttacgc catcgtttca caacttctgc aacgccggac tataaccact
    10561 atcgcattcg gcccgtggct tgcgctcggc agcatgataa acctggggta tctggcctgg
    10621 atctcgtatt aaccgggagg gttctccctc ccgacacggc tcaactttct tcgcggatct
    10681 gtgcttgcag ataattttgc aggcccatct tctgaatcag atcaagttcc gtttccagcc
    10741 agtcgatatg gccttcttca tcacgcaaaa tttctatcat catatcgcgg ctgacgtaat
    10801 catgaacgct atcggcataa ccaattgcct cacgcaaatt cttcgcgcca tccagctcaa
    10861 gtgccagatc agaacgcagc atttcctcaa catcctcacc aatgttcagt ttgcccaggt
    10921 cctgtaaatt tggaagacct tccagaaaaa gaatgcgctc aatataacga tcggcgtgtt
    10981 tcatctcatc aatggattca tgatactcca catcattgag acgtttgaga ccccagtttt
    11041 taaacatccg ggcatggaga aagtactgat tgattgcgac aagctcattt cccaacagtt
    11101 tgttgagata atttataacc ttagtatcac ctttcatttt ttgactcctt cgcttccact
    11161 ctttgaagcg tagaacggct tacgagtaag tcaaaaaaca cactccgttt atgcggactc
    11221 cttaaactcc ggcaactgca ttaattcatc ctccatcact tcacgcgcgg cccggacaca
    11281 tttaccgcac tgattgccga ccggaataaa tttttttaat tgctggaacg agtgagggga
    11341 aaactggcgc acagcctgac gaattttttt atcgctgata ccattac
//
LOCUS       DAZUCC010000163        11371 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZUCC010000163 DAZUCC010000000
VERSION     DAZUCC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.164"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..314
                     /locus_tag="ACHY09_003717"
     CDS             6..314
                     /locus_tag="ACHY09_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFS3886632.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            727..1707
                     /locus_tag="ACHY09_003718"
     CDS             727..1707
                     /locus_tag="ACHY09_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFS3886633.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGADIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            1700..2116
                     /locus_tag="ACHY09_003719"
     CDS             1700..2116
                     /locus_tag="ACHY09_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFS3886634.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(2118..3392)
                     /locus_tag="ACHY09_003720"
     CDS             complement(2118..3392)
                     /locus_tag="ACHY09_003720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS3886635.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAIEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(3392..3829)
                     /gene="umuD"
                     /locus_tag="ACHY09_003721"
     CDS             complement(3392..3829)
                     /gene="umuD"
                     /locus_tag="ACHY09_003721"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFS3886636.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLHPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(3826..4074)
                     /locus_tag="ACHY09_003722"
     CDS             complement(3826..4074)
                     /locus_tag="ACHY09_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS3886637.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSLSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            4492..5394
                     /locus_tag="ACHY09_003723"
     CDS             4492..5394
                     /locus_tag="ACHY09_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFS3886638.1"
                     /translation="MYFSAEPAQITEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIKVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            5391..5702
                     /locus_tag="ACHY09_003724"
     CDS             5391..5702
                     /locus_tag="ACHY09_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886639.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRT
                     R"
     gene            5778..5849
                     /locus_tag="ACHY09_003725"
                     /pseudo
     CDS             5778..5849
                     /locus_tag="ACHY09_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5931..7631)
                     /gene="ltrA"
                     /locus_tag="ACHY09_003726"
     CDS             complement(5931..7631)
                     /gene="ltrA"
                     /locus_tag="ACHY09_003726"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS3886640.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWVRYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKLLPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNIGNLAMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            8253..8864
                     /locus_tag="ACHY09_003727"
                     /pseudo
     CDS             8253..8864
                     /locus_tag="ACHY09_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            8865..9086
                     /locus_tag="ACHY09_003728"
     CDS             8865..9086
                     /locus_tag="ACHY09_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886641.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            9046..9532
                     /locus_tag="ACHY09_003729"
                     /pseudo
     CDS             9046..9532
                     /locus_tag="ACHY09_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            9577..10347
                     /locus_tag="ACHY09_003730"
     CDS             9577..10347
                     /locus_tag="ACHY09_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886642.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDVSRLATLLISRHDGVLPPVLASAMQKLTGTVAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            10761..11186
                     /locus_tag="ACHY09_003731"
     CDS             10761..11186
                     /locus_tag="ACHY09_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS3886643.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            11233..>11371
                     /locus_tag="ACHY09_003732"
     CDS             11233..>11371
                     /locus_tag="ACHY09_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886644.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DE"
BASE COUNT         2491 a         2890 c         3027 g         2963 t
ORIGIN      
        1 atctcatgag caaagcgaaa tttagtgcac aggctgaaaa actgaaaaac ctggtggatt
       61 ctgccagatt acaaacttct tcttctgccg taacagcaac agccccgtct cagccaaatg
      121 aaggtgcaga ggaagtttca ggtcgtcccg tggctgccag gacgaaggct aagaatctta
      181 aggccatacc gctgtcgtat tttgaagctc atgcggaact taaagcgacc agtaaaacat
      241 ctctggattt tagctcatat attattgagg ctataagaga gaagcttgaa cgtgatggtg
      301 caataggtca ataaactatc ggatacctta agggtatctg atgtgtgtct tttaaatgcg
      361 ttatggggat tcttcacgta cctaaagagt ccccattaga catctttctg tttatggtga
      421 catgattcgt caccgctttc ctttcctctc agtttcacca tttcattcct ttcccttaac
      481 ctgaaccatg atgtccacga tgtttttata ttgcatttat gtatcacata taaccttcat
      541 aatgtttttg tatctatgat ttcctctggt cttaaagttg tgcgtacaat gtacatgcaa
      601 tttatcgtgt ttttatgttg catttgtcct ttcagaaaga ccgttttttc attataattg
      661 aatatacatt gcatgtatga agcatgtttg ttttttctat ttgtttatga gcaacggaga
      721 ctggttatga aaatctttat tgatgatggt tctaccaaca ttaaacttgc ctggttggag
      781 gatggtgacg tgaaaacgtt aatcagccct aacagcttta aaccggaatg gtcttttagt
      841 ttactggatg atgctgctcc ggctaattac gagatcgacg gcgagaagtt ttcatttgat
      901 ccattaagtg ctgatgctgt cgtaacgact gaaacacgct atcagtacag tgatgtgaat
      961 gttgtggcca ttcagcatgc gctacagcag accggcctga aagcgcagcc tgttgatgtc
     1021 atcgtcacat tgccgatctc tgaatacctt gacgctaaca atcagaaaaa taagcaaaac
     1081 attgaaagaa agaaaaaaaa tgtgatgcgt gaagtacggg tgcaggggag tgatgcattt
     1141 gttattcgca gtgtttccgt tcttcctgaa agtattcccg ctggtttcag tgttcttgcg
     1201 gggcttgagg atgatgaatc ccttttgatc gtagatctcg gtggtacaac tcttgatgtg
     1261 tcgcatgttc gtagcaaaat gacagggatt acaaaaacct ggtgtgatcc gaatatcggc
     1321 gtgtccctga ttacatccgg ggttaaggaa cagatggctg tacatgctaa tacaagagtg
     1381 agctcattcc aggctgacaa tatcattgtg caccgtaatg aacctgacta cctttcccgt
     1441 cgcatttata atgctgaaca gcgtgagtct atcattaatg ttatcaatga acggcagaag
     1501 ttgcttatta aacgagttaa tgatgtcatc agccgtttca ccgactatac acatgtaatg
     1561 tgtgttggtg gtggggctga cattgtggcc gaggctgtga agaacctgac taaggttcct
     1621 gatgagcgct tttatctgag ctcatccccg cagtttgatt tagtcatggg aatgataaaa
     1681 atgaaaggtg gtgtgactaa tgagtgatga aaataaatcc agacgctgct catttgaact
     1741 tttcccggat gaacggacag gagataagat agcagatgag ctcattgcaa atgaaaaact
     1801 gaaggagcgg ggacgtttca tgcgcgcgat gttagttact ggtgcggcgt ttgcagctat
     1861 agataaacgt cttcctttgc tgatatcgga gttactgaca gaaaatacga ctctggatga
     1921 tattaataag gtgatttcca gcgtaatacc aggtgctttt tcggttgaaa agaaattgct
     1981 tgaattactg gaaaaacaat ctggtctcca tacgtctgtg gattgcagta ctcctcttac
     2041 agagcagagt ctgtcgagaa atgacggtga agaccagacc agaagaaacg ctgaaaatat
     2101 gttcggagat gactgaacta acccagcctg gcaaggggaa tatctcgcca tttagttgta
     2161 taacactgac tcaacatttc ccgtttcatt tgccattcag gggcggttcc tcgccccgca
     2221 aaccacactt tcccttttcc cgactggttc agttcatcga gtgttttcat taacttttca
     2281 ctgtttttac ggggctggat ttcatcaaat aatcccggct gcgctatacc cgatggtgtg
     2341 aaatcagcca gcatgactcc tgccttcata tagcggtacc cttcacgcca gacatggttt
     2401 aaggctctgc atgcggcggc aataatgtcc cggctgtcct gtgtgggcaa tggaagcttt
     2461 tccacagcgg cattgctgta acagggttct tttactgcaa agggggatgt ccgtacaaat
     2521 gtcgtcacct gccggcaata ctgacgctcc ccacgtagtt tctctgcggc ccgctctgca
     2581 tactgaacaa cagcctggtg catggcatct ttgtctgtga ttcgttcacc aaaactgcga
     2641 ctacagacaa tctgctgttt tgccggtggt gcttcttcca gggatatgca ggactcgccg
     2701 ttgagttcgc gtaccgtacg ctcaagaatg acgctgaagt ttttccggat gaatgccgtg
     2761 ttagcctgcg ccagctgcag tgctgtgtta atacccagcg cattcagctt ttccgtcagt
     2821 ctgcgtccta ctccccagac ctcaccaact ggctgcagcc ccagtagctt caaggtccga
     2881 ttacggtttt ctgccgtcag cgcgaccact ccggaaaact gtggccattg ctttgttgcc
     2941 cactgtgcac ttttagccag cgtttttgta ggcgcaatgc ccacccccat ggtgagtcct
     3001 gtccagctct ttacctgttc cctgagctga tgaccaaaaa actccggaga gatgcaatga
     3061 tttatccccc gcaaatcaat aaacatttca tcaattgagt agggctcaat tgcgggagaa
     3121 agcgactcca gaacagccat gacccgttgg ctcatgctgt ggtacagcgc ataattgctg
     3181 gaaaatacat gtattttctt ctccaggcgc atctgtctca cctgaaacca gggctgcccc
     3241 attctgatgc caagggcttt tgcctccggg ctgcgcgcga tcacacagcc atcgttattg
     3301 ctgagtacga tgaccggttc gttgcgaagg tccgggcgga aaactttttc acatgaggcg
     3361 tagaaactgt tgatatcagc cagtgcaaac atcagcgtaa ctcccgcgtc ctgtgtatca
     3421 cgtgagtgac aacaccaaaa atacagatgt tttccggata cagtgtgcgg aactccgggc
     3481 tgtctgaaac cggctccagt gccgggcgtg ggtgcaacag cagtcgtttg acggtgaact
     3541 ccccgtcgat ctcagcgata acgatgtccc cgtgttgtgg tttttcggcc ctgtccacta
     3601 ccagcagatc accattctgc acgccagcct ggttcatcga ttcaccgctg gcgcgcagaa
     3661 agaaggtggc tgcaggtctg ctgatgcaat agctgttcag atccagttcc tgctcagcat
     3721 aatcagtggc aggagaagga aaaccggcct ggcaacgatc ggcaaacaac ggtcgcacgt
     3781 aactgtcatc gcctgaaggg gcagccggac ggtgataaac ggtactcatg ctgcaggcag
     3841 ccagctgtcg tcctgccaga tttcttccag cagagacagg atacgttctt tatccttgtc
     3901 gttgcgggct cctgtgacag atagagacgg ggcgctgccc tggtctatac gcagccagat
     3961 ttccgggtag tgtggtttca gacgttgttc gatttcattc tgtagcgcct gaagtgtgcc
     4021 tgatttgagg ttttttgtgc tctggcggtc gaaaagaatt tcaatgcgga tcatgttctc
     4081 atctcccttt tcttttgctg tatatgtata cagtattcta ataactgaat ccgcacacag
     4141 tcaaatgtca ttgttctgac taaaaagcga tcaattctct gagggggtgg ctgtatgcct
     4201 gctgtggagt gctgtatcca gcttatccac agcattttgt gcacggttct gtggacaaaa
     4261 tacctggtta cccaggccgt gccggcacgt taaccgggct gcatccgatg caagtgtgtc
     4321 gctgtcgccg gcctcctcac ccggtcacgt ttcgtcgttc ctcctccacg cgctgcggct
     4381 tcggggccgc acctgcattc gtatgcggtc gcccggttac aggtgcggca cggcctgatg
     4441 gaggccgcat gtgagaggag aattcctatg ccaaactggt gctcgaatcg tatgtatttt
     4501 tctgccgaac cggcacagat tactgagatt aaacgactgg ccagcggtgc agtcacaccg
     4561 ctttatcgcc gcgccacaaa tgaaggtatt cagctgtttc tggccggaag tgccggactt
     4621 ctgcagacca ctgaagatgt gcagtttgaa ccgtgccccg gactgacggc tgccggacgt
     4681 ggtgttgtat cgccggagaa tatcgcgttc acccgctggc tgacacacct gcaggacggt
     4741 gtactgctgg atgagcaaaa ctgcctgatg ctgcatgaac tctggctgca gagcggtact
     4801 ggacagcgtc gctgggaagg attaccggac gatgtcaggg ataccatcac cgcacttttc
     4861 accgcaaaaa gaggtgactg gtgtggcttc tggagtaacg aggatgtatc ggtgtggtgg
     4921 aaccgtctgt gtgacaacgt actgccggaa aaaaccatgc cgtttgacct gctgacggtt
     4981 ctgccgaccc gcctggatgt tgaagtgaat ggctttaacg gtggtgttct gaacggtgtt
     5041 ccttctgcat atcactggta tacggaacag tatggcgtga agtggccttg tgggtatgag
     5101 gtgaatatca gcagtcaggg agacaacttc attaaggtgg atttcgacac gccgtggtgt
     5161 cagccggaaa gcgacgttat tgcagaatta agccgccgtt tcagctgcac gctggagcac
     5221 tggtatgccg aacagggctg tgatttctgt ggctggcagt tgtatgagcg cggagagctc
     5281 gttgatgtgc tgtgggggga actggaatgg tcttccccga cagatgacga tgagctgccg
     5341 gaagtcaccg gacctgcgtg gatagtcgac aaggtggcgc attatggcgg atgaagtatg
     5401 acggagacgg gcgggcaacc gcccgtttct tttccggtaa aggatgtcgc cgtgctcctc
     5461 tttttactgc gccggctgac gcggcgcggc ataaacgctg cctgtggtca gtgtcccgcg
     5521 tccggcgggc acgggacggg cgcttttacc gctcccggct ggcgtcggtg acagtgtacg
     5581 ccagcccgtc gcccttttct gatgaacgcc cttcaagccg ctttcgcggc ataaccttgc
     5641 cgtcaaaaag acgacggctg cggtattcca cggtcggcct gacccgttac cggacgcggt
     5701 gaacagccca caggcagcgg ggaacgggca ccacaggggt gccctcccgg agccctctgt
     5761 aagagaagga gttttgtatg tcccgttttg tccttggtaa ctgcattgat gttatggccc
     5821 gtattcctga taacgccatt gatttcatgt cgggtagcag ctgcacatta ctgtgcagcc
     5881 gccccctaag aaccgtacgt gccagtttcc cggcatacgg ctcaagcctt tcaataactc
     5941 cacattagag ccggcttcat aacagatatt ccctgatgtt ttgcagtgtg atgacatgcc
     6001 ggatgtaaca tggccagatt acctatgtta tccagtcccc cttcggattt cggcacaatg
     6061 tgtcgaagtt gccagcctgt tgtggcgttc actggcaggt ggcagacggg gcactgacct
     6121 ccctgtcgga gccacagatt tcttaggcga gagtaaccct gtatggattc cagcatcgta
     6181 gcttttagcc gtgcttcaaa gtacgattcg tagctgggat caaaagggtt agcagtgctt
     6241 tttatttgtc tgtgtctttt gactggcata tccgagattt catatagcgc cagtaactca
     6301 cgtttgcctg atggcaggag cttccctgtt tctgtgccga atacccagtg tcggaagtta
     6361 actgaacgaa agtatttctt tcttatccag cctgtacctt tctttgggtg tcgtcgacat
     6421 gcccagcgcc atagtgcttt ccatatttca tgactgactt tctgaaagat cttttttgaa
     6481 acaacatgac gatagtatct gacccagcct ttgaggactg ggttgagatg ccatatcagt
     6541 gtgctctggc tgactgtcgg attgtctttt atcgtctggc ggaccctttt caggaattcc
     6601 tttacctttt ggggtgaagg tttaatcaac agtttcccgt tatatttgcg gatgttcata
     6661 cccagaaagt cgaatccatc actgatgtgt gtgattcgtg tcttttctgg tgagagggta
     6721 agccctcttt ctgccagaaa gttttctaac aagggaagaa ccttttctga cagcgtgtcg
     6781 tggcttatcc ccgtgatcac aaaatcgtcc gcataacgga ttatgttgat cttttctgga
     6841 ttcagtccgc ctcgatagaa ggttttcttc aacagaggct gcagaccatc cagaaccatg
     6901 tttgccagcg ttggagatat tattccgccc tgtggagttc ctgcactggt tgggtataat
     6961 ttccccaaat caatatatcc ggctttcagc catcgtttca ggatcgccgt atccgttggg
     7021 atattggcga ttagccattc atgtgaaatc tcatcaaaac aactgcgaat gtcggcttcc
     7081 agaatccatt gtgcccgatc agccctgtgc aacgttgtga aacattgctc aatggcatcg
     7141 gctgttgagc gacagggcct gaacccaaac gaatttgggt cagccgttgt ttctgcaaca
     7201 ggttccagcg ccatcagata gagtgcctgc atggctctgt ctttcattgt cgggatccct
     7261 aatggtcgct ttttgccgtt agattttggg atatatatac gccgtagcgg aagaggtttg
     7321 taaccccttc ttttcagcgt gtatatagcc ttcatcttgc tgccgggttt tgaccatgtt
     7381 ttgccatcaa cacccggcgt tctcttccct ctgttatctg tcactcgttt aactgccatt
     7441 gctctggcgc tgaacgagtg ggtgagtagc cattgcaagg cttttacctt gcccgctttc
     7501 tcctcctgta ttgccttcac gatacgcgcc tgcagccttc tgacctcgcg acggcacttg
     7561 gcccagttga tattgtgcca gtgagtcacg atgtcggtgg gtgcacccgc gtttatgttt
     7621 ttcgcagtca tctgcttacc ctccttcaaa ggtattcagg ttatctcgtg ttgaaagacc
     7681 atgcggaagt ctgcccgctt tcgcgtggaa tgatgttgct gaaacagctc aactcctgtc
     7741 catcccatta caggatggca ttcgctttct ccgcactcct gtacccgcat cactgacagc
     7801 tttcctcgcg gttagcctgc cctgagggca gtgatacggg cttaccgtgt tccgcacagt
     7861 ttgcagggat gggttaggtt ctgcctatcc accggtagct ttgatgtccg tgcctcccca
     7921 gtcaaaagag gaaggtccag ctacgttccc attttgggtc gggcctgtca gtgcctttgg
     7981 cccgttcagg gttgacgatg tttatcagca gttcacatgt gttaaccctg ccatccagcc
     8041 ttgcgcccca tccgccctga cactggcaga catgcttcct cctcgcggat tccacatggt
     8101 gtgaaaaccg ggctacgttg tcccgaaggc ttcataccga acagttaccc gctctgcatg
     8161 cttcggtagg ctacaggcga cggaacgcct ggttttacta cttcctttac atgaagttaa
     8221 aacaacaaac tgtgcgactt tcacgtcgca cccttaccga tcctccatat ctcgtcggtt
     8281 tccgtgaccg tcaggggcgc accattgccg gtgacgttaa tgatgactgg ctgcaacctg
     8341 ccagcaatga aatgtaccgc gtactcaaaa aagatgcgct gatggtgagt ttctatggct
     8401 ggaaccgtat tgaccgcttt atggcagcct ggaaacgcgc cggattcagc gtggcagggc
     8461 accttgtgtt caccaaaaac tacacatcca aatctgcgta tgtcgcgtac cgccacgaat
     8521 gcgcttacat cctggcaaag ggcagtccgg cccggccccg taacccgctg ccggatgtgc
     8581 tgggctggaa atattcgggc aaccgccatc acccgacgga aaagcccgtt accagcctgc
     8641 aaccactgat tgagagcttc acacatccga acgcgattgt gctggacccg tttgcaggca
     8701 gcggctcaac ctgcgtcgcc gccctccagt ccggacgcag gtatatcggt atcgagctgc
     8761 ttgagcagta tcaccgtgcc ggacagcaac gtctggctgc cgtacagcgg gccatgcagc
     8821 agggggccgc aaatgatgac tggtttatgc cggaggctgc gtaaatgaac tatgccggac
     8881 atgaaaaact tcgcgccgaa gtggcggagg tggccaatgc catgtgcgac ctgcgtacaa
     8941 ccatgaatga gatggagcgg cggtacagct ttaatgctga caccctgccg gaacgtctgg
     9001 tgcgtcagac gttgtttcgc gcaaaccgcc tcctgatgga ggcatatacc gaaattctgg
     9061 aactggattc ctgcttcaaa gattgagggg agagagtatg tacggaacat gcgaaatact
     9121 ctgccgggag ctggcagcaa agtatccggg agacacgccg ctgatgctgg ttatctggtc
     9181 tccggaagag attcaggccc ttgctgacgg aatggatatt tccctgtccg atcatgaaat
     9241 caggaccgtc ctggcgcgcc tggaggacat cccggaagac cagcggactg agtccggtat
     9301 ttcttcgggt gcggctatgg agattatcag caacgtgagt gaaaaccgcc aggtgaccgt
     9361 ccctgctgaa ctgctggcgt ccctgattca gaccgctgaa caggcattgt ggaagcgtga
     9421 atgggccgcc cgtgataacg gcctcgtcgt cccggaatgc gtcacccgcc gtcaggcggt
     9481 ggttaatcag gcccgcgccc tgctgaaaaa caacacacac gaaaacgact gatgttatcg
     9541 ccgctttcgg gcggcgattc agggagacca ttaatcatga acaaaacgct gaatgcactg
     9601 gtttgtcgcc acgcccgtaa cctgctgctg gcgcagggct ggccggaaga gacggatatt
     9661 gaacaactga atccgcacta tccgggctgg atcagcattt atgtgcgact ggatgtatcc
     9721 cggctggcga cgttacttat cagccgccat gatggcgtgc tgccgccggt cctggcgtct
     9781 gccatgcaaa aactgaccgg aaccgtggcg gaacttgtac tgtccggcag ccagtggcag
     9841 gcgctgccgg tacttccggc agacggaacg caggtgtctt tcccgtatgc cggtgaatgg
     9901 ctgacggagg aagaaatccg ggcagttctt gatgcggtgc gcaatgcagt acgaagcatc
     9961 tgttaccagg tggcagacga tgcgcggcgt atccgggcgg cgctgaccac caccggtcag
    10021 acgttgctga cccgccagac gcgccgcttt cgtctggttg tgaaggaaag cgattacccc
    10081 tgctggcttg atgaagatga cgaaaatctg cccgtggtgc tcgatgccat tctgaaccgg
    10141 ggcgcccgtt tttcctctgt ggaaatgtac ctggtctgtg agtgtgtgga gcatatcctg
    10201 gccagtggtc tggtctgtga tgtgctgcgt ataccggatg aaccgtcccg tcgctggttt
    10261 gacagggata ttctgcggga ggttgttctg gaggcacggg atgaaatccg cagcatggca
    10321 gatgcactgg cgaaaatccg gaaatgatga cgctggcggg gaaaaatctc cgccttttcc
    10381 tgccggtccc cggacagagc aaaaggatgt cgccgcaggc tccgctttac ccggccatgc
    10441 ggggcatggc cttgtgggtt ttcagttatg tggcctcagc gtcgtgtgcg ggctgtgccg
    10501 tgcctccatc ttagccgggc tggcggggat gcaagggtaa aatgcacgcg gcggtcaccc
    10561 ggtccctcct tcccgtctgc cgtgattttc cggtcgttca cccgctcaga tttcgcggtc
    10621 tcaccctcca actcccgtca gccgtgtgcg tcaggctgcg gcttcccttg cgcccttgca
    10681 tccccgcctt atcgcccggc ttttctggag gcacggcacc gcccggtgtc gcggaatatg
    10741 tgaacaacgg aggaaatccc atgcagtatg cgaaacctgt cactctgaac gttgaagagt
    10801 gcgaccgcct ttcctttctg ccttacctgt ttggcaacga ttttctgtat gccgaggcgt
    10861 atgtgtacgc gctggcgcaa aaaatgatgc cggaatatca gggaggcttc tggcatttca
    10921 tccgcctgcc ggatggtggc ggttacatga tgccggatgg cgaccgtttt catctggtca
    10981 acggtgaaaa ctggtttgac cgcactgtga gtgctgatgc cgcaggtatc atcctgactt
    11041 cccttgtgat taaccgccag ttgtggctgt accacgacag cggggatgcg ggactgacac
    11101 acctttaccg gatgcgtgat gcgcagttgt ggagccacat tgaatttcat cccgaatgta
    11161 acgcgattta cgcagcactg gactgattaa cggacggggc ggcgatgccg cccctgtaaa
    11221 ggagacgaga acatgtactg tactgttaaa gaaattatcc gcgaagtgct gaatacagac
    11281 gtgccggaca gtgaatgtgt ttttgccgtg gtgctgaccc gcggggatgt gcgccacata
    11341 gcccgggact ggaacctgtc agacgatgag c
//
LOCUS       DAZUCC010000164        11238 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZUCC010000164 DAZUCC010000000
VERSION     DAZUCC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.165"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            170..1741
                     /gene="garD"
                     /locus_tag="ACHY09_003733"
     CDS             170..1741
                     /gene="garD"
                     /locus_tag="ACHY09_003733"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFS3886645.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQGIPLENASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAIP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1890..2225
                     /gene="prlF"
                     /locus_tag="ACHY09_003734"
     CDS             1890..2225
                     /gene="prlF"
                     /locus_tag="ACHY09_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFS3886646.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2225..2689
                     /gene="yhaV"
                     /locus_tag="ACHY09_003735"
     CDS             2225..2689
                     /gene="yhaV"
                     /locus_tag="ACHY09_003735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFS3886647.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2744..3553)
                     /gene="agaR"
                     /locus_tag="ACHY09_003736"
     CDS             complement(2744..3553)
                     /gene="agaR"
                     /locus_tag="ACHY09_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFS3886648.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3802..5082
                     /gene="kbaZ"
                     /locus_tag="ACHY09_003737"
     CDS             3802..5082
                     /gene="kbaZ"
                     /locus_tag="ACHY09_003737"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFS3886649.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCL
                     EHFGEADLVYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5105..5578
                     /gene="agaV"
                     /locus_tag="ACHY09_003738"
     CDS             5105..5578
                     /gene="agaV"
                     /locus_tag="ACHY09_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFS3886650.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5589..6368
                     /gene="agaW"
                     /locus_tag="ACHY09_003739"
     CDS             5589..6368
                     /gene="agaW"
                     /locus_tag="ACHY09_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFS3886651.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6358..7236
                     /gene="agaE"
                     /locus_tag="ACHY09_003740"
     CDS             6358..7236
                     /gene="agaE"
                     /locus_tag="ACHY09_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFS3886652.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7254..7688
                     /gene="agaF"
                     /locus_tag="ACHY09_003741"
     CDS             7254..7688
                     /gene="agaF"
                     /locus_tag="ACHY09_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFS3886653.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKLGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7685..8818
                     /gene="nagA"
                     /locus_tag="ACHY09_003742"
     CDS             7685..8818
                     /gene="nagA"
                     /locus_tag="ACHY09_003742"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS3886654.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAEIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LERIAQRCQRGGPGAQILGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHAQQIWIQGQLASF"
     gene            9168..10322
                     /locus_tag="ACHY09_003743"
     CDS             9168..10322
                     /locus_tag="ACHY09_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFS3886655.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10335..11195
                     /gene="kbaY"
                     /locus_tag="ACHY09_003744"
     CDS             10335..11195
                     /gene="kbaY"
                     /locus_tag="ACHY09_003744"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFS3886656.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2677 a         2913 c         2991 g         2657 t
ORIGIN      
        1 gatttgcgct ttaccttgcc gggcggcact gcaatccctg aaatgattga cattgatcac
       61 atttctgcat ttaaactcct gacattctta tttcacccaa tgaagttatt tatttttaaa
      121 tgagaccagg tcctcatttt aataacccct ggctggagaa tattgcacaa tggccaacat
      181 cgaaatcaga caagaaacgc caactgcgtt ttatataaaa gttcacgaca cagataatgt
      241 ggcaattatt gttaatgata atggcctgaa agcaggaacg cgttttccgg atgggctgga
      301 attaattgaa catattcccc aggggcataa agtcgcattg ctggacattc cggctaatgg
      361 tgaaattatt cgttatggcg aagtgattgg ttacgccgtg cgcgcaatcc cacgcggaag
      421 ctggatcgac gaatcaatgg ttgtactacc ggaagcgccg ccgttacaca cgctgccact
      481 ggcaaccaaa gtcccggcac ccttaccgcc actggaagga tacacctttg agggctatcg
      541 caatgccgat ggcagcgtgg gcaccaaaaa cctgctcggc atcaccacca gcgtccactg
      601 tgtggcaggc gtggtggact atgtagtaaa aatcattgaa cgcgatctgc tgccgaaata
      661 cccgaacgtc gatggcgtgg tggggctgaa tcatctgtac ggttgtggcg tggcgattaa
      721 cgctccggcg gcggtggtgc ccattcggac cattcacaac atttcgctta acccaaactt
      781 tggcggcgag gtgatggtga ttggcctggg ttgcgagaaa ttacagccag aacgcctgct
      841 ggtcggcacg gatgatgtac aaggtattcc gctggagaac gccagtattg tcagcttgca
      901 ggatgaaaag catgtcggtt ttcagtccat ggtcgaggat attttgcagg tcgccgaacg
      961 ccatttgcac aaactgaatc aacggcagcg agaaacctgt ccagcttcag aactggttgt
     1021 cggcatgcag tgcggtggca gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg
     1081 gtatgcgtct gatctactgg tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga
     1141 agtgcgtgac gcgatccatc tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg
     1201 gctgctggag gagatggagt ggtacgataa ctatctcaat atgggaaaaa ccgaccgtag
     1261 cgccaaccct tcgccgggca acaagaaagg cggtctggca aatgtggtgg agaaagcgct
     1321 cggctccatt gctaaatcgg gtaaaagcgc aattgttgaa gtgctgtcgc ccggtcaacg
     1381 cccgactaaa cgcggattaa tttacgccgc gacgccagcc agcgattttg tctgtggcac
     1441 acaacaggtg gcttcaggga ttaccgtgca agtgtttacg accggtcgcg gtacgccgta
     1501 cggcctgatg gcgatacccg tcattaaaat ggcgacccgc accgagctgg cgaaccgctg
     1561 gtttgattta atggatatta acgcgggcac tatcgctacc ggcgaagaaa ccattgaaga
     1621 agtgggctgg aagttgttcc actttattct cgacgtcgcc agcgggaaga agaaaacttt
     1681 ctcggatcaa tggggactgc ataaccagct ggcggtgttt aacccggcac cagtgacctg
     1741 atttcttttg gaacgtccct cgcaaaacat ggccttagtg ccatgttttt attgtttaaa
     1801 gtccacacat ccattaataa tgcatttgca ttaccttagt tcaagcttat aatttgagca
     1861 gaaaacaggc tgtaaaagga cagtaaataa tgcccgctaa tgctcgctct aacgctgtac
     1921 tgaccactga atcaaaggtc actatacgcg gacaaacaac tatccccgcg ccagtgcgtg
     1981 aggccttaaa actgaagcca ggcctggaca gcattcatta cgaaattctg cctggtgggc
     2041 aagtatttat gtgccgactg ggagatgaac aggaggatca tactatgaat gcatttttgc
     2101 gttttctgga tgcagatatc cagaacaacc cgcaaaaaac tcgtccattc gacattcaac
     2161 aaggaaagaa acttgtcgct ggcatggacg tcaacattga cgatgagatt ggcgacgacg
     2221 aataatggat tttccacaaa gggttaatgg ttgggcgcta tatgctcatc cctgttttca
     2281 ggaaacctac gatgctttag ttgccgaagt cgaggcatta aagggaaaag atcctgaaaa
     2341 ttatcagaga aaagctgcca caaagttatt ggcggtggtc cataaagtga ttgaggagca
     2401 tatcacggtc aatccatcat caccggcatt ccgtcatggc aagtcgttag gctctgggaa
     2461 aaataaagac tggtcacggg taaaatttgg tgctggtcgc tatcgtctct tctttcgtta
     2521 cagtgaaaaa gagaaagtca tcattctggg atggatgaac gatgaaaaca cgctgcgcac
     2581 ctacggtaaa aaaacagatg cctataccgt attcagtaaa atgttaaaaa gaggacatcc
     2641 tcctgccgac tgggaaagcc tcacccaaga gacagaagaa aaccattgat ggtgttcaca
     2701 atgcccgccg aacgcattgg tgttgaccag cgggcatcct ttctcattcc ccgaccagaa
     2761 tcacttcaac cccagccttt ctcagtcctt ccagactatc cgcaggaatg ccttcatcaa
     2821 caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactagaa cgattgaact
     2881 tactggaatc ggtgaccacg atgatccgtt ccgcaacttc gcacatccga cggtttaaac
     2941 gggcttcatc ttcattatgc gtgctgacgc cgcgctccag atcgatcgca tctacaccaa
     3001 gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatca ccgtaaaaag
     3061 attgcgactg acggcgcaaa tgccccccgg tcatcagcag ctcaacgcct tccgcttcca
     3121 gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcggtg tgcttgcgca
     3181 tcagacgagc aatctcaaaa gtggtggtcc cggagtcgag gatcacccga tgaccaggct
     3241 gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc agcgcgcttt
     3301 tatcttccac tgatggctcg actgacggcg ttgtgctatc gcagatcaac gcgccaccat
     3361 aggcacgcac agcgatcccc tgcttttcca gaaacgccag atcgttgcgg atcgtcacag
     3421 tagatacacc atacaacgcc gatagatcat taacttgcac acttccttgc tggcgcaggc
     3481 gttgaatgat ctgctctcgt cgctcgctgg tgcctgtcac tcgcttctca cctgaagcgt
     3541 cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt ttgcctatta
     3601 acgcctttct attaagcaaa tgcaagccca ccttacccat tggcgcaagc tactctcgtt
     3661 tcagtggctt tcattatgtt tcttttgtga atcagatcag aaaaccatta tctttcgttt
     3721 tatttttatc tcaccatgac gcagtatcaa ctgaaacaaa acgaaagatt aatgtcgcag
     3781 taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca gcacaaagcg
     3841 ggcaaaacaa atgggattta tgccgtttgt tccgcacatc cgctggtgct ggaagctgca
     3901 atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc caatcaggtg
     3961 gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt tgtttgtcag
     4021 ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggtga ccatctgggg
     4081 ccaaaccgct ggcaaaacct gcctgccgct caggcaatgg ccaatgccga tgatttgatt
     4141 aaaagctacg tggcggcagg attcaaaaaa attcaccttg attgcagcat gtcctgtcag
     4201 gacgatccga ttcccttaac cgatggcatc gtggccgaac gcgccgcccg tctggcgaaa
     4261 gtggcggaag aaacctgtct tgaacacttt ggcgaagccg atctggtgta tgtcattggc
     4321 accgaagtac cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc ggtcaccacg
     4381 ccggatgccg cccgcgccac gctggaagcc catcgtcacg cctttgaaaa gcaaggtctg
     4441 agtgccatct ggccacgcat cattgctctg gtggtacaac ccggcgtcga attcgatcac
     4501 accaacgtta ttgattatca gcccgtcaaa gcgaccgcct taagccagat ggtcgaaaac
     4561 tacgaaacgc tgattttcga agcgcactct accgattacc aaacgccgca atcgttgcgc
     4621 cagctagtga ttgaccactt tgccatcctg aaagttggcc cggcgctgac cttcgccctg
     4681 cgtgaagctc tgttctctct ggcggcgatt gaagaagaac tggtaccagc aaaagcctgt
     4741 tctggtctgc gtcaggtgct ggaaaacgtg atgctcgacc gcccggaata ctggcaaagc
     4801 cactaccacg gtgacggcaa cgcgcgtcgt ctggcgcgtg gttatagcta ctcggatcgc
     4861 gtgcgctatt actggccgga cagccagatt gatgacgctt tcgctcatct ggttcgtaat
     4921 ctggcggatt caccaattcc gctgccgctg atcagtcagt atctgccgct gcagtacgtg
     4981 aaagttcgct ccggcgagct gcagccaacg ccacgggaac tcattatcaa ccatattcag
     5041 gacatcctgg cgcagtacca cacagcctgt gaaggccaat aagcaaaaca aagaggaaca
     5101 cgctatgcca aatattgttt taagtcggat tgatgaacgc ttgattcacg gtcaggtcgg
     5161 cgttcagtgg gtcggatttg cgggggcaaa tctggtgctg gtagccaacg atgaggttgc
     5221 cgaagatccg gtacaacaaa acctgatgga aatggtactg gcagaaggga tcgccgtacg
     5281 tttctggacg ctgcaaaaag tcatcgacaa cattcatcgc gccgccgatc gacagaaaat
     5341 cctgctagtt tgtaaaacac cggccgattt cctgacgttg gtgaaaggtg gcgttccggt
     5401 gaatcgcatt aacgttggca atatgcacta cgccaatggc aagcaacaaa tcgccaaaac
     5461 ggtttctgtg gatgcgggtg atatcgcagc atttaacgat ctgaaagccg ctggggtgga
     5521 atgcttcgtt cagggcgtcc cgacagagcc tgctgtagac ctctttaaat tactttgagg
     5581 gattcatcat ggaaatcagt ctgttgcagg catttgcgtt gggaattatc gcctttatcg
     5641 ctggcctgga tatgtttaac ggcttaaccc atatgcaccg cccggtggtt ctcggcccgt
     5701 tggtcgggct ggtacttggc gatctgcata ccggtatttt aaccggcggt acgctggaac
     5761 tggtgtggat ggggctggcc cctctggcgg gcgcacagcc gcctaacgtg attatcggta
     5821 ctatcgtcgg cacggcgttt gccattacta ctggcgtgaa acccgatgtc gcggtcggtg
     5881 tcgccgtacc tttcgctgtc gcagtacaga tggggattac cttcctgttc tcggtgatgt
     5941 ccggcgtgat gtcccgatgc gaccggatgg cggagaacgc cgatactcgc ggcattgaac
     6001 gcgtgaacta tctggcattg ctggcactcg gcacgttcta ttttctctgc gctttcctgc
     6061 ctatctactt cggcgcggaa catgcaaaaa ccatcattga tgtcctgccg caacgattaa
     6121 ttgatggcct cggcgtcgcg ggcggcatca tgccagcaat cggttttgcc gtgctgctga
     6181 aaatcatgat gaaaaacgtc tacatcccct acttcatcct gggctttgtt gccgcggcct
     6241 ggctcaagtt accggtgctg gctattgctg ccgctgcgct ggcgatggcg ctgatcgacc
     6301 tgctgcgtaa atctcctgaa ccgactcaac ctgcggcaca gaaagaggaa tttgaagatg
     6361 gcatctaatc aaaccaccct gccgaacgtc agcgaaaatg aagaaacact gctgactggc
     6421 gtcaatgaaa acgtgtatga agatcagagc attggcgcgg agctgacgaa aaaagatatc
     6481 aaccgcgtcg cctggcgttc catgctgtta caggcttctt tcaactacga acgtatgcag
     6541 gcttccggct ggctgtacgg cctgctgcca gcactgaaaa agatccacac taataaacgc
     6601 gacctggcgc gcgccatgaa ggggcatatg ggtttcttca atacccatcc gtttctggtg
     6661 acgtttgtta tcggcattat ccttgcgatg gagcgttcta agcaggacgt taacagtatt
     6721 cagagcacca aaattgccgt cggtgcgccg ctcggcggga ttggcgatgc gatgttctgg
     6781 ctaacgctac tgccgatttg tggcgggata ggtgcaagcc tcgcgctaca aggctctatt
     6841 cttggcgctg tcgtctttat tgtgctgttc aacgtggtgc acctggggct gcgttttggt
     6901 ctggcgcatt atgcttaccg catgggcgtg gcggcgattc cactcattaa agcaaatacc
     6961 aaaaaagtcg gccatgcggc atctatcgtt gggatgacgg taatcggcgc gctggtggca
     7021 acctatgttc gtttaagcac cacgctggaa atcaccgcgg gcgacgcagt ggttaagtta
     7081 caggctgatg ttatcgacaa actgatgcca gccttcttac cgctggtcta caccctgacc
     7141 atgttctggc tggtacgccg cggctggagt ccgctgcgcc tgattgcagt gaccgtggtt
     7201 ctcggcatcg tcggtaaatt ctgccatttc ctttaaatac aatgaggtat tagatgttaa
     7261 gtattatttt gacagggcat ggcgggtttg ccagtggcat ggaaaaagca atgaagcaaa
     7321 tcctcggcga gcaatcgcag tttattgcca tcgattttcc ggaaacctca agcaccgcgc
     7381 tgcttacatc gcagcttgaa gaggccatcg cccaactgga ttgtgaagac ggcattgttt
     7441 ttctgacgga tttgctgggc ggcacgccgt ttcgcgtagc ttcaacgctg gcgatgcaaa
     7501 aactgggctg tgaagtgatt accggcacca atttgcaact gctactggag atggtgctgg
     7561 agcgcgaagg gttaagcggt gaagagtttc gtgtgcaggc gctggagtgt ggacatcgtg
     7621 ggctgaccag tctggtggac gagttaggcc gctgccatga ggaatgtccg gtcgaggaag
     7681 gaatatgaca cccgttttgc gcgccagaag gctgctgact gaagagggat ggctcgatga
     7741 ccatcagttg cgtattgctg acggtgtaat cgcagaaatc gaaccgattc cagtgggcgt
     7801 gactgaacgc gatgcggaac tgctctgccc cgcttacatc gacacccatg tacacggtgg
     7861 tgcgggcgtt gatgtaatgg atgacgcgcc ggatgtgctc gacaagctgg caatgcacaa
     7921 agcacgcgaa ggtgtcggca gttggttacc gaccaccgta accgcgccgc ttaataccat
     7981 tcatgcggcg ctggaacgta ttgctcaacg ttgccaacgc ggcggacctg gtgcacaaat
     8041 actggggagt tatctcgaag gaccgtactt cacgccgcag aataaaggcg cgcatccgcc
     8101 ggagctgttt cgcgagctgg aaattgccga gctggatcaa ttgattgccg tttctcagca
     8161 caccttgcgc gtggtggcgc tggcaccgga aaaagaaggg gcattgcagg ccatccgcca
     8221 tcttaaacag caaaacgtaa gggtgatgct ggggcatagc gcggcgacct ggcaacaaac
     8281 ccgcgccgcg tttgatgctg gtgccgacgg cctggtgcat tgctataacg ggatgacagg
     8341 tttacatcac cgcgaaccgg gaatggttgg cgcgggatta acggacaagc gcgcctggct
     8401 ggaactgata gccgatggtc atcatgtgca tccggcggcg atgtcgctgt gttgttgctg
     8461 tgcgaaagag agaatcgtgc tcatcaccga cgcgatgcag gcagccggga tgccggatgg
     8521 tcgctatacg ttatgtggcg aagaagtgca gatgcacggt ggcgttgtcc gtaccgcgtc
     8581 cggtggactg gcgggcagta cgctgtctgt cgatgcggca gtgcgcaaca tggtcgagtt
     8641 gacgggcgta acgcctgcgg aagccatcca tatggcgtcg ctgcatccgg cgcgtatgct
     8701 gggtgttgat gatgttctgg gatcgcttaa accgggcaaa cgcgccagcg tcgttgcgct
     8761 ggatagcggg ctgcatgcgc aacaaatctg gattcagggt caattagctt cgttttgatg
     8821 gtttgctcct ttattgggcc ttcacttccc ccgtaaggcc tttctttttc tttcgttttg
     8881 atctgtgcag cggtgtcgga tgcgacgcta atgcgcctta tccgacctac ggttggtgac
     8941 cgtaaggccg gataaagtat ttacgccgca tccagcaatc ccttttactt cccttgtctt
     9001 ttctttcaac gatcacagat ttcgttttat ttcctttttc tccattgaac tttcagtttc
     9061 ttttctatag attttaacca acgaaagaaa tcaccaagtg aaatgaaacg aaaggcaaac
     9121 gaaagcgaca acgccgacgt caagttcatc agactaagga ttgagttatg ccagaaaatt
     9181 acacccctgc tgctgccgca accggtacat ggactgaaga agagatccgc catcagcctc
     9241 gcgcatggat ccgttcactc accaacatcg acgcgctacg ttccgcgctc aataacttcc
     9301 ttgaaccgtt actgcgcaaa gagaatctgc aaatcatcct gaccggagcc ggaacatcgg
     9361 catttatcgg tgacatcatc gcgccgtggc tcgccagtca taccggtaaa aacttcagcg
     9421 ccgtaccgac caccgatctg gtcactaatc cgatggacta cctgaatcca gctcatccgc
     9481 tgctgttgat ctccttcggt cgatccggca acagcccgga aagcgtcgcc gccgtggaac
     9541 tggcaaatca atttgtaccc gagtgctatc acctgccgat cacctgcaac gaagcgggcg
     9601 ctctttacca aaacgcgatc aacagcgaca acgcgtttgc cctactgatg cccgcagaaa
     9661 cgcacgaccg cggctttgcg atgaccagca gcattaccac catgatggcc agttgcctcg
     9721 cggttttcgc acctgagacg atcaacagcc agagcttccg cgacgtggcg gatcgttgcc
     9781 aggcgatcct gacctcactg ggcgatttca gcgaaggtgt gtttggttac gcaccgtgga
     9841 aacggatcgt ttatctcggc agcggtggct tacagggcgc agcacgcgag tcggcgctga
     9901 aagtgctgga actgacagcg ggtaaactgg cggccttcta tgattccccg accggattcc
     9961 gtcatggccc gaaatcgctg gtcgataacg aaacgctggt ggtggtattt gtctccagcc
    10021 acccttacac ccgtcagtat gatcttgatc tgctggcaga actccgccgc gataaccagg
    10081 caatgcgcgt aatcgccatc gccgcggaaa gcaatgacgt tattactgcc ggtccacata
    10141 tcatcctgcc gccgtcccgt cactttatcg acgttgagca ggcattttgc ttcctgatgt
    10201 acgcccagac atttgcactg atgcagtcgc tgcacatggg taatacaccg gataccccat
    10261 cagccagtgg caccgttaac cgcgtggtgc aaggcgtaat cattcatccg tggcaggcat
    10321 aagaggatcg cattatgagc attatctcca ccaaatatct gttacaggac gcccaggcca
    10381 atggctacgc ggtgcctgcc tttaacattc acaacgccga gacgatccag gcgatcctcg
    10441 aagtgtgcag tgaaatgcga tcaccggtga tcctcgccgg gacgccgggg acctttaagc
    10501 atatcgcgct ggaagagatc tacgccctgt gtagcgccta ttccacaacc tacaacatgc
    10561 cgctggcact gcatctcgac caccacgaat cgctggatga tattcgccgt aaagtccacg
    10621 caggcgtgcg cagcgcgatg atcgacggca gtcacttccc gtttgccgag aacgtgaagc
    10681 tggtgaaatc ggttgtcgac ttctgccact cccaggattg cagcgtggaa gcagaactgg
    10741 gccgcctcgg cggtgttgaa gatgacatga gcgttgacgc cgaaagtgca ttcctgaccg
    10801 atccacaaga agctaaacgc tttgtcgaac tgactggcgt cgacagcctg gcggtagcga
    10861 ttggtacggc gcacggctta tacagcaaaa cgccgaagat tgatttccag cggctggcgg
    10921 aaattcgtga agtggtggat gttcctctgg tgctgcatgg tgccagcgat gtcccggatg
    10981 aatttgtccg tcgcactatt gaacttggcg tcacaaaagt gaacgttgcc acagaattaa
    11041 aaatagcctt cgctggcgcg gttaaagcct ggtttgcgga aaatccacag ggtaatgatc
    11101 ctcgttatta tatgcgcgtc ggtatggatg cgatgaaaga agttgtcaga aataaaatta
    11161 atgtctgtgg ttcagcgaat cgaatttcag cataatcatt agttttttat gatttatccc
    11221 aatgtacttc ccgattaa
//
LOCUS       DAZUCC010000165        11228 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZUCC010000165 DAZUCC010000000
VERSION     DAZUCC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.166"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            180..1838
                     /gene="ybjD"
                     /locus_tag="ACHY09_003745"
     CDS             180..1838
                     /gene="ybjD"
                     /locus_tag="ACHY09_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFS3886657.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1835..2791)
                     /gene="ybjX"
                     /locus_tag="ACHY09_003746"
     CDS             complement(1835..2791)
                     /gene="ybjX"
                     /locus_tag="ACHY09_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFS3886658.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYKGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2942..4057
                     /gene="macA"
                     /locus_tag="ACHY09_003747"
     CDS             2942..4057
                     /gene="macA"
                     /locus_tag="ACHY09_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFS3886659.1"
                     /translation="MKKRKTVKKRYVIALVIIIVGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4054..6000
                     /gene="macB"
                     /locus_tag="ACHY09_003748"
     CDS             4054..6000
                     /gene="macB"
                     /locus_tag="ACHY09_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFS3886660.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6073..6297)
                     /gene="cspD"
                     /locus_tag="ACHY09_003749"
     CDS             complement(6073..6297)
                     /gene="cspD"
                     /locus_tag="ACHY09_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFS3886661.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6447..6521
                     /gene="yljB"
                     /locus_tag="ACHY09_003750"
     CDS             6447..6521
                     /gene="yljB"
                     /locus_tag="ACHY09_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFS3886662.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6620..6940
                     /gene="clpS"
                     /locus_tag="ACHY09_003751"
     CDS             6620..6940
                     /gene="clpS"
                     /locus_tag="ACHY09_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFS3886663.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6971..9247
                     /gene="clpA"
                     /locus_tag="ACHY09_003752"
     CDS             6971..9247
                     /gene="clpA"
                     /locus_tag="ACHY09_003752"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFS3886664.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            9944..11152
                     /locus_tag="ACHY09_003753"
     CDS             9944..11152
                     /locus_tag="ACHY09_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3886665.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYVFPQLGAKTLDTITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQSRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHVLENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLDQRIPMMQAWADY
                     VMSQIMENQR"
BASE COUNT         2768 a         2580 c         3125 g         2755 t
ORIGIN      
        1 atgctaatta tttgattttg ttgtttttgc agacttatca gcaagaggga gtataacgcg
       61 attattcgct catttttcag acatttgcca tgcttaaatg tgatgtcatc acgtattagc
      121 atggcctttc ccgttatact gccagcgtaa aggataagtc acatatttct ggaggggata
      181 tgattcttga gcgcgttgaa attgtgggtt ttcgcggtat caaccgtttg tcgttgatgc
      241 tggaacaaaa caacgtcctg attggggaga acgcgtgggg taaatccagc ttgctggacg
      301 ccttaaccct gctgctatcg ccagaatcag atctctacca ttttgagcgc gacgatttct
      361 ggttcccgcc gggagatatc aacgggcgag aacatcatct gcatattatt ttgaccttcc
      421 gcgaatcgct gccaggccga catcgggttc gccgctatcg gccgctggaa gcgtgctgga
      481 cgccatgcac cgatggttat caccgtattt tttatcgtct ggaaggggag agtgcggaag
      541 acggcagcgt gatgacactg cgcagttttc tcgataaaga cggacatccg attgatgttg
      601 aggatattaa cgatcaggct cgtcatctgg tgcgtttaat gccggtgctg cgcttgcgtg
      661 atgcccgttt tatgcgccgt attcgtaacg gcacggtgcc aaatgtcccc aatgtggaag
      721 tcaccgcgcg ccagctcgat ttcctcgccc gtgaattatc ctcacatccg caaaatctct
      781 ctgatgggca gattcgtcag ggactttccg caatggtgca acttcttgag cattattttt
      841 ctgagcaggg agctggacag gcgcgatatc gtttaatgcg gcggcgagcc agcaatgagc
      901 aacgaagctg gcgctatctg gatatcatca accggatgat tgaccgacct ggagggcgct
      961 cgtatcgggt tattttgctc ggcctgtttg ctactttatt gcaggcaaaa ggcacattgc
     1021 gactggataa agacgcccgt ccattgttgc tgatcgaaga tccagaaacc cgtttacacc
     1081 ccattatgct ttcagttgcc tggcatctgt tgaatcttct gcccttgcag cgcattgcca
     1141 ccaccaactc gggtgagtta ctttcgttaa cgccggtaga gcatgtttgc cgactggtac
     1201 gtgagtcctc gcgcgttgcc gcctggcgtc tggggccgag tggcttgagt accgaagata
     1261 gccgacgcat atcctttcac attcgtttta accgtccgtc atcgctgttt gctcgctgct
     1321 ggctgctggt ggaaggggaa acggaaacct gggttatcaa tgaactggcg cgtcagtgcg
     1381 gacatcattt tgatgccgaa ggaatcaagg tcattgagtt tgcccagtcc gggctaaagc
     1441 cactggttaa atttgcccgc cgaatgggga ttgaatggca tgtactggtc gatggcgatg
     1501 aagcagggaa gaaatatgcc gctacggtac gcagcctgtt gaataacgat cgggaagccg
     1561 aacgagaaca tttaacggcg ttaccggcgc tggatatgga acattttatg tatcgccagg
     1621 gattttccga tgtgttccac cgcgtggcgc aaatcccgga aaatgtaccg atgaatctgc
     1681 gcaaaattat ctcgaaagcg atccatcgct cttccaaacc cgatcttgcc attgaagtgg
     1741 caatggaggc cggacgtcgt ggtgtggact ccgtaccgac gctgctgaaa aaaatgttct
     1801 cacgcgtgct gtggctggcg cgcggtcgcg cggattaacc gcgaaacatc gtggccattt
     1861 gtggctgaat agcgtcgagc atctcatagc gccgacggta ttcagcccgt tttttactgg
     1921 cgatttcggc aatctctttt cgtgctatct gtgccggaag gcggtaatgg cgttcagcat
     1981 cacatacgcc gccaaccgac tcccagaacg cgttgtaatc ggcatggatc ttgccttctt
     2041 tatcacgata acgcaggctg cggtaaatat gcgtttcatt gctgacggca ataatctgct
     2101 ctacctgcaa acgttgggca aacagacagg ccgcttccat cacaaggcgt ttgggaaata
     2161 gcccgtggca ggctttcgtc gcattctgga tttcctgatg tggaatttcc cattttgcgc
     2221 cttgcagtcc gccaataaac attgttcttt tccccttata ttcacacagg gtaaacgtga
     2281 tctctgccag aggaatacct tcgctgttgc ggaacaggat tgtgctgtca ccttctttat
     2341 ccattgagat catcatggtc agctcaagcg tgaactgctc gccgtttttg ccttccagtt
     2401 tagccagttg cagcccgggg gtattcaaat ataagctgaa ttcttccgcc gacatacacc
     2461 cgcggagtaa cgcataatgg taacgtaacg cctccagcaa ttgcttacgg ctaagattcg
     2521 ccgcaaggta agggcgatgc agacgcacag gcagtcgcgg ctggcgcgtt aacaacacat
     2581 tgagattggg ccagtgggaa agttcgttca tccactcaac gcttaaacgc ggcataatca
     2641 acgagcgcag caaaaatttc tggcgaaaac tacggcgatg ccagaattta cccggccgac
     2701 actgtccacg tgccagacta agaaaaagtg acaggctgct gagagattca gatggcgtaa
     2761 aggtccgttc agttagctgc gacatattca tgaaatcaat ggttatacat gacgtcgatt
     2821 tcaccattgc gtatcttaac caaacatcaa tagtgtgatt actaatgtaa attttagggt
     2881 tttgttgata tttcgttgaa gttaatgacc cggattggca tatggagtat tcagaatatt
     2941 tatgaaaaag cggaaaaccg tgaagaagcg ttacgttatt gcgctggtga taatcatcgt
     3001 cgggctgatt acgttatgga gaattcttaa cgcacccgtg ccgacttatc agacgctgat
     3061 tgtgcgcccc ggtgatttac agcaaagcgt gctggcgacc ggaaagctgg acgcgctgcg
     3121 taaggttgat gtgggcgcgc aggtcagcgg tcagttgaaa acgctgtcgg tggcgattgg
     3181 cgataaagta aaaaaagacc agcttttagg ggttattgat cctgaacagg ctgaaaacca
     3241 gattaaggag gtggaagcaa cgctgatgga gctacgcgcg cagcggcagc aggcggaagc
     3301 ggagctgaaa ctggcgcggg tgacgtattc ccgtcagcaa cgtcttgcac aaacgcaagc
     3361 ggtttcacag caggatctcg ataacgccgc gacggagatg gctgtgaaac aggcgcaaat
     3421 tggcaccatt gacgcgcaaa tcaagcgcaa tcaggcctct ctcgatacgg ctaaaaccaa
     3481 tctcgattac acccgcatcg ttgccccgat ggccggggaa gtcacgcaaa tcaccactct
     3541 gcaaggccag acggtgattg ccgcacaaca agcaccgaac attctgacgc tggcagatat
     3601 gagcaccatg ctggtaaaag cgcaggtttc tgaagcggat gtaatccacc tgaagccggg
     3661 gcaaaaagcc tggtttacgg tgcttggcga tccactgacg cgctacgagg ggcaaatcaa
     3721 ggatgtgcta ccgacgccgg aaaagattaa cgacgctatt ttctattacg cccgttttga
     3781 agtacccaac cccaatggtt tactgcggct ggatatgacc gcgcaagtgc atattcagct
     3841 caccgatgtg aaaaatgtgc tgacgatccc tctgtcggcg ttaggcgatc cggttggcga
     3901 taatcgttat aaagtcaaat tgttgcgtaa tggtgaaaca cgcgagcgtg aagtgacgat
     3961 tggcgcacgt aacgataccg atgttgagat tgtcaaaggg ctggaagcgg gcgatgaagt
     4021 ggtgattggt gaggccaaac caggagctgc gcaatgacac ccttgctcga attaaaggat
     4081 attcgtcgca gctatcctgc cggtgatgag caggttgagg tgctgaaggg catcagcctc
     4141 gatatttatg ctggtgagat ggtggcgatt gtcggcgctt cgggttccgg taaatcgacc
     4201 ctgatgaata ttctcggctg cctggataag gccaccagcg gcacctatcg cgtagccggt
     4261 caggatgttg ccacgctgga cgccgatgcg ctggcgcaac tacgccgcga gcatttcggc
     4321 tttattttcc agcgttacca tttgctttcg catttaaccg ccgagcagaa cgttgaagta
     4381 cccgccgtct atgccggtct tgagcggaaa cagcggctgc ttcgcgccca ggagttgctg
     4441 caacgactgg ggctggaaga ccgtacagag tattacccgg cacagctttc gggtggtcag
     4501 caacagcgcg tcagcatagc gcgggcattg atgaacggcg gtcaggtaat tcttgccgat
     4561 gaaccgaccg gcgcactgga cagccattct ggcgaagagg tgatggcgat cctgcatcag
     4621 ctgcgcgatc gcgggcatac ggtgattatc gttacccacg atccgcaggt cgctgctcag
     4681 gccgagcggg tgatcgaaat tcgcgacggt gaaattgtgc gcaatcctcc cgccattgaa
     4741 aaagtgaatg ttgctggcgg gacggaacct gttgtcaaca cggcgtctgg ctggcggcag
     4801 tttgtcagcg gttttaacga ggcgctgacg atggcatggc gggcgctggc agcgaataaa
     4861 atgcgtactt tactgaccat gctggggatt attatcggta ttgcgtcggt ggtttccatt
     4921 gtcgtggtgg gtgacgccgc caaacaaatg gtgctggcgg atattcgttc tattggtacg
     4981 aatactattg atgtctatcc cgggaaagat tttggcgatg acgatccgca atatcagcag
     5041 gcgctgaagt acgacgactt aatcgccatc caaaaacaac cgtgggtcgc ctcagccaca
     5101 cctgccgtct cgcaaaacct gcgcctgcgt tataacaatg ttgatgtggc tgccagtgcc
     5161 aatggcgtga gcggcgatta ttttaatgtc tatggcatga ccttcagtga agggaacacc
     5221 tttaatcagg agcagctgaa cggtcgtgcg caggtggtgg tgctcgacag taatactcgc
     5281 cgccagcttt tcccccataa agcagatgtg gttggcgagg tgattctggt cggcaatatg
     5341 cccgccagag tcattggtgt ggcggaagaa aaacagtcga tgtttggtag cagtaaagtg
     5401 ctgcgtgtct ggctacctta cagcacgatg tccgggcgag ttatgggcca gtcgtggctg
     5461 aactccatta ctgtcagggt gaaagaaggc tttgacagcg ctgaggcgga acagcaactc
     5521 acgcgtttac tttcactgcg ccacggtaag aaggatttct ttacctggaa catggacggc
     5581 gtcttgaaaa ccgttgaaaa gaccacacgt actttacaac tgtttctgac gctggtggcg
     5641 gtgatttcgc tggtggtggg cggtattggt gtaatgaata ttatgctggt gtcagtgacc
     5701 gagcggacgc gggaaattgg cattcgcatg gctgtaggtg cgcgagcaag cgatgttttg
     5761 caacagttcc tgattgaagc cgtactggtt tgcctggtcg gtggcgcgtt gggaataaca
     5821 ctgtcactgt taattgcttt caccttacag cttttcttac ccggctggga gattggtttt
     5881 tcaccgttgg cgctgctgct ggcgtttctc tgctcgacgg tcaccgggat tttatttggc
     5941 tggttacccg cacgaaatgc ggcacgactg gatccagtag atgctctggc acgagagtaa
     6001 tttttgagat aaaaatgcca gccgatcggg ctggcatttt gcctttagga tgtacacaat
     6061 gagacagaag agctatgcga ctgccgcttc tacttcgacg ggcacaataa cactggcgtg
     6121 attgcctttt ggcccctggt ggacatcaaa ctgaacggat tgtccagctt ttagcgttct
     6181 gtaaccatcc atctgaatgg tggaataatg agcgaaaata tcttcgccgc cgccttcagg
     6241 gcagatgaaa ccaaaccctt tggcattgtt gaaccactta acagtaccct tttccatgct
     6301 tcgacatcct tcgcaaatct tatacaagta agatggaata aaccggggtc agagaggggg
     6361 ctgttcaaaa cctcgccaac tctagaaata caatttagag aattagggcg agccgtcaag
     6421 catttgacag gggccagggg gcaggtatga atcaaaaatt tgaagcagtt aacgctattg
     6481 acaggaatgt gacagatgtc gctgatgcca acgatagatg atagttatct atcatgtgga
     6541 gtagattggt caggcaaata agctcttgtc agcggcaggg cgttctgccg ataaccgtaa
     6601 ccgaagatga taactgacaa tgggtaaaac gaacgactgg ctggactttg atcaactggc
     6661 ggaagaaaaa gttcgcgacg cgctaaaacc gccatctatg tataaagtga tattagtcaa
     6721 tgatgattat actccgatgg agtttgttat tgacgtgtta caaaaattct tttcttatga
     6781 tgtagaacgt gcaacgcaat tgatgctcgc tgttcactac caggggaagg ccatttgtgg
     6841 agtctttacc gctgaggttg cagaaaccaa agtggcgatg gtgaacaagt acgcgaggga
     6901 gaatgagcat ccattgctgt gtacgctaga aaaagcctga atgcaggcat aaaaattggg
     6961 ggaggtgcct atgctcaatc aagaactgga actcagttta aatatggctt tcgccagagc
     7021 gcgcgagcac cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc tgctcagtaa
     7081 cccatctgcc cgggaggcgc tggaagcgtg ctctgtggat ttggttgcgc tccgtcagga
     7141 actggaagcc tttattgaac aaaccacacc cgttctgcct gccagtgaag aggagcgcga
     7201 cacacagccg acgctgagtt ttcagcgtgt actgcaacgt gcggtcttcc atgtccagtc
     7261 ctccggtcgc aatgaggtta ccggtgcaaa cgttctggtc gctatcttta gcgaacagga
     7321 gtcgcaggcg gcatatctgt tgcgcaaaca tgaagtcagc cgtctcgatg tggtgaactt
     7381 tatctctcat ggcacgcgta aagacgagcc gacacagtct tctgatcctg gcagccagcc
     7441 aaacagcgaa gaacaagctg gtggggagga acgtatggag aatttcacga cgaacctgaa
     7501 tcagcttgcg cgagtgggcg gaatcgaccc actgattggt cgtgagaagg agctggagcg
     7561 tgctattcag gttctctgcc gtcgccgtaa aaacaacccg ctgctggtgg gggaatctgg
     7621 tgtcggtaaa accgcgattg cggaggggct tgcatggcga attgttcagg gcgatgtgcc
     7681 ggaagtgatg gctgactgta cgatttactc tctcgatatc ggttctctgt tagcgggcac
     7741 aaaatatcgc ggcgactttg aaaaacgttt taaagcgttg ctcaagcagc tggagcaaga
     7801 cactaacagc atcctgttta ttgatgagat ccacaccatt atcggtgcgg gtgcagcgtc
     7861 tggtggccag gtcgatgcag ctaacctgat caaaccgttg ctctccagcg gtaaaattcg
     7921 cgtaattggt tcgacaacct atcaggagtt cagcaacatt ttcgagaaag accgtgctct
     7981 ggcgcgtcgc ttccagaaaa ttgatattac tgaaccgtca atcgaagaaa ctgttcaaat
     8041 catcaatggc ctgaaaccga agtatgaagc gcaccacgac gtgcgttata ccgcaaaagc
     8101 ggtgcgtgca gcggtagagc tggcggtgaa atacattaac gatcgtcatc tgccggataa
     8161 agccattgac gttatcgacg aagcgggcgc tcgcgcacgc ctgatgccgg taagcaaacg
     8221 caagaaaacc gttaatgtgg cggatattga gtccgtggtg gcccgtattg cgcgcattcc
     8281 agagaagagt gtttcgcaga gtgaccgcga taccctgaaa aacctcggcg atcgcctgaa
     8341 aatgctggtc ttcggtcagg ataaagccat tgaggcgctg actgaagcca ttaagatggc
     8401 gcgtgcaggt ttaggtcacg aacataaacc ggtcggttcg ttcctgtttg ccggtcctac
     8461 cggggtcggg aaaacagagg tgacggtaca gctttcgaaa gcgttgggca ttgagcttct
     8521 gcgctttgat atgtccgagt atatggaacg ccataccgtc agccgtctga ttggtgcgcc
     8581 tccgggatac gttggttttg atcagggcgg tttgctgact gatgcggtca tcaagcatcc
     8641 acatgcggtg ctgctgctgg acgaaatcga gaaagcgcac ccggacgtgt tcaatattct
     8701 gttgcaggtg atggacaacg gtacgctgac cgataacaac ggacgcaaag cggacttccg
     8761 taacgtggta ttggtgatga ccaccaacgc tggggtacga gaaactgagc gtaaatcgat
     8821 tggtcttatc caccaggaca acagtaccga tgcgatggaa gagatcaaga agatctttac
     8881 gccggagttt cgtaaccgtc tcgacaacat tatctggttc gatcatctct ccaccgacgt
     8941 gatccatcag gtagtggata aattcatcgt cgagttgcag gttcagctgg atcagaaagg
     9001 tgtttctctg gaagtgagcc aggaagcgcg taactggctg gccgagaaag gttacgaccg
     9061 ggcaatgggc gcacgtccga tggcgcgtgt catccaggac aacctgaaaa aaccgctcgc
     9121 caacgaactg ttgtttggtt cgctggtgga cggcggtcag gtgacggttg cgctggataa
     9181 agagaaaaat gagctgactt atggattcca gagtgcacaa aagcacaagg cggaagcagc
     9241 gcattaatct aattgttagg taggttggtg aagtccgtaa tctcgaaaga ggttacggac
     9301 tttttgttta tggggtggag gaggttctga cccttttttt gatgatgatg gtaatttgtt
     9361 gataattagt gctgcgggaa ggtaaggata aaaaagggtg gtagcaggaa aatgagatgg
     9421 ttttgcttta ttaacaacgg gctagacgtg tagtatttga gttcactgcc gtacaggcag
     9481 cttagaaagc caaccgaaag aaaaactcga tttttatacc gttcactgcc gtacagacag
     9541 ataaaatgcg aaaaaaaaaa cgtactttcg tacgagctct tctttgaata tggcggtgag
     9601 ggggggattg actcgcttcg ctcgccctgc gggcagccca ctcactgtgt tcgtggtctg
     9661 tccaactggc tgcgccagtt gtcgaacccc ggtcggggct tctcatcccc cctggagtgc
     9721 aatatgcgaa aaaaaagctc gcactttcgt acgagctctt ctttgaatat ggcggtgagg
     9781 gggaccgcct gaaataaatc tgacttaatc cgggagtgtt atataacttc attataaaca
     9841 atgcattaca atttttgctg tccgatgtta tctaatgtat tcttagtgaa gtgagagact
     9901 aatggcatac tggatggcat accagagtaa caggaattag cgtatggcag tattgacgga
     9961 tactaaagca aggcatatca aacctgatga caaaccattg ccccatgggg gaattacagg
    10021 actgactctt catccttctt cagtaaaggg gcgggggaaa tgggtttttc gttatgtaag
    10081 tccggtgaca caaaaaaggc gtaatgctgg attgggtact taccctgagg tcagtattgc
    10141 tgaagctgca cgtactgccc ggataatgcg agagcaactt gctgcaggtg atgatcctct
    10201 ggagattaaa aaggctgaag ctgaaaaagt tgttatccca acatttgccg atgcagcccg
    10261 gcgtgtacat gcagaattgt caccgggatg ggaaaaccca aagcatgtca ggcagtggtt
    10321 gtcgacgctt gaaaattacg tgtttcctca actgggagca aaaacgctgg atacgattac
    10381 ggctgcggac gtggcagaga cactgcgtcc agtctggttg accttatcag aaacggcaag
    10441 ccgggttaaa cagcgcattc atgttgttat gcagtggggc tgggcgcatg gtttttgtgt
    10501 ggcgaatcct gttgatgtgg ttgatcattt gcttccacag caatcaagag gacgtgatga
    10561 acaccagccg gcaatgccct ggaggcagtt accgcttttt gtggcgacca gtgtgtatac
    10621 agatgaacct tataatgtta cccgggcact gttattaatg gtgatactga cagcaacccg
    10681 ctcgggcgaa gcaaggggaa tgcgctgggc tgaaattgat tttcataagc ggatatggac
    10741 gatacccgca gaaagaatga aagccaggat acagcatcgt gttcctttat cccgacaggc
    10801 cattcacgtt ctggaaaata tacgtggtct gcatgacgaa ctggtgtttc cttctcccag
    10861 aaagcagcag atcctttcag atatggtgtt gacgagtttt ctacgtaaaa agaaggccat
    10921 cagtgatata cccggacgag tggctacagc acatggtttt cgttcaacat tcagggactg
    10981 gtgtagcgaa cagggatatt cgcgggattt ggcggaaagg gcgcttgccc acacgctgaa
    11041 aaataaggtt gaggcggcat atcaccggac tgatctgctg gatcagcgta taccgatgat
    11101 gcaggcatgg gcggattatg tgatgtctca gattatggaa aaccagcgat gaaatcacat
    11161 ctcacagatt tgcagatgtc gtctgtaatc acgaaaaata tacttattga gttataatgc
    11221 ttgtttgt
//
LOCUS       DAZUCC010000166        11189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZUCC010000166 DAZUCC010000000
VERSION     DAZUCC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.167"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..1288
                     /gene="manD"
                     /locus_tag="ACHY09_003754"
     CDS             74..1288
                     /gene="manD"
                     /locus_tag="ACHY09_003754"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFS3886666.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTASGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1355..2638
                     /locus_tag="ACHY09_003755"
     CDS             1355..2638
                     /locus_tag="ACHY09_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3886667.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2890..3807
                     /gene="oxyR"
                     /locus_tag="ACHY09_003756"
     CDS             2890..3807
                     /gene="oxyR"
                     /locus_tag="ACHY09_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFS3886668.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3790..5190)
                     /gene="sthA"
                     /locus_tag="ACHY09_003757"
     CDS             complement(3790..5190)
                     /gene="sthA"
                     /locus_tag="ACHY09_003757"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFS3886669.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5522..6172
                     /gene="fabR"
                     /locus_tag="ACHY09_003758"
     CDS             5522..6172
                     /gene="fabR"
                     /locus_tag="ACHY09_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFS3886670.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6172..6531
                     /gene="yijD"
                     /locus_tag="ACHY09_003759"
     CDS             6172..6531
                     /gene="yijD"
                     /locus_tag="ACHY09_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFS3886671.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLL
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6571..7671)
                     /gene="trmA"
                     /locus_tag="ACHY09_003760"
     CDS             complement(6571..7671)
                     /gene="trmA"
                     /locus_tag="ACHY09_003760"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFS3886672.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7785..7975
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8040..9923
                     /gene="btuB"
                     /locus_tag="ACHY09_003761"
     CDS             8040..9923
                     /gene="btuB"
                     /locus_tag="ACHY09_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFS3886673.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     gene            9868..10725
                     /gene="murI"
                     /locus_tag="ACHY09_003762"
     CDS             9868..10725
                     /gene="murI"
                     /locus_tag="ACHY09_003762"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS3886674.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            11098..>11189
                     /locus_tag="ACHY09_003763"
     rRNA            11098..>11189
                     /locus_tag="ACHY09_003763"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2650 a         2615 c         2959 g         2965 t
ORIGIN      
        1 ctttctctct tgtatggtag taggcgcaaa attaagtttg aaacgtctat ttacaacaca
       61 ggagattgca ataatgaaaa ttattgctgc agatgttttt gttacttgcc cagggcgtaa
      121 tttcgttacg cttaaaataa ccactgcaag cggactttgt ggattaggag atgccacgtt
      181 aaatggtcgc gaattgtcgg tcgcttctta tctgaaagat catctttgtc cacaactaat
      241 tggccgcgat gccagccgta tcgaagatat ctggcaattc ttttataaag gtgcttactg
      301 gcgacgtggc cccgtcacga tgtcagccat ttctgccatt gatatggcgt tgtgggatat
      361 caaagcaaaa gcggcaaata tgccattgta tcaactgctt ggtggtgctt ctcgtgaagg
      421 cgttatggtt tattgccaca ccaccgggcg tacgattgat gaagtccttg aagactatgc
      481 aaagcaccag cagatgggct ttaaggcgat tcgtgtgcag tgtggtgtac cgggtatgca
      541 aaccacctat ggtctggcga agggcaaagg actggcgtat gaaccagcaa ccaaaggcct
      601 gtggccagaa gagcagttgt ggtcttcaga aaagtatctc gattttacgc cgaagttatt
      661 tgaagcggtg cgtaataagt tcggttttaa cgaacatctg ttgcatgaca tgcaccatcg
      721 cctgaccccc atcgaagcgg cgcgctttgg gaagagcatt gaagattacc gcctgttctg
      781 gatggaagat ccgacgcctg ccgagaatca ggaatgtttt cgtctgattc gtcagcatac
      841 cgtgacgccg attgcggtcg gtgaagtgtt taacagtatc tgggactgca agcagttaat
      901 cgaagagcag ctgattgact atattcgcac gacgattacc catgcaggcg gcattaccgg
      961 gatgcgacgg attgccgatt ttgcatcgct ttatcaggta cgtaccggtt cgcatggccc
     1021 ttcagattta tcgcctgttt gccatgcggc ggcgctgcat tttgacctct gggtgccaaa
     1081 ctttggcgtt caggagtaca tgggctattc cgaacaaatg cttgaagtct tcccgcatag
     1141 ctggcgcttc gatgaaggtt atatgcaccc aggcgatgaa ccggggctgg gtatttcttt
     1201 cgatgagaaa ctggcggcga aatatccgta tgacccggcg tatctccccg tcgcacgact
     1261 ggaagatggc acattgtgga actggtaata gcggttcgct taattatttt ctgaatattc
     1321 atcaaatcga ttaattccgc tcaaggagct aaaaatggac gttgatgtgt caaccagtgt
     1381 tgcgggtaat aaaccgcaac ggattcgtcg tattcagaca gtgaccctgg ttttattatt
     1441 tatggcgggg atcgttaatt ttctcgaccg ttcgtcattg agcgtggcag gtgaagcaat
     1501 tcgtggcgag ttaggattat ccgctaccga gtttggtgtt ttgctttctg cattttctct
     1561 gtcttatggt ttttcacaac ttccttccgg tattttgcta gaccgctttg gcccacgaat
     1621 agtgttaggc gcaggcttaa tattctggtc attaatgcag gcattaacag gaatggttaa
     1681 ttcttttagc cactttattt taatgcgtat cggtctgggt attggcgaag cgccatttat
     1741 gcctgcggga gtaaagtcga tcaccgactg gtatgcgcaa aaagaacgcg gcacggcgct
     1801 ggggattttt aactcatcta ccgttatcgg tcaggctatc gcgcctcctg ctcttgtatt
     1861 gatgcaactg gcatggggct ggcggacgat gtttgtcatc atcggcgtag cagggattct
     1921 ggttgggatc tgttggtacg cgtggtatcg caaccgggcg cagtttgtcc tgaccgagga
     1981 agaacggaca tatctctccg cgccggttaa accgcgtcca cagttacaat ttagcgagtg
     2041 gctggcgctg tttaagcatc ggacaacctg ggggatgatt ttaggtttct ctggcgtcaa
     2101 ttataccggt tggctctaca tcgcgtggtt acccggttat ttgcaggccg aacaaggttt
     2161 cagtctggcg aaaaccggtt gggtggcggc aattcctttc ctcgcggcgg cggtcgggat
     2221 gtgggttaac ggtattgttg tcgatcggct ggcgaaaaaa ggctatgacc tggcgaagac
     2281 gcgtaaaacg gcgattgtct gcggtttgat gatgtcggca ttaggcacgt tgctggtcgt
     2341 gcaatcttcc tcgccagccc aggcggtagc gtttatctca atggcgctgt tctgcgtgca
     2401 tttcgcggga acatctgcat gggggctggt gcaggtgatg gtgtcagaaa caaaagtggc
     2461 ttccatcgcc gggattcaaa actttggcag ttttgttttt gcttcctttg ctccgatcgt
     2521 aaccggttgg gtcgtggata ccacacactc gtttaatctg gcgctggtta tcgcggcctg
     2581 tgtgacgttc accggggcgc tgtgttactt ctttattgtc aaagatcgga ttgagtaacc
     2641 gttactatca ggctctcttg ctgtgggcct gtagaataaa aaaaagcgga tcctggagat
     2701 ccgcaaaagt tcacgttggc tttagttatt cgagttgaga aactctcgaa acgggcagtg
     2761 acttcaaggg ttaaaagagg tgccgctccg tttctgtgag caattatcag tcagaatgct
     2821 tgatagggat aatcgttcgt tgctatgcta cctatcgccg cgaactatcg tggcaatgga
     2881 ggatggataa tgaatattcg tgatcttgag tacctggtgg cattggccga acaccgccat
     2941 tttcggcgtg cggcagattc ctgccacgtt agccagccga cgcttagcgg gcaaattcgt
     3001 aagctggaag atgagttggg tgtgatgttg ctggagcgga ccagccgtaa agtgttattc
     3061 acccaggcgg gaatgctgct ggtggatcag gcgcgtaccg tgctgcgtga ggtgaaagtc
     3121 cttaaagaga tggcaagcca gcagggcgag acgatgtccg gaccgctgca tattggtctg
     3181 attcccacgg ttggaccgta cctgctaccg catattatcc cgatgctgca ccagaccttt
     3241 ccaaagctgg aaatgtatct gcatgaagca cagacccacc agttactggc gcaactggac
     3301 agcggcaaac tcgattgcgt gatcctcgcg ctggtgaaag agagcgaagc attcattgaa
     3361 gtgccgttgt ttgatgagcc aatgttgctg gctatctatg aagaccatcc gtgggcgaac
     3421 cgcgaatgcg taccgatggc cgatctggca ggggaaaaac tgctgatgct ggaagatggt
     3481 cactgtttgc gcgatcaggc aatgggtttc tgttttgaag ccggggcgga tgaagataca
     3541 cacttccgcg cgaccagcct ggaaactctg cgcaacatgg tggcggcagg tagcgggatc
     3601 actttactgc ctgcgctggc tgtgccgccg gaacgcaaac gtgatggggt tgtttatctg
     3661 ccatgcatta agccggaacc acgccgcact attggcctgg tttatcgtcc tggctcacca
     3721 ctgcgcagcc gctatgagca gctggcagag gccatccgcg caagaatgga tggccatttc
     3781 gataaagtgt taaaacaggc ggtttaagcc atttagcgca gctacccgat aggcttccgc
     3841 catcgtcggg tagttaaagg tggtgttgac gaagtactca atagtgttgc cgccaccttt
     3901 ctgttccata atcgcctgac cgatatgaat aatttcggca gcgcgctcgc caaagcagtg
     3961 aatacccaga atctcttttg tttcccgatg gaacaaaatt ttcagcgtgc ccacgttcat
     4021 gccgacgatt tgtgcacgtg ccagatgttt aaactgggcg cggcccactt catatggcac
     4081 tttcatcgcg gtcagctgct gttcggtttt gcccacagag ctgatttccg ggatggtgta
     4141 aataccggta gggatatctt caatcagatg tgcggtggct tcacctttca ccagcgcctg
     4201 cgcggcaatg cgcccctggt cataggccgc cgacgccagg ctcggataac caatcacgtc
     4261 gcccaccgcg taaacgtgcg gctgtgcggt ctgatacatg ctgttgacct tcagctgtcc
     4321 gcggctgtca gtttccagcc caatgttctg taacgccagc gaatcggtat taccggtgcg
     4381 accgttggca tagagcaggc agtcagcttt cagtttttta cccgacttca aatgcatgat
     4441 cacaccgtcg tcacagcctt cgatcttctc gtactcttcg ttgtgacgaa tcactacgcc
     4501 actgttccag aagtgatagg agagagaatc tgacatctct tgatcgagaa atgccagcag
     4561 gcgatcgcgg gtgttgatca gatccacttt tacatccata ccgcggaaga tcgacgcata
     4621 ttcacagccg atcactccag caccatagat aagtacatgg cgcggttcgt ggtgcatgct
     4681 aagaattgag tcgctgtcgt aaatgcgtgg atgcgtgaaa tcaacatctg ttggatgata
     4741 tggacgagag ccgcaagcaa taacaaattt ttcagcggtt agtgtctcga cgctgccgtc
     4801 cgggcaatcc agcgccagcg tgtgctcgtc aacaaagcga gcgttcccct gcaatatttc
     4861 acagtgatta cgttcgtaaa atccctgacg catgcgcgtt tgttgattaa tcacgttatc
     4921 ggcatggtta aggatatcgg caaaagaaga gcggagcagt cgcgaatggt cgctgtaaag
     4981 tgggttttga ttaaattcta taatgcggct gacggcgtga cggagagctt tcgacgggat
     5041 ggtgccccag tgggtgcaac cgccgccaac attttgataa cgctcgataa ctgcgacgcg
     5101 cgcaccttgc ttaaccaggc ccattgcagc gccttcgccg ccggggccgg aacctattac
     5161 tatggcatcg taatcgtagg aatgtggcat ggtagggctt acctgttctt atacataaaa
     5221 gcaacagaat ggtaacattt tatcgcgggt aagccaattg atccccgtca tttatctggc
     5281 tatatcgtga gcggcctttg ctttgtctgt ttcttacttt tgacctgaac gttttattgg
     5341 atttttatcg acgatactct ccgtttaagc ggcaggtttc cgttgtacgt aaaagaaccg
     5401 gccaaagaat tgcagtaaat atgttttatt gcgttaccgt tcattcacaa tactggagca
     5461 atccagtatg ttcattctct ggtatagtgc cagcagtact tttggcaagg attcagacat
     5521 cgtgatgggc gtaagagcgc aacaaaaaga aaaaacccgc cgttcgctgg tggaagccgc
     5581 atttagccaa ttaagtgctg aacgcagctt cgccagcctg agtttgcgtg aagtggcgcg
     5641 tgaagcgggc attgctccca cctcttttta tcggcatttc cgcgacgtag acgaactggg
     5701 tctgaccatg gttgatgaga gcggtttaat gctacgccaa ctcatgcgtc aggcgcgtca
     5761 gcgtatcgcc aaaggcggga gtgtgatccg cacctcggtc tccacattta tggagttcat
     5821 cggtaataat cctaacgcct tccggttatt attgcgggaa cgctccggca cctccgctgc
     5881 gtttcgtgcc gccgttgcgc gtgaaattca gcacttcatt gcggaacttg cggactatct
     5941 ggaactcgaa aaccatatgc cgcgtgcgtt tactgaagcg caagccgaag caatggtgac
     6001 gattgtcttc agtgcggggg ccgaggcgtt ggatgtcggc gtcgaacaac gtcggcaatt
     6061 agaagagcga ctggtactgc aactgcgaat gatttctaaa ggggcttatt actggtatcg
     6121 ccgtgaacaa gagaaaaccg caattattcc gggaaatgtg aaggacgagt aatgaaacaa
     6181 gcaaatcaag atagaggtac gctgctgctg gcgttggttg ctggcttatc gattaatggt
     6241 actttcgcag cgctgtttag ctccattgtg ccattttctg tattcccgat tatttccctg
     6301 ttgctgacgg tttactgcct gcatcaacgt tatcttaatc gcaccatgcc ggtaggcttg
     6361 ccgggtctgg cagctgcctg ttttattctc ggcgtactgc tgtacagcac ggtagttcgt
     6421 gcggaatatc cggatatcgg ctctaacttc ttcccggcag tactctccgt cattatggtg
     6481 ttctggattg gcgcgaagat gcgtaaccgt aagcaggaag ttgctgagta atcggtatca
     6541 tgccggatgc tggtgcatcc ggcatgggtt ttacttcgcg gtcagtaata cgccgcactc
     6601 catatggtgc gtgtagggga actgatcaaa cagagccaga cgttcgacct tgtgcgtctg
     6661 gcttaatgtt tccagattct tgcataacgt ttccggattg caggagatat acagaatacg
     6721 tggatacgcc tgcaccattt tctcggtttc actgtccaga ccgctgcgcg gtgggtcgac
     6781 aaaaatggtt tcgcactgat aactctttaa gtcgattcct tgcaggcggt taaactcgcg
     6841 tacgccattc atcgcctgag taaattcttc tgccgccata cgaataattt gtacgttatc
     6901 aatatggtta gcggcaatgt tgtattgcgc ggcagcgacc gacggcttag cgatttcggt
     6961 ggctaatacc cgatcaaaat tgcgcgccag tgctaatgaa aagttaccgt tgccgcagta
     7021 cagctccagc aaatcgcctt ttgagccttt ggttacgtcc agcgcccatt ccagcatctg
     7081 aatattcatc gccgcgttcg gctgggtaaa gctgttttct acctgacggt agatcatctc
     7141 tttccctgcg accggcagac gttcatcgat gtaatcctgg tccagctcga ttttggtttt
     7201 cgttgcccga ccaatcagat gcacattcag attctgcgct cgcagtgcat cgcgcagggc
     7261 ttccgcttcc tgacgccact catcatccag cttcttatgg tacagcaggg aaaccaccgc
     7321 ctgattactc agcgtggtga ggtaatcaat ctggaacaac ttgtggcgca gaacgggatt
     7381 attacgcaca cccgcaatca tcgccgtcat caactggttg ataagttcac tggcggcggg
     7441 gaagctatcc acgcggatgc ggcttttggt ttgttgatcg aaaatgatgt gatacaggtc
     7501 atcgccatcg tgccagatgc ggaactccgc acgcatccgg taatgactga ccggcgagcg
     7561 aaacacttcc ggaaccaggt cagaaaacgg tgccatcata ctttgcaaac gtaccacttt
     7621 ttcggctaac tgcgcttcat actgttctgt tggaaggtgt tcgggggtca tgatgtatcc
     7681 tgaaaaatta aagtacgcgg cgattgtagg gattgctcat cagatgtcca gatcttgatg
     7741 aattcctatt tgtgagctac gtctggacag taacttgtta caacctgtag catccacttg
     7801 ccggtcctgt gagttaatag ggaatccagt gcgaatctgg agctgacgcg cagcggtaag
     7861 gaaaggtgcg atgattgcgt tatgcggaca ctgccatccg gtgggaagtc atcatctctt
     7921 agtatcttag atacccctcc aagcccgaag acctgccggc taacgtcgca tctggttctc
     7981 atcatcgcgt aatattgatg aaacctgcgg catccttctt ctattgtgga tgctttacaa
     8041 tgattaaaaa agcttcgctg ctgacggcgt gttctgtcac agccttttcc gcttgggcac
     8101 aggataccag cccggatact ctcgttgtta ctgctaaccg ttttcagcag ccagtaaata
     8161 ctgtccttgc acctactgat attgttaccc gtgaggatat tcaacgctgg cagtcgaaag
     8221 acctcaacga tgttatgagt catctccccg gtgtaaatat ctctcaaact ggtggaatgg
     8281 ggacgagttc ttctctttat gttcgaggca ctgaatcgcg ccatgtttta gtgttgattg
     8341 acggtgtgcc gatggcccgt gccggtatcg acaatgcaat tgatattagc cagttcccgg
     8401 tatcacttgt acagcgtgtt gaatatattc gtgggccgcg ttctgccgtt tatggttctg
     8461 gtgccatcgg aggtgtggtg aacattatta caatgagtaa tgatgagaaa gctcagatca
     8521 acgcaggaat gggtacaaat ggttatcaag cctatgatgg ggtaataaat aaacgttttg
     8581 gcgatacgat ggtaacagca gcaggtgctt accagacgac caaaggtttt aacattcagc
     8641 ctggctcacc atacagtggt gatagcgatc gcgatggata tcgcaacaaa cttttctggg
     8701 gggggctgca gcataagttt aacgataact tctcggggtt cttccgtggt tacagctaca
     8761 cagctaataa tgattacgac cagggctcga tgtatggttc tgctactggc aacgaagaag
     8821 ctcagaatta caaccagtcc tgggatactg gcctacgcta taactccggc atctattcgt
     8881 ctcagttaat tgctaactat caacgactca agaattataa ctacacaaat aatcttggtc
     8941 gttatgcggg agatgccacc ctggatgaca tggaacagcg ttatattcag tggggtaata
     9001 gcattgaggt agggcatggt gcaattagcg gcgggatcga ttggaaacag gaaaaactga
     9061 cgtccagcag cattacaaaa tcagatgatt acgaacgcga taccaccggg ttatacctga
     9121 ctggacagca acagatttcc aatgtgactt tggaagcttc tggccgagaa gatcatgatg
     9181 aacagtttgg ctggcatggt acctggcaga cagctgctgg ctgggagttt attgatggct
     9241 accaggcaac actttcttac ggtaccagtt ttctttcgcc ttctcttgga cagcaatacg
     9301 gggctgctcg ctttgcttca atttatggtc ctggtattgc agccaaccca aatttgaagc
     9361 cagaagaatc taaacagtgg gagattggaa ttgagggggt aactgggact ctggattggc
     9421 ggctctcagg atatcgttat gaaattcaga acctgattga ttataaaagc ataaataatg
     9481 tcaaccagta cattaatgtg aaatcagcca cgattaaagg gctggaatgg acgggaaata
     9541 ttacaaccgg tcctgttgaa catcgcttga cgttacaata tgttgacccg cgcgatgatg
     9601 aaacagataa agtgctgtac cgtcgggcaa aacagcaggt taagtacgag ttgactggac
     9661 aagcttatgg tttcgaatgg gacgtatctt accaatatat aggtcaacga tacgacaacg
     9721 cttatgacga gacctctggc tcatcacata cagttaaatt aggcggtgtg agcttgtggg
     9781 atcttgcggt tgcgtatccg gtcacctctc atctaacagt tcgtggtaaa atagccaacc
     9841 tgttcgacaa agattatgag acagtctatg gctaccaaac tgcaggacgg gaatacacct
     9901 tgtctggcag ctacaccttc tgaaccacgt cccaccgtgc tggtgtttga ctccggcgtc
     9961 ggtgggttgt cggtctatga cgagatccgg catctcttac cggatctcca ttacatttat
    10021 gctttcgata acgtcgcttt cccgtatggc gaaaaaagcg aagagtttat tgttgagcga
    10081 gtggtggcaa ttgtcaccgc agtgcaagaa cgttatcccc ttgcgctggc tgtggtcgct
    10141 tgcaacaccg ccagtaccgt ttcacttcct gcattacgcg aaaagttcga cttcccggtt
    10201 gttggtgtcg tgccggcgat taaacctgct gcacgtctga cggcaaatgg cattgtcgga
    10261 ttactggcaa cccgcggaac tgttaaacgt tcttatactc atgagctgat cgcgcgtttc
    10321 gctaatgaat gccagataga aatgctgggc tcggcagaga tggtcgagtt ggctgaagcg
    10381 aagctgcatg gcgaagatgt ttctctggat gcgctaaaac gtatcttgcg tccgtggtta
    10441 agaatgaaag agccgccaga taccgttgta ctggggtgta cccatttccc tctactacaa
    10501 gaagaactgt tacaggtgct gccagaggga acccggctgg tggattctgg cgcagcgatt
    10561 gctcgccgaa cggcctggct gttagaacat gaagccccgg atgcaaaatc tgccgatgcg
    10621 aatattgcct tttgtatggc aatgacgcca gaagctgaac aattattgcc cgttttacag
    10681 cgttacggct tcgaaacgct cgaaaaactg gcagttttag gctgatttgg ttgaatgttg
    10741 cgcggtcaga aaattatttt aaatttcctc ttgtcaggcc ggaataactc cctataatgc
    10801 gccaccactg acacggaaca acggcaaaca cgccgccggg tcagcggggt tctcctgaga
    10861 atctcaacag agaaaagcaa aaaaatgctt gactctgtag cgggaaagcg tattatgcac
    10921 accccgcgcc gctgagaaaa agcgaagcgg cactgctctt taacaattta tcagacaatc
    10981 tgtgtgggca ctcgaagata cggattctta acgtcgcaag acgaaaaatg aataccaagt
    11041 ctcaagagtg aacacgtaat tcattacgaa gtttaattct ttgagcatca aacttttaaa
    11101 ttgaagagtt tgatcatggc tcagattgaa cgctggcggc aggcctaaca catgcaagtc
    11161 gaacggtaac aggaagaagc ttgcttctt
//
LOCUS       DAZUCC010000167        11141 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZUCC010000167 DAZUCC010000000
VERSION     DAZUCC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.168"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..170
                     /locus_tag="ACHY09_003764"
                     /pseudo
     CDS             1..170
                     /locus_tag="ACHY09_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(195..509)
                     /gene="fliE"
                     /locus_tag="ACHY09_003765"
     CDS             complement(195..509)
                     /gene="fliE"
                     /locus_tag="ACHY09_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFS3886675.1"
                     /translation="MSAIQGIEGVISQLQATAMSARTQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            724..2382
                     /gene="fliF"
                     /locus_tag="ACHY09_003766"
     CDS             724..2382
                     /gene="fliF"
                     /locus_tag="ACHY09_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFS3886676.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2375..3370
                     /gene="fliG"
                     /locus_tag="ACHY09_003767"
     CDS             2375..3370
                     /gene="fliG"
                     /locus_tag="ACHY09_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFS3886677.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3363..4049
                     /gene="fliH"
                     /locus_tag="ACHY09_003768"
     CDS             3363..4049
                     /gene="fliH"
                     /locus_tag="ACHY09_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFS3886678.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGVAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4049..5422
                     /gene="fliI"
                     /locus_tag="ACHY09_003769"
     CDS             4049..5422
                     /gene="fliI"
                     /locus_tag="ACHY09_003769"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFS3886679.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGSALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5441..5884
                     /gene="fliJ"
                     /locus_tag="ACHY09_003770"
     CDS             5441..5884
                     /gene="fliJ"
                     /locus_tag="ACHY09_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFS3886680.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            5881..7008
                     /gene="fliK"
                     /locus_tag="ACHY09_003771"
     CDS             5881..7008
                     /gene="fliK"
                     /locus_tag="ACHY09_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFS3886681.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETPPVINDKQSTSTSLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSSVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7113..7577
                     /gene="fliL"
                     /locus_tag="ACHY09_003772"
     CDS             7113..7577
                     /gene="fliL"
                     /locus_tag="ACHY09_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFS3886682.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7582..8586
                     /gene="fliM"
                     /locus_tag="ACHY09_003773"
     CDS             7582..8586
                     /gene="fliM"
                     /locus_tag="ACHY09_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFS3886683.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8583..8996
                     /gene="fliN"
                     /locus_tag="ACHY09_003774"
     CDS             8583..8996
                     /gene="fliN"
                     /locus_tag="ACHY09_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFS3886684.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            8999..9364
                     /gene="fliO"
                     /locus_tag="ACHY09_003775"
     CDS             8999..9364
                     /gene="fliO"
                     /locus_tag="ACHY09_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFS3886685.1"
                     /translation="MNNHATVQSSAPVSTAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9364..10101
                     /gene="fliP"
                     /locus_tag="ACHY09_003776"
     CDS             9364..10101
                     /gene="fliP"
                     /locus_tag="ACHY09_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFS3886686.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLTGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10111..10380
                     /gene="fliQ"
                     /locus_tag="ACHY09_003777"
     CDS             10111..10380
                     /gene="fliQ"
                     /locus_tag="ACHY09_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFS3886687.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10389..>11141
                     /gene="fliR"
                     /locus_tag="ACHY09_003778"
     CDS             10389..>11141
                     /gene="fliR"
                     /locus_tag="ACHY09_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFS3886688.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLL"
BASE COUNT         2631 a         2966 c         3057 g         2487 t
ORIGIN      
        1 tcgtaacaac atccgacgtg gtgatatgtc aaatgagaca ctctgatggt caaagggttt
       61 atcgcgttta cagatcgtgg atggcagaaa actttcagaa tcaggtttcc attttaaacc
      121 agaaattaag tgagtttgtc ccatccatgc cccacgcggt gagatcggat gtaatcaaca
      181 acaggttaca aaacctacac ctgcatgctc atcacttcct gatacgccgc caccagctta
      241 ttacgcacct gaatccccat ttgcattgaa actgaggctt tttgcatatc ggtcatcaca
      301 tcgtttaacg ccacgccggg ttcaccgaga gtgaattttt ctgcctgcgt gcgggcggct
      361 gtttgtgtat cgcttatgcg atcgagcgcg gcgtgcagct gcccggcaaa actaatggtc
      421 ggttgtggca gtgattcctg tgtacgcgca ctcatcgccg tagcctgtaa ctggctgata
      481 accccttcaa tcccctgtat cgctgacatt ttcatctccc ggataatttc tggcagcaaa
      541 gcctaccagt aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac gggtttattg
      601 gcggatagaa aaaaacgaaa gcacaaataa tgggagcgtc aatttttcga gtttgctgac
      661 ccgggagtga gtcttgttcc actttgccaa taacgccgtc cataatcagc cacgaggtgc
      721 gcgatgaatg cgactgcagc ccagacaaaa tctcttgagt ggcttaatcg cctgcgtgcg
      781 aatccgaaaa ttccattgat tgttgccggc tccgcagcag tggcggtcat ggtcgcactg
      841 atcctgtggg cgaaagcccc cgactaccgc acattattca gcaatctttc cgatcaggat
      901 ggtggcgcaa ttgtcagcca actgacgcaa atgaatattc cttaccgctt cagcgaagcc
      961 agcggcgcta ttgaagttcc ggcagataaa gttcacgaac tgcgcctgcg cctggcgcaa
     1021 caaggtttgc ccaaaggcgg cgcggtcggt ttcgaactgc tcgatcagga aaagtttggt
     1081 atcagccagt tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga gctttctcgt
     1141 accatcgaaa ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg
     1201 tctttattcg tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc
     1261 ggccgcgcac tcgatgaagg gcaaattagc gccattgtac atctggtttc cagcgcggtt
     1321 gctggtctgc cgccggggaa cgtcacgctg gtggatcagg gtggacatct gttaacccag
     1381 tccaatacca gcgggcgcga tcttaatgac gctcagttga aatatgccag cgatgtcgaa
     1441 ggccggattc agcggcgtat tgaagcgatc ctgtcgccaa ttgttggtaa cggtaatatt
     1501 cacgcccagg tcacggcgca actggacttc gccagtaaag aacaaacaga agaacagtat
     1561 cgccctaacg gtgatgaatc tcaggcggcg cttcgttcac gccagcttaa tgagagcgag
     1621 caaagcggtt ccggttatcc aggcggcgta ccgggggcgt tgtcgaatca accggcacct
     1681 gcgaataacg cgccaatcag cacgcctccg gcaaatcaaa ataaccgcca gcagcaggcg
     1741 agcaccacca gcaatagtgg gccgcgtagc acacagcgta atgaaaccag taactacgaa
     1801 gtcgatcgca ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc
     1861 gcggtagtgg tgaattacaa aaccttgcca gacggtaaac cgttgcctct cagcaacgaa
     1921 cagatgaagc aaattgaaga tctgacccgc gaggcgatgg gcttttctga aaaacgcggt
     1981 gactcgctca atgtcgttaa ctcgccgttc aatagtagtg aggaaagcgg cggagaactg
     2041 ccgttctggc aacaacaagc gtttatcgat cagttacttg ctgccggtcg ctggttgctg
     2101 gtactgctgg tggcgtggct gctgtggcgt aaagcggtac gcccgcagct aacacgtcgc
     2161 gctgaggcga tgaaagctgt acagcaacag gcgcaggccc gcgaggaagt ggaagatgcg
     2221 gtggaagtcc gcctgagcaa agacgaacaa ctccaacaac ggcgcgctaa ccaacgtctg
     2281 ggggcagaag tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg
     2341 gcgctggtca ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa
     2401 aagcgtcatc ctgctgatga ccattggcga agaccgggcg gcagaggtgt tcaagcacct
     2461 ctcccagcgc gaagtgcaaa ctctgagcgc tgcaatggcg aacgtcacgc agatctccaa
     2521 caagcagcta accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgccgcact
     2581 gaatatcaac gccaacgatt atctgcgttc ggtattggtc aaagctctgg gtgaagaacg
     2641 tgccgccagc ctgctggagg atattctcga aacccgcgat accgccagcg gtattgaaac
     2701 gctcaacttt atggagccgc agagcgccgc cgatctgatt cgcgatgagc atccgcaaat
     2761 tatcgccacc attctggtgc atctgaagcg cgcccaggcc gccgatattc tggcgttgtt
     2821 cgatgaacgt ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc
     2881 agccgcgctg gcggaactga ccgaagtact gaatggcttg ctcgacggtc agaatctcaa
     2941 gcgcagcaaa atgggcggcg tgagaacggc agccgaaatt atcaacctga tgaaaacgca
     3001 gcaggaagaa gccgttatta ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat
     3061 catcgatgag atgttcctgt tcgagaatct ggtggatgtc gatgatcgca gcattcagcg
     3121 tctgttgcag gaagtggatt ccgaatcgct gctgatcgcg ctgaaagggg ccgagcagcc
     3181 actgcgcgag aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga
     3241 tctcgccaac cgtggtccgg tgcgtctgtc gcaggtggaa aacgaacaga aagcgattct
     3301 gctgattgtg cgccgccttg ccgaaaccgg cgagatggtg attggcagcg gcgaggatac
     3361 ctatgtctga taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccacagg
     3421 cagagtttgt gccaatggtc gagtcggaag aaaccattat tgaagaggcc gaacccagcc
     3481 ttgagcagca actggcgcaa ctgcaaatgc aggcccatga gcaaggttat caggcgggtg
     3541 ttgccgaagg tcgccagcaa ggtcataagc agggctatca ggaaggactg gcccaggggc
     3601 tggaacaagg tctggcagag gcgaagtctc aacaagcgcc aattcatgcc cggatgcagc
     3661 aactggtcag cgaatttcaa actacccttg atgcgcttga tagtgtgatt gcttcgcgcc
     3721 tgatgcagat ggcgctggag gcggcacgtc aggtgatcgg tcagacgcca acggtggata
     3781 actcggcact gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggta
     3841 aaccacagct gcgcgtgcac ccggatgatc tgcaacgtgt tgatgatatg ctcggcgcaa
     3901 ctttaagttt gcatggctgg cgcttgcggg gcgatcccac cctccatcct ggcggctgta
     3961 aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct
     4021 gccgtctggc agcaccagga gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac
     4081 gctggataac tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt
     4141 aacccgcgct accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac
     4201 ctgtgtcatt gagcgccaga acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg
     4261 ctttaacggt caacggctgt ttttaatgcc gctggaggaa gtcgaaggtg tcctgcccgg
     4321 cgcgcgtgtt tatgccaaaa acatttcggc tgaagggctg caaagcggca agcagttgcc
     4381 gctcggttcg gcgttattag gtcgcgttct ggacggcagc ggtaaaccgc ttgatggcct
     4441 cccctccccc gatacgacgg aaaccggtgc gctgattacc ccgccattta acccgttgca
     4501 acgtacaccg attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac
     4561 cgtcgggcgc gggcagcgta tggggctgtt tgccgggtcc ggcgttggta aaagtgtgct
     4621 gctggggatg atggcacgtt acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga
     4681 acgagggcgc gaagtaaaag attttattga gaatatcctc ggtgccgaag gacgtgcacg
     4741 ctccgtggtg attgccgctc cggcggatgt ttctccgctc ctgcgaatgc agggtgccgc
     4801 ctatgccacg cgcattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat
     4861 ggactccctc acccgctacg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc
     4921 acctgccact aaaggttatc caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg
     4981 tgccggaaat ggcattagcg gcggcggctc gattaccgcg ttttataccg tgctcaccga
     5041 aggcgatgac cagcaggacc ccattgccga ctccgcgcgg gcaatcctcg acggtcacat
     5101 tgtgctgtct cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc
     5161 gatcagtcgc gcaatgacgg cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt
     5221 caaacagctg ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc
     5281 caaaggcagc gatccgatgc tcgacaaagc catcgccctg tggccgcagc tggagggcta
     5341 tttgcaacaa ggcatttttg aacgcgcgga ctgggaagcg tctctccagg ggctggagcg
     5401 tattttcccg acagtatcat aacccaggag ataacggcag atgacagaac atggcgcgct
     5461 ggcgaccctg aaagatctgg cagaaaaaga ggtagaggat gccgcgcgcc tgctgggtga
     5521 aatgcgtcgg ggatgtcagc aggcggaaga acagctcaaa atgctgatcg attatcagaa
     5581 tgaatatcgc aataacctca atagcgatat gagtgccggg atgaccagca accgctggat
     5641 caactatcag cagtttatcc agacgctgga aaaagccatt actcagcatc gccagcaact
     5701 taatcagtgg acgcagaaag tagacattgc cctgaacagc tggcgagaaa agaaacaacg
     5761 tttgcaggct tggcagacac tgcaggaacg gcaatccacg gcggcactgc ttgcagaaaa
     5821 ccgcctcgat cagaaaaaga tggatgagtt cgcccagcgc gccgccatga ggaaacctga
     5881 atgattcgca tagcgccctt gattaccgct gacgttgata acgccacatt gcctggcggc
     5941 aaagccagcg atgctgcaca agattttctc gcgttgttga gcgaagcatt agcaggcgag
     6001 acaacaaccg acaaagcggc cccccagttg ctggtggcaa cagataagcc cacgacaaaa
     6061 ggcgagccgc tggtcagcga tattgtttcc gatgcgcaac aagcagattt actgatccct
     6121 gtggatgaaa caccgcctgt catcaacgac aaacaatcca catcaacatc gttaaccacc
     6181 gctcagacga taacgttggc tgcggcggct gacaacaata cggcaaaaga cgaaaaagcg
     6241 gatgatctga atgaagacgt caccgcaagc ctgagcgccc tttttgcgat gttgccgggt
     6301 tttgacaata cgcccaaagt gactgatgca ccgtcaaccg tgttaccgac agagaaacca
     6361 acgcttttca caaaactgac ttctgcgcaa ctcactacag cacagcctga tgacgccccc
     6421 ggcacaccag ctcagccatt aacaccgctg gtagcagaag cccagagtaa agcggaagtc
     6481 atcagcacgc cttcgtcggt gaccgctgcc gccagcccgc taatcactcc acaccagaca
     6541 cagccactgc ccaccgtcgc cgcgcctgtt ttgagtgcac cgctgggttc tcacgaatgg
     6601 caacaatcat taagccagca tatttcgctg ttcacccgcc aggggcaaca aagtgcagag
     6661 ttgcgtctgc acccacagga tttaggtgaa gtgcaaattt ccctcaaagt ggatgataac
     6721 caggcgcaaa tccagatggt ttcaccgcat cagcacgtac gcgccgccct ggaagcagcg
     6781 ctgccggtac tgcgtacgca gctggccgaa agtggcattc agttagggca aagcaacatc
     6841 agtggcgaaa gctttagtgg tcagcagcag gccgcttccc aacaacagca aagccaacgc
     6901 acagcaaacc atgaacctct ggcgggggaa gacgacgata cgcttccggt tcccgtctct
     6961 ttacaagggc gtgtaacagg caacagcggc gttgatattt tcgcctaacg tcagaggtag
     7021 caccgtaatc cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat aattagccga
     7081 taagcagtag cgacacagga agaccgcaac acatgactga ttacgcgata agcaagaaaa
     7141 gcaagcgatc gctttggatc ccgattctgg tattcattac cctcgcggcc tgtgccagcg
     7201 caggttacag ctactggcat tcgcatcagg ttgccgctga agacaaagcg cagcaacgcg
     7261 tcgtgccctc cccggtcttc tatgcgctgg atacctttac ggtcaatctg ggcgatgcgg
     7321 atcgcgtact ttatatcggc ataaccctgc gactcaaaga tgaagctacc cgctcgcggc
     7381 tgagtgagta tttgccggaa gtccgtagcc gtttgctgtt actgttttcg cgtcaggatg
     7441 ctgccgtact ggcgacagaa gaaggtaaga aaaacctgat tgccgagatt aaaaccacac
     7501 tttccacccc gcttgttgcc gggcaaccga aacaggatgt caccgacgtg ctgtataccg
     7561 cttttattct gcgataacga catgggcgat agtattcttt ctcaagctga aattgatgcg
     7621 ctgttgaatg gtgacagcga agtcaaagac gaaccgacag ccagtgttag cggcgaaagt
     7681 gacattcgtc cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg tttgcaggcg
     7741 ctggaaatca ttaatgagcg ctttgcccgc cattttcgta tggggctgtt caacctgctg
     7801 cgccgtagcc cggacattac cgtcggggcc atccgcattc agccgtacca tgaatttgcc
     7861 cgcaacttgc cggtgccgac caacctgaac cttatccacc ttaaaccgct gcgcggcaca
     7921 gggctggtgg tgttctcacc gagtctggtg tttatcgccg tggataacct gtttggcggc
     7981 gatggacgct tcccgaccaa agtggaaggt cgcgagttta cccatacaga acagcgcgtc
     8041 atcaaccgca tgttaaagct ggcgcttgaa ggctatagcg acgcctggaa ggcgattaat
     8101 ccgctggaag ttgagtacgt acgttcggaa atgcaggtga aatttaccaa tatcaccacc
     8161 tcaccgaacg acatcgtggt taacacgccg ttccatgtgg agattggcaa cctgaccggc
     8221 gaatttaata tctgtctgcc attcagcatg atcgagccgc tacgggaatt gttggttaac
     8281 ccgccgctgg aaaactcgcg taatgaagat cagaactggc gcgataacct ggtgcgccag
     8341 gtgcagcatt cacagctgga gctggtcgcc aactttgccg atatatcgct acgcctgtcg
     8401 cagattttaa aactgaaacc cggtgacgtc ctgccgatag aaaaacccga ccgcatcatc
     8461 gcccatgttg acggtgtccc ggtgctgacc agtcagtacg gcaccctcaa cggtcagtat
     8521 gcgttacgga tagaacattt gattaacccg attttaaatt ctctgaacga ggaacagccc
     8581 aaatgagtga catgaataat ccggccgatg acaacaacgg cgcaatggac gatctgtggg
     8641 ctgaagcgtt gagcgaacaa aaatcaacca gcggcaaaag cgctgccgac gcggtgttcc
     8701 agcaatttgg cggtggtgat gtcagcggaa cgttgcagga tatcgacctg attatggata
     8761 ttccggtcaa gctgaccgtc gagctgggcc gcacacgaat gaccatcaaa gagttattgc
     8821 gtctgacgca agggtccgtc gtggcgctgg acggcctggc gggcgaaccg ctggatattc
     8881 tgatcaacgg ttatttaatt gctcagggcg aagttgtcgt agtcgctgat aaatatggcg
     8941 tgcggatcac cgatatcatt actccgtctg agcgaatgcg tcgcctgagc cgttagtgat
     9001 gaataaccat gctactgtgc aatcttccgc gccggtttct actgcgccac tgctgcaggt
     9061 gagcggcgca ctcatcgcca ttattgccct gatcctcgct gctgcctggc tggtaaaacg
     9121 gctgggattt gccccgaaac gcactggcgt taacggtctg aaaattagcg ccagtgcttc
     9181 gctgggcgcg cgtgaacggg tcgtggtggt cgatgtggaa gatgcacggc tggtgctcgg
     9241 cgttaccgca gggcaaatca atctgctgca taaacttccc ccttcagcac caacggaaga
     9301 gataccgcag accgattttc agtcggtcat gaaaaacttg cttaagcgta gcgggagatc
     9361 ctgatgcgtc gtttgttgtc tgtcgcacct gtccttctct ggctggttac acccctcgcc
     9421 ttcgcgcaac tgacgggtat taccagccag ccgctgcctg gcggtggaca aagctggtcg
     9481 ctcccggtgc agacgctggt gttcattacc tcgttgacgt ttattccggc aattttactg
     9541 atgatgacca gtttcacccg catcatcatt gtttttggtt tattgcgtaa cgcactggga
     9601 acaccctccg cgccacctaa ccaggtattg ctggggctgg cactgttttt gacctttttt
     9661 attatgtcac cggtgatcga taaaatttat gtcgatgcgt accagccatt cagcgaagag
     9721 aaaatctcaa tgcaggaggc gctggaaaaa ggggcgcagc cgctgcgtga gtttatgctg
     9781 cgtcagaccc gtgaggcgga tttaggattg tttgccagac tggcaaatac tggcccgttg
     9841 cagggacctg aagccgtgcc gatgcgcatt ttgctcccgg cttacgtgac cagcgagttg
     9901 aaaaccgcat ttcagatagg cttcacgatc ttcattccgt ttttgattat cgacctggtg
     9961 attgccagcg tgttgatggc attggggatg atgatggtcc cccccgccac cattgctctg
    10021 ccctttaaac tgatgctgtt tgtactggtg gatggctggc aattgctggt cggttcgctg
    10081 gcgcagagct tttacagcta gagaggcaaa atgacacctg aatcggtcat gatgatgggg
    10141 actgaagcga tgaaagtcgc gctggcactg gctgccccgc tattgctggt agcattggtc
    10201 acgggcctta tcatcagtat tttgcaggcc gccacgcaga ttaacgaaat gacgctgtcg
    10261 tttattccga aaatcatcgc cgtatttatc gccattatta ttgccggacc gtggatgctc
    10321 aatctgttgc tggattacgt ccgcaccttg ttcactaacc tgccgtatat catcgggtag
    10381 gccgtacaat gatgcaggtg acaagcgatc aatggctttc ctggctaagt ctgtacttct
    10441 ggccgttact gcgcgtgttg gcgctgatct ccaccgcgcc gattctgagc gaacgcagcg
    10501 taccgaaacg ggtaaaactg ggtctggcaa tgatgatcac gttcgctatt gccccatcat
    10561 tacctgccaa cgatgttcct gttttttcgt tctttgctct gtggctggcc gtgcagcaga
    10621 tcctgatcgg cattgcgctt ggttttacca tgcaatttgc ctttgccgcg gtgcgaaccg
    10681 ctggtgaaat tatcggtctg caaatggggc tgtcatttgc gacgtttgtc gatccggcca
    10741 gccatcttaa tatgcccgtt ttagcgcgta tcatggatat gctggcgtta ctgctgttcc
    10801 tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt cacaccctgc
    10861 caattggtgg cgaaccgttg aacagcaatg cgtttctggc actcaccaaa gcagggagtt
    10921 tgattttcct taacgggctg atgctggcgt taccgctcat tactctgctg ctgaccctga
    10981 atctggcatt aggtttacta aatcgtatgg ccccgcaatt atccattttt gttattggat
    11041 ttccattaac tctgactgtc ggtatctctt taatggcggc attaatgccg ttaattgcgc
    11101 ctttttgcga acatttattc agtgagatgt ttaatttgct g
//
LOCUS       DAZUCC010000168        11138 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZUCC010000168 DAZUCC010000000
VERSION     DAZUCC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.169"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            478..858
                     /gene="atpI"
                     /locus_tag="ACHY09_003779"
     CDS             478..858
                     /gene="atpI"
                     /locus_tag="ACHY09_003779"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFS3886689.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            867..1682
                     /gene="atpB"
                     /locus_tag="ACHY09_003780"
     CDS             867..1682
                     /gene="atpB"
                     /locus_tag="ACHY09_003780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS3886690.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1729..1968
                     /gene="atpE"
                     /locus_tag="ACHY09_003781"
     CDS             1729..1968
                     /gene="atpE"
                     /locus_tag="ACHY09_003781"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFS3886691.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            2030..2500
                     /gene="atpF"
                     /locus_tag="ACHY09_003782"
     CDS             2030..2500
                     /gene="atpF"
                     /locus_tag="ACHY09_003782"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS3886692.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2515..3048
                     /gene="atpH"
                     /locus_tag="ACHY09_003783"
     CDS             2515..3048
                     /gene="atpH"
                     /locus_tag="ACHY09_003783"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS3886693.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            3061..4602
                     /gene="atpA"
                     /locus_tag="ACHY09_003784"
     CDS             3061..4602
                     /gene="atpA"
                     /locus_tag="ACHY09_003784"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS3886694.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4653..5516
                     /gene="atpG"
                     /locus_tag="ACHY09_003785"
     CDS             4653..5516
                     /gene="atpG"
                     /locus_tag="ACHY09_003785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS3886695.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5543..6925
                     /gene="atpD"
                     /locus_tag="ACHY09_003786"
     CDS             5543..6925
                     /gene="atpD"
                     /locus_tag="ACHY09_003786"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS3886696.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6946..7365
                     /gene="atpC"
                     /locus_tag="ACHY09_003787"
     CDS             6946..7365
                     /gene="atpC"
                     /locus_tag="ACHY09_003787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS3886697.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7714..9084
                     /gene="glmU"
                     /locus_tag="ACHY09_003788"
     CDS             7714..9084
                     /gene="glmU"
                     /locus_tag="ACHY09_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS3886698.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQSQKEGWR
                     RPVKKK"
     gene            9246..11075
                     /gene="glmS"
                     /locus_tag="ACHY09_003789"
     CDS             9246..11075
                     /gene="glmS"
                     /locus_tag="ACHY09_003789"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS3886699.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEYFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         2694 a         2741 c         3031 g         2672 t
ORIGIN      
        1 agaaaataag tcataagtga aaatatcagt ctgctaaaaa tcggcgctaa gaatcatcat
       61 tggctgttaa aacattatta aaaatgtcaa tgggtggttt ttgttgtgta aatgtcattt
      121 attaaaacag tatctgtttt tagactgaaa tatcataaac ttgcaaaggc atcatttgcc
      181 aagtaaataa atatgctgtg cgcgaacatg ctcaatatgt gatctgaagc acgctttatc
      241 accagtgttt acgcgttatt tacagttttt catgatcgaa cagggtttgc agaaaagtcg
      301 caattgtatg cactggaaaa atatttaaac atttattcac cttttggcta cttattgttt
      361 gaaatcacgg gggcgcaccg tataatttga ccgctttttg atgcttgact ctaagcctta
      421 aagaaagttt tatacgacac gcggcatacc tcgaagggag caggagtgaa aaacgtgatg
      481 tctgtgtcgc tcgtgagtcg aaacgttgct cggaagcttc tgctcgttca gttactggtg
      541 gtgatagcaa gtggattgct gttcagcctc aaagacccct tctggggcgt ctctgcaata
      601 agcgggggcc tggcagtttt tctgcctaac gttttgttta tgatatttgc ctggcgtcac
      661 caggcgcata caccagcgaa aggccgggtg gcctggacat tcgcatttgg cgaagctttc
      721 aaagttctgg cgatgttggt gttactggtg gtggcgttgg cggttttaaa ggcggtattc
      781 ttgccgctga tcgttacgtg ggttttggtg ctggtggttc agatactggc accggctgta
      841 attaacaaca aagggtaaaa ggcatcatgg cttcagaaaa tatgacgccg caggattaca
      901 taggacacca cctgaacaac cttcagctgg acctgcgtac attctcgctg gtggatccac
      961 ataacccccc agccaccttc tggacaatca atattgactc catgttcttc tcggtggtgc
     1021 tgggtctgtt gttcctggtt ttattccgta gcgtagccaa aaaggcgacc agcggtgtgc
     1081 caggtaagtt tcagaccgcg attgagctgg tgatcggctt tgttaatggt agcgtgaaag
     1141 acatgtacca tggcaaaagc aagctgattg ctccgctggc cctgacgatc ttcgtctggg
     1201 tattcctgat gaacttgatg gatttactgc ctatcgacct gctgccgtac attgctgaac
     1261 atgtactggg tctgcctgca ctgcgtgtgg ttccgtctgc ggacgtgaac gtaacgctgt
     1321 ctatggcact gggtgtattt atcctgattc tgttctacag catcaaaatg aaaggcatcg
     1381 gcggcttcac gaaagagttg acgctgcagc cgttcaatca ctgggcgttc attcctgtca
     1441 acttaatcct tgaaggggta agcctgctgt ccaaaccagt ttcactcggt ttgcgactgt
     1501 tcggtaacat gtatgccggt gagctgattt tcattctgat tgctggtctg ttgccgtggt
     1561 ggtcacagtg gattctgaat gtgccgtggg ccattttcca catcctgatc attacgctgc
     1621 aagccttcat cttcatggtt ctgacgatcg tctatctgtc gatggcgtct gaagaacatt
     1681 aatttaccaa cactactacg ttttaactga aacaaactgg agactgtcat ggaaaacctg
     1741 aatatggatc tgctgtacat ggctgccgct gtgatgatgg gtctggcggc aatcggtgct
     1801 gcgatcggta tcggcatcct cgggggtaaa ttcctggaag gcgcagcgcg tcaacctgat
     1861 ctgattcctc tgctgcgtac tcagttcttt atcgttatgg gtctggtgga tgctatcccg
     1921 atgatcgctg taggtctggg tctgtacgtg atgttcgctg tcgcgtagta agcgttgctt
     1981 ttatttaaag agcaatatca gaacgttaac taaatagagg cattgtgctg tgaatcttaa
     2041 cgcaacaatc ctcggccagg ccatcgcgtt tgtcctgttc gttctgttct gcatgaagta
     2101 cgtatggccg ccattaatgg cagccatcga aaaacgtcaa aaagaaattg ctgacggcct
     2161 tgcttccgca gaacgagcac ataaggacct tgaccttgca aaggccagcg cgaccgacca
     2221 gctgaaaaaa gcgaaagcgg aagcccaggt aatcatcgag caggctaaca aacgccgctc
     2281 gcagattctg gacgaagcga aagctgaggc agaacaggaa cgtactaaaa tcgtggccca
     2341 ggcgcaggcg gaaattgaag ccgagcgtaa acgtgcccgt gaagagctgc gtaagcaagt
     2401 tgctatcctg gctgttgctg gcgccgagaa gatcatcgaa cgttccgtgg atgaagctgc
     2461 taacagcgac atcgtggata aacttgtcgc tgaactgtaa ggagggaggg gctgatgtct
     2521 gaatttatta cggtagctcg cccctacgcc aaagcagctt ttgactttgc cgtcgaacac
     2581 caaagtgtag aacgctggca ggacatgctg gcgtttgccg ccgaggtaac caaaaacgaa
     2641 caaatggcag agcttctctc tggcgcgctt gcgccagaaa cgctcgccga gtcgtttatc
     2701 gcagtttgtg gtgagcaact ggacgaaaac ggtcagaacc tgattcgggt tatggctgaa
     2761 aatggtcgtc ttaacgcgct cccggatgtt ctggagcagt ttattcacct gcgtgccgtg
     2821 agtgaggcta ccgctgaggt agacgtcatt tccgctgccg cactgagtga acaacagctc
     2881 gcgaaaattt ctgctgcgat ggaaaaacgt ctgtcacgca aagttaagct gaattgcaaa
     2941 atcgataagt ctgtaatggc aggcgttatc atccgagcgg gtgatatggt cattgatggc
     3001 agcgtacgcg gtcgtcttga gcgccttgca gacgtcttgc agtcttaagg ggactggagc
     3061 atgcaactga attccaccga aatcagcgaa ctgatcaagc agcgcattgc tcagttcaat
     3121 gttgtgagtg aagctcacaa cgaaggtact attgtttctg taagtgacgg tgttatccgc
     3181 attcacggcc tggccgattg tatgcagggt gaaatgatct ccctgccggg taaccgttac
     3241 gctatcgcac tgaacctcga gcgcgactct gtaggtgcgg ttgttatggg tccgtacgct
     3301 gaccttgccg aaggcatgaa agttaagtgt actggccgta tcctggaagt tccggttggc
     3361 cgtggcctgc tgggccgtgt ggttaacact ctgggtgcac caatcgacgg taaaggtccg
     3421 ctggatcacg acggcttctc tgctgtagaa gcaatcgctc cgggcgttat cgaacgtcag
     3481 tccgtagatc agccggtaca gaccggttat aaagccgttg actccatgat cccaatcggt
     3541 cgtggtcagc gtgaattgat catcggtgac cgtcagaccg gtaaaaccgc actggctatc
     3601 gatgccatca tcaaccagcg cgattccggt atcaaatgta tctatgtcgc tatcggccag
     3661 aaagcgtcca ccatttctaa cgtggtacgt aaactggaag agcacggcgc actggctaac
     3721 accatcgttg tggtagcaac cgcgtctgaa tccgctgcac tgcaatacct ggcaccgtac
     3781 gctggttgtg caatgggcga atacttccgt gaccgcggtg aagatgcgct gatcatttac
     3841 gatgacctgt ctaaacaggc tgttgcttac cgtcagatct ccctgctgct ccgtcgtccg
     3901 ccaggacgtg aagcattccc gggcgacgta ttctacctcc actctcgtct gctggagcgt
     3961 gctgcgcgtg ttaacgccga atacgttgaa gccttcacca aaggtgaagt gaaagggaaa
     4021 accggttctc tgactgcgct gccgattatc gaaactcagg cgggtgacgt ttctgcgttc
     4081 gttccaacca acgtaatctc cattaccgat ggtcagatct tcctggaaac caacctgttc
     4141 aacgccggta ttcgtcctgc ggttaacccg ggtatttccg tatcccgtgt tggtggtgca
     4201 gcacagacca agatcatgaa aaaactgtcc ggtggtatcc gtaccgctct ggcacagtat
     4261 cgtgaactgg cagcgttctc tcagtttgca tccgaccttg acgatgcaac acgtaagcag
     4321 cttgaccacg gtcagaaagt gaccgaactg ctgaagcaga aacagtatgc gccgatgtct
     4381 gttgcgcaac agtctctggt tctgttcgca gcagaacgtg gttacctggc ggatgtagaa
     4441 ctgtcgaaaa tcggcagctt cgaagccgct ctgctggctt acgtcgaccg tgatcacgct
     4501 ccgttgatgc aagagatcaa ccagaccggt ggctacaacg acgaaatcga aggcaagctg
     4561 aaaggcatcc tcgattcctt caaagcaacc caatcctggt aacgtctggc ggcttgcctt
     4621 agggcaggcc gcaaggcatt gaggagaagc tcatggccgg cgcaaaagag atacgtagta
     4681 agatcgcaag cgtccagaac acgcaaaaga tcactaaagc gatggagatg gtcgccgctt
     4741 ccaaaatgcg taaatcgcag gatcgcatgg cggccagccg tccttatgca gaaaccatgc
     4801 gcaaagtgat tggtcacctt gcacacggta atctggaata taagcaccct tacctggaag
     4861 accgcgacgt taaacgcgtg ggctacctgg tggtgtcgac cgaccgtggt ttgtgcggtg
     4921 gtttgaacat taacctgttc aaaaaactgc tggcggaaat gaagacctgg accgacaaag
     4981 gcgttcaatg cgacctcgca atgatcggct cgaaaggcgt gtcgttcttc aactccgtgg
     5041 gcggcaatgt tgttgcccag gtcaccggca tgggggataa cccttccctg tccgaactga
     5101 tcggtccggt aaaagtgatg ttgcaggcct acgacgaagg ccgtctggac aagctttaca
     5161 ttgtcagcaa caaatttatt aacaccatgt ctcaggttcc gaccatcagc cagctgctgc
     5221 cgttaccggc atcagatgat gatgatctga aacataaatc ctgggattac ctgtacgaac
     5281 ccgatccgaa ggcgttgctg gataccctgc tgcgtcgtta tgtcgaatct caggtttatc
     5341 agggcgtggt tgaaaacctg gccagcgagc aggccgcccg tatggtggcg atgaaagccg
     5401 cgaccgacaa tggcggcagc ctgattaaag agctgcagtt ggtatacaac aaagctcgtc
     5461 aggccagcat tactcaggaa ctcaccgaga tcgtctcggg ggccgccgcg gtttaaacag
     5521 gttatttcgt agaggattta agatggctac tggaaagatt gtccaggtaa tcggcgccgt
     5581 agttgacgtc gaattccctc aggatgccgt accgcgcgtg tacgatgctc ttgaggtgca
     5641 aaatggtaat gagcgtctgg tgctggaagt tcagcagcag ctcggcggcg gtatcgtacg
     5701 taccatcgca atgggttcct ccgacggtct gcgtcgcggt ctggatgtaa aagacctcga
     5761 acacccgatc gaagtcccgg taggtaaagc gactctgggc cgtatcatga acgtactggg
     5821 tgaaccggtc gacatgaaag gcgagatcgg tgaagaagag cgttgggcga ttcaccgcgc
     5881 agcaccttcc tacgaagagc tgtcaaactc tcaggaactg ctggaaaccg gtatcaaagt
     5941 tatcgacctg atgtgtccgt tcgctaaggg cggtaaagtt ggtctgttcg gtggtgcggg
     6001 tgtaggtaaa accgtaaaca tgatggagct tattcgtaac atcgcgatcg agcactccgg
     6061 ttactctgtg tttgcgggcg taggtgaacg tactcgtgag ggtaacgact tctaccacga
     6121 aatgaccgac tccaacgtta tcgacaaagt atccctggtg tatggccaga tgaacgagcc
     6181 gccgggaaac cgtctgcgcg ttgctctgac cggtctgacc atggctgaga aattccgtga
     6241 cgaaggtcgt gacgttctgc tgttcgttga caacatctat cgttacaccc tggccggtac
     6301 ggaagtatcc gcactgctgg gccgtatgcc ttcagcggta ggttatcagc cgaccctggc
     6361 ggaagagatg ggcgttctgc aggaacgtat cacctccacc aaaaccggtt ctatcacctc
     6421 cgtacaggcg gtatacgtac ctgcggatga cttgactgac ccgtctccgg caaccacctt
     6481 tgcgcacctt gacgcaaccg tggtactgag ccgtcagatc gcgtctctgg gtatctaccc
     6541 ggccgttgac ccgctggact ccaccagccg tcagctggac ccgctggtgg ttggtcagga
     6601 acactacgac accgcgcgtg gcgttcagtc catcctgcaa cgttatcagg aactgaaaga
     6661 cattatcgcc atcctgggta tggatgaact gtctgaagaa gacaaactgg tggtagcgcg
     6721 tgctcgtaag atccagcgct tcctgtccca gccgttcttc gtggcagaag tattcaccgg
     6781 ttctccgggt aaatacgtct ccctgaaaga caccatccgt ggctttaaag gcatcatgga
     6841 aggcgaatac gatcacctgc cggagcaggc gttctacatg gtcggttcca tcgaagaagc
     6901 tgtggaaaaa gccaaaaaac tttaacgcct taatcggagg gtgatatggc aatgacttac
     6961 cacctggacg tcgtcagtgc agagcaacaa atgttctctg gtctggtcga gaaaatccag
     7021 gtaacgggta gcgaaggtga actggggatc tatcctggcc acgcaccgct gctcaccgcc
     7081 attaagcctg gtatgattcg catcgtgaaa cagcacggtc acgaagagtt tatctatctg
     7141 tctggcggca ttcttgaagt gcagcctggc aacgtgaccg ttctggccga caccgcaatt
     7201 cgcggccagg atctcgacga agcgcgagcc atggaagcga aacgtaaggc tgaagagcac
     7261 attagcagct ctcacggcga cgtagattac gctcaggcgt ctgcggaact ggccaaagcg
     7321 atcgcgcagc tgcgcgttat cgagttgacc aaaaaagcga tgtaacaccg gcttgaaaag
     7381 cacaaaagcc agtctggaaa caggctggct ttttttacgc gtgacccgtc ctgaagaacg
     7441 ttcacataga tcctgctgat ataaaacccc ctgttttccc gtttattcat tgatcgaaat
     7501 aagagcaaaa acatccacct gacgcttaaa tgaaggtact gccttaattt tctgcagaca
     7561 aaaggtgtga cgatggtcga aaatggcgct ttcgtcagcg gggataatcc gttattgaac
     7621 aatttatcct ctgtccattt cacgatgaaa aaaatgtagt tttttcaagg tgaagcggtt
     7681 taaattcgtt ctcaaattac agtcaggacg cgtatgttga ataatgctat gagcgtagtg
     7741 atccttgccg caggcaaagg cacgcgcatg tattccgatc ttccgaaagt gctgcatacc
     7801 cttgccggga aagcgatggt tcagcatgtc attgatgctg cgaatgaatt aggcgcagcg
     7861 cacgttcacc tggtttacgg tcacggcggc gatctgctta aacaggcgct gaaagatgac
     7921 aacctgaact gggtgcttca ggcagaacag ctgggtacag gtcatgcgat gcagcaggcc
     7981 gcacctttct ttgccgatga tgaagacatt ttaatgctct acggcgacgt gccgctgatc
     8041 tctgtcgaaa cactccagcg tctgcgtgat gctaaaccgc agggtggcat tggtctgctg
     8101 acggtaaaac tggatgatcc gaccggttat ggacgtatca cccgtgaaaa cggcaaagtt
     8161 accggcattg ttgagcacaa agacgccacc gacgagcagc gtcagattca ggagatcaac
     8221 accggcattc tgattgccaa cggcgcagat atgaaacgct ggctggcgaa gctgaccaac
     8281 aataacgctc agggtgaata ctacatcacc gacattattg cgctggcgta tcaggaaggg
     8341 cgtgaaatcg tcgccgttca tccgcaacgt ttaagcgaag tagaaggcgt gaataaccgc
     8401 ctgcaactct cccgactgga gcgcgtttac cagtctgaac aggctgaaaa actgctgtta
     8461 gcaggcgtta tgctgcgcga tccggcgcgt tttgatctgc gcggtacgct tactcacggg
     8521 cgcgatgttg aaattgatac taacgttatc atcgagggca acgtgactct cggtcatcgc
     8581 gtgaaaatcg gcaccggttg cgtgattaaa aacagcgtga ttggcgatga ttgcgaaatc
     8641 agtccgtata ccgttgtgga agatgcgaat ctggcagcgg cctgtaccat tggcccgttt
     8701 gcccgtctgc gtcctggtgc tgagttgctg gaaggtgcac acgtcggtaa cttcgttgag
     8761 atgaaaaaag cgcgtctggg taaaggctcg aaagccgggc atctgactta tctgggcgat
     8821 gcggaaattg gcgataacgt taacatcggc gcgggaacca ttacctgcaa ctacgacggt
     8881 gcgaataaat ttaagaccat tatcggcgac gatgtgtttg ttggttccga tactcagctg
     8941 gtggccccgg taacggtagg caaaggcgca accattgctg cgggtacaac tgtgacgcgt
     9001 aatgtcggcg aaaatgctct ggcaatcagc cgtgtgccgc agtcgcaaaa agaaggctgg
     9061 cgtcgtccgg taaagaaaaa gtaattctgg ctgggaaatc cggtcatatg ggatgaggag
     9121 ataacataat ctcccgccca caagcagtaa ctataaaaat aaccccactc tctacaaggc
     9181 tcggggcgcc cgaaaaaacg ggcatacagg ttgaccgaca acgatataaa tcggaatcaa
     9241 aaactatgtg tggaattgtt ggcgcgatcg cgcaacgtga tgtagcagaa atccttcttg
     9301 aaggtttacg tcgtctggaa taccgcggat atgactctgc cggtctggcc gttgttgatg
     9361 cagaaggtca tatgacccgc ctgcgtcgcc tcggtaaagt acagatgctg gctcaggcag
     9421 cggaagaaca tcctctgcat ggcggcaccg gtattgctca tactcgctgg gcgacccacg
     9481 gtgaaccttc agaagctaac gcgcatccgc atgtttctga acacattgtg gtggtgcata
     9541 acggcatcat cgaaaaccat gaaccgctgc gtgaagagtt aaaagcgcgt ggctatacct
     9601 tcgtttctga aaccgacacc gaagtgattg cacatttggt gaactgggag ctgaaacaag
     9661 gcggtactct gcgtgaggca gttctgcgtg ctattccgca actgcgtggt gcgtacggta
     9721 cagtgatcat ggactcccgt cacccggata ccctgctggc ggcacgttct ggtagcccgc
     9781 tggtgattgg tctgggcatg ggcgaaaact ttatcgcttc tgaccagctg gcgctgttgc
     9841 cagtgactcg tcgctttatc ttccttgaag agggcgatat tgcggaaatc actcgccgtt
     9901 cggtaaacat tttcgataaa actggcgcgg aagtaaaacg tcaggatatt gaatccaatc
     9961 tgcaatatga cgcgggcgat aaaggtattt accgtcacta catgcagaaa gagatctacg
    10021 aacaaccgaa cgcgatcaaa aacaccctta ccgggcgcat cagccacggt caggttgatt
    10081 taagcgagct gggaccgaac gccgacgaac tgctgtcgaa ggttgagcat attcagatcc
    10141 tcgcctgtgg tacttcttat aactccggta tggtttcccg ctactggttt gaatcgctgg
    10201 caggtattcc gtgcgacgtc gaaatcgcct ctgaattccg ctatcgcaaa tctgccgtgc
    10261 gtcgtaacag cctgatgatc accttgtcac agtctggcga aaccgcggat accctggcgg
    10321 gcctgcgtct gtcgaaagag ctgggttacc ttggttcgct ggcaatctgt aacgttccgg
    10381 gttcttctct ggtgcgcgaa tccgatctgg cgctgatgac caacgccggt acggaaatcg
    10441 gcgtggcatc cactaaagca ttcaccactc agttaactgt gctgttgatg ctggtggcga
    10501 agctgtctcg cctgaaaggt ctggatgcct ccattgaaca tgacattgtg catggtctgc
    10561 aggcgctgcc gagccgtatt gagcagatgc tgtctcagga caaacgcatt gaagcgctgg
    10621 cagaatattt ctctgacaaa catcacgcgc tgttcctggg ccgtggcgat cagtacccaa
    10681 tcgcgctgga aggtgcattg aagctgaaag agatctctta cattcacgct gaagcctacg
    10741 ctgcgggcga actgaaacac ggcccgctgg cgctgattga cgccgatatg ccggttatcg
    10801 tcgttgcgcc gaacaacgag ttgttggaaa aactgaaatc taacattgaa gaagttcgcg
    10861 cgcgtggcgg tcagttgtat gtcttcgccg atcaggatgc gggttttgtg agcagtgaca
    10921 acatgcacat cattgagatg ccgcatgtgg aagaggtgat tgcaccaatc ttctacaccg
    10981 ttccgctgca gctactggct taccacgttg cgctgatcaa aggcaccgac gttgaccagc
    11041 cgcgtaacct ggcgaaatcg gttacggttg agtaatatct ctcgttgata gaaaagggct
    11101 gccgaccggc ggcccttttt tataagtgat atcaataa
//
LOCUS       DAZUCC010000169        11127 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZUCC010000169 DAZUCC010000000
VERSION     DAZUCC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.170"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            18..317
                     /gene="yoaC"
                     /locus_tag="ACHY09_003790"
     CDS             18..317
                     /gene="yoaC"
                     /locus_tag="ACHY09_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFS3886700.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(437..616)
                     /gene="yoaH"
                     /locus_tag="ACHY09_003791"
     CDS             complement(437..616)
                     /gene="yoaH"
                     /locus_tag="ACHY09_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFS3886701.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HAGERIVARFEDEDE"
     gene            690..2051
                     /gene="pabB"
                     /locus_tag="ACHY09_003792"
     CDS             690..2051
                     /gene="pabB"
                     /locus_tag="ACHY09_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFS3886702.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSENEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEGFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHTSDLLRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2054..2632
                     /gene="nudL"
                     /locus_tag="ACHY09_003793"
     CDS             2054..2632
                     /gene="nudL"
                     /locus_tag="ACHY09_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFS3886703.1"
                     /translation="MEHRSLTLDDFLSRFQLLRPQMNRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2816..4180
                     /gene="sdaA"
                     /locus_tag="ACHY09_003794"
     CDS             2816..4180
                     /gene="sdaA"
                     /locus_tag="ACHY09_003794"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFS3886704.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4311..5909
                     /gene="pdeD"
                     /locus_tag="ACHY09_003795"
     CDS             4311..5909
                     /gene="pdeD"
                     /locus_tag="ACHY09_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS3886705.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDHALIKGSPVLIQWTPAAGSSK
                     AGVMEMINIDLLAAMLLEPQLPQISSASLTVDDRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLLRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5913..7469)
                     /gene="yoaE"
                     /locus_tag="ACHY09_003796"
     CDS             complement(5913..7469)
                     /gene="yoaE"
                     /locus_tag="ACHY09_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFS3886706.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7457..7615)
                     /gene="yoaL"
                     /locus_tag="ACHY09_003797"
     CDS             complement(7457..7615)
                     /gene="yoaL"
                     /locus_tag="ACHY09_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFS3886707.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7932..8903
                     /gene="manX"
                     /locus_tag="ACHY09_003798"
     CDS             7932..8903
                     /gene="manX"
                     /locus_tag="ACHY09_003798"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFS3886708.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKATPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8966..9766
                     /gene="manY"
                     /locus_tag="ACHY09_003799"
     CDS             8966..9766
                     /gene="manY"
                     /locus_tag="ACHY09_003799"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFS3886709.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9779..10630
                     /gene="manZ"
                     /locus_tag="ACHY09_003800"
     CDS             9779..10630
                     /gene="manZ"
                     /locus_tag="ACHY09_003800"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFS3886710.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10685..>11127
                     /gene="yobD"
                     /locus_tag="ACHY09_003801"
     CDS             10685..>11127
                     /gene="yobD"
                     /locus_tag="ACHY09_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFS3886711.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         2715 a         2823 c         2817 g         2772 t
ORIGIN      
        1 attttaagga taagaatatg cctgctgtaa tagataaagc cctggatttc attggtgcca
       61 tggatgtatc agcgccaaca ccaagttcga tgaatgaaag tacggcgaag ggaatcttta
      121 aatatttaaa agaactggga gtacccgcga gtgccgctga tattacggcg cgagctgacc
      181 aggaaggctg gaaccctggt ttcacggaaa aaatggttgg atgggcaaaa aaaatggagt
      241 ctggtgaacg ctctgtgatt aaaaatcctg aatacttttc aacatatatg caggaagaac
      301 taaaagcact ggtttgagtt aaatttacat cagcataaat gggtcaggac gcttttaatc
      361 acatataaaa agcgtccttt ttctcacaat caaataatca tcggcttcta taacgaaagc
      421 gatacggatt agtgtgttac tcgtcttcat cctcaaaacg cgccacgatt cgctcgccgg
      481 catggttggc acgcagctct tctgccacca acgcgattgc ctggccgctg ctcatccctt
      541 gagccatcag ttcctggatc cgctcgacag ctttttgctg ctgttcatgg gtgagtgaag
      601 gtaaacctgc aaacattgtt aactcctgct aaattgttgg cgctaattat ttcatgctac
      661 ccggcacata gccagtagag tcaggactga tgaagacgtt atctcccgct gtgattactt
      721 taccctggcg tcaggacgcc gctgaatttt atttctcacg cttaagccac ctgccgtggg
      781 cgatgctttt acactccggc tatgctgatc atccatatag ccgctttgat attgtggtcg
      841 ccgagccgat ttgcacttta accactttcg gtaaagaaac cgttgttagt gaaaacgaaa
      901 aacgcacaac gaccactgat gacccgctac aggtgctcca gcaggtgctg gatcgcgcag
      961 acattcgccc agcgcataac gaagatttgc catttcaggg cggcgcgctg gggttgtttg
     1021 gctacgatct gggccgccgt tttgagtcac tgccagaaat tgcgcagcaa gatatcgttc
     1081 tgccggatat ggcagtgggt atctacgact gggcgctggt tgttgaccac cagcgtcaaa
     1141 cagtttcttt gctgagtcat aatgatgtca atgctcgtcg ggcctggctg gaaagccagc
     1201 aattctcgcc gcaggaaggt ttcacgctca cttccgactg gcaatccaat atgacccgcg
     1261 agcagtacgg cgaaaaattt cgccaggtac aggaatatct gcacagcggt gattgctatc
     1321 aggtgaatct cgcccagcgt tttcatgcga cctattctgg cgatgaatgg caggcattcc
     1381 ttcagcttaa tcaggccaac cgcgcgccat ttagcgcttt tttacgtctt gaacagggtg
     1441 caattttaag cctttcgcca gagcggttta ttctttgtga taatagtgaa atccagaccc
     1501 gcccgattaa aggcacgcta ccacgcctgc ccgatcctca ggaagatagc aaacaagcag
     1561 aaaaactggc gaactcagcg aaagatcgtg ccgaaaatct gatgattgtc gatttaatgc
     1621 gtaatgatat cggtcgcgtt gccgtagccg gttcggtaaa agtaccagag ctgttcgtgg
     1681 tcgaaccctt ccctgccgtg catcatctgg tcagcaccat aacggcgcgg ctaccagaac
     1741 agttacacac cagcgatctg ctgcgcgcag cttttcctgg tggctcaata actggggctc
     1801 cgaaagtacg ggctatggaa attatcgacg aactggaacc gcaccgacgc aatgcctggt
     1861 gcggcagcat tggctatttg agcttttgcg gcaacatgga taccagcatt actatccgca
     1921 cgctaactgc cattaacgga caaatatact gctctgcggg cggtggaatt gtcgccgata
     1981 gccaggaaga agcggaatat caggaaactt ttgataaagt taataagatc ctacgtcaac
     2041 tggagaaata aacgtggaac accgtagcct gacgcttgat gattttttat cgcgctttca
     2101 acttttgcgt ccgcaaatga accgggaaac gttaaatcat cgtcaggctg ccgtgttaat
     2161 ccccatcgtc cgtcgaccgc aaccggggtt gttgctgact cagcgttcga ttcatctgcg
     2221 taaacacgct ggacaagtgg cattccctgg aggcgcagtc gatgacacgg acgcatcagt
     2281 tatcgccgcc gcgctgcgcg aagctgaaga agaggtcgct ataccgcctt ccgccgttga
     2341 agttatcggc gtgctgccgc ccgtcgatag cgtaactggc taccaggtaa ccccagtggt
     2401 cggcattatc ccgcccgatc tgccgtatcg cgccagtgaa gatgaagtct cggcggtgtt
     2461 tgaaatgccg ctcgcccagg cattacatct gggtcgttat catcctttag atatctaccg
     2521 ccgtggcgat tcacatcggg tatggctgtc ctggtacgaa cagtattttg tatggggaat
     2581 gaccgcaggc ataattcgtg agctggcgct gcaaattggt gtgaaaccct gactatactt
     2641 atctttacat ctacaaaaca ctacttgaga caatcatcgc aatattagtt aaatcgcggt
     2701 ttttgattag tttaattcat gtgaatagtt aagccagtcg ccgcgttccc tcttacacta
     2761 tgcgctgtta ttagttcgtt actggaagtc cagtcacctt gtcaggagta ttatcgtgat
     2821 tagtctattc gacatgttta aggtggggat tggtccctca tcttcccata ccgtagggcc
     2881 tatgaaggcg ggtaaacagt tcgtcgatga tctggtcgaa aaaggcttac tggatagcgt
     2941 tactcgcgtt gccgtggacg tttatggttc actgtcgctg acgggtaaag gccaccacac
     3001 cgatatcgcc attattatgg gtcttgcagg taacgaacct gccaccgtgg atatcgacag
     3061 tattcccggt tttattcgcg acgtagaaga gcgcgaacgt ctgctgctgg cacagggacg
     3121 gcatgaagtg gatttcccgc gcgacaacgg gatgcgtttt cataacggta accttccgct
     3181 gcatgaaaac ggtatgcaaa tccacgccta taacggcgat gaagtcgtct acagcaaaac
     3241 ttattattcc atcggcggcg gttttatcgt cgatgaagaa cactttggtc aggatgctgc
     3301 caacgaagtg agcgtgccgt atccgttcaa atctgccacc gaactgctcg cgtactgtaa
     3361 tgaaaccggc tattcgcttt ctggtctcgc tatgcaaaac gaactggcgc tgcacagcaa
     3421 gaaagagatc gacgagtatt tcgcgcatgt ctggcaaacc atgcaggcat gtatcgatcg
     3481 cgggatgaat accgaaggcg tactgccagg cccgttgcgc gtgccacgtc gtgcgtcggc
     3541 cctgcgccgg atgctggttt ccagcgataa actgtctaac gatccgatga atgtcattga
     3601 ctgggtaaac atgtttgcgc tggcagttaa cgaagaaaac gccgccggtg gccgtgtggt
     3661 aactgcgcca actaacggtg cctgcggtat cgttccggca gtgctggctt actatgacca
     3721 ctttattgaa tcagttagcc cagacatcta tacccgttac tttatggcag cgggcgcgat
     3781 tggtgcactg tataaaatga acgcgtctat ttccggtgcg gaagtcggtt gccagggcga
     3841 agtaggtgtt gcctgttcaa tggctgctgc gggccttgcc gagctgttgg gcggtagccc
     3901 ggaacaggtt tgtgtggcag cggaaattgg catggagcac aacctcggtc tgacctgcga
     3961 ccccgttgca gggcaagttc aggtgccgtg cattgaacgt aatgccattg cctctgtgaa
     4021 ggcgattaac gccgcacgga tggctctgcg ccgcaccagt gcaccgcgcg tctcgctgga
     4081 taaggtcatc gaaacgatgt acgaaaccgg taaggacatg aacgctaaat accgcgaaac
     4141 ctcacgcggt ggtctggcaa tcaaagtcca gtgtgactaa tacttcttac tcgcccatct
     4201 gcaacggatg ggcgaattta ttcccgcttt ctcgtctgct gtaattttcc ccactacact
     4261 tccactgttg cgtcaggcgt ttgtcgccat acgcttacag ggtggcccgc atgcaaaaag
     4321 cacaacggat cattaaaacc tatcgccgta atcgaatgat tgtttgtacg atttgcgcac
     4381 tcgttacgct cgcttcgacc ctgagcgtgc gatttatttc acagcgtaac ttaaatcaac
     4441 aacgggtagt acaattcgcc aatcacgctg tagaggaatt agataaagta ctgcttcccc
     4501 tacaggcagg tagcgaagtc ttgcttccgc tgattggtct gccctgctct gtcgcccatt
     4561 tgccattacg taaacaggcg gcaaaactcc aaactgtgcg atccattggc ctggtgcaag
     4621 acggcacact ttattgctcc agcatttttg gttatcgcaa tgtgcccgtc gtggacattc
     4681 tggctgaact tcctgcaccg caaccacttt tacgcctgac gaccgaccat gccctgatta
     4741 aaggcagtcc ggttttgatt caatggacgc cagcagcggg cagtagcaaa gctggggtca
     4801 tggagatgat taacatcgac ttactggcgg caatgctgct tgagccacaa ctgccgcaaa
     4861 tcagtagcgc cagcctgacg gtggacgatc ggcatttgct ctatggtaat gggctggtag
     4921 attcccttcc gcaacctgaa aacaatgaaa actatcaggt ttcttcgcaa cgctttcctt
     4981 ttaccattaa cgttaatggt ccgggggcta cggcgctggc atggcactat cttccaacac
     5041 aattaccgct ggcggtgctg ctaagtttac ttgtgggcta tatcgcctgg ctggcgaccg
     5101 cttaccggat gagtttttcc cgcgaaatca atctgggcct ggcgcaacat gagttcgaat
     5161 tgttctgcca gcctttgctt aatgcgcgca gccagcaatg tattggcgta gagattttgc
     5221 tgcgctggaa caatccgcgt cagggctgga tttcgccgga tgtgtttatt cctatcgcgg
     5281 aagaacatca tttaattgtg ccactgaccc gctatgtgat ggcagaaacc attcgtcagc
     5341 gccatgtttt cccgatgagt agtcagtttc atgttggcat taacgtcgca cccagccatt
     5401 ttcgccgtgg tgtgctgata aaagatctca atcagtactg gtttagcgct cacccgattc
     5461 agcaactgat cctcgaaatc accgaacgcg atgccttact ggatgttgat tatcggattg
     5521 cccgcgagct acatcgtaaa aacgtcaaac tggcgattga tgacttcggc accggcaata
     5581 gttcattttc ctggcttgaa acattacgtc ctgacgtgct gaaaattgat aagtcattta
     5641 ccgcagctat aggttctgac gcggttaact cgacggtgac cgatatcatc atcgcactgg
     5701 ggcaaagact gaatattgaa ctggtggcgg agggtgtgga aacgcaagaa caggcgaaat
     5761 atttgctccg tcatggcgtc catattttgc aagggtattt gtacgcacag ccgatgccac
     5821 tacgtgattt tcccaaatgg ctggcgggca gccaaccgcc gcccgcccgg cataatggac
     5881 atatcacacc cattatgccg ttacgttaac gcttactcat cttcatcgtg cgccggttgc
     5941 tctttaacaa tgcgaaccag atcaacacga taatcattgg cttcaatgat ggtgatatgc
     6001 agtggcccta catcaatcac atcgcccaca cggggaatgt gaccatttgc cgagatcacg
     6061 aggcccgcga ccgtcgcgat atcatcgtca tcggcaaggt gctcaacatc aagcgcctgc
     6121 tgcaaggcat gcaaatctgt accgcctttt accagccagc cgtcaccgtc ggtaatgatt
     6181 tccggcgttt cgtcagcgtc cgggaattca cccgcaatgg cttccagcac atccagcggc
     6241 gtgaccaagc cttgtaccac accaaactcg ttggtcacga taacaaagct cccgcgagca
     6301 cgacgcagca cgcccagcag gttgatcgga tcgagggttt ccgggacgat aatcgccgga
     6361 gacgccgaag caatcgccgc cacatcaacg ccctcttcca gcgccaccag cagttcttta
     6421 gcacgtacga ttccgatgat ttcatccagt tcaccgcgac ataccgggaa caggctgtgc
     6481 ggtgaggaga gcagctgctc gcggatttca tctaccccga gattagcgtc aacccagctt
     6541 atttcaccgc gcggcgtcat gatcccgcgc agagaacgcg acgccagcgt cagtacgccg
     6601 ttaatcatgt aacgttcttc ttcggcaaat gcaccttccg ggatcggcat cggcatcggg
     6661 ttatcggcat cgtgctgaac attggcctga cgtttcccgc ccatcaaacg caggatggca
     6721 tcggcagtac gcgctcgcag cggcaaagtc gactggtgac gaataaagtt gcgacgcgca
     6781 atctggttaa acacttcgat gatgatcgag aagccaatcg cggcatacag gtaacctttc
     6841 ggaatgtgga aaccgaaacc ttctgccacc agactcagac caatcattaa caggaagctc
     6901 agacagagca ccaccaccgt ggggtgctgg ttaacaaatc gcgtcagcgg tttcgatgcc
     6961 agcagcataa ctgccatcgc aatcactacc gccgccatca tcaccggcaa atggttaacc
     7021 atccctactg cagtaattac cgcatccaac gagaagacgg cgtcaaggat gacaatctgt
     7081 gtgacgacca cccagaaact ggcgtagcct ttaccgtggc cggaatcatg atcgcggttt
     7141 tccagccgtt catgcagttc ggttgttgct ttgaacagca agaatatgcc cccgaacaac
     7201 ataatcaggt cgcgtccgga gaaggagaaa tccatgacgg taaatagcgg tttggtcagc
     7261 gtgaccatcc atgaaatcag cgacagcagc cccagacgca taatcagcgc cagtgataat
     7321 cccagcaaac gcgctttatc gcgttgtttt ggcggcagtt tgtcagcaag aatggcaatg
     7381 aagaccaggt tatcgatacc cagcacaatt tcgagaacaa caagcgtgag tagccccgcc
     7441 caaattgagg ggtccattaa gaattccatg acaagctcct gcttaaggaa tagctattcg
     7501 acgccagaaa taatgcaggc gtaacgacaa aatgcaaacg aaaggtgcgg catagagtgc
     7561 cagaaaggca ggcgttaaaa ggcctgatgc tgaaatgacg tcggtgacga tccatactgc
     7621 gggctactgc cctatactcc atggttgtta aacgggagtt aaacatatca gagacgcctc
     7681 tgatttggca aagatttacc ttcctttgca aacgaatgtg acaaggatat tttacctttc
     7741 gaaatttctg ctaatcgaaa gttaaattac ggatcttcat cacataaaat aattttttcg
     7801 atatctaaaa taaatcgcga aacgcagggg tttttggttg tagcccttat ctgaatcgat
     7861 tcgattgtgg acgacgattc aaaaatacat ctggcacgtt gaggtgttaa cgataataaa
     7921 ggaggtagca agtgaccatt gctattgtta taggcacaca tggttgggct gcagagcagt
     7981 tgcttaaaac ggcagaaatg ctgttaggcg agcaggaaaa cgtcggctgg atcgatttcg
     8041 ttccaggtga aaatgccgaa acgctgattg aaaagtacaa cgctcagttg gcaaaactcg
     8101 acaccactaa aggcgtgctg tttctcgttg atacatgggg aggcagcccg ttcaatgctg
     8161 ccagccgcat tgtcgtcgac aaagagcatt atgaagtcat tgcaggcgtt aacattccaa
     8221 tgctcgtgga aacgttaatg gcccgtgatg atgacccaag ctttgatgaa ctggtggcgc
     8281 tggcagtaga aacaggtcgt gaaggcgtga aagcactgaa agccaaaccg gttgaaaaag
     8341 ccgcgccagc acccgctgcc gcagcaccaa aagcgactcc aactccggca aaaccaatgg
     8401 gaccaaacga ctacatggtt attggccttg cgcgtatcga cgaccgtctg atacacggtc
     8461 aggtcgccac ccgctggacc aaagaaacca atgtctcccg tattattgtt gttagtgatg
     8521 aagtggctgc ggataccgtt cgtaagacac tgctcaccca ggttgcacct ccgggcgtaa
     8581 cagcacacgt agttgatgtt gccaaaatga ttcgcgtcta caacaacccg aaatatgctg
     8641 gcgaacgcgt aatgctgtta tttaccaacc caacagatgt agagcgtctc gttgaaggcg
     8701 gcgtgaaaat cacctctgtt aacgtcggtg gtatggcatt ccgtcagggt aaaacccagg
     8761 tgaataacgc ggtttcggtt gatgaaaaag atatcgaggc gttcaagaaa ctgaatgcgc
     8821 gcggtattga gctggaagtc cgtaaggttt ccaccgatcc gaaactgaaa atgatggatc
     8881 tgatcagcaa aatcgataag taacgtattg tgttgattat cactcagttt tcacacttaa
     8941 gtcttacgta aacaggagaa gtacaatgga gattaccact cttcaaattg tgctggtatt
     9001 tatcgtagcc tgtatcgcag gtatgggatc aatcctcgat gaatttcagt ttcaccgtcc
     9061 gctaatcgcg tgtaccctgg tgggtatcgt tcttggcgat atgaaaaccg gtattattat
     9121 cggtggtacg ctggaaatga tcgcgctggg ctggatgaac atcggtgctg cagttgcgcc
     9181 tgacgccgct ctggcttcca tcatttctac tattctggtt atcgcaggtc atcagagcat
     9241 tggtgcaggt atcgcactgg caatccctct ggccgctgcg ggccaggtac tgaccatcat
     9301 cgttcgtact attaccgttg ccttccagca cgctgcggat aaggctgctg ataacggcaa
     9361 cctgacagcg atttcctgga tccacgtttc ttctctgttc ctgcaagcaa tgcgtgtggc
     9421 tattccggcc gtcatcgttg cgctgtctgt tggtaccagc gaagtccaga acatgctgaa
     9481 tgcgattccg gaagtggtga ccaatggtct gaatatcgcc ggtggcatga tcgtggtggt
     9541 tggttatgcg atggttatca acatgatgcg tgctggctac ctgatgccgt tcttctacct
     9601 cggcttcgta accgcagcat tcaccaactt taacctggtt gctctgggtg tgattggtac
     9661 tgttatggca gtgctctaca tccaacttag cccgaaatac aaccgcgtag ccggtgcgcc
     9721 tgctcaggca gctggtaaca acgatctcga taacgaactg gactaacagg tgagcgaaat
     9781 ggttgataca actcaaacta ccaccgagaa aaaactcact caaagtgata ttcgtggcgt
     9841 cttcctgcgt tctaacctct tccagggttc atggaacttc gaacgtatgc aggcactggg
     9901 tttctgcttc tctatggtac cggcaattcg tcgcctctac cctgagaaca acgaagctcg
     9961 taaacaagct attcgccgtc acctggagtt ctttaacacc cagccgttcg tggctgcgcc
    10021 gattctcggc gtaaccctgg cgctggaaga acagcgtgct aatggcgcag agatcgatga
    10081 cggtgctatc aacggtatca aagtcggttt gatggggccg ctggctggtg taggcgaccc
    10141 gatcttctgg ggaaccgtac gtccggtatt tgcagcactg ggggccggta tcgcaatgag
    10201 cggcagcctg ttaggtccgc tgctgttctt catcttgttt aacctggtgc gtctggcaac
    10261 ccgttactac ggcgtagcgt atggttactc caaaggtatc gatatcgtta aagatatggg
    10321 tggtggcttc ctgcaaaaac tgacggaagg ggcgtctatc ctcggcctgt ttgtcatggg
    10381 ggcattagtt aacaagtgga cgcatgtcaa catcccgctg gttgtctctc gcattactga
    10441 ccagacgggc aaagaacacg ttaccactgt ccagactatt cttgaccagt taatgccagg
    10501 cctggtacca ctgctgctga cctttgcttg tatgtggctg ttgcgcaaaa aagttaaccc
    10561 gctgtggatc atcgttggct tcttcgtcat cggtatcgct ggttacgctt gcggcctgct
    10621 gggactgtaa gactgttgta cactaccggg gccttttggc cccgtttttt tatctggagg
    10681 attaatgaca atcacggacc tggtactgat tcttttcatc gccgcactcc tggccttcgc
    10741 gatctacgat cagttcatca tgccccgccg taacggcccc accctgctgg caattccttt
    10801 gctccggcgt ggtcgcatcg atagcgttat cttcgtcgga ttgattgtca ttcttatcta
    10861 taacaacgtc acgaatcatg gcgcgctaat aacaacatgg ttattaagtg cactggctct
    10921 gatgggtttt tatatattct ggatccgcgt tccgaagatc atctttaaac aaaaaggttt
    10981 tttcttcgcc aatgtctgga ttgaatatag ccgaatcaaa gcgatgaact tgtcggaaga
    11041 tggcgtgttg gtgatgcaat tagaacagcg ccggctgtta atccgcgttc gaaatatcga
    11101 cgatctggaa aaaatttata agcttct
//
LOCUS       DAZUCC010000170        11027 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZUCC010000170 DAZUCC010000000
VERSION     DAZUCC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.171"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1412
                     /locus_tag="ACHY09_003802"
     CDS             <1..1412
                     /locus_tag="ACHY09_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFS3886712.1"
                     /translation="LILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIVGLVIKPFP
                     EPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAAFVTTGLGK
                     RIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSVAVALGSEP
                     EKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISWGGWALAAG
                     LPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLGVFVLALLG
                     WIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGIIGLSSLLSK
                     VKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAMLPVFAMLAN
                     VSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFL
                     VHITLGVWWWNMLIGWNML"
     gene            1595..3856
                     /locus_tag="ACHY09_003803"
     CDS             1595..3856
                     /locus_tag="ACHY09_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFS3886713.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(3997..5280)
                     /locus_tag="ACHY09_003804"
     CDS             complement(3997..5280)
                     /locus_tag="ACHY09_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFS3886714.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5432..5908)
                     /locus_tag="ACHY09_003805"
     CDS             complement(5432..5908)
                     /locus_tag="ACHY09_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFS3886715.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNIHFHSLASASITAMFS"
     gene            complement(5967..7256)
                     /gene="bioA"
                     /locus_tag="ACHY09_003806"
     CDS             complement(5967..7256)
                     /gene="bioA"
                     /locus_tag="ACHY09_003806"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFS3886716.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHADIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTVAVNRAVQDETFFCQ"
     gene            7343..8383
                     /gene="bioB"
                     /locus_tag="ACHY09_003807"
     CDS             7343..8383
                     /gene="bioB"
                     /locus_tag="ACHY09_003807"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFS3886717.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLASARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            8380..9534
                     /gene="bioF"
                     /locus_tag="ACHY09_003808"
     CDS             8380..9534
                     /gene="bioF"
                     /locus_tag="ACHY09_003808"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFS3886718.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSDTVADYLVQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDTRREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            9521..10276
                     /gene="bioC"
                     /locus_tag="ACHY09_003809"
     CDS             9521..10276
                     /gene="bioC"
                     /locus_tag="ACHY09_003809"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFS3886719.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSMALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            10269..10946
                     /gene="bioD"
                     /locus_tag="ACHY09_003810"
     CDS             10269..10946
                     /gene="bioD"
                     /locus_tag="ACHY09_003810"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFS3886720.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
BASE COUNT         2587 a         2831 c         3024 g         2585 t
ORIGIN      
        1 agctaattct gatattagcg atcccatgta ttattggctt tatgccagct ccggcaggat
       61 taagcgaact ggcgtgggtg ctttttggta tttacctggc ggccattgtg gggctggtta
      121 tcaagccttt cccggaacct gtcgtactgt taattgccgt cgccgcatcg atggtagtgg
      181 tgggtaactt atcaggtggg gaatttaaaa ccaccgccgt attaagcggt tactcttccg
      241 gtaccacctg gctggtgttt tctgcgttta ctttaagcgc cgcgtttgta accacaggct
      301 taggtaaacg tattgcctat ctgctgattg gtaaaattgg tagcactacc ctgggtctgg
      361 gttacgttac ggtatttctc gatctggtac tggctccggc aacaccgtct aacaccgcgc
      421 gtgcgggcgg catcgtgtta ccgatcatca acagcgtggc agtggctttg ggatcagaac
      481 cggaaaaaag tccgcgtcgt gttggacatt acctgatgat gtccatttac atggtcacta
      541 aaaccaccag ttatatgttc tttaccgcaa tggcggggaa cattctggcg ctgaaaatga
      601 tcaacgacat tctgcacctg caaattagct ggggtggatg ggcgctggcc gctggattgc
      661 cgggtatcat tatgctgctg gtcaccccgc tggtgattta caccatgtat ccgccagaaa
      721 ttaagaaggt ggataacaaa accatcgcca aagcgggcct tgccgaactg ggaccgatga
      781 aaatccgcga aaaaatgctg ctcggtgtct tcgtgctggc gctgctgggc tggattttca
      841 gtaagtcact gggggttgat gaatccaccg tggcaatcgt tgttatggcg actatgctgc
      901 tgctgggtat cgttacctgg gaagacgtgg ttaaaaataa aggcggctgg aataccttaa
      961 tctggtacgg cggtattatc ggcttaagct ccttattatc gaaagttaaa ttctttgaat
     1021 ggttagctga agtctttaaa aataacctgg catttgatgg tcacggtaac gttgctttct
     1081 tcgttattat tttcctcagc atcatcgtgc gttatttctt cgcttccggt agtgcctata
     1141 tcgttgctat gttacctgta tttgccatgc tggcgaacgt ctccggcgca ccgttaatgt
     1201 taaccgcgct ggcactgttg ttctctaact cctatggcgg catggttact cactatggcg
     1261 gcgcggcagg tccggtcatc tttggcgtgg gttacaacga tattaaatcc tggtggttgg
     1321 tcggtgcggt actgacgata ttaaccttcc tggtgcatat caccctcggc gtgtggtggt
     1381 ggaatatgct gatcggctgg aacatgctgt aaatatactc gtcatacttc aagttgcatg
     1441 tgctgcgtct gcgttcgctc accccagtca cttacttatg taagctcctg gggattcact
     1501 cgcttgtcgc cttcctgcaa ctcgaattat ttagagtata tccatttatt atctttctgc
     1561 gcacttcacg gtgcgcagat atctggagca tttgatgatc aagttatctg aaaaaggcgt
     1621 gtttctcgcc agcaataacg aaataattgc cgaagaacat ttcaccggcg aaataaaaaa
     1681 agaagaagca aaaaaaggca ctattgcctg gtctattctc tcttctcata atacgtccgg
     1741 aaatatggat aaacttaaaa ttaagtttga ttcattagcc tctcacgata ttacctttgt
     1801 tggtattgta cagaccgcta aagcgtccgg tatggaacgt ttcccgctgc cgtatgtgct
     1861 gaccaactgc cataactcac tctgtgctgt gggcggcacc attaacggtg atgaccatgt
     1921 ttttggttta tcggcggccc agcgttatgg cggtattttt gtgccaccgc atattgcggt
     1981 catccatcaa tatatgcgtg aaatgatggc gggcggcggc aaaatgatcc tcgggtcaga
     2041 cagccatacc cgttacggtg cgttagggac aatggcggtt ggcgagggcg gcggcgagtt
     2101 ggtaaaacag ctgcttaatg acacctggga tatcgactat ccgggcgtgg ttgcggtgca
     2161 tctgaccgga aaacctgcgc cgtatgtggg gccgcaggat gtggcgctgg ctatcatcgg
     2221 tgccgtgttc aaaaacggtt acgtcaaaaa caaagtgatg gagttcgtag gtcccggtgt
     2281 tgcagcgctc tctaccgatt tccgtaacag cgttgacgtg atgaccactg aaacgacctg
     2341 tttaagttct gtctggcaaa ccgatgaaga agtccataac tggctggcgc tgcacggtcg
     2401 cggccaggat tactgccaac ttaaccctca accaatggcg tactacgatg gctgcatcag
     2461 cgttgattta agcgccatca aaccaatgat tgcgctgcca ttccacccga gcaacgtgta
     2521 tgaaatcgac acgctgaacc agaacctgac cgacattctg cgtgagattg aaattgagtc
     2581 cgaacgcgtg gcgcacggta aagccaaact ctcgctgctg gataaagtgg aaaatggtcg
     2641 cctgaaagtg cagcagggga ttatcgcggg ctgttctggc ggtaactacg aaaacgtcat
     2701 cgcggcggcg aatgcgctgc gcggtcaatc ctgcggcaac gataccttct cattggcggt
     2761 ttacccgtca tcacagccgg tgtttatgga tctcgccaaa aaaggtgtgg tagcagattt
     2821 gattggcgca ggcgcaatca tcagaaccgc gttctgcggc ccatgctttg gtgcgggcga
     2881 tacgccaatc aacaacggtt tgagtattcg ccacaccacg cgtaacttcc cgaaccgcga
     2941 aggctctaag ccagctaatg ggcagatgtc agcggtggcg ttgatggacg ctcgttctat
     3001 cgctgcaact gcggcaaacg gtggctattt aacctctgcc agcgaactcg attgctggga
     3061 caacgtgccg gagtacgcct tcgatgtaac gccgtataaa aatcgcgtct atcaaggctt
     3121 tgtgaaaggg gcgacccagc aaccgctgat ctacggaccg aacatcaaag actggccgga
     3181 attgggcgcg ctgactgaca atatcgtcct caaagtgtgc tcgaagatcc tcgacgaagt
     3241 caccaccacc gacgaactga ttccttccgg tgaaacctct tcttatcgtt caaatccgat
     3301 tggtctggcg gagtttaccc tgtcacgccg cgatcccggt tatgttggca gaagtaaagc
     3361 gactgctgag ctggaaaatc agcgtctggc ggggaatgtc agcgagctga cagaggtgtt
     3421 tgcgcgcatt aagcagattg ctggtcagga gcatattgat ccgctgcaaa ctgaaattgg
     3481 cagcatggtc tatgcggtga aaccaggcga tggttctgcg cgtgagcagg cggcgagctg
     3541 ccagcgtgtg attggcggtc tggcgaatat tgccgaggaa tacgcgacta aacgctaccg
     3601 ttctaacgtc atcaactggg ggatgttacc gctgcaaatg gcggaagcac caacgtttga
     3661 agtgggggat tacatttaca tccctggcat taaagcggcg ctggataatc ctggaacgac
     3721 gtttaaaggt tatgtgatcc atgaagatgc accggtaacg gagattacgc tctatatgga
     3781 aagtctgact gctgaagagc gcgagattat caaggcgggt agtttgatta acttcaataa
     3841 aaaccgtcag atgtaaaaag cgccatgtga atgtaggtcg catccggcac ttattgtcgg
     3901 atgcgatgct ggcgcatctt atccgaccta caaatcgcat cgaaccgtag gccggataag
     3961 gcgtttacgc cgcatccggc acatagttaa cagctcctac ttcttcgcct ctgcaaccac
     4021 tttgctaccc acgccgcggt tattgtattc ccacatgcgg ttgtagttag tgtcattcag
     4081 attgcgctgt acttcgtcgt tatcatcaac gctgccggtg ttacccgcga atggacgatt
     4141 ggaaatcacc gcatcagccc acggtttggc tgtgttaaaa ccttcgttga tggcgctatc
     4201 acggatcacc acctgaccgt tggtattggc atcaacatcc agcgagcggc ccagttgcgc
     4261 cacgccatca ccggaagcat tgaaacgact gtttacggcg aggaaaccgt agtaaatgtt
     4321 ggacagcgta gccggtgcaa acacatacgc ttcttgctga gtacgtgagt tcaccacgcg
     4381 gaattcggta ttatcgaaca ccactgcgcc gcgaccagaa acgatatcca catccccttc
     4441 aatgtagctg ttggtcacca gcgtacgcgg ctgacgattc gtttccagac ggttctgcac
     4501 accgctgttg gtgacaaaga aggtgttctg acgaccgaga atgttaacgt tgttaatctg
     4561 tacctggtca ccatcagtac gcagtgccac cgccggatgg ttacccgcat ctacgctatc
     4621 gcccagcgtg ttttcgatgg tcaggttttg cagttgcagt ccattgtttt gtgaccagaa
     4681 gaccgcagag cagagaacgc cgatactgtc gctgcgtttg ctctggcagc tatcgtacat
     4741 ataccacgct ggtttacctg gcatatattt gccgcgcggg ttgacgtcgt gacgccagtc
     4801 ggcggggctc atgcccccat caagggaaag cccaatcttc acatcaatcg gtttttcacc
     4861 tgtaccgtac agagtaattc cacccggagc ggcagggaca tataccgttc cctgatactc
     4921 accaggcatc acggcaatat actggcgctt gttggtacgc ttgataattg ccgcatctac
     4981 cgccgcctga atcgtggtat gcgttacacc ttgagtaccc gccgggccga caacaaagtc
     5041 aggttgtgca ggcagggtaa tcggggaagg attccacgct gccgcacctg gtgtcaggga
     5101 tgcaaaatag tgttgagcat cgaaattctg cgcttctttt gccgacagaa tcgggcgaga
     5161 agaggtacca ggcgcggttt gatcagaagg acgttgatcg ggcggtgttg agctacaggc
     5221 ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg gaaactgaaa atgtgttcac
     5281 aggttgctcc gggctatgaa atagaaaaat gaatccgttg aggcctgctt ttttatacta
     5341 actttagcga aacgggaagg taaaaagaca aaaagttgtt tttaatacct ttaagtgata
     5401 ccagatggct ttgcgccatc tggcagagta attaactgaa catcgcggta atcgatgcgc
     5461 ttgccagaga gtggaaatgg atgttaaacc cgaccatcgc gccgctggca ccttcatcga
     5521 catcaatacg ttctacatcc agcgcatgaa cggtaaaaat gtagcgatgg gtttcgcctt
     5581 tcggcggcgc tgcaccatcg tacccggttt taccaaagtc ggtacgcgtc tgcaaaacgc
     5641 cgtctggcat tgctaccaga ccagagccaa acccttgcgg taatacgcgg gtatcagcgg
     5701 gtaagttaac aactacccag tgccaccagc cggagccggt tggcgcatcc gggtcgtagc
     5761 aggtgacaac aaaacttttc gttcccgcag gaacatcatc ccacgccaga tgcggtgaaa
     5821 tattatcgcc atcgtaaccc atgccgttaa agacatgacg atgtggcaac ttatcgccat
     5881 cgcgcagatc attactgatg agtttcatga accctccttt ctagtttgca gaaagtgtag
     5941 ccagaacccc tcacgctgac tcccggttat tggcaaaaaa atgtttcatc ctgcaccgca
     6001 cggttaaccg ctacggtcag acgctgcaac tgttgcggga gaataatata gggcggcatc
     6061 aggtaaatca gtttgccaaa aggccggatc cagacaccct gttcgacaaa gaatttttgc
     6121 agcgctgcca tattcaccgg acgagtggtt tcgaccacgc cgattgcccc cagtacgcgc
     6181 acatcggcaa ccatttcggc gtcacaggcg ggggcaagtt gctcgcgtag ctgcccttca
     6241 atagccgcca cctgatgctg ccagtcgcca gattcgagaa tcgccaggct ggcgtttgct
     6301 gctgcgcagg ccagcggatt gcccataaaa gttggcccat gcataaagca gccggcttcg
     6361 ccgttactga tggtttccgc aacctcgcgc gtggtgagcg tggcggaaag ggtcattgtg
     6421 ccgccggtta aggctttacc gagacacaaa atatccggcg cgatatctgc atgttcacag
     6481 gcaaacagtt tgccggtacg accaaatccg gtggcgatct cgtcagcaat cagcaagata
     6541 ccttcgcgat cgcacatttt gcggattcgt tttaaccatt ccggatggta gatgcgcatc
     6601 ccgcctgcgc cctggacaat cggttcaatg atcacggcgg cgatttcatg acgatgtgcc
     6661 gccatcaggc gggcaaagcc caccatatcg cgttcatccc attcaccatc catgcggctt
     6721 tgcggggcgg gagcaaacaa gttttctggc agatagcctt tccacaggct gtgcattgag
     6781 ttgtccggat cgcacacaga catcgcgcca aaggtatcgc catgataacc attgcggaag
     6841 gtcagaaaac gctggcgcgc ttcgcctttg gcctgccagt actgcaacgc cattttcatc
     6901 gccacttcca ccgctacgga accggagtcc gcgagaaaaa cgcattccag cggttgcggc
     6961 gtcatcgcca ccagtttgcg gcacagctca atggctggcg catgagtgat accaccaaac
     7021 atcacatgcg acatggcatc aatttgcgtc ttcatcgccg cattaagctg cgggtgattg
     7081 tagccgtgga tcgccgccca ccaggacgac ataccgtcaa ccaggcgtct gccgtcagac
     7141 aaaattagct cgcaaccttc ggcgctcgcc accggataaa ccggtagcgg ggaggtcatg
     7201 gatgtgtatg ggtgccagat atggcgttgg tcaaaggcaa gatcgtccgt tgtcataatc
     7261 gacttgtaaa ccaaattgaa aagatttagg tttacgagtc tacaccgaat taacaacaaa
     7321 aaacacgttt tggagaagcc ccatggctca ccgcccacgc tggacattgt cgcaagtcac
     7381 agaattattt gaaaaaccgt tgctggatct gctgtttgaa gcgcagcagg tgcatcgtca
     7441 gcatttcgat ccacgtcagg tgcaggtcag cactttgctg tcaattaaga ccggagcttg
     7501 tccggaagat tgcaaatact gcccgcaaag ctcgcgctac aaaaccgggc tggaagccga
     7561 gcggttgatg gaagttgaac aggtgctggc gtcggcgcgc aaagcgaaag cggcaggatc
     7621 gacgcgcttc tgcatgggcg cggcgtggaa gaatccccac gaacgcgata tgccctacct
     7681 ggaacaaatg gtgcagggag taaaagcgat ggggctggag gcgtgtatga cgctgggtac
     7741 gttgagtgaa tctcaggcgc agcgccttgc gaatgccggg ctggattact acaaccataa
     7801 cctcgacacc tcgccggagt tttacggcaa tatcatcacc acacgtactt atcaggaacg
     7861 ccttgatacg ctggaaaaag tgcgcgatgc cgggatcaaa gtctgctcag gtggcattgt
     7921 gggtttaggc gaaacggtaa aagatcgtgc cggattatta ctgcaactgg caaacctgcc
     7981 gacgccgccg gaaagcgtgc caatcaacat gctcgtgaag gtgaaaggta ctccactggc
     8041 agataacgat gatgtcgatg cctttgattt tattcgcacc attgccgtag cgcggatcat
     8101 gatgccgacc tcatacgtgc gcctttctgc cggacgcgag cagatgaatg aacagactca
     8161 ggcgatgtgc tttatggccg gggctaactc gattttctac ggttgcaaac tgctaaccac
     8221 gccgaatccg gaagaagata aagacctgca actgttccgc aaactggggc taaatccaca
     8281 gcaaactgcc gtgctggcag gggataacga acaacagcag cgtctggagc aggcactgat
     8341 gaccccggac actgacgaat attacaacgc ggcagcacta tgagctggca ggagaaaatc
     8401 aacgcggcgc tcgatgcgcg gcgtgctgcc gatgccctgc gtcgccgtta tccggtggcg
     8461 caaggggccg gacgctggct ggtggcggac gatcgccagt atctgaactt ttccagtaac
     8521 gattatttag gtttaagcca tcatccgcaa attatccgtg cctggaagca gagtgcggag
     8581 caatttggcg tcggtagcgg cggctccggt cacgtcagcg gttatagcgt ggcacatcag
     8641 gcgctggaag aagaactggc cgagtggctg ggctattcgc gggcgctgct gtttatctcc
     8701 ggttttgcgg ctaatcaggc agtcattgcc gcgatgatgg cgaaagagga ccgtattgtt
     8761 gccgaccggc ttagccatgc ctcattgctg gaggctgcaa gtttaagccc gtcgccgctt
     8821 cgccgttttg ctcataacga tgtcactcat ctggcgcgac tgcttgcttc cccctgtccg
     8881 gggcagcaac tggtagtgac agaaggtgtg ttcagcatgg acggcgatag tgcgccactg
     8941 gaggaaatcc agcaggtaac gcaacagcac gatggctggt tgatggtcga tgacgcccac
     9001 ggcacgggcg ttatcgggga gcaggggcgc ggcagctgct ggctgcaaaa ggtaaaacca
     9061 gaattgctgg tggtgacttt tggcaaagga tttggcgtca gcggggcagc ggtgctttgc
     9121 tccgatacgg tggcggatta tctggtgcaa tttgcccgcc atcttattta cagcaccagt
     9181 atgccgcccg ctcaggcgca ggcattacgt gcatcgctgg cggtcattcg cagtgatgag
     9241 ggtgatacac ggcgcgaaaa actggcggca ctcattacgc gttttcgtgc cggagtacag
     9301 gatttgccgt ttacgcttgc tgattcatgc agcgccatcc agccattgat tgttggtgat
     9361 aacagccgtg cgttacaact ggcagaaaaa ctgcgccagc aaggctgctg ggtcacggcg
     9421 attcgcccgc caaccgtacc tgctggtact gcgcgactgc gcttaacact aacagccgcg
     9481 catgaaatgc aggatatcga ccgtctgctg gaggtgctgc atggcaacgg ttaataaaca
     9541 agccattgca gcggcatttg gacgggcagc cgcacactat gagcaacacg ccgatctaca
     9601 gcgccagagt gctgacgcct tactggcaat gctcccacag cgtaaataca cccacgtact
     9661 ggatgcgggt tgtggaccag gctggatgag ccgccgctgg cgagagcgtc acgcgcaggt
     9721 gactgcgtta gatctctctc cgccaatgct tgttcaggca cgccagaagg atgccgcaga
     9781 ccattatctg gcgggagata tcgaatccct gccgttagcg actgcaacgt tcgatcttgc
     9841 atggagcaat ctcgcggtgc agtggtgcgg taatttatcc atggcactcc gcgagctgta
     9901 tcgggtggtg cgccccggag gcgtggtcgc gtttaccacg ctggtgcagg gatcgttacc
     9961 cgaactgcat caggcgtggc aggcggtgga cgagcgcccg catgctaatc gctttttacc
    10021 gccagataaa atcgaacagt cgctaaacgc cgtgcattat caacattata ttcagcccat
    10081 tacgctgtgg tttgatgatg cgctcagtgc catgcgttcg ctgaaaggca tcggtgccac
    10141 gcatcttcat gaagggcgcg acccgcgaat attaacgcgt tcgcagttgc agcgattgca
    10201 actagcctgg ccgcaacaac aggggcgata tcctctgacg tatcatcttt ttttgggagt
    10261 gattgctcgt gagtaaatgt tattttgtca ccggaacgga taccgaagtg gggaaaactg
    10321 tcgccagctg cgcgctttta caagccgcaa aggcagcagg ctaccggacg gcaggttata
    10381 aaccggtggc ctctggcagc gaaaagaccc cgaaaggctt acgtaatagt gacgcgctgg
    10441 cgttacagcg caacagtagc ctgcagctgg attacgcaac ggtaaatcct tacaccttcg
    10501 cagaacccac ctcgccgcat atcatcagcg cgcaagaggg caggccgata gaatcattgg
    10561 taatgagcgc cggattacgc gcgcttgaac aacaggctga ctgggtgtta gtggaaggtg
    10621 ctggcggctg gtttacgccg ctttctgaca ctttcacttt tgcggattgg gtaacacagg
    10681 aacaactgcc ggtgatactg gtagttggtg tgaaactcgg ctgtattaat cacgcgatgt
    10741 tgactgcgca ggcaatacta cacgccggac tgactctggc ggggtgggtg gcgaacgatg
    10801 tcacgcctcc gggaaaacgt cacgctgaat atatgaccac gctcacccgc atgattcccg
    10861 cgccgctgct gggcgaaatt ccctggcttg cagaaaatcc agaaaatgcg gcaaccggaa
    10921 agtacatcaa tcttgcctta atgtagtcat cctgtaattg gttaaattgc gagcgagatc
    10981 gtgtctccga ttgactgcaa tttaaccaat taaattctaa aaaaatc
//
LOCUS       DAZUCC010000171        10859 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZUCC010000171 DAZUCC010000000
VERSION     DAZUCC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.172"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..290
                     /locus_tag="ACHY09_003811"
     CDS             117..290
                     /locus_tag="ACHY09_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886721.1"
                     /translation="MKKLALILVIGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCIN"
     gene            316..384
                     /gene="yncP"
                     /locus_tag="ACHY09_003812"
     CDS             316..384
                     /gene="yncP"
                     /locus_tag="ACHY09_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFS3886722.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(362..511)
                     /gene="hokB"
                     /locus_tag="ACHY09_003813"
     CDS             complement(362..511)
                     /gene="hokB"
                     /locus_tag="ACHY09_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFS3886723.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            910..2550
                     /gene="trg"
                     /locus_tag="ACHY09_003814"
     CDS             910..2550
                     /gene="trg"
                     /locus_tag="ACHY09_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFS3886724.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2588..3511)
                     /gene="ydcI"
                     /locus_tag="ACHY09_003815"
     CDS             complement(2588..3511)
                     /gene="ydcI"
                     /locus_tag="ACHY09_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3886725.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3728..5071
                     /gene="ydcJ"
                     /locus_tag="ACHY09_003816"
     CDS             3728..5071
                     /gene="ydcJ"
                     /locus_tag="ACHY09_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS3886726.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGETHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5296..6951
                     /gene="opgD"
                     /locus_tag="ACHY09_003817"
                     /gene_synonym="mdoD"
     CDS             5296..6951
                     /gene="opgD"
                     /locus_tag="ACHY09_003817"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFS3886727.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTDFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            7091..7315
                     /gene="ydcH"
                     /locus_tag="ACHY09_003818"
     CDS             7091..7315
                     /gene="ydcH"
                     /locus_tag="ACHY09_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFS3886728.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            7378..7917
                     /gene="rimL"
                     /locus_tag="ACHY09_003819"
     CDS             7378..7917
                     /gene="rimL"
                     /locus_tag="ACHY09_003819"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFS3886729.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNRTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(7909..8889)
                     /gene="ydcK"
                     /locus_tag="ACHY09_003820"
     CDS             complement(7909..8889)
                     /gene="ydcK"
                     /locus_tag="ACHY09_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFS3886730.1"
                     /translation="MRKYRLSEEQRAFSYQDDGTKKSVLLRQIIAMSDFNDVIAGTAG
                     GWIDCETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            9013..10005
                     /gene="tehA"
                     /locus_tag="ACHY09_003821"
     CDS             9013..10005
                     /gene="tehA"
                     /locus_tag="ACHY09_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFS3886731.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLIRTERAVLMKAEDKE"
     gene            10002..10595
                     /gene="tehB"
                     /locus_tag="ACHY09_003822"
     CDS             10002..10595
                     /gene="tehB"
                     /locus_tag="ACHY09_003822"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFS3886732.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLENLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         2766 a         2541 c         2753 g         2799 t
ORIGIN      
        1 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
       61 aaaccagcgt ataacgttaa aatcgaacat aagattcgat aaaccatgga ggttatatga
      121 aaaaactggc acttatcttg gttataggaa cgcttgtttc cttttatgcc gatgccgggc
      181 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
      241 tttgtaatga tggttctatt agtgcatcga aaaaaacatg cattaactga agtgtaaaag
      301 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
      361 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
      421 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
      481 gcagacacac caccagaggg ttgcgcttca tagcctttct ccttgccgga tggcgggtaa
      541 gaggctaaga tctgaattgc tgtgttcatt cgttggcctc ggttgataga aatatcggtc
      601 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgtg
      661 agcatcctat gtggtttcct tagggacgaa aataatcacc tgacgaaatt gcgtgctgtt
      721 ttccagaatt tttcgtcatt cgggttagcc agtttaatca ttcgttactc tcttcattcc
      781 aatggcatta ttcttctctg ccataattgt tgtaaaaatg tgacgcaaag aggtttttgg
      841 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
      901 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
      961 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     1021 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     1081 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     1141 attcaggccg gggcagcgag tcgtattgcg gaaatggaag caatgaagcg aaatattgcg
     1201 caagccgaat cggagattaa acagtcgcag caaggttatc gtgcttatca gaatcggtcg
     1261 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt ccaggcttat
     1321 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     1381 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgagcaaa
     1441 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     1501 cgcctgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     1561 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     1621 gaaaaaatcg ccagtggcga tctgacgatg aatgatgaac cggcgggtcg taatgaaatc
     1681 ggtcgcttaa gtcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     1741 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttccgc tggcaatgcg
     1801 gacctttcat ctcgcaccga agaacaagcg gcggcaatcg aacaaactgc cgccagcatg
     1861 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     1921 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     1981 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     2041 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     2101 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagccgc
     2161 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     2221 ctgggatcgg atgaggtggc cacggccggg aaaaccatga gcactattgt tgatgccgtc
     2281 gcgagtgtca cacatatcat gcaggaaatt gccgccgcct cggatgagca aagtaggggt
     2341 ataacgcagg ttagccaggc gatatctgaa atggataagg tgacgcaaca gaatgcttct
     2401 ctggtagaag aggcctcagc agcggcagtg tcccttgaag aacaggcggc acgattaact
     2461 gaggcggtgg acgtattccg tctgaacaaa cattctgtgt cggcagaacc tcgcggagct
     2521 ggtgaaccag ttagtttcgc tacagtgtga aaatgttaaa ggagggatcg acagatccct
     2581 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     2641 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgacccggaa
     2701 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     2761 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     2821 tacaacccga aggaatttta catccctggc tctgcactaa tgcatctgaa tgctggcgtg
     2881 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     2941 tctcctgaag tagcggatga ttagggcgga caaccagctt cagcgattca agaaacagca
     3001 gttcgtaatt aagtccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     3061 tttccccggt ttttaaaccc gcaagaatca tagggttact cattgtcgca acttgcaagg
     3121 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     3181 cagtcggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     3241 actgtccagc agtgttgatg gcgtcaagca ctctgactgc atgcgttaaa aattgttcgc
     3301 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     3361 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     3421 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     3481 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     3541 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatattatta acaattaaag
     3601 caattatgtt acagcgaaat ggataatatt gatgttttcg cggcgagatc acagtttgca
     3661 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     3721 agacattatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     3781 gtcagccatg taccagcaag aagttccgca gtacggtacg ctgctggaac tggtagctga
     3841 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     3901 cgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgtgttggga ctgcacaaga
     3961 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     4021 tctctcgcag gcgggggtgc cggtacattc cacagcattt cggcccattg atgatgcttc
     4081 tctggcgcgt aatcccttcc gcgtttttac ttcattactc cgccttgagc ttatcgagaa
     4141 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     4201 ttgtcgacaa ctgttagagg aatatgagca gcggggcggt tttaacgaaa cacaggcaca
     4261 ggagtttgtg caggaagccc tggaaacgtt tcgttggcac cagtcagcaa cggtagatga
     4321 agaaacctat cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc
     4381 tggatgccat atcaaccacc tgacgccacg cacgctggat attgaccggg tgcagtcgat
     4441 gatgcctgaa tgcggaattg aacccaaaat tctgatcgaa gggccgccgc gccgcgaggt
     4501 accgatttta ctacgccaga ctagctttaa agcactggaa gagacggtgt tgtttgcagg
     4561 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaata gaacagcgtg gcgtggcatt
     4621 aacgccgaaa ggacgacaac tgtatgatga tctactgcgg aacgctggaa ccgggcagga
     4681 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     4741 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt caggtgagac
     4801 gcatcgtcag gcgattcatc ccggagacga tccacagccc ttaattgaac gtggttgggt
     4861 agtggcgcaa cccattacct atgaagattt cttgcctgtt agcgcggcgg ggatcttcca
     4921 gtcaaatctg ggtaatgaaa cgcaggcacg cagtcacggt aatgccagtc gcgaagcatt
     4981 tgagcaggcg ttgggctgcc cggttttgga tgagttccag ctttatcagg aagcggaaga
     5041 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     5101 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaattg ccgatttatg
     5161 catattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     5221 aaataagcac tttcttgccg ctgaaaacga tcagcgcggg accattcaca acaccagaag
     5281 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     5341 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     5401 gatattgccg acgggcaaac ccaacgtttt gacttctcca ttctacagtc aatggcgcac
     5461 gacttagcgc aaacagcgtg gcgtggtgcg ccgcgtccgt tacctgacac tctggcgaca
     5521 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     5581 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     5641 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     5701 gagttgttca aatacaacga tgcgggtgtt gataccaaac aattagaagg gcaaagtgat
     5761 ctcggttttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtcgta
     5821 tcattcctcg gcgcgagtta tttccgcgcg gttgatgata catatcaata cggtttgtcg
     5881 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttact
     5941 gacttctggt ttgatacggt aaaaccgggg gcaaccacct ttaccgttta tgcgttgctc
     6001 gatagcgcca gcattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     6061 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     6121 ccgatgacca gcatgttcag ctgcggtact aatgaacgtc ggatgtgcga caccattcat
     6181 ccgcaaatcc atgactctga tcgtttgtcc atgtggcggg gcaatggcga gtggatttgt
     6241 cgtccgctga ataatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     6301 gggtttggtt tattgcaact ggatcgtgat ttctcccatt atcaggacat tatgggctgg
     6361 tataacaaac gcccaagtct gtgggtggag ccgcgtaaca agtggggtaa gggcaccatc
     6421 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag
     6481 ccagaaaaag cggtaaaagc gggtgatgag tttgcattcc agtatcgtct gtactggagt
     6541 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     6601 ggtttcccgg aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgacgtttt
     6661 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     6721 attacgcttt ccagtgggga agcgaaacaa atcgaaattc tctatattga acccattgat
     6781 ggttatcgta ttcagtttga ctggtatccg acttcggact ccactgatcc ggtcgatatg
     6841 cggatgtatc tgcgttgtca gggcgacgct atcagtgaaa catggctgta tcagtatttc
     6901 ccgccagcgc cggataaacg tcagtatgtt gatgaccgcg tgatgagtta atcgtttttt
     6961 cttcggcatc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     7021 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     7081 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     7141 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     7201 aggttccgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     7261 gttaaaagat gagatgctca aaatcctgca gcaggtgagc gtcaaagagg tgtaaacttt
     7321 cctaagccgc cagccaggcg gcttttttaa caactgcatg gattgactgg agataagatg
     7381 actgaaacga taaaagtaag tgaatcactt gaattacatg ctgttgcaga aagtcatgtc
     7441 acacctcttt atcagttaat ctgtaaaaat agaacctggt tacagcagtc gctaaactgg
     7501 ccgcagtttg ttcaaagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     7561 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     7621 atctcgttta atcgtattga accactgaat aaaaccgctg aaatcggcta ctggctggac
     7681 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     7741 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tgcgcaaagc
     7801 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gtctgaaaca ggctgagttc
     7861 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc acaataaccc
     7921 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     7981 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catgtttttc
     8041 aattatcacc gcacctgtaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     8101 aatcggtccg ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     8161 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     8221 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcac aatcgcatag
     8281 ccagacgttg ttctcttcat taccttcaac gctggcaaaa tcaaaaactt cggcgcgatg
     8341 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt gaacaatacg
     8401 cgaggcgcta acccttgcgc gatcataaat ttgtagcagg agttgatggt cgggcgttaa
     8461 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
     8521 ttgcccgcaa actaatgagt cacgaatggt gacactgtca cttatatatg caccctgact
     8581 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagtacgct
     8641 ggtgccggtg atgcgcgtat taccggaaat caccgctccg ccgaacgcaa tagcattctg
     8701 gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt tcgcaatcga tccagccgcc
     8761 agcggttcct gctatcacat cgttaaaatc gctcatggcg ataatctgcc gtaataacac
     8821 acttttttta gtgccatcat cctgataact aaaggctcgc tgttcttcac ttagacgata
     8881 tttgcgcata gcgttttccc acaggtgact tactataacc gtagcaaatt ctgcggctct
     8941 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
     9001 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
     9061 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
     9121 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
     9181 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agtgcgccat
     9241 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
     9301 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggcgt cgtggttcag
     9361 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
     9421 accacgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
     9481 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
     9541 ctaagccttg aacctgtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggcg
     9601 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggctg
     9661 agcgtcaacg gcggcgaggg tgacacgctg gcgaaaatgc ttttcggtta tggactgctg
     9721 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
     9781 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
     9841 ggcagcgata atggattttt ccatacgctg gcggtgccgc tgtttatctt taccaatttt
     9901 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttaatc
     9961 agaaccgagc gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
    10021 actattttac tgataaatat gaattaactc gcacacactc tgaagtgctg gaagcggtga
    10081 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
    10141 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
    10201 acgtcgagcg gattaaatcc attgaaaatc tggagaattt acacacccgg gtcgttgatc
    10261 tgaataacct cacatttgat ggacagtatg attttattct ttcgactgtg gtgctgatgt
    10321 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacctg
    10381 gcggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    10441 tcccgtttgc cttcaaagag ggggaattac gtcgatatta cgaaggctgg gagatggtga
    10501 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    10561 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taggaacaag gcgcgtgaaa
    10621 attccccacg ctgagatggt ttactgttct tcttttcggt aagcatattt tttatcgaat
    10681 ggatgtgaaa ttaatcacag tagtcgaaat tttttagcag cttaacttac tgaaatttaa
    10741 ctactgatga ttgacttagc ccctttttcg gcagcccctt tttcggcatt gactatgtct
    10801 ttttcggcat tgactatgtc gtctgaaaag gggctaaaaa atttattttc accaacact
//
LOCUS       DAZUCC010000172        10821 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZUCC010000172 DAZUCC010000000
VERSION     DAZUCC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.173"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            208..1374
                     /gene="nhaA"
                     /locus_tag="ACHY09_003823"
     CDS             208..1374
                     /gene="nhaA"
                     /locus_tag="ACHY09_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFS3886733.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNHADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1434..2339
                     /gene="nhaR"
                     /locus_tag="ACHY09_003824"
     CDS             1434..2339
                     /gene="nhaR"
                     /locus_tag="ACHY09_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFS3886734.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(2435..2698)
                     /gene="rpsT"
                     /locus_tag="ACHY09_003825"
     CDS             complement(2435..2698)
                     /gene="rpsT"
                     /locus_tag="ACHY09_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS3886735.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            2801..3019
                     /locus_tag="ACHY09_003826"
     CDS             2801..3019
                     /locus_tag="ACHY09_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFS3886736.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3027..3968
                     /gene="ribF"
                     /locus_tag="ACHY09_003827"
     CDS             3027..3968
                     /gene="ribF"
                     /locus_tag="ACHY09_003827"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFS3886737.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4011..6827
                     /gene="ileS"
                     /locus_tag="ACHY09_003828"
     CDS             4011..6827
                     /gene="ileS"
                     /locus_tag="ACHY09_003828"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS3886738.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSLSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCANAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            6827..7321
                     /gene="lspA"
                     /locus_tag="ACHY09_003829"
     CDS             6827..7321
                     /gene="lspA"
                     /locus_tag="ACHY09_003829"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS3886739.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            7429..7878
                     /gene="fkpB"
                     /locus_tag="ACHY09_003830"
     CDS             7429..7878
                     /gene="fkpB"
                     /locus_tag="ACHY09_003830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS3886740.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            7880..8830
                     /gene="ispH"
                     /locus_tag="ACHY09_003831"
                     /gene_synonym="lytB"
     CDS             7880..8830
                     /gene="ispH"
                     /locus_tag="ACHY09_003831"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFS3886741.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKGVNCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            8896..9810
                     /gene="rihC"
                     /locus_tag="ACHY09_003832"
     CDS             8896..9810
                     /gene="rihC"
                     /locus_tag="ACHY09_003832"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFS3886742.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            9977..10798
                     /gene="dapB"
                     /locus_tag="ACHY09_003833"
     CDS             9977..10798
                     /gene="dapB"
                     /locus_tag="ACHY09_003833"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS3886743.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         2463 a         2623 c         3028 g         2707 t
ORIGIN      
        1 aatcctgacc ttaagctatg attatcaggg cttagggtca cacgtcagcg cttacagccg
       61 tcaaaaactc atctcatcac tgatgacgca aatccttcaa tgcctcgtaa aaagcgaatt
      121 attcctacac tataatctga ttttaacgat gattcgtgcg gggtaagata gtaaaaacga
      181 tctattcacc tgaaagagaa ataaaaagtg aaacatctgc atcgattctt tagcagtgat
      241 gcctcgggag gcattattct tatcattgcc gctatcctgg cgatgattat ggccaacagc
      301 ggcgcaacca gtggatggta tcacgacttt ctggagacgc cggttcagct ccgggttggt
      361 tcactcgaaa tcaacaaaaa catgctgtta tggataaatg acgcgctgat ggcggtattt
      421 ttcctgttag tcggtctgga agttaaacgt gaactgatgc aaggatcgct agccagctta
      481 cgccaggctg catttccagt tatcgccgct attggtggga tgattgtgcc ggcattactc
      541 tatctggcct ttaaccatgc cgatccgatt acccgcgaag ggtgggcgat cccggcggct
      601 actgacattg cttttgcact tggtgtgctg gcgctgttgg gaagtcgtgt tccgttagcc
      661 ctgaagatct ttttgatggc tctggctatt atcgacgatc ttggggccat cattatcatc
      721 gcattgttct acactaatga cttatcgatg gcctctcttg gcgtcgcggc tgtagcaatt
      781 gcggtactcg cggtattgaa tctgtgtggt gtacgccgca cgggcgtcta tattctggtt
      841 ggcgtggtgt tgtggactgc ggtgttgaaa tcgggggttc acgcaaccct ggcgggggta
      901 attgtcggct tctttattcc tttgaaagag aagcatgggc gttctccagc gaagcgactg
      961 gagcatgtgt tgcacccgtg ggtggcgtat ctgattttac cgctgtttgc atttgctaat
     1021 gctggcgttt cactgcaagg cgtcacgctg gatggcttga cctccattct gccattgggg
     1081 atcatcgctg gcttgctgat tggcaaaccg ctggggatta gtctgttctg ctggttggcg
     1141 ctgcgtttga aactggcgca tctgcctgag ggaacgactt atcagcaaat tatggcggtg
     1201 gggatcctgt gcggtatcgg ttttactatg tctatcttta ttgccagcct ggcctttggt
     1261 agcgtagatc cagaactgat taactgggcg aaactcggta tcctggtcgg ttctatctct
     1321 tcggcggtaa ttggatacag ctggttacgc gttcgtttgc gtccatcagt ttgacaggac
     1381 ggtttaccgg ggagccataa acggctccct tttcattgtt atcagggaga gaaatgagca
     1441 tgtctcatat caattacaac cacttgtatt acttctggca tgtctataaa gaaggttccg
     1501 tggttggcgc agcggaggcg ctttatttaa caccacaaac cattaccgga cagattcggg
     1561 cgctggaaga gcgcctgcaa ggcaaattat ttaagcgcaa gggacgtggc ctcgagccca
     1621 gcgagctggg agagctggtc tatcgctatg ccgataaaat gttcacctta agccaggaaa
     1681 tgctggatat cgtcaactat cgcaaagagt ccaacttatt gtttgatgtt ggtgtggcag
     1741 atgcactttc caaacgtctg gtcagcagtg ttctggatgc cgcagttgtg gaagacgagc
     1801 agatccatct acgctgtttc gaatcgacgc acgagatgct tttagagcag ttgagtcagc
     1861 ataaactgga tatgatcatc tctgactgtc cgatcgattc cactcagcag gaagggctgt
     1921 tttccatgaa aattggcgaa tgtggtgtca gtttctggtg cactaaccca ctaccagaaa
     1981 agccgtttcc tgcctgtctt gaagagcgtc gtttacttat tccggggcgt cgctcaatgt
     2041 tggggcgtaa gctattaaac tggtttaact cccagggctt gaacgtcgaa attttgggtg
     2101 agtttgatga tgctgcgttg atgaaagcct ttggggcgac gcataacgct attttcgttg
     2161 caccttcgct ttacgctaat gatttctata acgatgactc ggttgtggag ataggccgtg
     2221 ttgagaacgt gatggaagag taccacgcga tttttgccga aaggatgatt cagcaccctg
     2281 cagtacagcg tatctgcaat acagactatt ctgcgctgtt tactccagct tcaaaataaa
     2341 catttgtact ttgcaggccg aattttggcc tgcagatgga tataaaaaaa cccgccagag
     2401 cgggtttttt tacaaagctg acaacaacag gcaattaagc cagtttgttg atctgtgcag
     2461 tcaggttagc cttatgacgt gcagctttgt ttttgtggat cagaccttta gcagcctgac
     2521 ggtccacgat tggttgcatt tcgttaaatg ctttctgtgc agcagctttg tcgccagctt
     2581 cgatagctgc gtatactttc ttgatgaaag tacgcatcat agagcgacgg cttgcgttgt
     2641 gcttacgagc cttttcagac tgaatggcgc gcttcttagc tgatttgata ttagccaagg
     2701 tccaactccc aaatgtgttc tatatggaca attcaaaggc cgaggaatat gcccttttag
     2761 ccttcttttg tcaatggatt tgtgcaaata agcgccgtta atgtgccggc actcgttacg
     2821 tagtgatggc gcaggattct accagcttgc ggggtgtgaa tacagctttt ccgcgagaaa
     2881 aattgcagca ggcggtcagt ttcttcccat gatttgcgcc atagcaatga aaagccactt
     2941 ctttctgatt tcggtactca atcgccggtt aaccttgacc gctgtacaag gtatactcgg
     3001 acgattttca ctgttttgag ccagacatga agctgatacg cggcatacat aatctcagcc
     3061 aggccccgca agaagggtgt gtgctgacta ttggtaattt cgacggcgtg catcgcggtc
     3121 atcgcgcgct gttacagggc ttgcaggaag aagggcgcaa gcgcaactta ccggtgatgg
     3181 tgatgctttt tgaacctcaa ccactggaac tgtttgctac tgataaagcc ccggcacggc
     3241 tcacccggct gcgggaaaaa ctgcgttatc ttgcagagtg tggtgttgat tacgtgctgt
     3301 gcgtgcgttt tgacaggcgt tttgcggcgt taaccgcgca aaacttcatc agtgatcttc
     3361 tggtgaagca cttgcgggta aaatttcttg ccgtaggtga cgatttccgc tttggcgctg
     3421 gtcgtgaagg cgatttcttg ttattacaga aagcgggcat ggaatacggc ttcgatatca
     3481 ccagcacgca aactttttgc gaaggtggcg tgcgtatcag cagcaccgcc gtgcgtcagg
     3541 cgcttgcgga tgacaatctg gctctggcag aaagtttact ggggcacccg tttgctatct
     3601 ccgggcgtgt agtccacggt gatgaattag ggcgcactat aggtttcccg acggcgaatg
     3661 taccgctacg ccgtcaggtt tccccggtga aaggggttta tgcggtagaa gtgttgggcc
     3721 ttggcgaaaa gccgttaccc ggcgttgcaa acatcggaac acgcccaacg gttgccggta
     3781 ttcgccagca actggaagtg catttgttag atgttgcaat ggacctttat ggtcgccata
     3841 tacaagtagt gctgcgtaaa aaaatacgca atgagcagcg atttgcatcg ctggacgaac
     3901 tgaaagcgca gattgcgcgt gatgaattaa ccgcccgcga attttttggg ctaacaaaac
     3961 cggcttaagc ctgttatgta atcaaaccga aatacggaac cgagaatctg atgagtgact
     4021 ataaatcaac cctgaatttg ccggaaacag ggttcccgat gcgtggcgat ctcgccaagc
     4081 gcgaaccggg aatgctggcg cgttggactg atgatgatct gtacggcatc attcgtgcag
     4141 ctaaaaaagg caaaaaaacc ttcattctgc atgatggccc tccttatgcg aatggcagta
     4201 ttcatattgg tcactcggtt aacaagattc tgaaagacat tatcattaag tccaaagggc
     4261 tttctggata tgactcgccg tatgtgcctg gctgggactg tcatggtctg ccaatcgagc
     4321 tgaaagtaga gcaagaatac ggtaagccgg gggagaaatt caccgccgct gagttccgcg
     4381 ccaagtgccg cgaatacgct gcgacccagg ttgacggtca gcgcaaagac tttatccgtc
     4441 tgggcgtgct gggcgactgg tcgcacccgt acctgaccat ggacttcaaa actgaagcca
     4501 acatcatccg cgcgctgggc aaaatcatcg gcaacggtca cctgcacaaa ggcgcgaagc
     4561 cggtgcactg gtgcgttgac tgccgttctg cactggcaga agcggaagtt gagtattacg
     4621 acaaaacttc tctgtccatc gacgtcgctt tccaggcggt cgatcaggat gcgcttaaaa
     4681 cgaaatttgg cgtaagcaat gttaacggcc caatttcgct ggttatatgg accaccacgc
     4741 cgtggacgct gcctgctaac cgcgcaatct ccatcgcacc tgattttgat tatgcgctgg
     4801 tgcaaatcga cggtcaggcc gtgatcctcg cgaaagatct ggttgaaagc gtaatgcagc
     4861 gtatcggcgt tagcgattac accattcttg gcacggtgaa aggtgccgag ctggaactgt
     4921 tgcgctttac ccatccgttt atggacttcg atgttccggc aattctcggc gaccacgtta
     4981 cgctggatgc cggtaccggt gccgttcata ccgcgccagg ccacggtccg gacgactatg
     5041 tgatcggtca aaaatatggt ctggaaaccg ctaacccggt tggcccggac ggcacttatc
     5101 tgccgggtac ttacccgact ctggatggcg ttaacgtctt caaagcgaac gatattgtca
     5161 ttgcgttgtt gcaggaaaaa ggcgcactgt tgcacgttga gaaaatgcaa cacagctatc
     5221 cgtgctgctg gcgtcataaa acgccgatca tcttccgcgc gacgccgcag tggttcgtca
     5281 gcatggatca gaaaggtctg cgtgcgcagt cactgaaaga gatcaaaggc gtgcagtgga
     5341 tccctgactg gggccaggcg cgtatcgagt cgatggttgc taaccgtcct gactggtgta
     5401 tctctcgtca gcgtacctgg ggcgtgccga tgtcactgtt cgtgcacaaa gacacagaag
     5461 aactgcatcc gcgtactctc gaactgatgg aagaagtggc aaaacgcgtt gaagttgacg
     5521 gcattcaggc gtggtgggat ctcgatgcga aagagatcct cggcgacgaa gctgaccagt
     5581 atgtgaaagt accggatacg ctggatgtat ggtttgactc cggatctacc cactcttccg
     5641 ttgttgatgt gcgtccggaa tttgccggtc acgcagcgga catgtatctg gaaggttctg
     5701 accaacaccg tggctggttc atgtcttccc tgatgatctc taccgcgatg aagggcaaag
     5761 cgccatatcg tcaggtactg actcacggct ttaccgtgga tggtcagggt cgcaagatgt
     5821 ctaaatccat cggtaacacc gtttcgccgc aggatgtgat gaacaaactg ggtgcggata
     5881 ttctgcgtct gtgggtggca tcaaccgact acactggcga aatggccgtt tctgacgaga
     5941 tcctgaaacg tgctgccgac agctatcgtc gtatccgtaa caccgcgcgc ttcctgctgg
     6001 caaacctgaa cggttttgat ccggcaaaag atatggtgaa accggaagag atggtggtac
     6061 tggatcgctg ggccgtaggt tgtgcgaacg cggcacagga agacatcctc aaagcgtacg
     6121 aagcatacga tttccacgaa gtggtacagc gtctgatgcg cttctgctcc gttgagatgg
     6181 ggtccttcta cctcgacatc atcaaagacc gtcagtatac cgccaaagcg gacagcgtgg
     6241 cgcgtcgtag ctgccagact gcgctgtatc acatcgcaga agcgctggtt cgctggatgg
     6301 caccaatcct ctccttcacc gctgatgaag tgtggggtta cctgccgggc gaacgtgaaa
     6361 aatacgtctt caccggtgag tggtacgaag gcctgtttgg tctggcagac agtgaagcaa
     6421 tgaacgatgc gttctgggac gagctgttga aagtgcgtgg cgaagtgaac aaagtcattg
     6481 agcaagcgcg tgccgataag aaagtgggcg gctcgctgga agcggcagta accttgtatg
     6541 cagaaccgga actggcggcg aaactgaccg cgctgggcga tgaattacga tttgtcctgt
     6601 tgacctccgg cgctaccgtt gcagactata acgacgcacc tgctgacgcc cagcagagcg
     6661 aagtcctcaa agggctgaaa gtcgcgttga gtaaagccga aggtgagaag tgcccacgct
     6721 gctggcacta cacccaggat gtcggcaagg tggcggaaca cgcagaaatc tgcggccgct
     6781 gtgtcagcaa cgtcgccggt gacggtgaaa aacgtaagtt tgcctgatga gtcaatcgat
     6841 ctgttcaaca gggctacgct ggctgtggct ggtggtagtc gtgctgatta tcgatctggg
     6901 cagcaaatac ctgatcctcc agaactttgc tctgggggat acggtcccgc tgttcccgtc
     6961 gcttaatctg cattatgcgc gtaactatgg cgcggcgttt agtttccttg ccgatagtgg
     7021 cggctggcag cgttggtttt ttgccggtat tgcgattggt attagcgtga tcctggcagt
     7081 gatgatgtat cgctcgaagg ccacgcagaa gctaaacaat atcgcttacg cgctgattat
     7141 tggcggcgcg ctgggcaacc tgttcgaccg cctgtggcac ggcttcgttg tcgatatgat
     7201 cgacttctat gtcggcgact ggcacttcgc caccttcaac cttgccgata ctgccatttg
     7261 tgtcggtgcg gcactgattg tgctggaagg ttttttacct tctaaagcga aaaaacaata
     7321 ataaaccctg ccggatgcga tgctgacgca tcttatccgg cctacagatt gctgcgaaat
     7381 cgtaggccgc atccggcaaa aatcctcaaa atataagagc aaacctgcat gtctgaatct
     7441 gtacagagca atagcgccgt cctggtgcac ttcacgctga aactcgatga tggcaccacc
     7501 gctgagtcta cccgcaacaa cggtaaaccg gcgctgttcc gcctgggtga tgcttctctt
     7561 tctgaagggc tggagcaaca cctgctgggg ctgaaagtgg gcgataaaac caccttctcg
     7621 ctggagcctg atgcggcgtt tggcgtgccg tcaccggacc tgattcagta cttctcccgc
     7681 cgtgaattta tggatgcagg cgagccagaa attggcgcaa tcatgctttt taccgcaatg
     7741 gatggcagtg agatgcctgg cgtgatccgc gaaattaacg gcgactccat taccgttgat
     7801 ttcaaccatc cgctggccgg gcagaccgtt cattttgata ttgaagtgct ggaaatcgat
     7861 ccggcactgg aggcgtaaca tgcagatcct gttggccaac ccgcgtggtt tttgtgccgg
     7921 ggtagaccgc gctatcagca ttgttgaaaa cgcgctggcc atttacggcg caccgatata
     7981 tgtccgtcac gaagtggtgc ataaccgcta cgtggtcgat agcttgcgcg agcgtggggc
     8041 tatctttatt gagcagatta gcgaagtacc ggacggcgcg atcctgattt tctccgcaca
     8101 cggtgtttct caggcagtac gtaacgaagc gaaaagccgc gatttgacgg tgtttgatgc
     8161 cacctgtccg ctggtgacca aagtgcatat ggaagtcgcc cgcgccagcc gtcgtggtga
     8221 agaatctatt ctcatcggtc acgccgggca cccggaagtg gaagggacga tgggccagta
     8281 cagcaacccg gaagggggaa tgtatctggt cgaatcgcca gacgatgtgt ggaaactgac
     8341 ggtcaaaaac gaagagaagc tctcctttat gacccaaacc acgctgtcgg tggatgacac
     8401 gtctgatgtg atcgacgcgc tgcgtaaacg cttcccgaaa attgtcggtc cgcgcaaaga
     8461 tgacatctgc tacgccacga ctaaccgtca ggaggcggta cgcgccctgg cagagcaggc
     8521 ggaagtagta ctggtggttg gttcgaaaaa ctcctccaac tccaaccgtc tggcggaact
     8581 ggcccagcgt atgggcaaac gcgcattttt gattgacgat gcgaaagata tccaggaaga
     8641 gtgggtaaaa ggcgtgaact gcgttggcgt gactgcgggc gcatcggctc cggatattct
     8701 ggtgcagaat gtggtggcac gtttgcaaca gctgggcggt ggtgaagcca ttccgctgga
     8761 aggtcgtgaa gaaaacattg ttttcgaagt gccgaaagag ctgcgtgtcg atattcgtga
     8821 agtcgattaa gtcattagca gcctaagtta tgcgaaaatg ccggtcttgt taccggcatt
     8881 ttttatggag aaaacatgcg tttacctatc ttcctcgata cagaccccgg cattgacgat
     8941 gccgttgcca ttgccgccgc gatttttacc cccgaactcg atctgcaact gatgaccact
     9001 gtcgcgggta atgtttcggt agagaaaacg acccgcaacg ccctgcaact gctgcatttc
     9061 tggaatgcgg agattccgct tgcccagggc gctgccgtac cactggttcg tgcgccgcgc
     9121 gatgcggcat ctgtacacgg tgaatcggga atggctggct atgactttgt tgaacacaac
     9181 cgtcagccac tgggtatacc ggcgtttctg gcaattcgtg atgccctgat gcgtgcgcca
     9241 gagcccgtta ccctggtggc catcggcccg ttaaccaata ttgcgctgtt actttcacaa
     9301 tgcccggaat gtaagccgta tattcgccgt ctggtgatca tgggcggttc tgctggtcgc
     9361 ggcaactgca caccaaacgc cgagtttaat atcgctgccg atccagaagc ggcagcctgt
     9421 gtcttccgta gcggcatcga aatcgtcatg tgcggtctgg atgtcaccaa tcaggcaata
     9481 ttaacccctg actatctcgc taccttgccg gagttgaatc gcaccggaaa aatgctccac
     9541 gccctgttta gtcactaccg tagcggcagt atgcaaagtg gactgcgaat gcacgatctc
     9601 tgcgccatcg cctggctggt gcgcccggac ctgttcactc tcaagccgtg ttttgtggca
     9661 gtggaaacgc agggcgaatt tacctctggc acgacggtgg ttgatatcga cggttgcctg
     9721 ggcaaaccag ccaatgtaca ggtggcattg gatctgaatg taaaaggctt ccagcagtgg
     9781 gtagctgagg tgctggctct ggtgccgtaa cctgtcacat gttatcggca tgcagtcatt
     9841 catcgactca tgcctttcac tgatatccct ccctgtttat cattaatttc taattatcag
     9901 cgtttttggc tggcggcgta gcgatgcgct ggttactctg aaaacggtct atgcaaatta
     9961 acaaaagaga atagctatgc atgatgcaaa catccgcgtt gccatcgcgg gagccggggg
    10021 ccgtatgggc cgccagttga ttcaggcggc gctggcatta gaaggcgtgc agctgggcgc
    10081 ggcgctggag cgcgaaggat cttctttact gggcagcgat gcgggcgaac tggccggagc
    10141 cgggaaaaca ggcgttactg tgcaaagtag tcttgatgcg ataaaagatg attttgatgt
    10201 gtttatcgat tttacccgtc cggaaggtac gctgaaccat ctcgcttttt gtcgccagca
    10261 tggcaaaggg atggtgatcg gcactacggg gtttgacgaa gccggtaaac aggcgattcg
    10321 tgacgccgcc gccgatattg cgattgtctt tgccgccaat tttagcgttg gcgttaacgt
    10381 catgcttaaa ctgctggaga aagcagccaa agtgatgggt gactacaccg atatcgaaat
    10441 tattgaagca catcatagac ataaagttga tgcgccgtca ggcaccgcac tggcaatggg
    10501 agaggcgatc gcccacgctc tggataaaga tctgaaagat tgcgcggtct acagtcgtga
    10561 aggccatacc ggtgaacgtg tgcctggcac cattggtttt gccaccgtgc gtgcaggtga
    10621 catcgttggt gaacataccg cgatgtttgc cgatattggc gagcgtctgg agatcaccca
    10681 taaggcgtcc agccgcatga catttgctaa cggtgcggta agatcggctt tgtggttgag
    10741 tggtaaggaa agtggtcttt ttgatatgcg agatgtgctt gatctcaata atctgtaacc
    10801 acaaaatatt tatagtgatg c
//
LOCUS       DAZUCC010000173        10729 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZUCC010000173 DAZUCC010000000
VERSION     DAZUCC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.174"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..1214
                     /gene="yghO"
                     /locus_tag="ACHY09_003834"
     CDS             72..1214
                     /gene="yghO"
                     /locus_tag="ACHY09_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFS3886744.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHYGMIDAIDD
                     PQVFAALFGAAEAWLKSQGANKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1221..1985)
                     /gene="glcC"
                     /locus_tag="ACHY09_003835"
     CDS             complement(1221..1985)
                     /gene="glcC"
                     /locus_tag="ACHY09_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFS3886745.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2236..3735
                     /gene="glcD"
                     /locus_tag="ACHY09_003836"
     CDS             2236..3735
                     /gene="glcD"
                     /locus_tag="ACHY09_003836"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFS3886746.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3735..4787
                     /gene="glcE"
                     /locus_tag="ACHY09_003837"
     CDS             3735..4787
                     /gene="glcE"
                     /locus_tag="ACHY09_003837"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFS3886747.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4798..6021
                     /gene="glcF"
                     /locus_tag="ACHY09_003838"
     CDS             4798..6021
                     /gene="glcF"
                     /locus_tag="ACHY09_003838"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFS3886748.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVTPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGVEAILQTASGCGAFVKEYGQMLKNDALYADKARLVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6026..6430
                     /locus_tag="ACHY09_003839"
     CDS             6026..6430
                     /locus_tag="ACHY09_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFS3886749.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6452..8623
                     /gene="glcB"
                     /locus_tag="ACHY09_003840"
     CDS             6452..8623
                     /gene="glcB"
                     /locus_tag="ACHY09_003840"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFS3886750.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDTAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPSCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPHEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMVE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            8979..10661
                     /gene="glcA"
                     /locus_tag="ACHY09_003841"
     CDS             8979..10661
                     /gene="glcA"
                     /locus_tag="ACHY09_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFS3886751.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2484 a         2697 c         3037 g         2511 t
ORIGIN      
        1 gattattaag atgccgttcg ttgctgttgc aacctgtttt tcaccgttaa ttatttgtta
       61 tccacagaga tatggaatgc gatctgctga tgattaaaat tgaaaaagta attaataaaa
      121 atgaccttaa ggcatttatc gcttttccgt catcacttta tcccgatgat ccaaactgga
      181 tacccccttt attcattgaa cgcagcgagc atttgtctgc gaagaatccg gggacggacc
      241 atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg cgtattaccg
      301 cgcaaatcga taccttgcac cgtgagcgtt acggtgaaga taccggtcat tacggaatga
      361 ttgatgccat tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg gaagcgtggt
      421 taaagtcaca aggtgcaaat aagatcagcg ggcctttcag cctgaatatc aatcaggaaa
      481 gcggattact gattgaaggt tttgacactc cgccctgtgc gatgatgcca cacggcaaac
      541 cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa aggcattgat ttactggcgt
      601 ggtggatgca gcgaaccgat ctcacgttct ctccggcgct aaaaaaactg atggatcagg
      661 tgcgcaaaaa agtgaccatt cgctgcatca atcgccagcg gtttgccgaa gagatgcaga
      721 tcctgcgtga gattttcaat tctggctggc agcacaactg gggatttgtg ccgtttaccg
      781 aacatgaatt cgcgaccatg ggcgatcaac ttaaatatct ggtgccggac gatatgatct
      841 acatcgctga gattgattct gcaccctgcg cgtttattgt tggcttgccg aacatcaacg
      901 aggcgattgc cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc
      961 gtttgaaagt cagcggtgtg cgaactgcgc gagtgccgct gatgggcgtg cgggacgagt
     1021 atcagttcag ccgcatcggc ccggtaattg cgctgttatt gattgaagcc ttacgcgatc
     1081 cgtttgcccg tcggaagatc gatgcgctgg agatgtcatg gatcctcgaa accaatacag
     1141 gcatgaataa catgctggaa cgcattgggg cggagccgta caagcgttac cgattgtatg
     1201 aaaagcagat ttgatggcag tcacccctgc ttcatctcca gcggcaccgc cgagcgaatc
     1261 aaatggtggc cttccagctc gatatcgtgg agattctttt tcacggtccg cacatgatca
     1321 cgtgctgcgc gctgggcgac gtgcggcagc cgctgcaaca ccgcgttgta gatccgcgca
     1381 tgctggcgat cgatctgctt tttttgctgt ggtcgatggt agagattatt tacgctggca
     1441 aacactgaat taaacatcag atcggtcaat gattgcagcg taaacaccag cacctgattg
     1501 tgagaagcct gacaaatggc gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc
     1561 gaaatctctt tgttgttctc actggcggcg agcatttttt cataacagcg ggttatcaca
     1621 acaaaatcag cctgggttcc cagcgttgcc gccagccttg ccgattcgcc ctccagtaat
     1681 gcgcgaacgt cgagcagatc gtacagcgtt cgcggctgcg tactgaacag atggatcagc
     1741 gggctggcgt cctgcacccg attaagccgt gcgacacgag aatcgcgacc ctgcgccgtt
     1801 tcaataatcc cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga gcgtgagaag
     1861 ccgagctttt cacacagtcg acgttccgag ggaagcggct gaccgacctt caacacgccg
     1921 tcgataatta accgttcgat actctctgca accacttcgc aaatagggcg acgttcatct
     1981 ttcattcccg gacctcgtgc acaggtagga ccaatttttc tgttcttaac tcgcaaaaca
     2041 cgcacatcac gcaagtgatt atgttacatc aatttaacat taagttaacc aaggcaaggt
     2101 cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag agatagacga aaacgaaaag
     2161 cccgcttaat aactgttcac agaagcagcg cgcaaaaatc agctgccaca caacacaaca
     2221 aagcgaagcc tattcatgag catcttgtac gaagagcgtc ttgatggcgc tttacccgat
     2281 gtcgaccgca catcggtact gatggcactg cgtgagcatg tccctggact tgagatcctg
     2341 cataccgatg aggagatcat tccttacgag tgtgacgggt tgagcgcgta tcgcacgcgt
     2401 ccattactgg ttgttttgcc aaagcagatg gaacaggtga cggcgattct ggctgtctgc
     2461 caccgcctgc gtgtaccggt ggtgacccgt ggtgcaggca ccgggctttc tggtggcgcg
     2521 ctgccactgg aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat cctcgacatt
     2581 aaccccgttg gtcgccgcgc gcgcgtgcag ccgggcgtgc gtaatctggc gatctcccag
     2641 gccgttgcgc cgcataatct ctattacgcg ccggatcctt cctcacaaat cgcctgttca
     2701 attggcggca atgtggcgga aaatgccggt ggcgttcact gcctgaaata tggtctgacc
     2761 gtacataacc tgctgaaaat tgaagtgcaa acgctggacg gcgaggcgct gacgctggga
     2821 tcggatgcgc tggattcgcc tggttttgac ctgctggcgc tgttcaccgg atcggaaggt
     2881 atgctcggcg tgaccaccga agtgacggta aaactgctgc cgaagccgcc cgtggcgcgg
     2941 gttctgttag ccagctttga ctcggtagaa aaagccggac ttgcggttgg tgacatcatc
     3001 gccaatggca ttatccccgg cgggctggag atgatggata acctgtcgat ccgcgcagcg
     3061 gaagatttta ttcatgccgg ttatcccgtc gacgccgaag cgattttgtt atgcgagctg
     3121 gacggcgtgg agtctgacgt acaggaagac tgcgagcggg ttaacgacat cttgttgaaa
     3181 gcgggcgcga ctgacgtccg tctggcacag gacgaagcag agcgcgtacg tttctgggcc
     3241 ggtcgcaaaa atgcgttccc ggcggtagga cgtatctccc cggattacta ctgcatggat
     3301 ggcaccatcc cgcgccgcgc cctgcctggc gtactggaag gcattgcccg tttatcgcag
     3361 caatatgatt tacgcgttgc caacgtcttt catgccggag acggcaacat gcacccgtta
     3421 atccttttcg atgccaacga acccggtgaa tttgcccgcg cggaagagct gggcgggaag
     3481 atcctcgaac tctgcgttga agttggcggc agcatcagtg gggaacatgg catcgggcgc
     3541 gaaaaaatca atcaaatgtg cgcccagttc aacagcgatg aaatcacgac cttccatgcg
     3601 gtcaaggcgg cgtttgatcc cgatggtttg ctgaaccctg ggaaaaacat tcccacgcta
     3661 caccgctgtg ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc tttccctgaa
     3721 ctggagcgtt tctgatgcta cgcgagtgtg attacagtca ggcgctgctg gagcaggtga
     3781 atcaggcgat tagcgataaa acgccgctgg tgattcaggg aagcaatagc aaagcctttt
     3841 taggccgccc tgtcaccggg caaacgctgg atgtgcgttg tcatcgaggc attgttaatt
     3901 acgacccgac cgagctggtg ataaccgcgc gtgccggaac gccgctggta gccattgaag
     3961 cggcgctgga gagcgccggg caaatgctcc cctgcgagcc gccgcattat ggtgaagaag
     4021 ccacctgggg cgggatggtc gcctgcgggc tggcggggcc gcgtcgcccg tggagcggtt
     4081 cggtccgcga ttttgtcctc ggcacgcgca tcattaccgg cactggaaaa catctgcgtt
     4141 ttggtggcga agtgatgaaa aacgttgccg gatacgatct ctcacggtta atggccggaa
     4201 gctacggttg tctgggtgtg ctcactgaaa tctcgatgaa agtgttaccg cgaccacgcg
     4261 ccaccctgag tctgcgtcgg gaaatcagcc tgcaagaagc catgaatgaa atcgcccagt
     4321 ggcaacttca gccattaccc attagtggct tatgttactt cgacaatgcg ttgtggatcc
     4381 gccttgaggg cggcgaagga tcggtaaaag cagcgcgtga actgctgggg ggcgaagagg
     4441 ttgcgggtca gttctggcag caattgcgtg aacaacaact gccgttcttc tcgttaccag
     4501 gtaccttatg gcgcatttca ttacccagcg atgcgccgat gatggattta cccggcgagc
     4561 aactgatcga ctggggcggg gcgttacgct ggctgaaatc gacagccgat gacaatcaaa
     4621 tccatcgcat cgcccgcaac gctggcggtc atgcgacccg ctttagtgcc ggagatggtg
     4681 gctttgcccc gctaccggct cctttattcc gctatcacca gcagcttaaa cagcagctcg
     4741 acccttgcgg cgtgtttaac cccggtcgca tgtacgcgga actttgagga gcaggctatg
     4801 caaacccgat taacagaaga gatgcggcag aacgcgcgcg cgctggaagc cgacagcatc
     4861 ctgcgcgcct gtgttcactg cggattttgt accgcaacct gcccaaccta tcagcttctg
     4921 ggcgatgaac tggacgggcc gcgcgggcgc atctatctga ttaaacaggt actggaaggc
     4981 aacgaagtca cgcttaaaac acaggagcat ctcgatcgct gtctcacttg ccgcaattgt
     5041 gaaaccacct gtccttctgg tgtgcgctat cacaatttgc tggatatcgg gcgtgatatt
     5101 gtcgagcaga aagtgaaacg cccactgccg gagcgaatgc tgcgcgaagg attgcgccag
     5161 gtagtgccgc gtccggcggt cttccgtgcg ctgacgcagg tagggctggt gctgcgaccg
     5221 tttttaccgg aacaggtcag agcaaaactg cctgctgaaa cggtgaaagc taaaccgcgt
     5281 ccgccgctgc gccataagcg tcgggtttta atgctggaag gctgcgccca gcctacgctt
     5341 tcgcccaaca ccaacgcggc aactgcgcga gtgctggatc gtctggggat cagcgtgacg
     5401 ccagctaacg aagcaggctg ttgtggcgcg gtggactatc atcttaatgc gcaagagaaa
     5461 gggctggcgc gggcgcgcaa taatattgat gcctggtggc ccgcgattga agcaggtgtc
     5521 gaggcaattt tgcaaaccgc cagcggttgc ggcgcgttcg ttaaagagta tgggcagatg
     5581 ctgaaaaacg atgcgttgta tgccgataaa gcgcgtctgg tcagtgaact ggcggtcgat
     5641 ttagtcgaac ttctgcgcga ggaaccgctg gaaaaactgg caattcgcgg cgataaaaaa
     5701 ctggccttcc actgcccgtg taccctacaa catgcgcaaa agctgaacgg cgaagtggaa
     5761 aaagtgttac ttcgtcttgg atttacctta acggacgttc ccgacagcca tctgtgctgc
     5821 ggttcagcgg gaacatatgc gttaacgcat cccgatctgg cacgccagct gcgggataac
     5881 aaaatgaatg cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa cattggttgc
     5941 cagacgcatc tggcgagcgc cggtcgtacc tctgtgcgtc actggattga aattgtagaa
     6001 caagcccttg aaaaggaata acaaaatgaa aactaaagtc attcttagcc agcaaatggc
     6061 gagtgcaatt attgccgcag gtcaggaaga ggcgcagaaa aataactggt ctgtttccat
     6121 tgctgttgcc gatgacggcg gtcatctgct ggcgttaagt cgcatggacg attgcgcgcc
     6181 gattgcggct tacatctccc aggagaaagc gcgtaccgcc gcgctggggc gtcgtgaaac
     6241 taagggctat gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg cgccgttact
     6301 gacgtcgctg gaaggtggcg taccggttgt tgtggatggg caaattattg gtgccgtggg
     6361 cgtttctggt ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag cagcggtgtt
     6421 ggcgaaataa gcgaaaacga ggagataaac aatgagtcaa accataaccc agggccgttt
     6481 acgcattaac gccaatttta aacgttttgt ggatgaagaa gttttaccgg gaacagggtt
     6541 ggacactgcg gcgttctggc gcaattttga tgagatcgtt cacgatctgg caccagaaaa
     6601 tcgtcagttg ctggcagaac gcgatcgcat tcaggcggcg cttgatgagt ggcatcgcag
     6661 caatccgggg ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg aactgggcta
     6721 cctggtgccg caaccggagc gcgtgacggt ggaaaccacg ggcattgaca gcgaaatcac
     6781 cagccaggcg gggccacagc tggtggttcc ggcaatgaac gcccgctacg cgctgaacgc
     6841 ggcgaacgct cgctggggct cactgtacga tgcgttatac ggcagcgaca tcatcccgca
     6901 ggaaggggcg atggtcagcg gctacgatcc gcaacgcggt gagcaggtta tcgcctgggt
     6961 tcggcgtttc ctcgatgaat cactaccgct ggaaaacggc agctatcagg atgtggtggc
     7021 gtttaaggtg gtcgataaac aattacgcat ccagttgaaa aatggtaaag aaaccacgtt
     7081 acgtaccccg gcgcagtttg tcggttaccg tggcgatgcc gctgcgccgt cctgcatttt
     7141 gctgaaaaat aacggcctgc atattgagct acaaatcgat gccaacgggc ggattggcaa
     7201 agacgattcg gcgcacatca acgatgttat cgtcgaagcg gccatcagta ccattctcga
     7261 ctgcgaagat tcggttgcgg cggttgatgc ggaagataaa atcctgctgt accgcaatct
     7321 gctgggtctg atgcagggga ctctgcaaga gaaaatggag aaaaacggtc ggcaaatcgt
     7381 gcgtaaactg aatgacgatc gtcagtacac cgctgccgac ggctctgaaa tttctctgca
     7441 cgggcgttcg ctgctgttta tccgcaacgt gggtcatttg atgaccattc ctgtgatttg
     7501 ggacagcgaa ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga ctggcgcgat
     7561 tgccctctat gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct atattgtgaa
     7621 accgaaaatg cacggtccgc atgaagtggc gttcgccaac aaactgttta cccgcattga
     7681 gacaatgctc ggtatggcac cgaataccct gaaaatgggc attatggatg aagaacgtcg
     7741 gacctcgctg aacttgcgta gctgtatcgc tcaggcgcgt aaccgcgtgg cgttcatcaa
     7801 taccggtttc ctcgaccgta ccggcgatga aatgcattcg gtgatggaag ctggcccgat
     7861 gctgcgtaaa aaccagatga aatcgacgcc gtggatcaaa gcctacgaac gtaataacgt
     7921 gctttccggt ctgttctgtg ggctgcgcgg taaagcgcaa atcggtaaag gcatgtgggc
     7981 aatgccggac ctgatggcag acatgtacag ccagaagggc gaccagctgc gtgccggggc
     8041 aaacacggcc tgggttccgt cgccaactgc cgctacgctc catgcgctgc actaccacca
     8101 aaccaacgta cagagcgtgc aagccaacat tgcccagacc gagttcaatg ctgaatttga
     8161 accgctgctg gacgatctgc tgactattcc ggttgctgaa aacgctaatt ggtcggcgca
     8221 agagatccaa caagagctgg ataacaacgt gcaggggatt ctggggtacg tggtgcgttg
     8281 ggtggagcag gggattggtt gttcaaaagt gccggatatt cacaacgtgg cgttgatgga
     8341 agaccgcgca acgctgcgta tttccagcca gcatatcgcc aactggttac gtcacggtat
     8401 tctgaccaaa gatcaggtgc aggcgtcgct ggagaatatg gcgaaagtgg ttgatcagca
     8461 aaacgctggc gatccggctt atcgtccaat ggtggagaat ttcgctaact cgtgtgcttt
     8521 taaagctgcc tgcgatttga tcttcctcgg agtgaaacag ccaaacggct ataccgaacc
     8581 gttattacac gcatggcgtt tacgcgaaaa agaaaatcat taatagcgtc aaaaaaagcc
     8641 ccggcaagat ttattgtcgg ggctatgata gtaactgcgg tagtacattt atcccggaaa
     8701 taaccggagc gattatcagg ggttaacaat attaaatacc cgtagcgcat cactagcggg
     8761 attaaaacaa caatatggac atcttaacga tactctgcgc ctgcgtgtgc ggaacagaaa
     8821 cccttacccg atatacttgt tatgccagag gtagcaattg cgttagcggc gtactgttgc
     8881 tgtaaagtat aacgcctata ttttacagtt cagtcctctt ttgcttatta caaccttaag
     8941 tatatcaagc atataaagat aataagagac tgaacaatat ggttacctgg acccaaatgt
     9001 atatgccgat gggaggactg gggctatccg ctctggtcgc cctgatccca ataatattct
     9061 tcttcgttgc actcgcggta ttacgtctga aaggacatgt cgctggagca ataaccctta
     9121 tattatctat tctgattgcg atattcgcct ttaaaatgcc gattgatatg gcatttgctg
     9181 ctgcgggcta tggctttatt tatggtttat ggcctattgc gtggattatt gtcgcggcgg
     9241 tattcctgta taaattaacc gttgccagcg gccagttcga tattatccgc agctcggtta
     9301 tctccatcac cgacgatcag cgtttgcagg tgttactgat tggtttctcc tttggtgcgc
     9361 tgctggaagg ggcggctggc tttggcgcac cggtggcgat taccggtgct ctactggtgg
     9421 gcctgggctt caaaccgcta tacgcggcgg ggctgtgtct gattgccaac actgcgccgg
     9481 tggcgtttgg tgcgttgggt gtgccaattc tggtcgccgg tcaggtaacg ggaatcgatc
     9541 cgttcgacat tggcgcaatg gcgggacgtc agttaccgtt cctgtcggtt cttgtgccgt
     9601 tctggctggt agcaatgatg gacggctgga aaggggtgaa agagacgtgg ccagcggcgc
     9661 tggttgcagg gggaagcttc gctgtcactc agttctttac ctctaactat attggtccgg
     9721 aactgccgga tattacttcg gcgctggtga gtatcgtctc actttcttta tttcttaaag
     9781 tctggcggcc aaaaaatacc gaaacggcaa tcagtatggg acaatccgca ggtgcgatgg
     9841 tggtgaataa accgtcttct ggcggtcccg tgccttcaga atatcgtctg gggcaaatca
     9901 ttcgtgcgtg gtcaccgttt ttaatcttaa cggtgttggt caccatctgg accatgaagc
     9961 cgtttaaagc gttatttgct ccgggcggcg cgttttattc gctggtgatt aatttccaga
    10021 tccctcattt gcatcaacaa gtgttgaaag cggcccccat tgtcgcccag ccgacgccaa
    10081 tggacgcggt gtttaaattc gaccccctct ctgctggtgg caccgctatt tttattgcgg
    10141 cgattatctc gatcttcatc ctcggcgtgg ggatcaagaa aggtattggt gtctttgccg
    10201 aaacgctaat tagtctgaag tggccgatac tgtcgattgg catggtgctg gcatttgcct
    10261 tcgtgaccaa ctattccggc atgtccacca cgctggcgct ggtactggct ggtacaggcg
    10321 taatgttccc gtttttctca ccgtttctcg gctggctggg cgtattcctt accggctcgg
    10381 acacctcctc taacgccctg tttggttcac tgcaatcgac cacggcgcag caaatcaacg
    10441 tctctgacac cctgctggtg gcagcaaata ccagcggcgg cgtaactggc aagatgatct
    10501 ccccgcaatc tatcgccgtg gcctgcgccg cgacgggcat ggtgggccga gaatctgaac
    10561 tgttccgcta caccgtgaag cacagtctga tttttgccag cgttatcggc attatcacgc
    10621 tgttgcaggc gtatgtgttt accgggatgt tagtttcgta agtcaataat gccggatgat
    10681 cgttaatatc atccggctac atttacatta ttctgtctta ttaattgaa
//
LOCUS       DAZUCC010000174        10610 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZUCC010000174 DAZUCC010000000
VERSION     DAZUCC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.175"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..72)
                     /locus_tag="ACHY09_003842"
     CDS             complement(<1..72)
                     /locus_tag="ACHY09_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886752.1"
                     /translation="MHTPIGVKPVAGSKEWQEAWQKRA"
     gene            181..675
                     /gene="napF"
                     /locus_tag="ACHY09_003843"
     CDS             181..675
                     /gene="napF"
                     /locus_tag="ACHY09_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFS3886753.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLLFTIGEAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNACGACAASCPVSAITAEY
                     LHVH"
     gene            665..928
                     /gene="napD"
                     /locus_tag="ACHY09_003844"
     CDS             665..928
                     /gene="napD"
                     /locus_tag="ACHY09_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFS3886754.1"
                     /translation="MYTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            925..3411
                     /gene="napA"
                     /locus_tag="ACHY09_003845"
     CDS             925..3411
                     /gene="napA"
                     /locus_tag="ACHY09_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFS3886755.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPLSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3418..4113
                     /gene="napG"
                     /locus_tag="ACHY09_003846"
     CDS             3418..4113
                     /gene="napG"
                     /locus_tag="ACHY09_003846"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFS3886756.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4100..4963
                     /gene="napH"
                     /locus_tag="ACHY09_003847"
     CDS             4100..4963
                     /gene="napH"
                     /locus_tag="ACHY09_003847"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFS3886757.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPASVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4960..5409
                     /gene="napB"
                     /locus_tag="ACHY09_003848"
     CDS             4960..5409
                     /gene="napB"
                     /locus_tag="ACHY09_003848"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFS3886758.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5419..6021
                     /gene="napC"
                     /locus_tag="ACHY09_003849"
     CDS             5419..6021
                     /gene="napC"
                     /locus_tag="ACHY09_003849"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFS3886759.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6034..6657
                     /gene="ccmA"
                     /locus_tag="ACHY09_003850"
     CDS             6034..6657
                     /gene="ccmA"
                     /locus_tag="ACHY09_003850"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFS3886760.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAENKIRRISLTQTRGE"
     gene            6654..7316
                     /gene="ccmB"
                     /locus_tag="ACHY09_003851"
     CDS             6654..7316
                     /gene="ccmB"
                     /locus_tag="ACHY09_003851"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFS3886761.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7358..8095
                     /gene="ccmC"
                     /locus_tag="ACHY09_003852"
     CDS             7358..8095
                     /gene="ccmC"
                     /locus_tag="ACHY09_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFS3886762.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8092..8301
                     /gene="ccmD"
                     /locus_tag="ACHY09_003853"
     CDS             8092..8301
                     /gene="ccmD"
                     /locus_tag="ACHY09_003853"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFS3886763.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVILVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8298..8777
                     /gene="ccmE"
                     /locus_tag="ACHY09_003854"
     CDS             8298..8777
                     /gene="ccmE"
                     /locus_tag="ACHY09_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFS3886764.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8774..>10610
                     /gene="ccmF"
                     /locus_tag="ACHY09_003855"
     CDS             8774..>10610
                     /gene="ccmF"
                     /locus_tag="ACHY09_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFS3886765.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAMLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRNGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRW"
BASE COUNT         2163 a         2723 c         3193 g         2531 t
ORIGIN      
        1 agcccgtttt tgccacgctt cctgccactc ttttgatcct gctacaggtt ttaccccgat
       61 cggggtatgc atctttgaca catcctttaa tatcttagcg gctataaaaa tggcttatta
      121 attatgcggt ttatttggtc gctctcaatt ttcagagcgc gttaatgatg gaaggtcaat
      181 gtgaagattg atgcatcccg tcggggcata ctcactggtc gctggcgcaa agccagtaac
      241 ggtatccgtc cgccctggtc cggtgatgaa tctcattttc tgacccattg cactcgctgc
      301 gacgcctgta ttaatgcttg tgaaaacaac attctgcaac gcggcgcggg tggctatccg
      361 agcgttaatt tcaaaaataa cgagtgcagc ttctgttacg cctgcgcgca ggcttgcccc
      421 gaatcgctat tttctccgcg ccacaccagg gcctgggatt tgctgttcac catcggggag
      481 gcgtgcctgg cgtatcagtc agttgaatgc cgccgctgtc aggatagctg tgagccaatg
      541 gcaattatct ttcgcccgac gctctccggg atctaccagc cgcaacttaa tagccaactt
      601 tgcaacgcat gtggagcatg tgcagccagc tgtccggtat cagccataac tgcggagtat
      661 ctccatgtac actaactggc aagtttgcag cctggtcgtg caggccaaaa gcgaacgaat
      721 ttcagacatc agcacccaac tgaacgcctt tcccggctgt gaagttgctg tcagcgacgc
      781 gccgagcggc cagttgattg tggtggtgga agcagaagac agcgaaacgc tgatccaaac
      841 cattgagtca gtacgcaacg tagagggcgt gctggcggtg tcgctggttt atcaccagca
      901 ggaagagcaa ggtgaggaaa caccatgaaa ctcagtcgtc gtagctttat gaaagctaac
      961 gccgttgcgg ccgctgcggc ggctgccggt ctcagcgtgc cgggcgttgc ccgcgccgtt
     1021 gttggtcagc aggaagccat taaatgggat aaagcgccgt gccgtttctg cggtacgggt
     1081 tgcggcgttc tggtcggaac gcagcagggg cgtgtggtgg cctgtcaggg cgacccggac
     1141 gcaccggtta accgtggtct gaactgcatt aagggctatt tcctgcccaa aatcatgtac
     1201 ggtaaagacc gtttgacgca gccgctgctg cgtatgaaaa acggtaaata tgacaaagaa
     1261 ggcgaattta ccccaatcac ctgggatcaa gccttcgatg tgatggaaga gaaattcaaa
     1321 accgccctga aagaaaaagg gccggaatcg atcggtatgt tcggttctgg tcagtggact
     1381 atctgggaag gttatgccgc gtccaagctg tttaaagcgg gattccgttc gaacaacatc
     1441 gacccgaacg cgcgtcactg tatggcgtcg gcggtagttg gctttatgcg tacctttggt
     1501 atggatgagc cgatgggctg ctatgacgac atcgagcagg ctgacgcgtt tgtgctgtgg
     1561 ggctccaaca tggcggagat gcacccgatc ctctggtcgc gcatcactaa ccgtcgtctc
     1621 tccaaccaga acgtcaccgt ggcggtgctt tctacctacc agcatcgtag cttcgagctg
     1681 gcggataacg gcatcatctt tacgccgcaa tctgacctgg tgatcctgaa ctatatcgcc
     1741 aactatatca ttcaaaacaa tgcgataaat caggacttct tcagcaaaca cgttaacctg
     1801 cgcaaagggg cgacggacat cggctacggt ttacgtccga cccatccgct ggaaaaagca
     1861 gcgaagaatc cgggttctga cgcctccgaa ccgatgagct ttgaagatta caaagccttc
     1921 gttgctgaat atacgctgga aaaaaccgcc gaaatgaccg gcgtgccgaa agaccagtta
     1981 gaacaactgg cgcagctgta tgccgatccg aacaagaaag tcatctccta ctggacgatg
     2041 ggcttcaacc agcatacccg tggtgtgtgg gccaacaacc tggtctacaa cctgcacctg
     2101 ctgaccggca aaatttccca gccgggttgc ggtccgttct ccctgaccgg gcagccttcc
     2161 gcgtgtggta ctgcgcgtga agtgggcacc tttgctcacc gtctgcctgc ggacatggtg
     2221 gtgactaacg agaaacatcg tgatatttgc gagaagaagt ggaatatccc gagtggcacc
     2281 attccggcga aaatcggtct gcacgcggtg gcacaagacc gtgcgctgaa agacggcaag
     2341 ctgaatgttt actggaccat gtgtaccaac aacatgcagg ccgggccgaa cattaatgaa
     2401 gagcgtatgc caggctggcg cgatccgcgc aactttatca tcgtttccga tccgtatccg
     2461 acagtcagtg cgctggcagc ggacttgatc ctgccgaccg caatgtgggt agagaaagag
     2521 ggggcttacg gtaacgccga acgccgtact caattctggc gtcagcaggt acaggcaccg
     2581 ggcgaagcga aatcggatct ctggcagtta gtccagttct cccgccgctt caaaactgaa
     2641 gaagtatggc cggaagagct gctggcgaag aaaccggaac tgcgtggcaa aacgctgtac
     2701 gaagttctgt atgccacccc ggaagtgagc aaattcccgc tatccgaact ggcggaagat
     2761 cagctgaacg atgaatcccg cgagctgggc ttctatctgc aaaaagggct gttcgaagag
     2821 tacgcatggt ttggtcgcgg tcacggtcac gatctcgcgc cgttcgatga ctaccacaaa
     2881 gcacgcggtc tgcgctggcc ggtggtgaac ggtaaagaaa cgcagtggcg ttacagcgaa
     2941 ggtaacgacc cgtacgtgaa agcgggcgaa ggctacaagt tctacggtaa accggatggc
     3001 aaagcggtga tcttcgcgct gccgttcgaa cctgcggcgg aagcaccgga tgaagagtac
     3061 gacctgtggc tctctaccgg acgcgtcctg gagcactggc ataccggcag tatgactcgc
     3121 cgtgtaccgg aactgcaccg cgccttcccg gaagcggtcc tgtttattca cccgctggat
     3181 gcgaaagcgc gcgatctgcg ccgtggcgac aaagtgaaag tggtttctcg ccgtggcgaa
     3241 gtgatctcga ttgtcgaaac gcgcggtcgt aaccgtccgc cgcagggcct ggtgtacatg
     3301 ccgttcttcg atgccgcaca gctggttaac aaactgacgc tggatgcgac cgatccgctc
     3361 tcgaaagaga cggacttcaa gaagtgcgcg gtcaaactgg agaaggtgta agccattatg
     3421 tcccggtcag cgaaacctca aaatggtcgc cgccgctttc tgcgcgatgt cgttcgcaca
     3481 gctggcgggc tggctgccgt gggtgtggcg ctggggttac aacagcaaac cgcacgcgca
     3541 tctggcgtgc ggttgcgccc gcccggagcc ataaacgaga acgcctttgc cagtgcctgt
     3601 gtgcgttgcg gtcagtgtgt tcaggcttgc ccttacgaca ccttaaaact ggcgacgctg
     3661 gcctctggcc tgtcggcggg cacgccatat tttgtcgcac gggatattcc ttgcgaaatg
     3721 tgtgaggaca ttccgtgcgc caaagtgtgc ccgagcggtg cgctggatcg tgagatcgaa
     3781 tcgatcgacg acgcgcgaat ggggctggcg gtactggtgg accaggaaaa ctgtctcaac
     3841 tttcaggggc tgcgctgcga tgtttgttat cgcgaatgcc cgaaaattga tgaggccatc
     3901 accctggagc tggagcgcaa cacgcgtacc ggtaagcacg cccgctttct gccgacggtt
     3961 cacagcgacg cctgtaccgg ttgcggtaag tgcgaaaaag tgtgcgtgct ggaacaaccg
     4021 gcaatcaagg tgttaccgct gtcactagcg aaaggggagt taggtcacca ttaccgcttc
     4081 ggctggctgg aggggaacaa tggcaaatcg taaacgtgac gccgggcgcg aggcgctgga
     4141 gaaaaaaggc tggtggcgca gtcaccgttg gttagtgtta cgtcgccttt gtcagttctt
     4201 cgtgctgggg atgtttttga gcggtccgtg gtttggtgtg tggatcttgc acggcaacta
     4261 cagcagtagc ctgttattcg acaccgttcc gctgaccgat ccgctgatga cgctgcaaag
     4321 tcttgccagc ggtcatctgc cggcctcggt ggcgctgacc ggagcggtga ttatcaccgt
     4381 gctctacgcc ctggcaggaa agcgattatt ttgcagctgg gtctgcccgc tgaacccgat
     4441 taccgactta gcgaactggt tacgcaggcg ctttgacctc aatcagtctg cgacgatccc
     4501 acgccacatt cgctacgtgc tgttggtggt cattctgctg ggatcggcct taaccggcac
     4561 gctcatctgg gaatggatca accccgtttc cctgatgggg cgcagtctgg taatgggatt
     4621 cggcagcggc gcgctgctta ttctcgcgct gtttttattt gatctactgg tcgttgaaca
     4681 cggctggtgc gggcacattt gtccggttgg cgcgctgtat ggcgtgctgg gtagcaaagg
     4741 tgtgattacc gttgcggcca ccgatcggca gaaatgtaac cgctgtatgg attgttttca
     4801 tgtttgcccg gaaccgcatg tgctacgtgc cccggtgctg gatgaacaaa gcccggtgca
     4861 ggtcaccagc cgcgattgca tgacgtgcgg tcgctgcgtg gatgtctgtt ctgaggatgt
     4921 atttacaata actacacgat ggagttcggg agcgaaatca tgaaaagcca tgacctgaag
     4981 aaagcgctgt gtcaatggac ggcgatgctg gccctggtgg tgagcggcgc ggtttgggcg
     5041 gctaatggcg tcgattttag ccagtcgccg gaagtatccg ggacgcagga aggggccatt
     5101 cggatgccaa aagagcagga tcggatgccg ctgaactatg tgaatcagcc gccaatgatc
     5161 ccgcatagcg ttgaaggtta tcaggtaacg accaatacca accgctgctt gcagtgccac
     5221 ggtgtcgaaa gctatcgcac cactggcgcg ccgcgtatca gtcctactca ctttatggac
     5281 agcgacggca aagtgggcgc agaagtggct ccgcgtcgtt atttctgtct gcaatgtcac
     5341 gtaccgcagg ccgataccgc gccaatcgtg gggaatacct ttaccccatc aaaaggttac
     5401 gggaaataag aggttattat gggaaattct gaccgtaagc ctggtctgat taagcgcctg
     5461 tggaaatggt ggcgtacccc cagccgtctg gcgctgggga cgctgctgtt gatcggtttt
     5521 gttggcggca tcgtcttctg gggcggcttt aacaccggga tggaaaaagc caataccgaa
     5581 gagttctgca ttagctgcca cgaaatgcgt aacacggtgt atcaggaata catggattcc
     5641 gtgcactaca acaaccgtag cggcgttcgt gcgacctgtc cggattgtca cgttccgcac
     5701 gagtttgtgc cgaagatgat acgcaagctc aaagcaagta aagagctgta tggtaaaatt
     5761 tttggcgtta ttgacacgcc gcagaaattt gaagctcatc gcctgacgat ggcacagaat
     5821 gagtggcggc gcatgaagga caataactcg caggagtgcc gtaactgtca caacttcgag
     5881 tatatggata caaccgccca gaaatcggtt gccgcgaaga tgcatgacca ggcggtgaaa
     5941 gatgggcaaa cctgtattga ttgccataaa gggatagcgc acaagctgcc cgatatgcgc
     6001 gaagtcgagc caggttttta acagggttat tgcgtgggta tgcttgaagc cagagagtta
     6061 ctttgcgagc gggatgaacg aaccttattt agtggcttgt catttacgct gaacgcagga
     6121 gagtgggtac aaatcaccgg tagcaacggc gcggggaaga caacgcttct ccgtttgctg
     6181 acggggttgt ctcgccctga tgcgggtgac gttctctggc aagggcagcc cttgcatcag
     6241 gtacgcgaca gctaccatca aaacctgtta tggatagggc atcagccggg gatcaaaacc
     6301 cggctgacgg cgttagaaaa tctgcacttt tatcatcgcg atggcgatac cgcacaatgt
     6361 ctggaagccc tggcgcaggc cgggcttgcc ggattcgaag atattccggt aaatcaactt
     6421 tctgccgggc aacaacgccg cgtcgcttta gcgcgtctgt ggctgacccg tgccacgtta
     6481 tggatcctcg acgagccttt taccgcgatt gacgttaacg gcgtcgatcg tctgacccag
     6541 cgtatggcgc agcatacgga gcaggggggg attgtgattc tgactaccca ccagccgctc
     6601 aacgttgctg aaaataaaat tcgccgcatt tcactgacgc aaacgagggg cgaataatgt
     6661 tctggcgcat tttccgtctt gagctgcgcg tagcgtttcg ccatagcgcc gaaatcgcca
     6721 acccgctgtg gttcttcctg attgtcatta ccctttttcc gctcagtatc ggtccggagc
     6781 cgcaactgct ggcgcgtatt gcgccgggca ttatctgggt tgctgcgctg ctttcatcct
     6841 tgctggcact ggagcgactg ttccgtgacg atttgcagga cggcagtctt gaacaattga
     6901 tgttgttgcc gttacccttg cccgccgttg tgctggcgaa ggtgatggcg cactggatgg
     6961 taaccggtct gccgttactc atcctttcgc ccctggtagc aatgctactg ggaatggatg
     7021 tttatggctg gcaagtgatg gcgttaacgc tactgctggg aacccctacg cttggctttc
     7081 ttggtgcgcc gggcgtggcg ctgacagtgg gacttaagcg cggtggtgtg ctgctcagca
     7141 tactggtgtt accgctgact atcccattac tcatctttgc caccgccgcg atggacgcgg
     7201 cttctatgca tttgcccgtt gatgggtatc tggcaatttt aggcgcgttg ctggcaggca
     7261 ccgcgacatt aagtcctttt gcgacggcgg cagcgttacg aatcagcatt caataacgct
     7321 ggattactga tttttgttcg agtctggtat cgaaactatg tggaaaacac tgcatcaact
     7381 ggcgatccca ccacggctgt atcaaatctg tggctggttt ataccgtggc tggcaattgc
     7441 cagtgtggtc gtgcttaccg tcggctggat ctggggattc ggttttgctc cggctgatta
     7501 tcagcaggga aatagctacc gcattatcta cctgcatgtg cctgcggcga tctggtcgat
     7561 gggcatttat gcatcaatgg cggtggcggc gtttattggc cttgtctggc agatgaaaat
     7621 ggccaacctg gcggtggcgg cgatggcccc cattggtgcc gtgtttacct ttattgccct
     7681 ggttaccggc tctgcatggg gaaaaccgat gtggggcacc tggtgggtat gggatgcacg
     7741 cctgacttct gaactggtac tgctgttttt gtatgtgggt gtgattgccc tgtggcacgc
     7801 cttcgacgac cgccgtctgg cgggccgtgc ggcaggtatt ctggtgctga ttggcgtggt
     7861 gaatctgccg attattcatt actccgtgga gtggtggaac accctgcatc agggatcaac
     7921 gcggatgcaa caaagtatcg atccggcgat gcgttcgccg ctgcgctggt cgatttttgg
     7981 cttcctgctg ctgtctgcca cgctgacgct gatgcggatg cgtaatttga ttttgctgat
     8041 ggaaaaacgc cgtccgtggg tgagtgaact gatactgaaa agaggccgta aatgacccct
     8101 gcatttgctt cctggaatga atttttcgca atgggcggtt acgccttttt tgtctggctg
     8161 gcggtggtga tgaccgttat tccgctggtg attttggtcg tgcactcggt gatgcagcat
     8221 cgcgcaattc tgcgtggcgt ggcgcaacag cgggcgcgtg aggcgcgttt acgtgctgcg
     8281 caacagcagg aggctgcatg aatattcgcc gtaaaaaccg cttgtggatt gcctgtgccg
     8341 tgctggcggg gctggcgctg actatcggtc tggtgctata tgcgctgcgc tcgaatatcg
     8401 atctctttta tacgccgggg gagattctct acggcaagcg tgaaactcag cagatgccgg
     8461 aagtcggtca gcgtctgcgc gttggcggga tggtgatgcc ggggagtgtg cagcgcgatc
     8521 ccaattcgct aaaagtaact ttcaccattt acgacgctga aggttcagta gatgtctctt
     8581 acgaaggcat tttgccggat ctgttccgtg aagggcaggg cgttgtggtg cagggcgagc
     8641 tggaaaaagg caatcatatc ctcgcgaaag aagtgctggc gaaacacgat gaaaactaca
     8701 cgccgccaga agttgagaaa gcgatggaag ctaaccaccg tcgcccggcg agtgtttata
     8761 aggacccagc atcatgatgc cagaaattgg taacgggctg ctgtgtctgg cgctaggaat
     8821 tgcgctgctg ctgtctgtgt atccgctatg gggcgtggcg cgcggggacg cgcgcatgat
     8881 ggcgtcttcc cgcttgtttg cctggctgct gtttatgtct gtggctggcg catttctggt
     8941 gctggtcaat gctttcgtgg tcaacgactt taccgtcacc tatgttgcca gcaactccaa
     9001 tacccagctt ccggtgtggt atcgcgtggc ggctacctgg ggcgcgcatg aaggctcgct
     9061 cctgctgtgg gtgctactga tgagcggctg gacctttgcg gtagcgattt ttagtcagcg
     9121 tattccgctg gatattgtgg cccgcgtact ggcgataatg gggatggtca gtgtcggctt
     9181 tttgctgttc attctcttta cctctaaccc gttctcacgc acgctgccga atttcccgat
     9241 tgaagggcgc gatcttaacc cgctgttgca ggatccgggg ctgatcttcc atccgcctct
     9301 gctctatatg gggtacgtcg gtttctcggt ggcgtttgct ttcgccattg cttctttgtt
     9361 gagtgggcgt ctggacagca cctatgcgcg ttttacccgc ccgtggacgc tggcggcgtg
     9421 gatcttcttg acgctcggca ttgtgctcgg ttccgcatgg gcctattacg aactcggctg
     9481 gggcggctgg tggttctggg atccggtaga aaacgcctcg tttatgccgt ggctggtggg
     9541 gactgcgctg atgcactcac tggcggtcac tgaacaacgc gccagcttca aagcgtggac
     9601 attactgctg gcaatcagtg ccttctcgtt gtgtctgttg gggactttcc tcgtgcgttc
     9661 cggcgtgctg gtatcggtac acgcgtttgc gtctgatccg gcgcgcggta tgtttatcct
     9721 cgcctttatg gtgttggtga ttggcggttc gctgctgctg tttgccgcgc gtggacacaa
     9781 agttcgctca cgcgtaaaca atgcgctgtg gtcgcgggaa tctctgctgt tagcgaacaa
     9841 cgttttgctg gtcgccgcga tgctggtggt attactgggg acgctgctgc cgctggtgca
     9901 caagcaactg ggactgggca gtatttcgat tggcgaaccg ttcttcaaca ccatgtttac
     9961 ctggctgatg gtgccgtttg cgctgctgct tggtgtcggt cctctggtgc gctgggggcg
    10021 cgatcgccca cgtaaaatcc gcaatttatt gattatcgcc ttcatctcaa cgctggtgct
    10081 gtcgctgctg ctgccgtggc tgttcgaaag caaagttgtg gcgatgacgg tgctcggcct
    10141 ggcaatggcc tgctggattg cgatgctggc aattgcggaa gctgcgctgc gtatttcacg
    10201 tggcacgaaa accaccttca gttattgggg aatggtggcg gctcacctgg ggctggcagt
    10261 gacaattgtt ggtattgcct ttagccagaa ctatagcgtt gagcgtgatg tgcgcatgaa
    10321 gtccggcgat agcgtcgata ttcatgaata tcgcttcacc ttccgtgatg tcaaagaggt
    10381 gactggtccg aactggcgtg gcggtgtggc gactatcggc gtaacgcgaa atggcaagcc
    10441 ggaaacagtg ctgtatgcgg aaaaacgtta ttacaacact gccgggtcga tgatgaccga
    10501 agcggcaatt gacggcggca tcacgcgtga cctgtacgcg gccctcggtg aagagctgga
    10561 aaacggcgcg tgggccgtgc gtctttacta caaaccgttt gttcgctgga
//
LOCUS       DAZUCC010000175        10562 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZUCC010000175 DAZUCC010000000
VERSION     DAZUCC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.176"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            128..316
                     /locus_tag="ACHY09_003856"
     CDS             128..316
                     /locus_tag="ACHY09_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886766.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTRAVISGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            313..1140
                     /locus_tag="ACHY09_003857"
     CDS             313..1140
                     /locus_tag="ACHY09_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS3886767.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDSALKDIQAEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1181..1552
                     /locus_tag="ACHY09_003858"
     CDS             1181..1552
                     /locus_tag="ACHY09_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS3886768.1"
                     /translation="MNTHNAQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVISCAIESLYATLPYEEVEDERTGGTDIMATINIGELICQDEDLSGESW
                     LAGMLVAAEIISIEPATNIRL"
     gene            1584..1826
                     /locus_tag="ACHY09_003859"
     CDS             1584..1826
                     /locus_tag="ACHY09_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS3886769.1"
                     /translation="MFDLINQGQLYTDSAGYPVKIIHCINNIVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            1830..1964
                     /locus_tag="ACHY09_003860"
     CDS             1830..1964
                     /locus_tag="ACHY09_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886770.1"
                     /translation="MNKAFELWVRQCYGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            1983..2237
                     /gene="xisR"
                     /locus_tag="ACHY09_003861"
     CDS             1983..2237
                     /gene="xisR"
                     /locus_tag="ACHY09_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFS3886771.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGVKNASSG"
     gene            2271..3557
                     /locus_tag="ACHY09_003862"
     CDS             2271..3557
                     /locus_tag="ACHY09_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3886772.1"
                     /translation="MSNTSYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGNFDYAAQFPESPNLKHFGLGKKEITIKALSEKWLDLKKIEIGGN
                     AFNRYQSVVRNMLPRIGESRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPVKGRSVV
                     TVNYYMTTIAGMFQFAADNGYTESNPFNGLTPLKRSRTEPDPLTHDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAIETLKSQAEMTRFGTQYQIDVKLREFGRTVSHECTFVFNPQLVKKCQQAGY
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALAAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKELAESVPYMPHRARR"
     gene            3559..4338
                     /gene="yecE"
                     /locus_tag="ACHY09_003863"
     CDS             3559..4338
                     /gene="yecE"
                     /locus_tag="ACHY09_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS3886773.1"
                     /translation="MISFSIIELTHGSRHVNCVEGNTTLYALPKPEVVLRWREQTTDD
                     FRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALW
                     HFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAARPHSE
                     AIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYL
                     FLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            4391..4786
                     /gene="yecN"
                     /locus_tag="ACHY09_003864"
     CDS             4391..4786
                     /gene="yecN"
                     /locus_tag="ACHY09_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFS3886774.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            4827..5570
                     /gene="cmoA"
                     /locus_tag="ACHY09_003865"
     CDS             4827..5570
                     /gene="cmoA"
                     /locus_tag="ACHY09_003865"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFS3886775.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            5567..6538
                     /gene="cmoB"
                     /locus_tag="ACHY09_003866"
     CDS             5567..6538
                     /gene="cmoB"
                     /locus_tag="ACHY09_003866"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFS3886776.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(6703..9132)
                     /gene="torZ"
                     /locus_tag="ACHY09_003867"
     CDS             complement(6703..9132)
                     /gene="torZ"
                     /locus_tag="ACHY09_003867"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFS3886777.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(9157..10257)
                     /gene="torY"
                     /locus_tag="ACHY09_003868"
     CDS             complement(9157..10257)
                     /gene="torY"
                     /locus_tag="ACHY09_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFS3886778.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPIASDHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2718 a         2610 c         2510 g         2724 t
ORIGIN      
        1 aaagcgaccg tgaattaagc agagtaaaac tgcttttaat ccttgccaca gtactgacac
       61 taacagaaat cattattctc tttattgcgc tgtctgtcag ttaaaaatat cgggatacca
      121 cataccaatg agactgtatt tcacaatagt aattttactg gcaattatcg catgcattta
      181 cggattactc gttccgttcc ttatatccat gaaggatacg agagcagtta tttctggctt
      241 tgcactggcg tttctgaccc cgccctgcat ttatgccatt tacaagggtc tttctttcac
      301 taaggataaa aaatgaaaaa aattattttt gctttagcca ttgttctgcc gaccattggt
      361 cttgtcggtt gcgatcgcgt tgagcccggt aatgttggca tcaaagtaaa taaactgggc
      421 gacgataaag gcgtcggtga ggtggtcggt gttggtcgct actggactgg ctggaatact
      481 gaagtttaca tcttccccac cttcaagcaa atgaagacct acgatgaacc gttcagcttt
      541 cagatgagtg acggcacaac catcggctat cacatcggtg tggcctacaa ggttgatcca
      601 tccaaagtta ctacagtgtt tcaaacctac cgcaaaggcg tggatgacat taccgacact
      661 gacctgcgcc agaagatagc cgacgcactc aaccgactgg ccagcaaaat gaccaccgat
      721 aaatttatcg acggtggcaa gtctgaactg ctggattcag cacttaaaga tattcaggca
      781 gaaatgacac ccatcggcat tcaggtaatg agcctctctt atgtcggtaa accggaatac
      841 ccgccaaccg tgatcgacag cattaatgcc aaagtcacag cgaaccagaa aaccctgcaa
      901 cgcgaacagg aagtaaaaca gcgcgaagcg gaagccaaca tgttgcgcgc ggaagcagcc
      961 ggacaggctg acgccattcg cacaaaagcc caggccgaag ccgatgctat tcgtttacgc
     1021 ggtgaagctc tgcgccagaa ccccggtgtt atggagctgg aagccatcaa taaatggaac
     1081 ggcacactgc cgcaatacat gaccagcggt gccaatacac catttatcca ggttaaataa
     1141 cttatatgcc cggcaggccg ccgggctaag ggaaaagcag atgaacaccc ataatgccca
     1201 accgcaaata atgaactatg acccgaatct gacgtcatgc ggacgcatgg caaaacaaac
     1261 cgttcgatta actttcgggt tatgggaata ccgcgaaaca ttcgaagtta ctgtcggcgg
     1321 caatctgacc ggactggatg ttatcagttg cgctattgaa agcctgtacg caacactgcc
     1381 ttatgaagaa gtcgaggatg agcgcacagg gggaacagat atcatggcca ccattaatat
     1441 tggcgaactg atatgtcagg atgaagacct gtccggagag agctggcttg ctggaatgct
     1501 tgtagctgca gaaattatca gtattgaacc cgctacaaat atacggctct gaagttctcg
     1561 ctattcagag aacaggagaa aaaatgttcg atttgattaa tcaaggacaa ctgtataccg
     1621 acagcgccgg ttacccggta aaaattattc actgcataaa taacatcgtg ttgtacagaa
     1681 gaatggatgg acgaacacag tcggtaaaaa taaacgattt taatgaactg tttgaacgaa
     1741 tcgatcacca ggaataccga caaattctgg cagaaacaga acaagaagct catctgaaaa
     1801 aattacgtgc catgaaaagg aagtaaagaa tgaataaagc gtttgagcta tgggtacgcc
     1861 agtgttacgg caatcgctat gacctgacgc gagatgttga cggtttctac tgccgtgaag
     1921 ttgtgaaacg aatgtttgac gtgtggtgcc actgccgtgg atgaaaattt tatgaggttg
     1981 gcatgcagac aatcatctat cagataaccc ccagcaaatg gtgtacggag agagtcctca
     2041 ttgcatcaac agggctaaag cctggcacca ttgagcgggc aagaagaaag tcatggatgc
     2101 agggaaaaga ataccgccat tacgctgtag aaggtgatcc agggcattac agtgaatgcc
     2161 tgtacaacat cgaagaaatt atgcgatgga tcgaaaacca gaaacaacca ggtgtcaaaa
     2221 atgcaagttc cggttaacct gttaatgctc ctggacgtct gggaggttca atgagtaaca
     2281 catcataccc gacaggcgtt gaaaaccatg gcggatcact ccgcatatgg tttcactata
     2341 acggcaaacg tgtcagagaa aacctcggtg ttcctgacac agccaaaaac cggaagattg
     2401 caggcgaact caggacttca gtttgttttg caatcagaat ggggaatttc gactacgctg
     2461 cgcagtttcc tgagtcccca aatctgaaac actttggtct gggaaaaaag gagataacca
     2521 ttaaggcgct ttcagaaaaa tggctggatc tgaaaaaaat cgaaatagga ggtaacgcat
     2581 tcaatcggta tcaatctgtg gtgagaaaca tgttgcctcg tataggggaa agccgactgg
     2641 cctcatctgt aacaaaagaa gatttactct tcatcaggaa agatttattg accgggtatc
     2701 acaatctctc aaacggcaaa acaacacctg ttaaggggag atctgtagtt acagttaatt
     2761 actacatgac gacaattgca ggtatgtttc aatttgcagc tgacaacggc tacacggaat
     2821 caaacccgtt taacggcctg acaccattaa aaaggtccag aaccgagcca gatccgctca
     2881 cgcatgacga attcattcgt tttattgatg cttgccacca tcaacaaacg aaaaacctgt
     2941 ggtctttagc agtatacaca ggcatccgtc acggtgagct aatatctctc gcctgggagg
     3001 atattgattt aaaagctaaa acaatgacta tccgtcgtaa ttatacaaaa ctcggggagt
     3061 ttactctacc aaaaaccgaa gcgggaacag atcgtgttat tcatcttgtt caaccagcca
     3121 ttgaaaccct gaaaagtcag gctgaaatga caagatttgg cactcaatac cagattgacg
     3181 tcaaactgcg ggagttcgga cgcaccgtaa gccatgaatg cacgtttgtc tttaatccgc
     3241 aactggtgaa aaagtgccag caagccggtt accactacaa agcggactcc atcagggatt
     3301 catgggcatc agccctgaga cgggccggac tacggcacag gaaagcctat cagtcaaggc
     3361 atacttatgc ctgctgggca ctggccgcag gagcgaatcc aagctttatc gcaaaccaga
     3421 tggggcacgc aaacgctcag atggtattca atgtttacgg agcatggatg aaagataaca
     3481 atatcgggca gatagaactg ctcaataagg agttagctga aagtgtccca tacatgcccc
     3541 acagagcaag acgctgaaat aatttctttc agtatcattg agttaaccca tggttcccgc
     3601 catgttaact gcgtggaggg taacaccacg ctttatgccc tgccgaaacc cgaggttgtc
     3661 ctgcgctggc gtgagcagac cacagatgac ttccgcttct gttttaagtt tccggcgacc
     3721 atttcgcatc aggcagcatt acggcattgc gatgatttag tgactgaatt tttgacccgc
     3781 atgtcaccgt tggctccgcg cattggacaa tactggctgc aactgcctgc cacattcggc
     3841 ccacgagagc tgcctgcgct ttggcatttt ctcgattctc ttcccggtga atttaattat
     3901 ggggtggaag tccgccatcc acagtttttc gccaaagggg aagaggaaca aacgcttaat
     3961 cgcggtttac atcagcgcgg cgttaatcgg gtgattttag acagccgccc ggttcatgca
     4021 gcacgtccac acagtgaagc tattcgcgac gctcaacgaa aaaaacctaa agttccggta
     4081 catgctgtac tgacggcgaa aaatccactg atccgtttta tcggtagtga tgatatgacg
     4141 caaaaccggg agttatttca ggtctggtta caaaaattag cacagtggca tcagaccact
     4201 acgccttatc tttttttaca tacgccagat attgcccagg ccccggaact ggtacatacc
     4261 ctgtgggaag acttacgtaa aacgcttcca gagatcggag cagttccggc tattccacag
     4321 caatcttctc ttttctgaat ttgccaccta tcatagacag gtgccatcgg ccattttaaa
     4381 gggagtttgt atggtaagcg cgctgtatgc cgttttaagt gcgttgttat taatgaagtt
     4441 ctcttttgat gtcgttcgcc tgcgaatgca gtaccgcgtt gcctatggcg acggcggttt
     4501 tagcgaactg caaagcgcta ttcgcattca tggtaacgcg gtggaatata ttcctatcgc
     4561 gattgtgttg atgctgttta tggaaatgaa tggcgcagaa acctggatgg tgcatatttg
     4621 cggcatcgtt ttgcttgctg gtcgtctgat gcattattac ggttttcatc accgtctgtt
     4681 ccgctggcga cgttctggca tgagcgcgac ctggtgtgcg ctgttgctga tggtgctggc
     4741 gaatctttgg tatatgccct gggagttggt tttctccctg cgttagcgca caatacgcca
     4801 ctttcttttt cccggatttt tacgttatgt ctcaccgcga cacgctattt tctgccccta
     4861 tcgccagact gggcgactgg acctttgatg aacgggtagc tgaagtcttc ccggatatga
     4921 tccagcgttc cgttcccggc tattccaata ttatttccat gattggtatg ttagccgagc
     4981 gcttcgttca acctggtacg caggtttacg atctgggttg ttctctgggc gcggcgacgc
     5041 tctcggtgcg tcgcaacatt catcatgata attgcaaaat tattgccatc gataactccc
     5101 ccgcgatgat tgaacgctgc cgtcgtcata ttgacgccta taaagcccct acgccagtag
     5161 acgtcattga aggtgatatt cgcgatatcg ccattgaaaa cgcatcgatg gtggtgctga
     5221 attttaccct gcaattcctg gaaccttccg agcgccaggc gttactggat aaaatttatc
     5281 aagggctgaa cccgggcggc gcgctggtgc tttcggaaaa attcagtttc gaagatgcca
     5341 aagttggtga actgttgttc aacatgcacc acgactttaa acgtgccaac ggttacagcg
     5401 aactggagat cagccagaag cgcagcatgc tggaaaacgt gatgctgacc gattccgtgg
     5461 aaacccataa agcacgcctg cataaagctg gttttgaaca tagtgagctg tggttccagt
     5521 gctttaactt tggttcactg gtggcattaa aagcagagga cgctgcatga tcgactttgg
     5581 taacttttat tctctgattg ccaaaaatca tctttcacac tggctggaaa cgctgcccgc
     5641 gcagattgct aactggcagc gcgagcagca gcacgggctg tttaagcagt ggtccaacgc
     5701 ggtggaattt ctgcctgaaa ttaaaccgta tcgtctggat ttattgcata gcgtaaccgc
     5761 cgaaagcgaa gagccactga gcgccgggca aattaaacgc attgaaacgc tgatgcgcaa
     5821 cctgatgccg tggcgcaaag ggccgttctc actgtatggc gtcaacatcg ataccgaatg
     5881 gcgttccgac tggaaatggg atcgcgttct gccccatctt tctgatttaa ccgggcgcac
     5941 cattcttgat gtcggctgtg gcagcggtta tcacatgtgg cgcatgattg gcgcaggcgc
     6001 gcacctcgcg gtgggaatcg accctacgca actgtttctg tgccagtttg aagcggtgcg
     6061 taaactactg ggtaacgatc agcgcgcaca tttgttaccg ttaggtattg aacagcttcc
     6121 ggcattgaaa gcctttgata ccgtcttttc gatgggcgtg ctttatcatc gtcgttcacc
     6181 gctggagcat ctctggcagt taaaagacca actggtgaat gaaggtgaac tggtgctgga
     6241 aacgctggtt attgatggtg atgagaacac ggtgctggtg ccgggcgatc gttacgcgca
     6301 aatgcgtaat gtctatttca tcccttccgc gctggcgctg aaaaactggc tgaagaagtg
     6361 tggttttgta gatatccgca ttgcggatgt gagcgttacc accacagaag agcagcgacg
     6421 caccgaatgg atggtcaccg agtctctggc cgattttctc gacccgcatg atccgggtaa
     6481 aacggtggaa ggttatcctg cgcctaaacg cgcggtgctg attgcgcgca agccgtaaag
     6541 gtctggtatt actgccggat gcggcgtgaa cgccttatcc ggcctacaaa accttgctaa
     6601 ttcaatatat tgcagggact atgtaggcct tataagcata gcgcatcagg cagttttacg
     6661 tttgcataac ctcagcgccc gtttccgggc gcttttcacg tcttatgcct gaacagctgg
     6721 ctgatcaaac gccgttaact tcggtgcatt gcccgtatat ttttcgatat acaccagcgc
     6781 agagttaccg gcacaggcat ttgccagctg agagctgggg atatccgccg ttaacacgtt
     6841 cgcactgccg tttttacaca agccattttc cagatctggc catgcacctt catgcacgca
     6901 taccacgcct tttttgatcc cgtcagtcac caccgcgcct gtcagaatct gaccacgttt
     6961 gttccagaca cgcaccagat cgccattcgc aataccaaag cgagcagcat cttcggtgtg
     7021 aatagtgatc ggttcacgat cggcaaccgc atatttttta cgcagttccg catagttaag
     7081 ctgactgtgt aaacggtgtg ccggatgtgc ggtcagaagc tgcaactgct tctcgtcggc
     7141 ggaacctttc cactcatcag gcgcaagcca ggttgggtgt gccgggcaat ccttatagcc
     7201 aaatttttcc agcgttttgg agtaaatctc aattttgccg cttggcgtac ccagcgcatt
     7261 tttcaccgga tcggcgcgga aatcaccata acgaacgtat tgttcgttct tctcactgcg
     7321 gcgcatttcg atcagtttat tttgctgcca gaaagcatta aacatcggca tagtcacgcg
     7381 ttgcgcacgg gcacctttct gagcggcatc atagaaaaat ttcagccacg ccatttcatc
     7441 tttaccttcg gtatagatct cttttccgcc aggtttgagt aattcagcaa gatcggcaaa
     7501 cacgtcaaaa tcgttgcgcg cttcaaattg cggagcgaca gcctgcttca tcggcacaat
     7561 atgctggttg ctgtaatcac cggtcatcgt caggtcattg cgctcaaacg atgtggtgat
     7621 cggtaatacg atatctgcat gtttagcagc tgcggtccag tagcattcag aaacgacgat
     7681 catctccggt ttctgccatg ctttaatcag acgattagta tcctggtgat gggtaaagtt
     7741 accgccaccc gcccaccaga tcattttgat attcggatac gtttgttctt taccgttatg
     7801 ctgatatttg ccgccaggat tttccagcgc atcgacgata cgggcaacag gaatcgccgt
     7861 cattccgcca tcatcagcgg cctcgctggc ctgtccggca atcgccgcag acatttccgg
     7921 cagcacgccg ccaacacgcg tcgggttgcc accgttggag taatgataag agaatccaaa
     7981 accgccgccc ggtgtaccaa tttgccctaa catcgccgcc agcgtcacca gcatccagtg
     8041 tttctgttca ccgtattgct ggcgttggat cccccagcct gccatcagca tcgtgcgatt
     8101 cgccgccatc agttctgcaa gtttgacaat ctgcgcttcc ggtacgccag taatttccgc
     8161 cgcccaggcc gcgctcttcg gcgtattatc gctcttacct gtcagatact cttcaaactg
     8221 cggatacccg gtagtgtatt tttcaaggaa cactttatcg tgtttgcctt gtgtcatcag
     8281 ggtatgcgca atccctaaca tcagtgccac gtcggtgccc atattcggtg cgatccaggt
     8341 ggcattatcg ccaaagaatt cgatggtttc ggagcagata ggatcaatgg caatcactgg
     8401 tttgccagat tttttcagct gatggaagta ttccagccct tgctcatcgg tactgctcca
     8461 ggcaattttg agcgtgttca gcgggttcat cccccacagc accacaacct ggctgttttc
     8521 cagaatcagc ggccaggagg tctgctgttc atacacctct acagaaccga ccacatgcgg
     8581 catgatcacc tgtgccgctc cggttgaata atcgccgcta tgcccggaat aaccgcccgc
     8641 caggttcata taacgttgca gtaaggtttg cgctttatgc aacacgccag aagaacgcca
     8701 gccgtaagat ccggcaaaaa tggccgatgg tccgttagct ttacgaatac gatcgtgttg
     8761 ctcatgaatc agttttaatg cttgttccca gctcacctgt acataggtat cttcgccacg
     8821 acctttcgcc ggttgcagtg gattatcgag atagcttttt ctcaccatcg gatgctgaat
     8881 acgcgccgtg gtgtgtacct gatccgccgc cgtagactgt aaggagttcg gtatggtttt
     8941 cgccagcgcg cctgtcgaag aaacaatctt gccgtccttc acttctacgt tcatcgctcc
     9001 ccaacggccc gcggtgagaa ttttgccgcc cttctcttct gcccatgcag gtaacggtgc
     9061 agccgatgtc accaccagcg cccctgccgc aataccgcta tgtttaataa attcacgtct
     9121 tgttaatgtc ataacttcct ccctgatcaa cgaggatcac tgtttctcgg taatatcttt
     9181 ggcgttgtac tggaagtacc gcgtcaaaat gtccagttcg ttttcgctca tgctggttcg
     9241 cgcacccatt cctttggcaa tggacggcca tgcattgacg gtgtaatggt cggaggcaat
     9301 aggggcatgg caaccagcgc aataggtatc gtcaagtttt tcagcgtatt gccatagagg
     9361 tttacggtcc gctaatgcgg gatcggtaag cacaccctgt aaagacgcct gacgccattg
     9421 attgccgtat tcgtcagcct gccattcccc gtttaccgtc agcgccttga taccttcttc
     9481 acttaatgtg gctagcgcca gccgttgacc tgccgccagg tagagcgtgt tttcactgcc
     9541 ctgcatttga taaccctgca acagaacgat cggctgtttg ccactggcat caacgacggt
     9601 gagatcggta ccaggattca cggtagccag ttcgcctatg cgagaagttt tgaaaggata
     9661 aatatgtgcg ccattagtaa ctgaagtagc ggcctgactt tccagctcat gcgccgcgtt
     9721 gtcatccatt tttatttctg gcggaaaatg ggcaatgcct ttatggcaat cgatacaggt
     9781 ttcgccgtcc ttttgcgctt tattatgcat tttctgcgca gattcacttt gcgaggcaat
     9841 atccatggca tcaaaagaat ggcaactacg gcaggttgca gagtcagtgg cttttaattc
     9901 tttccatact gtttcggcca tttcctggcg atgagcttcg aacttatcat cactgtctat
     9961 tttgccgcta acaaattcat gataaatatc tttagatgct tttaatttag caaataaata
    10021 atccatccct gactttggaa tatggcaatc ggcacattcc gcacgtatcc ctttctggtt
    10081 tgaaaagtgg acagttccct gatattcctc aaaaggttta ctcatcgagt ggcaggaaag
    10141 gcaaaatgcg gtatccgacg ttttatgtaa ggctttttgc gccagtaata acccgccgcc
    10201 accaaccaca accgctatca gcaaaaacaa taacccaatg cgttttttcc ctcgcataga
    10261 ttcctctaat taaattccag gacaccgata ccattgtata aataaataca caacgaattg
    10321 actacttgct taattcggca caacactgcc tgaaataatc gataaagaat atgatttatt
    10381 acaatgtaat cattaattgc taaggaataa cctagttgtt atttagaata tatatttgtc
    10441 agttttggca atttaaatca cataatgaat gtgaatatat aaaatatttt tagcacaacg
    10501 ctatgcgcac tgtcacccgc tcaacagatc acggcaatcg ttcgtttttt atactgctca
    10561 ga
//
LOCUS       DAZUCC010000176        10391 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZUCC010000176 DAZUCC010000000
VERSION     DAZUCC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.177"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            59..256
                     /locus_tag="ACHY09_003869"
     CDS             59..256
                     /locus_tag="ACHY09_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3886779.1"
                     /translation="MSNNLLRLSDVMRRTGYGRAWIYRLINQGRFPKPVKIGPRSVAF
                     IESEVDEWINQRIEASRNNAA"
     gene            337..1113
                     /locus_tag="ACHY09_003870"
     CDS             337..1113
                     /locus_tag="ACHY09_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS3886780.1"
                     /translation="MPVQGQGFVQPEELSQQYFAAIIPVISGNEIIADGINKNALTAP
                     EVFPSGGEPVTTSQAIARFFRKDHDKVVNRIENLCCSAEFLTRNFRRVKFEHKGRVLK
                     AYEVTKDGFVFLTMGFTGKKAAAFKEAYIAQFNRMANWIAERCEAKAEMRSMGDAMKG
                     YIERTGDTQHGHAYNNECTFIARLVLGMHPPVWAKKNGIPSKKVRDHMTADQLALLAY
                     LESRNCALLDMDMPTPERKERLTELAQRWLVRRLGEREHA"
     gene            1106..3007
                     /locus_tag="ACHY09_003871"
     CDS             1106..3007
                     /locus_tag="ACHY09_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFS3886781.1"
                     /translation="MPDILKPAVRYIDTADKFALLNINHADYMRHIVSHFTAERMKHV
                     KQYLEEGLSNYRAGGPVRREYPHDAGMAGKTKATTKGSPVEIKEIKRIIHNAISEAGG
                     QYAVYDEWPLGPDVRIFLRHVLFRKLSSAKASGASLCSRARALSSLRVNSVALACSRA
                     AFRFRSCSVIRALVSVTIMAKEFFFWSSISYPLLLDRLECCFKKSSSAKLVLKVFSNR
                     LRISISAMCLVIDCFFSTKCCSYAVRSSFLFSDCEVCCVLSILCTCKTMRRTSSHHGA
                     DGFCLFHRAAEETASYQPGGRFLVAFNRRGYFSTKASRANRVGLVSGCGLSNNLLCRC
                     AFSRCRSSMDNAISCSSSFVIVCTCKAIRRNTPRYSDDGFCCCNLLRWFFLPLRNTSS
                     GGGSLVFSGGVNFSFLVSMMSRISRWRCSALIFGVTGENLCLLILDSGNALCRDASYH
                     GAGYCLVRCLSFWYPSFLEGYVNIFRTNFSRNTSDHLSKKAAYTEAIPYKTGIGIGLP
                     LELEAIHDAPASFFVSAHTHTSTMVGCMELPSGRLVSSNTSKANSVQSTASKIGLLGG
                     GYNPKVRGAFHMAVSARPYFVWRFMQCLTDSIAIFTVTAATEREARAQLPHAHLIFVA
                     RIRQEVTHA"
     gene            3000..3185
                     /locus_tag="ACHY09_003872"
     CDS             3000..3185
                     /locus_tag="ACHY09_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886782.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            3189..3383
                     /locus_tag="ACHY09_003873"
     CDS             3189..3383
                     /locus_tag="ACHY09_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886783.1"
                     /translation="MCNFSDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDMQ"
     gene            3450..3563
                     /locus_tag="ACHY09_003874"
                     /pseudo
     CDS             3450..3563
                     /locus_tag="ACHY09_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            3569..5389
                     /locus_tag="ACHY09_003875"
     CDS             3569..5389
                     /locus_tag="ACHY09_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFS3886784.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWLEQDGKLA
                     GDNIPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHSERQNWRNYLDIERKNISDGLVIELPVKKKARGQGDDSSLALNQMGASQ
                     RGEVLLAHYGGELAINADSDTVHYYNSVVWVPVQDKELQRTMAQIFIDEDICYSQNAI
                     KSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFTPPAE
                     GETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKS
                     VMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAITG
                     GDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENERD
                     PMLPEKIEGELAVVIRHLLTRFSDQDEARLLLHEQQKSEEALAIKREGDSLVDFCGYL
                     MSSVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFSKPVTLTRFGADMPGAMAEY
                     GREYMKRKTKHGLRSNVTLTEESEDWMPSCAPVKNEGDKN"
     gene            5676..6125
                     /locus_tag="ACHY09_003876"
     CDS             5676..6125
                     /locus_tag="ACHY09_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886785.1"
                     /translation="MTEKHAKKSHSHAIDLTEPESVVRFVSVRGRATLYIPDEHLHHC
                     DKHKLPILIVWKRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERAGKIYEKYAANS
                     KRAARAITYHFMTLYDLKADDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            6122..6571
                     /locus_tag="ACHY09_003877"
     CDS             6122..6571
                     /locus_tag="ACHY09_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS3886786.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTAMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            6588..7034
                     /locus_tag="ACHY09_003878"
     CDS             6588..7034
                     /locus_tag="ACHY09_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFS3886787.1"
                     /translation="MNQQTTQQDQTTRKPSRRIPWQHMTKVQRKNHRRINRIAEYWPE
                     LFGTENIKPLKAGIIRDVLEDAKSRELPLATGVLRGALTSHLHTTRYLKAIIAGGPRY
                     DLNGQPCGEVTEEDKSVASELLKRRLAQIKQQNSQQQTTGDDGDDS"
     gene            7024..7317
                     /locus_tag="ACHY09_003879"
     CDS             7024..7317
                     /locus_tag="ACHY09_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFS3886788.1"
                     /translation="MIADSVAQKLEERGLWRRAATRWGDVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQLVTFGDVKKAADRTLKEMGINAQDEWKNYSFSDAGDDLALP"
     gene            complement(7754..7951)
                     /locus_tag="ACHY09_003880"
     CDS             complement(7754..7951)
                     /locus_tag="ACHY09_003880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886789.1"
                     /translation="MIGFVIGFVIDLRIFLRNFLCIFSGIFPWIFHPTEGASGLPDND
                     QVNRTGKGAGCAGGRAIKNPE"
     gene            8228..8441
                     /locus_tag="ACHY09_003881"
                     /pseudo
     CDS             8228..8441
                     /locus_tag="ACHY09_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9512..9886)
                     /locus_tag="ACHY09_003882"
     CDS             complement(9512..9886)
                     /locus_tag="ACHY09_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886790.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            complement(10194..>10391)
                     /locus_tag="ACHY09_003883"
     CDS             complement(10194..>10391)
                     /locus_tag="ACHY09_003883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886791.1"
                     /translation="GTEQQAMPPAIKPFPHILKSILYTFLYTFSRRLMTYSGKYLPEF
                     FRLSAMKLVDALRYGNPHNDT"
BASE COUNT         2880 a         2361 c         2677 g         2473 t
ORIGIN      
        1 aatatgtttt ccttttatgc atcattgtag acggttatag acgttacgag gattgaatat
       61 gtcaaataac ttacttcgtc tttctgatgt tatgcgccgt actggttacg gtcgcgcttg
      121 gatttatcgc ctgatcaacc aggggcgctt tcctaaacct gtaaaaattg gtccccgctc
      181 cgtggctttt attgaaagcg aagtcgatga atggattaac cagcgtatcg aggcttctcg
      241 caacaacgcc gcgtaataat cttttgggag ggggatattc tcccccagca ctaaacctga
      301 tccgatagca ggagtttttc aatgaaacgt ccgaatatgc ccgtacaggg gcagggcttc
      361 gttcagcccg aagaactgag ccaacagtat ttcgccgcaa ttattccggt aatttccggc
      421 aatgagatta ttgctgacgg aataaataaa aatgcactca ccgcgccgga agtgtttcct
      481 tctggcggtg agcctgtaac cacttcacag gctatcgccc gatttttcag aaaggatcac
      541 gataaggttg ttaaccggat tgaaaacctg tgttgttctg ctgaattttt aacccgcaat
      601 tttcggcggg taaaatttga gcataagggg cgcgtattga aagcctacga agtcaccaaa
      661 gacggcttcg tgtttttaac aatgggattt accggaaaaa aagcggctgc attcaaagag
      721 gcatacattg cgcagtttaa ccgcatggct aactggatag cagagagatg tgaggccaaa
      781 gctgagatgc gctctatggg ggatgccatg aagggataca ttgagcgcac tggagatact
      841 caacacggtc atgcttataa caacgaatgt actttcattg cgcgccttgt attagggatg
      901 cacccgcctg tatgggccaa aaaaaacggc atcccatcaa aaaaagttcg tgaccatatg
      961 accgctgatc aactggcgtt gctcgcgtat ctggaaagcc gtaactgtgc gctactggat
     1021 atggatatgc caacgccaga acgtaaagaa cgtctcaccg aattagccca acgctggctg
     1081 gtccgtcgcc tgggagaacg tgaacatgcc tgacatcctc aaacctgctg tccggtacat
     1141 cgacacggcg gataagttcg cactactgaa catcaaccat gcggattaca tgcgccatat
     1201 cgtgagccat ttcaccgctg agcgcatgaa acacgttaag caatacttgg aggagggttt
     1261 atcaaattat cgcgctggtg gccctgtacg gcgcgaatat ccgcatgatg caggaatggc
     1321 aggcaaaaca aaggcgacca cgaaagggtc gccagtggag ataaaggaaa tcaagcgcat
     1381 tatccacaat gccatatctg aggctggcgg acaatatgca gtttacgacg aatggcccct
     1441 tggtcccgat gttcgtattt tcttacggca tgtacttttc agaaaattat caagtgcaaa
     1501 agcttcaggt gcttctctgt gttcccgtgc cagagcgtta agttctttac gtgttaattc
     1561 tgttgctctt gcctgttcgc gtgcagcttt ccgctttcgt tcatgttcag ttatacgggc
     1621 gctggtcagt gtaaccatca tggcaaagga gttttttttc tggtcatcca taagctatcc
     1681 cttattgctt gatcggctgg aatgctgctt taaaaaatcc tcaagcgcaa aattggtatt
     1741 gaaagttttt tcaaatcgct tgcgcatatc catatctgcg atgtgtctcg ttattgactg
     1801 ttttttttca acgaaatgtt gctcatatgc tgtgagatca tcttttttat tctctgattg
     1861 tgaagtgtgt tgtgtgttat ccatattgtg cacctgtaaa acaatgcgcc gtacttcctc
     1921 acatcacggc gctgatggtt tctgtctgtt tcatagagcc gcagaagaaa cagcgagcta
     1981 tcagcctggc ggtcggtttt tagtggcttt taaccgccgt gggtatttct cgacaaaagc
     2041 ctcaagggca aatcgtgttg gcctggtgag tggctgtgga ttaagtaaca accttctttg
     2101 ccgttgtgct ttttcgcgct gtcgctcaag tatggataat gcgatttcct gtagttcttc
     2161 atttgtcatt gtctgtacct gtaaagcaat acgccgtaac acaccacgct acagcgatga
     2221 tggtttctgt tgttgtaatt tattgcgttg gttttttctg ccactgagaa acacctcaag
     2281 cggtggcggt tcgctggttt tttctggcgg ggttaatttt tctttcctgg tttcaatgat
     2341 gtcacgtatc agtcgctggc gttgtagtgc gctgatattc ggggtaacgg gcgaaaatct
     2401 gtgcttactc atactggatt ccggtaatgc attatgtcgt gatgcctcat atcacggcgc
     2461 tggttattgt ctggtacgtt gtctttcttt ctggtatcca tcctttctgg aaggatatgt
     2521 gaatattttt cgcacaaact tttctcgcaa cacatccgac cacttaagca aaaaagcggc
     2581 atatactgag gctatccctt ataaaacggg gatcgggatt ggcctcccgc tggagttaga
     2641 ggcgatacac gacgcgccag cgtctttttt tgtatcggcg cacacgcaca cctcaacaat
     2701 ggtgggctgt atggaacttc cttcgggaag gctggtttcc tctaacacca gtaaggccaa
     2761 ttccgtacag tctaccgcca gcaagattgg cctcttgggc ggtgggtata atcccaaagt
     2821 cagaggagcc tttcacatgg ctgtatccgc acgcccttac tttgtctggc gctttatgca
     2881 gtgcctgaca gacagcattg caatattcac cgttaccgct gccactgagc gcgaagcacg
     2941 cgcccagctg ccgcacgcac atcttatttt tgtcgcccgt atccgtcagg aggtgactca
     3001 tgcttaaaac cttccgcgtc ttttcccgtg ctgttaacaa gcgtggctat accgttggca
     3061 tcacgcagaa cgtgaaggcc gaaaacgtcc aggctgcaat cagcacagta attaacaaat
     3121 cagctaaagc cggtttatcc ggcgtaatta tccacgcagt gacatgtatt caggatgagg
     3181 agtaattcat gtgtaatttt tccgatatga ctgttgatga agcagcaaaa agcgcaatgc
     3241 aggcagaact tatttgttct ctgatgatgg gtaacacagg tgaaatgact gagggtgaga
     3301 ttgagtcatt gctcgccctg attaagcagc ttacaggtaa ggctggtagc tggctgattg
     3361 cggcatcggg tgatatgcaa taagggaata aataacagga tgaaataaat accagcgaat
     3421 taaagcgtta catatcagga aataaaacga tgaaacgtaa atattctggc ttaactgcca
     3481 gtagcaaaac tcaccccgaa tttatgtgcg gggatactta tcgcaatgaa tacggtagcc
     3541 acatgacgat gatcagcaga ggtaagaaat gaaaagcgca ccgaacttaa aaaaacagcc
     3601 tcgagggaaa aaacacgccg atacggaagt gattattttc gctggcagtg atgcgtgggc
     3661 gcacgcgaaa cagtggctgg agcaggacgg aaaacttgcc ggagataata taccgcccgt
     3721 cgtgctggct gatgagcaac ttaaagatat tggcaatctg caaattgtac ccgacgggcg
     3781 caaatcagcg cgtatataca aggccggaca cctcgatcag gtgatggtca aaggcatagg
     3841 tcagaagctg gcggcggcag gcgtacagga cgcagactat taccccgaag gtatgcacca
     3901 tagcgagcgg caaaactggc gtaactatct ggatattgaa cgcaaaaaca tttctgacgg
     3961 gctggttatt gagttgcctg taaagaaaaa agcgcgtgga cagggtgatg attcgtcact
     4021 ggcacttaac cagatggggg caagccagcg cggagaagtt cttctggcac attatggcgg
     4081 cgaactggca ataaacgccg actctgacac cgtgcattat tacaacagtg ttgtatgggt
     4141 tcccgtacag gataaagagc tacagcgcac aatggcgcaa atcttcattg acgaagatat
     4201 ctgctattca caaaacgcca taaaatcggc ggttgatacc atgaagttaa gtttacccgt
     4261 aatggggaat accgcccgta acctgattgg attcagtaac ggggtatttg atacccgtac
     4321 aggtgatttc cgtgaacacg ataaaaatga ctggctgtta attgccagtg aattaccgtt
     4381 caccccacca gcagagggtg aaacgctggc aacacatgcg ccgaatttct ggaagtggtt
     4441 acgccgttcg gtggctgaga atgaccgcaa ggcagatcgc gttctggcag cgttatttat
     4501 ggtgctggcg aaccggtacg actggcagtt attcattgag gtaacaggtc ctggtggaag
     4561 cggtaaaagt gttatggcag agatttgtac catgctggca ggtaaggcca acacggtatc
     4621 ggcaagcatg aaggcactgg aggatgcaag ggaacgcgca ttagtggttg gcttttcact
     4681 gattattctg ccggatatga cccgctacgc gggtgatggt gcagggataa aggccattac
     4741 gggcggtgac aaggtggcaa ttgacccgaa acacaaagcg ccatactcaa cgcgtattcc
     4801 ggcggttgtg ctggcggtta acaacagcgc catgtcattc agtgaccgca gcggggggat
     4861 ctcacgtcgt cgtgtgatat tcaatttttc ggaggttgta ccggaaaacg aacgcgatcc
     4921 gatgctgccg gaaaaaatag aaggtgagct tgctgtagtg attcgccatt tgcttacgcg
     4981 cttttctgac caggacgaag ccagactcct gttacatgag cagcagaaat cagaggaagc
     5041 ccttgcgata aagcgtgagg gggattcgct ggtggacttc tgcggctatc tcatgtcgtc
     5101 ggtaatgtgt gacggcctgt tagtcggtaa tgctgagatt attcctttca gtccacgccg
     5161 ttatctctat cacgcctatc tggcttatat gagggcgcac gggttcagta agcccgtaac
     5221 actgacgcgc tttggtgcgg atatgccagg tgcaatggcg gaatatggca gggaatatat
     5281 gaaacggaaa acgaagcacg gattgcgctc aaatgtgacg ctgacagagg aatcggagga
     5341 ctggatgccg tcatgtgcac cagtgaaaaa tgagggtgat aaaaattaag ttatagaaaa
     5401 actgttcacc actgttcacc ctgtaataaa tgtatttagt atcaatacgt taaggggtga
     5461 acagttttgt aaaactattc acccaagtgt tcacctgttc accatgttgt ttgatttttg
     5521 aacagcaaag ggtgaacagg gtgaacagtt ggtgaacacc tttacaaaaa gtgttcaccc
     5581 cttaagttag tgaaaataaa ggcaaaacag caaaaggtga acagttgaac accttttggg
     5641 tataaatttt tatttattcc tctgagaaaa attttatgac agagaaacac gccaaaaaat
     5701 cacattcaca cgctatcgat ttaaccgaac cggaatctgt tgtgaggttc gtatcggtac
     5761 gaggcagagc cacgctttat atacctgacg aacatctgca tcactgcgac aaacataaac
     5821 ttccgatcct gattgtctgg aagcgaacgg tatacgccga tgtgacatgg ctgaatgatt
     5881 cgctgatgtt gatacaccgt gacctgtttg aacgtgaaga gttccggcgg gatattgaag
     5941 agcgggcagg aaaaatctat gagaaatatg cagcaaacag caaaagagcc gcacgggcga
     6001 tcacttacca tttcatgacg ctgtatgacc tgaaagcgga tgatgcagaa aaagcggcat
     6061 gtgatttatt cgatatgaca atgggtatta tccaggagta ccggaacaag gagcgcagac
     6121 catgacagca cagattgcag cttacgggcg gctggtggac gacccgcagg taaaacagac
     6181 cagcaaggga acagcgatga cgctggcgcg tatggcggta tctttgccat gcagtcaggc
     6241 acaggacgga caggcgacgt tatggctatc agtcctggca tttggtaagc aggccgacgc
     6301 gctggcgaga catgaaaaag gcgaccttat cagcgtggcg ggtaacatgc agattagcca
     6361 gtggaccgga cagaacggag aaatacggca gggctggcag gttatcgctg acagcgtaat
     6421 cagtgcccgt tcggcgcgtc ctggcgggaa gcgggggcaa caggggaagg cgaccgaagc
     6481 actggaaaga gcaaaacgac agaccggaca gcagggaaat cagccaccag cgggaggtga
     6541 tgacccgttc tctgacgata ttccgttctg aatgacagga gcacaacatg aaccagcaga
     6601 ccacacaaca ggaccagacc acacgtaaac catcacggcg cataccgtgg cagcacatga
     6661 caaaagtgca acgaaaaaac cacagacgaa taaatcgaat tgcggaatac tggcctgagc
     6721 tgtttgggac agagaatata aaacccctga aggctggcat aatacgtgat gtgcttgagg
     6781 acgctaaatc acgggagttg ccacttgcta caggtgtatt acgcggcgca ttaacctcac
     6841 accttcacac gacgcgatac cttaaggcga ttattgctgg tggcccacgg tatgacctga
     6901 acgggcagcc ttgcggcgaa gtcacggaag aagataaatc agtcgccagt gaactactga
     6961 aacggagact ggcgcaaatt aagcagcaga acagccagca gcaaacaacc ggagacgatg
     7021 gtgatgatag ctgacagcgt ggcgcaaaaa cttgaagaac gcggattatg gcgacgagca
     7081 gctacccgat ggggtgacgt ccttcttcac gcagaaacgg acagggaacg cgaggaagct
     7141 gccagacgtc gggcgatatg tatcattaaa tcccgtcgta tgcctgaaca actggtcacc
     7201 tttggcgatg tgaaaaaggc cgctgacaga acgctgaaag agatggggat taatgcgcag
     7261 gatgaatgga aaaattacag cttcagtgat gcaggcgacg atttagcgct accgtgatac
     7321 gtatctcatc accgtatttg ctcagttatc gcgattcgct ataattacca ccgaagagcc
     7381 gttgatggtg ttcgcctctg gcaactggag ccagactgca tgaagccatt cagcggcttt
     7441 tcggcactca ttttgatgtg tacttgtcac acacatcggg atgagtgaac gacgaaaaca
     7501 tcacggtgat ttcgtcatgc cgcgataaga atgcgcctct ttgggcaact ggagccctct
     7561 gtacagcggg ttattgcggc aaagttgtga taaaaacgcg cctctttgag caattgaagc
     7621 tcactgtaaa gcggcttatc acagcaggct gtaacgggcg ctcatcttct ggcaactgaa
     7681 gccacctgta cagagacccg ttacggctga ccctgaagga tttcgccccg tgcaattgaa
     7741 gcacccagcg gagttattca gggtttttta ttgcccgtcc accagcacaa ccagcaccct
     7801 taccagtacg gttaacctgg tcattatcag gcaggccaga agccccttcg gtggggtgaa
     7861 aaatccacgg aaaaatccct gaaaaaatgc acagaaaatt gcgcaaaaaa atgcgcaaat
     7921 cgatcacaaa accgatcaca aaaccgatca cacagaccag cagagcaaag ccagatatga
     7981 cgaggcttgc agggggacag accacctcaa aaagtgcgca aaaaactgcg catttttctc
     8041 aaaaacgaaa cgcaacagcc gcgcaaaacg aaaccgatac gtgatgatcg aaaaagtgcg
     8101 caaatttacc ggattcaggc aaggctgttt accggattcg ggtaagcaat tttgtctgat
     8161 ttgtgcaaaa tatggtgttg ccgtaatagt gacattgtca tcaaaacagg gacacacagg
     8221 agggccgatg aatgagcgtt caccaacaga ggtaattcac gggattattg aagaacagga
     8281 ggagcgcgat ttaatcctca tgagtgaaga gcagacagaa cgccagcgca aacaggaggc
     8341 cacacagccg gacagcgtga cctctgaaaa ttatgcgcgt gttgtggctg atgtgccgtg
     8401 atggtgtcga actgagtagc tttaagcgac tctgtgacta gttttaaaca agtcgcaaaa
     8461 agagccggag aaaaactacg aatctggggt ttttgctaag cactctgtac acggtggaac
     8521 atatggtggt caggagacgg caggagcaac gccagaacgc gcccccgtac cagtggcata
     8581 agccggagcg gttacagcgg taacttgttg attaaaaggc ggctcaattc tgagccgggt
     8641 agctgacggc gttaactggc tgatattgta ccaaagcact ttttaatgct ttggtgctgt
     8701 aacttactga acttaaagca aagcgcaaaa ttgcgctggc tacaatgaaa aacgtaatcg
     8761 cctgataatt atgccacgag acaaagggcg gtgcaccctg aatggtcata atgaccactc
     8821 agaccatcaa ggcaactata acgaacatcg ttacagttgg agcttgtacc ccgttggggg
     8881 aaaaggtgca tacctgaatc atcattatgg tgatcaaggg tatgtaaagc ccaccagcct
     8941 gattaaaggt taaccggaaa aagccaggta tggagggagt ggttagccac gaacaagccg
     9001 gacaccacca gcagaaggta ttatgatttt cgtaatacct ttagcaaagc gtgaatcatc
     9061 attaccgtag ggatggtcat aatgaccaca ccttccccca ctggaacaag gaaggctggc
     9121 ttacgaaaat ttcgtaaccc tccccagcac aatgtattaa ataatcacta caggccattg
     9181 attaaactta attatcgcga atcgcgagaa ttagccccgc cggaacaagg aaagggaaat
     9241 tacgaaaatt tcgtaaaatc cccccacatc tcccacggtt gcccgttaag tgcaagcgca
     9301 caggcacgac ctcattaacc agttaccagc gctcacgctc aaacatccac agcaatgggg
     9361 ctaaatcgca ttctggcgca atgttcactg tcagggcgag cattcaccgg aaaacgtcat
     9421 acaggcttac aggcggctta aaattcgatg ggcggaggac cggatgggaa tcgggagacc
     9481 tggacaaaaa gtgcaggcct ccatgacacg gtcaggacag tttgtgttta accagaatat
     9541 ttagcgcctg tattggggct tcgtggagct cccgcagaca ttcagggtca gtaacatgtt
     9601 cctggtatct tgttttctca agtaacaagc ggtgcatatg cagcatacgg gcaagagccg
     9661 cgcacacctc ctcaaatgcg gtatcccgct taccgtcact gtcagagcca caaaacagcg
     9721 gtacaacctc cacgccgtcg ctgtgggcct ttagccaggc atcaaccacg gttaaatcat
     9781 cggtgaagaa ccacgcactg ttagcgctga atcgcatttt ccaggcttcc ggtactggtt
     9841 gcggggagta tgaatatgct ggtggtatct tgtcgtgagt atccataggc tatcgttccg
     9901 tcattctgtt attcaccaca gcgctaaaaa cgccatgaag gaccagaata ccgattgcct
     9961 cgtttgctgt atgaggtgct taccacacat tgggtacaca gatgaatcag gcggaagtgt
    10021 gattaccgga aaaaacccct ttcagtttga cgaaaaaaaa accgcagtgt gataacacta
    10081 cggcacgcca ccaaagaaac ataacaagga atcagcaatg agtaatacct gtaagccttc
    10141 ggggtgattg taacacatga gcaatctttt actggaagct gtttttcact ggatcacgta
    10201 tcgttatggg gattcccata tcggagagca tccaccagct tcatagcgga taaccggaaa
    10261 aattccggta aatatttgcc tgagtaagtc atcaagcgcc tgctaaatgt gtatagaaat
    10321 gtgtatagga ttgattttag aatatgtggg aatggcttta ttgctggtgg catggcttgt
    10381 tgttctgttc c
//
LOCUS       DAZUCC010000177        10331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZUCC010000177 DAZUCC010000000
VERSION     DAZUCC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.178"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..318
                     /gene="adiY"
                     /locus_tag="ACHY09_003884"
     CDS             <1..318
                     /gene="adiY"
                     /locus_tag="ACHY09_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFS3886792.1"
                     /translation="DSVYQIIESDIHKDWNLSMVASCLCLSPSLLKKKLKSENTSYSQ
                     IITTCRMRYAVNELMMDGKNISQVSQSCGYNSTSYFISVFKDFYGMTPLHYVSQHRER
                     TVA"
     gene            455..1792
                     /gene="adiC"
                     /locus_tag="ACHY09_003885"
     CDS             455..1792
                     /gene="adiC"
                     /locus_tag="ACHY09_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFS3886793.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            1896..3539
                     /gene="eptA"
                     /locus_tag="ACHY09_003886"
     CDS             1896..3539
                     /gene="eptA"
                     /locus_tag="ACHY09_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFS3886794.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKITPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            3536..4204
                     /gene="basR"
                     /locus_tag="ACHY09_003887"
     CDS             3536..4204
                     /gene="basR"
                     /locus_tag="ACHY09_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFS3886795.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4205..5305
                     /gene="pmrB"
                     /locus_tag="ACHY09_003888"
     CDS             4205..5305
                     /gene="pmrB"
                     /locus_tag="ACHY09_003888"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFS3886796.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQNLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5281..5370)
                     /gene="pmrR"
                     /locus_tag="ACHY09_003889"
     CDS             complement(5281..5370)
                     /gene="pmrR"
                     /locus_tag="ACHY09_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFS3886797.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5482..6984)
                     /gene="proP"
                     /locus_tag="ACHY09_003890"
     CDS             complement(5482..6984)
                     /gene="proP"
                     /locus_tag="ACHY09_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFS3886798.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7248..8126)
                     /locus_tag="ACHY09_003891"
     CDS             complement(7248..8126)
                     /locus_tag="ACHY09_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFS3886799.1"
                     /translation="MTKTLLDGPGRVLESIHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDAFRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8123..10331)
                     /gene="crfC"
                     /locus_tag="ACHY09_003892"
                     /pseudo
     CDS             complement(8123..10331)
                     /gene="crfC"
                     /locus_tag="ACHY09_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
BASE COUNT         2578 a         2733 c         2560 g         2460 t
ORIGIN      
        1 gacagcgttt atcaaattat tgaaagcgat atccacaaag actggaatct tagtatggta
       61 gccagttgtt tatgtcttag cccaagtctg ttaaagaaaa agctgaaaag cgaaaacacc
      121 agttatagcc aaataatcac cacctgccgc atgcgctatg ccgtaaatga attaatgatg
      181 gacggtaaaa atatctctca ggtatcacag tcctgcggct acaacagtac gtcgtacttt
      241 atttctgtct ttaaagactt ctacggtatg acgccgctgc attatgttag tcagcacaga
      301 gaacgcactg tcgcctgatt tttaacctta acgaagagct atattaataa cggcatcagc
      361 gataacccgg ccgataataa ttcaactatc gaatgcaggc gtatgatacg acgtaattta
      421 ttgtcacgaa gctcgccttc gcaggagttt aattatgtct tcggatgctg atgctcacaa
      481 agtgggctta atccccgtca ccctgatggt gtcggggaat attatggggt caggtgtttt
      541 tctgttacct gcaaacctgg cctccactgg cgggattgcc atttatggat ggttggtgac
      601 gattatcggt gcgctggggc tctcgatggt atacgccaaa atgtcgttcc tcgacccaag
      661 tcctggtggt tcttacgctt acgcccgccg ctgctttggc ccgtttctcg gttatcaaac
      721 caacgtcctc tactggctgg cctgctggat cggcaatatc gccatggtgg tcattggcgt
      781 aggatattta agttacttct tcccgattct gaaagaccca ttggtattaa ccatcacctg
      841 cgtcgtggtg ctgtggatct tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg
      901 cgtacaggcc gttgctaccg tgctggcgct gatccccatc gtcgggattg ccgtatttgg
      961 ctggttctgg ttccgtggtg aaacctatat ggcggcatgg aacgtcagcg gtctgggcac
     1021 cttcggtgcg attcaaagta cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag
     1081 tgcctccgtt gccgcaggtg tggtgaaaaa cccgaaacgc aacgtcccta tcgccaccat
     1141 tggcggggta ttgattgctg ccgtttgcta tgtactttct accaccgcga ttatggggat
     1201 gatccctaat gccgcactgc gcgtttctgc ttcgccattc ggtgatgccg cacggatggc
     1261 gttgggtgac accgccgggg ccattgtttc cttctgcgca gctgcgggtt gcttaggttc
     1321 actgggcggc tggacgttgc tggcgggtca aacggcgaaa gccgctgccg atgacggact
     1381 gttcccaccg atttttgccc gtgttaataa agcgggcacg ccagtggcgg ggctgattat
     1441 cgtcggtatt ttgatgacca tcttccagct cagtagcatt tcgccaaacg cgaccaaaga
     1501 attcggtctg gtttcttccg tgtcggtcat ctttacactg gtgccatatc tttacacctg
     1561 tgcggcgtta ctactgctcg gccacggtca ctttggtaaa gcacgcccgg catacctggc
     1621 agtgactacc attgccttcc tctactgcat ctgggccgtg gtagggtccg gcgcgaaaga
     1681 ggttatgtgg tcatttgtca ctctgatggt aatcaccgcc atgtatgcgc tgaattacaa
     1741 ccggctgcat aaaaacccgt atcccttaga tgcaccaata agcaaagatt aattcccctt
     1801 aatcaagcaa acataaaagc caaccttaag aacttaaggt tggcttaatt ttgctttgcg
     1861 agcatatgcg cactttgttc gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac
     1921 cctctttaaa tttattctcc tggttattac ttgctacttt ttatatctca gtctgcctga
     1981 atatcgcctt ttttaaacag gtgctacagg tgttaccgct ggattcactg cataacgtgc
     2041 tggttttctt gtcgatgccg gtcgtcgcat tcagcgtgat taatattgtc ctgacactag
     2101 gctctttctt atggcttaat cgtccactcg cctgcctgtt tattctggtc ggtgcggctg
     2161 cacaatattt catcatgacc tatggcatcg tcatcgaccg ttcaatgatc accaatatca
     2221 ttgatactac gccggcggaa agttatgccc tgatgacgcc acgaatgtta ttaacgctgg
     2281 ggttaagtgg cgtgttagcc gcgattattg cctgctggat caaaatcacc cccaccacct
     2341 cacgcctgcg cagcgttctt ttccgcgggg ccaacatcct gatttctgta ctgctgattc
     2401 tgctggttgc cgcactgttc tacaaagact atgcctcgct gtttcgcaac aacaaagagc
     2461 tggtgaaatc cttaagcccg tccaacagca ttgttgctag ctggtcgtgg tactctcacc
     2521 agcggtcagc caacctgcca ctgatacgca ttggtgaaga tgctcaccgc aacccattaa
     2581 tgcaaaatgg gaaacgtaaa aacctgacca ttttgatcgt cggagaaacg tcgcgcgcag
     2641 agaatttctc gctgaatggt tatccacgcg aaaccaatcc tcgtctggca aaagataacg
     2701 tggtctattt ccctaatacc gcctcttgcg gcacggcaac ggcagtttca gtaccatgca
     2761 tgttctcgga tatgccgcgt gagcactaca aagaagagct ggcacagcac caggaaggcg
     2821 tgctggatat cattcagcga gcgggcatca acgtgctgtg gaatgacaac gatggcggct
     2881 gtaaaggcgt ttgcgatcgc gtacctcacc agaacgtcac cgcgctgaat ctgccaggtc
     2941 agtgcatcaa cggcgaatgc tatgacgaag tgctgttcca cgggctggaa gagtacatca
     3001 ataacctgca gggtgatggc gtgattgtct tacacaccat cggcagccac ggtccgacct
     3061 attacaaccg ctatccgccg cagttcagga aatttacccc aacctgtgac actaacgaga
     3121 tccagacctg taccaaagag caactggtga acacttacga caacacgctg gtttacgtcg
     3181 actatattgt tgataaagcg attaatctgc tgaaagaaca tcaggataaa tttaccacca
     3241 gcctggttta tctttctgac cacggtgagt cgttaggtga aaatggcatc tatctgcacg
     3301 gtctgcctta tgccatcgcc ccggatagcc aaaaacaggt gccgatgctg ctgtggctgt
     3361 cggaggatta tcaaaaacgg tatcaggttg accagaactg cctgcaaaaa caggcgcaaa
     3421 cgcaacacta ttcacaagat aatttattct ccacgctatt gggattaact ggcgttgaga
     3481 cgaagtatta ccaggctgcg gatgatattc tgcaaacttg caggagagtg agtgaatgaa
     3541 aattctgatt gttgaagacg atacgctgtt attgcaggga ttaattctgg cggcgcaaac
     3601 cgaaggctac gcgtgcgatg gcgtgacaac cgcgcggatg gcggaacaaa gccttgaggc
     3661 cggtcattac agcctggtgg tactggattt agggttaccc gacgaagatg gactgcattt
     3721 tctcgcccgt atccggcaga aaaaatacac cctgccggta ctgatcctca ccgctcgcga
     3781 tacgctgacc gacaaaatcg ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc
     3841 ttttgcgctg gaagagttac acgcccgtat ccgcgccctg ctacgacgcc ataataatca
     3901 gggcgaaagt gagctgattg ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg
     3961 gatgggcggt gaagagttga ttctgacgcc caaagaatat gctctgctgt cacggttaat
     4021 gctcaaagca ggcagtccgg tgcatcggga aattctctac aacgacatct ataactggga
     4081 caatgaaccc tcgaccaaca ccctggaagt gcatatccac aatctgcgcg acaaagtggg
     4141 caaagcccgt atccgcaccg tgcgcggctt tggctatatg ctggtcgcaa atgaggaaaa
     4201 ctaattgaat ctgatgcgtt ttctgcgccg accaatatcg ctgcgccaac ggctgatatt
     4261 gaccattggg gccattttgt tggtgtttga gctgatcagc gtcttctggc tatggcatga
     4321 aagtaccgag cagattcagc tgtttgagca ggcgctacgc gacaatcgca acaacgatcg
     4381 ccacatcatg cgtgagatcc gcgaggcggt cgccagcctg atcgtccccg gcgtctttat
     4441 ggtcagcctg acgctattta tctgctatca ggcggtacgc cgcatcaccc ggccgctggc
     4501 ggagctgcaa aaagagctgg aagcgcgcac cgccgacaac ttaacgccta ttgccattca
     4561 cagcgccacc ctcgaaatcg aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct
     4621 gaccagcacg ctggataacg aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac
     4681 gccactggcg ggggtgcgtt tgcatctgga actgctggcg aaaacgcatc acattgatgt
     4741 agcaccgtta gtggctcgac ttgatcagat gatggagagt gtctcccagc tgctgcaact
     4801 ggcgcgtgcc ggacagtcat tttcttccgg taattatcaa catgtaaaac tgctggaaga
     4861 tgtcattctc ccctcgtatg acgaactcag taccatgctc gaccagcgac agcaaaacct
     4921 gctattgcca gagagcgccg ccgacatcac cgttcagggt gatgcaaccc tgctgcggat
     4981 gttattgcga aacctggtag aaaacgccca tcgttacagc ccgcaaggca gcaacattat
     5041 gattaagctg caagaagatg gcggagcggt catggccgtt gaagatgaag gaccgggtat
     5101 tgatgagagt aaatgcgggg agttgagtaa agcgtttgta cgtatggaca gccgttatgg
     5161 cgggattggt ctggggttaa gtattgtcag ccgcattaca cagttgcatc acgggcagtt
     5221 tttcctgcaa aaccggcaag agacttccgg cacgcgggcc tgggtacggc tgaagaaaga
     5281 tcagaacgtg gcaaaccaga tataaagaaa gctgctgacc accagcacgc tgaacacggt
     5341 agttaaactt tcataaacac ggtttttcat tgcactctcc tccgttaacc tggaggagag
     5401 tatgcgcgtc agagattaag tgaaccttaa gagttcaatt ggcctgataa gacagcgtca
     5461 catcaggcca tccgtttcag cttattcatc aattcgcgga tgttgctgca ccaggcgagt
     5521 acgtttcgcc tgcaaatcgg caatctcgtg gtcaatatca tcgattttct gctcgatatt
     5581 atcgtaatgc tcgacgagaa tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc
     5641 acctttcaac ggacgatttg ccgtctcttt catggttacg ccggtgatta aaccaaccac
     5701 cgccactacc atcaggtaat aggcaggcat catcagattc tgcgagcttt cgaccagcca
     5761 ggccgccagt gttggcgtca gaccggcaac cagcaccgaa atattaaatg ccgccgccag
     5821 cgcgctgtaa cggatatgcg tcggaaacat cgctggcaag gtagaagcca taacgcccgt
     5881 aaagcagtta aggatcaccg ccagcatcag taacccggca aaaatcaggc cgatgacgtt
     5941 actgttaatc agaataaacg ccgggatcgc caacacaaac agtgcaacac taccaagtag
     6001 cacaaacgga cgacggccaa aacggtcact cagcaagccc atcaccggct ggacaaacag
     6061 cataccgatc ataatggcga taataatcag caccccgtgg tcttcggaat aatgcaggtt
     6121 atgcgacaaa taactcggca tataggtcag caacatgtag taagtcacgt tggtggcaat
     6181 taccagacca atacatgtca acaggctgcg ccagtgtttg gtggcaatct ctttaaacga
     6241 gactttcggg ccatcctgca aaccttcgcg gtcgccctgc tccagtttat caacatgctg
     6301 ctggaacgcc ggggtctctt ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg
     6361 cagagcaata aagaacggaa tacgccagcc ccagtcgagg aagttcgctt cgccgacaat
     6421 ggtcgaaatt aacaccacca cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc
     6481 cagccagctg cccataaagc cacgtttacg gtcaggggag tattccgcaa caaatatcga
     6541 cgccccggta tattcaccgc cgaccgagaa accttgtgcc atcttacaga tcaacagcag
     6601 aatcggtgcc caaataccaa tcgtgtcgta ggacggtatt aagccaatac agaacgtact
     6661 gatcgacata atcacaatag tgatagcgag gatcttctgg cgaccatatt tatcgcccaa
     6721 cataccaaag aagagtccac caagcggtcg aatcagaaag ggaacggaga aagtggcaag
     6781 tgcagcaacc atctgcacgc tggggtcagc ccccgggaaa aaaactttac ctaatgcgta
     6841 agcaacaaaa ccataaacac caaaatcaaa ccattccatt gcattaccca gtgatgctgc
     6901 ggtaatggct ttacgcagtt taccgtcatc aataatggtg acatcacgaa gggtaattgg
     6961 ttttactttt ttccttttca gcatagcttt cctcgcagag ttggcccatt tccgcaaacg
     7021 ccagaaaaat cagcgcgacg gccgcaaaag gggagcggga attgcaggat gcaatctctg
     7081 taacaagcgt agcgggtttg cttacactct ccggttaagg aatgtacaat caaaccagtt
     7141 gacgcctgaa ttgggcagtt aatgaccttt ttaccctacc agcgtttaaa tttgtgatca
     7201 acttcacaca tggatttcgt ttaggactca ttgatgtaac tgattattta caccaaattt
     7261 acagagaaga tttcctcgac cattgccccc gccagacgct gcgctgaaca gagtcgtggc
     7321 atccccagtg cgacagttcg ccagcgttgg gtcactgacc agcaaacggg ttgtttatct
     7381 gccgtggacc actttcccag ccaacttaaa aaatcagcat gatccccgcc cgtcggaatg
     7441 gtcgaagagg caaaccactg atggtaatag tgacgcgtgg tcggggccgc attgatactc
     7501 tctgccagat gttgtgtcgc tttcacgcgc aaatcaccca gcaacaacgc caccgcttgc
     7561 gggcttccca gacgaaaggc atcgccaaat gccccccaac gcaactcctc cagaacgata
     7621 aaacagcgcc caggcaacga ccaggcgttg tacgccccag cacgccagcg ggtaaaaatt
     7681 tgctcgctct gttcacccgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc
     7741 cagcgacaac gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctcaggg
     7801 aaagcagagt gctggcggtg atccattttt tgcagcagcg ccagatgttg cgcgatctgc
     7861 gtcagcgcca gatgaccggg atccagcatc gacgccagtt tttccaccaa tgtcagacgc
     7921 aggctaagcg gcgcatttaa catcccgccg ttcgtccatg cctgaagctg cacacgcgca
     7981 agaagttcct gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg atggcgggca
     8041 tcatcgccct gcgccagatc cactaaaaag cggggatgaa tcgactccag cacgcgaccg
     8101 gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat
     8161 cgcgtaacag ctgagcgtcg gtgtaaatcc agccatgagt catcacagtc tgctgcaatt
     8221 gctgctgcat cagcctgacc accgattcat tttgttgacg cagagccaga ctttcgcgta
     8281 aacgcgtctg taattccgtc aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg
     8341 ccgtaacaga ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat
     8401 cgacttctgc gcttaatgct cgttgcaacg cactgtaatc aaccgtctct atcggggatt
     8461 tctcatttcc ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa
     8521 cgcctaaacg cgtgctctgc tcgtccattg cgtcctggcg cgaaatgact tcactgaaca
     8581 gctggcgagt gttgaagtgg ggaactacgc cgtgaaaaac aggaaaatga aacccaggac
     8641 gaaaccctga ctcgctcaat tccattttga tttgttgctc aatggggcga ataacatcgg
     8701 ttaacacccg ggcaaagggt ggattccagc tcggcaaaac gcagcgtaaa gtcgcgacta
     8761 atggtgttct gcgccatctg taacagtgtc tcacagcagg tacgaatctc gcttaatgac
     8821 cctgaatcat cctgaaacaa ggcggctaac tgcgcattca gcgcatcttg ctgttgacgc
     8881 agaaagtggt tggcggaggt cagggccagc tcgatttcat gtttaatctc gccgctcacc
     8941 tgcgcctgat tgagttgcaa tagctgcaaa ctttcttcga tctgatggat attttgccgc
     9001 aattgttcac aagcgacgtt taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc
     9061 tgcgcgtaat tcaacagttt atgcgcggca gatcgcagag catacaacga ggcgttagcg
     9121 taagcggcat gaagcaacgc ctgaattggc tgggcgaaca gcgaatcttc ccacaactga
     9181 tcggcagcat gacgaatatg ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg
     9241 agcgcggcat gggcaaaatc ttccacccag cgttgttgct ctggcggtgg taacttaccg
     9301 ttgttggcta actcatggcg cacccgattc gccaggtagc cccacatcga cgacaccgga
     9361 aatatctgct gtggcgtaat acaacctttc atcagcgtcc cggaaatcag tgcccgcacc
     9421 tggtcggcgt cgtcactgtt acgatcctgt tgatcgaact tattgaccag cacatacaac
     9481 ggcaccgatt gccccaccgc caaaattgcc tcacggacct cttcatcgga gatcgatttc
     9541 agttgcgtat aatccagcac cgccagtacc gccgaggcgc gtgccagctg ctggttaagc
     9601 attttttgca gatgcggttg cccggcttca tttggcccgg gggtatccag taacgtcaac
     9661 tgaccgggat aactctccag ccccgccaga tggacaaact ccacttcaat cacgggaata
     9721 tgctcaatgg cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc
     9781 accaaatcat tcaaactttt cagacaatga aaaataggtt gagcacccag ataatatttt
     9841 tcgaaagcga tgccattttc aatccgctgc ataagcgcac gcatatcttt atctatttcc
     9901 agcacatcgg tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa ttgttgaatt
     9961 aaatggtcga ttggcgcgac atgtgaaaaa tgcagtaccg gttctttttg cccgggcgta
    10021 tggcgaataa gcgttggcag cgcagtcatt gggcgattac gattaggcag aacctccgta
    10081 ccaacaatgg cattaatggt ggttgatttc cctgctttca tggtaccgac aatcgccagc
    10141 accatttcca gccgggaaat tttacgcaac tcattattca gcatcgcgtg acgttcggcg
    10201 atattaggct gactccaggg taaagccagt tgtggcgtat cgtctccggg tacagagaga
    10261 ggtatttttt ccagtaactg caactgttgg cgagaaagct gtaacaggcg ttcagcctcc
    10321 tgacttaact c
//
LOCUS       DAZUCC010000178        10315 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZUCC010000178 DAZUCC010000000
VERSION     DAZUCC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.179"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            200..355
                     /gene="rseD"
                     /locus_tag="ACHY09_003893"
     CDS             200..355
                     /gene="rseD"
                     /locus_tag="ACHY09_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFS3886800.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            352..927
                     /gene="rpoE"
                     /locus_tag="ACHY09_003894"
     CDS             352..927
                     /gene="rpoE"
                     /locus_tag="ACHY09_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFS3886801.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            960..1610
                     /gene="rseA"
                     /locus_tag="ACHY09_003895"
     CDS             960..1610
                     /gene="rseA"
                     /locus_tag="ACHY09_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFS3886802.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            1610..2566
                     /gene="rseB"
                     /locus_tag="ACHY09_003896"
     CDS             1610..2566
                     /gene="rseB"
                     /locus_tag="ACHY09_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFS3886803.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            2563..3042
                     /gene="rseC"
                     /locus_tag="ACHY09_003897"
     CDS             2563..3042
                     /gene="rseC"
                     /locus_tag="ACHY09_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFS3886804.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            3240..5039
                     /gene="lepA"
                     /locus_tag="ACHY09_003898"
     CDS             3240..5039
                     /gene="lepA"
                     /locus_tag="ACHY09_003898"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS3886805.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5055..6029
                     /gene="lepB"
                     /locus_tag="ACHY09_003899"
     CDS             5055..6029
                     /gene="lepB"
                     /locus_tag="ACHY09_003899"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS3886806.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            6301..6981
                     /gene="rnc"
                     /locus_tag="ACHY09_003900"
     CDS             6301..6981
                     /gene="rnc"
                     /locus_tag="ACHY09_003900"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS3886807.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            6978..7883
                     /gene="era"
                     /locus_tag="ACHY09_003901"
     CDS             6978..7883
                     /gene="era"
                     /locus_tag="ACHY09_003901"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS3886808.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            7895..8623
                     /gene="recO"
                     /locus_tag="ACHY09_003902"
     CDS             7895..8623
                     /gene="recO"
                     /locus_tag="ACHY09_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS3886809.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGESVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            8635..9366
                     /gene="pdxJ"
                     /locus_tag="ACHY09_003903"
     CDS             8635..9366
                     /gene="pdxJ"
                     /locus_tag="ACHY09_003903"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFS3886810.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIPVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            9366..9746
                     /gene="acpS"
                     /locus_tag="ACHY09_003904"
     CDS             9366..9746
                     /gene="acpS"
                     /locus_tag="ACHY09_003904"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS3886811.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10168..10248
                     /gene="shoB"
                     /locus_tag="ACHY09_003905"
     CDS             10168..10248
                     /gene="shoB"
                     /locus_tag="ACHY09_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFS3886812.1"
                     /translation="MTDCRYLIKRIIKIIIAVLQLILLFL"
BASE COUNT         2596 a         2496 c         2766 g         2457 t
ORIGIN      
        1 ttaaacacct gctattttaa tatttgttac agtgactaaa cacgctgact cagggcggcg
       61 agaaaaagag aagttactgg ctggtggagg attaggtggt gaaataaaaa ggccgttggg
      121 ttactcttca ggcagttaaa tgggcatttc tacacagata atgcgatgtt cagattctgt
      181 agacttataa tgatagataa tgatccgtct acagcatgac aaacaaaaac agatgcgtta
      241 cggaacttta caaaaacgag acactctaac cctttgcttg ctcaaattgc agctaatgga
      301 gtggcgtttc gatagcgcgt ggaaatttgg tttggggaga ctttacctcg gatgagcgag
      361 cagttaacgg accaggtcct ggttgaacgg gtccagaagg gagatcagaa agcctttaac
      421 ttactggtag tgcgctatca gcataaagtg gcgagtctgg tttcccgcta tgtgccgtcg
      481 ggtgatgttc ccgatgtggt acaagaagct tttattaaag cctatcgtgc gctggattcg
      541 ttccggggag atagcgcttt ttatacatgg ctgtatcgga ttgctgtaaa tacagcgaaa
      601 aattacctgg ttgctcaggg gcgtcgtcca ccttccagtg atgtggatgc cattgaagct
      661 gaaaacttcg aaagtggcgg cgcgttgaaa gaaatttcga accctgagaa cttaatgttg
      721 tcagaagaac tgagacagat agttttccga actattgagt ccctcccgga agatttacgc
      781 atggcaataa ccttgcggga gctggatggc ctgagctatg aagagatagc cgctatcatg
      841 gattgtccgg taggtacggt gcgttcacgt atcttccgag cgagggaagc tattgataac
      901 aaagttcaac cgcttatcag gcgttgacga tagcgggata ctggataagg gtattaggca
      961 tgcagaaaga acaactttcc gctttaatgg atggcgaaac gctggatagt gagctgctta
     1021 acgaactggc tcataaccca gaaatgcaga aaacctggga aagctatcac ttaatccgtg
     1081 actcaatgcg gggtgatact cccgaggtgc tccatttcga tatctcttca cgcgtgatgg
     1141 ccgccattga agaagagcca gtacgtcaac cggcgacatt gatcccggaa gcccagcctg
     1201 cgccgcatca atggcagaaa atgccattct ggcagaaagt acgtccgtgg gcggcacagc
     1261 ttacccaaat gggcgtagcc gcatgcgtat cgcttgcagt tatcgttggc gtccagcact
     1321 ataatggaca atctgaaacg tcccagcagc ccgaaacgcc ggtatttaat acactgccga
     1381 tgatgggtaa agccagcccg gtaagcctgg gagtaccttc tgaagcgacc gcaaacaatg
     1441 gtcaacagca gcaggtacag gagcagcgtc gtcgcattaa tgcaatgttg caggattacg
     1501 aactgcaacg ccgactccac tctgaacagc ttcagtttga gcaggcgcaa actcagcaag
     1561 ccgctgtaca ggtgccagga attcaaactt taggaacgca atcgcagtaa tgaagcaact
     1621 ttggtttgcc atgtcattag tgacaggtag cctgttattc tctgctaacg cctcggccac
     1681 tcccgcgtcc ggggcgttat tacagcagat gaacctggcc agtcagtcac tgaattacga
     1741 gctgtcattc atcagcatca ataaacaggg tgttgagtct ctgcgttatc gacatgcacg
     1801 cctcgataac cgtcctcttg cacaattgtt gcaaatggat ggcccgcgcc gggaagtggt
     1861 acagcgcggc aatgaaatca gctattttga accgggactt gaaccgttca cgcttaatgg
     1921 cgattacatt gttgattctc taccatcgct catctatacc gatttcaaac gcctttctcc
     1981 ttactacgac tttatctccg tcgggcgcac gcgtattgct gatcgtcttt gcgaagtcat
     2041 tcgtgtggtt gcccgagatg gtacacgcta cagctacatc gtgtggatgg acaccgaatc
     2101 gaaattaccg atgcgggttg atctccttga tcgcgatggt gaaacgctgg aacaatttcg
     2161 cgtgattgct tttaatgtca atcaggatat cagcagcagt atgcagacgc tggcgaaggc
     2221 aaatttgccg ccgttgcttt ctgttcctgt aggtgaaaaa gctaaattca gctggacgcc
     2281 aacctggttg ccacagggtt ttagcgaagt ttccagtagt cgacgtccgc taccgacgat
     2341 ggacaacatg cctatcgaat cacgtctcta ttccgacgga ttattcagct tctcggtaaa
     2401 cgttaaccgc gctacgccat cgagcaccga tcagatgttg cgcaccgggc gcagaaccgt
     2461 cagtacaagc gtacgtgata acgccgaaat caccattgtc ggtgaactgc cgccgcaaac
     2521 ggcgaaacgc attgccgaga atattaagtt cggggcagcg caatgatcaa agagtgggct
     2581 accgtcgtct cctgggaaaa cgggcaggcg ctggttagtt gtgatgttaa agcctcatgc
     2641 agcagctgcg cttcacgtgc cggttgcggt agccgcgtgt tgaataaact tggcccgcaa
     2701 accacgcata ccattgtcgt accctgtgat gagccgttag tgccggggca aaaagtagaa
     2761 ttaggaatcg ccgaaggcag cctgcttagc tccgcattac tggtttatat gtcgccgttg
     2821 gtgggactat tcctcatcgc ttcactattt cagctactct ttgcttcaga tattgcagca
     2881 ttatgcggtg cgattctcgg tggcgttggt ggattcctga ttgcacgtgg ctgctctcga
     2941 aagtttgctg cccggtctga atggcaaccg atcatcttaa gcgtggcctt gccgcctggc
     3001 cttgtgcgat ttgaaacatc ttcggaagac gcgagccagt gatttcactg gctttgttgc
     3061 attttgcata acaaatgcga ggacgtttcc agataccaca atccatacgg tatgaacatt
     3121 tcctcgcctc aatgttgtag tgtagaatgc ggcgtttcta ttaatacaga cgttaagctc
     3181 agaacagcga cttctaaaag cctggttaac cgggcattaa ggcacaataa tcatacttta
     3241 tgaagaatat acgtaacttt tcgatcatag ctcacattga ccacggtaaa tcgacgctgt
     3301 ctgaccgtat tatccagatc tgcggtggcc tgtctgaccg tgaaatggag gcgcaggttc
     3361 tcgattccat ggatcttgag cgtgagcgtg gcattaccat caaagcgcaa agcgtgacgc
     3421 tggactacaa agcgtctgac ggcgaaacct atcagcttaa ctttatcgac accccgggcc
     3481 acgtagactt ctcctatgaa gtttctcgtt cgctggctgc ctgtgaaggt gcattgctgg
     3541 tggtcgacgc cgggcagggc gtagaagcgc aaaccctggc aaactgctac accgccatgg
     3601 aaatggatct cgaagtggtg ccggtactga acaagattga cctgccggca gccgatcctg
     3661 aacgcgtggc ggaagaaatt gaagatatcg tcggcatcga cgccaccgac gcggtgcgct
     3721 gttcagcgaa aaccggcgtt ggcgtacagg acgttctcga acgtctggtg cgcgacattc
     3781 cgccgccgga aggcgatccg gaaggcccgt tgcaggcact cattatcgac tcctggttcg
     3841 acaactacct gggcgttgtt tcacttatcc gtattaaaaa cggcaccctg cgtaagggcg
     3901 acaaagtgaa agtcatgagt accgggcaga cctataacgc cgaccgtctg ggcatcttca
     3961 cgccgaagca ggttgaccgt actgaactga aatgcggcga agtaggctgg ctggtatgtg
     4021 cgattaaaga tatccacggc gctccagtag gcgatacctt aacgctggcg cgtaatccgg
     4081 cagaaaaggc gctgcctggc tttaagaaag tcaaaccgca ggtatacgcc ggtctgttcc
     4141 cggtaagttc cgacgactat gaagccttcc gtgacgcgct gggcaaactc agcctgaacg
     4201 atgcttcact gttctatgag ccggaaagct ccagcgcgct gggctttggt ttccgctgcg
     4261 gcttcctcgg cctgctgcac atggagatca tccaggaacg tctggaacgt gaatacgatc
     4321 tggatctgat caccactgcg ccgaccgtag tgtatgaagt tgaaaccacg tcaagagaag
     4381 ttatctacgt cgacagccca tccaagctgc cagcggtaaa taacatttac gaactgcgcg
     4441 agccgattgc agagtgtcac atgctgctgc cgcaggcata tctcggcaac gttattacgc
     4501 tgtgcgtaga aaaacgcggc gtgcagacca atatggttta ccacggtaat caggtggcgc
     4561 tgacgtacga gatcccgatg gcggaagtgg tgctcgactt cttcgatcgc ctgaaatcta
     4621 cctcgcgtgg ttatgcgtct ctggattaca acttcaaacg cttccaggcg tccgacatgg
     4681 tacgtgtaga cgtattaatc aacggtgaac gtgttgatgc gctggcgttg atcacccacc
     4741 gtgataattc gcaaaaccgc ggtcgcgagc tggtggagaa gatgaaagat ctgatcccac
     4801 gccagcagtt tgatatcgcc attcaggcag cgattggtac gcacatcatt gcgcgctcca
     4861 ccgtgaaaca gctgcgtaaa aacgtactgg ctaaatgtta tggcggcgat atcagccgta
     4921 agaaaaagct gctgcagaag cagaaagaag gtaagaaacg catgaagcag atcggtaacg
     4981 tcgagctgcc gcaggaagcg ttcctcgcca ttctgcacgt cggcaaagac aacaaataac
     5041 ccttaggagt tggcatggcg aatatgtttg ccctgattct ggtgattgcc acactggtga
     5101 cgggcatttt atggtgcgtg gataaattct ttttcgcacc taaacggcgg gaacgtcagg
     5161 cagcggcgca ggcggctgcc ggtgactcac tggataaagc aacgctgaag aaagttgcgc
     5221 cgaagcctgg ctggctggaa accggtgctt ctgtttttcc ggtgctggct atcgtattga
     5281 ttgtgcgttc gtttatttat gaaccgttcc agatcccgtc aggttcgatg atgccgactc
     5341 tgttaatcgg tgattttatt ctggtagaga agtttgctta tggcattaaa gatcctatct
     5401 accagaaaac gctgatcgaa accggtcatc cgaaacgcgg cgatatcgtg gtctttaaat
     5461 atccggaaga tccaaagctt gattacatca agcgcgcggt gggtttgccg ggcgataaag
     5521 tcacttatga tccggtctca aaagagctga cgattcaacc gggatgcagt tccggccagg
     5581 cgtgcgaaaa cgcgctaccg gtcacttact ccaacgtgga accgagcgat ttcgttcaga
     5641 ccttctcacg ccgtaatggt ggggaagcga ccagcggatt ctttgaagtg ccgaaaaacg
     5701 aaaccaaaga aaatggaatt cgtctttccg agcgtaaaga gacattgggt gatgtgacgc
     5761 accgcattct gacagtgccg attgcgcagg accaggtggg gatgtattac cagcagccag
     5821 ggcaacaact ggcaacctgg attgttccgc cgggacaata cttcatgatg ggcgacaacc
     5881 gcgacaacag cgcggacagc cgttactggg gctttgtgcc tgaagcgaat ctggtcggtc
     5941 gggcaacggc tatctggatg agcttcgata agcaagaagg cgaatggccg actggtgtgc
     6001 gcttaagtcg cattggcggt atccattaat agccatcttc gttcacgttg tcgccgttat
     6061 ggcgacaacg tgaattattt atgagataaa tctcccgtgg ctaacgacat cccccgtcgt
     6121 tgtgtataga atattccccc gaagtttaag gttggcacct ccaggttgcc acggcacacg
     6181 aaacagcgtt ggtccccata taccggtaaa ctgaaactgc agtgaagcag ttagccgagc
     6241 catgtatatc aggtctgttt cgtgtgctga attgttgacg catttattta ttggtatcgc
     6301 atgaacccca tcgtaattaa tcggcttcaa cggaagctgg gctacacttt taatcatcag
     6361 gaactgttgc agcaggcatt aactcatcgt agtgccagca gtaaacataa cgagcgttta
     6421 gaatttttag gtgactctat tctgagctac gttatcgcca atgcgcttta tcaccgtttc
     6481 cctcgtgtgg atgaaggcga tatgagccgg atgcgcgcca cgctggtccg tggtaatacg
     6541 ctggctgaac tggcgcgcga atttgagtta ggtgagtgct tacgtttagg gccaggtgaa
     6601 cttaaaagcg gtggatttcg tcgtgagtca attctcgctg acaccgtcga ggcattaatt
     6661 ggtggcgtat tcctcgacag tgatattcaa accgtcgaga aattaatcct caactggtat
     6721 caaactcgtc tggacgaaat tagcccaggc gataaacaaa aagatccgaa aacgcgtctg
     6781 caggaatatt tgcagggtcg ccatctgccg ctgccaactt atctggtagt ccaggtacgt
     6841 ggcgaagcgc acgatcagga atttactatc cactgccagg tcagcggcct gagtgaaccg
     6901 gtggttggca caggttcaag ccgtcgtaag gctgagcagg ctgccgccga acaggcgttg
     6961 aaaaaactgg agctggaatg agcatcgata aaagttactg cggatttatt gctatcgtcg
     7021 gacgtccgaa cgttggcaaa tccacattgt tgaacaaact gctggggcag aagatctcca
     7081 tcacctcccg caaggcgcaa acaactcgtc accgcattgt ggggatccat actgaaggcg
     7141 cgtatcaggc gatctacgtt gataccccag gcctgcatat ggaagaaaaa cgcgccatta
     7201 accgcctgat gaacaaagcg gcgagcagct ctatcggcga cgttgagctg gtgatttttg
     7261 tcgtagaagg cacccgctgg acgccggacg atgaaatggt gctcaacaaa ctgcgcgatg
     7321 gcaaagcgcc ggtaatcctc gcggtgaaca aagtggataa cgtgcaggaa aaagccgatc
     7381 tgctgccgca cttacagttc ctggcaagcc agatgaactt cctggatatt gtgccgatct
     7441 ctgccgaaac cgggctgaat gtcgacacta tcgcagcaat cgtgcgtaag catctgcctg
     7501 aagcgaccca tcacttcccg gaagattaca tcaccgatcg ctcacagcgt tttatggcgt
     7561 ctgaaatcat ccgcgaaaaa ctgatgcgtt tcctcggcgc tgaactgccg tactccgtga
     7621 ccgtggagat cgaacgtttc gtctctaacg aacgcggcgg ttatgacatc aacggtttga
     7681 ttctcgttga gcgtgaaggg cagaagaaga tggttattgg caacaaaggg gccaagatca
     7741 aaaccatcgg gattgaagcg cgtaaagaca tgcaggaaat gtttgaagcg cctgttcacc
     7801 ttgagctgtg ggtaaaagtg aaatccggtt gggccgacga cgaacgcgca ctgcgcagtc
     7861 tcggttatgt tgacgatctt taagagtaac tccgatggaa ggctggcagc gcgcatttgt
     7921 cctgcatagt cgcccgtgga gcgaaaccag cctgatgctg gacgtcttca cggaggaatc
     7981 gggtcgcgtg cgtctggttg ccaaaggcgc acgctctaaa cgctctactc tgaaaggtgc
     8041 attacaacct ttcacccctc tcttgcttcg cttcggtggg cgtggtgaag tcaaaacact
     8101 gcgcagtgct gaagctgtct cgctggcgct gccattaagc ggtatcacgc tttacagcgg
     8161 tctgtacatc aacgaacttc tctcccgcgt actggaatac gagacgcgct tctctgaact
     8221 ctttttcgat tacttgcact gcattcagtc acttgcaggt gccactggta cgccagaacc
     8281 cgcgctgcgt cgctttgaac tggcactgct cgggcatctg ggctatggcg tcaattttac
     8341 ccattgtgcg ggtagcggcg agtcggtaga tgacaccatg acgtatcgtt atcgcgaaga
     8401 aaaagggttt atcgcaagcg tcgttatcga caataaaacg ttcaccggaa ggcagttaaa
     8461 agcgttaaac gcgcgggaat ttcctgacgc agacacactg cgcgccgcga aacgctttac
     8521 ccgcatggcg cttaagccgt atcttggcgg taaaccatta aagagcaggg aactgttccg
     8581 gcagtttatg cctaagcgaa cggtgaaaac acattatgaa tgatgaggat tgtcatggct
     8641 gaattactgt taggcgtcaa cattgaccat attgcaacgc tgcgtaacgc acgcggtact
     8701 gcttacccgg atccggtgca ggcggcgttt attgctgagc aggcgggagc ggacggcatt
     8761 accgtgcatt tacgtgaaga tcgccgccac attactgacc gcgatgtgcg catcctgcgt
     8821 cagacgctgg atacccgcat gaatctggag atggcggtga ccgaagagat gctggcgatc
     8881 gccgttgaga cgaagccaca tttttgctgt ctggtaccgg aaaagcgtca ggaagtaaca
     8941 accgaaggcg gcctggacgt cgcagggcag cgtgacaaaa tgcgcgatgc ctgtaaacgt
     9001 ctggcggagg ccgggatccc ggtttctctg tttatcgacg ccgatgaaga gcagatcaaa
     9061 gctgcggcag aggttggcgc accgtttatc gagatccaca ccggttgcta tgctgatgcc
     9121 aaaactgacg ccgaacaggc gcaagagctg gcgcgtatcg ccaaagccgc gacctttgcc
     9181 gctagtctcg gtctgaaagt taacgctggt cacggtctga cctatcacaa cgtgaaagct
     9241 attgccgcca tccctgagat gcatgaactg aatatcggtc atgccattat tggtcgtgca
     9301 gtgatgaccg gactgaaaga tgcggtggca gaaatgaagc gtctgatgct ggaagcgcgt
     9361 ggctaatggc aatattaggt ttaggtacgg atattgtgga gatcgctcgc atagaagcgg
     9421 tgatcgcccg atccggtgag cgcctggcac gccgcgtatt aagcgataac gaatgggcaa
     9481 tctggaaaac gcaccaccag ccggtgcgtt ttctggcgaa gcgttttgct gtgaaagaag
     9541 cggcggcaaa agcgtttggc accgggatcc gcaatggtct ggcgtttaat caatttgaag
     9601 tattcaatga tgagctcggc aaaccacggc tacggctatg gggtgaggca ttaaaactgg
     9661 cggaaaagct cggcgttgta aatatgcatg taacgctggc agatgaacgc cactatgctt
     9721 gtgccacggt aattattgaa agttaaaaaa ataccgttct ctttataaag taaaaccccc
     9781 gaactggtaa ggaacggggg ttttcacttt acaactttaa ttttgtgcag cacacgattc
     9841 caacagaaat gattatggat caatttcgtt atcctgtaca agcaacttgc aatctctgac
     9901 ataaaaaacg cgagccttta cgaagaaatc gacattgata aaaagattaa cgtgaccgct
     9961 ttttgataag tacattaaaa tcggtttcgt tattggcaat tttgctgtgc tttacaccat
    10021 gccactgaat tcccccattg aaacgagtgg tgtcgtcaaa gctctggtgt ggagtgcagc
    10081 actcgattcc aataactcgc acgttcagtt ttggggtgat gtaagggcta atctgaacgg
    10141 ctgcattcct tgtttaagga aaaacgaatg actgattgcc gatacctgat taaacggatt
    10201 atcaaaatca tcattgctgt tttacagctg atccttctgt tcttataaca caaggaaacg
    10261 tacttaaggt gcgtccggtg aaccagtcgg acgcgccttt aataactata aataa
//
LOCUS       DAZUCC010000179        10299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZUCC010000179 DAZUCC010000000
VERSION     DAZUCC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.180"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            115..1023
                     /locus_tag="ACHY09_003906"
     CDS             115..1023
                     /locus_tag="ACHY09_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFS3886813.1"
                     /translation="MKAPVSITSFRPQKSIAIVIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1069..3060)
                     /gene="tkt"
                     /locus_tag="ACHY09_003907"
     CDS             complement(1069..3060)
                     /gene="tkt"
                     /locus_tag="ACHY09_003907"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS3886814.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3338..4096
                     /gene="loiP"
                     /locus_tag="ACHY09_003908"
                     /gene_synonym="yggG"
     CDS             3338..4096
                     /gene="loiP"
                     /locus_tag="ACHY09_003908"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFS3886815.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4144..5064)
                     /gene="speB"
                     /locus_tag="ACHY09_003909"
     CDS             complement(4144..5064)
                     /gene="speB"
                     /locus_tag="ACHY09_003909"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFS3886816.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5200..5931)
                     /locus_tag="ACHY09_003910"
     CDS             complement(5200..5931)
                     /locus_tag="ACHY09_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886817.1"
                     /translation="MKKWKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERVWRDPAEMIVISYSTTVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6077..8053)
                     /gene="speA"
                     /locus_tag="ACHY09_003911"
     CDS             complement(6077..8053)
                     /gene="speA"
                     /locus_tag="ACHY09_003911"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFS3886818.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8062..8193)
                     /gene="yqgB"
                     /locus_tag="ACHY09_003912"
     CDS             complement(8062..8193)
                     /gene="yqgB"
                     /locus_tag="ACHY09_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFS3886819.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8287..8430)
                     /gene="yqgG"
                     /locus_tag="ACHY09_003913"
     CDS             complement(8287..8430)
                     /gene="yqgG"
                     /locus_tag="ACHY09_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFS3886820.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8541..8792)
                     /gene="yqgD"
                     /locus_tag="ACHY09_003914"
     CDS             complement(8541..8792)
                     /gene="yqgD"
                     /locus_tag="ACHY09_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFS3886821.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8848..10002
                     /gene="metK"
                     /locus_tag="ACHY09_003915"
     CDS             8848..10002
                     /gene="metK"
                     /locus_tag="ACHY09_003915"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS3886822.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2562 a         2744 c         2594 g         2399 t
ORIGIN      
        1 tctaatgcaa tgttttttcg ctatacccct ttatagattc tgaattctca cctgtcggct
       61 attttctcct cacctcatgt gacatattcc ccactttact caaggaagat tcatatgaaa
      121 gcacctgttt caataacctc atttcgccca caaaaatcta ttgctatcgt cattggcgtg
      181 cttgccgtgg ttgtgctgcc atttctctcg tattacaccg ttaatgaagg tgaacgcggc
      241 attttgctgc gctacggaaa aatcgtaaaa gttgccgagc cgggtctggg ttttaagatc
      301 ccttttatgg aaagcgtgga gaaaatttcc acgcgtaatc aggctgtggt ttatcaggga
      361 ttacaggcct acagccgcga ccagcaaccg gcacaaatga cagtatcagt cagcttccac
      421 attaaacctt cagaagcagg cgcggtttat accacctaca acaccattga agcattaaaa
      481 gatcgtctga ttgtccgtca gttgccaact caactggaga acgttttcgg ccaatatacc
      541 gccatcagcg ccgtacagga tcgtacaaaa ctggttcagg atctacaaaa tgcaatgcgt
      601 aaagccgttg ttggcccggt ggtgattgat ggtgtccaga ttgaaaatat tgatttttct
      661 gacgcttatg aaaaatcgat tgaagatcga atgaaagcag aagtcgccat tgccacacgt
      721 aagcaaaatc tggaaacaga gaaaatccag gcgcaaatag cggtaactca ggcccaggca
      781 gaagcagaca gtaaactggc ggcagccaaa gcagaagctg agactattcg cgtaagaggc
      841 gcggcagagg ctgaaactat tcgtttaaaa agtgcggctg aagcagaagc gatccgcctg
      901 cgcggtgaag ctctgcggga taatcccgga cttgttgccc tcaccactgc tgagcgttgg
      961 gacggtaagc tacccgatac catgatcccc ggcagtacag ttccgtttat ctctactaag
     1021 taaaaaaagg gccgctttcg cggcccttct cacaaagcat cttaccgatt acagcagcgc
     1081 tttcgctttc gcaacaacgt tgtcaacggt gaaaccgaac tcttcaaaca gttgctctgc
     1141 aggagcagat tcaccgaagg tggtcatgcc gacgatagca ccgttcaggc caacatactt
     1201 gtaccagtag tcagcaatac ccgcttccac cgcaacacga gcggtaaccg ctttcggcag
     1261 tacggattca cggtaagccg catcctgctt gtcgaacgcg tcggtagacg gcatggaaac
     1321 cacgcgcgcc ttcacgcctt cggcagtcag tttttcgtag gcggcaacag ccagttcaac
     1381 ttccgaaccg gtcgcgatga agatcagttc cggctgaccg gcacagtctt tcagcacata
     1441 accaccgcgc gcgatgtttg ccagctgctc ttcagtacgc tcctgctgcg ccaggttctg
     1501 acgggagagg atcagcgcgg tcgggccgtc ctgacgctca acgccgtatt tccacgcgac
     1561 cgcggattca acctggtcac atggacgcca tgtggacatg ttcggggtca cgcgcagaga
     1621 agcgacctgc tctaccggct ggtgagtcgg gccatcttcg cccagaccga tggagtcgtg
     1681 ggtgtaaacc atcacctgac gctgtttcat cagcgcagcc atacgtacgg cgttacgtgc
     1741 gtattccacg aacatcagga aggtggaggt gtacggcagg aaaccaccgt gcaaggagat
     1801 accgttagca atcgcggtca taccgaactc gcgaacaccg tagtggatgt agttacccgc
     1861 agcatcttcg ttgattgctt tagaaccaga ccacagggtc aggttagacg gtgccaggtc
     1921 agcagagccg ccgaggaatt ccggcaacag cgggccgaaa gcttcgatag cattctgaga
     1981 cgctttacgg ctggcgattt tcgccggatt agcctgcagt ttagcgatga attctttcgc
     2041 tttggcatcg aagtcagacg gcatttcgcc tttcatacgg cgggtaaatt cagcggcttc
     2101 ctgcggataa gctttggcgt aagccgcgaa tttctcgttc catgcggatt ctttcgcctg
     2161 gcctgcttct ttcgcatccc actgagcata gatttcagac gggatttcga acggtgcata
     2221 tttccagccc agctgttcgc gggtcagggc aatttcagcg tcgcccagcg gcgcaccgtg
     2281 ggagtcgtgc gtaccggctt tgttcgggga accgaaaccg atgatggttt tgcacatcag
     2341 cagggacggt ttgtcagtca ctgcgcgcgc ttcttctact gcgcgtttga tggatgccgc
     2401 gtcatgaccg tcgatgtcgc gaataacgtg ccagccgtaa gcttcgaaac gcattgcggt
     2461 gtcgtcggta aaccagcctt caacgtgacc atcgatagaa ataccgttgt catcgtagaa
     2521 cgcaatcagt ttacccagct tcagcgtacc cgccagagag caaacttcgt gggagatgcc
     2581 ttccatcatg cagccgtcgc ccatgaaggc gtaggtgtag tggtcgacga tgtcgtggcc
     2641 cggacggtta aactgcgccg ccagcgtttt ttctgcaatc gccataccga ctgcgttggc
     2701 aataccctga cccagcggac ctgtagtggt ttccacgcca gcggtgtaac ccacttccgg
     2761 atggcccgga gttttagagt gcagctgacg gaagtttttc agttcttcca tcggcagatc
     2821 gtaaccggtg aggtgcagca ggctgtagat cagcatggag ccgtggccgt tggacagcac
     2881 gaagcggtca cggtcagccc aggacggatt ctgcgggttg tgtttcagga aatcacgcca
     2941 caggacttcg gcaatgtcag ccatacccat cggggcgccc gggtgaccgg atttggcttt
     3001 ctgtactgcg tccatgctca gcgcacgaat agcattggca agctctttac gtgaggacat
     3061 tttgactcca gatcggatga tgaagggcac gcccttaacg acttgacgac agcgcgtttt
     3121 gggctacgcc ggaaaatttg ccaacaattt accgcaagcc gcgcgtcatg tacatggaac
     3181 atccttttgc cgcttcagaa atctctggat catgctcgca tgttgcgcaa tctgctcgcc
     3241 cgtccgctgc gcttttcctt atactgagac tgagcgtcga ttcacctgca aacggcgcat
     3301 ttttagaata atcctgacct tgtgcggaag agaaaacatg aaaattcgcg ccttattggt
     3361 agcaatgagc gtggcaacgg tactgaccgg ttgccagaat atggactcca acggactgct
     3421 ctcatcagga gcggaagctt ttcaggctta cagtttgagt gatgcgcagg tgaaaaccct
     3481 gagcgatcag gcatgtcagg agatggacag caaggcgacg attgcgccag ccaatagcga
     3541 atacgctaaa cgtctgacaa ctattgccaa tgcgctaggc aacaatatca acggtcagcc
     3601 ggtgaattac aaagtgtata tggcgaagga tgtgaacgcc tttgcaatgg ctaacggctg
     3661 tatccgcgtc tatagcgggc tgatggatat gatgacggat aacgaagtcg aagcggtgat
     3721 cggtcacgaa atggggcacg tggcgttagg ccatgtgaag aaagggatgc aggtggcact
     3781 tggtacaaat gccgtgcgag tagctgcggc ctctgcgggc gggattgtcg gcagtttatc
     3841 tcaatcacaa ctcggtgatc tgggcgagaa attagttaat tcgcaattct cccagcgcca
     3901 ggaagcagaa gccgatgatt attcttacga tcttctgcgc caacgcggca tcagcccggc
     3961 aggtcttgcc accagttttg aaaaactggc gaagctggaa gaaggccgtc aaagctcaat
     4021 gtttgacgac catccagcat ccgccgaacg cgcccagcat attcgcgatc gtatgagcgc
     4081 ggatggagtt aagtaagcat tttattacgc cggatttgac gcataacgca tcttatccgg
     4141 cgcttactcg ccctttttcg ctgcctgaat atacagcatc tccagcgcca gcgtcgccgc
     4201 agccagagcg gtgatttccg actgatcgta tgccggagcc acttccacta cgtccatccc
     4261 aacaatgttg agatctttca ggccgcgtac cagtttaata gcgcgatcgg aggtcaggcc
     4321 gccaattact ggcgtaccgg ttcccggcgc aaatgcagga tccaggcagt cgatatcaaa
     4381 ggtcaggtaa accggcatat cacccacaat ctgtttcact tgggcgataa cgtcatccac
     4441 gctgcgatcg ttcacctggc aggcgtccag cacggtaaag ccgttgtctt tatcgaactc
     4501 agtacgaata ccaatctgca cggaatgatt cgggtcgatc agaccttctt tcggcgcggt
     4561 atagaacatg gtgccgtggt caaattcaca accgttcgca taggtgtcgg tgtgggcgtc
     4621 aaagtgtacc agcgccattt tgccgaaatg cttcgcatga gcacgcagca gcggcagcgt
     4681 aacaaagtgg tcaccaccga aggagagcat acgcttaccg gcagccagca gcttctcggc
     4741 gtgcgcctgc agcttctcgc tcatctcacg ggcatctccg aaggcatata ccagatcgcc
     4801 gcagtccacg acgttcaggc gctcacgcat gtcgaaattc cacgggaagc gattgtgttc
     4861 ccaggccaga ttcgtcgaaa cctgacggat cgctgccgga ccgtggcgac cacccgcacg
     4921 accagaagtg gccatatcga acggcacgcc agtaatcacc cagtcagcat cgctgtcata
     4981 tggctggaag ttcatcggca ggcgtaaaaa accaaaggcg ttggaaacca gtgagttatc
     5041 gtattgatga cctaaggtgc tcatggatgg actcctatta caaagtcgat ataaaaaaac
     5101 ccttccgcgt cgttaggccc gacgaggaag ggttggattt gcgaaacaca attttgtgct
     5161 aattatcgcc ttaaatgggc gaggcatcaa gcgaggttgc tatttccgac acggtggcgg
     5221 cgcatagcca ttgttatctg ccggaaaggc ttgtccagag cacggcggca gcgcgttttg
     5281 cgccgggcct tgtaattcaa aggcggcaaa gccgattaca ccggtattac gcacatcgcc
     5341 ttgcgccgaa ttggctgcgt aggaatcagc aacgttggcg aactgaaaag ccgcttcggt
     5401 atgtttatct ttgcggaatc ctttaatcgc taacgaatct ccggcattca caatatagcc
     5461 gttgttattc agcgaacctt gcttgccgtt cagcacatcc aggccatcaa ccgtagcaac
     5521 gatctcgtag ttggtattac gactgtaatt acggatatat aattgataat tcattccgac
     5581 tctggcatgg acaatatagc gtggttcatt attggatgaa tcataaaccc gcgtaatagg
     5641 tagagaatta aaattaccat cgcgaatagc atattccagt tcgttaatac gaatgctata
     5701 aacgcggtca tagccggaag gaacagtagt cgaataacta ataacgatca tctctgccgg
     5761 atcgcgccag actcgttcag cagatacgcc ctgaaccgat gagtgtacat ccccacccca
     5821 ggcggtgccg acattgcctg atgcactgct attatactgc gcattactgg aacaaccggc
     5881 cagcagcacg gctaatgcta tcaacgcgct acgtactttc cattttttca taaccgttcc
     5941 ctctgcattt aattaatgca gcgattgtag ggggattatt taataagaaa agcagctaaa
     6001 ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa tcgacccgcc
     6061 agcggaataa tcttaattat tcatcttcaa gataggtata accgtacaaa cctgcctcga
     6121 actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc acttgatcac
     6181 ggaactgggt taacaacgtt ttcggatcga gctgtacata ttgcagcata tccgccacgg
     6241 tatcgccttc gtcagacagt tctacttcta cgctgccgtc agggaagacg aacacgtcaa
     6301 ccgcttcggt atcaccgaac aggttgtgca tgttgccgag gatctcctga tatgcgccga
     6361 ccatgaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt ggcattgtcg
     6421 tggcaatacc atcaccatcg atgtagtgat caatagcacc gtcagaatca caggtaatat
     6481 ccagcagcac agcgcggcgc tccggcactt gatccaaccc ttccagcggc agaaccggga
     6541 acaactggtc gatcccccat gcgtccggca tcgactggaa cagcgagaag ttgacgtaca
     6601 ttttgtcagc catacgttcc tgcagctcgt cgataatcgg acgatgcgca cggttttgcg
     6661 gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct gcccatgcac
     6721 gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatatcgtgc agatccatct
     6781 ggctgtcgtg taaccattca cgcagagaac gacgagttcc cggttcgtgc atctcctgcc
     6841 aggtttccca catgctttgc agggcgcgcg gtgcgtcttc tgcaggcgcg gtcggcaccg
     6901 tgtattcgtt acgttccacg ccgatgatat tcgacaccag cacggtgtga tgcgcagtca
     6961 ccgcacgacc cgactcggtg attaccgtcg gatgtggcag accattttct tcacacgcat
     7021 caccaatcgc ccagatgatg ttgttggcgt attcattgag gccgtagttc accgaacagt
     7081 cggactgcga acgggtgcct tcatagtcca cgcccagacc gccgccaacg tcgaagcact
     7141 gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca cgaacgcctg
     7201 tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagc agttgcaggc
     7261 tgtcgagacg cccggcttca cgcagcgttt caaccagttg cagtacctga gtcgccgcca
     7321 ggccgaactt cgatttttcc ccgccagagg actgccattt acccgagccc tgcgaagcca
     7381 gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca tccagcacaa
     7441 tggcaatttc tgacatcttc tcaatgacca gatagacctt gtggcccatc ttctcgccaa
     7501 ttaatgccag gcggatatat tcgcggtcct tataaccgtt gcagacgatg acgctacggg
     7561 tcatgccagc atgtgccagc accgccatca actccgcttt ggaaccggct tccaggccca
     7621 gcggttcgcc cgaatgaatc agggactcaa tcacgcggcg gtgctggtta actttgatcg
     7681 gataaacgag gaagtaatcg ccgttatagc cgtaggattc ccgcgcacgt ttgaacgcgg
     7741 cgttaataga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt gcaggcagac
     7801 gctggccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga gcttccggga
     7861 cgtccgggtc cgggcacacg ctaatgtggc ccagctcgtt aacgtcatag tagttattgc
     7921 cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag ctcattgcaa
     7981 cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa cccatagaca
     8041 tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt taacgactat
     8101 cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca gtgaatgctg
     8161 acgctccaac tgcgcaaccg gtttcttttt cataacatta ttaagcacat aaccgaacgt
     8221 aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag cgcccttgtt
     8281 cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat gggtataaca
     8341 tcggcaggta tgcaaagcag agatgcagag tgcggggaac gaatcttcac cagaacggtg
     8401 agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg gccgcctgtt
     8461 aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc aacacgaaag
     8521 aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac aatggcagaa
     8581 aattgcaaaa gataaatacg cagaatgcca gcattgtcag gaaaaatttc cagccacgtt
     8641 tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa ttgacctaaa
     8701 atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc agtgttttta
     8761 acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag tttgagctaa
     8821 ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc cgagtccgtc
     8881 tctgaagggc atcctgacaa aattgctgac caaatttctg atgccgtttt agacgcgatc
     8941 ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac cggcatggtt
     9001 ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat cacccgtaac
     9061 accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa ctcctgtgcg
     9121 gttctgagcg ctatcggcaa acagtctcct gacatcaacc agggcgttga ccgtgccgat
     9181 ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac caatgaaacc
     9241 gacgtgctga tgccagcgcc tatcacctat gcacaccgtc tggtacagcg tcaggctgaa
     9301 gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca ggtgactttc
     9361 cagtatgacg acggcaaaat cgttggtatc gatgctgtcg tgctttccac tcagcactct
     9421 gaagaaatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat caagccgatt
     9481 ctgccggctg aatggctgac ttctgccacc aaattcttca tcaacccgac cggtcgtttt
     9541 gttatcggtg gcccgatggg tgactgcggt ctgactggtc gtaaaattat cgttgatacc
     9601 tacggcggca tggcacgtca cggtggcggt gcattctccg gtaaagatcc atcaaaagtg
     9661 gaccgttccg cagcgtacgc agcacgttat gtcgcgaaaa acatcgtggc tgctggcctg
     9721 gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc gacttccatc
     9781 atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct gctggtacgt
     9841 gaattcttcg acctgcgccc atacggtctg attcagatgc tggatctgct gcacccgatc
     9901 tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg ggaaaaaacc
     9961 gacaaagcgc agctgctgcg tgatgctgcc ggtctgaagt aatctttctt cacctgcttt
    10021 caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggac aggcattcac
    10081 gccgcatccg gcaaaaaaac cgcccgcaca ataacatcat tcctcctcaa cacgttttac
    10141 cgcaaattac catcactact gaaaccgatt acaccaacca cgccagccaa atatttgtaa
    10201 tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt aaagtcttaa
    10261 ttgcagataa cagcgtttaa tctatgatga tataactca
//
LOCUS       DAZUCC010000180        10225 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZUCC010000180 DAZUCC010000000
VERSION     DAZUCC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.181"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..961)
                     /locus_tag="ACHY09_003916"
     CDS             complement(<1..961)
                     /locus_tag="ACHY09_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS3886823.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYTELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAA"
     gene            complement(1110..1307)
                     /locus_tag="ACHY09_003917"
     CDS             complement(1110..1307)
                     /locus_tag="ACHY09_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS3886824.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(1523..1903)
                     /locus_tag="ACHY09_003918"
     CDS             complement(1523..1903)
                     /locus_tag="ACHY09_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFS3886825.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(1921..2910)
                     /locus_tag="ACHY09_003919"
     CDS             complement(1921..2910)
                     /locus_tag="ACHY09_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS3886826.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLEAMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRKDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(2962..3219)
                     /locus_tag="ACHY09_003920"
     CDS             complement(2962..3219)
                     /locus_tag="ACHY09_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886827.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(3216..4616)
                     /locus_tag="ACHY09_003921"
     CDS             complement(3216..4616)
                     /locus_tag="ACHY09_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS3886828.1"
                     /translation="MTNPTNPAWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFF
                     VRQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWAKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            complement(4613..5503)
                     /locus_tag="ACHY09_003922"
     CDS             complement(4613..5503)
                     /locus_tag="ACHY09_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS3886829.1"
                     /translation="MTNFLNKQHMQHDKAQLLNRKADLEEELAFVRGGKRPWRCEHWV
                     QSTETVSCGKHGKYTRYVLTGPDVRGKAVKRVSGCLSCLSEELDRVYDELRALKVREL
                     LDQAGIARRFQDCEFENYQAVNTDAQKNLEACQRYAASWKKCLAAGTSMVMIGNCGTG
                     KNHLAVSMAKNIIRHHQATIEITDVMRLTRAVKNTWRHNSERTDDEVINHFASLDLLI
                     IDEVGVQFGTPTEITILQEIINARYESVLPTILISNLTFEQLKESIGERIVDRVTDGG
                     RNCLVFGWESYRAHIRGVAA"
     gene            complement(5523..6434)
                     /locus_tag="ACHY09_003923"
     CDS             complement(5523..6434)
                     /locus_tag="ACHY09_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3886830.1"
                     /translation="MSSKILGNVWDACAAYGIKGAKLMIMARLADYSNDDGVCYPGVE
                     TICRQLGLGESTVRTAISELEADGWLTRQSRRKGNRNTSNLYHLNADRLELLARTERD
                     KVAELKQQRRLSGLRDPSDSEPSKSEPSESVCSGVFDPSDSGKNTRLTLQNLTPDPQG
                     LKHEPPVNSKHEPQDIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFK
                     AWKARIREGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEESWQQPA
                     APGGGRGKSLPISGFSEQDYGSTNFNW"
     gene            complement(6431..6655)
                     /locus_tag="ACHY09_003924"
     CDS             complement(6431..6655)
                     /locus_tag="ACHY09_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886831.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGHVLRVCLVNIESQKVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGAV"
     gene            complement(6652..7515)
                     /locus_tag="ACHY09_003925"
     CDS             complement(6652..7515)
                     /locus_tag="ACHY09_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS3886832.1"
                     /translation="MTRHQHIDSASPGRYTVPAPYKTGAGIGVLKLITEHNRAHAVFS
                     CHEHYYAQIMVGRTGPTSVGPGSVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMT
                     TLPTLSQPEIAIVDGQAVTSSLAVADFFSKRHDDVLKKIRILDCSPEFCARNFAETSI
                     LVRQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMEKSLSGADAS
                     DMSAVARNARGVYLHLREIHQIWTSQLYPMLKAVESPLASKLYDRVGDAVFGAALVDS
                     RLNGSDKEVRP"
     gene            complement(7512..8147)
                     /locus_tag="ACHY09_003926"
     CDS             complement(7512..8147)
                     /locus_tag="ACHY09_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFS3886833.1"
                     /translation="MKFRRHTLHIVAKSTIGSETPELIEATNDAPASFLLSVLAHTRI
                     MVGWAGEPKGSPVSLIAGSLNPVQSATSEIETSCGGYCHSPIKEAALMATIPAHPYLK
                     IEVVNGRAVIFSLYVACHFKRMHKNIIDRIEYLNCSREFLTRNFIPGTYRAYGDSVRG
                     YYITRDGLMMLQLGLSLRTMRYYESCIEAFHEAEAVQGHSVFRRNQREVHQ"
     gene            complement(8176..8352)
                     /locus_tag="ACHY09_003927"
     CDS             complement(8176..8352)
                     /locus_tag="ACHY09_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS3886834.1"
                     /translation="MQVLNRWFRDGRGRRVHVIRWESETQRVIYLRDGYPHECFSPLW
                     LFRRDFVECEAPPT"
     gene            complement(8372..9079)
                     /locus_tag="ACHY09_003928"
     CDS             complement(8372..9079)
                     /locus_tag="ACHY09_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS3886835.1"
                     /translation="MNTTIFTDKASMTSVEIAELVGKRHDNVKRTIETLAKGGVVRSP
                     QIEVSERINNLGFKVQHEHYLFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRLERVEEKVSHVTETVEQIKRGAIRDGYAGYRQLAAK
                     TGMSDAKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMSAFHRMMSEAEPRGT
                     RWYHPKMGLFQALGWQV"
     gene            complement(9076..9339)
                     /locus_tag="ACHY09_003929"
     CDS             complement(9076..9339)
                     /locus_tag="ACHY09_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3886836.1"
                     /translation="MKNEGIAKAVDIAGSQIALARRCGRAQSTICDWLNGKKKISPEF
                     VPSLVKAVDGKVQAYEFRPDLPELFPHPSLAHTEDGVCEGDKQ"
     gene            9463..10167
                     /locus_tag="ACHY09_003930"
     CDS             9463..10167
                     /locus_tag="ACHY09_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS3886837.1"
                     /translation="MSFSDRLQLAMTIRGYSQGKLAREVGMAQSSVNKLVNGATGSRK
                     VVEIANTLNVRPEWLSYGVGPMTPEESTSCNPTPQVSYSPLENDKDIFRVEVLDIAAS
                     AGPGTFLVSDFAETVHAIEFSHDAARRLFCSRPANIIKMITVDGDSMAPTLCAGDQVF
                     VDVSVRNFETDGIYIFIFGHTFHIKRLQMQGMQLAVISDNPAYKEWFISESAEEHLFI
                     MGKVLIHQSIQYNRVG"
BASE COUNT         2448 a         2820 c         2436 g         2521 t
ORIGIN      
        1 tcgctgcttt gactgtcgaa cccgacccca tgaaaaaatc tgcaaccagg tcacccggac
       61 gactgctcgc gctgattatc tgctgcagca tttctgccgg tttttcgcac ggatgtttcc
      121 cgggatagta ctgcaccggt ttatgcgtcc acacatccgt gtacggcacc tgcgccgtca
      181 cgccaaaata ccgccgcaga tgcttatatt cactctgcag ttccgtatac tgccggttca
      241 gtgaagtata cgtctccagc agctggtggt ggggcttttc cagttcaccc cgctgatgct
      301 tctcttctgc cacccgggca aacagcgcct gtaatttcag ataatcactt tcgttcggca
      361 gctgccactg gctggcactg aaccagtgcg acaccatgtt tttctttcct gtggcatcag
      421 caatctgttt tgccgttatc cccagagcgg cacgcgcatc acgaaagtaa gatatcagcg
      481 gggccatcac atgctgtttc agtgccctgc ccttcgcctc atagccatca tctttcgggc
      541 gatacggccc ctgatagtgt tccgcgaaca gaatgcgctc tgtggcgggg aaatacgccc
      601 tcaggctttc cttgttgcac ccgttccagc gtccggacgg cttcgcccag ataatatggt
      661 tcagcacact gaagcgttca cgcatcatga tttcaatgtc agacgccagg cgatggccac
      721 agaacaggta aagacttccg gcaggtttca gcacccgcca gaactgcgcc agacactggt
      781 ccagccactt caggtaatca tcgtcgccct tccactggtt atcccagccc tcaggcttca
      841 ctttaaagta cggcgggtcc gtgactatca ggtcaacaga attttcgggt aacgaccgga
      901 taaattccag gcagtcggcg ttgattaact cacaactgga tatttttaca gtattaagca
      961 tggatcatta agcctgtctc tgataggccc actgcttttg cgcaaagcag tgggcctgag
     1021 gtttgcttgt gatccggacg catgagcaga tggctggtga gtgcccctaa cacccaccag
     1081 ccgcccattt accacaaata aaaaagcctt caggactgaa ggcgtctgta acaaccgaac
     1141 tgatagtctg ccagccccgc cataacaagc tgggtcagta ttaactggca gcgttcacgt
     1201 gaaaggtacg tattctgcgc aatctccccg actgtcgccg gttcggtgac gcttaattca
     1261 ttaaacacca ctctggcggt ttctgtcata tcctgctgtt tcagcatgtc tttttccctt
     1321 ttccggttaa cgtgacacac caataactct tgtcgaaaaa gccagcaagc tgaaagaccg
     1381 gtattcaccg ccaccagcgc gtttactgta ctggcgtgat ttcagtcata aaaaaacccg
     1441 ctcgcggcgg gtttaagctg tgtggcgaag taaccactct taacatactg acatactttt
     1501 tgcggaccgc actaatcatt ttttactttt ttagcagcca gtcgtccatc tccagtctta
     1561 cccccagcac agacaaacat ccgtcaataa acccttcggc tatctgcatc tcaattcgta
     1621 ttgctttttc gcttttcttt ctcgtcctgg ctatctgtct ttttgatatt cgcaacaaat
     1681 aatgagcaat gagaagcgaa tactcctcag gttttttctg cttcagacga gcaagacagt
     1741 tttcaatgat aagtccgtca tcatcgcagc aggccggacg tggtttagtg gcagatggta
     1801 aaagtccttt gaatccggca gcgatcggag aatagtccac cccggtgtta ccacttgcag
     1861 cccatgcccc ccagcgttca agaaccatct gaatatcacg catcaacttt ctccacaaaa
     1921 tcaggacagc acaccaatcg ccagcgcgcg atcgataaaa cgaaatatca gctccagttg
     1981 ggaaccatac ttctcttcaa atgccacggt atccgcatgc agttcgtcat ggtgttttct
     2041 gcacaaaggc aacacaaaaa ggtcatgcgc ttttgtaccc attccaccct gaccatgacc
     2101 aatcaggtga tgaggatcgt cggctggctt accacaacat gcacacggct gcgtcttaac
     2161 ccagcgcgtg tacttttcat taacccagcg gcgacgtttt gggcgtaaca taaaagactc
     2221 cggcgactcc ggatccactt tcagcgccag cacctttttc gccttatcct ggatgatgct
     2281 ggtggcagga accgaaggca caaggtcact ttcccgggtg acagacggca caacaggctt
     2341 cggtaatctc agtgccttac gggctgcact ttccggtaag gcatccgcca ggtccttacg
     2401 aatcagccac cagcacagtt ccggcattgt cacaacgtga ctgtcatcaa aaccaagatc
     2461 acggcgcaca acagacaaca cccagcgggc acagttatcc gttgccattg cttccagccg
     2521 ttccgtgaac tggtcacgga gaagattgtc acagtgccag cacagacgga ttgcgcctgg
     2581 cgcgtgccgc attgtggtca tgttctcgct gtgccagtcg gaatgaggcc actgacagcc
     2641 tttttcacga agtaaccagc tctcaaggca ttccacgcca ccagcacgac ggatcaccgc
     2701 ctcatggcgg aacacggccc gaacggcagg atcatccgcc agcggttgtg atgctgccgg
     2761 aacggcacca ctggcaaaag atgaataacg ttccggctct ggctccagca ggacacgccc
     2821 ctgcataaac aggggcatca gctctgaacc gggtctgaac aagacgatcc ccatacgcgg
     2881 ggcaatttca ggggtcagca gtgctctcac ggtcacctca gcgaacggta ttgcatgaac
     2941 gcaggagaaa aaattcagcc atcacgcagt aaactctttc accagcgttt caaactggct
     3001 tacctggcct tccagttccg ccacgcaatc caccagctca tccaccgcct tttgtgtgcg
     3061 atgttttgcc tgcagcagat cacgaagcgc cggagtaagc tgcttgcgga gcgtatcttt
     3121 tgccacgctc gttttttcca tctgttcagc acaacgaagc atctcctgcg cctgccgacg
     3181 aagttgttcc ggtgaaacag tggttgttct gttgttcaaa ataaacgctc cgttttacta
     3241 cccgacatgc ggttattgct gtatctgcgc ggattgcccg gcgtcatggg agtggaaaga
     3301 acccgggcac tctcctggtc cacaggcaga aaatgcccgt tatgaaaacg ccggtaaatg
     3361 gtacccagcg tgccattacg ctgtttcgtg atgttgattt cagctatgcc tctcgcctgt
     3421 gtctccgggt tgtacacctc atccctgtaa agcatcagaa tgatgtctgc atccgcctct
     3481 atttcccctg agtttttcag gtccgagttc atggggcgtt tattgggtct ggattccacg
     3541 ccacgggaga gctggctcag agcaatcagc ggaaaaccgc cggattttgc caggctttta
     3601 agtccctttg agatttcccc cacagcaagg tcgtgacgcc ccgtgctgcg ggttttaatc
     3661 aggccgaggt aatcgaccac caccagcgcc gtttccgggt gtttcatccg gtggtgcctc
     3721 gtggttgcac atatctcatc aatggtcagg tttgcctggt ccaccatcca gatattacgc
     3781 cccgtcattc gtcccacgcc ctgtgagaaa cgcgcccagt cttcatcttc aaaacgggca
     3841 acagacttaa gacgggatac cggcattccc ccggcagcag acaccatacg ttcaccaatc
     3901 tgaatgttcg ccatctccat ggtgaacaga agcacgccat gcccctgctc agtcaccttg
     3961 tcgatgatat ccagcgcaag ttcggttttc cccatcgaag gacgagccgc aatgaatacc
     4021 aggtcgcctg gctccatacc gcccgttttt gcgtccagtt catcaatacc ggtcatcagc
     4081 gccctggatt tctccagtcc ctgattgcgg cattcaacac ggtcgaccac ttccggaagg
     4141 acatcatcaa tgtgaaccgg ctgaatgacg ccctttccgg tcgacagtga ggccatcatg
     4201 ttctgcgcat ccttcagggc atcctcagct gcttcacagg tatgcgcatc acgtaaattc
     4261 tgtaatgctt cggtcagtgt tttttctgca tcgcgcagtg cggcattgcg ccgcagcgct
     4321 gcaacatagt gctccagtga agacttcgcc caggttttgc gtccggtgtc ggtaatcacc
     4381 ggggcaagtt ccggcatctc attgcacagc agtacggggt caatgacgcc ggatacacgg
     4441 gcctgtctgc aaattcccgc gtaaatatcc ctgtactgac gcacgaaaaa gacatccgcc
     4501 ggaagtgtgg ccagaatatc catcacttcc ggatcagccc cacgcagaaa aaacgcgcca
     4561 atgacagctc cttccaggtc atcattacgc cacgccggat ttgtcgggtt tgtcatgctg
     4621 ccacacctct gatatgcgca cggtagcttt cccaaccaaa caccaggcag ttacgcccac
     4681 catcagtaac gcgatccaca atccgttcac caatggattc cttaagctgt tcaaacgtca
     4741 ggttgctgat caaaattgtc ggtaaaacgc tttcgtaacg cgcatttatg atttcctgca
     4801 ggatggttat ctctgtaggc gtgccgaact gcacaccaac ctcatcgatg ataagcagat
     4861 ccagcgatgc aaaatgatta atcacttcat cgtcagtgcg ctcagagttg tggcgccagg
     4921 tatttttcac cgcacgggta agccgcatca catccgtgat ttctatggtt gcctggtggt
     4981 ggcgaataat attttttgcc attgatacag ccaggtgatt tttcccggta ccacaattgc
     5041 caatcatgac catgctggtg cctgcagcga gacatttctt ccaggaagcg gcatagcgct
     5101 gacaggcttc aaggtttttc tgtgcgtcag tattcaccgc ctgataattc tcaaactcac
     5161 agtcctggaa ccggcgggca ataccggcct gatcgagtaa ttcacgaacc ttcagggcgc
     5221 gtaattcgtc atacacgcgg tccagctcct cactgaggca ggacaggcaa ccggacaccc
     5281 gtttgacagc tttccccctt acatccgggc ccgtcagcac atagcgcgtg tattttccgt
     5341 gtttcccgca ggacaccgtc tcagtgcttt gcacccaatg ctcacagcgc cacggacgct
     5401 ttccgccacg gacaaatgcc agctcctctt ccaggtctgc cttgcggtta agtaactgcg
     5461 ctttgtcgtg ttgcatgtgt tgtttgttca ggaaattagt cattttcacc ctccccacaa
     5521 aatcaccagt tgaagttcgt tgagccgtag tcctgttcac tgaatcccga gatcgggagg
     5581 cttttgcccc gcccacctcc gggggctgct ggctgttgcc aggattcctc gaaatgacga
     5641 tcgggtccaa agaacgtcga cgcctgcttc acgaactggg taccggtatt tccagagaca
     5701 cgcacccagg cggcatagcg tttcacaccg ttgagcatgg tttcgggtgt cacaccttcc
     5761 ctgattcggg ctttccaggc tttgaaggct gctgacttgg aattaccacc agcacgtttg
     5821 ggatattcct gccaggcctg ttcaaattcc ggtgaatatt cctgtctggc agaacgcgct
     5881 ggtgcagacg cgtcagcgga tgcgccaata tcttgcggtt catgttttga atttactggt
     5941 ggttcatgtt ttaaaccttg tggatctggg gtcagattct gaagggtcaa acgcgtattt
     6001 ttgccagaat ctgaagggtc aaacacacct gaacatacag attctgacgg ttcagatttt
     6061 gaaggttcag aatctgaagg gtcacgtaat cctgaaagtc tgcgctgctg tttcagttct
     6121 gcaaccttat cccgctcagt tctggcaagc agctcaagcc gatcagcatt cagatgataa
     6181 agattggacg tattacggtt acctttgcgg cgtgactgac gcgtcagcca gccatcagcc
     6241 tccagttcgg agattgccgt tctgactgta ctttctccca acccaagctg tcggcatatg
     6301 gtttcaacac ctgggtagca caccccgtca tcattcgaat aatccgccaa gcgcgccata
     6361 atcatcagtt ttgcaccttt gatgccatat gctgcacatg catcccagac attacctagg
     6421 attttgctac tcatacggca cctcccagac gcttaaacat ttttccggac tgaaacgcca
     6481 ccagcggata actcagggta tgagtacgtc cctgaacctg gcagacaacc ttctggcttt
     6541 ctatattgac caggcaaacc cgcagaacgt gtccgttgct ggtggtgaac cactgcccca
     6601 cacgggggca atggttgtat cggtgataca ggaaattaac gatgtggcgg atcatggacg
     6661 cacctccttg tcagaaccat tcagcctgga atcaacaagt gcagcaccaa aaacagcatc
     6721 acctacacgg tcgtacagtt tgctggccag cggagattca acagccttaa gcattggata
     6781 aagctggctt gtccagattt gatggatttc acgcaaatgc aggtatacgc ctctggcgtt
     6841 tcgtgcgaca gctgacatat cagacgcatc ggcacctgat aaactcttct ccatctggtt
     6901 aaaggcattg atgtatgcct ctttgaaccg ggcagcacgt ttaccagtga agcccatagc
     6961 aagaaacgca aagccgtcgc gggttatttg atagcaagga agtttgcgag taccgccgtt
     7021 gggctggcgt accaaaattg atgtctccgc aaaattgcgg gcacaaaact ctggagaaca
     7081 atccaaaatg cggatctttt tcagaacatc gtcatgacgt ttagagaaga agtcagcaac
     7141 agccaaagaa gatgtaacag cctgaccatc aacgatggca atttcaggtt gagagagggt
     7201 tgggagagta gtcatagtga cagccccggt agtcagtttt ttagaaaact caccacatgg
     7261 gacgccaatc acagaggtgg tgagacgtac agggttggcg ttaccggaga ctaccgaacc
     7321 cggcccgacc gaagtcggcc ctgtacgccc caccataatt tgggcgtagt aatgctcatg
     7381 acacgaaaaa accgcatgag cgcggttatg ctcagtaatc aatttcagga cgccaatccc
     7441 ggcacccgtt ttataaggtg ccggaacagt gtaacgtccc ggacttgcag aatcaatatg
     7501 ctggtggcgc gtcattgatg cacctcccgt tgattacgac ggaacacgga gtgaccctgg
     7561 acggcttcag cctcatggaa tgcttcaatg cagctctcgt agtaccgcat ggtgcgcaga
     7621 cttaacccaa gctgtagcat catcagacca tcacgggtga tgtaataacc acgcactgaa
     7681 tcgccatagg cgcgataagt gccgggaata aagttgcggg tgagaaattc gcgtgagcag
     7741 ttgagatatt cgattcgatc gatgatattc ttatgcatgc gcttaaagtg acaggcgaca
     7801 tacagggaga atataacggc tctgccgttg acgacctcga ttttgaggta tggatgtgcg
     7861 gggatcgtag ccattaaggc agcctccttg atcggtgaat gacaatatcc accacaggag
     7921 gtctcaatct cgctggtggc ggactggaca gggttgagac taccggcgat caaggaaacc
     7981 ggcgaacctt tcggttcccc tgcccagccc accataattc tggtgtgcgc aagcactgac
     8041 aacaaaaaag acgctggcgc gtcgtttgtc gcctcgatca attccggggt ctcactcccg
     8101 atagtggatt ttgccactat atgtaaagtg tgccgccgga atttaatttt gtcaacgagg
     8161 ttaaatatag agtcgctatg ttggtggcgc ttcgcactca acaaaatcac gacgaaacaa
     8221 ccacagcggg ctgaagcatt catgcggata accatcacgc aggtaaataa cccgctgtgt
     8281 ttcagactcc cagcgtataa cgtggacgcg acgccccctt ccatcacgga accagcgatt
     8341 gagtacttgc atgtgttacc tgtgagcata attacacctg ccagcccagc gcctggaaca
     8401 accccatttt cgggtgatac cagcgagtac ctcgtggttc tgcttcactc atcatgcgat
     8461 gaaaagcaga catgaagggt tccacagcaa caatagcgcg acgcgacaac aatccatccg
     8521 gcgtcatgaa ctcatgggtg tcggtaggga tctgatatgc gttcaccaga ttgcggcatt
     8581 tcgcatctga catacccgtt ttcgccgcca gctgacgata gcctgcataa ccatcgcgta
     8641 tggcgcctct tttgatttgt tcgacagttt cggtaacgtg actgactttt tcttctaccc
     8701 gctcaaggcg tttctgctga cgaacagctt caagagccat cgcggcaacc atttcgattt
     8761 cgctcattgg cttgcggatc tgttcttcca gttcgcgcca gcggtctacc agacgggcgg
     8821 tgaattcagg gcaaagctgt gcgacaacaa tgatgctgtc gcgtttgcct tgttcgcctt
     8881 caaacagata atgctcgtgt tgaactttaa accctaagtt attgattctt tcggaaacct
     8941 caatttgagg agatcggaca acaccacctt tagctaatgt ttcaatagtg cgtttcacat
     9001 tgtcatgacg cttacccacc agctctgcga tctcaacgct ggtcatggat gctttatcgg
     9061 taaaaattgt tgtattcact gtttatctcc ttcgcacact ccatcttctg tgtgcgctaa
     9121 acttgggtgt gggaaaagct ccggtagatc aggacgaaat tcatacgcct ggactttccc
     9181 atcgaccgcc ttaaccagag acggaacaaa ttcaggagaa atttttttct tcccattcag
     9241 ccagtcgcaa attgttgact gcgcgcgacc acaacgccta gccagagcaa tctggcttcc
     9301 cgcaatatct acagccttgg ctataccttc atttttcata tcgacctcca acctgattcc
     9361 attcactata gcgattgatt aaagcgattg caatgtacgg gaaagcggtt tgtgtatata
     9421 tcgctaaggc gatacactaa aagggcagaa aaaggaggac ttatgagctt ttcagacagg
     9481 ctacagttag ctatgactat ccgcggatac tcgcagggaa aactggccag agaggttggc
     9541 atggctcaat cgagcgtaaa taaactggtt aatggtgcca caggctccag gaaagttgtg
     9601 gagatagcca ataccctgaa cgtgcgcccc gagtggctat cgtacggtgt tggtcctatg
     9661 accccagaag aaagcacatc ctgcaatccg acaccacagg tatcatattc cccgctggaa
     9721 aatgataagg atatttttcg tgtggaagtc ctggatatcg ctgccagcgc gggaccagga
     9781 acattccttg tttcagattt tgcagaaacg gtccatgcta tagaattttc acatgacgcc
     9841 gcaagacgtc tgttctgtag caggccagca aatatcataa agatgataac agttgatggc
     9901 gacagcatgg ctcctactct gtgtgcagga gatcaggtgt ttgtcgacgt atccgtcaga
     9961 aattttgaaa ctgacggtat ctatattttt atttttgggc acactttcca tatcaaacgc
    10021 ctgcaaatgc aagggatgca acttgctgtt atatcagaca atccggccta caaggaatgg
    10081 tttataagtg aatcagctga agaacatcta ttcataatgg ggaaagttct cattcatcag
    10141 tcaatccagt ataacagggt cggataagtc aacatcaccg gcagctgtat tgcgcagctg
    10201 ctctactttc ctgtatctca actcc
//
LOCUS       DAZUCC010000181        10217 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZUCC010000181 DAZUCC010000000
VERSION     DAZUCC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.182"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            89..1483
                     /gene="galP"
                     /locus_tag="ACHY09_003931"
     CDS             89..1483
                     /gene="galP"
                     /locus_tag="ACHY09_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFS3886838.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1560..2057
                     /locus_tag="ACHY09_003932"
     CDS             1560..2057
                     /locus_tag="ACHY09_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFS3886839.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2152..2859
                     /gene="endA"
                     /locus_tag="ACHY09_003933"
     CDS             2152..2859
                     /gene="endA"
                     /locus_tag="ACHY09_003933"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFS3886840.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2939..3670
                     /gene="rsmE"
                     /locus_tag="ACHY09_003934"
     CDS             2939..3670
                     /gene="rsmE"
                     /locus_tag="ACHY09_003934"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS3886841.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3683..4633
                     /gene="gshB"
                     /locus_tag="ACHY09_003935"
     CDS             3683..4633
                     /gene="gshB"
                     /locus_tag="ACHY09_003935"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFS3886842.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4742..5305
                     /locus_tag="ACHY09_003936"
     CDS             4742..5305
                     /locus_tag="ACHY09_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFS3886843.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5305..5721
                     /gene="ruvX"
                     /locus_tag="ACHY09_003937"
     CDS             5305..5721
                     /gene="ruvX"
                     /locus_tag="ACHY09_003937"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS3886844.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5895..6875)
                     /locus_tag="ACHY09_003938"
     CDS             complement(5895..6875)
                     /locus_tag="ACHY09_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFS3886845.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            6893..7597
                     /gene="yggS"
                     /locus_tag="ACHY09_003939"
     CDS             6893..7597
                     /gene="yggS"
                     /locus_tag="ACHY09_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFS3886846.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLDVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7615..8181
                     /gene="yggT"
                     /locus_tag="ACHY09_003940"
     CDS             7615..8181
                     /gene="yggT"
                     /locus_tag="ACHY09_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFS3886847.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8178..8468
                     /gene="yggU"
                     /locus_tag="ACHY09_003941"
     CDS             8178..8468
                     /gene="yggU"
                     /locus_tag="ACHY09_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFS3886848.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8476..9069
                     /locus_tag="ACHY09_003942"
     CDS             8476..9069
                     /locus_tag="ACHY09_003942"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFS3886849.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLIYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9062..10198
                     /gene="hemW"
                     /locus_tag="ACHY09_003943"
     CDS             9062..10198
                     /gene="hemW"
                     /locus_tag="ACHY09_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS3886850.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2388 a         2693 c         2706 g         2430 t
ORIGIN      
        1 ttacactgat gtgatttgct tcacatcttt ttacgtcgta ctcacctatc ttaattcaca
       61 ataaaaaata accatattgg agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa
      121 caaggcaatg acgtttttcg tctgcttcct tgccgctctg gcgggattac tctttggcct
      181 ggatatcggt gtaattgctg gcgcactgcc gtttattgca gatgaattcc agattacttc
      241 gcacacgcaa gaatgggtcg taagctccat gatgttcggt gcggcagtcg gtgcggtggg
      301 cagtggctgg ctctccttta aactcgggcg caaaaagagc ctgatgatcg gcgcaattct
      361 gtttgttgcc ggttcactgt tctctgcggc tgcgccaaac gttgaagtac tgattctttc
      421 ccgcgttctg ctggggttgg cggtgggtgt ggcttcttat accgcaccgc tgtacctctc
      481 tgaaattgcg ccggaaaaaa ttcgcggcag catgatctcg atgtatcagt tgatgatcac
      541 tatcgggatc ctcggtgctt atctttctga taccgccttc agctacaccg gtgcatggcg
      601 ctggatgctg ggtgtgatta tcatcccggc aattttgctg ctgattggtg tcttcttcct
      661 gccagacagt cctcgttggt ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct
      721 gctacgcctg cgtgacacca gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag
      781 tttgcaggtt aaacagagtg gctgggcgct gtttaaagag aacagcaact tccgccgcgc
      841 ggtgttcctt ggcgtactgt tgcaggtaat gcagcaattc accgggatga acgtcatcat
      901 gtattacgcg ccgaaaatct tcgaactggc gggttatacc aacaccaccg agcaaatgtg
      961 ggggacggtg atcgtcggcc tgaccaacgt actcgccacc tttatcgcaa tcggccttgt
     1021 tgaccgctgg ggacgtaaac caacgctaac gctgggcttc ctggtgatgg ctgctggcat
     1081 gggcgtactc ggtacaatga tgcatatcgg tatccactct ccgtcggcgc agtatttcgc
     1141 catcgccatg ctgctgatgt ttattgtcgg ttttgccatg agtgccggtc cgctgatttg
     1201 ggtactgtgc tccgaaattc agccgctgaa aggccgcgat tttggcatca cctgctccac
     1261 cgccaccaac tggattgcca acatgatcgt tggcgcaacg ttcctgacca tgctcaacac
     1321 gctgggtaac gccaacacct tctgggtgta tgcggctctg aacgtactgt ttatcttgct
     1381 gacattgtgg ctggtaccgg aaaccaaaca cgtttcgctg gaacatattg aacgtaatct
     1441 gatgaaaggt cgtaaactgc gcgaaatcgg cgctcacgat taatctcctc aggcttcctc
     1501 ccgtcgcgga ggaagccacc tcttgcagtc atcttttctt cgctctatcc tctgccgcta
     1561 tgaaaacatc ccgtctccct atcgccatcc agcaggccgt tatgcgccgc ctgcgggaaa
     1621 aactcgccca ggccaacctg aagctagggc gtaactaccc ggagccaaaa ctctcttaca
     1681 cccagcgcgg aacctccgcc ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc
     1741 ccgttttgct gttggaaaac agtgaagctt ttattgaaga agtggtaccg cacgaactgg
     1801 cacatttgct ggtatggaaa catttcggcc gcgtagcgcc acatggcaaa gagtggaagt
     1861 ggatgatgga aagcgtgctg ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat
     1921 ccgtgcgtcg caacaccttc ccctaccgct gcaagtgcca ggagcatcag cttaccgtac
     1981 gccgccataa tcgcgtagtt cgtggcgagg ccgtctatcg ctgtgttcac tgcggtgaac
     2041 agctggttgc gaaataacca cctgaactat caggaacttt cctgatctgg ctgattgcat
     2101 accaaaacaa ctttcgctac gttgctggct cgttttaaca cggagtaagt gatgtaccgt
     2161 tatttgtcta ttgctgcggt ggtactgagc gcagcatttt ccggcccggc gttggccgaa
     2221 ggtatcaata gtttttctca ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc
     2281 ggtacgtttt attgcggatg taaaattaac tggcagggca aaaaaggcgt tgttgatctg
     2341 caatcgtgcg gctatcaggt gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa
     2401 cacgtcgttc ccgcctggca attcggtcac cagcgccagt gctggcagga cggtggacgt
     2461 aaaaactgcg ccaaagatcc ggtctatcgc aagatggaaa gcgatatgca taacctgcag
     2521 ccgtcagtcg gtgaggtgaa tggcgatcgc ggcaacttta tgtacagcca gtggaatggc
     2581 ggcgaaggcc agtacggtca atgcgccatg aaggtcgatt tcaaagaaaa agccgccgaa
     2641 ccaccagcgc gtgcacgcgg tgccattgcg cgcacctact tctatatgcg tgaccaatac
     2701 aacctgacac tctctcgcca gcaaacgcaa ctgttcaacg catgggacaa gatgtatccg
     2761 gttaccgact gggagtgcga gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac
     2821 ccgtatgtgc aacgcgcttg ccaggcgcga aagagctaac ctacactagc gggattcttt
     2881 ttgttaaccc ctaccccacg cgtacaaccg cgtggggaga cgacgcggat ttttaactat
     2941 gcgtatcccc cgcatttatc atcctgaacc actgaccagc cattctcaca tcgcgctttg
     3001 cgaagatgcc gccaaccata tcgggcgcgt actgcgtatg gggccggggc aggcgttgca
     3061 attgtttgac ggtagcaacc aggtctttga cgccgaaatt accagcgcca gcaaaaaaag
     3121 cgtggaagtg aaggtactgg aaggccagat cgacgatcgc gaatctccgc tgcatattca
     3181 cctcggtcag gtgatgtcgc gtggtgaaaa aatggaattt acgatccaga aatcgatcga
     3241 actcggtgta agcctcatta cgccactttt ttctgagcgc tgcggcgtta aactggatag
     3301 tgaacgtctg aacaagaagc tccagcagtg gcagaagatt gcaattgctg cctgtgagca
     3361 gtgtggtcgt aaccgggtgc cggaaatccg tccagcgatg gatctggaag cctggtgtgc
     3421 agagcaggat gaaggactga aactgaatct tcacccgcgc gccagtaaca gcatcaatac
     3481 gttgccgtta ccggttgaac gtgtccgcct gctgattggc ccggaaggcg gtttatcggc
     3541 agatgaaatt gccatgactg cccgctatca atttactgat atcctgttgg gacctcgcgt
     3601 tttgcgtaca gagacaactg cgctcaccgc cattaccgcg ctacaagtac gatttggcga
     3661 tttgggctaa cggagaagaa taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa
     3721 catcaacatc aagaaagatt ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta
     3781 cgaacttcac tatatggaga tgggcgatct gtatctgatc aatggcgaag cccgcgccca
     3841 tacccgcacg ctgaacgtga agcagaacta cgaagagtgg ttttcattcg tcggtgaaca
     3901 ggatctgccg ctggccgatc tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac
     3961 cgagtttatc tacgcgacct atattctgga acgtgccgaa gagaaaggga cgctgatcgt
     4021 taacaagccg cagagcctgc gcgactgtaa cgagaaactg tttaccgcct ggttctctga
     4081 cttaacgcca gaaacgctgg ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa
     4141 acacagcgac atcattctta agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt
     4201 gaaagaaggc gatccaaacc tcggcgtgat tgccgaaacc ctgactgagc atggcactcg
     4261 ctactgcatg gcgcaaaatt acctgccagc cattaaagat ggcgacaaac gcgtgctggt
     4321 ggtggatggc gagccggtac cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg
     4381 tggcaatctg gctgccggtg gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa
     4441 aatcgcccgt cagatcgggc cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga
     4501 tatcattggc gaccgtctga ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat
     4561 tgaagcagag tttccggtgt cgatcaccgg aatgttaatg gatgccatcg aagcacgttt
     4621 acagcagcag taacccacct tagcgagaag gatctcgttg agactctgag tgacagcgcc
     4681 cttctttcca cgcatactgg gcgctgttgc ttttttgaac caggaaacag aacctctgac
     4741 aatgaattta cagcatcact ttcttattgc catgcctgct ctccaggatc cgattttccg
     4801 tcgttccgtg gtctacattt gcgaacataa taccaatggt gcaatgggga tcatcgtcaa
     4861 caagccgctg gaaaatctca aaattgaagg gattctggaa aagctgaaga tcacgccgga
     4921 gccgcgtgat gaatcaatcc gtctggataa accggttatg ctcggtggtc cgctggctga
     4981 agatcgcggg tttattttgc atactccgcc ctctaatttt gcttccagca ttcgcatttc
     5041 agacaacacg gtgatgacca cttcccgcga tgtgctggaa acgctcggca ccgataaaca
     5101 accgtctgac gtattggtgg ctctgggcta tgcctcctgg gagaaaggtc aactggaaca
     5161 agaaattctc gataacgcgt ggctaacggc cccggcggat ctgaatattc tgttcaaaac
     5221 accgattgcc gaccgctggc gcgaggcggc aaaactgatt ggtgtggata ttctcaccat
     5281 gcctggtgtg gcagggcacg cctgatgagt ggaaccttac tcgccttcga cttcggtacc
     5341 aaaagcattg gtgtagcggt cggccaacgc attaccggca ccgctcgccc attgcctgca
     5401 attaaagcac aggacggtac gccggactgg aacattatcg agcgtttact gaaagagtgg
     5461 cagcctgacg aaatcatcgt cggtttgccg ctgaatatgg acggcaccga acagccgtta
     5521 accgccagag cgcgtaaatt tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag
     5581 ctccatgacg agcgtcttag caccgtggag gcacgttccg gtctgtttga acagggcggc
     5641 tatcgggcgc tcaacaaagg caaagtcgac tctgcctctg cggttattat tctcgaaagc
     5701 tatttcgagc agggatatta aggcgattta ctagcgcaca gatttctgac aatgtgctcg
     5761 ttgtttcatg ttggatgcgg cgtaaacgcc ttatccaacc tacagtgagt gcgaattcaa
     5821 agttacgctt cccttgtagg cccgataagc ttgcgcatcg ggcatattta caattcgtat
     5881 cagacacagc aacgtcacaa gcgcccttct ttcacccgct gctgataact ctgctgaaac
     5941 gttatcatcc ccacctgctg cccggtttga ataacatgcg gtaactggtg ggttttccct
     6001 tcacgaatca aattccccac cgcgggtgtg ttaatcagca attcaaacaa cgccacgcgt
     6061 ccttcctgtt tatccacttc cagcttttgt gacaacaccg cccgtaaact acctgccagt
     6121 tgattacgca cgggatcttt ttcctgcgcc ggaaatgaat ctaccagtcg ctcaactgcc
     6181 tgcgccgcac cacgcgtatg taatgttgcc agcaccaaat gcccggtttc tgccgccgtc
     6241 agcgccagac ggattgtctc gctgtcacgc agctctccga gcaaaatcac atcaggatct
     6301 tcccgcaatg cagcccgcaa tcccgatgcg aacgtcatac aatgcaaacc gatttcccgc
     6361 tgctggatca aacatcgctg gcttgcatag agatattcga caggatcttc cagcgtcaga
     6421 atatgcgcat cggcatgttg attgagatag ccaaccatcg ccgccagcgt ggtagatttg
     6481 ccgctccccg tcgcccccgt caccagaatc aggccattct cgctcttgag taattccggc
     6541 aataccggtg gcgcaccaag ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat
     6601 gccagagaaa taccttgccg ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc
     6661 agcgacacag caaaatccaa ctgaccattc tccagcaata ttgtccgctg atcgtcatcc
     6721 agccactccc gcagtagctc ttcgacgtcc ggcgcatcaa acggcgcagc ttccattcgc
     6781 ccgcgaatgc gccatcgtgc gggccaggcg ctgcacaggt gtagatccga gacgttatgc
     6841 tttacactaa gggccacaat ttcttccata ttcatactaa gatcctcgga aaatgaacga
     6901 tattgcgcat aacctggcac aggtccggga caaaatctca gcggctgcaa cgcgttgcgg
     6961 ccgttctcca gaagaaatta cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat
     7021 cgcagaagcc attgatgccg ggcagcgtca atttggtgaa aactacgttc aggaaggggt
     7081 agataaaatt cgccactttc aggaactggg cgtaacagga ttagaatggc attttattgg
     7141 cccgttgcag tctaataaaa gccgcctggt ggcagagcat ttcgactggt gtcataccat
     7201 cgaccgtttg cgcattgcta cccgcctcaa cgatcagcgc ccggcagaac ttccccctct
     7261 tgacgtactg attcaaatta atattagtga tgaaaacagt aagtccggga ttcaactggc
     7321 agaactggac gagctggcag ctgcggtcgc tgaactaccg cgtttacgtc tgcgcgggct
     7381 gatggcaatc cctgcgcctg agtcagaata tgtaaggcag tttgaagttg cacgccaaat
     7441 ggctgtagca tttgccggac tgaaaacgcg ctacccgcat atcgacacgc tctctctggg
     7501 aatgtcggac gatatggaag ccgccattgc ggcaggtagc acgatggttc gtatcggcac
     7561 tgcaattttt ggtgcgcgtg attactctaa aaaataagga attaaaggaa cgccatgaat
     7621 acgttgactt tcctgctttc aacggtcatt gagctgtata ccatggtgct gttattacgc
     7681 atctggatgc agtgggctca ttgtgatttt tacaacccct tctcacagtt tgtagtgaag
     7741 gtaacgcagc caattatcgg gccactgcgc cgcgttattc cggcaatggg acccattgac
     7801 agcgcctcgc tgctggttgc ctatattctc agttttatca aagccatcgt gctgtttaaa
     7861 gtggtgacct tcctgccaat catctggatt gccggtttac tgattctgct gaaaaccatc
     7921 ggcctgctga ttttctgggt cctgctggtg atggcgatta tgagctgggt aagccagggt
     7981 cgtagtccga ttgaatacgt gctgattcag cttgccgatc cgctgctgcg cccgattcgc
     8041 cgcctgctac cggcaatggg tgggattgat ttctcgccga tgatcctcgt tctgctgctg
     8101 tatgtcatca atatgggtgt cgcagaagta ttacaggcaa ccggaaacat gctgctgccg
     8161 gggctgtgga tggcgttatg agtgccgtaa cagttaatga tgacggtctg gttttacggc
     8221 tctatattca gccgaaagcc agccgtgatt ctattgtcgg tttacatggc gacgaggtaa
     8281 aagtcgccat taccgcgccg ccggttgacg gccaggccaa cagccatctg gtgaagtttc
     8341 tcggtaagca attccgggtt gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc
     8401 gccacaaaca aattaaaatc attaatccgc aacaaatccc gccagaaatc gcggcgttaa
     8461 ttaattaggt atcctatgca aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt
     8521 gagctggcgt cgctgcttag cgacttcggt cttgatatcg tggcccaaac agacctcggc
     8581 gtagattccg ctgaagaaac cggcctgacc tttatcgaaa acgcgattct gaaagcgcgc
     8641 catgcggcaa aagtgaccgg tttaccggca attgccgacg actccggtct ggcggtagat
     8701 gcgctgggtg gcgcgccggg tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat
     8761 caaaagaacc tgcaaaaact gctggaaaca atgaaagacg taccggacga ccaacgtcag
     8821 gcgcgtttcc actgcgtgct gatatatctg cgtcacgcgg aagatccgac tccgctggtg
     8881 tgccacggta gctggccggg cgtgattact cgtgaaccag cgggtactgg tggctttggt
     8941 tatgatccaa ttttcttcgt tccttctgaa gggaaaaccg ctgccgaact gacccgcgaa
     9001 gaaaagagcg ccatttccca ccgtggtcag gcattgaaac tgctgctgga cgctttacgt
     9061 aatggttaaa ttaccgccgc tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg
     9121 cccgtactgc gatttcaact ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt
     9181 tcagcatctg cttaacgatc tggacaacga tgtggcttac gctcagggcc gtgaagtgca
     9241 gacaatcttt attggcggtg gtacgccgag cctgctttcc ggcccggcga tgcaaacgct
     9301 gctggacggc gtgcgtgcgc gtttgccgct ggcagcggat gcagaaatta cgatggaagc
     9361 gaacccaggc acggtagaag cggatcgctt tgtcgattat cagcgtgctg gtgtgaaccg
     9421 catctctatt ggtgtgcaga gttttagcga agaaaagctg aaacgactcg ggcgtattca
     9481 tggcccgcaa gaagcgaaac gagcggcaaa gctggcgagc ggtttagggt tacgtagttt
     9541 taaccttgat ttgatgcatg ggctgccgga tcaatcactg gaagaggcgc taggcgatct
     9601 gcgccaggct attgaactga atccgccaca tctttcctgg tatcaactga ccatcgaacc
     9661 taatacgctg tttggctcgc gccctcctgt actgccggac gatgacgcgc tgtgggatat
     9721 tttcgaacag gggcatcagt tattaaccgc agcgggctat cagcagtatg aaacatccgc
     9781 ttacgccaaa cccggttatc agtgccagca caatctcaac tactggcgat ttggcgacta
     9841 catcggtatt ggctgcggcg cgcacggcaa agtgaccttc ccggatgggc gcattctgcg
     9901 taccaccaaa acgcgtcatc cgcgtggttt tatgcaggga agatatctgg aaagccagcg
     9961 tgatgtcgaa gctgcagata aaccgtttga gttctttatg aatcgcttcc gtctgcttga
    10021 ggccgcgccg cgcgtggagt ttagtcagta tactggcctt tctgaagaag ttattcgctc
    10081 tcagttagac gaggctatcg ctcagggtta tctcacagaa tgtgcggatt actggcagat
    10141 aaccgagcat ggtaaattat tcttaaactc acttcttgaa ttattcctcg ctgagtaatc
    10201 attaatgcgc ctgcggg
//
LOCUS       DAZUCC010000182        10211 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZUCC010000182 DAZUCC010000000
VERSION     DAZUCC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.183"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            363..1607
                     /locus_tag="ACHY09_003944"
     CDS             363..1607
                     /locus_tag="ACHY09_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFS3886851.1"
                     /translation="MSKKFTKTLLSSSLVVGLLSVSSFTSASEYSYTALDIVSRHGTT
                     YTIVYNSDSDTAYVKWKTEPAGAEGIESGSIVQGLSKINRDGTVEVTTKDGTSTYRVY
                     DREFHDLMNSLVKQKREGSLGKNEGTKTELVSDADIRNLFGRIAAISEMETNVGLDEY
                     GRVATSAESIKERVSLQSYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIA
                     KDVEEQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADGTLTSADGA
                     TKTIAVNDGLVALSGRTDRIDATVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQ
                     IRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTA
                     AKAGVAASDGDVSYNVGVNFEF"
     gene            1694..2167
                     /locus_tag="ACHY09_003945"
     CDS             1694..2167
                     /locus_tag="ACHY09_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886852.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            2538..6530
                     /locus_tag="ACHY09_003946"
     CDS             2538..6530
                     /locus_tag="ACHY09_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFS3886853.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDSSGTVVPAVAGGDIVWNYSKGSGE
                     GTLSQDGKVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRPE
                     GNQTWVGGGIIVNDGHRVDWMVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTVI
                     LAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIAFN
                     RLAAADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMDG
                     GADISGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESR
                     TFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGT
                     SAVGGASGSTTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTSS
                     ALTVSDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGSG
                     EYDFMTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFH
                     TLTAGSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLV
                     TAPAGTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQDA
                     ETPNPVEAVSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVN
                     SSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSD
                     NYTHVQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGAGLYASALFDSG
                     AYIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAELV
                     YGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFD
                     LLANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDN
                     AVNASFRYSF"
     gene            complement(7098..9770)
                     /gene="rcsD"
                     /locus_tag="ACHY09_003947"
     CDS             complement(7098..9770)
                     /gene="rcsD"
                     /locus_tag="ACHY09_003947"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFS3886854.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTTPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLTNQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2574 a         2163 c         2901 g         2573 t
ORIGIN      
        1 taaagaacat gctccgtacg gaagtgcggg gctgatataa agtcacgcag ggaaatattg
       61 tagatgaact gaacagttaa attcggataa tgttgtgata ttgatcagaa attacagtgt
      121 aagcagatgt gaaacagaat gcctgatgag agtaagtttt ttctgatgcc tgttccggtg
      181 gtgtagtcgt ggttctgagt gactacacca agaataatgt aagacaaatc ctaaaacatt
      241 aaattaatat tttggggtta ttttcggaat atcctgaata aagccgcaat taatcatatt
      301 tatttatgag tatgaggtgt agtggctgat ttgtatacag ataatttaac aggatatcca
      361 taatgagtaa aaaatttaca aagacacttc tgtcatcttc tctggttgta ggtctgttga
      421 gtgttagttc ttttacaagt gcaagtgaat atagctacac tgccttggat attgtcagta
      481 gacacggaac tacttacacc attgtttata atagtgattc tgacaccgct tatgttaaat
      541 ggaaaacaga acctgccggt gctgaaggga ttgaaagtgg aagtattgta caaggattat
      601 caaaaattaa tagagatggg actgtagaag ttactaccaa ggatggcact agtacatatc
      661 gtgtgtatga ccgggagttt catgacctaa tgaattcact ggtcaagcag aaaagagaag
      721 gttctcttgg aaagaacgag ggtactaaaa cagagcttgt atctgatgct gatattcgga
      781 atctttttgg cagaattgct gcgatctctg aaatggaaac taatgttggt ctggatgaat
      841 atgggcgagt ggcaactagt gcagagagta ttaaagaacg cgtgtctttg cagagttatc
      901 tggctacaga ggctgcaaat agcgcaattg gcctagataa aaacggtaag ctaactctga
      961 atgagacaga tattgtaacg ccgttacgta tcaatgacgg cgttgtcact atagcgaaag
     1021 atgtggaaga acagaaagaa gggctggcga tccagaagaa aggactgtct gagctttcat
     1081 cccgcacaga tatcattgat gcagcaacgg ctgcgaatac gaataaaatt gattcccgaa
     1141 ccaccgttgg tgtgaatgcg gacggaacct tgaccagcgc tgatggcgct acaaaaacga
     1201 ttgctgttaa tgacggtctc gttgctctgt ccggcagaac agaccgtatt gatgctactg
     1261 ttggtagcgt tgaccgccgg gttactaaaa atacacaggc tattcagtcc aatactcgcc
     1321 agttgcagga acataatgca cgcctgaaca gccagcagcg tcaaattcgt gagaatcacg
     1381 aagagatgaa gcgtgcagca gcacagagtg cagcgctggc ggggcttttc cagccgtaca
     1441 gtgtggggaa attcaacgcc actgctgccc tgggggggta cagtgataaa caggctgttg
     1501 ccgtgggtgt tggttaccgt ttcaatgagc agacagcagc gaaagcaggt gtggcagcga
     1561 gtgatggcga tgtgtcctac aacgtaggtg tgaactttga gttttaattg ctgacagtga
     1621 catgaagcat gacggcaggc gtggagtttt gcctgccgtt ttttctcatg tgagcatgaa
     1681 acaggtgaca gcgatgaaag tgattctggc gacccgcaac cgttatctgg agtatggcct
     1741 gcagcagatg cttgaggggt acaggataat actggcgagg gagtttttta caccagagaa
     1801 caggaagagt gtaccggcac atgatgagtc atgggtgata atctgtgatg ccctccttgg
     1861 caggctgatg tgctgcatgt ttcagggacg ccgttatctt cagattgatg cggaagatgt
     1921 tacggggcgc ctggagacgt acaggaaaat ccggaatggt gagtgggtac ataatacgta
     1981 tgcgcgacct ctgacgatgt ccgagatggt ggtgatgttt ggatacgtgt accgggagtc
     2041 aaagccctgc catcttgccc gtgaaatggg gataaacacg aagacggtga acacctttct
     2101 gtatatggga cttggtaaaa atggtcttaa gtacaggagt gtgaagcacc tggttggacg
     2161 tgcgtagccg gaatgcatat tcgtgagtcg ggtaatgaga gtggaacggt ggcagtgaga
     2221 atggaacaga aaaaaatcag atgcggaaag cggaaataca cagcgggtgc gatgtgcttc
     2281 atgctgtttg tatggctggg gggtgtggtg acattctgtg tgctggccgc tctgataatg
     2341 aggtgaaaac ggaagagagt ttacttcctt aattcgaact ttctgataac tgagaacttg
     2401 ttaaatgcct gtgggtttat gtatttaaat caattggtta atggtgattt tatctttatc
     2461 agtcaaagtt cgttttagta tgatgattgt gcccgctgtt tattgttgtc cgggagaaaa
     2521 tacagggaat ccaggggatg aacaaaatat actcactcag agttaatgac agaaaagaac
     2581 tggttgctgt accggaaacg gtggggggca ggaaaaagtc gtccggcatt cccggtgcgg
     2641 ggatgctttc ccgtcttctt ctggcctcag gagccgttgc cggcgttctg ttttcttctc
     2701 ctgcgctggc atccgttgtc gggaatactc tgccatggca gacctacagg gattttgctg
     2761 aaaacaaagg ggcgtttcat gccggggcca caaatattcc tctttatggt aagaatgggg
     2821 ctgtcagtgg gcggctggac aaggctccga tgatggattt cagtgttgtg gaccagatac
     2881 tgggggtggc aacactgatt tctccgcaat atgtggcggg ggttaagcat aacggcagct
     2941 acaatacggt gcgttttggt tatgcggacg atacgacata tcgccttgtt gaccgtaatg
     3001 agcactggcg tgattttcat actccccggc tgaacaaact ggtgacagaa gttgccccgg
     3061 tgagcgtcac ggatgccgga accggcaagg gggtatacca gaaccgcagt cgttatccgg
     3121 tgttttatcg tatggggagt ggaacgcagt acaccggagc tgcttccggt gcgctgacca
     3181 gaatagcgga tgcttacgtc tggaggactg gcgggacggt gggaagtccg gtcatttctg
     3241 actggagtct ggtgtcaaac ccgggaaatc tttaccaggg tgttaatggt ccgctggcgt
     3301 cttacgggac atcaggagac agtggctcac cgctttttgc ctgggatgcg gtgaagaagc
     3361 agtgggtatt tgttgctgta cttcatggtt atgccggtga gaacgggaag acgaactggt
     3421 ttacggtgat tcctgcaggg gatgtgaata acacgataaa gcaggacagt tcaggcaccg
     3481 tcgtgccggc agttgccggt ggtgacattg tgtggaatta cagtaaggga agcggagagg
     3541 gaaccctttc tcaggatggg aaggtatgga agatgaacgg cttcagggga gggagtctga
     3601 atgacgggaa agatatcacc tttgggggta agggcacggt tgttctgaag gatgatgttg
     3661 tccagggggc gggttcgctc acgtttaatg gtgattatac ggtcagaccg gaagggaatc
     3721 agacctgggt gggcggggga atcatcgtga acgacgggca ccgtgttgac tggatggtga
     3781 acggacttgc gggagacgca cttcataaaa ttggtaaggg aacactggtg gtggccggca
     3841 gtggtgaaaa tcctggcacc ctgaatacgg gggacggaac ggtcattctg gcacagaagg
     3901 ctgatgctgc aggcagggtc cgggcattca gtgaagtcag tattgtcagc ggacgaccag
     3961 ttgtggtgct gcaggacagt catcagattg aaggggaccg cattcgctgg ggataccggg
     4021 gaggtacgct tgatatcaac ggaaatgaca tagccttcaa ccgcctggct gcggctgatg
     4081 agggggcagt actcacaagc cgggcgcgtc ctgccacagt ccgtctggat ttcagccagt
     4141 cagggcagaa ggccgtgatg tggcatggtc attttaccgg taacctcagc gtcctgaaca
     4201 acacgtcatc tgcggtggat tttatcatgg atggcggagc ggatatatcc ggcagtttta
     4261 cacagcaggg cgggggactg tatatccagg ggcatccggt tgttcatgct gtcagcagtg
     4321 aggcggttgc gactgcattg cgaaagcagg gtgataattc tgttctgacg cagccggtat
     4381 catttgctca gaaggactgg gagagcagaa cgttcagcat cggtcaactg aaactgaagg
     4441 gggcggcatt cagcctttcc cgcaatgcga cactgacggg agatattgat gctgataatg
     4501 ccaccatggt gctgggaagt gactcgctat atctggatat gaaggacggc acggggagca
     4561 gcagtgcgcc ggttaagggg acgtctgccg tgggcggtgc atccggtagc actacatttc
     4621 gcgggaatgt gaatatgcgt cattctgccc tgacggtccg cgaccatttt actggcagta
     4681 taacggcatc ggacagccgg atagctgtga gttcagagaa tgtgcgtctg gaagggaatt
     4741 cccgcctgac ctcttctgca cttactgtca gtgacggcgg gcgtttacat gtgaaggggg
     4801 gactggagac agatggtggt gtgacactgg acagaggaac ccttttggtg gacgggggaa
     4861 gtgtcaggaa tgatgtatat gaacgtctgc tggcctggtc agaggagcgt ggcgggctga
     4921 acggaagcgg tgagtatgat tttatgacgg gggcagctgg tctcctgcgg agttatgtca
     4981 gagggagtgc aggtaatgtg aatctgcaga atgcggcctg gatgatgacg ggaaactcat
     5041 ccgtgaagca tcttgagtca tcgggaagtg ctctgtattt cagcaggccg ggcggggagt
     5101 tccataccct gacggcaggc agtatggata tcagcgacag tgttctggtg atgcggacgg
     5161 accttaacca ctcagaccag cttcgggtga cagaatcgct gcggggtaag aataacctgt
     5221 tgttggttga ttttactgag cgttctgacg ggcagaaggc gctgaatatc cctctggtga
     5281 cggcgccggc cggtaccggt gcggatgtgt tcagtgtgaa aacgcgtgat accggcttca
     5341 gtcatatcac acctgttgtc agggctgaac aggggacggg cggcacggcc tggcagctga
     5401 atgttgtgca gccggaaact gccgcagagc ctgtggcgac gcggcaggat gcagagacgc
     5461 cgaatcctgt ggaagctgta tcaccgctcc cttcaccggt ttctgctccg tcatcggctc
     5521 cggaggcgtc agtgacggat gtactgaccg gagagaatgc gggggaatca ggtgagtacc
     5581 gtgatgagac ccggtggctg ctgacaggat accggagtac ggttaatagc tccgcggtca
     5641 gggatgcggg gatgctgatg tcaatggggc accgtaactt cattaatgag gtcaacaacc
     5701 tgaacaaacg tatgggtgac ctgcgggaca tcaacggcga ggccggtgca tgggcccgta
     5761 tcatgagcgg aaccggctct gccggcggtg gcttcagtga caactacaca cacgttcagg
     5821 tgggggcgga taaaaaacac gaactggacg gtctggacct cttcaccggg gtgaccatga
     5881 catataccga cagcaacgcc ggcagcgaca ccttcagtgg taaaacgaag tcagtgggtg
     5941 cgggtctgta tgcttcagcc ctgtttgatt ccggggccta tatcgacctg attggtaagt
     6001 atgttcacca tgataatgag tatacggcgt cctttgccgg actcggcaca aaagactaca
     6061 gctctcattc ctggtatgcc ggtgctgaag tgggttaccg ctatcatgtc acagaggatg
     6121 cctggattga gccacaggcg gaactggtct atggtgctgt ctccgggaaa cagttcagct
     6181 ggaaggacca gggaatgaac ctcaccatga aggataagga ctttaatccg ctgattggcc
     6241 gtaccggtgt ggatgtgggt aaatctttct ccggtaagga ctggaaagtg acggcacgtg
     6301 ctggtcttgg ctatcagttt gacctgctgg cgaacggaga aacggtactg cgtgatgcat
     6361 ccggagagaa gcgtattaag ggcgagaaag acggccgtat gctgatgtct gtgggtctga
     6421 acgcagaggt ccgtgacaac atccgttttg gtctggagtt tgagaaatca gcctttggta
     6481 agtacaacgt tgataatgct gtcaacgcca gcttccgtta ttcattctga cggttgttac
     6541 tgaaggggat tatggcctga cggctccctg taaataaaga caggcacaga agattgtgag
     6601 ttactcagtg ttccaccaaa aagtaaggtc gcaactctgt gcctgcttcg taagtgtgca
     6661 gtgagggcca tatggttata actgaagcat ccgacgacgt agtgtctacc agagtgtcag
     6721 atatgcagga aaatatgagt tccgacagaa atgcagtaaa aaaaggagtc tcgcactccc
     6781 ctccataaaa ctccagggct gtaagttgta ttaaaaagct gatttttctt tagcgataca
     6841 gacctcaata agtataacac ccttcagtta ctgtggttat gcacaggtgt tatttttaac
     6901 agtgtggata aacagtgtcg attattcaga ttggttatgg ggcatgtatg ggacattttt
     6961 aatcggcgaa atacgtcgaa gttcgtcgat atggtaaacg aatcatctat ccaacccttg
     7021 aaaaacggcg ctcctggacg atcttcgtcg atttttaaaa tgcaacaagt acgttgttca
     7081 tgtaataggc taccttgcta cagcaagctc ttgacataac tgtcaatgtc gctgatgtat
     7141 ttttctattc ctggaacatc cttctcacga atcagatgtt ccagcgtttc acataactgc
     7201 ttgccgggta ccagatttag catggcaaat acgcctttaa gacgatgagc cgtttgggct
     7261 aacgcagcaa agtcactggt cgctgcttca gtatacagcc tcttaacatc atccggtact
     7321 gtgtctacaa agagcgcata atagccgctg gcatggagtt gcgcattttc atctccgccc
     7381 agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt caattaattg taagaccgct
     7441 tcctgcatag cgttgctcat attgaagttg acgcacaatt gaccaggccc aatttcccgt
     7501 acgccagact catcatcgct taaaagcaag ccagaggcag taagattaga cggattatcc
     7561 gttaaaaaga tatcataatc ttgactaatt aatctttcat cgggtgtgat acaggttgca
     7621 ccccaatttt ctaactggcg agtgacaata ttccgaattt ctgccgaagt aacatccacc
     7681 attacgcaga catcatccag taaacgctct tcttcctctt caacttccgg gtcagctgcg
     7741 agcattttga tatgcacaga gtagcgagta ccaagcccat cccgcgtttt gatgtttaaa
     7801 tgaccgccca gtttacgcgc cagttgatcg ctcagccaga atgccagcgg gtccgccttg
     7861 ccatagcgat cgttttgggt ctggttgata aacgggaagt gcaaattatc catttcatga
     7921 atgcttacgc cttctccagt gtccagaata cggaacgtca ggcggtcttc ggaggactca
     7981 tcctgatcaa cctcaagggt gatttttccc aattgcgttg aggtcacggc atattgcatc
     8041 agtaacagca aaatacgtcg taaggcatcg cgatcgccgc ggcgcatgtc gtgtgctttc
     8101 agatgattgt taatcagcag ttgcagacct ttacgcttga tggcaggcaa cactgaaggc
     8161 acaacttcat caattaaatc ctgcacggag aacagcaccg tctcactttt ccagctatcg
     8221 tccgcaagca tgttcgctaa ctgtatttca tcgaccagcc gcaccagcac atccgcctga
     8281 ttcgtcagtt gtttgccttc cggggcgttg agtttagccg cgctctccgc cagggactgt
     8341 gcgggttctt tcagcgcatc gccaatgttt ttcataaagg tcatccgccc ctgctggttt
     8401 ttctcataca gacgctgcgc ctgcttgagt ttcttgttta ccagcacttc gcgatcctga
     8461 tcgcgaataa tgaaaatttg tgtgcgcggc gcaacctggc tgcggaacat gcggatctca
     8521 tacagctcgt tattgatcgt cgcctgaata atcccctgat gctgttccgc catggtggtg
     8581 atgttttgca gattcaaatg cggcagcaaa tgatcggcaa ttttgttact tatgacagtg
     8641 cggttcgatt cctgatcgtg aaccagcagg ccgagcggca gcagtgagac tatctcttca
     8701 ttgattgccc gtaaaatgcg caattcgtta ttgaccgccg tgttgggtag actttctgta
     8761 ctgcgactgg agaaatggcg aaatgtggta tagccaaata acgccagcgc cagcaaaccg
     8821 atgttcagca gcagtggcag cagaatgttt tgcaacgtat ccagcaataa ggtgccataa
     8881 ggaacctgcc agaccaggcg catatcggta gagttgagtg ccgaggagat ttcaatcttc
     8941 gtactgttaa agtggatact gacgctatcc gtcccttctt tctcattatc attgtctccc
     9001 gttgccgtcg cgtctggctc aaggcggaaa ctgtccagcg gcatacccgg tgggatcaaa
     9061 tcattaatcg gcagatcaaa agccacaacc gttgccagat gtcccggctg gttgaacgtg
     9121 gtacgcaagg taaagtaatg accgttctgc caggccaggc ggcgcatgtt agaaaagctt
     9181 tcgcgttcat cgagggcgtt ggcctgttgc aacatctctg cacgacgtga atcaacaatg
     9241 tcgctgacgg tcgattcttt aaatccggag gtgagatctt tgaggggcag ggttgagatc
     9301 agcaccagac tgttatcctg accattcagg taatacatcg accacggtac attttctgcg
     9361 ccccacaatg tatccagata agtggacatc cgttgagtca tctcaagcgt tgagttgtcg
     9421 tgagagccaa agattaacgc ttccgttttg cggcgtggtt tctccagata gtagacatcc
     9481 tgtttcaggc gcgtctcttg taaaccttcg ccggaggagg gggtggtcgt cgcggcaatg
     9541 ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga cgcgcttttg gatagcgtgg
     9601 gtaatgtcga caatctggta acttttatct ttcagccagg cgttaacggc gctttgtacc
     9661 attacaccca tcgtcaccag taacacaatg atcaacagta aaaagaagcg ggtaatgctc
     9721 cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt tctgacgcat tcgtgtttat
     9781 gacctgttaa aacttcgcga agtgataggg cagtataaag ggtacagtgt gaaattccag
     9841 actcttaccg gattgatgat tctctattct cccgtgatat ttatcatcct gcattggggg
     9901 aaatagatgt acaagcgcca ttttgctgta caaataacct acaaaaagcc taacgaaatt
     9961 aattgcaaat aaacgaagat tgctggaaat tatgctgatg ttaattattt gtgaaatagt
    10021 taacaagcgt tatagttttt ctgtggtagc acagaataat gaaaagtgtg taaagaaggg
    10081 taaaaaaaac cggatgcgag gcatccggtt gaaatagggg taaacagaca ttcagaaatg
    10141 aatgacggta ataaataaag ttaatgatga tagcggaaga tattctagtt gcgagtgaag
    10201 gttttgtttt g
//
LOCUS       DAZUCC010000183        10013 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZUCC010000183 DAZUCC010000000
VERSION     DAZUCC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.184"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..135)
                     /locus_tag="ACHY09_003948"
     CDS             complement(<1..135)
                     /locus_tag="ACHY09_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886855.1"
                     /translation="MLQSSADKACNVLVKKVRYLNDVRKITKMMNGKLIYSWLVWPCS
                     E"
     gene            213..1307
                     /gene="ddlA"
                     /locus_tag="ACHY09_003949"
     CDS             213..1307
                     /gene="ddlA"
                     /locus_tag="ACHY09_003949"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS3886856.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1510..1722)
                     /gene="yaiZ"
                     /locus_tag="ACHY09_003950"
     CDS             complement(1510..1722)
                     /gene="yaiZ"
                     /locus_tag="ACHY09_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFS3886857.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1982..2290
                     /gene="yaiY"
                     /locus_tag="ACHY09_003951"
     CDS             1982..2290
                     /gene="yaiY"
                     /locus_tag="ACHY09_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFS3886858.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2349..3443)
                     /gene="yaiW"
                     /locus_tag="ACHY09_003952"
     CDS             complement(2349..3443)
                     /gene="yaiW"
                     /locus_tag="ACHY09_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFS3886859.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3456..4676)
                     /gene="sbmA"
                     /locus_tag="ACHY09_003953"
                     /pseudo
     CDS             complement(3456..4676)
                     /gene="sbmA"
                     /locus_tag="ACHY09_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            5028..6185
                     /gene="ampH"
                     /locus_tag="ACHY09_003954"
     CDS             5028..6185
                     /gene="ampH"
                     /locus_tag="ACHY09_003954"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFS3886860.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRVQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6186..6809)
                     /gene="iprA"
                     /locus_tag="ACHY09_003955"
     CDS             complement(6186..6809)
                     /gene="iprA"
                     /locus_tag="ACHY09_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFS3886861.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGSCAGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6895..9843)
                     /gene="ehaB"
                     /locus_tag="ACHY09_003956"
     CDS             complement(6895..9843)
                     /gene="ehaB"
                     /locus_tag="ACHY09_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFS3886862.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWADEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
BASE COUNT         2571 a         2497 c         2389 g         2556 t
ORIGIN      
        1 ctcactgcat ggccatacaa gccaggaata aattagcttc ccgttcatca tttttgtgat
       61 cttgcgcaca tcattgagat aacgcacttt cttcaccagt acgttgcacg ctttgtcagc
      121 agaagattgc aacattctgt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat
      181 gcactttgtg ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt
      241 ttggtggtaa atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg
      301 ccattgataa aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa gggcaatggc
      361 acgtcagcga tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc
      421 gcccttcggc gaccagcctt gcgcaagtgc caggtaaaca tgagcatcag cttatcgacg
      481 cgcaaaacgg tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc
      541 tgggcgaaga tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt
      601 ctgatgttct ggcttcagca gcctgtatgg acaaagatgt caccaaacgt ctgctgcgcg
      661 atgccgggct gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca
      721 gttttgccga agtggagtct aaactggggt taccgctgtt tgtaaaaccg gctaatcagg
      781 gctcttctgt tggtgtcagc aaagtaacca gcgaagaaca gtacgcaatt gccgtcgatc
      841 tggcgttcga gttcgatcat aaagtgatcg ttgagcaagg gataaaaggt cgtgagatcg
      901 aatgcgcagt tctgggcaac gacaatccgc aagccagcac ctgtggcgag atcgtactca
      961 ccagcgattt ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag
     1021 ttccggcagc cattgcgcca gaaatcaacg ataagatccg ggcgattgcc gttcaggctt
     1081 atcagacgtt gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg
     1141 aagtggtgat caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga
     1201 agctgtggca agccagcggt ctgggttaca ccgacctgat cacgcgtttg attgagctgg
     1261 cgctggagcg tcacgctgcg gataacgcat tgaaaaccac aatgtaattt ttaaatgcct
     1321 gataactttt attgggcatt taagactgat ggcaaacgta agattgcctg atgcgctttg
     1381 cttatcaggc ctaccagata attgcaatat actgattttg catgattttg taggcagggt
     1441 aaggcgttcg cgccgcatcc ggcaaaaaca acgagcactt tgtcagcccc cagaacgccc
     1501 aatcctctgt tattgggcgt tttcagtttc ttcatcgcga cgacgaataa tcagccccgc
     1561 cagccagaaa ctaatcaccc acgtcaccag accgacggca taggtctgcc aaccttttgc
     1621 ttcaaatccc agtaaaccca ccacgccatt aagaatgaat ataaggccaa tggcgaacgc
     1681 atagtagtgc cagtcacggc ggatttttac aggcaagttc atggcagctc cgttaacgaa
     1741 aaacaacatc cttgcatatt ctgcaacatc cgtctgtggc gtagacaaca tttccgtaat
     1801 tgttaataaa tttaacttaa ggttgtcatt ttgtgactca cgacgaagat tcacgcttcg
     1861 ggaatattcc caacatcaat ttaccatgat tctgatattg acaatcttaa tgatctgtca
     1921 gacttgccat aagccgctgg tatcacacca gaagagaagc agatgacttc cggaggtttt
     1981 tatggctgat ttcaccctgt caaaatcgct gtttagcgga aaatatcgca atgcctcttc
     2041 aacgcctggc aacattgcct atgcgttgtt tgtgctgttt tgcttttggg ctggggcgca
     2101 attgctgaac ctgttagtgc atgcgcccgg cgtctatgag cgcttaatgc aggtccagga
     2161 aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc accattttcg ggctgatccc
     2221 gtttttagtt ggctgcctca tttttgcagt agtggcgcta tggctgcact ggcgacatcg
     2281 ccgccagtaa gatttacgtt gccagatgcg gcctaaaacg ccttatctgg catgacattc
     2341 ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt cgcaaaccag
     2401 gccgtagtca ggttgcgggt gattttcggg ctttccagtt gaatgccagg caacatttcg
     2461 cggggtagtg atttacccgt tttcgcttcg gcaagttgat aaactttctt gtacagcgcc
     2521 gtctcctcaa aagagaagct gtcccctttc tctaactggc ggcgaatttc actgtcgctc
     2581 atccccagtt ttcccgccag tttgcgcgtt gccagttcgg ttttcccggg ctctttactg
     2641 tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt actgaccgcg
     2701 ttctgaaacg cggcattacg gctggcgtac cagccagcgt taaaatcagc aaaacggtat
     2761 atcggcgcgc tatagctggc gggataattc agtaaatggt aagtaccaaa ccacaacccg
     2821 ccgcgacgac tgaagacttc ctgacggact gtaccgtcca ttttccacgg ataccctttg
     2881 gtatgctgtt cggcaaaggc aatgctgacc tgcatcggcc caccggtgcg caccggatta
     2941 agcgaaccaa acaacgtctg ccccattggc accatgctga tcagatcgtc aaaaatcgcg
     3001 ctcaattgct tttcagtacg caccgaatct aaccgttcgc tatagctttt gccgtttggc
     3061 gatttgattt ttagtgcggt atgcaccaga aaagcaggaa tgtgcatccg ttcagcacga
     3121 cggtcaatct cttgccaggc gatcttgctt aagcccggaa cagccggatc tgcctgataa
     3181 tttgactcct gctgcgccac cgccagcacc gagcagacgt tttccagcgt cggtgccagc
     3241 ccctgacttt caaacgtggt ggcgagatct ttcgcccagg catcgcggtc tttcacgctc
     3301 gcaggcattt tctgccgcac gacgctcgcc acatctatcg ctttttcgcc ctttttcaac
     3361 ggttgcggag cctgactact acatcccgct aaaaccagaa ccgccaacaa tgacagcgaa
     3421 gatagatgat tgacgcgcga cattacttct cctttttagc tcaaggtatg ggttacttcc
     3481 tgaattttgt caccatccag ctcatgttca aagctgcgca gacgtttgta gatagacatc
     3541 aactcaacca gtgtggtcca tgagttaatc aggtactgga aagcaccgcg aacctgacca
     3601 aaaacgttag taatctgcgt catcaggccg agtgtaatcg taccggcaac aattgacgga
     3661 aacagcaaga acaaaccgaa aacgttatcg acctgcaaat agagaatgcg ggcgatgttg
     3721 aaatacatat agtgaaaata gaggcggaaa tagtttttcc gtacggcgct aaacagctcg
     3781 cgtaccgtag gcggcgtcgc acgcgtggca tcgtcttcac cataaaccag ctctttacgg
     3841 taggcagcct ctactcgctg gtttttaaac tccagccccg gcagtttgat ccctaccact
     3901 gccagtaatc cggtccccat cagcgaccag acgatagcgg caatcaccag accatacgga
     3961 atgtgcccga caatcggcag ctccggcaca tgtgcggaga gcgttaccag caccggcagg
     4021 aaggcgatca acgtcatgat agcgttgata aagctgacgc ccatattctc cagcgttgaa
     4081 gcaaaacgca tggtgtcttc ctgcacacgc tgcgcggccc cttcgatatg acgcagttgt
     4141 tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc agcggaacac gtagtgactg
     4201 acaaagaagt tgttcagcac actgatcacc accgcgatca gcgcaatccc cagaaagact
     4261 cccacttcgc ggtaaaattg ttcgatggtg actttatgcg gcgaacttag cgccgtttga
     4321 atcagatcat agaacggcgc ataccaggcg ttgacggcaa ccccgacttc caccaaaaac
     4381 caggtgacga agataatcag cgcagtaccg agtattgacc agtattgcca acggtgcggg
     4441 ctatagataa accagaacaa tgcaaaaagt cctacgcaaa caatgtagta agcgtaaaaa
     4501 atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc ggaagcgccg
     4561 gtgatacgcg ccacccaatc ccccccaccg gcttaccaga agataacggc aatcaatgcc
     4621 caaacaaagg ccgagagaaa aaacgttccc ggctttggga aaaaagactt aaacatatgc
     4681 actcctgcta acttcttatc gttttaacaa ctgctgtgta ttaaccgcat gaccgctaca
     4741 aagcgtcagc caaatgaaac cgcccattgt gacccatagc aaggcgctta gttcgccagc
     4801 ctgagcataa gaattgtttc gacggatttc acaaaccaaa gattgtttac ataagcggat
     4861 taacacgcgc aggatcacaa atgcaggcat tggtgagcag gaaaaagatc gtgtttacgc
     4921 gccgttacgt ttttaacact attattgcac taattcgccc tttgcaatcc atcaatgagt
     4981 agtataaata cgctcagtta ccttcattca atctatggac accaccgttg aaacgtagtc
     5041 tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc cgtccatgcg gcgcagccga
     5101 tcactgaacc ggagttcgcc tctgatattg tcgatcgtta tgccgatcat attttttacg
     5161 gcagcggtgc cacggggatg gcgctggttg ttatcgacgg taaccagcgc gtctttcgca
     5221 gttatggcga aacgcgacct ggtaataacg ttcgcccgca gctggattcg gtcatacgta
     5281 ttgcttccct caccaagcta atgaccagtg agatgctggt gaaattgctc gaccagggga
     5341 ccgtgaagtt aaacgatccg ctaagtaaat atgctccgcc aggcgcacgc gtaccgactt
     5401 acaatgggac gcccattacg ctggtgaatc tggcaaccca taccagcgcc ctgccccgtg
     5461 aacagcccgg tggcgcggca catcgtccgg tatttgtctg gccaacgcgc gagcaacgct
     5521 ggaaatacct ttctacggcg aagctgaaag ccgcgccagg cagtcaggcg gcgtactcta
     5581 acctcgcatt cgacctgctg gcagatgcac tggcgaatgc gtccggcaag ccttataccc
     5641 agttgtttga agagcagatt acccgtccat tggggatgaa agacaccacc tacaccccct
     5701 caccggatca gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg tgcaataaca
     5761 cgctggcggc aattggcagc ggcggcgtct attccacgcc aggcgatatg atgcgctgga
     5821 tgcagcagta tttgtcatct gatttttatc agcgcagtaa ccaggctgat cgcatgcaga
     5881 cgttgattta tcagcgcgtg cagtttacaa aagtgattgg catggatgtc cccggcaaag
     5941 cggatgcgct tggtttaggc tgggtatata tggccccgaa agagggtcgt ccagggatta
     6001 ttcagaagac aggtggtggt ggcggtttca ttacctatat ggcgatgatc ccgcagaaga
     6061 acattggcgc atttgtggtg gtcactcgtt cgccattgac gcgcttcaaa aatatgagcg
     6121 atggcatcaa tgacctggtg accgagctaa gcgggaataa accgttggtt atccccgcat
     6181 cctgattaat actctgaagg caaacggttg atagcgacca gtttgccttt attcatttcg
     6241 atatagccgc ctttacgcaa agcagcgaga acttccgcaa cgacagaacg cgatatacgt
     6301 gtacgttgat ggatataatt catcacacca atacgtgatc gcaattcttc attccagtca
     6361 atcattgata ataacgtcgc gcggatttgt tcgtaggaat tatgcccaat gagctgaacg
     6421 tcgcgtaatt ccagaattct attttgccag gctaaccagt aaaaagcgtc tcgccagagt
     6481 tgattttgtt caataagagt aatggtttgt ttggctggta aatgatatcc cgcacagctt
     6541 ccttctgata ttaatttgta tggtatatcg tttttcatta aaccatcagc cagccccata
     6601 atataaggag cctgggtaat accgataagt acgttttctt ctctacgcag agagataacg
     6661 ccctctaaaa taacgaaagt atcttcgcta ttgacatcac tggaaaatat aacttgcttt
     6721 tcattattaa actcgaagcg cgtaccgtat ctggacaaac atttatcgag cttaccaaat
     6781 tcctgaagag gtttaactac agataacatt tgtgcgtcct ttgcagtaat gcccgtcaaa
     6841 tccttgacgg gcattattta gattaaatta cctatttctt cggagtgaag aatattacca
     6901 ggtatattta acacccacat tcgcggacca gtcttgatct acgtcaccac caccgaggta
     6961 gttagcatcg gtataggcgc tgaagttctt ggtgaagctg aactgagtac ccagaccaac
     7021 acgtaccgca gacccttcag taccgttatc gatggaatca ccgttcacat cgttatcgtt
     7081 gttagagtcg tcgtagacgt aagccagttt gaagtacgga gtcagagcct ggtcttcgct
     7141 gtaggtgaag gtataacctg catctacacc cagttcataa cgcatgctgt cgtaagactg
     7201 accatcaact ttcatgtcgt tgctcagctg gtaatcatca ccagactgga acagaccaga
     7261 aacgctgccg taaggagtca cgtaacctgc atcacccagt ttgaagtcgt aaccggcttt
     7321 caaaccaaag ccccaagcgt cggagttggt gctgccgtca acgtaagtac cgttgctcat
     7381 ggttgcagac aggtcgttgt tgaagtgaga gtagctcaag ctaccgtcaa caaagacgtt
     7441 gttcgcgaag tgagcagaag agtagatgta ggcagtctgg ctgtcttgat ccacctgacc
     7501 agaacggtca ttcatatcac ctttagcgaa gcctgcagcc gcaccgacga tccacttagc
     7561 gttgttaccg tcaattttgg tatcaacacc gaccatgatg ccgttaacat cctgatcata
     7621 gttgatggtg ccgttgtcgc cgttgaagtt accaccgaag tagcttaccc atgcgccgcc
     7681 gttatcagcc agaccatgac gagagttggt cagacgagta ccaacggtgt cttgttccag
     7741 gttccagata ttggtgttcg cagacgggat gctcagcgcc atgttagcgt agtcggtcag
     7801 ctccatctgt tgcagaacaa cggtgttacc acgctgctca gcctgatagg tgtatgcacc
     7861 caggtcagct ttgttagcag cagagaaggt cgcgttgctg tttttgtcgt tgacgtagat
     7921 cagctcgttg ccgttgtagt cagcgataga accagcacca gtagcgttgt cgatacgaac
     7981 tttgtagtta ccggttgcag ctgcaacatg gtcaccgttg ttatcaactt cgttctgacc
     8041 agcttcgata gaagccgtgt tgtcgttatc gccgttaccg ttaatggtca ggtgaccatc
     8101 agagttcatt gcgattaagc cataaccgta gtttgacttg gtagtatcgt tggtacggtc
     8161 gttggtcaga tcggcatcga gtacgaagcg gttgctttcg atgttgaaca cacctgcgtg
     8221 aatgttacca ctggtatcag tagtgctggt caggttcaga gtatcaacct gcagagcatt
     8281 cgccgcagtg ttacctacat ccaggatacc gtcgttagtc actttgatgt tgttagcata
     8341 cagagcagca tcacctacag cccagccagc agaaacttca tcagaaatgg ttacggtact
     8401 gttgtcgaca gtcagtgaat cagttgcaac atgaccatct tcgttgatgt tcagcgcaga
     8461 aagaccagtc agagagatag agtcagaaat cagcgttgaa tcagcaacat taacagttga
     8521 accactgttg atgctcagag tatcaatcag agaagttttg gtggtatccc actgtgaacc
     8581 ggtgttcagg gtcacattga acagaccgct ctggtaaact tcgttaccga tgatgtgatc
     8641 aaattcgctg tattcagagt cgctgttttc tacaccaaca gttgatgaag gccacaggct
     8701 gttagctgcg atgtcgatca gagttgcagt ggcttcagta ttgattccat atgtatagga
     8761 gtcatttaca ccatcgtcat cagtatcaac accaatctga tgcgcagaca ttgccgcacc
     8821 gacccacttg ctgccattgt tcagggtcag atccaggcga tcagtaccat cccaaccgtt
     8881 agtatctaca tcaccgtcag catcacgata gctatcacga ccttgtggga agaagttctc
     8941 atcaaaatta cttgagaaga atacatcgcc catcagtgtg gaattgtcga atgttgcagt
     9001 ggtttgcatg gcgttatcag catatggatg tgcaataaca gctaatgcaa catcatcagc
     9061 attccaccca tcaccattgt aatcactagc attaccagta ttaccaaacc aaccagtagt
     9121 accttcatca gcccatgaac cagaggtcac ggtagaatct ttaacagtga tggtgttatt
     9181 aaatacttcg ctgctgttta caccagtgct gacagtgttg tcgtcgatat cttcccactc
     9241 ataaccctga gtcagcgtaa tacctgctac atgagagtta ttagagatgg acagatcgac
     9301 ttcctgatcc agagtgatgg cagtaccaat gttaaataca tttacaacat gagtgtcagc
     9361 ggcattattg taagtgccgt tatatgtgta gtgttcgtag ttgtcatcga tcgttgaatt
     9421 atcaacggtc agcgccagac gatcatggta gtagtcatcc gcacgatcag cacaatcagt
     9481 tgtcatgcac tcggaataaa tcataccgtg aatagtgctg tttttgattg tcaggctatt
     9541 ggcgttggta ttagtagaaa taccatcgtc cagatagtag gtagaaatca cgccgttaac
     9601 ggtagcgtta ttaatgaccg gatagatatc gccgttgtaa taactatcag cggcatagtt
     9661 attccagcct acataaccat catagtaaac gtcatcagca taggtggcgt cgttgtagtg
     9721 atggaaagta ttgtaagttt gaccagaaat atcgttggtc gctgcattag cctgagaggt
     9781 gatagccaga gtgcaagcca atgctaattg tgatactaca agtttctttt tccaggagtg
     9841 catttgtcat ccctcctcag ggacgtaata agttgatcca tcaattatca atgcatagaa
     9901 aacatattat gtttcgcgtt tacgattatg cggcgttata gaggaatggt tcaattattc
     9961 tttcccctaa gtccgattgc ggacatatat aaagcacgtg aacacacaca aag
//
LOCUS       DAZUCC010000184         9995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZUCC010000184 DAZUCC010000000
VERSION     DAZUCC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.185"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(164..239)
                     /locus_tag="ACHY09_003957"
     tRNA            complement(164..239)
                     /locus_tag="ACHY09_003957"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Lys,seq:ttt)
     gene            complement(243..318)
                     /locus_tag="ACHY09_003958"
     tRNA            complement(243..318)
                     /locus_tag="ACHY09_003958"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(283..285),aa:Val,seq:tac)
     gene            complement(370..445)
                     /locus_tag="ACHY09_003959"
     tRNA            complement(370..445)
                     /locus_tag="ACHY09_003959"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Lys,seq:ttt)
     gene            complement(448..523)
                     /locus_tag="ACHY09_003960"
     tRNA            complement(448..523)
                     /locus_tag="ACHY09_003960"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(488..490),aa:Val,seq:tac)
     gene            complement(659..734)
                     /locus_tag="ACHY09_003961"
     tRNA            complement(659..734)
                     /locus_tag="ACHY09_003961"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(699..701),aa:Lys,seq:ttt)
     gene            complement(899..1690)
                     /gene="cpoB"
                     /locus_tag="ACHY09_003962"
     CDS             complement(899..1690)
                     /gene="cpoB"
                     /locus_tag="ACHY09_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFS3886863.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     EAIVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1700..2221)
                     /gene="pal"
                     /locus_tag="ACHY09_003963"
     CDS             complement(1700..2221)
                     /gene="pal"
                     /locus_tag="ACHY09_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFS3886864.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2256..3548)
                     /gene="tolB"
                     /locus_tag="ACHY09_003964"
     CDS             complement(2256..3548)
                     /gene="tolB"
                     /locus_tag="ACHY09_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFS3886865.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3681..4946)
                     /gene="tolA"
                     /locus_tag="ACHY09_003965"
     CDS             complement(3681..4946)
                     /gene="tolA"
                     /locus_tag="ACHY09_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFS3886866.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(5011..5439)
                     /gene="tolR"
                     /locus_tag="ACHY09_003966"
     CDS             complement(5011..5439)
                     /gene="tolR"
                     /locus_tag="ACHY09_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFS3886867.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5443..6135)
                     /gene="tolQ"
                     /locus_tag="ACHY09_003967"
     CDS             complement(5443..6135)
                     /gene="tolQ"
                     /locus_tag="ACHY09_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFS3886868.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6132..6536)
                     /gene="ybgC"
                     /locus_tag="ACHY09_003968"
     CDS             complement(6132..6536)
                     /gene="ybgC"
                     /locus_tag="ACHY09_003968"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFS3886869.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6686..6979)
                     /gene="ybgE"
                     /locus_tag="ACHY09_003969"
     CDS             complement(6686..6979)
                     /gene="ybgE"
                     /locus_tag="ACHY09_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFS3886870.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6979..7092)
                     /gene="cydX"
                     /locus_tag="ACHY09_003970"
     CDS             complement(6979..7092)
                     /gene="cydX"
                     /locus_tag="ACHY09_003970"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFS3886871.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(7107..8246)
                     /gene="cydB"
                     /locus_tag="ACHY09_003971"
     CDS             complement(7107..8246)
                     /gene="cydB"
                     /locus_tag="ACHY09_003971"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFS3886872.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFHLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8262..9830)
                     /gene="cydA"
                     /locus_tag="ACHY09_003972"
     CDS             complement(8262..9830)
                     /gene="cydA"
                     /locus_tag="ACHY09_003972"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFS3886873.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2434 a         2734 c         2400 g         2427 t
ORIGIN      
        1 aactgagcta acgacccata cgggtgttac ctgaaagatt tctactcgga accaatcttt
       61 aaaattggtg ggtcgtgcag gatgactcgc ttcgctcgcc ctacgggccg ttgctggcgc
      121 aacgttatcc ttcacgctta acatctgagt ttgatgttaa aactggtggg tcgtgcagga
      181 ttcgaacctg cgaccaattg attaaaagtc aactgctcta ccaactgagc taacgacccg
      241 agtggtgggt gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc
      301 cagctgagct aatcacccga tactgcgctg aatactacaa actacttcca ccgaacccac
      361 cgtaattagt ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact
      421 gctctaccaa ctgagctaac gacccggtgg tgggtgatga cgggatcgaa ccgccgaccc
      481 cctccttgta agggaggtgc tctcccagct gagctaatca cccgatacta cgctggatac
      541 tacaactaac taggttagtg gtgggtcgtg caggatgact cgacttcgtc tcgcccttcg
      601 ggccgttgct gacgcaacgt tatccttcac gttttactac cgcttccacc ttagcgattg
      661 gtgggtcgtg caggattcga acctgcgacc aattgattaa aagtcaactg ctctaccaac
      721 tgagctaacg acccactttt tcgttgcttt cggtttgttt gatatcccgt ggcaacggcg
      781 gcatatatta ctgatttcag acttgagcgc aacaaaaatt tcgatgcaga tcactcaact
      841 gcttatgatt cgcacgacac gaccagaaat aatgcgactt ctggtcgtgt gttatgcatt
      901 acatcgcgtt cagacgtttt tgcgcctgtt tagcgccatc agtaccaggg tatttactga
      961 taacctgctg gtatacggct ttcgcttttg cggtgtcacc tttgtcctgc atgatgacgc
     1021 cgactttaaa catcgcatct gcagccttcg gtgacttcgg atagtttttc actaccgaag
     1081 caaaataata cgccgcatca tcttttttac ccttgttgta gtttaactga cccagccaat
     1141 aattggcgtt tggcaggtaa gttgaatcag ggtaattttt gatgaaattc tgaaatgcca
     1201 ctattgcttc atcctggcgg gatttatcct gcaccagcgc aattgctgca ttgtaatccg
     1261 tgtttgcatc gccgcttttc accggcgcgc cagcattcgc agtaccagca tcagctgtcg
     1321 gcgtcgttga tgccgccgca ccgctttgat caccgctggt tgattgcgcc gctgcaccac
     1381 cgctgctgag gctatcgatc tgcaacagga tctgcttttg ccgctccacg acctgattca
     1441 gttgatactg attttcctga atctgaccgc gcagggaatc aatatcggat tgattatcag
     1501 agagttgttg ctggagttgg gttaaaagct ggctgtgagc atttgaaata cgctcaagtt
     1561 gagtgacgcg gtcttcgacc gagcctgagc cgacactact gattggtgcc tgagcaaaag
     1621 cggcccaggg ggccgctata ccaaccagta acgacagact caatagttga tgtctgaagt
     1681 tactgctcat gcaattctct tagtaaacca gtaccgcacg acggtttttg gagtatgccg
     1741 cttcgtcatg acccagtact gcaggttttt ctttaccgta agaaacgatg gagatctggt
     1801 ctgcagaaac gcctttaccc tgcaggtaca tcttaacggc gttcgcacga cgttcaccca
     1861 gggagatgtt gtattccgga gtaccacgtt cgtccgcgtg accttctacg gtgactttgt
     1921 aagacgggtt gctacgcagg aagtttgcat gtgcatccag catttgagcg aagtcagaac
     1981 ggatatcgta cttgtccaga tcgaagtaaa cgatgttgtt ctgctgcagc tgttgcattt
     2041 gcagacgagc ctgctcttcg gaagacatgt tgccgttgcc gccgttcgca tccataccag
     2101 tgccggcacc cagcatgcct tcgctgccgt cattgctggc gttcttattg gaagaacatg
     2161 ccgcaattgc cataacaggc agagcaatca tcagcccttt cagcactttg ttcagttgca
     2221 tttcaatgat tcctttacta ttcaattaat tattatcaca gatacggcga ccaggcaggg
     2281 aatttgacct gtccatcagt tgccggaaga cgcgctttga aacgcccatc tgtagaaacc
     2341 aaattcagca cggatcccat cccctgagaa gagctgtaga ttaccatagt gccgttaggt
     2401 gccagacttg gcgtttcatc caggaacgtg gacgacagaa cttgtacgcc tcccgttgcc
     2461 agatcttgtt tggcaatgtg ctgctgccca ccattggagc tgaccattac cataaattta
     2521 ccgtcgctgc tgacatccgc atcctggttc tgcgaacctt cccaggtaat acgttgtggc
     2581 gcaccgccgt tgatattcac tttataaacc tgtggacgac cggcctggtc agaagtaaat
     2641 gccaggttct ggctgtccgg gaaccaggtc ggttcggtat tgttactgcg accatccgtc
     2701 acctggcgga tctgaccaga agccaaatcc attacgtaca ggttcagact accggttttc
     2761 gacaaggcga atgccagttt gctgccgtct ggcgagaatg caggtgcacc gttgtgacgc
     2821 gggaatgaag ccacctgacg tacagcgcca tttgccagcg tttggatgac cagcgcggaa
     2881 cgaccgcttt cgaaggtcac ataagccagt ttagaaccgt ctggtgacca cgccggagac
     2941 atcagcggct gcggtgaacg gtgaacgaca aactggttgt aaccgtcata gtcagatacg
     3001 cgcagttcat acgggaactg accgccgttg gtctgaacaa cgtaggcaat acgggtacgg
     3061 aacgcacctt taatgccggt cagtttttca aacacttcat cactggcggt atgaccagca
     3121 taacgcagcc actgcttgtt cactttgtac gagttctgag caagtacagt acccggtgcg
     3181 ccgccagtgt caacaagttg ataagcaaca ttgtaagaac catccggatt cggagtgacc
     3241 tgaccgacaa ctacagcgtc aatacccagc gcggaccatg cagctggttg tacttcctgc
     3301 gcactacccg gctgctgtgg cagacgagcg cgatctaacg gattaaattt accgctgtta
     3361 cgcaagtcgg cagcaacgat gccgccaata tcttcaggtg ccgcaccagg gcccgcccac
     3421 tggaaaggaa caactccaat aggacgaccg gaatctacac cgctgtcgat cacaatgcgg
     3481 acttcagcat gcagaactga tgcccacagt atgagaaaac caaatgctac tcgtaatgcc
     3541 tgcttcatca tatctccctt atctggaccg atggcccacg ataatttagc agaatgttaa
     3601 caaactaaaa tacacaaaac taccagaacc ccgtggcaac cggtgcctga tgttgaccgt
     3661 cggaacagtc aacatcgcga ttacggtttg aagtccaatg gcgcgttttt gaacacttca
     3721 tatactgcct ggcttggtgg tttcgggatc ttcgcaagtt tagctgctgc caacgcagcc
     3781 tgacaaagtg cgggatcgcc accttcaggt ttgatatcca gtaacatacc atcgggagcc
     3841 agttttatgc gcagcgtaca ggttttgcct gcataggacg atgcgtcata gaacttactt
     3901 tcgatagcag atttaatctg cccggcatag ttattgatat cggcccctga tgcgccattg
     3961 tttttagtat taccactccc ggctggcgaa gcattgttcc ctttcgcccc tccccccgtt
     4021 ttcggtgcat tcttaccaga gcttagctca ccgaaaatat catctgcctc tgcggcagct
     4081 tttgctgcag cggctttttc agctgctgct tttgcagctg ccgctttttt gtctgcggcg
     4141 gctttttctg ccgctgcttt cttatcagct gctgcctttt cagcagccgc tttttctgct
     4201 gcttttttgt cggctgcagc tttctctgct gccgctttct tatcagctgc agccttttca
     4261 gcagccgctt tcttctcagc ttctgctttg gctttttcag cagcttcagt tgccgctttc
     4321 tttcttgctt cagctgcagc tgcttctgcc gcttccgctt tcttcttcag tgccgccgcg
     4381 gctgcctcgg cttttttctg cgcttcggct gcggctttgg cggcttctgc ttctgctttt
     4441 ttcttcgcgt ctgcagccgc tttcttcgca gcttcttccg cagctttagc atccgcttcg
     4501 gccttcgctt tagcatctgc cgccgctttc gctgccgctt cttccgcttg cttctgcttt
     4561 aactcggcct gttttgcggc ttcttcagcc tgctttttct gctcctgagc agctaaccgc
     4621 tctttctcaa gttgcttcag gcgttcctgt tcagccgctt gtttctcacg cagctcttcc
     4681 gcagcctgct gttccttcat cttgcgctgc tcatcagaac gcttcgcgct tgattcctgg
     4741 ctttgcatgc gtttgtactg ctcaactacc gcacctgaat caaccatgac agcgtcgatg
     4801 gatgaaccac cgccgcctcc ggctgaagct tctatattct catcgaacga actccagatc
     4861 agcgccgcaa ataagatgac atgcagcact gctgaaataa ttatcgcccg cttgagcttg
     4921 tcgttttgtt cggttgcctt tgacactctc ggtttccaaa aactgttcgc ctgttacccg
     4981 ctctctttca agcaagggaa acgcagatgt ttagataggc tgcgtcatta aaccaaccga
     5041 tttcacaccc gcactatgta acaagttcag tgctttaatt atttcatcgt aaggcacatc
     5101 ttttgcgcca ccgatcagaa agaccgtttt cgggttggcc ttgaaacggc tggacacttc
     5161 cgccaccacc tgctctggtg gtaaacgctc caggcgatct ttctcaacca ccacggtgta
     5221 ctgaccaata ccagacactt caacaatcac tggcggatta tcgttactgc tcaccgcctg
     5281 tgattcagta gcgtctggca gatcgacctc cacgctctgg gtgatgatgg gcgctgtcgc
     5341 cataaagatc agcaacagca ccagcagtac gtccagcaac ggtacaatgt tgatttcgga
     5401 cttgagatcg cgacgacctc gtccacgcgc tctggccatg gcttacccct tgttgctctc
     5461 gctaacggta aacgcctggc ggtgcagaat cgcggtaaac tcttccataa agttgtcgta
     5521 attcagttcc agtttgttta cgcgctggtt gagacggtta taagccatta ccgccggaat
     5581 tgcggcgaac agaccaattg ccgtcgcaat caacgcttct gcgataccgg gcgcaaccat
     5641 ttgcagtgtt gcttgtttta ccgccccgag ggcgataaag gcgtgcatga tcccccagac
     5701 cgtaccaaac agaccaatat acgggctgat ggagccaacc gtaccgagga acggaatgtg
     5761 cgtttccaga ttttcaagtt cacggttcat ggagatacgc atagcacgcg acgccccttc
     5821 cactacggct tccggcgcat ggctattggc acgatgcagg cgcacaaact ctttgaaccc
     5881 gctgtaaaag atttgttccg aaccagtcag attatcccgt ttcccctgac tctcttgata
     5941 gaggcgagag agttcgattc cagaccagaa tttatcttca aacgcttcgg cttcacgcgc
     6001 cgctgcgtta agaatacggg tccgctggat aataatggcc caggatgcga ttgaaaaacc
     6061 aatcaaaatc aacatgataa gtttaaccag aaggctagcc ttcaggaaca aatcaaggat
     6121 attcatgtca gtcactgctt aaactccgcg acaatagact tgggaagcgc acgaggcttc
     6181 attttgagtg ggtcaacgca aacaaccaga acctctgctt cattcagcaa agtattctcg
     6241 gcgttgacaa tacgttgcgt gaaaaccaaa gaggtgccac gcattgatgt tatttcagtc
     6301 tgtatttcga gcatatcgtc gagccgcgca ggtgcgtaat attccaccgt cattttacgt
     6361 accacaaagg caacgcgttc agccatcagc gcctgttgac tgaagtgatg atgacgcagc
     6421 atctctgtgc gtgctctttc ataaaaagcg acgtaactgg cgtggtacac cacaccaccg
     6481 gcatcggtat cttcatagta gacgcgaacc ggccatcgaa acagcgttgt attcacttta
     6541 catcccggta atgcaacaaa agttagagct tttaaacgct gctactatac gcgcgcgatt
     6601 gaggtttggg aatgagggca agttaaggga gcgtaaaaat ttatgggcct ggagcggccc
     6661 ataatttttt aaagagaatt ctgatttaaa agaagaagaa aatcagcccg acaatgagaa
     6721 caatatcggc aagcaatggg caaaaaatcc cttgccaaag aaccttctgc ggacgaaagc
     6781 ccacgccgtg aatcacacca gcacagacgg cccacatcag caataaacca tgccagattt
     6841 ccagttcact ggtcttcgcg gcgaaacgtg atgggtccca aaacatacat cctgctaaca
     6901 gaagcgccat cacgaaggaa agcgcccgca gggggcgctt gtccattacc gcatacaagg
     6961 ttgcgataat cttactcatc agatgtcttc ttgaccggct ttgcctgatt cgacgtgctc
     7021 aagcgccagc gcggtgatta ccccaaacga acaggcaaga agcgttccca gaatccatgc
     7081 gaaataccac atttttagct ccttacttag tacagagagt gggtgttacg ttcaatatct
     7141 tctttggtga tacgaccgaa cattttccag taacaccagg cggtgtagag cagaatgatc
     7201 ggtaccagaa ccaccgcaac ccaggtcatg acgttaagcg tcagctggct ggaagttgca
     7261 tcccacattg tcagacttgc gttcatcatg gtgctggacg gcatcacaaa cgggaacatt
     7321 gcgataccgg ctgtcaggat gatgcaggcc agcgtcaggg aggagaacac aaacgcccac
     7381 gccgctttat ccatacgtgc agtcaggatg gtcagcagcg gcagaaccac acccagtgcc
     7441 ggaatagccc acagaattgg cgtgttgttg aagttaacca gccatgcgcc agcttcacga
     7501 accacttctt tattcagtgg gttagaggct gcgtaatggt ccattgtcga tttcacgaca
     7561 taaccatcga taccgtacat cacccatacg ccagccagtg cgaaacagac cagtgtcacc
     7621 agtgccgcca cctgagccgt tgcacgggta cgcaggtgca gttcgcccac ggtacgcatt
     7681 tgcagatagg ttgcgccctg agtaatgatc atccctacgc tcaccacgcc tgccagcagg
     7741 ccgaacgggt taagcaggtg gaagaagtta ccggtgtagt acagacgcag atattcatca
     7801 acgttgaacg gtacgccctg caacaggtta ccgaacgcta caccaatcac caacggcgga
     7861 acgaagctac caatgaagat gccccagtcc cacatgttac gccagcgggt ttcttcaatc
     7921 ttggagcggt agtcaaaacc gaccggacgg aagaacaaag acgccagcac gaggatcatc
     7981 gccacataga agccggagaa cgcagcggca tagaccatcg gccaggcagc aaagagtgcg
     8041 ccgcccgcgg tgataagcca tacctggtta ccgtcccagt gtggtgcaat ggagttaatc
     8101 ataattcgac gctcggtgtc gttacgaccg aggaaacgag tgagcatgcc cacccccatg
     8161 tcgaaaccgt cagtgactgc aaaaccaatc agcagaacgc caaccagcag ccaccagata
     8221 aaacgcaata cttcataatc gatcatttga cgactcctgt cttagcgtgc cggctgagta
     8281 gtcgtggaag actgctcaaa gtgatagcga ccggttttca ggctgcttgg gccgaggcgt
     8341 gcaaatttga acattaagaa caattctgcc accaggaaca gggtatacag gccgcaaatc
     8401 agcaccattg agaagatgag atcgcctgcg gtcagtgacg agttcgccac agctgtcggc
     8461 agcacttcac cgatagccca cggttggcga ccatattcag ccacgaacca gcccgcttct
     8521 acagcaatcc acggcagcgg aataccgtac agcgcggcgc gcagcagcca ttttttctcg
     8581 ccaatgcggt tgcggatgac actccagaaa gagagcgcga tgattgccag cagcaggaag
     8641 ccacacgcca ccatgatacg gaacgcgaag tacagcggcg ctacacgcgg aatggagtct
     8701 ttggttgcct gttgaatctg cgcttcagtc gcatcagcca cgtttggcgt atagcgtttc
     8761 agcagcagac cgtaaccgag gtctttcttc atgctattga actggtcacg aaccgcctgg
     8821 tcggtagaac cagaacgcag ttgctcgagc agagagtacg ccttcatccc gttacgaatg
     8881 cgttcttcat gctgcaccat cagttctttc aggccgataa ctggagtatc cacggaacgc
     8941 gttgcaatga tgcccagcgc gtaagggatc tggatcgcaa atttgttcgt ctcctcttcc
     9001 tgatcaggaa tgccgaacag agtaaaggca gcaggcgcag gttgcgtttc ccactcggct
     9061 tcaatagcag ccagtttggt tttctgcacg tcgcccattt cgtaaccaga ttcatcaccc
     9121 agaacaataa cagacagaac ggcagccata ccgaagctgg cagcgatagc aaaagagcgt
     9181 ttagcgaagg cgaagtcacg acctttcagc atataccatg cgctgatacc gaggatgaac
     9241 atcgcgccag tcacataacc agacgctaca gtgtgaacga atttcacctg agcaaccggg
     9301 ttaagcacca gctcggagaa gctcaccatc tccatacgca tagtttcaaa gttgaaatcg
     9361 gacgcgattg ggttttgcat ccagccgttc gcaaccagaa tccacagtgc ggacaggttt
     9421 gaaccgagcg ccaccagcca ggtgacacac atatgctgaa ctttacccag acgatcccaa
     9481 ccgaagaaga acagacctac aaaggtggat tcgaggaaga aggccatcag accttcgatt
     9541 gccagcggcg caccgaagat atcccctaca tagtgggaat agtaagacca gttagtcccg
     9601 aactggaact ccatggtcag accggtagcc acacccagag cgaagttgat accaaacaac
     9661 ttgccccaga acttggtcat atctttataa atctgtttgc cggaaaggac gtagaccgtt
     9721 tccataatgg ccagcaggaa cgccataccg agcgtcagtg gcacaaaaag gaagtggtac
     9781 atcgcggtca aggcaaactg taagcgcgac agttcgacta tatctaacat catgactcct
     9841 tgctcatcgc atgaagactc cgagagtgaa ccccgttaga aagggtcaca cgcatgcccc
     9901 aatacaaaat tatttgctcg cctctatcgt tgccatttca tcaaaagagg aaaagacaat
     9961 gataaaaaag ttacaaatgt gttaatgaat aaacc
//
LOCUS       DAZUCC010000185         9939 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZUCC010000185 DAZUCC010000000
VERSION     DAZUCC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.186"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..591)
                     /gene="yeeO"
                     /locus_tag="ACHY09_003973"
     CDS             complement(<1..591)
                     /gene="yeeO"
                     /locus_tag="ACHY09_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFS3886874.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVVMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLIN"
     gene            752..827
                     /locus_tag="ACHY09_003974"
     tRNA            752..827
                     /locus_tag="ACHY09_003974"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:785..787,aa:Asn,seq:gtt)
     gene            complement(865..1815)
                     /gene="cbl"
                     /locus_tag="ACHY09_003975"
     CDS             complement(865..1815)
                     /gene="cbl"
                     /locus_tag="ACHY09_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFS3886875.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1917..2834)
                     /gene="nac"
                     /locus_tag="ACHY09_003976"
     CDS             complement(1917..2834)
                     /gene="nac"
                     /locus_tag="ACHY09_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFS3886876.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3161..3236
                     /locus_tag="ACHY09_003977"
     tRNA            3161..3236
                     /locus_tag="ACHY09_003977"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3194..3196,aa:Asn,seq:gtt)
     gene            complement(3291..4226)
                     /gene="ldtA"
                     /locus_tag="ACHY09_003978"
     CDS             complement(3291..4226)
                     /gene="ldtA"
                     /locus_tag="ACHY09_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS3886877.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(4288..5367)
                     /gene="cobT"
                     /locus_tag="ACHY09_003979"
     CDS             complement(4288..5367)
                     /gene="cobT"
                     /locus_tag="ACHY09_003979"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFS3886878.1"
                     /translation="MQILADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAILVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPISGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPRDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5379..6122)
                     /gene="cobS"
                     /locus_tag="ACHY09_003980"
     CDS             complement(5379..6122)
                     /gene="cobS"
                     /locus_tag="ACHY09_003980"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFS3886879.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLVKILVLSELTLRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVVIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6119..6664)
                     /gene="cobU"
                     /locus_tag="ACHY09_003981"
     CDS             complement(6119..6664)
                     /gene="cobU"
                     /locus_tag="ACHY09_003981"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFS3886880.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WNYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6798..7235)
                     /locus_tag="ACHY09_003982"
     CDS             complement(6798..7235)
                     /locus_tag="ACHY09_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFS3886881.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLNGGSVQSLSDAYSDYSVLLLIAAESEISVPEWLPHSEGTTVIVIAEHNARLLTGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(7453..7628)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(7717..8631)
                     /gene="pocR"
                     /locus_tag="ACHY09_003983"
     CDS             complement(7717..8631)
                     /gene="pocR"
                     /locus_tag="ACHY09_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFS3886882.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSVRQTRAPNPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSENAAMETV"
     gene            complement(8834..9643)
                     /gene="pduF"
                     /locus_tag="ACHY09_003984"
     CDS             complement(8834..9643)
                     /gene="pduF"
                     /locus_tag="ACHY09_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFS3886883.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVTGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
BASE COUNT         2775 a         2616 c         2120 g         2428 t
ORIGIN      
        1 gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc taccaataag
       61 agtgatggca gctgctggat aactgagtac ggtcagctcc agataagtca acgccagtgc
      121 tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc caaaatgatg
      181 aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact gccgcgtcgc
      241 caccctcgct cgtcgtcgat ctcgcttacc gagactaaat gctacaacga cagtagtacc
      301 aagatcgata gctgcaaaaa aagccataac gaccatattg aagctgtccg ccaaccccac
      361 gccggccatc gcatcttttc ccagccagct gaccagaaaa gtgctcagga cccccatcaa
      421 caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct cgcgccagaa
      481 caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa cctggcgtaa
      541 agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca tttcaaatga
      601 tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc aaatgcaaca
      661 aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg acaaaatatt
      721 tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg tagaacggcg
      781 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat ttctgaaaag
      841 cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt cactgttttc
      901 catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaagtt ccagaaagcg
      961 ccagacatag ttacgctgaa gttgccctcg cttcagtccc aaccagacag tattagcatc
     1021 gaaaagatgc cgcgtatcca ggcggattaa attcttttcc tcttgttcgc cgctggattg
     1081 ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga catcagaatc
     1141 ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gcaaatgcgt catcaatgcg
     1201 tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta ttgattccag
     1261 cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac tatggtgcca
     1321 gcgaaaccac gggaaggcga cgagctgcgg gtcattactc aaacgctcgc tggcgatacc
     1381 aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgtac cctggattag
     1441 ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg gcaagctata
     1501 acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt tggtaaacag
     1561 gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa tgaccagtaa
     1621 tgctttgccc ggttcagtca tgcccagcag tcgcttacct cgtcgaacaa atatttcgat
     1681 gccaagttca tcctccagtt cccgaatatg acggctaacg cctgactgtg aggtaaaaag
     1741 catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga ttatctttag
     1801 ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg ttaaagtcta
     1861 atgcccggca taacaaataa taaaaacccg catcttattc catcccgata taacgcttag
     1921 ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga cattaacaac
     1981 tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt taaggacaaa
     2041 gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc gccacataac
     2101 gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat cgctgcggta
     2161 agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg tagggaaaaa
     2221 gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag gttcatttgc
     2281 gcaatagcat taacatcaac gctttgcccc gggcaatctt gagttcctac caggaaaaga
     2341 tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc ataaatcacc
     2401 gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact attttcatga
     2461 agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg catggtgatg
     2521 gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa tgactgacca
     2581 acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg tgcatgggta
     2641 tagagaattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat caaaagctgt
     2701 tgatttaact caccttccag tgtggcaacc tgctggctga gcgctggttg tgcgatatgc
     2761 aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa gtatttcagg
     2821 cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg ccattactta
     2881 caaccagaat gcaagatgct tgccagttct attttagcgt tgatgcgcaa gctaaccaac
     2941 tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca gtcgcattca
     3001 cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa gattgaaagc
     3061 tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa tgcatttcac cctttgacat
     3121 caccatgcac tgccattaat atgcgccccg ttcacacgat tcctctgtag ttcagtcggt
     3181 agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt
     3241 caaaaaagcc tgcttcgagc aggctttttg ctttctaatt accaacgctc ttaaaacatc
     3301 tgtcttgaac cagaactaat ttgcacaggc attcccgatc gatgttgcaa cgcagcattt
     3361 gcgcgattta catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc
     3421 aatggcactt ttcggtcaga ctcatattct gcacgattgc gcgacagcgg ctcatgaact
     3481 tccagccact ttgaaccatc cggttccgtt gtgtatttca ctggctgatc aataatttgt
     3541 acacgagtgc ctacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga
     3601 atacagccct gacttacccg gagcccaata ccaaaattgg cattggtacc gtggatggca
     3661 tacaacctgc caatataaat cgcgtacagc cccatggggt tatcgggccc cgcaggaaca
     3721 aatgcgggca aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc
     3781 caggttggtg cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc
     3841 ccagcctggc cgataccaat aggaaagact tccactgtat tactgtctgg cggatagtaa
     3901 taaagacgca tttcagcgac gttaaccaca atccctttac ggacagtggc gggcaaaatc
     3961 agttgctgcg gaatggtgag ttgtgagcca gacttcggca aaaaaacatc agcgcccgga
     4021 ttcgcttcca gcatattact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc
     4081 tgagtattgt gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct
     4141 ggaggtaatg gataagttac cgccaggcta ttttggctgg caaaaagcag agcaaatgag
     4201 caaagaatat ttacacgacg catcatgtcc ctttcctaag tcgcgaatac tatccgataa
     4261 gtatagcttt tatcagactt ttcgttttta gctgttcaaa tcagaagtcg tattccccgg
     4321 tagaacaata ttactggcag caagttcgcc catattgttg tatatcgcac aagcagcttc
     4381 gatgatgggc atcgccagag ctgcaccact cccctcacct aaacgcatat ccatattgag
     4441 ataaggctcc agccccaaat gcgacagcgc tatacgcgcg cctttttctg ccgacaagtg
     4501 agaaggaatg agatacggtt tgattgcagg agacatctgg caggctgcga gcgcagcagc
     4561 ataagaaaga aatccatcca gcaacacggg taaaccgcag gaagcagcac ctaacatcac
     4621 tccagctatt ccgaccaaat caaatccacc cacttttgcc aggacatcaa caccatcccg
     4681 aggatttggt tgattcaacg taatcgcccg acgcacaaca tcaattttat tagccagttt
     4741 atctgtcggc aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt
     4801 gctgactatt gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag
     4861 cgtgacaccg ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc
     4921 agcctgacgg cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg
     4981 catgttgata agcccggaga taggctcagc agtatcaata ccaacatcaa ttacgtggac
     5041 gttagcgccc gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc
     5101 agcctgtatg gctgtcactt cttttggaga aatagcgacc ccttcctccc agacgccgtg
     5161 atcggcacac ataaccagta tcgctttttt gcccacatgc ggtatgccat tcaaccccgg
     5221 cattcctgcc agttgtatgg caagcgcctc cagctttccc aggctaccaa caggtttgag
     5281 taacccgtca atatgccgtt gtgcacgcga catagcggca ggatcgatgg ctgggatcgt
     5341 attcagtaaa tcggcaagta tttgcatgtc acgtctcttt atagcagtgc cagcaggaaa
     5401 accagttcac caagctctat ggctgctccc agcgtatcgc ccgtttgccc gcccagcgta
     5461 cgtttgagaa gctgaccgag gatgaaaatc accaccatcg tcaccaccat agcagccaca
     5521 ccatgcatac ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag
     5581 gtttgtcgcc catcaatttt gccaatgaac acattgccaa gcccttcttc ccgcgcataa
     5641 cgatgacgat acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc
     5701 gatgcaagga tcggttcgcc acgcagagtc aactcgctta acaccagaat ctttaccagt
     5761 accacaaaaa tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc
     5821 aacatgcgat cgcggctacg tgcagaaaat acgccatcac aggtatcagc gaggccgtcc
     5881 agatggaatc ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt
     5941 acgccacacc atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat
     6001 ccaatcaaag gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac
     6061 cagcgacgcg gtacaggcag acgcgtaata aatgagagca tcgcccaaaa taatttactc
     6121 atttgatttt tactccaata cccgaaacca ccagccatac ttcatttgcc gcagcggcca
     6181 actgctgatt tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca
     6241 caatccccat tcccacttcg ttagtcacta atacaacctt tgcggggcaa cgttggcagg
     6301 cagcaatcaa cgactgaatc tcagcattaa tcgcctgttc catcgcctga taattccatt
     6361 catcagggtc tttatcgccg ccataatcaa acaacagatt agtcaccatt gtggtaacgc
     6421 attccagcaa cacagcctca ttcgggttaa tgtctgcatg aattaattca tcaagatgtt
     6481 gccaacgctc cactgtgcgc cagtgctccg ggcggccttg ccgatgatgt tctatccgtg
     6541 cagccatctc atcatcaagg atttgcgagg tggcgatata cagaacctgt gaagagtccc
     6601 caataagcgc ctctgcgtgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca
     6661 tcataccgac tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc
     6721 caccgttgct gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat
     6781 ccagcattct caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt
     6841 gttaacgtcc gacaaaatgc gtcattgaca gcggtttctc tggcatgaca gatttcattc
     6901 agataatgga tcaatgccac agccccacca gccggaccag tcagcaaacg ggcattgtgt
     6961 tctgcaatca caattaccgt cgttccctca gaatgaggga gccattctgg tacggatatc
     7021 tcgctttcag ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataatgat
     7081 tgcacagacc caccgttaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag
     7141 gttattggca atatttcagc cagatgtttc gccaggtcca catctccaga taagaggcaa
     7201 aaaagagcta tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttagtttcc
     7261 tgtaatgtcg gtctggttga tgagccatga aacaagattg ccacacctta agtgcagccc
     7321 tggtgcctgt cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc
     7381 agggtggtag gggtataaag acgcgccggt actctaccgg acgcttccgg gcaacccact
     7441 tcccaccgaa gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctcc
     7501 cccgaccttc ccaatctcgc gatcagtggt attttcgggt ttgtcagcat tacagcagcg
     7561 ggggctgcga gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta
     7621 tgttatgagc tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag
     7681 cgtttattta aaccttccct ttttttagca tttactctat accgtttcca tcgccgcatt
     7741 ttcactcact tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt
     7801 gcaaaaataa ctggtttgcg aaaaacctaa attacgcgca atgctggcga tgctccagtc
     7861 actatggcac agtaactcac gagcgctggc catccgttgt tggtttaccc acgcattaaa
     7921 accgattcca tggtatttct tgaataactt actgaaataa taaggactta gataaacgtg
     7981 cgaggcgaca tcttccagac gcaaatcgtc tgataaatgc gcatcaatat agcgtaaagc
     8041 ttttttcatt ttattgtcgt ggggattagg cgcacgagtc tggcgcaccg aatctgactg
     8101 agatttgttc tctttaataa ccacaaaatt gagttgtttt ttgaggcaat tttccacaat
     8161 caacttcaac aaatcagcag aggccataac tcttgagtaa tccatctcag gaactttgcg
     8221 gaattcatta agtaattcgg gatctgcctg ccagcaatca tcgacattca gaatatcgac
     8281 caaatcgaca tcatcattac gcagacgaac ttgcccgcac aatacaaaac caactaaatg
     8341 tccggcgatt accagtggaa tagagaaatc ggttaatccg gcatgacagc gataaataca
     8401 aggctgatcg gatttagagg cttccagacc cccacaacga tcgctcatgc gacatcgagt
     8461 gctcagctga gggtgttgcc gcatcatctg acaaaatgga gtgaagttaa acagctcgga
     8521 aatttcatcg ccgtgaatat tgaccacgac aaccgcaagg ctggtcgcct gcgcaaaatc
     8581 ctgcgcgatt ttattaatga gttctgaatt aagtgtcgcc gcagaaatca tgataaaacc
     8641 tcccagttaa tttattgtta tagcaaaaca attgttttac atttaatctt cacatcatcc
     8701 ttgatgattt actgacgaga agcacaaagg acaataacaa aagcctccgt gaagcgggag
     8761 actttttaat aagttcggaa agcagatcac aaacagtaaa aaaataaacg tttataactg
     8821 ccagattatt tcgttattcg ctgatttctt taggcgttac actattgaca ggtaaattct
     8881 ttgcaatgag atagcgataa atcgccgcac caacacatgc cccaatgatc ggtgcaataa
     8941 tgggtacaat gaaataagga atattgcgtc caccagtcat cgcaatttcc ccccaacccg
     9001 caaaccaggt gaaaatttta ggtccaaaat cacgcgctgg attcatggca aaaccggtta
     9061 atggtccggt agaagcacca ataacagcaa ccagaatacc aatgagtaaa ggcgcaagcg
     9121 gtcctttggg tacgccatta ccgtcatcgg tcaacgccat aatcaacccc attaacatgg
     9181 acgttatgac cacttcgacc aacgctgctt gccatacatt aatcgccgct gctggataag
     9241 tactgaagat actggcgagt tgtaaacttt ctgcgctacc acgcactata ttgtgagcag
     9301 attcaaaatc ggtaaacaaa ctgctgtaga gagaatatgc cagaaccgca ccaccaaagg
     9361 cacccgctaa ttgcgccaca caaaatggca gcactttacg ggctgggaaa gaggcaaaca
     9421 gccacagagc aatggtaact gccgggttga gatgagcgcc ggaaatacct gccgtaaggt
     9481 aaacagcaag cgaaatccct aatccccaaa tgatacaaat ttcccacagt cccagacttg
     9541 ccccggtaac cttcagggcg cacaaacaac caatgccaaa aaagaggaac agtccggtgc
     9601 ccaaaaactc ggcaacgcat tgcgccttga gtgaatcatt catagtgaca ccttctttac
     9661 ggagtttttc tttatcagcc aactatgcgc tgattgttgt gtataccgag cgcaactata
     9721 aaaaattgct ctgcagaaaa tattgccaac atgcatgaca gactcgtgta aaaatcgtgc
     9781 taacataaca aattattgtc attgccgaat catgcaacac agcgttaatc agtgttgaaa
     9841 gtaataattt ccaccaaact tttacaataa aaaattgcca tcatgacata ataaaattga
     9901 aacaaatatc acagttcctt ccttcgaacc aaactcaac
//
LOCUS       DAZUCC010000186         9829 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZUCC010000186 DAZUCC010000000
VERSION     DAZUCC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.187"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(30..1190)
                     /locus_tag="ACHY09_003985"
     CDS             complement(30..1190)
                     /locus_tag="ACHY09_003985"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFS3886884.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(1196..1867)
                     /locus_tag="ACHY09_003986"
     CDS             complement(1196..1867)
                     /locus_tag="ACHY09_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFS3886885.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCIAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(2015..3496)
                     /gene="tolC"
                     /locus_tag="ACHY09_003987"
     CDS             complement(2015..3496)
                     /gene="tolC"
                     /locus_tag="ACHY09_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFS3886886.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            3701..4330
                     /gene="nudF"
                     /locus_tag="ACHY09_003988"
     CDS             3701..4330
                     /gene="nudF"
                     /locus_tag="ACHY09_003988"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFS3886887.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            4331..4753
                     /locus_tag="ACHY09_003989"
     CDS             4331..4753
                     /locus_tag="ACHY09_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFS3886888.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            4778..5605
                     /gene="cpdA"
                     /locus_tag="ACHY09_003990"
     CDS             4778..5605
                     /gene="cpdA"
                     /locus_tag="ACHY09_003990"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFS3886889.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            5605..6186
                     /gene="yqiA"
                     /locus_tag="ACHY09_003991"
     CDS             5605..6186
                     /gene="yqiA"
                     /locus_tag="ACHY09_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFS3886890.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            6215..8107
                     /gene="parE"
                     /locus_tag="ACHY09_003992"
     CDS             6215..8107
                     /gene="parE"
                     /locus_tag="ACHY09_003992"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS3886891.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFTLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(8152..9021)
                     /locus_tag="ACHY09_003993"
     CDS             complement(8152..9021)
                     /locus_tag="ACHY09_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HFS3886892.1"
                     /translation="MNNKFKIILWMMLSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWSSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
     gene            complement(9035..>9829)
                     /locus_tag="ACHY09_003994"
     CDS             complement(9035..>9829)
                     /locus_tag="ACHY09_003994"
                     /inference="COORDINATES: protein motif:HMM:NF017942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFS3886893.1"
                     /translation="GIIRWCIRSPKIYFILKGNIMNSYLKISLAGITMLSCLSPTLVC
                     AEQPHGFIDYRHEYLDDTRTHADRVEFGTFFSNGIGLMGELRYNTDEGDKDKWDPSQF
                     GNNGTGLSVVYRFKPLDDKKFWLEPMFWLDTTQYWSTYEYGLSAGYDFSKEWKVSGRF
                     RYDMDKATDKSKGYGNDDRNNRRYDVWIDYRPQKTNFQYQLNLVYYNNGYLTWNDGHK
                     NYTASFKVGYKMGSWIPYMSIADYKGVDKTSSNRQIRWRWGLTYTF"
BASE COUNT         2349 a         2390 c         2554 g         2536 t
ORIGIN      
        1 cccgcttatc ggggcaatat taagctgtat tactcaacaa aaatatgcgg ataaaaccga
       61 gacatatcct gggtgatcaa tgcacggtct tcacgaatgc caattccggc aggttgatcg
      121 ttcaccagcc agctaccaat cagcatatag ctgtcgccga atttcggtaa cgggtggaat
      181 tgctgaacaa tcatcccttc ttcgccatac ggaccttccg ctgcttcaat ggttttgccg
      241 ttctcaatga tcgacacgtt tgcgccttca cgggagaaga tcggtttaac cacatatttt
      301 tccatttgcg gatgatcatc ttccgcaaaa taagcaggca gcaggttcgg gtgattcggg
      361 aacatctccc acagtagcgg cagaagcgct ttgttggaga taatgctctt ccacgccggt
      421 tccagccagc gtacgcctgc atcctccagc ttggtggaga acatctcgcg cagcataaat
      481 tcccacggat acagcttgaa caggttggaa attacctgat cctgtaaatc cgtgaactga
      541 cctttttcgc ctaaaccgat atcgtcgatg tagaggaatt cggtagcgat ttcagcttcc
      601 gtcgcgcagt cctgcaaata ctgaatggtt ccgcgatctt ccaccgtgtc gcgacagcag
      661 gtgagatgca gcaactggaa gccatactgt tcacgcaact cgacaaagcg atcgattagc
      721 ttttcttgca gactgttaaa ctggtcgctg ccctccggca agttaccggc gttaagctga
      781 tcttccagcc agatccactg aaagaacgcc gcctcgtata gtgacgttgg tgtatcggcg
      841 ttattttcca gcagtttagg ttcgccagtg ccatcccacg ccagatcaag acgcgaataa
      901 agcgatggct ggtgagtcag ccatgactga cgcacaaaac tccaggtgtg ttttggaatg
      961 cggaatttgg tcatcagctc atcgctggcg atcacttttt ccaccacttt caggcacatc
     1021 tggtgcagtt cggcggtgac ttcttccagc ttttcaacct gggcgagggt caacttgtag
     1081 taagcatctt cacaccagta cggctcgccg tacatggtgt gaaaattgaa accgtattcg
     1141 tgggctttct cacgccagtc cgggcgctcg gtaatactga ctctttccat cggtatcagc
     1201 cacccattga acgagaagag gtgccggttg cgctacgctg catagtgctt tgtttggcaa
     1261 cagattcacc aaaaccgcca cgggtaacgg tagtggtggt cgccggtttt ggtgccatgg
     1321 ctgtcttcgg tacggtcatg gtgcggcctg gctgggctgc gccatagttt ttacccgtcg
     1381 cgtcggtata tttaccgtaa gccgggctgg ctgggttttt cgaggagaac agcggctgct
     1441 gtgcaaatcc cgcaccgccg cccatcagac gacccatcat gtaaccggcc atcagcggca
     1501 tccagaaact cccgctggat tgctgggcct gcgcctggtt ttccggtgcc atgccagcct
     1561 gggctggtgc ctgctggcac tgaccttcgc caaattcagc aatacagtct tcacgagtgg
     1621 cgtatttcgg cgcagtacgt tcggcttctt tcagcgcatt gttgtacgcg gtggtacatt
     1681 cggcgctttt gcctgggttt gcagctgaac agtcgtcagc attttgatag agagacactg
     1741 tttcatcact cttttcacag ccagccagca taaaaacagt ggcaaccgcg agagcgactg
     1801 gtgtcagatg gcgtgcgctc cagtttttgc ggaacgatgc gtggcgtatg gattttgtcc
     1861 gtttcatttt tgtcttccgg gaccagtggt aaatacccat cagaatagag gatggctggt
     1921 cgaaattgaa gcgagaaggg ggaagaatgc ggcagataac ccgtatcttt acgttgcctt
     1981 acgttcagac ggggccgaag ccccgtcgtc gtcatcagtt acggaaaggg ttatgaccgt
     2041 tactggtggt agtgcgtgcg gatgtttgct gaacgacggg tgccgggcta tcaggcgcat
     2101 aaccatcagc aatagcattc tgttccggcg tttgcggggc aacgttttcc ggattagtgg
     2161 aaaccggttt gctcagcgca ttgttcagtg ccagaagatc ctgctcgttc aacgtaccca
     2221 gagctgattt aatattcaac tggttaatca ggtagttata acgcgcattc gccagctctt
     2281 gcttggcgtt gtacagcgtg gtggtcgcat ccaacacatc aacaatggta cgcgtaccga
     2341 ccgagtaacc cgcttccatc gcgtctaatg agctttgagc ggaaactacg gcttgtttgt
     2401 aggcgttaat gctactgata gaagcattaa tgttgttgaa ggaggaacgt acggtttgca
     2461 cgacgctacg atgcgcgctt tccagttgct cgctggcacc aacaaagttg tactgggcct
     2521 gtttcacctg cgagttaacc attccgccct gataaatcgg cagcgagaag ctcaggccaa
     2581 ctttgttctg gcccatattg ctgtcgtcat actgggtacc agcggcacca cgggttttcg
     2641 aaccgctata agaggtgtca gaaatcccgg tagaagccgt taaatccaga gtcggtaagt
     2701 gaccatcctg cgcctggcga atttgctcgc gcgccaggtc ctggctcaag cgcgcctgta
     2761 acagcgacag gttgcgtttt tcggcttctt tcagcagcgt gttaaccggc tgtggtttgt
     2821 cggttttaaa gttttcgaca ttcagcgccg ccagttctgg atagtagtta ccggtgatct
     2881 ggcgcagctg ctctaccgcg ttatcaaggt tattacgtgc ggtcacttcg ttcgccagca
     2941 cggtatcgta ctgtgcgcgg gcgttctgca cgtcagtgat cgctaccagg cccacgttaa
     3001 aacgttgggt ggtttgatct aattgacggt agatcgcttc tttttgcgcc tgtgtatagg
     3061 aaagaacgtc aatagcattc aacacgttga aataagcggt cgcggtgttg aggatcaagg
     3121 tttgttgatc ggtctgatac gtgacgtcct gaatcccagc tgctttttcc tgcagcgtta
     3181 acgcacgcca tttcgacata tcaaaaatgg attgagttaa ctgcagggac gcactggtcg
     3241 cgttagagtt gatgccgttc gcgtcgcggt agccgttgct ataggtgtaa tctgcaccta
     3301 aacctagctg tggcagtaat ggactgcgcg cttcattaat tttttcaaag gcagcatcac
     3361 gatcggcggc agacttacgc aattccgggt tactaaggcg tgcttgctga taaacttgca
     3421 tcaggttctc ggcctggctc aacgaactga aaccagaaag gctcaggccg ataagaatgg
     3481 ggagcaattt cttcatttgc attccttgtg gtgaagcagt atttagcgcg atcaaactgt
     3541 aaaattattg cggattctag cagaagccgc taccgcaaaa ggttggcgtt acgtgccata
     3601 gggcgttaat gtgcctcaat ttaacacatg actgctcaaa cggcagtcaa acgtcgctga
     3661 aattcacatt taattcacta ttagtgccag gacattaaca atgcttaagc cagacaacct
     3721 gcccgttaca tttggcaaaa acgatgtaga aattattgca cgagaaacac tttatcgcgg
     3781 ctttttttca ttagatcttt atagatttcg tcatcgtcta ttcaacgggc aaatgagtca
     3841 tgaggtacgg cgggaaattt ttgagcgcgg tcacgccgca gtcttgctac cctttgaccc
     3901 agtgcgtgat gaagttgtgc tgattgagca gattcgaatt gccgcgtacg acactagcga
     3961 aaccccctgg ctactggaga tggttgccgg gatgattgaa gagggtgaaa gtgtggaaga
     4021 tgttgcccgt cgcgaagcga ttgaagaggc gggactgata gtcaaacgga ccaaaccggt
     4081 gttaagtttc ctggcaagcc cggggggcac cagtgagcgt tcgtcaatta tggtgggcga
     4141 agtggacgcc acgaccgcaa gcggtattca cggtctggct gatgaaaacg aagatattcg
     4201 cgttcatgtg gtaagccggg aacaggcata ccagtgggta gaagagggga aaatcgacaa
     4261 cgcagcgtcg gtcatcgctt tgcaatggct gcagctgcat catcaagcgt taaaaaatga
     4321 gtgggcataa atgaagcgtt acacacctga ctttcctgaa atgatgcgcc tgtgcgagat
     4381 gaacttttca caattgcgcc gtttgttacc gcgcaatgac gcacccggcg aaactgtaag
     4441 ctatcaggtg gcaaacgcac aatatcggct gacgattgtg gaatcgaccc gatacactac
     4501 cctggtgaca atagaacaga ctgcgcccgc gatcagttac tggagccttc cgtcaatgac
     4561 ggtgcgtctg tatcatgacg cgatggtggc tgaagtgtgt tcaagccagc agatttttcg
     4621 cttcaaagcg cggtatgatt atcctaataa aaagttgcat caacgcgacg aaaagcatca
     4681 aattaaccag tttttagcgg actggttgcg atactgttta gcacatggag cgatggcgat
     4741 tccggtttac tagcgtcgtg aaacctaagg acaccatttg gaaagcctgt taacccttcc
     4801 tctggctggt gaggccagag tcaggatttt acaaattacc gacactcacc tgtttgcaca
     4861 aaagcacgaa gccctgttag gggtaaacac ctgggaaagc taccaggcgg tgctggaggc
     4921 gattcgtcca caccagcacg aattcgacct gattgtcgcg acaggtgatt tagcgcagga
     4981 tcaatcctct gcggcctatc agcatttcgc tgaaggcatc gcaagttttc gtgcgccctg
     5041 cgtctggctg ccgggcaacc acgatttcca gcccgcgatg tacagcgcgt tacaggatgc
     5101 gggtatctcc ccggcgaagc gggtgtttat tggtgagcaa tggcaaatcc tgttgctgga
     5161 tagccaggtg tttggcgtgc cgcacggtga gctgagcgag tttcagcttg agtggctgga
     5221 acgtaaactg gccgatgcgc cagaacgcca tacgttgctg ctgctgcatc atcatccgct
     5281 acctgcgggt tgtagttggc ttgatcaaca cagcctgcgt aacgcgggcg aactggatac
     5341 cgtgctggcg aagtttccgc acgtcaaata cttgctgtgc ggtcatattc atcaggagct
     5401 ggatctcgac tggaacggtc gccgcctgct ggcaacgccg tcgacctgtg tgcagtttaa
     5461 gccgcactgt tccaacttta cgctggacac catcgcgccc ggctggcgta ctctcgagtt
     5521 acatgctgat ggcacgctga ccaccgaggt gcatcgcctg gcggacacac gtttccaacc
     5581 tgataccgct tcagaaggct actgatgtct acgcttcttt atttacacgg tttcaacagc
     5641 tcgccgcgct ctgcaaaagc gagcttgtta aaaaactggc ttgcggaaca tcaccctgac
     5701 gttgagatga tcatcccgca gttgccgccg tatccttccg atgcggcaga actgctggaa
     5761 tccattgtcc tggaacatgg cggcgattcg ctgggtattg tcggttcgtc actgggggga
     5821 tattacgcca cctggttgtc gcaatgtttt atgctgcccg cagtggtggt aaacccggcg
     5881 gtacgcccgt ttgaactgct gacggactat ctcggtcaga acgagaaccc ctacaccggg
     5941 cagcaatatg tgctagagtc acgccatatt tacgatctca aagtcatgca gattgacccg
     6001 ctggaagcgc cggatttgat ctggctgctg caacagacgg gagatgaagt gctggattac
     6061 cgccaggcgg tggcgtacta cgcttcctgc cgccagactg tcatagaagg cggcaatcac
     6121 gcattcacgg gcttcgaaga ttatttcaac ccgatcgtcg attttcttgg tctgcaccat
     6181 ctctgacgat caactcgaat tgctaactta aaccatgacg caaacttata acgctgatgc
     6241 cattgaggta ctcaccgggc ttgagccagt tcgccgccgt ccggggatgt ataccgatac
     6301 cactcgccct aaccatttgg ggcaagaagt tattgataac agtgtcgatg aagcactggc
     6361 gggtcacgca aaacgcgtgg acgtaatctt acatgctgac cagtcgttag aagtgattga
     6421 cgatgggcgc gggatgccgg tggatattca cccggaagag ggtgtaccgg cggttgaact
     6481 gattctttgc cgtctgcatg caggcggtaa attctctaac aaaaattacc agttctctgg
     6541 cggcctgcat ggcgtgggga tttcggtggt taacgccctg tcgaagcgcg tagaagttaa
     6601 cgtacgccgc gatggtcagg tttataacat cgcctttgaa aatggcgaaa aggtgcagga
     6661 tttacaggtt gtcggcactt gcggtaagcg caataccggt accagcgtgc acttctggcc
     6721 ggatgaaacc ttctttgaca gcccgcgttt ttctgtttca cgcctgacgc atgtgctgaa
     6781 ggccaaagca gtactgtgcc ctggtgttga gatcaccttt aaagatgaga tcaacaacac
     6841 cgaacagcgc tggtgctatc aggacggtct gaatgattac ctggcggaag cggtaaacgg
     6901 tttaccgacg ctgccagaaa aaccgtttat cggtaatttc gctggcgata ctgaagcggt
     6961 ggactgggcg ctactgtggc taccggaagg cggtgaactg ctgaccgaaa gctacgtcaa
     7021 cctgatccca acgatgcagg gcggtaccca tgttaatggc ctgcgtcagg gcctgctgga
     7081 cgcgatgcgt gaattctgtg aataccgcaa cattctgccg cgcggtgtaa agctgtcggc
     7141 ggaagatatc tgggatcgct gcgcctatgt gctgtcagta aaaatgcagg atccgcagtt
     7201 tgccgggcag accaaagaac gtctctcttc gcgtcagtgt gcggcatttg tttctggcgt
     7261 ggtgaaagat gccttcacct tgtggctgaa ccagaacgtt caggcagctg aactgctggc
     7321 ggagatggcg atttccagcg cccagcgtcg tatgcgtgcg gctaaaaaag tggtgcgtaa
     7381 aaagctaacc agcggcccgg cgctgcctgg taaattggct gactgtaccg cgcaggatct
     7441 taaccgtacc gaactattcc ttgtggaagg tgactccgca ggcggatctg ccaagcaggc
     7501 gcgcgatcgc gaatatcagg cgatcatgcc gctgaaaggt aagatcctta acacctggga
     7561 agtctcttcc gacgaagtgc tggcttcgca ggaagtgcac gatatttcgg tagcgatcgg
     7621 tatcgatcct gacagtgacg atctgagcca acttcgttac ggcaagatct gtatcctggc
     7681 ggatgctgac tccgatggtc tgcacattgc cacgctgctc tgcgctttgt ttgtaaaaca
     7741 cttccgcgca ttggtgaaac acggtcacgt ttacgttgca ctgccaccgc tctaccgtat
     7801 tgacctcggg aaagaggttt attacgcgct gacggaagaa gagaaagagg gcgtacttga
     7861 gcaattaaaa cgcaagaaag gcaagccaaa cgtccagcgt tttaaaggtc tcggggaaat
     7921 gaacccgatg caattgcgcg aaaccacgct tgatccgaac actcgccgtc tggtgcagtt
     7981 gactatcgat gatgaagacg atcagcgtac tgacgcgatg atggatatgc tgctggcgaa
     8041 gaaacgctcg gaagatcgcc gcaactggtt gcaagaaaaa ggcgacatgg cggagattga
     8101 ggtctgataa tcttaacccg gcaaacatgt gttttgccgg gttgtcgttg attattttat
     8161 tgggcacagt ttattacgat ctggaaatag tgttgccagc gaaccagcca gagttttcca
     8221 gtccgtagcc attttttctg cttgtgcagt tgttattact gcaccttgtt gtttaataag
     8281 ctcatcctcc actctggtat agtgaataat ggtaaaatcc gtagaggacc atttatcctt
     8341 agagaacatc ttcgtcatcg catgaatgtt tgcttgcccg tcatcgataa aaacaatcgc
     8401 gtcatatttg ttccccattt tatccagcat ataatcgaga attacgcctt tatcttgccc
     8461 ggaagaaaaa aataccccct ggctgtacag ccatgaccgt ttgagtttcc ctttttcaaa
     8521 gggtggcaaa gtatcctctt tcttcctgag tgcggaagac gaaaaatcaa tgccgtttcg
     8581 ctttaattcc cgcaatgtcg gaaagagagt atccggtgca cgggatgtca gagcaaaagc
     8641 ggtatgcttt tgttgccact gttttaacat tgtcggtaac tgtggctcgg taagctgcat
     8701 agggctaagt tcgtaaagca tactgattgt attttcatac aggcaaggaa ctttttgcga
     8761 gtcatccggc tttaaaggca atttatcggt ttgccattga taccagatat caccgccaat
     8821 gcgagtgcca ctcgttaata acgtgttatc aatgtcaaaa accaataata tcttatcgga
     8881 tggatagaga gtttcctttt ctgtgataat tttactgacc gcactatatt cgccagtaac
     8941 tatatttttt acctgtgcgt aagataaagc aggcagagca gagagcatca tccagagaat
     9001 gattttaaat ttattattca ttttttattc cagattagaa agtataagtc agaccccagc
     9061 gccagcggat ttggcgatta cttgatgttt tatccacacc tttataatcc gcaatactca
     9121 tatagggtat ccacgatccc attttgtacc ctactttaaa tgatgcggta tagtttttat
     9181 ggccatcatt ccaggttaaa taaccattat tgtaatagac caggtttaac tgatattgga
     9241 agttggtttt ttgtggtcga tagtcgatcc acacatcgta acggcggtta ttacgatcat
     9301 cgttgccata acctttggat ttatcggtgg ctttatccat gtcataacga aaccgacctg
     9361 aaactttcca ttctttgctg aagtcataac ccgcagaaag accatattcg taagttgacc
     9421 aatattgcgt ggtatcaagc cagaacatgg gttctaacca gaattttttg tcatccagcg
     9481 gcttaaagcg gtaaacaaca gataatcctg ttccgttatt gccaaattgt gaggggtccc
     9541 acttatcttt atcaccttca tcggtgttat agcgtaattc tcccatcaaa ccgataccat
     9601 tactaaaaaa tgtaccaaat tcaacgcggt ccgcgtgggt gcgagtatca tcaagatact
     9661 catggcgata atcaataaat ccatgcggct gttctgcaca gactagtgta ggtgacaggc
     9721 aggataacat tgttattcct gcaagtgata ttttaagata agaattcata atattccctt
     9781 tcagaataaa atatattttt ggtgatcgaa tacaccatct gatgattcc
//
LOCUS       DAZUCC010000187         9747 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZUCC010000187 DAZUCC010000000
VERSION     DAZUCC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.188"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            445..2076
                     /gene="oppA"
                     /locus_tag="ACHY09_003995"
     CDS             445..2076
                     /gene="oppA"
                     /locus_tag="ACHY09_003995"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFS3886894.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIITNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2162..3082
                     /gene="oppB"
                     /locus_tag="ACHY09_003996"
     CDS             2162..3082
                     /gene="oppB"
                     /locus_tag="ACHY09_003996"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFS3886895.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3097..4005
                     /gene="oppC"
                     /locus_tag="ACHY09_003997"
     CDS             3097..4005
                     /gene="oppC"
                     /locus_tag="ACHY09_003997"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFS3886896.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4017..5030
                     /gene="oppD"
                     /locus_tag="ACHY09_003998"
     CDS             4017..5030
                     /gene="oppD"
                     /locus_tag="ACHY09_003998"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFS3886897.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNRLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5027..6031
                     /gene="oppF"
                     /locus_tag="ACHY09_003999"
     CDS             5027..6031
                     /gene="oppF"
                     /locus_tag="ACHY09_003999"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFS3886898.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6084..6413)
                     /locus_tag="ACHY09_004000"
     CDS             complement(6084..6413)
                     /locus_tag="ACHY09_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFS3886899.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6448..7908)
                     /gene="cls"
                     /locus_tag="ACHY09_004001"
     CDS             complement(6448..7908)
                     /gene="cls"
                     /locus_tag="ACHY09_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS3886900.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8051..8224
                     /locus_tag="ACHY09_004002"
     CDS             8051..8224
                     /locus_tag="ACHY09_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFS3886901.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8279..9532)
                     /gene="kch"
                     /locus_tag="ACHY09_004003"
     CDS             complement(8279..9532)
                     /gene="kch"
                     /locus_tag="ACHY09_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFS3886902.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2552 a         2285 c         2430 g         2480 t
ORIGIN      
        1 gaactgtaat cagaaaagag agacatgctt aaccaatctc tatttgattg aattgaaaga
       61 tgtttgttaa gacatggatg caagctatag attctgatgc ggtcaataaa agagaattgc
      121 ttaacaattt tgcaaaatgt attggcgagt aagaagcgca tttggtactt tccgggcaac
      181 cgccaggcga ttctttattg gtaatgagaa taattaacaa ttaaagagcg tcgcgaaaga
      241 ataatgtgtc tcgacagggg agacacagta cgaatcgaca taaggtgatc gtctgaatca
      301 ccagaataaa taaagtcggt gatagcaatc cgtaacgata aagtaacctg acagcagaaa
      361 gtctccgagc ctgtgcaggg tcccaatccg ggattacaca tgctggttaa taccagtaat
      421 tataatgagg gagtccaaaa aacaatgacc aacatcacca agagaagttt agtagcagct
      481 ggcgttctgg ctgcactaat ggcagggaat gtcgcgctgg cagctgatgt acccgcaggc
      541 gtcacactgg cggaaaaaca aacgctggta cgtaacaatg gttcagaagt tcagtcatta
      601 gatccgcata aaattgaagg tgttccggag tctaatatca gccgagacct gtttgaaggc
      661 ttactggtca gcgatcttga cggtcatccg gcacctggcg tcgctgaatc ctgggataat
      721 aaagacgcga aagtctggac cttccatttg cgtaaagatg cgaaatggtc tgatggcacg
      781 ccagtcacag cacaagactt tgtgtatagc tggcaacgtt ctgttgatcc aaacaccgct
      841 tctccgtatg ccagttatct gcaatatggg catatcgccg gtattgatga aattcttgaa
      901 gggaaaaaac cgattaccga tctcggcgtg aaagcaattg atgatcacac attagaagtc
      961 accttaagtg aacctgttcc gtacttctat aaattacttg ttcacccatc aacttcaccg
     1021 gtgccaaaag ccgctatcga gaaattcggc gaaaaatgga cccagcctgg taatatcatc
     1081 accaacggtg cctatacctt aaaagattgg gtcgtaaacg aacgaatcgt tcttgaacgc
     1141 agcccaacct actggaacaa cgcgaaaacc gttattaacc aggtaaccta tttgcctatt
     1201 gcttctgaag ttaccgatgt caaccgctac cgtagtggtg aaatcgacat gacgtataac
     1261 aacatgccga tcgaattgtt ccagaagctg aaaaaagaga tcccggacga agttcacgtt
     1321 gatccatacc tgtgcactta ttattacgaa attaacaacc agaaaccgcc attcaacgat
     1381 gtacgtgtgc gtaccgcact gaaactgggt atggaccgcg atatcattgt taataaagtg
     1441 aaagcgcagg gcgacatgcc cgcctatggt tacactccac cgtatactga tggcgcaaaa
     1501 ttgactcagc cggaatggtt tggctggagc caggaaaaac gtaacgaaga agcgaaaaaa
     1561 ctgctggctg aagcgggtta taccgcagac aaaccgttga ccatcaacct gttgtataac
     1621 acctccgatc tgcataaaaa gctggcgatt gctgcctctt cattgtggaa gaaaaacatt
     1681 ggtgtaaacg tcaaactggt taaccaggag tggaaaacgt tcctcgacac ccgtcaccag
     1741 ggcacttttg atgtggcccg tgcaggctgg tgtgcagact acaacgaacc aacttccttc
     1801 ctgaacacca tgctgtcgaa cagctcaatg aataccgcgc attataagag cccggccttt
     1861 gacagcatta tggcggaaac gctgaaagcg actgacgagg cgcagcgcac agctctgtac
     1921 actaaagcag aacaacagct ggataaggat tcggccattg ttcctgttta ttactacgtg
     1981 aatgcgcgtc tggtgaaacc gtgggttggt ggctataccg gcaaagatcc gctggataat
     2041 acctacaccc ggaatatgta cattgtgaag cactaatggc aatacgtggg gcaggcgtgt
     2101 actgctccac ggtgtctgat ttttatcgca ttacagaagg cacaggccag aaggtagggc
     2161 aatgttaaaa tttattctac gtcgctgtct ggaagcgatt ccgacgctat ttattcttat
     2221 tactatttcg ttcttcatga tgcgcctcgc gccgggaagc ccttttaccg gcgaacgtac
     2281 tttaccgcca gaagtgatgg ccaatatcga agcgaaatat catcttaatg atccgatcat
     2341 gacacagtat ttcagctacc tgaaacaact ggcgcatggc gatttcggtc catcgtttaa
     2401 atataaagat tattcggtca acgacctggt tgcatccagt tttcccgttt ctgccaaact
     2461 gggagccgca gcatttttcc ttgcggtaat actgggtgtt agtgctggcg ttattgccgc
     2521 attaaaacaa aacaccaaat gggactatac cgtgatgggg ctggcaatga ccggggttgt
     2581 tatccccagt tttgtggttg cgccattatt agtcatgata tttgcgatca ttttgcattg
     2641 gctgccgggc ggtggctgga atggtggtgc gcttaaattc atgatactgc caatggtggc
     2701 gttgtcactc gcctatatcg ccagtattgc gcgtattacc cgtggctcta tgattgaagt
     2761 attacactcc aactttattc gtactgcccg ggcgaaaggg ttacctatgc ggcggatcat
     2821 tttacgccac gcattaaaac ctgctctgtt acccgtgctc tcctatatgg gccccgcatt
     2881 tgtcggcatt attaccggtt ctatggtcat cgaaaccatt tatggtttgc cggggattgg
     2941 gcaactgttc gttaatggcg cattgaaccg tgactattcc ttagtgttaa gcctgactat
     3001 cctggttggt gcgttaacca ttttgtttaa tgccattgtc gatgtgctat atgcggttat
     3061 cgacccgaaa atccgttact gatactggag ctcgcgatga tgttaagtaa gaaaaacagc
     3121 gagacgctgg aaaatttcag tgaaaagctg gaggtcgaag ggcgcagctt gtggcaggac
     3181 gcacgtcgac gttttatgca taaccgtgcg gcggttgcca gtctgatagt gctggtgcta
     3241 atcgcgttat ttgtaatcct ggcaccgatg ctttcgcagt ttgcctatga cgatactgac
     3301 tgggcgatga tgtccagcgc cccggatatg gagtccggtc actactttgg tactgactca
     3361 tccggtcgcg acctgcttgt gcgtgttgcg attggcgggc gtatctcact catggtcggt
     3421 gttgctgcgg cactggtggc agtggtcgtg gggacacttt atggttcgct ttccggttat
     3481 ctgggcggta aagtggattc ggtaatgatg cgtctgctgg aaatcctcaa ctccttccca
     3541 ttcatgttct tcgtcatttt gctggtgacc tttttcggtc aaaacatcct gctgattttc
     3601 gtggcgattg gcatggtttc ctggctggat atggctcgta ttgtgcgtgg gcaaaccctg
     3661 agtctgaagc gcaaagagtt tattgaggcg gcacaagttg gcggtgtatc gacgtcgggc
     3721 attgttattc gccacattgt gccgaatgta ctcggtgtgg tggtggtcta cgcatcactt
     3781 ctggtgccca gcatgatcct ctttgaatct ttccttagct tcctggggtt ggggacgcaa
     3841 gagccgttaa gcagctgggg cgcattgctg agtgatggcg cgaactcgat ggaagtctct
     3901 ccatggttac tgttgttccc agcgggattc ctcgtggtga cgctattttg tttcaacttt
     3961 ataggcgatg gcttgcgtga tgccctcgac ccgaaagatc gttaaggagt gcagccatga
     4021 gcgtaattga aactgcaact gtgccgctcg cacaacaaca ggctgacgca ctgctgaacg
     4081 tgaaagattt gcgtgtcacc tttagtaccc cggacggcga cgtcacggcg gtcaatgatt
     4141 tgaatttttc cctacgtgcc ggagaaacgc tgggcattgt aggtgagtct ggttcgggta
     4201 aatcgcaaac tgcatttgcg ttgatgggcc tgctggctgc caacggacgt attggcggat
     4261 cggcaacctt caatgggcgt gaaatcctca atttgccaga gcgtgaactc aatagactgc
     4321 gcgctgaaca aatctcaatg atttttcagg acccaatgac ttcgttgaat ccctatatgc
     4381 gcgtcggtga gcagttgatg gaagtgctga tgctgcataa gaacatgagc aaagctgagg
     4441 cgtttgaaga gtcggtgcgg atgctcgatg cggtaaaaat gccggaagca cgtaaacgca
     4501 tgaaaatgta cccgcatgaa ttttctggtg gcatgcgtca gcgagtcatg attgcgatgg
     4561 cattgctatg tcgacctaag ctgctgattg cggatgaacc cactactgcg ctggacgtca
     4621 ccgtacaggc gcagatcatg acgctattaa atgaactgaa gcgggaattt aataccgcca
     4681 tcattatgat cacccacgat cttggcgtgg tggcggggat ctgcgacaaa gtgctggtaa
     4741 tgtacgccgg gcgcacgatg gaatatggca acgcgcgcga tgtcttttat caacccgttc
     4801 atccttattc tatcggtttg ctcaacgcgg tgccgcgtct cgatgcggaa ggtgaaacaa
     4861 tgttgaccat ccctggtaat ccgccaaacc tgctgcgatt accgaaaggt tgcccgttcc
     4921 agccacgttg tccgcatgcg atggaaattt gtagtagcgc accgccgctg gaagagttta
     4981 cgcctggccg tctgcgtgct tgctttaaac cggtggagga actgttatga atgctgtaac
     5041 cgaaggaaga aaagtcctcc tcgaaatcgc cgatctgaaa gtgcactttg aaatcaaaga
     5101 tggcaaacag tggttctggc aaccgccgaa aacgctcaaa gccgtcgatg gtgtcactct
     5161 tcgcctgtat gaaggggaaa cattaggtgt ggtaggggaa tcgggatgcg gtaagtccac
     5221 ctttgctcgc gccatcattg gtttggtcaa ggcgaccgac ggtcatgttg cctggttagg
     5281 taaagagttg ctgggcatga agcccgatga atggcgtgcc gttcgcagtg atattcagat
     5341 gattttccag gatccattgg catcgcttaa cccgcgtatg accatcggcg agatcatcgc
     5401 tgaaccactg cgtacttatc atccgaaaat gtcacgccag gaagttcgcg agcgcgtgaa
     5461 ggcgatgatg ctgaaagtcg ggttattgcc taacctgatt aaccgctatc cgcatgagtt
     5521 ctctggtggg cagtgccagc gtatcgggat tgctcgtgct cttattcttg aaccgaagct
     5581 gattatctgt gatgagccgg tgtcggcgct ggacgtgtca attcaggcgc aggtggtcaa
     5641 cctgctccag cagctgcaac gcgagatggg attgtcatta atttttatcg ctcatgacct
     5701 ggccgtggta aaacacattt ccgatcgtgt gctggtgatg tatctcggcc atgcggtaga
     5761 actggggacc tatgatgagg tctatcacaa tccgctacat ccttacacca gggcattgat
     5821 gtcggcagtc cccatacctg atccggatct ggagaagaac aaaaccatcc agttactgga
     5881 aggggaatta ccgtcgccga tcaacccgcc ttccggttgt gttttccgta cccgttgccc
     5941 gattgccggt ccagagtgtg ccaaaacacg tcctgtgctg gaggggagtt tcagacacgc
     6001 cgtttcttgc ctgaaagtcg atccgcttta aaagcaataa gggctgacag ttgtcagccc
     6061 ttattcacgc caaaagcgat tatttattcc cgccagatga tatggcacag tttgtgatct
     6121 ttttcgcggc ataacagaat gcgcgcgaaa acatcgttga tttcaccatc ttcactgtcc
     6181 gccagaccaa tcaccacttc ggcaaaaaag tccgggttca aatcgaaatc aacatgttcc
     6241 tgccagtctt ctgccggatc gaataactcc gcgccgccgc gctcttcaaa ctgaagattg
     6301 aacagcagaa catcagctgg atcgagattg tcggcagcca gttcgagaaa aatatcgtag
     6361 gcctgctcaa gcgtttcatc ttcagtcagg cgattgttta gatccatatc catagtcact
     6421 acctgtttaa cctctgttgg cgacgtttta cagcaacggg ctgaagaagt aaaacagtcg
     6481 ctcggcgaca cgttgccata atggacgttt tagccataaa cgggcatcga gcagacgtga
     6541 acgcgaaata taatcgtcct gaacggcggc aaggtctgca ccaaaacctt tatcgtcgat
     6601 tgccagggta atctcgaaat ttagccacag actccgcata tcaaggttaa cggtgccaac
     6661 cagacttagt tcaccatcga ccagcacgct cttggtatgc agtaacccgc cttcaaactg
     6721 ataaatttta accccagcag ccagcagttc cgtaaagaat gcgcgactgg cccagccgac
     6781 cagcatcgag tcattttttc gcggaaggat aatactgaca tccaccccgc gctgcgccgc
     6841 cgtgcaaatc gcatgaagta aatcatcgct gggcacaaag tagggcgttg tcatgatcaa
     6901 atattcacgc gccgaataag cagcagtcaa taatgcctgg tgaatgagat cttccggaaa
     6961 gccggggcca gaagcaattg tgtgaatggt gtgaccgctg gcctgctcaa acggcataat
     7021 attgacatct ggtggtggcg gcagaatacg ttttccggtt tcaatctccc agtcgcagga
     7081 ataaataatc cccatcgcgg tagcgatggg accttccata cgcgccatca gatcaatcca
     7141 ttgccctacg cccgcatctt gtttgaagta gcgagggtcg accatattca tgctgccggt
     7201 gtacgcgatg taattatcga tcatgatcat cttgcgatgt tggcgcaggt ccatacggcg
     7261 taaaaacaca cgcatcagat tgacctttaa ggcttcgaca acttcaatac cggcattacg
     7321 cattagctcg ggccacgggc tgcggaaaaa agccacactc ccggcggagt cgagcatcaa
     7381 tcggcaatga atgccgcgtc gcgcagccgc cattaatgat tcagccacct ggtccgccat
     7441 gccgccgggc tgccagatat aaaacaccat ctcaatatta tggcgagcga gctggatgtc
     7501 gcggattaac gcctgcatca catcatctga ctcggtcatc agttgcagct gattcccttt
     7561 gaccccagcg atcccctgac gacgctcgca aagcttgaat aatggcgcag cgacactgct
     7621 attttcttcg gcgaagatat gcttacaggc tttaaggtcg ttaagccatt ttgcggtgga
     7681 aggccacatc gctctggcgc gctcagcgcg gcgtttgcct aaatggagct cgccaacggc
     7741 aagataggca ataattccga ctaacggcag aatgtaaata atcaacagcc aggccatcgc
     7801 ggagggaact gcgcgtcgtt tcattagaat gcgtaaagtt acgcctgcaa tgagcaacca
     7861 gtatcccaga atggccaacc aactcaccaa cgtataaacg gttgtcatag attagaaatc
     7921 cttttgaaag cgcattgtta tgagtttacg catatggatt catctggcaa ataaaaacgc
     7981 gggaaaagca ctggtcagcg tcgggggcgg gggtataatg accattctgt tattgcatag
     8041 agtagttaac atgaagcgga gtagaacgga agtggggcgc tggcgcatgc agcgtcaggc
     8101 tagccgacgt aaatcgcgtt ggcttgaggg gcagtcgcgc cgaaatatgc gtatccacag
     8161 catcaggaag tgcattctaa acaaacagcg taactcgtta ttgtttgcga tctacaatat
     8221 ctaaatgtac tgggcaccat tgcggtgccc aataagttta ctcttccctc tttaaactct
     8281 atttttgcgc cgattcttta ctgtcagctt tcgtttcttg ttcatcgtta tcgctgaaaa
     8341 tgccatgacc ggagttcaac aacattgaaa cgagcatatc attattaatc tcttcaccat
     8401 ttaaaactcg cgccagaatt tcgctgccaa acagttgcgg cgagagaatg atatccggat
     8461 gtaccatctt aatcttattc aggtttttgc tatcactgac ggcgagaact gttttgacat
     8521 cactgctcat atcttttgcc gagagtacaa caaacgcgtt atctgcatcg ttatcactca
     8581 gcgccagaat ggcccggcat cgatcaattc cagctttctt taatactgaa ctgtcattac
     8641 tgtcaccggg gataacatca gcgttatcgc ctaagcgttg ctcaagttgc ttgatatcat
     8701 cttcaggcaa gttgctgata accgttacgt tttgtccgcg ttgattcagt tgcaggatcg
     8761 tattgatggc gagaatcgaa tgtccgcaaa caataaaatg atctttacga tgcattgtat
     8821 gattgtttcc ttttacaagt ttgttgaatc ccccgcggat aagcggacca aaaattgagg
     8881 tcatagatgt ggcaaaaacg gtaatgccgg aaataatgac cgaaatagtg aacaatcgtg
     8941 ctgattcaga aacagggaca atatcgccat agccgacggt tgacatggtt tctatcgaaa
     9001 aatagaacgc ggtcatcaaa ctttctattc gcggattaaa accttcgctt aaataaagcg
     9061 caccgtaggt ggagtaaaac agtaacgtcg tgaaactaat aaatgcgaaa attgtcccgg
     9121 ctgcggcgct actgtgggag aagtctttgc gcagtatcag caaaaacacc agcgtaaaaa
     9181 tgcaaaatcc aatactaaat ttcagccagg gataaaaatg cagggtgtaa attagcgcta
     9241 tcaacaacaa aatgatactg attgcccatg cgagctttgc ccggaacagt aagccaatgg
     9301 agttcaggac aagaaagacg ccgagcataa agagtggggc gtttgccagc aaggagagat
     9361 ccaactctga aaatgctttg atatggaaaa tatctagcaa attgaccgac ataccataga
     9421 ttgttttaaa aataagcaat ccatttaaaa agacggccag cgcgagaatg tcgtgccgta
     9481 gggtcaccca taaattagtt gctgtttgtt tgaatgtagc ccagtgactc actgatacct
     9541 ctgtaaccac aattttgttt aattatctta gcgtgcacat tttctgaaca tacatgcagc
     9601 gcgtgaatgt gttaaacaat agtagatgac tttatccgca gtaatcaaag gttttgagtg
     9661 agcaactctg cgtcaggaaa gggcattttt cctgaatatg ctcacatcat ataaagaaat
     9721 acagataaag ttattatctg cttgtgg
//
LOCUS       DAZUCC010000188         9740 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZUCC010000188 DAZUCC010000000
VERSION     DAZUCC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.189"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(175..1209)
                     /locus_tag="ACHY09_004004"
     CDS             complement(175..1209)
                     /locus_tag="ACHY09_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS3886903.1"
                     /translation="MSKKKGKTPRPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTPFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDND"
     gene            complement(1209..2981)
                     /locus_tag="ACHY09_004005"
     CDS             complement(1209..2981)
                     /locus_tag="ACHY09_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFS3886904.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            3155..4009
                     /locus_tag="ACHY09_004006"
     CDS             3155..4009
                     /locus_tag="ACHY09_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFS3886905.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHDAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            4068..5141
                     /locus_tag="ACHY09_004007"
     CDS             4068..5141
                     /locus_tag="ACHY09_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFS3886906.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLVADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            5145..5888
                     /locus_tag="ACHY09_004008"
     CDS             5145..5888
                     /locus_tag="ACHY09_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFS3886907.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDIRLLTETLELTATAD
                     MPDEVRAKLHKITGLFLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRVPRKTRSVTPAKRGRPKKKAS"
     gene            5988..6497
                     /locus_tag="ACHY09_004009"
     CDS             5988..6497
                     /locus_tag="ACHY09_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFS3886908.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            6497..6700
                     /locus_tag="ACHY09_004010"
     CDS             6497..6700
                     /locus_tag="ACHY09_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFS3886909.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            6704..6985
                     /locus_tag="ACHY09_004011"
     CDS             6704..6985
                     /locus_tag="ACHY09_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS3886910.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            6985..7482
                     /locus_tag="ACHY09_004012"
     CDS             6985..7482
                     /locus_tag="ACHY09_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFS3886911.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            7497..7922
                     /locus_tag="ACHY09_004013"
     CDS             7497..7922
                     /locus_tag="ACHY09_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFS3886912.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVATWAILATVIC
                     VVAGGVGVYATEYVLELYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRKACRQRLEGRELCQS"
     gene            7910..8335
                     /gene="lysB"
                     /locus_tag="ACHY09_004014"
     CDS             7910..8335
                     /gene="lysB"
                     /locus_tag="ACHY09_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFS3886913.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     TTITMLKNQLHVAITRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCPQRLPESESLPDAGQ"
     gene            8235..8480
                     /gene="lysC"
                     /locus_tag="ACHY09_004015"
     CDS             8235..8480
                     /gene="lysC"
                     /locus_tag="ACHY09_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFS3886914.1"
                     /translation="MLCAGCTSARPAQTPVIVHNACPKVSLCPMPGSDPQKNGDLSAD
                     IRLLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            8443..8910
                     /locus_tag="ACHY09_004016"
     CDS             8443..8910
                     /locus_tag="ACHY09_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3886915.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSMSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            8903..9355
                     /locus_tag="ACHY09_004017"
     CDS             8903..9355
                     /locus_tag="ACHY09_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFS3886916.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSTELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEETILAHLER"
     gene            complement(9427..>9740)
                     /locus_tag="ACHY09_004018"
     CDS             complement(9427..>9740)
                     /locus_tag="ACHY09_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886917.1"
                     /translation="LSFKNKTVELNKTYSLADCKTLQNVFIKDTLPEIACNKLLQLNE
                     EDSEWLSQEIKDNNSHKKALLILSLVYFISGLLIFLSYTKFLYANKKVLEYKASNKNH
                     S"
BASE COUNT         2152 a         2587 c         2853 g         2148 t
ORIGIN      
        1 agcactgcgc gctggcggtg gtgctgattt tattttttca gcgtctgagc gcgtcgtgac
       61 ggcgcttagt ctgcccgttg aggcgttggt gtgtctgcgg ggtgttttgt gcggtggtga
      121 gcgtgtgagg gcgtgatgac ggggtgtaaa aaagccgccc gcaggcggcg atgttcagtc
      181 gttgtcagtg tccagtgagt agtttttaaa gcggatgacc tcctgaccga gccagccgtt
      241 tatctcgcgg atcctgtcct gtaacgggat aagctcattg cggacaaaga cctttgccac
      301 tttctcaata tcacccagcg acccgacgtt ctccggcttg ccgcccatca actgaaaggg
      361 gatgcggtgc gcgtccagca ggtcagcggc gctggctttt ttgatattaa aaaaatcgtc
      421 cttcgttgcc acttcactga gcgggataat tttaatgccg tcggctttcc cctgtggggc
      481 atagagaaac agatttttaa agttattgcg gcctttcgac ttcaccatgt tttcgcgaag
      541 catttcgata tcgttgcgat cctgcacggc atcggtgacg tacatgatgt atccggcatg
      601 cgcgccgttt tcgtaatact tgcggcggaa cagcgtggcc gactcattca gccaggcaga
      661 gttaagggcg ctgagatatt ccggcaggcc gtacagctcc tgattaatat ccggctccag
      721 caggtgaaac acggagccgg gcgcgaaagg tgtcggctcg ttgaaggacg gcacccacca
      781 gtaaacatcc tcctcaacac cacggcgggt atattttgcc ggtgatgttt ccagtctgat
      841 gaccttaccg gtggtgctgt agcgcttttc cagaaacgca ttaccgaaca ccagaaaatc
      901 cagcacaaag cggctgaaat cctgttgcga aagccacgga tgcgggataa atgttgaggc
      961 cagaatattg cgtttgacgt aaatcggtga gctgtgatgc acggcagcac gcaggctttt
     1021 tgccagaccg gtaaagctga ccggtggctc ataccatctg ccgttactga tgcactcgac
     1081 gtaatccaga atgtcacggc ggtcgagtac cggcaccggc tcaccaaagg tgaatgcctc
     1141 cattttcggg gcgctggcgg tcattgtttt tgccgctggt cgcggtgttt tccctttttt
     1201 cttgctcatc agtaaaactc cagaatggtg gatgtcagcg gggtgctgat accggcggtg
     1261 agtggctcat ttaacagggc gtgcatggtc gcccaggcga ggtcggcgtg gctggcttcc
     1321 tcgctgcggc tggcctcata ggtggcgctg cgtccgctgc tggtcatggt cttgcggata
     1381 gccataaacg agctggtgat gtcggtggcg ctgacgtcat attccagaca gccacggcgg
     1441 atgacgtctt ttgccttgag caccattgcg gttttcattt ccggcgtgta gcggatgtcg
     1501 cgtgcggcgg gatagaacga gcgcacgagc tggaacacgc cgacaccgag gccggtggca
     1561 tcaataccga tgtattcgac gttgtatttc tcggtgagtt tgcggatgga ttcagcctga
     1621 gtggcaaagt ccatgccttt ccactggtga cgctcaagta ttctgaattt gccaccggcc
     1681 accaccggcg gtgccagtac cacgcatccg gcgctgtcgc cacggtgtga cgggtcgtaa
     1741 ccaatccata ccgggcggga gccgaacgga ttcgcggcaa acggcgcata gtcttcccat
     1801 tcttccagcg tgtcgaccat gcagcgttgc agctcctcga acgggaacac cgacgccttg
     1861 tcgtcaacaa attcacacat gaacaggttt ttaaaatcgt cggcgctgtt ttcgcgtttg
     1921 agctgctcaa tgtcgaacag cgtgcagccg cctttcaggg cgtcctcaat ggtgacaatc
     1981 tgccgccact ggccgtccgc acagagaagt ccaccggcaa gggcgttatg actgacgtcg
     2041 atttccacgc gttcggcggc gctggcgcgt ccccggttaa acagttcacc cgaccagaac
     2101 gggtaggcgt cgtgcgccag cgtggacggg gtggagaaat aggtcgaacg caggtgactc
     2161 tgtgaggcca tacctgatgc caccttacgc agtacctgaa aattcgggat ccagaaaatc
     2221 tcgtcgacgt acaggtcgcc gttatggctc tgtgcggtgt tggagttggt gccgagaaaa
     2281 atcagttttg cgccgttatt gcccaggaca atcgggtcac cggtcaggtc aacgtcaacc
     2341 agccgggcaa aggcgatgat gtattcgcgg aacacatacg cctgcgtttt actggccgac
     2401 agaaaaatct ggttatgacc ggttttcagg gcgcgcagca gcgcctcgcg ggaaaaataa
     2461 aacgtcgcgc caatctggcg ggatttcagg atatcgcgga tgcggtgctc aagcccggcg
     2521 cgataccagt gcaactgata gtcgaaagac tgctcaaaga aaatctgctc cagcttttcg
     2581 atggcttcgt cactgaaaaa attctttttc ggtttgcgac gcccaccttt gttgcggtta
     2641 gcaatgttcg gattaaggtc tgcctcgttg ccggtctggc tgtaacggtt gacccgtgcc
     2701 agtcgttcaa tctggcgtcc cagcaggtca atttccttga agtccccgcc ggttttctgc
     2761 ggtttgatga tgagctgggt cagccgcgct tccagactca tttcgacacg gctgatgggg
     2821 gcaacgctgt cccagccgtc gcgctgtttc cagctctgca ccgtcgggcg tttcatctgc
     2881 aacatggcgg caatctgcgg cacggaaaat ccctgccagt acagcagcgc cgcctgacga
     2941 cgcgggtcgt gtaaaagagt ggtgtctgtg gtgatggtca tgaatacctc gccgtgataa
     3001 atacacggca aggctactga gtcgcgtccc gcgattcgct aaggtgctgt tgtgtcagtg
     3061 ataagccatc cgggactgat ggcggaggat gcgcatcgtc gggaaactga tgccgacatg
     3121 tgactcctct aatcactatt caggactcct gacaatggca aaaaaagtct caaaattctt
     3181 tcgtatcggc gttgagggtg acacctgtga cgggcgtgtc atcagtgcgc aggatattca
     3241 ggaaatggcc gaaacctttg acccgcgtgt ctatggttgc cgtattaacc tggaacatct
     3301 gcgcggcatc ctgcctgacg gtatttttaa acgttatggt gatgtggtcg aactgaaggc
     3361 cgaaaagatt gacgatgatt cggcgctgaa aggcaaatgg gcgctgtttg cgaaaatcac
     3421 cccgaccgat gaccttatcg cgatgaacaa ggccgcgcag aaggtctata cctcaatgga
     3481 aattcagccg aactttgcca acaccggcaa atgttatctg gtggggctgg ccgtcaccga
     3541 tgacccggca agcctcggca cggaatacct ggaattctgc cgcacggcaa aacacaaccc
     3601 tctgaaccgc ttcaaattaa gccctgaaaa cctgatttca gtggcaacgc ctgttgagct
     3661 ggaatttgaa gacctgcctg aaaccgtgtt caccgccctg accgaaaagg tgaagtccat
     3721 ttttggccgc aaacaggcca gcgatgatgc ccgtctgaat gacgtgcatg acgcggtgac
     3781 cgctgttgct gaacatgtgc aggaaaaact gagcgccact gagcagcgcc tcgctgagat
     3841 ggaaaccgcc ttttccgcac ttaagcagga ggtgactgac agggcggatg aaaccagcca
     3901 ggcattcacc cgcctgaaaa acagtctcga ccacaccgaa agtctgaccc agcagcgccg
     3961 cagcaaggcc accggtggtg gcggtgacgc cctgatgacg aactgctgac cggcgtcagt
     4021 cagtccggga aaaccttcac gattaaccct taatttcagg aaaaactatg cgccaggaaa
     4081 cccgctttaa atttaatgcc tacctgtccc gtgttgccga actgaacggc atcgacgccg
     4141 gtgatgtgtc gaaaaaattc accgttgaac cgtcggtcac ccagaccctg atgaacacca
     4201 tgcaggagtc ctctgacttt ctgacccgca tcaacattgt gccggtcagc gaaatgaaag
     4261 gggaaaaaat tggcatcggt gtcaccggct ccattgccag caccaccgac accgccggtg
     4321 gcaccgagcg tcagccgaag gacttctcga agctggcgtc caacaagtac gaatgcgacc
     4381 agattaactt cgatttttat atccgctaca aaacgctgga cctgtgggcg cgttatcagg
     4441 atttccagct ccgtatccgt aacgccatta tcaaacgcca gtcccttgat ttcatcatgg
     4501 ccggttttaa cggcgtgaag cgtgccgaaa cctctgaccg cagcagcaat ccgatgctgc
     4561 aggatgtggc agtcggctgg ctgcagaaat accgcaatga agctccggcg cgcgtgatga
     4621 gcaaggtcac tgacgaggaa gggcacacca cctctgaggt catccgcgtg ggcaagggcg
     4681 gtgattatgc cagccttgac gcactggtga tggatgcgac caataacctg attgagccgt
     4741 ggtatcagga agaccctgac cttgtggtga ttgtgggacg tcagttactg gcggacaagt
     4801 atttccccat cgttaacaag gagcaggaca acagcgaaat gctggtcgct gacgtcatca
     4861 tcagccagaa acgcatcggt aacctgccgg cggtacgcgt cccgtacttc ccggcggatg
     4921 cgatgctcat cacgaagctg gaaaacctgt ccatctacta catggatgac agccatcgcc
     4981 gcgtgattgt ggaaaacccg aaactcgacc gcgtggagaa ctacgagtca atgaacattg
     5041 attacgtagt ggaagactac gccgccggtt gtctggtgga aaaaattaag gtcggtgatt
     5101 tctccacacc ggctaaggcg accgcagagc cgggagcgta accgatgacg agtcccgcac
     5161 agcgccacat gatgcgggtc tcggcagcga tgaccgcgca gcgggaagcc gccccgctgc
     5221 gacatgcaac tgtctatgag cagatgctgg ttaagctggc cgcagaccag cgcacactga
     5281 aagcgattta ttcaaaagag cttaaggccg cgaaaaagcg cgagctgctg ccgttctggt
     5341 tgccgtgggt gaacggtgtg ctggagcagg gcaaaggtgc acaggatgac attctgatga
     5401 cggtcatgct gtggcgtctg gataccggcg atattgccgg tgcgctggag attgcccgtt
     5461 atgccctgaa gtacggtctg accatgccgg gtaaacaccg ccgcaccccg ccgtacatgt
     5521 tcaccgagga ggtcgcactc gcggccatgc gcgctcacgc tgccggtgaa tctgtggata
     5581 tccgcctgct gacggagacc cttgaactga ccgccacggc tgacatgcct gatgaagtgc
     5641 gcgcaaagct gcacaaaatc accggtctgt ttctgcgtga tggtggtgat gccgccggtg
     5701 cgctggctca cctgcaacgt gcgacacagc tcgactgtca ggcaggcgtc aaaaaagaga
     5761 ttgaacgact ggagcgggag ctgaaaccga agccggagcc gcagcccaaa gcggccaccc
     5821 gtgtcccgcg taagacccgg agcgtgacac cggcaaaacg tggacgcccg aaaaagaaag
     5881 ccagttaaca accgaatgcg ccccgcgcca gggcggcacg ccggtcagtg acggtgaatt
     5941 acctgacact gcaccggcgt ccaccgcccg acttttcaga ggtagtcatg atgacgctga
     6001 ttattccgcg aaaggaggct cccgtgtccg gtgagggtac ggtggtcatc ccgcaaccgg
     6061 caggcgacga gccggtgatt aaaaacacgt tcttttttcc cgatatcgac ccgaagcgcg
     6121 tccgggaacg tatgcgcctt gagcagaccg tcgcccccgc ccgtctgcgt gaggccatca
     6181 agtcaggcat ggctgaaacg aatgcggagc tgtacgagta ccgcgaacag aaaattgccg
     6241 ccggttttac gcgtctggcg gacgttccgg cggacgacat cgacggtgaa agcatcaaag
     6301 ttttttacta cgagcgcgcc gtgtgtgcga tggcgaccgc gtcgctttat gagcgttatc
     6361 gcggcgtgga tgccagtgcg aaaggcgaca agaaggccga cagcattgac agcaccattg
     6421 atgaactgtg gcgggatatg cgctgggcgg tggcgcgtat ccaggacaag ccgcgctgca
     6481 tcgtgagtca aatctgatga agacctttgc gctacagggc gacacgctcg acgccatttg
     6541 tgtccggtat tacgggcgca ctgagggcgt ggttgagacc gtgctcgccg caaatccggg
     6601 actggctgaa ctgggtgcgg tgctgccaca cggcaccgcc gtcgaactgc ccgacgttca
     6661 gaccgcgccc gtggctgaaa ctgtcaatct gtgggagtaa cgcatgacag cagaagaaaa
     6721 aagcgtcctg tcgcttttca tgattggggt gctgattgtt gtcggcaagg tgcttgccgg
     6781 tggtgaaccc atcaccccgc gtctgtttat cgggcgcatg ttgctcggcg gttttgtctc
     6841 gatggttgcc ggtgttgttc tggtgcagtt tcctgacctg tcactgcctg cggtgtgcgg
     6901 catcggttcc atgctgggta tcgccggtta tcaggtgatt gagattgcca ttcagcgccg
     6961 ctttaagggc agggggaaac cgtaatgccg gtaattaaca cgcatcagaa tatcgccgcc
     7021 tttctcgaca tgctggccgt gtccgaaggg acggcgaatc atccgctgac gaaaaaccgg
     7081 ggctatgacg tgatagtcac cggactggac ggaaagccgg aaattttcac cgactacagt
     7141 gatcacccgt tcgcgcatgg ccgaccggcg aaggtgttta accgtcgcgg tgaaaaatcc
     7201 acggcctccg gtcgctatca gcagctttac ctgttctggc cgcactaccg caaacagctt
     7261 gccctgccgg atttcagtcc gttgtcacag gacaggctcg ccattcagtt gatccgcgaa
     7321 cgcggtgcac tggatgacat ccgggcggga cgcattgagc gcgccatttc acgctgtcgc
     7381 aatatctggg cgtccctgcc gggtgccggt tacggtcagc gtgagcattc actggaaaaa
     7441 ctggtcaccg tctggcgtac cgccggcggc gtaccggctt aaacggagta aacaccatga
     7501 agaaattatc cctttcactg atgctgaacg tgtcgctggc gctgatgctg gcactgtccc
     7561 tgatttaccc gcagagcgtg gccgtcagtt ttgtcgccac ctgggcgatt ctggcgacgg
     7621 ttatctgtgt ggttgccggt ggtgtcggcg tgtatgccac ggagtatgtg ctggaactct
     7681 acgggcggga gctgccgccg gaatcgctgg ccgtgaagat tgtcacgtcg ctgtttttgc
     7741 agccggtgcc gtggcgcaga cgggcggcgg ctctggtggt gatggtggcg acgtttatct
     7801 cgctggtcgc tgccgggtgg atttttaccg cgctgattta tcttgtggcg tcgctgtttt
     7861 tccggctgat acgtaaagcc tgtcgtcagc gtcttgaggg gcgggaacta tgtcaaagct
     7921 gatgattgtg ctggtcgtgt tgttatcgct ggcggtggcc ggtctgtttc tggtgaaaca
     7981 caaaaatgcc agcctgcgcg cctcgctgga cagggcgaat aacgtcgcca gtgaacagca
     8041 gacgaccatc accatgctga aaaatcagct tcatgttgcc atcaccaggg cagacaaaaa
     8101 cgagctggcg caggtggcac tgcgtcagga actggagaac gccgcgaagc gtgaagcaca
     8161 gcgcgagaaa accatcacga ggttactcaa tgaaaacgaa gattttcgcc gctggtacgg
     8221 tgctgacctg cctgatgctg tgcgccggtt gcaccagcgc ccggcctgcg cagacgccag
     8281 tgattgtcca caacgcctgc ccgaaagtga gtctttgccc gatgccgggc agtgacccgc
     8341 agaagaacgg cgatttaagt gccgatatcc ggctgcttga gaacgcgctg gcacgctgtg
     8401 ccagccaggt aaaaatgatt aaacactgtc aggacgaaaa cgatgctcaa acccgacagc
     8461 ctgcgcaggg cgctgactga tgccgtcacg gtgctgaaaa ccagtcccga gatgctgcgg
     8521 atattcgtgg ataacgggag tattgcctcc acactggcga cgtctatgtc attcgaaaag
     8581 cgttacacgc tcaatgtgat tgtgaccgac tttaccggtg attttgacct gctcattgtg
     8641 ccggtgctgg cgtggctgcg ggaaaatcag cccgacatca tgaccaccga cgaaggccag
     8701 aaaaagggct tcacgtttta tgcagacatc aacaatgaca gcagctttga tatcagcatc
     8761 agcctgatgc tgaccgagcg cacgctggtc agtgaggttg acggcgcgct gcatgtgaag
     8821 aatatcccgg aacctccgcc gccggagccg gttacccgcc cgatggagct gtatatcaat
     8881 ggcgaactgg tgagcaagtg ggatgaatga gtttaagcgt tttgaagacc ggctgaccgg
     8941 actgattgag tcgctgtcac cgtcagggcg tcgacgactg agtaccgaac tggcgaaacg
     9001 tctgcgtcag agtcagcagc gtcgggtgat ggcacagaaa gccccggacg gcacacccta
     9061 cgcgccacgc cagcagcaga gcgccagaaa aaagaccggt cgtgttaagc gaaaaatgtt
     9121 tgcgaaactt atcaccagtc gctttttgca tatccgcgcc agcccggaac aggcatcaat
     9181 ggaattttac ggcgggaagt caccaaaaat cgccagcgtg catcagttcg gcctgtcgga
     9241 agaaaaccgg aaagacggta agaaaattga ttatccggcg cgtcccctgc tcggctttac
     9301 cggtgaggat gtgcagatga ttgaagagac tatcctggct caccttgagc gttagtttta
     9361 tccaggcaga ggctgatgcg caattaaaca ttgagcggcc gtgctggtcg ctcaatgttt
     9421 agaggtttat gagtggtttt tatttgatgc tttgtattct aaaaccttct tattggcgta
     9481 aaggaatttt gtatatgaca ggaatataag caggcctgaa atgaaataga cgagggatag
     9541 tattaataat gcttttttgt ggctgttatt atctttaatc tcctgactta accattcgga
     9601 gtcctcctcg tttagctgta agagcttatt gcaggcgatt tcaggaagtg tgtcttttat
     9661 aaatacgttt tgcagggtct tgcaatcggc aaggctataa gttttattaa gttcaactgt
     9721 tttatttttg aaggataaaa
//
LOCUS       DAZUCC010000189         9463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZUCC010000189 DAZUCC010000000
VERSION     DAZUCC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.190"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            166..1230
                     /gene="ulaG"
                     /locus_tag="ACHY09_004019"
     CDS             166..1230
                     /gene="ulaG"
                     /locus_tag="ACHY09_004019"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS3886918.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1338..2093
                     /gene="ulaR"
                     /locus_tag="ACHY09_004020"
     CDS             1338..2093
                     /gene="ulaR"
                     /locus_tag="ACHY09_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFS3886919.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2090..2839)
                     /gene="yjfP"
                     /locus_tag="ACHY09_004021"
     CDS             complement(2090..2839)
                     /gene="yjfP"
                     /locus_tag="ACHY09_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS3886920.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHSTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLADRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3021..3350
                     /gene="bsmA"
                     /locus_tag="ACHY09_004022"
     CDS             3021..3350
                     /gene="bsmA"
                     /locus_tag="ACHY09_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFS3886921.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3499..3774
                     /gene="yjfN"
                     /locus_tag="ACHY09_004023"
     CDS             3499..3774
                     /gene="yjfN"
                     /locus_tag="ACHY09_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFS3886922.1"
                     /translation="MKQFLASSSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3882..5507)
                     /locus_tag="ACHY09_004024"
     CDS             complement(3882..5507)
                     /locus_tag="ACHY09_004024"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFS3886923.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERMEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5591..6754)
                     /locus_tag="ACHY09_004025"
     CDS             complement(5591..6754)
                     /locus_tag="ACHY09_004025"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFS3886924.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6757..7395)
                     /locus_tag="ACHY09_004026"
     CDS             complement(6757..7395)
                     /locus_tag="ACHY09_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFS3886925.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7405..7803)
                     /locus_tag="ACHY09_004027"
     CDS             complement(7405..7803)
                     /locus_tag="ACHY09_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFS3886926.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7821..8480)
                     /locus_tag="ACHY09_004028"
     CDS             complement(7821..8480)
                     /locus_tag="ACHY09_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFS3886927.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFLRALGVDIPLTSLHII
                     G"
     gene            complement(8530..9228)
                     /locus_tag="ACHY09_004029"
     CDS             complement(8530..9228)
                     /locus_tag="ACHY09_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFS3886928.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9247..>9463)
                     /locus_tag="ACHY09_004030"
     CDS             complement(9247..>9463)
                     /locus_tag="ACHY09_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFS3886929.1"
                     /translation="SNPDEFNTFLLRNQKMMPLSSVGISSVQHEEYYIVFGALSLKSS
                     LEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         2466 a         2520 c         2248 g         2229 t
ORIGIN      
        1 attgaattac acattccatt aaatctttcc acactcaaaa tcaacaacaa cttccctggc
       61 aaaaagtcaa aaaatggtca ttgccttcct gaaaaaatag tgtcaggatt aatcaaaacc
      121 aatcacatgt tgattcgaat tgaactatca tgaaggtaaa tggcgatgag taaagtgaaa
      181 agtatcaccc gagaatcctg gatcctgagc actttcccgg agtggggtag ctggctgaat
      241 gaagaaattg aacaagagca ggtcgcccct ggcacattcg cgatgtggtg gctaggctgc
      301 accgggatct ggctgaaatc ggaaggtggc accaacgttt gcgttgattt ctggtgcggt
      361 actggcaaac aaagtcacgg taacccgctg atgaaacagg gtcaccagat gcagcgcatg
      421 gctggcgtga aaaaactgca gccaaacctg cgcaccaccc cgtttgttct tgatccgttt
      481 gcgattcgcc agatcgacgc ggtactggca actcacgatc acaacgatca tatcgacgtc
      541 aacgtcgctg ctgccgtgat gcagaactgt gctgatgacg taccgtttat cggaccgaaa
      601 acctgcgtgg atttatggat tggctggggc gtaccgaaag agcgttgcat cgtggtcaaa
      661 ccgggcgatg tcgtaaaagt gaaagacatt gaaattcatg cccttgacgc gttcgaccgt
      721 actgcactga tcaccctacc agcggatcaa aaagcggctg gcgtgctgcc agacggcatg
      781 gacgatcgcg cagtaaacta tctgttcaaa acaccgggcg gttctctgta tcacagcggc
      841 gattcccact actctaacta ctatgcaaaa catggcaacg agcatcagat cgacgtggct
      901 ttaggttcat acggcgaaaa cccacgcggt attaccgaca aaatgaccag tgctgatatc
      961 ttgcgtatgg gcgaagcgct gaatgctaaa gtagtgatcc cgttccacca cgatatctgg
     1021 tcaaacttcc aggccgatcc gcaagagatc cgcgtgctgt gggagatgaa aaaagatcgc
     1081 ctgaagtatg gcttcaagcc gtttatctgg caggtcggcg gcaaatttac ctggccgctg
     1141 gataaagaca acttcgagta ccactatccg cgtggtttcg atgattgctt cactattgaa
     1201 ccggatctgc cgttcaagtc gttcctgtaa tctgtcgctt gtaccggata gcgtggagta
     1261 gttcgcgcta tccggcgtag tatttcctgg ctatttcaaa tatcatctaa aaaaatcaaa
     1321 ttttatcgga atagctcatg actgaagcac aaagacatca aatcctcctg gaaatgctcg
     1381 cacaattggg ctttgtgacc gttgagaaag tcgttgagcg tctgggaatt tcgcctgcca
     1441 ctgcgcgacg cgatatcaat aaacttgacg aaagcggcaa actgaaaaaa gtgcgcaatg
     1501 gcgcagaagc tattacccag cagcgcccac gctggacgcc gatgaatctg catcaggcgc
     1561 agaatcacga tgaaaaagta cgtattgcta aagcggcctc gcagctggtt aatccgggcg
     1621 aaagcgtagt catcaactgc ggctccaccg cgtttctgct tgggcgggaa atgtgtggca
     1681 agccagtgca aatcatcact aattatctac cgctggcaaa ttacctgatc gatcaggagc
     1741 atgacagcgt gatcattatg ggcggacagt acaacaaaag tcagtccatc actttaagcc
     1801 cgcaaggcag cgaaaacagt ctctatgccg ggcactggat gtttaccagc ggcaaagggc
     1861 tgacagcaga agggttgtat aaaaccgata tgctgacggc aatggcagag cagaagatgc
     1921 taagcgtggt agggaaactg gtggtactgg ttgatagcag taagatcggc gaacgcgcgg
     1981 gaatgctttt tagccgtgcc gatcaaatcg atatgctgat caccggcaaa aatgctaacc
     2041 cggaaatcct gcaacaactg gaagcgcaag gggtcagcat tctgcgtgtt taaagatgct
     2101 ggcggaaaaa tgtcacggca gcatctaacg cctcaggcgt aatgcggtga cgcacgcctg
     2161 gctgccatga acaggttagc agtttatccc gtcccgtctc gcttaaggcc tgctgcaaac
     2221 gtagtgattc gtcggcaggc acgacgtcat cgtcaaggcc atgccacagc agtagaggtc
     2281 tgtcagcaag ttgttccagg tggtttgtcg cttcccactc tgccagcggc gcgacaatgt
     2341 tattgaattc attctgctgt gctgccgttt caggtatcag cggtggaaac agtgaacggg
     2401 cgagcgatgt aaaatagccc gatcccatca tgctggcggt acatttcacc gtggagtggc
     2461 gagcggtaat tcccagtgcc gtcatcgcgc ccatcgacgc gccaccgact gccagacggt
     2521 catcacgcag ccagttttct tcggctattg ccgcacgtaa agtagtgaat tcctgcatac
     2581 tttgtagcaa gatttgccag aattgattta accgccgcgc tgcgtcgcca ctaaaacgac
     2641 taccgtgatc gggcgcatcc ggcatgatca cccgcaaacc agcctgcgcc agcgcaacgg
     2701 caaaatagct atacaccaga ctggacgaag taaagccgtg ataaaaaatc gcgcacggta
     2761 acggggtatc tttttgcccg acaggataag catgaagaac gggaatatct gccagctcgc
     2821 gtgattctat ttcaatcatc tgtctctcct ttttgtctgg tatgacatga agaaatcctt
     2881 agcacaagtt tctgctggcg gtattagttc ataatgttga gatgtgggtt acgctttcat
     2941 cacgttttca ttcgaaaatc gcgtaccagg taacaattcg ggaacattcc cccaaagtta
     3001 aattaataag gcactacact atggttagca ggaaacgtaa tagcgttatt taccggtttg
     3061 ccagtttatt attggtgttg atgttaagtg cctgtagcgc actacaaggt acgccacagc
     3121 cagcaccgcc agtaaccgac catccgcaag agattcgccg cgaccagacg caagggttac
     3181 agcgaatagg tagcgtaagc acgatggttc ggggttctcc ggatgacgca ttagcagaaa
     3241 tcagagcgaa agctgtcgct gcaaaagctg attattacgt tgtcgtcatg gtggacgaaa
     3301 ccatcgtcac aggacagtgg tattcacaag ccattttgta ccgtaaataa atcagacaac
     3361 ttacatagtc agctttacat tgctttgcgt ccctatgcgt ttacctgtgc acaataaatt
     3421 accggctgga agcctgggcg tttattaccg cgatggaatg ccctgcaaca tgtggggaaa
     3481 cgaaaaatgg agctgacgat gaaacaattc cttgcctcat cctcgctgca attagtgact
     3541 tatcctgcga gcgccacggc acagtctgca gaattcgcca gtgctgactg cgtgacaggc
     3601 ctgaatgaaa taggtcagat ctcagttagc aatatttcag gggatccgca ggacgtggaa
     3661 cgtatcgtgg cgttaaaggc tgatgaacaa ggtgcgtcat ggtaccgcat tattaccatg
     3721 tacgaagacc agcagcctga taactggcgc gtacaggcga tcctgtatgc gtgagtgtca
     3781 tctacagcaa atgaacaatg ccggatgcgg cgcgagcgcc ttgtccggct aacaattttc
     3841 acatatttca ttgagttaat cgaaaccagc gtcgtatacg cttacaaaca cactcctccc
     3901 gtcgcccgca gcaataaatc attctggatc tgctctgaca accgtacgcc gccgcgcgta
     3961 tctaacatca cctgacacca cgcctgcgcc attggcggag aggcatattt caacatttgc
     4021 gcaccgcagc ccagcaggaa tagctgatga gtaatctctc gccccagttc ttcagctggc
     4081 ttacgcagct gctgctgtaa acgacgaacc gcgcgatcaa aatagcgatc ctgccctttc
     4141 acttccacaa atgcttccga caataagtcg tatacacccg cttgcttatt gagcacgcgc
     4201 agtacatcca ggcacataat attaccggaa ccttcccaaa tactgtttac cggcatctcc
     4261 cggtaaagcc gcggcagctc gctctcctcg caataaccaa tgccgcccag cacctccatc
     4321 gcttcggcca caaacggcat acctcgtttg cagatcacaa atttcgccgc aggcgtaaat
     4381 aaacgcgccc acagggcttc tttggcatcg gcacgccggt cccacgctcg agcaagacga
     4441 aacagcaacg ccgtttgccc ttcaagctga agtgccatgc gacttaagac atgacgcata
     4501 aggggctgtt ggatcaatgg attaccaaaa acatggcgtt gatgtgcatg ataaatcgct
     4561 agcgaaaatg cacggcgcat catggcatgg ctacccaggg cgcaatcaaa acgcgtcatc
     4621 ccacccattt tcaggatcag acgaattcct tccccttcct gccccaacaa ccagccaatg
     4681 gcatcctgaa actccacttc gcagctggcg ttagagcgat tacccagctt atctttcagc
     4741 cgctcgaggc gaatcgcgtt gcgttgcccg tcaggcaaaa agcgcggcac aaaaaagcag
     4801 gacaaaccgc ccgtagtctg cgccagcacc agatgcgcat cgctttgcgg caccgagaaa
     4861 aaccatttat gccccaccag ccgataagag ccatcttcca tgcgctctgc gcgggtggtg
     4921 ttgctcatga catcggaacc gccctgtttt tccgtcattc ccatgccaat caacaaaccg
     4981 cgtttttgcc cacctggcaa taagtgagaa tcgtagcgat cgctcaacag cggcgtggtc
     5041 cagtcctgaa acggcgcggg taacatctgt aacagcaatg gcgtggcggc aaaggtcatc
     5101 gttatcggac ataacgaccc tgcctcaacc tgtgcgtgca acataaaacg cgccgcgcgc
     5161 gccacaaatg cgccggagcg agcgtcttct tcccaggcaa gattgtgcac ccgattggta
     5221 catagcgcct gcatcagcag gtgccaggcg gggtgaaaac gcacatcgtc cagacgccgc
     5281 ccctgggcat cgtagcgcaa taattcaggc ggattcacat tcgccagccg ccccagttca
     5341 agggattcag ccgtccctaa ctgctgacca atactggcga gaaaatcgct atcccagcca
     5401 gcaccttcac gcgttaccgc ttcgcagagc gcgccatcag acaggtataa gttgctgtta
     5461 tttaatggta taggttgatt aaaaacggtg tgagtttgcc agtgcactgt gtctccctcc
     5521 atcaatggca gtcaccatta gtatggtcac tgccatggat tcatgacaga aagagattgc
     5581 taaaacattc ttatccagca atgtaatgcg gaacgaaacg tgaggtgtct ttggtgatca
     5641 acgtgttatc ttcacggatc cccattccgc acgcttcatc atcgacaatc cagctaccga
     5701 tgagtgtgta gctatcgcca aatcgcggca gaggttgaaa cgcctggtag atcatcggtt
     5761 catcggcgta atcaccatca gcgtggtcaa caacgttatt ctgaccgtcg aaaatggtga
     5821 cgtttccgcc ttcgcgcgag taaatcggtt tacgcacata gctttcgcca gcggcaatct
     5881 gcggtttttc accatcgaac caggatgcaa gaaggttagg atgaccaggg aagaagcgcc
     5941 aaagcagcgg cattagccct ttattactca agatactttt ccataacggc tccacccatt
     6001 gctcgcgacg cttgcgcagc agcggaccgt tatcgtcacg catcatccac tccagcggat
     6061 acagcttaaa tgcacgctgg atgacattat catcaagatc ggtcagcaca ccgccgacgc
     6121 ccaaaccgag atcttcaatg tagataaacc gcgactcctg ccctgcctgc tcggcgcagt
     6181 cctgcaagta cagcacggta ctcctgtctt cgtcggtgtc ctgacagcag caaaaataaa
     6241 acggttcccg actgtaaagc tcgctaaagc gtgaaatcag gcgctcctga atggcattgt
     6301 actgatcggc atcgcgcgga ataacgccgc tgcgccgggc atcttccagc cacaaccact
     6361 ggaaataagc cgactcgtac aatgaagttg gcgtatcggc gttgtactcc agcaacttaa
     6421 ccggtgcatt accacaccag gcaaaatcca tacggccata caacgaagga tcgcgtgcgc
     6481 gccagctttc ggcgatcaca tcccagtaca acggcgggat cgccagttgc gtcaggatct
     6541 cttcgtcttt aaccgcgcga tccaccacct caaggcacat ctgatgcagt tccgcagtcg
     6601 gtttttcgat ctgctcttca atctggcgca gagtaaaacg gtaagctcga ctctcgtccc
     6661 aatagatttc attgtcgatg atatgaaagt cgaaaccgtt gtcagcggcg atctggtcca
     6721 gatcccgccg cacaggaacg ttgtgtctca gcatgattag cctccccaat gcccacgggc
     6781 gctggaagaa cgaccatagc cgccgcgaga aacggttgac gcttttttgg tggtaaagcc
     6841 gcccgaagag taagactctt ttttgccaga accggagcgc caggagtaat caccagaagt
     6901 gttgcgccag acggggcgcg aagcaaatga ggatccgccg ctgttataga cgaacggctc
     6961 atcgcgatct ttacgaataa cccggcttaa caaaaatcca gaaacgaccg ggatccaact
     7021 ctgttcaaca ttgtcgtagt agcaattttc gtacttagac tgacaattct gctgagtcat
     7081 attcttcgga acatcggcat aaaacgccgc tttggcgttg ttccagccac gcgcgcaaat
     7141 gtctgcatta ttaccgtcat caatacaatc ctgcacagtc gcgtaaaacg ttccgtcgcc
     7201 gtcgttatcg acatcgccac tatcgccaca acctttcaat acgaaaaaag cggcaccgcc
     7261 cattagcgcc aacgttaagt atttttgtgc gtagcgattg cggcgcggct caaaaggatt
     7321 atgcggtcta ccaatgcgac tgattgcccc atgtccgata tttttgttat ttctggattt
     7381 gcgttttctg gccatgatcg tccattacca tgtcatacaa gctgcgttaa aaataccgcc
     7441 agccagcgcg gcggttccca taaacattcc tgctgcggta ttgtgattaa taattttttc
     7501 gcttaatgcg ggcatataaa gcctgacgcc agcaaaaacg agtaattgaa tcaccagagc
     7561 aatcccgccc catgcgaaat agtctggaat actcaccgca ttgattgccg cactggataa
     7621 ggggatcacg taccccaaca atgttccgct gaacgccagt gccgccgcgg tgttattatt
     7681 tttgattaac tgccattcgt tgtgcggtgt aattttggag tagataaaca gaaaaataat
     7741 caccatagcc acaccaataa aaaaataagc gctaaaggca agaagtgaat ccagtatatg
     7801 catcgtaaac gtccttgtaa ttaaccaata atatgaagtg atgtcagcgg gatatcgacc
     7861 cccagcgcgc gcaaaaataa ccactccggc tcgccgagat cattaaaaga ttcctcacca
     7921 tttaacagca gatattcgta agtatcttcg gtcacttgcc gttgatagcc catggtaaaa
     7981 ttatggacct cccatttagc atggttttga ttttctactt tttccagcat gtagacgggt
     8041 tcgatatttc ccggttcttc gctattaaaa aatcgctgcc agcgtttttc ctgccagttt
     8101 aaggtcattg cccccatcgc tttggcattg atcgcctcgc gccagtgact ctctttactg
     8161 ataccgtagc tctcttcata gacaaagagc ttgatatcat caatgtcatc aatatcttcg
     8221 ccgccagtgg tattgatttg cagaaactca tcgcctgaag tgtagtagcg gaaaatctgg
     8281 ctaccgccgc ccagatcgat gcggctgaca gcggctaccg taaattcctc acccggcagc
     8341 gcaatcagca attcatcttc cagcaaacga aacgctaacg tgtcgagcgt aaagccacta
     8401 ttgagatgaa gtcccagagg accgcgagcg atagcaggct tattatcctt gccaaacaga
     8461 cgctggaaaa aaccagacat attttatcct taaaatagtt gccagccttg cagtgaggct
     8521 ggcaaaaaat tactcgccct gttggcgttg cagtcttgct aatacatcct gggcgctact
     8581 tttattgcta ccgccaattc cggcttccgc cagcttttca tcaagatcgc gaccgtctgc
     8641 gactttttcc aactgtgcgg cagcatccag gcgagcctga cgttcggcct gacgcgtttg
     8701 caggcgtttt aaggattctg ccgctgtcga aacgctggaa gatgcgccaa cagtagaggt
     8761 cgttacagcc tgttgtgcac gctgcatggc ttcagtagct ttaacgactt ccatttgctg
     8821 ctcaaactga gcgatacgct gcgctgtcgc tgtaactgct ttttccacgc catcacgaga
     8881 gacttccaga ttcgacaaca cttgctcttc agcggtaatg agattctcaa ggcgcgcgat
     8941 ttcttcagca acttcgttaa tcaacgacgg attaacgttc ttgcttaacg cttccagcgc
     9001 acgagcttcc agactggctt tgcgctcacg taaatctttc agcttatcgt gacttaattt
     9061 cacccgcgcc agcagatcaa cgcgagattt tccggcttta tcgagttcag ctttagcgtc
     9121 acgaatatgc tgttccagca tgcgcacgcc ctgggtttct tcaatggatt cttccgcctg
     9181 ggagataaac gatttcccca gcgtaaataa actttttaaa attcccataa atacccgtcc
     9241 ttgtaattag tgtgaatatt cttctgtaat ttcagccaga tccaatgcgt tatctaccag
     9301 cgaggttatc tcaagcagga tatcttcaag agaagattta agcgataacg caccgaaaac
     9361 aatgtaatac tcttcatgtt gcacgctgga gatccctacc gatgacaacg gcatcatctt
     9421 ctgatttctt aataagaagg tattaaattc atctggattg ctg
//
LOCUS       DAZUCC010000190         9424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZUCC010000190 DAZUCC010000000
VERSION     DAZUCC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.191"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(414..2018)
                     /locus_tag="ACHY09_004031"
     CDS             complement(414..2018)
                     /locus_tag="ACHY09_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFS3886930.1"
                     /translation="MNPDYVQALQTSPHFPTDVTYRLFPSDLAYLIDDLYSSTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMTPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKAVNRGDPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALAEGLNEHPEAGIISDEAVTFFRSYLKNNPGLLNKAWSGQ
                     PFDFGRADEKYHITPRLTFSLMSQPNVFTDYINKNDVLAWGSGFLSRFLFSQVGGSSR
                     VRDYRKGEFVTIPTLVEFHKKINGFLSSHSINSPGMRSERKTLKLSKKALGEWQENQI
                     KIESKALAGGEWEHIRDIVLKAGSNILRIAGLFTCYCYKDDEEITSIALWKAMHLMEW
                     YLEEASTIFYPMSERCQFEQDARELYTWIMTRIKQNNLYAIRKTDIERYGPNRLRRAE
                     KLTPVLNQLIAQNYLCIIKMRSHQALYVSVHCNNRYLLPCGAMSYEQFDIVPPLNSHN
                     AKTYHVNIPLALIPSFALPFSAYTLF"
     gene            complement(2448..3353)
                     /locus_tag="ACHY09_004032"
     CDS             complement(2448..3353)
                     /locus_tag="ACHY09_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3886931.1"
                     /translation="MALEQEIANLVNDFVAGGRPSCRDQTFDERRAGYIASTALAGDT
                     ETRVNISDVIIDDILLRIFSPLNDDKLSSAIVYYHGGCFVSGGFVTHDNQFRQLAFLS
                     GCRVIAVQYRLAPEHIYPAAHDDAEHAANIVWQHAEMLKVDRNKITLMGDSAGGHLAL
                     ITALRVKNIAQWLPRKLILIYPMLDATASCDSYKLNGSDYVLTEDTFLSGYEAYFSGL
                     DLEHPEASPLFRKDFNGLPPVHIITAEYDPLRDEGEELFSRMIQQGVKCTAQRYLGVI
                     HGFFQLGGISQTARNLMQDICGIVE"
     gene            complement(3807..4259)
                     /locus_tag="ACHY09_004033"
     CDS             complement(3807..4259)
                     /locus_tag="ACHY09_004033"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFS3886932.1"
                     /translation="MNVIDSGIRDVKFGENVKVVMPVNLYECELGDDVFIGPFVEIQK
                     GCIIGNRSRIQSHSFICENVILGDGCFIGHGVTFANDLFKCGGPDASAESWIRIVLGN
                     TVTVGSGATILTTKICSGAVIGAGSVVTKPVLVKGVYAGNPARLLRTL"
     gene            complement(4773..5504)
                     /locus_tag="ACHY09_004034"
     CDS             complement(4773..5504)
                     /locus_tag="ACHY09_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFS3886933.1"
                     /translation="MNQSIHNAYWLSRFDSLLDSAPAQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDIWCSLGDFSEPSSLATMIQQAWCSALHPEPWQGDGLVHFSRWTPSRKPA
                     AADASPSSDDTPLHSGCSDTRTDSDKRPGGSAVLWAKRGDVEAMQKALERARYLVKNE
                     TKLHDGSGQRNYGCSRGVRCLLDGR"
     gene            6175..6705
                     /locus_tag="ACHY09_004035"
     CDS             6175..6705
                     /locus_tag="ACHY09_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3886934.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAAMTITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
     gene            8385..9188
                     /locus_tag="ACHY09_004036"
     CDS             8385..9188
                     /locus_tag="ACHY09_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886935.1"
                     /translation="MSADGISDLIKPEIFLVIFPGTTTTAKRGGAGWLNNKRVVFRVW
                     LYISKASYKKMGIFQRGSAPESIPYREYHFPSYPDTHEKRKKVLRDWISFMRSRFNIN
                     QIAQFCYRMENEWAFTFDNIHVPRHFREGTDDTEWSWNYIKKKRLFSLSGLQHIQPQN
                     NTERKLLINAFFDTCLIENNPADDIDIKNMLLRKMNAAFYKRKSRARARIHSRNNQQM
                     SVSSGTDKQVNIRLKDFQYQQLLFIAQKENKSKSEIIKELITRAYRAIQ"
BASE COUNT         2868 a         2140 c         1831 g         2585 t
ORIGIN      
        1 gttattcaga aaaagatcga catcgtgata tacgccagca ctcatcacga caacaatcaa
       61 acaaagttat atataaaaac tcacataata tggttaataa ttagcttcaa tccatgtgaa
      121 aaataacatt tcgattcact ttcgagatat caaaaacgaa aacaattctt ccaccagtgc
      181 acacattcct gcattcaacc agctctgaca acaggtcatt atgaaaacaa aatcaaatca
      241 catacatggt acatgtactg ctaccgttgc taccgtagcg attttttcag agaaattttc
      301 gttcacaccc cataattggt gaatatttgc acaaatgcag gatatgaaaa acgccttacc
      361 atcaaccttc gaatgaaggc ttatgtgttt tgtttgctta ttgtaaagct gtttcaaaat
      421 aaggtatagg ctgaaaaagg taacgcaaat gacggaatta atgccagagg gatattaacg
      481 tgatatgttt tcgcattatg actattcaag ggtggaacaa tatcaaactg ttcgtaagac
      541 attgcaccgc aaggaaggag gtaacgattg ttacaatgaa cagaaacata aagtgcctga
      601 tgcgatcgca ttttgatgat acataaataa ttctgtgcga ttaactgatt gagtacaggt
      661 gtaagttttt ctgctcttct cagacgattg gggccatatc tctcaatgtc tgttttcctg
      721 atagcataca aattattctg cttgattcgg gtcataatcc atgtatacag ttcacgggca
      781 tcctgttcaa actggcatcg ttcagacata ggataaaata ttgtgcttgc ctcctccaga
      841 taccactcca tgagatgcat cgctttccaa agcgctattg atgtaatttc ctcatcgtct
      901 ttatagcaat agcaggtgaa taaacccgct atcctcagta tattagaacc tgctttcaga
      961 acaatatctc tgatgtgttc ccattcccca cctgcaagcg ctttgctttc aattttaatc
     1021 tggttttcct gccactctcc caacgctttc ttcgaaagtt ttaatgtctt cctttcgctt
     1081 ctcattccgg gggaattaat gctatggctt gacagaaacc cgttaatctt tttatgaaac
     1141 tccaccaggg ttggtattgt tacgaactcg cctttcctat aatcacgtac ccgggaagag
     1201 cctccaacct gactgaaaag aaaacgggaa agaaatccgc ttccccatgc cagtacatca
     1261 tttttattta tataatccgt aaaaacattc ggctgagaca ttaatgaaaa tgtcaggcgt
     1321 ggagtaatat gatatttttc gtctgctctc ccaaaatcaa acggttgtcc actccatgct
     1381 ttattcaaca gacccggatt atttttcaga tagcttctga aaaaagttac cgcttcatca
     1441 gaaataatcc ctgcctccgg atgttcgttg agtccttccg caagcgcttt aagcgaaaca
     1501 tcttcataga taaaattcgg tcgaaccgga cgtgtcggtt tatttctttc gtgattacgc
     1561 agtgcctctt cttcctgttc tcccggatcc ccccggttaa cagcctttct taaattggca
     1621 gcaagagctt tctgccgggt attccagatc tgtagttcag tcttataatc tttgtttctc
     1681 tcttcgtatt gttgaatgag tcgatcggca aattcgtaac agggggtcat caccagtctg
     1741 tttatcgttg tttttcccgc gcctggctct gcgattgcca acagataaag tgagcagggt
     1801 tccggcatgt tagtgtgagg atgaacaacg tcaaccaatg actgacagga gagtgagagc
     1861 gttgccagta cagtattaaa aatgagctcc agaggaagtt gggtacttga atacaagtcg
     1921 tcaatgagat acgcaagatc actaggaaat aatctgtagg ttacatctgt tgggaagtgg
     1981 ggggatgtct gcaaggcttg cacataatcg ggattcattg taatggacct ccttcacata
     2041 ctgtaatcag cacaaagctt attgttcagt tcagaaacat accaacattg tgagtatgtt
     2101 cgctgacgcc aataataaaa tgactgatct ccattcacca gtcccggcgc atttatggtt
     2161 ctgacaaaag taaacaacat gggagccaga actctgcatc gcagcgacaa actatggatg
     2221 catgaaaaac agggaacatt acgcaccaga gcgttgagag tcttcctcct tgcccaatac
     2281 gaaagtcatc tatccacccg tcttcaatct tcgggatggt tgttttatga acagagtaaa
     2341 agtttgaagc aagctgagaa cctctgtttc cacgtaaagg tccgctatga agaaaaatgg
     2401 gagtttgcat gctcaactaa tttacctgta ggtgcttgtt actgtagtta ttcaacaatc
     2461 ccacaaatat cctgcatcag attacgggct gtctggctaa tgccgccaag ctggaaaaaa
     2521 ccgtgaatga caccgaggta acgctgggcg gtacacttta ctccttgttg aatcatacgg
     2581 gaaaacagtt cctcaccttc atcgcgtaat gggtcatatt ccgctgtgat gatatgaaca
     2641 ggtggtaacc cattgaaatc ttttctgaat aatggactgg cttccggatg ttctaaatcc
     2701 agaccggaaa aataagcctc ataaccacta agaaacgtgt cttctgtcag cacataatca
     2761 cttccgttta atttgtaact atcgcatgat gctgttgcat ccagcatagg gtaaataagt
     2821 attaattttc gtggtaacca ctgagcgatg tttttcaccc gcagtgctgt tatcagtgca
     2881 agatgtcccc ctgcgctatc tcccattaac gttattttgt tgcgatcaac tttcaacatt
     2941 tctgcgtgtt gccatacaat attagcggca tgttcagcat cgtcatgtgc cgcaggataa
     3001 atatgttccg gcgctaatcg atattgtaca gctatgacac gacagccgct caggaaagca
     3061 agctgtcgaa actgattatc atgggttaca aaaccaccac tgacaaaaca gccaccatga
     3121 taatagacaa ttgcggatga caatttatca tcgtttaaag gcgaaaagat acgcaataaa
     3181 atatcatcaa taatcacatc actgatgttt acgcgggttt ctgtatcgcc ggccagagca
     3241 gtacttgcaa tatatcccgc ccgacgttca tcaaatgtct gatcacgaca ggatggacga
     3301 ccacccgcaa caaaatcatt tacaaggttt gcgatttcct gttcaagtgc catacaaatc
     3361 aactccgacc gataatattt aaacaacgct acgggaagta taaatactct gaccaaaata
     3421 taacaggtga atatccatag cttcaacagg cgtaatcaat aagggcgtac ggagcttgcc
     3481 cgttattgct gcaggatcga cagactggtt ttcgtccttg cgcatgtaac agtacaacca
     3541 cagagaaaag catcaatgga cactttatgt tgcgtcaggg cagcgaacga ctccttttgt
     3601 tgacgacaca tatcaccttc tgtactggca attacggcat tcagagtctc cctgagtttt
     3661 aagctcctgc tcttacggac cagcgacaag caataaataa ctatgacaat gaatctaata
     3721 acagcataaa tatgatactc atcgtgatta gtataatcgg ggcagaatta tattcactgt
     3781 ctgatttatt attgggttaa atataattaa agtgttctca aaagtctggc tggattaccg
     3841 gcatacacgc cttttaccag cacgggcttt gtaaccacac ttccagcacc aatcactgct
     3901 ccactgcaaa tttttgttgt taaaatagtc gctccgctac ccactgttac agtgttacct
     3961 aatacgatac gtatccagct ttctgcagag gcatcgggac caccacactt aaaaagatca
     4021 ttagcaaaag ttacgccatg gccgatgaaa cagccatcac ctaaaatgac gttctcacag
     4081 atgaaactgt gggactgaat tcggctccgg ttgccaatga tgcatccctt ctgtatttcc
     4141 acaaagggac caataaaaac atcatccccc agttcacatt catagaggtt tactggcata
     4201 acgactttca cattttcacc aaatttcaca tcacgaatgc cgctgtcaat aacgttcata
     4261 tccgttgtat cctccctcag gatgattctt atcagaacat ttcacctgtc agaatattcc
     4321 cggtaatgtg atacaagatc cagtctcacc gggtaccacg tgttattgcc cgtatccttt
     4381 tgcccgttac gagtctacaa ctggttttgt attttgcaat ccaggcctgt tacaccagag
     4441 catcctctca cttccccctc actggtcagt ccgggattca acatcatcct ccagcactaa
     4501 ccctatacca gtcctgaacc ttccctcgcc agcagcctac tgttcaccac aatcctcttc
     4561 cgtgaatggc agtaagtgag ctgcaccaaa ataaatcggt acttccgatg acagacagtg
     4621 gaagtgccag gagaaatatg aacatgagaa tggaagcaga taaaaatacc gttgcatgac
     4681 ttcaggacgg gttatcagaa tacgagctaa gggcgtcagc caacgccctt accgtggtat
     4741 acactcaggc accggatacc attttaccgg gttcacctgc catccagtag acacctcacc
     4801 ccacgactgc aaccataatt acgttgacct gaaccgtcat gcagcttcgt ttcatttttc
     4861 acgagataac gggctctctc cagggctttc tgcattgctt ccacatcacc acgcttggcc
     4921 cagagcacag cggatccacc cggcctttta tcagaatccg tccgggtatc agaacatcca
     4981 ctatgcaaag gcgtgtcatc ggaagaaggg gaagcatcag ccgctgctgg tttacgggaa
     5041 ggcgtccacc tggaaaaatg gaccaggcca tcgccttgcc agggctcagg atgcagagcg
     5101 ctgcaccatg cctgttggat catcgtcgcc agcgaagacg gttcgctgaa atccccgagc
     5161 gagcaccaga tatctttgtt gagcagtaat atcacgtgat aatgtttcct gcctttttcc
     5221 ttgccaaact cccgacacca gaaataacgc agggtggttg catgcacacg ctgattggca
     5281 catctttttt tccgctggta agcctgaatt ttggctttca gggatgcaaa aaaacgagaa
     5341 atcaccgctg aatccggatc gggatccatg atggtgacag gcatatactc agggaaacgt
     5401 aaatccaccc ggattaacga gacggcacgg tgctgcgccg gggcactgtc gagaaggctg
     5461 tcaaaacggg ataaccagta agcattgtgt attgattgat tcatgatgag agatctccgt
     5521 tgatttggat tgacttcaca tcattcatca acgcggtgaa actttacggc atcccgccaa
     5581 tgagcaacgc caatagctcc tgattaaaac ggaatgaatc gtcccgtaac gaaaagataa
     5641 tcctgcacac gacagcaaaa cgtaaaacaa cagcatgaat gagctaaatg tttaaagaaa
     5701 aaatgtcttt tcacattatt tttcagcaag taatcatacc agcatcccta acatacgaaa
     5761 tcacatagtg atcgatcgct gattcgctta acaactgatc gaatacaccg gaatttacac
     5821 taacatcctg atgggtaacg gaagactaaa aaacctgtgt tctattgatt gcacggaatg
     5881 cctgtgcact ggcctgacca gtaagcacaa ttctacgagt agtaagatat ctacgccaga
     5941 accatgcggc agtagccacc atatgttgcc agggtgaaaa ataagctcag agctataggg
     6001 gagtaattcc cgttaatgtc atcgctgtac ctcaatcagt tcacaaactg gctgaaacgt
     6061 taagctgaag cctttattct tcagatgtat tttccggttt acctgtaaat tatcttctga
     6121 tatttatcac attagtagtt gtgctgatta tctacatact tcaaggtctg aaatatgaag
     6181 ctaacagaag aaagtctgaa tctagtcatt gacatcctga atgatttgat ctgtgaagga
     6241 aaaatgctga acgatgtgga gcgcagatct ctgctcaatg cagctatgac aattacagcg
     6301 gtaaaagaac gttctgtcgt atctgtatca tcccgaaaag agcaaagtaa gaaggagaag
     6361 aaggaaagag agatcgatcc tcgtttccca aatgctggtg tggcatggaa tgaggcagac
     6421 gagagtttat tgcgagatgt tctggaggat gtaccggacg atgaaattgg taaacacctt
     6481 ttctggctgg cagagaaact agggcgaacc ccgtacagtg tagcctgtaa agtcagacaa
     6541 attaagaaat tacctgcaga atggaaggat caatttcgta aaatctctga caggatccgt
     6601 agttctggaa tgtcagtcag tgaatatcta caacgccagg gactgttaag cccagagtca
     6661 ggggaagaaa acaatcctgt cagtcataac aacaatgtag cttaatcctg aagaagatga
     6721 acagtttata ctctttaaac tgttccctta attttagaaa aacagtttgt gtaagtgcat
     6781 gctccaacat tctgaaggag ttatttctta caatacatca taatcattgg cagatcctca
     6841 tctgaatcaa aacaccacaa ccaatcatca cactttttct gaaaagagcc ttatgatgaa
     6901 gataatcacc ttacgctgag aaaaaacacc agggccagtt aatcacctta tcaatctcgc
     6961 cgttacaaaa tcatccataa gccatttaac agtgttatcg caaactcaca gtaaatcgca
     7021 tcaggttacc tcaatcggat ttgagttaat tcactgggct ttacgtaaag atacttttct
     7081 caggttaacg actaaaacac gaaaactcag aaaaggttaa acacaggttc agtaaaatcg
     7141 aatatcatct tgattgctta aacaggacag agggttttat agtgaggtta actgatttta
     7201 gcgatcgtaa aaaaaggact aacaaattta ctaaactgga tgtgtatcag acaaagcaat
     7261 gtgtatagca atgcttaatg aaaatacttg tatgattgtc tatgaaagta cgtgtatata
     7321 gtatcatacc gacattctca actcttccaa acatccatgc agactgaatg ttggtttcag
     7381 tttttcacgg ccagcatggg gatttttgtt ctgaaaacgg agcagattta ctctggaatt
     7441 attttccctg tcgggatatt tttcacctga aaactttcta caacacaacg gctgtataac
     7501 atcattctgg catattcatt caggtgagtc ggacaagcga taatcctgtt ctttaccgaa
     7561 cgaacaggca gcgataaaaa actgattcag acgacatttc tgtctgacaa acattcacat
     7621 gcagaagacc gggacattta ttcttcccga aaggaagtga taccttttct tatcatggta
     7681 gctgcattac ctgatatctt tcaggcgaat cccctccctg taaatctgat cgtcagataa
     7741 aagagatgaa catcaggcag atttccgtca ataacgataa gagatgacca tcgtaaacat
     7801 atcttccagt aacgaactgg cacctgcata ttaccgttta ttctcataca tcattgaaat
     7861 attgtcagtt ataaaggaca cacatcagga aattatcacc atgtgacaac gtcacagatg
     7921 gtgctgatat ataaaaatta acaaaatcgc gccaaaaaac cgcgccgata ttgcgcatct
     7981 gtaatccatc atttcctttt tgaataacct tccgtggtta cccgacatcc tgataatgac
     8041 gcccatcgta cacgcttttc tttgctcaga catcgcatac ttttatcctg ctcaataccg
     8101 tcttcctgcc agtcttccca ctctctgaca ctcacagaac agcaacgtcc tccggacata
     8161 ctagcagggc agaacacaat ccacaacatt aagtatatgt aacaataata taccattaag
     8221 accacttcta tgaccttata tgttacaacc ttagagcatg tatttaatat cccataatac
     8281 agactcgtca ttgttcttct gagacgacat gggcatgtca caaaactaat tgactgacat
     8341 atcatgaaaa tgtcagtatt taataattta atggaggtac aaacatgagc gcagatggca
     8401 tttccgattt aataaaacca gaaatatttc ttgtcatttt tcctggtaca acaaccacag
     8461 caaaacgggg tggcgccggg tggttgaata ataaaagagt cgtgttccgg gtatggcttt
     8521 atatcagtaa agccagttat aagaagatgg ggatttttca gcgcggttct gcgcccgaat
     8581 caatacccta tcgtgaatac cattttccct cataccctga cacccatgaa aaaaggaaaa
     8641 aggtattacg tgactggata tccttcatgc gctcccgttt taatataaat cagatagctc
     8701 agttctgtta tcgtatggaa aatgaatggg cattcacctt tgataatatc cacgttccac
     8761 gacactttcg ggagggaact gatgacacag aatggtcatg gaattacatt aagaaaaaac
     8821 gattattttc cctgagcggc ctgcaacaca ttcagcctca gaacaatact gaacgaaagc
     8881 ttctcattaa tgcttttttt gatacctgcc ttattgaaaa caaccctgct gacgatattg
     8941 acataaagaa catgttactc agaaagatga atgcagcgtt ttataaaaga aagagccggg
     9001 cgcgtgcgcg aattcactcc cggaataacc aacagatgtc agtcagttca ggaacagata
     9061 aacaggtaaa tattcgtctt aaggattttc agtatcagca acttcttttt atcgcgcaga
     9121 aagaaaacaa aagtaagtca gaaatcataa aggaactcat taccagggca taccgggcga
     9181 tacaataacc accacgttca ccatttcatc ccgaccatac atcatatgca cgggatgata
     9241 ctgactattt tttatcgtgc cgactgacta tgccaggtat acataacgaa tattcttttg
     9301 ttacatcgct tacatattcg cgtataagat cccttttctt attattatca cgtccacttt
     9361 ctattctgaa gaataattct cttaaactaa acctggacat tatatccgaa tgcaacaatg
     9421 taaa
//
LOCUS       DAZUCC010000191         9371 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZUCC010000191 DAZUCC010000000
VERSION     DAZUCC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.192"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            465..710
                     /gene="yahM"
                     /locus_tag="ACHY09_004037"
     CDS             465..710
                     /gene="yahM"
                     /locus_tag="ACHY09_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFS3886936.1"
                     /translation="MAVQLFKTLLNQIPLLSFLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(727..1398)
                     /locus_tag="ACHY09_004038"
     CDS             complement(727..1398)
                     /locus_tag="ACHY09_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFS3886937.1"
                     /translation="MMKLLHLFMVEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            1545..1820
                     /gene="yahO"
                     /locus_tag="ACHY09_004039"
     CDS             1545..1820
                     /gene="yahO"
                     /locus_tag="ACHY09_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFS3886938.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(1921..3507)
                     /gene="prpR"
                     /locus_tag="ACHY09_004040"
     CDS             complement(1921..3507)
                     /gene="prpR"
                     /locus_tag="ACHY09_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFS3886939.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGHEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFAAALRQGLQESETMLVHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            3746..4636
                     /gene="prpB"
                     /locus_tag="ACHY09_004041"
     CDS             3746..4636
                     /gene="prpB"
                     /locus_tag="ACHY09_004041"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFS3886940.1"
                     /translation="MYLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            4796..5965
                     /gene="prpC"
                     /locus_tag="ACHY09_004042"
     CDS             4796..5965
                     /gene="prpC"
                     /locus_tag="ACHY09_004042"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFS3886941.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            5999..7450
                     /gene="prpD"
                     /locus_tag="ACHY09_004043"
     CDS             5999..7450
                     /gene="prpD"
                     /locus_tag="ACHY09_004043"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFS3886942.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            7490..>9371
                     /gene="prpE"
                     /locus_tag="ACHY09_004044"
     CDS             7490..>9371
                     /gene="prpE"
                     /locus_tag="ACHY09_004044"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFS3886943.1"
                     /translation="MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVATRIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AME"
BASE COUNT         2269 a         2460 c         2539 g         2103 t
ORIGIN      
        1 tttctgatag tgtctggttt aatcagagct ttattatctg cgacgtttat ttttatttaa
       61 tagagccacc tgcaagttgt tattccacca gagagtgaat cgcaaagcag gtaatcattt
      121 catctgtata tctgtatgcc aactggcatt ccatctcttt tttcgccgat attttgttgt
      181 gatcggcgac acttcgctta aaaaagcatc agcagtggtt tcgcagctat gcggtgtata
      241 aaaaatgatc tcatgcagat gttttgtgaa tgtgtgggtt gacattcata tgaaaaaaat
      301 cataattcca tcatgtttgt gtggggtctt ttctgtatct tacgcatcgc actcaagcct
      361 gacagaaaat atgctgtaag gctcatatca aaaaccgcca ttagctcatc aggaagagca
      421 gacgacaacc aggattgttg tatggtacgg ggttcgaggc ctcgatggca gtccagcttt
      481 tcaaaactct tctgaatcag atccccttat tatcattcct gcaaagtgga acattgccat
      541 tgtttggcta ttcaggacgg gtcagaccaa tgaaaaaagc ccacacaggg gagagtgggc
      601 tgaaatggga agctaaagac tcaagtaaac ttatcggaaa taaggaccac gcattacggg
      661 ggctatcatc gccgatggtc gggattcggc aaatcaggtt gattacgtag agagtaaatt
      721 atctgctcac cgctgcgtca ccccttcgta aatcaggcgt agcgcgaata caccaataat
      781 tgcaccaata acccgactgg caacgcgttg catacgccca taagcacgac gcactgcggg
      841 caaagaaaac gcctgactaa gaaaaacgcg ccagataact gatgcgagca caatccccgc
      901 ccaggccatt aaacgtgccc atgttggtgt ttcggcattt aatgttactg agaaaatact
      961 aataaaaaat aaaacggttt gcgggttaga gagatcggta attaatccac ggcggaaaaa
     1021 gatataccag ggggcactaa tcggttgttg tagtgtgctc atttgcggtg ttgactggcg
     1081 gcgcatgctg caccacgcaa accataagag ataagcgccg ccgacgattc tgataagcga
     1141 aaaaatctcc tcacactgcg taattagtgt tgcaagacca aacaaaccca acccggaata
     1201 aaatgcatcg cccaacgcca cgcccagtcc ggtcagcacc cctgcgcgtc gaccggaagc
     1261 caggctggtt tgtactacca caaagagatt ggctcccgga ttaaaaaaag taatcacgaa
     1321 cagtcctacg gtcagataaa cggcgtgcaa aggatccata gtgatttcaa ccataaataa
     1381 gtgaagtaac ttcatcatat ttctaccaaa aataatcggg tgcgagagag atcacaaagt
     1441 gtcttatttc cggttactgg cgtttatgcc ctgactgaac taattattaa tcaacccaat
     1501 aatgtgggtg ggtgatagtg tgataacaac tctggagccg taatatgaaa ataatctcta
     1561 aaatgttagt cggtgcgtta gcgtttgccg ttaccaatgt ctatgccgca gaattaatga
     1621 ccaaagcgga atttgaaaag gttgcatcgc aatatgaaaa aataggtgat atttcaacca
     1681 gtaatgaaat gtcgaccgca gatgcgaaag aagatttgat caaaaaagcc gatgaaaaag
     1741 gggctgatgt gttggtactg acctctggtc aaactgacaa taagatccac ggcacggcaa
     1801 atatttataa gaagaagtaa ttctgaatcc tatgtaaaca tccccgatgc gtaagtttat
     1861 cggtgatcat catctattga aatttatgcc ggataaagcg ttcgcgctgc attcggcagt
     1921 tcagcttttc agccgccgcc agaacgtcgt ccggctgata cctaaataat tcgccgctgc
     1981 tgttttatcg ccattaaatt tctccagtgc ctgttgtgct gtcagcaagc ctggaatggg
     2041 agtcttcgcc gactcgcgtg ccagttccgg cagtagcagc tgcaaaaatt gcggcgttaa
     2101 atccggcgtc gattccacac ttaaaaacag cgccagtcgt tccatcatat tgcgcagttc
     2161 acgaatattg cccggccagt cgtagtgcac cagcatggtt tcgctttcct gtaatccctg
     2221 gcgtaatgcg gcagcaaacg gggcggagag cgccgccaga gacactttca aaaaactttc
     2281 cgccagcggc agaatatccg ccacccgctc gcgcagtggt ggcaattgca gacgcaaaat
     2341 actcagccga taaaacagat cacggcgaaa ctgcccttgc cgcatatctt cttccagatt
     2401 gcagtgagtg gcgctaatga ctcgcacatc caccggaaca ggctgatgcc cgccgacgcg
     2461 ggtgacctct ttttcttcca gcacccgcag cagccgagtc tgcaacggca gcggcatttc
     2521 gcctatctcg tcaagaaaca gcgtgccacc gtgggcgatt tcgaacagcc cggcgcgacc
     2581 tccgcgtcgc gagccggtaa acgccccttc ctcatggcca aacagctccg cttccagcag
     2641 cgattcggca atcgctccgc agttgacggc aacaaacgga tgcgactttt tgccctgtcg
     2701 cgcatcgtgg cgggcaaaat attcccgatg aatcgcctgg gccgccagct ctttgcccgt
     2761 ccccgtttcc ccctcaatca acaccgccgc actggagcgg gcatacagca aaatagtctg
     2821 ccgcacctgt tccatctgtg gtgattgacc gagcatatcg cccagcacgt aacgagtacg
     2881 cagggcgttg cgggtggcat cgtgagtgtt atggcgtaac gacatgcgcg tcatatccag
     2941 cgcatcgctg aatgcctggc gcacggtggc ggcggaatag ataaaaattc cggtcattcc
     3001 ggcttcttct gccagatcgg taatcagccc cgcgccgacc accgcttcgg tgccgttagc
     3061 tttcagctcg ttaatctgcc cgcgcgcgtc ttcttcggta atgtagctac gttgatcgag
     3121 gcgcaaatta aaggtttttt gaaacgccac cagtgccgga atagtttcct gataagtgac
     3181 aacgccgata gaagaggtga gctttccggc ttttgccaga gcctgtaaca catcgtagcc
     3241 gctcggttta atcaaaataa ccggcactga caggcgactt ttaagatatg cgccgttaga
     3301 gccagcagcg atgatggcgt cgcagcgttc gtttgccagt tttttgcgga tgtaggtcac
     3361 cgctttttca aagccaagct gaataggggt gatattcgcc aggtgatcaa actcgagact
     3421 gatatcgcga aacagctcga acagccgcgt tacagatacc gtccagatca ccggtttgtc
     3481 gtcattcagc cgtggtggat gtgccatagc gcaccgcaaa gttaagaaac cgaatattgg
     3541 gtttagtctt gtttcataat tgttgcaatg aaacgcggtg aaacattgcc tgaaacgtta
     3601 actgaaacgc atatttgcga attacttcat gactatattg ctaacaaatt gaaattaaac
     3661 atttaatttt attcagtcaa ttgtggcaca ccccttgctt tgtgtttatc aacgcaaata
     3721 acaagttgat aacaaaggat gggctatgta tctacactct ccaggtaaag cgtttcgcgc
     3781 tgcactgtct aaagaaaatc cattgcagat tgttggcacc atcaacgcta accatgcgct
     3841 gctggcgcag cgtgccggat atcaggcaat ttatctctcc ggcggtggcg tggcggcagg
     3901 gtcgctgggg ctgcccgatc tcggtatttc tactcttgat gacgtgctga cagatattcg
     3961 ccgtatcacc gacgtttgtt cgctgccgct gctggtggat gcggatatcg gttttggttc
     4021 ttctgctttt aacgtggcgc gcaccgtgaa atcgatgatt aaagccggtg cggcaggatt
     4081 gcatattgaa gatcaggttg gtgcgaaacg ctgcggtcat cgtccgaata aagcgatcgt
     4141 ctcgaaagaa gagatggtgg atcggatccg cgcggcggtg gatgcgaaaa ccgatcctga
     4201 ttttgtgatc atggcgcgca ccgatgcgct ggcggtagag gggctggatg cggcgattga
     4261 gcgtgcgcag gcctatgttg aagcgggtgc cgagatgttg ttcccggagg caattaccga
     4321 actcgccatg taccgccagt ttgccgatgc ggtgcaggtg ccgatcctcg ccaacatcac
     4381 cgaatttggc gcaacaccgc tgtttaccac cgacgaatta cgcagcgccc atgtcgcaat
     4441 ggcgctgtac ccactttcag cgttccgcgc catgaaccgc gccgctgagc atgtctacaa
     4501 cgtcctgcgt caggaaggga cgcagaaaag cgtcatcgac accatgcaga cccgcaacga
     4561 gctgtacgaa agcatcaact actaccagta cgaagagaag ctcgacgacc tgtttgcccg
     4621 tggtcaggtg aaataaaaac gcccgttggt tgtattcgac aaccgatgcc tgatgcgccg
     4681 ctgacgcgac ttatcaggcc tacgaggtga actgaactgt aggtcggata agacgcatag
     4741 cgtcgcatcc gacaacaatc tcgaccctac aaatgataac aatgacgagg ataatatgag
     4801 cgacacaacg atcctgcaaa acagtaccca tgtcattaaa ccgaaaaaat cggtggcgct
     4861 ttctggcgtt ccggcgggca atacggcgct ctgcaccgtg ggtaaaagcg gcaatgattt
     4921 gcattaccgc ggctacgaca ttcttgatct ggcggaacat tgcgaatttg aagaagtggc
     4981 gcatctgctg atccacggca aactgccgac ccgtgacgaa ctcgccgcct acaaaacgaa
     5041 actgaaagcc ctgcgcggtt taccggctaa cgtgcgtacc gtgctggaag ccttaccggc
     5101 ggcgtcacac ccgatggatg ttatgcgcac cggcgtttcc gcgctcggct gcacgctgcc
     5161 agaaaaagag gggcataccg tctctggcgc gcgggatatt gccgacaaac tgctggcgtc
     5221 acttagctcg attcttctct actggtatca ctacagccac aacggcgaac gcattcagcc
     5281 ggaaaccgat gacgactcca tcggcggtca cttcctgcat ctgctgcacg gcgaaaagcc
     5341 gtcgcaaagc tgggaaaagg cgatgcatat ttcgctggtg ctgtacgccg aacacgagtt
     5401 taacgcctcc acctttacca gccgggtgat tgcgggcacc ggctctgata tgtattccgc
     5461 gattattggc gcgattggtg cactgcgcgg gccgaagcac ggcggggcga atgaagtgtc
     5521 gctggagatc cagcaacgct acgaaacgcc ggacgaagcc gaagccgata tccgcaagcg
     5581 ggtggaaaac aaagaagtgg tcattggttt tggtcatccg gtttatacca tcgccgaccc
     5641 gcgtcatcag gtgatcaaac gtgtggcgaa gcagctctcg caggaaggcg gctcgctgaa
     5701 gatgtacaac atcgccgatc gcctggaaac ggtgatgtgg gagagcaaaa agatgttccc
     5761 caatctcgac tggttctccg ctgtttccta caacatgatg ggtgttccca ccgagatgtt
     5821 cacaccactg tttgttatcg cccgcgtcac tggctgggcg gcgcacatta tcgaacaacg
     5881 tcaggacaac aaaattatcc gtccttccgc caattatgtt ggaccggaag accgccagtt
     5941 tgtcgcgctg gataagcgcc agtaaacctc tacgaataac aataaggaaa cgtacccaat
     6001 gtcagctcaa atcaacaaca tccgcccgga atttgatcgt gaaatcgttg atatcgtcga
     6061 ttacgtgatg aactacgaaa tcagctccaa agtagcctac gacaccgcac attactgcct
     6121 gctcgacacg ctcggctgcg gtcttgaagc tctcgaatac ccagcctgta aaaaactgct
     6181 ggggccaatt gtccccggca ccgtcgtacc taacggcgtg cgcgtccccg gaactcagtt
     6241 ccagctcgac cccgtccagg cggcatttaa catcggcgcg atgatccgtt ggctcgattt
     6301 caacgatacc tggctggcgg cggagtgggg ccatccttcc gacaacctcg gcggcattct
     6361 ggcaacggca gactggcttt cgcgcaacgc ggtcgctagc ggcaaagcgc cgttgaccat
     6421 gaagcaggtg ctgaccgcaa tgatcaaagc ccatgaaatt cagggctgca tcgcgctgga
     6481 aaactccttt aaccgcgtcg gcctcgacca cgttctgtta gtgaaagtgg cttccaccgc
     6541 cgtggtcgcc gaaatgctcg gcctgacccg cgaggaaatt ctcaacgccg tttcgctggc
     6601 gtgggtggac ggtcagtcgc tgcgcaccta tcgccatgcg ccgaacaccg gcacgcgtaa
     6661 atcctgggcg gcgggcgatg ccacttcccg cgcggtacgt ctggcactga tggcgaaaac
     6721 gggcgaaatg ggttatccgt cagccctgac tgcgccagta tggggtttct acgacgtctc
     6781 ctttaaaggt gagtcattcc gcttccagcg tccgtacggt tcctacgtca tggaaaatgt
     6841 gctgttcaaa atctccttcc cggcggagtt ccactcccag acggcagttg aagcggcgat
     6901 gacgctctat gaacagatgc aggcagcagg caaaacggcg gcagatatcg aaaaagtgac
     6961 cattcgcacc cacgaagcct gtattcgcat catcgacaaa aaagggccgc tcaataaccc
     7021 ggcagaccgc gaccactgca ttcagtacat ggtggcgatc ccgctgctgt tcggacgctt
     7081 aacggcggca gattacgagg acaacgttgc gcaagataaa cgcatcgacg ccctgcgcga
     7141 gaagatcaat tgctttgaag atccggcgtt taccgctgac taccacgacc cggaaaaacg
     7201 cgccatcgcc aatgccataa cccttgagtt caccgacggc acacgatttg aagaagtggt
     7261 ggtggagtac ccaattggtc atgctcgccg ccgtcaggat ggcattccga agctggtcga
     7321 taaattcaaa atcaatctcg cgcgccagtt cccgactcgc cagcagcagc gcattctgga
     7381 ggtttctctc gacagaactc gcctggaaca gatgccggtc aatgagtatc tcgacctgta
     7441 cgtcatttaa gtaaacggcg gtaaggcgta agttcaacag gagagcatta tgtcttttag
     7501 cgaattttat cagcgttcga ttaacgaacc ggagaagttc tgggccgagc aggcccggcg
     7561 tattgactgg cagacgccct ttacgcaaac gctcgaccac agcaacccgc cgtttgcccg
     7621 ttggttttgt gaaggccgaa ccaacttgtg tcacaacgct atcgatcgct ggctggagaa
     7681 acagccagag gcgctggcat tgattgccgt ctcttcggaa acagaggaag agcgtacctt
     7741 taccttccgc cagttacatg acgaagtgaa tgcggtggcg tcaatgctgc gctcactggg
     7801 cgtgcagcgt ggcgatcggg tgctggtgta tatgccgatg attgccgaag cgcatattac
     7861 cctgctggcc tgcgcgcgca ttggtgctat tcactcggtg gtgtttgggg gatttgcttc
     7921 gcacagcgtg gcaacgcgaa ttgatgacgc taaaccggtg ctgattgtct cggctgatgc
     7981 cggggcgcgc ggcggtaaaa tcattcccta taaaaaattg ctcgacgatg cgataagtca
     8041 ggcacagcat cagccgcgtc acgttttact ggtggatcgc gggctggcga aaatggcacg
     8101 cgtcagcggg cgggatgtcg atttcgcgtc gttgcgccat caacacatcg gcgcgcgggt
     8161 accggtggcg tggctggaat ccaacgaaac ctcctgcatt ctctacacct ccggcacgac
     8221 cggcaaacct aaaggcgtgc aacgtgacgt tggcggatat gcggtggcgc tggcgacctc
     8281 gatggacacc atttttggcg gcaaagcggg cggtgtgttc ttttgtgctt cggatatcgg
     8341 ctgggtggtg gggcattcgt atatcgtcta cgcgccgctg ctggcgggga tggcgactat
     8401 cgtttacgaa ggattaccga cctggccgga ctgcggcgtg tggtggaaaa tcgtcgagaa
     8461 atatcaggtt agccgcatgt tctcagcgcc gaccgccatt cgcgtgctga aaaaattccc
     8521 taccgctgaa attcgcaaac acgatctctc gtcgctggaa gtgctctatc tggcggggga
     8581 gccgctggac gagccaaccg ccagttgggt gagcaatacg ctggatgtgc cggtgatcga
     8641 caattactgg cagaccgaat ccggctggcc gattatggcg attgctcgcg gtctggacga
     8701 caggccgacg cgtctgggaa gccccggcgt gccgatgtat ggctataacg tgcagttgct
     8761 caatgaagtc accggcgaac cgtgcggcgt caacgagaag gggatgctgg tggtggaagg
     8821 gccgctgccg ccggggtgta ttcagaccat ctggggcgat gacgaccgct ttgtgaagac
     8881 gtactggtcg ctgttttccc gtccggtgta tgccaccttt gactggggca tccgcgatgc
     8941 tgacggttat cactttattc tcgggcgcac tgacgatgtg attaacgttg ccgggcatcg
     9001 gctggggacg cgcgagattg aagagagtat ctccagtcat ccgggcgttg ccgaagtggc
     9061 ggtggttggg gtgaaagatg cgctgaaagg gcaggtggcg gtggcgtttg tcattccgaa
     9121 agagagcgac agtctggaag accgcgaggt ggcgcactcg caagagaagg cgattatggc
     9181 gctggtggac agccagattg gcaactttgg ccgcccggcg cacgtctggt ttgtctcgca
     9241 attgccgaaa acgcgatccg gaaaaatgct gcgccgcacg atccaggcga tttgcgaagg
     9301 acgcgatcct ggggatctga cgaccattga tgatccggcg tcgttggatc agatccgcca
     9361 ggcgatggaa g
//
LOCUS       DAZUCC010000192         9358 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZUCC010000192 DAZUCC010000000
VERSION     DAZUCC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.193"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..1157
                     /locus_tag="ACHY09_004045"
     CDS             33..1157
                     /locus_tag="ACHY09_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFS3886944.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     SPGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            1138..3441
                     /gene="trbC"
                     /locus_tag="ACHY09_004046"
     CDS             1138..3441
                     /gene="trbC"
                     /locus_tag="ACHY09_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFS3886945.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINLMNGGKSRSEVIQSGDKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            3908..4138
                     /locus_tag="ACHY09_004047"
     CDS             3908..4138
                     /locus_tag="ACHY09_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886946.1"
                     /translation="MKTNMGSTKNGARVPHVRNRFTSMDIGMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYF"
     gene            complement(4236..4667)
                     /locus_tag="ACHY09_004048"
     CDS             complement(4236..4667)
                     /locus_tag="ACHY09_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFS3886947.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(4735..7446)
                     /locus_tag="ACHY09_004049"
     CDS             complement(4735..7446)
                     /locus_tag="ACHY09_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS3886948.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDMQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPVSGHINRLSWSQERLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYEP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(7458..7790)
                     /gene="mobA"
                     /locus_tag="ACHY09_004050"
     CDS             complement(7458..7790)
                     /gene="mobA"
                     /locus_tag="ACHY09_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFS3886949.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            8023..8358
                     /locus_tag="ACHY09_004051"
     CDS             8023..8358
                     /locus_tag="ACHY09_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFS3886950.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKRAPKKTDSGR
                     NDGIDMNNN"
     gene            complement(8443..9291)
                     /locus_tag="ACHY09_004052"
     CDS             complement(8443..9291)
                     /locus_tag="ACHY09_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS3886951.1"
                     /translation="MNQTLPTADLNTDGTTDVIPSVAIDRIIAQRNEGISLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEHGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
BASE COUNT         2375 a         2600 c         2140 g         2243 t
ORIGIN      
        1 aaacagcatc ccgccgacaa ggaaaaagaa caatgaccat ccactacact gcaacactga
       61 ataataaaca cctgtccctg accgccctgt accctgacgg taatcgggag gtgattgcca
      121 tcctgccagt cacagaactg ggcgcactga ttaaccgtaa taacaggctg atgcacctgt
      181 ccttcagttc cccttttcgc gaaataaccg aaacggtgct tgttgatacc gttgatgcag
      241 aacgtcccgg acatcacatc ggcagaatgc tctcagcgat tgatgcctgc ctggatgccg
      301 aatatgccgg aaaaggaaaa catccctccc ggaaaaatgg cttctggcgg acactgatgt
      361 gtggctttat cgccggaatt gctctgccgg cgacagttgc aggttaccac tggatgaagg
      421 cagagccccc ttccacgctg gcttacccta catctcagga atggagagct gaaccagtaa
      481 ccgacacaga gacaccacgc ccacatacgg aacatcatgt taacagcatc ccggcccgcc
      541 cggctcccac acagcaggaa ccttcttctg cccccgcccc gacggcagtg acggaagaac
      601 aacacgttca ggccgctgag gcccgccgta atctggccac agtacttaaa cgcaacgcag
      661 accgggggat gttcaccatt aacctgtccc ctggtcatga acgcacgctg tacgccttcc
      721 ttgaccccgc atgcatcaac tgccgtctgc tggaacccgc gcttaagcaa ctggcccggg
      781 aattcagcgt ggtgatttat ccggtctccg tgattggcgg ggagtccagt acaaagcgga
      841 ctgctccgct gctgtgtgaa ccggactcac aaaaacgggc gaccggatgg catcagctgt
      901 attccgctga cagtggtatg gcccccaccg gcgatgcacc ggccgcacag gatggacagt
      961 gtctgaccgc cgcacgtgct gccatcgatg tcaataacca ggctttccgc cagttcggtt
     1021 ttggcggaac cccctgggtg ttatccgata ccggctggca cctgccgtcc ggcatcctga
     1081 aagacgccac ttccgttcgc ctcttcctga aagcaacaga tagcgaggag tatcacaatg
     1141 agccagcacc acgttaatcc tgacctcata caccgcacgg cctggggaaa cccgttgtgg
     1201 aatgcactgc ataacctgaa catcactggt ctgtgtctgg ccggcagtat cattacggcg
     1261 ctgatatggc cactggccct tcctgtctgc ctgctgttca ccctggtcac ctccgtgata
     1321 ttcatgctgc aacgctggcg atgccccctg cgtatgccca tgacactcag ccttgatgac
     1381 ccctcccagg acagaaaggt caggagaagc ctcttcagct tctggcccac cctgtttcag
     1441 tatgaagcgg atgaaacatt ccccgcccgg gggatttttt atgtcggtta ccgccgtatc
     1501 aacgatatcg gtcgtgagct ctggctgagc atggatgacc tcacccggca tgtcatgttt
     1561 tttgccacca ccggtggcgg taaaacagaa accacctttg cctggctgct caacccgctc
     1621 tgctggggaa gaggctttac ctttgttgac ggcaaggcac agaacgatac cacgaggaca
     1681 atctggtatc tctcccgacg ttttggcagg gaggatgata tcgaagtcat caacctcatg
     1741 aacggcggaa aatcccgcag tgaagtcatt cagagtggcg ataaatcccg cccacagtca
     1801 aacacctgga acccgttcgc attcagcacc gaagccttca ccgccgaaac catgcagtcc
     1861 atgctgcccc agaacgttca gggcggtgaa tggcagtcac gggcgatagc catgaacaag
     1921 gcactggtct ttggcacaaa attctggtgc gtccgggaag gtaaaaccat gtccctgcag
     1981 atgttacggg aacatatgac gctggaaggg atggcaaaac tctattgccg tggccttgat
     2041 gaccagtggc cggaagaagc catcgcacca ctgcgaaact acctgcagga tgtcccggga
     2101 ttcgatatgt cactggtgag aacgccgtca gcctggactg aggagccccg taaacagcat
     2161 gcttatctct ccggtcagtt ctcggaaacg ttcacaacct ttgccgaaac cttcggggac
     2221 gtctttgcgg cagatgccgg cgacatcgat attcgcgaca gtattcacag cgaccgcatt
     2281 ctgattgtga tgatccctgc actggacaca tcggctcata ccacgtcggc actcggtcgt
     2341 atgttcgtca cccagcagag catgctcctc gcccgtgacc tgggctaccg gctggaaggc
     2401 acagatgcac agaccctgga agtcaaaaaa tacaaaggca gcttccccta tatctgtatc
     2461 cttgacgaag tcggtgccta ctacacagac cgcattgctg tggaggccac ccaggtacgt
     2521 tcactggagt tttccctgat aatgacggcg caggatcagg agcgaattga agggcagacc
     2581 tccgccacca acaccgccac actgatgcag aacgccggca ccaaattcgc cggacgcatt
     2641 gtcagtgacg acaaaacggc aagaacggtg aaaaatgccg ccggtgaaga ggcacgggcc
     2701 agaatgggca gcctgcagcg ccacgatggt gtgatgggtg agtcctgggt ggacggtaac
     2761 cagatcacca tccagatgga aagcaaaatc gacgtgcagg atttgatcag actgaatgct
     2821 ggtgagttct ttaccgtttt ccagggggat gtggtgccgt cagcctctgt gtatattccg
     2881 gacagcgaga aaagttgcga cagtgatccg gtggtcatca accgctatat ttccgttgaa
     2941 gccccccggc tggagcgtct ccgccgtctc gtccccagga cagtgcagcg caggctcccc
     3001 acaccggagc atgtcagcag catcatcggt gtactgacag caaaaccatc ccgtaaacgt
     3061 cgtaaaaacc gtacagagcc ttaccgtatc attgatacat tccagcgcca gttggcaaca
     3121 gcgcagacat cactggatct gcttccgcaa tatgatacag atatcgaatc ccgggcaaac
     3181 gaactgtgga aaaaggctgt gcacactatc aataacacaa ccagggaaga acgccgggta
     3241 tgttatatca cactgaatcg tccggaagag gaacactccg gtcctgaaga tataccttca
     3301 gtgaaggcga ttctgaatac tctgctcccc ctggagatgc tgttacctgt accggatcta
     3361 actgcatccc cgccccataa aaaaaatgtc gctcagacac catcaggtgg aaaacaagag
     3421 agtcgtaaaa aaagattctg ataacacctg tatggctgat tttaataaca acctttacgc
     3481 gattattaat ctcctccgct caccatagta agcggaggat tcaggatttc aatccatcac
     3541 cagaggtaca ataaatgtgt attagtagcc cggaaacaaa ctcatggtct gttatttatc
     3601 gtaaaaattc cggagaagat atcaatatca cttccctgac gtttaaaaat agtcttctgg
     3661 cagcccggac actgatggtg cccgaaaact atatgatatg catactcaga aacggggaga
     3721 gggtcagacg atgggacaga gaaatacttg caggctcaaa cagatggtac aagtgttctc
     3781 ccgataattt cgaaatactg ggtaagctcc ccataataaa taaagtcaca aaactgataa
     3841 aatcataatc tacggatttt aatcagttaa gctaaatcag gatgaacgct ttgattatga
     3901 attatacatg aaaacaaata tgggcagcac aaaaaatggt gccagggttc cccacgttcg
     3961 taatagattc acttctatgg atattggtat gaattcccag atcctgccag acatgctgat
     4021 taattttgcc cttattaaca tcacagacag aaagaacgag ggtaccaata ccattgatgg
     4081 taactggcag gccgatgaag gacgccgtta ccgggataat gtaagaatct atttttgagt
     4141 atgtcaacat aacctgaggc aaagtacctc aggttccagt gtagtaatca gccaacctga
     4201 cagtcttctg ttagccctac agatatgtcg tggagctaac cgcgcatgtc aaagccggat
     4261 ttttgaaaat ctctataaac ttccggattg ttgaaggccg ggaatttagc tttatgggct
     4321 gctgacttta tcgcttcgca ataggcttta tcgccattac tggttgatat ttttaacgcc
     4381 gtgccatccc gggagaattc catatgcaac ctgcattttt tccttttcca tttttgcgga
     4441 tcatcaagtt tagcattaat tgcatccctg attccccgtg cttgctcccc ccattcatcc
     4501 tgatcatccc aacgtcctga actgcaacta cccagggctg tagttttatg gcaatttgaa
     4561 ggatgtaatg gcgcacatcc cgccacaaag ctgactaaaa tagccagcat aacgattttc
     4621 tttaccccca tatattgctc ctcaatccat atagatctca gtaccatagc tctgtcagag
     4681 caaagaaaat cttaactaat acaagttaag atttttaata tccactcctc atccctacat
     4741 tccaggtgat ggtggcgtcc actcattatc ctgaactgga gcataagatg cgccatgccc
     4801 tccggatatg cttttctgtt gcctgatata ttcgtggtag tactgttcac tacgctgacg
     4861 atgtaaatcc acatccgggc ggctgattac aaagtgtcca tttccggtta ttccagctgc
     4921 attatgccag gccaccattt ccgcaaatac atcattaaac tgttgatatg agccttccgg
     4981 ttcaaaaccc attcctggct cacgatcaaa caatacagga gcaatcagat tcagcttatc
     5041 caccagttca tttctgtcct gatgatggta gaacaccaca cggtccccat aatccacaca
     5101 gactaactcg ccattcctcc gatcacgaaa acgcacactg ccatctttct gcagacgggc
     5161 ttcgagaacg ccagtgtctt cataaagtgg agttcctccc ggttcacaga gaatcacgca
     5221 gcggtcggca ttcgttgttc gtcgcttgtc tttgcgacga tcacgataat cccagccacg
     5281 taactgtgat aatgcagctc tgtctccctg taccgcctgt tgctccaccc actgccggta
     5341 ggacaaagga taccacttac cctccgcaat cagtgacaac cgctcttcct tcacactctc
     5401 cttcagcctg atcagcgcct gcatacgctg cacttctgca atatggtaat gcagcttacg
     5461 caattgtgga tcccggaact gcacacggat atacgccttg cgccgacggc aggcagcatg
     5521 aatttcccgt aaccgttctc cgtaacgtaa gtcgggttta cgccagtgct ccttccatgc
     5581 aagataacgc gcgcgcaaat cttcccgcgc agctgcacgg gcttcacggc gttcacgacg
     5641 cagtacagga tcgcgccgga agccgggctc cacttcatcg ctggcagcaa gttccggatt
     5701 gtagcgacat tcaggcttca cccgttcaaa tatatctgca cctgcaatct caaatggccc
     5761 tgcctgcggc tcagccctgg ataacgtcaa atccgggtgg atactgctgg ccttcactgg
     5821 tgtgagctca tgattaaacg catccacaat cacaagacca tgatgctgtt tctgcaacat
     5881 aagcccttta cgcgcaaaca tctcatggca cgcctgccag ctgaaattcc cgcccgcgat
     5941 catatcttca atcaacgact cacactgaac agtcgtgtac ccccacagac tttccttatc
     6001 agaaaacagt tcgcgcttta ttgctccctg tggacctgtc cccatccgca tacgatacca
     6061 ttcgtaatca ctgacagcct gagtaccaag attacgtccc cggaatcgtt ctccgggtat
     6121 aacctgagta aaaatatcct tcggtacagg ctgccatcca ccatccagat cctgcatata
     6181 atccagcgtc attaacggcc acacactgtc tgcacgtact ggtgtctgtt gtttatcaaa
     6241 tccatcatgc agtaccagat gcccgtgctg gatccccatc cataatccag cctttgccag
     6301 agttctgtgc acatccaggc aactttccag ctcaccattt ctcgccattt ccggtaagcg
     6361 ttcagcaaag tgacgacagg cgtactgttt cagactctcc gtttctgcca ctttttccgg
     6421 ccggatatcc acgttaatgg cttccggctc ataacgaccg ggtgtcccca cctgactgaa
     6481 aatatctgtc ggtacaggct gatattcccc cagtttccgc cccagttttt cagctgtcca
     6541 tgacggtccg aacgaactga gtgcaacacc ttcccgcgct ctgtcccagc catccttcac
     6601 caccagttcc ccttcctgca tcgtgatata cagaccatca ctggccagcc gtttatgcaa
     6661 agataaccag tcctgtgcag gctcttcacg cagcccggca atcgacatct gagcaatata
     6721 ttccctgaac gtctgctttt tcccgcgtcg ccaggcatta cggcgctcac gtaccagcgc
     6781 tgttttgcgc acaatacggt tgccgggtgc atgcacaaag cagccattat caggcgcgaa
     6841 tccgtgctta agttcaagtt cacgacatgc acggctgagc ctttcctgac tccaggaaag
     6901 acgattaata tgaccactaa caggatgaac acgattcact gcgacatgta catgcaggtt
     6961 atccgtgtcc gtatgaacgg cagccacgta ctgatgtttt gagagttcaa gggatttcaa
     7021 tgtgtgacgc acacagtcaa aaagctgctc aggacgcggg ctttcatgcg caggccagga
     7081 gagaatgtag tggaaaacgg ggtccgtatc attacgggaa aaaacagctt tgtccgcctt
     7141 gtactgcatc tcttcagcag ctgtttccag tgaattgcag ttatggaaac aggtaacgcc
     7201 atagaaattt acccactgac tgccgtcttt catcacgtca atcagggaga caaatttttc
     7261 gttacgcagg cgtgtagcat agtcgacaag gcgtctgaaa cggttgcggt gagggacgtc
     7321 actcttgatt tcgctgtctt ctgtgagctc attttcctgc atatcatccc gcacagatgt
     7381 gtacgcaatc aggtcttcaa acgaagacct gccgtcacgt cttttgtgcg gaataatggc
     7441 attcatcagc agttatctca gcgtcccatt aagtctcttc gcagagcatt gacatattca
     7501 gtaatggcaa caagaacctc ggcatactcc ttatcaccag tacgttttcc ttccacaaac
     7561 agatgcttct gaaggcggcc aagacgcata atctcatcaa tcatacggtc gtcagtacgg
     7621 cagttaacca ccttatccag tgaagagctt cgtataaatg ttgaaaccgt ctgtccactt
     7681 gactccgctt tttctctgac gatttcagcc tcatcctccg tgaatctcgc cgtgaatttt
     7741 acaattctct tcctgttctc actgcccgtt cgggttttct tctcactcat ggtctgctcc
     7801 ttaccggggt gtcggggcga agccctgacc aggaggcatt tgtctgattg cgcgtgcgcg
     7861 gtccgacaaa tgcacatcct gtcccgtttt tcaggacatt atagcctgtt atttttgtgc
     7921 atcatagtct tatgcagacg gcagaggcag ggcgcagccc tgccaggagt cagtttttga
     7981 tgataaaatc aggcttcaac atgaaacaga gagaaagtgc caatggcttc taaaaaattt
     8041 tactcagatg atgacatcca gctggcaaaa gctgctttat cggaattgcc agatctcacc
     8101 gctcaacgga aaacattgcg tgattttctt gatgccattc gtgatgacat tattgtcctg
     8161 gtaaggacaa agggttatac actggcagat gttcgtgaca cgctcaaaaa tgcagggtat
     8221 gaagtaggag agaaggcact tcgtgacatt attcgggaag cagaatcgaa aaaaccatcc
     8281 cgccgttctt cctctaaaag agcacctaaa aagacagatt caggcaggaa tgatggcatc
     8341 gatatgaaca acaattgatg tgacaatgtc acatcgtgtt ctgagattat gatgattaca
     8401 gaaaaaaagc cccggcataa ataccagggc ttttctgttt aatcagactc gtggcggcaa
     8461 tgcaccagga taatgccgtg caataatatc attcagtttt tcaacaaggt caggacgctt
     8521 aaagacaatg tgtacggtcc ctttctggta gccacgcacc gtaaaatatt ccccttcaaa
     8581 caactcaccg acattgtcgc cgttcccgag aaattcacca tactgcgccc cgatgctgtt
     8641 cctgaagtca ggaacgttgc gcccctccag cagataaaac ggacgtacca ggtcatccag
     8701 tgcctcccgt ccgctgcagt ccagcgtcac acgcccgtta ctccagcggt acagcaggtt
     8761 ttcaataatg attttctttc ccagacggca gggattattg gttctgtaat cccatgacaa
     8821 tttccggtag acatcaatca gtccatgttc aaatgtctgc atcttactgg cattcagatg
     8881 gcgaaaagtt gccagtacat tgtccagggt gatttcagga caggtgtcgc tcatcagctg
     8941 gctgttccac tcatcacgct ggcatgaact catgaatgtg tacatgccag tatccgtcat
     9001 caggcgatcc cagattttcc ggtcggcaag acgggtgata ttcttttttg cttcttccgg
     9061 tttatccatg catcgaacgg agtcagtcac gcagtcttca aacccgtaga gaaaaatgtc
     9121 acctgacgca tcaagcagaa tcctgcgtgc tgtcgccagg cattccatcg cctgcatgaa
     9181 cagtgaaata ccttcgttac gctgcgcgat gatgcggtcg atagccacag acggaataac
     9241 atctgtcgtg ccgtcagtat tcaggtcagc agtgggtaaa gtctgattca tcacattgtc
     9301 tcctcatcgt cacgactgcc gggtcagcca ggtcatttcc cggaggagcg aagctcct
//
LOCUS       DAZUCC010000193         9120 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZUCC010000193 DAZUCC010000000
VERSION     DAZUCC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.194"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            123..485
                     /locus_tag="ACHY09_004053"
     CDS             123..485
                     /locus_tag="ACHY09_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886952.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(588..1046)
                     /gene="ybbJ"
                     /locus_tag="ACHY09_004054"
     CDS             complement(588..1046)
                     /gene="ybbJ"
                     /locus_tag="ACHY09_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS3886953.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1043..1960)
                     /gene="qmcA"
                     /locus_tag="ACHY09_004055"
     CDS             complement(1043..1960)
                     /gene="qmcA"
                     /locus_tag="ACHY09_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS3886954.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2106..2783
                     /gene="fetA"
                     /locus_tag="ACHY09_004056"
     CDS             2106..2783
                     /gene="fetA"
                     /locus_tag="ACHY09_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFS3886955.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2770..3549
                     /gene="fetB"
                     /locus_tag="ACHY09_004057"
     CDS             2770..3549
                     /gene="fetB"
                     /locus_tag="ACHY09_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFS3886956.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3612..4466)
                     /gene="cnoX"
                     /locus_tag="ACHY09_004058"
     CDS             complement(3612..4466)
                     /gene="cnoX"
                     /locus_tag="ACHY09_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFS3886957.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4527..5336)
                     /gene="ybbO"
                     /locus_tag="ACHY09_004059"
     CDS             complement(4527..5336)
                     /gene="ybbO"
                     /locus_tag="ACHY09_004059"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFS3886958.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHCGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5326..5949)
                     /gene="tesA"
                     /locus_tag="ACHY09_004060"
     CDS             complement(5326..5949)
                     /gene="tesA"
                     /locus_tag="ACHY09_004060"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFS3886959.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5920..6606
                     /gene="ybbA"
                     /locus_tag="ACHY09_004061"
     CDS             5920..6606
                     /gene="ybbA"
                     /locus_tag="ACHY09_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFS3886960.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6603..9017
                     /gene="ybbP"
                     /locus_tag="ACHY09_004062"
     CDS             6603..9017
                     /gene="ybbP"
                     /locus_tag="ACHY09_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFS3886961.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2074 a         2338 c         2508 g         2200 t
ORIGIN      
        1 ttgataagcg aaataaattc tgtttctgga ctaaacgtga agcggttcct gatgtgtgct
       61 aaaatgcgtt tctttgataa atctcaattt ttaagaggtt tacgccaggc aggaaaattt
      121 aaatgaatga atttgagaag atttttaatg aaatgaatct ggaccgggca ttgttaccga
      181 tcctttttcg ttcgaaccgg tcgacagtgt ggaagtattt aagcggcgat agtacggctc
      241 ctgcctctgc catgtcgctg atcatgctgt tgcagttgat ccagaaacga aaccccgatc
      301 tgttagccga atggttaacg ctttctgatt tcaccattcc gccagaagtt tacctggacc
      361 aacctgatta ctggaaaggg tgggtgtata cccaacacaa ggtcaataaa aacgtcctgg
      421 aatatctaaa aaaacactat ccggatgaag accaaaaaag tatgggtaaa ggccgggaag
      481 agtaattttg cctctactcg gctatatgcc ggatgcggcg caagcgcctt atctggccta
      541 cgattcgggc atattttgta ggcatgataa gacgcgccag cgtcgcatca ggcgatgacc
      601 gcacggataa tcagcgttat cccttctatc gcaatcacct caacatgcgt acctgcactg
      661 agatcctcgc tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg accacgcccg
      721 ttgaccagcg gagattccag aacaaaacgt cggccaatca actgctgccc gcgctggttt
      781 aaatgactgt cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt ccaccacagc
      841 caggcggcga gcagcgtcag gacggcaaac attacccctt gccactccca acccagcggc
      901 agcaaccaga ccaccagacc ggtaatcacc gctgccacgc cgctccacaa cagataacca
      961 ttgccgccca acatctcggc tgccagcagc aaaccgccga gacttagcca gaaaatatgt
     1021 ggatgaacga ccattaactc catcatggct gagtccgttt gttggcgctg tctttcacca
     1081 gctcagcaat cccggcaatc gaccccatca ggctgctggc ctctaatggc atcatcacca
     1141 ctttgctgtt actggacgaa ccgatctgct gtaacgcttc ggtgtatttc tgtgcgacga
     1201 agtagttcac cgcctgaata tcaccggagg caatggcttc agacaccatt ttagtggcgc
     1261 gggcttctgc ttcagcggaa cgttcacgcg cttcagcctg taaaaacgcc gactgacgtt
     1321 cgccttccgc tttcaggatt tgcgactgtt tttcaccttc ggctttgagg atttccgcct
     1381 gacggatccc ttccgcttca aggatgtaag cgcgtttggt acgttccgct ttcatctgcg
     1441 cgttcattga agagataagc tctgccggtg ggcgcacgtc gcgaatttca atacgggtga
     1501 ctttaatccc ccacgggttg gtggcctcat cgacaatacg cagcaggcgt gagttgatgc
     1561 tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc cagcacggta cggatgttag
     1621 tcatggtcag gttgatgatc gccagctcca gattgctgac ttcataagcc gcgcgcggcg
     1681 cgtcaatcac ctgaataaag cacacggcgt cgatggtaac gttggcgtta tctttcgaga
     1741 taacttcctg ggaagggata tcgagcactt gctccatcat attgatcttg cgaccaatgc
     1801 gatccataaa cggcaccacc agactgagcc ccggctgtaa cgttttggta tagcgaccaa
     1861 aacgttctac tgtccactga tagccctgcg gcacgatttt gacgcccgcg ccgacaatga
     1921 ccagcgcgac aaaaatgaga atcgggataa agataagcat cggaaaaacc tcctgttgta
     1981 ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat tatacctgat tactgaaaga
     2041 gagttccccc ttattcctgc gaaggataaa ctgtttttag taaaaatcag aaaaagggaa
     2101 cagtgatgca ggaaaatagt cctttgcttc agctacaaaa cgtaggatat ctggcgggtg
     2161 atgcgaagat tcttaataac atcaattttt cgctgcgtgc tggcgaattt aagttaatta
     2221 ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat agttgcttca ttgatcagcc
     2281 caaccagcgg aacgttactg tttgaaggtg aggatgtcag cacactaaag ccagaaatct
     2341 accgccagca agtctcttat tgcgcccaga cgccgacgct gtttggcgac acggtatacg
     2401 ataatctgat ctttccctgg cagatccgta accagcagcc tgacccagcc atttttctcg
     2461 attttctcga acgcttcgcc ttaccggaca ccattttgac gaagaatatc gccgagctat
     2521 ctggtggtga aaaacaacgc atctcattga ttcgtaacct gcaatttatg ccgaaggttt
     2581 tattgctgga tgaaataacc agtgcgctgg acgaaagtaa taaacataac gttaatgaga
     2641 tgatccaccg ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca cacgataaag
     2701 acgaaattaa tcatgcggat aaagtgatta cactgcaacc gcatgccgga gaaatgcagg
     2761 aagcacgcta tgaactcgca taatattact aacgaatcat tagcactggc attgatgctg
     2821 gtggtggtgg caatcttaat tagccataaa gaaaaactgg cgctggagaa agatattctc
     2881 tggagcgtcg ggcgcgcgat aatccagctg attattgtcg gctatgtgct gaagtatatt
     2941 ttcagcgtgg atgatgccag cctgacatta ttgatggtgt tatttatctg ctttaatgcg
     3001 gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag cttttatctc atcgtttatt
     3061 gccattacgg tcggggcggg aattaccctg gcggtgctga ttctctcagg gtcgattgaa
     3121 tttatcccga tgcaggtgat ccctatcgcc gggatgatag ccggcaacgc catggtagcg
     3181 gtggggttgt gttacaacaa tttagggcaa cgggtcatta gcgaacagca acagattcag
     3241 gagaaactga gtcttggcgc gacgccgaaa caggcttcag cgatattgat tcgcgacagt
     3301 attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa cggtaggctt agtgagttta
     3361 ccaggaatga tgtccgggct gatatttgcc gggattgatc cggtgaaggc gattaaatat
     3421 cagattatgg tgacctttat gctgctctca accgccagct tgtcgaccat tattgcctgc
     3481 tatttaacct atcgtaagtt ttacaattcg cgccaccaat tggtggtgac gcaattgaag
     3541 aagaaatgat gcgatgccgg acgcgcagca tcgcatccgg cgttgtggtt catgtgccga
     3601 tcaacggaaa ctcaatacaa caacgcgtac agctggcggc gatacttcga cgccagtgca
     3661 tcacccgtac ccagcgcagc gaggatctcc tggaatgttt tacgtgtctg accatctgcg
     3721 gcggtgagat ctttacgcag atgcccaaac agtaactcca gcgcctcttc attgcgccca
     3781 acctggtgca gttgcagtgc cagttgtgtc gccagtgcgg catcttctgg attctccgcc
     3841 acctgctgtt gcaactgttg aatttccggc gtatcagccg cctgcttcag cagttcgatt
     3901 tgcgccacca gcccctgata gcgggtgtcc tgatcctgca acggaatggt tttcagcacg
     3961 gcttctgcat cttcagaacg gttcagcgca atcagcgttt ctgccagcag caggccgatc
     4021 tccccgttct gattcgacaa ctgccaggcg tctttcagca atggcaaggc gtcggtgtaa
     4081 ttgccttcct gcatcagctg catcgcctgc tgcgctttca gctcttcttc gcgcggcagc
     4141 actttatcca gcagggcgcg gatcgcctct tccggttgcg gcccctggaa gccatctacc
     4201 ggttgcccgt tctggaacag atacacggtc ggaatcgcgc gcagaccaaa ctgggcggca
     4261 atcatctgct ccgcgtcgca gtccagcttc gccagaataa attgcccgtt gtactgcgcc
     4321 gcgaggcttt ccaggatcgg ggttaactgc aaacagtgct ggctacgttc agaccaaaaa
     4381 tagaacagca ccggagtggt catcgactgt tcaagaacct gttgcaggtt agattcgtta
     4441 atgttgacaa tattttctac ggacatggag tcgctctctg ttgtcgatat tttctttgac
     4501 atgggggctt aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat cacgcgcccc
     4561 ggcagcaggc gcttaagcac cattaccgcc caggtcacca gcgttaccgg atagcgcatc
     4621 ttcggcttct cgctaataaa agcatggcgt actttgtcca ccaccgcttc cggtcccaac
     4681 gtaaagcggg cggcgatgcc gggattttcg actggtttat cactttgcgt ctggttgacg
     4741 ttgtcggtga agcgagtacg aatgggaccg ggttcgatca ggctgacttt aattccacag
     4801 tggcgtagct ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt actggccgcg
     4861 taagcaccac gacccggcgt ggagattaat cccatcaccg atgaggtcat cacaatacgc
     4921 ccttcaccgt gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg tgcgccgaaa
     4981 aagttggcgg aaaactgctg ttccatctgc gcacggctga tggtggaaag ggggccatac
     5041 atgccgaatc cggcattgtt aaagatccca tacagacaat tatcggtcag ggcgatcacc
     5101 tcgtcggctg cgcgatcaac actttctggt gaatccagat cgatcaacac gccggtaaat
     5161 cccatgctgt tcatgcgctc aacatcatcc ggtttccggc aacctgccag cacatgaaaa
     5221 ccctggcgtt ttaattcgag cgcgctttcc aggccaattc cactggaaca tccggtaatt
     5281 aagaccgatt tttgcataac tttacctgtc aggatctccg ttgctttatg agtcatgatt
     5341 tactaaaggc tgcaactgct tcgccatcca gtcggcaata aacggctggg cgtcgcggtt
     5401 gggatgaata ccgtcatcct gcatccattg tggtttgagg tagacctctt ccataaaaaa
     5461 gggcagcagc ggaacatcaa actctttggc gagtttgggg taaatggcgc taaaggcttc
     5521 attataacgg cgaccatagt ttgcaggcag acgtatttgc attaacaatg gttcagcgtt
     5581 ggcggctttg acatcctgca atatctggcg cagcgtttgc tcggtttgtt gtggctgaaa
     5641 accacgcaaa ccgtcattgc cgcccagttc taccagcacc cagcgcggct gatgctgttt
     5701 cagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga tgctggcatt
     5761 gactaccgac gttttactct gccacttatc gttcaacaag gcaggccagg ccgcgctggc
     5821 agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt ccgctgcggc
     5881 ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga aaacattgtt
     5941 gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc catcctcacc
     6001 ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga gtcgggatcg
     6061 ggtaagtcaa ctttgctggc gatcctcgcc gggctggatg acggcagcag tggcgaagtg
     6121 agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa gttgcgcgcg
     6181 aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa cgcgctggaa
     6241 aacgtcgagc ttccggcgct gctgcgcggt gagagtagcg cggaaagtcg taacggggcg
     6301 aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc ggcacagctt
     6361 tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaacggtcg acctgatgtg
     6421 ctgtttgccg acgaacccac cggcaatctt gaccgacaga cgggcgataa aattgccgac
     6481 ctgctatttt ccctcaaccg tgagcatggc accacgttga ttatggtgac ccacgacctg
     6541 caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcaatt gcaggaggaa
     6601 gcatgattgc acgttggttc tggcgcgaat ggcgctcgcc gtcgctatta attgtctggc
     6661 tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc gatcgcatgg
     6721 agaagggctt aagccagcaa agccgggagt ttatggcggg cgatcgggcg ttgcgcagtt
     6781 cacgcgaagt gccacaagcg tggctggagg aagcgcaaaa gcgcggcctg aaagtcggca
     6841 agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg gcgaacgtca
     6901 aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc cctggcctga
     6961 aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt aacctgaaaa
     7021 caggcgacac catcgacgtg ggtgatgcca ccttgcggat tgccggagaa gtgattcagg
     7081 aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg aatctggcgg
     7141 atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgc tataaattcg
     7201 gcggcagcga gaaccagctc gacggctatg agaaatggtt gttaccccag cttaaacctg
     7261 aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg atagaacgtt
     7321 cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcggtg gcggcggttg
     7381 cggtggcgat gaatcattac tgtcgcagtc gctacgactt ggtggcgatc ctcaagacgc
     7441 tgggggcagg gcgagcgcaa ctgcgtaagt taatcgtcgg tcagtggttg atggtgctgg
     7501 cgctttcagc cgttaccggt ggtgcgatag gcctgttgtt cgaaaacgtg ttgatggtgc
     7561 tgctcaagcc cgttttgcct gctgcactac cgccagccag cctctggccg tggttgtggg
     7621 cgctgggcac catgacggtc atctcgctgc tggtggggct acgaccgtac cgcttgttgc
     7681 tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc tggccgctga
     7741 agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga ttaatgggtg
     7801 gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg ctgtgcggtg
     7861 tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg ctacctctgc
     7921 gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag ctttcggcat
     7981 tttcgctctc ctttatgctg ctggcgctgt tgctggtatt gcgtggcgat ctgctcgacc
     8041 gctggcagca gcagctacct ccagaaagcc cgaactactt tttaattaac atcgccacag
     8101 aacaggtagc accgctaaaa gcgttcctcg cggaacatca gatagtcccg gaatcgtttt
     8161 atccggtggt gcgggcgcgg ctgacggcga ttaacgataa gccgacagaa ggcaatgaag
     8221 atgaggcgct caatcgcgaa ctcaatctta cctggcaaaa tacgcggccc gatcataatc
     8281 cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa gaggggctgg
     8341 cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat acccaggagt
     8401 tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg cctaatttct
     8461 attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt accagtttcc
     8521 gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttccct actattagcc
     8581 tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag gtaagtcggg
     8641 cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg ctggcacagg
     8701 tgcaggtggg aatgcgtcag cgtcatcagg agctggtggt gtggcgcaca ctcggtgcgg
     8761 ggaaaaaact gctgcgtacc acgttgtggt gtgagttcgc catgcttggg tttgtttctg
     8821 gcctggtagc cgcaattggt gcggaaacgg cgctggcagt gttgcaggcg aaagtgtttg
     8881 attttccgtg ggagccagac tggcgattgt ggattgtgct gccgtgcagc ggtgcactgc
     8941 tgctgtcgct tttcggcggc tggctgggtg cgcgactggt taagggtaag gcgctgttca
     9001 ggcagtttgc gggatgatga gagtaaataa ggggcgttct ggttgaatgg aacgccttgt
     9061 ttaaattaat tctacaatat gtttcattga tgatgttatt gaattggtga tttcctatct
//
LOCUS       DAZUCC010000194         9107 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZUCC010000194 DAZUCC010000000
VERSION     DAZUCC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.195"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..1244
                     /gene="ompN"
                     /locus_tag="ACHY09_004063"
     CDS             111..1244
                     /gene="ompN"
                     /locus_tag="ACHY09_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFS3886962.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1385..1819
                     /gene="uspF"
                     /locus_tag="ACHY09_004064"
     CDS             1385..1819
                     /gene="uspF"
                     /locus_tag="ACHY09_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFS3886963.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1995..2930
                     /gene="ttcA"
                     /locus_tag="ACHY09_004065"
     CDS             1995..2930
                     /gene="ttcA"
                     /locus_tag="ACHY09_004065"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFS3886964.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3059..4432)
                     /gene="dbpA"
                     /locus_tag="ACHY09_004066"
                     /pseudo
     CDS             complement(3059..4432)
                     /gene="dbpA"
                     /locus_tag="ACHY09_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
     gene            complement(4462..4635)
                     /gene="ynaL"
                     /locus_tag="ACHY09_004067"
     CDS             complement(4462..4635)
                     /gene="ynaL"
                     /locus_tag="ACHY09_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFS3886965.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4910..5893)
                     /gene="zntB"
                     /locus_tag="ACHY09_004068"
     CDS             complement(4910..5893)
                     /gene="zntB"
                     /locus_tag="ACHY09_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFS3886966.1"
                     /translation="MEAIKGADVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5907..5954)
                     /gene="ydaN"
                     /locus_tag="ACHY09_004069"
     CDS             complement(5907..5954)
                     /gene="ydaN"
                     /locus_tag="ACHY09_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFS3886967.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6148..7380
                     /gene="dgcM"
                     /locus_tag="ACHY09_004070"
     CDS             6148..7380
                     /gene="dgcM"
                     /locus_tag="ACHY09_004070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFS3886968.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7401..7964)
                     /gene="smrA"
                     /locus_tag="ACHY09_004071"
     CDS             complement(7401..7964)
                     /gene="smrA"
                     /locus_tag="ACHY09_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFS3886969.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLTQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8159..>9107)
                     /locus_tag="ACHY09_004072"
     CDS             complement(8159..>9107)
                     /locus_tag="ACHY09_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3886970.1"
                     /translation="LSLLKVADYPSIILLAMTLGCLEYTLEEGARWGWLDDNTILLTS
                     VLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGGVVIFSTVYLILVFLG
                     QVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMAGLGGFVFSMYLFTPI
                     THEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTRNLGGAIG
                     IALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNRSGSDQTSEILASTKL
                     LSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
BASE COUNT         2464 a         2282 c         2098 g         2263 t
ORIGIN      
        1 ttaaggcgta acaaatgata tttatcggct aactgaactt ctcctttagg atgctttcac
       61 tccccaaaat ggggataaaa ggcaaataaa ataactaaag gatttattca atgaaaagca
      121 aagtactggc acttttaatt cctgccctgc tcgccgcagg tgctgcacat gcagccgaag
      181 tttataataa agacggcaac aaattagatc tgtatggcaa agttgatggc ctgcattatt
      241 tttctgataa ttcagcgaaa gatggcgacc agagctatgc gcgtctgggt tttaaaggcg
      301 aaacccaaat taacgatcaa ctcactggct acggtcaatg ggaatacaat attcaggcaa
      361 acaacactga atcttcaaca aaccagtcat ggacccgtct ggcatttgcc ggactgaaat
      421 ttgcagatta cggttctttc gattacggac gtaattatgg cgtaatgtac gacatcgaag
      481 gctggaccga tatgctgcct gaatttggcg gtgactctta taccaatgca gacaacttta
      541 tgactggtcg tgccaacggc gtcgcgactt atcgtaatac tgatttcttc ggtctggtaa
      601 atggtctgaa cttcgcggtg cagtatcaag gtaacaacga aggagccagt aatggtcagg
      661 aaggcaccaa caacggacgt gatgttcgcc atgaaaacgg tgacggctgg ggtctttcca
      721 caacatatga tttaggcatg ggctttagcg ctggtgcggc atacacctct tctgaccgca
      781 ccaatgacca ggttaaccat actgcggcgg gtggtgataa agcagacgcg tggactgctg
      841 ggctaaaata cgatgctaac aatatttacc tggcaaccat gtattcagaa acgcgtaaca
      901 tgaccccgtt tggcgacagc gattatgcag ttgcgaacaa aacccagaac tttgaagtta
      961 ctgcacagta ccagtttgat tttggtctgc gtccggcagt ctctttcctg atgtctaaag
     1021 gccgtgacct gcacgctgcc ggtggtgcag acaacccggc aggtgttgat gataaagatc
     1081 tggttaaata cgccgatgtt ggcgcgactt actatttcaa caaaaatatg tccacctacg
     1141 ttgactataa aataaacctg ttggatgaag atgacagctt ctacgctgcc aacggcatct
     1201 ctaccgatga tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg
     1261 ttatggcggg ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc
     1321 cgtcattaca caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg
     1381 ttttatgaac agaacgattc ttgtccctat cgatatttcc gattcagaat taactcaacg
     1441 cgtgattagc cacgttgagg cagaggcaaa gattgatgat gcagaggttc atttcctgac
     1501 ggtaatacct tcactgccct actatgcctc tctgggttta gcgtattccg cagaattacc
     1561 ggcaatggat gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt
     1621 taaactgcca accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat
     1681 tctggaattg gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga
     1741 tatcaccact tatctgctcg gttctaacgc cgcagcggta gtgcgtcacg cagagtgctc
     1801 cgtgctggtt gtgcgctgac attaacgccc gcacattgct gcgggctttt tgattcattt
     1861 cgcaaatgtg ctgacatttt cccctctaat ccgtaccata cacgccacag tttttatatc
     1921 agatttctta tgctgggcgt tccggcatga cgcatactct tctgatgcca tataacgaat
     1981 tgagtcgctt ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga
     2041 acaaattaca aaaacgtctg cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga
     2101 ttgaagaagg cgatcgcatc atggtttgcc tctccggggg taaagacagc tataccatgc
     2161 tggaaattct gcgcaatttg cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta
     2221 acctcgatca aaagcaaccg ggcttcccgg aacacgttct gcccgagtat cttgaaacgc
     2281 tgggcgttga gtacaagatt gtggaagaga acacttacgg tatcgtgaaa gagaagattc
     2341 cggagggcaa aaccacttgc tcactgtgtt ctcgtcttcg tcgcggtatc ctttatcgca
     2401 ccgcaacgga actgggggcg acgaagatcg cgctgggtca ccatcgtgac gatatcctgc
     2461 aaacgttgtt cttaaatatg ttctacggcg gcaagatgaa aggtatgcct ccgaaactga
     2521 tgagcgatga tggcaaacat attgtgattc gtccgctggc ctactgccgc gagaaagata
     2581 ttcagcgctt tgccgatgca aaagcgttcc cgattattcc gtgcaacctg tgcggttcac
     2641 agcctaacct gcaacgtcag gtgattgctg acatgttgcg tgactgggat aaacgttatc
     2701 cagggcgtat cgagacgatg ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg
     2761 ataccaacct gttcgatttc aaaggtatta cccacggttc tgaagtggtt aacgggggtg
     2821 atctggcgtt tgatcgcgaa gagatccctc tacaaccggc gggttggcag cctgaagaag
     2881 atgaaaatca gttggatgag ttacggctga atgtggttga agtgaaataa cccggatagc
     2941 gcccgatgcg caagcttacc gggctactct tatggaggcc ggataagacg cggccagcgt
     3001 cgcatccggc aatcccgaag aagatgttta ctcttgcacc ctgcgattca acatttcatt
     3061 attttaataa ccgcacccgg cacgtttttc ctttaatctt cccgccctgt aactgtttcc
     3121 atgctttatg agcaacagcc tgacgcaccg cgacatagac atgcgccgga tgcacggcga
     3181 ttttgccaat atctgcgcca tcaagcccga tatctcctgt cagcgcacct aatacatcac
     3241 ccgggcgcat tttggctttt ttcccgccat cgatacataa cgttgccatt tctgcttcca
     3301 gcggcacaat ggaactatta actggcggcg tttgccagtt aagttttatc tgcaacatgt
     3361 cagaaatgat attggcccgc tgtgcttctt ccggggcaca gaaactgatc gccagaccgc
     3421 tatttcctgc acgagctgta cgaccgatgc gatgtacatg aacttcaggg tcccacgcca
     3481 gctcaaagtt caccaccagc tcaagcgatt ttatatccag accacgcgca gcaacatcag
     3541 tcgcgaccag tacacgggcg ctaccgttag caaaacgtac cagggtctga tcgcgatcgc
     3601 gttgctccag atcgccgtgt aacgacaatg cactttgccc tacttcattc agcgcgtcgc
     3661 agacagactg gcaatctttt ttggtattgc aaaacaccac gcaagaggat ggctgatgca
     3721 agcttaataa ccgttgcaac agaggaattt tgcctttgct ggatgtctca taaaattgtt
     3781 gttcaatagg tggcagagca tctgttgagt caatttcaat cgctaaagga tcgcgttgca
     3841 ctcttccgct gattgcggcg atggcttccg gtcaggttgc cgaaaacaga agcgtctgtc
     3901 gagatgctgg cgcaaaacgg atgacatcat caatggcatc gctaaatccc atatccagca
     3961 tgcggtcggc ctcatccatc accagcgtat tcaacgcatc cagtgatacc gtgccttttt
     4021 gcagatgatc cagcatacgg cccggcgttg ccacgataat atgcggcgca tgttgcaacg
     4081 aatcacgctg cataccgaac ggttgaccac cgcacaacgt caaaattttg gtatttggca
     4141 gaaaacgcgc cagccgacgc aattcacctg ccacctgatc cgccagttca cgcgtaggac
     4201 acagcactaa agcctgggtt tgaaatagcg acgcatcaat ttgctgtaac aagccgaggc
     4261 caaaagccgc cgttttgccg ctgccggttt tcgcctgcac gcgaacatct tttccggcaa
     4321 ggatcgtcgg aagcgcggcg gcctgcaccg gcgtcatggt taaataaccc aactcattaa
     4381 ggttcgtgag ttgggcggga ggcaaaacat tcagggtaga aaaagcggac acaatttatt
     4441 ctcgtggtca tcgacgcaaa gtcagcaggc gcgtatcctc gcagatctac gctcacgatg
     4501 cgacaattta atcggttctt cgtcgggtgg tgggtcaggc atgggttgcg ggcgagggat
     4561 cggatcgggc actggaacag gatcgccagg aatcggttca gggacaggaa tttgcaaata
     4621 aataagtgtc gtcatatttc cctccggtca ttgggtggac tcttaaaggg tagacgctga
     4681 caaataacag gcaaaaaaaa gccgactcat caaagtcggc gtcgtacgaa tcaattgtgc
     4741 tatgcagtaa ttcaaaaaag gaagtaagac aatatggagc gcaacgccca tcgcttgacg
     4801 ttgcattcac ctgcaagaga gatattgccc tgaatgggta gagagtttat tgacttcgct
     4861 caaactttgc ggcgtttttg tatacagaca gccgggaaaa ttgcttttgt tacaaccatt
     4921 tactacgatg caaccataaa gcaacaccac caataagaac aactaacaga atacaaaaaa
     4981 ttgaaaatcc gaattgccac ccgccgcctg ggatcccacc aaggttgacg ccaaataacc
     5041 ctgtcagaaa ggtactgggt aaaaagacca ttgccatcaa cgacattgta taggtacgac
     5101 gcgctaaatt ttcctgcatc acctgagcga tttcatcagc catcacgcca gtccgtgcta
     5161 tacaggcgtc gatttcgtca aggccgcgcc caaggcgatc ggcaatatcc tgcatacgac
     5221 ggcgttggtc atcgctcatc cacggcaaac gttcactggc aagacgagca taaacatcac
     5281 gttgcggtgc catatagcga cgcatcacaa ttaattgttt gcgcagcaga gccaggaatc
     5341 cacgcggtgg aatttgctga tcaaggagat tatcttcaag gtcgataatt ttatcgtgca
     5401 gctgctcgat aaattcgctg gaatgatcgg tcaacgcatc acacacatcc accagccatc
     5461 ccccgcaatc ggtcggaccg gtgccttctt ccagatcgct taccacatcg tccagcgcca
     5521 gcactttgcg ttgtcgggtc gaaacaatta accgcccgtc catatataca cgcatggcga
     5581 ccagttgatc ggggcgttca tcggtgctgc cgtttataca gcgcaatgta atcagcgtac
     5641 cttcaccgag acggctgact cggggccgtg tgctctcgcc cgccagcgca tcacgtacgt
     5701 tattgggaag cagcggtgtt gtcgccagcc attgggcgct atcatggtgt acataattaa
     5761 ggtggagcca gcaggggtgc gcttcatcaa tcacatctgt attttccagc ggtttaacgc
     5821 cgcctctacc atccagcatc caggcaaata ctgcatccgg gacattaacg tccgctccct
     5881 taatcgcttc cacagtgcct ccatcatcaa cgcattattt tgtagtctag ccttctggcc
     5941 ctgttacgca acatctcatc accccattac cctgaaatga ttaataaaat tctgtctaaa
     6001 ttgaataaaa aaagcaaaat gcttttccat atacaaaccg tgtgaagtgt taaatagcgt
     6061 ctatcattat cagaattatc tgatcatatg acgtggcttt tttgcgatcg gatagcaaca
     6121 aaaattgata aaaataacgg gatctcaatg attacgcaca acttcaatac cctggactta
     6181 ctcaccagtc ctgtctggat cgtttcgccc tttgaggaac agttaattta tgccaacagc
     6241 gcggcgcgac tgttgatgca agacctcacg tttagtcagc tacgaaccgg tccctattcc
     6301 gtctcctcac aaaaagaact gccgaaatac ctctccgatc tgcaaaacca acacgatatt
     6361 atcgaaatcc tcaccgttca gcgcaatgaa gaggaaacag cattgagctg tcggcttgtt
     6421 ttgcgaaagc tgacagaagc agaaccggtg attattttcg aaggtatcga agcaccggca
     6481 acgctgggtt taaaagccag tcgctcggca aattatcagc gcaaaaaaca aggtttttat
     6541 gcgcgctttt ttctgactaa ctctgcacca atgttgttga ttgacccctc acgagatggc
     6601 caaatcgtcg atgctaacct cgccgcgctc aatttctatg gctataacca tgaaacgatg
     6661 tgccagaaac atacctggga aataaatatg ctcgggcgac gcgtcatgcc tatcatgcat
     6721 gaaatttcgc atttacccgg tggccataag cctttgaatt ttgttcataa actggcggat
     6781 ggttcgactc gtcatgtgca gacctatgcc ggtccaattg aaatttatgg cgacaagctc
     6841 atgttatgta ttgtgcatga tattactgag caaaaacggc tggaggagca gctggaacat
     6901 gctgcccacc atgatgcgat gaccggatta ctgaatcggc gacagtttta tcacattacc
     6961 gagccaggcc aaatgcagca cctcgccatc gctcaggatt acagcttgtt gctcatcgac
     7021 accgatcgtt ttaaacacat taacgatctc tatgggcatt ctaaaggtga tgaggtgtta
     7081 tgcgcccttg cccgcactct cgaaagttgc gctcgcaaag gcgatttggt gtttcgttgg
     7141 ggaggcgaag agtttgtctt attgctacca agaaccccac tggataccgc gctttcgctg
     7201 gctgaaacta tccgcgtaag cgtggcaaaa gtgagtattt cgggcttacc acgctttact
     7261 gtcagcattg gtgtggcgca ccacgaagga aatgaaagca tcgatgaact gtttaaacgc
     7321 gttgatgatg ctttgtatcg agcgaaaaat gatggacgca accgcgtgct ggcggcataa
     7381 gccacggatg cgtctcgtga tcaacgactg cgcttagcgt ggcgctccca gttttcttgc
     7441 ttcgcctgcg ctgttttacg tagtgcgacg taacacgccc cgctgccgcc atgatgcggt
     7501 agcgcagtgc aatatgcctg aacatcatca aattcggtca gccagcgcgc cacatagctg
     7561 cggacaatat tggcatgcga tttatcatcc cgtcctttgc catgaataat cagcacgtta
     7621 cgcaaaccat ccgccagggc ttgttgaatg aaactgaaca ccattttgcg gcactcttcc
     7681 accggctggc gcaaaagatt caggctcgcc tgttgcggat atttaccact gcgcagctta
     7741 tccagcaccc catgttgcaa cccttcccgc cgaaactcca gcggctgagt tagcgggatg
     7801 atgtcgagaa atccggtggt gaggaaatta tcaagctgca gcgtgtcgat acgctgcggc
     7861 gcacgttggt tacgcgttgg atgccagtgg acatctgtag cacgtttcag cggctggaca
     7921 tcttccatgg cgtcaagaaa cagcgatttg tcgtcaaggt tcatgtaacc tcctatgcac
     7981 cttcacacgt cgtgattcag acgggttaat tgttcatact tactgcatcg ttcccttcac
     8041 agattgaaac aatactctac ttcatacctg tgagtatctg cgcaggcggg gcattttata
     8101 ctagcctgtt agcagtatcg aacagtttag cccgagttta attcagcaca ggctccgatc
     8161 aggatgattt attcatagca ggaaccaaca aaaatgcgat aaataacaga ccactgatta
     8221 gcagaaaggt atccgaaaat gccatagttt gcgcctccct cagcatcaac tgactcaata
     8281 atttggttga agccagtatt tccgatgtct ggtcacttcc actgcgatta aaaaacaaag
     8341 cacttctgga aatgaagtca ttcaccgtat ggggaacaga aaccattttt tcacccattc
     8401 tcgaaaaatg aaaattagtt cgattattga gaatagagcc acacagggca ataccaatcg
     8461 cgcccccaag attacgtgtt aaattaaata caccagaggc taacttcagc cgttcctttg
     8521 gaattccccc cagcgttaat gttacgatgg gggccatcgc aaactgctgc gaaatccctc
     8581 taattgcctg tggaaacagt aactcctgcc atccccattc gtgggttata ggggtaaaaa
     8641 gatacattga aaacacaaat ccacccagcc ccgccatcaa cagccatcgc agatttattt
     8701 ttttacttaa ccagaagtaa aagggaacag aaaataactg gaaaatacct gttgtgcaaa
     8761 cggcaaaacc tatctcttcg gcatttaatc cgcgaacctg ccccaaaaac acaagtatta
     8821 aatatacagt agagaatatc acaacccctc cagaaaaaga aaaaaaacat cccagcgtga
     8881 aatttttatc cttaaaagca tgaagatcca ttatcggatt ggatattgta agtgtacgcg
     8941 cagcaaataa aataaaactc accaatgcca gaactgatgt cagcaaaata gtattatcat
     9001 ccagccatcc ccagcgcgct ccttcttcaa gggtatattc cagacatccc agtgtcatgg
     9061 ccaacaggat aatactcgga taatcagcaa ctttcagtaa agagaga
//
LOCUS       DAZUCC010000195         9070 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZUCC010000195 DAZUCC010000000
VERSION     DAZUCC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.196"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..574
                     /gene="lpfB-O113"
                     /locus_tag="ACHY09_004073"
     CDS             <1..574
                     /gene="lpfB-O113"
                     /locus_tag="ACHY09_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFS3886971.1"
                     /translation="DKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLE
                     PAQEKVLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRP
                     ASIRLIPEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTL
                     PLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            599..3121
                     /gene="lpfC-O113"
                     /locus_tag="ACHY09_004074"
     CDS             599..3121
                     /gene="lpfC-O113"
                     /locus_tag="ACHY09_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFS3886972.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQSLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFVAIKKTLTTFGVK
                     VDALKSLNDVDETVCIDPGPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPSRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTTDGS
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQVQILHGFPYGFTLYGGMQA
                     AEKYGSAALGVGKELGALGAISFDVTHARAKFSHADTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNNPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTRVGYRVLFRVAGTKGKPAPFGAIATVQNTSSADSGIVGDQGELYLSG
                     LPEKGQVMLSWGENAATTCTFDYSLSIPESESGLIEQGVTCH"
     gene            3132..4202
                     /locus_tag="ACHY09_004075"
     CDS             3132..4202
                     /locus_tag="ACHY09_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3886973.1"
                     /translation="MNKYIIPWCFIVFIFSFSGATFAAPKGICTSDNGSFHSTLDFSG
                     YLITADENRVGKTFNKTVTNGDSYPAHCHCDTGNVGEFPYIYYTARINESLGYAGVRS
                     NVNYFNLNPNLDVGISIDLLGVGYINAPFEYHANRPTGGSYKCSRTDPLSISSGAKAI
                     IYFYIKKTFAGKLIIPETLVAKLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINS
                     LRPINFDFKEIPAADFSSVVGSTVTTHKITKTVTIECENLGILNTDDISTSFYATEPS
                     TDNSMVVTSNPNVGIKIYDKNNKEINVNGGELPTDMGKSTVFGEKAGSVTFSAAPASL
                     TGARPAPGTFTATATITIEIVR"
     gene            4450..5490
                     /gene="pstS"
                     /locus_tag="ACHY09_004076"
     CDS             4450..5490
                     /gene="pstS"
                     /locus_tag="ACHY09_004076"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS3886974.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            5577..6536
                     /gene="pstC"
                     /locus_tag="ACHY09_004077"
     CDS             5577..6536
                     /gene="pstC"
                     /locus_tag="ACHY09_004077"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFS3886975.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALTVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            6536..7426
                     /gene="pstA"
                     /locus_tag="ACHY09_004078"
     CDS             6536..7426
                     /gene="pstA"
                     /locus_tag="ACHY09_004078"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS3886976.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            7517..8290
                     /gene="pstB"
                     /locus_tag="ACHY09_004079"
     CDS             7517..8290
                     /gene="pstB"
                     /locus_tag="ACHY09_004079"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS3886977.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            8305..9030
                     /gene="phoU"
                     /locus_tag="ACHY09_004080"
     CDS             8305..9030
                     /gene="phoU"
                     /locus_tag="ACHY09_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS3886978.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
BASE COUNT         2300 a         2144 c         2318 g         2308 t
ORIGIN      
        1 agataaatct atttctgtat ttagcaagga agaaaaaatt ccttatctca tccaggcatg
       61 ggtagatccg tttaataaag aggataaaag caaagcaccg tttaccgtta ttcctccggt
      121 atcacgatta gaacccgcgc aagaaaaagt attacgcatt attcatacca aaggtgtgtc
      181 attgcctgat gatcgcgagt cagtcttctg gctgaatatt aaaaatattc cgccctctgc
      241 gagcaacaaa gcggcgaact cactggaaat cgcagttaaa acgcgtatca agctgttctg
      301 gcgaccggca agcattcgcc tgattcccga agatgctgca ccgaaggtga agtggcaccg
      361 ggaagggcga aacctgattg cagagaatcc caacccgatt catatcagtg tgatggatgt
      421 catcgttgat ggtcatgatg tgcccctcaa catgatccgt ccttttgaaa cgctcacgtt
      481 acctttacct gctaattctg cgggaggtca aatgacctgg cgatttatta atgattacgg
      541 cgcagtcagt gacccgataa aaatgacgct ataagcaagg gataaggata ggcgtattat
      601 gatgacgacc agaatagtgg ttggcctcac ggcagggacg tgtctgattt tctcgcaaag
      661 cctgatggcc gaggtcagtg tattcaatcc ggcgcttctg gaaatcgacc atcaatccgg
      721 agtcgatatt cgccagttta atcgggcaaa cctgatgccc cctggtgttt atagcgttga
      781 tatttttatc aacggtaaaa tgtttgaacg tcaggatgtg acctttgttc aggataatcc
      841 cgatgctgat ttacacgcct gctttgtcgc tattaaaaaa acgctaacca cctttggcgt
      901 aaaagttgat gcgctgaaat cgctcaatga tgtagatgaa acagtctgta ttgatcctgg
      961 gccacgcatt gaaggctcat catggcagtt cgacagcgat aaactgcaac tgaatatctc
     1021 cattccccaa atctatatgg atgcgatggc ttatgattac atcagcccct cgcgttggga
     1081 tgaggggatt aacgcgctca ccatcaacta cgatttttct ggttcacata ctctacgttc
     1141 ggattatggt tcacaagaga cagataccag ttatctcaat ctgcgcaatg gactgaatat
     1201 tgggccgtgg cgattacgca attacagcac cttaaatacg accgatggca gtgcagaata
     1261 caactctata agcacctgga tacaacgtga tatagctgcg ttaagaagcc agattatgat
     1321 aggtgatacg tggacggcga gcgatatctt cgacagtacg caaattcgtg gcgcacgctt
     1381 gtataccgat aacgatatgt tgcctgccag ccaaaacgga tttgcccccg tagtgcgtgg
     1441 gattgcaaag tctaacgcca ctgtcattat tcgccagaat ggctacgtga tttatcagtc
     1501 agccgttcca caaggtgctt ttgaaattac cgacctcaac accgccagta caggtggtga
     1561 tctggacgta accatcaaag aagaagacgg tagcgaacaa cgatttactc aaccttatgc
     1621 gtcgttagcc atcctcaaac gtgaaggtca aactgatgtt gatgtcagtg tgggagaatt
     1681 acgcgatgaa gatggcttta cccctgacgt tcttcaggtg cagatccttc atggttttcc
     1741 ctacggtttc accttgtatg gaggtatgca ggctgctgaa aagtatggtt ctgccgcttt
     1801 gggtgtcggt aaagagcttg gcgcactggg tgcaatttct ttcgatgtga cacatgcccg
     1861 tgcgaaattt agccatgctg atacggaaac cggtcagtct tatcgctttc tgtattcgaa
     1921 acgctttgac gatacggata ccagcttgcg cctggttggc tatcgttact ccaccgaagg
     1981 ttactatact ctcaatgaat gggcgtcgcg gcgtaataat cctgaagatt tctgggaaac
     2041 cggtaaccgc cgcagccgtg tggaaggaac gttaacgcaa tcactgggca gagactatgg
     2101 caacttatat ctgacattaa gtcgccagca gtactggcat accgatgatg ttgaacgatt
     2161 aatgcaattt ggctacagca gtagctggaa gcgtctctcg tggaacgtct cctggagtta
     2221 ttccaatact gcccggcagg ggacggggaa caaccatgcc agtgataaca ccagtgagca
     2281 gatctacatg ctctctttat ctgttccttt atcgggctgg tggggaaata gttacgccac
     2341 ctattctgtt tcacaaaacg ataattccgg tagctcacat caacttggac tcagcggtac
     2401 ggcgctggaa agaaataacc tttcctggaa tttaatgcag tcctataaca gccatgatga
     2461 tgaggttggc ggtaatatgt ccctgaccta tgatggcact tatggcacgg tgaacggcag
     2521 ctataactac agccaaaatt ctcagaggct gaattatggt atcagagggg gaattctggc
     2581 gcacagcgaa ggcgtaacgt taagccagga gttgggtgaa accatagctc ttgttaaagc
     2641 accaggggct gccgggttag aaatagacaa tatgcgcggt gctgcgacgg actggcgcgg
     2701 ctatacggtc aagacacagc taaaccctta tgatgaaaac cgggtagcaa tcagcgataa
     2761 ctatttctcg aagtcgaata tagaacttga taataccgtc gttacgatgg ttcccacccg
     2821 tggtgcagtg gttaaggccg aatttgttac tcgtgttggt tatcgtgtgc ttttcagggt
     2881 tgcgggtaca aaaggtaaac cagcaccttt tggcgctatt gctacagtac aaaatacaag
     2941 ctccgctgat tcaggaattg tcggtgacca gggggagctt tatctctcgg gccttcctga
     3001 aaaagggcag gtgatgctct cctggggaga aaatgccgcc actacatgca ccttcgatta
     3061 ttcactttca ataccagaaa gtgaaagcgg cttaattgaa caaggtgtga catgtcatta
     3121 aaaggatttt tatgaacaag tatattatac catggtgctt tattgttttt atcttctcct
     3181 ttagtggcgc aacatttgcg gccccaaaag gtatctgcac ttcagataat gggtcctttc
     3241 atagcacact tgatttttcc ggctatctga ttacggcaga cgagaacaga gtgggaaaga
     3301 cctttaataa aaccgtgaca aatggggact cttatcctgc tcactgccat tgtgatacag
     3361 ggaacgtggg agagtttcct tatatttatt atacagcgag aataaacgag tccttaggtt
     3421 atgcaggtgt tcgttctaat gtaaactact ttaatttgaa tcctaatctg gatgttggaa
     3481 tatcgataga ccttctcggt gtaggataca ttaatgcacc ttttgaatac catgctaaca
     3541 ggcctactgg aggttcatat aaatgtagtc gcacggaccc attaagtatt tccagtggtg
     3601 caaaagcaat aatttatttt tatattaaga agacttttgc tggaaagtta attattcctg
     3661 aaactctcgt ggcgaaattg tacggaacca taagccgtga cactccggtt gattactcac
     3721 aacctatggc ggatgtttat attcgtggcg atatcactgc accgcaaagc tgtgaaatta
     3781 acagtttaag acccattaat tttgatttta aagaaattcc tgcggcagat ttttcttcgg
     3841 tagttggaag taccgtgaca acgcataaaa ttaccaaaac cgtcaccatt gagtgtgaaa
     3901 atttaggaat actaaatact gatgatatca gtacctcttt ttatgctacc gaacccagta
     3961 ctgacaactc aatggtcgtg acatcaaacc cgaacgttgg gattaaaatt tatgataaaa
     4021 ataataagga aatcaatgtt aacggtggtg aactgccgac agatatgggt aaatcaaccg
     4081 tctttggtga aaaagccggt agcgtcactt tttctgctgc tcctgcaagc cttacggggg
     4141 ctcgccccgc gccaggaaca tttaccgcga ctgcaacgat aacaattgag attgtacgct
     4201 aaacgaactg acagcggtgt tcctcacacc gttgtcatct tacgcatttt tgtccgcgag
     4261 ttttcactct gcatgacaaa aagatgacat tacttttctt tttcttagtc aataaaatca
     4321 aaaagttaat taataattct gatttgtcat aaaactgtca taaaccttac atataactgt
     4381 cacctgtttg tcctattttg cttctcgtag ccaacaaaca atgctttatg aatcctccca
     4441 ggagacatta tgaaagttat gcgtaccacc gtcgcaactg ttgtcgccgc gaccttatcg
     4501 atgagtgctt tctctgtgtt tgcagaagca agcctgacag gtgcaggtgc aaccttccct
     4561 gcgccggtgt atgccaaatg ggctgacact taccagaaag aaaccggtaa taaagttaac
     4621 taccagggta tcggttcttc cggtggcgta aaacagatta tcgctaatac cgttgatttc
     4681 ggtgcctctg acgcgccgct gtctgacgaa aaactggctc aggaaggcct gttccagttc
     4741 ccgaccgtga ttggcggcgt ggtgctggcg gttaacattc cagggctgaa gtctggcgaa
     4801 ctggtgctgg atggtaaaac cctcggcgac atctacctgg gcaaaatcaa gaagtgggat
     4861 gatgaagcca tcgccaaact gaatccgggt ctgaaactgc cttcacagaa cattgctgta
     4921 gtacgccgcg cagatggctc cgggacttcc ttcgtcttca ccagctacct ggcgaaagtg
     4981 aacgaagagt ggaaaaacaa cgttggtact ggctctaccg taaaatggcc gatcggtctg
     5041 ggcggtaaag gtaacgacgg tatcgccgcg ttcgttcagc gtctgccggg tgcaattggt
     5101 tacgttgaat atgcttacgc gaagcagaac aacctggcgt acaccaaact gatctccgct
     5161 gatggcaaac cggtgagtcc gaccgaagaa aacttcgcta atgcagcaaa aggcgcagac
     5221 tggagcaaaa cattcgctca ggatctgacc aaccagaaag gcgaagatgc atggcctatt
     5281 acctctacca cgttcattct gatccataaa gatcagaaga aaccagaaca aggcaccgaa
     5341 gtgctgaagt tcttcgactg ggcatacaaa accggggcta aacaggcgaa cgacctggat
     5401 tatgccagcc tgccggatag cgtagttgaa caggttcgcg ctgcgtggaa gaccaatatt
     5461 aaagacagta gcggcaagcc gctgtactaa taaaactcca ggccgggtac ggtgttttac
     5521 gccgcatccg gcattacaaa atgactttgt aaacgcgttt aactgaagag taacttatgg
     5581 ctgcaaccaa gcctgctttt aacccaccgg gtaaaaaggg cgacataatt ttcagcgtgc
     5641 tggtaaaact ggcggcgctg actgtgctat tgatgttggg tggcattatt gtctctctga
     5701 tcatctcatc ctggccgagc attcagaaat ttggtctggc tttcctgtgg accaaagagt
     5761 gggatgcgcc gaacgatatc tacggggcgc tggtgccgat ctacggtacg ctggtgacct
     5821 cgtttatcgc gctgctgatc gccgtcccgg tgagttttgg tatcgccctg ttcctgaccg
     5881 agcttgcgcc tggctggctg aaacgcccgc tgggtatcgc cattgagctg ctggctgcca
     5941 ttccaagtat cgtttacggc atgtggggct tgtttatctt cgccccgctg ttcgccgttt
     6001 acttccagga gccggtcggc aatatcatgt cgaatatccc gattgtcggc gcgctgttct
     6061 ccggcccggc ctttggtatc ggtatcctcg cggcaggcgt gatcctcgcc atcatgatta
     6121 ttccgtacat tgcggcggta atgcgcgatg tattcgaaca aaccccggtg atgatgaaag
     6181 agtcggccta cggtattggc tgcaccacct gggaagttat ctggcgtatc gttcttccgt
     6241 tcaccaaaaa tggtgttatc ggcggcatca tgttggggct ggggcgcgcg ctcggtgaaa
     6301 ccatggcggt gacctttatc atcggtaaca cctaccaact cgacagcgcc tcgctgtata
     6361 tgccgggcaa cagtatcacc tctgcgctgg ccaacgaatt tgcggaagca gagtcgggtc
     6421 tgcatgttgc tgcactgatg gaactgggcc tgatcctgtt tgtgattacc ttcatcgtcc
     6481 tcgccgcatc gaagtttatg attatgcgcc tggctaagaa tgagggggca cgctaatggc
     6541 tatggttgaa atgcaaacca ctgcggcgct ggctgaatcc cgccgtaaaa tgcaggcgcg
     6601 tcgccgcctt aagaaccgta ttgcgctgac gctctcgatg gcgacgatgg ccttcggtct
     6661 gttctggctg atctggattt taatgtccac catcactcgc ggtatcgatg gtatgtcgct
     6721 ggcgctgttc accgaaatga cgccgccgcc aaatacggaa ggcggtggtc tggcgaacgc
     6781 cctggcgggt agcggcctgt taattttgtg ggccacggta ttcggtacgc cgctgggcat
     6841 tatggcgggg atttatctgg ctgaatatgg tcgtaaatcc tggctggcag aagtgattcg
     6901 cttcattaac gacattttgc tctccgcacc gtcgattgtg gttggtcttt ttgtttacac
     6961 catcgtggtg gcgcagatgg agcacttctc cggctgggcg ggcgtgattg cgctggcgct
     7021 gttgcaggtg ccgattgtta tccgcaccac cgagaacatg ctgaaactgg tgccggacag
     7081 cctgcgtgaa gcggcttatg cgctgggtac gccgaagtgg aagatgattg ccgcgatcac
     7141 gctgaaagcg tcggtgtccg ggatcatgac cggtatcctg ctggcgattg cccgtattgc
     7201 tggtgaaacc gcgccgctgc tgtttactgc gctctccaac cagttctgga gcacggacat
     7261 gatgcagccg atcgccaacc tgccggtgac gatctttaag tttgcgatga gcccgtttgc
     7321 ggaatggcag caactggcct gggccggggt gttgatcatt accctgtgcg tactgctgct
     7381 gaacattctg gcgcgcgttg tttttgcgaa gaataaacac ggttaacact ttgctggatg
     7441 cggcgtaaac gccttatccg gcctacggta agcctgattc gcgaagtgca tcaggcacga
     7501 tgaggaaaag attgcaatga gtatggttga aactgccccg agtaaaattc aggttcgtaa
     7561 tttgaacttc tactacggca aattccatgc cctgaaaaac atcaacctgg atatcgccaa
     7621 aaaccaggtt acggcgttta tcgggccgtc cggctgcggt aaatcaacgc tgctgcgtac
     7681 cttcaacaaa atgtttgaac tgtacccgga acagcgtgcg gaaggtgaaa ttctgctcga
     7741 tggcgacaac atcctgacca actctcagga tatcgccctg ctgcgtgcga aagtgggcat
     7801 ggtgttccag aaaccgacgc cgttcccgat gtccatctac gacaacatcg cttttggcgt
     7861 gcgtctgttt gagaagctct cccgtgctga catggacgag cgcgtgcagt gggcattgac
     7921 caaagccgca ttgtggaacg aaaccaaaga taaattgcac cagagcggtt actctctctc
     7981 tggtggtcag caacagcgtt tgtgtattgc gcgtggtatc gccattcgcc cggaagtgct
     8041 gctgctcgac gaaccatgtt cggcgctcga ccccatctcc accgggcgta ttgaagagct
     8101 gattaccgaa ctgaagcagg attacaccgt ggtgatcgtc acccacaaca tgcagcaggc
     8161 tgcgcgttgt tccgaccaca cggcgtttat gtacctgggc gaattgattg agttcagcaa
     8221 cacggacgat ctgttcacca agccagcgaa gaaacaaaca gaagactaca tcaccggtcg
     8281 ttacggttga ttcaggagtg cgttatggac agtctcaatc ttaataaaca tatttccggc
     8341 cagttcaacg ccgaactgga aagcatccgc acgcaggtga tgaccatggg cggcatggtg
     8401 gagcagcagc tttctgatgc gatcactgca atgcacaacc aggacagcga tctggcgaag
     8461 cgcgtcatcg aaggcgacaa gaacgtcaac atgatggaag tggcgatcga tgaagcctgc
     8521 gtgcgcatta ttgccaaacg ccagccgacg gcgagcgacc tgcgtctggt tatggtgatc
     8581 agtaaaacca ttgccgagct ggagcgtatt ggcgacgtgg cggacaaaat ctgccgtact
     8641 gcactggaga aattctccca gcagcatcag ccattgctgg taagtctgga gtcgctgggc
     8701 cgtcatacca tccagatgct gcacgacgtg ctggacgcgt tcgcgcggat ggacattgat
     8761 gaagcggtac gtatttatcg tgaagataaa aaagtcgatc aggagtacga aggtattgtt
     8821 cgtcagctga tgacctacat gatggaagat tcgcgcacca ttccaagcgt acttaccgcg
     8881 ctgttttgtg cgcgttcaat cgaacgtatc ggcgaccgct gccagaatat ttgtgagttt
     8941 atcttctact acgtgaaggg gcaggatttc cgtcacgtcg gtggtgacga gctcgataaa
     9001 ctgctggcag aaaaagatag cgataaataa ttcaccaagt aaatcccaat aatttaatta
     9061 ttgggatttg
//
LOCUS       DAZUCC010000196         8800 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZUCC010000196 DAZUCC010000000
VERSION     DAZUCC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.197"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            140..643
                     /gene="ftnB"
                     /locus_tag="ACHY09_004081"
     CDS             140..643
                     /gene="ftnB"
                     /locus_tag="ACHY09_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFS3886979.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(721..972)
                     /gene="yecJ"
                     /locus_tag="ACHY09_004082"
     CDS             complement(721..972)
                     /gene="yecJ"
                     /locus_tag="ACHY09_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFS3886980.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1087..1173)
                     /gene="azuC"
                     /locus_tag="ACHY09_004083"
     CDS             complement(1087..1173)
                     /gene="azuC"
                     /locus_tag="ACHY09_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFS3886981.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1436..1759
                     /gene="yecR"
                     /locus_tag="ACHY09_004084"
     CDS             1436..1759
                     /gene="yecR"
                     /locus_tag="ACHY09_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFS3886982.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1931..2428
                     /gene="ftnA"
                     /locus_tag="ACHY09_004085"
     CDS             1931..2428
                     /gene="ftnA"
                     /locus_tag="ACHY09_004085"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFS3886983.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2466..2705)
                     /gene="yecH"
                     /locus_tag="ACHY09_004086"
     CDS             complement(2466..2705)
                     /gene="yecH"
                     /locus_tag="ACHY09_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFS3886984.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2895..4106
                     /gene="tyrP"
                     /locus_tag="ACHY09_004087"
     CDS             2895..4106
                     /gene="tyrP"
                     /locus_tag="ACHY09_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFS3886985.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4157..4822)
                     /gene="yecA"
                     /locus_tag="ACHY09_004088"
     CDS             complement(4157..4822)
                     /gene="yecA"
                     /locus_tag="ACHY09_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFS3886986.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIESAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALDAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5018..5104)
                     /locus_tag="ACHY09_004089"
     tRNA            complement(5018..5104)
                     /locus_tag="ACHY09_004089"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5068..5070),aa:Leu,seq:taa)
     gene            complement(5117..5190)
                     /locus_tag="ACHY09_004090"
     tRNA            complement(5117..5190)
                     /locus_tag="ACHY09_004090"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5156..5158),aa:Cys,seq:gca)
     gene            complement(5245..5320)
                     /locus_tag="ACHY09_004091"
     tRNA            complement(5245..5320)
                     /locus_tag="ACHY09_004091"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5285..5287),aa:Gly,seq:gcc)
     gene            complement(5472..6020)
                     /gene="pgsA"
                     /locus_tag="ACHY09_004092"
     CDS             complement(5472..6020)
                     /gene="pgsA"
                     /locus_tag="ACHY09_004092"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS3886987.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6077..7909)
                     /gene="uvrC"
                     /locus_tag="ACHY09_004093"
     CDS             complement(6077..7909)
                     /gene="uvrC"
                     /locus_tag="ACHY09_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS3886988.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDESKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7906..8562)
                     /gene="uvrY"
                     /locus_tag="ACHY09_004094"
     CDS             complement(7906..8562)
                     /gene="uvrY"
                     /locus_tag="ACHY09_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFS3886989.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         2157 a         2230 c         2056 g         2357 t
ORIGIN      
        1 attcttattt cagatcatcg tcagaattga ctccacgatc acatttcgga ccggcagaaa
       61 ggaattattc tgcaaacagt aattatggtg ttttgattta tcttgcacct ctccacttct
      121 ggatataagg atattaggta tggcaaccgc tggaatgctt ctcaaactca actctcaaat
      181 gaaccgcgag ttttacgcat ccaatctcta ccttcacctg agtaactggt gttctgaaca
      241 gagtctgaac ggcaccgcca ctttccttcg cgcccaggca cagagtaatg tgacccaaat
      301 gatgcgcatg tttaacttta tgaagagtgt cggcgctacc cccatcgtta aagccattga
      361 tgttcccggt gaaaaactga actctctgga agaactgttc caaaaaacga tggaagaata
      421 cgagcaacgt tctagtacgt tggcacagtt agccgatgaa gcgaaagaac tgaatgatga
      481 ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag cagcatgatg gcctgttgct
      541 gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac
      601 cgaccaacat gttctgaatg tcgtgtcgca tcagctacat tgatcatcat cggcgctaat
      661 gcattgcgcc gatgaagttt ttgagaaacc gctgcctcat ttttttgaag cagcggtttt
      721 ttaatgggat tcgccctgtg gggtaaactt gagttcaatc aacgcgatgg ctttttggat
      781 tgcccgcatg gtgaccgggt ctgcggcggc gggatggtta gtaaagtcga tattctttag
      841 ctggctggac attttttcgc gaacttcaac gggtgcgatc acatcgagaa catccagaat
      901 ttgtttgata accaactggc aagcaaccac atcagaaacc agttcctgat cggcattcag
      961 cggctgggac atcgtaaact cctgatagca ttttgaaagc cgttatagta gcggcttcac
     1021 atcttcagcg atagtcacat ccaccgtcat caggacacaa aaaaacctgc cggagcaggt
     1081 tttttgttat cggaacatat tgcctggcgg tacgtctttg aacgtcttgc aatagttatt
     1141 gaacatactt ttcaggattt tgcgcagttt catcgcggca ctccgaccat ttgttataca
     1201 ggtgttattg tctttgcgct tataatgtga caaccatcac aaaaatcaat ctttatgtga
     1261 tgcaaatcac ataaataccc ctttaatgtt ataaaaatga taatcaaaaa gcagctaccc
     1321 catttctgac acctacagat ggcaagaaat agcgcctgcc aggcgtcttt tccggccatt
     1381 gtcgcagcgc tgtagcgcgt aaaatagtgc tttcttttac tcttctggct ggaccatgag
     1441 acttctgatt ctgactcttt cattaataac gctggcgggg tgtacggtta ctcgccaggc
     1501 ccatgtgagc gaagttgacg cggcaacagg tattgttcgg ttggtttatg atcaggcttt
     1561 tttgcagcat gcccgtaccg atcgttatgt cagtcgcggg atcgccgatc gcgcatgcca
     1621 gcaggcaggc tatacccacg ctatcccctt tggtcagcca gtaggcaact gtagcctctt
     1681 tgcgggttcc ttatgtttga atacggaatt cactttgtca tatcagtgcc atcattccgc
     1741 cttccctgtc tttctctaaa cgttttaatc agcgaggggg atcttcgctg attaaataaa
     1801 tagcggtaat gcgttttatt cccattcgta tttttaataa atgatgttta tattttacct
     1861 tttgcaaata ataaaataac aaattatagt gacgccacag caacaaatat aacctttgtg
     1921 gagcactatc atgctgaaac cagaaatgat tgaaaaactt aatgagcaga tgaacctgga
     1981 actgtactct tcactgcttt atcagcaaat gagcgcctgg tgcagctatc ataccttcga
     2041 aggtgctgcc gcgttcctgc gccgtcacgc ccaggaagag atgacgcata tgcagcgtct
     2101 gtttgattac ctgactgata ccggcaactt accgcgtatt aataccgttg aatctccgtt
     2161 tgctgaatat tcctcacttg atgaattatt ccaggaaacc tataaacacg aacaattaat
     2221 cacgcagaaa attaacgaac tggctcatgc tgcaatgacc aatcaggact acccaacatt
     2281 taatttcctg caatggtatg tttctgagca gcatgaagaa gagaaactgt tcaaatcgat
     2341 tattgataaa ttaagcctgg caggcaaaag tggcgaaggt ctgtatttta tcgacaaaga
     2401 actctctacc ctcgacacac aaaactaatg ctctccggcg gcaggttttc tgccgccgtt
     2461 tcaaatcagt gacggcaaat cttactctga tcggttgaaa aaccttcttc tgaaggtata
     2521 aatttgcctt ttgctgccag aaacgccacc aactctcccg cagtcatccc ttctgccgag
     2581 caggtgtgaa aacgtgcctg ttcaccaaaa cgcgctttaa ttgctgcttc cagactggca
     2641 tgcgtatatt gctcgcctga ttcaatcatc atatttaaca cttcgtgacc gtgaatagag
     2701 tccatcgttc ctcctcaaaa aaagcctagc gtagcgattg ccgcttatga agactttgcg
     2761 ccagcgcaga actgaacgct tttattgtac atttatattt acatcatatg taacgccggt
     2821 ttgacgaatc agccgttatg ccttaacctg cgccgcagat atcactcata aagatcgtca
     2881 ggacagaaga aagcgtgaaa aacagaactc tgggaagtgt ttttatcgtg gcgggaacca
     2941 caattggcgc aggcatgctg gcaatgccgc tggctgcggc aggtgttggt tttagcgtca
     3001 cgttaatctt gttaattggg ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg
     3061 aggtgtacca gcatgtcccg gcagataccg gtctgggcac gctggcaaaa cgctatctgg
     3121 gacgctacgg tcagtggctg acgggcttca gtatgatgtt cttaatgtat gctctgactg
     3181 cggcatacat cagcggtgcc ggtgaattgt tggcctccag catcagcgac tggacaggta
     3241 tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt
     3301 gtgtcggaac ttcgctggtc gatttattta accgttttct gttcagcgcc aaaattattt
     3361 ttctggtggt gatgctggta ttgctgctac cgcatattca caaagtgaat cttttaaccc
     3421 tgccgttgca acagggactg gcactgtctg caatcccggt gatttttacg tcgtttggtt
     3481 ttcacggtag cgtgccgagt attgtcagct atatggatgg caacattcgt aagctacgct
     3541 gggtgtttat aatcggtagt gcgatccccc tggtggcata tattttctgg cagctggcga
     3601 cccttggcag cattgattca acaaccttta tgggattgct ggctaatcat gccggattaa
     3661 acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc gcatgttgag ctggcagtgc
     3721 atttatttgc tgatttagcc ctcgccacgt ccttcctcgg cgtagcgtta ggcttatttg
     3781 attatctggc agatttgttt cagcgttcaa ataccgttgg tggacgattg caaactggtg
     3841 caattacctt tctgccgcca ctggcgtttg cactgtttta tccacgagga tttgtgatgg
     3901 cgctgggtta cgccggtgtg gcgctggcgg tactggcatt gattatccct tcgctgttga
     3961 cctggcaaag caggaagcac aatcctcagg cgggttaccg ggtgaaaggt ggtcgtccgg
     4021 cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg
     4081 caggcttgtt accagaagtg gggtgattag attcttattg ggtgccagaa ttaacgctga
     4141 cacccaattt gacctcttaa tgcaggcagc actgcttaaa tttcttacca ctaccgcacg
     4201 gacaaggatc gttacgcccc ggtttctctt ctgctttgat aggttgctga acagcttttt
     4261 cctgcggatg cgccatccag tatgcatgta aatcaagcgc cgccagtcga atagcatcta
     4321 cgctctcttc aaacgcttct ggcgacatct tctctacccg ctcgaagttt tcctcagtac
     4381 cgtgcagcgc aatcgcatcc agcgctggtt ttaacgaatc gggcaacgtt gaccagtcag
     4441 aaagtgccac gccccgcata tagccaaagc accactcctc aacaatcgtc agctcgctgc
     4501 catcgacttc tcgcaagccg aataacggct caaactgctc cgggaattcg ttcagacgct
     4561 ctgcggtatc agccatatgt tgaaaagcca ggttcataaa gcgcgtcatc tctttctctg
     4621 acgcccagcg cggcacatag tcagccccac cccacacggc aaccagccac tgtgccgatt
     4681 caatctcttg cggagaactc aacaccgccg tcaataaacc gtccagctcc gccacatcaa
     4741 gaatggcgtg gtcagtgttg tatttggtca gaatatcgtc cagccattcc aactcacttt
     4801 cgtttaacgg tcccgttttc atacgctttt ccttgtggat ctcaactcgc cagcacctat
     4861 cttacatgcc ggtccgtatc agagatactt tttgagtggc tttgccggtg attaaaaatt
     4921 aaggagggtg taacgacaag ttgcaggcac aaaaaaacca cccgaaggtg gtttcacgac
     4981 actgcttatt gctttgattt tattcttatc tttcccatgg tacccggagc gggacttgaa
     5041 cccgcacagc gcgaacgccg agggatttta aatcccttgt gtctaccgat tccaccatcc
     5101 gggctcggga agaaagtgga ggcgcgttcc ggagtcgaac cggactagac ggatttgcaa
     5161 tccgctacat aaccgctttg ttaacgcgcc aaattcttca ggcctttcag ccagacatcc
     5221 gcttgacgcc gatgtctttt aaactggagc gggaaacgag actcgaactc gcgaccccga
     5281 ccttggcaag gtcgtgctct accaactgag ctattcccgc attcatcaag caatcagtta
     5341 atcacttgat tttattatcg tctggcaatc agtgccgccg ttcgatgcgt tgcattctac
     5401 ttacctggcg cgatgagtca acgatatttt tcaccacttt tgatcgtttg ctgaaaattg
     5461 cgccgaaacg atcactgatc aagcaaatct gcacgcgcag cgctcaaata ttgcaacatt
     5521 gaccacagag tcagtaccgc agccacaaag aaaagtgcaa taccggcgta ctcaacccaa
     5581 atgttcggac gccacagcag ccaggccagc gccaccatct gggcagtggt tttcactttc
     5641 ccaatccagg agacagccac gctactgcgt ttacccaact ccgccatcca ttcgcgtaac
     5701 gcagaaataa taatttcacg ggcgatcatc gttgctgccg gtaaggtcac ccaccagctg
     5761 tgataatgct cggttaccag caccatggcg atagccacga gaactttatc tgctacaggg
     5821 tcaaggaaag caccaaaccg ggtactctgg ttccagcggc gtgccagaaa accatcgaac
     5881 cagtcagtca ccgccgcgac gcagaaaatg agcgcggcgg caaacggcga ccaggtgaca
     5941 ggcagataaa agaccaatac aaagaatggg ataaggatga cacggaacag tgtaagcaac
     6001 gtagggatat taaattgcat aatgacgggt aactatctgt tgtcagtaag attaccccta
     6061 tgttgctaca gagacatcaa tgtttcaacg accagaagat cttttctgcc agaccttgcg
     6121 aaatacccgg cacttttgca atttcctcga cgctggcgtt acgtaaacct tgcaaaccgc
     6181 ccatatattt caacaacatt tgccgacgtt ttggcccgac gccttcaatg gtttccaggg
     6241 aactggtatt tttgaccttc gcccgttttt tacggtgccc gccaatcgcg tgatcgtgtg
     6301 attcatcgcg aatatgctgg ataacatgca gtgcgggaga atctggcggc aaactgaatc
     6361 cctcaccttc cggctcaaag aacagcgttt ccagcccagc cttacgatct gctcctttgg
     6421 caacgcctag tagcagcgga tgatttttat cccatgagac atccagttcg gcgaaaacat
     6481 ttttcgcctg cgcaagttgg cctttgccgc cgtcgataag gatcacatcc gggatcttac
     6541 tctcgtcgat ggctttacca taacgccgac gcagcacctg attcatcgcc gcataatcat
     6601 cgcccggcgt gatgccagta atgttatagc gtcgatactc cgcacgcagc gggccgttag
     6661 catcaaacac cacacaggaa gcgacggttt gttcccccat ggtatggctg atgtcaaagc
     6721 attccatccg cttcacttcc ggcaatttca acacgctggc aagcgcggtc agtcgctggt
     6781 gaacggtaga ttgctgcgaa agtttgctgg ttaaggccgt cgccgcattg gtgcgcgcga
     6841 gtttcagata acgcgcccga tcgccgcgag gtttggtttg aacattaatc ttgcgtcccg
     6901 ccagttctga aagggaatcg gcgagcagcg ttttatcgct aagattaaaa tcgagcagga
     6961 tctcacccgg taaggtgcgc atctggctgc cttgtaaata gaactgacct acgaaggttt
     7021 ccaccacctc gctcagttcc gtaccgccag gcactttcgg gaaatagctg cggctgccga
     7081 gcactttacc ctgacgaatg aacaatacgt ggacacaagc catgcccgca tcgaacgcca
     7141 caccaataac gtcgaggtcg tcgccggtat tggacacaaa ctgtttttcg gtgacgcgtc
     7201 gcaccgcctg aatttggtcg cgaatacgtg ccgcttcttc aaactccaga ttctggctgg
     7261 cagtttccat acgactaatg agttgcgtaa gcacctgatc atctttgcca gacaaaaaca
     7321 ggcgcacata ctcgacctgc tgagcgtatt cttcttcact caccagtcct tcaacgcacg
     7381 gtcccagaca gcgccctatc tgatattgca gacacggacg cgagcgattg cgataaacac
     7441 tattttcgca ctggcgaatg gggaatatct tttgcagtag cgccagtgtt tcacgtacgg
     7501 catagccatt cgggaacggg ccgaaatatt cacctttagc atgcttcgca ccacgatgca
     7561 tcgccagacg cggatgggta tcgccactca ggaagataaa gggataagat ttatcatcgc
     7621 gtagcaaaac gttgtaacgc ggctgataga gtttgatgta gttgtgttcc agcaacagcg
     7681 cttcggtttc tgtatgagta accgttacat caatttgctg gatctgggcg accagcgctt
     7741 cggttttgcg cgaagcgagg ttgctacgga aatagctgga aagccgtttt ttcaggtctt
     7801 tcgctttgcc gacatagata accgtaccac cagcatcgta catgcgataa acgcctggct
     7861 ggctggttac ggtttttaaa aacgcttttg cgtcaaactg atcactcact gacttgataa
     7921 tgtctccgca ttacacagac catggcgaat tgccaggtga gtcagctcaa catcgccatg
     7981 aatgtttagt ttactgaaca tacgatagcg gtagctgttc accgttttcg gactgagatt
     8041 gagctgttct gagatctcat tgaccttctg gcccttggtg atcatcagca taatctgcaa
     8101 ttcacgttca gacaaactgg caaatgggct ttctgttttt tctggttcga tctggcttaa
     8161 cgccatttgt tgagcgatgt cagaagcaat gtaacgctgc cctgaataga cagaacggat
     8221 cgcactcaca acttcctgcg gagccgcgcc tttgctgagg tagcccgcag caccggcctg
     8281 catgactttc gctggtaaag ggttttctgt atggacggta agcatgatga ttttaacatc
     8341 agctgtggaa cgcgcgattt tacgcgtcgc ctcaaggccg ccaatgcccg gcatactcat
     8401 gtccattagc accacgtcaa cggcatttgc ccggcaccat ttaacggcgt cttcaccgca
     8461 cgatgcctct ccgacgactt ttataccctt tatatcttcc agaatgcgtc gtatccctgc
     8521 gcgcaccagt tcgtggtcat caacaagtag aacgttgatc aaaggaatat ctccagaaat
     8581 agggataacg ctactgatag ttagtcattc gtatattagc ggtttttatt gcaactttga
     8641 aacgttaaaa atggtgggta ttcgattttt ctctcgtttt tgtaaattcg attgtccaca
     8701 gttatggtca cgcccgtcac caacggcttc cgggatcgtt tcactgtaag ccctgcgatg
     8761 aaatgtgaca aaaattgact ttattcagca aaaatgaaaa
//
LOCUS       DAZUCC010000197         8749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZUCC010000197 DAZUCC010000000
VERSION     DAZUCC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.198"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..261
                     /locus_tag="ACHY09_004095"
     CDS             <1..261
                     /locus_tag="ACHY09_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886990.1"
                     /translation="FSELTEIAINEYKDSRFVSFGLYCETTSPYIHAASEIVDSHIDP
                     QRIQELTLALQLLRENGEYNCSDCQHEKKKTTHKTTNQATLH"
     gene            370..2448
                     /locus_tag="ACHY09_004096"
     CDS             370..2448
                     /locus_tag="ACHY09_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFS3886991.1"
                     /translation="MSADKQTFALHCEAKNDKVRKRLGIKGGFFWADTRKLSVAVSRC
                     IAAMDDAGYDEDDFKKPVRVNFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMMAIPFD
                     DNINGEDATTAGDDNDNLDGTIPDDVEKSESPDSDDDCSECEIPVATLSLTHRFLHLF
                     FFSKDEDGKYRHHATPEQRNNVIRMEMDTEDSYLQSLLTAVRSHPELDKLTNYHLGRL
                     AQSVGKAFSHATPRHISPAEFDKFISTWMKTDYVDQGLLAKEWQKGNYVTGITRTPSG
                     ANAGGGNLTDRGEGFTHNQASLARDIATGVLARSMDVDIYNLHPAHAKRVEEIVAENK
                     PPFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEAIPARVTEYLNKVLTETDHANP
                     DPEIVDIACGRSSAPMPQRVTKEGKQDDEEKPQPSGAIADEQATTETVEPDTTEHNQD
                     TQSMDAQPQINSVDAKYQKLRAELYEARKNIPPKNPVDADKLLAASRGEFVEGISDPT
                     DPKWVKGIQTRDTEDQNQSKVEQNGPEAVQNSPDTQQNGPEEKQPGPVTQQEVEKVCT
                     ACSQNGGGHCPDCGSVMGDNTYTDTFGENDAADGEDSAQTADKNIQENSVDTVQVHET
                     VVQNEPCRDTSGDEADYESVSLDWKRQLVIAAVYGLCANPACIATAPAIPDIAIMIAN
                     RLEHFGGDKS"
     gene            2445..2741
                     /locus_tag="ACHY09_004097"
     CDS             2445..2741
                     /locus_tag="ACHY09_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFS3886992.1"
                     /translation="MNAWLVPDRIEEQSWAQHYRKIAREETEAELADDLEKGLPQRLF
                     ESLCIDNLQRCGASKQAITRAFDDDVDFQERMAEHIRYMAETIARHQINIDSEV"
     gene            2747..3532
                     /gene="bet"
                     /locus_tag="ACHY09_004098"
     CDS             2747..3532
                     /gene="bet"
                     /locus_tag="ACHY09_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFS3886993.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRRAPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNGETMQEINGLLTSMDKTWDD
                     DLLPLCSQIFRRDIRALSELSQAEAEKALGFLKQKATEHKVAA"
     gene            3529..4209
                     /locus_tag="ACHY09_004099"
     CDS             3529..4209
                     /locus_tag="ACHY09_004099"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFS3886994.1"
                     /translation="MTPEIILQRTGIDINNVEQGDDAWQKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVASEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQF
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIS
                     FVFGEQWR"
     gene            4234..4467
                     /locus_tag="ACHY09_004100"
     CDS             4234..4467
                     /locus_tag="ACHY09_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3886995.1"
                     /translation="MNISFESHGSAASVVITSSLFECRKHQHIVDALKFKVPEMTVTT
                     RGCFWIRTTLTTTYLTARRVYDMAHQEYNQCSS"
     gene            4455..4706
                     /locus_tag="ACHY09_004101"
     CDS             4455..4706
                     /locus_tag="ACHY09_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS3886996.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERVDHNELHMIKAEIEKEMHIASLRKMRRTSIN"
     gene            4752..5036
                     /locus_tag="ACHY09_004102"
     CDS             4752..5036
                     /locus_tag="ACHY09_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFS3886997.1"
                     /translation="MRELVNQYSHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDTAWMNGREYKQIAPDGTPKKNSECLYHLPTINNWIKNQPLPSQDV"
     gene            5089..6399
                     /locus_tag="ACHY09_004103"
     CDS             5089..6399
                     /locus_tag="ACHY09_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3886998.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYADRFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSRKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLGRQHHITVKLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNVLNARAPDVPQSDQEDEIKLYFSK"
     gene            6396..6974
                     /gene="wrbA"
                     /locus_tag="ACHY09_004104"
     CDS             6396..6974
                     /gene="wrbA"
                     /locus_tag="ACHY09_004104"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFS3886999.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNS"
     gene            6995..7222
                     /locus_tag="ACHY09_004105"
     CDS             6995..7222
                     /locus_tag="ACHY09_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFS3887000.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7260..8501)
                     /gene="agp"
                     /locus_tag="ACHY09_004106"
     CDS             complement(7260..8501)
                     /gene="agp"
                     /locus_tag="ACHY09_004106"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFS3887001.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
BASE COUNT         2481 a         2171 c         2129 g         1968 t
ORIGIN      
        1 ttttcagagc taacagaaat cgcaattaac gagtataagg acagtcgctt tgtttccttc
       61 ggtttatatt gcgaaaccac cagcccatat attcatgctg catctgaaat tgtcgacagt
      121 catattgatc cacaaagaat tcaggaactg accctggcac ttcaacttct cagagaaaat
      181 ggcgaataca actgctctga ctgccagcat gaaaagaaaa aaacaactca caaaacaaca
      241 aaccaggcaa ccttacactg aaataaaaaa tccggcagcg caggctaccg gactaccccc
      301 tgcgtcactg tattcggaga aatcagacaa agggccgcta attctaatcc agccagaggt
      361 ttaaatacaa tgagcgctga taaacaaact tttgcactgc actgcgaagc aaaaaacgat
      421 aaagtcagaa aacgccttgg catcaaaggc ggttttttct gggctgatac cagaaaactt
      481 tctgtcgcag tttcccgctg cattgcagcc atggacgatg caggctacga cgaggatgat
      541 ttcaaaaaac ccgttcgcgt aaatttcccc gtcgtgaatg accttccacc ggaaggcgtg
      601 tttgatactg aattctgcaa ccgctatgaa aaaggcgggg aagatggcat caccatgatg
      661 gctatcccct tcgatgacaa catcaacggt gaagatgcca caactgctgg cgatgacaac
      721 gataacctgg acggaactat tccggatgat gtggagaaaa gcgaatcccc ggacagcgac
      781 gatgactgtt ctgaatgtga aattcccgtc gccactctga gccttactca tcgcttcctt
      841 cacctcttct tcttcagcaa ggatgaagat ggaaaatacc ggcatcacgc cacacccgaa
      901 caacgaaata acgtgatccg tatggagatg gacacagaag acagttacct tcagagcctg
      961 cttactgctg tgcgttcaca tccagagctg gataagctga cgaattatca tctgggaaga
     1021 ctggcacaat ccgttggaaa ggcattctct cacgcaacac cccgtcatat cagcccggcc
     1081 gaattcgaca agttcatctc cacctggatg aaaacagact atgtggatca gggattgctg
     1141 gcaaaagaat ggcagaaagg aaattatgtt acaggcatca ctcgtacgcc ttccggtgct
     1201 aacgctggcg gcggaaatct taccgatcgt ggcgaaggct ttacccacaa tcaggcatca
     1261 ctggcgcgag acattgccac tggcgttctg gcccgttcaa tggatgtgga tatttataac
     1321 ctgcaccctg cacatgcaaa acgcgttgaa gaaatcgttg cagagaataa accacccttt
     1381 tctgttttcc gtgacaaatt catcgccatg cccggtgggc tggattattc ccgcgccatt
     1441 gtggtggctt cagtgaaaga agcaccaatc ggcattgagg ctatcccggc gcgcgtgact
     1501 gaatatctca acaaagtgtt gaccgaaacc gatcacgcta accctgatcc agaaatcgtg
     1561 gatattgcct gcggtcgctc ctctgccccg atgccgcagc gtgtaacaaa agaaggaaaa
     1621 caggatgatg aagaaaaacc acagccatct ggcgcaatag cagatgaaca ggcaacgact
     1681 gaaacagtgg aaccggatac aactgaacat aatcaggaca cgcagtcgat ggatgctcag
     1741 ccacagataa attctgttga tgcgaaatat caaaaactgc gtgcagaact ctatgaagcc
     1801 aggaaaaaca ttccacccaa aaatcctgtc gatgcagaca aattgctggc tgcctctcgt
     1861 ggggagttcg ttgaaggtat cagcgaccca accgacccga agtgggtgaa ggggatccag
     1921 actcgcgaca ctgaggacca gaatcaatcc aaagtggaac aaaatgggcc agaagcggta
     1981 caaaacagcc cggatacgca acaaaacgga ccagaagaga aacagccagg gccagtaacg
     2041 caacaagaag tggaaaaggt ttgcaccgca tgcagtcaga acggtggcgg gcattgccct
     2101 gactgcggtt cagtaatggg cgataacact tacacagata cttttggaga aaatgacgcc
     2161 gctgatggag aagactcagc gcaaacagcg gataagaaca ttcaggaaaa ttctgttgat
     2221 accgttcagg tgcacgaaac cgttgttcag aacgagccct gcagagatac gtccggcgat
     2281 gaggccgatt atgagtcagt aagtctcgac tggaaaagac agctcgtgat tgccgccgtc
     2341 tatggtttat gcgccaaccc cgcatgtatt gccacagcgc cagcaatccc tgatatcgcc
     2401 atcatgattg ccaacaggct tgaacatttc ggaggtgata aatcatgaat gcttggcttg
     2461 tccccgatcg cattgaagag cagtcatggg cacaacacta ccggaaaatt gcccgtgaag
     2521 aaactgaagc agagctggcg gacgacctgg aaaaaggact gccccagcgc ctgtttgaat
     2581 cgctctgcat agataaccta caacgctgtg gcgccagcaa acaggccatt acccgagcat
     2641 ttgatgacga tgtcgatttt caggaacgca tggcagaaca catccgctac atggctgaaa
     2701 ccatcgcccg tcaccaaatt aatattgatt cagaggtata aaacggatga gtacagcact
     2761 cgcaacgctg gcagggaagc tggctgaacg tgtcggcatg gattctgtcg acccacagga
     2821 actaatcacc actcttcgcc agacggcatt taaaggtgat gccagcgatg cgcaatttat
     2881 cgcattgttg atcgtcgcca accagtacgg ccttaatccc tggacgaaag aaatttacgc
     2941 cttccctgat aagcagaacg gcatcgttcc ggttgttggc gttgatggct ggtcccgcat
     3001 tatcaatgaa aaccagcagt ttgatggcat ggactttgag caggacaatg agtcctgtac
     3061 atgccggatt taccgcaaag atcgcaatca cccgatctgc gttaccgagt ggatggatga
     3121 atgtcgccgc gcaccattca aaacccgcga aggcagagaa atcacggggc cgtggcagtc
     3181 gcatcccaaa cggatgttac ggcataaagc gatgattcag tgtgcccgtc tggccttcgg
     3241 atttgctggt atctatgaca aggatgaagc cgaacgcatt gtcgaaaata ccgcatacac
     3301 agcagaacgt cagccggaac gcgacatcac cccggttaat ggtgaaacca tgcaggaaat
     3361 taacggtctg cttacctcca tggataaaac atgggatgac gacttattac cactctgttc
     3421 ccagatattt cgccgcgaca ttcgagcgtt gtcagaactg tcccaggccg aagcagagaa
     3481 ggctcttgga ttccttaaac agaaagccac agagcataag gtggcagcat gacaccagaa
     3541 attatcctgc aacgcacagg tattgacata aacaatgtcg aacagggaga tgatgcatgg
     3601 caaaaattac ggctcggcgt catcacagct tcagaagttc acaacgtgat agcaaaaccc
     3661 cgctccggaa agaaatggcc tgacatgaaa atgtcctact tccacaccct gctggctgag
     3721 gtctgcacgg gtgtggcctc ggaagttaac gccaaagccc tggcatgggg aaaacagtac
     3781 gagaacgacg ccagagccct gtttgagttt acttctggcg tgaatgttac tgaatccccg
     3841 atcatctatc gcgacgaaag tatgcgtacc gcctgctctc cggatggatt atgcagtgac
     3901 ggcaacggcc ttgagctgaa atgtccgttt acctcccggg atttcatgaa attccggctc
     3961 ggtggtttcg aggcaataaa atcagcttac atggcccagg tgcaattcag catgtgggtg
     4021 actcgaaaag atgcctggta cttcgccaac tacgacccgc gcatgaagcg tgaaggcctg
     4081 cattatgtcg tggtcgagcg ggatgaaaaa tacatggctg gttttgacga gatggtgccg
     4141 gagttcatcg aaaaaatgga cgaagcactg gctgaaatta gttttgtatt tggggagcaa
     4201 tggcgataac cagtaacaat gaggttctca tatatgaata tttcatttga aagtcatggt
     4261 tcggcagcaa gtgttgttat caccagttcg ctattcgaat gccgaaagca ccagcatatc
     4321 gttgatgcac tgaagttcaa agtaccggaa atgaccgtca caacccgggg atgtttctgg
     4381 atacggacga cgttaaccac aacatacctc acggcacgcc gtgtttatga tatggcacac
     4441 caggaatata accagtgttc gtcctgatcc agcgcgggca gtctttcgtc gatgccaaca
     4501 actatccggt ggaaatctgc aaggtaactc ttactcaggt gatctaccga aggctcgacg
     4561 gcagaacccg agccacttca attggtgcat ttaatgaaga atttgagcga gtcgaccaca
     4621 acgaactaca catgattaaa gcggaaattg agaaggaaat gcatattgcc agccttcgaa
     4681 aaatgcgccg cacatcaatc aactgacaac cgccttcggg cggttttttc ttacacatgc
     4741 ggaggcaact tatgcgcgag ttagtaaacc agtatagcca tggcattcag ccagtcatca
     4801 cacctgttgt acaaataaat gcgaatgaat gggtaacact ggagctttta atggctgtaa
     4861 caggcctgag aaaaggaacc atattacgcg ccagggacac tgcgtggatg aacggcagag
     4921 aatataaaca aatcgccccc gacggaacgc caaagaaaaa cagcgaatgc ctctatcatc
     4981 ttcctaccat caacaactgg atcaaaaacc aacctctacc atctcaggat gtttaattct
     5041 tgtccataag agtataacct gaaagtgctc ttggacgcag gaggaacaat ggcgaattca
     5101 gcctatccag ccggcgttga aaatcacgga ggaaaactcc gaataacatt taagtacagg
     5161 ggtaaacgag tgcgcgaaaa tcttcgcgtg cccgatacac cgaaaaacag aaagatcgct
     5221 ggtgagttaa gggcttcggt ctgctttgca atcagaacag gaacgtttga ttatgccgat
     5281 cgattccctg actcacctaa cctgaagcta tttggcctgg taaaaaaaga tatcaccgtc
     5341 ggtgaactgg cacagaaatg gcttactctg aaagcaatgg aaatcagtag taacgcctta
     5401 aatcgttatc aatcagtgat gaaaaatatg ctaccgaggc ttggtcctgg caggctggcg
     5461 tcatcgatta caaaagaaga tctgctgttt atcaggaaag atttactgac cggggaaaag
     5521 ggaagcagga aaaccagcac gtcccgaaaa ggaagaaccg tacccacagt gaactattac
     5581 atgacaacaa cagccgggat gttcagcttt gccgccgaaa acgggtatct ggagaaaaac
     5641 ccatttaatt caataacacc actgaggaaa tcaaaaccag tgccggatcc actgaccaga
     5701 gatgagttta gtcgtctcat tgatgcctgc catcatcaac agaccaaaaa cctctggaca
     5761 gtggctgttt ttacaggaat gcgacacggt gaaattgccg cacttgcatg ggaggatatc
     5821 gacctgaaag ctggcacgat aacagtgcga cgaaatttta caaaaatagg tgattttacg
     5881 ctaccaaaga ccgacgcagg cactaaccgg gttatacatc ttctggcacc agcaattgaa
     5941 gcacttaaaa accaggcgat gcttactcgt cttggcaggc agcatcacat caccgttaaa
     6001 ttgcgcgagt acggaagaac aattttgcac gagtgcactt ttgttttctg tccgcaaatc
     6061 gttcgcaaga atcacaaggc gggtattaac tacgcggtaa gctccatcgg agcgacatgg
     6121 gattcagcaa taaaaagagc gggtatcaga tcccgtaaag cgtatcagtc acgccatacc
     6181 tatgcgtgct gggctttatc tgccggagca aacccgacat ttattgcatc acagatgggg
     6241 cactccagcg ccagcatggt ctacaatgtt tatggtgcat ggatgcctga gtgcagcgtg
     6301 actcaggttg ccatgttgaa taatgtcctt aatgcccgtg ccccagacgt gccccaaagt
     6361 gaccaggagg atgaaataaa attatatttt tcaaaatgat aaaccatatc cttagacatg
     6421 ttgaaacgat ggcacgcgca gttgctgagg gtgcaagcaa agtggatggc gcagaagttg
     6481 tcgttaagcg tgtaccggaa accatgccgc cgcaattatt tgaaaaagca ggcggtaaaa
     6541 cgcaaactgc accggttgca accccgcaag aactggccga ttacgacgcc attatttttg
     6601 gtacacctac ccgctttggt aacatgtccg gtcaaatgcg taccttcctc gaccagacgg
     6661 gcggcctgtg ggcttccggc gcactatacg gaaaactggc gagcgtcttt agttccaccg
     6721 gtactggcgg cggtcaggaa caaaccatca catccacctg gacgaccctt gcgcatcacg
     6781 gcatggtgat tgtccccatt ggctacgcag cgcaggaatt atttgacgtt tcacaggttc
     6841 gcggcggtac gccgtacggc gcaaccacca tcgcaggcgg tgacggttca cgccagccca
     6901 gccaggaaga actgtctatt gctcgttatc aaggggaata tgtcgcaggt ctggcagtta
     6961 aacttaacag ctaatcttca acaggaggat acgcatgcca actcaagaag cgaaagctca
     7021 tcacgtcggt gaatgggcat ctctgcgcaa tacgtcgccg gaaatagccg aggccatttt
     7081 cgaagtcgcc gggtacgacg aaaaaatggc ggaaaagatt tgggaagaag gtagcgatga
     7141 agtgttagtc aaagcctttg ctaagaccga taaagattcg cttttttggg gcgaacagac
     7201 catcgaacgt aaaaacgttt aaccagcgac tcccccgctt cacgcggggg agttttctgt
     7261 tatttcaccg cttcattcaa tacgcgatca aacttatcca ttgggcaaaa accgttggca
     7321 tctatcgggc aaccgcttaa ttccagcgtc acgcgctgcg caggtgcctg cagggttaac
     7381 gcatcggcat tacgtaactg ttccgcactc tggtacacat attcaatttt catcaaatcg
     7441 cgattggctt tgctgtcatg ccaacgctgg aaaacgattt tgccgccaat cggcgtgcgt
     7501 tcgttctggt catgcaactg atacggtttg aaatccagcg ccgttaacag agaggcaatg
     7561 ttggagtcgt gcccaactaa taccgtgatt ttcggtgcac tggtgcgatc ggtgaccagg
     7621 gctttgtcga tataactgac cagcggtttc gcaacattgc gcgccacttc cggtgaggta
     7681 aacaggctgt cctggtaacc gtttttcagc ttcgacaaca ccttccactg ctggtcagat
     7741 ttgatttctc cccaggccac ctgatccatc ggaaaacctt cgtagtattg caaagtaaac
     7801 gcatctacca gcgagttgcc gactttcagc ggcccggaaa cgcccggttc ttgttgatac
     7861 ttcgcgctaa aggtattttt gccatctaca agcgaacatt gttgcttctc tttacacgca
     7921 ggagaatctt tatagttaac gatttgttcc agtagcttgt agctgtcggt aagctggagt
     7981 ttgctgagct ctttctccat tgccgccacc gcctgttcac tgaatgcggc ggaatcatcg
     8041 gtgatcactg ggttaaaggt ggggtccata gtgcccattt tttcctggtg atgaacagga
     8101 atatcacacc cagggaatgc gccagtaata aagaactgcg cggtcgcgac ggtgcgttgc
     8161 agactattgg cataggcata aacggtgtcc ggtggcgggc attcccctga tttcaccatc
     8221 ccctgttctg ccagccattc acgcatataa tggcccatat acacttcgag cacgccccct
     8281 ttagtggtga gctgcccacc ggggacgtcc cattcaggcc atttattcgg cgtcgactgc
     8341 tccagcacac tgccattgtt cgccagcggc gcacgtaagt tatgacggct catcataagg
     8401 acttgctgta gctgatagcc ttccggtacg gtttgtgcct gagcatttga agcgagtaaa
     8461 actatccctg ccacagttgc ggcgattagc gttttgttca ttcctcacac ctcttttgtt
     8521 atcaataatt aaagagtgtg acagaaatat gtcatttttc cgggaacagg ttcgcaaaat
     8581 ggctcgttga acgcctgttt ttctatattc tcctttaaaa acaggcaagt tttgacgcaa
     8641 actatgcctg attagccccg gcctcgttta cgccgctaat gattacatca gtagctataa
     8701 agaaaatcag cggcgttacc attaccaata aaaaaagaat gtgagacta
//
LOCUS       DAZUCC010000198         8657 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZUCC010000198 DAZUCC010000000
VERSION     DAZUCC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.199"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1272)
                     /locus_tag="ACHY09_004107"
     CDS             complement(<1..1272)
                     /locus_tag="ACHY09_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3887002.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQ"
     gene            complement(1306..1560)
                     /gene="xisR"
                     /locus_tag="ACHY09_004108"
     CDS             complement(1306..1560)
                     /gene="xisR"
                     /locus_tag="ACHY09_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFS3887003.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(1568..1714)
                     /locus_tag="ACHY09_004109"
     CDS             complement(1568..1714)
                     /locus_tag="ACHY09_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887004.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(1735..1977)
                     /locus_tag="ACHY09_004110"
     CDS             complement(1735..1977)
                     /locus_tag="ACHY09_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887005.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKARAPAQPNELA"
     gene            complement(1970..2872)
                     /locus_tag="ACHY09_004111"
     CDS             complement(1970..2872)
                     /locus_tag="ACHY09_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887006.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(2869..3093)
                     /locus_tag="ACHY09_004112"
     CDS             complement(2869..3093)
                     /locus_tag="ACHY09_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887007.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            complement(3110..3472)
                     /locus_tag="ACHY09_004113"
     CDS             complement(3110..3472)
                     /locus_tag="ACHY09_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS3887008.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEDLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(3465..4331)
                     /locus_tag="ACHY09_004114"
     CDS             complement(3465..4331)
                     /locus_tag="ACHY09_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFS3887009.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGITVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(4355..5023)
                     /locus_tag="ACHY09_004115"
     CDS             complement(4355..5023)
                     /locus_tag="ACHY09_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887010.1"
                     /translation="MPGWKNTTPGLISIAGLSRKDHLRREQTMTATEQLFFLTAEGIH
                     TPYGLLDINKALEMLDEGCFDEPIWHGLEIVCCISEAEEKGMTGELAPEKTIMYWRWL
                     YTAMFFSEQLTRNGWTEVLNENGGTDKGTMYRGHNGGMTIYLKPLRLAMQNYMEWALI
                     ETYGAESGLLKSILMYKAFTGALGDGEKTGITMEGYRAMHMILDGLISEATDNSEPET
                     RVFH"
     gene            complement(4926..5486)
                     /locus_tag="ACHY09_004116"
     CDS             complement(4926..5486)
                     /locus_tag="ACHY09_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887011.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQCLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            complement(5894..6256)
                     /locus_tag="ACHY09_004117"
     CDS             complement(5894..6256)
                     /locus_tag="ACHY09_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887012.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGQKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITRCVNH"
     gene            complement(6431..6631)
                     /locus_tag="ACHY09_004118"
     CDS             complement(6431..6631)
                     /locus_tag="ACHY09_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887013.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCMIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(6628..6819)
                     /locus_tag="ACHY09_004119"
     CDS             complement(6628..6819)
                     /locus_tag="ACHY09_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887014.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(6821..7198)
                     /locus_tag="ACHY09_004120"
     CDS             complement(6821..7198)
                     /locus_tag="ACHY09_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3887015.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(7284..7943)
                     /locus_tag="ACHY09_004121"
     CDS             complement(7284..7943)
                     /locus_tag="ACHY09_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFS3887016.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            8283..8576
                     /locus_tag="ACHY09_004122"
     CDS             8283..8576
                     /locus_tag="ACHY09_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887017.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
BASE COUNT         2014 a         2018 c         1943 g         2682 t
ORIGIN      
        1 ttggggcacc tgtgggacat aagtacatag ctttctgttt agtacttcta cctgggactg
       61 gttattgtct ttcatccatg caccgtaaac attgtaaacc atctgggcat tcgcatgtcc
      121 catctggttg gctatgaagt ttggatttgc tccggcagtt aaagcccagc aagcataagt
      181 atgccgagac tgatacgctt ttctatggcg aatttttgct cgttttaatg cagattccca
      241 tgttctgttg atggatgtta cggcataatg aactcctgag ttacctgtgc gatctaatat
      301 ctgaggattg aaaacaaagc tgcatgagtg agtgcttgtc cggccatact ctcgcagctt
      361 tacttctatc tgatattgtt taccaagcct ggttaatgtc gcctgatttt ttaatacgtc
      421 aattgctggc tgtatgagtt gtattaccct gtttgttcct gcatctgttt tgggtagtgt
      481 aaattccttt gtctgagtgt aattcctttt gacggtcaag gtacccgctt ccagatcgat
      541 atcctcccat gccagcccac acagttcgcc atgccgcatc ccggtatata cagccagcga
      601 ccagaaattt ttagtttgct ggtggtggca cgcatcaatc agtctggcaa actcatcttt
      661 tgttagtgga tcgggaggtg ttttcgctct tttcagtgat ggaagctcac tgaaagggtt
      721 tttgcctata tacccattgt ttgccgcaaa acggaacatc ccggagataa ttgtcatata
      781 gctatttaca gtaggtacac tacgaccttt aataggcttc ctctgatttt ttcctggcaa
      841 ctgatatccc gttaataatt ctttacgaaa aatgagaaca tcttcctgtg taattgatga
      901 tgccagtttg ttccctccaa ggcagtcaag gcaatttcgg atagctgatt tgtatctgac
      961 catggcattt gctgttattt ccattttctt aagctcaagc catttttccg aaagtgcctt
     1021 aatggttatc tctttttttc ccagaccaaa atgtttcagg ttaggggaat tagggaactg
     1081 cgcggcgtag tcgaagctcc ccattcttat tgcaaaacaa acggaagtgc gaagttcacc
     1141 tgcgatcttc cggtttttgg cggtgtcagg aacaccgagg ttttctctga cacgtttgcc
     1201 gttatagtga aaccatatgc ggagtgatcc gccatggttt tcaacgcctg tcgggtatga
     1261 tgcgttactc attaaacctc ccagacgtcc aggagcatta acaggttaac cggaacttgc
     1321 atttttggca cctggttgtt tctggttttc gatccagcgc ataatttcct caatgttgta
     1381 tacacattca ctgaaaggcc cgggctctcc ttctgctgcg taatgacggt attctttccc
     1441 cagtaaccag gatttttttc ttgctctggc aatggttcct gatttcagtc cagttgcagc
     1501 aataagggtt ctttctgtgc accatttgct gggcgttatc tgatagatga ttgtttccat
     1561 tcctgactca cactatcttc aggccacggc agtggcacca cacgtcaaac attcgcttta
     1621 caacttcacg gcagtagaag ccgtgagcat ctctggttaa atcgtatcgg tacccgtatc
     1681 tgagtcgtat ccatatttca aactcgcgat tcacgactta ttctcctgct gtctttatgc
     1741 cagttcattc ggttgcgctg gggccctggc cttccgtggc ctgtatttgc ggcgttttct
     1801 ggcttcaatg cgtatctttg tcaccctgac tggctttggt tcgtgccgga acactttgtt
     1861 tctgatatta cgccgtgtat tcatcatcca gataaaacgc tgggtatggt cgcatccgtc
     1921 atcaacggta attactgtgc ggaccagttc atcgttaata tcaaccattt cacccattca
     1981 ctgagccttc atacgcgaat ttcgtggctt ccatcagttg cgtccatacc cattcaagaa
     2041 attgtgcgtt ttctcctgag cggtcatatt catcgacgct gcggggcttg atggtcgcca
     2101 gtagtggcac aatccggtac ttcatctgtt cggttaactt gtccggtaca agtcgccagc
     2161 cgttatattt cgatggaaca tggtggtatt cagccaggaa gcgtcgtata tcgtttttca
     2221 tttcctcgca tgagttctga tcatgaagtg cagcggaaag ccagcgtccg atatattcag
     2281 ggattcgcac atcaagtact ttttctgccg tcgtcagcat tgcctgtcgg caggcgttcc
     2341 agccgttgac atagtgacgt gttgcatccc atgaaacctg cacatcaggg taagcatcct
     2401 gttctgttct cactggcggt atctgaggaa cagtgaatag tggactgacg atatatttat
     2461 ccagagagcc tccccgctct tctgcaagac tttcccacga tgtcattgtg gggaccctgt
     2521 aacctgcttt tttgtgcgta tacatccatg ccactctgcc gttatcatct gcggcggcaa
     2581 tgcgctgcca ggcaggataa agcggggtgt atgtttctct gtcattacgg gcgtcaggca
     2641 tggcatcaat actgcaaact tcccccgtaa ggttattcag ccaggccttc gggctaatct
     2701 ctgacgttac gatttcatcc agttttttct ccagattttc tatgtgactg attagcgcaa
     2761 ggataactgg cggagtggca agggagtgaa atgcttccag atcacatccc ttgtcgccat
     2821 atacggtatt ttgtgcggcg tcacgcagtg ctttcagatc agcttctttc atcgttcaga
     2881 ctcccttgtt gctgttttgt aggcgcgcag gatgtcgcgt gtttttcctg ataatgacga
     2941 cttaaccagg aaaaagccgc tccggctttc tgtgatgtcc ggcgtgcaca gaagtaccgc
     3001 atcaacgaca cgattatgtt ttcgcaatgt caggcgggaa ctggtgataa tcagttttgc
     3061 ctgttcgccg taatcggtga atgaaataat catctgatgg cgatctccgt tatgaaagtg
     3121 ttcctgctgg ttcaatgctg ataatttctg ctgcaataag catacctgcc agccatgctt
     3181 cgccgagcat gtcctcatcc gtacatgcaa gatcaccgat ctgtattgtg gccataatgt
     3241 tagtttcacc ggtatattca tcctgtgctt cttcatacgg caggtcttca taaaggtttt
     3301 caattgcgca ggttattaca tccagtcccg tcagattgcc gacaacgtcc acttcaaatg
     3361 tttcgcggta ctcccattgc ccgagtgtca gacgaaccgt ctgtcttgcc atgcgcccgc
     3421 ataatgtcat gttcgggtcg tagttcatga ttgccgcagt agtgctattc atgttctgcc
     3481 tccggtgtgt aaatagcttt gtcgtgttta aattcaccgt tccagttttt cttcataggg
     3541 agttcaccgt tcatgtaaaa ctggtataaa cggtggcatc ctttttccag caatacgggg
     3601 gtgaatttcg tgaatgttgc tttcccgtgt ggagtgatct gtactgcttc ttcggtcagg
     3661 tatctgtcac gggcataaga ggccgtgcgc cagcggggtg ttttctccgg atcgcgttgt
     3721 tcgttgaata cccattttcg gccagacagc caccacatta tctggctggt atttacgccg
     3781 ttcagcgcac ggcagaactg gggaacagtt atgcctttag agaaatgttt ttccagactg
     3841 tctacggttg cagagagcgt tttattttcc agggccatag cttcagcccg ttcttcggcc
     3901 tcaataacca tcaacgcgag ttccttgcga gtgagtacgc gctgaacatc gatgttttcc
     3961 cgctgagtga agtagaactc cacaaggtct tcgtgatagt tccacgcctg atccgtttcc
     4021 agcaattttg cgtggttggc tgcgccgcgt tcggtccaga ggatgaggga tcgaacattg
     4081 ctggcaattt tcacagagta gctttgagat actctgtgct taagttcacg tagttcatcg
     4141 ccttccagca aaaaataatg ctttccaaca acgaatcgtt cttcgtttcg ggaatgattc
     4201 tggcgaatac ggattggtgt tgctccataa cctgccgcca gttgttcagt ggtcacaacg
     4261 cgttgaccgc gatattcgat aatttgcaaa tcacgggctg ccaccggcac aagttcagtt
     4321 gattttttca tcattacacc tcacgaaaag ctgattagtg gaacactctg gtttccggtt
     4381 ctgagttgtc agtggcttca ctgataagcc catccagaat catgtgcatc gctctgtaac
     4441 cttccattgt gatgccggtt ttttcgccgt cgcccagagc gccggtgaat gctttgtaca
     4501 taagaatgct tttgagcagc cctgattccg ctccgtaagt ttctatgagt gcccattcca
     4561 tatagttctg catagcgaga cgtaatggtt tcaggtagat tgtcatgcca ccgttgtggc
     4621 ctctgtacat ggtgcccttg tctgtaccgc cattctcgtt aagcacttcg gtccagccgt
     4681 ttctggtgag ctgttcagag aagaacatgg cggtataaag ccagcgccag tacatgattg
     4741 tcttttccgg cgcgagctca cctgtcatgc ctttttcttc tgcttcactg atgcaacaca
     4801 ctatttccag accatgccag atcggctcgt caaaacagcc ttcatcgagc atttccagtg
     4861 ctttgttgat atcaagcagt ccgtaaggtg tgtggatacc ttcagcggta agaaaaaata
     4921 actgttcagt tgcagtcatg gtttgctctc ttcttaaatg gtctttgcgc gacaagccgg
     4981 caatactgat caatccaggc gttgtatttt tccatccagg catcaaactg ttttctgcgt
     5041 gcaaggatgc gcaacaggcg atgaaaacaa cgcccgtgag ctctgtagta ttcgggcgta
     5101 atttctcccg gatgaaagac ttctccatat tccggatgca tcaggcactg gtcaggcaga
     5161 cgtttttcaa aacctgaact ttcgaatacc ttcgttgtca taaaatgagc gagatgtatg
     5221 atggccgttt ttcgcgtgaa gcatttctta acttgtccgt ggcggcagac tctgtaaaca
     5281 gggccgcaat cgagttcgta tttatgaaaa gcacggtcga ttccggcatt atttattgca
     5341 ctgtattccg catcactgaa gaaaggtgga aatacgatgt atccttccat tgtcagcgcc
     5401 agaacgcgcg gatcggtttc tgtcagccat tcgttttctg tgaattcttc agaattaatt
     5461 tcaatgattt caggttcttc tttcatggtt actccgtgtt catccggtgt taattacttt
     5521 tattcactct gagcgcagcc agaagatgtg gctcagcaat tcgtttgttg cgatatatct
     5581 gtgatacagc ctggcctttt caggttgtaa aaatccccga caataaagct gtaaattaat
     5641 tactgttctt aaataatgtt attcgttttc agtccggcac agatgctttt ctgctttgtc
     5701 tgctgtacag gataatgagc ggaaaagaca attcatcatg tttagcgcat ctggtgaacc
     5761 aatatcaccg ctgtcaataa tcacctgtaa caaagacagg ctttctgtta tctcgcagtg
     5821 gacattatcg agtacttcaa cgcaattatt catttttcat tatctccgtt attccggggg
     5881 aggagctatg ctgttaatga ttaacgcatc tggtaatatg cccctcttta atgtaatcga
     5941 tgcattcatt caaaagttca tcaatgatta actttcctga ttcagtcaga tactcaccat
     6001 gttggttgat accgacagca tcctggtatg cagtgcgtat tgctgtttca ccatctttct
     6061 gcccaaactc accacgggta acgccttcaa atcgtaatag caactggctt atgaactgtt
     6121 ctgttatttc aatagttgtt aactctccat catgacgctt tacagtgagg agattacttc
     6181 ctgtttttcg tttcatccgc cgcagtgctg caaccgttat gcggcgacga tatgcagaaa
     6241 tgtattcatc tttcatttgt tatttcccgt atgccttttt gagaaacaat actgcaactg
     6301 accagtatcc ggcatcagcc attaataaag cggttttata ggcatgtttg tctttcatgt
     6361 gtcatcccca ttttaaaata gtgagaactc ccgaccgcag accgtcatgt ttttatgtat
     6421 gattgtctgt ttaatttcct ttattcgttc tcagagcatc aacatactcg taagcctttt
     6481 cacaggtttt attcatggaa cgaatcatac aaagtaagaa atcatctgtc tctccgttat
     6541 caccagagtt tttaaatata aactcaagca ttgacgtgtt ttcttttatt tctgctgcca
     6601 cttcctcaag cataatcagg ggagttttca tgttctttgt tccttaaatg catcgcatgc
     6661 gcttctggcg tattgttgtg ccagtaaaaa tatgtcatcc gaaagttcat cacattcttc
     6721 atcaccggaa gccgaaatga ttaaccccgc ttcaagcagt actgcaatgt gatgaaaagc
     6781 tgtttccggt tcgttggtga ggcctttgaa cattttcatc ttacgattcc tttgtcgtgt
     6841 cagaatggaa acttgtaagt aaccacatcg gatcgcagtc aagtatattt gccagaggaa
     6901 tcacctggct tgccagaggt tcagccttgc cgttctccca ctggatgatg gtttcttcat
     6961 caactccaag cagcgtggcg agttcagcag tggatagacc gcagctttcg cgttgggtgc
     7021 ggatgccatc acggcaatct tttttgtttc tcggaggtaa cagaatatgt tttagttgtt
     7081 ggattttttc gattccaacc tcaaggagta cgtgacggtt tctttccgat acagactcca
     7141 gactttcatg aagcagcgat tctacttgtt cgataagcat caattgtgaa gtagacataa
     7201 aaaaacctcg taaccaatat ttcgaaatct aggttacgag tgagtaatgt aggtgtcaag
     7261 agcaattaat gttttttgtt gaattaaatt tgcatacgca tctcaacgac aacaccaata
     7321 atttcacaat ccccattgat ggggatcagt ggatagcgtg ggtttagtgg ctttaggaaa
     7381 cattgcccag cgtcttctat gtactgttta agggttgcct ctccggagtt tttgagcctg
     7441 gcgacgacat attttcctga aaaaacttct ttgtcagggt taacaagaat gttcatgcct
     7501 tcaaaaatag agaactctcc attctgtgat gtcattgagt cccctttcac ttccagccag
     7561 aagccgtgat cgcccgcata tatatcgcta ccaatccatt cttctggata agctatgtaa
     7621 tcttcgcagt tccaggggcc agcactcacc catgttagtt tcggatatct ataacggttt
     7681 ggagaaactg gcttaggatt tcggacgttt tgaatgctat ctgtaggtga tggggcgctg
     7741 ccgtcaagaa gccatttagg agagcatttc aatgcatccg cgattctaaa taggtttgaa
     7801 gatcttgtgt cttgtgagtc tcctaactct attttgctga ttgttacacg cgagacaccg
     7861 gtcaattttg ccaggtcatc ttgtgacatt cctagctgta ctctgcgcat taacagtctg
     7921 ccaccaaagc tatcgtgatc catcttctcc cccttcttgt cgaaataatc gtttcgaaac
     7981 aaatatagca aaaaatcttg aaatctaggt ttcggaaaat taagattaat cgaaacaaac
     8041 gtgtcgaggt ggatatggat ttgtatgaaa ttctaaaaaa catgtttgga tcgaatgttg
     8101 aaataggtcg ttattttccc cgaaggggaa gggctagaac cggacaggct gtaggtaagt
     8161 ggaaaacccg aggtgtgccg gaagacgtgg cgatcctttg tcatctggat cccaaaattc
     8221 cttatcagca tccgtcgctc atgaatgcca gtcatgaatc gtgatgttct gccggaggct
     8281 gtatgtcccc tgattacgtt cagatggaga tgcctgccag gtacagccag gcagattcag
     8341 agtggattaa gcggcagtta cagagcctgc cttcctcact gagacagaag gtcgccctga
     8401 aatacgcgga ggtttacgaa gttacttttg attccgagcc tgtttcattc cgtaaggaga
     8461 acagagcgag gcatgaagcc aatgtacggc tgcgcagatt cgttgaaaca cacgggcgtg
     8521 cattacaggg gtatacgact cagccgcccc tggcaggaac gcaacagcgt acctgaatgg
     8581 tgttggtctt aaaggtgacc gacgggctga gaccccactt ctcaggatgc acttatgtac
     8641 taccaagcta gtacata
//
LOCUS       DAZUCC010000199         8619 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZUCC010000199 DAZUCC010000000
VERSION     DAZUCC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.200"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..686)
                     /locus_tag="ACHY09_004123"
     CDS             complement(<1..686)
                     /locus_tag="ACHY09_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFS3887018.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPAHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKR"
     gene            complement(697..1698)
                     /gene="ltaE"
                     /locus_tag="ACHY09_004124"
     CDS             complement(697..1698)
                     /gene="ltaE"
                     /locus_tag="ACHY09_004124"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFS3887019.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1735..3453)
                     /gene="poxB"
                     /locus_tag="ACHY09_004125"
     CDS             complement(1735..3453)
                     /gene="poxB"
                     /locus_tag="ACHY09_004125"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFS3887020.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3586..4554)
                     /gene="hcr"
                     /locus_tag="ACHY09_004126"
     CDS             complement(3586..4554)
                     /gene="hcr"
                     /locus_tag="ACHY09_004126"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFS3887021.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4566..6218)
                     /gene="hcp"
                     /locus_tag="ACHY09_004127"
                     /gene_synonym="priS"
     CDS             complement(4566..6218)
                     /gene="hcp"
                     /locus_tag="ACHY09_004127"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFS3887022.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6362..7261)
                     /gene="lysO"
                     /locus_tag="ACHY09_004128"
     CDS             complement(6362..7261)
                     /gene="lysO"
                     /locus_tag="ACHY09_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFS3887023.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7718..8413)
                     /gene="aqpZ"
                     /locus_tag="ACHY09_004129"
     CDS             complement(7718..8413)
                     /gene="aqpZ"
                     /locus_tag="ACHY09_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFS3887024.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAAAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
BASE COUNT         2144 a         2368 c         2223 g         1884 t
ORIGIN      
        1 cgcttaccgc tcaccgccat aaaatgttca aactgttgct gataactgag cacttctggt
       61 ccggcggctt cgaagatgcg atgttcgctg gccggatgat ctaacagcgc caccagatag
      121 tgcagcaagt tttccagcgc gatgggcgtg gtgcgtgaac gaacccagcg tggcggcgtt
      181 aacaccggca ggttgtagac catatcgcgc atgacttcga acgccgctga acctgcacca
      241 acgattattc cggcacgtag ttcggtcacc gggacacccg cttcacgaag aatatccgcc
      301 gtagcctgac gggcgcgcag atggtcagac tgctcatgtg ccggggcctg caacgaactg
      361 aggaagatta attgcttaac agggacttcg cgtagcgcat cgcggacgtt gagagccacc
      421 tggcgctcct gagcgataaa atcgccgcct tcgcccatgc tgtgcaccag aaaatagacc
      481 gtatcgatat cctgcaacag ggccggaagg ttatccggcc agctgagatc gactttatgg
      541 caactgacgt ttgccagttg cagctttgca agcctgtcga catgacgtgc cgccgccagg
      601 atctgatgcc cttgctggct gagagttcgc accagatgct gaccaatgta gccactggca
      661 ccgagaacta aaatgcgttg cggcacgtct ctctccttaa cgcgccagga acgcacgcca
      721 gtgggcggca acttccgcca gttgttcacg cgagacgtca agatgggtca ccaggcggac
      781 aatcggcgag gcgttaatca gcacgtttct cgctttcatg tattcgccta acgcggcagc
      841 attttcttcc ccgacgcgaa caaacagcat attggtgtcc tgacgcatca catccgcgcc
      901 tgcttcacgc aactgctccg ccatccaggc ggcgttatcg tggtcttcct gcaaacgtgc
      961 gacgttattt ttcagggcat acatcccggc ggcagccaga atgccggact gacgcatccc
     1021 gccacctgtc attttccgcc agcgaatggc acgtttaatg taatcacgat taccgacgag
     1081 taatgaaccg accggcgtcc caagaccttt cgacaggcaa atggtgaacg aatcacaata
     1141 ttgcgtgatc tctttcagtt cgcagccgta agccaccacg gcattaaaga tgcgcgcacc
     1201 gtcaacatgc agcgccagat tgcgctcgcg agtaaattcc catgcttctt tcaggtattc
     1261 ccgcggcaac actttgccgt tgtgggtgtt ttccagactg agtaatttgg tgcgggcgaa
     1321 atggatatcg tcgggtttga tcttcatcgc cactttatcc agaggtagcg tgccgtcggc
     1381 agccgcgtcg atgggttgcg gctgaatact gcccagcact gctgcgccac cggcttcaaa
     1441 cagatagtta tgcgcggcct gaccgacgat atactcttcg ccgcgttcgc agtgactgag
     1501 cagagcgacc aggttggcct gagtgccggt aggcagaaaa atggcggctt ctttaccgga
     1561 aagctctgct gcatagtcct gcagagcatt aacggtaggg tcgtctccgt aaacgtcgtc
     1621 cccaaccggg gcggccatca tcgcttcgag catggcgcgg ctcggtcggg taacggtatc
     1681 actgcgtaaa tcaatcatgg catgtcctta ttatgacggg aaatgccacc ctttttacct
     1741 tagccagttc gttttcgcca gctcgatcac ttcatcgccg cgtccgctga taattgcgcg
     1801 cagcatatac aggctgaaac ctttggcctg ttcgagtttg atctgcggtg gaatggctaa
     1861 ctcttctttg gcgaccacca catccaccaa caccggaccg tcgatggaga aggcgcgttg
     1921 cagggcttca tcgacttcag aggctttttc tacacgaata cccgtaatgc cgcacgcttc
     1981 ggcaatgcgg gcaaagttgg tgtcgtgcag ctcagtaccg tctgtcaggt agcctccggc
     2041 tttcatctcc atcgccacaa agcccagcac gctgttatta aagacgataa ttttcaccgg
     2101 cagtttcatc tgcactactg agaggaaatc gcccatcaac atgctaaaac cgccatcgcc
     2161 gcacatggcg accacctgac gttccggctc ggttgcctgc gcacccagcg cctgcggcat
     2221 ggcgttagcc atcgaaccgt ggttaaacga acctaacagg cgacgcttgc cgttcatttt
     2281 tagataacgt gccgcccaca ccgttggcgt accaacgtca caggtgaaaa tagcgtcatc
     2341 ggcggcaaaa tgactaattt gctgcgccag atattgcggg tgaatggctt tctcgctcgg
     2401 tttagctaaa tcgtccagcc ctttgcgggc gtcgcggtaa tcttccagcg ctttatccag
     2461 aaacttgcga tcggcttttt cttccaccaa tggcagcaat gcacgcagag tcgacttgat
     2521 atcgccgacc agtgccatat ccaccttgct gtgagcgccg atgctggctg ggttgatatc
     2581 aatctgaata attttggcat cggtcgggta gaacgcacgg tagggaaact gagtaccgag
     2641 cagcactaac gtgtcggcgt tcatcatggt atggaaacct gacgaaaagc cgattaatcc
     2701 cgtcattcca acatcatatg gattatcgta ttcgacatgt tctttaccgc gcagggcatg
     2761 gactataggc gctttaattt tcccggcaaa ctcaactaac tctttatgcg cccccgcaca
     2821 gccgctgcca cacatcaggg cgatattgct ggaataacgc agcagctgcg ccagtttgcg
     2881 taactcttct tcttccggtg ttactaccgg ctgtggcgca tgataccagt gggtagttgc
     2941 cccttctggc gcaggtttta acgccacgtc gcccggtaac acaacaacgg aaacgccacg
     3001 gttaagcacc gctttgcgca tagcaattgc cagcacttgt gggatctgct ctgggctgga
     3061 aaccagctcg caatagtgac tacattcgcg gaatagctct tgtgggtggg tttcctggaa
     3121 atagccgctg ccaatttcgc tggagggaat atgagcggca atcgccagta ccggaacgtg
     3181 attgcggtgg caatcgaaca ggccgttgat taagtgcagg ttgccgggac cgcacgatcc
     3241 ggcacagacc gccagttctc cgctaagttg tgcttcagcg ccagcggcaa atgccgccac
     3301 ttcttcatgc cgggtggaca tccactcgat ggtgcccatg cgattaagac tgtcactaag
     3361 accgttcaga gagtcgcccg tgactcccca gatgcgtttc acgcctgccg attcgagtgt
     3421 tttggcgata taagctgcaa ccgtttgttt catggttctc catctcctga atgtgataac
     3481 ggtaacaagt ttagttcatc tgacggaggg ggaagggatg ggagaaaaag gaggcactaa
     3541 cggttaaata gcccgatgaa aggaatatca tcgggcatca ggcgattatg cgagaaccaa
     3601 atccccctgc ggatggcagg agcaggccag tacataacct tcagcgattt cggcgtcggt
     3661 cagcgtcatt gtgctgctca ccgtatattc accggaaacc actttcgtct tacagcagcc
     3721 gcaaacacct gcgcggcagg cggcgacaac cggaacgtta ttgctttcca gcgcctccag
     3781 tagcgtggtg ccaaccgggg cgtaaaattc tcgtgccggt tgcagtttgg tgaatttcag
     3841 accgctggtc gccgcttccg ctactggggt gaagaatttc tctttaaaga aacgcgtcac
     3901 gccgagcgct ttcacttcct gctctaccca atccatatat ggagcagggc cacaggtcat
     3961 cacggtacgt gaggctaaat cagggacacc tgccagcagt tcgcgagtga gacgaccagc
     4021 gataaagcct tcggtaacgt tattttccgc caccagcgtt accggatagt tacgccactc
     4081 atcggcgaaa atcacatcct gcggcgtacg cacgttgtag atcacctgca catcggcctg
     4141 tggacggttc ttcgccagcc agcgacgcat cgacataatc ggcgtaacgc cgcagcctgc
     4201 cgccagcaac aggaatttat cttctgcttt atcatcgcag gtaaattccc ccatcgcgtc
     4261 cgaaagccag agataatcac cgcgttttac atcgcgcgtc agccactggg agccgacacc
     4321 gtcatcaatc cgccgcacag tcagggtgat gtattcactc acgcctggcg tggaggaaat
     4381 ggtgtaagca cgcagcgttt ccgctgagtt acgcacgctg accagtgcat attgcccggc
     4441 gcgatatggg tagtaatcgt ggcaaatcag ggaaatcgtc cacacatccg gcgtttcttg
     4501 cgtaatgtga tgaacctgca tccgccacgg gcattgattc gttggcatcg tcatcgacaa
     4561 actccttacg cgctcaacag ttgcttcatg tcttcttcaa cagtggtgat agaacgcagg
     4621 ccgaatttct cgttcagcac cgccagcagg tctggtgtca ggaaaccagg tgcagtcggg
     4681 ccggtgacga tatttttcac acccagagaa agcagcgtca gcagaatgac aatcgctttc
     4741 tgttcaaacc aggagagcac cagcgacagc ggcagatcgt tgactccgca gcccagtttc
     4801 tctgccagcg ttaccgccag aataattgct gagtaagcat cgttacattg accagcatct
     4861 accagacgcg gcagaccttc gatatcgcca aactcaagtt tattaaagcg atatttaccg
     4921 caagcgaggg tcaggatcag gcagtcatcc ggcacgctgg tggcgaaatc ggtgaaatag
     4981 tgacgctcgc cgcgtgcgcc gtcacagccg ccaagcagga agatatgacg cagtttttca
     5041 cggctcacca gatcaatcag cgtatcggct gcgccaagca gtgtctggcg accaaagccc
     5101 acggtgatca ggtgcggaat ttcgctgtac gggaagcccg ccatctgttg cgcctgggca
     5161 atgactgcag agaaatcttc gccatccaga tgacgcacac ccggccagcc aacgatgctg
     5221 cgggtccaga tacgatcgtc ataagcgcct acggttgggt cgatgatgca gttcgaggtc
     5281 atcacgatgg ggcctgggaa acgagcgaac tccacttgct gattctgcca gccgctgccg
     5341 tagttaccga ccagatgctt gaatttacgc aactccgggt agccatgcgc aggcagcatt
     5401 tcgccgtggg tgtaaacatt aacgcccgtg ccttcggtct gttccagcag gttgtagaga
     5461 tctttgagat cgtgaccgga aatcagaatg catttacccg ccgtcgcttt gacgttgacc
     5521 tgagttggcg tcgggtgacc gtatttaccg gtttcgcctg cgtccagaat gctcatcact
     5581 ttgaagttca tctggccgat ttccattgaa cactcaagaa gcgcgttcat atcggcaggc
     5641 caggtcccca gccacgccat gattttatgg tactgggcat aaatatcgtt gtcgtattga
     5701 ccgagaacat gcgcgtgttc catataggcc gccgcacctt tcaggccata caggcacagc
     5761 agacgcaggc cgagaatgtt ttcgccaatc gccgctttat ctttgttagg ggtaaattct
     5821 gctgcctgac gttgcagctc gccgagatcg tcgctcacca gttgcaggtc agccatcggg
     5881 ttatcgacgc gggcgttggc atctacagcc aggcattgtg ctttcagcgc ctcgcgcagg
     5941 gcaatcgctt cacgagcgta gccgacaata cgcggagaat cgaagttaac gttggtcagg
     6001 gttgagaaaa aggcacgtgg cgcaaagctg tctacatcgt ggttgataat gccgtattca
     6061 cgtgctttca ctgcccaggc cgaaagccct tgcagcgccg cgatgagtaa atcctgaagg
     6121 tcagaggttt ctgccgtttt accacacatc ccctgcgcgt atgagcagcc gtttcctgcc
     6181 ggagtacgga tagtttgttc acattgcaca caaaacatga tcacaccttt taaagttata
     6241 tttaatatac atgtttaagg ttaagacgct taacgcgggg ataaaagaga tttttaatgc
     6301 aactttaagg gagattgatt tagcgcaatt ttggcggcag agatctaccg ccagagaggt
     6361 attatgcaga gaaaaaggcg atgagaatcg gcactaacag actaagaata aaaccgtgaa
     6421 caatcgccgc cgggaccata tccagcccgc ctgtacgttg aagaacgggc agggtgaaat
     6481 ccatcgacgt cgcaccgcat aagcccagtg cagtagagcg gctacggcga atcagcccag
     6541 ggattaacat aatagcaatc agctcacggg ccagatcatt aaaaaacgcc gcgctcccga
     6601 ttaccggacc aaaagattcg gtcaataaaa taccggaaag agaataccag ccgaaaccgg
     6661 aggccattgc cagcgcggta ttgatgggaa gatcaagaat aaaggcgtta attaaaccac
     6721 caattaatga actggcaacc accaccacgg cgacaatcat tccccggcga ttaaggacaa
     6781 tctgctttaa ggtcatgcca ttattgcgca actgaatacc gacgaggaaa agtagcagaa
     6841 ttaaggtgta ttcactggct tcggtcgcgt gttgtaagaa agccagtcca ctcagaccaa
     6901 tggcaaaacc aatcactact acgccgcaca gttttagcga ctccagcgcc atcgcaatac
     6961 gcgacgggag tttttcttga tgatggtggt tgcgccatgg caggccgcgc tccagccaca
     7021 tcagggcggc aatattacac agtaaaataa cggtaatact gacggcagaa taatgcagaa
     7081 tcgccaacag gttactggcg aggttatcga gaaacgccag actgataccc ataaaaaaga
     7141 gaataaggta aaccatccag cttaatagct gattaataac tcttaatgca gctttttggc
     7201 gaagcggaat gaggtaaccc acaatcaggg gaaccagaat gattaacagc ccagaaaaca
     7261 tgaaaaccca gtccttgcaa agatgaagtc gaaatgcgcg atgacacact actgaaagcg
     7321 gaaggacgag taaagttgca attaaaagga aatgttatgc atcagaggga gatttttaat
     7381 tctatatgag tgtttagcat tgttgcgctc ctttcatgtg caatgccata taaagccact
     7441 ggcgcaggga gcgcgcaggg ggcggccagt cgccgccgcc ccctgcaccc ccgggctctg
     7501 gcgaacaaaa tcgccgctgc gcggtgcctt cagcttatcc ccttcggcta ccgggtcggg
     7561 cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat ccatgtgggc tgccccggcc
     7621 ttcggggaac gcttcagcga ttttgacgcc accaacaccc gtgcggtttg taacgtcgaa
     7681 gataaaacaa ccagggcaga taaaatgcca atgctaatta attacgcttt tccagcaggg
     7741 tccggtaaat cagaccaccg ataataccgc cgacaattgg caccacccag aagaaccaca
     7801 gttgttctaa cgcccagccg ccctggaaga tagcagccgc ggtgctgcgc gccgggttaa
     7861 cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc agaccaatag
     7921 cgatcggcgc aaaaccagca ggagcgaatt tgtcggttgc gccgtggatc accagcagga
     7981 aacctgcact caataccagt tcaactacca gcgcggaaag catggaataa ccgcctggcg
     8041 aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa cctgttttac
     8101 cactggcaat taaatacagc agcgccgccg caacaatacc gccgacaacc tgggcaatta
     8161 cgtagccaac gacttctttt gccgggaaac gtccgccagc ccataaacca atagtgaccg
     8221 ccgggttaaa atgaccacca gaaatatgac caacagcaaa ggccatcgtc agaacggtca
     8281 gaccgaatgc cagcgccacg ccggcaaaac caatgcctaa ttccgggaag cctgcggcca
     8341 gtacggcact accacagcca ccaaaaacaa gccagaaagt accaaaacat tcagctgcta
     8401 attttctgaa catatccacc tcaattaaaa attgaccctg tgaaaaatat ggtcgtttta
     8461 tagggtcgtc gtaaaaaagt gacgacggaa ataatgcgcg gctattttaa aaacgaaggc
     8521 gagtcattca ccagataaat aaatccagta aatttgattt aaggcaacag cgggttgccc
     8581 catatagtca tttgtctgat tgacagtgta gtgcaagcg
//
LOCUS       DAZUCC010000200         8555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZUCC010000200 DAZUCC010000000
VERSION     DAZUCC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.201"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..295)
                     /locus_tag="ACHY09_004130"
     CDS             complement(104..295)
                     /locus_tag="ACHY09_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFS3887025.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            313..465
                     /locus_tag="ACHY09_004131"
     CDS             313..465
                     /locus_tag="ACHY09_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887026.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     YVIMMS"
     gene            647..2890
                     /gene="pdeF"
                     /locus_tag="ACHY09_004132"
     CDS             647..2890
                     /gene="pdeF"
                     /locus_tag="ACHY09_004132"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFS3887027.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEISQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2929..4470)
                     /gene="ppx"
                     /locus_tag="ACHY09_004133"
     CDS             complement(2929..4470)
                     /gene="ppx"
                     /locus_tag="ACHY09_004133"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFS3887028.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4475..6541)
                     /gene="ppk1"
                     /locus_tag="ACHY09_004134"
     CDS             complement(4475..6541)
                     /gene="ppk1"
                     /locus_tag="ACHY09_004134"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFS3887029.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6712..7350)
                     /gene="purN"
                     /locus_tag="ACHY09_004135"
     CDS             complement(6712..7350)
                     /gene="purN"
                     /locus_tag="ACHY09_004135"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS3887030.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7350..8387)
                     /gene="purM"
                     /locus_tag="ACHY09_004136"
     CDS             complement(7350..8387)
                     /gene="purM"
                     /locus_tag="ACHY09_004136"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS3887031.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
BASE COUNT         2121 a         2095 c         2091 g         2248 t
ORIGIN      
        1 aaatttaaga tgaaaaaata tctgccgctt tgataatccc aaagagcaaa agcatccgac
       61 agcgaggtgc cgttatgttc tgtgagagtg gagaaggtaa ccgctaacgt tgtactggtg
      121 attcgacgga gagtgtggat tgctgagctt cttttttgct ctgatggtgc tgccaggcac
      181 cgacggacga gtagataaaa cggccaaaga agaagataaa gctgatgagc agtacgatac
      241 gggtcatgcg actgttaaat cggtgtcgtt ttcgcatact ggttgcctga ctcacaaaag
      301 gttccttgaa gtatgtccca cgccgtggac ggtacttaca ttaaggcaca acaggacaaa
      361 atggtcaatt cttcgttatg taaaaaagcg tcagtggata catattttaa tgttatggaa
      421 gttaatttaa ttatttacaa tgattatgta ataatgatga gttaacatga taaacgtaat
      481 aattcattaa tcagggttat tttgtttgat atatatcaat tggaactttc atatgacact
      541 tagaatcatt gctctctata gtgatgaata atcatcattc gaagtcaggt gggatgtctg
      601 tctgaataca cctccttcag gatgtggggg attctgctga gcatctatga aactgaatgc
      661 aacttatata aaaatacgtg ataaatggtg ggggcttccg ctgttcctgc cttctttaat
      721 cttgcccatt ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg aagtttttct
      781 cttttatctg ccactggcac tgatgatcag catgatgatg tttttcagct gggcggcatt
      841 gccagggatc gccttaggga tttttgtccg caaatatgca gagctgggtt tttacgaaac
      901 gctctcatta acggctaatt ttattatcat tatcattctc tgttggggcg gttacagggt
      961 ttttactccc cggcgtaaca acgtttcaca tggtgatagc cgtttaattt cccagcgact
     1021 attctggcag attgtgtttc ctgcaacgct gtttctgata cttttccagt ttgctgcgtt
     1081 tgttggatta ctggcgagca gagaaaatct ggtcggcgtc atgcccttta acctcgggac
     1141 cttaatcaat tatcaggcct tgctggtggg taatctgatt ggtgtcccgc tgtgctactt
     1201 catcattcgg gtggtgcgaa atccgtttta tttacgtagc tattattcgc aattaaaaca
     1261 gcaggttgat gccaaagtta ccaaaaaaga gttcgcaatc tggctactgg cattaggtgc
     1321 tttactattg ctgttatgca tgccgttaaa tgaaaaaagc acgattttta gcaccaatta
     1381 caccttgtca ttattgctgc ccctgatgat gtggggagcg atgcgctatg gttataagct
     1441 gatttcattg ctctgggcgg tcgtgttgat gatcagcatc cacagctatc aaaattacat
     1501 tcccatttat cctggctata ccacgcagct gaccataacc tcctccagtt atctggtatt
     1561 ctcttttatt gtcaattata tggctgtact ggcaacccgt cagcgagcgg tagtcagacg
     1621 cattcagcgg cttgcgtatg tggacccggt ggttcatctg ccaaatgttc gcgccctgaa
     1681 tcgcgcgtta cgtgatgccc cctggtctgc gctttgttat ttacgcatcc ctggcatgga
     1741 aatgctggtt aagaattatg gcatcatgct gcggattcaa tacaagcaaa aactttctca
     1801 ctggctgtca ccattgctgg aaccgggtga agatgtttat cagctttcgg gtaacgatct
     1861 cgcgctgcgg ctgaatacag aatcgcacca ggagcgcatt accgcactgg atagccatct
     1921 caagcaattt cgtttctttt gggatggaat gccgatgcaa ccgcagattg gcgtcagtta
     1981 ctgctatgtg cgctcgccag tgaatcatat ctacctgctg ctgggagagc taaatacagt
     2041 ggccgaactt tctatcgtga ccaacgcccc ggaaaatatg cagcgtcgcg gagcaatgta
     2101 tttgcaacgc gaattgaaag ataaagtcgc gatgatgaat cggctacagc gggcgctgga
     2161 acacaaccat tttttcctga tggcccagcc gattaccggt atgcgtggtg atgtctacca
     2221 tgaaattctt ctgcgcatga aaggtgagaa tgatgaactg atcagccccg atagcttctt
     2281 accggtcgcg cacgaatttg gtttatcgtc gagtatcgac atgtgggtca ttgagcatac
     2341 gctgcaattt atggctgaaa acagagcgaa gatgcccgct caccgttttg cgattaatct
     2401 gtcgccaacc tcggtatgtc aggctcgttt tcctgttgaa atcagtcagc tgctggctaa
     2461 atatcagatt gaagcgtggc aacttatttt tgaagtcacc gaaagtaatg ctctgaccaa
     2521 tgttaagcag gcgcaaatca ccttgcagca tcttcaggaa ttaggctgcc agattgcgat
     2581 tgatgatttc ggcaccggct acgccagcta tgcgcggctt aaaaatgtga atgccgatct
     2641 gcttaaaatt gacggcagtt ttatccgcaa tattgtgtca aatagtctgg attatcagat
     2701 agtggcgtcg atttgccacc tggcgcgaat gaagaaaatg cgggtagtgg cagagtacgt
     2761 tgaaaacgaa gagatccgcg aggcggtgct ctctttgggg atcgattata tgcagggtta
     2821 tcttattggt aagccgcaac cgttaattga tacgctgaat gaaatcgaac ccattcgcga
     2881 aagtgcctga ataatgcggg ccgacatttc tcgtcggccc gcaaagcatt aagcggcgat
     2941 ttctggtgta ctttcttctt caattttcaa ccgccagcca gccacgcctt cccagtattc
     3001 ttgctccttt tccagatcaa gcagtaccag cgcattctga ctaaaccagt catgcggaaa
     3061 acgcagtgtc cagtgactgt catcggtaat cagcgtcaat gttggcggtg tggtggttgc
     3121 ctgacgttga ttattgagta atacgccaag gcgcaatagc tgaatcagtg gcaggaactg
     3181 tttcttctta aacaaggtaa agcgcggcag atcgtcgagc ttaatcgctt tacggtgata
     3241 gcgcaccagc gtcgccatca tcaactgctg ttcctgatta aaacccggca agtcgctgtt
     3301 ttgcagaata taagcggagt ggcgatgcaa accgctgtgg ttgatattca acccgacctc
     3361 atgcagcatg gcggcccatc gcagtagcgc ttccagttgc ggatgcgcca gcttcggttg
     3421 ctgttcccgc cactgttcgt acatttgcat agtggtatcc agcacccgtc gggcctgttc
     3481 gctgtcgatg tgatactggt tggcgaggct gctggcggtg cgactacgca catcctgatg
     3541 acggaaacgt ccttccattt catacagtac gccttcgcgt aacgccccgt cagaaaggcg
     3601 cagttcacgg atggctaaag catcaaacac accgcataaa atcgccagtc caggaacgaa
     3661 gactgttttc cgttcttcgg aaagacctgg taagctcagc gatgcgaaat tacggtgccg
     3721 taaaacttct tttaccagtt tttccagacg ttccggggta attatcccgt ctttctcgcc
     3781 catttccatt aacacttcat gggcggcttt tattgtaccg gaagcgccca ttgcaacatt
     3841 ccagccctga attcgaaatt gccaggttaa agtttccagt ttttgtgctg ccgccatgcg
     3901 agcgcgctga aaattctctt tattgatgac cccgccaggg aaataaagct gggcaaagct
     3961 gacacaaccc atccggcggc tttcaacgag aatcggttcg aaattttcac caatcaccag
     4021 ttccgtagat ccgccgccaa tatcaataac cagtttgcga cctttctccg gttgggtatg
     4081 ttccacgccc ataaaaatca gacgggcttc ttcattaccg gaaataattt caatcgggta
     4141 gggaatgacc ttttccgcgc gtttcagaaa gtcagtggcg ttcagcgcct gacgcagtgt
     4201 atgggtgcca actatacaga cgctggcagg agaaaaccct tgcagccgtt cggcaaacag
     4261 cgacagacag tttaaaccgc gcgtcattgc ctcttcactc aacatattat ctggccccag
     4321 gccgtccgcc agatgcactc gctgtttcag gcggccaata atctgcatgg caccatctac
     4381 cacacgggct atgaccatgt gaaaactgtt tgaaccaaga tcgaccgcag caaactcctg
     4441 cggacgaggg gatttatcgt gtattggcat agggttattc aggttgttcg agtgatttga
     4501 tgtagtcgta aatcgccaac tgcgcccgta ctttacggcg attgccgcgg ggaacgtagc
     4561 gattactgag ttctttatcg atataacgtg ctttgaccgt atcgctgaac aatatgtcga
     4621 tgatgtccag cacccgctgc ttcaggcgcg gatcgagcag cggtgtcgcc acttcaatac
     4681 gataatcaat attgcgcgtc atccagtcgg cggaagaaag gtagaccttt ttatcgccgc
     4741 cattttcaaa aatataaacc cggtcatgtt caaggtaacg gtcaacaata ctgatggcac
     4801 gaatgttgtc gctaatgcct tccagattgg ggatcagcga acacatgccg cgaaccagca
     4861 gattaaccgg tacgccggag ctggaggccg catacagacg atcaaccagg cctttatcga
     4921 caaggttatt tagcttcagg gtgataccgc tgggcagccc ttgctgcgcg ttggcgattt
     4981 cgcggtccac catttcatac aacaggcggc gggagttttg cggcgacacc attaaataat
     5041 caaatgtcac cggacggtat gggttttcaa taaagttgaa cacccgccgt acttcgttgg
     5101 ttatgcgcgc atcggcggtc agcaacgaat agtcagtata aagacgcgcg gttttttcgt
     5161 taaagttccc ggtcccgatg tgtgcgtaac gcaccacttc accgttttct ttacgtgaaa
     5221 tcaggaacag tttggcgtga attttcagcc ccggcgcaga gaagataacg tgcacgcctg
     5281 cttcggtcag gcgcttcgcc cagtgaatgt tggcttcttc gtcgaaacgc gcctgtaact
     5341 caaccaccac ggtgactttc ttaccgttgt gtgcggcgtg gatcatcgag tcgatgatgc
     5401 gtgaatcttt cgccacgcgg taaatgttaa ttttaatcgc cagtacgctc gggtcgaacg
     5461 aagcctgacg cagcagttcc agtacatgct caaaggtatg ataaggataa tagagcaaca
     5521 catcgcgttc gcgaatagca tcaaaaccat tgcggaactg ggctttatca aaccaaatgt
     5581 ggcgtaaacg cggcagtggt ttgttcacca gattggcttt gccgacattg gggaaattaa
     5641 taaagtcttt aaaattatga taacgaccac cggggacgat ggagtcgtag cgggaaatag
     5701 tcagtttttc gcgtaacact tcaaccagcg cattgggcat atcgcgctga taaacaaaac
     5761 gcaccggctc agcagttaaa cgctgcttga gactggaaga catcaactcc atcaggctgg
     5821 cttccatctc atgcactaaa tcgtattcgg catcgcgggt catcttcatt gaataggcat
     5881 tcagcgcgtc ataatcaaag aagcctttga aaatatcatc gaggcagtaa cgcagaatgt
     5941 tatccagaag aatcatcggc ttgcgtcgac gtggcgcttc tggcggtaaa ttcacaaagc
     6001 gcggcacttt atctgatggg atctccagca gcgcgtaacg gatggtatcg ccacggataa
     6061 tttccaccgc cagataggtg taatcatctt tcaggaactg cactaagtca gtgtcaggat
     6121 tgattaaaat cggcgtaatg tgctgacgca gatactgctt aaaataatga cgcagccagt
     6181 tttgttgatt gacggagagc tggcgttcat taatcaggaa gatctggttg cgcgccatct
     6241 ccagcaacag ctcgttgtag aggccgtcga attcctgatc ggctttcagc acccgggact
     6301 gaattttgcc cagtaaatgg cgggaatgag agttggaacc ttgttcttcg ctaataatga
     6361 tgcgtcgctt cagttcagcg aagcggactt tatagaactc atcaaggtta ttggaataga
     6421 tccccaggaa acgcatcctt tcaatcagcg ggttagattt gtccgccgct tcctgaagca
     6481 cgcgttcatt gaacgataac caactgagct ctttttcgat gtatagcttt tcctgaccca
     6541 ttaccacttt tactccgttt tattcacggg acactgcctg gatattatgg cgagcaatat
     6601 tggcagatgt ccaactatgc cagaaaaggg ggaaaaaaac tgctggagct tgcgatttac
     6661 aggtgaattg aaaaacgcca gcggcagagc tggcgcttta actacggggc attactcgtc
     6721 ggcagcgtag ccctgcggcg gcagacgttg accatccagc cacgcggcgt tttcgtgcat
     6781 tttcagacga ccatcggcaa accagctaat caccagtgga taaatagcgt gttcctgggt
     6841 ttgcacgcgg gcggtgatgt catcttccgt atcaccagca aataccggga ctttcgcctg
     6901 taaaataacc gggccaccgt ccagttcatc ggtgacgaaa tgcaccgatg taccgtgctc
     6961 ttcatcgcca ttttccagcg cctgacgatg ggtgtgtaat ccgggatatt tcggcagcag
     7021 agaagggtga atgttcagca aacgcccggc atagtgggag acaaacgccg ggctgagaat
     7081 gcgcataaaa ccagccagca cgaccacatc gggtgcgtac atgtcgattt catgaatcaa
     7141 ctcccggtca taggcttcac gactgtcaaa cgcgctggcg atgagcgtat gcgttgcaat
     7201 acccgcctgg cgggcgcgtt caaggccgaa cgcgtcggcc ttattgctga aaactgcccg
     7261 tacggtgcct ttaattttgt tggttttgca ggcgtcaata attgcctgca aattacttcc
     7321 gttgccggaa ataagcacca caatattcat tattcgataa ccacgcgttg ttcggaatca
     7381 gaggctttga tgataccgat tttccacgcg ttttcaccgt tggcattgag cagggcgaga
     7441 gctttgtcca cttccggagc aggcagggca ataatcatac cgacgccgca gttgaaggtg
     7501 cgatacattt catggcgctc aacattacct gccgtttgca gccagttgaa cacttccggc
     7561 cactgccagg aagattcatc aatcactgcc tgggtattat ctggcaatac gcgcggaatg
     7621 ttttcccaga agccgccgcc ggtcaggtgc gcaatggcat gcacatcgac cttttcaatc
     7681 aatttcagca ctgacttcac gtaaatgcgg gtcggtgcca gcagatgatc ggctaatggc
     7741 ttaccatcaa gttcggtggt ttgcggatca caaccgctga cttcaagaat tttgcgcacc
     7801 agcgaatagc cattcgagtg cgggccgctg gagccgagtg caatcagcac atcgccgtcg
     7861 ctgactttag atccgtcgat gatttctgat ttttctacca caccaacgca gaaaccagcg
     7921 acatcgtaat cttcaccgtg atacatcccc ggcatttccg ccgtttcgcc acccaccagt
     7981 gaacagcccg attgcagaca accttccgca atgccgctga tcaccgctga agcggtatca
     8041 acatccagtt ttccggttgc gtaatagtcg aggaaaaaca gcggttccgc accttgcacc
     8101 accaggtcat taacgcacat ggcgaccaga tcaataccaa tggtgtcgtg acgttttaag
     8161 tccatcgcca gacgcagctt ggtacctacg ccgtcagtgc cggaaaccag cacaggttca
     8221 cgatattttt gcggcaatgc acacagcgca ccgaagccgc ccagaccgcc catcacttcc
     8281 ggacgacgcg ttttcttcac tacgcctttg attcttccaa ccagagcatt acccgcgtca
     8341 atatcaacac cggcatcttt gtagctaaga gaggttttat cggtcactgc ttgggtcccc
     8401 acgcgttact tgcggtagaa aaataaaatt cggcgcaatt ctaacaggga aagcaaacgt
     8461 ttgcgagact gctttacaca acctttttgc acgtcttttc cccaggcgcg cggcgaaaga
     8521 atacttgtac tagggcaaag gatcgttttc ggatg
//
LOCUS       DAZUCC010000201         8463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZUCC010000201 DAZUCC010000000
VERSION     DAZUCC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.202"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            414..1391
                     /gene="glaH"
                     /locus_tag="ACHY09_004137"
     CDS             414..1391
                     /gene="glaH"
                     /locus_tag="ACHY09_004137"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFS3887032.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            1411..2679
                     /gene="lhgO"
                     /locus_tag="ACHY09_004138"
     CDS             1411..2679
                     /gene="lhgO"
                     /locus_tag="ACHY09_004138"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFS3887033.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALQEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2702..4150
                     /gene="gabD"
                     /locus_tag="ACHY09_004139"
     CDS             2702..4150
                     /gene="gabD"
                     /locus_tag="ACHY09_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS3887034.1"
                     /translation="MKPNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4164..5444
                     /gene="gabT"
                     /locus_tag="ACHY09_004140"
     CDS             4164..5444
                     /gene="gabT"
                     /locus_tag="ACHY09_004140"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFS3887035.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYI
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGHKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5682..7082
                     /gene="gabP"
                     /locus_tag="ACHY09_004141"
     CDS             5682..7082
                     /gene="gabP"
                     /locus_tag="ACHY09_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFS3887036.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7103..7765
                     /gene="csiR"
                     /locus_tag="ACHY09_004142"
     CDS             7103..7765
                     /gene="csiR"
                     /locus_tag="ACHY09_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFS3887037.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKRDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7766..8215)
                     /gene="kbp"
                     /locus_tag="ACHY09_004143"
     CDS             complement(7766..8215)
                     /gene="kbp"
                     /locus_tag="ACHY09_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFS3887038.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8299..8457)
                     /gene="yqaE"
                     /locus_tag="ACHY09_004144"
     CDS             complement(8299..8457)
                     /gene="yqaE"
                     /locus_tag="ACHY09_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFS3887039.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1909 a         2210 c         2366 g         1978 t
ORIGIN      
        1 taaatatcaa tattcctggg cgtagcgctc ttcttttagg gaaaactgga gaaccgccga
       61 actatctcta tttgtaattt aatgttatat ctgccccgct aaaacggggc agataatatg
      121 tttagtttac taacggtcat tttgcagtga agccatttac cgttttttat cgaccagata
      181 atctgttctc tgatggtaat ccacccctaa cctgttgctt tagttattca tttcctgtct
      241 cactttgcct taataaccta cgtcaaatgt taccaatttg ttgtttttga tcgcaataag
      301 aaaacaatat gtaactttta tgcgcatttt tcagaaatgt agatattttt agattatggc
      361 tacgaaatga gcatcgccat gtcaccctac atctcataag aggatcgctt ctgatgaatg
      421 cactgaccgc cgtacaaaat aacgctgtcg attcaggcca ggactatagc ggattcactc
      481 tcatcccgtc ggcgcaatct ccgcgtctgc tggaactcac cttcaccgaa cagacgacca
      541 aacagtttct cgaacaggtt gccgaatggc ccgtgcaggc gctggagtac aaatcttttc
      601 tgcgttttcg ggtaggcaaa attcttgacg atctgtgtgc gaaccagctg caaccattgc
      661 tgttgaagac cctgctaaac cgtgctgaag gtgcgctgtt gatcaatgcg gtgggtgtcg
      721 atgatgtgaa gcaagcggat gagatggtga agctggcaac ggcggtagcg catctgattg
      781 gtcgctcaaa tttcgatgcg atgagcggtc agtattacgc gcgattcgtg gtgaaaaatg
      841 ttgataactc agacagctat ctgcgtcagc cgcaccgcgt aatggagctg cacaacgacg
      901 gcacctacgt cgaagagatc accgactatg tgctgatgat gaaaatcgac gagcagaaca
      961 tgcagggtgg aaattcgttg ctgctgcatc tcgatgactg ggaacatctg gaccactatt
     1021 tccgccaccc gctggcgcgt cgcccgatgc gctttgccgc gcctccgagc aaaaacgtca
     1081 gcaaagatgt tttccatcca gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgtt
     1141 atatcgacca gttcgtccag ccaaaagact tcgaagaagg cgtgtggctg agtgagcttt
     1201 ccgatgccat tgaaaccagc aaaggcattc tttctgtgcc cgttcccgtt ggcaaattcc
     1261 tgttgattaa caatctattc tggctgcacg gtcgcgaccg ctttactccg cacccggatc
     1321 tgcgccgtga actgatgcgt cagcgtggct atttcgctta cgccactaac cactaccaga
     1381 cgcatcagta agcgcaaagg aattgagcgg atgtatgatt ttgtgattat tggcggcggc
     1441 atcatcggca tgtcgaccgc catgcaactg attgatgttt atccggatgc ccgcattgcg
     1501 ttgcaggaaa aagagtccgg cccggcctgt caccagacgg gccacaacag tggcgtgatc
     1561 catgccgggg tctattacac gcccggtagc ctgaaggcac agttttgcct ggcgggaaac
     1621 cgcgccacca aagccttttg cgatcaaaac ggcattcgct atgacaactg cggcaagatg
     1681 ctggtcgcca cctctgaact cgaaatggaa cggatgcgcg cgttgtggga acgcacggca
     1741 gcgaacggta tcgagcgcga gtggttaaac gccgaggaac tgcgcgagcg cgaaccgaat
     1801 atcaccgggc tcggcggcat ttttgtgccg tccagcggca ttgtcagcta ccgcgaagtg
     1861 acggcggcga tggcaaaaat cttccaggcc agaggcggcg agattattta taacgccgaa
     1921 gtcagcgccc tcagtgagca taaaaacggc gtggtgatac gtacccgtca gggcggtgaa
     1981 tatgaagcat cgacgctgat tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg
     2041 ctcggcctcg aaccgggctt tattatctgc ccgttccgtg gcgagtattt ccgccttgcg
     2101 ccggagcata accagattgt taaccacctg atttacccca ttcccgaccc cgcgatgcca
     2161 tttttgggcg tgcatctcac ccgcatgatc gatggcagcg tgaccgtcgg gccaaacgcg
     2221 gtgctggctt tcaaacgcga aggctatcgt aagcgcgact tctcatttag cgacacgctg
     2281 gagattttgg gctcgtcggg gattcgccgg gtgctgcaaa accatctacg ctcaggactg
     2341 ggcgagatga aaaactcgct gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt
     2401 ccccggcttt cgttaagcga tctccagccc tggcccgctg gtgtgcgggc gcaggcggta
     2461 tcgccggacg gcaagctgat tgacgatttt ctgtttgtca ccaccccgcg cacgatccac
     2521 acctgcaatg cgccctcccc ggcagcgaca tcagcaattc ctattggtgc gcatattgtc
     2581 agcaaggtac aaacgctgtt ggcaagccag agtaaccccg gacgcacgct gcgagcggca
     2641 cgtagtgtgg atgccttaca cgccgcattt aatcaataac ctttgaaaac aggacgtaac
     2701 aatgaaacct aacgacagta acttattccg ccagcaggcg ttgattaacg gggaatggct
     2761 ggacgccaac aatggcgagg tcatcgacgt caccaatccg gcgaacggcg acaagctggg
     2821 tagcgtaccc aaaatgggcg ctgatgaaac ccgcgccgct atcgacgccg ccaaccgcgc
     2881 tctgcccgcc tggcgtgcgc tcaccgccaa agaacgcgcc accattctgc gcaactggtt
     2941 caatttgatg atggagcatc aggacgattt agcgcgcctg atgaccctag aacagggcaa
     3001 accgctggcc gaagcgaaag gcgaaattag ctacgccgcc tcctttattg agtggtttgc
     3061 cgaagaaggc aaacgcattt atggcgacac cattcctggt catcaggccg ataaacgcct
     3121 gattgttatc aagcagccga ttggcgtcac cgcggctatc acgccgtgga acttcccggc
     3181 ggcgatgatt acccgcaaag ccggtccggc gctggcggca ggctgcacca tggtgctgaa
     3241 gcccgccagt cagacgccgt tctctgcgct ggcgctggcg gagctggcga ttcgcgcagg
     3301 cgttccggct ggcgtattta acgtggtcac cggttcggcg ggcgcggtgg gtaacgaact
     3361 gaccagcaat ccgctggtgc gcaagctgtc gtttaccggc tcgaccgaaa ttggccgcca
     3421 attaatggaa cagtgcgcga aagacatcaa gaaagtgtcg ctggaactgg gcggcaacgc
     3481 gccgtttatc gtctttgacg atgccgacct cgacaaagcc gtggaaggcg cactggcctc
     3541 gaaattccgc aacgccgggc aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg
     3601 cgtgtatgac cgctttgccg aaaaattaca gcaggcggtg agcaaactgc acatcggcga
     3661 cgggctggag aatggcgtca ccatcgggcc gctgattgat gaaaaagcgg tagcaaaagt
     3721 ggaagagcat attgccgatg cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc
     3781 ggatgaacgc ggcggcaact tcttccagcc gaccattctg gtggacgttc cggccaacgc
     3841 caaagtgtcg aaagaagaga cgttcggccc ccttgccccg ctattccgct ttaaagatga
     3901 agccgatgtg attgcgcagg ccaatgacac cgagtttggc ctggccgcct atttctacgc
     3961 ccgtgattta agccgcgtct tccgcgtggg cgaggcgctg gagtacggca tcgtcggcat
     4021 taatactggc attatttcca atgaagtggc cccgttcggt ggcattaaag cctcgggtct
     4081 gggtcgtgaa ggttcgaagt atggcatcga agattactta gaaatcaaat atatgtgcat
     4141 cggtctttaa ctggagaacg cgaatgagca gcaataaaga gttaatgcag cgccgcagtc
     4201 aggcgattcc ccgtggcgtt gggcaaattc accccatttt cgctgaccgc gcggaaaact
     4261 gccgggtgtg ggacgttgaa ggccgtgagt atattgattt cgcgggcggc attgcggtgc
     4321 tcaataccgg gcacctgcat ccgaaagtgg ttgcggcggt ggaagcgcag ttgaaaaagc
     4381 tgtcgcacac ctgcttccag gtgctggctt acgaaccgta tctggagctg tgcgagatta
     4441 tgaatcagaa ggtgccggga gatttcgcca aaaaaacgct gctggtaacg accggttctg
     4501 aagcggtgga gaacgcggtg aaaatcgccc gcgccgccac caaacgcagc gggaccatcg
     4561 cctttagcgg cgcgtatcac gggcgcacgc attacacgct ggcgctgacc ggcaaggtga
     4621 atccgtactc tgcgggcatg ggactgatgc ctgggcacgt ttatcgcgcg ctttatcctt
     4681 gcccgctgca cggcatcagt gaagatgacg ctatcgccag cattcaccga atttttaaaa
     4741 atgacgctgc gccggaagat atcgccgcca tcgtgattga gccggttcag ggcgaaggcg
     4801 gtttctacgc cgcgacgcca gcctttatgc agcgtttacg cgcgctgtgt gacgagcacg
     4861 ggatcatgct gattgccgat gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg
     4921 cgatggagca gatgggcgtt gcgccggatc ttaccacctt tgcgaaatcg atcgcgggcg
     4981 gcttcccgct ggcgggcgtc accgggcgcg cggaagtgat ggatgccgtc gctccaggcg
     5041 gtctgggcgg cacctatgcc ggtaacccga ttgcctgcgt ggcagcgctg gaagtgttaa
     5101 aggtattcga gcaggaaaat ctgctgcaaa aagccaacga cctggggcat aagctgaaag
     5161 acggattgct ggcgatagcc gaaaaacacc cggagatcgg cgatgtacgc gggctgggag
     5221 cgatgattgc catcgagctg tttgaagatg gcgatcacag caaaccggac gccaaactca
     5281 ccgccgagat cgtggcgcgt gcccgcgata aaggcctgat tcttctctcc tgcggcccgt
     5341 attacaacgt gctgcgcatc cttgtaccgc tcaccattga agacgctcag atccgtcagg
     5401 gtctggaaat catcagccag tgttttgatg aggcgaagca gtagatccgt acccatgccg
     5461 gaggcgacgc tgcgcgtctt gtccggccta cgcgaatcca ggtcggataa agcgtttacg
     5521 ctgcatccga caatctgtac gtgaacagga ataaatctat gtcggccgga taaggcggag
     5581 ccgctctccg gtaaaaagaa ccaataacaa ttacacgcgt gacccgacgc gggaaatgtc
     5641 ggggcgctct cccaagtgac acactttcga gaggattcag gatggggcaa tcatcgcaac
     5701 cacatgagtt aggcggcggg ctgaagtcac gccacgtcac catgttgtct attgccggtg
     5761 ttatcggcgc aagtctgttt gtcggttcca gcgtcgccat cgccgaagcg ggcccggcgg
     5821 tattactggc ctatctgttc gccggattac tggtggttat gattatgcgg atgttggcgg
     5881 aaatggcggt tgccacgccc gataccggtt cgttttccac ctatgccgac aaggcaattg
     5941 gccgctgggc gggttatacc atcggctggc tgtactggtg gttttgggta ctggttatcc
     6001 cgctggaagc caacatcgcc gccatgatcc tgcactcgtg ggttccaggc attcccatct
     6061 ggttattttc cctggtcatt accctcgcct tgactggcag taatttatta agcgttaaaa
     6121 actacggcga atttgagttc tggctggcgc tgtgcaaagt catcgctatc ctggccttta
     6181 ttttccttgg tgcagtcgca attagcggtt tttaccctta tgccgaagtg agcgggatct
     6241 caagattgtg ggatagcggc ggctttatgc ccaacggctt cggcgcagta ttaagcgcga
     6301 tgttgatcac catgttctcg tttatgggcg cagaaattgt caccattgcc gccgcggaat
     6361 ctgacacgcc ggaaaaacat attgtccgcg ccaccaactc ggttatctgg cgtatttcta
     6421 tcttctattt atgctctatt tttgtcgtgg tggcattaat accgtggaat atgccggggc
     6481 tgaaagccgt tggatcttat cgctcggttc tggagttgct caatattccc catgcgaaat
     6541 taatcatgga ctgcgtgata ttactttccg taaccagttg cctgaactcg gcgctgtata
     6601 ccgcttccag aatgctctac tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta
     6661 aaatcaaccg cagtaaaacc ccgtatgtgg cggtattact ctcgaccggt gcggcatttt
     6721 taacggtggt ggtgaattat tacgcgcctg cgaaagtgtt taaatttctg atcgatagct
     6781 ccggcgctat cgccctgctg gtttatttag tcattgccgt ttcacaattg cggatgcgca
     6841 aaattctgcg agcagaagga agcgaaattc gcctgcggat gtggctttat ccgtggctca
     6901 cctggctggt catcggtttt ataacctttg tgttggtagt gatgctattc cgcccggcgc
     6961 aacagttaga agtgatttcc accggtttat tagcgatagg gataatctgt accgtgccaa
     7021 ttatggctcg ctggaaaaag ctggtattgt ggcaaaaaac acccgttcat aatacgcgct
     7081 aataatgatc aggagtcaca ccatgaccat tacgtctctg gatggctatc gctggctgaa
     7141 gaacgatatt attcgcggaa actttcaacc ggatgaaaaa ttacggatga gtttgctgac
     7201 atcgcgttat gcacttggcg ttgggccgtt gcgggaagcg ctttcgcaac tggtagcaga
     7261 acgactggtc acggtggtga atcaaaaagg gtatcgggtg gcgtctatgt cagagcaaga
     7321 gctgctcgat attttcgacg cccgcgccaa tatggaagcg atgctggtca gtctggcgat
     7381 tgcccgcggt ggcgatgaat gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa
     7441 gctggaggcc tgtgacgcca gtgagaaaat gcttgatgag tgggatctgc gtcatcaggc
     7501 gtttcatact gcaattgtgg cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg
     7561 gttgtttgat ctggcggcgc gttatcgatt tatctggctg cggcgaacag tgctttcggt
     7621 ggaaatgctg gaagataaac gcgatcagca ccagaccctc actgcggcgg tactggcgcg
     7681 agataccgcg cgcgccagtg agttaatgcg ccagcattta ctgacgccaa ttcccattat
     7741 ccagcaggcg atggctggca attaactact cttcaggaat acgcaacact tgccccgggt
     7801 aaattttatc cgggcttttg agcatcggtt tattcgcttc gaagatttta ttgtacagat
     7861 tagcgttacc gtagacctgt ttggaaatgg cactcagagt gtcgccagac ttaacggtat
     7921 aaaactggct ggcagcggct ggtgtcgccg ttttcacctg atcatcgaca ctggcaatac
     7981 cggaaatatt ccccaccgca acaaggattt tctccttcgc ctcctgactt aagccgtcac
     8041 ccgtgaccgt cgctttgccg tcggcgattt gaatgttcac tttatcggca tcaggtattc
     8101 cggttttgtt cagatgctcc tgcaccttct tcgcctgatc gtctttatcg tgctgacctg
     8161 taaccgcgtc ccagagtttt tctccggcat ctttcacaaa attgaacaga cccatagcta
     8221 cctcattcgt taacggaaac actcagaaag tgtagcagag gaacgcggcg cgttaacgcc
     8281 gcgggggtga acacaggttt aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg
     8341 gatataccca agcagtgtca gcagaatatt aataatgaac gcccaaccga accctttacc
     8401 gagcagcacg ccgagcggcg gcagaatgat ggtgatgacg attctccaga aacccatatg
     8461 tac
//
LOCUS       DAZUCC010000202         8441 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZUCC010000202 DAZUCC010000000
VERSION     DAZUCC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.203"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..1243
                     /locus_tag="ACHY09_004145"
     CDS             23..1243
                     /locus_tag="ACHY09_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HFS3887040.1"
                     /translation="MKYHSEPLVPHKSALMQMPANLLAEDVCLPAAIIKKQALENNIT
                     WMQRYADARGVSLAPHGKTTMTPWIFQAQQAAGAWAIGVGSAWQAGAAMASGIQRVLM
                     VNQLVGKANMQLIAQLQRHYPTVDFICCIDSIENARALSAFFASQQQTLNVMIELGVP
                     GGRCGCRSRDAALTLAKQAAQLPGLRLRGLELYEGVLHGDDPQPQVEALLRDAAQLAC
                     DMASLVDGEFILTGAGTVWYDVVCNIWLAAEKPDNCRIVIRPGCYITHDTGIYDAAQQ
                     QLLARDPVACDLAGDLTSALELVAMVQSVPEADRAVVNFGKRDCAFDAGLPQPVAHYR
                     NGKSLAFDPQAIRSTGIMDQHCMLQLSANSDVQVGDILVFGTSHPCLTFDKWKTLLLT
                     DDDYNVLAELDTLF"
     gene            1511..3661
                     /gene="btsT"
                     /locus_tag="ACHY09_004146"
     CDS             1511..3661
                     /gene="btsT"
                     /locus_tag="ACHY09_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFS3887041.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            3711..3914
                     /gene="yjiX"
                     /locus_tag="ACHY09_004147"
     CDS             3711..3914
                     /gene="yjiX"
                     /locus_tag="ACHY09_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFS3887042.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            3925..4881
                     /gene="yjiA"
                     /locus_tag="ACHY09_004148"
     CDS             3925..4881
                     /gene="yjiA"
                     /locus_tag="ACHY09_004148"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFS3887043.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEVEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPSRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDKIRAAFAGLRK"
     gene            complement(4915..5217)
                     /locus_tag="ACHY09_004149"
     CDS             complement(4915..5217)
                     /locus_tag="ACHY09_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFS3887044.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(5232..5333)
                     /locus_tag="ACHY09_004150"
                     /pseudo
     CDS             complement(5232..5333)
                     /locus_tag="ACHY09_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            5530..6696
                     /locus_tag="ACHY09_004151"
     CDS             5530..6696
                     /locus_tag="ACHY09_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS3887045.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVDREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            6760..8379
                     /locus_tag="ACHY09_004152"
     CDS             6760..8379
                     /locus_tag="ACHY09_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFS3887046.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEVLGYGE"
BASE COUNT         2022 a         2204 c         2229 g         1986 t
ORIGIN      
        1 aaaaacttaa aaggtaaatg ttatgaaata ccactcagaa cctctggttc cccataaatc
       61 tgccctgatg caaatgcccg caaatctcct tgccgaagat gtctgtttac ctgccgcgat
      121 cattaaaaag caggccctgg agaacaacat tacgtggatg caacgctacg ctgacgcgcg
      181 cggcgtttca ctggcaccgc acggtaaaac caccatgacg ccgtggattt ttcaggcgca
      241 gcaggcggct ggtgcctggg caataggtgt cggtagcgca tggcaggctg gtgcggcaat
      301 ggcgagcggt attcagcgag tgctgatggt taaccagctg gtcggcaagg cgaatatgca
      361 gttgattgcc cagttgcaac gacattaccc gacggtcgat tttatctgct gtatcgacag
      421 cattgagaac gcccgcgctt tgtctgcctt ttttgccagt cagcaacaga cactgaacgt
      481 gatgattgag ttgggtgtcc ccggcgggcg ctgtggttgc cgctcaaggg atgccgcact
      541 gacactggct aaacaggcag cccaattacc ggggttacgc ctgcgggggc ttgagttata
      601 tgaaggcgtt ttacacggtg acgatccaca accgcaggta gaagctctgc tgcgggatgc
      661 cgcacaactg gcctgtgata tggcaagtct ggttgatggc gagtttattc tcaccggagc
      721 aggcacggtc tggtatgacg tggtgtgcaa tatctggctg gcggcagaaa agcctgataa
      781 ctgccgcatt gttattcgcc ctggctgcta catcactcac gacaccggga tctacgacgc
      841 ggcacaacaa cagttgcttg cccgtgatcc tgtggcctgc gatctggcag gcgatctcac
      901 atctgcgctg gaactggtcg ccatggtgca atcggtgccg gaagctgatc gtgcggtggt
      961 aaattttggt aagcgtgatt gcgccttcga cgccggactg ccgcaaccag tcgcccacta
     1021 ccgcaacggc aaatcactgg catttgatcc acaggcgatt cgcagtacag gtattatgga
     1081 tcagcactgc atgttgcaac tgtcagctaa tagcgacgtg caagtggggg atattctggt
     1141 gtttggcaca tcgcatccgt gcctgacctt cgacaaatgg aaaacgttgt tattgactga
     1201 tgacgactac aacgtactgg cagaattaga cactctcttc taagaacgcc ccttcggtta
     1261 attatcgaag gggcattttt attgtgatct caatcacata tccacccgac gaataattct
     1321 tgcccaaccg ctcaccgcca ttcctgaccg cttacatccc taaaataacc actcagttat
     1381 ttaccttact ttacgcgcgc gtaactctgg caacatcact gcaggatagc ggtcaattta
     1441 cctcctcaaa cgcaacgcaa acctagaacg gcttcggcca actattaatc aatacatgcc
     1501 aggttttact atggatacta aaaagatatt caagcacata ccctgggtga ttctcggaat
     1561 catcggtgca ttctgcctcg cggtagttgc attacgtcgg ggggagcacg tcagtgccct
     1621 gtggatcgtg gtcgcctctg tgtcggtgta tctggtggct tatcgctact acagtctgta
     1681 catcgcccag aaggtgatga aactcgaccc cacgcgcgcg acgcctgcgg ttattaacaa
     1741 cgacggtctg aactatgttc cgaccaaccg ttacgtgttg tttggtcacc acttcgccgc
     1801 tatcgccggt gctggtccgc tggttggtcc ggttctcgcc gcgcagatgg gctacctgcc
     1861 tggcacgctg tggctgctgg cgggggtagt gctggccggt gcggtacagg actttatggt
     1921 gctgtttatc tcctctcgcc gtaacggcgc atctctcggt gagatgatca aagaagagat
     1981 gggaccagta ccggggacta tcgcgctgtt tggctgtttc ttaatcatga tcatcatcct
     2041 tgccgtcctg gcgctgattg tggttaaagc cctggccgaa agtccgtggg gtgtcttcac
     2101 cgtttgctca accgtaccaa ttgcgctgtt tatgggtatc tacatgcgct ttatccgtcc
     2161 ggggcgtgtg ggtgaagtct ctgtcattgg tatcgtgctg ctggttgcct ctatctactt
     2221 cggtggcgtg attgctcacg atccgtactg gggtccggca ctgaccttta aagacaccac
     2281 cattaccttc gcgctgattg gctatgcgtt tgtttccgcg ctgctgccgg tgtggctgat
     2341 cctcgcacca cgcgactatc tggcaacctt cctgaaaatc ggcgttatcg tcggcctggc
     2401 gctgggtatc gtggtgctga acccggaact gaaaatgcct gcgatgaccc agtacattga
     2461 cggtactggc ccgctgtgga aaggcgctct gttcccgttc ctgttcatca ccatcgcctg
     2521 tggtgcggta tctggcttcc acgcgctgat ctcttccggt acgacgccga aactgctggc
     2581 taacgaaacc gacgcgcgtt tcattggcta cggcgcaatg ctgatggagt ccttcgtggc
     2641 gattatggcg ctggttgctg cgtccatcat cgaaccgggt ctttacttcg cgatgaacac
     2701 cccgcctgct ggccttggca tcaccatgcc taacctgcat gaaatgggtg gcgagaacgc
     2761 gccgatcatc atggcgcagt tgaaagacgt taccgcacac gcggcggcga ccgtcagctc
     2821 ctggggcttc gtgatttcgc cagagcagat cctgcaaacc gcgaaagaca ttggtgaacc
     2881 ttctgtcctg aaccgtgcag gtggtgcgcc gacgctggcg gtaggtatcg cgcacgtgtt
     2941 ccacaaagtg ctgccgatgg ctgacatggg cttctggtat cacttcggta ttctgttcga
     3001 agccctgttc atcctgaccg cgctggatgc gggtacccgt tctggccgct ttatgctgca
     3061 agacctgctg ggtaacttca tcccgttcct gaaaaaaacc gactctctgg ttgctggtat
     3121 catcggtact gcgggctgtg tgggtctgtg gggctacctg ctgtatcagg gcgtggtcga
     3181 tccgctgggc ggcgttaaga gcctgtggcc gctgttcggt atttccaacc agatgctggc
     3241 agccgtagca ctggtactgg gcaccgttgt gctgattaag atgaagcgca cccaatacat
     3301 ctgggtaact gttgttccgg ctgtatggct gcttatctgc accacctggg cgctgggcct
     3361 gaaactgttc agcaccaacc cgcagatgga aggcttcttc tacatggcaa gccagtacaa
     3421 agagaagatt gctaacggta ctgacctgac ggcacagcag attgccaaca tgaaccacat
     3481 cgttgtgaac aactacacta acgcgggtct gagtattctg ttcctgattg tggtgtacag
     3541 catcatcttc tacggtttca aaacctggct tgcggtgcgt aacagcgaca aacgtactga
     3601 caaagaaact ccgtacgttc caatcccgga aggcggcgtg aagatctctt cgcaccacta
     3661 accgtgttta gccccgcttc ggcggggctt tgttcttatc agagtgaact atgtttggta
     3721 acttaggaca ggcaaaaaaa tatctcggcc aggcggcgaa gatgttgatt ggcattccag
     3781 actacgacaa ctacgtcgag catatgaaga ccaaccatcc cgacaagccg tacatgagct
     3841 atgaagaatt cttccgcgaa cgccagaacg cgcgctacgg cggcgatggt aaaggcggta
     3901 tgcgctgttg ttaatggaga gagcatgaac ccgattgcag ttaccctact caccggtttt
     3961 ttaggcgcgg gcaaaaccac cctgctgcgc catattctta atgaacagca cggctacaaa
     4021 attgccgtga ttgaaaacga attcggcgaa gtctccgttg atgatcaact gattggcgac
     4081 cgcgccacgc agatcaaaac gctgaccaac ggctgcatct gctgttcgcg ctccaacgag
     4141 ctggaggacg cgctactcga cctgctggac aatctcgaca agggcaatat tcagttcgac
     4201 cgtctggtca ttgaatgcac cggcatggcc gatcccggcc cgattattca gacctttttc
     4261 tcccatgaga ttttatgcca gcgttacctg ctggacggcg tgattgcgct ggtggatgcg
     4321 gtacatgccg atgagcagat gaaccagttc accatcgccc agtcccaggt gggctacgct
     4381 gaccgtattc tgctgactaa aaccgacgtc gcaggcgaag tagagaaact gcgtgaacgc
     4441 ctggcgcgca tcaacgcccg cgcaccggtc tacaccgtca cccacggtga catcgacctg
     4501 ggcctgttgt tcaacaccaa cggttttatg ctggaagaaa acgtcgtcag caccaaaccg
     4561 cgtttccact ttatcgcgga taaacaaaac gatatttcgt cgattgtggt ggaactggat
     4621 tacccggtag atatcagcga agtttcccgc gtgatggaaa acctgctgct ggaatcggcg
     4681 gataaactac tgcgttacaa agggatgctg tggattgacg gcgaacctag ccgcctgctg
     4741 ttccagggcg tccaacgcct ctacagcgcc gactgggacc gcccgtgggg cgatgaaaca
     4801 ccgcatagta caatggtgtt tatcgggatt cagctgccgg aggataagat tcgggcggcg
     4861 tttgcggggt tgaggaagta attttagatg atggccgcca tgtggcggcc tgaattattt
     4921 tttattctga tcgcgcagca tcgcttcacg taaaattgcg ttcaggcgag tttgatagcc
     4981 tttacctggt cgcttcaacc attccatcac atcagcatca atacgcaccg aagcctgagt
     5041 tttcaatggc cgataaaact taccgcgttc tgcgttagac cattgctcat cgctggaagg
     5101 tgggatatca ctgtaatcaa tatcttcgtc ggatttattc gccagcgcct tcagttcggc
     5161 ctcatgctgc acatcgagtg tagaggaggt ccctcgttta tgtttaacca tgctcatagc
     5221 gattccgttc gttgtggatc atcaaacact aacaccgcat cttcaaagcg gatgccatgt
     5281 ttcacccgat tacctttggc cttatttgca tcccattcaa actccattgg catagttgcc
     5341 atccctaatt gtatatacaa aaatgtatgt acaattattc tgcaattata gcgttgatgt
     5401 tggaaatagc atcacgtttc gaataatcaa ccatgaaatt gacaatgtgt gagttggact
     5461 tgattcatcc aatagaattt ctcgcctgac atggcacact aaacacctcg ctgtcgttaa
     5521 gggattaccg tgaaaaacta caaagtcatt accccgcttt ttcctaccta tgcgcaagtt
     5581 aaggctatga tgaaagccgt ctcaggctat tccctgaaag cggtgcgcaa tatgattact
     5641 gccattcacg agcaaaccgg aacgccgcaa aaacccgttg actggtcgga acctgacctg
     5701 tggatcagcg aacgcttaac gggtgaagat gccgacatcg cccgtcgtat ttgggatacc
     5761 gataatcaca tattgaaccc gcgccacagc tacggctgct acttattcct caactatccc
     5821 cagtttgagc tgatggaaag cacgccagac gatacctggc agcccacctc gcgcgggcaa
     5881 aaattcctgc aagatgacga aaaaacgctg cgttcactgg acgatcagga aggcattctg
     5941 caacttcttg aactattagc tggccgcgaa atgtcgcgcc gcgccgattt attgccggaa
     6001 tggcaggcct ttttgcatca gcattcgaaa ttcgcgtctg caagttcagt gaaaagtacg
     6061 ctctactccc gtctctataa ccttatcgac cgtgacatgg ttgaccgtga aggcatgagc
     6121 tatcgcatca ccgataccgg gcgcgcctgg cttggaaaag cgttgccggc acgaaaaagc
     6181 gatccacgta aagagctgct ggaagcggtg aaacgctaca acgagcagca aaaagagctg
     6241 ctgcgcgaac aaatcagcac gatgaacccg tacaaatttg aacaactggt tggtcaatta
     6301 ctggaagcaa tgggctacga gcaggtcgaa gtgaccaaag cctctggcga caaaggtgtg
     6361 gacgtgattg gccaggtgca ggtcggcatc accactatta ccgaagtggt gcaggtcaaa
     6421 agaatgcaga acactatcac tcgcccatat atcgaccagt tgcgcggggc gttgccttac
     6481 cacaaagcca ttcgcggtac actcatcacc accgggaaat ttgcggcgaa atgtgcagaa
     6541 gcggcgctct ttccgggtgc cgcgcccatt acgcttatcg acggcgatcg tttactggaa
     6601 ctgttaatcg aaaataacgt cggcattcgc cgcagcaatg cagtggaatt gctggatgtg
     6661 gatttgcagt tgtttgatga gctggagatg gattaaaggg cgaaatccgc cgcctctacc
     6721 attacaatag cgacccaatt tatctgaaca gagttcatca tggccaaagc accaactaaa
     6781 aaagccaaag caaagaaagg ctttgaagaa acattatggg atacagcaaa ccagcttcgc
     6841 ggtagcgttg agtcctccga atacaagcac gtggtgttga gcctcgtgtt cctgaaattc
     6901 atcagcgata agtttgaagc acgccgcaaa aaaatgattg ccgatgggca agcagacttc
     6961 cttgagatgg aagtgttcta ccagcaggac aacattttct acctgccgga agaagcgcgc
     7021 tggtctttta tcaaacaaaa tgccaaacag gacgatattg cggtccgtat tgacaccgcc
     7081 ctctcgacca tagagaaacg taacccaacg ctgaaaggcg cgctgccaga caactacttc
     7141 agccgtcaga atctggaaac caaaaaactg gcgtcactga ttgataccat cgacaacatc
     7201 gaaacgctgg cacacgaaac tgacgttgaa acgttatcga aagaagacct ggtcggacgc
     7261 gtttacgaat acttcctcgg taagtttgcc gcaactgaag gcaaaggcgg tggtgagttc
     7321 tacacgccca aatgtgtggt tacgctgtta actgaaatgc tcgaaccctt ccagggcaaa
     7381 atttatgacc cgtgctgcgg ctcggcaggg atgttcgtgc agtcggtgaa gtttgtcgag
     7441 agccatcagg gtaaaagtcg tgatatcgcg ctgtatggtc aggaactgac agccacgacg
     7501 tataaactgg caaaaatgaa cctcgccatt cgcggtcttt cagctaacct cggcgaacga
     7561 ccggcagaca ccttctttag cgaccagcac ccggacctga aagccgacta cattctggcg
     7621 aacccgccct tcaacctgaa agactggcgt aatgaagccg aattaaccaa agacccacgt
     7681 tttgctgggt atcgtatgcc gccaaccggt aacgccaact acggctggat tttgcatatg
     7741 ctctccaagc tgtcggctaa cggcacagca ggttttgtgc tggcaaacgg ttcgatgagt
     7801 tctaacacca gcggtgaagg cgagatccgt gcacagatga tcgaaaatga tctgattgac
     7861 tgtatgatcg ccctacctgg acagttgttc tacaccacgc agatcccggt gtgtttatgg
     7921 tttatgacca aatcgaaggc tgccgatccg gccaaaggtt atcgtgatcg tcagggcgag
     7981 acgctgttta ttgatgcgcg taacctcggc accatgatta gccgcacaac taaagagtta
     8041 acggcggaag atattgccac catcgccgat acctaccatg catggcgcag tacgccggaa
     8101 gaactggctg cacggattgc gcgtggtgac agcaagctgg aaaaatatga agaccaggct
     8161 ggcttctgca aagttgcgac cctgcaagac attaaagata acgactacgt tctgacaccg
     8221 ggccgctatg taggtgcagc cgagcaggaa gaggacggcg tggcatttga gacgaaaatg
     8281 cgcgaattgt cgaagacgtt gtttgagcag atgaagcagg cggaagaact ggatcgtgcg
     8341 attcgccaga atctggaggt gctgggttat ggggagtaag tggatagaag tatcactggg
     8401 cgagatttct gaaaaaattg gagatggaat ccacggaacg c
//
LOCUS       DAZUCC010000203         8408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZUCC010000203 DAZUCC010000000
VERSION     DAZUCC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.204"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..2476
                     /gene="ppsA"
                     /locus_tag="ACHY09_004153"
     CDS             98..2476
                     /gene="ppsA"
                     /locus_tag="ACHY09_004153"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFS3887047.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2533..4179)
                     /gene="fadK"
                     /locus_tag="ACHY09_004154"
     CDS             complement(2533..4179)
                     /gene="fadK"
                     /locus_tag="ACHY09_004154"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFS3887048.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPAACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFIHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEDVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4236..4529)
                     /locus_tag="ACHY09_004155"
     CDS             complement(4236..4529)
                     /locus_tag="ACHY09_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFS3887049.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(4526..5815)
                     /locus_tag="ACHY09_004156"
     CDS             complement(4526..5815)
                     /locus_tag="ACHY09_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS3887050.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLDAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5870..6808)
                     /locus_tag="ACHY09_004157"
     CDS             complement(5870..6808)
                     /locus_tag="ACHY09_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFS3887051.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQTV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSN
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6828..7592)
                     /locus_tag="ACHY09_004158"
     CDS             complement(6828..7592)
                     /locus_tag="ACHY09_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFS3887052.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7908..>8408
                     /locus_tag="ACHY09_004159"
     CDS             7908..>8408
                     /locus_tag="ACHY09_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS3887053.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKN"
BASE COUNT         1999 a         2179 c         2184 g         2046 t
ORIGIN      
        1 aacgattcac cgtttttttc atccggttaa atatgcaaag ataaatgcgc agaaatgtgt
       61 ttctcaaacc gttcatttat cacaaaagga ttgttcgatg tccaacaatg gctcgtcacc
      121 gctggtgctt tggtataacc aactcggcat gaatgatgta gacagggttg ggggcaaaaa
      181 tgcctccctg ggtgaaatga ttactaacct ttccggaatg ggtgtttccg ttccgaatgg
      241 tttcgccaca accgccgacg cgtttaacca gtttctggac caaagcggcg taaaccagcg
      301 catttatgaa ctgctggata aaacggatat tgacgatgtt acccagcttg cgaaagcggg
      361 cgcgcaaatc cgccagtgga ttatcgacac tcccttccag cctgagctgg aaaacgccat
      421 ccgcgaagcc tatgcacagc tttccgccga tgacgaaaac gcctcgtttg cggtgcgttc
      481 ctccgctact gcagaagata tgccggacgc ttcttttgcc ggtcagcagg aaactttcct
      541 caacgttcag ggttttgacg ccgttctcgt ggctgtgaaa catgtatttg cttctctgtt
      601 taacgatcgc gccatctctt atcgtgtgca ccagggttac gatcaccgtg gcgtggcgct
      661 gtccgccggt gttcagcgga tggtgcgctc cgacctcgca tcttctggcg tgatgttctc
      721 cattgatacc gaatccggct ttgaccaggt ggtgtttatc acttccgcat ggggccttgg
      781 tgaaatggtc gtgcagggtg cggttaaccc ggatgagttt tacgtgcaca aaccgacact
      841 ggcggcgaat cgcccggcta tcgtgcgccg caccatgggg tcgaaaaaaa tccgcatggt
      901 ttacgcgccg acccaggagc acggcaagca ggttaaaatc gaggacgtac cgcaagaaca
      961 gcgtgacatc ttctcgctga ccaacgaaga agtgcaggaa ctggcaaaac aggccgtaca
     1021 aattgagaaa cactacggtc gcccaatgga tattgagtgg gcgaaagatg gccacaccgg
     1081 taaactgttc attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc aggtcatgga
     1141 gcgttatacg ctgcattcac agggtaagat tatcgccgaa ggccgtgcta tcggtcatcg
     1201 catcggtgcg ggtccggtga aagtcatcca tgacatcagc gaaatgaacc gcatcgaacc
     1261 tggagacgtg ctggtcactg acatgaccga cccggactgg gaaccgatca tgaagaaagc
     1321 atctgccatc gtcaccaacc gtggcggtcg tacctgtcac gcggcgatca tcgctcgtga
     1381 actcggcatt ccggcggtag tgggctgtgg tgatgcaaca gaacggatga aagacggtga
     1441 gaacgtcact gtttcttgtg ccgaaggtga taccggttac gtctatgcgg agttgctgga
     1501 atttagcgtg aaaagctcca gcgtagaaac gatgcctgat ctgccgttga aggtgatgat
     1561 gaacgtcggt aacccggacc gtgctttcga cttcgcctgc ctgccgaacg aaggcgtcgg
     1621 ccttgcgcgt ctggaattta tcatcaaccg tatgattggc gtccacccac gcgcactgct
     1681 tgagtttgac gatcaggaac cgcagttgca aaacgaaatc cgcgagatga tgaaaggttt
     1741 tgattctccg cgtgaatttt acgttgggcg tctgactgaa gggatcgcga cgctgggtgc
     1801 cgcgttttat ccgaagcgcg tcattgtccg tctctctgat tttaaatcga acgaatatgc
     1861 caacctggtc ggtggtgagc gttacgagcc agatgaagag aacccgatgc tcggcttccg
     1921 tggcgcgggc cgctatgttt ccgacagctt ccgcgactgc ttcgcgctgg agtgtgaagc
     1981 agtgaaacgt gtgcgcaacg acatggggct gaccaacgtt gagatcatga tcccgttcgt
     2041 gcgtaccgta gatcaggcga aagcggtggt tgaggaactg gcgcgtcagg ggctgaaacg
     2101 tggtgagaac gggctgaaaa tcatcatgat gtgtgaaatt ccgtccaacg ccttgctggc
     2161 agagcagttc ctcgaatatt tcgacggctt ctcaattggc tcaaacgaca tgacacagct
     2221 ggcgctcggt ctggaccgtg actccggagt ggtgtctgaa ctgttcgatg agcgcaacga
     2281 tgcggtgaaa gcactgctgt cgatggcgat tcgtgccgcg aagaaacagg gcaaatatgt
     2341 cgggatttgc ggtcagggtc cgtccgacca cgaagacttt gccgcatggt tgatggaaga
     2401 ggggatcgat agcctgtctc tgaacccgga taccgtggtg caaacctggt taagcctggc
     2461 tgaactgaag aaataaaata aatccccggc ggcgttttgt cgccgggttt atgtgatccc
     2521 cgaagatgaa acttattcaa tctcttcaca gacatcctga gttaatcgcc gcataatatc
     2581 tttccttaac aaaaactttt gtattttacc tgaggctgtg cgcggtagtt tttcgattac
     2641 cacgatatgt tcaggatatt tatatttcgc gacccgttta cggctaaaaa atgccactac
     2701 atcttccagc gataatgaat gatgcggtgc tttcagcacg acataagcgc atgaacgttc
     2761 acctaagcgt tcatcgggca ttgcaaccac acaagcatcg tgaattttag gatgctgcaa
     2821 taaaatatct tctacttcac ggctgctaat attttcgccg ccgcggacaa taatatcttt
     2881 cttgcgcccg gttattttta tatagccagc ctcatccatg cggcagagat cgccgctgta
     2941 ataccagcct tcttcatcca gcgcacgggc ggttaattca ggttcatcaa aataccccat
     3001 aaacacattg gggccacgcg aggcttcttc accttcgcaa cctggcggta aagtcttgcg
     3061 tgcgtcatcg accactttaa tctctacacc tgcggcagcg taaccatcgg tgtgtataaa
     3121 gcgcgacaaa ggatcatcga gattcaccac cgcatgcggc gaactttctg tggaaccata
     3181 aacacttaat aatttaatgc cgcgctgctg gcattcacgc gccacttttt tggggattgt
     3241 ggtaccgccg caaagaaaga aacgcagcgc tgaaaggtct gcgggctgtt tctccagtaa
     3301 attcaaaaga tcatagacaa acggcgttgc gccgagcata caggtgcaac gctgctgctc
     3361 aagcagcgcg agacacgcag caggagtgaa aatatctaac aacacgctgc gagcaccaat
     3421 caaaaaaggt gctgttacgc catgcagaaa gcccgttgcg tgaccaagtg gcgcaggcat
     3481 cataaagaca tcctgccagg tcagatttag ccgcgcgcaa taagcccgct cactggcgag
     3541 aatattgtta tgcgttagca tcacgccctt tggcagaccc tcggttccgg aggtaaacag
     3601 caccgcggct aattcatcgc cgtggaccgt tatcgccgtg gtcagaggag tattgtcggc
     3661 gataatctga cttaatgaga gagcagaggt ggcgggagcc agtttgtcca cgccgacaat
     3721 ttgttgtagt tgtggaagct gattttgcag cggcaggatt aaatctaccg gacgcgtttg
     3781 tttaaacaac gtcggtgcaa agaacatttt tgcctgacac ttattgagca cccacaccag
     3841 ttctgcttct cgccaggaag gcaacagcgg cacggaaacc gcaccggttt tcaggcaggc
     3901 aagatagata acggtaaatt cacaccagcc tggcagttga aatgcgatgc gatcgcctga
     3961 ttcaataccc ttcgctaaca tccagtttgc cagacagctc gcggcgtgat cgagcgcgct
     4021 ataagtgtac gttgcaccat gattgtcgac cacggcaatt ttgtctggca tcgcacgagc
     4081 ggtctgctgc cagtaatcgg ccagtgaagc atcgccccat aacccttgct gacgatacgc
     4141 cgcacgacgt tgttcgttga acgttaatgt cactttcatt ttggactctc gaaacagaac
     4201 atcaggcccg agatgcgggc ctgtgggatg catgattagc cgtagcgaaa ttcaataccg
     4261 aaggtgcctg cgggatattg ccactgttcg agaatagtgt tgccgcacag cacccgacag
     4321 gtgccgcact ccagacaacc ggcggaatcg aaatgaatgt ttcctgcgtc atcctgctta
     4381 taaagtccgg cagggcaggc tttcattaat ttacggaatt cattgatatc gggatttgcc
     4441 gccagaatga tatgcgggtg gccctcatca acatggaatt tatttacgcc taatttgatg
     4501 tcgacgttaa ccgtagcgtt ctggctcata gcgcggttgc tcccttaatg ccatctttca
     4561 gcaagttgat cagtccaatt ttcttcgcgt gtcccatgat cattttgcgt accggttggt
     4621 taggtttgcc gtcgatggtg aacatctcgt tcatgatgtc ggcgaccatt cgtgggtatt
     4681 ggctaaacag gcgcgggttt tccatcagcg ccgggatctt gcgaaaatgc tgcatatcac
     4741 gcatgacgca gctttgttcc agctcacgtt tgtattgcgc aagactgctg gcggagaaat
     4801 ccgcgcgttc tttggcggcg atcactgttg tggcggcagc ctgagccgat gcaatggcta
     4861 aatccatgcc gcggaccgta aaacccaaat tcaggcagaa gcctgcggcg tcaccaacga
     4921 tcatcacgcc atcgttaacc agctgcggca ccattgccag accgccctcc ggcaccatat
     4981 gcgcggagta ttcaagcagt ttgccgccgc taatcagcgg gcgaatggcg gggtgttgtt
     5041 taaaatcttc cagcatttgc ggcacacttt tttgcgcatg ggcgatatca cccaatccac
     5101 aaaccagccc caacgatacg gaatctttgt tggtatagag gaatccgccg cccatcaggc
     5161 cgtcagaagg ggaaccggca aacagccagg cggcaccttc ctcgcccgta acattaaagc
     5221 gatcgttgat ctgttctggt gtcaggccta taacctcttt aacaccaaca gcgtaatgat
     5281 gcggatcgga agcggaaacc attcccagcg agcggccaag catcgagtta acgccatcag
     5341 ccagaatcac cacattcgct tcgagaatat catccccggc ctggacgcca gtgaccttgt
     5401 ttccttcacg aaccagtgca tcgacgcgta cgcccgggat aaactgcgcg cccgcctgct
     5461 cggcttgttc catcaaccac gggtccagac gattacgcaa tacggtataa gacgcgtgtt
     5521 gcggaacatc tggttgctcg cggtgaaaat cgagggtaac ggcgctttct tcggttaaga
     5581 aggagatttt ctcgcgtgtg accttgcgtt ctaccggcgc tgatgctgca aagcctggaa
     5641 taattgcgtc aagtgtgtgg gcataaagac gcccgccggt catgttttta catccggcac
     5701 tgtcgccgcg ttctatcacc aggacatcca gcccggctcg cgccatgacc agtgcggcaa
     5761 cgctaccggc aacgcccgca ccgaccacaa tggcatcaaa tttgtcatcc gacatgcgtt
     5821 atgctccctt cccgctgcgt ttacagcggg agatgtggtt gaaagggaat cagcgactca
     5881 actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg tagtcggcat
     5941 agttgaagat tggcgcattt ttatctttat taatggcgac aatcactttt gcgccgttgc
     6001 cgccaaccat atgctggatc tgcccggaga tccccagcgt caggtagaga tcggatttca
     6061 gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg ccttcggcaa
     6121 ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg accattttca
     6181 ggtcatcctg cgctgccaga ccacgaccga cgccaaccac acgttttgct ttgctcaggt
     6241 ccacgctgct tgccgctttc gcacggcgtt cgcgacagag aatttcatga cgcggggcaa
     6301 cataaggtac cgtttctgtc ggacactgat tagaggtatc actggtgcac ggttcctgaa
     6361 caccgggcgc aagggtaatg atcgccagcg ggctattgat cttttcctgt gcgaacgcta
     6421 acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc accgcagtgg
     6481 catcgttcac cagcgccgca ttcagttgca cacttaaccg tgcagccaga gctttaccgc
     6541 gtttcgtcgc ggccagcagc aacatagaag ggtgtttatc tttcagcagg gcagcaatgc
     6601 tttcggcgta attttcagta cgttgcagcg cgtcgttttg cgcaagaaca taaatgcatt
     6661 ttggcccata aggcataact gtctgcgcct ggtcggtatt ttgtacaatg gcatacacct
     6721 gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga ttatcgctaa
     6781 agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct
     6841 ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc gagaatgatg
     6901 tgcttacgtt ctgtttgcgg cggtacgcga atgccggtca gttcagcaag tggtgcgctc
     6961 tggctccagc caatatcact tgcctgccac tgatttaccg gttttttacc cgcgccgagg
     7021 atggctttca tcgaaggaat acgtggtacg ttaatatcgg aggtgacgca gagcacggct
     7081 ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat caccagtgta
     7141 ttgccctgac gctgaatggc actcactgca ttaatcaccg gaagttgcag aatttctccg
     7201 accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa
     7261 tcgaagccga tcttttcaac tgctgtcgcc agcgcctttg cggtatcgag aggcagtgca
     7321 tgctcaagtt gtgcatcctg caccatatac aggctgtgcg gcccgcggga tagcacgtct
     7381 ttgcgcactt tcgagttctg caacaaagag ccgccgacgg tcagcgcggc tatctcatcg
     7441 tcatctgttg caagctggct tgcagcttca atggcattga gatcgaactg gctgattttg
     7501 gcgtcggcat tgtcgaaatt cagggtatat tctggagtga caacaatgtc ctgttcttca
     7561 ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg catacggaac
     7621 catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt gcggggtttt
     7681 cgacggagca agctgttttc cttgccaaca atcttgcaaa aaaaatgcga tcgctaaaac
     7741 aaaagtgcaa tttgcggcaa tgttgttatt ccatgaccgt acaatcttgc attcagtgta
     7801 aataatcttg tttagctctc gctaaacgcg gttgtataac cgcaatacac gccgtatttt
     7861 atccggcata tattgttttt gcttgctcat ggaataggga ggttgtcatg tatcaacgct
     7921 gttttgataa cgccagcgaa acgctgtttg tcgccggtaa aacgccacgg ctttcacgtt
     7981 ttgcatttag cgatgatcca aaatgggagt ccggacatca cgttcatgac aatgaaaccg
     8041 agttgattta cgtcaagaaa ggggtagcaa ggtttaccat cgattcttcg ttatatgtcg
     8101 cgcatgccga tgacattgtg gtgatagaac gtggcaggtt gcatgcggtg gcctctgacg
     8161 ttaacgatcc agcaacgacg tgtacctgtg cgctgtacgg ctttcagttt cagggggctg
     8221 aggaaaatca gctactgcaa ccgcattctt gcccggtaat tgccgcaggg cagggaaaag
     8281 aagtcattaa aaccttattt aatgagctaa gtgtgatttt gccgcaaagt aaaaatagcc
     8341 aaacatcttc gttatgggac gcatttgcct atacgttagc aattctttac tatgaaaact
     8401 ttaaaaat
//
LOCUS       DAZUCC010000204         8401 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZUCC010000204 DAZUCC010000000
VERSION     DAZUCC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.205"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..821
                     /locus_tag="ACHY09_004160"
     CDS             <1..821
                     /locus_tag="ACHY09_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS3887054.1"
                     /translation="TVAVLVPSLTDKTSSRFMQSLQQVLNKNEFQLLLGCHEYNQLKE
                     AEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPFSPAQITIETAFFE
                     ASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSYYQNSDLVITMPGAAS
                     LQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDSC
                     DQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1024..1704
                     /locus_tag="ACHY09_004161"
                     /pseudo
     CDS             1024..1704
                     /locus_tag="ACHY09_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1721..2029
                     /locus_tag="ACHY09_004162"
                     /pseudo
     CDS             1721..2029
                     /locus_tag="ACHY09_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2132..3238)
                     /locus_tag="ACHY09_004163"
                     /pseudo
     CDS             complement(2132..3238)
                     /locus_tag="ACHY09_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4004..6052
                     /gene="ireA"
                     /locus_tag="ACHY09_004164"
     CDS             4004..6052
                     /gene="ireA"
                     /locus_tag="ACHY09_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFS3887055.1"
                     /translation="MKNKYIIAPGIAVICSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKEDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
     gene            6718..>8401
                     /locus_tag="ACHY09_004165"
     CDS             6718..>8401
                     /locus_tag="ACHY09_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFS3887056.1"
                     /translation="MNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDETDKGYQVYG
                     DNWKSIEIGTTKEVKKKNSTDLNFDHNSPVAKGHNSIAIGTSAEAGGEIKDEKGFKTG
                     ESIAIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGDRFGDKEIPNNYSSS
                     EASGDGSIVVGSRSTAAGDLSVSLGSISSANGTAANALGATAEANGDMATAVGFNALA
                     NASNALAVGSQSSAGGEDSLAIGSQSTTGKKSTVALGQGATASAAEGNVAIGADSVDK
                     AATPVSGATIKLKNGGTIEYRGFAGDKAASVVSVGDAGKERQIVNVGAGAVSEKSTDA
                     INGSQLYAIAKTLKDDLDAINPTWKLKDSTGKEKEVGSKNGGTTGNEVTVTGKGDIDV
                     TVDGNGLSIDGTKLAENIKKDAIKIKTGDGKTLTKKLGEEVEFNDAGPIKTKINNNKL
                     EITAETASFTPDGNGGNGKITVSNGDDDKLVTAKNIADAINQSGWKIKAGSENGGKVE
                     GDNKEELINPGDTVTLKAGKNLSVKQSNGDFTFSVVDTPSFSSVQLNGKGNNG"
BASE COUNT         2455 a         1746 c         1975 g         2225 t
ORIGIN      
        1 atacagtcgc cgtactggtt ccttccctga cagataaaac cagctcccgc tttatgcagt
       61 cgttgcagca ggtgcttaat aaaaatgaat tccagctact gcttgggtgt catgagtata
      121 atcagctcaa agaagctgaa atcctgatga cgcttcttca aggtaacccg gcggcgttag
      181 ttatttttgg ttctcagttg gctgataaaa ctcaccagat actcgagaaa acaaatatac
      241 ctaccattaa cgttgtcggt tcccctttca gtccggctca aattaccatc gaaacagcat
      301 tttttgaagc atcacataaa ctgaccgaac atctgctgga acagggttat aagaatatcg
      361 gatttatcgg tgcgcatatg gacaaccgcc tgcaacgtca gcaactgaac ggctggcata
      421 aagcaatgct gagctattac cagaactctg atcttgttat caccatgccg ggtgccgcaa
      481 gtttgcaatt ggggcgttac gcattaaacg agatattact gcgacaacca gagctggatg
      541 ctgtgatttg cagccacgaa gagattgcgc tgggtatcat gtttgagtgc cagcggcgtt
      601 tgttaaaaat ccctggaaat atcgctgtag cttgtcttga cggttcagac agttgcgatc
      661 aaacacaccc aaccttgaca tccatccgta ttgactacaa aaaaatgggg acagagacgg
      721 gtaagctgct gattggtcta ttgaataata atcatgatga atcagaagag tcgcgtatcg
      781 tgcaattcaa ctaccagata gaattacgtc agagtacatg agctcaaaag gggattattg
      841 caaagttagc tattgtggta gggatagccg tttccggcta ttccccgcgc agagccggat
      901 gtgtgctgct aacgcatcct gtcaacgacg gatgaaaagt tatccactta tatctccacc
      961 aacggcccaa tattgtatcg ttcgtgacac atccatttct acttcaggta tttggcgtag
     1021 tagcgggtgc taaaaatttg ccgcaaaacc gcaatatcag cccgcagagg cgctctccag
     1081 attatccatt cctatattat ctctgcaccg gatgaattac tcgaacaact cagaaatatg
     1141 acacatatgc aacttatcag gactctggga tcctggaggc ctgatgccag tgaatacagt
     1201 aatgttacca acgtttatcg cattacattg aaatcccttg ttcgacgcta tttcgagttt
     1261 catgatgaaa tcaccaactt ggatgtcatg attgcggcaa ttgttgatga actggcacct
     1321 gaactgatta aacgtaatgc tgttggatac gaaagcgctt tgcagttgtt gatctctgcc
     1381 ggagacaatc cctaacggtt aagaaccgaa tcaggttttg cggcgttgcg cggtgtcagc
     1441 cagtttcccc tatcttctgg aaaaacgaac cattaccgac ttaatcgggg tggggagcgt
     1501 gttgcaaata gtgcacttta catcatcact ataggatgtt tacaaactga cgataaaaca
     1561 aaggaatatg tctccaggcg agtagccgaa ggacatacaa aaatggaagc aatacgctgt
     1621 ctgaagcgct atatctcacg cgaagtttat acattactac gaaatcaaaa caggcatgtt
     1681 aactgcatcc cgataacgtc ttgactctta gaagggcgtc caaggcagtc attattcgag
     1741 caggcagttc cggcagttat tgtgtcgata ccagatcagg cagagtatga gccggcgcgg
     1801 aaaatgctgg gataacagct caatggaacg cttcttcagg agtctgaaga acgaatggat
     1861 gccggtggtg ggttacgtaa gcttcagcga ggcagctcac gccataacgg acgatatcgt
     1921 tggatattac agcgcactaa gaccgcacga atataacagt gggttatccc caaacgaatc
     1981 ggaaaatcga tactggaaaa actctaactc ggtggccagt ttttgttgac cacttcagcc
     2041 atcgccagac gctaacatga gttactcttg ttctgggcta cgtcaccttc agggaactgg
     2101 ttatcctcat tctttatttg tgacattgtg gttattgtgc gacaaacatg gctatcccgc
     2161 cccatatccc agataaaacc acctgctcac gtgcgacagc gacaattgta atattgacat
     2221 tctttccttt ggcctgaagt tttcgagacc tcttgcaaag cctgagttga gcatcccatg
     2281 ctctgtcaat gacggggcgg ggtaaatttt cctgattttt tgtattgccg gactgatgcg
     2341 tgtcggatgc cggtaactcc acgctgcctc aaccaatagc tttctggcat agctgtttcc
     2401 ggctttggta atgctgccct gatgtctgct tccacccgat gaatattctg atggcgtgat
     2461 accaaaccat gacatcagtt gtcgtggatg ttcaaaacgg gtcagatcac ccagttcaga
     2521 aattaatcca accgcagtaa taaactgaat ccctcgcatg gcttgctgcg cctctactac
     2581 cggatacagc ctccattcgg taacggcctc cttcgggcgg attccagccg ttcacattgt
     2641 gcctgccggt cttctattgt acaacggtgt tcgtcaaaag ctaattgtcg ccagggactt
     2701 tcgaatgagt atttgctgag ccagtgtcgg tgtgcagggc cccaatctgc tcgcccgaca
     2761 taatggaccc catgaaccag aagaaacgat ttcagacgtt gccttgcatg ccgtaaatca
     2821 tcgcgggcag atgcccatgc ccgggccaaa tcccggaatg tctcatcttc aacgccggat
     2881 acgtagacag cagaaagatc tcctgcacgc agtgaacgca caagtctgat ggcatcacgg
     2941 tgtcggtttt accccgctcc cccggttttt tcgggataag tgagggggcg cagaccatgc
     3001 agtcaaaacc ggatttcacc agccgacgat aaagtccata accacagggt cccgctttat
     3061 aaacgatact gacctgcgat gactttgacc tgatacgttg ataaagatta tgaatgtcag
     3121 taggtgatgt gctaatttta cccatcagtt caacaggttc tgaattgata gcataggaga
     3181 cagtaatcga ttctttgtga acatctaatt cgacgtacaa catgttattg ttgttcatgc
     3241 tggcctccgt ggtggaaatt ccaggggggt agcaacagct cacccatgcg gctctgacaa
     3301 cgatgttaac ccgcattaaa tatcccacgt tggacagcat ctgtctcacg gaaagtcata
     3361 ctgccttacg tgcatatttc accttacgcc ttcactgtct gtcatcggaa gtactgattt
     3421 atattggggc tttgcactta ctgtcattca cgggaaaaga tggtggtgaa cagtacgata
     3481 ctgtcttggc tgttgccgag ggaagattca ggagtggtaa gggggttagt ggtgtaagat
     3541 gagtctggag ctctgactgg tcagtgacgc gaaactcaat ctgccagggt aaactccccc
     3601 ttgcttttgc gcagaagcag tgggccttgg ttcgcttatg tcctgcatct acaagcgaat
     3661 gactgaccgg agactcccat atctggtcag tcgcccattt ttattttcca ccacatcgga
     3721 caagcatacc gtaataaaca gcaccagtct cctgttcact cttgcttgat ttactggaat
     3781 gctgtttatg gagatatccg gttgaaagat tttacctctt cgacaggact ataaattata
     3841 cagtaagaag tccctcgtag aaaggggctg attctcatca agtcggacag gttcaaagtg
     3901 ttacctgaat gagctaaatg ttatttacct tcctttctgt tcattgtaaa tttttcttgc
     3961 taacgcaaat cattatcatt acgattttag aaaagggaaa tttatgaaga acaaatatat
     4021 cattgctccg ggcattgccg tgatatgttc tgcagtcata tcatcaggtt atgccagttc
     4081 tgataaaaaa gaagatacgc ttgttgttac tgcctccggc ttcactcagc aactcagaaa
     4141 tgcaccggcc agtgtttcag tcattacttc agagcaactg caaaaaaaac cggtttcaga
     4201 cctggtcgat gcggtaaaag atgttgaagg gattagtatc accggtggga atgaaaaacc
     4261 ggatatcagt atacgtgggt taagtggcga ttatacattg attctggtcg atggacgacg
     4321 tcagagtggt cgggaatcca gaccaaacgg cagcggcggt tttgaagccg gatttatccc
     4381 tcctgtggaa gcaattgagc gtattgaagt tatacgtggc cctatgtctt ccctgtatgg
     4441 ttccgatgct atcggaggag ttattaatat cataaccaaa cctgttaata accagacatg
     4501 ggatggcgta cttgggcttg ggggaattat tcaggaacat gggaaatttg gtaactcaac
     4561 cacgaatgac ttctacctgt caggcccatt ggttaaggat aaacttggtc ttcagttata
     4621 tggaggaatg aactatcgca aggaagatag tatctctcag ggaacaccag caaaagataa
     4681 taagaatata acggcaacac tccagtttac tccgactgaa agccagaagt ttgtttttga
     4741 atacggaaaa aataaccagg aacatacatt aacacctggt gattctcttg atgcctggac
     4801 tatgcgggga aatcttaaac aaccaaacag taaaagagaa acacataact cacgtagtca
     4861 ctgggtagca gcatggaatg cccagggcga aatactgcat cctgaaattg ctgtttatca
     4921 ggagaaagtt attcgtgagg ttaaatctgg taaagaagat aaatataatc attgggatct
     4981 taattacgag tcaagaaaac cggaaataac caacacgatc atagatgcaa aagtgacggc
     5041 atttctgccg gaaaatgtgc tgaccatcgg aggtcaattt cagcatgcag aactccgtga
     5101 tgactcagcc acaggtaaaa aaacgacaga aacacagtct gtttcagtta aacagaaagc
     5161 tgtttttata gaaaatgaat atgctgcaac ggattctctc gccctgactg gagggctgcg
     5221 cctcgataac catgaaatct atggcagtta ctggaatcca agattgtatg ctgtttataa
     5281 cctgaccgat aatctcactc tcaaaggagg gatcgcaaaa gcatttcggg ccccttcgat
     5341 tcgtgaggtc agtcctggat ttggaacact gacgcagggt ggtgcctcta ttatgtatgg
     5401 aaacagggac ctgaaaccgg agaccagtgt aaccgaagag atcggtatta tttataataa
     5461 tgacagtggt ttttcagcaa gcgcgactct gtttaataca gattttaaaa ataagctgac
     5521 cagttacgat ataggcacaa aagatccagt cactgggcta aacactttta tttatgataa
     5581 tgtgggtgag gcaaatatca gaggtgtgga gcttgcaact cagattcctg tgtatgataa
     5641 atggcgtgta tctgcaaact atacattcac tgactcccgt cgagaaagcg atgacgaaag
     5701 tctcaatggc aagtcgctga aaggggaacc tctggaaaga actcccagac atgcagccaa
     5761 tgcaaaactg gaatgggatt acactcagga tattacattt tattcatctc tgaattatac
     5821 aggaaaacaa atctgggcag cacaaagaaa tggtgccaag gttccccgtg ttcgtaatgg
     5881 attcacttct atggatattg gtatgaatta ccagttcctg ccaggcacgc tgattaattt
     5941 tgccgtactt aacgtcacag acagaaagag cgaagatatc gataccattg atggtaactg
     6001 gcaggtcgat gaaggacgtc gttactgggc taatgtaaga atatccttct gagagaatat
     6061 agataacctg aggccgtgcg cctcaggtac cagtatagta atcagtaacg tcagcttcca
     6121 ggttgtaatg cttttaattt tgataaaaat aaacttaata aattacattt gttaaatatt
     6181 gataatttta atttggtttt attactccag ttatactcac actctatctt tacacaattc
     6241 atcaccatta cacctccata gcctgttgat ttaaaagatt taatttcctg tgaaacaatc
     6301 aggcctgtat gaacctgatt ataccacata atcttttaac ttatttaaat ttaactctca
     6361 ggcttttctg tcagtaaaat cagaaagaat tgcaggattt cgtttttttg ctgcgaagtg
     6421 aaatgtatct catttttact gccttcgcca gtgcgtcagg caatggcagg tagtgtacct
     6481 gcattctgct gtaacgcgta atgtcgggta ttttacttaa tatgttttat gtttgcatta
     6541 ttataaatca ctttattgcc ttttgtctgc tttttaatgg ttcctgtttt tttacttctg
     6601 gctttatata aatggatgct gaagaaatga aggcagtcca ccttacataa tattcatgca
     6661 tgatttttat gctattccga ctgagtggaa gaaaataaag gagaaaagag gatatatatg
     6721 aacctcagaa aaaaacatat ctgtatagcc atatcacttg ttcttaatcc gttccctgca
     6781 tcactggcag cacaaaacac taatgtagtt gaagttgatg aaacagataa aggttaccaa
     6841 gtttatggtg acaactggaa aagtattgaa ataggtacaa cgaaggaagt aaagaaaaag
     6901 aattcaaccg accttaattt tgaccataac tccccggtag cgaaagggca taactccatt
     6961 gcgattggta ccagtgcaga agcaggaggt gaaattaaag acgaaaaagg ttttaagaca
     7021 ggagaatcca tagctattgg tgccaaggcg aaagcaacta aagaacagtc aattgcaata
     7081 ggtggcgata ccaaagctac cggatggggg gctattgtta ttggtggaga tgatttaacc
     7141 cctttgcatg gtgacagatt cggtgataaa gaaattccga ataattactc gtcatctgaa
     7201 gcatcaggag acggaagtat tgttgtgggc tcaaggagca cagctgcagg agacctgtct
     7261 gtgtccctgg gctcaataag ctctgccaac gggacggctg ctaatgcact gggagccacg
     7321 gcggaagcta acggggatat ggcgactgcg gttggtttta atgcgctggc taatgccagt
     7381 aatgctctgg ctgttggtag tcagtcctct gccggtggag aggattccct ggctataggg
     7441 agtcagagta cgacgggcaa aaagtccacg gttgctctgg ggcagggagc tacagcctct
     7501 gccgcggagg ggaacgtggc catcggtgct gattcagtgg acaaggcagc aactccggtt
     7561 tccggcgcta ccattaagtt aaagaatggc gggactattg aatacagggg ctttgccgga
     7621 gataaggctg catcagtagt atctgttggt gatgcgggta aagagcgtca gattgttaac
     7681 gtgggggcgg gtgctgtatc tgagaaaagt acagatgcca ttaacggtag tcagttatat
     7741 gcgatagcga agacactgaa agatgacctt gatgccatta atccgacgtg gaagttaaaa
     7801 gacagcacag gaaaagaaaa agaggtcggc agtaaaaatg gcgggactac aggtaatgaa
     7861 gtgaccgtca ccggtaaagg ggatattgat gttactgttg atggaaatgg gctgagtatt
     7921 gatggcacaa agcttgctga aaacatcaaa aaagatgcca tcaagattaa aacgggagat
     7981 ggtaaaacac taacgaaaaa acttggtgaa gaagttgagt ttaatgatgc cggaccaata
     8041 aaaacaaaaa ttaataataa caaacttgag ataactgcag agacagccag ttttacgcct
     8101 gatggaaatg gtggtaacgg gaagattaca gtaagcaatg gtgatgacga taagcttgtt
     8161 actgcgaaaa atattgctga tgccataaac cagtctggct ggaaaattaa agctggcagt
     8221 gaaaatggtg ggaaagttga gggggataat aaagaggagc taattaatcc cggtgataca
     8281 gttacactga aagccggtaa aaatctcagc gtgaaacaaa gtaacggtga ttttacattc
     8341 tctgttgttg atactccgtc attcagcagt gttcagctga atggaaaagg aaataacgga
     8401 a
//
LOCUS       DAZUCC010000205         8242 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZUCC010000205 DAZUCC010000000
VERSION     DAZUCC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.206"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            280..1095
                     /gene="yedP"
                     /locus_tag="ACHY09_004166"
     CDS             280..1095
                     /gene="yedP"
                     /locus_tag="ACHY09_004166"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFS3887057.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAKQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(1092..2786)
                     /gene="dgcQ"
                     /locus_tag="ACHY09_004167"
     CDS             complement(1092..2786)
                     /gene="dgcQ"
                     /locus_tag="ACHY09_004167"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFS3887058.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     VGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(2957..3139)
                     /gene="yodC"
                     /locus_tag="ACHY09_004168"
     CDS             complement(2957..3139)
                     /gene="yodC"
                     /locus_tag="ACHY09_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFS3887059.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(3218..4135)
                     /gene="yedI"
                     /locus_tag="ACHY09_004169"
     CDS             complement(3218..4135)
                     /gene="yedI"
                     /locus_tag="ACHY09_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFS3887060.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4308..5228
                     /gene="yedA"
                     /locus_tag="ACHY09_004170"
     CDS             4308..5228
                     /gene="yedA"
                     /locus_tag="ACHY09_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFS3887061.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(5217..5687)
                     /gene="vsr"
                     /locus_tag="ACHY09_004171"
     CDS             complement(5217..5687)
                     /gene="vsr"
                     /locus_tag="ACHY09_004171"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFS3887062.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(5668..7086)
                     /gene="dcm"
                     /locus_tag="ACHY09_004172"
     CDS             complement(5668..7086)
                     /gene="dcm"
                     /locus_tag="ACHY09_004172"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFS3887063.1"
                     /translation="MQENISVTDSYSTGNVVQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEVKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(7153..7848)
                     /gene="yedJ"
                     /locus_tag="ACHY09_004173"
     CDS             complement(7153..7848)
                     /gene="yedJ"
                     /locus_tag="ACHY09_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFS3887064.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQHSSILAAEEARRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFSQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(7888..8169)
                     /gene="drpB"
                     /locus_tag="ACHY09_004174"
     CDS             complement(7888..8169)
                     /gene="drpB"
                     /locus_tag="ACHY09_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFS3887065.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         1954 a         2271 c         2027 g         1990 t
ORIGIN      
        1 ccgaccctga gggggtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt
       61 acaccaggaa atctgatgtg ttcatcacct tatccgcaat ttttttcgct gacaagaaaa
      121 tattcgtgat atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct
      181 tggtggctct ggcgtcaacg ggatcgctcc caaaaaaatc atctttgata atttgtgctg
      241 ttttgagaat catcctggta aggggagtac tgcgcaacca tgctttcaat tcaacaacca
      301 ctactggttt ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa
      361 ccggcagccc cctggctcag ccgtttacgc gaagcgaatg ttcccgtcat tctctgtagc
      421 agtaaaactt cagcggaaat gctgtacttg caaaaaatgt tggggctaca aggcttaccg
      481 ctgattgcag agaatggcgc agtgatccag cttgctaagc aatggcagga gatagacggt
      541 tttccacgca tcatctcagg tattagccat ggcgaaatca gccaggtttt aaatacgcta
      601 cgcgagaaag aacattttaa attcacgact tttgatgatg tcgacgatgc aaccatcgcc
      661 gaatggacgg gattaagccg tagccaggcg gcgctgacgc agctgcatga ggcgtcggta
      721 acgctaatct ggcgcgacag tgacgagcgt atggcacaat ttaccgctcg tctgaacgaa
      781 ctgggcttgc agtttatgca gggtgcgcgc ttctggcacg tcctggatgc ctctgccgga
      841 aaagatcagg ctgccaactg gattatcgcg acctatcaac aattgtcagg caaacgccca
      901 accacacttg gcctgggcga tgggccaaac gatgcgccct tactggaggt aatggattac
      961 gcggtgattg tgaaagggct aaaccgtgaa ggggtccatc tgcatgatga ggatccggcc
     1021 cgcgtctggc gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt
     1081 ttctccgccc gttaagcgtt atcgctcgcg aatacccgat tgcgcccggc ctgttttgcg
     1141 aggtaaagcc gacggtcggc caatgactgg agttgttcaa aatcataatc accggtttcc
     1201 tcgctgctgc ttacccccag cgaggcactg atgcgtatcg tcgtactctt ggcgatcagc
     1261 atctcttttt catttaactt caggcgaata cgttctgcga cctccgcagc ctgcgtcaga
     1321 ttcgcgcctg gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacgtcctgc
     1381 gcacgtaagg aactgctaat taatccggca gcatgagaaa gaacacggtc gcccacctga
     1441 tgaccaaagc ggtcattaat cgctttaaaa tggtcaagat cgacctgaat gacagaaaat
     1501 ggatgttggt gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca
     1561 cggttatata aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaga
     1621 acatacatgt tgctgaccat ccggcgaatc acataccagg agagtagtaa catggtggta
     1681 aagagcgccc acagcagggt taatgcaatg ctgatactgc cgaaatcacc acgcacgcct
     1741 tcgcttagcg tatggacgcg caccagcaca ccgtcgaaat ggtccagacg ttcccagcta
     1801 acataacgac tgttcatacg aatgccaccc cgcgtgtcat gttccattgc ctgcgccaac
     1861 aaggccagtt cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc
     1921 aaaaatctca gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg
     1981 tttcttaaaa attgctgcat ggtacgcacg ggaatactca tccccagcac gccataccag
     2041 tagttttcac tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt
     2101 tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg
     2161 ataaaccagg gttgggtgac atagccgtaa taacgcgttg gtacattgcg cgtaaacaag
     2221 gtcggctgcg tcgaaacgta aaatccggca cgcgagacat acatcgcctg ttcaaccatc
     2281 gacgaggagt tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca
     2341 ttgtcgaggc tttcattttc gcgagacagg agattcccct cgctgactaa tgcatccgac
     2401 acgccattga ctggcagggt gcgtcgtcgg ttgagttcga tttgccaggc gtgctcgtcg
     2461 cgatgctgtt cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgctacg
     2521 agagcttcgc gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc
     2581 ttatcgagcg cgttggctac gttttccaga tgattacgct ggctggagat ataggcatct
     2641 tccagcacca ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag
     2701 cagagattaa cgacatgacc aggccccagg cggcgtgcga gttttttcaa ccagctctgg
     2761 ttttccattt ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct
     2821 ggcataaccc ggcgataacg taccgcaaaa cagcgataat tttataatgg tggcatgata
     2881 agacgcgact atcattcatt gcaggcattg gcacaccagg ccggatgtga tccggcctgg
     2941 tgttaatgat accctttcaa acttcttccg cagaacgact cccctccccc ggaacaagat
     3001 cggtttcatg aaaagcttcc cgcttgaccc cgtaaccgtc ataccaacga cactcaacca
     3061 taccgctgga gtatccagtg acaatcatcc gcgggccgcc ctctttaacc gtaacttcct
     3121 cactaaccat aaagctcata ctcgcctcct tttttcgagt gaaacttgtt caccttagtt
     3181 gaagatggcg aattttgcat aatagccagt gcgagtatta atgtgcctga ccgcgcattt
     3241 tcgctactgc tttcactccc agcaccacga tgccgccgat gataaaacca agaatcagat
     3301 ttaaaacagt cggtaatatc atcgccacca ctgcactttg ctgcccggcg aaatgttcaa
     3361 tggcgtgatg cagcggcgca ataccatgta ccacaatccc gccgccgacg aggaacatcg
     3421 ccagcgtgcc gacaatcgat aatgctttca tcagccaggg cgcgataatc aataaccctt
     3481 tacctaatgc ctgcatcagc gcgctggatt tttccgccag ccaatacccc aggtcatcaa
     3541 tcttaacgat aacccctacc agaccgtaga cgcccacggt cactaccagc gcgatgcctg
     3601 aaagcaccag cacctgatta agcaacggcg cctctgccac aatccccagc gtgatggcaa
     3661 cgatttccgc agacaaaata aaatcggtac gaatcgcccc tttaattttg tccttttcaa
     3721 acttcagcgg atcctgcgcc gccagcttct ccagacgctg ctgactctgc gccggatctt
     3781 ctttatgttt acgcgcctcc agcatatgca gcactttctc taccccttca aagcagagaa
     3841 acgcgccgcc aatcatcaac agtggcgtaa tcgcccacgg gataaacgca ctgatgatca
     3901 gcgccagcgg caccagaatc actttattga tcagcgatcc tttcgccacg ccccagacca
     3961 cgggaagttc ccggttggcc cgcacgcctg aaacttgttg cgcattgagc gataagtcat
     4021 cccctaatac accggcggtt ttcttcgccg ccagtttgcc catcacggaa atatcgtcca
     4081 gcagtgtggc gatatcatcg agcaacgtca gtaaactact cccggccaaa agcattccct
     4141 ctcattttta ttgttgaagg gtgtaagtat gaagcaaaaa cggcagctct gatacttgct
     4201 tcacaggtca acattttttt aacaatcaag tgcaattata tctctcgcca gcagaatgat
     4261 tttcacgttt actatgaccc acctttttat ttcagctgtg agggattatg cgtttccgcc
     4321 agttgttacc gctttttggc gcgctgtttg cgttgtatat catttggggc tcaacctatt
     4381 ttgtcattcg gattggcgtg gaaagctggc cgccgttaat gatggcgggc gtgcgattcc
     4441 tggcagccgg tattttattg ctggcatttt tgctactgcg cggacacaaa cttcccccgc
     4501 tacgtccgct gctcaatgcc gcgctgattg gcctgttatt gctggctgtc ggtaatggca
     4561 tggtgacggt tgccgaacat caaaatgttc cttccggcat cgccgccgta gtggttgcaa
     4621 ccgtgcccct ctttaccctg tgcttcagcc gcctgtttgg cattaaaacg cgcaaactgg
     4681 aatgggtggg tatcgccatt gggcttgccg gaatcatcat gctcaatagc ggtggaaatt
     4741 taagcggcaa tccgtggggc gcgattctga ttttaatcgg ctcgattagc tgggcgtttg
     4801 gctcggttta tggctcgcgc attaccttac ctgtagggat gatggcgggt gcgattgaga
     4861 tgcttgcggc aggcgtggtg ttaatgattg cgtcgatgat tgcgggtgaa aaactgacgg
     4921 cgctcccttc cctttcaggc ttccttgcgg tcggctatct ggcgctgttt ggttcgatta
     4981 tcgccattaa cgcttatatg tatttaatcc gcaacgtcag tccggctctc gccaccagct
     5041 acgcctacgt taacccggtg gtcgcggtct tactgggtac gggactgggt ggcgaaacgc
     5101 tgtcgaagat tgaatggctg gcgctcggcg taattgtttt cgcggtggta ctggtcacgt
     5161 tgggaaaata tctcttcccg gccaagcccg ttgtcgcgcc agttattcag gatgcatcaa
     5221 gcgagtaaat gaatcccctg cgtgtcgatc tgcgcgctgg caccttcgcc gcagatccac
     5281 tcttccagac gctcggtaag cgccgcatcc gtaagcttct cgcgcccacg taacgcacac
     5341 tcccagacaa tcagtacgcg ccagccgagt tcctgcaagc gactgatatc gcggcgatcg
     5401 cgttcaacat ttttacctat cttctccagc cagaactcgg ttcgagtcgc aggcacttta
     5461 aacagatagc agtgatgatg atgccagaag cagccgtggg taaatatcac gcagcgatat
     5521 tcatcaacga caaaatccgg gcgtccgggc agactggcgt cctgaacgcg aaacgcaagg
     5581 ccctgcccgg ttaacaaact ggcgaggcgc ttctcaatcg ccgtatcacg cgtggcaatc
     5641 gcgcgcatat ttttgctgcg ggtggcctta tcgtgaacgt cgaccatgtt gtgcctcttg
     5701 ctgacgcaac gccaccgcct gtttgatttt tggctcaagc agttttgcca ccgcggcaaa
     5761 gaccggcacg accaccgagt taccgaactg gcgataggcc tgagtgtccg aaaccggaat
     5821 gcggaatttc acttctcctg gcgcttcaaa acccattaag cgcgcacatt cccgaggcgt
     5881 taaccgacgt gggcgatgtt gctgattcag cggatcgtca aagtcttttt cacccgtggc
     5941 catatcccag ccgcgatcga ttaaaatttc cgcgccatct ttgtaataac gagcagacag
     6001 cgtacgcgtg acgctttgcg gattgttcgg ataaaccatt ccataaccga agccattacc
     6061 gcgcgcctga tgttttttcg cataacgata gaggtacttc cacagcaccg gcgtcaggat
     6121 atatttcgcc tcgaccatcg ggtccagcag ctgggccagc gtcactcgct gcgcagggaa
     6181 acattcgctg atatcacgca gggtaaaatc ggctttcaga ttcagatcgc gacgaaaacc
     6241 caccagcacg atgcgttcac ggtgctgcgg caggaaatgt ttgccatcga tgattttcgg
     6301 atcgtccggc ccgttatctt ctgcatcagc cacgtcatag cccagttcgt ccagcgtctg
     6361 catgatgatg cggaacgttt taccctggtc gtgacttttc aggtttttga cgttttcgag
     6421 cacaaacatc gccggacgac gcgcgtcgat aatgcgcacc acatcaaaga acagcgtgcc
     6481 ctgagtatcg caggcaaaac cgtgcgcccg cccgagcgag ttctttttcg atacgccagc
     6541 cagcgaaaat ggctgacaag gaaaacccgc cagtaataca tcgtgttcag gaatgtgttg
     6601 acgaatatgt tccgccgccg cctcatcact cacgccttct ttatggctga gggtgatgtc
     6661 gcggatatct tcattaaaat gatgcgtcgc cgggtcgcaa taatgattgg ctttataagt
     6721 gcgtaccgca tgtttgttcc attcgctggt aaacacgcac tgtccgccaa tcgattcaaa
     6781 accgcgacgg atgccgccaa ttccggcaaa tagatcgata aagcgaaacg cataatgcgg
     6841 atgatgtgcc ggtggtttgg gtaacaacgt ttgcagatgg gcgaactctt tctcacttaa
     6901 acggtgccat gccgagtcat tcgccagcgc acgttttaaa attgccgcgc tccagtgatt
     6961 ctcacccaca ccattaagct gcgccaccag cgttttaaca tcataaattt gcagcagttt
     7021 ctccagcatt gcctgaacga cattcccggt gctgtatgaa tcggttactg atatattttc
     7081 ctgcatagat ttaaccggcc atctgagatg gccgacaggt taacataatt acagccattt
     7141 agggccacgc gctcagcccg cgggtgaaaa cgcatctatc accttgtgat cgacaccttc
     7201 attctccccc gccagttcgg cactgagctt cgccataaac tcgactaaaa attgcgcgtt
     7261 gtgctgcgcc agctgcttgc ctcttgcggt ttgcatggtt tgcggtagct tcagcaactt
     7321 agtctggaaa tgatccagcg cgtagcgttt atcatccagc gggcgatgct gtgagaacgg
     7381 atcttcacca tcaaacagcg ccacgcccaa tgcccctgaa acagcaaaca cgcgcgccag
     7441 gccaatcgcc cccagggctt ccagccgatc cgcatcctgg acgattttag cttccgttgt
     7501 taagggggcg atttgcgcgc tgaaactgtg agcggcaatg gcatgacaaa cggcctcgat
     7561 tttctccgcc ggaaattgca cgaactcttc acggagcagg cgacgcgcct cttctgccgc
     7621 caggattgaa gaatgctgcc gttgcggatg atttttcgcc aggctcacaa tatcgtgaaa
     7681 ataacaggcg gttaaaatca ccagcatatc gacatcgtca tctgccgcca gcttctgtgc
     7741 cgtagcccag acgcggcgaa aatgacatac gtcatgggcc gcgtcctggt gctgatgatg
     7801 gtttttcaac cagttttcga actgtgcctg ccagtgttgt aagtccatac gcgctccgtt
     7861 gccaaagagt cgcaacctta gcagttttta ttcatagcgt ctgctacgtg cgggacgagg
     7921 tcgggtgtac caggcaatgc tgcctaacaa tgccccgacc agagccataa ataaaaatcc
     7981 gaccagtgcc cctaaccact ttcccgcggt agagcctaaa tcgctttcaa atcccgacac
     8041 cggggcatca gggatccggc tcatcaccca gatgtaacgc gccattgccc agacgaaata
     8101 gccacaccat gtatagaaag cccacaaagc caatttgccg ccagggctgc gagagagtct
     8161 ctcttccatc tttgtcgaac cgtattccat tttgagataa cccggaaaat tcagacttag
     8221 tactattatg tgataaaagt ca
//
LOCUS       DAZUCC010000206         8207 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZUCC010000206 DAZUCC010000000
VERSION     DAZUCC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.207"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(24..662)
                     /locus_tag="ACHY09_004175"
     CDS             complement(24..662)
                     /locus_tag="ACHY09_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3887066.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKAGDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(733..1653)
                     /locus_tag="ACHY09_004176"
     CDS             complement(733..1653)
                     /locus_tag="ACHY09_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3887067.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNSRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSREYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            1791..2690
                     /gene="pgrR"
                     /locus_tag="ACHY09_004177"
     CDS             1791..2690
                     /gene="pgrR"
                     /locus_tag="ACHY09_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFS3887068.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3027..4640
                     /gene="mppA"
                     /locus_tag="ACHY09_004178"
     CDS             3027..4640
                     /gene="mppA"
                     /locus_tag="ACHY09_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFS3887069.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAQTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPVYDKVLAQASTENTVKARNADYNTAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4691..5722)
                     /gene="ynaI"
                     /locus_tag="ACHY09_004179"
     CDS             complement(4691..5722)
                     /gene="ynaI"
                     /locus_tag="ACHY09_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFS3887070.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            5966..6223
                     /locus_tag="ACHY09_004180"
     CDS             5966..6223
                     /locus_tag="ACHY09_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFS3887071.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(6273..7223)
                     /gene="uspE"
                     /locus_tag="ACHY09_004181"
     CDS             complement(6273..7223)
                     /gene="uspE"
                     /locus_tag="ACHY09_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFS3887072.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(7375..8127)
                     /gene="fnr"
                     /locus_tag="ACHY09_004182"
     CDS             complement(7375..8127)
                     /gene="fnr"
                     /locus_tag="ACHY09_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFS3887073.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT         2194 a         1857 c         1909 g         2247 t
ORIGIN      
        1 acgatggtag ctatataatt gatttacatg aagaatggct tatcgccatc agtcccgggt
       61 tgattgattc ctatattctc accaatgtgt ttctcaggtt tatcgatgat atctgtaatt
      121 aatgctgcga cactcttacg tgatacgatc gtacctttga atggttcatt gcgtgaggta
      181 agctcataat caataatatc ttcatcagtc agccaggcag ggcgaagaat tgtatattcc
      241 agtccagaag cctcaattgc atctgcagcc ctgcggaatg gttttagggg ttcaccaata
      301 actgcattat tccattcgcc aaattttcct ggaacttcat cataaatacc gagggaaaga
      361 acaaagatca gacgtttaac atcaccggct ttcatcgcag cgatcactga atttgcctga
      421 atatctaaat cttcccccgt aaggttggca tacacaacat cctgtccctg catggcctgc
      481 ttcagtgctg catgattcag tacatccccc ataataatct tgctattagt gggatagggt
      541 ttgtggattt ttgctggctg acgggcaaat aaagtttgtt taatggtttg tttatctgcc
      601 agttgattaa tcacatggcg ggctatctga ccgccagcac caagaatcag tacatttttc
      661 atgaaacttt tcccagatta ttggcagcaa tatgccaggc catatcacta ttacggattt
      721 gaatatgttt cattacagag tttcctcaaa gaacggagct aatactgaaa ttgcttcagc
      781 cacatagact ttgccgtcat aaagatccat atggtttgcg ccttcaacaa tgtgatagcg
      841 tttatcctgg cttgatgctc gatcgtacag gtcgtcactc atccatttgc tccctgcctg
      901 gctgcccgcc acaatctgta tcggctgagt caggtacact tccgccatat ggtaagcatc
      961 ataggtaata atctggttaa ggctgcgcaa agtagcgtaa cccggtgctg ttggatactg
     1021 cgcgcgaggg gtatggtaat actcccaggc ctgacgcagt tcttcgttcg gcgcatcgga
     1081 ctccttcatt ggtgccagtg gcatagtggc gtattctctg ctgctaatat cgctggttcg
     1141 ggcgtttgaa cccgcgtcaa cgtatggtag ggcatctata gatttcacat tgttttccca
     1201 accattacgg aacatcgaac caatattgac cgcactaaca gtaccgatgg ccttgatgcg
     1261 gcgatcctga attgcagcat tggctgtata tcctgcaccg gcacaaattc ccatcgcacc
     1321 aattcggcta ttgtcgacat aagaaagcgt tgtcaggtaa tcaatcacgg cactgacgtc
     1381 ttcagtacga atgtatgggt tttctaactg acgcggctcg ccgccgcttt caccttgata
     1441 agatgcgtca taagcaatag tgacaaatcc cttttccgcc agtttttcgg cataggttcc
     1501 agccgtttgc tctttaacgc ccccacctgg gtgagatacc acaattgccg gatactgacg
     1561 ggtttcatca aattttggag ggaaatagat cactgcagac aaagagatgg ttggattatt
     1621 gctgttagtg aagctgactt tattattcat cattccgttc ctcatggagt tgtcggttcg
     1681 ttttgacggt tggtgatatc actatagata ttgatcatta agttgattag acccaaaatc
     1741 atgattagac ctatcattta aatgattaat agattgtatg gagatactga atgaagcgtg
     1801 aagaaatcgc tgatctgatg gcgtttgtcg tcgttgcaga ggagcgtagc ttcactcgcg
     1861 cagcagcccg cctgagcatg gcgcagtcag ctttaagcca gatagtgcgc cgtatagaag
     1921 aacgattggg attgcggctt ctgacacgaa ccacgcgcag cgttgtaccc actgaagcgg
     1981 gcgagcatct tttgtctgtt cttggcccta tgttgcatga catagattct gccctgacat
     2041 ccctgagcga tctgcagaac cgcccatccg ggacaatacg tattactact gtagaacatg
     2101 cagcaaaaac gatattgtta ccagctatgc gcacattcct gaaatcgcat cctgaaattg
     2161 atatccagct taccattgat tatggtttga ccgatgtcgt ttctgaacgt tttgatgcag
     2221 gcgtccgtct gggtggggag atggataaag atatgatcgc cattcgaatc gggccagata
     2281 taccaatggc tattgttggc tcaccggatt atttttctcg tcgaagtgct ccaacctcag
     2341 tgtcacaatt aatagatcat caggcaatta atttgtatct tcccacatcg ggtacagcaa
     2401 atcgctggag attaatacgc ggtggacgtg aagttcgtgt tcgcatggaa ggtcagcttt
     2461 tactgaatac gatagacctg atcattgatg ctgcaattga tgggcatgga ttggcgtatc
     2521 taccttatga tcaggttgag cgggctatta aagaaaaaaa actgatacgt gttttggata
     2581 aattcacacc agatttaccc ggttatcacc tgtactatcc acaccgtcga catgctggct
     2641 cggcattctc attatttata gataggctga agtataaagg tgctgtttag cactacttgc
     2701 tgatacatta atttaattct tctcttaacg tattctcagt tcctttcaac gttttggtta
     2761 ttttttattc ttcgtacaat ggcgacagat gctgattatg ataccgaaaa cgggtttgaa
     2821 cgtgcgaagc ccgaacgtag tgttcggagt tctatgtgct ttaccgcatt ttggagacta
     2881 ttatttacac taaatctgat ttgatatatt gatacttaaa acatttgatg cttcctttgt
     2941 cacttttttg atggaagttg tttgcatttc tttaaggcga aacaaataat tacgcatcaa
     3001 ttttaatgtc ggttagaggg aaacttatga agcactctgt ttcagtcacg tgttgtgcgc
     3061 tgttggtcag cagcatttct ctttcgtatg ctgctgaagt tccgagcggc acagtactgg
     3121 cagagaagca ggagctggtg cgccacatta aagatgagcc tgcgtcgctg gatcccgcta
     3181 aagccgtggg cctgccagag attcaggtca ttcgcgatct gtttgaagga ctggtgaatc
     3241 agaacgagaa aggggagatt gtccccggcg ttgcgactca gtggaaaagt aatgacaacc
     3301 gtatctggac ttttaccctg cgcgataacg caaaatgggc ggatggcaca ccggtaacgg
     3361 cgcaagattt tgtctacagc tggcaacgtc tggtggaccc aaaaacattg tcgccatttg
     3421 catggtttgc cgcgctggcg ggaatcaaca acgcacaggc gattattgat ggtaaagccg
     3481 cgcctgacca gcttggcgtc accgcagttg atgcccaaac tttgaaaatc cagcttgata
     3541 aaccgttgcc gtggtttgtg aatttaaccg ctaactttgc cttcttcccg gtgcaaaaag
     3601 ccaacgtaga aagcagtaaa gagtggacga aacccggaaa tctgatcggt aatggcgctt
     3661 atgttcttaa agagcgcgta gtcaatgaaa aactggtcgt ggtaccgaat acccattatt
     3721 gggataacgc caaaacggtg ctgcaaaaag tgaccttcct gccaattaat caggaatccg
     3781 cagccactaa gcgttatctc gcgggggata ttgatatcac cgaatccttc ccgaaaaata
     3841 tgtatcagaa gctgttgaag gatattccgg ggcaggttta tacgccgcca cagctcggga
     3901 cctattatta tgcgtttaac acgcaaaaag ggccgacggc cgatcagcgt gttcgcctgg
     3961 cattaagtat gacgatagat cgccgcctga tgaccgaaaa agtgttggga acgggcgaaa
     4021 agccagcgtg gcattttaca ccagatgtta ctgcaggatt tacgccggaa ccttcgccgt
     4081 ttgaacaaat gagtcaggaa gaactgaatg cgcaggcaaa aactttgttg agcgcagctg
     4141 gttatggtcc gcaaaaaccg ctgaagctga cgcttttgta taacacttca gaaaaccatc
     4201 aaaaaattgc gattgctgta gcatcaatgt ggaaaaagaa ccttggcgta gatgttaaat
     4261 tgcaaaatca ggaatggaaa acctatatcg atagccgtaa caccggcaat tttgatgtta
     4321 tccgagcctc gtgggtaggg gattataatg aaccctccac tttcctgaca ttattaacgt
     4381 caacgcattc aggaaatatt tcacgcttta acaatccggt atacgacaaa gtactggccc
     4441 aggcatcaac ggaaaatacc gttaaagcgc gtaatgccga ttacaatacg gcagaaaaaa
     4501 tcctcatgga gcaagcaccg attgcaccaa tttatcaata taccaatgga cgattaatca
     4561 agccgtggct gaaaggttat cccattaata acccggaaga tgtggcgtac agtcggacta
     4621 tgtatattgt gaagcattga tgtgatggga actggcgtta cccttgtgga taacgccagt
     4681 gatgtctgat ttagcgaccc tggtccggcg gtgtaatgtt atccatatac agcgtctggc
     4741 tggggaaggc aaagtccgcg ccgtgtgact gtacaatatc gataatcttc aaataaacgt
     4801 cttgctgtgc agcaagccat tcagcccata ccgtggtttt ggtaaagcaa taaaccataa
     4861 tattcaatga agagtcagca aactggttga aataaaccag taaggtttgt cgctggtcga
     4921 tggccgggtg atttttcagc atctcacgta cggcttcgac aataacgccc acttttgccg
     4981 catcctcata acgtaaacca atggtcgtgg taatgcgccg gttggtcatt cgtcctgggt
     5041 tttctacgct gatcgacgaa aacagcgagt tcggtacgta caacggacga ttatcaaaag
     5101 tcgtaatttt ggtaattcgc cagccaattt ccgctactgt accttcgata tttctgtccg
     5161 gtgaacggat ccagtcgcca atactaaaag gacggtcgaa atagagcata atcccggaaa
     5221 agaagttact caaaatatct ttaccggcca taccgacagc cagaccacca ataccaccaa
     5281 aggtcaacaa gccagaaagg ctcataccga aatgttcacc ataaagcaga acaagcacca
     5341 caataatggt gattttgatg atacgcgaca taatccgcgc actggtgata tcgcgacctt
     5401 ttttaatctg ctgtttttca aactgattta tcagcagaaa tagcttaatc gtcagaataa
     5461 ccgcaatcag ggacgtacag ataaaatcga taacgcctgg ggtgataaat ttgagtttat
     5521 agttttctat aacataatta atgatgctac caacagcact gataattatg gtgtagatta
     5581 aaaattgcac cgcatggaat aaaaatcctt ttcttttacg atttccgcgg cgaaaccaaa
     5641 agctcatcag aatcaatgct gcgcagctac cgaaaataat gaccagatta agcgcattat
     5701 ttgtaaacag ttcagcgatc attgttttat caggctcctc cagataattg tcgtcatgcc
     5761 ggaaacccct ggcggggcta ttttaccgcg acaattcatt cagatcatca atagtcaggg
     5821 aaggaagtag caacattagc taactaatca atgcgttgat tgtaaatcca gctaagaggt
     5881 gaggttttca gagcagacaa cggtgaaatg tcatggtatt gttacgttta ggtaacaaga
     5941 aatttgtctg cacaaggatt acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa
     6001 gaaatttctc tttggtttgt tggcggtttt cattattgcg gcgtcggttg tgactcgcgc
     6061 gaccatcggc ggcgtgatag aacagtacaa tattccgctg tctgagtgga cgacatcaat
     6121 gtatgtgatt cagtcatcga tgatttttgt ttatagcctg gtctttactg tgttgctggc
     6181 aatcccgttg ggaatttatt tccttggcgg cgaagagcag taagtaaaaa ataggcccga
     6241 taactcgggc cttgtcagtt attgaagagt cgttaatcgt cttcttcgtc atccagttca
     6301 acgggtgtct gatactggtc aggtttaatg accagcaggt cgcagcgaag atgatcaatc
     6361 acctgttccg ccgtgttacc aaggaatgct gctgaaatac cggtgcgtcc gaccgtgccc
     6421 agaaccacaa tccccgcctg taagtgctcc gccaaatcag gaatcacctc ttctggcaga
     6481 cctttttcta cgtgcgtcat gttttcatta atgccgaatt tctgccgcag ggctttcatt
     6541 gccagcaaat gttgcccacg aatggcatcg ttataaacgc tcggatcaaa ttccggcagt
     6601 tcaatcgcga tattaattgg cgttaccgga taagcgccaa ccagatgaac ttcggtatgg
     6661 ttgacttgtt ccgccagttc gatcgtctct ttgaccagtt tttcattgag cgcattatga
     6721 tacggctctt cactggcgag attcaccgcc accagcgcct tacccccttc cggccacggc
     6781 tggtctttca ccatccacac ggggcttggg catttgcgta acagatgcca gtccgttggc
     6841 gtaaaaatca ccgcttccag acggtcatgt tggtgcgcca tttttagcac caaatcgtgc
     6901 ccgccgctga tcacttcctg aatgatggct tcgaaaggac ggttatgcca gaccacttta
     6961 atttcaatgg gaacgccagc attgagataa tattttgcct gctcgtggat ccaggctgta
     7021 cgctggctga tgacgccctg acgcatagcg gtacgttcgt ccggggagag cagggtggtc
     7081 atttcgtatg agaagtcata gatcggcaaa aaggctttaa ttttgccacc aatccgttga
     7141 tgtaaataaa cagctcgccg caatgctggt tggtcgtcct ggttaggatc gataacaacg
     7201 agcatgttct gatacatagc catacagggt ctccttacaa caactgtcaa cgcagtttgt
     7261 aattaaaaga ttaacccata tctggtgaat gaaacagtga tgaaccttct gccagatcaa
     7321 taaatcagaa aaatttaatg atatgacaga aggatagtga gttatgcgga aaaatcaggc
     7381 aacgttacgc gtatgaccag caagctgggc cagcgcatcg ttattttcga tggtgatgta
     7441 tttacctttg actgccagca taccgctttt ctggaagcga cccagcagac ggctgatggt
     7501 ttctaccgtc aggcccagat agttaccaat atcgccacga gtcatcgtca ggcggaattc
     7561 acgaggggag aagccgcgtt gggcaaaacg acgggacagg ttgtagatga atgcagccag
     7621 acgttcctcg gcatttttct tcgacaacag caggatcatg tcctgatcgc ctttgatttc
     7681 accgctcatc agacgcatca tctgctgacg cagattcggc attttaccgg acaaatcgtc
     7741 cagcgtttcg aacgggattt cacataccat cgaggtttcc agcgcctgcg cgaagctcgg
     7801 gtgatggccg ctgccgatgg cgtcaaatcc caccaggtcg cctgctaaat ggaaaccagt
     7861 gatttgctcg tcgccttgct cagtgatggt ataactttta atcgtaccgg agcggatggc
     7921 ataaagcgat ttaagttcat caccagcctt aaacagcgtc tggcctttct gaataggctt
     7981 cttccgctca atgatattat caagctgatc aagctcatgt tcgttgagtg tgaacgggat
     8041 gcaaagctgg ctgatgctgc aatcctggca atggatagca caaccgccag actgaatgcg
     8101 ccgtataatt cgcttttccg ggatcatagg tctgctcaag ccgtaattga tatttgtcaa
     8161 ttttaacatc tttttaggga gcaagtaagt ctaagcaaac cttaaca
//
LOCUS       DAZUCC010000207         8177 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZUCC010000207 DAZUCC010000000
VERSION     DAZUCC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.208"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2896
                     /gene="evgS"
                     /locus_tag="ACHY09_004183"
     CDS             <1..2896
                     /gene="evgS"
                     /locus_tag="ACHY09_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFS3887074.1"
                     /translation="YNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWLD
                     TGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNII
                     TLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEAFITTPYVFV
                     MQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELDA
                     LVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPPS
                     EVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKVI
                     QGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENSE
                     SPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYIC
                     GWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGSG
                     LSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSF
                     GAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLGH
                     IDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQ
                     GDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRLL
                     LKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSSL
                     PIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDIE
                     ALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANILN
                     LQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(2952..4097)
                     /gene="yfdE"
                     /locus_tag="ACHY09_004184"
     CDS             complement(2952..4097)
                     /gene="yfdE"
                     /locus_tag="ACHY09_004184"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFS3887075.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4171..5115)
                     /gene="yfdV"
                     /locus_tag="ACHY09_004185"
     CDS             complement(4171..5115)
                     /gene="yfdV"
                     /locus_tag="ACHY09_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFS3887076.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5185..6879)
                     /gene="oxc"
                     /locus_tag="ACHY09_004186"
     CDS             complement(5185..6879)
                     /gene="oxc"
                     /locus_tag="ACHY09_004186"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFS3887077.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNGIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6933..>8177)
                     /gene="frc"
                     /locus_tag="ACHY09_004187"
     CDS             complement(6933..>8177)
                     /gene="frc"
                     /locus_tag="ACHY09_004187"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFS3887078.1"
                     /translation="TPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTR
                     HQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDH
                     MGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLV
                     SAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDK
                     LGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWE
                     NTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCA
                     PVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTA
                     AVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2371 a         1920 c         1714 g         2172 t
ORIGIN      
        1 ttataactca ccgcgtcaat ataacttttt attaaccaga aaagattcta tcgttcttaa
       61 tgaagtactc aatcgatttg ttgatgcttt aacaaatgaa gttcgctatg aagtatcaca
      121 aaattggctt gatacaggaa atctggcctt tctgaacaaa ccattagaac tcactgaaca
      181 tgaaaaacag tggattaagc agcatcccga tttaaaggtg ctagaaaatc cttactcgcc
      241 accctattct atgacagatg aaactggctc agttcggggc gtgatgggtg atattcttaa
      301 tattattacc ttgcaaacag gtttaaattt ttctccgatc accgtttcac acaatattca
      361 tgctgggaca cagcttaacc ccggtggatg ggatatattg cccgccgcta tttatagtga
      421 agatcgagaa aataatgttt catttgctga agccttcata acaacgcctt acgtttttgt
      481 catgcaaaaa gcgcctgaca gtgaacaaac attaaaaaaa ggaatgaaag ttgccattcc
      541 ctattattat gagcttcatt cgcaattaaa agagatgtat ccggaggtag agtggataaa
      601 agtcgataac gccagcgctg catttcacaa ggttaaagaa ggtgaacttg atgctctggt
      661 cgcgacacag ttaaattcgc gttacatgat cgaccattac tatcctaatg aactttatca
      721 ttttcttatt cccggcgttc agaatgcatc actttcgttc gcttttcctc gcggagaacc
      781 ggaacttaag gatattatta ataaagcact gaatgcaatt cccccaagcg aagttctgcg
      841 cctgaccgaa aaatggatta aaatgcccaa tgtgaccatt gacacatggg acctctatag
      901 cgagcaattt tatattgtta cgacattatc cgttttatta gttggcagta gccttttatg
      961 gggattctac ctgttacgct cagttcgtcg tcgtaaagtt attcagggtg atttagaaaa
     1021 ccaaatatca ttccggaaag cgctctcgga ttccttaccg aatccaactt atgttgtaaa
     1081 ctggcaaggt aatgtcatta gtcacaatag tgcatttgaa cattatttca ctgctgatta
     1141 ctataaaaat gcaatgttac cattagaaaa cagtgaatcc ccctttaaag atgttttttc
     1201 taatacgcat gaagtcacag cagaaacgaa agaaaaccga acaatataca cacaggtatt
     1261 tgaaattgat aatggcatcg agaaaagatg cattaatcac tggcatacct tatgtaatct
     1321 gccagcaagc gaacatgctg tttatatttg tggttggcaa gatattaccg agacgcgtga
     1381 tttaattcat gcactcgaag tagagagaaa taaagcgatc aatgcaactg tcgcaaaaag
     1441 ccagtttctg gcaacaatga gtcacgaaat aagaacacca ataagctcca ttatgggctt
     1501 cctggaactt ctgtcgggtt ctggtcttag caaggaacaa cgggtggagg cgatttcact
     1561 tgcctacgcc accggacaat cactcctcgg cttaattggt gaaatccttg atgtcgacaa
     1621 aattgaatcg ggtaactatc aacttcaacc acaatgggtc gatatcccta ctttagtcca
     1681 gaacacttgt cactctttcg gtgcgattgc tgcaagcaaa tcgatcgcat taagttgcag
     1741 cagtacgctt cctgaacgct acctggttaa gatcgaccct caggcgttta agcaggtctt
     1801 atctaattta ctgagtaatg ctctcaaatt tactaccgag ggggcagtaa aaattacgac
     1861 ctccctgggt cacattgatg acaaccacgc tgttatcaaa atgacgatta tggattctgg
     1921 aagtggatta tcgcaggaag aacaacaaca actgtttaaa cgctatagcc aaacaagtgc
     1981 aggtcgtcag caaacaggtt ctggtttagg cttaatgatc tgcaaagaat taattaaaaa
     2041 catgcagggc gatttgtcat tagaaagtca tccaggcata ggaacgatat ttacgatcac
     2101 aatcccggta gaaattaccc aacaagtggc ggctgtcgag gcaaaagcag aacaacctat
     2161 aacactacct gaaaagttga gcatattaat cgcggatgat catccgacca acaggctatt
     2221 actcaaacgc cagctaaatc tattaggata tgatgttgat gaagccactg atggtgtgca
     2281 agcgctacac aaagtcagta tgcaacatta tgatctgctt attactgacg ttaatatgcc
     2341 gaatatggat ggttttgagt tgactcgcaa actccgagag caaaattctt ccttacccat
     2401 ctgggggctt acagccaacg cacaggctaa cgaacgtgaa aaagggttaa attgcggcat
     2461 gaacttatgt ttgttcaaac cgttgactct ggatgtactg aaaacacatt taagtcagtt
     2521 acaccaggtt gcgcatattg cacctcagta tcgccacctt gatatcgagg ccctgaagaa
     2581 taatacggcg aatgatctac aactgatgca ggagattctc atgactttcc agcatgaaac
     2641 gcataaagat ctacccgctg cgtttcatgc actagaagct ggcgataaca gaactttcca
     2701 tcagtgtatt catcgcatcc acggtgcggc taacatcctg aatttgcaaa agttgattaa
     2761 tattagccac cagttagaaa taacacccgt ttcagatgac agtaagcctg aaattcttca
     2821 gttgctgaac tctgtaaaag aacacattgc agagctggac caggagatta ctgttttctg
     2881 tcagcaaaat gactaaatag cggctcccac aatgttcaaa tgtgggagct atttaccagc
     2941 acatcttaca tttatgatga gaactcctgg cgaatttgtt ccccatgctg gtcgagcgtt
     3001 gccgctcccg gcataacatg cgggtccgcg cagccgctga tttttatcgg attccccggc
     3061 atcattattc ccccggcttc aatcaacata tttctcgcct gagtttgtgg caaattaatg
     3121 gcctcagcca cacttaataa cggcgcgacg ggtacaccaa cttcatgtat tctggctaac
     3181 caaacttcag ctgcctgcgt ttttaacgtc cgctcaatat attgtttaag aatagcctgg
     3241 ttttgtacgc gtaaaatatt gctgctaaat ctgggatcat taaccagttc cgtaagctcc
     3301 agtgcctggc ataacgcaga aaaaagcttg tcattaccac aacaaatcgt aatcggctta
     3361 tcctgagtat cgaaaacatc aaaaggtgcc atgtagggat ggcgatttcc caggcgttgt
     3421 ggtgacttcc ctgtcgcaat atatgccatc agaccatgct ccagaaaact cagcgtggca
     3481 tcaaacatcg ctatatcgac atgcgcccct ctttggctct tttcgcggcc ataaagtgca
     3541 ctcactattc cgctgaataa atagacaccg ccgcatagat ccgcaagaga ggtacccacg
     3601 cgcactggcg gagcatcagg gtatcctgtt tccatcatta tcccgctcat tgcctgaatg
     3661 atggtatcgt aggcaggagc atcttttagc ggaccggtat gtccgaaacc tgacgatgaa
     3721 gcatatataa ggcgcgggtt gatttcttgt aacctttccc atgaaaaccc gagtttttcc
     3781 attgtacctg ggcgaaaatt ctcagctaat acatccgctt gtttaagcat atttataaat
     3841 atacttttat cgtgatcatt ctttaaatca agaaccacac tctctttgcc atgattaata
     3901 aaactgtaat agagtgactg tccatccaca tagggaccaa atgtgcgggt atcatcacca
     3961 tgacccggcg gctcaacttt aattaccctt gcgcccatat tacaaagaag ttgagttccg
     4021 aaaggcccat taaggacatg cgtcatatcg ataactaata agccttcaaa cggccctttg
     4081 cattcattat ttgtcatttg cttatgcctt gaaaaaagcc gaaggtgaaa agacacttcg
     4141 gcatttatgt aaaatcaata acatgattaa ttatgaaacc agtcgactga cataaatcca
     4201 caaaggagcc gtgacgacaa aacccagtac gctcacagcc aatgacgctg taccagtgcg
     4261 ggtgtagaca ttaaaccggc tggcaataat gattccggag aatgccggcg gtaaggcgcc
     4321 tgccagtacc atcatctgca aatgttcgct gttcaaatga catgccatac caacgagaag
     4381 cagcgccagt ggcatcagaa tcagcttcag gaaggtgtta taagcaattt cggcactgaa
     4441 ctcgaattta tgtgcagcca gagtcaaccc cgcagcgaat accgctaccc ctgagttagc
     4501 cttcgcaatc agattaaagg ttgggtccca tgctgccgga atttttaccc caaccaacac
     4561 caggatcgtt gccagaacag gtgcccatac tactggctcc tttgccgcag aaattaatgc
     4621 gctcagatta ctattcttct taccatccgc tcctgaggaa ggattcagca aatacagacc
     4681 aataggaata gtaattgcat taacaataat agaaataatt gctaccacta aacctgttga
     4741 tacggaatca ccataaatag gatcgagaac tgcaaatccc aggaatccaa tggtaggtga
     4801 acctgcaatt aatgcacata ctgctgcttc tgcatgagta cgtttaaaaa atttgtagca
     4861 accgaaccag gagaaaaaga aacatccgac aataaccaca agtgatacaa gggtcagacg
     4921 agtgtccgca aaaatcattt ccctgtttgc ccgggtaata gatacaaata gagctgcagg
     4981 aagagcgtag tttaatacca gtttattaaa tgcccgggct tgatcttctg aaaaagtttc
     5041 tcgtctgccg ctaaaataac ccaacagcat gattacgata ataggcaata aatcgccaat
     5101 aaaaaatgtt agcataatag tatttccgat aagggtcccc tcaccatcgc ataatgagtt
     5161 ggcaagagga gacgatgtca gtatttaatt accagcgact tgttttgggt taagtttggt
     5221 aatatggcca ctttcagttc ctgctgcagg gtcgatgacc acattaataa tggtcggttt
     5281 gcgcgactga ataccggtgg ttaaagcatg acgaagttca tctgtcgtag tgacgttata
     5341 gccaacgcca cgaaacgcat ccattaattt gtcatacctt gcatggtgca gcagatccgt
     5401 tggtgatggt gcaccagcgc cgctgagatc aacaccgtct cctctgtaga tgccgccatt
     5461 attaaaaata acgatcgtca ccggcaggtt atatcgacaa atcgtttcaa tttccatccc
     5521 actgaaacca aaagcactat caccttcaat ggcaacaacc ggagaaccag aggtcacgct
     5581 agcaccgatg gcatagccca taccgatgcc catgacaccc caggtgccac aatccagacg
     5641 acgacgtggt ttatacatat caataatatt tcgtgcatta tccagggtat ttgccccttc
     5701 attaactaaa taaatatcct ggttctcgcg caatacgtcg cgcacagcac ttaatgcatt
     5761 aaagtaattt aatggttgag tatctgtgct taacttttca tgcatttttt gtgcattttg
     5821 ctgcttgtgg atatttaaaa tatcgcgcca taccagtgga gtcgtaaatg tgttttgttt
     5881 cagttctgcc agcatacctt gcatactgga tgcaatatcg ccaacgactg gcacagcaat
     5941 ggggcggttg ctgtcaattt cctgcggttc aatatccagt tgaataaact gtgtatctgc
     6001 cgcccatcct tttttaccgt gtgccagtaa ccaattcagt cgtgcaccaa caagcatgac
     6061 aacgtcagca tttgccaggg caaacgaacg cgcagccgcc gcagaaagtg gatgcgtatc
     6121 ttcgaggatc cctttcgcca tagacattgg caggaatgga atctgggcac tttcaataaa
     6181 ttcacgaagc tgttcatccg cttgtgaata cgccgcgcct ttgccaagga taactaatgg
     6241 tcgttcagct tttgctaata gcgaaattgc gctagcgact gacttcgggc atggcaataa
     6301 tgctggcgac ggattttcaa cttttacaat cgtggttaac gcttcgtctt tttccatcgt
     6361 cgcggccagg acatttgctg gcaaatcaag ataaactccg ccagggcgac ccgatactga
     6421 gacccggata gcgcgtgcca atgcaatgcc aagatcctgc ggctgattaa cgcggaatgc
     6481 tgctttggca tacggttttg ccgcattcat ttggtccagc tcttcataat caccttgctg
     6541 taggtcgacg atcgcgcggt cgctggagcc gctaatcatg atcatcggaa aaccatttac
     6601 cgttgcgttg gccaatgcgg tcaaaccatt gaggaatcct ggcgcagaaa ctgtcaggca
     6661 gatccccggt ttttgggtaa gaaaaccgct tgccgcagcg gcatagcctg ccgactgctc
     6721 atgacgaaaa ccaatataac gaatgccttc cgcctgggca tggcgcgcca tatccgtcac
     6781 aggaatacct acaacaccat aaatagtgtc aataccattc tgttttaatg cttcaacgat
     6841 gatatgcata ccatctgtca tttgaagttg atctgacatg attaataacc tcgaaatttt
     6901 tataaattgg gagccagaag cccccgttaa tatcagatgg cgtggttttg cttcattgca
     6961 gcaatttcat cgtcgctata acccagctcc tgcaatacag cagcggtatg ctcacctaat
     7021 agcggcgcag ctttaatatc cggcgtaaag gcagagaatt tcattggaca accaacggtc
     7081 agatattttc cacgcaacgg ttgttccact tcgacaacac taccactttg gcgcaaagag
     7141 ggatcaagtg aaatttcttt catacttaaa accggtgcac aaggaatatc aaactgagtc
     7201 aaataggcca ccgcttcatg tttatcaata gtgacagtgt atttttcgat ttcagcaaaa
     7261 atatcgaaaa tatgtggctg tcgggcatgg gctgtactgt atgccggatc ggtaatccat
     7321 tctggtttgc cgatggcttt acaggtgttt tcccagtttt gctcctgaat agtgaaataa
     7381 atataggcgt taggatcggt ttcccagcct ttacatttca ggatccagcc aggctgaccg
     7441 ccaccacccg cattaccacc gcggggaact gcatcaccaa atgtgccatt cggatactgc
     7501 gggtattctt ccagataccc caacttatcg agacgctgct ggtcgcgtaa tttcacgcgg
     7561 caaaggttca acacggcatc ctgcattgac atggtgactc gttgcccacg ccccgttttt
     7621 tcgcgatgca gcaaagcagc aagtaaaccg atcagcaaat gcattccggt gttgctgtca
     7681 cccaacgctg cagcgcttac cagcggcgga ccgtcccaaa aacctgtagt agatgccgcg
     7741 ccacccgctg cctgagcaac gttttcatag gcttttacat tcacataagg cgaacactca
     7801 tcaaaccctt tgatcgaacc aaaaatcaga cgtggattga tttcttgaat atgctcccag
     7861 gtgaagccca tatgatcaat ggcccctgga tgaaagttct cgactaagat atcagcttcg
     7921 cggatcagct tttccattac ctctttgcct tccgctgttt tggtatttaa ttcaatagaa
     7981 cgtttgttac tgttaagcat ggtgaagtaa agcgcatcga tatcaggaat atctcgcagc
     8041 tggtggcgcg ttacgtcacc aacgccggga cgttcaattt taatgacgtc agcgccaaac
     8101 caggccagca tctgagtaca agatgggcca gattgcacac cagtgaaatc gagaacttta
     8161 attccttgaa gtggagt
//
LOCUS       DAZUCC010000208         8120 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZUCC010000208 DAZUCC010000000
VERSION     DAZUCC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.209"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..354
                     /gene="ecpR"
                     /locus_tag="ACHY09_004188"
                     /gene_synonym="matA"
     CDS             <1..354
                     /gene="ecpR"
                     /locus_tag="ACHY09_004188"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFS3887079.1"
                     /translation="EWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAGLSRDI
                     RKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTVYTHRR
                     NAEAKLYSKLYKLVQ"
     gene            428..1015
                     /gene="ecpA"
                     /locus_tag="ACHY09_004189"
                     /gene_synonym="matB"
     CDS             428..1015
                     /gene="ecpA"
                     /locus_tag="ACHY09_004189"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFS3887080.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1072..1740
                     /gene="ecpB"
                     /locus_tag="ACHY09_004190"
                     /gene_synonym="yagY"
     CDS             1072..1740
                     /gene="ecpB"
                     /locus_tag="ACHY09_004190"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFS3887081.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1766..4291
                     /gene="ecpC"
                     /locus_tag="ACHY09_004191"
                     /gene_synonym="yagX"
     CDS             1766..4291
                     /gene="ecpC"
                     /locus_tag="ACHY09_004191"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFS3887082.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4281..5924
                     /gene="ecpD"
                     /locus_tag="ACHY09_004192"
     CDS             4281..5924
                     /gene="ecpD"
                     /locus_tag="ACHY09_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFS3887083.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5893..6603
                     /gene="ecpE"
                     /locus_tag="ACHY09_004193"
     CDS             5893..6603
                     /gene="ecpE"
                     /locus_tag="ACHY09_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFS3887084.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQTGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6916..7245
                     /gene="ykgJ"
                     /locus_tag="ACHY09_004194"
     CDS             6916..7245
                     /gene="ykgJ"
                     /locus_tag="ACHY09_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFS3887085.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7257..7423)
                     /locus_tag="ACHY09_004195"
                     /pseudo
     CDS             complement(7257..7423)
                     /locus_tag="ACHY09_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7494..8108)
                     /gene="yagU"
                     /locus_tag="ACHY09_004196"
     CDS             complement(7494..8108)
                     /gene="yagU"
                     /locus_tag="ACHY09_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFS3887086.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
BASE COUNT         2132 a         2102 c         2079 g         1807 t
ORIGIN      
        1 gagtggttat ccgcggtaaa ggggaaacag gtcgtattga ttgcggccag aaagtcagaa
       61 gccttagcaa attattggta ttacaacagc aatattaggg gcgtggtata cgctggactg
      121 agtcgtgata ttagaaaaga actggcctat gtgattaatg gcaggttcct gagaaaagat
      181 attaagaaag ataaaatcac tgaccgggaa atggaaatta tccgcatgac ggctcaggga
      241 atgctgccta aatcgattgc cagaattgaa aattgtagtg tgaagacagt gtatacccat
      301 cggcggaatg cagaggccaa gctgtactca aaattatata agttggttca gtaaactcca
      361 ggcaagttag ttttaaaaat gactcaccgg gacttcatgt cctcaattca actcgggaag
      421 aaaagcaatg aaaaaaaagg ttctggcaat agctctggta acggtgttta ccggtacagg
      481 tgtagcgcag gctgctgacg taacagctca ggctgtagcg acctggtcag caacagccaa
      541 aaaagacacc accagtaagc tggttgtgac gccactcggt agcctggcgt tccagtatgc
      601 cgaaggcatt aaaggtttta actcacaaaa aggtctattt gacgtggcta tcgagggtga
      661 ctcaacggct accgccttta aactgacctc acgccttatc accaacacct taacccagtt
      721 ggatacctca ggttccacac tgaatgtggg cgtggattat aacggcgcgg cagtcgaaaa
      781 aactggcgat accgtgatga tcgataccgc caacggcgta ctgggcggca accttagccc
      841 actggctaac ggttacaatg ccagcaatcg taccaccgca caggatggtt tcactttctc
      901 catcatcagc ggcaccacca atggtaccac cgcagtaacc gattacagca ctctaccgga
      961 aggcatctgg agcggcgacg ttagcgtaca gttcgacgct acctggacca gttaatctct
     1021 ctgatgtacc agcaggggta cccccctgct ttattcctgg atggaattgt tatgaaaaag
     1081 caccttctgc ctctcgctct gctgttttcc ggaatatcac cggcccaggc gctggatgtc
     1141 ggcgatatat catcgtttat gaacagtgac agcagcacgc taagcaaaac gatccaaaac
     1201 agtaccgaca gtggccgcct catcaatatc cgtctcgaac ggctctcttc accgcttgac
     1261 gacgggcagg ttatcgcaat ggacaagccg gatgagttgc tactcactcc cgccagcttg
     1321 ctgctacccg cccaagccag cgaagtgatc cgcttcttct ataagggacc agcagatgaa
     1381 aaagagcgct actaccgcat tgtctggttt gatcaggccc tcagtgatgc gcagcgcgat
     1441 aatgccaacc gcagcgctgt ggccactgct tccgcccgca tcggcaccat tctggtcgtc
     1501 gcaccccgcc aggcaaacta ccactttcag tacgccaacg gctccctgac aaatacagga
     1561 aatgcgacgc tgcggatcct cgcctacggc ccctgcctga aagccgccaa tggtaaggag
     1621 tgtaaagaga attactacct gatgccgggc aagtcgcgtc gttttacccg cgtggacacg
     1681 gcggataaca aaggacgggt tgcactttgg cagggtgata agttcattcc cgtgaaatag
     1741 atagctgtgc agatggataa cgacaatgcc tttacgacgg ttctccccag gactgaaagc
     1801 ccagtttgcc ttcggcatgg tctttttgtt cgttcagccc gatgccagcg ctgctgacat
     1861 aagtgcgcag caaataggtg gggtgattat tccgcaggcc ttcagtcagg cgctccagga
     1921 cggcatgagc gtcccgcttt atattcatct cgccggtagc cagggtcgcc aggacgatca
     1981 gcgaatcggc agcgctttta tctggttgga tgatggacag ttacgcatcc gaaaaataca
     2041 gctggaagag agtgaagata acgccagtgt cagcgaacaa actcgacagc agctgatgac
     2101 tctggccaac gccccgttca atgaggccct taccatcccc ctgactgaca acgcgcagtt
     2161 ggatctcagc ttgcgccaac tgctgctgca gctggtggtc aagcgcgaag cgctgggcac
     2221 cgtactacgc tcacgtagcg aagacatcgg gcagtccagt gttaacaccc tcagcagtaa
     2281 tctgagctat aacttcggcg tctataacaa ccagttgcgt aacggcggga gcaacacctc
     2341 cagctatctg tcgctgaata acgttactgc gctgcgcgaa catcacgtgg tgctcgacgg
     2401 ctcactgtac gggattggta gcggtcaaca ggacagtgaa ttatataaag cgatgtatga
     2461 acgcgatttt gctggtcacc gatttgccgg tggaatgctc gacacctgga acttgcagtc
     2521 cttagggccg atgaccgcca tttcagcagg gaagatttac ggcctttcct ggggaaacca
     2581 ggccagctcc accatcttcg acagcagcca gtcggccacg ccagtgatcg cctttttacc
     2641 ggcggcgggc gaagtacatc tcacccgtga tgggcggcta ctaagcgttc agaacttcac
     2701 catgggcaat catgaagtgg atacccgggg tctaccgtac ggtatttacg atgtggaagt
     2761 tgaggtaatc gttaacggtc gcgtgatcag caaacgcacc cagcgggtca ataagctgtt
     2821 tagccgggga cgtggcgtcg gtgcaccgct ggcgtggcag atatggggcg gtagctttca
     2881 tatggatcgc tggtcggaaa acgggaaaaa gacgcgacca gccaaagaga gttggctggc
     2941 aggtgcctcg acctccggct cactgagtac gtttagctgg gcggcaacgg gatatggata
     3001 cgataatcag gcggtgggtg aaacccgtct gacgctgccg cttggggggg cgatcaacgt
     3061 taacctgcaa aacatgctgg ccagtgacag ctcatggagc aacatcgcca gcatcagcgc
     3121 cactctacct ggaggcttca gttcgctgtg ggttaaccag gaaaaaaccc gcattggcaa
     3181 tcaattgcga cgtagcgatg ccgacaaccg tgcaatcggc ggcacactca acctgaactc
     3241 actgtggtcg aagctgggta cgttcagcat cagctacaat gatgaccgcc gctacaacag
     3301 ccattattac acggcagatt actatcaaag tgtctacagc ggtacctttg gttcgcttgg
     3361 cctgcgggcc ggtattcagc gctataacaa cggcgacagc agcgccaata cagggaaata
     3421 tatcgctctc gatctctcgc taccactggg caactggttt agcgcaggga tgacccatca
     3481 aaacggctac accatggcaa acctgtcagc acgcaaacag tttgatgaag gaaccattcg
     3541 cactgttggt gccaatctgt cacgagccat ctccggcgat accggtgatg acaaaaccct
     3601 cagcggtggg gcgtatgcac agttcgacgc tcgttacgcc agcggaacgc tgaacgtcaa
     3661 tagcgcggcg gacggctaca tcaatactaa tttgaccgcc aacggcagcg tcggctggca
     3721 gggtaaaaac atcgctgcca gcgggcggac cgatggcaac gctggggtga tattcgacac
     3781 tggcctggag aacgatggtc agatcagcgc caaaatcaac gggcggattt tcccgcttaa
     3841 cggcaagcgt aactatctcc cgctctctcc ctatggaaga tatgaggtgg agttgcagaa
     3901 cagcaaaaac tcactcgaca gttacgatat cgtcagcggt cgcaaaagtc atctgactct
     3961 ctatccaggc aatgtcgctg tcattgagcc agaggtgaag cagatggtta ccgtctccgg
     4021 tcgtatccgt gcggaagacg gcacactgct ggctaacgca cggattaaca accatatcgg
     4081 ccgaacccga accgatgaaa acggcgagtt tgtcatggac gtggataaga aataccccac
     4141 tatcgatttt cgctacagtg gcaataaaac ctgcgaagtg gcactggaac tcaaccaggc
     4201 gcgcggtgcc gtctgggtcg gtgatgtggt ctgcagcggc ctctcatcgt gggcggcggt
     4261 gacgcagaca ggagaagaga atgagagtta acctactgat agcgatgata atctttgcgc
     4321 taatctggcc tgcaaccgcg ctcagagcgg cagtgagcaa aacaacctgg gcggatgctc
     4381 cggcacgcga gtttgtgttt gtcgaaaaca actcagacga caactttttc gtcactcctg
     4441 gcggggcgct ggatccgcgc ctgaccggtg ccaaccgctg gaccggttta aaatacaatg
     4501 gttcaggaac catctatcag caaagcctcg gctacattga taacggttac aacaccggcc
     4561 tttataccaa ctggaagttt gatatgtggc tggaaaattc accagtttca tctcctttaa
     4621 ctggcttgcg ctgcatcaac tggtacgcag ggtgtaatat gaccaccagt cttatcctgc
     4681 cgcaaaccac cgacgccagt ggattttatg gcgcgacggt gaccagcggc ggcgcgaaat
     4741 ggatgcacgg catgttgtca gacgcgtttt accagtatct gcaacaaatg cccgtcggca
     4801 gcagctttac aatgaccatc aatgcctgcc agacctctgt gaactatgac gccagcagcg
     4861 gcgcacgctg taaggatcag gcctccggca actggtatgt tcgcaacgtc acccatacga
     4921 aagcagcaaa tctgcggttg ataaataccc actcgctggc ggaagtattt atcaacagcg
     4981 acggagtacc gactctgggc gaagggaacg ccgactgccg gacgcaaacc atcggcagcc
     5041 gttcaggatt aagttgtaag atggttaact ataccctgca aacaaacgga ctcagcaaca
     5101 cctcaatcca tatattcccg gcgatcgcca actcgtcgtt agcctcggcc gtcggggcgt
     5161 acgatatgca gttcagtctg aatggcagtt catggaaacc ggtgagcaat actgcctatt
     5221 actacacctt caacgagatg aagagcgcag actcgatcta tgttttcttc tcgagcaact
     5281 tctttaaaca gatggtgaac ctcgggatca gcgatatcaa caccaaagat ctattcaact
     5341 ttcgctttca gaacaccaca tcaccggagt ctggctggta tgaattttct acctccaaca
     5401 cgctgattat caaaccccgt gatttcagca tcagtattat ctccgatgaa tatactcaga
     5461 caccgtcgcg ggagggatat gttggcagcg gcgagtcggc actcgatttc ggctatatcg
     5521 taaccaccag cggtaaaaca gctgccgacg aagtgctgat caaggtgacc ggacccgcgc
     5581 aggtgattgg cgggcgctcc tattgtgtct tcagctccga tgacggtaag gcgaaagtac
     5641 cgttcccggc gacgctttcc tttattaccc gcaacggagc tacaaaaacc tacgatgccg
     5701 ggtgcgatga tagctggcgg gatatgaccg atgcgctgtg gttgaccaca ccgtggactg
     5761 atatctctgg cgaagtgggg cagatggata agaccacagt caaattttcg attccaatgg
     5821 ataacgccat ttctctgcgt acggtagatg ataacggctg gtttggcgaa gtcagcgctt
     5881 caggagagat tcatgttcag gcgacgtggc gtaacattaa ctaaggccct gctgacagtg
     5941 gtctgtatgc tggcggcacc tttgacacag gcgatttcgg tcggcaatct gactttttca
     6001 ctgccgtccg agactgactt tgtcagcaaa cgtgtcgtga ataacaacaa aagcgcgcgg
     6061 atataccgta ttgccatcag tgctattgat agcccgggca gcagtgaatt gcgcacccga
     6121 ccggtggatg gtgaactact tttcgccccc cgccagctgg cgttgcagac tggtgagagc
     6181 gagtatttta aattttacta tcatggtcca cgggataacc gcgagcgcta ctaccgggtc
     6241 tcatttcgcg aggtccccac tcgtaacctg acaaaacgca gcccaaccgg cggtgaggtc
     6301 agcacggagc cggtggtggt gatggatacc attctggtag tacgaccacg tcaggttcag
     6361 tttaaatggt ccttcgatca ggtgaccgga acggtaagta acaccggcaa cacgtggttt
     6421 aagctactga ttaaaccagg atgtgattcg accgaagagg aaggcgatgc ctggtatcta
     6481 cgtccgggag acgtggttca tcagcctgag ttacgtcagc cggggaatca ttatctggtc
     6541 tataacgaca aattcattaa gattagcgat tcctgtccgg ctaagccccc ttcggcggac
     6601 taagacttat ccatcggacg agggaagaat tccacctcag agactcaaaa atcgtttaaa
     6661 tgatgtggaa gcgatcgtca atgtcacctg taacgatgag ctttctcata tgcacaccac
     6721 agtgttcagg agacacggaa aggtaccagg cggcctgcta acgagtggtt tcttcactaa
     6781 ttaggacgta aaatcggtaa cggctggaaa tcattcaata ctcacactat cgaaagtaca
     6841 ccagccaacc gcgatacatc cctgcatacg acgtaccgca gttctcttta cgatttatag
     6901 ctgtactggt gaattatgag caatctgaat ccatgcatga cgtgtggtgc ctgttgtgca
     6961 tttttccgcg tctcttttta ctgggccgaa gctgacgatg ctggcggtac cattcccgcc
     7021 aggctcactg aacaaatatc cccttttcac cgatgcatga gcggtactaa tcagaaaaat
     7081 ccccgatgta ttgcccttgc aggaacaccg ggcaaaaatg cctgctgcac gatatatgaa
     7141 aatcgatcgt ccacatgcag agaattcgcc atgtctggtg agaacggaga agtcaatgag
     7201 gcttgcaatc gtgcaagagc taaatacggg ctaacaccgt tataaatata caataattaa
     7261 ttgcattgcc ccgccagctg taatagcggg gcttcgtttt tcagggacaa caaaacctga
     7321 aacttcttcc attttccagc aacaatgcct cttcttctgt agcgttctcc ggatctacat
     7381 catcctgaag tagcgcgata tgatcactga ctgtccgcat cagaatatga aaagcaacgt
     7441 ttttaaccta taaaaatggc gctatatttg cgccattttt atcattcaaa gcattatcta
     7501 tttgagccta aagggatctc aggatctggc tcatgggtaa ttctgtttcg taaatctctg
     7561 cgaataattt caatagacca gaaccagact aaatgtccaa aaatttcaga gacattctca
     7621 taccacggaa gatcaaagag aggtggcgta agtcccatga gagggaatga aatcatatga
     7681 acaaaaagtt gcgctaaagc acctgccagt aagccctgcc aaagtttaat tttcggaaat
     7741 acttcggcga ccacacagta accaacggca aaaactatcg aaaagattat gtgcgttaca
     7801 ccaacccagt taaagacatg cccggcgaag gtataaacag ccgaatttgg atctgtcagt
     7861 cccaaccaat cccgaagaaa aatgtacgga ggattgagaa agttacggga gcaatcaatt
     7921 tggcctgcag ccctgattaa tgattccgga ccacacgctg cattaaacat atcaaccgga
     7981 ctacgtggtg gcaatggaac ttctgccccc cacttaacaa atgcggaaac aacgccagca
     8041 atcagcccaa tgaatgcagc aagaccataa cgtctgcggt ttggtggagt ttgttcaaat
     8101 atattcatat ctaccctgct
//
LOCUS       DAZUCC010000209         8076 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZUCC010000209 DAZUCC010000000
VERSION     DAZUCC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.210"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..557
                     /locus_tag="ACHY09_004197"
     CDS             <1..557
                     /locus_tag="ACHY09_004197"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS3887087.1"
                     /translation="ILDKIQNGGVSWEMSPFDCFFDNIKQGKRLKKQDHIKGDIPFVM
                     AGITNNGVANFISNPHIIFPKNSITVDIFGNVFYRSYEFGAGDDTGVYWNDKERHSKE
                     NMLFLATSMACAIKDKYDYGRKLRSSQSKEIKMAVISKNGFPDDSAMKDLIYAVQKLV
                     IKDVVEYADKKLSAYRQVTSTKGV"
     gene            704..780
                     /locus_tag="ACHY09_004198"
     tRNA            704..780
                     /locus_tag="ACHY09_004198"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:738..740,aa:Arg,seq:tcg)
     gene            794..870
                     /locus_tag="ACHY09_004199"
     tRNA            794..870
                     /locus_tag="ACHY09_004199"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:828..830,aa:Arg,seq:tct)
     gene            1358..3313
                     /locus_tag="ACHY09_004200"
     CDS             1358..3313
                     /locus_tag="ACHY09_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFS3887088.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSGDGSTVISTTSEPEYYLVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADVAKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGASAFTTGADGTYSESAGASENSLRWGVGK
                     PLYQDLVSRTKAALAKNPKNRLLAVVWMQGEGDAAVGTHAQHSGLFTAMVNQFRTDLA
                     GQASQCTGGSASAVPWICGDTTYFWKQRHAEGYASVYGGYKGKESQNIFFVPLMTDEN
                     GANVQTNDPAEDPDLETIGYYGSKWRNDQKTWTSADRASHFSSWSRRGIISDRLATAI
                     LTHAGRVAVSTEKPASEPEAPPSPPSGAETTVTTTTGTTTLLSYSAGESEGKLSAQGW
                     RSGGGRAEIVDDAGGTGGKAMKITKETGKSSWYLENDAGTGAELLKKGGVLSCRFKVP
                     GALVANQYVMAFYWPVSSLPEGVSLGGDAGNNLLASFYIQTDATDLNVLYHNAKDSTQ
                     NQKLGTFGAFDNDWHTLAFRFAGNNSLQVTPVIDGKDGAAFTLTQSPVGAFPADKLRV
                     TDITKNATYPVLIDSVKVEVNNAEAGA"
     gene            3465..3659
                     /locus_tag="ACHY09_004201"
     CDS             3465..3659
                     /locus_tag="ACHY09_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFS3887089.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYSAVRYFRRRQADKSAAEKI
                     SASQSAGTRPEDPLIPEQPS"
     gene            3586..3957
                     /locus_tag="ACHY09_004202"
     CDS             3586..3957
                     /locus_tag="ACHY09_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFS3887090.1"
                     /translation="MRKSAPVSQPEPDLKTRSYRNNHLNNPFQRENPMSEIKTLVTAE
                     AVKDVIRSEEVRSVLKQKLRQNLEERLDAEVDAILDELLGAPAEPQPAPEGNTAENGD
                     IQPESPVVDVTEAQPEPGTML"
     gene            4035..4250
                     /locus_tag="ACHY09_004203"
     CDS             4035..4250
                     /locus_tag="ACHY09_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS3887091.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            complement(4488..5120)
                     /locus_tag="ACHY09_004204"
     CDS             complement(4488..5120)
                     /locus_tag="ACHY09_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887092.1"
                     /translation="MLIYPAADLRLQGRRAQPWDKTTTHKYRPGQYYDFRKHPELIET
                     HLEDFVEYSDRQAIQTFFSFVKWINSNSSAFESTDCMFSGTPKVDEYAPVFGCTHASS
                     GRFEFLFRDTKINQNERAVGWVLNKLSLYLQKERPDFCKGTFGIVPLMTEYTDSGGNE
                     FTGYRICVYFDAYGNGTEDTWTSLNIMFDGLMKATKRMSNETITGEMRPL"
     gene            5251..5784
                     /locus_tag="ACHY09_004205"
     CDS             5251..5784
                     /locus_tag="ACHY09_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS3887093.1"
                     /translation="MNTKIRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSEEKCAQVNAIERDKALAWVEKNIRVPLTDPQK
                     AGIASFCPYNIGPGKCFSSTFYRKLNTGDRKGACAEIRRWIYDGGQDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            6059..6631
                     /locus_tag="ACHY09_004206"
     CDS             6059..6631
                     /locus_tag="ACHY09_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFS3887094.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVEGMGMTWQSQHRKL
                     MERFKTCIIEMMIQLPGDTQRRLVICLALRKLAGWLQTINPNKVKPEIRDRVIQYQEE
                     CDDVLYEYWTKGVVVNPRRMSVMEELNQACADMQRDKNIVSVFATGLNEWKQVKAAHV
                     SKIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            6631..6780
                     /locus_tag="ACHY09_004207"
     CDS             6631..6780
                     /locus_tag="ACHY09_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887095.1"
                     /translation="MEWWLMISDKIITLTKTICVIVGVSFSLMMVALFLSIAWMALTS
                     AGLVG"
     gene            6788..7555
                     /locus_tag="ACHY09_004208"
     CDS             6788..7555
                     /locus_tag="ACHY09_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFS3887096.1"
                     /translation="MNNNSAKPFLPWPGGKRRLAKHILPLFPEHTMYVEPFCGGAALF
                     FMKKPSRAEIINDINSDIVNLYRVVKHHQEEFFRQFQWMPASREMWNELINQRTEYMT
                     DIQRAVRFFFIQKYGYAGRVDSRSFGVKTDAGRLIAESMARLLQEARDRLARATIEHL
                     DWEACIARYDRPTTLFYCDPPYYNLTGYGVDFPFGNYERMAELARTIKGKVIISLNDT
                     PEIRRLFEGMDIRQVEYCYSLDRCKGTPSSELIICNF"
     gene            7793..7978
                     /locus_tag="ACHY09_004209"
     CDS             7793..7978
                     /locus_tag="ACHY09_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFS3887097.1"
                     /translation="MRELKMKLCALMLPLVVSACGSTPPVQAPCVKPPPPPAWIMQPP
                     PDWQTLLNGIISPSETS"
BASE COUNT         2109 a         1825 c         2232 g         1910 t
ORIGIN      
        1 atattttaga taaaattcaa aatggtggcg tttcatggga aatgtcacca tttgattgtt
       61 ttttcgacaa cataaagcaa gggaagcggt tgaaaaagca agaccatatt aaaggtgata
      121 ttccttttgt aatggctggt ataacgaata atggcgttgc aaactttata tcaaatcctc
      181 atattatttt tcctaaaaat tcaattaccg ttgatatatt cgggaatgtc ttttatcgca
      241 gttatgaatt tggtgcaggg gatgatacag gtgtgtactg gaatgacaaa gagcggcatt
      301 caaaagaaaa tatgcttttc cttgcaacat caatggcatg cgccatcaag gataaatacg
      361 actacggcag aaagttaaga agctctcaaa gcaaagaaat aaaaatggca gtgatcagca
      421 aaaacggttt tccggacgat tccgcaatga aagatttgat ctatgcggtc cagaagcttg
      481 tcattaagga tgttgtcgaa tatgcagata aaaaactatc ggcatacagg caggttacat
      541 cgacgaaagg tgtttagttc aaatccccgc cttctctggc ggggttcggt cattgatgca
      601 tgaaaaaaac ggtttacaat cgtaaaaacc gccatatcat gataagagtg gttactttgc
      661 cacgcagctt aagcccgccg atgagcgggt tttttgtgac ataccgccat tagctcatca
      721 ggacagagcg ccagccttcg aagctggttg cgcggggggc gagtccccga tggcggtcca
      781 ttatctgcat catgcgttgt tagctcagca ggacagagca attgccttct aagcaatcgg
      841 tcactggttc gaatccagta caacgcgcca cacttatttt ttctggttca aatgttaaat
      901 ccttcacagt tcaggctgtt aatggttgtc tggctggtgg gggatttgat agaaaaactt
      961 cgcatggtga atccccctga gcggaggggc gtatcagcga caggtggttg gttatatccc
     1021 ttatcctgcg cgggttcagg tgctgatact gaactcaccg ggaggcaccc ggcaccatgc
     1081 gcatgatgat acagatacgc tactcatgcc cctctccgga ggggcttttt tatgggtaaa
     1141 aaaagcccgc gccgggaagc gcggcgcggg cggcaaggaa atacaataag tgagaattat
     1201 ttaccgggct cataataccc gattgttata aggctgcgca acggcgtggc ctttttcgta
     1261 ttgcgggctg tagtctccct tctgtcattg tcctgcaaca tctggacttc agcccgccct
     1321 tatctgattc agtacactat cccggccggg aggattcatg gcatttaaac attacgatgt
     1381 ggtcagggcg gcatcgccct ctgaccttgc ggaacgactg acacaaaaac tgaaggaggg
     1441 gtggcagcca tttggcagtc ctgtcgccat cacgccttat accctgatgc aggccattgc
     1501 ggcggaaggg gatgtcacca cgccggtggt ggtgaaaccg tcaggtgacg gaagcacagt
     1561 tatcagcacc accagcgaac cggagtatta ccttgttgtt gtcctggcgg ggcagtcaaa
     1621 cagcatggca tatggtgaag gtcttccgct gccggagaca tatgaccgtc cggacccgcg
     1681 tattaaacaa ctggcgcgtc gcagcacggt gacaccgggc ggtgccgcct gtaaatataa
     1741 cgacatcatt ccggcagacc attgtcttca tgatgtacag gacatgagcc gtcttaacca
     1801 tccgaaagca gatgttgcca aaggacaata cggcactgtt ggtcagggac tgcatatcgc
     1861 caaaaaactg ctgccgttta taccggcgaa tgcgggcatt ttgctggtac cgtgctgccg
     1921 tggtgcttcg gccttcacaa caggtgcaga cggcacatac agcgaatcag ccggtgcgtc
     1981 ggaaaattca ctgcgctggg gtgtgggtaa gccgctgtat caggatttgg tgagccggac
     2041 aaaagccgca ctggcgaaga acccgaaaaa ccgtctgctt gcggtggtgt ggatgcaggg
     2101 agaaggtgat gcggcagtgg ggacgcatgc gcagcattcg ggactgttca ctgcgatggt
     2161 gaatcagttc agaacggacc ttgccggtca ggcatcacag tgcacgggag gcagcgcatc
     2221 agcggtaccg tggatttgcg gggacacaac gtatttctgg aagcagcgtc atgcggaagg
     2281 gtatgcatcc gtatacggag ggtataaggg taaggagtca cagaacattt tctttgtacc
     2341 gttaatgacg gatgagaatg gtgcgaatgt gcagaccaat gacccggcag aagacccgga
     2401 cctggaaacc atcggttatt acggttcaaa atggcgtaat gaccagaaga cctggacatc
     2461 tgcggacagg gccagtcatt tcagttcatg gtcacgccgc gggattattt ccgaccgtct
     2521 ggcaacggcg attctgacgc atgccggacg tgttgccgtc agcacggaga agcctgcttc
     2581 tgagccggaa gcgccgccgt ctccgccgtc aggggcagaa acgacagtca ccacgacaac
     2641 gggcaccaca acgctgctgt cttacagtgc cggtgagtca gaagggaagc tgtcagcgca
     2701 gggctggcgc tctggtggcg gcagggctga aatcgtggat gacgccggag gtacaggcgg
     2761 taaggcgatg aagataacca aggaaaccgg taagtcctcc tggtatctgg agaatgatgc
     2821 gggtacgggg gcggaactgc tgaaaaaagg tggtgtgctc agttgtcgct ttaaggttcc
     2881 gggcgcactg gtggcgaatc agtatgtcat ggcgttttac tggccggtgt cctccctgcc
     2941 ggagggagtc agcctgggtg gtgatgcggg gaataacctg ctggcatcat tctatatcca
     3001 gacagatgca acagacctga atgtgctgta tcacaatgcg aaggactcga cacagaatca
     3061 gaagctgggg acgtttggcg cattcgataa cgactggcac acgctggcat tccgctttgc
     3121 aggaaataac agtcttcagg tgactccggt tattgatggt aaggatggtg cggcgttcac
     3181 cctgacgcag tcaccggtag gcgctttccc ggcagacaag ctgcgtgtaa cggatattac
     3241 caaaaatgcg acatatccgg tgctgattga cagtgtgaag gtggaagtga acaacgcgga
     3301 agccggagcg tgataaaaaa agccgccagt aaagaaggag aagggatgaa ctgtgtccac
     3361 tgcataaata ctggcggatg gtaaaacgac catgatgaca ccgaagttag cggtgttttt
     3421 ctacaaaaat aagtaaatca tgtcaactat cacggtgaga aaccatgaca tttctgaacc
     3481 agttaatgct gtacttctgt acggtggtct gtgtgctgta tctcctttcg ggtgggtaca
     3541 gcgccgtgcg ttatttccgg cgcagacagg ccgacaaaag cgccgctgag aaaatcagcg
     3601 ccagtcagtc agccggaacc agacctgaag acccgctcat accggaacaa ccatcttaac
     3661 aacccctttc agcgagaaaa tcccatgtca gaaattaaaa ccctggtcac tgcagaggca
     3721 gtgaaggacg tcattcgctc tgaagaagtc agaagcgtac tgaaacagaa acttcgccag
     3781 aatctggagg agcgtctcga tgcggaagtg gatgccattc tggatgaact gctcggtgca
     3841 ccggcagagc cacagccagc cccggaagga aacacagcag agaacggtga tattcagccg
     3901 gagtcaccgg tggttgatgt gactgaagca caacctgaac cgggcacaat gctgtaacgg
     3961 tgagtcaggg ccatcagtaa agcgctgctg gcctttttca tggttgtgag cttccgtatt
     4021 gcgggagacg gggtatgtac cagatggaaa aaatcacaac aggtgtgtcc tacaccacgt
     4081 cagcggtggg aacggggtac tggttcctgc agttgctgga cagggtatcc ccgtctcagt
     4141 gggcggcaat aggcgtactg gggagtctgc tgtttggtct gctgacgtac ctgacgaacc
     4201 tgtatttcaa aattaaagaa gaccggcgta aggcggcccg gggagagtag acgatgaact
     4261 ctgaagaaat gaatcagcgt ataagtcggc tggaaaacga aattgctgaa ctgaataaaa
     4321 aactgtcggt actggtgcct tctgacgatg aaaaaaaacg ccgcgatgaa cagtatgcgg
     4381 cattttacga ttattgccgg aaagtgatga acagaaatct ggcagagtgt ttccgtattc
     4441 aaaaaaacag agagcgctct gcaaatagtg agattctgga taatttttta aaggggacgc
     4501 atctcgccag tgattgtttc attgctcatt cttttggtgg ctttcatgag gccatcaaac
     4561 atgatgttga gagaagtcca ggtatcctct gttccattcc cgtaagcatc gaaatagaca
     4621 caaatacgat aaccggtgaa ctcgttaccg ccggagtcgg tgtattccgt catgagggga
     4681 acgatgccaa aggttccttt acaaaaatcg ggccgttcct tctgaaggta gagcgatagt
     4741 ttgttaagta cccatccgac agctcgctca ttttggttta ttttcgtatc gcgaaacaaa
     4801 aattcgaagc ggccagatga cgcatgggtg caaccaaaca ccggggcgta ttcatcaacc
     4861 tttggtgttc ctgaaaacat gcagtccgtg ctctcgaatg cagatgaatt gctgttgatc
     4921 cacttaacaa aagaaaaaaa ggtctgaatc gcttgtctgt ctgaatattc aacaaaatcc
     4981 tcaaggtgtg tctcgatcag ttccgggtgt ttacgaaagt cataatattg accgggacga
     5041 tatttatgag tggttgtttt atcccatggt tgtgctctgc gtccctgtaa acggaggtct
     5101 gccgctggat agataagcat aagtaatctc ctggtggaat gtaatccacg atcatccggg
     5161 gaaattaaga acccgccagt gcccaccact ggtggggtga aggcttaaca tatccgggta
     5221 ttcggaaccg ataaatcctg ataaatatcc atgaacacaa aaatcagata cggcctgtcg
     5281 gctgctgttc tggcgctgat tgccgcgggg gcttctgcgc cagaaatcct cgaccagttt
     5341 cttgacgaaa aggaaggcaa ccacaccacg gcataccgtg atggtgcggg tatctggacc
     5401 atctgtcgtg gtgccacccg ggtggatggt aaacctgttg ttcctggcat gaagttgtcg
     5461 gaggaaaaat gcgctcaggt taatgccatt gagcgtgata aggcgctggc atgggtggag
     5521 aaaaacatca gagtgccact gaccgaccca cagaaagcgg gtatcgcgtc attctgtcct
     5581 tacaacattg gccccggtaa gtgtttcagc tcaacctttt accggaaact gaacaccgga
     5641 gaccgtaaag gagcatgtgc tgaaattcgt cgctggatat atgacggtgg tcaggactgc
     5701 cgtatccgct caaacaactg ttacggtcag gtatcccggc gtgatcagga gagtgcgctg
     5761 gcctgctggg gaattgacag gtaagcagaa tattttgctg aaaaatgaca tttgctcaca
     5821 cggacggata acacgaaatc ctgtgaactg gcaaaatgta agtgaataaa gtcaggatca
     5881 ttgtttcacg cagaggcacc gtaatggtgt ctttgtcatt tctgcgcttc gcacaagcgt
     5941 aaataaacca aagaaccttt caggatgagc cctggtggat aaccgacagt ggtctggtta
     6001 accctctttg ggctggttat tcctgtgcgc agggttcatc actaaaagga aataaccgat
     6061 gaatatgatg accgtgccgt ttcacggcga ttctctttat gtggttaacc ataacggtga
     6121 gccatacgtt cccatgaaac ctgtcgttga gggaatgggt atgacctggc agtctcaaca
     6181 ccgcaaattg atggagagat ttaagacctg catcatcgaa atgatgattc agctccccgg
     6241 tgatactcag cgtcgtctgg ttatctgtct tgctttacgt aaacttgcag gttggctgca
     6301 aaccatcaac ccaaataaag ttaaacctga aatccgcgac agagtcattc agtatcagga
     6361 agagtgcgac gatgttctct atgaatactg gacgaagggg gttgtcgtta atccccgtcg
     6421 aatgagtgtg atggaagaac tcaatcaggc ttgcgctgac atgcaacggg ataaaaacat
     6481 tgtcagtgtg tttgctaccg ggctgaatga gtggaaacag gttaaagctg cgcatgtatc
     6541 aaaaatccgc acattgataa acgaagcgaa tctgctgatt gattttgtcc tggctgatac
     6601 aggcaaaggg aaaataacaa aggcggattg atggagtggt ggctaatgat atcggataaa
     6661 atcataacgc tgacaaagac catttgtgtg attgtcggcg tttcattttc gctaatgatg
     6721 gttgctcttt ttctttccat cgcctggatg gcgttgactt cggccggtct ggtggggtga
     6781 gggggatatg aacaataact ctgcaaaacc gtttctcccg tggcccggag ggaagcgtcg
     6841 tctggcaaaa cacattctgc cgttatttcc tgagcacacg atgtatgtgg aaccgttctg
     6901 tggtggtgct gcgctgttct ttatgaaaaa accgagtcgc gctgaaatca tcaacgatat
     6961 aaacagtgat atcgttaatc tctaccgtgt ggtgaagcac catcaggagg agtttttccg
     7021 ccagtttcag tggatgcccg ccagtcgtga gatgtggaat gaactgataa accagcgcac
     7081 ggagtacatg acggatatac agcgagctgt tcgtttcttt tttatccaga agtatggata
     7141 tgcagggaga gtggatagcc ggtcatttgg tgttaaaacg gatgcagggc ggttgattgc
     7201 tgagagcatg gcgcggttat tacaggaggc tcgtgaccgg ctggcgcggg caaccattga
     7261 acaccttgac tgggaggctt gtatcgcacg gtatgaccgc ccgacaacgc tgttttactg
     7321 tgacccgccg tattacaacc ttacgggtta tggtgttgat tttccattcg gcaattatga
     7381 gcgcatggca gaacttgccc gcacgataaa aggcaaagtg attatatccc tgaacgatac
     7441 gccggaaatc aggcgattgt ttgaggggat ggacattcgt caggttgagt attgttactc
     7501 gctggaccgt tgtaaaggca ccccatccag tgagctgata atctgcaatt tctgacaggg
     7561 ggtatggtga gcataaaccg aatgcttttc gcgtttaccg ttattctgct ggtggtctgt
     7621 ggtgcgctgt ggctggcagc aaaccattac cgcgataatg cgatcatcta caaagcgcag
     7681 cgcgataaaa aagccagcga gctggagctg gcaaacgcaa ccattactga catgcagcag
     7741 cgccagcgtg atgttgctgc gctcgatgcc agatactcga gggaattagc tgatgcgaga
     7801 gctgaaaatg aaactctgcg cgctgatgtt gccgctggtc gtaagcgcct gcgggtcaac
     7861 gccacctgtc caggctccgt gcgtgaagcc cccaccacct ccggcgtgga taatgcaacc
     7921 gccccccgac tggcagacat tgctgaacgg gattatttca ccctcagaga ccagctgatg
     7981 acgatgcaga agcaactgga aggggcgcag gaatatatac gcactcagtg cactaagctg
     8041 gctttttatt atccggagga tacatgaata aattac
//
LOCUS       DAZUCC010000210         7968 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZUCC010000210 DAZUCC010000000
VERSION     DAZUCC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.211"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..1068
                     /gene="cysB"
                     /locus_tag="ACHY09_004210"
     CDS             94..1068
                     /gene="cysB"
                     /locus_tag="ACHY09_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFS3887098.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEIMFKDIKLPEK"
     gene            1387..1527
                     /locus_tag="ACHY09_004211"
     CDS             1387..1527
                     /locus_tag="ACHY09_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFS3887099.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            1530..1697
                     /gene="yciX"
                     /locus_tag="ACHY09_004212"
     CDS             1530..1697
                     /gene="yciX"
                     /locus_tag="ACHY09_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFS3887100.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQQKQ"
     gene            1811..1906
                     /gene="ymiC"
                     /locus_tag="ACHY09_004213"
     CDS             1811..1906
                     /gene="ymiC"
                     /locus_tag="ACHY09_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFS3887101.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2056..4731
                     /gene="acnA"
                     /locus_tag="ACHY09_004214"
     CDS             2056..4731
                     /gene="acnA"
                     /locus_tag="ACHY09_004214"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFS3887102.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGNITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDDVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4795..5385)
                     /gene="ribA"
                     /locus_tag="ACHY09_004215"
     CDS             complement(4795..5385)
                     /gene="ribA"
                     /locus_tag="ACHY09_004215"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFS3887103.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5555..6319
                     /gene="pgpB"
                     /locus_tag="ACHY09_004216"
     CDS             5555..6319
                     /gene="pgpB"
                     /locus_tag="ACHY09_004216"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFS3887104.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6468..6776
                     /gene="lapA"
                     /locus_tag="ACHY09_004217"
     CDS             6468..6776
                     /gene="lapA"
                     /locus_tag="ACHY09_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFS3887105.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            6783..7952
                     /gene="lapB"
                     /locus_tag="ACHY09_004218"
     CDS             6783..7952
                     /gene="lapB"
                     /locus_tag="ACHY09_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFS3887106.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
BASE COUNT         1974 a         1870 c         2086 g         2038 t
ORIGIN      
        1 gttagcactt tttttattat taaatcgtat tagtcacccg ccaggtgtga cgaaaaaacg
       61 atgttctgat ggcgtctaag tggatggttt aacatgaaat tacaacaact tcgctatatt
      121 gttgaggtgg tcaatcataa cctgaatgtc tcatcaacag cggaaggact ttacacatca
      181 caacccggga tcagtaaaca agtcagaatg ctggaagacg agctaggcat tcaaattttt
      241 tcccgaagcg gcaagcacct gacgcaggta acgccagcag ggcaagaaat aattcgtatc
      301 gctcgcgaag tcctgtcgaa agttgatgcc ataaaatcgg tcgccggaga gcacacctgg
      361 ccggataaag gctcgctgta tatcgccacc acgcataccc aggcacgcta cgcgttacca
      421 aacgtcatca aaggctttat tgagcgttat ccccgcgttt ctttgcatat gcaccagggc
      481 tcgccgacac aaattgctga tgccgtctct aaaggcaatg ctgatttcgc tatcgccaca
      541 gaagcgttgc atctgtatga agatctagtg atgttaccgt gctaccactg gaatcgggct
      601 attgtagtca ctccggatca cccgctggca ggcaaaaaag ccattaccat tgaagaactg
      661 gcgcaatatc cgttggtgac atataccttc ggctttaccg gacgttcaga gctggatact
      721 gcctttaacc gcgcagggtt aacgccgcgt atcgtcttca cggcaacgga tgctgacgtc
      781 attaaaactt acgtccggtt agggctgggg gtaggggtca ttgccagcat ggcggtggat
      841 ccggtcgccg atcccgacct ggtgcgcgtt gatgctcacg atatcttcag ccacagtaca
      901 accaaaattg gttttcgccg tagtactttt ttgcgcagtt atatgtatga tttcattcag
      961 cgttttgcac cgcatttaac gcgtgatgtc gttgatgcgg ctgtcgcatt acgctctaat
     1021 gaagaaattg agatcatgtt taaagatata aaactgccgg aaaaataatt tcttgcgtta
     1081 ttttcggcac cttttatgta gcgaaggtgc cggaatatat tctcttttgt tgcttatttg
     1141 tataccgtgc acaatatttt tatccctcat atttataggg taaattacct gtaaagtagt
     1201 gtctaattac ccgcctcgca aactcgagtt ttcaaatatc tatttcaggt caaaagattg
     1261 aattattcac tggagacgat tcgtaaattc gctggatttt tgactaaagt ttctttagga
     1321 tttgtctcac cgtatgatta attacaccaa tcgttcaacg tcttgctgat aagtgatggc
     1381 gattatatga ggttagcaat gccttctgga aatcaggaac cccgccggga ccctgaatta
     1441 aaacgcaaag cctggctggc ggtatttctt ggttctgctc ttttctgggt ggttgtcgca
     1501 ctgctgattt ggaaagtgtg gggataacta tggtcggtca ggagcaactg gagtcgtcac
     1561 cattatgtca gcatagtgac aatgaaacag aaacgaaacg ggaatgttcc gtcgttattc
     1621 cagacgactg gcaactaaca tcgcagcagc aagcctttat agaactgttt gctgaagatg
     1681 atcagcagaa acaataatta tcatcattct tattacccat ttttaatgaa ttaaagggct
     1741 tttaatacac cgcagcaata acaggttgag ttatctcaac acaaagtaat aaccgttaaa
     1801 ggtgtagcct atgatcaaca caaatatgaa atattggtcc tggatgggcg cattttctct
     1861 gtcgatgctc ttctgggccg aactcctctg gatcattact cactgatccc tgaccccgct
     1921 gtttgctttg tctcaaggtt tctcctcttt tatcaatttg ggttgttatc aaatcgttac
     1981 gcgatgtttg tgttatcttt aatattcacc ctgaagagaa tcagggcttc gcaaccctgt
     2041 cattaaggag gagctatgtc gtcaacccta cgagaagcca gtaaggacac gttgcaggcc
     2101 aaagataaaa cttaccacta ctacagcctg ccgcttgctg ccaaatcact gggcaatatc
     2161 acccgtctgc ctaagtcact caaagttttg ctcgaaaacc tgctgcgctg gcaggatggt
     2221 aactcggtta ccgaagagga tatccacgcg ctggcgggat ggctgaaaaa tgcccatgct
     2281 gaccgtgaaa ttgcctaccg cccggcaagg gtgctgatgc aggactttac cggcgtacct
     2341 gccgttgttg atctggcggc aatgcgcgaa gcggttaaac gcctcggtgg cgatactgca
     2401 aaggttaacc cgctctcacc ggtcgacctg gtcattgacc actcggtgac cgtcgatcgt
     2461 tttggtgatg atgaggcatt tgaagaaaac gtacgcctgg aaatggagcg caaccacgaa
     2521 cgttatgtgt tcctgaaatg gggaaagcag gcgttcagtc gttttagcgt cgtgccgcca
     2581 ggcacaggca tttgccatca ggttaacctc gaatatctcg gcaaagcagt gtggagcgaa
     2641 ttgcaggacg gtgaatggat tgcttatccg gatacactcg ttggtactga ctcgcacacc
     2701 accatgatca acggccttgg cgtgctgggg tggggcgttg gtggcatcga agccgaagcc
     2761 gcaatgttag gccagccggt ttccatgctt atcccggacg tagtgggctt taaactcacc
     2821 ggaaaattac gtgaaggtat taccgccaca gacctggttc tcaccgttac ccaaatgctg
     2881 cgcaaacatg gcgtggtggg gaaattcgtc gaattttatg gtgatgggct ggattcacta
     2941 ccgctggcgg atcgcgccac cattgccaat atgtcgccag aatatggtgc cacctgtggc
     3001 ttcttcccaa tcgatgctgt caccctcgat tacatgcgtt taagcgggcg cagcgaagat
     3061 caggtcgagt tggtcgaaaa atatgccaaa gcgcagggca tgtggcgtaa cccgggcgat
     3121 gaaccaattt ttaccagtac gttagaactg gatatgaatg acgttgaagc gagcctggca
     3181 gggcctaaac gcccacagga tcgcgttgca ctgcccgatg taccaaaagc atttgccgcc
     3241 agtaacgaac tggaagtgaa tgccacgcat aaagatcgcc agccggtcga ttatgttatg
     3301 aacggacatc agtatcagtt acctgatggc gcggtggtca ttgctgcgat aacctcgtgc
     3361 accaacacct ctaacccaag tgtgctgatg gccgcaggct tgctggcgaa aaaagctgta
     3421 actctgggcc tcaagcggca accgtgggtc aaagcgtcgc tggcaccagg ctcgaaagtt
     3481 gtttctgact atctggcaaa agcgaaattg acaccgtatc tcgacgaact ggggtttaac
     3541 cttgtggggt acggttgtac cacctgtatt ggtaactctg ggccgctgcc cgatcctatc
     3601 gaaacggcaa tcaaaaaagg cgatttaacc gtcggtgcgg tgctgtccgg caaccgtaac
     3661 tttgaaggcc gtatccatcc gctggttaaa actaactggc tggcctcgcc gccgctggtg
     3721 gttgcctatg cgctggcggg aaatatgaat attaacctcg cttctgagcc tattggtcac
     3781 gatcgtaaag gcgacccggt atatctgaaa gatatctggc cctcggcaca agaaattgcc
     3841 cgtgcggtag aacaagtctc cacagaaatg ttccgcaaag agtacgcaga agtttttgaa
     3901 ggcacagcag agtggaagga aattaacgtc acacgatccg atacctacgg ttggcaggaa
     3961 gattcaacct atattcgctt atcgccattc tttgatgaaa tgcaggcaac accagcacct
     4021 gtggaagata ttcacggtgc gcggatcctc gcaatgctgg gggattcagt caccactgac
     4081 catatctctc cggcgggcag tattaagccc gacagcccgg cgggtcgata tctacaaggt
     4141 cggggtgttg agcgaaaaga ctttaactcc tacggttcgc ggcgtggtaa ccatgaagtg
     4201 atgatgcgcg gcaccttcgc caatattcgc atccgtaatg aaatggtgcc tggcgttgaa
     4261 ggggggatga cgcggcattt acctgacagc gatgatgtct ctatctatga tgccgcgatg
     4321 cgctataagc aggagcaaac gccgctggcg gtgattgccg ggaaagagta tggatcaggc
     4381 tccagccgtg actgggcggc aaaaggtccg cgtctgcttg gtattcgtgt ggtgattgcc
     4441 gaatcgtttg aacgaattca ccgttcgaat ttaattggca tgggcatcct gccgctggaa
     4501 tttccgcaag gcgtaacgcg taaaacgtta gggctaaccg gggaagagaa gattgatatt
     4561 ggcgatctgc aaaacctaca acccggcgcg acggttccgg tgacgcttac gcgcgcggat
     4621 ggtagccagg aagtcgtacc ctgccgttgt cgtatcgaca ccgcgacgga gttgacctac
     4681 taccagaacg acggcatttt gcattatgtc attcgtaata tgttgaagta acaagtattt
     4741 gcttgccggt tattttgctt ccggcaagca aatgaattac acaatgcaag agggttattt
     4801 gttcagcaaa tggcccattt tctcggcttt ggtatcgaga taatgttcgt tattggggtt
     4861 acgacctaca atcaatggta cgcgttcaac aatattaatc cctgcttcgg tcagaatttc
     4921 gacttttttc gggttattgg ttaacaagcg gacttcattg acgccaagga gtttgaacat
     4981 atcagcgcaa agagtgaagt cgcgctcatc agcggcgaag cctaactggt ggttagcctc
     5041 tacagtatcg taaccttgat cctgcagtgc ataagcgcgg attttattca gcagaccaat
     5101 gttacgacct tcctgacggt gatacagcaa aataccacgg ccttcttcgg caatttgcgt
     5161 cagtgccgct tcgagctgga agccacaatc gcagcgcaag ctgaacaggg cgtcaccggt
     5221 cagacattcg gaatggacgc gcgcaagtac cggggtatgc ccggagatat cgccatagac
     5281 tagcgcgaca tgatcgtgtc cggttgccag ttcttcaaat cccaccatca ggaaatcgcc
     5341 ccatggggtt ggcagtttgg cttctgccac acgtttaagc tgcatgaaat tctccagata
     5401 atgctggttc tgtattggct tattttgcca taacgagaag gggttcacct aatcacgacg
     5461 cgtcgatcgt tcacggaatg gcacaaatct gtcaattttt cctggaactg gcgttttcag
     5521 ttatgattgt gggacttatc aaaaaggaga ggccatgcgt tcgattgcca gacgtaccgc
     5581 agtgggagct gcactattgc ttgtcatgcc agtagccgta tggatttctg gttggcgttg
     5641 gcaacctgga gaacaaagtt ggctactaaa agcggctttt tgggttactg aaactgtcac
     5701 ccagccctgg ggcgtcatta cacatttgat tttattcggc tggtttctct ggtgtctgcg
     5761 ttttcgcatt aaggctgcct ttgtattatt tgccattctg gcggcggcaa ttcttgtggg
     5821 acaaggcgtt aaatcctgga tcaaagacaa agtccaggaa ccacgacctt ttgttatctg
     5881 gctggaaaaa acacatcata ttccggttga tgagttctac actttaaagc gagcagaacg
     5941 cggaaatctg gtgaaagaac agttggctga agagaaaaat atcccacaat atttgcgttc
     6001 acactggcag aaagagacgg ggtttgcctt tccttccggt cacacgatgt ttgctgccag
     6061 ttgggcgctg ctggccgttg gtttgctgtg gccgcgtcgg cgaacgttaa ccattgctat
     6121 cttgttggtc tgggcaacgg gagtcatggg aagccgcctg ctgctcggga tgcattggcc
     6181 acgcgatctg gtggtagcta cgttgatttc gtgggcgctg gtggcggtgg caacgtggct
     6241 tgcgcaacga atttgtgggc cattaacacc acctgcggaa gaaaatcgcg aaatagcgca
     6301 acgagaacaa gaaagttaac gctggttgat tttccgaatt tagcccttaa atcatcatca
     6361 atgcgcgtgg atgccatttc gcagacggcg cgaaaatggt actttaaagg gctattgcgg
     6421 taagttgacc ataatttatt cgctctaacc acataacggg aagtaatgtg aaatatttac
     6481 tcattttctt actggtgtta gcgatcttcg tgatttcggt cacgttaggt gcgcagaacg
     6541 atcaacaggt gacgtttaat tatctgttag cgcaagggga gtaccgtatt tccacattgc
     6601 tggcggtatt gtttgctgcg gggtttgcta tcggttggct gatttgcggt ctgttctggc
     6661 tgcgagttcg tgtttccctg gcgcgcgctg aacgtaaaat aaagcgactg gaaaaccagc
     6721 tttcacccgc gactgacgtg gctgtagcgc cgcattcgtc agcggcgaag gaataacttt
     6781 ctatgctgga gttgttgttt ctgcttttgc ctgtagccgc tgcctatggc tggtatatgg
     6841 gccgcagaag tgcgcaacaa aacaagcaag atgaagccaa ccgcttgtcg cgtgattacg
     6901 tagcgggggt taacttcctg cttagtaatc aacaggataa agcggtagac ctgtttctcg
     6961 atatgcttaa agaggatacg ggtaccgttg aagcccacct tacgctagga aacctgttcc
     7021 gttcgcgtgg cgaagttgat cgcgctattc gcatccatca gaccctaatg gaaagcgcct
     7081 cgctgaccta tgaacagcgt ctgttggcga ttcaacaact ggggcgtgat tacatggccg
     7141 ccgggttata cgaccgcgcg gaagacatgt tcaatcagct gaccgatgaa actgacttcc
     7201 gcattggcgc gctgcaacag ttgctacaaa tctaccaggc taccagcgag tggcagaaag
     7261 caattgatgt tgccgaacgt ctggtgaagt tgggtaaaga taagcagcgc gtcgaaattg
     7321 cccatttcta ctgtgagtta gccctgcagc atatggccag cgacgatctc gatcgcgcca
     7381 tggccttgtt aaaaaaaggt gcggcggcag ataaaaacag cgcccgcgta tccatcatga
     7441 tgggacgcgt atttatggcg aaaggagaat acgccaaagc cgtcgaaagt ctgcaacgcg
     7501 tgatatctca ggacagagaa ctggtcagcg aaacgctgga aatgttgcaa acctgctatc
     7561 agcagttggg taaaactgcc gaatgggcag aattcctgca gcgcgcggtg gaagagaaca
     7621 ccggtgccga tgctgaattg atgcttgcgg atattatcga agcgcgcgac ggtagtgagg
     7681 ccgcacaggt ctatattacg cgtcagcttc agcgtcatcc gaccatgcgt gtgttccata
     7741 agctaatgga ttaccactta aatgaagcgg aagaagggcg tgccaaagag agtctgatgg
     7801 tgctgcgtga catggttggc gagaaggtgc gtagtaagcc tcgttatcgc tgccagaaat
     7861 gtggttttac cgcatacacc ctctactggc attgtccgtc ttgtcgggcc tggtcaacca
     7921 ttaaaccgat tcgcggtctt gatggcctgt aatttttaaa aaaatccg
//
LOCUS       DAZUCC010000211         7853 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZUCC010000211 DAZUCC010000000
VERSION     DAZUCC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.212"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..191
                     /gene="kdpF"
                     /locus_tag="ACHY09_004219"
     CDS             102..191
                     /gene="kdpF"
                     /locus_tag="ACHY09_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFS3887107.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            191..1864
                     /gene="kdpA"
                     /locus_tag="ACHY09_004220"
     CDS             191..1864
                     /gene="kdpA"
                     /locus_tag="ACHY09_004220"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFS3887108.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFIICTGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLALMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1887..3935
                     /gene="kdpB"
                     /locus_tag="ACHY09_004221"
     CDS             1887..3935
                     /gene="kdpB"
                     /locus_tag="ACHY09_004221"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFS3887109.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTFISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPVDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3944..4516
                     /gene="kdpC"
                     /locus_tag="ACHY09_004222"
     CDS             3944..4516
                     /gene="kdpC"
                     /locus_tag="ACHY09_004222"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFS3887110.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4509..7193
                     /gene="kdpD"
                     /locus_tag="ACHY09_004223"
     CDS             4509..7193
                     /gene="kdpD"
                     /locus_tag="ACHY09_004223"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFS3887111.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGEKLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7190..>7853
                     /gene="kdpE"
                     /locus_tag="ACHY09_004224"
     CDS             7190..>7853
                     /gene="kdpE"
                     /locus_tag="ACHY09_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFS3887112.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGY"
BASE COUNT         1583 a         2065 c         2360 g         1845 t
ORIGIN      
        1 tttttatact ttttttacac cccgcccgca gatttttgcg aaatctttgc agccagaatt
       61 ctactcttcc ggtatcactt ttaggccact ggaggtgcac tgtgagtgca ggcgtgatag
      121 ccggcgtgtt gctggtgttt ttattactgg gttatctggt ttatgccctg atcaatgcgg
      181 aggcgttctg atggctgcgc aagggttctt actgattgcc acgtttttac tggtgttaat
      241 ggtgctggcg cgtcctttag gcagcgggct ggcgcggctg attaatgaca ttcctcttcc
      301 cggtacgacg ggcgttgagc gcgtactttt tcgcgcgctt ggcgtctctg accgcgagat
      361 gaactggaag cagtatctct cagccattct tggcctgaat atactggggc tggcggtgct
      421 gttttttatg ttgctcggtc agcactatct gccgcttaat ccacagcagt tgccaggact
      481 gtcgtgggat ctggcgctga ataccgccgt cagctttgtc acaaatacca actggcaatc
      541 ttacagcggt gaaaccacgt tgagctattt cagccagatg gcgggcttaa cggtgcaaaa
      601 ctttctttct gccgccagcg ggattgcggt gatttttgcc ctcatacgtg catttacccg
      661 ccagagcatg agcacactcg ggaatgcctg ggtcgatctg ctacgcatca cgttatgggt
      721 gctagtccct gtggcgttgt tgatcgcatt gttttttatt caacaaggtg cgctgcaaaa
      781 ctttctgccc tatcaggcgg tgaataccgt tgaaggagcg caacagctgt tacccatggg
      841 gcctgtagct tctcaggaag cgatcaagat gctcggcact aacggcggtg gcttctttaa
      901 tgccaactcg tcgcatccgt ttgaaaaccc aaccgcactg accaacttcg tgcagatgct
      961 ggcgatcttc ttgatcccaa cagcgctgtg ctttgccttt ggcgaagtgg cgggcgatcg
     1021 ccgtcagggg cgcatgttgc tgtgggcgat gtcagtgatt tttatcatct gcacaggcgt
     1081 ggtgatgtgg gcagaagttc agggtaatcc gcatctgctg gcactgggcg cggacagcag
     1141 catcaatatg gaaggtaaag agagtcgttt cggcgtgctg gtcagtagcc tgtttgcggt
     1201 cgtgactacg gcggcttcct gtggcgcggt gattgcgatg catgattcgt ttaccgctct
     1261 tggtggcatg gtgccgatgt ggctgatgca aattggtgaa gtggtgttcg gcggtgtcgg
     1321 ttctggtctt tacggcatga tgctgtttgt cctgctggcg gtgtttattg ctgggctgat
     1381 gattggtcgt acaccggaat atctgggtaa aaaaatcgac gtacgcgaga tgaaactgac
     1441 cgcgctggcg attctggtca ccccaacgct ggcgctgatg ggcgcggcgc tggcaatgat
     1501 gaccgacgcc ggacgtagcg ccatgctcaa ccctggcccg catggtttta gcgaagtgct
     1561 gtacgccgtg tcatccgccg ctaacaacaa cggcagcgcc tttgccggat taagcgccaa
     1621 ctctccattc tggaactgtt tgctggcgtt ctgcatgttt gtcggtcgct tcggggtaat
     1681 tatcccggtg atggcaattg ccggttcgct ggtgagtaaa aagagccagc ccgccagctc
     1741 cggcacgctg ccaacgcacg gcccgctgtt tgttggcctg ttaatcggca ccgtgttgct
     1801 ggttggcgca ctgaccttta tccctgccct ggcgcttggt ccggtggcgg aatatctctc
     1861 ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca actggcgcta ttcgaaccaa
     1921 cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt aaacccgcag gcgcaatggc
     1981 gtaatccagt gatgtttatc gtctggatcg gcagtctgtt aaccaccttt attagcattg
     2041 caatggcaag cggcgcgatg cctggcaatg cgctgtttag cgcggcaatt agcggctggc
     2101 tgtgggtcac cgtactgttc gctaatttcg ccgaggcgct ggcagaaggc cgcagtaaag
     2161 cgcaagccaa tagtctgaaa ggggtgaaaa aaactgcctt tgcccgcaag ctgcgtgagc
     2221 cgaaatatgg cgctgcggcg gacaaagttc ccgcagatca actgcgtaaa ggcgatatcg
     2281 tactggtaga agctggcgat attatcccct gcgatggtga agtcattgaa ggcggcgcat
     2341 cggtcgatga aagcgccatc accggggaat cggctccggt gatccgtgaa tccggcggcg
     2401 attttgcctc cgtcactggc ggcacgcgta ttctttctga ctggctggtg attgagtgca
     2461 gcgttaaccc cggcgagaca tttctggatc ggatgatcgc gatggtggaa ggcgcgcagc
     2521 gacgcaaaac gccgaacgag attgccctga ccatcctgct gattgccctg actatcgtct
     2581 ttttactggc aaccgccacg ctgtggccgt tttccgcatg gggtggtaat gcagtcagcg
     2641 taacggtact ggtggcgctg ttggtctgtc tgatcccaac cactattggc ggcctgttgt
     2701 cagcgatcgg cgtcgccggg atgagccgga tgttgggcgc gaatgtgatt gccaccagcg
     2761 ggcgtgcagt tgaagcggcg ggcgacgtgg acgtcctgct gctggataaa actggcacca
     2821 tcaccctcgg taaccgtcag gcatcagagt ttatccctgc gcagggcgta gaggaaaaaa
     2881 cgctggccga cgcagcacaa ctggcttcgc tggccgacga aacgccggaa ggccgcagta
     2941 ttgtgatcct cgccaagcag cgctttaacc tgcgcgagcg cgatgtgcag tcgcttcacg
     3001 ccacttttgt accgtttact gcgcaaagtc gaatgagcgg gatcaatatc gacaaccgca
     3061 tgatccgtaa aggttctgtc gatgccattc gccgccatgt tgaggctaac ggtggtcact
     3121 tccctgtcga tgttgatcaa aaagtcgatc aggttgcgcg tcagggggcc acgccgctgg
     3181 tggtggtgga aggttctcgt gtgctgggcg ttattgcgct gaaagatatc gtcaaaggcg
     3241 gtattaaaga gcgcttcgcc cagctgcgca aaatgggcat taaaacggtg atgattaccg
     3301 gcgataaccg tctgactgcc gccgccattg ctgcggaagc gggtgtcgat gattttctcg
     3361 ccgaagcgac accggaggcc aagctggcgt taattcgtca gtatcaggcg gaaggccgtt
     3421 tggtggcgat gaccggcgac ggcaccaatg atgctccggc gctggcgcag gcagatgtcg
     3481 cggtagcgat gaactccggt actcaggcgg cgaaagaagc gggcaatatg gtggatctcg
     3541 actctaaccc gaccaagttg atcgaggtgg tgcatattgg caaacagatg ctgatgaccc
     3601 gtggctcgct gaccaccttc agcattgcca acgatgtggc gaaatacttc gccattattc
     3661 cggcggcgtt cgcggcaacg tatccgcagt taaatgcgct gaacatcatg cgcctgcatt
     3721 cgcccgactc cgccattctc agtgcggtca ttttcaacgc cttgattatc gtctttttga
     3781 ttcccctggc gttaaaaggc gtgagttata aaccgcttac cgcttctgcc atgttgcgcc
     3841 gtaacttatg gatttacggt ctgggtgggc tgctggtgcc gtttattggt atcaaagtca
     3901 ttgatttact gctgaccgtt tgcggtctgg tgtgaggttt accatgcgtg gattacgtcc
     3961 ggcattatca acatttctct ttttgttact gattactggc ggtgtttacc cgctgctgac
     4021 caccgcgctg gggcaatggt ggtttccctg gcaggccaac ggttcgttga ttcgcgaagg
     4081 tgatacggtg cgcggctcgg cattgatcgg gcagaatttt actggcaacg gctattttca
     4141 tggtcgcccg tcggcaacgg cagaaatgcc ctataatcca caggcttctg gcgggagcaa
     4201 tctggcggtt agtaacccgg agctggataa acaaatcgct gcacgcgttg ccgcattacg
     4261 ggccgctaac ccggatgcca gcgcgaacgt tccggttgaa ctggtgacgg cgtcggcaag
     4321 cgggctggac aataacatca ccccgcaagc ggcggcctgg cagatcccac gcgtggcgaa
     4381 agcgcgtaat ctcagcgttg aacagctcac gcagctgatc gcaaaatata gccaacaacc
     4441 gctggtgaaa tatatcggcc agccggtcgt caacattgtc gaactcaatc tggcgctgga
     4501 taagcttgat gaataacgaa cccttacgtc ccgaccccga tcgtctgctg gaacaaactg
     4561 ccgcgccgca tcgggggaag ctgaaagttt tcttcggtgc ctgcgcaggc gtcggcaaga
     4621 cctgggcaat gctggcagaa gcccagcgac tgcgggcgca agggctggat attgtggttg
     4681 gcgtggtaga aacccacggg cggaaagata ccgccgcaat gctggaaggg ctggctgttc
     4741 tgccgttaaa acgccaggcg taccgtgggc ggcacatcag cgagtttgat ctcgatgccg
     4801 ccctcgcccg ccgtccggca ctgatcttaa tggacgaact ggcgcacagt aatgcgccag
     4861 gttctcgtca tcccaaacgc tggcaggaca tcgaagagct gctggaagct ggcattgatg
     4921 ttttcactac cgtcaacgtt caacatctgg aaagcctgaa tgatgtggtc agcggcgtca
     4981 ccggaattca ggttcgggaa accgtgcccg atcctttttt tgatgccgcc gacgacgtag
     5041 tgctggtgga cttgcccccg gacgatctgc gccagcgact gaaagaaggc aaagtctata
     5101 ttgccgggca ggcggagcgc gccattgaac attttttccg caaaggcaat ctgatcgccc
     5161 tgcgcgaact ggcactgcgc cgtactgccg atcgcgtaga tgagcaaatg cgcgcctggc
     5221 gggggcatcc tggcgaagag aaagtctggc acacgcgcga cgcgatcctt ttatgcatcg
     5281 gccataacac tggtagtgag aaactggtcc gcgcggcggc gcggctggcg tcacggctgg
     5341 gtagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta ccggagaaaa
     5401 aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg gagacggcaa
     5461 cactttctga tccagcggaa gagaaagcgg tagtgcgtta cgcccgtgag cataatctcg
     5521 gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa acgtttgctg
     5581 accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac
     5641 ccgcccgcac gattaacaac gcgccggata gccgctcttt taaagacaag tggcgggtac
     5701 agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta attgccatgc
     5761 agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctactt ggcgtggtgg
     5821 tggtggcgct attttatgga cgctggcctt cggtggttgc taccgtcatt aatgtggtga
     5881 gtttcgatct cttttttatc gccccacgcg gcacgctcgc cgtctctgat gtgcaatatc
     5941 tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat cgggaacctt actgctggcg
     6001 tgcgttatca ggcgcgggta gcccgttacc gcgaacaacg cacacggcac ttatatgaaa
     6061 tgtccaaagc tctggctgtg ggccgcagtt cgcaggatat cgccgccacc agcgaacagt
     6121 ttattgcctc cacgtttcat gcccgtagcc aggtgttgct gcctgatgac aacggtaaat
     6181 tgcagccgtt aacacatccg caaggaatga cgccgtggga cgatgccatc gcgcagtgga
     6241 gttttgataa aggtctgcct gcgggcgcgg gcaccgacac gttacccggt gtaccgtacc
     6301 agattttgcc gctaaaaagc ggcgagaaaa cctacggact ggtggtggtg gaaccgggga
     6361 atctgcgcca gttgatgatc ccggaacagc agcgcctgct ggagacgttt acgctgttag
     6421 tcgccaatgc gcttgagcgg ctgacgctga ccgccagcga agaacaggcg cggatggcaa
     6481 gcgaacgtga acagatccgc aacgctctgc tggcggcgct ttcgcatgat ttacgcacgc
     6541 cgctcacggt gctgttcggt caggcagaaa tcttaacgct cgatctggca agcgaaggat
     6601 cgccccacgc ccgccaggcc agcgagattc gtcagcatgt gctgaacacg acccgactgg
     6661 tgaataatct gctcgatatg gcgcgaattc agtccggcgg ctttaatttg aagaaagagt
     6721 ggttaacgct ggaagaagtg gtcggcagcg cgctgcaaat gctggaaccg ggtttatcgt
     6781 cgcctatcaa tctttctctg ccagaaccgc tgaccttaat ccacgttgac gggccgctct
     6841 ttgaacgggt gctgattaat ctgctggaga acgcggtgaa atacgcgggt gcgcaggccg
     6901 aaattggtat cgatgcccac gttgagggcg agaagctaca actggatgtc tgggataacg
     6961 gccccggtct tccgccaggc caggagcaga cgatatttga taagtttgct cgcgggaata
     7021 aagagtcggc ggtaccgggt gtagggcttg ggctggcaat ttgtcgggcg attgtggatg
     7081 tacacggcgg caccattacc gcgttcaacc gaccggaagg tggtgcctgt tttcgtgtta
     7141 cacttcccca gcaaaccgct cctgaacttg aagaatttca tgaggatatg tgacaaacgt
     7201 tctgattgtt gaagatgaac aggctattcg tcgctttctg cgcacggcgc tggaaggcga
     7261 cggtatgcgc gtctttgagg ccgaaacgct gcaacgcggc ttgctggaag cggctacccg
     7321 taagccagat ttgattattc tcgatctcgg cctgcccgat ggtgatggga ttgagtttat
     7381 ccgcgacctg cgccagtgga gcgcggtgcc ggtgattgtg ctttccgcac gtagcgaaga
     7441 gagcgacaaa atcgccgcgc tggatgccgg agcggatgat tatctgagta agccgtttgg
     7501 cattggcgaa ttgcaggccc gtctgcgcgt cgctttacgt cgccactccg ccaccgccgc
     7561 gcccgatccg ctggtgaaat tttccgatgt taccgtcgat ttagcagctc gtgtgatcca
     7621 tcggggcgag gaagaggtgc atctcacccc gattgagttc cgcctgctgg cagtgttgct
     7681 taataatgcg ggaaaagtgc tcacccagcg ccagctcctt aaccaggtgt gggggccaaa
     7741 cgcggtcgaa cacagtcatt atttgcgtat ttatatgggg catctgcgac aaaaactgga
     7801 acaggacccg gcccggcctc gccatttcat tactgaaacc ggtattgggt atc
//
LOCUS       DAZUCC010000212         7807 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZUCC010000212 DAZUCC010000000
VERSION     DAZUCC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.213"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..939
                     /locus_tag="ACHY09_004225"
     CDS             <1..939
                     /locus_tag="ACHY09_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS3887113.1"
                     /translation="PYLITSVRQTLEEQGAALQNLSKQLDSGQYQCVLNLIMNCKGHV
                     ILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMITPCDLLILISASGETD
                     EILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANETCPNNLAPTTSTTLTM
                     AIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQHDVPAVQLDASFKTV
                     IQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSATAAQMMTREPLTLPE
                     DTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1011..2159
                     /locus_tag="ACHY09_004226"
     CDS             1011..2159
                     /locus_tag="ACHY09_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFS3887114.1"
                     /translation="MLIKVKSAVSWMRAHLSAISLSDIQKHLVKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2183..3859
                     /locus_tag="ACHY09_004227"
     CDS             2183..3859
                     /locus_tag="ACHY09_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFS3887115.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDISADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            3869..4609
                     /gene="kdsB"
                     /locus_tag="ACHY09_004228"
     CDS             3869..4609
                     /gene="kdsB"
                     /locus_tag="ACHY09_004228"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFS3887116.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4606..6633
                     /locus_tag="ACHY09_004229"
     CDS             4606..6633
                     /locus_tag="ACHY09_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFS3887117.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLSVNGAPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISKAREAMHTIVTGDLSKYNLAPAFVADESECSDIVLVVDQTFND
                     MSVTYGNAGQNEFSAMLEAAMAENPQATIWVKVHPDVLKGKKTGYFADLRATQRVRLI
                     TENISPQSLLRHISRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSVTLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLLRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLRTATNRLSYSRRCTAATACVVWGVKGEQQWQVEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPSLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKGKKVILVPGQVEDDSSIKTGTISIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAELIAELADYQALDADIIQCIQHADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTFDEHHCGRRERELTLADLLYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTSRKPMFITRKKAGWVIRYYRKLIMFCKVRFG"
     gene            6668..>7807
                     /locus_tag="ACHY09_004230"
     CDS             6668..>7807
                     /locus_tag="ACHY09_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFS3887118.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDCFYCRHRHYQAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKYWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRSYWRKQLYKVTQRKVLPR
                     LMNELDQLYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGGRVIYVHDLPMPGLLRHAKAVVTINSTVGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYILM"
BASE COUNT         1996 a         1870 c         2033 g         1908 t
ORIGIN      
        1 ccatatttaa ttacttctgt tcgtcagacc ttggaagaac aaggtgcagc attacaaaac
       61 ttgtctaaac aactggattc cgggcagtac cagtgtgtcc ttaatttgat aatgaactgt
      121 aaagggcacg ttattctttc gggaatgggt aaatcagggc atgtcggtcg taaaatgtca
      181 gcgacgctgg cctctaccgg taccccgagt ttctttattc atcctgcaga agcgttccat
      241 ggcgatctgg gtatgattac gccctgcgat cttttgatcc ttatttctgc cagcggtgaa
      301 acggatgaaa tcctcaagct agttccttca ctgaaaaatt tcggcaaccg aattatcgcc
      361 attaccaata atggaaattc cacgctggcg aaaaatgctg atgccgtgct ggaactccat
      421 atggcgaatg aaacctgccc gaataatctt gcaccaacaa cgtctaccac cctgaccatg
      481 gcgatcggcg atgcgctggc gattgccatg atccaccaac gcaaatttat gccgaatgat
      541 tttgcgcgct atcacccggg cggttcatta ggtcgtcgcc tgctgacccg cgttgctgat
      601 gtcatgcagc atgatgttcc tgcggtacag ctggatgcgt catttaaaac cgtgattcaa
      661 cgtatcacca gcggatgcca gggaatggtg atggtagaag acgcagaagg tgggctggcg
      721 ggcattataa ccgacggtga cctgcgtcgc tttatggaaa aagaggattc tctgacatcc
      781 gctacggctg cgcagatgat gacacgtgaa ccgctgacgc taccggaaga caccatgatc
      841 attgaagcgg aagaaaaaat gcaaaagcac cgcgtctcaa cgttattggt gaccaacaag
      901 gcaaataaag tcactggcct tgtgcgcatt ttcgactaat taagcaacgg ggtttgagag
      961 gttagtctct cattgagttt gtgccccact ggaactttga taatcgttac atgttgataa
     1021 aagtgaagtc tgccgtatcc tggatgcgtg ctcatctgtc tgccatctca ctgtcagata
     1081 tccaaaaaca cctggtgaaa atcatcattc tggcaccgat ggcggtgctg ctgatctatc
     1141 tggctatctt cagccagcct cgctatatga gcgagtcgaa agtcgccatt aaacgctcgg
     1201 atgatttaaa cagcggcagc ctgaattttg gtctgcttct gggtgcctct aaccccagtt
     1261 ccgcagaaga tgcgttgtat ctgaaagagt acatcaactc gccggatatg ctggcggcgc
     1321 tggataaaca actaaatttt cgtgaagcgt tcagccacag cgggctcgat tttcttaatc
     1381 atcttagcaa ggatgaaacc gcagaaggct tcctgaagta ctacaaggac cgtatcaacg
     1441 tctcgtatga cgataaaacc ggattactga atattcagac gcagggcttt agcccggagt
     1501 ttgcgcttaa gtttaaccag accgtgctga aagagtcaga gcgctttatc aatgagatgt
     1561 cacatcgcat cgcgcgtgac cagcttgcct ttgcagaaac ggagatggaa aaggcacgcc
     1621 agcgtctgga cgccagcaaa gcggaattgc tctcttatca ggacaacaac aacgttctgg
     1681 atccacaggc acaggcacag gcggcgagca cgttagtcaa tacgctgatg ggccagaaga
     1741 tccagatgga agcggatctg cgtaacttgc tgacgtatct gcgtgaggac gccccgcaag
     1801 tggtgagtgc gcgtaatgcg attcagtcat tacaggcaca aatcgatgaa gaaaaaagca
     1861 aaatcactgc accacagggt gacaagctaa accgtatggc ggtggatttt gaagaaatca
     1921 aatcaaaagt tgagttcaac accgagctct acaaactgac cctgacctcc attgaaaaga
     1981 cccgtgtaga agcagctcgt aagctcaagg tgctgtcagt gatcagttcg ccacagttgc
     2041 cgcaggaatc gtcttttcca aatatccctt atttgatcgc ctgctggtta ctggtgtgct
     2101 gcctgctgtt cggcaccctg aaactgttgc tggctgtcat tgaagatcac cgagactaac
     2161 gctgtcgctg aatgagtttg tgatgaaatt atttaaatca attttactga ttgccgcctg
     2221 tcacgcggcg caggccagcg cggccattga tattagcgct gacccaaacc tgacaggagc
     2281 cgcgccgctt accggtattc tgaacggaca gaagtcggat acgcaaaaca tgagcggctt
     2341 cgacaatacc ccaccgcccg caccgccggt ggtaatgagc cgtatgtttg gtgctcaact
     2401 tttcaacggc accagcgcgg atagcggtgc gacggtagga ttcaaccctg actatattct
     2461 gaatccgggt gatagcattc aggttcgctt gtggggtgcg ttcacctttg atggtgcgtt
     2521 acaggttgat cccaaaggta atattttcct gccgaacgtt ggtccggtga aagttgctgg
     2581 cgtcagtaat agtcagctaa atgccctggt cacatccaaa gtgaaggaag tataccagtc
     2641 caacgtcaac gtctacgcct ccttattaca ggcgcagcca gtaaaagtgt acgtgaccgg
     2701 atttgtgcgt aatcctggtc tgtatggcgg tgtgacgtct gattcgttac tcaattatct
     2761 gatcaaggct ggcggcgttg atccagagcg cggaagttac gttgatattg tggtcaagcg
     2821 cggtaaccgc gtgcgctcca acgtcaacct gtacgacttc ctgctgaacg gcaaactggg
     2881 gctttcgcag ttcgccgatg gtgacaccat catcgtcggg ccgcgtcagc atactttcag
     2941 cgttcagggc gatgtcttta acagctacga ctttgagttc cgcgaaagca gcattcccgt
     3001 aacggaagct ttaagctggg cgcgccctaa gcctggcgcg actcacatta cgattatgcg
     3061 taaacagggg ctgcaaaaac gcagcgaata ctatccgatc agttctgcgc caggtcgtat
     3121 gttgcaaaat ggcgatacct taatcgtgag cactgaccgc tatgccggta ccattcaggt
     3181 tcgggttgaa ggcgcgcact ccggtgaaca tgccatggta ctgccttatg gttccactat
     3241 gcgtgcggtt ctggaaaaag tccgcccgaa cagcatgtcg cagatgaacg cggttcagct
     3301 ttatcgccca tcagtggctc agcgtcagaa agagatgctg aatctctcgc tgcaaaaact
     3361 ggaggaagca tcactttctg cccagtcctc caccaaagaa gaagccagcc tgcgaatgca
     3421 ggaagcacaa ctgatcagcc gctttgtggc gaaagcacgc accgtggttc cgaaaggtga
     3481 agtgatcctc aacgaatcca atattgattc tgttctgctt gaagatggcg acgtcatcaa
     3541 tattccggag aaaacatcgc tggttatggt tcatggcgaa gtgctgttcc cgaacgcggt
     3601 gagctggcag aagggtatga ccaccgagga ttacatcgag aaatgtggtg gcctgacgca
     3661 aaaatcgggt aacgccagaa ttatcgtcat tcgtcagaac ggtgctgccg tcaacgcgga
     3721 agacgtggat tcactcaagc cgggcgatga gattatggtt ctgccgaaat atgaatcgaa
     3781 aaatattgaa gttacccgtg gtatttccac aatcctctat cagctggcgg tgggtgcaaa
     3841 agtgattctg tctttgtaag gagttgagat gagcaaagca gttattgtca tcccggctcg
     3901 ctatggctcc tcgcgcctgc cgggtaagcc actgctcgat attgttggta aaccgatgat
     3961 ccagcatgtt tacgagcgtg cgttacaggt ggcgggcgtc gcagaagttt gggtggcaac
     4021 agacgatcag cgtgttgaaa aggccgtgca ggcatttggc gggaaagcca tcatgacgcg
     4081 caacgatcat gaatccggca ccgatcggct ggtcgaggtg atgcataaag tcgaggcaga
     4141 tatctacatt aacctgcagg gcgacgaacc aatgattcgc ccgcgggatg tagaaacgct
     4201 gttacaagga atgcgtgatg accccgcgtt gccagtggca acgctatgcc acgcgatttc
     4261 tgccgaagaa gcggctgagc caagcacggt aaaagtggtg gtaaataccc accaggatgc
     4321 gctttatttc agccgttcgc cgattccgta tccgcgtaat gctgaaaaag cgcgctacct
     4381 gaaacacgtt ggtatttacg cttatcgtcg cgatgtgctg caaaactaca gccagttacc
     4441 ggagtccatg ccagagcagg cagaatcact ggagcagctg cggttaatga gcgcggggat
     4501 caacatccgc acatttgagg ttgccgcaac cggtccgggc gtcgacaccc cagcatgcct
     4561 ggaaaaagtg cgcgccctga tggcacagga actggctgaa aacgcatgat tggcattttc
     4621 tcgtccggta tctggcgtat tccgcatctg gagaaatttc tggcgcaacc gtgccagaaa
     4681 ctttctctgc tgcgccctgt tccgcaagaa gttgatgcta tcgccgtgtg gggacatcgt
     4741 cccagcgcgg caaaaccggt cgccatcgcc aaagcagcgg gaaaacccgt catccgtctg
     4801 gaagatggat ttgtgcgttc gctggatctt agcgtcaatg gtgcgccgcc gctttctctc
     4861 gtggtggatg attgtggcat ttactacgat gccagcaagc cttcagcact ggagaaactg
     4921 gtacaggata aagccggaaa tacagctctg ataagtaagg ccagagaggc gatgcacacc
     4981 atcgtgaccg gggatttgtc gaaatataac ctggcgcctg cgtttgtggc tgatgagtca
     5041 gaatgttcag acatcgttct ggttgtcgat cagacattta atgatatgtc agtgacgtat
     5101 ggtaatgctg gtcagaatga gttttctgcc atgctggagg ccgcgatggc ggaaaatcct
     5161 caagcgacaa tttgggtgaa ggtgcaccca gatgtactga aaggaaagaa aacaggttat
     5221 ttcgccgatc tgcgcgccac gcaacgagta cgtttgatta cggagaatat cagtccgcag
     5281 tcgctgttgc gacatatttc cagagtttac gtcgtgacct cccaatacgg ctttgaggcc
     5341 ttactggcag gaaaaccagt gacatgcttc ggtcaaccct ggtatgcagg ctggggctta
     5401 acggacgatc gtcatccgca gtcggctttg ttatctgccc gacgcggttc tgtcacgctg
     5461 gaagaacttt ttgccgcagc atacctgcgt tactgtcgct atatcgaccc actaacgggg
     5521 gcagcaagtg acctgtttac cgtactgcaa tggctgcaat tactgcgcaa ccatcagcaa
     5581 cagcgtaatg gctatttatg ggcgccaggc ttaacgctgt ggaagtcagc aatcctgaaa
     5641 cctttcttgc gtaccgcgac aaacaggctg agttattcac gtcgctgtac tgctgccacc
     5701 gcctgcgtgg tatggggtgt aaaaggagaa cagcaatggc aggtcgaagc gcagcgaaaa
     5761 tcactgccat tatggcgaat ggaagatggt tttctgcgtt catccggact tggctcggac
     5821 ctgctgccat cgctatcgct ggtgctggat aaacgcggaa tttactatga cgccacgcgc
     5881 cccagcgacc tggaagtgct gcttaatcac agccagttaa cgctggcgca gcagatgcga
     5941 gctgagaagt tgcggcaacg actggttgaa agcaaactga gtaagtacaa cctgggagcc
     6001 gatttctctc tgcctgccga agccaaaggt aaaaaagtta tcctggtgcc gggtcaggta
     6061 gaggacgatt cctctattaa aacaggcact atctcaatta agagcaacct tgagttatta
     6121 cgcacagtac gcgagcgtaa tccgcacgcc tacattattt ataaaccgca cccggatgta
     6181 ctggtgggga atcgcaaggg cgatattccg gcagaactga ttgctgaact tgctgattat
     6241 caggcactgg acgcagatat tattcaatgc attcagcacg cagatgaagt gcataccatg
     6301 acgtcgctgt cggggtttga agcgttatta catggtaagc acgtacattg ttacggcctg
     6361 cccttctatg ctggttgggg tttaaccttc gatgaacatc actgcgggcg tcgtgagcga
     6421 gaattaacgt tagcggattt gctatatcag gcgctgattg tttatccaac ttatatccac
     6481 ccaacacggc tacaacctat tactgttgaa gaggcagcgg aatatttgat ccagacgtcg
     6541 cgcaagccga tgtttattac ccgaaaaaaa gcgggatggg taatacgcta ttaccgcaaa
     6601 ttaattatgt tctgcaaggt tagatttggc taaacaattt catagttgac tattacgcaa
     6661 attatttatg caaggtaatg cactaaccgt tttattatcc ggtaaaaaat atctgctatt
     6721 gcagggaccg atgggaccct ttttcagtga tgtagccgag tggctggagt cattaggccg
     6781 taacgctgtg aatgttgtat tcaatggagg ggattgtttt tactgtcgcc accgacatta
     6841 tcaggcttac taccagacac cgaaagagtt tcccggttgg ttacgggatc tccaccggca
     6901 atatgacttt gataccatcc tctgctttgg tgactgccgc ccattacaca aggaagcaaa
     6961 atattgggca aagtcgaaag gtatccgctt tctggcattt gaggaagggt atttacgccc
     7021 gcagtttatt accgttgaag aaggcggagt taacgcatat tcatcgctac cgcgcgatcc
     7081 ggatttttat cgtaagttac cagatatgcc tgcgccgcac gttgagaact taaaaccttc
     7141 aacgatgaaa cgtataggtc atgcgatgtg gtattacctg atgggctggc attaccgcca
     7201 tgagttccct cgctaccgcc accataaatc gttttccccc tggtatgaag cacgttgctg
     7261 ggttcgttca tactggcgca agcaacttta caaggtaaca cagcgtaagg tattgccgag
     7321 gttaatgaac gagttggacc aactttatta tcttgccgtt ttgcaggtat ataacgatag
     7381 ccagattcgt aaccacagta attataacga tgtgcgtgac tatattaatg aagtcatgta
     7441 ctcattttca cgtaaagcgc cgaaagaaag ttatttggtg atcaaacatc atccgatgga
     7501 tcgtggtcac agactctatc gaccattaat taagcggttg agtaaggaat atggcttagg
     7561 tgggagagtc atttatgtgc acgatctccc gatgccggga ctattacgcc atgcaaaagc
     7621 ggtggtgaca attaacagta cggtgggaat ctctgcactg attcataaca aaccacttaa
     7681 agtgatgggc aatgccctct acgacatcaa gggcttgaca tatcaagggc atttacacca
     7741 gttctggcag gctgatttta aaccagatat gaaactgttt aagaagtttc gtggatatat
     7801 attgatg
//
LOCUS       DAZUCC010000213         7802 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZUCC010000213 DAZUCC010000000
VERSION     DAZUCC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.214"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..237
                     /locus_tag="ACHY09_004231"
     CDS             <1..237
                     /locus_tag="ACHY09_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFS3887119.1"
                     /translation="VSFDWEGDALELLNELARLRGQQFSYSGVRLPLPVSLHVRDMTF
                     ADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            234..1070
                     /locus_tag="ACHY09_004232"
     CDS             234..1070
                     /locus_tag="ACHY09_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFS3887120.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            1054..2202
                     /gene="traJ"
                     /locus_tag="ACHY09_004233"
     CDS             1054..2202
                     /gene="traJ"
                     /locus_tag="ACHY09_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFS3887121.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            2199..2489
                     /locus_tag="ACHY09_004234"
     CDS             2199..2489
                     /locus_tag="ACHY09_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS3887122.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            2553..6569
                     /locus_tag="ACHY09_004235"
     CDS             2553..6569
                     /locus_tag="ACHY09_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFS3887123.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAE
                     AGTDLSLLKRWLPRPQEMSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAQSGAYKAYLDGRPSGWYRDYRSADNSPTTWTFSRAENIDPLARLHLKASAQQNR
                     DEKERKLQQQYNKQAGYARSYLNGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKGSEKSGNWFAFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTIDADNMINVAKNARKIWTDSPFVFCADNDHQRKINKGVSSAKKAAASTH
                     GEVIIPTFTEAEKAQGLTDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREA
                     IVIGNLIFTPVKDEKPQVSPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAY
                     ASLQSVTAGTASPNEPQVNAVSAIDSNAKNTSAATTDDRNVLAQETNADATVTTTAHD
                     TGTAETDSPSEPAEIQPVQQASPEPSAKDKPQENVVSATDNDAENTSSTTTALRSYDT
                     PETSVSDATGTQHTATTQPEEPQQVSMGSADDWIAFAEDLNADAPGNATVHSTDNAET
                     KTLSEPAGMHPVQQATPEPTEENKPQEEGVMSATDTGEESAAAAIDDDLNTVAEDLNA
                     DVTVMTTALHTDTAETASPSEPAEIQPVQQATPEPTAEDKPKEERPVSATETSTENEA
                     ARPETPSLFSRLRRFLTGGKSRTAEPPVIQENTLTEAETATEAAAPPSPPETSPEDTS
                     SAEPDTIIFAPRRPNSPQRHNLDEIIKNLTYDELPDNTALCRVDGEPAFLDKLNRLEM
                     VDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPVQRA
                     ALEKLRQEKGIAHDAIVTHRPTPELNRLSPEETTMAQPAPPVSPKAETEQTAGSTAET
                     PHPEAPVTAKAEAPRAGTPEQPAASSSAPEKAPVSAQPQQPDTPEEEPPGKLRPGESV
                     TAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVKNHQKGNPVTLTLQAR
                     ETFRLSGETRDRIRNIWAMTPVSNGITVDNDHPHAGQHILEFPMKTFSQVMSQMQQHW
                     PQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAPNELIPAIASADKDNR
                     QLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINALTDNGLQFAGYGEAVN
                     HDENTHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKA
                     EDRQEKTLQQAQSHSPGL"
     gene            6586..6936
                     /locus_tag="ACHY09_004236"
     CDS             6586..6936
                     /locus_tag="ACHY09_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS3887124.1"
                     /translation="MNMKKTLLVILTSISLASPAAVAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            6948..7643
                     /locus_tag="ACHY09_004237"
     CDS             6948..7643
                     /locus_tag="ACHY09_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFS3887125.1"
                     /translation="MSEAHPTSPVSPETDNSGDIYKQTLSTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            7654..>7802
                     /locus_tag="ACHY09_004238"
     CDS             7654..>7802
                     /locus_tag="ACHY09_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFS3887126.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQAPGNA
                     PAGGPP"
BASE COUNT         2005 a         2325 c         1986 g         1486 t
ORIGIN      
        1 gtttcctttg actgggaagg cgatgccctg gaattgctca acgaactggc ccgtctgcgc
       61 ggccagcagt tcagctacag cggcgtccgt ctccccttac ccgtctcact gcacgtccgc
      121 gacatgacct ttgctgatgt ccttcgtctc actgaagccc agactgcctg gcgggcaaca
      181 ctctcgcagt cagccgggcg tttacatctg tcttttatga cgccggagaa aaaatgaaaa
      241 cacacctgtg tctcctgctg tctgctctcc tgcttacagg ctgtgccgga cagcatcacc
      301 gttcttctgc cgtcatgaca gaaaacgcac cgcccccgga tatccgtgca tggctgacgc
      361 cctccggaaa ccaggatgat ggtatttcag atacccgtcg tcagatgctc acggatgccg
      421 gcagaacgct gggattccgt ggaggaaaag cacagcgctc atgggaactg attcaggcac
      481 tgaatgcccg ggaaagtacc ctgaatgccc tgtatgattt ccggcctctc atcagcaagg
      541 aaggctggct gcctcctgtt atcgatgaag cgcaggatgt tgcacacatc agaccggacc
      601 agataagaac agcctcccgt gtctggacca tcctccgccc ggaacgcttt gtcagtaatc
      661 cacctggatg gcgtgactgg ctgctgaaag gactctcaac caccgccaca ccaggcactg
      721 aaggccgggt tgtgccggaa gaccgggcac aacgcagact atgggaaaac gccttacgtc
      781 agggatggca ggaagggcgc ctcaacgcag atctcacact ggaagctaat cagaagcgcc
      841 tgacccgtga ttaccggggc atgatgcttt acgcccttct gtggcgtcag ggcatgatca
      901 cacgtccaga tgtcacagag cagatgcaga cggttaccgg caacgggcaa aaactcatca
      961 ctggcgatcg cgtccgccgc ctgaaaacac atgctgaatt tacgctgcag aaatcgcgct
     1021 ggcgccctct tgtcagcacc gaaggagaga aaaatgatat caccagatga cttcggtacc
     1081 ttccccttct cccgttttac ggcagaagag tttcgccatt tttttgcctg gtgtgcccgc
     1141 caccgcgtca gtgatattga tctgaccggc ggttcaccgg tatccgtcag tcgctttgga
     1201 cggcgggttc gctcatccgt aaatcccctt ccgtcctcca tcatgatggc actgagtgat
     1261 gagctgtttg ggcgggaggt tcttccccgg gttctgtcag gtcatcccgt ggatcgtacc
     1321 attcaggtca gcggagatgc ctccggtcgc tatggcctgc agcgaggtca gcgtgttcgc
     1381 ctgcgctgcc acctgataca gggaacatcg gcaacagaag aaaaagccat ttccgtcacc
     1441 atgcgggtga tcccgtcaga tattccggac atcctcacta tgaatattga accggatctg
     1501 ctggatgcca tgctcagaaa aaccggcctg ggcttcatct gcggagaaac cggctccggg
     1561 aaatccacac tggcggcggc actgtatcgc cacattctga aaaatttccc cgatcgcaaa
     1621 atagtgacct atgaagatcc ggtggaatac attctgggcg gagagaatga ccttctgcct
     1681 ccccatcagg ccgaaatcgg gcgcgatgtg gaaagttttg ctgcaggact tcgctctgcc
     1741 gttcgccgta atccggacat cattggtatc ggtgaaatca gggattatga aacagcagat
     1801 gccgcagtcc aggcgggtaa taccggacac ttttgtatcg gcaccatgca cacaaaatca
     1861 cccggagaaa cctttgcccg cctgctggga ttgtttcctc ctgaaatcag ggattcgatg
     1921 gctgcagcca cgctttccct cgtacagttc attctggtgc aggtactggt cagaacaaat
     1981 gacggcggca ggcaggctgt ccgggaatac atcgtcatga ctgacgaact gcgcaatacg
     2041 ctctccggac aatcccatgc tacatgggga cattacattg acgaaatcat tcgtcaggaa
     2101 aaacggcgga taagggatca ggttctgacc atgtatcaga acgaccgcat tgagaaatca
     2161 gaagcagcgc tcttcatccc ggccggagag ttcccccgat gaaacgcgaa ggcttctggc
     2221 gggatgtaac accaccgctc aacatttatg gtgtccccct gccgtttacc atgatttacc
     2281 tgctgttgtt tccgtatccg tcaaaaacga cattctgggt gtgcagcggc attctgctgt
     2341 tttttatgct cctgggatat tccggctgga cagtccgcac cctctgtata cgtctgtaca
     2401 gcctgcttca gggtaagcag gcatcgggac gtccctggtg gtaccgacac cgcacagaat
     2461 taccccgtga atggcacgga ccagaatagc cctcttttgt ccatcatccc ggcgtatcac
     2521 aacgccctca tatttaatcc ccggagaacg acatgaagtc cagatatcgt acctggcttg
     2581 ctgtcccgcc tgaggagaca gaagccgtaa aaaatgcagt ccccccgtta aatggccgca
     2641 aagcggtagc atgggatcca gagaaaaaac tctggtatgc cgaggcgggt accgaccttt
     2701 cactgctgaa gcgctggtta ccccgtccac aggaaatgtc gatggatgcc ggcgatccgg
     2761 tcacagaatt tgctcaggtc ctggaaaacg ccgggctggt tattcaggga ttgccacaga
     2821 tggatggcgc catacacaga gtcgccaccc gggatgacaa aaaaggggcc caaagtggcg
     2881 catacaaggc ctatcttgat ggccgcccct ccggctggta ccgcgattac cggagcgctg
     2941 ataacagtcc cacaacctgg acattctccc gcgcagaaaa tatcgatcct ctcgccagac
     3001 tgcatctgaa ggcatccgct cagcaaaatc gcgatgagaa agaacgtaaa ctacagcaac
     3061 agtacaacaa acaggccggc tatgcccgct cctatcttaa cgggttgccc caggcaacag
     3121 cacatgaata cctgacacgc aagggcatcc gggccgcgcc tggcgtgcgg ctgaacaata
     3181 aaaatgagct ggttattccc ttcagcaacg gccgggggga aatacgttcg taccagcgta
     3241 ttccggtcac cggtgggaag gatgcccgca ttctgaaagg ctctgagaaa agcggcaact
     3301 ggttcgcctt cggaaccccg gaaaatggcc gtcccctgtt atttgcggaa ggttacgcta
     3361 ctgccgcctc cctgcacgaa gcaaccggcc ttcctgttct gatgacaatt gatgccgaca
     3421 atatgattaa tgtggcaaaa aatgcccgca aaatctggac tgacagtccc tttgtcttct
     3481 gtgccgacaa cgaccatcaa cggaaaatta ataaaggtgt ttcaagtgca aaaaaagctg
     3541 ccgcaagcac gcatggcgaa gtcattattc cgacctttac ggaggcggaa aaagcacagg
     3601 gactgaccga tttcaatgac ctggacgcca gccggggacg agacgccttt cagcgagcca
     3661 tgaacgcaca gttagaatat atcggtatcc ccacgcccaa caatgacact actgaccgcc
     3721 gtgaagctat cgtgatcggc aacctgatat tcacaccggt aaaagatgag aagccacaag
     3781 tgtcccctga aaacagacag agcacagccc ctgaaccggc agtgtcggaa cagaatcatc
     3841 cttatgacat ccctgcagaa gagacagaca atcaggcata tgaagatgac tatgcagcat
     3901 atgcatccct gcagtcggta actgcaggga cagcctcccc gaatgaaccg caggtgaatg
     3961 ctgtttctgc cattgacagc aacgcaaaga atacctctgc cgccaccact gacgaccgaa
     4021 atgtattagc acaagaaacc aatgctgacg ccacggttac gaccacggct cacgacaccg
     4081 gcacagcaga aacagattca ccttctgaac ctgcagagat tcagccagtg cagcaggcca
     4141 gtcctgaacc atcggctaaa gataaaccac aggagaacgt agtgtctgct actgataatg
     4201 acgcagagaa cacctcttcc accaccactg ccctccggag ttacgacaca ccggaaacat
     4261 ccgtctccga cgcaaccggg acacagcaca ccgccacaac gcaacctgaa gaaccacagc
     4321 aggtctccat gggcagcgct gacgactgga ttgcatttgc ggaagacctc aatgctgatg
     4381 ctccggggaa tgccactgtt cacagcaccg acaacgcgga gacaaagaca ctttctgaac
     4441 ctgccgggat gcaccctgtt caacaggcaa cccctgaacc aacggaagaa aataaacctc
     4501 aggaagaggg cgttatgtct gccactgata caggtgaaga gagtgccgct gccgctatcg
     4561 atgacgacct gaacacagtt gcggaagacc tcaatgctga tgttactgtg atgaccacag
     4621 ctctccacac cgatacagca gaaacagcct caccttctga accagcagaa attcagcccg
     4681 tacagcaggc aacccctgag ccaacagcag aagataaacc gaaggaagag cgtcctgtgt
     4741 ctgccactga gaccagcaca gagaatgaag cagcccgccc tgaaacacca tcgcttttca
     4801 gccgcctgcg ccgttttctg accggtggaa aatcacgtac cgccgaacct cccgttattc
     4861 aggagaacac gcttacggag gccgaaactg cgacagaggc cgcagcacca ccgtcaccac
     4921 ctgaaaccag cccggaagac acatcgtcag cagaaccgga cacgattatc tttgcgcccc
     4981 gccggcccaa cagtccgcag cggcacaacc tggacgagat tattaaaaat cttacatacg
     5041 atgagttacc cgacaatact gcgctctgcc gcgtcgacgg cgaaccggca ttcctggata
     5101 aactcaaccg tctggaaatg gtggatggtg ccagtgctga tgacaaaaag gtcctggctg
     5161 cccttgctgt ggccaccaac ttctatggcg gcgttatcga gctgaccggc agtgatgcct
     5221 ttaaacaaaa agccatgcag ctgattgtcg aatatgacat caaggttcgc atgaaactcc
     5281 cggtccagag ggctgcgctg gaaaaactcc gccaggagaa agggatagcg catgatgcca
     5341 tcgtcaccca cagacccacg ccggaactca accgcctgtc accggaagag acaacaatgg
     5401 cacaaccggc cccccctgtg tcaccgaaag cagaaacaga gcaaactgca ggcagtacgg
     5461 ctgaaacacc gcatccggaa gcgccggtca cggcaaaagc tgaagccccc cgagccggca
     5521 caccggaaca acccgccgcc tcgtcgtctg cgccagaaaa agcccctgtc agtgcacagc
     5581 cgcagcaacc cgacacaccg gaggaagagc cgcccggcaa gctcagaccc ggtgaaagtg
     5641 tgactgccgt cctgacaaac tacggagaaa aagagtatgc gcccggaaaa gggcgctgct
     5701 tttttgtgga gctcaccaac cgcagtggca aacgggaata ctggggaaac ggcctggaag
     5761 aactggtcaa aaaccaccag aaaggcaacc ccgtcacact gacgctgcag gcgagagaaa
     5821 cattccggct gtccggtgaa acacgggatc gcatccgtaa tatctgggcc atgactcctg
     5881 tcagtaacgg cattaccgtc gataatgacc atcctcatgc tggtcagcat atcctggaat
     5941 ttccgatgaa aacattcagt caggtaatgt cacaaatgca acaacactgg ccacagataa
     6001 tgcaggaagt tcgctccccc aaaaatcctc ccaataatct gtacattggt gaagacaggc
     6061 aacctgcatc agcacctcag gatcaatcaa tgcgactccc cctcgctggt aatgccccca
     6121 atgaactgat accagccatc gcctctgccg ataaggacaa tcgtcagttg aatcttctgc
     6181 tggtccacag cgcagatgat catctgcagg gcgtggtccg cctgaacggc accctttacc
     6241 cggcactggc caccccgtca gctgacaacc gtcagctggt catcaatgcc ctgacggata
     6301 acggtctgca gtttgccggt tacggtgaag ctgttaacca tgatgagaat actcatcaac
     6361 gtccgtcccc acaaataatg cagtttcacc tgaagcaaca ggactccccg ttgtttgccg
     6421 ccatccacaa accggaagag caaccggaca agcttttccg ctctctgggt tttgagcaga
     6481 cctggaaaga gtggtctgac agccaaaagg cggaggacag gcaggaaaaa actctgcaac
     6541 aggcacaatc ccacagcccc ggcctgtgag ccggtaagga cgaccatgaa catgaaaaaa
     6601 acgctgctgg tcatactgac cagcatctcg ctggcttctc cggctgccgt ggcagcaacc
     6661 tgtcgggcag gtgctgccgc agaagccggc agcaaagccg cctgggagcg ggcgcgcaca
     6721 gccagccagg catggtcaca gcgagaaaac gacgtcgcct cctccctgca gtcctgcctg
     6781 tcccgcatta aggacgtcag catcactctt ccacaattcc ccagcctgga caaaatcctt
     6841 cgcgatctgg aaaaccaggt ctgtgatgcc gtcgtggatc gggtgaatga acacctgccg
     6901 gggaatatcg acccctggaa agactataac ctctgaacag gagccccatg agcgaagcac
     6961 accccacatc ccctgtctct ccggaaacag ataacagcgg agatatttat aaacaaacgc
     7021 tcagtaccat tgcacagtca gaacaacggg ccgcactggt tcccggactg gtcagggcct
     7081 tgctgtgtag cagcgcagct ctcgtcatca gtctgaccgg caatatcata cagtactggc
     7141 ataacagcaa cgtaacgcgt gaatactttg ccaccgacaa tggccggctg gtccgcctgg
     7201 cccccaccag tcagccagcc tggagtcaga gcgacgtcat gacgtttggc agcatgacgc
     7261 tgagcgaagc ttttaatctg gattttgtcc actacaggaa acaaatcacg acactggccc
     7321 cccgcttttc cgaagccggt tttgccggct atgtgaatgc tcttcaggcc tccaacattc
     7381 tggacaccat taaaaaggag aaatttaacc tcacggccac catcggggcc ggcacgctgg
     7441 tgaatcaggg ccagatggaa aacggcatct ggttctggaa attccagtat ccggtcagga
     7501 tgcgtctggt cggtcagacc agcacccgcc ccgagcaggc cttcaccttt gaaatcactg
     7561 tccggcgcgt tgacccacga atcaaacccg gcggcatgga aatcagccag atggtttccc
     7621 gtaacgccgc atcaggaacc tgagactcat atcatgcaaa tacatcatct tcttggcgca
     7681 ctgcttattg tgtgcggcac tgtcacggct gcagataacc cgggctggca gaacgcacga
     7741 accccacagg ctggcggaaa gacaggacag gctcccggga acgcccccgc aggcggcccc
     7801 cc
//
LOCUS       DAZUCC010000214         7777 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZUCC010000214 DAZUCC010000000
VERSION     DAZUCC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.215"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..218
                     /gene="yiiE"
                     /locus_tag="ACHY09_004239"
     CDS             <1..218
                     /gene="yiiE"
                     /locus_tag="ACHY09_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFS3887127.1"
                     /translation="AMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQQ
                     FPVRKAKGGRQRGETVVVDDQCKEHKE"
     gene            complement(433..1362)
                     /gene="fdhE"
                     /locus_tag="ACHY09_004240"
     CDS             complement(433..1362)
                     /gene="fdhE"
                     /locus_tag="ACHY09_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFS3887128.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1359..1994)
                     /gene="fdoI"
                     /locus_tag="ACHY09_004241"
     CDS             complement(1359..1994)
                     /gene="fdoI"
                     /locus_tag="ACHY09_004241"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFS3887129.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1991..2893)
                     /gene="fdoH"
                     /locus_tag="ACHY09_004242"
     CDS             complement(1991..2893)
                     /gene="fdoH"
                     /locus_tag="ACHY09_004242"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFS3887130.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2906..5956)
                     /gene="fdnG"
                     /locus_tag="ACHY09_004243"
     CDS             complement(2906..5956)
                     /gene="fdnG"
                     /locus_tag="ACHY09_004243"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5369..5371),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFS3887131.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKVYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6150..6983
                     /gene="fdhD"
                     /locus_tag="ACHY09_004244"
     CDS             6150..6983
                     /gene="fdhD"
                     /locus_tag="ACHY09_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFS3887132.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRHDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7072..7762
                     /locus_tag="ACHY09_004245"
                     /pseudo
     CDS             7072..7762
                     /locus_tag="ACHY09_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT         1822 a         2006 c         2065 g         1884 t
ORIGIN      
        1 tggcgatgaa tacggttttt cttcatttat cagaagaggc aattaaacgg ctgaacaagc
       61 ttaggggctg gcgtaaggtt tcacgatctg caattttacg cgaagcggtt gagcaatatc
      121 tggagcggca gcaatttccg gtgcgtaaag caaagggtgg caggcaaagg ggtgagacag
      181 tagttgttga tgatcagtgt aaggagcata aggaatgaca aaaaatttat tattcttgaa
      241 tataaaaacc agatgccctt attctggtat taatacaaga ctgttttact tgaacttata
      301 ataactgtaa ctgttacatc atatctggaa aacgcctcgc aaaatacgag agattttgcg
      361 ggaatagcag gtgtcgtcac accctatgag ctgtaatccg ggcgaccagc gccacccgga
      421 aaacgtcaga gattactccc cttcacccgg gaacaggaac gggttgatgg aactgcgggc
      481 gtagccttct tgctccattc gcgcgtccag taccagagag gcgaggtcat ctgccacggc
      541 ttcaactttc ggttcttttt cctgataaag aatcttcagg taagtgccac agtcatcgca
      601 gctttcggct ttaatcgcgg cctgttcgtc atccagcgac cagtaatgca gtttgccgct
      661 ctgctcgcag ttgctgcatt ttacgcgcac cacgtgccat tcggtttcac acaggttgca
      721 atgcaggtaa cgcagaccct gagtggtgcc aatttgcacc atgctggaca ccggcataga
      781 gccacatacc gggcaatatt gacgttgttc gccgtattca gcgcgggctt tgccggggat
      841 cagattagcc atctgcgccc agtagagcga cagcgcagcc cagataaacg gcgctttatc
      901 gctgctgacg gacgagaaat cagaggcaaa cagtgcgctg gccatatctt ccagctcctg
      961 agtcgatgcc ttctccagat tctcaatcac tgccagcgcc gggccgctca tttcaggttt
     1021 cagctcagca atcagcgcca tcagcagctt ttgccagtgc ttatcacgcg gcagaacgtg
     1081 aatatccagc gggggcttgc cttgtgcgct ggcttcttta atgcgcgtgg tcagatccat
     1141 ctccagcgga tggtcgtaca gcaccacttc ctgggcgtgg gcgataagcg cagcaaagcg
     1201 caggtaatca cccagcggat tattttctgc cagctcgcgc agacgctcgg cgcggcggtt
     1261 gtataaattc ttgagccgag ggaacaataa cggcggaatc atatccgccg tacgtttctc
     1321 gctcgaaccc agctcatctt gcgggattat gcgaatactc attcagcttt cttttccgtt
     1381 gtcttgcgga cctcacggta ccagcgcggg tgatgtttct tcgcccaggc gctggtaacc
     1441 catccttcca ccatggcggt aatcgtgcct ttcacccaaa gggcggcgta gatatgcacc
     1501 atgataacca caattaacgc cactgcggca aatgaatgca gcattaacgc gaatcggatc
     1561 accgggattg agaaagcagg tgcaaaataa ggacgccaga taatcacgcc gctcaccagc
     1621 agcagaacca ggaaaataat cgccgcccag aaaacgcatt tctgaccgaa gttataacgc
     1681 ccggtgtcac ctacttcctc gttgacgacg atcttacgaa tattcttcgc ccaaaagata
     1741 tcatcccgat tgattaggtt gtgatgccag taacggaaaa acatgatgat gaacgaggca
     1801 aacataacca cgccgacaaa cgggtgcaga atgcgcgcca gctgcggtgt gcccatgatt
     1861 tgcatcaacc agttgaagga cgggaacaaa aagcccagcc cgctcaccgc cgccaggatg
     1921 aagcagaagg cggtgatcca gtggttgata cgttccggcg cggtgtagcg cacgatggtg
     1981 tcacgtcgtt tcatttgcgc tcctcgtctt tctcttcgtg cagattattc tcttcctcat
     2041 ccgcacggtt cggaccgaca cccacgtagt ggaagatact ggccgcaaag gtagccgcaa
     2101 agccaacagc cgcgagcggt ttccagatgc ctttccagaa tttcacggtt tcgctgattt
     2161 ccgggttctc cggcaagcca tgatacagat ttggcttgtc agcatggtgc agcacgtaca
     2221 taacatgtgt accaccgacg cctgccgggt cgtacaaacc cgcattgtcg taaccacggg
     2281 ttttcagctc cgccacgcgc tcgctcgcca gcgttttcat cgactctttc gtaccgaagt
     2341 gaatcgcgcc cgttgggcag gtcttcacgc aggccggttc ttgcccaacc accacgcggt
     2401 caacgcacaa cgtacatttg tagacgcggt tgtcttccgg gttgaggcgc ggaatgtcga
     2461 acggacagcc cgcaatgcaa taaccgcagc cgatgcactg ctcggactgg aagtcgacga
     2521 taccgttggc atactgaata attgcccctt ccgccgggca cgctttcagg cagcctggat
     2581 cggaacagtg catacagccg tctttgcgga tcagccattc cagtttgtcg ttctgctcca
     2641 cttccgagaa tcgcatcacc gtccacgatt tggcacttaa atcattgggg ttgtcgtaca
     2701 ccccaatgtt attgccgacg gtatcgcgaa tgtcgttcca ctctgaacac gccacctgac
     2761 aggctttaca gccgatacag gtggtaacgt cgatgagttt cgccacttct tcctggaagt
     2821 cccgcgcctg aggcgcgggg gtcagaccgt tagtcgcgga acgacgaatg atatcttgcg
     2881 attgataagc catatgtcgt ctccgttaca ccttttccac attcacaagg aaggacttaa
     2941 actccggcgt ctgcgtgttc gcatcaccga cgaatggcgt caacgtattg gcaataaagc
     3001 cttttttcgc aacaccttca tagccccagt gaatcggaat accgatggta tcgatatctt
     3061 tgccgtttgc tttcagcgtg cgaatacgtt tggtcactac cgctttggct ttgatatagc
     3121 cacggttgga ggagactttc acagtatcgc cctgggcaat gccaagttta ttcgccagcg
     3181 actccccgat ttccacaaac tgctctggtt gcaaaatcgc attcaacagc gcgtgtttgg
     3241 tccagtagtg gaagtgctcg gtcagacgat aggtggttcc gacatacggg aacttatcgg
     3301 ctttacccaa tgcttcggcg tcgtctttaa agatacgcgc agccgggttc gagataacgt
     3361 ttggatgcag cgggttagtt cccagcggcg tttcaaacgg ctcgtagtgt tccgggaacg
     3421 gaccttccgc catcttatcg agggcaaaca gacgtcccat gccttcctgc tgcatgataa
     3481 acggccctac gccgctgccc ggaggcgctg cgctgtagtc cggaatatcc cagccggtcc
     3541 acttagtgcc gtcccatttc agcaactgac gcttcggatc ccatgggtta ccctgcggat
     3601 ctgcggaggc gcggttatac agaatgcggc ggttaagcgg ccatgcccat gcccagccca
     3661 gcgtgttacc gaggccagac ggatcggcgt tatcacgacg cgccatctgg ttgccttccg
     3721 gcgtccagct accggcgaaa atccagcagc cacaggaggt tgtaccgtca tcgcgcagtt
     3781 gagcgaacga gctaagttgt tggcctttct tgacgataac cgcaccggtt gccggatcgg
     3841 taatatcggc cagcgcctta ccgttgctct ccatcgccac ttcttctgaa gatggctcat
     3901 gcggaatggc gtagttccag gtcatgttca gcacctggtc cgggtttgcg ccaccctgtt
     3961 ctgcatacat cttgcgcaag cgcaggaaga taccggagag gatctcgcca tcagtcagcg
     4021 caatccctgg ggcgtccgca cctttccagt gccactgtaa ccagcgaccg gagttaacga
     4081 ttgaaccgtt ctcttctgcg aagcaagtcg atggcagacg gaacacttcg gtctggatct
     4141 tcgacgagtc aacttcgttc agctcaccgt ggttctgcca gaagttagag gtttcagtgt
     4201 tcagcgggtc gatggtgacg aggaacttca gtttcgacag acaaccgatc actttgtttt
     4261 tgttcgggaa tgaggcaaca gggttaaagc cctggcagat atagccattg accttgcctt
     4321 ctttcatcat ctcgaagtat tgcaggacgt cgtagacttt atcccacttc ggcaaccaat
     4381 caaagcccca gctattttcc gccgtcgctt tatcaccaaa gaaggccttc atcatagaga
     4441 cgaagaattt cgggtagttg ccccagtagt ttacctggcc ttccagcagc ggttttggcg
     4501 tgttggcggt aaggtaggtt tgcagatcgg tctgcttctc gctcggcagc gtcatgtaac
     4561 ccggcaggct ctgcgacagc agccccaggt ccgtcagccc ctgaatattg gagtgaccgc
     4621 gcagggcgtt aacgccgccg cctgccatcc ccatattgcc gagcagcaac tggatcatcg
     4681 ccatcgtacg aatgttttgc gcaccaatgg agtgttgcgt ccagccgagc gcgtaaagga
     4741 acgaggcagt tttatcgtga gcactggtct cagcgatgta ttcgcagact ttcaggaatg
     4801 cgtcttttgg cgtaccacag atgttttcaa ccacatctgg cgtgtagcgg gaaacgtgct
     4861 gtttcagcaa gttccacacg caacgtggat gttgcagggt agtatcgcgt ttggcgaagc
     4921 cgttttcgtc cagttcgtaa gtccaggagg atttatcgta cttacgtttt tccgcgtcat
     4981 agccggtgaa caggccatct tcaaagccgt aatcctcacg cacgatcagg ctggcgttgg
     5041 tataggcttc ggtgtattcg cggttgaatt tttcattgtt cagcaggtac agcaatacgc
     5101 ctgacaggaa agcaatgtca gtaccggaac gaataggggc atagtagtca gctaccgccg
     5161 ccgtacgcgt aaagcgagga tcgatcacaa tcagcttcgc gccattgtga attttggctt
     5221 ccatcgccca gcggaacccg accgggtgag cttcagcggc gttaccgccc atcaccacga
     5281 cgaggttggc gttcttgatg tcgacccagt ggttggtcat cgcaccgcga ccaaatgttg
     5341 gagcaagact tgctaccgtt ggtccgtgtc agacacgcgc ctggttgtcg accgcgagca
     5401 tacccagcgc gcgggagaat ttttgcgtta aatagccggt ttcgttactc gacgcggaag
     5461 cacacagcat cccggtggag agccagcggt taacggtcac gccttcggcg ttttgcgcaa
     5521 tgtagttagc atcgcggtct tctttcatca gtttggcgat gcgatcaaac gcctcttccc
     5581 aactgatttg ctgccattta tcagaacctg gcgcacggta ttccggaaac ttcagacggc
     5641 tttcggagtg gatgaaatcc accaggccag cgcctttcgg acaaagtgca ccgcggttga
     5701 ccgggtgatc cgggtcacct tcgatatgga agatagatgc tttggcgttt tttgctccgt
     5761 caccgaggct gtacatcaac agcccacagc ctacggaaca ataggtgcag gtattacggg
     5821 tttcgcgggt gcgcagcagt ttatactgcc gggtttccgc gagtgctacg ctgggtgcaa
     5881 aacccagtgc cgctgccgtg gtgcctgcca taccgccagc gcagatctta aagaactgcc
     5941 ttctgctgac ctgcatggat tgctccttgt ttcgacattg tcacgtccca tttacattcg
     6001 cttgctgcgt gtgcagggag tgggagttat ttttctttgc ggaaggggcc gcaaaggtcc
     6061 agaattggct caatttccct ccatccagga agggtttgta acagaatacc ataatgttgg
     6121 tgtgtgtgtt cttatctggt taagagaaag tgaaaaaaac acagcaaaaa gaaatcgaaa
     6181 atgtgacaaa tatcacaggt gttcgtcaaa ttgagttatg gcggcatgat gatttacaac
     6241 acccacggtt agatgaggtg gcggaagaag ttcccgttgc gctggtctac aacggtattt
     6301 cgcatgtggt gatgatggcg tcgcccaaag accttgagta ctttgcgctc ggtttttcgc
     6361 tttccgaggg gataatcgaa agtccgcgcg atatcttcgg catggatgtc gttccttcct
     6421 gtaatggtct tgaagttcaa attgagcttt ccagccgccg ctttatgggg ttgaaggagc
     6481 gtcgccgggc actggcggga cgtacgggat gtggcgtgtg cggcgtggag caacttaatg
     6541 acatcggaaa accggtgcag ccgctaccgt tcacccagac gtttgatctc aacaaactgg
     6601 atgatgcatt acgtcatctc aatgattttc agccagtggg gcaactgacc ggttgtactc
     6661 acgccgccgc ctggatgttg ccatctggcg aactggtcgg cgggcatgaa gacgtgggtc
     6721 gccatgtggc gctggacaaa ctgttaggcc gccggtcaca agaaggggaa agctggcagc
     6781 aaggtgcggt actggtttcc agccgtgcca gttatgaaat ggtgcaaaag tcggcgatgt
     6841 gcggcgtaga gattttgttt gcggtgtctg ccgcgaccac acttgctgta gaagtggctg
     6901 aacgctgtaa tttgacgctg gtgggctttt gtaaaccggg tcgggcaacg gtttataccc
     6961 atccgcagcg tttgagcaat taattaaaat aagtcctgcg atatatatta aattgcagga
     7021 cttattcatt tcgtgaattt tattatttta tttataaaca aaacgcgtgt agtgtcgtta
     7081 acgcctgcgt tgcaacctat tgatggcgtg gcggtgagtt atattgatgc tgccgtagcg
     7141 ttgggtaata ccattaacga aatggataaa tattacatcc aggaaaatta taaagacgat
     7201 gcgtttgcga aagggaaaac gctgcaccag acattcttaa aaaatctgga agcctttgaa
     7261 cctgtagcag aatcttatca tgcggcgatt caggaaatta atgacaagcg ccagcttacg
     7321 gaattgaaaa atattgaaga aagagaaggg aaaacattcc actactactc tctggcagtc
     7381 atgatttcag cgaaacaaat taataacctg atatcgcaag ataagtttga tgcagaagcg
     7441 gcaatgaaga aagtgtctga actggaaacg ctggtggcgc aggccaaaga agcggataaa
     7501 agcggcatga atttctcgtt tattaattcg gcaggccagt atcaactcga ggctaaaaaa
     7561 tacgttcgcc gcatcagaga taaagtcccg tactctgact gggataaaga gcaacttcag
     7621 gacgcaaact caagctggat ggccgaagac tcatttcccg agagcattat gcgagtacaa
     7681 cgaaatggtc gatgaaatat ttcagtcaat tgtaatcgcg gagcgggttt gtgatgagcg
     7741 cggatatgtc acgaagtcgt gacaatataa agcagag
//
LOCUS       DAZUCC010000215         7753 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZUCC010000215 DAZUCC010000000
VERSION     DAZUCC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.216"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..1140
                     /locus_tag="ACHY09_004246"
     CDS             82..1140
                     /locus_tag="ACHY09_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFS3887133.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKLFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNNQWFEEHRTAIESTGATLT
                     TGATGIDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTRLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSALPMAVFNHPEHRLVSPQLYIAAASASESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNQGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDAVIQSARNTFSPVAV
                     RSITDVIVYPESLSNLLN"
     gene            1150..2292
                     /locus_tag="ACHY09_004247"
     CDS             1150..2292
                     /locus_tag="ACHY09_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFS3887134.1"
                     /translation="MSDTLEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPILFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSENALSRRSTLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGKWDIQGKQSWGRLGMLAPAADLGHEVSFRKGER
                     VLVLGTGEFVWEPFLLAERLEAAGAQAFYGATTRSPIAVGYAIKSAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTRLLKALAEVAPVVEIVTYE"
     gene            2285..3058
                     /locus_tag="ACHY09_004248"
     CDS             2285..3058
                     /locus_tag="ACHY09_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887135.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLTPYQEKLASMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFSTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3060..4139
                     /locus_tag="ACHY09_004249"
     CDS             3060..4139
                     /locus_tag="ACHY09_004249"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFS3887136.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVDQKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGTALDIIEERHGTSGIVFVDGWTGKG
                     AITGELVRALRDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLLSR
                     SVWSSEGLHGCMVCEHLSEFECSRMLVDTVAHFRKKLTPSSLAPLSWNTESTRVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIIVTEMGGTLGQYRAVTIIKKVF"
     gene            4139..5095
                     /locus_tag="ACHY09_004250"
     CDS             4139..5095
                     /locus_tag="ACHY09_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFS3887137.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHEKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMRSLGKNNWPLLFIRPRHAEMGKWLKEHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMGGTHLSMMPTLETENVFDVIQMRELATRLLEHPCHKRI
                     IALRIGGNDLMNVVSLRRPRALTLYDSPMGYVIKMLVSVFSPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPDQIEVIQNALMVTQGEYSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLHNEQSADGIRLLTITQHH"
     gene            5106..6201
                     /locus_tag="ACHY09_004251"
                     /pseudo
     CDS             5106..6201
                     /locus_tag="ACHY09_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6219..6686
                     /gene="terW"
                     /locus_tag="ACHY09_004252"
     CDS             6219..6686
                     /gene="terW"
                     /locus_tag="ACHY09_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFS3887138.1"
                     /translation="MQLNTRQARIFKLANLLGSGKPVSATDIITYLDCSEPTLTRALK
                     ELRKSYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNADLKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSGSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7055..7708
                     /locus_tag="ACHY09_004253"
                     /pseudo
     CDS             7055..7708
                     /locus_tag="ACHY09_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         1961 a         1931 c         1978 g         1883 t
ORIGIN      
        1 attatgattg acatgtagca tataccccca taaactcact tctcgacaat aaattttctt
       61 gccgttccgg ataataaaaa aatgaacaaa aaaatttggt ttatggaagg tttgtcctct
      121 cagagagata ttattcaggg ggttaaatta tttgcacaaa aaaataattt tgccattacc
      181 gtttttgcct cccaccgtaa cgaaagaaat gaaatccttt ccgttgccga ttattctttg
      241 actgaacctg aagatcctca aaaacgtctt cagtttattc aggaaaccat tcagacctac
      301 ggcatccacc atattcatac tggtcgtaac aaccagtggt ttgaagaaca ccgaaccgcc
      361 attgaatcga ccggtgccac ccttactacc ggtgcaacgg gcatcgactg gttaactctg
      421 gctgacgaaa aagttacttt tgctcagttt atggagcaaa aggggctccc ggtcgtacca
      481 tcctggcggg tgaatacgct ggcagaatta aagacacgcc tcgcggctcc gccgttcact
      541 gacagtccgg tatgcgtgaa gccggtgacg ggtatctacg gcatgggatt ctggcgcttt
      601 gatgacagtg ctttgcctat ggccgtcttt aatcaccccg aacatcgtct ggtcagcccg
      661 caactgtata ttgctgcagc gtcggcttct gagtcgttta aaccccttgt tttgatgccg
      721 tacctgcctg gcccggaatt ttccgtcgat atcctcgcgg ataagggcga aatactcgca
      781 gccgtgggac gccgtaagga aggggctatc cagtatctgg taaaccaagg aagcgcctgg
      841 gaactggcgt gtgactgcgc ccgggttatg aaggccgacg ggctggtgaa tgttcagacg
      901 cgaaacgatg tgaatggcaa cccggtgctg cttgaaacca acatgcgtcc gtcaggcggg
      961 gtgggttata cccttcacag cggggttaac cttcccgggt tgtttgctgc ttttaagctc
     1021 ggtctgatgt ctgaagatgc ggtgatccag agcgccagaa ataccttttc tccggttgca
     1081 gtgagatcca tcacagatgt catcgtttac ccagaatcac tctctaacct tctgaattaa
     1141 ggcgtgttta tgtctgatac cctcgaagat atgatttatc gccgtaccct ctcatgcggt
     1201 acgattcagg taacccgcga ccagggtgaa gtttcgctcg atgacctgtt tgatatcgct
     1261 gaaagacgta acccgaaacg cgcctttctg tttgtcagca aagtactcgg caggcacatt
     1321 cccgtgccac cctcagtcat gcggcaggcc tacagacagc ttgccagcca gttcccctca
     1381 acgctgacag ggcccatact gtttatcggt atggcagaaa ccgccgttgg gcttggggct
     1441 ggtgtgtttg atgaagtgcg ccaccagcat cgtgaatctg tctatctgac ctctacccgt
     1501 cacccggttg atggcacatt gctttgcgag ttcaaggaag aacacagcca cgccaccgat
     1561 catctgatct atctgccaga cgatgaagag aaaagacgtc gtgttacaaa cgcgcgaact
     1621 ctggttttga ttgatgacga agcaaccacc ggtaatacct ttattaacct gctttctgcc
     1681 ctgcgtaata ccggcaagct tcaacatatt gaacaggtta tcgctgttac gcttacggac
     1741 tggagcgaaa atgccctgtc caggcgcagc actttacccg tcacttcggt ttctcttgta
     1801 agtggtcagt ggggatggac tccattacca gatgcacctg tcccggacat gccgaaggtc
     1861 aacgtaactt cacgtggaaa atgggacatc cagggaaaac agtcctgggg tcgactgggg
     1921 atgctcgcac ctgcggccga tctcggtcat gaggtatcct tccgcaaggg ggagcgtgtt
     1981 ctggttctcg ggacagggga attcgtctgg gagccgttcc tgcttgctga acggctcgaa
     2041 gctgccggag cacaggcatt ttatggtgcg accacccgct cccctatcgc cgttggttat
     2101 gccattaagt ccgcaatttc ctttacggat aactacggcc tgggcatccc caattttgtc
     2161 tataacgtcg cccatcagca atttgaccgc attcttgtgt gtactgagac acctgcagaa
     2221 agtattgaca cgcggcttct taaagcgctg gctgaggttg cacccgtcgt ggagattgtt
     2281 acctatgaat aaacccgttg ttctgagcga ccttgacgac acgctctttc agacccgtcg
     2341 taaaatggta gacgagctgg ccctggagcc attccgtacc ggtgccgttg atcgtactct
     2401 gaatccacga agctttatga cagaagaaca gtccatgtta gtggactggc tcctagagca
     2461 ggctgaactc attcccgtta ccgcacgggg aactgaagaa atcagccgcg taaggatccc
     2521 tttccactcc tgggcaatca ccacgcacgg ggccgtcatt ctcacgcctg aaggcaaacc
     2581 cgacgaggag tggaaggccc atatgctcgg ccagctaact ccctaccagg aaaagctggc
     2641 atcgatgcag cgtctgatca ctgaaatgat ggacgcaaaa gggatcaatg cctgggcaag
     2701 actgaacttc gaatacggtg aaacggcggt ttatatggtg atgaaacacc gcgacagcac
     2761 tcgccttgac gaactcaatg ccattgcaga tgagatagaa acggtgtttt cgaccgaggg
     2821 cttctacatc caccgcaaca gtaacaacgt agcctggctt cccaccccgg ttgagaaagg
     2881 gctggccgtc agctggctgc ttgaaaaact tcgggctgaa cgcggagttt tccccgtaat
     2941 tggtctgggc gacagcctga gcgatcatcg ttttatgaaa ctgtgcagct ggtttggcat
     3001 cccgcgtcag agtcagtttg cagatgccat ttcacagcga atttttggag aaaattaaaa
     3061 tgcagccaca tgaaacattt accggctcat accagcccgg tgacgtggaa tttctgctaa
     3121 agccggttgt cattgaaatg acgccagttg atcaaaaaga agagctgatt cagtccggga
     3181 agaaacatta ttcggacatg ctcagccagg agccagcgcc gacacaatgg catcttgatt
     3241 tgtttcaccg ggcgctggat cggggcgcag agagacttgc aaaagaagtc acacagctcg
     3301 ctattgctct ggccgaacgc ttcggtgatg agcccatagt actggccagt cttgtcaggg
     3361 ctggcgtacc gctcggcgtt atgctacacc aggctctgcg tgacatggga aaaacctcat
     3421 ggcattacgg catcagcatc atccgggatc gtggaattga cggtacagca cttgatatca
     3481 ttgaagagcg gcatggtacc agcggcattg tctttgttga cggatggaca ggtaaaggcg
     3541 caattaccgg agagcttgta cgcgcactga gagatcgccc gggctatcct gagcagccgc
     3601 gactggttgt cctggccgat ccctgtggct gttcctggct tgcagcaagt gatgatgact
     3661 ggctcattcc ttttggcatc atgggggctc cggtgtcagg cctgctctcc cggtcagtat
     3721 ggtcctctga aggattacat gggtgcatgg tttgcgagca tctcagtgaa ttcgaatgca
     3781 gccggatgct tgtcgatacc gtcgctcatt tccgtaagaa gttaacaccg tcatccctcg
     3841 ctcctctgag ctggaatacg gagtcaaccc gggtcttatg gcagacaagt cgcgacgtta
     3901 tcgcgttcct ggccgatgaa tttaaagtgg acagcgtcaa ccgtattaaa cccggtattg
     3961 ctgaagcaac ccgggctgta ttacgtcggg taccggacca tgtatttgtg cgttcgattg
     4021 acgacccgga cgttgcctta cttgtggggc ttgctcgtga aaagggaata attgttacag
     4081 aaatgggggg aaccctcggc cagtatcggg ctgtaaccat tatcaaaaag gtattctaat
     4141 gaaaaatcgt ctttctccct ggaatcttgg tgccactcta tacatgcctg caacacggga
     4201 agatattgct gatgccgttc tgcacgagaa aatcccgggt ttgcgctctc tggtgatttg
     4261 cctggaggat gctgtcagtg aagcagacat ccccgtcgcc ctcaaaaatc tagagcacct
     4321 gctgcacgaa ctcagtaaca gcatgcgtag cctgggtaaa aataactggc ccctgttgtt
     4381 cattcgcccc cggcatgccg aaatgggcaa atggttaaaa gaacattatg atctttccgc
     4441 tgtcgacggg tttgtgttac cgaaatttac cctaagttcg ttagctgaat ggtgggatat
     4501 catgggcgga actcacctga gcatgatgcc aacgctggaa acagaaaacg tctttgatgt
     4561 gattcagatg cgcgagctgg ccacccggct gttggaacat ccctgccaca agcgcattat
     4621 tgcgcttcga atcggcggca acgatcttat gaacgtcgtg tcgcttcgcc gtccccgagc
     4681 cctgacgctg tatgacagtc cgatgggcta cgtcatcaaa atgctcgttt cagtcttcag
     4741 cccgcgtgat tttgccctga ccgcgcccgt atgtgagcat attgacgatc atgccgttat
     4801 ggccagagag ctggctcttg atatggcaca tggacttgtt ggaaaaacgg ccattcatcc
     4861 agatcagata gaggtcattc aaaacgcgct gatggtcact cagggtgagt actctgacgc
     4921 tctgcggatc ctgaactcta cccaggccgt gtttaagtcg cagggggcga tgtgtgaacc
     4981 tgccacacat cgccgctggg cggctggcat tctggacaga gcccggtttt acgggttaca
     5041 taacgagcaa agcgctgatg gaatcagatt acttactata acccagcatc attaaggaaa
     5101 tcataatggg gttcaggttc agaggtactt ccgtttcttt ctgtagccgg ggggcctatg
     5161 ccaatctggg gttacccggt accgagctga gttaccgtac ccggtttgag ctggccgcgc
     5221 cttccggagg tggaaacagg acagcaaccg tcccggatct cagacagtcg cttgagcagg
     5281 aagcagttga actcatgtca gcggtgaccg caattcgtaa cattcacgag ttgacgctgg
     5341 atccaaaaac aggcatcagt tgggcagagc tggaagcagt atacctgcat agcagaacat
     5401 cgctttttca ggttccggca ccagtgcgtc ctgaaaaacc agaataccat gcattgtcgg
     5461 aaaatcctgc agggaacaaa ggcattgttt ttctgggtaa atggtttgaa tcggaatcag
     5521 ctaaagctga gcgtcaagca gggaatcttc gccgatggcg gcaagaactg attgatgtga
     5581 agcgtgagaa tccccttcga cagcaccggt accagcaacg gcagacgacc agggccgaac
     5641 agtattcaaa ctggaagtgt gatgctgaag aacatgaaaa aaggctcgcc acgactcagg
     5701 cagatgcccg gcagttgttc cgggcagacg cagcgttttt cgaatcatac cttgcgggtg
     5761 tgctttcaga aactgaatgg ccgcgtgaaa cgcttgttgc atttgaagta aagccggaga
     5821 tatcaacagt cctgccggat gttggtttag ctgagattga agatttccct gataagattt
     5881 acggcgtcaa tgcccggggc atggagctga cgcaaaaagc catacgcgaa aactatgccc
     5941 gccacagcca tggctgcttg ttccgactgg tcggtatcgt gttacatacg ctacctttcg
     6001 acaaggtgat cgcatcagga tttacacaac gagtaagcgg aacgactatc tggaggatgt
     6061 ttatatcctg tcctgcaaat gcactcgcag ccagaaggca tagaacatat tgatccggtt
     6121 gaagcgttag gctatcaccc tgttattcga aagatgagaa acacattcat ttttcagcct
     6181 attgaaccac taacccttta agcaaactaa atattacgat gcaattaaat accagacagg
     6241 ccagaatttt taagcttgcc aacttactgg gctccggcaa gcctgtttcc gctaccgaca
     6301 ttataaccta tctggactgc tcagagccga cattaacacg ggccctcaaa gagctacgaa
     6361 agtcatattc tgcagaaatc aaatacagta aggcaggtca ttcctatcat ctcgttaatc
     6421 cgggtcaact ggataaaaag acacttcgcc ggatgaatga ggcgcttgca cagaatgctg
     6481 atctgaaaac cggtgagtct acaggaaagg tcgtactcga taaggataaa aaaacagccg
     6541 tatcattatc gctacgcatg cgaattttaa gaaaaattga ccgtctggct gcactgagtg
     6601 gatcgacccg aagtgaagct gttgaaaaac tggcactgca ttccgttgac gaactgataa
     6661 aagaatacag cgctaaaaaa tcctgatgac aaatctcagc tcggggtgaa taccccggtt
     6721 ttttcatatt tcaactctgt cttggcgtga cgtatttttg atctattctc ctcccgcaat
     6781 gtcagcagct ccttacagcc tacaaaacct agcgtcttga catcctcttg tgctacgcat
     6841 caatctttgg gcgatttaca ctccactcat gcaaatctgt gatgtatgat gattttcagt
     6901 aagtgtaaac tgactgccgt atgtagccat taagccagta ttggtaacgt agatgcccac
     6961 cttttcactt atttggtgga caccactttg tctaactcgt cagattctga ccaaacgttt
     7021 caggctgtac gcttactctg aaggggggag aatagtagcc cgtgatagca ttgagctaac
     7081 tgacagccag atcgcagcac taaagcgtaa agccagtgat gacgaggcct gtggcgaaag
     7141 cgaaacggct catccgggat atctgggttc gcaggacacg ttctatgtag gcaacctgaa
     7201 aggtgtcggg cgtatttatc agcagacgtt cgttgatacg tactcgaaag tggctcactg
     7261 taagctttac gtcaccaaaa caccgattac cgcggctgac ttactgaatg atcgtgtact
     7321 gccgttttat acgtctcagg gcctgccgat gctgagaata ctgaccgaca gaggcacgga
     7381 gtactgtggc aaagtagagc agcatgatta tcagctttat ctggcaataa acgatataga
     7441 acacacgaaa acaaaggcga tgtccccgca gaccaacggc atctgcgagc ggttccataa
     7501 aacaatcctg aacgaatttt atcaggtaac gttccgtaaa aaactgtacg gcgatcctga
     7561 tacattacag tcggatcttg atgaatggct ggctcactat aataatgagc gaagccatca
     7621 gagaaaaatg tgcaatgggc ggacgccaat ggaaacgtta cttgatggaa agcgcatctg
     7681 ggccgagaaa aatttaagcc agatgtaatc tgacagatac ctgtataaat aaccggtaac
     7741 tgtcagatca ggt
//
LOCUS       DAZUCC010000216         7717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZUCC010000216 DAZUCC010000000
VERSION     DAZUCC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.217"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..292
                     /locus_tag="ACHY09_004254"
     CDS             <1..292
                     /locus_tag="ACHY09_004254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887139.1"
                     /translation="DKVLITACNHWVLNSRDIIDIFNLSIKHDDYGEINRMYYWLPCE
                     IRGRLMYDGIEWSFVINAAATAMWMSNDKEIYWGCNKKACDNFFLLPYDGMR"
     gene            398..1612
                     /locus_tag="ACHY09_004255"
     CDS             398..1612
                     /locus_tag="ACHY09_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887140.1"
                     /translation="MPIRLNTIPDAEPYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVGSVRWLVFVTQHIRADAWNQRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARHQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGMWTVELNREQRASLLSS
                     NQPFTQEALMYELDAFLGCSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQNGHYSWAT
                     MVSPFVSPQEAS"
     gene            1669..4995
                     /locus_tag="ACHY09_004256"
     CDS             1669..4995
                     /locus_tag="ACHY09_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFS3887141.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQSFELHRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSTEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQT
                     GRPLQSVGCLLSERCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GIAHWQSILKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSPVWAGVTGDNAR
                     GRTVGFPWLRITLMSAVCVLAIWGAGMTTSFFANRALVQETSIQTARALDIRLPLSEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADDLRY
                     AGVPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDMLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGSLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWDQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            4988..6598
                     /gene="tssA"
                     /locus_tag="ACHY09_004257"
     CDS             4988..6598
                     /gene="tssA"
                     /locus_tag="ACHY09_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFS3887142.1"
                     /translation="MSELLQKLTRSCFADRDALDSARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGAQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFTAWPEDQHSPSLMPLINALESRLAQSGRMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADELTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDIAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            6604..>7717
                     /locus_tag="ACHY09_004258"
     CDS             6604..>7717
                     /locus_tag="ACHY09_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFS3887143.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEALSLEVLDGNEVIYSERQTGKYCDKGEHAWQWDGYSNQGILDTTKIKS
                     KSLLVRLIALCGDMMIKVDYPLKNAAKEEEWVDVKVDRNNKVVDITWRLSVKDSGTKG
                     SNPELSPIPYSDLVSLTKNGVEFYWSRNGSRGGGIGGNIVTAIGVFKVNVKAEINITP
                     SMRTFDLISSLDPDMQSSVSLLGFEKIYHNYGGYYNN"
BASE COUNT         1798 a         2034 c         2287 g         1598 t
ORIGIN      
        1 agataaggtg cttattactg cgtgtaatca ttgggtgctt aattctagag atattataga
       61 catattcaat ctaagtataa agcatgatga ttatggcgaa ataaacagaa tgtattattg
      121 gttaccttgt gaaattagag ggagactaat gtatgacgga atagaatgga gttttgttat
      181 caatgctgct gcaacagcaa tgtggatgag caatgataaa gagatttact ggggatgcaa
      241 taaaaaagca tgcgataatt tctttttatt accatatgat gggatgaggt aaatcacagt
      301 taataaagca aaaacattat ggacaagtga attaatcaga tagttcaagc accttgagat
      361 tcattgtatt catatttcag aataacggat tattacaatg cccatccgac ttaataccat
      421 tcccgacgca gaaccctatc ccgcaccgcc agcccgcatt aaatggctgt cagccttatt
      481 actgatgctg tctgttggtg tggcaataac ctcattattc gccagcgatg aactggcaaa
      541 aaacggccca tatttctggg ggctggcctg cggcgtgcct gcttttatct ggtcactggt
      601 cggttcggtg cgctggctgg tatttgtcac tcaacatatt cgtgccgatg cctggaatca
      661 acgtcgcgaa gaagtcattt tgcaggaaac acggcgggga cgacgggcgt tgcagatact
      721 cagtttttcc gtacaaaccg cgctgaatgg tgattccgtc gcagaaacta caacagcatt
      781 tctggcgcgt catcaggtgt tgaatactta cgctgatttg cgcggagaag ataccgtgcg
      841 tcgcagtgtc attcccgtat taaccaataa accggttacc gggcgtttat ccgaaattat
      901 ttcccgcctt tttattgata tcagaccgca attgtttctg ctgccccctg attttcatat
      961 caacgtacta ctggaaattg atgcgccgct ttcaggggcc tccgtgagaa ctatctggca
     1021 ggacgaatgg caaaaagccg gactccccga agcccgtctg ttttctgccc cggagccggg
     1081 gctggccgcc gtggacgact ggctggacaa ctttattcag gaaaaggccg tactgctggt
     1141 gatttccgtg cgtctggaac cgaagaaccc ggaacggacg gcagaatccg ccaccgcgct
     1201 gttgctggca aaccgactga cgcaaaccgc gctgacgccg ctggccctgc tgcatcgtcc
     1261 tgaacgtatc acagatacgg agatgatggc gtcaggcatt gcacaggcgc tggactggat
     1321 gccggtgcag ccagatgcca tatccggcat gtggacagtc gaactgaaca gggaacagcg
     1381 cgcctcgctg ctgtcatcta accagccgtt tactcaggaa gccctgatgt acgagctgga
     1441 tgcctttctg gggtgctccg gaccggcagc gccgtggctg tccgtcgctg cggcaacgct
     1501 ggcggcaata caatcacaac acccccaact gacgctcagc ggcgttcaga acgggcatta
     1561 ttcatgggcg accatggtgt cgccattcgt ttcccctcag gaggcatcat agtggttccg
     1621 ggattaaacc gtctccggcc ccgttcggtt tcagggcctg ccatcgtcat gaccgtggcc
     1681 gttgtggttg cgctttgcac gtttattcac cgtaaaccgg cattgctggg cgttgaaccg
     1741 ggtagttcac agcaaatgta ctggatggct ggcgtgattg ccgcaggtgt ggtctttctg
     1801 attatcatgg gggtttatca tctgctgaac cgccacgctg ccgtacagag ctttgaactc
     1861 cacagacagc ttgccgccgg agatgactat cgggcggtga cgacaaagaa agccgacagc
     1921 cgtcatccgg caatggtgga actcgccgcc ttcctgcgcg accgctacgg cccattctgg
     1981 cggcgtaaaa tccgcctgct gctggtgacc ggcgagcctg aacaggcaga ggccatcgcg
     2041 ccggggctga ccgggcaaca ctggctggag ggcgaccaca cggtgctgat atatggcggc
     2101 aggccatcta cagagcctga tgtcgcgcta ctgaccgcct taaaaaaact gcgccgcagc
     2161 cgtccgctgg acggcatcat ctggccgctg acagaagaac agagccgcca gacggcgcaa
     2221 ctcgacaaag gctggcgcga actgataaac ggcggtaagc gactcggttt tcaggctcca
     2281 ctctatttgt ggcaggtctg tgacaacggt gattatcaga ccggacgccc cctgcaaagc
     2341 gtcggctgcc tgctgtcgga acgctgtacc ccggaacaac tggctgcaat gctggaagca
     2401 caaaccctgc cgctgacgga acagggcatg tcgcagctgc ttaccgataa ccgccatgat
     2461 ttcctgctgc gtctggccca tacccttgca gagcggggta tcgctcactg gcaaagtatc
     2521 ctgaaaccgc tgctggcagg cggcgcattt tcctccctgc gcctgcgtgg cctgatgttc
     2581 agcccgccgc ttaccgccgt accggaagcc gcacctcatg cgtggctgcc gtcaccggtc
     2641 tgggcgggcg tgaccggcga taacgcgcgg ggtcgcacgg tgggctttcc gtggctgcgt
     2701 atcacgctga tgtctgctgt ctgcgtactg gcgatatggg gggccggaat gacgacctcg
     2761 ttcttcgcca accgcgcact tgttcaggaa accagcatcc agacggcgcg tgcactcgat
     2821 atccgtctgc cgctgtctga acaactggtg gcgctgcata ccctgcaggg tgaactggag
     2881 cgcctgcagt accgtatccg ggagggtgcg ccgtggtatc agcgttttgg ccttgaacgt
     2941 aaccagcaac tgctcgccgc cgcctttccc ggctatgcgc aggcggcaaa ccggctggtg
     3001 cgcgacgtgg ccgttgacca tctgcaacag caactgaatg cctttgtcgc gctgccgccc
     3061 aatagtccgc agcgtaccgc caccggcgaa cagcgctata agcagcttaa ggcattgctg
     3121 atgacttccc gcccggaaaa ggccgacgct gcctttttca gtaccacgct gatggcagat
     3181 gacctgcgct atgcaggtgt accggaaggt gtgcgtcaga gcgtgttgcc gtcactgctg
     3241 accttctgga tggcgaatct gccggaacac ccacagtgga aaacttcgcc gccaccggaa
     3301 ctgaccggcg cagtgcgcaa aatcctgctg cgccagattg gtgtgcgcaa tgccgaaaac
     3361 actctctacc agaacgtact gcaacaggtg tcccgcaact acgccgatat gacgctggcg
     3421 gacatgaccg gggatacgct caccgaatct cttttcagta cggagcagac ggtgccgggg
     3481 atgtttaccc gtcaggcgtg ggaaggacag gtcagggaag ccatcgagca ggtggtgacg
     3541 gcgcggcgcg aggaaatcga ctgggtactc agcgaccgcc agcaggatac ctctgcggat
     3601 atctcgccgg atatgctgcg taaccgtctc acttcacgct actttaccga ctttgccgga
     3661 agctggctgg cgtttctcaa cagcattcgc tggaaaaagg aagactcgct ctccggcatt
     3721 ctcgaccagt tgacgctgat ggccgatgcc cgccagtcac cgctgattgc gctgacagac
     3781 accctcgcgt ggcaggcggc ggctggcagg gaaaaccgtg gcctgtcaga ctcgctggcg
     3841 aaatcggcac aggaactgtt taacggcaag gagaaaacgc cgcagcaatc ccgtgaaggc
     3901 gacgtgcctg tcgggccgct ggataaaacc ttcgcgccgc tgctgcggtt gcttggcgat
     3961 aaggcaggag gcggcgacag ccagctgagc ctgcagacct acctcacccg agtcacccgc
     4021 gtgcgcctca aactgcaaca ggtgaccaac gcccctgacc cgcaggagat gacccagcaa
     4081 ctggcgcaga cggtcttaca aggcaaaacc gttgacctta ccgacacccg cgactatggc
     4141 agcctgatag ccgccagtct gggtgaagag tggagtggct tcggtcaggc gctgttcgtc
     4201 cgcccggtgg agcagtcatg gcggcaggtg ctgacgcctg cggcagacag cctgaaccgc
     4261 cagtggcagc gggcgattgt cagccactgg gatcaggact tcgctggtcg ctacccgttc
     4321 aaagcctcac aaaacgatgc ctccctcccc ctgctggcgc agtacctgcg cgatgacggg
     4381 cgcatcaacc agtttatcgc cgccaatctt tccggcgtac tgaaacgtga aggccgctac
     4441 tgggtggctg acgccatgaa cacgcagggg ctgacagtca atccggactt tattcgcgcc
     4501 ctgaaccgcc tgcgcgatgt ggccgatacc gcctttgcca gcggcgatgc agggatacat
     4561 tttgaactgc gggcaaaacc ggcgcgcgac gtgatgaaga cgcatctggt gattgacggg
     4621 caggagctgg aatatttcaa ccagaaggaa cgctggcagc gttttaactg gccggatgaa
     4681 cagtggcaac ccggcgcatc gctgagctgg accagtacgc aggcgatgga acgcatactg
     4741 gcggattatc ggggaagctg gagccttatt cgcctgctgg aacaggcgca ggtgacaccg
     4801 gtggacagca gtacctttaa agtggtgtgg aaagcgcagg acggcctgcc gctgaattac
     4861 ctgctgcggg ttgaacaggg taaagggccg ctggcgctgc tggaactgaa aaacttccgc
     4921 ctgccgggac aggtgttcct gaccggcagg agtatgaagg atgcggaaga atacggggaa
     4981 gacgccgatg agtgaactgt tgcaaaagct gacccgtagc tgttttgccg accgggacgc
     5041 actggactcg gcgcgaacac aggcggcgct gtggcagaca tggctgctgc cggtgacggc
     5101 agatacgccg gtcggggaag accccggcta ccacgacgat ttcctgcgta tccgcgacga
     5161 gatgaacaaa ctttccggcg cggatacgga ccttatctgc cagcttgccg aatccctgct
     5221 gctgacgcag gcaaaagacg tgcgcatcgc cacttactat atctgggcgc gactgcaccg
     5281 cgatggcgaa cgcggactgg ctgaggggct ggcgctgtta gccggactgg tggaacgctt
     5341 cggtgcgcaa ctgctgccct cgcgcccggc aagccgaaaa atggcgctgg agtggctggc
     5401 aggggaaaag atgctcgaca gccttgcccg ctacccggaa gtggcaaaag aggattttgc
     5461 gaacattgtt gccgctctta atcaactgac ggtttccttc actgcatggc ccgaagacca
     5521 gcattccccc tccctgatgc cgctgattaa cgcgctggaa tcacgtctgg cgcagtcggg
     5581 taggatgaac gcggtggtgc cgcagaacag cagcggcgtt ccggcaccgt cttccccggt
     5641 ggatgcgcca caggtacaga ccatcacttc cgggcgcgac ctgctcgacc aggcgaaagt
     5701 gctggcccgt tatctgaacg aacagccgca gggctggctt tcagcacacc ggctgatgaa
     5761 aaccctgcgc tgggacacgg tgcatgaact gccgccggat gtggacggaa aaacccggct
     5821 ggccccgccg cgcacagaat cacgcaacca gcttaagcgg ctgtatgctc agcagaactg
     5881 gacggaactg ctggagcagg ccgacctgat gttcagcacc ggcgtcagcc atttctggct
     5941 ggacattcag tggtatctgc atcaggcgct gactaaggcg ggcgcaccgt gggacaggtg
     6001 gacagcggtt atccgtcagg acctggcgtt actactggaa cgcctgccgg ggctggaaaa
     6061 tctggcatgg aacgacggca cgccgttcgc cgatgagttg acgcgcaact ggatagcgca
     6121 gcaggtcatg atgcgggaag acggcgcatg gcttgcgggg aaagccgctg taccgacaga
     6181 cgacgccaca aacgacgtac tggcgctgga gccggaagcg ctggagatgg cagacagtca
     6241 gggcgtggaa gcggcgctgg gctggataca gacccgtccg ggcatcacca cggcacgcca
     6301 gcgcctgtta ttgcgactgc tgatggcgcg ggtggcggaa cagtacggta aaaacgagat
     6361 ggcgctcctg ctgctggaag aactggatat cgccgcacag gggctgacgc tgacgcagtg
     6421 ggagccggac ctgctgtttg aggtaaaagc ccgacagctg aaactgctgc gcctgcgggc
     6481 gcaccgctat gcggataaag ccctgctgaa ccgaaaaatg gaaatcctgc tgggaacgct
     6541 ggtgacaata gatccggtac gggcagcggt attgtgtgat acgcaacata aggactgatg
     6601 gcaatgggaa caggctactt tctggtcagg ggggacaaaa ccacctgcgg cggcaaaatt
     6661 attgaaggcg cggacgacca tacgatcatg ggtatcccgc aggcgcggga tatggaccgg
     6721 gtcacctgcg gcagatatcc gggaatgttt attattgtgg gcggcgttcc tgaaacagat
     6781 attcacggca ggctgatggc ggggacgctg gacagtcaga gcagttgccc gtgtaaggca
     6841 cggtttattg cgtcaatgat ggatgatacg tatgagacgg atgacgggga aagtgaagcg
     6901 gaacaacatg cgcagtcggc gaaaaaggat ctgacttctg gttctgattc atcctctgat
     6961 gatgtaaaac tggattacag aattaaatta tcaggtaata agatactgac gccactgaat
     7021 atacctgatt ataaagaaat gattagcggt ggtagtacga agaatacaga aaaaatagat
     7081 tttaccatca caaataaagg cgatgaggct gaagcgcttt ctctggaagt gcttgatggt
     7141 aatgaagtga tttatagtga acggcagacc ggtaaatatt gtgataaggg agagcatgcc
     7201 tggcaatggg atggctacag taatcaaggc atactggata caactaagat aaaaagcaaa
     7261 tcactactcg taagactgat tgctttatgt ggtgatatga tgataaaagt tgattatcca
     7321 ttgaaaaatg ctgcgaaaga agaagagtgg gttgatgtaa aagttgacag aaataataaa
     7381 gtggtcgata taacatggag attatctgtt aaggatagcg gtacaaaagg cagtaaccct
     7441 gagttatcgc caatacctta tagcgattta gttagtctta ctaaaaatgg cgtggagttt
     7501 tactggtcac gaaatggaag tcgcggcggg ggaattggtg gtaatatcgt tacagcgatt
     7561 ggggttttta aagtaaatgt aaaggctgag ataaatatta ctccatctat gcgaactttt
     7621 gatttgatat catcgttaga tccagatatg cagtcgtcgg taagtctttt ggggtttgaa
     7681 aaaatatacc ataactatgg tggctattat aataaca
//
LOCUS       DAZUCC010000217         7646 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZUCC010000217 DAZUCC010000000
VERSION     DAZUCC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.218"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            177..2318
                     /locus_tag="ACHY09_004259"
     CDS             177..2318
                     /locus_tag="ACHY09_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFS3887144.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2426..3757)
                     /locus_tag="ACHY09_004260"
     CDS             complement(2426..3757)
                     /locus_tag="ACHY09_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3887145.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPNTDY
                     SVKLLWAWSTYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAFMDYQPGV
                     EQTPQALWGICLLMTIIPAILNLITGLIMRFYLINNEYYEQIKVCLQPKNI"
     gene            4044..4796
                     /locus_tag="ACHY09_004261"
     CDS             4044..4796
                     /locus_tag="ACHY09_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS3887146.1"
                     /translation="MIKIYKASKGQLKHREIFDEIKKKVDNGEWKEGKAIPTERELSE
                     YYLSSRTTIRKAIESLKQRGYLHSVHGQGTFVLPSRSRENHLLHSFTDDIKARGGIPA
                     QNILEIGYVPVSDVIRKNLDLDIHVQTVFCIKRVRYMGSTPLGIQTSWLALNDRQEIT
                     QEDLLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            4810..5886
                     /locus_tag="ACHY09_004262"
     CDS             4810..5886
                     /locus_tag="ACHY09_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFS3887147.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWSGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALEAQEK"
     gene            5886..6884
                     /locus_tag="ACHY09_004263"
     CDS             5886..6884
                     /locus_tag="ACHY09_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS3887148.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEDDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            6889..>7646
                     /locus_tag="ACHY09_004264"
     CDS             6889..>7646
                     /locus_tag="ACHY09_004264"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFS3887149.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTF"
BASE COUNT         2265 a         1700 c         1693 g         1988 t
ORIGIN      
        1 ataaaaacac atgtagcaat tttttgttat ttctcattta ctactacatt actgcgtgat
       61 tgaatgattc tcaattacat atccgatggc gaaataacaa acggatagtg cttagtgagc
      121 aataagaaac aattttcact cgcgacaatc cgcgagtttt tgggataaca gactaaatgg
      181 ctatgttcac accttcattc tcaggactca aaggtcgggc gctcttttca ctgctttttg
      241 cggcaccgat gattcatgca acagactctg taacgaccaa agatggcgaa acaatcactg
      301 ttacagcaga tgcaaatacc gcaactgagg cgaccgatgg ttatcaacct ctgagcactt
      361 ccacggcgac attaaccgat atgccgatgc tggatatccc gcaggtggtc aatacggtta
      421 gcgatcaggt tctggaaaac cagaatgcga caacgctgga tgaggcgctt tataacgtca
      481 gtaacgtggt acagaccaat acattaggcg ggactcagga tgcctttgta cgccgtgggt
      541 ttggcgcaaa ccgggatggc tccatcatga ccaacggtct gcgaaccgta cttcctcgca
      601 gtttcaacgc cgccacagaa cgtgtggaag tgctaaaagg cccggcctcc acgctgtatg
      661 gcattctcga tcctggcgga ctgattaacg tcgtgaccaa gcgcccggaa aaaacattcc
      721 atggttcggt ttcagccacc tcctccagtt ttggtggcgg cactgggcaa cttgatatca
      781 caggtcccat tgaaggcact cagctggcgt atcgccttac cggggaagtg caggatgaag
      841 attactggcg aaacttcggt aaagagcgca gtacatttat tgccccgtca ctcacctggt
      901 ttggtgataa tgcaacagta accatgctct attcccatcg ggactataaa actccgttcg
      961 atcgtggaac gattttcgac cttacgacga aacagcccgt aaacgttgat cgaaaaatac
     1021 gttttgacga accgtttaat attacagatg gtcagtccga tctggcgcaa ctcaacgcag
     1081 aatatcatct caatagccag tggacagcgc gctttgatta cagctacagc caggataaat
     1141 acagcgataa tcaggcgcgt gttaccgcgt atgatgcaac gacaggaaca ctgacacggc
     1201 gtgttgatgc aactcaggga tctacccagc gtatgcatgc tactcgtgcg gatctgcaag
     1261 ggaatgttga tattgccgga ttctataatg agattctggg tggggtgtca tatgaatatt
     1321 atgatcttct gcgtacagat atgattcgct gtaaaaaagc taaagatttc aatatctaca
     1381 accctgttta tggtaatacc agcaaatgta caacggtttc ggcgtcggac agtgatcaga
     1441 cgatcaaaca ggagagctac tcagcgtatg cacaggacgc actctatctg accgataact
     1501 ggattgccgt cgccgggatc cgctatcagt attacacgca gtatgcgggt aaaggccgtc
     1561 cttttaatgt caatactgac agccgcgatg aacaatggac tcccaaactg gggttggtct
     1621 acaaactgac gccatcggta tccttatttg ccaattattc gcaaacattt atgccgcagt
     1681 cgtcaattgc cagctacatt ggcgatcttc caccggaatc atctaatgct tacgaagtcg
     1741 gggcaaaatt cgagctattc gatggtatca ccgcagatat tgcgctgttt gatatccata
     1801 aacgtaatgt gttgtatacc gaaagtgttg gtgatgaaac cattgccaaa acggcaggcc
     1861 gtgttcgttc aagaggggta gaagtcgacc ttgcgggagc attaactgaa aacattaata
     1921 tcattgccag ctacggctat accgatgcta aggttctgga agatcctgat tatgcaggta
     1981 aaccattgcc gaatgttcct cgtcataccg gttcgctatt cctgacctat gacattcata
     2041 acatgcctgg caataacaca ctgacgtttg gcggtggtgg acatggcgta agccgtcgtt
     2101 cggcaaccaa tggggctgac tattatctgc caggctattt tgttgccgat gccttcgccg
     2161 catacaaaat gaaattgcag tatccggtca cactgcaatt aaacgtcaaa aacctgtttg
     2221 ataaaacgta ttacacctct tccatcgcca caaataatct ggggaatcag attggcgatc
     2281 cgcgtgaagt gcaattcacg gtgaaaatgg aattttgatc aaaaaagccc ggtatgttta
     2341 ccgggctgag actgatgaca aactccaaat tgtctgatgc gctacgccca tcagatctac
     2401 catagaaatt caatatatta aattttcata tatttttagg ctgtaaacac accttaatct
     2461 gttcgtaata ttcattatta atcagataaa atctcatgat gagtccggta atcaggttaa
     2521 gtatcgcggg aatgatcgtc attaacaggc agatccccca caacgcctgt ggtgtttgtt
     2581 ctacgccagg ttgatagtcc ataaacgcta aagcgattcc gataaaccct cccgccagtc
     2641 ccataccaaa cttttgaaaa aagagaatcc cgccaaatgc cagtccagaa acacgttgcc
     2701 ccgttttcat ttcaccataa tccactgact cagcaatgga tgcccaataa atgggaagct
     2761 gaatatcagt cagaaaggtc accacaatat aaaatgtaaa agctaacact atagaatttg
     2821 gttgcacgaa atacatcgca ccacccacca cgaacgccag taattgggta taacgaaata
     2881 tttttatttt gtcgtagagt cgagtaagcc aggtgcaagc gatcatagcc agaacagagg
     2941 cgatcacgcc tgaagttaaa aacggtgaaa ttaaagcatc cccaccattc aggtaatatt
     3001 ttgcataata tgcagcgaca gagttacgaa taatcccgcc aaacataatg atcgcaatta
     3061 cagcgcccaa aatgagccat tgatcatttt tgatcagtaa ccgaaactgc gcggccatac
     3121 cgagactggg ttttggatgg tagatgcgct ctctcacgct aaaaaaacaa tacaggcata
     3181 aacacgttgc cagaattccc attgccccca ttgctatttg ataacccagt tgtacgttat
     3241 tcttcccaag atacattgca gataaaggaa caataattgt tacggcgaac atggcaattt
     3301 ttgtcataac aaaacgataa ccattggcgc tcaatcgctc ttgtggatca tccgtgataa
     3361 cgctgattaa tgaaacataa ggaatagcga ttaacgtata ggtcaaggta agtaacacat
     3421 aggtagacca ggcccataaa agcttaactg aataatcggt attaggtgtg gtgaacatca
     3481 accaaatgct tatcccaaac ggtattgaca taaaaagcaa ccacggccga tattttcccc
     3541 aacgtgtatt cgttctgtct gtcattgttc ccacaactgg atcaatcaca gcatcaagga
     3601 tacgtacaga aaataacaga atacctaagt cgacgggttt cagaccaaat acatccgtgt
     3661 aaaaataagt gatgatcatg gccaaagaca tcattactat cgcgatagcc atatcaccag
     3721 caccaaaacc tatcttttca tttattgtaa gtttcataca cagcactcat tgattacgtg
     3781 aacttatatt aaaaacaaat atatctgaac tcattcagca cataacttta cgagtgagca
     3841 taaatgcatt ggttgatacc acaacaaacc attgagtcaa tattgagcaa catcacataa
     3901 aaaccaattt cacttaatct catgatttat atatttaatt gtggtttttt ctgttttatg
     3961 tcacagatag tgaacaagca ccatgcgtaa taaaagttaa agaatgtaga gttggtatca
     4021 accacttgaa atgaggcttt gtcatgataa aaatttataa agccagtaaa ggtcagctaa
     4081 agcatcgtga aatctttgat gaaataaaga aaaaagtaga taacggtgaa tggaaagaag
     4141 gcaaagccat accgacagaa agggaattgt cagaatatta tttaagtagc agaacaacta
     4201 ttcgaaaggc aatagaaagt cttaaacaac gcggctacct gcatagtgtc cacgggcaag
     4261 gaacatttgt attgccttct cgtagtagag aaaatcattt attgcatagt tttaccgacg
     4321 atattaaagc tcgaggtggt ataccagcac agaatattct tgagataggc tatgttcctg
     4381 tcagtgatgt tatcagaaaa aatctcgacc tggatatcca tgtccaaacc gtattctgta
     4441 taaaacgcgt tcgctatatg ggaagtaccc ccctgggtat tcagacatca tggcttgcat
     4501 taaatgacag gcaagaaata acccaagaag atctgctggc gacgggttcg ctctatattt
     4561 tactggaaga aaaactcgct attaaacctt tagaagcaac agaaattatc tctgcccggc
     4621 ttccctcgcc ccttgagcgt caattactgg aactgactgc cgatgatgtc gtattaagtt
     4681 gtacccgcgt cacgttatcc gtggaacgca aaccaatgga gtatgtagag atggtgtatc
     4741 ccgcaagccg gtactcctac aaagcacgaa ttacaaaaga cagttttagc gtgtaacaga
     4801 gagaatattg tgaacaaaat agaacaagct gtaatttata acaaaatatt aggttcttta
     4861 gcctgcgcag gtatgggcga tgcattaggc gcagcgactg aactctacag cattgatgaa
     4921 ataaaagctc aatggggagg ttttttaaat gcctttgttt ctcctcctgc cgatacattc
     4981 gctggctcat taaatggtat tgcgggactt atcaccgacg attccagcca aatgtatgtg
     5041 ttttcagaag ggttaattga ggctggattt gataacttca ccaataatga ctggttagct
     5101 tgtcttctgc gttgggccga tatgcagccc tacgcaaatt ataaaggccc cacaacagaa
     5161 caaatcgtca aggcactaaa agaaggtaga ccaacaaaca ccattgggcg tattggaaca
     5221 tcttctcgtc aggcacccaa tgtcggcacc acgaatggag caggaatgcg tgtagcccct
     5281 gcaggattga tctggtcagg caagaaagaa aaagcgtgtc acctggcgct aataacttgc
     5341 ctaccttcgc atgacaccaa tattgcgatt gccagcgctt gcgccatcgc tgcggcgaca
     5401 tcacaagcta tgttaccaga agcttcgctc acatcattac tggacgccgc aatctgggga
     5461 gcgaattacg gcgaacgcct ggccaaacaa tatgcacgat gtgtcgcagg gccatctatc
     5521 gccatgcgca tccaacttgc agcggacatc gccagacgag ccaatgacct ggagtcttgt
     5581 ctgcgagaaa tggaaggttt ggttggtaac tctgttgccg cccatgaatc cattcctgcg
     5641 gcaatcggtc tattgctgta ctgcaaagga gaaccctggg aaaccattca tgcctgcgcc
     5701 aatattggca atgacaccga tagcatcgct acgatggcag gcgcaattgc aggggcatgg
     5761 cgcggttttg atgcgctccc tgaagataaa tatgcctttt tccgcgccgt aaataataaa
     5821 gatttcgata ttgaagcgat cgcatcgggt ctgacattgc ttgcattaga ggcacaggag
     5881 aaataatgaa catgccagcc cgtactcctg ttatgccatt agcgcaaact cgtgaggaga
     5941 taaatgctac ggcggcatcg ttacgccatt tggcagctat ctggggtgaa tgtttttcta
     6001 cttttcagct cgataaacgt gctgttgaac gcgtctgtat agtgggatcg ggcgactcct
     6061 ggacggtggc actatgtgcc gccgcctggc tggggaaata cacacatctg ttttgttacg
     6121 cattgcaaac gtgggatttt cttcagactg atttaacccg ttatcagaaa gaaacgttaa
     6181 tcattattct cagtgcaagt ggcagaccat caatgaccgt tgacgccctc tgccacgcag
     6241 tatgctcaaa cgcgcaagtg ctgggtgtga cgaattgccc tggaacacca ttttgcgcaa
     6301 taacagaaaa catgctctat acatgggcga ataagcaggg tattccaacg caaagtagta
     6361 gcgtcacact ttatgccctc ctgcgcttag cgcaaaaatt atgtccaggc ttaacaccac
     6421 tgcagattga agatgatttg gaagggaaat ttagccagat taatcagcac tggcagcaaa
     6481 agaagcgcca tttttaccaa caaaaagaga taacgtttct tgggagtggc ttaagttggg
     6541 gactggcaat atcggggagc aatttacttt cttgcggccc acagattcgt gcgacagccc
     6601 ttccactgga agaattttat cattcattac gcttacatca ggctggtgtc ggtcagcatt
     6661 atattttact accagccaca tcctgcgagt ccccatttta ccttgcaacc caaaaagcaa
     6721 ttgttgaaca gggagcgaca gcagaattaa tcagttttat acctgacgcc agtgagtcca
     6781 acaatttatt tctggtcatg cagtggttat atgaaatgtg ctggcattta agttgtgatt
     6841 atgtcgacgc aggcgggcaa cgcgttagcc atagggagaa ataagactat gaagaaattt
     6901 gatgtcgccg ctttaggcag tggaaatata gatatttttt tatctatccc gtcactcccc
     6961 actcgtggcg gtaaagttgt cgggacacgt ctgggtgaac aagctggcgg cacagtcgcc
     7021 aacagtgcct gtgcgatggg acaactgggt ctcaatgtgg tatctgtttc atgtgtgggc
     7081 aatgatcatt cagcctcgat cattcttgat ggatttaaaa aatatcatgt aaattgtgat
     7141 ttcgttcagg taattccgga attgattgcc aataccgcga ttatctttat tgatgaatta
     7201 ggtgaaaaaa cgttggttta ctcgcctggt agtgatcatg agtgggataa agaaaaagcc
     7261 ctgcaggcaa ttgcgcaaag ccgttatttt tatacgatgc cagcagatat cgagaaattt
     7321 cgtatgctgg ccgaatattc ccgtagccag atgacaaaag ttgtcgttga tattgagcct
     7381 catatcattg caacacctga acaccttgcc agaattttac accttgctga tattgcgata
     7441 tttaattatg acggttttat tcgtggctat gcaacagagc cagattttac acttctgcat
     7501 gatatacaag acgagtatca acttgacgct gtcgtggtta cgctggatgt taggggagta
     7561 attgcagtca aaggcaatga gcaagtaaaa ataggtagtt atacaattcc cgttattgat
     7621 accacaggcg ctggagatac ttttaa
//
LOCUS       DAZUCC010000218         7516 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZUCC010000218 DAZUCC010000000
VERSION     DAZUCC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.219"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            171..752
                     /locus_tag="ACHY09_004265"
     CDS             171..752
                     /locus_tag="ACHY09_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFS3887150.1"
                     /translation="MVSLVKNQTVSLSKESPALSQLHFGLGWDPVKKKGFLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLESTCKAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELACYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            752..1909
                     /locus_tag="ACHY09_004266"
     CDS             752..1909
                     /locus_tag="ACHY09_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFS3887151.1"
                     /translation="MNLMPGGNAPVPAQELRVRITSGVQVDASAFRLYAAGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGCVELSGRQEAALILGEFYRRNNGWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVPPLPAETKISLSKVSLTKEMPAISLTKRDNFGEIRINLNWH
                     RGSGKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIYVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            1932..2387
                     /locus_tag="ACHY09_004267"
     CDS             1932..2387
                     /locus_tag="ACHY09_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFS3887152.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFEPAEFGL"
     gene            2406..3446
                     /gene="terC"
                     /locus_tag="ACHY09_004268"
     CDS             2406..3446
                     /gene="terC"
                     /locus_tag="ACHY09_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFS3887153.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPVWPKLRGHAFLLNQKEVDAELAKPENSDVTIDRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLIHLEKAVIVLLFFIAVKLGLNATDHIWHHGYSIAATTSLYVVLGVLVLGILASVI
                     FPGKPESEEKGN"
     gene            3495..4073
                     /gene="terD"
                     /locus_tag="ACHY09_004269"
     CDS             3495..4073
                     /gene="terD"
                     /locus_tag="ACHY09_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFS3887154.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLTANGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDTVPSDI
                     DRIIFVVTIHDAQARRQNFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            4141..4716
                     /locus_tag="ACHY09_004270"
     CDS             4141..4716
                     /locus_tag="ACHY09_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFS3887155.1"
                     /translation="MAVSLVKGGNISLTKEAPTMNVAMVGLGWDARVTDGQAFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQIKIDLAKVSVDV
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGAEIARFDLSEDASTETAMVFGEL
                     YRHGAEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            4830..6124
                     /locus_tag="ACHY09_004271"
                     /pseudo
     CDS             4830..6124
                     /locus_tag="ACHY09_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            6173..7381
                     /locus_tag="ACHY09_004272"
     CDS             6173..7381
                     /locus_tag="ACHY09_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS3887156.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMT
                     HHLGYEKNQSKPGTNSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTLITG
                     MDNQIFSLYAKGMTTREIAAAFKELYDADVSPALISKVTDAVMEQVVEWQNRPSDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELPGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPGAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRN
                     LKAIYQAPTEEAGQQAQEAFAASWDSRYPHISRSWQANWPNLATFFAYATGIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQSASQKWTMPLKDWRMAMSRFII
                     DFGDRLGGHF"
BASE COUNT         1843 a         1802 c         2021 g         1850 t
ORIGIN      
        1 ttaattggta tttagatgaa aggacatcat gaaaaggatt tctctttgtg tttttacaga
       61 aagctaccaa atttcccttg tcagagaact tcatcatgat atgattttct tcgccagtta
      121 ctaaatatac ttttgattgt ctcagttatc agcattttaa ggaataaaac atggtttcat
      181 tagttaagaa tcagacggta tcactcagca aagaatcacc tgcattaagc cagcttcatt
      241 tcggtctcgg ttgggatccg gttaagaaaa aaggttttct tggaggattg tttggaggta
      301 acgactcaat cgatctcgat gctggctgtg tattaatgga cagcaccggt aaaacaattg
      361 acaccatctg gttccgcaaa ctggaatcca cctgcaaagc tgttgtgcat agcggtgata
      421 acctgactgg tgagggtgac ggcgacgatg aagtgattaa ggtcaacctc tcccgtctgc
      481 ctgcaaacgt tgaatacctg gcgtttacgg ttaacagctt ccgtgggcag tcgttcaatg
      541 acgtcgaaaa tgcgttctgt cgtgtggtcg atcaaaccgg caaagagctg gcttgctaca
      601 agctgactga gcagggctca cataccggga tcgttatttc ttcgctgcgc cgtaataacg
      661 gcaactggga ctttaccgcc cttggtcatg cctgtcgcgg tcgcacaatt gacgatatgc
      721 actccgatat cgtttctgcg gttattcgct aatgaacctg atgccggggg gcaatgcccc
      781 cgttccagcc caagagttgc gggtccggat aacctcgggc gtccaggtgg atgcttcagc
      841 gtttcgcctg tatgccgccg ggaaggtgca gggggatgcg gacatggtgt tttatggtca
      901 gccacgtaac gatgatggta ctgtcagcct ggtcagtgaa ggtcagtact caacctttac
      961 tgtcgcgctc aaccggctga aacctgatgt ccagaaaatt gcgttcaccg tgacctgtga
     1021 cggtgggcag acggtgtccg gtcttcgtaa tctttccatc gacgtggagc aaggggctac
     1081 cggcctggtg agcggctgtg tcgaactgag cggccgtcag gaagcagcct taattctggg
     1141 tgaattttac cgtcgtaata atggctggaa attccgcttc gttgcgcagg ggttcaacgg
     1201 tggacttaaa ccgctggcag agcatttcgg cgtaaatatt gccgacgaac cggctcccgc
     1261 agccccgact cctccggttg taccccctct gccagctgag acaaaaatca gcctgagtaa
     1321 agtatctctg acaaaagaga tgcctgcaat cagtctgacc aaacgggata acttcggtga
     1381 aatcaggatc aacctgaact ggcaccgggg cagcggcaaa tccggctttg caggcatgtt
     1441 cggctccaaa ggcatcgacc tggatctggg agcctttgtt gagcttcagg acgggtataa
     1501 gtcggtcatt caggcgctcg gtaatgcatt cggtgattat cgcgatgaac cttatgttca
     1561 gctcaagggc gatgaccgga cgggtgatgt atcagacggc gagtggctgc atatcaatgg
     1621 acgtgaatgg aagcatatcc gcgaagtgct gatttacgcc tttatttatg aaggtgttcc
     1681 cagttgggac aagactgatg gtgtggtcac catttatgtg ccggatcaac cgcccattga
     1741 gacccgcctg actgaaggtg aaaaccgtcg cacattgtgc gccattgcca gactcgtaaa
     1801 cgaaaacggt gcgattaaag tcgagcgtat caaccagtac ttcaaaggcc aggacgaaat
     1861 ggaccgggca tttggctggg gatttcgctg gagcgccggt tctaaataac acagcaataa
     1921 aggaaactgg tatgagcttt ttcgacaaag ttaaaggtgc cattaactct ggccgtgacg
     1981 aactgacccg ccaggttggt cgtttcaaaa acaaaaaatt catgcagggc accgttgctg
     2041 tatgtgcccg tattgccgta tcgagtgacg gcgtgagttc ggaagaaaag cagaaaatga
     2101 tgggctttct gcgctcttca gaagagctga aggtcttcga taccaatgag gtgatcgagt
     2161 tcttcaataa actggtttca agcttcgatt tcgatgttga aatcggcaag ggcgagacca
     2221 tgaaatacat cctggcgctg aaagatcagc ctgaggccgc tcaactggcc ttacgtgttg
     2281 gtattgctgt tgcgaaaagc gacggtaact tcgatcagga cgagaaactg gcctcccgcg
     2341 agatcgctat cgcgttgggc ttcgagccgg ctgaatttgg cctctgacat ccctaagggt
     2401 aaaatatggt ttccacacac atcggcttcc cgactgaaac ggtcattgtt tttattgctc
     2461 tttcagtcgg agccatcttt attgacctgt ttatgcaccg tgatgacaag ccaatctccc
     2521 tgaagagtgc agcgctctgg tccgtattct gggttgttgt tgcgatggca tttgccggtt
     2581 tcctctatat ccaccacggt gctgaagttg ccagtttgtt tgtcacgggg tatgcgctgg
     2641 agaaagtact gtcggtcgac aacctgtttg tcatgatggc catcttttcc tggttcgctg
     2701 ttccggatcg ttatcggcac cgtgtgcttt actgggggat cattggagcg atcgtcttca
     2761 ggggaatctt tgtcgccatc ggtacgagcc tgctgagtct ggggccgtac gttgaagtcg
     2821 tcttcgctat catcgttgcc tggacagcgg tcatgatgct taaaagcggt gatgatgatg
     2881 atgaaattga agattactcc cagcatctgg cttaccgcat ggttaaacgt ttcttccctg
     2941 tctggccgaa gctacgtggg catgccttcc tgcttaacca gaaggaagtg gatgctgaac
     3001 tggcgaaacc agaaaacagt gatgtaacca ttgaccgtgg taaaaaggcg gcgctgtatg
     3061 cgacccctct gttcctgtgt gtggccgtgg ttgaactctc ggatgttatg tttgcgtttg
     3121 actcggtacc ggcaatcatt gctgtcagtc gtgaaccgct tatcgtctat agtgccatga
     3181 tgtttgctat cctggggctg cgtactctgt actttgtcct tgaagcactg aaacagtacc
     3241 tgattcatct ggaaaaggct gttatcgtgt tgttgttctt cattgcggta aaactgggcc
     3301 tgaacgcgac cgatcacatc tggcatcatg gttacagcat cgctgcgaca accagcctgt
     3361 atgttgtact gggggttctg gtgctgggca ttctcgcaag cgttattttc cccggcaaac
     3421 ctgaatctga ggaaaagggg aactgatccc cttaagtgtt aaccggagca attactaatc
     3481 gaagaggtta ttttatgagt gtttctcttt ccaaaggcgg gaacgtctcc ctgagtaaag
     3541 cagctccgtc aatgaaaaac gtcctggtgg gtcttggctg ggatgcacgt tcaactgacg
     3601 gtcaggactt tgacttggat gcttcagcat tcctgctgac cgcaaacggc aaagtgcgtg
     3661 gcgattcaga tttcatcttc tataacaacc tgacgtcatc cgacggttct gtaacgcaca
     3721 ccggcgataa ccgtactggt gagggggatg gtgatgatga atcgctgaaa attaaacttg
     3781 atactgtccc gtctgacatc gacaggatca tcttcgttgt gacgatccat gatgctcagg
     3841 ctcgccgtca gaactttggt caggtttccg gggcgtttat tcgcctggtt aatgacgata
     3901 accagactga agttgctcgc tacgatctga ctgaagatgc gtccactgaa accgccatgc
     3961 tgttcggtga actgtatcgt cacaatggtg agtggaaatt ccgtgctgta ggtcagggtt
     4021 atgcgggggg gctggcatct gtatgtgctc agtacggcat taacgcgtct tgatcgaaaa
     4081 ctaacttttc attccgggct gttgattcag ccctcattca actgtttgca ggagctaaaa
     4141 atggcagttt ctctcgtaaa aggcggcaac atatctctga ccaaagaagc accgaccatg
     4201 aatgttgcta tggttggtct tggctgggat gcccgtgtaa ctgacggtca ggcttttgat
     4261 ctggacgctt ccgtattcgc agtaggtgaa gacggtaaag tgctgtcaga tgcccatttc
     4321 attttcttca ataataaaac cagccctgat ggcgcagtag agcaccaggg tgacaaccgt
     4381 accggtgaag gcgatggtga cgatgagcaa attaaaatcg atctggcaaa agtctcagtt
     4441 gacgtcaaaa aactggtatt tgccgttacc atctatgatg cagaagcgcg taaacaaaat
     4501 ttcggcatgg tgagcaacag cttcatgcgc gtttacaaca acgataacgg cgcggaaatt
     4561 gcccgtttcg atctgtctga agacgcctca actgaaaccg caatggtctt cggtgaactg
     4621 tatcgccatg gcgctgagtg gaagtttaaa gctgtcggtc agggttttgc cggtggcctg
     4681 tcagcgcttg cttcccagca cggcgtcaac gtctaaaaga tgcttagtct gaccccggca
     4741 atgccgtaat gccgatcggt taaggatcag ttgaccgatc cagtggttgt gtaagaatcc
     4801 ggaaacgctc actcgtttcc ggatttttta tgcacactgg acaggccctt gatctggtat
     4861 cccgttacga ttctctgcgt aacccactgg cttctctggg ggattacctc gaccccgaac
     4921 tcatctctcg ttgccttgcc gaatcaggta cggtaacgct acgcaagcgc cgtcttcccc
     4981 tcgaaatgat ggtctggtgt attgttggca tggcgcttga gcgtaaagaa ccccttcacc
     5041 agattgtgaa tcgcctggac atcatgctgc cggcaatcgc cccttcgttg cccccagtgc
     5101 cgttattcag gcccgccagc acctgggaag tgaggctgtc cgccgcgtgt tcacgaaaac
     5161 agcgcagctc tggcataacg ccacgccgca tccgcactga tgcggcctga ccctgctggc
     5221 catcgatggt atgttctggc gcacaccgga tacaccagag aacgatgcag ccttcccccg
     5281 ccagatacat gccggcaacc cggcgctcta cccgcaggtc aaaatagtct gccagatgga
     5341 actgaccagc catctgctta cggctgagcc ttcggcacga tgaagaacag cgaaaatgag
     5401 cttgctgagc aacttataga acaaaccggc gataacaccc tgacgttaat ggataaaggt
     5461 tattactcac tgggactgtt aaatgcctgg agcctggtgg gagaacaccg ccactggatg
     5521 atccctctca gaaagggagc gcaatatgaa gagatcagaa aactgggtaa aggcgatcat
     5581 ctggtgaagc tgaaaaccag tccgcaggca cgaaaaaagt ggccgggact gggaaatgag
     5641 gtgacagccc gcctgctgac cgtgacgcgc aaaggagaag tctgccatct gctgacgtcg
     5701 atgacggacg ccatgcgctt ccccggggga gaaatggcgg atctgtacag tcatcgccgg
     5761 gaaattgaac tgggatacag ggagataaaa cagacgatgc aactgagcag gctgacgctg
     5821 taaaaagccg gagcttgtgg agcaagagct gtggggtgtc ttactggctt ataatctggt
     5881 aagatgtcag atgattaaaa tggtggaaca tctgaaaggt tactggccga atcaactgag
     5941 tttctcagaa tcatgcggaa tggtgatgac attgcagggc gcttcaccgg gacgtatacc
     6001 ggggctgatg cgcgatcttg caagtatggg acaacttgtg aaattaccga caagaaggga
     6061 aaggggcttc ccgagagtgg taaaggagag gccttggaaa gagcctgtac atagatttgt
     6121 gtaattgcct gattttgata tgttcaatca agcatcaaat gaaggttaat ttatggacga
     6181 aaaacagtta caggctctgg ctaacgaact ggccaaaaac ctcaaaaccc ctgaagacct
     6241 cagccagttt gaccgtctac tgaaaaagat cagcgttgaa gccgctctca atgcagagat
     6301 gacacaccat cttgggtatg agaaaaatca gtccaaaccg ggaactaact cccgcaacgg
     6361 ttattccaca aagaccgtta tcacaggcga cggtccactg gaactgcgta ctccgcgcga
     6421 tcgtgacggt accttcgaac cacaactggt aaagaaaaat cagaccctta ttactgggat
     6481 ggataaccag atcttctcgt tgtatgccaa agggatgacc acccgtgaga tcgctgctgc
     6541 gttcaaagag ctgtatgacg cagatgtttc accggcactg atatcaaagg tcaccgatgc
     6601 cgtgatggag caggttgtag aatggcaaaa ccgaccgtcg gatgctgttt accccattgt
     6661 ttatcttgac tgtatcgtcc ttaaagttcg gcaggacagt cgcgtcatta acaaatcggt
     6721 gttcctggca ctgggcatca atatcgaagg tcagaaagaa ctgccaggta tgtggctggc
     6781 cgaaaatgaa ggggcgaagt tctggctcaa tgtgctgact gaactgaaaa accgcggtct
     6841 gaacgatatc ctcatcgcct gtgtggatgg cctgaaaggc ttcccggggg ccatcaacac
     6901 agtatatccg aaggcccgca tccagttatg catcgtgcat atggtgcgca acagcctgcg
     6961 cttcgtgtca tggaaggact acaaagccgt cactcgaaac ctgaaagcga tttatcaggc
     7021 tcccacggaa gaggccggcc agcaggcaca ggaagcgttc gctgcgtcct gggacagtcg
     7081 ctatcctcat ataagccgaa gctggcaggc taactggccg aatcttgcca cgttcttcgc
     7141 ttatgcgacg ggcatccgca aagtgatcta tacgacgaat gccatcgagt cgctaaacag
     7201 cgtgatccgc catgcgatca aaaagcgtaa agtgttcccg acagacgact cggtgaaaaa
     7261 agtggtatgg ctggcaatcc agtcagcgtc ccagaaatgg acgatgccgt tgaaggactg
     7321 gcgtatggca atgagccgct ttattatcga cttcggtgac cgcctgggtg gtcacttctg
     7381 agaaagggca tttacacaga ttcctaaaca ggctccctgg aaatacccca cagccccgaa
     7441 aaagagccag tcagttgctg aactgactgg cattggctcc tggggcactt tattgttaat
     7501 cagtcactga tgtttc
//
LOCUS       DAZUCC010000219         7508 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZUCC010000219 DAZUCC010000000
VERSION     DAZUCC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.220"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            149..457
                     /locus_tag="ACHY09_004273"
     CDS             149..457
                     /locus_tag="ACHY09_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFS3887157.1"
                     /translation="MQSQTALLLLIIPFPVLVIASPESTQLALALKQLDGVKTSLEHA
                     RTSASNDPQARYFFDYLQAQQDISVVEQGIRRYLAPARAQPRAIQPLSANYQQENPDD
                     "
     gene            450..683
                     /locus_tag="ACHY09_004274"
     CDS             450..683
                     /locus_tag="ACHY09_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFS3887158.1"
                     /translation="MTDSQLAAFQAASLSKPETVSLLVLGLFTSVLLLWCAWTLMVAW
                     QGVCRQRLSWQSFGAVAAHSLLLVLVSFWLVLS"
     gene            693..1010
                     /locus_tag="ACHY09_004275"
     CDS             693..1010
                     /locus_tag="ACHY09_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFS3887159.1"
                     /translation="MRWFLLLLFATFNALADLPTMEDPSRGQGKGIMETLQNYGFDIV
                     VLLALAICAVGFLVVANNCIATYSEIQSGRKQWKDLGSMAGVGSILLVVIIWLLNQAS
                     DVL"
     gene            1012..1365
                     /locus_tag="ACHY09_004276"
     CDS             1012..1365
                     /locus_tag="ACHY09_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFS3887160.1"
                     /translation="MATIPFLPDRLNREPVVWRGLTTSELFLALALGLGGGCAFGILL
                     ALITHYWPLIPGSALAGAALLIQGGGRILARAKRGKPDTWLPQALLAWLERHHLRGAQ
                     LIQHSAVWVVRRSPR"
     gene            1362..2021
                     /locus_tag="ACHY09_004277"
     CDS             1362..2021
                     /locus_tag="ACHY09_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFS3887161.1"
                     /translation="MSRFRHALAERDNHILTLRAVIVLLCLCLAGVGYGWYSAPQKLT
                     LWFPPDLRSGSTRPWWEVPPASVYAFTFYIFQQLNRWPDNGEEDYSRNLRSLRAYLTP
                     ACYTRLEHDFQVRRNGGELRDRTRGVYELPGHGYSSLVHEGEKRPFPRVEALSRDSWR
                     VTLDVAVEEYYQNEPVKKALVRYPLQVVRYAIDAEKNPWGLAIDCTDAAPQRLVAEEK
                     P"
     gene            2018..2785
                     /locus_tag="ACHY09_004278"
     CDS             2018..2785
                     /locus_tag="ACHY09_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFS3887162.1"
                     /translation="MKPVLFTLLFSFPAFATEVVQWENIPLPVTLHVGQERIVDVGKP
                     VRIGYPATLEGKVRLQSAGGKVFLLANTAFPATRIQLRDTGNGELILLDIHATQGTST
                     LEPMQISYTPQTPATAKTSVPVTASTEPLPVLLVRYAAQNLYAPLRTVEALPGVTPAP
                     VRLAKRITTLLPQQPVTATPLASWQVNATTVTAIRLQNHSGQRITLDPRELQGQFTAA
                     AFQHDWLGPRGLAEDTTVAYLVTEGPVSRALLPEPKP"
     gene            2782..4077
                     /locus_tag="ACHY09_004279"
     CDS             2782..4077
                     /locus_tag="ACHY09_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFS3887163.1"
                     /translation="MMPRQSLLLKILLPLIVLVTVWLLLRPASKNTQETSLSRTVAAL
                     TPQELRDLGIDGDTPADTVATLVGQMKQYRRELQAIQTNNESQQAREKRLNAQQATLA
                     SQLREDMRRQQDQMRDELVKSQRGLLDKLDRLMAKRPDDIPPGLGLTPGELPADVQPD
                     IRWLEPLDAPPPVDEKLLRVPLKAADKPPPKPDVKVYTLPQNATLTGSVAMTALIGRV
                     PLNGTVSDPYPFKLLIGEDNLTANGVELPDVAGAVVSGIASGDWTLSCVRGQITSMTF
                     VFHDGIIRTVPQGGKSGNKQQEIGWLSDNQGLPCIPGERKSNAKEYLTSQFLLAGSGA
                     AAQAFANGESTTVVEDGSITSAVTGNNGQYVLGQAIGGGLKETADWMKQHYGQMFDAV
                     YVPPGHKVAVHLTEALSIDYEPEGRQVKYQQASTPGGLE"
     gene            4074..4433
                     /locus_tag="ACHY09_004280"
     CDS             4074..4433
                     /locus_tag="ACHY09_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012737281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFS3887164.1"
                     /translation="MKFTILILLLLLAGCSTSQEELMPVDPNASMLTLWQQQSGGNAA
                     LLDARAQLRRPPDGPVAQPEADTIRSQFPRLPNPDMVLYVFQHLNSNTPVPGYSTVFP
                     FYTRPQYALPGERLTPP"
     gene            4430..7072
                     /locus_tag="ACHY09_004281"
     CDS             4430..7072
                     /locus_tag="ACHY09_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFS3887165.1"
                     /translation="MNERRGLTEQALNTSYRKGPSFVDWLPWVEFLPESQTLLLEDGR
                     SVGAVFELYPVGTEGRSTAFLEQVRDTVTDALQDAFDEHERAPWVVQFFCQDEDDSEA
                     WLASLASYAAPEIRDSAFTREWLTQMANHMVNLTRDEGLFLDNTVTRTRWRATWRRTR
                     MVVYRWLPGLSPDEPSPEETLNNICERLTAGLKAAGISAIRQNEQDIHRWLVRWFNPA
                     PPAEDKKAFWQQLDLPETEPLPVLADFAERLLASEPRSDAANGVWWLDNRPHKVVVVD
                     RLRKAPPPGLLTGETARGDGINALVDLLPGGTIMNLTVVVYPQDKLEQHISRLADRAI
                     GDNTDSEYTRRDCSEVRSFLKDGHKLYRSTLAFYLSAPDTDILFRQQRQLQSLLLNAG
                     LVPVQEQHEVAPLSHWLRWLPMCFDPLRDPRGLYTRLNFVQHIAGLLPLYGRETGTGH
                     PGISYFNRGGTPLCFDPLNKLDRAQNGHLLLLGPTGAGKSATLNAKIAQLMALHRPRL
                     FIIEAGNSFGLMADYAAAHGLTVNKISLRPGSGVTLPLFADAWRLVEQAVTDAEPPED
                     EESDGDDQRDVLGEMEITARLMITGGEAKEDARLTRPDRAMIRQAIIAAAHTCYEEKR
                     QTLTQDIRDALFALSRDNSLPEARRQRAYEMAESINLFCAGFEGELFNREGQMWPEAD
                     ITLVDLATFAREGYEAQLAISYISLINHINNLGERAQHLARPIVNITDEAHIITVNPL
                     LARFLTKGSKMWRKLGIWLWLATQNLADFPDEAKKLLNMIEWWELLVMPPEEIEQVCR
                     FKALTEEQSQLLRSAVKSPGQYTEGVVLSPRIEALFRVVPPALYLALAMTEKHEKADR
                     MRIMKETGCSEVEAAITLSSVISQ"
     gene            7121..>7508
                     /locus_tag="ACHY09_004282"
     CDS             7121..>7508
                     /locus_tag="ACHY09_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887166.1"
                     /translation="MTAIACWFNREESDSIWAVSDSRITQQNSRLTDHCPKLFSIPVS
                     VIRSSDVYRIHPQKIFEFGFGFAGSTMIGINVKEMLAVSLARLHEIANNNPEQQISTE
                     AFPSLNEIASLAKNIAEQYMSDIGQSF"
BASE COUNT         1570 a         2299 c         2188 g         1451 t
ORIGIN      
        1 ttatctgaac caatttatta cgagaaaatc aaacagacca ttgacaactc tatgctaaaa
       61 attcttcatg tagtgaaatg attttttagc tgcttcacct ctgaagctct cctactttgc
      121 taatcattga gtataaggag agcttcaaat gcagtcccag acagcactat tactgttaat
      181 aatacctttc ccggtactgg taatcgcctc cccggaatcc acccagctcg ccctcgcgct
      241 gaagcaactc gacggcgtta aaacctcgct ggagcacgcc cggaccagcg ccagcaacga
      301 tccgcaggcg cgctacttct tcgactactt acaggcgcag caggacatct ctgtcgttga
      361 acaaggcatt cgccgctatc ttgccccggc tagagcacag ccgcgtgcta ttcagcccct
      421 gagcgccaac taccagcagg agaatcccga tgactgactc gcaactcgcc gcctttcagg
      481 ccgccagcct gagcaaaccg gagaccgtca gcctgctggt gctcggcctg tttaccagcg
      541 tcctgctgct gtggtgtgcc tggacgctaa tggtcgcctg gcagggcgtc tgccgccagc
      601 gtctgagctg gcagagcttt ggcgcggtgg cggcccattc actgttactg gtgctagtca
      661 gcttctggct ggtactgagc tgaggaggca tcatgcgctg gtttctgtta ctgctgttcg
      721 ccacatttaa cgccctcgcc gacctgccga cgatggaaga tccgtcacgc ggtcagggca
      781 agggcattat ggagacgctg caaaactacg gttttgacat cgtggtgctg ctggcgctgg
      841 ccatctgtgc cgtcggtttt ctggtagtgg cgaacaactg tattgctacc tattcggaga
      901 tccagagcgg acgcaagcag tggaaagatc tgggctcgat ggcaggcgtc ggcagcattc
      961 ttctggtggt catcatctgg ctgctcaacc aggcttcgga cgtcctgtga tatggcaacc
     1021 atcccctttc tccccgaccg cctcaaccgc gagcccgtgg tctggcgcgg cctcaccacc
     1081 agcgaactgt ttctggcgct ggctctcggt cttggcggcg gctgtgcgtt cggcattctg
     1141 ctggccctga taacccacta ctggccgttg atccccggtt cggcacttgc tggcgcggca
     1201 ttactgattc agggtggtgg gcggattctg gcccgcgcaa aacgcggtaa gcccgacacc
     1261 tggctgccgc aggcgctcct cgcctggctg gagcgtcatc atcttcgcgg cgcacaactg
     1321 atccagcaca gcgccgtgtg ggtggtgcgc aggagtccac gatgagccgc tttcgccatg
     1381 cgctggctga gcgcgataac catatcctga cgctgcgggc ggtgattgtt ctgctgtgcc
     1441 tgtgtctggc gggcgtcggc tacggctggt acagcgcgcc gcaaaagctc accctctggt
     1501 tcccgccgga tctgcgcagc ggcagcaccc gcccgtggtg ggaagtgcct cccgccagcg
     1561 tctacgcctt caccttttat atcttccagc agcttaaccg ctggccggat aacggcgagg
     1621 aggactattc ccgtaatctt cgttcgctgc gtgcctacct gacgcccgcc tgttacaccc
     1681 ggctggagca cgattttcag gtgcgtcgca acggcggtga actgcgcgac cgcacgcgcg
     1741 gcgtctatga actgcccggc cacggctaca gcagcctggt gcatgagggc gagaagcgcc
     1801 ccttcccgcg cgtggaggcg ctgagccggg attcatggcg ggtaacgctg gatgtggcgg
     1861 tggaagagta ctaccagaac gagccggtga aaaaagcgct ggtgcgctac ccgttgcagg
     1921 tggtgcgcta cgccatcgat gcggagaaaa atccgtgggg actggcgatc gactgtaccg
     1981 atgccgcgcc gcagcgtctg gtggcggagg agaaaccatg aagccagtcc tctttacttt
     2041 attgttcagt ttccccgcct tcgccacgga agtggtgcag tgggaaaaca tcccgctgcc
     2101 cgtcacgctg catgtcgggc aggagcgaat tgttgacgtg ggtaaaccgg tgcgcattgg
     2161 ctatcccgcc acgctggaag gcaaagtgcg tctgcaaagc gcaggcggca aggtcttcct
     2221 gctggcgaac acggcgttcc cggccacccg catccagtta cgtgataccg gcaacggtga
     2281 gctgatcctg ctggatattc atgccaccca gggcacttcg acacttgaac caatgcaaat
     2341 tagctatacg ccgcagacgc ctgccacagc aaaaacatcc gtcccggtga cagcatcaac
     2401 agaacctctg cccgtcttgc tggtgcgcta cgccgcgcaa aatctgtatg cgccgctgcg
     2461 caccgtcgaa gccctgcccg gcgtcacacc tgcgcccgtc cggctggcga aacgcatcac
     2521 cactttactg ccgcagcagc cggttaccgc caccccgctg gcgtcatggc aggtcaacgc
     2581 caccaccgtc accgccatcc gcctgcaaaa tcacagcgga cagcgcatca cgctcgatcc
     2641 gcgcgagctt caggggcagt tcaccgctgc cgcgttccag catgactggc tcggcccgcg
     2701 tggtctggca gaagatacca ccgtggcgta cctggtgacg gaaggtccgg tttcccgcgc
     2761 cctgctgccg gagcccaaac catgatgccg cgccagagcc tgctgctgaa aatcctgctg
     2821 ccgctcatcg tactggtaac ggtatggctg ctgctgcgcc cggccagcaa aaacacgcag
     2881 gagacttccc tgtcccgcac cgtggcggcc ttaacgccgc aggaactgcg cgatctgggc
     2941 atcgacggcg acacgcccgc cgacaccgtc gccacgctgg tcggccagat gaaacagtac
     3001 cgccgcgaac ttcaggcaat ccagacaaac aacgaaagcc agcaggcgcg cgaaaaacgg
     3061 cttaacgcac agcaggccac gctggcctct cagcttcggg aagacatgcg ccgccagcag
     3121 gaccagatgc gcgatgaact ggtgaaaagc cagcgcgggc tgctggataa gctcgaccgt
     3181 ctgatggcca agcgcccgga cgatatcccg cccggtctgg ggctgacgcc cggcgaacta
     3241 cctgccgacg ttcaacccga tatccgctgg ctggagccgc tggatgcgcc tccacctgtg
     3301 gatgaaaagc tgctgcgcgt ccctctgaaa gcggccgaca aaccaccgcc gaaacctgac
     3361 gtgaaggtct acaccctgcc gcagaacgcc acgctgaccg gatcggtggc gatgacggcg
     3421 ctgattggcc gcgtgccgct taacggcacc gtcagcgatc cctacccgtt taaacttctg
     3481 attggcgagg acaacctcac cgccaatggc gttgagctgc cggacgtggc gggcgcggtg
     3541 gtctccggca tcgcctcggg agactggacg ctctcctgtg tgcgcgggca aatcacctcg
     3601 atgacctttg tgtttcacga cggcatcatc cgcaccgtcc cgcagggcgg caaatccggc
     3661 aacaaacagc aggagatcgg ctggctttcg gataaccagg ggctgccatg tattcccggc
     3721 gagcgtaaaa gcaatgcgaa ggagtacctg acctcgcagt tcctgctggc gggttccggt
     3781 gcggcggcgc aggccttcgc caatggcgaa agcaccacag tggtcgagga cggcagcatc
     3841 accagcgcag tgacgggcaa caacggtcag tacgtgctcg gccaggctat tggcggcggg
     3901 ctgaaagaga ccgccgactg gatgaaacag cactacggcc agatgttcga cgccgtgtac
     3961 gtgccgccgg gccacaaggt ggcggtgcat ctcaccgaag cgctgtctat cgactacgag
     4021 ccggaaggcc gtcaggtgaa ataccagcag gccagcacac cgggaggtct ggaatgaaat
     4081 ttaccatcct tatacttctg ttgttactgg ccggatgcag cacctcgcag gaggaactga
     4141 tgcctgtcga ccccaatgcc agcatgctga cgctgtggca gcagcaaagc ggcggcaacg
     4201 cggctctgct ggatgcccgc gcccagctac gccgcccgcc agatggtccg gtggcgcagc
     4261 ctgaggcaga cactatccgc agccagtttc cgcgcctgcc caacccggat atggtgcttt
     4321 acgtcttcca gcacctcaac agcaacacgc cggtacccgg ctacagcacc gttttcccct
     4381 tctatacccg tccgcagtat gcgttgccgg gcgaacgcct gacgccgcca tgaatgaacg
     4441 gcgcggcctg actgaacagg cgctgaatac cagctaccgc aagggcccgt catttgtcga
     4501 ctggctgccg tgggtggagt ttttgccgga gtcgcagacg ctgctgctgg aagatggccg
     4561 ctcggtaggt gccgtatttg agctatatcc cgttggcacc gaaggccgct caacggcgtt
     4621 tctggaacag gtccgcgata ccgtcaccga tgccttacag gatgcgtttg atgagcatga
     4681 gcgcgcaccg tgggtggtac agtttttctg tcaggatgag gacgacagcg aggcgtggct
     4741 ggcatcactt gcctcttacg ccgcaccgga aatccgcgac tccgccttta cccgcgagtg
     4801 gctcacacag atggcaaacc atatggtgaa tctcacccgc gacgagggac tgtttctcga
     4861 taacaccgtc acccgtaccc gctggcgggc gacgtggcgg cgcacgcgga tggtggtgta
     4921 ccgatggctt cctggacttt caccggatga accgtcgccg gaagagacgc tgaataatat
     4981 ctgcgaacgc cttaccgccg ggctaaaagc ggcgggtatc agcgctatcc gccagaatga
     5041 gcaggacatt caccgctggc tggtgcgctg gtttaatccc gcgccgcctg ctgaggacaa
     5101 aaaagccttc tggcagcagc ttgacctgcc ggaaactgaa ccgctgccgg tcctggccga
     5161 tttcgccgaa cgcctactgg cgagtgaacc ccgttcggat gcggcaaacg gcgtctggtg
     5221 gctggataac cgcccgcata aagtggtggt ggttgaccgc ctgcgcaaag cgccgccgcc
     5281 cggcctgctg accggggaaa ccgcgcgcgg agacggcatt aacgccctgg tcgacctgct
     5341 gcctggcgga accatcatga acctgaccgt ggtggtgtat ccgcaggaca agctggagca
     5401 acacatcagc cgcctggccg accgggcgat tggcgacaat accgattcgg aatacacccg
     5461 ccgcgactgc agcgaggtgc gaagcttcct gaaggacggc cataaactct accgcagcac
     5521 gctggcgttt tatctcagcg cgccggacac cgatatcctg ttccgccagc agcgtcagct
     5581 acagagcctg ctgctcaacg ccgggctggt tccggtgcag gagcagcacg aagtcgcccc
     5641 cttaagccac tggctgcgct ggctgccgat gtgcttcgat cccctgcgcg acccgcgcgg
     5701 gctgtatacc cggctcaact ttgtgcagca tatcgccggg ctgctgccgc tgtacggacg
     5761 ggaaaccggc accgggcatc cggggatcag ctacttcaac cggggcggca cgccgctgtg
     5821 cttcgatccg ctgaacaagc tggatcgtgc ccagaacgga caccttttac ttcttgggcc
     5881 aaccggagcg ggcaaatccg ccaccctcaa cgcgaaaatc gcgcaactga tggcgctaca
     5941 tcgtccgcgc ctgtttatca tcgaggccgg aaactcattt ggcctgatgg ccgactacgc
     6001 cgccgcccac ggcctgacgg tgaataaaat cagcctgcgc cccggttctg gcgtcaccct
     6061 gcccctgttc gccgatgcgt ggcgactggt ggaacaggca gttaccgacg ccgaaccgcc
     6121 agaggacgaa gagagcgacg gcgatgacca gcgcgacgtg ctgggtgaaa tggagatcac
     6181 cgcccggttg atgattaccg gtggtgaagc gaaagaggat gcgcgcctca cccggccgga
     6241 tcgggcgatg atccgccagg cgattatcgc tgccgcgcac acctgctacg aggaaaaacg
     6301 acagacgctg acccaggata tccgtgacgc actgttcgcc ctttcccgcg acaacagtct
     6361 gccggaagcc cgccgccaga gggcgtatga aatggcggaa tccatcaacc tgttctgcgc
     6421 cgggtttgaa ggcgaactgt ttaaccgtga aggccagatg tggccggagg cggatatcac
     6481 cctagtggat ctcgccacct ttgcccgcga aggctatgaa gcgcagcttg ccatcagcta
     6541 tatctcgctg attaaccata tcaataacct cggcgagcgc gcccagcatc tggcgaggcc
     6601 tatcgtcaat atcaccgatg aggcgcatat catcactgtt aatcccttat tggcgcgctt
     6661 tctcaccaaa ggctcgaaga tgtggcgcaa gctcggtatc tggctgtggc tcgccaccca
     6721 gaacctggcg gattttcctg atgaggcgaa gaagctgctc aatatgatcg aatggtggga
     6781 actactggtg atgccgccgg aagagatcga gcaggtctgc cggtttaaag cgcttacgga
     6841 agaacagagc caactgctgc gctcagcggt aaaatcaccg gggcagtata ccgagggcgt
     6901 ggtgctttca ccgcgtatag aggcactgtt tcgggtggtg ccgccagcgc tctatctggc
     6961 gctggcgatg acagagaaac atgaaaaggc cgataggatg cggattatga aggagacagg
     7021 gtgtagtgaa gtggaggcgg cgataaccct aagctcggta atctcgcaat aaaaatatgc
     7081 taagctattc gcattaatgt taaataactg gaaaaacctt atgaccgcaa ttgcatgttg
     7141 gtttaataga gaggaaagcg atagtatttg ggcagtcagc gactcaagaa tcactcagca
     7201 aaatagcaga ctaactgacc attgtccgaa attattctca atccccgtat cagttattcg
     7261 ctcttcggac gtttatcgaa ttcacccgca aaaaatattc gaatttggct tcggatttgc
     7321 aggaagtaca atgattggaa ttaatgtcaa agaaatgctt gcagtatcat tagcaagact
     7381 tcatgagatt gcgaacaata atccagaaca acaaatatcg accgaagcat tcccaagtct
     7441 taatgaaata gcttcattag caaaaaatat tgccgagcaa tatatgtctg atatcggcca
     7501 atcctttc
//
LOCUS       DAZUCC010000220         7490 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZUCC010000220 DAZUCC010000000
VERSION     DAZUCC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.221"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            192..4442
                     /gene="fdeC"
                     /locus_tag="ACHY09_004283"
     CDS             192..4442
                     /gene="fdeC"
                     /locus_tag="ACHY09_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFS3887167.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAQPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGITVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEALVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPAKLVLDPNGTATTNEQGQA
                     IFTATTTVAATYTLTAQVSQNNGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4567..5424)
                     /locus_tag="ACHY09_004284"
     CDS             complement(4567..5424)
                     /locus_tag="ACHY09_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3887168.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKSFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARMNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5673..6542
                     /locus_tag="ACHY09_004285"
     CDS             5673..6542
                     /locus_tag="ACHY09_004285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS3887169.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6702..7295)
                     /gene="rclC"
                     /locus_tag="ACHY09_004286"
     CDS             complement(6702..7295)
                     /gene="rclC"
                     /locus_tag="ACHY09_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFS3887170.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIIFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(7307..>7490)
                     /gene="rclB"
                     /locus_tag="ACHY09_004287"
     CDS             complement(7307..>7490)
                     /gene="rclB"
                     /locus_tag="ACHY09_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFS3887171.1"
                     /translation="STNADDKIILKHISVSSVSASPTVLENAIADMARKYNASSWKVT
                     SMRIDNNSTATAVLYK"
BASE COUNT         2038 a         1665 c         1932 g         1855 t
ORIGIN      
        1 atcccgcgcc caaccacctg atgagtggct acagacactg gatatattag gtggcggtgt
       61 actttcttac ataaaggtat ttccttttct gtggaaaagg aaaccgggaa atccccggtt
      121 tttctgacaa gcagacgcca ttatttgtgt ctgcctatgt tcgttaattc gttcatcagg
      181 aaattatctc aatgtcacgt tataaaacag acaataaaca gccacgattt cgttattcag
      241 ttctggcccg ctgcgtggcg tgggcaaata tctctgttca ggttcttttt ccactcgcag
      301 tcacctttac gccagtaatg gcagcacgtg cgcagcatgc ggttcagcca cggttgagca
      361 tggaaaatac tacggtaact gctgataata acgtggagaa aaatgtcgcg tcgcttgccg
      421 ctaatgccgg gacattttta agcagtcagc cagatagcga tgcgacacgt aactttatta
      481 ccggaatggc caccgctaaa gctaaccagg aaattcagga gtggctcggg aaatacggta
      541 ctgcgcgcgt caaactgaat gtcgataaaa atttctcgct gaaggactct tcgctggaaa
      601 tgctttatcc gatttatgat acaccgacaa atatgttgtt cactcagggg gcaatacatc
      661 gtaccgacga tcgtactcag tcaaatattg gttttggctg gcgtcatttt tcagaaaatg
      721 actggatggc gggggtgaat acttttatcg atcatgattt atcccgtagt catacccgca
      781 ttggtgttgg tgcggaatac tggcgtgatt atttgaaact gagcgccaat ggttatatcc
      841 gggcttctgg ctggaaaaaa tcgccggatg ttgaggatta tcaggaacgc ccggcgaatg
      901 gctgggatat tcgtgctgag ggctatttac ctgcctggcc gcagcttggc gcaagcctga
      961 tgtatgaaca gtattatggc gatgaagtcg ggctgtttgg taaagataaa cgccagaaag
     1021 atccacatgc gattaccgct gaagtgaatt acacgccagt gcctcttctg accctgagtg
     1081 ccgggcataa gcagggcaag agtggtgaga atgacactcg ctttggcctg gaagttaatt
     1141 atcggattgg cgaacctctg gaaaaacaac tcgatacaga cagcattcgc gagcgtcgaa
     1201 tgctggcagg cagccgctat gacctggttg agcgtaataa caatatcgtt cttgagtatc
     1261 gcaaatctga agtgatccgt attgctctgc ctgagcgtat tgaaggcaag ggcggccaga
     1321 cggtttccct ggggcttgtg gtcagcaaag caactcacgg tctgaaaaat gtgcaatggg
     1381 aagcgccgtc tttgctggcc gcaggcggaa aaattacggg gcagggcaat cagtggcaag
     1441 tgacgctccc ggcttatcag gcaggcaaag acaattatta tgcgatttca gcgattgcct
     1501 acgataacaa aggcaatgcc tcgaaacgtg tgcagacaga agtggttatt agcggagctg
     1561 gtatgagcgc cgatcgtacg gcgttaacgc ttgacggtca gagccgtatt caaatgcttg
     1621 ctaacggtaa tgagcaaaag ccgttggtgc tgtctctgcg cgacgccgag ggtcagccag
     1681 tcacgggcat gaaagatcag atcaagactg aactaacctt caaaccggct ggaaatattg
     1741 tgactcgtac cctgaaggcc actaaatcac aggcacagcc aacactgggt gagttcaccg
     1801 aaactgaagc aggggtgtat cagtctgtct ttactaccgg aacgcagtca ggtgaggcaa
     1861 cgattactgt tagcgttgat gacatgagca aaactgtcac tgcagaactg cgggccacga
     1921 tgatggatgt ggcaaactcc accctgagcg ctaacgagcc gtcaggtgat gtggttgctg
     1981 atggtcagca agcctacacg ctgacactga cagcggtgga ctccgagggt aatccggtga
     2041 cgggagaagc cagccgcctg cgacttgttc cgcaagacac taatggtata accgttggtg
     2101 ccatttcgga aataaaacca ggggtttaca gcgccacggt ttcttcgacc cgtgccggaa
     2161 acgttgttgt gcgtgccttc agcgagcagt atcagctggg cacattacaa caaacgctga
     2221 agtttgttgc cgggccgctt gatgcagcac attcgtccat caccctgaat cctgataaac
     2281 cggtggttgg cggtacagtt acggcaatct ggacggcaaa agatgctaat gacaaccctg
     2341 taactggcct caatccggat gcaccgtcat tatcgggcgc agctgctgct ggttctacgg
     2401 catcaggctg gacggataat ggcgacggga cctggactgc gcagatttct ctcggcacta
     2461 cggcgggtga attagacgtt atgccgaagc tcaatgggca ggacgcggca gcaaatgcgg
     2521 caaaagtaac cgtggtggct gatgcattat cttcaaacca gtcgaaagtc tctgtcgcag
     2581 aagatcacgt aaaagccggt gaaagcacaa ccgtaacgct ggtggcgaaa gatgcgcatg
     2641 gcaacgctat cagtggtctt tcgttgtcgg caagtttgac ggggaccgcc tctgaagggg
     2701 cgaccgtttc cagttggacc gaaaaaggtg acggttccta tgttgctacg ttaactacag
     2761 gcggaaagac gggcgagctt cgtgtcatgc cgctcttcaa cggccagcct gcagccaccg
     2821 aagccgcgca gctgactgtt attgccggag agatgtcatc agcgaactct acgcttgttg
     2881 cggacaataa aactccaacg gttaaaacga cgacggaact caccttcacc gtgaaggatg
     2941 cgtacgggaa tccggtcacc gggctgaagc cagatgcacc agtgtttagt ggtgccgcca
     3001 gcacggggag tgagcgtcct tcagcaggaa actggacaga gaaaggtaat ggggtctacg
     3061 tgtcgacctt aacgctggga tctgccgcgg gtcagttgtc tgtgatgccg cgagtgaacg
     3121 gccaaaatgc cgttgctcag ccactggtgc tgaatgttgc aggtgacgca tctaaggctg
     3181 agattcgtga tatgacagtg aaggttaata accaactggc taatggacag tctgctaacc
     3241 agataaccct gaccgtcgtg gacagctatg gtaacccgtt gcaggggcaa gaagttacgc
     3301 tgactttacc gcagggtgtg actagcaaga cggggaatac agtaacaacc aatgcggcag
     3361 gtaaagtgga cattgagctt atgtcaacgg ttgcagggga acttgagatc gaggccttgg
     3421 tgaaaaactc tcagaagacg gtcaaggtga aattcaaggc ggatttcagt accggtcagg
     3481 cgagcctgga ggtagacgcc gctgctcaaa aagtggcaaa cggcaaagat gcctttacgc
     3541 tgacggcaac ggttaaggat caatacggca accttcttcc tggcgctgtg gtcgtcttta
     3601 atctgcctcg gggcgtcaaa ccgcttgcag acggtaatat catggtgaac gccgacaagg
     3661 agggtaaagc ggaactgaaa gtggtttccg tgactgccgg aacctatgag atcacggcgt
     3721 cagcaggaaa tgaccagcct tcgaatgcgc agtctgtaac gtttgtggct gataagacta
     3781 cggcgaccat ctccagtatt gaggtgattg gcaaccgtgc agtggcggac ggcaaaacca
     3841 aacagacgta taaagttacg gtgactgatg ccaataacaa cctgctgaaa gatagcgaag
     3901 tgacgctgac tgccagcccg gcgaagttag ttctggatcc caatgggacg gcgacaacga
     3961 atgagcaagg gcaggctatt ttcaccgcca ccacaactgt tgcggcgaca tatacactca
     4021 cggcgcaagt gagtcagaac aacggtcagg tatcaacgaa aaccgccgaa tctaaattcg
     4081 tcgcggatga taaaaacgcg gtgctcgctg catctccaga gcgtgtagat tctctggtgg
     4141 cggacgggaa gactactgca acactgacgg ttactctgat gtcgggtgtc aaccccgtag
     4201 gaggaaccat gtgggtcgac attgaggctc cggaaggggt gacagaggcg gattatcagt
     4261 tcctgccgtc gaaaaatgac catttcgcga gcgggaaaat cacgcgtaca tttagtacca
     4321 acaagccagg tacatacaca ttcacattca actctttgac atatggaggg tatgaaatga
     4381 aaccagtgac tgtgacaatt aacgccgttc ctgcagatac tgaaggcgct gaggagaaat
     4441 aaaaacgacg atgtaataaa taataatggc gttgacatgc gcagcgccat tatttactga
     4501 atacctaccc tggcaccgat tagacggtgg cagggtaggg taaatcttat gtgatggagt
     4561 tacaggctaa accgggcgaa cggggataaa aatttcgcaa acaatgttat tgctatcaat
     4621 ttcgacgaag ttttcattgt aataaaatcg ctcaatatca ctgccgttca ttctggctaa
     4681 ccccctggca ggtaattcca tcagatagag gtttcgcgcc cattcagggt agtgctccca
     4741 ctcaccttca tagcggaaag aggcatacag tcctcggtta attgtccact ctttcgacat
     4801 ctctgaatcg gtttgtttgc ataccgtacc ggcaaaagtt tcaactgtga gcaaatcgac
     4861 cgattttgtt gatggttcga acgtggagac gaaatagata tccgtcttaa aactcttcat
     4921 acatctggct aaatgatgac gtcgcagttt gacttcttca tccggtgatt tattcgacac
     4981 ttcaatatca tatttaaatg aattaccaaa aataggtgtc tcaggaaagt tgactaatct
     5041 acactccgtc ttttgctgtt gacgtattaa aaaagaaggg aagatatttg cgagatccca
     5101 ataatcacgg tggcggtatt cacggggaga gcagccgaat aactttttaa attcgcggct
     5161 gaatgactgc tgtgagtcaa aatgcaaaga gagtgcaata tcaagcatag atttcgcgga
     5221 gagccggaca agaatggcgg cacgacaaag ccttcgtttg cgaatgtatt ctcccaaagg
     5281 cacatgcatg aaatttcgga acagaagctg gatgttgcgt ctgctgtagc cagatttctg
     5341 tgcgatatct tcgattgaaa tagggtgttc gagattgcac tcaatccact ccaggagctg
     5401 ctgtagaatc ttctgcctga tcatcacatt ctccgttaat tctttgtcat atcgcacatt
     5461 ttcgttatgc cctgggccct aagtgtatca aaaacagcaa gggcgaaggc aaaagcactg
     5521 gtagcgtctg cttcgttgtc cccgaaaata gagaggtttc agtcctacat tattaatgaa
     5581 ttttttgcat aagtgatatc aaaatccaca tactaatttg aggttacgtt ttaacgtaga
     5641 ctcattgttc atgcctaatg gagggactga cagtggaatt ttccgtatta agtaacaatc
     5701 tgaaaatgcc gatgatggga tttggtgttt ttcaggttac cgataaaaac gtgtgcaaac
     5761 agtcagtgtt gaacgctatc cgcacgggtt atcgactcat cgacactgca gcggtatacg
     5821 gtaatgaaga tgccgtcggt gaagccgttc gtgaagctat atctgaaggt ttatgtaccc
     5881 gagaagagtt atttataacg tctaaattgt gggtgcagga tatgttgaat caggatattg
     5941 cagcagcagg tattgaagca tcattaaaaa aatcgggact agaatacttc gatctctatt
     6001 tattgcacca ggccatgcgc gattatttta gcgcgtggcg tgcactcgaa gatgcctatg
     6061 aagagggcaa attaaaagcg attggggttt ccaatttcta ccctcatgtt ctggcgaact
     6121 tttgtgaaac ggtaagagtt aaaccgatgg tcaaccaggt cgagttgcat ccctattttg
     6181 cccaaccaga ggcgctggca accatgaagt attataacgt gcagcctgaa gcatgggctc
     6241 cgttaggtgg tggacgacat aaaccctttg aaaataatct gcttcagagt attgcagatg
     6301 cccatcaaaa atcgatttct caggtcattc tgcgttggaa tattcaacgg ggagtggtcg
     6361 ttattccgaa atcgacacat caacagcgta tcgaagaaaa ttttgctatc tgggatttct
     6421 cactgacaga gaaagaaatg gcgcaaatta gttcgcttga tttgggttat gttggggaat
     6481 cggtaaaaca ttttaatcct gagtttgttc gtggttgtct tgctgtaaaa atacatgatt
     6541 gatattaatc acttatttta cctgagacga caagaatctt ttaacagggg agtgatattg
     6601 atcttcactc tgtcatatct ccggtaatat ggcgtcaggc ttcatcatca gaaagggggc
     6661 ctgacgcctg aaaaagtgaa caacagacag tgttcggatt atcaatattc agtttttaac
     6721 gttgaactgg attcattact gcgttgttta agaatatccc gcgccgaatc tgccattatc
     6781 atgacagcac ctgccagcat cagagtatct ttcaatacca gacgaccagc accggataaa
     6841 taagggaaac catgatgagc gtcacccaat gcgggtaccc atgcctccgg ggtggtgatt
     6901 aaaaatgaga gtgttaccag cggcgtggta aatgccatca gcccacccaa taaacctaac
     6961 cagcgattga caggattagc caaaaccagc agagcaataa tcacctccac gacgccaaga
     7021 ccgttggaaa aaccataggt attattagcc gtttgccatg ctcttgcttc tggtttgtat
     7081 tcgccttcgt gagtcagatg ctgtttatag tcttccgggt gttcataaaa gaacgacatt
     7141 agtggactgt ttgcgacgaa tggtgtaatg ctgtctgcct cgtaagggac aaactttaat
     7201 aacccaatcc acataaaaat aatcgcaata ctcagacgaa tcaatgtcag gccaatttta
     7261 tctccccgac ttaacaggtg caggtatctt tccattgtaa acatccttat ttatacaata
     7321 ctgctgttgc ggttgaatta ttatcaattc gcatcgatgt aactttccag gatgaagcat
     7381 tatattttct ggccatatca gcaatggcat tctcaagaac tgtcggtgat gctgatactg
     7441 atgagacgct gatatgtttc agaattattt tatcatctgc attggtggaa
//
LOCUS       DAZUCC010000221         7455 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZUCC010000221 DAZUCC010000000
VERSION     DAZUCC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.222"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..455
                     /locus_tag="ACHY09_004288"
     CDS             204..455
                     /locus_tag="ACHY09_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS3887172.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(486..680)
                     /locus_tag="ACHY09_004289"
     CDS             complement(486..680)
                     /locus_tag="ACHY09_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887173.1"
                     /translation="MTIAERLIQKGFDEGFKQGFKQGALEVAREIACRLRDIGWPPER
                     IQEATGLSGEELKKLFPDEQ"
     gene            complement(742..1704)
                     /locus_tag="ACHY09_004290"
     CDS             complement(742..1704)
                     /locus_tag="ACHY09_004290"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFS3887174.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPASFVEPDLHQYASDILWSVKTTGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQYKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQQGIQ
                     QGIQKGRMEEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
     gene            complement(1701..2090)
                     /locus_tag="ACHY09_004291"
     CDS             complement(1701..2090)
                     /locus_tag="ACHY09_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFS3887175.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            complement(2280..2546)
                     /locus_tag="ACHY09_004292"
     CDS             complement(2280..2546)
                     /locus_tag="ACHY09_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887176.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSERKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(2639..3073)
                     /locus_tag="ACHY09_004293"
     CDS             complement(2639..3073)
                     /locus_tag="ACHY09_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFS3887177.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            complement(3803..4303)
                     /locus_tag="ACHY09_004294"
     CDS             complement(3803..4303)
                     /locus_tag="ACHY09_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS3887178.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(4764..5360)
                     /locus_tag="ACHY09_004295"
     CDS             complement(4764..5360)
                     /locus_tag="ACHY09_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887179.1"
                     /translation="MMKSDEKYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVRAAQDWLCDRKLVDITDILR"
     gene            complement(5357..6076)
                     /locus_tag="ACHY09_004296"
     CDS             complement(5357..6076)
                     /locus_tag="ACHY09_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFS3887180.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGELCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENTI"
     gene            complement(6073..6507)
                     /gene="psiB"
                     /locus_tag="ACHY09_004297"
     CDS             complement(6073..6507)
                     /gene="psiB"
                     /locus_tag="ACHY09_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFS3887181.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(6562..>7455)
                     /locus_tag="ACHY09_004298"
     CDS             complement(6562..>7455)
                     /locus_tag="ACHY09_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887182.1"
                     /translation="LLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNVCVQRGVQ
                     LRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPDKSLALLA
                     WTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARLAALLPEG
                     WSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAIGFHMRDW
                     WQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKDNRWVPGW
                     MCTPRPQMDATEHTTNLADAA"
BASE COUNT         1679 a         2178 c         1978 g         1620 t
ORIGIN      
        1 gaagaaaaac gacggcacaa caatggcatg tcgtggtcac tggtgcacca ctcccgcaca
       61 ggtgaaaaca gagaaaacaa cacgacatat acaatcaaaa aaaagtgaaa tttcttcact
      121 ttttcactct tttttatcgc gctgacttgg tgactatgat gataaaataa agtgaagttt
      181 tttcactttt tcaaaggtgt tttatgaccg gctgggaact gcgaatctgg cgaaaaagca
      241 tgctctggtc acgcgaaaaa gcggccagag aatttggcgt gacacagaga acatggcacg
      301 cctgggaaaa tgcggaacag gtggatgtca ccgtctggag gaccacccag gcactttcag
      361 tccgtgacct gctgcctcac atgcagggga tgcgtaaggc agatatcatc aggcgactgg
      421 aaaatgaact gggggaaacg gcagaggatg tgtgagcgtg attcacagcc tggctgaatg
      481 ccaggctact gctcatcagg aaacagcttt ttcagttctt caccggaaag tccggttgcc
      541 tcctgaatcc gttccggcgg ccagcctata tcccgaagcc ggcaggctat ttcccgcgct
      601 acttcaagag ccccttgttt aaaaccttgt ttaaaaccct catcgaaacc tttctgtata
      661 aggcgttctg caatcgtcat gctgtttgct ctgctcctgc tgacaaaaaa cccggcattt
      721 tgccgggctt tgtggttctc atcagtgagt gatgttcttc agttcagctt cggaaagtcc
      781 ggtgatctga cagatttgtg cagatggcat tcccgttttc tgaagctgac gcgcaatatc
      841 ctgcttctcc tccatgcggc ctttctgtat tccctgctgt attccctgct gtattccctg
      901 ctgtattccc tgctgtattc cctgctgtat tcctttctgt attccttgct gctcaagacg
      961 ttcggcaata gtcatcagtt tatcctcatg ctgcggcaga cgctcagcca gcgcccgcat
     1021 aaattccggt gatacagagt cccctgcgtt aacgatgtaa tgaatcagaa cacgcacctg
     1081 ttcatctgaa atttccacca tgacctgcgg gagtttgtcc agcagctcca tcatgtcacg
     1141 atggcggata tgcttcatca gcagcgtcag tgcggccatc cggcgatggt tcatgatttc
     1201 attgtcatca acgacagtga tatcaaccag cggaaacggc tttgtgtata ttttagccgc
     1261 aagcgccgga tcctcaaaac agtccagcca gttcatgctg tacgggtacg ggctgcgctc
     1321 accatgatag aacagcaccg gaatcaccag tggcaatgtt ttgtactgct ccagatgtct
     1381 ctgcatcgcc gccacactgt aacgtaacat gcggaaaggc atgtacagat tttcggtgct
     1441 ctggtgctcg atgagcgtat aaacatagcc gtctttaccc ccggtggttt tcacactcca
     1501 gaggatatcg ctggcgtact gatgcaggtc cggctcaaca aaggaggcgg gttccagctt
     1561 cagcgtggac aggtcgcaca actgccggta ctccgccgga agatgcagtt ccagaaaatc
     1621 tctggcgacg tcggggttcg ccaggaacga ccggaacgcg gcatcatgcg gcgtgggcgt
     1681 tgtggtgttt ttcttcttac tcacggttcc tccatcaaat cagcagcatc gcagcaatca
     1741 tcgtccaaat ccccacaacc aagaggacag caattgcgat caaagccgtg acaacaatag
     1801 ccacgttctg cgtcacggcg tatttcttct gcaatctctt cagcggaaag gtaatcaaac
     1861 tcctcacggg ccagttccag gaccagcaga tgttgttctg tcggcaatgt agccacaaaa
     1921 ttatcaatag cagcactcaa acgtcgctga tatagctcat aaacaagacc acaacggtgc
     1981 attagttctt cggataatag ttccagtttt gagcgaacgc tgtcagcatt gaaagtgact
     2041 tcatcagatg acatctgaaa atctttgtca gtgtcaaaac aagacttcat ttttatctcc
     2101 tgcccgtccg ggccctgaat gaaagagaac ctgtttccct tctctgtttt ttatggaaca
     2161 ggaggaatcc ggtaccatgc gacatagacg tcatgatgtt tttcatcttg ctcacggttc
     2221 ctcctggtaa agtacctgaa catatcctgc cacctgcaca gacagacggc agggggtaat
     2281 cagtggcaga cccgttcaat ttcaagcgca cggagcaggt gcccccactc gctgactttc
     2341 atggcgggga tacgcacccg cttccgctca ctgcagcgtc tggcgatatg accgcccatc
     2401 gccaggacgt ccgggttaat ctcatccggt gccagttcct ggcgtctgaa gcgggtatgg
     2461 cggtgcaggg gtttgttctc ccgcaggcac tcttcacgca gacgctttac atactcggcg
     2521 gttgcgaaga taattgatgg tctcatggca taatctccat agttagtgaa tgagatgccg
     2581 gacttccgta cggggaagcg ccggcggaga aaggcagggt ttatcgccct gctttttttc
     2641 attccggaca gcgacgcagt gccagcgcga gcgctttcat cttccagcct tccggcgtgc
     2701 ggtacatcac cttccagtag ccgtcgatga acacataatg gaagcgcacc tcttctgtgc
     2761 tttccgtggc aatagcctcc atgaaagaac ggtactcacg cgcctcactg tccatccgga
     2821 aaccactgtc acgtccgtaa taagccgtca caccgtccac cgggttctga agcgtatgcc
     2881 cggcgggttt gtcgcaacgg ggaccgaggc aacggatatc accgtgttca accagggctt
     2941 cggcctgctg accggtggca tagtgggtat gcaggatgcg accgttccag gtcggctcac
     3001 cgtgcaggtg gcagtaaacg gtacggaaca ccccgtcgcg gcaaaaaacg gaaatcgtgc
     3061 tgctgactgt catgaaaacg tctccttctg ctgaatcagg tcgccgggcc gaccggttgc
     3121 tcccccggag gggcgaaagc cccttctgcc gtccacttct tcggggtttc tccccgcagt
     3181 ggacctttcc gatggggcag cgacagccac ggcacggggc agttgtccgg gggcagacgc
     3241 aaaagttttt gacgcttgcg gcaaaaagtt ttgtggctgg tacatctccc cctggacggc
     3301 tgacacgtgt tgtggccgct tcagcccccg gaaagtccac agcgggaaac aggccgcagg
     3361 ggtggcggcg gaagggcaga caactgcgcc atcaggcgca gtttccttac acaccgtcag
     3421 gtgtgcaggg agcgcagatc acggcgcgac agaggcggcg cgaccggaac ggtcgctgca
     3481 agccaggttt ttcagacatc ggaccgtagc ggcagcccga aaccgcgcca gcgggttcgg
     3541 cggagactgg cgggtgcgcg agcagctgcc tgtcggagtc gggcctgacg accggatgcc
     3601 gccaggcagc cgggagccga aggtgtcccc gcctccggaa atcagaacaa cagaaacgac
     3661 ataacagaaa aatgcctcac tccatgacgg gcaggaaggg cagcgccgca ggcgcggccc
     3721 tcctgcattt caggcaggcg taagcctgcc agggttttcc gggggcctgg caggccatac
     3781 ccccgccgga gcggggagta aatcaccgac ggaacacatg accgtcaata aaggtgaagg
     3841 agtccaggaa taaatcccgc gcatacgcct catagtcaaa atagagcgcc accgtctccg
     3901 gcacgtcagc cagcaggccg gtatcactga cgaactcaac cgcaaaagcc tcctcactgt
     3961 cagcctcgcc ccaccaggca tcgcggaagc tgtcaaaatc cgtctcaccg gtatcatcca
     4021 cccagagacg ataagcctct tcgcagcctt catcccgtgc ctggcggaag ccttcaaccc
     4081 aggcccagtt gatatgacat tcagaggcca tattccccgg gaatccctca taatcctgaa
     4141 acatcagttc aggatcggct tcatcctggt gaagagcacg gcaggcggcg aaaaagtcgc
     4201 gctcatcatc aaacgtggtc aggtcaaacc agcgtccggc gatgcttcca cagttgtatt
     4261 tgtgccaggt tccaacgtat acagcaggtg caacaacaga cattcccgaa tcctccatag
     4321 tcacatgttc tgcggcaccg ggcggtgccg tgcctccata aaagccgggc gataaggcgg
     4381 ggatgcacag ggcgcaaggg aagccgcagc ctgacgcaca cggctggcgg gagttggagg
     4441 gtgagaccgc gaaatctgag cgggtgaacg accggaaaat cacggcagac gggaaggagg
     4501 gaccgggtga ccgccgcgtg cattttaccc ttgcatcccc gccagccggc taagatggag
     4561 gcacggcaca gcccgcacac gacgctgagg ccacataact gaaaacccac aaggccatgc
     4621 cccgcatggc cgggtaaaag cggagccgcc ggcgacttcc ttattccggc gagtgcagag
     4681 gacacgccgc atgacctcca gaccacccct gatatgaaac gatcatgatc ggcgtgtccc
     4741 tgcgaaaaat atacatgcct ggttcaccgg agaatatctg tgatatccac aagttttctg
     4801 tcgcacagcc agtcctgcgc cgcacgcact acgctactgt ccggtttcac cgctttcaga
     4861 gaagatgcca gtcgtttgat cagcatcacc agttcccgtg tctgtatgtc cggcattgtc
     4921 tccggcggag cagagggact ctcatttccg acgcccatcc catgcgcttc cagtgtggca
     4981 cggacaaacc aggcagcatc gaccagttgc tggcgaatcc aggccagatc ggcatttctg
     5041 tcacgggctg ccattcgctc acccgacagc gccttgctga tatggctgcg caccagatta
     5101 tccaggagtt cctgcgcttc ttccggtgaa aagccacctg gtatctctgt gcgcaacagt
     5161 cctttcctgt acagtagctg cagcagatca atctgcgacc gcactgcaac tatcagtaca
     5221 taacggacaa tattcgcact ggcggtggtt tctgtatcat tcagcagttc ctccggatca
     5281 ttaccaccgg tattgcagag caacggcaac agaggtcgca tccacacggg aacctgatat
     5341 ttttcgtctg atttcatcat attgtgttct ccagtttgtt cgggatcatc cactgctcaa
     5401 gtgcacgtac ctgtaccggt gcaccacgac cggcctcacc ggcttcaaaa atcagccgcc
     5461 agagtggttc ctcctgccag aaaaaccggt caaatgatga cagtgacgga aaacgcccgc
     5521 accaccccca gaaaatcgtt tccagatcat gctcctcaac aacaccagac cagcgggaca
     5581 accagcttcc gaccgtggcg ggagtatgaa acgcaaggtc aatctgcgcc tgagcaaaac
     5641 atgtctggta ttccagttcc cgtcgttctg cctcacgttc ccgctggcgg ctgtatttct
     5701 gctctgtcag cgtggttttc tgacgacgcc gctcaccttc acgaaaagga acggacggga
     5761 acagacggtg ccccgcatcg gatggcaggg gaagaacaca gagttcacca aaactttcaa
     5821 tcagtttatc cacggcagcc agccacagga gacggttgcc atgaaattcc tccttcgtca
     5881 gcgcaggagc aaaaaagcgg agatcggtca gcgaaatccg gcgactgcca ttcaggcagc
     5941 gaaagaaagc gctggcataa ggccatgctg aaagtgtgcg tccctgacga cgacgttgtt
     6001 cggtgacagc cacagcctgc atggcggcct gttgttctgc gctgaggggg atcagtgccc
     6061 gtgagcgtgc gctcatgcct gacctccttc tgccatcagg atatggatga tgtcgttaac
     6121 actgtacccc tgcgcatcaa gtgtcgcggc aagcgccagc gtgtgactga tagcgacagg
     6181 cgtgaagata tgttgcacct gaacgacggc gaaaggctgt ccaccacaat tcaccagaac
     6241 cagcatccag agctgagaga cgtctcccgg cgaacataat gccaggtgga aacgttcgtg
     6301 ggctggcgtg aaacgaacct ggacggggaa aaagcccccg aactcactgc catactcgcc
     6361 attcatcatc cagttagccg gtgcgtccac ttcacgcatc acctcgtgag tgagattacg
     6421 gcgcatatcg cttccggcag cccggatatc ttcatattcc tgtgcgttca tggactgcag
     6481 ggcattcagg gtcagttctg ttttcatcac ggtatctccc ttgctgccgc cccgtctccg
     6541 gcggggcggt gtggttgttc atcaggcggc atcagccagg ttagtggtgt gttcagtggc
     6601 atccatctgt ggacgtggtg tacacatcca gccaggcacc cagcggttgt ctttcatatg
     6661 gaactctgca tgttcagccg catcgccttt cttcattttc tccgcgtccc gcgcagcacc
     6721 ggacagtccg gcatcattca gggctgcgat aatctgcggc tttttcaggt gtccgaagaa
     6781 gtttgctttt gtcggctgcc accagtcgcg catgtgaaaa ccgatggcgc tctccagcgt
     6841 gtccagcggg ctgcgtgacg tgtgaccaca ctcgcgggtc tgaacgccgt ggatgctgca
     6901 cgcggtgcag aaactgagca gggataacag cacctcctga ctgagtgaaa ggaacgtcgt
     6961 catgtcccgt gaccatccct ccggtaacag ggccgcaaga cgggcttttt ctgccatcat
     7021 cgccatgaac gcggcacctt ccttccctga cggcgcatcg ctggtcagcg aataatgttt
     7081 acattccagg ctgatttgtg ctggtttact gtacgctccg ctgccaaaca cattcaggca
     7141 gagcgtccat gccagcagtg ccagagattt gtccggttgc tgcatcagtg ccgcctggac
     7201 tgccagcgtg cgttcggaag acattttcgt cagcagtggc agactgattt cctccactga
     7261 cgctttctcc tgcacctgtt ccgtgcggtc agcgtcggca gccgcgtcat cctcgctgcg
     7321 caactgcaca ccacgctgga cacacacgtt gccataacgc caggacacca ccacaccgct
     7381 tccggcacgc atctccggcg tccacgccct gaccttcgcc atgcagcgca tcagcttcat
     7441 ttctgcttcc agcag
//
LOCUS       DAZUCC010000222         7452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZUCC010000222 DAZUCC010000000
VERSION     DAZUCC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.223"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..434
                     /gene="hdeA"
                     /locus_tag="ACHY09_004299"
     CDS             102..434
                     /gene="hdeA"
                     /locus_tag="ACHY09_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFS3887183.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            550..876
                     /gene="hdeB"
                     /locus_tag="ACHY09_004300"
     CDS             550..876
                     /gene="hdeB"
                     /locus_tag="ACHY09_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFS3887184.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            940..1587
                     /locus_tag="ACHY09_004301"
     CDS             940..1587
                     /locus_tag="ACHY09_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS3887185.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1639..2409)
                     /locus_tag="ACHY09_004302"
     CDS             complement(1639..2409)
                     /locus_tag="ACHY09_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFS3887186.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(2406..3398)
                     /gene="shuU"
                     /locus_tag="ACHY09_004303"
                     /gene_synonym="chuU"
     CDS             complement(2406..3398)
                     /gene="shuU"
                     /locus_tag="ACHY09_004303"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFS3887187.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(3447..4070)
                     /gene="chuY"
                     /locus_tag="ACHY09_004304"
     CDS             complement(3447..4070)
                     /gene="chuY"
                     /locus_tag="ACHY09_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFS3887188.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGITRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFINRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(4070..4564)
                     /gene="hutX"
                     /locus_tag="ACHY09_004305"
     CDS             complement(4070..4564)
                     /gene="hutX"
                     /locus_tag="ACHY09_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFS3887189.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(4577..5914)
                     /gene="hutW"
                     /locus_tag="ACHY09_004306"
     CDS             complement(4577..5914)
                     /gene="hutW"
                     /locus_tag="ACHY09_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFS3887190.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLTQT
                     WQEMLNQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(5934..6848)
                     /gene="shuT"
                     /locus_tag="ACHY09_004307"
     CDS             complement(5934..6848)
                     /gene="shuT"
                     /locus_tag="ACHY09_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFS3887191.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTATERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
BASE COUNT         1924 a         1788 c         1858 g         1882 t
ORIGIN      
        1 atattttcca tcaacatgac atatacagaa aaccaggtta taacctcagt gtcgaaattg
       61 attcgtgacg gctctttcac tttatagttg aggatattac gatgaaaaaa gtattaggcg
      121 ttattcttgg tggtctgctt cttctgccag tagtgagcaa tgcagcggat gcacaaaaag
      181 cagctgataa caaaaaaccg gtcaactcct ggacctgtga agatttcctg gctgtggacg
      241 aatccttcca gccaactgca gttggttttg ctgaagcact gaacaacaaa gataaaccag
      301 aagatgcggt tttagatgtt cagggtattg caaccgtaac cccagctatc gttcaggctt
      361 gtactcagga taaaaaagcc agctttaaag ataaagttaa aggcgaatgg gacaaaatta
      421 agaaagatat gtaattccgg gaatgcgtta catcgtattt ccttgcatat tgaacaggcc
      481 ggaatatctt ctttaaaagc agctattcct cctgtttgta tataatctct atattgaatg
      541 ggttacaaaa tgaacatttc atctctccgt aaagcgttta tttttatggg cgctgtagcg
      601 gctttgtcac tggtgaacgc acaatctgcg ctggcagcca atgaatccgc taaagatatg
      661 acctgccagg aatttattga tctgaatcca aaagcaatga caccagttgc gtggtggatg
      721 ctgcatgaag aaacagtata taaaggtggc gataccgtta ctttaaatga aaccgatctc
      781 actcaaattc ctaaagtgat cgaatactgc aagaaaaacc cgcagaaaaa tttgtatacc
      841 ttcaaaaatc aagcatctaa tgacttgccg aattaatgag gtacaagtaa aaaggagtag
      901 caagttgagc catcttgctg ctcctttttg catttttata tgacagcaga atttattata
      961 cgacttatac ttgcggtaat tgcctgtggc gctattggca tggaaaggca aatgcgcggc
     1021 aaaggagcag ggttacgcac acatgtatta attggcatgg gaagcgccct gtttatgatt
     1081 gtttcgaaat atggttttgc tgacgtgctg tctttaggtc acgtcggact ggaccccagc
     1141 cgtattgctg ctcaggttgt gacgggcgtc gggtttatcg gtgcaggtaa cattctcgtt
     1201 cgtaaccaaa atattgtagg tctgacgacg gcagcggata tctgggtgac cgccgccatt
     1261 ggtatggtta ttggcagcgg tatgtacgaa ctgggtattt atggttcagt gatgaccttg
     1321 ctggtgctgg aagtcttcca tcaattaacc ttccgcctga tgaacaaaaa ttaccattta
     1381 cagctgacat tagtgaacgg taatacggta tcaatgctcg actggtttaa gcagcaaaaa
     1441 ataaaaacgg atttagtttc gttgcaggaa aatgaagatc atgaagtggt cgccattgat
     1501 atccagcttc atgccacgac gtcgatagaa gatctgttgc gattattaaa aggtatggca
     1561 ggggtgaaag gcgtttcaat tagctaatct gtatagttat ttattcgcag gagtaccagt
     1621 gcggtactcc tgttggtttt aatgatcgag gacgatcagg ggtgaatggt tggcaggatc
     1681 tttaagtacg gtaatatccg cgccgtaaag catggtcagc gcctgctgtg tcaatacgtc
     1741 ctgtggttta ccatttgcga taacctttcc tttttgcatt aatacaatac ggtctgcata
     1801 acgcgcggca agatttagat cgtgcaatac gcagcaaaca ttgaactgcc gttcgtgaac
     1861 taactggcgt aataaacgga acaagtgctg ctgatggtga atatccagcg ccgaagttgg
     1921 ttcatcaaga aataaccatt tcggtgaggg agttggttcc cagagttgca ccagcaggcg
     1981 cgctaattgt acgcgctgtt gttcaccacc ggataattgc cgatagtcgc ggtttgctaa
     2041 cgcctgacag tcacataacg ccattatttg tgctgtttca ttatcctgat taccagtgcg
     2101 atgcggatgc cgccccatct ggataacttc ttgtacgctg aatggaaacg ccatgtggct
     2161 attttgacgc atcacggccc ggtgtttcgc cagttcagta atcgaccatt catttaatgg
     2221 cttattaaac aaccgacatt cgccgctatc aggttgaaga tagccggtta actggcgaag
     2281 cagcgtagat tttcccgcac cattagggcc gagaatggca acaatttccc caccgggaaa
     2341 agtcagcgag acattatcag tcagacgccg cccctgaagg ctataaacca gattctgggc
     2401 gctgatcatg tgatcctccg gttgcgcaga atcaaccaca taaaataagg accaccaagc
     2461 aggctggtta ataatcccac gggcatttct gctggctgta ccagcgtgcg ggcaagcgtg
     2521 tctgccatca gcaataaaca ggctccggcg agggcggagc aagggattag ccagcggtga
     2581 tttgctccgg tagtcatgcg aatcagatgc gggatcacca ggccaataaa gccgataatg
     2641 ccgcttaccg atacggcggc accaacgagc agtgagctca ctaacagtaa ttgctggcgt
     2701 ttacgcttaa cgttcacgcc gaggtaatgg gcttcttcat caccgagctg cagtaaattc
     2761 agcgttccgg cgagacaagt tgttgcgata atggccggta ggatgaatga actggcaacc
     2821 aataacgtcg gccattgcgc ctgtccaaga ttgcccatca tccacaatgt gagttgtcgt
     2881 agctgctgct catcgccgat atagctcagg atgccgatcg ctgcgccgca cagggcattg
     2941 atggcgatac cggcgagtaa taattgcatc atgctgccat cgcgatggcg ctgcgtgatg
     3001 agaaaaatga tggtgcagac cactaaactt ccggcaatgg caaacaccat ttgctcatag
     3061 agcaccagca ccactgggaa ggagaagggc agcacaatag caacgccaac cattaatgca
     3121 gaaccgctac tgacgccaag taatccaggg tcagccattg ggttgcgaaa aagcccctgc
     3181 ataattaccc cggcggttgc caacgctgca cctaccagca ccgccagcaa aacccgtggt
     3241 agacgaatat tgagccagat atcgcggtat tcctcatcaa aaagggcatc aaaactgatc
     3301 tttaacgctc cctgactggc accaaacagc accagagcta gcaataatag tgatagcccc
     3361 ataaagaccc tggcggaaga gagtgaatcc ttgagcacgc aaaactccgt tacgacatta
     3421 cggcgcggac atgccgcgcc gggggatcac gccggtttca ggtccggttc aatcagactg
     3481 tagacctgct ggttaagtgc tggcgcattt gccagttcat ggatatgtgc ggcgacatca
     3541 gcacggttaa taaaaccatg acactcctga ttttgtatcc gttgtgcttt gcccgttgcc
     3601 gcaccatcaa gcagaccgcc agggcggaga atggcataat caagctggct ggtttgcaac
     3661 cagctttccg ccagcgtttt ttcccgtacc gcctgaccaa acgcagcctt tgcccgctct
     3721 gataaaaacg gccagctatc gccgcatccc agtgacgtca ccagaatcat gcgtgtgatg
     3781 cctgctttct cggcctcgtc aatcactgtt ctgtgcgcca gataatcctg agctccgccc
     3841 attgttgaga tgataagcgc atccgggcca gctgcgcggc aagcagccgc gactacgctg
     3901 gcatcacagg cgtcgcctgt aaaaacctgt acgccttgtt gcgccagttt cgtggcggca
     3961 tcggcatgac gaatgacagc aacgaccgga cgttgttccg ccagcgccag ttcaagcgtt
     4021 ctggcaccca caccttttcc gcctgcgcca aagagtagcc acggtgtcat caggcgtgtt
     4081 cctttaacga tgctgccaga gcatggaagg cactgacttg ttcgctcagg agctgacgat
     4141 gatcatccct accgaggaag attttcagca ttgcgctgcc ttccttattg aaaaagagaa
     4201 tagaggcggt atccatgccc ataaatttac gttcgatcaa ggcaatatgc gtgcagtttt
     4261 ctgctttgat atgcccggac atgccgtgct ttccgcgtag gttgaaatag ccgtggcgat
     4321 ggaaacccga aggcagttcg ccgctaaatt cgagtattac atcggtagta tgtactaacg
     4381 tggtgacgtt gccccattcg cagacggtat cccagacagt atcgaattta tcacctgaca
     4441 caacagtcgg agagggcaga tttttcacta cctccagcag cgtggtgttg tattgttcgg
     4501 ctatcgcctc cagcgtaccg tctggctccg ttttcaaaaa ttcctgtaac gagacatggc
     4561 tcataaatca ctcctgttat ggtttttcaa gcgcaattgc tggcgcatta agtacctgaa
     4621 tcagttcatt aagagactgc aaaatgttgc ttgcccagaa acgaccttca ttggtcaacc
     4681 gcagacacgt tgacgcatcg cggcttaagc ctttttggtg ccactgggcc agtaacggcg
     4741 cgagtttttc ggcatgtggc gtaagttcgt ccagcggtac acgcgccgtt tcaatgcccg
     4801 actgcaaggt atgacgccac tgcgcgttac gttcggcgtt acgcatgatc atcatcagcg
     4861 gttttttacc tgctgcgacg gattcatgcc aggtctgaag attgcgttcg ttcatccagg
     4921 agtaaccatt aatcgaaccg ccggctccgg aaccaaaggc cagacaatcg gcaccttgtt
     4981 taatcagcag gttatagaga ttgcgttcgc gtgtggtacg gccccagtgg ctgttactga
     5041 tgcagcgcca accggcatcg tccataaaat cacacccttg caggtaaaga tcgcgacgtt
     5101 ctgccggaga gggcacggta gtacgcccat tttccacggc tttgcccagc ggtgtattgg
     5161 gcaggacatt gagcgcatag agatcgacgc cgtcgagacc gatatcgcgg gcaatagcca
     5221 gatcttctcc ccaggtttgc gcatcctgac ccggcagacc aaacagcagg tcacagacca
     5281 ccgcggcacg gtcgcgtttg accaggcttt ccataaacgc aatagcggtt gggccatccg
     5341 aggtgcgggc cattttcttg cggattttgc tgttaaacga ctgaatacca attgagaagc
     5401 ggtttgctcc agcatcaagg caagcatcga ttcgctcagc gtcaaaattc agtacccggc
     5461 cttcaatagt gatttcacaa tcaggggcca gtggcagctt ttctcgtaat gtggtgatga
     5521 ttctggccaa atcgtgtgct gaaagcgcgg aaggcgtacc gccaccgaaa tagaccgcgt
     5581 ggatgggggc agactgatgc aatacgctgt ctgcttccat ttctatttca cgaatcaggg
     5641 cgtcggtata atgagcacac gcatcgtcat taaaacggtt ctgataaaaa ccacaaaacg
     5701 tgcagtgtgt cgcacaaaaa ggaatgtgta gataaaccag tcgcttacgt ggtgataccg
     5761 tttgattgag catctcttgc caggtctgtg tcagttgctc tttggcaact ggaatggccc
     5821 cacgaaacgg catcatggcg cgcctgtctt taaatggctg atcaccgtct aaagcaaaat
     5881 gtggcgtgag atccagggtg ttatttgtgt tcattcgtct gatttaaatc catttattgc
     5941 ggccaaagct gctggtgtaa agactcaacg acatcagcaa tacgcggacc cattcccaga
     6001 atcaggtttt gatcaacggt gataatgcgc tggtttttcc atgcggcagt gtgggtaatt
     6061 ccggcaatag aacgtagccg attaatatcg ccatcgacca tttgtgaggt gacgacaatc
     6121 acttcagggt tagccgcaat cagcgattcc gcgctgtagc ttttgtactg ctggtgggta
     6181 gcgacgtttt ctgctccggc aagcgaaaga atggcgtccg cgacgctgcc tttaccggca
     6241 acctgtggcg cacttccgcc tgcggagaga ataaacatgg ctttaaccgg ggcttttttg
     6301 gccgccacgt tttgctgtac tcgctccagg cgttggttga tctgtgtcac cagcgcctcg
     6361 ccttgttcag gaacctgtaa cgtttttgcc agttggcgaa tgttggcgta catctgctca
     6421 agggtggctg gtacacgcgg cagagtgacg acgttgactt tttgcgcccg cagttggtcg
     6481 agcacaattt gcggtcctgc atcctgccag gtaattacgc tatccgggcg aagtgacaaa
     6541 atgccttcgc tgctgagttg tttccagtaa ccaatatgcg gcagtttggc ggtttctggt
     6601 ggataagatg tcgtttcatc gacaccaacc acgcgttcgc cagcgcccat cgcgtagatt
     6661 agctccgtca gcgatcctcc tgcgaccacg atacgttcgg tagccgttac gctaaaagcc
     6721 cagctaagag ccagtactgc cgtaaacaat ttttttcgtt ttaccgcggt cttcgcggca
     6781 gaggccacgg ctgaaataca taacgtcagt ggggaaaaaa gaaacggcct ggtgatgatc
     6841 cttggcataa tatgagaact cttatcaaat tgagaatgat catgattatc atttgagtta
     6901 tatatgaaat acaaccgtcg attcaatacg cacggcgtta caagcgtatt tatttttgat
     6961 ttttatcaag atgcttcgtc agctaacgtt cttgttaccg actaaatttc agcgcgggaa
     7021 aaagctgaga acccagagat atcgcggctt gcagagcaaa gagaagagaa aattatcact
     7081 gttaacgaca aagtttagtg atgaggtcat gggggaataa ccataaaaac aaagtggttt
     7141 ccagggcgca ggataagttt cataaccccc tgtttattgt gttaatggcg gttaaaaaaa
     7201 agcttgatca taaaaaattg atcgataata attctcatta tcacgtttgc atttaacaaa
     7261 atacggccat atggataaca aagccatatc atttacaagg ggtaacgcac agcgctcttc
     7321 actcctgatg gggcatatcg gtttgcctga caaagagggc gtgcttttgc gtatttgccg
     7381 agataccagg caatttcatg ggatataaac gcctgtaaac tcacccggcg aatattatcg
     7441 ctctgaattt ta
//
LOCUS       DAZUCC010000223         7393 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZUCC010000223 DAZUCC010000000
VERSION     DAZUCC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.224"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..200
                     /locus_tag="ACHY09_004308"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACHY09_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(289..1425)
                     /gene="yibH"
                     /locus_tag="ACHY09_004309"
     CDS             complement(289..1425)
                     /gene="yibH"
                     /locus_tag="ACHY09_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS3887192.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1428..1790)
                     /gene="yibI"
                     /locus_tag="ACHY09_004310"
     CDS             complement(1428..1790)
                     /gene="yibI"
                     /locus_tag="ACHY09_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFS3887193.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2327..4240
                     /gene="mtlA"
                     /locus_tag="ACHY09_004311"
     CDS             2327..4240
                     /gene="mtlA"
                     /locus_tag="ACHY09_004311"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFS3887194.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4605..5753
                     /gene="mtlD"
                     /locus_tag="ACHY09_004312"
     CDS             4605..5753
                     /gene="mtlD"
                     /locus_tag="ACHY09_004312"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS3887195.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIRK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5753..6340
                     /gene="mtlR"
                     /locus_tag="ACHY09_004313"
     CDS             5753..6340
                     /gene="mtlR"
                     /locus_tag="ACHY09_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFS3887196.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6351..6560)
                     /gene="yibT"
                     /locus_tag="ACHY09_004314"
     CDS             complement(6351..6560)
                     /gene="yibT"
                     /locus_tag="ACHY09_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFS3887197.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            6844..7206
                     /gene="yibL"
                     /locus_tag="ACHY09_004315"
     CDS             6844..7206
                     /gene="yibL"
                     /locus_tag="ACHY09_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFS3887198.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1886 a         1857 c         1852 g         1798 t
ORIGIN      
        1 catccttcag ataataaagc catcatcgca atagatggaa aagcactcct gctctttttt
       61 gattagagcc gtggcagagg aacaactcat atcatcattg cgatctcgac aatgtatagt
      121 ctgatccttc ggcagatcaa gacggataaa aaatccaatg agttcacact catcccgaac
      181 ttcttaacat aatggatatt aacgaaaaat catcacagcc gatgaaataa tatgcaaaaa
      241 taattagtcc ttacaaggag caaagttcca taatctcccc cctccccctc aatgatccaa
      301 ataaagataa tgcatccaac tggtcattct cagcagcact ttccgcatca ccgaaacatg
      361 gctgaaatgg tcggagtaaa ctgccacttg ggcgtagatg ccgtcgggta aggcatcgat
      421 atcatcgtta gggtccagtt caatggttcc cagcacaccg tccgtgccag gcacgaccgt
      481 taatgattgc aataccccct gcgcctgata agaaccgcct ggcacgacag gtaaaatact
      541 agtcagtttg ccgtgaaaca cctgcccagg tagcgcgtta aacaccactt ccgcatcatc
      601 gccgggtttc agacgtaaca gcgagttttg ccgaaattgg gcgacaattt gccgtttttg
      661 ctcggggatg aaaaccatca ccggacgcaa cggcaaggca gctgcgtatg tacctgggcg
      721 gatcagtacc tgagtaacgt agccattgct tggtgcgcga atgacagtct gctcaaggtt
      781 atattttgct tcagtaagtt gcgctcttaa gctcacaatc tgcgattgct cgccgttaac
      841 catactgtcg agctggctct ggatctgcgc ctgctccgcc accgagcctt tcaccagcgc
      901 gtcctgcgcg aggaaatttt gccgcgcatc gtcgatgtca cgttccgaga acggattcac
      961 cgccgcctgg ctgcctttca ggtaacgttg ataattttta aacagacggt cgcgctccgc
     1021 tgaaacctgg gtggtgttgg cctgcgcttc agtgagctgc gcgcgcagcg tctttatatt
     1081 atgcgtcgcc gtcatcaggt cagcctgaag tctgtcaact cgcgcctggt aacgaaccgg
     1141 gtcgagctta aaaagcacct cgcccttttg aataagctga ttatttttgt cggtgacttc
     1201 agtaacaatt cccgtcacct gtggcgtgat ggggatcgct atcactgcct tttgcgcggt
     1261 aaaagtgtaa gggtggttgt agttcatcaa caaaatcaaa ccactcacca gaaacacgcc
     1321 tcccagcgcc gcggtcgcca gcgtccactg attcaccgga atgcggaaga ttttaaagac
     1381 cgcccacgcc agcgccacgt aagttaaaac aatcaataga tccatattta gatctccggg
     1441 aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg ggaaattcgg
     1501 tcagaaagtg cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc
     1561 ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata
     1621 acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatga
     1681 ttgcgctttt tggcaatcag atatgggatg tcatgtatcg cgatgattcc ataaaaaatc
     1741 accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg
     1801 gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca ggctgatatt
     1861 ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg
     1921 atgagttgct tagttacata acgattttat gacgaaggca taacatgctg tagatcacat
     1981 caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagatcca acaaaccacc
     2041 aaaacaaaaa tgtgacatta gtcacattta aatgccattt ttagcgaaaa tcgccgtctt
     2101 gttgcttttt tacacaagca ttttgtgatg agcatcacgt caattacctc tctaccccct
     2161 atatttatgt gacccatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg
     2221 ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct
     2281 cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat
     2341 taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg
     2401 cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa
     2461 cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg
     2521 ttataccggt ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac
     2581 catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc
     2641 gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg
     2701 ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct
     2761 ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt
     2821 taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa
     2881 aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gcattcagca
     2941 gtcccatgaa ttgggtaaat ccatcttctt cctgattgaa gctaacccag gtccaggtat
     3001 gggcgtgctg ctggcgtaca tgttctttgg tcgtggcagt gctaaacaat ctgcgggcgg
     3061 tgcggcaatc atccacttcc tgggtggtat ccacgaaatt tacttcccgt atgtgttgat
     3121 gaatccgcgt ctgatcctgg ccgttatcct cggcggtatg actggcgtgt tcacgctgac
     3181 tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc
     3241 gatgacacca aaaggtgctt atttcgctaa catcgcaggt gtgtgtgcgg cgatggctgt
     3301 ctccttcgtt gtctctgcca ttctgctgaa aaccagcaaa gtgaaagaag aagatgatat
     3361 tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggtg cgtctccgct
     3421 gtctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga
     3481 cgccggtatg ggttccagtg cgatgggcgc aggtgtgctg cgtaagaaaa ttcaggatgc
     3541 aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga
     3601 cctggtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcaca
     3661 gcatatttcg ctgaccaact tcctcgacag cggcttgtac accagcctga ccgaacgtct
     3721 ggttgctgcc cagcgccata ctgaaaacga agtaaaagtg aaagacagtc tgaaagacag
     3781 ctttgacgat tccagtgcta acctgttcaa gctaggcgcg gagaacatct tcctcggtcg
     3841 caaagcggcc accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg
     3901 ttacgttgag ccagaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta
     3961 tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcaaaagatc gcgtactgaa
     4021 aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga
     4081 cattgcccgt ctggtgattg gtattgctgc ccgtaacaac gagcacattc aggttatcac
     4141 cagcctgacc aatgcactgg atgatgaatc cgtcatcgag cgtctggcac acaccaccag
     4201 cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tacaatctca ccctctccac
     4261 atggagaagg tggggcttat tgcctgatgc gctacgctta tcaggcctac aaaacaaagc
     4321 acagtttgtt gaatttgcac gttcttgtag gccggataag gcgtttacgc cgcatccggc
     4381 gctgcccctc tccacatgga gaagatgggc taattgcctg atgcgctacg cttatcaggc
     4441 ctacaaaaac aaagcacaat ttgttgaatt tgcacgttct tgtaggccgg ataaggcgtt
     4501 tacgccgcat ccggcgcggc ccctctccac atggagaggg tttgggtgag ggaaaagcct
     4561 caccccagcc ctctcgggta aaaacattga tgaaggttaa tactatgaaa gcattacatt
     4621 ttggcgcagg taatatcggt cgtggcttta tcggtaaact gctggcagac gcgggtatcc
     4681 aactgacgtt tgccgatgtc aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct
     4741 atcaggttca tgtggtcggt gaaaccgagc aggtagatac cgtttccggc gtcaatgccg
     4801 tcagcagcat tggtgatgat gtcgttgatc tgattgctca ggttgattta gtcactaccg
     4861 ccgttggccc ggttgtgctg gaacgtattg ctccggcaat cgccaaaggg ctggtgaaac
     4921 gtaaagaaca aggtaatgaa tccccgctga acatcatcgc ttgtgaaaac atggtccgcg
     4981 gcaccacaca gctgaaaggc catgtgatga acgccctgcc agaagatgcc aaagcgtggg
     5041 tagaagaaca cgttggcttt gtcgattccg ccgttgaccg catcgtgccg ccttcggctt
     5101 ctgcaaccaa cgatccgctg gaagtgacgg tagaaacatt cagcgaatgg attgtcgata
     5161 aaacgcagtt caaaggcgcg ctgccaaaca tccctggcat ggaattaacc gacaatctga
     5221 tggcatttgt cgaacgtaaa ctcttcaccc tgaatacagg tcatgctata accgcgtacc
     5281 tcggaaaact ggccggtcat cagaccattc gtgatgcgat tctcgacgag aaaatccgcg
     5341 cggtggtaaa aggtgcgatg gaagaaagtg gtgcagtatt gatcaagcgc tacggctttg
     5401 acgcagacaa acatgcggcg tacatccgga aaatcctcgg tcgtttcgag aacccgtacc
     5461 tgaaagatga tgtagagcgc gtaggccgtc agccgctgcg taaactgagt gctggcgacc
     5521 gtctaatcaa gccactgctc ggaacgctgg aatatggtct gccacataaa aacctgattg
     5581 aaggtattgc cgctgcaatg cacttccgca gtgaagatga tccgcaggct caggaactgg
     5641 cagcactgat cgctgacaaa ggtccgcagg cggcgctggc acagatttcc ggtcttgatg
     5701 ccaacagcga ggttgtatcc gaggcggtaa ccgcttataa agcaatgcaa taatggtgga
     5761 ccaggcgcag gacaccctgc gcccgaataa cagattgtca gatatgcagg caacaatgga
     5821 acaaacccag gcctttgaaa accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg
     5881 aagctttctg atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca
     5941 ggtattccgc aaagacgatt acgcggtgaa gtatgctgta gaaccgttac tcgacggcga
     6001 cggtccgctg ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa
     6061 ccgccaggaa tacgaagatg cggaactgct gatggcattg cgtgaagagc taaatcacga
     6121 cggcaacgag tacgcgttta ccgacgacga aatccttgga ccctttggtg aactgcattg
     6181 cgtggcggcg ttaccaccgc cgccacagtt tgaacctgca gactccagtt tgtatgcaat
     6241 gcaaattcag cgctatcaac aggctgtgcg atcaacaatg gtcctttcac tgacagagct
     6301 gatttccaaa atcagcttaa aaaaagcctt tcaaaagtaa gtactttcgc ttacttctcc
     6361 tctacctgtt tcggacgata aagctgacga taatactcca gacgttgtag atataacggc
     6421 acgctttcat caggtattcc ggacggaatc gcgttacggg gaggtagttt tttcagatac
     6481 tcccggaacg cctggctcga tgccatgaaa tctacggctt tatcgataag aagcggaacg
     6541 ttttcaccta atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc
     6601 cgccaatttt agtttagatc ccggtataaa tgacattaag gaaaacgtgc tcaatcctca
     6661 ataatcacct gcttctgttg actatctttt ttctttaaat ttcttaatta atcaataaat
     6721 tgcgtagttt ttaagcatgt taatgtttac gttatagaac tattgcaatg tcatcagttg
     6781 tgaacaaagc acctggtcgc gcatactagg ggctataaat ttatctttat cagaagccat
     6841 cacatgaaag aagtcgaaaa aaacgaaatc aaacgtctca gcgatcgtct ggacgccatc
     6901 cgccaccagc aggccgatct gtcgctggtt gaagccgcag acaaatatgc cgaactggaa
     6961 aaagagaaag ccactctgga agcagaaatt gctcgcctgc gcgaagttca cagccagaaa
     7021 ctcagtaagg aagcacaaaa gctgatgaag atgccattcc agcgcgcgat taccaaaaaa
     7081 gagcaggctg atatgggcaa gctgaagaaa agtgttcgcg gactggtcgt tgtgcaccca
     7141 atgaccgcgc tgggccgcga aatgggcctg caagagatga cagggttctc aaagactgcg
     7201 ttttaagttc ctttctcgtc gctgtaatca cagcctctca ccatttactc gctcctgctg
     7261 gcacatgcag gagcctgttt cagagatcga atatttataa cgttcttaac attatatatt
     7321 caatctcctc tattcttttt agcgcacatg ccaggctaaa ttctgaaaat caggtacaag
     7381 ttaaagaacg atg
//
LOCUS       DAZUCC010000224         7287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZUCC010000224 DAZUCC010000000
VERSION     DAZUCC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.225"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            96..1463
                     /gene="ydfJ"
                     /locus_tag="ACHY09_004316"
     CDS             96..1463
                     /gene="ydfJ"
                     /locus_tag="ACHY09_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFS3887199.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVTAAQ
                     PSRSL"
     gene            1553..3013
                     /gene="ydfI"
                     /locus_tag="ACHY09_004317"
     CDS             1553..3013
                     /gene="ydfI"
                     /locus_tag="ACHY09_004317"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS3887200.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAVMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3049..3252)
                     /gene="ydfZ"
                     /locus_tag="ACHY09_004318"
     CDS             complement(3049..3252)
                     /gene="ydfZ"
                     /locus_tag="ACHY09_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFS3887201.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3429..4115)
                     /gene="rspR"
                     /locus_tag="ACHY09_004319"
                     /gene_synonym="ydfH"
     CDS             complement(3429..4115)
                     /gene="rspR"
                     /locus_tag="ACHY09_004319"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFS3887202.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTHHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4204..4950)
                     /gene="ydfG"
                     /locus_tag="ACHY09_004320"
     CDS             complement(4204..4950)
                     /gene="ydfG"
                     /locus_tag="ACHY09_004320"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFS3887203.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDSKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5087..7132
                     /gene="dcp"
                     /locus_tag="ACHY09_004321"
     CDS             5087..7132
                     /gene="dcp"
                     /locus_tag="ACHY09_004321"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFS3887204.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQAPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELVSIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KSAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            complement(7177..>7287)
                     /locus_tag="ACHY09_004322"
     CDS             complement(7177..>7287)
                     /locus_tag="ACHY09_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887205.1"
                     /translation="YYNGRVYKKSNFAGDCETAFALAVKFALTQRLQLLL"
BASE COUNT         1813 a         1694 c         1867 g         1913 t
ORIGIN      
        1 aacattgctg caaagtctta cttacttttt ctgaataata ccaataacgg cgagtaagta
       61 gtacgcatct tacctctttt ttagagataa ccattatgac aatagaaaaa cacgaaagaa
      121 gcactaagga tttggtgaaa gcagcagtat cgggatggct gggcactgcg cttgaattta
      181 tggatttcca gttatattcg ctcggtgcag cgttagtgtt tcatgaaata ttttttcctg
      241 aatcatcaac ggcaatggcg ttaattctgg caatgggaac ctacggtgca ggttatgtgg
      301 cgcgtattgt cggagcattt attttcggca aaatgggcga cagaataggg cgtaaaaaag
      361 tactctttat taccatcacc atgatgggga tctgtaccac cttaattggt gtgttaccga
      421 cctatgcaca gattggtgtt tttgcaccca tcttgctggt gacgctgcgt attattcagg
      481 gattgggtgc aggtgcggaa atttccggtg ccggtacgat gctggcggaa tatgcgccaa
      541 aaggtaagcg cggaattatc tcctcatttg tggctatggg aactaactgc ggaaccttaa
      601 gcgcaacggc aatctgggcc tttatgttct tcattctcag taaagaggaa ctgttggcgt
      661 ggggatggcg tataccgttc ctggcgagcg ttgtcgtgat ggtctttgct atctggttgc
      721 gtatgaatct gaaagaaagc ccggtctttg agaaggttaa cgacagcaac caaccgacag
      781 caaaacctgc acctgctggt agcatgttcc agagcaaatc cttctggctg gcaacagggc
      841 tgcgttttgg tcaggctggt aactccggtt taattcagac tttccttgca ggctatttag
      901 tacagacgtt attgtttaac aaagcaattc caacagatgc attgatgatc agttcgattc
      961 tcggctttat gaccattccg ttccttggtt ggttatccga taaaattggt cgccggatcc
     1021 cgtatattat tatgaatacc tccgcgattg tgctggcatg gccaatgctt tctatcatcg
     1081 tagataaaag ctatgccccg agcaccatta tggtggcact gattgtgatt cataactgtg
     1141 cggtgctggg attatttgct ctggaaaaca ttaccatggc agaaatgttc ggctgtaaaa
     1201 accgctttac ccggatggcc atttctaaag aaattggtgg tcttatcgct tccggttttg
     1261 gtcctatcct ggcgggtatt ttctgcacca tgacggaatc ctggtatccg atcgccatta
     1321 tgatcatggc atattcagtg attggtttaa tctctgcgct gaaaatgcca gaagtgaaag
     1381 accgtgattt aagtgcactg gaagatgccg cggaagatca accgcgtgtt gtaacagctg
     1441 cgcaaccttc aagaagtctg taaatcctcc atcccttctc ttaccggaga ggggattttt
     1501 tattcatata acaaaacatg cagcttgcca tatttaaatt taaggaattg ttatgggaaa
     1561 taatttgtta tcagcaaaag cgacgctccc tgtttatgat cgtaataacc tggccccaag
     1621 aattgttcat ttaggctttg gtgcatttca ccgtgcgcat cagggtgtgt atgccgatat
     1681 tcttgctacg gaacatttca gtgactgggg atattatgag gtcaatttaa tcggcggcga
     1741 acagcaaatt gccgatttac aacagcaaga taatctttat accgttgcgg aaatgtcggc
     1801 cgatgcgtgg acggctcgcg tcgttggcgt cgttaaaaaa gccttgcacg tacagattga
     1861 tggcttagaa accgtgttgg ctgtgatgtg tgaaccgcaa atcgcgattg tctctctgac
     1921 aatcaccgaa aaagggtatt tccactctcc ggcgaccgga cagttaatgc tcgatcaccc
     1981 gatggtcgtt gccgacgtac aaaatcccca ccagccgaaa actgcaacag gggtgattgt
     2041 cgaggcgctg gctcgccgta aagcggcagg acttcccgca tttaccgtca tgtcatgtga
     2101 caacatgcca gaaaacggtc atgttatgcg tgacgttgtc acttcctacg cgcaagctgt
     2161 tgatgtaaaa ctggcacaat ggatcgaaga aaacgtgact ttcccatcaa caatggtgga
     2221 ccgtattgtg cccgcagtga cggaggatac gctggcgaaa atcgaacaac ttaccggtgt
     2281 gcgcgatcct gcgggcgttg cctgtgaacc tttccgccag tgggtaatag aagataactt
     2341 tgttgccgga cgtccggaat gggaaaaagc gggagccgaa ctggttagtg atgtgctgcc
     2401 ttatgaagag atgaagttgc gcatgctcaa cggcagtcat tcattcctgg cgtatctggg
     2461 gtatcttgcc ggatatcagc acattaatga ctgcatggaa gatgaacatt atcgtcatgc
     2521 agcgtatgcc ttgatgttgc aggaacaagc gccgacgttg aaagtgcagg gcgttgattt
     2581 gcaagattac gctaaccgat taattgaacg ctatagcaac ccggcgctac gtcatcgaac
     2641 ctggcagatt gcgatggatg gtagccagaa attgccacag cggatgttgg attctgttcg
     2701 ctggcatctg gcgcatgaca gcaagttcga tctgctggcg ctgggcgtcg caggttggat
     2761 gcgttatgtc ggtggtgttg atgaacaggg aaatccgata gaaatcagtg acccactgtt
     2821 acctgttatt cagaaggctg tacaaagtag tgccgaaggg aaagcgcgcg tccagtcatt
     2881 gctggcgatt aaggcgatct ttggtgatga tttgccagac aatagcttgt ttactgcaaa
     2941 agtgacggaa gcgtacttgt ctttattagc gcatggtgcg aaagcgaccg tggcgaaata
     3001 ttccgtgaag taaaaaaagc aatgccgcca tcggcggcat tcacacgctt agttcatgcc
     3061 gagacgaatc aggtccagag gtgccagttt ctcttcacaa ccttcaacaa ctacggtttt
     3121 tccgcggcgg atttgctcag cggtcagacc ttcagtatca atcgacagga tcttgccagt
     3181 gtgaccggtg ccgctaacca taacacggct gccagtggtg attgcgttac ggttacgatc
     3241 gtaggtcgtc ataggtgttt tctcctttct gatttacagt cgaagcccac gtaacgggcg
     3301 cactataaat actcctgtcg agtgaaatat tttttgtttt tgatcaagct cacatctttt
     3361 ttgatgcatg catgagccat atgacggagg gaaaaactct caccccggat gggatgagag
     3421 gaaatgaatt actcttcgct aaaccagtcg ctgttttcct gacggatctg gcgcacggat
     3481 tcgctgattt cctgcaaatg gtgcgtcatt gcacgttcta ccgcatcgcc atcacgtttt
     3541 tgcagggcag agaaaatctc aagatgctgg cgtaacagca tttctggtgg agaaacgtgg
     3601 tcgaaactca tatagcgcac gcgatcaacg gtcgctttca ggttctcaat ggtatcccac
     3661 gccagttgac agtcggcaat ctgcgtcagg agttgatgga agttgtcatc aagttcaaaa
     3721 aaatcatcca gttgcttgcg ctctatggca atgcgttgct ggtgaagatt ttgttccagt
     3781 tggtagcact ggctttcggt aatcatgctc gccgcccgac gcgccaccgc gcactcaatg
     3841 gcctgacgga taaaactgcc gttgcgcacc tgggccatgg aaattttgtt gacgtagctg
     3901 ccacgctgcg gacgaatttg aatcaggccg ttttccgcca gtttaataaa ggcttcacga
     3961 accggctggc gtgacacatt gaaacgaaca gaaacttctt tttccgacaa cggtgtgcct
     4021 ggtgcaatca ggcaatggac aatgtcgcga cgaagaatac gataaatctg ctgattgaca
     4081 ggctgtgtgg gattaagttg cgtttcgacg gtcattcgtt cttgcttgtg agtgagttaa
     4141 ctgcatgagt ctaccacttt tttgtgacaa gcaataaccc ggcagttacg ccgggtataa
     4201 aaattactga cggtggacat tcagtccggc atagctttgg gtaaccggca tcatttccag
     4261 ggtattgata ttgacgtgag caggcagcgt tgacacccac cagacggctt cgctgacatc
     4321 ttccggcgtc aatgcaacgg tattttgata ggttttttcc gctttactgt catcgccttt
     4381 aaagcggaca ttggaaaact cggtgccacc caccagaccc ggttcgatgt cggtgacgcg
     4441 caccgccgta ccatgcaggt ccgtacgcag attcaggcta aactgacgaa caaacgcttt
     4501 cgtcgcaccg taaacgttac caccggcata cggccagctg cctgccgttg agccaatgtt
     4561 aataatatga ccatgattac gttcaaccat acccggtaag acggcgcgcg tcatatatac
     4621 caggcctttg ttgttggtat caatcatcgt ttcccagtct tcaacgctgg ctttatgcgc
     4681 aggctccatg cccaacgcca ggccggcatt atttaccagg atatcaatat tgcaccactc
     4741 ggcaggaagc gatgccagca tctcttcaat agcggcgcgg ttgcgaacgt ccagttgggc
     4801 gatatacaga ttatctccca gttcgtcttt taactcctgc aaccgctcct ggcgacggcc
     4861 agtggcgata actttatgcc cttgttgaat aaaacgacga gtaatgcatt caccaaaacc
     4921 tgccgttgct ccagttacta aaacgatcat ctcactgttc ctcaacgctt ttgtgtgtca
     4981 ataacatagc acgtgatgct taagagcggg taatactggt ttgcaagatt gcactccgtt
     5041 atcagtaagc ctacgctatg gaacacctcg cgttcaggag aagaaaatga caacaatgaa
     5101 tcctttcctt gtgcaaagca cactgccgta tctggctccc cattttgatc aaattgccaa
     5161 tcatcactat cgcccggcat tcgatgaggg aatgcagcaa aagcgggcag aaattgctgc
     5221 catcgcgctt aacccgcaag cgcctgattt caacaatact attctggcac tggaacaaag
     5281 cggagaatta cttacccgcg ttaccagcgt cttttttgcg atgactgcgg cgcatactaa
     5341 tgatgaatta cagcgtcttg atgaacagtt ttccgctgaa ctggcggaac tggctaatga
     5401 tatctatctg aacggtgaat tattcgcgcg ggtagatgct gtctggcaac gccgtgaatc
     5461 cctgggactt gatagtgaat ccatccgcct ggtggaggtc attcatcaac gttttgtcct
     5521 tgccggagcc aaacttgcac aagctgataa agcaaaatta aaagtactga atacagaagc
     5581 tgcgaccctg accagccagt ttaaccagcg gttactggca gcaaataaat ccggcggtct
     5641 ggttgtgaac gatttcgcgc agctggcagg aatgagtgag caagagattg cgctggcggc
     5701 agaggcggct cgcgagaaag gtctggataa caaatggctg attccgttgc tgaataccac
     5761 ccagcaacca gcgcttgctg aactgcgcga tcgcgcgacg cgtgaaaaac tgttcactgc
     5821 gggctggacg cgagcggaaa aaaatgatgc caatgatacc cgcgctatca ttcaacgtct
     5881 ggtggagatc cgcgcgcagc aggcgaaact gcttggtttc cctcattatg ccgcatggaa
     5941 aatcgccgat cagatggcaa aaacgccaga agcagcactc aactttatgc gggaaattgt
     6001 tccagcggcg cgtcaacgtg cgagcgatga attagtctcc atacaggcgg ttattgataa
     6061 gcagcagggc gggtttagcg cgcagccgtg ggactgggca ttttatgccg agcaggtaag
     6121 acgtgagaaa ttcgatctcg atgaatcgca gctcaagcca tattttgaat taaacacggt
     6181 gttgaatgaa ggtgtattct ggaccgcgaa tcagctcttc ggtattaagt ttgtcgaacg
     6241 ttttgatatt cctgtctacc atcctgacgt tcgtgtgtgg gaaatttttg atcataatgg
     6301 cgtggggctg gcgttatttt acggtgattt cttcgcccgt gattcaaaaa gcggcggtgc
     6361 atggatgggc aattttgttg agcaatcaac gctgaatgaa acgcatccgg taatttataa
     6421 cgtctgcaat tatcagaaat ccgccgctgg ggagcctgcg ttgttactct gggatgatgt
     6481 cataacctta ttccatgaat ttggtcatac gctgcacggc ctttttgccc gccagcgtta
     6541 tgccacgctt tccggcacca acacgccgcg tgattttgtc gaatttccgt cgcaaatcaa
     6601 cgaacactgg gcaacgcatc cgcaggtatt cgctcgctac gcccggcatt atcagagcgg
     6661 ggcagcaatg cctgatgaac tgcaacagaa aatgcgtaat gccagcctgt tcaataaagg
     6721 gtatgagatg agcgaactgc ttagcgccgc acttctcgat atgcgctggc attgcctgga
     6781 agaaaacgaa gcaatgcagg atgtcgatga ttttgaattg cgggcgctgg tggcggaaaa
     6841 tatggatctt cctgctatac cgccacgcta tcgcagcagt tatttcgccc atatttttgg
     6901 tggcggatat gccgcaggtt attacgctta tctgtggacg caaatgttgg ccgatgatgg
     6961 ttaccagtgg tttgttgagc agggcggatt aacgcgtgaa aatgggcggc gttttcgcga
     7021 ggcgattctt tccagaggta acagcgaaga tctggaacgc ctgtatcgac aatggcgcgg
     7081 taaggcacct cagattatgc cgatgctgca acatcgtggc ttgaacgtat aaacaccacc
     7141 aatggctggc ctggatttat cgccagacca gcctcgttat agcagtaatt gcagccgctg
     7201 ggtgagggca aacttcaccg ctaatgcgaa tgccgtttcg cagtcgccag caaaattact
     7261 ttttttgtat acccttccgt tataata
//
LOCUS       DAZUCC010000225         7212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZUCC010000225 DAZUCC010000000
VERSION     DAZUCC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.226"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..478
                     /gene="curA"
                     /locus_tag="ACHY09_004323"
     CDS             <1..478
                     /gene="curA"
                     /locus_tag="ACHY09_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFS3887206.1"
                     /translation="RHATEVLGFDVCLDHHADDFAEQLAKACPKGIDIYYENVGGKVF
                     DAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQDYG
                     HRIQEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNFGKVVIRVAVDD"
     gene            676..1341
                     /gene="mcbR"
                     /locus_tag="ACHY09_004324"
     CDS             676..1341
                     /gene="mcbR"
                     /locus_tag="ACHY09_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFS3887207.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(1377..3479)
                     /gene="pqqU"
                     /locus_tag="ACHY09_004325"
     CDS             complement(1377..3479)
                     /gene="pqqU"
                     /locus_tag="ACHY09_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFS3887208.1"
                     /translation="MKIFSVRQTVFPALLVLSPTVFAADEQTMIVSAAPQVISELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMSPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            3721..4782
                     /locus_tag="ACHY09_004326"
     CDS             3721..4782
                     /locus_tag="ACHY09_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFS3887209.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(4895..6394)
                     /gene="ansP"
                     /locus_tag="ACHY09_004327"
     CDS             complement(4895..6394)
                     /gene="ansP"
                     /locus_tag="ACHY09_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFS3887210.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            6661..>7212
                     /locus_tag="ACHY09_004328"
     CDS             6661..>7212
                     /locus_tag="ACHY09_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFS3887211.1"
                     /translation="MIKVHGVPGWGTTISEMMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFHDNAQNISS"
BASE COUNT         1937 a         1793 c         1758 g         1724 t
ORIGIN      
        1 ccgccatgct actgaggtgc tgggcttcga tgtttgtctt gaccatcacg cggatgattt
       61 tgccgaacaa ctggcgaaag cgtgcccaaa aggtattgat atctattatg aaaacgtggg
      121 cggtaaggta tttgatgcag tgctaccttt acttaataca tctgcgcgca ttcccgtttg
      181 tggcttagtg agcagctata acgctacaga gctaccaccc ggtccggatc gtttacctct
      241 gctgatggct acggtgctga aaaaacgtat tcgcttgcaa ggatttatta tcgctcagga
      301 ttatggtcac cgcatccaag agtttcagaa ggagatgggg caatgggtga aagaggataa
      361 aatccactac cgcgaagaaa ttactgacgg tttagagaat gcgccacaga cgtttatcgg
      421 cctgctgaag ggtaaaaact tcggcaaagt ggtgatccgc gtggcggttg atgattaaag
      481 tttcaacggc ggcgtaagcc gccataaaaa tccaggcaaa aagcttattt tcagctttaa
      541 ttaactaacc acgtcattgt tctgctgcta ataacaacaa aggtgagtgg tttatgatta
      601 ttcactttaa tacaccaggt gaactccttc tgccatgcag gcagggttgg acagaaaaca
      661 cgcgaaggaa ttaaaatgcc tggaacggaa aaaatgaaac atgtcagttt gactctgcag
      721 gttgagaacg acctgaaaca tcagcttagt attggcgcat taaaacctgg cgcacgcctg
      781 attactaaaa atctggcgga gcaattaggt atgagtatta cacctgtgcg tgaagcatta
      841 ttacgtctgg tttcggtgaa tgcgctttct gtcgcaccag cgcaagcatt tacagttccg
      901 gaagtgggga aacgtcaatt agacgaaatc aatcggatcc gctacgagct ggaattaatg
      961 gcagttactc tggctgttga aaacctcacc ccgcaagacc tggcggaact cgaggaattg
     1021 ctcgagaaat tacagcaggc gcaagaaaag ggcgacatgg aacaaatcat taatgcgaac
     1081 aggctatttc gcttagcgat ttatcatcgt tcaaatatgc ccatcctgtg tgagatgatt
     1141 gagcaactgt gggtcaggat gggacctggt ttacattatc tctatgaagc gattaatcca
     1201 gcggaattac gggagcgtat agaaaactat cgtctattac tcgccgcgtt aaaagcaaaa
     1261 gacaaagagg gatgcagaca ttgtcttgct gaaattatgc aacaaaatat tgctatttta
     1321 taccagcaat acaatcgtta aattaaatgc ccggcaaata ccgggcatca aggtaattac
     1381 tcaaatcgcc acgcaatatt catgccgaca ccataatttc gcccgggcgc aggttcgtaa
     1441 tatcgcccgt ttgactcatt gacaatgaca gagccaacgt attctttatc gaataaatta
     1501 tcgacacgac caaataaatc cacagttaaa ttgtggtaat tgtatttata cccggtgaat
     1561 aagccgacga gagtataaga cggcgctttg gccgtatttt catcatctgc cataatatcg
     1621 cccatataac gcgcttccgt acctgcatac cagccatctt ccggtacata acctatcgac
     1681 gcaaagccca tattacgggc gatccctggc atccggttac cgttacaatc ctgttcattg
     1741 caaacattgc tgcgataggt cgcatcaagc caggtccagg acgcttttac gcgaaaatct
     1801 cccgcgaaac gttgatccca tgccagttca gcgccttgac gacgggtctt tccggcattt
     1861 ttgtaagtcg tacgcccacc gctactgcta tcgacaacaa tttcatcatc ggtgtcggtc
     1921 tggaacaatg cgagactaag cagcccatca ccaatacgcg ttttactgcc gatctcaatt
     1981 gtatcgttgg tggagggttt taaaccaaag ttcataccgc tttgcccatc agcgcgataa
     2041 gacagctcat taatcgtcgg cgtttcaaaa ccgcgcccgg ctgccagata gacattccag
     2101 gcatcggtca ttgcatattt taacgatccg gctggtagcc atttgtgata actggcatca
     2161 ccgctgtcgt cgccgttacc cggagtaacg taatgatcat tggaatcaaa ccacacagag
     2221 ctgtagcgca cgccagcatc cagcgaaagt ttgtcggtca gttgccactg ggtttgtaaa
     2281 tagggatcga cgttccacat caaattgcgt tcgtcgcggc gcaactcacc tttttgcccg
     2341 tattccggca cgccgcgatt caggcggaag ttattgtaac ccttgcggtt ttcactcatg
     2401 ttttcgtagt tcaggccggt tgtgaacgtg actggaacgc ccaattcacc acggtgtgtc
     2461 cagcggctgt ctatcccctg gtaatggcgt tgcagggtaa tcacgccgcc cgcatgtgac
     2521 gggttaagtt gcggggacat ggggattgac tggtactggg tcgtttctcg ctctccggca
     2581 tacatcatta cactcatatc atcttgcgcg ctcaggctac gttcatagcg caacccagcc
     2641 tgagtttgct tgatggtttt tcgcgtgtcg tactgttctg cacggggcgc ttgttgtgga
     2701 ttcgccttcc attctgcttc ggttagccca cctgggtcat ctgctttgat atccacacta
     2761 ttgaaaatca ggcttaattt gctggcgtca tcaatgcgaa cgcccagttt ggcattggct
     2821 aaatttttct gtgcgccact atgatcacga tagccgtggg tcgtaaagcg cgtggttgag
     2881 acggtgtaat cgacatcgcc tggctgtgtg ccgtctcccg ttgcgcccgt tgctttcagc
     2941 ccatagcgcc agctgccaaa actgccgtag taactactgg cttcaatggt tggtggctgt
     3001 tgtccggtct gggtgatgac attcattaca ccgccagacg cgttgccata cagagcagag
     3061 aaggggccac gcagcacttc cacattttgc acactgctta aatcgatatt ggatgtctgc
     3121 ccttgtccgt cgggcatggt agcgggaata ccgtcaacat acaggcgaat accgcgaatt
     3181 ccgtaagtgg agcgggagcc aaatccgcga atcgacagct gtaaatcttg cgcatagttc
     3241 tgccggtttt gtacctgcaa accaggcacg ccagtcagtg attcagacaa gttaatgcgc
     3301 ggtgttgcca ggcgcatctc ctcgccatcc accacgctca cggctgctgg ggtatccagt
     3361 tctgaaatca cctgcggcgc ggcactgaca atcatggtct gttcatcagc ggcaaaaacg
     3421 gtgggggaaa ggacaagcag cgcgggaaaa acggtctgtc ggacggaaaa aatcttcatg
     3481 aaaaaagcca ggttaagaat gggaaaacac cgtcatggta ataaaattgt aaatttatgg
     3541 aaaacgaaac ggcacgatat gttaagtaat tgagaaaatt gtagtcgtaa cggcaagaaa
     3601 tgctccacat ttgagaaaat aatgattacc attcccattt ataacaagag cgtaacgatg
     3661 attacgctta gcgaagcatt gtgaagcagc aaaaatatcg gttcatcaaa gggagtcgtc
     3721 atgcatttac gtcatctgtt ttcatcgcgc ctgcgtggtt cattactgtt aggttcattg
     3781 cttgttgctt catcattcag tacgcaggcc gcagaagaaa tgctgcgtaa agcggtaggt
     3841 aaaggtgcct acgaaatggc ttatagccag caagaaaacg cgctgtggct cgccacttcg
     3901 caaagccgca aactggataa aggcggcgtg gtttatcgtc ttgatccggt tactctggaa
     3961 gtgacgcagg cgatccataa cgatctcaag ccgtttggtg ctaccatcaa taacacgact
     4021 cagacgttgt ggtttggtaa cactgtaaac agcgcggtca cggcgataga tgccaaaacg
     4081 ggcgaagtga aaggccgtct ggtgctggat gatcgtaagc gcactgaaga ggtgcgcccg
     4141 ctgcaaccgc gtgagctggt ggcagacgat gccacgaaca ccgtttacat cagtggtatt
     4201 ggtaaagaga gcgtgatttg ggtcgttgat ggcgagaata tcaaactgaa aaccgccatc
     4261 cagaacaccg gtaaaatgag taccggttta gcgctggata gcaaaggcaa acgtctttac
     4321 accactaacg ctgacggcga attgattacc atcgacaccg ccgacaataa aatcctcagc
     4381 cgtaaaaagc tgctggatga cggcaaagag cacttcttta tcaacatcag ccttgatacc
     4441 gcaaggcagc gtgcatttat caccgactct aaagcggcag aagtgttagt ggtcgatacc
     4501 cgtaatggca atattctggc gaaggttgcg gcaccggaat cactggctgt gctgtttaac
     4561 cccgcgcgta atgaagccta cgtgacgcat cgtcaggcag gtaaagtcag tgtgattgac
     4621 gcgaaaagct ataaagtggt gaaaacgttc gatacgccga ctcatccgaa cagcctggca
     4681 ctgtctgccg atggcaaaac gctgtatgtc agtgtgaaac aaaaatccac taaacagcag
     4741 gaagctaccc agccggacga tgtgattcgt attgcgctgt aatagatatg gcgagggtga
     4801 aaatggagag acttgcctga cttttatcac gccgtatccg gcaccttcgt cagtaatttg
     4861 aatttgcccg gcacgtagcc gggcaatttt gcaattagct cgccgtttca ggcttaaaca
     4921 caatttcctg tttttcttca tcttcctcga cgactggcgc agtgctgtga atttcagcaa
     4981 cgcgtttgcg cacaccaaac cagcctataa ccagcagaat accgataatc ggcagcgccg
     5041 cgatagtata agtcccgttc gggtaatcga acgccatcag cacaaggacg ctcagcaaaa
     5101 acagtaatgt cagccaggaa gtgaagggcg cgccaggcag tttaaaactg acatccgctg
     5161 ctttgccttc tttaatcgct ttacgcaggc gcatctggca cacgatgata aacgcccatg
     5221 aagcgatgat tcccagcgac gcgaagttca acacaatctc aaatacgcgc gacggcacca
     5281 gatagttgag gaatacgccg acgacataca caaccagtgt cgccagaatc ccggcatacg
     5341 gcacatgctg acgactcatt ttcgccataa aactcggtgc ggaaccgccc atcgccatcg
     5401 agcgcagaat acgtccggtg cagtacagac ctgagttcag gctggagagg gcagcggtca
     5461 gcaccacaat gttcataatg ctgccgatgt acggcacacc cagtttagag aaaaacgtca
     5521 cgaacggact ttgccccgcc tgatacgcgc tccacggcaa taacataacc agcaacacca
     5581 cggagccgac gtaaaacagg ccaatacgcc aaatcacact gttaatcgct ttaggcacca
     5641 tggtctgcgg atctttacat tctccggcag ctgtacccac catttcaatg gaggcaaaag
     5701 caaacactac gccctgaatc aacaccagcg caggtagcaa accgtggggg aagaagccgc
     5761 cgttatcggt aattaaatga aagccagtgg tgttgccatc cagcggctga ccactaccga
     5821 ggaacactgt acccacgacc aaaaaggtca caatggcgag cactttaata agcgcaaacc
     5881 agaactccat ctcggcaaac catttcacac caatcatatt catggtgcca acgatggtca
     5941 gcgcagcgag cgcaaagacc cactgcggta caccaccaaa tgcgccccag taatgcatat
     6001 acagtgcaac ggcggtaata tcgacaatcc ccgtcatcgc ccagttgatg aagtacatcc
     6061 agcctgcaac ataagcggct ttctcaccca aaaactcacg ggcataagag acaaaactgc
     6121 cactggaagg gcggtgtagc accagctcac ccaatgcacg cagaataaaa aacgaaaaca
     6181 agccgcagat taaatagacc agtgccagtg ctggccccgc catttgcagt cgggctcccg
     6241 cgcctaaaaa caaacccgta ccaatcgcgc cgccaatggc gatcatctgc acctggcgat
     6301 tgcccatcgc tttgtgatac ccctcttcgt gggcattaag ccagcggcgt ttcgcggcgt
     6361 gctgatctga agtgtcggtg tcgtgtttac tcattgctct ccctgattgc tttaatgaaa
     6421 aagtcatata agttgccatg aacaatgttt attcatcctg gcgatgatag ttatcaccat
     6481 tattctttat gggtataaaa ctatcggcgc agcatcctac ccgttatcga taaacgatgc
     6541 aaaacatccc cttacaattc tgaaggggat tcatacaact gacgaaaaaa tgacaaatac
     6601 ttttgctggt taacctgtgt gctgtcctag acttaatctt taaaagattg tgaggggcat
     6661 atgattaagg tgcatggcgt acccggatgg ggcactacaa tcagtgagat gatgctaaca
     6721 ctggctgata ttccttatca gttcgttgat gtcagtggtt ttgaccacga gggaacttca
     6781 cgcgagttat tgaaaaccct gaacccgttg tgtcagattc cgacactggc gctggaaaat
     6841 gacgagatta tgacggagac agcggcgatt gcattgatgg ttctcgatcg tcgcccggac
     6901 cttgcgccac cggttgggcg cgccgaacgt cagcagtttc aacggctatt ggtctggctg
     6961 gttgctaatg tctatccaac attcactttc gccgattacc ctgagcgttg ggctcctgac
     7021 gcacccgaac agttaaagaa taatgttatt gaatatcgta aatcgcttta tatctggctg
     7081 aattcacaat tgaccgctga accttatgcg tttggtgagc aactaacgct ggtggactgt
     7141 tatctttgca ccatgcgcac atgggggccg gggcatgaat ggtttcacga taatgcgcag
     7201 aatatcagct cg
//
LOCUS       DAZUCC010000226         7068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZUCC010000226 DAZUCC010000000
VERSION     DAZUCC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.227"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1792
                     /locus_tag="ACHY09_004329"
     CDS             <1..1792
                     /locus_tag="ACHY09_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFS3887212.1"
                     /translation="DKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIVDP
                     YGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQKF
                     ANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLYLV
                     NTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLDQD
                     TFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFADAT
                     HESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDPKR
                     DALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAKDD
                     SIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGLCE
                     NSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGTVQ
                     QVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYKTK
                     EVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1810..2478
                     /locus_tag="ACHY09_004330"
     CDS             1810..2478
                     /locus_tag="ACHY09_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFS3887213.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSLA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2493..3164
                     /gene="dsbI"
                     /locus_tag="ACHY09_004331"
     CDS             2493..3164
                     /gene="dsbI"
                     /locus_tag="ACHY09_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFS3887214.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIVRG"
     gene            complement(3210..3998)
                     /gene="ygiD"
                     /locus_tag="ACHY09_004332"
     CDS             complement(3210..3998)
                     /gene="ygiD"
                     /locus_tag="ACHY09_004332"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFS3887215.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4141..4914
                     /gene="zupT"
                     /locus_tag="ACHY09_004333"
     CDS             4141..4914
                     /gene="zupT"
                     /locus_tag="ACHY09_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFS3887216.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(4972..5142)
                     /gene="yqiD"
                     /locus_tag="ACHY09_004334"
     CDS             complement(4972..5142)
                     /gene="yqiD"
                     /locus_tag="ACHY09_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFS3887217.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(5404..6057)
                     /gene="ribB"
                     /locus_tag="ACHY09_004335"
     CDS             complement(5404..6057)
                     /gene="ribB"
                     /locus_tag="ACHY09_004335"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFS3887218.1"
                     /translation="MNQTLLSSFGTPFERVENALVALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(6161..6309)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6431..6730
                     /gene="ubiK"
                     /locus_tag="ACHY09_004336"
     CDS             6431..6730
                     /gene="ubiK"
                     /locus_tag="ACHY09_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFS3887219.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(6765..6965)
                     /gene="glgS"
                     /locus_tag="ACHY09_004337"
     CDS             complement(6765..6965)
                     /gene="glgS"
                     /locus_tag="ACHY09_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFS3887220.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT         1866 a         1766 c         1687 g         1749 t
ORIGIN      
        1 tgataaatat agaaaaacac tcgtagccgg aactgtggcg ataaccctgg gtttgtcagc
       61 atcgggggtg atggctgcgg gttttaaacc agcgccgcct gccgggcaac tgggtgcggt
      121 cattgtcgat ccctacggca atgcaccact gaccgctttg gttgacttag atagccatgt
      181 tatttctgac gtcaaagtta ccgtccatgg gaagggcgaa aaaggtgtag aaatcagcta
      241 tcccgtgggt caggaatcac taaaaactta cgatggtgta ccgatttttg gtctttatca
      301 gaaatttgct aacaaagtga ccgttgagtg gaaagaaaac ggcaaggtca tgaaagatga
      361 ttatgtggtg cacacttcgg ccatcgtcaa taattacatg gataaccgct ctatatccga
      421 tttacaacag accaaagtta ttaaagtcgc accgggtttt gaagatcgcc tctatctggt
      481 taatacccac acctttaccg cccaaggttc cgatctccac tggcatggtg agaaagataa
      541 aaatgccggt atccttgatg cgggtccggc aaccggcgca ctaccttttg atatcgcgcc
      601 ctttactttc atcgtcgata ccgaaggtga ataccgctgg tggctggatc aagacacttt
      661 ctacgatggt cgtgaccgcg atatcaacaa gcgtggttat ctgatgggta tccgcgaaac
      721 gccacgcggc acctttaccg ctgtacaagg tcagcactgg tacgagttcg acatgatggg
      781 gcaggtgctc gaagatcaca aactaccgcg cggatttgct gacgccactc atgagtccat
      841 tgagacgcca aatggcacgg tactgttgcg cgtaggtaag agtaactatc gtcgcgatga
      901 cggcgtacac gtcaccacca ttcgtgacca tatcctcgaa gttgataaat ctggtcgcgt
      961 tgtcgatgta tgggatctga ccaagatcct cgatccgaaa cgcgatgcac tgctcggcgc
     1021 gctggatgca ggtgcagttt gcgttaacgt tgaccttgcc catgcaggac aacaggcaaa
     1081 actggaacca gatacaccgt tcggcgacgc gctgggtgta gggccaggcc gcaactgggc
     1141 gcacgtgaac tctatcgcct atgacgcaaa agatgactct atcatcctct cctctcgcca
     1201 ccagggtgtt gtgaaaattg gtcgtgataa gcaagtgaaa tggatccttg cgccctctaa
     1261 aggttgggaa aaaccgctgg ccagcaagct gctgaaaccg gttgatgcta acggtaagcc
     1321 aattacctgt aacgaaaatg gcctgtgcga aaactcagac ttcgacttta cctacaccca
     1381 gcataccgcc tggatttcca gcaaaggaac gctcaccatt tttgataatg gcgatgggcg
     1441 tcatctggaa caacctgcct taccaaccat gaaatactcc cgctttgtgg aatataagat
     1501 tgatgagaag aaaggcaccg ttcagcaagt gtgggaatac ggtaaagaac gtggctacga
     1561 tttctatagc ccgattacct ccatcattga atatcaagcc gaccgtaaca ccatgtttgg
     1621 cttcggtggt tctattcatt tgttcgatgt cgggcagcca accgtcggta agttgaacga
     1681 aatcgattac aaaaccaaag aagtgaaagt ggaaatcgac gtgctgtcag ataaacccaa
     1741 tcagactcac tatcgtgcac tgttagtccg tccacaacag atgttcaaat aattaagaag
     1801 ggaataaaaa tgtcgaaatt agggatctca tcactattta aaaccatact gcttaccgca
     1861 gctttggctg tttcatttac cgcttctgca tttaccgaag gcacggatta catggttctg
     1921 gaaaaaccta ttccgaatgc cgacaaaacg ctgattaagg tattcagcta cgcctgcccg
     1981 ttctgttaca agtacgacaa agcggttacc ggtccggtat cggaaaaagt gaaagatatc
     2041 gttgctttca cgccgttcca tctggaaacc aaaggtgaat acggcaaaca agcaagcgaa
     2101 gtctttgcgg tcttgatcaa taaagataaa gcagcaggca tttccttatt tgatgctaac
     2161 tctcagttta agaaagccaa gtttgcctac tacgcggctt atcacgacaa aaaagaacgc
     2221 tggtctgacg gtaaagatcc ggctgctttt atcaaaaccg gtctggatgc cgcaggtatg
     2281 agcttggcgg attttgaagc tgcactgaaa gaaccagcgg ttcaagaaac actggaaaaa
     2341 tggaaagcct cttatgatgt cgccaaaatc cagggggttc cagcctatgt cgttaatggt
     2401 aagtacttga tctacaccaa gagcatcaaa tccatcgacg cgatggcaga ccttatccgt
     2461 gagttagcaa gcaaataaca gggaaacagg ctatgggtat taagggaatg tggaaagacc
     2521 ttcgcacatc accagttgac acactggtca gatggcagga gcaacgcttg ttgtggttgt
     2581 taatggccgt tgcgatgggc gcgttaatta tcctggcgca ctctttcttc cagatctatc
     2641 tctacatggc accctgtgag caatgtgtat acattcgcta cgccatgttt gtgatggtta
     2701 ttggaggatt ggttgcagcg atcaacccaa agaacatcat cctaaagcta attggctgcg
     2761 tgatggcatt ttacggcagc attttgggac tgaagttttc gctcaaacta aacgatatcc
     2821 accatgcagt acataatccg gatcccgatt cactgtttgg cgtacagggt tgctctaccg
     2881 atccaacttt tccctttaac ctgccgctgg cacagtgggc accaaattgg ttcaagccta
     2941 caggggattg tggctatgac gcaccaatcg ttcctgatgg cgtcacactg agcagcacgc
     3001 aacagtggtt tgtggaaatg tatcagcaat ctgaaggctg gtatctgcta ccaccatggc
     3061 attttatgaa tatggctcag gcttgtatgc tggcttttgg tatgtgtctg gtgctgctgg
     3121 tcattatgag tggcgcatgg gcgcttaaaa tcgtcagagg ctaactcagc aacacccgaa
     3181 gccagtcgtt ctggcttcgg gttcttttat tagcctatct gcaccgacaa catacttaaa
     3241 ctgcccattt ctataccatc gaccggaatg gtaattggct cctgcccatc ccacgcacct
     3301 aagacataca acaacggcag atagtgctct ggcgttgggt tcgataacgc gccaccttca
     3361 tggtcgagat aattcaccag aggatgctgc tctaccggcc cttgccacgt cagattcgct
     3421 ttcacatact cattaaacga cgtcgcccac ggatacggtg aactatcacc gtgccacttc
     3481 actgtgcgca ggttatgcac cacgttaccg ctggcgacca acattattcc ttcatctcgc
     3541 agcgccgcga gtttgcgccc catttcgaaa tgccaggcgg caggtttgct actgtcgata
     3601 ctcaactgca ccatcgggat atccgcgtca ggatacatct taatcagcac gccccaggaa
     3661 ccgtggtcaa agccccaggc ttctttatcc agcgtcaccg ggatcggcgc taacagctca
     3721 accagacgct gtgccagcgc aggcgaaccc ggagcaggat aatgcgtatc gtacagcgcc
     3781 tgcgggaagc caccaaagtc atgaatcgtg ggcggcgtct ccatcgcggt cactcctgtt
     3841 ccacgggtaa accagtgagc cgaaaccacc acaatcgctt gcgggcgtgg caatgtcatc
     3901 cccaacgtct gccagctgcg ggtatacaaa ttatcttcca gcacgttcat cggactaccg
     3961 tgacctaaaa acaatgctgg catacgtgtt gaagacatga tgatatcctt aactaaaggt
     4021 gtcattttga tatcctcaca atacgcttgt tcggcggagt aagaacccgg ataacaatga
     4081 tgatgatcat cagttatttt gacgatctgc ctgaaggtga agatttataa ggagttgtcg
     4141 atgtcagtac ctctcattct gaccatactg gcgggggcag ccacgtttat tggcgcgttt
     4201 ctcggcgttc tcgggcaaaa accctcgaac cgcttactgg cgttttcgct aggatttgcg
     4261 gcggggatca tgttgctcat ctcattaatg gaaatgcttc ctgccgcact agccgccgaa
     4321 ggaatgtcgc ctgtgttggg ttatggaatg tttatcttcg gtctgcttgg ctattttggc
     4381 ctggaccgca tgttgccaca tgcgcatccg caggatttaa tgcaaaaatc ggtgcagccg
     4441 ttgccaaaat cgatcaagcg cacagccatt ctgctcactc tcggcatcag tctgcataac
     4501 ttcccggaag ggattgccac ctttgtcacg gcgagcagca acctggagct gggctttggc
     4561 atcgcactgg ccgttgcgtt acacaatatt cctgaaggtc tggcggttgc aggcccggtt
     4621 tacgcggcaa cggggtctaa acgcaccgca attctttggg cggggatttc tggactggca
     4681 gaaattcttg gtggtgtgct ggcgtggtta atcctcggta gcatgatttc cccggtggtc
     4741 atggcggcaa tcatggcggc ggttgcagga attatggtgg cgctctcggt tgatgaatta
     4801 atgccgctcg ccaaagagat tgaccctaat aataacccca gctatggcgt actatgtgga
     4861 atgtcagtga tgggattcag tttagtgctg ctacaaacgg cgggaattgg ttaaaagcaa
     4921 catcagaagt tgtccagcgc atggacaact tctgccagct tcactaaagc cttattctac
     4981 cttttcggct gtctcttctc tcgtactgtt taagtatttg ttccgcgctt caagcagtgc
     5041 gtctctgtcc tggcgaaacg cccgacaata acgtttcaaa tgcaggaaat aacccaccac
     5101 aacaagagca atcaatacaa tccagtacca ggcgataaac atccttttac ccttaatatt
     5161 taacaatact aaacatttag cgcataaatt tcacatatcc tttttcggat atattcatat
     5221 ggtcgcataa taacggaaca attatatgat tttgatcata atgaaaaaga atgcctgata
     5281 tcaatgaaat tttctcatag aaagagaagc ggaattaaca tcttgcatag cagggcaaaa
     5341 taaaaaccga agccagctac aacttcggtt tcttgattaa gacagtaaat taagcagcga
     5401 ttttcagctg gctttgcgtt catgcgcctg acgataagcc accaggtctt caatagtcac
     5461 gagcgccata ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc
     5521 atcgtcatta gtcagctcac acagcacacc agccggttta aagccagcca gcgtcatcag
     5581 atcaatagtc gcttcagtat ggccgccacg cgtcagtaca ccaccagcct gagcgcgaag
     5641 tgggaaaacg tggcccgggc gattcagatc agaaggtttt gcgccatcgg caatcgctgc
     5701 gcgaacggtc gtaatacggt cagcggcaga aacaccggta gtcacacctt cagctgcttc
     5761 aatggtcacg gtaaaaccgg tgccataggc gctggtgtta ttttctacca tcattggcag
     5821 atcgagttgt ttacggcgat cttcagtgat gcacaggcaa acaataccgc taccgtggcg
     5881 aatggtcagc gccatctgct caacggtcat ggtttctgcc gggaagatca tatcaccttc
     5941 gttttcacgg tcttcatcat caagcaccat tacaccgcgt ccttcacgca gcgcaaccag
     6001 tgcattttca acacgttcga aaggcgtacc aaaagaggaa agtagcgtct gattcatggt
     6061 aaaaaaacct cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat
     6121 atagccaaaa taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg
     6181 actctaaccg tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa
     6241 gcgctcgcgg gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg
     6301 agaataagcg gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata
     6361 attctggttt atgacttacc cttatcgcac tacaatggca ctcaacacct atcagtacag
     6421 ggaaactaca atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc
     6481 aatgcctaaa ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct
     6541 acaagcgcag ctgacgcgtc tcgatctggt aagccgcgaa gaattcgacg ttcagacgca
     6601 agtcctgtta cggactcgtg aaaaactggc gttactggag cagcgcatca gtgaactgga
     6661 agcgcgcaat aattcggtag cagatcttca atctcctccg gctatcccgc ctattgataa
     6721 agcagaataa agcatcacaa aaacgggcca gacggcccgt aatatcagtg ttctaattca
     6781 agttctctgg tttgcatcat ctgttggcaa taccaggcat aacgtgcgca aaaccaggtt
     6841 ctatgttctt catccatgtt accagtaacg gccagaatat cgaccgggcg accttctgcg
     6901 tgcattctgg caaaactacg cgccaggaaa tcgaaattat ttaaagaatt aagactatga
     6961 tccataagca catcctccgg ttatcgcata ccttttaagg ccatatgctt tttctgattt
     7021 cagtattccc caatcagtgt agtgccttta aacataacgt gcgtaaat
//
LOCUS       DAZUCC010000227         7046 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZUCC010000227 DAZUCC010000000
VERSION     DAZUCC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.228"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..234
                     /locus_tag="ACHY09_004338"
     CDS             <1..234
                     /locus_tag="ACHY09_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3887221.1"
                     /translation="HWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGLRHIAINILT
                     NDKVFKAGLRRKLRKAAMDRNYLASVLAGSGLS"
     gene            432..2669
                     /gene="nrfB"
                     /locus_tag="ACHY09_004339"
     CDS             432..2669
                     /gene="nrfB"
                     /locus_tag="ACHY09_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFS3887222.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNPEQVAQLES
                     ENEGE"
     gene            2656..5628
                     /gene="nfrA"
                     /locus_tag="ACHY09_004340"
     CDS             2656..5628
                     /gene="nfrA"
                     /locus_tag="ACHY09_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFS3887223.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHGMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRH
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPRKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGIEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5629..6519
                     /locus_tag="ACHY09_004341"
     CDS             5629..6519
                     /locus_tag="ACHY09_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFS3887224.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
BASE COUNT         1752 a         1821 c         1822 g         1651 t
ORIGIN      
        1 cactggcacg tggagaataa gctgcactgg cgtctggacg tggtaatgaa tgaagacgac
       61 tgcaaaataa gaagaggaaa tgcagcagaa ttattttcag ggctacggca catcgctatt
      121 aatattttga cgaatgataa ggtattcaag gcagggttaa gacgtaagtt gcgaaaagca
      181 gccatggaca gaaactatct cgcgtcagtc cttgctggga gcgggctttc gtaatctttc
      241 cctgcgttaa cctcccctcc ttgacaccac gcttttgtct acattctacg caaacggcaa
      301 cttttataac cagtgtaaaa ataacgtgcc gcaatagtca tacctattaa tggtaaaaag
      361 ctgtcacaat tcataaaaaa ccttaatata cgccacccta aacataacca gcgttaatgt
      421 aaggtttttg tgtggactgg cttcttgatg tttttgctac ctggctctac ggcttaaaag
      481 taatcgcgat aacgttagcg gtcatcatgt tcatcagcgg gctggacgat ttttttattg
      541 atgtcgtcta ctgggtacgc cgcattaaac gcaagttgag tgtttatcgc cgctacccgc
      601 gaatgagtta ccgcgaactg tataaaccag atgaaaaacc gttagcgatt atggttccgg
      661 cgtggaatga aacgggcgtc atcggcaata tggccgagct ggcggcgacc acgctcgact
      721 acgaaaacta tcatatcttt gttggcacct accccaacga ccccgatact cagcgtgatg
      781 ttgacgaagt gtgcgctcgc ttcccgaacg tgcataaggt agtctgcgcg cgtcctggcc
      841 ccaccagcaa agccgactgt ctgaacaacg tgctggacgc catcacccaa tttgaacgta
      901 gcgccaattt cgcttttgct ggttttattc tgcatgacgc cgaagatgtg atttcaccga
      961 tggaattgcg tctgttcaac tatctggtcg agcgtaaaga tctgattcag atcccagtgt
     1021 atccgttcga acgcgaatgg acgcacttca ccagcatgac ctacattgat gagttttcag
     1081 aactgcatgg caaagatgtt ccggtgcgtg aagccctcgc cggacaggtg cccagcgcag
     1141 gcgtcggcac ctgtttcagc cgccgcgccg tgaccgcact gttagctgac ggtgacggta
     1201 ttgctttcga cgtgcagagt ctgactgaag attacgacat tggtttccgc ctgaaagaaa
     1261 aaggtatgac ggaaattttt gtccgttttc cggtggtgga cgaagccaaa gaacgcgagc
     1321 agcgtaaatt tttacagcac gcgcggacgt caaacatgat ctgcgtgcgc gaatatttcc
     1381 ccgatacctt ttcgactgcg gttcgacaaa aatctcgctg gatcatcggc attgttttcc
     1441 agggctttaa aacccacaaa tggacctcca gcctgacgct gaactacttt ctctggcgcg
     1501 accgcaaagg ggcaatcagt aactttgtca gcttcctcgc gatgctggtg atgatccagc
     1561 ttttgctgtt actggcgtat gaaagtttgt ggcccgatgc ctggcatttc ctttctattt
     1621 tcagcggcag cgcatggtta atgaccctgc tgtggctaaa ctttggtttg atggttaacc
     1681 gcatcgtgca gcgggtgatt tttgtcactg gctactacgg tctgacgcag gggctgctat
     1741 ctgtcctgcg tcttttctgg ggcaacctga ttaacttcat ggccaactgg cgcgcgttaa
     1801 aacaggtact tcaacacggc gatccacgtc gcgtcgcgtg ggataaaaca acgcatgact
     1861 tccccagcgt gactggcgat acccgctcgt tgcgcccgtt aggccaaatc ctgctggaaa
     1921 atcaggtcat cactgaagaa caactcgata cagcactgcg taatcgcgtc gaaggtctac
     1981 gcctgggcgg ttcaatgctg atgcaggggc tgattagcgc cgaacagctg gcacaggcgc
     2041 tggcagagca aaacggcgtg gcgtgggagt cgatcgacgc ctggcagatc ccttcatcac
     2101 tgattgccga aatgccggct tccgtggcgc tgcattatgc agtgctgccg ctgcgtctgg
     2161 aaaatgatga gttaattgtc ggcagtgaag atggtattga cccggtttcg ctggcagccc
     2221 tgacgcgtaa agtcggacgc aaagtgcgtt acgtcattgt tctgcgcgga caaattgtca
     2281 cggggttacg tcactggtat gcacgccgac gcggtcacga cccgcgggca atgttgtaca
     2341 atgcggttca gcatcagtgg ctcacggaac agcaggccgg tgaaatctgg cggcaatatg
     2401 tgccgcatca gttcctgttc gccgaaatac tgaccacgct cggtcatatt aatcgttcag
     2461 caattaacgt gttgttattg cgccatgaac gcagttctct gccgctcggc aagtttttgg
     2521 tcaccgaagg cgttatcagc caggaaacgt tggatcgcgt cctgacaatt caacgcgaat
     2581 tacaagtttc gatgcaatca ctattactca aagcaggttt aaacccagaa caggttgcgc
     2641 aactggagtc cgaaaatgaa ggagaataac cttaatcgcg tcatcggatg gtctggttta
     2701 ctgctgacgt ctttattgag caccagcgca ctcgcagaca atatcggcac cagcgcagaa
     2761 gagctggggc tgagcgatta tcgccatttt gttatttatc cccgtctcga taaggcgctg
     2821 aaggcacaga aaaataacga cgaagcaacc gccatccgcg aatttgaata tatacaccaa
     2881 caggtgccgg ataatattcc gctgacttta taccttgcgg aagcctatcg ccattttggt
     2941 catgatgacc gggcgcggct gttgcttgag gatcaactga aacgtcaccc aggagatgcc
     3001 cgacttgagc gcagtctggc ggctattcca gttgaagtga aaagcgttac gactgttgaa
     3061 gaactgcttg cccagcaaaa agcgtgcgat gctgcgccga ccctgcgttg tcgcagtgaa
     3121 gtcgggcaga atgccctgcg gctggcacaa ttacctgtcg ccagagcgca actgaacgat
     3181 gcgacgtttg ctgcatcgcc ggaaggaaaa acgctgcgaa ccgatctgct gcaacgggca
     3241 atctacctga aacaatggtc ccaggcagat acgctataca atgaagcacg ccagcagaac
     3301 acattaagcg cggcagaacg ccgtcagtgg tttgatgtgc ttcttgccgg gcagctggac
     3361 gatcggatcc tggcgctgca atcacagggg atcttcaccg atcctcagtc atatattact
     3421 tacgcgaccg cgctggctta tcgtggcgaa aaagcacgcc tccagcatta tctcattgaa
     3481 aatacgccgc tatttaccac ggacgcacaa gagaaaagtt ggctctatct gttatctaaa
     3541 tacagcggca accccgttca ggcgttggcg aattatacgg tacagtttgc cgacaaccgc
     3601 cagtatgttg ttggcgcgac gctaccggtg ctgttaaaag aaggtcagta cgacgcagcg
     3661 caaaaactgc tcgccaccct ccccgccaat gaaatgcttg aggagcgtta taccgttagc
     3721 gtggcgaccc gtaacaaggc tgaagctctg cgtctggcac gattgctgta tcagcaagaa
     3781 ccggcaaatc ttacccgcct ggatcaactg acctggcaac tgatgcagaa cgagcagtca
     3841 cgtgaagctg ccgatttatt gctgcaacgc tatcctttcc agggcgatgc gcgtgtcagc
     3901 cagactttaa tggcgcgact ggcgtctctg ctggaaagtc atccttacct ggcaactccg
     3961 gcgaaggtgg cgattttatc gaaaccctta ccgctggcgg agcaacgtca gtggcaaagt
     4021 cagttgccgg gtattgcaga taattgcccg gcaatagttc gcttgctggg cgatatgtcg
     4081 ccttcctacg atgctgccgc ctggaaccgt ctggcaaagt gttatcggga cacgctaccc
     4141 ggtgtggcgt tgtatgcatg gcttcaggcc gaacaacgac aaccgaacgc ctggcaacat
     4201 cgtgcggtag cctatcaggc gtatcaggtt gaggactacg ccaccgcact ggcggcctgg
     4261 cagaaaatca gtcttcacgg catgagcaat gaggatctgc ttgctgctgc caataccgcc
     4321 caggcggcag gaaatggtgc ggctcgcgat cgctggcttc agcaggcaga acaacgtggg
     4381 ctgggaaaca atgccctcta ctggtggctg catgcgcaac gttacattcc tggtcagccg
     4441 gaactcgcac tgagcgatct cacgcgctca atcaatattg cgccttctgc caacgcttac
     4501 gttgcgcggg cgacaattta tcgccaacgt cataatgtcc cggcggcggt gagtgatttg
     4561 cgcgccgctc tggaactgga accgaataat agcaacaccc aggcagcgct cggttacgcc
     4621 ttgtgggata gcggtgatat cgcacagtcg cgggaaatgc tcgaacaggc gcataaaggg
     4681 ctaccggacg atccggcact tatccgacaa ctggcctacg tgaaccagcg tctggatgac
     4741 atgcctgcga cgcagcacta cgcccgactg gtgattgatg acattgataa tcaggcgctg
     4801 ataaccccac tgaccccaga gcaaaatcag caacgcttca atttccgccg tctgcatgag
     4861 gaggtcggtc gccactggac gttcagtttc gattcttcta ttggcttgcg ttccggcgca
     4921 atgagtaccg ctaacaataa tgtcggcggc gcagcgccaa ggaaaagcta tcgtagctac
     4981 gggcaactgg aagccgagta ccgcatcgga cgcaatatgc tgctggaagg cgacctgctc
     5041 tcagtttata gccgcgtctt tgccgatacc ggtgaaaacg gggtgatgat gccggtgaaa
     5101 aatccgatgt ccggcaccgg tctgcgctgg aagccgctgc gcgatcagat ctttttcctc
     5161 gccgtcgaac agcagttgcc gctgaacgga caaaatggcg catccgatac catgctgcgc
     5221 gccagcgcct cattctttaa tggcggcaaa tacagcgacg aatggcaccc gaacggttca
     5281 ggctggtttg cccaaaacct gtacctcgat gcggcgcaat atatccgcca ggatattcag
     5341 gcgtggacgg cagattatcg agtcagctgg catcagaagg tggcaaacgg gcagactatt
     5401 gagccttatg ctcacgttca ggacaacggt tatcgtgata aaggcactca gggcgcgcag
     5461 cttggcggtg tcggggtccg ctggaatatc tggaccggcg agacgcacta cgacgcctgg
     5521 ccgcacaaag tcagtctcgg catcgaatat caacatacct ttaaggcgat taatcaacgt
     5581 aacggagagc gcaacaacgc gtttctcacc attggagtgc actggtaaat gcgtaagttc
     5641 attttcgtat tgttgacact gcttttggtc agcccttttt cctttgcgat gaaaggtatt
     5701 atctggcaac cacaaaaccg tgatagtcag gttaccgata cccagtggca ggggctgatg
     5761 agtcagttac gtttgcaagg cttcgatacc ctggttttgc aatggacccg ttacggcgat
     5821 gcattcaccc agccagaaca gcgcgcgtta ttgtttaagc gtgccgcagc tgcgcaacag
     5881 gctggcctga agctgattgt cgggctgaac gccgatccgg aattttttat acaccagaaa
     5941 cagtcctccg cagcgctgga aagctatctt aatcgcctgc tggctgccga tctccagcaa
     6001 gccagattat ggagcgccgt gcctggcgta acgccggatg gctggtacat cagcgcggaa
     6061 attgacgacc tgaactggcg cagcgaagcc gcccgtcaac ctttgctaac atggttaaac
     6121 aacgcgcagc ggttgattag cgatgtttca gcaaaaccgg tttatatcag tagttttttc
     6181 gccggaaaca tgtcgccaga tggctatcgc caactgctgg aacaagttaa agcaaccggt
     6241 gttaatgtct gggtacagga tggcagcggc gtggataaac tgaccgctga acagcgtgaa
     6301 cgttatctac aggccagcgc cgattgccaa agttccgccc cagcaagcgg cattgtttat
     6361 gaactgtttg tcgctggtaa aggtaaaacg tttacagcga aaccgaaacc ggatgcagag
     6421 atcgcctcgc tgttagcgaa acgttcctct tgcggtaaag acacgctcta tttctctctg
     6481 cgctatttgc ccgtcgcgca gagtatcctt gagtattaat gattaaaggg agcaacttag
     6541 cttgctcctt tccttttcac tttcctaatc aaaatcctgg caagatgaat tgtttagcag
     6601 aatttctgtt ttcattttat tcgtctgacg tgttccaatt atttagcaaa accgcgacaa
     6661 caaagtgatg gtgtaatttg taaaagggaa caaataaatg gcgaggaaat ggctcgccct
     6721 gcaggattcg aacctgtagc ccacgactta gaaactccag gaacgacatt ttaaatcaac
     6781 aaattaccgc gccatctctg cgttcacacg ttccaccacc tcaaaatatg taaaaccttg
     6841 caagccattg cgaggcctta tgtgtctcag ttttgttcct cttttttata ctaaaaaaca
     6901 cagcaattga ggataaacct catgctatta tcgctaatat gcatctaaag gcatggtact
     6961 tgaatagata aaaactccac aaagaaaaaa ataattagcc cataaaacca caatgcaaaa
     7021 cacaaacaga caattaaact aaaaaa
//
LOCUS       DAZUCC010000228         7032 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZUCC010000228 DAZUCC010000000
VERSION     DAZUCC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.229"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            549..1538
                     /gene="araF"
                     /locus_tag="ACHY09_004342"
     CDS             549..1538
                     /gene="araF"
                     /locus_tag="ACHY09_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFS3887225.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1608..3122
                     /gene="araG"
                     /locus_tag="ACHY09_004343"
     CDS             1608..3122
                     /gene="araG"
                     /locus_tag="ACHY09_004343"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFS3887226.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRTHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     ADQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3137..4123
                     /gene="araH"
                     /locus_tag="ACHY09_004344"
     CDS             3137..4123
                     /gene="araH"
                     /locus_tag="ACHY09_004344"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFS3887227.1"
                     /translation="MSSVSTSGSGAPKSSFSLGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4290..5090
                     /gene="otsB"
                     /locus_tag="ACHY09_004345"
     CDS             4290..5090
                     /gene="otsB"
                     /locus_tag="ACHY09_004345"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFS3887228.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5065..6489
                     /gene="otsA"
                     /locus_tag="ACHY09_004346"
     CDS             5065..6489
                     /gene="otsA"
                     /locus_tag="ACHY09_004346"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFS3887229.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6496..6924)
                     /gene="uspC"
                     /locus_tag="ACHY09_004347"
     CDS             complement(6496..6924)
                     /gene="uspC"
                     /locus_tag="ACHY09_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFS3887230.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1762 a         1650 c         1831 g         1789 t
ORIGIN      
        1 gtatcttgtt gatattaatc gattaatttg aattcatgat gaagtgatgg ttttataaaa
       61 cgttttttca tttttgcgag atggctctca ttatacgtgt tctgttaatt aatgagcaca
      121 gtgataatta atggcgaaat gaatgacgca cattttccac atctttgagt tgtggttatt
      181 acaccattgc aaaaactcaa cgccaggtaa tgcggcctat tgactggtta aaaagaagac
      241 atcccgcatg ggtaccaaag acaacaagga cttccaggct aatcttatgg attattctgc
      301 tgtgcactcg ataatttgtc tgacaaattc gcttttcctt atgtcttttc ccgctaaatt
      361 tatgcacgtt ctcactgtaa ttctgcgatg tgatattgct ctcctatgga gaattaattt
      421 ctcgctaaaa ctatgtcaac acagtcactt atcttttagt taaaaggtaa tgctttgttt
      481 tccgattaat ttaacgaatg tcattcgttt ttgccctaca caaaacgaca ctaaagctgg
      541 agagaaccat gcacaaattt actaaagccc tggcagccat tggtctggca gccgttatgt
      601 cacaatccgc tatggcggag aacctgaagc tcggtttcct ggtgaagcaa ccggaagagc
      661 cgtggttcca gaccgaatgg aagtttgccg ataaagccgg gaaagattta gggtttgaag
      721 ttattaagat tgccgtgccg gatggcgaaa aaacattgaa cgctatcgac agcctggctg
      781 ccagtggcgc gaaaggtttc gttatttgta ctccggaccc gaaactcggc tctgccatcg
      841 tggcgaaagc gcgtggctac gatatgaaag tcattgccgt ggatgaccag tttgttaacg
      901 ccaaaggtaa gccaatggat accgttccgc tggtgatgat ggctgcgacc aaaattggcg
      961 aacgtcaggg ccaggaactg tataaagaga tgcagaaacg tggctgggat gtcaaagaaa
     1021 gcgcggtgat ggcgattacc gccaacgaac tggataccgc ccgccgccgt acaacgggtt
     1081 ctatggacgc actgaaagct gcaggattcc cggaaaaaca aatttatcag gtacctacca
     1141 aatctaacga catcccgggg gcatttgacg ctgccaactc aatgctggtt caacatccgg
     1201 aagttaaaca ttggctgatc gtcggtatga acgacagcac cgtgctgggc ggcgtacgcg
     1261 cgacggaagg tcagggcttt aaagcggccg atatcatcgg tatcggtatt aacggtgtgg
     1321 atgcggtgag cgaactgtct aaggcacagg caaccggctt ctacggttcc ctgctgccaa
     1381 gcccggacgt acatggttat aagtccagcg aaatgcttta caactgggta gcaaaagacg
     1441 ttgaaccgcc aaaatttacc gaagttaccg acgtggtgct gatcacgcgt gacaacttta
     1501 aagaagaact ggagaaaaaa ggtttaggcg gtaagtaatt tgccggaaag attcccctct
     1561 gcatgatgca gagggggtgt gaacgaccag tgattcacgg agacgttatg caacagtcta
     1621 ccccgtatct ctcatttcgc ggcatcggta aaacgtttcc cggcgttaag gcgctgacgg
     1681 atattagttt tgactgctat gccggtcagg ttcatgcgtt gatgggtgaa aatggcgcag
     1741 gaaaatcaac tctcttaaaa atcctcagcg gaaactatgc gccaaccacg ggttctgtag
     1801 tgattaatgg gcaggaaatg tccttttccg acacgaccgc agcacttaac gcgggggtgg
     1861 cgattattta ccaggagctg catctcgtgc cggaaatgac cgtcgcggaa aacatctatc
     1921 tcggccagct gccgcataaa ggcggcattg tcaatcgctc attgctgaat tatgaggcgg
     1981 gtttacaact taaacatctt ggtatggata ttgacccgga cacgccgctg aaatatctct
     2041 ccattggtca gtggcagatg gttgaaattg ccaaagccct ggcgcgtaat gccaaaatta
     2101 tcgcctttga tgagccaacc agctccctct ctgcccgtga aatcgacaat cttttccgcg
     2161 ttattcgtga actgcgaaaa gaggggcggg tgatcttata cgtttctcac cgtatggaag
     2221 aaatatttgc cctcagcgat gccatcactg tctttaaaga tggacgttat gtcaaaacct
     2281 ttaccgatat gcagcaggtt gaccacgacg ccctggtgca ggcgatggtc gggcgcgaca
     2341 tcggcgatat ctacggttgg caaccgcgta gttatggcga ggagcggctg cgtcttgatg
     2401 ctgtgaaagc accaggcgtg cgtacgccaa taagtctggc ggttcgcagt ggagaaattg
     2461 tcggtctgtt tggtctggtc ggggcagggc gtagcgaatt aatgaaaggc atgtttggcg
     2521 gtacgcaaat caccgccggt caggtttata tcgaccaaca gccgatcgat attcgtaaac
     2581 cgagccacgc cattgccgca ggcatgatgc tctgcccgga agatcgcaaa gcggaaggca
     2641 ttattcccgt gcactccgtt cgcgacaata tcaacatcag tgccagacgt acacatgtgc
     2701 tcggcggttg tgtaatcaac aacggttggg aagaaaacaa tgccgatcac cacattcgtt
     2761 cgctcaacat caaaacgccg ggcgctgatc aactgatcat gaatctctca ggcggaaatc
     2821 agcaaaaagc cattctgggc cgctggttat cggaagagat gaaggtcatt ttgctggatg
     2881 aacctacgcg cggcattgat gttggtgcta agcacgaaat atataacgta atttatgcgc
     2941 tggcggcgca gggtgtggcg gtgctgtttg cctccagcga cttacctgaa gtcctcggcg
     3001 ttgccgaccg gattgtggtg atgcgggaag gcgaaatcgc cggtgaattg ttacacgagc
     3061 aggcagatga gcgtcaggca ctgagccttg cgatgcctaa agtcagccag gcagttgcct
     3121 gagtaaggag agtatgatgt cttctgtttc tacatcgggt tctggcgcac ctaagtcgtc
     3181 attcagctta gggcgtatct gggatcagta cggcatgctg gtggtgtttg cggtcctttt
     3241 tatcgcctgt gccatttttg tcccgaactt tgccaccttt attaatatga aagggttggg
     3301 tctggcaatt tccatgtcgg ggatggtggc ttgtggcatg ttgttctgcc ttgcttccgg
     3361 tgactttgac ctttctgtcg cctccgtaat tgcctgtgcg ggtgtcacca cggcggtggt
     3421 tattaacctg actgaaagcc tgtggattgg cgtggcagcg ggtttgttgc tgggcgttct
     3481 ctgtggcctg gtcaatggct ttgttattgc caaactgaaa ataaatgccc tgatcacgac
     3541 actggcaacg atgcagattg ttcgaggtct ggcgtacatc atttcagacg gtaaagcggt
     3601 cggtatcgaa gatgaaagct tctttgccct tggttacgcc aactggttcg gtctgcctgc
     3661 gccaatctgg ctcaccgtcg cgtgtctgat catctttggc ttgctgctga ataaaaccac
     3721 ctttgggcgt aataccctgg cgattggcgg gaacgaagag gccgcgcgtc tggcgggtgt
     3781 accggttgtt cgcaccaaaa ttattatctt tgttctctct ggcctggtat cagcgatagc
     3841 cggaattatt ctggcttcac gtatgaccag tgggcagcca atgacgtcga ttggctatga
     3901 gctgattgtg atctccgcct gcgttttagg tggcgtttct ctgaaaggcg gcatcggaaa
     3961 aatctcatat gtggtggcgg gtatcttaat tttaggcacc gtggaaaacg ccatgaacct
     4021 gcttaatatt tctcctttcg cacagtacgt agttcgcggc ttaatcctgc tggcagcggt
     4081 gattttcgac cgttacaagc aaaaagcgaa acgcactgtc tgatgctttt ttccacaaca
     4141 atttagcgtt ttttcccacc acagccagcc gccacaacgg ttggctgttc ttcgttgcaa
     4201 atggcgaccc ccgtcacact gtctatactt acatgtctgt aaagcgcgtt ctgcgcaaca
     4261 caataagaaa agagaaggag gagaaccggg tgacagaacc gttaaccgaa acccctgaac
     4321 tatccgcgaa atatgcctgg ttttttgatc tcgatggaac gctggcggaa atcaaaccgc
     4381 atcccgatca ggtcgtcgtg cctgacacta ttctgcaagg acttcagcta ctggcaaccg
     4441 caagtgatgg tgcattggca ttgatttcag ggcgctcaat ggtggagctt gacgcactgg
     4501 caaaacctta tcgcttcccg ttagcgggcg tgcatggggc ggagcgccgt gacatcaatg
     4561 gtaaaacgca tatcgttcat ctgccggatg cgattgcgcg tgatattagc gtgcaactgc
     4621 atacggctat cgcccagttt cccggtgcgg agctggagac gaaagggatg gcctttgcgc
     4681 tgcattatcg tcaggctccg cagcatgaag acgcattaat gacattagcg caacgtatta
     4741 ctcagatctg gccacaaatg gcgttacagc agggaaagtg tgttgtcgag atcaaaccgc
     4801 gaggcaccag taaaggtgag gcgattgctg cctttatgca ggaagctccc tttatcgggc
     4861 gaacgcctgt atttctgggc gatgatttaa ccgatgaatc tggcttcgca gtcgttaacc
     4921 ggctgggcgg aatgtcagta aaaattggca caggcgcaac tcaggcatca tggcgactgg
     4981 cgggcgtgcc ggatgtctgg agctggcttg aaatgataac caccgcatta caacaaaaaa
     5041 gagaaaataa caggagtgat gactatgagt cgtttagtcg tagtatctaa ccggattgca
     5101 ccaccagacg agcacgccgc cagtgccggt ggccttgccg ttggcatact gggggcactg
     5161 aaagccgcag gtggactgtg gtttggctgg agcggtgaaa cagggaatga ggatcagccg
     5221 ctaaaaaagg tgaaaaaggg taacattacg tgggcctctt ttaacctcag cgaacaggac
     5281 cttgacgaat actacaacaa attctccaat gccgttctct ggcccgcttt tcattatcgg
     5341 ctcgatctgg tgcaatttca gcgtcctgcc tgggatggct atctacgcgt aaatgcgttg
     5401 ctggcagata aattactgcc gctgttgcaa gacgatgaca ttatctggat ccacgattat
     5461 cacctgttgc cctttgcgca tgaattacgc aaacggggag tgaataatcg cattggtttc
     5521 tttctgcata ttcctttccc gacgccggaa atcttcaacg cgctgccgac atatgacacc
     5581 ttgcttgaac agctttgtga ttatgatttg ctgggtttcc agacggagaa cgatcgtctg
     5641 gcgttcctgg attgcctttc taacctgacc cgcgtcacga cacgtagcgc aaaaagccat
     5701 acggcctgcg gcaaagcgtt tcgaacggaa gtttacccga tcggcattga accgaaagaa
     5761 atagcgaaac aggctgccgg accactgccg ccaaaactgg cgcaacttaa agcggaactg
     5821 aaaaacgtac aaaatatctt ttctgtcgaa cggctggatt attccaaagg tttgccggag
     5881 cgttttctcg cctatgaagc gttgctggaa aaatatccgc agcatcatgg taaaattcgt
     5941 tatacccaga ttgcaccaac gtcgcgtggt gatgtgcaag cctatcagga tattcgtcat
     6001 cagctcgaaa atgaagctgg acgaattaat ggtaaatacg ggcaattagg ctggacgccg
     6061 ctttattatt tgaatcagca ttttgaccgt aaattactga tgaaaatatt ccgctactct
     6121 gacgtgggct tagtgacgcc actgcgtgac gggatgaacc tggtggcaaa agagtatgtt
     6181 gccgctcagg acccagccaa cccgggcgta cttgttcttt cgcaatttgc gggagcagca
     6241 aacgagttaa cgtcggcgtt aattgttaat ccctacgatc gtgacgaagt tgcagctgcg
     6301 ctggatcgtg cattgactat gtcgctggcg gaacgtattt cccgtcatgc agaaatgctg
     6361 gacgttatcg tgaaaaacga tattaaccac tggcaggagt gcttcattag cgacctaaag
     6421 cagatagttc cgcgaagcgc ggaaagccag cagcgcgata aagttgctac ctttcctaag
     6481 cttgcgtagg agcggttaat ctcccgtaag tggaactaac agcacatcga cctcacttga
     6541 ggcaatcact cttttcgccg agcaggatgc tcgcgaaaag aaactgtgat tgtgattacc
     6601 gcaaatcacc aaatcgaaat gatatttgcg acaaacttcc agaatatgtt cgcttaactc
     6661 accgtaggca ataaacgtct tgtcaacggg ataccccgca tcctgaatta acttatcaag
     6721 aaagctttgc gtttcttcct gcatcacgct gcgtaaatct tcaagcatcg gcgcagctaa
     6781 ttgattgtac atttccgggt cggaagcgag agtaattaaa ctgatgtgtc cctttactgg
     6841 cctggcgata gataccgctt ttgccagcag ttgctgactt tccggtgtaa ccgcaacagc
     6901 gacaagaata ttgctatagc tcataacctg ttccttattc tgtgaacttc aggtgacatt
     6961 aaagctttac tcctaatgga gaaacgacgc aatcccaact cagtcaaaca ggtaaatatt
     7021 agcgtgatta tt
//
LOCUS       DAZUCC010000229         7009 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZUCC010000229 DAZUCC010000000
VERSION     DAZUCC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.230"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(24..2807)
                     /locus_tag="ACHY09_004348"
     CDS             complement(24..2807)
                     /locus_tag="ACHY09_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS3887231.1"
                     /translation="MRNLCFLLMLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPSNKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNAQDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLLPLITLYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLETRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2864..5236)
                     /locus_tag="ACHY09_004349"
     CDS             complement(2864..5236)
                     /locus_tag="ACHY09_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFS3887232.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDFTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDVNPQ
                     IVFSYIKSKGNLSLNNGYEESNTDDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYINEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5274..6947)
                     /locus_tag="ACHY09_004350"
     CDS             complement(5274..6947)
                     /locus_tag="ACHY09_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS3887233.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     LITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRPLNVE
                     KQRSEATFRTNLVQHNKQAELIALSNAEYLQRQELSDNFHTIKENWHRLMNRQRWLDY
                     WQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDELAEL
                     AAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSPGKWL
                     LLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICKALPL
                     SVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDE
                     TTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVI"
BASE COUNT         1813 a         1603 c         1578 g         2015 t
ORIGIN      
        1 acgatgtgtt gacggggctg ttattatttt ggcaataata ctccggtata ggtattcacc
       61 ggatgagaaa gatattgttt aacggcagtg ttaacattct ctaccgtcat ttgtttcaat
      121 aattgctcct gctcagtcca tgctgcagga tcgtcatatt gaataagact atttacaatg
      181 gtgttcgcta attgttgaac gctacgctgt tggatatcga ggctgcgctg aacgttttgc
      241 tggtattcgt tcagttcttg ctcactgatc cctttagcca gacgcttaac catcacttca
      301 ttcgctaacg ttaacagttc atcatgtcgt tccggttgac aagtaaaagc cagcaaatga
      361 ctgatatctt tggcctgagg atcaaccgag aggcgagaag aaacgctgta tgccccagag
      421 gcctgttcac gaatattaac gcgtagatct tttgccagtg cgacgttaaa agcatcgagc
      481 gccatacgcg tctccagatt aaccggtgtc cgggaatcat aacgcttcca ctgtgaaacc
      541 tgtgccacag gttcattttg ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt
      601 aatggtttac ctgcggctaa tggcgaatca gagtgtttga ttgatcctaa gtaaagcgta
      661 attaacggca ggagtttgtc ttctgcgaca ttaccgacaa tgacaaacgt gatatccgct
      721 ggagatgaaa acaactggcg atcggcagcc agcgcatctg cggcagtaaa ctgtgcaatc
      781 tgattttctt gcagtaattt cgtgcggtca tcagcatagc gcgtctcata catctgctgg
      841 gcgaatttct ccgctggacg ctggtcgagc gttttcaacg cctgaatttg agcattttgt
      901 agcgatgccc aaatattatc gttaatcgtg ctgtgggtga ttcgctggtt aatcaactgg
      961 aaaccaggtt cagggttatt agtccgcgcg ctaacagaga gcaatgtatt catgccactg
     1021 actttactgc tcatggttac cgagttttcc gcactccagc gtttcaggct ggaagaggag
     1081 agttcaccaa cgccgcttcc gcttactgct ttatttgcca gcgcgataag tgatttttgc
     1141 tgcgcaggga aacttaaatc gcctttatta gatacggcag taatttgtag cttttgctct
     1201 tcacccgcgg attttgccag aataaccctg gcaccattgg aaagtgttaa tgatgtcaga
     1261 ttctcagcca gcgtttcttt gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac
     1321 gataaatttc tgcctgggaa gatgtaagcc gccagctttt tgttggcgta ctctttttcc
     1381 agcgccagga tagccgcagg agacaatgct tttttggcgg caacttcatt gtttaccatt
     1441 tgctcccaaa atgcgtcctg gttctttctt aactgctgcc atttttccgc cagactttgc
     1501 acggtaattt gctgccacag acgtttcgaa agttgatatg tctcttccgg cgacaagaac
     1561 ggcgtattat ttaatgagct ggatgccagg cgactggtca gcatacgtaa atcacgctca
     1621 gcttgctgat caaccgcatt tttcagccag gtaaggcggg tagatttaac atcatcgagt
     1681 tcttcagcag aaaagccatg ctgatcaatg gttgccaact ctgccattaa tgcattcgca
     1741 gcatcctgca tattatcgtc ttgtgcattt acacggaaaa acaaagactg ataatcgggt
     1801 gcaattttga cgctgcgcgc agtgccgcca gaaatagtct tcaactcgcc cgactgtatg
     1861 cgttcttgca gacgttggtt gaacagctga accaacatgc tccattcagc ttgttcgata
     1921 aagctttgct catcgttcac ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc
     1981 acccggtttt ctttatcatt gatgatatta aagcgcaggt ggttttcggc ttttgtcggc
     2041 cagacgcgat tttcagctgc tttgttagac ggaagcttac ttaaattatc cttaatcagc
     2101 gccagcgctt ctttactgtc aatatcgccg accacgataa aggtcatatt atttggttga
     2161 taccagcgtt gataaaattg gcgcaattgt gccggtgtga ccgtagcgac agtatccatc
     2221 aggccgatag gttcacggtc taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc
     2281 tgagaggtgc gccatttcgc atcctgatgg gcacgccatt cctcagtaat tacgccgcgt
     2341 tcagcgtcta cttcgagttt ttcaaaggtt gcggcattac tccattcact gaagattgcc
     2401 atcacttgtt gcagattttg tttctgcgta gtcggcaaac tcacctgata cactgtttcg
     2461 tcatagctgg tataggcatt aacatcgcga ccaaaacgca ggcccattga ctcaaatgtt
     2521 tcgatgactt tattacccgg ccatgttttt gtgccgttaa acatcatatg ttctacaaaa
     2581 tgagccacgc cgcgctcatt gtcctcttcc tgcaatgaac cggtatgaat ttgcagccat
     2641 aaatttacct gatcctttgg ttgagcatgc ggataaatca tatatcgcaa gccattgtcc
     2701 agttgcccgg taattaactt ttcatcctgc ggtaaggcgg cggcaatcag tcgcccgggg
     2761 agcaacagag ttgccactaa catcagtaag aaacagaggt ttctcataat tatctccatg
     2821 cgaaaaccgg gcaaatttac ccggtaaagt aaaatccgaa ctattaaaat ttcatgctga
     2881 catcaagcca gaaagtacgg ccactggcgt aggtcgccac gccggtattc gttgtatcaa
     2941 ccgcggtttt gctatccagt acattgagaa tatcggcact gatcgtcagg ttttgttgtt
     3001 tcagaaattg tggcgtccag gacaagcggg tgtcccaggt caggctgcta tccagtttct
     3061 cgtcaacata ctgcttgtaa tctgaatatt cgttgatgta ttgcgcattc gtcttaccca
     3121 ggataatgcg agctttacgc gcttcttgcc aggtcagcgt attcgcccac accaacccgc
     3181 tcggttgatg cgtgaaatcc atgtttaagg agatctttaa tggattatta aaatccgcca
     3241 ctggaacgct atcgtaagag accagattac cgttataaac cacccggtta tcatcggtat
     3301 tgctttcctc ataaccattg tttaacgaca agttgccctt gctcttgata tagctaaaga
     3361 caatttgcgg gttaacatct acctggctga tatgcagggg ttcggccagt tcaaaactga
     3421 ggttgaacga atgcgttttg gttttgccgt cgttgttata ttcagtaatg gtggttttag
     3481 tcgcgctgtc ggtacgactg cttttgctga tttgatcatg cgcttcacgg taaacatagt
     3541 ttgcgcgcgc aataacgttc ttaccgattt tctgctgcaa ccccattgcc agttcatcgt
     3601 tataaggcgt tttcaaatcc tgataacgcg tcagggtttt attgcctgat accgattccg
     3661 tccagctatt gcggatatca cgtaatccca tatcaagaat attcccgcca tagtaacggt
     3721 tataacctgc ggtaatcatt gaggtttgat cagcaaaaat atcccattcc gtcataaagc
     3781 gcggggagat attgtggttt gacagatagt tgtcatagtc ataccgcaca ccgggcatta
     3841 acgacacatt ccgccagcta atgcgatccg ccatatacag cgtgtagttg tcaattccca
     3901 gactgccttt acctttatgg taaatggtat ggttagtttt ctttcctgca gcattaatca
     3961 cataggattc agactggtta tggcgttcag tccatgcatc ggaatagatg tattccgcgc
     4021 cgaagtaggg ttgatgcgaa acatcaccca cggcgaattt ttgccagtcc aggcgtgttt
     4081 tgaaggtgta attatctaca gcctgggaaa tgtgtcctaa tccaccacgg gtgcaacgac
     4141 ctgaaatatc accatatgta catgaaagtt cggtgtacca gatgtcatga tcgtgacggg
     4201 tataatcact aatatgatcc caaccaacgg tagtacgtag tttggcccat gcgagctgcg
     4261 tatccatatc ccatgccaga ccgtaggatt tattacccat ttcgcgatca gactgcggga
     4321 aggtgctggt attataatca cgactggagc cggtatattt taaggtaaaa tcgtgggtaa
     4381 agcggtcgct ggcaaaccaa gtaaatttgc tcaatgcggt atcgataacg tttttatact
     4441 gtgcccgacc ggcgacaatg ccgtcattcg aaacataatc cgcgcgggtg atatcagact
     4501 ggcggcgcga taaaccggcg gtaacgccaa agttatcagc gagttcctga ttaaacgaca
     4561 aggtataaaa gttcttttta aaatctggag agtaataagt acttcctgaa gaaccttgat
     4621 taaatgcgct cttgttattc tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc
     4681 gataacccag tttcacgctg ctatcatcag cgttgaagcg tttgatcttt gcatcaatta
     4741 ccccgccatt gaagcgaccg aattcaaccg gcacaaaact gtcataaagc gtcacattgt
     4801 ccagtaagct gacatcaaga taatagccct gtgacatccc gctaatattg gttgcactac
     4861 tggcatcgga ctcattcgct gggttcaggt tattagttgc actaatacca tcaatcaaat
     4921 aggcattctg gtagggcgac gcaccgtgaa tagagatttt ctccgggcga atatctccct
     4981 ggttcaatga ggtactttgc gttgaatcca tgcgcaccgc agggttggtt ctcagcagat
     5041 cgctgatatt gccattcccg gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt
     5101 tgccgtttac gggtacaggg acaggagcat agaccgtcat ctcttcaaaa acatgcatgt
     5161 acatgttttt gtcatcgacg gcctgcgcta catcagcgcc acataaaatg acgccaggaa
     5221 taagaactcg cttcatttat ttatatctcc gtacagcagg gcttttattg tttttatata
     5281 accgcgctaa tgtcacaaat atcatcagcc aggttccaga caccgggttg atgtgtaacc
     5341 ataatgacgc cgcttgtggg tagtttttca cgcactaaac gcagtaagcg gatagcctct
     5401 tgttcctcaa gatgagaggt agtttcgtca agaaatatcc atttcggacg tcgtaaaatt
     5461 aatcgtgcca gggcgatacg ttgtttttcg ccgctggaaa gaatatcgcc ccagcgatcg
     5521 tggtcatgaa tacgcgcagc caatttcccc agaccaacct gatgcagtac ttcgctcaac
     5581 gatttatcgt ctacggacag gggaagtgct ttacaaatta tctctttcag taagccggtt
     5641 ttgattaacg gtgtttgtga cacataccaa ctgtcagcag gagaagaaat atcaccttta
     5701 aaccacggcc agcagtggga taatgtttta agcagtgtgg tttttcccgc accagagtaa
     5761 cctttcagta atagccattt gcctggcgaa acatgaaagt tcaggttctc taatatgatc
     5821 ttattatcag gagtacgaat actcgcgtca gccacttgta ccgcatgttg gcaattttta
     5881 ggcttattcg tagggcgctg ttcggtgagt tgatggaact catacaaacg atcgataacc
     5941 gcagccagtt cagcaagttc gtcatattta taaataaacc agcttaaatt gttcgatacc
     6001 agcataaatg cctggcgcga tttcatcagt ccgcccagat tgatctgtcc gctaataaac
     6061 tgcggcaata acagaaagta gggaagaacg ctaagcgagc gcgaataaat attctgccag
     6121 taatcaagcc accgctgacg attcattaaa cgatgccagt tctctttaat ggtatgaaaa
     6181 ttgtcgctta actcctggcg ttgtagatac tcagcattgc ttagcgcaat cagttctgcc
     6241 tgcttattat gctgcacaag attagttcga aatgtcgctt cgctacgttg tttttccaca
     6301 ttaagcggac gaatacgttt accaacctta tgggtaaata aagttccacc gatcacaatg
     6361 agcacaacgg tatagaccat atacccctgg atattccatt ctgttccacc aacagtgaat
     6421 gagagcgtac ccgcgctttg ccaaagaata acggtaaagg tgatcaactg gatgaagcca
     6481 aatgaaaggc tgagcgtttt actgatcagt aagagaatgt cctcagcgat acgttggtca
     6541 gggttatctg tatttttatg ctcaccgtag atctgcgtga agtagtagtt tttatctgca
     6601 aaccagcgat taaggtagta atctgttaac cattcgcgcc agcgaatagt cagtagttta
     6661 attagccatg ttttgtttac ggatattaat acaaagatcc ccagcagggc agggaaccaa
     6721 aggacaagct gccaaagctt atcggtttct ttctggctta gcgcattgaa aaaatcatta
     6781 ttccagtcgt ttaaccagac ctgaatttta accacgccaa ggatcatggc gaggataata
     6841 atgattaaca acactgaagt tttattattc ttacgcagcc aaaatggctt cagcagacag
     6901 agatactttg ctatcaacat acaaaacgta atgggaatgg ttatcattag cgaaaattga
     6961 ttgtgccatc caatgattat ctatggcaat gatttactga tgaaattta
//
LOCUS       DAZUCC010000230         6932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZUCC010000230 DAZUCC010000000
VERSION     DAZUCC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.231"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..1515
                     /gene="ulaA"
                     /locus_tag="ACHY09_004351"
     CDS             118..1515
                     /gene="ulaA"
                     /locus_tag="ACHY09_004351"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS3887234.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKFAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1531..1836
                     /gene="ulaB"
                     /locus_tag="ACHY09_004352"
     CDS             1531..1836
                     /gene="ulaB"
                     /locus_tag="ACHY09_004352"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFS3887235.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1846..2310
                     /gene="ulaC"
                     /locus_tag="ACHY09_004353"
     CDS             1846..2310
                     /gene="ulaC"
                     /locus_tag="ACHY09_004353"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFS3887236.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2324..2974
                     /gene="ulaD"
                     /locus_tag="ACHY09_004354"
     CDS             2324..2974
                     /gene="ulaD"
                     /locus_tag="ACHY09_004354"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS3887237.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2984..3838
                     /gene="ulaE"
                     /locus_tag="ACHY09_004355"
     CDS             2984..3838
                     /gene="ulaE"
                     /locus_tag="ACHY09_004355"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFS3887238.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEISVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3838..4524
                     /gene="ulaF"
                     /locus_tag="ACHY09_004356"
     CDS             3838..4524
                     /gene="ulaF"
                     /locus_tag="ACHY09_004356"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFS3887239.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4653..4928)
                     /gene="yjfY"
                     /locus_tag="ACHY09_004357"
     CDS             complement(4653..4928)
                     /gene="yjfY"
                     /locus_tag="ACHY09_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFS3887240.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5256..5651
                     /gene="rpsF"
                     /locus_tag="ACHY09_004358"
     CDS             5256..5651
                     /gene="rpsF"
                     /locus_tag="ACHY09_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS3887241.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5658..5972
                     /gene="priB"
                     /locus_tag="ACHY09_004359"
     CDS             5658..5972
                     /gene="priB"
                     /locus_tag="ACHY09_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFS3887242.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5977..6204
                     /gene="rpsR"
                     /locus_tag="ACHY09_004360"
     CDS             5977..6204
                     /gene="rpsR"
                     /locus_tag="ACHY09_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS3887243.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6246..6695
                     /gene="rplI"
                     /locus_tag="ACHY09_004361"
     CDS             6246..6695
                     /gene="rplI"
                     /locus_tag="ACHY09_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS3887244.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         1643 a         1718 c         1914 g         1657 t
ORIGIN      
        1 ggtcgcgtca catttaatca taaataatct tgttgtgatt acttttgaaa attagagtga
       61 gtgcacaaca ttccgggtgt gtggaatacc cggttacctc ttcttcagga gatcgttatg
      121 gagatcctct acaacatctt taccgtgttt tttaaccagg tcatgaccaa tgccccgttg
      181 ttgctgggta ttgtgacctg tctgggctac atcctactgc gcaaaagtgt cagcgttatt
      241 attaaaggca cgattaaaac cataattggt ttcatgttgt tgcaggcagg atccggcatc
      301 ctcaccagca ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg cattaacggc
      361 gcaatttccg atacttacgc gtcgatgatg gcaaccatcg accgcatggg cgatgcctat
      421 agctgggtag gttacgcggt gttgttagcg ctggcactga acatctgtta cgtgctgctg
      481 cgtcgcatta ccggtattcg taccatcatg ctgactggtc acatcatgtt ccagcaggcg
      541 gggctgattg ccgtcacgct gttcatcttt ggctactcca tgtggacgac cattatctgc
      601 accgcgattc tggtttcgct ctactggggc attacctcca acatgatgta caagccgact
      661 caggaagtga cggacggctg cggtttctcc atcggtcacc agcaacagtt tgcatcatgg
      721 attgcctata aatttgcgcc gttcctcggc aaaaaagagg aaagcgttga agacctcaaa
      781 ctgccaggct ggctgaatat tttccacgac aacatcgtct ccacggcgat tgtcatgacc
      841 atcttctttg gtgccattct gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc
      901 aaagtgaact ggacggtgta catcctgcaa actggtttct ccttcgcggt ggcgatcttc
      961 atcatcactc agggtgtgcg catgtttgtg gcggaactct ctgaagcgtt taacggtatc
     1021 tcccagcgtc tgatccctgg tgcggttctg gcgattgact gtgcggctat ttatagcttc
     1081 gcgccgaacg ccgtggtctg gggctttatg tggggcacca tcggtcagct gattgcggtt
     1141 ggcatcctgg tcgcctgcgg ttcctcgatc ctgattatcc caggctttat cccgatgttc
     1201 ttctccaacg ccactattgg cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg
     1261 aagatttgtc tggtgatggg gatgatcgaa atctttggct gcgtctgggc ggtgaaactt
     1321 accggtatga gtgcctggat gggtatggcg gactggtcga ttctggcacc gccgatgatg
     1381 cagggcttct tctccatcgg tatcgccttt atggctgtca tcattgtaat tgcactggct
     1441 tatatgttct tcgctggccg cgcactgcgc gcagaagaag atgcagaaaa acaactggca
     1501 gaacagtctg cttaataagg agttttgatt atgaccgtac gtattctggc tgtgtgtggc
     1561 aacggacaag gcagttccat gatcatgaaa atgaaagtgg accagttttt aacccagtcg
     1621 aacattgacc acacggtaaa cagctgcgcg gttggcgaat acaaaagcga gttgagcggc
     1681 gcggatatca tcatcgcttc tacccatatt gcgggcgaaa tcactgtgac cggcaacaaa
     1741 tatgtggttg gcgtgcgcaa tatgctctct cctgccgact ttggcccgaa attgctggaa
     1801 gtgatcaaag agcattttcc gcaggacgtg aagtaaggac gcgccatgaa attacgtgat
     1861 tcgctggcgg agaataaatc catccgcctg caggctgaag ctgagacatg gcaggacgca
     1921 gtaaaaatcg gcgttgacct gctggtggcg gcggatgttg tcgagccgcg ttactaccag
     1981 gcgattctgg atgcggttga acagcacggt ccgtactttg tgctcgcacc cggcctggca
     2041 atgccgcacg gtcgcccgga agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg
     2101 aaaaagccgc tggagttcaa ccacgaagat aacgatccgg tggatatcct catcaccatg
     2161 gcggcggtcg atgccaacac tcaccaggaa gtgggcatca tgcagatcgt caatctgttt
     2221 gaagatgaag agaatttcga ccgtttacgt gcctgccgta ccgagcagga agtactggat
     2281 ctcattgacc gcaccaacgc ggcagcttaa gaaggaattg aaaatgtcat taccgatgtt
     2341 gcaggtcgcg ctggacaacc agactatgga tagcgcctat gaaaccactc gcctgattgc
     2401 cgaagaagtc gacattatcg aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc
     2461 ggttcgcgat ctgaaagcgc tttacccgca caaaatcgta ctggcagacg ccaaaattgc
     2521 cgatgcgggc aaaatccttt ctcgtatgtg cttcgaagct aacgctgact gggtgaccgt
     2581 gatttgctgt gcggatatca acacagctaa aggtgcgctg gacgtggcaa aagagtttaa
     2641 cggcgacgtg cagatcgaac tgaccggtta ctggacctgg gaacaggcgc aacagtggcg
     2701 cgacgcaggc attgggcagg tggtttatca ccgcagccgt gacgcacagg ccgcaggtgt
     2761 ggcgtggggc gaagcggaca ttaccgcgat caaacgtctt tccgatatgg gcttcaaagt
     2821 caccgtcacc ggtggtctgg cgctggaaga tctgccgttg tttaaaggca ttccgattca
     2881 tgtgtttatc gctggtcgca gcattcgtga tgccgcttct ccggtggagg ccgcacgtca
     2941 gttcaaacgt tccatcgctg aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat
     3001 cccgcttggc atctatgaaa aagcgctccc cgccggggag tgctggctgg aacggctgca
     3061 actggcaaaa acgttaggct tcgattttgt cgagatttcg gtagatgaaa cggacgatcg
     3121 cctgtcgcgc ctcgactgga gccgcgagca gcgtctggcg ctggtgaacg cgattgttga
     3181 aaccggcgtg cgcgtgccgt ccatgtgcct ttctgctcat cgtcgtttcc cgctgggcag
     3241 cgaagatgac gcagtgcggg cgcaggggct ggagattatg cgcaaagcta tccagttcgc
     3301 ccaggatgtc ggtattcgcg tgatccaact ggcgggctac gacgtttact atcaggaagc
     3361 caataacgaa acgcgtcgtc gtttccgtga cggcctgaaa gagagcgttg agatggcaag
     3421 ccgcgcgcag gtgacgctgg cgatggagat catggattac ccgttgatga actccatcag
     3481 caaggcgctg ggttacgcgc actatcttaa caacccgtgg ttccagctct acccggatat
     3541 cggcaacttg tcggcgtggg acaacgacgt gcagatggag ttgcaggccg ggatcgggca
     3601 tatcgtcgcg gtgcatgtga aagacaccaa accaggcgtc ttcaaaaacg tcccgtttgg
     3661 cgaaggtgta gtggatttcg aacgttgctt cgaaacgctc aaacagagtg gttattgcgg
     3721 gccgtacctg attgagatgt ggagcgaaac ggcggaagac ccggcggcag aagtggcgaa
     3781 agcgcgtgat tgggtgaaag cgcgcatggc caaagccggc atggtggagg cggcataatg
     3841 caaaagctaa aacagcaggt atttgaagcc aatatggatc tgccgcgcta cgggctggtg
     3901 acctttacct ggggcaacgt cagcgccatt gaccgcgaac gcgggctggt ggtgatcaag
     3961 cccagcggcg ttgcctacga aaccatgaaa gcagacgata tggtggtggt tgatatgagc
     4021 ggcaaggtgg tggaagggga gtatcgtccg tcttccgaca ccgcgacgca tcttgaactc
     4081 taccgccgtt atccgtcgct tggcggcatt gtccataccc attccacgca tgccaccgca
     4141 tgggcgcagg cggggctggc tatcccggca ttaggcacca cgcacgctga ctacttcttt
     4201 ggcgatatcc cctgtacgcg cgggttaagc gaagaagaag tgcaggggga atacgagcta
     4261 aacactggca aagtgattat cgaaacgctg ggtgacgccg agccgctgca tacgccggga
     4321 attgtggtgt atcagcacgg gccattcgcc tgggggaaag atgctcacga tgcggtgcat
     4381 aacgcggtgg tgatggaaga agtggcgaaa atggcgtgga ttgcccgcgg cattaacccg
     4441 caactgaatc acatcgacag ctatctcatg aataaacact tcatgcgtaa gcacggtccc
     4501 aacgcctatt acgggcagaa gtaaaacacg cgctgcggag atttccttct tcgggagata
     4561 actggtctaa gtccgcagcc gtttttcaaa aaaagccccc tgaaaagggg gcaaagcaaa
     4621 ctatggcaat gtttcgttgg ttatacctgg tgctagcgat aaatatccgc gctggcgtgc
     4681 atattgccgt tactccccgg ttcccgcatc agaatcacgt ggtagtacgt tgcgccctgc
     4741 gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa attatgattg
     4801 atataaatca cgcctaagct ttgcacatcg tccatatttc tggcctggtg gttattaatt
     4861 tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc taaaacacgg
     4921 ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctcttgt ggtgatcttc
     4981 tgatttaatt ttaatcaatg ataaagaagt ggatggtgac catttctgat gcagttgttc
     5041 aaaaaaacac catgatgaag tgtgatgaac ttcaaatcag cgtgttagta cgatttggtg
     5101 aaggatgaga tttatttgtc ggctggcctt gagtttagcg gggtatgtca gtactataac
     5161 gcgtcatttt tcagccgacc tttaacacgt tccttgcctc cccgggattc ggctgaccca
     5221 gacaggaggc tgaataatcc gtaaggagca attcgatgcg tcattacgaa atcgttttta
     5281 tggtccatcc tgaccagagc gaacaggttc cgggcatgat cgagcgctac actgctgcca
     5341 tcactggtgc agaaggcaag atccaccgtc tggaagactg gggccgccgt cagctggctt
     5401 acccgatcaa caaactgcac aaagcacact acgttctgat gaacgttgaa gctccgcagg
     5461 aagtgatcga tgagctggaa actaccttcc gcttcaacga tgccgttatc cgcagcatgg
     5521 ttatgcgtac caagcacgct gttaccgaag catctccgat ggttaaagcg aaagacgagc
     5581 gccgtgagcg tcgcgatgat ttcgcaaacg aaaccgctga tgatgctgaa gctggggatt
     5641 ctgaagagta atttctgatg accaaccgtc tggtgttgtc cggcaccgtg tgcaggactc
     5701 cccttcgaaa ggtcagtcca tcaggaattc ctcactgcca gttcgtgctt gagcatcgtt
     5761 ctgtgcagga ggaagccggc tttcaccggc aggcatggtg ccaaatgccc gttattgtta
     5821 gcggacacga aaaccaggcc attactcaca gtataacggt cggcagtcgc ataaccgttc
     5881 aggggttcat ttcatgccat aaggcaaaga acggactgag caaaatggtt ttgcatgccg
     5941 agcagattga attgatagat tctggagact agccatatgg cacgttattt ccgtcgtcgc
     6001 aagttctgcc gtttcaccgc ggaaggcgtt caagagatcg actataaaga tatcgctacg
     6061 ctgaaaaact acatcaccga aagcggtaag attgtcccaa gccgtatcac cggtacccgt
     6121 gcaaaatacc agcgtcagct ggctcgcgct atcaaacgcg ctcgctacct gtccctgctg
     6181 ccgtacactg atcgccatca gtaatcggtc acggtccatt aatacgactt tgagaggata
     6241 aggtaatgca agttattctg cttgataaag tagcaaacct gggtagcctg ggtgatcagg
     6301 taaacgttaa agcgggctat gctcgtaact tcctggtacc gcagggtaaa gctgttccag
     6361 ctaccaagaa aaacattgaa ttcttcgaag cacgtcgcgc tgaactggaa gctaaactgg
     6421 ctgaagttct ggcagcagct aatgctcgcg ctgagaaaat caatgcactg gaaactgtta
     6481 ccatcgcgtc taaagctggc gacgaaggta aactgttcgg ttccatcggt actcgcgaca
     6541 tcgctgacgc tgtaactgca gctggcgttg aagtggctaa gagcgaagtt cgtctgccga
     6601 acggcgttct gcgtaccact ggcgaacacg aagtgagctt ccaggttcac agcgaagtat
     6661 tcgcgaaagt gatcgtaaac gtagtagctg aataattcgt tattcaacga gacgtaaaaa
     6721 cgccgaccag tggtcggcgt tttgctttta agtgataaaa accgcagaaa cagacgttcc
     6781 ctccataaat ctcttgctat tccttttata aaaagttcgc agtacccata cctggccccc
     6841 agcctcgccg ctggtagttc gtgtcatgtt ttttacgcgt aaaacacgac aaaagcatca
     6901 atacagcgat cgtctcactt gtatgataat aa
//
LOCUS       DAZUCC010000231         6902 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZUCC010000231 DAZUCC010000000
VERSION     DAZUCC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.232"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1071
                     /locus_tag="ACHY09_004362"
     CDS             <1..1071
                     /locus_tag="ACHY09_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFS3887245.1"
                     /translation="EGRLTSSVVQNDIAIYYLFRQMSLCVLIFLALVNKVSENTKQRN
                     LFSKKVTLCITLFFVLGGPIVAHIFSSHYESYDLHIAELTNKNGQVVWKASYVTIMIF
                     MWLTLLSVNLYFNGLRCDIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIEVVS
                     KLTVLVIFMCHIFSALRVTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKKPYCVMIM
                     DIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDTECA
                     KALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALYEAK
                     ETGRNKVVVKEAEILINRKDDE"
     gene            complement(1174..1261)
                     /locus_tag="ACHY09_004363"
     tRNA            complement(1174..1261)
                     /locus_tag="ACHY09_004363"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1225..1227),aa:Ser,seq:gga)
     gene            1494..2432
                     /gene="ghrA"
                     /locus_tag="ACHY09_004364"
     CDS             1494..2432
                     /gene="ghrA"
                     /locus_tag="ACHY09_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFS3887246.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            2487..3224
                     /gene="ycdX"
                     /locus_tag="ACHY09_004365"
     CDS             2487..3224
                     /gene="ycdX"
                     /locus_tag="ACHY09_004365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFS3887247.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3248..3802
                     /gene="ycdY"
                     /locus_tag="ACHY09_004366"
     CDS             3248..3802
                     /gene="ycdY"
                     /locus_tag="ACHY09_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFS3887248.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3904..4395
                     /gene="ycdZ"
                     /locus_tag="ACHY09_004367"
     CDS             3904..4395
                     /gene="ycdZ"
                     /locus_tag="ACHY09_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFS3887249.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYAITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(4459..5292)
                     /gene="csgG"
                     /locus_tag="ACHY09_004368"
     CDS             complement(4459..5292)
                     /gene="csgG"
                     /locus_tag="ACHY09_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFS3887250.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5319..5735)
                     /gene="csgF"
                     /locus_tag="ACHY09_004369"
     CDS             complement(5319..5735)
                     /gene="csgF"
                     /locus_tag="ACHY09_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFS3887251.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5760..6149)
                     /gene="csgE"
                     /locus_tag="ACHY09_004370"
     CDS             complement(5760..6149)
                     /gene="csgE"
                     /locus_tag="ACHY09_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFS3887252.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6154..6804)
                     /gene="csgD"
                     /locus_tag="ACHY09_004371"
     CDS             complement(6154..6804)
                     /gene="csgD"
                     /locus_tag="ACHY09_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFS3887253.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1773 a         1535 c         1690 g         1904 t
ORIGIN      
        1 gaggggcgtt tgacaagcag tgttgttcaa aatgacattg caatttatta tttgtttcgt
       61 cagatgagtt tgtgcgtatt aatatttctg gcattggtaa ataaagttag tgaaaacaca
      121 aaacagcgca atttgttttc caaaaaagtg actttgtgca taactttgtt ttttgttctt
      181 ggggggccga tagttgctca catattttct agccattatg agagctacga tttacacatt
      241 gccgagttaa ccaataaaaa tggtcaggtg gtttggaaag cctcatatgt taccataatg
      301 attttcatgt ggttaacctt gctttcggtt aatctatact ttaatggttt acgctgtgat
      361 atctggaacg gggtgtcggt gattgctttt tgtgccgtgc tgtacaatat ctctctgtta
      421 tttatgagca ggtatagcgt gtccatttgg tatattagtc gcactattga agtagtcagt
      481 aagttaaccg ttttggtaat ttttatgtgc catatattca gtgcgctacg agtaacaaag
      541 gacattgcac atcgcgatcc cttaaccaat atatttaaca gaaattattt ttttaatgaa
      601 ctgacggttc aatcagcatc ggccaaaaaa aaaccttatt gcgtcatgat tatggatatt
      661 gaccacttca aaaaagtcaa cgacacctgg ggacatccgg ttggcgatca agtgataaag
      721 acagtggtga atatcattgg gaaaagcata cgaccagatg accttttagc gcgcgttggc
      781 ggcgaagagt ttggcgtctt gctgacggag atcgatactg aatgtgcgaa agctttagcg
      841 gaaaggattc gggaaaatgt tgaacgttta actggcgata atcctgaata cgccatacca
      901 caaaaagtga caattagtat tggcgctgtt gtaacgcaga aaaatgagtt aaaccctaag
      961 gagatctatc aactggctga taacgcactt tacgaggcga aagagaccgg gcgtaataag
     1021 gtggtagtaa aagaggcaga aattcttatc aaccgcaagg atgatgaatg agtctgtcgc
     1081 tagttgtcga cctccgcctg gggcttctca tcccccagtg tgtgaaatat acgaaaaaaa
     1141 gctcgcactt tcgtacgagc tcttctttaa atatggcggt gaggggggga ttcgaacccc
     1201 cgatacgttg ccgtatacac actttccagg cgtgctcctt cagccactcg gacacctcac
     1261 caaattgtcg ttcctgtctt gctggaacgg gcgctaattt agggaaatca tgacctgagg
     1321 tcaacaaact tttttaaaaa atcgctcgat tattcaaact tcaatcaata tgcagtttta
     1381 ataagcgaaa tttgcttttt ttgccaccga ccacggattt gttatgctgc tggtctttgt
     1441 agatcataac gataagtgcg aataaatttc gccaacactc ttcgggagtc agtatggata
     1501 tcatctttta tcacccaacg ttcgataccc aatggtggat tgaggcactg cacaaagcta
     1561 ttcctcaggc aagagtcaga gcatggaaaa gcggagacaa tgaatcagcc gattatgctt
     1621 tagtctggca tcctcctgtt gaaatgctgg ctgggcgcga tcttaaagcg gtgtttgcac
     1681 ttggggcagg tgttgattct attttgagca agctacaggc tcaccctgaa atgctgaagc
     1741 cttctgttcc actttttcgc ctggaagata ccggtatggg cgagcaaatg caggaatatg
     1801 ctgtcagtca ggtgctgcat tggtttcgac gttttgacga ttaccgcatc cagcaaaata
     1861 gttcgcattg gcaaccgctg cctgaatatc atcgggaaga ttttaccatc ggcattttgg
     1921 gtgcaggtgt actgggcagt aaagttgcac agagtctgca aacctggcgc ttcccgctgc
     1981 gttgctggag tcgaacccgt aaatcgtggc ctggcgtgca aagctttgcc ggacgggaag
     2041 aactctctgc atttctgagc caatgtcggg tattgattaa tttgttaccg aatacacctg
     2101 aaaccgtcgg cattattaat caacaattac tcgaaaaatt accggatggc gcgtatctcc
     2161 tcaacctggc gcgtggtgtt catgtcgtgg aagatgacct gctcgcggcg ctggatagcg
     2221 gcaaagttaa aggcgcaatg ctggatgttt ttaatcgtga acccttaccg ccggaaagtc
     2281 cgctctggca acatccacgc gtgacgataa caccacatgt cgccgcgatt acccgtcccg
     2341 ctgaagctgt ggattacatt tcgcgcacca ttgcccagct cgaaaaaggg gagagggtct
     2401 gcgggcaagt cgaccgcgca cgcggctact aataaagcat caggtttcct gctatccttg
     2461 gcgggaattg aatacaggag agagttatgt atcccgtcga ccttcatatg cataccgttg
     2521 ccagcacgca tgcatatagc acattgagtg attacattgc ccaggccaaa caaaagggca
     2581 ttaaactttt tgcgatcacc gatcatggcc cggatatgga agatgcgccg catcactggc
     2641 acttcattaa catgcgtatc tggccgcgag tggttgatgg ggttgggatc ctgcgcggca
     2701 tcgaagctaa cattaaaaat gttgacggtg aaattgactg cagtggcaaa atgtttgatt
     2761 cgctggatct gattattgcc ggttttcatg agccggtgtt tgcgccgcat gacaaagcga
     2821 ccaatacgca agcgatgatc gccactatcg ccagcggcaa tgtgcatata ataagccatc
     2881 ccggaaatcc gaaatatgaa atagatgtta aagccgttgc tgaagcagcc gcgaaacatc
     2941 aggtggcgct ggaaatcaat aattcctcat ttttacactc acgtaagggc agtgaagaca
     3001 actgtcgggc ggtagccgca gcggtacgtg acgctggagg ttgggtggca ttaggctcgg
     3061 attctcacac tgcgtttacc atgggggaat ttgaagagtg tcttaaaatc ctcgacgcgg
     3121 tagattttcc gccagagcgc attttgaatg tttctccgcg ccgcttactt aacttccttg
     3181 aatctcgcgg tatggcaccg attgcggaat ttgcagacct ttaattacta gtaatggaaa
     3241 tatattaatg aatgagtttt ctatcctctg tcgtgtgctg ggttcgctct attaccgcca
     3301 accgcaagat cctttactgg tgccgttgtt taccctgatt cgtgagggga aactggctgc
     3361 gaactggcca ctggagcagg atgagttact gacgcgttta cagaaaagct gtgatatgac
     3421 gcaagtctct gccgattaca atgcgttgtt tatcggcgat gaatgtgctg tgccgccata
     3481 tcgtagcgca tgggttgagg gcgcaacgga agcggaagtg cgcgcttttc tttccgaacg
     3541 agggatgcca ctagcggata cgccagccga tcacatcggc acattgctgc ttgcggcttc
     3601 ctggctggaa gatcagtcaa cggaagatga gagcgaagca ctggaaacac tgttcagtga
     3661 gtatctgtta ccctggtgtg gtgcgttcct tggcaaagtg gaggcccatg caactacgcc
     3721 tttctggcgc accatggcac cgctaacccg cgatgctatt agtgcaatgt gggacgagct
     3781 ggaagaagat tctgaagagt aaatgtgatc tgcatcactc ataactgcaa cgggtaattt
     3841 gttgttgcat aaaatgtgtg ctcgatctca ttcatggccg cgttttctgc tatcatgcgc
     3901 ggcatgaaca tacttctctc tattgcaatc acaacaggca ttctctccgg tatctgggga
     3961 tgggtggctg tttccctggg cttactaagc tgggcgggct tcctgggctg tacggcctac
     4021 tttgcctgcc cacaaggtgg gctgaaaggg ctggcgatct ccgctgcaac cctgcttagt
     4081 ggcgtggtgt gggcgatggt cattatttac ggtagtgcac tggcaccaca tctggaaata
     4141 ctcggttatg ccataaccgg tattgtcgcg tttctgatgt gtatccaggc caaacagctg
     4201 ctgctttcat ttgttccggg gacgtttata ggcgcatgcg cgacgtttgc cgggcagggt
     4261 gactggaaac tggtgttacc ttcgctggcg cttggactgg tatttggtta cgcaatgaaa
     4321 aacagcggcc tgtggttggc ggcgcgtagt gcaaagacgg ctcaccgtga gcagcaaatc
     4381 aaaaataaag cgtgaggggc actcacgctt tcgcttaaac cgtaaaatgt cggatgataa
     4441 ttccggcatt tttatctatc aggattccgg tggaaccgac atatggcggt atttcactag
     4501 aatgtcattc tgccgttctg ctttattttg caaatcccaa agaccgcggt cgataccatc
     4561 attaatcagg aaaatgacgc ctgtttcgat agccgacatc aggcacagca taacgggttc
     4621 gttcgaggtg taacctactt ccccttccag caagcgctgg tagtcaataa agcggaagac
     4681 ccccgcctga acttcatagg aaagtatcgt cttactggtg ttcaccgagg agaggatctc
     4741 gccggtactc acattgacga cgcgcaggtt cacggcaatc tgatcaagct ggtattgcgt
     4801 gtctgcaccg atcccaaaat atcttgcccc aaccccgccc gatttaacgt tgctttcata
     4861 accgataatt gaaccttcaa ccatgatatt tgccgccgtt aaagattgca gcgggattcg
     4921 gttattaatg gcaaccgtgc cgttttcttg tgccgcacga ataatcttgc gctcgttaag
     4981 caggttttgt aagccttggc gctccagcgg tataaaccag cgagaatctt tcaatgccgt
     5041 gaccagcatt gccgtggcgc tttgcggaac agcagtggag aagttacttg ccgggtaggg
     5101 tttaaattgc ccggtttcgt cctgaatgtt gtataccgaa acaaagatct tacccgtcgg
     5161 cgctggcaga tgggtcaaat ctttgtagct ctgagcacga ggcattaatg tcggtctggc
     5221 ggcttcttta ggcggggcgg ttaagcatcc gctcagtaac atgacggcaa ccaaaagaaa
     5281 taagcgctgc atgattattt tccttatgaa gctggggctt aaaaatcggt tgagttattt
     5341 tgtaaacccg aaacctggat ggtcgaggtt tgtccggttt tacgatctgt cacgttcaac
     5401 tgcaattgac catcgcggtt agcaatatca acaatataat cgttggtcac catgcggccc
     5461 ggtttaccgg tattaatatt cgacagtagc ccacccaaaa tttgtgactg gatggcctga
     5521 gtaaagttat ctaacgccga gggcgtttca ataccaaagt catcgttata gctcggatct
     5581 ttataagagt tttgggcctg agcgctattt aacaaaaaag cgccattatt tgggttacca
     5641 ccaaagtttg gattacggaa ctggaaagtc atggttccag cccaacttaa tggcgaaata
     5701 agcatgagta gaactactgc atgtttgaca cgcattgcag cctccgaaca atttattatt
     5761 taaaattcat catgcgccaa atcgcccgta cttaataacg cttggtttat ctggcgacga
     5821 tttagtgctt cttcagtttg aatcagtgca aagacgacag ttttctcgaa gtctcttttc
     5881 aacggaaata aaaaagtctg gaaaataacg tcctgattca ccgttattgt gatccagctt
     5941 ccccatcgtg cactgggcct ttcattaatc gttaagttac ccgtatagtc actttcccat
     6001 ttatcactaa aggctcggta aaaatcatgg ccaatagatg aaacagtatg gtcagttaac
     6061 aatcccggga cttctacctc aacggcgtga agattccctg cggcgaacag aaattctgcc
     6121 gccacaatcc agcgtaaata acgtttcatg gctttatcgc ctgaggttat cgtttgccca
     6181 ggaaaccgct tgtgtccggt tttttacggc tatcttcttg aaaagattat aaagatgcgt
     6241 tttaaccgta ttttcgctga tgaacaacga acgagcgatc tcgttattag acgcgccgat
     6301 acgcagctta ttcaggatct ctttttcccg atgagtaagg agggctgatt ccgtgctgtt
     6361 ataacgatag ttacctgaat gcgtaatcag gtaactggca agcttttgcg taaagtagca
     6421 ttcgccgcgc aggacgcctt gcaacccatt gacaacacgt tcttgatcct ccatggcata
     6481 aaaaacgccg ttgatatgag gccagttttc aatgtcgcgg tacgggtaat cttcaggcgt
     6541 atttagcaac aatattttga tattgttgtt tttcctgctc aaagtatcct gccaataatg
     6601 gataagcttt ttatccgctt ccatcatatc cagaagaata atagagcctg aagagatatc
     6661 gtccagagaa cgttgaatat tatgtaattt tcctgtaatt gccagcgatt gcttaaggtg
     6721 ctgcaagaga gctgtcgcct gcaaagaagg tttagtgatc aacaataatg tatgaccatg
     6781 aatactatgg acttcattaa acatgatgaa accccgcttt ttttattgat cgcacacctg
     6841 acagctgcct ctaaaataga agcaccagaa gtactgacag atgttgcact gctgtgtgta
     6901 gt
//
LOCUS       DAZUCC010000232         6817 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZUCC010000232 DAZUCC010000000
VERSION     DAZUCC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.233"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(335..2620)
                     /locus_tag="ACHY09_004372"
     CDS             complement(335..2620)
                     /locus_tag="ACHY09_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFS3887254.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAITGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELCDAWVDA
                     HGEKESLFTDEAQTHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIRITRTTDWRIVPKVPVVQPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKYGLRTPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSTVMARI"
     gene            complement(2610..2885)
                     /locus_tag="ACHY09_004373"
     CDS             complement(2610..2885)
                     /locus_tag="ACHY09_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFS3887255.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2882..3106)
                     /locus_tag="ACHY09_004374"
     CDS             complement(2882..3106)
                     /locus_tag="ACHY09_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFS3887256.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGVSALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            complement(3106..3408)
                     /locus_tag="ACHY09_004375"
     CDS             complement(3106..3408)
                     /locus_tag="ACHY09_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFS3887257.1"
                     /translation="MSCSHSVVLLNNALKIAVMENGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            complement(3408..3632)
                     /locus_tag="ACHY09_004376"
     CDS             complement(3408..3632)
                     /locus_tag="ACHY09_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFS3887258.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3696..4196)
                     /locus_tag="ACHY09_004377"
     CDS             complement(3696..4196)
                     /locus_tag="ACHY09_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFS3887259.1"
                     /translation="MTVMTLNLVEKQPATMRRIIGKHLAVPRWQETCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4193..4363)
                     /locus_tag="ACHY09_004378"
     CDS             complement(4193..4363)
                     /locus_tag="ACHY09_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887260.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4374..4649)
                     /locus_tag="ACHY09_004379"
     CDS             complement(4374..4649)
                     /locus_tag="ACHY09_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFS3887261.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            4771..5070
                     /locus_tag="ACHY09_004380"
     CDS             4771..5070
                     /locus_tag="ACHY09_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3887262.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            5186..6199
                     /locus_tag="ACHY09_004381"
     CDS             5186..6199
                     /locus_tag="ACHY09_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS3887263.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(6476..6793)
                     /gene="yegR"
                     /locus_tag="ACHY09_004382"
     CDS             complement(6476..6793)
                     /gene="yegR"
                     /locus_tag="ACHY09_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFS3887264.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAVHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         1814 a         1733 c         1487 g         1783 t
ORIGIN      
        1 ttatgataaa aattaattat ttttatcata aatgatagcg taaacatagt cttatgaacg
       61 acatcagccc tgaagcttca aaactatcat ttacgtttgc gaggcaccat ctatcacatt
      121 gatttataat aagtatcttt atcttcactg cattgctctc aaaaaatcac atagatggaa
      181 attccccgtt gacacactcc caagtgatgc agatacattt caagtgcttc atacacatga
      241 taacggctat ccccctaaat tctgtgcgtt tcttttccct ccagcacaag gttgaaatat
      301 cttgcgtgca gctttacttt gtgaatcttt gtcattatat ccgcgccatt actgttgaga
      361 atcccggcca ctcatcagcg accgggtacg tgaatttttt cccgtcataa tttacggtcg
      421 cgccacgcgc cagcgcctca agctcccatc gctgcggcct gataccgttc tgagcaaggt
      481 caacgcggat acgggtaatt tgcattcttt ccgaccgggt cagtctggcc gatggtgcaa
      541 tttcatgtgg ttttaacggg cttccgtttc tctgctgacg atttggggtt ctcagcccgt
      601 attttaatgc gcccctgagc gccctcacga cctccgggtc attccattcg ataacaccgt
      661 catcaaccag attaagcact gctgcggcgt gttcagaagg tgtgggagcc ggtaacgaag
      721 tatcaccacc ggtgagcttt ccacagttat tgacaggact ccgaggcgcg gcgatgccgc
      781 tttttaaagt caaaggctga acgaccggaa ctttcggcac aatgcgccag tccgtcgttc
      841 tggtgatacg aatatgacgc gcgccgagat gcggcgcgta aatgccgacc actctctcga
      901 cctcttcctc gtactcgtta acgtcatccg aggggctacg ggcgaccctg acagtctggc
      961 aatcgcgcgg gacatttgcc ccaccctgcg cgctgatata cagcgcaaaa tcgccactgt
     1021 ctgcggcagc gcgtgcagcc tcgacgcgtt cgtcaaactc atcagcaatg ctgacgccgc
     1081 gaggcaattt gcgtagttca cggtaagccc ccattgtcgg caggccaacc gttttaaatt
     1141 gaggaatgcg ccacgttgac gcccatgcgg taacagccgc ggcagtgtct ttcagcggcc
     1201 tgccggtgtc gttatcgagc tgaccatcca gtgcatagcc atcgatgttt tttgaaatgt
     1261 atttcgcgat atatcccgca gcaccgcccc ggttaaggtg ttttgcctga aaacggtttc
     1321 gcgcggctcc tctttcgtcg ccatcctctt tgagcgcgta gcgacgcatg atttcgataa
     1381 tctgattacg ctggcgtgga ttacaaaaaa gcatcatatg ccagtgcggc gttccgtcgt
     1441 ggtgtggctc gacgacacgc aaaccgtaga cctgtaaatc attatccttg aatgccgtgc
     1501 gcatcaggct ccagatgcgg cagagataac gctgcgcatc ctttggatta aatgcctcat
     1561 cgttccagcc gtgattaagc tgcacggttt tactttcgcc ttttccgacc tgacgtgtcg
     1621 ggtgatactt tgacggtgcg gtcagcgtga taaacatccc cacatcgccc tctgcggcgg
     1681 cgtaacgctc aataccggca atggtgttca tcagctccat ccggcgaatt tcaggattag
     1741 aaatactgcc catcacctta ctgataaggt cgatgcgctc gccggtttcc ctgttttcga
     1801 ggtcacacga tttaagaaat tccagatttg cctggcggcg cgcacgcaca tcacgaatgg
     1861 catgtttact ggcataagga gaacggtctt tattgacctc cccgacagca atcagtaacg
     1921 cctcatgcca gcgcatacgc tggcctttaa gctgactaat ccaccactca tcgttaaaca
     1981 gacgggcaat ggcagaatat gcctgcctcg tggtcatctg ccctttacgg tattttttcc
     2041 agtagagcgg ggaaatattg aaagcacgtg cagcgccagc gacatgacca tacaggtgag
     2101 tctgcgcctc atccgtaaac agcgattctt tttcgccatg tgcatccacc caggcatcgc
     2161 agagttcctc atacatcatg aaaagctgcg atgagatacg ggcggcaaac tttttcagct
     2221 ccttgtcatt cattccaggc aggcgcgcat aatggtcacg ctctgccaga aacagcaacg
     2281 acgcgtcggt gttcatttca tggcgctgat tcacgcgctc aatgcgcggc cataaacgac
     2341 gctgaaaagt ggatgtgagg aaataaaacc cgtgcaccgg gcttttattg cgccggatgt
     2401 agtcataacg tgaagtaaac agcgagcgca aaaagtaagg caggcggtta atcgtggata
     2461 aaacaccttg cacctgacgc atctcgtcac gtgtaagggg tctttcgcgc ccgacagcct
     2521 cgcgtggcgc gttccatgca taagcaccgg taatcgcctt accggtgcct gcggcaaatg
     2581 ctgacggagg gacaaaacgc ccggaggctt taacggccat atgagccaaa agcctcagaa
     2641 caacgcttgc tgagttgctc aacctgcgcg tttaaatcag caaaagactt tgcgcttccg
     2701 gtcagaatat cgtgatgcat caggccggaa acgagctggc ttaatttcgg gtaataacca
     2761 accaccgcca gccattcctg cccggcgttt ttaccgcttt ccgctctctt tttctcgtgg
     2821 agaataaact gaaagctgtc actggtaacg acataacgtt cgccaatttc gatacgaata
     2881 ctcatgccat tctccggtaa tgcttgtttt ttgcttcaaa gactgactgg caggaaacac
     2941 aacgcgtggc tgacggataa gctgcacgac gggcagcagg tattggcgcg tcacactctt
     3001 cgcaaaccag cgcagaaaca ccgcaatgtt ttacccttgc cgcgttaatc tggcgctcca
     3061 gtaattcagc ctgttgttcc tgaataaaat ctatgttgtc cggcattacc agctcctttt
     3121 gtcgttcagc ttcttaaatt catcagcgca atagctggcg atttctgtcg ttaattttgt
     3181 cagttcatcc acggatgaaa tttgcttgtg gaatacagca cgtttaacaa gtaaattgac
     3241 cacatcagac aggaggttta attcattctg ataaatcgcg ataacagatt cagttatgtc
     3301 gcgcttttct ttatcaagac aaagttgaat aagagacaaa tcaccatttt ccataacggc
     3361 gatttttaag gcgttattca gtaatacaac tgaatgagaa caggacatca aagtacctcc
     3421 ccgcgagaca atccgatatt gtgaaatttt tccgactcct gactgagcag ctcgactatc
     3481 tccacgcggg ataactccgc ctttgtgatg tggcgaatca tggcgtcaag atgagaagaa
     3541 aagcgcgtag ctgcatctgc ctgtgcttcg gttctggcct gttgcagcag taatgcgtat
     3601 ttaccgcact gattttcaga aactgtatgc atgactttct ccaggcaaaa agaagccccg
     3661 cacaattaag tgcgttaaaa actctggtta attacttaat gcagatattg ctctggtttt
     3721 accgatgtca gaattgtcgg tgcatactca aacagactga ataattcacg taatgcacgg
     3781 aataaagcat cacgccagta acatgattct tcattaattc gccagtacgg ctggttgaat
     3841 tctttttctg tcagtcgtgc gtgcataaat aaagtacgac gctggctgac tgttaaaaaa
     3901 ctaatatatg catactcact tgcaccaacc tgacggcgtt ttgagaatgc cccacgcaat
     3961 tcatcaattg cacataccag tcgttcacgt tcgacgtcgt tcatttcttc aaaacgcatc
     4021 gttgcgtgac gctgttttaa ctgcgcatga aagcaaaccg ttagccgttc gcgttccatc
     4081 atctgattat aataatcgca tgtctcctgc cagcgaggga cggccagatg cttaccaatt
     4141 atccggcgca tagttgctgg ctgtttttca acgagattga gcgtcatcac tgtcatttcc
     4201 ataccctccg gcttttcaga aaggtcagag ccttttttaa cggactctgt tttttggtgc
     4261 ggataatgat tcccttacgc cccttaccgt gggtgatggt gaagtcaatc gccctggggc
     4321 tttcgttacg caataactga gcaatacaac gaggctcatt catacggttc tccttaacgt
     4381 ggttcaccga gacctaacca catcaaccag ccgtcacgaa tctctttagg acggctttca
     4441 taagccagtt ttagtccgtt attccatgcc ggaaggtata cccaatattc accagcacgc
     4501 cccgatactg actgagggtc agtaatctca ataactggta atttcccttt ctcaatcata
     4561 ccccttacag ctcttggagt tttaccaatg agttttgcaa actcctgata aggcacggca
     4621 tcagtcacgc ttacaagctg tctattcatc tgctacgatt ctcccttagt gcttctaatg
     4681 gctcctaatg gctaattatt gcctaaaagg ataactccag aagcacaaca tttcacacta
     4741 tcagcaagaa attacgcaat cggagtaatt atgtcaatag acgtttcgga gaagttgaag
     4801 ctaatccgtg aatctgaaag gttaaaccgt aaagaattca gtgaattaac tggtgtagcc
     4861 tacagctcac tttcgagcta tgagagccgg tcaaaaaacg ctggagttga agccataatg
     4921 aaggtcttac aacatcctag atttactaaa tatactttgt ggttcatgac tgatcaggta
     4981 gctccagaag ccgggcaaat tgcgcccgct ctcgcacact ttgggcaaaa cgaaacaacg
     5041 tcgccccact ccggtcaaaa gactggttaa caatttatcg tgaatatatt catcacaagt
     5101 gcctactatt ggtggctaaa tttcagccac cacgaaaaaa gcgattagta gtagcaaaaa
     5161 aaagtaccac tcggagggtt ttctgatggc aatcaaaaaa ctcgatgatg gtcgatatga
     5221 agtggacatc cgccctactg gacgtaacgg aaaacgcatc cgtaggaagt ttgataagaa
     5281 aagcgaagct gtcgctttcg aaaaatacac gttgtacaac caccacaata aagaatggct
     5341 atcaaaacca acagacaaac gacgtctgtc ggaactgaca cagatctggt gggatttaaa
     5401 gggtaaacac gaagagcatg ggaaatctaa tcttggaaaa attgaaatct tcacaaaaat
     5461 aacgaatgac ccatgcgcat ttcaaatcac gaaatccctt atcagccagt actgcgccac
     5521 ccgaagaagt cagggtatta aaccttcgag tatcaatcgt gatttaacat gtattagcgg
     5581 catgtttaca gccctgattg aagcggagtt attctttggt gagcacccta tcagagggac
     5641 aaagaggctt aaggaggaaa aaccagaaac aggctatctc acacaggaag aaattgcctt
     5701 actgcttgct gctcttgacg gcgacaacaa aaagattgcg attctttgcc tgagcactgg
     5761 agcacgttgg ggagaagcag ctcgtttgaa agcagaaaat atcatccata accgcgtcac
     5821 gtttgttaaa acgaaaacaa acaaaccacg caccgtcccg atctcagagg ctgttgccaa
     5881 aatgatcgcg gataacaaac gaggtttttt attccctgat gctgattacc ctcgcttcag
     5941 acgaacaatg aaagcaataa aaccggattt gccaatgggg caagccacac atgcactaag
     6001 gcacagcttt gccactcatt tcatgattaa tggaggaagt attatcacgc tacaacggat
     6061 actaggtcac acgcggattg agcaaactat ggtttacgct cattttgcgc cagagtacct
     6121 tcaggacgcc atttctctta atccgctaag aggtggtact gaggccgaga gtgtccacac
     6181 agtgtccaca gtagagtaac gtttaagggc tttcagtggt aatttatgcc gctcaaaccc
     6241 gcattgtacc gttgaaagcc cctactggtg acaccctaaa tctcccttac acgggcttat
     6301 tttttataca taaaccatag agacggattg atcccaggtc accgtcttcc attgaccaca
     6361 tcgatagaat ctcccttcat agcacgatgc ctttcactta ttggcatcgt gctcgcacag
     6421 gttccggtta cgcacagcca gaacgcgcat gtttgatgct taccaaaaaa tattctcact
     6481 ctccacattt gaatgtcaga cgagcgacgc catgtaatcc tgcaccttct gtcttcaggt
     6541 caactatctg catttttttg ccctgagtaa cacagaaatg aactgcatca ttttttacta
     6601 tattttctgc accagatatt ctacccctgg ctaaagaagc ttcggcttcg gtgtagtatt
     6661 ggttatcgag tttacgctga atattacttt tatatgcaag accaaattta ccgatacttg
     6721 tctcatcatt atgtacagca cacccagaca taagaaaaac actaattaat gatatagcag
     6781 ctatcttttt catctcacct tcccccatta aatacca
//
LOCUS       DAZUCC010000233         6757 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZUCC010000233 DAZUCC010000000
VERSION     DAZUCC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.234"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            326..1666
                     /gene="dcuB"
                     /locus_tag="ACHY09_004383"
     CDS             326..1666
                     /gene="dcuB"
                     /locus_tag="ACHY09_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFS3887265.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1744..3390
                     /gene="fumB"
                     /locus_tag="ACHY09_004384"
     CDS             1744..3390
                     /gene="fumB"
                     /locus_tag="ACHY09_004384"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFS3887266.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3513..4163
                     /locus_tag="ACHY09_004385"
     CDS             3513..4163
                     /locus_tag="ACHY09_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFS3887267.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(4160..5515)
                     /gene="melA"
                     /locus_tag="ACHY09_004386"
     CDS             complement(4160..5515)
                     /gene="melA"
                     /locus_tag="ACHY09_004386"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS3887268.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5798..6706
                     /gene="melR"
                     /locus_tag="ACHY09_004387"
     CDS             5798..6706
                     /gene="melR"
                     /locus_tag="ACHY09_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFS3887269.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         1634 a         1814 c         1701 g         1608 t
ORIGIN      
        1 tctataaacc taatgatatc tgccctgaga acaaaaaata gaccgataaa tatcaataag
       61 ataacagcaa acaaaacatt aacatctgcg cagtacaaac tataaaccca tcgccagaga
      121 gtttttctct ctgaaaaagc cgcttatcac agtgcataaa tttgccgctg ctttaatcag
      181 ccaatattca ctgtgaggta tttgctaaag ccggtaacga ccaaacggat atttagtcag
      241 gctctgaaaa cagttcatac aaaacagaac gtgactgtga tctattcagc aaaaatttaa
      301 ataggattat cgcgagggtt cacacatgtt atttactatc caacttatca taatactgat
      361 atgtctgttt tatggtgcca gaaagggtgg tatcgcgctg ggtttattag gcggtatcgg
      421 tctggtcatt ctggtcttcg tcttccacct tcagccaggt aaaccaccgg ttgatgtcat
      481 gctggttatc attgcggtgg tggcggcatc ggcgaccttg caagcttcgg gcggtcttga
      541 tgtcatgctg caaattgccg agaagctgct gcgccgcaac cctaaatatg tctcaattgt
      601 cgcgccgttt gtgacctgta cgctgaccat tctttgcggt acgggtcatg tggtttacac
      661 cattctgccg atcatctacg acgttgctat taagaacaac atccgtccgg aacgtccaat
      721 ggcggcaagt tctatcggtg cacagatggg gattatcgcc agtccggtgt cggttgcagt
      781 cgtgtctctg gttgcgatgc tgggtaatgt cacctttgat ggtcgccatc ttgagttcct
      841 cgacctgctg gcaatcacca ttccatcgac gttaatcggt atcctggcga tcggtatctt
      901 cagctggttc cgcggtaaag atctggataa agacgaagag ttccagaaat tcatctccgt
      961 accggaaaac cgtgagtatg tttacggtga taccgcgacg ctgctggata aaaaactgcc
     1021 gaaaagcaac tggctggcaa tgtggatctt cctcggggca atcgctgtag tcgcccttct
     1081 tggtgctgat tcggacctgc gtccatcctt cggcggcaaa ccgctgtcga tggtactggt
     1141 tattcagatg tttatgctgc tgaccggggc gctgattatt atcctgacca aaaccaatcc
     1201 cgcgtctatc tcaaaaaacg aagtcttccg ttccggtatg atcgccatcg tggcggtgta
     1261 cggtatcgca tggatggcag aaaccatgtt cggtgcgcat atgtctgaaa ttcagggcgt
     1321 actgggtgaa atggtgaaag agtatccgtg ggcctatgcc attgttctgc tgctggtttc
     1381 caagtttgta aactctcagg ctgcggcgct ggcggcgatt gttccggtcg cgctggcgat
     1441 cggcgttgat ccggcataca tcgtggcttc agcaccggct tgctacggtt attacatcct
     1501 gccgacttat ccgagcgatc tggcagcgat tcagtttgac cgttccggca ccacccacat
     1561 cggtcgcttc gtcatcaacc acagctttat tctgccgggg ttgattggtg tgagcgtatc
     1621 gtgcgtcttc ggctggatct tcgccgcgat gtacgggttc ttataaatgc actttgcgtg
     1681 ccgcccgtga ctacgcggca cgccattttc gaataacaaa tacagagtta caggctggaa
     1741 gctatgtcaa acaaaccctt tatctaccag gcacctttcc cgatggggaa agacaatacc
     1801 gaatactatc tactcacttc cgattacgtt agcgttgccg acttcgacgg cgaaaccatc
     1861 ctgaaagtgg aaccagaagc cctgaccctg ctggcgcagc aagcctttca cgacgcttct
     1921 ttcatgctcc gcccagcaca ccagaaacag gttgcggcta ttcttcacga tccagaagcc
     1981 agcgaaaacg acaagtacgt ggcgctgcaa ttcttaagaa actccgaaat cgccgccaaa
     2041 ggcgtgctgc cgacctgcca ggataccggc accgcgatca tcgtcggtaa aaaaggtcag
     2101 cgcgtgtgga ccggtggcgg tgatgaagaa gcgctgtcga aaggcgtcta taacacctat
     2161 atcgaagata acctgcgcta ttcacagaac gcgccgctgg acatgtataa agaggtcaac
     2221 accggcacga acctgcctgc gcaaatcgac ctgtacgcgg tagatggcga tgagtacaaa
     2281 ttcctctgtg tcgctaaagg cggcggctct gccaacaaaa cgtatctcta ccaggaaacc
     2341 aaagccctgc tgacgcccgg caaactgaaa aacttcctcg tcgagaaaat gcgtaccctc
     2401 ggtacggcag cttgcccgcc gtaccatatc gcgtttgtga ttggcggtac gtctgcggaa
     2461 accaacctga aaaccgtcaa gttagcaagc gctcactatt acgatgaact gccgacggaa
     2521 gggaacgaac atggtcaggc gttccgcgat gtccagctgg aacaggaact gctggaagag
     2581 gcccagaaac tcggtcttgg cgcgcagttt ggcggtaaat acttcgcgca cgacattcgc
     2641 gttatccgtc tgccacgtca cggcgcatcc tgcccggtag gcatgggcgt ctcctgctcc
     2701 gcagaccgta acattaaagc gaaaatcaac cgcgaaggta tctggatcga aaaactggaa
     2761 cacaacccag gccagtacat tccacaagaa ctgcgccagg ccggtgaagg cgaagcggtg
     2821 aaagttgacc ttaaccgccc gatgaaagag atcctcgccc agctttcgca atacccggta
     2881 tccacccgtt tgtcgctcac cggcaccatt attgtgggtc gagatattgc acacgccaag
     2941 ctgaaagagc tgattgacgc cggtaaagaa ctgccgcagt acatcaaaga tcacccgatc
     3001 tactacgcgg gtccggcgaa aacccctgcc ggttatccat caggttcact tggcccaacc
     3061 accgcaggtc gtatggactc ctacgtggat ctgctgcaat cccacggcgg cagcatgatc
     3121 atgctggcga aaggtaaccg cagtcagcag gttaccgacg cgtgtcataa acacggcggc
     3181 ttctacctcg gtagtatcgg cggtccggcg gcggtactgg cgcagcagag catcaagcat
     3241 ctggaatgcg tcgcttatcc ggagctgggt atggaagcta tctggaaaat cgaagtagaa
     3301 gatttcccgg cgtttatcct ggtcgatgac aaaggcaacg acttcttcca gcaaatcgtc
     3361 aacaaacagt gcgcgaactg cactaagtaa cctcttcggc ccagcgcctg gcagcatgct
     3421 gccaggtgat ccccctgggc cacctctttt gcgattgtaa tttcacgctt actggtcaat
     3481 agtcagtatt ttccctgact tgcgaactca ccatgacccg cacactcaag ccgttaattc
     3541 ttaacaccgg cgcactggcg ctaacgttaa tcctgattta taccggcatt tcggcccatg
     3601 acaaactcac ctggctgatg gaagtgacac cggtgattat tgtcgtgccg ctactgcttg
     3661 ccaccgctaa acgttatccg ttaactccgc tgctttatac gctcattttc tttcacgcca
     3721 tcatcctgat ggtcggcggg caatacacct acgcgaaagt ccccgttggt tttgaggtgc
     3781 aggaatggct ggggttgagc cgtaatccgt atgacaaact ggggcatttt ttccaggggc
     3841 tggtgcctgc actggtggca cgagagatcc ttgtacgcgg gatgtacgtt cgcggacgta
     3901 agatggtggc gtttctggtc tgctgcgtgg cgctggcgat aagcgcgatg tatgaattaa
     3961 tcgagtggtg ggcggcgctg gcaatgggtc agggagcgga tgattttctc ggcacccagg
     4021 gcgaccagtg ggacacgcaa tccgatatgt tctgcgcgct gcttggcgca ttaacgacgg
     4081 tgatattcct cgctcgcttt cattgccgcc agctacggcg ctttggctta atcaccagag
     4141 cgccggaagc gattacccct taacggtgca accagcctgg cagccagtcg ccgtgggcgg
     4201 caatcaggtc gtcaacaaga gcatatattt catcaatgcc cagcacggcg gcagtatgcg
     4261 ggtccatcat cgcagcgtgg taaacacggt cgcgattttc cgtaagaatg gcttcggtca
     4321 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtaacgtac
     4381 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     4441 cttgtggcag gttatcaatc aatccatcgt tacggacgtt gccgtaaatc acactcggct
     4501 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     4561 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     4621 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgacctg
     4681 gcttaataaa ccacggtgtg tactcagcaa aatgttctga cgattccgtg acgaagtagc
     4741 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtattg ccatgaatat
     4801 tcggcttcgg tgcttgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     4861 aactgccgtc ggcggttttg cgctccagtt ccaggtaaaa cgccatatgg ttgatacctg
     4921 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     4981 ttccctgcac cgaatggcac agccctacct gtttgatatg cggatagcgg gcatacatcg
     5041 cccaggtatt catcgccatt gggttaacgt agttgagcat ggtggcatcg gggcagactt
     5101 ccgtcatgtc ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     5161 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     5221 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     5281 catcctgtaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     5341 aatccatcag cttgcgcacc acaatatgcg actcttccag gcgggtggga tcaatgtcca
     5401 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
     5461 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
     5521 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccgtct
     5581 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
     5641 atgggaataa gttacctgaa aagcagagtt ttcggcctca tgatgctcgg acgctcccct
     5701 cgccgctgtg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
     5761 tgccataatt ctgatattcc aggaaagaga gccatccatg aatacagata cgtttatgtg
     5821 cagcagcgac gaaaaacaga cccgcagccc gctgtcgcta tattcggaat accagcggat
     5881 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
     5941 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
     6001 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
     6061 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
     6121 ggataaagac ctgattaacc acgttactca cggcatggtg atcaaatcac tggcgacaca
     6181 gcaacttagc ccgtttgaag tgcgccgctg gcagcaggaa ttgaacagtc cgaacgagca
     6241 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctgg
     6301 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
     6361 tgcgcaattt tatgtcagcc agatgctggg ctttattgcc gaaaactatg atcaggcact
     6421 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
     6481 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
     6541 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
     6601 tcgttcgagt agccgttttt acagcacgtt cggcaaatat gtcggcatgt caccgcaaca
     6661 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
     6721 tcagccaaaa aaatagttta gccgtcgata ataagca
//
LOCUS       DAZUCC010000234         6750 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZUCC010000234 DAZUCC010000000
VERSION     DAZUCC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.235"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(52..285)
                     /locus_tag="ACHY09_004388"
     CDS             complement(52..285)
                     /locus_tag="ACHY09_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887270.1"
                     /translation="MARSDYDFINLSLEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(380..1003)
                     /locus_tag="ACHY09_004389"
     CDS             complement(380..1003)
                     /locus_tag="ACHY09_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3887271.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKHFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRMVGSENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTSVEVHDNLTGTTRKIPAYHL"
     gene            complement(1092..1601)
                     /locus_tag="ACHY09_004390"
     CDS             complement(1092..1601)
                     /locus_tag="ACHY09_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887272.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCSSFHRRVHERITERDHTVSPSAPVVLRWLREQHEGAPIRCLLLLSQTSVCHP
                     RASVTVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYVALKTAVQSLM
                     PRGIATIIR"
     gene            complement(1882..2060)
                     /locus_tag="ACHY09_004391"
                     /pseudo
     CDS             complement(1882..2060)
                     /locus_tag="ACHY09_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
     gene            complement(2128..2401)
                     /locus_tag="ACHY09_004392"
                     /pseudo
     CDS             complement(2128..2401)
                     /locus_tag="ACHY09_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2448..3014)
                     /locus_tag="ACHY09_004393"
     CDS             complement(2448..3014)
                     /locus_tag="ACHY09_004393"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS3887273.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDKSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            complement(3042..3239)
                     /locus_tag="ACHY09_004394"
     CDS             complement(3042..3239)
                     /locus_tag="ACHY09_004394"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS3887274.1"
                     /translation="MPQDDFCIVNPGGCIIHFPANGRYILDIHDPDFPEQYQRLLERL
                     DYLTKPGTKASGQRNSGYSGF"
     gene            complement(3487..3912)
                     /locus_tag="ACHY09_004395"
     CDS             complement(3487..3912)
                     /locus_tag="ACHY09_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFS3887275.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDSHALSAQQKATCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRATCTKSVYRYQPDLCQDCGGAST"
     gene            complement(3909..4292)
                     /locus_tag="ACHY09_004396"
     CDS             complement(3909..4292)
                     /locus_tag="ACHY09_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFS3887276.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWRCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            complement(4664..5257)
                     /locus_tag="ACHY09_004397"
     CDS             complement(4664..5257)
                     /locus_tag="ACHY09_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS3887277.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRLDSTLSEICSLV"
     gene            complement(6489..>6750)
                     /locus_tag="ACHY09_004398"
     CDS             complement(6489..>6750)
                     /locus_tag="ACHY09_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887278.1"
                     /translation="NKILSSRESKENLIGLFTGRHKYTLLQCIEKLGVLVDYVISPAD
                     DIKNVMMLYGNNAENKNRRRLIYNALTIICGDDTKNNQSDPR"
BASE COUNT         1899 a         1696 c         1463 g         1692 t
ORIGIN      
        1 aagaaccccc tgccggcaat cacactgcct gatggcagca taatccactt attaattctc
       61 atccccggag acaagccctg aaaaccactc gggatgttca ctgtatgctg cgttcagagc
      121 atcccaggaa acgttcatac agaagctgtc ctgcaatttg caggcaacca cctctgccag
      181 gcggttccgg ttatccgcca gtccggcata acctctctct gccagccact cattcagttc
      241 atgttccaga gacagattga taaaatcgta atcggacctt gccattcccg tttccttaac
      301 acatgtcttc agactcaggg ataaagtata aaaatgtcgc cctgctgctg ccagagcaat
      361 attccccctg atggccgtat cagagatggt atgccggtat tttacgggtg gttcccgtca
      421 ggttatcgtg tacctccacg ctcgtagtca ccaccggcat tccggccttt ctcagcctca
      481 aaacatcagc tgcaatacgc tgactaccag accagaacag gccgtccagt gcagtcacca
      541 gtaaccccgc ctccagcgca tacttcagcc gttcacgggg cgctttcact tcccgggcaa
      601 cctgctgata tggcaatgat gtgttttcac tcccgaccat ccggcgaata cgatgagaca
      661 gatgataccg gtatgtatcc ggcacacctg aaaggctggc cttcaggctg tacacgcagc
      721 caaagtgttt atcgttgaac accacatttt tctggctgat atcccattct tcacgcagcg
      781 cggcaatcag ttgtggtgta cgggtaagca acaagcgaaa aggcagttca aaactggtga
      841 cgtaatccac attcagcagg gcaatgcgaa gtcgttcttc tggtccggct tctgtctgcc
      901 ggcactcctc caggacatcc tgccactgca ggcgaagacg ggaagactca ttcagttctg
      961 taaagcagta tttatccgcc agatagtcaa ttcgtgtatg catactgaag agtattccgt
     1021 ataaagattc agctggcaaa actttatcag tctgtaaaaa ctaacggaag agtcgatatt
     1081 tctcccgtcc atcaccggat gattgttgca ataccccgtg gcatcagaga ctgaacagcc
     1141 gtttttaacg caacgtattg ctctgatgta tcaggccgga caacccgaaa acacctttcc
     1201 acccggcatt gtccgccagc gcttatcacc ggccaggtct gttgcagtaa atccgccact
     1261 tgcgaacatg cttcatcaac tgtgacactg gcccgcggat ggcaaacact cgtctggctg
     1321 agcagcaaca gacatcgtat cggtgctcct tcatgttgtt cccgcaacca gcgtaatacc
     1381 accggcgctg atgggctgac cgtgtgatca cgctccgtaa tacgttcgtg cacccgtcgg
     1441 tgaaaggaac tacagaatgt ctgaatctgt tgaccacaga cgccttctgt cgaatgaagt
     1501 gtgaagtgga ttgccagcag atgcggccag tgatccaccg cctgctgaac aaaacgccgg
     1561 atttcccccg gctctgaaag taaggcttcg gttatttgca ctattttatc tctgttgaac
     1621 ttggttaagt cggtgcagac gcatcaacac aagtacggtt cgatgcaaac agctgtgact
     1681 ggcaatatga aaggaatgat gaatcagtca ggatgacaaa gtgccagctg accggagggg
     1741 acgcaggaag attcacgggg ggaccagcac cagggaacag cgccacaata ccaacgctga
     1801 cacgttgaac attgccagcg taccggtatc acaacacgtt tcatacttct gccaccgtga
     1861 ttcttcgatt cgttactgta tctactgtga cacttcgctt ttatacctgc ggctggatcg
     1921 gcccggcttg atgaatcttc actgatcagc ttataaaccc ctctgtcggt cataccggtg
     1981 aaactggtga tatagttcat gtcaatcagg gaattatcgg cacgcagaaa tacgctgtcg
     2041 tggcttgttg tagtcaacat ggtcagaatg tcctctgtga gatttatgaa gattgtgcga
     2101 atgcggggaa tctgctgagc tgtgctttca gaactggcct gttacgggat accagggatt
     2161 accggcgggg taacgggctt ccggatcata cacaccacga ttatcgcgga caaaatcact
     2221 gaacgcccat atcacctctt taagtatgtc ttcgcagtcc ggtacatgac gatccagcgc
     2281 cacatcccgg gtggtactac tttgatgcgc ccggtgacac aaagcccgga ttgttccaga
     2341 catcctgaat caaacgcccc agattagggg cgtcgaaata tgcctctctg accattatat
     2401 tccggcgtac aggtagcagg tcagaagtga caatgcgtca cctgacgtta aaagtcacta
     2461 cacccaagat gacgttcaac agcaccatgc gattcaatgt aagcccgggc tgtctgttcc
     2521 agtacaccag gctcagcgtt gtatgtgtta gctgcatcaa ataccaacga cagcacttca
     2581 ggatacacaa ccagatgtgt aatggagtta tcttcaccca atacttttcc ccacgcctgc
     2641 tcaatcagat ttctgagaac caccacctca cgactcttac accagacatc gttattaagt
     2701 agcagcacca taagataagg agtggtatcg ttagccacag cctccctact ccagagataa
     2761 tataaagggg tgggctcaac agatttatct ttacgtcgct tacactgcaa atattcagaa
     2821 atgagtctat gcagttcacc agtaaaatca gccatcaaag agggaatggc cttattaata
     2881 ccctgccctg gtattaattt aaattgtaat aatttaattt caggatgtgt ggctgcagcc
     2941 cgatacagag ttgcaaggac acacttttgc cagagggcgt tacaggaaag cttaacgttt
     3001 gattctgtat acataataaa tcaccttaca gttacaacag gtcaaaaacc gctgtagcca
     3061 gagttacgct ggcctgatgc tttagtaccg ggcttcgtca gataatccag acgctccaat
     3121 aagcgctgat actgctcagg gaaatcagga tcatgaatat ccaggatgta acgtccatta
     3181 gcagggaaat gaataatgca gccccctgga ttaacaatgc agaaatcgtc ctgaggcact
     3241 gatcaatacg gagaggactc tcgcgtgtgg tttattgaca ccacagtgca gattcggcga
     3301 atccgcgatc acggtgcaat ttcgttccac agcacacaat cataaccccg ggttttattc
     3361 aggtaagcag gactgcggat atccggtgtc gcgcctttgt gtcacgaacg gggtaggtgc
     3421 gaaacaccgg ataaaatgca ggctggcaat acctctgaac gccctgcgca gagcggatat
     3481 tttggattaa gtactcgcac ctccgcagtc ctggcacaaa tctggctggt acctgtaaac
     3541 agacttcgta catgttgctc tgcaataaat ccccgtacca caggcctcac agaatttctt
     3601 cccatgaaaa aactgctcac acatcacaca atgccactcc agcacgaccg gtaatggcga
     3661 tagaaacatc gccatatcct caatgtaagg gtgggacttt tccggattca gcaccacgca
     3721 ggtcgccttc tgttgcgcgc tcagggcatg tgaatcatgc tcaaaccacg ccccctgagc
     3781 atctgtctgc aaaatcaacc gaccacgaca ggaaaggcag aaacactgcc ggatatttct
     3841 gctgagcgca gagtcggcac tgataatgtg ttcacccggc gtgatcccca gccccgtttt
     3901 tataccgttc attcagccac tccctcctca ctgaagtgcc ctgtatggca gtgtgtgcag
     3961 taccgctccc cataataatc gtggtgacat tgtctgcagc gccagctggc tttacgcacc
     4021 acgggtaagg catccgggac gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt
     4081 tccggcctga cataaggaca ttgctgcgcg tgctccgtca gcccttcgtc tgcgtgttca
     4141 aaccagggac gctcagtgtc gtattgcggg tggtatctga gcgcactgcc gcaaaggtgg
     4201 caagtgtagc gatcgtaagg tgcagtctgt gcggtacggg cacccgtcag acgcccgttg
     4261 ccatcaaatg caataaagga ttttgcgtac atcgtcatat tcctcagccg ccagacgaca
     4321 cgcaggcaac agctgtcccg gtacgggcag tgtggtcagg gtgtgaatgg cggttcagtt
     4381 aaaatctgga ctggagtttg ggtaaaacag gctggttgct gttaccgcac tgtggttgtc
     4441 acacaatggc ccacctgcgt aaaaaactgt cagcaggcag ggatggacat cgggggatgt
     4501 gctgtatcgg ttgccggaag gaatggtaaa gaatgtgcca tggttaaatc tccgtatggt
     4561 tcatacggaa tagccatgtc gtaaaaaatg cgcagagtcc ctgaagtggc ctccggaacc
     4621 gactgtaaca attagcgcca gcccgtagtt gtcgttcagg ggcttacacc agagaacata
     4681 tctccgacag ggtactgtcc agtcgcggtc ggccccggtt acttttttcg ggaagcctgc
     4741 tacgccccag aataataccg tgttctttct gatcctgttt agccaggtag ctgagggcgt
     4801 aacgtaagtc gtctaccgca gatttatcac tatagtgaat cacgtgatcg atacgcaccg
     4861 ggtatttgtc tttagcccgg cacagatgaa aataaccctc cccttccgtg atccgcctcc
     4921 agatatctcc cagttgcctg gatatctgat aagagttctt gtgacgttgt ccatccagat
     4981 agccgataaa atgaatatga agccctttgt ctggtgtata ttccattacc cagtaatatc
     5041 ctgctaacat cgtctgggtt tcgctaagta aacggtatat ttccatacac atactgtgct
     5101 tacaggaatg cccgaaactg ggcgtgtctt tcctgtaggc aaaatcaatt ctgaaaggta
     5161 acagtttaga aaaacgttga aacataccgt ccatgtgttc attcacatct ttcagaatcg
     5221 cgaaattcat ttcgtaattg ggattggcat tatacatttt atagtcctta ctttataaaa
     5281 ctcacaggaa gaggaaatta tgtaaatgct gagggtacag aaacgtcgta ctacggcatt
     5341 aataaaagag ataaccagca gcacagacaa cctgataaaa ctgttttgta ttacctcatt
     5401 acagcaatga ggtaatattt cagaatacag tgagactctc ttaaactgag gaccagtaca
     5461 aattacaatg gtaaaaacac cacctcatag aattgagttt aagaaaacac cattcactca
     5521 acctgacatt cacaaaagtc agtaccaggt agcctcaggt aacatcgcta ttaatattat
     5581 cctgccatta agtaaaaaca gaaactctgt gaggaagcct caccaatatt tgaccagtag
     5641 cataaagaat aacgatatga gagatacgcc cacaccagag atgattagcc catacaaaaa
     5701 gataatattt attaatacca ccgaacctca atagagtaat aacacactaa tagcgtttta
     5761 gtacacactt aacactaact atgcaccaga cacatactca ggattaacat tctcaacatt
     5821 tatggtactc tcaaagagta taattcacca cacaggtaaa ctctcacacc agtagtatgg
     5881 agataatgct gtacacatac ttacagagta aagtgtacag ctagcataca gtggcgagca
     5941 gacattacaa cataaagact caagatagca taataaagca taataagaac acaacaaaac
     6001 tcccactaat aatactaatt aaccttaatt cttttttgaa caggaaatca acatagctcc
     6061 agacctcttc aggtcataca cttttgtatt ggatgttcaa tgtgtacttc tgcacagaca
     6121 aaaaaataaa acccacaaaa taaaatatca atcccaataa aaaattttaa aatacagcgt
     6181 gaaacaccac gttatggata tgttttcata tcaccagaag catatttcac acatacaata
     6241 caggaacaat tcatcatata atcagggtta ctgttcactc ctcacggtta tccgcgagac
     6301 gataaaacat gacactaatc acaaaaacga gagatagtat ttgcaaaatg caaacaagat
     6361 aaaaatcaat gtgttgcgat tttttatttt ttcagacatg catgccccct ctgctattgt
     6421 tgctagccga ctagcaacaa tagcagggat atacaaacat cggatgccaa aaacacgttc
     6481 cgataatatc atctagggtc tgactggttg tttttagtat catcaccaca aatgatggtt
     6541 aatgcgttgt agatcagtct gcgtctgttc ttgttttcag cattatttcc atacaacatc
     6601 atgacgtttt taatatcatc agccggtgat attacataat caaccaatac gccaagcttc
     6661 tcaatacatt gcagtaaagt atatttgtgt ctgcccgtaa ataatcctat caggttttct
     6721 ttactttcac gactggataa tattttgttc
//
LOCUS       DAZUCC010000235         6710 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZUCC010000235 DAZUCC010000000
VERSION     DAZUCC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.236"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..239)
                     /locus_tag="ACHY09_004399"
     CDS             complement(<1..239)
                     /locus_tag="ACHY09_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HFS3887279.1"
                     /translation="MRSLFALLITVISCTTASAAMTETCQKYFDETDKFLSQMKTTGV
                     AEGQINLMKQQYEQSKKQFSSLPEASQDAACKQAS"
     gene            complement(993..1241)
                     /locus_tag="ACHY09_004400"
     CDS             complement(993..1241)
                     /locus_tag="ACHY09_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS3887280.1"
                     /translation="MRVEICIAKEKISKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIH"
     gene            complement(1467..1562)
                     /locus_tag="ACHY09_004401"
                     /pseudo
     CDS             complement(1467..1562)
                     /locus_tag="ACHY09_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1559..2125)
                     /gene="yecD"
                     /locus_tag="ACHY09_004402"
     CDS             complement(1559..2125)
                     /gene="yecD"
                     /locus_tag="ACHY09_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS3887281.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            2435..4207
                     /gene="aspS"
                     /locus_tag="ACHY09_004403"
     CDS             2435..4207
                     /gene="aspS"
                     /locus_tag="ACHY09_004403"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS3887282.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKDENN"
     gene            4325..4777
                     /gene="nudB"
                     /locus_tag="ACHY09_004404"
     CDS             4325..4777
                     /gene="nudB"
                     /locus_tag="ACHY09_004404"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFS3887283.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            4806..5546
                     /gene="yebC"
                     /locus_tag="ACHY09_004405"
     CDS             4806..5546
                     /gene="yebC"
                     /locus_tag="ACHY09_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS3887284.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            5581..6102
                     /gene="ruvC"
                     /locus_tag="ACHY09_004406"
     CDS             5581..6102
                     /gene="ruvC"
                     /locus_tag="ACHY09_004406"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFS3887285.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6104..6706)
                     /gene="yebB"
                     /locus_tag="ACHY09_004407"
     CDS             complement(6104..6706)
                     /gene="yebB"
                     /locus_tag="ACHY09_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFS3887286.1"
                     /translation="MNVNYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1709 a         1571 c         1727 g         1703 t
ORIGIN      
        1 tcacttgctt gtttacaggc agcatcctgg cttgcctcgg gcagagatga gaattgtttc
       61 tttgattgtt catattgttg tttcattaaa ttaatctgtc cttctgctac tccggttgtt
      121 ttcatttgcg ataagaactt atctgtttca tcgaaatact tctgacatgt ttctgtcata
      181 gccgcactgg cagttgtgca ggatattaca gtgattaata aagcaaagag acttctcata
      241 tatcctccat tgttttgttt gaaaaattaa cgctattaaa taactcatag acaggtaaat
      301 ttatgtgatt atagtcataa actataaaaa tattgatgtt gtctttgtag aaaggttgtg
      361 agcaattatg aaaatttatt tgcttgttca ctggttttag ggagtttgat gtgtccatga
      421 agcgtttctg acgagaaatc aacataaatc accagttggt gaactaggat tgccagaaac
      481 gctcctgtca gttctgtagt gtgtagtgct cacttagagc ctggtgaact ggataatgag
      541 tcgcacctca gtatcctgca atgggctgct tttcccctgc tcctgattct tttttgtttc
      601 aggtcttacc aacataatgg ttgttgtaat aaattgcgtg tgattctgta tcgggtcaac
      661 catgtgacga tacatgttca gtaaggaaga ctgaagggtt cagagtgcga gtatgattaa
      721 tagatggaat cagcgtttca aagattttga gggaatataa ggtctgacct gatgattacc
      781 ttttgcgcct gtatcctttg cataaaggag gttgatgtag ataaagtcat ctccacttgt
      841 gatataaaac agtcagttat gaaaaatcgg cataaacatc cttttgttaa ccttaaagcc
      901 agacttacgg tttttccata gttacagata agcggctagt aaggttttac aggtgataag
      961 cctgtaacgg aaatcacctg caaatctatt aattagtgaa taaaccagtc atccgctgtt
     1021 tcccagactt cctgaagcgt ttcttgtaca aactcttgcg cggattcttt atccacggct
     1081 cttaaaatag tgagtccatc attgctggca gatttaacga taacatctac atcgtcataa
     1141 cgcttaccga cacgtcgtag catttcctgt tgcagggcag gaacagaacc ttttggcatt
     1201 ttgctgattt tttctttagc gatacagatc tcaacacgca taacaccctc ctgtaactgt
     1261 ggttatgtac aggtgttatt tttatctgta tggataacca gtgtcaattc ctgatgttgt
     1321 ttatggggca tcaatggggc atgtatggga cactttttat cggcgaaatt cgtcgaagtt
     1381 cgtcgacatg gtaaacgaat catctatcca acccttgaaa aacggcgctc ctggacgatc
     1441 ttcgtcgatt tttaaaaatg ttgcgtcacg cagttaaagt ggcgggcata ctcttcaagg
     1501 ttggtgatcc ccaaccgcac ccatttagga tgcgaccatt gcggcagacc gatgtaaatc
     1561 ataacgcgtg gaggatctct tccacactgc gcacacgggc gatgcgcggg tagatatgat
     1621 taatgctgtt attgtgctgc tcggcgctgg cggcgctaca ggcatcttcg gcaatcacca
     1681 gattaaaacc gagttcccag gcattgcggg cggtggattc aacaccgata ttggtcgaga
     1741 tcccacataa cactattgta tcgatacccc ggcggcgtaa ttgcaactcc agatccgtac
     1801 cgtaaaacgc accccattga cgtttgatga tttcgatatc gctgtcggtt gcacctaatg
     1861 cagcaggatg ttgccaccag ttttcgggca acacttttgc gggggagggg gcatcaaccg
     1921 gctgttttaa tgcttcggcg taatcggcag accagccaac gcgcaccaga aacacgggct
     1981 gaccgctggc gcgaaatttc gccgccagct tcccggcgcg attaaccacc tcatcggcag
     2041 tatgtggacc tccggcaaaa ggtaagatgc cttcttgtaa atcaatcacc accagcgcgg
     2101 tggttttagc attaagttca agcatgatga ctcctgacaa aacaaaatcg tgcctctcac
     2161 cttaatgcga ttagccgatg gcggaacgac aaattttgtt aatttttatg agttcgggca
     2221 acacctttat agcaaactgg cgtagagtct gatttcaggc ccgatataaa gtggtaacga
     2281 ggcgttccag cgcgggccag gctcttacgg tgcggcagaa tttccagtat aatagccgcc
     2341 ttttttcatt cagttgtgac atacagctaa cgctgcgact tggtcacctg cgcagcaggg
     2401 ggcatccgcc ggcagctaag ttaagggata tctcatgcgt acagaatatt gtggacagct
     2461 ccgtttgtcc cacgtggggc agcaggtgac tctgtgtggt tgggtcaacc gtcgtcgtga
     2521 tcttggtagc ctgatcttca tcgatatgcg cgaccgcgaa ggtatcgtgc aggtattttt
     2581 cgatccggat cgtgcggacg cgttaaagct ggcctctgaa ctgcgtaatg agttctgcat
     2641 tcaggtcacg ggcaccgtac gtgcgcgtga cgaaaaaaat attaaccgcg atatggcgac
     2701 tggcgaaatc gaagtactgg cgtcctcgct gactatcatc aatcgtgcag atgttctgcc
     2761 gcttgactct aaccacgtca acaccgaaga agcgcgtctg aaataccgct acctcgacct
     2821 gcgtcgcccg gaaatggctc agcgcctgaa aacccgcgct aaaatcacca gcctggtgcg
     2881 ccgttttatg gatgaccacg gcttcctcga catcgaaact ccgatgctga ccaaagccac
     2941 gccggaaggc gcgcgtgact acctggtgcc ttctcgtgtg cacaaaggta aattctacgc
     3001 gctgccgcaa tccccgcagt tgttcaaaca gctactgatg atgtccggct ttgaccgcta
     3061 ctatcagatc gttaaatgct tccgtgacga agacctgcgt gctgaccgtc agcctgaatt
     3121 tactcagatc gatgtggaaa cttctttcat gaccgcgccg caagtgcgtg aagtgatgga
     3181 agcgctggtg cgtcatctgt ggctggaagt gaagggcgtg gatctgggcg atttcccggt
     3241 aatgaccttt gcagaagcag aacgccgtta tggttctgat aaaccggatc tgcgtaaccc
     3301 gatggaactg accgacgttg ccgatctgct gaaatctgtt gagtttgcag tatttgcagg
     3361 tccggcgaac gatccgaaag gccgtgttgc cgctctgcgt gttccgggcg gcgcatcgtt
     3421 gacccgtaag cagatcgacg aatacggtaa cttcgttaaa atctacggcg cgaaaggtct
     3481 ggcttacatc aaagttaacg aacgcgcgaa aggtctggaa ggtatcaaca gcccggtagc
     3541 gaagttcctt aatgcagaaa tcatcgaagc catcctggat cgtactgccg cgcaagatgg
     3601 cgatatgatt ttcttcggtg ccgacaacaa gaaaattgtt gccgacgcga tgggcgcact
     3661 gcgcctgaaa gtgggtaaag accttggtct gaccgacgaa agcaaatggg caccgctgtg
     3721 ggttatcgac ttcccgatgt ttgaagacga cggtgaaggc ggcctgacgg caatgcacca
     3781 tccgttcacc tcaccgaaag acatgactgc tgcagaactg aaagctgcac cagaaaatgc
     3841 ggtggcgaac gcttacgata tggtcatcaa tggttacgaa gtgggcggtg gttctgtgcg
     3901 tatccataat ggtgatatgc agcagacggt gtttggtatt ctgggtatca acgaagagga
     3961 acagcgcgag aaattcggct tcctgctcga cgccctgaaa tacggtactc cgccgcacgc
     4021 aggtctggca ttcggtcttg accgtctgac catgctgctg accggcaccg acaatatccg
     4081 tgacgttatc gccttcccga aaaccacggc ggcagcgtgt ctgatgactg aagcaccgag
     4141 ctttgctaac ccgactgcac tggctgagct gagcattcag gttgtgaaga aggacgagaa
     4201 taactgatat gactcaaata cacgaaatca ttcgcgttgc atcgaggcgg caactgagtg
     4261 aactcccatg agcatagata actatgtgaa tgggatgagc gaaggcagtc aacgaagagg
     4321 cagcgtgaag gataaagtgt ataagcgtcc cgtttcgatc ttagtggtca tctatgcaca
     4381 agatacgaaa cgggtgctga tgttgcagcg gcgtgacgat cccgatttct ggcagtcggt
     4441 aaccggcagc gtggaagagg gtgaaaccgc gccgcaagct gccatgcgcg aagtaaagga
     4501 agaggtcacc attgatgttg tcgctgaaca actgacctta attgactgtc agcgcacggt
     4561 agagtttgaa attttttcac atttacgtca tcgctatgcg ccgggcgtga cgcgtaatac
     4621 ggaatcatgg ttctgtcttg cgctcccgca cgagcgccag atcgttttca ctgaacatct
     4681 ggcttacaag tggcttgacg cgcctgctgc tgcggcgctc actaagtcct ggagcaaccg
     4741 gcaggcgatt gaacagtttg taattaacgc tgcctgaaca ggcagggcta tctggagata
     4801 tttttatggc aggtcatagt aaatgggcca acaccagaca tcgtaaagct gcgcaggatg
     4861 ctaagcgcgg taaaatcttc actaaaatca ttcgtgagct ggtaaccgcg gctaagctgg
     4921 gcggtggtga tccggacgct aacccgcgtc tgcgtgcagc aattgataaa gcactgtcta
     4981 acaacatgac ccgtgacact ctgaaccgcg caattgcacg aggtgtgggc ggtgatgatg
     5041 atgcaaacat ggaaaccatc atctacgaag gttacggtcc tggcggcacg gcaatcatga
     5101 ttgaatgtct gtctgacaac cgcaaccgta ccgttgctga agtgcgccat gcattcagta
     5161 aatgtggcgg taacctcggt actgatggtt ccgtagctta tctgttcagc aaaaaaggcg
     5221 tgatctcctt cgagaaaggc gatgaagaca ccatcatgga agcagccctg gaagccggtg
     5281 ctgaagacgt tgtgacctat gatgacggcg cgattgatgt ctacactgca tgggaagaaa
     5341 tgggcaaagt acgcgacgct ctggaagcgg caggtctgaa agcagacagc gcggaagttt
     5401 ccatgatccc gtccaccaaa gctgatatgg atgcagaaac cgcaccgaaa ctgatgcgtc
     5461 tgatcgatat gctggaagat tgcgacgacg tgcaggaagt ttaccataac ggtgaaatct
     5521 ctgatgaggt tgcagcgact ctctgatgag gcctgctaaa cagcaaaacg gagacgcgtg
     5581 atggctatta ttctcggcat tgacccgggt tcgcgcgtga ccggctatgg cgtcatccgc
     5641 caggtaggca ggcaactgtc ctacctgggt agcggatgca tacgcaccaa agtggatgat
     5701 ttaccgtctc gtctgaagct catctatgcg ggcgtgacgg aaatcatcac ccagttccag
     5761 cctgattatt tcgccattga acaagtcttt atggcgaaga acgctgactc agccctgaaa
     5821 ctgggtcagg cgcgcggcgt ggcgattgtg gcagcggtga atcaggaatt gccggtattt
     5881 gaatatgcgg cacgtcaggt aaagcaaacg gtggtaggta ttggtagtgc cgaaaaaagc
     5941 caggtgcagc atatggtccg caccttgctg aaactgcccg ctaatccaca ggcggatgct
     6001 gccgatgcat tagctatcgc gattacccat tgtcacgtca gtcagaatgc gatgcagatg
     6061 agcgaatcgc ggctgaacct ggcgagaggg cgactgcgtt aagttatacc gcctcggtca
     6121 gttccggctg aggcgtttcc actccctccg catgaatcaa caccaaaccc ggatgatgcc
     6181 acaaactggc tggcgtgacg gttttacgct cccacggaat agaacctaag aaccagaatt
     6241 tccagaaagt gagttttgca ttcggattgc tatttaacaa ttctccaaac gtctctattt
     6301 cacccaccgg aatacatagc gcttctttat aaatatcaaa aacaaatttt gaacagaact
     6361 ggcgcgaaga ttcgtattta aaaccggtgt ggtaaagttt gcgtagccgg gagggaacct
     6421 gttcaacaat tcgttgtttt tgttgttctg tcagcccagc gtctaatcgc tttatagcat
     6481 agcgttgatt agacgaacgt ttaataaaac gagatagcgt ggtgatggtt gaaaggggaa
     6541 cccggctttc tgcaaccagg aagtcttcac cgttatgacc gataatgatc ccgacgtgat
     6601 tactccagca atttgatgca gctgatattt gaccaaataa ggtagcacct atacatgtaa
     6661 agacgatatc accaatttca tattcagcag gataattaac attcacgttg
//
LOCUS       DAZUCC010000236         6649 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZUCC010000236 DAZUCC010000000
VERSION     DAZUCC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.237"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(43..246)
                     /locus_tag="ACHY09_004408"
     CDS             complement(43..246)
                     /locus_tag="ACHY09_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887287.1"
                     /translation="MPELPHFAPAPVPCPASALASQGGVELLTFGRLYRLTPGFGGFT
                     PLPYTAWSAAFHVAGAAYHGDDI"
     gene            631..993
                     /locus_tag="ACHY09_004409"
                     /pseudo
     CDS             631..993
                     /locus_tag="ACHY09_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            997..1352
                     /locus_tag="ACHY09_004410"
                     /pseudo
     CDS             997..1352
                     /locus_tag="ACHY09_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            1634..2773
                     /locus_tag="ACHY09_004411"
     CDS             1634..2773
                     /locus_tag="ACHY09_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HFS3887288.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            2893..4161
                     /locus_tag="ACHY09_004412"
     CDS             2893..4161
                     /locus_tag="ACHY09_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFS3887289.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEMISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            4169..5314
                     /locus_tag="ACHY09_004413"
     CDS             4169..5314
                     /locus_tag="ACHY09_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS3887290.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            5782..5928
                     /locus_tag="ACHY09_004414"
     CDS             5782..5928
                     /locus_tag="ACHY09_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887291.1"
                     /translation="MAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYR
                     GMSM"
     gene            complement(5999..>6649)
                     /locus_tag="ACHY09_004415"
     CDS             complement(5999..>6649)
                     /locus_tag="ACHY09_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS3887292.1"
                     /translation="VGSMGGVAPVEFIVRMANRFDGYAYIDDAHGMSIYGKNGSGYVM
                     SCLDNKLDDRIIIAGSLSKAFGSHGGFIACNYPDTINFIKTYGTTYAFGGPPSLPGIA
                     ACIAAADLHLDGTVSLRQNDLQQVIKYFDDKFNETEVINKGTTMPIRGLLIGNEEKAI
                     TICKKMHDRGFATTVAMYPTVEEGKAILRCAISAIHTRENIDSLHKNFIECINEQG"
BASE COUNT         1675 a         1682 c         1669 g         1623 t
ORIGIN      
        1 atcacctcgt cattaccacg tatatacaca gtatataatt attcagatat cgtcaccatg
       61 atatgccgcg ccagcaacat ggaaggcggc actccatgct gtatatggca gaggagtaaa
      121 accgccgaat cccggcgtaa gccggtacag tcgtccgaat gtcagtagtt ctactccccc
      181 ctgcgaggcc agtgctgacg cgggacatgg aacgggtgct ggagcgaagt ggggaagctc
      241 cggtatactg gccaaaccta cacgcctcct tccgtctccg ggaagatgct ggcttcgtac
      301 cctgcgtgcg tctgatctgt agctggccgt ggcacggcta cggacagatt ctgctccgca
      361 acagtatcgg cgtgcctgac ctcctgaaag tcccgtaacg cattttctca cctcccatgc
      421 cctgacgcta ttgagcgcgt tacagaacaa tttaagtcat ttaacgagcc tccaacggct
      481 cgatcgaaat aaatcaaatc gtaatcgtca acgatatatg agaggagacc ccgctcgccg
      541 caaatcgatg accgaacgta gtgagcgaat cgacgaggaa gcggaagaga acctgtacga
      601 aaatggagca attacgcccc accgtcaaga aatgagctgg ctctccacgg cgcgccatca
      661 tagacagatg tggctctgca actgcgccat gcgatcggag accgaccagt aattatcttt
      721 aatgcccggt tcgacacccg cattctgaaa cagactgctg ccgcacatag cgatccggct
      781 gactggctgg aagaactgac agtatattgt gcgatggaac tggcagcagg atattatggg
      841 gccaccaacc gctatggcac gatttctctg gcctgtgctg ccagcaggcc ggactgacat
      901 gggaagggct ggcacactca gctgatcgcc gacacacgga tgacgtcagg ggtggtaaac
      961 gctattactg catatcatca ggaattgctg cagcttatgt actgcgataa ccctgaactc
     1021 aactatgcaa acctgaaatc cctgcaaaaa cagctggatg aaatgtatac ctgcatccgt
     1081 aataatcaga tgatgctccc aaactacgga aaaatgcgcc ggtatggtga accggtttca
     1141 acaacgtttg tagagtcgac aatcaacgaa gtgatcgcca gacgatgacc caaaagcaac
     1201 agatgcagtg gggtagggaa ggtacgcatt atttgttaca gaccagaacc gctgtcctga
     1261 ataattaact gcaggacaaa ttcgccagtt ggtacccggg ttgcacgata gacgctcaga
     1321 gtggaggaaa gttatccgcc atggcagcat aactacttcc ccagtttttt cagctctccc
     1381 ttaccaatat cctacgacga tcacatcgaa aaaaaaacac ggaacaggcg aacagaaata
     1441 taattaatta tttgagttta aaggaaaaaa gaggatacaa gctttttgtt gtatttttat
     1501 ttgtataaac cgacaaaaaa gaggccatac ttcccgcttt ctttgtctgt ttacacggaa
     1561 catttcacca ctggatacgc taatagatcg gatatgagca catattgcgc ccgcctttca
     1621 tgcagagcat aaaatgaaca agattcacgc taattattac ctgcaagatt ctaccgcacg
     1681 ccagatagtt caacgcacaa tggggattat tccgtactcg gtaaatgtga tgaacgagca
     1741 tgggatgatc atcgcttccg gggaagcctc acgtttacat caacgccatg aaggtgcggt
     1801 actcgcattg accgaaaacc gggtcgtcga aattgattcc gcgaccgcca ataagcttaa
     1861 aggcgttaag cccggaatca atttaccgat taattttcgc gaccagctga ttggtgtact
     1921 gggcattacc ggcgagcctg gtgaagtgaa ggcatatgca cagttggtga agatggcttc
     1981 ggaattaatc attgaacaaa tggctctcct cgagcaaaaa cagtgggata agcgttaccg
     2041 cgaagagctg attaatcagc tgattttacg cgatccgcca ccggactcac tgaatgcggt
     2101 ggtctcatgg ttgggaatta atctccagct tccgcgggtg gtggtgatta tggaactgca
     2161 acaacctgat cgcgacgcac tgcataatct gatggagtat tttgaatatc gcgcccgcga
     2221 ccatttggtc acgttttccg actttaatga tctgataatc ttaaaaccta tctcgctaaa
     2281 aaacggcgtc tgggataaat cgttggaatt aagcgatctg cagagactta aacattacgc
     2341 ccagtcctgt ggtttttccc gactgattgt cggcggctat ttctctggag aaaacggcct
     2401 gcatcgctct tttcagaccg cgaaatccac ccagtccatg gcgaaaagac tgaagttgaa
     2461 gcatcagttc atttttcatg acgactatgc gctggcatca ttattagatg ggctttctgg
     2521 tacctggcaa gcggaagaat tatccaaaat ctggcgcaat ctggttgcca aggacaccaa
     2581 aggtatgctg caaaaaacgc tgcagaatta ttttgatcat aattgtgatc tttcgcaaac
     2641 agcgactggc ttgcatattc atgtcaatac attacgctat cgactgcaga gagtggaaga
     2701 tattacagga atacaaatca ataacttaaa gcagttattc tggctgtata tcggcatgga
     2761 gttacaacgc tgaactgtat ttccctccaa atttttcgcc gtctgacgtc gtatttttag
     2821 ccgatacaca caagtctaac gcggctgctt tctccatact gccccctgtt attttcatgg
     2881 agaaagcaga agatggtttc aataacagca actggcgcca tcatcgcact cgtggtggcg
     2941 atcgttttaa ttttacgcaa agtctcgccg gcttacggca tgattgtcgg tgcgctggta
     3001 ggcggtctgg tcggcggcgc cgatctggtg caaaccgtga cgctaatggt cactggcgcg
     3061 caaggcatta ccaatgccgt gctgcgcatt ctcgcagcag gtgtactggc cggcgtgttg
     3121 atagaatccg gcgcggcaaa caccatcgct gaaactgtgg tgaaaaaagt gggggaaagc
     3181 cgggcgcttt tagccttagc tattgcgacg atgattctga ccgccgtcgg agtatttatc
     3241 gacgtggcgg ttatcactgt tgcgccgatt gccctgtcta ttgcccgtcg tgccggtttg
     3301 tcgaaactcg ccattctgct ggcgatgatt ggcggtggta aagcgggtaa tgttatgtcg
     3361 cctaacccca atgctatcgc cgtttcggac acctttcatg ttcccctcac ctccgtgatg
     3421 atgaccggga taatccccgg tctgttcggc ctgatcgtgg cttatttcct ggcaaaatgt
     3481 ctcagccaga aaggcctgat ggtgatgccc gacgaaatga tttcacacga caaccacggc
     3541 aagcagccgg gatttcttgc cgctatcagc gcgccgctcg tcgccattgt gctgctggcg
     3601 ctacgacctg ttgcgggtat cgtcatcgat ccgctgattg ccctgcccgt cggtgggtta
     3661 gccggtgcga tccttatggg caggtttaag caaagtaacg cctttatgat gtccgggctt
     3721 acgcgcatgg cgccggtagc catcatgctg ctcggtaccg gtacgctcgc cgggattatc
     3781 gccaactcgg aactgaaaga tgtgctcatt catggactga cctcttccgg tctgccttct
     3841 tatcttctgg cccctgtttc cggcgcgatc atgtccatgg caacagcctc caccacggcg
     3901 ggaaccgctg ttgccgccgg ggtattcagc cagacgctgc tggggcttgg cgtatcagcc
     3961 cttgccggtt ccgccatgat tcatgctggc gcaaccgtac ttgatcacct gcctcacggc
     4021 agcttcttcc acgccacggg cggcagtgtc ggcatgctga tccatgaacg gttaaaactg
     4081 ctgccttatg aaacccttgt cggttttgtg gtcgccctga tttccacact gatgtttggc
     4141 gtcttcggac ttgccgggta aagacacgat gaaaatagtc attgcaccag attcatttaa
     4201 agaaagttta tcggccatgc aggtggccac cgctattgag cagggatttc gtgaaattta
     4261 tcccgaggcc gagtatgtca aattgccgat ggctgacggc ggtgagggta ccgtcgaatc
     4321 aatggttgaa gcaaccgaag gccattatct gttccagaat gtcaccggac cgctggggac
     4381 gccagtacag gcgcgctggg gtatgctggg agatgggaaa acggcgatcg ttgaaatggc
     4441 ctccgcatcc ggactacatc atgttcctcc agaatgccgt aaccctatgc ttaccaccag
     4501 ttatggcaca ggcgaactga tccttgccgc actggaccac ggcgttgagc gaattattct
     4561 cgggattggc ggtagcgcca ccaatgacgg cggcgcaggt atgctacagg cacttggtgt
     4621 ggcgatgctt gatacaaacg ggaaatcgct tcctttcggc ggcggcgcgc taagcaacct
     4681 ggcgctcatc gatgcctcag gctgcgacgc tcgactgaaa aacgtttctg tcaccgtcgc
     4741 ctgcgacgtt aacaatccac tctgtggcgc aacaggagcg tcagccatct tcggcccgca
     4801 aaaaggagca acaccagaaa tggttgcctc cttagaagct gcgctgacca acttcggtat
     4861 acgacttcag gacgcaaccg gacgaaacgt gatgacgacg cctggcgcag gtgccgctgg
     4921 cggcatgggc gcggcgctgt tagggattct gaatgcggag ttaaagccgg gagtggagat
     4981 tgttattgac gcgctgcatc tcgatagcct ggtcgcggat gccagcctgg taatcaccgg
     5041 tgaaggtcgt cttgatagcc agagcatcag tggtaaaaca ccgattggtg tcgcacgctg
     5101 cgctaagcgg tatcagaaac cggtgatcgc actggcgggc ggcttaacga ccgaccacca
     5161 gatagtgcat gagcacgggc tggacgcggt gttctccgtg ctgacgcaaa tcgccactct
     5221 gccggaagcg ctacaggatg cggaatacaa cctccgcgtt tcggcacgca atattgccag
     5281 cgtctggaaa ttatcacagc agcagatgcg ctaatcttca gtcaggccgg caagcagtag
     5341 ctggcctgga tattctttct caccaggccc atgctaaaac agcctgcttc agtcatccca
     5401 cgcaaggcac tagcatggca aaaaaagagg ggagtagtgg atcccaaaag acggttaagc
     5461 ggttaaattg aatggtcaat aagggggctg tttgggaaac gaatctatgg tcactcccgt
     5521 ttttgcaaca ccgattttga cgataagtgg gcttgcttga atctatccgg cgtctgaatg
     5581 ggattttatt cccgcgcctc gatgagttcc gcgcctgatg aacctcaaga aaatatacgg
     5641 cttcaatgag cctttccgtt tcacaggttc ctcaacaggc cggtgggccg ttcatatcat
     5701 caataccgtg atgctgatac tccgtaccca ccggaaccgc acctgatgcg gttccggctt
     5761 aagggctgtc aggactcgct tttggctgcc atcaacaaac cggaagagat gccggattat
     5821 ctcttccgtc aactcggttt taatcagacc tggcatgagt ggaagcggga cgaacagcac
     5881 aggcaacaac aacgtcggcc cggacactac cggggcatga gtatgtaatg tcagggacag
     5941 ctatacgcca taaaaaaatg aaatagatgg cctctctata aagaggccat gatatttact
     6001 aaccctgttc gttaatacat tctataaagt ttttatgtag gctatctata ttttctcggg
     6061 tatgaatagc tgatattgca caacgtagaa ttgcctttcc ctcttccact gtaggataca
     6121 tagcaacggt tgtagcaaaa ccacgatcat gcattttttt gcatatcgtt atagcttttt
     6181 cttcattgcc aattaacaat ccacgtattg gcattgtagt ccctttgttt atgacttcgg
     6241 tttcattaaa tttgtcatca aagtatttta taacttgttg taagtcattt tgacgcaagg
     6301 atactgtgcc atcgaggtgt aaatctgctg ctgcgataca tgcagcaatt cctggtaatg
     6361 atggtggacc accaaaagca taggtagttc catacgtttt tataaagttt atggtgtcag
     6421 gataattgca tgcaataaat ccaccatgag agccaaacgc tttagataat gaaccagcga
     6481 taataattct gtcatcaagt ttgttatcca gacaagacat tacatatccg gagccatttt
     6541 tcccatatat tgacatgcca tgagcatcat caatgtaagc atacccatca aatctgttag
     6601 ccattctaac aataaattcg acaggagcca ccccacccat cgaaccgac
//
LOCUS       DAZUCC010000237         6549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZUCC010000237 DAZUCC010000000
VERSION     DAZUCC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.238"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..983
                     /locus_tag="ACHY09_004416"
     CDS             36..983
                     /locus_tag="ACHY09_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS3887293.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENHFNLIIKNNVEHLVKSEST
                     VLRESICAVIAGKDCYQADTLSRLPGVKIISRFGTGIDNIDLHTARQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(1285..2661)
                     /locus_tag="ACHY09_004417"
     CDS             complement(1285..2661)
                     /locus_tag="ACHY09_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFS3887294.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LSGQFRNAALRLERLAPQGAETIITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(2661..3089)
                     /gene="tssE"
                     /locus_tag="ACHY09_004418"
     CDS             complement(2661..3089)
                     /gene="tssE"
                     /locus_tag="ACHY09_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFS3887295.1"
                     /translation="MATPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHSLMRQIEETLLRYEPRLNQIQVELLPQPGPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(3093..3629)
                     /gene="tssJ"
                     /locus_tag="ACHY09_004419"
     CDS             complement(3093..3629)
                     /gene="tssJ"
                     /locus_tag="ACHY09_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFS3887296.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(3610..4689)
                     /gene="tssG"
                     /locus_tag="ACHY09_004420"
     CDS             complement(3610..4689)
                     /gene="tssG"
                     /locus_tag="ACHY09_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFS3887297.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(4653..6413)
                     /gene="tssF"
                     /locus_tag="ACHY09_004421"
     CDS             complement(4653..6413)
                     /gene="tssF"
                     /locus_tag="ACHY09_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS3887298.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSNFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQERRVLDAHFS
                     PMGFDDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLENVIWPEGMSGFEIDV
                     VLSENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDSDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLSAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSTGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1526 a         1732 c         1739 g         1552 t
ORIGIN      
        1 ttttggggtg gcgttcattg atgtgagtgt cagctatgaa aaacgttctg gtcaccgttc
       61 ccagcttttc agcgcgttgt gtttctgcca gcaagctgct aagagaaaat cattttaacc
      121 ttattatcaa gaataatgtg gagcatctgg ttaaatcaga atccacagta ttacgagaat
      181 ctatttgcgc agtcattgcc ggaaaagatt gttatcaagc ggatacgtta tcgcgactac
      241 caggagtgaa aataatatcg agattcggga cagggattga taatattgat cttcatacgg
      301 ctcgacaatc aggtattgtg gtcaataatg ctgttgggat taattcaaat gcagtcgcgg
      361 aatttatcat cggtcttatt tttgcgagca tgagaaatat ccccggtagc tatcatgcaa
      421 tgcaaaatgg ttattggggt gagtcgcatg gctgcgagtt acaaggtaaa cgtattggcc
      481 tgcttggcta cggcaatatt ggcaaaacac tggcgaaaag gttatctggt tttgatgttg
      541 agcttttagc ttttgacaaa caacccgact atcaggttgc tgacaaagct ggagttcagt
      601 ttgtatcaat tgaagatatc tttatgcagt cgcatgtcat cattgttctt ttgccttttt
      661 cttctgagct ggagaacttc attagtcata aatatttgtc gatgatgcgc aatggtgctc
      721 tgatcatcaa tgccgcaagg ggaaagttgc tggatgaggg cgctttactt caggtgattg
      781 aagaacgaaa tgtgtttgcg gcgctggatg tgtttagctc tgaaccgttg gcgcaattta
      841 gcccactgct acatgccaaa aatatcatta caacgccgca tatcgcggcc gccaccgttg
      901 agtcatacca gcaaaccggg atacacgttg cccagtcgat aattgattac ttcgcaggaa
      961 gggagataaa aaacgtgttg tgagttcagc gaacaaagat tttaaataca aaaaaaccgc
     1021 aatttgtaga acacattgcg gtttgtcagc attctaataa attggttgcg ggggccacag
     1081 tcaagaggtt aatccaatct tttgaaactt aaggtattca ctgttcccga acccaagcta
     1141 tctaccgaaa tatacactca ttatctgatg tgaatcaacg agtagatcga atggcctgtt
     1201 cctctctttt tttgtacaca gagactccat tttaaaaaat ggcaaggtca catatccctt
     1261 attggtacat aaatcccccg atgtttactg actttgttcc accttcgtat ccagtaaaat
     1321 gacatagcga ttcaacaact gatttaaccg ggtatcaatt tgcgtcaaca gcgttggcga
     1381 aacctctccg gtctgttttt gctcttccag ttgccgaagc agttcttcca gcggcggctc
     1441 ctttaacgac tgccggatgc cgtacaccgc cgtttttagt tccgaaccgg tcagatatct
     1501 ccccttacgt tcatccagtg catccagctg cgccgacagg cgctgtagct ggctctgcgc
     1561 ctgagcatag ccattcagtg cctcagcaga cagggcgctc agctcccgct gtttgcgcca
     1621 ctgcgcgttc agcgccctga cctgaggatt atccggccac agggcatcaa gctggcgtag
     1681 ctggcgatat ccctgctcca gtggccagcg ggcaggcaat ttgtttaact gctccagttg
     1741 catctgactt gccgcaattg ttctgtcctg agccagcagc gccttatcct ttacatccag
     1801 ctttgccagt tcgccgctct ccagcgactg cggcaacggc atgacagaca gttgtattcg
     1861 ctggattagc gcgtccggct gattaagctg cgaccagccc cataatcctg ttccgccgag
     1921 accggccatc acaaccattc cggccataaa tatcggccat gtccgctgcc gggagatttc
     1981 aggggatggc acgggtggac tggcatcctt atcgttttgc tgaacggatc tggttgcaaa
     2041 aaccggctgt ggactttcct ctgacttttt cggttgattc atttcccggc ggggtaattc
     2101 agggggagtg ataatggttt ccgctccctg aggcgccagc cgttccagac gcagagccgc
     2161 attgcgaaac tgcccggaca atacgtccag cttcgtcata tgccacagtt cacactgttg
     2221 cagcgtggtt tcgatggcgg aaagatgctg tacacaacgg tagatctgcg gcagatcctg
     2281 atactgaata tccagcgttc tcagtgcctg ctgcattttt tctgtgaccc agctgagcag
     2341 tgatattctg gaatgtacct gtaccggcca gaagtcagcc cagcgctgga gcagtatatc
     2401 cagtgaaccg agtccgtctg ccatccctgc cagcccctgc cgtctggtta ttgccagcac
     2461 gtaacagacc agcgtctgta gttccacgcc attttgccgg aacagcgcga ggcagagttt
     2521 ttccacctga cgccagtcca catccggtcg ggccgggtga gtcagtttac tcatttcagc
     2581 ctgcagttca ccggattcct tataagtaca gggatcgccg cccatgacga actggctgat
     2641 aaaattcgcg ttacttgcca tcagtacgcc cgctcctgtt gtttgagatg acgcagaaca
     2701 attcgtcctt ccggagaaaa tacgccatca aagcgtatcc agccggtatc gggaagctgc
     2761 gcgtggatca ggtaattaag atgccccgga ccgggctggg gcagtaactc cacctgtatc
     2821 tgattcaggc gtggttcata acgcagcagc gtttcttcaa tctggcgcat caggctatga
     2881 atacccgcag ccatcccctg atggatcagg ctgagatccg gcagaccgta atccggcaaa
     2941 tgcgccagtg tgcctgcccg acagttgata atgcgttgca tattgtccat cacggaaagg
     3001 ataagctgat cccgctcact gacctgttcc agcggtaact cgccgctgaa actgaacgtc
     3061 agcatttcat ataaggaagg tgtcgccatc ggttatttat ccttgagagg cagcagtcgc
     3121 aggtcgcttc tcatcagttc aatcgagcgc ggcttgtcgg cctccagttc gtcccttttg
     3181 atcaccagac gccagctgtc gcttttttca tccgggtgat aaaactgccc aataatggcg
     3241 acaaactgcg tctctttatc cagcggcaca ttcagggatg ccgtgccttc aggttttacc
     3301 cagacggtgt gttttgccag tacgtccttc tccagcgtct tctcttcctg cataaacagg
     3361 gtgtcataat ccgccttatc aaaatcttcg cgggttttca gttgccagac atgcaccatc
     3421 gtggaaagcg gcgtatcttc cgcatccgta ttcagggcgc tgcgggagaa gaaatcgaga
     3481 tgcagtattt tgatttgttt gtagaaaacg gctgaggcca cgcttttcga ttcatccacc
     3541 actttctgaa tcagcccgca gccactcaga acaaccgtca gcatcgccat acagagcaac
     3601 gccggacgtt taaaacgaat aggaaacatg agtcatactc tccctgttac gaatggaaaa
     3661 agcgggtatt aatccctgat aacgccccag actgaccgtc actgttccgg tcgcgggcgc
     3721 ggcaaaggag gcgcgaagga tgccggtacg gctaatctgt acacgctgtt ttcccagccg
     3781 ggcagcaggc aacaggctga cgggcagcgt cagttgcaga cgggcctgac agcgccagcc
     3841 cagataaacg cgcagcagca ccagcaaatc gctgtgcagt tgcccgccgg gcagccagcc
     3901 acgtgcttct gcggcatctt cggtatacag tttcagcagc aactggctgt tgacgtcggt
     3961 accggtacgc cccagcagcg ttcgcgtttt caggctgatg cgtgaatttt ttgacagacc
     4021 cgcaggggcc gggagaatta caggctgcgg atcgtgaggc accaccgtcg ctttggtcag
     4081 cggggcaagc agccctacca gtgccgtgac gccttccgcc gtgcgcgtcg gcaggcgcat
     4141 cacgctgagc agggccagaa aacgggatac cggcgtggcg atatggttct cgctgccggg
     4201 aatacccagc ccgatgagtc ccagcagaca acgggaaatg tcatccacgg cacctgcttc
     4261 aaacgacgcc gggtagttat atttgcgcca gatgcggtag tactgagtaa taaagcgatg
     4321 gttaaaaata tcgagaaacg ccatcactgc gtcatgtccg tcatgacgct gggtaatgta
     4381 gtccagatag gcggttggca gcggcgaatc cacgccgtac aggccgagaa aagtggtgcg
     4441 taccgtgggg ggcgcgtcgg gattgcccac gtcacgttca acgtttttta actcgctgac
     4501 cggaaagccc atacctggat gaggacgaaa gcgcaccgca tcggtggcgg ggctgtcggt
     4561 actgcccagc ggcgcgttct gctggctctg ttccagcaac tggcaaaaac ggtaaaaatt
     4621 gatccgcgca atatccgggg ccagccggtc ggtcagcccg gtacgcgctg gctgtgatgc
     4681 tctgtccatt gcaggcactt tcctgtgggt tgcagaatta aggtcagttg agtgaataag
     4741 tggatatcgg catacagtgc aaagaagcgg tgcagcagtt cgccaaacag cgtgatatca
     4801 ccctctccgg caaagccggt gctgtccagc gtcacctgaa tatccacgcc acgcagcaga
     4861 aagcctcttt caaaacgctg tatcaggctg tgctgaacgt cgacaatcgc cgacagacgg
     4921 cggcggttca tctcgctttc ggtccagtcg tacagcgcca gcgtgccgcg cagaatttcg
     4981 gcgttatcca tcattgaaag aaagttagaa ccgaggtggc tgagcacccg ccagtggaag
     5041 cggtcacgcg ccgggggata acagggctgc gtcggggcgc acaggttgcg cacccgcagg
     5101 acgttctgtg aggcatgaac tggcgtatca agcagcgtgc tctgtaaagc cttacgggga
     5161 agctgtccgt tagtgccggt caggcttaaa gagagcgttt catcctccag catccggtcg
     5221 gaatcaaacg catcgccgcc cagaatcagc caggtatcat gcaggccgga agggccgcgc
     5281 ttaacccgcg tatggtaata gcgttccggt gcatcgtggc gcagcatccc gccccggtga
     5341 cgaaagctgg aaaacgggac atacgcctga ctgccggtat ggcgcgaaga aataatgttg
     5401 tcgacggaat aaatctcggt atgcccgtcc tgaatacgca tcgggcgcag cagaaattcg
     5461 ttttccagtg gcgaaaggtg taacggatcg gcttccagcg ggaacaggtt gatgaccggc
     5521 acgcagtgca gacggatatt atcgctgtca aacggcaggt cgtgaggcca gttctcgctc
     5581 agcaccacgt caatctcaaa gccgctcatc ccttccggcc agatgacgtt ctccagcccg
     5641 ttcagcgcca caaacataaa cttttcgcgg aaggtgaaat actccagcag caactggtag
     5701 ccgctgaacg ccgactcgcc ttttggccac agtcggtcat tgtcatcaaa acccatcggg
     5761 gaaaagtgcg cgtccagcac acggcgttcc tgccccggca gacgcagcca gaactgctgg
     5821 gtgccaagcg ttaaagcccg gtgcagcgca caggccaccg ggctgtcggc attgagatag
     5881 agcggcaaac gggatagatc gatctgtgac cagtcaccaa ccagcgggcc gcattcaaag
     5941 cgcagacgga taaccgagcg cccgtccggt tcatattgca atgacacgtc cggcagcgcc
     6001 agcggatgca gcgtgatatc ccgcgtagca ctgtagacac agcgggttct gcgctcgcca
     6061 acaggacgcg acaatacctg aaaattactc agtgtttcgc tctgcttcat ctgccggtga
     6121 tctgtcgaca gttcaaccac ggaaagtgac gggatggttc gcaggtagtg aggccacagc
     6181 aggctgacca gcccctcggt cagttcgggc aggtcgtcat ccagcttttc acgcaggcga
     6241 cccattaaaa aagcgaagcc ttcgaacagg cgctccacat agggatcgcg tgcgccgggt
     6301 ttatcaagat tgagcatcgc ggcgcggtcg gggtaagcct gcgcaaattc tttccccgct
     6361 tcacgcaggt agcgcatttc ggcttcgtag tagcgttggg ttagatcgtc catcggaggg
     6421 attatcttcc tttcattgaa agtgaatatc atgcaggtgt agaagtaaca tcatagttaa
     6481 atttattcta tgtgaatata ttatggatag ttgctgactt attgatttta ataatgatgt
     6541 attcttgtc
//
LOCUS       DAZUCC010000238         6519 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZUCC010000238 DAZUCC010000000
VERSION     DAZUCC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.239"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(175..2208)
                     /gene="betT"
                     /locus_tag="ACHY09_004422"
     CDS             complement(175..2208)
                     /gene="betT"
                     /locus_tag="ACHY09_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFS3887299.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2337..2924
                     /gene="betI"
                     /locus_tag="ACHY09_004423"
     CDS             2337..2924
                     /gene="betI"
                     /locus_tag="ACHY09_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFS3887300.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            2938..4410
                     /gene="betB"
                     /locus_tag="ACHY09_004424"
     CDS             2938..4410
                     /gene="betB"
                     /locus_tag="ACHY09_004424"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFS3887301.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFIPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRFIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4424..6112
                     /gene="betA"
                     /locus_tag="ACHY09_004425"
     CDS             4424..6112
                     /gene="betA"
                     /locus_tag="ACHY09_004425"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFS3887302.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GAECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGQEALPRSTARYFVANGMPVRAKKMSRDVN"
BASE COUNT         1591 a         1797 c         1788 g         1343 t
ORIGIN      
        1 attcacaaca gtccaaacgt aggttttggg cttcaatagt tcttttcttt tttattatct
       61 gttgcctggc aatcatcaat ctatactaag ggcttaactg tacagatata acgtatcaag
      121 aggtaagaga aaccgcgcca tatgcataca tggcgcggtt ttatgcaata acaatcacgc
      181 gtccgggaac atcacgctat ggcccggcgc ttcacgatgg agatgaataa agttaaggtg
      241 ccgctcgtac tggtcaagaa tatcggtgat cacctgctct ttgctgtagt ccatcaggtc
      301 gttgccctgg ctgccttcta acaggaaggt ttccagccgg tagtaggtcg atttaccgct
      361 acgtgcgcgg taggtaaagc ccggcaccga atattgctgc ggccaaatct gatagacaaa
      421 gttttgttct tcgcccatat gcaccaacaa atccagatgc cccaactgtt gtccctcttc
      481 cggcggcagg ctttttagct ccacgtatgc gccgcgcaac cgcagctcct gcgccacttc
      541 ttccattgcc gggtaacaga ccgtctccat catctgttta gtgtaacgcg tgcccggata
      601 attcatcagg cgcgagagac gttttttcca gctcaggcga tcctgaagcc ccagcggtcg
      661 cggcgcggta tcgcggttcg cactttcacg gcggtaatct tctaccttca gagatttata
      721 caaccccgcc atcacgaaga aaatcacaaa gctgaacggc agccccatga ttaccgtggt
      781 gttttgcagc gcggatatcc cgttggtcat cagcatgccg agcgtcagca ggccaatcgc
      841 caccgaccag aagacgcgca gccagccggg ggcgtcgctg ttgatatctt taagctgcga
      901 ggtgaaattc cccagcacca gcgcaccgga gtccgccgag gtcacataaa acagcaggcc
      961 agtaatggtg gcgacggagg cgctaaaggt aaacgccgga tattgcgcca gcaggctgta
     1021 gaagccgcgc tccggatgga ccatcgcttc ctcggcaaat gccgcgccgc cgtggatgat
     1081 ttcatacagc gcgctattgc cgaataccga gagccataac agcgtgaagg taaacggaat
     1141 aatcaacgtg cccagcacga actggcgaat ggtacgccca cgcgagatac gcgccaggaa
     1201 caagccgaca aacggcgacc atgccaccca ccatgcccag aagaagagcg tccagttatt
     1261 catccactca accggacggt cgaaggcaaa actgttgagc gtcatgccca taaagcgatt
     1321 cacatagtcg ccaacattca gcaccagtgc attaagcagg aacgaggtgt cgcccataaa
     1381 caacacgaac aggatcaatc ccagcgccag cgcaacgttg agttccgata acacgcgaat
     1441 gcccttatca acaccggatg tcaccgagat agtggcgatg atcaccgaca aggcgatcag
     1501 tgccgctttc gccgccatcg aatcgggaat atcaaacagt acgctcaagc catagttaag
     1561 ctgcaccaca ccgataccga gcgtagtggc aataccgaag atggtgccga tcaccgctgc
     1621 aatatccact gaatgaccaa tcggcccgtt aatccgttta ccgaagatcg ggtacagcgc
     1681 cgagcggatg gtgagcggca aattataacg ataactaaag tatccgagcg ccatgcccat
     1741 cagcgcatac atcgaccagc cggttaagcc gtagtgaaac agcgtccaga ccatcgcctg
     1801 acgcgcggcc tcaatcgtct gtcccgcgcc ttccggcggc tgcatatact gcgttaccgg
     1861 ttcggctacg gagaagaaca tcaggtcgat accgatcccg gcagcaaaca gcatcgccgc
     1921 ccaactcagc agactgaatt ccggtttgga ttgttctggc ccgagcttca ccgaaccaaa
     1981 acgcgaacaa gcgatacaga ccacaaagac aatatagagc gttgccgcca gcagatagta
     2041 ccaaccgaag gttttagaaa cccagtccag cgtgcggcca atccacaggg ccgagaagtc
     2101 gcgaaacagg atcgttgtca gggaaaacaa caaaatcagt ccggcggagg tgtaaaacac
     2161 caccggattg attttgtcct tttccctgct gtgtgaaagg tctgtcatcc agtatcccca
     2221 ctgttattgt taccatttaa aatcaaattc gtaacaatta agacacattt tatattgaac
     2281 gtccaatcaa taaccgcttt aatagataaa caacgctgat gaatggagtg gcgaaaatgc
     2341 ccaaattggg gatgcagtcg atccggcgca gacaactgat cgacgccacg ctggaagcaa
     2401 taaatgaagt gggcatgcac gatgcaacga tcgcgcagat cgcccgccgt gcaggcgttt
     2461 ctacggggat catcagccac tatttcaggg acaaaaatgg tctgctggaa gcaaccatgc
     2521 gcgatatcac cagtcagctg cgtgacgcgg ttttgaatcg attacatgca cttccgcagg
     2581 gcagtgcaga gctgcgatta caggcgattg ttggtggaaa cttcgatgaa acccaggtga
     2641 gcagtgcggc gatgaaagcc tggctggcct tctgggccag cagtatgcat cagccgatgc
     2701 tctatcgctt gcagcaggtc agtagccgcc gcttattgtc gaatctggtg agcgaatttc
     2761 gccgcgagtt accgcgccaa caggcacagg aagcgggcta cggccttgcc gcgctgattg
     2821 acggattatg gctgcgcgcg gcactgagcg gcaaagcgct ggataaacct ctcgcacact
     2881 cgctgacccg tcactttatt actcagcatt tacccaccga ttaaccgagg aaacgtgatg
     2941 tcccgaatgg cagaacagca gctttatata catggtggtt atacctccgc caccagcggt
     3001 cgcaccttcg agaccattaa cccggccaac ggtaacgtgc tggcgaccgt gcaggccgcc
     3061 gggcgtgagg atgtcgatcg cgccgtgaag agtgcccaac aagggcaaaa aatctgggcg
     3121 gcgatgaccg ctatggagcg ctcgcgtatt ctgcgtcggg ccgttgatat tctgcgtgaa
     3181 cgcaatgacg aactcgcaaa actggaaacc ctcgacaccg gaaaagcgta ttcggaaacc
     3241 tcaaccgtcg atatcgttac cggtgcggac gtgctggagt actacgccgg gctgatcccg
     3301 tcgctggaag gcagccagat cccattgcgt gagacgtcat ttgtttatac ccgccgcgaa
     3361 ccgctgggcg tagtggcagg gattggcgca tggaactacc cgatccagat tgccttgtgg
     3421 aaatccgccc cggcgctggc ggcaggcaac gcgatgattt tcaaaccgag cgaagtcacc
     3481 ccgcttaccg cgttaaagct ggccgaaatt tacagcgaag cgggcctgcc ggacggcgta
     3541 tttaacgtgc tgccgggcgt aggtgcggaa accgggcagt atctgaccga gcatccgggc
     3601 attgccaaag tgtcatttac cggcggtgtc gccagcggca aaaaagtgat ggctaactcg
     3661 gcggcttcct ccctgaaaga agtgaccatg gagctgggcg gtaaatcacc gctgatcgtt
     3721 ttcgacgatg cggatctcga tctcgccgct gatatcgcca tgatggcgaa cttcttcagc
     3781 tccggtcagg tgtgtaccaa cggcacccgc gtgtttattc cggcgaaatg caaagccgca
     3841 tttgaacaaa agattctggc gcgcgttgag cgcattcgcg cgggcgacgt tttcgatccg
     3901 caaactaact ttggcccgct ggtcagcttc ccgcatcgcg ataacgtgct gcgttacatc
     3961 gccaaaggca aagaggaagg cgcgcgcgta ctgtgcggcg gcgatgtact gaaaggcgat
     4021 ggcttcgata acggcgcatg ggttgcaccg accgtgttca ccgattgccg cgacgaaatg
     4081 accatcgtgc gcgaagagat cttcggtccg gtgatgtcgc ttctcactta tgaatctgag
     4141 gacgaagtga ttcgccgcgc caatgatacc gactacggcc tggcggcggg catcgtgacg
     4201 gcggacctga accgcgcgca tcgcttcatt catcagctgg aagcgggtat ttgctggatc
     4261 aacacctggg gcgaatcccc tgcagagatg cccgttggcg gctacaaaca ctccggcatt
     4321 ggtcgcgaga acggcgtgat gacgctccag agttacaccc aggtgaagtc catccaggtt
     4381 gagatggcta aattccagtc catattctaa ccaggaggtt tatttgcaat ttgactacat
     4441 cattattggt gccggctcag ctggcaacgt tctcgctacc cgtctgactg aagatccgaa
     4501 cacaaccgtg ctgctgcttg aagcaggcgg cccggactat cgctttgact tccgcaccca
     4561 gatgcccgcc gcgctggcgt tcccgttaca gggtaaacgc tataactggg cctatgagac
     4621 ggaaccggaa ccgtttatga acaaccgtcg catggagtgc ggacgcggta aggggcttgg
     4681 cgggtcatca ctgatcaacg gcatgtgtta catccgtggt aacgcgctgg atctcgataa
     4741 ctgggcgaaa gaacccggtc tggagaactg gagctacctc gactgtctgc cctactatcg
     4801 caaggccgag actcgcgatg tgggcgagaa cgactaccac ggcggtgatg gcccggtgag
     4861 cgtcaccacc tccaaacccg gcgtcaatcc gctgtttgaa gcgatgattg aagcgggcgt
     4921 gcaggcgggc tacccgcgca cggacgatct caacggctat cagcaggaag gttttggccc
     4981 gatggatcgc accgtcacgc cgcagggccg tcgcgccagc accgcgcgtg gctatctcga
     5041 tcaggccaaa tcgcgcccta acctaaccat tcgtactcac gccatgaccg atcacatcat
     5101 ttttgacggc aaacgcgcgg tgggcgtcga gtggctggaa ggcgacagca ccattccgac
     5161 ccgcgcggcg gcgaataaag aagtgctgtt atgtgcaggc gcgattgctt caccgcagat
     5221 cctgcaacgc tccggcgtcg gcaacgctga actgctggcg gagtttgata ttccgctggt
     5281 gcatgattta cccggcgtcg gcgaaaatct tcaggatcac ctggagatgt atctgcaata
     5341 tgagtgcaaa gaaccggttt ccctctaccc tgccctgcag tggtggaatc agccgaaaat
     5401 cggtgcggag tggctgtttg gcggcaccgg cgttggtgcc agcaaccact ttgaagcagg
     5461 cggatttatt cgcagccgtg aggaatttgc gtggccgaat attcagtacc atttcctgcc
     5521 agtggcgatt aactataacg gctcgaacgc agtgaaagag cacggcttcc agtgccacgt
     5581 cggatcgatg cgctcgccaa gccgtgggca tgtgcggatt aaatcccgcg acccgcacca
     5641 gcatccagcg attctgttta actacatgtc gcacgagcag gactggcagg agttccgcga
     5701 cgcaattcgc atcacccggg agatcatgca tcaaccggcg ctggatcagt atcgtggccg
     5761 cgaaatcagc cccggcgcgg aatgtcagac ggatgagcag ctcgatgagt tcgtgcgtaa
     5821 ccacgccgaa accgcgtttc atccgtgcgg tacctgcaaa atgggttatg acgagatggc
     5881 agtggttgac gccgaaggcc gcgtacacgg gctggaaggc ctgcgcgtgg tggatgcgtc
     5941 aattatgccg caaattatca ccggcaattt gaacgctacg acgattatga ttggcgagaa
     6001 aatggcggat atgattcgcg ggcaagaagc gttgccgagg agcacggcaa gatattttgt
     6061 ggcaaatggg atgccggtga gagcgaaaaa aatgagtcgt gatgtgaact aactcaggaa
     6121 cctatcagat tgagagttac cgttctacta tgaggaccga atccttatat aaaacactga
     6181 ggtaactctc atgcttcata tccgcagtcc cgccatcaaa ctttaagccg attcgctcaa
     6241 tctggcggat gatggtgcta tggatacgct gataaaccac aagtgtaatg ccactcagct
     6301 taagaatcac gcaaccagtg ctcctggcta gggttacttg ttctttccca ctcccggtta
     6361 gcagtggtca gcgatcaact gcgtaagccg ggcgtattta tcccaaagcg tggagtaata
     6421 tcaggtctac gctaaaaaag tcaatttatt cggttatcat gcacgaaaag gggacttgtg
     6481 gcgagtataa aaataagtca gccccaaaaa ctacgtgga
//
LOCUS       DAZUCC010000239         6479 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZUCC010000239 DAZUCC010000000
VERSION     DAZUCC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.240"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..255)
                     /locus_tag="ACHY09_004426"
     CDS             complement(<1..255)
                     /locus_tag="ACHY09_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS3887303.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQK"
     gene            468..1259
                     /locus_tag="ACHY09_004427"
     CDS             468..1259
                     /locus_tag="ACHY09_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFS3887304.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1269..2111
                     /locus_tag="ACHY09_004428"
     CDS             1269..2111
                     /locus_tag="ACHY09_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS3887305.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            2121..2897
                     /locus_tag="ACHY09_004429"
     CDS             2121..2897
                     /locus_tag="ACHY09_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFS3887306.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTVGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            2907..4448
                     /locus_tag="ACHY09_004430"
     CDS             2907..4448
                     /locus_tag="ACHY09_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase"
                     /protein_id="HFS3887307.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRKVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWMSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            4445..>6479
                     /locus_tag="ACHY09_004431"
     CDS             4445..>6479
                     /locus_tag="ACHY09_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFS3887308.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     VHNPWGLAIVIAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPHIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMEFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDYAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKN"
BASE COUNT         1560 a         1618 c         1742 g         1559 t
ORIGIN      
        1 tttctgtggc ggaagatctg agaaatcaag ggccttttct ttttgtcgaa tatcgttcca
       61 ggtatattcc agtacttcga taaataaatt ttctttatta ccaaagtggt gataaaccgt
      121 ctgtttactg caccccgcgg ccttgacgat attatcgact ctggcccctt cgtaaccata
      181 ttcggcaaat tcattaaccg cgcataacag cagcttttct ttcaggctct gtacgttgct
      241 ggtatcaaga tacataacgc ttccccacga cctcttcgtg gtgactatca taacctgatt
      301 tgatttcagc ttcagaaaaa agggcaaaaa cttgtacttt catcaggtta tcaaatgtcc
      361 tgtgatcgcc atcgcatatc aaccaactag atggttgatc tttttaccct ccagtcgcaa
      421 gctaatcatc actaaggaaa caaaattcaa aaaaagagag ctattgtatg aaatccactc
      481 gtcctgttgc cgtgattacc ggagccgctc gtggtattgg aaagggctgt gcgctggagc
      541 ttgcgcgtgg cggatttaat ctgctgatta atgatcgtcc ggatgcggat agcgtagaaa
      601 aattacatgc cacacaacaa gaatgtctgg cggaaggcgt ggaagtgatt tgcttccctg
      661 ctgatgtggg cgatctctcc ctgcatgaag agatgctcga cgcagcgcaa aaccagtggg
      721 gacgtctgga ctgcctgctc aacaacgcag gcatttcagt gaaaaaacgc ggtgatcttc
      781 tcgacctcga accagacagc ttcgaccaaa atattgccat taacacccgc gcaccgttct
      841 tcctcgctca ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcagaactgc
      901 cgcaccgctc gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg
      961 gcgaatatac cattgccaaa actgccgtct ctgccgctgc tcgtctattt gctgctcgcc
     1021 tttgtaatga acaaattggc gtatatgaag tgcgtcctgg tctcatcaaa accgatatga
     1081 ctattccggc aacggcctat tacgacgaac tgattgccaa aggtctggtg ccgtggggac
     1141 gttggggtta tccggctgat attgcttcca ctgtccgcgc gatggcggaa ggtaaactga
     1201 tttacacctg tggtcaggcg gtcgccatcg acggcggctt aagtatgccg cgcttctgag
     1261 gctgaatcat gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac
     1321 tgggctataa aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg
     1381 ccattgcccc ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc gcccgccagc
     1441 ttgctgccag taacataaac gtttccggtt tgtgccgtag cacctactac accgcaccga
     1501 cgctggcgga gcgcaagctg gcgattgatg ataaccgccg ggcgctggac gatgccgctg
     1561 tgctaaatgc cgcctgttac atgcaggtgg ttggcggtct gccaatggga acaaaagatc
     1621 tgtacgaagc acgggaacag gtgaaacaag ggattcgcca gttgctgcca cattcgaaag
     1681 aggtcggcgt gccaattgca ctggagccac tgcacccgat gactgctgcc gatcgttctt
     1741 gtctgtgcac gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggcgaat
     1801 ttggcctcgg cgtggcggta gatgtctacc atgtctggtg ggacccggat ctcgccagtc
     1861 agatcctgcg tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttagtgccga
     1921 ccaccgatct ggtcaatgac cgtggaatgc cgggagatgg tgtcatcaat attccgtcaa
     1981 ttcgtcggct ggttgaaaac gccggattta atggcgctat tgaactggaa atattctcac
     2041 cgtactggtg gcagaaagat attaacagca cgctggatat tagcgtcgat cgcatcgcac
     2101 attattgcta aggaataatc atgagtgacc aacccgttct gttcagcaga gcggcggctt
     2161 cttgccgcct gacattgaac cgtgaagata aatgtcatgc tattaatgaa gaaatgatcg
     2221 aatctctcga ccattattta aatgaaattg aaaacgacac cacattacga ttagtcgaat
     2281 taacggcaac cggcgataaa ttcttttgtg cgggcggcga tattaaatcg tggtcggcct
     2341 attcgccatt agatatgggc agaaaatgga ttaaacgcgg taacgaggtt tttaatcgcc
     2401 tgcgcaactt accccaatta accgttgcga atctcaacgg tcataccgtc ggtggcggta
     2461 ttgaacttgc cttgtgctgc gatattcgta tcgctcgccc cggcgcgaaa ttctctaacc
     2521 cggaagtgat gctcggcatg gtgcctggct ggatgggcat tgaacgcgtg cttaatcagg
     2581 ttggcccggt ggtcggtcgc cagatgctga tgctgggtaa acgcctgacg gcgcaggaag
     2641 cccaggtggc gaatttaatt gatgaagtgg tagaaaaaga acaggtggaa agctggatgg
     2701 cgaaccagtt agcgcagctg gaaaaatgtg gtccggtggc actggcacat attaagcaac
     2761 tgattctggc gctggaaaat aaacacgccg attactccca ccagttactg gctggattaa
     2821 tgtccgccac tcaggattgc cagcaggcga cgcacgcatt tgccgaaaaa gccggcgtga
     2881 gcttccacaa tcaataagga aattgtatgc gtaagttatc aaccgccgag acactggcgg
     2941 cgcaaattca ggacggcgca accattgcca ttagcggtaa cggcggcggt atggtggaag
     3001 ctgaccatat tatggccgcc attgaagcgc gtttcctgca aaccggacac ccgcgcgatc
     3061 tgacattaat ccactcgctg ggtattggcg atcgcgacag caaaggcact aaccgttttg
     3121 ctcacgccga aatgctcaaa cgcattattg ccggacattt tacctggtcg cccaagatgc
     3181 aggaactggt gaaaagcaat gctattgaag cctactgttt ccctggtggc gtaattcagg
     3241 cactgctgcg ggagatcggt gccggacgtc cggggctttt tacccacgtt gggctgggat
     3301 cgtttgttga tccacgcaat ggtggcggta agtcgaatga atgcacgacc gacgacctgg
     3361 tagaactgat tgaaatcgac ggtgaaacca aactccgcta tcgccctttc aaagtggact
     3421 acgcgatttt gcgcggcacc tatgccgatc cgcgtggcaa cgttagcctc gaagaagaag
     3481 cgattgatat ggatagctat tcgatggcgc tggcagcaca caacagtggc ggcaaagtgt
     3541 ttgtacaggt gcgcgatgta ctggaagctg gcgccattga acctcgcaag gtcaagctac
     3601 cggggattct ggtcgatggt atcgttgaac accgcgaaca accgcaaacc tatcttggcg
     3661 gttacgacct gacgatcagc ggtcaacatc gccgtctaag ttctaacgac gctattgaac
     3721 tggttagtca tccggtgcgt cgcctgattg cccgtcgggc agcacgggaa ctggtggcag
     3781 gcgcttcaac caactttggc tttggtattc cgggcggtat tcctggggtt gcgctgcgcg
     3841 aaggcgtgcc ttatcaaagt ttatggatga gcgtcgagca aggcgtacat aacggcatga
     3901 tgctggatga tgctctgttc ggctgcgccc gtaacgccga cgccattatt ccatcactcg
     3961 atcaattcga attctacagt ggcggcggta tcgatatcac cttcctcggc atgggagaga
     4021 tggatcggta cggtaacgtc aacgtatccc atctcaatgg caatctcatt ggccccggcg
     4081 gcttcctcga aattgcgcaa aacgcccgta aagtggtgtt ctgcggtacg tttgacgcca
     4141 aaggcagcaa gattgatgta acgcctgatg gcctgcatat cgcccagtca ggtcaaatcc
     4201 ctaaactggt tacccaagtg gaaaaaatca cttttagcgc cgcctacgca cagcaaagtg
     4261 gtcaggaagt gctgtatatc actgaacgcg cagtattcca gttaacggca gaaggcgttg
     4321 aattaattga aatcgcgccg ggtgtggaga ttgagcgcga cattctgccg tatatggctt
     4381 tccgtccaat tatcaagcac ccacgcctga tggaaagtag cctgtttacg ccgatggagg
     4441 atgcatgagt cagcttgatg acaccatcct tgatgcgctg acgcacgtta ctttcccaaa
     4501 gggttttgca caggcagaac ccgcatgggt tgtcacggta gacggtgttg attacccact
     4561 ttggcaaaca gatgccctgg tagtcggcag tggtgcagcc gggctgcgtg cagctgtgga
     4621 acttaaacgc cgccagcaaa atgtgttaat cgccaccgcc gggttataca tgggaacatc
     4681 ggcctgctcc ggttccgata aacagacgct gtttaccgcc gctaccgcgg gcaacggcga
     4741 caacttcacc aaactggcag aggcactggc gagtggtggg gcgatggatc acgacactgc
     4801 ttatgtcgaa gcggtaggtt ctctgcacac tcttggcggg ttgcaatatc ttggtctgga
     4861 attaccggaa gatcgctatg gtgcgattct tcgttatcaa accgaccatg acgaagccgg
     4921 gcgtgcaacc tcgtgtgggc cacgtacctc aaggttaatg gtgaaagtgc tgctggaaga
     4981 agtacagcgt cttgctatcc caatgttgac cagtgcaaca gtgattaaac tgctgcatca
     5041 gcgtgacgaa aacggcgaag accgcgtggc gggggctatc ctcgcgaccg gtcatcgcgt
     5101 ccataaccca tgggggctgg caattgtgat tgcgcccaat gtggtactgg caacaggagg
     5161 gcctggcgag ctttatcgcg acagtgtgta cccacacaaa tgtttcggct cgctggggct
     5221 ggcgctggaa gaaggtctga cgctaaccaa tttgactgaa agccagtttg gtataggcac
     5281 accgcgcagc acgtttccgt ggaatttgtc cggcacctat gtgcaggtga tcccacatat
     5341 ctattccgtg gatgccgggg gcaacgagta taacttcctc gcggattact atcgcaccac
     5401 ccaggagctg gcttcaaaca tttttcgtaa aggctatcag tggccgttcc acgccactcg
     5461 ggtaatggag tttggctcca gcttgttaga tatggctgta gcgcaagagc agcaatcagg
     5521 gcgtcaggta tttatggatt tcaatcgcaa tcctgaagcg gtgccgggtg acctgccctt
     5581 ctcattagat cgactggacg acgacgttcg cgcgtatctg gaaaataacg acgctctggc
     5641 accatcgccc atcgaacggc tgcaacgaat gaatccgctg tctatctcgc tgtataagat
     5701 gcacggttac gatctcacca cacagccttt gcagtttgcc atgaataatc agcatatgaa
     5761 tggcggcatt gaagtggata tctggggaca aacctccctg cccggctgtt tcgccgtggg
     5821 ggaagttgct ggcacacacg gcgtcactcg ccctggtggt gcggcattga atgccgggca
     5881 ggtttttgct gttcgtctgg cacgttttat tggttgcacg caaaaacgta atattgatgg
     5941 agatatagca cagctggtag ctccggcact ggcttctata agagagatca ttactcaggc
     6001 gcacgataac gggaccggga tgccgttgtc ggttgtgaga gaaaaaattc aggcacgaat
     6061 gtctgactat gcgggattta tttgccatgc cgataaagtc cgacgtgcca ctcgtgatgc
     6121 cctgctattg aacgaatttg tccaacggca tggattggca atcaaacatg tgggtgaagt
     6181 tgccgagctg tttatgtggc ggcatatggc gctgacttct gccgcggtat taacacaatt
     6241 aacgcattat attgatgctg gtggtggtag ccgtggggcg cgtatggtta ttgatccaca
     6301 aggcaaatgc ctaccacaaa ctcgtcgcgg cgcaaaagaa gaatggcgtt ttcgctctga
     6361 actcgctgaa gacaaaaatc acaaattaac aattcaatat tcgcaaggtt cttttattac
     6421 cgaagtaaag tctttacgta tgcaaccgtg tattaacggt atttactttg aaaaaaact
//
LOCUS       DAZUCC010000240         6464 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZUCC010000240 DAZUCC010000000
VERSION     DAZUCC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.241"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..3649)
                     /locus_tag="ACHY09_004432"
     CDS             complement(<1..3649)
                     /locus_tag="ACHY09_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFS3887309.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PLTEAIPSPPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGIMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLYFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTNDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGADGYYNATLDYSDGCTTVTDGKGIHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAVHGQLSRYTAADGAVWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAGTGAEGFRYDRHGNLLAWTDGNGVDWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYLLDNCGRVTEEHDWGGVTWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEIITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWGGLVPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQ
                     SAGRHRISEETE"
     gene            complement(3669..4130)
                     /locus_tag="ACHY09_004433"
     CDS             complement(3669..4130)
                     /locus_tag="ACHY09_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFS3887310.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRIDQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(4158..6059)
                     /locus_tag="ACHY09_004434"
     CDS             complement(4158..6059)
                     /locus_tag="ACHY09_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFS3887311.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVRPPLWRA
                     GLRQNFRIFQNEDIRSILGTILQENDVTEWSPLFSEPHPSREFCVQYGETDYDFLCRI
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDWPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTEHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPKPKPSVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPANQDSSCWIRVAQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HEDNRSPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVMIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKQIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1220 a         1936 c         1764 g         1544 t
ORIGIN      
        1 gctcagtctc ttcacttatc cggtgcctgc ccgcagactg cgccttcagg tagccgcagc
       61 tgtcatagcc ataagtctca tcgccttcgc catagcccag cccggtgcct gaggctgtca
      121 ccgccgtgac ctggccgttc cggtccagcc ggtactcccg gcgtgtttcc cgtccgtaat
      181 cttcccggct ggtgacacct gacacgttac ccgcgctgtc gtaacggtac tcccgtacca
      241 gtgacggaac cagcccgccc catgtggctt cttccggaaa aaatccggcg cgctggctga
      301 caggctggcc ggctgcattc cagccctgct cacaggcaaa accccggtta ctgtgccgga
      361 gcacttcacg tcctgcctca tcacggctga acgtcagcgt ctcatccccc ggcaggataa
      421 cctccgtcag ttctccggcg gcgttgtagc ggtaggtggt tttcagcagg gattcatcgg
      481 ggtgtctgtc gcgttcatct tccggtggca ggatacagcg gatgacggtg tgttcatcgg
      541 ggtaatggta ctcaatggcc cgtcggccct gagtggtgac attcacccgc ccgcgttcgt
      601 catagccggt gcgctgtgat gtgccgtccg ggctgaagat acccgtcagc ctgccggatt
      661 tgtcatacgc atactgcgtt ttgccttccg gcgtgatgat ttctgccagg cggcctgcgg
      721 cgtcccggct gtagaggatg gtttcctcca ggccgttaac cctggcggta cacaggccat
      781 cggcgtcata gcggtaacgc caggtcacgc cgccccagtc atgctcttcc gtcacccgcc
      841 cacagttgtc gagaagataa cggtatgact caccctgcgg gttgatgact tctgtcagtt
      901 gcagcgtgtc tttatcatag cggtactgcc agcggtggcc ttcaccgtcc gttcgcgcca
      961 ccggcaaatc aaacgggccg tattccattg tccagtccac accgttaccg tccgtccagg
     1021 ccagcaggtt gccgtggcgg tcatagcgga atccttctgc gccggttcct gcccgctcaa
     1081 ggccgctcag tttatggtgc tgctgccact gcaaccgcac tgactgaccg ccggggccgc
     1141 gcatcttcac cggcaggtcg tgaccactgt attcggtgtg gtgttcacgg cccatcacgt
     1201 cggtgagtgt ttccggccgg ttgtggtggt cgtactctat gcccagacgc cgttcatcct
     1261 ggcggaatat ccccgacagc agcccctgag cggtgtacgc cagccgggtc tcttcgccct
     1321 gtggcgatac cagactcacc ggcaggccgc gttcatcgca ctgctgtgtc cacgtctgcc
     1381 cggcagggtc ggtgatgttg ctgagcaggc cgcgctcatc atagcggtac aaccacaccg
     1441 cgccgtctgc cgccgtataa cggcttaact gaccgtgtac ggcgttgtat tcaaatttct
     1501 ccacccgtcc ggcaggggag tggcgggcca gcaggtgatg gaattcatcc cattcatacg
     1561 tggtggtgct gccgtccggc gcttcttccc gcagaatatt gccgtcagga tcatagtaat
     1621 aacggtgaat gcccttgccg tccgtcacgg tggtgcagcc gtcactatag tccagcgtgg
     1681 cgttgtaata gccgtccgcc ccggtgacat tcacgcaccg gccctgttca tcgtaggtga
     1741 aacggctcca cgtctggtcg ttatcggacc agcggatgat ccggtccgca gcgtcgtact
     1801 cataaaacag gtggtagtcc agccgcgcat ccgcttcggt cagccgcccc cgcgcatcct
     1861 gttcgtaggt cgccagcgtc tgtatgccgt tatccgtgcg gtgaatggct ttcaggtagc
     1921 cgctggcgtg gtacgtcagt ttaagccgga tcccgtcggt gtgactgact cccgtgatgc
     1981 ggtactggct gtcgcgctca aacgtgatgc ggttgctggc ggtgtcgctc atcccgccca
     2041 gcatgtacat ccggtcaccg gggctgtgtg ccaggcggtc atcattggta tcgtcagtca
     2101 ccgggtggcg caggcgcatt cccggaaagg cgtcataaaa gaagcggctg ctcagggcat
     2161 cacggtcgaa cagctccagg taatcgtcgc ggcggcgcag gatatactgg gcgtgatacg
     2221 ggttaaccgc cgtatcactg tctccgtcga aggcaaagta cagcgtggct ccctggctga
     2281 tgatatccac ccggtttccc tgttcacgca cccaggcgta ttcgctccag gagtcgctcc
     2341 agcccacgcc cgtcaggcca tgcagtggca gtacagaact gtggaaacgg gtgaaggaga
     2401 gggggagtgt ctggcccagc gtaaagtcgg tacgttcgtc gcagtatgcg ccggttaccg
     2461 gatcgacagg atctgttacc catctgatga ctttattggc aatacttttt aacgacttac
     2521 ctgttttccc cagcaacgcg ccgagctttg cccccagcag cttcccgctt ttccccagca
     2581 ggtttcccag tttgccgagt ttcgccagat tccccagttt tcccagcagt cccgcgggcg
     2641 gcagaagata gctggctatc gttattcccc ccaggatcat tctctgccac ggcgggaact
     2701 ccggctggat atccagatag gtcacctgtt caccgccaat aaagacgtcc ggcgaacctt
     2761 ccacgattgc cgcgctgcac tccagtttgt cgcccttgcg cgcggcaggc tgattgttga
     2821 taaagacgtt gctgctgccc tgggctatcc tcggtggttt gttctccttg tcgcagagcg
     2881 ccgtgtcctc accggcttcc gggaaatcca cgtctttttt gatttcgcca cgcccgccca
     2941 ccatggtggc aaactccatt aataccggac cggcgggaaa cagaagcgat gcgccggcgc
     3001 tgacgcgatc cataataccg gcattgctgt ccgtcagggt gataacagcg ttcccccagg
     3061 ccttcgccag tggcactgct ttttccgctg ccgattcaag caggtcagtg gcaatatccc
     3121 cccatgaagg ttccccctgt ggtggagagg gtattgcttc agtcagggga ggagggggaa
     3181 gggtgacggc ggccctggct gctggcttac cgttaatttt tacgttttca gaaccggtat
     3241 tgatggcgcc gtccgtgtca caaaaactgt ttatccatga ctcaagctgg ttctgtttct
     3301 cctccacata atcactcgcc ttatagctgg catacatcag cgcaggcacc agaaccagcg
     3361 cataagggcc ggtggcgcag gcggccataa tcaggcaacc ttctgcgata gttactatcg
     3421 cccccagcac ggcccctgcc agtgccccca gaaaactttt atgctgaatt tcatcgccca
     3481 gcctggcggc aggtgggccg gacttctttg ctgccagcat ctgtgcaatg gctttgtcgt
     3541 tggctgcccg tttcatctgg ctggcgacca tcgcctgctg tgacagcatt cgcattgcca
     3601 gcgtaccgcc tgcggtcgct ccctgtgggc cgcctggtcc ttcactcatg tgttatccct
     3661 cgtttatgtc agcgcggctg gtccttttgg atgtcattct ccggtggcaa cggggtgaag
     3721 ctctttatca cctccagcag catcgtcttt tgactgttct tcagggataa ggaggtgctg
     3781 aaagtgaagg tcagcagcgt atctccacgc cgctggaata cggctatctg gtcaatccgt
     3841 ccctggttcc cctcatcttc cggaccgttc caggaataat ccagcagcca ggccggggcc
     3901 tctgccagag tgatttcccg gcaggcatgt tcggtgtacc catcaagttt gccctggtac
     3961 agcgccagcg tttcctggta cagctctggt gtcagggttc cctgtggaac ggcttcgcgg
     4021 gtaatgacca ggttgatgcc actgttatct gtggacacca ggacctgcat tgactgatcc
     4081 tgccattgcg atggcagctg aatatgacct tcattaaaag tgaactgcat aaaatactcc
     4141 ctgataatct gaatgaatca gttgatgaaa acagaaccgc ccacgatggt gatgccatcc
     4201 ttcgacagcc tgattaccgt cgattccccg acctgtagct gaatgccatc cggcgtcagc
     4261 gcaattttgc tttcctccac ggccagcata atttgcttaa ttgccgtata agcctgtata
     4321 ccgtcgcctt cgccaccatt gtttacagcg tcaacatgca ctgaaccggc aatcgtgcct
     4381 ctgtcctgaa aaacatcctc agataaagac ccttttagta cgcctgtgat tttgtggcct
     4441 ttttccaccg taagaaggcg gttaccttcc tggatcatga ccgtctggtc attttttacg
     4501 gtttcgcggt gatcgtgttt tacggtggtg gtacggtcgt tgagtacctc ggtatccatg
     4561 ttcttctggg cgtggatgta gacctgttct ttccctgtcg catcgtcaaa cttcagctcg
     4621 ttaaacccgc tgcccatata tgttttggag cggatggtca tctgcgtctt cgttcccggc
     4681 aggctgcccg gcgagcggtt atcttcgtgg taggtgcgcc ccataatgat cggctggtcc
     4741 ggatcgccgt tgaggaagtc cacaatcacc tcctgaccca cgcgcggtat cgccaggttg
     4801 ccaaatcccg gccccgccca cgcctgcgcc acccgtatcc agcacgaact gtcctgattg
     4861 gccgggttat agcggtccca gttgaatttc acccgcaccc ggccatgctc gtcgcagaag
     4921 atttcctctc ccgcaggccc cgtcacgacg gcgctctgcg gaccgtccac tgacggcttt
     4981 ggcttcggct gaggtcgcca cgtccggtct gccggtatta ccgcaaagtg gttattcagc
     5041 gtggtacctg accccctgcg tcccggcacc gcctgcggct gttcgccgtg cagttcgctc
     5101 gccaccacct gccattcccg gttcaggttc gcctgcggat gctccgtcag cacaattcgc
     5161 cgtccaggcc atatctccgg cgagcggctc attccccgcg cggtttcggc gttgtttcgc
     5221 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgcccgggc
     5281 cagtcataca cttcatactg tgtgcgctgg taatcctgat gctggccttc ctggtcaaaa
     5341 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacaga ggaaggacgg
     5401 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
     5461 gggttccagg ggatttcaaa ggattccggc agatagcgga ccgtgtcgca cagcaccaga
     5521 ctctggtcgg tacttttctg cgcgtgctcc tcatagaaga agatgccttc ctccgctgct
     5581 atccggcaga gaaagtcgta atcggtttca ccgtactgga cacaaaactc acgggaagga
     5641 tgcggctcac tgaacagcgg gctccactcc gtgacgtcat tttcctgcag tatggtgccg
     5701 aggatgcttc tgatgtcctc gttctggaat atacggaagt tctgacgcag tcctgcccgc
     5761 cacagcggcg ggcgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
     5821 tcaaaccagg tcaccacgcc tttcacccgg cgctgtacgt catcgccctg ccataccgtc
     5881 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctgactgacc
     5941 agagaaaggt caagggaaaa aagtgaagag agtgactggg tgagatgaaa ggagaccacc
     6001 gcaaaagcgt ccggtggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
     6061 gcaacctccg tgaaaatgaa taataatccg catatattgc atgtacaaca tgtcgggtaa
     6121 tgagataagc gggccggctt caggcgttac cggggaatat cagaaaagtg tcatggttat
     6181 gtgatgattt tgtccgggat tttacttcag agaaacgggg ggctgttgac tcaacaaaat
     6241 aatcacaaac tcaactgacg ggttcacgat gttattaagg acaggctcgt atattacatc
     6301 gagtaacaaa aaaacacctg atatgcaaag tcaaaccaat atttgtaatg tctttttatt
     6361 cacaataata aataactcaa taaacattat tgaaatgaat gatggtttgt ataaggaata
     6421 tacgctaccg ccccaccctg ataacatgat ctgcacattg ctat
//
LOCUS       DAZUCC010000241         6412 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZUCC010000241 DAZUCC010000000
VERSION     DAZUCC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.242"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..526
                     /locus_tag="ACHY09_004435"
     CDS             <1..526
                     /locus_tag="ACHY09_004435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887312.1"
                     /translation="QKDYSPFSGNIYSVIIRSGISVITLWFFLFFFSSSMLRTPVTGL
                     YLTPFISSLRIDIHPQYITRPSVKVSSRINADKLVGNIEHFILCYPSYNTITLQSHIL
                     RHGIRNRIANNLKKKGILYKEKARKTPLCETIVLNLRYGGKTRYRLFSCRRHPNGFRV
                     HRNGGAFIIQTQNN"
     gene            complement(529..873)
                     /locus_tag="ACHY09_004436"
     CDS             complement(529..873)
                     /locus_tag="ACHY09_004436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887313.1"
                     /translation="MKAVEVYGRHSDVVMSLERQTDLLEAAGNVLKQRWFYRPWGSSK
                     ASVMLTGILVIPVFLVLSGLLSAGYPGLLCITVSGCYFSGIAAFSLRAKDPVGLCWSV
                     FSFILLYLLLKK"
     gene            complement(1085..2077)
                     /gene="traU"
                     /locus_tag="ACHY09_004437"
     CDS             complement(1085..2077)
                     /gene="traU"
                     /locus_tag="ACHY09_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFS3887314.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWNCIFP
                     LSLGSTKVSQGKVPDTINPSMPIQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGITIPELGKTGQGTAKKDDKQVNGAFYHVHWYKYPLTYWLSIITSAGCLMGGDMD
                     IGYISEIDPTWTDSSLTTILNPEAVIFANPIAQGACAVDAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNSIGVDVAVCYEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(2074..2706)
                     /gene="traW"
                     /locus_tag="ACHY09_004438"
     CDS             complement(2074..2706)
                     /gene="traW"
                     /locus_tag="ACHY09_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFS3887315.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEADMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            complement(2703..3089)
                     /gene="trbI"
                     /locus_tag="ACHY09_004439"
     CDS             complement(2703..3089)
                     /gene="trbI"
                     /locus_tag="ACHY09_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFS3887316.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEAQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(3086..5716)
                     /gene="traC"
                     /locus_tag="ACHY09_004440"
     CDS             complement(3086..5716)
                     /gene="traC"
                     /locus_tag="ACHY09_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFS3887317.1"
                     /translation="MSNNPLEVVTQAVNSLLTALKLPDESAQANQILGEMNFPQFSRL
                     LPYRDYNQESGLFMNDSTMGFMLEAIPINGANETIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGERIEYGLREFSWSGEQAERFNAITRAYYMKAAETLFPLPEGLNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLTRQNRARIDDVVDFLKNARDNDQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(5847..6194)
                     /locus_tag="ACHY09_004441"
     CDS             complement(5847..6194)
                     /locus_tag="ACHY09_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887318.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPVYIVYLAQQPATPEQVTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMEEARRVRENELNKIREKQ
                     LQALSDADSAKER"
     gene            complement(6222..>6412)
                     /locus_tag="ACHY09_004442"
     CDS             complement(6222..>6412)
                     /locus_tag="ACHY09_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887319.1"
                     /translation="KNVGGGGIWNITPVPNTDVCVQILRRIEARGGRALGKTGECIEV
                     KTNEPLNNVMETLNGGGQ"
BASE COUNT         1547 a         1784 c         1491 g         1590 t
ORIGIN      
        1 acaaaaagat tattcacctt tcagcggaaa catatactct gtaattatca ggtcagggat
       61 atctgtaatt acactatggt tttttctgtt ctttttctca tcgtccatgc tcagaacgcc
      121 tgttacgggt ctttatctta ccccctttat atccagcctg agaatcgata tacatccaca
      181 atatattacc agaccatctg tcaaagtatc ctcaagaatc aacgcagaca agcttgtcgg
      241 aaatattgaa catttcatat tatgctatcc gtcttataac acgatcacat tacagtctca
      301 catcctgcgc cacggaatcc gaaacagaat agctaataac ctgaaaaaaa aaggtatact
      361 atataaagaa aaggcaagga agacgccact ctgtgaaact attgtcctga atctgaggta
      421 tgggggaaaa acccgctaca gactgttctc ctgcaggagg catcccaatg gattcagagt
      481 acatcgcaat ggcggagcat tcattattca gacgcagaat aattaatctc attttttcag
      541 gagcagatat agcagaataa aactgaacac agaccagcaa agcccgactg gatctttagc
      601 ccgcaaagaa aaagcggcaa tcccggaaaa ataacagccg gatacggtaa tacataataa
      661 cccgggatac cctgcactca acaatccgga taaaaccaga aatacgggga tcaccagtat
      721 acctgtcagc atcacagatg cttttgatga tccccacgga cggtaaaacc agcgttgttt
      781 taatacgttg cctgcagcct caagtaaatc agtctgtctt tccagagaca tcaccacatc
      841 agaatgtcgc ccataaactt caactgcctt catcctggcc atccataact gtgcacacaa
      901 ttcataaatt tgtgcaggtg ttacccgttt tcctccggac aaacgagcag cgacgtgatc
      961 cgtctcttct gcaatctccc tcagagagat aaactgtcgt aatggctccg tgttcagtga
     1021 aagactgacc tgtctgagga ttgatacaag ttgcacttta ctgttgcgca tcacattttc
     1081 ccgattacag gaacacacag ttacgtttac gccacatcag gtaaccgaag tttttctttg
     1141 tgttgggcgg attttttccc gtctcccagc gcatcacact gcgtccgaac ggatggcact
     1201 gtccgctgtc cgggtacata ttcaccatct gataacgcca gcgctctttg ggaagtatgg
     1261 gcgacggata ttcataacag acagccacat ccacgccgat gctgttcatt atcattccct
     1321 gacggtggag cttaaacgcc atccgttcac tgaccagcag ggatgactgc aacggactgg
     1381 attcattgct cacccagcca ttaaacggat acatactgcc ctgcgaaccg gcacaccaga
     1441 acaggatatc cagcggcttg ttaaaggcac tggcaatggc atccaccgca caggctccct
     1501 gtgctatcgg atttgcaaag atgaccgctt ccgggttgag aatggtggtc agactgctgt
     1561 ctgtccaggt agggtcaatt tctgagatgt acccgatatc catatcgccg cccatcagac
     1621 atcctgctga ggtgataata ctcagccagt atgtcagcgg gtatttgtac cagtgaacat
     1681 ggtaaaatgc cccgttcacc tgcttgtcgt cttttttggc cgttccctgc ccggttttcc
     1741 ccagctccgg gattgttatt ccaagattta ccatacatcc cggactgcgg gtcacatccg
     1801 tcagtgccat cggctcccag taaccaatag ccagcccgat acgtttaaat acaggcggtg
     1861 gcgcaggaca gaactggatc ggcattgacg ggtttattgt atccggcacc ttcccctgac
     1921 tcacttttgt gctgcccagc gacagtggaa aaatgcagtt ccagcagata tccgtgatcg
     1981 ggttaacaaa ccgcccttca caggcagcgt ccgctgaggc tgcgggaaca tggccagtca
     2041 gcagcaaaac cacacacaac cacatacacc gtttcatttc cctccctccc cgaccggaat
     2101 aaactccacc ttcaggaaac gatcgccact gacagcactc acccgtgccg gcacctgatc
     2161 aatgccgaga cgctggcaga tcacgccgtt ctgatcaaag taaacacgac tgtccagagc
     2221 cttctgcatc tccgggatac tgccctgcac caggataatt ttgctctcca gtgtgggagg
     2281 cgtctggcgc ttcatccatt caatctgctc aggatcatca ccgttgatga aatacagtgt
     2341 ctggttaaaa ggcacatact gcagcgggtt catcacctcc ccctggcggg cgaagatacg
     2401 accttcgtta tcccggatat ccgcagccag tctgacagac gggtcaaaca ggcggctgct
     2461 gtatttctcc gtccgaccga taccgggcac ggcagggggc cgcaggctgt tgcggatcac
     2521 ccgctcctta aacgcatcca ttttccggcc catctctccg gactgctcca gagctgtcag
     2581 acgctgcata atcaccgtca gcatgtccgc ctcctttacc ggccacaaat ccccccaggt
     2641 gccaagatca gctgcagcca cactctgccc ccataccagc agggcaatca gcccccggca
     2701 tctcatggtt ctgccctcat tcgccgggca atatcctgct ggatttcacg ggtgatatcc
     2761 ggtgccccct gtaccacggc aggcgacacc agaatgacag cgtggtgctt ctgctgccac
     2821 gtctgcagac tggcctccag tgccgcgtta aaacggtcag ccagcgcctt cgactgcgct
     2881 tctgacagtt ttttctggct ggcgctgtca aaaaacgcat ccactgtctg tttcatgttg
     2941 aaggcgacgg ttaccggcgt gttcagtctg acaatgccgt aactgacggc tgcgttcagt
     3001 aacaccattg ccaggcatcc cggcaccgtc caccaccagt ggctgcgtcg acgggctgtc
     3061 acatcagtcg ttttttgcgt tgtggtcatg caacactcct gtatttctca tgttcttcca
     3121 gccatgcttc cagcgaagcc atctccggcc ctgacttctt ccacgccagc tgccacaccg
     3181 cctcatgaat actcagcccc tctttacgtt tctgctgcac aaactcaaaa tccgggccgt
     3241 cagagctgta catggcacgg cttagcgggt ccacaaacag acggtgccag gaggagtggt
     3301 tttccacctg cagcaggaag gaactgaacc actggtcttt ggccgcacca aacttaccaa
     3361 tcatgtcgcg ctgcagtggc aggaactggt ccggatacag ctggttatat ttcgcgaact
     3421 ccttcgcact ctgtttgagg ataattttgt aggaggagtt accccatgcc gcacgggccg
     3481 cactggaggc cttgtcagag tcaaagtcga cgatgttctg tgtgatggtg atataggcac
     3541 cagtatgacg gcgggcggta cggtaacctt tctcaataaa ctcaccgacc ttgtgatttt
     3601 tgaagtccag cagacgccac ccctcatcaa tgacgttcag tttcttgagg ttacgcggcg
     3661 tgcggtacat ccggttctcg atgtaaatga taaggctgaa catcaccgca accagcaggg
     3721 acggacggtc ttccagtcca cccagctcca gcaccaccat ttttgcgtca tcccgcaggg
     3781 acggttcatc agagttaaaa tactggccgt aagtaccgtt ggctgtgtac tggtccagca
     3841 gcactatcat ttcatccaga cggctgcgga tggtcgggga ctcggcatac tggtcgttat
     3901 cccgggcgtt tttcaggaaa tccaccacgt catcaatacg cgcccggttc tgtctggtaa
     3961 gccaggaggc cctgaccgcc tgcagcagca gaccttcatg cacttcatcc aggttaccgt
     4021 tggggctggc catcaccgac agctggtcac ggacacgctc cgctgactgg tcaatatcgg
     4081 tgatgttcgc aaacgggtta aagcgcaggg tttcaccgtc cagatatacg ccccccatgt
     4141 tttcacacag ggacttgtag ccatccccca tgtcgaacac cacagcaaaa cccccggagt
     4201 ccagcacact gcggataagt ggctgtatca gcccggtttt accggccccg gaagtgccac
     4261 agaccgccat gttgtagttg gtgttattca tcccccggaa gaaaatatcg ataaacgcca
     4321 gctggttacg gtaggtgggt gccagcagac ctgccggcgt cagggggtta tccgccacta
     4381 acggcatcag gttggccaca ttaaagctct ctgcgcgctg aaccactccg gcctctttca
     4441 gttgtttaaa caaccctttc ccggccataa agggcaggca ggtcaggaaa ttgcgcatgt
     4501 ggttaaagcg cggtgaaatc agttcaaaac cgtttttacg gaagctgttc aggatatcct
     4561 gctccacttc cagtgccgtt tcattattgt ccttgcagaa tgctgtgatg ttgaggaagt
     4621 aggacacgac agaagactga ccggagccca gccgctgacg cagttccccc cactccttcg
     4681 cttctttctc cacggacgga aaccatttgg cataggaggt cttcgacttt ttctccaggt
     4741 ccatgtactt caggttggct tcgctgtggg ttttcacctg gtcttccacc accagggtca
     4801 gcgtcagaat gaacgggcag gagatggaca gttccgggtt cagcaggttg ctgtagttgt
     4861 cggccatgtt ccacaggaag gcgatttccg ggttacgggc cagatggaaa ttcaggatgc
     4921 gggccgtgct gttcctgccg ttctcacgca gtcccagcgt caggtaatct gcccggacct
     4981 tcaggtcaaa actgtcctcc acgcactgat aattcaaatc agaatacggg tccagctgac
     5041 gtcttttcgg gtacagggag tccgggttat ggttaatcat ctccccgaca atatcgatga
     5101 aagcctgtgc atccaccgtc tgcgtggtga tatatgctcc ctgcagagac gcccggatga
     5161 ttttcaccag gttttccatt tccagaatgt cggcccggct ttttttcttc gagggagaac
     5221 agtacgagat aaacacccgg taatggcgca gggtcagagg caggttcaac ccttccggca
     5281 gcggaaacag tgtttctgcg gctttcatgt aataggcccg ggtaatggcg ttaaaccgtt
     5341 ctgcctgttc acctgaccag gagaactcac gcagcccgta ctcaatccgc tcgccaacca
     5401 gctggctgga catcagatgg atacacagcg gaataccgcg cggcagtttg gtgcgcagca
     5461 tatggtcgag agcctccaca atggtctcat tcgcaccatt gatgggaatg gcttccagca
     5521 taaagcccat tgtggagtca ttcatgaaca gaccggattc ctggttgtaa tcacggtatg
     5581 gcagcagacg gctgaactgc gggaagttca tctcaccgag gatctgatta gcctgagcag
     5641 attcatccgg taatttaagt gcggtcagca gtgaattgac cgcctgagtg acgacctcaa
     5701 gtgggttatt actcatatga gtattcctta gtacgtttaa aaaaggaaaa tatcagtctg
     5761 tggcgtggat atatcacgca gtgccatttc tgaaaccaca gcacaaataa gagatggagg
     5821 cttttaacag gaaaccagga ttacaattaa cgttcctttg ctgaatcagc atcacttaat
     5881 gcctgaagtt gtttttcgcg tatcttattg agctcgtttt ctcttacccg tcgcgcctct
     5941 tccatttcgt ttctttttct gcattctgat gcgattttat ttgccttacc aagtgttaaa
     6001 ggttcagact gataattaag tgtctcctga aatgcttctg ctgcacaggg agtttcctgt
     6061 aaaatctctg tcacttgttc tggtgttgca ggttgttgcg caaggtaaac gatgtaaaca
     6121 ggtatacata caatcatagc cataacgcca acaaatataa aagccagaac agattttaaa
     6181 tatatcgact tcatttcatt ttccttcgaa gcagtaagca ctcattgccc accaccattc
     6241 agtgtttcca tcacgttgtt caaaggttca tttgttttaa cttcaataca ctcccctgtt
     6301 tttcctaagg ccctgcctcc acgcgcttct attctccgaa ggatctgtac acatacatcc
     6361 gtatttggca caggtgtaat attccagata ccaccaccgc caacattttt ca
//
LOCUS       DAZUCC010000242         6365 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZUCC010000242 DAZUCC010000000
VERSION     DAZUCC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.243"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1218
                     /locus_tag="ACHY09_004443"
     CDS             <1..1218
                     /locus_tag="ACHY09_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3887320.1"
                     /translation="ELMTARDDLEQQYARECELTGELLESRSDISRQSETLHLQQALH
                     DIQQNQPLLSVDVDVRTVAGVVADRTGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVA
                     LESIAQRLRAANTGLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINL
                     SEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQ
                     MFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTESELHELIRPLLRD
                     HFQPALLARFQTVIYRPLTQSAMRIIVEMKLAQVCERLHCHYGLSTSVDERVYDALTS
                     ACLLPDTGARNVESLLNQQLLPVLSRQLLSYLAARQHPTYLSLTWNDEDGIVIAFDGI
                     NSDYPQESIYGQC"
     gene            1205..1846
                     /locus_tag="ACHY09_004444"
     CDS             1205..1846
                     /locus_tag="ACHY09_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887321.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTRHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            1843..2070
                     /locus_tag="ACHY09_004445"
     CDS             1843..2070
                     /locus_tag="ACHY09_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887322.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            2149..4668
                     /locus_tag="ACHY09_004446"
     CDS             2149..4668
                     /locus_tag="ACHY09_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFS3887323.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPMLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            4665..6347
                     /gene="tle1"
                     /locus_tag="ACHY09_004447"
     CDS             4665..6347
                     /gene="tle1"
                     /locus_tag="ACHY09_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFS3887324.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVV
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PEFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSE
                     LIFLADKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
BASE COUNT         1678 a         1314 c         1743 g         1630 t
ORIGIN      
        1 gagctgatga cagcacgtga tgacctggag cagcagtacg cccgggagtg cgaactcacc
       61 ggtgagttac ttgaaagtcg cagcgatatt tcccgtcaga gtgagacact gcatctgcag
      121 caggcactgc acgacattca gcagaatcag cccctgctca gtgtggatgt tgacgtacgc
      181 accgttgccg gtgtggtcgc ggaccggacc ggggtgccgt tatcttccct gatgaaggac
      241 gaacagacgg aactgctgca tctggaaaag gatatcggca gacgggtggt cggacaggac
      301 gtggcactgg aatccattgc gcagcgtctg cgtgcggcga atacgggtct gacatccggt
      361 aacggccccc agggcgtgtt cctgctggtc ggtcccagtg gtgtgggaaa aaccgaaacg
      421 gcactggcgc tggcagatgt gatgtacggc ggcgaaaaat cactgattac catcaatctg
      481 tcggaatacc aggagcccca tacggtttcc cagcttaagg ggtcaccgcc cgggtacgtg
      541 ggatacggtc agggcggtat tctgacggag gctgtgcgta aacgccctta cagcgtggtg
      601 ctgctggatg aagtggaaaa ggcccaccgg gatgtgctga atctgttcta ccagatgttt
      661 gaccggggct ttatgcgcga cggtgagggg cgtgaaattg acttccgtaa tacggtcatt
      721 ctgatgacct ccaatctggg cagtgacctt ctgatgcagc aactgagcga aaagccggag
      781 acaacggaat cggagctgca tgagcttatc cgtccactgc tgcgcgacca cttccagcca
      841 gcactgcttg cccgtttcca gaccgtgatt taccgtccgc tgacgcagtc tgccatgcgc
      901 atcattgtgg agatgaagct tgcacaggtc tgtgaacgcc tgcactgcca ttatggactg
      961 agcacatcgg ttgatgaacg tgtgtacgat gccctgacat ctgcctgcct gctgccggat
     1021 acgggtgccc ggaatgtgga gagtctgctg aaccagcaac tgctgccggt actgagccgg
     1081 cagttgctca gctatcttgc tgccagacag catccgacgt acctgtctct gacgtggaat
     1141 gatgaggatg gtattgtgat agcgtttgat ggaatcaata gcgattatcc gcaggaaagc
     1201 atatatggac agtgctaaag aaattgcccg ggatatgctg gaggccgtaa atattaatgg
     1261 ggatgggttt ctgggggccg tcgggaaggg actcatatct ttgccagtca gtctcggtta
     1321 tatgggatat gattttatag ataccaggca taggcgggaa aaccagaatg acaaattcag
     1381 aatggccaga ctggtagaaa aaggcatttt cagccgtgat attattggga aaattatcag
     1441 tatttgtctt gaagactttg tatcccggat caatatggaa aaaacaggct ccattgtgaa
     1501 gaacgtttca ggagatctgg caggtagaat ggcatttgct gaattgaccg gggttaaact
     1561 gggagaggtt ctggtatcca gagggctggc tgcttttttt gcagggagtt ttgccgggtt
     1621 gttattatct gttggcgcag agacatcgcg tgcgatttac acatcccgtt ttctgaaaga
     1681 cagaaatccg gtgttgcatg ataagctgaa agctcagggg gatttggatc ttttgtactt
     1741 tcttgttgaa gatattgtgc gcccgtttga acgagcctgt attgtcgcag accgaaattc
     1801 ggctgagttt gaccggatat gcgaatattt tttcggtggt ttatgaagat aaaaagaaca
     1861 ttacttataa tgctttcccg atttatccgt ggcgcaggta tggggttagg tgcgtcgggt
     1921 attgtattta cagtctggtt cttttttctc tcatctgctg aatttaaata tctgtgggga
     1981 gggctctctg ttgcgttatt ttttgtcggt tatctgatgt atcgatttgc atatgcatat
     2041 atttacgatg aatgggatga ttatcagtaa ttctctctga aaataaagct taacggtcat
     2101 cattatataa ttgcagttca ataccggtac agacatggag ttaacaatat gagtctgaca
     2161 gatacacttt caaaaactat ctctgcttcc ctgaatcgtt accatttaat tattccctcc
     2221 tgcccgtcac cactggatgt tgagcatttc agcggcaggg aggagatgag tcagacctat
     2281 tattacatca tcaattttac cagtactgac aacgacctgg atgccgggct gctgctgcgt
     2341 aaatcagcca catttacaat gggtacggga gggcttaagg agcaggtgag ccagaaagta
     2401 gtacatggtg tcattacaga ttttcgccgt atatcgggct ccgccgacca ggcaaaatac
     2461 caggtgatac tggaaccatt tattaaacta ctggacaggc agcatcgttc tcaccgattt
     2521 ttcgttaaca aatcggtgcc ggaggtggtg acagaggttc tccaggagca cggcctgaaa
     2581 ggctgggagt ttgagttccg tctcaaaaaa acgtatccga agcgtgaaca gattaaccag
     2641 tatcaggaaa gtgacctgca atttattcag cgtttgctgg ctgaagtggg gattttttat
     2701 tttttcaccc tgcaaccgga cacgcagacg gaagtagtgc attttggtga cagccaggct
     2761 gcgctgacgt ttgataagac gctggcgata aacagtccgt ccggaatgaa tgataacaga
     2821 gcggattcag tgtgggggct gaatgtgacg cacaatgtgg cagaggccag tgtcaccaca
     2881 aaggcttata accatcgtga agcacaatat ctgttgcagt ccgccccggc agacatgacg
     2941 catggtgatg gtgacggaat taactatggt gaggtgtacc actaccattt ccgtcatctg
     3001 gaacgggggg ataaaatcga cccggtgccg gagacggcca atttttatgc ccgccttggt
     3061 catgaacgtt atctggcgga gcaggtgcgg ataaccggca acagtacaga cgccacgctg
     3121 gcacctgctc aggttctgac tatcaccgac agtcttccgc cgatgttgcc tgcgctgctg
     3181 cgcaatccgg tgttgcttac ctgtgtcggt ttttctgcaa gtcgcaagga tgccctgcag
     3241 gttgtactga agggcgttcc ttacagtgaa gttatctgct ggcgccctcc tttactgccg
     3301 cgtccgaaag ttaccggtac catgacggcc cgtgtgacca gtgcaaaaga aggggatatc
     3361 tacgcctggc aggatgcatc aggtatgtac cgggtgaaat ttgatgctga ccgtgatgat
     3421 aaaaatccgg gacaggaaag catgccggta cgtctggcca aaccctacag tggtgatgct
     3481 tatggttttc attttccgct gattcaggga acggaagtgg ccattgcctt tgaggaaggt
     3541 gatccggatc gcccctatat cgcgcatgcc ctgcatgatt cacgtcatgt tgatcacgtt
     3601 accgataaga acggtacgcg caatgttatc cgtacgccgg caaataacaa gttgcggatg
     3661 gaggacaaac gcggtgagga gcatatcaag atcagtaccg agtttggcgg taagacacaa
     3721 cttaatcttg gtcataacgt taatgcctcc cggaaactca ggggagaagg cgctgagctg
     3781 aggactgatg actggataag tatccgggga ggaaaaggaa tatttatcag tgcagatatg
     3841 cagcctcagg cacaaggaaa gatgctggat atgaatgaag ctatccgcca actggagcag
     3901 gcattatctc tggcccgaag tatggcgaag gccgcaactg ctgcaaatgc tactcaggga
     3961 gatataagtt gtcagcaacg gttgaattca tcgctaacag atctcactgc gccgggaatg
     4021 ctgttgcatg ctcctgatgg gattggcatg gttagtgcac gggcgttacg aattgcttcg
     4081 ggtagtgaaa gcgtgggcat aatgtcggga gataacactg atattagtgc cgggcagtca
     4141 tttacagttg tagcagaagg tgctgttagc ctgttgagca gaaaccaggg gatgcaactg
     4201 ttagcagcaa aagggcgggt aaatattcag gctcaaagtg acgatttgtc aatgagttcc
     4261 caacagaatc ttgatattca aagttctgag ggcaaagtga ctgtttctgc taatcaggaa
     4321 ctgatactgg catgtggtgg agcttatatc aaactaagcg gtggaaatat tgagctggga
     4381 tgcccgggac agatcctgtt aaaaagcacg aatatgcaga agatgggacc aacaagtctt
     4441 gatattgcgt ctgttgaaat gccgcgaggg tttggtggcg gttttattct taccgatgaa
     4501 gcgggagtgc cgcaaccttc gacgccttat cgattaacaa cggctgaagg tgatattcta
     4561 caagggatca ccgatgaaaa tggtaaaaca gcgccagtca acacctccat tccgtcagta
     4621 gtaaaagttg agtttgggaa ggtaaaaatt catggagaaa cagaatgaca aaatatcagg
     4681 gatatgacgt aacagatgcc acccataaaa ccagtattca taatgactgg aaagttgttg
     4741 ttgcaaagaa aaaaccggcg cggggtgtga ctttaaccat tggtatattt ttcgatggta
     4801 ccggaaacaa tcgggagaat acagcatctc gcctgatgaa atttaatgag tgtagtgcag
     4861 ccagacaggg ggtaaatcaa aaagatgcac aaagctgtga ggactttctt gaagaaatta
     4921 acaagaacag tatctcgaat ggcagttatc gaggatatta ttccaatatt cactggctaa
     4981 attcacttta cgtagcagat cagactttgt tggaaagtca ggttgaagca caaattcaga
     5041 cttacatttc cgggataggg actgcggctg gtgaagcaga ttcagtcatt ggcatgggat
     5101 taggcaccag tattctggat atatttgaag gtgtggtgac gaaaaccgac gaggcaatgg
     5161 aacgaatcac ccaggcgtta tcagaattta tggggttcaa tttaaatcct gatttttgta
     5221 tcgcaaaaat ccagtttgat gtttttggtt ttagccgggg agcagcggcg gcaaggcact
     5281 ttgccaatcg ggttatggaa caggatcctg ctatagccag agctattgct aaagggctca
     5341 gaggcgattt ttatgatggt aaaccttctg gtgaagtccg ttttctgggg ctttttgata
     5401 ctgtcgcagc tattggtgga atcagtaatt tcttcgatat taatggacgt agcaacccgg
     5461 gagtaaagct ggaattgcgt ccttcagtcg tgaagaaagt tttccagatt actgcaatga
     5521 atgaataccg atataatttc agcctgaaca gtattaaagg tatgtggccc gaactggctt
     5581 taccaggggc gcattcagat attggaggtg gttacaatcc ggtaggttca cctctgcagg
     5641 aaaatgaaag tctgtttctg agttgcccgg aatttgaaat tgtcagtgac gataccagag
     5701 aaacggatac gcgagtatac cgtaatgcag agcaagccag aaaaatgttg atgactctgc
     5761 cagccttaaa acatattttg cctcatggaa aactaaccac caaaattcgt tccgtaggtg
     5821 tgaacaacag caatcaacgt cgggctggtg tgatacaaaa gcaggtggga gccgcagtat
     5881 tttttgagcg tatggcagta cctaatgact gggctaatgt ttgcctgcgt gtgatgctgg
     5941 atgctgctca ggaagcaggg gtattgtttg aaccaattcg acaaacaaat actgagttac
     6001 aacttccatc tgaactaata tttcttgctg acaaagccat tgctcaggga aaagcggtac
     6061 gtttggggca agagcctcag gcttttactg aggaagagtt gtatattatc ggaaaataca
     6121 cccattgttc tgccaactgg aatattgagt ctgatggtaa tttatgggtg gatcctacaa
     6181 cgggagagat ttttatccat cgctttggtc ctaaaggtaa taaggcgttc gtttttccga
     6241 ataaaccaaa tgatcgctgg attcgttctg tatggtacat ggatgatcag cagcgattga
     6301 atgataacgc agtaaaaaat actaaagtta tgatgtctgg tgtttaacac cacagggtgg
     6361 caata
//
LOCUS       DAZUCC010000243         6191 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZUCC010000243 DAZUCC010000000
VERSION     DAZUCC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.244"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            148..1368
                     /gene="astC"
                     /locus_tag="ACHY09_004448"
     CDS             148..1368
                     /gene="astC"
                     /locus_tag="ACHY09_004448"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFS3887325.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFAYRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRCGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSGGSS"
     gene            1365..2399
                     /gene="astA"
                     /locus_tag="ACHY09_004449"
     CDS             1365..2399
                     /gene="astA"
                     /locus_tag="ACHY09_004449"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFS3887326.1"
                     /translation="MMVIRPVERSDVAALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEESETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLAANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            2396..3874
                     /gene="astD"
                     /locus_tag="ACHY09_004450"
     CDS             2396..3874
                     /gene="astD"
                     /locus_tag="ACHY09_004450"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFS3887327.1"
                     /translation="MTLWINGDWVTGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVMAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGRWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            3871..5214
                     /gene="astB"
                     /locus_tag="ACHY09_004451"
     CDS             3871..5214
                     /gene="astB"
                     /locus_tag="ACHY09_004451"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFS3887328.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAS
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            5207..6175
                     /gene="astE"
                     /locus_tag="ACHY09_004452"
     CDS             5207..6175
                     /gene="astE"
                     /locus_tag="ACHY09_004452"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFS3887329.1"
                     /translation="MDNFLALTLTGKKTVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         1318 a         1586 c         1870 g         1417 t
ORIGIN      
        1 tggttataac tccttgattt ccagttagcc tccgccgttt atgcactttt atcactggct
       61 ggcacgaacc ctgcaatcta catttacagc gcaaacatta cttattatta acatataaat
      121 aacgaattat ttactgtaga ggtcgctatg tctcagccaa ttacgcgtga aaactttgat
      181 gaatggatga tacctgttta cgctccggca ccgtttatac cggtacgtgg cgaaggttcg
      241 cgcttgtggg atcagcaggg gaaagagtat atcgacttcg cgggtggcat tgcggtgaac
      301 gcgctgggcc atgcgcatcc ggaactgcgt gaggcgctga acgaacaggc gagtaagttc
      361 tggcataccg gcaacggtta caccaacgag ccggtactgc gactggcgaa aaaattgatc
      421 gacgccacgt ttgcctatcg cgtcttcttt tgtaactccg gtgcggaagc caacgaagcg
      481 gcgctaaaac tggcgcgtaa attcgctcac gaccgctacg gcagccataa gagcggtatc
      541 gtggcgttta aaaatgcgtt tcatggtcgc acgctgttta ccgtcagtgc gggcggtcag
      601 ccagcctatt cacaggattt tgcgccactg ccgccggata ttcgtcatgc cgcatataac
      661 gatattaact ctgccagcgc gctgattgac gactctacct gtgcggtgat tgtcgaaccc
      721 atccaggggg aaggcggtgt ggtgccagca agcaacgcat ttttacaagg tctgcgtgaa
      781 ttgtgtgacc gccacaatgc gctgttgatt tttgatgaag tacaaaccgg cgtcgggcgc
      841 accggggaac tgtatgccta tatgcactac ggcgtgacgc ccgatctgtt aactaccgcc
      901 aaagcactgg gcggcggttt ccccgttggc gctctgttgg caaccgaaga atgcgccagc
      961 gtgatgaccg tgggcacaca tggtaccact tatggcggta atccgctggc ctcggcggtg
     1021 gcaggtaaag tgctggatct catcaacacg ccagagatgc ttaatggcgt taaacagcgt
     1081 cacgactggt ttgttgagcg tcttaatacc attaatcacc gctgcggttt gttcagtgaa
     1141 attcgcggat taggtctgct gattggctgt gtgctgaatg ccgattacgc cgggcaagcg
     1201 aaacaaattt ctcaggaagc ggcgaaagcg ggcgtaatgg tgctgattgc cggtggcaac
     1261 gtagtgcgtt ttgcgccagc gctcaatgtc agcgaagaag aggtgacgac cgggctggat
     1321 cgttttgcgg cggcttgtga acactttgtg agcggaggtt catcatgatg gtcatccgtc
     1381 ccgttgagcg ctcagacgtt gcggcgctga tgcagcttgc cagcaaaacg ggcggcggcc
     1441 tgacgtcgct ccctgctaat gaagccacgc tttcagcgcg tatcgaaagg gccatcaaaa
     1501 cctggcaggg cgaactgccc aaaagcgagc agggttatgt gtttgtactg gaagagagcg
     1561 aaacaggcac tgtggcgggg atttgcgcca ttgaggtggc ggttgggctg aacgatccct
     1621 ggtacaacta tcgcgtcggc acgctggttc acgcctcaaa agagctgaat gtctataacg
     1681 cgttgccgac gctgtttctc agtaacgatc acaccggcag cagcgagctg tgcacgctgt
     1741 ttctcgaccc ggactggcgc aaagagggca acggctattt gctgtcgaaa tcgcgcttta
     1801 tgtttatggc ggcctttcgc gacaagttta atgacaaagt ggtcgccgaa atgcgcgggg
     1861 tgattgacga acatggctat tcgccgttct ggcaaagcct cggtaaacgc ttcttttcga
     1921 tggattttag ccgcgccgat tttctctgtg gcaccgggca aaaggcattt attgcggaac
     1981 tgatgccgaa acatccgatc tatacccact ttttatccca ggaagcgcag gacgttatcg
     2041 gccaggtaca tccgcaaacc gcgcccgccc gcgcggtgct ggagaaagaa ggttttcgct
     2101 accgcaacta tatcgacatc tttgacggcg ggccgacgct tgagtgtgac atcgaccgcg
     2161 tgcgcgccat ccgcaaaagt cggctggtgg aagtggcaga agggcagcct gcgcagggcg
     2221 atttcccggc ctgcctggcc gccaatgaaa attatcacca tttccgcgtg gtgctggtgc
     2281 gtaccgatcc ggcaaccgag cgtttgatat taaccgccgc acaactggat gccctgaaat
     2341 gccacgccgg ggatcgcgtt cgtctggtgc gcctgtgcgc agaggagaaa acagcatgac
     2401 tttatggatc aatggtgact gggttacggg ccagggcgca tcgcgtgtga agcgtaatcc
     2461 ggtatcgggc gaggtgttat ggcaaggcaa tgatgccgat gccgctcagg tcgggcaggc
     2521 ttgtcgggct gcccgtgcgg cgtttccgcg ctgggcgcgt ctctcctttg gcgatcgtca
     2581 ggtaagagtt gaacgctttg ccggattgct ggaaagcaat aaagccgaat taaccgcgat
     2641 tattgccaga gaaacgggta agccgcgctg ggaagcggca accgaagtga tggcgatgat
     2701 caataaaatc gcgatatcaa ttaaggcgta tcacgttcgt accggcgagc agcgtagtga
     2761 aatgccggac ggcgcggcga gcctgcgaca tcgcccgcac ggcgtgctgg cggtgtttgg
     2821 gccgtataat ttccccggtc atttaccgaa cggacatatc gttccggcat tgctggcagg
     2881 taacaccatt atctttaaac ccagcgaact gacaccgtgg agtggcgaag cggtaatgcg
     2941 tttatggcag caggctggct tgccgccggg cgtgctgaac ctggtgcagg gcgggcgtga
     3001 aacgggtcag gcgctgagtg cgctggagga tctcgacggc ttgctgttta ccggcagcgc
     3061 caataccggc taccagctgc atcgccagct ttccggtcag ccggagaaaa ttctcgccct
     3121 tgagatgggc ggtaataacc cgctaattat cgatgaggtg gcggatatcg atgccgctgt
     3181 ccatctgacc attcagtcgg cgtttgtcac tgccgggcaa cgctgcacct gcgcccgccg
     3241 tttattgctg aaaagtggaa cgcagggcga tgcatttctt gctcgcctgg ttgccgtcag
     3301 ccagcggtta acgccgggcc gttgggatga cgaaccgcag ccgtttattg gcgggctgat
     3361 ttctgagcag gccgcacagc aggtggttac tgcctggcag gaactggagg cgatgggcgg
     3421 acggaccctg cttgcgccgc gcttattaca agcagggaca tcgttgctga cgccggggat
     3481 cattgaaatg acaggcgtta ctggcgtgcc ggatgaagaa gtgtttgggc cgttattgcg
     3541 cgtctggcgt tatgacaatt tcgatgaagc gattcgaatg gcgaataaca ctcgcttcgg
     3601 cctctcttgc ggtctggttt cccccgagcg ggaaaaattc gatcaactgt tgctggaggc
     3661 acgggcgggg attgttaact ggaacaaacc gcttaccggt gctgccagta ccgcgccatt
     3721 cggcggcatt ggcgcttccg gtaaccatcg ccccagcgcc tggtatgccg cagattattg
     3781 tgcctggccg atggcaagtc tggagtcgga ctcgttaaca ttgcccgcaa cgcttaaccc
     3841 cgggctggat ttttccgatg aggtggtgcg atgaacgcct gggaaatcaa tttcgacggg
     3901 ctggtagggc tgacgcatca ttacgcgggc ctgtcgtttg gtaatgaagc ctccacccgt
     3961 caccgttttc aggtctctaa cccgcgactg gcggcgaagc agggcttact gaaaatgaaa
     4021 gcccttgccg acgcgggatt cccccaggcg gtgatcccgc cgcacgagcg tccgtttatt
     4081 ccggtgctgc gtcagttggg attcagcggc agcgatgagc aggtactgga aaaggttgcc
     4141 cgccaggcac cgcactggct ttccagcgtc agctccgctt cgccaatgtg ggtagccaat
     4201 gcggcaacga tcgcgccatc tgccgatacg ctggatggca aagtgcatct caccgttgcc
     4261 aacctgaaca ataaatttca ccgttcgctg gaagcccccg tcaccgaatc gttgttaaaa
     4321 gcgattttta acgacgaaga gaaatttagt gtccattcgg cgttgccgca ggtagcgttg
     4381 ctcggtgatg agggggcggc aaaccacaat cgtctcggcg gtcattacgg tgaaccgggt
     4441 atgcaacttt ttgtctacgg gcgagaagaa ggcaatgata cccggccttc ccgttatccg
     4501 gcgcggcaga ctcgcgaagc cagcgaggcg gtggcaaggc tgaatcaggt gaatccccaa
     4561 caggtgattt tcgcccagca aaacccggac gttatcgacc agggcgtttt tcataatgac
     4621 gtgattgccg tgagtaaccg ccaggtgctg ttttgccatc aacaggcgtt cgctcgccag
     4681 tcacagttac tggcaagcct gcgtgcgcgg gtgaacggtt ttatggcgat agaagttccg
     4741 gcgactcagg tttccgtgtc agatgcggtg tctacatatc tgtttaacag ccaactgctg
     4801 agccgcgatg atggttccat gatgttggtg ctgcctcagg agtgtcggga acacgccgga
     4861 gtatggggtt atctcaatga actccttgcc gctgacaacc cgattagcga actaaaagtc
     4921 tttgatttac gtgaaagcat ggcgaatggc ggaggcccgg cgtgcctgcg gttgcgcgtg
     4981 gtattgacag aagaagaacg ccgggcagtg aatccggcgg tgatgatgaa cgatacgctg
     5041 tttaatgcgc tcaatgactg ggtggatcgt tactaccgcg atcgccttac tgctgccgat
     5101 ctggccgacc cgcaattgct gcgcgaaggg cgggaagcac tggatgtatt gagccaatta
     5161 ctgaatctcg gttcggttta tccgttccag cgcgagggag ggggaaatgg ataattttct
     5221 tgctctgacc ttaacgggta aaaaaacggt tatcaccgag cgagaaatca acggcgttcg
     5281 ctggcgctgg ctgggcgatg gcgtgctgga actgacgcca ttaacgccac cgcaaggcgc
     5341 gctggtgatt tcagcgggga tccacggtaa tgagacggca cctgtggaaa tgctggacgc
     5401 gttgcttggc gcgatatctc acggtgagat cccgttacgt tggcggttac tggtgatcct
     5461 cgggaatcct cctgcgctga agcaagggaa acgttattgc catagcgata tgaatcgaat
     5521 gtttggcggt cgttggcagc tatttgctga aagcggggaa acctgtcggg cgcgcgaact
     5581 ggagcagtgc ctggaagatt tttatgacca gggcaaagaa tctgtgcgct ggcaccttga
     5641 tctccatacc gcaattcgtg gctccttgca tccgcggttc ggtgtattac cgcaacgcga
     5701 cattccctgg gacgagaaat ttctgacgtg gctgggtgcg gcgggtctgg aggcactggt
     5761 gttccatcag gagcctggtg gtacgtttac ccatttcagc gccagacatt ttggcgcgct
     5821 ggcctgtacg ctggaactcg gcaaagcgtt gccctttgga caaaacgatc ttcgccagtt
     5881 tgcagtcact gccagcgcaa ttgctgcgct gctatctggt gagagtgtcg gtatcgtgag
     5941 aacgccgccg ctccgttatc gggtggtttc gcaaattact cgccactcgc cgtccttcga
     6001 aatgcatatg gcaagtgaca cgctgaattt tatgccgttt gagaaaggaa cattgctggc
     6061 gcaggacggg gaggaacgtt ttaccgtaac ccatgatgta gagtatgtgt tattccctaa
     6121 tcctttggta gcgttgggat tacgcgcggg attaatgctc gaaaaaataa gctaatactt
     6181 acccgcagaa a
//
LOCUS       DAZUCC010000244         6178 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZUCC010000244 DAZUCC010000000
VERSION     DAZUCC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.245"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..189
                     /locus_tag="ACHY09_004453"
     CDS             <1..189
                     /locus_tag="ACHY09_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887330.1"
                     /translation="NNLTINYGTETSPELSGISYEDANALITELILFIEDVDAENHEA
                     YQGHLNEITRILNFAIAA"
     gene            complement(241..831)
                     /gene="cobO"
                     /locus_tag="ACHY09_004454"
     CDS             complement(241..831)
                     /gene="cobO"
                     /locus_tag="ACHY09_004454"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFS3887331.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(828..1586)
                     /locus_tag="ACHY09_004455"
     CDS             complement(828..1586)
                     /locus_tag="ACHY09_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFS3887332.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            1806..2855
                     /gene="sohB"
                     /locus_tag="ACHY09_004456"
     CDS             1806..2855
                     /gene="sohB"
                     /locus_tag="ACHY09_004456"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFS3887333.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(2891..3142)
                     /locus_tag="ACHY09_004457"
     CDS             complement(2891..3142)
                     /locus_tag="ACHY09_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFS3887334.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3522..6119
                     /gene="topA"
                     /locus_tag="ACHY09_004458"
     CDS             3522..6119
                     /gene="topA"
                     /locus_tag="ACHY09_004458"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS3887335.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1594 a         1470 c         1641 g         1473 t
ORIGIN      
        1 aacaatctga cgattaacta cggtactgaa acttcgccag agttaagcgg aatcagttat
       61 gaagatgcca atgcgttaat tactgaactg atattattta ttgaagatgt tgacgctgag
      121 aatcatgaag catatcaggg acatttgaat gagattacac gtattctgaa tttcgctatt
      181 gccgcttgaa tataatcatg attaaagcac ctcaagaggc ttcttttgag gtgctttcac
      241 ttagtagtca atccctatct gcgcttttac accggcatca aacgcatgtt tcacggggcg
      301 taattcactc accgtatccg ccagttcaag aatatcccga tgacagcctc gacccgtgat
      361 aatcaccgtc tgttgatgtg gacgttcatt taacgcctgc accacttctt ccagtggcaa
      421 atagtcatac gccaccatat acgtcagttc atcgagcaaa accatatcca gtgaaggatc
      481 ggccagcatt cgtttggcat gttgccagac ttcacggcag gccgcggtat cagattcgcg
      541 attttgtgta tcccaggtaa agcccgttgc catcacctga aactcaacgc catgtggctc
      601 cagtagattg cgctcgccat taggccaggt gcctttaata aactgcacga tacccacttt
      661 tttgccatgg cctactgcac gagttgccgt accaaatgcc gcggtggttt tgccttttcc
      721 attgccggta aagacgatga taataccgcg ctcatcctgg gcctgggcca cacgagcatc
      781 gactttttct ttcactcgct gctgacgctg ttggtagcgt tcatcactca ttgggaaatt
      841 cctggtttac ggcccggttg ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg
      901 cccatcagcc agaggtagag cggcatgata tcagcgggtg ttttaagttt ctgtggatct
      961 tcggtcggga aggcgctggc acgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg
     1021 acgcgcaggc gctgctgata ttcatcggcc agtacctgca tcatcccttc ggtggcaaat
     1081 ttcgacgctg catatgcgcc ccagttggct cgtccctgac gtccaacgct tgatgaagta
     1141 aagaccagtg aaccggcgtc cgatttgagt aataaaggaa gcagtgcctg ggtgagcata
     1201 aaggtggcat taacgttgac ctgcatgacg tcctgccaga cctgcggatt ttgttcgctc
     1261 attgggcaaa catcgccgag caatccggca ttatgcaaaa caccatccag acgcggataa
     1321 ttaacggcaa tgcgctgtgc cagttgttgg caatcttcgg acgtgcaggt cagcaaatcg
     1381 agaataaacc actgtggctg acgtccagtt tcttcgttta tgtggctggc tacctgacgt
     1441 aatttttctt cattacggcc caacagaatc actgtcgcac catagcgtgc atacgtcatc
     1501 gcggcttcac ggccaatacc atcgctggct cccgtcacca ggataatgcg atcattgagt
     1561 aaatcttgtt ttggctggta atgcatggct actcctcaac gacgttgtct gtcgcgctgc
     1621 cagtgtactt tatgactttg gggctttatg cctgaaacag aagggcattt caatcagcct
     1681 ggggcgataa caccgcaaaa tggatacttt gtcatacttt cgctgcaata acatctctgc
     1741 gagacggctt aacatgcctg ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg
     1801 gactcgtgga attgttgtct gaatatggtt tgtttttggc gaaaatcgtt accgttgtgc
     1861 tagcgattgc ggcgattgcc gccattattg tcaatgttgc tcaacgtaat aaacgccagc
     1921 gtggcgagtt acgggtcaac aatctcagcg aacagtataa ggagatgaaa gaagaactgg
     1981 ccgcggcgct gatggactca catcagcaaa aacagtggca caaagcgcag aagaaaaagc
     2041 acaagcaaga agcgaaagca gcaaaagcga aagccaaact gggcgaggtg gcaactgaca
     2101 gtaaacctcg cgtctgggtg ctggatttta aaggcagcat ggacgcccat gaagtgaact
     2161 cgctacgtga agagataacg gcggtactcg cagcattcaa accgcaggat caggttgtgc
     2221 tccgtctgga aagccctggt ggcatggtgc atggttacgg gttggcggct tcgcagctgc
     2281 agcgtctgcg tgataaaaac attcctttaa ctgtaacggt agacaaagtc gctgccagcg
     2341 gcggttacat gatggcctgt gtggcggaca aaattgtttc cgcaccgttt gctattgtgg
     2401 gttccattgg cgtggtggcg caaatgccca actttaaccg cttcctgaaa agcaaagata
     2461 ttgatatcga actgcacacc gccgggcagt ataagcgcac gctgaccttg ctgggtgaaa
     2521 acaccgaaga agggcgggag aaattccgcg aagagctgaa cgaaacgcat cagttattta
     2581 aagattttgt gaagcgtatg cgtccgtctc tggatattga acaggtggca acgggtgaac
     2641 actggtacgg acaacaggcg gtagagaaag gcctggttga tgaaatcaac accagtgatg
     2701 aagttattct tagcctgatg gaaggccgtg aagtggtaaa tgtacgctat atgcagcgta
     2761 aacgactcat tgaccgattc accggcagcg cggcagagag cgccgatcga ttgttgctac
     2821 gctggtggca gcggggtcaa aagccattga tgtaaaagac aaacgcgagg gtaagacctc
     2881 gcgttttgct ttaatcaacc aggtgatatt tttctgaaag cacatgggcc aggtgtttga
     2941 acatattaaa caccgcggtg cttttggctg ttggcaatcc ttgttcatct aaaaagtagt
     3001 cgccggtaaa taccagcacg ccattacgct gcgtgacgcc ggtggcttca atccctgcga
     3061 gcgtatcctc atgctcacga atgattttgt tggcctcttt caacagcgtc tcgcggtcga
     3121 tgggttgtgt ctctttgttc attatttact ccttaaacaa ggacattagt ctacgccagg
     3181 catggcttgc agacaaatat accacgctgg tggcaagagc gccttactgg caactttgga
     3241 ttttgcatgc taataaagtt gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc
     3301 aatctggcaa tagatttgct tgacattcga ccaaaattcc gtcgtgctat agcgcctgta
     3361 ggccaagacc tgttaactca gtcacctgaa ttttcgtgaa cagagtcacg acaaggggtt
     3421 gatatccgca gagagcgagt ccatatcggt aactcgttgc cagtggaagg tttatcaacg
     3481 tgcgacgcat tcctggaaga atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca
     3541 tcgttgagtc cccggcaaaa gccaaaacga tcaacaagta tctgggtagt gactacgtgg
     3601 tgaaatccag cgtcggtcac atccgcgatt tgccgaccag tggctcagct gccaaaaaga
     3661 gtgccgactc tacctccacc aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc
     3721 tcgtcaaccg tatgggggtt gacccgtggc acaattggga ggcgcactat gaagtgttgc
     3781 ctggtaaaga gaaggtcgtc tctgaactga aacaactggc tgaaaaagcc gaccacatct
     3841 atctcgcaac cgaccttgac cgcgaagggg aagccattgc atggcacctg cgggaagtga
     3901 ttggtggtga tgatgcgcgc tatagccgag tggtgtttaa cgaaattact aaaaacgcga
     3961 tccgccaggc atttaacaaa ccgggtgagc tgaatattga tcgtgttaat gcccagcagg
     4021 cgcgtcgctt tatggaccgc gtggtggggt atatggtttc gccgcttcta tggaaaaaga
     4081 tcgctcgtgg tctgtctgcc ggtcgtgtgc agtcggtggc ggttcgcctg gtggtcgagc
     4141 gtgagcgtga aattaaagcg ttcgtaccgg aagagttctg ggaagtcgat gccagcacga
     4201 ccacgccatc tggtgaagcg ttggcgttgc aggtgactca tcagaacgac aaaccgttcc
     4261 gtccggtcaa caaagaacaa actcaggctg cggtaagtct gctggaaaaa gcgcgctaca
     4321 gcgtgctgga acgtgaagac aaaccgacaa ccagtaaacc tggcgctcct tttattacct
     4381 ctacgctgca acaagctgcc agcacccgtc ttggatttgg cgtgaaaaaa accatgatga
     4441 tggcgcagcg tttgtatgaa gcaggctata tcacttacat gcgtaccgac tccactaacc
     4501 tgagtcagga cgcggtaaat atggttcgcg gttatatcag cgataatttt ggtaagaaat
     4561 atctgccgga aagtccgaat cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag
     4621 cgattcgtcc ttctgacgtc aatgtgatgg cggaatcgtt gaaggatatg gaagcagatg
     4681 cgcaaaaatt gtaccagtta atctggcgtc agttcgttgc ctgccagatg accccagcga
     4741 aatatgactc cacgacgctg accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc
     4801 gtattttgcg ctttgatggc tggacaaaag tgatgcctgc actgcgtaaa ggcgatgaag
     4861 atcgtatctt accggcagtc gataaaggcg atgctctgac gctcgttgaa ctgacaccag
     4921 cccagcactt taccaaaccg ccagcccgtt tcagtgaagc atcgctggtt aaagaactgg
     4981 aaaaacgcgg tatcggtcgt ccgtctacct atgcgtcgat catttcgacc attcaggatc
     5041 gtggctatgt gcgtgtagaa aaccgtcgtt tctatgcgga aaaaatgggc gaaatcgtca
     5101 ccgatcgcct tgaagaaaat ttccgcgagt taatgaacta cgactttacc gcgcagatgg
     5161 aaaacagcct tgaccaggtg gcaaatcacg aagcagagtg gaaagctgta ctggatcact
     5221 tcttctcgga tttcacccag cagttagata aagctgaaaa agatccggaa gaggggggga
     5281 tgcgcccgaa ccagatggtt ctgaccagca tcgactgccc gacctgtggt cgcaaaatgg
     5341 ggattcgcac agcgagcacc ggggtattcc ttggctgttc cggctacgct ctgccaccga
     5401 aagagcgttg caaaaccacc atcaacctgg tgccggaaaa cgaagtgctg aacgtgctgg
     5461 aaggcgaaga tgctgaaacc aacgcgctgc gcgcaaaacg tcgttgcccg aaatgcggca
     5521 cggcgatgga cagctatctc atcgatccga aacgtaagtt gcatgtctgt ggtaataacc
     5581 caacctgcga cggttacgag atcgaagagg gcgaattccg cattaaaggt tatgacggcc
     5641 cgatcgttga gtgtgaaaaa tgtggctctg aaatgcacct gaaaatgggg cgtttcggta
     5701 aatacatggc ctgcactaac gaagagtgta aaaacacgcg taagatttta cgtaacggcg
     5761 aagtggcacc accgaaagaa gatccggtgc cattaccgga gctgccgtgc gaaaaatcag
     5821 atgcttattt cgtgctgcgt gacggtgctg ccggtgtgtt cctggcggcc aataccttcc
     5881 cgaaatcgcg tgaaacacgt gcgccactgg tggaagagct ttatcgcttc cgcgaccgtc
     5941 tgccggaaaa actgcgttat ctggccgatg cgccacagca ggatccggaa ggtaataaga
     6001 ccatggttcg ctttagccgt aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa
     6061 aggcgactgg ctggtcagca ttttatgttg atggcaaatg ggttgaaggg aaaaaataac
     6121 ctttaattct gtcaggtttt tataaacaaa gggtcgcgaa agcggccctt ttttattg
//
LOCUS       DAZUCC010000245         6124 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZUCC010000245 DAZUCC010000000
VERSION     DAZUCC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.246"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            226..2073
                     /gene="ldtD"
                     /locus_tag="ACHY09_004459"
     CDS             226..2073
                     /gene="ldtD"
                     /locus_tag="ACHY09_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS3887336.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVVGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2254..2802
                     /locus_tag="ACHY09_004460"
     CDS             2254..2802
                     /locus_tag="ACHY09_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFS3887337.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2829..3476
                     /gene="gloC"
                     /locus_tag="ACHY09_004461"
     CDS             2829..3476
                     /gene="gloC"
                     /locus_tag="ACHY09_004461"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFS3887338.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3527..4717)
                     /gene="aspC"
                     /locus_tag="ACHY09_004462"
     CDS             complement(3527..4717)
                     /gene="aspC"
                     /locus_tag="ACHY09_004462"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFS3887339.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETIDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4902..5990)
                     /gene="ompF"
                     /locus_tag="ACHY09_004463"
     CDS             complement(4902..5990)
                     /gene="ompF"
                     /locus_tag="ACHY09_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFS3887340.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1531 a         1511 c         1562 g         1520 t
ORIGIN      
        1 cgaaaactcc gtttctgcac taaaaaagtg gcacattacg gcgcgctttt ctttaaactt
       61 ctttacatta ggttatgtaa aaacgtatcg gcgtttatat actgaagata agcctgatga
      121 gtaacaggct tgctcgtcat actttcgtga gtattggcgt tgtacaggca agtcgtaaaa
      181 taacagcctg gctattcaga gtatgataaa aacagggggc aagggatgtt gcttaatatg
      241 atgtgtggtc gtcggctgtc ggcaatcagt ttgtgcctgg ccgtaacatt cgctccactg
      301 ttcaatgcgc aggccgatga gcctgaagta atccctggcg acagcccggt ggctgtcagt
      361 gaacagggcg aggcactgcc gcaggcgcaa gccacggcaa taatggcggg gatccagcca
      421 ttgcctgaag gtgcggctga aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt
      481 tataagccgg tttatcttaa ccagcttcaa ttgttgtatg ccgcacgcga tatgcaaccc
      541 atgtgggaaa accgtgatgc tgttaaagcc ttccagcaac agctggcaga ggtggcgatt
      601 gccggtttcc agccgcagtt taataaatgg gtagagttac tgaccgatcc tggtgttaac
      661 gggatggcac gcgacgtggt gctctctgat gcgatgatgg gctatctcca tttcattgca
      721 aatattccgg tcaaaggcac tcgctggcta tatagcagta aaccttatgc gctttcaacg
      781 ccgccgcttt cggtgattaa ccaatggcag ctggcgctgg ataaaggtca attgcctacg
      841 tttgttgtag gattggcacc gcagcatccg caatatgcgg cgatgcatga atcgttactg
      901 gccttactca gtgacaccaa accgtggccc caactgaccg gcaaagcaac gttgcgccca
      961 ggacagtgga gtaacgacgt accggcgttg cgcgaaatat tgcaacgtac aggcatgttg
     1021 gacgggggac cgaaaattac gctacctggc gatgacacgc caactgacgc ggtagtcagc
     1081 ccatccgctg ttactattga aacagcagaa actaagccga tggacaagca aactacgtct
     1141 cgtagtaaac ctgcgcctgt cgttcgtgcc gcctacgata atgaactggt ggaagccgtt
     1201 aaacgttttc aggcatggca aggattgggg gcagatggtg ctattggccc ggcaacgcgt
     1261 gactggttaa acgtaacgcc cgcccagcgt gctggcgtgt tggcactcaa catccagcga
     1321 ttgcgcttgc tgccaacaga gctttctacc gggatcatgg ttaacattcc agcctattcg
     1381 ctggtctact atcagaacgg caatcaggtg ctggattcgc gagtcattgt cggtcgcccc
     1441 gatcgcaaaa cgccgatgat gagcagtgca cttaacaacg tagtggtaaa cccgccgtgg
     1501 aacgtaccac caactctggc acgcaaagat attctgccga aagtgcgcaa cgatccggga
     1561 tatctcgaaa gccatggcta tacggtgatg cgcggctgga acagcagaga agcgattgac
     1621 ccatggcagg ttgactggtc tacaatcacg gcctcgaatt taccgttccg cttccagcag
     1681 gctccaggcc cacggaactc gctggggcgc tataaattca atatgccgag ttcagaggcc
     1741 atttatttgc atgacacgcc gaaccacaat ctgttcaagc gtgatacacg cgcattgagc
     1801 tcaggctgtg tacgagtgaa taaagcttcc gatctggcga atatgctgtt gcaggatgca
     1861 ggctggaatg acaaacgtat ttctgatgcg ctgaagcagg gcgatacgcg ttacgtcaat
     1921 attcggcagt cgattccggt gaatctctac tatctgacgg cctttgttgg tgcagatggt
     1981 cgtacccagt atcgtacaga tatttacaat tatgatctgc ctgcgcgatc cagctcgcaa
     2041 attgtatcga aagcggaaca attaatcagg taaatgaagc agttcgggga aatcagttgt
     2101 cgtaataatt agcatgattg ggggcaattc tctgcagccc ccgtcactgc tggggttgag
     2161 tcatcttgac gtctgcttta cgggcggtta aggtgcctct tgtgcgccag aagtgcatat
     2221 aaacgataac attgacctgt agacttgatt atcatggaca aattcgacgc taatcgccgc
     2281 aaattgctgg cgcttggtgg cgttgcactc ggtgccgcca tcctgccgac ccctgcgttt
     2341 gcaacactct ctaccccacg cccgcgcatt ttgacactca ataatcttca taccggagag
     2401 tcaatcaaag cggagttttt cgatggcaga ggctatattc aggaagaatt ggcaaaactt
     2461 aaccattttt tccgcgatta ccgcgcgaac aaaataaagt ccatcgaccc aggattattc
     2521 gaccagttgt atcgcctgca agggttgtta ggtacccgca aaccggtgca actcatttcc
     2581 ggttatcgtt ccattgatac caacaatgaa ctacgcgccc gcagccgtgg agtagcgaag
     2641 aaaagctatc acacgaaagg gcaggcgatg gatttccata ttgaaggtat cgcgttaagc
     2701 aatattcgca aagccgcgtt atctatgcgc gcaggtggtg taggatatta cccacgtagt
     2761 aactttgtgc atattgatac cgggccagca cggcactggt agcaattgct taacgaaaca
     2821 ggggcagtat gaactatcgt attattccgg tcaccgcatt ctcccagaac tgttcattga
     2881 tctggtgtga acaaacccgc ctggccgcac tggtcgatcc tggcggcgat gcggaaaaaa
     2941 tcaaacagga agttgacgcc agcggcctga cactgatgca gatcctgctg acgcatggtc
     3001 atctggacca cgttggtgca gcggcggaac tggcgcaaca ttacggcgtg ccggtttttg
     3061 gcccggaaaa agaagatgag ttctggctgc aaggcttgcc tgcgcaaagt cgtatgtttg
     3121 gtctggaaga gtgccagccg ctgacgccgg atcgttggct gaacgaaggc gataccatca
     3181 gcatagggaa tgtggcttta caggtgttac attgccctgg gcatacgccg ggtcatgtcg
     3241 tgttttttga tgatcgggca aagctgctga tttctggcga cgttattttc aaaggcggag
     3301 tagggcgcag tgacttcccg cgtggcgatc ataatcaact gatttcttca atcaaagata
     3361 agttgctgcc actgggggat gacgtgacat ttattccggg tcacggacca ttatccacac
     3421 ttggttatga gcgcctgcat aatcccttcc tgcaagacga aatgcccgtc tggtaaaggc
     3481 gcataaaaaa gcccgctttt taagcgggct tcattgtttt taacgcttac agcactgcca
     3541 caatcgcttc gcacagcgga gccatattat ctggtgtcat cccggccacg tttacgcgac
     3601 cagaagcaac agcatatacg ccaaactctt cgcgcagacg cagcacttgt tcttttgtca
     3661 ggccactgaa ggagaacatg ccgttctgtt tgatgataaa gctgaagtcg cgatttgcgc
     3721 ctttttcctg cagcgtattg acgaacaact gacgcatacg ctgaatacgc tggcgcatat
     3781 cagtcagttc ttgttcccaa atcgcacgta acgcatcgtt gctcaggatg gtggcaacaa
     3841 cagaagcgcc gtgtgctggt gggttagagt agttagcgcg aatcgccgct ttcatttggc
     3901 tgaatgcgcg atcaatggtt tcactgtcag cagcaaccag agtacaagcg ccaacacgct
     3961 cgttgtacag gccaaagttt ttagagtagg aactggcaac aatcagctct ttatgcatag
     4021 ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc acgggcaaac ccctggtaag
     4081 cgaagtcaaa cagcggtaac cagcctttct caacggaaag ttgtgccagt gtctgccatt
     4141 gttccagcgt agggtcgata ccggttgggt tatggcagca gccatggaac agcaccacgt
     4201 cgccagcctg agcttcgttc aggctgttaa tcagtgcatc gaagtcaagg gtgtgatttt
     4261 ccgcatcata ataagcgtat tcacgaactt ccagacctgc agagttaaag acgctcttat
     4321 ggttcggcca gcttgggttg ctcacccaca cacgcttaac gctggtattt tttgccagga
     4381 aatcggcagc cacgcgtagt gcgccagtgc cacccggagt ctgtgccgtg cgagcacgtt
     4441 tgtcattgat cagggcgcta cctttaccaa acagcagttc ctgagtgcag cgaccaaatt
     4501 cagggatgcc gtcaatgccg aggtaatttt tggtggtttc attttcgagc agatactgtt
     4561 cagccttttt cacactggtc agtaccgggg ttttgcccgt ctcatcttta tagacaccaa
     4621 tcccgaggtt aattttgccg ggacgttcat cggcacgaaa cagatcggcc aggcccagaa
     4681 tcgggtcggc aggagcggcg gtaatgttct caaacatgac gaggttccat tatggttaca
     4741 gaagggaagt ccgctatcag ggtaacggga gatttacaaa attccaacta ttactgatga
     4801 aaacgcaggc tgtttttgca agacgtgaga ttgctctgga aggcataaaa aaaacaggac
     4861 caaagtcctg ttttttcggc atttaacaaa gaggtgtgct attagaactg gtaaacgata
     4921 cccacagcaa cggtgtcgtc agaacctacg cccagtttgt tgtcagaatc gatctggttg
     4981 atgatgtagt caacataggt ggacatgttt ttgttgaagt agtaggttgc gcccacttca
     5041 aagtagttaa ccagatcaac atcaccgata ccttctacgt ctttcgcttt agatttggtg
     5101 taagcgatgg acggacgcag accgaaatcg aactggtatt gcgcaactaa cagaacgtct
     5161 tgcgttttgt tggcgaagcc gctgatgttt gtaaatttat tagtgatcgg cgtagcgtta
     5221 cgggtttcac cgtagttcgc tgccaggtag atgttgttcg cgtcatactt cagaccagta
     5281 gcccactgtt cagctttttt accgttgcca agaggttgag cttcttgcag gttggtacgg
     5341 tcagcggcgc cataagcacc aacgatacca aagccttcgt attcgtagct gatagaaccg
     5401 ccaacaccgt cgccgttaga gcggcgtgca gtgtcacgct cgtttttacc caggtactga
     5461 acagcgaagt tcaggccatc aaccagacca aagaagttgg agttacgata ggtagcaacg
     5521 ccgccaacac gaccaacgaa gaagtcatcg ctgtatgccg tgtcaccgcc aaactcaggc
     5581 agcatatcgg tgtaacccag tgcatcataa accacaccgt agttacggcc gtaatcgaaa
     5641 gaaccaacgt cagcgtattt aagacccgcg aatgccagac gcgttttgtt accagtttga
     5701 gcgtcagcgc cttcagagtt gttaccctgg aagttatatt cccactgacc ataaccggtc
     5761 agatcggaat tgatttgagt ttccccttta aaaccaagac gggcataggt catgtcacca
     5821 ttgccaccgt aactgttttc accgttaccc ttggagaaat aatgcagacc gacagcttta
     5881 ccgtacagat ctactttgtt gccatctttg ttatagattt ctgcagcgtt tgcagtacct
     5941 gctactaaca gagcagggac gatcactgcc agaatattgc gcttcatcat tatttattac
     6001 cctcatggtt tttttatgac acctgccact gccgtcaata agttctgtca ataaaaattt
     6061 acggaactat tgatgagagt ttggtgtctt tatgtgtctg caggcatctt tccattcaaa
     6121 ctaa
//
LOCUS       DAZUCC010000246         6111 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZUCC010000246 DAZUCC010000000
VERSION     DAZUCC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.247"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..375)
                     /locus_tag="ACHY09_004464"
     CDS             complement(<1..375)
                     /locus_tag="ACHY09_004464"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3887341.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELK"
     gene            1166..1336
                     /locus_tag="ACHY09_004465"
                     /pseudo
     CDS             1166..1336
                     /locus_tag="ACHY09_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1552..2385)
                     /locus_tag="ACHY09_004466"
     CDS             complement(1552..2385)
                     /locus_tag="ACHY09_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS3887342.1"
                     /translation="MMKLGFNEATCLRNSSLEKDLVLAERFGYDYIEIRLDMLQTWLQ
                     EHTLVELADIFSVSHLKPWGYNSLEDITFCDAGRWSEIRQQLVLACYAGEVVGGDCLV
                     VVPTIREGAHFSDRDTVRDSVKRLREMSALAAESQMRLAFEPIGSAGCCVRSLAMAME
                     IVDAVDRSNVGLAIDAFNLYLYDQWQDLSTLRQVPVEKIFVYHIDDADRLPVAVLDHC
                     HRLFPGNGAIPLYMITHELVKKGYTGICSLELFNPGYWQMAASEVFAIGAEKTRPFLA
                     D"
     gene            complement(2392..3729)
                     /locus_tag="ACHY09_004467"
     CDS             complement(2392..3729)
                     /locus_tag="ACHY09_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HFS3887343.1"
                     /translation="MKGRLITSDPWNQQFMVELEVFFSPRQPACSELISVFPNHSLQQ
                     IDGFGGSFTESAGVVFNSMSDKTKAQFLNLYFSPQEHNYSLGRMPIQSCDFSLSNYAY
                     VDSSEDLRQGRISFSRDESHLMPLISAALRINPQIKLMASPWSPPAFMKTNYDMNGGG
                     KLRYEYYADWADIIIDYLLEYRRHGISVKALSVQNEPVAIKSWDSCLYSVEEETTFAV
                     HYLRPRLARYGMNEIEIYIWDHDKDGLIDWAERAFADEKDGDSINGMAFHWYTGDHFS
                     QIQYLARKLPNKKLLFSEGCVPMENDEGGQIRHWHTYIHDMIGNFKSGCSGFIDWNLL
                     LDSDGGPNHQSNLCEAPVQYDALNDILWCNHSWYGIGHFCRFVHPGAKVMLTSSYDTQ
                     LEDVGFVNPDGERVLVVYNRDTRERHFRVVDGDRELALTLPPSGASTLVWRQE"
     gene            complement(3827..4837)
                     /gene="iolG1"
                     /locus_tag="ACHY09_004468"
     CDS             complement(3827..4837)
                     /gene="iolG1"
                     /locus_tag="ACHY09_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase IolG1"
                     /protein_id="HFS3887344.1"
                     /translation="MTLKAGIVGIGKIGADHLRRLANTISGVEVVAVCDIVEGRAQAA
                     LDKYEIKAKDYKDYHDLINDEDVEVVVITASNEVHADVAVAALNANKYVFCEKPLAVT
                     ADDCQRVIGAERKNGKRMVQVGFMRRYDKGYVQLKNIIDSGEIGQPLMVHGRHYNVST
                     VSDYKTPQAIYETLIHEIDVMHWLLNEDYRTVKVYFPRQSSLVTTLRDPQVVVMETTS
                     GINIVVEVFVNCQYGYDIHCDVTCEKGMVELPTVATAAVRKEAKYSTDILVDWKQRFI
                     DAYDIEFQDFFDRLNAGLPLAGPTSWDGYLAAVTADACVKSQETGNTEFVELPAKPDF
                     YK"
     gene            complement(4855..5760)
                     /gene="iolE"
                     /locus_tag="ACHY09_004469"
     CDS             complement(4855..5760)
                     /gene="iolE"
                     /locus_tag="ACHY09_004469"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFS3887345.1"
                     /translation="MYSVKESIKWGIAPIGWRNDDLPEIGKDNTYKQILSDAALTGFS
                     GTEVGGCYPLDPTELNIELALRHLEIPGKWFSSFIIRDGIESVKKAFEQHCAYLQAVH
                     AYIAVVSEQTYSIQGIIDKCVYTEKPYFNDIEWQQLCSGLNELGMIANTYGLKLAFHH
                     HMGTGVQTLQEVDRLMENTDPQLVNLLFDTGHIYVSDGDVMSLLCKHFSRIKHVHFKD
                     VRDEKLKACRAGKKSFLNSFLDGMFTVPGDGNIDFKSVLAYLVENHYSGWIVVEAEQD
                     PNIYNPLEYAQKGKKHIDGLLGNYI"
BASE COUNT         1745 a         1498 c         1186 g         1682 t
ORIGIN      
        1 cttcagttcc tgtatcagca gagtcaggag cttttcgctg acattgccat tgaagcggag
       61 cgtcccgtgc cggaacgtta cctcacagct gatactgagg gtttccgggt cctctgcgag
      121 cgattctggc tgttcggcag ctgcatcgag agtcacagga agtagctggg ggctctctga
      181 agaaggtaat agcagctttc cctcgcgcca ttgttgtcgc catttgaaca acagattggc
      241 gttaatgcca ttttcaagag caagttttga gatggatatc ccgggctcac aggaggcagc
      301 aacgagctgc tgtttaaatt cgggaggata attagggcag ccttttcgcc tgccgggagt
      361 cacatttttc tgcatatctg acactttggt tcccactact tatttggtgg ataccacttt
      421 gtctaattcg tcagattctg accagacggt tcaggctgta cgcttacctt taactggttg
      481 ctgatataag tttttcacca cgcataaaca ctgcccatgc gatcctggca atcttatttg
      541 ccagctctat gatgacgata ttcgttggtc gacgtgcctg ctgggcttaa atctattccc
      601 ccggggatcc ggctttattg caaccataaa gctgcgagcc cctatgtaat tcgctgactc
      661 acacctcatt tactgcgcgg gattcaagcc caaaacccca tttaggttta gtgagtccct
      721 gtcttacctg tctttgtcat cacgttgacc accagagcaa cctttctgca ttcacccatt
      781 taagtaacca aaatgagtat caggcctgat atatacagaa ctgaattcgt taattaatta
      841 ttaatcagtt aaattttccg ttactgggat gaggtgtaca cttggcctct cccaaaatcc
      901 gcggaaccgc ccctggcccc tattcagcat ttcaggctct aagttgagga ctgaccggat
      961 tgacataggg cctgctgacg ccattcattg actaccaaca gatctttctt gttatttata
     1021 gaaaaccacc agccagccta gtggttcagt aaaacttcca actttgaggc tgttgtcaaa
     1081 accccttttt gatttgttaa aacaccttgc agtctaccaa ctgtaagcgt caaacaagaa
     1141 atcaaaaggg gattccaatg ggtgaatgaa aaagggctta acgaacaccc gatggaactg
     1201 taaatatcac atagtttttg cgccaaaata ccgaagacag atgttctaca gagaaaagcg
     1261 aagtgcaaga gcaccaggca atattttgag aaaactgtgt gagtggaaaa gtgtacggat
     1321 tctggaagct gaatgctgag cggatcatat ccatatgctt gtgaatttac ggggcgtaag
     1381 taaggaagtt gaatgaaaat gtcagatcat atgcgcctgt cagccagggc gaggcagaaa
     1441 acgaatcttt aagacacatc tgaaaaacct ccggctatgc cggaggagat ttattctgat
     1501 cacctgctta ttaccgggca cagcgacagt tcatttgcag ttacgtcagc gttaatccgc
     1561 taagaaaggt cgcgtttttt ccgctccgat agcaaagacc tcgctggctg ccatttgcca
     1621 gtaacctggg ttaaacaact ccagtgaaca gatccctgta taaccttttt tcaccagttc
     1681 atgggtgatc atgtacagtg gtatcgcacc gttgcctgga aacagacgat gacaatgatc
     1741 caacacagct acaggtaaac ggtcagcatc atcaatatga tagacaaaga tcttttccac
     1801 cggaacctgt cgcagcgtac tcagatcctg ccattgatcg tacagataaa ggttaaaggc
     1861 atctattgcc agaccaacgt tactccgatc aactgcgtcc acgatctcca tcgccatcgc
     1921 cagactgcgt acgcaacatc ccgcagagcc gataggctca aaggccagtc gcatctggct
     1981 ctctgccgct aatgctgaca tctcacgcaa acgtttcact gaatctctta ccgtgtcacg
     2041 atctgaaaaa tgagcacctt cacgaatggt cggcactaca accaggcaat caccacctac
     2101 gacttcccct gcataacagg ctaaaaccag ttgttgccgt atttccgacc agcgcccggc
     2161 atcacaaaat gtgatgtctt ccagcgagtt gtagccccat ggcttaagat ggcttacaga
     2221 aaaaatatcg gccagttcaa ccagagtgtg ttcctgcagc cacgtctgca acatatccag
     2281 acggatctca atatagtcat atccaaaacg ttccgctagg accagatcct tctccaggct
     2341 ggaattacgc agacaggtcg cttcattgaa tcccagtttc atcattcaga cttactcctg
     2401 ccgccatacc aatgtactgg ctccagaagg cggcagcgtt aatgcgagtt ctctgtcgcc
     2461 atcgaccacc cggaaatgcc tttctcgcgt atcgcgatta tataccacta aaactcgctc
     2521 accatcagga ttaacaaaac ccacatcttc cagttgcgta tcataactgg aagtaagcat
     2581 aactttcgca ccgggatgca caaagcggca aaagtgtcca attccgtacc aagagtggtt
     2641 acaccagaga atgtcattca atgcatcgta ctgcacagga gcttcgcaca aattactctg
     2701 gtgatttggg cctccatcac tgtctaacag caggttccag tcgataaatc cgctacagcc
     2761 ggatttaaaa ttaccaatca tgtcatgaat ataggtatgc cagtgacgga tctggccacc
     2821 ttcatcattc tccattggta cacatccttc ggaaaacagc agctttttat tcggcaactt
     2881 ccgggccaga tactgaattt gcgaaaaatg gtcaccggta taccagtgga aagccatccc
     2941 gttgatgcta tcgccatcct tttcgtcagc aaaggcacgt tcagcccagt caatcagacc
     3001 atctttatca tgatcccaga tatagatctc tatttcattc attccatagc gggcgagtcg
     3061 tgggcgcaga tagtgaacag caaaagtagt ctcttcttct acactataca gacaggaatc
     3121 ccagcttttt atcgcaactg gttcattctg cactgatagt gctttaacac tgataccatg
     3181 gcgtctgtat tcgagcagat agtcgataat aatatctgcc cagtcagcat agtattcata
     3241 ccgcaatttc ccacctccgt tcatatcata attagtcttc atgaatgccg gtggactcca
     3301 cggggaagcc attaatttta tttgtggatt gatccgcaat gccgcggaaa ttagcggcat
     3361 taaatgagat tcatcacggg aaaaggaaat ccttccctga cgcaggtctt cactggaatc
     3421 aacataggca taattgctca gagagaaatc acagctttgg atcggcatcc gccccaaact
     3481 atagttatgt tcctgaggcg aaaaatagag attcaaaaat tgcgcctttg tcttatcact
     3541 catgctgttg aaaacaacac cagcactttc agtgaagctg ccgccaaatc catcaatctg
     3601 ttgtaacgag tggtttggaa agacgctgat taattcacta cacgctggtt gacgaggcga
     3661 aaaaaaaacc tccagctcaa ccataaattg ctgattccag ggatcggaag tgattagtct
     3721 gcctttcata gagggattcc agataatata ggatgataaa ttgtgatata ccggacagga
     3781 gcatacacct agctatccgg tatgaaatta tcacatcgca gaaagactat ttgtagaagt
     3841 caggttttgc tggtaattcc acaaactcgg tgtttcctgt ttcctgggac ttcacacagg
     3901 catcagccgt taccgcagcc agataaccat cccatgatgt tgggcctgcc agaggtaatc
     3961 ccgcattcag acggtcaaag aagtcctgga actcaatatc ataggcatca ataaagcgtt
     4021 gtttccagtc caccaaaatg tcagtactgt attttgcctc tttacgcacc gcagcagtag
     4081 ccacggtagg taattccacc atccctttct cacaggtcac atcacaatga atatcatagc
     4141 catactggca attcacaaac acctcaacca ctatattaat accggaggtc gtttccatca
     4201 ccaccacctg cggatctcgt agagtagtca ccaagctgga ctgacgtggg aagtagacct
     4261 taactgttct ataatcttcg ttgagtaacc agtgcataac atcgatttca tgaatcagcg
     4321 tttcataaat tgcttgtggt gttttgtaat ccgataccgt gcttacatta taatgacgac
     4381 catgaaccat taatggttga ccaatttctc cgctatcgat gatgtttttc agctgtacgt
     4441 agcctttatc gtagcgacgc ataaacccaa cttgcaccat tcgttttcca tttttacgtt
     4501 cagctccgat cacacgctga caatcatctg ctgtaaccgc aagcggcttt tcacagaaaa
     4561 catatttatt ggcatttaac gctgcaacgg ctacatcggc atgtacttca ttcgaggcag
     4621 taattaccac tacttcgaca tcttcatcat taatcagatc gtgatagtct ttatagtctt
     4681 ttgctttgat ctcatactta tccaaagctg cttgcgctct gccttcgaca atatcgcaca
     4741 cagcaacaac ttctactcca gaaatagtgt tagccagacg tcttaagtga tcggcaccaa
     4801 ttttcccgat ccccacaata cctgctttta aagtcatctt attttccttt tctatcagat
     4861 ataattaccc aataaaccat caatatgctt tttacctttt tgtgcatatt ccaaaggatt
     4921 atatatattc gggtcctgct cagcttcaac gacaatccag ccggaataat gattctcaac
     4981 caaataagcc aatacagatt taaaatcaat attcccatca ccaggaacag taaacattcc
     5041 atcaagaaaa gaattgagaa atgatttttt tccagcccga catgctttta gtttttcatc
     5101 ccgaacatct ttaaagtgaa catgtttaat acgggaaaaa tgcttgcaca acagtgacat
     5161 cacatctcca tcagaaacat aaatatgacc agtatcaaac agcaaattca ctaattgtgg
     5221 atctgtgttt tccataagtc gatccacttc ctgtaaagtt tgtacgccag tgcccatatg
     5281 atggtggaaa gcaagtttaa gcccatatgt attggcgatc atgcctaact cgttcaatcc
     5341 tgagcataat tgttgccatt cgatatcatt gaagtaaggt ttttccgtat agacacattt
     5401 atcaataatt ccttgaatac tatacgtttg ctcagacaca acagcgatat aagcatggac
     5461 agcctgcaaa taagcgcaat gttgttcaaa agctttcttc actgattcaa taccatcacg
     5521 aataataaaa gatgagaacc actttccggg aatttccaga tggcgaagtg ctaactctat
     5581 atttaattca gtcgggtcca gaggataaca tccccccact tctgttccag aaaaaccagt
     5641 aagtgctgca tcacttaata tctgcttata ggtattgtct ttaccaattt caggcaaatc
     5701 atcatttcgc catccgattg gcgcaatccc ccatttaata ctctctttta cactatacat
     5761 attccagact cctgcttaat aaaactttct cacatcctgt tgcataagta ataatctttg
     5821 gctgacaata tgctactttc cacgtggcta aaaaacttta tctatcatga gtacagaagt
     5881 gggaaaaatg tgtcaggaat cacaaaaaaa acacaaccct ttaatgattt tgtctatgtt
     5941 ttagtcatgc tgaaaaatca cacaatgacc gtaaaataaa accgtcactt tcccactaac
     6001 tctggcgtgg acagaatcat cccgtatgat tcccaagttt ggtaaatatt attaaatcag
     6061 gatgtgcaag ctatgattca gtcatactag gaaaaaagtt tttttgcaga t
//
LOCUS       DAZUCC010000247         6092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZUCC010000247 DAZUCC010000000
VERSION     DAZUCC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.248"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            212..1342
                     /gene="ompC"
                     /locus_tag="ACHY09_004470"
     CDS             212..1342
                     /gene="ompC"
                     /locus_tag="ACHY09_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFS3887346.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNGTYVSDNNVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1454..2509
                     /gene="apbE"
                     /locus_tag="ACHY09_004471"
     CDS             1454..2509
                     /gene="apbE"
                     /locus_tag="ACHY09_004471"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFS3887347.1"
                     /translation="MEISFTRVALLAAALFFVGCDQQPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            2583..3647
                     /gene="ada"
                     /locus_tag="ACHY09_004472"
     CDS             2583..3647
                     /gene="ada"
                     /locus_tag="ACHY09_004472"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFS3887348.1"
                     /translation="MKYATCLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSIINAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVRDVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3647..4297
                     /gene="alkB"
                     /locus_tag="ACHY09_004473"
     CDS             3647..4297
                     /gene="alkB"
                     /locus_tag="ACHY09_004473"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFS3887349.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4373..6016
                     /gene="yojI"
                     /locus_tag="ACHY09_004474"
     CDS             4373..6016
                     /gene="yojI"
                     /locus_tag="ACHY09_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFS3887350.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1452 a         1536 c         1635 g         1469 t
ORIGIN      
        1 attccgcaat aatcttaaaa agttcccttt catttacatt ctgaaacatc tatagcgata
       61 aatgaaacat cttaaaagtt ttagtatcat attcgtgttg gattattctg catttttggg
      121 gagaatggac ttgccgactg attaatgagg gttaatcagt atgcagtggc ataaaaaagc
      181 aaataaaggc atataacaga gggttaataa catgaaagtt aaagtactgt ccctcctggt
      241 cccagctctg ctggtagcag gcgcagcaaa cgctgctgaa gtttacaaca aagacggcaa
      301 caaattagat ctgtacggta aagtagacgg cctgcactat ttctctgacg acaagtctgt
      361 agatggcgac cagacctaca tgcgtcttgg cttcaaaggt gaaactcagg ttactgacca
      421 actgaccggt tacggccagt gggaatatca gatccagggc aacagcgctg aaaacgaaaa
      481 caactcctgg acccgtgtgg cattcgcagg tctgaaattc caggatgtgg gttctttcga
      541 ctacggtcgt aactacggcg ttgtttatga cgtaacttcc tggaccgacg tcctgccaga
      601 attcggtggc gacacttacg gttctgacaa cttcatgcag cagcgtggta acggcttcgc
      661 gacctaccgt aacaccgact tcttcggtct ggttgacggc ctgaactttg ctgttcagta
      721 ccagggtaaa aacggtagcg tagatggcga aggcatgacc aacaacggtc gtggtgctct
      781 gcgtcagaac ggcgacggcg ttggcggttc tatcacttat gattacgaag gcttcggtat
      841 cggtgctgca gtgtccagct ccaaacgtac tgacgctcag aatggtacct atgtaagcga
      901 caataatgtg gtccgtaact acatcggtac tggtgaccgt gctgaaacct acactggtgg
      961 tctgaaatac gacgctaaca acatctacct ggctgctcag tacacccaga cctacaacgc
     1021 aactcgcgta ggttccctgg gttgggcgaa caaagcacag aacttcgaag ctgttgctca
     1081 gtaccagttc gacttcggtc tgcgtccgtc cctggcttac ctgcagtcta aaggtaaaaa
     1141 cctgggtgtc atcaatggtc gtaactacga cgacgaagat atcctgaaat atgttgatgt
     1201 tggtgcgacc tactacttca acaaaaacat gtccacctat gttgactaca aaatcaacct
     1261 gctggacgac aaccagttca ctcgtgacgc tggcatcaac actgataaca tcgtagctct
     1321 gggtctggtt taccagttct aatctcgatt gatatcgaac aaagggcctg cgggcccttt
     1381 tttcattgtt ttcagcgtac aaacacagtt ttttggtgta ctcttgcgac cgttcgcatg
     1441 aggataatca cgtatggaaa taagctttac ccgcgtggca ctgctggctg ccgcgctctt
     1501 ctttgttggt tgcgatcaac aaccacaacc cgccaaaacc cacgctactg aagttaccgt
     1561 tcttgaaggc aaaaccatgg gtaccttctg gcgtgccagc atcccgggca ttgacgccaa
     1621 acgcagtgcc gaacttaaag aaaagattca gacccaactg gacgctgacg atcagctgct
     1681 ttcgacctat aaaaaagatt ccgcgctgat gcgctttaac gactcgcaaa gtttgtcgcc
     1741 gtggccggta agtgaagcga tggccgatat cgtcactacc tcgctgcgca ttggcgcgaa
     1801 gaccgatggc gcgatggata taaccgtcgg gccgctggtg aatctgtggg gctttggtcc
     1861 ggaacaacaa ccggttcaaa ttccgagcca ggaacagatc gatgcgatga aagccaaaac
     1921 cggcttacag cacctgacgg tcattaatca gtcgcatcag caatatctgc aaaaagacct
     1981 gccggattta tatgtcgatc tctccaccgt cggcgaaggt tatgcggcgg atcacctggc
     2041 acgcttgatg gagcaggaag ggatttcccg ctatctggtg tcggtgggcg gcgcgctgaa
     2101 cagccgtggt atgaacggtg ctggcaagcc gtggcgggta gcgattcaaa agccaaccga
     2161 taaagaaaac gcggttcagg ccgtggtgga tatcaacggt catgggatta gtacctctgg
     2221 cagctaccgt aactattacg aactggacgg caaacgtctt tctcatgtta tcgatccaca
     2281 aaccgggcgt cctatcgaac acaatctggt atccgtgacg gtgattgccc cgacggctct
     2341 ggaagccgat gcctgggata ctggcttaat ggtactcgga ccggagaaag ccaaagaggt
     2401 tgttcgccgg gaaggactgg cggtctacat gatcaccaaa gagggcgata attttaaaac
     2461 ctggatgtcg ccgcagttta aaagcttcct tatcagcgaa aaaaattaaa gcgcaagatt
     2521 gttggttttt gtgcgatggt gaccgggcag actaaaggct atccttaacc agggagctga
     2581 ttatgaaata tgccacatgc ttaactgacg atcaacgctg gcaatctgtc ttagcccgcg
     2641 actcgaatgc cgacggcgaa ttcgttttcg ccgtgcgcac cacgggtatc ttttgccgtc
     2701 cgtcttgccg agccagacat gccttgcggg aaaatgtctc cttctacgca aatgccagcg
     2761 aggcgcttgc cgctggcttt cgtccctgca aacgttgtca gccagacaaa gccaatcccc
     2821 tgcaacatcg ccttgataaa attacccacg cctgccgact tctggaacag gaaacgcctg
     2881 taacgctgga agccttagcc gaccaggtgg cgatgagtcc atttcatttg catcggttgt
     2941 ttaaagcgac taccggaatg acgcctaaag cctggcaaca ggcctggcgc gctcgccgtt
     3001 tgcgcgaatt gctggcgaaa ggggagagcg tgactacatc tattattaac gccggattcc
     3061 ccgacagcag cagttactac cgcaaagccg acgaaacgct gggcatgacg gctaaacaat
     3121 tccgtcacgg tggcgaaaat ctggcggtgc gttacgcgct ggctgattgt gagctgggcc
     3181 gttgcctggt ggcagaaagt gagcggggga tttgcgcgat attactgggc gatgatgacg
     3241 cgacactaat cagcgagtta cagcagatgt ttcccgctgc cgacagcgcg cctgctgatc
     3301 ttacgtttca gcaacatgtg cgtgacgtga tcgccagcct caatcaacgc gatacgccgc
     3361 tgacgttacc gctggacatt cgcggcactg cttttcagca acaagtctgg caggcactgc
     3421 gcacgatacc ttgcggtgaa accgtcagtt atcagcaact ggctaatgcc atcggcaaac
     3481 cgaaagcggt acgggccgtt gccagcgcct gtgccgccaa caagctggct atcgtaatac
     3541 cctgtcatcg ggtggtccgt ggtgatggca cactttccgg ttaccgctgg ggcgtgtcgc
     3601 gcaaagcgca actgctgcgc cgcgaagctg aaaatgagga aaggtaatgt tggatctgtt
     3661 tgccgatgct gaaccgtggc aagaaccact ggcggctgga gcggtgattt tgcaccgttt
     3721 tgcttttaac gctgcggagc aactgatccg cgatattaat gacgttgcca gccagtcgcc
     3781 gtttcgccag atggtcactc caggcggata taccatgtcg gtggcgatga ccaactgtgg
     3841 gcatctgggc tggacgaccc atcggcaagg ttatctctat tcgcccattg atccgcaaac
     3901 aaataaaccg tggcccgcca tgccgcagag ttttcatcat ttatgtcaac gtgcggctac
     3961 ggcggcgggc tatccagatt tccagccaga tgcctgtttg atcaaccgct acgctcctgg
     4021 cgcgaaactg tcgctgcatc aggataaaga cgaaccggat ctgcgcgcgc cgattgtttc
     4081 tgtttctctg ggcttacccg cgatttttca atttggcggc ctgaaacgaa atgatccgct
     4141 aaaaagattg ttgctggaac atggcgatgt ggtggtatgg ggcggtgaat cgcggctgtt
     4201 ttatcacggt attcaaccgt tgaaagcggg gtttcatcca ctcaccaccg actgccgcta
     4261 caacctgaca ttccgtcagg caggtaaaaa agaataaaaa taagaattat tattgctgtg
     4321 cgcgcgaaga tgtttaaact gcgggctgta attcattgtc cgggttttct gcatggaact
     4381 tcttgtactt gtctggcggc agtatcgctg gccatttatc agtgtgatgg cgctaagcct
     4441 cgccagtgcg gcattaggca ttggcttaat tgcttttatc aatcagcgcc ttatcgaaac
     4501 ggcggatacc agtctgctgg tattgccgga gtttctgggg ttattgctgc tgttgatggc
     4561 cgtcactctc ggatcgcaac tggcgctcac cactttgggg catcacttcg tttaccgact
     4621 gcgtagtgaa tttatcaagc ggattctcga tactcacgtc gagcgcattg aacaactcgg
     4681 tagcgcctcg ttgctggcgg gattaaccag cgatgtgcgc aatatcacca ttgcttttgt
     4741 gcgtctgccg gaactggtgc aggggatcat tctcactatc ggttcagcgg cgtatttgtg
     4801 gatgctgtcg ggcaaaatgt tgctggtaac ggctatctgg atggcagtca ccatctgggg
     4861 cggttttgtg ctggtggcgc gggtgtacaa acatatggcg accctgcgtg aaaccgaaga
     4921 taagctgtac acggattttc aaactgtact cgaagggcgc aaagagctga ctctgaaccg
     4981 tgaacgcgcc gagtatgtgt ttaacaacct ctacattcct gatgcgcaag agtatcgcca
     5041 tcatattatt cgcgcagaca ccttccatct tagtgccgtg aactggtcaa acatcatgat
     5101 gctgggcgct atcggcctgg tgttctggat ggcgaacagt ctcggttggg ctgataccaa
     5161 cgttgctgcg acctattcgt tgacgctgtt attcctgcgt acgccgctgc tttcggcggt
     5221 tggcgcattg ccgacgctgc tgacggcgca ggtggcgttt aacaagctga acaaattcgc
     5281 gctcgcgcct ttcaaagcag agtttccgcg cccgcaggct tttcccaact ggcaaacgct
     5341 ggagctgcgt aacgtgacgt ttagctatca ggataacgcg ttttccgttg ggccaattaa
     5401 cctcaccatc aaacgtggcg agctgctgtt tctgattggt ggcaacggta gtggaaaatc
     5461 gacgctggcg atgttgctga cgggcttgta ccagccacaa aatggcgaaa tcttgctgga
     5521 tggcaaacct gtcagcggcg aacaaccgga agattatcga aaactgtttt cggcagtgtt
     5581 taccgatgtc tggctgtttg atcaactgct ggggccggag gggcaacccg ctaacccgca
     5641 actggttgag aagtggctgg cgcagctgaa aatggctcat aagcttgagt taagcaacgg
     5701 gcgtattgtt aacctgaagt tatcaaaagg gcagaaaaaa cgcgtggcgc tgttgctggc
     5761 gctggcagaa gaacgcgata ttatcctgct ggatgaatgg gcggcggatc aggatccaca
     5821 cttccgtcgt gagttttatc aggtgctgct gccgctgatg caggagatgg gtaaaactat
     5881 tttcgccatc agtcatgatg atcattactt tatccacgcc gaccgcctgc tggaaatgcg
     5941 caacgggcaa cttagcgagc tgacaggcga agagcgcgat gctgcttcgc gtgatgccgt
     6001 tgcccggacg gcataatatt acgacgctga cctggcggac tgctgccgcc aggttaatat
     6061 gctaaaaatc ccttcatttc taataaccct at
//
LOCUS       DAZUCC010000248         5946 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZUCC010000248 DAZUCC010000000
VERSION     DAZUCC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.249"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            136..412
                     /locus_tag="ACHY09_004475"
                     /pseudo
     CDS             136..412
                     /locus_tag="ACHY09_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(521..4036)
                     /locus_tag="ACHY09_004476"
     CDS             complement(521..4036)
                     /locus_tag="ACHY09_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFS3887351.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTIHTSFSIDFDDSVDKTAETD
                     EEREARYAALQQEWRQYLDDSERLLKNVAGDWIKNPEQYELAEHGYIVKTAQSGGASF
                     HILSLYDHLLVCKKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGTGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPEKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     VRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRSKRQFQIQLFLEDKLGALIAGNQWSDPETIERNIDELLNSAECEQTTYRKQIDSA
                     HEIFLKEQQAVQEWQRLALDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQEEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGRENSNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAAWLAENQQNIEAHYGKSLHEVIGIVTPFSAQVSTIKQALGKQGISTGANEKSL
                     TVGTVHSLQGAERAIVVFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPPSSPRGLLAKYLFESEKNALSFDYKERKDLKTSGTKIYTLHGVEQHDNFLNQTF
                     ENTSKYITIVSPWLTWQKLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHKDYEKRKE
                     KQQNLKGTLEKLNAFGITTKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLEKRQV"
     gene            complement(4488..5750)
                     /locus_tag="ACHY09_004477"
     CDS             complement(4488..5750)
                     /locus_tag="ACHY09_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3887352.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRINS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAERKPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TREVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKINNSLK"
BASE COUNT         1545 a         1435 c         1290 g         1676 t
ORIGIN      
        1 atttatatac aagtaaaaac aagtaattca aaatttattt tttgaaataa ataatgtggt
       61 atgataataa atccaggtca gcagtaactg gctatcgtgt ataattatcc agaagtcctg
      121 ctgagccttc cgtctgttca tcatgtgcca ggtttgcgtt cgggatatga tgtgccatct
      181 tatgatgatg gcaagagata ttactgcgta ccacctcaat tctgtacaca tcagtgcccg
      241 tcgtttcccc gggatttttc agtgggccac cggtattcgt accgaaaata taccgccatt
      301 caatccgctg acagacgaag ataatgaacc cgttatggtt tttaacggag agaaaaatgt
      361 gccggcagac agtctgaaac cgcagtcttt gattcttccg gacagaagat aatcatgttg
      421 gtatgaatgt tataattaag gactgtgcaa taatagctat tccattcagc catcttacgc
      481 tcgaaaacga tcgataagat ggctactcac cattcctgct ctaaacctga cgcttctcaa
      541 gactattata aatagcctca atctcaccct tcaggttatc accgcaataa accattgagg
      601 tatcgtatcg ttcatatcgc gcttctcgcg ttgcgctaaa ccagttgaac gatcccacgc
      661 acagcaaacc atcatcacca ataacaattt tgctatgaac gcgatttacc agctttgtag
      721 taataccaaa agcattcagt ttttccagcg tccctttaag attctgctgc ttctcttttc
      781 gtttctcata atctttatgt tcagtgttgt agcttctgtc agtgactatc gtgacgttaa
      841 taccacgtga acacgccgca atcatggaat caagaaaacc ggtttgctcc agcttttgcc
      901 aggtcagcca tggagaaact atcgtgatgt atttactggt attttcaaat gtctgattca
      961 ggaaattatc atgttgctcc acaccatgaa gtgtgtagat tttggtcccg gaggttttta
     1021 aatccttacg ctctttataa tcaaaagaga gcgcattctt ctctgactca aagaggtatt
     1081 ttgccagcaa tcctcgcgga gatgagggcg gctggacctc aaacagatcc atatcgccga
     1141 agaccaggaa gctatccttc gcacgggaga ctgcaacatt cagcatgctg ttatcgctat
     1201 caataaaccc gccgtcttca tgttttgaat agactggcga gaataccaca atcgctcttt
     1261 ccgctccctg aagagagtgc acggtgccca ctgtgagcga cttttcattc gcgccagtac
     1321 tgataccttg tttacccagc gcctgtttga tagtggatac ctgcgcgcta aaaggcgtca
     1381 cgataccgat aacttcatga agcgatttgc cgtaatgcgc ttcaatattt tgctggttct
     1441 ctgccaacca ggccgctatt gtttcagcct caagcaaatt atatcgactt ccactacttg
     1501 ccagctctcc tttaccatca atatggagat accccattgc gggcattaaa ttgctatttt
     1561 cacgccctct tttaggcaac aacttaccgt gatagcagag cgtattacag tatccaataa
     1621 tattgtcgta gcaccggcgg tgttcatata ggtacatacc acgagccagt tcgggatcat
     1681 attgatagcg cgaagcaaac tgcgctattt tcataacgct gccagatgcg gcacttttac
     1741 caaggtctgc gattgccgta tatttctcgg taatctcttc ttgcgtactg ccagacagaa
     1801 ttttttccgc aagcatgtta ccgacatcaa tcgcgggagc aatactccat attggcggga
     1861 tctgctccgt atcgccaatc actaatgcct tcttagctaa tgcaaacgag gcagcagcca
     1921 cttcaggaag cacctgcccg gcttcatcga caatgagtaa atcggcaaaa tcatacaaat
     1981 aacttttctc gaatttacgt tgtcctttgt gctcacttat ctgcatattg ccgggcagca
     2041 tatagcatgt catcaccaca catggagtga gtttcattcg acgttgccaa cgggcggtta
     2101 cccctttagc gccttttttc ttcttctctt cctgcagatc atcaatactg gccatatcca
     2161 tcaaccaacg accttcccag tagtgagtcg tcagtaaaaa tgcagggaag cgaatctgtg
     2221 tatcagccag ttcatcggcc tgtgagaagc tcagttcctc gtcgccctca taccctaaat
     2281 caagtgccaa cctctgccac tcctgaaccg cctgctgttc tttaagaaag atttcatggg
     2341 cggagtcaat ctgcttccgg tatgttgttt gctcgcactc agcggaattg agtagctcat
     2401 caatattacg ttcgatagtt tcaggatcag accactgatt tcctgcaatc agcgcgccta
     2461 atttatcttc gagaaacagc tgtatttgga actgtcgctt actacgaacc gccgggagcc
     2521 aggaaaataa tgaatagatc agtgattcac cggcgcgata ttttttccat tccgttttag
     2581 cactcttcag tagagtgact ttttgttctt gtccggaaag taatttattt aaattatcga
     2641 gatattgctc aatgtcgtta gcaataagct cacgcgcagc ccgtacctgg cttaacgttt
     2701 gccatgttgc gttcagtctt atcagttgct cggattttgc tgccaactga ccatgcagga
     2761 gttcaatgac cttttcaggg gatgaacact ctttttcagg aaaagctgcc ttagctttct
     2821 cgagataaaa cagcagagca tcctctacat actcttttga ctcaacctgg ttgaagaaat
     2881 cttcagtttg atattttttg gctgcctcag ctttacgact gcttgaggga aaataagcgc
     2941 cgaagctttt cagctctggc aaccatcgcc cggccattgc accagtgccc tgtgaaaaat
     3001 ctttcccgaa cgcttcgata atgttcgtta cagcctggtt attcgttgaa gtcgcgataa
     3061 taaccggagg ctcagatttt tcgagagccg ctctggccca ctgcgtggcg atgatagaaa
     3121 gcaccagcgt ggtttttccg gttcccggag ggccattaac agcaaggata tcgccatgtc
     3181 ttgcatccag aaaatggctt aaggcatcgc gctgagcctt tgccagcgga aacttatctc
     3241 cggagtgtcc aagcctgtcg ctgaattttg ctcctggggc cagcaaagac tctgcagcat
     3301 gaacctctcg cgaggcgaag cgattgaaga gcggcacatc cttcttgcaa acaagcaggt
     3361 gatcataaag cgaaaggata tggaaactgg caccgccaga ttgcgccgtt ttaacaatat
     3421 aaccgtgctc agcgagttca tattgctcag gatttttaat ccagtcgccg gcaacgttct
     3481 tcagcagcct ttctgaatca tccagatatt gacgccactc ctgctgcaag gctgcatatc
     3541 gtgcttcccg ttcttcatcc gtttcggcag tcttatcaac actgtcatca aagtcgatag
     3601 agaacgaggt atggattgtc ttgtatttgt catactgccc aatctcacca atcgaaaatg
     3661 ctccttttgg caaaggttca agcaggtctc tgggaatgga ggtcgctggc gtcggatata
     3721 aaaaaccctc acggcttagc aaggctggcg tcactatcgg ggtaacaata tcaggcgcac
     3781 ctgcagaacg gtccttacca tgctgcagta accggaaata aacttttggg cgcaagatga
     3841 catcgaccgt ttcgacatcg tctttttctc ccttaaaaaa tttactgaca atcgcttcgt
     3901 caagacgtcc cgccgctatc ccatgccagt gagtgaaatt tttggcgtct ttccgttcaa
     3961 aactgccctt tcctgactca gcatccgcaa gcgagttgcg ccagtatgag gtaaacccta
     4021 aagcattttc atccattacc agtcctgtga ctcaatgaaa tccattgatt tatcgaatat
     4081 aaaaccacca tatggacaac tattgtccat attgaaaaag ttggatgcaa tgttttgttt
     4141 ataattcggt atcaaatgta ctggttgtac aaggttcaga acacaagcac attcaacagt
     4201 acgtcaggac tattgatttc ataacagtga atacattttt taccgtttat aatgactgcc
     4261 tgtgttggac gaggatacca gaacagccga aaacaccggg ggttattacc tgctctcatg
     4321 gaaaccagat attcctggtg ctaatcacag tcataaatag cctgaagaac gtgacgaagg
     4381 gacttaaatg gaagccggag taacttgtca gaacatcaat cttcgtcatc attttcaaag
     4441 aaaaataatt agttaaagca gtccgggcaa tcagcccgga ctgcttacta ttttaatgaa
     4501 ttgttaatct ttgcatattc aaacggactg atacactcct ccctgtttgc atccagaaaa
     4561 tctgcccacc actgtagcat cagtcgacgt tcttccagat gttctgcttt atggatatac
     4621 gcagcacgta caccattacg ctcctgatga ctcatctgac gttccactgc atcgcgggac
     4681 cacaaacctg attcaatcaa tgcactgcat gccatcgccc ggaatccatg gccacagact
     4741 tcccgggttg tgtcatatcc catcaccctc agggctttat ttacagtatt ttcactgatt
     4801 ggtttacgcg ggtcatgtgc tcccgtaaag atcagaccat tttcaccagc ccatgtctga
     4861 agttctgtca gtatggctac agcctgtttg ctgagtggca caagatgtgg tgttcgcatc
     4921 ttggagcccc ggtgtgagaa ttttacccct ggcagaggtt tgcgttcggc tggaatagtc
     4981 cacatggcat tgtcaatatc aatttcagac cagcgggcgt atctgagttc actggaacga
     5041 ataaaaacca gtaaattcag cataacggca agccgggtga caggctgacc tttataactg
     5101 ttaatacgcg acagaagttc aggcaggcgt gaaagattca gagcggggcg atgctggcgt
     5161 tttaccgtgg tcaatgcacc agccatatcc agagccgggt tatatctgat gatgccagac
     5221 tgtacggcgt aacgcattac agcagatatt ctttgctgca gacgactggc gatttcataa
     5281 atttgttttg cttctgcagc acgaacaggg ataagcaggt ccggggtatt gagtgttgtg
     5341 atatcccggt tgccgataaa ggggaaaacg tgtgtctcga ggcttttgag tactttatcg
     5401 ctgtgtgatt gtgcccattg tttattactg gcatgccagc gtcgtgcaat atgttcgaaa
     5461 gagagttgta aatcagggac tactttttta gctcttttct gttcgcaagg gtcaaccccc
     5521 tccgaaatca gttttcgggc ctcatcccgt tttgtacgag cttcggagag cgatacttct
     5581 ggatacacac caagtgccag agtcttctct ttacccagaa tacgataacg gagacgccag
     5641 taacgggaac cattaggatg taccagcagg aacatgccag ccccgtcagt gagtttataa
     5701 gccttgtcag tgggctttgc agcccggatt tttgcgtcag tcagtgccat agttcagcct
     5761 tctgaatgcg ttaaagaggg tacaaattct gtcgaactgg ggtatacccg caattatacc
     5821 cacacaatga ggttgatgtg gggagaattt ggttgagttc ggtagaattg atttggagga
     5881 agaacattta aatcatggtg ttctgaaacg aaaaacggac ctccgtggag gtccgttgat
     5941 atgaat
//
LOCUS       DAZUCC010000249         5926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZUCC010000249 DAZUCC010000000
VERSION     DAZUCC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.250"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            181..762
                     /gene="mdaB"
                     /locus_tag="ACHY09_004478"
     CDS             181..762
                     /gene="mdaB"
                     /locus_tag="ACHY09_004478"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFS3887353.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     ESDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            793..1107
                     /gene="ygiN"
                     /locus_tag="ACHY09_004479"
     CDS             793..1107
                     /gene="ygiN"
                     /locus_tag="ACHY09_004479"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFS3887354.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1152..2039)
                     /locus_tag="ACHY09_004480"
     CDS             complement(1152..2039)
                     /locus_tag="ACHY09_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS3887355.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTMLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2036..2983)
                     /locus_tag="ACHY09_004481"
     CDS             complement(2036..2983)
                     /locus_tag="ACHY09_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS3887356.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAGLTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(2994..4031)
                     /locus_tag="ACHY09_004482"
     CDS             complement(2994..4031)
                     /locus_tag="ACHY09_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS3887357.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4040..5023)
                     /locus_tag="ACHY09_004483"
     CDS             complement(4040..5023)
                     /locus_tag="ACHY09_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS3887358.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILIFDEQTLLAMRTWLAGDLAGQDWVTLGTSAWFSLGGFVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5020..5829)
                     /locus_tag="ACHY09_004484"
     CDS             complement(5020..5829)
                     /locus_tag="ACHY09_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3887359.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1681 a         1614 c         1363 g         1268 t
ORIGIN      
        1 aacttatagg aagttgattt cagtattgtc attttcagaa aatatttatc ccgcatcggt
       61 gaatcagagc aagatcagac ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat
      121 ttcaccgtac cgctctgtga cgtactatag tcgacaaacg tctcaccttg aggttaaaaa
      181 atgagcaaca tcctgattat caacggcgcg aaaaaattcg cccactccaa tggtcaactg
      241 aacgacaccc tgaccgaagt cgcggatggc acactgcgcg acctcgggca tgatgtccgc
      301 atcgttcgcg ccgagagcga ctacgatgtc aaagccgaag tacaaaactt tctctgggct
      361 gatgtggtga tctggcagat gcctggctgg tggatgggcg cgccgtggac agtgaaaaaa
      421 tacattgatg atgtattcac cgaaggtcac gggactctgt atgccagcga tggtcgtacc
      481 cgcaaagatc cgtcgaaaaa atacggttct ggcggtctgg tacaaggtaa aaaatatatg
      541 ctttccctga cctggaacgc accaatggaa gctttcaccg aaaaagatca gttcttccac
      601 ggggttggcg ttgacggtgt gtatctgccg ttccataaag caaaccaatt ccttggtatg
      661 gaaccgctgc cgacatttat cgctaatgac gtgataaaaa tgcctgatgt tccccgctat
      721 actgaagaat atcgcaagca tcttgtggaa atttttggtt aactagagct ctggctttag
      781 aaggagttaa ccatgattac agtaatcgca gaaatccgta ctcgtcctgg tcaacatcac
      841 cgtcaggcag tgttggatca gtttgctaaa atcgttccaa ccgtactgaa agaagaaggt
      901 tgccacggct atgcgccaat ggtggattgc gctgctggcg tgagtttcca gtctatggca
      961 ccggattcta tcgtgatgat tgagcagtgg gaaagcatcg cgcatcttga agcgcatctg
     1021 caaaccccgc acatgaaagc atatagcgaa gccgtaaaag gtgacgtgct ggagatgaat
     1081 attcgtatcc tgcagccggg gctttgacat ttctaaaaag aggccggtgc tgctaccggt
     1141 ctcagcccag attagccatt ctttcttccc gttttcagtc ctcgatggaa atcgttaatt
     1201 cgcttaatgg ttttaaccgc acgctgcggc actgcggaag ctaccgacca gacaatcatt
     1261 tccagacaca acaataccgt accatgtagc ggcgttttcc ctttttcatc cccgcgcgga
     1321 acatgaatca cgatactggc gtctttgcta aaacgtgaat ccagcgcgtt tgtcagcaaa
     1381 ataacaggga tccccagccg cctcgcctca cgaagcattg tcagcccttc ccggtgcgca
     1441 gatttttgcg ccatcatgat cagaacatcg ccacgttgaa gcgcgatcag ttgctcggca
     1501 aggccaatac ccgtacggtt tagtgccgtg gcaggcaaac ctatgcggtt gaatagtcgt
     1561 gcggtatagt ccgccagaat acccgaagcg ccaatgccaa aaatagcgac ttgccgtgcc
     1621 tgcaccaaca acgctacagc ttgcgcaagc gcgtggcggt tttcaggctc tgacaggaca
     1681 ttacaggtat acaaatgccc ttcaagaaca aagtcgattg cggtgttaac atcgctcgtc
     1741 aagttactca ccgtcgtaga catcttttcg ctggaactaa gggctggacc aagccactgt
     1801 tccagagtgc gtttcagatc acgcagcccg gcaaacccca gggcctgtat agccctgata
     1861 actgtggcat cggaagtgtt caacgtggcc gcaatttcca tcgccgtttg ctcgagtacc
     1921 acttcacgat gctcattaat gtagctggcg acaactctta acccaggcgt taactgactc
     1981 gcgcgagtgc gaaaacgctc accaaaaata tcaacccgca ctttatcttt ctggatcatt
     2041 tagcctccgg tagaggtcgc cctgcaattt gtgattgtat ctgcgcagcc atcagcccca
     2101 gagatgcaaa ggagttagaa attgcctctt cacgagaaat cagcacataa cgcccggaac
     2161 gacaggcagt cagaaactga caccatcccg gcatgacttt ttccattgct gccagctcat
     2221 cctgaggttt accaccggtg tcaccgcgcc aggttgcaaa aacaaaatcg gcatccagtt
     2281 caggtaaacg ctccgcgctc acatccattc gcccaccttc aggaatgctc tcaatcagcg
     2341 gcggaaaacg aaaacccgca tcgcgtaata cccgccccag cgaatgatag ctatgcatca
     2401 cattaatttt tccctggttt gcctgaatca ccgataccgt tattttttgg ctgtccagcg
     2461 tcgccttcag cgcattgatt tgtgcctgat agcggcgttc aagtatcgcc agctgcgact
     2521 gcgttcctgt aagtcctgcc agcttgcgat agatttctgg cgcaccccct ttgagatgat
     2581 caatgctcac cgtgggcgca attttttcaa gttgttcaat gggcgtatta cgggtcggtt
     2641 ctgtgatgat caggtcaggc tttgccgcca caatcgcttc aatatcaata tcagcggtac
     2701 caataaaggc gatcgatgaa ttatcaaagt cgacacctgt caataacgcg ccagatcgaa
     2761 tgaaatggct accgtcaggc cgagttcgcc cgtggctggc gacaggaggt acaccgagct
     2821 caatcagagg aatagtgata tcaagatcgt gtaaagagac aattctcttt ggatgagcag
     2881 gaacagtgac tttgcgccct aaatcatcag taaatacctg aacaggttct gttgcacggg
     2941 aaaaaaatga caacagtatc agcagagaaa aaaataaacg catataactc ccgttaaaaa
     3001 ctgttcctgc gctgccagag cagtagcaaa aaaaatggtc caccaatcag tgagataatg
     3061 attccggcag gcagttgcaa aggcaaaaaa gccaaacgac caatgctatc cgccagaata
     3121 actaacaacg cccccgatac cgcgctcccc agcagtaatg tcgtctgccc gccacgtaga
     3181 ataaaacgtg atatatgggg tgctatcaat ccgacaaaac cgatgttacc aacacacgaa
     3241 acgcagacgg ctgtcaggat tattggtgcg gcgacacgta acaacgccag acgagaacta
     3301 ttcaccccta acccggttgc aacctgatga ccaagcagag cgatatccgc cgtcctggcg
     3361 gtgaacagca ataaaagagc ggctggcaac atccaacagg ctgaaatacc gactaacatc
     3421 cagttagctg catgcaggct tcccgataac cacatcaatg cagtttgtac atctcgaaca
     3481 tctgcagtgg tcataaatac accgataccg gcggcgaaaa accaggaaac accaatacca
     3541 atcagaacaa atcgcgggcg ggaaatatcg cgcgcacaaa aaatcacgat taaagccacc
     3601 aacaagccac cagcaagacc agccactgga cgccagaaca ttcccagcat tggaaactgg
     3661 aaaatcagcc acagcaccgc cacgctacat ccttctttga cgccgattaa accaggatcg
     3721 gcaaggccgt tacgcgcaat ggattgcatc gcggcccccg ccatacctaa catggctccg
     3781 catagcaatg ccatgatgac tctcggcaag cgaatatcct gcacaatata acgtgcgtct
     3841 gtgcttacgt tttctggagc aaacaacgct cgtcctactt ccgatgccgg gacagaaaaa
     3901 gaaccatgca tcagaccgaa tatcagtatt ccaaccgcaa acagaaacag cccaccaagc
     3961 aacaccagat ttttagggcg taccagtgtc gaaaacttac caacacgtag tgggcgtagc
     4021 ccgacgttca ttatccgact catttgaaca tcctcgtcgc cataatgaca aaaacaggcg
     4081 caccaactaa cgccgtcatg acaccggtcg ctaactcatg gggcgtgaaa agcgtccgag
     4141 caataatatc tgccagtaat aacaaaagcg cgccgacaca agctgaaaga gggagcagaa
     4201 cgcgcagatc cgctgatacc agacgccgga caatctgcgg tactagcagg ccgacaaaac
     4261 caatggggcc agcaatcgat acggctgcgc cacagagcaa cgcgatcgca agcagggtga
     4321 atgttcgcgt gcggagtacc gagacgccca gcccctgcgc catgcgatcg cccagtgcca
     4381 acatgttaag agagggcgca aggtaaatgg ctaacacaaa tccccccaga gaaaaccagg
     4441 cggacgtacc cagcgtcacc caatcctgcc ccgctaaatc acctgctagc catgtccgca
     4501 tcgccagcag tgtttgttcg tcaaaaatca ggattgcagc cgttattgag gacacgaacg
     4561 ccgatagcgc cacgccgcat aaagtaactt tcattggcgt taaccctgaa cgccctgcgg
     4621 aggaaagcag caatatcaac aggaacaata acgcgccccc cgtagatgcc agcagtgggc
     4681 gtccaaccgg aaaggccagt ccaagcgcac tggcggcaac gacagcaaga gcggctccgg
     4741 catttaagcc taaaatatgt ggctccccca atgggttacg aatgacagcc tgcaacaaag
     4801 caccagcaac accaagagaa gcaccggtaa gcaatgctgc cgctaaacgc ggcaaacgga
     4861 gtcttacaat tacgttgtga tcgaagtttt gcggatcaaa atgaagaaat gcctgcaaca
     4921 ccgtttgtgg tgcaataaat cgggcaccaa tccctggata taccacgcat cccaccagca
     4981 gtaaggcgag gaaaattaca gtaaccagcc ctgtgcgcat catacttttt ctacaccacg
     5041 aaacggcatg aagaacggct ttcccgtcag aggatttatt gatgcgtgaa catcaacgtc
     5101 aaagacagct tttaccagtt caggcgtgca atgctccccc tcattcaaca cacgcaccac
     5161 tttcccctgg cgtaaaaata tcaacgtatc gccataattg acggcaaaat tcagatcatg
     5221 caacaccacg acgactgtac gcccgtgatg acgcgttaaa tcatgtaata actcaagaat
     5281 ttcaacctga taacgtaaat caagccaggt tgtgggttca tccagcagga tataaggcgt
     5341 cttttgcgcc aacaccatcg caatccagca acgctggcgc tgcccaccag agagtttttc
     5401 aacaggtaaa tgtgcaaact cctgcgtacc ggttaatttc agagcctctt ctaccgcctg
     5461 ctcgtcggcg tcgctccatt gacgaatgaa attctgccag ggaaaacgcc cacgcgacac
     5521 cagttcataa acggttaagc cctccggcaa taatggcgac tgcggcaaga tcccaagtct
     5581 acgggataac gctttcgttg gttgctcgtg aatcgctttg ccatcaagca atatcgaacc
     5641 gcccataggt tgcaagatac gtgcaatggt gctgagcaac gtcgactttc cacagccgtt
     5701 tgccccaacc agaacggtca ttttttctgt gggaacggag aaagaaacac catcaacaat
     5761 gatcttcttg tgatacccgg cagaaacatt gtcgaggatc agcccctgcc cttttgcgtt
     5821 atgtgccacg tgtacgccaa cttaatcaaa gacaaacaaa aataaaactc attctccttt
     5881 gagagtttga cgccacaaaa tgtagtagtc aagcagttaa aagcca
//
LOCUS       DAZUCC010000250         5925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZUCC010000250 DAZUCC010000000
VERSION     DAZUCC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.251"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            318..1607
                     /gene="sdaC"
                     /locus_tag="ACHY09_004485"
     CDS             318..1607
                     /gene="sdaC"
                     /locus_tag="ACHY09_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFS3887360.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1665..3032
                     /gene="sdaB"
                     /locus_tag="ACHY09_004486"
     CDS             1665..3032
                     /gene="sdaB"
                     /locus_tag="ACHY09_004486"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFS3887361.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3143..3898
                     /gene="xni"
                     /locus_tag="ACHY09_004487"
     CDS             3143..3898
                     /gene="xni"
                     /locus_tag="ACHY09_004487"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFS3887362.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVVGIGPKSATQLLVEFKSLEGIYENLNAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3953..5101)
                     /gene="fucO"
                     /locus_tag="ACHY09_004488"
     CDS             complement(3953..5101)
                     /gene="fucO"
                     /locus_tag="ACHY09_004488"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS3887363.1"
                     /translation="MANRMILNETAWFGRGAVGSLTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPTLAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5129..5776)
                     /gene="fucA"
                     /locus_tag="ACHY09_004489"
     CDS             complement(5129..5776)
                     /gene="fucA"
                     /locus_tag="ACHY09_004489"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFS3887364.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         1374 a         1588 c         1475 g         1488 t
ORIGIN      
        1 agttcaacct acatatattg cgcgccccgg aagaagtcag atgtcgttta atgggcaaat
       61 attgccctta aattctcttt tacttttgat ttacagagta aagcgttggg ataatctatc
      121 ttccaagtag attattgtat ttgggatcaa gatcactgat agatacataa cttgtgtgta
      181 tctttccgcc ctcaaattat tacggcggta aatgattaag ccatcgccta tagacagatt
      241 tcatttttac ggtcaggcac cttcccgggc tgaactggct aaaagctgaa ttatttgcat
      301 tcctccagga gaaatagatg gaaacgactc aaaccagcac gattgcgtcg aaagactctc
      361 gtagtgcctg gcgcaagaca gacaccatgt ggatgctggg cctttacggc acggcaatcg
      421 gcgcgggcgt gctgttcctg ccaatcaacg ccggtgttgg cggtatgatc ccgctgatca
      481 tcatggctat ccttgcgttc ccgatgacat tttttgctca ccgcggtctg actcgcttcg
      541 tactgtctgg taaaaacccg ggcgaagaca tcaccgaggt tgtagaagaa cactttggta
      601 ttggcgcagg taaactgatt accctgctct acttcttcgc tatctacccg attctgctgg
      661 tttatagcgt ggcaatcacc aatacagttg aaagcttcat gtctcaccag ctgggtatga
      721 caccaccgcc gcgtgcgatt ctgtcgctga tcctgatcgt gggtatgatg accatcgttc
      781 gcttcggtga gcagatgatc gttaaagcga tgagtattct ggtattcccg tttgttggcg
      841 tactgatgct gctggctctg tacctgatcc cgcagtggaa cggcgcagca ctggaaacgc
      901 tgtctctgga cactgcatct gcaaccggaa acggtctttg gatgaccctg tggctggcaa
      961 ttccggtaat ggtgttctcg ttcaaccact ctccgatcat ctcttctttc gccgttgcga
     1021 agcgtgaaga gtacggcgat atggcagaac agaaatgctc gaagatcctg gcattcgcac
     1081 acatcatgat ggtgctgacc gtaatgttct tcgtcttcag ctgtgtactg agcctgactc
     1141 cagcagacct ggctgcggct aaagagcaga acatctcgat tctgtcttac ctggctaacc
     1201 actttaacgc gccgatcatc gcgtggatgg ctccgattat cgcgattatc gctatcacca
     1261 aatccttcct cggccactac ctgggcgcac gtgaaggctt caacggtatg gtgattaaat
     1321 ctctgcgtgg taaaggtaag tctatcgaaa tcaacaagct gaaccgtatc actgcgctgt
     1381 tcatgctggt aacgacctgg attgttgcca ccctgaaccc gagcatcctg ggtatgattg
     1441 aaaccctggg cggcccaatc atcgcgatga tcctgttcct gatgccgatg tacgcaattc
     1501 agaaagtacc ggcaatgcgt aagtacagcg gtcacatcag caacgtattc gttgtcgtga
     1561 tgggtctgat tgcaatctcc gcaatcttct actctctgtt cagctaagtc ctttcgcgcc
     1621 gctttcgggc ggcgcttcct ccgttttaac gcgatgtatt tcctatgatt agcgtattcg
     1681 atattttcaa aatcggcatt ggcccttcca gttctcatac cgttggacca atgaaagcgg
     1741 gtaaacaatt taccgacgat ctgattgccc gtaacctgct taaagacgtg acccgcgtgg
     1801 tggttgacgt gtacggctcg ctctctctga ccggtaaagg ccaccacact gatatcgcca
     1861 ttattatggg cctggcgggt aacctgccgg ataccgtgga tatcgattcc atccctggtt
     1921 ttattcagga tgtgaatact catggtcgcc tgatgctggc aaacggtcag catgaagtgg
     1981 agttcccggt tgatcagtgc atgaacttcc atgccgacaa cctttctctg catgaaaacg
     2041 gtatgcgcat taccgcactg gcgggcgata aagtcgttta cagccagact tactactcta
     2101 tcggcggtgg ctttatcgtt gatgaagaac attttggcca gcaggatagc gcaccggttg
     2161 aagttcctta tccgtacagt tcagcagccg atctgcaaaa acattgtcag gaaaccgggc
     2221 tgtcactctc tggcctgatg atgaaaaacg agctggcgct gcacagcaaa gaagagctgg
     2281 aacagcacct ggcgaacgtc tgggaagtca tgcgtggcgg tattgagcgt ggtatttcta
     2341 ccgaaggcgt gttacctggc aaactgcgcg ttccacgccg tgctgcggca ctacgccgga
     2401 tgctggtcag ccaggataaa accaccactg acccgatggc ggttgttgac tggatcaaca
     2461 tgtttgcact ggcagtgaac gaagagaatg ctgctggcgg tcgcgtggtg actgcgccga
     2521 ctaacggtgc gtgcgggatt atcccggctg tgctggcgta ctacgataag tttatccgcg
     2581 aagtgaacgc taactcactg gctcgttatc tgctggtagc cagcgccatt ggttctcttt
     2641 ataagatgaa cgcgtcgatt tctggtgccg aagtgggttg ccagggtgaa gttggcgtgg
     2701 cgtgctctat ggcggcagct ggtctggcag aactgttagg cgcaagcccg gcgcaggtgt
     2761 gcatcgcggc ggaaatcgcc atggagcata acctcggtct gacgtgtgac ccggttgctg
     2821 gacaggtgca ggtaccgtgc atcgagcgta acgccattgc ggcagtaaaa gcggtgaacg
     2881 ccgcgcgtat ggcgctgcgc cgtaccagcg agccgcgcgt ctgcctcgat aaagttatcg
     2941 agaccatgta cgaaacaggt aaagatatga acgccaagta ccgcgaaacc tctcgcggtg
     3001 gcctggcaat gaagatcgtt gcctgcgatt aatctctccc ggaggcctcg ttttgcgagg
     3061 cctcttcccg ttttctcaac cagccatagc ctgttccggc atcgaatgtt acccttatcg
     3121 cctgatcttt aagggggtta tcgtggctgt tcatttgctt attgtcgatg cactgaatct
     3181 tattcgtcgc attcatgccg ttcagggttc gccctgtgtc gaaacctgcc agcacgcgct
     3241 cgatcagctc attatgcaca gccagccaac ccacgcggtc gccgtttttg atgatgaaaa
     3301 ccgcagtagc ggctggcgtc atcagcgttt accagattac aaagcgggtc gaccgccaat
     3361 gccggaagag ttgcacgacg agatgcctgc attacgcgcc gcctttgagc aacgcggcgt
     3421 cccgtgctgg tccgccagcg gcaacgaagc cgatgactta gccgccacgc tggcggtcaa
     3481 agtgacacag gccgggcatc aggcaacgat tgtttcgaca gataaaggct actgtcagtt
     3541 actttcaccg acattacgta ttcgtgatta cttccagaaa cgttggctgg atgcgccatt
     3601 tatcgataaa gaatttggcg ttcaaccgca gcagttgccc gattactggg gacttgcggg
     3661 gatcagcagt tcaaaggtac cgggtgttgt ggggatcgga ccaaaaagcg ccacgcagct
     3721 gctggtcgag tttaagagtc tggaagggat atatgagaat ctgaatgcgg ttgccgaaaa
     3781 gtggcgcaaa aaattagaaa cccataaaga gatggcgttt ctgtgccgcg atattgcccg
     3841 cttacaaacc gatttgcata tcgacggcaa tttacagcaa ttgcggttgg taaggtaacg
     3901 gcgtgccgga tacggcgcaa acgtcgcatc cggcattatc acatcagcgc atttaccagg
     3961 cggtatggta aagctctaca atatcctcaa gcgttgcttc acgcgggttg ccaccggtac
     4021 aaacatcatc cagtgccgcc tgcgccagtg tcggaatatc ttccttacgt acaccaacat
     4081 cacgtaaatg tggcggaata ccgacatcac ggttgagagc aaacaccgct tcaacagcgg
     4141 cattacgcgc ctcttccagg ctcatacctt ccactttcac gcccataacg cgtgcgatat
     4201 cgcggtactt ctcaccggta aagtcagcgt tataacgcat aacatgcggt aacaggatgg
     4261 cgttcgcaac accgtgtggc gtgttgtaaa acgcgcccag tggatgcgcc ataccatgca
     4321 ccaaccctaa cccaacattc gagaatccca tacccgcaac atactgcccg agcgccattt
     4381 cttctccggc atccttatca ccagcaaccg atcctcgcag cgccccagca atgatttcaa
     4441 tcgctttaat gtgcagcgca tcggttagcg cccacgcgcc acgggtaata tacccctcaa
     4501 tagcatgagt gagcgcatcg acacccgtcg cagctttcag cgctggaggc ataccatcca
     4561 tcatgtcagc gtcaataaac gccacctgcg ggatatcatg cggatcaacg caaacaaact
     4621 tgcgccgttt ttcttcgtca gtgatcacgt agttaatggt cacttctgcc gcagtacctg
     4681 ctgtggtggg gattgccaga atcggtacac tgggtttatt ggtcggggaa agcccttcca
     4741 ggctacgcac atcggcaaac tccgggttgt tgctgataat gccaatcgct ttacaagtat
     4801 cctgtggaga accgccacca atagcgatca ggtaatccgc gccgctattc tggaatacac
     4861 cgagcccttc tttgacgaca gtaattgttg ggttcggcac tacgccgtcg taaatcgccc
     4921 atgccagccc tgcagcatcc atcttatcgg tcacttttgc caccacgccg cattgcacca
     4981 gcgttttatc ggtgacgatc agcgccttct gataaccacg gcgtttcacc tcatcggtta
     5041 aagacccaac agccccccga ccaaaccatg ccgtttcgtt cagaatcatt ctgttagcca
     5101 tcatccttct ccttgttgct ttacgaaatt actcttcaat ccgtaacccg taggttttga
     5161 atttctccag cactacggca atctcttcat cgctcagcac tggcaccggg tccgtaatcg
     5221 ccagggtcgt caggtaaagt tgtgccagca cttcaacttc atgcgccagc cataacgctt
     5281 tctccagatt cgcctcacaa gcgataagcc catgatgttg taacaaagtt gccttacgat
     5341 ttttgagagc cagcgcaaca tgttcagaaa gttcgcgtgt tccaaaggtc gcataaggcg
     5401 cgcaaggaat agaattaccg ccagccgccg caatcatgta gtgaatcgcg gggatcggtc
     5461 ggttaagaat ggaaactgcc gtacaatgaa cggcatgatt gtgaacaacc gcgttggcat
     5521 ccggtctgct ttgataggcc gccatatgga aacgccactc gcttgagggg agttttcctt
     5581 cctcatgttt gccgttgcca tcaataaaga caatatgcga ctccgtcagt ttttcatatg
     5641 gaatgcctgt aggcgtaatc agcatcccat cctgataacg tacactgacg ttacccgctg
     5701 tcccctggtt cagtcccagg cgggtcattt ccaggcaagt gtcaataatc tgacgagcaa
     5761 gtttatttcg ttccattagc tacctctctc tgattcaaaa cagggcaata atgttgttcc
     5821 tttcacacta ttgaattagc cgtttaatta cccaccatct tcttcctgat taaccagaaa
     5881 gaaattcaca agcttatatt ttgtgacctg gttcaactaa tcaca
//
LOCUS       DAZUCC010000251         5872 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZUCC010000251 DAZUCC010000000
VERSION     DAZUCC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.252"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..306
                     /locus_tag="ACHY09_004490"
                     /pseudo
     CDS             1..306
                     /locus_tag="ACHY09_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(505..1629)
                     /gene="yhhJ"
                     /locus_tag="ACHY09_004491"
     CDS             complement(505..1629)
                     /gene="yhhJ"
                     /locus_tag="ACHY09_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3887365.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1629..4364)
                     /gene="rbbA"
                     /locus_tag="ACHY09_004492"
     CDS             complement(1629..4364)
                     /gene="rbbA"
                     /locus_tag="ACHY09_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFS3887366.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGSVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYHPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     ACLQEAAGQSNEAEAPPVIHDTTHAARQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMVPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4361..5428)
                     /gene="yhiI"
                     /locus_tag="ACHY09_004493"
     CDS             complement(4361..5428)
                     /gene="yhiI"
                     /locus_tag="ACHY09_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS3887367.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(5491..5604)
                     /gene="yhiY"
                     /locus_tag="ACHY09_004494"
     CDS             complement(5491..5604)
                     /gene="yhiY"
                     /locus_tag="ACHY09_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFS3887368.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
BASE COUNT         1364 a         1668 c         1519 g         1321 t
ORIGIN      
        1 attctactgt tgactatttg tgccgttatt tctggtgcag aaggttggga agatatagag
       61 gattttgggg aaactcatct cgattttttg aagcaatatg gtgattttga aaatggtatt
      121 cctgttcacg ataccattgc cagagttgta tcctgtatca gtcctgaaaa atttcacgag
      181 tgctttatta actggatgcg tgactgccat tcttcagatg ataaagacgt cattgtaatt
      241 gatggaaaaa cgctccggca ctcttatgac aagagtcgcc gcaggggagc gattcatgtc
      301 atttctcgcg tcagtccttg cggggagcgg gctttcgtaa tcttgccctg aataccggac
      361 tgagtataaa aaagccggtt aactgaaact gtccaggttt tggtggtcag ttcataaacg
      421 ccttatccgg cctaaaaaaa actaaaattc aataaattgc accaatgcgt agaccctata
      481 agcttacgca tcgggcaact gtaattacgc cattgtccca attgtcttcc tgaatctcag
      541 cagtgcgatg gtgaaaaacg cgccgccaat tgccatcagt gtcaggaact gcggccagac
      601 gatttcgaat ccggcaccac ggtagagaat ggcctgcgcg aggctaacaa agtgtgtcgt
      661 cggcatggtc agcataatgt cctgcaccat ctgcggcata ctttcgcgcg gcgtggaacc
      721 accggaaagc atttgcagcg gcagcagcac cagaatcacc agcagcccca gttgcggcat
      781 tgaacgcgct atcgtcccca taaaaatacc gattgacgtg gtggcaaaca gactgagcgc
      841 cacgcccagc ataaacagcg ggatcgagcc ttctatcggt acgcccagca cgccttttac
      901 catcagcacc agcgataatc ccgacaccac cagcaccacc agccccatcg accagacctt
      961 tgccatcatg atctcaaacg gcgttatcgg catcaccagt aagtgttcca ccgtgccgtg
     1021 ttcacgctcg cggatcagcg ccgatccggt caatacaatc gccagcatgg taatgttgtt
     1081 gatgatcgcc atcaccccgc caaaccacgc gggatcaagg ttcgggttaa agcgcatccg
     1141 tgtttccagc gataccaacg gttcgctgtt atcacggtag cgcgcgacaa agctgttcac
     1201 ttcaccgttg ataatattct ggatataccc attgccggta aatgcctggc tcatgcgcgt
     1261 ggcatcgacg ttcacctgaa tatccggctg gcgtccggcg aggacatcac gctgaaaatt
     1321 aggcggaata tttatcgcga aggtataacg tccggcgtcc agcccggcat ccatctcatc
     1381 ggcggtgatc atctccggtg gcaaaaacca cgggcgatag aagctgttaa cgatccggtt
     1441 cgataactgc gattgatcca tatcggcaat ggcgatcggc gcgaggttca acgatcctgg
     1501 cgtaacggtc gctgacgaat acaccgacac cgtaaacgag aagacaatca gcgtcagcat
     1561 cgctttatca ccgagcagac tgcgcaactc tttgataccc agattaaaaa tattacgtaa
     1621 atggcgcatc atccctcctg ttttttcagc agcaggatgc ttaagcccat caccagcggt
     1681 atggctatca gcaacgggat aaaaagttgc cacaaatcag tcagatccag cgctttcgag
     1741 aacgttccgc gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc gatccaacgt
     1801 cctggacctt ccagcgaagc taccggatcg atcatcccgg aaaactgtgt cgccgggatc
     1861 aacgtgataa ttgccgtacc aaaaatggcg gcaatctggc ttttcataaa ggtggagatc
     1921 agcagcccca ttccggtggc gatgatgata tacagcagcg ccgccagggt gagagtcagg
     1981 aaactgcctt tatgcggtac gccaaataca aataccgaca gggcgcagag caggaaaaag
     2041 ttcagcatcc ccagcgcgat gtatggcagc tgtttgccaa gcaaaaattc gctacgggtg
     2101 gtgggggtca cgtaaaggtt gataatcgac cctagctctt tttcccgcac cacgctaagg
     2161 gcgcttagca ttgacgggac catcatcagc agaagcggga tcaccgccgg aacaatcgcc
     2221 ggcaggcttt ttacgtccgg gttatagcga tagcgcgtct caatattcat cagcccgctt
     2281 tggctggcgg gtgtggactg tcggctcgcc acatcctgta gccagctctg gtgcatggcc
     2341 tgcacgtagc cttttaccgt ttcggcgcgg ctcggcatcg caccgtcgat ccagacaccg
     2401 agttccacgg gcgtaccacg cgcgatatcg cgcccgaaat taggagggat ctcaattgcc
     2461 accgtgatat cgcccgcacg catccgacga tcaagctcgt cataactggt gagcggcggc
     2521 tgttcgataa agtaacggga accggagaga ttgagcgtcc acgcctggct actgacggtt
     2581 tgatcgcggt caagcaccgc aaagcgcagg ttttccacat ccatactgat gccgtaaccc
     2641 attatcagca tcaggatcac cgttcccatc agcgccagcg tcgaacgtac cggatcgcgt
     2701 cgcagttcca gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct aaatccctga
     2761 cgcgccgcgt gggtggtgtc gtgtatcacg ggcggcgctt cggcttcgtt gctctgccct
     2821 gccgcttcct gcaaacaggc gataaatgcc tcttccagac ttgcggctcc gcgtttctca
     2881 accagttcct gcggtgtccc gctggcgagc acttttccgg cgtgcatcag tgagatacgg
     2941 tcgcaacgtt ccgcttcgtt cataaagtgg gtggagatga agatagtcac tttgtcctgg
     3001 cgcgagagat cgaccatcaa ctgccagaac atatccctcg ccaccggatc gacgccagaa
     3061 gtaggctcat cgaggattaa catctccggg cgatgaatca ccgccaccgc cagcgaaagc
     3121 cgctggcgaa tgccgagcgg caatgactcc ggcagaacat cttcaacgtc gttgagctta
     3181 aaacgctcgc tcatttcagc cactcgcgcg ggaatttccg cttccgggat gtgaaacaaa
     3241 cgggcatgta actcaaggtt ttgccgcacg gtgagttcgt tatagagcga aaacgcctgc
     3301 gacatatagc ctacccgacg gcgggtatcg atatcttttg gatcaaccgg ttgcccaaac
     3361 agccacgcct caccttcgct ggcgggcagc agtccggtga gcattttcat ggtggtggat
     3421 ttaccgcagc cgttcgaacc gaggaatcca aaaatctccc cgcgtggaat gcggaaattg
     3481 acgtgatcaa cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc ttcgatggca
     3541 atctctgcgt tttcagggtg atacggtggg atcactaccg cctgatgcgc ctggcgttgc
     3601 gcttgcggta acagatttat aaatgcttct tccagcgtgg cgctttgcgt ttgctgccgc
     3661 agctcttcgg cgctgccggt tgccagcact tctccggcat tcatcgctac cagccagtcg
     3721 aagcgttcgg cctcttccat gtaggcggtg gcgaccagca cgctcatatt gctctgccgc
     3781 tggcgaatgc tgtcgatcaa atcccagaac tgggcgcggg agagcgggtc aacccccgtt
     3841 gttggctcat caagaatcaa cagttccggg tcgtggatta acgcacagca cagcccaagt
     3901 ttttgcttca tcccgccgga gagtttccct gccggacgat cacgaaacgg tgctaacccg
     3961 gtgctggtca gcagctcatt gattcgcact tcccgctccg ctttgtcgtg accgaacagg
     4021 cgagcgaaaa aatcgacgtt ttcatacacc gacaaggtgt ggtagaggtt tttgcccagc
     4081 ccctgcggca tccaggcgat gcgcgggcaa acgtcgcggc gatgcttcgg gtcgcgcata
     4141 tcgccgccca gcaccatcac gctgccctgc tcaatgacgc gggcaccgga aatcaacgac
     4201 aacaagctcg atttaccgac accatccggt ccaatcagcc cgaccatgca acgggccgga
     4261 atatcgagtg tgatattgtt cagcgcaacg gtttttccat aatgctggct cacgcccgcc
     4321 agttgcgcga caggcgggac gggaaccagt tccagatgcg tcattgcggc aacctcacca
     4381 ccaggtcgtc aggccacgga agttcttcat tcacccgcac ccacgctacg cccggcaatc
     4441 cggttttgac atattccaga tgctgctgga gtaattccgg tgggatacgc gctttgacac
     4501 ggaacatcaa tttcagccgc tcatcgctgg tttcgacggt ttttggcgtg aactgggcga
     4561 cgctggcgac aaaactgatg gtcgcgggaa tacgcagatc tggtgcagca tcaagaatca
     4621 aacgtgcttc accgcccagt ttcagcgtgc ccgcctgttc ggttggcaga aagaaggtca
     4681 tatagacgtc gctgagatcg accatattca gcacccgacc gcctgccgcc agcacttcgc
     4741 ctggctcggc aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt tcgctgtcat
     4801 cgatatctgc ggcaatgcgc cgttcagtgg cttgtgccgc ttcgacgcga gtttgcgcct
     4861 gaatgatatt ggtgcgtgcc gcctctatag cggctttaga agcagaaacc tgagcttttg
     4921 ccgattccag cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc aactgttgcg
     4981 cagaaattgc cccccgttgt gccagtgagc gggaacgcgt atgacgtttt gctacggagt
     5041 ccagttctgc ctggcgttga ttaaccagcg actgtgcggc acgagtttcg ctttggcgtt
     5101 gctccagcaa agcctgcgcg gcggcaacgg cactttgtgc ttctttgatt tgcgcgatgg
     5161 cttccagtcg ctgttcctgc aacacgcgag tatccatctt cgccagcact tcaccttctc
     5221 gaacaaactg gccttctttc accagaatgg tgtcgatacg cccggcaatt ttgctggcga
     5281 tatccacttc cgtcgcttca atgcgcccat tgctgacagc aaagccttcc ggcacacccg
     5341 ccgggcgcaa cagccaccac gccacgacgg cagccaccgc cagtgccccg acaacccacc
     5401 acgccagatg gcgcttactc ttatccataa tcgatccgcc ataatccctg taagcaatcg
     5461 acgcgtgtta caactgccac cgaacgatgt tcagcggatt cttccatccg ggataagaag
     5521 gaaatggcaa atacgaccag cgcacagagc attgagcgcc aatggggaag gcttagtgaa
     5581 aacgggtagt aatgttgtca tcatcagcat tcctggccgt aaatgaaaca cgcatactca
     5641 taagtgaaat caattaaata ctaacagtta tattcatgtt gtctatttga ttgccatcaa
     5701 cttgtgatac atcgcgatgt tcttccgttc gctggtgttt tttccaaaaa tattctgtac
     5761 aggttcacag attctgttgc acaggtttcc gtaacaggga aacctgatga ctatcacata
     5821 gcgttattat cgcctgatgt gcgaataaat accgaatata aaataattta tt
//
LOCUS       DAZUCC010000252         5827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZUCC010000252 DAZUCC010000000
VERSION     DAZUCC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.253"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHY09_004495"
     CDS             complement(<1..593)
                     /locus_tag="ACHY09_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS3887369.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHY09_004496"
     CDS             complement(664..2778)
                     /gene="fusA"
                     /locus_tag="ACHY09_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS3887370.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEEEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHY09_004497"
     CDS             complement(2875..3345)
                     /gene="rpsG"
                     /locus_tag="ACHY09_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS3887371.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHY09_004498"
     CDS             complement(3442..3816)
                     /gene="rpsL"
                     /locus_tag="ACHY09_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS3887372.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHY09_004499"
     CDS             complement(3942..4229)
                     /gene="tusB"
                     /locus_tag="ACHY09_004499"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFS3887373.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLAGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHY09_004500"
     CDS             complement(4237..4596)
                     /gene="tusC"
                     /locus_tag="ACHY09_004500"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFS3887374.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHY09_004501"
     CDS             complement(4596..4982)
                     /gene="tusD"
                     /locus_tag="ACHY09_004501"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFS3887375.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRCWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4982..5704)
                     /locus_tag="ACHY09_004502"
     CDS             complement(4982..5704)
                     /locus_tag="ACHY09_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3887376.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
BASE COUNT         1434 a         1515 c         1422 g         1456 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtca
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgacccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      481 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      541 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      601 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      661 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      721 cttcaggaat tccatggtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      781 atatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      841 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      901 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      961 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
     1021 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
     1081 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
     1141 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
     1201 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1261 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1321 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1381 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1441 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1501 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1561 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1621 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1681 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1741 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1801 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1861 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1921 caggtaatcg attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1981 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttaatctc
     2041 ttcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     2101 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     2161 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     2221 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2281 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2341 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2401 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2461 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2521 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2581 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2641 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2701 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2761 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2821 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2881 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2941 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     3001 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     3061 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     3121 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     3181 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3241 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3301 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3361 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3421 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3481 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3541 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3601 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3661 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3721 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3781 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3841 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3901 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3961 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgagaatgat atcgttcgaa
     4021 atttgaccag ccaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     4081 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac accatcttgc
     4141 aataatagca gttcgtcgcc ctcacttagc aggcgcagca gcgcagcaaa atccgtcagc
     4201 cagggtgaac gatgtaatgt gtgcagcata aacgcctcaa aacctcaaaa taacatcgta
     4261 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4321 tgtctgcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4381 gtcgtacaga cccaacaatt taaaagtggc gatgtaatca cgcgccagca ctgcatcggg
     4441 cttttgtcct ggcagcagct gaaaaacgcc atcagcaata aagaagacgg ccagatcgtc
     4501 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4561 aggcacagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4621 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccgg ctgaaggttt
     4681 gacgaagcca gccccagtct tccggcctcc gtttcatcga cgataccgcg gcgtaacgct
     4741 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagcagcgc
     4801 acaaggtcaa attcatcgct tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4861 cgataaaaaa agacgctgct taactcatgg ccttctgcaa tcagcgcctg cgcaaactga
     4921 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4981 attacttatc ttgcccctgg aaatcaccgc tcttgaactg gcggatgtag agatagacag
     5041 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcatcttta atatcgaaga
     5101 tccctttctc gtagagattc agcacgatct gacgattttt ggcgttatta gaaacattgc
     5161 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     5221 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaggtgctca
     5281 taatctgcga gaagggaaca tcaagattca tattgatgca cagcaaacca attacacgct
     5341 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5401 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5461 ccaggtcggt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5521 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5581 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5641 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5701 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5761 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5821 cggcctg
//
LOCUS       DAZUCC010000253         5824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZUCC010000253 DAZUCC010000000
VERSION     DAZUCC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.254"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            70..1125
                     /gene="phoE"
                     /locus_tag="ACHY09_004503"
     CDS             70..1125
                     /gene="phoE"
                     /locus_tag="ACHY09_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFS3887377.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLESDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1164..1565)
                     /gene="crl"
                     /locus_tag="ACHY09_004504"
     CDS             complement(1164..1565)
                     /gene="crl"
                     /locus_tag="ACHY09_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFS3887378.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(1623..2867)
                     /gene="frsA"
                     /locus_tag="ACHY09_004505"
     CDS             complement(1623..2867)
                     /gene="frsA"
                     /locus_tag="ACHY09_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFS3887379.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2959..3417)
                     /gene="gpt"
                     /locus_tag="ACHY09_004506"
     CDS             complement(2959..3417)
                     /gene="gpt"
                     /locus_tag="ACHY09_004506"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS3887380.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            3678..5135
                     /gene="pepD"
                     /locus_tag="ACHY09_004507"
     CDS             3678..5135
                     /gene="pepD"
                     /locus_tag="ACHY09_004507"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFS3887381.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5192..5544)
                     /gene="prfH"
                     /locus_tag="ACHY09_004508"
                     /pseudo
     CDS             complement(5192..5544)
                     /gene="prfH"
                     /locus_tag="ACHY09_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5540..5746)
                     /locus_tag="ACHY09_004509"
                     /pseudo
     CDS             complement(5540..5746)
                     /locus_tag="ACHY09_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
BASE COUNT         1505 a         1469 c         1428 g         1422 t
ORIGIN      
        1 aaaactttcc tgttttcaac gggactctcc cgctgaatat tcgcgcgtta attaaaatca
       61 ggaatgaaaa tgaaaaagag cactctggca ttagtggtga tgggcattgt ggcatctgta
      121 tccgtacagg ccgcagaaat atataacaaa gacggtaata aactggatgt ctatggcaaa
      181 gttaaagcca tgcattatat gagtgataat gacagtaaag atggcgacca gagttatatc
      241 cgttttggtt ttaaaggcga aacacaaatt aacgatcaat tgaccggtta tggtcgctgg
      301 gaagcagagt ttgccggtaa taaagcagag agtgatactg cacagcaaaa aacacgtctc
      361 gcttttgccg ggttgaaata taaagatttg ggttctttcg attatggtcg taacctgggc
      421 gcgttgtatg acgtggaagc ctggaccgat atgttcccgg aatttggtgg cgactcctcg
      481 gcgcagaccg acaactttat gaccaaacgc gccagcggtc tggcgacgta tcggaacacc
      541 gacttcttcg gcgttatcga tggcctgaac ttaaccctgc aatatcaagg gaaaaacgaa
      601 aaccgcgacg ttaaaaagca aaacggcgat ggcttcggca cgtcattgac atatgacttt
      661 ggtggcagcg atttcgccat cagtggagcc tataccaact ctgatcgcac caacgagcag
      721 aacctgcaaa gccgtggcac aggcaagcgt gcagaagcat gggcaacagg tctgaaatac
      781 gatgccaata atatttatct ggcaactttc tattctgaaa cacgcaaaat gacgccaata
      841 actggcggct ttgccaataa gacgcagaac tttgaagcgg tcgctcaata ccagtttgac
      901 tttggtctgc gtccatcgct gggttatgtc ttatcgaaag ggaaagatat tgagggcatc
      961 ggtgatgaag atctggtcaa ttatgtcgat gttggtgcta cgtattattt caacaaaaat
     1021 atgtcagcgt ttgttgatta taaaatcaac caactggaaa gcgataacaa attaaatatt
     1081 aataatgatg atattgtcgc ggttgggatg acgtatcagt tttaatgaat attgccggat
     1141 gtgatgcatc cggcacattt cattcacgcc gttaacttca ccggttcatc acgaaaatca
     1201 tctgccggtt ccagcttcag attcagcgtc gccagcagct cacgcagctt ctcgtgaaat
     1261 tcacgcaggg tgtgctccag tcgttcaacc acttcggcat cttttatctg aacactcgtc
     1321 cagtcgcctg ctttatcgaa cagaccaaac tggtaactgt aggtaaaacg ggattcctgc
     1381 gcttccagtt ccatccacca gccccagaac tcacgcactt ccggtgccgg tttcacgttg
     1441 acgcatacag ccagacaatc gaaaaagaat cgattatctt cgcacttacc ttcacgaata
     1501 tacgggccta gtgcggtaaa ttttttgatc aatctgctct tcgggtgtcc actcggtaac
     1561 gtcattgcaa tctcctgttg tgatgcaact gttttaccaa attggtaaaa tttagcaaat
     1621 ttttaacaca aacgtttttc gatccagtct gtgatttcct gaaggccttt gtcaaaattc
     1681 cgatacaccg ggttaaatgg gatctctaat aatttaccgt cagcagatga cgacgtgatt
     1741 aagcgtgagt cctcttccgg gctgaacgga tcgttcttcc agtagcctga taacattggc
     1801 gttgggcagc gacgtcccag caatccttgc acttttaatg aatagcgatt caactccact
     1861 cgcaacgctt catcggaagc atcatgcatc cctaaacgac tggccagaac gtcaagatac
     1921 atttccggca cctgttgctg gcacttaaaa tcactcaaca gggtatgaac taccggacca
     1981 agacaggcaa ccactttcag acgcggcgat tcaaggtatg ccagacgcac ggcaacgtta
     2041 gcgccgaaac ggaaaccaaa ggccgcgacg cgagtgtgat ccacccacgg tacgttaggc
     2101 agcgccttta aaacgtgctg atgcaacagg ctggagtcct gggtgagctt ccattttgaa
     2161 gaaaagccca ccgacggcat atcaatagtc agcatagcaa tgccgcgcgg cgcaaaataa
     2221 cgttcataca ggctgtaata gtccgtctgc atcgcatcca gaccaccaca cattaatact
     2281 gtcgggaacg ggccatcgcc tttcggcata tgcaaaaagc cggtgatggg cgcaccgccg
     2341 ggtacggtaa actccatctg ccgcatcgtg cccggcagac gctgagcggc ctcttcatag
     2401 gcgcggtttg acaaagcctg cgcttgctcg gccaggtcat ctcctttcag atgaggatag
     2461 gcagcaatgt tgtacaacgt agccgcatgc agccagtatc gaccgctgag ttgcggatct
     2521 tcttccgcac aggctttttg ttgccacacc atcgcctggg tagcccactc ataaatccag
     2581 ttgccgccgc ggtagccaat caccgtatcg tataaatcat cgtcggtacg ttcggcatcg
     2641 ctcatcacaa tacgcgcctg gacgtcgagg atttcacggg ggtcaatgcc gcgccagatc
     2701 cacatcagac ggttaatcat gcgataccag tgagggatgg ttttaccatc aagggccgac
     2761 tgcacaggca gttgtgcgcc gtgactaaag cggcggacta gcgtcgaggt ttcaggatgt
     2821 ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga tgcagcctcc
     2881 agaatactta ctggaaacta ttgtaacccg cctgaagtta aaaagaacaa cgcccggcag
     2941 tgccaggcgt tgaaaagatt agcgaccgga gattggcggc acgaatacga cgcccatatc
     3001 ccacggctgt tcgatccagg tattttgcgg gatatcaaca acataatcat caaccagcgg
     3061 acgaccagcc ggttttgcga agatggtgac aaagtgcgct tttggataca tttcacgaat
     3121 cgcaaccgca gtaccaccgg tatctaccag gtcatcaata acgatgaagc cttcgccatc
     3181 gccttctgcg cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt agctggaaat
     3241 acaaacggta tcgacatgac gaatacccag ttcacgcgcc agtaacgcac ccggtaccag
     3301 accgccacgg cttacggcaa taatgccttt ccattgttca gaaggcatca gtcggcttgc
     3361 gagtttacgt gcatggatct gcaacatgtc ccaggtgacg atgtattttt cgctcatgtg
     3421 aagtgtccca gcctgtttat ctacggctta aaaagtgttc gaggggaaaa taggttgcgc
     3481 gagattatag agatctggcg cactaaaaac cagtatttca catgagtccg cgtcttttta
     3541 cgcactgcct ctccctgacg cgggataaag tggtattctc aaacatatct cgcaagcctg
     3601 tcttgtgttg acaacatttt ctgctaaccc tgtgacctgc aatactgttt tgcgggtgat
     3661 cgacaaggag acttaacgtg tctgaactgt ctcaattatc tccacagccg ctgtgggata
     3721 tttttgccaa aatctgttct attcctcacc cgtcctatca tgaagagcaa ctcgctgaat
     3781 acattgttgg ttgggcaaaa gagaaaggtt tccatgtcga acgcgatcag gtaggtaata
     3841 tcctgattcg taaacctgct accgcaggta tggaaaatcg taaaccggtc gtcttgcagg
     3901 cccacctcga tatggtgccg caaaaaaata acgacaccgt gcatgacttc accaaagatc
     3961 ctatccagcc ttatattgat ggcgaatggg ttaaagcacg cggcaccacg ctgggtgcgg
     4021 ataacggcat tggtatggcc tctgcactgg cggttctggc tgacgaaaac gtggttcacg
     4081 gcccgctgga agtgctgctg accatgaccg aagaagccgg tatggacggt gcttttggct
     4141 tacagagcaa ctggttgcag gctgatattc tgattaacac cgactccgaa gaagaaggtg
     4201 aaatctacat gggttgtgcg ggaggtatcg acttcacctc caacctgcat ttagatcgtg
     4261 aagcggttcc agctggcttt gaaaccttca agttaacctt aaaaggcctg aaaggcggtc
     4321 actccggcgg tgaaatccac gttggcctgg gtaatgccaa caaactgctg gtgcgcttcc
     4381 tggcgggtca tgcagaagaa ctggacctgc gccttatcga tttcaacggc ggcacactgc
     4441 gtaacgccat cccgcgtgaa gcctttgcga ccattgctgt cgcagctgat aaagtcgacg
     4501 ctttgaaatc tctggtgaat acctatcagg agatcctgaa aaacgagctg gcagagaaag
     4561 agaaaaatct ggccttgttg ctggactctg ttgcgaacga taaagctgcc ctgattgcga
     4621 aatctcgcga tacctttatt cgtctgctga acgccacccc gaacggtgtg atccgcaact
     4681 ccgacgtggc aaaaggtgtg gtcgaaacct ccctgaacgt cggtgtggtg accatgactg
     4741 acaataacgt agaaattcac tgcctgatcc gttctctgat cgacagcggt aaagactacg
     4801 tggtgagcat gctggattcg ctgggtaaac tggctggcgc gaaaaccgaa gcgaaaggcg
     4861 catatcctgg ctggcagccg gacgctaatt ctccggtgat gcatctggta cgtgaaacct
     4921 atcagcgcct gttcaacaag acgccgaaca tccagattat ccacgcgggc ctggaatgtg
     4981 gtctgttcaa aaaaccgtat ccggaaatgg acatggtttc tatcgggcca accatcaccg
     5041 gtccacactc tccggatgag caagttcaca tcgaaagcgt aggtcattac tggacactac
     5101 tgactgaact gctgaaagaa attccggcga agtaattatt tgatttgctg ccggatggcg
     5161 tttaatcgcc ttccggcagt ttcatccttc attatccttc gataaaagcc atccctgtga
     5221 acgttcgtcg cgggttgcca cgttcaatct ggtgatggaa cattcgccgc tgcgatttca
     5281 gcgccgcgct attttcctgt tgctgttgtt ccagcttcca ggcaatcagc aatcgcgcca
     5341 gccgcttgtt agcatgccca gaaaccagtt tttgcgccca tgatgaggcc gatacggact
     5401 cggacaaata cactgaatag tgccgcacca actttcgctt aatgcccatg cattatcgcc
     5461 atccagagaa atcagcgccg aacgcagcgt gtcagagtag cggcccgttt ctgtttccag
     5521 caccgttacc gcgacgtctt gccgggtaaa aacgaccaac agagaagaac gctgcgccaa
     5581 tcgggtagcc gcgtccggga tgtaaatctg gcatccccac aacgcgctgc atgttgggta
     5641 aatttgccgt ggtgtgtaat tgttggatcg ctaaactctc gatccacagg tcatcagatg
     5701 cgatggtaaa taccgcatcg gataagggac gaatatattt gcccatgtat cattccattt
     5761 atactcgtca tacttcaagt tgcatgtact gcgaccgcgt tcgttcaccc cagtcactta
     5821 cttt
//
LOCUS       DAZUCC010000254         5812 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZUCC010000254 DAZUCC010000000
VERSION     DAZUCC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.255"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            108..719
                     /gene="ruvA"
                     /locus_tag="ACHY09_004510"
     CDS             108..719
                     /gene="ruvA"
                     /locus_tag="ACHY09_004510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS3887382.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            728..1738
                     /gene="ruvB"
                     /locus_tag="ACHY09_004511"
     CDS             728..1738
                     /gene="ruvB"
                     /locus_tag="ACHY09_004511"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS3887383.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(1888..2673)
                     /gene="znuB"
                     /locus_tag="ACHY09_004512"
     CDS             complement(1888..2673)
                     /gene="znuB"
                     /locus_tag="ACHY09_004512"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFS3887384.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAIMFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2670..3425)
                     /gene="znuC"
                     /locus_tag="ACHY09_004513"
     CDS             complement(2670..3425)
                     /gene="znuC"
                     /locus_tag="ACHY09_004513"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFS3887385.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRHELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3504..4436
                     /gene="znuA"
                     /locus_tag="ACHY09_004514"
     CDS             3504..4436
                     /gene="znuA"
                     /locus_tag="ACHY09_004514"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFS3887386.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            4452..5774
                     /gene="mepM"
                     /locus_tag="ACHY09_004515"
     CDS             4452..5774
                     /gene="mepM"
                     /locus_tag="ACHY09_004515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFS3887387.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
BASE COUNT         1521 a         1472 c         1501 g         1318 t
ORIGIN      
        1 cagatcatcg ggacacctcg caagttttct tcatccttcg ctggatatct atccagcatt
       61 tttttatcat acagcattat ctttgattca ttacgcagga gcgtcatgtg ataggcagac
      121 tcagaggcat catcattgaa aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag
      181 gctatgaagt gcatatgccg atgacctgtt tttatgaact ccctgaagcg ggtcaggaag
      241 cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca actgctgtac ggttttaaca
      301 ataaacaaga gcgcacgttg ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt
      361 tggcgctggc gatcctctcc ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg
      421 aagaagtggg ggcactggtg aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga
      481 ttgtcgaaat gaaagaccga tttaaaggtt tgcatggcga tctctttacg ccagccgccg
      541 acctggtact cacgtcacct gccagcccgg cgaccgacga tgctgaacag gaagcggttg
      601 ctgcgctggt ggcgctgggc tataaaccac aagaagcaag ccgtatggtg agcaaaatcg
      661 ctcgccctga cgccagcagt gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag
      721 gtaaaggatg attgaagcag accgtctgat ttctgccggt accactttgc cggaagatgt
      781 ggcagatcgc gccattcgcc ctaaattact ggaagagtat gttggtcaac cgcaggttcg
      841 ttcacagatg gagattttca tcaaagcagc gaaactgcgc ggcgatgccc tcgatcatct
      901 gttgattttt ggtcctccgg ggttgggtaa aactacgctt gccaatattg tcgccaatga
      961 aatgggcgtt aatttacgca cgacttctgg tccggtgctg gaaaaggcgg gcgatctggc
     1021 agcaatgctc actaaccttg aaccacacga tgtactgttt attgatgaga tccaccgtct
     1081 ttcaccagtg gtggaagagg tgttgtatcc ggcaatggaa gactaccaac tggatatcat
     1141 gattggtgaa ggtccggcgg cacgctccat taaaattgat ttgccgccgt ttacactgat
     1201 tggcgcaacc acgcgcgcag gttcgctgac atcaccgttg cgcgatcgtt ttggtattgt
     1261 gcagcgtctg gagttttatc aggtgccgga tctgcaatat atcgtcagtc gcagcgcacg
     1321 ctttatgggg cttgagatga gtgatgacgg cgcgctggaa gttgctcgtc gcgctcgcgg
     1381 tacgccgcgc attgctaacc gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca
     1441 cgatggcacc atctcggctg atatcgctgc tcaggcgctg gatatgctga atgtcgatgc
     1501 cgaaggtttc gattatatgg accgcaaatt gttgctggcg gtaatcgata agttctttgg
     1561 tgggccggta ggtctggata acctggcggc tgccattggc gaagaacgtg aaaccattga
     1621 ggatgtgctg gaaccttatt tgattcagca aggctttttg cagcgtacac cgcgcgggcg
     1681 tatggcgaca gtgcgggcgt ggaaccactt cggcatcacg ccgccagaaa tgccgtaagt
     1741 cggatcggtc gctcgcggcg catccgacac tggcagtgtt gcctgatgcg ccgctattcg
     1801 cgtcttatca ggcatatacc agagatagcg aaccataggt cggataaggc gctcgcgccg
     1861 catccgacaa atgtgttcag cgatagatta gctggcctgc tttttcatca tactgataat
     1921 aaataacagt gccgcacata gcaccaccga cgggcctgca ggtgtatcgt aaaatgcgga
     1981 aaaggttaaa ccgccagtca ctgccaccat ccccaccaaa acagcgacac cagccatctg
     2041 ttccggcgtg cgggcaaagc gacgtgcagt agcagcagga ataatcagca gtgaagtaat
     2101 aatcaacgcg ccgacaaatt tcatcgctac accaatcgtc aatgccgtca ccagcatcaa
     2161 caacaatttc acgcgctgta atttcacacc atcaacaaac gccagatccg ggctaatcgt
     2221 catcgacagc aaattgcgcc attgccagaa cataatagcc accacgatga ccacgccaat
     2281 cgcaatagag atgagatctt ctggcgtcac tgccagtaaa tcaccgaaca ggtaagccat
     2341 caaatcaaca cgaatattag acatcagact aacgaccacc aggcccagcg acagggcact
     2401 gtgcgccata atccctaata acgtgtcgat cgccagctgt ggacgcttct ccagccatac
     2461 cagaccgccc gccagcagca gcgtaactgc aatcaccgca tagaatggat tcacgtccag
     2521 caacaaacca aacgcgacgc caagtaatga ggcatgagcc agcgtatcac cgaaataaga
     2581 catacgacgc cagactacaa acgaacccag cggacccgcg gcacaggcga gcatgatccc
     2641 ggctaaccaa ccgggaaata ataattcaat catgagcgat catttccccg acgcaaaaca
     2701 attcgtccct gtaaatcgtg acgatgatta tgatgatggc gatagatacc cagttgttca
     2761 gcaccacgag gaccaaacat agaaataaac tccggatgca gggaaacaac ttccggtgtg
     2821 ccggaacaac aaatgtggtg attcaggcaa agcacttcat cggtttttgc cattaccaga
     2881 tgcagatcgt gagataccat taaaacgcca caatccagtt cgtgacgcag ttggtcaata
     2941 aggtcatata acgccacctg accattcaca tccacgccct gagtgggttc atccagcact
     3001 aataattgcg gtcgatttaa caatgctcgc gctaacagta cacgctgcgt ttcgccaccc
     3061 gagagctttt gcatcggtgc gttaatcaga tgcccggcct ggacacgttt cagtgcaggc
     3121 aaaatatctt ctttatgtgt gccagggcgt aagcgtaaaa aacggtttac ggtcagtggc
     3181 aacgtggtgt cgagatacag cttctgcggt acatagccga tgcgcagttt tccgttgcgc
     3241 ttgataaccc cttcatcggg tgttaccagc ccgagcacta cccgtaccag tgtcgactta
     3301 cctgcgccgt ttggcccaag taaagtcaaa atttttccag gtttaagttc cagcgacaca
     3361 tcagagagga cgcggcgttg gccaaaagaa accgagacat tttccaggga aaccagactt
     3421 gtcatgttaa ttttagtctt gcagtagtca tgaaatgtta taatatcaca gttctcatat
     3481 tcattacgat gattggtcgc attatgttac ataaaaaaac gcttcttttc gcagcattat
     3541 ccgccgctct ctggggcggt gcaacacagg ccgcagatgc cgccgttgtc gcttcgctta
     3601 aacccgttgg gttcatcgct tctgccattg ctgatggggt aacagaaacg caggttttac
     3661 tgcctgacgg cgcttcagaa catgattatt cactgcgtcc atcggatgta aaacgcttac
     3721 agaacgcgga cttagtcgtt tgggttggcc cggagatgga agcgttcatg caaaaaccgg
     3781 taagtaaatt accagaagcg aaacaggtaa cgattgcgca gctagaagat gtgaaaccgc
     3841 tgctgatgaa aagtattcac gacgatgatg atgatcacga ccacgcggaa aaaagtgacg
     3901 aagatcacca tcacggcgat ttcaacatgc atctttggct ttccccagag atagcgcggg
     3961 ctacagcggt tgcaatccat ggaaaattag tggaacttat gccgcaaagt cgagccaaac
     4021 ttgacgccaa cctgaaggat tttgaggcac aattagcctc aaccgaaacg caggttggta
     4081 acgagctcgc gccgctcaag gggaaaggtt atttcgtttt tcacgatgct tacggctact
     4141 tcgaaaaaca gttcggactg acaccgcttg gtcattttac cgttaaccct gagattcaac
     4201 ctggcgcgca gcgtttacat gaaataagaa cacagttggt tgagcaaaaa gcaacctgcg
     4261 tttttgctga gccacagttc aggccagcgg tcgttgagag cgtcgcacga gggacatccg
     4321 ttcgtatggg aacgttggat cccctgggga cgaatatcaa actgggtaaa acaagctatt
     4381 cagaattcct gaatcaatta gccaaccagt atgcgagctg cctgaaagga gattaatgag
     4441 gaagtgatta cgtgcaacag atagcccgct ctgtcgccct ggcgtttaat aatttaccgc
     4501 gaccacaccg cgttatgtta gggtcgctca ccgttcttac tctggccgtc gctgtctggc
     4561 ggccctatgt ttatcaccgc gatgccacgc caattgtcaa aaccattgag ctggaacaga
     4621 acgaaattcg ttcgctctta cctgaagcca gtgagccgat tgatcaagct gcacaagaag
     4681 atgaagccat tccccaggat gaactggatg acaaaatcgc cggtgaagcg ggcgtgcatg
     4741 aatatgttgt ttccactggc gatacgctaa gcagcattct caatcagtat ggtattgata
     4801 tgggtgatat cacccaactg gctgctgccg acaaagaatt gcgtaacctg aaaatcggtc
     4861 aacaactctc ctggacatta accgcggacg gcgaactgca gcgcctcacc tgggaagtgt
     4921 ctcgtcgtga aacccgaacc tatgaccgta ctgccgctaa cggttttaaa atgaccagcg
     4981 aaatgcagca aggagagtgg gtcaacaatc tgctgaaagg taccgtcggg ggaagctttg
     5041 ttgccagcgc cagaaacgcc ggtttaacca gcgccgaagt gagcgcagtg attaaagcca
     5101 tgcagtggca aatggatttc cgcaaactga aaaaaggcga tgaatttgcg gtgttaatgt
     5161 cacgagaaat gcttgatggt aaacgtgagc aaagccagct gctgggcgta cgtttgcgtt
     5221 cagaaggtaa agattattac gcaatccgcg ctgaagatgg caaattctac gatcgtaacg
     5281 gtactggtct ggcgaaagga ttcttgcgat tcccgacggc gaaacagttc cgtatctcat
     5341 ctaactttaa cccgcgtcgt actaatccgg tgaccggtcg cgttgcgcca cacagaggtg
     5401 ttgattttgc catgccacaa ggtacgccag tgctttcagt gggtgacggt gaagtggtgg
     5461 ttgccaaacg cagtggcgca gcaggttatt atgtggctat tcgtcatggt cgcagctaca
     5521 ccacgcgtta tatgcacttg cgcaagatcc tggtgaaacc gggacagaag gtgaaacgtg
     5581 gcgaccgtat cgcgctttcc ggtaataccg gacgttcaac cgggccgcat ctgcactatg
     5641 aagtatggat aaaccagcag gccgtaaacc cgctgacggc aaaactgccg cgtaccgaag
     5701 ggctgaccgg ctccgatcgt cgcgaattcc tggcgcaggc caaagagatt gtgccgcagc
     5761 tacggtttga ttaattaaca tccattcgca gccggtacgc agtcagtacc gg
//
LOCUS       DAZUCC010000255         5796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZUCC010000255 DAZUCC010000000
VERSION     DAZUCC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.256"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            92..322
                     /gene="chaB"
                     /locus_tag="ACHY09_004516"
     CDS             92..322
                     /gene="chaB"
                     /locus_tag="ACHY09_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFS3887388.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            480..1175
                     /gene="chaC"
                     /locus_tag="ACHY09_004517"
     CDS             480..1175
                     /gene="chaC"
                     /locus_tag="ACHY09_004517"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFS3887389.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(1219..1572)
                     /gene="ychN"
                     /locus_tag="ACHY09_004518"
     CDS             complement(1219..1572)
                     /gene="ychN"
                     /locus_tag="ACHY09_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFS3887390.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1758..3152
                     /gene="ychO"
                     /locus_tag="ACHY09_004519"
                     /gene_synonym="iatA"
     CDS             1758..3152
                     /gene="ychO"
                     /locus_tag="ACHY09_004519"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFS3887391.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDAFSQQVNQHV
                     ESWLSAWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3153..3803)
                     /gene="narL"
                     /locus_tag="ACHY09_004520"
     CDS             complement(3153..3803)
                     /gene="narL"
                     /locus_tag="ACHY09_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFS3887392.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3796..5592)
                     /gene="narX"
                     /locus_tag="ACHY09_004521"
     CDS             complement(3796..5592)
                     /gene="narX"
                     /locus_tag="ACHY09_004521"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFS3887393.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSARFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
BASE COUNT         1419 a         1431 c         1527 g         1419 t
ORIGIN      
        1 aagaagagtc tgacatgaaa attcttatgt tttggcaaga gtagatattg ttgaccacac
       61 ttaatgttca actttgtaaa aggagtcaac gatgccgtat aaaacgaaaa gcgatctgcc
      121 ggaaagcgta aagcacgtgc ttccgtctca tgcccaggat atctataaag aagcgttcaa
      181 cagcgcatgg gatcaatata aagataaaga agatcggcgt gatgacgcca gtcgcgaaga
      241 aacagcgcat aaagtggcct gggctgctgt gaagcatgaa tatgccaaag gggatgatga
      301 taaatggcat aaaaaatcgt aaaaccggta gcttagttaa agctattcgt gcggtgttgc
      361 cttgcaagtg gtccgtggat tgcatattgt cccgttagtg gtttcaaaat gagcagtaaa
      421 aatgtccgga agacaccaaa aagttgtcgc agggaagtat gcagtggcgg aggtgtaagg
      481 tgataacgcg tgatttcttg atgaatgccg attgtaaaac ggcatttggt gccattgaag
      541 aatcactctt atggtcagca gaacaacggg cggcttcgct ggcggcgacg ctggcttgtc
      601 gacctgatga gggaccggtg tggatcttcg gttatggatc gttgatgtgg aatccggcac
      661 tggagtttac cgagtcgtgc accggtacac tggttggatg gcatcgtgcg ttttgcctgc
      721 gcctgaccgc cgggcgcggg actgcgcacc agccgggacg gatgcttgca ctgaaagagg
      781 gcggacgcac cacaggcgtc gcctatcgac tgccagaaga gacgctggag caggaactaa
      841 ccctgttgtg gaagcgcgag atgattaccg gctgttatct gccaacctgg tgtcagcttg
      901 atcttgatga tggacgcaca gtaaacgcca ttgtgtttat tatggacccg cgacatccag
      961 aatatgaatc tgacactcgc gctcaggtca tcgcgccgtt gattgcggcg gcgagcggtc
     1021 cactgggaac caacgcacaa tacctgtttt cactggaaca ggagcttatc aaactgggaa
     1081 tgcaggacga tgggctgaat gatttgctgg tatcggtaaa aaaactgctg gcggagaatt
     1141 atccggatgg tgtgttacgg ccgggactcg cctgagtaaa cttccctcat agtggggcgt
     1201 cagacgcccc ctcaaacatt aaaatgtgag cactttatcg gctgacagcg tccattgcgc
     1261 cagttccaca agcgtaccga tttccacccc atcaatcaga ggaagtgtac taatcccgcg
     1321 cccgtcggta caggttttgc acaatttcac cggtacattc tgagcggtaa ggatctccag
     1381 catttgctga atgttgtagc cttcccctgg tttttgcccg cgcaacccgg ctgtgaccgc
     1441 atcagacatc aggaacagac gcagatccag attgctctcc tgctctcgta acgcaatggc
     1501 cagccgcaag ctgttaaata aggattcgct cccgtaaggt gcgccattgg caacgatcac
     1561 gattttttgc attatttact cctgtattca gggaattaga cactcatctt ctatcttact
     1621 gcttctgcag cgtctgacca atcggtcaca tttttaagga ttttcctgaa agcgcgagaa
     1681 aatacgacaa aagttgccag taatcgttat tctttagggc tatggttttt cattttttac
     1741 cggaagttac cgacgttttg agccgtttcg ttcctcgcat tattccgttt tatttactct
     1801 tgctggcggc aggcggtaca gctaacgcac aatctacctt cgagcaaaaa gcggcaaatc
     1861 cctttgataa taacaatgat ggtctgccgg atttaggcat ggcacctgaa aatcatgatg
     1921 gggaaaaaca ctttgctgaa attgtgaaag atttcggcga aaccagtatg aatgataacg
     1981 ggctggatac tggcgagcag gcaaaagctt tcgcattggg aaaagtccgc gacgcgttta
     2041 gtcaacaggt taatcagcac gtagagtcgt ggctatcagc gtggggaaat gccagtgttg
     2101 acgtcaaagt ggataacgaa ggacatttca ccggcagtcg tggaagctgg tttgtgccgt
     2161 tacaagataa tgatcgttat ctcacctgga gccagcttgg tcttactcag caggatgatg
     2221 ggctggtgag caatgtgggc gttgggcaac gctgggcgcg cggcagctgg ctggtgggtt
     2281 ataacacttt ttatgacaac ttgctggacg aaaatcttca gcgagcgggc ttcggtgccg
     2341 aagcgtgggg cgaatatttg cgactatcgg caaactttta tcagccgttt gctgcatggc
     2401 atgaacagac agccacgcag gaacaacgga tggcgcgcgg gtacgacctg acagcccgga
     2461 tgcgcatgcc gttttatcaa cacctcaata ccagtgtcag cgtagaacag tattttggtg
     2521 atcgtgttga tttgtttaac tctggtacgg gttatcacaa tcccgtcgcg ttgagtctgg
     2581 gattaaatta taccccagtg ccattagtca ctgtgacggc ccagcataaa cagggtgaaa
     2641 gtggcgaaaa tcaaaataac ctcgggctga atcttaatta ccgctttggt gtaccgctca
     2701 aaaaacaact ttctgcgggc gaggttgccg aaagtcagtc gttacgtggt agtcgctatg
     2761 acaatccgca gcgaaataat ctaccgactc ttgagtaccg acagcgaaaa acgttaacgg
     2821 tgtttctggc gacaccgccg tgggatctaa aacctggcga aacagtgccg ctgaaattac
     2881 aaatccgcag tcgttacggt attcggcaac tgatttggca gggcgatacg cagatattaa
     2941 gtttgacgcc gggcgcacaa gccaacagtg aggagggctg gacgctgatc atgcctgact
     3001 ggcaaaacgg ggaaggcgca agcaatcact ggcgattgtc agtggtggtg gaagataacc
     3061 aggggcagcg tgtctcctcc aatgagatca cgctaacgct tgtcgaaccg ttcgacgcat
     3121 tgtcaaacga cgaactgcgc tgggaaccgt aatcagaaaa tgcgctcctg atgcacccat
     3181 accgcagctt ccacgcgaga ctttagcttc attttcttca gcatgtgctt gacgtgaact
     3241 tttactgtgc tttcggtgat gtccaggcgg cgggcaatca tcttgttcgg caaaccctgg
     3301 gcaattagct tgagaatatc gcgctcgcgt ggggttaact ggttaacatc gcgctcagta
     3361 gtggcacggt tagcgcgcaa gctggcggcc agaacaggcg ttaatgcttc gcttaatacc
     3421 atttcgccag cagctgcctg atgtaacgct ttcagcagat cttccggttc catatctttt
     3481 aacagatagc catccgcgcc gcgtttcagt gcggtgacca catcttcttc atggttagag
     3541 acgctgaata ccacaatgcg cccggagagg gacttttcgc gcagtttatc cagcgtttcc
     3601 agaccgttca tgccgggcat attgagatct aacaggatca gatcgggatc aagagactcc
     3661 gccagttcaa taccctgttc gccattactc gcttcgccaa ccacggtgat atctggtgcc
     3721 atactgataa gctgttttac gccagttcgc agcatcgggt gatcgtcaat cagcaggata
     3781 gtagccggtt cctgattact catgggtatc tccttggacg tctgtgaaag ttttttcggg
     3841 aataaaggtg accaccactt cggtgccacc tgattcacga cggcggacgc ggcaatcgcc
     3901 ccgtaaactt tgcgcacggt cgcgcattat tatcatgccg tagtgattgc tgcggatggc
     3961 attttcaggc acgccgcagc cgttatcctg gacggtcagt ttgacctgat tatcgttttg
     4021 cgccactgtc accacgacct cactcgcttg cgaatgtttg agggcgttac ttaatgcctc
     4081 acgggcaatt tgcaacagat ggattgcctg atgcgaaggc accagacgag gcggcaattg
     4141 ataatccagc ttcaccggga agccaaacct ggcgctgtac tcttcgcaac tcgcctccag
     4201 cgccggacgt aatccaggct cggtgagctg caagcggaat gtggtgagca attcacgcaa
     4261 ctgcgcccag gatgcattca gttcgttacg gatctgactt aacagttcgc ggctgctttc
     4321 tggcagcgca tcgccctgca tctgtaaaca actcacctgc atcttcatgc aagagagaga
     4381 ttgggcaata gaatcatgca gttcgcgcgc aatggtggca cgctcttcca tcacgatcaa
     4441 ctgttgctga cgttcctgat ggcgatccag cgcaagcgtg gcggtgagtt gttcaaccag
     4501 ggtatccacc agttgttgtt gatcatgact aagatgacgc ccctgcggca gggttgccag
     4561 caaaataccg tactgcgtat gagagtcagc cagccgccac ttcagggttg tgccgcgatc
     4621 gccaacgggg agtacaccgc gcgggcagag ctgacagcct ttatcatcgc aggtcatatc
     4681 tggctggcag gtaaactcct gatgattctc ttcatcatca gtgtcataca cccgcagttc
     4741 gatatcacgt agcagggtta aattctgtaa gccgttgagt acaggtgaca ggcgttcaca
     4801 cagcggggcg cgggaatgca aacggcggtt ggcctgccat aaaaaagaga ggatctgatt
     4861 tttatgttcc agcccggcgg ttttctcctg aacccgctgc tcaagtacgg cgtaactttc
     4921 cgccagttct gcagacatat tgttcaacgc agttccaagc atagccattt cgttgcgccc
     4981 gctgatgttc gcgcgttggg taaaatcgcg atggctgacg gcactcgcca ttgccaacaa
     5041 ctgccgccac ggttgtagca gtcgcgcccg caaccagata atagtgaaca ccagtaaaag
     5101 tgccataaat accgccatta cccgatggac cagcaccact gtctcaatgc gcatttccgt
     5161 ggtgcggtca aaaccagata ccagttgatc aagcccggca acaaactggc tgacatccgc
     5221 tgacaccgtt tcgcggtttt gtgcacgcat tagcgcaggg atcagttcat tacgccagta
     5281 atcttgtaaa ccctgtaatt gcgccagttg tccgtctcgt tctgctgctc gggttaactc
     5341 ggcgctaaat gctgtttgtt ccatctcttt aattaagggc ttgtctttct cgcttaatgg
     5401 cactgctgcc aacagacggt aactttgcat gcgcagcgat cccgctttgt tgatcgcatg
     5461 ggcgctgccc tgaacgcctt gcaccagcca gccagaaact gccatccctg ccagtccaat
     5521 agcagtagaa agcaacacaa taagcgcaac ctgattaacc agggtgagcg gagagagaca
     5581 acgtttaagc atgcaaacct cttccttcag gctttaaatg agcaataacc ttaatgaatg
     5641 tgacgataca ttctggaatg gcagtattct cggctattgg ctgaagtata cccatacccg
     5701 gaaagagtta ctccttattt gccgcgtggt tagtcgcttt acatcggtaa gggtagggat
     5761 tttacagcac cgtgaaaaat ctcataattt ttatga
//
LOCUS       DAZUCC010000256         5766 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZUCC010000256 DAZUCC010000000
VERSION     DAZUCC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.257"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..1405
                     /gene="adeQ"
                     /locus_tag="ACHY09_004522"
                     /gene_synonym="yicO"
     CDS             71..1405
                     /gene="adeQ"
                     /locus_tag="ACHY09_004522"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFS3887394.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISSVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1458..1910
                     /gene="yicN"
                     /locus_tag="ACHY09_004523"
     CDS             1458..1910
                     /gene="yicN"
                     /locus_tag="ACHY09_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFS3887395.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2121..3311
                     /gene="nepI"
                     /locus_tag="ACHY09_004524"
     CDS             2121..3311
                     /gene="nepI"
                     /locus_tag="ACHY09_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFS3887396.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3352..3645)
                     /gene="yicS"
                     /locus_tag="ACHY09_004525"
     CDS             complement(3352..3645)
                     /gene="yicS"
                     /locus_tag="ACHY09_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFS3887397.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3867..4685
                     /gene="nlpA"
                     /locus_tag="ACHY09_004526"
     CDS             3867..4685
                     /gene="nlpA"
                     /locus_tag="ACHY09_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFS3887398.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4689..5612)
                     /gene="yicL"
                     /locus_tag="ACHY09_004527"
     CDS             complement(4689..5612)
                     /gene="yicL"
                     /locus_tag="ACHY09_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFS3887399.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
BASE COUNT         1373 a         1304 c         1488 g         1601 t
ORIGIN      
        1 tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat ctcctccaat
       61 ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag ggacgctttc
      121 gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga ttgcggggat
      181 gaccactttt ttaaccatgg tatacatcgt ttttgtgaat ccgcaaatcc tcggcgcggc
      241 acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta tcggcagtat
      301 tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg ggctgaacgc
      361 cttctttgcg ttcgtggtcg tgggggcgat gggcatctcc tggcagaccg ggatgggcgc
      421 gatattctgg ggcgcagttg ggctatttct gctcacgctg tttcgtatcc ggtactggat
      481 gatctccaac attccattaa gtttacgtat tggtatcacc agcggaatcg gattatttat
      541 cgccttaatg ggattaaaaa atactggcgt tattgtcgcc aataaagaca ccctggtgat
      601 gattggcgat ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac
      661 cgtgttgtca tcacgtcatt ttcatgccgc ggtgcttgtt tctattgtgg tgacgtcttg
      721 ctgtggatta tttttcggtg atgttcattt tagcggcgtc tattccattc cgcctgatat
      781 tagcagcgtc attggtgaag tggatttgag cggcgcgtta tcacttgaac tcgccggtat
      841 cattttctcc tttatgctga tcaacctatt tgattcatca ggaacattaa ttggtgtaac
      901 tgataaagct ggcttaatag atagtaacgg taaattccct aatatgaata aggcgctgta
      961 tgttgatagc gtcagttcgg tggcgggtgc gtttatcggc acctcgtctg ttaccgccta
     1021 tattgaaagt acttctggtg tggcagtcgg tggtcgcacg gggctgactg cggttgtggt
     1081 cggcgttatg ttcctgttgg ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta
     1141 cgcaaccgcc ggagcgttaa tctttgttgg cgtgttgatg acctcaagtc tggcgcgcgt
     1201 taactgggat gattttaccg aatcggtacc tgcgtttatt accacggtga tgatgccctt
     1261 tactttctcg atcaccgaag ggattgcact cggctttatg tcgtactgca tcatgaaagt
     1321 gtgcaccggg cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag ctctgtttgc
     1381 actgaagatt attctggtgg attagtaact ggcgagggtt tccagatatc atgagttctg
     1441 attacgcagg agaactcatg atctggataa tgctcgccac gctggcggta gtgtttgtgg
     1501 ttggttttcg ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc agcgatcggc
     1561 tgaacatcga tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag tcagccggtg
     1621 acgaattttt acgttatttg catcgtccgg atgagtcgca cctgcaaaac gccgcgcaag
     1681 tgttgctcat ctggcaaatt gtcattgtcg atggtagcga acagaacttg ctgcaatggc
     1741 atcggatttt acaaaaagcc cgccttgccg ctcccattac cgatgctcag gtcaggctgg
     1801 cgctgggttt tctgcgcgaa accgaacctg aaatgcagga tattaatgct tttcagatgc
     1861 gctataacgc gttctttcag cctgccgagg gcgttcactg gctgcattaa aaccgcttcg
     1921 tcttatcagc ctacagaccg cgcctgacct gtagggtgga taaggcgttc acgccgcatc
     1981 cggtaatctt tgccctgttc cgtttcgatc ttaactccag ctcaagcaac accgtaaaag
     2041 ttgcaaaaac gttaaatacg tcacacattt caatgttaaa gtggtcggct ttccctctga
     2101 aacatgccac gggtaacacc atgagtgaat ttattgctga aaaccgcggc gcggatgcca
     2161 tcacccgacc gaactggtca gccgttttct cggtggcgtt ttgcgtcgcc tgtctgatta
     2221 tcgttgagtt tttgcctgtc agtttgttga cgccaatggc ccaggattta ggcatttcgg
     2281 aaggggttgc cgggcaatcg gtgaccgtga ccgcctttgt ggcgatgttt gccagtttgt
     2341 ttattaccca gaccattcag gccactgacc gccgctacgt tgtcattctg ttcgccgttt
     2401 tgctgacgct ttcctgtttg ctggtttcct ttgccaactc attcagtctg ctgttaatcg
     2461 gtcgcgcctg tctggggctg gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca
     2521 tgcgtctggt accgccgcgt acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt
     2581 ctattgcgct ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt atcggttggc
     2641 gcaatgtctt taatgcggcg gcggcgatgg gtgtgctgtg tattttctgg attatcaaat
     2701 cactgccttc gctgccaggt gaaccttcgc atcagaaaca aaatactttc cgcttattac
     2761 aacgtccggg agtgatggcc gggatgatcg ccatctttat gtcctttgcc ggacagtttg
     2821 cttttttcac ttatattcgc ccagtgtata tgaatctggc gggattcggt gttgatggct
     2881 taaccctggt gctgctgagt tttggtatcg ccagctttgt cgggacatcg ctttcgtcgt
     2941 ttattctcaa acgttcggtg aaactggcct tagcgggcgc accgtttgtg ctcgccctga
     3001 gcgcgctggt actgacgctg tggggaagcg ataaaattgt tgctactggt gtggcgatta
     3061 tctgggggct gacctttgct ttaattcccg ttggttggtc aacgtggatc actcgctcat
     3121 tggccgatca ggcagaaaag gctggatcta ttcaggtggc cgttattcag cttgccaata
     3181 cctgcggtgc ggcaatcggc ggttatgcgt tggataacat tggtctgact tcgccgctga
     3241 tgttgtccgg cacattgatg ttgctgactg cattgttggt gactgcaaag gtgaaaatga
     3301 agaaatcctg atatcaggta aagaagcgtt ggtcagtggt tgttgccgtt cttacatatc
     3361 cgggcattct accttaccca acgcttccca ataattgctc tgattattac gttccatcgc
     3421 cacgatggcc tcgcgtatat gttgtttatt gttttcatct gacaacatta acttttccca
     3481 cgcttcatct gataatgacg cctgtggcgt gcaaatttta cgtaaatctt gcaataccgt
     3541 ggttttctct tttgccgact gtagagaact gaatggcgac tcggcctgag aaatggctgg
     3601 aaaaatcagg aatacggtaa tcatgagtag catcgttggc ttcatagcgg caaccttctg
     3661 caaatgtgca tcgaatgaag cataagtgta gtttgctttt gccgctctca tcatgctgta
     3721 cgaaaggtgt ttgcgtggtc agtaagaagt gccagacttt atattccact tttattcctt
     3781 tttattctta tcgatagcgt ttcgtttttt aaactgcagc gaccttaccg ctatagtcag
     3841 gtaatcatta ataaaaggat aaaaaaatga aactgacaac acatcatcta cgggcggggg
     3901 ccgcattatt actggcagga attctgctgg caggttgcga ccagagcagc agcgatgaaa
     3961 agcacattaa agtgggcgtt attaatggcg cagaacaaga tgtcgcggaa gtcgctaaaa
     4021 aggtggcaaa agagaaatat ggcctcgatg ttgaactggt aggatttagc ggttcgttgc
     4081 taccgaacga tgcgacaaat catggcgagc ttgatgcgaa cgtcttccag catcgaccct
     4141 ttcttgaaca agataatcag gcgcatggtt ataaactggt agcggtggga aatacgtttg
     4201 tcttccccat ggcgggttat tccaaaaaaa tcaaaaccgt ggcgcaaata aaagaaggcg
     4261 cgacagtggc gatccccaac gaccctacca accttggtcg cgcgctttta ctgttgcaaa
     4321 aagagaagct aattactctg aaagagggaa aagggttact acctaccgcg ctggatataa
     4381 ccgataatcc acgccatttg cagataatgg agctggaagg ggcacagttg ccacgtgtac
     4441 tggacgaccc gaaagtggat gtagcgatta tcagcaccac ttacatccag caaacaggac
     4501 tttctccggt taacgacagc gtatttattg aagataaaaa ttcgccgtat gtgaatattc
     4561 tggtggcacg ggaagataat aagaatgcag aaaacgtgaa ggaatttctg caatcttatc
     4621 aatcacccga agtcgctaaa gcggcagaaa ccatctttaa cggaggcgcg gtgcctggct
     4681 ggtaattctc actcatgccg cgccggacga tttatttttc tggcgcggcg gcgggaatcc
     4741 attgaaatca aaatcaccga tgacagaatc agcagcgttc ccagccagtc cggcaatgtg
     4801 aaggtgatcc ccagcaacag caaagagagc agcgcgctac ttaatggttc tgcacagctt
     4861 aaaatgctgg cttttggacc gccaattaat tgtgctcctt tcaggtacag actaaatgtc
     4921 agggacgtac caatgaccac caaataaaaa aacgccagaa tcaaactgcc gttaacgaca
     4981 aagtttgtcc cttgtctggc ataaaagggc agcaggatca gaccgccaat cagcatactc
     5041 cagccgacga ccggtaacgt gccatagcgg gcaatcagcg ttgagggata ggtggtatag
     5101 aatgcagcag caaatgccga ggcaatgccc cagaataacg cggcaggaga gatcgataat
     5161 gacgtcggat taccgtgtgt caccaataaa aaagtcccga tcagcgatgt caaaatagcg
     5221 cagaaaacca gaatgcccgg gcgggattta cgcaccagtg agaaccaggc gacgataata
     5281 gtcggtgaga ggaattgcag caccgttgcc gtggctgcgt tcgatttttc tatggttagc
     5341 aaaaaagtga gctgtacggt tagcgcgcca accacggaaa aaatcagtag gctaatggca
     5401 tctttgtgat tgttaataat agaaaagatt ttatcaccat gaacaaatga tagcgtcagc
     5461 agaatcaaac cggcgaatat caaacgcgtc atagtcaaaa actgcgacga catctggctt
     5521 tgctccatga tgtattgcgc gcagacccct gaacttcccc acaacacggc ggcaatcaga
     5581 acgttcagca tcccctttct ggtggaaccc atttttccct ctgcatactg ttttgtcttt
     5641 tcatcaatat acgttaagga taccactacc gatgacggtg agggagtaga gtaatcaatc
     5701 agttttagtg aatggaggaa ataatgacat ataacaatta ttcatatggt atttaaaaaa
     5761 actcaa
//
LOCUS       DAZUCC010000257         5744 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZUCC010000257 DAZUCC010000000
VERSION     DAZUCC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.258"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            28..1017
                     /gene="ldhA"
                     /locus_tag="ACHY09_004528"
     CDS             28..1017
                     /gene="ldhA"
                     /locus_tag="ACHY09_004528"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFS3887400.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFEQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1128..1550
                     /gene="hslJ"
                     /locus_tag="ACHY09_004529"
     CDS             1128..1550
                     /gene="hslJ"
                     /locus_tag="ACHY09_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFS3887401.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1547..1813)
                     /locus_tag="ACHY09_004530"
     CDS             complement(1547..1813)
                     /locus_tag="ACHY09_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFS3887402.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTTIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2087..5611
                     /gene="nifJ"
                     /locus_tag="ACHY09_004531"
     CDS             2087..5611
                     /gene="nifJ"
                     /locus_tag="ACHY09_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS3887403.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPDSVRTVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSVDEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLKDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1428 a         1479 c         1493 g         1344 t
ORIGIN      
        1 tgattcaaca tcactggaga aagtcttatg aaactcgccg tttatagcac aaaacagtac
       61 gacaagaagt acctgcaaca ggtgaacgag tcctttggct ttgagctgga attttttgac
      121 tttctgctga cggaaaaaac cgctaaaacc gccaatggct gcgaagcggt atgtattttc
      181 gtaaacgatg acggcagccg cccggtgctg gaagagctga aaaagcacgg cgttaaatat
      241 atcgccctgc gctgtgccgg tttcaataac gtcgaccttg acgcggcaaa agaactgggg
      301 ctgaaagtag tccgtgttcc agcctatgat ccagaagccg ttgctgaaca cgccatcggt
      361 atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg tgacgccaac
      421 ttctctctgg aaggtctgac cggctttact atgtatggca aaacggcagg cgttatcggt
      481 accggcaaaa tcggtgtggc gatgctgcgc attctgaaag gttttggtat gcgtctgctg
      541 gcgttcgatc cgtatccaag tgcggcggcg ctggaactcg gtgtggagta tgtcgatctg
      601 ccaaccctgt tctctgaatc agacgttatc tctctgcact gcccgctgac accggaaaac
      661 tatcatctgt tgaacgaagc tgccttcgaa cagatgaaaa atggcgtgat gatcgtcaat
      721 accagtcgcg gtgcattgat tgattctcag gcagcaattg aagcgctgaa aaatcagaaa
      781 attggttcgt tgggtatgga cgtgtatgag aacgaacgcg atctgttctt tgaagataaa
      841 tccaacgacg taattcagga tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg
      901 tttaccgggc atcaggcatt cctgacagca gaagcgctga ccagtatttc tcagactacg
      961 ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc cgaacgaact ggtttaatct
     1021 tgccgctccc ctgcattcca ggggagctga ttcagataat ccccaatgac ctttcatcct
     1081 ctattcttaa aatagtcctg agtcagaaac tgtaattgag aaccacaatg aagaaagtag
     1141 ccgcgctcgt tgcgctaagc ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg
     1201 taaccccaga acagctacag catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg
     1261 tgaccagcga taaaaatccg ccagaaatca gctttggtga aaagatgatg atttccggca
     1321 gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa tggtgaactg accgccaaag
     1381 ggctggcaat gacccgtatg atgtgcgcta acccgcagct taatgaactc gataacacca
     1441 ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg
     1501 cgaccgcgaa acagacatta acttataagc tggcggattt aatgaattaa tagctgccac
     1561 agctcccggc ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat gcacacatcc
     1621 caatcgtcgt accatccagt tgacgggcaa cagaaagcga accgccgatc attgcacaat
     1681 ttgcttctcc actactggac atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg
     1741 cctgctgaac aggttcacta ctacacgccg acaacaataa agcggcacac cctacccaaa
     1801 acgctgctcg catcttttct tcctctgatc ttcaagccaa acgacaccgc cataaataat
     1861 aggcagcaca gagggcggcg tcgagagctg tcctgagcgt tgccccgcat ttttactttt
     1921 ttatggctat ttttttgccc tctgtttgat caaaacattc attacgctga tgtgggggac
     1981 acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt gaaggattac tactgggcgc
     2041 gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga ttactattga
     2101 cggtaatggc gcggttgctt cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc
     2161 tattaccccc agttccacga tggcagaaca ggctgatgcc tgggccggaa acggcttgaa
     2221 gaacgtttgg ggagacacac cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc
     2281 taccgtgcat ggcgctttgc agacgggtgc cctctcaaca tcgtttacgt catcgcaggg
     2341 tttgctgctg atgatcccga cgctgtacaa actggcaggc gaactgacgc catttgtcct
     2401 gcatgtagcg gcacgtaccg ttgccacaca tgcactctct atttttggcg atcattccga
     2461 cgttatggcg gtgcgccaga cgggttgcgc gatgttgtgt gcggctaacg tccaggaagc
     2521 gcaagacttt gctctcattt cgcacatcgc aacgctgaag agccgcgtgc catttattca
     2581 tttctttgat ggtttccgca cgtcccacga aatcaataaa attgtcccgc tggccgatga
     2641 cactattctt gatctcatgc cgcaggccga aattgatgct catcgcgccc gggcgctcaa
     2701 cccggaacat ccggtgatcc gcggtacgtc cgccaatcct gacacttatt tccagtctcg
     2761 cgaagccacc aacccatggt acaacgcagt ctatgaccat gttgaacagg cgatgaatga
     2821 tttcgctgcc gcgacaggtc gccagtatca gccgtttgag tattacgggc atccacaagc
     2881 ggaacgggtg attatcctga tgggctctgc cattggcacc tgtgaagaag tggttgatga
     2941 attgctgacc cgtggcgaaa aagtcggcgt gctgaaagtt cgcctgtacc gccccttctc
     3001 cgccaaacac ttactgcaag cgttgccgga ctctgttcgg acagtggcgg tgctggatcg
     3061 caccaaagag ccaggtgcgc aagcggaacc cctgtacctg gatgtgatga ccgcactggc
     3121 agaagccttt aataatggcg agcgtgaaac cctgccccgt gtcattggtg ggcgctatgg
     3181 tctttcatcc aaagaatttg gcccggactg tgtactggcg gtatttgccg agctcaacgc
     3241 ggctaaaccg aaagcgcgct ttacggtagg tatttacgat gatgtgacca atctgtcact
     3301 gccgttgccg gaaaacaccc tgccaaactc ggcgaaactt gaagccttgt tttatggcct
     3361 tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc aagattatcg gtaattccac
     3421 gccgtggtac gcacagggct attttgttta cgactccaaa aaggcgggcg gcctgacggt
     3481 ttctcacctt cgcgtgagcg agcagccgat tcgttccgct tatctgattt cccaggctga
     3541 ttttgttggc tgccaccagt tgcagtttat cgataaatat cagatggctg aacgtttaaa
     3601 acctggcggc attttcctgc tcaacacgcc gtacagcgta gatgaagtat ggtcgcgctt
     3661 gccgcaggaa gttcaggccg tgttaaacca gaaaaaagcg cgcttctatg tgattaacgc
     3721 ggcgaaaatc gcccgcgaat gtggtctggc ggcccgtatt aataccgtca tgcagatggc
     3781 ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc
     3841 gattgccaaa agttacagta gcaaaggtca ggatctggtg gagcgcaact ggcaggctct
     3901 ggcgctggcg cgtgaatccg tagaagaagt tccgttgcag ccggttaatc cgcacagcgc
     3961 caatcgaccg cctgtggttt ccgatgccgc tcctgatttc gtgaaaaccg ttaccgcggc
     4021 gatgctcgcc gggcttggtg atgccctccc cgtttcggcg ctgccgccag acggcacctg
     4081 gccgatgggc accacgcgct gggaaaaacg caatatcgcc gaagagatcc ccatctggaa
     4141 agaggaactc tgtacccaat gtaaccactg cgtcgctgct tgcccacatt cggctattcg
     4201 cgcaaaagtg gtaccgcctg aagcgatgga aaacgcccct gccagcctgc attcgctgga
     4261 tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg caggtggcac cggaagattg
     4321 caccggctgt aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga atccagagat
     4381 taaagccatc aatatgatgt cgcgcctgga acatgtcgaa gaagagaaaa tcaattacga
     4441 tttcttcctc aacctcccag aaatcgaccg tagcaaactg gaacgtattg atattcgtac
     4501 atcacagctg attacaccgc tgtttgaata ttcaggtgct tgctccggtt gtggcgagac
     4561 gccgtatatt aaattgctga ctcagctcta tggcgaccga atgttgatcg cgaacgccac
     4621 cggctgttct tcaatttatg gcggtaacct gccctctaca ccgtatacta ccgatgccaa
     4681 cggtcgtgga ccggcatggg cgaactcgct gtttgaagat aacgccgaat ttggccttgg
     4741 tttccgcctg acggtcgatc aacaccgtgt ccgcgtgttg cgtctgctgg atcaatttgc
     4801 cgataaaatc ccggcggaat tactaactgc gttgaaatca gacgccacgc cagaggttcg
     4861 tcgtgaacag gttgcagctt tacgccagca actcaaagat gttgccgaag cacatgaact
     4921 gctacgtgac gcagatgcac tggtagaaaa atcaatctgg ctgattggtg gtgatggctg
     4981 ggcttacgat atcggctttg gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa
     5041 cattctggtg ctggatacgc aatgctattc caacaccggt ggtcaggcgt cgaaagcgac
     5101 accgctgggt gcagtaacta agttcggcga gcacggcaaa cgtaaagcgc gtaaagatct
     5161 tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg cagatttctc tcggcgcgca
     5221 gctcaaccag acggtgaaag cgattcagga agcggaagcg tatccggggc catcgctgat
     5281 catcgcttat agcccctgtg aagagcatgg ttacgatctg gcactcagtc acgaccagat
     5341 gcgccaactc actgccaccg gcttctggcc gctatatcgc ttcgatccac gtcgagccga
     5401 tgaaggcaaa ctgccgctgg cattggattc acgcccgccg tcagaagcac tggaagaaac
     5461 gttacttcac gagcagcgtt tccgtcgtct gaattcgcag cagccagaag tggcagaaca
     5521 gttatggaaa gatgctgcag ctgatttgca aaaacgctat gacttcctgg cacaaatggc
     5581 cggaaaagcg gaaaaaagca acaccgatta aatgcactgg ataaggatta tccaattcta
     5641 aaaaaaagcc cggacgactg ttcgggcttg tctttttata tgttggaaaa tcagtagcaa
     5701 tgcaatggcc caacagaaaa taaaatctgt gacaataaag gcat
//
LOCUS       DAZUCC010000258         5703 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZUCC010000258 DAZUCC010000000
VERSION     DAZUCC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.259"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..137)
                     /locus_tag="ACHY09_004532"
     CDS             complement(<1..137)
                     /locus_tag="ACHY09_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887404.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     D"
     gene            complement(184..609)
                     /locus_tag="ACHY09_004533"
     CDS             complement(184..609)
                     /locus_tag="ACHY09_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS3887405.1"
                     /translation="MQYAKSVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(1183..2295)
                     /locus_tag="ACHY09_004534"
     CDS             complement(1183..2295)
                     /locus_tag="ACHY09_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HFS3887406.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECILSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
     gene            complement(2373..3143)
                     /locus_tag="ACHY09_004535"
     CDS             complement(2373..3143)
                     /locus_tag="ACHY09_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887407.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDISRLATLLISRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEEEIRAVFDAVRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(3188..3674)
                     /locus_tag="ACHY09_004536"
                     /pseudo
     CDS             complement(3188..3674)
                     /locus_tag="ACHY09_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(3634..3855)
                     /locus_tag="ACHY09_004537"
     CDS             complement(3634..3855)
                     /locus_tag="ACHY09_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887408.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(3856..4539)
                     /locus_tag="ACHY09_004538"
     CDS             complement(3856..4539)
                     /locus_tag="ACHY09_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFS3887409.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRFGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(4616..4843)
                     /locus_tag="ACHY09_004539"
     CDS             complement(4616..4843)
                     /locus_tag="ACHY09_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887410.1"
                     /translation="MTRRGINAACGQCPASGRARDGRFYRSRLAPVTVYASLSPFSDE
                     RPSSRFRGIALPSERRRLRYSTVGLTRYRTR"
     gene            4744..4950
                     /locus_tag="ACHY09_004540"
     CDS             4744..4950
                     /locus_tag="ACHY09_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887411.1"
                     /translation="MSPVPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKREAQRH
                     LLLLAKQNPKTTHKHPNKHQKQYK"
     gene            5056..>5703
                     /gene="parM"
                     /locus_tag="ACHY09_004541"
                     /gene_synonym="stbA"
     CDS             5056..>5703
                     /gene="parM"
                     /locus_tag="ACHY09_004541"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFS3887412.1"
                     /translation="MLVFIDDGSTNIKLQWQENDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSDLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQKL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTK"
BASE COUNT         1369 a         1604 c         1482 g         1248 t
ORIGIN      
        1 tcatcgtctg acaggttcca gtcccgggct atgtggcgca catccccgcg ggtcagcacc
       61 acggcaaaaa cacattcact gtccggcacg tctgtattca gcacttcgcg gataatttct
      121 ttaacagtac agtacatgtt ctcgtctcct ttacaggggc ggcattgccg ccccgtccgt
      181 taatcagtcc agtgccgcgt aaatcgcgtt gcattcgggg tgaaattcga tgtggctcca
      241 caactgcgca tcgcgcatcc ggtacaggtg tgtcagtccc gcatcaccgc tgtcgtggta
      301 cagccacaac tggcggttaa tcacaaggga agtcaggatg atacctgcgg catcagcact
      361 cacagtgcgg tcaaaccagt tttcaccgtt gaccagatga aaacggtcgc catccggcat
      421 catgtaaccg ccaccgtccg gcaggcggat gaaatgccag aaccctccct gatattccgg
      481 catcattttt tgcgccagcg cgtacacata cgcctcggca tacagaaaat cgttgccaaa
      541 caggtaaggc agaaaagaaa ggcggtcgca ctcttcaacg ttcagagtga cagatttcgc
      601 atactgcatg ggatttcctc cgttgttcac atattccgcg acaccgggcg gtgccgtgcc
      661 tccatctttg ccgggcctgc agggttgcaa gggcgcaagg gaagccgcag cctgacgcac
      721 acggctgacg ggagttggag ggtgagaccg cgaaatctga gcgggtgaac gaccggaaaa
      781 tcacggcaga cgggaaggag ggaccgggtg accgcagcgg ttcaccttac cttgcaaccc
      841 gcccgcagaa gcccggctac gatggaggca cggcacagcc cgcacacgac gctgaggcta
      901 cagagttgaa aacccacaag gccatgcccc gcatggccgg gtaaagcgga gcctgcggcg
      961 acatcctttt gctctgtccg gggaccggct ggaaaaggcg gagatttttc ccataagcgc
     1021 taacttaagg gttgaaccat ctgaagaatg cgacgcctcg gtgcctcgtt aagacgatgc
     1081 ctcgcgttct tcaattgcgt tttgtaggct gtcagggata ctgtcccacg aatggccacc
     1141 tgtaagctcc agatgaccat ttttgttatt ctccacaacg agttagttct tcttttcgga
     1201 tccggcactt ctggggggga aatccagcga tggctggatt atgtcgtcaa ttaaaaatgc
     1261 ggcgagtaga ttagcaaata tccacgcttt cgcgagttca ggttcctttg cacgcaaagc
     1321 atccaggtgc agcaaacttt tgagccgctt aaaagccagt tcaatttgcc atcgcagacg
     1381 gtaacaatca gccacttgct ctgctgaata ttcatcttcc ggtaatgatg ttagcaatag
     1441 cacatggccc gctgcttcca gcgtttccgc ctgaactact cgtccttttc gacgattctc
     1501 gctgagcagt cgggttttac tgattaatgc tttttcggga ggaagtgata cggcaatgag
     1561 acgtgccgga aagggagctc cggctttttt attacctgaa ttgcctatca ttacagtggt
     1621 ttcaccgttc ttaccgcaat ccagcccgcg cagaaaaccc atcatgtcaa agcgcattcc
     1681 ttctgcagtt aaccagcgca atcctcgcca gtgaacccgg acgatataat cagcttctcc
     1741 aaaagcaagt gagaggatac attcgggacg cgaaccgaat ccccggtcag caatgcgtat
     1801 ctcgtctgcc gtttgcgcaa atcggtccag ccgttcagcg tctctgctgt cggttagctc
     1861 aaaatcagtg aactgacagg tatgaggatc atatcccata tgtagtcgcc attcagcgct
     1921 gccgcccccg ggcgcactga ttgctgttcc atcgacaaga cgcaatctct ttccgcttgt
     1981 acaacccgta actgcggcgc gtacagcaag tgtttgtgcg gcaagtatgc caaaccagtc
     2041 ggcggcattc cgcagccgct tcaggagagc cacgtcagat aatgttgcaa cgtcatggag
     2101 ctgagcccat gcagtgactt cacgtaatga catccccccg gggccgtaag ccagccccag
     2161 acgtagcaga gttgcagcat cacgaatttc gcggcggcgg gttagagccc cggcattacg
     2221 tgccgaagta tccagttctt cgggcttacc aatatgggcc agaattgctg accagttatc
     2281 gtgagagtaa ttcatcggca cgttaaatca tatcaggcgt aataccacaa cccttaagtt
     2341 agcgcttatg agatttttcc ccgccagcgt catcatttcc ggatttttgc cagggcatcc
     2401 gccatgctgc ggatttcggc ccgggcttcc cggacaactt cccgcagaac accacggtca
     2461 aaccaccggc ggggcggttc atccggtata cgcagcacat cgcaggccag cccactggac
     2521 aggatatgct caatacaatc gctgaccaga tacatttcca ccgacgaaaa acgtgctccc
     2581 cggttcacga tggcatcgag caccacgggc agattttcgt catcttcatc aagccagcag
     2641 gggtgatcgc tttccttcac aaccagacga aagcggcgcg tctggcgggt cagcaacgtc
     2701 tgaccggtgg tagtcaacgc cgcacggata cgccgcgcat cttctgccac ccggcaactg
     2761 acactgcata ctgcatcgcg caccgcatca aaaaccgccc tgatttcttc ctccgtcagc
     2821 cattcaccgg catacgggaa agacacctgc gttccgtctg ccggaagtac cggtagcgac
     2881 tgccactggc tgccggacag aacaagttcc gctccggttc cggtcagttt ctgaatggcg
     2941 gaggccaggt gcggcggcag tacgccatca tggcggctga taagtaacgt cgccagccgg
     3001 gatatatcca gtcgcacata aatgctgatc cagcccggat agtgcggatt cagttgttca
     3061 atatccgtct cttccggcca gccctgcgcc agcagcaggt tacgggcgtg gcgacaaacc
     3121 agtgcattca gcgttttgtt catgattaat ggtctccctg aatcgccgcc cgaaggcggc
     3181 gataacatca gtcgttttcg tgtgtgttgt ttttcagcag ggcgcgggcc tgattaacca
     3241 ccgcctgacg gcgggtgacg cattccggga cgacgaggcc gttatcacgg gcggcccact
     3301 cacgtttcca cagtgcctgt tcggcggtct gaatcaggga cgccagcagt tcagccggga
     3361 cggtcacctg gcggttttcg ctcacgttgc tgataatctc catcgccacg ccggaagaaa
     3421 taccggactc agtccgctgg tcttccggga tgtcctccag gcgcaccagg acggtcctga
     3481 tttcatgatc ggacagggaa atatccattc cgtcagcgag ggcctgaatc tcttccgggg
     3541 accagacaac cagcatcaac ggcgtgtctc ccggatactt tgctgccagc gcccggcaga
     3601 gcgtttcgca tgttccgtac atactctctc ccctcaatct ttgaagcagg aatccagttc
     3661 cagaatttcg gtatatgcct ccatcaggag gcggtttgcg cgaaacagcg tctgacgcac
     3721 cagacgttcc ggcagggtgt cggcattaaa gctgtaccgc tgctccatct cattcatggt
     3781 tgtacgcagg tcgcacatgg cattggccac ctccgccact tcggcgcgca gtttttcgtg
     3841 tccggcatag ttcatttacg cagcctccgg cataaaccag tcatcatttg cggccccctg
     3901 ctgcatggcc cgctgtacgg cagccagacg ttgctgtccg gcacggtgat actgctcaag
     3961 cagctcgata ccgatatacc ggcgtccgga ctggagggcg gccacgcagg ttgagccgct
     4021 gcctgcaaac gggtccagca caatcgcgtt cggatgtgtg aagctctcaa tcagtggttg
     4081 caggctggta acgggctttt ccgtcgggtg atgacgattg cctgaatatt tccagcccag
     4141 cacatccggc agagggttac ggggccgggc cggactgccc tttgccagga tgtaagcgca
     4201 ttcgtggcgg tacgcgacat acgcagattt ggatgtgtag tttttggtga acacgaggtg
     4261 ccctgccacg ctgaatccgg cgcgtttcca ggccgccata aagcggtcaa tacggtgcca
     4321 gccgtagaag ctgaccatca gcgcgtcttt tttgagtacg cggtacattt cattacaggc
     4381 cggttgcagc cattcatcgg ttttatcgcc agcgatggtg cgcccgaaac ggtcacgaaa
     4441 accgacgagg tacggcgggt cagtcagtat gaaatcaatg gcgttatcag gaatacgggc
     4501 cataacatcg atgcagttac cgaggacaaa acgggacata acaacgctcc ttctcttaca
     4561 gaggacaccg ggagggcacc cctgtggtgc ccgttccccg ctgcctgtgg gctgttcacc
     4621 gcgtccggta acgggtcagg ccgaccgtgg aataccgcag ccgtcgtctt tctgacggca
     4681 aggctatgcc gcgaaagcgg cttgaagggc gttcatcaga aaagggcgac aggctggcgt
     4741 acactgtcac cggtgccagc cgggagcggt aaaagcgccc gtcccgtgcc cggccggacg
     4801 cgggacactg accacaggca gcgtttatgc cgcgccgcgt cagccggcgc agtaaaaaga
     4861 gaagcacagc gacatctttt gttactcgcc aaacaaaacc caaaaacaac ccacaaacac
     4921 cccaataaac accaaaaaca atacaagtaa tcattgattg atagttgaaa tggggtaaac
     4981 ttgacaaaca accccattta aaacccaaag cacacccaaa aacacaccac aaaaacacca
     5041 taaggagttt tataaatgtt ggtattcatt gatgacggtt caacaaacat caaactacag
     5101 tggcaggaaa acgacggaac aattaaacag cacattagcc cgaacagctt caaacgcgag
     5161 tgggcagtct cttttggtga taaaaaggtc tttaactaca cactgaacgg cgagcagtat
     5221 tcatttgatc caatcagccc ggatgctgta gtcacaacca atatcgcatg gcaatacagc
     5281 gacgttaatg tcgttgcggt gcaccacgcc ttactgacca gtgatctacc ggtaagcgaa
     5341 gtggatattg tttgcacact tcctctgaca gagtattacg acaggaataa ccaacccaat
     5401 acggaaaata ttgagcgtaa aaaagcaaat ttccggaaaa aaattacatt aaatggtggg
     5461 gatacattca caataaaaga tgtaaaagtc atgcctgaat ctataccggc aggttatgaa
     5521 gttctacaaa aactggatga gttagattct ttattaatta tagatctcgg ggggaccaca
     5581 ttagatattt ctcaggtaat gggtaaatta tcagggatca gcaaaatata cggagactca
     5641 tctcttggtg tatctctggt cacatctgca gtaaaagatg ccctttctct tgcgagaaca
     5701 aaa
//
LOCUS       DAZUCC010000259         5675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZUCC010000259 DAZUCC010000000
VERSION     DAZUCC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.260"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..176)
                     /locus_tag="ACHY09_004542"
     CDS             complement(<1..176)
                     /locus_tag="ACHY09_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFS3887413.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLE"
     gene            complement(280..1017)
                     /gene="bamD"
                     /locus_tag="ACHY09_004543"
     CDS             complement(280..1017)
                     /gene="bamD"
                     /locus_tag="ACHY09_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFS3887414.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQLNAQAEKVAKIIAANSSNT"
     gene            1152..2132
                     /gene="rluD"
                     /locus_tag="ACHY09_004544"
     CDS             1152..2132
                     /gene="rluD"
                     /locus_tag="ACHY09_004544"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFS3887415.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            2129..2860
                     /gene="yfiH"
                     /locus_tag="ACHY09_004545"
     CDS             2129..2860
                     /gene="yfiH"
                     /locus_tag="ACHY09_004545"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFS3887416.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYLLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2990..5563
                     /gene="clpB"
                     /locus_tag="ACHY09_004546"
     CDS             2990..5563
                     /gene="clpB"
                     /locus_tag="ACHY09_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS3887417.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1409 a         1351 c         1544 g         1371 t
ORIGIN      
        1 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
       61 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      121 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      181 gcgtttccgg attcttacac agccactgga tcggtcaact gatccttaac tgatcggcat
      241 tacagctcaa gggctgccgt tttgtgtttc aggtttctgt tatgtattgc tgctgtttgc
      301 ggcgatgatt ttcgctactt tttcagcttg cgcattcaac tgcatctgac ggtatgcatt
      361 ttccatcagc ggcagcgcat cacgcgtagc ctgggtatcc gggtagtcgc gcaacatgcc
      421 ttctacgcgg ttaacgacgg caacccatgc accacgttct gtatagtact cggcaacgga
      481 gtattcatat ttcgccagac gatctttcag gaataccaga cgtttggtgg catcggtggt
      541 gtactgactg ttcggatagc cgcgcaccag tttggaaaag tcactaaacg cagctcgtgc
      601 atgttgagga tcgcgatcgc tacggtcaac gccaaagaac ccttgcagcg cactgtcatc
      661 cagcgccata ttggtcaggc cacgcatgta catgacataa tcgatattcg gatgggtcgg
      721 gttaaggcga ataaaacgat cgatggcagc ctgtgctaac ggcaaatcgg cgtttttata
      781 gtaggcgtag atgagatcca gctgcacctg ctgcgaatac ggaccaaacg gatagcgatt
      841 atctaacgct tccagttgcg ttattgcctg tctccagtta ccgtcctgca gcttttgttg
      901 tgcagtcgcg taaatttcat ttggcggatt atcaggtact tcttcctttg accccgagca
      961 acccgccaaa aacaggctta gtgtggcggc tgccaccaga tatttcatgc gcgtcatgac
     1021 gttttgactt tcctcaaaat gtaatacggg agattctctg ttcctgctcc cggttaagac
     1081 cagctacaat agcacactat attaaacggc aaagccgtaa aaccccaacg ataaacgaag
     1141 aagcagtata tatggcacaa cgagtacagc tcactgcaac ggtgtccgaa aaccaactcg
     1201 gtcaacgctt agatcaggct ttggccgaaa tgttcccgga ttattcacgt tcgcgaataa
     1261 aagaatggat cctcgaccag cgcgtgctgg ttaacggcaa agtttgtgat aagccgaaag
     1321 aaaaagtatt gggtggcgag caggtggcca tcaacgctga gattgaagaa gaagcgcgtt
     1381 ttgaaccgca ggatatcccg ctggatatcg tctatgaaga tgaagatatc atcgtcatta
     1441 ataaaccacg cgacctggtg gtacatcctg gcgcgggtaa cccggatggt acggtactga
     1501 atgcgttgct tcattattat ccgcccattg ccgatgtacc gcgtgcgggc atcgtccatc
     1561 gtctggataa agacaccacc ggcctgatgg ttgtggcaaa aaccgttccg gctcagacgc
     1621 gtttagtcga atctttgcaa cggcgtgaaa ttactcgtga gtatgaagcg gtggcaattg
     1681 gtcatatgac cgctggcggt acagtagatg aaccaatcag ccgccacccg accaaacgta
     1741 ctcacatggc ggtgcatccg atgggtaaac cggcggtcac tcactatcgc atcatggaac
     1801 acttccgtgt gcacacgcgt ctgcggttgc gtctggaaac cggacgtacg caccagatcc
     1861 gcgtgcatat ggcccatatc actcatccgc tggtgggcga tccggtttat ggtggccgtc
     1921 cgcgtccgcc aaaaggtgct tcggaagcgt ttatctccac gctgcgtaag tttgaccgcc
     1981 aggcgcttca tgcaaccatg ctgcgtcttt atcacccgat ctccggtatc gaaatggaat
     2041 ggcatgcgcc tattccagaa gatatggtgg agctgattga ggtgatgcgc gccgatttcg
     2101 aagagcataa ggatgaagtg gactggttat gagtaagctg attgtcccgc agtggccgct
     2161 gccaaaaggt gttgcggcct gtagctccac tcgtatcggc ggcgtgagct tgcccccgta
     2221 tgattcgctc aacctcggtg ctcattgtgg cgataacccg gatcacgttg aagagaatcg
     2281 taagcgactt tttgctgcgg gcaatttgcc ttcaaaaccg gtctggcttg agcaggtaca
     2341 cggcaaagat gtgcttaagc ttactggcga accttatgcc tcaaaacgag cagatgcctc
     2401 ttatagcaac acgccgggca cggtttgcgc agtgatgact gccgactgcc tccccgtgct
     2461 gttttgcaat cgagcgggaa cggaagtcgc cgccgctcat gctggctggc gtggactgtg
     2521 ctcaggcgtg ctggaagaga cggtttcctg ttttgctgat aatccggaaa atattctcgc
     2581 ctggttaggg ccggcaattg gtccacgcgc gtttgaagtg ggggcggagg ttcgcgaggc
     2641 gtttatggca gtagacgcca aagcgagtac agctttcatt cagcatggtg ataagtatct
     2701 ggcggatatt tatctccttg cccggcagcg tctggcgaac gtcggtgtcg aacaaatttt
     2761 cggcggtgac cgttgtacat atacggaaaa tgagactttc ttctcttatc gtcgcgacaa
     2821 gaccaccggt cgtatggcaa gtttcatttg gctgatataa cctaaagaat caagacgatc
     2881 cggtacgcgt gattttcttt tcacattaat ctggtcaata accttgaata attgagggat
     2941 gacctcattt aatctccagt agcaactttg atccgttatg ggaggagtta tgcgtctgga
     3001 tcgtcttact aataaattcc agcttgctct tgccgatgcc caatcacttg cactcgggca
     3061 cgacaaccaa ttcatcgaac cacttcattt aatgagcgcc ctgctgaatc aggaaggggg
     3121 ttcggttagt cctttattaa catccgctgg cattaatgct ggccagttgc gcacagatat
     3181 caatcaggca ttaaatcgtt taccacaggt tgaaggtact ggtggtgatg tccagccatc
     3241 acaggatctg gtgcgcgttc ttaatctttg cgacaagctg gcgcaaaaac gtggtgataa
     3301 ctttatctcg tcagaactgt tcgttctggc ggcacttgag tctcgcggca cgctggccga
     3361 catcctgaaa gcagcagggg cgaccaccgc caacattact caagcgattg aacaaatgcg
     3421 tggaggtgaa agcgtgaacg atcaaggtgc tgaagaccaa cgtcaggctt tgaaaaaata
     3481 taccatcgac cttaccgaac gagccgaaca gggcaaactc gatccggtga ttggtcgtga
     3541 tgaagaaatt cgccgtacca ttcaggtgct gcaacgtcgt actaaaaata acccggtact
     3601 gattggtgaa cccggtgtcg gtaaaactgc catcgttgaa ggtctggcgc agcgtattat
     3661 caacggcgaa gtgccggaag ggttgaaagg ccgccgcgta ctggcgctgg atatgggcgc
     3721 gctggtggct ggggcgaaat atcgcggtga gtttgaagaa cgtttaaaag gcgtgcttaa
     3781 cgatcttgcc aaacaggaag gcaacgtcat cctgtttatc gacgaattac ataccatggt
     3841 cggtgcgggt aaagccgatg gcgcaatgga cgccggaaac atgctgaaac cggcgctggc
     3901 gcgtggtgaa ttgcactgcg taggtgccac gacgcttgac gaatatcgcc agtacatcga
     3961 aaaagatgct gcgctggagc gtcgtttcca gaaagtgttt gttgccgagc cttctgttga
     4021 agacaccatt gctattctgc gtggcttaaa agaacgttac gaactgcacc accatgtgca
     4081 aattactgac ccggcaattg ttgcagcggc gacgttgtct catcgctaca ttgctgaccg
     4141 tcagctgcca gataaagcca tcgacctgat cgatgaggca gcatccagca ttcgtatgca
     4201 gattgactca aaaccagaag aactcgaccg actcgatcgc cgtatcattc agctcaaact
     4261 ggaacaacag gcgttaatga aagagtctga tgaagccagt aaaaaacgcc tggatatgct
     4321 caacgaagaa ctgagcgaca aagaacgtca gtactccgag ttagaagaag agtggaaagc
     4381 agagaaagca tcgctttctg gtacacagac cattaaagcg gaactggaac aggcgaaaat
     4441 cgctattgaa caggctcgcc gtgtggggga cctggcgcgg atgtctgaac tgcaatacgg
     4501 caaaatcccg gaactggaaa agcaactgga agccgcaacg cagctcgaag gcaaaactat
     4561 gcgtctgttg cgtaataaag tgaccgatgc cgaaattgct gaagtgctgg cgcgttggac
     4621 ggggattccg gtttctcgca tgatggaaag cgagcgcgaa aaactgctgc gtatggagca
     4681 agaactgcac catcgcgtta ttggtcagaa cgaagcggtt gatgcggtat ctaacgctat
     4741 tcgtcgtagc cgtgcggggc tggcggatcc aaatcgcccg attggttcat tcctgttcct
     4801 cggcccaacc ggtgtgggga aaactgagct ttgtaaggcg ctagcgaact ttatgtttga
     4861 tagcgacgag gcgatggtcc gtatcgatat gtccgagttt atggagaaac actcggtgtc
     4921 tcgtctggtt ggtgcgcctc cgggatatgt cggttatgaa gaaggtggct acctgaccga
     4981 agctgtgcgt cgtcgtccgt attccgtcat cctgctggat gaagtggaaa aagcgcatcc
     5041 ggatgtcttc aacattctgt tgcaggtact ggatgatggg cgtctgactg acgggcaggg
     5101 gagaacggtc gacttccgta atacggttgt cattatgacc tctaacctcg gttccgattt
     5161 gattcaggaa cgcttcggtg aactggatta tgcgcacatg aaagagctgg tgctcggtgt
     5221 ggtaagccat aacttccgtc cggaattcat taaccgtatc gatgaagtgg tggtcttcca
     5281 tccgctgggt gaacagcaca ttgcgtcgat tgcgcagatt cagttgaaac gtctgtacaa
     5341 acgtctggaa gaacgtggtt atgaaatcca catttctgac gaggcgctga aactgctgag
     5401 cgagaacggt tacgatccgg tctatggtgc acgtccgctg aaacgtgcta tccagcagca
     5461 gatcgaaaac ccgctggcac agcaaatact gtctggtgaa ttggttccgg gtaaagttat
     5521 tcgcctggaa gttaatgaag accggattgt cgccgtccag taatgataaa acgagccctt
     5581 cggggctcgt ttttgtctat aaattagacg gaaaagacta tgtttaagat gttttgcctg
     5641 aaaaatgagc gaacgataaa gtttttatat ttttt
//
LOCUS       DAZUCC010000260         5656 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZUCC010000260 DAZUCC010000000
VERSION     DAZUCC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.261"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..252
                     /locus_tag="ACHY09_004547"
     CDS             <1..252
                     /locus_tag="ACHY09_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887418.1"
                     /translation="SALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGFAL
                     GYYCQWRSKNRSQGQKWVEWGTCLSLAILAQGPLFNYLQ"
     gene            complement(306..1811)
                     /gene="glpD"
                     /locus_tag="ACHY09_004548"
     CDS             complement(306..1811)
                     /gene="glpD"
                     /locus_tag="ACHY09_004548"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFS3887419.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNAEQQSRVSQWLVEYTQQKLSLAS"
     gene            2001..2327
                     /gene="glpE"
                     /locus_tag="ACHY09_004549"
     CDS             2001..2327
                     /gene="glpE"
                     /locus_tag="ACHY09_004549"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFS3887420.1"
                     /translation="MEQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWRRQFPA
                     EVAYGA"
     gene            2372..3202
                     /gene="glpG"
                     /locus_tag="ACHY09_004550"
     CDS             2372..3202
                     /gene="glpG"
                     /locus_tag="ACHY09_004550"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFS3887421.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3219..3977
                     /locus_tag="ACHY09_004551"
     CDS             3219..3977
                     /locus_tag="ACHY09_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFS3887422.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(3959..5557)
                     /gene="rtcR"
                     /locus_tag="ACHY09_004552"
     CDS             complement(3959..5557)
                     /gene="rtcR"
                     /locus_tag="ACHY09_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFS3887423.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRL
                     ELLHDARSRSLFETLKCDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLSQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITREVVEDEINRLRYNWQES
                     RPSAITALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1269 a         1496 c         1523 g         1368 t
ORIGIN      
        1 tcagcgttgc tggaaaatat ccgaaacttc tttctgacgt ttgtctggct tcccctgaat
       61 ttttcccctt tttggccgca gccgtggact gattttgtcg gcccgattag tgcacagctt
      121 ggttttgcgt tgggatatta ctgccagtgg cgtagcaaaa atagaagcca ggggcagaag
      181 tgggttgagt ggggaacgtg cttaagtttg gcgattttag ctcaggggcc gttattcaat
      241 tatttacaat gaatagtcta aggaatccaa atagcccggt tgcacttcac aaccgggcat
      301 catccttacg acgccagcga caacttctgc tgcgtatact ccaccagcca ctgactcaca
      361 cgagattgtt gttccgcatt cagccacatg ccttgttttg tgcgacgcca cagggcatcg
      421 tcggcgcggc ggacccattc gtgatccacc aggtatttca gctccgcttc gtagaactca
      481 tgaccgaaat cttccccgag atcgcttacc gtccccgcat tgccgagcag caactcgctg
      541 ttgctgccgt acgtgcgcgc gtaatggcgt gccagcgatt cagtcaggaa cggatagcgg
      601 cggcgcaggc gagcggcata atcgtcgcgg tcgccttcaa tggcaccacc cggcagcacg
      661 ctctctttcg tccacgccgg accaatgccc tgataatatg gcgtcagttt ttccagcgcg
      721 tgctccgcca gtttgcggta ggtggtcagc ttaccgccga ataccgacag cagcggcgct
      781 ttgccgtttt catcatggat atcaagggtg taatcacgag tgatagcctg cggcgaatcg
      841 gactcatcat cacacagcgg acgcacgccg gagtaagtcc agacgatatc gtcccggctt
      901 aactgctttt taaagtgcgt gttatacact ttcagcaggt aattgatttc actctcttca
      961 atcttcactg cttttggatc acctttgtat tccacatcgg tggtgccgat gatggaaaac
     1021 tcgtccatcc acgggatcac gaagacaata cgtttatctt cgttttgcag aatgtaggcc
     1081 tgcttttgtg tatgaacgcg cggcacgaca atatggctgc ctttgatcag gcgaatgcca
     1141 taaggcgaag gcagatgcat cccgtcgtcg aagaactgct ttacccacgg gccggtggcg
     1201 ttcacgaggc cgcgcgcttg ccagctatat tttttgccgg tatcgatatc ttccgcttcc
     1261 acaatccaca ggccgttttc gcggcgagca gaagtggcac gagtacgagt aagcacttca
     1321 ccgcctttac gcactaccat ctgggcgttg gcgagtacca gacgggcgtc gtctacccaa
     1381 cagtcggaat attcgaatcc gcgcttaatt tccggtttta acactgaatt tgcgccaaaa
     1441 cgcaaaccgg ttgatcccgg caggctggtg cgtttaccca gatgatcgta cataaacaga
     1501 ccaatgcgaa tcatccacgc cgggcgcaga tgcggacgat gcggcaggcg aaaacgcatc
     1561 gggaaggcga tatgcggggc cattttcagt agcacttcac gttcagccag cgcctcgctg
     1621 accaggcgga attcatagtg ctcaaggtag cgcaggccac cgtgaatgag ttttgaactg
     1681 gcggaagagg tcgcgcaagc gagatcctgc gcctccagca tcagcacgga taaaccgcgt
     1741 ccagcggcgt ctgccgcgat accagcacca ttgatgccgc cacctatcac aatcagatct
     1801 ttggtttcca tgctgccctc attcactttc gttaaagctc ataaatgttc gttatcgaac
     1861 atattagcaa agaatcgcgc tttaggtaac attgaaaaaa cattttagag tgatatgtat
     1921 aacattatgg cgtttatctg ccgcttcgac gtaaactgtg cggtaaattt gcccacttgt
     1981 ttgtaaagaa agagagacgc atggagcagt tcgaatgtat taacgttgcc gtcgcgcacc
     2041 agaagttgca ggaaaaagag gcggtgctgg tcgatattcg cgatccacag agtttcgcaa
     2101 tggggcatgc ggtgcaggct ttccatttaa ctaacgacac actgggcgcg tttatgcgtg
     2161 ataacgactt tgacacaccg gtgatggtga tgtgttatca cggcaatagc agcaaaggcg
     2221 cggcgcagta tctgctgcaa cagggctacg atgtggtcta tagcattgac ggcggctttg
     2281 aagcctggcg gcgtcagttt cctgcagagg tggcgtacgg cgcgtaacgc tttatactgt
     2341 ccccttttgt gtggaataag cgacagcaac gatgttgatg attacctctt ttgctaaccc
     2401 ccgcgtggcg caggcgtttg ttgattacat ggcgacgcag ggtgttatcc tcacgattca
     2461 acaacataac caaagcgatg tctggctggc ggatgagtcc caggccgagc gcgtgcgggc
     2521 agagctggcg cgttttctcg aaaacccggc agatccgcgt tatctggcgg ccagctggca
     2581 ggcgggccat accggcagtg gcctgcatta tcgccgttat cctttctttg ctgccttgcg
     2641 tgaacgcgca ggtccggtaa cctgggtgat gatgatcgcc tgcgtggtgg tgtttattgc
     2701 catgcaaatt ctcggcgatc aggaagtgat gttatggctg gcctggccat tcgatccaac
     2761 actgaaattt gagttctggc gttacttcac ccacgcgtta atgcacttct cgctgatgca
     2821 tatcctcttt aacctgctct ggtggtggta tctcggcggt gcggtggaaa aacgcctcgg
     2881 tagcggtaag ctaattgtca ttactctcat tagcgccctg ttaagcggct atgtgcagca
     2941 aaaattcagc gggccgtggt ttggcgggct ttctggcgtg gtgtatgcgc tgatgggcta
     3001 cgtctggcta cgtggcgaac gcgatccgca aagtggcatt tacctgcaac gtgggttaat
     3061 tatctttgcg ctgatctgga ttgtcgccgg atggtttgat ttgtttggga tgtcgatggc
     3121 gaacggagca cacatcgccg ggttagccgt gggtttagcg atggcttttg ttgattcgct
     3181 caatgcgcga aaacgaaaat aattccaggg atttataaat gaaacaaaca caacgtcaca
     3241 acggtattat cgaactggtt aaacagcagg gttatgtcag taccgaggaa ctggtggagc
     3301 acttctccgt cagcccgcag actattcgtc gcgacctcaa cgagctggca gagcaaaacc
     3361 tgatcctgcg ccatcatggc ggtgcggcgc tgccttccag ttcggttaac acgccatggc
     3421 acgatcgtaa agccacccag accgaagaaa aagagcgcat cgcccgcaaa gtggcggagc
     3481 aaattcctaa tggctcaacg ctgtttatcg atatcggcac caccccggaa gcggtggcgc
     3541 acgcgctgct caatcacagc aatttgcgca ttgtcaccaa caatcttaac gtcgctaaca
     3601 cgctgatggt aaaagaggat tttcgcatta ttctcgccgg tggcgaatta cgtagccgcg
     3661 atggtgggat cataggcgaa gcgacgctcg attttatctc ccagttccgc cttgatttcg
     3721 gcattctggg gatcagcggc atcgatagcg acggctcgtt gctggaattc gattaccacg
     3781 aagttcgcac caaacgcgcc attattgaga actcgcgcca cgttatgctg gttgtcgatc
     3841 actcgaaatt tggccgtaat gcgatggtca atatggggag catcagcatg gttgatgctg
     3901 tctacaccga cgccccgccg ccagtaagcg tcatgcaggt gctgacggac catcatattc
     3961 aactggagct gtgctgatcc tgcacggctt cccacgtcag accaaaacgc gccaggtatt
     4021 tgcgtagccg atctgcgtcg ttgacgctgg ctttgccctg gcgcgaaaca tcaaaaagct
     4081 ggcgaccggc agcagaaagt gactttgcct ggcggcagat ggctattacg tgttccagtt
     4141 gcatacggtc gaagagatcg atgttttctg cctctgcgcc cagcaacgcc gtaatcgcgg
     4201 aggggcgact ctcctgccag ttatagcgca gacggtttat ctcatcttcc accacttccc
     4261 gagtgatgcg tccgctagtg gcaaaggttg ccatccgcgt gacgctggca gaaagttcgc
     4321 gaaagttacc gcgccatgtt gcctggggag aggtcgcaaa agccagccag gcgcgccgcg
     4381 cttcggtgtt aaaacgcacg ctgtcgccag tgagtgaggc gtggcgctcc acttcataat
     4441 caaggttcgg ttcaatatct tcctggcgct ggctcagccc cggcaaggtg aaggtccaga
     4501 gattgatccg cgcgtacaga tcttcgcgga atttgccttc ggcaaccagc tgacgcaaat
     4561 cgcgcaccgt tccggcgata agctgaaagt cgctactcac ctggcgatcg ctgccaaacg
     4621 gataaaaggt tttctcttca atggctttca gcagcattgc ctgttcgtct gcgcccagtt
     4681 cgccaatctc atcaagaaat aacatcccgc cgttggcgct gcgtaataat ccttcccgcg
     4741 actcccgcgc cccggtaaac gcgcctttca catgaccaaa tagcgtcgac atggcggtat
     4801 cgccacgcag agtggcgcag ttcatttcta caaatgcgcc gctaaactga tgccgtgcct
     4861 gttttaattc gaagatgcgt cgtgccagaa atgacttgcc cgcacctgtc gggccgttaa
     4921 gcaggatcgg tgcgcgggat ttaatcgcca ctttttcaat ctgctcaatc atgcggttga
     4981 agtgggggtt acgcgtggca atgccggact taagaaaatc gagcgtttgc tgccgttcct
     5041 cggcaaagcg gctggcgatg gcgttataac ggcttaaatc gagatcgata atcgtcactt
     5101 cacctgcgcc gcgcggctgt tcttttttgc gcggtgggga agattgtatc agccgggcgg
     5161 gcaggtaacg cgcttccgcc agcagaaacc agcaaatctg tgcgacgtgg gtgccagtgg
     5221 tgatgtgaat taaatagtct tctttatcag gttgaaacgc gtagccacgg gcgaaatcat
     5281 gcaggcaggc gtaaacctct tcgaaatccc acgggttatg cagttcaatc tcaacgctca
     5341 ccacttcagt ttctggcgaa acgctggcga tatcgcattt aagcgtttca aacagcgagc
     5401 gcgagcgggc gtcgtgcaac aattccagtc gattgatgac taacgattct tgctggcata
     5461 gacagagtga cggacgccat ttcgaccagc gctgactgcc gcgcccggca taatccagca
     5521 ccgttccgac aaagccaaaa gccactgttt tacgcatctt agatatcctt ataaaagacg
     5581 agatttcatt atcggtaaat cggctttgcg ggaaatctcc ggttacggac gtaaagatat
     5641 aatcttttta aaaaca
//
LOCUS       DAZUCC010000261         5648 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZUCC010000261 DAZUCC010000000
VERSION     DAZUCC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.262"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            123..1631
                     /gene="casA"
                     /locus_tag="ACHY09_004553"
     CDS             123..1631
                     /gene="casA"
                     /locus_tag="ACHY09_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFS3887424.1"
                     /translation="MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDME
                     LATLALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVCGIDLRSTVLLNVLTIPRLQKQFPNELHTENQPTWVKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANINFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
     gene            1624..2106
                     /gene="casB"
                     /locus_tag="ACHY09_004554"
                     /gene_synonym="cse2"
     CDS             1624..2106
                     /gene="casB"
                     /locus_tag="ACHY09_004554"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFS3887425.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QSFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKTEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            2119..3210
                     /gene="cas7e"
                     /locus_tag="ACHY09_004555"
     CDS             2119..3210
                     /gene="cas7e"
                     /locus_tag="ACHY09_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFS3887426.1"
                     /translation="MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSL
                     KHAMRKSDYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKLVDE
                     AEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
                     ALSGRMATSGMMSELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQE
                     FSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPA
                     DMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSL
                     SDVDPITAQVKQMPTLEQLKSWVRNNGEA"
     gene            3213..3887
                     /gene="cas5e"
                     /locus_tag="ACHY09_004556"
     CDS             3213..3887
                     /gene="cas5e"
                     /locus_tag="ACHY09_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFS3887427.1"
                     /translation="MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TIQTWREYLCDASFTVAIWLTPQATMVMSELEKAVLKPRYTPYLGRRSCPLTQPLFLG
                     TCQASDPQKALLNYEPVGGDIYSEESVDGHHLKFTVRDEPMITLPRQFASREWYVIKG
                     GMDVSQ"
     gene            3874..4473
                     /gene="cas6e"
                     /locus_tag="ACHY09_004557"
     CDS             3874..4473
                     /gene="cas6e"
                     /locus_tag="ACHY09_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFS3887428.1"
                     /translation="MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKR
                     NTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKR
                     LDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQT
                     VCFEGVITINDAPALIDLVQQGIGPAKSMGCGLLSLAPL"
     gene            4489..5406
                     /gene="cas1e"
                     /locus_tag="ACHY09_004558"
     CDS             4489..5406
                     /gene="cas1e"
                     /locus_tag="ACHY09_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFS3887429.1"
                     /translation="MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYVLLAKQYGVKW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRIPGEPDREVRLACRDIFRSSKTLVKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            5408..>5648
                     /gene="cas2e"
                     /locus_tag="ACHY09_004559"
     CDS             5408..>5648
                     /gene="cas2e"
                     /locus_tag="ACHY09_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFS3887430.1"
                     /translation="MSMLVVVTENVPSRLRGRLAIWLLEVRAGVYVGDVSARVREMIW
                     EQISGLAEEGNVVMAWATNTESGFEFQTFGVNRRTP"
BASE COUNT         1526 a         1154 c         1430 g         1538 t
ORIGIN      
        1 tttgcagtga atgtgattat tgaattaatt acgccgtatt ttttctttgt ttttaccgat
       61 aacggaagtg tgccgacgca tagaaatgca ggagaaatgt cggagcatat gaaggagaac
      121 aaatgaattt gcttattgat aactggatcc ctgtacgccc gcgaaacggg gggaaagtcc
      181 aaatcataaa tctgcaatcg ctatactgca gtagagatca gtggcgatta agtttgcccc
      241 gtgacgatat ggaactggcc actttagcac tgctggtttg cattgggcaa attatcgccc
      301 cggcaaaaga tgacgttgaa tttcgacatc gcataatgaa tccgctcact gaagatgagt
      361 ttcaacaact catcgcgccg tggatagata tgttctacct taatcacgca gaacatccct
      421 ttatgcagac caaaggtgtc aaagcaaatg atgtgactcc aatggaaaaa ctgttggctg
      481 gggtaagcgg cgcgacgaat tgtgcatttg tcaatcaacc ggggcagggt gaagcattat
      541 gtggtggatg cactgcgatt gcgttattca accaggcgaa tcaggcacct ggttttggtg
      601 gtggctttaa aagcggttta cgtggaggaa cacctataac caccttcgta tgtgggatcg
      661 atcttcgctc gacggtatta ctcaatgtcc tcacaatacc tcgtcttcaa aaacaatttc
      721 ccaatgaatt acatacggaa aaccaaccta cctgggttaa gcctgtcaag ccgaatgaat
      781 cggtgcctgc ttcttcaatt gggtttgtcc gtggtttatt ctggcaacca gcgcatattg
      841 aactatgcga tcccattggg attggtaaat gttcttgctg tggtcaggaa agcaatttgc
      901 gttataccgg ttttcttaag gaaaaattta cctttacagt taatgggcta tggccccatc
      961 cgcattcccc ttgtctggta acagtcaaga aaggggaggt tgaggaaaaa tttcttgctt
     1021 tcaccacctc cgcaccatca tggacacaaa tcagccgagt tgtggtagat aagattattc
     1081 aaaatgaaaa tggaaatcgc gtggcggcgg ttgtgaatca attcagaaat attgcgccgc
     1141 aaagtcctct tgaattgatt atggggggat atcgtaataa tcaagcatct attcttgaac
     1201 ggcgtcatga tgtgttgatg tttaatcagg ggtggcaaca atacggcaat gtgataaacg
     1261 aaatagtgac tgttggtttg ggatataaaa cagccttacg caaggcgtta tatacctttg
     1321 cagaagggtt taaaaataaa gacttcaaag gggccggagt ttctgttcat gagactgcag
     1381 aaaggcattt ctatcgacag agtgaattat taattcccga tgtattggcg aatattaatt
     1441 tttcccaagc tgatgaggta atagctgatt tacgagacaa acttcatcaa ttgtgtgaaa
     1501 tgctatttaa tcaatctgta gctccctatg cacatcatcc taaattaata agcacattag
     1561 cgcttgcccg cgccacgcta tacaaacatt tacgggagtt aaaacctcaa ggagggccat
     1621 caaatggctg atgaaattga tgcaatggct ttatatcgag cctggcaaca actggataat
     1681 ggagcatgtg cgcaaattag acgtgtttca gaacctgatg aattacgcga tatccctgcg
     1741 ttttataggc tggtgcaatc ttttggttgg gaaaacccac gtcaccagca ggctcttttg
     1801 cgcatggtgt tttgcctgag cgcaggaaag aatgtcatcc gacatcagga caaaaaaacg
     1861 gagcaaacaa caggtatctc gttgggaaga gctttagcca atagtggaag aattaacgag
     1921 cgccgtatct ttcaattaat tcgggctgac agaacagccg atatggtcca gttacgtcga
     1981 ttacttactc acgccgaacc cgtacttgac tggccattaa tggccaggat gttgacctgg
     2041 tggggaaagc gcgaacgcca gcaacttctg gaagattttg tattgaccac aaacaaaaat
     2101 gcgtaaggaa acctttctat gtctaacttt atcaatattc atgttctgat ctctcacagc
     2161 ccttcatgtc tgaaccgcga cgatatgaac atgcagaaag acgctatttt cggcgggaaa
     2221 agacgagtaa gaatttcaag tcaaagcctt aaacatgcga tgcgtaaaag tgattattac
     2281 gcacaaaata ttggtgaatc cagtctcaga acaattcatc ttgcacaatt acgtgatgtt
     2341 cttcggcaaa aacttggtga acgttttgac caaaaaatca tcgataagac attagcgctg
     2401 ctctccggta aattagttga tgaagccgaa aagatttctg ccgatgcggt tactccctgg
     2461 gttgtgggag aaatagcctg gttctgtgag caggttgcaa aagcagaggc tgataatctg
     2521 gatgataaaa agctgctcaa agttcttaag gaagatattg ccgccatacg tgtgaattta
     2581 cagcagggtg ttgatattgc gcttagtgga agaatggcaa ccagcggcat gatgagtgag
     2641 ttgggaaaag ttgatggtgc aatgtccatt gcgcatgcga tcactactca tcaggttgat
     2701 tctgatattg actggttcac cgctgtagat gatttacagg aacaaggttc tgcacatctg
     2761 ggaactcagg aattttcatc gggtgttttt tatcgttatg ccaacattaa cctcgctcaa
     2821 cttcaggaaa atttaggtgg tgcctccagg gagcaggctc tggaaattgc aacccatgtt
     2881 gttcatatgc tggcaacaga ggtccctgga gcaaaacagc gtacttatgc cgcttttaac
     2941 cctgcggata tggtaatggt taatttctcc gatatgccac tttctatggc aaatgctttt
     3001 gaaaaagcgg ttaaagcgaa agatggcttt ttgcaaccgt ctatacaggc gtttaatcaa
     3061 tattgggatc gcgttgccaa tggatatggt ctgaacggag ctgctgcgca attcagctta
     3121 tctgatgtag acccaattac tgctcaagtt aaacaaatgc ctactttaga acagttaaaa
     3181 tcctgggttc gtaataatgg cgaggcgtga acatgagatc ttatttgatc ttgcggcttg
     3241 ctgggccaat gcaagcctgg gggcagccga cctttgaagg aacgcgacct accggaagat
     3301 ttccgacccg aagcgggtta ttagggctac tcggggcttg tcttgggatc caacgtgatg
     3361 atacttcttc attacaggcg ttatcagaga gtgtgcaatt tgcagtgcgc tgcgatgaac
     3421 tcattcttga cgatcgtcgt gtgtctgtaa cggggttgcg tgattaccat acagtccttg
     3481 gagcgcgaga agattaccgt ggtttgaaaa gtcatgaaac gattcaaaca tggcgcgaat
     3541 atttatgtga tgcctccttt accgtcgcta tctggttaac accacaggca acgatggtta
     3601 tgtcagaact tgaaaaagca gtattaaagc ctcggtatac accgtatctg gggcggagga
     3661 gttgtccgct aacacaaccg ctttttctcg ggacatgtca ggcgtcggat cctcagaagg
     3721 cgctattaaa ttatgagccc gttggcggcg atatttatag tgaggaatca gttgacgggc
     3781 atcatttaaa atttacggtg cgcgacgaac cgatgatcac cttacctcga caatttgctt
     3841 cccgagaatg gtatgtgatt aaaggaggta tggatgtatc tcagtaaagt catcattgcc
     3901 agggcctgga gcagggatct ttaccaactt caccagggat tatggcattt atttccaaac
     3961 agaccggatg ctgctcgtga ttttcttttt catgttgaga agcgaaacac accagaaggc
     4021 tgtcatgttt tattgcagtc agcgcaaatg cctgtttcta ctgccgttgc gacagtcatt
     4081 aaaactaaac aggttgaatt tcaacttcag gttggtgttc cactctattt tcggcttcgg
     4141 gcaaatccga tcaaaactat tctcgacaat caaaagcgcc tggacagtaa agggaatatt
     4201 aaacgctgtc gggttccgtt aataaaagaa gcagaacaaa tcgcgtggtt gcaacgtaaa
     4261 ttgggcaatg cggcgcgcgt tgaagatgtg catcccatat cggaacggcc acagtatttt
     4321 tctggtgatg gtaaaagtgg aaagatccaa acggtttgct ttgaaggggt gatcaccatc
     4381 aacgacgcgc cagcgttaat agatcttgta cagcaaggta ttgggccagc taaatcgatg
     4441 ggatgtggct tgctatcttt ggctccactg tgattgaggt gtaataaaat gacctggctt
     4501 ccccttaatc ccattccact caaagatcgc gtctccatga tctttctgca atatgggcag
     4561 atcgatgtaa tagatggcgc gtttgtactt atcgacaaga cagggatccg cactcatatt
     4621 cctgttggct cggttgcctg catcatgttg gaacctggta cacgggtttc gcatgcagct
     4681 gtacgcctgg ctgcgcaagt tggaacatta ttggtatggg tgggggaagc gggcgttcgt
     4741 gtttatgctt ctggtcagcc tggtggtgcg cgttcagata agctgctcta tcaggcaaaa
     4801 cttgctctgg atgaagattt gcgtctgaag gtcgtacgta aaatgtttga acttcggttt
     4861 ggagaacctg cgcctgcccg gcgctccgta gagcaactca gaggtataga aggaagtcgt
     4921 gtgcgagcaa cctatgtact tctggcgaag caatacgggg tgaaatggaa tggacgtcgt
     4981 tacgatccga aagactggga aaagggcgat acggtcaacc aatgcattag cgctgcaact
     5041 tcttgtttat atggcgtaac tgaagcggcg atacttgcag ctggttatgc accagctatt
     5101 gggtttgtgc atacaggaaa gcctctttcc tttgtttacg atatcgcgga catcattaaa
     5161 tttgacactg ttgtaccgaa agcttttgag atagcgcgtc gtattcctgg tgagccggac
     5221 cgggaagtcc gtttggcgtg cagggatatt tttcgcagta gtaaaacatt agtcaaattg
     5281 attccgctta tagaggacgt gcttgctgct ggggaaatac agccgccggc cccacctgaa
     5341 gatgcgcagc ctgttgctat tccacttccc gtttctctgg gagatgccgg acatcggagt
     5401 agctgagatg agtatgttgg tcgtggtcac tgaaaatgta ccttcgcgct tacgaggcag
     5461 attagccatc tggttgttgg aggtacgtgc aggggtgtat gtaggtgatg tatccgcaag
     5521 agtccgtgaa atgatctggg aacaaatatc tggactggcg gaagaaggca atgtggtgat
     5581 ggcatgggct acgaatacgg aatcgggatt tgagttccag acatttgggg taaacaggcg
     5641 tactccgg
//
LOCUS       DAZUCC010000262         5645 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZUCC010000262 DAZUCC010000000
VERSION     DAZUCC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.263"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(256..4830)
                     /gene="ypjA"
                     /locus_tag="ACHY09_004560"
     CDS             complement(256..4830)
                     /gene="ypjA"
                     /locus_tag="ACHY09_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFS3887431.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQKVDERGTTNNTHIINHGQQNVYGGISNGSLIESGGYQDVGGHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLDSKSTAKNTQIYSGGTQIVDYTSTSDVIEIYSGGVLDVRGGM
                     ATNVTQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVMAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATDANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLTNGGVLTVESDTTSAKTQVNA
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSIAGYLATNVLLENGGNLLV
                     LAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGRSKDEFQVLARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     SELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPKPDPKPDPKPDP
                     KPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNN
                     VWGTTYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHTHIFFD
                     RGGHGSVGSYSLGGYASWEHESGFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSS
                     SLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
     gene            complement(4895..5014)
                     /locus_tag="ACHY09_004561"
     CDS             complement(4895..5014)
                     /locus_tag="ACHY09_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFS3887432.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(5169..5435)
                     /locus_tag="ACHY09_004562"
     CDS             complement(5169..5435)
                     /locus_tag="ACHY09_004562"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS3887433.1"
                     /translation="MLRVTTSAHNRGSRPGQNKRLRTLSVGDALLVCKLNRLGRSMWH
                     LVVLLEELCERGINFRALAQSIFAQQWGDGSCKSKTICDLRVIV"
BASE COUNT         1331 a         1514 c         1220 g         1580 t
ORIGIN      
        1 atacataaaa acatttagaa ataatctgaa attttttgtt tttcatattg ttatgcaaat
       61 tcggtgtttg ggttcaactc ccgccagctc caccaatcat gattggacgg tgtaaggaca
      121 acaccaacaa aaacaggaag ttagcagtct cagcaagaca ccgaccagac ggtgaggaga
      181 cataaaagga tacgcaaagg agccgcggct cctggtgata tgaaagccca cagatgtggg
      241 ctttttcgtt gatggtcaga acgaccagtt cacaccagcc accgcgttcc acggggattc
      301 cacaccggca ccatggctat accccacccc gagatgcccg cttaacgaac tgctgaatga
      361 ggctttaata cctgcctggt atattccacg tctgcccgac aaatcattga caaaattacc
      421 gtcattattc actttcaccc ggttatcatc gacaaattct ttgcgcacag ccgccttcag
      481 ccacggctca acttccatgc cgttccccag acgcatgttg taactcagcg ttgcgcccag
      541 ttcacgatat atactacggg tatcgactga tttcgattcc atgccattgg ataaatgata
      601 ttcggggtta tcagcggtga accccgttaa cgatgcatac ggcgtcaggt tccagttacc
      661 atcggtaaat cgcatcccgg tttcaatgtg accgcccagc ccgttgctgc gataactgcc
      721 gttggcggct ccaccgctgc tcattttacc ggctacgtta ctttcaaagc ggttcagctt
      781 cacgataccg tccagataga aaccactttc atgttcccag ctggcatagc cccccagaga
      841 ataactgccc acactgccat gccctccacg atcaaaaaag atatgtgtat gggaataacc
      901 cataaaagcg cccagcgtcg caatcccctc aggaatatca ttacggctat cgatccccac
      961 cgtcattccg gtcagcgtct gctcaaaccc ggcacccgca tcggtggtga cattattacg
     1021 ggtgttatac gtcgtccccc agacattatt gttgtgtgga ctcgctttca ttatgttcaa
     1081 ccgctcgcga atactgttta gctcagcatc aaagaccaac ggtaatgttg ctgccatatt
     1141 gagtacggct gccgtagaag gcgtgatgcg tttctccggg acgggtgtcg gcgttggatc
     1201 gggagtcggg tctggtttcg gatccggttt tgggtctggt tttggatccg gcttcggatt
     1261 tgggttgggg tccgggttgg gtttgacatc attggtcagg ttccagttgc tgttgccatc
     1321 gcttttcagg acatactcat aggtcccaag atcaacgaaa ccgccggtat tacccagcgt
     1381 aaacgaagca tcccctcccc ctgttttcac cagcgtcatc gcgtcgtcag actgagggct
     1441 gacgccggta tcctgaacaa agattttaaa attgccagtg gcgttgttgt tgacgaccag
     1501 ttgatcgccc cgggagcctg caacgttggt atgcaggtag aaattaccgc tgccggaaag
     1561 ttcactggtg gtcagcgtat tgtagatacc attagttgtg gcaggcgctg ctgtttgtgc
     1621 tgataaataa acatcaccgt cgttgaggag caaactattt actctatatt cgcagttgct
     1681 ggtgcctgca caggaattat tgctgttaag ccagacactg ccccggctgg cagccgtcag
     1741 gtcggcaagc gtcgtatcga caccatttcc cagagttaat gtcgcgctat cggtaatccg
     1801 gaccgcccct tcaagtttta ctggcgtaac attatcccgt ggcgtcatta acgacaggct
     1861 tcctgtcgcg ccactgaccg atgcatccgc cagcgtacct gcgtagacga ttgccgtccc
     1921 gccagcaacc tgtagatctt ctgcccgggc cagaacctga aactcatctt ttgaccgacc
     1981 gagggtatat tgcccaccag aattaacctt tgtggcgaag tcctgcccca ggttttgcat
     2041 tgccccaccg ttgccaaccg tggaatcgcg agcttcggta cctgctaata ccaataaatt
     2101 accgccattt tccagcagta cattggtcgc taaatatccg gcaatggaaa aggtgccgta
     2161 ctggtgcgta ccgctgattt caataccgtt agccgtgctc gtctggagag cggcaccgct
     2221 gttctggacg atatctgtcg ctttgccatt atcgttgact gtcagcgttc cgccttcatt
     2281 gatcgttgtt tttattgcct ctccgttagc tgaaactgtt tgtattccgc cgtcgttaat
     2341 tgttgtctca tttgccacac cctcgacgat ttgttcaccg ccagtgagcg tcgtgccagt
     2401 cgcagtggct tttgttttga cgatctcccg accgcccgca ttgacctgtg ttttggcgga
     2461 agtggtgtct gactccacgg ttaacacgcc gccatttgtc agcaggatat tgttcgccgc
     2521 accctgctcg atgctgaacg cgacgccatc agcgcgtgtc cctgtgaccc gcgtcgccct
     2581 ggtggttgca accaacgcgc cctgactact ctgctgtatc cccgttgcgc tgcctttctc
     2641 ccgcacatcg agtgtgccgc cgtcattaag caccgagttt tcagccagac cgccctcatt
     2701 aactacctgt gaaccgccat taataatgga accttccgct gtcccgtttg ccataatttg
     2761 ttgtaagcca gagacgatat cggtattgat cgccttacca taattctgaa cggtttgtgt
     2821 gccgccattg atgtgcgttt tgtctgttga cccaccatca acaatttgtt caccactttc
     2881 aatatttgca tcagtggcta aaccatatac cgtttgcttg ccacctttga tatttgcttt
     2941 atcagaagtg gcactggcat atattgtttg ggtgcccgca ctattgagta cagtgccaac
     3001 atcttttcca taaacatcca ttttgccgtt ggcattaata atcgtatcaa ctgcccggga
     3061 accagtgacg actgttaatg agccagcgtt ttccagcact acatttttag cttctgaatt
     3121 cctgatgtag aaagcatcac cataactgtt ggttccttcg ataagggttc cggaagtcgt
     3181 ggaagcaatt aatgcgccgc cggattgttg ctcaacatgc ttagcctcac caccgtcctg
     3241 aacctccaga acgccaccat tattaagtct ggtgctatct gttttagcct ccttctggac
     3301 aatcagctta ccgccagcat caatggtagt atttttcgcc gaggttttag ccattaccgt
     3361 cagttcgccg gtattttcca gcacaacata attagcctca cctccggtaa tagtgaagtg
     3421 agagagctta ttgtatcctt cgatatcagt ccctgcgccc gtgttggcaa ctaaagcact
     3481 gcccgtctcc tggttaaccc catgtgcaat accgccggta tagacaatca gcgagcctcc
     3541 ggcgctaata ttgctgccag ttgccgtacc atctttctca acaacctgcc ggctcccaga
     3601 ggatatattt gtcgtgtcag ctttcccgcc gctcttaata ttttgtgttc cgctgttgat
     3661 attggtgccc gtggcaatac cataattatt aatattctgt gtgccaccat taattatggt
     3721 atttgtcgcg tttccggcaa catccataac gccgccatta tctattcggg tcgcatcagc
     3781 tttagcattc gttaaaactg acattgttcc tttatcttta ataatcgtct tatttgccga
     3841 accatatgcg tttatgtcta aatgaccacc gttttccagc aacacattgt ctgccacgtt
     3901 attgtggatg gagaatgccc cttcactatt cgtaccgctc accgtcaaat cgtaagtcgt
     3961 tactttcaaa gcggctccat cgtgctgagt aacatttgtt gccataccac ctctaacatc
     4021 aagcacgcca ccggaataaa tttcaataac atccgaggtg ctagtgtaat caacaatttg
     4081 cgtaccacca gaatagatct gagtattttt tgccgttgac ttactatcca gagactgagt
     4141 tccaccttca atcgtcgtgt ccagcgcacg gctctcatat actctttgct caccgccatt
     4201 tttaatggtg gttgtttcta ctgtgctctg ttcaacatac tgtcgaccac catttatggt
     4261 tgtgttcgtc gccagacttc cttgcgtgat gtcctgataa ccggacttat ttatcgttgt
     4321 accatcaaca tgcccctgag ttgttactgt ctgccctcca ccatcaatga tggttccatt
     4381 cgcagtcccc ccacttatgt gtgaattacc acccttaatt gtcgttccat tgctgatacc
     4441 acctgtataa acgtcctgat tgccactctc gattattgta cctgtggaaa tacccccgtc
     4501 atgaattgac tgtctgcccc cgttaatggt tgtattatta gcctgcccca caaaattgtt
     4561 atgacctcct acatcttgat atccacccga ttcaataaga cttccattag atatcccgcc
     4621 ataaacattc tgctggccat ggttgataat atgagtgtta tttgttgtac ctcgttcatc
     4681 tactttttgg ctgccatcta cagtctcatc gtttaccaca ccaataacat caggagtgaa
     4741 ggccgccatc ccgggcgggg catatatcaa ggcagatatc aataaggaaa gtactgagcg
     4801 acgacaataa tagggactgg tcctgttcat aaatttcatc ctctgaaaag tgaatactga
     4861 gtagcgttta agcgacctta gctttgctgc aacatcagcc cacaggcacc agaccagggg
     4921 attcatcctg aagagacagc gcaagtgtat tgtgttcacc gctcatcaaa gacatcatga
     4981 tgaaatgatg atattccgca taagaatgag gcatttttta aacgcagtgc gctgaagtgt
     5041 ggttggataa aaaagtcaat ctattcagga aacacgggcg tattcttttc tttcgacaat
     5101 caggcagtcg gaaaaataaa atgatttaca taatcgtttc tgatgaatat cttctgctca
     5161 cataaaaatc acacaataac tctgagatcg cagattgttt tacttttaca gcttccgtcc
     5221 ccccattgtt gggcaaatat agattgggcc agagcacgaa agttaatacc acgttcgcac
     5281 agctcctcca acagcacgac aagatgccac atactgcgcc ccagtcggtt cagtttacag
     5341 actagcagtg cgtcccctac cgataatgtc ctgagccgtt tgttctgtcc gggcctgctg
     5401 ccacggttat gcgctgaagt ggtaactctg agcacgcagt tcttcagtca ggcgtggtgc
     5461 accgtcaccc tattattgct gggtaagatc aaaaaacttt caagcagcta aggaaagttg
     5521 aaccagacat tagatgaaat atttcaacca attacagcac cagttcagac accgccaact
     5581 catcaaataa atcaaggggt tacgtgaaag cgtagcccct tttactttgg tagtgacagc
     5641 aaaat
//
LOCUS       DAZUCC010000263         5554 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZUCC010000263 DAZUCC010000000
VERSION     DAZUCC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.264"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..307
                     /gene="traQ"
                     /locus_tag="ACHY09_004563"
     CDS             17..307
                     /gene="traQ"
                     /locus_tag="ACHY09_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFS3887434.1"
                     /translation="MISKHRFSLPRLDITGMWVFSLGVWFHIVARLVYRKPWMAFFLA
                     ELIAAILVLFGAYQILDTWIARVSQEEREALEVRQQLLLRERKDNDDEHVSR"
     gene            294..839
                     /gene="trbB"
                     /locus_tag="ACHY09_004564"
     CDS             294..839
                     /gene="trbB"
                     /locus_tag="ACHY09_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFS3887435.1"
                     /translation="MFPVKSLSVVLLFLASCTQASTRAEIERLWHPEGMAARTTQPAA
                     DAQVRQEKAATRWFRLSNGTQVNLADWKVVLFMQGRCPYCHQFDPVLKQLALQYGFSV
                     FPYTLDGQGDTAFPQALPAPPDVLKTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            829..1115
                     /gene="trbJ"
                     /locus_tag="ACHY09_004565"
                     /pseudo
     CDS             829..1115
                     /gene="trbJ"
                     /locus_tag="ACHY09_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
     gene            1096..1488
                     /gene="trbF"
                     /locus_tag="ACHY09_004566"
     CDS             1096..1488
                     /gene="trbF"
                     /locus_tag="ACHY09_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFS3887436.1"
                     /translation="MKEKRNDAEQKNRKTKRDYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPKLLNFHYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRRDNKLREGGNNASH"
     gene            1475..2848
                     /gene="traH"
                     /locus_tag="ACHY09_004567"
     CDS             1475..2848
                     /gene="traH"
                     /locus_tag="ACHY09_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFS3887437.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFATNTTQ
                     AGVWQGQAAGYAYGGSLYARNQVRNIQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMANDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            2845..>5554
                     /gene="traG"
                     /locus_tag="ACHY09_004568"
     CDS             2845..>5554
                     /gene="traG"
                     /locus_tag="ACHY09_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFS3887438.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASSDPYTIIFSQPSPLRQVPNNNYSFLQSSEPFVSCK
                     DASVGLKDKLNFDTNTGGKTWNYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSAS
                     QIMRQNVTMNALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVLMRNLPM
                     VQTILVGITIGIFPLLVLAAVFNKLTLSVLKGYVFALMWFQTWPLLYAILNSAMTFYA
                     KQNGAPVVLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVRGLGAGFSSVYSHFASS
                     SITPTASAAGSVVDGNYSYGNMQTNNVNGFSWSTNSTTSFGQMTHQTGSGATATQTRD
                     GSMVMDASGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLS
                     QFGSNRGSSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISDEQATRELASRST
                     RGSAGMYGDAHAEWGVKPKILGVGGGAGVRGGGRTSIDWNDEDAHQASSGSRASHDAR
                     HDIDARATQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTN
                     MTRSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRR
                     AMAWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQ
                     DSNIRNDVKQQVDNMVTEYRGNIGDTQNSIHGEENIVRGQYSELQNH"
BASE COUNT         1387 a         1321 c         1520 g         1326 t
ORIGIN      
        1 acaacaggat attcaaatga taagtaaaca cagattctct ttaccccgac tggatattac
       61 ggggatgtgg gtattttccc tgggtgtctg gttccatatt gttgcccgtc tggtttacag
      121 aaaaccctgg atggctttct ttctggcaga gcttattgca gccattctcg ttctgtttgg
      181 cgcataccag atactggata catggatagc ccgcgtcagt caggaagagc gtgaggcact
      241 ggaagtcaga cagcagttgt tactgagaga acggaaggat aatgatgatg aacatgtttc
      301 ccgttaaatc gctgtccgtt gtcctgcttt ttctggccag ctgtacacag gcatccacac
      361 gtgcggaaat cgagcgactc tggcatccgg agggaatggc agcacggaca actcagccgg
      421 cagcagacgc acaggtgcga caggagaaag cagcaacccg ctggttccgc ctcagcaacg
      481 gaactcaggt caatcttgct gactggaaag tggtgctgtt catgcaaggg cgatgtccgt
      541 actgtcacca gtttgacccg gtactgaaac aactggcgct gcagtacgga ttttccgtat
      601 ttccctatac cctggatggt cagggtgata cggcctttcc gcaggcgtta cctgcaccac
      661 cggatgtact gaaaacattc ttcccgaata tcccggtggc cacaccgacc accttcctgg
      721 tcaacgtcaa cacgcttgag gcattaccgc ttttacaggg ggcaacggat gccgccggtt
      781 ttatggcgcg gatggatacc gttctgcaga tgtacggagg gaaaaaaggt gcgaaataag
      841 caggtggtgt tactcattgc cggaatatcc ggcgtcgcga cgggaataat cgtcagcctg
      901 aatatcccct ttatccgtca gggacttttt tatcccgcca gccctgcaga aattgtcgtt
      961 tcgctgtgtc ttaccttttc tgtttctgtt gtgtttttgt gggggcaatt gtgggatgga
     1021 tttcagtatc tgaaatatat tacagccgca tgaacggtct gaatgaatca tcagaaatat
     1081 cagaggaaac ttataatgaa agaaaaaaga aatgatgcgg aacaaaaaaa cagaaaaact
     1141 aaacgggatt atgattatga acgcagggtt tctgatatat attttgatct gttttttgtg
     1201 ttcgttgctg ccggaacttt tttatgggtg attatgcaca gtatttttga tgcctgtatt
     1261 gattcgtgga aagcggaccc gaaattactt aatttccact atatgtggaa catcctgatg
     1321 tatgtcattc cgtatacgtt atgggcgttt gcaggtggat ttcttatcgt atacgtcagg
     1381 aaccctttaa atgaattaat aaacgggggg atcaggatat tccggctgaa acgccgtatg
     1441 cgccgggata acaaattaag ggagggtggc aataatgctt cgcattaaac ctcttctggt
     1501 tctttgtgcc gctttactga cggtcacacc tgcagcgtca gcggatgtga acagcgacat
     1561 gaaccagttc ttcaacaagc tgggctttgc caccaatacc acccaggccg gagtctggca
     1621 ggggcaggct gccgggtatg cctatggcgg ttctctgtat gcccgtaatc aggtcagaaa
     1681 tatccagctt atctccatga cactgcccga catcaatgca gggtgtggtg gcatcgatgc
     1741 ctatctcggc tcattcagtt ttattaatgg cgagcagctg cagcgttttg ttaagcagat
     1801 tatgagtaac gcgatggggt acttttttga ccttgccctg cagacaacgg tgcccgaaat
     1861 caaaaccgcg aaagacttcc tgcagaagat ggcgaatgac attaacagta tgaacctcag
     1921 ctcctgtcag gtggcacagg ggattgtcgg aggattattt cctcagactc aggtgtcaca
     1981 gcagaaggtc tgtcaggata ttgcaggtga gagcaatatt ttcgccgact gggccgcgtc
     2041 ccgtcagggc tgcacggttg gggggcagtc atcgtctgtt cgtgacaagg ccagtgacaa
     2101 agataaggaa cgggtgctga agaacataaa tatcatctgg gactcgctgt ccaaaaacag
     2161 gatgcttgat ggtaacaaag agctgaaaga gttcattatg acgctcacag gtacgctgat
     2221 ttttggtgaa gacagcgaga ttacccccct gccggcgcgt accactgacc gcgatatcct
     2281 ccgggcgatt atggaggggg gaacggcaaa aatttaccac tgcaacgatt cggacaagtg
     2341 tctgaaggtg gtggcggatg caaatgtgac catcgcccgg gataaagccc tgaaatcaca
     2401 gattacaaaa ctgctcacca gtattcagaa taaagccgtg agtgacactc cgctggatga
     2461 cagggaaaaa ggcttcattt ccagcaccac cgtccccgtc tttaaatatc ttattgaccc
     2521 gcagatgctc ggtgtgtcca gcagcgtggt ttaccagctg acggactata tcggctacga
     2581 catcatgctc cagtacattc aggaactgct tcagcaggcc cgggccatga ttgccaccgg
     2641 gaactatcca cagaccgtgc tggacagtgt gctggagaat ctgaatcagg cacagcaaca
     2701 gattgcggtt ttccaggggc aggtaaaggt ccagcaggat gcactgctgg ttgttgaccg
     2761 tcagatgagc tacatgcgcc agcagctgtc cgcccgtatg ctcagccgtt accagaacaa
     2821 ctatcacttc ggagggagca cgctgtgaat gaagtctatg tgattgccgg tggtgagtgg
     2881 ttgcgcaata acctgaacgc cattgccgct tttatgggga cccggacgtg ggattccatt
     2941 gaaaaaattg cgctcacatt atctgttctc gcagtggctg tgatgtgggt acaacggcac
     3001 aacgtgatgg atttgctggg ctgggtggcc gtgtttgtgc ttatcagcct gctggttaat
     3061 gtccgcacat cggtgcagat tattgataac agtgacctgg ttaaggttca ccgggtggac
     3121 aatgtgccgg tcggtctggc gatgccactt tcactgacga cccgtatcgg gcatgcgatg
     3181 gtggccggct atgagatggt gttcgctcag ccggacagcg ccacctacag caagacaggc
     3241 atgctgttcg gggcgaacct gattgtgaaa agcaccgatt tcctgtcccg gaatccggaa
     3301 atcatcaatc tgtttcagga ctacgtccag aactgcgtgc tgggtgatat ttacctgaac
     3361 cataaataca cgctggaaga cctgatggcc tcctctgacc cgtacaccat aatcttttct
     3421 cagcccagcc ctttacgtca ggtgccgaat aataattaca gttttctgca aagctcagag
     3481 ccgtttgtgt cctgtaagga cgcatccgtc ggactgaagg ataagctgaa ttttgatacg
     3541 aataccggag gcaagacctg gaattattat gtgcagcaga tatttggcgg caggccggac
     3601 cctgacctgt tattcagaca actggtgagt gacagttaca gttatttcta cggctccagc
     3661 cagtctgcca gccagattat gcgccagaac gtcaccatga atgccctgaa agagggtatc
     3721 accagtaatg cggcccgtaa cggtgatacc gccagcctgg tgaatctggc caccacctca
     3781 tcgatggaga aacagcgtct ggcacatgtc tccattggtc atgtgctgat gcgaaacctg
     3841 ccgatggtac agaccattct ggtgggcatt actattggta tcttcccgct cctggtgctg
     3901 gctgcggtct tcaacaaact gacgctgtcc gtcctgaaag gctatgtgtt tgccctgatg
     3961 tggttccaga cctggccgtt gctgtatgcc atcctgaaca gtgccatgac attctatgcg
     4021 aagcagaacg gggctccggt cgtgctctct gaactctctc agatacagct gaaatactcc
     4081 gacctggcat caaccgcagg ttatctttca gcaatgatcc cccctctgtc atggatgatg
     4141 gtacgaggcc ttggtgccgg attttccagc gtatacagcc actttgcgtc ttcttccatc
     4201 acgccaaccg ccagtgcggc aggcagtgtg gttgacggta attactccta cggcaacatg
     4261 cagacgaata acgtgaacgg cttcagctgg agcaccaaca gcaccacgtc atttggtcag
     4321 atgacgcacc agaccggtag tggtgcaacc gccacgcaga cccgtgacgg cagtatggtg
     4381 atggacgcca gtggggcaat gtcccgtttg ccggttggta ttaatgcaac gcgtcagatt
     4441 gcagcagcgc aacaggaaat ggcccgggag tcgatgaata aggcggaaag tgccctgcat
     4501 ggtttcagca gcagtattgc cagtgcctgg aacacactca gccagtttgg ctctaaccgg
     4561 ggaagcagtg attcagtcac cagtggcgct gacagcacga tgagcgcgca ggactccatg
     4621 atggccagcc gtatgcgcag tgcggtggaa agctatgcga aggcgcataa tatcagcgat
     4681 gagcaggcga caagagaact ggcatccaga agtacgagag gttctgcggg gatgtatggt
     4741 gatgctcatg ctgaatgggg ggttaaacct aaaattcttg gtgttggtgg tggcgcaggt
     4801 gtcagaggtg gtggtaggac aagtattgac tggaatgatg aggatgcgca tcaggccagc
     4861 agtggttcac gggccagcca tgatgcccgt catgatattg atgccagagc tacacaagac
     4921 tttaaagaag caagcgatta ctttaccagt cgcaaggtca gtgaatccgg cagccatact
     4981 gacaataatg ccgattccag ggtggaccag ctttctgcag ccctgaactc agcgaaacag
     5041 agttacgacc agtacacgac gaatatgacc cgcagccatg aatatgctga aatggcctcc
     5101 cgtactgaaa gcatgagtgg gcagatgagt gaagacctgt cacaacagtt tgcacagtat
     5161 gtgatgaaac acgcgccgca ggatgcagaa gctattctga caaacaccag ttcaccggag
     5221 attgcagaac gtcgtcgggc aatggcctgg tcttttgttc aggaacaggt acagcctggt
     5281 gttgataacg cctggcgtga atcccgtggc gatatcggta aaggaatgga gagcgtacct
     5341 tcgggcggtg gcagccagga tattattgca gaccatcagg ggcatcaggg tattattgag
     5401 caaagaacgc aggacagtaa tatccgtaac gatgtgaagc agcaggttga taatatggtc
     5461 acagaatatc ggggcaatat cggggatact cagaacagca tccacggcga ggaaaatatt
     5521 gtaagagggc agtattctga actgcaaaat cacc
//
LOCUS       DAZUCC010000264         5532 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZUCC010000264 DAZUCC010000000
VERSION     DAZUCC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.265"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            67..957
                     /locus_tag="ACHY09_004569"
     CDS             67..957
                     /locus_tag="ACHY09_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepB C-terminal
                     domain-containing protein"
                     /protein_id="HFS3887439.1"
                     /translation="MISQCFDSRFYTFKVESLVYARTLPVNVKFSTKYKQIKASVDEI
                     GLIEPIVVYINDANDKVILDGHLRVEVLKELGITTAHCLISPVEDTYSYNKRVSRLTI
                     VQAQNMLLKAVESGVSVEKLCAVLGVTPDTLIGKLKISDGIAPEVMALLAEKNVPQTI
                     FSVLKKLKFYKQIEMVNTMISINNFTRKFAMSMLHAVAPEHLVAPKKDASRETDIRKN
                     LERLEKEMASVQVETQHLQDEYAENNLRLVIIKNHIERLLANGQVLNWLYDNHQEYLS
                     VLKQISGLDDLNKLGQTIEE"
     gene            1196..1615
                     /locus_tag="ACHY09_004570"
     CDS             1196..1615
                     /locus_tag="ACHY09_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887440.1"
                     /translation="MANQQAAKWAGVAAIAACLTFVVTTIGLLLAWRSLHQWKPQYKE
                     NSRLLLIEALIAFQKCLITIPKNLDNDPTYQSRKEFLKASTEVELRGQIYLKQHSNEK
                     LKDELANLRSKCAEFVGGKVTKPELSFISAIILLIEV"
     gene            complement(1718..2509)
                     /locus_tag="ACHY09_004571"
     CDS             complement(1718..2509)
                     /locus_tag="ACHY09_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HFS3887441.1"
                     /translation="MKKVMLNELTGREIRELLQSGKELTAIVNFGSCENHADHLPLGP
                     DMFVPEIVACRVAQRVDNTLVLPCTPFGTSVHYNAYPLAVTLRYETNIALAEDIFISL
                     LHHGIRRILVINGHDGNIPAIEIAARNVKARHTQAAIVVVPAWWDLVGHHLGKGFFDS
                     WDNTGLGHGGEGETSVMMALIPELVELKYAQKQIPVEVFADYGVNRIWDIGEISATGA
                     TGDPRCATAEKGQRILACLVDVLCALLRKLDAANWQYALNKDANM"
     gene            complement(2487..3845)
                     /locus_tag="ACHY09_004572"
     CDS             complement(2487..3845)
                     /locus_tag="ACHY09_004572"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3887442.1"
                     /translation="MELNRSLKARHLTMISVGGVIGAGFFLGAGAAIARTGPSVVLAY
                     LIGGVITLFIMLLLAEMAVARPVSGSFESYARTAFGPWCGFITGWTYWLAFLIGPASE
                     AIAAGTFLNLWFPGVPVWLFCLVIASSLTAINMVGVHFFGEVEFWLSLVKVVALLAFI
                     VAGCYSLGLDNASPASISHPTDSGSFFAGGLPGFLGAMLMVIFSFGGTEAIGTAAGES
                     ENPQRDIPRTLRGTVVRILLLYVGSLTVLLLVLPWQQAGVSSSPYVEASGILGGHMAR
                     HIMNFVVLTAALSCIDTGVYATSRMLHAMASDGYFPAWFARLHPAHKTPDNAILASSL
                     VLFTGVIIAILSPDAYVILAGISGFGFLFTWLMIALSQPTLHRIALHDGTLKYKAPAA
                     RYIQPLTVGLIALIMAGQFFTDGGGITLVTVLIWLALASAYYIFVVRKNRRLSDEKSH
                     AQ"
     gene            complement(3889..4263)
                     /locus_tag="ACHY09_004573"
     CDS             complement(3889..4263)
                     /locus_tag="ACHY09_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS3887443.1"
                     /translation="MMTYCARIVAIGLFVADGLADRTLITFDSNGPKDCLDYSLSLEP
                     SFREDDLLILPGDRLLLASHDYLVTAVGKEAQQSLFELGHLTMVFNGSLNACHSGAVH
                     LSGPIPKLHDLYGELLIEEGRP"
     gene            complement(4482..4757)
                     /locus_tag="ACHY09_004574"
     CDS             complement(4482..4757)
                     /locus_tag="ACHY09_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3887444.1"
                     /translation="MTKKDWHPADIIAALHKRGTSLAKLSREAGLASSTLANALNRPW
                     PKGEWLIAIALGKHPSEIWPERYLNTGNKREPRKPLHLMTTTENSGT"
     gene            4865..5128
                     /locus_tag="ACHY09_004575"
     CDS             4865..5128
                     /locus_tag="ACHY09_004575"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3887445.1"
                     /translation="MEKTGSIKKVLGKRIRALRREMDLSQEALADKCGIDRTYMSGIE
                     RGVRNPTLVVICAISDGLGMDLSHLFMFDDGAEKDAESTKCDS"
     gene            5168..5392
                     /locus_tag="ACHY09_004576"
     CDS             5168..5392
                     /locus_tag="ACHY09_004576"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3887446.1"
                     /translation="MKNTSSIQAQFGAHLKKLRLQSGLSQEAFADKCGLDRTYVSGIE
                     RGVRNPTLEVISVLGKGLDMDIKELFNFER"
BASE COUNT         1428 a         1328 c         1486 g         1290 t
ORIGIN      
        1 acagtaataa tatccacgat attccttctt tactggatgg cagtgatgta tcgggggaga
       61 aataagatga tttcacaatg ttttgactcc cgattttata cctttaaggt ggaatcactg
      121 gtgtatgcca gaacgctgcc ggttaatgtt aaattcagta cgaagtacaa acaaattaaa
      181 gcctctgtcg atgaaattgg gttaattgaa cccattgtgg tttatatcaa tgacgccaat
      241 gataaagtca ttctggatgg acatcttcgc gttgaagttt tgaaagaact cggaataact
      301 actgctcatt gtctgatatc ccctgtggag gatacatatt catataacaa acgtgttagt
      361 cgccttacca ttgtgcaggc ccaaaacatg ctgcttaaag cggttgaatc tggcgtttca
      421 gttgaaaaac tatgcgccgt actgggtgtc acgccagata cgctgattgg taaactgaaa
      481 atttctgatg gcattgcccc tgaagtcatg gcgttactgg cagaaaaaaa tgtaccgcag
      541 accattttct ctgtgttgaa aaaactaaag ttctataagc agatagaaat ggttaacacc
      601 atgatcagta ttaataattt cacccgcaaa tttgcgatgt cgatgctgca tgccgttgcc
      661 cctgaacatc ttgtagcgcc taaaaaagat gccagcagag aaacagatat cagaaaaaat
      721 ctcgaaagac tggaaaaaga aatggcttcc gttcaggtgg aaacgcagca tcttcaggat
      781 gaatatgctg agaataacct tcggctggtt atcattaaaa atcacattga acgtctgctg
      841 gcgaacggtc aggtacttaa ctggctttat gataaccatc aggagtacct ttctgtgctc
      901 aagcaaatct cggggctgga tgatttgaat aagcttggcc agacaattga ggaatgacac
      961 atttttaaag caaggactgc tgttttacag attgccatta tgatgggatg cactgagaga
     1021 ggagggcgtg ctggtggttg acgtgattca actggtgcgg aaaatcttgc tcaaaggacg
     1081 atggcttaaa atcaagttgc tgtttcaaaa atgaaaaagt agatttacat ccacttttct
     1141 tactgaggta aaaatagtgc atatagatgc ggatttcata agcttgagca cattggtggc
     1201 taatcaacaa gctgcaaaat gggcgggggt tgctgctatt gctgcctgtt tgacgtttgt
     1261 tgtgacaacg ataggtttac tacttgcttg gcggtcgttg catcagtgga agcctcaata
     1321 caaagaaaac tctagacttc tcctaatcga agctttaatc gcatttcaga aatgtttaat
     1381 aactatacca aaaaatctcg ataacgatcc cacctatcaa agccgaaagg aatttttgaa
     1441 ggcaagtact gaagttgagc taagaggaca aatatatctt aagcaacaca gcaacgaaaa
     1501 attaaaagat gaattagcaa atctcaggag taaatgtgca gaatttgttg gtggtaaggt
     1561 gactaaacct gaactgtcat tcatctcagc aataatatta ctcattgaag tataatagat
     1621 tctgggctaa caccaatcgg ttacccggtt gggatatcgc ttccgggtaa cctgtggaaa
     1681 ggcaaaagcc actccacagg ttgcccgagc atatcgatta catgtttgcg tctttattta
     1741 acgcatattg ccagttagct gcatccagct ttctcagcaa cgcgcaaagc acatccacca
     1801 gacaggcgag gatccgctgg cctttttccg ccgtggcgca gcgcggatcg ccggttgctc
     1861 cggtggcgct gatttccccg atatcccaga tccggttcac gccataatca gcgaacacct
     1921 ccaccgggat ctgtttctgc gcatatttca gttccaccag ctccggaatc agcgccatca
     1981 tgacggacgt ttcgccctcg ccgccgtgtc ctagtccggt gttatcccat gagtcgaaaa
     2041 atcccttccc gagatgatgt cccaccagat cccaccacgc cggaaccacg acaatcgctg
     2101 cctgcgtatg tcgggccttc acattgcggg cggcgatttc aatcgccgga atattaccgt
     2161 catggccgtt aatcaccagg atccggcgaa taccgtggtg cagcaggctg atgaagatat
     2221 cttccgccag cgcgatattg gtctcgtagc gtagggtgac cgccagtgga taggcattgt
     2281 agtgaacgga tgtgccgaac ggcgtgcagg gcagcacaag ggtattatcc acccgctgcg
     2341 ccacccggca ggcgacgatt tcgggtacaa acatatcggg gccgagtggc aggtgatcgg
     2401 cgtggttctc acaactgccg aagttgacga tagcagtgag ttccttacca gactgcaaca
     2461 gctcgcggat ttcccggcca gtcagttcat tgagcatgac ttttttcatc ggacagcctc
     2521 ctgttttttc ggacgacaaa gatgtagtac gcgctggcga gggccagcca gatcaggacg
     2581 gtaaccaggg taatgccgcc gccgtcggtg aaaaattgcc cggccatgat cagcgcaatc
     2641 agccccacgg tgagtggctg gatgtagcgg gcggccggag ccttgtattt cagcgttccg
     2701 tcgtgcaggg ctattcggtg cagggtgggc tggcttaagg caatcatcag ccaggtgaac
     2761 agaaagccga atccggaaat cccggcgagg atgacatacg catctggcga gagaatggcg
     2821 atgatgacgc cggtaaacag caccaggctg ctggcaagaa tggcgttatc cggtgtttta
     2881 tgtgccgggt gcaggcgggc aaaccaggcc gggaaatagc cgtcagacgc catggcgtgc
     2941 agcatccgcg aggtggcgta aaccccggta tcgatgcagg agagcgcggc ggtcagcacc
     3001 acaaagttca tgatatgccg cgccatatgg ccaccgagaa tgccggaagc ttcgacatac
     3061 gggctgctgc tgacacctgc ctgctgccag gggagaacga gcagcagaac ggtcaatgag
     3121 ccgacataca gcaacaggat ccgcaccacg gtgccacgca gcgtacgagg aatatcacgc
     3181 tgtgggttct cgctttcacc cgcggcggtg ccgatggcct ctgtcccgcc gaaagagaaa
     3241 atcaccatta acatcgcgcc gagaaatccg ggcagcccgc cagcaaaaaa gctgccgctg
     3301 tcggtcggat gactgatgct ggcgggtgag gcgttatcca gtcccaggct gtagcagccc
     3361 gcgacgataa aggccagcag cgccacgact ttcaccaggc tcagccagaa ctccacttca
     3421 ccgaaaaaat gaacgccgac catgttgatg gcagtcagcg acgaagcgat caccaggcaa
     3481 aacagccaga cggggactcc gggaaaccac aggttcagga aggtgcctgc agcgatggcc
     3541 tccgacgccg ggccaatcag gaaggccagc cagtaggtcc acccggtgat aaagccacac
     3601 catgggccaa aggcggtgcg ggcataggat tcaaaggaac cggagacggg cctggcgacc
     3661 gccatttcgg caagcaacag cataatgaac agggtgatga caccgccgat aaggtaggcc
     3721 agcaccaccg acggcccggt acgggcaatg gccgcgcctg cgccgaggaa aaatccggca
     3781 ccaatcacgc ctcccacgga aatcatcgtc aggtgacggg ctttcagact tctgttgagt
     3841 tccataatgt cttctctttt gttgttgtgg atggattcaa ggcagcgctt atggccgtcc
     3901 ctcctcaatc agcagttcac cgtacaggtc gtgaagttta gggattgggc cggagagatg
     3961 aacggctccc gaatggcagg cattcaggct gccgttaaac accatggtca ggtggccaag
     4021 ttcaaacaaa gactgctgtg cctctttacc cacggcggtc accaggtagt catggctcgc
     4081 cagcagcagc cgatcgccgg gcaggatcag caggtcgtct tcgcggaatg agggttccag
     4141 cgacagggaa tagtccaggc agtctttcgg tccgttgctg tcaaaggtga taagcgtcct
     4201 gtctgccagt ccgtcggcga caaacagccc gatagcaacg atgcgtgcgc agtaggtcat
     4261 catggctcgt tctcccgttt gtcgggcatg agagtacggc ctggtgtagg tgagcaggca
     4321 tgtagcctga ataggtgaat aaagccttca gtccgggagg ccgggtgaag gtggcctccg
     4381 gttgtaagag cgatggagca agggtgtggt tcggtgtgca taaacaactc tcctgtcagg
     4441 tgtcgttgat taatgcgtcc tgccactcac agcgcgggtc gttatgtccc ggagttttct
     4501 gttgttgtca tgaggtgaag cggtttacgg ggttcacgtt tatttcccgt attgaggtaa
     4561 cgttccggcc agatttctga cggatgcttt cccagcgcaa tggcaatcag ccactcacct
     4621 ttcggccacg gtcggttcag ggcattcgcc agcgttgacg aggccagtcc cgcttcccgc
     4681 gagagtttcg ccagactggt gccgcgcttg tgtaaggcgg caatgatatc ggcggggtgc
     4741 cagtcttttt tagtcattcc gtttacctct attgttgact atgagtcacg aatgtatgct
     4801 agcacgcttt ttatgtgact catagtccgt ggactgataa tcaacgaaga ttgatgctgg
     4861 ctagatggaa aagacgggat caatcaaaaa ggtactgggt aaaagaatca gggctctacg
     4921 ccgtgagatg gacctatccc aggaagcttt agccgacaaa tgcggtatcg atcgcaccta
     4981 tatgagtgga attgagcgag gagtaaggaa ccccacgctt gttgtcattt gtgcaatctc
     5041 ggacggtctg gggatggatc ttagccattt atttatgttt gacgatggcg cagaaaagga
     5101 cgccgaatca acgaagtgtg actcatagtc cgtggtctga tagacaacgt aagttcattt
     5161 tatctggatg aaaaatacat ccagtattca ggcgcagttt ggcgcacatc tcaaaaaact
     5221 cagactccag tcgggcctgt ctcaggaagc attcgcggat aagtgtggtc tcgatcgtac
     5281 ttatgtgagc ggtattgagc ggggagtaag gaatccaacc cttgaggtga taagtgtttt
     5341 aggaaaaggg ttggacatgg acatcaaaga attattcaac tttgaaagat aggtttatct
     5401 attagtagca actctatccg cagcagtacg ggtaacttga tttccaacat cccagccgtt
     5461 ttcatctggg agtgacatcc catccattgg cattaggata aaaccaccaa tagttggtcc
     5521 aaatgactca gt
//
LOCUS       DAZUCC010000265         5528 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZUCC010000265 DAZUCC010000000
VERSION     DAZUCC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.266"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..810
                     /locus_tag="ACHY09_004577"
     CDS             <1..810
                     /locus_tag="ACHY09_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFS3887447.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKLLNEAVLFSAPEGMALT
                     SGEDMQMSAARNVAVNAGGDISTGTMGNLTALAGEKLGLFARTGQLSLKSGEGAVDVQ
                     AQNGSMRLFAEKKLTISSESDISFTGKKRITLIGGGSYLRLEAGKVEYGTMATCMRRV
                     KRTMTEAAQSMPMTFPRMSSSHIYSAIYRLLDKHGNILANTPCRLTTPSGQTVMGYSD
                     NEGRSVPVYTRQQEDVELHFFVKKPQPEETMWFVGETDTQQLETELRESQS"
     gene            810..2093
                     /locus_tag="ACHY09_004578"
     CDS             810..2093
                     /locus_tag="ACHY09_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFS3887448.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGRNRTRMTILEIHDCRPEE
                     HQWVDQVVNVTMKAWQISGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKVVSDL
                     SEGAVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVKSW
                     TNTQIKAAWTEIQHVTDMTLDMLKQVDWVQVLTNVAKTVGVILVAVGVGGLVVTLGIP
                     EAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVS"
     gene            2106..3320
                     /locus_tag="ACHY09_004579"
     CDS             2106..3320
                     /locus_tag="ACHY09_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HFS3887449.1"
                     /translation="MELHDFELNYIEKWLPEASVKYKDGSPAVNLGLIITVFFKDGHT
                     PEVRRRMVECVDRFYGEFKPWLKKTITKNWVGITDNNYAKKRQEILDSTPEEIFSWYL
                     SSAEKDFLAPDYSILIMGKRIFHNDNNRSVIKLTLPLSLLKETDGKQRYEAWLMWLCD
                     TFSVESGYAGLSFALPYARERMFPCEFALAQRFSGVMVDSLGTLEGGEAVEGLKGACW
                     YTILGTPWLEKLGGAERVKHRLAKTPEISLLPYNNGVVLKAGELPPPLGEMKTEGLSP
                     LLVKVNQVIRSIRYDGRNGLHFYSEYEGLQFEEASSMAWFARFDEASALLDKEEMGKS
                     CAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQKMPPFEDKHGKQHRACWKLLK
                     RDDNGSVYTLPE"
     gene            3334..3687
                     /locus_tag="ACHY09_004580"
     CDS             3334..3687
                     /locus_tag="ACHY09_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFS3887450.1"
                     /translation="MTVQAGERLGLFARTGQLSLKSGEGAVDVQAQNGSMRLFAEKKL
                     TLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTMATYLRRTKRTMVAAPATMPLP
                     IPLLLDLYPPLNGQI"
     gene            4016..4471
                     /gene="tssE"
                     /locus_tag="ACHY09_004581"
     CDS             4016..4471
                     /gene="tssE"
                     /locus_tag="ACHY09_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFS3887451.1"
                     /translation="MSHHSPSLYETLYGNFTGGLDLHQVCEENQVIVSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTTILQGMPGTAHQLMRVLSDVLLKYEPRIKRVDVTMQEQTQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRHLKQQQFVDNSSYYHV"
     gene            4539..4844
                     /locus_tag="ACHY09_004582"
     CDS             4539..4844
                     /locus_tag="ACHY09_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887452.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRVFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            4899..>5528
                     /locus_tag="ACHY09_004583"
     CDS             4899..>5528
                     /locus_tag="ACHY09_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFS3887453.1"
                     /translation="MTSEVKLKTGGDPRSFPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTASWYTIARMHTTGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFNRLQKTLRAMPPDDRDNLPLLYQTETFLKALSDTLARHELKQSSKVALPE
                     TMVKGYITRLENQPMQGEVSSPVTLPARALRSDAPDVQGHQPLPHSRLVY"
BASE COUNT         1398 a         1307 c         1538 g         1285 t
ORIGIN      
        1 ctggaaaatg ccgcagaacg ggcacaggcg ctgaaggctg atattgacag tcagatacag
       61 atgtttgagc agcggctgaa actgctcaat gaagcagtgc ttttctcggc accggaagga
      121 atggcgctga ccagcggtga agatatgcag atgagtgcag cacgaaatgt ggctgtcaac
      181 gccggggggg atatcagtac cggaacaatg ggcaacctga ccgcgctggc cggagagaag
      241 ctggggcttt ttgcccggac cggtcaactt agcctgaaat ccggggaagg ggcagtggat
      301 gttcaggcgc aaaacggcag catgcggttg tttgctgaga agaagctgac catttcgtca
      361 gaaagtgata tctcgtttac cggcaagaaa cgcatcacgc tgattggcgg ggggagttac
      421 ctgcggcttg aagccgggaa ggtcgagtat ggtacgatgg caacctgtat gcgtcgggtg
      481 aagcggacga tgacggaggc tgcgcagagt atgccgatga cattcccccg tatgagttct
      541 tcgcatatct actcggctat ttatcggttg ctggacaaac acggcaatat tctggccaat
      601 accccctgtc gcctcaccac tccatctggt cagacagtaa tggggtatag cgataacgaa
      661 ggccgctccg tgccggttta cacccgccag caggaggatg tggaactgca tttttttgtt
      721 aaaaaaccac aacccgaaga aacgatgtgg tttgtggggg aaaccgatac gcagcaactg
      781 gaaaccgaac tcagggagag ccagtcataa tggaaatgcc cgatttaccc gagacaccga
      841 catctgatat tcccactggg gcgaacaacc agccgtggga ctgcaccacg acgggtgtcg
      901 aaatggtcga aaagcagtcg cgtctgccaa ccacggaaaa ccgtcagtac ctgcatataa
      961 aggtggaata tgccatgcgt tttccgcaac tgtcggcaca aatccgccgg aaaggtaaaa
     1021 cttaccagat gagccttaag cagaccgtta tgagtggtct tattcgcgtg gatgaaatcg
     1081 cccgcaatta ctgctggtat tataaggcgg aggtggcgtt tgatatgcgc accacgccgc
     1141 cacgcccttt tctgagcagt acgctgctgg aaaaagacgg tgctggccgt cgccacacga
     1201 ccaatccttt ccctgtgccc ggcaggggca tttaccgccg cccggacgtg attattgtta
     1261 aaaataagga agaccgctgg cccggactgg cggggccgga taccgaaggc aatatgcaca
     1321 gcgataacct ggcccgcctg gtggaggtga agtttccggg agataggctg tatgaagacc
     1381 aatatgaaga gtatatgcag attgtgggca ggaaccgaac ccgcatgacc attctggaga
     1441 ttcacgactg tcgcccggaa gagcaccagt gggttgacca ggtggtcaac gtgacgatga
     1501 aagcctggca gatcagcggc gcgaaatact ggccgctggc tctttacccg ccagtcttta
     1561 tgccccgtcc gccatcaacc cccgttgctg ccagaataga gccctggact tacgctggta
     1621 aagtagtatc tgacctcagt gagggggcgg tgcgtggtgt ggctgaaggc tggaatgccc
     1681 tgtcaaaaga gacgcaactg gtcctgaatt cagcagccag ctggcttaac gacagcggtg
     1741 agtgggttta ccggcaggga agtgaggtct gggtctgggc cagccaaacc ggtaagtcgg
     1801 tgaaatcctg gaccaacacc cagattaagg ccgcatggac agaaatacag cacgtcaccg
     1861 atatgacgct tgatatgctg aaacaggtgg actgggtaca ggttttaacc aacgtcgcga
     1921 aaacggtcgg cgttattctg gttgctgtgg gtgtcggcgg actggtcgta acgctgggta
     1981 tcccggaagc cattattggc gcgtttttac tgattgttcg tctggcaata agcgcctggg
     2041 gaacactggc gacgattctc ggtacgggta cggcggtact ggcagttagt taaacaggag
     2101 caggtatgga attgcatgat tttgagctga actacatcga gaaatggctg ccggaagcct
     2161 ccgtgaaata caaagacggc agtccggcag tcaatctcgg tctgattatc accgtctttt
     2221 ttaaggacgg acatacgcca gaagtccgcc gcaggatggt ggagtgcgtg gatcgcttct
     2281 atggggagtt taaaccctgg ctgaaaaaaa cgatcaccaa aaactgggtt ggcatcactg
     2341 ataataacta cgccaaaaag cggcaggaga tcctcgattc aacgcctgaa gagatttttt
     2401 cctggtatct gagtagcgca gagaaggatt ttttagcccc cgattacagt attttaatta
     2461 tggggaaaag aatttttcat aatgacaata atcgttcagt cattaagtta acactcccgt
     2521 tgtcactgct gaaagaaact gatggaaagc agcgttatga agcctggctg atgtggttgt
     2581 gtgacacctt ttcggtggaa agtggttatg cagggttgtc gtttgcactt ccttatgccc
     2641 gtgagcgaat gttcccctgt gaattcgccc tggcgcagcg tttttccggt gtgatggtgg
     2701 attctcttgg gacgcttgaa ggcggtgaag ccgttgaggg gctgaaaggt gcctgctggt
     2761 acaccattct cggtacgccg tggctggaaa aactgggggg ggcagagaga gtaaaacatc
     2821 gtttagctaa aacgcctgaa attagcctgc taccctataa caatggtgtc gtacttaaag
     2881 ccggtgaact ccccccgcca ctgggagaga tgaaaacaga aggtttatcg ccgctgctgg
     2941 tgaaggtgaa ccaggtcatt cgttccattc gctatgatgg acgcaatggt ctgcattttt
     3001 actcagaata tgaaggactt cagtttgaag aagcgtcttc aatggcatgg tttgcccgtt
     3061 ttgacgaagc cagtgccttg ctcgataaag aagaaatggg gaaatcttgc gcccccgaac
     3121 gcattaaatg ctggaccgat gaaatagcgc cccatgccgg acaatgggcc gcgatggtta
     3181 atggcaccac cgagtacatc cagacccgcg aagggcagaa aatgcccccg tttgaggata
     3241 agcacggtaa gcagcacagg gcttgctgga aactactgaa acgagatgac aatggtagcg
     3301 tttatacact acctgagtaa ggtcgtgtgc tgaatgaccg tgcaggccgg agagaggctg
     3361 gggctttttg cccgcaccgg tcaacttagc ctgaaatccg gggaaggggc agtggatgtt
     3421 caggcgcaaa acggcagcat gcggttgttt gctgagaaga agctgacgct gagctcagcg
     3481 agtgacatct cctttgccgg gaaaaaacgc atcacgctga ttggcggggg aagttaccta
     3541 cggctggagg cggggaaaat agagtacggt acgatggcga cgtatctgcg gcggacgaaa
     3601 cggacgatgg tagcagcccc ggcaacaatg ccgctcccca taccgcttct cctggacctg
     3661 tatccaccac ttaatggcca aatttgaatt gaatgtctcc tcaatgccat caaagtcaac
     3721 gatgctatcg ttcagggagc gtagtcaatg gatgtcacaa aaatgatgca ggtgctacag
     3781 gatttgtttt tatgctgcta aatgtacagc gacttcgtga tatggggttg ttggtgtttt
     3841 ttataataat gctttttacg gaatcaaaaa aacagacaac atattaatta tttgagaaag
     3901 cagcgtcaaa aaggaaaagt taaaatgtcg aatcgatttt ttactccggt aatcttcttt
     3961 ccagtgtgtt gattgaacta tttccaagat taaatgatta tgagaagaag tcgtgatgtc
     4021 tcatcatagc ccctcccttt acgaaacctt atacggaaat tttaccggcg gcctcgacct
     4081 ccatcaggtc tgtgaagaaa atcaggtcat cgtgtcggtg ctcgataaca tgcagcgtat
     4141 tctgaatacc cgggccggaa gccttaaaca tcttccggac tatgggctgc cggatatgac
     4201 aaccattctg cagggaatgc cgggtacggc gcatcaactg atgcgcgtgc tttcagatgt
     4261 tctgctgaag tatgagccac gtattaagcg tgtcgacgtg acgatgcagg aacagactca
     4321 gcctggtgag ttgcattatg tcattgatgc agaacttaaa gatgccgggc tggttcgtta
     4381 cggaacaaca tttatgccgg agggcagagt gttgctgcgc catcttaagc aacagcagtt
     4441 tgtggacaat tcatcttatt atcatgtctg atttttttat ccgggctgcc cctgtgtggg
     4501 tggtccggat gaaaaatata ttttttgtga ttttgattat gaacccgtta tttaacgata
     4561 ttcagatgcg tcttttttat ctgaaccact ctccgtactc ctggcactgg aatgtcagat
     4621 tcagacctca ggaagccgta tatattggca gcgatacctg tcatatcacc attacctgta
     4681 atcagtccgg ttttcattta acccgggacg gacaacgggt attcactgag cgttatatca
     4741 gaaaccttaa tgagctgtta ccagttctga aacggcggtg ggatgtgaca ccggcaatca
     4801 tccgtgcagt ggaatacctt tcccgggtcc cggtatcaca ctgacattct ttccgtaact
     4861 gttaatcatg atttaataca gcaacactga atctgccgat gacttctgaa gtgaaactga
     4921 aaacaggtgg tgacccccgg tcattcccgg attatgctgc cctgcgcgat gaaatcagca
     4981 aactgaccca cccggcacgc ccggatgtgg actggcgtta tgtggaaacg ctctgcctgc
     5041 gcctgtatga acacaatggc gtggaattac agacagcatc ctggtacacc attgcccgga
     5101 tgcatacaac agggttaagt ggcctgaatg aagggctggc tctgattgtg gcgctgaccc
     5161 ggcatcactg gtcagtgatg tggccgctga atacccacgc ccggctggaa attattaccg
     5221 gtctgttcaa ccgtctgcaa aaaacgctcc gggccatgcc accggacgac cgtgacaacc
     5281 tgcccctgct gtatcagacc gaaacattcc tgaaggcact gagtgataca ctggcccggc
     5341 acgaacttaa acagagcagt aaagtggctt taccggaaac catggtgaag ggatatatca
     5401 cccgtctgga aaatcagccg atgcagggcg aagtgtcgtc tccggtaacc cttcctgctc
     5461 gggcactccg gtctgatgct ccggatgtac agggacatca gcccctgccg cactcccgtc
     5521 tggtgtat
//
LOCUS       DAZUCC010000266         5489 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZUCC010000266 DAZUCC010000000
VERSION     DAZUCC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.268"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..153)
                     /locus_tag="ACHY09_004584"
     CDS             complement(<1..153)
                     /locus_tag="ACHY09_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFS3887454.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASV"
     gene            complement(150..830)
                     /locus_tag="ACHY09_004585"
     CDS             complement(150..830)
                     /locus_tag="ACHY09_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFS3887455.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTLSPRYIW
                     TEPQEEQK"
     gene            complement(809..1528)
                     /locus_tag="ACHY09_004586"
     CDS             complement(809..1528)
                     /locus_tag="ACHY09_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFS3887456.1"
                     /translation="MKRKILYLLALCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPGILPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            complement(1532..2281)
                     /locus_tag="ACHY09_004587"
     CDS             complement(1532..2281)
                     /locus_tag="ACHY09_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887457.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESAREKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
     gene            complement(2278..2877)
                     /locus_tag="ACHY09_004588"
     CDS             complement(2278..2877)
                     /locus_tag="ACHY09_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFS3887458.1"
                     /translation="MKKRFILSGLTVCLLMAGCQGTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            complement(3063..3983)
                     /locus_tag="ACHY09_004589"
     CDS             complement(3063..3983)
                     /locus_tag="ACHY09_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFS3887459.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRQEEREKALQENYAS
                     ARRMQQMGISSEKIKEILQFSDDDLRNALR"
     gene            complement(4032..4592)
                     /locus_tag="ACHY09_004590"
     CDS             complement(4032..4592)
                     /locus_tag="ACHY09_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS3887460.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKEHTEWHRVVIYGKLAEIAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYST
                     EVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGM
                     PPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            complement(4608..4811)
                     /locus_tag="ACHY09_004591"
     CDS             complement(4608..4811)
                     /locus_tag="ACHY09_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887461.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(4902..5369)
                     /locus_tag="ACHY09_004592"
     CDS             complement(4902..5369)
                     /locus_tag="ACHY09_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFS3887462.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT         1253 a         1633 c         1321 g         1282 t
ORIGIN      
        1 aactgaagcg ggaaccggtg gctgccggtt tatcgcctca aaaaaggggc tggcatccct
       61 gccgcccagg tctgcaatca ctttcagttc tgcgccagct gttgtacaga acagcagcag
      121 actcagtaca taaaactgtc tttctcgttt catttctgct cctcctgtgg ttcagtccag
      181 atataacggg gggatagtgt cagcatggcg gtcgctgcca catgcacatc agacggattc
      241 aggaaattat cccggtggag ccgggcaaga tgccgtctga ccacagcggt ataacgtgcg
      301 gccggcgcac cacctgccgg gtggtgataa cacccggcca cctgcagcca gctgaccgga
      361 tggcgacgcc ggcattcctg cagaatgacc gctgccgtat tcagactggt gtaaggatca
      421 aatgccgccc atgtggacgg gaaacgatgt ccattccagc ccagattgac ctgtgctatc
      481 ccgacatcaa tacgttttgc cggataatgt ttcagaaaca cctgcaatgc ctgccaggcc
      541 tccagacgtg tggcataacg gtaccctttt cctgccacat ttattgtcca gggccagggt
      601 cgggccacgt ccggctgacg aaaatcacga cgccgttgtt tcagtactcc ggacatcgct
      661 tccggagaca tggacgtttc cgtcagggaa acggcataca aaagctctgc gggaatacca
      721 tgggcacggg ccacccgcac gtacccgtcc ggtacgttct gaacggcagc acgacagaca
      781 ccactgcagg ccaccatcag cagaaccgtc agaatgccgc cacgcgccat ccgtcagccc
      841 cctgctgcag taccaccggc atttttcctt caccaaaacg cagccagcgt ccggcatcat
      901 gattcagggt gatatgacgg ctgcgaactt tctccacagg tatgctgtgc gcatgcgccc
      961 actgacgcag aatctcatcc tttccctgac tgtcgacaag gtagatatca acctgccgtc
     1021 ccgtggccag tacggaggca agacgggcat cacaggtgcc acattccttc gccttcacaa
     1081 acagcgccag ccgtcccccc gtatctccgg taagtgctcc tgtttttccc atactgacag
     1141 gcaagatacc gggatacagg cgcttccagg ccgcattcac ctcctgctga aacgccagct
     1201 ccttctccgt tcgttcatat tcagccctga cccacttttc ggcccagaca cgtcgctctg
     1261 ccggcgttct ggcctctata ccgagaaccg tcagcggatc aagtccggga gactgtatcc
     1321 cccgggggcc ggacatcagt gactgataac gccggtaatc ctcctcactc acaccccact
     1381 gcccggcctg ctgctgaagt ccggatgtgg tactctgcgt caccaccgta ccggtcgtcc
     1441 gttgcgcctc tgacgacata ttcgtctgcc cggcggactg agccagaacg gggcacagtg
     1501 ccagcagata cagtattttt cgcttcatca tctactccac cctgagcacc gtctgacgac
     1561 cattcacccg gaacgttgct tcaccatggc cggcatacac aagcgtccac ccggccacac
     1621 tctctccttc acccagcaga gccacctgag agaggctggt atacccccgg ggagccacgg
     1681 ctgcacgggt actgccaccg cgcagctctg tcccggtcag cacaaaagga gcctgccgaa
     1741 ctgtatgaac tgcgcgggat ttacgggagg acgtcaccgg agcaggcttt ttctcctccg
     1801 tttttttcat ccccgctttc ctgctgtccg gctgtgacgg tgactccggt actttctgca
     1861 tcttctccac cagaccgctg agtgtatcca gtctcaggga aagcttttcc cgggcagact
     1921 caatctgctc cagcatctcc ggcagattct ccagttgttc cttccaggat gctgtcagca
     1981 cgggctgctg ctccaggact gccaggcgct tttccatatc cttcagttgc ttctccgtct
     2041 ctgtcagttt cattctcagg ggcgcgagtt ctgacgcaga aagcatttct cttctctggc
     2101 cttccagggt cgtgatccgg ctgtcaagac cggtcataag catcagtacg ccatatacca
     2161 ctgcccccat gaccagcccc acagtgacag acagtatcaa cacgccgggg aaacgacttt
     2221 ttcttcgctc cggcgacata ccagacgcac cctccggtat ctgcggctgt ttctcactca
     2281 ttttttcaga aaccctcctg ttgccttcac acccggttca cgacccacaa aaggaacggt
     2341 gaccggaaca ggagccagct ggctgaccgg taactggtaa ccgtcacgca gactgtggca
     2401 gaccaccctc tgcacatcat ccacttccag ttgccatgcc ggccccgcca ttaactgcag
     2461 ggcagtacgc agtctcagtg gtcctggctg atactgcacc gccggcagag gctgacggta
     2521 cagcacgccg ttggccggtc cggtggcgca gagcgtgtaa ccggactgtt tcagggcata
     2581 acgcatggca tcggccaccg tgggtttcag ggaggaggga atgcgaatgt cgatcacctg
     2641 agaaagtgga tcacgctgta cagcctgcgg ctctgtgctg accagcaggt aacggtcata
     2701 gcgcaccacc tccggtgcct gagaatacac atccgggctg gcaggctgta cattacggtt
     2761 tactgtgacc gacggtaccg gcatggccgc atcccgttgc tgtagtttct gagtgccctg
     2821 acatcccgcc atcaggaggc agactgtcag tccggacagg ataaagcgtt ttttcatcgg
     2881 gtatttacct ctgcagtgat aaatcagagg tcaatgtgca gaagaagacg gggctggctc
     2941 aatgaacaac tcattacccg ctggcgaaaa aaaacgcctg cagacatgca ggcgctttgt
     3001 tgacaggaag gaaagcaaca taacaaatat gtcacaatcc tgcggttaac ataaattacc
     3061 gattaccgga gagcattacg caaatcatca tccgagaact gcagtatttc tttgattttc
     3121 tctgacgata tacccatctg ctgcatgcga cgggccgatg cgtaattctc ctgaagagcc
     3181 ttttctctct cctcctggcg gcccttctcg ataccagcca gttccagtcg ttcagcgata
     3241 gtcattagat catcctcatg tcttggcaga cgttctgcca tcgcccgtat aaattcaggt
     3301 gaagctgaat cacattcttt caccatatag ttaaacaatg tcaccacctg agtgtgtgtt
     3361 tcctgtatct gaatcaactg tgacaaaaag tcaagatgaa ccatcagatc cactttcttc
     3421 tgccggagct tcatcataag catcagcgca gccatctgct tatagcgacg gataccctca
     3481 tcatcaagaa gaccgacatc aatcaccctg ggtggcctgc cttctccata aagctcccga
     3541 ccaaacgccg gatcatccag gcagtcaggc cagttcatgc tgtatggata aggactgggt
     3601 acaccatggt aaaaaagcac cggtatcaca acgggagcac acgcatgtgc tttcaggtgg
     3661 cgatgtatca ccgccagaac atagcgcatc attcgcaggg ccatatactt atccgccttg
     3721 ctctggtgct ctgtcagcgt ataaatgtat ccatccctgc catctgtggt tttcatactc
     3781 cacagaacat cacaggcaaa ctggcgaaga tccggatcca caaacgtggt cggctccagt
     3841 tgcagggtgt tcatatcgca ccggttcagt tgttccgggg gcaacgcgcc tttcaggaaa
     3901 tccctcgcaa cagagggaca ttccatcatc gctctgaacg ccgcatcatg tggcgtgggg
     3961 gtagtgggtg tgttttcatt cacaaccgcc tccttgaatt aaaagtgggg cggtaagccc
     4021 cgtggttaac atcagatgac atggataatc cgtcgttcaa cgccataccc acgccccata
     4081 aaggggatat catcgtcaaa gtccatcggc ggcattcccc cgcttcctga ggaagcctga
     4141 ccgttttttc ccgttgattg ttgtgcattt tgtggtggtt gtccccatcc tccctgagca
     4201 ggagcagatg aaacatttcc accaccctgc tgctgacgcg gagaccccag catctgcagg
     4261 gtaccattga cactcacaac aacttccgtg gaataacgat caacaccatt ctgctgatcg
     4321 aaccacttcc gggttcttaa ctgaccttca atatacacct gtgaaccttt acgaaggtat
     4381 tcaccggcaa tctcagccag ttttccgtaa atcacaacac ggtgccattc agtgtgttcc
     4441 ttcatttcac cgctctgttt atcacgccag gtatcagtgg tggccagcgt cagatttgcc
     4501 actgcacctc cgccgttggg gaaataacgg atttccggat cctgtcccag atgaccaatc
     4561 agtatgactt tattcactcc acgcgatgcc ataacaactc cttatcctca cacacattca
     4621 cgctgagtcc agatgccgtt ttccagagcc cggactgcct gctcataatt tctgaaatac
     4681 tcttcggatt cccgggacac aggcccttcg tcgcactgag taccaatata ataaccacga
     4741 gcactgctga ggatctgcag aggtaaggta tagcccgctt tggcggccag aattccgctt
     4801 gtcggtttca taacatgttt ccttaaagtt aaacgataag aaaacagacc tccctgatgg
     4861 ttggtctgtt ttcatcaggg aggttgagtt gaaaaatacg atcagaaaga attttctgcg
     4921 tattcccgcg gggtattgcc attctgtggc ggtatggatt ccgatttttc agtcttataa
     4981 accatgtcct ggcctatttt gatccactcc accttgatca ggcgggcttt cagactgacg
     5041 cgttgctcac cggcatgttc accccggtta agggtaaaaa tgtcagtaga ggggttgctc
     5101 atggtaaaac caatcagcac cttgcgatct tcatccaccg ctttctggca gcgggcaatc
     5161 agggaagttg cctctttacc ggcaaccgtc acatcaaaac gcacatagga cgggctgtcc
     5221 gtcggaccac tcagtgcatt cacaacacaa ctgagaaatg tcccgttctg atgattaacc
     5281 tgacgaatgt tgctgagata ccccaggcct ttcagtgtca gattgaagta ctcagtttta
     5341 gcgtttgcag aagaagtatt tgcagtcata atgatatctc cattctcagt atcggaaaga
     5401 cagagaacat catcatccct gacgggttgt gatattcccg tcagggagcc ggaacagaac
     5461 tattccggct gactcagtaa cagttaaag
//
LOCUS       DAZUCC010000267         5486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZUCC010000267 DAZUCC010000000
VERSION     DAZUCC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.269"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..266)
                     /locus_tag="ACHY09_004593"
     CDS             complement(<1..266)
                     /locus_tag="ACHY09_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS3887463.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGG"
     gene            complement(263..1933)
                     /gene="menD"
                     /locus_tag="ACHY09_004594"
     CDS             complement(263..1933)
                     /gene="menD"
                     /locus_tag="ACHY09_004594"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFS3887464.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2022..3317)
                     /gene="menF"
                     /locus_tag="ACHY09_004595"
     CDS             complement(2022..3317)
                     /gene="menF"
                     /locus_tag="ACHY09_004595"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFS3887465.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGEIAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3396..3701)
                     /gene="elaB"
                     /locus_tag="ACHY09_004596"
     CDS             complement(3396..3701)
                     /gene="elaB"
                     /locus_tag="ACHY09_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFS3887466.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3756..4217)
                     /gene="elaA"
                     /locus_tag="ACHY09_004597"
     CDS             complement(3756..4217)
                     /gene="elaA"
                     /locus_tag="ACHY09_004597"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3887467.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            4282..5199
                     /gene="rbn"
                     /locus_tag="ACHY09_004598"
     CDS             4282..5199
                     /gene="rbn"
                     /locus_tag="ACHY09_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFS3887468.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCNA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPVTELANDFTVFNV"
     gene            5387..>5486
                     /locus_tag="ACHY09_004599"
     CDS             5387..>5486
                     /locus_tag="ACHY09_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887469.1"
                     /translation="MMVTVVSNYCQLSQTQLCQTFAEKFTVTEELLQ"
BASE COUNT         1302 a         1512 c         1360 g         1312 t
ORIGIN      
        1 ccgccaagcg agtaccccac cagccagaag ttaaggatgt tgtaactaac taaggtttta
       61 cgcagtaagt cggtgacatc atcaaatcca tcgacgctaa tcgccgccga accaccgtga
      121 cctgggagat caacatacaa ccgtgagtag tcggcaaacg cctcgcccac ttcttgccat
      181 tcatggcaat cgccggaaaa accgtgcaaa aacaccagcc agggtaaacc tggtttaccg
      241 tgttttgcct gcgcgtgcag gatcataaat ggcttacctg cgccagcagt tgctggaggg
      301 tttgcgcacc atcggtgtcg ttaaccacca tttcaattac cgtggtggtc ggcgtgcgcc
      361 aggcgtcggc aagtgccgtt tcaagttcct gccagttttg cggacgatga tatttcagct
      421 cgaacatcgc ggcggcgtgc tcaaaatgga cgttttgcgg catcagatag aaacgctcac
      481 gctcgttttt cggcgttggc aacagcgaga aaatttgccc gccgttgttg ttcaccacaa
      541 ttaataccag cggcgcggaa acctgacgta ataacgccag cgcgttgaga tcgtaaagtg
      601 ctgagagatc gcccacaatc gccagcgtcg gtttgccact ggcccgctga acgccagcgg
      661 cggtcgagag cagcccgtcg ataccgctgg caccacggtt gctgtacacc gggtaacctg
      721 ccggaagttg cgaaagcgca tcaatcagac gtaccaccag gctgttacca acaaacaatt
      781 gaccctgttc aggcagatag tcgctgatgc gatgcgccag ttgcgcttcg ccaaacgcat
      841 cgcgacgggc aataaccgcc tgcattgcct gttccgccag gcgcggaatt tcaacgcacc
      901 agggctggcg tttttctgcc ggatgctgct ccagccagtc ggcaatattg gcaattaagc
      961 gacgtccacg atggtgtgcc ggatcaagtc gcccttcgat gtcatcaaca atccagtact
     1021 cttccggttc acagcttgcc tgccattgca ggagccgttt gcccgtcagg ctgcttccca
     1081 gttgcaccac aatttgtgcc tgttgcagtt cgctggtcgc tttggcattg cctaaccaga
     1141 gatcggcaca cggcagcggc tgtccggttt gcgacagcac gtcgccaatc agcggccagc
     1201 caagagtttg cgcccacagt gcaacttttt tgccctcttc cgcactcatg cgcccggcaa
     1261 ccaccacgcc gcgcttttgc cgccagaaga accagtcgcg ctgtttttca ctttccagac
     1321 gaggcgcttc acgcagccac ggtttgtcgt cctgccacca gtcgcccagc cgctgttgcc
     1381 agctaagccc ggtgtcgtcc atttcgccat acagcggttc agcaaacggg cagttgatat
     1441 ggactccgcc cgcatgaagc gtaccgagag catggtcgat ggtagaaacc agccaacgtg
     1501 caggaatatc ctgagtcggg cgcggcagtg aaatactgtg cgtggggtga gaggcgaaca
     1561 ttcccggctg acgaatcgcc tgattcgcgc cgcagtcaat tagctccggc gggcgatcgg
     1621 cggttaacag gatcagtttt tctccggtta acccagcttc aatcagtgcc ggatagagat
     1681 ttgctaccgc cgtgccggag gtcacaatca ccgccaccgg ctgcttgctg actttcgcca
     1741 gccccagcgc caaatgcccc agtccacgct catcgaaatg ggtgtggtga atgaatgcgg
     1801 aattctccgc cgccgctaac gttaacggtg tagaacgcga gccaggggcg atacagatgt
     1861 gtctgacgcc gtgacgcgtt aatgcttcca gaatgaccgc cgcccagcgt cggttaaatg
     1921 cgcttactga catgagtttg tccggtatca atattgcggc taagtataag gagctacaaa
     1981 aatagattat tgatatgaat cggtaatgat gcgactcatt actattccat ttgtaataaa
     2041 gtacgcagcc ctgccgcttt gttatcgatt tcctgccact cttgctcggg gtcagaacca
     2101 cggacaatgc ccgcgccagc atatagccgc acgacattgc cactgatttt tgctgagcgc
     2161 agggaaacgc agaattcgct ttgttgtaaa gagagatagc ccgcagaacc ggcgtaccat
     2221 tctcgggtga acggttcgtg acgggcgata aactgtcgcg ccagatcgcg cggtaagcca
     2281 gcaactgctg ccgttggctg caactgatgt aaacagatca catcatccgc tttgttgagt
     2341 gcagtccaga tacagcggcg aagatgctgc actttacgca gacgcagtac ctgcggcggt
     2401 aaaacatcca acgtctgggt atcggcctgc aatcgttggc agatatcctc caccaccaac
     2461 atgttctcgc gctggttttt atcatccgcc atcagccact cacctaactg ctgcgcctgc
     2521 ttatcatcag gatgatttgc taccgttccc gccagcgctt cagtacgcag cgctttgtca
     2581 cgccgccgcc ataaccgttc cggtgaagag ccaagaaaag caatttcgcc atcaaaggcc
     2641 atgtaaaaat ggtagcaatt cagattcagt cgacgactgg cagccatcat cgccgccgca
     2701 ttgaccggac ttgcgaaatg caggtcagtt gcccgagcaa gcaccacttt gtcgagctca
     2761 ccttcggcga tggttttcgt tgccagttcg attaattgcg tccagcccgt tttgtccggc
     2821 cagtgttgtt ctcgggtggt ggttaaatgt aacccaggca agggtttaat actcaccagt
     2881 gtggcgataa attcttttgc cttaatcgca tcatgctgaa gggagctttc gctgaatagc
     2941 gtcagccgca gcgtggcttt accgccacag cgtcgccatt cgaggcgtgg taaaagtaaa
     3001 ttgccctgcg acgggtcaaa cgcatttagc ccccagatgc gtaagtcggc gtgttccggg
     3061 tgctgacgaa gaaacgtttg tgcctggtcc aacgacgtaa aacgggtaat cgcgcccagg
     3121 acggcagctt cttcatcacc attacgttgt tgccagtaga attgcgggta cgtttgctga
     3181 ctggccagcc agctcaaggc atcaaaagcg tctttgagag ggaaaggaat atcaataacc
     3241 cgaatgccgg gtgtcgccgg aatctcttgc gacaaatggc gcagtagatt ttccagcgcc
     3301 gtagtaagtg attgcacgcg gacctctccc tgttaaaagc ctcacattat acggggtacg
     3361 acaaaaaaaa tgcagtaccc cggtgtaggg aggttttaac ggcgtgccag caacagtcct
     3421 agtaccagcc caacggccgc gcccacacca attccttgcc agggtttttc gtggacgtag
     3481 tcatcagcac gataaacagc ctgcttcgcc cgatagtaat aactgtctga agcctggcta
     3541 acccgttttt tcacatcatc cagcgctttt tctgcacgcg ctttcagctc aacatatttc
     3601 tgatcggcgg gatcgccaga ggagcggagc acctcttcca gtgtttcact aagcagcgtc
     3661 aggtcatcat caatacgtgt atcaccaaac tgattagaca ttctcgttct ccatttgtgt
     3721 aaaacctgtc actaactata gacaagggtt attgattacg cctgaattac ctcgcgtgcc
     3781 atcccaatgt gtgggatacc atcttcttca taaatatcgg tcacagggat gaaaccaaat
     3841 tgcccataaa agttttgtaa atgcgcctgc gcccccagat aaatcggctt ctccggccag
     3901 cggcgagtac acgattccag cgccttactc atcaattgct gaccgatttt ttcaccgcgt
     3961 aaggcttcgc tgacaatcac ccgaccaatc acaacgggtt gcagttcgtc gtcacttttc
     4021 agaatcctcg catacgccac cagtgtcccg ttatgccaac cgagaatgtg gcgattttct
     4081 ccctccagat cgtcaccatc gatatcctgg taaggacagt tttgttccac tacaaatacc
     4141 gcacaacgca gttgtaataa ggcataaagc tgagaaacgg ataaatcaga atgatgcaga
     4201 tcttgccagt cgatcatgga aggctccttt gtgctgtttg ctttatacta gccggaatat
     4261 tttcagcagc gtgatgaact catggaatta atttttttag gtacttcagc cggtgtgcca
     4321 acccgtacgc gcaatgtcac ggcaatatta cttaacctgc aacatccgac ccagagcggt
     4381 ctttggttgt ttgactgcgg tgaaggcacc cagcatcagc tactgcatac cgcctttaac
     4441 cctggaaaac tggacaagat ttttatcagt caccttcatg gcgatcatct ctttggttta
     4501 cccggcttgc tgtgcagtcg ttctatgtca ggcattatcc aacccttaac gatttatggt
     4561 ccgcacggta tccgtgaatt tgtggagacc gcgctgcgga ttagcggctc atggaccgat
     4621 tatccgctgg aaattgtcga aattggcgct ggcgaaattt tcgatgatgg cctgcgcaaa
     4681 gtgaccgctt atccgctgga acacccactg gaatgctatg gctatcgtat tgaagaacat
     4741 gataaaccgg gtgcattaaa tgctcaggca ttaaaagctg ctggcgtgcc gcctggccca
     4801 ctgtttcagg cattaaaagc gggcaaaaca atcacgctgg aagacggaag gcagattaac
     4861 ggcgcagatt acttagccgc tccagtgcca ggtaaagcgc tcgctatttt cggcgatacc
     4921 ggcccctgca atgccgcact tgacctggct aaaggtgtcg atgtcatggt gcacgaagcg
     4981 acgctggata taaccatgga agccaaagcc aatagtcgcg gtcatagctc tacacgccag
     5041 gctgcgacac tggcccgtga ggctggagtc ggtaagctaa tcattaccca cgtcagctcg
     5101 cgctatgatg acaaagggtg tcagcacctg ttacgtgaat gcaggtcaat tttcccggtg
     5161 actgaactag cgaatgattt caccgtgttt aacgtttaac tctgcttatt attaaaaaca
     5221 gggcgaaact tgccctgtta tcgcaacccg cgctacatat atccacccac acgggtatat
     5281 tgagcgattt ttgatattta ttcatctcct cacgcactat attcagagca ataagttgcc
     5341 tctttttatc ttcattcagc acaacctttg caactaaaag gagcctatga tggttacagt
     5401 tgtcagcaat tattgtcaat tatctcaaac ccaactctgt cagacatttg cagaaaaatt
     5461 tactgtgacc gaggaattac tgcagt
//
LOCUS       DAZUCC010000268         5444 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZUCC010000268 DAZUCC010000000
VERSION     DAZUCC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.270"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(182..1555)
                     /gene="cysG"
                     /locus_tag="ACHY09_004600"
     CDS             complement(182..1555)
                     /gene="cysG"
                     /locus_tag="ACHY09_004600"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFS3887470.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGQVVGLRDK
                     LNWFSNH"
     gene            complement(1574..2380)
                     /gene="nirC"
                     /locus_tag="ACHY09_004601"
     CDS             complement(1574..2380)
                     /gene="nirC"
                     /locus_tag="ACHY09_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFS3887471.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSIRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            complement(2507..2833)
                     /gene="nirD"
                     /locus_tag="ACHY09_004602"
     CDS             complement(2507..2833)
                     /gene="nirD"
                     /locus_tag="ACHY09_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFS3887472.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2830..5373)
                     /gene="nirB"
                     /locus_tag="ACHY09_004603"
     CDS             complement(2830..5373)
                     /gene="nirB"
                     /locus_tag="ACHY09_004603"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFS3887473.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHANLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
BASE COUNT         1283 a         1493 c         1391 g         1277 t
ORIGIN      
        1 aagaacgata caggaatatt taatcggcgt cagtgagtga atgacttaat tttgtaagtc
       61 gctgtaacgt tgaatataac ccattagcag ataatattta ttaaggggag tgtttttatc
      121 tgctgaatat ataaagagag ttgtaaacga aaaaggccag aattctctgg ccttggttaa
      181 attaatggtt ggagaaccag ttcaatttat cgcgcaggcc aacaacctga ccaataataa
      241 ttagcgatgg actgttcatt tgctgcgcca gttcgcccag ttgtgtgagc gtaccgtcaa
      301 tcacgcgctg cgtgactgct gttccgtttt cgacaattgc caccggcatt tcgccaggca
      361 taccgtgttc aatcagcttt tgctgaatag tcgcggcctg attcaacccc atatagaaca
      421 ccagcgtctg tttttctgcc gccaggtttt cccagtccag ttcgccaccg gtttttaagt
      481 gtccggtaat taagcgtacg ctctgggcat aatcgcgatg cgtgagcgga atacccgaat
      541 aggcagagca accagaagct gcggtaatac ccggaaccac cgagaacgga atacccgcgt
      601 tgcacagtgt ttccagctct tcgccaccac ggccaaaaat aaacggatcg ccgcctttca
      661 gccgcaccac gcgtttgcct ttttgcgctt cccgtagcag gatctggttt atctcttcct
      721 gcggtacgca gtggtatccc gcgcgtttgc cgacgaaaac gcgatcagca tcgcggcgta
      781 ccagattcat aatatcgtca gaaaccagac ggtcgtagac caccacatct gcctgctgaa
      841 tttgttgcag acctttcagt gtcagcagcc cggcatcgcc cggacctgca ccaaccagca
      901 ccacttcacc gcgatggtcg agcggttcgt tgattaactg ttcggtggtt tcagtaatgg
      961 ctttctgatc gttgtttgcc agcgactgcg ccaggcggtc gttaacgaac aatttctccc
     1021 agaaacggcg acgctcaccc atcgtggcga actgttgttt cactcgcccg cgtaattgcc
     1081 cggcgtattt cgctacctgg cccagatgca gcggcagcag tgattcgagt ttttcgcgca
     1141 acaggcgcgc cagaaccgga gaggtgccgc cagaggagac cgcgaccatt agcggtgagc
     1201 ggtcaataat tgacggcata ataaagctgg cggctttcgg cgcatcgacc acgttacaga
     1261 agatgcgacg agcttcagcg gcttcactga cgcgctggtt aagcgcgtca tcatccgtcg
     1321 cagcaatcgc cagccagcag gtgtcgagaa ggctttcatc aaatggccct tcgacgaggg
     1381 ttaacatgcc tgcatctgcc catgcggtga actgtggaat aaacgctaat gcattcaccg
     1441 ttaagcgagc gcctgcgtct aacagtaacc ttgctttgcg ttccgcgaca tcaccaccgc
     1501 cgacaatcag acagtcgcga tcgcgtaatt ggcaaaatat aggcaaatga tccacgtaaa
     1561 aaccccttag taattaaccg gcagctgttt cagtttgatt aaatttgtcc gcaaccggac
     1621 gattcgcttt cggcgtagca taccaataac ccaatcccat gaatacggca cctgataaag
     1681 tattacccag cgtcacccac agcaggttat gaccaatacc cgccagcgtg taggcttcgc
     1741 tgtggttgcc gaaccaggag agggcgaaca gcgtcatgtt agcgatggag tgctcgtagc
     1801 cggacgcgat aaatgccagc agacaccacc agatagcgat aaatttcgcc gccccttcag
     1861 tgcgcagcgc catccagata gccaggcaaa ccagccagtt acacaatgca cctttgaaga
     1921 acagtaccat tgccggtgca gtggtttttg ccagcgcgac ggagtgaacg atgctggtat
     1981 ctaccggcag caggctaccg ccgccccagc tatagagcat ggcaacgaag acggaaccga
     2041 ccagattacc cagccaggtt tgcggcagga ttgcccacat ttgcccgtgg ctgatggtgc
     2101 ccgctttcac cccaaaggtg aggaacatgg tgtgtccggt gaacagttca gaaccggcga
     2161 taatcaccag cgttaaggcg ataccaaagg tcgcgcccat caccagagga cgtatggacg
     2221 gatcgagcaa attaccgagc gtgaaaatca ggatgatccc aagacccaca tacgcgcccg
     2281 ccatggcgga gctgacccaa aagccgagcg ggttatttgc cgacaggcgt gcaatgcgcg
     2341 cagcgttagc cgcacactta ttaatagtgt ctgtaaacat ttgattatcc tttaaaaaat
     2401 aaaaacaaaa aaataaaaca aaaagtttaa aaaatgtcct gcaatatacc gaaggtcatt
     2461 acaggaccat gcaaaaaggg gaggcattgc gcctcccgtt aaaacattaa ccgcgcagct
     2521 gcaccacgcc gtctttcact cgtgcttcgt aatgtttgac ggaaaactgt tcgtcttcca
     2581 tgcacaagcc atcacttaag cgaaaacgct gttttttcag cgggctggcg acccacagct
     2641 cgccctggtg ttccgcaatc agcccgcgtg acagcacgct ggactcgaag aacggatcga
     2701 tgttgctgat cgcaaacacc tgatcgctgt gatacgggcg gaaaattgct acttgttcgt
     2761 cacctaacag cgcgcagacg ccggtttcag gcaggatgtc atcgattttg cagatgtctt
     2821 tccactggct catgcgttgt cctccaccag agttaccggg atacgttcat acggcgttgc
     2881 cggacggtgc tgttcgcgct ctggcaccat ctgcacgttc gggtcacgct tatcgctgtt
     2941 gatgaagtgt ttgaagcgag tctgcgcaga cggggtattg accgtttcag tccactcaca
     3001 cactaccgct tcacgcaggc gcgccatctc ttcttccaga tgtgcgttca gccccagttt
     3061 gtcgtcaatg atcaccgctt tcaggtaatc gatgccacct tcgaggtttt ctaaccacgg
     3121 cgcggtacgc gtcagtttgt cggcggtacg gatgtagaac atcatgaagc ggtcgagata
     3181 tttgatcagc gtttcgcgat cgatatccgc tgccaacaga ttcgcatgac gcggtttcat
     3241 gccgccgtta ccgcaaacat acaggttcca gcctttttcg gtggcgataa tacccacgtc
     3301 tttaccctga gcttctgaac attcacgggt acagccggag acaccgaact tcattttgtg
     3361 cggagtccgg atacctttgt agcggttttc cagttccacg ccgaggccga cgctgtcgcc
     3421 cacgccgtag cggcaccagg tgctacccac gcaggttttc gccatacgca gtgctttcgc
     3481 ataggcatga ccggtttcga agccggcttc aatcagctga cgccagatct ccggcagatc
     3541 gtctttctgt gcgccaaaca tcgccaggcg ctgggagccg gtgattttgg tgtagagatt
     3601 aaattcacgc gcgatacgac ctactgccat cagcccttcc ggggtgattt caccgcccgg
     3661 agaacgcggg atcaccgagt aggtgccgtc tttctggatg ttagcgagga agttatcgtt
     3721 agaatcctgc agcggagtat gttccggttt cagaatgtat tcgttccagc aggaggccag
     3781 cagcgaaccg acggtcggtt tacaaacttc acaaccgtag cctttgccgt gtttcgccag
     3841 cagttcttcg aaggttttaa tgccttcaac gcggatcaaa tggaacagtt cctgacgcga
     3901 ataagcaaag tgttcgcaca ggttgttgtt aacttcgatg ccctgtttcg ccagttccgc
     3961 gttcagtacc tgagtgacca gcgggataca gccaccgcag ccagtacccg ctttggtttc
     4021 agctttcagc gccgcaacgg tgtggcagcc tttgttgatg gcagcaatca gatcaccttt
     4081 ggtgacgtcg aagcaggagc agatttgcgc gctgtccggc agtttatcaa caccgataga
     4141 cggcttaccg ctacccgagt gcgccggcag aatcagagaa tccgggtttt ccggcagctc
     4201 gatagcattc agcaccagtt gcagcaggtt gccgtagtcg ctggtatcgc ccaccagtac
     4261 cgcaccgagc agggttttgt tgtcttcgct gacaataagg cgtttgtaga tctctttgct
     4321 ttcgtcgagg taaacgtagc tacgtgcgcc aggtgtgcga ccgtgcgcat caccaatacc
     4381 gcctacgtct acgcccagca gtttcagctt ggcgctaagg tctgcacctt caaaggcgtt
     4441 ttcgctacca agaatatggt caacggcgac ctgcgccatt ttgtagcctg gtgctaccag
     4501 accaaataca cggttgttcc agcttgcgca ttcaccgatg gcgtagatat ccggatcgga
     4561 agtctggcag gaatcattta tgacgatacc cccacgcgga gcaacgtcca gaccacactg
     4621 ggttgccagc ttatcgcgcg gacggatacc ggtagagaag acgataaagt cgacttccag
     4681 ttcgctgccg tcggcaaaac gcatggtttt acgcgcttca acaccttcct gcacaatctc
     4741 aagagtgttt ttgctggtgt gaacgcgcac gcccatactt tcgattttgc gacgcagctg
     4801 ctcgccgccc atctgatcaa gctgttccgc catcagcata ggggcaaatt cgataacgtg
     4861 ggtttcaatg cctaagtttt tcagcgcgcc agcagcttcc agacctaaca ggccgccacc
     4921 aacaacggca ccgcgcttgc tgcgacgagc gcaggattca atggcgttga ggtcttcaat
     4981 ggtgcgatag acaaagcagt cctgagtatc agaaccttta attggcggga tccacgggta
     5041 ggaaccggtc gccatgatca gcttgtcata aaaaacggta cgtccggcgc tggagtgaat
     5101 caccttctcc tgacggttga tggtgatagc gcgttcgccg accagaactt tgatgccgtg
     5161 tttctcgtag aagccttcgc gcaccagcga cagctcttcg gcggtgtggt gagagaagta
     5221 agacgagagg tgtacgcggt cataagcgat gcgcggttct tcacagaaaa cggtaatatc
     5281 aaagttggcc gcatcagatt tatcaagaag atcttcgata aagcgatggc cgaccatacc
     5341 gttaccgata attgcgagtc tgactttgct catttttgcc tcgatttctt ttctattacc
     5401 gcctacctta acgattcagc aaccccgctt attgatgtaa atca
//
LOCUS       DAZUCC010000269         5436 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZUCC010000269 DAZUCC010000000
VERSION     DAZUCC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.271"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            290..475
                     /locus_tag="ACHY09_004604"
     CDS             290..475
                     /locus_tag="ACHY09_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887474.1"
                     /translation="MFISCVPSKATPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISTLKGFSTSF"
     gene            489..1049
                     /locus_tag="ACHY09_004605"
     CDS             489..1049
                     /locus_tag="ACHY09_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS3887475.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            1149..3188
                     /locus_tag="ACHY09_004606"
     CDS             1149..3188
                     /locus_tag="ACHY09_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFS3887476.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENGVNVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSAWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMALYWPVSSLPQGVTLTGDA
                     GNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     IPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            3335..3517
                     /locus_tag="ACHY09_004607"
     CDS             3335..3517
                     /locus_tag="ACHY09_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFS3887477.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            3553..3798
                     /locus_tag="ACHY09_004608"
     CDS             3553..3798
                     /locus_tag="ACHY09_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFS3887478.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENDPQPVEQPEPVQPGTDGTMM"
     gene            complement(3837..4303)
                     /locus_tag="ACHY09_004609"
                     /pseudo
     CDS             complement(3837..4303)
                     /locus_tag="ACHY09_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4418..4618
                     /locus_tag="ACHY09_004610"
     CDS             4418..4618
                     /locus_tag="ACHY09_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS3887479.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPNHQHA"
     gene            4572..5309
                     /locus_tag="ACHY09_004611"
     CDS             4572..5309
                     /locus_tag="ACHY09_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFS3887480.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESIFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRANVHKFRQFRYIRFLNKRAK
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
BASE COUNT         1488 a         1240 c         1360 g         1348 t
ORIGIN      
        1 tctgaaataa cgcatatttc agatgctggc tgctgtgtgc tatgcccccg ttaattaaat
       61 acttgcagcg gatacccaat gcccattaat acaatattgt atctgtctgt aataaacacg
      121 gtcatcacca ccacgcattt cccagtctcc gtcagtctta aaaccggtaa tcacatttcc
      181 tgatgacagg gagtaattat attccagtcc gccgcttaat gcctgcgctc ccagtcttac
      241 ccctgtaaca taacgctgat tggccgttga ttccggcatt cctgacgtaa tgtttatatc
      301 ctgcgtgccg tcaaaggcga caccattgat tctgcgggca tttgccagcc tgcttgctgc
      361 aacagccgta ccgtttgatg gcagggcatt cgcggccctg tttaccgtat cccgtaaacc
      421 gaggtttcag ggatgccgta tatctacttt aaaaggcttt tccacctctt tttaaggaag
      481 gtaaatacat gctgattggc tatatacgcg tatcaacaaa tgaccagaac accggtttac
      541 aaagaaatgc gctggaatgt gcaggatgtg agcagatttt tgaagacaaa atgagtggta
      601 aaacgacaca gcggccggga ctgaagcggg cactgaagaa actaaaaccc ggtgacacgc
      661 tgatggtctg gaaactggac aggctggggc gcagtatgcg ccaccttgtt gcgctgacag
      721 atgaattaca gcaacagggg ataaattttc gtagtcttac tgacaacatt gatacgtcaa
      781 cccccatggg gcgcttcttt tttcacataa tgggtgcgct ggcagaaatg gagcgtgaat
      841 taattgtcga acggacacgg gctggtcttg cagctgcccg tgaaagggga cgaaccggcg
      901 gcagaccccg ccgtctcacg caggaacaat acgagcaggt cgggcgttta ctggccgggg
      961 gtgtcagtcg aagacaggtg gcacttattt ttgatatcgg tctttcaacc ctttatcgtt
     1021 attatcctgc gagcgtttcg gaaaaataag acgctctctt tgttgggcct ttactgaatt
     1081 aacgcaaaat ggcgtttatc ggtctgtgga tatgataatt cagtttattc aggaacaggc
     1141 tgattattat gacgtccaaa tgggttcaac tatcatcaat gcccggaaac tttactgtta
     1201 aagtttccgg tggtacagcg gcatttcttg aggctccctt tcctccggcc gaaacaaaag
     1261 gaggaatgac atttgctgac tgtctcatca gttttaatac acgggattgc ctgtgggtaa
     1321 ggccagtatc cggtgatccg agtgtggaaa ttaccggagc gggtattggt gcggtcattc
     1381 cgttaagtgc tgatgttgcc ggtactgctg aaccgtcaga ctgggataat gctgaaacac
     1441 atacccgtcc gtcaggaaat gaaactgctt ccagctcccc ttcctggtat tacgtggtgg
     1501 ttcttgctgg tcagtcaaac ggtatggctt acggtgaagg tctgccactg ccggagacat
     1561 atgaccgccc cgaaccgcgt attatgcagt tagcccgtcg cagtacagtc acgccgggag
     1621 gtaaagcctg tcagtataac gacatcattc tggccgacca ctgtctgcat gatgtgcagg
     1681 atatgagtgg aaaaaatcac ccgaaagcgg atgtggctaa agggcaatat ggcaccgtgg
     1741 gtcaggggct tcacattgcc aaaaaactgc tgccgtttat tccggcagat gcggggattc
     1801 tgctggttcc ctgctgccgt ggtggttctg cattcacggc gggtgctgac ggcacgtaca
     1861 gtgacagcac gggtgcttca gaggactcag cccgctgggg tgtggataag ccgctgtata
     1921 aggacttaat cagccggaca aaagcggcac tggcgaagaa cccgaaaaac cgcctgcttg
     1981 ctgtggtgtg gatgcagggc gagtttgata ttgatgcgaa gccgacggag cattctgcgc
     2041 tgtttctggc gatggtggaa aaattccgcg cagacctggc tgaacaggcg gaacagtgta
     2101 ccggtggcag tgctgctggc gttccgtgga tttgtggcga taccacgtat ttctggaaac
     2161 agaaaaatga accagcatac caggcaatct atggcggcta caaaaacaaa acagataaaa
     2221 acattcattt cgttccatta atgacagatg aaaatggcgt gaatgtaccc accaacaatc
     2281 cggcagaaga cccggatatt gaatccattg gttattacgg ctcaacgtgg cgtaacagtg
     2341 ccgctacctg gacatctgcg gaccgggcca gccatttcag tgcctgggcg cgccggggta
     2401 ttatttctga ccgtctggcc acggctgttc tgactcatgc agggcgaacc acagtaaaag
     2461 ccgatgttcc atcttctgaa actgaagcac cagtgccgtc accttcagaa actgaagcag
     2521 taaccacaac actgctgtct taccgtgtca gtgagtcaga aggaaatctg aaagcccagg
     2581 gctgggagcc ggctggcggt aaagctgaaa ttatcagtga tgcaggaggc acaggtggta
     2641 aggcaatgaa actgaccaag gaaacaggta aatcatcctg gtatctggat catgatgccg
     2701 gtactggtgc tgaactgctg aaaaatggtg gtttaatcag ttgtcgtttt aaagtacctg
     2761 gcgacctggt ggcaaaccag tatgtcatgg cgctttactg gccggtttcc tctctgccgc
     2821 agggtgtcac cctgacaggg gatgcaggga ataatctgct ggcatcgttc tacatccaga
     2881 cagacgcaaa agacctgaat gtgatgtacc acaatgcaaa agtggcaaca aataaccaga
     2941 aactgggaac ctttggtgca tttgataatg aatggcatac gctggccttc cgttttgcag
     3001 gaaataacag ccttcaggtt attccggtta ttgatggtca ggatggtgcc gcgtttaccc
     3061 tgacgcagtc accagtagga actttcccgg tggacaaact ccgcgtaacg gatatcacca
     3121 aaaatgcaac ttacccggtg ctgattgaca gcattgtggt ggaagtgaaa aaagcggtaa
     3181 cagaataaaa aaatcccgcc ggtcatgata tggctggcgg gagctaccta acccataaag
     3241 gaaaataaaa atactcccga aggagtggtt atcagtcgct tcgatgtttt tacccgataa
     3301 cgaattatgt agtcaatatc aaaggtaagg ttatatgaca ttcgttcata caatgctgct
     3361 ttatttctgc gctgtggtca gtgcgttata tctggtgagc ggcggttata aggtcatcag
     3421 aaattatatt cgccgtaaaa ttgatgatgc ggcagctgaa aaactcagta aaacagcaca
     3481 ggcaccgtca tccccaaacg acccgacccc gctctgataa cggggcaata tacctgcaaa
     3541 aggagaatat tcatgccaga gattaaaggc acggttactg aagagcttgt caaacaggca
     3601 ctttattctg aagaagtgaa ccgcgtgctg aaggcgcagg ttcgcaagga ttttgaggca
     3661 caaatcgacg catatgttga tgaagtgctg gcccgaatgg ttggccgttc tccggctgaa
     3721 aacagcacgg aaaatgatcc tcaacccgta gagcaaccag agccggttca gcccggaact
     3781 gacggtacta tgatgtaaca ataccgaccg gcaggtttcc ctgccgggtg agtttgttaa
     3841 tgcacgtttt gtgtattttc cctcctggga gaacaggcag gacatatcag catcctgaaa
     3901 caggaagcat ttttgggttt gtatgtcttg ccattttgag atcgagaacg gcgatagatg
     3961 agctgtaagg gagtcttaca ggcagggcaa acaggctctt tcatccatga taaagatctc
     4021 ctttttatgc atctctgctt tacaggagac tggggaatat cataggatac gccacgagct
     4081 cgaagtaaag cattgatgca aaattgtcta aatcggccca gaatagttcg cgctatctgg
     4141 gtcgctcttt atgtatgtag tatcccattg atttgaaaca tactacatat acgcgcgatc
     4201 ctaccttcac tatataaaaa gtcaataaaa tgatcatttt atgatcgttt tcaccaccta
     4261 actcttcgtt ttctttaagt aaataatcac ctacctgaat cattgttgat gacttttcaa
     4321 tctatttgtt gtatcaatta aacaaatcga atcgatcgtt tttgtcgatc gattgcatga
     4381 gaaaggcatc aacaacaaag caggaggtta tgcggccatg tatcgaaaca tacggtgccg
     4441 acactgcaat aagctactgg cacgggccag tttcagttat ctggaagtaa aatgtccgcg
     4501 ctgtaaaacc ctcaatcaaa tcacatctcc gagcgccaca gagcacccca catacacaag
     4561 gaaatcctgt cgtggggaac agaaagcaag tcacatcccg aatcatcagc acgcctgaac
     4621 ttattcgcta taacgacaat atcgttggtt atggctcccg tgagttgcgg gttgagacaa
     4681 taagttgctg gctggccaga ctggtcatcg tcaataaaca ttacagccac cgtttcgtaa
     4741 ataactccta ccttcatctg ggcatatttt cagaacggga acttgttggc gtaatgcaat
     4801 ggggttacgc ccttaaccca aacagtggtg cacgcgtcgt aacgggtacg cagaaccgag
     4861 aatatatgga gcttaaccgg ctatggctgc atgactgtat gccgagaaac tccgaatcaa
     4921 gggcaatcag ttacgcgctc aagctaatca ggcagcttta tccgcaggta cagtgggttc
     4981 agtcgtttgc agatgagcgt tgtggctgtc ttggtgtcgt gtatcaggca agcaattttg
     5041 attatgtagg cagccatgaa tcaatattct atgaacttga tggcgagtgg tatcacgaga
     5101 tttgcaggaa tgccattaaa cgaggcggac aacgaggtga acatctgagg gctaatattg
     5161 acagagccaa tgttcataag ttccgacagt ttcgttacat ccggtttctg aacaagaggg
     5221 ccaaaaaacg cctcaacaca aagctattca aagtccagcc ttacccaaaa ccacaaacag
     5281 ttaaaactgg tttgaaagag agtgaatgag cgcttaaaaa ctcattctaa aactcaaaag
     5341 aaaagcgccg atttagtatc aaataagttg cgtatcggcg tttttcagca gtatcaaatg
     5401 aaatgcaaat cggtatcaaa aaaatcgccg cgctat
//
LOCUS       DAZUCC010000270         5412 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZUCC010000270 DAZUCC010000000
VERSION     DAZUCC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.272"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            126..1484
                     /gene="mltD"
                     /locus_tag="ACHY09_004612"
     CDS             126..1484
                     /gene="mltD"
                     /locus_tag="ACHY09_004612"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFS3887481.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1531..2301)
                     /locus_tag="ACHY09_004613"
     CDS             complement(1531..2301)
                     /locus_tag="ACHY09_004613"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS3887482.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARHLKNIATRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2378..3178)
                     /locus_tag="ACHY09_004614"
     CDS             complement(2378..3178)
                     /locus_tag="ACHY09_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS3887483.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3419..4333
                     /gene="yafC"
                     /locus_tag="ACHY09_004615"
     CDS             3419..4333
                     /gene="yafC"
                     /locus_tag="ACHY09_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFS3887484.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4330..5133)
                     /gene="dkgB"
                     /locus_tag="ACHY09_004616"
     CDS             complement(4330..5133)
                     /gene="dkgB"
                     /locus_tag="ACHY09_004616"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFS3887485.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVVWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5295..5371)
                     /locus_tag="ACHY09_004617"
     tRNA            complement(5295..5371)
                     /locus_tag="ACHY09_004617"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5335..5337),aa:Asp,seq:gtc)
BASE COUNT         1415 a         1321 c         1345 g         1331 t
ORIGIN      
        1 caacaacctg aagaacgttt tgcatggtta aggtcaaaga aagataggtt ctgataaaac
       61 tttcttgtca tcggctccgt tcgccgttat gatcggtcgt cttttaagca actattgaca
      121 cacacatgaa ggcaaaagcg atattactcg cctctgtcct gctcgtgggt tgccagagta
      181 ccggcaacgt tcaacagcac gcacagagcc tttctgcagc tggtcaaggg gaagcagcaa
      241 agtttacaag tcaggcacga tggatggacg atgggacgtc tatcgcgcca gatggtgact
      301 tgtgggcttt cattggcgac gagctaaaga tgggaattcc ggaaaatgac cggattcgcg
      361 aacagaaaca gaaatattta cgcaataaga gctatctcca cgatgtaact ttacgggcag
      421 agccgtatat gtactggata gccgggcaag ttaaaaaacg taacatgcct atggaactgg
      481 tactactacc catagtggag agcgcttttg atcctcacgc aacgtctggc gccaatgccg
      541 caggcatctg gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa cagacccgca
      601 attatgacgc gcgtcgcgat gttgttgctt caacaactgc cgcgctgaac atgatgcagc
      661 gtctgaacaa aatgtttgac ggcgactggc ttctgaccgt agctgcttat aacagcggcg
      721 aaggtcgagt catgaaggca attaaaacga acaaagcgcg tgggaaatcc acggacttct
      781 ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taagatgctg gcattgagtg
      841 atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat gaaagccgtg
      901 ctctggcacg tgtgcacctg agcagcccgg ttgaaatggc gaaggttgca gatatggcgg
      961 ggatttccgt cagcaagctg aagacattca acgcaggcgt gaaaggctcc acgctgggcg
     1021 caagtggtcc gcagtacgtg atggtgccaa agaagcatgc agatcaactg cgtgaatctc
     1081 tggcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat acgccgctta
     1141 acagccgtgt ttacaccgta cgctctggcg acacgctttc aagtatcgct tcacgtctcg
     1201 gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaag ctgaagccag
     1261 gccaaagttt gacgattggt gcaggcagta gcgcacagcg actggcaaac aacagcgata
     1321 gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa cgccacggcg
     1381 tgaacatcaa agatgtaatg cgctggaaca gcgacactgc gaatctgcaa ccaggcgata
     1441 agctgacgtt gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc agataataaa
     1501 aggcaccgat tcccccggtg ccttttttat ttatgccgct ttatgtgctt ctaccatgat
     1561 gatatcactg gtgaaagagc catcattctg caaggcaaaa tacgttttca cctctgtcga
     1621 tgcgctctgt tggtaaatgc gaatagcgtc tactaacgct tctggcgtac gcattctcgc
     1681 gacccatgaa gaaaattcca gcggtaactt atctgtaatt aaattatcaa ctatcagatt
     1741 ggcttcattg attaacgtca accactcacc gctggcgtag tttcgtacgt gagaggtatc
     1801 gcgtaatgct tctaccgtct gtaaccagac gtcgcgcact gggtgacccg gagacattac
     1861 gtccatcaca atcagcctac cgccaggttt caatatccta ttcacttctc gcagtgctgc
     1921 accaacatca tgccagtgat gggcagaata acggctgata acaatatcaa atgcgttatc
     1981 ggcaaatggc agactttcgg catatccctg gcgggtggcg atatttttca ggtgccgggc
     2041 ttcggcagct tgtgccacga catccagcat ttgggcagat aagtcatacg ccaccaccgc
     2101 gctcacgttc tgcgcagcga caaagctggc atgccctgct ccgcagccca tatcaagtac
     2161 acttgcatca ggataatcag ccagacgcac cgccaggcgc tgtaaatctc gcccggatgc
     2221 atgtacggta ctggttaaat actcactggc ctgcgagcta aactgctttt ctacgtggtc
     2281 atggtgggat tgtgttgtca ttgcgctgtc cttttattgt ttgattaaaa agggcagcgc
     2341 caaacgttgg tctgccctgt ggcagacctg acatacctta tttatcaggc ttgccgggac
     2401 tgaattcaac gagtagcgga ttgtgatcgg aagcgcgcgt aaccagtaca gaagcttcgc
     2461 tgacgttcag accacggtag aaaacaaaat cgagcgggcg gccaaacgcc cgacggcgct
     2521 gatcatcggt aaaacgcacc tggcgcagcg acatttcccg cgcaaagcga tataacgcgt
     2581 tcatccttct acggctccag gcattgaaat ctcccgccat aatgaccggg ccgctgtggt
     2641 gagctatctg atcgccaata ggaagtaact gcttactata gacatccacg cccaaactga
     2701 aattgacggc gtgtatatta accaccatca acaggcgggt gtcaggcaat ggatagaccg
     2761 tcaccagtgc cgacttcgcc agacgcaaaa tgggttctcg ttcacgtaac gggcagcaat
     2821 acactggatg cgccgccgaa agggtcatta caccggaagg atgttgtggc agcacgaaag
     2881 cgggtacctg atcggcggca agatagttag cggtcgcaaa ctgcactaac tctggcgttg
     2941 tctgcgcttc ctgcaataac accagatgtg catctttgcc gtagttcttt aataccgaca
     3001 accattcagc gcgttgctgt ttgtatatgt tccacaccag gatccgaata cgctcttcgg
     3061 tacttaacgg ttctccaggt ggtaatgcct ggccgatgct cgcaaaagac cccggcggta
     3121 agatcctttc cgcaggttgt ccagcaacat agcgcatggc ataggtgttt tttcgcactt
     3181 ttgactttga aacctctatt acgtcaaccc ttccgcactc gggagggttc tactgttata
     3241 gggattttag ctcactcttt caacgagcaa ttgtatattg ctatgtaagc aagtgcttac
     3301 accggtcaga aatttgctca ttattgagaa cattaaatcg gatgacatat ctcgcatcct
     3361 gatggtttaa tgacaattaa attcaaaata tctcttctat tttgcaacag gagcaaaaat
     3421 gaaagccacg tcggaagaac tcgccatttt tgtttcagtc gtcgaaagcg gcagctttag
     3481 ccgggcagcg gaacaattag ggcaagcaaa ctcagcggta agccgggcgg tgaaaaagct
     3541 ggagatgaaa cttggcgtta gcctgcttaa tcggaccacg cggcaactta gcctgacgga
     3601 agaaggcgag cgttattttc gtcgcgtaca gtcaattttg caggagatgg cagcggcaga
     3661 atcagaaatt atggagacgc gtaatacacc gcgtggactg ttacggatcg atgccgcaac
     3721 tccagtggtg ctgcactttc tgatgccgtt gattaagcct tttcgtgaac gctatccgga
     3781 agtcacttta tcgctagtct cctccgaaac gattattaat ttgatcgaaa gaaaagtgga
     3841 tgtcgcgata cgcgctggta cgttaacgga ttccagctta cgtgccaggc cgttatttaa
     3901 cagttatcga aaaattatcg cctcccccga ttatatttcc cgctacggga agccagaaac
     3961 gatcgacgat ttaaagcaac atgtttgcct gggattcact gaacccgctt ccctcaatac
     4021 ctggccgata gcctgtagtg atggacaatt acatgaggtg aagtacggtt tgtcatccaa
     4081 tagtggggaa acactgaaac agctttgcct gagtggcaac gggattgcgt gtttgtccga
     4141 ctacatgatc gacagagaaa tcgctcgcgg agaattggtg gagttaatgg cagataaagt
     4201 attgccagtg gaaatgccat tcagtgccgt atattacagc gaccgtgcgg taagtacgcg
     4261 catccgggct tttatcgatt tccttagcga gcatgtaaaa acagctcccg gaggagctgt
     4321 cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc ggtcgttgca
     4381 atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag
     4441 attactttcc aggtttttac gtttagtaga agaaggaatt acggagtaac cttcccccat
     4501 agcccacgcc agaatcactt gtgccggagt cgcattgtgt ttagctgcga tacgagcaat
     4561 aacctcatct ttcagggcct taccatacgc cagcgtcata taggaagtaa tatggatgcc
     4621 gtgctgttta gcccagacga ccactttacg gttttgcaga taaggagaga gttcaatctg
     4681 gttagtagcg atgttttcag cgccaacagc agcaatcgcc ttttccatca atgggatcgt
     4741 gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca gcagcgcctg
     4801 cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc
     4861 aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca atttgtcttt
     4921 gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg gcacgccact
     4981 ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg tatcaattgc
     5041 gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt ctttcagacg
     5101 gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
     5161 ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa acgcagagga
     5221 tttttgcgat tctggcaata atagaaatac agacgaaaaa aacctcgagc gttaactcga
     5281 ggcttcttaa taagtggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg
     5341 caggtattct aaccgactga actaccgctc caccgaattc ttttacaacc accggtttta
     5401 tgaccggctt ac
//
LOCUS       DAZUCC010000271         5379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZUCC010000271 DAZUCC010000000
VERSION     DAZUCC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.273"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(112..2682)
                     /locus_tag="ACHY09_004618"
     CDS             complement(112..2682)
                     /locus_tag="ACHY09_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS3887486.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADKAVSTDSVTLNKDKTT
                     LDQDVVINGDNKITAVTIETSDSDKDLNVTFGGHDITAASTVNQGFVEGVKVSGDKNV
                     VINATGSTITAQGEGTYVRSAMVISSTGDVVVNGGNFVAKNEKGSATGISLEGAQGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSVLNGFNGDATDNITLAGSNIINGRIETIL
                     IAQENKGTHTVNLKIKDGSVIGAANNKQTIYASASAQGKGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYAYAYAGVYADDDKASSNLNINLSDGSV
                     WKGNVAGSTTTGSTGTTENQSATVSLSDSTWKGNVGDGLDGGARVTLKNSSWTGDVGN
                     AKTAVFLTDGSVWNGAVSINAPVDRASVPMGSVQPNKSSVSLQNGSVWNVTGASTVDA
                     LAVKDSTVNITKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAGSLDLI
                     NEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSVIQAMA
                     AAPANVANLQADTLSARQDAVRLSENDEGGVWIQYFGGKQKHTTAGNASYDLDVNGVM
                     LGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGIFIDTA
                     AQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAKLSALT
                     LEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFSDGNKV
                     RLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVGINVTW
                     "
     gene            complement(2724..3485)
                     /gene="hyxA"
                     /locus_tag="ACHY09_004619"
     CDS             complement(2724..3485)
                     /gene="hyxA"
                     /locus_tag="ACHY09_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFS3887487.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVANAGTQALSLHKYK"
     gene            complement(3639..4433)
                     /gene="hyxR"
                     /locus_tag="ACHY09_004620"
     CDS             complement(3639..4433)
                     /gene="hyxR"
                     /locus_tag="ACHY09_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFS3887488.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGKLVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            complement(4763..5326)
                     /gene="ipbA"
                     /locus_tag="ACHY09_004621"
                     /gene_synonym="fimX"
     CDS             complement(4763..5326)
                     /gene="ipbA"
                     /locus_tag="ACHY09_004621"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFS3887489.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKCRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT         1409 a         1309 c         1156 g         1505 t
ORIGIN      
        1 aatatcagca aatatccagc atcatgaata ctggatattt atagcctgca ggcagcatac
       61 tgctgcctgc agggggtata acagtcgaca ggggaaccga ctgctttatt cttaccaggt
      121 cacattgata ccaactacac cctgtagcgg attctcaatg tcgtcacctt tggtgtagtc
      181 aaggcttgca tatgctccca tgtttttggt gatatcagcc tgtacacctg cacccacgcg
      241 gaatgctgca ccgtcaatgc tggcattgac agactcatca cccagacgga ctttgttgcc
      301 atcagagaat tcgttcagtg cggccagatt cagataaggt ttaacggtcg agttgcccac
      361 agcgaagtca taacccacgc gcgtaccggc ctcacccagc acagagttat agctgtcaga
      421 atgaacgtcc acgccgttga gttgataatc cacaccttcc agcgtcagag cagacagttt
      481 ggcatatggc tgaataaaca ggccattacc gtctttccat gtgtaaccgc ctttaaccat
      541 cgcaccaaaa ccattggtgt taaagtcagc tttgatggta ccgccaccat tcatcaggcg
      601 aacatctgcc gtgttgctgt aatgaccaaa ctgtgcagca gtatcaatga agataccgtt
      661 gttatactgg cggctcaggt aagcgtggaa gctgtaacct tcagtgtcac ctttgctctg
      721 catggtggtc atgtcacctt tcgcagaaga catcgccaca ccggccagcc agctaccatc
      781 ttcagtcatg aagcgggtat caccacccag cattacaccg tttacatcca ggtcatagga
      841 tgcatttcct gcggtggtat gtttctgttt accgccaaag tactgaatcc atacgccacc
      901 ctcgtcattt tcgctcagac ggacagcatc ctgacgggcg gagagcgtgt cggcctgcag
      961 attagcgacg ttagccggag cagctgccat tgcctgaatc acggagctgg cgttggatgc
     1021 tttatgcgtg ttttttacga aataaaagtt accatcagct ccctgtgtca ggtcatattg
     1081 atacagacca ccttccgttg tgcctgtggc ttttagagtg ctgtctttgc cggtagaaat
     1141 aaatgccgtt tgttcattga taagatccaa tgaacccgca ctgtaaacac gcaagtcacc
     1201 gctcgcttta ccgctgatat ccagggtgtt ttcagaagag gcatcaacaa tcagagtgcc
     1261 gttttgagaa gcaaacgtgc cagtattgac tgtagccttc gtgatattaa ccgtactgtc
     1321 ttttactgcc agagcatcaa cagttgaggc tcccgtaaca ttccagacag acccgttttg
     1381 cagagataca ctgcttttat ttggttgaac cgagcccatt ggtactgatg cgcggtccac
     1441 tggcgcgtta atggaaacag caccattcca cacagaacca tccgtcagaa aaacagcagt
     1501 tttggcgttg ccgacatcac cagtccagct ggagtttttc agcgtgactc gcgcaccacc
     1561 atccagaccg tcacccacgt tacctttcca ggtggaatca gacagactta cggttgctga
     1621 ctggttttct gtagtaccgg tgctgccagt agtcgtacta cccgcaacat ttcctttcca
     1681 gactgagcca tcggaaagat ttatattcag attactactc gctttatcgt catcagcata
     1741 aacaccagca taagcataag cataagcatt accttcccag taagagcggg caacgttcat
     1801 atttacattt gttgtggtag cggctgaatt ctcgctttca gaaagggcca ggacatcaga
     1861 gtagatggtt gaatcagcaa cagacaaatt taaattttgc gttgctgaac cttttccttg
     1921 tgccgaagct gaggcataaa ttgtttgttt attattagcc gccccaatta ctgagccatc
     1981 cttaatcttc aggttgactg tatgagttcc cttattctcc tgagcaataa gtattgtttc
     2041 aatccgacca ttaataatat ttgagccagc aagggtaatg ttgtctgttg catcaccgtt
     2101 aaaaccattc aatacgctac ccttttgagc aaaaattgcc gtggaggcgt tgctggaact
     2161 cttattacct tgagcattta tggttgtgcc attaagcgtg acattatttc cctgggcccc
     2221 ttccagagat atccctgtcg cactaccttt ttcatttttt gcaacgaaat taccgccatt
     2281 aacaacaaca tcgccagttg aagaaatgac cattgcagac cggacatagg tgccttcacc
     2341 ttgagctgtg atggtggagc ctgtagcatt aatcacaaca tttttgtcac cgctaacttt
     2401 tacaccttca acgaaacctt ggtttaccgt tgatgcggcg gtaatatcgt gaccgccaaa
     2461 agtaacatta aggtctttat ctgaatctga cgtttcaatt gttacggctg taatcttgtt
     2521 atcaccgtta ataacaacat cttgatccag agttgtctta tctttattca gcgtaaccga
     2581 atcagttgat accgctttat cagcagcgaa tgctggtgca gaaagagcca taacaatagc
     2641 tgttgctaat gcgctgcggt aaaatgcctt actgtttttc atgaagaaat tctccattct
     2701 ggtaactaat ttcaatttcc cacttattta tatttatgca gagataaagc ctgcgtccct
     2761 gcatttgcca ccacggaata cctgttggta tttccgtttt cacgcaatgt catggcattc
     2821 gttgtttcca atgcatttcc atcaccgtga aaattattgc tgctcagcag cagggcttcc
     2881 cgcaatgaca tgctgtccgg tattccccaa cgctttttta gcgactgctc ggcaagccat
     2941 atctgtctta tccccgtttt gagaaactgc gcactgtatt cccagctgtg tccggcaaga
     3001 agcaacgaca acaccttctt ttgcttttcc gggaagcgtt ctgacatgac gattgtcttt
     3061 gtgcggttat tcacctcttt cataaagaca gagtaactgt ccataactgg caaaatttct
     3121 gcatgtttca gccctgaaaa cccaccagag gcatttgcaa tactgatgac acgctctact
     3181 gacattcctg gtttcagtaa cctcaggttt ttgagaacgc cagaaatact cacatctggt
     3241 ggcagaaaaa agggtgcgac gcacaacacc accacgaatg agtgatcccg ataaaatcgg
     3301 aaccacattt cagcgcattc ggcaggtcgc cctgccgata tcacgacact ctgtggcctg
     3361 tgccgcaatt tattgcgtaa ctcctcttct gttctgatgc aggtaacatt gccgacagaa
     3421 ccggttgata gagctgcgaa ctgcgctggt acaattcctg agggacagaa aagaacgaaa
     3481 cgcatccttc cctcacagat gtcagtacgc cagcacgctg tacaggctgt acagcgtgcc
     3541 tcccaatcct gcagcccaga gcagtagaaa tattttggct cctacactgt agcgagtccg
     3601 tatgcgccgc agtggggggt agtaacaacg ctgctgtgtc ataacaatcc tggtcttttt
     3661 ttagacatat tttttctttg tccggaaagt acttttccgg atatggcgtc agaatttatt
     3721 gattcaccaa cggcaacttt ctctggataa agaattccgg ataccatgtt ctgcacatac
     3781 tgagtgagtc gatgagcgga atgttgtccg atatattttg catgtagccg tatattccgt
     3841 ttcactctgc tgtcaccgca tcccgtatag agcagcctga aatcgctcag tgttcccagt
     3901 ccaagtttca ttaaagcccg tgtggcaatc gcatgcaggg tctttgaact gcgttgttca
     3961 taacgggcaa tctcactgaa actcataccc tgcatcagaa tcttaagcac gctcaactcc
     4021 cgtgcagtca ggagccagaa atcgctcata tcgccgggca aaggacgtag taaccagcgg
     4081 ataatttgat tctgataacg actcgctgtc agcatggtgc cgatgcgtgg gaagccgtta
     4141 tacagacttg cagcaggagc attatcgaac cagtcggagt caccggtcag gcagacctgc
     4201 cattgcccgg catcactccg caagcggaca agacaagcca gaagaaccaa ctttcccgaa
     4261 ggaagcgatg ggatatcagc taacaggtag ccacaggagg tctttaaaac gaattcaacc
     4321 tctgtagtga cgttgctgta cacccgcacg atatacctgt ccgttgggat gagagactcc
     4381 agtgccagcc tggtgaactg acaggcactg ataataacca gaggcttctt cattcagact
     4441 cgtccacgcc attgctacaa aacctgacat tttgattcaa aatgtcaggt ttggtttaat
     4501 tgcctgaata tttaatttta ttggaatata aatctaattt gtttgatttt tattgttttt
     4561 aattttgtgt tatttaacaa cgcgagtgaa aactgatcac tggtatgacc tttgtggtta
     4621 atcatttcaa atcatggggt tattttactt tagcgagaaa tatcaacatg ttatgtgttt
     4681 tatttaattg aatgagctgt ttttttatgg aagaattgag gcaaacgtag gaatctgaca
     4741 ttttgaatca gaaggtactg ttttaacgag gctttttttt ccacacccct ttaaatcgag
     4801 cagcattact ggctgtgtat ctgacagtat gctgaatatt tcgatgaccc agataatcct
     4861 gaagtagtcg ggtatcgaca ccattgtcag ccagagcaaa tccacaggca tgacgtaaca
     4921 tatgaggatg tgcttttaca gctaatcctg cacgtcttcc tgcctcacga atgatcgaaa
     4981 aaaaatgttg tctgctgata gggcggcatt tccgggagag aaaaagccag tctccttcaa
     5041 ctccattttc aattaacttc ctctgcttaa gccacaactt gatcaaatta tattcatcag
     5101 gaagaagagg atgtgttgtc gagaagccat ttttaatacg ccggatatta agttgttttc
     5161 ctgatgcatc aatatcggat aaacgtaaat caagaagttc actggcccta aaaccgtgaa
     5221 tgaaagccat cataattaga cagcgatttc tttcaggaaa ggacatgctc tgggctgcat
     5281 ccatcagcct gtagacttca tcctgtgtga gatattttct cgtcatacgc caccgtgtac
     5341 agttgtaaaa aaaagaggca cacccagata agtgtgcct
//
LOCUS       DAZUCC010000272         5357 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZUCC010000272 DAZUCC010000000
VERSION     DAZUCC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.274"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            205..1539
                     /gene="cadB"
                     /locus_tag="ACHY09_004622"
     CDS             205..1539
                     /gene="cadB"
                     /locus_tag="ACHY09_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFS3887490.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTAGTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAGSGAPFAISASTILGNWAAPMVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1619..3766
                     /gene="cadA"
                     /locus_tag="ACHY09_004623"
     CDS             1619..3766
                     /gene="cadA"
                     /locus_tag="ACHY09_004623"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFS3887491.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSSWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYEDMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3825..5282
                     /gene="dtpC"
                     /locus_tag="ACHY09_004624"
     CDS             3825..5282
                     /gene="dtpC"
                     /locus_tag="ACHY09_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFS3887492.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT         1258 a         1282 c         1305 g         1512 t
ORIGIN      
        1 cttttcatga tatcaacttg cgatcctgat gtgttaataa aaaacctcaa gttctcactt
       61 acagaaactt ttgtgttatt tcacctaatc tttaggatta atcctttttt cgtgagtaat
      121 cttagcgcca gtttggtctg gtcaggaaat agttatacat catgacccgg actccaaatt
      181 caaaaatgaa attaggagaa gagcatgagt tctgccaaga agatcgggct atttgcctgt
      241 accggtgttg ttgccggtaa tatgatgggg agcggtattg cattattacc tgcgaaccta
      301 gcaagtatcg gtggtattgc tatctggggt tggattatct ctattattgg tgcaatgtcg
      361 ctggcatatg tatatgcccg actggcaaca aaaaacccgc aacaaggtgg cccaattgcg
      421 tatgccggag aaatttctcc tgcatttggt tttcagacag gtgttcttta ttaccatgct
      481 aactggattg gtaacctggc aattggtatt accgctgtat cttatctttc caccttcttc
      541 ccagtattaa atgatcctgt tccggcgggt atcgcctgta ttgctatcgt ctgggtattt
      601 acctttgtaa atatgctcgg cggtacctgg gtaagccgtt taaccactat tggcctggta
      661 ctggttctta ttcctgtggt tatgactgct attgttggct ggcattggtt tgatgctgca
      721 acttatgcag ctaactggaa taccgcaggt actactgatg gtcatgcaat cattaaaagt
      781 attctgctct gcctgtgggc cttcgtgggt gtggaatccg cagctgtaag tactggtatg
      841 gttaaaaacc cgaaacgtac tgttccactg gcaaccatgc tggggactgg tttagcaggt
      901 attgtttaca ttgctgcgac tcaggttctt tccggtatgt atccgtcttc tgtaatggca
      961 ggctccggtg ctccgtttgc aatcagtgct tctactatcc tgggtaactg ggctgcgccg
     1021 atggtttctg cattcaccgc ttttgcgtgc ctgacttctc tgggctcctg gatgatgctg
     1081 gtcggccagg caggtgtgcg tgccgctaac gacggtaact tcccgaaagt ttatggtgaa
     1141 gtcgacagca acggtattcc gaaaaaaggt ctgctgctgg ctgcagtgaa aatgactgcc
     1201 ctgatgatcc tcatcactct gatgaactct gccggtggta aagcatctga cctgttcggt
     1261 gaactgaccg gtatcgcagt actgctgact atgctgccgt acttctactc ttgcgttgac
     1321 ctgattcgtt ttgaaggcgt taacatccgc aactttgtca gcctgatctg ctctgtactg
     1381 ggttgcgtgt tctgcttcat cgctctgatg ggcgcaagct ccttcgagct ggctggtacc
     1441 ttcatcgtca gcctgattat cctgatgttc tacgctcgca aaatgcacga gcgccagagc
     1501 cactcaatgg ataaccacac agcatctaac gcacattaat taaaagtatt ttccgaggct
     1561 cctcctttca ttttgtccca tgtgttggga ggggcctttt ttacctggag atatgactat
     1621 gaacgttatt gcaatattga atcacatggg tgtttatttt aaagaagaac ccatccgtga
     1681 acttcatcgc gcgcttgaac gtctgaactt ccagattgtt tacccgaatg accgtgacga
     1741 cttattaaaa ctgatcgaaa acaatgcgcg tctgtgcggt gttatttttg actgggataa
     1801 atataatctc gagctgtgcg aagaaattag caaaatgaac gagaacctgc cgttgtacgc
     1861 gttcgctaat acgtattcca cactcgatgt aagcctgaac gatctgcgtt tacagattag
     1921 cttctttgaa tatgcgctgg gtgctgctga tgatattgct aacaagatca agcagaccac
     1981 tgacgaatat atcaacacta ttctgcctcc gctgaccaaa gcactgttta aatatgttcg
     2041 tgaaggtaaa tatactttct gtactcctgg tcacatgggc ggtactgcat tccagaaaag
     2101 cccggtaggt agcctgttct atgatttctt tggtccgaac accatgaaat ctgatatttc
     2161 catttcagta tctgaattgg gttctctgct ggatcacagc ggtccacaca aagaagcgga
     2221 acagtatatc gctcgcgtct ttaacgcaga ccgcagctac atggtgacca acggtacttc
     2281 cactgcgaac aaaattgtcg gtatgtactc tgctccggca ggcagcacca ttctgattga
     2341 ccgtaactgc cacaaatcgc taacccacct aatgatgatg agcgatgtta ccccaatcta
     2401 tttccgcccg acccgtaacg cttacggtat tcttggtggt atcccacaga gtgaattcca
     2461 gcacgcaacc attgctaagc gcgttaaaga aacaccaaac gcaacctggc cggtacatgc
     2521 cgtaattacc aactctacct atgatggtct gctgtacaac accgacttca tcaagaaaac
     2581 gctggatgtg aaatccatcc actttgactc cgcgtgggtg ccttacacca acttctcgcc
     2641 gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta gaagggaaag tgatttacga
     2701 aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga tccacgttaa
     2761 aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca ccacctctcc
     2821 gcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag gtaatgctgg
     2881 taagcgtctg atcaacggtt ccattgaacg tgcgatcaag ttccgtaaag agatcaaacg
     2941 tctgagaacg gaatctgatg gctggttctt tgatgtatgg cagccggatc atatcgatac
     3001 cactgaatgc tggccgctgc gttctgacag ctcctggcac ggcttcaaga acatcgacaa
     3061 tgagcacatg tatcttgacc cgatcaaagt caccctgctg accccgggga tggaaaaaga
     3121 cggcaccatg agcgactttg gtattccggc cagcatcgtg gcgaaatacc tcgacgaaca
     3181 tggcatcgtt gttgagaaaa ccggcccgta taacctgctg ttcctgttca gcatcggtat
     3241 tgataagacc aaagcattga gcctgctgcg tgctctgact gacttcaaac gtgcgttcga
     3301 cctgaacctg cgtgtgaaaa acatgctgcc gtctctatat cgtgaagatc ctgaattcta
     3361 tgaagatatg cgtattcagg aactggctca gaatatccac aaactgattg ttcaccacaa
     3421 tctgccggat ctgatgtatc gcgcatttga agttctgccg acaatggtta tgactccgta
     3481 tgcggcgttc cagaaagagc tgcacggtat gaccgaagaa gtttacctcg acgaaatggt
     3541 aggtcgtatt aacgccaata tgatccttcc gtatccgccg ggagttcctc tggtaatgcc
     3601 gggtgaaatg atcaccgaag aaagccgtcc ggttctggag ttcctgcaga tgctgtgtga
     3661 aatcggcgct cactatccgg gctttgaaac cgatattcac ggtgcatacc gtcaggctga
     3721 tggccgctat accgttaagg tattgaaaga agaaagcaaa aaataattag ctcgtacaag
     3781 ggaagtggct tgccacttcc cttttttgct cataaggaga acacatgaaa acaccctcac
     3841 agccgcgcgc gatatactat atcgtggcga tccaaatctg ggagtacttc agtttttacg
     3901 gcatgcgtgc cttactcatt ctctatctca cccatcagct tggttttgat gataaccatg
     3961 ccatcagcct gttcagcgca tatgcttctc tggtttacgt tacccctatt ctcggcggct
     4021 ggcttgccga ccgcctgctc ggcaaccgca ctgcagtgat tgccggcgcg ctgttaatga
     4081 cccttggcca tgtggtgctg ggcattgata caaattcaac ctttagcctg tatctggcgc
     4141 tggcaatcat tatttgtggc tacggtttat tcaaatcaaa catcagctgt ttgcttggcg
     4201 agctctacga cgagaacgat catcgacgtg atggcggttt ttcgctgcta tatgctgcgg
     4261 gcaatatcgg ttctatcgcc gcgcccatcg cctgcggcct ggctgctcag tggtatggat
     4321 ggcatgttgg ctttgccctt gcgggaggcg gcatgtttat cggtttgttg attttcttaa
     4381 gtggtcatcg tcatttccag tccacgcgta gtatggataa aaaagcgctc actagcgtca
     4441 aatttgcctt accagtatgg agctggttag tggtgatgct ctgtttagcc ccagtatttt
     4501 ttaccctgct gctggagaac gactggtccg gatatttgct ggcgatcgtt tgcctcattg
     4561 cagcacaaat cattgcccgc atgatgatca aattccccga acaccgccgt gctctttggc
     4621 aaattgtatt gttgatgttt gtcgggacgt tgttctgggt actggcacaa cagggcggca
     4681 gtaccatcag cttgtttatc gatcgctttg tgaatcgtca ggcattcaat attgaagtac
     4741 ctacagcact attccagtcg gtgaatgcta ttgcggtgat gctcgctggg gttgttctgg
     4801 cctggctggc gtcgccagaa agccgcggca attcagcatt gcgcgtctgg ctgaagtttg
     4861 cctttggcct actgctgatg gcttgtggct ttatgttgct ggcatttgat gcccgacatg
     4921 cagcggctgg cggtcaagcg tcaatgggcg tgatgatatc cgggctggcg ctaatgggct
     4981 ttgctgaact ctttattgac ccggtggcga ttgcgcaaat cacgcgtctg aaaatgtctg
     5041 gcgtattaac cggtatttat atgctggcaa caggcgcggt cgccaactgg ctggcaggcg
     5101 tcgtggcaca gcagacgaca gagtcgcaaa ttagcggtat ggcaattgcc gcttaccagc
     5161 gattcttttc tcagatggga gagtggacgt tggcttgtgt cgcgatcatc gtggtattgg
     5221 cctttgctac ccgttttctg tttagcacgc cgacgaatat gatacaggag agcaacgatt
     5281 aactgtcgta aaaacaattg gttatgaata aattaacttt cagtttatca cactagcaaa
     5341 tcgagattaa taagtca
//
LOCUS       DAZUCC010000273         5207 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZUCC010000273 DAZUCC010000000
VERSION     DAZUCC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.275"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(426..3539)
                     /gene="mdtF"
                     /locus_tag="ACHY09_004625"
     CDS             complement(426..3539)
                     /gene="mdtF"
                     /locus_tag="ACHY09_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFS3887493.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGDHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(3564..4673)
                     /gene="mdtE"
                     /locus_tag="ACHY09_004626"
     CDS             complement(3564..4673)
                     /gene="mdtE"
                     /locus_tag="ACHY09_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFS3887494.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            4781..5059
                     /locus_tag="ACHY09_004627"
     CDS             4781..5059
                     /locus_tag="ACHY09_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887495.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYIYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5060..>5207)
                     /gene="gadE"
                     /locus_tag="ACHY09_004628"
     CDS             complement(5060..>5207)
                     /gene="gadE"
                     /locus_tag="ACHY09_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFS3887496.1"
                     /translation="LKNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGI
                     TSYF"
BASE COUNT         1235 a         1338 c         1353 g         1281 t
ORIGIN      
        1 agacaatatt atggtgtaac gccacatcag ttttcgcaac attcgccggg taccttttcc
       61 tgacatattt cgcatttgaa tattggtcag gatctcacgt ctgcttcatg tgaaactctt
      121 tcctgatgat ttctgccggg ctaccggcta gttctctttc gcagtttgcc aggcggtata
      181 ggtattttca ggaggaaaag actcggcatg tttgggatta ttaagctaac aattcatacg
      241 attacgggaa tgtgggtcag tattgtgctg ttcaaattga tgacgaatgg ctggagtggt
      301 ttctactttc agtgctgtgt actaagcctg gtattcctga cggttagctg gttgttgagt
      361 ggtgaatggt tagcaggaaa gagtaaggct gaaccctcac gttcaacctt actctcattt
      421 acacgttacg cttttttaaa gcgggcaaag agatgttcca ctacaacgaa aaagaccgga
      481 acgaagtaaa ttgccagcac tgttgcggca aacatcccgc ccattacgcc ggtacctacc
      541 gcgttttgcg cgccagagcc ggcaccatga ctgataacca gcggcagcac gccgagaata
      601 aaggccagag aagtcatcag gattgggcgt aaacgcatac gcgctgcttc gatgattgcc
      661 tctaccggcg ttttcccttc tttctgcatc atctcaacgg caaattcgac aatcaggatg
      721 gcgttcttgg cggaaagccc gatggtggtc agcaaaccaa cctggaagta aacgtcatta
      781 cttaagccgc gcagatcggt ggccagtaat gcgccaacga cgcctaacgg aacaaccaac
      841 atcaccgaga acggaattga ccagctttca taaagtgcgg cgagggcgag gaacaccacg
      901 accagtgaaa tggcatatag cgcgggagcc tggtttgagg ataacgcttc ctgatacgac
      961 agcccggtcc atgagtagcc gacgcctgcc ggaagttttg cgaccaggtc tgccataaat
     1021 ttcatggcgt caccggtact tttcccggca gccgcttcac ctaaaatctc cattgacggg
     1081 atgccgttgt agcgttccag tcgcggtgaa ccataggtcc attcggtaga cgaataggca
     1141 gaaagcggtg ccatcgtgcc agaggcgttg cgtacatacc attggttgat gttatccggc
     1201 aacatacgga acggcgtgcc tgcctggaca tacacttttt tcacccgccc ctggttgagg
     1261 aagtcgttca cgtagctgct gccgaaggcg gtggaaattg tctggttgat atcagacagc
     1321 gccacaccca tagcctcggc tttcgcagcg ttgacgttca ctttgaacat cggcgtatct
     1381 tccaggccgt tcgggcgtac cccgatgacc tgattcggtg attgcgctgc cagtgataac
     1441 agctcgtttc gcgcctgggt cagcttttcg tgccccagat tgccgttgtc cagcagttcc
     1501 atatcaaaac ctgacgcggt acccagttca gccaccgcgg gtaagttgaa cgggaagacg
     1561 acggctttat tgatactgct tagcgcaatc attgcccgct gaataatcgc ggtaaccgag
     1621 ttttcctcgc cgacacgttc agaccacggc ttgaggctga taaacgccag accgttgttt
     1681 tgcccctgac cactgaagcc aaagccacca acggtaaaca ccgactgaac attatttttc
     1741 tcttttgtca gataataatc cgtcacctgt tgtagcactt tcgtggtgtt aaccatggtt
     1801 gcaccggaag gtaactgcgc ggtggtcata aataccccct gatcctcttc tggtaagaaa
     1861 gaggtcggcg tacgcaggaa cagcaccgcc atcccggcgc aaatcagcag gtagaccacc
     1921 atgtagcgac cggtacaacg caacagcgag cgggtgctgt cggtatagtg ctgagttgat
     1981 ttttcaaaca gcgtgttgaa gcgtgcgaac agggcgttag gtttgtgatc gccctccggc
     2041 gcggctttca gaatggtggc gcacagggca ggggtcaggc tcattgctac aaatactgaa
     2101 agcagcatgg aggagatcag cgtgatggag aactggcggt agatctcccc ggtcgcaccg
     2161 ctcataaagg ccatcggcat aaacactgcg gaaagaacaa cggcaatccc gaccagcgca
     2221 cgttggatct gccccatcga tttatgcgtc gcttccttcg gcggtagctt atcttccgca
     2281 atgacacgct cgacgttctc caccaccacg atggcgtcat ccaccagtaa ccctatcgcc
     2341 agcaccatcc cgaacatagt taacgtgttg atggtgaaac cgaccgccga caagatcgca
     2401 aacgtcccga gaataaccac cggtacggca atcgtcggga tgattgtggc gcggaaattc
     2461 tgcaaaaaca gatacatgac caggaagacg aggatgatag cctcaaccag cgttttgaaa
     2521 acttcctgaa tagaaatttt gataaacggt gtggtgtcgt aaggataaac cgtcttcagg
     2581 cttgcaggga aataagccga taaacggttc agctcctctt tcaccgctcg cgaggtatcc
     2641 agggcgtttg ctccggtagc cagtttgatg gcgatcccgg cagcaggttt gccgttatag
     2701 cgcgccacgg tggaataatc ttccgcccca agttcgacgc gggcgacatc acgcagcagt
     2761 acttgcgaac catcttgctg aactttcaac aggattttgc caaattcttc cggcgtttgc
     2821 agacgcgtct gcacaatgat cgaggcgttc agttgctggt ctgccgcctg tggcatgcca
     2881 cccagttgac caccggaaat ctggttattt tgcaccttaa tctgggaaat aacatcggaa
     2941 ggtaccaggt tatatttatt gagtttttgc gggtccagcc agatacgcat ggcgtactcg
     3001 gaaccaaaga gctgtacgct accaacgccc gcggtacggc ttagcgggtc tttgatatta
     3061 gacgctacat agtccgcgat atcgtactgg ttgaggctgc cgttatcaga aataaatgcc
     3121 gctaccatca ggatattact gctcgactta tcgacgctaa tcccctgctg ctgcaccgct
     3181 tcaggtaatg aaggcatagc gagctgcagt ttattttgca cctgaacctg tgcgatatca
     3241 ggagatgtcc cagtctcgaa ggtcagagtg atagaggcat tacccgccgc atcactggtt
     3301 gaagacatgt acatcaggcc atcaagccca ttcatatttt gctcaatcac ctgagtgacc
     3361 gagtcttcta ccgtttgcgc atcggcaccc ggataggttg cgctgacggt aatggtcggt
     3421 ggcgcaatct gcggatactg cgcaaccggt aagttcatga tcgccagacc acctgcaagc
     3481 atcataataa tggcaagtac ccaggcaaaa accgggcgat caataaaata gttagccatg
     3541 aaagtcccct taagcctgca acgttattgt ttcgattcgg tgctggcgtt ttcctggctg
     3601 gaggaaattg ctcgtgcttt gatacccgga cgaatgcgtt gcaaaccgga aacgatcacc
     3661 cgatcgccag cctgcaagcc agaggtgaca acccactggt cgccgatggc tttgctggct
     3721 tcaatttcgc gtagctgcac aacatcgtct ttatccagaa tgagcgccgt tgctttaccc
     3781 tgggcgttgt gggtgacgcc ttcctgcggc accagtaata cattctggcg gctaccttca
     3841 tccactaatg ccgtgacgta catgccaggc agcaagtcac catttgggtt ggggaaaatc
     3901 gcccgtaacg tcacggagcc cgtggtttca tccaccgtcg ggtcggagaa tttcagcgtg
     3961 ccggtctggc tgtaacgttt accattttcc agattgagct gtactggcgt actgccctga
     4021 acctgtttga tttgcccact ggcgacctct tctttcatgc gcaagaaatc ctgcaccgac
     4081 tgcgtgagat cgacataaat cgggtccaga cgttgtacgg taaccagcga atctgcctga
     4141 ttagcggtaa cgagtgcgcc gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc
     4201 gaggtgacat ttgcgtattg caggttgata gtcgcctgtt caacagccgc tttggcgacg
     4261 gtgacattgg cttctgcttc attcaactgg gtgcgcgcgg tgtcgtaatc ctgacggcta
     4321 acgtagttgg tcttcagcaa cgatgcctgg cggttaaagg tgatgcgggc attgctggcg
     4381 gtagagagcg ctttcgccag cgagcctttg gcggagttca gctctgcctg taaaggcgca
     4441 ggatcaatct gatacagcga atcgccctgg ttcactttat cgccttcgat aaagttgcgt
     4501 ttaatgataa taccgcccac ctggggacgt atctcggcaa cttcataagg aacggttcta
     4561 ccaggcaatt cgctcaacac attgaccgaa ccgggggaga gtgtgacgac accgacctca
     4621 ggcgtcatgg cggcggcgtt ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg
     4681 cagaataaca acggtattaa cagctttctt cttctgttca ttttagtccc tgaaaattct
     4741 tgagaaataa cggttcttta aattgcgcgc gaggagagat atgagggatt tacagaccag
     4801 cggcatagtg gggttgtcag ccagtaaggt gggatacagg cacagtgatc gacatggtga
     4861 ggtcaacgac aaaaaaaatg ttgccgttct gccaacagtt cctgccagca ttcgggcaac
     4921 gaagggttct actggtggat acatatacca ggggaataaa gtcgttttcg cctctccttt
     4981 agtaatcacc gggggtaatg aaatcagcat ctgcatccct cgtcatgcca gccatcaatt
     5041 tcagttgctt atgtcctgac taaaaataag atgtgatacc cagggtgacg atgtcgctca
     5101 tacgtcttag tttcagtttg tacataatgt tgaatttctg aatctttaat gtttgttgcg
     5161 aaatattgag cgtcgacgtg atattgcttt catttttatt tttcaga
//
LOCUS       DAZUCC010000274         5199 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZUCC010000274 DAZUCC010000000
VERSION     DAZUCC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.276"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..963
                     /gene="mscS"
                     /locus_tag="ACHY09_004629"
     CDS             118..963
                     /gene="mscS"
                     /locus_tag="ACHY09_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFS3887497.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIGGIAARMISGVLNNLMLGRNI
                     DATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLA
                     AGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIVN
                     FSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMNF
                     VVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRS
                     VSSRQ"
     gene            1089..2309
                     /locus_tag="ACHY09_004630"
     CDS             1089..2309
                     /locus_tag="ACHY09_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFS3887498.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            2539..4284
                     /locus_tag="ACHY09_004631"
     CDS             2539..4284
                     /locus_tag="ACHY09_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS3887499.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
     gene            complement(4466..5095)
                     /locus_tag="ACHY09_004632"
     CDS             complement(4466..5095)
                     /locus_tag="ACHY09_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFS3887500.1"
                     /translation="MSIEQIINEINVYLNSLKKEDERIDAINKIKIELHRSSPFYAEP
                     VDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGF
                     HRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIV
                     RDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEENFSPAWTIK"
BASE COUNT         1296 a          990 c         1330 g         1583 t
ORIGIN      
        1 gctgtgtgta tgatgcggga taaaggcccg gatattcctg gaaataaaat gttccgggga
       61 atataaaaca gggaaaaacc ggatgtgatg gacgcttctg gcgaggcggt ttcatgaatt
      121 gtccgtaatc aggatatgct gcccggttac gccgcgaatc ttgtggcagc agtggttatt
      181 attatcgggg gtatcgctgc acggatgatt tctggtgtgc tgaataatct gatgctgggc
      241 aggaatattg atgccacagt ggcgctgttc ctttccgcgc tgatacgtca tgccattata
      301 gcctttacgc tgattgctgc cctgggacgt gtgggggttc agacagcttc cgtaattgca
      361 gtcctggggg ctgcaggtct tgctgttgga cttgccctgc aggggtcatt gtcaaacctt
      421 gcagctggta ttcttctcgt gatgttccgt cctttcaggg ctggtgaata tgctgacctg
      481 ggtggtgttg ccgggactgt gcagaatgta catatattct caaccacgat gcgtacactg
      541 gatggtaaaa ttgttgtcat tccgaacggg aagattattg ccggagagat tgttaatttc
      601 tccagggagc ctgaacgacg aaatgagttt atcatcagtg tttcatatga tgcagatatt
      661 gactgtgtga aacaagtcct gacagatatt gtgatgtcag aagagagggt tctgaagaac
      721 agggaaatca ctgttcgcct gaatgagcct ggggagtcat ccatgaattt tgtagtacgg
      781 gtatggagca ggcgtgatga ccttcagtct gtatactggg atgtactgga gagaattaag
      841 cgggagtttg attcagaagg gataagtttt ccatatccac aaatggatat ccatcttgtt
      901 cgctccggga agcaggcaga gtcggtaacc cataacagtc gctcagtcag ttccagacag
      961 taaaatgaaa agcgcatttt atttgtgaaa gatgaacagc ctgaatctgt caggctgttt
     1021 tcattgtgtt tttatggtca tttttggtat atgtactgta tataatgtca tctgtacaag
     1081 gatataaaat gccaaagaaa ataatgacag ggattgatgt ttttgacgct gccatggagc
     1141 gtattgaatg ggtgtttgat acgttttcat cagtttgtct ttctttttcc ggcggaaaag
     1201 actccactgt cctgtttcac ctgatggcga atattgcccg ccgaaaaaaa cgccgttttt
     1261 ctgtactgtt tgttgactgg gaggctcagt atcagtgtac cattgagcat gtcaccaggt
     1321 tgagggaaat gtacgatgat atcacagata cttttttctg ggtggcgctg cctctgacga
     1381 ccgttaatgg agtgtctcag gtacagcctg agtgggtatg ttgggaggac ggggctgaat
     1441 gggttcgcca gccaccagaa gatgccataa ccagagcgtc tttttttcct ttttatcaga
     1501 atgcgatgac atttgaagag tttattccgg catttaatca ttggtttgcc cgggaaaaag
     1561 ggatggttgc tgcgattctt actggtgttc gcgctgatga gtcactgaat cgttttatgg
     1621 gactggtatc cggacgtaag ctccgttatg cggaggataa accctggacc acggcatcgg
     1681 ctgagggatt ttactacacc atgtatcctt tgtacgactg gaaaacaaga gatgtatgga
     1741 tttaccatgc gagaacacgt gctgcctata actctcttta tgacctgatg tacagggctg
     1801 gagttcctct tcgcaatatg cgggtgtgtg agccttttgg accggagcaa cgtaaggggt
     1861 tgtggcttta tcatgtactg gagccggata catggggcag aatgtgtgct cgcgtatcag
     1921 gtgctgtagg gggagcgctc tatgcaaatg aaagtggtgc ttattttgct ttgaggaaac
     1981 agataacaaa accagttcac catacatggc agagttatgc aatgtttctg ctggagtcaa
     2041 tgcctgagaa aacggcggag cattacaaga ataagattgc tgtttatctt cggtggtggc
     2101 agtctcatgg atggccggat ggcatccctg atgagcagga taatgaccag ggatgtaaag
     2161 atattccctc ctggcgtcgc atctgtaaga cgttactgaa aaatgatttc tggtgtcgga
     2221 tgctggcgtt tagtccgaat aagccccgac attatgagcg ctacctggcc agaatgcgtg
     2281 aaaaaaggaa agagtgggga atattgtaaa ttatatttaa acatcagaaa tatgacggtt
     2341 aatccaggat agtagctaaa aaaataagat tccttgctga tttatccctg tttgatatgt
     2401 aaaatctgag gggcttataa gcaacggata tacgagtgct acaatatatg ataagttata
     2461 tatattaaat gttcatcatc tgtcaggggc tgttttatgg ctaaactgat aaatatatca
     2521 ggtgttaccg aggagcggat gactgccggg gaaaagcggg ttgcatcccg tcttgaggca
     2581 tttatgagta atgagtgtct ggtctggtac gatattccgg tgggacggaa aaaccgacat
     2641 cctgattttg tcattattga cccggagaat ggtctggtat ttcttgaagt caaggactgg
     2701 accgttcaca cgttgcatca ggtaaatcac gaacaggtca ttcttgaaac taacggtatt
     2761 cttaaatcag aaataaatcc gctgctacag gttcgtcgtt atgcctgtga taccgtaaat
     2821 gctttacctg cagactcacg tttgcgacag aatgatggtc agtataaggg gcgtctgaat
     2881 ctggcctggg cgtatggtgt tgtgtttacc cgtattaccc gccagcaact gaaggctctg
     2941 gctgggaatg atgaaaatgc tgtggaaagg attttccctt cagcgcagac aatctgtcag
     3001 gatgatatga ctcagtctgt tttacctgat atattccgcc aaaaaattgc cgggatgttt
     3061 acgatgggat tcagaacccg ggtgacacca caggttagag atattctgcg tgctcatctg
     3121 tttcctgaaa tgaccgtcag acagcactca caaatcaaaa taatggatat tcagcaggag
     3181 attctggcca gaaatattgg tgatgggcat cgtgttattc atggggtagc cggctctgga
     3241 aaaacaatga ttcttttgtt ccgttgtctt tacttggctg aaactacccc tggtaagatt
     3301 ctggtgctat gttacaacat tactctggcc agctacctgc gggaatgtat tgagtccagg
     3361 ggactgaagt cacgggttac ggtattccat tttcatagtt ggtgcgcctc aatgataaag
     3421 cgccatggaa ttcaggtgac tgctggagga aaggattacc cggagaaatg cttcagtgcg
     3481 ctggaagatg ccgtgaacag cggaacaatt actgatactg gatacgatgc cgtacttgtg
     3541 gatgaggggc atgattttga gagtcgctgg ctggctctga tagcccgcct gtttgataac
     3601 gcgtcccgtt cactgctcct gatgtacgat gacgcacagt ccatttacag acgggaacgg
     3661 gctctgaact tttcgcttgc cagtgtgggg attcaggcgc agggacgtac ttccgtcctg
     3721 ccggtaaatt accgtaatac gaaacgcatt ctgcactttg cttatgcatt ctcccgtgag
     3781 tattttgaga agcatcagaa ccgtgaaata ccgtttgttc agccacaggc gggcggtgaa
     3841 gagggcacag agccggaaat tctgcgttgt gcttctgaga gtgatgaggc agtgcaggtt
     3901 gtgggctggc tggagaaacg ctatacactg tgcggtcact ggagtgatat ggctgttttg
     3961 tgtcctgcag agttttcagt gaaacatctg aaagaggtca tgacacaacg cgggatacct
     4021 tatgccacct gctttgacag tgaaggtaag aagaaatatt cgcgacgcaa ggatgttgtg
     4081 catttactga cctaccagag cagtaaggga ctggagtttc cgtatgttgc ggttatcaat
     4141 gcttctttcg ttcactcagg tgctgcggat gagtctgaag ttattccggc gctttatgtt
     4201 gcgttcaccc gggctacacg ggaattactg gtgacctgtt atcgtgaaaa cagtatcagt
     4261 cgtcatcttg aggattttgt ataagttgtg ttttattatt gctgatatgt aatcattcgt
     4321 tttttgataa tctaagatgg gtatttagcg agtattcaga gttgaattaa tatcccccat
     4381 gtttttgatc tgtcaatagc tgtccattac atggggtgat catatatcag tcggtaatta
     4441 ctggataaag tcacccccta aatgattact ttattgtcca tgccggacta aagttttcct
     4501 cctcaaatag ttctgttaac ccacttattt gttttaatct cagtacctca tctgtatcca
     4561 tgcccagttc actacctatt cggagatcat cccatcccat tcgggaaaga tctcggacga
     4621 tatcggagag cgcgtctatc tggtgctttc ctctggccct gttatgcctg atggtcgagg
     4681 cgatagagta agctttaaag ttattatcat gcttaatgca tgtgacaggt aaatatcctt
     4741 ttagtttttt ctctagtttt gttgtgcttt tcccaagcat ttgtctgtga aatccgtcaa
     4801 taacggtgta tttccccttt tgagctaagg tgacaacagg ttgagtgaat ccatcaacca
     4861 acagggagtg tgttaataac cttttttcgg agggagccat gacgttagga ttatagtcgt
     4921 ttgcttcaat tagattgttt tttatccata taacacaatc aacaggttct gcgtaaaaag
     4981 gactactcct atgtaactca atctttattt tattaattgc atcaatgcgt tcatcttctt
     5041 tcttaagaga attaaggtat acatttattt catttatgat ttgttcaatg ctcatgtgtt
     5101 tctatttctt ttaatttgta tatttttctt gttttaagat gagttgttct tctttctcta
     5161 ctttgtgtta atatataagt tgtaaggttg ttttgtgtt
//
LOCUS       DAZUCC010000275         5113 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZUCC010000275 DAZUCC010000000
VERSION     DAZUCC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.277"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..259
                     /locus_tag="ACHY09_004633"
                     /pseudo
     CDS             3..259
                     /locus_tag="ACHY09_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(281..552)
                     /locus_tag="ACHY09_004634"
                     /pseudo
     CDS             complement(281..552)
                     /locus_tag="ACHY09_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(549..2309)
                     /locus_tag="ACHY09_004635"
     CDS             complement(549..2309)
                     /locus_tag="ACHY09_004635"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFS3887501.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGMTDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPRITRPIAVNILLDLYKTAERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPSADTWRILSGGPALYSLDDISYRCLRYGMKVLG
                     TQSTGVRLGFETGFDSGSTLDYIYRNQPQGSTSLGRLIDKNYLNSVGWKGIRQRKIHL
                     QMLIQQAVARLTEQGSSVRIVDIAAGHGRYVLDALQGETAVSEILLRDYSELNVAKGQ
                     EMIASRGMAGRARFEQGDAFNRNELAALEPRPTLGIVSGLYELFPENGPVRDSLAGLA
                     AAIEPGGILIYTCQPWHPQLKTIAWSLTSHKNGKAWVMRVRTQGEMDALVRDAGFEKC
                     TQLIDEMGIFTVSMAVRRQP"
     gene            complement(2302..2943)
                     /locus_tag="ACHY09_004636"
     CDS             complement(2302..2943)
                     /locus_tag="ACHY09_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS3887502.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTER
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQRLTALLQPSQGNHPHD"
     gene            complement(2947..3864)
                     /locus_tag="ACHY09_004637"
     CDS             complement(2947..3864)
                     /locus_tag="ACHY09_004637"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS3887503.1"
                     /translation="MTLLEKSLIVIFSLLLIATVINGLLVWLRPSKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPADGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            complement(3861..4466)
                     /locus_tag="ACHY09_004638"
     CDS             complement(3861..4466)
                     /locus_tag="ACHY09_004638"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS3887504.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIVAWNNVIWGVATLLLLWTIFNRCRSALREEGVK"
     gene            complement(4524..4709)
                     /locus_tag="ACHY09_004639"
     CDS             complement(4524..4709)
                     /locus_tag="ACHY09_004639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887505.1"
                     /translation="MLTQLHRVLYRCPGHYVYGSATQSTGTGTGSLASRNTSQSPLPA
                     QQAGFPRPELYSLSVCQ"
     gene            complement(4950..5102)
                     /locus_tag="ACHY09_004640"
     CDS             complement(4950..5102)
                     /locus_tag="ACHY09_004640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFS3887506.1"
                     /translation="MAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITILCVYILN
                     KKYAIE"
BASE COUNT         1352 a         1402 c         1266 g         1093 t
ORIGIN      
        1 ttatgccaat gaagcttgta aagaagaatc cccggagcaa agtgattcgg gagcttagct
       61 gtgagcaaga gatggcagta aaggcggtct gcggactgaa aaaacagcaa ctacggcact
      121 acatgccttg gccaatgatc ttctccggga aatgcgtgag tatgatgtat ggttacagtg
      181 tgactgtatt ccgggagatt cgccgaccat gaattttgct gctcttaaaa acaacacggg
      241 aactttttat ctgtccagtt tggatataga taattattgt actaataagg gaagccaaca
      301 ccagccgaca gaccagacgc cgctgctcat tcagtgaaac gcagataaac agtaaagcaa
      361 atccagactc cagtacggaa taatcgtcag cggtattaac gggatccggt gctcccagtc
      421 aaacaccagg ctgctgatgt tttctctccc ggcggtaaac tgattcgtca tgccataagt
      481 aagaaagaag aatggggcca gcaccattag ccagccgatt ccctgaataa tcaccgcacg
      541 ccgcgttgtc atggttgacg tcgtaccgcc atagagaccg tgaagatgcc catttcatca
      601 attagttgcg tgcatttctc aaaaccggcg tcacggacca gcgcgtccat ctcgccctga
      661 gtacgtacgc gcattaccca ggctttgcca tttttgtgac tggtgagtga ccaggcgatg
      721 gtttttaact gagggtgcca gggctgacag gtgtagatca gaatgcctcc cggttcaatc
      781 gctgcagcca gcccggcaag cgaatctcgt accggcccgt tttccgggaa cagttcatac
      841 agaccggaca cgatccccag cgtcggacgc ggttccagtg ccgccagttc attacgatta
      901 aaggcatccc cctgctcaaa tcgcgcacgg cccgccatac cgcgagaggc tatcatctcc
      961 tgacctttcg ccacgtttaa ttcgctgtaa tcgcgcagaa ggatctcgct taccgccgtt
     1021 tcgccttgca gggcatcaag cacatagcgt ccatgcccgg cggcaatatc cacgatccgg
     1081 accgagctac cctgctctgt taaccgagca acagcctgct gaattaacat ctgcagatga
     1141 attttacgct ggcggatccc cttccagccc acgctattca ggtagttttt atcaatgagg
     1201 cgtcccaacg aggtgctgcc ctgaggctgg ttgcgataga tgtagtctag ggtactgccg
     1261 gaatcgaaac cagtctcaaa tcccagacgc acccccgtgg actgcgtgcc cagcactttc
     1321 atcccgtagc gcaaacagcg gtaagagata tcgtccagcg aatacagcgc agggccaccg
     1381 ctcagtatgc gccaggtatc ggcgctgggg ctccagcgat cttcctggct gtaatcaaaa
     1441 cgctgcacgg gacgggcata caaggtatca ataaaactgc gcatcctgtc aaatgccaga
     1501 tgccgctctt tttcgcctaa ggtgtcatga taaaatcccg gcagtacatg cagttctttc
     1561 tgcggatttc gcaggttctg ataaaaatca agctgcggct ggcggtgcac aacgtaatct
     1621 tccccggaga ccagcaactg cacaggtagc gtaatggcgc tggcatcgct gacgactcgc
     1681 tcagccgttt tgtagagatc aagcagaata ttcacagcaa ttggtcgggt aatccgcgga
     1741 tccttattga agcttgctac ccgctcggga tcgtgcgtca gatacttgcc tttcacatac
     1801 gagttgacga agaaaagacc gcgcaggcga tacatcaatt ccagcccctg gcgagcaaac
     1861 ggcacataca atttgacctt aaacgccggt gatgccaaca ccaggccacg aatgtccggg
     1921 gcataatcat gcgcccaggt tgccgccaga accgccccga cgctttgcgc cacgacgacc
     1981 acatccgtca tcccagcttg gctatctgcg gcggcgaaac gcacaaactc gtcaacatcc
     2041 tgcaccgaac gcacgagcga cggactgtaa ccacgcagac ccggtgagcg accatgacct
     2101 cgggcatcca tggcataaaa cgctgtatcc ggcatcgcca gctcatcaac aatgtgttgc
     2161 aaacgcccgg agtgctcatg cccacgatga aacaggacca ccacttttgg cgttgaactg
     2221 gcttcttgtg ctggccagtg gcgataaaac agctctgtat tatcactggt cagaaactgc
     2281 cgttcatcgg gtatgcggga attagtcatg gggatgattt ccttgcgagg gttgcaaaag
     2341 cgccgttagt ctttgctcca ggtcgtccat aaacgttttc ttctccggat gcgtacttag
     2401 cgcatcgcca atatttacat caataaacag cggcaaaggg atacgattac ctttcgccat
     2461 cacacgctca gtaccgcgca acccaacggg gataatgggc acctccggca tgcgctgact
     2521 caaatgccac actccggatt tcaatggcgc aagtacgcct ggctcaccgc gcgtcccttc
     2581 cggaaacagg atcagtgttt taccttcacg cagcgcctgt tcacattgcg ccagcggatc
     2641 ggtataaccg ccctttcttg aaataggaat gatgttaagg atcttcagcg caaaccaagc
     2701 catgcgttta tcccgcagaa aataatccgc ggcagcaacc ggatgaacag aagcctggta
     2761 acgcagcgga aaaagcgaaa gcaaggcgaa aacatccata tggctgttgt gatttgccac
     2821 gattatcgaa ggcccgtgct tcggcagtcg ctcgcggtgg cggacacgca gaccaagcca
     2881 cagccagatg accggccaga cgatacacag cgtaaacagg gtacggagta gtcgtgtcat
     2941 cataggttag tagcagaaat agcgaacaaa atggaagaag accggtgcgg taaaaatcag
     3001 cgaatccagc cgatcaagga tgcctccatg gccgggtagc agcgtaccac tgtctttgac
     3061 gccgatatca cgcttgattg ccgacatcac cacgtcgccg caaaagccag taacgccgat
     3121 aataaccccg gctaacagtg ccatcggcca gctcagggga gtcaacagtg gccccaggac
     3181 tgttgccgcc agcgccgtcg ttgccacccc acccagcaat ccctccaggg ttttgtttgg
     3241 gctgaccttc ggtatgactt ttatacgacc aagcgacttg ccccacaggt attgcgcgat
     3301 gtcgttaaat tcggtcaggc cgaccaaaaa cagcaccaac agcgcgctgg tttgcattcc
     3361 atccgccggt aacaccagta aaaacgcgac atggctgaag gcaaatacgg tggtcatcag
     3421 actccagtgc aactgggaaa cggtacgtaa aaagccctgt gtatccccag caatcaccat
     3481 tcgcgccggt aaaaaaagga acacgtagac cgggataaac accacaaaca tgccgtacca
     3541 gccgatacct atcagccagt agttaagggg aattgcaata aacatccata acagcggcat
     3601 gcggtcggac tggcgggaag gcaccagcgt caaaaactct tttagcgcca taaagctgac
     3661 cagcgcgaag aacgtcagtg ccagccagct aggacttaat agtgcgaagg aaaacaggac
     3721 gataatcacc caccaggtgc gaatacgtaa tgtcagctcg cgccagtctt tacttggacg
     3781 cagccacacc agcaacccgt tgattacggt ggcaatcaac agcagggaaa aaataacgat
     3841 cagtgatttt tcaagtagcg tcatttcaca ccctcttcac gcaatgcgct gcgacagcga
     3901 ttaaaaatcg tccataacaa caacagtgtt gcgacacccc atatgacatt attccaggcg
     3961 acaatctgcg gccagatcga cagtgacagc ccccatgcac caaacaccag cgctctgtcg
     4021 cttttgccca gcggaccggc atagctgcgc acgccgttaa tcgtttgcgc cagcacgccg
     4081 caaaactcgg tcatcagcgc acaaaacacc acgatcagca ccagcggtat actgctctgc
     4141 gggagtagca taaagggcag ataaagcgag atgtcggaaa ttacatcacc cgtctcattg
     4201 aggatggccc ccagacgcga tctttgatta cattcacgcg ccagcatacc atcgagcgca
     4261 ttgagcgcca ttcgtacaaa caacaccacg ggcagaagga cgaataatcc gggctgagga
     4321 tgaataatga gcaccacgcc ggtgataagg gaaagcacca tcgcgcacag tgtaatgtga
     4381 ttagcggtga cgccgcgcct gttcagccag aacatcagcg gtcttaacag agcctgaaac
     4441 gagggcttta tctggtaaag ggtcatcatc actccttaat aagcatattt gacggggttg
     4501 agtctggcct tttattgggg ctgtcactgg cacactgaaa gtgaatacag ttccgggcgc
     4561 ggaaatcctg cctgctgcgc cggtaaaggc gactgactgg tattacggga ggccagagag
     4621 ccagtacctg tgccggtaga ctgtgtggct gaaccataaa catagtgtcc tggacaacgg
     4681 tacagcactc tgtgcagctg tgtcagcatg cgcttttctt taattatgcc cagtcatgtg
     4741 cggaggcaag gatttcaaaa tatccgttac atttgttgca tgatgcctta tcgctggtcc
     4801 gggctattgc tttacttctt ttaaatcagc tctgatgaac attttttacc actttatcgc
     4861 attaaggtta tgtcattcat cgtctggcat cacgtttttg gttatggtgt cagggtgtac
     4921 cctaccgcga taacgcaacc ctaataaagt cattcgatgg catatttctt attgagaata
     4981 tatacgcata aaatagttat aaaaacgcct agtccctgaa taaatgatat atcttcctgc
     5041 aggaagaacc aggacaacag caatgctacc agaggaataa aatagagcat tgctcctgct
     5101 atgtgaacgg gaa
//
LOCUS       DAZUCC010000276         5032 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZUCC010000276 DAZUCC010000000
VERSION     DAZUCC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.278"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..729)
                     /gene="aroD"
                     /locus_tag="ACHY09_004641"
     CDS             complement(<1..729)
                     /gene="aroD"
                     /locus_tag="ACHY09_004641"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS3887507.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDIIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLR"
     gene            complement(760..1626)
                     /gene="ydiB"
                     /locus_tag="ACHY09_004642"
     CDS             complement(760..1626)
                     /gene="ydiB"
                     /locus_tag="ACHY09_004642"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFS3887508.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1638..2903)
                     /locus_tag="ACHY09_004643"
     CDS             complement(1638..2903)
                     /locus_tag="ACHY09_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3887509.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFATFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(3130..4344)
                     /locus_tag="ACHY09_004644"
     CDS             complement(3130..4344)
                     /locus_tag="ACHY09_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3887510.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALLLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(4444..4800)
                     /locus_tag="ACHY09_004645"
     CDS             complement(4444..4800)
                     /locus_tag="ACHY09_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFS3887511.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTDDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTVFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4936..>5032)
                     /gene="rprA"
                     /locus_tag="ACHY09_004646"
     ncRNA           complement(4936..>5032)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHY09_004646"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1461 a         1152 c         1151 g         1268 t
ORIGIN      
        1 acgcaaatca tttaccgaga tttgccctgg cgcagaggct ttttttaccg caccaaaagt
       61 tgccgccgag ccaaatactt caccagccag acgagaaatt acgccagttt ttgccatcga
      121 catcgtgatg attggacgat cggcatactg ctcctgcatc tccagggtcg cagcaagcaa
      181 cgtcagcaca tcgctggtac tttgcggcat cagcgcaatc ttaggaatat cggcatcgaa
      241 ggactgcatt ttgcgcagac gggcaatgat ttcttcggct tccggcgttt tatggaagtc
      301 atggttggac atgacaactt tcacatcatg cgcgtgggcg taggcgacgg tttctttaac
      361 ctgatcatcg ccggtaaata actccagatc gattatatca accaggccgc tgtcgatggc
      421 tgcacgattg agtgcaatat aagcctcggt ggaaatcgcc tgctcgccgc cttctttggc
      481 actgcggaag gtaaacagca gcggtttttc tggcatggtt tcacggagaa tttttgctgc
      541 cgctatgaca gactccacat tggagagatc ggcatagtgg tccacacgcc attccagaat
      601 atcaaagtcc gcttcacgat aggcgagagc ttcggatttc acgctggcga tatctttcgc
      661 catcagcgag acgatgattt taggtgcgcc cgtaccaatg acgagatctt ttacagttac
      721 ggttttcatt ttttaccctt tctgcacgcg gtcagcctgt caggcaccga accccatgac
      781 ctgtttaaca tattccagag ggaaatcttt gccagtccat aacgtgaact gttcagcccc
      841 ttgccacaac aacatgccgt atccatcaat cgttttgcaa cccgcttgtt gcgcctgctg
      901 caataacttc gtcatatgag ggttatacac gcattcagtg accagaagtc ccggatgtaa
      961 cagactgata tcattaacca atgattcatt ctcaaggggt ttcataccca ctttggtgcc
     1021 attggttaaa atgtcggcgg aagccagggc ttctgcaaag gcttgctgat cggcgagatc
     1081 ggtgactgtc acgacgcaat cggtgttttc gttaacccgc tgcgcgaagg cgagagcttt
     1141 atcgaagaac tcatcccgac ggttaaagag tttaatttct tttaaacctt caattgcccc
     1201 ctgcgcgcca attgccgttg aggcaccacc ggcccctaat agcaccatcg ttttgccttt
     1261 gatatcaaaa ccgctctctt taatggcgcg aatatgaccc gtgccgtcgg tgttatagcc
     1321 acgcagatag ccatcatcat taacgatggt gttgatggct cccaccagtt tggccgctgg
     1381 cgttaattca tcaacatatt cacacgccag ttgtttgttc ggcattgata caccagttcc
     1441 gcgcattttg agggctttta atccttcaat tgctccggga aaactatcgt tatccacttc
     1501 aaaggccata taggtaaatg gcaatcccgc tttttctaag gctttattct gcatttcggg
     1561 cgataaactg tggcggatgg gataggccat caacccaatc aattcgtatt ttgcggtaac
     1621 atccataatt aactccttta acctctatgc ttaatcgtgt ttaaccgggt gctaaattta
     1681 tgctcgccaa accgcacatc attttcagga ataatgaaaa cgcggtaata gcggataaaa
     1741 acaataattg cggtaatcag ggccagcagc gcgaaagtaa aatcgagaac aatgatatat
     1801 tgcaggccga tgttcgacag ataaccggta atcagcggaa taacaaagtt agccagtcca
     1861 cccatcatca tatatatact ggtgactttg gctttgcttt ttgggaaaaa ctctgacatg
     1921 accgaaacgc cgagctgtaa aatgccgcca gctgctgaga aaccgataac aaaggctccg
     1981 gcattacaca ccaatggaga agggtacagg tagataatgg cagctgttat tgttgccagt
     2041 gcagagttaa atacattagc ccagatgggc cggaccattt ttttcagcag ggcggcaaaa
     2101 ataaagacac agaccagcga acccatactg taataagaga tggtttttaa tgcctcagct
     2161 tctgacatac cagcaaaagc catcgcatat ttgggcatcc acaccacaat cacataaaag
     2221 gtcgcgaatg cggctacacc gaacagtact gatgaaacac cttccagcca gactaacggt
     2281 ttgctgttca tttgcggtaa ttcattagct acgctggcgt ccaccaactg gctggggaat
     2341 ttgcttttca acagcatcag cgtgatcagt acaaacagaa tacccggaat aatcagccca
     2401 tagccgtacc agatgttatt gagcaacata tagctcacca gcattgggta gaacatttgc
     2461 ccgaatgaca ccatcgcttt aaccagtatg accgccgaac cagaggcttt cggaaagcat
     2521 tccataagcg cggggtagcc acccgtatcc agcgctgagt tagcgatacc tacgcacact
     2581 gccagacagt aggcgagagt taaattcggg caagcaggaa taccaaagaa gaatagcaga
     2641 tacattatta ctgccattaa tatcaccgcc cgacgaccaa acttatcgga gatcacaccg
     2701 aagaataaaa tactgatcaa tcgccccaat ccgataccgg aaattaaata ggcaatgccc
     2761 gcgttgtcag tggaaaactt ttctgccaaa gatgtcatgt tttgggcaag cgtaataaca
     2821 ctaatgccgt gcaggaaata gctgaagtaa atacaaagaa cagccaggat aaatggcgtg
     2881 ctgaaagcct tattttgaga cattttcaca gctccttgct gaaagataat ttcgttaccg
     2941 tacgtattgt tctccgacgg tttacataat tactatactg aagataattt tccctgggta
     3001 catggcataa agtttatgtt tccaggaata ttgcatgtca tagtttgttt ttgatcgcga
     3061 ttttccttag ttagcgcaga aagagttatt tatccgtaag atcttgcatg acctgaaaag
     3121 aatattgcat tacccacccg gagcgacatt ttcctttagc gagtgatgcc gcgtttcttt
     3181 gcgactgcgt aagccaataa acagtgccag tataaagccg atggcagcga tggcggtatc
     3241 gaaccacata atatcggcaa tacttctttg cgaaagatga gccgtaatca acggaatagt
     3301 aaaggttgca atgctgcccg cactgtaata gatccccgtt gcttttcctt tagcgtaggg
     3361 aaaacgttcc gccattaacg tcaggccaat ttgtacaaca ccgcctgcgg aggtaaaacc
     3421 gatgacgaaa gcaaatatta tcaccacata aaatgtggga tgcaggcaga cggtgagcag
     3481 ggcgataaat gagataaagg tgtacagcat cagtaatgtt gttgggcgaa cggtattacg
     3541 aatgagtgga gcggtaataa atacacaaag cagcgaaccc acggtgtaga tactgagtaa
     3601 tttgatcgac atggtgtatg acatgccggc gacaaattgt ccgtattgtg ccagccactg
     3661 gctaaccaga taaaacgttg ccattgagat atagccatat agggaataac tggctaaatc
     3721 gataattgaa cagcgatgtt ccgtagagct ggtggttttc tttatgacag gtaagtgacg
     3781 acccggatgg ggtgggaacg tacaacggta taaaagcaga gcgttaataa acataatgcc
     3841 tgcggcaatc ataaaggacc aaccgaacca cagttcagcc cacactaaca ggctgataat
     3901 tagcggtaat aaaaattgtc cgctggaaac aaatgcttta attaaaatat tggctgtacc
     3961 cggtgagcgt ggaaaagctt ccatcaaact gggataggtg cctgcatcga gaaaactgtt
     4021 tgccattccc gccagaaagc caaaaacata agcgataatg atgttattgg tgtgcaggat
     4081 gccaaaaaag aaggccatat agcagcacat cccgagcatg ataaaagggc ggcgaccaaa
     4141 gcgatcggat aataatcctg caaaaagcaa gacacttaat cgaccaatgc ccagtgatga
     4201 gataactatc gagacgcccg cggcattagt ctgccaaagc gtctccagcg aggccatatt
     4261 caggctcatc aaaatgacgc ccataccatg caccaggtaa ttaaaataca acccaagtgc
     4321 ggtagggtaa tagggatttt tcataggttc tacttagcaa aggtgcttta aaaagtgtag
     4381 agatgttgtg tcagtatccg ttcattattg ttgtaacaaa gacaattcct ttgtgtggag
     4441 aaattaacaa agccgctcca gatcgtgtgc atataattca gcggctaccg attgatagat
     4501 cttccagtca ataaaattgc catcagggta gacttgttga aacacggtta agtcggggaa
     4561 aaaacgcatg gtgttgttgc cgatgcggtt atttattttc tctatgatgg catgaagatg
     4621 tttttttcgt tgctgacgca tagccaacag ttgctcgact acacgatcag gaatggcgca
     4681 tttgccattt tcccactgac gccagctttc actgtcatcg gtctgggcaa tccaggtcgc
     4741 gcattcatca atagtcatgg caaaaatatg gcgaagtgcc tggagttcat aagcgttcat
     4801 cgtgtaatgg ctgcatgaaa taaacaataa aatataaata acattatcag cctgctgacg
     4861 gcttgaagag agtcacagta tcttgtgcaa cattattgga aagcgtgagg ggcgagatag
     4921 caaagcggaa aaatgttaaa aaaaagccca tcgtgggaga tgggcaaaga ctacacacag
     4981 caattcgttg tttcactcag gggatttcca tgcttataaa tcaatatgtt ga
//
LOCUS       DAZUCC010000277         4993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZUCC010000277 DAZUCC010000000
VERSION     DAZUCC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.279"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..189
                     /gene="rrf"
                     /locus_tag="ACHY09_004647"
     rRNA            74..189
                     /gene="rrf"
                     /locus_tag="ACHY09_004647"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            204..279
                     /locus_tag="ACHY09_004648"
     tRNA            204..279
                     /locus_tag="ACHY09_004648"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Thr,seq:ggt)
     gene            319..434
                     /gene="rrf"
                     /locus_tag="ACHY09_004649"
     rRNA            319..434
                     /gene="rrf"
                     /locus_tag="ACHY09_004649"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(497..1255)
                     /gene="yhdZ"
                     /locus_tag="ACHY09_004650"
     CDS             complement(497..1255)
                     /gene="yhdZ"
                     /locus_tag="ACHY09_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS3887512.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1263..2366)
                     /gene="yhdY"
                     /locus_tag="ACHY09_004651"
     CDS             complement(1263..2366)
                     /gene="yhdY"
                     /locus_tag="ACHY09_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS3887513.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKNQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2376..3557)
                     /gene="yhdX"
                     /locus_tag="ACHY09_004652"
                     /pseudo
     CDS             complement(2376..3557)
                     /gene="yhdX"
                     /locus_tag="ACHY09_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3625..4650)
                     /locus_tag="ACHY09_004653"
     CDS             complement(3625..4650)
                     /locus_tag="ACHY09_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3887514.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1372 a         1288 c         1194 g         1139 t
ORIGIN      
        1 gagagaagat tttcagcctg atacagatta aatcgacagg tcataacgag acgtgttgat
       61 aaaacagaat ttgcctggcg gccttagcgc ggtggtccca cctgacccca tgccgaactc
      121 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
      181 ctgccaggca tcaaatttag cgtgctgata tggctcagtt ggtagagcgc acccttggta
      241 agggtgaggt ccccagttcg actctgggta tcagcaccac tttttaggtt aaagttcggc
      301 agattagaaa agaatttgtc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
      361 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
      421 gggaactgcc agacatcaaa tcaagcgaaa ggccatccga aaggatggcc tttttgcttt
      481 tcgaactaac attcaattaa tggatcacct gcgataaaaa tgccctcgta cgctctgatt
      541 taggatgcgc aaaaaactca tccggtgcag cttgttccac tatctcccca cgatccataa
      601 aaatcaccct gtcagcgacg gttcgtgcaa accccatctc atgtgttaca cacagcattg
      661 tcatacccga ctgcgccagc ccaatcatcg tatccagcac ctctttcacc atctcaggat
      721 cgagcgccga cgttggctca tcaaataaca taattttcgg cttcatacac agcgaacgcg
      781 caatggcaac gcgttgctgc tgaccacctg aaatctgtcc gggaaactta tgcgcatgtt
      841 cggcaattct cacccgctca aggtaatgca tcgccagagc ttctgcctct ttctttggca
      901 tcttgcgtac ccaaatcggt gccagggtac agttctgtag aacggtcaga tgagggaaga
      961 gattgaaatg ctgaaagacc attcccactt cctgcctgac gcgctcaata ttgcgaatat
     1021 cttcgttaag ctcgatgcca tctaccacga tccgtccctg ttggtgttct tccagatgat
     1081 taatacaacg aatggttgtc gatttaccgg aaccagaagg gccacacaga acgatccgtt
     1141 ctcccggttg cacggttaaa tttatatttt tcaaaacatg gaattgccca taccatttat
     1201 tgacgttttc cagcgtaatc atcgcgttag caggttgcag taaaatttgg ctcatagtgt
     1261 cctcaatgcg gtgtacgccc ggtgttaaaa cgtttttcca gatgttggct ataacgtgac
     1321 atgctgaaac agaagatcca gtagatcagt gcggcgaaaa catacccttc cgtcgacata
     1381 cccaaccagg cgggatcaac ggttgcctgc tgaacgctac tgaaaagatc gaacaacccg
     1441 atgatgatca ccaggctggt atctttgaag agtgcgatga tggtatttac cagcccagga
     1501 atcaccagct tcaacgcctg tggcagaata accagcccct ggtttttcca gtaacccaac
     1561 gccagcgact ctgccgcttc atattgccct ttaggcagcg cctgtaatcc gcctcgcacg
     1621 acttccgcaa catatgctga ctgaaacagg atcacgccaa ccagcgcgcg gatcaatttg
     1681 tcgatactgg tgccttctgc cataaacaac ggcagcatga ccgaagacat aaacagaacg
     1741 gttatcagcg gtacaccgcg ccagaactcg ataaaaatga ccgataagat acgcacaatc
     1801 ggcatatggg agcggcgacc taacgccagt aagatccccc acggcagcgc ccccgcaatc
     1861 ccaactgatg caataattaa agttagcgtc agcccgcccc attgccgggt ttcaacccgc
     1921 tcaagaccaa gaaacccgcc atacatcagc caccagacaa tcagtgggta aatcaccgcc
     1981 cataccgcaa tatagcgacc gcgatgcggg agtattttcc agaacattgg tgcgatcgac
     2041 acaagcccaa tcagtaatgc gaggttaatt cgccagcgtt ggtcgtgtgg gtaaagccca
     2101 tacataaact gaccaaatcg ttcgtggatg aagacccaac aggcaccggc ttttgtgcag
     2161 tcggtacgcg ttgagccaac ccagttagcc tgcaaaaatg cccagttcag caacggtgga
     2221 atcagttccc acatcaacca tatgcagcca atagtcagca ggctattgct ccagctggag
     2281 aacagatttt ttcgcaccca caccatcgcg cggcttgagt tatggctcgc cgggcgcggg
     2341 ggatgagaca gcaatacttt tgtcatcatg actccttagc gttcaacgat cgcgatgcgg
     2401 cggttataga tattcatcag cagcgagata gtcaggctga taatcagata gaccgacatg
     2461 gtcatggcta tcgtctcgat ggcttgcccc gtctggttca gcacggtgcc agcaaacagc
     2521 gaaaccatat ccggatagcc aatagcggcg gcaagagagg agtttttgac gatgttgaga
     2581 tactggctgg ttaacggcgg aataatcact cgcagtgcct ggggaataat gacctggcgt
     2641 agcgtaaccg gattgggtaa tcccagtgac cgagccgctt catgttgacc ataaggcact
     2701 gcctggatcc cagcgcggat aatctcggcg ataaatgcag aggtataaac cgaaagcgcc
     2761 agcgttaagg ctgccagttc aggaattaaa accatcccgc cacggaagtt aaagcctcgt
     2821 agggctggaa catcccagtg tagtgctgcg ccaaaaagcc attgcgccag taaaggcaaa
     2881 cctatgatca acataacggc gaccggccag gttcggcgca attgtccggt ctttatctgg
     2941 tacgttttat taaaacggaa tagtccaaca gaaatggcta tagcaataac aacagtcacg
     3001 ataaacgcaa taaatccgtc tcccagctgc ggagatggaa tataaagccc acgattgctc
     3061 aaaaaggcca gatcaaacgc actcactgcc tggcgtggtc cgggcaaatt gcgcaacacg
     3121 gcaaagtatc agaagaagat ttgcagcagc ggcggaatat tacggaagat ctcaatataa
     3181 attgtggaaa gctttcgtag cagccagtta tccgaaagtc gcgccagacc gataaagaag
     3241 cccagaacag aagcgaatac aatacacaat gcagaaacca gtagcgtatt gagtaagcca
     3301 acaatgaaaa cgcgtccgta ggtatcaccc tgctggtaat cgatcaaatg ctggacaata
     3361 ccgaagccag cgccgcgatc cagaaaggca aaacctgaag taatgccacg attactgaga
     3421 ttcgtcacag tgttgtgaaa tagccaacca acaattccga caacagcaac aacggcaaag
     3481 atctggaata accaggcgcg aaccgtaggg ttggcaaagg agagtgagcc tttaacggtt
     3541 gagcggcgat gagacataga aacctcggaa agcataacgc tgaagagagc gccgccgtag
     3601 cgacgctcgt tacctcacac cgacttaacg cacgggcggc gcgtactgaa taccgccgtt
     3661 attccagaga ttattttgcc cacgtttaat tttcagcggg ctttctgaac ctacgttacg
     3721 ctcgaaaatt tccgagtagt tacccacctg tttgatgatg ttaaaggccc atttattatc
     3781 cagcttcaga tccttgccgt aatcgccttc tttacccagc agatgtgcca tatcaggtgt
     3841 tgctggatta gccgcttttt catcgacgtt ctgggaattg atgcccatct cttcagcatt
     3901 cagcatggcg aaaagcgtcc agcgtacaat cgagaaccat tcatcatcgc cacgacgaac
     3961 taccggccca agtggttctt tagagataac ttccggtaag acaatccatt cagccgggtt
     4021 gcttaatttg atgcgcaggg catacagttg tgattgatcc gaggccagcg tatcgcagcg
     4081 accagattcc agtgccttcg ctgattcgtc agagcgatcg aaagtcactg gtgtgtactt
     4141 catattgttt gccttaaagt aatcggcaac gttgagttcg gtatcagtac ccgcctgaat
     4201 acagacggta gcgccatcca gttctttcgc gctttttagc cccgctttat cgtgcgtcag
     4261 gaagccaatg ccgtcgtaat aagtgacgcc agtaaatgcc attcccatcc cggcatcgcg
     4321 agatgaagtc caggtcgtat tacgggagag caaatccacc tcccctgact gtaaagcggt
     4381 gaagcgttct tttgcagtga gcggggtata tttcactttc gtatcgtcac cgaatacagc
     4441 agcggcaaca ccacgacaaa catctacatc aatacctgaa aacttaccgt cagcatcggc
     4501 ataagagaac ccaggtaatc catcactgat cccgcattgc acaaaacctt ttttctgaac
     4561 ggcatcaagc gtcgcgccag catgcgcctg atttgcaacg gcaagcagca cgctggcggc
     4621 agccagtgtg gctatcatca tctttttcat aatgcttcct gtgaggcgaa aattatcgtt
     4681 ataggacgtt tgggaaacgt cttttacctg tctgtggcca tcctcttata agcaaaagta
     4741 gtgccagctt ttgcgcacga tttcgcgtcg caataagctg agtgtagaca ggaaataata
     4801 aaaactgcgc cccgcaatgg tgcgaaaatg aaacatgcgc cacattttgg cgcaaaatat
     4861 ttacgcgttt tttactaagc agaataatga ttgttagcgt gagtatattt ctggtgggat
     4921 gtgtgaaagt taaattgatg cgttatttcg aaagcagtga aataacgtga tataacatgc
     4981 taaatcagca ggg
//
LOCUS       DAZUCC010000278         4924 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZUCC010000278 DAZUCC010000000
VERSION     DAZUCC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.280"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            123..1457
                     /gene="garP"
                     /locus_tag="ACHY09_004654"
     CDS             123..1457
                     /gene="garP"
                     /locus_tag="ACHY09_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFS3887515.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1473..2243
                     /gene="garL"
                     /locus_tag="ACHY09_004655"
     CDS             1473..2243
                     /gene="garL"
                     /locus_tag="ACHY09_004655"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFS3887516.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2273..3163
                     /gene="garR"
                     /locus_tag="ACHY09_004656"
     CDS             2273..3163
                     /gene="garR"
                     /locus_tag="ACHY09_004656"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFS3887517.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3260..4405
                     /gene="garK"
                     /locus_tag="ACHY09_004657"
     CDS             3260..4405
                     /gene="garK"
                     /locus_tag="ACHY09_004657"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFS3887518.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4438..4814
                     /gene="rnpB"
                     /locus_tag="ACHY09_004658"
     ncRNA           4438..4814
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHY09_004658"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1151 a         1172 c         1409 g         1192 t
ORIGIN      
        1 tattattctc gcgctgctta tactaattcc cgagttcatt gtcgtaacgg cataaatgaa
       61 actcataaat aacaccttcc ccataataat aaaaatcagc ataagtaccc gaggtaaata
      121 aaatgatgct ggacaccgtt gacgaaaaaa agaaaggcgt gcatacccgc tatttaatat
      181 tactgattat ttttattgtt accgccgtta actacgccga tcgtgcaacg ctgtctattg
      241 ctggtaccga agtggcaaaa gagttgcagt taagtgcggt ttcgatgggt tacatcttct
      301 ctgcttttgg ctgggcctac ttgctgatgc aaatccccgg cggctggctg cttgataagt
      361 ttggttcgaa aaaagtttac acctacagcc tctttttctg gtcgctattc accttcctgc
      421 aaggctttgt tgatatgttc ccgctggcct gggcagggat ctccatgttc tttatgcgct
      481 ttatgctggg gttctcggaa gcgccatcat tcccggcgaa cgcccgtatt gtcgccgcct
      541 ggttcccgac gaaagaacgt ggtactgcct ccgccatctt taactcggcg caatatttct
      601 cgctggcgct cttttcgccg ctgcttggct ggctgacttt cgcgtggggc tgggagcacg
      661 tctttaccgt tatgggggta attggttttg tgctgacggc gctgtggatc aagttgattc
      721 ataacccgac agatcaccca cgtatgtctg cggaagagct gaagtttatc tcggaaaatg
      781 gcgcggtggt cgatatggac cacaaaaagc cgggcagtgc ggcagcaagc ggacccaaac
      841 tgcattacat caagcaattg ctctctaacc gcatgatgct gggcgtattt ttcggacaat
      901 attttatcaa caccatcacc tggttcttcc tcacctggtt cccaatttat ctggtgcagg
      961 aaaaaggcat gtcgattctg aaagtgggtc tggtcgcctc gattccggca ctgtgtggct
     1021 ttgcgggcgg cgtgctggga ggtgtcttct cggattatct gatcaaacgc ggtttgtccc
     1081 tgaccctggc acgtaagcta ccaattgtgc tgggaatgtt gctggcttcc accatcatct
     1141 tatgtaacta caccaacaac accacgctgg tggtcatgct gatggcgctg gctttctttg
     1201 gtaaaggatt tggtgcgctg ggctggccag tgatttctga caccgcgccg aaagagattg
     1261 ttggcctctg cggcggcgtc tttaacgtct ttggcaatgt tgcctccatt gtcactccac
     1321 tggtgattgg ctacctggta agtgaactgc actccttcaa tgcagcactg gtttttgtgg
     1381 gatgttcagc gctgatggcg atggtctgct acctcttcgt agtcggcgac attaaacgta
     1441 tggaattgca gaaataagca aaggtataag cgatgaataa cgatgttttc ccgaataaat
     1501 tcaaagccgc actggctgcg aaacaggtac aaattggttg ctggtcagct ctctctaacc
     1561 cgattagcac tgaagttctt ggtttggctg ggtttgactg gctggtgctg gatggtgaac
     1621 atgcgccaaa cgatatttcc acgtttattc cgcagttaat ggccctgaaa ggcagcgcca
     1681 gcgcgccagt agtgcgagtg ccgaccaacg agccggtaat tattaagcgt cttctggata
     1741 tcggtttcta taacttcctg attccttttg tagaaacaaa agaggaagca gagcaggcgg
     1801 tggcatcaac tcgttaccca ccggaaggca ttcgcggcgt ctccgtttct caccgcgcca
     1861 atatgtttgg caccgtggcg gattatttcg ctcagtcgaa caagaacatc actattctgg
     1921 tccagataga aagtcagcag ggcgtagata acgtcgatgc tattgccgct accgaaggtg
     1981 tagacggcat cttcgtcggc cccagcgatc tggctgcggc attaggccat ctcggcaatg
     2041 cctcgcaccc ggatgtacaa aaagcaattc agcacatttt taaccgtgcc agcgcgcacg
     2101 gcaaacccag cggcatcctc gcaccagtcg aggctgatgc gcgtcgttat ctggaatggg
     2161 gcgcgacgtt tgtggccgtc ggcagcgatc tcggcgtctt ccgctctgcc actcagaaac
     2221 tggctgatac ctttaaaaaa taaccaccac cgcaataaga gagatgattg atatgactat
     2281 gaaagttggt tttattggcc ttgggattat gggtaaacca atgagtaaaa accttttgaa
     2341 agcaggttac tcgctggtgg ttgctgaccg taacccagaa gctatcgctg acgtgattgc
     2401 tgcaggtgca gaaacagcct ctacggctaa agcgatcgct gaacagtgcg acgtaatcat
     2461 aaccatgctg ccaaactccc ctcatgtgaa agaggtggcg ctgggtgaga atggcattat
     2521 tgaaggcgcg aagccaggta cggtattgat cgatatgagt tctatcgcac cgctggcaag
     2581 ccgtgaaatc agcgaagcgc tgaaagcgaa aggcattgat atgctggatg ctccggtgag
     2641 cggcggtgaa ccgaaagcca tcgacggtac gctgtcagtg atggtgggcg gcgacaaggc
     2701 tattttcgac aaatactatg atttgatgaa agcgatggcg ggttccgtgg tgcataccgg
     2761 ggaaatcggt gcaggtaacg tcaccaaact ggcaaatcag gtcattgtgg cgctgaatat
     2821 tgctgcgatg tcagaagcgt taacgttggc aaccaaagcg ggcgttaacc cggatctggt
     2881 gtatcaggca attcgcggtg ggctggcggg cagtaccgtg ctggatgcca aagcgccgat
     2941 ggtgatggac cgcaacttca aaccgggctt ccgtattgat ctgcatatta aggatctggc
     3001 gaatgcgctg gatacttctc acggcgtcgg cgcgcaactg ccgctcacag ctgcggttat
     3061 ggagatgatg caggcactgc gtgcagatgg tttaggaacg gcggatcata gcgccctggc
     3121 gtgctactac gaaaaactgg cgaaagtcga agttactcgc taatgacgtc gtgcccggat
     3181 ggcgtattgc aatccgggcc tggaatccag gccgaataag agaaacgccc tccggcgtca
     3241 tgtggtaaca ggcataggta tgaaaatcgt aatcgcccca gactcttata aagaaagttt
     3301 atctgccagc gaggttgcgc aggcgataga aaaaggattt cgggaaattt ttcctgatgc
     3361 acagtatgtt tctgttccgg ttgccgacgg tggtgaagga acggtggaag cgatgattgc
     3421 agccacccag ggggctgaac gtcacgcctg ggttacaggg ccgctgggcg agaaagtgaa
     3481 tgccagttgg gggatctccg gcgatggcaa aaccgcgttt atcgaaatgg cggcggccag
     3541 tgggctggaa ctggtacctg cggaaaaacg cgatccactc gtgaccactt cacgcggtac
     3601 tggtgagtta atcctgcagg cgctggagag cggtgcgaca aacattatta tcggcattgg
     3661 cggcagcgcc accaacgacg gcggcgcagg catggtacag gcgctggggg cgaaattatg
     3721 cgatgccaac ggcaatgaaa ttggttttgg cggcggtagt cttaatactc tgaatgatat
     3781 tgatatttcc ggtctcgatc cgcgcttaaa agattgtgtc atccgcgtcg cttgtgatgt
     3841 caccaacccg ttagtcggcg acagtggtgc ttcgcgcatc tttggcccac aaaagggagc
     3901 cagtgaggcg atgattgtcg agctggacaa taacctctct cactatgccg atgttattaa
     3961 aaaagcgctg catgttgatg tgaaagatgt ccccggcgca ggagctgcgg gcggtatggg
     4021 cgcggcgctg atggcgtttc ttggtgcaga actgaaaagt ggtattgaaa tcgtcactac
     4081 ggcgctgaat ctggaggaac atattcacga ttgtacgctg gtgatcactg gtgaagggcg
     4141 tattgatagc cagagtattc acggcaaggt accgattggt gtcgcaaacg tggcgaagaa
     4201 gtaccataaa ccggtgattg gcattgcggg tagcctgacc aatgatgttg gcgttgtaca
     4261 tcagcatggc attgatgcgg tcttcagcgt attgaccagc attggtacgt tggacgaagc
     4321 attccgcggg gcttatgaca atatctgccg tgcttcacgt aatatcgccg cgacactggc
     4381 gattggaatg cgcaacgcgg ggtgacaagg gcgcgcaaac cctctatact gcgcgccgaa
     4441 gctgaccaga cagtcgccgc ttcgtcgtcg tcctcttcgg gggagacggg cggaggggag
     4501 gaaagtccgg gctccatagg gcagggtgcc aggtaacgcc tgggggggaa acccacgacc
     4561 agtgcaacag agagcaaacc gccgatggcc cgcgtaagcg ggatcaggta agggtgaaag
     4621 ggtgcggtaa gagcgcaccg cgcggctggt aacagtccgt ggcacggtaa actccacccg
     4681 gagcaaggcc aaataggggt tcataaggta cggcccgtac tgaacccggg taggctgctt
     4741 gagccagtga gcgattgctg gcctagatga atgactgtcc acgacagaac ccggcttatc
     4801 ggtcagtttc acctgattta cgtaaaaacc cgcttcggcg ggtttttgct tttggtgccc
     4861 agggtaagat gaatatctac ggtattaata atagactttg taagaagtta ttaagtctat
     4921 attg
//
LOCUS       DAZUCC010000279         4900 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZUCC010000279 DAZUCC010000000
VERSION     DAZUCC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.281"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(127..750)
                     /locus_tag="ACHY09_004659"
     CDS             complement(127..750)
                     /locus_tag="ACHY09_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3887519.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRESGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            899..1384
                     /locus_tag="ACHY09_004660"
     CDS             899..1384
                     /locus_tag="ACHY09_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFS3887520.1"
                     /translation="MIKYIFALTLLLSGSVCADQIQGLVIRVLDGDTLEILQARHPVR
                     IRLVNIDAPEKKQPFGRWSMNQLKSLVVNTPVTVTYTQTDRYGRILGRVYTEDGMEVN
                     RWLVQHGAAWVYPDFNTDYTLPAYQREARTMKRGLWADKKPVPPWLWRKENKNSHNNY
                     K"
     gene            2028..2392
                     /locus_tag="ACHY09_004661"
                     /pseudo
     CDS             2028..2392
                     /locus_tag="ACHY09_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2402..2593
                     /locus_tag="ACHY09_004662"
     CDS             2402..2593
                     /locus_tag="ACHY09_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887521.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPGGE"
     gene            2627..2950
                     /locus_tag="ACHY09_004663"
     CDS             2627..2950
                     /locus_tag="ACHY09_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFS3887522.1"
                     /translation="MAYHSPDITLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMREQMSGIIRKTGTDEILFRLSKHHHLYEKK
                     KTKEG"
     gene            complement(3093..3188)
                     /gene="dqlB"
                     /locus_tag="ACHY09_004664"
     CDS             complement(3093..3188)
                     /gene="dqlB"
                     /locus_tag="ACHY09_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFS3887523.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELID"
     gene            3687..>4900
                     /locus_tag="ACHY09_004665"
     CDS             3687..>4900
                     /locus_tag="ACHY09_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFS3887524.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFAFLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMT
                     LGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTG"
BASE COUNT         1287 a         1346 c         1124 g         1143 t
ORIGIN      
        1 tttctgcgct ccggttaata cttctgattt atctcgcata aaatatcctg tctgtaatct
       61 gtcccggtaa acaatggtaa atttcagaat cagtgcgaat acggtaacaa agattcctcc
      121 tgtgctttaa agtgtgtagg cagaaaccag tctggtggtt ccggtcaggg agtctgatac
      181 ctcaacttcc cgggtgacta taaccatacc tgactgacgc agtctctgca catcagctgc
      241 aattcgctga gttcccagcc agaacaggcc atccagtgcc gttaccagca acccgctttc
      301 cagcgccagt ttcagccgtg ccgtcggggc tttaacctgc ccggcgatat cagtaaaaca
      361 acgcgtcgtc atgccatagc agtccatccg ccgtattcgc gtcgcaagac tgtagctgaa
      421 acaatccggc acgccggata agtcattctg cagactgtat acacatcccc gctgccgccc
      481 gttgaacagg gcttttttct gactgagcag ccattcccga cgcagagtgt caatcatctg
      541 cggtgtccgg gttaacagca gacgaaacgg cagctcaaaa ctggtcacat aatcaacatt
      601 cagtaatgct gtccgcagtc gctccctggc acctgccccg ctctcccggc attcacgcat
      661 gacatcagcc cactgacgca tcagtcgtgg agactcattc ctggccctga actgatattt
      721 ctcaatatga taatcaatac gttcctgcat atcgccaccg gatatctgtt gtttacccag
      781 acacagtgat tagcctacag gaataaatat cctccgtgaa gccaggtgcc tcagaagata
      841 aaaacatgta cttttttcaa cgtgtaatat taacaccacc tccacaggaa aatataaaat
      901 gataaaatat atattcgcac tcacacttct gctttccggg tctgtctgtg ccgatcaaat
      961 tcagggactc gttattcgtg tgctggacgg agatactctg gagatattac aggcccggca
     1021 tcctgtgagg attcgtcttg ttaatattga tgcaccggaa aagaaacagc cttttgggcg
     1081 ctggtcaatg aatcaactta aaagccttgt ggtaaatacg cccgtaaccg tgacatacac
     1141 acagacagac cgctacgggc gtattctggg acgggtatat accgaagacg gcatggaagt
     1201 taaccgctgg ctggtacagc atggtgcagc ctgggtatat cctgatttca acacagatta
     1261 caccctgccg gcataccaga gagaagccag aacaatgaaa cggggtctgt gggcagacaa
     1321 aaaacctgtt ccaccatggc tgtggcgaaa agaaaataaa aatagccaca ataattataa
     1381 ataagacatc tcacagaaat atatttcctg ccgttttatt tcccctgcca gtccccaacg
     1441 ggaaataacc cacagaaatt acacagatgt gaataataaa cctgttcgat gtatccgtca
     1501 caaaagagtt gatgtttgac tcagaagaaa atacgcctga agttaaggaa ggcttcagct
     1561 tcatgattgt tctgacatga acatatatcc ctctgtagcg ccctgccttt cataacattt
     1621 ctattacagc aacaacgaaa gacaacagca atgtcggcct tttacgtcca catgaatatt
     1681 ccacttatcc ggaattgtga cacagcccct gaatacagga ttgattgcca gtgcccacaa
     1741 atggcacatt ttcagtaaga ttctgaatgt tacattcccc cgtcatgtgt ggcacaactg
     1801 tcaccgggaa agaaacggaa agcacatgaa aaacatcaaa actcacaccg gactccttat
     1861 ccacaaagaa caaacgcggc gcgttcgtct tcatgaaact ccggccgcct ggtgccacac
     1921 gcaccgggaa tgctacagca aaacgaccgg tcgtcgctgc ggcagccctg agagtctcag
     1981 caggctgatt ctgagcagca ttcagcccat agaatgagat gacccgtatg aatgatggca
     2041 gacagacgcc tccgtcccct gaggcatgga aaatccggtt tctggcgaac ggtacacccg
     2101 gcacatggtt ttacacggat gagcagccgt cacaaaagag tggctgcggg cacattgtga
     2161 cgatgtggaa gtgatccccc tgtgccgtat gcatgacggt cccgttttcc gggaacacca
     2221 tattaatgag ctcagcgccg cactggccag aatgctccat atgcaccaca tactgctgga
     2281 acgaacccgg tacagagaaa atatccatga tgcagaatgt attcgtgaaa ttcacgaagc
     2341 tcccctgcag gctatcggca tcctggaaaa atacaaacgc acaaaatcat aaggcagttc
     2401 tatgtggata ctgattttct ggctctcatc cccctttaat gtctcctccg gcgatgcccg
     2461 gcaacccaca ctggccgttc acagtcagga gttccgtact gaaaaagcct gtcgggatgc
     2521 cttcaccgcg ctgaaaaaag aaaacgtggg agaattcgct ctccggggcg tctgcgtccc
     2581 cggcggagaa taaaccggag atgatgatta gccacaggag tattcaatgg cataccattc
     2641 cccggatatc acactgcatt atccgtatct gcgccggatg tcctgggcgc agataacggc
     2701 agcagctgaa gatgcggaaa tacatcatga ttatgcccgg gcattaatat tgtggcagca
     2761 tgcatatcat gctgccacgc tgaccctcaa caaaaacctg gcagttgcta aaatcgactt
     2821 ctgtattaaa cgtatccgga tgcgtgaaca aatgagcggg ataatccgga agactggcac
     2881 agacgaaata ctcttccgtc tgagtaagca ccatcattta tatgagaaaa agaaaacaaa
     2941 agagggatga caatcccatg atatgggaaa aacttgccat gcttactgca ggtgcatttt
     3001 tgatactggc agtcctgtgg gggtaataat atcctgttta ttaaagggca gaaggcggtg
     3061 cccctctgcc cttaagtacg tttccgggat tatcagtcaa taagttcccg gatcagttcc
     3121 aggattgcta tcactgcgtt agccacagca acaaccgccc tgagaacgat gattaacagg
     3181 ttaatcatcc gtttctccgt caatggagga aatcactgtt gtttactctt acattgcctg
     3241 tcacaacagc tctttcattg ttagatgtaa agtgatttct ctccagccag agcactgaac
     3301 acagcaccac ctgttcgatc agccaggact taaaatctcg cgttcatcgt cccattggag
     3361 atcacggcag aagcatcacc agacagagct aacggagcga ttatagtcat ttttctgatt
     3421 gcaaaaaaga gaagcgcagc ctactatgat ggtgttagat gtagagtgat ttccctttcg
     3481 gtcagagtca ccaactccgc cccggaatag caaaaaaacc gacctcacca gtcggttttt
     3541 ttgtgcctgt ctctcactgc cttctgctgt tcctgtgaaa ataccctggt tatttcatcc
     3601 ctgcccggtc attaccggcg attgttcgca ttaattccac tcagcctcat cccgaaattt
     3661 atccatttat cgatggagga atactcgtgt cttaccaaca ctcacccaca actgaagacc
     3721 cgatgaatat atggtatatc gttggcacca tatgcctgct ctttgcgttt ctcatctggc
     3781 tctttttacc cgacatcgtc tttgccacct gcctgatcct gcacacaatg tgggggatga
     3841 ctgactgggg gccttttcac cattttgccg ccccccgtta taacctgctg gccaacaccg
     3901 ccaacaacgc tgcaacgatc actttcagcc agtggctgga tgtcatgtca cagaccgtgg
     3961 ggatcctctg gctgattctg cttcccatga cgctcggctt tctgtggatg tggtttcagc
     4021 atccggccca gccccgcttc acccgcagac cactgaatat ccacacgctg ccacacctgt
     4081 tttcatcact gtcaccggcg atcgcccccg ttctggctga cggagacagc aacagactct
     4141 tccacggaca aaaacgtccc gagcgccgtg tcgcactaac ccccgaagtc tttgtggagc
     4201 agaacaacct tatccgcaac atgcagctcg atgttgcgtc cacacgccag tgcttcatgg
     4261 cgcaactggg gcaaccactg accagctgga aagacatggc cccacatgaa aaagcgctct
     4321 ttgccatctt tggcctgcag ttcttcctcg gtgaccggaa agcagcggta gccctgatga
     4381 acaacctcaa tctctcctgc aggctcaaaa gtaaacggga ccaggggcgt ttcagcacgc
     4441 cggtgtattc actggccaga aacgccttta tccgggtcat caaaacagaa ggcgcacaac
     4501 agtggctcag gcagcaccgt tacgttcgca gcggtctggt ctggctttac gcacatgatt
     4561 tgcgcctgac gcctccgaac tggctgtggc tcaagggcgt tgaccggacg ctcttctatg
     4621 cccttcaccg ggcaaacacc acaaaaggct tcatcgaagg ggccggcgtg gtggccgttg
     4681 cccgggcaga aaatgaagcc agccgtctcg gcctcccctg cccggaaccc tgtgtggaag
     4741 aggccattga agggcttcga cgggacatgc tggggctggg cctgatatgg gacgaacctc
     4801 agccagatcg cgatcgtaaa cgtcagatca ggacccgctg gtcactcacg gatgacgtga
     4861 taccccgccg ccatgataac gacgaagaca ctgacactgg
//
LOCUS       DAZUCC010000280         4800 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZUCC010000280 DAZUCC010000000
VERSION     DAZUCC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.282"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(173..934)
                     /gene="kduD"
                     /locus_tag="ACHY09_004666"
     CDS             complement(173..934)
                     /gene="kduD"
                     /locus_tag="ACHY09_004666"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFS3887525.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(964..1800)
                     /gene="kduI"
                     /locus_tag="ACHY09_004667"
     CDS             complement(964..1800)
                     /gene="kduI"
                     /locus_tag="ACHY09_004667"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS3887526.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGIINIGGAGTITVDGQCYEIGHRE
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2087..3268)
                     /gene="yqeF"
                     /locus_tag="ACHY09_004668"
     CDS             complement(2087..3268)
                     /gene="yqeF"
                     /locus_tag="ACHY09_004668"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFS3887527.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3523..4752
                     /gene="yqeG"
                     /locus_tag="ACHY09_004669"
     CDS             3523..4752
                     /gene="yqeG"
                     /locus_tag="ACHY09_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3887528.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1259 a         1205 c         1063 g         1273 t
ORIGIN      
        1 ttgaattatt gagattatta ttaaccacct aattttaaag cagataaaat tcataaagtt
       61 cattaattga taattgatat ggattatttc ataaccatga tatggattat gatgatctac
      121 aggtataaaa aaccctgcca tgcggcaggg tcataaaagt aagagaaatg aattaacgag
      181 ccagccaacc gccatccacg gcaatggtat aaccattcac ataatctgaa gcgctggagg
      241 caaggaacac taccggcccc atcaggtcac tcggcagtcc ccaacggcca gctggaatgc
      301 ggtcgagaat ttccgcgcta cgttgttcat ctgcccgtag ttgttgagta ttgttggttg
      361 ccatgtaacc cggagctatc gcattaacat taatgttgtg tttagcccat tcattcgcca
      421 tcaatcgcgt cacgcccatc acgccgcttt ttgatgcggt ataagaaggc acacggatcc
      481 cgccctggaa ggagagcatt gacgcgatat tgataatctt gccgccattg ccttgcgcga
      541 taaagtgttt cgccgctgcc tgagacatga agaatacgct cttgatattc aggttcatga
      601 catcgtccca gtctttttcg ctgaactcga gagcgtcttc gcggcgaatc aatccggcgt
      661 tattcaccag gatatcaata tgaccaaact cagctaccgc gcgatccagc agtgctggaa
      721 tgccatcaat ctttcgcaga tcggcggtca ggcttaaaaa acgacgcccc agcgccgtga
      781 cctgcttgat ggtttcagtc ggttcaacga tgttaatgcc aacaatgtca cagcctgctt
      841 gcgccagccc caacgccatc ccctggccca gcccagtatc acaaccagtg acgaccgcaa
      901 ctttaccttc gagagaaaat gcacttaaaa tcataacaat accttattcg tttatgccca
      961 caactagcgc aaatctttaa cggccacatg gtccatatca tcaaaaacct ggttttcacc
     1021 gaccatgccc cagataaagg tataagcttt ggttccgacg ccggaatgga tcgaccagct
     1081 cggggagatc accgcctgct cgttatgcat cacaatgtga cgcgtttctt gcggttgccc
     1141 catcatgtgg aaaacgcagg cgtcatcatc catattgaaa tagaaataaa cttccatccg
     1201 gcgctcgtgg gtgtgacacg gcatggtgtt ccacaagtta cctggagcca gctccgtcag
     1261 ccccatactc aattggcagg tttccagcac atccgggaca aaatatttgt taatcgtgcg
     1321 acggttactg gtgaggttat cgcctaacgt gactggagat acttcgtccg gtgtgacttt
     1381 tttggtggga tacgtcgtat gcgcgggtgc gcaattgtaa taaaacttcg ccggagtggc
     1441 ggtatcaata ctggcaaaga caacttcttt tgcaccttta ccaacataca gggcttcgcg
     1501 gtgaccgatt tcatagcatt gaccatcgac agtaatcgta ccggcaccgc caatattgat
     1561 aatacctaac tcgcgacgtt caaggaaata gcttacgccg agttgtttac caacttcccc
     1621 gccaacggaa accgttttag ttaccggcat aatgccgcca acaataatac ggtcaatgtg
     1681 gctgtaaacc atggtgtact catcggcgac aaataccttt tcaaccaaaa attcattgcg
     1741 cagcccctgg gtatccagcg tttttgcgtg cgcactgtgg atgctctgtc ttacgtccac
     1801 atcaacctcc gataatcagt gaagcgtgaa aatagaaaac gatagtgaaa cgctgtttcg
     1861 ttttctctat aaggggatga tatgcccacg ggatagggtt ttcaattaaa aataaaacaa
     1921 tgtttcattt ttaaggtaag gactgaaaaa agtgcagtgt cgatcacgaa tatgtcatga
     1981 gaatggaaaa atcagggagc cctgaaaagg tatgtcgact cgccagccac cattattcaa
     2041 cagattaatg acggctgata tttatcggta aggaggaaat gcagagctat tcgtcacgtt
     2101 caatggtcaa tgccacgccc tggcccccgc cgatacaaag cgtagccagt cctttgcggg
     2161 cattacgttt caccatttca tgaaccagag aaacgaggat tcggcaaccg gaagcgccta
     2221 ttgggtgacc gagtgcgatc gcgccaccat tgacattgac acgacgctca tcccattcaa
     2281 gcatcttgcc aaccgaaagc gcctgtgcag caaacgcttc attagcctcg ataagatcga
     2341 catcagccaa ctgccagcct acacgctcca ggcaacggcg ggtcgcatac accggcgcaa
     2401 ttcccatcaa tgccggatct acaccaacgc tggcaaatgc gcggatgcgg gccagcacgg
     2461 gtaaattcaa cgctcgtgct ttggcttcgc tcatcatcat tactgcagct gcgccatcgt
     2521 ttatggatga tgcattaccc gctgtcacag aaccgagact atcaaatgaa ggatttaaac
     2581 gggctaagcc ttctgcactg gcgtcagtgc gtggctgctc atcggtatca acaaccaacg
     2641 tctgaccgtt actttgggtc attaccggga cgatctcatc tttaaatcgt ccagcgtcaa
     2701 tcgccgctcg cgctttttgt tgcgagctaa gtgcgtaagc atcctgcaac tgacggctga
     2761 tgccatattc gcgagccaga ttttcggcgg tgacaccaat atgataatca ttgaaggcat
     2821 cccacaaccc atcgtgcaca agactatcaa ccaactggct attgccaagc tgtgcaccgg
     2881 tgcggctatc agtcagaaca tgtggtgcgc ggctcatgtt ttcctggcca ccggcgatga
     2941 caatatcagc ctcgccacac tgtatcgcct gagtagccag atgcagtgct ttaagcccgg
     3001 aaccgcaaac gtcattaata gtgattgcag aaacgctatt aggaagacca cctttaatag
     3061 ccgattgcct tgccggattc tgccctgccc ctgcagtcaa cacctgacca agaattactt
     3121 catccaccgc atatgcagga acgccggtac gttctattaa cgctttcacg accaggctgc
     3181 caagttccac ggcggaatga cccgctaacg caccacggaa gcagccgatg ggtgtccgta
     3241 acgccccgac aatcacaacg tctttcatca caacctcgca ccaattaaca gtgcaatagt
     3301 aaatgaatgt taattacagt tatcttaatt gtttaaaaaa agtgattttt atcacaaagg
     3361 aaatatgcct gagtagcaat cagagacata actggcacgt aaggtttgca accactaacc
     3421 caccaataga gaggtagaaa gggcgttaat ctcccatact taacctggtt tatggtaaat
     3481 tgccctccat tttgtttaat ttgtagatga tacgttcaga taatgtctaa tatttggtca
     3541 aaagaagaaa ctctgtggag tttcgcgctc tacggcacag ccgttggtgc aggcacgctc
     3601 ttccttccta ttcagttagg ttcggcgggg gctgtggtcc tgtttattac tgctctggtc
     3661 gcctggcctt taacatattg gccacataaa gccttatgcc agttcatcct ctcatcgaaa
     3721 acatcagcag gtgaagggat aacgggcgcg gtaacacact actatggcaa gaagattggt
     3781 aatctgatta ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat atatgcagtg
     3841 gcaattacca actcacttac agaacagctg gcaaagcata tggttattga tcttcgcatc
     3901 cgtatgttgg tgagtctggg ggttgtatta atcctgaatc tcatttttct gatgggacgc
     3961 catgccacta ttcgggtaat gggatttttg gtattcccat tgattgccta tttcttattt
     4021 ctttctattt acctggtcgg tagttggcaa cctgatctat taacaaccca ggtagagttc
     4081 aatcagaata cccttcacca gatatggata tcgattcccg tgatggtttt cgcttttagc
     4141 catacgccca ttatttctac gtttgccata gacagacgtg aaaaatatgg cgaacacgct
     4201 atggataaat gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat aagtgtattg
     4261 ttctttgtct ttagctgcct gctttctatt ccaccttcgt atattgaagc ggctaaagaa
     4321 gaaggggtta ccattttatc ggcgctttct atgctgccga acgccccagc atggttgtca
     4381 atttccggga ttattgtcgc agtagttgcg atgtcgaaat cattcctggg tacgtacttt
     4441 ggcgttattg aaggtgccac agaggtcgtc aaaacaacat tacagcaggt tggtgtaaag
     4501 aaaagtcgtg catttaaccg cgcactatca attatgttgg tatcgctgat taccttcatt
     4561 gtttgttgca ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg cccgctcatt
     4621 gccatgatac ttttcatcat gcctacgctg tcaacgtatc tcatcccggc gcttaaaccc
     4681 tggcgttcca tcggaaatct gattaccctg atcgtgggta tcctgtgcgt atcggtaatg
     4741 ttctttagct aatatccgtt gtggatggtt caaaattgct tcaattttga accatcccag
//
LOCUS       DAZUCC010000281         4799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZUCC010000281 DAZUCC010000000
VERSION     DAZUCC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.283"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..956)
                     /locus_tag="ACHY09_004670"
     CDS             complement(<1..956)
                     /locus_tag="ACHY09_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS3887529.1"
                     /translation="MLKDGTGKAVPGCTIELKARRTTETVIVTTVAYGQPGETGSYSM
                     DVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAV
                     AAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGE
                     TGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAATPKAVKSA
                     MDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEFVAWLKEKGA
                     FDQPYWMMKASLLASMNKVITDVGPGKLNLGGCVIEVMGTYEA"
     gene            complement(1120..1260)
                     /locus_tag="ACHY09_004671"
     CDS             complement(1120..1260)
                     /locus_tag="ACHY09_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3887530.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHTSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            complement(1459..>4799)
                     /locus_tag="ACHY09_004672"
     CDS             complement(1459..>4799)
                     /locus_tag="ACHY09_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3887531.1"
                     /translation="SILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAE
                     TALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVETSSKGDRNPSSVRLLIQLERNG
                     NWVTEKDITINGKTTSQYLTSVILNNLPERPFNIRMVRETADSTTDQLQNRTLWSSYT
                     EIIDVKQCYPNTAIVGLQVDAEQFGGQQLTVNYHIRGRIIQVPSNYDPEKRTYSGIWD
                     GSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGT
                     EPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVV
                     VDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMDA
                     FGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGG
                     RILSIDAASRTLTLDREVTLPEAGTSTVNLINGSGKPVRVDITAHPAPDRIQVSVLPD
                     SVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPLS
                     GSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGNGENSRLVT
                     SAITADTEHRFRGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGYY
                     QITAVPHLTVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDF
                     WFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGMWELIDNSQLDDE
                     MAEMKSTITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNG
                     IPYVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLK
                     RLFAVSITSSGNPPTFSLTPEGRLTARNADISGHISANSGTLNNVVIAENCTINGTLK
                     AENIIGDLVKCAGVAFPVDGSYLANGTRTLTVYDDHSFDRQIIIPPIIYVGSKQESRT
                     SNDIWTECFLHVDQNGRRIYSGRSVTEPGIFSGIIDMPAGGGHITLSFTVSSRRQNGS
                     FGSSRISNLQAIVVKKNSAGISIR"
BASE COUNT          878 a         1547 c         1211 g         1163 t
ORIGIN      
        1 gcctcatacg ttcccatcac ctcaatgaca cagccaccca gattgagttt tcccggtccg
       61 acatccgtga tgactttatt cattgacgca agcagtgatg ccttcatcat ccagtaaggc
      121 tggtcaaatg cccctttttc tttcagccag gcgacaaact cactcgtggt ccattcaccg
      181 gcccccgtat gaatgtcccg cccgtatact ctggctgccc ccacggtgtt cagaaacttc
      241 accttatccg ggatatcgtc accgtttttc gctttctcca ggcatccgtc cgctttgtcc
      301 atcgccgatt tcacagcctt cggcgtggct gccttcgttt catcatcact gtccgttgcg
      361 ctgcttaact gcacaattcc cttctgtgcc gtcgtcgcat ccggtcctcc cggctcgcct
      421 ttttcgccct tctctccccg ttcacctttc acaccctgaa gcccctgtgg tcctgtctca
      481 ccacgctcac cctttggccc ccgctcgccg gtatcgcctt ttggcccggg aataccctgc
      541 ggcccggtgt cccccttatc ccccttcggt ccccgcgcat tctctgcccg tttttttgcc
      601 tcctccgcac tggctgcgga cgcttccgca cgtttcagga tttccgctgc caccgcttcc
      661 agctctgcaa gggctttcgg gtaatactgt gcgtcttcca ggtccatcag aaatttattc
      721 agcgttcccg gtgcagaatc cgccttcacc agaatgtcac ccacatatga cggcgcgtac
      781 ccttccgtgt tcagcgtcac ccggtacaac cccggctcaa catccatact gtaactgccg
      841 gtttcccccg gctgaccata cgccaccgtg gtgacaatca ccgtctccgt tgtgcggcgc
      901 gctttcagct ctatcgtgca tcccggtacc gcttttcccg taccatcctt cagcacaccc
      961 gatattctga ctgtcatgga tttcccccat aaaaaaaccg caggaccggt ttcccggcct
     1021 gcggtaatat ttgtggtttg ttggtgttaa aacggtgcca tccggctgac caccctcagc
     1081 aaccggtcgg cgggggatat tctcccccgc cacggtttct tactgcttac actgtaagaa
     1141 cgccgcaatc tccgcgcccg ctatccggaa ccggaactcg cacagtgaag tgtgggtgat
     1201 ccagataatg agcactaccg tgatacaaat cacggtggtt tttaacggtt tttgcgacat
     1261 aaacgcttgc tccttttacg gagaggcgct aaccttcaat ttgctgaaga tcgaaagtca
     1321 ggcctcgggt taaacatgaa tgtttgtccg gggccttcgt ctttccggcc ttcaggtgtt
     1381 ccctccggcc atcagccaaa ggcacccgcg catactgtac ggtttttgtc tccttccggc
     1441 aatcccgggg cgcgatgttc agcggatact gatccccgcg ctgtttttct tcaccactat
     1501 cgcctgaagg ttactgatac gtgaactgcc aaaactcccg ttctgacgtc gtgaacttac
     1561 ggtaaaactc agggtgatat gaccacctcc ggctggcata tcgatgatcc cgctgaaaat
     1621 tcccggctct gtcactgacc tgcctgaata aatccggcgt ccgttctgat caacatgcag
     1681 gaagcactct gtccagatgt cattactggt acgggattcc tgttttgacc cgacatagat
     1741 tatcggcggg attataatct gccggtcaaa gctgtgatcg tcatacaccg tcagcgttcg
     1801 tgtaccgttc gcaaggtaac taccatccac cggaaaagcc acccctgcac atttcacaag
     1861 atcaccaata atgttctccg ctttcagcgt gccatttatc gtacagttct ccgctatcac
     1921 gacattattg agcgtgcccg agttcgcact gatatgtccg ctgatgtccg cattgcgggc
     1981 cgtcagcctg ccctccggcg tcagggagaa cgtcggggga ttgccggacg aggtgatgct
     2041 cactgcaaac aaccgcttca ggaacacgtc gttcatgaac agctgattcc cctgcgccac
     2101 aaacagcggc gtggtgttgc cgttctccgg gttaatcatc gcgatacggt ccgcctgcag
     2161 cagtatattg ctcaggggct ggccatcagc atcctcaatc cccgcaccta taccggcaac
     2221 atacggaatg ccgttttttg ttttctgcac cttcagcatg tacagcgcag ccaggtcgtt
     2281 atttgtgtct gtctgcaccc gctgtatctg ctgtatggtg gcgctctggt cttccagggt
     2341 tttactgacc gtctgcgtga tttcattgcg ggtttctgtg atggtgctct tcatctccgc
     2401 catctcatca tcaagctggc tgttatcaat cagctcccac atcccctgtg ccagatgcag
     2461 ttttcctatc ttttcccgaa acagccccag atacccttcc gcatcattgc tggcccggcc
     2521 actggcttcc acaaacgcag atttccccac caggttgacg ctgcgcacat aaaaccagaa
     2581 atccttcccc ggcttaatgt gcgggccgga gacactccac tggctgcctg tccccagata
     2641 acgggcagag gtttccacct gagatgtgtc tgcgattttt gcctccgaaa accagaactc
     2701 aaattgtacc gtcgggtcat acaccgtaag atgcgggacc gccgttatct gataataccc
     2761 cggcgtcagc tcaatgctgg ccggtgctgc aggcgcatta atccggaatg tggtggtggc
     2821 aggttcgccc tgctggccgt agctgtttat cgccctgacc gtcagggtgt attccccggg
     2881 cggcaggcct ctgaaacggt gctccgtgtc ggcagtgatg gcgctggtca ccaggcggct
     2941 gttttcaccg tttccgctgg tcaggcgcag actgaagcgc acgcccttca ccacccgcgg
     3001 cgtgtcccat ttcgccagcg ccagatactg gccgtctgag gcactcacct ccaccgtgag
     3061 gtgctgcact gccggcggga tgacgctgtt cagtgaaccg gagagcggct caaagcgggc
     3121 cccgttatcc acgatggctt ctttttccgg tacgtgctgc actgccgtga tggcaaaggt
     3181 gccgtccgtg ttttcccgga tggaaacaca gcggaacagg cgacgacgca gtgacggcag
     3241 ggagagtccc cacacaccgt atgtctccac gctatcaggc aggacgctga cctgtatccg
     3301 gtccggggcg gggtgtgcag tgatgtccac gcgcaccggc ttaccgctgc cgttaatcag
     3361 gttcaccgtc gatgtccctg cttccggcag tgtcacctca cggtccagcg tcagggtacg
     3421 gctggcggca tcgatggaca ggatacgtcc gccggtcagg gtcccggcgt agtcgttatc
     3481 acagatttca atgatgtcac cgggcgtgtg ccgcagcccc tgtgacccga gcgtgaaatc
     3541 caccgtctgc gtttccagca gttctgtctt tatcacccac agtccggcac ggtgggcctg
     3601 accgcggctg gtacagccaa acgcatccat cttcagcaga ttgcgcccgt agcgcaggat
     3661 ggcgtccggg tcttccacca gttccgtgga agtctgccag ccgttctgcg ggtcggtgta
     3721 attcacctcc accgccgtgt gccggtcctt cagggcactg aagctgtagc ggaaccccac
     3781 gccgttatca tccaccacca catcgctgtt ggtgtagggc cataccacat ccgacggacg
     3841 gtcctgaacg aacgtcagcg tctgaccgtt ccataccggc atacagcgca tcgccgagca
     3901 gaaatccccc aggacatccc agaccttacg ctgctgtgac aggtacgcat tgaaagtcat
     3961 ccgcggctct gttcccccga aaccatccgg gaccgtctgg tcacagtact gcccgatggc
     4021 atacagtgcc cacttgtcca cgtccgcggc ccccaggcgt tttcccatcc cgtagcgcgg
     4081 gtgggtcagc atgtcccaca gacaccaggc cgggttattg ctgtatgccg gtttcagact
     4141 cccgtcccag ataccgctgt aggtgcgttt ttccgggtca tagtttgacg gcacctgaat
     4201 gatgcgacca cggatatggt agttcaccgt cagctgctgg ccaccaaact gctccgcatc
     4261 cacctgcagc cccacaatgg ccgtgttcgg gtagcactgt ttcacatcga tgatttcggt
     4321 gtatgacgac cacagcgttc tgttctgcag ctggtccgtg gtgctgtccg ccgtctccct
     4381 gaccatccgg atgttaaagg ggcgctcagg gagattattc agaatcaccg acgtcaggta
     4441 ctgcgaggtg gtcttgccgt taatggtgat atccttctcc gtcacccagt taccgttacg
     4501 ctcaagctga atcagcaggc ggacagaaga gggattacgg tcaccctttg agctggtctc
     4561 caccagtgac tgcaccccga aggtgacccg cagacggtca atgttcgctg acgtgatggt
     4621 gcgcgtcacc ggctttgcct tcgtcacctc cacgcccagt gcggtttccg ccccggagga
     4681 ctcaaagcct tccggcggtg tctgctcctg ctccccggcg cgccagaccg cggtcacacc
     4741 gtgtatcacg ggattaccgt ccgtgtccgt cagcggggtt ttgttcacca gaatactct
//
LOCUS       DAZUCC010000282         4781 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZUCC010000282 DAZUCC010000000
VERSION     DAZUCC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.284"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            233..1195
                     /gene="tauA"
                     /locus_tag="ACHY09_004673"
     CDS             233..1195
                     /gene="tauA"
                     /locus_tag="ACHY09_004673"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3887532.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1208..1975
                     /gene="tauB"
                     /locus_tag="ACHY09_004674"
     CDS             1208..1975
                     /gene="tauB"
                     /locus_tag="ACHY09_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFS3887533.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVVDNVVFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1972..2799
                     /gene="tauC"
                     /locus_tag="ACHY09_004675"
     CDS             1972..2799
                     /gene="tauC"
                     /locus_tag="ACHY09_004675"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFS3887534.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTFSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2796..3647
                     /gene="tauD"
                     /locus_tag="ACHY09_004676"
     CDS             2796..3647
                     /gene="tauD"
                     /locus_tag="ACHY09_004676"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFS3887535.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWCWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3687..4661)
                     /gene="hemB"
                     /locus_tag="ACHY09_004677"
     CDS             complement(3687..4661)
                     /gene="hemB"
                     /locus_tag="ACHY09_004677"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFS3887536.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1073 a         1226 c         1384 g         1098 t
ORIGIN      
        1 tgtattgctg ttaataaaat aaaagacatt ataatctgct tgtttatgca ttaataaaat
       61 gaagtgcagt aacttactga attgtcagag tgggtgtaat tgcatattta atctttcctt
      121 tgccactttt tcgctaagaa taaaatcatc tgtgcgataa caactaattc ttttaatgaa
      181 tgtttttatt cctgaatact gctcccataa caagacaggg gagcagacaa tcatggcaat
      241 ttcatcgcgt aacacacttc ttgccgcact ggcattcatc gcttttcagg cacaggcggt
      301 gaacgtcact gtggcgtatc aaacttcagc cgaaccggcg aaagtggctc aggccgacaa
      361 cacctttgct aaagagagcg gggcgagcgt tgactggcgt aagtttgaca gcggagccag
      421 catcgtgcgg gcgctggctt ctggcgacgt gcaaatcggt aacctcggtt ccagcccatt
      481 agcggtggca gccagccaac aggtgccgat tgaagtcttc ttactggcgt caaaactggg
      541 taactccgaa gcgctggtgg tgaagaaaac tatcagcaaa ccggaagatc tgattggcaa
      601 gcgcatcgcc gtaccgttta tctccaccac ccactacagc ctgctggcgg cgctgaaaca
      661 ctggggtatt aaacctgggc aagtggagat tgtgaacctg cagccgcccg caattatcgc
      721 cgcctggcag cggggagata ttgatggtgc ttatgtctgg gcaccggcgg ttaacgcgct
      781 ggaaaaagac ggcaaggtgc tgaccgattc tgaacaggtc gggcagtggg gcgcgccgac
      841 gctggacgtc tgggtcgtgc ggaaagattt tgccgagaaa catcctgagg tcgtgaaagc
      901 gttcgctaaa agcgccatcg atgctcagca accgtacatt gctaacccag acgcgtggct
      961 gaaacagccg gaaaacatca gcaaactggc gcggttaagc ggcgtgcctg aaggtgacgt
     1021 tccggggctg gtgaagggga atacctatct gacgccgcag cagcaaacgg cagaactgac
     1081 cggaccggtg aacaaagcga tcatcgacac cgcgcagttt ttgaaagagc agggcaaagt
     1141 ccccgccgtg gcgaatgatt acagccagta cgttacctcg cgcttcgtgc aataaaagga
     1201 ggcgcagatg ctgcaaattt ctcatcttta cgccgattat ggcggcaaac cggcactgga
     1261 agatatcaac ctgacgctgg aaagcggcga gcttctggta gttctggggc cgtctggctg
     1321 cggcaaaacc accttgctga atctgattgc cggttttgtg ccttatcagc atggcagcat
     1381 tcaactggcg ggtaagtgca ttgagggacc gggagcagag cggggcgtgg tttttcagaa
     1441 tgaagggctg ttgccgtggc gtaatgtggt agacaacgtg gtgttcggcc tgcaactggc
     1501 aggtgtagag aaaatgcagc gactggaaat cgcacaccag atggtgaaaa aagtggggct
     1561 ggaaggcgca gaaaaacgct acatctggca gctttccggt ggtcaacgtc agcgggtggg
     1621 gattgcccgc gcgttggcgg caaatcccca gctgttatta ctcgacgaac cgtttggtgc
     1681 gctggacgcc ttcacccgcg accagatgca aaccctgctg ctgaaactct ggcaggagac
     1741 gggaaagcag gtgctgttga ttacccacga tatagaagaa gcggtgttta tggcgactga
     1801 actggttctg ctttcacccg gcccaggccg tgtgctggag cggctgccgc tcaactttgc
     1861 tcgccgcttt gttgcgggag agtcgagccg cagcatcaag tccgatccac aattcatcgc
     1921 catgcgcgaa tatgttttaa gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg
     1981 ctcattaatg aaaaactgca ttcgcatcgg ctgaaatggc gctggccgct ctcgcgtcag
     2041 gtgaccttca gcattggtac gttagcggtt ttgctcactg tatggtgggc agtcgcggcg
     2101 ctgcaactga ttagcccgct gtttttaccg ccaccgcaac aggtactggc aaaactgctc
     2161 accattgccg ggccgcaggg atttatggac gccacgctgt ggcagcatct ggcagccagc
     2221 ctgacgcgca ttttgctggc gctactcgcg gcggtgttga tcggtattcc ggtcgggatc
     2281 gccatggggc taagcccgac ggtgcgcggc attctggatc cgataatcga gctttatcgt
     2341 ccggtgccgc cgctggctta tttgccgctg atggtgatct ggtttggtat tggcgaaacc
     2401 tcgaagatct tactgatcta tttagcgatt tttgcgccgg tggcgatgtc ggcgctggcg
     2461 ggggtgaaaa gcgcgcagca ggttcgcatt cgtgccgccc agtcgctggg tgccagccgt
     2521 gcgcaggtgc tgtggtttgt cattttgccc ggcgcgctgc cggaaatcct caccggatta
     2581 cgcattggtc tgggtgtggg ctggtctacg ctggtggcgg cggagctgat tgccgcaacg
     2641 cgcggtttag gatttatggt tcagtcagcg ggtgaatttc tcgcgactga cgtggtgctg
     2701 gcggggatcg cggtgattgc gattatcgcc tttcttttag aactgggtct gcgcgcgtta
     2761 cagcgccgcc tgacgccctg gcatggagaa gtacaatgag tgaacgtctg agcattaccc
     2821 cgctggggcc gtatattggc gcacaaatta cgggggccga cttaacgcgc ccgttaagcg
     2881 ataatcagtt tgaacagctt taccatgcgg tgctgcgcca tcaggtggtg tttctgcgcg
     2941 atcaagctat tacgccgcag cagcaacgcg cgctggccca gcgttttggc gagctgcata
     3001 ttcaccctgt ttaccctcat gctgaagggg ttgacgagat catcgtcctg gatacccata
     3061 acgataatcc gccggataac gacaactggc acaccgatgt gacatttatt caaacgccac
     3121 ccgcaggggc gattctggcg gcgaaagagt taccctcgac cggcggcgat acgctctggg
     3181 ccagtggtat tgccgcatat gaggcgctct ctgttccctt ccgccagctg ttgagcgggt
     3241 tgcgtgcgga gcatgatttc cgtaaatcgt tcccggaata caagtaccgc aaaaccgagg
     3301 ctgagcatca acgctggcgt gaggcggtcg cgaaaaatcc accgttgcta catccggtgg
     3361 tgcgaacgca tccggtgacc ggtaaacagg cgctgtttgt gaatgaaggc tttaccacgc
     3421 gaattgttga tgtcagcgag aaagagagcg aagccttgct gggcttttta tttgcccaca
     3481 tcaccaaacc ggagtttcag gtgcgctggt gctggcaacc aaatgatatt gcgatttggg
     3541 ataaccgcgt aacccagcac tatgccaatg cggattacct gccacagcgg cggataatgc
     3601 atcgggcgac gattcttggg gataaaccgt tttatcgggc ggggtaaggg ggaaatgccg
     3661 ggttggataa cccggcataa tgatcattaa cgcagaatct tcttctcagc caaatccagc
     3721 gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa tcgaacctaa gctttcgagc
     3781 acgactttct cttcatctat agcacccgcc agcgcggcga acttaatcat cgcgtactca
     3841 ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc acgcacgatg
     3901 tcgaggtatg ctccggcagg tttaaccatc aggcagtctg cgccctgggc ttcatccagc
     3961 agtgactcac gaatcgcctc acgacggttc attgggttca tctgatagct tttgcggtcg
     4021 ccttttaatg cgcttccggc agcttcacgg aacgggccat aaaaggagga ggcgaacttg
     4081 gtcgaatacg acataatcgc cgtatcttta aagcccgcag cgtccagcgc ctggcgaatc
     4141 gcctgtacct gaccatccat cgcagcggaa ggggcgatga aatctgcgcc tgcagcagct
     4201 gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac gccatgctcg
     4261 cacagcacac cgcagtgacc gtgagaagtg tattcacaga aacaagtgtc tgacatgacg
     4321 atcatttctg gcacagtctg cttgcagatg cgcgacatac gcgccaccag tccatcttcc
     4381 cgccaggcgt cgctgccggt ttcatcggta tggtgagaga tgccgaaagt catcacggaa
     4441 cgaataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc tggaatgcgc
     4501 atcacgcctg gcatggcttc aacggctttg tagtcgtcaa tttcttcttc aacaaagatc
     4561 ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg cagcgcagga
     4621 gatttgcgca ggcgacgagg gcgttggatt aaatctgtca tggtctgcct gatgtttgtg
     4681 gaatcgagga gcatagtata cctgaagcag gctgggtgtg ttttacgaaa gttgtcgcga
     4741 tgttgacaag aagagaatga aaaagaggtc aggacatctt t
//
LOCUS       DAZUCC010000283         4768 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZUCC010000283 DAZUCC010000000
VERSION     DAZUCC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.285"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..559)
                     /locus_tag="ACHY09_004678"
     CDS             complement(<1..559)
                     /locus_tag="ACHY09_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3887537.1"
                     /translation="MNLIFSKYSVFFILFAFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGTYDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRLSAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANALLYFDIDY"
     gene            complement(559..3153)
                     /locus_tag="ACHY09_004679"
     CDS             complement(559..3153)
                     /locus_tag="ACHY09_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFS3887538.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDENQLIPCLSLAD
                     LLSLGINKNALPEQALALSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHSKYSTTVGQYRSNSYNQKSPYVFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKTLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVAWNYS
                     KSPGTNADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSG
                     SLLEQHNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYDNEGNQQINYGISG
                     ALVVHENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNN
                     ISLDPMSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATA
                     SAQIKNSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELNNPV
                     SYATLECR"
     gene            complement(3190..3942)
                     /locus_tag="ACHY09_004680"
     CDS             complement(3190..3942)
                     /locus_tag="ACHY09_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3887539.1"
                     /translation="MKCIIGNKKWVYGFRDELKVSQNIIRSGILFLLSILFINNASAG
                     GFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFML
                     PTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFY
                     RPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNTFVPPKSDI
                     TVSSAVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            complement(4043..4747)
                     /locus_tag="ACHY09_004681"
     CDS             complement(4043..4747)
                     /locus_tag="ACHY09_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3887540.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDAAQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDIEAQ
                     GASNQTDFNINLTECSNTEDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT         1390 a         1086 c          895 g         1397 t
ORIGIN      
        1 gataatcgat atcaaaatac aacagagcat ttgcttgccc ggcatgtaca ttttcctgag
       61 ttgatttata gcgaagttta aacgtcaaat tagcatcacc gttttcattt aacactaaat
      121 cacgaaaacc tgattcacca ttaatagcaa tcgccacatc attgttatcc aatatttcta
      181 ttcccagtcc agtcgcacta tctgcaccgg cggagagacg cagtaatgcg ctattaatgg
      241 catctgcatc accagtaaac cgcacattgg cgctcacacc agaactataa acagtacttt
      301 ttgcagtcgg gcatccttgt acattaatcg taaacgtttg actcggagaa acatcaccta
      361 catttaaaaa ttcattagca tcataagtgc ccatttttac cgactgatct ttacttccgg
      421 tagtaatgtc acagcttttt gccaacaaaa tcccagtaaa tgtaacaaca actgaatcat
      481 tggcctgccc tgagagcgga aaagcaaaaa aagcgaaaag aataaaaaat acagaatatt
      541 tagagaatat taaattcatt agcggcactc cagagtagca tagctaacag gattattcag
      601 ttctgtttca gggagcttgt aattagcctg acatttttgc tgaacaccat ctccccactg
      661 gatattaata acaccttcta aaggcaatcc tgagagataa agttgtccat tatcgccgac
      721 aatcccggta atttgactac tatttttaat ctgtgcgctg gctgtagcac cgaatgggac
      781 tgatttatta cgataacgaa ctgtgaacaa accacgaata ccgatatgag cagcaaactc
      841 tgcacgaact aacgcacctt ttcccggaat aacttcagtg gaagtgatat ccagttcagt
      901 atgcatattc attgacatag gatctaatga aatattatta cgtctatatt ctgttgcata
      961 aggaacaact gcatatccac gccagtcagt ggatattcct gtcccccgct ggacatccac
     1021 attattcgct ccaggcgctt ttatcaaaac attagtttca cctaatggtt gactcaacgt
     1081 aagaccattt tcatgtacaa caagagcacc actgatgcca tagtttatct gttgattacc
     1141 ttcattatcg taactgtagg cggaattcaa ggaaccatat gccccacgat aattaacacc
     1201 aacagaacca ctactgctat tatcctgatt agcaaaacca tgttgtatgt tataactgag
     1261 attatgctgc tccaacagag aaccagatac ccccgtatat tggtttacct gtccgtggtt
     1321 atcctgacta taaccatacg ttgcagtcat ggcattcgat gatgagcgcc catcattcgt
     1381 ggaagataac caattactta taggtaatga gacatttagc gagacaattt tatccgcatt
     1441 tgtccctggt gatttactgt aattccacgc aacatttaga tagataccct taatactggt
     1501 gttataacca acttggtata aagaatcttt tttatcagta ttccagtatg tttgctgact
     1561 agccgataaa tacatagacc cgtagttacc gaaaggttgc gatacactag cctgaaattt
     1621 tccacgtttg ctataacgca agttataaaa atcattccag ttataaacgt aatctttatc
     1681 atctaacgtt tttggatcaa caacagtccc tgacatttgt tggtatgtcg tatcacttaa
     1741 agtgtaaaag ccctgggttg aataacgata gccaataata tggaatgctg ttcctgtctg
     1801 aactaacgat ttggaataaa gaaaacgata agattgccct tgatgtttgc tcccatccac
     1861 aagcgaactg ttagcctgag taacatcaaa cgatgttgca ccaaatacac ccagattcag
     1921 gccaaggccg agcgccaacg cccggtaatc ctcagagaat tgtgccccac cataagccgt
     1981 aatatcccag ggtaaacccc aaattaattc cccctgaaat acataaggac ttttctggtt
     2041 atagctattg cttcgatatt ggccaaccgt agtagaatat tttgaatgac cttctcgttg
     2101 caggatgggg acagcggcat aaggtacggt atagacagtt tctgaattat cagactcttt
     2161 gatggtaact tccagatctc ccgcagatga tgtcgggtta agatcgctaa tctcaaacgg
     2221 tcccggcggc atataggttt gataaatgac ataaccattc tgtttgattg ttacctgagc
     2281 gcgacttttc gcaattccgc gcaccgtggg agcgaaacca ctttgactat cgggcaacat
     2341 attttcgtca gattccagtt ttatgccacg aatactaaca ctgtcgaaaa catctgatga
     2401 tgaccatgta tctcctagcg ttaattcact tctgaaagga ataataacgc gctgcaacgt
     2461 actactgata tgctccagtt tacctgctga attactccca cgactccagg ttgaataatc
     2521 gcgaaaacgc catgcaccaa tattgatccc agcgcgaaga tttaaataat agttatttga
     2581 agagtaatca ccacgatcgt tattaccaga aaagtcataa ttcagcaaaa aagctgtaat
     2641 accgttatcc cactgttctg gtggaatata tccgcgggcg tcacgtttta ttatcgcctg
     2701 cggaaaggtc agtttaagtc tctgtgcatc cagctccggc atgtaatgca catcaggaaa
     2761 ccagatacgc aaatcaaggc aactattttc agataaagcc aaagcctgct ctggcagcgc
     2821 attcttgtta attccaaggc ttaataagtc agcaagtgaa aggcagggaa tcagttgatt
     2881 ctcatcgtct gctttaaaag ttacagtacg agagagtaaa ggggaatcat tgacccaaac
     2941 ttcgacttca tagctaccag gaagataagt catccctttc tcgaaacggg ataagtcagc
     3001 aacagcttca gtatcacccg acagaaaagc agggttaaag cgcattcccg cgattgaaca
     3061 tgcaggcata ataaccagaa gaagcggtac aacccgtttc atctgctttt gtgccgccat
     3121 ccaatattcg catcttttga gctttaggtt catatccctg acctgtgttg catctgaact
     3181 atcccgttgt tactgcatta ttcctttctg ccgctccgtc gtagcgccaa agtcattaat
     3241 tgtttgataa gtaatttcac cagccaacgt cactgccgac gagaccgtaa tatcgctttt
     3301 cggtggtaca aacgtattgg gcaatttact cccttgtact ttaattcctg tcagtgtcag
     3361 gtaataaggc gaaggattga taacttttag ttgtttgccc tcatacttaa accgcaacat
     3421 atttcgtgct tttcctggtt gcacaggtag attttccggg cgataaaata gcttgatttt
     3481 gttttgtaat gccaactgta aggtattctt ctgtgtattt ttctcatctg ttggcggaat
     3541 tgctttaaca ttcatccaat acaaagtttc acgatcggtc ggcaaattag ttttaccaag
     3601 aaacatgata cgtaatgaag cttctttacg cgttggcaac ataaagaggg gaggagtgat
     3661 aacaaaatcc tggctctttt tttccgtttt actgtcatca acccatgatt gaattaaata
     3721 actggtgttg tcaccgctat tttgcacact gagtgttatt tgcttacttt ctgccggata
     3781 aatcaatcgc gttacgccaa ggccaaaccc gcccgcggac gcattattaa taaataatat
     3841 cgatagtaaa aaaagaatcc ctgagcgtat aatattttgt gatactttta attcatccct
     3901 gaatccataa acccattttt tatttccaat aatacatttc atacagcatc cttgttgatg
     3961 aaataacacc tgttattcat actctattaa ataatttcaa tctcaggtta caactttttg
     4021 taacctgaaa tcgtatttat atttactcgt acagcaccac aaaacttgct gttgacttaa
     4081 ctacaccagg tgcaacagtg ctaccgtttt gagcataacc tgcagtaaag gccgcgctga
     4141 aatcatgggt cgtagcattt cctggaattg tataagcggc ggtggaggcg ctaccctctg
     4201 ccgcagtagt attcaggtta atcagaccat tattcgtcgt acctttggag taaattcgaa
     4261 tccctacacc agtcgccccc gcattgccgc tgttgtcagc atccgttgat ccttctactt
     4321 cgttagcgag cgttttatta gcgctatctg cggtaccggt aaagcggaac tcaattttct
     4381 gatcctctgt attgctacat tcggtcagat taatattgaa atcggtctgg ttgctcgccc
     4441 cctgtgcttc tatgtcgata ccagtgaaaa ggttggaagg cacaaccggc aggacaactt
     4501 caatctgttt tgaatcggtt gcgaccttac aggtagcttt cactacacgg ccagtaaagc
     4561 ggattttccc ccccattgaa tcaacgttgt catccatatc agtgagcctg ggataatcaa
     4621 cggtgctcca gtcagtatcc gctccaccaa attgtgctgc atcgccgggt gctgcggtta
     4681 ccgcagtaga catcagcata cctgatgcca gtaatactgc ggataaatta gataaattga
     4741 atttcattta tacctttcct tttgttaa
//
LOCUS       DAZUCC010000284         4752 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZUCC010000284 DAZUCC010000000
VERSION     DAZUCC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.286"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..717
                     /gene="nanC"
                     /locus_tag="ACHY09_004682"
     CDS             1..717
                     /gene="nanC"
                     /locus_tag="ACHY09_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFS3887541.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            737..1843
                     /gene="nanM"
                     /locus_tag="ACHY09_004683"
     CDS             737..1843
                     /gene="nanM"
                     /locus_tag="ACHY09_004683"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFS3887542.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLAPVASPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRVYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1908..2888
                     /gene="nanS"
                     /locus_tag="ACHY09_004684"
     CDS             1908..2888
                     /gene="nanS"
                     /locus_tag="ACHY09_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFS3887543.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTCDYSSHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILRFWRER"
     gene            complement(2896..3546)
                     /locus_tag="ACHY09_004685"
     CDS             complement(2896..3546)
                     /locus_tag="ACHY09_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS3887544.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFSPEELKALMDANKSN"
     gene            3683..3826
                     /locus_tag="ACHY09_004686"
                     /pseudo
     CDS             3683..3826
                     /locus_tag="ACHY09_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4534..4617)
                     /locus_tag="ACHY09_004687"
                     /pseudo
     CDS             complement(4534..4617)
                     /locus_tag="ACHY09_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025205684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1374 a         1016 c         1120 g         1242 t
ORIGIN      
        1 atgaaaaagg ctaaaatact ttctggcgta ttattactgt gcttttcgtc accattaatt
       61 tctcaggctg cgacactgga cgtacgtggt ggatatcgta gcggaagcca tgcctatgag
      121 actcgactca aagtcagtga gggatggcaa aatggatggt gggcaagcat ggaaagtaat
      181 acctggaata ccattcatga taataaaaag gaaaatgccg cactcaatga tgttcaggtt
      241 gaagttaatt acgcgattaa acttgatgat caatggacgg tgcgcccggg aatgttaacg
      301 cattttagca gcaacggcac acgctacgga ccctacgtaa aactgtcctg ggacgcgaca
      361 aaagatctta attttggcat tcgctatcgt tacgactgga aagcttaccg acaacaagac
      421 ttatccggtg atatgtctcg tgataacgtt catcgttggg atggatatgt cacttaccat
      481 attaatagtg atttcacctt cgcatggcaa acgacgctat acagcaaaca gaatgattat
      541 cgctatgcaa accacaagaa atgggcgacg gaaaatgcct ttgttctaca ataccatatg
      601 acgcccgata ttacgccata catagaatat gactaccttg accgtcaggg tgtttacaac
      661 ggcagagata atttatcgga aaacagttat cgcattggtg tgtcatttaa actgtagtag
      721 acaggagaca gtcacaatga ataaaacaat aacggcgctt actatcataa tggcttcatt
      781 tgccgcaaac gcgtctgtat taccggaaac tcctgtgcca tttaaaagtg gtaccggagc
      841 aattgataac gacactgtct acattggttt aggtagcgca ggtacggcat ggtacaagct
      901 ggatacacag gccaaagata aaaaatggac agcgttagct gcattccctg gcggaccgag
      961 agatcaagca acctctgcat ttattgatgg caatctgtat gtgtttggcg gcattggcaa
     1021 aaacagcgag ggcttgactc aagtatttaa tgacgtacac aaatacaacc ccaaaaccaa
     1081 tagctgggtt aaattgatgt cgcacgcgcc gatgggcatg gcgggccatg tgacttttgt
     1141 acacaacggc aaggcttatg ttactggcgg tgttaaccag aatatcttca atggctattt
     1201 tgaagatctc aacgaagctg gaaaagattc aaccgctata gataaaatca acgcccacta
     1261 ttttgacaaa aaagcagaag attatttctt caataagttt ctgttgtctt ttgatccctc
     1321 aacacagcaa tggagttacg ctggcgaatc tccctggtac ggaacggctg gtgcggcggt
     1381 tgtgaataaa ggtgataaaa cctggcttat taatggcgaa gccaaaccag gattgcgaac
     1441 ggatgccgta tttgaacttg acttcaccgg tgataattta aaatggaata agcttgctcc
     1501 cgtcgcatca ccagatggcg tcgctggcgg ttttgcgggg ataagcaatg attctcttat
     1561 atttgccgga ggggccggat tcaaaggttc gcgggaaaat taccaaaacg gtaagaacta
     1621 tgcgcatgaa ggcctgaaaa aatcatatag cactgatatt catctttggc ataacgggaa
     1681 atgggataaa tctggtgaat tatcgcaagg tcgggtctac ggagtatcat tgccctggaa
     1741 taatagttta ttaattattg gcggtgaaac tgcaggcggc aaagcggtga cggattcagt
     1801 tttaatttct gtgaaggata ataaagtcac agtacaaaac taacgcttca gggcctcggt
     1861 aaaggggccc tgctatcaat ttgtcatttg tattacgcga aaattatatg aacgcaataa
     1921 tatcgcccga ttattactat gttcttaccg ttgctggtca gtctaatgcc atggcgtatg
     1981 gcgaaggact gccattaccg gacagggaag atgcgcctca tcccagaatt aaacaattag
     2041 cgagatttgc gcatacgcat cccggaggcc cgtcatgtca ctttaacgac attattccac
     2101 tgactcactg cccacacgat gttcaggata tgcagggtta tcaccatcct ctggcaacaa
     2161 atcatcaaac acagtacggc accgttggcc aggcactgca tattgcacgg aaattactgc
     2221 cctttattcc tgataacgca ggggttctca tcgttccgtg ttgccgtggc ggatcggctt
     2281 ttaccgcggg cagcgaaggg acatattcag aacggcacgg agccagccat gatgcttgtc
     2341 gttggggaac ggatactccg ctgtaccagg atttagtcag cagaacgcga gccgcactgg
     2401 caaaaaatcc gcagaacaaa ttcctcggcg tatgctggat gcaaggcgaa tttgacttaa
     2461 tgacctgtga ctactcgtca caccctcaac actttaatca tatggttgaa gcctttcgta
     2521 gggatctaaa acaataccat tctcagctta ataatattac tgacgcaccg tggttttgcg
     2581 gcgataccac ctggtactgg aaagaaaatt tccctcatgc gtatgaagcg atttatggca
     2641 attatcaaaa taatgtttta gccaatatta ttttcgtcga cttccagcaa caaggtgaaa
     2701 gaggactgac gaacgcgcct gatgaagatc cggacgattt aagcacggga tattacggtt
     2761 cagcgtaccg gtcaccggag aactggacga cggcactgcg aagcagtcat ttcagcgcgg
     2821 cagcccgtcg ggggattatt tctgacaggt ttgtagaagc aattttgcgg ttttggcgcg
     2881 aaaggtgagc gttcattaat ttgatttatt tgcatccatc aacgctttta actcctccgg
     2941 tgaaaacggt ggtttgcgtc ggtgcagcat cgcatggcaa ttagggcaca caggcagcaa
     3001 atcattaact ggatccagct ggtaatcctc tttaatggct gaaagcggca ccaggtgatg
     3061 catatggata aagccttcag cgatatcacc atagatcttc accagatcaa cgccgcatac
     3121 gttgcactgg catccatgat gttcgaccgc tttatctcgg gcctttttat cgcgttcata
     3181 gcggtttact gtcaccttta tcgccgcacc ttcaaggtat gtctcctgcg acggcaactc
     3241 atccgggaac agaacgtcgg ctatgggagc cgcagcggct ttatcctgat gaatatgaaa
     3301 tccctgctct atgagtagtt tgacgcattt agattttatt ccgccggtga aatccgcagg
     3361 ggtaaattgc gtccccgtca tcaatgtcgc tgcgatgcca gcaatcgcct tcgaggggta
     3421 acgtcggcca tgatattcaa tctcatacaa ccgcgcaggc tggaactgat gagtttctcc
     3481 cgcatcccat aaatggatag cttcgatgat ataccgccgc ggaagatgat cagggagttt
     3541 attcatggag aatgtcctta tacgttctca gaacaggcag caaaaaaaca aatgagatca
     3601 aggtaatata tcttcacttg caggcttgaa cagcagaaaa tttgcgagca tgatcgcaat
     3661 tatttcactg ccagggatat caatggcttc cagtaaatcg ctacagcagg caattgccaa
     3721 tataaaaatc tggcacaagg gtgaacagcg cgcgccgcat aagccattgt tattgctata
     3781 cgtattagcg ggatacctga atggacatcc gcgccttttc gattattgta gtggaacagt
     3841 ctacgaggat gcagttcgaa gtgagtaagt aaaggttgct ggaaacgatc aatgccgcca
     3901 tacaatgatt tttgaggatc aacaatgagt aataaaaact acgaaagtca ccgcaaagcg
     3961 attgttagta aaggtatacc acccgctcta ttaaataggc tcaccaattc ggatgttcag
     4021 gtgatcaata ccttcttaac acgagtgagc aaactggaac tatctcaaca agagaaagac
     4081 tggatcataa aaatcatctc tatggtttag acaatttcag ccttgtagcc tttcaagggt
     4141 tttgtgtggg tacaaacatt atcgaatcgg gatatagccg cagctgtagc tgtatccaca
     4201 tcagtaagtt gatgtaggtt gacttaggtt gagcgaaaaa tagcgaaaag ccttgtgtgg
     4261 cgcggatttc agtcataaaa aaacgtccgt tgacgtctat tgatgttccg atggtgcgaa
     4321 ggtcgaaggc cggactcgcc tttgcgttta attcgttgaa aaataatggt ttgtgagatt
     4381 ttattttgag gatttgcccc caaaatggga cataaaattg aatactgggt ggaagatcac
     4441 aaagttgcgt aattattttc caggtccgaa acgcattcaa gagcaaggcc tctcccggca
     4501 ctgcgtcagt cgggatgcta aatctcccac atgtagccct agtaaatcgc gccagaatgt
     4561 caggctagtt tgcaaattac tgacggcgag cgttaagtgg ttcagagatt gaagcatagg
     4621 atgcctattg atggttaata atttaaccaa acactaatag ctaataaaac cccacatgga
     4681 aagagatttt ttgcgggtct tgttttgtaa taaatttctt ggcaattact ttatttcata
     4741 tatatattag ca
//
LOCUS       DAZUCC010000285         4715 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZUCC010000285 DAZUCC010000000
VERSION     DAZUCC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.287"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..1171
                     /gene="yiaY"
                     /locus_tag="ACHY09_004688"
     CDS             20..1171
                     /gene="yiaY"
                     /locus_tag="ACHY09_004688"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFS3887545.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAEGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMVGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDIPVLATNALKDACGFTNPIQATHEEIMAIYRAAM"
     gene            1301..2596
                     /locus_tag="ACHY09_004689"
     CDS             1301..2596
                     /locus_tag="ACHY09_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFS3887546.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKHATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            2704..4242
                     /gene="aldB"
                     /locus_tag="ACHY09_004690"
     CDS             2704..4242
                     /gene="aldB"
                     /locus_tag="ACHY09_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFS3887547.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4461..4616)
                     /locus_tag="ACHY09_004691"
     CDS             complement(4461..4616)
                     /locus_tag="ACHY09_004691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887548.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGTPMVIYGLVRP
                     LTIETRD"
BASE COUNT         1194 a         1135 c         1251 g         1135 t
ORIGIN      
        1 acactttcag gagtgtgtta tggcagcttc aacctttttt attccttccg tgaatgttat
       61 tggtgctgat tcattgaatg atgcaatgaa tatgatggca gattatggat ttacccgtac
      121 cttaattgtc accgacaaca tgttaacgaa attgggtatg gcgggtgatg tgcaaaaagc
      181 actggaagaa cgcaatattt ttagcgttat ttatgatggc acccaaccta accccaccac
      241 ggaaaacgtc gccgaaggtt taaaattact taaagaaaat aattgcgata gcgtgatctc
      301 cttaggcggt ggttctccgc acgactgcgc aaaaggtatt gcgctggtgg cagccaatgg
      361 cggcgatatt cgcgattacg aaggcgttga ccgctctgca aaaccgcagc tgccgatgat
      421 cgccatcaat accacggcgg gtacggcctc tgaaatgacc cgtttctgca tcatcactga
      481 cgaagcgcgt catatcaaaa tggcgattgt tgataaacat gtaactccgt tgctttctgt
      541 caatgactca tctctgatgg tcggtatgcc gaagtcactg accgccgcaa cgggtatgga
      601 tgccttaacg cacgctatcg aagcgtatgt ttctattgcc gccacgccga tcactgacgc
      661 ttgcgcactg aaagccgtga ccatgattgc cgaaaacctg ccgttagccg ttgaagatgg
      721 cagcaatgct aaagcgcgtg aagcaatggc ttatgcccag ttcctcgctg gcatggcatt
      781 caacaacgca tctcttggtt atgttcatgc gatggcgcac caactgggcg gtttctacaa
      841 cctgccacat ggtgtatgta atgccgttct gctgccacac gttcaggtgt tcaacagcaa
      901 agtcgctgct gcgcgtctgc gtgactgtgc tgctgccatg ggcgttaacg tgacaggcaa
      961 aaatgacgcg gaaggcgcag aagcgtgcat caatgccatc cgtgaactgg cgaagaaagt
     1021 gaatatcccg gcaggcctgc gtgacctgaa cgtgaaagaa gaagatattc cagtgctggc
     1081 aaccaatgcc ctgaaagatg cgtgtgggtt tactaacccg attcaggcaa ctcacgaaga
     1141 aattatggcg atttatcgcg cagcgatgta atcatcattc ccacaatggc tgtctaattg
     1201 tcagccattt tttaaactat ctcaaaattt gcgatttttg agatagtttc tctttcctgc
     1261 tatacactat ctcaatattg cgcttttttg agataagcac atggctatca aatcacctcc
     1321 cggacttatt cctttatcac acttatcggg tgaggaactc ctggcccacc tgcgctttaa
     1381 ccgggttacc gatgaaaaag gacgttatct gccttttgat gagctgcagt accgcatcaa
     1441 aaaaggagaa aacgtcgatg tggcgtggac gcttacccgt ctggcgcgca acgcggcgat
     1501 ccaacgaatt aattattgca atgaagcggg tgaacaggcc ggattcaaca ttacgccggt
     1561 gattgcagaa gcgtgcgagc ttgttgataa acatgccact gcactggcgc tgaaagatca
     1621 gactgaacgg ttacgcgggg cgggcgccga gttaagtcag ttacgcctgg aagagccgat
     1681 caccagttcc caactggaag gtgcgaatac tacaacgctg gtggcgagaa aaatgctgga
     1741 aacagggcgg tcgccacgca ctgaagacga gcatatgatt gcgggaaatg cccggctgat
     1801 ggcggaaatt ccgcacttac tcgcagaacc gctgacacct gcgcttattc gccagttaca
     1861 tgccattggt atgggtggaa tcaatgacgc gaaatatcgt ccgggagaat ttcgtgaaac
     1921 agatgatgtt gtgatcgccg actatgatgg caatatagtg catcaaccgc ccgctgcggc
     1981 attgttaccg gaacggctgg aaaaagtttg ccagtggtta aacagccatg aagggtatat
     2041 tcatccgttg gtccgtgcct gcattctgca ttttatgctg gcccatgaac atccgtttcg
     2101 cgatggcaac gggcgcacca gtcgggcgct gttttactgg tatatgctga aatcgggcta
     2161 cgatgtcttt aaatatattt ccatcagccg tttgcttcat gctgcaccgg tcaaatatgc
     2221 cgccagctac cagtacactg aatcagatgg tatggatctg acttatttcc ttgaatatca
     2281 ggcgggtgtc attaaacgtg ctctgcaaaa ctggcagcag catatcgatg aaattacaca
     2341 acgaagcgca aaactcgata gtgtgctgtt tagctctggt gtgcttaaac gcctgaatcc
     2401 gcgccaggta acgctcctga atgtgatgct cgccaatccg gggaaagagt acacagttgc
     2461 tgaaattagc gcgagcttgg gcgtctctga taatacggct cgagctgatt tacggacaat
     2521 tgttaaagaa ggattcgcgc aagagaaaag aattaataac cagcaagccg tgtacgtcgc
     2581 acactacccg ctgtagcttt tgcccgtaaa ttcgtgatag ccgtcgtaaa gctgttaccg
     2641 actggcgaag atttcgccag tcacgtctac ccttgttata cctcacaccg caaggagacg
     2701 atcatgacca ataatccccc ttcagcacag attaagcccg gcgagtatgg tttccccctc
     2761 aaattaaaag cccgctacga caactttatc ggtggcgaat gggtagcgcc tgccgatggc
     2821 gagtattacc agaacctgac gccggtgacc gggcagctgc tgtgcgaagt ggcgtcttcg
     2881 ggcaaacgag acatcgatct ggcgctggat gctgcgcaca aagtgaaaga taaatgggcg
     2941 cacacctcgg tgcaggatcg tgcggcgatt ctgtttaaga ttgccgatcg aatggaacaa
     3001 aaccttgaac tgttagcgac cgctgaaacc tgggataacg gcaaacccat tcgcgaaacc
     3061 agtgctgctg atgtgccgct ggcgattgat cattttcgct attttgcctc gtgtattcgg
     3121 gcgcaggaag gtgggatcag tgaagttgat agcgaaaccg tggcctatca tttccatgag
     3181 ccgttaggcg tggtggggca gattattccg tggaacttcc cgctgctgat ggcgagctgg
     3241 aaaatggctc ccgcgctggc ggcgggcaac tgtgtggtgc tgaaacccgc acgtcttacc
     3301 ccgctttctg tactgctgct aatggaaatc gtcggtgatt tactgccgcc gggcgtggtg
     3361 aacgtggtca acggcgcagg tggggaaatt ggcgaatatc tggcgacctc gaaacgcatc
     3421 gccaaagtgg cgtttaccgg ctcaacggaa gtgggccaac aaattatgca atacgccacg
     3481 caaaacatta ttccggtgac gctggagctg ggtggcaaat cgccaaatat cttctttgct
     3541 gacgtaatgg atgaagaaga tgcctttttc gataaagcgc tggaaggctt tgcactgttt
     3601 gcctttaacc agggcgaagt ttgcacctgt ccaagccgtg ctttagtgca ggagtctatc
     3661 tacgaacgct ttatggaacg cgccatccgc cgtgtcgaaa gcattcgcag cggtaacccg
     3721 ctcgacagcg tgacgcaaat gggggcgcag gtttctcacg ggcaactgga aaccatcctc
     3781 aactacattg atatcggtaa aaaagagggc gcagacgtgc tcactggcgg gcggcgcaag
     3841 ctgctggaag gtgaactgaa agacggctac tacctcgaac cgacgatttt gtttggtcag
     3901 aacaatatgc gcgtgttcca ggaggagatt tttggcccgg tgctggcggt gaccaccttc
     3961 aaaacgatgg aagaagcgct ggagctggcg aacgatacgc aatatggcct gggcgcgggc
     4021 gtctggagcc gcaacggtaa tcttgcctat aagatggggc gcggcataca ggcagggcgc
     4081 gtgtggacca attgttatca cgcttacccg gcacatgcgg cgtttggtgg ctacaaacag
     4141 tcgggcatcg gacgcgaaac ccacaagatg atgcttgagc attaccagca aaccaagtgc
     4201 ctgctggtga gctactcgga taaaccgttg gggctgttct gatataagaa gctggtcgca
     4261 ttgggtattc attgcctgat gcgacgctta cgcgtcttat catgcctacg gggacctgac
     4321 cgtaggccgg ataaggcgta cacgccgcat ccggcataaa caaagcgcac gttgttacaa
     4381 tttacgtttt aaacctacgt gcgcaataac ccagactacg tcaacagaag tgcgtttctg
     4441 gggcgtgttt tgccaggagt ttaatctcgc gtttcgatgg tgagcgggcg aaccaggcca
     4501 taaatcacca ttggcgttcc gaggccaatc agcaataaac ggtagataat tgaaggttta
     4561 atacgcttaa tctgtactgt ggatgtttta ctggagttat tcattagttt ggccaatagt
     4621 tccattattt caatatgata agcctcgcac tgttgaaaag ccaggctgca cacttcagaa
     4681 agtatataaa aaacaatatg tcgttatata aataa
//
LOCUS       DAZUCC010000286         4704 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZUCC010000286 DAZUCC010000000
VERSION     DAZUCC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.288"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            264..1820
                     /locus_tag="ACHY09_004692"
     CDS             264..1820
                     /locus_tag="ACHY09_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFS3887549.1"
                     /translation="MHQYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILAT
                     LFWHTPVQDLGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMNILRNVLAAVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLAAFNILLPFVIVAIIGEGLKAIRGVGFMTL
                     MCGVSTLIPQYLVAVYLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIETSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSTVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGWIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            1870..2589
                     /locus_tag="ACHY09_004693"
     CDS             1870..2589
                     /locus_tag="ACHY09_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFS3887550.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQNSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            2600..4015
                     /locus_tag="ACHY09_004694"
     CDS             2600..4015
                     /locus_tag="ACHY09_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFS3887551.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSLAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREEATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNGVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFREVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKELPYACSLCTACNGVCPVKIPLAQLIQKHRQVMAES
                     EITPSTERRITRIFNYVNSHPALWKVGMIAGAKTASLCIRNGKTPISPGAIKEWSVAR
                     DLPSPDGESFRSWFKRHKKGG"
     gene            4020..>4704
                     /locus_tag="ACHY09_004695"
     CDS             4020..>4704
                     /locus_tag="ACHY09_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFS3887552.1"
                     /translation="MENREEFLVAIARKLGRPVRRIPEAMPEPVSTLATTRLTELTMD
                     QRCEAFIKFASEVMLAKCVLVSPEDAPSKALDICWTFGSGPVIISNDQRLFDTGIAPL
                     LQKEMGAVLWTPEKGEENIHLAEKAKTGVVFAEYGLTESGGVVLFSAPERGRSVSLLP
                     EASVFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLII"
BASE COUNT         1246 a         1021 c         1152 g         1285 t
ORIGIN      
        1 tagttgaaca aagttcattc aaacaggttc ttttaataac atttagtcaa ataaaataac
       61 aataaaacga ttcagcaatc atttttcata acaaaaacaa attataaccc ccttccctgt
      121 ttgtggacga gactcgttct ctccctgcga gacaagcgta aattggtcac accgacggtc
      181 gttgcctgaa aattacaaat tctcgcctga cagggcaata acaacagtaa tgatgctgaa
      241 tttgttttcg ttgaggaaat gacatgcacc aatacctgct ttttttactg ggacttaccc
      301 cacttctggc aatgatcatt ttgattctga aagtcaaaat gcccatacat tatgccgtac
      361 tcagcacact ctttattaca ctgattcttg caactttgtt ctggcacaca ccagttcagg
      421 atctgggttc tgctgttgta tatggcgcaa tcaaagggct gtggccgata gtgatagtca
      481 tacttgctgc catttacagc tataacctga tgaatgcgac aaaaagcatg aacattctgc
      541 gaaatgttct ggccgctgtc agtgatgata aacgtatcca ggtgcttctt atctcatggt
      601 gttttggtgg atttcttgag gcggcggccg ggtacggtac agctgtagca atccccattg
      661 gtatactcat tgcactgggc tttaaccctc ttaaagccgc aattgcatca ttggttgcta
      721 atacagtacc taccgcgttt ggtgctgtcg gcatacctgt atcagtactt gctgaacagg
      781 taaatttacc cgtaacaacc ctcagcggga cgattattct tcagctggca gcgttcaaca
      841 ttctgcttcc cttcgttata gtcgctatta ttggggaagg cctgaaggct atacgaggcg
      901 ttgggttcat gaccttaatg tgcggtgtga gcacattaat cccacagtac ctggtggctg
      961 tttatcttgg cgcagaactt cctgctttcg cgggaagtct ggtcagcctg atcgtgatag
     1021 tctttatggc aaaaaaccgt aaagaaaaaa cagctcctga atttcttatt gagacttcag
     1081 cagatagcgc cagcgcactt tcatactctc gtggggcgtt gttacgtgcg tgctcaatct
     1141 acatcctgat ttttctgttc attcttcttt gctctccact gttcccgacg gtaaaaagta
     1201 cagtgtctca gattgcctcg tcactcagct tctctctgag taacggcagc aatctgtcga
     1261 tgaagattga atggatcgca acccccggtg ttctgattat cattgccagt gttattggcg
     1321 gctggataca gaaagcatcc ctgtcatgcc tgttaaatgt atttgtcagt acggtaaaac
     1381 agctcaaaaa ctccattatt gccatttctg cgattgttgc tatggcaacg gtaatggatg
     1441 tttgtgggtt gattggcact ctggcacaga gccttgtaga tctcactggt ggggcctacc
     1501 tcttcattgc tccggttatc ggcgctcttg gtacatttgt tactggcagt gacacaaact
     1561 ccaacattct gtttggtaaa ttgcagacca ctgcggctga aaaattgcat gtggacccac
     1621 tctggctggc tgccgctaac acatctggcg caacaggcgg caaaatgatt tctcctcaga
     1681 gtattgctat cgctgtttca gccacccgta tggatggtca ggggagcgta atcatgtcag
     1741 ggacactgaa gtactgcttt gcttatattg ttattcttgg actgaaagta ggccttgtct
     1801 accacctgtt catggcctga gaaaaaataa caaacggacg tggaatatta atcaacagga
     1861 gaaatatctg tgaatgttaa ctttttcgtc acctgcattg gtgatgcatt taaatcccgt
     1921 atggcacaaa acagcgtgct gttactggag caattgggtt gcaatgtcca tttccccgaa
     1981 aaacagggat gttgcggtca gccagcgata aatggcggtt atgttcgtga tgccattcct
     2041 ggtatgaaaa atctcatatc ttcgctggaa gagaatgact ggccggtagt atctcctgcc
     2101 gggtcatgca cttacacgat aaaaaattac gctcgctatc tcagtgaaga acctgagtgg
     2161 gcggcacgag ctgaaaaagt agctgaacgc ctgcatgatt tgacgtcatt tatagtaaat
     2221 catcttggca tcacagatgt tggggcaaga ctcaatggtc gcgcagtgta ccatccttca
     2281 tgcagccttt cccgtaaact gggtgtaacc cgagaaccgc ttttgttgct tgagaatgta
     2341 cggggacttg agctgctgcc gtttcacgct caggaaacgt gctgcgggtt tggcggcacg
     2401 ttctctgtaa aaatggcgga gatctctggt gaaatggtga aagagaaagt cgggcatatt
     2461 atggatgtta aacctgattt tcttatcggg gctgacgcca gttgcctgct caacatcggc
     2521 ggtcggctta gccgtgaagg gcatcctgta aaagtgatgc atattgctga agtcctgatg
     2581 cacagatgag gtaaacagaa tgtctttgaa aacaagtgat atcaggttta aagagcgtat
     2641 ccgtattcag attcaggacc cgatcatgcg aaaagctgtg gctaacgcac aggagcgcat
     2701 agggactaat cgacagaaaa tggttgatga actggggcac tgggaagaat ggcgttcact
     2761 tgctgaacag attcgtcggc atgttctgga aaatcttgat gcttatttgt atcaattgtc
     2821 cgaaaaagtt actgcgaatg gcggacatgt atattttgcc cgtaccagag aggaggcaac
     2881 cagttacata ctgaaggtgg cgaaagaaaa aaacgccagg aaggttgtta aatccaaatc
     2941 aatggtgact gaggaaattg gtatgaacgg cgttcttcag aaagccggga taccagtgat
     3001 agaaacagat cttggtgaat atattcttca gctggcgcat gagccgccat cgcatgttgt
     3061 tgtgccggca atacataaag atcgccatca aattcgcaga gttttgcatg aacatctggg
     3121 gtatgacggc cctgaaactc cagaagcaat gacattgttc atccgtgaga aaatcagaca
     3181 ggattttctg agtgctgaag ttggcgtaac agggtgtaac tttgccgttg cagaaacagg
     3241 ctcagtttgc cttgtgacaa acgaaggtaa cgcacgtatg tgcaccacgt tacctaaaac
     3301 acatattgct gtgatgggta tggagcgtat tgcaccaaca ttccgggagg tggatgttct
     3361 tatcaccatg cttgcaagga gtgctgtggg agcaagactg acaggttata acacctggct
     3421 tacaggtcca agagaagaag gacatgttga cggcccggaa gagttccatc tcgtcattgt
     3481 tgacaatggg cgttcagagg ttctcggctc agaattcagc gatgtattgc gctgtattcg
     3541 ctgcggtgcc tgtattaata cctgtcccgc ttatcgccag attggtggac atggctatgg
     3601 ctcaatctac ccgggcccaa tcggagcagt tatttccccg ttgcttggtg gatatgaaga
     3661 ctttaaggaa ttaccttatg cctgctcatt gtgcactgcc tgcaatgggg tatgtccggt
     3721 aaaaatacct ctggcacaac taatacaaaa acatcggcag gttatggcag agtccgaaat
     3781 aacaccatca acagaacgcc gtataaccag aatattcaat tacgtaaaca gtcatccggc
     3841 attgtggaaa gttggcatga ttgctggcgc gaaaacagcc agcctgtgca tcaggaacgg
     3901 aaaaacgccg attagcccag gtgctattaa agaatggtct gtggccagag acttaccttc
     3961 tcctgatgga gaaagcttcc gtagttggtt caaaagacat aaaaaaggag ggtaacttta
     4021 tggaaaacag agaggaattt ctcgttgcta tcgccagaaa gcttggcagg ccagtaagac
     4081 gtattcccga agcaatgccg gagcctgtaa gtacgctggc tacgacacgt ttaaccgagt
     4141 tgacaatgga ccaacgctgt gaggctttta ttaaatttgc ctcagaagtg atgcttgcga
     4201 agtgcgttct ggtttcgcct gaagacgcac catcaaaagc attagacatc tgttggacgt
     4261 tcggttccgg gccagtcatt atcagcaacg atcaacgact gtttgatacg gggattgcac
     4321 cattgctaca aaaagagatg ggcgctgtcc tgtggacccc ggagaagggt gaggaaaata
     4381 ttcatcttgc agaaaaagca aaaacaggcg tcgttttcgc tgagtatgga ctgacagaat
     4441 ctggtggtgt tgttttattc tccgcccctg aacgagggcg ttcagtaagt cttttacctg
     4501 aagcgtctgt gtttgtttta cgcaaaagta ccattcttcc cagagtggct cagcttgcgc
     4561 aaaaacttca tgatatggct caggaaggac aacgtatgcc ttcctgtatc aatctgattg
     4621 gaggcccaag ttctactgca gatatagaac tgatcaaagt tgttggtgtg catggacctg
     4681 tacatgcaac ttacttgatt attg
//
LOCUS       DAZUCC010000287         4620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZUCC010000287 DAZUCC010000000
VERSION     DAZUCC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.289"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(43..1145)
                     /locus_tag="ACHY09_004696"
                     /pseudo
     CDS             complement(43..1145)
                     /locus_tag="ACHY09_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
     gene            1174..1449
                     /locus_tag="ACHY09_004697"
                     /pseudo
     CDS             1174..1449
                     /locus_tag="ACHY09_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419664.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2222..3199
                     /locus_tag="ACHY09_004698"
     CDS             2222..3199
                     /locus_tag="ACHY09_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS3887553.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3485..4225)
                     /locus_tag="ACHY09_004699"
     CDS             complement(3485..4225)
                     /locus_tag="ACHY09_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3887554.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGLRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALVGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4346..4576)
                     /locus_tag="ACHY09_004700"
     CDS             complement(4346..4576)
                     /locus_tag="ACHY09_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFS3887555.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWYYPNSP
                     KNDKTALHPLHYWLHEGSEQKKNPFPIWFYPR"
BASE COUNT         1137 a         1149 c         1086 g         1248 t
ORIGIN      
        1 aaaaggctgt ctgtaagata gacagcctgt cttatatatt atttaaatgc cccagaactt
       61 attcgctttt tccacaagcg tttcatcaat cagtgcaccg gtgacagcca tcagtaaacc
      121 atacccgata atgcctaaag cgcttccggt aagaatactg aagaccagag caacaagagc
      181 cgttgcggca ttacctgcta tgatggtttc tgttttaaca aaaaaggacg ccagttatct
      241 gtccgggcag ccttaccaaa ctcagtgatc cagtcagcaa aatttgtaat ttttcctgca
      301 tatcccagtc cgcgactgaa cctgttcaga ttagacgata tatcagacag cttcacagac
      361 tcaagggctg cggcaatcgc tgcacgatct tttgcattaa tttttttgtt aatgtcagtc
      421 cggtactttt catacgtttt taatgcctct tcaacattac gtattttctt ccctttagcc
      481 tgcccggcca tctctctggc taactgctca gcttttgcac catatttttc tgaaactgat
      541 ttcaggaact ctgttgtgaa attaattgcg tctttcgttg ccttcagttc atcctgtttt
      601 cttttctctt ccgctatctt ctgattgatg ccggcaagct ggttacgggt attctctttt
      661 tccttcaaca gggcattaag agcgtcattt gcatgctttt gctcatttgt tctggcactg
      721 aggttatttc tcaccgaatt taccgcagat tcagcagagg tgattttatt tctggcatca
      781 agcaatcttt gccgtaactt atcccattca gtaacttcag cattataaat tttcttgtca
      841 ccttccacat cctctgtcac caccggattc cggtcattca gaatattgcg tttatagtcc
      901 agaccggaat gagtcagaag ataattcagg tttgcacggt tatcgatgac tgcgctggtt
      961 gtgattttta tcgtatcggc aaatctcgga tcaccgctca caacaataga atgatttgtt
     1021 gatgaacgcc ccgggaattt ttcagaggaa actatcgttt tgccgtcagg cgtgtttttc
     1081 tgttgcgtca gctgttgttc agctctggtt aatgcattac gggccgtgtt aagcgattcc
     1141 gtcaccgaac tgagtttatt ccgggcctct gaaatggccc tgtcattccg tgtctgctgc
     1201 tcgagcagac gggcctgttg tgcatcaaga acggcaaggg ctgccccgcc ctttttttcc
     1261 agctctgaca accgcgtatt cagaatacct tttctgcgct cgtattcagc aagcatatca
     1321 gcggccttct tgtctgcgtt ctgcgcatcc ttctgccgtt tttcagcctg ttcaattaac
     1381 gacctggcct cgcggggaga tatacgaaga gaatcagcat cagcaaacag catccggctc
     1441 tctgtatgtg tcaacgccac gatgtttgac cgtcttatat cactggcgct gacaacacga
     1501 cagagttatc aggcatggtg tcaatcagtt cacaattttt ccatcggagc agcctaccac
     1561 cggtgataac ctgactttgc ctgtagtttg tatacacaat ccctggcatt gaaacacgca
     1621 tatgaggtgt acttcggaat atgaccgtgc gctcaggaag tacggcagat tccgtcaaaa
     1681 attacctgta aagacagact tcagaaaaaa cagtgctcgc cgcagaccgg tgaccgggcg
     1741 aagcccgcga accggcgagg aagcgcaata tatctgtagt tacaatgagc aggtacgccc
     1801 tgaagccttc ataatcggta gtgtatgttg tttttctgga atgatttatc cacatatctg
     1861 gaggtccgat ccagtaaaca gatccatgaa tgatcaacaa aggatccatt aaagatcccc
     1921 atatcgctgc aaaccttgtc actcatgggc cgggaccacg atcacataag ctgtggcatg
     1981 ttactgataa actgtaacat gctaatgata agctgtattc agtaatccat atactgaagt
     2041 aagttaatga cataaactat ggtcagtacg tcagactcag ttgttaaata caggctgcag
     2101 gtttttattc agtcagttag cggagttctg acacacgatt ttctgtttat tcttttactg
     2161 tccacaggct ggaggctttc tggaaaacga aaattcagac atcaaaaaac tgttcggcga
     2221 ggtggataag tcgtccggtg agctggtgac actgacacca aacaataaca acaccgtaca
     2281 acctgtggcg ctgatgcgtc tgggcgtctt tgtaccgacc cttaaatcac tgaagaacag
     2341 taaaaaaaat acactgtcac gtactgatgc cacggaagag ctgacacgtc tttccctggc
     2401 ccgtgcagag ggattcgata aggttgagat caccggcccc cgcctggata tggataacga
     2461 tttcaagacc tgggtgggga tcattcattc ctttgcccgc cataacgtaa ttggtgacaa
     2521 agttgaactg ccttttgtcg agtttgcaaa actgtgtggt ataccttcaa gccagtcatc
     2581 ccgcaggctg cgtgagcgca tcagcccttc cctgaagcgc attgccggta ccgtgatttc
     2641 gttttcccgt accgatgaga agcacacccg ggaatatatc acccatctgg tacagtcagc
     2701 ctactacgat actgagcggg atattgtgca gttacaggcc gatccccgcc tgtttgaact
     2761 gtaccagttt gacagaaaag tccttctcca gcttaaggcg attaatgctc tgaagcgacg
     2821 ggagtccgcc caggcactct acacctttat agagagcctg ccccgggatc cggcaccgat
     2881 atcgctggcg cggctgcgtg cacgcctcaa tctgaagtct cctgtatttt cccagaacca
     2941 gacggtcaga cgggcaatgg agcagttgcg cgagattgga tatcttgatt acacggagat
     3001 ccagcggggg cggacaaaat tcttctgtat tcactaccgg cgtccccggt taaaagcgcc
     3061 gaatgatgag agtaaggaaa atccgttgcc accttcacct gcggaaaaag tcagtccgga
     3121 gatggcggag aagcttgccc tgcttgagaa actgggcatc acgctggatg acctggaaaa
     3181 actcttcaaa tcccgctgaa tataaactgt agtcagtgaa gagcgctcct ttactgacta
     3241 cagcttatat taactggtgc agtgagtggt ctgctcactg cagtttatat tcttgttccc
     3301 tgcagtgctg tttgtagctg agctgccatc tgcctgtccc ttacgtaagt gatcccgtaa
     3361 cctgatgctg aggcattgct cccttcataa aacatgactt actcactaca gcttatatac
     3421 atgctccagc ttatgttatg tctgttctgc tgaccacagc ttatataagg aagcgcgtat
     3481 gccgtcacct caggggaggc agtgtacgca ggatctccgc agcatcccgc ccgtcaccgg
     3541 tgaaaggcac tgccagcgtg gcagccatat ccagcgcaaa cactctggta taaacctcca
     3601 tcgaacgtgg atccctgtga ccgaccagtg cctggatgac tttccggggc tggcggtgat
     3661 agagcatgtg catgatatag ctgtgccgga aggtgtgtgg tgtgaccgga atcgaaaagt
     3721 gaaccccgtc tgcttcggcc cgtctgacag cctgcttcag ccagttgcgc atggtttcgt
     3781 cggtcacggc ccataatggt tcacgacgcc ggggccgggt ggtgatcatc cagctttcca
     3841 tctgcctgac atagcttata tctgtcagcg gaaccaggcg gacttcatct tttggcgggc
     3901 gtccgcgtcg cgcacgcact ttttcggaca ggatccgcac aaacggtcgc agaccatcca
     3961 gatcaaatga ttccggcgtc agcatccggg cttcgccaat acgcattccg gtattccaga
     4021 gggtggcaaa cagcatatgg tgacgctgat ccggcatata gaagagaagg gcactgactt
     4081 ccggagccag caggtaggcc ggtgtggccc ccgtggaagt ggccattctt ctcagggaga
     4141 gcgctgttgc aaaatccacc cccggcgcaa tgggtaacag ggagacgcgt tctggtgaat
     4201 tctgcagggg aatgacattg ttcatgagtt atttttcacg tagcacacgc actgtttatg
     4261 gatacaggta ttctgacctg aacgcccgga tccttccacg acaataaatc gcagcgcgca
     4321 gatccttttg tttgcttatc ccacattatc gcgggtaaaa ccaaattgga aaaggattct
     4381 ttttttgctc agatccttca tgaagccagt aatgaagcgg gtgtagggct gttttatcgt
     4441 ttttgggaga attggggtaa taccaggacg ctaatgatct atcaatggat ggatgtaagt
     4501 gacaattgaa aactgaaaaa tgcgctatgt agattctgac agatttatgc acctgaaata
     4561 cgttcaaaat ccacatagcg taactctgat ttcccttacc agaaaaaaga gggggaataa
//
LOCUS       DAZUCC010000288         4612 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZUCC010000288 DAZUCC010000000
VERSION     DAZUCC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.290"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..636
                     /gene="hprR"
                     /locus_tag="ACHY09_004701"
     CDS             <1..636
                     /gene="hprR"
                     /locus_tag="ACHY09_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFS3887556.1"
                     /translation="QEWVTQGLSEAGYVIDAVSDGRDGLYLALKDDYALIILDIMLPG
                     MDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPFSFSELLARVRAQ
                     LRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLASRAGEIIPRTVI
                     ASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSFVAVKK"
     gene            636..1994
                     /gene="hprS"
                     /locus_tag="ACHY09_004702"
     CDS             636..1994
                     /gene="hprS"
                     /locus_tag="ACHY09_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFS3887557.1"
                     /translation="MKRLSITVRLTLLFILVLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGHGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDNREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICL
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGSLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(2101..2952)
                     /gene="hchA"
                     /locus_tag="ACHY09_004703"
     CDS             complement(2101..2952)
                     /gene="hchA"
                     /locus_tag="ACHY09_004703"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFS3887558.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(3544..>4612)
                     /locus_tag="ACHY09_004704"
     CDS             complement(3544..>4612)
                     /locus_tag="ACHY09_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFS3887559.1"
                     /translation="RKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIWSN
                     TDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFAGL
                     KYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSDFF
                     GLVDGLNFAVQYQGKNSNRDVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQAGD
                     GYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFDFG
                     LRPSLGYVYSKGKDLYARDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLDKD
                     DAAITDAATDDQFAVGIVYQF"
BASE COUNT         1286 a          932 c         1079 g         1315 t
ORIGIN      
        1 caggagtggg taacgcaggg gctttccgaa gcgggttatg tcattgatgc cgtttctgat
       61 ggcagagatg ggctttatct tgcgctgaag gatgattatg cattgatcat tctggatatt
      121 atgcttccgg gtatggatgg ctggcagatc ttacaaacgt taagaacggc aaagcaaact
      181 cctgttattt gccttacagc aagggattct gtcgatgaca gagtcagagg gctggacagt
      241 ggagcaaatg attatctggt aaaacctttt tcattttctg agttgctggc aagggttcgg
      301 gcacaattaa ggcaacatca cgctttgaat tcaacattag aaatcagtgg cttaagaatg
      361 gactctgtta gtcaaagtgt gagcaggaac aatatcagta ttacactgac gcgcaaggag
      421 tttcagttac tttggctact ggcctccaga gctggcgaaa ttatacccag aacggttatt
      481 gcgagtgaaa tttggggaat caactttgat agtgatacca atacggtgga tgtcgccatt
      541 cgcaggctcc gcgcaaaagt tgatgatcct tttcctgaaa agctcattgc cacaatccgt
      601 gggatgggat attcattcgt agcggtaaaa aaataatgaa aagattatct ataaccgtcc
      661 gtttaacctt gctttttata ttggtgctgt ctgttgctgg cgctggaatt gtatggactc
      721 tctataatgg cctggcaagt gagttgaaat ggcgcgatga tacaacactc attaaccgga
      781 cagcgcagat caagcagttg ttaattgatg gggtaaatcc agatacgtta cctgtctatt
      841 ttaaccggat gatggatgtt agtcaggata tcttgatcat tcatggtcat ggcatcaata
      901 aaattgttaa ccggacaaat gtcagtgatg gcatgttaaa taacatacct gctagtgaga
      961 caatcagcgc agctggcatt tacagaagca ttattaatga cacagagata gatgctttac
     1021 gaattaatat tgatgaagtt tcgccatcat taacggttac tgtggctaaa ttggcttcag
     1081 ccagacataa catgcttgaa cagtataaaa ttaatagcat tataatttgc attgtcgcca
     1141 ttgtactttg ctcagtatta agtccgctgt taatcagaac ggggttacga gagatcaaaa
     1201 agttgagtgg tgtaacggaa gcgctgaatt ataacgataa ccgggagcct gttgaggtta
     1261 gcgcattacc gagagaacta aaacctcttg ggcaggcgtt gaataaaatg catcatgctt
     1321 tagtcaaaga ttttgagcgt ctaagtcagt ttgctgacga tctcgctcat gaacttagaa
     1381 caccaattaa tgcattactg ggtcagaatc aggttacgct cagtcaaacc agaagtatcg
     1441 ctgaatatca aaaaacaatt gccggaaaca ttgaagagct ggagaatatt tcgcggttaa
     1501 cagagaacat actgtttctt gccagggcag ataaaaacaa tgttttggtg aaactggatt
     1561 cgctttctct caataaggaa gtcgaaaatt tgttggacta tcttgaatac ctttcagacg
     1621 agaaagagat ttgccttaag gtcgagtgca atcagcaaat ctttgcggat aaaattttac
     1681 tgcaacgaat gttatcgaat cttattgtta acgctattcg atattcacca gaaaaatcgc
     1741 gcattcagat aaccagtttt cttgatacca acggctctct taatattgat atcgccagtc
     1801 ctggaacgaa aattcatgag cctgaaaaac tcttccgtag attttggcgg ggagataatt
     1861 cgcgtcattc cgtaggtcag ggacttggtc tttctttagt caaagcgatt gccgaattgc
     1921 atgggggaag tgctacgtat cactatctca ataagcataa cgtgttccga attacgttac
     1981 cgcaaagaaa ttaatatagg gcttcagtac gccaaaatta accgtgaaaa aacgcagtca
     2041 cgtcgtgagt attaacgtgg ctgcgattga taatgcgctt acattacaat gtaacaggga
     2101 ttaacccgcg taagctgcca gcatttcctg cgccgccagt ttacccaacg cattcgctgc
     2161 gaaaggactg tcgccggtga gaactttacg gtccttatgt actcgcccgg tgatgtcgtc
     2221 attaatgatg ttcatgccca ttttcttcag ctcttcgccg aagtaccagg tgagatgacc
     2281 cggcatatag cctatctctg gcgtttgttt gtctgcggcg tctgggaatg cgcaaatgga
     2341 ataaccattc agtgggttat cgccgtggcg aagcgccaga aaagccgccg ggccgtggca
     2401 aagggagata acaaaacggt catttttgat tgcccactgt aaagcggcag ctacgtcctg
     2461 gctttcaggt aaaccaataa gtgcgccatg accaccagga acaaagattg ctgcatattc
     2521 gctatcagcg ttgaggcttg cgacaacatc cgcgagtttc ttcggattgc ggaacaacga
     2581 tttatgctgc tcaaagaatg gcattacttt ttcatctttg tgtggcatag cccagtattc
     2641 aaacttggtc atcagaccgg aaatggtcgc cacttcgaat tcgaaacctg cagcatggag
     2701 atgatacaac ggcagcaacg tttcaatcgg atggttaccg gtcgagaata gtttcccgtt
     2761 atcggtcggc aaataacgtt cgtccgcggc gatcaccaga attttatgtt taccgcgata
     2821 cggttttgga tagtccacgc catcaagatc agagacggga ctggtatatt ggctaagcga
     2881 atattctgaa gggaagaatg cattatcttc agcaatatcg acctgcgggt ttttacttgt
     2941 ttgaacagtc atagtgtatt ccttattgtt gcttagttag ggtagtcact atatttgcga
     3001 ctgagctggt cgctattttt aaccaggctg aaaaattgag tcaatatatc gaaagcggta
     3061 gttggcgggc ataagtatgt aatgatgatg ctgcactggt gttgaatatt attcacgcca
     3121 aattttcctt attgagctac gtcgtcgcaa gtggcctcaa tgattttaga ccattatcag
     3181 aggtccgggg cggcagggag agatttagtg ctgtggaata ttgtggtgct gatgaatatt
     3241 tatatcgcgg attggtagag aaaaagcttt tttcatgcac tatttttgca aatatttcta
     3301 tatggacggc ggttttgttg cggattaatt cataaagaaa tgcatttgga tgttggccgg
     3361 aagagacgct ggtagatttt attgagccag gaggggtggg gtaaggtgct gtatcgatat
     3421 gctttggcaa ttgcgtgtgc cgcagatgga gatgaaatgc caataaaaaa gcctgagttc
     3481 gatgagggac gaactcaggc tggtgttaag ttttacagta acacgccgcg gcgcgttggc
     3541 tgattagaac tggtagacga taccaactgc aaactggtca tcagttgcgg catcggtaat
     3601 cgctgcatcg tctttatcca gcaggttaaa tttgtatgct gtgtagacgt tcatgttctt
     3661 attgaagtag taccaggtac caacttcgat ataatttacg cggtcagcat caacaccttt
     3721 atgtccatca cgagcataaa gatctttgcc tttggaatat acgtagccta aagacggacg
     3781 taaaccgaag tcgaactgat actgtacaac agcttcaaag ttttgggttt tatttgcaaa
     3841 atgatcatta gccaaaacgg tcatgttgcg ggtttcaccg tacatcattg ctgcatagat
     3901 attatttgca tcatacttgg ctgctaatga ccacacttca gcgttatcac cgtagccgtc
     3961 accagcttgt tcattagtac gatcagattt gctatatgca cctacaaaac caaaaccttc
     4021 gatgttgtag tctacagaca acgcatagcc atcaccgttt tgtttcgtaa cgtcacgatt
     4081 gctgttctta ccttgatatt gcacagcaaa attcagacca tcaaccagac caaagaaatc
     4141 agagttacgg taggttgcaa caccgttggt acgaccgttc atgaagttgt cagcggacgc
     4201 ccaggaatca ccaccccatt caactaacat atcggtataa gctgcggcat cataagcaac
     4261 accgtagtta cgaccatagt cgaaagatcc taactcgtta tatttcaaac ctgcgaaggt
     4321 taaacgggtt ttttcctgat tagagccttc gggcttgctg gcgtcgaggt tgtattcaaa
     4381 ctgaccaaaa ccagtcagtt cgctggtgat ttgggtttca cctttaacac cgaaacgagc
     4441 ataggaggta tcttcgtttt cgctgttatt atcatcagta tttgaccaga tgcgttcgcc
     4501 aaccatcttg ccgtagaaat caactttatt gccatcttta ttataaattt cagccgcatt
     4561 tgctgcgcca gcaactaata acgccgggac cagcattgcc agaacttttc tt
//
LOCUS       DAZUCC010000289         4605 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZUCC010000289 DAZUCC010000000
VERSION     DAZUCC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.291"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..737)
                     /locus_tag="ACHY09_004705"
     CDS             complement(<1..737)
                     /locus_tag="ACHY09_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS3887560.1"
                     /translation="MLKDGAGKPVPGCTIELKARRTTETVIVTTVAYGQPGETGSYSM
                     DVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAV
                     AAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGE
                     TGPQGLQGVKGEQGEKGEKGEPGGPDATTAQKGIVQLSSTTDSDDETKAATPKAVKAV
                     MDKADGCLEKAKNGEDIPDKVQFLNTVG"
     gene            complement(901..1041)
                     /locus_tag="ACHY09_004706"
     CDS             complement(901..1041)
                     /locus_tag="ACHY09_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3887561.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHSSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            complement(1241..>4605)
                     /locus_tag="ACHY09_004707"
     CDS             complement(1241..>4605)
                     /locus_tag="ACHY09_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3887562.1"
                     /translation="EGPVNGLQSILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPE
                     GFESSGAETALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVETTSKGDRNPASVRL
                     LIQLERNGHWVTEKDITINGKTTSQYLTSVILNNLPPRPFNIRMVRETADSTTDQLQN
                     RTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMAVNYHIRGRIIQVPSNYDPEK
                     RTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQS
                     VPDGFGGTEPRMTFNAYLSQQRKAWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVW
                     PYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYG
                     RNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDND
                     YAGTMTGGRILSIDSASRTLTLDREVTLPDTGTSTVNLINDSGKPVRVDITAHPAPDR
                     IQVSALPDGVEAYGVWGLSLPSLRRRLFRCVSIRENTDGTFAVTAVQHVPEKEAIVDN
                     GATFEPQSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGS
                     GENSRLVTSAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAAPDS
                     IELTPGYFQITAVPRLAVYDPTVQFEFWFSEKRITDMAQVETSARYLGTGSQWSVSGP
                     HIKPGTDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLELFREKIGKLHLAQGMWELI
                     DNSQLDDEMAEMKTTITETRNEITQTVNKTLEDQSATIQQIQRVQTDTNDDLAALYML
                     KVQKTKDGIPYVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVAQGNQL
                     FMNDVFLKRLFAVSITSSGNPPTFSLTPDGRLTARNADISGHISANSGTLNNVVIAEN
                     CTINGTLRAEKITGDLVKCAGVAFPVDGSHLANGTRTLTVYDDHSFDRQIIIPPIIYV
                     GSKQESRTSNDIWTECFLHVDQNGRRIYSGRSVTEPGVFSGIIDMPAGGGHITLSFTV
                     SSRRQNGSFGSSRISNLQAIVVKKNSTGISIR"
BASE COUNT          847 a         1493 c         1165 g         1100 t
ORIGIN      
        1 cccacggtgt tcagaaactg caccttatcc gggatatctt caccgttttt cgctttctcc
       61 aggcatccgt cagctttgtc cattaccgct ttcaccgcct tcggggttgc tgccttcgtt
      121 tcatcatcac tgtccgttgt gctgcttaac tgcacaattc ccttctgtgc cgtcgtcgca
      181 tccggccctc ccggctcgcc tttttcgccc ttctctccct gttcaccttt cacaccctga
      241 agcccctgtg gccctgtctc accacgctca ccctttggcc cacgctcgcc ggtatcgcct
      301 tttggcccgg gaataccctg cggcccggtg tcccccttat cccccttcgg tccccgcgca
      361 ttctctgccc gtttcttcgc ttcctctgca ctcgccgctg acgcttccgc acgcttcaga
      421 atttccgctg ccaccgcttc cagctctgca agcgctttcg ggtaatactg tgcgtcctcc
      481 aggtccatca gaaatttatt cagcgttccc ggtgcagaat ccgccttcac cagaatgtca
      541 cccacatatg acggcgcgta accttccgtg ttcagcgtca cacggtacag acccggctca
      601 acatccatac tgtaactgcc ggtttccccc ggctgaccat acgccaccgt ggtgacaatc
      661 accgtctccg ttgtgcgccg cgctttcagc tctatcgtgc atcccggtac cggcttcccg
      721 gcaccgtcct tcagcacccc cgatattctt actgccatgg atttccccca taaaaaaacc
      781 gcagtaccgg tttcccggcc tgcggtaaaa tttgtggttt gttggtgtta aaacggtgcc
      841 atccggctga ccaccctcag caaccggtcg gcgggggata ttctcccccg ccacggtttc
      901 ttactgctta cactgtaaga acgccgcaat ctccgcgccc gctatccgga accggaactc
      961 gcacagtgaa ctgtgggtga tccagataat gagcactacc gtgatacaaa tcacggtggt
     1021 ttttaacggt ttttgcgaca taaacgcttg ctccttttac ggagaggcgc taacctttca
     1081 cttgcttagg atgaatggtc aggcctcggg ttaaacatga atgttttgtc cggggccttt
     1141 tcacatccgg ccttcaggtg ttccctccgg ccatcagcca aaggcacccg cgcataatgt
     1201 acggttttta tctccttccg gcaatcccgg ggcactacgt tcagcgaata ctgatccccg
     1261 tgctgttttt cttcaccact atcgcctgaa ggttactgat acgtgaactg ccaaagctcc
     1321 cgttctgacg ccgtgaactc acggtaaaac tcagggtgat atgaccacca ccggcgggca
     1381 tatcaatgat cccgctgaaa actcccggct ctgtcactga cctgcctgaa taaatccggc
     1441 gtccgttctg atcaacatgc aggaagcact ctgtccagat gtcattactg gtgcgggatt
     1501 cctgttttga cccgacatag attatcggcg gaattataat ctgccggtca aagctgtgat
     1561 cgtcatacac cgtcagcgtg cgtgtaccgt tcgcaagatg actaccatcc accggaaaag
     1621 ccacacctgc acatttaaca agatcaccag taattttctc cgctctcagc gtgccattta
     1681 tcgtgcagtt ctccgctatc acgacattat tcagcgtgcc cgagttcgca ctgatatgtc
     1741 cgctgatgtc cgcattacgg gccgtcagcc tgccgtccgg cgtcagggag aacgtcgggg
     1801 gattgccgga cgaggtgatg ctcaccgcaa acagtcgctt caggaacacg tcgttcatga
     1861 acaactgatt cccctgcgcc acaaacagcg gcgtggtgtt gccgttctcc gggttaatca
     1921 tcgcgatacg gtccgcctgc agcagaatat tgctcagggg ctggccatct gcatcctcaa
     1981 tccccgcacc aataccggca acatacggaa tgccatcctt tgttttctgc accttcagca
     2041 tgtacagcgc agccaggtca tcatttgtgt ctgtctgcac ccgctgtatc tgctgtatgg
     2101 tggcgctctg gtcttccagg gttttgttga ccgtctgcgt gatttcattg cgggtttccg
     2161 tgatggtggt cttcatctcc gccatctcat catcaagctg gctgttatca atcagctccc
     2221 acatcccctg tgccagatgc agttttccta tcttttcccg gaacagttcc agataacctt
     2281 ccgcatcatt gctggcccgt ccgctggctt ccacaaaagc agatttcccc accaggttga
     2341 cgctgcgcac ataaaaccag aaatccgtcc ccggcttaat gtgcgggccg gagacgctcc
     2401 actggctgcc tgtccccaga taacgggcgg acgtttccac ctgtgccatg tccgtgatgc
     2461 gtttttccga aaaccagaac tcaaactgta ccgtcgggtc atacaccgca aggcgcggta
     2521 ccgccgttat ctgaaaatac cccggcgtca gttcaatgct gtccggtgct gccggtgcgt
     2581 taatccggaa cgtggtggtg gcaggttcgc cctgctggcc gtagctgttt atcgccctga
     2641 ccgtcagggt gtattccccc agcggcaggc cactgaaacg gtgctccgtg tcggcagtga
     2701 tggcactggt caccagacgg ctgttttcac cgcttccact ggtcaggcgc aggctgaagc
     2761 gcacgccctt caccacccgc ggcgtgtccc atttcgccag cgccagatac tggccatctg
     2821 aggcactcac ctccaccgta agatgctgca ctgccggagg aatgacgctg ttcagggagc
     2881 ctgactgcgg ctcaaaggtg gcaccgttat ccacaatggc ctctttttcc ggcacgtgct
     2941 gcactgccgt gacggcaaag gtaccgtccg tgttttcccg gatggaaaca cagcggaaca
     3001 ggcgacggcg cagtgacggc agggagagtc cccacactcc gtatgcctcc acgccatccg
     3061 gcagggcgct gacctgtatc cggtccggcg cggggtgtgc agtgatgtcc acgcgtaccg
     3121 gcttaccgct gtcgttaatc aggttcaccg tggatgtgcc ggtatccggc agggtcacct
     3181 cacggtccag cgtcagggta cggctggcgc tgtcgatgga cagaatacgc ccgccggtca
     3241 tggtcccggc gtagtcgtta tcacagattt caatgatgtc accgggcgtg tgccgcagcc
     3301 cctgtgaccc gagcgtgaaa tccaccgtct gtgtttccag cagttcggtc tttatcaccc
     3361 acagtccggc ccggtgagcc tgaccgcggc tggtacagcc aaacgcatcc atcttcagca
     3421 ggttgcgtcc gtagcgcagt atggcctccg ggtcttccac cagttccgtg gaagtctgcc
     3481 agccgttctg cgggtcggtg taattcacct ccaccgccgt gtgccggtcc ttcagggcac
     3541 tgaagctgta gcggaacccc acgccgttat catccaccac cacatcgctg ttggtgtagg
     3601 gccacacaac atccgacgga cggtcctgaa cgaacgtcag cgtctggccg ttccataccg
     3661 gcatacagcg catcgccgag cagaaatccc ccaggacatc ccacgcctta cgctgttgtg
     3721 acaggtacgc attaaaggtc atccgcggct cagttccccc gaagccgtcc gggacactct
     3781 ggtcgcagta ctgcccgatg gcatacagcg cccatttgtc cacgtccgcc gcccccagac
     3841 gttttcccat cccgtagcgc gggtgggtca gcatgtccca caggcaccag gccgggttgt
     3901 tgctgtatgc cggtttcaga ctcccgtccc agataccgct gtacgtgcgt ttttccgggt
     3961 catagtttga cggcacctga atgatgcgac cgcggatatg gtagttcacc gccatctgct
     4021 gaccaccaaa ctgctccgca tccacctgca ggcccacaat ggccgtgttc gggtagcact
     4081 gtttcacatc gatgatttca gtgtatgacg accacagcgt tctgttctgc agctggtccg
     4141 tggtgctgtc cgccgtttcc ctgaccatcc ggatgttaaa ggggcgggga ggcagattat
     4201 tcagaatcac cgacgtcagg tactgcgagg tggtcttgcc gttaatggtg atatcctttt
     4261 ctgtcaccca gtgtccgtta cgctcaagct gaatcagcag ccggacagaa gccggattac
     4321 ggtcaccctt tgaggtggtc tccaccagtg actgcacccc gaaggtgacc cgcagacggt
     4381 caatgttcgc tgacgtgatg gtgcgcgtca ccggctttgc cttcgtcacc tccacgccca
     4441 gtgcggtttc agccccggag gactcaaaac cttccggcgg cgtctgctcc tgctccccgg
     4501 cacgccagac ggcggtcaca ccgtgtatca cgggattacc gtccgtgtcc gtcagcgggg
     4561 ttttgttcac cagaatactc tgcaggccgt taaccggacc ttcca
//
LOCUS       DAZUCC010000290         4603 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZUCC010000290 DAZUCC010000000
VERSION     DAZUCC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.292"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..593)
                     /locus_tag="ACHY09_004708"
     CDS             complement(<1..593)
                     /locus_tag="ACHY09_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS3887563.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(708..783)
                     /locus_tag="ACHY09_004709"
     tRNA            complement(708..783)
                     /locus_tag="ACHY09_004709"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Thr,seq:ggt)
     gene            complement(790..864)
                     /locus_tag="ACHY09_004710"
     tRNA            complement(790..864)
                     /locus_tag="ACHY09_004710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Gly,seq:tcc)
     gene            complement(981..1065)
                     /locus_tag="ACHY09_004711"
     tRNA            complement(981..1065)
                     /locus_tag="ACHY09_004711"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1029..1031),aa:Tyr,seq:gta)
     gene            complement(1074..1149)
                     /locus_tag="ACHY09_004712"
     tRNA            complement(1074..1149)
                     /locus_tag="ACHY09_004712"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Thr,seq:tgt)
     gene            1511..2461
                     /gene="coaA"
                     /locus_tag="ACHY09_004713"
     CDS             1511..2461
                     /gene="coaA"
                     /locus_tag="ACHY09_004713"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS3887564.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHY09_004714"
     CDS             complement(2490..3455)
                     /gene="birA"
                     /locus_tag="ACHY09_004714"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFS3887565.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHY09_004715"
     CDS             complement(3452..4480)
                     /gene="murB"
                     /locus_tag="ACHY09_004715"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS3887566.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQSVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1181 a         1157 c         1046 g         1219 t
ORIGIN      
        1 gaatccagga agccagccag ttccaggatt ttcgcttccc actctgcgtc gccttccagc
       61 gctttcagag cagaaccacg aacgatcgga gtgtcgtcgc ccgggaagtc gtactgagac
      121 agaagttcac gaacttccat ttcaaccagt tccagcagct cttcgtcatc aaccatgtcg
      181 catttgttca ggaacacgat gatgtacgga acgcctacct gacgccccag caggatgtgc
      241 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
      301 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgc
      361 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      421 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      481 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      541 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      601 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      661 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      721 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      781 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      841 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      901 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      961 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
     1021 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
     1081 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
     1141 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
     1201 ttgcataatt tgttttattg gtcacatttt atgcgacacg atgaagaaac agccgaacgg
     1261 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgtgctc ctgctaaacc
     1321 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggg
     1381 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1441 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgcctg
     1501 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1561 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1621 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1681 tctgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1741 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggtagtgt
     1801 cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1861 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1921 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1981 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     2041 tcttatttat gatgtgatcc cggatggaga taaaactgtt gttcagcctg atattttaat
     2101 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     2161 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     2221 gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttacag acccggattc
     2281 ctattttcat aactacgcga aattaactaa agaagaagct attaagactg ccatgacatt
     2341 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gcgagcgtgc
     2401 cagtttaatc ctgacgaaaa gtgctaatca tgcagtagaa gaggtcagac tacgcaaata
     2461 atttaaaggg gagttaatgc tcccctttct tatttttctg cactacgcag ggatatttca
     2521 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcacc ctgtttgtct
     2581 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2641 ataaaattat ccaacttttc ccagcgcgaa agataaggtg tcaatccttc ttgctcgaag
     2701 agttccagcg ccgcgcgtaa ttcacgtatc agcatggccg caagagtatt acgatcgaga
     2761 ttgatcccgg cttcctgcag cgtgatccac ccttgattaa cgacactctc ttcaacacga
     2821 cgcattgcca tgttgatccc tgcaccaatg actatttgcg ccgcatcgcc ggttttgcca
     2881 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2941 ttaacacgaa ctttatctgc acccagcttg cgtaatactt ccgccatcac gataccgata
     3001 accagactta aaccaatcgc cgccgccggg ccctgttcca gacgccagaa catcgacaaa
     3061 tataagttcg cgccaaaagg cgaaaaccat ttccgaccac gacgaccacg gccagcatgc
     3121 tggtattctg caacacaggc atcgccagat ttaagctctc cgatgcggtc caggagatac
     3181 tgattcgtgg agtcaataac tggcagcacg gctacactgc cgtcatccag ctgactcaat
     3241 atcttttcgg cattaagtaa ctggattggc tcgggcaagc tgtatccttt acccggaaca
     3301 gtaaatacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3361 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcgccatt cgctaacagg
     3421 gcgatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3481 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3541 ctttttgtct tacatgatgc gccagctgta caacatcttc gctttttgca tcgtgctcat
     3601 ttatgagaac cagcgcctgt tgacggtgta ccgcagcccc accaatttgc gtccctttaa
     3661 gctggcactg atcaataagc caacctgcag ccagttttac tgaaccatcc acctgggggt
     3721 aatgtggggc tgttggaaat tgtgccaaca acgcgttagc agtttcggca gaaacaacag
     3781 gatttttgaa gaaactaccc gcattaccgt tcacttttgg atcagggagt ttggtggtgc
     3841 gcatatgaca caccgcatca aatacttgct gtggcgttac cgttgaagga tccaggcgag
     3901 ttaagtcacc atacgtcagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3961 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtctcgatag ccaaaacggc
     4021 actctttggc agttaagcgt acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     4081 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     4141 tacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     4201 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     4261 catgaatttc gatacctttg atccggttga tgatcaccgt accgcgatag tcttccagaa
     4321 aaagtacatt acttccttca cccagaataa gaacggattg tccttttgcg gttgcatgct
     4381 gccaggcatt gagtagttgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4441 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4501 aacgaggcta gtttaccgta tctgcggggg gatggcttgt agatatgatg acaggaagag
     4561 tttgtagaaa cgcaaaaagg cccagtcttc cgactgggcc ttc
//
LOCUS       DAZUCC010000291         4570 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZUCC010000291 DAZUCC010000000
VERSION     DAZUCC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.293"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(227..1279)
                     /locus_tag="ACHY09_004716"
     CDS             complement(227..1279)
                     /locus_tag="ACHY09_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFS3887567.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKSASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPKYELPLSKDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIEKIQSETDIPIVIVHGPKGEEKARVLVDCYNNVFRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLLRI"
     gene            1594..2910
                     /gene="shiA"
                     /locus_tag="ACHY09_004717"
     CDS             1594..2910
                     /gene="shiA"
                     /locus_tag="ACHY09_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFS3887568.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            3012..4466
                     /gene="amn"
                     /locus_tag="ACHY09_004718"
     CDS             3012..4466
                     /gene="amn"
                     /locus_tag="ACHY09_004718"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFS3887569.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         1118 a         1086 c         1036 g         1330 t
ORIGIN      
        1 ggggcgctct tttttacatt ctctctacag aacttgccct tctctcacag aaaatgaata
       61 aataaaaatg cgataccgcc agattcgcgt tcggtgtgac tatggttgta tccggtgagt
      121 acagctttga tccaaaggct ggctttctgt aatgattgac cagccacaaa gtattgatga
      181 caccaccgtt cataacagat cggcactact atgcataagt actgcctcat attctcaaca
      241 aaacactttt taatgccttt gcgaattcat ccagacttat attgtcaatt ttttgcccaa
      301 cgacgactga ctccgagaca tcggccattg cgggccatcg cgttttgtaa tcagcataaa
      361 ttgcaacaac gggggtatta taggcacttg ccatatgtaa tattgaggtg tcaggagtta
      421 ttgcgatata agcatctttt atgattgctg ccgaacgttt aatcgagggt gataaggata
      481 aacggaagac attattataa caatccacta acaccctggc tttttcttcg ccttttggtc
      541 catgaacgat cactattggt atatctgttt cactttgaat tttttctatt agattttctg
      601 caatcgataa tgaaaatgta cgttcctgcg agctaccttc taaattaagc gcaatgtaag
      661 atcctaacga tcgcatttcc tcgcgcacct catcgagtac atccttactt agtggcaatt
      721 catattttgg cctgactgct ggaaaacccg cctcacgcat cataaatgcc catgtcatgg
      781 gtacaggagc ccggagattc tgatccatcc gggacgcgct cttacacaaa ggggaatagc
      841 acttcatggt taaaccaaca acctgaaaat tcgtttttgc tttcaactga ctgataaata
      901 tcatcgtttt caggttcttt ttacgcatcg cctctataca aagatccggc gtaccgtatt
      961 gctgtgttat gttcttcgct agatctttta tttcttttaa tgttgcgtga tcctgcatag
     1021 tcattgtaac taatgttaat ttagtctgtt caagtttgag cgcattaaag acttctaaat
     1081 taattgtcga cgttacaatt aaaagatgct taattttatg caattcaagc gcccgaataa
     1141 caggaaagat ggccatagca tcgccaatct gatcgggaat atggatgaca acaaagtctg
     1201 ttttttcaat attgaaatta taagctttat aatcgtagta actaaatgca atacgtctca
     1261 acaatgatgc taaaaacata cctaacctcg cctccctact ggttataatg caatgcagtc
     1321 tatcagactc atcagggtgc cattttgtgc atatgcggac ttttatgttt catatctcga
     1381 acctgtgagg cctctgctta atccttaaac aacaccagca actcctgcgc tttcatcttc
     1441 cagcgaattt ttcatgttgc tgctcatcag ccataaaatc atttgcagat gcgctctgtc
     1501 gaggtagtct cataaggttc gtttatagat cgacggcaat gtgagttacc ttttccatac
     1561 taattataaa aagacagtac aaacaggatc attatggact ccacgctcat ctccactcgt
     1621 cccgatgaag ggacgctttc gttaagtcgc gcccgacgag ctgcgttagg cagtttcgct
     1681 ggtgccgtcg tcgactggta tgatttttta ctctatggca tcaccgccgc actggtgttt
     1741 aatcgcgagt ttttcccgca agtaagcccg gctatgggaa cgctcgccgc atttgccacc
     1801 tttggcgtcg gattcctgtt ccgtccgctc ggcggtgtca ttttcggtca ctttggcgac
     1861 cgactgggac gtaagcgcat gttaatgctg accgtctgga tgatgggcat cgcgacagcc
     1921 ttgattggta ttcttccttc attctcgacc attgggtggt gggcacctat tttgctggtg
     1981 acactgcgtg ccattcaggg atttgccgtc ggcggcgaat ggggaggcgc ggcgttgctt
     2041 tccgttgaaa gtgcgccgaa aaataaaaaa gccttttaca gtagcggtgt acaagttggc
     2101 tacggtgtag gtttactgct ttcaaccgga ctggtttcat tgatcagtat gatgacaact
     2161 gacgaacagt ttttaagctg gggctggcgc attcctttcc tgtttagcat cgtactggta
     2221 ctgggagcat tgtgggtgcg caatggcatg gaggagtccg cggaatttga acaacagcaa
     2281 cataatcaag cggccgcgaa aaaacgcatc ccggttatcg aagctctgtt acgacatccc
     2341 ggtgctttcc tgaagattat tgcactacga ctgtgcgagt tgctgacgat gtacattgtt
     2401 actgcctttg cacttaatta ttcaacccag aatatggggt taccgcgcga acttttcctt
     2461 aatattggtt tgctggtagg tggattaagc tgcctgacaa ttccctgttt tgcctggctt
     2521 gccgatcgtt ttgggcggcg cagggtttat atcacaggtg cgttaatcgg aacgttgagc
     2581 gcatttcctt tctttatggc gcttgaagca caatctattt tctggatagt tttcttctcc
     2641 atcatgctgg caaacattgc gcatgatatg gtggtgtgtg tacaacaacc gatgtttacc
     2701 gaaatgtttg gtgccagtta tcgctatagt ggcgctggag tcggttatca ggttgccagt
     2761 gtggttggcg gtggatttac accttttatt gccgctgcac tcatcactta ctttgccggg
     2821 aactggcata gcgtcgccat ttatttgctg gctggatgcc tgatttccgc aatgaccgct
     2881 ttgttgatga aagacaatca acgcgcttaa tagcctggcg aagatcatcc gatcttcgcc
     2941 ttacactttt gtttcacatt cctgtgacat actatcggat gtgcggtaat tgtatggaac
     3001 aggagacaca catgaataat aagggctccg gtctgacccc agctcaggca ctggataaac
     3061 tcgacgcgct gtatgagcaa tctgtagtcg ctttacgcaa cgccattggc aaatatatta
     3121 caagtggcga attacctgat gaaaacgccc gcaaacaagg tctttttgtc tatccatcac
     3181 tgaccgtaac ctgggacggt agcacaacca atccccccaa aacgcgcgca tttggtcgtt
     3241 ttacccacgc aggcagctac accaccacga ttactcgccc tactctcttt cgttcgtatc
     3301 tcaatgaaca acttacgttg ctgtatcagg attatggtgc gcatatctcg gtgcaaccct
     3361 cgcagcatga aatcccttat ccttatgtca tcgatggctc tgaattgaca cttgatcgct
     3421 caatgagcgc tgggttaact cgctacttcc cgacaacaga actggcgcaa attggcgatg
     3481 aaactgcaga cggcatttat catccaacgg aattctcccc gctatcgcat tttgatgcgc
     3541 gccgcgtcga tttttccctc gcgcggttgc gccattatac cggtacgcca gttgaacatt
     3601 ttcagccgtt cgtcttgttt accaactaca cacgttatgt ggatgaattc gttcgttggg
     3661 gatgcagcca gatcctcgac cctgatagtc cctacattgc cctttcttgt gctggcggga
     3721 actggatcac cgccgaaacc gaagcgccag aagaagccat ttccgacctt gcatggaaaa
     3781 aacaccagat gccagcatgg catttaatta ccgccgatgg tcagggtatt actttggtga
     3841 atattggcgt gggaccgtca aatgctaaaa ccatctgcga tcatctggca gtgctacgcc
     3901 cggatgtctg gttgatgatt ggtcactgtg gcggattacg tgaaagtcag gccattggcg
     3961 attatgtact tgcacacgct tatttacgcg atgaccacgt tcttgatgca gttctcccgc
     4021 ccgatattcc tattccgagc attgcggaag tgcaacgtgc gctttatgac gctaccaagt
     4081 tagtcagcgg caggcctggt gaagaagtca aacagcggtt acgtactggc acggtggtaa
     4141 ccacagatga caggaactgg gagttacgtt actcagcttc tgcacttcgt tttaacttaa
     4201 gccgggccgt agcaattgat atggaaagtg caaccattgc cgcgcaagga tatcgtttcc
     4261 gcgtgccata cgggacacta ctgtgtgttt cagataaacc gttacatggt gagattaaac
     4321 ttcccggcca ggctaaccgt ttttatgaag gcgctatttc cgaacacctg caaattggca
     4381 ttcgggcgat cgatttgctg cgcgcagaag gcgaccgact gcattcacgt aaattacgaa
     4441 cctttaatga gccgccgttc cgataataag ttatgaacct tcttatcaga aatagatgtg
     4501 aaggattaag taagacatct ggcaaatagc ctgcaataaa cgctggctgt ttgcttcctt
     4561 gggcagatgc
//
LOCUS       DAZUCC010000292         4549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZUCC010000292 DAZUCC010000000
VERSION     DAZUCC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.294"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            221..1378
                     /gene="acrE"
                     /locus_tag="ACHY09_004719"
     CDS             221..1378
                     /gene="acrE"
                     /locus_tag="ACHY09_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFS3887570.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1390..4494
                     /gene="acrF"
                     /locus_tag="ACHY09_004720"
     CDS             1390..4494
                     /gene="acrF"
                     /locus_tag="ACHY09_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFS3887571.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1110 a         1102 c         1193 g         1144 t
ORIGIN      
        1 acgaggattt gattctacct ctttaaaccc gcgtcaaaat aaaacagtag aatattaatc
       61 tttttttgtg tttatgtgcc ttgagacgcc agtattcata actattcctt acatcgacga
      121 atgataattt gtaggatagc gaactgtatt tttctttctg cgagttaacg cgttgccttt
      181 ttgggtaaat aacgcgcttt tggttttttg aggaatagta atgacgaaac atgccaggtt
      241 tttcctcctg ccctccttta ttctgatctc cgcggcttta atcgccggtt gtaacgataa
      301 gggagaagag aaagctcacg tcggtgaacc gcaggttacc gttcatattg tgaaaacggc
      361 cccgttagaa gttaagactg aattaccagg ccgcaccaat gcttaccgta tagccgaagt
      421 tcgcccacag gttagcggga tcgtactgaa tcgcaatttc actgaaggca gcgatgtgca
      481 agcaggccag tccctgtacc agatcgatcc cgcgacctat caggcaagtt atgacagcgc
      541 gaaaggcgaa ctagcgaaaa gtgaagccgc cgccgccatc gcgcatttga cggtaaaacg
      601 ttacgttccg ctcgtgggta cgaaatacat cagtcagcag gagtacgacc aggccattgc
      661 tgatgctcgc caggccgatg ccgccgtgat tgccgcaaaa gctacagttg aaagcgctcg
      721 catcaatctc gcttatacca aagtgactgc gcctattagc ggacgtatcg gcaaatcgac
      781 tgtgaccgaa ggcgctcttg tcactaatgg gcaaacgact gaactggcga ctgtccagca
      841 gctcgatcct atctacgttg atgtgaccca atccagcaac gattttatga ggctgaagca
      901 atccgtagag caaggaaatt tgcataagga aaacgccacc agcaacgtag agttggtcat
      961 ggagaacggt caaacctatc ccctgaaagg tacgctgcaa ttctccgatg tgaccgttga
     1021 tgaaagcacc ggctccataa ccctacgtgc tgtcttccct aacccgcaac atacgctttt
     1081 gccgggtatg tttgtgcgcg cacggattga tgaaggcgtc caacctgacg ccattcttat
     1141 cccgcaacaa ggcgttagcc gcacaccacg tggtgatgca accgtgctga ttgttaacaa
     1201 taaaagtcag gttgaagcgc gccctgtcgt tgccagccag gcgattggcg ataaatggtt
     1261 gattagcgaa gggctgaaat ctggcgatca agtcattgtc agcggcctac aaaaagcgcg
     1321 tccgggagag caggttaaag ccactaccga tacccccgca gatactgcat cgaagtaagg
     1381 caatctgaca tggcaaactt ttttattcga cgaccgatat ttgcatgggt gctggccatt
     1441 attctgatga tggcgggcgc actggcgatc ctacaattgc ccgtcgctca gtatccaacc
     1501 attgcaccgc ctgcggtttc tgtttcagca aactatccgg gcgctgatgc gcagaccgtg
     1561 caggatacgg tgacgcaggt tatcgaacag aatatgaacg gtatcgataa cctgatgtat
     1621 atgtcctcca ccagcgattc cgccggtagc gtgacaatta cccttacctt ccagtccggg
     1681 accgatcctg atatcgcgca agtgcaggtg cagaacaaac tccagctcgc cacgccgttg
     1741 ctgccgcagg aagtacagca gcaggggatc agtgttgaaa agtccagtag cagctatttg
     1801 atggtggcgg gctttgtctc tgataaccca ggcaccacac aggacgatat ctcggactat
     1861 gtggcctcta acgttaaaga tacgcttagc cgtctgaatg gcgtcggtga cgtacagctt
     1921 ttcggcgcac agtatgcgat gcgtatctgg ctggacgccg atctgctaaa caaatataaa
     1981 ctgacaccgg ttgatgtgat taaccagttg aaggtacaga acgatcagat cgctgccgga
     2041 cagttgggcg gaacgccagc gttaccaggg caacagttga acgcctcgat tattgctcag
     2101 acgcggttaa aaaatccgga agaattcggc aaagtgaccc tgcgcgtaaa cagtgacggc
     2161 tcggtggtac gcctgaaaga tgtcgcacgg gttgaacttg gcggtgaaaa ctacaacgtt
     2221 atcgctcgta tcaacggaaa accggcggcg ggcctgggga ttaagctggc aaccggcgca
     2281 aatgctctcg ataccgcgaa agccattaag gcaaaactgg cggaattaca gccattcttc
     2341 ccgcagggaa tgaaggttct ctacccttat gacaccacac cattcgtcca gctttctatt
     2401 cacgaagtgg taaaaacgct gttcgaagcc attatgctgg tgttcctggt gatgtatctg
     2461 ttcttgcaga atatgcgagc aacgctgatc cccaccattg cggtacccgt ggtgttgtta
     2521 gggacgtttg ccatcctcgc cgcttttggt tactccatca acacactaac gatgttcggg
     2581 atggtgcttg ccatcgggct gctcgtcgat gatgcgatag tggtggtgga gaacgtcgag
     2641 cgcgtgatga tggaggataa gctcccgcca aaagaagcga cggaaaaatc aatgtcgcaa
     2701 atccagggcg cactggtggg tatcgcaatg gtgctgtcag cggtatttat tccgatggca
     2761 ttcttcggcg gttctactgg ggcaatttat cgtcagttct ctatcaccat cgtttcggca
     2821 atggcgcttt ctgttctggt ggcattgatt cttacccctg cgttatgtgc aacgctgctt
     2881 aaacccgtct ctgctgagca tcacgaaaat aagggcggtt tcttcggctg gtttaatacc
     2941 accttcgatc atagcgttaa ccactacacc aacagcgtcg gcaaaatcct cggttccaca
     3001 ggacgatatt tactgatcta tgcactaatt gttgcaggaa tggtggtgtt gtttttacgt
     3061 cttccgtctt ccttcttacc tgaagaggat cagggtgtct ttctgactat gattcagtta
     3121 cccgctggcg cgacgcaaga gcggacgcaa aaagtactgg atcaagtgac ggattactat
     3181 ctgaagaacg agaaagcgaa cgttgaaagt gtctttacag ttaacggctt tagcttcagc
     3241 ggccaggcac aaaacgccgg tatggctttc gtcagtctga aaccgtggga agagcgtagt
     3301 ggtgacgaaa acagtgcgga agcggtaatc catcgtgcca aaatggaatt gggcaagatc
     3361 cgcgacggtt ttgtcattcc attcaatatg ccagccattg ttgaactggg cacggcaacg
     3421 ggtttcgact ttgagttaat tgatcaggct gggctgggtc atgatgccct gacccaggcc
     3481 cgtaaccagt tgcttggcat ggcggcgcaa catcctgcca gcttagtcag cgtgcgtcct
     3541 aatggtctgg aagacaccgc gcagtttaaa ctggaagttg accaagaaaa ggcgcaggca
     3601 ttaggtgttt cactttctga catcaatcag accatttcaa cagcgttggg tgggacttac
     3661 gttaacgact tcattgaccg tggtcgcgta aaaaaggtgt atgttcaggc ggatgccaaa
     3721 tttcgtatgc tgccagaaga tgtcgataaa ctttatgtcc gtagcgccaa tggcgaaatg
     3781 gtgccattct ctgcctttac cactgcacat tgggtgtatg gttctccgcg actggagcgc
     3841 tacaacggtc tgccgtcaat ggagattcag ggggaagccg cgccaggaac cagttccggc
     3901 gatgccatgg cgttgatgga aaaccttgca tcacaattac cagctggtat tggttatgac
     3961 tggacgggta tgtcatatca ggaacgctta tcgggaaacc aggctcccgc tctggtagca
     4021 atttcctttg tggttgtttt cctgtgcctt gctgcactct atgaaagctg gtcaattcct
     4081 gtctcggtta tgttggtcgt gccgttaggg attgtcggcg tgctactggc ggcgacactc
     4141 tttaatcaaa aaaatgacgt ctactttatg gtgggcttgc taacgacaat tggcttgtcg
     4201 gctaaaaacg ctatattgat tgttgagttc gctaaagatc tcatggagaa agagggtaaa
     4261 ggtgttgttg aagcgacact aatggcagta cgtatgcgtc tgcgtcctat cctgatgacc
     4321 tctctcgcct ttattctcgg cgtattaccg ctagctatca gtaacggtgc cggcagtggc
     4381 gcgcagaacg cagtgggtat cggggtaatg ggcgggatgg tttctgcaac gttgctggca
     4441 atcttcttcg tgccggtgtt ctttgtggtg atccgccgtt gctttaaagg ataaatcaga
     4501 aacataaagg cactttcggg tgcctttatt atttccagtg aaacccata
//
LOCUS       DAZUCC010000293         4541 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZUCC010000293 DAZUCC010000000
VERSION     DAZUCC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.295"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..998
                     /locus_tag="ACHY09_004721"
     CDS             <1..998
                     /locus_tag="ACHY09_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HFS3887572.1"
                     /translation="GNLSFRSWLARVTQMRLKLQQVTSAPDPQAMSQMLARAVFQGKA
                     IDLTDTCDYGSLVAASLGQEWSGFGQSLFVQPLDLAWRQVLVPAAGSLNARWQTTIVD
                     QWNKAFAGRYPFKATGSDASLPLLAQFLRSDSGRITTFLKTHLGGLVHQEGNHWVAEP
                     SASQGMVVSPDFLRAINQLAELSDIVFSQGDAGVHFELMARPSRDVARVQLTLDEQNL
                     DYFNQMESWQSFTWPGNTYYPGVSLSWRSVSSGMQLYASHQGNWGFIRLLDEALITPL
                     DASRTQLVWITADGNPLKFVMRSELGDGPLALLKLQGFTLPTAIFNVAAGTEGVE"
     gene            1120..1287
                     /locus_tag="ACHY09_004722"
     CDS             1120..1287
                     /locus_tag="ACHY09_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887573.1"
                     /translation="MSEKTTDKLTEGQTHSAGERMLLKWRPPQIFNSKDDASPEAVVR
                     FMKETDKQEKE"
     gene            1284..2897
                     /gene="tssA"
                     /locus_tag="ACHY09_004723"
     CDS             1284..2897
                     /gene="tssA"
                     /locus_tag="ACHY09_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFS3887574.1"
                     /translation="MSSPEALLTACAASQEERLSLISRANEALSLWDNWLHPFTSGSE
                     TGDDPAYDDNFQLMREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLTRD
                     GERGLSEGLLLLVAMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGVDSNEA
                     GLTAGAISLLGSAVADWPEAEKPSFAGLCSALENRLARSGGMEALVPQNSSPRETGRE
                     SAPPDSPQLAAVTSGRDLLDQARLLSRWLSGQPQGWLASHRLMKTVRWDTVDQLPSLD
                     SSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLWQGLS
                     HAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEEDVMY
                     GDEAAIPAVVNGQTDDVLILESEAMEKGDTEGPEAALAWLQSRPGMDTPRHRWLIRLL
                     MARVAEQYGRNDMALHLLGELITRAPQLTLEDWEPALLFEVRARRLKLLRMKAGRSES
                     DKARLMPEMDTLLAGLIAIDPARAMVLCG"
     gene            2931..>4541
                     /gene="tssF"
                     /locus_tag="ACHY09_004724"
     CDS             2931..>4541
                     /gene="tssF"
                     /locus_tag="ACHY09_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS3887575.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     GVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYMRTIPSLAIVEFSPDWRSL
                     RQAEILAEGFSVLSRPVGPHKTACQYRTTRDVLLQPLHLADARLHTESDGRSAIRLRF
                     ECPEKVDWSKAGIDKVAIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGW
                     CRPMGFDDNDCLWKKADTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAASTWFEI
                     DIVLSEAWSSCLPFETENFRLHCAPVINLFTLEADPLLLNPLDNEYLLRPLRLQDGHT
                     EIYSVDNIHGSVKNGRHPYVPFTSFRHRGGMMRHDAPERYYHTRVKRGPSGLYDTWLV
                     LGGRSFELEQLSDKPESLSMRITATNGQLPRRALESTLLDRVVKTGKVPVRVLNLTAP
                     TMPLYPPANDRFHWRVMSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRRRLEAIVA
                     VKHTLIRRFEKGFMLRGVDIEVTLNMDNF"
BASE COUNT          931 a         1243 c         1353 g         1014 t
ORIGIN      
        1 acggtaatct gagcttccgg tcctggctcg cccgtgtaac ccagatgcgg ctgaaacttc
       61 agcaggttac cagcgccccg gatccgcagg cgatgtcgca gatgctggcc cgggccgtgt
      121 ttcagggcaa agccatcgat ctgacggaca cctgcgatta cggcagcctg gttgcagcaa
      181 gtctggggca ggaatggagt ggatttggtc agtcgttgtt tgtgcaaccg ctggatctgg
      241 cctggcggca ggtgcttgtc ccggctgcgg gcagcctcaa tgcccgctgg cagacaacca
      301 tagtcgatca gtggaataag gcttttgcgg ggcggtaccc gtttaaagcc accggcagtg
      361 atgcctccct gccgttactg gcacagttcc tgcgtagcga ctcgggtcgg atcacgacgt
      421 tcctgaaaac tcatcttggc gggctcgtcc accaggaggg gaatcactgg gtcgctgagc
      481 cttccgcaag ccaggggatg gttgtcagtc cggatttcct ccgggcgatt aatcagctgg
      541 cagagctgtc agatatcgtg ttctctcagg gcgatgcggg ggttcacttc gaactgatgg
      601 cgcgtccttc acgggatgtt gccagagtgc agcttaccct tgatgaacag aatctggatt
      661 acttcaatca gatggaaagc tggcagagct tcacctggcc ggggaatact tactatccag
      721 gtgtgtcgct cagctggcgg agtgtaagca gtggtatgca actctacgcc agtcaccagg
      781 ggaactgggg gtttattcgc ctgctggacg aagccctgat cacgccactt gatgccagcc
      841 gcacacaact ggtgtggatc actgccgatg gtaatcctct gaaattcgtc atgcgcagcg
      901 agctgggcga cggtccgctt gccttactga agctacaggg gtttacgctg cccaccgcca
      961 tttttaacgt tgccgccggc acggaaggtg ttgagtaaaa atgaacaggg gcatttatcc
     1021 gacgccggcc tttactgaag atctgggggc tgatgggcac atttttcacc ctttcagaga
     1081 acggctcctt catgcatgcc cgtcggcgtt ttcgcccaca tgtcagaaaa aacaaccgat
     1141 aagctgacgg aagggcaaac ccactcagca ggagaacgta tgctgctgaa atggaggcca
     1201 ccacaaatct tcaacagtaa ggacgacgct tctccggaag cggtcgtgag gtttatgaaa
     1261 gagactgata aacaggaaaa agaatgagtt caccggaagc cctgttaacg gcctgcgccg
     1321 ccagccagga agaaaggctg agcctcattt cccgcgcaaa tgaggccctg tcgctctggg
     1381 ataactggct gcatccattt acctccggat ctgaaaccgg ggacgatcct gcatacgatg
     1441 ataattttca gctgatgcgt gaagagatca ataagatttc aggtactgat tcagcgctgt
     1501 tgtgcgaact ggctcagaag tgcctttgcg aatgcgccag agatattcgt gtcgtgacct
     1561 ggtacgtgct ggcccgtctc acccgggatg gtgaaagagg gttgtccgaa ggcttattac
     1621 ttcttgttgc gatgctgacc cgctacggac aggcatgtca tccccggcgt cctgccgccc
     1681 ggaaagctgc gctggagtgg ctgaacagtg caaaagtgac tgatgcactg ctgttatggc
     1741 ccggggtgga cagcaatgag gccggtctga cagcgggtgc cataagcctg ctgggatccg
     1801 ccgtggcaga ctggccggaa gcggagaagc cgtcttttgc ggggctttgt tccgcgcttg
     1861 agaaccgcct ggcacgttca gggggcatgg aggcactggt cccccagaac agcagtcccc
     1921 gggagaccgg gcgtgaatca gctcccccgg actcaccaca actggcagcg gtgacctcgg
     1981 ggcgcgactt actcgaccag gccaggttac tttcccgctg gctgagcggg cagccgcagg
     2041 gctggctggc ttctcaccgg ctgatgaaga cggtgcggtg ggatacggtc gatcagctcc
     2101 cttcgctgga cagtagcgga cgcacccgcc tggtcccccc gaaaaccggg tatcgcgctc
     2161 agcttaagcg actctggctt cagaaaaact gggttgagct gattgaactg gccagtcaga
     2221 tgtattgcga aggtgtgaat catttctggc tcgatcttca gtggtatctc tggcagggac
     2281 tcagtcatgc tggtcagccc tgggattcct ggtctgacgc tgtcctgtca gatctgcggc
     2341 tgttgcttaa acgtctcccg ggccttgaag gactggcatg gaatgacggt actccctttg
     2401 ctgacgaggt caccctcgcc tggatcgctg aaaaagtgaa tgaagaagat gtgatgtacg
     2461 gcgatgaagc tgccattcct gccgttgtta atggccagac tgacgatgtg ctgatccttg
     2521 agtctgaagc catggaaaag ggagacacgg aagggcctga agcggcgctt gcctggctcc
     2581 agtcccggcc ggggatggat acaccacgtc atcgctggct gatacgcctg ctcatggccc
     2641 gggtggcaga gcagtatggc cggaacgata tggcgcttca tctgcttggt gaacttatta
     2701 cccgtgcgcc acagctgacg ctggaagact gggagcccgc tttgctgttt gaagtccggg
     2761 cccgacgcct gaagctgcta cggatgaaag ccggtcgcag cgaatctgat aaagcccgtc
     2821 tgatgcctga gatggatacg ctgctggcgg ggctgatcgc cattgacccg gcccgtgcca
     2881 tggtgctctg tggctgatgt tctctttaat cattttcagg aatgccgtgc atggatgatt
     2941 taaccctgcg ctattacgaa gcagagatgc gctatctgcg ggaagccggt aaagagtttg
     3001 cccgtgctca tccggatcgg gcagcaatgc tgaatctgga taagcctggc gcctgcgatc
     3061 ccggtgtgga acgtctgttt gaaggctttg ccttcctgat gggacgtttg cgtgaaaagc
     3121 tggatgatga cctgccggag ctcaccgaag ggctggtgag tctgctgtgg ccgcactata
     3181 tgcggactat cccgtcgctg gccatcgttg agttctcgcc tgactggcgt agtctgcgtc
     3241 aggccgaaat actggctgaa ggcttctccg ttttgtcccg tcctgtcggg ccgcataaaa
     3301 ccgcctgtca gtaccggacg acccgcgatg tgttgctaca accgctgcac cttgcagatg
     3361 cccgtctcca tactgaatcc gatggccgct ctgccatccg cctgcgcttc gaatgccctg
     3421 aaaaagtgga ctggagtaaa gccgggatcg acaaagtcgc catttttctt aatgcagagg
     3481 cgccggtaag cgctgctctg catctggcaa tgacccggcg tgtacatgcc atgtatgcgc
     3541 gtcatgccgg gacatatacc ggacgtcatc agtttgacgg ctggtgcaga cccatgggct
     3601 ttgacgacaa tgactgcctg tggaagaaag ccgatacagc cttcagtggt taccagttgc
     3661 tgctggagta tttcagcttc cgtccgaagt ttatgtttgt tgaactgcgc ggcctcgaca
     3721 ccatcgggct cactgcggcc agtacctggt ttgaaatcga cattgtgctc agtgaagcct
     3781 ggtcatcctg tttgcctttt gagacagaaa acttccgcct gcattgtgcg ccggttatca
     3841 atctctttac cctggaagcc gacccgctgc tccttaaccc gctggataac gagtacctgc
     3901 tcaggccgct gcgtcttcag gatggtcaca ccgaaattta cagcgtcgat aacattcacg
     3961 gctcggtgaa gaatggcaga cacccttatg tcccgttcac cagcttccgg caccggggcg
     4021 gtatgatgcg ccacgatgct cctgaacgtt attaccacac ccgcgtgaaa cggggaccct
     4081 ccggcctgta tgacacatgg ttagtcctcg ggggacgttc gttcgaactg gagcagttgt
     4141 ctgataaacc ggagtccctc tcgatgcgaa tcaccgcgac caacgggcag cttccgcgca
     4201 gggcgctgga aagtacgctc ctggacaggg tggtcaaaac cggcaaagtc cccgtcagag
     4261 tcctgaacct gaccgcaccg acgatgccac tgtatccccc ggcaaatgac cggtttcact
     4321 ggcgggtgat gagtcacctc ggttcaaact ttctcagcat gatggacaat ccggaggtgc
     4381 tgcgggggac gctggcgctc tatgactgga ccgacgatga aatgaaccgc cgccggcttg
     4441 aagccatcgt tgccgtgaag cacaccctga tccgccgttt tgaaaagggc tttatgctcc
     4501 gtggcgtgga cattgaggtc acgctgaata tggataattt t
//
LOCUS       DAZUCC010000294         4526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZUCC010000294 DAZUCC010000000
VERSION     DAZUCC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.296"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..293)
                     /gene="chbB"
                     /locus_tag="ACHY09_004725"
     CDS             complement(<1..293)
                     /gene="chbB"
                     /locus_tag="ACHY09_004725"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFS3887576.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVA"
     gene            complement(592..930)
                     /gene="osmE"
                     /locus_tag="ACHY09_004726"
     CDS             complement(592..930)
                     /gene="osmE"
                     /locus_tag="ACHY09_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFS3887577.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1132..1959
                     /gene="nadE"
                     /locus_tag="ACHY09_004727"
     CDS             1132..1959
                     /gene="nadE"
                     /locus_tag="ACHY09_004727"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS3887578.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2189..3076
                     /gene="cho"
                     /locus_tag="ACHY09_004728"
     CDS             2189..3076
                     /gene="cho"
                     /locus_tag="ACHY09_004728"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFS3887579.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3036..3611)
                     /gene="ves"
                     /locus_tag="ACHY09_004729"
     CDS             complement(3036..3611)
                     /gene="ves"
                     /locus_tag="ACHY09_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFS3887580.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3814..4299)
                     /gene="spy"
                     /locus_tag="ACHY09_004730"
     CDS             complement(3814..4299)
                     /gene="spy"
                     /locus_tag="ACHY09_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFS3887581.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTSAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1137 a         1097 c         1080 g         1212 t
ORIGIN      
        1 gcaaccgcag ccttaagcac gcctaaacca tcgactttgc cataaagcag cgagtcaatt
       61 acttcgaccg gtttgttggg taacaaacgc tggatttcgg gcaacatata agcaatctgc
      121 ggccctaata acacgacatc ggcattctga cctttttcac cagccagtgt ttccggaaat
      181 gcttcaataa tgaccggaac ttcatatttt tctgcctgtg cgcgcatttt tgataccagt
      241 aaagaggtag acatgcccgc agaacaaaac agataaatgt gtttcttttc cataaaactg
      301 ccctcatcga cgattatctg tcagccagac actccgcaag ccttaacctg cttccatgct
      361 ctgggtaact tgcgaaacca acatgacttt ttttgtggct gaaaggagta tacggtatag
      421 acctgagcaa tagtatttct ttgacctgat aaattgtctc tccttgacct gcggttatga
      481 ccctcttcac atttcgggca aataattcgc gaagataatt aagaactcag gccaaaaaaa
      541 accggcgcaa tggccggttt ccgttgtaac tcaagctttc aggcgaagtg attacttcgc
      601 agcctgtgga tcagtgtcgt attcagcaca ggtctgataa ccggagttga tgacatgtcc
      661 ggtatcatct aacgcgacaa agtaggtttc tgctttacca tcacgttgac ccaggatgta
      721 ggtctggcaa gtaccacgag catggatcat gctcacttca gacgaaggtt tacccgcaat
      781 ttgtgcaacc tgcgcccggc tcatgccttt tttgacgtct ttcaccacag gctgtacaaa
      841 ctggtctttg gtacgatcat aagccgtaca acccgccagc atggttaata ccgccgctgc
      901 actcagaatt cctgccatat tcttgttcat attccgtcct cttgtttatc agcgtgttag
      961 ataagcctgg aatacattgg gcgctttttc aagcccgtga acgaaacggc tccgctttca
     1021 gaggattcct gtatgacgtt ttaaccacca ttcagcccgc tgtcgcttgt cgtttcagta
     1081 gcaacgggtt agctttaagg aagttttgtc ttttctgtct ggaggggttc aatgacattg
     1141 caacaacaaa taataaaggc gctgggcgca aaaccgcaga ttaatgctga agaggaaatt
     1201 cgtcgtagtg tcgattttct gaaaagctac ctgcaaactt atccgttcat taaatcactg
     1261 gtgctcggga tcagcggcgg tcaggactcc acgcttgccg gaaagctgtg ccagatggcg
     1321 attaatgagc tgcgccagga aactggcaac gaatcactgc aatttattgc cgtacgcctg
     1381 ccctatggtg ttcaggctga cgaacaagat tgccaggatg ccattgcctt tattcaaccg
     1441 gatcgcgtat taaccgttaa tatcaagggc gcggtattgg ccagcgaaca ggcgctgcgc
     1501 gaagcgggta ttgaattaag tgattttgtc cgtggcaatg aaaaagcgcg tgagcggatg
     1561 aaagcacaat atagcattgc gggtatgacc agcggtgtcg tggtgggcac cgatcatgca
     1621 gcagaagcca ttaccggatt cttcactaaa tatggtgacg gcggtacgga cattaatcca
     1681 ctgtatcgtc ttaacaaacg tcagggtaaa cagttactgg cggcattagg ttgcccggaa
     1741 cacctttata agaaagcgcc aacggccgat ctggaagatg atcgcccttc tctaccagat
     1801 gaagtggcac tcggcgtgac ctatgacaat atcgacgact atctggaagg gaaaaacgta
     1861 cctgaacagg tcgccagaac aatagagaac tggtatctga aaactgaaca taaacgccgt
     1921 ccgccaatta ccgttttcga tgatttctgg aagaagtaat aatttgcaca tattggtttg
     1981 tgcgaaaaag agtactttgt tcacgccgga tgcggcgtga acgccttatt cgccccacaa
     2041 aactatgcaa attcaatata ttgcatgagt cgaataggcc tgacaggcgt agcacgtctg
     2101 acggtgtaac ctttgtcatc aacccgcctc ttttttaatc gctccccgcc tgttacactg
     2161 gatagataac cagcattcgg agtcaactgt ggtacggcgt ttaacttctc cgcggctcga
     2221 atttgaagct gcggcaattt atgaatatcc cgaacattta cgttcattcc ttaatgactt
     2281 acccacccga ccaggggtgt atctgtttca tggcgaaagt gacaccatgc cgctctatat
     2341 cggcaaaagc attaacattc gcagccgcgt cctttctcat ttacgtaccc cggatgaagc
     2401 cgccatgcta cggcaatccc gacgcatcag ctggatatgt accgccggtg aaatcggcgc
     2461 tctactcctt gaagcgcgat taatcaaaga gcagcagccg ctgtttaata aacgcttgcg
     2521 ccgcaatcgc cagctctgtg ccctgcaatt aaatgaaaag cgcgtcgatg tggtgtatgc
     2581 caaagaggtg gatttttcac gagcccccaa cctgtttggc ctgtttgcca ataggcgcgc
     2641 agctttgcaa gcattgcaga ccatcgctga tgaacaaaaa ctttgttatg gtctgttggg
     2701 actggagccg ttaagtcgcg gtcgtgcatg ttttcgttca gcgctaaaac gttgcgccgg
     2761 agcatgctgc ggtaaagaga gccatgacga tcatgcgcta cgtttgcgcc agtctctgga
     2821 acgtttacga gtagtttgtt ggccgtggaa aggtgcggtg gcgctgaaag aacagcaccc
     2881 ggaaatgact caatatcata ttattcaaaa ctggctgtgg ctgggggcgg ttaattcgct
     2941 ggaagaagcg acaatgttaa ttcggacacc cgccgggttt gatcacgacg gttataaaat
     3001 tctttgtaag ccgctgcttt ccggtaacta tgaaattact gaacttgatc cggcgaatga
     3061 ccagcgagcc agttgatttc actgaacaac agtttacctt gcggttgtaa aagccgcagg
     3121 gtgtgcctgc catcgaacca gcacgcgccc tggtcggtag tgagcagttt atcgcccaat
     3181 tgccacgccc cgttaatgac aaacactacc ccgccacgtg agccaaaggt agtaaacgta
     3241 cgttcggcaa ttctgacttt cgccttacag acatccaggc gtgtcatgat gttgaaatcc
     3301 atcgacatct gccctgccgt cagtttcgct tttaccacct ggtccgccgc aaaggcaaaa
     3361 ggctgtaacg gttttaaggt atggttaaag cggtctgtgc tttcaaggaa catctcgccg
     3421 ccttccagca acgtcactat cctttccata cctggaaaca aagaaaactc cccattcgcc
     3481 gcgatggatg caatgctggc acgccagtaa aaatcacgtt ttgccggtgg gaaagtgcaa
     3541 atttcgcgcg tttcaccggc agcatttcgc cacagattca ccgacatttt acgcatatca
     3601 aagtattcca tactcgctcc cggcccgttc ttttcattgt tatcgttcag gtaaccgtgg
     3661 atggttatcg gcaactcggg tgatttacgt tagtggcgat caggcttttt ttgcatctgt
     3721 ttgagaacaa aaaagaaaac cgccgatcct gtccaccgca ttactgcaag gtagtggaca
     3781 agaccggcgg tcttacgttt tttggctgaa agattattca gcagttgcag gcattttacc
     3841 ttttgccgct ggacgttctg tcagacgctt ctcaaaatta gcattaaatt gctttttctg
     3901 ttccggcgtc aagatgttgt aaatcttgtt ctgggtttcc atgtgcgcca gcatgttagc
     3961 tttgcgctgt tcttccattt ttgcgatctg cgcttcagct tttgctttat cgaaggtatc
     4021 gctggcaatg atgtcatgca ttgcgcggcg ttcttccagc ggcggacgtt tcatctggtc
     4081 acgctggcct ttcatgattt cgcggatctg ctgtttctgc gcgtcggtca ggttcaggtc
     4141 tttgaacatc atgtcctgat gcggaccgaa cttgcctttg tggtgcatca tcggcttcgc
     4201 gtcagccggt gctgaggtag tggtgtctgc ggcatgggcc aggttagccg cgccaagagc
     4261 cagggtagag gcaacaaaca gtgcagttaa tttacgcata ttctatatcc ttcctttcag
     4321 ttatttatta cggctttctt aagtagcgtg ccgtgttgac gagattaact ttactgactt
     4381 tagcgtcaat taatcagagc aacggtaaaa caatgaaagt gtaaaaaaca cttttgcgcc
     4441 aattatggag aaaaaaagaa aatttgatgg agagtaatga gagaatatta caacacgatg
     4501 attttgcaga gattatgaag aactat
//
LOCUS       DAZUCC010000295         4512 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZUCC010000295 DAZUCC010000000
VERSION     DAZUCC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.297"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..900
                     /locus_tag="ACHY09_004731"
     CDS             <1..900
                     /locus_tag="ACHY09_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887582.1"
                     /translation="LLEAEMKLMRCMATVRAWTPEMRAGSGVVVSWRYGNVCVQRGVQ
                     LRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPDKSLALLA
                     WTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKDGAAFMAMMAEKARLAALLPEG
                     WARDMTTFLSLSQDVLLSLLSFCTACSIHGVQTREYGHTSRSPLDSLESAIGFHMRDW
                     WQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEFHMKDNRWVPGW
                     MCTPRPQAETETTEYRDDQAEAA"
     gene            969..1403
                     /gene="psiB"
                     /locus_tag="ACHY09_004732"
     CDS             969..1403
                     /gene="psiB"
                     /locus_tag="ACHY09_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFS3887583.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDEQGYSVSDIIHILMAEGGQA"
     gene            1400..2119
                     /locus_tag="ACHY09_004733"
     CDS             1400..2119
                     /locus_tag="ACHY09_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFS3887584.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSVLVMQKYSRQQAREAEQKARAYQTLVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            2116..2430
                     /locus_tag="ACHY09_004734"
     CDS             2116..2430
                     /locus_tag="ACHY09_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFS3887585.1"
                     /translation="MKWRYSLRWKLPSPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            2580..2732
                     /locus_tag="ACHY09_004735"
                     /pseudo
     CDS             2580..2732
                     /locus_tag="ACHY09_004735"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            2677..2799
                     /locus_tag="ACHY09_004736"
     CDS             2677..2799
                     /locus_tag="ACHY09_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3887586.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
     gene            complement(3253..3426)
                     /locus_tag="ACHY09_004737"
                     /pseudo
     CDS             complement(3253..3426)
                     /locus_tag="ACHY09_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3726..4031
                     /locus_tag="ACHY09_004738"
     CDS             3726..4031
                     /locus_tag="ACHY09_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887587.1"
                     /translation="MSAFSFNTAYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLLKQLWQINDELGELLDSVVENHTPVIRKKASDLSALMMLFSQQAGQPMTMLLIC"
     gene            4187..4474
                     /locus_tag="ACHY09_004739"
     CDS             4187..4474
                     /locus_tag="ACHY09_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887588.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRTGLDIRAIC"
BASE COUNT         1006 a         1209 c         1371 g          926 t
ORIGIN      
        1 ctgctggaag cagaaatgaa gctgatgcgc tgcatggcga cggtcagggc gtggacgccg
       61 gagatgcgtg ccggaagcgg tgtggtggtg tcctggcgtt atggcaacgt gtgtgtccag
      121 cgtggtgtgc agttgcgcag cgaggatgac gcggctgccg acgctgaccg cacggaacag
      181 gtgcaggaga aagcgtcagt ggaggaaatc agtctgccac tgctgacgaa aatgtcttcc
      241 gaacgcacgc tggcagtcca ggcggcactc atgcagcaac cggacaaatc tctggcactg
      301 ctggcatgga cgctctgcct gaatgtgttt ggcagcggag cgtacagtaa accagcacaa
      361 atcagcctgg aatgtaaaca ttattcgctg accagcgatg cgccatcggg gaaggacggt
      421 gccgcattca tggcgatgat ggcagaaaaa gcccgtcttg ctgccctgtt accggaggga
      481 tgggcacggg acatgacgac gttcctgtca ctcagccagg acgtgctgtt atcactgctc
      541 agtttctgca ccgcgtgcag catccacggc gttcagaccc gcgagtatgg tcacacgtca
      601 cgcagtccgc tggactcgct ggagagcgcc atcggctttc acatgcgtga ctggtggcag
      661 ccgacaaaag caaacttctt cggtcacctg aaaaagccgc agattatcga tgccctgaat
      721 gatgccggac tgtccggtgc cgcacgggac gcggagaaga tgaagaaagg cgatgccgct
      781 gaacatgcag agttccacat gaaagacaac cgctgggtgc ctggctggat gtgtacacca
      841 cgtccacagg cagagacgga aaccacggag taccgtgacg accaggcaga agccgcctga
      901 ccgccagtca taaaccattt atcacgccgc cccgccggag acggggcggc agcaagggag
      961 ataccgtgat gaaaactgaa ctgaccctga atgtattaca gaccatgagc gcacaggaat
     1021 atgaagatat ccgggctgcc ggaagtgatg aacgccgtga gctgacacac gccgtgatgc
     1081 gggaactgga tgcgccggac aactggacga tgaatggcga gtacggcagc gagttcggcg
     1141 gctttttccc cgtccaggtc cgtttcacgc ctgcccacga acgttttcac ctggcgttat
     1201 gttcaccggg cgatgtctct caggtctggg tgctggttct ggtgaatgcc ggtggtgagc
     1261 cgtttgcggt ggttcaggtg cagcggcggt ttgcaccgga agctgtcagc cattcactgg
     1321 cactggcggc atcactggat gaacagggat acagcgtcag tgacatcatc catatcctga
     1381 tggcggaagg aggtcaggca tgagcgcacg ttcacaggca ctggttcctc tcagcacaga
     1441 gcaacaggcc gcatggcggg cggtggcgga gacagaaaaa cgccgccacc agggtaacac
     1501 gctggcggaa tatccgtatg cgggggcgtt tttccgctgt ctgaacggaa gccgcaggat
     1561 ttcgctgtct gacctgcgat ttttcatgcc gtccctgacc gcagaagaac tgcgcggcaa
     1621 ccgttctcag tggctgtatg ccgtcgatgt gctgattgaa acacagggcg aggtctgcct
     1681 ccttcccctt cccggtgatg cagcagagcg gttgttcccg tcggtgcggt tccgtgtcag
     1741 ggaacgcagt cgccataaat ccgtgctggt catgcagaag tacagccgcc agcaggcacg
     1801 ggaggcagag cagaaagccc gtgcgtacca gacgctggtc gctcaggctg aaatcgaact
     1861 ggcgttccat tcaccggaaa cagtcggaag ctggtatgca cgctggagtg accgggtggc
     1921 agagcatgac ctggaaaccc tgttctggca gtggggtgag cgttttccgt cactggccgg
     1981 gatggaacgc tggcagtggc aggatatgcc attctggcag gtcatcgcag aggcaggtat
     2041 ggcagcaagg gaggcgggtc acgctgtccg tgagatggag cggtggatgg tgccgaacaa
     2101 actccgggag gcggcgtgaa gtggcgttac tcactgcgct ggaaactgcc ttctccctgt
     2161 cctggcgagc atgaactggt ttcagaagtg gtggaggcgg ggcagcctgc accggcatcc
     2221 gttatgtcac gctgggttgc cggagcaggg tatgcggtct gtctggattt tatttctgac
     2281 cgcccggtgc gccgctggag cgaagaacgt aaggctgcgg tacggcggcg gaatctggag
     2341 aaacgtatca acaggcatgc accactgttt gcggatgaac tgattgcccg tgaactggct
     2401 gaacgaccgg attattttca gggaaagtga gtgtggtcag ttcgcaggta tatgggctat
     2461 gatgtgcccg gcgcttgagg ctttctgcct catgacgtga aggtggtttg ttatcctgtc
     2521 gtgtggcaga aggacaaaag ccccgtagtt aatttttcat taacccacga ggcctctgca
     2581 tgtctagtcc acgtcagtat ggcctcttac cgtgcaaatt gcaaggagga gtaaaccatg
     2641 aaactaccgg gaaacgccct tatctggtgc gtattaatcg tgtgctgcac gttgttgata
     2701 ttcacactcc tgacccggaa tcgcctgtgc gaagtccggc tgaaggacgg atacagggaa
     2761 gttacggcaa caatggctta cgaatccggc ggtaagtagc aacctggagg cgggcgcagg
     2821 cccgcctttt cagggctgat gctggtctga ctgcactgaa gcgcctttat aaaggggctg
     2881 ctggttcgcc ggtggcccct ttctccttgc tggcattgta gcagcatgaa caaccagact
     2941 gtcaggaagg ccacttccct ccgggaacgg gaatgaacgt gcgcctgcgg cgctcgttct
     3001 tttcccccgc cttctctgtt tatggccagt tgtcagaaat gacattacct ttcccgtgat
     3061 tattctcttt ttcttcctgc tctgattctg actactgcag tgttgtcctg tctgccgggc
     3121 gcaccttcgg ctcccggctg cctggcggca tccggtcgtc aggcccgact ccgacaggca
     3181 gctgctggcg cacctgccag tctccgccga acccgctggc gcggtttcgg gctgccgcta
     3241 cggtccgatg cgtgaaaacc ccgggcttgc agcgaccgtt ccggtcgcgc cgcctctgtc
     3301 gcgccgtggc ctgcgctccc ggcatcccgg caaaaagacg ccgggatgta aggcgcaacg
     3361 ccgtttcgca aaccgcgctt cacgtcgttg cggcgggtcc ctgcccgttg ttctgtccgg
     3421 tgccattcac ctgtttaagc ccctgtcgcc gggggctgat acagccacaa cctgccgcac
     3481 tgactgtcca gggtaaatga acagcccaca aaatttttta cccaaaaccc ggtaaaaaat
     3541 tttgcgttct gcccaggaca ggtgcgtcag gccgtggcag tgatgcccca tgcggcgaag
     3601 gttctcaaac aggtgatggt gaataccgga gggaacaaaa ggcaggtccc cgcaaaacgt
     3661 caaaaccgcg gctcccgaaa ctcaacagat gatgggggct gaaaacatca gatacaggag
     3721 gaaacatgag cgcattttct tttaacaccg cttatcagcc aacaggcgat cagcagaaag
     3781 acgcaatcgc acaaatcgac atcatgcaga atcgtgctgt tcaggcaaat cttgcatacc
     3841 agagcagcct ggatgcggaa acgctattga aacagttatg gcaaatcaat gatgaactgg
     3901 gggaactgtt ggatagcgtg gtggaaaatc acacgccagt tatcagaaaa aaagccagtg
     3961 atttgagcgc actgatgatg cttttcagcc aacaggccgg tcagcctatg actatgttgt
     4021 tgatttgtta atgataatca ggggcaggaa attaagcgaa agatgctctg ccccgggttt
     4081 gcagcgaccg gaacggtcac gccacccttg tcgcgctgtg gcctgcggcg gctcccgaaa
     4141 ctcaaccgat gatggggtct gaaaaccaga gagaggagaa agcaggatga gtacccgaaa
     4201 tatccacgtt aacaccgcgt cgtacacgct tcttgttgcc gggaagaaaa aaaacaccgg
     4261 ggaagagtgg gacgtcctgg aattcagcag cctgactgag ctgaaaaaat atcgcaaaag
     4321 ccacccggaa aagatggcct tcagctatag ctacgcgctc agccggggtg tggatacgca
     4381 gttccgccat atcaacattg cggaagccga tcacttcaaa cagttcctgc gtcagattaa
     4441 gcgtaccggc ctcgatatcc gggcgatctg ctgaccggat gtgatgaata tccctcctga
     4501 ttgttgcaat ct
//
LOCUS       DAZUCC010000296         4459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZUCC010000296 DAZUCC010000000
VERSION     DAZUCC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.298"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..75
                     /locus_tag="ACHY09_004740"
     CDS             <1..75
                     /locus_tag="ACHY09_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887589.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
     gene            336..677
                     /gene="raiA"
                     /locus_tag="ACHY09_004741"
     CDS             336..677
                     /gene="raiA"
                     /locus_tag="ACHY09_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS3887590.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    780..905
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            781..828
                     /gene="pheL"
                     /locus_tag="ACHY09_004742"
     CDS             781..828
                     /gene="pheL"
                     /locus_tag="ACHY09_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFS3887591.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            927..2087
                     /gene="pheA"
                     /locus_tag="ACHY09_004743"
     CDS             927..2087
                     /gene="pheA"
                     /locus_tag="ACHY09_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFS3887592.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPQWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2130..3251)
                     /gene="tyrA"
                     /locus_tag="ACHY09_004744"
     CDS             complement(2130..3251)
                     /gene="tyrA"
                     /locus_tag="ACHY09_004744"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFS3887593.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAAEIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(3262..4332)
                     /gene="aroF"
                     /locus_tag="ACHY09_004745"
     CDS             complement(3262..4332)
                     /gene="aroF"
                     /locus_tag="ACHY09_004745"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFS3887594.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         1197 a         1166 c         1021 g         1075 t
ORIGIN      
        1 gccttcccga gagtggtaaa ggagaggccc tggaaatacc ccacagcccc gaaaaagagc
       61 cagtcagttg cttaactgat cggcattaca gcccttgagc tgccgttttt ttattctgtt
      121 agttgtgaaa ctgaagcgat ttagtcacta tcgatctcat caaatatggc tcgctttgag
      181 atattcctca agtaaaaaaa cacctcttcc tgcgatttct cacaaaaaag attcgttgac
      241 aaaaagtgac aaaattatga gatttccatc acacattttg acatcaggaa cggtatgctg
      301 aattcaccaa gacgggaaga caagaggtaa aatttatgac aatgaacatt accagcaaac
      361 aaatggaaat tactccggcc atccgccaac atgtcgcaga ccgtctcgcc aaactggaaa
      421 aatggcaaac acatctgatt aatccacata tcattctgtc caaagagcca caagggtttg
      481 ttgctgacgc cacaatcaat acacctaacg gcgttctggt tgccagtggt aaacatgaag
      541 atatgtacac cgcaattaac gaattgatca acaagctgga acggcagctc aataaactgc
      601 agcacaaagg cgaagcacgt cgtgccgcaa catcggtgaa agacgccaac ttcgtcgaag
      661 aagttgaaga agagtagtcc tttatattga gtgtatcgcc aacgcgcctt cgggcgcgtt
      721 ttttgttgac agcgtgaaaa cagtacgggt actgtactaa agtcacttaa ggaaacaaac
      781 atgaaacaca caccgttttt cttcgcattc ttttttacct tcccctgaat gggaggcgtt
      841 tcgtcgtgtg aaacagaatg cgaagacgaa caataaggcc tcccaaatcg gggggccttt
      901 tttattgata acaaaaaggc aacactatga catcggaaaa cccgttactg gcgctgcgag
      961 agaaaatcag cgcgctggat gaaaaattat tagcattact ggcagagcga cgcgaactgg
     1021 ccgtcgaggt gggaaaagcc aaactgctct cgcatcgccc ggtacgtgat attgatcgtg
     1081 aacgcgattt gctggaaaga ttaattacgc tcggtaaagc gcaccatctg gacgcccatt
     1141 acattactcg cctgttccag ctcatcattg aagattccgt attaactcag caggctttgc
     1201 tccagcaaca tctcaataaa attaatccgc actcagcacg catcgctttt ctcggcccca
     1261 aaggttctta ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt gagcaattca
     1321 ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca ggtggaaacc ggccaggccg
     1381 actatgccgt cgtaccgatt gaaaatacca gctccggtgc cataaatgac gtttacgatc
     1441 tgctgcaaca taccagtttg tcgattgttg gcgagatgac gttaactatc gaccattgtt
     1501 tgttggtctc cggcactact gatttatcca ccatcaatac ggtctacagc catccgcagc
     1561 cattccagca atgcagcaaa ttccttaatc gttatccgca atggaagatt gaatataccg
     1621 aaagtacgtc tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt
     1681 tgggaagcga agctggcggc actttgtacg gtttgcaggt actggagcgt attgaagcga
     1741 atcagcgaca aaacttcacc cgatttgtgg tgttggcacg taaagccatt aacgtgtctg
     1801 accaggttcc agcgaaaacg acgttgttaa tggcgaccgg gcaacaagct ggtgcgctgg
     1861 ttgaagcgtt gctggtactg cgcaaccaca atctgattat gacccgtctg gagtcacgcc
     1921 cgattcacgg taatccatgg gaagagatgt tttatctgga tattcaggcc aatcttgaat
     1981 cagcggaaat gcaaaaagca ttgaaagagt taggggacat cacccgttcg atgaaggtat
     2041 tgggctgtta cccaagtgag aacgtagtgc ctgttgatcc aacctgatga aaaggtgccg
     2101 gatgatgtga atcacccggc actggattat tagtgacggt tgtcattcgc ctgacgcaat
     2161 aacacgcggc tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg
     2221 cggaaactgt caataaacgc ctgcttatcg ccctgctcca gcaactcaat cgcctcgccg
     2281 aaacgcttat agtaacgttt gattaacgcc agattacgct ctgacgacat aataatgtcg
     2341 gcatacagtt gcggatcctg ggcaaacagt cgcccaacca tcgccagctc aaggcggtaa
     2401 atcggcgaag aaagcgccag aagttgctca agctgaacat tttcttctgc caggtgcagc
     2461 ccataagcaa aagtagcaaa gtggcgcagt gcctgaataa acgccatatt ctgatcgtgc
     2521 tcgacagcgc taatacgatg cagccgagcg ccccagacct gaatttgctc cagaaaccat
     2581 tggtatgctt ccggcttacg tccatcacac cagaccacaa cttgttttgc caggctaccg
     2641 ctgtccgggc cgaacatcgg gtgtaacccc agcaccggac catcatgcgc cgccagcatg
     2701 gcctgtaatg gtccattttt caccgatgcc agatcgacca gaatacaatc tttcggtaaa
     2761 ggcggtaatt tgccaataac ttgttcagta acgtggatgg gcacactaac aatcaccatt
     2821 ccggcatcgg caactatctc agccgctcga tcccagtcat gttgctccag aatccgcacc
     2881 tgataacccg atagtgtcag catcttctcg aacaggcgtc ccatctgacc gccaccgccg
     2941 acgataacca ccgggcgcag tgacggacaa agcgttttaa atcctttgtc gttttcactg
     3001 gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg cggtacaccc
     3061 agagcttccg cctctgcacg acgcgaggcc aacatagatg cctcgcgctc cggaacataa
     3121 ataggcagtc caaagcggct tttcacctcg cccacttcag caaccagttc cagacgcttc
     3181 gctaataaat tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa tgcggtcaat
     3241 tcagcaacca taataaacct cttaagccac gcgagccgtc agctgcccgt tcagatcctg
     3301 atgaatttca cgcagcaagg catcggtcat ttcccagcta atgcaggcat cggttacgga
     3361 tacaccgtat ttcatttcac tgcgcggttg ctcggaagac tgattgccct cgtggatatt
     3421 actttcgatc atcagaccaa taattgagcg attgccatct ttgatttgag caaccacgga
     3481 ttctgccacc gcaggctgac ggcgataatc tttattggaa ttaccgtggc tgcaatctac
     3541 catcagagac gggcgcagtc ccgcctgttc catctctttt tcacattgcg caacatccgc
     3601 agggctatag ttcggcgctt taccaccgcg caggatcaca tgcccgtccg gattcccctg
     3661 agtttgtagc aacgccacct gccctgcctg gttaatgcca acaaaacggt gcggctgggc
     3721 ggcggcacgc atagcgttaa tagccgttgc aaggctgccg tcggtgccgt ttttaaaacc
     3781 aaccggcatg gaaagcccgg aggccatttc acggtgtgtt tgcgattccg ttgtacgagc
     3841 accaattgct gaccagctaa acagatcgcc caggtattgc gggctattcg gatctaacgc
     3901 ttccgtcgcc agtggcagtc ccatattcac cagctcaagc agcaatttac gcgcgatctg
     3961 cagcccggct tctacatcaa aagagccatc catatgggga tcgttaatca accctttcca
     4021 accgacagtg gtacggggtt tttcaaaata gacgcgcatt accagataga ggctatcgct
     4081 gacctctgcg gcaagggctt taaatcgacg agcatattcc agagcagttt ccggatcatg
     4141 aatggaacaa ggaccacata ctaccaacag acgaggatcg cgcccggcga taatatctga
     4201 aatggtttta cgcgagtcag caatctgggc ttcctgttgc aggcttaatg gaaaagcggc
     4261 cttcagttgt tccggagtca ttaaaacttg ttcgtcggta atatgtacgt tattcagcgc
     4321 gtctttttgc atgatggcga tcctgtttat gctcgtttgc gatagttgat cctcagcgag
     4381 gatgacgtaa cgataacaca taaagtaaag ttttcaatcc atatttcgta catttttctt
     4441 tacacaagca atttattcg
//
LOCUS       DAZUCC010000297         4344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZUCC010000297 DAZUCC010000000
VERSION     DAZUCC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.299"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(2..343)
                     /gene="trbA"
                     /locus_tag="ACHY09_004746"
     CDS             complement(2..343)
                     /gene="trbA"
                     /locus_tag="ACHY09_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFS3887595.1"
                     /translation="MHTGDLCFFTGASLFIVGGVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCVLPDNFLFLCGAVMNQCAVPVVSMAVGTIPVITKFERFRRIFVCIMLSSAWSG
                     VMWFFIISLVK"
     gene            complement(357..1100)
                     /gene="traF"
                     /locus_tag="ACHY09_004747"
     CDS             complement(357..1100)
                     /gene="traF"
                     /locus_tag="ACHY09_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFS3887596.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIARLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(1093..1350)
                     /locus_tag="ACHY09_004748"
     CDS             complement(1093..1350)
                     /locus_tag="ACHY09_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFS3887597.1"
                     /translation="MKVIFTSNRLVDFLIRLVITAIVISPVIIWSWDTVKETTAGGVL
                     AAAFIILYSGVLLFILYGCFSALADLQKPDERKSDERNEDE"
     gene            complement(1364..3268)
                     /gene="traN"
                     /locus_tag="ACHY09_004749"
     CDS             complement(1364..3268)
                     /gene="traN"
                     /locus_tag="ACHY09_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFS3887598.1"
                     /translation="MKEKINTFFRSLNVNDSGRPRNICAIVFFLSFSVFADSNSDYRA
                     GSDFARQIQGQGIGSIQSFNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWA
                     TSETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEF
                     TNYTCERDTMVEEYCSRTARITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPV
                     AGTVESASLSAHAGLYFLNSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLT
                     GSGCTANGNCLPHGNGDRMVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLKGTECSEPGGTKTGVMEGKTWSITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGACLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKSPDCRGITVDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQ
                     IAGQLKQVGQ"
     gene            complement(3362..3742)
                     /locus_tag="ACHY09_004750"
     CDS             complement(3362..3742)
                     /locus_tag="ACHY09_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887599.1"
                     /translation="MSLPAGYYRIEPDIRALVAAMNVHGFRTYASCQGHGFPVTKLLP
                     YIAFACPVKMTALLEQRLRQDAESAIPRLTWGWSVKGAFNSDLQLCFRLQPEGPHCWY
                     HRYCRRSLRADFRTLILLLKSLSE"
     gene            complement(3739..>4344)
                     /gene="trbC"
                     /locus_tag="ACHY09_004751"
     CDS             complement(3739..>4344)
                     /gene="trbC"
                     /locus_tag="ACHY09_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFS3887600.1"
                     /translation="TAGLFLSAHASENVNTPENRQFLKQQETLSQQLREKPDTQLQAW
                     AEQQVRANPLQHADNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPEEGLKRMLGETR
                     HYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGIRSVPALVVFCS
                     QGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKLQ"
BASE COUNT         1048 a         1241 c          979 g         1076 t
ORIGIN      
        1 gtcatttcac taatgaaata ataaaaaacc acattactcc tgaccatgct gatgacaaca
       61 taatacagac aaatattctt ctgaacctct cgaacttcgt gataaccgga atggtaccga
      121 ctgccattga aacaacgggt acggcacact gattcataac cgcaccacaa agaaacaaga
      181 aattatctgg cagcacacat acacttatga catttactac aaagaagaca aacgagctca
      241 gcagataaac tcctttatta tttggtcctg taaagaaata accaacaccc cccacgataa
      301 acaggctggc acctgtaaaa aaacacaaat cccctgtatg catatatatc ctttggttaa
      361 aaattcggtt taaaatcttc agatacgttc aggaactgtt ttgccaggtc atcctgcgaa
      421 ataaagccgt atgataatgg acgaacactg ccctgtttcg ggtctaccag catcatggcc
      481 gggaaatatt tcacgccgag gcgctgcgcc tgcccctggt cagtccggga atccggcaat
      541 agtggattaa tcacgccatc cacggaaacg ggaataacac tcagaccata cgtatcccgg
      601 aagccattaa tgacctgcgc cagctgcccg tcgatggggt cctgcccccg gtaaaaaaac
      661 ataatgccgt aatgctctgc cagtctggca atcgcctgtc gctgctgcgc ctggtctgct
      721 gccagctggt tccgtaccgt gccgttgtaa tggctgtact gcaggttata gtccagttca
      781 ggatgtgcca gcatggcctt tttggcgctc atggtgaaaa gcccggcctg ctgagtccag
      841 taattctgca gccggaaata tttcacaaaa ttatccacgc cgggatacag aatggcttcg
      901 tacagcgccc gctttgttgc cgtctgcagc gcggtcagtt tctgcataat atccggctcc
      961 tgacggggag ctgctggtac tggtttattt tctttttcct tcggatttat tttctcgtta
     1021 taccactgcc agcctgcatc tttccctgac gccggaaaaa taaagcagca gagtaacagc
     1081 ggcagtaatg ctttattcat cttcattcct ttcatcactt tttctttcat caggtttctg
     1141 caggtcagcc agtgcagaaa aacaaccata cagaataaac aggagcacac cggagtaaag
     1201 gataatgaaa gcagcagcaa gcaccccgcc tgccgtggtt tcttttaccg tatcccacga
     1261 ccagataatt accggggaaa tgacaattgc tgtaataaca agccggataa gaaaatcaac
     1321 aagacgattc gacgtgaata tgactttcat tattttcccg accttactgc cccacctgtt
     1381 tcagctgacc ggcaatctgc tctttcactt tttcggtcag cgcaccatta tccgggattt
     1441 tctggttatt catcaggtct tcgtagaagt tagtgaagtc cagctggtcg aacttaatac
     1501 gctgcagttc atccacggtt attccccggc agtcaggact ttttgcgctg ccaaacccga
     1561 tacgcaactg cccgttacgc ccctgctgct ggactatctg cgccagcttc gaatcaaact
     1621 ggcagtaact gcgctttttc tgcagacaga cacccagcac tttccgggag cagaactcgc
     1681 ccacactcac cgtcagtttg ttgtcctttg cctttcccag cgccttttct tcgctgttgc
     1741 acttcgccaa acccacatcc tgaccccagc caccatcctt acagcagtta ctgaagccgg
     1801 ctgcggcctt acggcagaat ttcgcctcgc ccgtgaaagc cctgacatcc acaccattca
     1861 gggcggcaac atccttacct gcggcggcaa gcgcagccag ctgtgatact gcctcaccaa
     1921 aatcactgct ttttccgctc tgcgccttgt cgcattcacc gtcaaggcag aagacatcac
     1981 caccacagac catcactttt ccggacgttc tggactcgca ggagtacgtg gcatactcat
     2041 gcagacaagc tccctcatcg gaataaaacg cgcactgacg tgaggccagc gtacaggccg
     2101 gattatcgac atatttctga caggtgccgt tatctgcgga ctgtgtgaca tatttatccc
     2161 ggtatgccca gcacgcctgt gtgatgctcc aggttttacc ttccatcaca cccgttttcg
     2221 taccacccgg ttctgaacac tcagtgcctt tcagcacgcc atccgcttta ttaaacggac
     2281 aactctccac ccactccacc cgtggaaccc attccttatc acggaccttc atcctgagtt
     2341 tcagcgtaaa ggtggaagca ccactgacaa gcgattcata gaccatcctg tcaccattcc
     2401 cgtgcgggag gcaattaccg tttgcagtac agccactacc ggtcagaacc tgcccctgtg
     2461 tgacagaaaa cccggagggc acaggtatgg tgaaagtccc gctctgtcct gtaaccagtt
     2521 gcacattaaa ggctgtgttc ataaagtcgt aacgggagtt aaggaaatat agccctgcat
     2581 gagccgacag cgaggcactt tccactgtcc cggcaacagg tacggtaacg ctgaacacca
     2641 gtttcagacc gttcatactg aaccggaact gtgagtgcgg tatcgtcacc tcacgatatt
     2701 catccgtgta tttccagtct cctgtgatcc tggctgtcct ggaacaatat tcctccacca
     2761 tcgtgtcacg ttcacaggta tagtttgtga attcactgcg gtttatctgc tgcgcactgc
     2821 actgctgtcc ggtatttccc acgatactgt cagcccggtt caccacatcc ctgccggtct
     2881 gaataaacgg cgcatccggt gacagaatgt ctttcggctt gttcatgaat gactctgtga
     2941 tggttttgcc ggtttcactg gttgcccatt gtgtggtgcc gttatttttc agaccgccat
     3001 ccccgccggc agtcacccca ccgtaatact gcgtctcatc cgggttcgcg ttatacccgg
     3061 ggatactctc ctgcggatta aaactctgaa tactgccaat accctgcccc tggatctgac
     3121 gggcaaaatc ggaacctgcc cggtaatcac tgttgctgtc ggcaaataca gaaaagctca
     3181 ggaaaaaaac aattgcacaa atattgcgcg gtcttccgga gtcattaaca ttcagagacc
     3241 tgaaaaaggt atttatcttt tctttcataa cctgatccat actggagaat gaagtgatgt
     3301 catctcaact tcagaacggt gttgattttg gtgtatggag cttcctggcg taccgggatt
     3361 actactcaga taacgacttc agtaacaata tcagcgtcct gaagtctgca cgcagtgaac
     3421 gacgacagta ccggtgatac cagcaatgag gaccttccgg ctgaagccgg aaacagagct
     3481 gaaggtcact gttaaaagcc cccttcaccg accatcccca tgtcagacgt ggtattgctg
     3541 attcagcatc ctgccgcagt cgctgctcaa gaagggcagt catttttacc ggacaggcaa
     3601 aggcaatata aggcagcagc tttgtgaccg ggaaaccatg tccctgacag gaggcatatg
     3661 tacggaatcc gtgaacgttc atcgcggcca ccagtgcacg gatgtccggt tcaatccggt
     3721 aatacccggc aggtaaactc attgcagctt atccccggtg ccgtccagca gtccctgagc
     3781 cacctgccgg cagtctcccg tggcggccac tttttccagc gcctgcctga cgcgcagatt
     3841 tccccggatg atgtcgtgcc cctggctgca gaacaccacc agggccggca cgctgcggat
     3901 gccgtactgt gaaaacaacg tcgggtcgat ctgaacacca tcggtcgcgc catcttttac
     3961 cagggacaac acggcttcag cggtagtctt caggtcattg ttcaccatac cccgcagtgt
     4021 ggccggaatg ccgtagtgcc tggtttctcc cagcatccgt ttcagtccct cttcgggaat
     4081 ggagaacgac acaaaataca cggccccctg ctgtggtttg tctttctgag cagcctgctg
     4141 tttacggacc agttcatcga gaaactggtt atcggcatgc tgaaggggat tcgccctgac
     4201 ctgctgttct gcccatgcct gcagttgtgt gtccggcttt tcacgcaact gctgactcag
     4261 tgtttcctgc tgcttcagga actggcgatt ttcgggcgta ttcacatttt ccgaagcatg
     4321 ggcagaaaga aacaatcctg ctgt
//
LOCUS       DAZUCC010000298         4309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZUCC010000298 DAZUCC010000000
VERSION     DAZUCC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.300"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(178..1488)
                     /locus_tag="ACHY09_004752"
     CDS             complement(178..1488)
                     /locus_tag="ACHY09_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFS3887601.1"
                     /translation="MSELNQLQKWRREFHHIPESGWCEFITTSKIITILRGMGWQVIT
                     GNKYLSTEYIMGRNATEVADSIKRAVDSGVPHELLDEMDGFTGCVALWDTGKNGAVTA
                     FRFDIDCVQVSESKSKDHIPEKMGFASIHSGLMHACGHDGHISIGLGLAQWVSENNHK
                     LCGKIKLIFQAAEEGVRGAKPIAEGGILDDCDYFLSSHIGMGVPTGNVVASPTDLLCT
                     TKMDFFFYGTPAHAGVNPHLGNNALAAACHCATQLLAIPRHGEGMSRINIGTLNAGEG
                     RNIIPSFARMQLEVRGETETINQYMREHALKIAQGISSIFGLRFEYKVVGEATDLHND
                     PLLVNLIHYIAQEDMNMSLTEKSFGGSEDATLLIRRVQKIGGKAAYIILGSDLKAGHH
                     EVDFDFDENVLDTGVKLFSLCLERLNSPGLNKIVGNGKLESELY"
     gene            complement(1501..2772)
                     /gene="dcuC"
                     /locus_tag="ACHY09_004753"
     CDS             complement(1501..2772)
                     /gene="dcuC"
                     /locus_tag="ACHY09_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HFS3887602.1"
                     /translation="MFNVITGCVILIITVVLLIKRYDTRTTLIGAGILLCIVAMSPLN
                     AFSAFASRMTESGLIQAICTSMGFACVMKFTGCDTCLVHFLTKGLSRLGIFLIPAAVT
                     VSFIVCIAIPSAAGASAAVGATLIPLLISARIHPAIAGAAILSGTIGAYLNPGVAHNS
                     FISKISQTPLMEFVVFHTPTTITCGVICAVILTVVAIVRKETKCDSAGNISEQTQVEF
                     TDRDICFYLKSVAPFLPVALLMLAEFGFLGETKITVPAAMILGCLYALIVSKSNPKEL
                     VSSFFEGMGTSYANILGIIISAAVFIEGLRSVGIIDVFIRFLTDSPEFARWGGTLGPF
                     LMGLITGTGDATAFAFNEVVTPHANNFGYDTNTLGAAVMLASALGRNMSPLAGATIIC
                     AGLAGVNPVEIAKRTAPGMIVTVLFIALLFL"
     gene            2908..3825
                     /locus_tag="ACHY09_004754"
     CDS             2908..3825
                     /locus_tag="ACHY09_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3887603.1"
                     /translation="MKYMPKLQQIKFFNEIVLCGSLRAAARKMNISQPALSRTIKELE
                     NVLGCNLLIRTRDGIVLTEAGKIFSVHSRMILEEIETAALEVAKVNIDNTGKVAFGIS
                     SLFGVTILNNVLDNFRMKYPKKRVMIREGQLSSLIPFLREGVLDFAMGTLTKDMPVGD
                     FIVVELFNAPFTIVARKHHPLRNCTSIDELATARWVFPETQMGYYHYISRLIPFGHPD
                     EPYAPIFTDSVVCIMNLVLYDDYITVLSRARLQEARFKEILCSIPVKYQLPVSQYGLI
                     YPRKRLMTAPAQIMIEYFQQEIQRYDWNG"
BASE COUNT         1342 a          924 c          830 g         1213 t
ORIGIN      
        1 atcctcttcc catatcacca gtatatgctg agccgtgttt gtctatttgg ttaaaattcg
       61 ctacaatcct attctctctt ttgggaatat aaaagtctcc attttctctt tattcgttaa
      121 tggagactgt atttaaaggc agaaatcagg agtgattgac aggatacctg tcatcaatca
      181 atacaattca gattccagtt ttccgttccc tacaatttta tttagtccgg gagagttcaa
      241 tctctccaga cataacgaaa acagtttaac gcctgtatcc agcacattct catcaaaatc
      301 aaaatcaact tcgtgatgac cagcttttaa atcactgcca agtattatat atgctgcttt
      361 tcctcctatc ttctgtacac ggcgaataag gagcgttgca tcttcactgc ccccaaatga
      421 tttctctgtc aaagacatgt tcatatcttc ttgtgctata tagtgtatca gattgacaag
      481 taaagggtca ttatgtaaat ccgttgcttc accaaccact ttgtactcaa aacgtaaacc
      541 aaatatggaa gaaatacctt gtgctatttt cagagcatgt tcacgcatgt attgatttat
      601 tgtttccgtt tctccacgaa cttcaagttg catccttgca aaagacggga ttatgttcct
      661 tccttcacct gcgtttagcg tgccaatatt tatccgggac ataccttcac catggcgtgg
      721 aatggcaagt aattgcgttg cacagtgaca agcagctgcc agagcattat ttcccaggtg
      781 aggatttaca ccagcatgtg ccggagtacc atagaaaaag aaatccattt ttgtcgtaca
      841 caacaaatct gtaggtgaag cgacaacatt tcctgtagga actcccatac caatatgtga
      901 agaaaggaag tagtcgcagt catcaagaat accaccttct gcaataggtt ttgcaccgcg
      961 aacgccttct tctgcagcct gaaaaattag cttaattttc ccgcataact tatggttatt
     1021 ttcagatacc cattgtgcca atcctaaacc aatcgatata tgaccatcat gtccacatgc
     1081 atgcattaag ccagagtgta tggatgcaaa tcccattttt tcaggaatat gatctttcga
     1141 ttttgattca cttacctgta cacaatcaat gtcaaaacga aacgcagtga cagctccatt
     1201 ttttcctgta tcccataaag caacacaccc tgtaaagcca tccatttcat ccagcaattc
     1261 atggggaacg cctgaatcaa ctgcacgttt tatactatct gcaacttcag ttgcattacg
     1321 gcccataata tactctgttg acaggtattt attccccgta ataacctgcc atcccatacc
     1381 acgcagaata gtaattatct ttgacgttgt gataaattcg caccacccac tttccggaat
     1441 atggtgaaat tctctccgcc atttttgtaa ctgattcagt tcagacatat acgcctcctg
     1501 ttaaaggaat aataatgcaa taaacaatac cgtcacaatc attccggggg cagtgcgttt
     1561 tgcaatttca actggattaa ctccagccag accggcacag ataatagttg caccagccaa
     1621 cggagacatg tttctcccta gcgcagaagc cagcataact gcagcaccca gagtatttgt
     1681 atcgtaacca aaattgttcg catgtggtgt gacaacttcg ttgaatgcga atgcagtcgc
     1741 atcccctgtg ccggttataa gtcccatcag gaatggcccc aatgtaccgc cccagcgggc
     1801 aaattctggt gagtcagtaa gaaatctgat aaacacatca atgataccaa cagatcttaa
     1861 accttcaatg aaaacagctg cggaaattat gatcccaaga atatttgcat atgatgtccc
     1921 cataccctca aagaagctgg aaactaattc tttgggattg gatttactga caatcagggc
     1981 ataaagacaa ccgagaatca ttgcggctgg aacggttatt ttagtctctc caagaaaacc
     2041 aaattcagcc agcataagta atgctacggg aagaaatggt gctacagatt tcagataaaa
     2101 acagatatca cggtcagtga actcaacctg tgtttgttct gatatgtttc ctgcagaatc
     2161 acattttgtt tcttttctta caatagcaac aactgtaaga ataacagcac atattacacc
     2221 acatgttatt gtagtcggag tatggaatac gacgaattcc attaatggtg tttgtgatat
     2281 tttagatata aatgaattat gtgcaacacc gggattgagg taagcaccga tagttcctga
     2341 aaggatagct gctccggcta ttgcaggatg aatacgtgct gaaataagta atggtatcag
     2401 cgttgcacca acagcagctg atgctccagc agcagatgga atcgcgatgc aaacaataaa
     2461 actgacggta accgcagcag gaataagaaa gataccaaga cgagataatc ctttcgttag
     2521 aaaatgaacc aggcatgtat cacaaccagt gaatttcata acacaggcaa agcccatact
     2581 ggtacaaatg gcctgaatga gaccggactc agtcattctg cttgcaaatg ccgaaaatgc
     2641 gtttaaaggt gacatggcaa ctatacatag cagaatacct gcaccaatta gtgtcgttcg
     2701 tgtgtcgtat ctttttatca aaagcacaac tgtgattatc agaatcacac atccagttat
     2761 cacgttaaac atgagatcct ccttcttcaa agaaggataa tctttacttt atgtgatttg
     2821 ctcaaatatt gatcatgaac aaaaaaatca attaaccggg gatactatta tgatggaggt
     2881 gataacagaa aattatcagg cgaaaaaatg aaatatatgc ctaaacttca acaaattaaa
     2941 tttttcaatg aaatagtcct gtgcggaagt ttaagagctg ctgcccgtaa aatgaatata
     3001 tctcagccag cattaagcag aacgataaaa gaactggaga atgtattagg atgtaacctt
     3061 ctgattcgaa caagggatgg tatcgtttta acagaagctg gaaagatatt ctcggtccat
     3121 tcacgtatga ttcttgaaga gattgaaact gcagctcttg aagttgcgaa agtaaatata
     3181 gataacacag gaaaagtagc ttttggaatt tcttcgctgt ttggcgtaac aatactgaat
     3241 aatgtacttg ataacttccg gatgaaatat ccgaaaaagc gtgtgatgat aagagaggga
     3301 cagttgtcct cccttatccc tttcctgaga gaaggtgttc tggattttgc aatgggtaca
     3361 ttaacaaaag acatgccagt tggagatttt attgtggttg aattgtttaa cgcaccgttc
     3421 actatagtgg caagaaaaca ccatccttta aggaactgta catcaatcga tgaacttgca
     3481 actgcaagat gggtattccc ggaaactcaa atgggatact atcattacat atcccgtctt
     3541 attccctttg gacatccgga tgaaccatat gctccaatat ttaccgattc ggtagtttgt
     3601 attatgaatc ttgttctcta tgatgactat ataacggttc tctccagagc aaggttacag
     3661 gaagcaagat tcaaagaaat attgtgttca attcctgtta agtatcaact acccgttagc
     3721 cagtacgggc ttatttatcc acgaaaaagg ctaatgacgg caccagcaca gataatgatt
     3781 gaatattttc aacaagagat acaacgatat gactggaatg gatgagcaat acccggaatg
     3841 atggtgtaca ccgggataac aatttattcg gatgtgccaa tcccacggtt gtaatgggca
     3901 atataattgt tagccgattc gctaatggca gggaaaagcg cggcataaat ataagcccac
     3961 acacatgtaa cgtactgcag atactcgctt ggcagagttg ttacttcttg ggaggcagta
     4021 ccagccatca gatattcccg ttccatgcca aaacctgagt tacccggata ccattacatt
     4081 gcctgtattt cattcatctg ccacacacgt gtcagctctt tgtgacctga cgcaatcatc
     4141 tgactcagca gcgattcacg gtactggtct ttttggtgac cgttctgaca gagcaataca
     4201 gtgggacaaa cgaccatcgt catcactgga gaggcgtgca tggaaactcg tcaacaactc
     4261 aagttcgccg gaacggaacg ttaacaatag aactttactg tataaattt
//
LOCUS       DAZUCC010000299         4265 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZUCC010000299 DAZUCC010000000
VERSION     DAZUCC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.301"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(138..1028)
                     /gene="metF"
                     /locus_tag="ACHY09_004755"
     CDS             complement(138..1028)
                     /gene="metF"
                     /locus_tag="ACHY09_004755"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFS3887604.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(1410..2963)
                     /locus_tag="ACHY09_004756"
     CDS             complement(1410..2963)
                     /locus_tag="ACHY09_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS3887605.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3096..4220
                     /locus_tag="ACHY09_004757"
     CDS             3096..4220
                     /locus_tag="ACHY09_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFS3887606.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTWASEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGACLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
BASE COUNT         1108 a         1072 c          971 g         1114 t
ORIGIN      
        1 ttagatttgt atccttcaag taatgaggga gaaaacaagg ctgataacct tattttcccg
       61 ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact gtgtaatttt
      121 cacaaaaggc acactattta taaaccaggt cgaacaccca gtgtatggca aatcgcgtaa
      181 ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc acggcttaaa
      241 atcttcacca tatccatggc aatattcgcg cccaccagct tgcgggtttc agcatcgtca
      301 tccaggccgt cgaacatttg tgccatccac gccggaatac gcacgttggt catgtcggca
      361 aatttcttcg cctgtttaaa gttcgaaacc ggcaaaattc ccggaatgat ttccacatca
      421 atgcctgccg agacgcagcg atcgcgaaaa cgcaggtagc tttcgacatc gaagaagaac
      481 tgagtaatgg cgcggttggc tccggcatcc acttttcgtc tcaggttgag taaatcggcc
      541 tgggcacttt tcgcttctgg atgtacttcc ggatacgccg ccacggagat atcgaaatct
      601 gccacttctt ttaacagtgt caccaggtca gaagcataca tttctggctt accacttccc
      661 ggcggcaggt cgccacgcag cgccacgata tggcgaatac cgttattcca gtagtcgcgt
      721 gcaatggtcc gcagctcggc aggcgtcgca tcaatacagg taagatgcgg tgccgcttcc
      781 aggccggtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg ctcgccggag
      841 tttgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg atcgatggag
      901 ttccacaggg tctgctccat ttcactggta cgcggcggga aaaattcgaa cgaaacgtta
      961 atctgcccct ggacttctgc cagactctga ttcagggcat cccgctggct ggcgtgaaaa
     1021 aagctcatac cttacctcat caatcgctta tcgttatatg ttgtgttgtg cacatctatc
     1081 cgtttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac cgaaaaataa
     1141 ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaaac tcatcttaat tagaggcgat
     1201 aaaagatttc tctatagcac aatccacctc gcacgttttg actcatattc tgacaacaca
     1261 gcgacacctg ggctaattcg aaaaacaacg tcctgccacc ggatggtgat gctaaggcat
     1321 ctgattcggc ctgcgccctc gcgtaggccg aatacaggat ctgcgcaatc aacgggtaac
     1381 aaacagttga gtcaattcag tgcgcacttt tatttgatat ccttcacgcg cataccgttg
     1441 agctgttcat ccgtaatggt gttgcccgct ttgaagtaat caaccaccgc gtgataaacg
     1501 taataaccgc ccgttgtgtt acgcgctttc ccttcggtaa aggcggtaaa accatctcca
     1561 ccatcagcaa ggaaactctg agtagcaatg tgataaaccg tcgcatcttc aatgggtttg
     1621 ccattcagag tcagtgtgat tacccgctga ccaaccggct tgctgctgtc gtacttcatt
     1681 tccaggcctt tcgatacctg taaaacgcca ttgcttaaac ttgcgccgtg ttccatcaga
     1741 ctgcgtaatt gtttacccgt gagttccatc gtcaccagtt cgttggggaa cgggaacgta
     1801 ctgatgacgc cgcccatcgt gatcgcccct gcggggatct cattgcgaat gccgccagag
     1861 ttggttaacg ccaattgggt gtttttaccc gccgctacca gtaaagcgtc agccgccagg
     1921 ttcccgaggg aagccgattc accataggca cgttttaatt caaccggcga ttgcgccacc
     1981 gtttgttgca ccacttcatc aagcttttta ttccagccgt cgatcacctg tttcgtttgt
     2041 ggatcgggct tccactcgtc agcgtaaatg gttttaagct cgaagttttt caccgtaaaa
     2101 ttatgcggct tctctttgta gtcgagaacc agtttgccca catcaatccc gccgctgtca
     2161 gtggagagga tgagcgtatt gccgacttta atcggttccg gcgtacccac atgtgcatgt
     2221 ccggtgatca aaatatccag ccctttcacc tgacttgccg tctgaatatc tttatccagc
     2281 gcgcgacgca catctgtgcc tcccatactg gactggcggg ccggaacacc ttcatggatc
     2341 agggcgacgg tcaaatcaac cttgccttta agttcatcga tataacgttg tagccattta
     2401 atttcatcac gcgcctcaat acccactcgc gtggcagcag atactgtgtc attaaaggca
     2461 aatacaccgt gcaaacctat aacgcctatt ttcacaccgt ctttttcgat gatggtatag
     2521 ggcttatccc agaatgattt actgctgttc tgataaaaaa cattgccctg cacaatcggg
     2581 aattttgcct gactcaactg taataatgtg ttgtcccagc cgtggtcaaa ttcatggttg
     2641 cctatcgtga cggcatcaaa cggcatagta ttcataatat cgataatagc cttacctttg
     2701 gtcaggctgc tgatatatgg cccggtaaaa tagtcgcccg catcaaaaaa ccatgtcgct
     2761 ttattttttg ctttttcttg cttaaccagc gtagtgatat ttgcccagcc gccaatatcc
     2821 cgtttaccgt cagcgatcca cggcacttta taaggttcta catgcgcatg gaggtcgttg
     2881 gtataaataa tagtgacatc tttagcgcaa gcccagaacg gcagtgttaa tgcgataccc
     2941 gcagcaagta ttttaatttt cataaacaat ccctttcagt gagataaagg attgctacat
     3001 tacgttctga aagacttatt tatgtgagag taatcacatc tgcaggggat agttgccgga
     3061 caaaatcaac gaccttgtga aatcgttggt gatatatgtc gactctgttt acgattaatg
     3121 cctgtaaaac gttcggttgc cgaaatttgg gattggcctc atcggaagat tacagttggc
     3181 cagactataa actgggttat ccggcactgc actgtcgggc atgtggcagt tacccgccgt
     3241 tgtttgacga gcaacaattt cgtgactggt tgtcagttca cttatctacg tgggcctcgg
     3301 agaagggtca tttctgcccg gtatgttatg gcacagacat gatttgttat ggtcacaatc
     3361 cacagggttc gcaacgcatt caatgccgta actgcaaaaa agtctggacg cctaaacagt
     3421 atcagaaaga aattacgcat ccccaggcca ttgaaaccgt tcaactgttc ataccctttc
     3481 aaggggccag cgcagtacaa aaactgtatg ttttagtcag tcttgacgcc actcgtggca
     3541 acattcttca tctctccacg aattatacgc aacatcagac aggagacagt ctgcggtaca
     3601 gttacaaggg caacacagaa ccaactatgc accatcgcga tatcgtgcag aaggtagata
     3661 tgcgtgaagc gcaattcttg cgccggagcc agttcgatga aattcagtat ggcagtgctg
     3721 tactcaagcg caacggtaag ggagccatac tacgcccggt tatcacggca cacgggcatt
     3781 tcagaatact gaaaatccgc tatccagatg tcaaaacgca tattatttca cacgaatgtt
     3841 ttctaagagg cgcaattatt acagcctggg cagatcagtt ccgccaacaa caaggtgaac
     3901 tttggttcgt agaagaagaa atcagtgaca gtaacgctga tacgccctgg cattttaagg
     3961 gaacgacata ccatggttgg tggcaaaatc agtggcagcg ctgggagcag gggaataact
     4021 gcaagatggt ctgcctactc acgggagcct gcttagaaag gggcgcaaat gtttctctgg
     4081 caaccagtcg ttgttttatc acatggctga cagaccaaca cgactttacc caaagcgcgt
     4141 tattatccgc aggtcgcgta acacaaatgc tgacctcact ggcgttaaaa tacaatgaat
     4201 cgctcactcc atcttgttag tcaatacact cgacaaacga ccataaatta agagagaaca
     4261 ctacc
//
LOCUS       DAZUCC010000300         4241 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZUCC010000300 DAZUCC010000000
VERSION     DAZUCC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.302"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..358
                     /gene="traA"
                     /locus_tag="ACHY09_004758"
     CDS             5..358
                     /gene="traA"
                     /locus_tag="ACHY09_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFS3887607.1"
                     /translation="MNTSVSVPGAFAPVKKKSSFFSKLNLLRLVRAVIPAAVLMMFFP
                     QMALAAGGQDLMQSGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLAGFA
                     IISVFIAVGMTVVGL"
     gene            373..684
                     /gene="traL"
                     /locus_tag="ACHY09_004759"
     CDS             373..684
                     /gene="traL"
                     /locus_tag="ACHY09_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFS3887608.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGIET
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            706..1272
                     /gene="traE"
                     /locus_tag="ACHY09_004760"
     CDS             706..1272
                     /gene="traE"
                     /locus_tag="ACHY09_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS3887609.1"
                     /translation="MEHGARLSTSRVLAIAFIFMSLLIVLSLTVNVIQGVNNYRLQNE
                     QRTAVTPMGFNMPFAVSQNSADASFLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNKMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            1259..1987
                     /gene="traK"
                     /locus_tag="ACHY09_004761"
     CDS             1259..1987
                     /gene="traK"
                     /locus_tag="ACHY09_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFS3887610.1"
                     /translation="MRKNKTVIISACLLFSGSVLAGNGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGETAGAWETSTPYESLLVSISQAVRAGKMPEGWYQI
                     PVTKETLQAPAGMSAKAEAVWTGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMF
                     SQPVSQLLAAARMDVYVIRDGEGN"
     gene            1987..3417
                     /gene="traB"
                     /locus_tag="ACHY09_004762"
     CDS             1987..3417
                     /gene="traB"
                     /locus_tag="ACHY09_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFS3887611.1"
                     /translation="MASINTIVKRKQFLWLGIVIVGSVSAIGGALYLSDMDMSGNTDA
                     VAGQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRREMDVLNKQRGDD
                     QRRIEKLGQENAALAEQVKALGANPVTVTGEPVPQMPASPPGPEGEPQPGNAPVAFPP
                     QGAGAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKTFSYDAGTKQGPSLPYIPSG
                     SFAKAILIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVS
                     SERAIVRTRNISCLKEGKTIDMPVKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVD
                     GIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVI
                     PIGAGNEVTVVFQDGFQLKTVEEMALEQAQGQAEESNPESPVPNPPSAESHLNGFNTD
                     QMLKQLGDLNPQQFMSGGQEGGNDGK"
     gene            3407..3994
                     /gene="traP"
                     /locus_tag="ACHY09_004763"
     CDS             3407..3994
                     /gene="traP"
                     /locus_tag="ACHY09_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFS3887612.1"
                     /translation="MANNTSSWQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEINFVRQTATAGQFPVSECRFSSSDLPGAEEKEICHYRNAG
                     ATEYARETDRSLMQLVTAFWATLALMYMSFAAGTGKYPMWPGKMKCVRVGTADERLRE
                     VLAEDASLPGKISKCPVYFPDDRTNRNNGDKNEHA"
     gene            3987..>4241
                     /locus_tag="ACHY09_004764"
     CDS             3987..>4241
                     /locus_tag="ACHY09_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFS3887613.1"
                     /translation="MRNIHVITARSVPDKSVSDDFMHAVLSNCATRIVLPAPKEFSSE
                     SLPHNFNMAAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKSV"
BASE COUNT         1014 a         1001 c         1216 g         1010 t
ORIGIN      
        1 attgatgaat acttctgtta gtgttccggg ggcttttgcg cctgttaaaa agaaatcatc
       61 ttttttctct aaactgaacc tgcttcgcct ggttcgtgct gtgatcccgg ctgctgtgct
      121 gatgatgttc ttccctcaga tggctctggc tgctggcggt caggacctca tgcagtccgg
      181 gaatgatacc gtcaaagcca cctttggtaa ggactccagt attgttaaat gggtcgttct
      241 ggctgaagtg ctggtcgggg cggtcatgta catgatgacc aaaaacgtca agttcctggc
      301 cggttttgcc atcatctctg tatttattgc tgtgggtatg accgtcgttg gtctctgaca
      361 ggaaataaaa cgatgtcggg agacgagaat aaacttaaga aatatcgttt cccggaaaca
      421 ctgaccaacc agagccgctg gtttggcctg ccactggatg aactgatccc cgcagcgatc
      481 tgtattggct ggggtatcga aacatcgaaa tatctgttcg gtattggtgc agcggttctg
      541 gtttatttcg ggattaaaaa actgaaaaaa gggcggggca gctcctggtt acgggatctt
      601 atttactggt atatgccaac agtcctgctg cgcggtattt ttcataatgt tcctgattcg
      661 tgtttccggc agtggattaa atagaactga tacctggatt gttatatgga acacggtgcc
      721 cgcttaagta ccagtcgtgt actggccatt gcttttatat ttatgtccct gctcattgtt
      781 ctcagtctga ctgttaacgt gattcagggg gtgaataatt accgccttca gaatgaacag
      841 cgtacagccg tgacaccgat gggatttaat atgccctttg cggtatcaca gaacagtgct
      901 gatgcctctt ttttgcagca gatggcgctg gcgtttattg ccctgcgcct gaatgtctca
      961 ccggaaatcg tcgatgcttc tcaccaggca cttctgcagt atatccgtcc tggcgcgcag
     1021 aataagatga aagtgattct ggccgaagaa gcaaagctca tcaaaaaaga taacgtgaat
     1081 tctgcttttt tccagaccag tgttcgtgtc tggccacaat atggtcgtgt ggaaatccgt
     1141 gggattcgga aaacatggat tggtaactcc gaacctttca ctgatatcaa acattacatt
     1201 cttattctga aacgggaaaa cggggtgacc tggctggata atttcgggga aacagacgat
     1261 gagaaaaaat aagacagtaa taatatccgc ctgcctgctt ttttccggca gcgtgctggc
     1321 aggaaacggc acactggcac ctacagtggt tccgatggtc aatggcggtc aggcaagtat
     1381 tgctatcagt aataccagcc cgaatctgtt taccgtcccc ggagaccgga ttatcgccgt
     1441 gaacagtctg gatggtgcgc taaccaataa tgagcagact gcatccggtg gtgtggtggt
     1501 ggccaccgtc aacaaaaagc cttttacgtt cattctggaa acagaacgtg gtcttaatct
     1561 ttccattcag gccgttcccc gtgaaggagc cgggcgtacc attcagctgg tcagcgattt
     1621 gcgcggaaca ggagaaactg ccggtgcatg ggaaacatcc acgccttacg aatccctgct
     1681 ggtttccatc agtcaggcgg tccgtgccgg aaaaatgcca gaaggctggt atcagatccc
     1741 cgtgacaaag gaaaccctgc aggcccctgc cggaatgtcc gcaaaagctg aagccgtatg
     1801 gacggggaat cacctgaaga tggtgcgctt taccgtggaa aataaaacgc tgtctgcgct
     1861 gaatatccgg gaaagtgact tctggcagcc gggaacacgg gcggtgatgt tcagccagcc
     1921 tgtcagccag ttactggctg ctgcccgtat ggatgtgtac gtcattcgtg acggggaggg
     1981 caactgatgg ccagtatcaa caccattgtg aaacgcaagc agttcctgtg gctggggatt
     2041 gtgattgtcg gatcggtatc agcaattggt ggtgcgcttt atctgtctga tatggatatg
     2101 tccgggaaca ctgatgccgt tgcagggcag gagcctgtgc cggatatgac tggtgttgtg
     2161 gataccacct ttgatgacaa ggtgcgtcag catgccacga cagagatgca ggtgacggcg
     2221 gcacagatgc agaagcagta tgaggaaatc cgtcgtgaga tggatgttct gaacaaacag
     2281 cgtggtgatg accagcgtcg cattgaaaag ctggggcagg agaatgctgc gctggcagag
     2341 caggtgaaag ccctgggcgc gaatcccgtt acagtgaccg gagagccagt tccacagatg
     2401 ccggcatccc ctcccggtcc ggagggggag cctcagcccg gtaatgcgcc cgttgctttt
     2461 ccgccgcagg gggcaggtgc agtgccaccg cccaccgcgt tctatccggg caacggagtc
     2521 acgccgccgc cacaggtgac gtaccagtct gtgccggtgc cgaaccggat acagcgtaaa
     2581 acgttcagct atgatgctgg cacaaagcaa ggtccgtccc tgccgtacat tccgtcaggc
     2641 agttttgcga aagccattct gattgaaggg gcggatgcca atgcctctgt caccggtaat
     2701 gaatccacgg tgccgatgca gctgcgtatc accggtctgg tggaaatgcc gaacagcaaa
     2761 acctatgacg caacgggatg ctttgttggg ctggaagcct ggggggatgt gtccagtgag
     2821 cgtgccatcg tgcgtacccg caatatcagt tgcctgaagg agggtaaaac catcgatatg
     2881 ccggttaaag ggcatgtcag cttccggggt aaaaacggta tcaagggtga agtggtgatg
     2941 cgtaacggca aaatcctcgg ctgggcatgg ggcgcgggat ttgttgacgg tatcggtcag
     3001 ggaatggagc gagcttcaca gccagctgtc gggctgggag ccactgccgc ttacggggcc
     3061 ggcgatgtcc tgaaaatggg tatcggtggt ggtgcatcaa aagcggcaca gacactcagt
     3121 gactactaca tcaaacgcgc tgagcagtat cacccggtga tacccatcgg ggcgggtaat
     3181 gaagtgaccg tggtgttcca ggatggtttc cagctgaaaa ccgtggaaga gatggcgctg
     3241 gagcaggcac aggggcaggc ggaagagagt aacccggaaa gccctgtgcc gaatcctccc
     3301 tcggcagaaa gccacctgaa cggttttaac accgaccaga tgctgaagca gctgggcgac
     3361 ctgaatccgc agcagtttat gtccggtggt caggaagggg gcaacgatgg caaataatac
     3421 gtcgtcctgg caggcgtgcc atgccgcccg gtatgttgtc gcccgggttc tgcgtggact
     3481 gttctggtgt ctgaaataca ccgtcattct gccactggcc acaatggccc tgatggcgtt
     3541 atttgtttta tggaaggaca acaccacgcc ggggaaatta ctggttcagg agattaactt
     3601 tgtaagacag acagccactg ccgggcagtt tccggtcagt gaatgccggt tttcttcctc
     3661 agaccttccc ggagcagaag aaaaagaaat ctgccattac cgcaatgctg gagccacaga
     3721 gtatgccagg gagactgacc ggtcactgat gcaactggtc accgcattct gggcaacgct
     3781 ggcacttatg tatatgtcgt ttgcggcagg caccgggaaa tatccgatgt ggcccggaaa
     3841 aatgaaatgt gtccgggtgg gaaccgcaga tgaacgactg agggaagtac ttgctgaaga
     3901 tgcttccctg ccgggaaaaa tcagtaaatg ccctgtttat tttcctgatg acagaacaaa
     3961 cagaaataac ggagataaaa atgaacatgc gtaatattca tgttattact gcccgctctg
     4021 taccagataa aagcgtatca gatgatttta tgcatgctgt tctcagtaac tgcgccacaa
     4081 gaatcgtact tcctgcaccg aaggagttca gttctgaatc attgccgcac aattttaata
     4141 tggctgctgt cggtgtgatg aagaagggta tgtcgatgag cttattttct tctccggggc
     4201 atattgtggt tgccggacaa tccggtggcg gaaaatctgt t
//
LOCUS       DAZUCC010000301         4195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZUCC010000301 DAZUCC010000000
VERSION     DAZUCC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.303"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1343)
                     /gene="emrY"
                     /locus_tag="ACHY09_004765"
     CDS             complement(<1..1343)
                     /gene="emrY"
                     /locus_tag="ACHY09_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFS3887614.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVF"
     gene            complement(1343..2506)
                     /gene="emrK"
                     /locus_tag="ACHY09_004766"
     CDS             complement(1343..2506)
                     /gene="emrK"
                     /locus_tag="ACHY09_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFS3887615.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTTPIEKEISNIISHNGQL"
     gene            2922..3536
                     /gene="evgA"
                     /locus_tag="ACHY09_004767"
     CDS             2922..3536
                     /gene="evgA"
                     /locus_tag="ACHY09_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFS3887616.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            3541..>4195
                     /locus_tag="ACHY09_004768"
     CDS             3541..>4195
                     /locus_tag="ACHY09_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3887617.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     "
BASE COUNT         1290 a          880 c          825 g         1200 t
ORIGIN      
        1 ttaaatacgg ggttaaattg atcgatggtt gctgtcaact gactatggtg taacgattcg
       61 cgtcgtcccc acagcgtcat tgtcaacgac gtaccaactg atcctgacaa ggtacgaaaa
      121 aaattactca tactcgaggc attggcaaat ttattatctg gcaaccctga aaacgaaatc
      181 gttgttaagg gtaaaaagaa acaggcaacg gcgaatccct ggaaaaactg tggcaaaatg
      241 atgcctgtaa aatcaatcgt tggcataaat gtcacagaac gccagtaata gcaaaccgca
      301 tacatcaaaa aactaaatgt caccaacaac cgcatatcaa ttttgttgcc ataacgtcct
      361 atcaaaggtg atattaatag cggcatgatg ccgatgggcg cataggcaag tccggcccat
      421 atcgcattat accccatcgt ttcctggagt aactgcggca taaggactat cgccccagag
      481 taaaataaat acgcgcatgt gatactcaca ataccaatgg tgaaattacg ggacttaaac
      541 aaactgagat caagaatcgg attctctgag gtcgactccc aaatgactaa agaaatcaga
      601 gaaataactg acactactgt tagtattatt attgtactcg agttgaacca atccagatcg
      661 cgccctttat caagcattat ctgcaagcca ccaacaccga gcactaacag ggtcagtcct
      721 ggtagattca ttttgaccgg tgaagtctca gtttctcttc ctttaagtaa ggttaagcat
      781 aatgtcagaa cgataatccc catagggaca ttgattaaaa atatccaacc ccagctaaag
      841 ttatcgcaaa tatagccgcc caatatcggc ccacatatcg gagcgataat tacggtcatt
      901 gaccataatg ccaaagcaaa tgttcttttt tctggcggat aattccttaa taataaactc
      961 tgtgacagtg gaattaacgg ccccgccatt aagccctgaa cgactctaaa aaatatcagc
     1021 acatcaagat tggtcgatag gctacacatt aatgaagata gtgaaaaaaa ggttactgaa
     1081 agtaaaaata atcttaattc gcctattctt tgtgccagcc tgccggtaac agggatcgca
     1141 atggcatttg ctacgccaaa cgaagtgata acccaggtgc cttcgtctgt tgatgctccc
     1201 agaaagccag atattgtcgg tattgcgacg ttagaaatag tggaatccaa catttgcata
     1261 aacgtcgcta aggacaatgc aatagtaacg caccataacg tcccaccggt taatggtgcc
     1321 ggagttgatt tagtgattgc cattaaagtt gtccattatg cgaaataatg ttgctaattt
     1381 ctttttctat cggagtagta tcgataacta aagccttact ggtataagca ggcatggagg
     1441 tcacggttga agccagctca ggcatctcag caatgtcttc gtccttcgta tcaatagttg
     1501 cagtcatcga taaaccaata cgtaagggat gttccatgag ttcttttgga tcaagagaaa
     1561 cttcaaccgg tacacgctga acgattttga tccagttccc tgtcgcattt tgtgcaggta
     1621 ataaggagaa cgcattgccg gttcccatat tgattcctgt cacccgacca tgaaacacaa
     1681 cattttcacc ataaagatcg ctgataatat tgaccgattg accaatccgt acatccgtga
     1741 gttgtgtttc tttaaagttg gcattaaccc acatttgacg tgccggtacg acagccatta
     1801 acgattgtcc gggactcact gtttcgccga cctgaacact tcgctgggca atatagccgg
     1861 taacaggact cttaatatcc gtacgtttaa gcgctaacca ggcttctttg gttgcatctg
     1921 ccgcttcaac gacttgtggc tgacggttta atggtgtgtt cattactaaa gctttattcg
     1981 ctttataagc ctggatagcg gcattcaatg ccgctttgct acttattaac gtatctttgg
     2041 tatgctccag cgtttctttt gaaataaccc cctgcttcgc taaaggcaca cgacggttat
     2101 aatcttctaa agattgttga tactgaatac gtgctgaagc gacttcggca ctgtattgtt
     2161 tatcctgtaa gtacagttta ttcgtttgcc gaacaatatt tgccagatta tttttagctt
     2221 tattgagtgc gatagtggca tcagtcttat ccagtgagac taaaatgtca ccctgtcgaa
     2281 cgtagttcgt atctttatga ttaacgacag tgacgctgcc tgaaacttgt gcagaaattg
     2341 gatctgcatt ccccgtgaca taggcgtcat ctgtactaat catgtcttct aattccattg
     2401 accaataggc ataggcacct gaaaacgcaa taaataaaac taccgccaat aaagaaaagt
     2461 attttcttct gttagaatgt tttttatttg aattaatctg ttccactttt atctctcatt
     2521 tcgcatagat gaaatttatg aattgaatga aaagagtatt cattcttcat atcaaacaca
     2581 gtgcatgtgt tgcacattat cccaggaacg ttggaggcct aatttaaatg aggtgcaaaa
     2641 aaaacaacag aggactaaac cgtggctttt gcaatacaat tcttacgcat gtaggattag
     2701 tgagaagact tatagtgcta atgtgaaact ataaatcatc ggtacaatcc ctgattttat
     2761 tgttgacatt tcatttatgc cgactattta tatggtatac ttgttgaatt atcttaaagg
     2821 aagctcaaat tttcttattt tgattgagaa aatgagatga tgccttatgt ctgtattact
     2881 acagggagaa gggaaatgct tcattgcaaa gggaataatc tatgaacgca ataattattg
     2941 atgaccatcc tcttgctatc gcagcaattc gtaatttatt gatcaaaaac gatattgaaa
     3001 tcttagcgga gttgactgaa gggggaagtg ccgttcagcg ggtggaaaca cttaaacctg
     3061 atatcgtcat cattgatgtc gatatccccg gagttaacgg tatccaggtg ttagaaacgc
     3121 tgaggaagcg ccaatatagc ggaattatta ttatcgtctc cgctaaaaat gaccattttt
     3181 acgggaaaca ttgtgctgat gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga
     3241 acaatatcat tgcagctatt gaagctgcaa aaaatggcta ctgctatttc cccttctctc
     3301 tcaaccggtt tgttggaagt ttaacgtccg accagcaaaa actcgactcc ttatcgaaac
     3361 aagaaattag tgtcatgcgg tatattcttg atggcaagga taataatgac attgctgaaa
     3421 aaatgttcat cagcaacaaa actgtcagca cttataaaag tcgcttgatg gaaaaattag
     3481 aatgcaaatc actgatggat ctttacacat tcgcacaacg taacaaaatc ggctaaccac
     3541 atgaagtttt taccctatat ttttcttctc tgttgtggtc tttggtcgac cataagtttc
     3601 gcagacggag attacatcga atatcgtggc atcagtagta acaaccgtgt cacacttgat
     3661 ccactacgtc tgagcaacaa ggaattacgt tggctcgcga gcaaaaaaaa tcttgtgatt
     3721 gcagtacaca agtcccaaac agctacgttg ttgcataccg attcgcagca acggattcgt
     3781 ggtattaatg ctgattattt aaatctttta aaaagagcgt taaatatcaa attaacactc
     3841 cgggaatacg cagatcatca aaaagcaatg gacgcgctgg aagacggtga agttgatata
     3901 gtgttatcac atttagttgc ttcgccgcct cttaatgatg acattgctgc aaccaatcct
     3961 ctgataatta cctttccggc gctggtcact acccttcacg attcaatgcg accgcttacc
     4021 tcatcaaaac cagtaaatat tgctcgagta gcaaattatc ccccagacga ggtaattcat
     4081 caatcatttc caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc
     4141 gtctcagccg gacagaatga ttactttatt ggtagtaaca tcattaccag cagta
//
LOCUS       DAZUCC010000302         4169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZUCC010000302 DAZUCC010000000
VERSION     DAZUCC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.304"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(501..944)
                     /locus_tag="ACHY09_004769"
     CDS             complement(501..944)
                     /locus_tag="ACHY09_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887618.1"
                     /translation="MQIREQGRKIQCIRTVYDKAIGRGRQTVIATLARYTTEMPTTGL
                     DELTEAERETLAEWLAKRREASQKSQEAYTAMSADRWLVTLAKAIREGQELRPEQAAA
                     IWHGMGEVGKALRKAGHAKPKAVRKGKPVDPADPKDQGEGAPKGK"
     gene            1379..1705
                     /locus_tag="ACHY09_004770"
     CDS             1379..1705
                     /locus_tag="ACHY09_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020309011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3887619.1"
                     /translation="MQQIRHYLDPNGRDHFAEWRDQVRDTKARIAIDRRIMRLELGNF
                     GDHKPLREGVWELRIDVGPGYRVYYAKAGLTVVLLLCGGDKRKQNADIDRACAYWRDW
                     QQRTGD"
     gene            1709..2020
                     /locus_tag="ACHY09_004771"
     CDS             1709..2020
                     /locus_tag="ACHY09_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3887620.1"
                     /translation="MKDRAHDTAMAEVFRNDPAYAVELLNSILEDGEQGELLIALRQM
                     AEAFGGVRQVAKSAELNPNQLYRTLSQTGNPEVRSLTAILRAMGLRLAIQPLHPTPAA
                     H"
     gene            complement(2117..2407)
                     /locus_tag="ACHY09_004772"
     CDS             complement(2117..2407)
                     /locus_tag="ACHY09_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887621.1"
                     /translation="MNEQRAIELLQAHVRDYARQRAKDVARGAETPRLAALLVQKYGK
                     GVVDALAVVFDSARSADPVMAVVDEEVSRIDPLWQEHNRERWAGRPADVVAN"
     gene            complement(2412..2780)
                     /locus_tag="ACHY09_004773"
     CDS             complement(2412..2780)
                     /locus_tag="ACHY09_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887622.1"
                     /translation="MGVDIGQLDPWVLGTYCTVLFAVFAWIAWGAFRDDELPIFPGLL
                     AVMLMVGGYAAVYEPQPDEWETFKAAHNCKVVAKRDGHSNNGIGVTTRGSVGYIIGDD
                     TPDQVAYQCDDGVTYWKNAR"
     gene            complement(2826..3281)
                     /locus_tag="ACHY09_004774"
     CDS             complement(2826..3281)
                     /locus_tag="ACHY09_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HFS3887623.1"
                     /translation="MIDFDEIRKQVAIKHNVLIGKDDPILVTVTVSDMVLGRYLELVS
                     DQYDEANRALTVSLQQQVEQSKETAGKVITDAANYVSEQVRQAVTAALADAGNDVRRQ
                     IANAQAASRDAVASGRDAQAAKTGAYLAAALAGVAALVAVAALVVVLLK"
     gene            complement(3293..4048)
                     /locus_tag="ACHY09_004775"
     CDS             complement(3293..4048)
                     /locus_tag="ACHY09_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HFS3887624.1"
                     /translation="MNDVTTPALNTLARQVHFTLQGKGGVGKSFISSLLVQYLGGKGL
                     PVTAVDTDPVNATLAGYKAFNTQRLELMENGSLIERNFDRLIEQVVEEDTNFVIDNGA
                     ASFIPLSYYIAENDAINLIGENGKQVIIHTVITGGQALRDTLGGFASLAEQMPGNAEL
                     VVWLNEFFGDIEAEGKTFEEMKVYQNNKDRVRGIVRIARQTGSTFGEDVKLMLDSKLT
                     FDEIKQSPDFGLMSKSRLSKVKTAIFEQLATII"
BASE COUNT          851 a         1255 c         1160 g          903 t
ORIGIN      
        1 taatagacgg agagttgccc ggagagccgc gccgggcctc acttttatag tctgtggtgt
       61 gtaatagacg gaggcaaaac ccgaaatgtg gataagtctg tggatggccc gtctattacc
      121 cacccggttc cgtccattac ccaccgcgcc ccgtctttta cccaccggat tccgtctatt
      181 acccactcat ccacagggct aagtccttgt cctgtaaggg aaaaatgacg atttccagac
      241 cctaaaactt cttttaaaac attaaatcta aaaaaaaacg ctctatcgag cggaaaagca
      301 ctatcaaatt gatctaactg aaaagaaaaa agcggaacca atcgcataga atcgcgtaca
      361 gtcgcattaa atcgcttaga ttcgcgcaga atcgcactac agcgcccggt ctggacgcat
      421 gtagcggctg gaagacaggt ttagacacca aaaggaaggg cgaacgagcc gtagcgccta
      481 gctcgatcac accgggccga tcatttcccc tttggtgccc cctccccttg atccttggga
      541 tcagccggat cgaccggctt gccctttctg acagccttgg gcttcgcgtg gccagccttg
      601 cgcaaggcct tgccgacttc ccccatgccg tgccagatcg cggccgcctg ttcggggcgc
      661 agctcctgcc cctccctgat ggccttggcc agcgtgacca gccaccgatc cgcagacatg
      721 gccgtgtagg cctcctgcga cttctgcgag gcttcccggc gcttggccag ccattcggcc
      781 agcgtctcgc gttcggcctc tgtcagctca tccagcccgg tcgtgggcat ttccgtcgta
      841 tagcgggcca gtgtggcaat gaccgtctgc cgaccccggc caatggcctt gtcgtacacg
      901 gtgcgaatgc actgaatttt gcggccctgt tcacgtatct gcatggtagc ccctttccgt
      961 atggtgcgga taataaacta tccgccgtaa tgatagcata ctatccgcag gcggataaaa
     1021 aacaagcatc acatttcaga tagaaatcta tccgcataca gctatcatag cagcatcata
     1081 tgccgatagg ctcactatct gcacatgcag acgctaaatt atccgcatac cgatgaaaat
     1141 ctatcacgcc atatcacgat agaaatctat cggcatacgg atgttttact atcctttgtg
     1201 cggataagta tttatcggtg tgcatgataa ggaagtatcc gcataggctt gcgcccgacc
     1261 gcaggcggaa gatgcaccaa agcagataat cacccggtca acatgcggat aaaaaattat
     1321 ccgaaattgt gacggttgcc tcttgacaag ttatcgttta taaacgacaa tatcagacat
     1381 gcagcagata cggcactacc tcgaccccaa cggacgcgac cacttcgccg agtggcgcga
     1441 tcaggtgcgc gataccaaag cgcgaatcgc catcgaccgg cgaatcatgc gcttggaact
     1501 tggcaacttc ggcgatcaca agcccctgcg tgaaggcgtg tgggagctgc gcatcgacgt
     1561 agggccgggc taccgcgttt attacgccaa ggctggcttg accgtcgtgc tgctgctctg
     1621 cggcggcgac aagcgcaagc agaacgccga cattgaccgg gcctgcgcct attggcgtga
     1681 ctggcagcaa cggacaggag attgaacgat gaaagatcga gcacacgaca cggctatggc
     1741 cgaagtgttc cgcaacgacc ccgcctacgc ggtcgagctg ctgaacagca ttcttgagga
     1801 tggcgagcaa ggcgaactct tgattgccct gcgccagatg gccgaggcct tcggaggcgt
     1861 gcggcaggtg gccaagtcgg ccgagctgaa ccctaaccag ctttaccgca cgctgtcaca
     1921 gacgggcaac ccggaggtgc gcagtctgac cgcgatcctt cgcgctatgg gcctgcgatt
     1981 ggccatccag ccgctgcacc ctaccccggc cgcccactga tagcctgcgg gctatacgcc
     2041 cgcccttgtc tttcctttgg tgcaaaacag aagcccgccg attggcgggc ttgtctgtat
     2101 gggtgctgcc ggtggtttag ttggcaacca catcagccgg gcgaccggcc cagcgttccc
     2161 ggttgtgttc ctgccagagc ggatcaatcc ggcttacttc ctcgtccacc acggccatga
     2221 ccggatcggc cgaccgggca ctatcaaaga ccacggccag agcatccact acacccttgc
     2281 cgtacttctg cacaagcaag gccgccagac gtggcgtttc tgcgcctcgt gccacgtctt
     2341 tggcccgttg tctggcgtag tccctgacgt gcgcttgcag cagctcgatg gctctttgtt
     2401 cgttcattgt gctacctcgc atttttccag taggtcacgc cgtcgtcgca ctggtaggcg
     2461 acctgatcgg gcgtatcgtc gccgatgatg tagcccacgc tgccgcgagt ggtgacgccg
     2521 attccgttgt tgctatgacc gtcacgcttc gccacgacct tgcagttgtg tgcggccttg
     2581 aacgtttccc actcgtcggg ctggggttcg tagactgcgg catagccgcc gaccatcagc
     2641 attaccgcta aaaggccggg aaaaattggc agctcgtcat cgcggaaagc accccatgcg
     2701 atccacgcaa aaacagcaaa caggactgta cagtaggtgc caagcaccca cgggtctaat
     2761 tgcccaatgt ctacgcccat gctcctttcc tcgataggga ggcaccgggc cgtagcccgg
     2821 cggcctcatt tcagtagaac gaccaccaag gccgccacgg ctaccagagc ggcaaccccg
     2881 gccaaggccg cagccaggta tgcgccggtc ttcgccgcct gcgcatcccg gccactggcc
     2941 accgcatcgc ggcttgcggc ctgcgcgttg gctatctgcc gccgcacgtc gttgccagca
     3001 tcggcgaggg cggcagtcac ggcctgccgc acctgttcgc tgacgtagtt ggccgcgtcg
     3061 gtgattacct tcccggccgt ttccttggac tgttcgacct gctgttgcag gctcacggtc
     3121 aaggccctgt tggcttcgtc gtactggtcg gaaaccagtt cgaggtagcg gcccaagacc
     3181 atatcgctga ccgtgaccgt gaccagtatc ggatcgtcct tgccgatcag cacgttgtgc
     3241 ttgatcgcca cctgtttccg tatctcgtca aagtcgatca ttggcgaccc cctcagatga
     3301 tggtggccaa ctgctcgaag atcgccgttt tgaccttcga gagacgagac ttggacatga
     3361 ggccgaagtc tggcgactgc ttgatttcat cgaaggtcag cttgctatcg agcatcagtt
     3421 tcacgtcctc gccgaacgtg ctgccggtct ggcgagcgat acgcacgatg ccgcgcacgc
     3481 gatccttgtt gttctggtag accttcattt cctcgaaggt tttgccttcg gcctcaatgt
     3541 cgccgaagaa ctcattgagc cagacgacca gctcggcatt gcccggcatc tgctcggcca
     3601 gagaggcgaa gccgccgagg gtgtcgcgca gggcttggcc gccggtgatg accgtgtgaa
     3661 tgatgacctg cttgccgttc tcgccaatca ggttgatggc gtcgttttcg gcgatgtagt
     3721 aggacagcgg gatgaacgac gccgcgccgt tgtcgatcac gaaattggtg tcttcctcga
     3781 ctacctgctc aatgaggcgg tcgaaattac gttcgatcag cgagccgttt tccatcagct
     3841 caaggcgctg ggtgttaaac gccttgtaac cggcgagggt ggcattgacc ggatcagtgt
     3901 ccacggccgt gaccggtagg cccttgccgc cgaggtactg gacgagcagg gacgagataa
     3961 aggacttgcc gacgccgccc ttgccttgca gcgtgaaatg aacctgacgg gcgagggtgt
     4021 tcagggccgg agtggttacg tcgttcatag gtgcggtgct cctttcagat gaggtcgtcg
     4081 cggtcgttgc cgctgtttgg gggttcgcgc ccatttcctc taaaagtctc accttgctta
     4141 ggtaattaat tgaatttatt tataaaacg
//
LOCUS       DAZUCC010000303         4137 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZUCC010000303 DAZUCC010000000
VERSION     DAZUCC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.305"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            227..835
                     /locus_tag="ACHY09_004776"
     CDS             227..835
                     /locus_tag="ACHY09_004776"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFS3887625.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="ACHY09_004777"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="ACHY09_004777"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFS3887626.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..>4137
                     /gene="selB"
                     /locus_tag="ACHY09_004778"
     CDS             2321..>4137
                     /gene="selB"
                     /locus_tag="ACHY09_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFS3887627.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
BASE COUNT          932 a         1043 c         1220 g          942 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgctgacagg cgaaacccta cagatttata
       61 agaaagcctt aagtgagata tctgtcagat aagaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat tgggaagtaa caaaccatca acattttctg ccctttttcc
      181 tatcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      301 tttcgaattt attaatgaac tgccctataa cgcggataac ggcgtggcgc aatttaaccc
      361 gttagggaaa gtgccggtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat
      421 catcgctgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccgct
      481 ggagtcgttg cgggtgcgca aaattgaggc actggcggat ggcattatgg atgccgggct
      541 ggtatcggtg cgtgaacagg cgcgtccagc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgcgag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 gctcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggctggtg tgtcgaccgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgc tgcgtcagat gcttgacgaa gcgcgagaag
     1081 tgattcgtga tagccagacg ctgcccgcat ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaggcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1201 tgctgcatac caatcttggg cgagctttac aggcagaagc cgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc
     1321 gcgatcgggc gctggcgcag ctgctgtgcc gcattacggg agcggaagat gcctgtatcg
     1381 ttaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg
     1441 tggtggtatc tcgcggcgaa ctggtggaga ttggcggcgc gtttcgtatt cctgatgtca
     1501 tgcgtcaggc aggctgcacc ctacacgaag tggggaccac caaccgcacg cacgctaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cgctgttgat gaaagtacat accagtaact
     1621 acagcattca gggattcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     1681 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggttgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gtttctccgg tgacaagttg ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc tccccttgcc gcacattacg gcgcggagtt tgcagtccag gttatgccat
     2101 gtctttcgca gattggcagt ggttcactgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgccgatcg
     2401 tctgccggaa gaaaaaaagc gcggcatgac tatcgatctc ggctatgcct actggccgca
     2461 gccggatggc cgcgtgcctg gttttatcga cgttcccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgtcggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 tgtgatggca cagacccgtg agcatctggc gattttgcag ctgactggta acccgatgct
     2641 gacagtggcg ctgaccaaag ccgatcgcgt ggacgaagcg cgtgttgatg aggttgaacg
     2701 ccaggtaaag gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt taccagaacg
     2821 cgaacacgcc agccaacata gtttccgcct cgcgatcgac cgtgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 ctggctgact ggtgtaaata aaccgatgcg cgtgcgtgcg ctgcatgcgc aaaatcagcc
     3001 aacagaaact gcccatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgcg ccgccggagc cgttcacacg
     3121 ggtgattgtc gagcttcaaa cccatacacc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtca cgggacgcgt ttcgctgctg gaagataacc ttgccgagct
     3241 ggtgttcgac acgccgctgt ggctggcgga taacgatcgc ttagtgttgc gcgatatctc
     3301 ggcccgcaac acgctggcgg gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa
     3361 gcgtaagccg gaatatctgc aatggctggc gtctcttgca cgggcgcaga gcgatgccga
     3421 tgcgttatct gttcatctgg aacgcggagc ggttaacctt gcggatttcg cctgggcacg
     3481 gcagctcaac ggcgaaggga tgcgcgaact gctgcaacag cctggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc tcgctggcag cggaaaattc tcgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctggtcct gggcgagaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct ggtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atcctcagtc atcacggctg gctgcatctg ccggatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagcca ctgtttggtg acgaaccgtg
     3841 gtgggtgcga gacctggcaa aagaaacggg aaccgacgag caggcaatgc gcctgacgct
     3901 acgccaggcg gcgcaacagg ggataattac tgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggaat gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcaaatcct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcg
//
LOCUS       DAZUCC010000304         4093 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZUCC010000304 DAZUCC010000000
VERSION     DAZUCC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.306"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            500..1066
                     /locus_tag="ACHY09_004779"
     CDS             500..1066
                     /locus_tag="ACHY09_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS3887628.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMLARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWIDRQRVKKIY
                     PFAERIAECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRNLQK"
     gene            1324..1899
                     /locus_tag="ACHY09_004780"
     CDS             1324..1899
                     /locus_tag="ACHY09_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887629.1"
                     /translation="MNAIPYFDYSLAPFWPSYQSKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVLLDSCSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGLF"
     gene            1968..2204
                     /locus_tag="ACHY09_004781"
     CDS             1968..2204
                     /locus_tag="ACHY09_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3887630.1"
                     /translation="MLTSITGHDSILLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            2486..2998
                     /locus_tag="ACHY09_004782"
     CDS             2486..2998
                     /locus_tag="ACHY09_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887631.1"
                     /translation="MQITKALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHRGATIRCLLLLSQTSICH
                     PRASVTVDEECSQLVDLLQQTWKVISAGGQCRVEKRFQVVRGDTSGQYVALKTVVQSF
                     VLPVMTTINW"
     gene            3087..3710
                     /locus_tag="ACHY09_004783"
     CDS             3087..3710
                     /locus_tag="ACHY09_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3887632.1"
                     /translation="MRTRIDYLANKYSFTELNESPRLRWQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDISQKNVMFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNESTSSPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRQSGMPVVTTSVEVSDNLTGTTRKIPAYHL"
     gene            3806..4039
                     /locus_tag="ACHY09_004784"
     CDS             3806..4039
                     /locus_tag="ACHY09_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887633.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
BASE COUNT          987 a          965 c         1019 g         1122 t
ORIGIN      
        1 ttctggtacc gtgtacccgt taacgttcat atcttgaaca tgagttgttt ttaaccgatt
       61 aaatgagtgc gtgtaagttt attgggttac aggtatatgt tggccttgta cacgtttttt
      121 cagactcact gagagcaagt ttgtagttta tgatgttatt ggtcgtaata tgttttttga
      181 actattggtc gtaatatgtt ttttgaacta ttggtcgtgg gtttttatcg cgtgttttgc
      241 agtatttcgt gatgtatgtt tattgattct gtattttatt cgctcctcgt ttatctcatc
      301 tctttagcta ataccatcag ataatccatt tctttctgca taatgctgcg tatcgttaat
      361 aacccgtcgt atccattctg ctacagcatg cctgataaat accatctgta agttattacc
      421 gttttagatc tgattatgag cgaaagcatt aattcgttca cagagcttaa aacatcatta
      481 actttcagga gtcatcaaca tgcctaaatc ttacacacca aactggtttt ttaccgcttt
      541 acttgacaat cacatcaatc aaatgctggc acgttattcc tgcctgcgag ctttacggat
      601 ggatttcttc tacaggaaag atacgcctga tttcttacaa cctgatcatc gctggcttga
      661 attgcagttg cgtatgctgc tggagcaggt ggaacaattt gaaaatatcg ttggcttctt
      721 ctgggtgatt gaatggacgg ctgatcatgg ttttcatgca catgcggttt tctggatcga
      781 tcgtcagagg gttaaaaaaa tatatccctt tgcggagcgg atagctgaat gctggcggtc
      841 tattacgcat aacagcggtt cggcacaccg ctgtacatat cagccgcatt atacatacaa
      901 catcaacatt cctgtgcgcc acaacgatcc tgaaagcatc gataatattc gcggtgccct
      961 gcattatctg gcgaaagaag aacaaaaaga cgggctgtgt gcctacggct gcagtgaagt
     1021 tcctgagcgt cctgccgcag ggcgtccacg taatcttcag aagtgaagct taaaccttga
     1081 gtttcctggc ccggaaacac tccgttagta aaaacttacc gccctgctta atgatgtgaa
     1141 ccgaagtcaa tggagatcat tcaccctgaa ccacatccgg tgttttgttc cttgtcagtt
     1201 acctgatctt cccgttttgc cccggttttt cacttattcc atcaacccca tccgcaaacc
     1261 ataacacgtc agcttattca cgggcaggac gcattgtggg ctgcgcataa tggagcatat
     1321 cttatgaatg ctatccctta tttcgactat agcctggcac ccttctggcc atcttatcag
     1381 agcaaagtca tcggcgttct tgagcgtgcg ctgcgtgagc agtccggctc acggatacgg
     1441 cggatcctgc ttcgtctgcc gtgtgaatat gacaatacct tcaacagcag aacgacctgg
     1501 tttggtatgg attttatcga aaccgtcagt gcgctgatga atgcgaaacc cggacgcgac
     1561 ctttgctggc tcctgacccg tcatcctgaa aagccggaat accacgtggt gctgtgtgtc
     1621 agacaggagt atttcgacgg ccctgaactg gatcggctaa ttctggatgc ctggagcaac
     1681 gtactgggct tcgcgtcacc gggtgaagca gcaccgtacc agaagcagat caccagggat
     1741 gtactgctgg acagttgttc accggactgc gaagacatcc tcaaagactt gatctgggcg
     1801 ttcagtgatt tcgcccgcga tcgtcgtggt gtgtgcgatc cggaagcccg ttgcctcgcc
     1861 ggtaatcccg gctatcccgg atctgcagga ctgttctgaa agctcagttc agcagattca
     1921 ccagcatttg cccaacctcc ataaatctca cagaggacat cctgactatg ttgacctcaa
     1981 taacaggcca cgacagtata ttgctgcgtg ccgacgatcc cctgatcgac atgaactaca
     2041 tcaccagttt caccggtatg accgataaat ggttttacaa gctgatcagt gaaggtcatt
     2101 ttcctaaacc cataaaactg ggtcgcagca gccgttggta caaaagtgaa gtggagcagt
     2161 ggatgcagca gcgaattgag gaatcacgag gagcagctgc atgaaacgtg ttgtgatgcc
     2221 ggtacgctgg caatgtgcaa aatgtcggcg ctggtattgt ggagatcagc cctgctcctg
     2281 gtgctggcgc tgacaactcc cgctcatctt tctgctgact cctctggtca gccaactgtt
     2341 tgtcatcatt tcctgactga ttcgtcattc ccttcgtccc gccagtcact ggtgtttgta
     2401 tcgtatcgtg ccggtgttga tgtgcgcttt ccgtgtgtct gcaccggctt aacaaaattc
     2461 aacagggttt gaaaaggaac atttcgtgca aataaccaaa gccttaattt cagagccggg
     2521 agacatccgg cgttttgttc agcaggcggt ggaccactgg ccaaatctgt tggcatttca
     2581 cttcacgctc tattcggcag aaggaaacat caacgggcaa cagattcatg cattctgtac
     2641 ttccttttac cgacaggtgc acgaacgtat tactgagcgt aatcacacgg ccagtccatc
     2701 ctcgccggtg gtattacgct ggttgcggga acaacataga ggagcaacaa ttcgatgcct
     2761 gttgctgctc agccagacga gtatttgtca cccgcgagcc agtgtcacag ttgatgaaga
     2821 atgttcacaa ctggtggatt tactacaaca gacctggaag gtgataagcg ctggcggaca
     2881 atgccgggtg gaaaagcgtt ttcaggtcgt ccggggtgat acatctggcc agtatgttgc
     2941 gttaaaaaca gtcgttcaat cctttgtgtt accggttatg acaacaatca actggtgatg
     3001 gtcgtgtgaa ccagctgcgc ttccgttgat tcttatggac tgataaagtt ttgtcagatg
     3061 aatttttata cagaattctt ttcagtatgc gtacacgaat tgattatctg gcgaataaat
     3121 acagttttac agagctgaat gaatcccccc gtcttcgctg gcagtggcag gatgttctgg
     3181 aggagtgccg gcagacagag gccgggccag aagaacggct gcgtattgct ctgctgaatg
     3241 tggattacgt caccagtttt gaactgcctt ttcgcttgtt gcttatccgt acaccacaac
     3301 tgattgccgc gctgcgtgaa gaatgggaca tcagccagaa aaatgtgatg ttcaacgata
     3361 aacgctttgg ctgcgtgtac agcctgaagg ccagcctttc cggtgtgccg gatacatacc
     3421 ggtatcatct gtctcaccgt attcgtcgcg tggttggaaa tgaaagcaca tcatcgccat
     3481 atcagcaggt tgccagggaa gtgaaagcgc cccgtgaacg gctgaagtat gcgctggagg
     3541 cgggcttact ggtgaccgca ctggacggcc ttttctggtc tggcagtcag cgtattgcgg
     3601 ctgatgtcct gagactaaga cagagcggaa tgccggtggt gacaacgtcc gtggaagtga
     3661 gcgataacct gacgggaaca acccgcaaaa taccggcata ccatctctga tacgaccatc
     3721 agggggaata ttgctctggc agcagcagta cgacattctt tatactttat ccccgtgcct
     3781 gaaaacatgt gctaaggaca cgggaatagc aaggtccgat tacgatatta tcaatctgtc
     3841 tccggaacat gaactgaatg agtggctggc agagagaggt tatgccggac tggcggataa
     3901 ccggaaccgc ctggcagagg tggttgcctg caaattgcag gacagcttct gtatgaacgt
     3961 ttcctgggat gctctgaacg cagcatacag tgaacatccc gagtggtttt cagggcttgt
     4021 ctccggggat gagaattaaa aagaggatta tgctgccatc aggcagtgtg attgccggca
     4081 gggatttttt ctt
//
LOCUS       DAZUCC010000305         4067 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZUCC010000305 DAZUCC010000000
VERSION     DAZUCC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.307"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(18..803)
                     /gene="ygcW"
                     /locus_tag="ACHY09_004785"
     CDS             complement(18..803)
                     /gene="ygcW"
                     /locus_tag="ACHY09_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3887634.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGKTKEMIEKQGVEMDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            1122..2399
                     /gene="yqcE"
                     /locus_tag="ACHY09_004786"
     CDS             1122..2399
                     /gene="yqcE"
                     /locus_tag="ACHY09_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFS3887635.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSTNNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            2426..3904
                     /gene="ygcE"
                     /locus_tag="ACHY09_004787"
     CDS             2426..3904
                     /gene="ygcE"
                     /locus_tag="ACHY09_004787"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFS3887636.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWTSLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYDHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTILGHITPQAALATHFPAGLSVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMIRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         1052 a          926 c          991 g         1098 t
ORIGIN      
        1 gaaaccaccg ttatggatta gcgcactaaa taaccgccat cgaccactaa taaatgcccg
       61 ttgacataat tcgatgcctg acttgcgaga aacacggctg cgcccattaa atcctgagta
      121 tcgccccaac ggtttgccgg aatatgatca agaacgcgct gattggtttc tggattactg
      181 cgtgtcgcca gcgtaatatc tgttgcataa tagccagggg caataccatt tacctgaata
      241 ttatattgac ctagttcatc acaataagct ttggtgaacc cggcaagagc atgtttagtg
      301 gcagaatatg caggtgacca ttgtccacct aagtaagaga acaatgaaca gatattaata
      361 attttaccgc ttttctgcgg gatcataatt tttgccgctt catagcttaa ctcgaatgcg
      421 gcggtcaggt tcacatcaat catcgggtcc cagtcggcac gaccgaaatc cagcacctta
      481 ttcagcttac aaataccggc attgttaacc agaatatcaa ctgttccgaa acgctcacag
      541 caagcagcga taatcttctg cggcgcgcct tctgcggtga tatccacctg catgaagtcc
      601 atctcaacac cctgtttttc aatcatttcc tttgttttgc cgttatcttt aacgaagctt
      661 ggaataaaga tatttgcacc agctttggcc agcgccatgg caaatgcctg gcctaaaccg
      721 ctattcccgc cggtaacaat tgcggtttta cctttcaggg agaaaaaatc cattgagaac
      781 gcattgagag attcgattga catagttagc tccaatttcc gtaaggcaaa aaaaaagaaa
      841 ggtaacttcc cccgcgagag ggaagttacc tttctcatta tctctggtaa ctggagataa
      901 tttttatcat aagggcataa tcgctaatgt gatcgtaatc acagtgttat attcgaattg
      961 tcaaataatt ttaaaaagca gaagcgtgat tactctcaca caacaccact gctttccatg
     1021 ttatttctta ttgcaattac tagagacctg agaaaagtaa tccacctgaa agggcggctt
     1081 acttttctct tttttttgtc tattacaaat gggaatacga tatgcaacac aactcatatc
     1141 gccgttggat aaccctcgcg ataattagtt ttagcggcgg cgttagtttc gatctggctt
     1201 atttacgtta tatttatcaa attcccatgg cgaaatttat gggattcagc aataccgaga
     1261 taggtttaat aatgagtacc tttggtattg cggctattat tctttatgcc cccagcggcg
     1321 ttattgccga taaattttca caccgcaaaa tgattacttc cgcgatgatc attaccggat
     1381 tactgggtct gttaatggca acatatccac cgctgtgggt aatgctctgt attcaggtcg
     1441 cctttgcgat aacgacgatt ttaatgctgt ggtcggtgtc gattaaagcc gcctcattgc
     1501 tgggcgatca tagtgagcaa gggaaaatta tgggctggat ggaagggctg cgcggcgtcg
     1561 gtgtaatgtc gctggcggtg tttaccatgt gggtcttttc tcgctttgcg ccggatgaca
     1621 gcgccagcct gaaaacagtc attattatct acagtgtggt ttacatcttg ttggggattc
     1681 tgtgctggtt ttttgttagc gataacaaca acctgcgcag taccaataac gaagaaaaac
     1741 agtcattcca gcttagcgat atcctggccg ttttgcgtat cagtaccacc tggtattgca
     1801 gcatggtgat ttttggtgtc ttcaccatct acgccattct gagttactcc accaactatc
     1861 tgaccgaaat gtacggcatg tcgctggtgg cggcgagcta catggggatt gtgatcaaca
     1921 aaatcttccg cgcgctgtgc ggcccacttg gcggcattat caccacctac agtaaagtga
     1981 aatcccctac ccgcgtgatc caaatccttt ccgtactcgg cctgctggcg ttaactgccc
     2041 tgctcgtcac gaactctaac ccgcaatcgg tcgcgatggg gattggcctg attttactgc
     2101 tgggattcac ctgttacgcc tcacgcgggc tgtactgggc ctgcccaggc gaagcgagaa
     2161 caccgtctta cattatgggc accacggtag gtatttgttc ggtgattgga ttcctgccgg
     2221 atgtcttcgt ttacccaatt atcggccact ggcaagacac cctgcccgca gcagaagcct
     2281 accgcaatat gtggctgatg ggcatggcgg cgcttggcat ggtgattgtc tttacctttt
     2341 tgctgttcca aaaaattcgt actgctgata gcgcccccgc aatggctagc agcaagtaag
     2401 ccaggaaaat tacaggagaa aaatcatgtc gaaaaaatac atcataggga ttgatggcgg
     2461 aagtcagagc acaaaagtgg tgatgtacga tctggaaggt aatgtggttt gcgaaggtaa
     2521 aggcttattg cagccgatgc atacgccaga tgccgatact gccgaacatc ctgacgacga
     2581 tttatggaca tcgttatgct ttgccggtca cgatttgatg agccagtttg ccggaaataa
     2641 agaagatatt gtcggtattg gtctgggctc catccgttgc tgtcgtgcgt tattgaaagc
     2701 cgatggcacg cccgctgcgc cattgattag ctggcaggat gcacgtgtaa cacgtcccta
     2761 cgatcatacc aatccagatg tggcctatgt cacctctttt tcgggttatc tgacgcatcg
     2821 cttaaccggc gagtttaaag ataatatcgc caactatttt ggtcagtggc cggtggatta
     2881 taagagctgg acatggagtg aagatgctgc ggtaatggat aagtttaata tcccccgtca
     2941 tatgctgttt gatgtgcaaa tgcctggcac cattcttggg catatcacac cacaagctgc
     3001 gctggcgaca catttcccgg ctgggctgtc ggttgtttgt accaccagtg ataaaccggt
     3061 agaagctctg ggagccgggt tactggatga tgaaactgcg gtaatttctc ttggcactta
     3121 catcgcattg atgatgaacg gcaaagcctt gccgaaagat ccggtggcgt actggccgat
     3181 tatgtcttct attccgcaaa cattgctgta tgaaggttac ggtattcgca aaggcatgtg
     3241 gacggtgagc tggctgcgcg acatgttagg cgagtcgtta attcaggatg ccagggcgca
     3301 ggatctttca ccggaagatt tactcaacaa gaaagcctct agtgtgccac ctggctgtaa
     3361 tgggctgatg acggtgctgg actggctgac caatccgtgg gaaccgtaca aacgcgggat
     3421 tatgatcggc tttgattcca gtatggatta cgcatggata tatcgttcga tactggaaag
     3481 cgtggcgctg acgctgaaga acaattacga caatatgtgt aatgaaatga atcactttgc
     3541 gaagcatgta atcattactg gcggcggttc gaacagcgat ctgtttatgc agatttttgc
     3601 cgacgtgttc aaccttccgg cacgacgtaa cgccattaac ggttgtgcaa gtctgggggc
     3661 agcgattaat accgcggtag gtctggggct atacccggat tacgcaacgg ctgtcgacaa
     3721 aatgattcgc gtgaaagata tctttatacc gattgagagc aatgccaaac gctacgacgc
     3781 gatgaataaa ggcattttca aagacctaac caaacatact gatgtgatcc tgaaaaaatc
     3841 gtatgaagtg atgcatgggg aattggggaa tgcggattcg atccagagct ggtcgaatgc
     3901 gtaagtgagg aaggccgggc gggaaactgc ccggcctgaa catacctgaa tggttatccc
     3961 cgctgacgcg gggaacataa gttcgacaac ttaaaaaatg ataacaagtc cggtttatcc
     4021 ccgctgatgc agggaacata ataaaaactt taactagttc ggtttat
//
LOCUS       DAZUCC010000306         4062 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZUCC010000306 DAZUCC010000000
VERSION     DAZUCC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.308"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..610
                     /locus_tag="ACHY09_004788"
     CDS             <1..610
                     /locus_tag="ACHY09_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS3887637.1"
                     /translation="IKLKKQFNFKLIEHEWENIQHIICSLSRKTTIQSTVVKKLSNTK
                     RSNKTLAALREYDRVIKCIYVLDYADNKTLRQFVQQALNRGEAYHQLRRAIASVNGNQ
                     FRGGNDYQIDQWNDCARLIANCVIYYNSAILSRLVEKFEKENNKKAIDVLANLSPVAW
                     RHILLGGNYSFEDQVAITNLDSLLEGVDPLSDIGDIESEYDG"
     gene            677..1354
                     /locus_tag="ACHY09_004789"
                     /pseudo
     CDS             677..1354
                     /locus_tag="ACHY09_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            1423..2598
                     /gene="senB"
                     /locus_tag="ACHY09_004790"
     CDS             1423..2598
                     /gene="senB"
                     /locus_tag="ACHY09_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFS3887638.1"
                     /translation="MNIFTLSKTPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSGDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(3228..3686)
                     /locus_tag="ACHY09_004791"
                     /pseudo
     CDS             complement(3228..3686)
                     /locus_tag="ACHY09_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612075.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1194 a          886 c          942 g         1040 t
ORIGIN      
        1 tataaaactt aaaaaacaat ttaactttaa gctcattgaa catgaatggg aaaacatcca
       61 acatattatt tgttctttaa gcagaaaaac aaccattcaa agtacggttg ttaagaagtt
      121 atctaataca aagcgtagca acaagacatt agcagcactt cgggagtatg accgagttat
      181 aaaatgcata tatgtcctgg attatgctga caataaaacc ttaaggcaat ttgtgcaaca
      241 agcactgaac agaggtgaag cttatcatca actaagaaga gcaatagcat cagtaaatgg
      301 aaatcaattt cgcggtggta atgactacca aatagatcaa tggaacgact gtgcgaggtt
      361 aatagctaat tgtgtaatct attataattc agcaatatta tccaggctgg tcgagaaatt
      421 tgaaaaagaa aataataaaa aagcgataga tgttctggct aacttatccc cagtagcatg
      481 gagacacatt ctccttggag ggaattattc ttttgaagat caagttgcga tcacaaattt
      541 ggacagtctt ttggagggag tagacccact aagtgatatc ggagatattg agtccgaata
      601 tgacggttaa caacatgata aataatggtt tttatgctaa gagtgagact tttggaggaa
      661 atgggcgcag aaccccttta aggctctggc gtaccgcagc aggatccaga actacatcta
      721 cagcgagtct tatctggttt gttctggagg tcgtaaatgc agtatggctg aggtgattgg
      781 caatgactgg gagggcatta gcgatgaata cagcgacaat atgtacatct acgttaactc
      841 ggcaagcgac gttatcgcaa agggcttcga actggagatg gattatgatg caggttttgc
      901 ttttggccga ctctctttca gccagcagca aacagaccag ccaacctcca tcgccagcac
      961 ctactttggc gcaggggata tgaccgaact gcccagaaaa tacatgacgc tggatactgg
     1021 tgttcgcttc ttcgataacg cgttgaccct gggcactatc ataaaataca caggcaaggc
     1081 tcgtcgcctg tcgcctgatt ttgagcagga cgaacatacc ggcgcaataa tcaaacagga
     1141 tttgccgcag atcccaacga ttatcgatct ctatggtact tacgagtaca accgcaacct
     1201 gacactgaaa ctttcggtac aaaacctgat gaacagagat tattcggagg cgctgaataa
     1261 gctcaacatg atgccaggtc ttggtgacga gacccaccca gccaattccg cgcgtggcag
     1321 aacatggata tttggcgggg acattcgttt ctgatgacaa tttccgctag gttcagacaa
     1381 tacgttttct ctcttatgtc aattttattg caggaacgaa aaatgaatat tttcacttta
     1441 tccaaaacac cgctatacct gttaatttca ctatttttac ccacgatggc catggctatc
     1501 gatccacctg aacgcgaact ttcgcgattt gccctgaaaa cgaattacct tcagtcccct
     1561 gatgaaggcg tctatgaact ggcgtttgat aatgccagta aaaaggtgtt tgcagcagtc
     1621 accgatcgtg taaatcgtga agccaataaa ggctatctgt attcgtttaa ttcagattcg
     1681 ctgaaagtcg aaaataaata cacgatgcca taccgggcat tttcgctggc gataaatcag
     1741 gataaacatc agctctatat cggacacacc cagtcagcgt ccctgcgtat cagtatgttt
     1801 gacaccccaa ccggcaaact ggtaagaacc agcgacaggt taagttttaa agcggcaaac
     1861 gctgcagatt cgcgttttga gcatttgcgc catatggttt acagccagga ttccgatacc
     1921 ctgtttgtga gttatagcaa tatgctgaaa acggccgagg gcatgaagcc tctgcataag
     1981 ctgttaatgc tcgacgggac gacgcttgcc ttaaaaggcg aggttaagga tgcttacaaa
     2041 ggtacagcgt atggtctgac gatggatgaa aaaacacaga aaatctacgt tggcggaaga
     2101 gattacatca acgaaattga tgcgaaaaat cagacgctgc tgcgtaccat cccgttgaaa
     2161 gatccgagac cacaaatcac aagtgtgcag aatctggcgg tggactccgc ttctgaccgt
     2221 gcctttgtgg tggtattcga ccatgacgat cgttccggta caaaagatgg actctatatt
     2281 tttgacttac gcgacggtaa acagcttggc tatgtgcaca caggagccgg agctaacgcg
     2341 gtgaaataca atccgaaata taacgaactg tatgtcacca acttcactag cggcaccatc
     2401 agcgtagtgg atgccaccaa atacagcatc acccgtgaat ttaacatgcc ggtctaccca
     2461 aaccagatgg tgttgtcggg tgatatggat accctttaca ttggcatcaa agaaggcttt
     2521 aaccgcgatt gggatcctga tgtgtttgtg gaaggagcta aagaacgtat tctgagcatt
     2581 gatttgaaaa agtcgtgatc gggggaaaac atgggataaa aactccgtta ttccactaaa
     2641 ctggccggac taggaaaagc aaacatctct gtcctgccgg tacgcctgtt acgccgctcc
     2701 atgaggctga ggaatctcca gaaaacgaga caggcacagc attgtacgat caactgattt
     2761 gtgccaataa ttcaatgaaa tcacagctat gcctgttcat ggggttctag gccggaaccc
     2821 cagatttttc cgattgtttg ttttctaact gattattcag tagttttaga gggctgacgc
     2881 tcagtggaac gaaaactcac gttaagaggt tttgactatg ataaaagtca aagtaggggt
     2941 gagtttggat atggaaatta ttgtactata agggcttccc cgaacagtca acgtcggtat
     3001 ttatggtttg tctgcactac agttaaaaat aaaggggaga gactgcggta ctataaacgg
     3061 tcataataaa tcgttatcga ttcggaccct gatgaaagac gcgcgtgagg ctcttgttcg
     3121 ttaagcggat acgtatatcc tgttcaataa tcagatatac ctcacggggt ctaacccttt
     3181 atcatcaacg agttgcaggt cagatagtca tacggttgct gcgtgcttta gctggctgaa
     3241 tacagtctga aatcatcacc gtaatgcaat gatgcccagc acagccttgc atttttggcg
     3301 gcgatggcca caacagcgcg ccagtatcct ctgcgttcga ccagtgaaca gacccaacga
     3361 ctgaatgagt cagtcctttt ctcagcgcca atcagtactg aacgagcacc ctgaaccaga
     3421 agcgttcgca gatacgaatc accggctttt gttatcctgc ctagcttcga ctttccgccg
     3481 ctgctgtatt gtgatggagt cagtcccagc caggccgcca gttgccgtcc atttttaaaa
     3541 tcatgtgcat taccgataca ggcaaccagc gcacaggcag ttgtgggacc aattcctttt
     3601 agctccatca gccgctgact gcggtgatct gttctggcca tacgggacaa aattttgtca
     3661 tattcagtga tgttagcttc aatgcggggt cagtttggag atcgtaaaat tttgttcgtt
     3721 aagcacatta tttaaccaat agatatgcgt aacggattga ttttgcgtca acggctatcg
     3781 cttttaagtt atcgccattg aggtattgca tttaggttat ccgatagagg tcaaatcctc
     3841 tgaaaacgcc gtatagcacg ggagtacacc ctaccgcaat agcgcaaccg tacaatattg
     3901 tacgtttccc aaacgcagcc taggaagcac gtcgtcagta ttggagaaaa cttacaaacg
     3961 ctcgccgcag ccccagcgac cgaacgttag tgagcgagtg ggcgaggaag cggaaaagtg
     4021 tcaggtctga attgagcaca tacgccagaa attatatagc aa
//
LOCUS       DAZUCC010000307         4059 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZUCC010000307 DAZUCC010000000
VERSION     DAZUCC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.309"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..1700
                     /gene="dpiB"
                     /locus_tag="ACHY09_004792"
     CDS             42..1700
                     /gene="dpiB"
                     /locus_tag="ACHY09_004792"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFS3887639.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIHSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1669..2349
                     /gene="dpiA"
                     /locus_tag="ACHY09_004793"
                     /gene_synonym="citB"
     CDS             1669..2349
                     /gene="dpiA"
                     /locus_tag="ACHY09_004793"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFS3887640.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2390..3775)
                     /gene="dcuC"
                     /locus_tag="ACHY09_004794"
     CDS             complement(2390..3775)
                     /gene="dcuC"
                     /locus_tag="ACHY09_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFS3887641.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1110 a          954 c         1047 g          948 t
ORIGIN      
        1 acattcgtca aagaggctcg ggacaacccg caaggaaaac aatgttgcag cttaacgaga
       61 ataaacagtt tgcatttttc caaagactgg catttccgct gcgtatcttt ttgctgattc
      121 tggtgttctc aatatttgtc attgcagccc tggcgcaata ttttacggcc agttttgagg
      181 actatttaac gcttcatgtg cgcgatatgg caatgaatca ggcaaaaatt attgcttcta
      241 atgacagtat catcagcgca gtgaaaacgc gtgactacaa gaggctggcg accatcgctg
      301 ataaattaca aagagatact gatttcgatt atgtggtgat tggcgatcgg cactcgattc
      361 gtctttacca tcctaatccg gagaaaattg gttatcctat gcagttcacc aaaccgggcg
      421 cgctggagaa aggggagagc tacttcatca ccgggaaagg gtcaattggc atggcgatgc
      481 gtgccaaaac gccaatcttt gatgacgatg gaaaagtcat cggcgtggtg tcgattggct
      541 acctggtgag taaaatcgat agctggcggg ctgagttttt attaccgatg gctggcgtgt
      601 ttgtcgtgct gttagggatt ctgatgttgc tgtcgtggtt cctggccgcg catatccgtc
      661 ggcagatgat gggcatggag ccaaagcaaa tcgcacgcgt ggtccgtcag caagaggcgc
      721 tgtttagttc ggtttatgaa gggctcattg cggtggatcc gcacggttac attaccgcca
      781 tcaatcgtaa cgcaagaaag atgctggggc tgagctcccc cggacggcaa tggttgggta
      841 aacccattgc tgaagtggtc aggcccgccg atttctttac cgaacagatt gatgaaaaac
      901 gtcaggatgt ggtggcgaac tttaacggtc tgagcgttat tgccaaccgg gaagctattc
      961 attctggtga tgatttgctg ggggccatta tcagctttcg tagtaaagac gaaatatcca
     1021 ccctcaatgc acaactgacg caaattaaac aatacgtcga gagcctgcgc acattgcgac
     1081 acgagcatct caattggatg tcgacgctca atggtctgtt gcagatgaaa gagtatgatc
     1141 gcgtgttggc gatggtgcag ggggagtctc aggcccagca acagcttatt gatagcctgc
     1201 gcgaggcgtt tgccgatcgc caggtggcgg ggctgctttt tggtaaagtg cagcgcgccc
     1261 gcgaactggg gctaaaaatg gtcatcgtcc ccggaagtca gctttcgcaa ctgccgccag
     1321 gactggacag caccgagttt gcagccattg ttggcaattt acttgataac gccttcgaag
     1381 ccagcctgcg tagcgatgaa ggaaacaaga tcgttgaatt attcctcagc gatgaaggcg
     1441 atgatgtggt gattgaagtc gccgatcagg gctgcggcgt tccagagtct ctacgagaca
     1501 aaatatttga gcagggtgtc agtacgcgtg ctgacgagcc cggcgaacat ggcattgggt
     1561 tgtacttgat tgccagctac gtaacgcgct gcggtggtgt tatcactctc gaagataatg
     1621 atccctgcgg taccttattt tcaatctata ttccgaaagt gaaacctaat gacagctcca
     1681 ttaaccctat tgatcgttga ggacgaaacg ccgctggcag agatgcatgc ggaatatatt
     1741 cgtcacattc ccggattcag tcagatatta ctggcgggaa acctggcgca ggcccgaatg
     1801 atgatcgagc gttttaagcc ggggctaatc ttgctcgata actatcttcc tgacggtaga
     1861 gggattaatt tactgcatga actggtgcag gcgcattatc ccggcgacgt ggtgtttacc
     1921 actgcagcca gtgatatgga aacggtgtct gaagccgtac gttgtggtgt atttgattat
     1981 ctcattaagc caattgccta tgaacggctg gggcaaacgc taacccgttt ccgccagcgt
     2041 aaacatatgc tggaaagtat tgatagcgcc agccagaagc aaatcgatga gatgtttaat
     2101 gcctatgctc gcggtgaacc caaagacgaa ctgccgaccg gcattgaccc cttgacgcta
     2161 aacgcggtgc gaaaactgtt taaagagcct ggtgtgcaac atacggcaga aacggtggcg
     2221 caggcactga ctatcagccg caccactgcc agacgttatc ttgaatattg cgccagccgc
     2281 catctgatta ttgctgaaat tgttcacggc aaagttggca gaccacaacg catataccac
     2341 agtgggtgac atgataaagc cggaggaaac ttgcctccgg catgactatt tacttgcctg
     2401 tgaccgcggc tgccgttcct ggcaccatca gctctgtagc aacgataaca atcaccagcc
     2461 caacaagcac cggcaccgag gtgcgtttta cgacttcaaa tggtgagatt ttcgccatcc
     2521 cggcaaccgc aacgactacg ccagaaacgg gtgacagggt acggccgagg tttgacgcct
     2581 gcagcattgg gatagtcaaa tacgccgggt taatgccgga agagtgcgcc agtttcggga
     2641 tcatctcaac aaacgcataa aacggcgcat tgcctgaacc ggtcgtgact gccgccagca
     2701 ttgtcaggat caccaatacc agcatcagga tgatgctcgc cgaaccaaac gaggtagcga
     2761 tagaaatcag actttgaata aagccgatgg tgctaagccc ctgagcgaat accccagcgg
     2821 caaccagcag catcaccacg tttgcaaatg catctgccat cccgcgataa gccacttcca
     2881 gaccagagaa aactttctgg gtattaaagc tgcggatgaa ctccagaatg gaggcaatca
     2941 gcatacaaat caccagaata gtgatgatgt gtaattgcgg accccatttg ccgtcaaaaa
     3001 tcagcactcc gatgatcggc gtgaacggca aaatggcata aaacgcaggg gcagtggtgg
     3061 tgatttcact gacatctaac atttcatgag agatgtgttc ttttttatcc agataacgtt
     3121 gccagaagaa gtgggcgatt gccatgccga taattgcagc aattgagata ggcagcgttg
     3181 ttttgaaggc gaagtcaatc agcgacattt cggaagcctg cgccgccagc accacatccc
     3241 ctgaagtcgg tgcgagaata atcgccgccg gggaggcaca aatagcagca gctgcaccac
     3301 ggctgatacc aacgtttacc atcaccggaa acagggttgc catcagcaaa acacccagac
     3361 cggtcgcgga agagacggct agagacatca gacaggcgac aaaataggcg gcaatcatca
     3421 gcaggtaagg ggagttaata tactgcaatg gttttgacgc caacttgacc accatatcat
     3481 tcgcgccgat atgggtcatg taagcggcaa agccacacag catcataatc atcatgccga
     3541 ggtcgccgcc gcggctcatt agcaatattt taacgtattc aacgatatcc gtggcgctgt
     3601 agcctgttga agcctggctg gacggtaaca ctttgtgccc cataatggca ctgataatca
     3661 gcaataacag gccaccgaca aataacacgc cagtggcgga atacccttta atgatgtagc
     3721 gagctacacc cacaataacc acaaccccaa taaggagttc aatgaatgtc agcataattt
     3781 ttcctgtctc caggccccaa agtaaataat aaaaaattcc taaagcttaa ggaaaaaata
     3841 tgcccaataa attggcgatg aatgctgatt aaaatcaaga aaaactgcca ttaagacatt
     3901 gaagttgctg tttttataca tagataacaa aacgcatatt tagcggttgg caattatcac
     3961 ataataatgt gtgtaaattg atggtggatt gatgcaaatt tgttaattgc aatggtgtta
     4021 gctgcggctg tgcgctcaaa aaataatcta atatgagca
//
LOCUS       DAZUCC010000308         4056 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZUCC010000308 DAZUCC010000000
VERSION     DAZUCC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.310"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(218..865)
                     /gene="ychE"
                     /locus_tag="ACHY09_004795"
     CDS             complement(218..865)
                     /gene="ychE"
                     /locus_tag="ACHY09_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFS3887642.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(896..949)
                     /gene="ychE"
                     /locus_tag="ACHY09_004796"
     CDS             complement(896..949)
                     /gene="ychE"
                     /locus_tag="ACHY09_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFS3887643.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1342..4017
                     /gene="adhE"
                     /locus_tag="ACHY09_004797"
                     /gene_synonym="adhC"
     CDS             1342..4017
                     /gene="adhE"
                     /locus_tag="ACHY09_004797"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS3887644.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVAYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1124 a          998 c          948 g          986 t
ORIGIN      
        1 aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa aataacatga
       61 tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag aagaatattt
      121 tcacccattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata atgacgaagt
      181 aaacggagca tgacagctcc gtttcatttg aaaggaatta attaagcagg ccggggaaaa
      241 tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat agccccataa
      301 tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac ggtgccatgc
      361 ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa ccaaacagat
      421 agctaatgct gtgataacgc gtcccccaga cgatggtaga actgatcgcc cctggccccg
      481 ccatcaacgg caacgccaat ggcaccacac caatgctttc acgcaccgcg gtttctgatt
      541 tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac atcgctatcg
      601 tcaccaccag gatgccaccg gcgatacgga atgaatcaat tgatatacca aaaagttgta
      661 gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac aggttggctg
      721 taaggttcgt tttgtttcgc gctgccgctg tctgataact ggtcatgctg ataaagacgg
      781 gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg aagtaaacgg
      841 gaaaatcaaa aagggtctga atcacggtta gctccgaagc aaaagccgga taatgttagc
      901 cataaataag gttaaaaaga cgcgctgaca atacgccttt tgacagcatt tttcacctcc
      961 taactactta aaattgctat cattcgttat tgttatctgg ttgtgcaaaa catgctaatg
     1021 tagccaacaa atcatactac aatttattaa ctgttagcta taatggcgaa aagcgatgct
     1081 gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc cagaagtgag
     1141 taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta atgtttaaac
     1201 tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc atcggttaag
     1261 caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac taaaaaagtt
     1321 taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa cgcactcgta
     1381 gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca agtagacaaa
     1441 atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc gaaaatggcc
     1501 gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca ctttgcttct
     1561 gaatatatct ataacgccta taaagatgaa aaaacctgtg gtgttctgtc tgaagacgac
     1621 acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat cgttccgacc
     1681 actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac ccgtaacgcc
     1741 attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc tgatatcgtt
     1801 ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat cgatcaacct
     1861 tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat cctcgcgact
     1921 ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat tggtgtaggc
     1981 gcgggtaaca cgccagttgt tatcgatgaa actgctgata tcaaacgtgc agttgcatct
     2041 gtgctgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca gtctgttgtt
     2101 gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg cggctatctg
     2161 ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg tgcgctgaac
     2221 gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt ctctgtacca
     2281 gaaaatacca agattctgat cggtgaagtg accgttgttg atgaaagcga accgttcgca
     2341 catgaaaaac tgtccccgac tctggcaatg taccgtgcta aagatttcga agacgcggta
     2401 gaaaaagcag agaaactggt tgctatgggc ggcatcggtc atacctcttg cctgtacact
     2461 gaccaggata accaaccggc tcgcgttgcc tacttcggtc agaaaatgaa aacggcccgt
     2521 attctgatca acaccccggc gtctcagggt ggtatcggtg acctgtataa cttcaaactc
     2581 gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc tgaaaacgtt
     2641 ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa catgttgtgg
     2701 cacaaacttc cgaaatctat ctacttccgc cgtggctctc tgccaatcgc gctggatgaa
     2761 gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct gttcaacaat
     2821 ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac tgaagtcttc
     2881 ttcgaagtag aagctgaccc gaccctgagc atcgttcgta aaggtgcaga actggcaaac
     2941 tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga tgccgcgaag
     3001 atcatgtggg tgatgtacga gcatccagaa actcacttcg aagaactggc gctgcgcttt
     3061 atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc gaaaatgatc
     3121 gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt tgtaactgac
     3181 gacgctactg gtcagaaata tccgctggca gactatgcgc tgaccccgga tatggcgatt
     3241 gtcgatgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg tggcctggac
     3301 gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt ctctgatggt
     3361 caggcactgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta ccacgaagga
     3421 tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc gggtatcgcg
     3481 tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg ttcccagttc
     3541 catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg ctacaacgcg
     3601 aatgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca ggctcgccgt
     3661 cgttatgctg aaattgctga ccacctgggt ctgagcgcac cgggcgaccg tactgctgct
     3721 aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg tattccgaaa
     3781 tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga taaactgtct
     3841 gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct gatctccgag
     3901 ctgaaacaga ttctgctgga tacttactac ggtcgtgatt atgtagaagg cgaaactgca
     3961 gcgaagaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc cgcttaatca
     4021 gtagcgctgt ctggcaacat aaacggcccc ttctgg
//
LOCUS       DAZUCC010000309         4055 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZUCC010000309 DAZUCC010000000
VERSION     DAZUCC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.311"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            617..1489
                     /locus_tag="ACHY09_004798"
     CDS             617..1489
                     /locus_tag="ACHY09_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFS3887645.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRNT
                     VVSVARAVWNWIFF"
     gene            1816..>4055
                     /locus_tag="ACHY09_004799"
     CDS             1816..>4055
                     /locus_tag="ACHY09_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFS3887646.1"
                     /translation="MLMKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSL
                     AAVTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTG
                     GQWINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQW
                     VHGGGKAAGTIINLDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTT
                     INKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRD
                     GWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAF
                     SVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLA
                     DSGAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFIARGGS
                     LAGTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVEKSGSGTLTVSNT
                     TLIQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATW
                     NIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQ
                     NNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVIEAINGATTEEGAFVQGN
                     MLQAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGV
                     NGENNSV"
BASE COUNT         1013 a         1071 c         1116 g          855 t
ORIGIN      
        1 agatttttaa caggacactt cacattactg aacgctattc cgacagtatc actacacaaa
       61 taaaaaaggg aactggataa atattcatgc cggaagaatg gtgtaacaaa agcatttaat
      121 cattacgtgc gtattcctct ctattcgatc acggtttggc caaaccgtga tcgattcaga
      181 tgccattttg caagtacgca tgacccggaa gggaaacact taacaacatg gtatcaacag
      241 atatcattac gtgcgcactc ttataaaaat acacagcaga agcaagataa ctctaaacgt
      301 aaatttaata tctcccactg actgccccga caatatactg aaaaatactc acctcagtaa
      361 acataaacac acctgataat caggctgcaa gatgaatatt tttctccttt acggagtaat
      421 cagcctgctg tttaacaata accgcgtatt ttcatggaag gagatcaccc agtaacggat
      481 aagtcctgat ctccttcatg ttaatacgca acaatcccat cgattcaaaa caatttcaga
      541 cagatattat ctccgtggat cccccgccac ctttccggtg cgcggggttt tgtctttttt
      601 caccgggaat acatgtatga atccgtctga tgccattgag gcaattgaaa aaccgctttc
      661 ctccctgcct tactcacttt cccgtcacat cctggaacat ctgcgtaaac tcaccagtca
      721 cgaacccgtg attggcatta tgggtaaaag cggggccggt aaatcctcac tctgtaatgc
      781 actgtttcag ggggaggtca ccccggtcag tgatgttcac gccggcaccc gggaagtacg
      841 gcgcttccgt ctgagtggtc acggtcacag catggttatc actgacctgc ccggggtggg
      901 cgagagccgg gacagggatg cagagtatga ggccctgtac cgtgacattc tgcctgaact
      961 tgacctggtg ctgtggctga ttaaagccga tgaccgggcc ctgtctgtgg atgagtattt
     1021 ctggcgacac atcctgcagt gcggacatca gcaggtgctg tttgtggtga cgcaggccga
     1081 caaaacggag ccctgccatg aatgggatat ggccggtatt cagccttctc ctgcacaggc
     1141 acagaatatt cgcgaaaaaa cggaggcggt attccgtctg ttccggcccg tacatccggt
     1201 tgtggccgta tcggcccgca ccggctggga actggatacg ctggtcagtg cgctcatgac
     1261 agcgcttccc gaccatgccg ccagtcccct gatgacccga ctgcaggacg agctgcgcac
     1321 ggaatctgtc cggtctcagg cccgtgaaca gtttaccggt gcggtggacc ggatatttga
     1381 cacagcggag agcgtctgtg ttgcctctgt tgtccgtacg gccctgcgcg ctgttcgtaa
     1441 cacggtggtc tctgttgccc gtgcggtatg gaactggatt ttcttctgaa cctgtcgtga
     1501 ctgatgccct ccctgactct gagtctgctc acaaaagcgc tgttttcgtt actgtctctc
     1561 ttgtccgtgc aatagccaaa caatagaata aaacgatcga tacctatttt atcgatcgtt
     1621 tatatcgatc gataagctaa taataacctt tgtcagtaac atgcacagat acgtacagaa
     1681 agacattcag ggaacaacag agccacacgt cagaaacttc cgtcagccgg acctctggca
     1741 ctgtaaccct ttacctgccg gtatccacat ctgtggatac cggctttttt attcactctc
     1801 actctgatta aggaaatgct gatgaaacga catctgaata cctgctacag gctggtatgg
     1861 aatcacatta cgggcgcttt cgtggttgcc tccgaactgg cccgcgcacg gggtaaacgt
     1921 ggcggtgtgg cggttgcact gtctcttgcc gcggtcacgt cactcccggt gctggctgct
     1981 gacatcgttg tgcacccggg tgaaacagtg aatggcggaa cactggtaaa ccatgacaac
     2041 cagtttgtat ccggaacagc tgatggcgtg actgtcagta ccgggcttga gctggggccg
     2101 gacagtgacg aaaacaccgg cgggcaatgg ataaacgcgg gtggcacagg cagaaacacc
     2161 actgtcaccg caaatggtcg tcagattgtg caggcaggag gaactgccag tgatacggtt
     2221 attcgtgatg gcggagggca gagccttaac ggactggcgg tgaacaccac gctggataac
     2281 agaggtgagc agtgggtaca cgggggaggg aaagctgccg gtacaattat taacctggat
     2341 ggttaccaga ccataaaaca tggcggactg gcaaccggaa ccatcgtcaa caccggtgca
     2401 gaaggtggtc cggagtctga aaatgtgtcc agcggtcaga tggtcggagg gacggctgaa
     2461 tccaccacca tcaacaaaaa tggccggcag gttatctggt cttcggggat ggcacgggac
     2521 accctcattt acgccggtgg tgaccagacg gtacacggag aggcacataa cacccggctg
     2581 gaggggggta accagtatgt acacaacggt ggcacggcaa cagagacgct gataaaccgt
     2641 gatggctggc aggtgattaa ggaaggagga actgccgcgc ataccaccat caaccagaaa
     2701 ggaaagctgc aggtgaatgc cggcggtaaa gcgtctgatg tcacccagaa cacgggcggt
     2761 gcactggtta ccagtacagc tgcaaccgtc accggtacaa accgcctggg agcattctcc
     2821 gttgtggcgg gtaaggctga taatgtcgta ctggaaaatg gcggacgtct ggatgtgctg
     2881 agcggacaca cagccactaa tacccgtgtg gatgatggcg gaacgctgga tgtccgcaac
     2941 ggtggtgccg ccaccaccgt atccatgggg aatggcggtg tactgctggc cgattccggt
     3001 gccgctgtca gtggtacccg gagcgacgga acggcattcc gtatcggagg cggtcaggcg
     3061 gatgccctga tgctggaaaa aggcagttca ttcacgctga acgccggtga tacggccacg
     3121 gataccacgg taaatggcgg actgttcatc gccagagggg gctcgctggc gggcaccacc
     3181 acgctgaata acggtgccac gcttaccctt tccgggaaaa cggtgaataa cgataccctg
     3241 accatccatg aaggtgatgc actcctgcag ggaggcgctc ttaccggtaa cggcagggtg
     3301 gaaaaatcag gaagtggcac actcactgtc agcaacacca cactcatcca gaaaaccgtc
     3361 aacctgaatg aaggcacgct gacgctgaac gacagtaccg tcaccacgga tgtcatcgct
     3421 cagcgcggca cggccctgaa gctgaccggc agcacagtgc tgaacggtgc cattgacccc
     3481 acgaatgtca ccctgacatc cggtgccacc tggaatatcc ccgataacgc cacggttcag
     3541 tcggtggtgg atgacctcag ccatgccgga cagattcatt tcacctccgc ccgcacaggg
     3601 aagttcgttc cgacaaccct gcaggtgaaa aacctgaacg ggcagaatgg caccatcagc
     3661 ctgcgtgtac gcccggatat ggcgcagaac aatgctgaca gactggtcat tgacggtggc
     3721 agggcaaccg gaaaaaccat cctgaatctg gtgaacgccg gcaacagtgg tacggggctg
     3781 gcgaccaccg gtaagggtat tcaggtgatt gaggccatta acggtgccac cacggaggaa
     3841 ggggcttttg tccaggggaa tatgctgcag gccggggcct ttaactacac cctcaaccgg
     3901 gacagtgatg agagctggta tctgcgcagt gaagaacgtt atcgtgctga agtccccctg
     3961 tacgcctcca tgctgacaca ggcaatggac tatgaccgga ttctggcagg ctcccgcagc
     4021 catcagaccg gtgtaaacgg tgaaaataac agcgt
//
LOCUS       DAZUCC010000310         4033 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZUCC010000310 DAZUCC010000000
VERSION     DAZUCC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.312"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..463
                     /locus_tag="ACHY09_004800"
     CDS             11..463
                     /locus_tag="ACHY09_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFS3887647.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTFIPERLHKT"
     gene            616..1890
                     /locus_tag="ACHY09_004801"
     CDS             616..1890
                     /locus_tag="ACHY09_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS3887648.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            1883..3984
                     /gene="mchF"
                     /locus_tag="ACHY09_004802"
                     /pseudo
     CDS             1883..3984
                     /gene="mchF"
                     /locus_tag="ACHY09_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
BASE COUNT         1149 a          759 c         1008 g         1117 t
ORIGIN      
        1 aatagtaatc atgtcttata taagggaaac catcagagga aaagatgaat ggactgttta
       61 tgaacagatc ggttttgcgg tcagttgtat gctctacaat cgtaattaca gtctgtatcc
      121 ggtgttaacc attcaatact ggactgaata tgcgatacag cataatcaga ttaaattcct
      181 gtttgattca cgaggttttc cactggcgta tattacctgg gcatatcttg aggctgatac
      241 agaagcgcgc ctgctcaggg atccagaatt caggttgcat ccgtctgaat ggaatgaaga
      301 tggaaggatt tggattctgg atttctgttg taaaccaggc tttggtcgaa aagttattga
      361 ctatctcata cagcttcagc catggggaga aggagaagta cgatggttaa gcaggcgaaa
      421 gaaaattgtg acattcatcc ctgagcggct gcataaaacg tagtacctct gaagatacag
      481 agataattgt aaattacggg gtaaatgcat cgctgatact attttgacag gactctgtat
      541 tttctggctt tgattatagc atttcgctta accagtgaag gtattacagg aaaagtgtga
      601 gtaagaggag ctattttgtt tcgtcaggat gctttagaaa acagaaaaat gaagtggcag
      661 ggacgggcaa tattacttcc cggaatacca ctatggttaa tcatgctggg aagcatagtg
      721 tttattacgg catttctgat gttcattatt gttggtacct atagccgccg tgttaatgtc
      781 agtggtgagg tcacaacctg gccaagagct gtcaatatat attcaggtgt acagggattt
      841 gttgtcaggc agtttgttca tgaaggacag ttgataaaaa aaggggaccc tgtttatctg
      901 attgacatca gtaaaagtac acgtagtggt attgtcactg ataatcatcg tcgggatata
      961 gaaaaccagc tggttcgtgt ggacaacatt atttcccgtc tggaagaaag taaaaaaata
     1021 acgctagata ccctggaaaa acaacgcctg caatacacag atgcgtttcg tcgctcatca
     1081 gatattatac agcgtgcaga ggaagggata aaaataatga aaaacaatat ggagaattac
     1141 agaaactatc aggcaaaagg gctgattaat aaagatcagt taactaacca ggtggcatta
     1201 tattatcagc aacaaaacaa tcttctcagc ctgagcggac agaacgaaca gaatgctctg
     1261 cagataacca ctctggagag tcagattcag actcaggctg cagattttga taaccgtatc
     1321 taccagatgg aactgcaacg gtacgagtta cagaaagaac tggttaacac tgatgtggag
     1381 ggcgaaatca ttatccgggc gttgactgac gggaaagttg actccctgag tgtcactgtc
     1441 gggcaaatgg tcaataccgg agacagcctt ctgcaggtta ttcctgagaa cattgaaaac
     1501 tattatctta ttctctgggt cccgaatgat gctgttcctt atatttcggc tggtgacaaa
     1561 gtgaatattc gttatgaagc ctttccggca gaaaaatttg ggcagttctc tgctacggtt
     1621 aaaactatat ccaggactcc tgcgtcaaca caggaaatgt tgacctataa gggagcacca
     1681 cagaatacgc cgggcgactc tgttccctgg tataaagtca ttgcgatgcc tgaaaagcag
     1741 attatcagat atgacgaaaa atacctccct ctggaaaatg gaatgaaagc cgaaagtaca
     1801 ctatttctgg aaaaaaggcg tatttaccag tggatgcttt ctcctttcta tgacatgaaa
     1861 cacagtgcaa caggaccgct caatgactaa cgggaatttc agacaaatta taaatcagct
     1921 tgatatgcgc tggcgacgtc gtgttccggt tattcatcag acggagaccg ctgaatgtgg
     1981 actggcctgc ctggcaatga tatgcggtca ttttggtaag aatattgacc tgatatctct
     2041 tcgccggaag tttaatctct cggcccgtgg agcaaacctt gcaggaatca atggaatagc
     2101 ggagcagctg gggatggtca cccgggctct ttcactggag ctggatgaac ttggtgccct
     2161 caaaatgccg tgtattctcc actgggattt cagtcacttc gtcgtgctgg tcagcgtaaa
     2221 gcgtaaccgt tatgtactgc atgatccggc cagaggcaga agatatctcg gtcgggagga
     2281 aatgagccgg tattttacgg gcattgcact tgaggtctgg cccggaagtg aattcctggc
     2341 ggaaacccag cagacccgca taagtctccg ttcactgatt aacagtattt acggtattaa
     2401 aagaacactg gcgaaaattt tctgtctgtc agttgtaatt gaagcaatca atctggtaat
     2461 gccggtggga actcagctgg ttatggatca tgcgattccg gcgggggaca gagggctgct
     2521 gacgcttatt tctgctggcc tgatgttctt tatattgctc agggccgcgg tgagtatgct
     2581 gcgtgcatgg tcctcactgg ttatgagcac gctcatcaat atacagtggc agtcgggtct
     2641 gtttaaccat cttctcagac tgccgctggc gttttttgaa cgccgtaaat taggtgatat
     2701 ccagtcgcgt tttggctccc ttgacacttt gagggccacc tttaccacct gtgttgttgg
     2761 ggcaatcatg gacagtatta tggttgtggg tgtttttgtg atgatgctgt tatatggagg
     2821 atatcttacc tggatagtgc tcggttttac catggtttac gttcttattc gtctggtgac
     2881 atacggctat taccggcaaa tatcggaaga aactcttgtc agggggcccg ggccagctcc
     2941 tattttatgg aaagcctgta tggtattgcc acggtaaaaa tccagggtat ggctgggatt
     3001 cggggaacac actggcttaa cctgaaaata gatgcgatca attcaggtat taagttaacc
     3061 aggatggatt tgctcttcgg ggggataaat acttttgttg ccgcctgtga tcaggtggcg
     3121 attttatggc tgggtgcaag ccttgtgatc gataatcaga tgacaatagg aatgtttgtg
     3181 gcatttggtt cttttcgtgg gcagttttcg gatcgggttg cttcgctgac cagttttctt
     3241 cttcaactga gaataatgag tctgcataat gagctcattg cagatattgc actacatgaa
     3301 aaggaagaaa agaaaccgga aattgaaatc gttgctgaca tgagcccggt ttcactggaa
     3361 accactgatt taagctaccg gtatgacagc cagtcagcac aggtattcag tggtctgaat
     3421 ttgtctgtgg ctccgggaga aagtgtggct ataactggtg cctccggtgc cggaaaaacc
     3481 acattaatga aagtattatg tggactgttt gaaccagata gtggaaaagt actggttaat
     3541 ggcacggata tacgtcaact tggaataaat aattatcacc gtatgatagc ctgtgttatg
     3601 caggacgacc gactattttc aggatcaatt cgtgaaaata tctgtgggtt tgcagaagaa
     3661 acagacgacg aatggatgac agaatgtgcc aaagcaagtc atattcatga tgtgataatg
     3721 aaaatgccaa tggggtatga aacgttaata ggtgaactgg gggaaggtct ttccggcggt
     3781 caaaaacagc gtatattcat tgcccgagct ttataccgga aacctggaat attatttatg
     3841 gatgaggcta caagttctct tgatacagaa agtgaacgtt tcgtgaatgc tgctataaaa
     3901 aagatgaata tcacccgggt gattattgca catagagaga caactttaag aacagttgat
     3961 cggattttta gaattacaaa ttaggataat atatcactaa aagcagcatt gctgcataat
     4021 aagctatgct gct
//
LOCUS       DAZUCC010000311         4001 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZUCC010000311 DAZUCC010000000
VERSION     DAZUCC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.313"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..480
                     /locus_tag="ACHY09_004803"
     CDS             <1..480
                     /locus_tag="ACHY09_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFS3887649.1"
                     /translation="PETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDA
                     TANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGLVTEMTDP
                     GDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
     gene            496..1833
                     /gene="tssK"
                     /locus_tag="ACHY09_004804"
     CDS             496..1833
                     /gene="tssK"
                     /locus_tag="ACHY09_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3887650.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCKDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            1830..2482
                     /gene="tssL"
                     /locus_tag="ACHY09_004805"
                     /pseudo
     CDS             1830..2482
                     /gene="tssL"
                     /locus_tag="ACHY09_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
     gene            2485..>4001
                     /locus_tag="ACHY09_004806"
     CDS             2485..>4001
                     /locus_tag="ACHY09_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3887651.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWYCLSSRRDAPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVR"
BASE COUNT          797 a         1026 c         1214 g          964 t
ORIGIN      
        1 ccggaaactc gggagtttga attcgctaat ctgggcttta ttccgctctc ttactacaag
       61 aaccgggatt atgcctgctt cttctccgct aactccaccc agaaaccggc actgtacgat
      121 acgccggatg caacggcgaa cagccgtatt aatgcccgtc ttccctatat cttcctgctg
      181 tcccgcattg cgcattacct gaagatcatt cagagggaga atatcggtac caccaaagat
      241 cgccgtctgc ttgaactgga actgaataac tggatccggg gtctggtgac ggagatgacc
      301 gatccgggtg atgaactgca ggcctcacat ccgctgcgtg acggaaaagt ggtggtggaa
      361 gacatcgaag acaacccggg atttttccgc gtgaaactct atgctgtacc acatttccag
      421 gtggaaggta tggacgtcag tctgtcactg gtttcgcaga tgccgaaggc aaaagcgtaa
      481 ggcaggcgtc gggaaatgaa gatttatcgt ccactgtggg aagacggggc ttttctgatg
      541 ccccagcagt ttcagcagca ggctgcctgg gatgttcatc tggcagacag cgttgcccga
      601 atggggctgg cgcatccctg gggcgtggtg gctgcagagt ttgatgactc tctgttaccg
      661 ctttcacggt tgaatgccac ccgtctgatt gttcgtttcc cggacggaac gcttattgat
      721 acggaacgtg cggataatct gcctccggtc tgtgatttat cgacggtttc tgagcgttcc
      781 ctggtggata ttgtgctggc gctaccgtta ctgaatgcta acggtggcaa ccttgatgat
      841 ggcagtgaga gcgagcgtcc gcgtcgctgg aaatcagaaa gggtgaatgt gcaggagctg
      901 gccgggcatg agcaaagcga agtggcggta ttgcggcata acctgactct gcgcatggct
      961 catcaggaaa atgctgcctg gctgacctgt cctgttaccc gtcttgttcg agatgcacag
     1021 ggacagtggt gcaaggaccc gcgttttatt ccccctctgc tgacgctgtc tgccagtcct
     1081 tcgctgatga ctgagcttgc ggagttgctt catcacctgc aggcgcgtcg ccagcgactg
     1141 atgtccatgc gtcgggagaa caatgcccgt cttgcggatt ttgcagtggc ggacgtgtcg
     1201 ttgttctggt tgctgaatgc cctgaacagt gcggagcctg ttctgaaaga gttgcttgac
     1261 atgccttacc gccacccgga actgttgtac agggagcttg cccggctggc aggctccctg
     1321 ctcacattct cgcttgagca taatgtggat gcggttccgg cataccgtca tgaaacacct
     1381 gaaaatgtgt ttccaccctt gctttctttg ctgaaccagc tgctggaggc aagcctgcct
     1441 tcccgtgtgg ttttcattga actgaaacaa aatggtgtga tgtgggaggg ggcattacat
     1501 gatgcgcgtt tgcgcgaagg ggcggatttc tggctttcag tgcgctcttc catacccggg
     1561 catgaactgc agacgaagtt tccgcagcta tgcaaagccg gaagccccga tgatgtgtct
     1621 gaggtggtga atgtggcgct gagcggggtc attatccgtc cggttacgca tgtaccggcg
     1681 gccattccgc tccggctgga aaaccagtat ttcgcgctgg atttaagtac ggatgcagcc
     1741 cgggcgatgc tggacgcggg gcgttgtact ttctacaccc cggcatcgct gggggatgtg
     1801 aaactggaac tttttgcggt actgcggaca tgaataaacc tgttatctcc cgggctgaac
     1861 agatttttta tcccggctgg ctgatggtca gccagctgcg ctgcggacaa ccggttgagg
     1921 atggtaaggc cctgtatcgc cgtgcctgtc agctggtgac gcaggcgcgt gaagagctgg
     1981 ctgaagcaga attcagtcag gaaagcagtg aaatcatgtt gtatgccttc tgcgccctgc
     2041 tggacgagag tgtgctgaac cgtgaaaaaa cagacgatgg ctggcacacc tggcagcagg
     2101 acccgctgca ggctcatttt ttggtacgct caatgccgga gaagagctct gggagcgtat
     2161 tcgtgagcaa ctgaaattac ctgcgccgga tgtggcggta ctgacatgcc tctgccgtac
     2221 gcttcagctc ggttttaccg gtcagtaccg gtcgcaggat gatgagcgtc gcgaagatgt
     2281 aattcgcgca ctgacagcgc gtgttcctgc atttactttc gcacaggatg cgccggtggt
     2341 ggtgcgtgca ccgggttacc gtgctggcag aacgatgtac tggttgtcat gggtggcggg
     2401 tattgtgacg ctggctggtc tctggtgtgt gctttcttct gtgctcgcag accaggtggc
     2461 acggcttacc gggcagggat aaccatgcac gtacgttccc gtctctggct gacaatactg
     2521 agtctgttcc tgacactgtg gctgatttcg ggattcctgc cactgtctgc agtaaaccag
     2581 ataatactga ccctggtttc agttgtggcg gcgggactgg tgctgtggta ccagtggtat
     2641 tgtctgtctt cccgtcgtga tgccccggag aaggctggcg atgacggtct gccgcctgaa
     2701 tattttcagg gacatgtgct gcttgttgcc ggaaactcag atgcgtggtt cgaacccggt
     2761 caggcatacc gggaaacagc atcgggatgg tatcttcgtg tggatacgcc ggagcagatg
     2821 agaatactgg cagagcggtt gtcgtacagc cgtccggccc tgtgtggcca ggtcgtggtc
     2881 atgctgggac ttttaccgga gcagcatacc tcagatgaag ctttcctgtc gtggttgcgg
     2941 aactggcagc gtgcagtcat gcagtgccgg cagtggctga ataccatgcc gccggtgctg
     3001 gtgacattga tggtttcagg gccgtcacca caaaacacat ccttaaataa gcactctgca
     3061 cactggttta cactgacacc ggacctgtcg ggaatgcaca tacgcctgac ggaagccgga
     3121 gccattccgg cagaggaatg gctccgggag tgtgatgcag cgtccaggct ccggtgcgtc
     3181 agtgaactgt tatggctgaa tgccatgttg tcctggtaca accgggttac tgatgccctg
     3241 acagatattt gccggcagga gcacttttat ttccggccgg ttgccgttgg attctgtctg
     3301 acgtcggttg cggcccggcc cggtaatctc tggcaacaac aactgacgtc actcactgta
     3361 ctgccaccgc ctgccggcgt gtcatcacag gcactgccgt tgccggatat tctgctgtcc
     3421 ggtattcccc gtcatcgtgg actcagtcac tggcacagac tttgtctcca gacgggggcg
     3481 attgtcggcg tttttcttct gctggctatg ctggcgtctt ttatgaataa ccagcgactg
     3541 gttcgcagcg tgggggaaca cctgacggct tggcaccgga tgcgcggata ttcatctgaa
     3601 tcgaaaaaaa ctgcactgca acgtcttcag gctgacagcc agttactgaa cgactggcag
     3661 caacgtggtg tgccgcagcg ttacggactg ggattgtatc agggaatgtg gctgataccg
     3721 ccggttgagc gagcgactgg tgagtggatt gcaccactgc ctccccgtcc ggtaatacag
     3781 aaagttactc agacgccgaa aatggttcgc cttaacagcc tggcgctgtt tgatgccggt
     3841 aaatggacgc tgaaaccggg cgccacaaaa tggctggtga atgccctggt ggatattaag
     3901 gcaaaagcgg gctggctgat tgtggtcagt ggtcacacgg acaatacggg cgacccgcag
     3961 cgcaaccagg ccctgtcact gaagcgcgca gaggccgttc g
//
LOCUS       DAZUCC010000312         3985 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZUCC010000312 DAZUCC010000000
VERSION     DAZUCC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.314"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..435)
                     /locus_tag="ACHY09_004807"
                     /pseudo
     CDS             complement(1..435)
                     /locus_tag="ACHY09_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            743..856
                     /locus_tag="ACHY09_004808"
     CDS             743..856
                     /locus_tag="ACHY09_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS3887652.1"
                     /translation="MHDPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
     gene            complement(1100..1804)
                     /locus_tag="ACHY09_004809"
     CDS             complement(1100..1804)
                     /locus_tag="ACHY09_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HFS3887653.1"
                     /translation="MRRLPLMVGVIVGVMLAVGCHDRRAGVKTLLGDGMDQWGQKLPY
                     DHWQFNFFYPKNLPALVTMVYLEDGDVRESIFRQLDAARPSQSSVDTWSQRVGSFSAS
                     FNIGKALPVRMTVCWDSVIDKKAYETEIWFSRDTWQQMLTAYPDTYKPGKTYYRDNMI
                     IGLAPGGAVRIWLEDNGEPVVLQHPARQFTLTGDDMLICKNVPNQIDFSYIKANGYDP
                     FIRDFIKEKPYPYGHW"
     gene            complement(1815..3506)
                     /locus_tag="ACHY09_004810"
     CDS             complement(1815..3506)
                     /locus_tag="ACHY09_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFS3887654.1"
                     /translation="MVEEDEKNVPGDTCYYRYEIIGDSTHRTSIHNSFKTIQCRRVPV
                     RGITLTVGVFFDGTGNNRANADDLRLAYAHCANLVDEERARACEKYEKRARAGLGNAS
                     WQGGHTNISRLFDLYQQSDELNDSETEAQVKAYISGIGTADGESDSILGKALGSSLIR
                     QFEGVVTKTDEALEKISAELTRFIKLNRDKVAIAKVQFDVFGFSRGAAAARHFANRVM
                     NQDAAIAAAIDKGTDLTGHHGKLAGEVRFLGLFDTVAAIGSLLNFYDVNGRSNPGVNL
                     ELRPSVAQKVFQISAMHECRYNFSLNSIAGMWPELALPGAHSDIGGGYNAGEDEISLL
                     TMPDFEVTRDSADEMQTEVYRKAEKMRQVLYSLPALKYLMPHGEVETKMISWPLVNQN
                     KARASIFEKKVGAAVFMTRRDVPNDWEKVSLRVMLDAAQDAGAVFTPILSSNKELALP
                     SELLPLCDKAIAQGRAVRRGAEPEGFTAEDMRTIGRYLHCSANWNIDSDLSLWVSPSS
                     GEVFLRNNYAPTDERSFVWPMAPGKGWVRTVWRMDDQQQWEERARVNADALDGLF"
BASE COUNT          899 a         1224 c          898 g          964 t
ORIGIN      
        1 gggcaacaac tctttcacgc ggttggctgg ccaggtgttg atgacactga tcacgtggcg
       61 tagccacgtt tccggctcga ttccgttaag tttgcagcta ccgatcaggc tgtacatcac
      121 tgccgcactc tcgcctcctc catcagagcc ggagaacatg tagttacgcc gccccagtgc
      181 aactacccgg agggcgtttt cacacagatt attgtcgatc tccacccagc cattgcggca
      241 gtattcgttc agggcgtccc attgcttcag cagataggtg aacgctttcg ccgtatccga
      301 gtgacgcgac agcgtgctca tctgcccctg gagccactca tacaacgact gcattagcgg
      361 taccgttctg gcttttctga ccgccagtcg ctcttctgcc ggactgccgc ggatctcaga
      421 ctcgatagcg tacagtgcct cgatagggct gcgtttggga aacgtacaat attgtacggt
      481 tgcgctattg cggtagggtg tactccaacg ctatacggta tttggcagac ctttagggac
      541 atgagataac ctaaatgcaa taagagaaaa gcaataactt aaaggcgata gctgatcacg
      601 atgaatcaat cagttactta tccgcatcga ttaaaaaatg ctcctaacgt acaataattc
      661 cgtttcccaa accgacccct ctcatagctc acgctgttgg tatctcagtt cggtgtaggt
      721 cgttcgctcc aagctgggct gtatgcacga cccccccgtt cagcccgacc gctgcgcctt
      781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttaacgc cactggcagc
      841 agccattggt aactaaacaa ggtggattcc gatattagag tcttgaagtg atcacccaac
      901 tacggctaca ctgaaaggac agtattcggt atctgcgctc caccaagcca gttaccacag
      961 tcgattagaa gaggtcgaag acttctaatc ggacggaaaa agccgccgca aggcggcttt
     1021 ttccgtttac aagccgaaga ttacgcgcag cttaaaagga tctcaagaag atcattttaa
     1081 ttttgctgat cgttgctccc taccagtgac catacggata gggcttttcc ttgataaaat
     1141 cgcggataaa tggatcgtaa ccattagcct tgatgtaact gaaatcaatc tggttgggaa
     1201 cgtttttgca tatcagcata tcatctcccg tcagagtgaa ctgccgggca ggatgttgga
     1261 gtacaacagg ctcgccatta tcctccagcc agatccgcac ggcccctccc ggtgccaatc
     1321 cgataatcat attgtcccga taatacgttt ttcccggctt ataggtatca ggataggcag
     1381 tcaacatctg ctgccacgta tcccggctga accagatctc cgtctcatat gcctttttat
     1441 cgataaccga atcccaacac accgtcatcc tgaccggcag tgccttgcca atgttaaaac
     1501 tggctgaaaa actgcctacg cgctggctcc atgtatccac actactctga cttggtctgg
     1561 ctgcatcaag ctgccgaaat atgctttccc gtacatcacc gtcctccaga tacaccattg
     1621 tcaccagtgc tggcaggttt ttcgggtaaa aaaaattaaa ctgccagtgg tcataaggca
     1681 gcttctgccc ccattgatcc ataccgtcgc ccaacagcgt cttcacgcca gccctacggt
     1741 catggcagcc gacagccagc attacgccca ctatcacgcc caccatcagc ggtaatcgtc
     1801 tcattcctgc ctcctcagaa cagtccgtcc agcgcatccg cgtttacccg cgcccgctct
     1861 tcccactgct gctggtcgtc catccgccac accgtccgca cccagccttt gcccggtgcc
     1921 atcggccaca cgaacgagcg ttcgtcagtc ggagcatagt tatttcgcag aaaaacttct
     1981 cccgaggacg gactcaccca caggctcaag tctgagtcga tgttccagtt cgccgagcaa
     2041 tgcaggtaac gcccgatggt ccgcatatcc tccgccgtga agccctcagg ctccgcgcca
     2101 cgccggacag cacgcccctg agctatggct ttgtcacaca ggggaagcag ttctgacggc
     2161 aacgccagct ctttgttact gctgagtatc ggagtaaaca cagccccggc gtcttgtgcc
     2221 gcatccagca tcacccgcaa actcaccttc tcccagtcgt tcggcacatc cctgcgcgtc
     2281 ataaacactg ccgcgcccac tttcttctca aaaatagacg cacgcgcctt gttctgattg
     2341 accagtggcc acgaaatcat tttcgtctcc acctcaccgt gcggcatcag gtacttcagc
     2401 gccggcagtg agtacagcac ctgacgcatc ttctccgcct tacggtacac ctccgtctgc
     2461 atttcatccg cgctgtcgcg cgtcacctca aaatccggca tcgtcagcag ggagatttca
     2521 tcctctcctg cgttatagcc cccgccaatg tccgagtgcg cccccggcag cgccagctcc
     2581 ggccacatcc ccgcgatgct gttcaggctg aagttatacc gacactcatg catcgcgcta
     2641 atctgaaaca ccttctgcgc cactgaagga cgcagttcga gattcacccc gggattgctg
     2701 cgtccgttaa cgtcgtagaa gttcaacagg ctcccgatag ccgccactgt gtcaaacagc
     2761 cctaaaaagc gcacctctcc cgccagtttc ccgtggtgcc cggtcaggtc tgtccctttg
     2821 tctattgccg ccgctatcgc cgcatcctgg ttcatcaccc ggttggcaaa atgccgtgca
     2881 gccgccgcgc cgcggctgaa tccgaacacg tcaaactgca ccttcgcaat cgccactttg
     2941 tcacgattaa gtttgataaa gcgggtcagt tctgcgctga tcttctccag tgcttcatcg
     3001 gtctttgtca ccaccccttc aaactgtcgg ataaggctgc tgcccagcgc tttacccagt
     3061 atcgaatcac tttctccgtc tgccgtcccg atgccgctga tataggcttt gacctgcgcc
     3121 tcagtctcgc tgtcgttaag ttcatcgctt tgctgataaa gatcgaacag ccgggaaata
     3181 ttagtgtggc cgccctgcca gcttgcatta cccagcccag ctctggcgcg cttttcatac
     3241 ttctcacatg ccctagcccg ctcctcgtca accagattgg cacagtgggc atatgccagc
     3301 cgtagatcgt ctgcgttcgc ccggttattc cccgtcccgt caaagaacac ccccaccgtc
     3361 agcgttatcc ccctgaccgg cacccgccga cactggatgg ttttaaaact gttgtgtatg
     3421 ctggttcgat gggtactgtc cccaataatt tcataccggt aataacaggt atcgcccggc
     3481 acattttttt catcttcttc gaccataaaa atccttattt atctgaaaga taaaatcagt
     3541 agcaaaatcc ttcgggactt ttttacatct gcctgttaga cgtatttacc ataatccctt
     3601 aattgtacgc accgcagaaa cgcgctcagc gcgattctgg cgcaaaaacc gcaaaaatag
     3661 atgttttccc ccgggtaaac cggaaaaatg cgtcaggatt gttttcatgg cactgcatga
     3721 ctatgcatga aactgaatgg cgatcggttt gggcgcgcct gatgcccata aggcgcattt
     3781 tcggacgttt tcagccctga tgagaagaaa tcagacagta gttacagacg agtcgtgagc
     3841 gattcacccc gggagtcatc agcgagtcat ccagtatttt tcctcgcgac tctctggcga
     3901 ctcgcttttt ctgaacacca gagagacagt gtgttgagtc atcgataaat caccgacgac
     3961 tcgttgccga atcatccagt agtcg
//
LOCUS       DAZUCC010000313         3875 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZUCC010000313 DAZUCC010000000
VERSION     DAZUCC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.315"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..564
                     /locus_tag="ACHY09_004811"
     CDS             <1..564
                     /locus_tag="ACHY09_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887655.1"
                     /translation="VLLFLYINKDNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMT
                     EDVIQRITTFFQTRPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNG
                     RQVLDEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKSKYPAAICLILG
                     FFLSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(805..1500)
                     /locus_tag="ACHY09_004812"
     CDS             complement(805..1500)
                     /locus_tag="ACHY09_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFS3887656.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERFTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1493..2920)
                     /locus_tag="ACHY09_004813"
     CDS             complement(1493..2920)
                     /locus_tag="ACHY09_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFS3887657.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2931..3650)
                     /locus_tag="ACHY09_004814"
     CDS             complement(2931..3650)
                     /locus_tag="ACHY09_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFS3887658.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGLKVKVMHIAEVLMSR"
BASE COUNT         1011 a         1025 c          818 g         1021 t
ORIGIN      
        1 gtgctgttgt ttctgtatat caataaagat aatataaaag tgagttacag tctaaaaata
       61 aaccagacaa caccaggcat acttgttagc tgtgatagca ataataattt tgcctgtcag
      121 accacaatga ctgaagatgt tattcagcga attactacat tttttcagac cagaccagat
      181 gtcaagaaca gagaaataaa gctggaatgg tcaggagata agagagattt accaactgct
      241 gaagcggaaa tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc agaataccat
      301 aatggcaggc aagttcttga tgagatacaa acgccttcag caattaacag tgagctttat
      361 acaaaaatga tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta
      421 accataagct caccggaaat aaaaagtaaa taccctgctg ccatttgtct gattctggga
      481 ttttttctaa gcattgtgat ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa
      541 taccaacaaa actctgggca gtaataaaca gcattctgac agagagtgcc tgtataaaac
      601 tatagtgaca atgaccggga agggagcccg tcttcaggaa tggattcctg tctttttcac
      661 tgtaaatatc ctggcattat taatgtcagg atatttacag aagagcagca taaccatgac
      721 ggaacggagt taagtcaggt tgtcattagc gctattcgca acctcgtgcc gccttcaggt
      781 ttatgtccag acttcatatc tctctcagca atcctcaata atcagataca ccgctttcac
      841 cggaccatga accccgacga ctttgataag ctcaatgtcc gccgttgaac tgggaccact
      901 gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat gcaatttttc
      961 tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa gagaagattc
     1021 cgggagcagg ctcaatgaac gcccgcgccc ggcggcggaa aaaagaacca cgcctcccga
     1081 ttcggttaaa ccatattcag catatacgac gcccacttta gcctgctctg cttgtgatat
     1141 gttctcaata cctttcgccg gatcccaaac aacggcattg cattcctgct gcaaacgttc
     1201 gctaatcccc aattcctcca acctcgtgtc accactaatc acgaccgact gatctcccag
     1261 ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca gttcacagcg
     1321 cgtcaacata acatcgctgg caaactggat aaacgcgtca cagcgctgct gttgggtaag
     1381 ttgggtaaac cgctcgttag catagttgtt aagcggcgca tcttctgctt gcggttcaag
     1441 tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc gattatccat
     1501 tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct ccgtcagctt
     1561 caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt ggtgttttgc
     1621 cgccattgat aaaccagctt gccgcatgag caccagccat catcccaact ttccacaatc
     1681 ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct tttgcggtga
     1741 tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc ggaatacgca
     1801 ccggacacac gctgtcacaa gcggtgcata aagagcaggc gtagggtaaa tctttaaaat
     1861 ctttatagcc gccaagtaac ggggaaatca ccgcaccaat tggccctgga taaatagagc
     1921 catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa gccccgcaac
     1981 gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc ccgttatcga
     2041 caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc ggtcctgtca
     2101 gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc atggtgatca
     2161 atacatctac ctctgcaaac gtgggagcaa tacgctccat tcccatcact gcaatatgcg
     2221 ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg cataccgaac
     2281 cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg aggaaatctt
     2341 cgcggatttt ttgccgaatg aataaggtca tcgcttcagg cgtttccggc ccctcatagc
     2401 ccagacgttc gtgtagcacc cgacggatct gatggcgatc tttatgaatt gccgggacca
     2461 caacatgaga gggcggatct tgatccagct ggagaatgta ttcacccaga tcggtttcaa
     2521 tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg gtcaccatcg
     2581 atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa atgtagcggg
     2641 tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc actttttctg
     2701 agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc tgggcggccc
     2761 gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga tttgccccga
     2821 tacgctgctg cgcgtttgcc accgctttgc gcatgatcgg atcttcaatt tgctgacgga
     2881 tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc tcagcggctc
     2941 atcaacactt cagcaatatg catcactttg actttcagcc cttcccgttg taatcgtcca
     3001 ctgatgttca gcaggcaact cacgtcagca cctattaaat actcagggcg gacttccatc
     3061 aggtgcgcaa ccttttcttt caccatctcg ccggatattt cggccatttt gaccgagaac
     3121 gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc
     3181 acatttttca gcagcgtaag tggttcgtcc ttcaccccca gcttacgggc caggctacaa
     3241 gatgggtgat acaccgctct cccttgcaaa ctggcaccta catcgactac ccctaatgta
     3301 ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc
     3361 cattcaggtt catccgccag atacgtcggg taacttttta cggcataggt gcaagagcca
     3421 gctggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt tttcatccct
     3481 ggaatcgctt ctttgatata accactattg atcgcaggct gaccgcagca tccctgtttc
     3541 tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc tcgtgccatt
     3601 cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac aactaatact
     3661 ccattacttc atgcccattt atgcccgatg gttcccccct tccgggggat caatcatcaa
     3721 taaatatggc gttctttttt aattggctag caacatacca cccctcattt gtatgacaaa
     3781 tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt cggcatcgct
     3841 cacattttat agggaaatcg acgatcattt ttggc
//
LOCUS       DAZUCC010000314         3874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZUCC010000314 DAZUCC010000000
VERSION     DAZUCC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.316"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            192..1249
                     /gene="rfaQ"
                     /locus_tag="ACHY09_004815"
                     /pseudo
     CDS             192..1249
                     /gene="rfaQ"
                     /locus_tag="ACHY09_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
     gene            1246..2370
                     /locus_tag="ACHY09_004816"
     CDS             1246..2370
                     /locus_tag="ACHY09_004816"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS3887659.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQFSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2363..3160
                     /gene="rfaP"
                     /locus_tag="ACHY09_004817"
     CDS             2363..3160
                     /gene="rfaP"
                     /locus_tag="ACHY09_004817"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFS3887660.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKKEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVASLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3176..>3874
                     /locus_tag="ACHY09_004818"
     CDS             3176..>3874
                     /locus_tag="ACHY09_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS3887661.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVK"
BASE COUNT         1016 a          841 c          944 g         1073 t
ORIGIN      
        1 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa atcattcggg tcggattgcc
       61 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
      121 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
      181 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
      241 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaattacc ctgacgcaaa
      301 aatcgatgtg ctgctttatc aggacaccat cccgatcctg tctgaaaatc cagagattaa
      361 cgcgctctac ggcataaaaa ataaaaaagc aaaagcctca gaaaaaattg ccaacttttt
      421 ccatctcatc aaggtattac gtgccaataa gtatgacctt atcgttaatc ttaccgatca
      481 atggatggtt gctatactgg tgcgcttatt aaatgcccgt gtgaaaattt cccaggatta
      541 tcatcatcgg cagtctgctt tttggcgtaa aagtttcacc catttggtgc cgttgcaggg
      601 tggaaatgtg gtggaaagta acttatccgt gctgacacca ctgggagttg attcgttggt
      661 gaagcagaca accatgagtt acccgcctgc aagctggaaa cgtatgcgtc gcgaacttga
      721 tcacgctggt gttggacaaa attatgtggt tatccaacct acggcgcggc aaatcttcaa
      781 atgctgggac aacgccaagt tttccgctgt gattgatgcc ttacatgctc gtggttatga
      841 agtcgttctg acgtccggcc cagataaaga cgatctggcc tgcgtcaatg aaattgcgca
      901 gggatgccag acgccaccag taacggcgct ggccggaaag gtgaccttcc cggaacttgg
      961 tgcgttaatc gatcatgcgc agctgtttat tggcgttgac tccgcaccgg cgcatattgc
     1021 cgctgcagtt aatacgccgc tgatatcgct gtttggtgcg acagaccata ttttctggcg
     1081 tccctggtca aataacatga ttcaattctg ggcgggagat taccgggaaa tgccaacgcg
     1141 cgatcagcgt gaccgaaatg agatgtatct ttcggttatt ccggcggcag atgtcattgc
     1201 tgctgtcgat aaattactgc cctcctccac gacaggtacg tcgttatgat cgttgctttt
     1261 tgtttatata aatattttcc ctttggcggt ttgcagcgcg attttatgcg tattgctcag
     1321 acagtcgccg cccgaggtca tcatgttcgg gtttataccc agtcgtggga aggcgaatgc
     1381 cctgatgtat ttgaactgat caaagtgccg gttaaatcgc ataccaatca cgggcgcaat
     1441 gcggagtatt ttgcctgggt gcaaaaacat ttacgcgaac atcccgtcga taaagtcgtt
     1501 ggattcaaca aaatgccggg gctggacgtt tattatgccg ctgatgtttg ttatgccgag
     1561 aaagtagcgc aggaaaaagg ctttttctat cgcctgacgt cacgttatcg ccattatgcc
     1621 gcctttgagc gggcaacctt cgaacagggc aagccgacac agctgctgat gctgacagat
     1681 aagcaaatcg ccgatttcca gaaacattat cagactgaag cggagcgttt tcatattctg
     1741 ccaccgggga tttatcctga tcgtaaatat agccagcagc cagctaatag ccgtgaaatc
     1801 ttccgtaaga agaatggaat aaccgaacaa caatatttat tgttgcaggt cggttcagac
     1861 ttcacgcgta aaggtgtcga tcgttccatt gaagcacttg cttcgttacc ggattcgctg
     1921 cgccacaaca cattgctata tgttgttggg caggataaac cgcgaaaatt tgaggcgctg
     1981 gcagaaaaac gcggcgtgcg cagtaatgtt cacttcttct cggggcgcaa cgatgtctcg
     2041 gaattaatgg cggcggcgga tttattactg catcctgcct accaggaagc ggcgggaatt
     2101 gtgctgctgg aagcgattac tgcaggatta ccggtactaa caactgccgt ttgtggctat
     2161 gcgcattata ttgtcgacgc caattgcggc gaggctattg ctgagccatt ccgccaggaa
     2221 acattgaatg agattttacg caaagcgtta acgcaatttt cattgcgcca ggcctgggcg
     2281 gaaaatgcgc gacattatgc tgatacacaa gatttataca gtctgccaga gaaagcggcg
     2341 gatatcataa cgggtggtct ggatggttga acttaaagag ccgtttgcca cgttatggcg
     2401 cggaaaagat ccttttgagg aagttaaaac tttgcagggt gaggtatttc gtgaactgga
     2461 aactcgccgt actctgcgct ttgaaatggc gggcaaaagc tattttctca aatggcatcg
     2521 cggcacgacc ctgaaagaga taatcaaaaa tttactctca ttgcggatgc cagtattagg
     2581 cgcagaccgc gaatggaatg cgattcatcg actgcgggat gtcggcgttg atactatgta
     2641 tggggtggca ttcggcgaaa aaggcattaa tccgctcacc agaacctcgt ttattataac
     2701 cgaagatctg acaccaacca tcagtctgga agattactgt gctgactggg cgactaaccc
     2761 tccagatgtt cgcgtaaagc gtatgcttat taagcgtgtc gcgacgatgg tgcgcgatat
     2821 gcatgctgcg ggcattaacc accgtgactg ttatatctgt catttcctgc tgcacttgcc
     2881 tttttccggt aagaaagagg agttaaaaat ttcggtaatt gatctgcacc gggcgcagct
     2941 tcgcacgcgc gttccacgtc gttggcgcga taaagatctt atagggcttt atttttcttc
     3001 gatgaatatt ggcctgactc agcgggatat ctggcggttt atgaaagtgt attttgtcgc
     3061 ctcgcttaaa gacattctca agcaggaaca aggactgctg tcgcaagcag aagcaaaagc
     3121 cacaaaaatc agggaaagaa cgattcgaaa atcgttgtaa tttccggaga gaaatatgag
     3181 tgcccactat tttaatccac aagagatgat caataagaca atcatcttcg atgaaaggcc
     3241 agcggcgtca gtggcatcat cattccatgt tgcttatggc attgataaaa actttctttt
     3301 tggttgtggt gtttcaatca cgtcagtttt gttacataac aacgacgtaa gttttgtttt
     3361 ccacgttttt attgatgata tccctgaagc cgatatccag cgtttagccc aattggcgaa
     3421 aagctatcgt acctgtatcc agatccatct agtaaattgt gaacggctta aggcattacc
     3481 gacgaccaaa aattggtcta ttgccatgta tttccgtttt gtaattgcag attactttat
     3541 tgatcaacaa gataagatcc tttacctgga tgctgatatc gcctgtcagg gaaacttaaa
     3601 gccgctgata acaatggatc ttgccaataa cgttgctgct gttgttactg aacgcgatgc
     3661 taactggtgg tcgttacggg gtcaaagtct gcagtgtaat gaacttgaaa agggttactt
     3721 taattcaggt gtcctgttaa ttaatacact agcgtgggcg caggagtccg tttctgctaa
     3781 agcgatgtcg atgcttgctg ataaagccat cgtttcccgt ttaacctata tggatcaaga
     3841 tatccttaat cttatcctgt tagggaaagt taaa
//
LOCUS       DAZUCC010000315         3836 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZUCC010000315 DAZUCC010000000
VERSION     DAZUCC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.317"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1854)
                     /gene="rsxC"
                     /locus_tag="ACHY09_004819"
     CDS             complement(<1..1854)
                     /gene="rsxC"
                     /locus_tag="ACHY09_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFS3887662.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDP"
     gene            complement(1847..2425)
                     /gene="rsxB"
                     /locus_tag="ACHY09_004820"
     CDS             complement(1847..2425)
                     /gene="rsxB"
                     /locus_tag="ACHY09_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFS3887663.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISYNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2425..3006)
                     /gene="rsxA"
                     /locus_tag="ACHY09_004821"
     CDS             complement(2425..3006)
                     /gene="rsxA"
                     /locus_tag="ACHY09_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFS3887664.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFFQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(3083..3523)
                     /gene="ydgK"
                     /locus_tag="ACHY09_004822"
     CDS             complement(3083..3523)
                     /gene="ydgK"
                     /locus_tag="ACHY09_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFS3887665.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(3609..3824)
                     /gene="ydgT"
                     /locus_tag="ACHY09_004823"
     CDS             complement(3609..3824)
                     /gene="ydgT"
                     /locus_tag="ACHY09_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFS3887666.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT          964 a         1027 c          988 g          857 t
ORIGIN      
        1 cggatcgacc tgttgttctg gctcggcatt agcctgttgc tgctccagct tgcgcgcttt
       61 ggcacgggca atagcggctt cgacggcggc tttgcgcgga tcgacctgtt cttctggttc
      121 cgcattagcc tgttgctgtt ccagcttgcg cgctttggcg cgggcgatag cggcttcaac
      181 ggcagtttta cgtggatcag caacggttgc tgcgtcgtta gtttgctgca gttctgcctg
      241 tttcgctctg gcttgcgctt tacgggcttc ccgtgctgca ataattgcac tgttatccgg
      301 gcgttcgccc gctttaatca caataggctg tgtagcctgg gcctgtttct ctttcacccg
      361 cgccagagcg gcagcaatcg catctttatc tttggctgca ggttgaacgg ctgcgctctt
      421 atgtcgttca aggcgagccg ctttttcgcg ctccagacga gcctggcgcg cttcgaaacg
      481 cgctttggct tctgcggcgc gcttttcttc ctgacgaata gctgcaattt cagctttttc
      541 ctgacggaaa tattgcacca ggggaatatt gctcgggcaa acccacgcgc aagccccaca
      601 ttcaatgcag tcggcaatgt tatgcgtggt agctttatcg tgttgctgac ctttgctgaa
      661 ccagtacaac tgttgcggca aaagatccgc agggcaggcg tcagcacagg cgctacaccg
      721 gatgcagctt tgttcttcct gtggttcgcc aagctcattg gcagagggag ccaacagaca
      781 gttggtaatc tttacgaccg ggacatccag ccatggcaag gtaaagccca ttagcgggcc
      841 gcccataatc accatttgat cggcagaggg gcagaatccg gcgtcattca ataaatgacg
      901 cactggcgtc cccagccgtg cccagacgtt gcccgggcga gcgattgctt cgccagtcag
      961 ggttacgaca cgctcggtaa tcggctcgcc atcaataacg gcacgtttca ctgcataagc
     1021 agtgccgacg ttttgcatta atacgccgat atcggatgaa cgcccgccat gtggaacctg
     1081 cttcccggtc agaatgtagg ttaattgttt agcaccgcca gaaggatatt tggttggaat
     1141 cacccgcaga gaaatatcgt gagagtccgc cagcaccgcg cgcagcatgg aaatcgcctg
     1201 cggtttgtta tcttcaatgc cgataagaat ttcgcgtggc tgcagaatat gcgcaagaat
     1261 gcgaataccc tctacgacct gagccgcgca atcctgcatc aaacggtcat cggcggtaat
     1321 gtacggctcg cactcagcag cgttgataat caacgtttca atcttatctc cgccaccctg
     1381 caatttaacg cctgtcggga atcctgcacc gcccagcccg gcaacaccaa actgatgaat
     1441 gcgctcgatt aactcttcgc gactgcgaga gcgataatcg gcccagccgt ctcgcgggat
     1501 ccagcagtct tcaccatcgg catcaataat cacgcttaat tcagctaacg ctgaaggatg
     1561 agccgtagag tggggcgcaa tagccgtaac ggtacccgag gtgggcgcgt gaacaggcag
     1621 cattttgccg cgaccacggg taagcggctg gccgcgcaat actttatcgc cgacgctaac
     1681 gcacaactca ccttcagcgc caatatgctg tttcagtgga ataacaaaac gctgcgccag
     1741 tggtacctgg cgcaggggtg taccgttgga ctgggttttc atctccggtg gatggatgcc
     1801 gccgttgaaa tcccagattt tattttttct gaatgcagag aataacttaa gcatggtgtt
     1861 ccacgggaat gatacgcacg ggaatggtgt tcagatccca tttccaggag tcaggtgttt
     1921 ctgcgaccgg ttgcaacgag atgcagtgcg tcgggcacgg atcaacacat aaattgcagc
     1981 ccgtacagag atcactcatt accgtatgca tggcacgggt agcgccaacg atggcgtcta
     2041 ccggacaagc ctgaatacat ttagtgcagc caatacagtt attttcatca ataaccgcca
     2101 ccatccgcgc aggcgttagc tcttgcgctt cgccatccag cggctgcggc tcgacattaa
     2161 gcaactcggc aatttttagc atcacagctt cgccaccagg ggcgcaacgg ttgatttttt
     2221 caccgttata gctgatggct tccgcgtagg ggcgacagcc gggataaccg cactgaccac
     2281 actggctctg cggtaagatt tcgtcaattt tctcaacgac cggatcgtct tccaccgcaa
     2341 aacggcggga ggcataaccc agaatggcgc caaacgccag gcccagcagg ctcacggcgg
     2401 caacggcaat ccagatagca ttcattacaa cttcaccaaa ccactaaagc ccataaaggc
     2461 cagagacata agacctgcgg taattaatgc aatggcatta ccgcgaaaag gtgccgggac
     2521 atcagccaca gcaaggcgtt cgcggatggc ggcgaagagc accatcacca gcgagaaacc
     2581 gacagcggcg gaaaaaccgt acagcgccga ctgaaagaaa ttgtgcccga gattgatatt
     2641 cagcaacgcc acgccgagca ctgcacagtt ggtggtgata agcggcaaaa aaatccccag
     2701 cagacggtaa agcaccgggc tggttttgcg caccaccatc tcggtgaact gcacaaccac
     2761 agcaatcacc agaataaacg ccagggtgcg caggtaaatc agattaagcg ggatcaaaat
     2821 ccacgtatcg ataagccagg cgcaaataga cgccagcgtc atcacaaacg ttgtagccag
     2881 ccccatgccc atcgcggttt ccagcttttt ggaaaccccc ataaacggac agagaccgag
     2941 aaacttgacc agtacaaagt tattgaccag tacagttccg acaaacagta acaggtagtc
     3001 agtcattttt cggcctgaaa taaaaaagcc gcctattatc cgttaatcca gagcaggcga
     3061 caacaggtta actgtaaggt tattacggat tcacaaacgt cgctttcacc cgcgacgagc
     3121 gtttaaaata ggggacgatc aatgcggttg ccaacaaagt aaacagtaac tggcgaacgg
     3181 ctatgccatc ttccacgggt gaaaaagcga acgcttttac tgccagcaat acggaaataa
     3241 gcagccagat aatatagtgt ttaggaacac aacgccgacg tttgaagaaa gcgatggtca
     3301 accacagtgt gtaataccac atggcgatag cggtaataaa agaaacgccc cataagattt
     3361 gtgtcgtcag tgcttgttcg ccgagcgttt gaaatgtttg cggagaagat aacgcagcgg
     3421 tatacagcaa caacgccagc gtcgtactta acagagcgac taaaagccag gccaatgggc
     3481 caagcaacca gcctccaata cgttgtggcg ttgtggtggt cataggttct ccataatagc
     3541 ggcgcaaaca ggcaaatcta attttagggg cgttattata aggcttttat aaccgatcgc
     3601 tactttcttt attggacata gtgccagacg gacttcggta cctggccgag gtcaaacaaa
     3661 cgcccgccag aaaccagctc tgcgcgacgg tgatcggcag cacgatacat attgatcagt
     3721 tcctgatcgt ccgtcagggt gtaattaaga tggtcgtaga gtttttccaa actttcgagt
     3781 gaactgattt tgcgaaattt taataagtag tcctgaacag tcataaaagg tccata
//
LOCUS       DAZUCC010000316         3812 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZUCC010000316 DAZUCC010000000
VERSION     DAZUCC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.318"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(11..577)
                     /locus_tag="ACHY09_004824"
     CDS             complement(11..577)
                     /locus_tag="ACHY09_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS3887667.1"
                     /translation="MLIGYARVSTDDQNLDLQRDALQATGCGKIFEDQISGAKAERPG
                     IQAALHFARPGDTLVIWRLDRLSRSLKDLIEMVKHLESKGIGLKSLQESIDTTSSSGM
                     LIFHLFGALAEFERNLTRERTQAGLQAARARGRKGGRPKTLSKDKQALAVQLYNEKKH
                     TVAQICVLMGISRPTLYKYIESARLFKK"
     gene            748..3756
                     /locus_tag="ACHY09_004825"
     CDS             748..3756
                     /locus_tag="ACHY09_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS3887668.1"
                     /translation="MPADFLNAEQRVSYGQFSGEPNDVQLARFFLLDEADMEFISNRR
                     GRANRFAVALMIGCVRFLGTWPHDLSLIPANAQWFVARQLGISDTSVLADYSRRETTL
                     REHQALIRRQYGYRDFSWPWTFRLSRLLFTRCWLSNERPGLLFDLATRWLLQNKILLP
                     GVTTLTRLISQIREKSADRLWSRLSGLVSDAQNAQLEELLLVPEGARNSRFDQLRKGP
                     VAISGPAFNQAVARYLKLKAYGMQSIDFTGIPPVRFNTLAQYADIISVYKIARMSPAR
                     RTAMLVAFVRSCEISALDDALDVLDGVIADIGREAKKIGQKKRLRTLKDLDKSALELA
                     NICQMLLDENIDSELVRATIFERYPPSRLADTIGFINAIARPPDASFHDEMVEQYGRV
                     RRFLPCLLENIEFSATPAGETTLEAIRYLAAIRSTRRQHIDDAPMAIVTGPWKRLCYG
                     KDGHISRQGYTLCVMNKLQDCLRRRDIYVPQSGRWGDPRALLLQGQDWHASRVQVYRS
                     LGHSLNASEAVSALTQQLDTAYRQVAKNFADNQAVSLDFSGKRPKLTITNLNGLDESP
                     TLKSLAERVSGLLPVVDLTELLLEINAHTGFAEEFTHASEEGARMEDLTVSICAVLLA
                     EACNIGMEPFIRPNIPALTRHRLSWTRQNYLRAETLVKANARLVDYQSTLLLAQKWGG
                     GEVASADGMRFIAPVRTVHAGPNRKYFGSGRGLTWYNFISDQYSGFHGIVVPGTLRDS
                     IFVLEGLLEQQTGLNPTEIMTDTAGSSDLIFGLFWLLGYQFSPRLADAGASVFWRTDK
                     DADYGVLNDLARNTANTRKIEQHWDDMMRMAGSLTLGKIRASVAIRSLLSSERPSGLT
                     QAIIEAGKINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYQ
                     DGQEDQLGALGLVTNAVVLWNTIYMQAALDHLRNEGEPINEEDEARLSPLRHAHINML
                     GHYTFTLAEQVTKGHLRPLKQAEETDEWPL"
BASE COUNT          938 a          903 c          987 g          984 t
ORIGIN      
        1 tacgctaagt tcatttcttg aacaacctgg ctgactctat gtatttgtac agcgttggcc
       61 tcgatatccc catcaacaca caaatctgcg caacggtatg ttttttctcg ttatagagtt
      121 gaacagcaag ggcctgttta tccttactca gtgttttcgg cctgccgccc ttacgtcctc
      181 tggctcgtgc tgcttgaagc ccggcctgag ttctctctct tgtcaggttg cgttcaaatt
      241 ctgccagtgc cccaaaaaga tgaaatatca gcattccgga actggaggtt gtgtcgatgg
      301 attcctgaag gctttttaaa ccgatacctt tcgactccag atgtttaacc atttcaatca
      361 agtcttttag ggaacggctc agacgatcca gtcgccagat caccagggtg tctccggggc
      421 gggcaaagtg gagtgcagcc tgtatccctg ggcgttcagc tttggctcca ctgatctggt
      481 cttcgaatat ttttccgcac cctgtagcct gaagcgcatc ccgctgcaaa tccagattct
      541 ggtcatcggt tgagacccgg gcgtatccga tcagcatgat ttcatcccca aaatggcaat
      601 atactcgtta attataaacc aatgttaaca ttgatttctt tatcgggtat gttaacacca
      661 attattcagt cttctcgttt tgagtttgaa cgaagaacca tagtaaagaa aacgaccgtt
      721 ttctttactc atgccaggga ggctattttg cccgccgatt ttttaaatgc cgaacaacgg
      781 gtcagctatg gtcagttttc cggcgaacct aatgatgttc aactggcccg cttctttctg
      841 ttggatgaag cggatatgga gttcattagc aaccggcgcg gcagggcaaa ccgttttgcg
      901 gtggcattga tgatcgggtg tgttcgcttt cttggtacat ggcctcatga tctgtcatta
      961 attccggcta acgcacagtg gtttgtcgcc cgacaactgg ggatatccga tacaagtgtc
     1021 ctggccgatt attccagaag agaaaccaca ctgcgtgaac atcaggccct aatccggcgg
     1081 cagtatggtt atcgggattt ttcctggcca tggaccttcc ggctaagccg gttgctgttc
     1141 actcgatgct ggctcagcaa tgagcgtccg gggctacttt tcgatctggc gaccagatgg
     1201 cttctgcaaa acaaaatact gttgccaggc gtaaccacgc tgacccgtct gatatcacaa
     1261 attcgtgaaa aatcagcaga caggctctgg agcagactgt ccggactggt atcagatgcg
     1321 cagaatgcgc aactggaaga actgttactg gttcctgaag gtgcacgaaa ctcgcgtttt
     1381 gaccagttaa gaaaagggcc ggttgccatc agcggcccgg catttaacca ggcagttgcg
     1441 cgttatctga aacttaaagc atatgggatg cagtcgattg attttaccgg tatacctccg
     1501 gttcgcttca acactctggc gcaatatgca gacattattt cagtttataa aattgccaga
     1561 atgtctccag caagacgaac agcgatgctg gttgcatttg ttcgttcgtg tgaaatatca
     1621 gccctggatg atgcactgga cgttcttgat ggtgtcattg ctgatattgg gcgggaagcg
     1681 aaaaaaattg ggcagaaaaa acgactgcgg acactgaagg acctcgataa atctgcactg
     1741 gagctggcaa atatctgcca gatgcttctg gatgaaaata tcgacagtga attagtccgc
     1801 gcgacaatct ttgaaagata tcctccgtcc agactggccg atacaatcgg atttatcaac
     1861 gcgattgcga gacctcctga cgccagtttt cacgatgaaa tggtggagca gtacggaagg
     1921 gtacgccgtt ttcttccctg cttgttggaa aatattgaat tcagcgcgac accggcaggt
     1981 gaaacaacac tggaagctat tcgttacctt gcagcgatcc gctcaacccg gagacagcat
     2041 attgatgatg cgccaatggc gatagtaaca ggcccctgga aacgactctg ctatggcaag
     2101 gatggtcata tttcccgaca gggttatacc ctttgtgtta tgaataagtt acaggactgt
     2161 ttgcggcgac gggatattta tgttccgcag agcggtcgct ggggagaccc cagggccctg
     2221 cttcttcagg ggcaggactg gcacgccagc agagtacagg tttatcgttc ccttggacac
     2281 tctctcaatg caagtgaagc tgtcagtgct ctgacccaac agcttgatac tgcgtaccgg
     2341 caggttgcta aaaactttgc ggacaaccag gctgtttctc tggacttcag cggtaaacgc
     2401 ccaaagctca ccattactaa cctgaacggg ctggatgaat cacccacgct gaagtcgctc
     2461 gcggaacgcg tttctggcct gcttcctgtt gtcgacctga ctgagcttct tcttgaaatt
     2521 aatgcccata cagggtttgc agaggaattt actcatgcaa gtgaggaagg tgcacgaatg
     2581 gaggacctga cggtcagtat ttgcgcggta ttgctggccg aagcctgtaa tattggaatg
     2641 gaaccattta tccgaccgaa tattccggcg ctgacccgtc atcgtctgag ctggacccgg
     2701 cagaattatc tgcgggctga aacgctggta aaagctaacg ccaggctggt tgattatcag
     2761 tcaactcttc ttctggcaca gaaatggggt ggcggcgaag tggcctcagc ggatggcatg
     2821 aggtttattg caccggtacg aacagtacac gcagggccga accgaaaata ttttggttcc
     2881 ggccgcgggc tcacctggta caactttatt tcagatcagt attccggatt tcatggcatt
     2941 gtcgttcctg gtactctgag agattctata tttgtcctgg aaggactgct ggagcagcag
     3001 acggggttaa acccgacaga aataatgacc gatacggcag gttccagcga tctgatcttt
     3061 ggccttttct ggttactggg gtatcagttt tctccgcgtc tggctgacgc aggtgcttca
     3121 gtgttctggc gcacggataa agatgcagat tatggtgtac ttaatgatct tgcccgtaac
     3181 accgccaata cgcggaagat tgagcagcac tgggatgaca tgatgcgtat ggccggttcc
     3241 ctgacgttgg gaaaaatccg cgcttcagtg gcgatccgtt cattattgag cagtgaacga
     3301 ccttctggcc tgacgcaggc gattatcgaa gcggggaaaa taaataaaac gttgtatctg
     3361 cttaattata ttgatgatga agattatcgc cgccgcatcc tgacccaact caacagggga
     3421 gaaagccgac atgctgtagc cagagcgatt tgccacgggc aaaaaggtga aatcaggaaa
     3481 cgctatcagg atgggcagga ggatcagttg ggtgcgctgg ggctggtgac aaatgctgtc
     3541 gttctctgga ataccatcta tatgcaggct gcactggatc atttacgtaa tgaaggtgaa
     3601 cccataaatg aagaggatga agccagactt tcgccactga ggcatgcgca tatcaacatg
     3661 ctggggcatt acacgttcac acttgcggag caggtaacaa aaggccatct aaggccactg
     3721 aagcaagcag aagaaacgga tgaatggcct ctgtgacggt catattgtca cggtgcttaa
     3781 tgaccgtttg tttaaaaaac gtacttagcg ta
//
LOCUS       DAZUCC010000317         3809 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZUCC010000317 DAZUCC010000000
VERSION     DAZUCC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.319"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..334
                     /locus_tag="ACHY09_004826"
     CDS             <1..334
                     /locus_tag="ACHY09_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFS3887669.1"
                     /translation="AMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTWLQMN
                     AAVLRERKAAGMVVNVADESRLRVLRELAPGVVMVPASGSVLARRLQISHYPVLITDN
                     GLSQVPAP"
     gene            331..924
                     /locus_tag="ACHY09_004827"
     CDS             331..924
                     /locus_tag="ACHY09_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS3887670.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGRS"
     gene            921..1403
                     /locus_tag="ACHY09_004828"
     CDS             921..1403
                     /locus_tag="ACHY09_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887671.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRGSPAVMPWPDGIGISKVQERRAE
                     "
     gene            1400..2188
                     /locus_tag="ACHY09_004829"
     CDS             1400..2188
                     /locus_tag="ACHY09_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887672.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGERMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQAFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEAQLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEALSASPEKALPRSWWRRLLQRGTRS"
     gene            2185..>3809
                     /gene="traD"
                     /locus_tag="ACHY09_004830"
     CDS             2185..>3809
                     /gene="traD"
                     /locus_tag="ACHY09_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFS3887673.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTERSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSD"
BASE COUNT          777 a          909 c         1226 g          897 t
ORIGIN      
        1 ggcaatgtta ccggtcagta cgcctgaaat gtcacccgga gaggttgtgg cgcgccggct
       61 tcagttaccg ggcatcggtg cactttttct tgtgggcgat gatgcccatt cccgcacatg
      121 gctgcagatg aatgcagcag tactcaggga gcggaaggcg gcgggaatgg tagtgaatgt
      181 ggcggatgag agccgtctgc gcgtactgcg tgagctggca ccgggggtgg tgatggttcc
      241 ggcatccggt tctgtactgg cccgcaggtt acagataagt cattacccgg tgctgataac
      301 ggacaacggc ctgtcacagg tacctgcacc atgatgccgc tgtctgccac ttctgctgcg
      361 ctctctgctt ctccggtact gacagcctgc ctggttctca gcctgctggc tgttgtcctg
      421 gggccggcac tgtggcgcag ccggcgaaaa aaagcgtgtg tgagaacacg ccggcatcag
      481 cgttatcgtg ccacggcctc acgggtaatg aagcggctga acgaattgca gggtgatgcc
      541 cggcggctga aatatctgcg caaaatcagt ccgtatgtgt ttgaggaact gttgctgctg
      601 gcttttgagc aacaggggat gccggttatc cgtaacccct catacagcgg cgatggtggt
      661 cttgatggtc aggtggtgat taacggtgaa cgctggctga tacaggctaa acgttacgga
      721 agaaccattc gtccggcaca tattgatgct tttggcgaac tgctgcgccg gaagggatgc
      781 aggggctttt ttatccatac ggggcgtacg gggaacatca gccgggcacg gctgacactg
      841 tggccggata ttgagctgat cagtggacaa cgtctgttgc aactgctgaa tggcacctta
      901 ctgagtaata aaggaagatc atgattatga cactgtaccg gtttgttatg ctgctgctcc
      961 ggcttctgat tgtctggccg tcaatgctgg caggatgtct ggtaatgctg tatgtctgga
     1021 acaatctgag tggtggtgag gcaggcatga cacagcgtta tgcgacggag tcatcccgct
     1081 ggcgtgatgc gcctgaaggg catgtgatgg tacaaaaatg ttcctccgtc atatccgtct
     1141 caggtatgta tgtcgatgag caggaatgtc cgcgaacagc gatttctttc ggggaggctg
     1201 ttgctgaaga tattcgtgaa ctgaaaatgc aggcagtggt tctgttgctg ggcgctttgt
     1261 ttatggaagg tgtctcgtca gtcattctgt ttcgtggttg tggttctgca gccgtatccg
     1321 gggaaggcag ggggagcccg gctgttatgc cgtggcctga cggtatcggt atctcaaaag
     1381 tgcaggagag aagagctgaa tgaagggggc atttacagag cggatacagg cgaatgctcc
     1441 ggcctgggca catgaacaac tggttgcatg ggataccgca gagtttgcgg caatgtcgga
     1501 ttttattacg gaacactact ggacgggaca gggcagtatc aatgtgtacc gcattgtggg
     1561 gactgaccat ccacagtatg ccggaatgag ctggctggag ctgctggagc gtggtgaacg
     1621 catggacatc aatatcccgt tacttgaaaa gaatccggga tattacacgc aggcagagca
     1681 acagcatgcc gggatgggtt ttgtcagcac ggatggcatt cactggtatg tcagtgctga
     1741 cggtaatcac cgcagctgtc tggcccgctt tctgttccac ctgcaggggg aggggcgcac
     1801 acagcttcac aatgtggccc agagtattta tcacacagac caggcgttcc ggtctgcctg
     1861 tcgggaaatt cacaacctgg cagaaccgtt gtcccggcat gatgtgtatc ttcgtctgca
     1921 gatccggcgg cagtgtgttt cccgcgaaga cctggcctgc tggaaagtgg atcgtttcag
     1981 aacagaggcg cagttgacag tggatgatgt gcatgccggc ggacatgacg ggccactggt
     2041 gtataaggca ttactgctga atgcggcaga cgcatggcgg gaggtgatgg tgttacagcg
     2101 caggctggag gcactgtcag cgtcaccgga gaaagcgttg ccgcgctcat ggtggcggcg
     2161 tcttttacag agaggtaccc ggtcatgagt aaccgttatg tgattgaggc tctgctgcgt
     2221 ccggctgtgg aactgaatac cgccgtggtg gcagcaacgg cggccggtgt ctgtgtgaca
     2281 gcgccctggg cggtggcgct cgcgccttcg gtcagctatg tcacagccgc cggctttggc
     2341 gttctggcag tggtccgttt ccgacagggg atgaagatca tccgttaccg tcgcaatctg
     2401 cgccggctgc cccgttatgt gatgagttcc cggcaaattc cggtcagccg tcagcggctg
     2461 tttctgggac gcggcttccg ctggacacag aagcacaccc agcgccttca ggacacgctg
     2521 cgaccggagg ttgcgcacta tctgcagccg ggcagtctgt accggacagc gcgctggctt
     2581 gaaatgaaaa cggagcgcag tcttccctgg attgggcagc ttcttcgtcg tgactcgccg
     2641 ctgaatcccg tccgtcccct gccgccggtg ggaggaaatc ctgccctgca tggtattgag
     2701 ccggatgagc aggatgtgac gctggcactg ggtgaacgtg tggggcacac cattgtctat
     2761 ggcaccaccc gtgtcgggaa aacccggctg gcggagttgc tggtcacaca ggatattcgt
     2821 cggggagatg tcaccattgt ctttgatccc aaaggggatg cggatctgtt gctgcgtgtc
     2881 tgggctgaag cacaccgtgc cggcaggggc gatgagcttt atatctttca tctgggctgg
     2941 ccggaaatct ctgcgcgtta taacgccgtg gggcgttttg gtcgtgtgtc ggaggtggct
     3001 tcccgtgtgg ccggtcagtt atccggtgaa ggtaacagtg cggccttccg tgagtttgca
     3061 tggcggtttg tcaacattgt tgcccgtgca ctggtggcac tgggggagcg cccggattac
     3121 acgctgatta tgcgttatgt gaataatatt gccgaccttt atatccgtta tgctggtaag
     3181 gtgatcagcg aacagatgcc ggaactggaa aacgccattc tgaataacat gaatgtactg
     3241 ggggaagcgg atgttccccg gaccatgcag aaccagccgg atgctgtgcg tatctgggcc
     3301 attgaggtgg ctctcagcag tgaagccgga aagaaactct atgatccgat actggatggt
     3361 ctgcgaagcg cggtacgtta tgatcgtacc tattttgaca aaattgtggc atccctgttg
     3421 ccgttgctgg aaaaactgac cacaggaaag acggcagaac tgctggcacc tgactacctg
     3481 gatatgagcg atacccgtcc cgtgtttgac tgggagcagg tgatacgtaa aaaggcggtg
     3541 gtgtacattg gtcttgatgc gctgagtgac agtgaggtgg ccagtgccgt gggtaactcc
     3601 atgtttgcgg atctggtgag tgtcgccggg catatctaca aacacggaat gaatgccggt
     3661 cttccgggag ggaatggtac caaaaatccg gttaacctgc attgtgatga gtttaatgaa
     3721 ctgatgggcg atgagttcat tccgttaatc aacaagggag gcggtgccgg tatgcaggtg
     3781 acggcttaca cgcagacctc ttctgatat
//
LOCUS       DAZUCC010000318         3770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZUCC010000318 DAZUCC010000000
VERSION     DAZUCC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.320"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..327
                     /locus_tag="ACHY09_004831"
     CDS             112..327
                     /locus_tag="ACHY09_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3887674.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            501..758
                     /locus_tag="ACHY09_004832"
     CDS             501..758
                     /locus_tag="ACHY09_004832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887675.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            740..931
                     /locus_tag="ACHY09_004833"
     CDS             740..931
                     /locus_tag="ACHY09_004833"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3887676.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1073..2092
                     /locus_tag="ACHY09_004834"
     CDS             1073..2092
                     /locus_tag="ACHY09_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS3887677.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2813..3028
                     /locus_tag="ACHY09_004835"
                     /pseudo
     CDS             2813..3028
                     /locus_tag="ACHY09_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3203..>3770
                     /locus_tag="ACHY09_004836"
     CDS             3203..>3770
                     /locus_tag="ACHY09_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFS3887678.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWF"
BASE COUNT         1121 a          818 c          955 g          876 t
ORIGIN      
        1 tatagtaaca ggaacatcag taagcgatga gatggtcttc cccctccttc acgacaacct
       61 ttaattacgt catgatggat aaactttaac cacaaaagta agagggatac catgtctcaa
      121 cagaaaatca ccggcatcga tctggctaaa acgaactttt atcttttcag tatcaatgct
      181 catggcaaac ccgcaggaag gatcaaactt tctcgcaatc aactgcttaa ctggctggtg
      241 cagcagccta aaatgactgt tgcgatggag gctcgcgggc atcccatcac tgggccaggg
      301 aaatcaggaa actcgaccat gatgtaatat ttcttccggc gcaacatgtt aaagcttatc
      361 agcgacgcca gaaaaatgat tatgacgacg ctcaggcaat agctgaggcc tgccaacatg
      421 gaactatacg ccccgtacca ataaaaactc tggagtaaca ggacgtccag acttttttga
      481 aaatgcgccg gttggtttcg atggaaagga cacaactgat aaatcacgtt cgtggacttc
      541 ttgctgaata tggcattgtg ttcagtaaag gcgcaacaga attacgccaa aaattaccgg
      601 cattacttga ggatgcggaa aacgaactca ctgataccat gaagacgtta ttgcatcggc
      661 agtatatacg gttgattaca ctagataatg aactggagtg gtatgattct gaactgaaaa
      721 atatgtccgc caagatccta tgtgcaaacg cttgttagcc attcctagat tcagccctct
      781 ggttagtcag gcaataaaaa gctggatggg ggatggaaaa cagtttagac gaggaagaga
      841 tgcatctgcg actctgggac tggttccccg acagttcagc acaggtggga agcaggttct
      901 tctgggtata acaaaatttg gagaaatgta atgaacacat cccaccccat taagtcaggt
      961 gctctgtgat ggatgaaggg aaatactgga aggtgtggag cctcgcagat ttccggcatc
     1021 atagtgccca atacaggatg cataaagctc cctcagactg gagactccac aaatgaaata
     1081 tacaccggtt ggcgttgata tcgcaaaaca tctgattcag gttcatttca tcgacgagca
     1141 cacaggtgaa gtgattgata agcaactacg tcgtcaggat tttctgatgt tcttctgtaa
     1201 tcgggaaccc tgcctgattg gtatggaggc ctgtagaggc tctcagcact gggggcgtga
     1261 actgacaaag ctggggcata aagtccggtt gttacaggcc cactttgtta aggcattcgt
     1321 catgggaaat aagaatgatg tgatggatgc ccgggctatc tggatggctg ttcagcagcc
     1381 aggtaaggac atcgcggtta aaacagaaga acaacagtca gtgctggttt tgcaccgttc
     1441 ccgcagacaa ttggttaagt tccgcaccat gcagattaat tctctgcacg gcacgctcct
     1501 agaatttggt aaaaccattc acaaaggtcg ggcagcgatg gagcgagagc tccctgaagc
     1561 actggagcgg atgaaagaga gactgccgcc gtatctcatt actgtgctgg aaaaccagta
     1621 caaccggctg aatgagctgg actcgctgat agaggatatt gaaaaaaagc ttaccagcgt
     1681 ggcgagacag aatgaaacct gtaagcggtt gctggatatt cccggcgttg ggccacttat
     1741 tgcaacggca gcggtggcca ccatgaggga agcatcagcg tttaaatcag ggcgagagtt
     1801 cgcggcttat gttggtctgg tgcccaaaca gactggctca ggagggaaaa tccgcctgct
     1861 ggagataagc aaacgtggta atacatatct gcgagccttg tttattcata gcgcaagggc
     1921 ggcagcatta ctgcccaaag aacctggtcc gtggataacc gaactgaaaa aacgtcgtcc
     1981 aaccagcgtg gcaatcgtcg ccatggcaaa caaactagca cgaacagtat gggcgatagc
     2041 agctcatgga agtaaatacg acaagaatca tgtcagtatc agatcatatt aatcgctgat
     2101 accgataaat attaactttt aatgaaagga tgaatactga aaggtaactc tggcggccat
     2161 agtgatgaca acgacaggta agaccgggac tcactaaacc tgaatattgt gttgagctaa
     2221 aagctcgctg gagaaatgag gtgtgagtca gcgaattaca taggggctcg cagcttcatg
     2281 gctgcaaaaa agccggatat aaagctgcaa cctacccgtc actgtcaagg caatggatgc
     2341 cttgcaacgg gatgtgttca tataatcaat atgtcagatc tatgctgata cagggtgcca
     2401 gagcggtatt gttcagagcc tcagagaaaa ctgattcact tcgtgtgtgg gttaattgta
     2461 ttcgggaaaa gcgtggcttc aaccaggcag ttgttgctct tgcaaataaa ctccttcgga
     2521 tcgcctgggc aattatcgcc cgcaatgaga tgtatttgcc cgcagcaaaa caaaatcaat
     2581 aatctacgta aaatcagtag gagtaaagct taccctgaaa ttgcaaagaa tgcagaatga
     2641 tgaaaaacag gtcagaccga cgttacgaaa acctgcaaga tgagccaggc cagtaaggcc
     2701 gagtaagcga taaggtcgga gcgcgcgaat gtctcatcaa ggccagaagc aataacttca
     2761 tgaacaaacc ggatatacga aagtaaacct gtccttatca tagttgctgt actcttgcaa
     2821 tacgggggaa gaccatctca tcgcttacga acaattcgtg aaaacgatga agaaagacta
     2881 catcgcgtgc atgccgaaat ctaatgtaag aacggcattg cataatcttg cagtggagat
     2941 cgggtattac aatgaaaacc atccgcatag tgcgttaggt tatctcccac caagggaata
     3001 tcaacgtcag cgaataacgt caccttagat gcagtagttg tcaggaaata gtgactcaag
     3061 ggcaggctta ccatatacac tcttagtggt tcgactgggt attagaatag ctattaacag
     3121 attagcactg aacactgcgt aatagtggtt gcacaaacaa aatgttatgc tataacattg
     3181 ctgtattgac atatattttc gaatgtatat gaacgtaatc agaactgtca tttgtacatt
     3241 aatcatactt ccggtggggt tacaggcagc gacaagtcat tcttctatgg ctaaagatac
     3301 aatcaccgtt gtcgcgacag gaaatcagaa cacggtattt gaaacgccgt cgatggtcag
     3361 tgtcatcacg aatgacacgc catggagtca gaatgcggtc acatcagccg gtatgctgaa
     3421 aggtgttgcg ggtctcagcc agactggtgc aggacggacc aatgggcaga cctttaatct
     3481 acgcggctat gacaaaagcg gggtacttgt tcttgttgac ggcgtccgtc aactcagtga
     3541 catggcaaaa agcagtggca cttatctgga tccggcactc atcaaacgca tcgaagttgt
     3601 ccgcggacca aactccagtc tgtacggcag tggcgggctg gggggggtgg tggacttcag
     3661 aactgccgat gctgcagatt ttcttccccc cggagagaca aatggtgtaa gtctgtgggg
     3721 aaatatcgcc agtggtgacc acagcacagg ctcggggctc acctggttcg
//
LOCUS       DAZUCC010000319         3731 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZUCC010000319 DAZUCC010000000
VERSION     DAZUCC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.321"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..509
                     /locus_tag="ACHY09_004837"
                     /pseudo
     CDS             1..509
                     /locus_tag="ACHY09_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
     gene            750..1715
                     /gene="lipA"
                     /locus_tag="ACHY09_004838"
     CDS             750..1715
                     /gene="lipA"
                     /locus_tag="ACHY09_004838"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFS3887679.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1816..2019)
                     /gene="tatE"
                     /locus_tag="ACHY09_004839"
     CDS             complement(1816..2019)
                     /gene="tatE"
                     /locus_tag="ACHY09_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFS3887680.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2148..2936)
                     /locus_tag="ACHY09_004840"
     CDS             complement(2148..2936)
                     /locus_tag="ACHY09_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFS3887681.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARVLDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFALPQLL"
     gene            3029..3412
                     /gene="crcB"
                     /locus_tag="ACHY09_004841"
     CDS             3029..3412
                     /gene="crcB"
                     /locus_tag="ACHY09_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFS3887682.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3466..3675)
                     /gene="cspE"
                     /locus_tag="ACHY09_004842"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3466..3675)
                     /gene="cspE"
                     /locus_tag="ACHY09_004842"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFS3887683.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT          964 a          977 c          856 g          934 t
ORIGIN      
        1 caaattgaac cctgtttaag cagaaaatcg tcagaaggta aaccacaaat atttacaacc
       61 ttacgaaata ttgatcttac ccttctgact attttgaagt tgcatatgtc cataaaggaa
      121 tcgttaatgc agcgaaagtg cttaatctgc ccccctcggc aatctatcag cctattcaga
      181 aactgcgcgt tatattccct gacctattgt ttattcgcaa aggccagggt gtcactccta
      241 ccgcatttgc gatgcatcta tatgagtcta tcactcaggg acttgagtcc attcttggcg
      301 cgctggatat cgaaggaagc tatgataagc aacggacgat aactattgcg ccctccccct
      361 cggtcggggc ccaggtcctt cctgtcatct atcgggcgat taaaactcac tatccacagc
      421 ttttactgcg caacccgccc atcggcggcg cggaaaacca actctgtcag tttcaaaccg
      481 atctcatcac cgataacatg ttttgcacag gaccatagat gttatccgta atgcattttg
      541 aaaaagtatt gttacagcca gatcacagcg cagaacgata aaatggcaca atccaacaac
      601 aattttacat tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg atatactgcc
      661 ggtcgttaat tcaaaaatag ttgataatta caacaatcta ttgaattgaa acgctttcct
      721 tcgtaattcg caactggaac acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg
      781 ttaaataccg cgatgccgat aagatggccc ttatcccggt taaaaacgtg gcaacagagc
      841 gcgaagccct gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg gactctacac
      901 gtatccaggg catcaaagcc gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag
      961 cctcctgccc taacctggcg gaatgcttca accacggcac agcaacgttt atgatcctcg
     1021 gcgctatttg tacccgccgt tgtccgttct gtgacgttgc ccacggtcgc ccggtagctc
     1081 cggatgccaa tgaaccagtg aaactggcgc aaaccattgc cgatatggcg ctgcgttatg
     1141 tggttatcac ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccag cactttgcgg
     1201 attgcattac tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg
     1261 atttccgcgg tcgtatggat cgtgctctgg atattctgac cgccacacca ccagatgtgt
     1321 tcaaccataa cctggaaaac gtaccgcgta tttaccgtca ggtacggcct ggtgcagatt
     1381 acaactggtc gctgaagctg ctggaacgct ttaaagaagc gcatccggaa atcccgacca
     1441 agtctggtct gatggtgggt ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg
     1501 acctgcgccg tcatggtgtg acgatgctaa cgctggggca atatttgcag ccaagccgcc
     1561 atcacctgcc ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg aaagccgaag
     1621 cgctggcgat gggctttacc catgctgcat gcggtccgtt tgtccgctcg tcttaccacg
     1681 ccgatttgca ggcgaaaggg atggaagtta agtaatcctg taataattac ttacatctgt
     1741 ctgataaaaa acccgctttt tgaagcgggt tttttgtatc gaacaagata ttgagggagc
     1801 gtcctgctcg ccacgtcact ctttatgaga gagcttttca gcctgaagat caacgtctgc
     1861 gccttttttc gccgcagcat cgtcatcatt catcgccttc ttgaaccctt taatggccgc
     1921 tccaaggtct ccgcccagcg tacgtaactt cttagtccca aacagcagaa cgaccagcgc
     1981 cgcaactacc agcagtttgg taatactaat ctcacccata gataccttct tgacatataa
     2041 ccggcaacag aacgcgcctt aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca
     2101 caatagcaat ctgtaacaag gtgaatcaag cgccgagtaa aaaaacatca taataattgc
     2161 ggcagcgcaa agcgacggtt gtttaagacg ggcagttgcg cgcgtacctg acgcacacgt
     2221 tcgggcgtca cttccgccat aatgagtgca ggcatttctg acgctgccgc aatggtgacg
     2281 ccaaagggat ctataatccg gctttgcccg atatttttgt tcccgcactc ccccgccgcc
     2341 accatataac aggtggtatc cagcacacga gcggcaagca acgttgacca gtgatgctct
     2401 ttgagcggcc cacgaaccca ggcggcagga agtaccagga tttcagctcc ctgtaatgcc
     2461 tgtgccagcg ccagctctgg aaagcgtaag tcataacagg tcatcagacc gaccttcatc
     2521 ccttccacct ccagtaacgg agctatttca ttaccagcat caacatggcg tgattcctga
     2581 atggcaaatg catcatagag atgcagtttg gcataacggg cgacgatgtt tcctgcctgt
     2641 agcgccacca gcatattcca cgcgcgcccc ggtgttgaag gaacatgaat cgtcagaatt
     2701 gtcgtcatca tgttacgttt actttctcgc cgtaaacggt cgaggaattc gccttccagc
     2761 agctgtgctg atttaaccga aagatccgca tcatgatcat cacgcgccag taatgcttcc
     2821 ggcaggacaa acagcgaaac ctcgttttcc gccgcctgcg ccatcaacga ggcacaaatc
     2881 tcagcgttct tttcccacac agatgtaaca gcaaactgtc ctgcggcaac cagcattgcc
     2941 acctcctgtc agagtcgtaa aattcgccaa cggcgattat tcggcgttac acttatcact
     3001 catacaaatc aaatagcagg attttgcagt gttacaactt cttttagcag tttttattgg
     3061 cggtggtacg ggaagcgtgg cgagatggct gttaagtatg cgatttaacc cgctgcatca
     3121 ggcgattccg ttggggacgc tggctgcaaa cctgattggg gcattcatca taggaatggg
     3181 attcgcctgg ttcagcagga tgacgaacat tgatccagtg tggaaagtat taatcaccac
     3241 cggattttgt ggcggtctaa caaccttctc aacattttcg gcagaagtgg tgtttttgtt
     3301 acaagagggc cgctttggct gggcgttact gaacgttttc gtcaaccttt tagggtcttt
     3361 tgccatgacc gcactggctt tctggctgtt ttccgcctca accgcacact aaagaccaaa
     3421 aaaaacccgc tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac
     3481 gtttgcagca gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc
     3541 agcaagagtt ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct
     3601 gccgtcttcc ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc
     3661 tttaatctta gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgtgtcggt
     3721 taccagtaca c
//
LOCUS       DAZUCC010000320         3694 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZUCC010000320 DAZUCC010000000
VERSION     DAZUCC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.322"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..224
                     /locus_tag="ACHY09_004843"
     CDS             33..224
                     /locus_tag="ACHY09_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887684.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            588..839
                     /locus_tag="ACHY09_004844"
     CDS             588..839
                     /locus_tag="ACHY09_004844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887685.1"
                     /translation="MRENPWLAATGTVAPPLSRRGLRSWHPGKKTPGCKEQRRFANRA
                     SRRCGGSLLIACRVPHTRMSPCRRGLIQPQPAALTVQGK"
     gene            1263..1493
                     /locus_tag="ACHY09_004845"
     CDS             1263..1493
                     /locus_tag="ACHY09_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887686.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            1545..2906
                     /locus_tag="ACHY09_004846"
     CDS             1545..2906
                     /locus_tag="ACHY09_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFS3887687.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPCREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQRQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2953..3516
                     /locus_tag="ACHY09_004847"
     CDS             2953..3516
                     /locus_tag="ACHY09_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFS3887688.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVSEELLRGTFAYTDVPTMIIRLRA"
     gene            complement(3602..>3694)
                     /locus_tag="ACHY09_004848"
     CDS             complement(3602..>3694)
                     /locus_tag="ACHY09_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
                     /protein_id="HFS3887689.1"
                     /translation="NNNIISNRQMTGLKRQKSPSERPRNERGGA"
BASE COUNT          818 a         1025 c         1100 g          751 t
ORIGIN      
        1 atgcgtcagg cgctggacgg ggagaacggg taatgaatat cagcacggaa acccgcgaaa
       61 ttctgcgtaa ttacagggcc gtgattaatg cgcggcgtcg tgaaatgggg cagaaaccgc
      121 tcaccaccgc gcagattgtt gatgaaatct gcgattttgt ggcgaatcag caggcggttt
      181 tcctcggtgg tcactacatc cttcagggca gcaggcacag gtgatacagg gcaggcggtg
      241 gaggccgcct gtcagcccgc gcgaaaaccc ctggcaggct ggcgcctgcc ggaagggatg
      301 cgggccacgc tggcgcgtcg cccgttaccc ttcccgtttg tgatgtgtct gactcaggag
      361 tgtttctttc ttatgttatg gaactgttcc tgatgttgtt gttgttcttt tctctgctgc
      421 tctgattctg actactgcag tgttgtcctg tctgccgggc gcaccttcgg ctcccggctg
      481 cctggcggca tccggtcgtc aggcccgact ccgacaggca gctgctggcg cacccgccag
      541 tctccgccga acccgctggc gcggtttcgg gctgtcgcta cggtccgatg cgtgaaaacc
      601 cctggcttgc agcgaccgga acggtcgcgc cgcccttgtc gcgccgtggc ctgcgctcct
      661 ggcatcccgg caaaaagacg ccgggatgta aggagcaacg ccgtttcgca aaccgcgctt
      721 cacgtcgttg cggcgggtcc ctgctcattg cctgccgggt gccacacacc cgtatgagtc
      781 cctgtcgccg ggggctgata cagccacaac ctgccgcact gactgtccag ggtaaatgaa
      841 cagcccacaa aattttttac ccagaacccg gtaaaaaatt ttgcgttctg cccaggacag
      901 gtgcgtcagg ccgtggcagt gatgcccctt gcggcgaagg ttctcaaacg ggtgaagttc
      961 ataccacagg gaatacagca gcaggttccc gcgaaacgtc aaaaccgcgg ctcccgaaac
     1021 tcaacgggtg atgttcttta aaaatcagga tttatttcag gctccggcaa tacgcattta
     1081 tgtgttctgt cagcatcctg ctggcaggta atgagaacgc aggaacagaa ggaggaatac
     1141 accggaaaac gtcagatgca gatatgaaaa aagcaggtca gggacctgct taatccacgc
     1201 cgaagagact gtgcaaccag tctttaaggc actgttacca acctgtcata aggtatccat
     1261 ttatgactgt aagcattgtt tcaccttccg cagcggctgt aaagccccgc cgtcatcccc
     1321 gtttcagacg cgaagacatc cccgcgccgg aaattgaccc cgtgctgaaa gccttcgggc
     1381 gacacatagc ccggagtttt caccgggggc gtggtgtaca catccccgcc atgaaaaaca
     1441 cggctttcgg gcaggtgctc cgcacactcg aactgaaacg cgcttttaac tgatgaggtg
     1501 agccgccccg cgaggggcgg caacagacgg aaggagaaca ttttatgaca ttagcagcaa
     1561 tcaccatgac cgccccggaa gccgccagcc ctgtgcagat gtaccgcgcg acctactcac
     1621 cggatgacaa caaactgcgc ctgtatgccg cgtcacgtct tgacccggag acgtataaaa
     1681 aagtgcatga tgccggtttt cgctgggcac ccaaacaggc gctgtttgtc gcgccagcct
     1741 ggacaccgtg ccgggaagac gtgctcctct cacttgccgg agagattgag gatgaagaca
     1801 gcacgctcgc tgaacgtcag gaagcacggg cggagcggtt taccggatac agcggaaagc
     1861 gagccagtga atccgcacag gcacttgatg aagtggaaag actggccgcg atgatcccgc
     1921 ccggtcagcc cattcttgtg gggcatcaca gcgaacgccg cgcccgtcgt gatgcgcagc
     1981 gtattgaaaa cggcatgaaa cgtgctgtga tgctctttga acgtgcggaa tactgggaag
     2041 agcgggcgcg gtcggcactg cttcacgcga agtataaaga acgtccggac gttcgctggc
     2101 gtcgtatcaa aaaaatcgaa gctgatttgc gcaaggcgga aaagaccatc gcacagtcgc
     2161 agaaatatct gacgatgtgg cgggctgaat cgctggatct gaatatggca aaactcatca
     2221 gcagtcatga ccatatcagc gcctgtttcc cgctggatac gtatccgcgc ccggcagaaa
     2281 aaagccagta tgaaggaagt cgatcgttat ggtcggccct ggatgatgac atcatcacca
     2341 cggagcaggc ccgcgaaatt gcgatccgct gtcatgaacg gcagatccag cgtcagcaac
     2401 gctgggttaa ccactatcag aaccgcctga tctatgagcg tgccatgctg gacgaaagcg
     2461 gcggcgtggt tacccggaca caggattttg agccgggtgg acaggttttc agccggggcg
     2521 agtggctgac catcatccgc gtgaacaaaa gcaacggggc ggtgagttca gtcacaacgc
     2581 cgaattacag ttttctcggg tacagcggca cgatgaaagt gacgcccgat cgcattacgg
     2641 actacaaagc accatcggca gaagaggctg ccgtcgccag tcaggccgcg aagcgtccgc
     2701 cggtagtcaa ctatccgggg gaaggtttcc gggaaatgac aaaggcacag tgggccgccc
     2761 tgccccggga ctgtaaggcc gtgcgcagtg tggcagaagc agaagaccac ggggcatacc
     2821 gctaccgccg cacaatggac aataatttcc gcctggtgaa tgtgtatatc accgacatga
     2881 aaatcacgga aatcccgcag aaataaggta tatccccccg ggaaatcccc gggggatgac
     2941 ggagataatg acatgcacag tcagttaaga gaacgtatcc ggctgatgcg cgcaaggctg
     3001 gataacgccg cgccggttgc tgaaatccgg gctgaatctc agctttttgt gactcctgcc
     3061 ccggtctgtg atcgcctggt gacgctggcg gaaatcagca accgtgatca cattctggaa
     3121 ccctctgccg gcaccggggc catactgcgg gcgattcggg ataccgcgcc ggaggccatg
     3181 tgtgatgcgg tggagattaa cagcgggctg gtccggtatc tgcgggaaaa ttttaacggt
     3241 gtcagggttc agtgtggcga cttcatggaa tggcagccgg tgcagtatta cagccggatc
     3301 attatgaatc cgccgttcag tcacgggcag gatatccggc atatcctgcg ggctttttcc
     3361 ctgttgcgtc cggggggcgt tctggttgct gtctgtctca acgggccacg ccagcaggag
     3421 aagctgttac cgttttctga cgtcagcgag gagctgctgc gcggcacgtt tgcttatacc
     3481 gatgtcccga cgatgattat tcgtttgcgt gcctgatgac acagggctgg cgggaggata
     3541 aatgcccgtc tgtccgcgcc gggaaaaaac cgcgcgccgg tggcgctgaa aggggccgcg
     3601 ctcacgcgcc gcccctttca ttacgcggac gttctgatgg ggacttctgt cttttcagac
     3661 ctgtcatttg tctgttactg atgatgttgt tgtt
//
LOCUS       DAZUCC010000321         3689 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZUCC010000321 DAZUCC010000000
VERSION     DAZUCC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.323"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..1319
                     /gene="alaC"
                     /locus_tag="ACHY09_004849"
     CDS             81..1319
                     /gene="alaC"
                     /locus_tag="ACHY09_004849"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFS3887690.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYGIEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1384..1455)
                     /gene="ypdK"
                     /locus_tag="ACHY09_004850"
     CDS             complement(1384..1455)
                     /gene="ypdK"
                     /locus_tag="ACHY09_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFS3887691.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1811..2731)
                     /gene="lpxP"
                     /locus_tag="ACHY09_004851"
     CDS             complement(1811..2731)
                     /gene="lpxP"
                     /locus_tag="ACHY09_004851"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFS3887692.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3084..3326
                     /gene="yfdY"
                     /locus_tag="ACHY09_004852"
     CDS             3084..3326
                     /gene="yfdY"
                     /locus_tag="ACHY09_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFS3887693.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3403..3630)
                     /locus_tag="ACHY09_004853"
     CDS             complement(3403..3630)
                     /locus_tag="ACHY09_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887694.1"
                     /translation="MACNVFAFSISGGRSETSYKETEKTSVMTTTHSTKLQPSQAILF
                     KLREDAPPLNLTEDITPPFSTKANYLIHPVR"
BASE COUNT          930 a          872 c          942 g          945 t
ORIGIN      
        1 atcaggctat tttcaaaaca gccaccatcg tggcagcgtc gctcggacgg tccgggcgct
       61 aacgttaatc tgaggaaatt atggctgaca ctcgccctga acgtcgcttt acgcgcattg
      121 atcgtctccc gccctatgtt tttaacatta ccgctgaact gaaaatggct gcgcgtcggc
      181 gcggcgaaga tattattgat ttcagtatgg gtaacccgga cggtgcgact ccgccgcata
      241 tcgtcgaaaa attatgtacg gtggcccagc gcccggacac gcatggttac tccacgtcac
      301 gcggcattcc gcggttacgt cgcgccattt cccgctggta tcaggatcgc tacggcattg
      361 agatcgaccc ggaatcagaa gccatcgtca ctattggttc gaaagaggga ctggcgcatc
      421 tgatgctggc gacgctggat catggtgaca ctgtactggt gcctaatcca agctacccga
      481 ttcatattta cggcgcggtg attgccgggg cgcaggtacg ctcagtgccg ctggtggaag
      541 gtgtcgattt cttcaacgaa ctggaacgcg ccattcgcga aagttatccg aaaccgaaga
      601 tgatgatcct cggcttcccg tcgaatccaa ccgcgcaatg cgtggagctg gagttcttcg
      661 aaaaggttgt ggcgctggcg aaacgctacg atgtgctggt ggtccatgac ctggcctatg
      721 ccgatatcgt ctacgatggc tggaaagcgc cgtcaatcat gcaggtgcca ggtgcacgcg
      781 atgtggcggt cgagttcttt acgctgtcga aaagctacaa catggcgggc tggcgtatcg
      841 gctttatggt tggcaacaaa acgctggtca gcgcgctggc acgtattaaa agctatcacg
      901 attacggcac ctttacgccg ttgcaggtgg cagcgattgc ggcgctggag ggcgatcaac
      961 agtgcgtgcg cgacattgct gaacagtaca aacgccgccg tgatgtactg gtaaaagggc
     1021 tgcatgaagc gggctggatg gtcgaaatgc cgaaggcttc gatgtatgtc tgggcgaaaa
     1081 tcccggaacc gtatgctgcc atgggctcgc tggaatttgc caagaagctg ctgaacgaag
     1141 cgaaggtctg tgtctcgccg gggattggct ttggcgacta cggcgacacg catgttcgct
     1201 ttgcactgat tgaaaaccgc gaccgtattc gtcaggcgat tcgtggaatt aaagcgatgt
     1261 tccgtgccga cggtttacta cccgccagca gcaaacatat tcacgaaaac gcggaataaa
     1321 caccatcgcc agaaatgcaa acaggagcca gatggctcct gtttttcatt gcacagtttg
     1381 cttttagatc atcagggcaa aagtaccggc ccaaacgatg accataaaag aaatgcccat
     1441 aaagaaatat ttcactttac atctctccgc gtaattactg ttacgcctga atgacttcat
     1501 tacacctgat acagtgagtt gacaccgggc aaaacgacct taaaagtcct gtattttacc
     1561 gtgggcatca ccccagaatt ctgagctaaa ctgctccaga cggcgctaat gtacgcctaa
     1621 aactgaaacg agacaagagg cattttctta tttactttta gtaacttaca ggtgtctcat
     1681 atcggcgaca gtttaatttc tgacggtaat aattagagat tcagcgaagg aaaaaacctt
     1741 aagaaaaaag ccataaaaac catgaggtta ttatggctga tttgaggagg gaaagagtaa
     1801 gagcagtttg ttaaatgtat agcgacgatt ctcccaccgg gcgcgtttta aagcgacggt
     1861 ggatccagag gtactgctcc ggtgcgcgca tgatctcttt ttcgataatc ttgttcatat
     1921 aggcagcggc ttgattttca tctgccgggt agccttccat ctctggggtg atgtacaaac
     1981 gatatccgct gtaatctgct tttcttacca tcgttacggt taacatggct gcgccagaga
     2041 gacgtgaaag gacatacgta ccattggttg tggcgacatt ttccaccgca aagaacggcg
     2101 cgaaggagct gcctttacga ccataatcct gatcgggagc aaaccatacc gcttcacctt
     2161 tcttcagtgc accgacaatg ccgcgcagat tatttctgcc gatcatcgct ttgttagagc
     2221 gcatgcgccc acgggtctgc acccattcca tcagctgatt attatgtgga cgataggtag
     2281 ccatcattgg ttggcacagt cccatcacgc ggccgcccag ttccagcgac ataaaatgga
     2341 cgccgacaac cattacgccg cgattttgca tttgtgcgcg tttaaggtta tccaaccctt
     2401 caacatcaaa ccatttacgt acgcgactgt cgggccagaa ccacgccatg ccggtttcta
     2461 ccagcgccat gccgagtgaa cgaaagtttt cggcaatcat cttctcgcgt tcttccgcag
     2521 aatgctgtgg gaaacaaagt tccaggtttt tacgggcgat agattcacgc cgtttcagga
     2581 atggtcgcgc cattgcgcca atacgcgtgc cgagaaagca gagaacagga taaggaagct
     2641 gtacccaaag ccagagtaca ccaagcccaa accatgtgag ccagtagcga ggatgtagaa
     2701 actcgcggga aaatttgcat tgtggaaaca tagttacctt ctgtagatac gaattgttgt
     2761 atcgcagaag attccatgtc gacagcggta cactcaattg gttgggccat catgtacgac
     2821 aatggttcct gtaaagagtg taaagactag tcaggacata accacgagga gcatgaatgc
     2881 ggtggctcta ctttatcact ggctgactgc tttcagctac tgattattaa tcgtgcaaag
     2941 taaaaaattt tccgcacgca atagcgttgc atttaacgtg ctgttttcat tgatgcttag
     3001 caaaaattgc cagaggttaa ggattgtctt actattaaag tgactgaagt tacaggtttt
     3061 ttagcgcggc aggaggttga gctatgattt atttatggat gtttctcgcc ctgtgtattg
     3121 tctgcgtaag cggatacatc ggtcaggtac tgaacgtggt ctcggcggtc tcttctttct
     3181 tcggcatggt gatcctcgct gcactcattt actacttcac catgtggtta actggcggta
     3241 atgaactagt gacggggata tttatgtttc ttgccccggc ttgtggcttg atgattcgct
     3301 ttatggtggg gtatggcagg cgctagtggg tcaggatggt tcatttgggc aggtgttcac
     3361 atcagcatca ggcatgattg ttcacctgcc aggtgacaga cgctatcgca caggatgaat
     3421 aagataattc gcctttgtcg aaaaaggggg cgtaatgtct tctgtgagat ttaatggtgg
     3481 cgcatcttct ctcaacttaa acaaaatcgc ctgtgatggc tgaagttttg tagaatgagt
     3541 tgtcgtcatc actgacgttt tttctgtctc tttatagctc gtctcacttc taccaccaga
     3601 aatggaaaat gcaaaaacat tgcatgccat aatagagacc aataaaaata agctaaaaat
     3661 cattaagttt actttcatag aaagttatc
//
LOCUS       DAZUCC010000322         3685 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZUCC010000322 DAZUCC010000000
VERSION     DAZUCC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.324"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            70..1041
                     /gene="lpxM"
                     /locus_tag="ACHY09_004854"
                     /gene_synonym="msbB"
     CDS             70..1041
                     /gene="lpxM"
                     /locus_tag="ACHY09_004854"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFS3887695.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(1173..2615)
                     /gene="pyk"
                     /locus_tag="ACHY09_004855"
     CDS             complement(1173..2615)
                     /gene="pyk"
                     /locus_tag="ACHY09_004855"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS3887696.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(2743..3612)
                     /gene="hexR"
                     /locus_tag="ACHY09_004856"
                     /gene_synonym="yebK"
     CDS             complement(2743..3612)
                     /gene="hexR"
                     /locus_tag="ACHY09_004856"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFS3887697.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAIVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
BASE COUNT          904 a          918 c          943 g          920 t
ORIGIN      
        1 ggtgcggggc aagttgcgcc gctacactat caccagattg atttttgcct tatccgaaac
       61 tggaaaagca tggaaacgaa aaaaaataat agcgaataca ttcctgagtt tgataaatcc
      121 tttcgccacc cgcgctactg gggagcatgg ctgggcgtag ccgcgatggc gggtatcgct
      181 ttaacgccgc caaagttccg tgatcccatt ctggcacggc tgggacgttt tgccggacga
      241 ctgggaaaaa gctcacgccg tcgtgcgtta atcaatctgt cgctctgctt tccagaacgt
      301 agtgaagctg aacgcgaagc gattgtagat gagatgtttg ccaccgcgcc gcaagcgatg
      361 gcaatgatgg ctgagttggc aatacgcggg ccggagaaaa ttcagccgcg cgttgactgg
      421 caagggctgg agattatcga agagatgcgg cgtaataacg agaaagtgat ttttctggtg
      481 ccgcacggtt gggccgtcga tattcctgcc atgctgatgg cctcgcaagg gcagaaaatg
      541 gcagcgatgt tccataatca gggcaacccg gtttttgatt atgtctggaa cacggtgcgt
      601 cgtcgctttg gcggtcgtct gcatgcgaga aatgatggta ttaaaccatt catccagtcg
      661 gtacgtcagg gctactgggg atattattta cccgatcagg atcatggccc agagcacagc
      721 gaatttgttg atttctttgc cacctataaa gcgacgttgc ctgcgattgg tcgtttgatg
      781 aaagtgtgcc gtgcgcgcgt tgtaccgctg tttccgattt atgatggcaa aacgcatcgt
      841 ttgacgattc aggtgcgccc accgatggat gatctgttag aggcggatga tcatacgatt
      901 gcgcggcgga tgaatgaaga agtcgagatt tttgttggtc cgcgaccaga acaatacacc
      961 tggatattaa aattgctgaa aactcgcaaa ccgggcgaaa tccaaccgta taagcgcaaa
     1021 gatctttatc ccatcaaata aaaaatgcct ctcgcgagga gaggccttcg actggtgata
     1081 agttcaagtt tgcttcagaa gattcgaaat ctgttgaatt atcattgaac tgtaggccgg
     1141 atgaggcgtt ttcgccgcat ccggcaacgt acttactcta ccgttaaaat acgcgtggta
     1201 ttagtagaac ccacggtact catcacgtcg ccctgggtga caatcaccag gtcaccagac
     1261 atcaagtaac ctttatcgcg cagcagatta accgcttcgc tggcagccgc tacgccgtca
     1321 ttagcgctat caaagtgcac cggcgtaacg ccgcgataga gagcagtcag gttcagcgta
     1381 cgttcatggc gtgacatggc gaaaattggc agaccagagc tgatacggga ggtcatcagc
     1441 gcggtacgac ccgattcggt catggtgatg atcgccgtaa cgcctttcag gtggttagcc
     1501 gcgtacattg ctgacatggc aatggcttct tccacattgt cgaactgaac gtccagacgg
     1561 tgtttagaaa cgttgatgct cgggattttt tccgcgccca ggcaaacgcg tgccatggct
     1621 gcaacggttt ctgacggata ctgaccagca gcagtttctg cagaaagcat cacagcgtca
     1681 gtaccatcca gaacggcgtt tgctacgtcc atgacttctg cacgcgtcgg catcgggtta
     1741 gtaatcattg actccatcat ctgggtcgcc gtgattactg cgcggttaag ctgacgcgca
     1801 cgacggatca acgctttctg aatgccgacc agttccggat cgccaatttc cacaccgagg
     1861 tcgccgcgag caaccattac cacgtcagag gcgaggatga tgtcatccat tgcatcctgg
     1921 ctgcaaacgg cttccgcacg ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg
     1981 cgtgccagcc gacgggcata gttcagatct tcgccgcagc gtgggaagga gacagccagg
     2041 taatctacgc caattaacgc tgcagtctta atgtctgctt tgtctttttc ggtcagcgct
     2101 tcagccgaca aaccgccgcc aagtttgttg atacctttat tgttggagag gggaccaccg
     2161 acggtcactt cggtgaacac tttcatgccc tgaacttcca gtacttttaa ctgaacgcga
     2221 ccatcgtcca gcagcaggat gtcaccaggc acgacgtcag caggcaggcc tttgtagtcg
     2281 ataccgactt tttctttgtc gccttcacct ttacccaggt tggcgtcgag caggaattta
     2341 tccccaatat tgaggaaaac tttgccttct ttaaaggtgg atacacgaat tttgggcccc
     2401 tggaggtcac ccagaatagc cacatgacgc cccagttttg cggcaatttc acgaacttta
     2461 tccgcgcgca ttttgtgatc ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg
     2521 gcacccgctg cgataacttt ttcaaggtta ttatcgcgat ctgttgctgg gcctaacgtg
     2581 gtaacgattt ttgttctgcg aagccttctg gacatgtaat actccgttga ctgaaacaac
     2641 caggtgttgc ttgaacatga aatccgaatg aaataacgcc gcgatggcga taaattaatc
     2701 tcattcggat gtcgtataag attaggacag tgacagtcgt ttttagcgat cgtcacttaa
     2761 attaagtagc tgcttatcaa aacgcgattc cttcagcgct tccttgaccc gcttcaagtt
     2821 atctctgaat tttgcaccgc gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt
     2881 cagctgcgca agtcgagaaa ccatgggcat ataaatgtca gtatcttccg gtacgtcgag
     2941 ggtaattgcc agcgttgctt cccgggcgag cggggtaccc gcagaggtga gggcaatcac
     3001 tatggcgtcg ttttcgcgtg ccagctgcgc cagctcgacc agattttttg ttcttcccgt
     3061 gtgagaaatc agcaccacca cgtctccgtc gctacaattc atacaactca tgcgttgcag
     3121 cacgatatca tcggagtaca ccaccggaac attaaaacga aagaacttat tcatcgcatc
     3181 gtgggcaacg gcggctgaag agcctaatcc gaaaaaggcg attttttttg cctgagtgag
     3241 caaatcgacg gcgcggttga tggcagattt atccagtgaa tgacggacat gatcgagcgt
     3301 tgccattgcg gactcaaata ttttccccgt gtatgattca acgctgtcat cttcattgac
     3361 gttgcgatta acatagggag tgccattcgc cagactttgt gccagatgaa gtttaaaatc
     3421 aggaaaaccg cgagtgtcca tgctgcgaca gaaacgattc accgtcggtt cgctaacatt
     3481 ggcttccagc gccagagcag caatactcga atggatcgcg ttatcgggcg aagccagaat
     3541 gacctcggca actttgcgct cagatttgct caaatgttcc agctgagact ggattttttc
     3601 cagcatattc atgatgtaaa gagactcacg ggtaatgacg atttccgctc tgaaagaaat
     3661 cgaaatgcag ttttgtcaga tatta
//
LOCUS       DAZUCC010000323         3637 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZUCC010000323 DAZUCC010000000
VERSION     DAZUCC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.325"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(329..2371)
                     /locus_tag="ACHY09_004857"
     CDS             complement(329..2371)
                     /locus_tag="ACHY09_004857"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS3887698.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLTRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEISTFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSLLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGSLITAMKNASSGVT
                     EPALKKILRENAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALPDSLT
                     NPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLC
                     NGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSGDVGDKLN"
     gene            complement(2374..3078)
                     /locus_tag="ACHY09_004858"
     CDS             complement(2374..3078)
                     /locus_tag="ACHY09_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFS3887699.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
     gene            3124..>3637
                     /locus_tag="ACHY09_004859"
     CDS             3124..>3637
                     /locus_tag="ACHY09_004859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887700.1"
                     /translation="MRITDNKKTNQAEYTFNAAYFLIAVATGNTAHFLTSVATGSTVH
                     FSTSAATGSTVRFLTSAATGSTAHFLISAATGSTVRFLISAATGSTVRFLTSAATGST
                     VHFLTSAATGSTAHFLIPAATGRTVHFLTSSATGSTVHFLTSAATGSTAHFLIPAATG
                     STVHFLTSSAT"
BASE COUNT          839 a         1081 c          770 g          947 t
ORIGIN      
        1 gtagccaccc gaaggcgctc ctctcaggcc acttgtggag cattggtggt cgccggagcg
       61 acgttatgtg cccggacaga ctgaaatcac tgatggaaaa agtcaacagc taaccaaaaa
      121 caaaaaagca ccggcaagcc ggtgcttcag ggggatacca aatcccggtg ttgattctca
      181 tcttctccgg agagagcgac tcccggcacg gttttcacgg agtgcatgtg ccttgtaacc
      241 ccgccgtgtg cataacgggt taccggacct ctcagggctg tcagccccac tgtggtcaac
      301 agggtgatac cagaactcgc ccgcaatttt aattcagttt gtctccgaca tcaccagatt
      361 tttgtttatg agaaaaatta atttttcttt tcctgcccct gctgtttccc tgctcaccag
      421 caattcccct gcagtcggga taacgggagc atccccagaa aaaaccgctt tttcccttcc
      481 gtttcaccgt cggaccgtta cagagtggac atgaaggacc tgattccact gacagattaa
      541 ccgtctggtt acgtccggat aacaccaggt gcctgatcca ctgatgctgt ttctccataa
      601 acgcctccag tgagattttc ccccgggcaa catcatccag cgcctgttcc cataaagccg
      661 tcatacccgg atttgtcagg gagtccggca atgccgccac cagttctctt cccagttgcg
      721 tggaacggat atgttttccc gctctctcaa tatagttacg cgcaaataac gtttccagga
      781 tccccgcgcg tgtggcttcc gttcccagtc ccgcattctc cctcaggatc tttttcagcg
      841 ccggctccgt tacaccagac gaagcattct tcatcgcagt aataagactg ccttcagtaa
      901 aaggacgggg aggtacggtt ttcagctcct tctgttcagc accatccaga gcacagagtt
      961 ctccctttat caaagggggc aatatgtttc cactttcatc actgtcaggc ttttcttcat
     1021 ctgcatgatt ttccggtgga aataactgtt tccacccgat aatcacatta actcttccgc
     1081 gggaacggaa taactgcccc ccaatcacaa atgttgcctc agtaacatca gcttcctgct
     1141 ccggcagaaa ctgtgccaga tagtgctgac gaatcagttg atataccttc agttcatcac
     1201 cgttcagtaa gctgaggtca aaaacctgcc gggtcggaat aatggcatgg tgagccgtaa
     1261 tttttttatc attccagata cgggataccc gtcctgattc cagttcagcc agttgtggaa
     1321 gcacggacgg atctgaacgc ccgacagcct caagcacttc agaactctcc tcatgcatcg
     1381 aggcaggcag ataaccacag tctgtcctgg gataagttgt tgctttgtga gtctcataaa
     1441 gggactgtgc gatatccaga acggtctgag cccccatgcc aaacttccgg gaacaggctt
     1501 cctgcaacgt tcccagcgaa aaacacaaag gtggcggtgt tttctccctc ttctgtacaa
     1561 catcctgtac aacagcagag gactgctgac ggcacagacc gagaacagca ccggccgcct
     1621 gcacattcac acaacgtccc tcatcatcgc agtactgctc agctgccacc cagttactgc
     1681 ggaaacggag cccctccttt tccagccaga caatcacctg ccaccaggaa accggcacaa
     1741 atgtggagat ctcctgatcc cgtctgacca ccattgccag cgtgggtgtc tgcacccggc
     1801 ccactgacaa aaccccagaa attccacgtt cccgtgcctt cagggtatac aaccgggtca
     1861 gattcatccc tgtcagccag tcagcccggg cacgcccctt accggcttca ctgagaagcg
     1921 cagtctgttc gcccgggaga atattattca gcgccttcct gatactggcc tcatccagtg
     1981 cagataacca cagacgcctg acacgccctc tgtactgaca gtactccagc aactctctgg
     2041 ctatcacttc tccttcacga tcggcatctg tggcaataac cacttcagaa gcgcgtttta
     2101 acagcccccg gatcacaata agttgttttt tcaccttcgg cttcaccacc atctgccatt
     2161 gttccggtat cagcggcaac acatcagaac gccatgggga accgaactgt tcaccgtaag
     2221 cttccggctc agccagttcc agcaggtgtc ctatcgccca ggtgacagca atattgtcat
     2281 ctccgatgta tccgtcgccc cgcttactga gcgccagttc acgggcgata tctttcgcct
     2341 gagaaggttt ttcacacaga aagagttgca tactcacccc gctttaaccg gtggtgaata
     2401 aacagaacgt ttcttaccgc tcatcacgtc aggatccggc tcccccatgc ggttaaccgc
     2461 ctcctttcca cgggctgtgc cggacaaaat atcagcacgt gtaacactga ctgatttata
     2521 attttgaata atcccgtaaa tcctgcgtac cgcataccct ccctgattca gcaattcatc
     2581 ccgttttgtg cgggaaataa gtccgtaatg aaatacctga aataccttca tgaccagtcg
     2641 atcatatccg atcagaagcc atacacatcg ataaccgacc ggagaactac tgtacacgcc
     2701 aatatttacc ggcgaagagg atttaatatc agaaagggag atctgtcccg gcaggttttt
     2761 aaggatcccg tcaagccacg caagatgctt ttcaattgtt tcagttgcct tcgtcagggc
     2821 attttccagc ctgactaaca tcatatctgc ataaggatta tccctggctg aatctctggt
     2881 tgcctgaccc gcaaacgcaa tagccctggg catactgatg atgtcaggcc gcttttcctg
     2941 tttttcttcc ttctgattat tcctgcgtcc ctcccagaga cggatcgcat atcgggtatg
     3001 aagggcaata gtcagcgatg actgcagagc accaacttgt tctgattttc tgacttctcc
     3061 ggttttctcc tggggcattg tgtttactcc gaatgatttc atgaccagaa gatagtccgc
     3121 aggttgcgta ttaccgacaa caaaaaaaca aatcaggcag aatatacatt taacgccgct
     3181 tattttttaa tcgctgttgc cactggcaac accgctcact ttttaacctc tgttgccact
     3241 ggcagcactg ttcacttttc aacctctgct gccactggca gcactgttcg ctttttaacc
     3301 tctgctgcta ctggcagcac tgctcatttt ttaatctctg ctgccactgg cagcactgtt
     3361 cgttttttaa tctctgctgc cactggcagc actgttcgct ttttaacctc tgctgccact
     3421 ggcagcactg ttcacttttt gacctctgct gccactggca gcactgctca ttttttaatc
     3481 cctgctgcca ctggcagaac tgttcacttt ttaacctctt ctgccactgg cagcactgtt
     3541 cactttttga cctctgctgc cactggcagc actgctcatt ttttaatccc tgctgccact
     3601 ggcagcactg ttcacttttt aacctcttct gccactg
//
LOCUS       DAZUCC010000324         3632 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZUCC010000324 DAZUCC010000000
VERSION     DAZUCC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.326"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..252
                     /locus_tag="ACHY09_004860"
     CDS             <1..252
                     /locus_tag="ACHY09_004860"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS3887701.1"
                     /translation="TYIQKASVSSSVTSLRRPMFLKFPIPVPPSEEQARIVEILDKFD
                     TLTNSITEGLPREIELRQKQYEYYRDLLFSFPKPETVSN"
     gene            372..3533
                     /locus_tag="ACHY09_004861"
     CDS             372..3533
                     /locus_tag="ACHY09_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS3887702.1"
                     /translation="MILQTHTIAETSNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     QNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWRRFTEQYLDNPGDGILDKTRK
                     IHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQFEQAGSHSNRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQLHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFRRYYQFGFTGTPIFPENASGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSRGFNAMLAVSSVDAAKVYYATFKRLQEEAAN
                     KSANYKPLRIATIFSFAANEEQNAIGEISDETFDTGAMDSSAKEFLDAAIREYNDHFQ
                     MNFSTDSKDFQNYYRDLAQRVKNQDVDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRNLEQATIDAITLFGDKNTRNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVSELEQRFPDPASIESEKEKKDFVKLFGEYLRAENVLQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQMIRLPAERKIQDYRSAYNDIRDW
                     QHREKEADKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHSRQNKSKDEMTVEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDTFFTFAQREQQREADALIKEE
                     NLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKISAFIEK
                     FKGVGGASLVTGAQTPPCLNKQA"
BASE COUNT         1117 a          829 c          799 g          887 t
ORIGIN      
        1 acctatattc aaaaagcatc agtaagttca tcggttacct cacttcgtcg accaatgttt
       61 ttaaaattcc caatacctgt accaccttca gaagaacaag ctcgcattgt cgaaatactc
      121 gataaattcg acaccctgac caactccatc accgaaggtc tcccgcgtga aatcgagttg
      181 cgccagaaac aatacgagta ctatcgtgat ttactgttca gtttcccgaa acctgaaacc
      241 gtcagtaact aattgacaat tgctaccgac cgggccacct taacatccgg tcagtatata
      301 aaccattttt acacgccgga agtcaccctt aactcccttc cggccccgcc agacggcaca
      361 aaggatgcgc tatgattctt cagacacaca ccattgctga aaccagtaac tttatcgttc
      421 ttgataagta catcaaggct gagcaaacag gcgacagcta ccaaagtgaa tcggacctgg
      481 aacgtgaact gattcaggac ctgcagaatc agggctatga atttatttcc gtaaaatcac
      541 agtcggcgat gctggctaat gtccgggaac agcttcagag cctcaatggc gtggtgttta
      601 atgacagcga atggcggcgt ttcacggagc agtatctgga caaccctggc gatggcattc
      661 tggataagac ccgtaaaatc catatcgact atatctgtga ctttattttt gacaacgggc
      721 gtcttgagaa tatctatctg atagataaaa agaatctcat gcgcaacaaa gtgcagatta
      781 tccggcagtt tgaacaggca ggttctcaca gtaaccgtta tgacgtcact atcctggtta
      841 atggtttgcc gctggtgcaa atcgaactga aaaagcgtgg cgtggcgatt cgtgaggctt
      901 tcaaccagtt acatcgttac agcaaggaga gttttaacag cgaaaattcc ctgtttaagt
      961 atctgcaact gttcgtcatt tccaacggta ccgatacccg ttactttgcc aacacgacaa
     1021 agcgcgataa aaacagtttt gacttcacca tgaactgggc gaaatcagac aacacgctga
     1081 ttaaagacct caaagatttt accgccacct ttttccagaa acataccctg cttaatgttc
     1141 tggtgaacta cagcgttttt gacagcagtc agacgctact ggtgatgcga ccgtaccaga
     1201 ttgctgccac cgagcgcatt ctgtggaaaa ttaagagctc ctttacagcg aagaactggt
     1261 caaaaccgga aagcggtggg tatatctggc acaccacggg ttccggtaaa acactgacca
     1321 gctttaaagc cgcgcgtctg gcaacagaac tggactttat tgataaagtc ttctttgtgg
     1381 tcgacaggaa agacctcgat taccagacca tgaaggaata tcagcgtttt tcgccagata
     1441 gtgttaacgg ctcggaaaat accgcaggcc ttaaacgaaa tctggataag gacgataaca
     1501 aaattatcgt caccactatt cagaaactta ataacctgat gaaagcagaa agcgacctgc
     1561 ctgtatacaa tcagcaagtg gtgtttatat ttgatgaatg ccaccgcagc cagtttggag
     1621 aagcgcagaa aaatctgaag aagaaattca gacgctatta tcagtttggt tttaccggca
     1681 cacctatttt cccggaaaac gcctcaggtt cagaaacgac cgccagcgta tttggtcgtg
     1741 aattgcattc gtatgtgatt accgatgcga ttcgtgacga aaaagtgctc aaattcaagg
     1801 tggactacaa cgatgtgcgg ccacagttta agtctttaga gacagaaact gacgagaaaa
     1861 aactgagtgc ggctgaaaat cagcaggcgt ttcttcatcc catgcgtatt caggaaatca
     1921 cgcaatatat cctgaataac ttccgccaga agacccaccg cactttcccg ggttccagag
     1981 gttttaatgc catgctggca gtaagcagcg tggatgctgc gaaagtctat tacgcgacgt
     2041 ttaaacgctt acaggaggaa gccgctaata aatctgcaaa ttataaacca ctgcgtattg
     2101 cgacaatatt ctcctttgcc gccaatgaag aacaaaatgc cattggtgaa atttccgatg
     2161 aaacttttga taccggcgca atggacagca gtgctaaaga gtttctggat gctgcaattc
     2221 gtgaatataa cgaccatttt caaatgaatt tcagtactga tagcaaggat tttcagaact
     2281 actatcggga cctggctcag cgggttaaaa atcaggatgt tgacctgtta atcgtcgtgg
     2341 ggatgttttt aaccggcttc gatgctccaa cactgaacac gctgttcgtc gataaaaacc
     2401 tgcgttttca cggtctgatg caggcgtttt cccgtaccaa ccgcatttat gacgccacga
     2461 aaaccttcgg taacatcgtc acattccgta acctggagca ggcaaccatc gatgccatca
     2521 cactttttgg tgataaaaac accagaaatg tggtgctgga aaagagttat gcagagtata
     2581 tggaaggctt tacagatgct gccactggcg aagctaaacg cggctttatg gcagtagttt
     2641 cagaactgga acaaaggttc cctgaccctg ccagtattga aagcgaaaaa gagaaaaaag
     2701 acttcgttaa actgtttggt gaatacctgc gtgccgagaa cgtcctgcaa aactatgatg
     2761 aatttgccac gctgaaagcc ctgcaacaaa tcgatctcag cgatcctgtt gcggtagaaa
     2821 aattcaaagc agaacattat gtggatgatg aaaagttcgc tgaattgcaa atgattcgtc
     2881 tccctgctga acgcaagatt caggattatc gttctgccta taacgatatt cgtgactggc
     2941 agcaccgcga aaaagaagct gacaaaaaag aaaaatcaac cactgactgg gatgacgtgg
     3001 tttttgaggt cgatttgctg aagtctcagg aaataaacct ggattatatc cttggcctga
     3061 ttttcgaaca cagcagacaa aacaaaagta aggacgaaat gaccgtagag gtcaaacgat
     3121 taattcgttc aagtctgggg aaccgtgcta aagaaggcct ggtggtcgat tttattcagc
     3181 aaacgaacct tgatgatttg ccggacaaag ccagcatcat tgatacgttc tttacctttg
     3241 ctcaacgcga acaacaacgt gaagcagatg cattgatcaa agaagaaaat cttaatgaag
     3301 aagcggccag gcgctatatt cgcacgtcgt taaaacgcga atacgccacc gaaaatggca
     3361 cagaattaaa cgagacatta ccaaaactta gtccgttaaa cccgcaatat aaaacgaaaa
     3421 aacagaccgt ttttcagaag attagcgcat ttattgagaa gtttaaaggc gtgggggggg
     3481 catctttagt tacaggcgcc cagactcccc cttgcctcaa taaacaggca tgaacactga
     3541 ctgctattgt aaggcgttaa cggagtccgt attgacgctt acgctcaagt cacgactaat
     3601 acaactaaat gaatgattaa cagctcagct aa
//
LOCUS       DAZUCC010000325         3598 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZUCC010000325 DAZUCC010000000
VERSION     DAZUCC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.327"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(107..1426)
                     /locus_tag="ACHY09_004862"
     CDS             complement(107..1426)
                     /locus_tag="ACHY09_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFS3887703.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPTGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMVDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1557..3092)
                     /gene="gadC"
                     /locus_tag="ACHY09_004863"
     CDS             complement(1557..3092)
                     /gene="gadC"
                     /locus_tag="ACHY09_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFS3887704.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3248..>3598)
                     /locus_tag="ACHY09_004864"
     CDS             complement(3248..>3598)
                     /locus_tag="ACHY09_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFS3887705.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
BASE COUNT          939 a          890 c          902 g          867 t
ORIGIN      
        1 tgcgtagtta atttgactta agtaagcatg attactagtg ggatatttca agagggtaac
       61 aagccggggg gaaagcaccg gcttgttaca aagtaagaat gggagtttaa ctgccccaac
      121 gactttgcag atagctgacc gcttgttgag tctgtggctt attcagatag tcctcacgga
      181 acaagattgt gccgctaatt tctggcacgg catcgttaag atcgagctgc tttttcagtt
      241 ccggtacgcc gccgttaatc atccagtctg gctctatctt tgaaggttca cccactttat
      301 agaaggcgat accgatatac aggcgggtcc tggtcggttt aacgacatcc gcccaccatt
      361 ttgccaaaac gtcataacgt gcggcactcc gtgagaacgg ccagtaaatt tggggagcaa
      421 tgtaatccag caatccttgt tccacccatc gacgggtgtc agcgtaggat tcgtcatagg
      481 ctgccgcgcc tcgggtatcg gaaccgagcg gatcgtgtga tcggttacgc cacacgcctg
      541 ccgggctaac gccaaattcg acttccggct taatgctttt aatagtgtgc gatacctttg
      601 caattaactg ctgagtattg ttgcgccgcc agtctgcttt tgacgcaaat gcgcctccgt
      661 atttgcggta cgtttcgtta tcatttagcc gtgaacccgg tgactcagta tagaaatagt
      721 cgtcaaactg cacgccatct accggatagc gggaaaccac ttccgcgact attgatgtga
      781 tccagtcctg aacttcaggg atgcccgggt cgaggacaaa gcgatcgccg gacgttctga
      841 tccagtcgcg gtgttgcaca tagacgctcg ctggttgttg agacagagtg ctattcagtt
      901 ccctgatagt accgggcttc gtattaaccg atacgcgata ggggttaaac caggcgtgta
      961 ctttcatccc acgcttgtgg gcttcgtcga gcataaattg cagcggatcg taacctggat
     1021 tttcaccaat ctttccggtc ataagatcgg accacggcaa aatttttgat ggccacaggg
     1081 cggtaccgtc cggcttgacc tggaaaaaga ccgtgttgat gccgagacgt tgcagatgat
     1141 ccagtttgtc gaccatcgcc tgttgttgta cacgggcccg gctggtgggg ttactaatgt
     1201 taaccgagga aaccggtggc cagtcgaggc gagacactgt ggccagccag atgccacgca
     1261 tcggttgtga cgattgttgc gtcgtagctg gtggcttcga acctgttggt ggtgtcacca
     1321 ttgactctgg aggcgtgctt ttgcaactac acagtaaaag tgccagtgca accagtatcg
     1381 ctggtcttct aatcgctaat ttctcgtttc gggagcagat atccatatgt gctggtttcc
     1441 ggtaaacaga tgtgcgctca ttctcgtact tatcccggtt aagtcaatac gacaacaagc
     1501 acgaaaaagg gagcgatgaa ttatcgctcc cttgtcttat aaccattcag acatggttag
     1561 tgtttcttgt cattcatcac aatatagtgt ggtgaacgtg cacgcgggtg caggaagaag
     1621 tgaccttttg gtgcgttctg actgttgatt ggctccagag tgacgccggt attcgctttg
     1681 cttttacgat catgaacagc atagagaata aagggcaggg caagtaccac caggaaactc
     1741 acgaccagta attcaacata catatcggta gagtcaccct ggatgttatc cggcggcagg
     1801 aaggaaacaa taaacgccat aattgaagtc agcagaccga caatagccac gaccagtttc
     1861 acccctttac caccagggat attaaatgtg cgttttaagt caggatgttt aagaaccaac
     1921 acaatgtagc caataaacag catgaaataa gcacacagat aaatcaccac cgtcagcgcc
     1981 agtgcgatca ggaaggacat gttgttaccg ccaccggtat tggtgaggat gatcaacgcg
     2041 atagaagtaa tcaccaactg cgaaatgacc agcgttaccg gtacgccatt tttgttcatt
     2101 ttagcgaatg ccgctggcag caggtttttc tgcgctgtga catacatccc gcgagaagga
     2161 ccaacaatcc aggaagcgat ttccgccaga acacccagca acagcagtgc ggagatcacg
     2221 cgaaccgtcc actcaatttc cggtgccaca tgtgacatca gaacggtaaa ggtttgcatt
     2281 acccctgcgg agaggttgat ttcattaccc ggaatgacca tcgcaataga caaaccgcca
     2341 acagagctta agcagattgc cgccaccatc agcagtaaca tagccagtgg atagtcgcgc
     2401 ccgggattgc tcatttcatt gacgtgggtt gcggaagctt caacgcccat ataactcaaa
     2461 atgaaggcaa caaatacaac cagggtgccc actttagaga agtcagggaa gaaggtcttc
     2521 gaatccattt cgatagcaac gggggcaccg gagtgcagat aaatagccgc taatgcgatc
     2581 aaaataaatg caggtaacag gataccggcg aagaagccaa ctttagcaat tcgcgccgtg
     2641 tatttcgtgc caccaaactg cgttaatgcc agcgcccaaa gaatgatgag tgctgcaata
     2701 gttttggtaa tggggtcttc attcagcgct ggccatttca ggatgtagga gagtgcccct
     2761 aacacgaaat agagcatcgg aataaaacca atggcgattt gcagatagcc aaatgagatc
     2821 gctgcaaatc cccatctcgg ccccagagta tttgataccc aggcgaagac gccaccttct
     2881 tcccagccgt cgacggtagc catttccgca gcacaaagtc ccacgggaat aaaccataaa
     2941 atcccgccta atagcaggaa gaagactaat gaaaagcccg atgttgcgaa ggtagggtat
     3001 tcataaacag ccattaccat cgatgccgtt atggcaaaaa atccgagtaa tgtgagctgc
     3061 ttagctttac ctgtctgtac tgatgtagcc atattatccc cctaaaacgg tattcctgtc
     3121 ggaaccgcac ctgttttgtt ctgaagcgta ttcagaacaa tattttccgt tgctaatgcc
     3181 agtgaacaga ctttggaaat tgtcccgaaa cggcttcgtt tcgggacacc gttaccgtta
     3241 aacgttatca ggtgtgttta aagctgttct gctgggcaat accctgcagt ttcgggtggt
     3301 cgctgagata tttcagggag gctttgtagt cttccagcaa cagttcagca aagtccattt
     3361 cgaagccgcg acggcacata atgcgcatca ccacgatgtc ggtggcttca ccgcctagag
     3421 tgaaagccgg aacctgccag ccgcgcagac gcaaacgttc agagaggtca tacagggtgt
     3481 atcccggatc ttcaccatct ttcagtttga agcaaaccgc cgggatgcct tcgtccgggc
     3541 gacccgtaca gatgaactca tacggcccca gtttggcgat ttcatccgcc agataagc
//
LOCUS       DAZUCC010000326         3565 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZUCC010000326 DAZUCC010000000
VERSION     DAZUCC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.328"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..296)
                     /locus_tag="ACHY09_004865"
     CDS             complement(<1..296)
                     /locus_tag="ACHY09_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFS3887706.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILT"
     gene            complement(296..1663)
                     /gene="dnaB-PI"
                     /locus_tag="ACHY09_004866"
     CDS             complement(296..1663)
                     /gene="dnaB-PI"
                     /locus_tag="ACHY09_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFS3887707.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESLLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVDYLQLIRA
                     PELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDG
                     PGGYA"
     gene            complement(2011..2913)
                     /locus_tag="ACHY09_004867"
     CDS             complement(2011..2913)
                     /locus_tag="ACHY09_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887708.1"
                     /translation="MSSSVKYSPVPAVLTRPESLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQRVPGDSVNCMCWASLAALAFDAEERVELQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCTGRIQAGRHHSGRHPDVSVYAERCAQ
                     ETSLSPVHPARQCPVQSTDSAETE"
     gene            complement(3149..3358)
                     /locus_tag="ACHY09_004868"
     CDS             complement(3149..3358)
                     /locus_tag="ACHY09_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887709.1"
                     /translation="MCVGCGYSGVRDGAGKNAPVWVKVNPESGLGLCSGCDAFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
BASE COUNT          853 a         1056 c          901 g          755 t
ORIGIN      
        1 tccgtcagta tctggtaacg ggtgtttccc ccgtcactga agatatacac atccggctca
       61 ctgtccggca tacgggtgac tttgggcacc gtatccagcc cgcgggcatg aatggaattt
      121 ttgatatcgt cataacgcgg attgcgcgtg gtgcgcgggt tatccggatt cggacgtaac
      181 tggtccagcg tcagaaccat gggcgtttcc cccattgcca ccacattgcc ggcaccagtg
      241 gtctgacgac cgggctgcat aatagccgcc ccgagatcca gtggtttatg tgccatcacg
      301 catatcctcc cggcccgtca ttctcagagg gcgtaaacag tgtgtaacga ccgtcgaacc
      361 tgacccgtac cgtacccgtg ggcccctgac gctgcttgct gacaatgatt tctgcctccc
      421 cctttgccag tgagtcgggg ttatagacct cgtcgcggta aataaacata atcaggtcag
      481 catcctgctc cagcgccccg gaatcacgca ggtcaccgtt attggggcgt ttatcagcac
      541 gctgctccac ctgccggtta agctgggaga gggccagcac cgggcagccc agctccttcc
      601 ccagcgcttt cagggaacgg gaaatttcag caatttcctg tgtgcggttt tccagctccg
      661 gcgcacgaat cagttgcaga taatccacca taatcaggga gggtttaccg tacagacgtg
      721 tataacgacg ggcgcctgca cgcagtgatg aaggcgtctg ctcacagcag tcatcaatta
      781 ttatgcgctc acccagagag cccattcctc cgtcaccaag cgccataccg accgcagatg
      841 acaccctcag ccagtcctca tcatccatgt tgccactgcg caaatgattc agttccacac
      901 gccccagcat ggaaagcatg cgcagaatca gttgctcctt cggcatctca atgctgaata
      961 ccagaacgct ctcttccgtt cgccgcagtg cggcgctgag acacatggat agcgccagag
     1021 aggttttccc cattgacgga cgggcagcca gcagaaccag gtcaccaggc tgcaggccac
     1081 aggtcatttc atccagacag tcaaatcccg tgggggtacc ggtaacacca ccggagccga
     1141 gcaaactttc cagttgctgc agaacggtat caagcgcgga tgaaagagag gcttctgtgc
     1201 gttcccggag cgtcctgttc tgcgcaagtg caaacagacg ccgctctgca gcatccagca
     1261 catcccgtga tgtggcgttc ggtgccgcca catcagcaat cagggtgttt ccggtggcct
     1321 gtaactgacg cagaacgctt ttctccacca caatgcctgc ataggccatg atgttggccg
     1381 cagaaggtgt gttcttgctg agctcggcca gataagcaaa accgcccagt acgtccagcc
     1441 tgccctgatg ttccaggtgc tctgacagtg taatcaggtc cagtggctgg ccattcaccg
     1501 ccagttcaga catggcacgg aaaatggccc ggtgctgagc cacataaaaa tcgtcaggac
     1561 tcagcatcag caccacttca tcccagcgtt cattctccag catcagccca cccagaacgg
     1621 cctgctctgc ttcgtatgcg cagagaacag acatttcagc catggcgtac cctccctctc
     1681 tgactgccgg aaacctggct acgtgaaaaa aggaaaaagg gtgccggaca tacagctgtc
     1741 gggaataaaa tcccggtaac tgtctgtacg ttttcagacc ggtgagtttg ccgaccccgg
     1801 tctgaaggta cggacagcat tcgttcattc ataacaagtt gttttaaagg aaaaagttta
     1861 tgaatacaga aaaacaactg ccgtccggca ttgaatctga agtgaaatac accctgcagt
     1921 tctggctgct gagtaacgga aaggtcatga cggaaaaaga cgggctctgt aacccctttc
     1981 ccccgaactg tattgaatcg gctcatcctt ttactccgtt tcagcagaat cagtggattg
     2041 aactggacac tgtcgggccg gatggaccgg tgaaagcgac gtttcttgtg cacagcgttc
     2101 tgcataaact gaaacgtcag ggtgtctccc tgaatgatgc cggccggcct gaatacggcc
     2161 ggtgcattgc tcactggcac actgacgtgt ttgttgagaa aaaagcgctg cctgactggc
     2221 tgagagcatt ccacactgac gtttcgcact cgtaaaaaca cgttcagccc gacgggtcat
     2281 attttccagc cacccgtcgg ggaaaatgtc agcaccatca cggggaaaca aaatcgcttt
     2341 ccattcaggg tatgagcgcg gaatacctcc ggaagcattc cggctcagac agcgactcac
     2401 agtgcaggca gcacggaatg catatgttgc gatgctctca tcatatgttg cgatgttctc
     2461 attattgcct ttctgcagtt ccacccgctc ttcagcatca aatgccagtg cagcaaggct
     2521 tgcccagcac atgcagttca ctgaatcccc cggaacgcgc tgcagctccc ggcgcaccat
     2581 gttgacctca gcactgaaaa aagcgttctc atcgccttca agggcagcca gatgcagcgg
     2641 aagccagacc tgactgaacc attcgcacat ccgttcccgg ttactgacgg tcatcactgt
     2701 ctccgggcgg gaaccgtcct gaaaaacatg tccggtgcgt ggagaactgt atgaaacata
     2761 accaccgtgc accgcaatga ccggacgacc tgacgccgga agccccacac gacacccact
     2821 ggcaatccgg gtcatcagca ggttcagttc agccagcgat tcagggcggg ttaagaccgc
     2881 cggaaccgga gaatatttca ctgaggaaga catactttca cctccatatc tctcagcccg
     2941 ccaggtgcgt cacttccccg cagataaccg gaaacctggc tatatgaaaa aagggaaaag
     3001 ggtgccggac gaacagctgt cgggaataaa atcccggtaa ctgtctgtac gttttcagac
     3061 cggtaagttt ggcgaccctg gtcagaaggt acggacagca tctttgcgct cataactgta
     3121 ccgaccggga tatgcattac aaagactgtc ataccgcccc ctccgtcttt cgctgtttgc
     3181 aggaaagacc acatgaaaaa agctggtcat gcctctgacg acgctggcgt tcctgcaaaa
     3241 gaaaggcgtc acaaccggaa cacagcccca gaccactctc aggattcacc ttcacccaga
     3301 ccggagcatt cttaccggca ccatcccgta ccccggaata accacagccc acgcacaccg
     3361 tccacttctt cagtacgatg agtgcagttt cgagtgatgc gcggagacgc cgggaggcaa
     3421 taaggagcaa cggctcatgg tcataaaaaa ccccgccgtt accccctccg gattcaagca
     3481 gcgggagtcc tgcaggctcc cggggacctg ttgacatttc ccgtaattcg ttttcattcc
     3541 acatattgtg gaaagttatc cggtt
//
LOCUS       DAZUCC010000327         3542 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZUCC010000327 DAZUCC010000000
VERSION     DAZUCC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.329"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..575)
                     /gene="ogt"
                     /locus_tag="ACHY09_004869"
     CDS             complement(60..575)
                     /gene="ogt"
                     /locus_tag="ACHY09_004869"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS3887710.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISAANPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(586..729)
                     /locus_tag="ACHY09_004870"
                     /pseudo
     CDS             complement(586..729)
                     /locus_tag="ACHY09_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            729..945
                     /locus_tag="ACHY09_004871"
                     /pseudo
     CDS             729..945
                     /locus_tag="ACHY09_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1119..1934)
                     /locus_tag="ACHY09_004872"
     CDS             complement(1119..1934)
                     /locus_tag="ACHY09_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3887711.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            2031..3059
                     /locus_tag="ACHY09_004873"
     CDS             2031..3059
                     /locus_tag="ACHY09_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS3887712.1"
                     /translation="MIISKKQLIGVVAIGIILAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSRKANLES
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLVQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            3056..>3542
                     /locus_tag="ACHY09_004874"
     CDS             3056..>3542
                     /locus_tag="ACHY09_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3887713.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGSAAAVVSALASLSPTLGPTLGGWITDNLD
                     WR"
BASE COUNT          947 a          786 c          860 g          949 t
ORIGIN      
        1 agttgttaag ggtaacttta cataaaagtg tgaacaagct ggcacaaatt gtttaatgtt
       61 tacagcaaaa gataaccttc gtggcgcaat aaccactctt ttcgctgaac tccgcctgca
      121 tatccggtca tggtgccgtt tcggccaata acccgatggc aaggtacaac aatgctgatg
      181 ggattcgatc cgttagccgc accaacggca cgcgccgcgc cagggcgacc caattgttcg
      241 gccagttgac cgtaatgcat tacctgcccg caggggatag tgcgtagtgt tttccagact
      301 tcgcgctgaa atggcgtccc ccccgtggca gtgggaagcg tatcaataat gctaagatta
      361 ccggcaaaat attcacgaag cttgtcgctt aaaccacctg ggttggcggc agaaatgcgc
      421 tcatagcctt ctttgcgata atggatgtcc agcagctgca ccatgcgttc gctgtactct
      481 tcccattcaa ccgcccgcag gcgaaattgc tcatcgcaaa tcacccatag tggacccagt
      541 ggcgtggcaa ttttttcttc aagtaatctc agcatccgtt ctctcttaag acaaacgtgg
      601 gtaaatcccc ggacctatcg gcagaccaac aagataccac gccagcaaca tcagcagcca
      661 taccaccaaa aagataagcg gatagggcaa gactaacgaa tagtaagtac ccagtttcgc
      721 gtctggtttg tcgcagtctg atagcataaa gcgatttttt gatttttctg cctgaagaaa
      781 tgagggcaag ccatcacatg gatcgagggt gggataattc cagtcagcga aattttaccg
      841 taggctatct gttatttgat tctgtggcgt gatagccgcc agacaccact gatctcgtca
      901 ctcattcccc aatcccggcg agaatgtggt tatttacaga gctaaaaaat gaataaaaaa
      961 atagagcctg tcgacatccg ccagactcta cagtacacac agcagtgcat cctcgtctta
     1021 aatccggcct ggggtcctgg tgaccggtga cagtgttata acggttttac ccatccgttg
     1081 ccaattttct tcagtgacag atttcacgaa aatataagtt aaatcatttg ccttgtgatt
     1141 gcataatcat gcatcgttaa tcgacgagta aatttaccgg gcgagtagcc cgttcgacgc
     1201 ttaaacatat tgccgaacga ttcagctgag gcatagccca gacgatgggc aattttggtc
     1261 aacgaatatc catcatcaag aagcgtcact gcagaaataa cgtgcatttg ctgaacccag
     1321 tggcgaaatg ttaatcccgt ctctttaaca aataagcgca tgagtgtacg gctactgacg
     1381 ttaattttat ccgctagatt atccagtgtt ggcagatacc cttggttgtt tagcaaattc
     1441 tcacaaagct gctgtagccg ctcatcattt ggccaaggga gttcaagtga cattttcacc
     1501 cccattttta tttcctgaag tatcagtgtc tcgagggctg aaaaacgtaa ctgttgttta
     1561 tctgtggtac tttgcgacgg tggaagttgg ttaattgcaa ttagtaattc tcttaacagg
     1621 ggtgaaatac ccgtcatgaa gcaaactttt cccagcgttt cactttccgc ttctgaaacc
     1681 agtacggttt ttaaaagtac gttgcctgtc gcaacaaact tatgcggcat ttgtgggggc
     1741 agccaaagcg cgctgctggc atgcactagc cagacgcttt ctccagccca tacacgaacg
     1801 actccttcag ccgggtaaag caactgggca tagagatgtt tatgccaggg aactgtgcct
     1861 ccattcagat gttccgaatc cagtccgata atgtgatgcc ctggtgatgc tttgggttgc
     1921 agtgtgattg tcatttacag taggtatttt gtcagaaatc atcaaataag ccatgttaaa
     1981 ttttaaatat ggcgaatgca agtttccgga tatatacggt gtaaagagtg atgataattt
     2041 ctaaaaaaca gcttattggg gtagtagcga tcggtattat tttggctggc gttgtctttt
     2101 ttatctggtg ggtaagcaaa ggaagattta tacagacgac ggatgatgct tacattggtg
     2161 gtaatattac cacggtggca agtaaagttt ctggttatat ttctgctata gaggtgcgtg
     2221 ataatcagag cgtaaaaaaa ggcgatatta tcctgcgtct cgatgatcgg gattatcgtg
     2281 caaatgtcgc caggcttgag gcaaaaataa aaagtagaaa agccaatctc gaaagcattc
     2341 aagccactat tacgatgcag cagtcgataa ttcaaagtgc cagtgaaaca tggcaggcag
     2401 taaaacatga ggagcaaaag cgcctgcgag atactgaacg ttatgaaaaa ctcgtacaat
     2461 cagcggcaat atcgcagcaa attattgata acgccaggtt tgactaccag caggtcgcag
     2521 caaaggagcg caaagccgcg aacgatttcc tggtggagaa gcaacggctg gcagttttat
     2581 ctgctcagga agaaaacgta cgtgcgagta tcgaagaagt tcaggccgct ttgactcagg
     2641 cactactgga tctggaatat acccttgttc gtgcgccgat tgatggcatt gtcgcaaacc
     2701 gcagcgccca tacaggcagt tgggtggaag gcggcacatc gttagtttca cttgtaccag
     2761 tcagtgagtt gtgggttgat gcaaattata aagaaaacca aatagcggga atgaagccag
     2821 gaatgaaggc tgagatccgt gctgatattc tcaaagggga agtgtttcac ggtcacattg
     2881 aaagcctttc cccagcgaca ggagccagtt tcagcttgat tccgatagaa aatgccactg
     2941 gcaatttcac caaaattgtt cagcgtgtcc cggtacggat tgcatttgac gacgcaaagg
     3001 aattaaagca acttcttcgc ccgggactgt ccgtcaccgt cagtgtcgat gagagatgat
     3061 gtctatgcgt aaacatattg ctttcgccag tatgtgtatg ggattattta ttgctcagct
     3121 tgatattcaa attgtatcgt catccctaaa tgaaataggt ggtggtctgt ccgcaggtaa
     3181 agacgaaatg gcctggcttc agaccagcta tttaattgct gagattattg ttattccgct
     3241 ctctgggtgg ctgtcacggg tattttccac tcgttggctt tttactctat ccgcgggaat
     3301 atttacttta atgagtatcg cgtgtggact tgcatggaat attcagatca tgattttgtt
     3361 tcgtgcttta cagggagcgg ctggcgcatc catgatccct ttagttttca cgacggcgtt
     3421 tatttattac cagggtaaag agttggggtc ggctgctgcg gtagtcagtg cgctggcctc
     3481 actgtcaccg acgctagggc caactctggg gggctggatc actgataacc tcgactggcg
     3541 at
//
LOCUS       DAZUCC010000328         3526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZUCC010000328 DAZUCC010000000
VERSION     DAZUCC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.330"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..87
                     /gene="ymgI"
                     /locus_tag="ACHY09_004875"
     CDS             <1..87
                     /gene="ymgI"
                     /locus_tag="ACHY09_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFS3887714.1"
                     /translation="NNSERSLVKQILIEDEIIVCATYLIPDI"
     gene            89..433
                     /gene="ymgG"
                     /locus_tag="ACHY09_004876"
     CDS             89..433
                     /gene="ymgG"
                     /locus_tag="ACHY09_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFS3887715.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTDNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            437..772
                     /gene="ymgD"
                     /locus_tag="ACHY09_004877"
     CDS             437..772
                     /gene="ymgD"
                     /locus_tag="ACHY09_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFS3887716.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(830..3472)
                     /locus_tag="ACHY09_004878"
                     /pseudo
     CDS             complement(830..3472)
                     /locus_tag="ACHY09_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
BASE COUNT          919 a          850 c          775 g          982 t
ORIGIN      
        1 aataatagcg agcgttcttt agttaagcag atcttaattg aagacgaaat tattgtgtgt
       61 gcaacttact taataccgga catctaagtt gaagaaaaaa atacttgcct tcgggcttat
      121 ctcagcattg ttctgctcca cgccagcgat ggcggatatg aaccgcacca cgaaaggtgc
      181 attgctaggc gcaggcgttg gtttacttac tggcaatggt gttaatggcg tactgaaagg
      241 tgcagctgtt ggcgctggtg ttggtgcagt aacagaaaaa ggccgcgacg gtaaaaatgc
      301 gcgtaaaggt gcaaaagtgg gtgccgccgt tggtgctgtc actggcgttc tgaccgacaa
      361 tggcctcgaa ggggcgatta aaggtgccgt gattggcggt acgggtggtg caattctggg
      421 taaaatgaaa taaggaatga tgatgaaaaa atttgcttta ctggcgggtc tgtttgtttt
      481 cgcaccaatg acctgggcgc aggactacaa tattaaaaac ggtttgccgt cagaaaccta
      541 tattacttgc gcagaagcca acgaaatggc aaaaactgac agcgcacagg ttgcagaaat
      601 tgttgcggta atgggtaatg ccagcgttgc cagccgtgat ttaaaaattg agcaatctcc
      661 ggaactaagc gccaaagtgg tagaaaaact gaatcaggtt tgcgctaaag acccgcaaat
      721 gctgttgatc acggcaattg atgacaccat gcgtgctatc ggcaaaaaat aatatttcat
      781 atgttaccgc gtcgatgaat tcgacgcggt tttttagcta atggcgggat cagaacttat
      841 atttcaggcc caccatcatt gcggtgtcat tgtagccatt atcccccagc tgcacgccca
      901 cattacccca gacgctggcc gccggattca gattgccatt tacgccaagt ttaatttccc
      961 ccagattacg cgccccgtct tgttcaactt tcacaccgtt cattgagaca gcaaaatctt
     1021 tactgttatg gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg tgcggaccgc
     1081 tgtttttctc ctgagtacga ataaaggtgc ggaagccgag tcgggtctgg atgttgttat
     1141 ttgcgtcatt ctcaatacgt gagccattgg cttccgtgtg cgcattatga tcgacgccca
     1201 tccagatagc ctgtggctgg aagagccacg tatatttcgc cgcttccaga ccaaaggatt
     1261 tattcagggc aaaaccgtaa cccgcttcca gcgagacggt tgcacctttc gcgctatagg
     1321 attcataggc taattcatcc cctttcaccg agtttttaaa ccagctatat tacgcccagg
     1381 tgtcaatgta tgcgcctttt ttactgatgt catcggcaaa ccaggtggca tacagccctg
     1441 cgctatagcc tctgacactc cctttcgaac gataatccga cacgctggaa tgagttgagt
     1501 tgtagtcgcg ggcataacca gccaacactc ccaaacgcca actgtcactg tcggtaaaac
     1561 caccggttaa cagatccgcc cctaactgcg agacgtagcg atgcgaggtc gttctcaact
     1621 gtccgttgct gtcacgccag gcattatgcc cgccaatttg acgtagccaa agccggcttg
     1681 aacgctcctg ttaagttaca ggatcgatgt aacgcgtttc acctgcacga tcgttcagac
     1741 gcataacaaa aagagtgtta gcggctgcca ggttggcgac gtaacttcca gcttcagggc
     1801 gcaggacggg ggtaggcgca acagtaggcg ctgaacttcc tccggagttt gtttcaccgc
     1861 cgctcgttgc ctggaagtta gtcagatacc agtttttatt ggtcgcttcg gtattacctc
     1921 gggttaatga atattcgtag gcaccggcga aaatcctcga atccttaata aattctccat
     1981 ttgattcccc ctcaacgctg ataatctcta tcccatttaa cgtgtaagcc ccgctgccgc
     2041 cagcattggt aactttaact cgggttgttc cagaggtatt tccattaata acgactttat
     2101 ccgtagctga attatcatca cccaattcag ttctgagatg caatacgcca ttattaccaa
     2161 cgtaattttc agttattgtc aatcttgttg gttcgaaatc ctttccatct gcccgggaaa
     2221 tatagaccgt actattatca accgtgagtt tgctcaccgt tgagtcagct ttaatatccc
     2281 aggtactatt atctgaaagc gacagatagg tatgagtgtt atcatctgtt gaaagattgg
     2341 ctgaacccgt tatttttgaa ttagttgcat cgacggtcaa atgaccttta atattattaa
     2401 taaaaacatc tgcagccgat gatacctcac tatccgtaat ggtcacgtta ctcgtctcat
     2461 ttttatggta gaggatttca tttgctgacc gtagtgagct accagataaa atatctaccg
     2521 ttgcttcctg accatttatg gaagactcca gcacaattcc agccccctga tgcgcgactg
     2581 cagatgtatt tttcagcgtc accttgccgc cattataaat tttagcaatc gcggcgtcag
     2641 gacccgttgt ataaatttct ccctggttaa atgaaactgt cccctggcga gcagcgatgg
     2701 catgagcatt gtccccatgc gtggtgatat caacatcggt taatgtaacg ctggcattgg
     2761 tataatcaag cttgctaccc tccttgacga cagtgccatt agcgtaagcg gcataacttt
     2821 ctgttccgtg ggttgttatc tccccttttg ccatatccag ttttgctttg gcgttatttt
     2881 cattacttcc tgtatcggca gtactttctt catcaagaga agatacacac gcacgcgcgc
     2941 ttccgggaga tgcacacgca agaacgccga ctgcgtgatt acccgcagta acgataaaag
     3001 tattacctcc gtcagaaaaa gagggggtag ccgaaccgtc agggttgact cttattatgg
     3061 tatcagtatt caggtttcca tcaacttgac ttaacaaacc aacagaataa tctcccgtcg
     3121 tgacaatcgt attattaccc cgcaaagtga cagcaccgcc atattcactt acgactgcgc
     3181 tagctgtata tgctgtgctt agagaactat tgtcagaagg atataaaccg cccgttgtta
     3241 caatgacgcc attgttaatg gttaaattgc taccctttcc tgcaacagat acaccgtaac
     3301 ttccattacc cgttgttata actagcgcat tatcaatatc ttttacttct ccatctttta
     3361 tttcaacaac atgtttaggt tcatcgtgct gaataaactg ttcagcatat gcattaccca
     3421 ccgccaaagc aataagtcct aaactaaacc cggggagttt tttcagtttc atagattaac
     3481 catccattca ttggattaac aaacattcct ggtcaaataa cctatg
//
LOCUS       DAZUCC010000329         3491 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZUCC010000329 DAZUCC010000000
VERSION     DAZUCC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.331"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..337
                     /locus_tag="ACHY09_004879"
     CDS             <1..337
                     /locus_tag="ACHY09_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3887717.1"
                     /translation="PVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALINGSYCSV
                     PEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNLKRINELV
                     RHQHIDYLV"
     gene            complement(398..1426)
                     /gene="chuS"
                     /locus_tag="ACHY09_004880"
     CDS             complement(398..1426)
                     /gene="chuS"
                     /locus_tag="ACHY09_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFS3887718.1"
                     /translation="MNHYTRWLELKEQNSGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     MPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEVVTA"
     gene            complement(1475..3364)
                     /gene="chuA"
                     /locus_tag="ACHY09_004881"
                     /gene_synonym="shuA"
     CDS             complement(1475..3364)
                     /gene="chuA"
                     /locus_tag="ACHY09_004881"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFS3887719.1"
                     /translation="MTVTATGNARSSFEAPMMVSVIDTSAPENQTATSATDLLRHVPG
                     ITLDGTGRTNGQDVNMRGYDHRGVLVLVDGVRQGTDTGHLNGTFLDPALIKRVEIVRG
                     PSALLYGSGALGGVISYDTVDAKDLLQEGQSSGFRVFGTGGTGDHSLGLGASAFGRTE
                     NLDGIVAWSSRDRGDLRQSNGETAPNDESINNMLAKGTWQIDSAQSLSGLVRYYNNDA
                     REPKNPQTVEASDSSNPMVDRSTIQRDAQLSYKLAPQGNDWLNADAKIYWSEVRINAQ
                     NTGSSGEYREQITKGARLENRSTLFADSFASHLLTYGGEYYRQEQHPGGATTGFPQAK
                     IDFSSGWLQDEITLRDLPITLLGGTRYDSYRGSSDGYKDVDADKWSSRAGMTINPTNW
                     LMLFGSYAQAFRAPTMGEMYNDSKHFSIGRFYTNYWVPNPNLRPETNETQEYGFGLRF
                     DDLMLSNDALEFKASYFDTKAKDYISTTVDFAAATTMSYNVPNAKIWGWDVMTKYTTD
                     LFSLDVAYNRTRGKDTDTGEYISSINPDTVTSTLNIPIAHSGFSVGWVGTFADRSTHI
                     SSSYSKQPGYGVNDFYVSYQGQQALKGMTTTLVLGNAFDKEYWSPQGIPQDGRNGKIF
                     VSYQW"
BASE COUNT          886 a          906 c          805 g          894 t
ORIGIN      
        1 acctgtccat gtgattattt tctctccctt taatattaaa cgctgcctgg ggaaagtacc
       61 ggtgaccttt gttccgcgaa ctatcaccat catcgacttt gtcgcactca tcaatggcag
      121 ttactgctct gtgcctgaag cggatgtgtc cctttcgcgc aagcagcatc aggttctgag
      181 ctgcattgcg aatcaaatga caacggaaga tattctggag aaactgaaaa tatcgctaaa
      241 aacgttctac tgccataaac acaatatcat gatgatcctc aatcttaagc ggatcaatga
      301 gttggtacgc catcagcata ttgattatct ggtgtgaacg attgatcaat ataaagaggc
      361 ccagcaataa atagccagac ctcccgttaa ttatacgtta tgcagtaacg acttccggta
      421 tcagcgaagc aatttgctta cgccattgcg cttgctcctg ttcgccttct gtacgttgac
      481 cataaagttg cgctatctgc gtaccatcat gggcaaacaa ttccagactg gttacgtagc
      541 catcgctggt cggtttacgg gtaacccagg cttcagcaat gctctcttct aatagatgaa
      601 gagtaaacgt cgggttgaaa atattcagcc aacctttcat tggcaccact ttttctacca
      661 caccagtgaa aatctgtacg cagccacggt tgccaacaaa caccatgatt tcattgccat
      721 cctgctgtgc agattcaaga atttgcgcca acgcactgtt ggatactttg caggccaaat
      781 catctgccac cagattgaac gcctgttggc gcgtcaggtt gtggcgcttg agcaacgtaa
      841 aaaactgatg aacgtcggtc atcgcccgcc actcttgctc gaccacactg gcatcggctc
      901 gcgtttgaac aactggcgca tcaacggctt ttaactcaag cggcatattc tcatcggcga
      961 taaaccgtgc cagaagctca ctccatgccg ccatatcggt attgtcggtg gcataaactt
     1021 ttagtaatgc atcgccctga tgatcaaaga actgaatact ctggcgttcg ccacgagtcg
     1081 tattttcttt aatgtgaaaa acactggccc attgattgag aaacagacgc aaatccagcg
     1141 cgcgtggatt gaggatcaat ccggcatgtc cgttcaaatg ctggtttgtg aacgcaccaa
     1201 cttgctcatg gactgcatat tcattacgac aaatacattt ggtctcgcca acactttcga
     1261 gcgccgccag aatttcacgg atatcgccgc gcatccgcca cgcatcgtgc gtgacgcgcg
     1321 caaatgccag ttctgcttcg ctaatattca ttaaacctgc gatgtcacgc gcgtactttc
     1381 cggaattttg ttcttttaac tcaagccagc gtgtgtagtg gttcattgtc tctcttcctt
     1441 ccagataaat gccccgaaat atcggggcag atgattacca ttgataactc acgaaaattt
     1501 ttccattacg accatcctgt gggatgcctt gtggcgacca gtactctttg tcgaaagcgt
     1561 tacccaacac caaagtagtg gtcatacctt tgagcgcctg ttgtccttga taactgacgt
     1621 agaaatcatt cacgccatag cctggttgtt tgctgtaact gctgctgata tgtgttgagc
     1681 gatcggcaaa cgtaccaacc cacccaacag agaagccact gtgagcgatc ggaatattca
     1741 gggtgctggt aacggtatcc gggttaatac tggagatata ttcgccggta tcggtgtctt
     1801 tgccgcgggt acggttatag gccacatcaa ggctaaacag atcagtggta tatttcgtca
     1861 tcacatccca accccagatt ttggcgttcg ggacgttata cgacatagtt gtcgccgccg
     1921 cgaaatcgac ggtcgtggag atgtaatcct tcgctttggt atcaaagtag ctggctttaa
     1981 attccagagc atcattggac aacatcaggt catcaaaacg cagcccaaaa ccgtactcct
     2041 gagtttcgtt agtttccgga cgtaagttcg ggtttggcac ccaatagttg gtatagaagc
     2101 gaccaatcga gaagtgctta gaatcgttat acatttcgcc catcgtcggg gcgcggaatg
     2161 cctgagcata tgagccaaat aacatcagcc agttagtcgg gttgatagtc atccccgcgc
     2221 gagatgacca tttgtcggca tcaacatctt tgtagccgtc gctgctaccg cgataactgt
     2281 catagcgggt tccgccaagc agggtaatcg gcagatcgcg taaggtgatc tcatcttgta
     2341 gccagccaga gctaaaatcg atttttgctt gcgggaagcc cgtcgtcgcg ccacccggat
     2401 gttgttcctg acgataatac tcaccgccat atgtcagtaa gtgagaagcg aaactgtcgg
     2461 caaatagagt ggaacggttc tccagccttg ctccttttgt tatctgttca cgatactcgc
     2521 cggaactccc cgtgttttgc gcattaatac ggacttccga ccagtaaatt tttgcatctg
     2581 catttaacca gtcgttaccc tgcggggcga gtttataaga aagctgcgca tcgcgttgaa
     2641 ttgttgaacg atcgaccatc gggttgctgc tatcagaagc ttcaacggtc tgcggatttt
     2701 ttggttcacg cgcgtcgttg ttgtagtaac gcactaaacc gctcagagac tgagctgaat
     2761 caatttgcca ggtccctttc gccagcatgt tattaatgga ttcgtcattc ggcgcggttt
     2821 caccattgct ctggcgtaaa tcaccccgat cgcgactgga ccaggccaca ataccatcca
     2881 gattttcagt tcgcccaaac gcgctcgcgc ctaatcccag gctatggtcc cccgtgccgc
     2941 cagtaccaaa gacacgaaaa ccactgcttt gtccttcctg caataaatct tttgcatcga
     3001 ccgtatcgta ggagatcact ccacccagcg cgccactgcc atacagtaat gctgaaggtc
     3061 cgcgaacaat ctcaacacgc ttgatcagcg ccggatcgag aaaagtgcca ttcaggtgtc
     3121 cggtatccgt tccctggcga acaccatcga caagaaccag cacgccgcga tgatcatagc
     3181 cacgcatatt tacatcctga ccgttggttc gtccggtacc atcaagagta attccaggaa
     3241 catgacgcag cagatcggtg gctgaagtag ccgtttgatt ttcaggagcg gaagtgtcga
     3301 tgacgctgac catcataggc gcttcgaagg aactacgggc attccccgtt gccgtaacgg
     3361 tcatggtttc agtagcaaaa gcaaacgttg gcaaggtggc agaaacagcc aaagccaaca
     3421 aactcaaacg caacgaggta aattgcggac gtgacatagc gattctccat gaggaaaata
     3481 aaaaatgctg g
//
LOCUS       DAZUCC010000330         3483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZUCC010000330 DAZUCC010000000
VERSION     DAZUCC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.332"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..219
                     /locus_tag="ACHY09_004882"
     CDS             <1..219
                     /locus_tag="ACHY09_004882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887720.1"
                     /translation="KKTTHPNHLIIRFLTDFLNNKISFAGGWSHCVTPTTITSVFSRT
                     VVNHKIGTHAGGAGPHDFLFDESENGER"
     gene            complement(1373..1567)
                     /locus_tag="ACHY09_004883"
     CDS             complement(1373..1567)
                     /locus_tag="ACHY09_004883"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFS3887721.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYTGAWKKWHDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            complement(2141..3157)
                     /locus_tag="ACHY09_004884"
     CDS             complement(2141..3157)
                     /locus_tag="ACHY09_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFS3887722.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYESDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLVERPAARWLFMTLTVKNPPVENTRETLIRMNAAWKRLSDRKEFRPVLGWLRTTE
                     ITYGRVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKQPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLVGADIPAEPDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
BASE COUNT          900 a          936 c          739 g          908 t
ORIGIN      
        1 aaaaaaacaa cacatccaaa ccacctgatc atcaggtttc tcaccgactt tctgaacaac
       61 aaaatcagct ttgcaggtgg ttggtcccac tgcgtgacac caaccaccat caccagtgtt
      121 ttttctcgta ccgtcgtcaa tcacaaaatc ggaacccacg ccggaggcgc gggaccccac
      181 gattttctgt ttgacgagtc agaaaacggc gaacgctgac agatttcaat ctggctgcgt
      241 gagacgttgg cggggtgtcg gggcgcagcc ctgaccaagt ggtaatcgta tagccgtgcg
      301 tgcgcggtta tacgattaca catcctgtcc cggaatcagg attgtttttc gcgcacaact
      361 gcggcaaatt caccccgttg cggttgcaac gatgggaata tcaaaactac gaaaattatt
      421 caggcatttg ctacccctct ggccctggcc tccacctgtt ccggggggga tttttttgtt
      481 gcctctcatg acgaactgcg gcaaatttct gacgttgcaa actgcaacac ataaaacaca
      541 cagaaaaact cacaattgcc atgtcctcat tgacaccccc ttgccatata gggggatttt
      601 ttcgcctgtc ctgattgtct tttgctgata gttacggcag attggaaaag agcgcatttg
      661 tggtgctctc tccaaaataa tatttctcat tactcaaact ctgatggctt ccccctgtaa
      721 tccaggggga tttttttaat ttagcgctat gcttcgtaat tcattcagaa tacagacagt
      781 attgttaatc agtttttcat catgaacggt gtgttcagca ataagagtat taaactcaat
      841 aatcaggctg taactctctc tgtatacatc atctctgtta aattcgttcg ctctgtattt
      901 tgaataaata ataatgcctt tgtcctgtat actctgaatt tgtttaatta catcaatact
      961 cataaaaaca tccaacaaga accgactgta ggtcaccggg caaacgagcg aaatgctagc
     1021 attttgcggc gtttgcccta tcctgcatca gaacgctaaa tacaggaatc tctttatgac
     1081 gattcctcca tttcaagatt ttttatgtgg cgtctactac atgcgatccg ttggtgaacc
     1141 gttagttagc ctttagatgg accttagatg gacactagtt agatatttac tgatttagat
     1201 gaaccttaaa tggatcttag atggatatta gatggatctt agttagacat tatgtagata
     1261 ggaaatatta tggggcaatc ctggaggcac gtgaccgact gtatacaaaa tgctggcgca
     1321 ttttgatacg tcgacgtgcc aaaggggacg cccctttgaa accccgccag aattaaaacc
     1381 ggcctgtctt tttacaatat tcagctctgg ctatacacca gtctatattt tcgcctttgg
     1441 cataatgaag cgcatcatgc cattttttcc atgctccagt gtaatcgcct tcacgctcca
     1501 gtctgacagc ttcgtttgcg ggtgaaaaat atttattttc taaataaatt gctttgtcct
     1561 gattcatact cgtaatttat atttttattt gaattaaaaa agagcggcct gtcgggttaa
     1621 tacatactgg catgactgtt gatatatcag cccaactgca cgctcgtaat cgttttaacg
     1681 tgattttcgc aacatggcaa tccgggttca tctgtccggt aatcaataaa aaccgtgcga
     1741 aaaacgggac aggatgtggg aatgtcggac cgcgcacgcg cactcagaca ttccccacct
     1801 ggtcagggct gcgccccgac accctcgaca ccgttcccgc aatacgatag aacatcaggg
     1861 cgcgtttccg ggtaaacgca tgttaatgac cgggaattca ttgattaggg accggagacg
     1921 cgctacaaaa ctcctgcgcg tttctggcat caactgacca tggggatggt ttggcagtag
     1981 cagcaagcgc cagaaacgcg ctcaaaacgt ctctgacggc ctgaatccaa caaaacatac
     2041 aaaaccattg cccgatgcaa aaaagtgcgt tagaaagcgt ctgagcgcgt ttctggcgtc
     2101 ttttttgttg ctgtgtcgct actcgttgtc agattcgcgc tcaatccttc atcctgtcag
     2161 cttcaggatt ccgcttgtaa cgtttttccg tacgtcccca gtcaaacgcc agccagaagg
     2221 cttgctcctc gaactcgtcc ggttctgccg gaatatctgc cccaaccaaa tcctcgtctg
     2281 tctccggttt atcgactttc agaacctctt tgagacgacc gccggttgcc accatccgaa
     2341 gcccgtaggt ctgccgcgcc agctccagaa accacgccgg atcacgcaca acttccgaag
     2401 atttcaccgt gtatttcatc gtctcaatga cgcctgactc cagcgccgca cgatgcatat
     2461 caggcagggt tacaccatcc ggctgtttcc agccctgttt cggttttacc acccgaatat
     2521 ccactcccgc ctcataatcg acacgaaggc attcactcca gatctccacc cagcgagcat
     2581 gtttcacata accattaccg gacaacatcg aaggggggac catcatcaga acgtgaaaat
     2641 gcggatggca acatcctggc actcgcccgt aagtaatttc cgtcgtacgt aaccagccaa
     2701 gaaccggcct gaattcctta cgatcactca agcgtttcca tgctgcattc atccgaatga
     2761 gtgtttcgcg ggtattttcc acagggggat ttttgacagt cagcgtcatg aacaaccagc
     2821 gggccgcagg acgttcaacc aacagctttg gcaaggcctc ccagagacga cgcatccaac
     2881 ggagagagcg acgacgacta cacaccggac aaaacggaac atgacaaaac tcccccttcc
     2941 gaagagccag cttcatttcg cccgtcaggc gggacacctt cggcgcaaac aacaaccgac
     3001 gggaacaatc aaacatccgc tctccacgag aggaaaacat cgaatcgctt tcgtaaatct
     3061 gcgccaccct gtcagccatc acgcgatcgc tgtcccattt tgtatcccgt gtgctgtaat
     3121 cgcgcaacga gagtaccgga ctatctggat ttttcatttg caccccccaa gaactgggca
     3181 gatgcggaaa ttttgtgtta gcatttttct gtcacctgtg actctgttgc cgaacaacga
     3241 attccaaact ggtgatcgtc ccctggagtg tgaccgcact cgcaggggaa ccccctcaaa
     3301 actgttcatt ttttaaacaa acatttttat ctcttttcca cccattttta ccgctcaaag
     3361 ccgcgccacc actggcctaa gccaaaataa cacagaccgc agttgttgcc tatagtatat
     3421 atacaaggat cgcgcgcacg atcgcccacc aaaaatgcgc gttttttgag cgcttccgag
     3481 cac
//
LOCUS       DAZUCC010000331         3439 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZUCC010000331 DAZUCC010000000
VERSION     DAZUCC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.333"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            64..324
                     /locus_tag="ACHY09_004885"
                     /pseudo
     CDS             64..324
                     /locus_tag="ACHY09_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            306..392
                     /locus_tag="ACHY09_004886"
                     /pseudo
     CDS             306..392
                     /locus_tag="ACHY09_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            389..1540
                     /locus_tag="ACHY09_004887"
     CDS             389..1540
                     /locus_tag="ACHY09_004887"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFS3887723.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            1547..2447
                     /locus_tag="ACHY09_004888"
                     /pseudo
     CDS             1547..2447
                     /locus_tag="ACHY09_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            3190..3417
                     /locus_tag="ACHY09_004889"
     CDS             3190..3417
                     /locus_tag="ACHY09_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFS3887724.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
BASE COUNT         1040 a          732 c          830 g          837 t
ORIGIN      
        1 gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta
       61 ataatgagca gaaaaaccca acgttactct aaagagttca aagccgaagc tgtcagaacg
      121 gttcttgaaa atcaactttc gatcagtgaa ggcgcttccc gattatctct tcctgaaggc
      181 actttaggac aatgggttac cgccgccaga aaagggctcg gaactcctgg ttcccgcacg
      241 gtggctgaac tggaatctga aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt
      301 gagcgagata tattaaaaaa agcaactgta gattcaattg gtcaacgcaa cagttatgtg
      361 aaaacatggg gttgcggagg ttttttgaat gagacgaaca tttacagcag aggaaaaagc
      421 ctctgttttt gaactatgga agaacggaac aggcttcagt gaaatagcga atatcctggg
      481 ttcaaaaccc ggaacgatct tcactatgtt aagggatact ggcggcataa aaccccatga
      541 gcgtaagcgg gctgtagctc acctgacact gtctgagcgc gaggagatac gagctggttt
      601 gtcagccaaa atgagcattc gtgcgatagc tactgcgctg aatcgcagtc cttcgacgat
      661 ctcacgtgaa gttcagcgta atcggggcag acgctattac aaagctgttg atgctaataa
      721 ccgagccaac agaatggcga aaaggccaaa accgtgctta ctggatcaaa atttaccatt
      781 gcgaaagctt gttctggaaa agctggagat gaaatggtct ccagagcaaa tatcaggatg
      841 gttaaggcga acaaaaccac gtcaaaaaac gctgcgaata tcacctgaga caatttataa
      901 aacgctgtac tttcgtagcc gtgaagcgct acaccacctg aatatacagc atctgcgacg
      961 gtcgcatagc cttcgccatg gcaggcgtca tacccgcaaa ggcgaaagag gtacgattaa
     1021 catagtgaac ggaacaccaa ttcacgaacg ttcccgaaat atcgataaca gacgctctct
     1081 ggggcattgg gagggcgatt tagtctcagg tacaaaaaac tctcatatag ccacacttgt
     1141 agaccgaaaa tcacgttata cgatcatcct tagactcagg ggcaaagatt ctgtctcagt
     1201 aaatcaggct cttaccgaca aattcctgag tttaccgtca gaactcagaa aatcactgac
     1261 atgggacaga ggaatggaac tggccagaca tctagaattt actgtcagca ccggcgttaa
     1321 agtttacttc tgcgatcctc agagtccttg gcagcgggga acaaatgaga acacaaatgg
     1381 gttaattcgg cagtactttc ctaaaaagac atgtcttgcc caatatactc aacatgaact
     1441 agatctggtt gctgctcagc taaacaacag accgagaaag acactgaagt tcaaaacacc
     1501 gaaagagata attgaaaggg gtgttgcatt gacagattga atctacaaca gcgtattttg
     1561 cacaggagtc gctgaaaaat acgcgttaat cgaacaatgg cgacaacaat ttcccattga
     1621 agcgatgtgt caggtatttg gtgtatccag gagcggttat tacaactggg tacagcatga
     1681 accctcagac agaaaacaaa gtgatgagcg gctaaaactg gagattaagg tggcacatat
     1741 ccgcactcgc gaaacatatg gaacccggcg gctccagacg gagctggcag agaatggcat
     1801 catcgttggt cgtgaccgac tggcacgtct tcgtaaggag ctaaggctac gctgtaagca
     1861 gaaacgcaag ttcagagcga ctacgaactc gaaccacaat ctgccagttg cgccaaatct
     1921 gctgaaccag acgttcgctc ctacagcacc aaatcaggtc tgggtggcgg acctgacgta
     1981 tgttgccaca caggagggat ggttgtacct cgctggcatc aaagatgttt atacgtgcga
     2041 aattgtcggc tacgccatgg gagagcgcat gacaaaagag ctgacaggta aagccctgtt
     2101 tatggcgctc aggagccagc gcccacctgc cgggcgggct aatccaccac tctgagcgag
     2161 gttcacagta ctgcgcatac gattaccggg tcatacagga gcagtttggt ctgaaaacat
     2221 caatgtcgcg taaaggtaac tgttacgaca acgctccgat ggaaagcttc tggggaacgc
     2281 tgaaaaatga gagcctgagc cactatcgtt ttaataaccg ggatgaagcc atctcagtaa
     2341 tacgggaata cattgagatt ttctacaatc gtcagcgtcg tcactctcgt ctggggaata
     2401 tctccccggc agccttcagg gaaaaatatc atcagatggc tgcttaaaaa aagaacaaat
     2461 ggtagtgtcc gctattgcca gtacacctca gtatagtggt atgcgtgaac atatatagtg
     2521 gcattttgtc tgatgttgtt aaggtttttt agcaggtcta cagtcaactc catcgcggta
     2581 tccttcaggt tgttatcatt cgttgatgat gcgatctgtt gaaggcgttg gcgacaatta
     2641 attctttttc tcttctgaaa aatttacgga atacaaaaaa gatctgtttt taactgattg
     2701 ttactttctg aagatattct ctgctccatt tgctttccgg gtaagacgta tggctggtta
     2761 tggtatggtc ggactgcagc cacaggggga tgactgtaat acctctgatc accacagtca
     2821 gtcatctgta aatgttaaat acctcacagt tcagtcagtg gattattgtc ggtcttacgg
     2881 gatatttgtt aaaaaaatac tgcatggtga atccccctga gcggcggggc atatcagtat
     2941 cgtaggtgtt tggtcacatc ccttatctgt gcgggttcag gtgctgatac tgaactcacc
     3001 gggaggcacc cggcaccatg ctacataaca ctccattacg ctacactcgc ccctctccgg
     3061 aggggttttc tctgcaggat gtgaatatga gatgctgcat tattcactgc gaatattgat
     3121 ggtgagaatc tttaataata ttaatgttta tatgtattgt tacagtgttg atgaaaggag
     3181 ataaaaacaa tgttcccgga gtacagagaa ctgatttcag cattgaaagg aaatcataaa
     3241 cgctttgatt ctttatttga aaagcataat aaacttgatc acgaaataaa aagaattgaa
     3301 gatgaatttg gttcagcgta ttcgacgaaa gtggtcaggc tgaagcttga aaaacttgag
     3361 atcaaaagag aaatccagaa aatccttgaa gatgagtcag aaaagggcga acggtaatca
     3421 taaacggtat attcagaga
//
LOCUS       DAZUCC010000332         3438 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZUCC010000332 DAZUCC010000000
VERSION     DAZUCC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.334"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..288)
                     /locus_tag="ACHY09_004890"
     CDS             complement(<1..288)
                     /locus_tag="ACHY09_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3887725.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAF"
     gene            complement(288..1256)
                     /locus_tag="ACHY09_004891"
     CDS             complement(288..1256)
                     /locus_tag="ACHY09_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS3887726.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(1258..2916)
                     /locus_tag="ACHY09_004892"
     CDS             complement(1258..2916)
                     /locus_tag="ACHY09_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS3887727.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            complement(2950..3318)
                     /locus_tag="ACHY09_004893"
     CDS             complement(2950..3318)
                     /locus_tag="ACHY09_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887728.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
BASE COUNT          810 a          932 c          780 g          916 t
ORIGIN      
        1 gaaagcaatc tggaaaggat caacattaat ctcctgcaaa taacgtccat accgttcact
       61 cgcttcttcc ggcgttattt tgagtttctc cagtgctgct tcggctgcgt ccagatgtac
      121 ggcctcgttc aggtgaataa cctccagaat ccagagataa gcatcagtct gtttatgtcc
      181 ggtgatcctc ctttgttctg gcagaggttt gatatttgcc ggaacggcgc tgtacgctgc
      241 cgtcgggata gtgaataaag ctttatgtac ctgattgtcc gtacgcatca cgcaaccgcc
      301 ttttttctgt aaaaaaccag ttcccgaacc tgatcgccgt tcatgagcat attgttgaaa
      361 tcatcatgat caggccaccg gacacttacc cgctgaatgt catttcgggc ctggagattt
      421 gcatgtgcgc attcacaggc cgcagccatt ccggtagcac tgtattcatc gcgatcggca
      481 aaaataatca gatgggtgac tcccgccggt acgcggaact ttttcataaa accactgtta
      541 atggttgccc aggtatttac tttgtaaatc tgatgtgcgg acaacgcggt ttcgatacct
      601 tcagctatac ccagtgtact ggctaccgga aacatcctga tagcaactga ccgggcatgg
      661 tccagatagt tatcgtcctg taaagatttg agccttctgg cgctgatacc aatgtctgct
      721 tttttagctc catcaagcaa tgtctgatgc aggtaacaca gttctccctt gtcatcggtc
      781 gccagggcat aaagagactg gaacatgcgc ccgttataat tttgctggct gttgaagcga
      841 actccctcaa ccggaagact gaatatgcca cgggaacgaa gatattcagc accagatgtt
      901 ccctgtagcg ccgtgagcgc tgaaaatcga cgctgaacgc gcgatctcag gctttccgac
      961 gtacagatca caggcggttt atctctccgg tattcattcc ccgtgatgcg gtctatttca
     1021 gcgcaaattt catgaaacgc tcttttttgt gtcagagcga tcagtttcat gccgtcaccg
     1081 ctaccacaaa cacagatcca cgttccggtg ccatcacggt catcaatacg gaacttacct
     1141 ctggcaccac aaaccggaca ttcccctttg aagtgctttt tacccgtgac tggcggcagt
     1201 ccgtaatgtt ttaaaatttc aggccagtgg cctttcgctg cttctgctgt tttcatctta
     1261 ctgactcaga ctgtttctgt ttttctgaag ttgttgcttt gcctgcatga tctgccacga
     1321 ctcactgcct tccggcaccc cagaccggac accatgaagc agggacagag aaagcgcttc
     1381 gggggatttt tccgtcatct tctctgcccg gctacgccct ctggcaaacc ggatgaattt
     1441 gtgtctgatg taattactga cctccggggt gatttccatc ggaaaactgc tcagcccgtc
     1501 aggccattcg ccgaacttct cccggaaagt gtgagcgcac catccatcgc tgaccggtcg
     1561 ccccagagag gcacgctgac gctgatagaa tttgatctga ctccaccagg cctgtttgtc
     1621 gcttttggtg taaatttttt cgtccttgct gagtttcctg atattgcggc gggtatctgt
     1681 gttcacatcc tcaccagcca gaggtttaaa tccgcatttc ggacagacat aaacacctgc
     1741 cggtttcatg aaatgacacc ccggacattc tttcggcagt ttttcttccc gaatttcgtt
     1801 accacgtgat gatgacacct gcatcccgtc gtttttgccg ggcagttcgt catactcaat
     1861 attttcagga aatcccaggc gatgaaccgt accggaatgg tcgaaaatca ggcatgactc
     1921 cttgcctggc gctgtacgca atcctctccc aatcgcctga agccagcgaa tctctgattt
     1981 tgtcgggcgt gcgtaaatga tgcaccggac atcactgtca aatcccgcca ccagaacgcc
     2041 aacgctgaca atgattttcg tcgctcccgt ttcaaaacga ctgatcatta actgacgctc
     2101 atcatgtggc gtttcagcaa ccatcacctc tgcgttaatt ccggctttgt tgaactgcat
     2161 ggtcacgaag ctggcatgcg ttttattgac gcagaacgcc actgtcggta aatcccttcc
     2221 gttacacagc cagttatcga caatatcgcc aaccagatcc gcgccgcaca taatctctgc
     2281 cagttgtgat tcgtcgtaat ccctgccgta ctccattgag actttcgttt ttacaccttc
     2341 cagatccggt ttcgtgggcg cataaaattc gtaattgctc agttcgccac gctggatcag
     2401 ttcccctata gtggtcggct tgagaagacg ctggtaataa cgccccagga acgatgaaaa
     2461 gggtgtaccg gacaacccaa tcaccttcac atttccctct gcaaccagtc gttcgatttc
     2521 cgtgaggatg cggcgcttac ggagatgcgc ttcatcaata atcagcaggt cgatattctg
     2581 aggaaaatca cggcgaatca gcgtatccgc actggcaatc tgaatcagtc gtgacgggtc
     2641 gttattggga tgatcacgcc agataaagct gatttcatcc ccggaaagcc cgtactgaac
     2701 aaagcgctgt gctgtctggt tgatcaaaat ggtataagga acgacaaaca gaacgcgcat
     2761 gccacggctt accagaccag cagtgatgaa cgccgccagg ccggttttac cactccctgt
     2821 aggcatgtac atcatgaagg actgatatgt tttccagtca tgacgcaaca tattcagcgc
     2881 acgttcctga gcaaaattcg gcttgatatt cagcatggta ttctcccgat gacagggctg
     2941 atacagtgat tacttaaacc gggtcgcccg gatgacatcc acaccatcgg cgatataacg
     3001 ctgtggatgt ttgccatcac ggagaaaaac aaatcccgaa atgccttcag gaagcgctcg
     3061 aattttcatc agtgaacgct gacggggcga tcggttatcc acctcaactg cgcaacgctg
     3121 accatcggga gaaataacca gaagatcgat aaatcccctg cgatcacctt tcagcctgac
     3181 agtgaaacag cgttgcaact cacagcccag ggatttgatt ttcttttgca gaacgtagtc
     3241 gaatgtgacc ttgtccattc catgtccgaa ttcctcttcc agaaggttga ttagtaccag
     3301 tttcagttca gcgctcatat tttaaccatg atgtactagt ttgctgatac gaccgttttt
     3361 tcaggggtgc ccccctttag atcgagatct acctaacctc tgtacccgtc tgttggaaga
     3421 tcccgttcca gtgcttcg
//
LOCUS       DAZUCC010000333         3435 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZUCC010000333 DAZUCC010000000
VERSION     DAZUCC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.335"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(7..633)
                     /gene="dsbA"
                     /locus_tag="ACHY09_004894"
     CDS             complement(7..633)
                     /gene="dsbA"
                     /locus_tag="ACHY09_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFS3887729.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(650..1636)
                     /gene="srkA"
                     /locus_tag="ACHY09_004895"
     CDS             complement(650..1636)
                     /gene="srkA"
                     /locus_tag="ACHY09_004895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFS3887730.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTPDQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1713..1982)
                     /locus_tag="ACHY09_004896"
     CDS             complement(1713..1982)
                     /locus_tag="ACHY09_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFS3887731.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2052..2636
                     /gene="mobA"
                     /locus_tag="ACHY09_004897"
     CDS             2052..2636
                     /gene="mobA"
                     /locus_tag="ACHY09_004897"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFS3887732.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWIHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            2618..3145
                     /gene="mobB"
                     /locus_tag="ACHY09_004898"
     CDS             2618..3145
                     /gene="mobB"
                     /locus_tag="ACHY09_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFS3887733.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPVLCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALMDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(3248..3363)
                     /gene="rrf"
                     /locus_tag="ACHY09_004899"
     rRNA            complement(3248..3363)
                     /gene="rrf"
                     /locus_tag="ACHY09_004899"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          892 a          841 c          828 g          874 t
ORIGIN      
        1 attgaattat tttttctcgg acagatattt cacagtatca gcatactgct gaacaaaaac
       61 atccatattg ctggtatcca taccctgcgg attcagctga tatttaccgt taacaaacat
      121 cgccggaacg ccacgcaatt gcacgtcagc tgcagctttt tcctgctgag cgaccaggga
      181 tttcaccacg aagctgttcc acgctgcgtc gtactcttca cctttaatac ctgcgttgat
      241 aaatacatcg cggatatcag atgctgaacg aatggtctgg gttttctgta cgccttcaaa
      301 cagcggaaca gtgactttgt cttccacgcc cagtgccatc gccactgccc atgcctgagt
      361 cagatctttg cccaggtcac cccccatgaa gttgacgtgg tatttagtca tcttcacgcc
      421 ttccggcagt tttttcttca cgttatcaga aatatgcaga acttcttcaa actgatagca
      481 gtgcgggcag aagaaagaga aaaactccag cacttgcggc gcgccagcaa ctggtttttc
      541 cagggtagtg tactgtttac catcttcata ctgcgccgcc gatgcgctaa acgctaaaac
      601 taaaccagcc agcgccagcc aaatcttttt catgatctac tctctccgat taatacatag
      661 gtgttaattg caaagggggt tcttgtagaa cttttgcctg ttctataaaa gtcgccgtct
      721 gtcgcagcca gtaatcttcc ccggttaacc acgggaaatt tttcgggaac gcgggatcag
      781 cccaacgccg cattagccag gcaagataat aaaccaaacg catggcgcgt aaaggttcaa
      841 tcagtccgat ttcagcggtg tcgaactcgc taaattcttc ataagcttca ataatagttt
      901 ccagttgcat ccgctgctcg gctttatcgc cattgagcaa catccacaaa tcctgaatag
      961 ccggaccatt acgtgcatca tccagatcaa caaacattgg accatcgcgc cagagaatat
     1021 tcccggcgtg gcagtctcca tgtagccgca gaacggtgaa atcttcccgc cagtgtgctg
     1081 taacggcggc gatcagctca tctgtcgctt tcagaaatgc cgctttcaac ccggaaggta
     1141 tcagtgtagc gtcctcaaac agcttgcgag gctcaatgag atattcattc agaccgatgg
     1201 tcgggcgatg gataaaaagc tgtttgcgcc ccgtctggtg catacgccct aaataacgcc
     1261 caaccgcttc catctgatcg atattatcag cttcgaactg gcgaccaccg acgcttggaa
     1321 aaacagcgaa ataaaatccc tgatgattca ataaagtctg accgttaaag gccacaggtg
     1381 ctgcgaccgg aacttcatca tttaccaact gcaacgcaaa ttgatgttct tcgaggattt
     1441 gatcgggtgt ccaacgttca ggacgataaa atttgacgac aaaacgtcga cgatcttcgt
     1501 cctgaaattg atagacacgg ttttcatagc tgttaagcgg ggtaagaccg gaatccaccc
     1561 ggatcccttg ctcaaacaga gcatccatga tggtatccgg gtgtagtgtc tggaaagtaa
     1621 aagcgctgtt gttcatccca tcatccggaa aacattacga atgatgaagg atatcatttc
     1681 acgatgattt tggtggcacc gcttacaagc ttttactctt taattacgcc gcgagcgcgt
     1741 aacagcgccg ttttgaagtc ttcttcataa tctttctgca aacccgggat caccgcatct
     1801 ttcgcggaat cacgcatttt caggtgatag atcagaatgt catccgtcaa atccgccagt
     1861 tcgccgtcaa aacctgactc tttcgccagt ttctgcaaaa attgcagcag gttaaggtct
     1921 ggctcttttt gccaggctgg ctggaggagt tcaataactt cattcagacg tttacatttc
     1981 atagtaatgc tccttactct tgagacagac acgttagcag ggtcaatccc acaataaaag
     2041 aggcgatatc ggtgaatctg atgacgacga taacaggcgt tgtgctggca ggcggtaaag
     2101 ccagacgaat gggcggcgta gataaaggat tgcttgaatt aaacggcaaa ccattatggc
     2161 aacatgtcgc tgacgcgctt atgacgcagc tctctcacgt cgtggttaat gctaatcgtc
     2221 atcaggaaat ctatcaggca agcggtctga aagtgattga agattcactg gcggattacc
     2281 caggccctct ggcaggaatg ctttcagtaa tgcagcagga agcgggtgag tggtttttgt
     2341 tttgtccgtg cgatacgcct tacattcccc atgatttagc cgcccggctt aatcatcagc
     2401 gcaaagatgc gcctgtcgtg tggatccacg acggtgaacg cgatcacccg actattgctc
     2461 tggtaaaccg cgcaattgag cctttattac tggaatatct gcaagcagga gaacgccggg
     2521 ttatggcatt tatgcgtctg gctggcggtc atgcggttga tttcagcgat cgtaaagagg
     2581 catttataaa cgtgaatacg ccagaggagc ttgcccgatg gcaggaaaaa cgatgatacc
     2641 gttactcgcc tttgccgcgt ggagtggtac tggaaaaact acactgctga aaaaactgat
     2701 cccggtatta tgcgccagag ggatccgtcc gggactgatt aagcatacgc accatgatat
     2761 ggatgttgat aaaccgggca aagatagcta tgagttgcgc aaggctggcg cggcacaaac
     2821 catcgttgcc agccagcaac gatgggcctt gatgacggaa acaccagacg aagaagagct
     2881 ggatctacat tttctcgcaa gtcgaatgga tacctcaaag ctggatttga ttctggtcga
     2941 agggtttaag catgaagaga tcgcaaagat tgtgctgttt cgcgatggag ccggacatcg
     3001 accggaagaa ttagtgatag acagacatgt tattgctgta gccagtgatg tgccgcttaa
     3061 tcttgatgtc gcgttaatgg atattaatga tgttgagggg ctggctgatt ttgtggtgga
     3121 gtggatgcaa aagcaggacg gataagcaaa agtaggctgg ataagacgcg ccagcgtcgc
     3181 atccggtatt tattctgctc cagcgcaaaa aggccatcct gacggatagc cttctgctta
     3241 atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct
     3301 acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc taaggccgcc
     3361 aggcaaattc tgttttatca acacgtctcg ttatgacatg tcgatttaat ctgtatcagg
     3421 ctgaaaatct tctct
//
LOCUS       DAZUCC010000334         3420 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZUCC010000334 DAZUCC010000000
VERSION     DAZUCC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.336"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..110)
                     /locus_tag="ACHY09_004900"
                     /pseudo
     CDS             complement(1..110)
                     /locus_tag="ACHY09_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(550..1392)
                     /locus_tag="ACHY09_004901"
     CDS             complement(550..1392)
                     /locus_tag="ACHY09_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS3887734.1"
                     /translation="MSDITVSRPEVVNWHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLEIMLHLFSGKPIPDNR
                     ENITIHLDDHVQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAI"
     gene            complement(1477..1674)
                     /locus_tag="ACHY09_004902"
     CDS             complement(1477..1674)
                     /locus_tag="ACHY09_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFS3887735.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQLQLLHQNR"
     gene            complement(1686..1925)
                     /locus_tag="ACHY09_004903"
                     /pseudo
     CDS             complement(1686..1925)
                     /locus_tag="ACHY09_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            1992..3154
                     /locus_tag="ACHY09_004904"
     CDS             join(1992..2253,2253..3154)
                     /locus_tag="ACHY09_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFS3887736.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            complement(3151..>3420)
                     /locus_tag="ACHY09_004905"
     CDS             complement(3151..>3420)
                     /locus_tag="ACHY09_004905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887737.1"
                     /translation="YLNINEQEVFKMREISDNMLDSVKGGMNLNGLPGSTNVIDLRGK
                     DMGTYIDANGACWAPDTPSIIIYPGGSGPSYSMSSDPTHVIWTQA"
BASE COUNT          835 a          820 c          857 g          908 t
ORIGIN      
        1 attttaatgg attgttagtc tttgcatgag caaacggact gatacatttc tctttgttct
       61 cattcagaaa atctcatcag ttggcgttct gcccgatgtt gtgctttatg ggtgcgttac
      121 gctcctgatg acgtcatttg acgttcaaca gcatcacggg gccgcacgac atttcacgtc
      181 agtcagcgct atagctcaga aacaaatttt cccgaattgg aatatgcccg caaattgctg
      241 gggatgtggg gagaatctgg ttgagttcgg tagaattgat ttggcggcag gacgcttaaa
      301 tcgtggcgtc ctgaaacgaa aaacggacct ccgtggaggt ccgtttatat gaatttggtg
      361 ccggactcgg aatcgaagat ttcatttatc atattgatat taatctcgtt tatttggttt
      421 gatgaaaaat gtaccctctc ttgtaccctc ggtcccttct ggtggttttt atgagaacaa
      481 gtagatcaag atttttgtca tcagaacacg tgtatttttg tgggttttat tcctgagttt
      541 tattcctttt tatatcgcta acgctccggg atagtgctta gcaataatat cattaagcct
      601 gtcaaccagc tctggtttcc tgaacgtgat gtgcgcggag cccttcttaa agtatctgat
      661 gctgaacatc tcatcttcat agtcctcttt accctgaaca gactggacgt gatcatccag
      721 atgaatagtg atgttttccc ggttgtcggg gatcggtttg ccgctgaaca ggtgtagcat
      781 tatttccaga tcagcaagtc gatctgcctg ttgcccggtg ataagatgaa agccccaccg
      841 gtcccacctc accaggttgt tgacgataat tttactgcca aatttgcagg ggcaattggt
      901 tttgtaattc cagctcaacc ctctgaagac gttgatcacc ccccgctcaa acacctcatc
      961 cttattctgg tgcagttgtt caaatgtgct cagaatgttc gcttcactga tttccggaaa
     1021 attatcgtat tccagtgacc ggtaccatgt atcacgggcc tgtgcatcca ttaaggaaag
     1081 catcccggaa cgttgcatca ggtcacgcca gatttcgcga tcgagattgc gtgtgatggc
     1141 tttcattgcg gtttcaggtt tctccatcag ccagcaaccg cagtgaaaat tctgtttcat
     1201 ggcccagtcc agtgcggttt taccgccaat gctttctgtc attgctgaga tttcagccag
     1261 ttgccggata agtgtgtcga tttgcagcag tgtactcttt cgtacagcca gaatgtggag
     1321 gatactggtt gagcagataa cgtccgtatg ccagttgaca acttccggac gggagactgt
     1381 gatatcagac ataatcgata ttcctttttg cagataaatg aaaacgccag ccctgttcag
     1441 gctggcgtca gtggaatgaa gtctggtgag tgagagtcac cggttctggt gcagaagctg
     1501 caactggcgc agtgtatgga tatcttccct ggcctgttca atgcagggca gcagtgctgc
     1561 cgaatccgtt ttatcctcat cgttgtcaaa gataattccc gattcgcagt cgatattgtc
     1621 ctgcagccac gcaatcagaa tatccagcgc tgtttccgtg gttaatgatt tcatgttgtt
     1681 tctggttacc agtcgaaagt gggtaaaccc ggcaggcatt cagcgctggc atccagatga
     1741 atgagactga cgccataacg tcgggtaagt gtgacaacca gacggcggaa cgtttttgac
     1801 agccccaggc gtttaagccg cagaaccggg tatgaccacg catcggtacg taacagatat
     1861 ccgctacctg tgaaatgaat ccattctgat tcgccggtgt cattcgtctg atgtgacagc
     1921 gagtactgat cttacccagc aatagtggac acgcggctaa gtgagtaaac tctcagtcag
     1981 aggtgactca catgacaaaa acagtatcaa ccagtaaaaa accccgtaaa cagcattcgc
     2041 ctgaatttcg cagtgaagcc ctgaagcttg ctgaacgcat cggtgttact gccgcagccc
     2101 gtgaactcag cctgtatgaa tcacagctct acaactggcg cagtaaacag caaaatcagc
     2161 agacgtcttc tgaacgtgaa ctggagatgt ctaccgagat tgcacgtctc aaacgccagc
     2221 tggcagaacg ggatgaagag ctggctatcc tccaaaaggc cgcgacatac ttcgcgaagc
     2281 gcctgaaatg aagtatgtct ttattgaaaa acatcaggct gagttcagca tcaaagcaat
     2341 gtgccgcgtg ctccgggtgg cccgcagcgg ctggtatacg tggtgtcagc ggcggacaag
     2401 gataagcacg cgtcagcagt tccgccaaca ctgcgacagc gttgtcctcg cggcttttac
     2461 ccggtcaaaa cagcgttacg gtgccccacg cctgacggat gaactgcgtg ctcagggtta
     2521 cccctttaac gtaaaaaccg tggcggcaag cctgcgccgt cagggactga gggcaaaggc
     2581 ctcccggaag ttcagcccgg tcagctaccg cgcacacggc ctgcctgtgt cagaaaatct
     2641 gttggagcag gatttttacg ccagtggccc gaaccagaag tgggcaggag acatcacgta
     2701 cttacgtaca gatgaaggct ggctgtatct ggcagtggtc attgacctgt ggtcacgtgc
     2761 cgttattggc tggtcaatgt cgccacgcat gacggcgcaa ctggcctgcg atgccctgca
     2821 gatggcgctg tggcggcgta agaggccccg gaacgttatc gttcacacgg accgtggagg
     2881 ccagtactgt tcagcagatt atcaggcgca actgaagcgg cataatctgc gtggaagtat
     2941 gagcgcaaaa ggttgctgct acgataatgc ctgcgtggaa agcttctttc attcgctgaa
     3001 agtggaatgt atccatggag aacactttat cagccgggaa ataatgcggg caacggtgtt
     3061 taattatatc gaatgtgatt acaatcggtg gcggcggcac agttggtgtg gcggcctcag
     3121 tccggaacaa tttgaaaaca agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
     3181 atcactactc atactataag agggtccact tccccccgga tatatgatga tggatggagt
     3241 atccggagcc cagcatgctc cattagcatc aatatatgtt cccatatctt ttccacgtag
     3301 atctattaca ttagtagaac caggtaatcc attaagattc atccctcctt tcacggaatc
     3361 aagcatgtta tctgatattt ctctcatttt aaaaacctcc tgctcattta tgttcagata
//
LOCUS       DAZUCC010000335         3380 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZUCC010000335 DAZUCC010000000
VERSION     DAZUCC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.337"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..354
                     /gene="radC"
                     /locus_tag="ACHY09_004906"
     CDS             <1..354
                     /gene="radC"
                     /locus_tag="ACHY09_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS3887738.1"
                     /translation="AAREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTE
                     VHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLI
                     VGGSQVFSFAEHGLL"
     gene            423..644
                     /locus_tag="ACHY09_004907"
     CDS             423..644
                     /locus_tag="ACHY09_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS3887739.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAGAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            720..1073
                     /locus_tag="ACHY09_004908"
     CDS             720..1073
                     /locus_tag="ACHY09_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFS3887740.1"
                     /translation="MSDTLPDGNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAG
                     IRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEADTLGSC
                     GYVYMAVYPTPETKK"
     gene            1163..1537
                     /locus_tag="ACHY09_004909"
     CDS             1163..1537
                     /locus_tag="ACHY09_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFS3887741.1"
                     /translation="MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            1534..2022
                     /locus_tag="ACHY09_004910"
     CDS             1534..2022
                     /locus_tag="ACHY09_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS3887742.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGCLPGFPTF
                     DW"
     gene            2034..2231
                     /locus_tag="ACHY09_004911"
     CDS             2034..2231
                     /locus_tag="ACHY09_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFS3887743.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            2316..3158
                     /locus_tag="ACHY09_004912"
     CDS             2316..3158
                     /locus_tag="ACHY09_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS3887744.1"
                     /translation="MSDITISRPELVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITCNLDREIWRDLMQCS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEENILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDQWGLHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIRSLQGKECYEDEMFIIKYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
BASE COUNT          864 a          882 c          847 g          787 t
ORIGIN      
        1 gcggcacggg aatggctgat tctgaacatg gcgggactgg agcgtgaaga attccgggtg
       61 ctgtatctga acaaccagaa tcagttgatt gccggtgaaa ccctcttcac cggcaccatc
      121 aaccgcacgg aagtccatcc ccgggaagtg attaaacgcg ccctgtacca caatgccgct
      181 gccgtggtac tggcacacaa tcacccgtcc ggtgaagtca cacccagtaa ggcagaccgg
      241 cttattacgg aacgtctggt acaggcactg ggcctggtgg atatccgggt gccggaccat
      301 ctgatagtcg gtggcagcca ggttttctcc tttgccgaac acggtctgct ttaacccgtc
      361 accgtcacaa ccacatcata cctgttttca cttttatctt ctgtcttcag aggtatcaca
      421 tcatgaaaat catcacccgt ggtgaagcca tgcgtattca ccaacagcat ccggcctccc
      481 gtctttttcc gttctgcacc ggtaagtacc gctggcacgg cagtgccggg gcgtataccg
      541 gtcgtgaagt acaggatatt cccggtgtgc tggccgtgtt tgctgaacgc cgtaaggaca
      601 gttttggccc gtatgtccgg ctgatgagcg tcaccctgaa ctgagtcagg gcattctgat
      661 gagcagaatt atcgccacca ccggaccatt cttaaccgat tttctgtgag gattttatcg
      721 tgtcagacac acttcccgac ggcaatcacg accgcccctg gtgggggctg ccctgcaccg
      781 tgacgccctg tttcggcgcc cgtctggtgc aggagggtaa ccggttgcat taccttgccg
      841 accgcgctgg tatcagaggc ctgttcagcg atgcagatgc gtaccacctg gaccaggcct
      901 ttccgctgct gatgaaacaa ctggaactca tgctcaccag cggtgaactg aatccccgcc
      961 atcagcatac cgtcacgctg tatgccagag ggctgacctg cgaagccgac accctcggca
     1021 gttgtggcta cgtttatatg gctgtttatc cgacgcccga aacgaaaaag taactctcca
     1081 gaataaccct ctgctccggc ctggtgtttt caccacacca ctttcccatt tttcatcttt
     1141 gcatatcagg aaaatcttca gtatgaaaac attacctgta ttacccgggc agtcggccag
     1201 ttcacgccca tctcctgttg aaatctggca gacactgctc acccgactgc tggaccagca
     1261 ctatggcctt acgctgaatg acacaccgtt cgctgatgaa cgtgtgattg agcagcatat
     1321 tgaggcaggc atttcactgt gtgatgcggt gaattttctc gtggaaaaat acgcgctggt
     1381 gcgtaccgac cagccgggat tcagcgcagg aacttcgtcg cagttaatca acagcattga
     1441 tattctccgg gcacgccggg caacaggtct gatgacccgc gacaattaca gaacggtaaa
     1501 taacattacc ctgggtaagc atccggagaa acgatgaaac tttccctgat gctggaagcc
     1561 gacagaatta atgtgcaggc actgaacatg gggcaaattg tcgttgacgt cgatggtgtt
     1621 aatctcactg aactgattaa caaggtcgct gaaaacggtt attcactccg cgtggtggag
     1681 gaatccgacc aacagtcaac ctgcacacta ccaccgtttg caacccttgc cggcatacgc
     1741 tgcagtaccg cacatatcac ggaaaaggat aacgcctggc tgtactcgct gtcgcatcag
     1801 accagtgacg ttggtgaatc agaatggatt catttcacag gtagcggata tctgttacgt
     1861 accgatgcgt ggtcataccc ggttctgcgg cttaaacgcc tggggctgtc aaaaacgttc
     1921 cgccgtctgg ttgtcacact catccgacgt tatggcgtca gtctcattca tctggatgcc
     1981 agtgccggat gtctgccggg tttccccact ttcgactggt aaccagaaac aacatgaaat
     2041 cattaaccac ggaaacagcg ctggatattc tgattgcatg gctgcaggac aatatcgact
     2101 gcgaatcggg aattatcttt gacaacgatg aggataaaac ggattcggca gcactgctgc
     2161 cctgcattga acaggccagg gaagatatcc gtaccctgcg ccaactgcag tttctgcaac
     2221 agaaccggtg agtcttactc accagacttc attaccctga cgtcagcctg aacagggctg
     2281 gcgttttcgt ttataaaccc aaagggaaac cgataatgtc agacatcaca atctcccgtc
     2341 cggaactggt caccgggcat acggacgtta tctgctcaac cagtatccgc cacattctgg
     2401 ctgtacgaaa gagtacactg ctgcaaatcg acacacttat ccggcaactg gctgaaatat
     2461 cagcaatgac agaaagtatt ggcggtaaaa ccgcactgga ctgggcaatg aaacaggatt
     2521 ttcgctgcgg ttgctggctg atggaaaaac ctgaaacggc aatgaaagcc atcacctgca
     2581 atctcgatcg agaaatctgg cgtgacctga tgcaatgctc cgggatgctt tccttaatgg
     2641 atgcacaggc ccgtgataca tggtaccggt cactggagta cgataatttt ccggaaatca
     2701 gtgaagagaa tattctgagt acgtttgagc agctacacca gaataaggat gaagtgtttg
     2761 agcgaggagt gatcaacgtc ttcagagggt tgagctggaa ttacaaaacc aattgcccct
     2821 gtaaatttgg caataaaatt atcgtcaaca atctggtgag gtgggaccag tggggattgc
     2881 atcttatcac cgggcaacag gcagatagac ttgccgacct ggaaagaatg ctgcatctgt
     2941 tcagcggcaa accgatcccc gacaacaggg aaaacataac tattcatctg gacgatcaca
     3001 tccggtctct tcagggaaaa gaatgctatg aagatgagat gttcattatt aaatacttta
     3061 agaagggctc tgcgcacatc acgttcagga agccagaact ggttgacaga ctgaatgata
     3121 ttatcgcaaa acactatcct gacatgctgg cagtatagct ttgccagtat gctcaaagat
     3181 cggaaattgt ccgataagat atgatcgtgg ggcatataaa ccggtttcag atggcatacc
     3241 cggtggcata ctgtctggaa ggataaataa aaaagcgcat gttaaacaat aacatgcgct
     3301 tgtaatctgg cggtgagggg gggattcgaa cccccgatac gttgccgtat acacactttc
     3361 caggcgtgct ccttcagcca
//
LOCUS       DAZUCC010000336         3365 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZUCC010000336 DAZUCC010000000
VERSION     DAZUCC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.338"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(46..342)
                     /gene="yciI"
                     /locus_tag="ACHY09_004913"
     CDS             complement(46..342)
                     /gene="yciI"
                     /locus_tag="ACHY09_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFS3887745.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            566..1285
                     /gene="tonB"
                     /locus_tag="ACHY09_004914"
     CDS             566..1285
                     /gene="tonB"
                     /locus_tag="ACHY09_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFS3887746.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(1325..1723)
                     /gene="yciA"
                     /locus_tag="ACHY09_004915"
     CDS             complement(1325..1723)
                     /gene="yciA"
                     /locus_tag="ACHY09_004915"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFS3887747.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1828..2367)
                     /gene="yciB"
                     /locus_tag="ACHY09_004916"
     CDS             complement(1828..2367)
                     /gene="yciB"
                     /locus_tag="ACHY09_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFS3887748.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2397..3140)
                     /gene="yciC"
                     /locus_tag="ACHY09_004917"
     CDS             complement(2397..3140)
                     /gene="yciC"
                     /locus_tag="ACHY09_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFS3887749.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(3168..3257)
                     /locus_tag="ACHY09_004918"
                     /pseudo
     CDS             complement(3168..3257)
                     /locus_tag="ACHY09_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT          965 a          834 c          802 g          764 t
ORIGIN      
        1 agaacacggt tgctaaaacc gtgcccttaa atattgaatt tctattcaga acactttctt
       61 aaatggtttc actgaaacgt gttcatagac tcctgccgct acgtacgggt cagcatcggc
      121 ccaggcctga gctgcttcca gcgattcaaa ttcagcaata acggttgagc cagtaaatcc
      181 cgcagcccct ggatcgttac tgtctactgc tggcattgga ccggcggtca acaatcgacc
      241 ttcatcatgc agtaactgta aacgtgctaa atgtgccgga cgaacggaaa ggcgtttttc
      301 gaggctatca gctttgtctt gagcgtaaat aacatacaac acgggcacaa ctccttgttc
      361 gggaaagttg taaagtacgt tatttgaaag ggcgaagatc tgcaacggaa agatgatgtc
      421 tttgttaaga ccatttaaaa agtaaggtca attacgccaa aaatgacatt ttcactgatc
      481 ctgatcgtct tgccttattg aatatgattg ctatttgcat ttaaaatcga ggcctggttt
      541 ttctactgaa atgattatga cttcaatgac ccttgattta cctcgccgct tcccctggcc
      601 gacgttactt tcggtctgca ttcatggtgc tgttgtggcg ggtctgctct atacctcggt
      661 acatcaggtt attgaactac ctgcgcctgc gcagccaatt tctgtcacga tggttgcgcc
      721 tgcggatctc gaaccgccac aagccgttca gccgccaccg gagccggtgg tagagccaga
      781 accggaacct gagccgatcc ccgaaccgcc aaaagaagca ccggtggtca ttgaaaagcc
      841 gaagccgaaa cctaagccaa aaccgaagcc ggtgaaaaag gtacaggagc agccaaaacg
      901 cgatgtcaaa cccgtagagt cgcgtccggc atcaccgttt gaaaatactg cacccgcgcg
      961 ccctacatca agtacggcaa cggctgcaac cagcaagccg gttaccagtg tggcttcagg
     1021 accacgcgca ttaagccgca atcagccgca gtatccggca cgagcacagg cattgcgcat
     1081 tgaagggcag gttaaagtta aatttgatgt cacgccggat ggtcgcgtgg ataacgtaca
     1141 aatcctctca gccaagcctg cgaacatgtt tgagcgtgag gtgaaaaatg cgatgcgcag
     1201 atggcgttat gagccgggta agccaggcag tgggattgtg gtgaacatcc tgtttaaaat
     1261 taacggcacc accgaaattc agtaagcaga aagtcaaaag cctccgaccg gaggcttttg
     1321 attattactc aacaggtaag gcgcgaggtt ttccttcagg atcaaccgcg acatacttaa
     1381 ataatgcttc tgtcgcttta tagcgttgcc cgatgggttc agacgcgact tttttcaccc
     1441 acacttcaat attaatgcta accgatgtcg tccctttctg gacacagcgt gcatagcagc
     1501 acaccacatc gccgaccgca accggtcgta agaaagtcat tccttcaacc cgcacggtca
     1561 ctacgcgacc gtgggcaatt tctttcgcca gaatagcgcc gccaatatcc atttgtgaca
     1621 ttaaccaacc accaaagatg tcaccattgg cattggtatc ggcgggcatg gctaaagtac
     1681 gtaaaacaag atcgccctga gggacgttat gtgttgtaga catggtaaaa ccgacttaaa
     1741 aggaaaatca caggcgcgat gctactatga ttttcctgag gagaacagag gagatacagc
     1801 gccagccccg aaggactggc agtctggtta ggatttatct tcctgcggca tgtggcggta
     1861 gatatagata ccgcttaaca atgtgaagat taaggtaagg gcggtcaggc caaagacttt
     1921 aaagttgacc caaatatttt gcggcagcca gaatgcgatg tagatgtttg ccagaccgca
     1981 aaggataaag aaaacagccc aggccagatt cagcttcgac cataccggtt gcggcagcgt
     2041 gagttcttta cccagcatcc gctgaattag cggctttttc atcacccatt ggctgactaa
     2101 cagggcaccc gcaaacaggg cataaatgac agtaaccttc catttaataa actcatcatt
     2161 gtggaagaac agggtcaagc caccgaagac gaccaccaga acaaaagtga tcagggccat
     2221 cttctcaacc ttacgaaagc gaacccagct atatataagc acaatcgccg tggcgacgat
     2281 cagcgccgca gtagccgcat agatgtcata aatcttgtaa aacgcgaaaa agacaaccag
     2341 cggtaaaaaa tcaagaaact gcttcatttt acgattccgt aatcaaatgc ttaaggttat
     2401 tggcgaatca acatatacag gcggaacaga tagatgagca gtacggctga aatcaggttg
     2461 ctcaaggtgt tcgccagtac ggcaccaatt tccggggtta atgcggcaaa agaagaggca
     2521 aaaagcagca acagtgtctt tgccagcaac cagctcagta ctgcgggtgc caccagacgc
     2581 atattcgccc aagtcagccg catactgcta cgcatcgagg caaaaacgcc cattttgtcc
     2641 tgtaccaaca tcaccggtgc cagggccagt aaaatggcca tgataattcc cggaacgacc
     2701 accagcatga tgccaatctg tactaaaagg gtagtcagaa aaatcagaat aaataacttt
     2761 ggcaatatcg gcgcactggc accaatagct cgcagcgcac tgactctctg acccgcagac
     2821 accagctgga taattaatat tacgcctccg gcgagaatgg cgttaccgat taatcctgaa
     2881 aaagtggacg ccgctgaagc ctgcagcaaa atttgttgct gttccggtga catattctga
     2941 accaggtcga acaacccact actgccgcta acgggcacgc cgtcattgag ctgcgcaagc
     3001 tgtgcatcac tgggtgagaa aacatgccct aacaccactg tgataaacgc acatagcaac
     3061 gataccaaca gaatggtcat aaattgatta cggaagaaat ttccggtgtc acggtatacg
     3121 gactgcgccg tgatagacat gcactctcct tgagttttat gcaggtgtta attagcgggt
     3181 aattgtaccc tggttaagcc tcgggtggca gcatcaggtc ttgtatggaa aagcatatct
     3241 ttgtaaagcg gaggtaattg ctggccttca gacgtgggca aatcgcggca agtggcgatc
     3301 gagttaatat ttgcgtagcg aaaatattta aaaattgatt taaatcacat taaccaggat
     3361 tctca
//
LOCUS       DAZUCC010000337         3336 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZUCC010000337 DAZUCC010000000
VERSION     DAZUCC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.339"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            138..1103
                     /gene="dusB"
                     /locus_tag="ACHY09_004919"
     CDS             138..1103
                     /gene="dusB"
                     /locus_tag="ACHY09_004919"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS3887750.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1129..1425
                     /gene="fis"
                     /locus_tag="ACHY09_004920"
     CDS             1129..1425
                     /gene="fis"
                     /locus_tag="ACHY09_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFS3887751.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1511..2395
                     /gene="yhdJ"
                     /locus_tag="ACHY09_004921"
     CDS             1511..2395
                     /gene="yhdJ"
                     /locus_tag="ACHY09_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFS3887752.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAVLVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVANGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2479..2658
                     /locus_tag="ACHY09_004922"
     CDS             2479..2658
                     /locus_tag="ACHY09_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFS3887753.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2661..3323)
                     /gene="acrS"
                     /locus_tag="ACHY09_004923"
     CDS             complement(2661..3323)
                     /gene="acrS"
                     /locus_tag="ACHY09_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFS3887754.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT          908 a          782 c          791 g          855 t
ORIGIN      
        1 attatcgcgc cacgctcatt ttaaatgcaa ttctttgatc catctcagag gattggtcaa
       61 agtttggcct ttcatctcgt gcaaaaaatg cgtaatatac gccgccttgc agtcacagta
      121 tggtcatttc ttaactcatg cgcatcggac aatatcagct cagaaatcgc ctgatcgcag
      181 cgcccatggc tggcattaca gacagacctt ttcggacgtt gtgctacgag atgggagccg
      241 gattgacagt atccgagatg atgtcttcta accctcaggt ttgggaaagc gacaaatctc
      301 gtttacggat ggtgcacatt gatgaacccg gtattcgcac cgtgcaaatt gctggtagcg
      361 atccgaaaga aatggcagat gcagcacgta ttaacgtgga aagcggtgcc cagattattg
      421 atatcaatat gggttgcccg gctaaaaaag tgaatcgcaa gctcgcaggt tcagccctct
      481 tgcagtaccc ggatgtcgtt aaatcgatcc ttaccgaggt cgtcaatgca gtggacgttc
      541 ctgttaccct gaaaattcgc actggctggg caccggaaca ccgtaactgc gaagagattg
      601 cccaactggc tgaagactgt ggcattcagg ctctgaccat tcatggccgt acacgcgcct
      661 gtttgttcaa tggagaagct gagtacgaca gtattcgggc agttaagcag aaagtttcca
      721 ttccggttat cgcgaatggc gacattactg acccgcttaa agccagagct gtgctcgact
      781 atacaggggc ggatgccctg atgataggcc gcgcagctca gggaagaccc tggatctttc
      841 gggaaatcca gcattatctg gacactgggg agttgctgcc cccgctgcct ttggcagagg
      901 ttaagcgctt gctttgcgcg cacgttcggg aactgcatga cttttatggt ccggcaaaag
      961 ggtaccgaat tgcacgtaaa cacgtttcct ggtatctcca ggaacacgct ccaaatgacc
     1021 agtttcggcg cacattcaac gctattgagg atgccagcga acagctggag gcgttggagg
     1081 catacttcga aaattttgcg taaacagaaa taaagagctg acagaactat gttcgaacaa
     1141 cgcgtaaatt ctgacgtact gaccgtttct accgttaact ctcaggatca ggtaacccaa
     1201 aaacccctgc gtgactcggt taaacaggca ctgaagaact attttgctca actgaatggt
     1261 caggatgtga atgacctcta tgagctggta ctggctgaag tagaacagcc cctgttggac
     1321 atggtgatgc aatacacccg tggtaaccag acccgtgctg cgctgatgat gggcatcaac
     1381 cgtggtacgc tgcgtaaaaa attgaaaaaa tacggcatga actaattcag gttagctaaa
     1441 tgcttgatta aaaaggcgct actcggcatg gggaagcgcc ttttttatag gtgtcacaaa
     1501 gggagtgacc atgagaacag gatgtgaacc gacccgattt ggtaatgaag caaagaccat
     1561 catccagggt gatgcattaa ccgaacttaa aaagctcccc gccgaaagcg tcgatctgat
     1621 ctttgccgac ccaccgtata acatcggtaa aaattttgat ggtctgatcg aagcctggaa
     1681 agaagatctg tttatcgact ggctgtttga agtgattgca gagtgccacc gcgttctgaa
     1741 aaagcagggc agcatgtaca tcatgaacag cacggaaaac atgcccttta tcgatctcca
     1801 gtgccgcaag ctttttacca tcaaaagtcg catcgtctgg tcatatgaca gttcaggagt
     1861 gcaggcgaaa aaacactacg gctccatgta cgaacccatc ctgatgatgg tgaaagacgc
     1921 aaagaactac acattcaacg gtgatgctgt tctggtagaa gccaaaaccg gatcgcagcg
     1981 cgcgttgatc gattatcgca aaaatcctcc acagccttac aatcatcaaa aagtaccggg
     2041 taacgtctgg gattttcctc gcgtgcgtta tttgatggat gaatatgaaa accacccgac
     2101 gcaaaaaccg gaagccttac tgaaacgcat tattctcgcc tcttccaacc caggcgatat
     2161 cgttctcgac ccgtttgccg gtagctttac taccggtgcc gtagccgtcg ccaacgggcg
     2221 aaaattcatt ggtattgaga tcaacagcga gtacatcaaa atggggcttc gacggctgga
     2281 tgtcgcgtcg cattactctg cggaagaact ggcgaaagtg aaaaaaagaa agaccggcaa
     2341 cctgtcaaaa cgaagccggt taagcgaagt tgaccccgat ctcattgcaa agtaaaggaa
     2401 gtgtaagcct ggttttcaga ttattcattt cgtgtatatt tcctgccaga cttggttaaa
     2461 catgcacagg ctcaggtaat gattcgcaag tattggtggc tcgtcgtttt cgctgtcttc
     2521 gtttttctgt ttgatacttt actgatgcag tggattgaac tgctggcaac agaaacagac
     2581 aaatgccgca atatgaactc agttaatcca ctaaaactgg ttaactgtga cgaactgaat
     2641 tttcaggaca gaatgtgaat ttatatgaca cttaattcat tcgttttatg aattaatttc
     2701 gttatgtttt catctggcat gaacattctt aatacgttat cgaccagagc gggggcttgt
     2761 ttataaagat cataacccgc catattcatt aaccagtttt gaacaattcc gctgaaggca
     2821 ccatcaataa taatcatcac aacatctaaa tcgagattat ttgctacaca accttgttgc
     2881 tgacacgcct gcaatacttc gcggagagtc tgcggattaa agcccatctt ttcgcgtatc
     2941 actccctcgg ccagcatctc atcattaaat tcacatttgt gatataagat tttcagcaac
     3001 gcctgctggc ggggaatttt ggcaatatat tgcaaaccga caatcaattt ttcacgcaat
     3061 tgctgaaacg ggtcatgctc taacccagcg gtcaagtgat cctggattaa ctcccgcaat
     3121 gaaggctgtt gcaaccacat ctcattaaac agttgggtct tgttctcgaa gtgccagtag
     3181 atagcgccac gcgtaacgtt agcagcgtcg gcaatgtcgt tgagcgtcgt cttgcttacg
     3241 ccatgttgcg caaactgggc gatggcagtt tcaatcagtt cttgccgggt cttcagagct
     3301 tcggctttgg ttctttttgc catgattaat tattca
//
LOCUS       DAZUCC010000338         3317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZUCC010000338 DAZUCC010000000
VERSION     DAZUCC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.340"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..568)
                     /locus_tag="ACHY09_004924"
     CDS             complement(<1..568)
                     /locus_tag="ACHY09_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS3887755.1"
                     /translation="MNSTDLIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TTEILATLPSLKKMGNYLISFTRSHRSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPG"
     gene            complement(571..1746)
                     /locus_tag="ACHY09_004925"
     CDS             complement(571..1746)
                     /locus_tag="ACHY09_004925"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS3887756.1"
                     /translation="MDIFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYHQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDVIKHSERNILTASLIIKNEGGYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELLKIATSCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYLISTDEDYNHKFRTISRYRDFVNSPSVLNIIATI
                     VAYNECEGWLKSLKAHIASNIQFTQQYLNENTPELIVRPADGCYFSWIDCSAIGYSFD
                     EFYSRLIHEGKVGIMAGNVYGTEGEGYLRLNLACGREKLYMGLTRLASVIKNINQGK"
     gene            complement(1746..>3317)
                     /locus_tag="ACHY09_004926"
     CDS             complement(1746..>3317)
                     /locus_tag="ACHY09_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS3887757.1"
                     /translation="KKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAKLIP
                     FLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGFMAM
                     HLGTNFYLKQHDLLVVADQMSTHGQNIILGIQSYNTSVLGGIVAGLLVASMYKKIVNL
                     RIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVGYFF
                     YAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLDL
                     AGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIEFLF
                     LFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAVCVE
                     VAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADTPTLDKSELQAAEQM
                     VKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVIIGTK
                     VIALRRDMDNYMGMR"
BASE COUNT         1025 a          766 c          623 g          903 t
ORIGIN      
        1 ctcctggatg atataagccg aaatcagcac gagtaaactt tttcaattcg gagagcgcaa
       61 gcgccaccgc atcaccaaca accagaacga ctgttgatga gcatgacggc gctaaaccta
      121 aattatccgc ctcacttttt acgggaattt caacggataa atcacaactt atcgcaagcg
      181 atgagcgatg actgcgggta aaggagatca aataattacc catttttttc agactaggta
      241 gtgttgccag gatttctgtc gtctcaccac tgtttgaaat aagaataaca acatcatctt
      301 tcgccaccat cccaagatcg ccgtgtacgg cttcagtacc gtgaacaaaa aacgagggag
      361 ttccagtgct ggcaaatgtt gcggcgagtt ttcgggcaat aataccggac ttaccgacac
      421 caataaaaac aactttcccc tgacaagact gtaatacttt aattaaattt gcgacctccg
      481 aaagggggga atcaataact tcttcaactg ctttatgttc gttacgcata aaatgtttaa
      541 tgagatggat aagatccgta ctgttcattt ttattttcct tgattaatat ttttaattac
      601 actcgcaaga cgcgtaagcc ccatatataa tttttctcga ccgcaggcca ggttcagacg
      661 aaggtagccc tccccctccg ttccatagac attccccgcc ataatgccca ctttgccttc
      721 atgaattaat cgactgtaga attcatcaaa ggaataacca atagcagagc aatctatcca
      781 cgaaaaatag caaccatcag ctggccttac gatcaattct ggagtatttt cgttcagata
      841 ctgctgggtg aattgaatgt tagaggcgat atgtgccttt aatgacttta accatccctc
      901 acattcatta taggcaacaa tggttgcaat tatattcagt actgatgggg agtttacgaa
      961 atcgcgatac ctcgatatcg ttctgaactt atgattataa tcctcgtcag ttgaaatcag
     1021 atacgatcct gttagcgcag gaacattaaa tgttttcgat atcgagttaa ataccactac
     1081 tttttctttt tttattttaa gcgcggatgt aaagctacga ttaaaaacaa aatcactatg
     1141 aatttcatct gaacataacc agacattatg ccttgtacag cttgtagcga ttttaagcag
     1201 ttcttcttcc gaccagactg ttcctgtcgg attatgagga ttacataata acaccatctt
     1261 tgcgctttta atcttaagat caaaatcttg ccagttgata gcgtagcccc cctcattttt
     1321 tataatcaaa gaggctgtca gaatatttcg ctcgctatgt ttaattacat caaaaaatgc
     1381 attatagaca ggtgtaaaaa cgagaatatt atcaccatgc gatgtcaata actctattgc
     1441 ctttgcaata gagtacatca cactggggct ataggttatc cattcaggtt taatgtctgt
     1501 ttgatattgt tgatgatacc agtttactat cgcattcaga tattcaccat gttgccaacg
     1561 actgtaccct aaaatcgggt gttccaggcg tttttttaat gctctggtga tagcgtctgg
     1621 tatcggaaga tccatatctg aaatagagaa gggtaataca tcttttacac caaatctgtc
     1681 ctcgcaaaag tcccattggg tgcaatatgt tcctttacgg gaaataggag tattaaaaat
     1741 atccatcagc gcatccccat gtagttatcc atatccctac gcagggcgat gactttagtg
     1801 ccgataatga cttgtagggt attttgatca agtttaacaa ccgcaatccc gccagctctt
     1861 tttattgcaa cttcatcaat caatcccata tctgcgattg ttaaacgtaa acgagttacg
     1921 caattatcca gtgacttaat attctcttta ccaccaagtg ccttaaccat ctgctccgct
     1981 gcttgcaact ctgatttatc aagagttggg gtatccgcta tagccgtatc ttcgtgagcg
     2041 ttcagcgtga caggactgga ctcttcacgt cctacggtca tcaggttaaa tttgagaatg
     2101 gtccaacgga acacaaagta ataaatgaag aaataggcca cttcaacaca caccgcgatc
     2161 ggccaacctg ttcttgtgcc acgcaatacg ccaaagctaa taaattgaat cagatcgcca
     2221 gtaaatccca tcttcacgcc catatatggc aagaccatgt tggcgaatcc attcataaag
     2281 gcatggaaga caaacagcag cggactgaca aataagaaca gaaattcaat cggttcgctg
     2341 acgccaccaa taataaccgt aagcacaccg gaaataagca atgctttgat agcctttctg
     2401 ttttgagcat gagcagtgcg atacatggcc agtgcagcac cagccagacc gtactgaatc
     2461 atcagtttgc cctgctccat tttccccgcc aaatccagcg gaatgaccgc attctctttg
     2521 acgtacgcca taaacatgtt cagggtgccg taatactctt cgccgccaat cacagccgaa
     2581 ccgccgattg gcgtaaagcg gaacactgac gtcaccagat ggtttaagcc aaaaggaatc
     2641 gtcacacgtt cggcaactgc atagaagaaa taaccaacag gaccggaagt tgatatccag
     2701 tggccaatga gcatgaagag attgaaaaat ggcggccaga taaaaggaat gatcagacca
     2761 aatccactca tcacaatcag tgtaatgata ggcaccagac gtgggccgct ataaaaacct
     2821 aatgattctg gaatgcgtaa attaacgatc tttttataca tgctggcgac taataaccca
     2881 gcaacaattc cccctaacac gctggtattg taggactgga tccccagaat gatgttttgc
     2941 ccatgtgtcg acatttggtc agcaacgacc aataagtcgt gctgtttaag ataaaagttc
     3001 gttcccagat gcatcgccat aaaaccaatt aagccagaaa aagcaccata ggctttatcc
     3061 tctttatctt ttaataatcc taagggaatc gctatcgcaa acaatacagg taaattaaca
     3121 aaggcaaaca aaccaagact aacaatgaaa tcaagtatgg ttttaattat tggaatagcc
     3181 agaaatggaa ttaactttgc catatcatca ctggctaaac cacttcccag ccctagcatc
     3241 atgccacata cacttagcag agcaatggga tacataaatg ccttccccag gctctgaaaa
     3301 aaactccagg ctttctt
//
LOCUS       DAZUCC010000339         3297 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZUCC010000339 DAZUCC010000000
VERSION     DAZUCC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.341"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..354
                     /gene="radC"
                     /locus_tag="ACHY09_004927"
     CDS             <1..354
                     /gene="radC"
                     /locus_tag="ACHY09_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS3887758.1"
                     /translation="AAREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTE
                     VHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLI
                     VGGNQVFSFAEHGLL"
     gene            417..638
                     /locus_tag="ACHY09_004928"
     CDS             417..638
                     /locus_tag="ACHY09_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS3887759.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            801..1175
                     /locus_tag="ACHY09_004929"
     CDS             801..1175
                     /locus_tag="ACHY09_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFS3887760.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRNQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPAQAPATTS"
     gene            1222..1599
                     /locus_tag="ACHY09_004930"
     CDS             1222..1599
                     /locus_tag="ACHY09_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFS3887761.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            1596..2084
                     /locus_tag="ACHY09_004931"
     CDS             1596..2084
                     /locus_tag="ACHY09_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS3887762.1"
                     /translation="MKLTLTLEADNVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITETDNAWLYSLSHQTNDDCETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            2096..2293
                     /locus_tag="ACHY09_004932"
     CDS             2096..2293
                     /locus_tag="ACHY09_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFS3887763.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQVQLLHQNR"
     gene            2378..3223
                     /locus_tag="ACHY09_004933"
     CDS             2378..3223
                     /locus_tag="ACHY09_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS3887764.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDWWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKECYEDEMFSIRYFKKGSAHITFKNQELVDRLNDIIARHYPR
                     MLPSL"
BASE COUNT          829 a          888 c          816 g          764 t
ORIGIN      
        1 gcggcacggg aatggctgat tctgaacatg gcgggactgg agcgtgaaga attccgggtg
       61 ctgtatctga ataaccagaa tcagttgatt gccggtgaaa ccctcttcac cggcaccatc
      121 aaccgcacgg aagtccatcc ccgggaagtg attaaacgcg ccctgtacca caatgccgct
      181 gccgtggtgc tggcgcacaa tcacccgtcc ggtgaagtca cacccagcaa ggcagaccgc
      241 cttatcacgg aacgtctggt acaggcactg ggcctggtgg atatccgggt gccggaccat
      301 ctgatagtcg gtggcaacca ggttttctcc tttgccgaac atggtctgct ttaacccgtc
      361 acaaccacat cacgcttgtt ttcactttta tcttctgtct tcagaggtat cacactatga
      421 aaatcatcac ccgtggtgaa gccatgcgta ttcaccaaca acatccggca tcccgtcttt
      481 ttcctttctg taccggcaaa taccgctggc acggcagcac tgaagcgtat accggtcgtg
      541 aagtgcagga tattcccggt gtgctggccg tgtttgctga acgccataag gacagttttg
      601 gcccgtatgt ccggctgatg agcgtcaccc tgaactgatg ttccgtcacc cggaaaacga
      661 gtacgaccag atggactcag acgccgctgt ggatgaggcc ctgctcatca acgaatacac
      721 ctttaccgaa gcaggtcagc gcgccggctg acctgtcatc gttaaaccat tcttaaccac
      781 ttttcagaga ggattttatc gtgtcagaca aactctccgg gataactcat cccgacgaca
      841 atcacgaccg cccctggtgg gggcttccct gcacagtgag gccctgtttt ggtgcccgtc
      901 tggtgcagga gggtaaccgc ctgcattacc ttgccgaccg cgccggtatc agaggccgct
      961 tcagcgacgc agatgcatac catctggacc aggcctttcc gctgctgatg aaacaactgg
     1021 agctcatgct caccagcggt gaactgaatc cccgaaatca gcataccgtc acgctgtatg
     1081 caaaagggct gacctgcgaa gccgacaccc tcggctcctg tggttacgtt tatctggctg
     1141 tttatccggc acaggcaccc gcaaccacct cataaccccg aagctctcac ctttagcact
     1201 accagttaca gagaacttaa gatgaaaaca ttatccgaca cacatgtacg ggaggtatct
     1261 cgctgcccgt ctcccgtcac catctggcag acactgctca tccgactgct ggaccagcat
     1321 tatggcctca cactgaatga cacaccgttt gccgatgaac gtgtgattga gcagcatatt
     1381 gaagctggca tttcactgtg cgatgcagtg aactttcttg ttgaaaaata cgcactggtg
     1441 cgtaccgacc agccggaatt cagtgcctgt acccgttctc agttaataaa cagcatcgat
     1501 atcctccggg ctcgcagggc gaccggcctg atgacccgcg acaattacag aacggtaaat
     1561 aacattaccc aggggaagca tccggaggcg aaacaatgaa actgactctg acgctggaag
     1621 cagacaacgt taacgtgcag gcgctgaaca tgggacgaat tgtcgttgac gtcgatggtg
     1681 ttaatctcgc tgaactgatt aacgtggtct gcgacaatgg ccactccctt cgtgttgttg
     1741 atgaatctga ccgggtctca acagactgca cgccaccatt tgctgccctt accggcatac
     1801 gctgcagtac cgcacatatc acggaaacgg acaacgcctg gctgtactcg ctgtcacacc
     1861 agactaatga cgactgcgaa acagaatgga ttcattttac aggcagcgga tatctgttac
     1921 gtaccgatgc gtggtcatac ccggttctgc ggcttaagcg cctgggcctg tcaaaaacgt
     1981 tccgtcgtct ggttgtcaca cttacccgac gttatggcgt cagtctcatt catctggatg
     2041 ccagcgctga atgcctgccg ggtttaccca ctttcaactg gtaaccagga acaacatgaa
     2101 atcattaacc acggaaacag cactggatat tctgattgcg tggctgcagg acaatatcga
     2161 ctgcgaatcg ggaattatct ttgacaacga tgaggataaa acggattcgg cagcactgct
     2221 gccctgcatt gaacaggcga gggaggatat ccataccctg cgccaggtgc agcttctgca
     2281 ccagaaccgg tgagtcttac tcaccagact tcattccact gacgccagcc tgaacagggc
     2341 tggcgttttc atttatctgc gaaaaggaat atcgattatg tctgcaatca cagtctcccg
     2401 tccggaagtg gtcaccgggc atacggacgt tatctgctcc acctcagtca gtcacattct
     2461 ggctgtgcga aagactacac tgctgcaaat cgacacactt atccggcaac tggctgaaat
     2521 ctcggtactg acagaaagta ttggaggtaa aactgctctg gactgggcca tgaaacagga
     2581 ttttcgctgc ggctgctggc tgatggagaa acctgaaacc gcaatgaaag ctatcacacg
     2641 caatctcgat cgtgaaatct ggcgtgacct gatgcaacgt tccgggatgc tttccttaat
     2701 ggatgcacag gcccgtgata catggtaccg gtcactggaa tatgataatt ttccggaaat
     2761 cagcgaggcg aatatcctga gcacatttga acaactgcac cagaataagg atgaggtgtt
     2821 tgagcgaggg gtgatcaacg tcttcagggg actgagctgg aattacaaaa ccaattgccc
     2881 ctgcaaattt ggcagtaaaa ttatcgtcaa caacctggtg aggtgggact ggtgggggtt
     2941 tcatcttatc accgggcaac aggcagatcg acttgccgat ctggaaagaa tgctacacct
     3001 gttcagcggc aaaccgatcc ccgacaaccg ggaaaacatc acccttcgtc tggatgatca
     3061 catccagtct gttcagggta aagagtgcta tgaagatgag atgttcagca tcagatactt
     3121 taagaagggc tccgcgcaca tcacgttcaa aaatcaagaa ctggttgaca ggctgaatga
     3181 cattattgca agacactatc cgagaatgtt accttcatta taaagttcta agaatgaatt
     3241 caattttgca ttttagcttc attccctcac gcaaaattat ttgggggttc ttatggg
//
LOCUS       DAZUCC010000340         3295 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZUCC010000340 DAZUCC010000000
VERSION     DAZUCC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.342"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(58..777)
                     /gene="dcuR"
                     /locus_tag="ACHY09_004934"
     CDS             complement(58..777)
                     /gene="dcuR"
                     /locus_tag="ACHY09_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFS3887765.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(774..2405)
                     /locus_tag="ACHY09_004935"
     CDS             complement(774..2405)
                     /locus_tag="ACHY09_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS3887766.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2585..2815
                     /gene="yjdI"
                     /locus_tag="ACHY09_004936"
     CDS             2585..2815
                     /gene="yjdI"
                     /locus_tag="ACHY09_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFS3887767.1"
                     /translation="MDQALLDRGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2827..3099
                     /locus_tag="ACHY09_004937"
     CDS             2827..3099
                     /locus_tag="ACHY09_004937"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3887768.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT          841 a          775 c          823 g          856 t
ORIGIN      
        1 gccaggaata ttttgcatta cagtaataat tattacttac agataaccag cgatccgtta
       61 ttggcaatat tgtttcagca gtgagtagtg ttctgcctga atacggtaac ggtaaaccgg
      121 acgccctgtg acgccataat ggatactggt gaacaagatg tggcagttga ccagccagat
      181 gaggtattta cggcaggaaa cacgcgagat gttaacctcg ttggccagct cgtcggttga
      241 aaactcatag tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaacg tctgcggcgt
      301 caagcctttt ggcaagcgac gaggatcctg ttcgttggag ctgctgccgt ggattagctg
      361 atcaagctcg gcctggtcat aatactgatg tttttccagc gccattttct tttgccgcca
      421 gccggtgagc gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt aatccacgac
      481 accgtaatgc agcgaatctt taatggttgc cgcatcggct gcggaggaga tgacaatcac
      541 atcacttttg caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt tctctttttg
      601 catatagata tcgagcaata tcaggtcgat aggcgtatcg ctattgaaga taatctcttt
      661 ggctttctcc agcgtcgaag ccgttccaca gcattgaaag cctgggattt gtgccacgta
      721 tcggcgattc agctccgcga ccattgcgtc gtcatcgata attaatacat tgatcatctg
      781 ttcgacctct ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat cccgggttcc
      841 gattccacgg cgatgctgcc gccgagattt tctacctgtt gtttgacaag tgctaaaccg
      901 acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc gattttgtcg
      961 ggtgcgatcc ccggtccatc atcgttaact tcacagtgca gccagccgtg acggtagtgc
     1021 aatgttacgc taatttcgcc accgggttcc ggtcctaatg cctccagcgc gttttctatc
     1081 agatttccca gcgtggtaat cagcgtcgcg acctggtctt cactgccgct gtctggcaac
     1141 tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt aatcttactg
     1201 attaaaaaac cagcgataac cggagatttg atcttaccca gcagagagcc aatctcttct
     1261 tgatagttat tggctgtttt gagaatgtaa tcttccaact gcttataact cttcagatgc
     1321 aataatccga gaatcacgtg caatttattc ataaattcgt gggatcgttc acgaagtgcg
     1381 tcagcatagt tgaccagacc atcgagtcgc tgcatcagtt tacgtacttc agttttgtcc
     1441 ctgaaggttg aaatggcacc gatgataacg ccattactgc gcaccggaac ggtgttgatc
     1501 agtaatagcc ggtctttaat cgtaatctct tcgtcgcgcc gcggggtacc gtcgcgtaac
     1561 acttccgaga catctaccac ctgtgaccac gagtggctta gcgtcgacag cttctcatcg
     1621 tcctgcgact tacggtaatt cagcaattct tgtgcggcat cgttgatcag cgtgacttca
     1681 ccgcgatcgt ccacggcaac gacgccttct ttgatggact gcaacatagc ctggcgttgc
     1741 tcaaacagcg tggagatttc gtagggttcc aggccgaaaa ggattttttt cagtacctta
     1801 accagaatgc aggtgccaat cagcccgacc agcatgccaa ataataccga ccagatgatg
     1861 ctccagcgac tgtcattgat ctgttgggtc acgcgactta actcaaggcc gatcgccacc
     1921 acgccaattt gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa agcctgcgcc
     1981 agaaaaccgc gattgatagc gacattttct tcgccattca gcgctttaag gatgtcatca
     2041 cctttaaatg gctgaccaat acgctgggct tcaggatgcg agtagcgaag actttgcata
     2101 tcggtaacga caataaacag cagatcgttg cgtttgcgca cggcttccgc gatggcctgg
     2161 attccactct cctgtggttt tttctgcaag ccctgacgga tttccggcga gtcggcgagg
     2221 gtacgcgcta ctgccagtgc cttgttagct agcccgtctc gcgtcatatc actgatttgc
     2281 gagaagtaaa tcagatgcac caccaatagc accgagaaca gtaccgcgct gaccattaag
     2341 atcactgtgg tactcaattt catcggacgt ttgcgtaaca tgcggtaggg caatgaatgt
     2401 ctcatcagct tccttgtgtg acaaatttct taagcattat ctctgatgag gcgggtaatt
     2461 caaagggagt aagaatgatt ggcatatagg ggaagagact ctggcaacgg aaactgccag
     2521 tgctgtatga agattccagg gctatgctta tagtgaaaac catactgatg agagagggaa
     2581 ggtcatggat caggcgctac tggacagggg ttatcgctgt tataccggcg aaaagatcga
     2641 tgtctatttc aacactgcga tatgtcagca ttctggcaat tgcgtacgtg gcaacggcaa
     2701 gttatttaat ctcaaacgaa agccgtggat catgccggat gaagtcgacg tcgccactgt
     2761 ggttaaagtg attgatacgt gcccgagcgg cgcgctgaaa taccgtcata aataagcgag
     2821 ggtaaaatgg aaatacgcga aggccacaat aaattttaca ttaatgacga acagggcaag
     2881 caaatcgctg aaattgtctt tgtgccgacc ggagagaatt tagcgattat cgaacatacc
     2941 gatgtcgatg aaagcctgaa agggcaaggg attggtaaac agctggttgc gaaagtcgtg
     3001 gaaaaaatgc gtcgggaaaa acgaaaaatt atcccactat gcccatttgc gaaacacgaa
     3061 tttgataaaa cgcgggagta tgatgatatt cgcagttgat gggagagtac agagtcacga
     3121 tatttttcat gctctccgcg gtgtgatgca ggagagcatc tgaagggtag ggggatgcac
     3181 aaagaatggg cagagagcgc gtttttttgt cccaagtcat cccctttact gagcaaaaaa
     3241 aagaatatct cctatatgag aatcatcaat cggggttaat aagttttgcg tcccc
//
LOCUS       DAZUCC010000341         3291 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZUCC010000341 DAZUCC010000000
VERSION     DAZUCC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.343"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..264
                     /locus_tag="ACHY09_004938"
     CDS             <1..264
                     /locus_tag="ACHY09_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFS3887769.1"
                     /translation="AVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKAR
                     KLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            265..1323
                     /gene="rsxD"
                     /locus_tag="ACHY09_004939"
     CDS             265..1323
                     /gene="rsxD"
                     /locus_tag="ACHY09_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFS3887770.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGV
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1327..1947
                     /gene="rsxG"
                     /locus_tag="ACHY09_004940"
     CDS             1327..1947
                     /gene="rsxG"
                     /locus_tag="ACHY09_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFS3887771.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1951..2646
                     /gene="rsxE"
                     /locus_tag="ACHY09_004941"
     CDS             1951..2646
                     /gene="rsxE"
                     /locus_tag="ACHY09_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFS3887772.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            2646..3281
                     /gene="nth"
                     /locus_tag="ACHY09_004942"
     CDS             2646..3281
                     /gene="nth"
                     /locus_tag="ACHY09_004942"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS3887773.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT          778 a          834 c          886 g          793 t
ORIGIN      
        1 gccgtcgaag cggctattgc ccgtgccaaa gcgcgcaagc tggaacagca acaggctaac
       61 gcagaaccag aagaacaggt tgatccgcgc aaagccgccg tcgaagctgc tattgcccgt
      121 gccaaagcac gcaagctgga acagcaacag gctaatgcgg agccagaaga acagattgat
      181 ccgcgcaaag cggcagttgc tgcggctatt gcccgcgttc aggccaaaaa agccgcccag
      241 caaaaggttg taaacgagga ctaaatggta ttcagaatag ctagctcccc ttatacccat
      301 aaccagcgcc aaacatcgcg cattatgctg ttggtgttgc tcgcagccgt gccaggaatc
      361 gcagcgcaac tgtggttttt tggttggggt actctcgttc agatcctgtt ggcgtcggtc
      421 agtgctctgt tagccgaagc tctcgtactc aaactacgca agcagtcggt agccgcaacg
      481 ttgaaagata actcagcatt gctgacaggc ttattgctgg cggtaagtat tccccccctc
      541 gcgccatggt ggatggtcgt gctgggtacg gtgtttgcgg tgatcattgc taaacagttg
      601 tatggcggtc tggggcaaaa cccgtttaat ccggcaatga ttggttatgt ggtcttactg
      661 atctccttcc cggtgcagat gaccagctgg ttaccgccac atgaaattgc ggtcaacatc
      721 cctggtttta tcgacgccat acaggttatt ttcagcggac ataccaccag tggtggtgat
      781 atgaacacgc tacgcttagg tattgatggc attagtcagg cgacaccgtt ggatacattt
      841 aaaacctctg tccgtgccgg tcattcggtt gaacagatta tgcaatatcc gatctacagc
      901 ggtatgctgg cgggcgttgg ttggcaatgg gtaaatctcg cctggctggc tggcggcgtg
      961 tggttgctat ggcagaaagc gattcgctgg catattcccc tcagcttctt agtaacgctg
     1021 gcgttatgcg caacgttggg ctggttgttc tcaccagaaa cactggcatc accgcaaatt
     1081 catctgctgt ctggtgcgac tatgctcggc gcattcttta ttttgactga cccggttact
     1141 gcttctacga ccaatcgtgg tcgtctgatg ttcggcgcgc tggcgggttt attagtctgg
     1201 ttgatccgca gtttcggcgg ctatcctgac ggcgtggctt ttgccgtcct gctggcgaac
     1261 atcacggttc ctctgatcga ttactacacg cgtccgcgcg tctacggcca tcgcaaaggg
     1321 taaaccatgc tgaaaactat ccgaaaacac ggcattacac tggcactatt tgcagcgggt
     1381 tctacagggt taactgcggc catcaaccag atgaccaaaa cgacaattgc tgaacaggcc
     1441 agtctgcaac aaaaggcgtt atttgatcag gtgctgccag ccgaacgcta taacaatgcg
     1501 ctggcacaga gttgctatct ggtcactgcg ccagagttag gtaaaggtga gcatcgggtt
     1561 tacatcgcca aacaggatga caaaccggta gccgccgttc tggaaacaac cgcgccagat
     1621 ggctattccg gtgcgattca gctgctggtg ggagccgatt ttaacggcac ggtacttggc
     1681 acgcgcgtga cagagcacca cgaaacgcca gggcttggcg ataaaatcga actgcgcctt
     1741 tctgactgga tcacccattt tgcgggtaaa aaaatcagtg gtgcggatga tgcaaactgg
     1801 gcggtgaaga aagatggtgg tgatttcgac cagttcaccg gcgcgacgat tactccccgc
     1861 gcggtggtaa atgcggtaaa acgcgccgga ttgtacgctc agacgttacc ggcacaactt
     1921 tctcaacttc ctgcctgtgg agaataaagc atgagcgaaa ttaaagacgt tattgttcag
     1981 gggttgtgga aaaacaactc tgcgctggtc cagttgctcg gcctctgtcc gctattggcg
     2041 gtcacgtcca ctgccactaa cgctctgggt ttaggacttg cgactacgct ggtactgacg
     2101 ctgaccaact tgaccatttc gacgctgcgt cactggacgc cagccgagat ccgcattccc
     2161 atttacgtga tgatcatcgc ctcggtggtc agcgctgtac agatgctgat caacgcctac
     2221 gcctttggcc tgtatcaatc attagggatt tttattccgc tgattgtcac taactgtatc
     2281 gttgtgggcc gcgctgaagc cttcgccgcc aaaaaaggtc cggcgctttc ggcactggac
     2341 ggcttttcaa ttggtatggg cgcaacctgc gccatgttcg tgctgggttc actacgcgaa
     2401 attatcggca atggcacatt gtttgacggt gcagatgcgc tgttaggtag ctgggcaaaa
     2461 gtattacgcg tggagatatt ccacaccgac tcccctttcc tgctggcgat gctgccacca
     2521 ggtgcattta ttggcctggg actgatgctg gcagggaaat acctgattga tgaaagaatg
     2581 aaaaagcgcc gtgctgaagc agctgccgaa cgtgcattgc caaacggtga aacagggaat
     2641 gtctgatgaa caaagcaaaa cgcctggaga tcctcactcg cctgcgtgag aacaatcctc
     2701 atcccaccac cgagcttaat ttcagttcgc cttttgaatt gctgattgcc gtactgcttt
     2761 ccgctcaggc gaccgatgtc agtgttaata aggcgacggc gaaactctac ccggtggcta
     2821 atacacctgc agcgatgctt gaactgggcg tcgaaggggt gaaaacctat atcaaaacca
     2881 ttgggcttta taacagcaaa gcagaaaata tcatcaaaac ctgccgtatc ttactggaac
     2941 agcataatgg cgaggttccg gaagatcgtg ctgcgcttga agccctgcct ggtgtaggtc
     3001 gtaaaacagc caacgtcgta ttaaacactg cattcggctg gcctactatt gctgtcgaca
     3061 cgcacatttt ccgcgtttgt aatcgtactc aatttgcgcc ggggaaaaac gtcgaacagg
     3121 tagaagaaaa gctactgaaa gtggttccgg cagagtttaa agtcgactgc caccattggt
     3181 tgatcctgca cgggcgttat acctgtattg cccgcaagcc tcgctgtggc tcttgtatta
     3241 ttgaagatct ttgtgaatac aaagagaaag ttgacatctg aagaaaaggg g
//
LOCUS       DAZUCC010000342         3242 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZUCC010000342 DAZUCC010000000
VERSION     DAZUCC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.344"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>3242
                     /locus_tag="ACHY09_004943"
     CDS             <1..>3242
                     /locus_tag="ACHY09_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFS3887774.1"
                     /translation="PTELNDIRLVRETIRTLGQRVREGYTPREDGAGASSQYSRSGKP
                     DPFKADAYDAKQFASEVRRIAGLQSVPRGGEVRVGDTPPVLRALGAPNKELVMPAHVV
                     HKATRPELREHYVSVETMADLPQLLADPVAVMTSKTEHDSLVTLIEAKDRRGAPVIVA
                     IHMRAKGAVAEVNKIASVYGKDNRIALQEQLDTSLLYLNKEKAADWLLSAGLQLPGAN
                     TINGSSGFKVLHPEDIRKGAYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQENIRK
                     TGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLANYKISQADLDEYLYAR
                     HAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVDDMLARR
                     RELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQAMG
                     RASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDTMRT
                     IAERKDQETGETRREVVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLMRAMKNMGP
                     ETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKGL
                     DNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGWFNMGDLE
                     GQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHARDAGLSR
                     QQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGEGPLLERL
                     RWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNLVIMKSV
                     FGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAFSPIGSE
                     TSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRSTPE
                     AYKAFASWLNAFSGGSQYRPGAVDVTPESLKYWIDYISGGTGRFISKTTDAAVKSLNG
                     IDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTA"
BASE COUNT          808 a          783 c          971 g          680 t
ORIGIN      
        1 ccgacggaac tgaacgatat cagacttgtt cgcgagacca tccgtacgtt aggccagcgt
       61 gtgcgggaag gttacacgcc gcgtgaggat ggcgcgggcg catcgtctca gtactcccgt
      121 agtggtaagc cggatccgtt taaggctgac gcctatgatg caaagcagtt tgcttcagaa
      181 gtgcgtcgca ttgccggatt acaatcggtt ccacgaggtg gagaggtgcg ggttggtgac
      241 actccgccag tgttaagagc acttggcgca ccaaataaag agctggttat gccagctcat
      301 gtggttcaca aggccaccag accagaatta cgtgagcact atgtttctgt tgaaacgatg
      361 gctgacttac cgcaattgct tgccgacccg gttgcggtga tgacatcaaa gacggaacat
      421 gactcactgg taactttgat tgaggctaag gacagaagag gtgcacctgt cattgttgca
      481 attcacatgc gtgctaaagg ggcggtagct gaggttaata agattgccag tgtctacgga
      541 aaggacaaca ggatcgcttt gcaggaacag ttggatactt ccttgctgta tctgaataaa
      601 gaaaaagccg ctgactggct tctttcagcc gggctccaat tgcccggggc gaacaccatc
      661 aacggctctt ctggatttaa agtactacat cctgaagata ttcgcaaggg ggcgtattac
      721 tcccgcacca gcagtctgac accggaagag accattgcat cgcgttttgt tcgccagatg
      781 caggataaat tccaggtgct gaaggcggtg caggagaata tccgtaaaac tggcggaaaa
      841 atagatgaca gcaataacgc ctatatggct gaagaactct tccacgggaa ggcggaaaac
      901 gacctgaacg taatgaagga gcgctacgtt cagccgctgg ccaaattgct ggcgaactac
      961 aaaatctctc aggccgatct ggatgagtac ctctacgccc gtcacgcgcc ggaacgtaac
     1021 gcacatattg cgaaaatcaa ccctaaaatg ccggacggcg gttcggggat gaccaacgcg
     1081 gaggcggcgg aaatcatgca gcgtgtgcgt aacagcggca aacaggcaca gtatgaccgt
     1141 ctggcaggga ttgttgacga tatgctggcc cgtcgccgtg agcttatccg tgaggccgga
     1201 cttgaagaga acggtgtggt ggatgcctgg cagaacgcct accgttacta cgttcccctg
     1261 aaaggtcagg atgttgacgg tgtggtgtca cttccccgta caggtaaggg cttcaccatc
     1321 ggcggacgtg aaagcaggca ggccatgggg cgtgcatccc gtgcacagtc tccgtccact
     1381 caggcgatac aggacctgag cgaatcgctg atccgccatc gcaaaaacga agtgggtaac
     1441 gccttcctga aactggtgca ggataatccc gacaaggatt actggcaggt attcaccgat
     1501 gacagaccgg ataccatgcg gacgattgca gagcgcaagg accaggaaac tggtgaaacc
     1561 cgtcgcgaag ttgtcgaacg ccctgtaccg atggcaatga tggcagaccg ctacttcacc
     1621 accaaaaaga acggcaaaac gtactacatc aaactccatg acccgcgcct gatgcgtgcg
     1681 atgaagaata tgggaccgga aaccagtaac gccgtaatcc gtacgctggg gaaagttaac
     1741 cgcttcctgg caacggtgaa cacgtcgtat aacccggaat tcctggtcag taacttcatc
     1801 cgtgacgtgc agacggcggt gatgaacctg aaggctgagc agggaaggag cgacggtaaa
     1861 ctgaaagggc tggataactt atccgccctg gctgtagtca aagacagccg ttctgccatg
     1921 tcagccgtat acgccagtct gcgtggtaaa accctcacgg gcaaaggtgc acagtggcag
     1981 aaggtgtgga aagagtttgt tgaggacgga ggtaaaaccg gttggtttaa catgggtgac
     2041 cttgaaggcc agcagaagga aatggatcgc cttgtttcgc tggcgaaggg aggatggaaa
     2101 ggccagagta tcggtgcatg gaattcgttc cttaaccttg tcgaggatgc caacggcgcg
     2161 gttgaaaacg cactgcgtct gtcagcttat aaacacgccc gtgatgccgg tttgtcacgc
     2221 cagcaggcgg cgtctcttgc caaaaacatg acggtgaact ttaaccgtcg tggtgagcag
     2281 ggggcgctga tgaactcgct gtacatgttc gccaacgcca gtattcaggg aacggcaaat
     2341 ctggtgagaa cgctcggaca tcttaatggt gagggacctt tactggagcg ccttcgctgg
     2401 aagaatctga atgttccgca gaaaatcgcg cttgccgctg tgggagcagg ttatctgctt
     2461 ggctcgctta accgcagtgt ggcgggtgag gatgatgacg gggttaactg gtatgacaag
     2521 gtgccgtctc atgtgaaaga gcgtaacctc gtcattatga aatcggtgtt cgggggcaag
     2581 gccggagagt actggagtat tcctctgcct tacgggtaca acgttttctt cctgctcggg
     2641 cataccgctg aaggtgtggc ggcgggtgac ctgacggcgt cccgtgctgc cggtaatgtt
     2701 gttggtggtg tgcttggtgc attcagcccg attggcagtg agacgtcgga aacactgtcc
     2761 ggggcattgc tgaaaaatgc agcgccgacc attctgcgtc cgtttgcgaa ccttgccatg
     2821 aatgaaaact tcatgggggc gcagatttac caggagaaca tgccgtttgg tacaccaaaa
     2881 cctgacagcc agctgggaag acgttcaacg ccagaagcgt acaaggcgtt tgcatcctgg
     2941 ctgaatgcgt tctcaggtgg cagccagtac cgtcccggcg cggtggatgt cacgccggaa
     3001 tcactgaaat actggattga ctatatctct ggtggtacag ggcgcttcat ttccaaaacc
     3061 acggatgcgg cggtgaaatc gctgaatggt attgatatac cggagcagca ggtgcctttc
     3121 ctggggaaaa tttcaggtga ggtgatgccg tatgctgacc agcagaagat gtacgaccgg
     3181 atgacagagg ttgcgcagta ccacgcagag ctgaagagtc tgaccggtgc agaaagaacg
     3241 gc
//
LOCUS       DAZUCC010000343         3208 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZUCC010000343 DAZUCC010000000
VERSION     DAZUCC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.345"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            175..693
                     /locus_tag="ACHY09_004944"
     CDS             175..693
                     /locus_tag="ACHY09_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFS3887775.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            903..3011
                     /locus_tag="ACHY09_004945"
     CDS             903..3011
                     /locus_tag="ACHY09_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFS3887776.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQVIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLMVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          810 a          808 c          919 g          671 t
ORIGIN      
        1 agatttaatg attttaaaaa tagtttttac gacaaccgta aattctagct cggtttgtat
       61 gtaaaaaata ctgaaaaatg atgcggttcc gtgaagagag ctgcatctgc ctgtctggga
      121 ttaacggcag gtcattgtaa gtagtaatta accacgttaa caaggagttt acaaatgcca
      181 acaccgtgtt atatctccat cactggtcag acccagggaa atattactgc aggtgcattt
      241 accgctgatt ctgtcggcaa catctatgtg caggggcacg aagacgagat gctggtgcag
      301 gaattcctgc ataacgttac tgtcccgacc gatcctcagt ccggccagcc tgcgggtcag
      361 cgtgctcaca agccgttcat ttttaccgtg gcgctgaaca aagctgttcc gctgatgtac
      421 aacgcactgg cctccggcga aatgctgcca accactgaac ttcactggtg gcgtacctct
      481 gtggaaggca aacaggagca ttatttcacc acccgcctga ccgattcgac gattgtcgat
      541 atgaaactgc atatgccgca ttgccaggat ccagcgaaac gcgagttcac ccagttgctc
      601 gaagtttcac tggcctaccg caaaatagag tgggaacacg tcaagtctgg cacctccggt
      661 gccgacgact ggcgcgcacc gctggaagca taagttaagc caacagcatc cggctatcag
      721 gccggatgct gtttttgtat tacgccgtaa aaagaaaggt tgtcagtaag ggattacgtt
      781 cacgggagat ggcggatgcc ggaaacttcg gcggtagcgt gcctgaacgc aagcgtaaca
      841 ctgtactgtt acagccgctt taacagcgaa ctgtctgttt ttccttaaac ccggaggttg
      901 ctatgtcaac cggattacgt ttcacgctgg aagtggacgg tctgccaccg gacgcgtttg
      961 cggtggtctc ctttcatctg aaccagtcac tctcttcgct cttttccctc gatctctctc
     1021 tggtcagcca gcagtttctc tcccttgaat ttgcgcaggt gctggacaaa atggcctacc
     1081 tgacggtatg gcagggcgat gacgtacagc gccgggtgaa aggtgtggtg acctggtttg
     1141 aactggggga gaacgacaaa aaccagatgc tgtacagcat gaaggtgtgc ccaccgctgt
     1201 ggcgcacagg gctgcgccag aacttccgta tcttccagaa tgaggacatc gaaagcatac
     1261 tcgcgacgat cctgaaagaa aacggggtga ccgagtggag tccgctgttc agcgagccgc
     1321 atccttcccg tgagttttgt gtccagtacg gcgaaaccga ttacgatttc ctgtgccgga
     1381 tggcggcgga ggaaggcatc ttcttttatg aggagcacgc gcaaaaaagt accgaccaga
     1441 gcctggtcct gtgcgacacc gtgcgttatc tgccggagtc ctttgagatc ccctggaatc
     1501 cgaacacccg taccgaggtg agcaccctat gcatcagcca gttccgctac agcgcacaaa
     1561 tccgcccatc ttccgtggtg accaaagact acacctttaa acgacccggc tgggcagggc
     1621 gttttgatca ggaaggccag caccaggatt accagcgcac acagtatgaa gtgtatgact
     1681 accccggacg tttcaagggt gcccacgggc agaactttgc ccgctggcag atggatggct
     1741 ggcgaaacaa cgcagaagtg gcgcgcggaa caagccgttc gccggagata tggccgggac
     1801 ggcgaattgc gctgacgggg catccgcagg cgaacctgaa ccgggaatgg caggtggtgg
     1861 caagtgaact gcacggcgag cagccgcagg cggtgccggg acgcaggggt tcaggtacca
     1921 cgctgaataa ccactttgcg gtaataccgg cagacagaac atggcggcca cagccgttgc
     1981 tgaaaccgct ggtggacggc ccgcagagcg ccgtcgtgac gggaccggca ggagaagaaa
     2041 tcttctgtga tgaacatggc cgcgtgcggg tgaaatttaa ctgggaccgc tataacccgt
     2101 caaaccagga cagttcatgc tggatccgtg tggcacaggc gtgggcaggc accggctttg
     2161 gcaacctggc gataccgcgt gtgggtcagg aagtcattgt ggacttcctc aacggcgatc
     2221 cggaccagcc gattattatg gggcgtacct accaccagga aaaccgcacc cccggcagcc
     2281 tgccgggaac aaagacgcag atgaccattc gttcgaaaac ctataagggc agcgggttta
     2341 atgaactgaa gtttgacgat gcgacaggga aagaacaggt ctacatccac gcgcaaaaga
     2401 acatgaacac cgaggtgctg aataaccgga ccacggatgt gataaacaac catgctgaga
     2461 ccatcggcaa caatcaggtg attgcggtta ccaacaatca gatacagacg gtgggcgtta
     2521 accagataga gacggtgggc agtaaccaga tcattaaagt gggctctgtt caggtggaaa
     2581 cgattggact tgttcgtgcg ctgaccgtgg gcgtggcgta tcagacgacg gtaggtggca
     2641 ttatgaacac ctcggtggcc ctgatgcagt cctcgcagat cggtttgcat aaatcgttga
     2701 tggtcgggct gggttatgac gtcaaagtcg gaaataacgt taccttcacc gttggtaaaa
     2761 cgaaaaagga tgataccgga cagaccgcga tttactccgc cggtgagcat ctggaactct
     2821 gctgtggtaa ggcaaggctg gtgctgacta aggacggaca aatttttctc aacggcacaa
     2881 aaattcattt gcagggtaag gagcaggtta atggtgactc actgttgatt aactggaact
     2941 gtgcagcctc gaaatcccca ccgaagaccc ctgatgaaaa gcaggatacg ccggatatga
     3001 gagagtactg atcccgtacc tgttcggtat tcagtaacaa acaagcatta aaaataaaca
     3061 gaagaaggaa ttgaatcatg agcggaaaac cagcggcgcg tcagggagat atgactcagt
     3121 atggcggtcc cattgtccag ggttcggcag gtgtaagaat tggcgcgccc accggcgtgg
     3181 cgtgctcggt gtgtccgggc gggatgac
//
LOCUS       DAZUCC010000344         3208 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZUCC010000344 DAZUCC010000000
VERSION     DAZUCC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.346"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..1224
                     /gene="proB"
                     /locus_tag="ACHY09_004946"
     CDS             121..1224
                     /gene="proB"
                     /locus_tag="ACHY09_004946"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS3887777.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1236..2489
                     /gene="proA"
                     /locus_tag="ACHY09_004947"
     CDS             1236..2489
                     /gene="proA"
                     /locus_tag="ACHY09_004947"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS3887778.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAGALAQLQTGPAKVVAVKTEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     SRGPMGLEALTTYKWIGIGDYTIRA"
     gene            2604..2679
                     /locus_tag="ACHY09_004948"
     tRNA            2604..2679
                     /locus_tag="ACHY09_004948"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2637..2639,aa:Thr,seq:cgt)
     gene            complement(2694..2851)
                     /locus_tag="ACHY09_004949"
                     /pseudo
     CDS             complement(2694..2851)
                     /locus_tag="ACHY09_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835868.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            2900..3085
                     /locus_tag="ACHY09_004950"
     CDS             2900..3085
                     /locus_tag="ACHY09_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887779.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
BASE COUNT          775 a          782 c          905 g          746 t
ORIGIN      
        1 cttgctgatc ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga
       61 cagtcctgct aaaacgttcg tttgatatca ttttttccta aaattgaatg gcagagaatc
      121 atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac aggcggatcg
      181 cgccgcctga accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca gttacatgcc
      241 gccgggcatc ggattgttat tgtgacgtcg ggtgcgattg ccgccggacg tgagcacctg
      301 gggtacccgg aattgccagc gactatcgct tcaaaacagc tgctggcggc ggtagggcag
      361 agtcgactga ttcaactgtg ggaacagctg ttttcaattt acggcattca tgtcgggcag
      421 atgttgctga cccgtgctga tatggaagat cgtgaacgct tcctgaacgc ccgcgatacc
      481 ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatta atgagaacga tgctgtcgct
      541 acggcagaga ttaaggtcgg tgataacgat aacctttctg cgctggcggc gattctggcg
      601 ggtgccgata aactgttgct gctgaccgat caaaaaggtt tgtataccgc tgacccgcgc
      661 agcaatccgc aggcagaact gattaaagat gtgtacggca ttgatgacgc actgcgcgcg
      721 attgccggtg atagcgtttc aggcctcgga actggcggta tgagcaccaa attgcaggcc
      781 gcggacgtgg cttgccgtgc gggtatcgac accattattg ccgcgggcag caagccgggc
      841 gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc ccaggcgact
      901 ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc cggcgggtga aatcacggta
      961 gatgaagggg caaccgccgc cattcttgaa cgcggcagct ccctgttgcc gaaaggcatt
     1021 aagagcgtaa ctggcaattt ctcgcgtggt gaagtcatcc gcatttgcaa cctcgaaggt
     1081 cgcgatatcg cccacggcgt cagtcgttac aacagcgatg cattacgccg cattgccgga
     1141 caccactcgc aagaaattga tgcaatactg ggatatgaat atggcccggt tgccgttcac
     1201 cgtgatgaca tgattacccg ttaaggagct ggccgatgct ggaacaaatg ggcattgccg
     1261 caaagcaagc ctcgtataaa ttagcgcaac tctccagccg cgaaaaaaat cgtgtgctgg
     1321 aaaaaatcgc cgatgaactg gaagcacaaa gcgaaatcat cctcaacgct aacgcccagg
     1381 atgttgctga cgcgcgtgcc aatggcctta gcgaagcgat gcttgaccgt ctggcactga
     1441 cgcccgcacg gctgaaaggc atcgccgacg atgtgcgtca ggtgtgtaat ctcgccgatc
     1501 cggtggggca ggtaatcgat ggcggcgtac tggacagtgg tctgcgtctg gagcgccgtc
     1561 gcgtgccgct gggggtgatt ggcgtgattt atgaagcgcg cccgaacgtg acggtagatg
     1621 tcgcttcgct gtgcctgaaa acaggtaacg cggtgatcct gcgcggcggc aaagaaacgt
     1681 gtcgcactaa cgctgcaacg gtggcggtga ttcaggacgc cctgaaatcc tgcagcttac
     1741 cggcgggtgc cgtgcaggcg attgataatc ctgaccgcgc gctggtcagt gaaatgctgc
     1801 gtatggataa atacatcgac atgctgatcc cgcgcggtgg cgctggcctg cataaactgt
     1861 gccgcgaaca gtcgacgatc ccggtgatca caggtggtat aggcgtatgc catatttacg
     1921 ttgatgaaag tgcagagatc gctgaagccc tgaaagtgat cgtcaacgcg aaaactcagc
     1981 gaccgagcac atgtaatacg gttgaaacgt tgctggtgaa taaaaacatc gccgatagtt
     2041 tcctgcccgc attaagcaaa caaatggcgg aaagtggcgt gacgttacac gctgatgcag
     2101 gtgcactggc gcagttgcag acaggccccg cgaaggtggt tgctgttaaa accgaagagt
     2161 atgacgatga gtttctgtca ttagatttga acgtcaaaat cgtcagcgat cttgacgatg
     2221 ccatcgccca tattcgtgaa cacggcacgc aacactccga tgcgatcctg acccgcgata
     2281 tgcgcaacgc ccagcgtttt gttaacgaag tagattcctc cgctgtttac gttaacgcct
     2341 ctacgcgttt taccgacggc ggccagtttg gactgggtgc ggaagtggcg gtaagcacac
     2401 aaaaactcca ctcgcgtggc ccaatggggc tggaagcact gaccacttac aagtggatcg
     2461 gcattggtga ttacaccatt cgtgcgtaaa taaaaccggg tgatgcaaaa gtagccattt
     2521 gattcacaag gccattgacg catcgcccgg ttagttttaa ccttgtccac cgtgattcac
     2581 gttcgtgaac atgtcctttc agggccgata tagctcagtt ggtagagcag cgcattcgta
     2641 atgcgaaggt cggaggttcg actcctctta tcggcaccat ttaaatcaat aagttacaca
     2701 tcactaatac cttccttatt ttttattggg acagatttgg gaccgatggg ttcaggatcg
     2761 aatctattta ccgtgcgtgt tcggtaaggt gattaggtgc gaggtgagca tatcgacgaa
     2821 ccatttcgag agtttcccat ccgctcattt ttaagtgcaa gcagggaaac accggaccaa
     2881 accaatcagc tgttcgaaaa tggcaagcgg attaatatgc catggcggct ttgagccgca
     2941 cgcatggggt gtggcaagcc tgatggatgt tactgtgctt gtggtggcgt ctttgctggt
     3001 ctggttgttg gttaatcgcg aaaatcatta tgccagagga ataccacagg acgttcaggt
     3061 tttgcataaa aaagcccacc attaaggtgg gcaaaaggaa tgatttatat tctctgagag
     3121 aaacattaca tttctccttg tcacttctaa catgaaaata tggagaaata atggagctta
     3181 tattaattgg cgattaggta accatgtg
//
LOCUS       DAZUCC010000345         3180 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZUCC010000345 DAZUCC010000000
VERSION     DAZUCC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.347"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..85
                     /locus_tag="ACHY09_004951"
     CDS             <1..85
                     /locus_tag="ACHY09_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HFS3887780.1"
                     /translation="INVKSNNANNWHLTIIDDHGNYISDKI"
     gene            101..2581
                     /gene="yehB"
                     /locus_tag="ACHY09_004952"
     CDS             101..2581
                     /gene="yehB"
                     /locus_tag="ACHY09_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFS3887781.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSIQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPFRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCAITFGKEIDESRNYICQ"
     gene            2597..>3180
                     /locus_tag="ACHY09_004953"
     CDS             2597..>3180
                     /locus_tag="ACHY09_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFS3887782.1"
                     /translation="MEIRIMLFIVMMMVMPVSYATCYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLALSYNPITTT"
BASE COUNT          889 a          601 c          805 g          885 t
ORIGIN      
        1 tattaatgtc aaaagtaata atgcgaataa ctggcatctg accattatcg atgaccatgg
       61 caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa tgaccccact
      121 tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg aagaaacctt
      181 tgatacccat ttcatgatag gtggaatgaa agaccagcag gttgcaaata ttcgtcttga
      241 tgataatcaa cccttgccgg ggcagtatga catcgatatt tatgtcaata agcaatggcg
      301 cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat caagggaaat
      361 tatcaagcgg ttaggcatta ataccgataa ctttgccagc ggtaagcaat gtttaacatt
      421 tgagcaactt gttcagggtg ggagctatac ctgggatatc ggagtttttc gtctcgattt
      481 cagtgtcccg caggcgtggg tggaagaact ggaaagtggc tatgttccac cggaaaactg
      541 ggagcggggt attaatgcgt tttatacctc ttattatgtg agtcagtatt acagcgacta
      601 taaagcgtcg ggtaatagca agagtacata tgtacgtttt aacagcgggt taaacttact
      661 ggggtggcaa ctgcattctg atgccagttt cagtaaaaca aatagcaatc caggggtgtg
      721 gaaaagcaat accctgtatc tggaacgtgg atttgcccaa cttctcggca cgcttcgcgt
      781 gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg gtgtgcggtt
      841 gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttactccac gggtgcaggg
      901 gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg tttatcagaa
      961 agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg gtggagcaga
     1021 tcttgatgtc agcgtgaaag aggcggacgg ttcggtaacc acctatctgg tgccttatgc
     1081 agcagtgccg aatatgctgc aacccggcgt gtcgaaatat gattttgcgg caggtcgtag
     1141 ccatattgaa ggggcgagca aacaaagtga ttttgttcag gcgggttatc agtatggttt
     1201 taataactta ttgacgctgt atggcggatc gatggtcgcg aataattatt acgcgttcac
     1261 tttggggact ggctggaata cacgcattgg tgccatttcc gtcgatgcca cgaagtcgca
     1321 tagtatacaa gacaacggcg atgtgtttga cgggcaaagt tatcaaattg cctacaacaa
     1381 atttgtgagc caaacgtcga cacgttttgg tctggcggcc tggcgttatt cgtcgcgtga
     1441 ttatcggaca tttaatgatc acgtatgggc aaacaataaa gataattatc gccgtgatga
     1501 aaacgatatc tatgacattg ccgattatta ccagaacgat tttggccgta aaaatagctt
     1561 ttctgccaat atgagccagt cattgccaga aggctggggt tctgtgtcct taagtacgtt
     1621 atggcgagat tactgggggc gtagcggcag cagcaaggat tatcagttga gttattccaa
     1681 caacctgcga aggataagct ataccctcgc ggcaagccat gcttatgacg agaatcatca
     1741 tgaagagaaa cgttttaata tttttatatc gattcccttt gattggggtg atgacgttac
     1801 gacgcctcgt cggcaaatat atatgtctaa ctcaacgacg tttgatgatc agggggttgc
     1861 ctcaaataat acgggattat caggaaccgt tggaagccgg gatcagttta actatggggt
     1921 caacctgagt tatcagcatc agggaaatga aacgacagct ggagcgaatt taacctggaa
     1981 cgcgccggtg gcaacggtga atggcagtta tagtcagtcg agtacttatc gacaggctgg
     2041 agccagtgtt tcagggggca ttgtcgcctg gtcgggtggc gttaatctgg cgaaccgtct
     2101 ttccgaaacg tttgctgtaa tgaatgcgcc aggaattaaa gatgcttatg tcaatgggca
     2161 aaaatatcgc acaacaaacc gtaatggagt ggtggtatac gacggaatga caccttatcg
     2221 ggaaaatcac ctgatgctgg atgtgtcgca aagcgatagc gaagcagaat tacgtggcaa
     2281 ccggaaaatt gccgcccctt ttcgcggcgc ggttgtactg gttaattttg ataccgatca
     2341 gcgcaagccc tggtttataa aagcgttaag aacggatggg caaccattaa cgtttggtta
     2401 tgaagtcaat gatatccatg gtcataatat tggtgttgtc ggccagggaa gccagttatt
     2461 tattcgcacc aatgaagtac cgccatcggt taatgtggca attgataagc aacaagggct
     2521 ttcatgcgca atcaccttcg gtaaagagat tgatgaaagt agaaattata tttgccagta
     2581 atagtcaggt ggttctatgg aaattcgcat aatgctattt atagtaatga tgatggttat
     2641 gcctgtgagc tatgcgacat gttatagtga gttatctgtt cagcacaatt tggttgttca
     2701 gggggatttt gcacttactc aaacacaaat ggcgacatat gagcataatt ttaatgattc
     2761 gtcatgcgtg agtacaaata ctatcacacc tatgagcccg tcggatatta ttgttggact
     2821 ttataacgat accattaaat taaatttaca ttttgaatgg accaataaaa acaacattac
     2881 gttgtcaaat aatcagacca gtttcaccag tggttattcg gtaacggtga cacctgcggc
     2941 cagtaatgca aaagtgaatg tttctgcggg gggcggcggt tcagtgatga ttaatggtgt
     3001 tgcaacatta tccagtgctt catcatcgac acgcgggagt gccgcagtac aatttctact
     3061 gtgtttatta ggtggcaagt catgggatgc atgtgtaaat agctacagaa atgcattggc
     3121 acaaaatgca ggtgtctatt cctttaattt ggcattgtca tacaacccga taaccacaac
//
LOCUS       DAZUCC010000346         3174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZUCC010000346 DAZUCC010000000
VERSION     DAZUCC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.348"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..222
                     /locus_tag="ACHY09_004954"
     CDS             <1..222
                     /locus_tag="ACHY09_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887783.1"
                     /translation="DDEIHFLSFQDSNRHELTNEPEEDADDLSVGYLGSSWRTELSWT
                     TSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            complement(537..959)
                     /gene="subB"
                     /locus_tag="ACHY09_004955"
     CDS             complement(537..959)
                     /gene="subB"
                     /locus_tag="ACHY09_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFS3887784.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(976..2019)
                     /gene="subA"
                     /locus_tag="ACHY09_004956"
     CDS             complement(976..2019)
                     /gene="subA"
                     /locus_tag="ACHY09_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFS3887785.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWTELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSMNKKRDPVIRV
                     AALAQYRKGETPVLHGGGITGSRFGNGWVDIAAPGQNITFLRPDGKTGTGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEGRVLNAEKAISMFCKKN
                     YIPVRQGRMSEEL"
BASE COUNT          914 a          721 c          677 g          862 t
ORIGIN      
        1 gatgatgaaa tacattttct cagctttcag gatagtaacc gacatgaatt aactaatgag
       61 ccagaagaag atgccgatga tttatctgtc gggtatctgg ggtcatcatg gaggacagaa
      121 ttaagctgga caacgtcaca gagaagtaca cactttaatt caatggccag aaggggagtt
      181 attgctgaat gttatgcgca aaaaataaga aattatttgt agacaagttc aggatatatc
      241 tacagctctg acatgattct ctgagagaca tgacggagtg gtatggagtt gtcggtatca
      301 acaccaagat agctggtcgt taccgtttga tatcactggt tctgatacta tgtggacggt
      361 agtgtgtcac gaaggcacgc accagggacg tgagtccctg taatatcatc gacagtccca
      421 ttatggcatg tctgcaccta caacgtcatt tcgaggtcgg ctcagtgttc actcgagtct
      481 tatatcactg acgccgacac tccatccaga gtatctctgg acggaaacat cacctattag
      541 ttcttttttc tgtcagggcc aaaacattca gtcgatgtgg cgcaggtcga taacccaaca
      601 agggcattgg acgttaatgc cttcacaaag ggcggatacg tccatacccc gggttcataa
      661 taaatcctga ccaactgacc tgttgtgtaa aaatatagtg cctgattata taatgtggaa
      721 aacgaaggcc cccagacaga tgaattcttc atcgagcagg ctcttatgga agaaccacca
      781 ggttgtttcc cttcaataca aaaataaggg ttattatcta tttgccctgt atgaaactga
      841 tcaataacaa cgcctgaaaa catgccatcc cgggcatcgc cagtccactc agccatggca
      901 ggattaaggg ataaacatcc cataacacct gcacatacaa aaaacctctt aatcgtcata
      961 ttcacctccg gaattttaca gttcttcact catccttccc tgacggacag gaatataatt
     1021 tttcttgcag aacatgctaa ttgctttttc tgcattaaga accctccctt ccgtcacctt
     1081 atctgcaagt gaggggtatt tgtctgcaga ctccagcagt gtacgcttca gttctgtcgc
     1141 tgttgcacgg ggattacatg agaccatcgc tgccagaacg ccggaaacta tagctgttgc
     1201 ttccgaggtt ccactacctg tccccgtttt tccatctggt ctgaggaatg taatattctg
     1261 tcccggtgca gcaatatcaa cccagccgtt tccgaaccga cttccggtga tccctccacc
     1321 atgcaataca ggtgtttctc ctttccggta ctgtgccaga gctgccactc ttatcacagg
     1381 atcccgcttt ttattcatac tgctgaccgg atggtaagcc gcaggccata ttctctgctg
     1441 agcactcagt tttcgtatat ctgcgccatc attacccact gccgcaacaa ttaatcgctc
     1501 attattccgg cccatacggg aaagcagttc ggtccagacc gatgcggaag atatcccctt
     1561 ctggcctccc gatatattaa tgattttctc ttctcctggt gcaagaaaaa catttgacat
     1621 aatgctttca gttgctctaa tccatgagtc ctgtacaccg tcaggaataa cccttctgct
     1681 ggatatcagt gcatgaggat aaacaccata aaccccatga cgtgaggcaa tgagtgaagc
     1741 catggcagta ccatgaatat ataaagcttc agactctttt accgggaatg gcgaaccatc
     1801 ctgagtaaaa ctgaatttgg caaattcgct gtcgctgagg cctcctacaa atgctactcc
     1861 ggaatcaaca acagatacga caacaggggt attcttgtct gtgagcgaca tggttgtttc
     1921 tgtcaggcca attgcatcaa aataccaggg tttttctgcc ctgacagaca cagatataaa
     1981 aagcaggaac agaatatgcg gccataaaat cttaagcaca atatcctcca gtcagtacaa
     2041 ataaacagta ttgcaggtca atatcttaca ggaacatcac atctgaaata tcctgttttc
     2101 gacaaaagat aaacaatcac aaccaataaa gcgagtgaag cccgtctcaa aaacattaat
     2161 ttttatattt atgtatccaa tgaattaacg ctcaccttca cggtaatatg catctggatt
     2221 tctgcaacag gtcagcgata agatgctctg cctcctcctt cagagcagaa gtaaatacag
     2281 ccatgatgga taaaacttaa ccagaaaaaa taagaggagg caagtaagtt gcctggcatg
     2341 tttatgacaa taaaaagttc cgtcaggcat catggcgaac aggatatcac agcgccgttt
     2401 taccggggca atcagtgaat ggtggtgtgg ttcattggta agccccggat atcaggtcac
     2461 tgagtatatt catgaatgac cgttaaaccg gggatgcgag ctgctccccg ggaaagtcga
     2521 actgactggt caatagatat caatacgggg cagtcagtcc acttacaggt atctgacaag
     2581 gacaccttct gagcgtgcaa tgtggacggg taatacaacg atggtagcag gctggtcagc
     2641 aatcagcagt atatgcccat atttcctgag gccggatatc tgtttcctga gcttgttttc
     2701 gacgtttgct aatattttgt cgaactaatt atcagaacga ttaatgataa cagtgtggca
     2761 gggatcttta ccgatatcaa cgtaaataat gaccttaaca ggtgcgtaat tgctggattt
     2821 tgccatgctg ttaccctgtt gatacgggtt aattaaataa ctttggggaa gcaggtaccg
     2881 gcgttcacta ccgctctccc tgataatgta tcctgcatgc ctgataactt ttgccgggct
     2941 aatgtaaaat aaccctgaaa tatattaagt tgattaacag actggtgatg gaaaaaacag
     3001 gtattattta ttatataaaa atccggattt gatgcggctt gcgatatatt attttttgcc
     3061 tgtgatggga gcctgaggtt gaattatata taatgcaact ggaggttgcg ggataaagta
     3121 tatcaattat gtgacatgaa gcagaaagtt cctccccctc ttatccacag acct
//
LOCUS       DAZUCC010000347         3169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZUCC010000347 DAZUCC010000000
VERSION     DAZUCC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.349"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..1552
                     /locus_tag="ACHY09_004957"
     CDS             2..1552
                     /locus_tag="ACHY09_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS3887786.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANISGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGIRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            1593..>3169
                     /locus_tag="ACHY09_004958"
     CDS             1593..>3169
                     /locus_tag="ACHY09_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS3887787.1"
                     /translation="MKTAQRTLFAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLANVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTPKKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSDVVIDYLKKGHKIT"
BASE COUNT          981 a          664 c          683 g          841 t
ORIGIN      
        1 gatgaaaact gtatttacaa cattatcagt catattcggc gtgatatttt ctcacagtgt
       61 tctagctcag gacgttacta tttattatac gaatgacatt cacgcacatg tcaatccagc
      121 aaaaatcatc gctgttgata agaacagact tataggtggt atggctaata tttcaggcat
      181 cgttaatgaa gcgaagaaaa aaagcaaaga tgtgtttttc tttgatgcag gcgactattt
      241 taccgggccg tatatcagca ccctgaccaa aggtgaggct attattgata tcatgaacac
      301 tatgcccttt gatgcggtgt ctgttggtaa tcacgagttc gatcacggtg tgcccaatat
      361 ggtgtctcag ttatcaaaag caaaattccc cattctgctg ggaaatatct actacaccga
      421 tacgaataaa ccagtttggg atcatccgtg gaccattatc gaaaaagatg gtttaaagat
      481 tggtgtgatt ggattgcatg gcgcattcgc tttctatgat acagtcgcgg caaaagcacg
      541 tgaaggcgta gaagccagag acgaaattaa atatttaaat aaagcacttg cagaattaaa
      601 aggtaaagtc gacattaccg ttttgctaat tcacgaaggt gtcccggcgc gtcaatcaag
      661 ctttggtagt aaagatgtgg agcggctact acaggcagat attgagacag ctaaaaaagt
      721 gaatggtgtt gatgtcttaa ttaccggtca cgcacatgtc ggaacaccgc aaccaataaa
      781 agttaataac acattaattg tatctaccga tgcatacggc accaacatcg gaaaacttgt
      841 gcttgattac aatccgaaaa cgaagaaaat tgacagttat aatggtgagt taatcaccat
      901 ctttgcagat caatttaaac ctgacaccat cgttcaaaat actattgata aatggagtgc
      961 aaagcttaac aaaataacac aggaagttgt cggccattct cccgtagttt taacacgtga
     1021 gtatggtagt tcctcttcta ccggcaacct tattcttgat gcaatgatgg aaaaaacacc
     1081 tgatgccgtt gccggatttc aaaatagcgg tgggattcga gctgattttc ctaaaggcga
     1141 tatcacactg ggagatgtta ttagcacatt cccctttaat aatgacctca tcgagatgga
     1201 tttgacgggc cgcgatctca aatcattgat gacgcatgca accaatctaa ctaacggtgt
     1261 gttacaagtt tctaaaagtg ttgcggttgt ctatgacagc aaaaaacccc tcaaccaacg
     1321 gttaatctct ttcaccatta acggcaaacc cgtggaagat aatcaaacat atcgtattgc
     1381 cacgcactcc ttttgtgcca gtggtggtga cggttttgaa gcatttttga atggaaaaaa
     1441 tgtgaagacg ataccgggaa caacctcggc ggaatctatc atcgattatt tcaaaaatca
     1501 taaacctgtc accccagact taactaaacg agtcatggac gtcgccaaat aaaatctcat
     1561 ttatcagtca atttatttaa caaggaatgc gaatgaaaac agcgcagcgt actttatttg
     1621 ccgcaacatt gctgactatt ttttcgacct cagttatggc gcaggatgtc acgattattt
     1681 ataccaatga tcttcacgct catgtggatt cctataaagt accgtatgtc gcagacggaa
     1741 aacgcgacat tggtggcttt gccaatatat ccacgttagt aaaacaagag aaagccaaaa
     1801 ataaagccac cttttatttt gatgctggag actatttcac cgggccatat atcagtagcc
     1861 tgacaaaggg cgaagcaata attgatatta tgaataccat gccttttgac gcagtatcaa
     1921 tcggtaatca tgagtttgac catggctggg ataatgccct ccgacaatta agcaaagcga
     1981 atttccctgt tttattagcc aatgtttatc ataaagagag tgagaaaccc ttctggaaca
     2041 agccctacac catcctggaa aaagatggca tcaagattgg tattgttggg ctacacggcg
     2101 tatttgcatt taatgacacg gtctcggagc tctctctcca gggactcgat aatgacaata
     2161 ataaccgatt tgataaatct tccgcgaccc tcaagaatca ggggattgaa gcacgtgatg
     2221 aagtgaagta tttgcagcat tatatcgatg agttacgtga taaggttgac ctcaccgtgg
     2281 cgctggttca tgaaggcgta ccggcgcgtc agtccagcat tggcaataca gatgttagac
     2341 gcgctcttga taaagatatt caaaccgcaa gcaaggtgaa aggattggat attctgatta
     2401 ccgggcatgc ccacgtcgga acgccagaac caatcaaagt cggcaacacc ttaatccttt
     2461 cgactgacag tggcggtatc gatattggta aattaatcct tgatgtcaac cctacagctc
     2521 gtactcataa agtgaagagc tttgagctga aaacggttta tgccgatgaa tggatacctg
     2581 atccaaccac acaaaaagtt atcaatggct ggaataaaaa actggcggat cttgtacgcc
     2641 aaccagtcgg tgaatcatcg attgcattaa ctcgtgccta cggtgagtca tcgcaacttg
     2701 gcaacctgtt taccgatgca atgcttgtcg ctgcaccgac tgcacagatt gcattgataa
     2761 actccggcag cttacgtgcg gatataaatg caggcacgat tacctttggc gacattacca
     2821 gtacattccc cttcaagaat gaacttactg aaatggatct cagcggtaag gatctgcgta
     2881 acctactgga acacggggca tcgcttacca atggcatcct gcaaatgtca aaaggcgcag
     2941 aaatgcgcta taccccgaag aaaccagtag gtcagcgcat agtatctttt aaaattaatg
     3001 gtgaagagat cgttgatacg aatatttacc atgttgcaac aacgactttc cttgcactgg
     3061 ggggcgatgg ttttctggca ttcaaagaag ggaaaaatgt tcaggtccgc gccggaaata
     3121 acatgtccga tgtcgtgatc gattatttga agaaaggcca caagattac
//
LOCUS       DAZUCC010000348         3157 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZUCC010000348 DAZUCC010000000
VERSION     DAZUCC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.350"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..1651
                     /gene="ydiF"
                     /locus_tag="ACHY09_004959"
     CDS             56..1651
                     /gene="ydiF"
                     /locus_tag="ACHY09_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFS3887788.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            1665..2816
                     /gene="ydiO"
                     /locus_tag="ACHY09_004960"
     CDS             1665..2816
                     /gene="ydiO"
                     /locus_tag="ACHY09_004960"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFS3887789.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(2859..>3157)
                     /locus_tag="ACHY09_004961"
     CDS             complement(2859..>3157)
                     /locus_tag="ACHY09_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3887790.1"
                     /translation="FRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQA
                     EISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
BASE COUNT          853 a          731 c          808 g          765 t
ORIGIN      
        1 tgcgctaatt ctccatctgg cgtagggaaa atcacacctg aatcaggaat taacaatgaa
       61 acctgtaaaa ccacctcgca ttaatggacg agtgccggtc ctgtcggcac aggaagcggt
      121 gaattatatt cccgacgaag caacactttg tgtgttaggc gctggcggcg gtattctgga
      181 agccaccacg ttaattactg ctcttgctga taaatataaa cagactcaaa caccacgtaa
      241 tttatcgatt attagtccaa cagggcttgg cgatcgcgcc gaccgtggta ttagtccgct
      301 ggcgcaagaa ggcctggtga aatgggcatt atgcggtcac tggggacaat cgccgcgtat
      361 ttctgatctc gcagaacaaa ataaaattat tgcttataac tatccacaag gtgtacttac
      421 acaaacctta cgcgccgccg cagcccacca gcctggtatt attagtgata ttggcatcgg
      481 aacatttgtc gatccacgcc agcaaggcgg caaactgaat gaagtcacta aagaagacct
      541 gattaaactg gtcgagtttg ataacaaaga atatctctat tacaaagcga tagcgccaga
      601 tatcgccttc attcgcgcta ccacctgcga cagcgaaggc tacgccactt ttgaagatga
      661 ggtgatgtat ctcgacgcat tggttattgc tcaggcggtg cacaataacg gcggtattgt
      721 gatgatgcag gtgcagaaaa tggttaagaa agccacgctg catcctaaat ctgtccgtat
      781 tccgggttat ctggtggata ttgtggtggt cgatccggat caaacccaac tgtatggcgg
      841 agcgccggtt aaccgcttta tttctggtga cttcaccctt gatgacagca ccaaacttag
      901 cctgccccta aaccaacgta aattagttgc acggcgtgca ttattcgaaa tgcgcaaagg
      961 cgcagtgggg aatgtcggcg tcggtattgc tgacggcatt ggcctggtcg cccgggaaga
     1021 aggttgtgct gatgacttta ttctgacggt agaaacaggt ccgattggcg gtattacttc
     1081 acaggggatc gcctttggcg cgaacgtgaa tacccgcgcc attctggata tgacgtccca
     1141 gtttgatttt tatcacggtg gtggtctgga tgtttgttat ttgagttttg ctgaagtcga
     1201 ccagcacggt aacgtcggcg tgcataaatt caatggtaaa atcatgggca ccggtggatt
     1261 tattgatatc agtgccactt cgaagaaaat cgttttctgc ggcacattaa ctgcgggcag
     1321 tttaaaaaca gaaattaccg acggcaaatt aaatatcgtc caggaaggac gggtgaagaa
     1381 atttattcgg gaactaccag aaattacttt tagcggaaaa atcgcgctcg agcgagggct
     1441 ggatgttcgt tatatcactg agcgcgccgt attcacgctg aaagaagacg gcctgcattt
     1501 aatcgaaatc gctcctggcg tcgatttaca aaaagatatt ctcgacaaaa tggatttcac
     1561 cccagtgatt tcgccagaac tcaaactgat ggacgaaaga ttatttatcg atgcggcgat
     1621 gggttttgtc ctgcctgaag cggctcatta acaggagtat aatgatggat ttttctttaa
     1681 ctgaagaaca agaactgctg ctggccagta ttcgcgaact gattacgact aactttccgg
     1741 aagagtattt ccgcacctgc gatcaaaacg ggacataccc gcgtgaattt atgcgggcac
     1801 tggcggataa cggtatttcc atgcttggcg tgccggaaga atttggtggt atccctgcgg
     1861 attacgtcac ccaaatgctg gcgctgatgg aagtgtcaaa atgcggtgcc ccggcatttt
     1921 tgatcaccaa cggtcaatgt attcacagta tgcgccgttt cggttctgca gagcagctac
     1981 gtaaaacggc ggaaagcacc ctggaaacgg gtgatcccgc ctatgccctg gcattgacag
     2041 agccaggcgc aggctcagac aacaacagtg ccactaccac ttacacgcgt aaaaacggca
     2101 aggtttacat caacggacag aaaaccttta ttaccggtgc gaaagagtat ccatatatgc
     2161 tggtgttggc gcgcgatccg caaccgaaag atccgaaaaa ggcctttact ctgtggtggg
     2221 tcgactccag taagcccggc attaaaatca atccactgca taaaatcggc tggcatatgc
     2281 tcagcacctg cgaagtctat ctcgacaacg tggaagttga agagagcgac atggtgggcg
     2341 aagaaggaat gggtttcctc aatgtgatgt acaactttga gatggagcgc ctgatcaacg
     2401 ccgcgcgcag caccggcttt gccgaatgcg cattcgaaga tgccgcccgc tatgccaacc
     2461 agcgtatcgc ttttggtaag cccattggtc ataaccagat gatccaggaa aaactggcgc
     2521 tgatggcgat taagatcgac aacatgcgca acatggtgtt gaaagtggca tggcaagccg
     2581 atcagcatca gtcactgcgc accagcgcgg cgctggcaaa actgtactgc gcacgtaccg
     2641 caatggaagt cattgatgat gcgattcaaa tcatgggcgg tctgggctat accgatgagg
     2701 cgcgcgtctc ccgcttctgg cgtgatgtcc gttgtgaacg tatcggcggc ggtacagacg
     2761 aaattatgat ttacgtagca ggtcggcaga tcctgaaaga ttatcagaac aaataatctg
     2821 caggcggcgc agcttcttaa caaactgcgc cgccagattt atccggcaag acttactggt
     2881 tgaggaaatt cagataggat ttcttgttcc gcaaaacagt ttttaaattg cctgcggtaa
     2941 tcgctgggcg aacagccgac atggcgcaaa aacagtttgg caaagtgatc gacattttca
     3001 taacccacac gccaggagat ctctgcctgt gataattcag tattagtgag tgaccatttc
     3061 gcttccgtca tacgccgttg aataacatag ttaatagggg aaatgcgata ctctttggta
     3121 aattcatggc aaatataact gacgctggca cgaaact
//
LOCUS       DAZUCC010000349         3133 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZUCC010000349 DAZUCC010000000
VERSION     DAZUCC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.351"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..315)
                     /locus_tag="ACHY09_004962"
                     /pseudo
     CDS             complement(5..315)
                     /locus_tag="ACHY09_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(711..1520)
                     /gene="iolB"
                     /locus_tag="ACHY09_004963"
     CDS             complement(711..1520)
                     /gene="iolB"
                     /locus_tag="ACHY09_004963"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFS3887791.1"
                     /translation="MTNLLSTCTTRAGNIQHISPQNAGWEYVGFDVWLLKAGESLTLP
                     SDDRERCLVLVAGLASVKAADHFFYRIGQRMSPFERIPAYSVYLPHHTEAKVTAETDL
                     ELAVCSAPGFGELPVRLISPQDIGVEHRGKGRNQRLVHNILPDTQPADSLLVVEVYTR
                     EGDTSSWPAHKHDTAVEGQETYLEETYYHRFDPPQGFSLQRVYTADRSLDECMAVYNR
                     DVVKVPKGYHPVATIAGYDNYYLNVMAGPLRKWQFTWEADHAWINSSEYPR"
     gene            complement(1545..3050)
                     /gene="iolA"
                     /locus_tag="ACHY09_004964"
     CDS             complement(1545..3050)
                     /gene="iolA"
                     /locus_tag="ACHY09_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate-semialdehyde dehydrogenase IolA"
                     /protein_id="HFS3887792.1"
                     /translation="METVGNFIQGKTRLSNSGETLPVTNPATGEVIRLVTQSTRQDML
                     EAIQSAHEAFPAWSKMAPLRRARILFEFKVLLEKHRDELAALIVSEHGKVWSDALGEL
                     TRGIEVVEFACGIPHLIKGEFSFNVGTGVDSFSLMQPLGVVAGITPFNFPAMVPMWMF
                     PIALACGNTFVLKPPALVPSASLRMAQLLQEAGLPDGVFNVVHCGNDAASLLTSDPRI
                     QAVSFVGSSSVAEHIYTTASAHGKRVQAFGAAKNHAIVMPDADLDATVNAIMGGAFGS
                     AGERCMALPVVVAVGDETANRLIERLKPLIAALRIGPGELREKDENEMGPVVSRMHQQ
                     KVLAYIDKGVSEGATLVMDGRNYCVKGYPEGFYVGGTLFDNVTPEMTIWREEIFGPVL
                     GIVRVTDYATAIRTVNSHEFGNGSVIFTSNGHYAREFAQSVEAGMVGINVPVPVPMAF
                     HSFGGWKRSVFGALNVHGPDGVRFYTRMKTVTARWPNGQQIASEFSMPTLG"
BASE COUNT          818 a          786 c          727 g          802 t
ORIGIN      
        1 tcggctatat tatctttttc atgttggtgc ttttattctg tgaggtgtgc tggcatggtg
       61 gacacaacca tttgttcttt ttagctgcca tttgataata ttttcctgaa gctttccggg
      121 gatatattcc ccagacggga gtggcggcgc tgacggttgt agaatatctc aatgtatacc
      181 cagtattact gagatgactt cattccggct actaaaatgg tagtggttca gactttcatt
      241 cttcagagtt ccccaaaagc tttccatagg ggttttgtcg taacagttcc ctttacgaga
      301 cattgatgtt ttcagagcta actgcgatag actccttcaa cttcaacttc aacttcaact
      361 tcaacttcaa cttcaacttc aacttcaact tcaactgaga aacggatgat ttcacgatag
      421 actgcccaac gtattgagcc cgttaggaag ccagagtaac aagccgccgt gtacggcatt
      481 tctctcctaa ttcggcagaa ccaaaaatat tgtcagctca ggagtaagaa tcagaaaaaa
      541 ccataacaga acaccagaaa caggatatta tcagacagag aaaaaattca aaaaaatcca
      601 ccgattctgt tgcctgtgga tttgtgtata agaggcagaa ccctgttggg gactgtgcac
      661 aaaaaagcca gctgcagagg acaactggca aaggaatatt taacgtattg ttaacgcgga
      721 tactctgacg aattaatcca tgcgtgatca gcttcccatg tgaattgcca tttgcgtaat
      781 ggcccggcca tcacattcag ataatagtta tcgtaacctg cgatagtggc caccggatga
      841 tagcctttcg ggacttttac cacatcgcgg ttatatactg ccatacattc gtccagactg
      901 cgatcggcgg tgtagactcg ctgtaagctg aatccctggg gtggatcgaa acggtggtag
      961 taggtttctt ccagataggt ttcctgccct tcaactgctg tgtcatgctt atgggcaggc
     1021 caggaacttg tatcgccttc ccttgtatat acttccacta ccagcagact gtcagcaggt
     1081 tgagtgtctg ggaggatatt atgaaccaag cgctgattgc gcccttttcc cctatgttcc
     1141 accccaatat cttgtggtga gatcagtctt accggaagtt caccgaatcc aggagcactg
     1201 cagacagcca gttccagatc agtttcggca gtgacttttg cttctgtatg gtgcgggagg
     1261 taaacagaat aagccggaat tcgctcaaaa ggactcatgc gctggccaat ccgataaaaa
     1321 aagtggtcag cggcctttac gctggcaagt ccggcaacca gaaccaggca acgttcgcga
     1381 tcatcggatg gcaaggttaa agattcgccg gctttcaaca gccagacatc aaaaccgaca
     1441 tactcccagc ctgcgttttg cggagaaata tgttgaatat ttccagccct ggtggtgcag
     1501 gtactcagca agttggtcat aatcttctcc ttcctgaacg tttattaacc tagcgtaggc
     1561 atactgaact cggatgcgat ctgctgaccg ttcggccagc gtgcggtaac ggttttcatc
     1621 cgggtataaa aacgcacccc atccggtccg tggacattca gcgccccaaa caccgagcgt
     1681 ttccagccgc cgaaagagtg aaacgccatt ggaaccggta ccggaacatt gataccgacc
     1741 atcccggctt caaccgattg agcaaattcg cgggcataat ggccgttgga ggtgaagatc
     1801 acgctaccat tgccaaattc atgagagttt accgtacgaa tggcggtggc ataatctgtt
     1861 acgcgtacga tgccgagcac gggaccaaaa atttcctcac gccagatagt catttccggc
     1921 gtgacgttat caaacaaggt accgccgaca taaaatcctt caggatagcc cttcacgcaa
     1981 taatttcgcc catccatcac cagtgttgct ccttcgctaa cgcctttgtc tatatatgca
     2041 aggacttttt gttgatgcat acgtgaaaca actggcccca tttcattttc gtctttttct
     2101 cgtaactcac cggggccaat acgcagcgct gcaataagtg gttttaggcg ttcaataaga
     2161 cggttggctg tttcatcacc gacagcgacc accacaggta atgccataca acgttcgcct
     2221 gcggaaccaa aagccccacc cataatagcg ttaacggtag catcaaggtc agcatccggc
     2281 atgacaatag cgtggttttt tgcggcaccg aaagcttgga ctcgcttacc gtgtgcactg
     2341 gcggtggtgt agatatgctc agcgacggaa gaagatccga caaaactgac ggcctgaatg
     2401 cgtggatcac tggtcaacag gctggctgcg tcattgccac agtgaactac attgaataca
     2461 ccatccggta gtccggcctc ctgtagtagt tgcgccatac gtaacgatgc ggagggcacc
     2521 agcgctggag gcttcaggac gaaggtattg ccgcaggcga gagcgatagg gaacatccac
     2581 attggcacca ttgcagggaa gttaaagggt gtgatgccag ctacaacacc aagtggttgc
     2641 attaatgaaa agctgtcaac gcctgtgcca acattaaaag agaactctcc tttaatgaga
     2701 tgcggaatgc cgcaggcgaa ttcaacaact tcgattccgc gagtcagttc accaagcgcg
     2761 tcggaccata ctttgccatg ttcactgacg atcagagccg ccagttcatc tcggtgtttt
     2821 tccagcaaca ctttaaattc aaaaaggatt cttgcacggc gcaacggtgc cattttactc
     2881 catgcaggaa aagcttcatg agcactctga atcgcctcca gcatatcttg ccgggtgctc
     2941 tgggtaacca ggcgaatcac ttctccggtt gcagggttag tcaccggtag tgtttcacca
     3001 ctgttgctca gtctcgtttt accctgaata aaatttccaa ctgtttccat cactgtttac
     3061 cttcttgaat caatgttaac gacgttccga ctgagtgttc acaggcaaag cctgatcatc
     3121 tacagtctaa aat
//
LOCUS       DAZUCC010000350         3123 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZUCC010000350 DAZUCC010000000
VERSION     DAZUCC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.352"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..308
                     /locus_tag="ACHY09_004965"
     CDS             <1..308
                     /locus_tag="ACHY09_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887793.1"
                     /translation="LNEHGTTDRAVVYNGKHGFMTIYPGPIRFALQQYIEWNLIQKYG
                     EAEGMGRALFLYQKMLITCPDKGFILSGMGREGLELLLNEMINEMNTHSMQSETAIN"
     gene            319..573
                     /locus_tag="ACHY09_004966"
     CDS             319..573
                     /locus_tag="ACHY09_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS3887794.1"
                     /translation="MTVIEYIRENPDCSRQDIALALGKSTVSVGNQLSLLLWKGLIVK
                     TGAENKMILYRVNNLPFGYSNPLSVMFNQLLKQVRKSDGN"
     gene            563..829
                     /locus_tag="ACHY09_004967"
     CDS             563..829
                     /locus_tag="ACHY09_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFS3887795.1"
                     /translation="MATDSQLTVDTALNVGLALLGYFYIMFCAGRWLSLLFMKKWNKR
                     RKQEQRQKAMNAFSEAFGIDSMEPGDPARVITRGDIVILVYRSE"
     gene            834..1322
                     /locus_tag="ACHY09_004968"
     CDS             834..1322
                     /locus_tag="ACHY09_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS3887796.1"
                     /translation="MSKIDYQALRKAAQLATQGEWVAFISPGKYGTYAVHTPGDNHHG
                     DIVDWPGFDEQKNAENNARYIAAFNPEVVQALLDERERNQQYIKRRDQENEEIALTVG
                     KLRVELEAAQKHIAELDAIKSAAEKLVRCKGRYHSEQNYRALAALFGVTVPDLPPLEN
                     DK"
     gene            1365..1606
                     /locus_tag="ACHY09_004969"
                     /pseudo
     CDS             1365..1606
                     /locus_tag="ACHY09_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            1610..1744
                     /locus_tag="ACHY09_004970"
     CDS             1610..1744
                     /locus_tag="ACHY09_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887797.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCRDVVKRMFDEWFHCRG
                     "
     gene            1763..1978
                     /locus_tag="ACHY09_004971"
     CDS             1763..1978
                     /locus_tag="ACHY09_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS3887798.1"
                     /translation="MALNIDCVPITTWCRDMGETLDAVNKRLQRGVWKEGVQVLNVEG
                     SKERWIDLTEVSKWARKNKDPYHSQEE"
     gene            1933..3105
                     /locus_tag="ACHY09_004972"
     CDS             1933..3105
                     /locus_tag="ACHY09_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3887799.1"
                     /translation="MGEKKQRSLPLPRGITIRQYKNCSTLVVTFTYKGVLCREPLPRL
                     EANAKGIKYAERLLGEIKNQIVDGSFDFSKHFPNSKKLALFGMVRKNKTVRDYLDEYL
                     EICKNRKLSPSTINGYEKCVSALSELHKLHVVDLTPAILKKWISRRTTKLKTIRNNLS
                     FLRSAVDEAVTDGLITINPVKLVSASRYHAVNHAINSGDYEVDPFTPAEASAIYNACI
                     YEEWRNLFCFAFNTGLRSSELCALRWSDIDFIGHTVHVQVASVVGVIKGTKTKAGTRK
                     VELNSEAIMALQAQKSFTMMRSDFVFSDPKTRKPWANSAAIRKKAWIPTLKKAGVRYR
                     NPYQTRHTFATRHISQGANLFWLAGQMGHKGPEMLFRHYGSYLSEYDGKTAISEVI"
BASE COUNT          991 a          646 c          724 g          762 t
ORIGIN      
        1 tcctgaatga gcacggaaca acagatcgcg ccgtagtata taacgggaag catgggttta
       61 tgacgatata tcccggtccc attcgttttg ccttacaaca gtatattgaa tggaatttaa
      121 ttcaaaaata cggcgaggca gaaggtatgg gtagagcgct gtttctttat cagaaaatgc
      181 tcattacatg cccagataaa ggtttcattc tttcaggtat gggccgagaa ggactcgaac
      241 tccttctgaa tgaaatgatt aacgaaatga atactcacag catgcaatca gaaaccgcta
      301 ttaactaaaa ggaatccaat gaccgttatt gaatatatcc gggaaaatcc ggattgcagt
      361 cgtcaggata tagctctcgc tcttggaaaa agtaccgttt ccgtcggtaa tcaattatca
      421 ctgctattgt ggaaaggatt aattgtaaaa accggagcag aaaacaaaat gattctgtat
      481 cgcgtaaaca atctgccgtt tggatacagc aatcccctga gtgttatgtt caaccaatta
      541 cttaaacagg taagaaagtc tgatggcaac tgactctcaa ctaaccgtag atacggctct
      601 taatgtcggc ctggcgctcc ttggttattt ctacatcatg ttctgcgccg gacggtggct
      661 gtcactgttg ttcatgaaaa aatggaataa acgccgtaag caggagcaac gccagaaggc
      721 aatgaatgcc ttttccgaag ccttcgggat tgacagtatg gagccaggag atccagcacg
      781 cgtcatcacc agaggtgaca ttgtaattct tgtatatcgg agtgaataga aaaatgagca
      841 aaattgacta tcaggcactg cgtaaagcgg cgcaactggc aacacagggc gaatgggtcg
      901 cattcatttc gccgggcaaa tacggcacat acgccgtaca cacaccagga gataatcatc
      961 acggagatat tgtcgactgg cctggattcg acgaacagaa aaacgcagag aacaacgctc
     1021 gttatatcgc agctttcaac cctgaagtag tgcaggcgct gctggatgaa cgggaaagaa
     1081 accagcaata catcaaacgc cgcgaccagg agaacgagga aattgctcta acggtaggga
     1141 agctgcgtgt tgagctggaa gccgcacaga agcacatagc agaactggat gcaatcaaat
     1201 ccgctgcgga aaaactggtt cgctgtaaag gccgctatca cagtgagcaa aactatcgtg
     1261 cgctggctgc attgtttggc gtaacagtgc cggatttgcc accactggag aacgataaat
     1321 gagctggcct gatgcatttg caatttacca ctggggataa ctgtatgttc gcacttattc
     1381 aacgcggaca aatatacacg gacagggccg gataccctgt agtgattact cgctgtacgg
     1441 atctctctgt gttctttcga cgtatggacg gacactccgg tcgggtacgc attggtgagt
     1501 tcaccgcctg ttcgaacata ttgaccacca gcagaattgc aaaattctgg cggaaactga
     1561 gcaggaaaag cacctgaaaa aattacgcgc aatgcaacgg aggtgataca tgcatacggc
     1621 ttttgagttc tgggttcgca agacattcgg caatcgctac gacctgaccc gtgatgttga
     1681 cggcttctac tgccgtgacg ttgtgaaacg aatgtttgac gagtggttcc actgccgtgg
     1741 atgaaggttt tatgaggtaa ctatggctct caacattgat tgtgtaccca taacgacatg
     1801 gtgtcgagat atgggagaaa cgctggatgc cgttaacaaa cggttacaac gtggagtatg
     1861 gaaggaaggg gttcaggttt taaatgtgga aggctccaaa gaacgatgga ttgatttaac
     1921 tgaggtttca aaatgggcga gaaaaaacaa agatccttac cactcccaag aggaataaca
     1981 attcgtcaat acaagaattg ctctacacta gttgtaacct tcacatataa aggggttctt
     2041 tgcagagaac ccttaccaag actggaggct aatgcaaagg gaattaagta cgctgagcga
     2101 cttctcggcg aaataaaaaa tcagattgtc gatggctctt ttgatttctc aaaacatttt
     2161 ccaaactcaa aaaaattagc attatttgga atggtaagga aaaataaaac agtaagagat
     2221 taccttgatg aatatctgga aatttgtaaa aacagaaaac tctccccttc aacaataaat
     2281 gggtatgaaa aatgcgtatc cgcattatcg gaactacata agctacatgt tgtagatctg
     2341 acaccagcaa tattaaaaaa atggatttca agaagaacga caaaactgaa gaccataaga
     2401 aacaatctgt catttttaag aagcgcagtt gatgaagcag tcactgacgg acttataaca
     2461 ataaatcctg taaagctggt tagcgcaagt agataccatg cggtaaatca tgcaatcaat
     2521 tctggcgatt atgaagtaga tcccttcacg ccagcagaag catccgctat ctacaacgcc
     2581 tgcatctatg aggaatggag aaaccttttt tgtttcgcat tcaatactgg ccttcgaagc
     2641 tcagaattgt gtgcattacg ctggtctgat attgatttta ttggtcatac cgttcacgta
     2701 caggttgcct ctgtcgttgg cgtgataaag ggaacaaaaa caaaagctgg taccagaaaa
     2761 gttgaattaa acagtgaagc cataatggca ttacaggccc agaaatcatt tacgatgatg
     2821 agaagtgatt ttgtattcag tgatccaaag acacgcaagc cgtgggctaa ttcagcagcg
     2881 attcgcaaga aagcctggat cccaacactt aaaaaagctg gcgttcgtta ccgcaatcca
     2941 tatcaaacca gacacacctt cgccacgagg catatcagcc aaggggcaaa tttattctgg
     3001 cttgccggtc aaatgggaca taaaggacct gagatgcttt ttagacacta tggttcatat
     3061 ttatcagaat atgacggaaa aacagcaatc tcagaagtaa tctagccgtt taaatatttc
     3121 aaa
//
LOCUS       DAZUCC010000351         3112 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZUCC010000351 DAZUCC010000000
VERSION     DAZUCC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.353"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1698)
                     /locus_tag="ACHY09_004973"
     CDS             complement(<1..1698)
                     /locus_tag="ACHY09_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887800.1"
                     /translation="MMNIRYPVRKADGRDYKSYDELLTDIRKNAHGWWLLGVNRYWHG
                     GIHVGRSSSPASVLNQETPEKSVPLQFMMDGEVVAWRVNRDYAAIECYQERPLRQSGT
                     FVLVKSVYKPDEQDESSWLTLYQLYMHIAPLSEFPKRPLYRVTQKGHGVRMRKYSRHD
                     DSREIAPDVLANKHGHPRTLTQGDTLAVLQQKSFLLKQRPESFALVQRLQDGKPAGEL
                     FWVSMRPEYLEPDGQCYVCLPDWMHSALNHGVFDDVVVPPVPLKVMVKAGDAVGFLGA
                     QDLADEDNYPQIITTDYKAHIELLSLDEHVPDVVANMKGIKTGKQFIKLKLKRPLYLR
                     NGEGEESTFEQMSAITRIDAGKIIPRDATYPFTDKTGITYFQIRPHTWMHQNDVEQLS
                     QHDLAGLNFHCIEAEHTTDFTRTLDERWLIDALKSISSHFDGEKGPQFAQAKMFYDSL
                     IHNAENRRAPNPYPDKSLDQYLFGALHTNQMNIPEYARRLIVKHDSCWHSTRDDTRWS
                     SVCKSRDESPVVKMANGGFLDATHWMDKVPPFASQRSVWHFHPLEFPEILRSAEFPRT
                     "
     gene            complement(2039..2253)
                     /locus_tag="ACHY09_004974"
                     /pseudo
     CDS             complement(2039..2253)
                     /locus_tag="ACHY09_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742560.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2259..2945)
                     /locus_tag="ACHY09_004975"
     CDS             complement(2259..2945)
                     /locus_tag="ACHY09_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFS3887801.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSVASMMAQMQLGNVKALFESKPFITLSEPACGAGSMILAMVDTLNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
BASE COUNT          756 a          812 c          721 g          823 t
ORIGIN      
        1 agttctggga aactctgcac ttcgtaatat ttccggaaac tcaagcggat gaaaatgcca
       61 tacagaccgc tgactggcga acggaggcac cttatccatc cagtgtgttg catccagaaa
      121 tcccccgtta gccatcttca caaccgggct ttcatccctg gacttacata ccgatgacca
      181 gcgtgtatca tcgcgggtac tgtgccagca gctgtcgtgt ttcactatca ggcgtcgcgc
      241 atattccggg atgttcatct gattggtatg cagcgcacca aacaaatact ggtccagaga
      301 tttatcggga tacgggtttg gcgctcttct gttttcagcg ttgtggatca ggctgtcata
      361 aaacattttt gcctgcgcga actgcggtcc tttttcaccg tcaaagtgcg agctgatgct
      421 tttcagggca tcaatgagcc agcgttcatc cagcgtgcgg gtaaaatctg ttgtgtgttc
      481 agcttcaata cagtgaaaat ttagccctgc taaatcgtgc tgactgagct gctccacatc
      541 gttctggtgc atccacgtat gtgggcgaat ctgaaagtat gtgatcccgg ttttatccgt
      601 aaacgggtac gtggcatcac gtgggataat ttttccggca tcaattcggg ttatggcgct
      661 catttgttca aatgtacttt cttcaccctc tccatttcgc aggtacagcg ggcgtttcag
      721 cttcagctta atgaactgtt tgcctgtttt aatccctttc atgtttgcca ccacatctgg
      781 cacatgctca tcgagactca gtagctcgat atgggcttta tagtccgtgg taataatttg
      841 cggatagtta tcttcgtcag ccagatcctg agcgccgagg aatccgaccg catcccctgc
      901 tttcaccatc actttcagtg gcacaggcgg caccaccaca tcatcaaaca cgccgtgatt
      961 cagcgcactg tgcatccagt caggcaggca cacgtaacat tggccatcag gttccagata
     1021 ttccggacgc atcgagaccc agaaaagttc tccggcgggt ttaccgtcct gaagacgttg
     1081 caccagagca aacgactccg gccgctgttt cagtaaaaac gatttttgtt gcagtacggc
     1141 aagcgtgtca ccctgcgtca gtgttctggg gtggccgtgt ttgtttgcca gtacgtccgg
     1201 cgcgatttca cggctgtcat catggcggga atatttacgc atccgcacac cgtggccttt
     1261 ttgtgtaacc cggtaaagcg ggcgtttcgg aaattctgac aatggcgcga tgtgcatgta
     1321 aagctggtac agcgtcagcc aggatgattc atcctgctcg tctggcttgt acactgattt
     1381 taccagcaca aaggtaccgg actggcgcag ggggcgttcc tgataacatt cgatagccgc
     1441 gtaatcgcgg ttaacccgcc atgccaccac ttcgccatcc atcatgaact ggagcggcac
     1501 cgatttttca ggtgtttcct gattcagcac cgaggccggg gacgatgacc tcccgacatg
     1561 aatacctccg tgccagtaac gatttacgcc aagtagccac caaccgtggg cgtttttgcg
     1621 gatgtcggtt agcagctcgt catagctctt atagtctcgt ccatcggctt tccgtaccgg
     1681 ataacgtatg ttcatcatct tttcttaagt gaaatcacat atttcgatgg attctcgcgc
     1741 gtattttggg tttggggtgt agtggaacca aaaagacggt taagcggtta aattgaatgg
     1801 tcaataacag gtaaatcaat ctgttgagat caacgacgtc ttttgctccg caaacggcta
     1861 agatgagtac agataatttg gttatcgtaa aaaaggtggc taaaaatttc aacctgcaat
     1921 gtaaattggc cggttgattt ttggctattt aaattgacct cttaacggcg tttattgttc
     1981 cattgctccc caacccccta tggattataa gccgcacata cattacttcg ttctgcatat
     2041 caccactaat ctgcttaaga tgtactaaca acatcatcgc ctgttggcta aacggaacta
     2101 taactcctct ttcattttca tgccgcgata caagtaatgc acacctttaa tttcttcacg
     2161 ttttgccgga atgcgccaaa tagcgttatc aaaatcaaat tctttttaac gagcaaaacg
     2221 ccagtccgaa gactggcgca gtgataaaac attctgcatc atgccacatt ctgctcctga
     2281 agatgacgca gacgatggtc ccagtttccc aggtaatgcc cgggagtcag cagtacgcgg
     2341 cgtcgctcat tacagagcga gttaccaatc accacttctc cggccacacc acacagcgac
     2401 agttgtatat acgccatgcc ggctgccagc gggtcaatat ccgttgccga cacccacatc
     2461 ctgtggtatg ccggataccc cgaacggttc agggtgtcaa ccattgccag aatcatactg
     2521 ccggcaccac aggcaggttc actgagcgtg ataaagggtt tactctcaaa caacgccttc
     2581 acatttccca gttgcatctg tgccatcatg ctggcaacac tccagggcgt gaaaaactga
     2641 ctccggtatt tatcccccag ttccagttgc atgaacacac tacccagaaa atcacagaat
     2701 ccctgctcca gtccctcaat gacatgagca agcaaatgcg caatgtggat aacatcctgc
     2761 ttttcataac cactgacgat tcgcagatac ttctgttccc gggcctcact gaactgaagc
     2821 cggttttcca gggcaatcac actacagctg acaaagtctt caaacacctt atgacgtttg
     2881 tgataacagg ctgtccggtt aaacaacgaa atgaattcct tctgatggtc agtcatggtg
     2941 gccatgtttt ctccgtaaaa acagggaaca catggcccgg acaggggaac aggtgttccc
     3001 cttcgggttg aaatgttgat aaaacgggca tcacggacat aatacccgga ggttaattac
     3061 tgggctgaga ccagaggaac cggtaaaggt gcggctttcg tctgttcttc ag
//
LOCUS       DAZUCC010000352         3098 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZUCC010000352 DAZUCC010000000
VERSION     DAZUCC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.354"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..299
                     /locus_tag="ACHY09_004976"
     CDS             <1..299
                     /locus_tag="ACHY09_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS3887802.1"
                     /translation="WSRINELLTEPDIIAFINYPIAHSLMHLNDDEIHGVRPEEFPDE
                     EMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDDIDDFSDKVIQKAMMLRT"
     gene            301..726
                     /gene="nudI"
                     /locus_tag="ACHY09_004977"
     CDS             301..726
                     /gene="nudI"
                     /locus_tag="ACHY09_004977"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFS3887803.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            complement(1090..1548)
                     /locus_tag="ACHY09_004978"
     CDS             complement(1090..1548)
                     /locus_tag="ACHY09_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887804.1"
                     /translation="MRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQRASRLATSV
                     KRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHR
                     SADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG"
     gene            complement(1689..2537)
                     /locus_tag="ACHY09_004979"
     CDS             complement(1689..2537)
                     /locus_tag="ACHY09_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS3887805.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQLLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGGRPVIIFNARFDIRILKQTAAAHSDPAYWLEEMTVYCAMEL
                     AAGYYGATNRYGTISLASAASQAGLTWEGLAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLEI"
     gene            complement(2583..2861)
                     /gene="yacB"
                     /locus_tag="ACHY09_004980"
     CDS             complement(2583..2861)
                     /gene="yacB"
                     /locus_tag="ACHY09_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFS3887806.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(2861..>3098)
                     /gene="yacA"
                     /locus_tag="ACHY09_004981"
     CDS             complement(2861..>3098)
                     /gene="yacA"
                     /locus_tag="ACHY09_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFS3887807.1"
                     /translation="AELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAA
                     GRQQLEHGDVLPHDMVESSAAAWRDKMSRKVAGK"
BASE COUNT          778 a          809 c          721 g          790 t
ORIGIN      
        1 tctggagtag aataaatgaa ttacttacag agccagacat tatagcattc attaattatc
       61 ccatcgcaca ttcactcatg catctcaatg atgatgaaat tcatggtgta cggcctgaag
      121 aatttccaga tgaggaaatg aagataaaat ggatcacagg atggcatgcg gaatatcaac
      181 attttactga taatcttccg ttacaccttc gtgagcggat tatggtatgt gctcacccgg
      241 atgacattga tgatttttct gataaggtta tccaaaaagc catgatgttg aggacataaa
      301 atgcgccaga gagtaatagt gtgccctgtg atacaaaatg aaggtgagtt tctactttgt
      361 aaaatggcct ctgatcgcgg agtattcccg ggccaatggg cactgcccgg tggaggaatg
      421 gaacccggag agactatgga aacagcattg aggcgtgaaa taaaggaaga actgggaaaa
      481 gaactgcata tcactgacgt aaaaccctgg gcctttcgtg atgatatccg tacaaaaacg
      541 tacccggatg gtacccacga ggacatttac atgatttacc taatattcga ctgtatcagt
      601 acaaaccgta cagttacatt taatgaggag tttcaggata tagtctgggc atctcctgac
      661 accctaaaat atatggattt aaatgaagca acccggatca cattttctca gaaaggtatc
      721 ctttaaactc caatgctaat cgtgcaactg ataacggctt ccacaacagg gagccgtttt
      781 cttatgccac tactcaagga tggggtttga gggccaaccg tacgaaaacg tacaattctg
      841 tacatctacg caaggaaatg tacggaagta agccaaatgg ttatcggacg gtcggaacat
      901 ttcgtacagt ctttacgttc agtgagttag ctggcctgga tgtaaccact cgttcaactc
      961 aattctctta gcgtgggaaa atttccgaat tccaagcgga ccccttattg tacgttcaac
     1021 gcaccatcat taatgctaat gtatgcatac attagcatta atgatgaagg agaagaataa
     1081 caatggcagt caacccactt cttttttgga tttcttaatt ttcgtctgat aaagccgctt
     1141 tacccgttca atttctgcca gcgttgcgga ccagtgtagt tcggcggcat gacgatatct
     1201 ggcagccatt ttttccacca tctccggatt gctgtccgcg ctgcggtgct gacgcccctt
     1261 ctccccaaaa acttcaacaa ccagccactg gcttccggtt ctgccgaaca atgccttgca
     1321 caaacgtctc acagtcagcg gggttaaatc tggatcggga tcgtaggcaa tggtagcaaa
     1381 aataaagcgc agcatttctg atgtcttgta acgctttacg ctggtagcca ggcgtgaggc
     1441 acgttgcggt gcttcccagc ggtcgttact gtaattagcc acttcagcca gataatcctt
     1501 ttcaagtttt tcagccgtat agcacctgcc gcctaaaaaa agccgcatgg cctccctggt
     1561 tttgtctctc acatatcatc cctcattaat gctaatgtac gcatacatta gcattaacat
     1621 gctgacagca acaagcggca gatttacgtc cggataacgt aaatgattgc ggtatacagg
     1681 ccaggcggtc agatttccag ccgtgcctgt tcctgcagca gttccagatg atatgcagca
     1741 atagcgttta ccacccctgc cgtcatccgt gcgtcggcga tcgctgagtg tgccagccct
     1801 tcccaggtca gtccggcctg gctggcagca ctggccagtg aaatcgtgcc atagcggttg
     1861 gtggccccat aatatcctgc tgccagctcc atcgcacaat ataccgtcat ttcttccagc
     1921 cagtaagccg gatcgctatg tgcggcagca gtctgtttca gaatgcggat gtcgaaccgg
     1981 gcattaaaga taattactgg tcggcccccg atagcatgac gcagttgccg cgccacatct
     2041 gtccatgatg gtgcgccgca gagagcctgc tcactgatac catgcacagc ggcggcctgc
     2101 gccccgatgg cgaccgtggg cttcagtctg gtttcaaata ccacctgacc ggccgcatca
     2161 gtaaggccga tttccagggc ttccgcggta ttatccagtc ctgtcgtttc agtatccaga
     2221 aagagcggtg ccagactcag ccagtcatat gcctgccgtg ccatccggcc actggtgctg
     2281 ttaagtctgg ataaaacgga aagtcgctgg ccagccagaa gttgtttact cgtcagtggg
     2341 cgtttctcac gcatcggaac acaatctgca attcggtata cagcgaacct gccaccataa
     2401 gtgtttttat accacttaac cggctcagca ccgggggcag gcttcattct gaattcatcg
     2461 cgcagacgtc ctttcgagaa acatctgtct tcagcaatcg cctgcgcacg tctggtggcc
     2521 agctcatcat tattcatggt tcttgcatct ccgtcagacg tttttatatc cggacagcct
     2581 ctctatggcc acttctgtgc agtatgcaat acacgcagga tatacacttt tccccactgg
     2641 ctgtcgacct cataaaccag aacaaaatgc gggtgaataa ccagttcccg ggtaccttcc
     2701 acacgaccgt tgcggccttt atacggctgc ccggccaggc tgctggctga ataaccaatc
     2761 cgttcgtcca gttcaatggc ggccatcagg ttctgctccg caatgtactc acgaatgcgc
     2821 ttcctgtcct ggctggccag catagtccag aatatctcca tcatttaccg gcgaccttcc
     2881 tgctcatttt atcccgccat gctgcagcag aggattcgac catgtcatgg ggaagcacat
     2941 cgccgtgctc gagttgctga cgtcctgccg caacctggtc gcggaaccag gtattatgct
     3001 gccgttcaac ggtctggcgc ataaaatccc ggattaactg agagccatta cggtccatgc
     3061 tttttgcagc ggccataaaa gcatccttca gttcagcg
//
LOCUS       DAZUCC010000353         3073 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZUCC010000353 DAZUCC010000000
VERSION     DAZUCC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.355"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..945
                     /locus_tag="ACHY09_004982"
     CDS             52..945
                     /locus_tag="ACHY09_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3887808.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(976..1965)
                     /locus_tag="ACHY09_004983"
     CDS             complement(976..1965)
                     /locus_tag="ACHY09_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS3887809.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQSIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(1992..2843)
                     /locus_tag="ACHY09_004984"
     CDS             complement(1992..2843)
                     /locus_tag="ACHY09_004984"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS3887810.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
BASE COUNT          737 a          745 c          789 g          802 t
ORIGIN      
        1 gcccccttca tgagtgtgtg tgaagaggat gcttctacaa ggaaatcgcc aatgctcaaa
       61 gaaaacttca acgaactgca aatctttctt gtggtggcaa gagagcgaag ttttaccaaa
      121 gcagcgggca aactcggcgt ctctcagtct gcacttagcc atgcaataaa ggctctggag
      181 gaaaggctca atatccgcct cctgacacgg actacccgca gtgtggctcc cacagaagcg
      241 ggggagagaa tcattgcctg ccttgaaccg cgtattgacg agcttgaaca ggaactggaa
      301 tcacttattc aattgaacgg cacgccctcc gggaatattc ggttatctgc cggggaacat
      361 gccgcgcgaa gtctggtctg gccgaagcta aaaccttttc tcagagagta tcccgaaatc
      421 aatgtcgaac tggtggtgga taacggtttt gtcgatattg ttgaaggccg ttttgatgcc
      481 gggatacgac tgggtgaaaa tgttgataaa gatatggttg cagtcaggat tggaccagat
      541 atgcggatgg ctgtggtggg agctccggcg tattttgctg caaaccctgc gcctgaaacg
      601 cctcacgagc tacaaaatca tcgctgcatc aacatgcgtt tgccaactgc cggtgggctt
      661 taccactggg agttcgagcg agaagggaaa ccgcttcgcg ttaaggttga tgggcaactg
      721 acgtgtagtc cattgccaga aagaattgat gccgcgttat cagggtttgg gatcgcttgt
      781 gtccctgaag acatggttca ggagtatata gagtcaggca agcttattca ggtgttacag
      841 gagtggtgtc caacttttcc tgggtattat ctttactacc ccagccgcaa gcagcatccg
      901 ccagcttttg cgctgttgat cgatgcgctt cgctacaccg aataacgggt cctttatcct
      961 ggacccgttt gggtattagc ggcctacgcg tgcctgatgt tcaggcgagt aacgttcacc
     1021 gacgatttta attgtttcca gcgcctcgct tatctgctgc gtatctttct gcgaaagaac
     1081 aatgtcagca gcggccaggt tttcttccag ccggtgcagt ttagtggtgc cagggatggg
     1141 aacaatccac ggcttttgtg ctaatagcca tgccagtgcg atttgtgcag acgttacccc
     1201 tttctccgca gccagttcac ctaacaaggt gaccagtttt tcattggctt caatggattg
     1261 tgctgcgaaa cgcggtacgg tgctgcggta atcatctttg ccgaaagtgg ttcccggctt
     1321 aatcgcccct gtcaggaaac ctttacctag tgggctgaag gggacaaaac caattcccag
     1381 ttcctccagc aacggcagaa tttcctgttc aggctcacgc caccacatgg aatattcgct
     1441 ttgcagggcg gtgacaggtt gtaccgcatg tgcacgacga atggtttgtg ctcctgcttc
     1501 agacaggccg aaatgtttga ccttaccttc tgcgatgagg tctttcaccg ttcctgcaac
     1561 atcttcaata ggaacatctg gatcaacacg gtgttgatac agcaggtcaa taacatctgt
     1621 tttcagacga cgtaatgatc cttcaacggc ttcacggata tgttccggac ggctgtttaa
     1681 aatctgctgc ttattatcat cgccgaaggt aaaaccgaat ttagtggcaa ttaccacgcg
     1741 gtcacgaaac ggttttaagg cttcgccgac cacttcttca ttcagatacg gaccatacac
     1801 ttcggcggta tcgaagaagg tgacgccacg ttcaaccgca gcacgaataa gttcgatagc
     1861 ctgacgggta tcggtcgccg ggccgtagcc gtggcttaag cccatgcaac cgagcccaag
     1921 ggcggagact tcgagtccgg atttacccag ataacgtttt tgcattgaat gaatacctct
     1981 tttatcgcgg cttaaacatc aagtttgcgg ccagtcagcc attccaccat tgcgggatcg
     2041 cggtgagaga agaacgcgct ggttgcggta tccagggcgg taatttgcag catatcttca
     2101 gggctgagtt caaaatcgag aatgttgatg ttctcttcca tgcgttcttt tcgcactgat
     2161 tttgccagtg aaacaatgcc tcgttggtag atccagcgca gaacaacctg gcccacgctt
     2221 ttgccatact tctcgccaat tgccgtgaga acaggatgct ggaacagacc atttttccct
     2281 tccgcaaacg gtgcccaggc ttctggctga atgccacgac tttgcatcca gggaacggcc
     2341 tgtagctgct gattgaaagg gttaacttca acctggttta ccgccggaac gacgttatta
     2401 aaagcgataa ggtctgccag tcggtcagga tggaagttac tgacgccaat agcgcgaatt
     2461 ttaccggcct gttgcagctc ttccatggca cgccaggctc cgtggacatc gccatatggc
     2521 tggtgaatca agtacaggtc aacgtaatca agttgcagtc gattcagaga gcgttcgaac
     2581 tgggctttgg ccccttcgta actggtatcc tgcaaccaga gtttcgtggt gacaaagagt
     2641 tcgttacggg cgataccgct ttgtttcagt gcgttcccga cctggatttc attttgataa
     2701 gaggcggcgg tgtcaatcag tcgataaccg ctgttaatag cgtcaatgac cgctctttcg
     2761 cattcggcgg catcggtcat ctgaaataca ccaaagccca acaggggcat ttcaataccg
     2821 ttgttcagtt ttactttttg catggtgtta tccttccgtt atcgttcatg agaagcataa
     2881 cgtaaaggga agagctcgat tagacggcag gatttgtcag gggttatgaa caaaattcat
     2941 aaatctgttt gagggttgag attagtcgtg caagttcgat atccccggaa tttacagtcc
     3001 gatgaataaa aatgaatgtc cataaaaaat atattggttt atcccagcgt atatacttac
     3061 ttcgacgtac tcc
//
LOCUS       DAZUCC010000354         3062 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZUCC010000354 DAZUCC010000000
VERSION     DAZUCC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.356"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2024
                     /locus_tag="ACHY09_004985"
     CDS             <1..2024
                     /locus_tag="ACHY09_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS3887811.1"
                     /translation="YAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTS
                     AERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARG
                     TAQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKDADGSIQTLYVPYSSV
                     PVLQRAGYTRYALAMGEYRSGNNLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEEYQAF
                     NLGIGKDLGLFGAFSFDITQANTTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRY
                     STQGFYNLSDSAYSRMSGYTVKPPTGDSNEQTLFIDYFNLFYSKRGQEQISISQQLGH
                     YGTTFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFT
                     LNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGN
                     TQGGNTSSGTSGYSSLNYRGAYGNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQP
                     LGDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRVALNANSLADNVEL
                     DETVVTVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAEN
                     GQVYLTGLPQSGKLQVSWGKDKNSNCIVEYKLPAVSPETLLNQQTAICR"
     gene            2038..2568
                     /locus_tag="ACHY09_004986"
     CDS             2038..2568
                     /locus_tag="ACHY09_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3887812.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            2581..>3062
                     /locus_tag="ACHY09_004987"
     CDS             2581..>3062
                     /locus_tag="ACHY09_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3887813.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     "
BASE COUNT          881 a          664 c          711 g          806 t
ORIGIN      
        1 actatgcata tttgaattta cagagtggca tcaacattgg tagttggcga ctgcgcgata
       61 actcaacgtg gagttataac agtgggagta gcaattcatc tgacagcaat aaatggcagc
      121 atatcaatac gtcggctgaa cgtgacatta ttcccttacg ctcacgctta acggtaggtg
      181 atagttatac cgatggcgat atttttgata gtgtaaactt tcgtggcctc aaaataaatt
      241 caacagaagc gatgttgccc gatagccaac atggtttcgc tccggtgatt catggtattg
      301 cccgcggcac cgcacaagtg agtgtaaaac agaatggata cgatgtttat cagactactg
      361 tcccacccgg cccttttact attgatgata tcaactctgc ggccaatggt ggtgatctgc
      421 aagtaaccat aaaagatgca gacggcagta ttcagacatt atatgttcct tattcgtctg
      481 ttccggttct ccaacgtgct ggatatacgc gttatgcgct tgccatgggg gaatatcgta
      541 gtggaaataa cctgcaaagc tcccccaaat tcatacaagg tagcttgatg catggactgg
      601 aaggaaactg gacaccttat ggcggaatgc aaattgcaga agaatatcag gccttcaacc
      661 ttggtattgg taaagattta ggactttttg gtgccttttc tttcgatatc acgcaggcca
      721 atacgacgct tgcagatgac acccgtcaca gcggacaatc ggttaaatcc gtctacagca
      781 aatccttcta ccagacggga accaatatcc aggtcgcagg atatcgctat tctacgcaag
      841 gtttttataa cttatccgac agtgcctaca gtcgaatgag tggttacacc gtcaagcctc
      901 ctaccggaga cagcaatgag cagacactat ttattgatta ttttaatctg ttttacagta
      961 agcgtggtca ggaacaaata agcatctctc agcagcttgg acattacggt acgacatttt
     1021 tcagtgccag tcgccaaagt tactggaaca cgtcacgcag cgaccagcaa atatcatttg
     1081 gattaaatgt gccgtttggt gatattacga cttcgctgaa ttacagctat tccaataata
     1141 tatggcaaaa cgatcgggat catttacttg cttttacgct taatgttccc ttcagtcatt
     1201 ggatgcgtac agacagtcag tcggcatttc gtaattcaaa cgccagttac agtatgtcaa
     1261 acgatttgaa aggcggcatg accaacctat caggggttta tggcactctg ctgccggata
     1321 ataacctgaa ttatagcgtt caggtcggta acacccaagg gggtaataca tcgtctggca
     1381 ccagtggtta cagttctctt aattatcgtg gagcttacgg taatacgaat gtcggttaca
     1441 gtcggagtgg tgacagcagc cagatttatt acggaatgag tggtggcatt attgctcatg
     1501 ctgatggcat cacctttgga cagccgctgg gcgacacaat ggttctggtt aaggctcctg
     1561 gcgctgataa tgtcaaaata gagaaccaga ccggaattca taccgactgg cgtggctatg
     1621 ccatattacc atttgcgaca gaatatagag aaaatcgtgt cgctcttaac gcgaattccc
     1681 ttgcagataa tgttgaactg gatgaaaccg ttgtcactgt catcccaact cacggtgcta
     1741 ttgccagagc aacatttaat gcacaaatcg gcgggaaagt attaatgacg ttgaagtacg
     1801 gtaataagag cgttccattc ggtgcaattg tcacacatgg ggagaataaa aatggcagca
     1861 ttgtcgcgga aaacggtcag gtttatctga ctggacttcc acagtcaggg aaattacagg
     1921 tttcatgggg caaagataaa aactcaaact gtattgtcga gtacaagctt cctgcagtct
     1981 ctcctgaaac cttgctgaac cagcagacag caatctgtcg ctaaggaaca atagattatg
     2041 aaatacaata acgttatttt cctcggttta tgtctggggt taaccaccta ttctgcttta
     2101 tctgcagata gcgttattaa aattagcggg cgcgttctcg attatggctg cacagtctca
     2161 tcggattcgc ttaattttac cgtagatctc caaaaaaaca gtgccagaca atttccaacg
     2221 accggtagca caagtccagc cgtccctttt cagattacgt taagtgaatg cagtaaaggg
     2281 acaacggggg ttcgggttgc atttaacggt attgaggatg cagaaaataa tactctgttg
     2341 aaactggatg aaggaagcaa cacggcctcc ggtttgggta tagaaatact ggacggaaat
     2401 atgcgtccgg tgaaattgaa tgaccttcat gccgggatgc agtggatccc actggtacca
     2461 gaacagaaca atattttgcc ttactccgct cgtctgaagt caactcagaa gtccgtcaat
     2521 ccgggactgg tgagggcttc ggcaaccttt acccttgaat ttcaataatc agagccagat
     2581 atgaaaagac ttcacaagag gttcctgtta gcgacgtttt gcgcgttatt cacagcaact
     2641 ctccaggccg ccgatgtcac tatcactgtt aatggtcggg tagtcgctaa accctgcact
     2701 attcaaacca aagaagctaa cgttaatctc ggggatcttt atacgcgcaa tctgcaacaa
     2761 cctggttctg catcaggctg gcacaatatt actttgtcat taaccgattg tccggttgaa
     2821 acaagtgcag tgacggcaat cgtgacaggt tcaactgaca atacgggtta ttacaaaaat
     2881 gaaggtactg ccgaaaatat tcagatagag ctaagggatg accaggatgc gacgttaaaa
     2941 aatggcgata gcaaaacggt tattgttgat gagatcactc gtaatgcaca gtttccactt
     3001 aaggcaagag ctatcacggt gaatggaaac gcaagccagg gaacgatcga ggcgctaatc
     3061 aa
//
LOCUS       DAZUCC010000355         3059 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZUCC010000355 DAZUCC010000000
VERSION     DAZUCC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.357"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..352)
                     /locus_tag="ACHY09_004988"
     CDS             complement(<1..352)
                     /locus_tag="ACHY09_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS3887814.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVL"
     gene            complement(385..1302)
                     /locus_tag="ACHY09_004989"
     CDS             complement(385..1302)
                     /locus_tag="ACHY09_004989"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFS3887815.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKKMKII"
     gene            1420..1972
                     /locus_tag="ACHY09_004990"
                     /pseudo
     CDS             1420..1972
                     /locus_tag="ACHY09_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2206..2892)
                     /locus_tag="ACHY09_004991"
     CDS             complement(2206..2892)
                     /locus_tag="ACHY09_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFS3887816.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSIASMMAQMQMGDVKALFENKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVDGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
BASE COUNT          871 a          768 c          581 g          839 t
ORIGIN      
        1 gcaaaactgt atattctgcc catgcggctg cctgggcatc atttatcaca cttccattta
       61 aaccagttat atcttccatt atatttttca gaggatactc tttcagccct ccaagattgt
      121 ctggattcaa agctgtcaat actccattat taataatccc tgttgatgct actgcaaatc
      181 gctccgctgt tcccttcatt tcagaaacca gtagacgtaa agcccctgtc atttcgtcag
      241 gacatctttc atgaggtgtt gatatctgtt ttttcagcaa aaatgaaccg tcatcagata
      301 tcaacgccgc tgaaattttc gttcctccta tatcaacagc cagtgttatc attcatttta
      361 cctatataac aatcttgcaa ttattcagat tattttcatt tttttatttc tgtcatgttc
      421 ttcgagcagt cgctctgcaa gttttgataa tacatccaga ttcttttcct ttaccggaga
      481 aaaaggactt ctgcatacag gagtatttat aatccccata tagtaaagaa gagtttttat
      541 acctgccagg acaccactct gaataagagt atcaataacc tggttacagg ccacctgcat
      601 ttccttcgcc ttaatcacat cattattttt gactgcttca aatatttcaa gataacgcca
      661 ccccattata ttatatgtac taccgattcc cccatctgca cctgcaatta accctgatgc
      721 aaatatttca tcgtaaccat tatataacac aagctctgga tgattccttt tgatttgttc
      781 catctggaat aaatcaccgg atgtctgttt taatgcacaa accccgggta ttgtgacaag
      841 ctcatttatc tgatctaaag aaaaacgcac tccgctaaga gccggtatat tataaactac
      901 catcggaagt ccatctgcag aatcaataat cttcctgtaa tggatacaat gctcttcaaa
      961 tgaaaaagga taataaaatg gagtaacagc agatatagca tgatatccgt atttttttgc
     1021 agcttttgca agtacctcgc tttctgccgt actgattccg cccacatgcg ctataagtgt
     1081 tagtcttcca tctgattcat cggctactgt ttcaagaatt ttttctcgct ctgcaacatt
     1141 ctgaagaaat gcttctccag tagagcctcc aacatacaat cccttaatat tttgttcgat
     1201 attaaaacgg atcagttttc gaagactctc catatcgatt tgctgggatt ggtcataagg
     1261 agtgggtaat gctgatatga cccctttaaa ttcacattgc atttttaatc accttcactt
     1321 aactgcataa tgggatacct agtacaccat caagtacaaa gattatccaa cagaaaagaa
     1381 gcacaatgtg aagattatca aaaaaaagcc atcagaaagc tgtcatgccg ctcagttaag
     1441 gatcggttga tcgatccagt gtccagttac tattctctgc gtaaccctct gcgactatct
     1501 tgccctggaa ctgattaccc acagccctga agagtccgac actgtcacgc tacacaaacg
     1561 gcgtcttcct ctggaaatga tggtctggtg aattatcagt atggcccatg agcgtaagga
     1621 accttcatca gattgtgaac cggctggaca tcatgctgcc aggcagtagc gccgtgctcc
     1681 atgcccgcca gcggccagga agcggagcca tacgctgcgt gttcactcaa acagcgcagc
     1741 tctagcataa caccacgcca tatccgctct ggtgcgatct gaatctgctg accattgatg
     1801 gcgtgttctg acgaacacag gacacgcaag aacacgataa agcttcactc gccaaataca
     1861 tagtggaaac cagacacttt atcctcaggt aaaaatggtc tgtctgatag aactgaccag
     1921 ccatctactg acggcagcgg ccttcgatac aatgaatctg gcactaaata agactggcaa
     1981 tatagccgat aatgcgttga atcagtggct atatcgtata cataaaacag acatcattat
     2041 tctgtcttgt tgtccaacac ataaaaacta ataaaaacat accattccct gtcgtcagag
     2101 aatttattga taaacaggct ttattgaaca aatcaaacat tcagggaact ggcagttgaa
     2161 caaaacgcca gtccgaagac tggcgcagtg ataaaacatt ctgcatcatg ccacattctg
     2221 ctcctgaaga tgacgcagac gatggtccca gtttcccagg taatgcccgg gagtcagcag
     2281 tacgcggcgt cgctcattac agagcgagtt accaatcacc acttctccgt ccacaccaca
     2341 caacgacagt tgtatatacg ccatgccggc tgccagcggg tcaatatccg ttgccgacac
     2401 ccacatcctg tggtatgccg gataccccga acggttcagg atgtcagcca ttgccagaat
     2461 catactgccg gcaccacagg caggttcact gagcgtgata aagggtttgt tctcaaacaa
     2521 cgccttcaca tctcccatct gcatctgtgc catcatgctg gcaatactcc agggcgtgaa
     2581 aaactgactc cggtatttat cccccagttc cagttgcatg aacacactcc ccagaaaatc
     2641 acagaatccc tgctccagtc cctcaatgac atgagcaagc aaatgcgcaa tgtggataac
     2701 atcctgcttt tcataaccac tgacgattcg cagatacttc tgttcccggg cctcactgaa
     2761 ctgaagccgg ttttccaggg caatcacact acagctgaca aagtcttcaa acaccttatg
     2821 acgtttgtga taacaggctg tccggttaaa caacgaaatg aactccttct gatggtcagt
     2881 catggtggcc atgttttctc cgtaaaaaca gggaacacat ggcccggaca ggggaacagg
     2941 tgttcccctt cgggttgaaa tgttgataaa acgggcatca cggacatgat acccggaggt
     3001 taattactgg gctgagacca gaggaaccgg taaaggtgcg gctttcgtct gttcttcag
//
LOCUS       DAZUCC010000356         2993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZUCC010000356 DAZUCC010000000
VERSION     DAZUCC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.358"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..79)
                     /locus_tag="ACHY09_004992"
     CDS             complement(<1..79)
                     /locus_tag="ACHY09_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="induced by CusR in the presence of copper; YedW
                     induces the expression of the upstream gene yedV (encoding
                     a sensor kinase) as well as yedW; yedVW is one of four
                     copper regulons found in E. coli; part of the copper
                     homeostasis mechanism; confers resistance to copper and
                     several drugs when induced; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFS3887817.1"
                     /translation="MSFEDSEKASRVTLQQHYNFVMNQAV"
     gene            101..514
                     /gene="hiuH"
                     /locus_tag="ACHY09_004993"
     CDS             101..514
                     /gene="hiuH"
                     /locus_tag="ACHY09_004993"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFS3887818.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            623..1627
                     /gene="msrP"
                     /locus_tag="ACHY09_004994"
     CDS             623..1627
                     /gene="msrP"
                     /locus_tag="ACHY09_004994"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFS3887819.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTHERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            1628..2263
                     /gene="msrQ"
                     /locus_tag="ACHY09_004995"
     CDS             1628..2263
                     /gene="msrQ"
                     /locus_tag="ACHY09_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFS3887820.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSL"
     gene            2520..>2993
                     /locus_tag="ACHY09_004996"
     CDS             2520..>2993
                     /locus_tag="ACHY09_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFS3887821.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKA"
BASE COUNT          814 a          689 c          682 g          808 t
ORIGIN      
        1 aaacagcctg gttcattaca aaattgtaat gctgctgtaa ggttaccctg gacgcttttt
       61 cgctatcctc aaaactcatt cacatgacaa ggatataaac atgttaaagc gttatttagt
      121 actctccgta gtaacggctg cattttcatt accttctttg gtctatgctg cacaacaaaa
      181 cattcttagc gtgcacattt tgaaccagca aacaggaaaa cccgctgccg acgtgacagt
      241 cactcttgaa aagaaagcgg ataacggctg gttacaactt aataccgcca aaacagataa
      301 ggatggacga attaaggcac tgtggcccga gcaaactgcg actacgggcg attaccgtgt
      361 cgtatttaaa accggggact atttcaagaa acaaaatctt gaaagtttct tccctgagat
      421 ccccgttgaa tttcatatta ataaagtgaa cgagcattat catgtgcctt tattacttag
      481 ccaatatggg tattcaacct atcgtggcag ttaatgcata aatatataaa agcagatctc
      541 tgctactgtt agggaggtga ctccctgaca gattaacagt aaacggctct tgctggctaa
      601 cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt ttaaaagaat cagatgttac
      661 ggccgagtcg gtattcttta tgaagcgtcg gcaggtgtta aaagcactgg gcatcagcgc
      721 ggctgcacta tctttgcctc acgctgcgca tgccgatctg cttagctggt ttaaagggaa
      781 cgatcgtcca cccgcccccg ccggaaaacc gctggagttc agcaagcctg ccgcctggca
      841 aaataacctg ccactgacgc cagcagataa agtttccggt tataacaact tctatgaatt
      901 cgggctggat aaagccgatc ccgccgctaa tgctggtagc ctgaaaaccg atccatggac
      961 actgaaaatc agcggcgaag tagcaaaacc attaaccctc gatcacgatg atttaacccg
     1021 tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc gtggaagcat ggtcgatggt
     1081 ggtgccgtgg attggttttc cgctgcacaa attgctggcg cttgccgaac ccaccagcaa
     1141 tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa cagatgccag gccagcagga
     1201 ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa ggattgcgtc tcgacgaagc
     1261 aatgcatccg ctcacactga tgaccgtagg tgtttatggc aaggcgttac cgccacaaaa
     1321 tggcgcgccg gtgcgactga ttgtgccgtg gaaatatggc tttaaaggga ttaaatcgat
     1381 cgtcagtatt aagctgaccc acgagcgtcc gccaaccacc tggaatctgg cagcgcctga
     1441 cgaatacggt ttttacgcca acgttaatcc gcatgttgat cacccgcgct ggtcacaggc
     1501 taccgaacga tttattggtt caggcggcat cctcgatgta cagcgccagc caacgctact
     1561 gtttaatggt tacgccgagc aggtggcatc gctgtatcgt ggcctggatt tgcgggagaa
     1621 tttctaaatg cgtctgacag caaaacaggt gacatggctg aaagtttgcc tgcatcttgc
     1681 cggattgttg ccgtttctct ggctggtctg ggcgattaat cacggtggat tgggtgccga
     1741 tccggtgaaa gatattcagc attttaccgg tcgcactgca ctgaaatttt tgcttgcggc
     1801 cttgttaatc acacctctgg cacgctacgc aaaacagccg ttattgatac gcactcgccg
     1861 cctgttaggc ttatggtgct ttgcctgggc gacactgcat ttaaccagtt acgcattact
     1921 ggagctgggc gtgaataatc tggcgttatt aggtaaggag ttaattaccc ggccttattt
     1981 aacgttagga attatcagct gggttatttt gcttgcttta gcgtttactt caacccaggc
     2041 gatgcagcga aaactcggca agcactggca acagttgcat aatttcgtct atcttgtcgc
     2101 gatcctggcc ccaatacatt atctgtggtc agtgaagatt atttcaccgc agccactcat
     2161 ctacgctggg ctggcggtac tgcttttagc cttacggtat aagaagttgc tttccctttt
     2221 caaccggtta cgcaagcagg ttcacaacaa attatctcta taacttgata ttgccgacaa
     2281 agtacgcgtt atccctgccg gatgcggcgc gagcgcctta tccggcatac acagatcgca
     2341 ttaggttatg tttcgtttgt catcaacctc aaatcatcga cataatttat tttatttcaa
     2401 taagatagat aagcacacct gagagaacta ttctcttgtc tcctaagagt tgttgcattt
     2461 tgttatatgt tacaatataa cattacacat catatacatt aactctggag gaaactgttt
     2521 tggcgattcg tcttcataaa ctggctgttg ctttaggtgt ctttattgtt agcgctcctg
     2581 ccttttcgca tggtcatcac tcacacggca aacccttaac agaggtcgaa caaaaagctg
     2641 ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac gctaagtgac tgggatggag
     2701 tctggcaatc cgtttatcct ttattgcaaa gtggcaaact tgaccccgtc tttcagaaga
     2761 aagcggatgc agataaaact aaaacatttg ctgaaattaa agattattat cacaaaggtt
     2821 atgcaacaga tatcgagatg attggcattg aggacggcat tgttgaattc catagaaata
     2881 atgaaacaac atcctgtaaa tatgattacg atggatacaa aatactcacc tataaatcag
     2941 gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc tgaaagcaaa gcc
//
LOCUS       DAZUCC010000357         2976 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZUCC010000357 DAZUCC010000000
VERSION     DAZUCC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.359"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(8..1522)
                     /locus_tag="ACHY09_004997"
     CDS             complement(8..1522)
                     /locus_tag="ACHY09_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFS3887822.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLVDREPGPVMVFNGEKNVPADSLKSQRLIYPDSNNHTGK"
     gene            complement(1674..2414)
                     /locus_tag="ACHY09_004998"
     CDS             complement(1674..2414)
                     /locus_tag="ACHY09_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFS3887823.1"
                     /translation="MNKVIAVSALAMAGMFSTQALADESKTGFYVTGKAGASVMSLAD
                     QRFLSGDGEETSKYKGGDGHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTG
                     ISTWDYGYGSSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSSVS
                     YKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            2638..2823
                     /locus_tag="ACHY09_004999"
                     /pseudo
     CDS             2638..2823
                     /locus_tag="ACHY09_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          849 a          720 c          673 g          734 t
ORIGIN      
        1 ataccattca cttacctgta tgattattac tgtcaggata aataagtctc tgtgacttaa
       61 ggctgtctgc aggaacattt ttctcgccat taaacaccat aaccggcccc ggctctctgt
      121 ccacgagcgg gttgaatggc ggtatatttt cagtacgaat gccggtcagc cactgaaaaa
      181 tacctgcgaa atgacgggca ctgattttcc tgctgacgga ctgatgagac gtgatgtcac
      241 tggcggtaat aatcagggga acgctgtagc ctccctgcac atgaccatca tgatgaacag
      301 gattggcact atcgctgacc gacagcccat ggtcagaaaa gtaaagcatg gcaaaatgac
      361 gggaatgccg gcgaaggata ccatcaagct gaccgagaaa gttatccagt ttactgatgc
      421 tggcgaggta acaggcaatc tttcggggat actgctccag gtaatgattc ggccaggagt
      481 taagtcggtc acacggattc ggatgagagc caatcatgtg caggaaaatc accttcggag
      541 aggatttatc cgccagcgca cgttctgttt cctgtaacaa caacatgtca tccgttttac
      601 gggaagcgaa gctgcctttc ttgaggaaaa cggtatgctc cgcatcagaa gcaataacag
      661 agatgcgtgt gtcatgctcc cccagttttc cctgattgga tatccaccat gtgctgtatc
      721 ctgcttttgc tgccagcgcc accacgttgt tgccggagtc agggttctgc tcatagtcat
      781 aaatcagtgt ccggctcagg gaaggtacgg tactggctgc tgccgatgta tagccgtcaa
      841 taaataaacc gggagccgta ttcagccacg gtgtggttgg tacgggataa ccatataccg
      901 acatataatc cctgcgcaca ctctcaccag tgacaatcac aatcgtgtca tacaacggtg
      961 ctcccggcag gattttccag ctgtcagccc tgtgctgact cagttgttca taacgctgca
     1021 tttcacgcaa tgtgtcagtt gtccccacaa cggttccttt aaccatccgc aacggccagc
     1081 tgtttacaga gcataatacg aacagcagca gtgccagcca gttacggtga ccgcggtggt
     1141 gtgtccgcca gaaaataacc atgaacacca gaatcgcagc actgaccaga aaatgataaa
     1201 caggaatcat cccggtaaac tccgcagcct catctgccgt ggtctgcaac aacgcgacaa
     1261 taaaactgtt gttgatttta ccgtacgtca tacccgcagg cgcatacagt gcacaacaga
     1321 acagaaataa cagagctgta atggatgtaa gggtatttct gtgtgcaagg agcagaagaa
     1381 ggaacagcag caacacattc ccggtggcat tcttctcagt gtatccgcat gcaattgtgg
     1441 ttatgacaga aataacaaaa aagaataaaa acaataaaat ccgtggtgtg ttgtccggac
     1501 aaaacagttt tctgatattc atcggagtat atcgacaaca ttattgtgaa gagaataaaa
     1561 atcagaagtc tcctgtgaaa cagataacaa acaaccctgc agtataatta ctgcagggag
     1621 tcttcgttta attacagaag atgggtaatt attatctttc aggagcagtt ttgtcagaaa
     1681 ttataagtca caccaagcat gatgtcatga cttttaacat caacttctga tttatattta
     1741 tcgccccact cgtccttgta actcacactg ctgtcacctg catcaagata gcgatagctg
     1801 aggtccagag cgatatccgg ggttacgtca tagcgaacac ccgcgccaag gctccatgcg
     1861 aagttgtcag cagagcctga acgtgacaac gattcgcgac cactgcttcc gtacccataa
     1921 tcccaggtac tgataccggt tgttttctgg tgaattctgg cgtagccaat ccctgcagat
     1981 acccatggtg tgaatgcact gtcattccgg aagtcatagt acgcattcag catcagtgtg
     2041 ttgactgaca cctcattctt caggtcatca cgccagtaac cgcctgacca gctgtcttta
     2101 tctacgttat acttcgaatc agcttttcca cgagcgtaaa actccagttc cgtacgaacc
     2161 ggaatactga actgcgggta aaaatcataa ccggccgcga taccgccact gaataccgta
     2221 tcatggccat caccgccttt atattttgat gtttcctctc catcacccga caggaaacgc
     2281 tggtctgcaa gtgacataac agaagcacct gctttaccgg tcacataaaa gcctgttttg
     2341 ctctcatcag ccagagcctg ggtcgaaaac atgcctgcca tggcaagcgc tgaaaccgca
     2401 ataaccttat tcatttcaat catctccgct atcactttcc ccgaacgata aatggggttg
     2461 ctcattctaa aaccatgcaa aagacagggt aaaaggaatg ttttcgatcg aatcgatcga
     2521 taaaatcgat cgtgcccgat aaagttttct tatcgtcccc ataaaaatgg taagggctgg
     2581 tttaagaggt aatcgcagcg gcatggaagt attaagacca aaacagcacg agataaagcg
     2641 gctgaacgtc agatgagcca catggaacgt aattcattaa gggcggctta tatccataaa
     2701 acagaacacc tggaatagcg tcggttgatg ctacagtggt ggggggattt tctggataca
     2761 aactcgaaag aaaataccag cccctttgag tatgcgaaga ttaacaatcc attaaaatat
     2821 taagcactcc gggcaatctg cccggagtgc tttagctggc ataacgggct actgatctcc
     2881 tctggttaca gataacgact tcagccctct gaaagagaaa agcggaagct caggaaggtt
     2941 gtgatttgct tttaaccttt ttaatttaga agtatt
//
LOCUS       DAZUCC010000358         2941 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZUCC010000358 DAZUCC010000000
VERSION     DAZUCC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.360"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2246
                     /locus_tag="ACHY09_005000"
     CDS             <1..2246
                     /locus_tag="ACHY09_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HFS3887824.1"
                     /translation="TKSAGSYTSAPVKAPPKEWLANNKKNQSVMQNEDSEDDNLATFT
                     LSGGRNVEYRITATQRQNFSKTTGALARWIFPGEMITVSGIEVTGGHFYLGQRMKPAG
                     HNSGGYYDGNNEASLVDDTLKIYPTPYIYEDSSLGYWPSFSSLSPEARGAYLSWLASD
                     RCDTSCPIGYVFIYLYGLERRALVDSRDNNISDDEFYSLFKEICRLRSVFNENRSFRN
                     YSSQLLEAMSILRPNENLAAALNDDSEFSNSMQFRLILAKTVDTGAPVPADLALTWVT
                     NHAEYSLRTPARRCAKEFAALFKRRYTLKYGEGMVVKPNKARLRLDYTPASPSLRGIR
                     LPVPDLPDPGALKGPVQKLMAIADICTGELDAYSRYLGRKGTSANDTAAILLLPSEIV
                     NESAEKILSTFKHWADDAIRSKGGIVSVADYWSHMNATCPAKINKKEADLMQAFAQKM
                     GYCLAPDPYHHHVKADVDGVLVLFPAGERGRFSPYPEFITAVLTLRLGSVVALIDNSL
                     DQAEQKVLENAINNNASFSDDEKRSLHAYLTWQLHTPANMTGMKSRIELLGAAEKSAV
                     GKVIVSVACSDGRITPAEIKQLEKIYTSLGLDPSSVSSNIHQHSATETALVSSVPANQ
                     STAGFTLDANVLARHESATDDVRKLLNSIFTEEEPEQPESAPASLTGEGGLDSAHSQL
                     YRRLLEKEQWSRKEATELCSNWNLMLGGALEVINDWSYAVVDAPVLEDADDHIWIDLE
                     IAKELEG"
     gene            2250..2651
                     /locus_tag="ACHY09_005001"
                     /pseudo
     CDS             2250..2651
                     /locus_tag="ACHY09_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
     gene            complement(2622..2830)
                     /locus_tag="ACHY09_005002"
                     /pseudo
     CDS             complement(2622..2830)
                     /locus_tag="ACHY09_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          809 a          674 c          694 g          764 t
ORIGIN      
        1 aaactaagtc cgcgggctct tatacttccg ctcctgtgaa agcaccacct aaagaatggc
       61 ttgctaacaa caaaaaaaat cagtctgtaa tgcaaaacga ggacagcgaa gatgacaatc
      121 tggctacgtt tacactaagt ggcgggcgga atgtcgaata caggataaca gccactcaac
      181 gccagaattt ctcaaaaact actggtgctt tggctcgttg gatttttccg ggagagatga
      241 tcacagtcag tgggatagag gttaccggcg gtcacttcta tcttgggcag cgtatgaaac
      301 ctgccgggca taactcaggc ggttattatg atggcaataa cgaggcatcg ctagttgacg
      361 acacgcttaa aatatacccc acaccttaca tttacgaaga tagttcatta gggtattggc
      421 ccagtttttc ctcactttca cctgaagctc gtggtgcgta cctgagttgg cttgccagcg
      481 acagatgcga cacttcctgc ccaattggtt acgttttcat ttatctgtat ggtctggaaa
      541 gaagggcgct tgttgacagc agggacaaca atatatccga cgatgagttc tattctcttt
      601 tcaaagagat ctgccgtctc agatcggttt tcaatgaaaa ccgatctttc cggaattact
      661 catcccagtt acttgaagcg atgtccattc ttcgtccgaa cgaaaatctt gcagcagctc
      721 tcaacgatga ttcggagttc agcaatagca tgcagttcag gttaatcctg gcaaagactg
      781 ttgatacagg agccccggtt ccagcggatc tggctctgac gtgggtgaca aaccatgctg
      841 aatactcatt acgaaccccc gcccgccgct gtgcgaaaga atttgctgca ctatttaaac
      901 ggcgttacac ccttaaatac ggtgaaggga tggtagtgaa gccgaataaa gcgcgtctgc
      961 gtttagatta cacgcctgca agtcccagtt tgcgaggtat tcggcttcct gttcccgact
     1021 tgcctgatcc aggtgcattg aaaggcccag ttcagaagct tatggccatt gccgatatat
     1081 gcacaggtga actggatgct tacagtcgtt accttggtag aaaaggtact tcagcaaatg
     1141 ataccgctgc aattttgttg ctgccttcag aaatcgttaa cgaaagcgca gaaaaaatat
     1201 taagtacatt caaacactgg gctgatgacg caatccgcag taagggtggg atagtttcag
     1261 ttgctgacta ctggtcacat atgaatgcca cctgtccggc gaagatcaat aaaaaagagg
     1321 ccgacttaat gcaagccttt gcacagaaaa tgggatactg tctggctcct gacccctatc
     1381 accaccacgt aaaggcagat gtcgatggtg tacttgtctt attccctgct ggtgaaagag
     1441 gacgtttctc accttatcct gaatttatca cagctgtatt gacgctccga ttaggttcgg
     1501 tagtcgcctt gattgacaat tctctcgatc aagccgaaca gaaagtgctt gagaatgcca
     1561 tcaacaacaa tgccagcttc agcgatgatg aaaagcgctc tctgcacgca tatttaacgt
     1621 ggcagcttca tactccggcg aatatgacgg gaatgaaaag ccggattgaa ttactcggtg
     1681 cagcggaaaa atcagctgtg ggtaaggtta tcgttagtgt cgcttgctct gacggcagaa
     1741 ttacgcctgc cgaaatcaaa cagcttgaga agatttatac aagcctggga ctggatccct
     1801 catccgtatc aagcaatatc catcagcatt ccgcaactga aactgctctc gtttcatctg
     1861 taccagctaa tcagtcgacg gcagggttta cgctggatgc caacgtactt gcccgccacg
     1921 aatctgccac tgatgatgtt cgtaaattac tgaactcaat tttcactgaa gaagaaccag
     1981 aacaaccaga aagcgctcca gcctcattaa ccggggaagg gggacttgac tccgctcata
     2041 gccaacttta tcgcaggttg ctggagaaag aacaatggtc cagaaaagag gcgacagagt
     2101 tgtgtagtaa ctggaacctg atgctcggcg gtgcgcttga ggtgattaac gattggtcct
     2161 atgcggtggt tgatgcacca gtgctggaag atgctgatga tcacatttgg attgacctgg
     2221 aaatagccaa agaattagag ggttagtgaa tgtctgtaac acgtataaga gtgaaagagc
     2281 gcgacgctat cattcagtca cttaagtcgg gagtaacgcc aagaataggt attcagcata
     2341 tccaggttgg ccgcgtgaat gagataaccg ccctccatca ggatattgaa cggattgctg
     2401 atggcggggc aagcttcaga cttatcattg gtgagtacgg ttccgggaaa acattctttt
     2461 taagtgttgt acggtctatt gcgttagaga agaagctggt cacggtcagt gcagaccttt
     2521 ctcccgacag acgtatccac tcatcgggag gtcaggctcg taatctctac tcagagctta
     2581 tgaaaaatat gtccaccaga aacaaaccag atggcaatgc attacttagc gttgtggaaa
     2641 ggtttgttac agtcaaggca gtcaccaaac tcgataataa agcggctcat ttccatacgt
     2701 ctgtccttaa ggggcattgt tcatttctgt gaccaagccg caccaccttt tttactgatt
     2761 cgtctgtcgg gaacactttg cgtttcttga tgacataacg gatcacacta ttcaacgact
     2821 ctatggcatt ttgcacatac ttccatagca actgtcatcg gtagctactg aaccagcaag
     2881 ttaagtaagt tactacgtga aaattttatt tttccaacgg tttttccata atcattaata
     2941 t
//
LOCUS       DAZUCC010000359         2926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZUCC010000359 DAZUCC010000000
VERSION     DAZUCC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.361"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(72..389)
                     /gene="bolA"
                     /locus_tag="ACHY09_005003"
     CDS             complement(72..389)
                     /gene="bolA"
                     /locus_tag="ACHY09_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFS3887825.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            694..1272
                     /locus_tag="ACHY09_005004"
     CDS             694..1272
                     /locus_tag="ACHY09_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS3887826.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            1316..2791
                     /gene="ampG"
                     /locus_tag="ACHY09_005005"
     CDS             1316..2791
                     /gene="ampG"
                     /locus_tag="ACHY09_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFS3887827.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT          636 a          762 c          724 g          804 t
ORIGIN      
        1 ggcccgcagg ccgtttttca taaaatcttt agcaacatac tggaaaagcg ccgacagttg
       61 caaatgcgtt tttacgcgat gcttcctgct ccacgacagg gaggagaggc aaagacggtg
      121 tcctgcaacc cttcccactc cttaatagtg taagtatgca gagccagcgc atgaacggta
      181 gtagagagtt cctccgctaa agtactgtaa atcattcgat gacgattcag aaaacgttca
      241 cccgtaaaac gatcgctgac cagcacaact ttaaaatggc tttcagagcc ggctgggaca
      301 ttgtgacgat agctttcatc cactacttcg aggaatacgg gttggaacgc cgcccttaat
      361 ttttcttcta tccgctcacg tatcatcatg aaatatctcc tccgacaacg ctgagatgtc
      421 atccatccct ttaaatacta gccgctttta ccgtttccat tgcagcttaa caacaaatat
      481 ttaggtttcg tcctggtttt tctgtcaagc gtatgaagtt tactgattta aagattgaaa
      541 cacaggcctc gcatcagcgt ttcttttacc aggggcgatt atttcgtcaa catgatgaat
      601 ttacatgcct tacccacttc ccctcgccgt tggcaatgtt atgatggcgg gaatttttac
      661 caaacacgct taagagtctc tgagagcccg aacatgttca aaaaaatcct cttcccgtta
      721 gttgctctgt ttatgcttgc aggatgcgca aaaccgccaa caactattga agtttccccg
      781 acgattacac tgccacagca ggatccaagc ctgatgggcg tcaccgtaag cattaatggt
      841 gccgatcagc gtaccgatca ggcgctggca aaagtcaccc gcgataatca aatcgttacc
      901 ctgaccgcct cccgcgatct gcgtttcctg ctgcaagaag tgctggaaaa acaaatgacc
      961 gcccgtggat acatggttgg tccgaatggc ccggttaatc tgcaaatcat cgttagccaa
     1021 ctgtatgctg acgtgtccca gggcaacgtg cgctacaaca tcgcgaccaa agcggatatc
     1081 gccatcatcg ctaccgcgca gaatggcaac aaaatgacca aaaactatcg tgccagctac
     1141 aacgttgaag gtgcgttcca ggcctccaac aaaaatatcg ccgatgcggt taacagtgtg
     1201 ctgaccgata ccatcgctga tatgtctcag gacaccagca tccacgaatt catcaagcag
     1261 aacgcgcgtt aatttctgcc ctctggcccg gtgcaagccg ggcctgtaga cgcccatgtc
     1321 cagtcaatat ttacgtattt ttcaacagcc gcgttcagcc atattgctga tcctgggttt
     1381 tgcttccggg ctaccgctcg ccctgacgtc cggcacctta caggcctgga tgacggtcga
     1441 gaatatcgat ctcaaaacca ttggtttctt ctctctggta ggccaggctt acgtttttaa
     1501 attcctctgg tcaccactga tggaccgcta cacgccccca tttttcgggc ggcggcgcgg
     1561 ttggctgctc gccacgcaaa ttctgttatt agtcgccatt gcggcgatgg gttttctcga
     1621 accaggcacc cagctccgct ggatggcggc actggcggtg gtgatcgctt tttgctctgc
     1681 ctcccaggat attgtcttcg atgcgtggaa aaccgatgtg ctcccggcag aagaacgtgg
     1741 tgcgggcgcg gcaatcagcg tgctgggtta ccgtttaggg atgctggttt ccggcggctt
     1801 agccctgtgg ctggcagata aatggctggg ctggcagggc atgtactggt tgatggcggc
     1861 gctgttgatc ccctgtatta tcgcgacgtt gcttgcacca gaacccaccg acaccattcc
     1921 tgtgcccaaa acgctggaac aagcggttgt tgcacctctg cgagatttct ttggtcgcaa
     1981 taatgcctgg cttattttgc ttcttatcgt gctgtataag ctgggcgacg cattcgctat
     2041 gagcctgaca accacgtttt tgattcgcgg cgtcgggttt gatgcgggtg aagtaggcgt
     2101 ggttaacaaa acgcttggct tactggcgac cattgttggc gcattgtacg gtgggatttt
     2161 gatgcagcgc ctgtcgctgt tccgtgcact gctgattttc ggcattttac aaggtgcatc
     2221 taacgccggt tactggctgc tgtcgattac tgataagcat ctctacagca tgggcgcagc
     2281 cgtctttttc gaaaacctct gtggcgggat gggtacatca gcctttgtcg cgctgttaat
     2341 gacgctgtgt aataagtcat tttccgctac tcaatttgcc ctgctctcag cgctttctgc
     2401 tgtagggcga gtttatgtcg gccccgtggc gggttggttt gttgaagcac acggctggtc
     2461 gacattctat ctattctccg tcgccgctgc cgtaccaggg cttatcttgc tgctggtttg
     2521 tcgccagacg cttgaatata cacgagtaaa tgataacttt atctcccgta ccgaatatcc
     2581 ggcaggttat gcctttgcca tgtggacact ggcggcgggc gtcagcctgt tggccgtgtg
     2641 gttactgctg ttgacgatgg acgcgctgga tttgacgcac ttctctttcc tgcctgctct
     2701 gctggaagtc ggggttttag tcgccctttc tggcgtcgtg cttggtggtt tactggatta
     2761 tctggcgcta cgaaaaacgc atctgacgta atctgtaaat attatttagc cgtagtaatc
     2821 actcgccgta attattacgg ctaaataaac atcagcatcg cttattaata tttgttcttt
     2881 tgtgcggctt agcgtctgga aattattgac gccatttatg aattct
//
LOCUS       DAZUCC010000360         2891 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZUCC010000360 DAZUCC010000000
VERSION     DAZUCC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.362"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            161..2440
                     /gene="ydeP"
                     /locus_tag="ACHY09_005006"
     CDS             161..2440
                     /gene="ydeP"
                     /locus_tag="ACHY09_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFS3887828.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTIGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2688..2885
                     /gene="safA"
                     /locus_tag="ACHY09_005007"
     CDS             2688..2885
                     /gene="safA"
                     /locus_tag="ACHY09_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFS3887829.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          789 a          667 c          689 g          746 t
ORIGIN      
        1 atttatcgta tttaatcttt tgtttaacga taatagatta aaatagaaag agagtagtcc
       61 cctactctta tatatccatg ttgacgataa tccctttgta tatactgtgc atcatcgcta
      121 ttacaaatcc taataattca tttccacaca ggataagcag atgaagaaaa aaattgaatc
      181 ctaccaggga gctgccggtg gttggggtgc tgttaaatcc gtagcaaatg cagtacgtaa
      241 gcagatggat atacgccagg atgttattgc catgtttgat atgaataagc cagagggctt
      301 tgactgtccg ggatgtgcat ggccagatcc taagcacagt gcgtcattcg acatttgtga
      361 aaacggcgca aaagcaatcg cctgggaagt cacggataag caggtaaatg cctctttctt
      421 tgctgagaat acggttcaat cattacttac ctggggagat cacgagcttg aggctgcggg
      481 acgtctcact cagcctttga aatatgatga cgtcagcgac tgttacaagc cattaagctg
      541 gcaacaagct ttcgacgaaa ttggcgaacg ccttcaaagc tatagtgatc ccaatcaggt
      601 tgaattctat acttcgggcc gcacttccaa tgaagctgcc tttctttatc agctttttgc
      661 ccgtgaatac gggagcaata acttccccga ctgctccaac atgtgccatg aaccgaccag
      721 tgtgggtttg gcagcgagta tcggtgtagg taaagggacc gtgttgctgg aagactttga
      781 gaaatgcgat ttagtcattt gcattgggca taaccctggt acaaaccacc ctcgcatgtt
      841 gacttcgttg cgcgctttag tgaaacgggg agcgaaaatg atcgccatca atcctctaca
      901 ggaacgtggc ctggagcgat ttaccgcacc gcaaaacccg tttgaaatgc tgacgaactc
      961 tgagactcag ttggccagtg cctactacaa cgtgcgcatt ggtggtgata tggcgttgct
     1021 caaggggatg atgcgcctgt taattgagcg cgatgatgct gcaagcgccg caggtcggcc
     1081 ctcattgctg gatgacgaat ttattcaaac gcataccatc ggctttgacg agctacgccg
     1141 tgacgtgctc aattccgagt ggaaagatat cgaacgtatt tctggactaa gtcagacaca
     1201 aatcgccgaa cttgctgatg catatgccgc tgccgaacga accattatct gttacggaat
     1261 ggggatcact cagcacgaac atggtaccca gaacgtacag caactggtca atctgctgtt
     1321 gatgaaaggt aacattggta agcctggtgc gggtatctgc ccactacgag gtcactctaa
     1381 tgtacagggc gaccgaaccg tcggtatcac cgagaaaccg tctgcagagt ttctggctcg
     1441 cctgggtgag cgctatggtt tcaccccacc tcatgcacct ggacatgctg caattgccag
     1501 catgcaagca atatgtacgg ggcaggctcg agcattgatc tgcatggggg gcaactttgc
     1561 actggcaatg ccagatcggg aagcaagcgc tgtaccgtta acacaattag atctgacggt
     1621 acacgtagcc actaagctta accgctctca tctgctgacc gcacggcata gctatattct
     1681 gccggttctg ggacgcagcg agattgacat gcaaaaaagc ggtgcgcagg cggtaaccgt
     1741 tgaggattca atgtcgatga ttcatgcctc gcgtggcgtg ttaaaacccg ccggtgtaat
     1801 gctgaaatca gagtgtgcag tggtcgcggg aatcgcgcag gcagcactac cccagagcgt
     1861 ggtagcctgg gagtatctgg tggaagatta tgatcgcatt cgcaatgaca ttgaagctgt
     1921 gctgccagag ttcgccgact ataaccagcg catccgtcat cccggtggtt ttcacctgat
     1981 aaatgcagct gctgaaaggc gctggatgac gccgtcaggt aaggctaatt tcattaccag
     2041 taaagggctg ttagaagatc cctcttcagc gtttaacagt aaactggtca tggcgacagt
     2101 acgcagccac gatcagtaca acacgacgat ttatggtatg gatgatcgct atcgaggtgt
     2161 attcggtcaa cgagatgtgg tctttatgag tgctaaacaa gctaaaattt gccgtgtaaa
     2221 aaacggcgaa agagttaatc ttattgcgct cacgccagac ggtaaacgta gctcacgccg
     2281 catggataga ttaaaagtgg tcatttaccc tatggctgac cgctcactgg tgacctattt
     2341 tccagaatcg aatcacatgc taacacttga taaccacgat ccattaagtg gcattcctgg
     2401 ctataaaagt attccggttg aattagaacc atcaaattaa ttcgcctcct catttcttct
     2461 gctgtcatcc gcacagcaga agaattcctc attgaccatt gtcgcgcaat ttactcacat
     2521 gtgctaaatt aaactgctcg ttataagata tatacctccg cctacagctg taagaaactc
     2581 cgctcagtac tgaagcgcca gtcctatttc ccctattctc cagcctgtta tattaagcat
     2641 actgattaac gatttttaac gttatccgct aaataaacat atttgaaatg catgcgacca
     2701 cagtgaaaaa caaaatcacg caaagagaca actataaaga aatcatgtct gtaattgtgg
     2761 ttgtcttatt actgacactt acgttgatag ccattttttc ggcaattgat cagctgggta
     2821 tttcagaaat gggtcgcatt gcaagggatc ttacacattt cattatcaat agtttgcaag
     2881 actgaaaata a
//
LOCUS       DAZUCC010000361         2890 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZUCC010000361 DAZUCC010000000
VERSION     DAZUCC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.363"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(141..217)
                     /locus_tag="ACHY09_005008"
     tRNA            complement(141..217)
                     /locus_tag="ACHY09_005008"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(181..183),aa:Asp,seq:gtc)
     gene            complement(350..1081)
                     /gene="dnaQ"
                     /locus_tag="ACHY09_005009"
     CDS             complement(350..1081)
                     /gene="dnaQ"
                     /locus_tag="ACHY09_005009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFS3887830.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQDETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1146..1613
                     /gene="rnhA"
                     /locus_tag="ACHY09_005010"
     CDS             1146..1613
                     /gene="rnhA"
                     /locus_tag="ACHY09_005010"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFS3887831.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1610..2332)
                     /locus_tag="ACHY09_005011"
     CDS             complement(1610..2332)
                     /locus_tag="ACHY09_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS3887832.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGLPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2366..>2890
                     /gene="gloB"
                     /locus_tag="ACHY09_005012"
     CDS             2366..>2890
                     /gene="gloB"
                     /locus_tag="ACHY09_005012"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFS3887833.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFA"
BASE COUNT          770 a          710 c          691 g          719 t
ORIGIN      
        1 ataaatgaga caacaagcga gagttacgca cttacgccag agtaaaatca ttgaacagag
       61 aaatggcggg attcaaatac aaaaaaaccg caaaaataaa aatttttgcg gttctctgaa
      121 ctcattttca tgagtgaatt tggcggaacg gacgggactc gaacccgcga ccccctgcgt
      181 gacaggcagg tattctaacc gactgaacta ccgctccgcg ttttgttccg ttgggaacga
      241 ggcgaatatt acggattgcc tcacccttcg tcaacggttt ttctcatctt ttgaatcgtt
      301 tgctgcaaaa atcgcccaag tcgctatttt tagcgccttt tacaggtatt tatgcccgcc
      361 agaggcagct tccccccttc ttctgcacca gatcgagacg ggcttcatgt gctgcaagct
      421 cttcatctgt cgcaaaaaca acgcgtaact tacttgcctg acgcacaatg cgctgaattg
      481 ttgtttcatc ttgttgctgt tgtgtctctc cttccatcgc aaaagccatc gacgtttgcc
      541 caccagtcat cgccagataa acttccgcaa ggatctgggc atcgagtaat gccccgtgca
      601 gcgttcgttt actgttatct atttcgtagc gggcacataa cgcatcgagg ctgttgcgct
      661 taccgggaaa cattttcctc gccaccgcaa ggctatcggt gaccttacag aaggtattgg
      721 tcttcggaat atcgcgctta agcaacgaaa actcgtagtc cataaagccg atatcgaacg
      781 ctgcgttatg gatcaccagc tccgcgccgc ggatatagtc catgaactca tcggctacct
      841 ctgcaaacgt gggcttatcg agcaaaaatt catcggcaat accatgtacg ccaaaggctt
      901 ccggatccac cagccgatcg ggtttgagat aaacatggaa gttattgccc gtcaggcgac
      961 ggttcaccac ttcaacggca ccaatctcaa tgatcttgtg gccttcatag tgcgcaccaa
     1021 tctggttcat accggtagtt tcggtatcga gaacgatctg gcgtgtaatt gcagtgctca
     1081 tagcggtcat ttatgtcaga cttgtcgttt tacagttcga ttcaattaca ggaagtctac
     1141 cagagatgct taaacaggta gaaattttca ccgatggttc gtgtctgggt aatcctggac
     1201 ctgggggtta cggcgctatt ttacgctatc gcggacgcga gaaaaccttt agcgcaggct
     1261 acacccgcac caccaacaac cgtatggagt tgatggccgc tattgtcgcg ctggaggcgt
     1321 taaaagaaca ttgcgaagtc attttgagta ccgacagcca gtatgtccgc cagggtatca
     1381 cccagtggat ccataactgg aaaaaacgtg gctggaaaac agcagacaaa aaaccagtaa
     1441 aaaatgtcga tctctggcaa cgtcttgatg ctgcgctggg gcagcatcaa atcaaatggg
     1501 aatgggttaa aggccatgcc ggacacccgg aaaacgaacg ttgcgatgaa ctggctcgtg
     1561 ccgcagcgat gaatcccaca ctggaagata cgggctatca agtcgaagtt taagcctgtg
     1621 gtttacgaca ttgccgggtg gctccaaccg cctggcgaat tcgtggcttg tttttactct
     1681 gtttcatcgg atttagcgtt aaaggaatgg tccgtttccg ggcaacaata agttgtaagc
     1741 aaccaagcgc aggaatatgc gcattcaata gttttcctcc gtgtttgttc cacgggagaa
     1801 cgtggaaacg gctggcgtgt agcacttcaa aattcaacaa agagagccag tccagctgcc
     1861 gcatcagagt aaacatccgg ctgttatagg gcgaggtttt gcgcaatacc ggcacaagtt
     1921 tgcgtaatcc cattaaactg atgggattga agccactaat caccagccag ccatcatcaa
     1981 tcaatacccg atcggcttca cgcaataaac gatgcggatc ggtgcaccac ggcaatgtat
     2041 gtgccagtag acaaacatca acggatttat cggcaaaagg aagatgaagt gggtccgcct
     2101 gcacctggac gggcagtcct tgcgcagaaa cattcacttg atgagaaacc gcgcacgctt
     2161 cgcaattgat ttctgcgctt aaattgccaa tcttaagcag atgaaaacca tacattttag
     2221 tgaaccacgg gttgagctgg cgctccagcg ccttgcgata aagctctccc cagggcaaat
     2281 cgccccagca atcaggagcc acgacagttt gagggactct tgccggtttc atcacaacct
     2341 tccgtttcac actgagaggt aatctatgaa tcttaacagt attcccgcct ttgatgacaa
     2401 ttacatctgg gttttgaatg atgaagcagg tcgctgcctg attgtcgatc ccggagacgc
     2461 agagccagta ttaaacgcca ttgccgccaa taactggcaa ccggaggcca tatttctcac
     2521 ccaccatcac cacgatcacg ttggcggcgt aaaagaactg gtgaaaaagt ttccgcaaat
     2581 tgtggtgtat ggtccacaag agacacaaga taagggaaca acacaggtag tcaaagatgg
     2641 cgaaactgcc ttcgttttgg ggcatgaatt tagtgtaatt gccacgccgg gtcacacttt
     2701 aggacatatc tgttacttca gtaaacctta tctattttgc ggcgacacgc tgttttctgg
     2761 tgggtgtggt cggttgtttg aagggacagc atcacaaatg tatcaatcac ttaaaaagtt
     2821 aagtgcgtta cctgacgata cattggtatg ttgtgctcat gaatatacct tatcaaatat
     2881 gaagtttgct
//
LOCUS       DAZUCC010000362         2850 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZUCC010000362 DAZUCC010000000
VERSION     DAZUCC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.364"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..840
                     /locus_tag="ACHY09_005013"
     CDS             <1..840
                     /locus_tag="ACHY09_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFS3887834.1"
                     /translation="LENAAERAQALKADIDSQIQMFEQRLKPLNEAVLFSAPEGIALT
                     SGEDVQLAAGQNLAVNAGGDISTGTMGNLTALAGERLGLFARSGQLSVKSGEGPVEVQ
                     AQNGSMRLFAEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTTVSYLRKV
                     KRTIAAGAASQQPEMPWLADPVKPSASALMQYLYHDDVPVVGAPFKATLADGTVREGN
                     LDGAGYLHLEDVPPGRVLVELGPDTRDYLRKNKTDNRLFVGTSLSEADIDSLIAKYSE
                     GVE"
     gene            837..2381
                     /locus_tag="ACHY09_005014"
     CDS             837..2381
                     /locus_tag="ACHY09_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887835.1"
                     /translation="MSGVNVAWFKETSLETGQWIWGTLQGAFNEKQTIGQIIVDAVIG
                     MIPLVGDVTAVRDLIAVSIGLAKEPKKRESVTEWVFLVILLFALIPVIGGVIKGVGRL
                     ALRVTTKVAENVETLGAVIKFLNRIGEGDAVKWLNKLNVVEYQAQLIEKMGDFCNTMI
                     TAINKILKARVGRMLPEKWQADLLLVRDGFAALQRLASRMIPQALKELDAKLRVLQAL
                     VYKGEIHTITTGMGRAEVRREAEAYLIERPLCEAPKQGTRYPSVHAEVIHEDRLRLKY
                     SREGYPDLLGYKGKSPAFGDEQVFTNIASFSGEITAKSASEMGGSTIFRAFGNPTELA
                     PNGSWAAGGFWGYQKVPGSAEEWRQLSAVLDEWNGNGFLVVLHLPEDLATRMPGSNAW
                     HGKIAEQFGQKRPDQYLQGGGEQVIINLDETLKTELNRIGEQVKASGQAVSIEINGVN
                     AEFYPTGWKEVEGVHGYSKPEGIEPPYVTYTRELHDDELRSKVTERTILNAARIEYQT
                     TTGSEQ"
     gene            2378..>2850
                     /locus_tag="ACHY09_005015"
     CDS             2378..>2850
                     /locus_tag="ACHY09_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887836.1"
                     /translation="MITIEKTKYPMSDEQVRRKLFWVLQRLSSYSLWQRKRDAWAYFT
                     QQYEQALKNWPEEITKGFDPEAIIWAYDALRLYDEGLSELAVGNRQVWQRLTGEFHQL
                     ARPVDLIESYFYLPCHERGVQQEPYPVEVEKLNKLKIAAKIHGDYLMYPPHNNVC"
BASE COUNT          753 a          573 c          893 g          631 t
ORIGIN      
        1 ctggaaaatg ccgcagaacg ggcacaggcg ctgaaggctg atattgacag tcagatacag
       61 atgtttgagc agcggctgaa accgctcaat gaagccgtgc ttttctcggc gccggaaggc
      121 attgccctga ccagcggtga agatgtgcag ctggctgctg gtcagaacct ggctgtcaac
      181 gccggaggtg atatcagtac cggaacaatg ggcaacctga ccgcgctggc cggagagagg
      241 ctggggcttt ttgccaggag cggacaactg agcgtcaaat ccggggaggg gccagtagag
      301 gtacaggcgc aaaacggcag catgcggttg tttgctgaga agaagctgac gctgagttcg
      361 gcaagtgata tctcctttgc cgggaaaaaa cgtatcacgc tgattggcgg ggggagttac
      421 ctgcggctgg aggcggggaa aatagagtac ggtacgacgg tgtcgtatct gcgcaaagtt
      481 aaacggacta tagcggcggg ggctgcgtca cagcagccgg aaatgccttg gcttgccgac
      541 ccggttaaac cctctgccag tgcgctgatg cagtatctgt atcacgacga tgtcccggta
      601 gtgggcgcgc ctttcaaagc gactcttgct gatggcactg tacgggaagg taaccttgac
      661 ggtgcgggat atctgcatct ggaagacgtc ccgccaggga gagtgctggt ggagctcggg
      721 ccggatacca gagattattt acgtaaaaat aagaccgata atcggttgtt tgttgggaca
      781 tctctcagcg aggctgacat tgactcactg atagcgaagt acagcgaagg tgtggaatga
      841 gtggagtaaa cgttgcctgg tttaaggaaa ccagcctgga aaccggacag tggatctggg
      901 ggacgcttca gggggcattt aatgaaaaac agaccattgg gcagatcatc gttgatgcgg
      961 tgatcggcat gatcccgctg gtcggcgatg taacggcagt gcgagatctg atagcggttt
     1021 cgataggact ggcaaaagaa ccgaagaaac gggaaagtgt gactgaatgg gtgtttctgg
     1081 tgatcctgct gtttgcgcta atcccggtaa ttggtggcgt gataaaaggt gtcggacgtc
     1141 tggccctgcg tgtgacaaca aaagtggcag agaatgtgga aacccttggc gctgtgatta
     1201 agtttctgaa ccgtatcggc gaaggtgatg cggtgaagtg gctgaataag ctgaatgtcg
     1261 ttgagtatca ggcacagttg attgaaaaga tgggggattt ttgcaacacg atgattaccg
     1321 ccatcaataa aatcctgaaa gcccgggtcg ggcggatgtt gccggagaaa tggcaggcgg
     1381 atttactctt ggttcgggat ggttttgcgg cactgcaacg gctggcgtcg agaatgatcc
     1441 cgcaggcgtt gaaagagctg gatgcgaaac tgcgggtgtt gcaggcgctg gtttataaag
     1501 gtgaaataca tacaatcacg acggggatgg gacgtgctga agtgcgccgt gaggctgaag
     1561 catatcttat tgagcgtccg ctgtgtgaag cgccgaagca ggggacgcgg tatccgagtg
     1621 ttcatgcgga ggtaattcac gaagacagat tgcggcttaa atattccagg gagggatatc
     1681 cagatttgtt gggatataaa ggaaaaagtc cggcatttgg tgatgagcaa gtatttacaa
     1741 atattgcctc ttttagcggc gagattaccg caaaaagtgc aagtgagatg ggcggtagta
     1801 cgatttttcg cgcttttggt aatccaacag aattagcacc caacggttct tgggcggctg
     1861 gcggattctg ggggtatcaa aaagtgccgg gcagtgctga agaatggcgt cagctttcag
     1921 ctgtactgga tgagtggaat ggtaacggtt ttctggtggt gctgcatttg ccagaagacc
     1981 tggcaactcg tatgccgggt agcaatgctt ggcacgggaa aattgctgaa cagttcggac
     2041 agaagagacc tgaccagtat ctgcaaggtg gtggcgaaca ggtaattatt aatctggatg
     2101 aaacgttgaa aacagagttg aaccgcatcg gggagcaggt aaaagccagc ggtcaggctg
     2161 tcagtattga aattaatggc gtaaatgccg agttttatcc gacgggctgg aaagaggtgg
     2221 aaggcgtgca tggttacagt aagccggaag ggatagaacc gccgtatgtg acgtataccc
     2281 gtgaactgca tgacgatgaa ctgcgcagca aagtgacaga gcgaacgata cttaatgcgg
     2341 cccgcattga gtaccagacc acgacaggaa gcgaacaatg attaccattg aaaaaacaaa
     2401 atacccgatg agcgatgagc aggtacgtcg caaattattc tgggtgttac agcggctgag
     2461 cagctatagc ctttggcagc gcaaacggga tgcctgggcc tattttaccc agcagtatga
     2521 acaggcgctg aaaaactggc cggaagagat aacgaagggg tttgatcccg aggcgataat
     2581 ctgggcatat gatgctttgc gtctttacga tgaagggcta tcagaattgg cagtgggaaa
     2641 tcggcaggta tggcagcgtt taacgggaga atttcatcaa cttgctcgcc cagtggattt
     2701 aattgaatct tatttttatc tcccctgcca tgaacgaggt gtacagcaag aaccataccc
     2761 ggtagaagta gaaaagctta ataagctgaa gattgcagca aaaatacatg gtgactatct
     2821 aatgtatcca ccacataaca atgtatgcaa
//
LOCUS       DAZUCC010000363         2816 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZUCC010000363 DAZUCC010000000
VERSION     DAZUCC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.365"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1186)
                     /locus_tag="ACHY09_005016"
     CDS             complement(<1..1186)
                     /locus_tag="ACHY09_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HFS3887837.1"
                     /translation="MAKVKVKTLIGSVVTVAFFLTVIVAFLFYAFPENVASATGFGPY
                     DGLRILTFCAILVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESITPVTNTIPGEE
                     DDDEPVFSEDAVSEHLRFRYGRRWQRKVRLLLVMGDPDVVRKAAPGLCHDLWQEGDGY
                     VLIYGGDAQSLPDEIFLSKLKRLRSGQPVDGIIQVMSISALLTDGERDALLRYRQKAD
                     HQLGWQAPVWLWLTDNATGARQDAKAAATGVIFGPEGTVNGADEAFSTLALHLQKAGM
                     AQILNEPADFGLLQLSLRLRQELKASLAVLLSGLMQGTAAWRLRGVMFSPELTVAGAV
                     PNTCPDTPAWKAIIDDCDAVSGRKLGFNWLKMLRLILLSLIVLWGAGTLLSLLVNRTQ
                     IYQ"
     gene            complement(1179..2387)
                     /locus_tag="ACHY09_005017"
     CDS             complement(1179..2387)
                     /locus_tag="ACHY09_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887838.1"
                     /translation="MPVWLDAIPEKAPKIARPNTRRWLLFLAFIMLGGMALTLWCWTS
                     ERTGFVFWFTALGLPFCTWGLIFGLRRFVYKAGQVGAESRNVEREALIDSEILRGQRC
                     AWILGTYIQSPAGNKADDLLEAMEVAAPAIDFSHPRGCDKPVRYAALPEYQTDLTKAL
                     KGAVNKLTTRVEGIVHPLPPALPCWLVLDCDNDLYPLIEEQLKADLSLKTGRIFRLMT
                     GKGLGAFDAWLDKRWDTPGILVVITLSLPASPREEDADAVSMVVLSNRNTHAWPDALC
                     LHRPERGTETTLTKTLTRAFLWAKTRPNELKGSWISGPTLTSGSSWNNACEENGVTFS
                     LSEDNFSIDPVLGYTGHAAPWLAITLADAAFEQRGAQVIAAQPTADKDDVWVAVITKE
                     EVRKESPGNG"
     gene            complement(2390..2647)
                     /locus_tag="ACHY09_005018"
     CDS             complement(2390..2647)
                     /locus_tag="ACHY09_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFS3887839.1"
                     /translation="MLHIIRKGDKTTHGGSVLTASETMKFGGIGVARKGDKVSCPGHG
                     PTIIIEGNPDYLDHGIPVAFHGHKCACGCTLISSFAAAKVA"
BASE COUNT          669 a          793 c          708 g          646 t
ORIGIN      
        1 cctgataaat ctgagtacgg ttaacgagca gagacagcag tgtaccggcc ccccacagga
       61 caatcaggga aagcagaatc aatcgcagca tcttcagcca gttaaagccc agcttcctgc
      121 cgctgacggc atcacagtca tcaataatag ctttccaggc aggtgtgtcg gggcatgtgt
      181 ttggtaccgc tccggcaacg gtcagttcag gactgaacat gacaccgcgt aaacgccagg
      241 cagcagtccc ctgcatcagt ccactgagca aaacagccag gctggctttc agctcctgcc
      301 gcagtctgag tgacaactga aggagaccga aatcagcagg ttcattcagt atctgcgcca
      361 ttcctgcttt ttgcaggtgt aacgccagtg tggaaaacgc ttcgtccgca ccgttcacgg
      421 taccttccgg tccgaagatg acccctgttg cggccgcttt cgcgtcctgc cgggcaccgg
      481 tagcgttgtc agtcagccac agccagactg gtgcctgcca gcccaactga tggtcggctt
      541 tctgacggta acgcaggagg gcatcccgtt cgccatccgt caggagggct gaaatactca
      601 tcacctgaat gatgccatca accggttgac cggatcgcag acgcttcagt tttgaaagga
      661 agatctcgtc cgggagtgac tgcgcgtctc cgccataaat cagcacgtag ccgtcgcctt
      721 cctgccagag gtcatggcaa aggcccgggg ctgctttccg gaccacatcc ggatccccca
      781 tcaccagcag gagtcgcacc ttacgctgcc agcgacgccc atagcgaaag cgcaaatgtt
      841 ctgaaacagc atcttcgcta aaaaccggct catcgtcgtc ttcttcaccg ggaatggtgt
      901 tcgtcaccgg agtgatactc tcagcctttt tctgccccca ctggaaatac agcccggatt
      961 taccggcgaa gtcgaaagcc ttctccgtca gccagccgag taaaaacagc gtggcaacca
     1021 gtatggcgca gaatgtgagt atgcgcagcc cgtcatacgg accgaagccg gtggccgaag
     1081 cgacgttttc cggaaaggca taaaaaagaa atgccacgat gaccgtcaga aaaaaggcga
     1141 cagtcacaac agagccgatg agtgttttaa cttttacctt agccattccc gggtgattcc
     1201 ttgcgaactt cttctttagt gatgacggca acccagacgt cgtctttgtc agctgtgggc
     1261 tgggcggcga taacctgcgc acctcgttgt tcaaaggcag catcagcaag cgtgatggca
     1321 agccatggag cagcatggcc ggtatagccc agcacgggat cgatactgaa attgtcttct
     1381 gaaaggctga atgtcactcc gttctcttcg caggcattat tccagctgct gcccgacgtc
     1441 aatgtgggac cggagatcca gcttccttta agctcattgg gtcgggtctt cgcccagaga
     1501 aacgcccggg taagtgtctt tgtgagcgtt gtttctgttc ccctttcggg tcgatgaaga
     1561 caaagcgcat ccggccaggc atgggtgttg cgattgctta ataccaccat tgagacagca
     1621 tcagcatctt cttcccgggg agaggcaggt aacgacagcg tgatgacaac cagaatgccc
     1681 ggggtgtccc atcgcttgtc cagccaggca tcaaacgccc ccagcccttt tccggtcatc
     1741 aggcgaaaaa tcctgcccgt cttgagtgag aggtcggctt taagctgctc ttcaatcagg
     1801 ggatacagat cgttatcaca atccagcacc agccagcatg gtagcgcggg cggaagcgga
     1861 tgaactatcc cctctacacg cgtggtcagt ttattcaccg cccctttcag agccttcgtt
     1921 aaatctgtct ggtattctgg taatgccgcg tacctgaccg gtttatcaca tcctcttggg
     1981 tgcgagaaat caatagcagg agccgcgact tccattgcct caagcaggtc atcggctttg
     2041 tttccggccg ggctctgaat ataagtaccg aggatccagg cacagcgctg accacgcaga
     2101 atttcactgt caataagcgc ttcccgctcg acatttcggg actcagcccc cacctgcccg
     2161 gctttataga caaaccgacg taaaccgaaa ataagccccc acgtgcaaaa tggaagcccc
     2221 agagcggtga accagaagac aaatcctgtt cgttctgacg tccagcacca taatgtgagc
     2281 gccatccccc ccagcatgat aaatgccagg aacaacagcc agcggcgagt attggggcgc
     2341 gctatctttg gggccttttc agggatggca tccagccaga caggcataat caggcaacct
     2401 ttgctgcggc aaatgaactg atcaatgtgc aaccacaggc gcatttatga ccgtgaaatg
     2461 ccaccgggat accatggtca agataatcag gattgccttc tatgatgatg gtaggaccat
     2521 gacccggaca tgacacttta tcgcctttac gggcgacgcc tatcccaccg aatttcatcg
     2581 tttcagaagc ggtcagaacg gaacctccgt gagtagtttt atcgccttta cgaataatat
     2641 gaagcattat taaattcctc tttcttatct cttaactaat tcatcactct ttaattccca
     2701 tgtttatgct gggctctgcc atcgatagtg attgtctgga tttttattat gaatgtttcc
     2761 gggtcactga ttattgatag atagtgttcc ccatcgcatg tatttaccat tgagtt
//
LOCUS       DAZUCC010000364         2803 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZUCC010000364 DAZUCC010000000
VERSION     DAZUCC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.366"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(40..159)
                     /locus_tag="ACHY09_005019"
     CDS             complement(40..159)
                     /locus_tag="ACHY09_005019"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFS3887840.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            415..723
                     /gene="asr"
                     /locus_tag="ACHY09_005020"
     CDS             415..723
                     /gene="asr"
                     /locus_tag="ACHY09_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFS3887841.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            816..899
                     /locus_tag="ACHY09_005021"
     CDS             816..899
                     /locus_tag="ACHY09_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887842.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            999..1820
                     /gene="ydgD"
                     /locus_tag="ACHY09_005022"
     CDS             999..1820
                     /gene="ydgD"
                     /locus_tag="ACHY09_005022"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFS3887843.1"
                     /translation="MRRTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1859..2188)
                     /gene="mdtI"
                     /locus_tag="ACHY09_005023"
     CDS             complement(1859..2188)
                     /gene="mdtI"
                     /locus_tag="ACHY09_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFS3887844.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(2175..2540)
                     /gene="mdtJ"
                     /locus_tag="ACHY09_005024"
     CDS             complement(2175..2540)
                     /gene="mdtJ"
                     /locus_tag="ACHY09_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFS3887845.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
BASE COUNT          796 a          696 c          639 g          672 t
ORIGIN      
        1 ccggactgag gtaaataccc gtccggactt attgcctgct caaaccaacg ttaatagcca
       61 tcctaaaata gacgaagcgc cagccaattc ccgcagcgca tctagtgtca tcaggattat
      121 aagtacccaa ataaacggat tcattttgct gtgtgtcatt tattactgat gcgcagttat
      181 tctaccgctt tgtaagtagt aaaatagtta acacgatcaa gactactatt attggtagat
      241 gaattttcct taagtcacaa tacgttatta tcaacgctgt aatttattca gcgtttgtac
      301 atatcgttac acgctgaaac caaccactca cggaagtctg ccattctcag ggatatagtt
      361 atttcaacgg ccccgcagtg gggttaaatg aaaaaacaaa ttgagggtat gacaatgaaa
      421 aaagtattag ctctggttgt tgccgctgct atgggtctgt cttctgctgc ctttgctgca
      481 gagactgcga ccacacctgc tccgactgcg acgaccacca aagcagcgcc tgcgaaaacc
      541 acacatcata aaaaacagca taaagcagca cctgcccaga aagcgcaggc ggctaaaaag
      601 catcataaaa atgccaaagc tgaacagaaa gctcctgaac aaaaagcgca ggcagcgaag
      661 aaacacgcca agaaacacag ccatcagcaa ccggcaaaac ctgctgcaca acccgcagcg
      721 taagttttca acagtaatgc tggcgcgccc cctcgcgcct gaaaattacg gtgctaaacg
      781 ggtaacgttt agcaccgcct ttagcggagg gtagtatggt gggccgttat cgctttgagt
      841 tcattcttat catccttatt ttatgcgcac tgattgccgc ccgtttttat ctttcctgat
      901 cgtagttatc tgattttact cccactttca tcccgtcccg tctatagtat ttatgagggt
      961 ttgcttttaa taatcataat tacccaccag agtgtgatat gcgtagaacc attgctgtag
     1021 tgttgggtgc aattagtttg acgtctgctt ttgtgtttgc agataaacca gacgttgcca
     1081 aatcggcaaa cgatgaggtc agcaccctgt tttttggtca tgatgatcgt gtgccagtga
     1141 atgacacgac ccaatcaccg tgggatgcgg ttggccaact ggaaacggcc agcggcaatt
     1201 tatgtacagc gacgctgatt gcacccaatc tggcattaac ggcaggacac tgtttattga
     1261 cgccgccaaa gggtaaagcc gataaagcgg tggcgctgcg ttttgtgtca aataaaggtc
     1321 tttggcgcta tgagatccac gacatagaag gccgcgttga cccgacactg ggaaagcggt
     1381 taaaagcaga tggggatggt tggattgttc ctcccgcagc tgcgccgtgg gacttcggtt
     1441 tgattgtgct acgtaatccc ccttctggca ttacaccatt gccgttattt gagggagata
     1501 aagccgcgct taccgccgca ttaaaagcgg caggtcgtaa agtgactcag gcgggatacc
     1561 ctgaagatca tctcgatacg ttgtacagtc atcaaaactg tgaagtgact ggctgggcgc
     1621 aaacgtcggt gatgtcgcat cagtgcgata ccttgccggg tgacagcggt tcgcctctga
     1681 tgttgcatac cgatgacggc tggcaattaa ttggggtgca aagttcggct cctgccgcga
     1741 aagatcgctg gcgcgccgat aaccgggcca tttctgttac cggttttcgc gacaagctgg
     1801 atcaactgtc gcaaaaataa tgttcaaacg ctgcccgaca gcgcgggcag catgttcatc
     1861 aggcaagttt caccatgatc attccagcca acagcagcac caggccaatc cagcctttac
     1921 gatttaaccg ttgaccaaac aagatccaac ctgcggctaa cgtggcggca ataccaaacc
     1981 cgccccacaa tgcataagcg acagacaagt cgatcccttt aacggcctga gaaagcgcac
     2041 taaaggcagc cagcaccgcc gccagagaga gcaagccaaa tattttgcga cgaaagccgt
     2101 cagaaaattt caaaaagacg ttagcaacga tttccagcac gattgccaat gccagccagg
     2161 cggcgtgaac ccattcaaac tgcgccatgg ttcacctcca gttcaggttt acgcgcttta
     2221 cgggtacctg atttgatcaa cacaatcccg gcgaccaggg tggttaaccc ggcaattttc
     2281 atcagagata aactttcgtc gaataacaaa acgctaaaca aggtaataaa taaaataccg
     2341 ataccttccc acagcgcata agctacgcct aaggcgatct ttttaacggc gaaagagaga
     2401 aatatatacg acagagaaat catcaccagc attaaaataa agccgccatt tccctcactg
     2461 acgctcgccc atttcatgga cagcgtaccg gtaatttctg tagcaatagc cagacctaat
     2521 aaaatccaat aaatatacat tgtccttctc ctgcaagaga attattttaa ttttcgctta
     2581 attcagcgaa accaaagtaa agtggtcaag cccgcgctta gcgcaagttt tggcagaaga
     2641 aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga gcaagatagt ggatgaggta
     2701 cgaagatggg taaatgtaga aaataacgtc ctgaacaaat tgtccataat attacaatta
     2761 tccgcagtgt tgcttctcgt catcgcggat gataattgtc ctc
//
LOCUS       DAZUCC010000365         2749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZUCC010000365 DAZUCC010000000
VERSION     DAZUCC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.367"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..667)
                     /locus_tag="ACHY09_005025"
     CDS             complement(<1..667)
                     /locus_tag="ACHY09_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887846.1"
                     /translation="MTGLSEQAKELHMMMGRRKPPKIQAVLNTRYWKNTEKTIKRTVG
                     MNIHTLSQWCGEQYEKMFISKVAEVYMLHHTAAILRRKGVYVDVFKIPSKIMNPPEAR
                     ANCTGCPSSQNVSPFFAYSEPNTINFSESIRINYSYTLVPAYIRKKVSSLMGDSELRY
                     VYSFPGSLYSYLLEKHPLSEVKKLMEDLPSTEDENFRRSETGSQYLKGILGVKTRVSE
                     LFIR"
     gene            781..1665
                     /locus_tag="ACHY09_005026"
     CDS             781..1665
                     /locus_tag="ACHY09_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFS3887847.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICTTHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRHQELSLATVTVRVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIRLLVTLWDHLF"
     gene            1784..2461
                     /locus_tag="ACHY09_005027"
     CDS             1784..2461
                     /locus_tag="ACHY09_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887848.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            2467..2705
                     /locus_tag="ACHY09_005028"
                     /pseudo
     CDS             2467..2705
                     /locus_tag="ACHY09_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
BASE COUNT          696 a          695 c          620 g          738 t
ORIGIN      
        1 gccttataaa aagttcagat actcttgttt ttacaccgag aatccccttc agatactggc
       61 tacctgtttc actcctcctg aagttttcat cttccgttga aggcaaatcc tccatcagtt
      121 ttttaacttc tgacaatgga tgtttctcaa gaagataaga atataaacta ccgggaaatg
      181 aatacacata acgaagttct gaatccccca tcagagatga gacttttttc cttatatagg
      241 ctggaactag agtatatgaa taatttattc ttatactttc agaaaaattt atggtgttag
      301 gctctgaata agcaaaaaaa ggagagacat tctgagaaga agggcaacca gtacaattgg
      361 ctctcgcttc aggcgggttc attatttttg agggaatctt aaaaacatcc acataaactc
      421 ctttacgcct caatattgct gccgtatggt gaagcatata gacttctgcc acttttgaaa
      481 taaacatctt ctcatattgt tctccacacc actgactgag cgtatgaata ttcataccta
      541 cagttctttt tatcgttttt tctgtgtttt tccagtatcg ggtgttaaga acagcctgta
      601 tcttcggcgg ttttctccgc cccatcatca tatgaagttc ttttgcctgc tcagataatc
      661 ctgtcattta ttacctccga atatattcca ttaaaaatgg aaataattat acgcccatat
      721 tacctgaatg ataaaccgga ttactgtgat ccggttcagt gaaatcaaca ggataccggt
      781 atgtcattca gccattcttc cctctctgca caagtgaaat catatctgac gtttcttcct
      841 gaagaaattc gccagaaaat ccttgaacat ctccacggtg ttattcatta cgagcccgtg
      901 attggcatta tgggtaaatc cggcaccggc aaaagcagtc tgtgtaatgc catttttcag
      961 tcccgtatct gcaccacgca tcccctgaac ggctgcaccc gccaggctca tcgcctcacc
     1021 ctgcagattg gtgaacgcag aatgacgctg gtcgatctgc ccggcattgg tgaaacaccg
     1081 cagcatgatc aggaataccg ggagctttac cgtcagttac tgccggaact ggatctgatt
     1141 atctggatcc tgcgggctga tgaacgtgcg tatgctgccg atattgccat gcatcagttt
     1201 ttactgaatg aaggtgcaga tccctcgcgt tttctgtttg tactcagtca tgccgatcgc
     1261 gtgtttcctg ctgaagaatg gaacgacaca gaaaaatgcc cgtcccgtca ccaggaactc
     1321 tcactggcaa ctgttacagt ccgggtggca accctgttcc cgtcatcatt tccggtactc
     1381 cccgttgctg cgcctgtcgg ctggaatctt ccggcgtttg tgtcactaat gatccacgcc
     1441 ctgccaccgc aggcaaccag tgcggtttat tcacatatcc ggggggaaaa ccgctctgaa
     1501 caggcccgga aacacgcaca acagactttt ggtgaaacca tcgggaaaag ttttgacgtc
     1561 gccgttgccc gtttcagttt tccggcctgg atgttacagc ttctgcgtaa agcccgggac
     1621 cgcattatcc gcctgctggt cacactgtgg gatcacctgt tctgagcgcc ccggagtccc
     1681 ccgtggcaac cgtatacctt tacacgacaa cggattacgg taaccactgc cgtataccgt
     1741 ctgtcattat caataacaca ctctttaatt tcagaggtca gtcatgaata ccagaattct
     1801 ttttcccctg ctgttcactg tggcatcctt ctccgcctcc gccggtaact gggctgtcaa
     1861 aaacggctgg tgccagacca tgacggaaga tggtcaggcg ctggtaatgc tgaaaaatgg
     1921 cacgattggt attaccggcc tgatgcaggg atgcccgaat ggcgtacaga cgctcctggg
     1981 cagccgtatc agtattaacg gtaacctgat ccccacatca caaatgtgta atcagcagac
     2041 gggattcagg gctgttgagg tggaaatcgg acaggcgccg gaaatggtca aaaaagccgt
     2101 tcactccata gcagagcgtg atgtgtccgt tttacaggca tttggtgtac gaatggaatt
     2161 cacccgcggt gatatgctga aggtctgtcc gaaatttgtc acatcacttg ccggtttttc
     2221 cccgaaacag acgaccacta ttaataaaga ctccgtcctg caggctgccc ggcaggcata
     2281 cgcccgggaa tatgacgagg aaacaacaga aaccgctgat tttggctctt acgaagtaaa
     2341 aggcaataag gttgagtttg aagtattcaa tcctgaagac cgtgcgtacg acaaagtgac
     2401 cgtcacggtt ggtgctgacg gtaatgccac cagtgccagc gttgaattta tcggaaaata
     2461 gccggtatgt cggactgtca ccctgtttta ttgcccgaag gccctttctc acgcaaacag
     2521 gcaatggctg ccacaacagc ttaccttacc gcaatgtgct tattgaagat gaccatggaa
     2581 cgcatttcct gctggttatc cgcaatgccg aagggcagct acgctggcgg tgctggaatt
     2641 ttgagtctga cgccggaaaa cagcttaatg tgtggctcgc cagtgaaggg cttctcagac
     2701 aataaaagtc ttcatttcat ccatcaggcc gcgtcttctc cgggagacg
//
LOCUS       DAZUCC010000366         2746 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZUCC010000366 DAZUCC010000000
VERSION     DAZUCC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.368"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(98..>2746)
                     /gene="chiA"
                     /locus_tag="ACHY09_005029"
     CDS             complement(98..>2746)
                     /gene="chiA"
                     /locus_tag="ACHY09_005029"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFS3887849.1"
                     /translation="ALPALAMEVWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGKAK
                     ANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQG
                     TVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNPWRYVRA
                     ATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWKGQE
                     GSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEISQYGNPGSC
                     SVKPDNNSGAVTPVDPTPETPVTPVTPTPDNNEPSTPADSSNDYSLQAWSGQEGSEIY
                     HVIFNGNVYQNAWWVGSEDCPRGTSAENSNNPWRLVRTATAAEMSQYGNPTTCEIDNG
                     GVIVADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWSAS
                     TVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKAQSYSNEELN
                     NAPQFNPETLYASDTLIRFNGANYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGTPK
                     KAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYGMQ
                     NYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLRVLDF
                     DIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLSDA
                     KAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHSQLKGLHPNKSDA
                     EIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGTNLS
                     PEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          652 a          719 c          654 g          721 t
ORIGIN      
        1 ttaagcaaag aatagaaata tgaataaaaa taatgggcta ccaaaagtag cccattgaca
       61 aaaaatgcgg cgataccgga aggtatcgcc aacacattta ttgcttagta aacggcgcga
      121 agatttcgct aaatgcgtac ttcggtgcct gttctttagt caggccgtgg aattccggag
      181 acaggttagt gccgcctggc aggtcgcgcg cgattgacca gataccaacc ataccgagat
      241 tacgcttctg cgcatcctgc atgaccagac gagcatcaga gagatagaac acctcgccct
      301 gaacgtcgtt cacgccaacc atcggcgtgg tacccatcat agcgtcaatt tctgcatcgc
      361 tcttattggg atggaggcct ttcaattgtg aatgcaggtt ggcaatcgca gacgttgcac
      421 acttaccgtg aatgttctgg ccttcggtat ttgcagactg acaaatcgcg ttaccgtagt
      481 ccattgtcat cacgttcaca cccgccagct caacaccttt cgctttggca tcgctcagga
      541 cattcatccc ttccggcgtc aggccagtcg gcagaattgg caaggtgtac cagatagcaa
      601 tatctttgcc ctctgacttc catttatcct gcactttctt cacagcaagg ttacgacgtt
      661 caatagatgc ctgatccgca acccaggtgc cttcgatatc gaagtccagg actctgaggt
      721 tcaggttatc aacgatgtca taataatgct gcatcagatc gtctacgttc ttacaggaag
      781 cagccagcgg agcgttgtta gcaccaccga tagacagcat cacatcgccg ccagcttcac
      841 gcagagcttt gattttgctg tactgagcgt aattctgcat accgtaagcg gtaccccatg
      901 tcggcagaca ggtgttcgca tctttactca ccacaaacgc cagcgtgaag tggttgacgt
      961 tatgattctt agccagcgca gccagatccg ggatcgtatt cagcgtaaag tcaacatacg
     1021 gtgcataaac gtgtttcggc cacgctttct tcggcgtacc tacacgttct ttggttccgg
     1081 tccagtcaac aaaaacacgc cacgggttgc tgtcagaagg agaaactttc tgcactttac
     1141 tctgagaaat gtagttcgca ccgttaaagc gaatcagcgt atcgctggca taaagcgttt
     1201 ctggattaaa ctgcggcgca ttattcagct cttcgttgct atagctctga gccttaccta
     1261 acggcttcca cgggcggcta ttgctaccgg tggcgttttg gttcgcaacc agagctgggt
     1321 tgtcgctttg cgtccagaac agcgcttcat aagcctgccc atcaacaaca acgcgatcac
     1381 ctttcacgta cacggtggaa gcggaccatg ctttcgccgg ttcgtatttc ttccacgggt
     1441 tatcgccgcc cgggacaaag ccccatgcgt cttgatcgac agaggtttta taatgtgcat
     1501 cgttataatc tacgatgctg ttcgcgctgt acgctttgct ggcctggaaa ccatccgcaa
     1561 caatgacgcc gccattatca atttcacagg tagttgggtt accgtactga ctcatttccg
     1621 cagcggtagc tgtacgcacg agacgccatg ggttattgga gttttctgca ctagtgccac
     1681 gtgggcaatc ttcagaacca acccaccagg cattctgata aacattacca ttgaagataa
     1741 catggtaaat ttcgctacct tcctggccac tccacgcctg cagtgagtaa tcgttactgc
     1801 tatccgctgg tgttgatggc tcgttattat ccggagtagg agtaactgga gttaccggtg
     1861 tttccggagt tggatcaacc ggagtcacag caccgctatt attatccggc ttaacggaac
     1921 aggaacccgg attaccatac tggctgattt ctgttgccgt tgccgcgcga acataacgcc
     1981 atgggttgct ggcatcgttt tctttcgcat cgcctgggca atttgccgcg cctacccacc
     2041 aggcgttctt ataaatgccg ccgttgtaga taacgtacca tgtagaagaa ccttcctgac
     2101 ctttccaggc tacgacagca tagttgtcgt ttgacttatc agctggagct ggctgaacat
     2161 caggagccgg tttaacgtcc ggtgccggtt tcacatccgg cgcaggctgt ggtgctgaag
     2221 tacaggactg tggattactg gtttctgaga tttccgttgc cgtagcggca cgaacataac
     2281 gccatgggtt gctggcatca ttgcctttcg catcacccgg gcaattagaa gaggctaccc
     2341 accaggcatt tttatatacc gcaccattaa agacgacata ccaggtctga ccgccctgct
     2401 gtttattcca ggcaactaca gaagaattag acggaacagt gccctgtgct ggtgtagccg
     2461 aaccgccatt agccggcgta ttggatgcag gcgtatttga atttgaagaa gatgagctgc
     2521 cgctcttttc gcaggaaagt gtattgccaa actgactaat ttcagcagct gtcgcggtac
     2581 gctttaaacg ccatgggtta gttgcatcat ttgcttttgc ttttcccggg caatttgtag
     2641 aagaaaccca ccaggcattt tcataaattt tgccatcgaa aataacctga tatttattac
     2701 caccttgttg gttattccat acttccattg ccaatgctgg cagagc
//
LOCUS       DAZUCC010000367         2740 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZUCC010000367 DAZUCC010000000
VERSION     DAZUCC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.369"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(774..2711)
                     /gene="iolC"
                     /locus_tag="ACHY09_005030"
     CDS             complement(774..2711)
                     /gene="iolC"
                     /locus_tag="ACHY09_005030"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFS3887850.1"
                     /translation="MSMNNTVSSEQKTLDIICLGRVAVDLYGQQIGSRLEDMTTFAKY
                     LGGSSGNVAYGTAIQGLRSAMLARVGDEHMGRFIREELNRVGVNTQCLLTDPERLTAL
                     VILGIKDQDTFPLIFYRDNCADMALAPEDIHETFIASSRALAVTGTHLSHINTRAAVL
                     KALTYAQKHGLRRVLDIDYRPVLWGLTSLGDGETRFIASPQVTEQLQQVLHHFDLIVG
                     TEEEFHIAGGSTDTLTALRRVRQLTSAVLVCKCGALGCSVFEGNIADDWSQVQMHSGV
                     RVDILNVLGAGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPTKQEL
                     DDYLSREHAITRPDKDPHLNHLHRTTTRKQHWPELCVFAFDHRNQLVDIARATGASEF
                     TIPELKMLLLEGARQAAHEAGLLHNCGILADTTFGQKALNDVTGQGWWIGRPIEMPGS
                     YPLKLEHGNIGSQLVSWPKEHVVKCLVFYHPLDAESLRLKQEALIDEIYRACCQSGHD
                     LLLEVILPRDAADQSEHYYTQIVERFYLLGIQPDWWKLPPLSPARWQEISQHIERYDP
                     ECRGILILGLDAPESELKSGFAAAADIPWVKGFAVGRTIFGEPSRQWFSGQINDEQLI
                     AAVKQKYRTLIDYWREYRPAL"
BASE COUNT          702 a          688 c          705 g          645 t
ORIGIN      
        1 catccccgtc aatacggccc tcgctgtacg cttacagtta aagtatgttg tagagaaaag
       61 tttgtctgaa gtggcctact gaaatgaatt gcgcaggaaa gtgagacgga agatcggtca
      121 gaccgacacc agcaaaatat gatatatccg acgaccatca aaggtcataa aggcgataag
      181 gaataggtgc gcagatatcc gtcatggctc tgcaaaaaac tgtaaaatga agccgaatgt
      241 acgggggcag tctgaacccc aactgaagca tcttttttgc acaaaatcgg gagtccatgt
      301 acggatataa agctgcaacc taccgtcaat gtcagaacaa tggaagctgt gaaaaacggg
      361 atgcgttaat ataaactcca tgacagtagt ggaacgggtt aatcaagcag gattaccagc
      421 aagatgaggg agctgattca gcacaggcga cctgaccctg gccacggata accgtctgtc
      481 gcccgtctga ggtgtcgttt tttcgttctg ccacccctcg gttgagtaaa atggaaatta
      541 accgtcaagc tgcctgtagc gtaccgatca gaagattgct cagatggtac agctgttgta
      601 cgttctgcgg caacgtgacg agggaactac caatagccag acagttcaga aatatcatta
      661 atgcagatac gaaacagcac tgtcgggatg atgaggaaag taggacggtc atgaaacatc
      721 tttgaatacg ccccgagcaa gaatacagac gcgggacgta atgcagacgg atactaaagc
      781 gccggacgat attcgcgcca gtagtcgatc aatgtgcggt atttttgttt cactgctgcg
      841 atgagctgtt catcgttaat ctggccgctg aaccactgcc gtgatggctc gccaaagatg
      901 gtgcgaccta cggcgaagcc tttaacccat ggtatatccg cagcggcggc aaagccagat
      961 ttcagctcgc tttccggtgc atccagtcca agtattaaaa taccgcggca ttcgggatcg
     1021 tagcgctcaa tatgctggct gatttcttgc caacgagccg gacttaacgg tggaagcttc
     1081 caccagtcag gctggatccc cagcaggtaa aaacgttcca caatctgtgt atagtaatgc
     1141 tcgctttgat ctgctgcgtc gcgtggcaag atgacctcca gcagcagatc gtgtccagac
     1201 tggcagcagg cccggtatat ctcatcaatg agtgcttctt gcttaaggcg gagggattct
     1261 gcatccagtg ggtgatagaa gacaaggcat ttcacgacgt gctctttcgg ccaactgaca
     1321 agttgtgaac cgatattacc gtgctccagc ttcagaggat atgaccccgg catttcgata
     1381 ggacgtccaa tccaccatcc ctgaccagtc acgtcgttaa gtgctttctg accaaaagta
     1441 gtatccgcca ggatgccgca gttatgcagt aatccagcct cgtgcgccgc ctgacgtgcg
     1501 ccttccagta gtagcatctt cagctcaggg atagtgaact cgctggcacc tgtcgctctg
     1561 gcgatatcca ccagttgatt gcgatggtca aaggcgaata cacacagttc cggccagtgc
     1621 tgttttcggg ttgtcgttcg gtgaagatgg ttaaggtgag ggtctttgtc gggtctggtg
     1681 atagcgtgct cacgggagag atagtcatcc agttcctgtt tggttggcat cgcgggggcg
     1741 cagccgtggc gcgatacaac taatgcgcca catgcgttag cataacgaca agcttgctcc
     1801 caaccttcat cgttcagata gcctcgaagc agtcccgaca taaaggcgtc tcctgcaccc
     1861 agcacattta ggatgtccac acgtacaccg ctgtgcatct gcacctgaga ccagtcgtcg
     1921 gcgatgtttc cttcaaatac tgaacaccct aatgcgccgc atttgcatac caataccgca
     1981 gaggttagct gacgcacacg gcgcagggcg gtcaacgtat cagtactgcc gccggcgata
     2041 tgaaattcct cttcggtgcc aactatcaga tcaaaatggt gcaatacctg ttgaagctgt
     2101 tcagtcacct gtggtgaagc gatgaaacgt gtttcaccat cccccagcga tgtcaacccc
     2161 catagtaccg gacgataatc aatatccagc actctacgca gaccgtgttt ttgagcatag
     2221 gtcagcgctt taagcactgc ggcgcgggta ttgatgtgag aaagatgagt gccggtgacg
     2281 gccagggcac gtgaagaggc aatgaatgtt tcgtggatat cctcaggcgc cagcgccata
     2341 tccgcacagt tatctcgata aaagatcagc gggaaggtgt cctgatcttt aataccaagg
     2401 atcaccaggg ctgtcagacg ttcaggatca gtcaacaggc attgtgtatt gacgccaaca
     2461 cggtttaatt cttcgcggat gaaacgtccc atatgttcat cgcccacacg tgccagcatg
     2521 gcggaacgta gtccctgaat tgctgtgccg taagcgacgt tgcctgatga gcctcctaaa
     2581 tattttgcaa aggttgtcat gtcttccaga cgtgacccga tttgttgacc atataaatca
     2641 acggcgacac gccccagaca gataatgtcc agcgtttttt gctcactgct gactgtgttg
     2701 ttcatagaga tatcctccta cagaagaccg tgtgattttt
//
LOCUS       DAZUCC010000368         2719 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZUCC010000368 DAZUCC010000000
VERSION     DAZUCC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.370"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(197..1051)
                     /gene="rclR"
                     /locus_tag="ACHY09_005031"
     CDS             complement(197..1051)
                     /gene="rclR"
                     /locus_tag="ACHY09_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFS3887851.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1277..2602
                     /gene="rclA"
                     /locus_tag="ACHY09_005032"
     CDS             1277..2602
                     /gene="rclA"
                     /locus_tag="ACHY09_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFS3887852.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADIPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          783 a          595 c          613 g          728 t
ORIGIN      
        1 ataattacgt atcaagtaat agtcggcatt ttatttaatt aaagcaattt agtttgcgtt
       61 tttatttaaa aactgagcta gagtccatca aaaaaaatat ataaagccca aaaccaccgt
      121 tggaaataga aatagttgaa aggctcacaa ctcccggaat gcggattatc tttaccctga
      181 tttctggcgg ttttattcag ggtgcaagct gtctcaccct ttcccgatat tctcccggag
      241 tacaaccaaa ctcgcggaca aacgccttgt gaaaagatga ttcactggca tagcctactg
      301 actcagcgat caccacaaca gggagcattt cccgggaaaa catctgggcc gctatttgta
      361 gacgcaactt tgttaataca gccagcggcg tggttccgga aacatcacgg aaaagctggg
      421 caaaacttgc ccgggacatg tgggcgatgc tggccagcga ttcgacggtc caggcgtgtc
      481 ctggcatttc cagcatttgc tgtattaccg caccaagacg tggatgcaga agcaaactga
      541 gaatgttttt ctctgtatta acctgtgcaa tccactcacg caccgcgagg gtaaagaatg
      601 tagcgcagat ctggctacac agagcatcca ctcccggcat tgccgatctg gattcctgtt
      661 gtaaaaacgg gatcgcctcc ctcagccagt tatattccac gctatggttc actggtgcta
      721 caaacagcgt ttccggcaaa gatgttaaaa aataacgcgc cgaatgttgc agccgaagag
      781 tgccacagac aatacaggtc gattcattat cgacatgact caggcgatga gctgaatttt
      841 gtggtagcag gaccacattt cccgggcgta atgtaaaaat ctcccccgtc ggcatttcca
      901 gcttcgccgc tccttgcgtt aacgcatgcc aacgaataac cgacaattcc ccggcaccat
      961 gtggaagttg ccagtcactt cctaacacgc aattcttatc gatcgttcct tgtggagcgt
     1021 taagcatcaa caaacggcta agggcatcca tatcagactc ctgagtggtt tttgattaca
     1081 aacaccaaca atcttagaca ataattaatc taatatcatc aaatggttaa tcatgatttt
     1141 tgttttgtga ttctgattaa gaaaaagaaa ataccagacg ataagacaaa atatcaagcg
     1201 ttgtgaagaa atgttatttg ctcttttgcg tctatagtca tgatgtcaaa tgaacgcgtt
     1261 tcgacaggaa atcatcatga ataaatatca ggcagttatt attggttttg gcaaggctgg
     1321 aaaaacatta gccgtcacgc tggcaaaagc aggttggcgt gtggctctca tcgaacaatc
     1381 aaatgcaatg tatggcggga cctgtattaa tatcggctgt atcccaacca aaacattggt
     1441 tcatgacgca cagcagcaca cagattttgt ccgtgccata cagcgtaaaa atgaagtggt
     1501 taatttttta cgtaataaga attttcataa tcttgcggat atacccaata tcgacgtgat
     1561 cgacggccag gcggagttta tcaataatca tagcctgcgt gttcatcggc ctgggggaaa
     1621 tctggagatt catggcgaga aaatttttat taataccggt gcacaagccg tggttccgcc
     1681 aattcctgga attaccacca cgcctggtgt atatgacagc accggattac ttaatctaaa
     1741 agaattgcct gggcatttag gtattttggg cggcggatat attggcgttg agttcgcctc
     1801 tatgttcgct aattttggca gcaaagtaac cattttagag gcagcttcgc tgtttttgcc
     1861 tcgggaagat cgggatattg ctgataatat cgcgacgatt ttacgcgatc agggtgtcga
     1921 tataatcctc aatgcccatg tggagcgaat cagtcaccat gaaaatcaag tgcaagtgca
     1981 tagcgagcac gcccaactgg cggtggatgc actgttaata gcttccggtc gtcaaccagc
     2041 taccgcttcg ttacatccag aaaatgccgg tatcgcagta aacgagcgcg gggcaattgt
     2101 cgttgacaag cgattacata ccaccgcaga caatatttgg gcgatgggag atgttaccgg
     2161 cgggctgcaa tttacttaca tatcactaga tgattaccgc attgtacgtg atgagttact
     2221 gggtgaaggc aaacgtagta ctgatgatcg gaaaaatgtg ccttattccg tatttatgac
     2281 accgcccctg tccagggttg gtatgacaga agaacaagcc agagagagtg gtgctgatat
     2341 tcaggtggtg acattgcctg tagctgcaat tccgcgcgcc agagtgatga acgatacccg
     2401 tggggtatta aaagcgattg ttgataataa aacccaacgt atattagggg catcactgct
     2461 atgtgttgac tcccacgaga tgatcaatat agtgaaaatg gtgatggatg ccgggctgcc
     2521 ttatagcata ttacgcgatc agatatttac tcatccgtcg atgagcgaat cactcaatga
     2581 tctattttca ttagtcaaat aaactcaaaa tcagacgcca gaacaaatat cctggcgtct
     2641 cagagaaaag aatcttatta attctctgtt actctataaa ttcttaatca cttcattgat
     2701 ggtattttat atgtttaaa
//
LOCUS       DAZUCC010000369         2717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZUCC010000369 DAZUCC010000000
VERSION     DAZUCC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.371"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            847..1845
                     /locus_tag="ACHY09_005033"
     CDS             847..1845
                     /locus_tag="ACHY09_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFS3887853.1"
                     /translation="MKNPELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVAEYPGARWLFLTLTVRNCSIDALGETLTAMNAGFLNLKRRKEFRGVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVSPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
     gene            complement(1828..2265)
                     /locus_tag="ACHY09_005034"
     CDS             complement(1828..2265)
                     /locus_tag="ACHY09_005034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887854.1"
                     /translation="MAVAASARNALKIIRCTANKKLSSAKNHVKTIASTFLSPSITVK
                     HFASVGKLLTSIIQFLIDNNSGDIPSFEGLFLPTHHKQQPYHRQKRAQNASDGLNQPK
                     HTKPLLNAKKCVRKRLSAFLESKKLSAVDFHCRDPETRSIIAA"
     gene            complement(2508..>2717)
                     /locus_tag="ACHY09_005035"
     CDS             complement(2508..>2717)
                     /locus_tag="ACHY09_005035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887855.1"
                     /translation="SVADRTQSKRSRDCISTHFVRFPDCQFVDRAEQLRHENGRMICC
                     PVARTARFRGGRRGAKTADIAPAFW"
BASE COUNT          608 a          603 c          804 g          702 t
ORIGIN      
        1 aaaaaaaccg agaaatggcg gtggttttcg ggacaggatg tgtatttgtc ggaccgcgca
       61 cacgcaatca gacaaatgcc gcctggtcag gactgcgccc cgacaccccg ccaacgtctc
      121 acgcagccag attgaaatct gtcagcgttc gccgttttat gactcgtcaa acagaaaatc
      181 gtggggtccc gcgcctccgg cgtgggttcc gattttgtga ttgacgacat ggcggataaa
      241 aacactggtg gtggtggttg gtgtcacgca gtgaggccaa ccacctgcaa agcgggtttt
      301 taggaggatg ggaattgaaa aatatctatc agctatacga ggaaaatcgc aacagagccg
      361 gatttaaggt tcggcggaca tcatggggga gaactgttgc ggttattaaa tccattggtg
      421 gaaagactcg cgggaagctt tcgggttatg cgccttacta cggcaatcaa agggttgtgg
      481 ctgagattca tacgccgagc ggtattatta cggatgttct ttcgtgtgct gggtgctacc
      541 agtgggagcg cgtggattga tgtgttttta ggtggtcgcc accttgtata tatacttaag
      601 ggataaatcg acaactcgaa atcatgctct aggccttgtc acgactggcc tgaacgtgga
      661 tttgcgtgaa aataggtttg agagtatttt gtcggaagtg gtcgcattaa aagcgacccc
      721 cgcaacgatg ggcgtcgttc gggggtgtga ccacctgtag gaattcgaat tggtgacacg
      781 acaacacagg tgatacagga atgataacac gtaatttcgt ctgtcgctca ctagggaacg
      841 gcagacatga aaaatcctga acttaaaaat ctgacggatt attccccgga ggataaaccg
      901 tgggatgagc gccggagctt ttccgacgac gtgggcggta tttatctgcg tgctgccgga
      961 tttgagcact acggcgaacg tgtcggggct tgtagtggta ttttgcggtt tgggtggtca
     1021 acaaacacag agaccggaga aacgcgcctg cggttgcgtg atgcgcggtt ctgtcgtgtc
     1081 cggcactgtc cgatctgcca gtggaggcgc tccatgatgt ggcaagcccg tttctatcag
     1141 tccctgccaa agattgtggc ggaatatcca ggcgcgcgct ggttgtttct cacgctgacg
     1201 gtgcgcaact gttccattga tgcgcttggt gagactttga ccgcgatgaa tgcgggtttt
     1261 ttgaatctca agcgtcgcaa ggagtttcgt ggtgtgctgg gctgggttcg taccacggaa
     1321 gtaacgcgcg gcaaggatgg tagcgcacat ccccattttc acactctgct gatggtttcc
     1381 ccctcgatgt tgtccggccg tgagtatgtg aaacatgccc gttgggtgga actgtggcgt
     1441 gattgtctgc gggtggcgta tgacccgaat gtggatatca gagtggtgaa gtcacggaaa
     1501 ccaaaagacg atgaatcttt accttgtgtt gttgcggaac agctgcgcgg cgcggtggtt
     1561 gaaactctga aatattccac aaagcctgct gatatgctgg ctgatcctga atggttcctc
     1621 gaattaacac ggcaggtaca caagcgtcgt tttgttgcta cggggggagc gcttaagaac
     1681 gttctgcaac tggagcggga aacgaacgag gatttaatca tggcggatgg tatggcagag
     1741 ggtacagatg acggttcccg tctggcgttt tcgtgggtgc cggttgtcaa aaaataccag
     1801 cgcgatcggg cgaaagataa acgtcggcta agcagcgatg attgagcgcg tttctgggtc
     1861 tctgcaatgg aaatccacgg ctgacagctt tttgctctcc agaaacgcgc tcagacgctt
     1921 tctaacgcac ttttttgcat tgagcaatgg ttttgtatgt tttggttggt tcaggccgtc
     1981 agaggcgttt tgagcgcgtt tttgacgatg gtatggctgc tgtttgtggt gtgtgggtaa
     2041 aaaaagcccc tcaaaagagg ggatatcacc ggaattattg tcaatgagaa attgaattat
     2101 ggaggtgagc aacttcccga cagatgcaaa atgtttgacc gtaattgaag gagacaggaa
     2161 ggtgctcgcg attgttttaa cgtgattttt tgcagatgac aattttttgt tcgctgtaca
     2221 tctgatgatt ttgagtgcgt ttctggcgct tgctgctact gccaaaccat ccccatggtc
     2281 agttgatgcc agaaacgcgc agaagttttg tagcgcgtct ccggtcccta atcaatgaat
     2341 tcccggtcat taacatgcgt ttacccggaa acgcgccctg atgttctatc gtattgcggg
     2401 aaaggtgtcg agggtgtcgg gcaaagccct gaccaggtgg taatcgtata gccgtgcgtg
     2461 cgcggttata cgattacaca tcctgtcccg atttctgcgg gtttgtgtca ccagaacgct
     2521 ggcgcaatgt cggcagtttt tgcgccgcgc cgccctccac ggaagcgcgc agttctggca
     2581 accggacagc aaatcattct gccgttttcg tgccgtagct gttccgcgcg atcgacaaac
     2641 tggcagtcgg ggaatctcac gaagtgcgta cttatacaat ctctacttcg tttcgattgc
     2701 gtgcggtctg cgacgct
//
LOCUS       DAZUCC010000370         2689 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZUCC010000370 DAZUCC010000000
VERSION     DAZUCC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.372"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..194
                     /locus_tag="ACHY09_005036"
     CDS             <1..194
                     /locus_tag="ACHY09_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887856.1"
                     /translation="DALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGKQLIVAV
                     REQDMKKMEAEFGRIIKIH"
     gene            474..623
                     /locus_tag="ACHY09_005037"
     CDS             474..623
                     /locus_tag="ACHY09_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3887857.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            907..1167
                     /locus_tag="ACHY09_005038"
     CDS             907..1167
                     /locus_tag="ACHY09_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFS3887858.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1392..1466
                     /gene="tap"
                     /locus_tag="ACHY09_005039"
     CDS             1392..1466
                     /gene="tap"
                     /locus_tag="ACHY09_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFS3887859.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1459..2316
                     /gene="repA"
                     /locus_tag="ACHY09_005040"
     CDS             1459..2316
                     /gene="repA"
                     /locus_tag="ACHY09_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFS3887860.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
BASE COUNT          713 a          660 c          696 g          620 t
ORIGIN      
        1 gggatgcact gaacgccagt gagcatatta ttatttttaa ttgtacagga tggcctgatt
       61 tgaataatga atctgccgtc atgcagtttg ccagagtggc acgaaaatca ggaaaacaac
      121 tgatagtcgc agtaagagaa caggatatga aaaaaatgga agcagagttt ggaaggatca
      181 taaaaatcca ctgacgcctt cgcgttgctc agttgtccaa ccccggggac gggaaaaagc
      241 aagttttccc cgcccccggc gtttctgtag tgaaaagcgg gaaatggagg gaatggtcag
      301 taacagggaa tgtggtatta tcgcggcggg tgtctgagcc tttctggttc aggcaagacg
      361 caggtaccag aaatgcgaag accccacttg ttaatccatt aactcgtgag gcctgcatga
      421 agtacttgaa cactactgat tgtagcctct tccttgcaga gaggtcaaag tttatgacga
      481 aatatgccct tatcgggttg ctcgccgtgt gcgccacggt gttgtgtttt tcactgatat
      541 tcagggaacg gttatgtgag ctgaatattc acaggggaaa tacagtggtg caggtaactc
      601 tggcctacga agcacggaag taagctgccg ggcggggacg gaagtccccg ctttccggaa
      661 gtgtgaggta tttcaggggc agacacccga catgccagaa accgccggtc ccgcccgggg
      721 ccggcacaca ggttaaggca tttcctgttt ttcagccatt tcgcaacaaa aatcacatta
      781 aacaacagta atcaccgttg atttgtgcgc caacacagat cttcgtcaca attctcaagt
      841 cgctgatttc aaaaaactgt agtatcctct gcgaaacgat ccctgtttga gaattgagga
      901 ggcgagatgt cgcagacaga aaatgcagtg acttcctcat tgagtcaaaa gcggtttgtg
      961 cgcagaggta agcctatgac tgactctgag aaacaaatgg ccgctgttgc aagaaaacgt
     1021 cttacacaca aagagataaa agtttttgtc aaaaatcctc tgaaagatct catggttgag
     1081 tactgcgaga gagaggggat aacacaggct cagttcgttg agaaaatcat caaagatgaa
     1141 ctgcagagac tggacatact aaagtaaaga ctttactttg tggcgtagca tgctagatta
     1201 ctgatcgttt aaggaatttt atggcgggcc acgccgtaag gtggcaggga actggttctg
     1261 atgtggatgt acaggagcca gaaaagcaaa aaccccgata atcttcttta actttggcga
     1321 gtcagaaaga ttaccggggc catctaaaaa ccgtatagcc aacaattcag ctatgcgggg
     1381 agtatagtta tatgcccgga aaagttcaag acttctttct gtgctcgctc cttctgcgca
     1441 ttgtaagtgc aggatggtgt gactgatctt caacaaacgt attaccgcca ggtaaagaac
     1501 ccgaatccgg tgtttacacc ccgtaaaggt gccggaacgc tgaagttctg cgaaaaactg
     1561 atggaaaagg cggtgggctt cacctcccgt tttgatttcg ccattcatgt ggcgcatgcc
     1621 cgttcgcgtg gtctgcgtcg acgcatgcca ccggtgctgc gtcgacgggc tattgatgcg
     1681 ctgctgcagg ggctgtgttt ccattatgac ccgctggcca accgcgtcca gtgttccatt
     1741 accacactgg ccattgagtg cggactggcg acagagtccg gtgcaggaaa actctccatc
     1801 acccgggcca cccgggccct gacgttcctg tcagagctgg gactgattac ctaccagacg
     1861 gaatatgacc cgcttatcgg gtgctacatt ccgaccgata tcacgttcac accggcgctg
     1921 tttgccgccc ttgatgtgtc tgaggatgca gtggcagctg cgcgccgcag ccgtgtggta
     1981 tgggaaaaca gacagcgcaa aaagcagggg ctggataccc tgggtatgga tgagctgata
     2041 gcgaaagcct ggcgttttgt ccgtgagcgt ttccgcagtt accagacaga gcttaagtcc
     2101 cacggcatga aacgtgcccg tgcgcgccgc gatgcaggca gaacccgcca ggacattgtc
     2161 accctggtga aacggcagct gacgcgtgaa atcgcggaag ggcgtttccg gggaagcctg
     2221 gaggccgtga agcgcgaaat cgaccgtcgt gtgaaggaac gcatgataat gtcgcgtaac
     2281 aacaactaca cacggctggc cactgcctcc ccctgagttt tacctcatct gaataatccg
     2341 gcctgcgccg gaggcatctg cacgcctgaa gcctgtcagc gaacaaaaaa acagcaccac
     2401 atacaaaaaa caatctcatc atcccgcttc tggtgcatcc ggcccccctg ttttcgatac
     2461 aaaacatgcc tcactgacgg ggaattttgc ttatccacat ttaactgcaa gggacttccc
     2521 cataaagtta caaccgttca tgtcataaag cgccagccgc cagccttaca gggtgcaatg
     2581 tatcttttaa acacctgttt atatctcctt taaaccactt aactacattc atttaaaaag
     2641 aaaacctatt cactgcctgt cctgtggaca gacagatatg cacctccca
//
LOCUS       DAZUCC010000371         2674 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZUCC010000371 DAZUCC010000000
VERSION     DAZUCC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.373"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..221
                     /locus_tag="ACHY09_005041"
     CDS             <1..221
                     /locus_tag="ACHY09_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887861.1"
                     /translation="TIKSAGIKEPVPWDVFPELEPDTFGSLQGELEFWWESIWSPFWH
                     SLSPDERNDFLVKNNATPQWREFIEFHC"
     gene            269..>2674
                     /locus_tag="ACHY09_005042"
     CDS             269..>2674
                     /locus_tag="ACHY09_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFS3887862.1"
                     /translation="MSIPALNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQ
                     FTSTDKAITPELVLMQDGAFSLTAPPVQGMPVQAPLRTLYGVITGFKHLSSSQDEARY
                     EVRLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQV
                     MQYGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPAFIPRPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAVILLSAPKGV
                     AIASGEHLQLAAAKNVIANAGNNADVGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAKKEIIITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIEGGDYSRCFKFFDDENIPYRNVKYIANFIDGTK
                     KEGV"
BASE COUNT          682 a          696 c          778 g          518 t
ORIGIN      
        1 aaactattaa atctgctgga ataaaagaac ctgtaccatg ggatgttttt cctgagttgg
       61 aacccgatac tttcggtagc ttacaaggag aacttgagtt ctggtgggag agtatctggt
      121 ctccgttctg gcacagtcta tcacctgatg aaagaaacga tttcttagtg aaaaataatg
      181 cgacaccaca atggcgagag ttcattgagt ttcattgttg atttattttt aatgacgaaa
      241 atatttctat ctatacaggg aaataatgat gagtattcct gccctaaact ttgaccattc
      301 acaccacaaa ctcaaaatcc gcggcctgca aagtccggtg gatgtgctga ccttcgaagg
      361 ccgcgaacaa ctgagtaccc cgttccgcta cgacattcag tttaccagta ccgataaagc
      421 catcacgccg gagttggtgc tgatgcagga cggcgcattc agcctgacgg caccgccggt
      481 acagggaatg ccggtgcagg cccccctgcg caccctgtat ggcgtcatta ccggctttaa
      541 acatctgtca tcgtcacagg acgaagcccg ttacgaagta cggctggagc cgcgtatggc
      601 gctgctggcc cgcagccgcc agaatgccat ctaccagaat ctgacggtgc cgcaaatcgt
      661 cgaaaaaatc ctgcgcgagc ggcaccagat gcgcggtcag gacttcgtgt ttaacctcaa
      721 aagcgaatac ccggcccgcg aacaggtgat gcagtatggc gaggacgacc tgacgtttat
      781 ttcacgcctg ctgtcggaag tgggcatctg gttccgcttc gccaccgacg cccggctgaa
      841 aattgaggtt gtcgagtttt atgacgacca gagcggctat gagcgcgggc tgacgctgcc
      901 gcttcgtcat ccttcagggc tgtttgatgg tgaaacggaa gccgtatggg ggctgaacac
      961 cgcgtacagc gtggtggaaa aaagcgtcac cacgcgggac tacaactacc gcaccgccac
     1021 agcggagatg atgacagagc agcatgacgc caccgggggc gataacacca cctacggcga
     1081 agcctatcac tacgccgaca acttcctgca aaaaggcgat aaagaggcag cggaaagcgg
     1141 cgcgttttat gcccgtatcc gccacgaacg ctacctgaat gaacaggcca ttctcaaagg
     1201 ccagagcacc agttcactgc tgatgccggg gctggaaatc agggttcagg gcgacgacgc
     1261 gccagcggtg ttccgcaagg gcgtactgat taccggcgtg acggccagcg cggcgcgtga
     1321 ccgcagctat gaactgacgt ttaccgctat cccgtactca gaacgctacg gctaccgccc
     1381 ggcgtttatc ccccgcccgg tcatggccgg aacgctcccg gcgcgggtca ccagcacggt
     1441 gaagaacgat atctatgccc atatcgacaa agacgggcgc taccgggtca acctcgattt
     1501 tgaccgcgat acgtggaaac cgggttatga gagcctgtgg gtacgccagt cgcgcccgta
     1561 tgcgggagac acctacggcc tgcatctgcc gctgctggcg ggaacggagg tcagcatcgc
     1621 gtttgaggaa ggcaacccgg accgaccgta tatcgccggg gtaaagcacg actcggcgca
     1681 caccgaccat gtgaccatcc agaactacaa gcgtaacgtg ctgcgcaccc cagcaaacaa
     1741 caaaatccgc ctcgacgatg agcgcggcaa agagcatatc aaggtcagta ccgagtacgg
     1801 cggcaagagc cagctaaacc tcggccatct ggtggatgcc gggaaacagc agcgcggcga
     1861 aggctttgaa ctgcgtaccg atatgtgggg cgcagtgcgg gcgaagaaag gcatctttat
     1921 cagcgccgat gcgcaggaca aggctcaggg tcaggtgcgg gaaatggctc cggcaatggc
     1981 aatactggat ggcgcacagt cacagatgaa gtcgctgtcc actgatgcgc agacggcgaa
     2041 tgccgatccg gcagatctgt catcacagat agcgctgttg cagcagagtg taaaagatct
     2101 gacgcaggcg gtgatcctgc taagcgcacc caaaggcgtg gcgatcgcca gcggcgagca
     2161 cctgcaactg gcggcggcaa agaatgtgat agccaacgca ggcaacaacg ccgatgtcgg
     2221 cgtggtgaaa aatatgttta tcggcgtggg acaggcgctg agcgtgtttg tgcgtaaagc
     2281 gggcatcaag ctgtttgcca ataagggcgc aatctcggtg caggcgcaga acgacttgat
     2341 ggaactgttg gcgaagaagg aaatcatcat taccagtacc gaagatgaaa ttaaaatcac
     2401 ggcgaagaaa aagatcaccc ttaacggcgg tggcagctac atccggctgg atgcctgtgg
     2461 gatagaagcg ggaacgccgg gagaatataa cgttaaggcg ggatattatg ggcgtaagcc
     2521 gaaggcgaag ctgacgccgg agctgatggc gtttccagtg attgagggtg gagactattc
     2581 tcgctgtttt aaattttttg atgacgaaaa tatcccatat cgtaatgtta agtatatagc
     2641 caattttatt gatggaacaa aaaaggaagg tgtc
//
LOCUS       DAZUCC010000372         2663 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZUCC010000372 DAZUCC010000000
VERSION     DAZUCC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.374"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            180..629
                     /gene="glmS"
                     /locus_tag="ACHY09_005043"
     CDS             180..629
                     /gene="glmS"
                     /locus_tag="ACHY09_005043"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFS3887863.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            629..2017
                     /gene="glmL"
                     /locus_tag="ACHY09_005044"
     CDS             629..2017
                     /gene="glmL"
                     /locus_tag="ACHY09_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFS3887864.1"
                     /translation="MQIVSVDIGSTWTKAALFAREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLDQVLNVDDALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQSYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     VVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSASANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            2036..2521
                     /locus_tag="ACHY09_005045"
                     /pseudo
     CDS             2036..2521
                     /locus_tag="ACHY09_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="methylaspartate mutase subunit E; catalyzes the
                     formation of threo-beta-methyl-L-aspartate from
                     L-glutamate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
BASE COUNT          731 a          656 c          664 g          612 t
ORIGIN      
        1 tgagaatttg aaaacacact ttaaacaatc atttcccgaa agacattttg aaaatagatc
       61 aattgcaaca ttacggtcag ataaagacac tcctgtgata ttaaacctgt gccgttatga
      121 gcggccataa atacgacgtg tgtaacttat tttactctct gtcttacaaa ggattaccca
      181 tgaagaaagc aacacttgtg attggcgtta ttggcgcgga ctgccatgca gtaggcaata
      241 aagttctgga ccgcgttttt agcaaccatg acttcagagt tattaatctg ggcgtaatgg
      301 tcagccagga cgaatatatt gatgctgcca ttgaaactgg tgctgacgcg attgtcgtct
      361 cctctatcta tgggcatggc gatatcgact gtctgggcat gcgcgaacgc tgcatcgagc
      421 gtggactggg ggacatcctg ctctatgtgg gcggtaacct ggtggtcgga aaacatgact
      481 tcgctgacgt ggaaaccaaa ttcaaagaga tgggatttga tcgcgtcttt gccccaagtc
      541 atgacttaga agatgtttgt cagctgatgg cccacgatat caaccaacgt catgacgttg
      601 atactcgtat tcttgaagag gcaatctgat gcaaatcgtc tctgtcgata ttggatcgac
      661 gtggaccaaa gcagccctct tcgcccggga gggggacgcg ttaacgttgg ttaaccacgt
      721 actgacccca accaccaccc atcatctggc gaaggggttt ttctccagcc tggaccaggt
      781 gttaaacgta gatgatgccc tccccctgtt aaacagtggt gaggttgcat tgaagtattc
      841 ctcctctgcc aaaggtgggc tggcggttgc cgcaatggga ttggtgcctt ctatcaccct
      901 ggaaaccgcg aaagtaaccg ctcactctgc gggtgctaaa attgcgcaat cttacgccta
      961 caagctgaac cgccgtgata tccaggcact ggaagaaacg cagcccgata ttctcttgtt
     1021 caccggcggc accgatggcg gagaagaaag ctacggactg aataacgccc gtgtgttggc
     1081 agaatccaaa ctcgactgcg ccattattta tgccggaaac cgggatattc aggacgaggt
     1141 gcaggagatc ctcgggcata aagacctgac catcgtcgac aacgttctgc ccgatcttga
     1201 ccatccgaac cctttagctg cacgccaggc tatctgcgac atattcctta aacgcatcgt
     1261 taaagggaaa gggctggatg tcgttgttga taaaacaggc gaagaaccaa tgccgacacc
     1321 gtggacagtc tttgaactgg ttaaggcaat cagtaatgtc gatcacagct ggaaagagtt
     1381 catgctgatc gacatgggtg gcgcaaccac cgatgtctat tccgccagtg ccaacacgct
     1441 ctcgcccgac actgttttac atggcgtacc ggaacccttc gtaaaacgga cggtggaagg
     1501 agatctggga atgcgcgtct cggcaatggt ggttggtgaa agtgcgaaag agttagtcaa
     1561 tgtgaatttt gctcagcaac ccgcacagct ggacgctttt tatcattacc tccgtcatct
     1621 agtcgctcat cccgactact tacccgcgaa tgcagaagaa aaatatttcg ataccgtcct
     1681 ggccggactg tgtgtgggtt atgccaccga aagacatgcg ggcaccaaaa aagaggtctg
     1741 cacctgcgtc ggcaatgtcg atttacagat gggacgtgac ctgacaaccg tgcgtaaagt
     1801 cgtcggatcc ggcggatggc tctcgcgtgc cagtcagttc gatatgcatc actggttgaa
     1861 ataccgcgag ttaaacgacg acggtaaacg cattctctta ccgaccgaat ttgaatatta
     1921 ccgcgattcc cgggggctat taccactgct ggcaaacgtc gccagagtcg atccgctggc
     1981 cgccgcgcgc accagtattc aatgcttaac cctataacaa ttaaggctgc aatcaatgga
     2041 acttcgaaat aagaaattaa cccatgacga attcatgacc gagcggcaac aggtactgaa
     2101 aacctgggaa accggcaagg atgttgaaaa ctttgaagat ggcgttaaat accaacaaac
     2161 cattcctgag cataaacgct tctctcttgc gctgttgaaa gcagacaagg aaggtaaaac
     2221 ccttagccaa ccgcgcgcag gtgtggcgct gatggacgaa cacatcgaac tactgaaaac
     2281 actacaagaa gagtgcgatc tgctgccgag caccatcgat gcatataccc gtctgaatcg
     2341 gtacgaagaa gcggctgtag ggattaaaaa atccatcgaa gccggaacct ctaaactgaa
     2401 cggtttaccg gtcgttaacc acggcgttgc agcttgccgt cgcctcaccg aaactctgca
     2461 aaaaccgcta cagatccgcc acggcacgcc agatgcgcgt ctgctggctg aaatctcgat
     2521 gtgtatacat gaatatactc taaataagtc aagttgcagg aagggaacaa ataatgaatt
     2581 tccagaagct tacataagta agtgacctgg gtgagcgaac atcgatgcag cacatgcaac
     2641 ttgaattatg acaagtatac aat
//
LOCUS       DAZUCC010000373         2641 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZUCC010000373 DAZUCC010000000
VERSION     DAZUCC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.375"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(363..944)
                     /locus_tag="ACHY09_005046"
     CDS             complement(363..944)
                     /locus_tag="ACHY09_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS3887865.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRAVIHIVPRLAGAGGNGVFQVVLGAAAIVGSFFTGGATMALWGAALSAGGL
                     TATTMLFSLGVSMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            complement(941..1684)
                     /locus_tag="ACHY09_005047"
     CDS             complement(941..1684)
                     /locus_tag="ACHY09_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS3887866.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHR
                     FRCVPHLTVRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLADN
                     GFYRVSPSCAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            complement(1695..2393)
                     /locus_tag="ACHY09_005048"
     CDS             complement(1695..2393)
                     /locus_tag="ACHY09_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS3887867.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSALTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
BASE COUNT          611 a          843 c          612 g          575 t
ORIGIN      
        1 ttcagccccg caacagaaaa tttctgaaat tattcacatt ttcgcaaaat ccatgtagcg
       61 cttataattt cgctgcgtta atgtttttct gccgtgatat aaaaataatt ccttacagtt
      121 aatcttcgtc tctcttcccg cagtctccgg actctgcggg atttttttat cccgtcactg
      181 ggcagcccgg gcatcaccac aacggaataa aaattctgag tttattcaca cttccggcaa
      241 ttgcctgtag ccgcaataat gacgctgcgt tactgttctt ctgcccaaaa aaataactcc
      301 ataacgttaa tcttcatcgt tatttcccgc agctccgcta actctgcggg atttttttat
      361 ttttatcccc gcccgataac caccactttc ccgtcaccgc cctcatcacg ggtgctgatg
      421 tcctgggata tccgtcgtga accaaccagc atttcaccgt aaggcaccgg catcgggtta
      481 ccctgggcaa tcatgttatc cagtgacgaa aaatacgtgt tctgtttacc gttatccgtg
      541 cttttgtact ccggcgtctt tgccttcggg gccagcatct gtgccacgcc acccagtatc
      601 atgctgaccc ccagtgagaa cagcatcgtg gtggcagtca gccctccggc actcagggct
      661 gcgccccaca acgccatcgt tgcgccgccg gtgaagaaag agcccacgat ggctgctgcc
      721 cccagcacca cctgaaaaac accatttccc ccggctccgg ccagccgcgg tacaatatga
      781 atcacggccc tcccgctcag tggctcatga agacgggcat acaccgcctc cggcgcggta
      841 tcctcaccgg caatacgtat ctgataccag ccttcgttca tctgacagcg gaatcccggc
      901 acctgcagcg acagggcacg gatggcctcc gctgccgtgt tcacatacag gctgatgcgg
      961 cgaccaaatc gttgtaaatc cccgtgaagg cagatgcgga ccagtggcgg tgacgccaga
     1021 cagaatgcgt tcgtcgttgc catttttcag aatacctctc ccgtttactc agttgttcag
     1081 gtatatggtg aagcagttca ccgttgccac agtaaatggc ggcatgattc ggcaccgatg
     1141 cgccaaagca gcacagcagg atatcgcccg cctgtgcaca ggacggagac acccggtaaa
     1201 agccgttgtc cgccaggttg tccaggtaca ggttctggcc gttgcgccac cagtcatcct
     1261 cacgcacaaa atccggcagc gttatccccg ccagatggta tgcatcccgg aacagggtgt
     1321 aacagtccgt cacaccgtgt tcaaagcgcc gtacggtcag gtgcggcaca cagcggaacc
     1381 ggtgaatgtc accccggcag accagccacc agggcagggc actctttatc tgcagcctcc
     1441 ggtccgcctc gctcagccag ggcagaccac cgggatgact gtggaccagc gccacaatct
     1501 ccccctgcat ctctgcccgc agccagtctt ccggtgcgat acgaaaatac gcctccggct
     1561 ctgcggagat attcacgcag ggctggtacc gttcgccctc cggggtgctt atcacgaagc
     1621 cgcacgactc cgctggcgca caccgccggg catgcaccag aatcgctgat tcagtctgtg
     1681 tcataaaacg ggatttactg cgaaagttta ttaatggaaa ggaaaccgcc gaaattagcc
     1741 accatgccgc gcatctcaca cccgcgcatg catttactgc atctgtcctt ccggatatcc
     1801 gtggtagggt tgtcgaactc atccgccact gccggaccgt tatacccgca ctcatcgccc
     1861 cggtaatccc acatacaggt gttcgccagc atgatacgac cgggaaacag cgccccgtcc
     1921 gtctcggtcg gtgtcgccag cacaaacgag gccgtcatgg ccgtcagcgc tgacatctgc
     1981 tccaccaccc accggtccgt cagctcctgc tccgggtctg cctcaggatt gcctgccaca
     2041 aaattcaccg catccagaaa acgcgcatac acccggcggc ggaccaccgt ggcacccacc
     2101 aggctctgca aatcctccgc catcccggtg acaaggccga aaagattgga gaccgtcagc
     2161 gacgggcggg cactgctgcc ctttccgttc atctcaaagc cgctgccctc aatcgggtac
     2221 gcctgatatt cacgcccctg ccaggtcacc ggctcccctt tttcattcag ctcattgcag
     2281 aaaaaatacc gctcaccgcc ctgcaccgtc aggtcaattt cccagagcac cacccgtggt
     2341 gactgctctg acttaaccga ctcgttcagg ctttcttcgt gaatatcctg catatatccg
     2401 cccataaaaa aggggcgcag acgcgccccg aacaataaaa gtccaattta aaataaaacc
     2461 aataaaggta ataacagaaa gttaaatcag cacatgccag atggcaattg ctgatcgcag
     2521 tgaccaatga aaactgtgtt ttcattgata caggccgcct gtaagggcag acggcccgta
     2581 tttattaact tatcagcaat aatcagaact gataagtcat acccacagca acgatattat
     2641 c
//
LOCUS       DAZUCC010000374         2637 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZUCC010000374 DAZUCC010000000
VERSION     DAZUCC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.376"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..685
                     /locus_tag="ACHY09_005049"
     CDS             <1..685
                     /locus_tag="ACHY09_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3887868.1"
                     /translation="NYSTNYPADRSKADYWSSGTEGYIKIRLDKKIISDVSLNNVLLV
                     SLYASQRQTEHGPVPLFNAWISNLDIQVPQGCTINEGTSFTVNMPDVWASELSRAGAG
                     AKPAGVTPVATTIPINCTNKDTDAVMTLVFDGNISATRDTNGKQSIIQAQDNPDVGIM
                     IMDSQQNSVDLNALATSVGVPFRLVENQAASAQTADVTFLALPVSTTGKVPAAGRYNA
                     LAVLRVEYQ"
     gene            859..1569
                     /locus_tag="ACHY09_005050"
     CDS             859..1569
                     /locus_tag="ACHY09_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887869.1"
                     /translation="MELVDTLFASLSGTDPFTGVDITIANCKSTYWDEGIIQQLINQV
                     LDEGEKFAGTVGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATIGRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDFTVHFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKEAW
                     KFHTDNSLRGFRDKQAELKLYARHYSVENCTEESCRDCNYCIRPSFSLSRSSIIRLNA
                     ANARFVYQPFTRDQRKRG"
     gene            1572..2348
                     /locus_tag="ACHY09_005051"
     CDS             1572..2348
                     /locus_tag="ACHY09_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887870.1"
                     /translation="MQPSSAMQALIEQVYHIFRRYPVPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDERAILQRFACQYMADWVSGDEAVELQYMLEMFSRADIALSPLLDAIIS
                     VPGYQPTVSMACLLWHNREENIQNSRFEQDDDDKAITAQINAWAFNNQFILKERARQA
                     IENLLNQPEQGTHYQVWEDDWMIDECLCAMYDASSESPGK"
     gene            2351..>2637
                     /locus_tag="ACHY09_005052"
     CDS             2351..>2637
                     /locus_tag="ACHY09_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887871.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMGGIGWQFSRRTPPELKNRLHYWL"
BASE COUNT          722 a          528 c          613 g          774 t
ORIGIN      
        1 gaactatagt actaattatc ctgctgacag aagtaaagca gattactggt cttcaggaac
       61 agaaggctat attaaaatta ggttggataa aaaaatcatc tctgatgtct ctctgaataa
      121 tgtactttta gtttcattgt atgccagtca gcggcaaacg gaacatgggc cagtaccatt
      181 atttaatgcc tggatcagta acctggatat tcaggttccg caaggttgca ctattaatga
      241 gggtactagt tttactgtta atatgccgga tgtgtgggcc agtgaattga gccgggctgg
      301 tgctggagcg aagcctgctg gtgttactcc tgtagcaaca actattccga ttaattgtac
      361 gaataaagat acagatgcgg taatgacgtt ggtattcgac ggtaacattt cagctacacg
      421 tgataccaat gggaaacaaa gtattattca ggcacaagat aatcctgatg ttggtattat
      481 gattatggat agtcagcaaa actccgtaga tttaaatgct ctggcaacat cagtaggcgt
      541 tccgttcaga ttggtggaaa accaggctgc ttctgcgcaa actgctgatg ttactttcct
      601 ggctttacct gttagtacaa ctggaaaagt gcctgcggca ggtcgttata atgcactggc
      661 tgtattgcga gtggaatatc agtaattttt gagctagtac agaatcggtt tagccatcac
      721 cgcaaaataa aatgcaataa tagggatgaa cagaatgtaa aacaaaaaaa tatttttaat
      781 tctacaggga caaaatctgg tggaaaatgt aggttatgtg ccgatggata aaatgttgca
      841 ttaagccgga taataaaaat ggaattagtt gatacgttat ttgcaagcct ctccggtact
      901 gatccattca ctggcgtgga tattacgatt gccaactgca aatcaacata ctgggatgaa
      961 ggcattattc agcaactcat taaccaggtc ctggatgaag gggaaaagtt cgccggtact
     1021 gtcggtcttg agggattatc tcgatatgat gtcacattaa atattggctt aacctccagc
     1081 aatgtatggc cgggattttc cctcgatacc gccacgatcg gtcgtctttg tgcctgtggt
     1141 gctgattttg gatttgaccc ctatatcagt gatgtaccag atgttcaatg tgacctgaat
     1201 acgacaaatg actttaccgt tcattttacg gcaatgctca atccggatga gcgggtgatc
     1261 atagcaaagc gcccgcttaa aaaatgtgac tcctggattg aggatgttta tatctttcag
     1321 gtgtttaaag aagcatggaa attccatacc gataacagct tacgcggttt tcgggataag
     1381 caggctgaac tgaaactcta cgcgcgtcat tactcagtgg aaaattgcac agaggaaagc
     1441 tgtcgggatt gtaattattg cattcggccc agcttttccc tctctcgtag ttccattatt
     1501 cgcctgaatg cggcgaatgc tcgttttgtt tatcaaccgt ttacacgcga ccagcggaag
     1561 agggggtaat tatgcaaccc agctccgcca tgcaggcatt aattgagcag gtttatcata
     1621 tttttcgtcg ttatccggtg ccgcagcaat ttgtggtttg ttgtgaatat tgcctgagtc
     1681 aacaggaaca aaaagcatta cggaatacat cgttgcgagc gattccttat tccttaatca
     1741 atgcctggaa tagtagtcca ggacctgatc cgcaaaacag cgatgaggtt cgttattttc
     1801 ttcctcgttt actggagttt gttgcgcagg ggcaatttga caatattcac gaagtatttt
     1861 ctctacggcg tatcaacctt gccagtaaag aaaactggcg tgaggatgaa cgggcaatat
     1921 tgcagcgatt tgcctgccag tatatggctg attgggtatc tggcgatgag gctgtcgaat
     1981 tacagtatat gctggaaatg ttttcccgtg ccgatatcgc tctttcgcca ttgcttgatg
     2041 ccatcatctc tgtacccggt taccaaccca cagtatcgat ggcatgtttg ctgtggcata
     2101 acagggaaga gaatattcag aatagtcgtt ttgagcagga tgatgacgat aaagccatta
     2161 ccgcccaaat caatgcctgg gccttcaaca atcaattcat cctgaaagaa cgtgcgcggc
     2221 aggcgattga gaacctgctt aatcagcccg aacagggcac acattatcag gtctgggaag
     2281 acgactggat gattgatgag tgtttatgcg cgatgtatga cgcatcatcc gaatcaccag
     2341 gaaaataaaa atgattaaat tccggcttta tattccccct gtaattctcg gttttgttat
     2401 cgtgccatta ttggtatggc cgacggttat tgccttagcc gtacttatat tcacgttaac
     2461 ttttctggct gaaataatat tctcctttcc gctcctggtt gtgcgtattt ctcttcagga
     2521 attacaactt gagttaatgg ttgaatatgc actttttttc agtgtaatgg gtggcatcgg
     2581 ttggcaattc tcccgcagaa cgcctcctga attaaaaaac agactgcatt actggct
//
LOCUS       DAZUCC010000375         2512 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZUCC010000375 DAZUCC010000000
VERSION     DAZUCC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.377"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            358..501
                     /locus_tag="ACHY09_005053"
     CDS             358..501
                     /locus_tag="ACHY09_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFS3887872.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            514..723
                     /gene="cspE"
                     /locus_tag="ACHY09_005054"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             514..723
                     /gene="cspE"
                     /locus_tag="ACHY09_005054"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFS3887873.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            889..1698
                     /gene="rlmA"
                     /locus_tag="ACHY09_005055"
     CDS             889..1698
                     /gene="rlmA"
                     /locus_tag="ACHY09_005055"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFS3887874.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1695..2261)
                     /gene="mntP"
                     /locus_tag="ACHY09_005056"
     CDS             complement(1695..2261)
                     /gene="mntP"
                     /locus_tag="ACHY09_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFS3887875.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     regulatory      complement(2393..2483)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT          660 a          592 c          620 g          640 t
ORIGIN      
        1 ggggtaattg gtggccgtta actgactgtt ttatgagaaa aagtgatata actttttatt
       61 cattgcatag cagaaaatgt gatattgcac gcactatgta ataacttctc ccactggcct
      121 ggaacaactg aacttattga actatgttag aaaatacgtc agtttaagta tctgcctgaa
      181 ctggcaaggt taagcacaat gatatatcgg cgcgtattcc gttgcataag tgtgcaaaaa
      241 aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac atgtttaaaa atggcttgcc
      301 ataattaacg ttgtatgtga taacagattt cgggttaaac gaggtacagt tctgtttatg
      361 tgtggcattt tcagtaaaga agtcctgagt aaacacgttg acgttgaata ccgcttctct
      421 gccgagcctt atattggtgc ctcatgcagt aatgtgtcag ttttatctat gttatgcctg
      481 cgggcgaaga aaacaatcta aggaattttt caaatggcaa agattaaagg tcaggttaag
      541 tggttcaacg agtctaaagg ttttggcttc attactccgg ctgatggcag caaagacgtg
      601 ttcgtacact tctctgctat ccagggtaat ggtttcaaaa ctctggctga aggtcagaac
      661 gttgagttcg aaattcagga cggtcagaaa ggtccggcag ctgttaacgt aacagctatc
      721 tgatcgaatc cactgatctg aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg
      781 aatgcgaggc tttttacttt gctttatctt cgctcctggc gttcggatat ttgcccgccg
      841 cgtgattcgc gttacacttg cggcctttag tatcctgccg gagttgtcat gtcgttttcc
      901 tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca gctatatctg tccccagcga
      961 catcagtttg atatggcgaa agaagggtat gtcaatctgc tgcccgttca gcataaacgg
     1021 tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac gccgcgcatt cttagatgcc
     1081 ggacattatc agccgctgcg tgatgcaatt gtcgcccaac tgcgggaacg gcttgatgaa
     1141 aaggccacag cggtgctgga tattggctgt ggtgaagggt attacacaca cgcatttgcc
     1201 gatgcgctgc ccgaaatcac cacgtttggc ctggatgtct cgaaggtagc gataaaagcg
     1261 gcggcgaaac gctatccgca ggtcactttt tgtgttgctt ccagccaccg tttgccgttt
     1321 tccgatacta gtatggacgc cataatacgt atttacgcgc cgtgtaaagc agaagaatta
     1381 gcgcgagtag tgaagcctgg cggctgggtc attactgcca cgccgggacc gcgacatttg
     1441 atggagctga aggggctgat ttacaatgaa gtacatcttc atgcaccaca tgcagaacaa
     1501 ctggaaggtt ttacattaca gcagagtgat gagttgtgtt atccgatgcg tcttcgcggt
     1561 gatgaagccg tcgcattatt gcagatgacg ccgtttgcct ggcgtgcgaa gccagaagtc
     1621 tggcaaacac tggcagcaaa agaagtgttc gactgccaga cggactttaa tattcacctc
     1681 tggcagcgtt cttattaacc gtggaagtgc gtccagagga tctggacgcc gatgccgatc
     1741 agcaccagcc cgccgagaat ttccgctttt ttcccaataa ttgagccgat aaagcgacca
     1801 accatcatcc ctaatgtcga cataatcaag gttgcacaac caatggccaa tgcggtcgcg
     1861 ataatgttga cctgcaggaa ggcgagacca acacccacag ccatggcatc caggctggtg
     1921 gcaatcgcgg tggttaccag tagccagaaa ccgtgccgac ggcgcggctc ttcatcttca
     1981 tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc cgaggaatat cagcagcaca
     2041 aacgcaatcc agtgattcca ttcaaggaca aaccggctgg ctaacatgcc cattccccag
     2101 ccgatcagcg gtgtcagggt ttcgacggca ccaaaaataa ggccggttcg caatgcttca
     2161 gaaaatttcg gtttatggag ggtggcacct ttaccgattg atgcagcaaa tgcatccatc
     2221 gacataccaa acgcaagaag aacagtagca gtgatattca tgacaacgtc ctgaccgggg
     2281 tatccataaa acacatcatc gcccccagca aacatgcata catgaacaac aagccgcgat
     2341 gggttttcgc gttcatgcag gcatagcgat tgatgtgcat atggtctcgc ctgactgaaa
     2401 gtgttcagtc cgtacgtgcc acgttttgca acgagtatgt tgacacgtac atttccggaa
     2461 tctggaaatc ggctactccc caaatgaggg cgcaacctta acatattttg ag
//
LOCUS       DAZUCC010000376         2479 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZUCC010000376 DAZUCC010000000
VERSION     DAZUCC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.378"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..317
                     /locus_tag="ACHY09_005057"
     CDS             <1..317
                     /locus_tag="ACHY09_005057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887876.1"
                     /translation="LLNYHIACPFPAAQIRFPAFLPRRSENPESRIFLSLTQRHLLSV
                     QHVVLHSAAHDTIYCVISLTCDASRRGGKQRYDYFPSVVHFRKVVIFRKVGSDGNGSP
                     VI"
     gene            461..874
                     /locus_tag="ACHY09_005058"
     CDS             461..874
                     /locus_tag="ACHY09_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887877.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLDGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            876..1655
                     /locus_tag="ACHY09_005059"
     CDS             876..1655
                     /locus_tag="ACHY09_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS3887878.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            1835..2479
                     /locus_tag="ACHY09_005060"
     CDS             1835..2479
                     /locus_tag="ACHY09_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS3887879.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
BASE COUNT          597 a          601 c          670 g          611 t
ORIGIN      
        1 tgttgcttaa ttatcatatc gcctgcccgt ttcccgccgc gcagatccgg tttccggctt
       61 ttctgccgcg cagatccgaa aaccctgagt cacggatctt cctctccctc acacaacgcc
      121 atctcctgtc agtacaacat gtcgtgctgc attcagctgc tcatgacact atatattgtg
      181 ttatttccct gacctgtgat gcgtcgcgca ggggcggaaa acagcgatat gattatttcc
      241 ccagcgtggt acacttccgg aaagttgtga tattcaggaa agtcggatct gacggaaacg
      301 gctctccggt aatttaacgg cgtggttata tggatgcttg ttatcatggt gatgatgata
      361 acggcatgat gttatcagac ggcgtgacgg taagggcagt gatgatggat gacgttatcg
      421 catgaccgtc cctgcccgga aaagaaaaaa ggagtcaccc atgtttttta ttgagaatga
      481 aggtcaggct gtcgccagaa cggattactg gcagtctgta caggcgcagg ccggatatgt
      541 ctacctcagc tggaatgccg gcgcagccag gctgcttgtc ccggatgcgg caaaacattt
      601 actcagggag atgcgggggg ctgagtacgt catcatcagt aagggaacgc tggatggccg
      661 cgatgcgctg gaactgatat ttgaagacgg cagcgatgcg ccgtttgtga tccacatgct
      721 gagtgagcag tgcgatcgcc tgctccccga aaacaaccag ggagggggtt ttgttgtcac
      781 cgtctggacg cgtggcggta accagctccg ttatccggga aaataccggg ttgtggaaaa
      841 cctgcccgac gtttccccgt ggagtgagca ctgatatgca gcacctgccg gcaccgatcc
      901 accatgcccg ggatgccgct cagcttcctg ttgccatcga ttatccggca gcgctggcac
      961 tccgccagat gtcgatggtt catgatgaac tgccaaaata cctgctggcc cctgaagtga
     1021 gcgccctgct ccattacgtg ccggatctgc gccgcaagat gctgctggcc acactgtgga
     1081 acaccggtgc gcgcattaat gaagcactgg cgctgacgcg gggggatttt tcgcttgcgc
     1141 ctccgtatcc gtttgtgcag cttgcgaccc tgaaacaacg gacggaaaaa gccgccagga
     1201 cggcaggaag aatgcccgcc ggtcagcaga ctcaccggct ggttccgctc tccgacgcct
     1261 ggtacgtcag ccagctgcag acgatggtgg ccacactgaa aatccccatg gaacggcgta
     1321 ataaacgaac aggcaggaca gagaaagcgc ggatctggga agtgacggac agaacggtca
     1381 ggacctggat tggggaggcg gttgccgccg ctgctgctga cggtgtgacg ttctctgtcc
     1441 cggtcacgcc acatacgttc cgccattcct atgcgatgca catgctgtat gccggtatac
     1501 cgctgaaggt cctgcagagc ctgatggggc ataagtccat cagctcaaca gaggtctaca
     1561 cgaaggtgtt tgcactggat gtggctgcac ggcaccgggt gcagttttcg atgcctgagt
     1621 ccgatgcggt gtctatgctc aaaagaattc cttaagctct gactttttta aatccctttc
     1681 ggatatctca tggatatatt atcggtattt catattctgt tgacatgcag gaccggtata
     1741 gagtaaaaaa acatctattg agtacctctt ggatatccga aaggtgtctt agggggtctt
     1801 tatgggtatt acatgtggat tctggagata cactatgcct gttattgcta atacgcatcc
     1861 caaaggtggt gtgggtaaga caacttcatc cgtaaacatt gttggtgaaa tgaagtctga
     1921 tacagttgat ctggatactc ataccgggct ttctatcatt ctggggctga gacctgaagg
     1981 aaaagaaatt tccgtgaaag tacccaaaac agtggatgag ttaatcgaaa ttatgactcc
     2041 ctacaagaac agcgataaga cgttacttat tgactgtggg ggatttgact cagatcttac
     2101 ccgtactgcg attgcctttg cagactgcgt tattgtccct tcaaaagact ccctgactga
     2161 acgtattggt ctgatgcatt ttgatggagt actggacgaa atcagctcta tcatggggac
     2221 cgatataact gctcacctgt atctctgtaa agttaatcct aacaagaaga aattccctaa
     2281 gcttgatgca atcctgccat cgttcaaaca tctgaagctg atgaagagcc gcatttctgc
     2341 tcgtgcagaa tttgaggatg tcattgagac tgggatggga atcactgagt ctgttcatgg
     2401 gcgctattcg gcaggaggta aggaagttat tgccctgatc gaagaaatca atcatctgat
     2461 tgaaaataac aaacagtaa
//
LOCUS       DAZUCC010000377         2467 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZUCC010000377 DAZUCC010000000
VERSION     DAZUCC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.379"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..588
                     /locus_tag="ACHY09_005061"
     CDS             <1..588
                     /locus_tag="ACHY09_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HFS3887880.1"
                     /translation="PENLPPVPERSDIIIKTDDPVPCDGIWEPVKIHYDHKLLIIKDG
                     ISGFNNLGAYNYFIRGMNAPRQVYYDVLIESDTEMVVPDDPIRYRDVHWRLVWEDTRY
                     SDGIIPDESEYFLDDAPGKRITCPSGELCPHSGHWATIAGGHQQFIDVQAGQLMPEAT
                     KYQSNMYADEIHLPAIWNLLRRDDEGSVYVKTGES"
     gene            666..1088
                     /locus_tag="ACHY09_005062"
     CDS             666..1088
                     /locus_tag="ACHY09_005062"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFS3887881.1"
                     /translation="MDGQRYFGESLSVQQPAAVPLFDTWPDSRIAFAGPWVMEMNSIT
                     DFRERLCELEAALPSVSWMISSSSLTELAAHFRRNMNTELPDGRIALLRFHDPRVQKR
                     LGEMLNDQQHRELTGLMQEWLTIVDGKAWSFKQREFIC"
     gene            1082..1426
                     /locus_tag="ACHY09_005063"
     CDS             1082..1426
                     /locus_tag="ACHY09_005063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887882.1"
                     /translation="MLKLSEEQCVGMDKREDDDFVRDTALRLKKKFPGITESDDTFHV
                     RLRKALDYANTLPLKEKNVKRDFLFLEAFWPGFYLKPEVDKWLRTPNAYSTDQRVEDY
                     KHVMINRERREL"
     gene            1426..2190
                     /locus_tag="ACHY09_005064"
     CDS             1426..2190
                     /locus_tag="ACHY09_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tox-REase-5 domain-containing protein"
                     /protein_id="HFS3887883.1"
                     /translation="MVMVLTGAGMGSAATGGVGMAGASTGTAVFPGAGSRDGDWEEDN
                     ISYPGSGSGSRQGNGGFYYQGKQYDPILNEPMVVSAETHGDFWEDDMDHSETVNYEGI
                     DSKAEVQAKTDEKTCENCPPEGKVMPMIRRCSRWSMVTISYQTRICGTFYNPETQQIQ
                     EFKYCGVSFDGWKDKLCQFWEAKARYDQFFRADGSKKPWWTGNLSAINQASRHQAVAT
                     VNQPLKVVWIFMQPVSCEYFRKMFKDYKDIITRWQP"
     gene            2178..>2467
                     /locus_tag="ACHY09_005065"
     CDS             2178..>2467
                     /locus_tag="ACHY09_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887884.1"
                     /translation="MATVIELFNTSINIHSLKERDITIDDCLNELYRSTVVVKNLTQH
                     DHIWYLTGYSRKDASKHVVFSDNEPNQNTIDTFERHYKKNRPLINESIWNGQP"
BASE COUNT          723 a          471 c          647 g          626 t
ORIGIN      
        1 ccagaaaatc tcccccctgt tccagaacgc agcgatatta ttattaaaac cgatgacccg
       61 gttccctgtg acggcatctg ggaaccggta aagattcatt acgaccataa actattgatt
      121 attaaagatg gtatcagcgg atttaataac ctcggtgctt ataattattt tattcgtgga
      181 atgaatgctc ctcgccaggt gtattacgac gtattaattg aaagtgacac agaaatggtc
      241 gtacccgatg acccaatccg ttaccgggac gttcactggc gtctggtctg ggaagacacg
      301 cgctacagtg acggaattat tcccgatgag tcagaatact ttctggatga tgcaccaggc
      361 aaacgcatta cctgcccgag tggggagctt tgcccgcatt caggtcattg ggcgaccata
      421 gccggaggac atcagcagtt tattgatgtt caggccgggc agttgatgcc agaagcaact
      481 aaatatcaga gcaatatgta tgcagatgaa atccatcttc ctgcgatatg gaacctttta
      541 cgccgggatg atgaggggag tgtttatgta aaaacagggg aaagttaaat aactgatcaa
      601 cgctttttga cggacagatc tgttaccacc tgatttgctc aacaaaagtg acattatttg
      661 ctctggtgga tggtcagcgg tatttcggtg agagcctgtc tgttcaacaa cctgccgccg
      721 tgcccctgtt cgatacctgg cctgattcgc gtattgcatt tgcggggcca tgggttatgg
      781 agatgaacag tataacggat ttcagagaaa gactgtgtga gcttgaagct gcgcttccgt
      841 cagtttcctg gatgatatct tcttcctcgc ttactgaact agctgctcat ttcagaagga
      901 atatgaatac agaactaccg gatggacgga ttgcgttgct gcgtttccat gatccgcgcg
      961 ttcagaagcg cctgggagaa atgctgaacg accaacaaca tcgggagctg acggggttaa
     1021 tgcaggagtg gctgacaata gtggacggaa aagcctggtc atttaaacaa agggagttca
     1081 tatgctgaag ttgtctgaag agcagtgtgt tggtatggac aaaagggaag atgatgattt
     1141 tgttcgcgac accgcgctaa gactgaagaa gaaatttccg ggaattactg aatcagatga
     1201 cacgttccat gttcgcctga gaaaagcgct ggattatgcc aatacccttc cgcttaaaga
     1261 gaaaaacgtg aagcgggact tcttgtttct ggaggccttc tggcctggtt tttatctgaa
     1321 acccgaagtg gataaatggt tgagaacgcc gaatgcttat tcaactgacc agcgggttga
     1381 agattataag catgtgatga taaacagaga aaggagggag ctctgatggt tatggtcctg
     1441 acaggggctg gtatggggag cgcagctacc ggaggtgttg gtatggctgg tgcctcaacc
     1501 ggaacagcag tatttcccgg agcaggaagc cgcgatggag actgggaaga agataatata
     1561 tcttatcctg gctcaggcag tggttcgcgt cagggtaacg gagggtttta ttatcagggg
     1621 aaacagtatg atccaattct gaatgagccg atggttgtca gcgcagagac gcatggtgat
     1681 ttctgggaag atgatatgga tcattcagaa acagtaaact atgaaggtat tgatagcaaa
     1741 gccgaagtac aggcgaagac ggatgagaaa acgtgtgaga actgcccacc ggaagggaag
     1801 gtaatgccga tgataaggcg ttgctcgcgc tggagtatgg taacaatcag ctaccagaca
     1861 agaatatgtg gcacgtttta taatccggag acgcaacaga tacaggaatt taagtactgt
     1921 ggcgtatcgt ttgatggctg gaaagataag ctgtgtcagt tctgggaagc taaagcgcgg
     1981 tatgaccaat ttttcagggc tgatggttca aagaaaccct ggtggacagg taatctaagc
     2041 gcaattaatc aagcatcccg acatcaggcg gttgcaacag tcaatcagcc attgaaagtt
     2101 gtctggatat ttatgcaacc tgtaagttgt gagtatttca ggaaaatgtt taaagattac
     2161 aaggatatta ttacgaggtg gcaaccgtga ttgaattatt taacacgtca ataaatatac
     2221 attctttgaa agaaagagac ataacgatag acgattgttt gaatgaacta tacagaagta
     2281 ccgtagttgt taaaaatcta acgcagcacg atcacatatg gtacttaact ggctactcaa
     2341 gaaaggacgc ttcaaagcat gtcgtgttta gtgataatga accgaaccaa aatacaatag
     2401 atacctttga gcggcattat aaaaagaatc gtcctttaat taatgaatct atatggaatg
     2461 gacaacc
//
LOCUS       DAZUCC010000378         2454 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZUCC010000378 DAZUCC010000000
VERSION     DAZUCC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.380"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            218..1882
                     /gene="asnB"
                     /locus_tag="ACHY09_005066"
     CDS             218..1882
                     /gene="asnB"
                     /locus_tag="ACHY09_005066"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFS3887885.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2263..2339
                     /locus_tag="ACHY09_005067"
     tRNA            2263..2339
                     /locus_tag="ACHY09_005067"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2297..2299,aa:Met,seq:cat)
     gene            2348..2432
                     /locus_tag="ACHY09_005068"
     tRNA            2348..2432
                     /locus_tag="ACHY09_005068"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2382..2384,aa:Leu,seq:tag)
BASE COUNT          637 a          605 c          617 g          595 t
ORIGIN      
        1 cccatcatta aaatcacccc attcaataaa acccgtcaaa aattatcgat ccatcaataa
       61 atatacccaa aagccctccc ctttttcatc aatcagcaat cgccattacg ttttttattt
      121 ttccactgcc aaatcgcttg cgcaccgtgc tgctttgcgg cattttttta acaagtaaac
      181 acaacacgca gtaaacacca ggttaacgga gaaggttatg tgttcaattt ttggcgtatt
      241 cgatatcaaa acagacgcag ttgagctgcg taagaaagca ctcgagctgt cacgcctgat
      301 gcgtcatcgt ggcccggact ggtccggtat ttatgccagc gataacgcca tcctcgccca
      361 cgaacgtctg tcaattgttg acgttaacgc gggggcgcaa cctctctaca accaacaaaa
      421 aactcatgtg ctggcagtaa acggtgaaat ctacaaccac caggcattgc gcgctgaata
      481 tggcgatcgt taccagttcc agaccgggtc tgactgtgaa gtgatcctcg cgctgtatca
      541 ggaaaaaggg ccggaatttc tcgacgactt gcagggcatg tttgcctttg ccctgtacga
      601 cagcgaaaaa gatgcatatc tgattggtcg cgaccatctg gggatcatcc cactgtatat
      661 gggctatgac gaacacggtc agctgtatgt ggcctcagaa atgaaagccc tcgtgccagt
      721 ttgccgcacg attaaagagt ttccggcggg gagctatttg tggagtcagg acggcgaaat
      781 ccgttcttat tatcatcgcg actggttcga ctacgatgcg gtgaaagata acgtgaccga
      841 caaaaacgag ctgcgtcagg cactggaaga ttccgttaaa agccatctga tgtctgatgt
      901 gccttacggt gtgctgcttt ctggtggtct ggattcctca attatttccg ctatcaccaa
      961 gaaatacgcc gcccgtcgcg tggaagatca ggaacgctct gaagcctggt ggccgcagtt
     1021 gcactccttt gctgtaggtc tgccgggctc accggatctg aaagcagccc aggaagtggc
     1081 aaaccatctg ggcacggtgc atcacgaaat tcacttcact gtacaggaag gtctggatgc
     1141 catccgcgac gtgatttacc acatcgaaac ttatgatgtg accactattc gcgcttcaac
     1201 accgatgtat ttaatgtcgc gtaagatcaa ggcgatgggc attaaaatgg tgctgtccgg
     1261 tgaaggttct gacgaagtgt ttggcggtta tctatacttc cacaaagcgc cgaacgctaa
     1321 agagttgcat gaagagacgg tgcgtaaact gctggccctg catatgtatg actgcgcgcg
     1381 tgccaacaaa gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa
     1441 attccttgat gtggcgatgc gcattaaccc acaggataaa atgtgcggta acggcaaaat
     1501 ggaaaaacac atcctgcgtg aatgttttga agcgtatctg cctgcaagcg tggcctggcg
     1561 gcaaaaagag cagttctccg atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt
     1621 ggcagcgcag caggtgtctg atcaacagct ggaaactgcc cgcttccgct tcccgtacaa
     1681 cacgccgacc tcaaaagaag cgtatctgta ccgggagatc tttgaagaat tgttcccgct
     1741 tccgagcgcc gctgagtgcg tgccgggcgg tccttccgtc gcttgttctt ccgctaaagc
     1801 gattgagtgg gatgaagcgt tcaagaaaat ggacgatccg tctggtcgcg cggttggcgt
     1861 tcaccagtcg gcatataaat aagacgtgta atattgcccc ggagaaatcc ggggcaattt
     1921 cagttactgg ggcgcttgtt gattctgata tgcagtaagg aaatgttccg ggtttgccac
     1981 atcgctgttt gacaactcat tactgtaggc cagtatttca tagattaatt tcccaccact
     2041 attgttgaat tccgaatttc atcctgtatg acaaaagaat ggctgtaggg tggggaaaaa
     2101 acacaaagta aacaataatt gacgaatata gcgccatgct gttcgcaacc taaccaaaca
     2161 gtcactttcg agcaattttc cttgaaaaag aggttgacgc tgcaaggctc tatacgcata
     2221 atgcgccccg caacgccgat aaggtatcgc gaaaaaaaag atggctacgt agctcagttg
     2281 gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccat
     2341 cttttttgcg ggagtggcga aattggtaga cgcaccagat ttaggttctg gcgccgcaag
     2401 gtgtgcgagt tcaagtctcg cctcccgcac cattcaccag aaagcgttgt acgg
//
LOCUS       DAZUCC010000379         2450 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZUCC010000379 DAZUCC010000000
VERSION     DAZUCC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.381"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            50..2317
                     /gene="adiA"
                     /locus_tag="ACHY09_005069"
     CDS             50..2317
                     /gene="adiA"
                     /locus_tag="ACHY09_005069"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFS3887886.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          616 a          673 c          609 g          552 t
ORIGIN      
        1 gaagatactt gcccgcaacg aagattcctt cataaccggg taagcaatga tgaaagtatt
       61 aattgttgaa agcgagtttc tccatcaaga cacctgggtc ggtaacgccg ttgagcgtct
      121 ggcagatgct ttaagccagc aaaatgttac cgtgattaaa tccacctcct ttgatgatgg
      181 ttttgccatt ctctcttcaa acgaagccat tgactgcctg atgttcagct atcaaatgga
      241 acatccggac gaacatcaaa acgtcagaca attgatcggt aagcttcatg agcgtcaaca
      301 aaacgtgccg gtcttcctgt tgggcgatcg ggaaaaagcc ctcgccgcaa tggaccggga
      361 tctgctggag cttgtcgatg aattcgcctg gattctggaa gataccgccg actttatcgc
      421 cggacgcgcc gttgccgcga tgacccgcta ccgccagcaa ctgttgccgc cactgttcag
      481 cgcgctgatg aaatatagcg acatccatga atattcctgg gcagcgccag gccaccaggg
      541 cggcgttggt tttaccaaaa cacccgccgg acgtttctac catgactact atggtgaaaa
      601 tctgttccgc accgacatgg gcatcgaacg aacttccctc ggttctttgc ttgaccatac
      661 tggcgcattt ggcgaaagcg aaaaatatgc tgcacgcgta tttggtgccg atcgctcctg
      721 gtcggtagtc gtcggtactt ccggctctaa ccgcaccatc atgcaggctt gcatgaccga
      781 taacgatgtc gtggtcgttg accgtaactg ccataaatcc atcgaacaag gcttgatgct
      841 gacaggcgcg aaaccggtct acatggtgcc aagccgcaac cgctacggca ttatcgggcc
      901 aatctatccg caggaaatgc aacctgaaac cttgcagaag aaaatcagtg aaagcccgct
      961 gaccaaagac aaagccgggc aaaaaccgtc ttactgcgtg gtgaccaact gtacctatga
     1021 cggcgtgtgt tataacgcta aagaagcgca ggatctgctg gaaaaaacct ccgatcgtct
     1081 gcactttgac gaagcctggt acggctatgc acgtttcaac ccaatctatg ccgatcacta
     1141 tgccatgcgc ggcgaacctg gcgatcacaa cggccctacc gttttcgcca cccactccac
     1201 ccacaaactg ctgaatgcgc tgtcacaggc ctcttatatt catgtacgtg aaggtcgtgg
     1261 ggcgattaac ttctcccgct tcaaccaggc ctacatgatg catgccacca cctccccgct
     1321 gtatgccatc tgcgcatcca acgacgtggc ggtgtcgatg atggacggca acagcggcct
     1381 gtcactgacg caggaagtga tcgacgaagc ggttgatttc cgtcaggcga tggcgcggct
     1441 gtataaagag ttcaccgctg acggtagctg gttcttcaaa ccgtggaaca aagaagtcgt
     1501 caccgaccca caaaccggca aaacctatga ctttgctgac gcaccaacca aactgctgac
     1561 caccgttcag gactgctggg tcatgcatcc gggcgaaagc tggcacggct tcaaagatat
     1621 tccggataac tggagtatgc tcgacccgat taaagtcagc atccttgctc cgggaatggg
     1681 tgaagatggt gaactggaag aaaccggtgt tccggcggcg ctggtcacag cctggcttgg
     1741 tcgccacggc attgtgccta cccgtaccac tgacttccag attatgttcc tgttctctat
     1801 gggcgtgacc cgtgggaaat gggggactct ggttaacacc ctttgctcct tcaaacgcca
     1861 ctatgacgcc aacaccccgc tggcgcaggt gatgccggaa cttgttgaac aatatcctga
     1921 cacttacgcg aacatgggga ttcacgatct gggtgacacc atgtttgcct ggctgaaaga
     1981 aaacaaccct ggcgcacggt tgaacgaagc ctattccggc ctgccggtgg cggaaatcac
     2041 cccgcgagaa gcgtacaacg cgattgtcga caacaatgtc gaactggtat ccattgaaaa
     2101 cctaccagga cgcatcgcgg caaactcagt tatcccgtat ccgccaggaa tcccgatgct
     2161 gctgtctggt gaaaacttcg gcgataaaaa cagtccgcaa gtaagttatt tacgctcgct
     2221 gcaatcctgg gaccatcatt tccctggatt tgaacacgaa actgaaggga ctgaaattat
     2281 tgacggtatt taccacgtta tgtgtgtgaa agcgtaacca ctattccgct gaaggcgtaa
     2341 ttgttaaata acattacgcc gcctggcttg aggcctttta agtatggcaa cgttttcata
     2401 aaaattgctg caaacaaaaa atgtcatgct ttttgcgcgg tcccaccccg
//
LOCUS       DAZUCC010000380         2444 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZUCC010000380 DAZUCC010000000
VERSION     DAZUCC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.382"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..384
                     /gene="repA"
                     /locus_tag="ACHY09_005070"
     CDS             121..384
                     /gene="repA"
                     /locus_tag="ACHY09_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFS3887887.1"
                     /translation="MSDAVRSATSSEARPKRAYRKGAPLSVNERKRLSLNRKRDTHKA
                     LNVFIRNELKDILERFCQDKHLTLGEGIELLIQQEKERAKNGE"
     gene            607..681
                     /gene="tap"
                     /locus_tag="ACHY09_005071"
     CDS             607..681
                     /gene="tap"
                     /locus_tag="ACHY09_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFS3887888.1"
                     /translation="MPGKVQYFFLCSLLLRIVSAGWCD"
     gene            674..1531
                     /gene="repA"
                     /locus_tag="ACHY09_005072"
     CDS             674..1531
                     /gene="repA"
                     /locus_tag="ACHY09_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFS3887889.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALFSSLDI
                     SEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
BASE COUNT          619 a          641 c          629 g          555 t
ORIGIN      
        1 tagctgaact ggcgatctgt gcgccaacat ggatccgcca gggtataatt aagtcgctga
       61 tttcaataaa gtgtagtatc ctctgcaaaa tgatcctttt ttgatctatg aggaggctgt
      121 atgtctgacg ctgtacgttc ggcaacttcc tctgaagcca gacctaaacg ggcatataga
      181 aaaggtgcac cgttaagcgt taatgagcga aagaggttgt ctctcaatcg aaagcgggat
      241 acacataaag cgcttaatgt tttcatccgt aatgaattga aagatattct tgagcgcttt
      301 tgccaggata agcatttgac cttgggtgaa gggatcgagc tgctaatcca gcaggagaag
      361 gaacgagcta aaaatggtga gtagtcacct ttatttgatc cttttctggc agagtgatag
      421 attacggaca gaaaattcat ggctggccac gccgtaaggt ggcagggaac tggttctgat
      481 gtggatttac aggagccaga aaagcaaaaa ccccgataac ctttctatgt ttggcgacgc
      541 agaagattac cgggggccta caaaaccgta tagccaacaa ttcagctatg cggggagtat
      601 agttatatgc ccggaaaagt tcaatacttc tttctgtgct cactccttct gcgcattgta
      661 agtgcaggat ggtgtgactg atcttcaaca aacgtattac cgccaggtaa agaacccgaa
      721 tccggtgttc acaccccgtg aaggtgcagg aacgctgaag ttctgcgaaa aactgatgga
      781 aaaggcggtg ggcttcacct cccgttttga tttcgccatt catgtggcgc atgcccgttc
      841 gcgtggtctg cgtcgacgca tgccaccggt gctgcgtcga cgggctattg atgcgctcct
      901 gcaggggctg tgtttccact atgacccgct ggccaaccgc gtgcagtgct ccatcaccac
      961 gctggccatt gagtgcggac tggcgacgga gtctgaggcc gggaaactct ccatcacccg
     1021 tgccacccgt gccctgaagt tcctgtctga gctggggctg ataacctacc agacggaata
     1081 cgacccgaca attggctgta acatcccgac cgacatcacg ttcacgccgg cactgttttc
     1141 ctcactcgat atatcagaag aggcggtggc ttccgcacgc cgcagccgcg tggaatggga
     1201 aaacaagctg cgcaaaaagc aggggctgga tgcgctgggt atggatgaac tgatagcgag
     1261 agcctggcgt tttgtgcgcg agcgtttccg tagttatcag gcagagctta agtcccacgg
     1321 catgaaacgt gcccgtgcgc gccgcgatgc aggcaggacc cgtcaggaca ttgtcaccct
     1381 ggtgaaacgg cagctgacac gtgaaatcgc ggaagggcgt ttccggggaa gcctggaggc
     1441 cgtgaagcgc gaaatcgacc gtcgcgtgaa ggaacgcatg ataatgtcgc ggaacaacaa
     1501 ctacacacgg ctggccacag cctccccctg agttttacct catctgaata atccggcctg
     1561 cgccggaggc ttccgcacgt ctgaagaaaa aagcagctca gcacataaaa aacagcctca
     1621 tcatctgctt tccggctcgc aatgctcccc cggtttttca cgcaaaacac gtctcacaga
     1681 cggagaattc cgcttatcca catttaactg caagggaatt ctccataagg ttacaatcgt
     1741 tcatgtcata aagtgccagc cgcaggcgtt acaggctgca atgtttcttt taaaccccag
     1801 tactgtattt cctttaaact acttaattac ttccatttaa aaagaaaacc tattcactgc
     1861 ctgtcctgta gacagacaga tatgcacctc ccaccgcaag cggcgggccc cgaccggagc
     1921 cactttagtt acaacactca aatacaagca ccggaaaaaa cccggtcccg cgcagaactg
     1981 aaaccacaaa gcccttccct cataactgaa aagcgccgcc gcccccgccc gaagggcggg
     2041 aacaacatcg cttttaatca tgaatgttgt aactacaatc catcatcgct gtcagtcttc
     2101 tggctggatg ttctcagtac acgctcgtaa gcggccctga cggcccgcta acgcggagat
     2161 acgccccgac ttcgggtaaa ccctcgtcgg gaccactccg accgcgcaca taagctctgt
     2221 catggctgac agcgggtata gctttgcagg gtggggatgg gtaaggtgaa atctattaat
     2281 cagtaccggc ttacgccggg cttcggcggt tttactccgg tatcatatga aacaacagag
     2341 tgccgccttc catgccgctg atgcggcata tcctggtgac gatatctgaa tcgttatata
     2401 ctgtgtatat acgtagtaat gacgaggtga taaatggcac aggt
//
LOCUS       DAZUCC010000381         2431 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZUCC010000381 DAZUCC010000000
VERSION     DAZUCC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.383"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..1227
                     /locus_tag="ACHY09_005073"
     CDS             88..1227
                     /locus_tag="ACHY09_005073"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS3887890.1"
                     /translation="MSTMKPAIFSFFSGSGFLDLGFENAGYEVVFVNEFHPPFMDAYK
                     HSRKKLGKQPPRFGYAQESIETIEHSVIKHSMKTLKGENRLVGFIGGPPCPDFSVAGK
                     NKGKDGENGKLSQSYIDLIIEMMPDFFLFENVKGLYRTLKHREFFEQIKNELASVGYI
                     LHERLINSIEYGAPQDRERIILVGFLDKSLAKNFEWSGQIYPNKEAFQFPWPTTNTYE
                     EDSSMECPQGIPEELTIEYWFEKNDVTNHPNAKHVFTARAGLARFQSVEEGDDSKKSY
                     KRLHRWRYSPTAAYGNNEVHLHPYKPRRITVAEALAIQSLPKNFELPGDMTLSNMFKT
                     VGNGVPYIAALGIAQSILQHLGYSNEDNSIESGSGDFELTQKYAV"
     gene            1163..2236
                     /locus_tag="ACHY09_005074"
     CDS             1163..2236
                     /locus_tag="ACHY09_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS3887891.1"
                     /translation="MRITASNLVQAISSLPKNTLFNYINDRNSGKIEIVRIQHPEGPI
                     EIKRFDPKKGQTQATAKTESISTQMLWRLANALEENRPVNVERVFGASYNTRSVLESL
                     LAHTPEFYWCRPARLEIMNAQKSIKPGHKHLIFLPDMPHTNGLLIEHQTNIVVSEMSF
                     DVVHQSVDIETIKPTKGMTIEEKRRHAQIQIALVKIGYCLGLDTWVAANDRSLQYNGK
                     AIAQMDGVISSLSDEQVLQSYNDAIKDARLIDCIWFREGRMMPAVMEIEHSTGIKSGL
                     TRMKQFYDHAPRLQDIRWTIVAPDEYRAKVIEFANMPQFIELKAKFFPYSSVEELYSL
                     CDRRKPKGINDLFLDSFMEDCVI"
BASE COUNT          814 a          457 c          473 g          687 t
ORIGIN      
        1 ttgataatta acaagttatc atgtaaaata gtaggatcgt acagcgagat atttagtaaa
       61 aatttaagct aacttgatag gtataaaatg tccacaatga aacccgctat attttcattc
      121 ttttcaggat caggttttct tgacctaggc ttcgaaaatg ccgggtatga ggtggttttc
      181 gtcaatgaat tccatcctcc cttcatggac gcgtataaac actcaagaaa aaaacttggc
      241 aaacagccgc cacgctttgg gtatgcccaa gaaagcattg aaactattga gcattcagtg
      301 attaagcata gcatgaaaac actgaaaggt gaaaaccgcc tagtgggctt cattggcgga
      361 ccaccatgcc ctgacttttc tgttgctggc aaaaacaaag gcaaagatgg tgaaaatggc
      421 aaattatcac aaagttacat tgacctgata attgaaatga tgcctgattt cttccttttt
      481 gaaaacgtta aaggcttata ccggactctt aaacatagag aatttttcga acaaattaag
      541 aatgaactag cttcagtagg atatatcctg catgaaagat tgattaattc aattgaatac
      601 ggtgcgcctc aagatcgaga aaggattatt ttagttggtt ttcttgataa gtctttagcc
      661 aaaaacttcg aatggagtgg ccaaatatac cccaataagg aagctttcca gttcccttgg
      721 ccaacaacaa acacatacga agaagactca agtatggaat gcccgcaagg tattccagaa
      781 gaacttacta tagaatactg gttcgagaaa aacgatgtta ccaatcatcc aaacgctaaa
      841 catgtattta cggcccgagc cggtttagca agattccagt cagtagaaga aggcgacgat
      901 agtaaaaaat catataaaag gcttcatcgc tggagatact ctccaacagc agcatatggt
      961 aataatgaag ttcaccttca cccatacaaa cctcgtcgta ttacggtcgc tgaagcattg
     1021 gcaatccagt ctttgccaaa aaattttgag ctaccagggg atatgacttt gtcgaatatg
     1081 tttaaaacag tcggaaacgg agttccctat atcgccgcgc tcggtattgc ccaatcaatt
     1141 ttgcaacatt taggatacag taatgaggat aacagcatcg aatctggttc aggcgatttc
     1201 gagcttaccc aaaaatacgc tgtttaacta tataaacgat aggaatagcg ggaagattga
     1261 gattgtacgt atccaacatc ccgaaggtcc tatcgaaata aagagatttg atcctaagaa
     1321 aggtcaaact caagcaacag ctaaaacaga gagtatttcg actcaaatgc tttggcgact
     1381 tgcaaatgca ctcgaagaaa acagacctgt caacgttgaa agggttttcg gtgccagtta
     1441 caacacccgc tcggtattgg agagcttgct cgcacatact ccagagttct attggtgtag
     1501 gccagccaga ctggaaatca tgaatgcaca aaagtctata aaaccaggac ataagcatct
     1561 gatttttctg ccagatatgc cacatactaa tggtttgtta attgagcacc agactaatat
     1621 tgttgtctct gaaatgagct tcgatgttgt tcaccaaagc gttgatattg agacaattaa
     1681 accgactaaa ggcatgacta ttgaagaaaa aagacgtcat gctcagatac aaattgcctt
     1741 agtaaaaata ggttattgtt taggtttaga tacttgggta gcagccaatg atcgtagctt
     1801 acaatataat ggtaaagcta tagcgcaaat ggacggtgta ataagttcat tatctgatga
     1861 gcaagtactc caatcgtata atgatgcgat taaagatgct cgtcttatag actgcatttg
     1921 gtttagggaa ggccgcatga tgccagcagt aatggagatt gaacacagta ctggtataaa
     1981 aagtggtctg actcgcatga aacaattcta tgatcatgca ccgagattac aagatatccg
     2041 ttggactatc gttgctcctg atgaatatcg agcaaaggtg atagaatttg caaacatgcc
     2101 ccaattcatt gaattaaaag ctaaattctt cccttactct tcagttgaag aattatactc
     2161 tctatgcgac cgaagaaaac ctaaaggaat aaatgattta tttctagatt cttttatgga
     2221 agattgtgtt atatagctaa gaacatcaaa ttaacatgaa cctactgata tatactatca
     2281 gtaggtttaa aatgatttca aatgtgttcc ccaatatgag ctattgccac tctgttttta
     2341 tcacttatca taataaatcc aaaataaatt ctattcttat ggtgttttct gtttccctta
     2401 gtatcatcaa cattttctaa tttcaaatgg a
//
LOCUS       DAZUCC010000382         2427 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZUCC010000382 DAZUCC010000000
VERSION     DAZUCC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.384"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1399
                     /locus_tag="ACHY09_005075"
     CDS             <1..1399
                     /locus_tag="ACHY09_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFS3887892.1"
                     /translation="TGKTDALLSVIMRKRGNIYQSDGERAPNKEKPAALFAKGSVSIT
                     DSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDVQFWYQYSPADNSLINVK
                     STLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFPGAHQLSYGAEYYRQQQK
                     PEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDRYKSFNPRAGKLKAERLS
                     PRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRKGKPNYWVPNLNLKPENN
                     ITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVDMKRMRSYSYNVSRARIW
                     GWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTLISDISIPVGHGGVSAGW
                     RAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEASVTLDNAFNKLAMNGKGV
                     PLSGRTVSLYTRYQW"
     gene            1418..2209
                     /locus_tag="ACHY09_005076"
     CDS             1418..2209
                     /locus_tag="ACHY09_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFS3887893.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT          732 a          561 c          543 g          591 t
ORIGIN      
        1 aaccggaaaa acagatgcgc ttctttctgt cattatgcgt aaaagaggaa atatctatca
       61 gagtgatggt gagcgtgcac ctaacaagga aaaacctgca gccctgtttg cgaaaggctc
      121 tgtcagtata acagacagta acaaagcagg tgccagcttg cgtctctacc agaataacac
      181 cactgaaccg ggcaatccca ctctgacaca tggtgacaac ggcctgcgtg acagaaaaac
      241 agcacaaaat gacgtgcagt tctggtacca gtactcccct gcggataaca gcctcatcaa
      301 tgtaaagtca acgttatatc tcagtgatat cactatcaag acaaacggtc acaacaaaac
      361 agcagaatgg cgaaacaaca gaacctccgg tgttaatgtt gtcaacagga gccatactct
      421 gatttttccg ggagcccatc agttaagtta tggcgccgaa tattaccgtc agcagcagaa
      481 gccggaaggc tctgccacac tatatccgga aggaaacatt gactttacat cgttgtattt
      541 ccaggatgaa atgacaatga aaagcaaccc ggttaacatt atcgtcggtt cccgttatga
      601 ccggtacaag agcttcaatc cccgagccgg aaaactgaaa gccgaacgcc tgtcccccag
      661 ggcggcgatt tcagtctcac caacagactg gctgatgatg tacggctcca tatcctctgc
      721 attccgtgcc cccacaatgg cagaaatgta cagggatgat gtacattttt accgcaaggg
      781 taaacccaat tactgggtac ctaaccttaa tctgaaacca gaaaataaca ttacccgtga
      841 gattggcgca ggtattcaac tggacggcct gcttacagac aatgaccggc tgcagttgaa
      901 aggcggatat ttcggaacgg atgccagaaa ctatattgcc acgcgcgtgg atatgaaacg
      961 gatgcgttct tattcttata atgtatcccg ggcccgtatc tggggatggg atatgcaggg
     1021 taattaccag tctgattatt ttgactggat gctttcttat aaccgcacgg aaagtatgga
     1081 tgccagtagc agggaatggc tgggctccgg caatcctgac acactcatca gtgacatcag
     1141 tatacctgtt ggtcatggag gagtttctgc cggatggcgt gctgaacttt cggcagcagc
     1201 cacgcatgtg aaaaaaggcg atccccatca ggctggctat gccgtacatt ccttttcact
     1261 gtcttataag cctgtaagtg ttaaaagctt tgaggcatca gtgactctgg ataatgcctt
     1321 caacaagctt gccatgaatg gcaaaggtgt gccgctttca ggcagaactg tcagtcttta
     1381 tacccgttat cagtggtaac ctcgcaggaa ataaatcatg atgaaaaata caggctatat
     1441 cttagctctt tgtctgacag catcggagca tgccctggcc cacgatgtct ggattaccgg
     1501 taaacaggca gagaacaacg ttaccgcaga gattggttat ggtcataatt tcccctcaaa
     1561 gggcacaatt cctgacagaa ggaatttctt tgaaaatccc cggctttata acgggaaaga
     1621 gataataacg ctgaagccaa cgtccacgga ttatgtctat aaaactgagt ctgcagccaa
     1681 cggtaatagc tacgttctgt caacgaaaat gaaaccggga tactggtcga gaacctcgtc
     1741 aggatggaag cctgtcagtc gggagggcag aaatgatgtg gcatactgtg agtttgtgac
     1801 taaatatgca aaatctttta ttcctggtga acagcagatg ccagcacaac tctatcagtc
     1861 tccaacaggg gatgagcttg aaatcattcc attatccgat atcagtcgtt tcggtgaaga
     1921 tgtgaagctg aaagttctgt ataaaacatc cccgcttgcc ggagctactc tggagcttga
     1981 ctctgtcagt tatctgaaat catcccgtca tacccatgca gctgagcata aacattcggc
     2041 tcataaagca gaacttacct ttgtaagtaa tgaggacggt atcatcactg taccttccct
     2101 tcatgtcggg caatggctgg ctaaagtcaa aaataagaaa gtatttccgg acaagagtct
     2161 gtgtgatgaa actgtcgatg tggcaacctt aagcttctcc cgaaattaat aatactattc
     2221 ctgccctgat ttatgggcag ggtattatca atacgagaat agcctaccga catccgtgta
     2281 aaaaaccaca taatgatttt tcatatccat ttccagatat acttatgtaa agatcacctc
     2341 caaaaaagcg taatagtaat aaaaatccct gaagcaccct gttcaaacca gaaggaatac
     2401 ataacaagac aataaaatgt gaaatcc
//
LOCUS       DAZUCC010000383         2396 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZUCC010000383 DAZUCC010000000
VERSION     DAZUCC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.385"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            18..701
                     /locus_tag="ACHY09_005077"
     CDS             18..701
                     /locus_tag="ACHY09_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFS3887894.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSA
                     ETRQRKNKK"
     gene            745..>2396
                     /locus_tag="ACHY09_005078"
     CDS             745..>2396
                     /locus_tag="ACHY09_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFS3887895.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISVLVASG
                     LLSSFGALANNYDGQPTDYGDGSAGDGWVAIGKGAKANTFMNTSGASTALGYDAIAEG
                     EYSSAIGSKTLATGGASMAFGVSARAMGDRSVALGASSVANGDRSMAFGRYAKTNGYT
                     SLAIGDSSLADGEKTIALGNTAKAYEIMSIALGDHANASKEYSMALGASSAASAANAI
                     AVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIALG
                     SGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALGQG
                     SYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDIST
                     TSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGLDN
                     NTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNTAN
                     ITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKIT"
BASE COUNT          695 a          525 c          614 g          562 t
ORIGIN      
        1 gaaaaggaat tcatcctatg aacctaaaga aaacactgtt aagcgtgtta ataatagtgc
       61 ctctctgctt attggcaggg tgtgactata ttgaaaaagc gagtaaggtc gacgacctcg
      121 ttacacagca agagttgcaa aaaagcaaaa ttgaggcgct tgagaaacaa caggaactcg
      181 ataagcgaaa gatagaacat tttgaaaaac aacaaactac tatcataaat agtaccaaga
      241 cgctcgctgg cgtggtgaag gcggttaaag acaaacagga cgaatttgtc tttacagaat
      301 ttaacccggc acaaacccaa tacttcattt taaataacgg ctctgttggt ttggcaggcc
      361 gggtgctggc tattgatgca gttgaaaatg gcagcgttat tcgtatttcg ctggttaatt
      421 tattaagcgt tcccgtatca aatattggct ttcatgcgac atggggcaat gaaaggccca
      481 ccgatgccaa agctctggca aagtggcaac aattactttt caacaccaca ctgaattcca
      541 ctctgcaatt aatgcctggt caatggcaag acattaatct gacattaaaa ggcgtttcgc
      601 ctaataacct gaagtatctg aagttgtcca tcaatatggc caatctccag ttcgacactg
      661 ttcaatctgc tgaaactcgg cagcgaaaaa acaaaaaata aatgcagctt ctgcgcttat
      721 atcaaggaat agagagcatc aataatgaac aaaattttta aagttatctg gaatccagcg
      781 acgggcagtt acagcgttgc cagcgaaacg gcgaagagcc gtggtaaaaa aagcgggcgc
      841 agtaagctgt taatttctgt actagttgcg agtgggttgt tgtcgtcgtt tggagcgtta
      901 gctaataact acgacgggca gccaactgat tatggcgatg gctcagcagg tgacggctgg
      961 gttgctatcg gtaaaggggc aaaagcaaat acctttatga acactagtgg cgcgagtaca
     1021 gctttaggat atgacgcgat agccgaaggt gagtacagtt ctgccatcgg gtcaaaaacc
     1081 cttgcaactg gtggagcatc catggcgttc ggggttagtg caagagcaat gggtgacaga
     1141 agtgtcgcgc tgggtgcatc gtcagtagca aatggcgatc gttcgatggc ttttggtcgt
     1201 tacgcaaaga cgaatggtta tacatctctt gctattgggg actcctccct tgccgatggt
     1261 gaaaaaacta ttgcgttagg aaatacggct aaagcttacg aaattatgag catcgccctc
     1321 ggtgatcatg ccaatgcgtc aaaagaatat tcaatggcgc tgggtgcaag tagtgccgca
     1381 tctgcggcta acgcgattgc agtaggccgc aacagtgctg cagctggcgt agacagcctc
     1441 gcattcggta gactatccgc cgccaacgcc gcaaacgcga ttgctatggg tgcagagagc
     1501 aaagctgctg aaaatgcgac ggcagttggt actaacgcgg aggcaaatgg acttaatagt
     1561 atcgcgttgg gctccggcag tattgcagat gtcgacaaca ctattgctct gggcaatcaa
     1621 agtcaggcag tagcggctgg cgcaattgcc atcggtcaag ggaataaagc tgacggcgca
     1681 aatgctatcg cgctgggtaa tggtagtatt acaggtggtg taaatgctat tgctcttgga
     1741 caaggcagtt atgccggttt agaaaatggc actgcaattg gtgctcaagc cagtgctcag
     1801 gggaaaaatt cagttgctct gggtgctggt tctgtagcga ctgacgcgga tactgtttct
     1861 gtgggtaaca caacagctca gcgacaaatt gtcaatatgg cagcaggtga tatcagcact
     1921 accagtactg atgccatcaa tggatcacag ctttatgcta tcagtaagtc agtagcggac
     1981 aatcttggtg ggggggctac cgtcaatgcg caaggcgtcg ttacttcccc aaattacagg
     2041 ctgaaaagtg gtattttcgg cactgttggc gacgccttaa cgggcctgga caataatacg
     2101 ttacaatggg actcccttaa aaaggcatat agtgcggcac atggtacaga tactaccagt
     2161 accatcacca acgttaaaga cggcgctatt tctgatacca gtaaggatgc ggttaacggt
     2221 tctcagctga aaaccaccaa cgataacgta gcgaccaata ctgccaatat caccaccaac
     2281 actaacagta tcaataccct gacggattcc gttggcgacc ttaaagacga tgccctgctg
     2341 tggaatggca ccgcgttcag cgccgcgcac ggcaccgaag ccaccagcaa aatcac
//
LOCUS       DAZUCC010000384         2394 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZUCC010000384 DAZUCC010000000
VERSION     DAZUCC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.386"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            28..537
                     /gene="eco"
                     /locus_tag="ACHY09_005079"
     CDS             28..537
                     /gene="eco"
                     /locus_tag="ACHY09_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFS3887896.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(687..2333)
                     /gene="mqo"
                     /locus_tag="ACHY09_005080"
     CDS             complement(687..2333)
                     /gene="mqo"
                     /locus_tag="ACHY09_005080"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFS3887897.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          560 a          669 c          601 g          564 t
ORIGIN      
        1 agcgacatca tcggtgaaaa cctataaatg aagaaggaaa gcaaaaaaat gaagaccatt
       61 ctacctgcag tattgtttgc cgctttcgcc accacttccg cctgggcggc agaaagcgtc
      121 cagccactgg aaaaaatcgc accttatcca caagctgaaa aaggcatgaa gcgccaggtg
      181 attcagttaa ccccgcaaga agatgaatct accctgaaag tagaactgtt aatcggccag
      241 acgctggaag tcgattgcaa tttgcatcgt ctcggcggga agctggaaag caaaacgctg
      301 gaaggctggg gctatgatta ttatgtcttt gataaagtca gttccccggt ttcgacgatg
      361 atggcctgcc cggatggcaa gaaagagaag aaatttgtca ccgcgtatct gggcgatgcc
      421 ggaatgctgc gttataacag caagctgccg atcgtggtgt atacgccaga caatgtagat
      481 gtgaagtacc gcgtctggaa ggcggaagag aaaattgaca acgcggtagt tcgctaaact
      541 gctgtgaagt gctgcaaccg ccaggtcgga taaggcggtt acgccgcatc cgacatctaa
      601 cgcccgagcc ggttgcctga tgcgacgctg acgcgtctta tctgcatacg ccacatccgg
      661 cataccatgc cggatgtggc acatcatcac aacgcaatat ccgccacttc tttttgaacg
      721 ggttgcggtt tcaactgcgg tttcggcgta ctatctgctg cctgcggttt gtcgtatttc
      781 agccccagca cttcgctggt gtactgcaac tcgcgttctg ttgccgctac atcaccgttc
      841 agcttgcgtc cataagacgg aacgatcgct ttcaacgtag cctgccattg cgggctggaa
      901 acgcgatcgc caaatacttt ttccagcaga tccaacataa tcggcgcggc ggttgacgcc
      961 cctggcgatg cccccagcag cgcggcaatg gttccttgtt ggtcgctgac cacttcggta
     1021 cccagacgca gtacgccacc tttctcggca tcacgcttga taatctgcac gcgctgcccc
     1081 gcttgccaca aacgccagtc ctcttttttc gcctgcggat agtactcttt caacgcttca
     1141 aaacgatcct cttcactcaa catcacctga ctcaccagat atttcaccag atcgaaatta
     1201 tccagcccga cgtgcatcat cggcatcacg ttagaggtgg tggtggaact catcagatcc
     1261 cacaatgaac cgtttttgag gaatttggtt gagaaggtgg caaatggccc aaacagcacg
     1321 acgcgtttac cgtccagaac acgggtatcg atatgcggaa ccgacatcgg tggtgcgcca
     1381 acggatgctt taccgtaaac cttcgccaga tggtgattaa ccacgtccgg gttttccgaa
     1441 acaaggaact gtccgcccac cgggaaaccg gcgtagtctt tcgcttccgg aatccccgat
     1501 tcctgtaaca gcttcagcgc cgcaccgccc gcgccgataa agacaaattt ggcgcggatg
     1561 ttctgtgcag tgccattttt cagatcggca acagtgacgg tccaggtgtt atcgtcatta
     1621 cgctttaggg cgcggacttc gctgctgagt tgcagcgaaa aattagattt cttctgcaag
     1681 gaagcaatta actggcgggt gatctcgcca tagttcacat cagtaccaat ttccgtacgc
     1741 gtggctgcca ctttctgttg cggatcgcgc ccttccatca ctaacggtgc ccactctttg
     1801 atctgcgcgt gatcttcaga gtaacgcata ccgcgaaaca gcgagctttg ttgcaacgcg
     1861 gcgtaacggg cgcgcaggaa attgacgtta tcctcgcccc agacaaagct catatgcgga
     1921 acggtattga taaatgaacg cggagtacgc agcacgccgc gctcgacctg gtgcgcccag
     1981 aactggcgcg aaatctgaaa tgcttcgtta atggcgactg ctttttcaat actgatgctg
     2041 ccatcggcgt tttgcggagt gtaattgagt tccatcagtg cagaatgccc ggttccggcg
     2101 ttgttccagc cgttcgaact ctcctgcgcg acaccctcca ggcgctccac catggtcatc
     2161 gaccattcag gctccagctc gcgtaaatag gtccccaacg tggcgctcat aatgccgccg
     2221 ccaatcaaca gtacatcagt ttcctgctcc tcggatgctt tcgcttttgc cgccatcgaa
     2281 acggcattaa gccccacggc catcgagaag agcatggcag tcactttttt catcttgtta
     2341 atgccttact tttagtcgct ttattgcagg tgagatttgc gcggcatcaa cggt
//
LOCUS       DAZUCC010000385         2366 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZUCC010000385 DAZUCC010000000
VERSION     DAZUCC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.387"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..824
                     /gene="iolR"
                     /locus_tag="ACHY09_005081"
     CDS             <1..824
                     /gene="iolR"
                     /locus_tag="ACHY09_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator IolR"
                     /protein_id="HFS3887898.1"
                     /translation="QTQLSLLQDDIRSRYDSLSKRLKQVAQYILDNSNSVAFDTVASI
                     AQHAEVPPSTLIRFANAFGFSGFNEMKQVFRQHLMEETVSYTERARLFRQKNINDGEP
                     TPEKPGEILKLFSMVNAQVLQQLPMQIKNEQLDAAVSLLSKADNIYVIGLRRSFSVAC
                     YLTYALRHLERRAFLIDGIGGMFSEQLSMISSNDVVIAISYSPYAQEVVELVELGAKR
                     DAHHIVITDSQVSPLAAFSEVCFVIREAQVDGFRSQVASMCLAQTLAVSLALAAE"
     gene            complement(877..2310)
                     /gene="iolT1"
                     /locus_tag="ACHY09_005082"
     CDS             complement(877..2310)
                     /gene="iolT1"
                     /locus_tag="ACHY09_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol import MFS transporter IolT1"
                     /protein_id="HFS3887899.1"
                     /translation="MPISVNRYNTGYILRICSIAALGGILFGYDTAVISGAISSLTSY
                     FHLSPAETGWAVSSVVVGCVVGSFSSGYLSKRFGRKKSLMISALLFTISAVGTSLSYT
                     FLHFIIYRIVGGLAVGLAATVSPMYMSEVSPKDMRGRALSMQQFAIVFGQILIFYVNY
                     KIASIATNTWLIEIGWRYMFAAGIAPCILFCILVFFIPESPRWMMMAGREEETLKILT
                     KISNEEHARHLLTDIKTSLQNDRLTAHQKLNYHDSNVRFILFLGCMIAMLQQVTGVNV
                     MMYYAPIVLRDVTGSAQEALFQTIWIGVIQLVGSIIGAMIMDKMGRLSLMRKGTIGSI
                     IGLLLTSWALYSQTTGYYALFGMLFFMIFYALSWGVGAWVLISEIFPNRMRSQGMSIS
                     VGFMWIANFMVSQFFPIINENPWLLAHFHGAFPMWIFAACCVFSYFFICRYLPETKGV
                     SLEKMEDIVLTKRRNKPQPIQAERSSD"
BASE COUNT          689 a          500 c          547 g          630 t
ORIGIN      
        1 accaaacaca gctttcctta ctgcaggacg acatccgcag tcgctatgac agtctcagca
       61 aacgtcttaa acaagttgct cagtatattc tggataacag taatagcgtt gcttttgata
      121 cggttgcttc tattgcgcag cacgcggagg tacctccttc gacattaatt cgttttgcca
      181 atgcgtttgg ttttagcgga ttcaatgaaa tgaaacaagt atttcgccag catttgatgg
      241 aggaaaccgt tagctataca gaacgggctc gtctgttcag acaaaaaaac atcaatgatg
      301 gggaacccac accggaaaaa ccgggcgaaa ttctgaagct attttccatg gtaaatgcac
      361 aggtactaca acaattaccg atgcagatta aaaatgagca actggatgct gcagtaagtt
      421 tattgtcaaa agccgataat atttatgtga ttggattacg tcgttcattt agcgtggcct
      481 gttatttaac ctatgcattg cgccatcttg agaggcgtgc atttttaatt gatggtattg
      541 gaggcatgtt ttctgagcaa ctgagtatga tcagctctaa cgatgtagtc atcgccatca
      601 gttattcgcc atacgctcag gaggttgttg aactggttga gttgggggca aaacgtgatg
      661 cacatcatat cgtcattacc gacagtcagg tcagtccgtt ggctgcgttc agtgaggtct
      721 gttttgtaat acgtgaagcg caggtggatg gcttccgttc gcaggtagcg tcgatgtgtc
      781 tggcgcagac gttggccgtc tcactggctc tggctgctga ataagaggaa gatgccgata
      841 atctgttttt catgatcatc ggcattttgg ctaagcttaa tctgaagagc gttcagcctg
      901 aataggttgt ggcttgttcc tgcgttttgt cagcacgata tcctccattt tctctagtga
      961 gacacctttg gtttccggca ggtagcggca aataaagaaa tagctaaaga cacagcaggc
     1021 tgcgaatatc cacattggaa atgcaccgtg gaaatgggcc agtaaccagg gattttcgtt
     1081 aattatagga aagaactgtg aaaccataaa gtttgctatc cacataaatc ctacagaaat
     1141 actcattccc tgtgaacgca tgcggttggg gaatatttct gatatgagca cccatgctcc
     1201 aacgccccag gaaagtgcat agaaaatcat aaagaacagc ataccaaaga gagcataata
     1261 gccggttgtt tggctgtaca gagcccatga agttaataat aaaccaatga tgctaccaat
     1321 ggtgcctttt cgcattagcg ataaacgccc cattttgtcc atgatcattg cgccaataat
     1381 tgatccgacg agttgaatta cgccaatcca gatggtctga aacagcgctt cctgggcgct
     1441 accggtaaca tctctcaata caataggtgc gtaatacatc ataacgttaa cgccggttac
     1501 ttgctgtaac attgcgatca tgcaaccgag gaaaaggata aaacgtacgt tactgtcatg
     1561 gtaattgagc ttttggtggg ccgtcagccg gtcattttgc agagaggttt ttatatctgt
     1621 tagcaaatgt cgtgcatgtt cctcgttaga tatctttgta agaattttta gcgtttcttc
     1681 ttcacgacca gccatcatca tccagcgggg ggattccggt ataaagaata ctaaaataca
     1741 gaacagaatg catggggcaa tgccggcagc aaacatataa cgccagccaa tctcaatcag
     1801 ccatgtattg gtcgcaatcg aggcgatttt atagttgacg taaaatatga ggatctgacc
     1861 aaaaacgatg gcaaactgtt gcatactcaa tgccctgcca cgcatgtcct taggtgaaac
     1921 ctccgacata tacattggag atactgttgc tgcgaggccg acagcaagtc cacctacgat
     1981 acgataaata ataaagtgca ggaaggtgta ggaaagagat gttcccactg ctgagattgt
     2041 gaacagcagg gcggaaatca tgagtgattt cttgcgacca aaacgtttgg ataagtaacc
     2101 tgagctgaat gatcccacca cacaacctac aacgacgctg gaaaccgccc agccggtttc
     2161 ggccggagat aggtgaaaat aactggtgag agaactgatt gcacctgaaa taacggcagt
     2221 atcgtagcca aataatatcc cgcctaatgc tgcaatagaa cagatacgta atatatagcc
     2281 tgtattgtaa cgattcactg atatgggcat agctgattat ctcctgagca gaaatcatct
     2341 atctatttga cctataatga aaaaat
//
LOCUS       DAZUCC010000386         2353 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZUCC010000386 DAZUCC010000000
VERSION     DAZUCC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.388"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..250
                     /locus_tag="ACHY09_005083"
     CDS             <1..250
                     /locus_tag="ACHY09_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFS3887900.1"
                     /translation="HDVPYLIAKKRNHPHADAIHVAGWVSLFTLHVIWPFLWIWATLY
                     RPERGWGMQNHDSSVVQLQQRIAGLEKQLADIKSSSAE"
     gene            256..1392
                     /locus_tag="ACHY09_005084"
     CDS             256..1392
                     /locus_tag="ACHY09_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS3887901.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSAAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1389..>2353)
                     /locus_tag="ACHY09_005085"
     CDS             complement(1389..>2353)
                     /locus_tag="ACHY09_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3887902.1"
                     /translation="QLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFESKV
                     GLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGI
                     ITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDESLVA
                     SFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVR
                     DYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLV
                     AIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          582 a          561 c          602 g          608 t
ORIGIN      
        1 acatgatgtt ccctacctga ttgccaaaaa acgtaatcat cctcatgccg acgccattca
       61 tgttgctggt tgggtgagtc tttttacgtt acatgttatc tggccatttt tgtggatttg
      121 ggccacgctt taccgcccgg agcgtggatg gggaatgcaa aaccatgact cctccgttgt
      181 gcaactacaa cagcgtattg ccgggctgga gaaacagctc gctgacatca aatcctcttc
      241 tgccgagtaa tacttatgga tttactgatt attttaacct atgtggcttt cgcatgggca
      301 atgtttaaga tcttcaaaat tcctgtaaat aaatggacca ttcccacagc ggccctggga
      361 ggcatattta ttgtcagcgg tctaattctg ttaatgaact ataaccatcc gtataccttt
      421 aaagcgcaaa aagcggttat ttctattcct gttgtcccac aggtgacagg cgtggtgatc
      481 gaagtgacgg acaagaaaaa tacgctgatt aaaaaaggtg aggtgctatt tcgactggac
      541 ccgacgcgtt atcaggctcg ggtggatcgg ctgatggcgg atatcgttac cgcagaacat
      601 aaacagcggg cgttgggcgc agagttagat gagatggcgg cgaatactca gcaggcaaag
      661 gccacgcggg ataagttcgc taaagagtat cagcgttacg cacgtggcag ccaggcgaaa
      721 gtaaacccgt tttcagaacg cgatatcgat gtggcgcggc aaaattatct ggcgcaggaa
      781 gcctccgtaa aatcagcggc ggcggaacaa aaacagatcc agagccagct ggatagcctg
      841 gtgttgggtg aacattctca aatcgccagc ctgaaagcgc agctcgcgga agcaaaatat
      901 aaccttgagc agacgatagt gcgtgcgccg agcgatggtt atgtgaccca ggtgctgatt
      961 cgtccgggta cctatgccgc gtcgctgccg ctacgtccgg tgatggtgtt tatacccgat
     1021 cagaaacgac aaatcgtggc gcagttccgt cagaactcct tgctgcgcct ggctcccggc
     1081 gatgatgcgg aagtggtatt taacgctctg ccaggcaaag tgttcagcgg taagctggca
     1141 gccattagtc cagctgttcc cggtggagct tatcagtcga ccggcacctt acagacgtta
     1201 aacacagcgc cgggttcaga tggcgttatc gcgaccattg aactggatga gcatactgat
     1261 ttgagcgcat taccagacgg tatttacgcc caggtggcgg tctattctga tcatttcagc
     1321 catgtctcgg tgatgcgcaa agtactgtta cgcatgacca gctgggtgca ttacctttat
     1381 ctcgatcatt aatcttcgct ttccgccgct tgttcaggcc gcagttgctg cgactgagca
     1441 agctggtggc gaatttcacg cagcatttca tccagcacat actggacccg ccagggctgg
     1501 tattcccgtt tacggaagat agccaccaga tccagccacc attcatacgg gtgctgaaag
     1561 cagggcacca gcgtgccgtc ttgcaggtcg ctctgcacac ttttgtctgg cgcaaacaca
     1621 atccccaggt gatttcgtgc cagctccagc gcagattgcg tgttgtcgca aacgtaattt
     1681 cccttcacgc gataatcccg cacctcttta ctcccggcga cgttgaagcg ccagatattc
     1741 gcgtcgtcga tcatcatcga gtcgatcaaa atacaggagt gatgttcgag ttcgtcagga
     1801 cggctaatgg gatgtttctc aagatagcgc tggctggcat aagcggttac ggcatattgg
     1861 gtaataaaac tggcaaccag cgattcatct tttggttggg cgtagctgat taaaacatca
     1921 caatcatcgg gaaattcgac gccttcataa aaggcattac gctcaagatt gcaggttttt
     1981 agcgataagg tgatatcacc gatatcttta attttatcga ttacatgttt ggataaatag
     2041 gtaattatgc cggttggtgc atagatagtg acccgaccac gtttctcatg cttataatct
     2101 gcaataaaat tattaagctg ctcgtttctg tccagcatgt cgttgatata cggtaacagc
     2161 acggtgccaa aaggtgtgag catcagctgt cgggtggttc ggtcaaagac ttttaaaccc
     2221 acttttgatt caaaatcagc aagatatttg ctgacgttgg cctgtgcgat gccaagtaca
     2281 gtcgcggcat ggctgatatt ttcactggca gcgattaccg agataatttt taactcccgg
     2341 tacttaagtt gta
//
LOCUS       DAZUCC010000387         2325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZUCC010000387 DAZUCC010000000
VERSION     DAZUCC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.389"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..378
                     /locus_tag="ACHY09_005086"
     CDS             <1..378
                     /locus_tag="ACHY09_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3887903.1"
                     /translation="VAPIMMEQNHGAIITMSSVVGTDGNIGQSNYAATKGGVIAMSKG
                     WAKEFSRKGAKVRVNCVAPGFVITPMTENLPEKVINHMIAKTPLGRMATPEDISNGVL
                     FLASEKSSFITGQTLKIDGGLVI"
     gene            388..1596
                     /locus_tag="ACHY09_005087"
     CDS             388..1596
                     /locus_tag="ACHY09_005087"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008913448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFS3887904.1"
                     /translation="MEKVYITGAKRTPIGCFGGSLSTVSASDLGAIAISAAIKQSRIN
                     VECIDEVIVGNVLSAGQGMGPGRQAAVKAGIPWKTPALTLNMICGSGMKTIIDAAMKI
                     RSGDMDVVVTAGIENMSQAPYLIDHHARSGVRMGNQVMSDSLITDGLTDAFNHYHMGI
                     TAENIARQFNITRYEQDTFAFESQKRALKAIDNGEFEDEITPVQVHTRKGNIEVTTDE
                     YPRRDSSLEKLSVLRPAFEKNGTVTAGNSSGINDGAAALILASEKAIQTLELQPLAEI
                     RSFGQGGVDASVMGLGPVPAIDNALKKAKLDIKDIDLFEINEAFAAQAIGVLKSISEN
                     HSVSIDWLNQRTNVNGGAIALGHPLGASGSRIVVSLLYQMIKNNKKRGLASLCIGGGM
                     GTAVIIELCS"
     gene            1666..>2325
                     /locus_tag="ACHY09_005088"
     CDS             1666..>2325
                     /locus_tag="ACHY09_005088"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887905.1"
                     /translation="MIGLIISLILLIYLIYRGFPVLIVSPLMAMLAVFMGSDIPVFYS
                     WTGPFMDSVVSFISAYFPIFLVGAVFGTLMNASGAALTIAHFISDKMGEKRAIIAVII
                     ASFLLTYGGISVFVVVFAVLPIARTIFIKSNYPQRLLPSAICAGCLPAYVAPGSAQFI
                     NAIPIPVFNTTIYSGLVIGIVGTAVWLLFAVWYMSAKIKNAKIKGEGYGTEVSNDCNL
                     ET"
BASE COUNT          717 a          350 c          521 g          737 t
ORIGIN      
        1 gttgcaccta ttatgatgga acaaaatcat ggggcaatca taacaatgtc ttctgttgta
       61 ggaactgatg gtaatatagg tcagagtaat tatgccgcaa ctaaaggtgg tgttattgca
      121 atgtctaaag gttgggctaa agaattttcc cgaaaaggtg ctaaagtacg cgtgaattgt
      181 gtagctccag gattcgttat taccccgatg acagaaaatc tgccagagaa agtcattaac
      241 catatgatag ctaaaacccc tttagggcga atggcaacac ctgaggatat ttcgaatggt
      301 gttctttttt tagctagtga gaaatcatct tttattactg gtcaaacatt aaaaattgat
      361 ggtggattag ttatttaagg aggaggaatg gaaaaagtat acattacagg ggcaaaaaga
      421 acgcctatag gatgttttgg cggttcgtta tcaacagtgt cagcttcaga tcttggagca
      481 attgcgatat ctgcagcaat taaacagagc agaataaacg ttgaatgtat tgatgaagtt
      541 attgtcggga atgttttgtc tgctggacaa ggaatggggc caggtagaca agctgctgtt
      601 aaagccggta taccatggaa aactccggct ctgactttga atatgatttg tggtagtggt
      661 atgaaaacta ttatcgatgc tgcaatgaaa attcgtagtg gagatatgga tgttgttgtt
      721 acggcaggca tagagaatat gagtcaggca ccttacttga ttgatcatca tgcaagaagt
      781 ggtgtacgaa tgggtaatca ggtaatgagt gactctctta ttacagatgg tcttactgat
      841 gcttttaatc actatcatat ggggattaca gcagaaaata tagcccgaca gttcaatatt
      901 acaagatatg aacaggatac atttgcgttt gaaagtcaga agagagcctt aaaggccatt
      961 gacaatggag aatttgagga tgaaattact ccggtacaag tacatacccg aaaaggaaat
     1021 attgaagtta ctacagatga gtatcctcgt agagactcct cattagagaa actttctgtg
     1081 ttacgtccag catttgaaaa aaatggtacc gtgactgcag gaaattcatc tggaataaac
     1141 gatggtgcag cagctcttat tcttgcttca gagaaagcaa ttcagacatt ggaattacaa
     1201 cctttggctg aaattcgctc atttggacaa ggaggcgttg atgcatcagt gatggggctg
     1261 gggcctgttc cagcaataga taatgcatta aaaaaagcaa aactggatat taaagatatt
     1321 gatctttttg aaattaacga ggcgtttgcg gctcaggcaa taggggtctt aaaaagtatt
     1381 tctgaaaatc actcagttag tattgattgg ctaaatcaaa gaacaaatgt taatggagga
     1441 gcgatagctc taggccatcc tttgggagcc tctggctcaa gaattgttgt ttctttatta
     1501 tatcaaatga taaaaaataa taaaaaaaga ggtctcgctt ccctttgtat tggtggagga
     1561 atggggacag ctgttattat cgaattgtgc agttaacttt gaaataagta tctcttgctt
     1621 gtcaggagat acttaaataa taatattaat attgagagtt ataatatgat aggcttgata
     1681 atttcactta ttttactaat atatctcata tatcgtggtt ttccagttct gatagtatcc
     1741 cctttgatgg caatgctggc tgtctttatg ggaagtgata ttcctgtctt ttattcatgg
     1801 accggtccat ttatggactc ggttgtgagt tttatatccg catacttccc tatatttctt
     1861 gttggtgctg tttttggaac attaatgaat gcaagtggag cagcattaac tattgcccat
     1921 tttatatcag ataaaatggg tgagaagagg gctattattg cagttattat tgcatcattt
     1981 ttattaactt atggaggaat atcagttttt gttgtggttt ttgcagtttt acctattgcg
     2041 agaactatat ttataaaaag taattaccct caaagattat tacccagtgc tatctgtgct
     2101 ggttgcttac cagcatatgt agctcctggt tctgcccagt ttataaatgc aataccaatt
     2161 cctgtgttca atacaaccat atattcaggt ttagtaattg gtatcgttgg cactgctgta
     2221 tggttattgt tcgctgtatg gtatatgtct gcaaaaatta aaaatgctaa gataaaagga
     2281 gaaggatatg gtacagaagt aagtaatgac tgtaatcttg agact
//
LOCUS       DAZUCC010000388         2274 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZUCC010000388 DAZUCC010000000
VERSION     DAZUCC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.390"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(143..1666)
                     /locus_tag="ACHY09_005089"
     CDS             complement(143..1666)
                     /locus_tag="ACHY09_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS3887906.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIREALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTRSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2002..2250)
                     /gene="ymgC"
                     /locus_tag="ACHY09_005090"
     CDS             complement(2002..2250)
                     /gene="ymgC"
                     /locus_tag="ACHY09_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFS3887907.1"
                     /translation="MNNSIPERFILQCALFKNLEREVFMTHGYVDSYIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVIS"
BASE COUNT          683 a          509 c          470 g          612 t
ORIGIN      
        1 ctattgttca tgctactaat ggcaatcctg tcaaaacttt caaacaatta tagcaaacaa
       61 aataatgaac aatagtaaca agaggcaacg agctaattag ctgtctataa ttacaccgca
      121 ctatggcctt actgatcgat tttcactcaa ccacaacctt caccttcggt ttagaaagga
      181 taattttgac caggtcgatg tatgtaacag gtttatagaa ataataaccc tgctgaaatg
      241 tgatattatt ttgattcaga tagtcaagtt gttctttcgt ttcgacacct tcagcgacga
      301 tactgatcga aagtttacgc gccagatcca atacacaatc cagaattcgg gttgattctg
      361 ggtctgcgtt gacgcggcca acgaaactgt gatcaatttt gatataatca atatgcaggt
      421 catgcagata agaaagccct gagtagccag taccgaaatc atccagcgcg atgacaaaac
      481 cattttcatg cagaatgttc aaccgctgaa ccagactttc atcaacattt aatggctcac
      541 gctcggtaac ttccagaacg aggtttaaat cgcggcgggt aaaactgtca cggtaattta
      601 agcactcgtc gacaaacgtc ggcgaaataa tatgcgaggc gctaaaatta attccaatat
      661 gaaaaccttc cggcaattta ctcgcgatag cgttcatctg tctggcaacc tgattcatca
      721 ggcttcgcgt aagcggaacg attaatccgg atttttcagc aagtggaata aatgccgcag
      781 gtgatatata tccaccgtga ggttgtttcc agcgggctaa cacctcaact ccccgcaatg
      841 tcccttcccg accatttacc acaggttggt aaaaagggac gatctcccca ttatttatag
      901 cccgtcgcag gatctcttca ggcgttgcgc ttttattgaa atacctatca agcaggaagg
      961 cagcggcgca ggcgattaac agaataaata tcaaaatccc aaagccttta tcgataagtc
     1021 tgtagaagct aaagagtggc ggcgggttaa agataatagt gaaatgatat tttgtagagt
     1081 gaaccttctc ggtataggga tagtgtccat taagcgtcat cacatcaccc gtaggtccaa
     1141 taatgtcatc tgccacgcgt aatacatacc ttaccccttt caagggtaca ttaagcgcct
     1201 cgcgaatatg ctgatcactt atcgtaacca aaatgcgcgt atctgcaaat tggctctgat
     1261 agagcaggat aggtaatcta ttaacggtgt cgattgccgg agccagcagt aaattactgt
     1321 ccgggaaaac aggaatacga gacagtagga cccgattccc cggtaaggat gtacaccaga
     1381 ccgttccctg tttgagaatg atgattgtgc gcaggtgagg cttcagagct gcttcagtgc
     1441 caagctgata ttgctcctcg aggtcgcatt ccttcccggc aatgttaacc gccgtttgag
     1501 tcgcctgtga cgcttcttca agtatatgat tgatattgtt ggcggcatat ctcgctcccg
     1561 ccaggtactc tgctttcgcg gaataccaga gctggatgtt caaaatagcg acaccggtta
     1621 taaacaagca tatcgcaacg aggatgggta tgagtgtatt gcgcattcgc ttgtcccgga
     1681 caaagggttt aatacatgaa aatgacgttc attcgctcgc gctggcgtct acagagagca
     1741 tggtagagag catcacaaat atccacgccg aagataaaat tttctccaac ttcatctaac
     1801 ctattaatta ttgagcacaa tgtaggcaga attaaacaaa ttaggtggga tatcaggcgt
     1861 caagaatgaa aaacgagcgc tctccattcc tgtttgcccc ctgatattgc ggatatatta
     1921 agaaagcata acgcctgaaa tgctcacttt gcaccagcat ggtgatacag ctgatgttta
     1981 ttctataacc ttactcaagt tctaagagat cacggattcc ctgtcattaa taatgatatc
     2041 cgttagtgta gtcttatgca tttcaatgta ctgcaatatt tgcagataga gatcagaaag
     2101 aatcacactg gtttcatctt ttaaacgcaa gcgtaaagcc tgatcaataa tataactatc
     2161 aacataccca tgcgtcatga acacctccct ctcgagattt ttaaacaaag cacattgcaa
     2221 aataaatctc tctgggattg aattattcat gtcagcctcg tagaaatatt aacc
//
LOCUS       DAZUCC010000389         2263 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZUCC010000389 DAZUCC010000000
VERSION     DAZUCC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.391"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            131..634
                     /locus_tag="ACHY09_005091"
     CDS             131..634
                     /locus_tag="ACHY09_005091"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3887908.1"
                     /translation="MNNIAPQSPVIRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(680..1096)
                     /gene="rraB"
                     /locus_tag="ACHY09_005092"
     CDS             complement(680..1096)
                     /gene="rraB"
                     /locus_tag="ACHY09_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFS3887909.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1258..>2263
                     /gene="argF"
                     /locus_tag="ACHY09_005093"
     CDS             1258..>2263
                     /gene="argF"
                     /locus_tag="ACHY09_005093"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS3887910.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     L"
BASE COUNT          583 a          533 c          560 g          587 t
ORIGIN      
        1 tgccacgtta caatactctt atcagtaata ggctggcaaa aactttattg ctttataaga
       61 cgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt ataattactt tatttttatt
      121 gggttaaact atgaataaca ttgcgccgca atcacctgta atacgtcgcc tgacgctgca
      181 ggataatcct gctatcgccc gcgtcattcg tcaggtatcc gccgaatacg gtcttaccgc
      241 tgataaaggc tacaccgtcg ccgatccgaa tcttgacgag ctgtatcaag tgtatagtca
      301 gcctggccat gcatattggg tggttgagta cgagggtgaa gtggtcggcg gcggtgggat
      361 agcgccatta acaggtagtg aagcggacat ttgcgaactg cagaaaatgt actttcttcc
      421 cgccattcgc ggtaaagggc tggcaaaaaa actggcctta atggcgatgg agcaggcgcg
      481 agaaatgggt ttcaaacgct gctatctgga aacgaccgct tttttgaaag aagcgattgg
      541 tttatatgaa catttaggtt ttcaacatat cgactatgca cttggctgca cgggccatgt
      601 tgattgtgaa gtgcggatgt tgcgcgaact ctgaaaaaat gcccgctagt atgtgctatc
      661 gggcatttta atcaattaat tagtggcgaa ccccgtcatc gtcttcatcg acaaaatctt
      721 catcgtcgcc atcttcgccg ttaggatctt caaagtaagt gccccaaccg tcgtactcaa
      781 cgtcaaattt ctccgccagc gtcatcagtt gttcaacctg ggcatcgatc agatcggcat
      841 tcaacgcgca ctcgctgagg atgtcgcagc aaatcacgat atcaccatct tcaacttcca
      901 gctcttctgg atcggtcact tcgtaaccga gtttaaacgc ttcaactgcc gctttttcca
      961 gagtttccag atcgtctgcg gaaagatggt gttcgatggt gtacagcgcg tccggatcgc
     1021 tgccatcttc cagtaattct tcaataatca aacgtgtttc ttcacgctgt tcttccagtt
     1081 gttccgggtt tgccatggct cattcctctt aatgtcctgc cgatactctt attgtcacac
     1141 accactgaca ttgcctccac ctttgtgata aagatttatg ctttagactt gcaaatgaat
     1201 aatcatccat ataaattgaa ttttaattca ttgaggcgtt agccacagga gggatctatg
     1261 tccgggtttt atcataagca tttcctgaaa ttactcgatt tcacgccagc tgaacttaac
     1321 agcctgctgc agttagccgc gaagctgaaa gctgataaga aaagcggtaa agaagaagcc
     1381 aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag actcgacccg tacccgatgc
     1441 tctttcgaag ttgccgcata tgaccagggt gctcgcgtta cttatctcgg cccaagcggt
     1501 agtcagattg gtcataaaga gtcgattaaa gacactgccc gcgtgcttgg tcgcatgtat
     1561 gacggtattc agtatcgcgg ctatggtcag gagattgtcg aaacactggc ggaatacgct
     1621 ggcgtgccgg tatggaacgg cctgaccaat gagtttcacc ccacgcagtt gctggcggat
     1681 ctcctcacca tgcaggagca tttacccggt aaagcattca acgaaatgac gctggtctat
     1741 gcaggtgacg cacgtaacaa catgggcaat tcgatgctcg aagctgcggc gcttaccggt
     1801 ctggatttgc gtctggtcgc gccacaggca tgctggccag aagctgcgct ggttacggaa
     1861 tgccgcgccc tggcacaaca aaatggcggg aatattacgc tgacggaaga tgtagctaaa
     1921 ggagttgaag gtgctgactt tatctatacc gatgtatggg tgtcgatggg tgaagcaaaa
     1981 gaaaaatggg ctgaacgcat tgcattactg cgtgattatc aggtgaacag caagatgatg
     2041 cagttgaccg gtaacccgga ggtcaaattc ctccactgcc tgcccgcgtt tcatgacgac
     2101 cagacgacgc ttggtaagaa aatggcggaa gagtttggcc tacatggcgg aatggaagtg
     2161 actgatgagg tcttcgaatc tgccgccagc attgtttttg atcaggcgga aaaccgcatg
     2221 cataccatca aagcggtgat ggtcgcgacg ctcagtaaat taa
//
LOCUS       DAZUCC010000390         2242 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZUCC010000390 DAZUCC010000000
VERSION     DAZUCC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.392"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            119..1636
                     /gene="lysS"
                     /locus_tag="ACHY09_005094"
     CDS             119..1636
                     /gene="lysS"
                     /locus_tag="ACHY09_005094"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS3887911.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(1755..1928)
                     /gene="ghoT"
                     /locus_tag="ACHY09_005095"
     CDS             complement(1755..1928)
                     /gene="ghoT"
                     /locus_tag="ACHY09_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFS3887912.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(1956..>2242)
                     /gene="ghoS"
                     /locus_tag="ACHY09_005096"
     CDS             complement(1956..>2242)
                     /gene="ghoS"
                     /locus_tag="ACHY09_005096"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFS3887913.1"
                     /translation="NKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPR
                     QLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
BASE COUNT          645 a          469 c          520 g          608 t
ORIGIN      
        1 ggtaagcgtt agtttcgata agataaactg agttattaat agtcgaggca gataatacag
       61 tgtaccgatc tgtctcttta tctacgctaa attgaaagct ggatttagag gaaccaaaat
      121 gtctgaacga gaaacacggg gagccaatga ggctattgat tttaacgatg aactgagaaa
      181 tcgccgcgaa aaactggcgg cactacgtca gcaaggtgtg gcgtttccca atgattttcg
      241 ccgcgaccat acctctgacc agttgcacga agagtttgat gcgaaggata accaggaact
      301 ggaatcctta aacattgaag tctcggttgc tggccgaatg atgacccgtc gtattatggg
      361 gaaagcctcc tttgtaacgt tgcaggatgt cggtggccgt attcaactgt acgttgcaag
      421 agatagcctg ccagaaggtg tttataacga tcagtttaaa aaatgggatc tgggtgacat
      481 tatcggtgcc cgcggtacgc tgtttaagac gcaaacgggt gagctttcca ttcactgtac
      541 tgagctgcgc ctgctgacta aagcactacg tcctttacca gataaattcc atggtctgca
      601 ggatcaggaa gtccgttatc gtcaacgtta tctggacctc atcgctaacg ataaatcccg
      661 tcaaacgttt gttgtccgtt caaaaattct ggccgctatc cgtcagttca tggtcgctcg
      721 cggctttatg gaagtagaaa ccccgatgat gcaggtaatt ccaggtggag catctgctcg
      781 cccgtttatt acccatcata acgctctgga tttagatatg tacctgcgta tcgcgccgga
      841 gctatatctg aaacgtctgg ttgtaggtgg ttttgaacgg gtattcgaaa tcaaccgtaa
      901 cttccgtaat gaaggtattt ctgttcgtca taatcctgag ttcacgatga tggaactcta
      961 catggcgtat gcggattacc acgatttgat tgaactgacg gaatcactgt tccgcactct
     1021 ggcacaagag gtactgggta ccactaaagt cacttatggc gagcatgtgt ttgatttcgg
     1081 caaaccgttt gaaaaactca ccatgcgcga agcaatcaaa aaatatcgtc cagaaaccga
     1141 tatggcggat ctggataatt ttgatgctgc taaagcatta gctgaatcta tcggaattac
     1201 ggtagagaaa agctgggggt tgggacgcat tgtcacggag atctttgatg aagtggcaga
     1261 agcacatctg attcagccaa cctttatcac ggaatatccg gcagaagtgt caccgctggc
     1321 gcgccgtaat gatgttaacc cggaaatcac cgaccgtttt gaattcttca tcggtggtcg
     1381 tgaaattggt aatggtttta gcgaattaaa cgacgcagaa gatcaggctg aacgtttcca
     1441 ggaacaggtt aacgctaaag ctgcaggtga cgacgaagcc atgttctatg acgaagatta
     1501 cgtaactgcg ctggaatatg gtctgccgcc aaccgctggt ctgggtattg gtatcgaccg
     1561 aatgattatg ctgtttacaa acagtcatac tattcgcgac gttattctct tcccggcgat
     1621 gcgcccactg aaataaattt cactttaatg aacgaagcag tcaggcgact gcttcgttcg
     1681 tcattccgca aatccagttt tgaggagaga cgcttcgttt caagataacc agcgggtcca
     1741 ctcaactaca gctcctaaaa gagagaaaaa agtaatgcca caggcagact cattggccag
     1801 gttattccga ccagaaatgc actaagtaaa cgaatatgtg ttttctcatg tgagataaac
     1861 cagataatga caaaggatat gttcacacca atcacataaa aaattaatat tttagagaat
     1921 agtgccattt tttatgttct tcttatcctt cctggctact tgtaaaactg acggaaatag
     1981 tcatcaactg tcataatgtt gaggtcaaag tcaatatccg gtaagtctaa gcatttaagc
     2041 ctgattaaat cttcgatttc acttgcggct aacgatgtcg ttattgcgaa ggagttttca
     2101 ttcagttgtc gtggtatccc atattcatca gccacgatag aggagaaact catatcatac
     2161 atcaatgatc ttaaccttaa gaacactgag gaataagatg atggataatc aaaagaaaca
     2221 acataagtat tgaacttgtt tt
//
LOCUS       DAZUCC010000391         2235 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZUCC010000391 DAZUCC010000000
VERSION     DAZUCC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.393"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1571
                     /locus_tag="ACHY09_005097"
     CDS             <1..1571
                     /locus_tag="ACHY09_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFS3887914.1"
                     /translation="DHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIPA
                     NAGILLVPCCRGASAFTTGADGTYSESAGASENSLRWGVGKPLYQDLVSRTKAALAKN
                     PKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQFRTELAGQASQSTGGSASAVPWI
                     CGDTTYFWKQRHAEGYASVYGGYKGKESQNIFFVPLMTDENGANVQTNNPAEDPDLEA
                     VGYYGSKWRNDQKTWTSADRASHFSSWARRGIISDRLATAILVHAGRTAEFITGKQPD
                     TVKPTGPSGEGPEREPEAPVSNRTLMSLLASGEDLASQGWRYYHKPASGDSVNKNIAE
                     AVVSDEGATGGKALQLNKPESHIWFLEHDAAGQGAELLKKGGRVSVRFKVPGALVPNQ
                     FALGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATNLNAMYHKKQNAKLDTFGVFDN
                     GWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGAADKLQLTDISKAATYTLLI
                     DSVKVEVNNADAAA"
     gene            1722..1904
                     /locus_tag="ACHY09_005098"
     CDS             1722..1904
                     /locus_tag="ACHY09_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFS3887915.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SASQSAGSKPEEPITP"
     gene            1930..2175
                     /locus_tag="ACHY09_005099"
     CDS             1930..2175
                     /locus_tag="ACHY09_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFS3887916.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
BASE COUNT          564 a          523 c          700 g          448 t
ORIGIN      
        1 cggaccattg tctgcatgat gtgcaggaca tgagccgtct taaccatccg aaagcggacc
       61 tgtcaaaggg gcagtacgga accgtggggc aggggctgca tatcgccaaa aaactgctgc
      121 cgtttatacc ggcgaatgcg ggcattctgc tggttccgtg ctgccgtggt gcttcggcat
      181 tcacaacggg tgcagacggc acatacagcg aatcagccgg tgcatcggaa aattcactgc
      241 gctggggtgt gggtaagccg ctgtatcagg atttggtgag ccggactaaa gccgcactgg
      301 cgaagaaccc gaaaaaccgt ctgcttgcgg tggtgtggat gcagggagaa ggtgatgcgg
      361 cagtggggac gcatgcgcag catccggggc tgtttagtgc gatggtgaat cagttcagaa
      421 cggagcttgc cggtcaggca tcacagagca caggaggcag cgcatcagcg gtaccgtgga
      481 tttgcgggga cacaacgtat ttctggaagc agcgtcatgc ggaagggtat gcatcagtat
      541 acggagggta taaggggaag gagtcgcaga acattttctt tgtaccgtta atgacggatg
      601 agaatggtgc gaatgtgcag accaataacc cggcagaaga cccggacctg gaagccgtcg
      661 gttattacgg ttcaaagtgg cgtaatgacc agaagacctg gacatcagca gacagggcca
      721 gccatttcag ttcatgggct cgccgtggga ttatttccga ccgtctggca acggcgattc
      781 tggtgcatgc cgggagaact gctgaattca ttaccggaaa acagcctgat acggtgaagc
      841 ccaccggacc ttccggtgaa ggcccggaga gagagccgga agccccggtc agtaaccgaa
      901 ccctgatgag tctgctggcg tccggtgaag acctggcatc acagggctgg cgctattatc
      961 acaaaccggc gagcggagac agtgttaata aaaacatcgc tgaggctgtg gtcagtgatg
     1021 agggtgccac cggtggtaag gccctgcaac tgaacaaacc ggaaagccac atctggtttc
     1081 tggagcatga tgccgccggg cagggggcag agttgctgaa gaaaggcgga cgtgtgagcg
     1141 tacggtttaa ggtgccgggc gcactggtgc cgaatcagtt tgccctgggt atttactggc
     1201 agttgtcgtc cctgccggag ggagtgacgc tgtcagagga aggcaacgac atgctgatgt
     1261 ccttcttcct gcagacggat gcgacgaacc tgaacgcgat gtaccacaag aagcagaatg
     1321 cgaagctgga tacgttcggg gtctttgata acggatggca cacactggct tttgagtttg
     1381 ccggaaacaa cagcattcag gtgacgccgg tactggatga gaaacggggt acaccgttca
     1441 cactggtgaa atctccggca tcaggggcgg cggacaaact gcaactgaca gacatatcaa
     1501 aggcggcgac atatacgttg ctgattgaca gtgtgaaggt ggaagtgaac aacgcggatg
     1561 ccgcggcatg ataaaaaagg ccgccagcaa tgaaggaaag ggatgaactt tgtccactac
     1621 atgaatgctg gcggctggta aaacgaccat gatgacaccg aagtctgcgg tgtttttcta
     1681 caaaaataag taaatcatgt caactatcac ggtgagaaac catgacattt ctgaaccagt
     1741 taatgctgta cttctgtacg gtggtctgtg tgctgtatct tctttcgggt gggtacaggg
     1801 cagtgcgcga ttgctggcgc aggcagattg ataaaagggc cgcagagaaa atcagcgcca
     1861 gtcagtcagc cggaagcaaa cccgaagagc ccattactcc ttaataaccc ctttcaacga
     1921 gaaaatccca tgtcagaaat tacatccctg gtcactgcag aggcagtgaa ggaagtcctg
     1981 cgctctgaag aagtcctgag cgcactgaaa cagaaacttc gccataacct ggaagcgcgt
     2041 cttgatgcag aagtggatgc cattctggat gaactgctgg gcgcaccggc agctccggag
     2101 ccggaaggca tcgcgggtga ggggagtgct tcagatagcg gtgaccccac acctgacagc
     2161 gacatgatga tgtaagcatg cgcaagggac catcggtgtg tgccggtggt ctttatattg
     2221 ttgtgagctt ccgga
//
LOCUS       DAZUCC010000392         2230 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZUCC010000392 DAZUCC010000000
VERSION     DAZUCC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.394"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..362)
                     /locus_tag="ACHY09_005100"
     CDS             complement(<1..362)
                     /locus_tag="ACHY09_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS3887917.1"
                     /translation="MDISTLLLDFRITSHCNLGCDLCFRTPGIQDSSLASARNVIERM
                     YKMGFRRIGFTGGEPTSRSDFIDLIEYAKQLGFMTYLSTVGHRFIMDHARLNPVLDWV
                     GIPIDGIDSLVNSEIRSD"
     gene            847..1098
                     /locus_tag="ACHY09_005101"
     CDS             847..1098
                     /locus_tag="ACHY09_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS3887918.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            1095..1382
                     /locus_tag="ACHY09_005102"
     CDS             1095..1382
                     /locus_tag="ACHY09_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3887919.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHILSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            1550..1954
                     /locus_tag="ACHY09_005103"
     CDS             1550..1954
                     /locus_tag="ACHY09_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFS3887920.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHSWDDYVDNFFR"
BASE COUNT          663 a          440 c          505 g          622 t
ORIGIN      
        1 ttatcactgc gaatttctga attgacaaga ctgtcaattc catcaattgg tattcctacc
       61 cagtctaaca ccggatttaa ccgagcatga tccataataa atcgatgccc gaccgtggag
      121 agatatgtca taaaacccag ttgcttagcg tattcaatca gatcaatgaa atcactccgg
      181 gatgtaggtt cccctccggt aaaaccgata cgacggaatc ccattttata catccgctca
      241 attacattac gagcactagc aagagaagag tcctgaattc caggtgtacg aaaacacaga
      301 tcacatccta ggttacaatg tgaagttatt ctgaaatcta aaagaagggt gctaatatcc
      361 ataacaagtc ctctgactat gatacatcaa taaccattaa aacataatgg ttattgcaga
      421 ccatgacaaa gcgcaacctt cggcttacca gggatataat atctgccagt aatcagagca
      481 gtgggttgcg caaggaactc atgaatgatg agttccggta atcgcattac tggaagccca
      541 tgatcggagg gacgctatca gtcacaccag tgaaaattgt gcacagaagc attttcatca
      601 ctgacaggcc gtatgcctca caagacggga gataactgga gaatatttag ttcttctacc
      661 ggcttacgcc gattttactc ctgtgttata tgtaaccatt gtgtgccacc ttccatgcgc
      721 tggtgcggca tcagagtaaa aaatcagcat taaatttatt tgcaaatgta tgagtaatac
      781 agacttgtta acgggtcctt tcagatgtat ctttaagact tattaaacag tcttaaaagg
      841 tgcattatgc ctaacatcat tctgtctgac atcagtgcaa gtgtcagtga actgaaaaaa
      901 aatccgatgg cgacagtcgg tgctggtgat ggttatccgg tcgccattct gaatcgcaat
      961 cagccggcat tctactgtgt tcctgctgaa ctgtatgaaa ggatgcttga tgctctggat
     1021 gaccaggagc tggtgaaact ggttactgaa cgtagcagtc aacctctgca tgatgttgac
     1081 ctggacagtt attcatgact tatacgataa aattcaggga tgatgcgctt aaagaatggc
     1141 tgaaactgga taagaccatt caacaacagt ttgtgaaaaa actaaaaaaa tgtagcaaaa
     1201 atcctcatat actgtctgca aaactacgtg ggctaaagga ctgctacaaa attaaacttc
     1261 gtgcatcggg atttcgtctg gtttatcagg tgattgatga tatgttaatt attgcggttg
     1321 ttgcggtagg taaacgagaa cgtagtaacg tttattatct cgccagtgag agaatgagat
     1381 agtaacataa atagcctttc ccgcagataa ctctcagaat tgttattctc tctgtggcct
     1441 cgcctgaggc ggaaaaagaa aagttttgtt gagagaatga ttttacctgc cgggatgagt
     1501 ctggggttat ttatattacc agacaaattg aaacggaggg acatgtttga tgacaatact
     1561 tgtgacggca caggctcaac tcatagttgg tgaggctatg gcgataaaaa gtttagctcc
     1621 tgatggaatg ttggccgctg tatttgaaga cgacgggaat accggatatt tctatgcact
     1681 tgatgagtcc gttgaaggta accctgtcag ggatgctgtg catatatata acgttgaaga
     1741 catttcagat gctcacatcc cctctgatgt gaagattggc tggtctgagg attgcctgaa
     1801 gtgtgttctg ctgataaacg gatatccaca tggggtattc gattttgagg ggaagaacgg
     1861 ctactgtcga agtggattcc cgccaccggt taatcatgaa tggtctgttc aggggcattc
     1921 atgggatgat tacgtcgata atttcttccg gtgattgaac cgaaaaatta cctgacggac
     1981 aagtctgggg ccgaatcgtg atgattcatc atggccgttt atagttcagc agtcttgttc
     2041 ttttgctgtc agtgggatac caggaaaata accgattgtt accagacaga ataaccatca
     2101 taagacgtca caagacggtg acgcttacat agttccgggg agttaaacaa tataccctga
     2161 ctttgtaatc atttgaatta tatccgtaac gtgtaacttc ctgccgtaac accggattta
     2221 agatattcaa
//
LOCUS       DAZUCC010000393         2188 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZUCC010000393 DAZUCC010000000
VERSION     DAZUCC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.395"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2027
                     /locus_tag="ACHY09_005104"
     CDS             <1..2027
                     /locus_tag="ACHY09_005104"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFS3887921.1"
                     /translation="SVEPDIADRANSWLKSYGLDYKLEQAPLNNEIDNALDAYFSKNG
                     GAGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKEWNFKNIN
                     GYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWYVSKNNLGAGQK
                     VGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLNNDIYQNE
                     KGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRKIRNFLRL
                     KAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKIGLTTDFT
                     GKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGSAGLLVAA
                     MNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMGDGSSNIL
                     NKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDKGYAAVII
                     QSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIPHNAKQAV
                     KFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTEDEYFEGT
                     IDPDSGEDWNQTRPVDTRPTLEDFKKTVGDYLAWEVSKLLQGRDASEKKQ"
BASE COUNT          675 a          418 c          531 g          564 t
ORIGIN      
        1 aatctgttga accagacatt gccgaccggg ctaacagctg gctcaaatct tatggacttg
       61 attacaaact ggagcaggca ccacttaaca acgagatcga taacgcactg gatgcttatt
      121 tttcgaaaaa tggcggcgca ggcggtaatc gtcccgacgt aaaactcctt gttcaggaca
      181 aatacggcaa gcagtatccc gttctcatcg agtataaagg ctacaaggac aggcttatca
      241 gacttggtag caacggtatt attgagaaca aagatgccag aaaagaatgg aattttaaaa
      301 atattaatgg atacgcggtg aatggcgcag tccattatgc caacgcactt cttcagttca
      361 ccaattaccc tgatattatt gctattggta tgaccggctg gcgcgatgat gatacaggcg
      421 aactgcatca tgaaatcggc gtgtggtacg tatcaaagaa taatttagga gccgggcaga
      481 aggttggtga attcaccgac ttatcattcc ttgctgacaa aaatgttgat ggatttctca
      541 acaagataaa gctgcttaat cttcctcccg aagaactgga aaaaatcaag gccagcaaag
      601 aggaagagat agacacgcga ctttcccgtc tgaataacga tatttaccag aatgaaaagg
      661 ggcttggtga aagcgatcgt gtttaccttg ttgtggctac cgttattgca acgttgggga
      721 ttccgggaaa gttagcaccg ctggataaaa aagaactgac ctcttcaacg gaggaggact
      781 tgcgtgatgg tgatattatt ttcaggaaaa taaggaattt tttaaggctg aaagctgttc
      841 ctgaaacaaa aagagaaatg attttgcgct cgttgcaaaa tacgctgtgg actgaaaaca
      901 ttaacaaacc agtcaacggt gaaagccagt taaagcgcgt gtttgttaaa gttgttgatg
      961 atcttgggga gtattacaaa atcggactga ccaccgattt tacgggcaaa ctgtttaatg
     1021 agatgtatcg ctggcttgga tttacgcagg acaaactcaa tgacgtcgtt ctgacaccac
     1081 cttatgttgc cacattgctg gcacgactcg ccagagtaaa caaagattcc tatgtgtggg
     1141 attttgctac aggttctgcg ggattgcttg tggctgcaat gaatgaaatg ctcattgacg
     1201 ccagagaaaa tattcactca ccgaatgaac tacaacttaa ggaagcgcaa atcaaggctg
     1261 aacagcttct tggccttgag gtgttatcca gtatttatat gctggccatc ctgaatatga
     1321 ttttgatggg ggatggtagc tcgaatatcc tcaataaaga ctcgctggcc gattttgacg
     1381 gcaagtatgg atttgggaaa acaggagaaa aattccctgc ggacgcattt attctcaatc
     1441 ctccgtattc agctaaaggt aacggcatga ttttcgtgca gaaggcgctg tcgatgatgg
     1501 ataaaggcta tgctgcagtg attatccaga gttcagccgg cacaggaaag gctacggagt
     1561 acaacaaaaa aatactcaag gaaaatacct tgctggcaag catcaaaatg cctgcggatc
     1621 tgtttattgg taaatcaagc gttcagacgt acatttacgt ttttcaggta aaaattccgc
     1681 ataacgcgaa acaggctgtt aagtttatcg acttttccaa tgacggctat gctcgctcta
     1741 atcgcaaaaa ggcaagaaac aatcttgttg atgccgatcg tgcaaaagag cgttatcagg
     1801 aggttgtgga tctggttcac ttcgggaaag gttgccttaa tatctttacg gaggatgaat
     1861 attttgaggg aacaatcgat cctgatagtg gtgaggactg gaatcagacc aggccagtag
     1921 ataccagacc gacactggag gattttaaaa agacggttgg cgactatctt gcgtgggagg
     1981 tgtcaaaact tcttcagggg cgtgatgcgt cagaaaaaaa gcagtaaact cccgtcttgc
     2041 agagcttgag cgggagttta ttaaatctgg tggtaaatgg gaagaataca caatcgaaag
     2101 attgttcagt attcagacac ctaaaaagaa atttaatgcc aataaattaa catttaatgg
     2161 caactatcct tatgttgcca gaggaagt
//
LOCUS       DAZUCC010000394         2160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZUCC010000394 DAZUCC010000000
VERSION     DAZUCC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.396"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..259
                     /locus_tag="ACHY09_005105"
     CDS             80..259
                     /locus_tag="ACHY09_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFS3887922.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            345..845
                     /locus_tag="ACHY09_005106"
     CDS             345..845
                     /locus_tag="ACHY09_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS3887923.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETYGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            891..1397
                     /locus_tag="ACHY09_005107"
     CDS             891..1397
                     /locus_tag="ACHY09_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS3887924.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNDEKHMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1456..2094)
                     /gene="ompW"
                     /locus_tag="ACHY09_005108"
     CDS             complement(1456..2094)
                     /gene="ompW"
                     /locus_tag="ACHY09_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFS3887925.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          685 a          481 c          471 g          523 t
ORIGIN      
        1 cattagcgac taattttaat gagtgtcgac acacaacacc catattaatg aaacaatgca
       61 acgcaacggg agaaataaca tggccgaaca tcgtggtggt tcaggaaatt tcgccgaaga
      121 ccgtgaaaag gcatccgacg ctggccgtaa aggcggtcag catagcggcg gtaattttaa
      181 aaatgatccg caacgcgcat ctgaagcggg taaaaaaggc ggtcaacaaa gcggtggtaa
      241 taaatcaggc aaatcctgat tcaccgttaa ttattaatat cagctgataa agataattgc
      301 atctgcgggc cgtatggctc gcagtgattt cactggagaa aaatatgaat atgaagacca
      361 ttgaagatgt atttattcac ctgctttcag atacctacag cgcagaaaaa caattaaccc
      421 gggcactggc aaaactcgca agagcaacat caaacgaaaa attaagtcag gcttttcatg
      481 cgcacctcga ggaaacatat ggacagattg aacgtattga tcaagttgtg gaatcagaat
      541 cgaatctgaa aattaagcgc atgaaatgtg tggcaatgga aggtcttatt gaagaagcta
      601 atgaggtcat cgaaagtacc gagaaaaacg aagtgcgtga tgccgcactg attgccgcag
      661 cacagaaagt cgagcattat gagattgcca gttacgggac attagcgacg ctggctgaac
      721 aattaggtta tcgtaaagca gcgaagcttc tgaaagagac cctggaagaa gaaaaggcca
      781 ccgacatcaa attgactgat ctggccctta ataacgtaaa taagaaagcc gaaaataaag
      841 cctgaaatat gaactttaac ttttagtcat tttataaaga ggacattttc atgaatcgta
      901 ttgaacatta tcatgactgg ttacgtgacg cccacgcaat ggaaaagcaa gccgaatcaa
      961 tgcttgaatc catggccagt cgtatagata attatcctga actacgcgct cgtattgaac
     1021 aacatcttag tgaaaccaaa aaccagattg ttcaactgga aactattctt gatcgtaatg
     1081 acatttcacg ttcagtcatt aaagattcca tgagtaaaat ggctgcactt gggcagtcaa
     1141 tcggtggtat attcccttct gatgaaatag tcaaaggctc tattagcgga tatgtcttcg
     1201 agcaatttga aatcgcctgt tatacctcac tcttagcagc agcaaaaaat gccggtgata
     1261 cagcctcaat tccgattatc gaagcgattt taaatgatga aaagcacatg gccgactggc
     1321 taattcagca tattccgcaa acaactgaga aattcttaat tcgctctgaa actgatggcg
     1381 tagaagcgaa gaaataatac cctttatacc atgtccttat tgaccccgta attacggggt
     1441 catttttgtg cggaattaaa aacgatatcc tgctgagaac ataaacaccc acggatcgag
     1501 gcgtacgcta tcgtgttgct gtgcaccgcc cagtttataa ttggcagtgg tatcaatatc
     1561 catgtaccag actgacatgt taaccaacca gtcacggtta atcagataat caacccccac
     1621 ctgcccggca gctccccagg aatctttcag actgagatcg gaaagccccg cctctttgcc
     1681 atgatcgtta aatccattat caaagaaggt ggtgtagtta atacctgccc caacgtaagg
     1741 acggaatttg ctgctggcat caccaaaata ccactgcgcc atcagtgttg gcggcagatg
     1801 atgaacggtt gcaatatcgc cggtcgcccg ggtgccgatt ttatggcgga acggcgtcgc
     1861 tgccagcaat tccacaccaa tgttatcggt cgccatataa gtaaacgtca ggcccagttg
     1921 cgtattattg gtcacgctga atccacccag acttcctaac gtaccaccag caccttctgt
     1981 tggacgtacg gttgcagaac ctgcacgcat aaaaaattcg cctgcttcat gcgcaaaggc
     2041 actgccagag agaagagttg ttactgccaa agccgccact gttaactttt tcatatccgc
     2101 tccgtcgtta tggttataaa aacgtgacga atatacctac attggagtaa caaatgatcc
//
LOCUS       DAZUCC010000395         2144 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZUCC010000395 DAZUCC010000000
VERSION     DAZUCC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.397"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..1304
                     /locus_tag="ACHY09_005109"
     CDS             99..1304
                     /locus_tag="ACHY09_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3887926.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGVAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFKKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLACALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFKTKLWEIPAEVMKMKRPHIVP
                     LSEQVIALFKQLEPITKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIETQLAHVDKNAIRGTYNHALYLDGRREMMQWYADYIDSLSSH
                     ESQCQGPLY"
     gene            1712..1978
                     /locus_tag="ACHY09_005110"
                     /pseudo
     CDS             1712..1978
                     /locus_tag="ACHY09_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          662 a          461 c          443 g          578 t
ORIGIN      
        1 acatctttgt tatacaaggg gtgcattggg ggtatcaggt taattactgg cagaaatccc
       61 cctcaggaac atgaattcat taaaagccgg aggatgtcat ggcattaacc gatattaaag
      121 tgaaaaccgc aaaaccaaaa gaaaagccct ataaacttgc tgatggcggc ggtatgtatc
      181 tactgattaa cactaatggt tctaaatact ggcgtatgaa gtaccgcttt gctggtaaag
      241 aaaagatgct ttctattggc gtataccccg atgtaacgct agccgacgct cgtgaaaaac
      301 gcagcgaagc cagaaaaatc ctcgctgctg gaggcgatcc aggggtggca aaaaaagaag
      361 aaaaaatcgc gcagcaaatg agtttgaaaa atacgttcga ggccattgct cgcgaatggc
      421 atcagtcaaa agccgatcgg tggtcattac gctatcgtga tgaaatcatt gataccttta
      481 aaaaagacat ttttccctac attggcaaac gccctatcgc cgaaatcaaa ccaatggagt
      541 tactggaagc actgcgtaaa atggaaaaac gtggagctct ggagaaaatg cgcaaagtcc
      601 gccagcgctg tggtgaggtg ttccgctatg caattgttac tggtcgggca gactacaacc
      661 ctgctcccga tcttgcctgt gctttagcta caccgaaaaa agtacatttt ccctttctta
      721 ctgctaatga acttccccac ttcctcaacg atctggcggg ttataccgga agtatcatca
      781 ctaaaacagc tactcagatc attatgttaa ccggtgtacg aacacaagaa ttacgttttg
      841 cacgctggga ggatatcgat tttaagacaa aattatggga gattccggca gaagtaatga
      901 agatgaagcg acctcatatt gtgccgctct ctgagcaggt cattgcccta tttaagcaac
      961 ttgaacccat cacaaaacat catcctctgg tctttatcgg taggaacgat cctcgcaagc
     1021 caattagtaa ggaaagcatt aaccaagtca ttgaattact gggatataag ggaagactca
     1081 cagggcacgg tttcagacac accatgagta ccattctaca cgaacagggc tttaattccg
     1141 cttggattga aacgcagtta gctcatgtag ataaaaacgc tatcaggggg acctataatc
     1201 atgccctgta tctagatggt cgccgtgaaa tgatgcaatg gtacgctgat tacattgatt
     1261 cgctttccag ccacgagagt cagtgtcaag gccccttgta ctgatatttt ctctcaattc
     1321 acaaaattcc tccggcctcc aggtctggag gaattttgtt ttcatgtttt atttttagat
     1381 attttttgac aaaatattta tattgcatct gcaaacaatc tcagaacatc acaccgctat
     1441 agaccgctaa caattttgat gcctgtaatt tcaatcatca tcctgtcgtt atttcagcac
     1501 catcacacca aaaatcaact aacatgttgt cttagccgac tattcacaat gcattggaat
     1561 gcagataaag ataaattttt atccacatga agaattactg tcgatgatat tgtaaacaat
     1621 attcatcccg ggcaataaca agacttgata cacaacatca cgacttttat gcttttcaga
     1681 ttgtgtccca tccgttttac aggctcagaa aatgaacgac agattacgct ttgatgtgaa
     1741 cgataaccaa ggccgtttcg tatttccgaa taccaggttt ggcccattac tggaggagtt
     1801 tgaagaagtg catgacgcat acggcactga tgaaatcagc gagacgagtt atatcaacaa
     1861 actcaggtag cttgcccaac gggagcctga ctttattgat atacacgctc atctggcata
     1921 tgcatttctg gagcagccaa ggtgggcaat atttttattt atttctgtga aaagcctgct
     1981 aaagatccca tgcttctata ttaatgactg gaacaatact aggaagcaca ttaagaaaaa
     2041 ataaaggaag ccgatggcta ccacgtttat cgtttagaag cgtgagctct tcattattaa
     2101 gccggacact tacaaaatga agttgcttct gttaaaattt cgtt
//
LOCUS       DAZUCC010000396         2120 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZUCC010000396 DAZUCC010000000
VERSION     DAZUCC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.398"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(727..909)
                     /locus_tag="ACHY09_005111"
     CDS             complement(727..909)
                     /locus_tag="ACHY09_005111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887927.1"
                     /translation="MTMLVYGVKTATEMLILYVMLILFPEHPVDVNNFQIFGSESLDI
                     SIYATVDVFCGAWQCD"
     gene            complement(985..1656)
                     /locus_tag="ACHY09_005112"
     CDS             complement(985..1656)
                     /locus_tag="ACHY09_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFS3887928.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGIGRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNEMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQA"
     gene            complement(1698..>2120)
                     /locus_tag="ACHY09_005113"
     CDS             complement(1698..>2120)
                     /locus_tag="ACHY09_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3887929.1"
                     /translation="DPGAQGPKGETGATGPAGPRGAQGPKGEKGDPGPRGERGETGPM
                     GPPGASGDDGKSRVMGFRLGNPVTYELQEGGHGWSVNLADGSVITGLSGNTQGGVSRV
                     TTIYYRPLQMTFDGSHWRTVGVGEYVSSGAPGREYFSL"
BASE COUNT          499 a          649 c          424 g          548 t
ORIGIN      
        1 gcccttaccc tgaagagcgg agttttacgg tgcgctaagt aaaacgtaag tgctgtccct
       61 agtcgtggat tgctgtagct gagagtcgga aacgtgctca aaacgtgcct gacagcgaga
      121 acgctaacaa attaaaaact attgcctgaa aaaaatcgcg ttaaaaagtg tatgagtgca
      181 tttccagcga gcacaaaagc cacattcagt gaattgcctc agcagagccc cagaaataca
      241 ctcaacccga taaaaatact tgccgaaaat tccggtgata tttcaaccct caagggggat
      301 tttttatgca atcaccgcaa caaaaactgg acatactgaa atacacgatg ccctgttacc
      361 agtgcgtttc tggcatcaat gtagtttgta gggatgatta cagtcaggca tgtgccagaa
      421 acacgctcaa ttctcaatct attccttgca accgaccaac tccgtgacct tgatatccac
      481 cgcagtctga agcatacaac cgggcccgtg caaaatacgg acttccggaa atctgaaaaa
      541 ttactcttat cagagaagag cacagaaaca cgatcggcat cactgtttct gcaaacttcc
      601 ctgtggcagt ctttatttgc catataacat agcactgaat gtcttcatta atgtttccat
      661 tatagttaat acttccttgt accggggggt ggtgataccc ccgctttttt cgttatccct
      721 gttcccttaa tcacactgcc aggcgccaca aaacacatca actgtggcat atatggaaat
      781 atccaaactc tcacttccga aaatctgaaa attattcaca tcaacaggat gctccggaaa
      841 caagatcagc atcacgtaca gaattaacat ttccgtggca gtctttacgc cgtatacaag
      901 catggtcatt tatttagatc cttctaaatg tttccgctga tacttttctt tggtcagggg
      961 ctgtttatca gccccccttt tttattacgc ctgtgcccag ccaactctgt atgccagtat
     1021 ctcatccgca ctggtcagct tttccagctc cttcttcatg gtgcgctggc gaacgtggat
     1081 ttccatccct ttggtgaaca tcgcctgctc tgccgcttca ctcagcgcta taagctcttc
     1141 tgctgtcacc tccacatcat tattttccgc atccgtccag aaaaacgcct ccggcagttt
     1201 ccccgctttc gctgccgcca ccgacggctc aagacgcgtc tgcgttgact tcccgtagtc
     1261 ccatttacgc ccattgtgct caaacgtgta gttcgccgct tccatctcat tacgccaggc
     1321 gttaatttca tcgcccttca tcccgcgcgc tttctccgcg gtcagcaggt ccgtgatttt
     1381 ctccccgtca aaaccccagc gccccgagat gtcagctttg cggttttctt ccgtatccgc
     1441 cacctccacc acactctggc ccactggcca cagaactgac gccaccctgc cgatgccggt
     1501 aatgactccg gcactgttat aaaccacctt cagcgtctct gctgaaaata acgactgaca
     1561 ctcataccag tcctgaccat cctctgattt caggtacatc gcacccggca catccggatt
     1621 ttccggcgtg tattgtctga aattctttat gtgcatctgt aagtcctccc ggttacatgt
     1681 ctgaaagatg atgcgcttta taatgaaaaa tactcacgcc ccggcgctcc tgacgacaca
     1741 tactcaccaa caccaacggt gcgccagtga gagccatcaa acgtcatctg cagcggcctg
     1801 tagtaaatcg ttgtaacacg gctgacgccg ccctgagtgt tccccgaaag ccctgtaatg
     1861 actgaaccat cagccagatt aactgaccag ccatggcccc cctcctgcaa ctcatacgtt
     1921 accggattcc ccagcctgaa gcccatgaca cgactcttcc cgtcatctcc tgatgccccc
     1981 ggcggcccca tcggcccggt ttctccacgc tcacctctgg gaccggggtc tcctttctct
     2041 cctttcggtc cctgcgctcc ccgcggacca gcggggcctg ttgctcccgt ttcacctttc
     2101 ggaccctgtg ctccggggtc
//
LOCUS       DAZUCC010000397         2117 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZUCC010000397 DAZUCC010000000
VERSION     DAZUCC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.399"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            175..384
                     /locus_tag="ACHY09_005114"
     CDS             175..384
                     /locus_tag="ACHY09_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS3887930.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            complement(404..637)
                     /locus_tag="ACHY09_005115"
     CDS             complement(404..637)
                     /locus_tag="ACHY09_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887931.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(726..1349)
                     /locus_tag="ACHY09_005116"
     CDS             complement(726..1349)
                     /locus_tag="ACHY09_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3887932.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            complement(1438..1947)
                     /locus_tag="ACHY09_005117"
     CDS             complement(1438..1947)
                     /locus_tag="ACHY09_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887933.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGINGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWPVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
BASE COUNT          578 a          564 c          476 g          499 t
ORIGIN      
        1 tatcagaaaa agagaaggac atgaaagagg agagcaataa aagacacgga aaataaagaa
       61 gagagagaaa atatttttaa aaacagaaaa ttaacaacgc acatcaggaa acgtcgtctc
      121 ctgattcagt gataccacat gaaaatttag tgaacggtta ccgttctgaa gtctatgtca
      181 gaagatattc cattaccaaa aatcaatcag cgatacagag acgatcatgg cgcgcttgtc
      241 accgtgacca gtgttgagga aacccgggtg gtgtttatgc gggacggcta tccgcatccc
      301 tgcatgcggc cgatgtacaa cttcctggga aaattcaaac ccgaaccccg gaaagaaccc
      361 cctgccggca atcacactgc ctgatggcag cataatccac ttattaattc tcatccccgg
      421 agacaagccc agaaaaccac tccgggtgtt cactgtatgc cgtgttcagc gcatcccaag
      481 aaacgttgat ataaaagctg tcctgcaatt tgcgggtaac aatttctgcc acccggttac
      541 ggttatccgc ctgtccggca taacctctct ctgccagcca ctcattcagt tcatgttccg
      601 gagacagatt gataatatcg taatcggacc ttgccattcc cgtttcctta gcacatgttt
      661 tcaggctcgg ggataaagaa tgtcgtactg ctgctgccag agcaatattc cccctgatgg
      721 tcgaatcaga gatggtaggc cggtactttg cgggttgttc ccgtcagatt atcatgcacc
      781 tccgcggtcg ttgtcaccac cggcattccg ctctttctca gcctcaggac atcagccgca
      841 atacgctgac taccgaacca gaacaggccg tccagtgcag tcaccagcaa ccccgcctcc
      901 agcgcatgct tcagccgttc acggggcgct ttcacttccc gggcaatctg ctggtatggc
      961 gatgatgtgt tttcattccc aatcacccgg cgaatacgat gagacagatg ataccggtat
     1021 gtatccggta caccggaaag gctggccttc aggctgtaca cgcagccaaa tcgtttatca
     1081 ttgaacacca catttttctg gctgatgccc cattcttcac gcagcgcggc aatcagttgt
     1141 ggtgtccggg taagcaacaa gcgaaaaggc agttcaaaac tggtgacgta atccacattc
     1201 agcagtgcaa tacgcagccg ttcttccggc ccggcctctg tctgccggca ctcctccaga
     1261 acatcctgcc actgctggtg aaggcgtgga agttcattca gttcagtaaa actgtattta
     1321 tccgcaagat agtcaattcg tgtacgcata ctgaagagta ttccgtataa agattcagct
     1381 ggcaaaactt tatcagtctg taaaaactaa cggaagagtc gatatttctc ccgtccatca
     1441 ccggatgatt gttgcaatac cccgtggcat cagagactga acagccgttt ttaacgccac
     1501 gtattgccct gatgaaccag gccgggcaac ccgaaaacat ctttccaccc ggcattgtcc
     1561 gccagcactt atcaccggcc aggtctgttg cagtaaatcc accagttgcg cacattcttc
     1621 atcagccatg acgccgactc gcggatggca aatactcgtc tggctgagca gcaacagaca
     1681 tcgtatctgt gctccttcat gttgttcccg caaccagcgt aataccaccg gcgctgatgg
     1741 gctgaccgtg tgattatgct ccgtaatacg ttcgtgtacc cgtcggtgaa aggaactaca
     1801 aaatgtctga atctgttgac cattgattcc ttctgccgaa tagagcgtga agtgaaatgc
     1861 caacagattt ggccagtggt ccaccgcctg ctgaacaaaa cgccggattt cccccggctc
     1921 tgaaattaag gcttcggtta tttgcacgaa atgttccttt tcaacccctg ttgaattttg
     1981 tcaagccggt gcaggcacag taaagcgcgc atcaacacct gaaaggtacg atacaaacac
     2041 aagttacaaa caataagaac ggaaggacaa ttaatcagtg aggaataata actaaattaa
     2101 ccaaaagaat gttactg
//
LOCUS       DAZUCC010000398         2109 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZUCC010000398 DAZUCC010000000
VERSION     DAZUCC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.400"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..449)
                     /locus_tag="ACHY09_005118"
     CDS             complement(<1..449)
                     /locus_tag="ACHY09_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3887934.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMPEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDVVRSIQTQFPRQVGQLSVMYRCLPDCHHDEAVL"
     gene            complement(449..1138)
                     /gene="tssL"
                     /locus_tag="ACHY09_005119"
     CDS             complement(449..1138)
                     /gene="tssL"
                     /locus_tag="ACHY09_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFS3887935.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSGEGGEPDVDEGQAAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSPDPAVLTCFHRVTALGFQGVYGLKAVSQSQRDE
                     VMKALSERVSLPDGGLSLVVYRMEKHRWRLIRSVWFWTVLAVILTGVIWWGGYLWLQS
                     LLSSQIPELHG"
     gene            complement(1135..>2109)
                     /gene="tssK"
                     /locus_tag="ACHY09_005120"
     CDS             complement(1135..>2109)
                     /gene="tssK"
                     /locus_tag="ACHY09_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3887936.1"
                     /translation="VRYREEWVTLQDAFGSEEEPIAVARFNFTIRFAHENNDSWKVCP
                     VARLIRDGQNAWRQDPSFIPPVALFGASPVLRERLVLLNRQLRSRRQRLMAMRRESNE
                     RLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAGSMLTFSLDH
                     DPDAIPGYDHKDPAGAFLPLFDLITGLLEACLPSRVIALEMSRPDGQTWKASLHDIRL
                     REEADLWLSVRSDTPAWQVAEKFPALCLAGSPDNVSEIYGAALRGIPLIPVSRVPAAL
                     PVRMENQYFALDMESPAAHEMLEQGVCMFYVPELLGALELELFAVLRS"
BASE COUNT          539 a          629 c          537 g          404 t
ORIGIN      
        1 agaaccgctt catcatgatg acagtctggc aggcagcggt acatcacaga gagctgaccg
       61 acctggcgcg ggaactgcgt ctgtatgctg cggacaacgt cagttaagcg actgacgtca
      121 ccgacccgca gccaccatcc ccgggctgtt ttacgcagcg gaccgtcctg aaacaacgct
      181 tccggcatgt ctccacatac cagtacaaca gggccccggg tgtcggataa tgccggttca
      241 gcatccgtca gggtaacgtc agatgtgagc ttttgctttt cactccggct gtttttaaaa
      301 acactccagc ctgccagaag aaggatcagc agagtagcca gaccgttaaa aacgttgcct
      361 gccgggatga aataccacag caaccacagc aacgtggaaa cggccaccag ggcatgcagg
      421 gctaccggaa tgatgacaga tccacgcatc agccatgcaa ctccggtatt tgtgatgaca
      481 aaagagactg aagccagagg taaccccccc accagataac gccggtaaga atgacggcga
      541 gcactgtcca gaaccagact gagcgtatca gacgccagcg atgtttctcc atccggtaaa
      601 caaccaggga cagaccacca tcaggcagag aaacacgttc agaaagggct ttcatgacct
      661 catcacgctg tgactggctg actgctttca ggccgtacac cccctgaaaa cccagtgctg
      721 tcacccggtg aaagcaggtt aaaacggccg ggtccggtga aggctgacgc agaacctctg
      781 caatgcgatc ccacagtgct tcaccagccc gaagagaacc aaaaaagcgg gcctgaagcg
      841 gcactttacg ccaggccgcc tggccttcgt caacgtccgg ctccccgcct tccccgcttt
      901 tttcaggctt acggctcatc accgtttcgt ccaggagggc acattgtgca taggtaatat
      961 gctccacact ggcatcgtca taaccggcac gctccagcgc ttcccgcaca ctttcgacct
     1021 gtttaacgca tttgttgtaa agcgcagtgc catcgggcgt gagtgcacct tttttcagca
     1081 tggtgacggt aagccatgtc tccatcatca gctggtcgat atcaatatcc tgtctcatga
     1141 gcgcaatacc gcaaacagtt caagttccag ggcaccaagt aactcaggca catagaacat
     1201 acacactccc tgctccagca tttcatgtgc agcgggactt tccatatcaa gtgcgaagta
     1261 ctggttttcc atacgtacag gcaacgcagc cggaaccctg ctcaccggga tcaaagggat
     1321 ccccctgagt gccgcgccat agatctcact gacattatca ggtgaacccg ccagacagag
     1381 tgccggaaat ttctcagcaa cctgccaggc cggagtatcc gagcgcacag acagccagag
     1441 gtccgcctct tcgcgcaggc ggatatcgtg aaggctggcc ttccaggtct gtccatccgg
     1501 gcgggacatt tccagagcaa tgacccggga tggcagacag gcctccagca gacccgttat
     1561 caggtcgaac agcggaagaa acgcaccggc cggatccttg tggtcataac ccggtatggc
     1621 gtcaggatca tgatccaggg aaaaagtcag catactgcct gccagacgag cgagctccgc
     1681 ccacacctgt tcaggtggac gggaaggaaa acgttcgtat tccgtcagta cactggcgtg
     1741 ggagttcagc gcattcagca accagaaaag ggaaacatca gcgacagcaa aatcggcgag
     1801 tctttcgtta ctttcacgac gcatcgccat cagacgctgg cgtcgggagc gtaactgacg
     1861 attgagcaac accagacgtt cgcgcagaac cggacttgcc ccaaataacg ccaccggagg
     1921 aataaaagac ggatcctgtc gccacgcatt ctgaccatct ctgatcaatc ttgctaccgg
     1981 acagactttc catgaatcat tgttttcatg ggcaaaccgg atggtgaaat taaaccgggc
     2041 aacggctatg ggctcttctt cagagccaaa agcatcctgc agcgtgaccc actcttcccg
     2101 atagcgaac
//
LOCUS       DAZUCC010000399         2082 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZUCC010000399 DAZUCC010000000
VERSION     DAZUCC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.401"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            70..146
                     /locus_tag="ACHY09_005121"
     tRNA            70..146
                     /locus_tag="ACHY09_005121"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:104..106,aa:Arg,seq:tct)
     gene            238..1197
                     /gene="stxA2b"
                     /locus_tag="ACHY09_005122"
     CDS             238..1197
                     /gene="stxA2b"
                     /locus_tag="ACHY09_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFS3887937.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1210..1473
                     /gene="stxB2b"
                     /locus_tag="ACHY09_005123"
     CDS             1210..1473
                     /gene="stxB2b"
                     /locus_tag="ACHY09_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFS3887938.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            1979..>2082
                     /locus_tag="ACHY09_005124"
     CDS             1979..>2082
                     /locus_tag="ACHY09_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS3887939.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSA"
BASE COUNT          539 a          454 c          517 g          572 t
ORIGIN      
        1 agagcgccag ccttcgaagc tggttgcgcg gggttcgagt cctcgatggc ggtccattat
       61 ctgtacactg cgttgttagc tcagccggac agagcaattg ccttctaagc aatcggtcac
      121 tggttcgaat ccagtacaac gcgccatact tattttttct ggctcgcttt tgcgggcctt
      181 ttttatatct gcgccgggtc tggtgctgat tacttcagcc aaaaggaata cctgtatatg
      241 aagtgtatat tgttaaaatg ggtactgtgc ctgttactgg gcttttcttc ggtatcctat
      301 tcccgggaat ttacgataga cttttcgacc caacaaagtt atgtctcttc gttaaatagt
      361 atacggacag aaatatcgac cccacttgaa catatatctc aggggaccac atcggtgtct
      421 gttattaacc acaccccacc gggcagttat tttgctgtgg atatacgagg gcttgatgtc
      481 tatcaggcgc gttttgacca tcttcgtctg attattgagc aaaataattt atatgtggcc
      541 ggattcgtta atacggcaac aaatactttc tacaggtttt cagattttgc acatatatca
      601 gtgcccggtg tgacaactgt ttccatgaca acggacagca gttataccac tctgcagcgt
      661 gtcgcagcgc tggaacgttc cggaatgcaa atcagtcgtc actcactggt ttcatcatat
      721 ctggcgttaa tggagtttag tggaaatgcc atgaccagag atgcatccag agcagttctg
      781 cgttttgtca ctgtcacagc agaagcctta cggttcaggc aaatacagag agaatttcgt
      841 ctggcactgt ctgaaactgc tcctgtttat acgatgacac cggaagaagt ggacctcaca
      901 ctgaactggg ggagaatcag caatgtgctt ccggagtttc ggggagaggg tggtgtcaga
      961 gtggggcgaa tatcctttaa taatatatca gcgatactgg gcacagtggc ggttatactg
     1021 aattgccatc atcagggggc gcgttccgtt cgcgccgtga atgaagagat acaaccagaa
     1081 tgtcagataa ctggcgacag gccagttata aggataaaca atacgttatg ggaaagtaat
     1141 accgcagctg cttttctgaa tcgcagggct cactctttaa atacatccgg agaataacag
     1201 gagttaaata tgaagaagat atttgtagcg gctttatttg cttttgtttc tgttaatgca
     1261 atggcagctg attgtgcaaa aggtaaaatt gagttctcta agtataatga gaatgataca
     1321 ttcacagtaa aagtggccgg gaaagagtac tggactaacc gctggaatct gcaaccgcta
     1381 ctgcaaagcg cacagttaac aggaatgacg gtaacaatca aatcaaatac ctgtgcgtca
     1441 ggttcaggat ttgctgaagt gcagtttaat taatatcaga agcattgctg gtttcgtggt
     1501 gtgcagcaat gtagttacag tgtaatcaat gtcacaattc agtcagttga aggttgtctg
     1561 cccgactgag aatttgttaa aaaaatcctg catggtgaat ccccctgagc ggaggggcat
     1621 atcagcgtca caggtgtttc tgttttacct ctatcctttc tgtgcgggtt caggtgctga
     1681 tactgaactc accgggaggc acccggcacc atgcatgaac ggtacatagc gcatacacca
     1741 gcccctctcc ggaggggctt tcttgtgggc aaaaaaaagc ccgagtgggt tcgggcaata
     1801 gcatgagata tacattttta taatcgaatg gattttaacc aggattcata aggctgcgca
     1861 actgcgcggc ctttttcgta ttgcgggctg tagtcttcct ccagtcattg tcctgtaact
     1921 tccggacttc agcccgcccc ttatctgact cacaacatta tcccggccgg gaggattcat
     1981 ggcatttaaa cactatgacg tggtcagggc ggcgtcgccg tcagaccttg cggaacgaat
     2041 aactcaaaaa ctgaaggaag ggtggcagcc ttatggtagt gc
//
LOCUS       DAZUCC010000400         2072 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZUCC010000400 DAZUCC010000000
VERSION     DAZUCC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.402"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..238
                     /locus_tag="ACHY09_005125"
     CDS             137..238
                     /locus_tag="ACHY09_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887940.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            235..690
                     /gene="ybcN"
                     /locus_tag="ACHY09_005126"
     CDS             235..690
                     /gene="ybcN"
                     /locus_tag="ACHY09_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFS3887941.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            690..860
                     /locus_tag="ACHY09_005127"
     CDS             690..860
                     /locus_tag="ACHY09_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFS3887942.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            853..1143
                     /locus_tag="ACHY09_005128"
     CDS             853..1143
                     /locus_tag="ACHY09_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFS3887943.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            1140..1502
                     /gene="rusA"
                     /locus_tag="ACHY09_005129"
     CDS             1140..1502
                     /gene="rusA"
                     /locus_tag="ACHY09_005129"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFS3887944.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1499..1639
                     /locus_tag="ACHY09_005130"
     CDS             1499..1639
                     /locus_tag="ACHY09_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFS3887945.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1725..>2072
                     /gene="quuD"
                     /locus_tag="ACHY09_005131"
     CDS             1725..>2072
                     /gene="quuD"
                     /locus_tag="ACHY09_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFS3887946.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIR"
BASE COUNT          547 a          471 c          535 g          519 t
ORIGIN      
        1 gaaaagcttc cagagagtaa aagtgtttta agaaggagca attctatcga ttttgatttt
       61 gggaaatcaa cacggcataa ttatgtcacc ggagcctgaa caactccggt gacttctgcg
      121 ctaaacgggg acgtttatgc gcacatacaa tccaaactct cttctccctt cacagatgca
      181 gaaatgcacc tgcaattctt tgcatctagc gtttgacctc tgcggaggtg aagcgtgaac
      241 ctctcacaag acggcatcaa attacatcgc ggcaacttca ccgctatcgg tcggcagatc
      301 cagccttatc tggaggaggg caaatgcttt cgcatggtgc ttaaaccgtg gcgtgagaaa
      361 cgcagtcttt cccagaatgc actcagccac atgtggtaca gcgaaatcag tgaatacctc
      421 atcagcaggg gtaaaacgtt cgccactcca gcttgggtaa aagatgctct caaacacaca
      481 tatctcggtt atgaaaccaa agacctggtt gatgtcgtaa ccggtgatat caccactatc
      541 cagtcgttac gccatacctc cgatcttgat accggagaga tgtatgtctt cctgtgtaag
      601 gttgaagcct gggcggtgaa tattggctgc cacctgacta ttccgcagag ctgcgagttc
      661 cagctgctcc gcgacaagca ggaggcgtaa tggctacacc gcttattcgt gtcatgaacg
      721 gacacatcta cagagtatca aatcgtcgta agcgtaagcc tgagctgaag ccatccgaaa
      781 taccaacact gctcggatat accgctagcc tggttgataa aaaatggttg cgactggcag
      841 caaggaggaa tcatggctga tttgagaaaa gcagcgcgtg gtcgggaatg ccaggtaaga
      901 atccctggcg tatgtaatgg caattctgaa acgtctgtac tggcacatat ccggctggct
      961 ggattgtgcg gtaccggtat caaaccgcca gacctgattg ccaccattgc atgttctgcc
     1021 tgccacgacg aaatcgaccg ccgcacacat tttgtcgatg ctgcatatgc aaaagaatgc
     1081 gcgctggaag gtatggcgag aacacaggtt atctggctga aagagggggt tattaaggcg
     1141 tgaataccta cagtatcaca ttaccctggc ctccgagcaa taatcgctat taccgccata
     1201 atcgcgggcg cacgcacgtc agtgcagagg ggcaggcata ccgcgataac gtcgcccgaa
     1261 tcattaaaaa cgcaatgctg gatatcggcc tggctatgcc tgtgaaaatc cgcattgagt
     1321 gccacatgcc ggatcgccgt cgccgtgacc tggataatct gcaaaaagcc gcttttgacg
     1381 cactcaccaa agcaggtttc tggctggatg atgctcaggt cgttgattac cgcgttgtga
     1441 agatgcctgt taccaaaggt ggcaggctgg aactgaccat caccgaaatg gggaatgaat
     1501 gatgtttgag ttttatatgg cagaacttct tcgccaccgc tgggggcatc tgcgcttata
     1561 tcgtttcccc ggttctgttt tgaccgatta ccgaatactg aagaattacg ccaaaaccct
     1621 gacaggagca ggagtatgaa gtcagagata acaatcaact aatactgttt tgttgatttt
     1681 tgcttgtaat tggcgttctg gtctgatttt tgtggagtaa gttgatgcgt gatattcaga
     1741 tggttcttga gcgttgggga gcgtgggcgg ctaataatca tgaagatgtg acctggtcgt
     1801 ccattgccgc cggttttaag ggattaatta cttcaaaagt aaaatctcgc ccgcaatgtt
     1861 gtgacgatga cgcgatgatt atttgcgggt gcatggcccg tctgaaaaag aacaacagcg
     1921 atttgcacga tttattagta gattattatg tagtcggtat gacattcatg tcactggcag
     1981 gtaagcattg ctgctctgat ggttatatcg ggaaaaggtt acagaaggct gagggcataa
     2041 ttgaagggat gttaatggca ttagatatcc gg
//
LOCUS       DAZUCC010000401         2065 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZUCC010000401 DAZUCC010000000
VERSION     DAZUCC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.403"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..447
                     /locus_tag="ACHY09_005132"
     CDS             22..447
                     /locus_tag="ACHY09_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3887947.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSGAGVELLPVEITPDEQKEPVAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            444..794
                     /gene="tnpB"
                     /locus_tag="ACHY09_005133"
     CDS             444..794
                     /gene="tnpB"
                     /locus_tag="ACHY09_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS3887948.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            825..>2065
                     /locus_tag="ACHY09_005134"
     CDS             825..>2065
                     /locus_tag="ACHY09_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFS3887949.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     AGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTS"
BASE COUNT          522 a          526 c          573 g          444 t
ORIGIN      
        1 ttcaactagt ggacacctgt tatggaacag aaaatattat cctcagaacc ccgcagatca
       61 ttttcaaatg agtttaaact tcaaatggtt aaactggctt cacaaccagg agcctctgtt
      121 gcccgtattg cccgggaaca cgatatcaat gataacctcc tgttcaaatg gctcaggctc
      181 tggcagaacg aagggcgtat atcgcggcgt cttccggtaa caacctcttc tggcgctggc
      241 gttgaattat tacctgttga gataacgcct gatgagcaga aagagcctgt ggcggctctt
      301 actccgcttt tatccactcc ttcacaaagc acagtcagtg ccagttcctg taaagttgag
      361 ttccgccacg gtaacatgac gctggaaaat ccttcaccag agctgctcac ggtgttgatc
      421 cgtgaactga ccgggagggg acgatgatct cgctcccatc aggcactcga atctggctgg
      481 ttgccggcgt tactgatatg cgtaaatcct tcaacggtct gggagaacag gtacaacatg
      541 tgctggatga gaaccccttc tccggtcacc tgtttatctt tcgcggccga cggggtgaca
      601 cgattaaaat tctgtgggct gatgctgatg gtctgtgcct gttcaccaaa cgtctggagg
      661 aaggccagtt tatctggcct gcggtgcgtg acggtaaggt atccattacc cgctcgcagc
      721 tggcaatgct cctcgataaa ctggactggc gtcagcccaa aacatcccgc cttaatgcac
      781 tgacaatgtt gtaaaaatgt catggccgga ttataaaaac ggtcatgagt cggaaatacc
      841 tcattcgtat cactgagctg gaaaggctgc tctctgagca ggctgaagcc ctccgtcaga
      901 aagatcagca actgagtctg gttgaagaga cggaggcctt cctgcgctct gcactggcac
      961 gtgccgagga aaagatcgaa gaggaggagc gggaaataga acatctgagg gcccagatag
     1021 aaaaactgcg ccggatgttg ttcggtaccc gttctgaaaa actgcagcgt gaggttgaac
     1081 aggctgaggc ccaactgaaa caacgcgaac aggaaagcga tcgttacagt gggcgtgagg
     1141 atgacccgca ggttccccgc cagttgcgac agtcacgtca tcgtcgtccg ttaccggcac
     1201 atcttccccg tgaaatacac cgtctggagc ctgaagaaag ttgttgcccg gagtgtggca
     1261 gtgagctgga ttatctgggg gaagtcagtg ctgagcagct ggaactggtg agtagcgccc
     1321 tgaaagtgat ccgcactgtt cgggtaaaaa aagcctgtac aaaatgtgac tgtatagttg
     1381 aagcgccagc gccgtcccgc ccgatagagc gcggcatcgc gggctccgga ttacttgccc
     1441 gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt gaaatctttg
     1501 cccgccaggg catcgaactg agccgggcat tactctccaa ctgggttgac gcatgttgtc
     1561 agttaatgac tctgctgaat gataccctgt accgttacgt gatgaacacc cgcaaggttc
     1621 acactgacga cacaccagta aaagtgctgg cagcgggcag aaaaaaggca aaaacaggac
     1681 gcatctggac gtatgtccgg gatgaccgga atgcgggctc atcagagcca ccggcggtct
     1741 ggttcgccta ctcaccagac aggcagggaa aacatccggt acaacacctt cgtcccttcc
     1801 ggggtatcct gcaggcggat gcgttcagcg gttacgatcg gctgttcagt gcagaacgtg
     1861 aaggtggtgc actgacagaa gttgcgtgct gggcccatgc ccggcgaaaa atccacgatg
     1921 tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc agtgaactgt
     1981 acgccatcga ggatgaaata cggggattac cggagtcaga gcgtcttgcc gtcaggcagc
     2041 agcgaagcaa agtgttactg acgtc
//
LOCUS       DAZUCC010000402         2055 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZUCC010000402 DAZUCC010000000
VERSION     DAZUCC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.404"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..275)
                     /locus_tag="ACHY09_005135"
     CDS             complement(<1..275)
                     /locus_tag="ACHY09_005135"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS3887950.1"
                     /translation="MSELSYLEKLLDGVEVEWLPLGEVSALRRGRVMSKGYLTENFGP
                     YPVYSSQTANNGKIGSINTFDFDGEYISWTTDGANAGTVFYRTGKFS"
     gene            complement(272..1828)
                     /locus_tag="ACHY09_005136"
     CDS             complement(272..1828)
                     /locus_tag="ACHY09_005136"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS3887951.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSHMENGDDSICYAKLDDNVITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLNLGDFNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSARGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTRVQFIDASELFKKEANNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCGVQK"
BASE COUNT          532 a          488 c          450 g          585 t
ORIGIN      
        1 attgaaaatt tacctgttcg atagaacaca gtgcctgcat ttgcgccatc tgttgtccag
       61 cttatatatt cgccatcaaa atcgaaggta ttgatagaac ctattttccc attatttgca
      121 gtttgagagc tatagacagg atatgggcca aaattttcag ttaaatatcc ctttgacata
      181 actctgcctc ttcttaaggc tgagacttcc cccaatggca accactcaac ctcaacccca
      241 tccagcaatt tttccagata actcaactcg ctcatttctg caccccgcag ccttcaattt
      301 cagccacaat cgcatcaata tctttacgca actggtcgat tttgctgacc gtggttttca
      361 gctcagcatt cagctcagca atattgataa tttcacgagt atctttcgct tccacatagc
      421 tgctcaccga caggttatag tcattagcga caacggtctc aaacgcgact gatttcgcca
      481 gatgggcaac atcttccttg ctggcaaata cctgcataat ctgttcgata tgagcatcgg
      541 tcagaatgtt gttgttagcc tcttttttga acagttcgct ggcatcaata aactgaactc
      601 tggtatccgt tttatgttta gacagcacca gtatgtttac ggcaatggtg gtgccaaaga
      661 acaggttcgg tgccagtgaa atcacggttt cgacatagtt attgtcgacc agatactgac
      721 ggattttctg ctctgcgccg ccacggtaaa aaatacccgg gaagcagaca atcgcagcgc
      781 gacctctggc cgaaagatag ttcagcgcat gtaatacaaa cgcaaagtca gctttggatt
      841 tgggggccag aacgccagcc ggggcaaaac gctcatcgtt aatcagtgtc gggtcatcgc
      901 tgccagtcca tttcaccgaa tacggcgggt tagaaacgat ggcatcaaac ggtttttcat
      961 ctctgaagtg cggctcagtc agcgtattgc ccagcttgat atcaaacttg tcgtagttga
     1021 tgttgtgcaa aaacatgttc atacgcgcca ggttataggt cgtatggttg atttcctgac
     1081 caaaaaagcc ttcttcgatg atatggtcat caaactgctt tttcgcctgc aacaacagcg
     1141 aaccggaacc cgctgccggg tcgtagattt tgttaacgtg ggtctgcccg tgcatagcca
     1201 gttgtgcaat cagcttagag acgtgctgtg gtgtaaagaa ctcgccgcct gacttaccgg
     1261 catttgcggc atagttagaa ataaggaatt cataggcatc gccaaacagg tcaatctgat
     1321 gtttgttgaa gtcaccaagg tttaaccctt caaccccttt cagaacagca gccaggcggg
     1381 catttttatc cttaacggtg ttacccaggc ggttactggt ggtatcgaaa tcagcaaaca
     1441 aacccttgat gtcagcttct gaaggataac cgtaagcaga actttcgata gcaacgaaga
     1501 tgctgtttaa atctgcattc agtctgtcat tggtatttgc tttcgcagct acgttgcaga
     1561 aaagctgact ggggtagatg aagtagcctt tagtcctgat ggcatcgtct ttaatgtcat
     1621 cagtaattac gttgtcatcc agttttgcat aacagatgct gtcatcacca ttttccatat
     1681 ggctggaaaa attttcgctg ataaaacggt agaaaagtgc gcccagaacg tattgcttaa
     1741 aatcccatcc atcaacagaa cctctgacat cattggcaat ttgccatatc tggcggtgca
     1801 gttctgctcg ttgttgaata cttgtcattt ttaatccatt ttcctggtct ggacctgaat
     1861 cattgggggc aattctacag caatcttgtg gttcaggcag ccccggagta agtcggtgta
     1921 cattgtgaga tacctggggt attcacgaca aggaggagga acggaatact gcctttcctt
     1981 tgcagtgcac caggtacatt ctaaataatt tcagacagat attatcgttg tgagtccccc
     2041 gcctcctttc cggtg
//
LOCUS       DAZUCC010000403         2043 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZUCC010000403 DAZUCC010000000
VERSION     DAZUCC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.405"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..341)
                     /locus_tag="ACHY09_005137"
     CDS             complement(<1..341)
                     /locus_tag="ACHY09_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS3887952.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLAR"
     gene            complement(1123..1812)
                     /locus_tag="ACHY09_005138"
     CDS             complement(1123..1812)
                     /locus_tag="ACHY09_005138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887953.1"
                     /translation="MVFQESNNRDVARKYAEYITSVPLRRFVAEKVREYCGDLPLSIF
                     DGACGSGQLEQFLTVGSLYAVDIQASPLLHIPDNFPDATVKTVNENFFNAIDDVSDTD
                     TFDCVIMNPPFSLQYKAQSADIREKISAMMPYKKTSGVLDDAFYVLASMKARFSFFCA
                     FPGLVTGVVSWQCVSILATALLSWFRFQAGSLIHRFLYYSSLLITKSKAMMLLFMASG
                     LMGKLAKSQRR"
     gene            complement(1898..1974)
                     /locus_tag="ACHY09_005139"
     tRNA            complement(1898..1974)
                     /locus_tag="ACHY09_005139"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:tct)
BASE COUNT          542 a          567 c          472 g          462 t
ORIGIN      
        1 cgcgccagct gcttaatacg cgggtccgga cggtcatatg tctccggcag cggaaggcct
       61 tcaccatacg acatgccgtt tgactgccct gccagaacca caacaaagta atactccggg
      121 tcgctggtgg cgctgattac tgtgccttct ccatccgacg gcttcaccac cacaggtgtg
      181 gtgacatcac cttccgccgc aatggcctgc atcagggtat aaggcgtgat ggccaccgga
      241 ctgccaaatg gctgccaccc ctccttcagt ttttgagtca gtcgctccgc aaggtctgac
      301 ggcgacgccg ccctgaccac gtcatagtgt ttaaatgcca tgaatcctcc cggtcgggat
      361 aatgttgtga gtcagatgag gggcgggctg aagtccggaa gttacaggac aatggcagga
      421 ggaagactac agcccgcaat acgaaaaagg ccgcgcagtt gcgcagagtg attactgtcg
      481 ggtattattc gccagctgaa aaattgatta cttcacgttt tgttgtttat tccttgctgc
      541 ccgcatctca ccatgcgggc tttttttatc cataagaaag cccctccgga gaggggctga
      601 agagtagcgc tatgtaccgt tcattacatg atgccgggtg cctcccggtg agttcagtat
      661 cagcacctga acccgcacag aaaggataag ggatgtaacc aaacacctgt cgctgatatg
      721 cccctccgct cagggggatt caccatgcca gtttctttta acaaactccc cgcaaatcag
      781 acaactgtca accgtctgaa ttgtgagaca tttaaaattt tcggggcaac aggaagacaa
      841 tcatcacgac accggcaacc agcgaccggc accagattta acgtatgtgc acacaacctg
      901 aaccggcatc aaaaaacaaa ccgtgcactg acagaaaacg ggaaacctcc gtccgctgat
      961 catcactcat taaggaccac acaaaggcgt tatgctcatc ttcaagtttc cgtcttcttg
     1021 tttgccttac aagcaactca gccgtcacct gtacaatgtc aatgacctcc ttctcttcag
     1081 caatgtacgg cctgttccag ttgtctgtgt taatcttctc cgtcaccggc gctgactttt
     1141 tgcaagtttc cccattaaac ccgacgccat aaacaacaac atcatcgctt tgcttttcgt
     1201 tatcaataat gacgaataat acagaaaccg atgtatcagc gaacccgcct gaaatctgaa
     1261 ccaactcaac aacgcggttg ccaaaatact gacgcattgc cagctcacca cgcctgtaac
     1321 caaccccggg aaagcacaaa aaaatgaaaa tcttgccttc atgctcgcca gaacataaaa
     1381 cgcgtcatcc agaacaccgg aggtcttttt ataaggcatc atcgctgaaa tcttctccct
     1441 gatatccgcg ctctgtgctt tgtactgtag cgaaaaaggc ggattcatga tgacacaatc
     1501 gaacgtgtcc gtgtcactga catcatcgat ggcgttgaag aaattttcat tgaccgtctt
     1561 cactgtggca tccgggaaat tatccgggat atgcaacaac ggagaggcct gtatatccac
     1621 agcatacaga cttcccaccg tgagaaactg ctccagttgc ccggagccac acgcaccatc
     1681 aaaaatactc aggggcagat caccacaata ttcccgcact ttttcagcga caaaccgacg
     1741 caaaggaacg gacgtgatgt actctgcgta ttttctggcg acatcgcgat tattggactc
     1801 ctgaaaaacc atttgtgaaa ggctctgtgg ttgatatgcg ccgggcgtgg cgcggaggca
     1861 aaaaaggccc acaaaagcga gccaggaaaa ataagtgtgg tgcgttgtac tggattcgaa
     1921 ccagtgaccg attgcttaga aggcaattgc tctgtccggc tgagctaaca acgcagtgta
     1981 cagataatgg accgccatcg aggactcgaa ccccgcgcaa ccagcttcga aggctggcgc
     2041 tct
//
LOCUS       DAZUCC010000404         2039 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZUCC010000404 DAZUCC010000000
VERSION     DAZUCC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.406"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            15..2000
                     /gene="yjcS"
                     /locus_tag="ACHY09_005140"
     CDS             15..2000
                     /gene="yjcS"
                     /locus_tag="ACHY09_005140"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFS3887954.1"
                     /translation="MNNSRLFRLSRIVIALTAASSMMVNTANATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETI
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAKEANKQGDYRWSAELLKQVIAANPGDQVAKNLQADNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMV
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          537 a          535 c          522 g          445 t
ORIGIN      
        1 aaatggagat ttatatgaat aactctcggt tattccgttt gagcaggatt gttattgcgt
       61 taactgccgc cagcagcatg atggtaaata ccgctaatgc aacagatgaa gcgaaagccg
      121 ccactcaata tacccaacag gttaatcaga attacgccaa atcattaccg tttagcgatc
      181 gtcaggattt tgacgatgct cagcgtggat ttatcgctcc gctgctggat gaaggtattc
      241 tgcgtgatgc gaacggtaaa atttactacc gcgcgaatga ttacaaattt gatattaatg
      301 ccgctgcgcc ggaaaccata aaccccagcc tgtggcgtca gtcgcagatc aacggcattt
      361 caggcctgtt caaagtcacc gataaaatgt atcaggtgcg cggccaggat atctctaaca
      421 ttacgttcgt tgagggcgag aaaggcatta ttgttatcga cccgctggtg acgccgcctg
      481 cagcaaaagc cgcacttgat ctttacttcc agcatcgtcc gcaaaaaccg attgttgccg
      541 ttatctacac tcacagccac gccgaccact atggcggcgt gaaaggtatt atctctgaag
      601 ccgatgttaa atccggcaaa gtgcaggtga ttgctccggc aggctttatg gacgaagcca
      661 tcagcgaaaa cgtgctggcg ggtaacatca tgagccgccg tgcgctctac tcttacggtc
      721 tgttactgcc gcacaacgcg caaggcaacg tgggcaacgg cctgggcgtg acgctggcaa
      781 cgggcgaccc gagcattatt gcaccgacta aaactatcgt cagaactggc gagaagatga
      841 ttatcgacgg cctggaattt gacttcctga tgactccggg tagcgaagcg ccagccgaaa
      901 tgcacttcta tattccggcc ctgaaagccc tgtgtaccgc cgagaacgcc acgcataccc
      961 tgcacaactt ctacactctg cgcggcgcga aaacccgcga taccagcaag tggaccgagt
     1021 acctgaacga aacgctggat atgtggggta acgacgcgga agtactgttt atgccgcaca
     1081 cctggccggt ctggggcaat aagcatatca atgattatat tggtaaatat cgcgatacca
     1141 tcaagtacat tcacgaccag accctgcacc tggcgaacca gggctacacc atgaatgaaa
     1201 tcggcgacat gattaagctg ccgcctgcgc ttgccaataa ctgggccagc cgcggctatt
     1261 acggttctgt cagccacaac gcccgcgcgg tgtataactt ctatcttggc tattacgacg
     1321 gtaacccggc taacctgcat ccgtatggtc aggtggagat gggtaaacgt tacgtgcaag
     1381 ctctgggcgg ttctgcccgc gtcatcaacc tggcgaaaga agcgaacaag caaggtgatt
     1441 accgctggtc ggcagaactg ctgaaacagg tgattgccgc caaccctggt gaccaggtcg
     1501 cgaagaatct gcaagcggat aactttgaac agctgggcta tcaggccgag tccgccacat
     1561 ggcgcggttt ctacctgacc ggcgcgaaag agctgcgcga aggggtacat aagttcagcc
     1621 acggcaccac cggttccccg gacaccattc gcggaatgtc ggtcgaaatg ctgttcgact
     1681 ttatgtccgt tcgcctcgat agtgcgaaag ccgcgggtaa aaatatcagc ctgaacttca
     1741 atatgagcaa cggcgataac ctcaacctga cgctgaacga tagcgtgctt aactaccgta
     1801 aaacgctgca acctgaagcc aacgcctctt tctacatcag ccgtgaagat ctgcatgccg
     1861 tgctgaccgg acaagtcaaa atggtggatc tggtaaaagc gaagaaagcc aaaattattg
     1921 gcaatggcgc gaaactggaa gaaattattg cctgtctgga taatttcgat ttgtgggtga
     1981 atatcgtaac cccaaattaa tgctactcac ttttgggggc gcttaagccc ccaaaagca
//
LOCUS       DAZUCC010000405         2027 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZUCC010000405 DAZUCC010000000
VERSION     DAZUCC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.407"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(38..1510)
                     /locus_tag="ACHY09_005141"
     CDS             complement(38..1510)
                     /locus_tag="ACHY09_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3887955.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            complement(1559..>2027)
                     /locus_tag="ACHY09_005142"
     CDS             complement(1559..>2027)
                     /locus_tag="ACHY09_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS3887956.1"
                     /translation="IVVNRKLLTGVSGLAGHVGHILSGVTDTECGCGRRGCVEAVSSG
                     RAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSASTIAELIVSLKLLLDCQVVV
                     VGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSDSGLIGATLWDRDCIT"
BASE COUNT          691 a          509 c          332 g          495 t
ORIGIN      
        1 tgtaatgaat cattttttta ttccctgata gcatccttta ttctcctgca acattctttt
       61 ttcttgctcc ataaaaaggt tttccatcga cagtatcata ttcaagcgca gcttctggat
      121 ggatccatct ctgtacacgt gatggcatat caaaaccaat aaggacaaga acaacaaatg
      181 ttaagataaa agcaagagaa cacattgctg taccaagact tgtatgagac gcaacaactg
      241 caccaagaat aggcgcacat gctccaccaa gtgaacctac attataaata aaccccagtc
      301 ctgccgcacg ctgctccaca ttaaaataac cactgattat tttcggcacc agagcaccaa
      361 tcccctgacc aaaaacctgc tgagtaaaga tcagaaggcc aagtaaccat acatagtccc
      421 ggtccacaag aaaaacgggg ataataacaa gctgaccgat aagaagtgat ataacatatg
      481 cttttcgggt tccaagccag tcccccataa aaccacctat acaacttcca gctgcagagc
      541 caaaacctgc cagcataaat atgagagcaa cagtttctgg tgaatactgc atatcaacct
      601 taagccatgt aggaagaaaa gcctgcagtg gccatccgta gaggaaacag aacataacca
      661 caagcatcaa cataatgcct gttggccatc ttttccccat accctgatat ataaaactga
      721 taaaaacagc cgcacagcac aatgccataa ctgtgattaa agcagcgttt gcagtgacgc
      781 ctgaaaaaca aacatataga catgcaaatg caatacaaga cagaagaata tttatgtatt
      841 tattcttttc tctgtacaga atatccacca ttgttctgac gggtttattc tcttgttgcc
      901 ttttttgcca gtcatcagac tccggaagat tcttgcgaag ataaaaagca aataatattg
      961 gaagcatgcc gacaaagaaa agagaacgcc agccccatag tggtactaca atgctataaa
     1021 cctgtgcagc taaacctccc ccaatagaaa atccagaaat taaaaaacca cttgccttgt
     1081 ttctgagatg aacaggccag ctttcgataa catatgttac acttgagcca tattcacctg
     1141 ccataccaag cccaataaca atgcgagcaa taaacatcac tgcatatcca ggagccagac
     1201 cacaggcaag ggttccgaga gagaatagca ctatacttaa aaccattgcc atcctgcgcc
     1261 ccatcttgtc cgataaagcg ccaatagcaa gccccccaaa ccatcgggaa ataaaagcag
     1321 cggaaataag gcttgccgct tctattgtcg tcaagccgaa ttcatgctga acctctgtaa
     1381 gaaccagaga tatcagaacg aaatcaaaac catcaagtaa ataacctgtc catgccgcac
     1441 cgaatatctt ccactgtctg ggtgaaagat gcttatacca aacctgcttt ttctgaatac
     1501 aggagtccat attaaacctc acaataatag cggatttata atacactgga gtattacttt
     1561 aagtaataca atcccggtcc caaagagttg caccaataag accagagtca cttctgaaat
     1621 atgccggaaa cagcattaca ttacaaattt cagaatatat ggaaagatgt ttcgacactt
     1681 tctgaatata cccatcagca agtccaacac ttcctccgac aacaacaacc tgacaatcaa
     1741 gcagtaattt taaacttact attaattcag caatagtact ggcagaacgc tctgtcaaaa
     1801 attcggcttc cttatatccc tgtctcgcta attcaaaaat atatttggtg ctatatcctg
     1861 caagttcatt ttttgctgcc cccatgatgg cccgaccaga tgaaaccgct tcaacgcatc
     1921 cccgacgacc acatccacat tctgtatccg ttactccgga caatatatgc ccaacatgtc
     1981 cggctaatcc agaaacacct gttaataatt ttctgttaac tacaatt
//
LOCUS       DAZUCC010000406         2009 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZUCC010000406 DAZUCC010000000
VERSION     DAZUCC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.408"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(188..1600)
                     /gene="pykF"
                     /locus_tag="ACHY09_005143"
     CDS             complement(188..1600)
                     /gene="pykF"
                     /locus_tag="ACHY09_005143"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFS3887957.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1608..1802)
                     /locus_tag="ACHY09_005144"
     CDS             complement(1608..1802)
                     /locus_tag="ACHY09_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887958.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          511 a          457 c          494 g          547 t
ORIGIN      
        1 catcgaacgc tgggttttag ataaaaaaat tgagagaaaa caggatgctt ccatcggatt
       61 catcttagat aaagtgagat tatatagtga aatccgattt gattttttat tgcttctggt
      121 tatcgattaa ataaaaaaag cgcccatcag ggcgcttcga tatacaaatt aattcacaaa
      181 agcaatatta caggacgtga acagatgcgg tgttagtagt gccgctcggt accagtgcac
      241 cagaaaccat aactacgacg tcacctttgt gtgccagacc gctctgcaga gccagttctt
      301 tacccagacg gtagaaatca tcagtagaag tgatctcttt aaccagctgc ggcacaacgc
      361 ctttgctcag taccaactga tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg
      421 catccgggaa gtatttacgt actgcgcgag cagatttacc gccctgggta gcaaccacga
      481 tcagcggggc atccagtttt tcagcagttt caacggcacc acggcatacc gcttcggtaa
      541 tgcgcagttt acggttgtca ttgttgaact cgagacggct gttcatcacg cggtcggtac
      601 gttcgcagat ggtcgccatg atagaaaccg cttccagcgg gtatttacct tttgcggatt
      661 caccagacag catcactgcg tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt
      721 ctgcgcgagt cgggcgtggg tttttaatca tggaatccag catctgggtc gcagtgataa
      781 cgactttacg tgcacggata catttttcga tcatcatctt ctgggcgaag ataacttctt
      841 ctaccgggat ttctacaccc aggtcgccac gcgcaaccat gatgccgtca gaggcttcga
      901 ggatttcgtc gaagttgttg aggccttcct ggttttcgat tttggagatg atgtggatgt
      961 tttcgccgcc gtgcgctttc aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa
     1021 taaaggaagc agcaacaaag tctacgcctt gttcgcaacc aaagatcagg tcctgtttgt
     1081 ctttttcagc cagtgctggc agggcaatgg aaacgccagg caggttcaca cctttgtttt
     1141 cacccaggtc gccgttgttc agcactttac agataacttt gttaccttca atggcggtaa
     1201 cttccatacc gatgagacca tcgtcaacca gtacggtgtt gccaacagac aggtcagtag
     1261 tgaaaccttc atacgttacc gcaaccattt cgctgttgcc gataacagat ttatcagtgg
     1321 tgaaagtaaa ggtctgaccc gctttcagag aaacgtcgtt accgccttcc agtttcatgg
     1381 tgcggatttc cggacctttg gtatcaagca ggatagcggc ggttttacca gttttgctca
     1441 tcacgttgcg cagattctga atgcgctgac cgtgttctgc atagtcacca tgagagaagt
     1501 tcagacgcat aacgttcatg ccagcgtcca gcattttagc taacatctct tcagattcgg
     1561 ttttcggtcc gatggtgcaa acaattttgg tctttttcat gacagtctta gtctttaagt
     1621 tgagaaggat gggagaaact tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta
     1681 cgaaagtggt ggtgcctcgc tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg
     1741 tgtgcttgtg tctcagccca acgtgagtgc ctgtttttga gtcgttgagt tctacagtcc
     1801 aaagtgctga aaccattcaa gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa
     1861 aggaaaacaa aaacagcgtt tcaaaaaata tacaacttgt tagacaataa attgttatca
     1921 aagatttaac gttccaggga aaaggttacg caaaaacttc gctgtttgcg caacgtctgg
     1981 tgccggggtt gtcactgtga agataagtg
//
LOCUS       DAZUCC010000407         1995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZUCC010000407 DAZUCC010000000
VERSION     DAZUCC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.409"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..654
                     /locus_tag="ACHY09_005145"
     CDS             <1..654
                     /locus_tag="ACHY09_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS3887959.1"
                     /translation="IYLDFFAGSGTTAHGILELNIEDGRKRNFICVQLDEETDKKKQA
                     YKEGYKSIFNITKDRIIKAGKKLKKDNPNYNGDLGFKIFETVHDFRAKDESELTLSNL
                     SFFDDAVLTPEQYDTLLTTWCVYDGSLLTTPIEDIDLNGYKAHLCDGRLYLIAPNFTS
                     EALKALLQKLDADKDFAPNKVVFYGSNFESAKQMELNEALKSYANKKSIDLDLVVRN"
     gene            657..>1995
                     /locus_tag="ACHY09_005146"
     CDS             657..>1995
                     /locus_tag="ACHY09_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS3887960.1"
                     /translation="MSKGFTFEKNLPHQKAGVDSVMNVFVSAIPHQTDHVAIRLLANP
                     ELNLSEQQYYNNIKNVQEFNGIEHSKDNYDAKSNVIDVSMETGTGKTYTYTKTIFDLN
                     KSFGINKFIIIVPTLSIKAGTVNFLKSDAIKEHFRDDYERELKTYVVESQKSSGKNTK
                     SYMPQAIHDFVEASNFNKKYIHILVINSGMINSKSLTDTYDVGLLDNQFDTPFSALSA
                     IKPFIIIDEPHKFPTGKKTWEHIQKFNAQYIIRYGATFSEGYKNLVYRLTAVDAFNED
                     LVKGIDAYIEDIVGDGNANLKLVKSDGKEATFELNENNNKKIFKLAKGESLSKTHSAI
                     HDLTLDALNKNTAVLSNGIELKIGSSINPYSYDQTLADNMMRKAVKEHFKLEKEYLTQ
                     RPRIKPLTLFFIDDIEGYRDGNDIAGSLKTKFEEYVLAEANELLKTEKDEFYRN"
BASE COUNT          711 a          335 c          369 g          580 t
ORIGIN      
        1 atttatcttg acttttttgc tggctctgga actacagctc atggtattct ggagttaaat
       61 attgaagacg gcaggaaaag aaacttcatc tgtgttcaat tagatgaaga aacagataaa
      121 aagaaacaag cctataaaga agggtataaa tccatcttca atataacaaa agacagaatt
      181 attaaggcag gaaaaaagct gaaaaaagat aatcctaatt ataatggaga tttgggattt
      241 aaaatatttg aaacagttca tgattttcga gcgaaagatg agtctgaact tacgctttct
      301 aatcttagtt tctttgacga tgctgtattg acaccagagc agtatgacac tctattgacg
      361 acatggtgcg tgtatgatgg cagcttgcta acaacaccaa ttgaagatat tgaccttaat
      421 ggctacaaag cacacttgtg cgatggtcgg ctatatctaa ttgcgcctaa tttcacaagt
      481 gaggctttga aagctttact tcaaaagctg gatgcagaca aagactttgc ccctaacaaa
      541 gttgtatttt atggcagcaa cttcgaaagt gcgaaacaga tggaactcaa tgaagcacta
      601 aaaagttatg ctaataaaaa atctattgat ttagatttgg tggtaaggaa ctaatcatgt
      661 ctaaagggtt cacattcgaa aaaaatttac ctcatcaaaa ggctggcgtt gattcggtaa
      721 tgaatgtctt tgtatccgcc atacctcatc agacggatca tgttgcaatt cgcctgttag
      781 ctaatccaga actgaatcta tctgaacaac aatactacaa caacataaaa aatgttcagg
      841 agttcaatgg tattgagcac tcaaaagata attacgatgc taaaagcaac gtaattgatg
      901 tttctatgga gacaggtaca ggtaaaactt atacttacac caaaacaatt tttgacctga
      961 acaaatcatt tggtattaac aaatttatca tcattgttcc taccctatcg atcaaagccg
     1021 gaaccgttaa ctttttaaaa agcgatgcta taaaagaaca ctttcgagat gattacgagc
     1081 gtgaactaaa gacttacgtt gttgaaagtc agaaaagttc gggtaaaaat acaaaatcgt
     1141 atatgcctca agctatccat gattttgttg aagcaagtaa tttcaataag aaatatatac
     1201 acattcttgt gatcaactca gggatgatta actctaagtc tttaactgac acttatgatg
     1261 ttggcttgct tgataatcag tttgataccc cattttctgc acttagtgcg ataaaaccat
     1321 tcattatcat tgatgagcct cataaatttc ctactggtaa aaaaacatgg gaacatattc
     1381 aaaaatttaa tgcccaatat atcatccgct atggcgcaac ctttagtgaa ggctataaga
     1441 acttagttta tcgtcttaca gccgtagatg ccttcaatga agatcttgtt aaaggtattg
     1501 atgcatacat tgaggatatt gttggtgatg gtaatgccaa cctcaagttg gtcaaatctg
     1561 acggaaaaga agctacattt gaactgaacg aaaataataa taaaaaaatt tttaagttag
     1621 ccaaaggtga atcactatct aagacgcata gtgcgatcca tgatttaact cttgatgctt
     1681 taaataagaa tacagcagtg ttgagtaatg gtattgagct aaaaattggt agctcgataa
     1741 acccctattc ttacgatcag acacttgctg ataacatgat gcgtaaagct gtcaaagaac
     1801 actttaagtt agaaaaagag tatctgacac aaaggccacg cataaaacct cttactcttt
     1861 tcttcattga tgacattgaa ggctatcgtg atggtaatga tattgcagga agcctgaaaa
     1921 ctaagttcga agaatacgtt ttggcagaag caaatgaact gttaaaaaca gagaaagatg
     1981 agttttacag aaact
//
LOCUS       DAZUCC010000408         1993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZUCC010000408 DAZUCC010000000
VERSION     DAZUCC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.410"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..585
                     /locus_tag="ACHY09_005147"
     CDS             <1..585
                     /locus_tag="ACHY09_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFS3887961.1"
                     /translation="KNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATESVSSGISSP
                     AVETHIPEAPARKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRAEGGGFLPLKT
                     GITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRYNLDGQPEGEV
                     SECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
     gene            complement(806..>1993)
                     /locus_tag="ACHY09_005148"
     CDS             complement(806..>1993)
                     /locus_tag="ACHY09_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3887962.1"
                     /translation="LPGINPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSD
                     TGIQENITEKQRVLNKINSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAY
                     SYGTQIAALYAERFPSSIRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCA
                     RTKSCPLSSVRDKAITQFHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWP
                     TLATAIRQFSQGIVSNEIETALSTPIASEESSDALGVILCVDQGGEQLTPEERKSRKD
                     ALANAFPAINFDNGRSDSPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPW
                     INARKMAEKFSSPLLTINGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEI
                     DIK"
BASE COUNT          549 a          445 c          452 g          547 t
ORIGIN      
        1 aaaaatcccg tcattgtggt gaaaaaacgt cgctcttttt caccgccttc cttagccgaa
       61 aaaaaagatg ttatcgcgcc tgcactcact gaacaggcga cagaatcagt ctcctccgga
      121 atcagttcac ctgccgtcga aactcatatc cccgaagcgc cagccaggaa gaagaaaaag
      181 aaaagacaac gctttccaag accatcccac tggaccagtg agttcactca cgattgtctg
      241 gagaaggtga aagcgctgtt tcctcaactt cgtgccgaag ggggcggttt tctgcctctg
      301 aaaacaggca taacccgtga cattactaca tatctggcag cacatccgga aacagcgctg
      361 accctggacg aatggaattg tgcagtatcc tgcattacca gtcgccggat ctacctgcag
      421 agaacctctg ttgcaggaac tccccgttat aacctggatg gtcaaccaga gggggaagta
      481 tcggaatgtg atgctcaaaa ttccaggcgc tggctggcgg cgaaagagca gaggtggctg
      541 agtatgaagg cccggcagga aaatatttct gagaaatcaa aataaccgca ggatattaca
      601 ggaaacagac tgtagagaag agagcgttgt atattgtgcg tattatttaa gcactcccga
      661 gagtaatggc ttgattttgc gcgatcgcga cattttttac gtgtatccag tttgacgttt
      721 ctggtgccgt gatcgcactg aacgcatttt gctcccaatg caaaatatgt ccggtatcag
      781 gagcaaatgg cgggcaattt ttaaactatt ttatatctat ttcagcattt cctggacagg
      841 atatatttcc cattttcata ggggtgataa gatgacgaac aacggcctta tctacacata
      901 aattgactcc ggtaagagct aatgtatgcc catcaccatt tattgtcagc aacgggctgg
      961 aaaatttctc tgccatctta cgagcattaa tccatggtgt tgttggatcg tatttgtgtg
     1021 ctacaaacag taaaccagag ggcagaactg tatttttcag ccgggtttta ttcaggttgc
     1081 tatgtattgg ccataattca caaaaatcag gtgaatcgga acgtccattg tcaaagttaa
     1141 tagccgggaa ggcattcgca agagcatctt ttcgggattt tcgctcttct ggggttaatt
     1201 gctccccccc ctgatcaaca caaaggatta cccccagagc atcacttgac tcttctgagg
     1261 ctataggagt actgagcgca gtttcaattt cattactgac aatcccctga gagaactgac
     1321 gtatggcagt tgcaagggtt ggccatgatg aacgccataa caaaaggtct gttgttaata
     1381 atatgagttc atctgaagat atattttctc ccttgctgtc taataaaggc ttgtgatgca
     1441 attttgataa cagctcatgg aactgagtta ttgccttatc tctgactgaa gaaagtgggc
     1501 aactttttgt acgtgcacac caggatgcaa agcgatcgaa cgtttcctga taactctgtg
     1561 cttgtttgag ttgccatgtg aagttgtccc ccaggtcatc aatatcgacg accccatcaa
     1621 gaacgataga tctgatgctg gatgggaaac gttctgcata taaggctgca atttgtgttc
     1681 catacgaata tgccacagct gtcagttgct cgtcacccag ggcttgcctg atacgatcaa
     1741 tatcatatac cgcctcgtta gaaccaatat ggcgaataac ttcagttccg gtattatgga
     1801 tacagctgtt aattttattt aatacccgtt gcttttcagt tatgttttcc tgaatccctg
     1861 tatctgattg ctggcagttt attgcaggaa tggactggcc gactccccgt ggatcaaagc
     1921 caataatatc ccatgattcg cgaagatttg ttaccggcca gtcaaagtta atataaggat
     1981 taatgccagg taa
//
LOCUS       DAZUCC010000409         1991 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZUCC010000409 DAZUCC010000000
VERSION     DAZUCC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.411"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..766
                     /locus_tag="ACHY09_005149"
     CDS             <1..766
                     /locus_tag="ACHY09_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HFS3887963.1"
                     /translation="LLWLAADEAGYVSNDWLTYRQAQQLGGHVRKGEVATLAVIYKDW
                     KKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHLMVKAEGSADALPA
                     DTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQVADYWTTLLHELV
                     HSTGHEKRLRRVGISTFTGRNSPEYAFEELIAEMGSAFLCAHLGIAGDVQHESYIDGW
                     LKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
     gene            876..1310
                     /locus_tag="ACHY09_005150"
     CDS             876..1310
                     /locus_tag="ACHY09_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFS3887964.1"
                     /translation="MNDNALVNVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKDGLLSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            1314..1628
                     /locus_tag="ACHY09_005151"
     CDS             1314..1628
                     /locus_tag="ACHY09_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887965.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKEELWYYNLFINPSADELMNVNRRYGPERTAWLQGFIRNN
                     GA"
     gene            1712..>1991
                     /locus_tag="ACHY09_005152"
     CDS             1712..>1991
                     /locus_tag="ACHY09_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFS3887966.1"
                     /translation="MSEWRKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAV"
BASE COUNT          479 a          446 c          599 g          467 t
ORIGIN      
        1 gttgctgtgg ctggccgcgg atgaagccgg gtatgtcagc aatgactggc tgacgtaccg
       61 tcaggcgcaa caactgggtg gccacgtccg taaaggggag gtggccacgc tggcggtgat
      121 ttacaaagac tggaaaaaac aggctgtgga tgaacacggt aaccggctga cggacagtga
      181 gggggagcct ctcatggaaa tcgtaccggt actgaaatcg aatcctctgt ttaatgtggg
      241 gcagtgtgac ggtttacctg aacatctgat ggtgaaggcg gaaggcagcg cggatgcact
      301 gccggcagat accttcagtg agcgggtgct gcaggtactg aatggcagcg gcgtcagcgt
      361 ctcgtcactt ccacaggaca gggcattcta ccggcctgat cttgaccgta tcgtgatgcc
      421 actgtcgtcg cagtttgacc aggtggctga ttactggacc accctgctgc atgagctggt
      481 acacagtacc ggtcatgaaa agcgcctgcg gcgggtgggg ataagtacct ttaccggtcg
      541 caacagcccg gaatatgcgt ttgaagagct gattgccgag atgggcagtg cttttctgtg
      601 tgcacatctc gggattgccg gtgacgtaca gcacgaaagt tatattgacg gatggcttaa
      661 ggttctgaag tctgacaaaa aagcgctgtt tcgcgcctgc cggcaggcac gtgaagcatc
      721 agagtattta ctgacgcggc gaacttcgca ggagtgcgca gcataatttt tactaccggt
      781 ggcggttata acctgaccgc catttatccc gtgagggcat tgtccctcac ggggcagtgc
      841 tctctgttat ttcaggatta cagaggaact gagtgatgaa cgataacgca ctggttaatg
      901 ttgacgatgc tgttgtggcc cgtatcaggg atgtgctggc gtatacattt ccgggacaga
      961 aattttctgt gcggaaatgc gatccatctt tcccgaaagt tttcaacaat gtgtgggtga
     1021 gctggctgga tggtccaacg gaggccgaag tacgctctgt cacagagggg tttgagaata
     1081 aagacgggct gctgtctgtg cttgaatccc gttatttcag cgatgaactg gtacaggagt
     1141 gtattgatcg ccttcgtaaa gagtacggac aggggaacgt cccgttgagt gtgaccgtgg
     1201 agcgttaccg gaaaaacgag ctggagaaga ttgaaacagc gcgttttccg ggcaacatat
     1261 ccgtagccat aaacgagatc gggataaata ccagcaaata caggaggtaa ggtatggctg
     1321 gtaaatgcct tgtctggttc tgtaacggga tctctgaacc gtgtctgata gcaggaaata
     1381 tcagtgaatt actggcagat gttacgatca cgtcaaatta ccgtggagat gtcacatctg
     1441 aaatcaggga tgttgtacag gagtggctca ggaccggcag ggggaacctg gcggatttaa
     1501 aggaagagct ctggtactac aaccttttca tcaatccgtc tgcagacgaa ctgatgaatg
     1561 tcaacaggcg gtatggccct gagcgaactg cctggctgca gggttttatc cgcaacaacg
     1621 gggcgtgata ccccgttttc ccgtgagggc attgtacctc acgggacaat gccctctttt
     1681 tacatctgta taaaaaagag gagcgtaaac aatgtctgaa tggcgcaaaa accgttttga
     1741 aattaccggt aaatcagtct gtcttgatgt acttacacag tggattgaag ggcgtgagac
     1801 cccgcgctac cgtcatgcca tacagcagag tatacagctg tttctggccg gttgtgcagg
     1861 gctggtaaaa cccacacgga cgacgcagta tccgccatat ccggcgctgg ttcgtcacgg
     1921 aacggggttg tcatctccgg ctaaccaggc gtttgagcag tggctggggt tgctggtaaa
     1981 aaatgccgtt c
//
LOCUS       DAZUCC010000410         1973 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZUCC010000410 DAZUCC010000000
VERSION     DAZUCC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.412"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(92..379)
                     /locus_tag="ACHY09_005153"
     CDS             complement(92..379)
                     /locus_tag="ACHY09_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFS3887967.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(454..597)
                     /gene="mgrB"
                     /locus_tag="ACHY09_005154"
     CDS             complement(454..597)
                     /gene="mgrB"
                     /locus_tag="ACHY09_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFS3887968.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            756..995
                     /locus_tag="ACHY09_005155"
     CDS             756..995
                     /locus_tag="ACHY09_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFS3887969.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1139..1930)
                     /gene="kdgR"
                     /locus_tag="ACHY09_005156"
     CDS             complement(1139..1930)
                     /gene="kdgR"
                     /locus_tag="ACHY09_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFS3887970.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
BASE COUNT          556 a          480 c          406 g          531 t
ORIGIN      
        1 taatttgacg cagttgcacc atgacagtgc gtataatcag gatgaattga gtaacccttc
       61 aacgacgggg ttactcaaga ttgaggaaag attatcgttc ggcgaccaga cgaataatat
      121 cttcgtcttc aaccgatttt tgactctcaa cggtcttttt atctgctttc tctggttcgt
      181 ttgcttcgca caaacgacgt aacagagcat tttgacgttt ttgctgatcc agcaacgcct
      241 caagcagttc aatctgttcg ttagtccgtg aactggcacg attgataaaa aaccacaaga
      301 taagcccgat aaaaagaacc accaccgata caaccaaaga cgcaatgttc atcacgcctg
      361 aatttacaac ttcgttcatt tcaccacctc tgagtagagg cgctattcta ccactgctgg
      421 agaggaagaa aatctagtgc tgaaaaaatg atatcaccac gggataaact ggttaatggc
      481 acaaattccg ctgaaaaatt gtacatcctg atcgcacatc atgttgaata cctgcgccca
      541 aagcagcaag caagccaaca ccacgacaac cagaacgacc catcgaaact ttttcactcc
      601 aatctccgtt tcacctacct tatgtcatta aactagcatg cgttagttaa acagcgccta
      661 actatgtcga tattcgtgct ttttcagaat gagtcgcttg cttcagcgca tattgccgct
      721 acgattaagc gaacataaaa agagaagagg ttgtaatgcg attcatcatt cgcacagtta
      781 tgctgatagc actggtatgg ataggattat tactcagcgg atatggcgtt ttgattggaa
      841 gcaaagagaa tgccgcggga ttaggtttgc aatgtaccta tctgaccgcc agaggcacca
      901 gcacggtaca atacctgcac actaaaagtg gttttctggg gataacagat tgcccgttgt
      961 tacgtaaaag caatattgtg gtcgataatg gttaagggta ataaataacc agattctcac
     1021 tcatgagtaa aatattctta tcgcaagtgc gtttaatata acgccacctt caactttatt
     1081 tttctcatgc taaataaaga acctgtctgt ttgatattca gacaggttct tactctcatc
     1141 agaacggata gtcgtgataa cccatttggg cagaaatttt gcgcgccgcg gtatgcaaca
     1201 ttgcgacata ttcctgtaaa cgctcttcag agaaacgtaa cgtcgggaag gagatgctca
     1261 aaccggcaat gaccacgcca aagcgatcaa ataccggaac cgcaatgcat cgcagccctt
     1321 cttcctgctc ttcgttatct tcgccatacc cctgctcgcg cacctggtcc agaacgggta
     1381 ataacgcttc tgtactggtg atggtccgct cggtactgcg tttatactcc acgccctcaa
     1441 gaatttgctt cacttcatcg cgatcgcgcc atgccagcag taccttacca atcgcggtgc
     1501 tgtacagcgg attacgacgc ccaatccgtg aatacatgcg caaattgtac atagagtcaa
     1561 ttttgtgaat gtaaacaata ctgtcttcgt ccagtgcgcc gaggtggata gtttctttgg
     1621 tcaggcggga gatctcacgc atctggatat ctgcgctacg aattaaatcg acgttttgta
     1681 acgcgcgagc gcccagttca aacaatttca gggtcagcga atatttctcc gactcccctt
     1741 cctgcgccac ataacctaag gttttcatgg tctgtaaaaa gcgataaacg gtgctttttg
     1801 acatcatgac gcgctgcgac aactcggtta tccctatttc gcgctcttca cccagcgcct
     1861 gcaaaatgcc aaaaactttt agcacggaag atacagaatc aggctgttta tccagatctg
     1921 cgttagccat ttatcacctc attgcgagtg ttttataaaa atcagaactg ttt
//
LOCUS       DAZUCC010000411         1947 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZUCC010000411 DAZUCC010000000
VERSION     DAZUCC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.413"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            97..768
                     /locus_tag="ACHY09_005157"
     CDS             97..768
                     /locus_tag="ACHY09_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3887971.1"
                     /translation="MKHAVKVMDEIAAQIQESGSLLGMIYSYMSENGAESERVYNAIR
                     CLMRSLQKTEETASIFAEKLCEAKAMVSHGHFFGKIRAQREACGLTTTELAKLLDLDE
                     EIILQWESGEFEPTISMLIPLANVIGCDPLSLLDEKDSASVIRVNAPEVYVESIGARI
                     KSARKKLGLTEADLARMINTYSDPINDWECGICEVPADQIVPLASALNCDLMWLLTGK
                     SEAKE"
     gene            774..974
                     /locus_tag="ACHY09_005158"
     CDS             774..974
                     /locus_tag="ACHY09_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887972.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHDLLCAVVVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            1173..1355
                     /locus_tag="ACHY09_005159"
     CDS             1173..1355
                     /locus_tag="ACHY09_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887973.1"
                     /translation="MHQKTAEHEQTRVLLTIKNGKVILIRHVHDDEFVGNLSTFLFIS
                     EKAGYDVIAPADEDEE"
     gene            1361..1933
                     /locus_tag="ACHY09_005160"
     CDS             1361..1933
                     /locus_tag="ACHY09_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887974.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPSPIRFALQQYIEWNLIQTYGEAEGMGRALFLYQKMLTTSPDKGF
                     ILSDIGREGLELLLDEMINDLNTHGMPEGQ"
BASE COUNT          627 a          395 c          439 g          486 t
ORIGIN      
        1 cataatctgt tctttgctgc ccaaaatgca tagctatagc gattgacata accaaacgct
       61 ttggctattg taaaattaaa acaacaggag gctcatatga agcatgcagt taaggtgatg
      121 gatgaaattg cagcacaaat acaagaaagc ggctcgctgc ttggaatgat ctattcctac
      181 atgtcagaaa acggagcaga aagtgagagg gtttataatg caatcagatg cctgatgcgt
      241 tccctgcaaa aaacagaaga aactgcatca atttttgcag aaaaactatg tgaggcaaaa
      301 gctatggttt cgcatggtca cttttttgga aaaatccgtg cccaacgtga agcctgcggc
      361 ttgacgacca ccgaacttgc caaactgctt gatctcgatg aagagatcat cctgcaatgg
      421 gagagcggag agttcgaacc gaccatcagc atgcttatcc ccctagcaaa cgtcattggg
      481 tgcgatccgc tttctttgct ggatgaaaaa gatagcgcgt ctgttattcg cgtaaatgcg
      541 cctgaagtct acgtggaaag tattggcgca cgcatcaaaa gcgcacgtaa aaaactgggc
      601 ttaacggaag ctgatcttgc ccgcatgatt aatacctata gcgaccccat aaacgactgg
      661 gaatgcggca tctgtgaagt tcctgctgat cagatagtgc cactggccag tgcgcttaat
      721 tgtgacctga tgtggttgtt aacgggaaaa tcagaagcaa aggagtagca acaatgactg
      781 gtaatatcca tgataagtat gaaggcttat gcctagcacc agattccttc gcaaacaata
      841 tccatgattt attatgcgca gttgtcgtat tacaaatgtc agacaacgac gcaataaaaa
      901 gaacaggtga tgaagttctt gaatttgcac gttgctatgc tgaagcagca gctgaaaaag
      961 aactatccag ttaaatagaa caagtcatct ccagatacta tattacggct taatcgccgg
     1021 ggattatcac acacttaatc cacaggaggt tttattatga cttttataaa acataaggca
     1081 tcacacaaaa cagcctgcct tattgcacaa cacgggaaaa attacatgca tattgcctgc
     1141 ttgtttctgc gtaaggcata cgggagataa taatgcatca gaaaacagca gaacacgaac
     1201 aaaccagagt attgctgacc atcaaaaacg ggaaagtaat attaattcgt catgttcatg
     1261 acgatgaatt tgtaggaaat ctttcaacat tcctgtttat ttcagaaaag gcaggatatg
     1321 acgttattgc accagcagat gaagatgagg aataaatttc atgcaatacg ctgaattcca
     1381 ggctgaagca acagccaatg gtatacaaac aggcagtatg actattgatt atcacgacgc
     1441 catacgccgt ctggatgctg gagaattcga tactcctaat gtgcgaggtt tacgtatcct
     1501 tcagtgtctc gcgcaagctg acgaagcagg attactggga aaacttccgg ttgagatgaa
     1561 ggttgcccag tggcgatggt tgtatgtgac gacattcatc aacgaagaag aagacaagaa
     1621 cggcacaatt gatgtcccga atgaacacgg aacaacagat cgcgccgtaa tatataacgg
     1681 gaagcatgga gttatgacta tataccctag tcccattcgg tttgccttac agcagtatat
     1741 tgaatggaat ttaattcaaa catatggcga agctgaggga atgggaagag cgctgtttct
     1801 ttatcagaaa atgctcacca cttcccctga taaaggtttc atcctttcag atataggtcg
     1861 agaagggctt gaactccttc tggatgaaat gattaacgac ctgaatactc atggtatgcc
     1921 agaaggccag tgacacatta aatatta
//
LOCUS       DAZUCC010000412         1944 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZUCC010000412 DAZUCC010000000
VERSION     DAZUCC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.414"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(617..1708)
                     /locus_tag="ACHY09_005161"
                     /pseudo
     CDS             complement(617..1708)
                     /locus_tag="ACHY09_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
BASE COUNT          605 a          444 c          353 g          542 t
ORIGIN      
        1 taaagattct caccatcatt cgcagtgaat aatgccgcat ctcatattca tatcctgcag
       61 agaaaacccc tccgaagagg ggcgagtgta gcgttaatgg agtgttatgt agcatggtgc
      121 cgggtgcctc ccggtgaaca gactgttcgt gataccagtc agcaacatag gaaattaatg
      181 gtatcacccc accgcacagg gggattcacc atgcctgcct tttttagcaa attcatcaga
      241 aaccggacaa catgcttatt tatgaaatga ggtgcttttc acatttatat aaaatcatta
      301 taacgataat cctaaaaatc aatgtaatta tcatcttcag taactttctg tattacacca
      361 tacggaaatt gatttttgac ggtaaagata caaggggaag tatgtctgta aggagtaccc
      421 tgaataatgg acaggcatgc atcgtggctg ttcccctgaa attatcagta caaccagtca
      481 gctcataatc cctgtctctt tttccgggga gccctcaggg ggactttccc ttatcccccc
      541 agtttctgga gagacgtaaa aaagaattaa tcatatgtca ggtatctgaa tatgtcccgt
      601 aattaatata tttacgtcag gattttttct gcccgccagg agtatgaaca tcttccgccg
      661 ttgagtctgt catatccatt tttttctgtg ccagtacttc atatgatttt tctttttcca
      721 gactctcctg ctgcatcccg tgagccggag catatacaag ctcagccatc actggaaaat
      781 gatctgaacc catatccggt aaacggcgta aacttttaag cagaaaatgc tggctgtgaa
      841 atacatggtc aagcggccat cgcaggaaaa aataatggct gtgaaacgtg ctaaaaaaac
      901 cacgtccccg gcgtggatcc agcagtccac ttattttcat gaataaacga gtggtcgcag
      961 accacgcaac atcattcaga tcccccgcta atattacagg taattcagaa tctgcaacac
     1021 ttttcccgac cattaaaagt tctgagtcac gatcagttga ctcatcactt tcagtcgggc
     1081 tcggtggaac aggatgaaga caatgtaatc ttattctctg accttccggc atatgaacta
     1141 tcatatgcat ggagggaatt tccttgtcca ctaaatactg tatttctgca tcacttaaaa
     1201 tcatccttga atatacatgc atgccatata aattttcttt cgggcatttc agggagtatg
     1261 gaaacacttc atccagaggt gatagtttct gttcccacca taagtttgtt tcaagaagaa
     1321 caagtatatc aggtttttct ttatttataa gttcaagcaa aggcatatat tttttattgg
     1381 tcataaggac attgaatacc agtatactta aacccggaaa tccatcagag tcctttattt
     1441 actttacctg cttcttgcca attactgtat aaggaattat ccataccaac tggtaagcga
     1501 cacaaattaa acttattatc acaacaaaca actctgcaaa tgagtcaaga aaaacaacag
     1561 acagaaaaac attcaaagta cacagcaaaa gtatctgtag tcggggaaaa tcccataccc
     1621 tgacaaccca tgatgtatta ccggaaacag ggataaaagt tatgactgcc agaaggatag
     1681 cagtaaaaat aaaaacacaa gttatcacaa atcactcctt gttctgaacc ggcacacaaa
     1741 actgtcatat acgtttcaaa agtaaaaaca cactgctgcc acaagattca cgacgtaacc
     1801 gggcagcata tcctgattac ggacaatcca tgaaaccgcc tcgccagaag cgtccatcac
     1861 atccggtttt tccctgtttt atattccacg aaccatttta ttttcaggaa tctccgggcc
     1921 tttatcccgc atcatataca cagc
//
LOCUS       DAZUCC010000413         1942 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZUCC010000413 DAZUCC010000000
VERSION     DAZUCC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.415"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            85..1161
                     /locus_tag="ACHY09_005162"
                     /pseudo
     CDS             85..1161
                     /locus_tag="ACHY09_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            1177..1809
                     /locus_tag="ACHY09_005163"
     CDS             1177..1809
                     /locus_tag="ACHY09_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFS3887975.1"
                     /translation="MEMENTPGRARTPWTIEDVRYLETHYGVKKTREIAAYLNRSATA
                     VRAMAYLVGITTPVAADWSETEIEIIRRDYPAGVALADICARLNGRAPKSVCWMARRF
                     GLKRLGYWSAEEDEILRQYYPSEGVKVSRRLSGRTENAVKLRARELGVKFTGNGRLRL
                     WTAEEWNVLSGNMASGLSALMKLLPGRSRSSVQGAMRRLQKMSAQKSVRQ"
BASE COUNT          531 a          466 c          545 g          400 t
ORIGIN      
        1 ttccaaccga gttgatgaac tcctgccatg gaacgtagta ctcaccaata aataagcgtc
       61 aatacggtgc tccgttgacg cttactgaat acccacgccc ggctggaaat tattaccggt
      121 ctgttcagtc gtctgcaaaa aacgctccgg gccatgccac cggatgagcg tgacaacctg
      181 cccctgctgt atcagacaga aacatgcctg aaaacgctga gtgacatact ggcccggcat
      241 gaacttaaac agagcagtaa agtggcttta ccggaagcca tggtgaaggg atatatcacc
      301 cgtctggaga atcagcctgt gcagggtgaa gtatcgtcgc cggtaaccct tccggctcag
      361 gcactccggt ctgatgctcc ggatgtacag gaacatcagc ccctgccgta ctcccgtctg
      421 gtgtatgttg tttctgaaac aaaaacagag cccacactct tccttcaaaa atccccgctc
      481 acgtttttga aaacgtttgt tacaggtgtc tgtacggcat tgctggcggt ctgcgtggcc
      541 attccgggat ggcagtttct gacacagcca tcaccggcgg agcagcaact cagggcgctg
      601 ctggcaccac taccggatgt gctttcagca gaccagatgg cgcaactcag cagaacaccc
      661 tcactgctaa atcatacgtc agagtggata accctttccg gtcagcagat aacgcgactg
      721 acagaactac cgccggcata caacctgcaa cgcagtgcgc agctgctgca gcaactgagg
      781 ggacttttcc ccgacaatcc ccgtgttcag gaaatggtgg ataactggca taagagcgta
      841 aggagccgcg cgctcccgga agaggcgatg acgggctgga acgaaggcat ggcccgttta
      901 cagcaactgg ctgagcgcct gaaccgtctg gatgaacagc ggggaaaata catgacggtc
      961 agtgaactga aaacggaggt gtttggcatc atgcaggctt ttaaccggca cataccggca
     1021 gaagagcagt tacgtcgtta tgatgaagcc cgtaaccaga atggcagtga acagcagcaa
     1081 aaacaggctg aaatggcgct gaatcagttg attaatcgtt atcagatgga gaacgcaggc
     1141 aaaccgaaga gccagccata atggctgaga taaaacatgg aaatggaaaa tacccccgga
     1201 agagcgcgca caccatggac aatcgaagat gttcgctacc ttgaaacgca ttatggcgtg
     1261 aagaaaaccc gcgaaatagc ggcatacctg aacagaagtg cgacagcagt tcgtgccatg
     1321 gcgtatctgg tggggataac aacaccagtg gctgcggact ggtcagagac agagattgag
     1381 attattcgca gggactatcc ggcaggcgtt gcgcttgctg atatttgtgc ccgtctgaac
     1441 ggacgagcgc cgaaatccgt gtgctggatg gcccgcaggt ttggtctgaa acgcctgggt
     1501 tactggagtg cagaagaaga tgaaatcctg cggcaatatt atccttcaga aggtgtgaaa
     1561 gtgagccgac gcctttccgg acgtacagaa aatgcagtga aactgagagc gcgggagctg
     1621 ggtgtaaagt tcacgggaaa cgggcgatta cgtctctgga cggcagagga gtggaatgtg
     1681 ttgtccggaa atatggcatc ggggctatcg gcactgatga agttactgcc ggggcgttcc
     1741 cgtagttcag ttcagggggc tatgaggcgt ttacagaaaa tgagtgcgca gaaatctgtc
     1801 aggcagtagc caaaatattc ctgcatcagg agaacggcag tggcaataat aagcagcaaa
     1861 ctgatacata atgcagcttc tgtaaacggt aatgatcaag tagggatatt tttatgttga
     1921 ctggttgtga aaaaatgatc aa
//
LOCUS       DAZUCC010000414         1932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZUCC010000414 DAZUCC010000000
VERSION     DAZUCC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.416"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            75..578
                     /locus_tag="ACHY09_005164"
     CDS             75..578
                     /locus_tag="ACHY09_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFS3887976.1"
                     /translation="MIMKNLAHVALITVIQLIACNLADWGGAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLFSPLLSLMTFIIGTLYELRRVCGCV
                     SVREWMQSQVNENTNEELHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            610..1341
                     /gene="traT"
                     /locus_tag="ACHY09_005165"
     CDS             610..1341
                     /gene="traT"
                     /locus_tag="ACHY09_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFS3887977.1"
                     /translation="MKTKKLMMVTLVSSTLVLSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQTWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSKVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            1401..1661
                     /locus_tag="ACHY09_005166"
     CDS             1401..1661
                     /locus_tag="ACHY09_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887978.1"
                     /translation="MNRAESRLSQVLFLLGYDSEKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
BASE COUNT          564 a          391 c          482 g          495 t
ORIGIN      
        1 acagcccaca ggaacttatg aacagggcaa aagagtatca ggataagtat aagcaataat
       61 gctgggtttg ggagatgata atgaaaaatt tagctcatgt tgctctgata actgtgatac
      121 agctcattgc ctgtaatctg gctgattggg ggggcgctga gacagttttt atcctgtgct
      181 ttattgtact atggcaggga ttgtttatat ggttgttttc tcaaatacga aaaaaacggc
      241 atgtgtcgga tgagtttaaa ttcagtaaag gcgtttggta tatactgatg ccagtatctt
      301 ctttattctc tccgttatta agcctgatga cttttatcat tggtacacta tacgaattaa
      361 ggcgagtttg tggttgtgta agtgtaagag aatggatgca atctcaggta aatgaaaaca
      421 ccaatgaaga attgcatctg gattttgatg atgtgaattt tgattatgtg gatttttatc
      481 gtactaatcc ggcaacagga ttagctatgc atggaggatt tgattcagca ggaaatactt
      541 ttgggacttg ttggcaggac tatgatgata ggcgataaga aaactgtagc ttgtaataaa
      601 ggtaatttga tgaaaacaaa aaaattgatg atggttaccc tggtcagttc cactctggtc
      661 ctttcagggt gtggtgcgat gagcacagca atcaagaagc gtaatcttga agtcaaaact
      721 cagatgagtg aaaccatctg gctggagcct tccagtgaac gcaccgtttt cctgcaaatc
      781 aaaaacacat ctgacaagga tatgagtgga ttgcaggcaa aaatcagtga tgccgttaaa
      841 gccaaaggct accaggttgt gacctcacct gacaaggcat actactggat ccaggccaat
      901 gtcctgaagg cagacaaaat ggatttgcgg gagtcccaga cctggctgaa cagcggttat
      961 gaaggtgcca ttgccggtgc agcattgggc accggcatca caggatataa ctccagctct
     1021 gccggtgcgg cgctgggtgt agggattgcc acagggttga ttggtatggc tgccgatgca
     1081 atggtggagg atatcaacta caccatgatc acggatgtgc agattgcaga gcggacaaca
     1141 tctaaagtca gaactgataa tgttgctgct ctccgccagg gaacctccgg tgcaaaagtg
     1201 cagaccagca catcaaccgg aaatcaacat aaataccaga cccgtgtggt atctaatgcc
     1261 aacaaggtta acctgaaatt tgaagaagcg aagcctgtac tggaagacca gctggctaaa
     1321 tccatcgcga acatcctgta aattacccat ccggcccgga cagattgtac cgggccgata
     1381 ttcctgcccc ggatgaatac atgaaccgtg cagaatctcg tctctcgcag gtactctttc
     1441 tgctgggcta tgacagtgaa aagtatcctg agcatgtatg tcgggagtta ttaaaagaac
     1501 tttaccggtt tgctgatgca accggagatt ctgaactggc catagcctgc cagagatacc
     1561 tgacagcact ggaaccgttc tctgcatatg agatcggcgt aagcgcaaca gaatatcttc
     1621 ctgtgagtga atatccgacc ggacgtcgca gaagaaaata gctgacgcct cgcgttgctc
     1681 gttgtccaac cccggaaagt aacaaaataa attttgtcac ttcccggcgt ttccgaaata
     1741 aatatgagaa cactgaacgg ttgtgcaaat tctttgtgcg accgtgaagt gttgccggcg
     1801 aagccggaag cttccggaga aagtcattat gacatcagac aatatcgctg aatatgttgg
     1861 tgagaataat caatttactg agaagcctgt aactgaatac tccccggtga tacaggataa
     1921 tacagcgacc ac
//
LOCUS       DAZUCC010000415         1870 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZUCC010000415 DAZUCC010000000
VERSION     DAZUCC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.417"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..133
                     /locus_tag="ACHY09_005167"
     CDS             <1..133
                     /locus_tag="ACHY09_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFS3887979.1"
                     /translation="SSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(803..892)
                     /locus_tag="ACHY09_005168"
     tRNA            complement(803..892)
                     /locus_tag="ACHY09_005168"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(856..858),aa:Ser,seq:cga)
     gene            986..1783
                     /gene="mtfA"
                     /locus_tag="ACHY09_005169"
     CDS             986..1783
                     /gene="mtfA"
                     /locus_tag="ACHY09_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFS3887980.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
BASE COUNT          518 a          389 c          414 g          549 t
ORIGIN      
        1 atcatctcat tttcacattt ttatgggtaa tgattcccag caatctttat tgaatgaaat
       61 ggaaaactgg ccaacgtatt atccctatca gttgagtagc gaagaagtgg tcgaggagat
      121 gatgtctcat tgattttttt gcgctgactt cagttattta acagaacagt gcctggtagt
      181 aacaacaata atttaccaag acaaataagt aagacaaact cagacacgct catacttgag
      241 tcgtgtcttt tttattatgt ctgtagaaaa aaactgcgaa aaaggcgaac aataaataca
      301 caactcagcc atacagactg gcagcgcaaa tgtttccagg agcaagtcat ctgtcatggt
      361 aaagctaacc agaaatatag gttaattttg gtgtttaaac cttttttact tgttagtatg
      421 atacaaaggc tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt
      481 cccgcggctt taatctgacg gccgcgttcc ttttttggct caattattga ccccttctct
      541 acgcaacttc agttcccgcc agcaactttg cggcagtttc ataggatcaa tgtctaaaag
      601 aataatggtg atcgataaga aaacgactga ataactgtag attttcgctc gaaaccttcc
      661 tgtcagctcc atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg
      721 atctaaccct tcaagaatat tctacgattg ttctgtttag gaaaagcaag gcgggaagtc
      781 gggagataag tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg
      841 cccctactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
      901 ggttgccatg atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc
      961 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     1021 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac
     1081 ggaacaggaa caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct
     1141 tgttccttta cagggctttg aactggattc attaagaagc tgccggatag cacttctatt
     1201 ttgcctgccc gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat
     1261 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     1321 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     1381 ggatatacaa gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca
     1441 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattt cgttgcgtga
     1501 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     1561 attagttggt gagaatgcgg cgagcattga tgcttatgct gccagtgatc ctgctgaatg
     1621 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     1681 ttcattgtgg caacgtttct gtcaatttta tcaacaagat cctttgcaga gactgcatca
     1741 cgctaatgat acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat
     1801 taattaacca attgaaatgg cttatgaaat ttagtgttga cagacaaggt accgctaagt
     1861 aatatgcgcc
//
LOCUS       DAZUCC010000416         1867 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZUCC010000416 DAZUCC010000000
VERSION     DAZUCC010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.418"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..92
                     /locus_tag="ACHY09_005170"
     CDS             <1..92
                     /locus_tag="ACHY09_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFS3887981.1"
                     /translation="VYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(476..1042)
                     /locus_tag="ACHY09_005171"
     CDS             complement(476..1042)
                     /locus_tag="ACHY09_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887982.1"
                     /translation="MDEGKQPGTPGMNDIISIIILLTVYLIGLIFIILTINDIDRSSK
                     KRKYEKKLKEAIINGSLQNNDICLLAERWSASHEHIKSALLSVFNDFLTDNNFSHHVP
                     RVRELITWYEKYAPFTELPEDIKQQLQNIISLPDVSESEVQLLATSLHDIYNSCQKKE
                     KLARRINYVLGAVSIASFCISLFQILFK"
     gene            1155..1496
                     /locus_tag="ACHY09_005172"
     CDS             1155..1496
                     /locus_tag="ACHY09_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFS3887983.1"
                     /translation="MFSFPASSCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
     gene            1496..1738
                     /locus_tag="ACHY09_005173"
     CDS             1496..1738
                     /locus_tag="ACHY09_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFS3887984.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
BASE COUNT          426 a          426 c          490 g          525 t
ORIGIN      
        1 cggtgtatcc gaacctgctg atatggccgg cggccatgct gctgggggtg gccatcaccg
       61 tgaccatggc atccttcaag aaatatttat aatccacaga ggccggacac cctgatgcaa
      121 aacttgtttt gtggcagggt gtcggcagac aagccacgcc cttacggtca gtgcgcgtca
      181 gtgtcagagt aacgacagcg ccggaatgac aacaatgttg acgaaaataa tgaacagagt
      241 gatggccctg atgacgtcat agatccacgg ggggcaacga ggttcttcat aaatgaaaaa
      301 atacacaaca caaacccagg tcagaaggaa cagggcccag aacggaaggg cagtacggag
      361 aaattccatg ctgatgtgtt tcctctttaa attgttaatg aggtgtcatt aacgggaaac
      421 agtagtgatg ccgtgtttta ataaacagga ataaatcatt caggctggaa taaatttatt
      481 tgaacagaat ctggaacagc gagatgcaga aactggctat gctgacagcc cccagaacat
      541 agtttattct tctggccagc ttctcttttt tctgacagga attataaatg tcatgaagtg
      601 acgtggctaa taactgcact tctgactcac tgacatcggg taaactgatt atattttgta
      661 actgttgttt tatatcctca gggagttctg tgaatggggc atatttctcg taccatgtga
      721 tcagttctct gacccggggg acgtgatgtg aaaagttgtt atcagtcaga aaatcattga
      781 aaacagacag cagtgcgctt ttgatatgtt catgactggc agaccagcgt tcggccagta
      841 aacagatatc attattttgt aaactgccat ttattatcgc ttctttcagt tttttctcgt
      901 actttctctt ttttgatgat ctgtctatat cattgatagt caggataatg aatatcaggc
      961 cgatgagata gactgtcagg agaatgatta tactgatgat gtcgttcatg ccaggagttc
     1021 cgggttgttt gccttcatcc acaccagtgt attctcccgt aaaaattttc tgttttgcaa
     1081 atagagttta tcgctgatcc agtctcctgc cttacgcatc ctgtacgcac tgtttaactc
     1141 tgacggaggt gcgtatgttt tcttttccgg catcatcctg ccgtgccggc cgtctggccg
     1201 gtgccgcggg gatgctgttt cttttgtcct ttgcattacc tgcgcaggct gccgaaaagg
     1261 atgaactggc cactgtaatg cgccagcttg accacgtgca ggcagcgctg gagcgggcca
     1321 gaacggcctc tctgcaggct tcttcacagg gggaacgcta cttttttgat tatttgcgtg
     1381 cccgtgcaga tctgcagacc attcgtgacg gtattgacca ttatctggcc ccttcccggg
     1441 cgcaaccccg gtcttccgca gccgtgaccg gtgactaccg gcgggagcgt ccctgatggc
     1501 gatgacggcc ctgcagaata cggccttccg ggctgcttca ggtgaagtgg atctcagtgt
     1561 gctgcatctc atcacgctgg gatttctgct ggcagtcgtg ttcctctggg cagcgtggag
     1621 tctgacggat gcctgggcag gctggcgtag cggtgatgtc cgggatggtg tgttcggctg
     1681 gtttatgttc cgactcgtcc tgctgctgat ctgcagtatc tggatgtttg cgggttgatt
     1741 tcagtgtctt gtttaagttt caggaggaaa taatgcataa aaaaatgcgc atctgtcgcc
     1801 aggcctggta tgccatggcg gtgtttctgt gtaccgggat cacacctgct ctggctgatt
     1861 tgcctca
//
LOCUS       DAZUCC010000417         1846 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZUCC010000417 DAZUCC010000000
VERSION     DAZUCC010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.419"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..848
                     /gene="rcnA"
                     /locus_tag="ACHY09_005174"
     CDS             24..848
                     /gene="rcnA"
                     /locus_tag="ACHY09_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFS3887985.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGKRNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1067..1405
                     /gene="rcnB"
                     /locus_tag="ACHY09_005175"
     CDS             1067..1405
                     /gene="rcnB"
                     /locus_tag="ACHY09_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFS3887986.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPDMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1487..>1846)
                     /locus_tag="ACHY09_005176"
     CDS             complement(1487..>1846)
                     /locus_tag="ACHY09_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3887987.1"
                     /translation="KGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAED
                     NGVGFMLESNSSPVTFLNITDNSKGYTNLKEIAAKSKITDTTVSIPVTASYYVYDKNK
                     VKSGALQATALINVKYD"
BASE COUNT          469 a          437 c          406 g          534 t
ORIGIN      
        1 aattaatact aagaattatt atcatgaccg aatttacaac acttcttcag caaggaaacg
       61 cctggttctt catccccagc gccatcttac ttggtgcgct tcatggcctg gaaccggggc
      121 actcaaaaac gatgatggcg gcgtttatca tcgccatcaa aggcaccatt aaacaggcgg
      181 tgatgctcgg actggcagca actatttcgc ataccgcagt agtctggtta attgcctttg
      241 gcgggatggt gatcagcaaa cgctttactg ctcaatcagc agaaccgtgg ctccagttga
      301 tttccgcagt gatcattatt agcaccgcgt tctggatgtt ctggcgtacc tggcgcggca
      361 aacgcaactg gctggagaat atgcacgagc atgatcatga gcatcatcac cacgatcacg
      421 aagatcacca cgaccatgga catcatcacc atcacgaaca tggcgagtat caggatgccc
      481 atgcacgagc ccatgccaat gacattaaac gacgctttga tggtagagag gtcaccaact
      541 ggcaaatttt gttatttggc ttaaccggtg gccttatccc ctgcccggca gcaattaccg
      601 tgctgttgat ttgcattcag ttgaaagccc tgacactggg cgcaacactg gtcgtcagtt
      661 tcagcatcgg cctggcgtta acgcttgtca ccgtaggcgt tggcgcagca atcagcgttc
      721 agcaggtcgc aaaacgctgg agcggattta acactctcgc taaacgcgct ccctattttt
      781 ccagtctgtt gattggctta gttggtgtgt atatgggcgt acatggcttc atgggcataa
      841 tgcgataact caatccttta ggcttgcatg ttatcttcag cccaggatga ttcttaaatc
      901 agctatttcc gctgactctc tgctcgctaa ggatgctttt agggcatcct ttcatttaca
      961 ctttttacga aatcatggga tcactaacaa aatatcgctt gtcagttata ttgtatggca
     1021 ggaaagatat gcgactgata ttacagatcc ccaaagtgga gagtttatga ccattaaaaa
     1081 taagatgttg ctgggtgcgc ttttgctggt taccagtgcc gcctgggccg caccagccac
     1141 cgcgggttcg accaatacct cgggaatttc taagtatgag ttaagcagtt tcattgctga
     1201 ctttaagcat ttcaaaccag gcgataccgt cccagacatg taccgcaccg atgagtacaa
     1261 cattaagcag tggcagttac gtaacctgcc cgcgcctgat gccgggacgc actggaccta
     1321 tatgggtggc gcgtacgtgt tgatcagcga caccgacggt aaaatcatta aagcctacga
     1381 cggtgagatt ttttatcatc gctaaaaaaa gccccctcat catgaggggg aaatgcagac
     1441 accttgttat tttttattat tcgccacttg ctcgtcttgc ttattattag tcgtatttca
     1501 cattgattaa tgcggttgcc tgtagtgcac cagattttac tttattttta tcataaacat
     1561 agtaactggc agttactgga attgagaccg ttgtatctgt aatttttgac ttcgccgcaa
     1621 tttcttttaa atttgtgtac cctttgctgt tatcagtgat atttaaaaat gtgactggcg
     1681 aactattgct ttcaagcata aatccgactc cattatcttc tgcacctttc aggatcgtgt
     1741 tactgccggc taacaaatca gaacttgata aatacacctg catttttcgt gatgtttgat
     1801 tttgttgacc taataaattt ttacaacgca gaataatatc cccttt
//
LOCUS       DAZUCC010000418         1843 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZUCC010000418 DAZUCC010000000
VERSION     DAZUCC010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.420"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..335
                     /gene="bssS"
                     /locus_tag="ACHY09_005177"
     CDS             81..335
                     /gene="bssS"
                     /locus_tag="ACHY09_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFS3887988.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            450..1568
                     /gene="solA"
                     /locus_tag="ACHY09_005178"
     CDS             450..1568
                     /gene="solA"
                     /locus_tag="ACHY09_005178"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFS3887989.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1616..1729
                     /locus_tag="ACHY09_005179"
     CDS             1616..1729
                     /locus_tag="ACHY09_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFS3887990.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVYYF"
BASE COUNT          479 a          446 c          442 g          476 t
ORIGIN      
        1 tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta
       61 acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg
      121 tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc
      181 agaccccaaa taagtccgag caggaaggga ctgaagttgg tcagacgctc tggttaacca
      241 ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg
      301 gtgatttccc ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag
      361 gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct
      421 gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg
      481 gttccgtagg cgctgccgcc gggtattatg caacccgtgc cggtttaaac gtgctaatga
      541 ccgacgccca tatgccaccg catcaacacg gcagccacca cggcgatacg cgattaattc
      601 gccatgctta tggtgaaggc gaaaagtatg tcccgctggt tctccgcgcg caaacgctgt
      661 gggatgaact ctcccgccac aacgaagaag atcccatttt tgtacgctct ggcgtcatta
      721 accttggccc ggctgattcc acatttctcg ccaacgtcgc ccacagcgcc gaacaatggc
      781 aactcaacgt tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg
      841 tcccggacaa ctacatcggc ttatttgaga ctgattccgg ctttttacgc agcgaactgg
      901 cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcagctg ttcaactgcc
      961 cggtcaccgc aattcgtcat gacgatgatg gcgtaactat tgaaacggct gacggtgagt
     1021 atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagt
     1081 tgcctgtcca gcccgtacgc aaagtatttg cctggtatca ggccgatggg cgctatagcg
     1141 tgaagaataa attcccggcg tttaccggtg aacttcccaa tggcgatcaa tattatggtt
     1201 ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt
     1261 cagcggatga acgtgttccg tttgcggaag tggtcagcga tggttcggaa gccttcccgt
     1321 tcttgcgcaa tgtattgccg ggtatcggtt gctgcctgta cggcgccgcc tgcacctatg
     1381 ataattcgcc tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc
     1441 tcattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg
     1501 ccgattttgc gcaagacaaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct
     1561 tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg
     1621 tcggctgttg cactatctca tcaataatat tcgcgagcat ctgatgcttt atctttttct
     1681 ctggggattg ttggcaatta tggatctgat ctatgtgtat tatttttagt tttccgcact
     1741 gacataaact gacaaatatt taatctccct ttcatttagc gtgaaaaatt gaatgagatt
     1801 attcctgctt caacagcact caccagacat ctctgatgct gaa
//
LOCUS       DAZUCC010000419         1836 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZUCC010000419 DAZUCC010000000
VERSION     DAZUCC010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.421"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..193)
                     /locus_tag="ACHY09_005180"
     CDS             complement(5..193)
                     /locus_tag="ACHY09_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFS3887991.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            complement(268..783)
                     /locus_tag="ACHY09_005181"
     CDS             complement(268..783)
                     /locus_tag="ACHY09_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFS3887992.1"
                     /translation="MRFICDNSYFLEGVKEYINPGKFIISGMQTDNVVIISSSRISEI
                     VGFLYKNNISDMNTLYFSSPEALRFLWGVVDSPVYDIRHLTERCSVNDISHLYRAFMS
                     VEKLTLSGRQVNVLMMLLYGSNGTEGARYLGMSEKTFSTHKQNLIKRLRVHNEVELLY
                     KGMLLVRKGRL"
     gene            complement(907..1383)
                     /locus_tag="ACHY09_005182"
     CDS             complement(907..1383)
                     /locus_tag="ACHY09_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFS3887993.1"
                     /translation="MQIIIMTRDRYLEYGLMRMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVIFHEYGCTFLADQLGISMKTVCAHLYNAMEKSGLRGVSVKYLCSTADR"
     gene            complement(1446..1652)
                     /locus_tag="ACHY09_005183"
     CDS             complement(1446..1652)
                     /locus_tag="ACHY09_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3887994.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
BASE COUNT          519 a          530 c          357 g          430 t
ORIGIN      
        1 gtcatcattt ccccgtggct tcgcggattt tctgttccgt cttatactgc gaaagcgcat
       61 acacggccca tattgttgcc ggtatccagc ccagcagcgt tatctgtaac accagacaaa
      121 aaatccccgt cagaggccgg ccgatggtga aaaaagtcag ccagggaaga agaaaagcaa
      181 tcaacagacg cataaaaact ccttatgatt atgaaattaa cgaatcagag gctgcgttta
      241 acattaccgg aaataagctg tcctgcttca caacctccct tttctgacca gaagcatccc
      301 cttgtacaac aactcaactt cattatgtac ccgtaatctc tttatcagat tctgcttatg
      361 agtggagaat gttttctctg acatgccaag atatcgtgcc ccctctgttc cgttactgcc
      421 ataaagcagc atcatcagta catttacctg ccggcctgac agggtcagct tctccaccga
      481 catgaatgcg cggtacagat gactgatatc gtttactgaa catcgctcag taagatgtct
      541 gatatcataa accggactgt caaccactcc ccagagaaaa cggagcgctt ccggagaaga
      601 aaaataaagg gtgttcatat cacttatatt attcttatac agaaagccga caatttcaga
      661 tatccgggaa gaagatatta tcaccacatt atctgtctgc atgccgctga taatgaattt
      721 accgggatta atatactcct tcactccctc cagaaaataa ctgttatcac aaataaaacg
      781 catacccgtt ccacatgaaa tttatcacgc ctgaccacat aacattaatt atccggagca
      841 ccgggatgcc cgtgacacag catgcacaga aattcccgtc gcaacagcct tcatcagacc
      901 ggctttttac cggtctgcgg tgctgcagag atatttaaca ctgacacctc gcagaccgct
      961 tttctccatc gcattgtaaa gatgcgcaca taccgtcttc atgcttatcc ccagctggtc
     1021 tgccagaaag gtacagccgt actcatggaa aataaccccg aagaccacca ccatctcggt
     1081 acgggtcagt ggtcttgccg tatgtccgaa cagccacgca ccacgacgga tggcctgcct
     1141 gatatctgtc aggcatctca cagaggaaac aggaatgaca agaaaacgac gcccacagaa
     1201 catacagtat gtaagccttt ccagattacg gtcacagaga atcacataac tgtcttcggg
     1261 aagtggcaga cggcgctttc cggcatcaaa cagttcacgg cctgtcgtga gcctgtatcc
     1321 gttcagcata cgcatcaggc catattccag atatctgtca cgggtcataa taataatctg
     1381 catactctca gacccttatc tcccggatac cggctccgtt caccattacc ggtataactg
     1441 actcatcatt cataacggaa actgtctgcc gcatacatca gtgcccgctc ttccagctct
     1501 gacagactca gctctttcag ttccccttct ccttcagcaa gaagacaaca ttcaaaaaca
     1561 gctgcatcgt cgcattccag acgaacctgc accctgtcag cccgaccgtt tacagtaaat
     1621 acctgcactg aacttatgcg atagttcagc atatacactc tccctctcag ctcctcagga
     1681 ataccccgga accgtcagtc atgccaccgg ggagaaaagc aggcagcgaa tcatgaccgc
     1741 atacagaacg ctgcccgccc cggtgactca gacccgggtt cagaactccc agttgacacc
     1801 cacattccag gaaacatcac cgtcactgaa cgcagt
//
LOCUS       DAZUCC010000420         1828 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZUCC010000420 DAZUCC010000000
VERSION     DAZUCC010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.422"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            75..368
                     /gene="iss"
                     /locus_tag="ACHY09_005184"
     CDS             75..368
                     /gene="iss"
                     /locus_tag="ACHY09_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFS3887995.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(459..641)
                     /gene="rzoD"
                     /locus_tag="ACHY09_005185"
     CDS             complement(459..641)
                     /gene="rzoD"
                     /locus_tag="ACHY09_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFS3887996.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(858..1355)
                     /gene="rrrD"
                     /locus_tag="ACHY09_005186"
     CDS             complement(858..1355)
                     /gene="rrrD"
                     /locus_tag="ACHY09_005186"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFS3887997.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(1355..1570)
                     /gene="essD"
                     /locus_tag="ACHY09_005187"
     CDS             complement(1355..1570)
                     /gene="essD"
                     /locus_tag="ACHY09_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFS3887998.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          485 a          434 c          410 g          499 t
ORIGIN      
        1 catcaatcca tttaactatc gtttaattgt tgtcacatag gattctgccg tttttaacaa
       61 tgcaggataa taagatgaaa aaaatgttgt tttctgccgc tctggcaatg cttattacag
      121 gatgtgctca acagacgttt actgttggaa acaaaccgac agcagtaaca ccaaaggaaa
      181 ccatcaccca tcatttcttc gtttcgggaa ttggacagga gaaaactgtt gatgcagcca
      241 aaatttgtgg cggcgcagaa aatgttgtta aaacagaaac ccagcaaaca ttcgtaaatg
      301 gatttctcgg ttttattact ttaggcattt atactccgct ggaagcgcgt gtgtattgct
      361 cacaataatt gcatgagttg cccatcgata tgggcagctc tatctgcact gctcattaat
      421 atacttctgg gttccttcca gttgtttttg catagtgatc agcctctctc tgagggtgaa
      481 ataatcccgt tcagcggtgt ctgccagtcg gggggaggct gcattatcca cgccggaggc
      541 ggtggtggct tcacgcactg actgacagac tgctttgatg tgcaaccgac gacgaccagc
      601 ggcaacatca tcacgcagag catcattttc agctttcgca tcagctaact ccttcgtgta
      661 ttttgcatcg agcgcagcaa catcacgctg acgcatctgc atgtcagtaa ttgccgcgtt
      721 cgccagcttc agttctctgg catttttgtc gcgctgggct ttgtaggtaa tggcgttatc
      781 acggtaatga ttaacagccc atgacaggca gacgatgatg cagataacca gagcggagat
      841 aatcgcggtt actctgttca ttgctgaccc cacaaacaga tttcacgctc aatctcacga
      901 cgagtcatga gacctttcca ttgcttaccg ccagcatatg tccagcgacg tagctgatca
      961 catgcgcctt tgatatcgcc ctggtttatt ttgcgaagaa gcgtcgatgt tctgaaattg
     1021 ccagcaccca cgttgtaaac gaatgagtaa agagcgccgc gcattgtttc cggtatatcg
     1081 actttgatgt acgggttaat ttgtctggcg accgtggcaa ggtctttatt caggagggct
     1141 ttgcattctg ctttggtata cgttttaccg agcatgatgt cttttcctgt atgcccgtga
     1201 catacagtcc atacaccaac aatatctttg tatggtatgt agctgacacc ttccagacca
     1261 tcgttaccac ttgggccagt gattaacact gatgctatag caattgctcc gccaccaata
     1321 gcagcagcaa cggcttttcg taatgatgga ggcattattc acctctcgca gccttgcgct
     1381 tatcttcttt aatcttgaaa taaaggtttg tcaggtacgt cagcaggcca aataccaggc
     1441 tacccagcac acctattgct gcccactgtg agggcgtgac tttatcgagc agctgtaaaa
     1501 accagtaacc ggcactacct gctgaggtgc cataggcgac acccgttgtt aacttatcca
     1561 tggatttcat aaccccacct cgcagacaaa gcgggtgtaa attgagggaa tacaacgtat
     1621 cgcaaaaaag cagaaacgta acagactcgg agtcagtgaa taactcaggt attgggttat
     1681 cagctaatat cgagactcaa aaaatggaaa aacccgctcg acggcgggtt taagctgtgt
     1741 gacgaagtaa ccactcttaa cagcataacc aattttttac gtacgtaaac cacaaaatga
     1801 tatttgcgag aatgctaccg agtattga
//
LOCUS       DAZUCC010000421         1821 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZUCC010000421 DAZUCC010000000
VERSION     DAZUCC010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.423"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..823
                     /gene="gadX"
                     /locus_tag="ACHY09_005188"
     CDS             <1..823
                     /gene="gadX"
                     /locus_tag="ACHY09_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFS3887999.1"
                     /translation="QPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQVD
                     KCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQN
                     KNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWTL
                     ARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGYH
                     SVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            1192..>1821
                     /gene="gadW"
                     /locus_tag="ACHY09_005189"
     CDS             1192..>1821
                     /gene="gadW"
                     /locus_tag="ACHY09_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFS3888000.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKC"
BASE COUNT          538 a          349 c          382 g          552 t
ORIGIN      
        1 gcaaccacta catggtaatt gtctaattgc gtacgcaaga cataaatata ttctcaccat
       61 ggttaatggt gaatatcgct attttaatgg cggtgatctg gtttttgcgg atgcaagcca
      121 aattcaagtg gataagtgtg ttgagaattt cgtattagtt tcaagagaca cgctttcatt
      181 atttctcccg atgctcaagg aggaggcatt aaaacttcat gtacataaaa aagttccttc
      241 attactcgtt catcactgta ccagagatat tcctgttttt caggaagttg cgcaactatc
      301 gcagaataag aatcttcgct atgcagaaat gctacgtaaa agggcattaa tctttgcgtt
      361 gttatctgtt tttcttgagg atacgcagtt tataccgtta cttctgaacg tgttgcaacc
      421 gaatatgcga acacgagttt gtacggtcat caataataat atcgcccatg agtggacatt
      481 agcccgaatt gctagcgaat tgttgatgag tccaagcctg ttaaagaaaa aattgcgcga
      541 agaaggtacg tcatattcac agttgcttac tgagtgtagg atgcagcgtg ctttacaact
      601 tattgttata tatggggttt caattaagcg agttgcagta tcctgtggat atcacagcgt
      661 gtcgtatttc atttacgttt ttcgaaatta ttatgggatg acgcccacag agtatcagga
      721 gcgatcggcg caggaattac cgaactgtgg ctcggcggca agtatggctg cgcaagggat
      781 tttttacggc actgaccgtt ctgcggaagg aataagatta tagagtttta ctcagacata
      841 aaaaaaaccc ggcatagggg accgggaaga ggatagtctg ccgtctccag actaataaac
      901 cgttataaca ctccctgttg gcacgggaaa ctttgtgctc tcagtaagtt aaatataact
      961 tttactggaa ataagatcag ccattttttt ataaacataa gctatacgct gtgtgaaaat
     1021 ataatcgtgt tgcatttact tattatcaat taactgttat gcaaaactac tttgtggata
     1081 aaatttggtc ctaccaaata tggcagtttt tgcacacaga aacagtctgg cgtcatttca
     1141 ttagtatact gacattgaaa taatcgcagt aatgaaatat aagggatagt catggctcat
     1201 gtctgctcgg tgatcctcgt tcgtcgttca ttcgatattc atcatgaaca gcaaaaaata
     1261 tcgttgcata acgagagtat cctgctgctg gataaaaatt tggcagacga ttttgcgttt
     1321 tgttcactgg atacgcgacg gctggatatc gaagagctga cagtttgcca ttacttacaa
     1381 aatattcgtc agttgccacg caatttagga ttgcatagca aagaccgttt gttaattaac
     1441 cagtcacccc ccatacagct ggtgacggcg atttttgata gtttcaatga cccccgggtc
     1501 aattcgccga tactgagcaa aatgctctat ctttcctgtt tatcaatgtt ttctcataag
     1561 aaagaactga tccccttact tttcaatagt atcagtactg tttcaggaaa agttgaacgc
     1621 cttattagct ttgatatcgc taaacgttgg tatctacgcg atatcgcaga aagaatgtac
     1681 accagcgaga gtctcatcaa aaaaaagttg caggatgaaa atacctgttt cagtaaaata
     1741 ttactcgcct ccaggatgtc gatggccaga cgattactcg agttacgtca aatacctctg
     1801 catactattg cggaaaaatg t
//
LOCUS       DAZUCC010000422         1813 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZUCC010000422 DAZUCC010000000
VERSION     DAZUCC010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.424"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..208)
                     /locus_tag="ACHY09_005190"
     CDS             complement(<1..208)
                     /locus_tag="ACHY09_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888001.1"
                     /translation="MRMFTNLLYDICTVFELFKEGESPRDKRKSTDFGAHQRFWDQRY
                     NELSHIIDAEGVYSLEQRRIIFSRY"
     gene            309..557
                     /locus_tag="ACHY09_005191"
     CDS             309..557
                     /locus_tag="ACHY09_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3888002.1"
                     /translation="MSTQTGHFYTPSRILRMAELTRLLGISRSTIYEKMNPASKYYDA
                     TFPRPVRLGSSAVGWRASAIDEWLTLHTTPTRSVTKDK"
     gene            573..>1813
                     /locus_tag="ACHY09_005192"
     CDS             573..>1813
                     /locus_tag="ACHY09_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFS3888003.1"
                     /translation="MTKLSPPELDITKLPDRCQALLDEMHEETGISREILLSIMLTVK
                     AASVQDTHEVELSGGQRTSLQIYMCLSSASGSGKTSACTKLIAPVHETEEELHQAYID
                     DKKNYDRMMEMWTTDKKILERRYKKEMERSPENAAAARAALEKCIANKPVPPVQQVLI
                     VNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVTVERASRP
                     GFRVKDSRLTMLILTQPVIFDKFFTLTGDQIRGNGFLARVLFCEPGANKIMTTEPHTA
                     TPVVTQQCATCFHEKSFGSQIRDSLRASRERRAKGEQRICMTLSNAASHDLEIFHKEN
                     MSAVRQNPRMTTFEDIIVRKREQAVRIAALLELENNPHGTVITRESINSAIYLVDFYF
                     QHLISKLESLREISPAEKLDK"
BASE COUNT          559 a          434 c          378 g          442 t
ORIGIN      
        1 cataccggga aaatataatt cgccgttgtt ccagactata caccccttca gcatcaataa
       61 tgtgactgag ctcattataa cgttgatccc agaatctttg atgcgctcca aaatcagtac
      121 tcttccgctt atctctcgga ctctctcctt ccttaaacaa ctcaaaaact gtacaaatat
      181 catacaaaag atttgtaaac atgcgcatct gctctgatct ccggttggtt tccgttttat
      241 cacaggggac attctggcct taacctagca aattaatgac atagccttca actcacatgg
      301 agaacaatat gtcaacccag accggacatt tttatacccc ctcacgcatc ctgcgtatgg
      361 ctgaacttac ccggctcctt ggtatatcaa gatcgaccat ctacgaaaaa atgaatccgg
      421 catcaaaata ctacgatgcc acctttcccc ggcctgtccg gcttgggagt tcggctgtag
      481 gctggcgggc atctgccatt gatgagtggt taactttaca tacaacacca accagatccg
      541 taactaagga taaataagga aaactgtacg ccatgacaaa attatccccc cctgaacttg
      601 acataactaa actgcctgac aggtgccagg cattacttga tgaaatgcat gaagaaacgg
      661 gaataagccg tgaaatactg ctgtctatta tgctgacggt caaggctgcc tcggtccagg
      721 atacgcatga agttgaactt tctggagggc agcgtaccag tcttcagata tatatgtgtc
      781 tttcatcagc atcaggcagt ggaaaaacct ctgcctgcac aaaattaatc gcccctgttc
      841 atgaaacaga agaagaacta catcaggcat atatcgacga taaaaaaaac tatgatcgca
      901 tgatggaaat gtggacaaca gataaaaaaa tcctggaacg gagatataaa aaagaaatgg
      961 aaagatcccc ggaaaatgct gctgctgcac gagcagcgct tgaaaagtgt atagcaaata
     1021 aacccgtacc tcccgtacaa caggttctca tcgtgaatga cgcgacacca gaagggatag
     1081 ctttgaaact cagccagtca ccttctcttc tgttgctgtc tgatgaagga ggaactattc
     1141 tggataagcg tttcgaacgc aaatcggcgc tgtacaacac attatggagt gggcaaccag
     1201 tcaccgtaga acgggcatcc agacccgggt tccgggtaaa ggattcccgt ctcactatgc
     1261 ttatactcac tcaaccggta atatttgata agttttttac tctcactggc gaccagatcc
     1321 ggggcaatgg ctttctggcc cgggtgctat tttgcgaacc cggagcaaat aaaataatga
     1381 cgacagaacc gcatactgcc actcctgttg taacgcagca atgtgcaacc tgttttcatg
     1441 agaaaagttt tggcagtcag atcagagact ctctgagagc gtcccgggaa aggcgtgcaa
     1501 aaggtgagca acgcatctgc atgacattat caaacgcagc atcccacgat ctggaaatct
     1561 ttcacaaaga aaatatgagt gcagtcaggc agaacccacg tatgaccact ttcgaagaca
     1621 ttatcgtcag gaaaagagaa caggctgttc gaatagccgc acttctggag ctggagaaca
     1681 atccacatgg tacagtaata acacgagaaa gtatcaatag tgctatttat ctcgttgatt
     1741 tttattttca gcatcttata tccaaactgg aatcacttcg ggaaatatct cctgcagaaa
     1801 aactggataa atg
//
LOCUS       DAZUCC010000423         1812 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZUCC010000423 DAZUCC010000000
VERSION     DAZUCC010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.425"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..866
                     /locus_tag="ACHY09_005193"
                     /pseudo
     CDS             93..866
                     /locus_tag="ACHY09_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            916..>1812
                     /locus_tag="ACHY09_005194"
     CDS             916..>1812
                     /locus_tag="ACHY09_005194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFS3888004.1"
                     /translation="MAGPADASQSVLKNNRSSLKGDFTESAELYLVLHDLSGHPINVS
                     EGLEFVQSGTNVSYVQISTIDYTQNLYGEYKATVTGGGEGIATLIPVLNGVHQAGLST
                     TIEFISAGTRPMNGTVSVNGANLPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSS
                     TASWVDVDTSGKVTFKNVGDRNAVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLS
                     QAENYCSNQVGNGYPLPRADLLSNGHMRREIGSLYGEWGDMGNYMKEADFYSMVYWSS
                     NSAGAGQQYIVSLETGTQNTYQT"
BASE COUNT          496 a          421 c          455 g          440 t
ORIGIN      
        1 attaattttc aatcatattg ctatcaggat ttacattttt tcgctgtgct agaaagggcg
       61 catttatgtt agctcgttca gggaaggtaa gcatggctac gaagaagaga agtggagaag
      121 aaataaatga ccaacaaatc ttatgcggga tgggtattaa actacgccgc ttaactgcgg
      181 gtatctgcct ggtaactcaa cttgtgttcc ctatggctgc ggcagcacaa ggtgtggtaa
      241 acgccgcaac ccaacaacca gttcctgcac aaatcgccat tgcaaatgcc aatacggtgc
      301 cctataccct tggagcgctg gaatcggccc aaagcgttgc cgaacgtttc ggtatttcgt
      361 tggctgagtt acgcaaactc aaccagtttc gtacgtttgc tcgaggtttt gataatgtcc
      421 gccagggtga tgaactggat gtcccggcac aagttagtga aaaaaaatta accccgccgc
      481 cgggtaatag cagtgacaac cttgagcaac agatagccag tacttcacag caaatcgggt
      541 ctctgctcgc cgaagatatg aacagcgagc aagcggccaa tatggcgcgt ggatgggcct
      601 cttctcaggc atctggcgca ttgacagact ggttaagccg cttcggtacc gcaagaatca
      661 cgctgggcgt ggatgaagat tttagcctga agaattccca gttcgatttt ctccatccat
      721 ggtatgaaac gcctgataat ctctttttca gccagcatac cctccaccgt actgacgaac
      781 gtacgcagat taacaacggc ttgggttggc gtcatttcac tcccacatgg atgtcgggca
      841 tcaacttctt tttcgaccac gatcttaagc gcagaaacga gctatggtgg ggcgcaaaca
      901 gtagatataa cgctggtggc aggcccggca gacgcctcgc agtccgtcct taaaaacaac
      961 cggtcatcac tgaaagggga ctttaccgaa agtgctgaac tatatcttgt tttacacgat
     1021 ctatcaggtc atccgatcaa tgtttctgaa gggctggaat ttgtgcaatc aggtaccaac
     1081 gtgtcctatg tgcaaatcag cacaattgat tacactcaaa atttatacgg tgagtataaa
     1141 gccactgtta caggcggcgg agagggtatc gcaacgctga tccccgtgtt gaatggtgtt
     1201 caccaggcgg gtctgagtac cacgatagaa ttcattagcg cgggaaccag accaatgaac
     1261 ggtacagtat cagtgaatgg tgcaaaccta cctgcagctt cattcccttc gcagggattt
     1321 accggggcgt attatcagtt gaataatgac aactttgcac caggaaaaac ggcggctgat
     1381 tatgcgtttt caagcactgc ctcctgggtt gatgttgata cttccggtaa ggtgacattc
     1441 aaaaatgttg gagatagaaa tgctgtaata atcacggcga ccccaagatc aggtggcgct
     1501 atttaccaaa cgcaagtacg ggtgaagggc tggtgggtga atcacggtaa taacctcatg
     1561 caattaagcc aggctgaaaa ctactgcagc aaccaggtcg gtaatggcta cccccttccc
     1621 cgcgcggacc ttttaagcaa tgggcacatg cggcgcgaaa tagggtcact gtatggtgag
     1681 tggggagata tgggaaatta catgaaggaa gctgattttt attcaatggt atattggtct
     1741 tctaattccg caggcgcagg ccaacaatac attgtatccc tggaaacagg gacgcaaaac
     1801 acttatcaaa cg
//
LOCUS       DAZUCC010000424         1793 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZUCC010000424 DAZUCC010000000
VERSION     DAZUCC010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.426"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..88)
                     /locus_tag="ACHY09_005195"
     CDS             complement(<1..88)
                     /locus_tag="ACHY09_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888005.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNS"
     gene            complement(72..1793)
                     /locus_tag="ACHY09_005196"
                     /pseudo
     CDS             complement(72..1793)
                     /locus_tag="ACHY09_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          350 a          570 c          419 g          454 t
ORIGIN      
        1 ttgaattgtt atcaaaccca aggtaaacaa caaataaaca aagggtaatt aaaaaaatga
       61 caagtttgcc tctaataatt tgaagcatct gaactccctt gctttgcttt tattggatta
      121 tctgctttta taccatctaa cccgaagagt tgcccagtgt tatatttttc aatacatgca
      181 tcgctacatt tggcagaaaa atcgccacaa gcaataccga cagtgttatt tttaagatcc
      241 atgattcttt ctttagctgg ctgaccttgt ctatcccctg ccgcctcatg atttttacct
      301 atcacgtcag ctattgttga tccaaatgtc ttagtcattc gacacatcaa gtaacaatgt
      361 ctcattgcat cggcggcccc attatgagct ccgccaacaa tagcatccat atcataaccg
      421 aactggtaag cactgttagt aatgctgttt gcagcaatca atccaggtac accagatacg
      481 gcataaatta gtccacctga tgtcatattc tcaatcgcat ctaaccccat tgggtctatc
      541 acttgtatgg gattcaacgg atactgatac atattccatc cacctctcaa cccaatcggg
      601 tccggggtga tataccgccc ctgcaacgga tcgtagtacc ggtgacggtt atagtacagc
      661 cccgactcct catcatgctg ctgccctggc agacggtacg gctggtgcag gtgatgcggg
      721 ttttcctcat tcagcaggtt tccccactca tcatactccc cgctccacgc cgtattgccg
      781 tcttcgctga taagcgccag cggcagtccc cggtggtcgc agtggtaaag atgaactttt
      841 cgcgccggtg tgtattccgg ctccacctgt ttttccagtt gctccaccgt cagcccgcac
      901 tgcgcaagcc acgcccggct ttcactgctc acgcggtctg tccggatttc ttcctccagc
      961 ctgtccagca gccgcaccag ttcagccggg aacaccacgc cgtgtccgtc ctcgctccct
     1021 tcctgctgga gcttctccgc caggctgcgg cgctgcgctt tctccagttc ggtcacgtct
     1081 gtctcgatgc ggatgagcgg cgtgaagctt cccggctggt ataccgtctg gatacgcgtg
     1141 gtgtcggcct gcaccgtcgt cagcctgtca ccgtcccagc cgtaccacgt ctcctccggt
     1201 ttacgcgaca gcgacatcca tcctgtcagg tcacgcccgc gacgccacac ccgtttaccc
     1261 gtccggcggc ccagcgggtc gtaaaggtag cggctctcga ccagtggctc gccatgctgg
     1321 caccgtctcc cggtgcaggc ggtcgcgcgt gtactccagc agcggcgtcc cgcccagctt
     1381 catgcccgcc agataaccgc tgccataggt cagccactcc accggcggca ggctgtccgg
     1441 cgtgacgcgg tttgccagcc cctgttcgtt gtatgcctgt ttcgtctcat gctgccacag
     1501 cagttccccc gtctccgggt tctccaccgt ctggcgctcc ccggtcagac ggcccttatc
     1561 gtcgtagcca tagtggacgg caacacggtg gccttcgctc aggtggctga tttctctcag
     1621 ccagccgtgg ccatcatact gccactgctc tgccggttcg ccgttcaccg tgcggtgagt
     1681 gatacggtcc gattcatcgt agtaccagag gatgacaagc ccctcatctt cactctgcgt
     1741 gagttttccg gtcaggtcat aatgataacg ttgcgtccgc ccgtcaaagc cgc
//
LOCUS       DAZUCC010000425         1786 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZUCC010000425 DAZUCC010000000
VERSION     DAZUCC010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.427"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..429
                     /locus_tag="ACHY09_005197"
     CDS             <1..429
                     /locus_tag="ACHY09_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3888006.1"
                     /translation="ENCMSGISSATPGENTVKEDSVSHKETLSGDVLFNFDSWRLTDK
                     ANDELERIATKFSQVDDVRIEGFTDPLGSKEYNRILSEKRAESVKEWFISRGFKAERI
                     QATGRGADAPVALCPGMVGDGLKECLRPNRRVEITGQIVR"
     gene            620..1294
                     /gene="tnpA"
                     /locus_tag="ACHY09_005198"
     CDS             620..1294
                     /gene="tnpA"
                     /locus_tag="ACHY09_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFS3888007.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSRE
                     FKVRLAKQALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPECAPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
     gene            1291..1638
                     /gene="tnpB"
                     /locus_tag="ACHY09_005199"
     CDS             1291..1638
                     /gene="tnpB"
                     /locus_tag="ACHY09_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS3888008.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFSKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
BASE COUNT          471 a          418 c          477 g          420 t
ORIGIN      
        1 gaaaattgta tgtcaggtat atcaagcgct actcctggtg agaacacagt aaaagaggat
       61 tctgtctctc ataaagaaac tctgtcgggg gacgtattat ttaactttga ctcgtggcgg
      121 ttgacggata aagctaatga tgaactggag cgaattgcca caaagttcag tcaggttgat
      181 gatgtaagaa ttgaggggtt tacggatcct cttggaagta aggagtataa tcgtatactg
      241 tcagaaaaac gtgcagagtc tgtcaaggaa tggtttataa gccgaggatt taaggccgaa
      301 aggatacaag caacaggccg gggggctgat gcaccagtag cattatgccc tggtatggtg
      361 ggggatggac tgaaggagtg tctgcgcccc aaccgtaggg ttgaaataac tggacagatt
      421 gttcgttaat atttttgtag ccccggaata tccggggctt ttttattaag aagggaggtt
      481 tgctttgctg tgtaagtttg gcttttgtgt ttgcagggta aacaaaacag attaactgta
      541 agcgccacat ctgcgacgtc ttgtgaaaat tgtcctgtct ggcaacaatc gcgcccatct
      601 atatagatgg acacgaacga tgaattccca gacaaaaaaa gatattccct gcttccgttc
      661 ttatttgcct gatgctctgc gtttaagatt tgaagataaa ctgaccatcc gggccatcgc
      721 tcagcgtcta ggtctcagtc attccacaat acatacgctt tttcagcgat ttattgcatc
      781 cggtatcgca tggccattgc ccgattcagt ttcattagct cagcttgacg ccatccttta
      841 tgccaacaga aagaaggaat taacagagcc tcaaatcagc gaaggcacat ggcgaaaaga
      901 acggcgagcc agctacagcc gtgaatttaa ggtccgtctg gctaagcagg cattacagcc
      961 cggtgctgtt gttgcccgga tcgccagaga gcacggtatc aatgataacc tgctgtttaa
     1021 atggaaaagc cagtacgagg acggcttact gagcgatgat gacatacagg aatgcatgcc
     1081 tgtcccggtg gctctgactg atacgccaga gccgaccaga ccagttacaa atcccttctg
     1141 gcgtaacaag cctgatgagt gccctgagtg tgctcccgga aacgtcccac ggtgcgagct
     1201 gcatcttaaa tcaggtgtgg taaaactgtt tgaccctctc actccggaaa tgttacgggc
     1261 gctaatccgc gaaatgaaag gaggtacccg atgataacgc tgccaaccgg taccagaatc
     1321 tggatcatcg ctggcatcac agatatgcgt tgtggcttca acggcctggc ttcgaaggtg
     1381 cagaacacgc tgaaagatga cccgttctcc gggcatatct tcgtcttccg gggccgcagt
     1441 ggcaaaatgg tgaaaatact gtgggccgat cgtgacgggt tatgcctgtt ctccaaacgc
     1501 ctggaacggg gccgcttcgt ctggccggta acccgggaag ggaaagtgca cctgacgcca
     1561 gctcagttat ccatgctact ggaggggatc gcgtggcaac atcccaaacg gacagaacgg
     1621 cctggcatcc ggatataacc cgtgataaaa caggggaatg aacaacacac tccccgacga
     1681 catcgagcaa ctgaaggccc tgctgatcgc acagcaggcg gttatcgtcc gtctgtctgg
     1741 tgaaataacc ggctatgccc gcgagatcag ctcgctcaga gcgctg
//
LOCUS       DAZUCC010000426         1786 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZUCC010000426 DAZUCC010000000
VERSION     DAZUCC010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.428"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..129
                     /locus_tag="ACHY09_005200"
     CDS             <1..129
                     /locus_tag="ACHY09_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS3888009.1"
                     /translation="EMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKENHSRYVPG"
     gene            93..1181
                     /gene="tssG"
                     /locus_tag="ACHY09_005201"
     CDS             93..1181
                     /gene="tssG"
                     /locus_tag="ACHY09_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFS3888010.1"
                     /translation="MERESQPVRTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIGNRSGLGAGNRIRLSQRAPLGKTTGEACSRLLMTLETADPNEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPPAEDGHYRFE"
     gene            1217..1741
                     /gene="tssJ"
                     /locus_tag="ACHY09_005202"
     CDS             1217..1741
                     /gene="tssJ"
                     /locus_tag="ACHY09_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFS3888011.1"
                     /translation="MVTVIRWWVPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDVAGYQMLLRNADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
BASE COUNT          376 a          503 c          531 g          376 t
ORIGIN      
        1 gagatgctgc accgtttctt tgcgctctat gctgatgttc accttttcaa ccagctcaca
       61 ctggtactgc aacccacagg gaaacgactg agatggaaag agaatcacag ccggtacgta
      121 ccgggctgac gcaggccctg gcccttaaca acgatatctg gcgggccagt ttttatcgtt
      181 tctgccagct gctggagcag gaaaatccgg acgggccaaa actgggcacg accagccatc
      241 cgggcgatga tccggtgcga ttcagaccct ggcccgggat gggattcccg gtcagcaccc
      301 tgaaagcggt ggagatcgac gaagaccgtc cgacattacc cccaacaatc cgcacgacat
      361 ttctcggaat gtatggggtg gactcacctc tgccgacgtc ctggctggat gatattacgc
      421 agcgccgtga aggacatgag gcactcacct catttctgga tattttcagc catcgcatca
      481 ccacgcagta ctaccggatc tggcgaaaat atgcgtaccc ggccactttt gaggaggggg
      541 ggcgcgatgc cacatcgcag tgtctgctgg ggctggtcgg gctcggtata cccggcaccg
      601 ctgaacaggt ggcaacgccg gtttcccgtt tcctggcctt actgggtgcc atgcgattac
      661 ccacccgcaa tgctgaaggc atccgggctc tggtgagtct gctggcaccg gatacccgcg
      721 cacttatcac cgaacccgat ccggttaagg tccatatcgg taaccgcagt ggactgggtg
      781 ccggaaaccg catccgtctt tcgcagcggg cgccgctggg taaaacgaca ggggaagcat
      841 gcagccggct cctgatgaca ctggaaacag cagatccgaa cgaagcggaa ggctggttgc
      901 ccggtggtgt cctccatacc gatttgctgg tcctgctgcg tgtttatctc ggctaccgca
      961 gtgacgtccg gctgcgactc actgtccccg tgcggttgtt gcctgagccc cgcctgggca
     1021 aaggcaggcc tgttcagctg ggccgtaccg gtttgctggg gctgaaagcg ggcaagctca
     1081 gcaatggccg ggagagtctg acggtcagtc tgggctgcta cgaagggttg aaatgtgctg
     1141 cgctaccgcc tgctgaagac ggacattatc gcttcgaata atcacctgtt ctctctctca
     1201 ataaaggagc gcctgaatgg taacagtaat ccgctggtgg gtgccgatgt ttattgcctg
     1261 tggcctgctc gcaggctgcg gtctgacgca gaccgtcacg gacggcacga tcagcatcac
     1321 gaaatccatc ttctacaaaa aaatcaaaac gctgcatctg gattttaccc cacgcacggc
     1381 cattaacact gacggggcac agacgccact ggcaaccatg gtgcgggttt accagctcag
     1441 ggatcgtaaa gccgttgatg tcgccggtta ccagatgctg ctgcgtaatg ccgatacggt
     1501 acttaaggac gacgtgctgg cctcaaaaga gctgctggtg atgcccggcg gcagcgtgac
     1561 gctcaatatg cccatggatg aagatgcgca gtttgtggct gtggtgggat tgtttaaccg
     1621 gccggatcag aaggacaatc gctggcgcct ggtactgacc cgtgatgacc tcaatccgga
     1681 taagccccgc accattgaac tgggtgatgg ctggctcagc cttgtgccgg taaaggagtg
     1741 acgtgatgga taaccggtca gcgttttttg acgggcagac ccgtta
//
LOCUS       DAZUCC010000427         1784 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZUCC010000427 DAZUCC010000000
VERSION     DAZUCC010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.429"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..129
                     /locus_tag="ACHY09_005203"
     CDS             <1..129
                     /locus_tag="ACHY09_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS3888012.1"
                     /translation="EMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKENHSRYVPG"
     gene            93..1181
                     /gene="tssG"
                     /locus_tag="ACHY09_005204"
     CDS             93..1181
                     /gene="tssG"
                     /locus_tag="ACHY09_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFS3888013.1"
                     /translation="MERESQPVRTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIDNRSGLGAGKRIRLSQRAPLGKTTGEACSRLLMTLETADPDEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPSAEDGHYRFE"
     gene            1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHY09_005205"
     CDS             1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHY09_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFS3888014.1"
                     /translation="MVTVIRWWAPMFIACGLLAGCGLTQTVTDGMISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDAADYQMLLRRADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
BASE COUNT          377 a          495 c          533 g          379 t
ORIGIN      
        1 gagatgctgc accgtttctt tgcgctctac gctgatgtcc accttttcaa ccagctcaca
       61 ctggtactgc aacccacagg gaaacgactg agatggaaag agaatcacag ccggtacgta
      121 ccgggctgac gcaggccctg gcccttaaca acgatatctg gcgggccagt ttttatcgtt
      181 tctgccagct gctggagcag gaaaatccgg acgggccaaa actgggcacg accagccatc
      241 cgggcgatga tccggtgcga ttcaggccct ggcccgggat gggattccct gtcagcaccc
      301 tgaaagcggt ggagatagac gaagaccgtc cgacattacc cccaacaatc cgcacgacat
      361 ttctcggaat gtatggggtg gactcacctc tgccgacgtc ctggctggat gatattacgc
      421 agcgtcgtga aggacatgag gcgctcacct catttctgga tattttcagc caccgcatca
      481 ccacgcagta ctaccggatc tggcgaaaat atgcgtatcc ggccactttt gaggaggggg
      541 gacgcgatgc cacatcgcag tgtctgctgg ggctggtcgg actcggtata cccggcaccg
      601 ctgaacaggt ggcaacgccg gtttcccgtt ttctggcctt actgggtgcc atgcgattac
      661 ccacccgcaa tgcggaaggc atccgggctc tggtgagtct gctggcaccg gatacccgcg
      721 cacttatcac tgaacccgat ccggttaagg tccatatcga taatcgcagt gggctgggtg
      781 ccggaaaacg catccgtctt tcgcagcggg cgccgctggg taaaacgaca ggggaagcat
      841 gcagccggct cctgatgaca ctggaaacag cagatccgga cgaggcggaa ggctggttgc
      901 ccggaggtgt tctccatacc gatttgctgg tcctgctgcg cgtttatctc ggctaccgca
      961 gtgacgtccg gctgcgcctt actgtccccg tgcggttgtt gcctgagccc cgcctgggca
     1021 aaggcaggcc tgttcagctg ggccgtaccg gtttactggg gctgaaagcg ggcaaactca
     1081 gcaatggccg ggagagtctg acggtcagtc tgggttgcta cgaagggctg aaatgtgctg
     1141 cgctaccgtc tgctgaagac gggcattatc gcttcgaata atcacctgtt ctctctcaat
     1201 aaaggaacgc ctgaatggta acagtaatcc gctggtgggc gccgatgttt attgcctgtg
     1261 gcctgctcgc aggctgcggt ctgacgcaga ccgtcacgga tggtatgatc agcatcacga
     1321 aatccatctt ctacaaaaaa atcaaaacgc tgcatctgga ttttaccccg cgtacggcca
     1381 ttaacactga tggggcacag acgccactgg caaccatggt gcgggtttat cagctcaggg
     1441 atcgtaaagc ggttgatgcc gccgattacc agatgctgct gcgccgggcc gatacggtac
     1501 ttaaggacga cgtgctggcc tcaaaagaac tgctggtgat gcccggcggc agcgtgacgc
     1561 tcaatatgcc catggatgaa gatgcgcagt ttgtggctgt ggtgggactg tttaaccggc
     1621 cggatcagaa ggacaatcgc tggcgcctgg tactgacccg tgatgacctc aatccggata
     1681 agccccgcac cattgaactg ggtgatggct ggctcagtct tgtgccggta aaggagtgac
     1741 gtgatggaga accggtcagc gttttttgac ggacagcccc gtta
//
LOCUS       DAZUCC010000428         1782 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZUCC010000428 DAZUCC010000000
VERSION     DAZUCC010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.430"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1782)
                     /locus_tag="ACHY09_005206"
     CDS             complement(<1..>1782)
                     /locus_tag="ACHY09_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888015.1"
                     /translation="SSYNLAIYSNTGKVLGLGSFLASRLFAKHPDRINYSTDVLNEGW
                     RDITVEELRLSSEYVDSRGFYRFPSPITGNPASGFGPQLKVVGLFDAQQNLQKIAISF
                     CGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAVSNGLSGEDVIVTGYSLGGGLTN
                     IMARLSESLADGFYANSDYIAWASPLVFDGDGRVLNIGFENDPVFRVLGDESGFWEAV
                     KAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPFSVLALPLGSWSAHFAGSKTD
                     AFERILGSQFYDYMSPDSTVIVDEQSGWTSWYSWVHDKGDDSRERPVFIIGNDGNNLL
                     AGGQGGDYIEGAGGNDKIEPGKGANRVDGGSGYDTLLLGDVMDDWNIYRVSDGTLFFH
                     SERGAGLTEATGIEGISFSGDWRSTLSPYQIGEEQITDHRYLLKWRNKNVDYGKHTEG
                     TTGDDSLYGDIIFGLDGNDILRSAGGEGGSLLHGGCGNDYLHGASGNDELYGAEGNDW
                     LYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKGEEDFLFLSKDIVTSTDEFI
                     SCLKQTGNDVEAGIAGGQIYFVNTALE"
BASE COUNT          528 a          489 c          292 g          473 t
ORIGIN      
        1 cctcaagcgc tgtattaacg aaatatatct gtcctcctgc aatacctgcc tcaacatcat
       61 ttccggtttg tttcagacat gaaatgaact catctgtcga tgtaacaatg tcctttgata
      121 aaaacagaaa atcttcttca cccttatgtt tattaaaatc catgataaat gaattccctt
      181 cacaagcagc atcaaagacg aagacatcat gaccagtacc gccatacaga taatcattac
      241 ctcccccacc atataaccag tcgttacctt ctgccccgta caattcatca ttaccagaag
      301 ccccatgaag ataatcattc ccacaccctc cgtgtaaaag acttccccct tctcctccgg
      361 cactacgtaa tatatcattt ccgtcaaggc caaaaattat atctccgtac agactatcat
      421 ctccggttgt accttcagta tgtttgccat aatcaacatt tttattacgc cacttcagaa
      481 gatacctgtg gtctgtaatc tgctcttctc ctatctgata aggacttagt gtactcctcc
      541 agtcaccaga aaaagagata ccttcaatac ctgttgcctc agtaagaccg gctccccgtt
      601 ctgagtggaa aaacagtgtc ccgtcactga ccctgtatat attccagtca tccattacat
      661 cgccaagaag aagcgtgtca tacccactgc caccatcaac cctgtttgct cccttacctg
      721 gttcaatctt atcattccct cctgccccct ctatatagtc accaccctgc ccacctgcca
      781 gcagattatt gccatcatta ccgatgatga atacaggtct ttcacgactg tcatccccct
      841 tgtcatgaac ccatgaatac caggatgtcc agccactctg ctcatccaca atgactgtac
      901 tgtccggtga catgtagtcg taaaactgag accctaaaat tctttcaaag gcgtcagttt
      961 ttgaacccgc aaaatgcgca ctccagcttc ccagcgggag agcaagcaca gaaaagggga
     1021 taatattcca gaggaatgaa ccataaaggt ctgtgtataa aacaatgtta tcaggagaag
     1081 agtcatattc acgatccggg gttgtcaagc ccggccccat tgctttaact gcctcccaga
     1141 acccggattc atctcccagc acacgaaata ccggatcgtt ttcaaaaccg atatttaata
     1201 ccctcccatc cccgtcaaat accagcgggg aagcccaggc tatatagtca gaattagcat
     1261 aaaaaccatc agccagagac tcactgagtc tggccataat gttagtcaac ccgcccccca
     1321 ggctatagcc tgtgacaata acatcctcac cacttaaacc attactgact gcaaagtctc
     1381 tgatactttg aagcaggttt tctgcttcag acagcgcttt tccattattg agctgtgtat
     1441 aatcaacaat atcgcagata tcatttgttc cacagaatga tattgcaatt ttctgtagat
     1501 tctgttgagc atcaaacaac ccaacaactt tcaactgagg accaaatcct gacgcagggt
     1561 ttcctgtaat cggactggga aagcgataaa aacctcttga atcgacatat tccgaagata
     1621 acctgagttc ttccactgta atatcacgcc agccttcatt aagaacatcg gtagaataat
     1681 taatcctatc cgggtgcttt gcaaatagcc tggatgcaag aaaacttcct aatccaagaa
     1741 cctttccagt gttggaatat atagcaagat tataagaaga ag
//
LOCUS       DAZUCC010000429         1780 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZUCC010000429 DAZUCC010000000
VERSION     DAZUCC010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.431"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(125..604)
                     /locus_tag="ACHY09_005207"
     CDS             complement(125..604)
                     /locus_tag="ACHY09_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888016.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMLLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
     gene            complement(716..>1780)
                     /locus_tag="ACHY09_005208"
     CDS             complement(716..>1780)
                     /locus_tag="ACHY09_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFS3888017.1"
                     /translation="MAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVSRV
                     LLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACMSR
                     NRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQRVTEDRMQPG
                     SETEPGVETQSSVLKMLSSDSELSEKQGQQILSSDSELSEKSDGYHRVRKPNHYVRNI
                     THSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELAQGVLESLSGMLGSHSLQNP
                     VGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAERL
                     SGIVAGLRASLRRCSAMRSE"
BASE COUNT          393 a          537 c          366 g          484 t
ORIGIN      
        1 cagcactgtt cactttttaa cctcttctgc cactggcagc actgttcact ttttgacctc
       61 tgctgccact ggcagcactg ctcatttttt aacctctgtt gccactggca acaccgctca
      121 ttttttaatc actgttgcca ctggcaacat ggttaatgat gactactacc ttaacgttca
      181 gagacaaaaa cagcattaat gtcgttatcc ccatcacgag caactgaatg tcggttatat
      241 ccagatgaga caatgaccag aaaatcacaa aaaatgaaaa gcattctgcc agcagactca
      301 ggaggagcat aaattgcagg ccccctccga ctgtactgcc tgtacgcgct ttataaagac
      361 tgtatgtgac ccacagccag ttacttaaca ccaggaaaaa ccaccctgtg gcacatacga
      421 tataacacat actgttatcg tactgttcgt gcattgtttt cagcgcagtg agcagggtct
      481 ctgtgcctgt caccagcccc cagataacca ccactccggc aatcacgcag gcacaaattt
      541 tagcgatcaa caggccactt tctttcatgg ctgcactcag actttcctgt ttgtatttct
      601 gcatgctatt gaccaccttg tttcacctga ccagttcccg attctgtttt ctttttaacc
      661 agtcgctggc ggcactggtt gatctttgtt cccgcgaaca tagcccgtat tgcaatcact
      721 cagatctcat cgcagaacat ctgcgcagcg aggcccgtag tccagccaca atgccactca
      781 gacgttctgc tgaaatcggt tccgtacgtt tccccgatga aactgattgc gcagtagctc
      841 gccctctttc tcctgccagt acggaggctg gcacaacaac acgttgtttc tgcttcggcg
      901 gataaaaatt tccctcccgg gctctcttca gcactgcaag caaccagccc acaggattct
      961 gcagcgagtg gctgcccagc atccctgaca gactttccag aaccccctgc gccagttctt
     1021 ccggcagcgc ctgcaactga ctggtcagca taatggcctc ctcctccctg atttgccctg
     1081 tcagcgcttc aggcaatacg tacgtgtttt taatcacact gtgtgtgata ttacgtacgt
     1141 aatggttcgg tttccgaact ctgtgataac catctgattt ttctgatagt tcggaatccg
     1201 aactcagtat ttgttgcccc tgtttttcac tgagttcgga atccgaactc agcattttca
     1261 ataccgaact ctgtgtttct acccccggtt cggtttccga acccggctgc attctgtcct
     1321 cagtcactcg ctgacgggcc gccatctgtc ccggtgtctg ggcactccct atccgggctt
     1381 ccagttgact gatatggctg tgataatgac gcattcccgg atcatttttt atttcgcaca
     1441 gaatatcccg ggccgtctga cttatggtcc ggttccggct catacaggca tctgccacca
     1501 tatccagata acgtggatcc agtatttcag catcccggca cgtcaccggt tcatcgtgct
     1561 gggcataaat attgccccgt acccgccctt tctcatcacg cacccgtttg cacagactca
     1621 gccagcccgt gatccgcagc atcagcagca cccggctgac agtctccctg gaggcttttc
     1681 ctttccccgg ggaagccagc tgcagttgca gttcgtcata tgtggggaaa actgcacctt
     1741 cattattctg tgcatacagc cgaatcatca tccacgccat
//
LOCUS       DAZUCC010000430         1743 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZUCC010000430 DAZUCC010000000
VERSION     DAZUCC010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.432"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..482
                     /locus_tag="ACHY09_005209"
     CDS             <1..482
                     /locus_tag="ACHY09_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3888018.1"
                     /translation="TAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMSPITL
                     RSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSRASSK
                     NEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPLKGN"
     gene            485..>1743
                     /locus_tag="ACHY09_005210"
     CDS             485..>1743
                     /locus_tag="ACHY09_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFS3888019.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMAVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGFILIPGK
                     GWRLSEAYDLNPVVRSDGLKLNITENDNALDLELAREVAEYFRLSLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRA"
BASE COUNT          474 a          324 c          462 g          483 t
ORIGIN      
        1 aaacagcacc gttactccct tatacgaatc agatgcttag tgaatttggt gagcggctga
       61 aacttgctcg tttacgacgc aggcttaccg caaagcaagt tgctgaacgt gctgggatgt
      121 cgccaataac attgcgctct ctggagtcag gaagtgcagg ggtcacgata ggggcatatt
      181 tgtctgtgat gcaggttttg ggcctggaaa cggatttaaa taagcttgca gctgaagacg
      241 acttagggcg tcagctccag gattcccgtt tgattaaaaa aagagggctt tctcgtgcat
      301 caagtaagaa tgaagaaggt tctcagacag cctcattgca gatgaggcac gagaaagaag
      361 gtcatctcac cacggattta acagcactgg cagaaggctc gacgccatct attacaggag
      421 aaacatcaac ctctcttgca gcactattaa aaccgataaa aaaaccgtta aaaggaaatt
      481 aattatggca ttaatcggag tgtatgcgga ttgggaaggc ctggatggtc cggagcggat
      541 tggttattta cacagtcgcc gcactcggac gcgtgagata tttgagtttg agtacgataa
      601 aaaggcgctt gcagatcctt cacttaattt cattcagtta gatcctgaaa taatgttgta
      661 tgagggagca cagtacccaa ttcctccaaa ggataaattt ggtgcattta gtgattcctg
      721 cccggatcgt tgggggcgta tgctaatgaa gcgtcgtttt gagagggata ttcgagacgg
      781 gttatgtgat aaagactccc acctttatga gtctgattat ctactaggtg ttcatgatct
      841 ttatcgggtt ggggcccttc gatacaaacg tgaagacgct ggagagtttc ttgataatcg
      901 cattgatgtt gcagccccac catttaccga aatagcgagt ctggaaaggg ctagccgggc
      961 aattgaagaa gatcctgata ataaagagct aatggggcaa aaatggttaa gaatgctaat
     1021 cgctccgggg ggatcattag ggggggcacg tccgaaagcc agtgttgttg atgaagctgg
     1081 gcatctctac atcgctaaat ttcccagtgt aaaagatgag tatgatgttg gtggatggga
     1141 aatggccgtt aatgcacttg ctgttggctg tggactgaat gttgcacctg ctcaggcgca
     1201 taaatttgcc agcaattacc attgttttat ggttcgtcgt tttgaccgca ctaatgcggg
     1261 aaggcgtctg cattttgctt cggctatgac actaaccagg catcaggatg gtgaagatgc
     1321 ctctacaggt gtgagttatc tggaattagc agatgttctt atcaggcatg gcgcgcagac
     1381 taatactgat cttaaggaac tctggtccag gattgttttt aatattcttg tttctaatac
     1441 cgatgatcat ttacgcaatc atggatttat cttgatacca ggaaaaggtt ggcggctttc
     1501 tgaggcatat gatttgaatc ctgtggtcag gtctgatggt ttaaaactca atattacgga
     1561 aaatgacaat gcacttgatt tggaattagc tagagaagtt gcagagtatt ttcgacttag
     1621 tttgacggaa gctgatgaca ttattgctaa ttttagaggg attgtcagtc agtggcgaat
     1681 cattgccgag cgtctgagat tacctggtcg tgagcaagag ttaatggcag aggcattcag
     1741 agc
//
LOCUS       DAZUCC010000431         1720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZUCC010000431 DAZUCC010000000
VERSION     DAZUCC010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.433"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..>1720
                     /locus_tag="ACHY09_005211"
     CDS             38..>1720
                     /locus_tag="ACHY09_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFS3888020.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYNKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSL"
BASE COUNT          553 a          374 c          344 g          449 t
ORIGIN      
        1 aataaatgtt cgtgatgcaa ctcagaagga ataccatatg gaacatcagt taaataaaaa
       61 actgcttaac tcatcaatat cacttatatt agcatctggt ctgatgatga gtactgcaaa
      121 tgcagcagat gatgttaagc tcaaagcaac aaaaaccaat gttgcctttg cggacttcac
      181 gccgacggaa tacagcacta aaggtaagcc aaatatcatt gttttaacaa tggatgattt
      241 agggtatgga caattgcctt ttgatgatag ttcatttgac cctaaaacga tggataaccg
      301 aaatgttgtt gatacataca aaataggtat tgataaagca attgaagctg caaaaaaatc
      361 cactccaaca ttagcggccc tgatggacga aggtgtaaaa ttcactaacg gctatgttgc
      421 acacggtgtc tctggccctt cacgtgccgc aatcctgacg gcgcgagctc ctgctcgctt
      481 cggtgtatat tcaaattctg atgcacagga tggtatccct ctggaagaaa cattcctgtc
      541 tgaattactt cagaatcatg gttattacac tgcagcaatc ggtaagtggc acttatcaaa
      601 aatcagcaac gtaggtgtgc ctgaagaaaa acaaacccgt gactaccacg ataacttcac
      661 caccttctcc gcagaagaat ggcaacctca gaatcgtggc ttcaactact tcatgggtta
      721 tcacgcatcc ggcgtttcac tttataactc cccgtcattg ttccgtaacc gtgaaaacat
      781 taaagctgaa gggtacatta gtgatcaatt aactgatgaa gcaattggtg taattgaccg
      841 tgcaagcaaa ctggaacagc catttatgat ttatctggca tacagtgcac cacacctgcc
      901 taacgatcag cctgcgccta aaaactatca ggagaaattc aacactggta gcaagactgc
      961 tgataactat tatgcgtcaa tttactccgt tgaccagggg gttaaaaaga tcctgaatac
     1021 acttaaggaa aaaggtcagt acgacaacac catcattctg ttcacctctg ataacggtgc
     1081 tgtaattgat ggtccactgc cactgaatgg tgcgcaggct ggttacaaag gccaaccgta
     1141 tccgggtggt atgcatacac ctatgttcat gtggtggaat ggcaagctga aaacgggtga
     1201 ttataacaaa ctggtttcat caatggactt ctatccgacc gctttagatg ccgcagatat
     1261 caagatccct gcttctctga aactagatgg tgtatcatta ttgccatatg tacagaaagg
     1321 agctgataag aaatctgcag ctggcgaacc tcacaaatat ctgtcctgga ttacctctta
     1381 ttctcactgg tgggacgaag agaacactgc attctggaac ggatatcata aattcgtgag
     1441 aaaagagtct gatgattatc cgcacaaccc acatacagaa gacttaagcc agtttgcata
     1501 cactgtaaga agcaacgatt attctctggt ttacacctat gcagagaata aactagctct
     1561 gtacgatatc aacgatctgc aacaaactaa agatctctct gcatcccatc ctgatgtagt
     1621 taagaatatg aaggctgtta tgattgactt catcacgtca agtcaaaaac cgatcagtga
     1681 tatcaaccaa gccaaatttg agaatataaa aaaatcttta
//
LOCUS       DAZUCC010000432         1709 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZUCC010000432 DAZUCC010000000
VERSION     DAZUCC010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.434"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1678
                     /locus_tag="ACHY09_005212"
     CDS             <1..1678
                     /locus_tag="ACHY09_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS3888021.1"
                     /translation="SALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAFSD
                     FTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEA
                     AQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPL
                     TETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTYSAEEWQPQNR
                     GFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFML
                     YLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEKLKKNGQYD
                     NTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLIS
                     AMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEENI
                     PFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQ
                     QKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          516 a          401 c          364 g          428 t
ORIGIN      
        1 gtctgcatta aagaaaagtg tcgtaagtac ctcgatatct ttgatactgg catctggtat
       61 ggctgcattt gctgctcatg cggcagatga tgtaaagctg aaagcaacca aaacaaacgt
      121 tgctttctca gactttacgc cgactgaata cagtaccaag ggaaagccaa atattatcgt
      181 actgactatg gatgatcttg gttatggaca acttcctttt gataagggat cttttgaccc
      241 aaaaacaatg gaaaatcgtg aagttgtcga tacctacaaa atagggatag ataaagccat
      301 tgaagctgcg caaaaatcaa cgccgacgct cctgtcatta atggatgaag gcgtacgttt
      361 tactaacggc tatgtggcgc acggtgtttc cggcccctcc cgcgccgcaa taatgaccgg
      421 tcgagcgccc gcccgctttg gtgtctattc caataccgat gctcaggatg gtattccgct
      481 aacagaaact ttcctgcctg aattattcca gaatcatggt tattacactg cagcagtagg
      541 taaatggcac ttgtcaaaaa tcagtaatgt gccggtaccg gaagataaac aaacgcgtga
      601 ctatcatgac aacttcacca cctattctgc ggaagaatgg caaccgcaaa accgtggctt
      661 tgattacttt atgggattcc acgctgcagg aacggcatat tacaactccc cttcactgtt
      721 caaaaaccgt gaacgcgtcc ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc
      781 aattggcgtt gttgatcgtg ccaaaacact tgaccagcct tttatgcttt acctggctta
      841 taatgctccg catctgccaa atgataatcc tgcaccggag caatatcaga agcaatttaa
      901 taccggtagt caaacagcag ataactacta cgcttccgtt tattctgttg atcagggtgt
      961 aaaacgcatt ctcgaaaaac tgaagaaaaa cggacagtat gacaatacta ttattctctt
     1021 tacctccgat aatggtgcgg ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg
     1081 ctataagagt cagacctatc ctggcggtac tcacacccca atgtttatgt ggtggaaagg
     1141 aaaacttcaa cccggtaatt atgacaagct gatttccgca atggatttct acccgacagc
     1201 tcttgatgca gccgatatca gcattccaaa agaccttaag ctggatggcg tttccttgct
     1261 gccctggttg caagataaga aacaaggcga gccacataaa aatctgacct ggataacctc
     1321 ttattctcac tggtttgatg aggaaaatat tccattctgg gataattacc acaaatttgt
     1381 ccgccatcag tcagacgatt acccgcataa ccccaacacc gaggacttaa gccaattctc
     1441 ttatacggtg agaaataacg attattcgct tgtctataca gtagaaaata atcagttagg
     1501 tctctacaaa ctgacggatc tacagcaaaa agataacctt gccgccgcca atccgcaggt
     1561 cgttaaagag atgcaaggcg tggtaagaga gtttatcgac aacagccagc caccgcttag
     1621 cgaggtaaat caggagaagt ttaacaatat caagaaagca ctaagcgaag cgaaataact
     1681 aaaccttcat gcggcggatt tttccgccg
//
LOCUS       DAZUCC010000433         1694 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZUCC010000433 DAZUCC010000000
VERSION     DAZUCC010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.435"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            75..1658
                     /gene="yegH"
                     /locus_tag="ACHY09_005213"
     CDS             75..1658
                     /gene="yegH"
                     /locus_tag="ACHY09_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFS3888022.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
BASE COUNT          384 a          416 c          462 g          432 t
ORIGIN      
        1 ttgcattttt gccccgattt atccatgatc gaattgtgac atttgtcata caacgaatag
       61 gttttgtact tactatggaa tggattgccg atccgtctat ctgggccggg ttaatcacgc
      121 tgattgtgat cgaactggtc ctcggcattg ataacctggt ctttattgcc atcctcgccg
      181 aaaaactacc gccgaagcag cgcgaccgcg cacgggttac cgggctgctg ctggcgatgt
      241 taatgcgcct gttactgctg gcgtcaatct cctggctggt caccctgact caaccgctgt
      301 tcagcttccg ctcgtttacc tttagcgccc gcgacttaat catgctgttt ggtggtttct
      361 tcctgttgtt caaagccacg atggagctga acgaacggct ggaagggaaa gacagcaata
      421 accccaccca acgcaaaggt gcgaagttct ggggggtggt gacacaaatt gtggtactgg
      481 acgccatctt ctcactcgac tcggtgatta ccgccgtcgg gatggtcgac catttactgg
      541 tcatgatggc cgccgtggtt atcgctatca gcctgatgtt gatggccagc aagccgttaa
      601 cgcaatttgt taacagtcac ccgaccatcg ttattctctg cctaagtttc ctgttgatga
      661 ttggctttag cctggtggca gaaggtttcg gcttcgtcat tccgaaaggc tacctgtacg
      721 ctgccattgg tttctcggtg atgatcgagg cgctcaatca gttggctatc tttaaccggc
      781 gacgttttct ttccgctaac cagacactgc gccagcggac taccgaagcg gtaatgcgcc
      841 tgcttagcgg gcaaaaagaa gatgcggaac tggacgccga aaccgcgtct atgctggtgg
      901 atcatggtaa ccagcagatc tttaatccgc aggaacggcg gatgattgag cgggtactta
      961 atcttaacca gcgtaccgtc agcagcatta tgacgtcgcg ccacgatatt gagcatatcg
     1021 atctcaacgc gccggaagaa gaaatccgcc agttactgga gcgaaatcag catacgcggc
     1081 ttgtcgttac tgatggcgat gacgcagaag atttgctcgg tgttgttcac gttatcgacc
     1141 tgttacaaca gtctctgcgc ggcgaaccgc tcaacctgcg ggtgttgatt cgccagccgc
     1201 tggtgttccc ggaaaccttg ccgttgttac ctgccctgga gcagttccgt aatgcccgca
     1261 cacactttgc ttttgtggtg gatgagtttg gctcggtgga agggattgtg acattaagtg
     1321 acgtcactga aaccattgcc ggtaacttac cgaacgaagt ggaagagatc gacgcccgcc
     1381 atgatattca gaagaatgcg gacggttcct ggacggcgaa tggtcatatg ccgctggaag
     1441 atctggtgca atatgtgccg ctaccgctgg atgagaaacg cgaatatcac accattgccg
     1501 ggctgttgat ggaatatttg caacgtattc caaaacctgg cgaagaagtt caggtggggg
     1561 attatttgct taaaacgttg caggtggaaa gccatcgcgt gcagaaggtg cagattattc
     1621 cgctgcataa ggatggcgag atggagtacg aggtgtgata aaacatcaaa tgcgcaggtt
     1681 caaactgcgc attt
//
LOCUS       DAZUCC010000434         1692 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZUCC010000434 DAZUCC010000000
VERSION     DAZUCC010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.436"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(51..1589)
                     /gene="cadC"
                     /locus_tag="ACHY09_005214"
     CDS             complement(51..1589)
                     /gene="cadC"
                     /locus_tag="ACHY09_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFS3888023.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT          493 a          345 c          357 g          497 t
ORIGIN      
        1 caaaaactca acaacaaata ttcccgagca tgaatcaacc cgggagttac ttattctgaa
       61 gcgagaaatt tgtcgagata aggtacaaca taaggaacag aagtctggaa tataccattt
      121 tcaatccagt aaagggtgtt tgcccctggg cgtaaattaa aggcggtgag atatgcatca
      181 gctgcttccc ggttcatccc cttcatttca taaaccttgc caagcaacac ataatttagc
      241 caggacattt caagatcaat gccagtattt atcgcctgat aagactcatc tgttttacct
      301 tttaccaggg cactgaccgc ttttatttga tatataatgg acaggttgtt caattccggc
      361 agtgtaacaa tgttatctat ttctgtgtta agtgctgcta attgtttttc gtctaaagga
      421 tgttgagaat ggcgcacgat atcaactaat gctttttctg ctctcgcgta ggtaaattct
      481 ggggatgatt gaacaatctc acctaataat tcactggcac gatccaatga tttatcatca
      541 ccatgcagta aataatcatg tgcctgataa aaattagtta ataacgcacc acgatgcggc
      601 aaaatttgct ggagcatctc ctgcattcgt tgtggccacg gttggtttaa cgcttttgat
      661 aaactctcca gtaaatcatt ttgaatcgcc agctgattac cgttagtgat gacataacgt
      721 ttatccagca tggttgaacc atctgcattg tctaccaatt ttaccgacat aaagcattgt
      781 tgagcacggt attggcgctg attaacaaac gcaatagata atgttttacc ggaactgctc
      841 ggttcatcaa tgttgtagtt gattttgtca tgcaccataa aggtggagaa ggtgttaagt
      901 gatgtcgcca ccaaatcacc cacgcctatc gcgtaagaga gctgatacgg ggaactccag
      961 ctgttacaac ttttatttac catattaatg tcaatatcgc gtggattgag caaaatacgc
     1021 gatttgctca tcggaagacg tgtatcaaga cttgaaaacg ctaccagtgc tacacagata
     1081 cctaacgaca acaggaaaaa aaaccatacc caaaaggtag tgaatcgttt gcttttaact
     1141 ggggattgtt caggtggcgt tgcggtgttt tgaatgttaa gactgtggga gggagaatct
     1201 gtggcaggaa ccgcctctgg tataggggga ggcgaagata gcattatttc ctctccctct
     1261 tcttcgctgt accagataac cggcaccatt aatttatagc cgcgctttgg tacagtagcg
     1321 atatagacag gactatcttc atcattatct tttaatgact tacgtagttc tgagatactc
     1381 tgcgtcacaa cgtgattggt gacaatactt ctcttccaga cattatcgat aagttcatcc
     1441 ctgctaagta cttcgccact gtgttgagca aagaaaacca gaaggtcgat taatctcggc
     1501 tcaagggtaa gttgacgccc attgcggcta atttggttta tggacggagt aacaagccat
     1561 tcgccaacgc gaactacagg ttgttgcata atagaaactc attcgaaaag ggaatggtgc
     1621 aatgataatt ggcataacct atttttacca tctatagatg ggtttattta catattattg
     1681 gtgaatgcaa ga
//
LOCUS       DAZUCC010000435         1685 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZUCC010000435 DAZUCC010000000
VERSION     DAZUCC010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.437"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1685
                     /gene="ehxA"
                     /locus_tag="ACHY09_005215"
     CDS             <1..>1685
                     /gene="ehxA"
                     /locus_tag="ACHY09_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFS3888024.1"
                     /translation="FNNATSTTKSAFNTASSSVRSAGKKLILLIPDNYEAQGVGINEL
                     VKAADELGIEIHRTERDDTAIANQFFGTAEKVVGLTERGVAIFAPQLDKLLQKYQKVG
                     SKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGEDISQNDIAKS
                     SIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNLPDLGPLGDG
                     LDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQYILAQRMAQG
                     LSTTAASAGLITSAVMLAISPLSFLAIADKFERAKQLESYSERFKKFNYEGDALLAAF
                     HKESGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTISGILEASKQ
                     AMFEHVADKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFSRQHAVERAV
                     AITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQQVFDPQKGT
                     IDLSTGNVSSVLTFVTPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWSVKGI"
BASE COUNT          528 a          305 c          395 g          457 t
ORIGIN      
        1 ttttcaataa tgcgacatcg actacaaaat cagcatttaa tacagcatca tcaagcgtac
       61 gttccgctgg aaaaaaactc atattattaa tacctgataa ttatgaagct cagggcgtgg
      121 gtattaatga gttggtcaaa gctgctgatg agcttggaat agaaatacac cgtactgaac
      181 gagatgatac agcgattgca aaccagtttt ttggtacagc agaaaaagtt gtaggattaa
      241 ctgaacgtgg tgtagcaata ttcgcaccac aacttgacaa acttctgcag aagtatcaga
      301 aagttgggag taaaatagga ggaaccgctg aaaatgtagg taataatctg ggaaaagccg
      361 gaacagttct ctcagcacta cagaatttta cggggattgc tttatcaggc atggctcttg
      421 atgaattgct gagaaaacaa cgggaaggag aggatataag tcagaatgat attgccaaaa
      481 gtagtattga acttattaat cagcttgtag atacagtatc aagtataaac agtaccgttg
      541 attcattttc tgagcagctt aaccagcttg gctcattttt atccagtaaa cctcgcttaa
      601 gttctgttgg tggaaaatta caaaatttac cagacctggg ccccctgggg gatgggctgg
      661 atgttgtctc cggaattctt tctgctgtat cagcaagttt tattctggga aacagtgacg
      721 cacatacagg aacaaaagct gcagcgggta tcgaactgac aactcaggtt cttggaaatg
      781 ttggtaaagc tgtttcgcaa tatattctgg ctcagagaat ggcacaggga ttatcgacaa
      841 cagctgcaag tgcgggtctg atcacatcgg ctgttatgct ggctatcagt cctctttctt
      901 tcctggctat tgcagataaa tttgagcgag ctaagcagct tgaatcatat tctgaacgat
      961 ttaaaaaatt caattatgaa ggggatgctt tactcgcagc ctttcataaa gaaagcggag
     1021 ctatagatgc agccctgaca acaataaata ctgtcctgag ttctgtatct gcgggagtta
     1081 gtgcagcctc cagtgcatcc ctcatagggg ccccgataag catgctggtg agtgcattaa
     1141 ccggtacgat atctggcatt ctggaagcat caaaacaggc tatgtttgag cacgttgcag
     1201 ataaattcgc tgctcggatc aatgaatggg aaaaggagca tggcaaaaat tattttgaga
     1261 atggctatga cgcaagacat gctgcgtttt tagaagactc tctgtctttg cttgctgatt
     1321 tttctcgtca gcatgcagta gaaagagctg tcgcaataac ccagcaacat tgggatgaga
     1381 agatcggtga acttgcaggt ataacccgta atgctgatcg cagtcagagt ggtaaggcat
     1441 atattaatta tctggagaat ggagggcttt tagaggctca accgaaggag tttacacaac
     1501 aagtttttga tcctcaaaaa gggactatag acctttcaac aggtaatgta tcaagtgttt
     1561 tgacatttgt aacaccaaca tttaccccag gagaagaagt cagagaaaga aaacagagtg
     1621 gtaaatatga atatatgaca tctcttattg taaatggtaa ggatacatgg tctgtaaaag
     1681 gcata
//
LOCUS       DAZUCC010000436         1656 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZUCC010000436 DAZUCC010000000
VERSION     DAZUCC010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.438"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1288
                     /gene="tssC"
                     /locus_tag="ACHY09_005216"
     CDS             <1..1288
                     /gene="tssC"
                     /locus_tag="ACHY09_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFS3888025.1"
                     /translation="LIDHHIAELDDQISRQLDAVLHHPEFQKVESLWRGVKSLVDKTD
                     FRRNVKIELLDLSKDDLRQDFEDAPEIIQSGLYLQTYVAEYDTPGGEPIAALVSAWEF
                     DASAQDVALLKNISRVAASAHMPFIGSVGPAFFRKETMEEVAAIKDIGNHFERAEYIK
                     WNAFRETDDARYIGLVMPRVLGRLPYGPDTVPVRSFNYVEEVKGPDHHKYLWTNASFA
                     FAANMVRSFVTNGWCVQIRGPQAGGAVQDLPIHLYDLGTGNQVKIPSEVMIPETREFE
                     FANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRINARLPYIFLLSRVAH
                     YLKMIQRENIGTTKDRRLLELELNTWLRGLVTEMTDPGDELQASHPLRDGKVVVEDIE
                     DNPGFFRVKLYAIPHFQVEGMDVSLSLVSQMPKAKA"
     gene            1304..>1656
                     /gene="tssK"
                     /locus_tag="ACHY09_005217"
     CDS             1304..>1656
                     /gene="tssK"
                     /locus_tag="ACHY09_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3888026.1"
                     /translation="MKIFRPLWSEGVLLSPQQFQQQAEWESFRSAGISALASPFPWGV
                     EQVEFNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADRDAVTVV
                     IALPHMQPGVANVEQ"
BASE COUNT          350 a          400 c          483 g          423 t
ORIGIN      
        1 gctgattgac catcatattg cagagcttga tgaccagatc agtcgtcagc tggatgccgt
       61 gcttcatcat ccggaatttc agaaagtgga atccctgtgg cggggcgtga agtctctggt
      121 ggataaaacg gacttccgcc gtaatgtgaa aattgagctg ctggatctgt ccaaagacga
      181 tctgcgccag gattttgagg atgcgccgga aattattcag agcggccttt atctgcagac
      241 ctatgtggct gaatatgaca cgccgggtgg tgaacccatt gctgccctgg tttcggcctg
      301 ggaatttgat gcatctgcgc aggatgtggc gttgctgaaa aatatttccc gggtggcagc
      361 atccgcacat atgccgttta ttggttctgt tggtccggct ttcttccgga aagaaactat
      421 ggaagaggtg gctgctatta aggatattgg caatcacttt gagcgtgccg agtacatcaa
      481 gtggaacgct ttccgagaga cggatgatgc ccgctatatt ggtctggtga tgccccgtgt
      541 gctgggacgc ctgccgtatg gccctgatac tgtacctgtc cgcagcttta actatgttga
      601 agaggtgaaa gggccggatc atcacaagta tctctggacg aacgcgtctt ttgcttttgc
      661 ggccaatatg gtgcgcagtt ttgtcaccaa cggctggtgt gttcagatcc gcggtcctca
      721 ggccggtggt gcggtgcagg atttgccgat ccatctgtat gatctgggaa ccggtaatca
      781 ggtcaaaatt ccgtcagagg tgatgatccc ggaaacgcgg gagtttgaat tcgctaatct
      841 gggttttatt ccgctctctt actacaagaa ccgggattat gcctgtttct tctccgctaa
      901 ctccacccag aaaccggcac tgtacgatac gccggatgca acggcgaaca gccgtattaa
      961 tgcccgtctt ccctatattt tcctgctgtc ccgcgttgcg cattacctga agatgattca
     1021 gcgggaaaat atcggtacca ccaaagatcg gcgtctgctg gagctggagc tgaatacctg
     1081 gctccggggc ctggtgacgg agatgaccga tccgggcgat gagcttcagg catcgcatcc
     1141 gctgcgtgac ggaaaagtgg tggtggaaga tattgaagat aacccggggt tcttccgtgt
     1201 gaaattgtat gccattccgc atttccaggt ggaaggaatg gacgtcagcc tgtccctggt
     1261 ttcgcagatg ccgaaggcaa aagcgtaagg caggtgtcgg gaaatgaaga ttttccgtcc
     1321 gctgtggagc gaaggtgttt tgctctcccc tcagcagttt cagcagcagg ctgaatggga
     1381 gtcttttcgc agtgccggta tctctgcgct ggcatccccg tttccctggg gagtggaaca
     1441 ggttgagttt aatgacagtc ttctgtcgtc cgggctgatc cagattacgc agttacgcct
     1501 ctggctggaa gacggaacac ttattgatgc tcaaaggagc gatctgcctc cggcgccacg
     1561 tgagttagat gccggtcagc tggctgaccg cgatgcggta actgtcgtta ttgcacttcc
     1621 ccacatgcag ccaggcgttg ccaacgttga gcagga
//
LOCUS       DAZUCC010000437         1649 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZUCC010000437 DAZUCC010000000
VERSION     DAZUCC010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.439"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(92..1498)
                     /gene="gndA"
                     /locus_tag="ACHY09_005218"
     CDS             complement(92..1498)
                     /gene="gndA"
                     /locus_tag="ACHY09_005218"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS3888027.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIV
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          441 a          399 c          384 g          425 t
ORIGIN      
        1 ttaagcagtg aaatttagtc tataagataa ttggtaaaaa aacccggcat aatatacgcc
       61 gggcctcaat tttattgttg attaaatcag attaatccag ccattcggta tggaacacac
      121 cttctttatc aatgcgctta taagtatgcg caccgaaata gtcacgctgt gcctggatca
      181 ggttcgcagg cagaacagcg gcacggtagc tgtcataata ggcaaccgca gcggcgaagg
      241 tcggaaccgg gataccgttc tgtactgcat aagcaacgac atcgcgcagc gcctgctggt
      301 agtcatcggc aatttgcttg aagtaaggag ccagcagcag gttagcgatc tgcggatttt
      361 cggcataagc atcggtgatt ttctgcagga actgcgcacg gataatgcag ccagcacgga
      421 aaatcttcgc gatttcgcca tagttcagat cccagttgta ctcttcagac gctgcgcgta
      481 gctgagagaa gccctgagcg taagaaacga ttttgcccag atacagcgca cgacgaactt
      541 tttcgatgaa ctcagccttg tcgcctgctg actgagcttg cggaccagag agaactttag
      601 atgcggcaac gcgctgatct ttcagagaag agatataacg tgcaaacaca gactcggtaa
      661 tcagcgacag cggttcgccg agatccagcg cactctggct ggtccattta ccggtacctt
      721 tgttagccgc ttcatccaga atcacatcaa ccaggtagtt accgtcttca tcttttttgg
      781 tgaagatatc tttggtgatg tcgatcaggt agctgcttag ttcaccgtta ttccactcgg
      841 taaacgtctg cgccagttct tcgttggtga gattcaggcc acctttaagc agagaatagg
      901 cttcagcaat cagctgcata tcgccgtatt caataccgtt gtgaaccatc ttcacatagt
      961 gacctgcacc atcggcacca atataggtaa cgcatggctc accgtcttca gccactgcgg
     1021 cgattttggt caggatcggt gcaaccagtt cataggcttc tttctgccca ccaggcataa
     1081 tggaaggacc tttcagcgca ccttcttcac caccggaaac accggtaccg ataaaattaa
     1141 agccttcggc agaaagctca cggttacgac gaatggtgtc ctggaagaag gtattaccac
     1201 catcaatgat gatgtcacct ttatcgaggt atggcttgag ggaatcaata gcagcatccg
     1261 tgcctgcacc tgctttcacc attaacagga tgcgacgagg cgtttccaga gattcaacaa
     1321 actctttcac cgtatagtaa ggaaccagtt tcttgcctgg attttcgaca atcacttctt
     1381 ccgtcttttc acgggaacgg ttgaaaatag agacggtata accacgactt tcgatgttga
     1441 gcgcgaggtt gcgccccatc actgccatac cgacgacgcc gatctgttgc tttgacattg
     1501 tttactcctg tcagggtttt accgcatgtg cggtatgaga ataacttaaa aagtttactt
     1561 gaaataaagt attatatata tgatattgac ggtaaaaaca aagttgtcga cagactgaat
     1621 caaatggaca tacaattgag tcctattca
//
LOCUS       DAZUCC010000438         1648 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZUCC010000438 DAZUCC010000000
VERSION     DAZUCC010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.440"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            381..>1648
                     /locus_tag="ACHY09_005219"
     CDS             381..>1648
                     /locus_tag="ACHY09_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888028.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDAHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTP"
BASE COUNT          411 a          371 c          440 g          426 t
ORIGIN      
        1 ttcagatttc cggaagtgag agtttttggt gtttccatac cagccacagt tgatgtgtgt
       61 tgtggcacct ggcagtacaa ttaagggaac aggggtaata aaaaacgggg gcatgaccaa
      121 cccccggtac aaggaaggag taactacaat ggaaaaatca atgaagacat tcagtgctat
      181 gttatatggc gaataaagac tgccacaggg aagtttgcag aaacagtgat gccgatcgtg
      241 tttctgacgc tcttctctga taagagtaat ttttcagatt tctggaaatc tgtattctgc
      301 acaggcgcaa ttgtatgccg cctttaaaac ttcaattcag tgactcacct gccattcaaa
      361 ttttcggata ccagacaacc atgccttata tcgatataac aactatgcgc gggatgatgc
      421 caggcgttat tgcatctatg ctgccagatc attctgctgt actggcagaa aactgtcatt
      481 ttcgctatgg agtgatcacg cctgaacacc agatgtcaga ggctgagaaa acatttgcga
      541 ttaagccgaa aaccattttt cattaccgtg acgatttctg gtttgcatgg acggatgtgg
      601 tggatgtgat ccgcagtccg gtcgctcagg acgcccacgg gcgtatttac tacactgacg
      661 ggcgttttcc taaagtgacg gatgcgacca ttgccacaaa aggggacggg aatcacccga
      721 catcatcgta tcgtctgggg attcccgcgc cgacaacagc acctgtctgt actgttcagc
      781 agggcggtga tgtttctgac gataacccga atgatgacga aacccggttt tatacggaaa
      841 cctttgtctc agattatggt gaagaaggtc cgccaggtcc ggcgtctctg gaggtaacac
      901 tccgtactcc ggggactgcg gtacagctga cgctgtctcc ggtgccattg cagaatgcca
      961 gtattaaacg ccgccggatt tatcgctctg catcaggtgg aggagaagcg gattttttac
     1021 ttgtggctga actggatgca tccgtgctca gttacacgga caaaataccg gggaaaaacc
     1081 ttggaccttc tctggctaca tgggattacc tgccgccgcc agagaatatg acaggccttt
     1141 gcctgatggc taatggtatt gccgccgggt ttgccggtaa tgaagtgatg ttttcggaag
     1201 cgtatctgcc gtatgcatgg ccggaagtga atcgtcacac gacggcagaa gatattgtgg
     1261 ctatctgtcc gctgggaacg tcactggtgg tggcgacaaa gggggagcct tatctgttca
     1321 gtggtgtatc gccttccaca atttctggct ccagaattcc ttccatgcag gcatgcctga
     1381 gccgaagaag tatggtggcg atggagggat tcgtactcta tgccgggaca aacggtctgg
     1441 tatctgttga tgtaaacggt aatacagcac tggcaacgga aaagattatt tcacctgaac
     1501 agtggcagag tcagtttaac ccggcgtcca ttgtggctta ttcctggcgt ggtgaataca
     1561 ttgcctgtta cacgaaaccg gatggtaagc aggatgtgtt tgtattcagt ccggtgaaca
     1621 tggatatccg ttatctcagt acaccgtt
//
LOCUS       DAZUCC010000439         1635 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZUCC010000439 DAZUCC010000000
VERSION     DAZUCC010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.441"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..1528)
                     /locus_tag="ACHY09_005220"
                     /pseudo
     CDS             complement(1..1528)
                     /locus_tag="ACHY09_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
BASE COUNT          470 a          384 c          316 g          465 t
ORIGIN      
        1 aagatatcca aatgcatctc tggaaaactt aagcataaaa tttttcatat cagaaattgc
       61 ttcttgtaaa aaaaggggat catcgccatg attatccagc aggatggcaa ttttttcgca
      121 catattttct ttcctcagct cttccctggg ttgcagcctt ttcatggtat gatgttcaat
      181 ttctttcagg agtaatgagg catttatctg acgtctggta ctgccttgcg tcagccagtg
      241 gatggcttgg gaaaaacact gttgtgcaac ggtgaaataa acatcattct tgatgctact
      301 attcagtgcc tgcttttgat tatgcctatg atggattagc gtcacaacca acttttgcaa
      361 tggtgaacca tcctgattga caatatcttg ggcatgtgat ttctcgcagt attgattgag
      421 taatgcaaca aaactgttaa ccagttcttt caatatatca ggtatctctt tttgtgttat
      481 aaattctcca ggaataccca acagttctag ataacagtaa taacatgctt catggaaagt
      541 tttctgaaat atctcctcaa ccttcaaacg ggctgcaaga gtgaagtcag ggtcaaacat
      601 atattttccg atatacaagc agagatcaga cagcccttgc ataagtacca tcggttcttt
      661 tgccagttca gctaatctta gtttataccc aaaatcagct ggggaatctc ccggaaacag
      721 acaactgagt ccgttaaaat ccattaatct gccatccatt gcaatattag aaggagaagt
      781 tgtcaatctc aggaaacgtg ttcgacaaaa tgccatttgc caggcctcac gacgtgccag
      841 ttcacataac ccctcaatgc aataaatccg gggatcagtt cgtgcttcag catcaatttc
      901 ttccggtggt acaggtagat atcctggcag cttgtggatc acagatctaa cccgacaggc
      961 atcgtgaatt agcagactgg aatactccgg ttttacttgg aagtaaggtg cccgttcaaa
     1021 atgcgccgga cgaacaacag gctcacgtac caacagggct cttcgtgatt tcctaccgga
     1081 gcgctcaaat gccttttcag tatagagatc tgtaagtaaa accgcccgaa cccgcacagc
     1141 actataaggt aatatttgag ccagtacttc tccccacaaa gcctcataat attgcatgaa
     1201 cagcgccgag tgcaccatta gaatgacgtt cgtcagtacc ttcgccaacc aacggattac
     1261 ttcctattcc tttaacctgg tagttaccat caaacccaca acgtgcacca cctccattac
     1321 gctgaatacc actgccacca tagcgctctg catggaacac tttggcagaa gctgtactgc
     1381 caggatcgat aatataactg caggcatttt gcagctcgtc tggatttctg acatcctctg
     1441 cacaccagat taatgaggcc ttgatctgcc tggcagtgaa aggcaccagg ttttcattca
     1501 gttcagaaag tgaacactga tgactcatct ggcagtattc tccgtttcaa ttttcataaa
     1561 agagtgttca taaaagagca agtaagtgtc ctgacagctt cttccttatt acagtaaaac
     1621 tctattaatt acaca
//
LOCUS       DAZUCC010000440         1628 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZUCC010000440 DAZUCC010000000
VERSION     DAZUCC010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.442"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..505)
                     /locus_tag="ACHY09_005221"
     CDS             complement(29..505)
                     /locus_tag="ACHY09_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888029.1"
                     /translation="MHKSETEKIDFILDEHERIFRKQGSLCMALGKVFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDA
                     RQELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQHEQDTIRKFTEENKSE"
     gene            complement(498..719)
                     /gene="traR"
                     /locus_tag="ACHY09_005222"
     CDS             complement(498..719)
                     /gene="traR"
                     /locus_tag="ACHY09_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFS3888030.1"
                     /translation="MSDEVDEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQEKQRKHYA"
     gene            complement(856..1371)
                     /gene="traV"
                     /locus_tag="ACHY09_005223"
     CDS             complement(856..1371)
                     /gene="traV"
                     /locus_tag="ACHY09_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFS3888031.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLADGNFRTMPVQTVTATTPSGSRPAVTKLPEQKLLAPR
                     PLFTAAREVKTIVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(1368..1619)
                     /gene="trbG"
                     /locus_tag="ACHY09_005224"
     CDS             complement(1368..1619)
                     /gene="trbG"
                     /locus_tag="ACHY09_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFS3888032.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
BASE COUNT          415 a          428 c          343 g          442 t
ORIGIN      
        1 ttatgaggtt ataaaacaca gcagattttc attcggattt attctcttca gtaaacttcc
       61 ggatggtatc ctgttcatgc tgacgggtgg cagactcctg cattgcatcc actttctcct
      121 gccagagacg ccggatatcc ttttcatccc gaccagtcag ttttttcccg gacagcagac
      181 ggttaagcag ttcctgtctc gcatccggta cgtcttcagt cagacgcata agccggagta
      241 attcctgacg actgagtacc atgcgtgatt cctccggtac gctgtaataa ttaaaaatac
      301 gactgaatac cccacaacag caggcaacgg acaggcacag ggatttcaga tacaggccag
      361 aaacacagaa tgtggcaaca ccacaaagaa tagctataac taaaaaaacc ttacccagtg
      421 ccatacacaa agaaccctgt tttctgaata tccgctcatg ttcatccaga ataaaatcta
      481 ttttttcagt ttctgattta tgcatagtgt tttctctgtt tttcctgata cgcctgacat
      541 tcaacgcaca acgtcacacc gggaaatatt ttcctcctgg cttccggaat aggatttccg
      601 catgcctcac agagataaac aggaatacca tgagcattta ttttctggcg tatccggttt
      661 attcctgtca tggtcagttg ttctgtcact gaatatgctt catcgacttc atcactcacg
      721 gaatattcct cccgacatgt tctgacacgc taccgcccat cccttttcac gcacagggca
      781 ctcgttttac aggcagattt tgaatacaga aagcccgccg gcattgccgg ctgatataca
      841 caacttcagg gccagttaat taatgcgtgg ttttccccac gcagacggtt taataacaaa
      901 aaacacgctg gatggctgat gataaacatc ctgattatca atataaggcg ctatccataa
      961 tgctgccgtc tgctcacccg ttcttagcgg acggggaggt gttaccggcg caactgaact
     1021 gaccggaaca atcgttttca cttcccgggc agcggtaaac aacggacgtg gtgccagcag
     1081 tttctgctcc ggaagtttcg tcaccgcagg tctgctgccg gacggagtgg tcgcagtgac
     1141 ggtctgcacc ggcattgtcc ggaagttccc gtcagccagg cgcggcagtg atgccgcaac
     1201 cggctttgct tctgaggact gctccagttt tttggccttc tcattggcct gctccatcgt
     1261 catacaggta tcggatgtgg tggcatcaca ctcaaaatcg gagttcgttc cggcacagcc
     1321 ggaaagtaat aacgtcccca gcagcggaat aaataaagaa atctgtttca ttgtccctca
     1381 cctttctcat cgcagttcac aaaatcattc ttcaaatccc tgaccgtaca ggtttttaca
     1441 atgtcaggat tttcaaaaac cgtattccgt gatgatttat aaacatatgc cacaatgcgt
     1501 ttcccacccg catcaggttc tggtgcactg acttcacata acggaggtgt tattccggac
     1561 tcatacagca ccggataatt tatttttttg acgctgccat cagaaaccag cttattcatc
     1621 gaagggtt
//
LOCUS       DAZUCC010000441         1601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZUCC010000441 DAZUCC010000000
VERSION     DAZUCC010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.443"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..580
                     /locus_tag="ACHY09_005225"
     CDS             <1..580
                     /locus_tag="ACHY09_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3888033.1"
                     /translation="FATIGMDNPAQAIRNARENIKKSSSVRATFGSYEAALDDVEAER
                     IIRTSPKFIDDYYWGWTAAEKKAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDV
                     VREFIYWDWLYEMRQTAGRETGDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAV
                     EVCRWFLASGKDEYMEDNGSAVILNLVGECEQ"
     gene            659..1084
                     /locus_tag="ACHY09_005226"
     CDS             659..1084
                     /locus_tag="ACHY09_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFS3888034.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIELEMDRYVERERSGAQVSQFSGHTGN"
     gene            1284..1440
                     /locus_tag="ACHY09_005227"
                     /pseudo
     CDS             1284..1440
                     /locus_tag="ACHY09_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
BASE COUNT          425 a          326 c          463 g          387 t
ORIGIN      
        1 tttcgcaacc ataggcatgg ataacccggc gcaggcgatc aggaatgccc gtgagaatat
       61 caaaaaatcc tcatcagtca gggccacatt tggcagctat gaggcagcac tcgacgatgt
      121 ggaggctgag cggataattc gcacctcccc gaaatttatc gacgattact actggggctg
      181 gactgcggca gagaaaaaag ccggaagtat tgacggtgtt cgctcaaatg aaattgatga
      241 tcagcgtcgt gcatatgttg atggctatcg tgatgtactg ccagagcctc atacattgtc
      301 agacgttgtt cgtgagttta tttactggga ctggctttat gagatgcgcc agacagcggg
      361 gagagaaaca ggggacaaat acggtttcac aggtgaacat catgagtcag tatatgatcg
      421 gcagttctgg ctcgaaaatt tgctgggaaa aataaagcct gtgacgcgtg atgaagcggt
      481 tgaagtgtgt cgctggtttc tggcaagcgg aaaagatgag tacatggaag acaatggttc
      541 agcggtcatt ctcaatctgg ttggggagtg tgagcaatga agccggagat atccagtaga
      601 cattttcccc ctgagcattc aaatcagaaa aagtatggtt acagcggagt acagaataat
      661 gcgtgatatc caacaggttc tgtcccgatg gggtgcatgg gtggtaaata atcatgagag
      721 tgtggcatgg tccggcgtcg ctgccggatt taagggagtg atcccatcaa aggttaaatc
      781 acggcctcag tgtaccgatg atgatgcgct gattatcagc aattgcatgg cgcaactgaa
      841 cgtcaacaac agcgatttgc atgattttct gtatgattat tatgtgttcg ggatgacgct
      901 tatgggactg ggccgtaagc atgagcgctc tgattgctgg gccgggcggg tactgcaaaa
      961 agctgaaggt gttattgagg ggatgttgat tatgcaggga atagaactgg aaatggacag
     1021 atacgttgag cgtgaacgat caggggcaca ggtcagtcag ttttccggac atacgggaaa
     1081 ttgaaagcgc ggggttttac tgtagaatga ctgcgggtgc ttgagggtgt ctgcctcggg
     1141 catgctgccg taaggcagac ggagaaaagc cccagttaac attacgtgtc ctgcaagacg
     1201 cttaacatta atctgaggcc caatctatgt cacacaaccg taggttagcc tcttacgtgc
     1261 cggaaggcaa ggagaagcag tctatgaagc agcaaaaggc gatgttaatt cgccctgatc
     1321 gtcatctgtt taactgtcat agtgacggca ctggtaacga ggaaagacct ctgcgaggta
     1381 cggatccgaa ccggccagac ggaggtcgct gtcttcgtag actacgaatc tgagaagtaa
     1441 gagaccaggc gggggagtaa tctcccgcca cctctgatgt gtcaggcatc ctcaacgcac
     1501 ccgtacttta tttgtcatat gctccgcaaa atgcaggttg tcgttgcaac ccgtgcggta
     1561 attttttgtg tgcgagtgct caaaaaacgt tgattttcat a
//
LOCUS       DAZUCC010000442         1577 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZUCC010000442 DAZUCC010000000
VERSION     DAZUCC010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.444"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            163..453
                     /locus_tag="ACHY09_005228"
     CDS             163..453
                     /locus_tag="ACHY09_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888035.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            complement(445..723)
                     /locus_tag="ACHY09_005229"
     CDS             complement(445..723)
                     /locus_tag="ACHY09_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888036.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRASLAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            844..1266
                     /locus_tag="ACHY09_005230"
     CDS             844..1266
                     /locus_tag="ACHY09_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFS3888037.1"
                     /translation="MRSAVARLLLIPLITATIVLTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGVKDKAAGVSAETAQQRIKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
BASE COUNT          489 a          385 c          328 g          375 t
ORIGIN      
        1 aaaacctact actcaccatt gtccatgtca agagcatgga ttttcatttt tgcaataagg
       61 atcacactat ggggaggcag gcattgagaa cgtcgaaaca gaacaccgga gcaaatcagg
      121 atgagatata aaactgttgg atcatgaaaa acggagaacg atgtgagcaa atcaccccgc
      181 cataaactga acaaaacaga caaacgactt ctcgacaccc ttgttgctgc cggatatgag
      241 catgacaaag cccgtgacct catccagaaa caggtttaca cgctgacact ggctgatcag
      301 cgccatgtgg tcagtgaaat cagtaacggt gtgaatccca cccaggctta ctcggcggta
      361 taccaggcaa gacgcattcg cctcgcccgt aaatatctga acggtaaaaa ggttatggaa
      421 gaaaccgggg aaaatacgcc cccatcagcg taaggatttc ttttgccgct ccagagactc
      481 cagtttttta cgcaaatcct ctcttttttg ggcatctctg gtgccagcca gagatgccct
      541 caactcatcg atctgaagtt gtatcttcag tctgttactg aacattttct gtctggcatt
      601 aacatccgca acaatgccat tttttgtctt ctcggccttt tgttgaaaaa cattgctgtc
      661 cgcatgactg gcaaccgaag cagaaagaac actaaaaagc aggactggca cacatttttt
      721 cacgggatta ttcctgactc attgaccatc aaatcacatt ggggtaaacc gacgtatgat
      781 aagagatact cttcggaaat ataactccct gagtatcaag attaaaaacg caaggagatg
      841 tttatgagat ctgccgttgc caggctgctt ctgatacctc tgataacagc aacaatcgtt
      901 cttacaggat gcacaccaaa gaccagcctg gaacgacata cccggcatta tgtttatgct
      961 tcagatgatg gatttgatcc taacttctac acccagaaag cagacaccat acgtatgatg
     1021 ctcccgttct ttcagcagtt ccgggatatg ggggtgaaag acaaagcagc cggagtatca
     1081 gcagaaacgg cacagcaacg tataaaagaa ttccactcag aaaaattttt tcactcactc
     1141 cggagcacaa caacctttgc tggcagaaaa tacacaaaca gcgatatgcc gtcgccgaaa
     1201 aaaatgaaac taatggcaga caccatttct gcggtttatc tcgatggata cgagggcaga
     1261 cagtaagggg atttaccata atcccttaat tgtacgtacc gctgaaacgc tctcatacgc
     1321 gttctgacgc aaaaacgcaa aaagacaagg aaaccccagg ctaaaccgaa aaaatgcgtc
     1381 agaatcgttt tcaggtactt gcataactat tcacaaaagc gatgcgcgat cgatttcgac
     1441 tattccgacg gacgaacagg ttaaaatgat aagtttcctc cctccggatg gaaggttcag
     1501 cacggggatt tttaccccct tccatcaacc tgaatatgca tctgggagta cccattagtt
     1561 tctactcaaa cactatc
//
LOCUS       DAZUCC010000443         1548 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZUCC010000443 DAZUCC010000000
VERSION     DAZUCC010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.445"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..929
                     /gene="yfbL"
                     /locus_tag="ACHY09_005231"
     CDS             12..929
                     /gene="yfbL"
                     /locus_tag="ACHY09_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFS3888038.1"
                     /translation="MIFYQPWVRALPPTPRHASPEQLEKTVRYLTQTVHPRSADNIDN
                     LNRSAEYIKEVFISSGARVTAQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSVSSY
                     ENDQLTYTPGADDNASGVAGLLELARLLQLQTPGIGVQLVAYASEEPPFFRSDEMGSA
                     VHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLYPDRGDFIAVVGRMQDINA
                     VRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYH
                     LPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            1032..1535
                     /gene="yfbM"
                     /locus_tag="ACHY09_005232"
     CDS             1032..1535
                     /gene="yfbM"
                     /locus_tag="ACHY09_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFS3888039.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
BASE COUNT          420 a          335 c          376 g          417 t
ORIGIN      
        1 cgcttacttt tattattttt taccaaccct gggtcagggc attaccacca acgccccgac
       61 acgctagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      121 gtagcgccga caatattgat aatctgaata ggtccgcaga gtatataaaa gaggtcttta
      181 tcagtagcgg tgccagagtt accgcgcagg atgtccccat tacgggtggc ccctacaaaa
      241 acattgttgc tgattatggt cctgccgatg gaccgctgat tattattggt gcgcactatg
      301 actctgtcag tagttatgaa aacgatcaat tgacctatac gccgggcgcg gatgataacg
      361 ccagcggcgt ggcaggatta ctcgaactgg cgcgtttgtt acagctgcag actccaggga
      421 ttggcgtcca actggtcgcc tatgcgtcgg aagaaccgcc cttctttcgt agcgatgaaa
      481 tggggagcgc ggtacatgca gcttcgcttg agcgtccagt gaaattaatg atagcgctgg
      541 agatgattgg ttattacgac tcaacgcctg gtagccagga ttacccatcc ccggcaatgt
      601 cctggcttta tcccgatcgg ggagacttta ttgccgtggt cggcagaatg caggatatca
      661 acgccgttcg tcaggtaaaa gcggcattgt tgtcatcccg ggatttatct gtttattcta
      721 tgaatgcccc aggttttatt cccggtattg atttctctga tcaccttaat tattggcaac
      781 acgatattcc cgccgtaatg attactgaca ccgcttttta tcgtaataaa caataccact
      841 tgcccggtga tatcgcagac agattaaatt atcagaaaat ggctcaggta gtagatggag
      901 ttacaacctt gttatacaac agtaaataat aaaaatttct cgaggcggat ccagaaaaag
      961 caacatgcgc attttatttt ctttcgtctg ttttaagatg cgaaacatga aattcaacgg
     1021 aggaaaaata aatgggaatg attggctatt ttgcagaaat tgactcagaa aagattaacc
     1081 aactgcttga gtctactaaa aaacctttga tggataatat acatgacacg ctttcaggat
     1141 tacgccgact ggatattgat aaacgctggg attttttaca ctttggcctg acgggtacat
     1201 ctgcatttga ccccgcgaag aatgacccgc taagtcgcgc agtgcttggt gaacacagcc
     1261 tagaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
     1321 tcgaccgact ggaagctctg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
     1381 tcaatgaaat ggagatttat ccgggagtaa ccttttcaga agagttagag gggcaactct
     1441 ttgcttcgat catgcttgat atggaaaagc tgattagcgc gtatcgacgt atgctgcgac
     1501 aagggaatca tgcgctgacg gttattgttg ggtgatggtg tcagggca
//
LOCUS       DAZUCC010000444         1535 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZUCC010000444 DAZUCC010000000
VERSION     DAZUCC010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.446"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            282..905
                     /gene="rcsA"
                     /locus_tag="ACHY09_005233"
     CDS             282..905
                     /gene="rcsA"
                     /locus_tag="ACHY09_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFS3888040.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(949..1137)
                     /gene="dsrB"
                     /locus_tag="ACHY09_005234"
     CDS             complement(949..1137)
                     /gene="dsrB"
                     /locus_tag="ACHY09_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFS3888041.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            1300..1527
                     /gene="yodD"
                     /locus_tag="ACHY09_005235"
     CDS             1300..1527
                     /gene="yodD"
                     /locus_tag="ACHY09_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFS3888042.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHL
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
BASE COUNT          447 a          337 c          332 g          419 t
ORIGIN      
        1 cgtttatcat gctaccctaa ggattatccg aaaaataata cctacgaaca tcttccagga
       61 tactcctgcg gcgaaatatt tgttttaagc tcactcacat atcgcaacat ttactttact
      121 ttaagacaat tccaggcaaa ttatacaaca ctttacggga tagtaagtcc gcctgaaaaa
      181 tcgcgagagt ggcgcattag gtgacccatg ttgttccgtt tagtcatgat gaaatattca
      241 ggtaagggga attatcgtta cgcattgagt gagggtatgc catgtcaacg attattatgg
      301 atttatgtag ttacacccga ctaggtttaa ccgggtatct gttgagtaga ggggttaaaa
      361 aaagagaaat caacgacatt gaaaccgttg atgaccttgc catagcttgt gattcacagc
      421 gcccttcagt ggtgtttatt aatgaggact gtttcatcca cgatgcttct aacagtcagc
      481 gcatcaagca catcattaat caacatccca atacgttatt tatcgttttt atggcaattg
      541 ccaatgttca ttttgatgaa tatctattgg tcagaaaaaa tttattgatc agttctaaat
      601 cgattaaacc ggaatctctc gatgatatcc ttggcgatat tctgaaaaaa gagacaacga
      661 taacctcgtt tttaaatatg ccgacgttat cattgagccg aaccgaatcg agtatgttgc
      721 gaatgtggat ggcaggtcag ggaaccattc aaatctccga ccaaatgaat atcaaagcca
      781 agaccgtttc atcgcataaa ggtaatatta aacgtaagat caaaacgcat aataaacagg
      841 ttatctacca tgtcgtccga ctgacggata atgtgactaa tggtattttt gtcaacatgc
      901 gctaacacat tctgactggt ggtttcccac cagtcaggct gaattagatt actctgcttt
      961 ctccacaaag ataccgtcct gatgccctgc ttcattaaag aaccagatgc cgagcgggta
     1021 gtcttccagc gaaaccaggt acattgtgcc ttcactaaac tcctcaactg ccagtaccac
     1081 gccaggacga cgcggaccgc catccgtttt gactgttacc cgatcattca ccttcatcgt
     1141 tttcctcctg tggctttgtg ccagtgtaga acaatttcgt ttttctggca gcgccaggcg
     1201 cgcgcgagtg ctgattttct cgacggtcta tacttaagag atgccaggcg gacttaacga
     1261 ctggcggcaa caacagagta acggttgcga ggaaagatga tgaaaaccgc aaaagagtac
     1321 agcgataccg caaaacgtga ggtcagcgtc gatgtcgatg ccctgctggc ggcgatcaat
     1381 gaaattagcg aaagcgaagt tcatcgcagc cagaacgatt ctgaacacct tagtgtcgat
     1441 ggacgtgaat atcatacatg gcgtgaattg gcggatgcct tcgaactgga tattcatgac
     1501 ttcagcgtct ctgaagtgaa tcgttgaatg cacaa
//
LOCUS       DAZUCC010000445         1516 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZUCC010000445 DAZUCC010000000
VERSION     DAZUCC010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.447"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(57..1481)
                     /locus_tag="ACHY09_005236"
     CDS             complement(57..1481)
                     /locus_tag="ACHY09_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS3888043.1"
                     /translation="MFQRSKYDSAYVYALCCVAALAGLMFGYSTAVITGVILPLQQYY
                     QLTSTETGWAVSSIVIGCIIGALVGGKIADKLGRKPALLIIAVIFIVSSLWGAMSESF
                     MVFSISRIVSGFAVGMAGTASTMYMSELAPADIRGKALGIYNISVVSGQVIVFIVNYV
                     IAKGMPSDVLVEEGWKSMLFAQVIPSVTMLVITLFLPESPAWCARNNKGEKRSLKVLA
                     RIYRGVSATDVAAIFDSMRETEARENAAGREFVILKSSPVLRYILLVGCCIAVLQQFT
                     GVNVMNYYAPLVLQNSSTEEVMFQTIFIAVCNVIGSFIGMILFDRYGRIPIMKIGTIG
                     SIIGLLIASYGLYTHDTGYITIFGILFFMLLFAVSWSVGAWVLISEVFPEKIKGFGMG
                     LAVSLMWIANFLISLLFPVINDNTWLQETFGGAFSMWIFVVFNLVCYVFISRYVPETK
                     GIPLTQIEQLAGNKLREIQGKAMM"
BASE COUNT          502 a          410 c          261 g          343 t
ORIGIN      
        1 cggtaagatg ctcaacatta ccgggcatta ctgctgattg tgacacaatc agactattac
       61 atcatagctt taccctgaat ttcacgcagt ttatttccgg caagctgctc aatttgtgtt
      121 agcgggatcc cctttgtttc aggaacatag cgagaaataa aaacataaca aaccagatta
      181 aagacaacaa aaatccacat agaaaacgct ccgccaaagg tttcctgcag ccaggtatta
      241 tcatttatta ccggaaacaa cagtgatatt agaaaattcg caatccacat taaactaacc
      301 gccagcccca tgccgaatcc tttaatcttt tcgggaaaca cctcagaaat cagtacccat
      361 gctcctacgc tccagctgac agcaaacaac agcataaaaa ataaaatccc aaaaatggtg
      421 atatagccag tatcatgggt atacaaacca tatgatgcga tcaacaaacc aataattgac
      481 ccgatagtgc caatcttcat gattggtatt cgtccatagc gatcgaacaa aatcatcccg
      541 ataaaactac cgatcacatt acaaacagca ataaaaatcg tctggaacat cacttcctct
      601 gtactgctgt tttgtagcac cagaggtgcg tagtagttca tcacattgac accagtaaac
      661 tgttgtaaaa cagcgataca acagccaacc agcagaatat agcgcaacac aggagagctt
      721 ttcaggataa cgaattccct gcctgcagca ttctcccggg cctccgtttc cctcatacta
      781 tcaaaaatag cggctacatc ggtagcgcta accccccggt agattctggc aagcactttc
      841 agagaacgtt tttccccctt gttattacgg gcacaccatg ccggcgattc aggcaaaaat
      901 aaagttatca ctaacattgt aacagaaggt atcacctgag cgaataacat gctcttccag
      961 ccttcttcga ccagcacatc agagggcatc cccttagcaa tcacataatt aacaataaaa
     1021 acaatcacct gaccagaaac tactgatata ttgtaaatac ccagagcttt accacgaatg
     1081 tcagctggtg ccagttcaga catatacatg gtagatgcag tccccgccat cccaacagca
     1141 aaaccactga cgatgcgcga aatcgagaaa accataaaag attcactcat cgccccccac
     1201 agtgacgaga cgataaaaat aactgcaata atcagaagcg caggcttacg ccccaactta
     1261 tcggcaattt tccccccgac cagcgcacca atgatacaac caatcacaat gctcgatact
     1321 gcccacccag tttcagttga agtgagctgg taatattgct gtaaaggcag tatcaccccc
     1381 gttatcactg ccgtcgaata gccaaacatc aatccagcca gtgcagcaac gcagcacaat
     1441 gcgtacacat aggctgaatc atacttgctt ctctgaaaca taccccctcc ctgcctgttg
     1501 aggaaatgac atacga
//
LOCUS       DAZUCC010000446         1514 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZUCC010000446 DAZUCC010000000
VERSION     DAZUCC010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.448"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1514
                     /gene="cba"
                     /locus_tag="ACHY09_005237"
     CDS             <1..>1514
                     /gene="cba"
                     /locus_tag="ACHY09_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin B"
                     /protein_id="HFS3888044.1"
                     /translation="GSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPSMPPGW
                     GDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAARTVLE
                     NGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGNNV
                     PVKYRLTDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNLP
                     DLVLSVGQMNTPVRSNPEIQEDGVISETGNYVEAGYTMSSNNHDVIVRFPEGSGVSPL
                     YISAVEILDSNSLSQRQEAENNAKDDFRVKKEQENDEKTVLTKTSEVIISVGDKVGEY
                     LGDKYKALSREIAENINNFQEKTIRSYDDAMSSINKLMANPSLKINATDKEAIVNAWK
                     AFNAEDMGNKFAALGKTFKAADYTIKANNIREKSIEGYQTGNWGPLMLEVESWVISGM
                     ASAVALSLFSFTLGSALIAFGLSATVVGFVGVVIAGAIGAFIDDKFVDELNHKI"
BASE COUNT          418 a          289 c          400 g          407 t
ORIGIN      
        1 ggtagtgtac cgacagaagg tattgattac ggggacacaa tggttgtgtg gccgtcaaca
       61 ggacgaattc cgggcggtga tgtgaaaccc ggaggctcat caggtctcgc tccatccatg
      121 cctccgggat ggggggatta cagcccacaa ggtatcgcac ttgtacaaag tgttcttttt
      181 cctggaatta ttcgccggat tattcttgat aaggaacttg aagagggaga ctggtcggga
      241 tggtctgtca gtgtgcatag cccctgggga aacgagaaag tttccgctgc acgaacagtt
      301 cttgaaaatg gtttacgtgg tggtttgcca gaaccgtctc gcccggctgc tgtttctttt
      361 gcccgtctgg agcctgcttc cggaaatgag caaaaaatta ttcgtcttat ggttacacag
      421 caactggagc aggtaacgga tatccctgcc agccagttac cagcagcggg taataatgta
      481 ccggtaaaat atcgtctgac ggaccttatg cagaatggta cacaatatat ggctattatc
      541 ggaggtattc cgatgacagt gccagtagtg gatgccgttc cagttccgga ccggagtcgt
      601 ccgggaacca atattaaaga tgtttacagt gcccctgtat caccaaatct accggacctg
      661 gtattaagtg tgggtcagat gaatactcca gttcggtcta atcccgaaat ccaggaagat
      721 ggcgttattt ctgagacagg gaattatgtt gaggctggtt atacgatgtc cagtaataat
      781 catgatgtca ttgtccgttt tcctgaaggc agtggagttt ctccgctata tatttcagcc
      841 gtggagattc tggacagtaa tagtttaagc cagcgccagg aagccgaaaa taacgcaaag
      901 gatgacttca gagtcaagaa agaacaagaa aatgatgaga agacggtcct gacaaaaacc
      961 agcgaggtca tcattagtgt cggtgacaaa gtcggggaat atcttggaga taaatacaag
     1021 gcgctttccc gtgaaattgc agagaatata aataattttc aggaaaaaac gattcgtagt
     1081 tatgatgatg caatgtcttc cattaataag ttaatggcta accccagcct taaaataaat
     1141 gcaacggaca aagaagccat tgtgaatgcg tggaaagcat ttaatgctga ggatatgggg
     1201 aataaatttg ctgcgttggg taagacgttc aaagcagcag attatacaat aaaggcaaac
     1261 aacatcaggg agaagagtat tgagggttac cagactggta actgggggcc attaatgctg
     1321 gaagtcgagt cctgggttat cagtgggatg gcatctgctg tagctcttag tttgttttct
     1381 ttcacattag gctcggccct tatagccttt ggtctttcgg ccacagttgt tggttttgtt
     1441 ggcgtagtta ttgcaggtgc tattggtgca tttatcgatg ataaatttgt tgatgagttg
     1501 aatcacaaga tcat
//
LOCUS       DAZUCC010000447         1488 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZUCC010000447 DAZUCC010000000
VERSION     DAZUCC010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.450"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /locus_tag="ACHY09_005238"
     CDS             complement(<1..353)
                     /locus_tag="ACHY09_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS3888045.1"
                     /translation="MTTFSHSSTTPSVSVTTASGSDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIA"
     gene            complement(444..1262)
                     /locus_tag="ACHY09_005239"
     CDS             complement(444..1262)
                     /locus_tag="ACHY09_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS3888046.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT          337 a          388 c          420 g          343 t
ORIGIN      
        1 gcaatgccgg cggcttccgg gctcatttca gcgcggttac cgttcatggc attgaacagt
       61 acccagtttt cgccatcatc gtcatccggt tcgggggcca taaatgcgcc accgttgttc
      121 agtgtgtaca gattccagat accaccgcag tagtcttcgc aaagacggtc catccagccg
      181 aagatacggg gctccagggt cacccactgt ggaatgaggc caaaatgctg cggccagaag
      241 ctgatgcgct gttcatcagg gactatggtg gcaaccagct gaggctggtc actccctgat
      301 gcagttgtta cggaaacaga aggtgtggtg gagctatgcg aaaaggttgt catgaaatga
      361 ttccttataa aaagcaaatg aatgaagaag actcgggggc agggacagac gtgagtcagg
      421 acggcgcttt caggtaaacg gtatcagcgc atgctctcca gcagcgtttc cgccatcacc
      481 cacaatgcgc gattgagctt gatgtcggtg tcgatactgt gaatggcacg ggtatggata
      541 cgttttcctt ttgcactacg gccggaaatc ccgcctttca gcatattctc ctggatggtc
      601 tgatatgcgc tccacaggtc cttaccgtaa tcctcccggc gtcgtggtgt cagaatgtcg
      661 gcagtggtga cgggttgatg ttcatcacca taacggtaag tcagcgccgc ctgtgccagt
      721 gcctggcgtg ccggtggcgg cagaaccaga gactgcatgg catcacgctt ttcctcaatc
      781 cggtcaaaaa cgcccactac ctcgtaagcc ccttcaatga ctctgtccac cacatttccc
      841 cggtggggca cacggacttc ccccagagac tgaccgcaga cgcagccgtt ctggcagacg
      901 aatctgaagt aacccggcag catctggtag ctggaggtac cgtcatggga gttgagcaga
      961 ataatttcag gcacatgctg accggttatc tgtccggccc gccgcagacg cagcatgtgt
     1021 ttggtatatt cccggcggcc cgggtcacgt acgcgggtct ggcaggcgaa gaatggctga
     1081 aagccttccc gctgcaggct ttccagcacg gtgatggtgg gaatccaggt atagttctcg
     1141 cttctggatg tgtgtttctc ttccccgaat acgctgggga cgacctgcat cagttcttca
     1201 cgagttaagg ggcggtcacg gcgtatctgg ttcgcataac caaaacgact ggctaatcgc
     1261 atgatttatt ccttatgaat gagttagcgg tataaatgaa aaagccgcgt ctcccggaga
     1321 agacgcggcc tgatggggga aatgaatgac gtttattgtc tgagaagccc ttcactggcg
     1381 agctgcgtat ttttatggaa ggagatcacc caataacccc atcgggatcg ggtaataacc
     1441 ggtcagactg tgatcgattc agatgccatt tggcagcaaa agagcctt
//
LOCUS       DAZUCC010000448         1474 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZUCC010000448 DAZUCC010000000
VERSION     DAZUCC010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.451"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..287
                     /locus_tag="ACHY09_005240"
     CDS             <1..287
                     /locus_tag="ACHY09_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFS3888047.1"
                     /translation="HCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQ
                     KLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSASGKK"
     gene            complement(369..711)
                     /locus_tag="ACHY09_005241"
                     /pseudo
     CDS             complement(369..711)
                     /locus_tag="ACHY09_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            724..1319
                     /locus_tag="ACHY09_005242"
                     /pseudo
     CDS             724..1319
                     /locus_tag="ACHY09_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          429 a          359 c          338 g          348 t
ORIGIN      
        1 ttcactgcct gcctggaaat atatctacaa aaagctgtgt aatacgcaat gcggcgcagg
       61 ccttaactga cgatggaact ctatacggag ccacaattct tggtgacggc gttgtgcaca
      121 atagcttcgg tcaaaaactg atgcgcattt acaatcagaa aggcatcttt tcaaacacaa
      181 aagattccga agaaggctta acacatatac tctcagagca tttcgagaat gttaaaacca
      241 aggttaaagg tactgtagta atgttttccg cttcagggaa aaaatagcat ccagccgcag
      301 cacgttcttg cgcacgacgt tctgcggcat aatcccaatg attactctct gacagggttc
      361 acaggccact aaatatcagg ggcagttgat gtatcaactc ggttcagcaa cacccgatac
      421 atcttccagg cttccagcaa cgaggtttct tcctccgtat cctttatcca tgatatgccg
      481 ttccacttct gaaactcccc tgccggggat acctggtgac attttccggt aacggaccga
      541 gttcagaaat aaataatgcg tcccctgatg ccacgtcata aaccgtttta ccccgatggt
      601 cttcaacgag atgccacgat gcctcatcac tgttgaaaac aaccacgaag ccagccggaa
      661 tatctgggca atatcagtac tgtttgcagg caaacctgta tgaggtggaa tggcacaaga
      721 gacttacaaa agaatcaggc caaacgcctg attgatgaga gccccgaacc actgaaatct
      781 gtcgtcaaat ttgcgctggt aactgttctg agaaagtcga acatcataaa tctggagtgg
      841 caacagattg acatgcagcg acaagttgcc agggtgaatc cagaagatag caaatcaaac
      901 tgcgccattg gtgtggcgct gaacgatacc gcctctaaag tgttgcgtga tcaaacaggc
      961 aagcatcaca aatgtgggtg tacataccag ggcggctaag cgagcagatg gaacatcaat
     1021 gcctgcgctc aggaagatgc gcattgacag caagacagca tggctatcag cttgtcgtcg
     1081 tgcaggaatt gaaaatttcc gtttccatga cctctggcat acctgggcaa gctggttgat
     1141 tccgtcaggc gtcccattat cagtgcttca ggaaatgggc ggatgggagt ccagagaaat
     1201 ggttcgtaga tatgctcacc ttgcgcctaa tcatttgact gagcacgcga ggaaaataga
     1261 cgacattttt ggtgataatg tcccactctg gaattataga agaaataaag aaggcgtaac
     1321 tgattgaatt ttaatggcgc gccctgcagg attcgaacct gcggcccacg acttagaagt
     1381 tcctagaacg acattttaag tcaacaactt accgcgccat ctctgcgctc acacgtccca
     1441 ctacctcaaa acatgtaaag ccttgcaagc catt
//
LOCUS       DAZUCC010000449         1468 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZUCC010000449 DAZUCC010000000
VERSION     DAZUCC010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.452"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            622..830
                     /locus_tag="ACHY09_005243"
                     /pseudo
     CDS             622..830
                     /locus_tag="ACHY09_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            817..1006
                     /gene="tnpB"
                     /locus_tag="ACHY09_005244"
                     /pseudo
     CDS             817..1006
                     /gene="tnpB"
                     /locus_tag="ACHY09_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1009..1197)
                     /locus_tag="ACHY09_005245"
     CDS             complement(1009..1197)
                     /locus_tag="ACHY09_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFS3888048.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
BASE COUNT          391 a          359 c          291 g          427 t
ORIGIN      
        1 aaaaaattat cactatgcaa agatgtaaca aaatattgct cagtgatcta tattgttgtt
       61 tttatttgag gttttatatt ttctagtgta tttgactttt ctttctcaag tgttatttct
      121 tgacctttat cacagaacca tctccctgga tatccccatc acattgcatg aaaagcaagt
      181 tttaccaatc ttgattttca gtgttaaata agattaataa ctgtaattct gcaaaaaaaa
      241 gaacaccata tgtagtatga ctacgtattc cactctgttc gttcacggac tggcatcgaa
      301 gtctgaggtg tactggcaat agcggataca gtcatttgtt ctttttttaa gcattcatcc
      361 gatgatactt ttcctgaatg ctaccggagt ggtattcccc tgccgtgagg gagggcgctg
      421 acggttataa aaatcctgat atatacccat tcccgtatga cagcgatggc gtcacatctc
      481 tgttctacca tcttcacaga aatatttcgt tcattagcat tccggtattc gtataaccac
      541 cttcggtgga gaaagaataa acaatccctg gaatgcgatg agcgcttgac cagaaaagca
      601 tcatattgct tcatggcgca tcagcgacca caggatgccg caaaagccga aaaacggacc
      661 agagcgcctg caatcattcc tcgcctcagt atctgttgcc catggagtgg ccgataacag
      721 atcattgtcg gatgagcctt atccccttcc ttcccggtgg tatccggatg tgctgccagc
      781 cttctcagtt aaccgatgtt ttccgggctt tttgccatac tgaggactga taacgtattc
      841 ctggtcgtaa aacccgtcaa tatgcgccgg ggcatcgaca aaactgacac agtacgtccg
      901 gaaagaactt aacgctgcat ggcacgacaa tgctgccttt gtctttacca acaaggctcg
      961 ttcccgcata aaagttctac gccgggataa acatggcgta tggctgcgtc aggccttcac
     1021 tacttcacac cggaagtctt ccaccctgac acgaatatac cgtccgggct ctttcgcagg
     1081 tgcaaacagt ttttcacatt cggcgaatgt catttctgaa tcctggtaac gcaaccactg
     1141 aaccggcata ttaccgggtt tgattattgt cgcagttatt ttatatcgtt tccccatatc
     1201 gcctctcatc ctgacaaaaa aattaccggc tgagccatca ctccggtaaa gcatgcgaaa
     1261 aaaagtgagc gtctcagcaa tggctcatgc gatgattctg tacgggcacc gatggcacct
     1321 cagaatacaa caaagatatc agagtaacat gtctctctcg ttatttttca gagttcttga
     1381 caccctcctt ctgccacatt gtcaacgcca gtggagactt ctgctgtcag ttttttcatc
     1441 catacagagt gacattaatt atcctgaa
//
LOCUS       DAZUCC010000450         1445 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZUCC010000450 DAZUCC010000000
VERSION     DAZUCC010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.453"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            50..454
                     /gene="stpA"
                     /locus_tag="ACHY09_005246"
     CDS             50..454
                     /gene="stpA"
                     /locus_tag="ACHY09_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFS3888049.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(501..1025)
                     /gene="ygaP"
                     /locus_tag="ACHY09_005247"
     CDS             complement(501..1025)
                     /gene="ygaP"
                     /locus_tag="ACHY09_005247"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFS3888050.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1035..1334)
                     /locus_tag="ACHY09_005248"
     CDS             complement(1035..1334)
                     /locus_tag="ACHY09_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS3888051.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          397 a          341 c          340 g          367 t
ORIGIN      
        1 tacgcgacga aatacttttt tgttttggcg ttaaaaggtt ttctttatta tgtccgtaat
       61 gttacaaagt ttaaataaca ttcgcaccct ccgtgcgatg gctcgcgaat tctccattga
      121 cgttcttgaa gaaatgctcg aaaaattcag ggttgtcact aaagaaagac gtgaagaaga
      181 agaacaacag cagcgtgaac tggcagagcg ccaggaaaaa attagcacct ggctggagct
      241 gatgaaagct gacggaatta acccggaaga gttattgggt aatagctctg ctgcggcacc
      301 acgcgctggt aaaaaacgcc agccgcgtcc ggcgaaatat aaattcatcg atgttaacgg
      361 tgaaactaaa acctggaccg gtcagggccg tacaccgaag ccaatcgctc aggcgctggc
      421 agaaggtaaa tctctcgacg attttctgat ctaattactg aggccggatg ttgctgctat
      481 ggcgcgtccg gcgtcgcttc tcaagctcgt tggttccacg gcatcttatc taacaacctt
      541 gccatcccac aaaaaccgct aattcctgca aacagtaacc cggcaccaac aaagccgctt
      601 aataagaaga aaccgctatt tacggtataa cctagtataa cgccgattaa tattaaacca
      661 cccgcagcga tctgcacctg gcgcattaac ggcaagggtt gagatttatt taccgctact
      721 ggtaatcctg cttttttcca gccatcaatc ccatcttcga gtaaaaagat ttctgcgggg
      781 gcggcaatcg ctgctaattt atcggcgtta ttactggtgc gtttaccagc ctggcagtgg
      841 aatattattt gctcgcgacg taatttagcc ggaagacctg actgttccag cacggataat
      901 ggagccagat ctgcttcagg aatatgttca cgaagatatt catcagcgtc acgaatatcg
      961 attaactttg cgccgcgtgc gattaattct tgtgcatcat gcggcgaaat ggttgtcaaa
     1021 gccatagtga ctccttacgg acaatagaca tttttcaggg tggcgataat ggcatttacc
     1081 gcctcatttt taatggaata tagaatgcgt tgggcatccc gttggctgtc gataagccct
     1141 tcgtcccgca tacgagcgag atgctgtgat gttgcagagg cactcagtcc ggtaatgcgc
     1201 gtcagctctc ccgcactggt gccgggggaa ccgctaagca tgcacagaat cagcaaccgt
     1261 ttagggtggc tcattgcttt caataaggcc gctgcctgtt cggcactagc ctgtaattgc
     1321 gcgagttcag tcatagatat ttaagtattt tctaaattaa gtaaactcta aactaaaaat
     1381 gcaacatata ccagcctcag cagcgtaaat gagagtaaaa gcgtaagctg aaactggcag
     1441 gctcc
//
LOCUS       DAZUCC010000451         1431 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZUCC010000451 DAZUCC010000000
VERSION     DAZUCC010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.454"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..607)
                     /locus_tag="ACHY09_005249"
     CDS             complement(<1..607)
                     /locus_tag="ACHY09_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3888052.1"
                     /translation="MQHLPAPIHHARDVAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMH"
     gene            complement(609..1022)
                     /locus_tag="ACHY09_005250"
     CDS             complement(609..1022)
                     /locus_tag="ACHY09_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888053.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
BASE COUNT          306 a          423 c          394 g          308 t
ORIGIN      
        1 tgtgcatcgc ataggaatgg cggaacgtat gtggcgtgac cgggacagag aacgtcacac
       61 catcagcggc agcggcggca accgcttccc caatccaggt cctgaccgtt ctgtccgtca
      121 cttcccagat ccgcgctttc tctgtcctgc ctgttcgttt attacgccgt tccatgggga
      181 ttttcagtgt ggccaccatc gtctgcagct ggctgacgta ccaggagtcg gagagcggaa
      241 ccagccggtg agtctgctga ccggctggca ttcttcctgc cgtcctggcg gctttttctg
      301 tccgctgctt cagagtagcc agctgcacaa acggatacgg gggcgtgagc gaaaaatccc
      361 cccgcgtcag cgccagtgct tcattaatgc gcgcaccggt gttccacagt gtggccagca
      421 gcatcttgcg gcgcagatcc gggacgtaat ggagcagggc gctcacttca ggggccagca
      481 ggtattttgg cagttcatca tgaaccatcg acatctggcg gagtgccagc gctgccggat
      541 aatcgatggc aacaggaagc tgagcgacat cccgggcatg gtggatcggt gccggcaggt
      601 gctgcatatc agtgttcact ccacggggaa acgtcgggca ggttttccac aacacggtac
      661 tttcccggat aacggagctg gttaccgcca cgcgtccaga cggtgacaac aaaaccccct
      721 ccctggttgt tttcggggag caggcgatcg cactgctcac tcagcatgtg gatcacaaac
      781 ggcgcatcgc tgccgtcttc aaataccagt tccagcgcat cgcggccatg cagcgttccc
      841 ttactgatga tgacgtactc agccccccgc atctccctga gtaaatgttt tgccgcatcc
      901 gggacaagca gcctggctgc gccggcattc cagctgaggt agacatatcc ggcctgcgcc
      961 tgtacagact gccagtaatc cgttccggcg acagcctgac cttcattctc aataaaaaac
     1021 atgggtgact cctttttctt ttccgggcag ggacggtcat gcgataacgt catccatcat
     1081 cactgccctt accgtcacgc cgtctgataa catcatgccg ttatcatcat caccatgata
     1141 acaagcatcc atataaccac gccgttaaat taccggagag ccgtttccgt cagatccgac
     1201 tttccggaat atcacaactt tccggaagtg taccacgctg aggaaatcat catatcgctg
     1261 ttttccgccc ctgcgcgacg catcacaggt cagggagatg acacaacata tagtgtcatc
     1321 agcagctgaa tccggcacca catgttgtga tgacgggagg tggcgttgtg tggggggaga
     1381 ggaagatcct gaacagccgg aaaccggatc tgcgcggcgg gaaacgggca g
//
LOCUS       DAZUCC010000452         1383 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZUCC010000452 DAZUCC010000000
VERSION     DAZUCC010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.455"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1124)
                     /locus_tag="ACHY09_005251"
     CDS             complement(<1..1124)
                     /locus_tag="ACHY09_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3888054.1"
                     /translation="MKINRHGITLSLISFLIAGAPAFAQDGSSWASPDTASASETDST
                     AVGAWANAGKAGTALGSASYAHAPFSTSVGMSAYVNPEASNGVAVGSWASADALNSVA
                     LGTGAQAMHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTR
                     DTDAATVGQLNRKADEVYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNT
                     RGLLSQRDVLETHEERLNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQ
                     EMATENRAAVSRNRQVGERNSRAIASQARTLQAHERRMNSQQRQINENHEEMKRAAAQ
                     SAALAGLFQPYSVGKFNATAAVGGYSDKQAVAVGMGYRFN"
BASE COUNT          351 a          405 c          298 g          329 t
ORIGIN      
        1 tcgttaaagc gatatcccat gcccaccgca acagcctgct tgtcactgta cccgcccaca
       61 gccgctgtgg cgttaaattt acccacactg tatggctgga aaagaccggc cagcgctgct
      121 gactgggctg ctgcccgctt catttcctca tgattctcat taatctgacg ctgctgactg
      181 ttcatacgac gctcatgcgc ctgcagggta cgtgcctgag aagcaatggc gcgactgttg
      241 cgttcaccca cctgacggtt acggcttacc gcagcacggt tttctgtggc catttcctgt
      301 gtacgcacca gcccttcctg tacagtaatt cgttcaccgg ttccctcacc gcgcgtcaca
      361 taaccatgac tgtccacagc aacggtactt ccggtattta tctgctgttg ctggctgttc
      421 aggcgctctt catgcgtctc cagcacgtct ctctgggaaa gcagccccct ggtgttacgg
      481 tttatggcca ccgtgttccg gataatattc tccctgttct ctgcagcaac cgtatcggta
      541 tggtcacgcg ccttaagtgc ttccgctgca atccgtccac tgacgtcact gtacacctca
      601 tccgccttcc tgttcagttg cccgacagtg gctgcatccg tatcccgtgt accatcactg
      661 acaccaccga gaacccgggt tttatccggt acacgcggac tggccgggtt tgtgacacct
      721 gagtcatatc cgatgtagac ttcattttca cgggttgtac cggactgctg accaagagcc
      781 actgaatacc tgtgcattgc ctgtgcaccc gtacccagtg ctactgaatt taaagcatct
      841 gcagatgccc agctacctac tgccacacca ttagacgcct ccggatttac ataagcagac
      901 atccccacgg atgtggaaaa cggcgcatgc gcatacgatg cagagccaag agctgtccct
      961 gccttcccgg cattcgccca tgcccccact gccgtggagt cagtctctga tgcagaggct
     1021 gtgtccggag acgcccagga actgccatcc tgcgcaaacg ccggagcacc ggcaatcaga
     1081 aaggaaataa gcgataaagt aataccgtgt cgatttattt tcataaacaa cctcattgat
     1141 atacacaata tactggcaca gagttaaatt aatatataat caggagagaa gtgaaagagt
     1201 acccggtgcg aaaatgaatt aatctgagca gatttaacgg taaacgaagc gcaataaatt
     1261 aaaattaaaa aacaaaatca cttgcagtct attatcttta cgttcctcac cgcaaccgca
     1321 cacaaaaaca aaacacacaa tgcattgatt ttaaacaaaa aaacaaaaca gaaactcaac
     1381 aca
//
LOCUS       DAZUCC010000453         1381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZUCC010000453 DAZUCC010000000
VERSION     DAZUCC010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.456"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            133..948
                     /gene="cma"
                     /locus_tag="ACHY09_005252"
     CDS             133..948
                     /gene="cma"
                     /locus_tag="ACHY09_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin colicin M"
                     /protein_id="HFS3888055.1"
                     /translation="METLTVHAPSPSTNLPSYGNGAFSLSAPHVPGAGPLLVQVVYSF
                     FQSPNMCLQALTQLEDYIKKHGASNPLTLQIISTNIGYFCNADRNLVLHPGISVYDAY
                     HFAKPAPSQYDYRSMNMKQMSGNVTTPIVALAHYLWGNGAERSVNIANIGLKISPMKI
                     NQIKDIIKSGVVGTFPVSTKFTHATGDYNVITGAYLGNITLKTEGTLTISANGSWTYN
                     GVVRSYDDKYDFNASTHRGIIGESLTRLGAMFSGKEYQILLPGEIHIKESGKR"
     gene            complement(998..1351)
                     /gene="cmi"
                     /locus_tag="ACHY09_005253"
     CDS             complement(998..1351)
                     /gene="cmi"
                     /locus_tag="ACHY09_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFS3888056.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWPEDKGLACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEEYVEYAND"
BASE COUNT          386 a          309 c          260 g          426 t
ORIGIN      
        1 ctgccctacc atttttaacg gcatatcagg caaacaatgt tatggggagc ctctctcatg
       61 gggtttgttc gccggcggag gcgttagcca gcggcgaact ctgttatctt gttaacttat
      121 aaggagttat gtatggaaac cttaactgtt catgcaccat caccatcaac taacttacca
      181 agttatggca atggtgcatt ttctctttca gcaccacatg tgcctggtgc tggccctctt
      241 ttagtccagg ttgtttatag ttttttccag agtccaaaca tgtgtcttca ggctttaact
      301 caacttgagg attacatcaa aaaacatggg gccagcaacc ctctcacatt gcagattata
      361 tcgacaaata ttggttactt ctgtaacgcc gaccgaaatc tggttcttca ccctggaata
      421 agcgtttatg acgcttacca cttcgcaaaa ccagcgccaa gtcaatatga ctatcgctca
      481 atgaatatga aacaaatgag cggtaatgtc actacaccaa ttgtggcgct tgctcactat
      541 ttatggggta atggcgctga aaggagcgtt aatatcgcca acattggtct taaaatttcc
      601 cctatgaaaa ttaatcagat aaaagacatt ataaaatctg gtgtagtagg tacattccct
      661 gtttctacaa agttcacaca tgccactggt gattataatg ttattaccgg tgcatatctt
      721 ggtaatatca cactgaaaac agaaggtact ttaactatct ctgccaatgg ctcctggact
      781 tacaatggcg ttgttcgttc atatgatgat aaatacgatt ttaacgccag cactcaccgt
      841 ggcattatcg gagagtcgct cacaaggctc ggggcgatgt tttctggtaa agagtaccag
      901 atactgcttc ctggtgaaat tcacattaaa gaaagtggta agcgataata taagggccgg
      961 ggttccggcc tttttatgca gcctttatag cagtgtatta gtcattcgca tattcaacat
     1021 attcctcctc acaatggtac atgccataat acattctctt accctccggg ccttcagctt
     1081 taaatgggac aagaagtgtt ttttcatctt caacatctgt taaacttctc tcaatctttt
     1141 cccatgtaat tttcgggatt tctgtaccaa gaagttgtac atcgttgtcc cagcgtttca
     1201 ttctttgcag gtaatcattt tttacaaacg ttctggcctg ttcatcgctg acctgatagc
     1261 atgccagacc tttatcttca ggccagaaaa aaacagcagc aagagcaata atcgttaaaa
     1321 taaaaataaa tttcatgcta attactttca taaaatctcc acaacttctg tcgttcattc
     1381 g
//
LOCUS       DAZUCC010000454         1369 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZUCC010000454 DAZUCC010000000
VERSION     DAZUCC010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.457"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /locus_tag="ACHY09_005254"
     CDS             <1..231
                     /locus_tag="ACHY09_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFS3888057.1"
                     /translation="KAGKKDGYWPPDPVEENTGTRNHKWQRLTPSVSTSCAVFPDGEH
                     EAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            245..>1369
                     /locus_tag="ACHY09_005255"
     CDS             245..>1369
                     /locus_tag="ACHY09_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFS3888058.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGVEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAM"
BASE COUNT          338 a          302 c          463 g          266 t
ORIGIN      
        1 aaagcaggga agaaggacgg ttactggcca ccggatccgg tggaggaaaa tacgggcacc
       61 cggaaccata aatggcagag actgacgcct tcagtctcca cgtcctgcgc agtcttccct
      121 gacggagagc atgaagcggc tgaaacaggt aatgctgcct ttgccctgtg gcagccctac
      181 agttgctgca agaaacgggg acagacattc ctgggcagta cgaattttta agtaagggga
      241 agtgatgaaa cagaaaatga cgatacctgt tctggctgtg gtgctttgtg cgggtacggt
      301 acaggctgac ggaaactttt tgtccctgcc gaaagtgaat gacagtgcgt ttggctacgg
      361 taaatccgtg gacggcgcag tatcagacaa actgttttat accctgggag ggggctcggt
      421 gatatcgcag ccggcaacgc gcagcaatat gcagaaactt ggggtggatc tcgggtggag
      481 cagtgatctg atgtgtggca attttgacct gaaaacaaca gtgggtaacc agctgaacgg
      541 gatcactgat ggtttcaaaa acctgatggg ggatgtgatt cagggggcga gcggagccgt
      601 ggcgagtctg ccggcaatgg tgatacagcg ggctaatccg gggctgtatg acatgctgac
      661 caacggtgta ctgcaggcga atgtggcatt cgacaaagcg cagctcaatt gtcagaacat
      721 ggcgaaacgc atgatggagt tcacggacag caataagtgg acgcaggcgg ccatgctcga
      781 ggagtacaaa aaaattgtga atacggggga cggtgatgcg atcaaagggg atgaagcagg
      841 tcgtcaggtt accggggtgg aggggcaacg ctggatcgga gggcagcagc gtggtggagc
      901 gggacagtct gctatccggc cgacacatga tctgacagct gccggtttca atatgatgaa
      961 cagtctgcct tcgaccagta catccagtgt cagtcccggg aactgtaacg ggagtgcctg
     1021 catgaaatac cgtaatgcag aggaagctgc ccgggcggtg gtgaaggttc tgggggataa
     1081 atccatgcgg acctgtggtg atgcaaaggc atgtaccagc ggggatgctg atcagcagcc
     1141 ggggaccagt attgccggca cggggtttgc gccgatgctt gaagaagcga cgcgggttaa
     1201 cacggaactg ctggcaggta tggtcaatgg tcgtgacaag ccgacagccg ggaatctggc
     1261 aaaactgaaa gccgggagtc tgtctgtgac agcgggagtg atccgtgccc tgcagcgtga
     1321 tccggacaat ggtgccctga cgtcgcgtct ggcgggagaa ctggccatg
//
LOCUS       DAZUCC010000455         1360 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZUCC010000455 DAZUCC010000000
VERSION     DAZUCC010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.458"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1360)
                     /locus_tag="ACHY09_005256"
     CDS             complement(<1..>1360)
                     /locus_tag="ACHY09_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFS3888059.1"
                     /translation="ILCKHLIVNTEFDWLAFPAGYNSLFIPPKSPPASNLKPEPLPVV
                     NLPLSSPAVNPVYAKSCLKEKGCTDAGTAEEPAENFGQVAIFAQPVVDDCCGYHHPEA
                     NDVGQPAEAQTMLLFPGSVLAAQIWGKWSLSGILSATRGSYIGALASALYIPSAGEGS
                     ARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGKPQVYGVHTGEGTPYENVR
                     VANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPGHTGNDRPPLDQPTILVTP
                     IPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVYLKEDLRKLPGVVTGRGVP
                     LSLGTRWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEFREALWLEVSKDSVLLAQF
                     IKSNQNNVSQGYSPYVPEEGYYYGPNEIVKKFQIHHVVAIEHGGGVYDIDNFRIVTPR
                     LHD"
BASE COUNT          336 a          361 c          329 g          334 t
ORIGIN      
        1 atcatgtagt cggggcgtaa caatcctaaa attatcaata tcatacactc caccaccatg
       61 ttctatagca actacatgat gtatctggaa tttcttaact atctcatttg gtccgtaata
      121 atacccctcc tcagggacat agggggaata tccttgacta acgttattct gattactttt
      181 tataaactgc gccaataaca ctgagtcttt actcacctcc agccacagcg cctcgcgaaa
      241 ctcatcaaag gttttaaact cccgtccgcg caatttatca gcaatatgcg ccgggattgg
      301 tgcgccgttg ccgttattgg ataccgacat atccagccag cgagttccta gtgaaagcgg
      361 gacgccgcgc cctgttacaa caccaggcag ctttcgcaga tcctccttca ggtaaacata
      421 gatgggctta ataccggatc ccgccggaaa aaccagaata taatcgttga attctttcgg
      481 atcaggaacc gggaatggcg gcgtggtata ggtatcggtg ccgtccggaa tcggcgtcac
      541 cagaatggtg ggctgatcca gcggcggcct gtcgttaccg gtatggcccg gaacattccc
      601 atgttccgga ttttccggcg tccaggtaat cagggggcca tcgacatcgt gagcgggggt
      661 aaattcataa cgctgcgtct gctcgttcca ctgcatgttc gccacgcgga cgttttcata
      721 cggcgtacct tcacccgtat gaacaccata cacctgaggc ttgccgtgaa tgtcagttcc
      781 ccagaaaaaa cgtacccggg tggtggcggt actgcctgct agtgctttct gccgcagttc
      841 ttcctcatac cagaactcat cacgtccggg cacgcgagca ctgccctcgc ccgcagaggg
      901 aatatacaaa gcagatgcca acgcaccgat gtaagagccg cgggttgcac tgagtatgcc
      961 actgagcgac cattttcccc atatttgagc cgccaacaca ctacccggaa acagtagcat
     1021 ggtttgagct tctgcgggtt gcccgacatc gttagcctcg ggatggtggt atccacagca
     1081 gtcatcaacc actggctgag caaaaatcgc tacttgaccg aagttttcag cgggttcttc
     1141 tgccgttcca gcatctgtac atcccttctc cttcagacag gattttgcat agaccgggtt
     1201 aaccgctggt gaagagagtg gaaggttaac aactggtaga ggttcaggct taagattact
     1261 ggcaggtggg cttttaggcg gaataaataa gctattatag ccagcaggaa aggccaacca
     1321 gtcaaattcc gtattgacta ttagatgttt acataatatg
//
LOCUS       DAZUCC010000456         1356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZUCC010000456 DAZUCC010000000
VERSION     DAZUCC010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.459"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..348)
                     /locus_tag="ACHY09_005257"
     CDS             complement(<1..348)
                     /locus_tag="ACHY09_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFS3888060.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRVAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTKKNPAKIKEAALLHISGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYA"
     gene            complement(569..1300)
                     /gene="bluR"
                     /locus_tag="ACHY09_005258"
     CDS             complement(569..1300)
                     /gene="bluR"
                     /locus_tag="ACHY09_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFS3888061.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          349 a          314 c          304 g          389 t
ORIGIN      
        1 ggcgtagtaa ctgcgtttgt tatgattaac atcagtaacc caccggaaaa cgcccgcgtc
       61 tgccagtgtt gaacagtact cccgaaatgt agattttccg gaaatatgaa gcaatgcggc
      121 ctcttttatt ttagcagggt tctttttcgt actaactttt aacaggttcc tggttcctct
      181 taataacaaa accgtgtcat cgtgagtaat aattctgatg ttatccgtag ccagataata
      241 aatgtaatgt gcaacgcggt gatgttttaa ttctgaataa aaccaggaga agttttgctc
      301 ttttctcact tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac
      361 ctcttgttag ttgtgagact gcgtagtgtg cacgatcggt tttaccactt caatctggtc
      421 tgtcctttgg ctgtgatatg tacaaagtgt gaaaggggga ttacctgaat tctcccggtg
      481 agcattttgc aacggaccag ctccggtaca aaagctgttg tgggttcaga ttataagatt
      541 ctgtctaagg ggcgggataa aggtgaaatt agggggcatg aaagatgact ttataacctt
      601 gctcacccca gtgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga
      661 caatcaatgt ttgcgtcggg aacagttccg ggcgcggcga ttcaatggga tcagcaagaa
      721 cagaaatgtg ccatcctgac aatgataaac gccatgcttc cagccacaat cgtgctctct
      781 cttcaacatt ccacgccatc agaatggctt ctttaccggg cttacggcgc atttcgaaaa
      841 gtgaggctgc tgcgtattca attaatgcgc cgtcaaacat actgctcata atgcgggagg
      901 tgttgtgatc aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat
      961 ctactgggta ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca ggattagcca
     1021 tacgaagaat tgacatcatc tcttcttgca ggcgactcca gtcatcttcc gtatcctggc
     1081 tggtggtttc cagtaatgct ttaactttgc ctacaggaac gccattactt atccaacgct
     1141 tgatctcttc gatgcgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc
     1201 gctgtggttt taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc
     1261 cgcaacgttc agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcaca
     1321 aatctccgtc gcctgtacac gacccaataa tacttt
//
LOCUS       DAZUCC010000457         1345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZUCC010000457 DAZUCC010000000
VERSION     DAZUCC010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.460"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            329..592
                     /gene="ykgM"
                     /locus_tag="ACHY09_005259"
     CDS             329..592
                     /gene="ykgM"
                     /locus_tag="ACHY09_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS3888062.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            592..732
                     /gene="ykgO"
                     /locus_tag="ACHY09_005260"
     CDS             592..732
                     /gene="ykgO"
                     /locus_tag="ACHY09_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFS3888063.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(767..992)
                     /gene="ykgL"
                     /locus_tag="ACHY09_005261"
                     /pseudo
     CDS             complement(767..992)
                     /gene="ykgL"
                     /locus_tag="ACHY09_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            1139..>1345
                     /locus_tag="ACHY09_005262"
     CDS             1139..>1345
                     /locus_tag="ACHY09_005262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888064.1"
                     /translation="MQCPGKEAIVPVTTGSLRLTSLNLALGGLTGQMTYLHLFNFSSL
                     IGMNTHNHFVRHVNPLKHLTDWQII"
BASE COUNT          383 a          300 c          280 g          382 t
ORIGIN      
        1 aatatgcatc ctctcccttt tttgtaagta attattatat ccgtgggaga ggaatacaca
       61 ttgtcaggta atcaatcatg ctgcaataaa tcatcggcca gtaaagtgga gatagcctcc
      121 attctcgaaa aatccatact ctcagcgaaa ccatcatcaa tcactcatcc aggcgtttat
      181 gggagcgtcg ccaatggctg ctaacaatgc cagacttccc cgttgcggaa attccacatc
      241 ccacaaatag tcacagtgat tgggtgttga aatgatccgg atgagcatgt atctttacgg
      301 ttatgttata acataacagg taaaaatgat gaaacccaat atccatcctg agtatcgtac
      361 tgtggtgttc cacgacacca gcgttgatga gtactttaaa atcggctcga ctatcaaaac
      421 agaccgtgag attgagctgg atggcgtaac gtatccgtac gtaacaattg atgtctcttc
      481 gaaatcgcac ccgttctata cagggaagct gagaacagtg gcatcagaag gaaatgttgc
      541 acggttcacc caacgttttg gtcgttttgt tagcacgaaa aagggggcat gatgaaagtc
      601 cttaactctc tgcgtaccgc aaaagaacgc catccagact gtcagattgt gaagcgaaaa
      661 ggacggctgt atgtgatttg taaatctaat ccacgtttta aggccgttca gggtcgtaag
      721 aaaaaacgtt gattcaaaat tcgacgaatt aacgatattt gtctgattaa taatcatacc
      781 ggattaatgt tggtgtattt attacaccag cattaatttt cctggggatg tatcttcctg
      841 ttaatttaag gtcaactgcc tgacgtttct ctccgaatat tccattatat taatgttgac
      901 ttgttgacca gctgcgccct gtatgctggc atcaactcta ttttagactg aacacgccac
      961 tcagtttcct ccctttgcga cgcagcctgc atttacactc aaactgttaa gatgataaat
     1021 gtggtaaatc tgttggtact gacataaaaa cgtttacgcc acaggaacgg ccagatccat
     1081 cggtaacccc atcgccgacg ttcgagtgcc agttagagta acgcgtacag atgactgaat
     1141 gcagtgccct ggcaaagagg ccatcgttcc tgtgacaact ggcagtcttc gtttaacttc
     1201 acttaatttg gctcttgggg ggcttaccgg acagatgacg tacttacacc tgtttaattt
     1261 ttcatcactt attgggatga acacccacaa ccattttgtg cggcatgtta atccattaaa
     1321 acaccttact gattggcaaa tcatc
//
LOCUS       DAZUCC010000458         1342 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZUCC010000458 DAZUCC010000000
VERSION     DAZUCC010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.461"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1241
                     /locus_tag="ACHY09_005263"
     CDS             <1..1241
                     /locus_tag="ACHY09_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3888065.1"
                     /translation="LVCFITVINYLDRTALGIAAPTIMETTGITKEQYSWIVSAFQLA
                     YTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLAFMRALMGFSEASA
                     IPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMFHSWQFAFIVSGSL
                     ALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKTSIKHILSQRNFWG
                     IGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLGDLGCLASGFVAKF
                     FHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIGAFSHQCLSTVAAT
                     LGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPFFIALAFFDIIGAI
                     ALWMLIKVKDEEPQVQLATS"
BASE COUNT          322 a          297 c          319 g          404 t
ORIGIN      
        1 ccctcgtatg ctttatcact gtaatcaatt atctggacag aacggcgtta ggtattgcgg
       61 ctccaacgat tatggagaca accggaataa ccaaagaaca atattcatgg attgtcagtg
      121 cattccagtt ggcctataca ttggggcaac cgataatggg cttctttatt gataccgtgg
      181 gtctgaagtt aagttttgcg atatgtgccg caatctgggg cctggcgaca atggggcatg
      241 ccttgacagg aacatggtca ggtctggcat ttatgcgcgc cctgatgggt ttcagcgaag
      301 catcggcaat tcctgcgggt gtaaaaaccg catcaacatg gttcccggca aaagagcgtg
      361 gcgtggcgac aggtgttttc aatatgggca cctcactcgg cgcgatgctt gctccaccgt
      421 tgattgcctg gtgcattatg tttcatagct ggcaatttgc gtttattgtc tcaggtagcc
      481 ttgctttact cgcggcttta ttttggttct tttgttataa agatccaaaa gatgctaaac
      541 gcctttctga tgaagagcgc cactatattg agtctgggca agaacagcat cttaaaacag
      601 ataagaaaga aaaaacgtca atcaagcata tcctcagcca acgtaatttc tgggggattg
      661 gcatcgcgcg ttttctcgct gacccggcat ggggaaccat taacttctgg gtaccgattt
      721 tcttcgtcga aacgctgcat tttagcctga aagaaattgc catgttcgtc tggctacctt
      781 tcctgctggg cgatctcggc tgtttagcca gtggttttgt cgcgaagttc ttccacgatc
      841 gcggcgtgag tttaattaac tcacgaagaa ttaccttcac tattgcagcc gtcattatga
      901 tgacgattgg tctggtgagt attgtcgaaa atccctacat cgccgtatta ctgattagca
      961 ttggcgcatt ctcgcatcaa tgtctttcta ctgttgcagc aactctgggc ggtgacctat
     1021 tcaaaaaaga cgaagtagct accgctgtgg gtatggcagg agcctgtgca tggagcggtc
     1081 agttgatttt caacctgttt atcggggcat tcgttcacat tattggcttc gcgccgttct
     1141 ttattgccct ggctttcttt gacattattg gcgccattgc gctgtggatg cttatcaaag
     1201 ttaaagacga agaaccacaa gtacagttag cgacaagcta attattttac atagaaacgc
     1261 tataatttag ctttgtagcg tttctatttt tctcataaat taaacgtctc ttccaaaacg
     1321 ttcagtatct actggcattg ct
//
LOCUS       DAZUCC010000459         1332 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZUCC010000459 DAZUCC010000000
VERSION     DAZUCC010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.462"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            58..1269
                     /gene="bluF"
                     /locus_tag="ACHY09_005264"
     CDS             58..1269
                     /gene="bluF"
                     /locus_tag="ACHY09_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFS3888066.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELMCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
BASE COUNT          376 a          285 c          290 g          381 t
ORIGIN      
        1 cggaagatat gtctgttacc gtcttactct cgcctctccc attaccctgg acagttaatg
       61 cttaccaccc ttatttatcg tagccatata cgtgacgacg aacctgtcaa aaaaatcgaa
      121 gaaatggttt cgatagcaaa tcgcaggaac atgcagtctg acgtaacagg gatcttactg
      181 tttaatggtt ctcatttttt ccagcttctg gaaggtccgg aagaacaggt taaaatgata
      241 tatcgggcta tatgccagga tccacggcac tataatattg ttgagctgat gtgcgattac
      301 gcgcctgctc gccgttttgg caaagcggga atggaattat ttgatttgcg cctgcacgag
      361 cgagatgacg ttttacaggc cgtattcgac aaaggcacat caaaatttca gctaacttat
      421 gatgacagag cgctacaatt ttttcgtact tttgtccttg caaccgaaca agcaacctat
      481 ttcgagatcc ctgccgaaga ctcctggctt tttatcgctg acggatctga taaagaactt
      541 gattcctgta ccctttcacc aaatataaac gaccactttg cctttcatcc tattgtcgat
      601 cccttatcgc ggcggataat cgcttttgaa gccattgtgc aaaaaaatga agacagcccg
      661 tcagctatag cggttgggca gcgtaaagaa ggggaaatct atacagcgga tctcaaaagt
      721 aaggcgcttg cattcgcgat ggcacacgca cttgacctcg gtgataaaat gatttcaatc
      781 aatctattac ctatgaccct ggttaacgaa cctgacgcag tctctttttt acttgatgaa
      841 ataaaggcca atgctctggt gcctgaacaa atcatcgttg aatttactga aagtgaagtc
      901 atatctcggt ttgatgagtt tgccgaagcg gttaaatcgc taaaggctgc tggtatcagt
      961 gtagcaattg atcattttgg cgcaggtttt gccggtttgt tactcctgtc acgcttccag
     1021 cctgacagaa ttaaaatcag tcaggaattg attaccaatg tccataaaag tgggccacgg
     1081 caggcaatta ttcaggcgat cataaaatgc tgtacatcac ttgaaattca agtctctgct
     1141 atgggtgtga caacaccaga agagtggatg tggcttgaat ctgcaggaat tgagatgttt
     1201 cagggagatc tgtttgcgaa agctaaattg aatggtatcc cttcagttgc gtggccagag
     1261 aaaaaataat tttcagcaca ttatttcaca tgatttcaca tgatttccgt aaatcatcgt
     1321 gaaaatgaat ac
//
LOCUS       DAZUCC010000460         1323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZUCC010000460 DAZUCC010000000
VERSION     DAZUCC010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.463"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            833..>1323
                     /locus_tag="ACHY09_005265"
     CDS             833..>1323
                     /locus_tag="ACHY09_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HFS3888067.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRT"
BASE COUNT          356 a          271 c          335 g          361 t
ORIGIN      
        1 aacctgaaag gggatgtgca gctaggatgt ttctggtggt ggtaaatctg cccacctttg
       61 agagcagata tcgtcagtca tgatgatgaa ggtttctttt aaccgcgccc tttattacgg
      121 gcaatacaca gcagtggttg tgtgatcctt atctatccgg tgatgccgga atgttcttac
      181 cgcgtaatgg cggtattgac ttcttttatt cttttcattt gtttgccagc acttcctgcg
      241 actgtgcgcg ctgtataaga ccatttgaaa tttggtcgct tacgcgtgta tctggcgtct
      301 gaagctgcgc ggcgtcactt tcgtcagcgt gtcacgatga caaagtgacg cacgcgcgtc
      361 tgcttaacga acccgcacga actgccgttt cacgtgatgt tttttaacat ttgaagaagt
      421 gacccggcgt gagagtgttt tttcaacctc ttctgcaaat ttacggacga ttttgtgaat
      481 aacgtccata aatggttttt tgcagaatca attgtttttt aaggagtttt atgtagtgag
      541 atgtcgaatc ggcgatacca cttattagag gtggtgtcgg gggcgacctg gagacaggta
      601 cagcaaatgc tgcagtcggt gtgcaacacc gatttgttac ccctcaatac ctaaacaatc
      661 tgcgctaccc tggcgcacag gcagtcacta cccgaaggat cgcaacgttt aaccgtgtcg
      721 tcagggagaa atgcagatga gtttttctgt tgtgttcacg gcacaacaga aaaactcatc
      781 tgccgaacgt acagctgttt aaggtaaaaa ttattatgtg caggagaagt tgatgtccag
      841 acgtgaaaaa ttactgaaac aggatctggt tgctcacatt cgaaaagaca aaggcagctt
      901 caagaagctg agtgattaca ccaggatcat gcaccgcttt gctgaaagtc tggcaaaaat
      961 gaatgtacag atcagttctg ccgcgcaggt aaaagtccgg catattgagc tatatatgag
     1021 aagccggagt aatgtttctg ggcgaacaag ggaaaatgaa atgtctgcaa tacgggttat
     1081 gttacgtcag gcaggtaaat ataagatggc tgatcccgaa catcctcgtc tcagcaataa
     1141 agcgttaggc atttccggct ccagtcgtaa gggaacaaaa aaattaatca cggatgagcg
     1201 ttttcgggag attcttcagc gtgtggaagc aaaagataaa ggggtggctg ctgttgttaa
     1261 actgtcacgt tacctcggtc tgagaaatga agaagctatc cagtcggcca aatcattacg
     1321 aac
//
LOCUS       DAZUCC010000461         1320 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZUCC010000461 DAZUCC010000000
VERSION     DAZUCC010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.464"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..1285
                     /locus_tag="ACHY09_005266"
     CDS             38..1285
                     /locus_tag="ACHY09_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFS3888068.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
BASE COUNT          376 a          262 c          391 g          291 t
ORIGIN      
        1 gagcagatat gtttaacagt ttgtggaccg cagggatatg acagaaaaaa cggaacggaa
       61 taaagcgtcg tcagaggtgg tcaggcagtc agacctggcg ctgttgacag atgtacaggc
      121 agcccttcag caggaaaagc atcatgccct tttcctgatg gtgatttttc tgctggtact
      181 ggtagtgacg tttgttatct gggcatggaa cagtcctctg gatgaagtga cccgaggtca
      241 ggggagtatt attcccggca gcagggagca ggttatacag acccttgacc cgggtatact
      301 gaaatcactt gaggtcaggg aaggcgatat tgttgagaaa ggtcaggtac tgctgacact
      361 ggatgatacc cggagctctg ccatgctgag ggaaagtgaa gcccgggtga ataacctgga
      421 ggccgtcagg gcaagactcc gtgcagaagc ttattccgaa tcactgacat ttccggataa
      481 cgttcctgag gatttgcggg agcgggaaag tacggtctat tttctccgga agacggagct
      541 ggcgcagagc attgccggtc tgaagcagag taaggcgctg ctggacaggg aaatagcaat
      601 gacgcggcca attgtgcgtg aaggggcaat gtcggaggtc gaactgttac gtatgcagcg
      661 tcagtcagcg gagctgcagt tacagatgga tgagaaacag aataaatacc tgacggaagc
      721 cggtgctgaa ctggtgaaga cagaagcaga gcttgctcag gcaaaagaga atatggctgg
      781 cagagccgac ccggttgagc gttcacgtct cagggccccc ctgagaggta tcgtaaaaaa
      841 tatacgggta aacacactgg gaggggtggt ttctgcagga caggacataa tggaaattat
      901 tccgctggaa gaccagttgc tgattgaagc ttatataaat ccacgggatg ttgcctatgt
      961 cagaacaggg atgccggcac ttgtaaaact gacggcttat gactatgcca tttatggtgg
     1021 tcttgacggc gtggtgacac tggtaagtcc ggatacttta cgtgaccaga aacggccggg
     1081 agatttaaaa ctggacccga atgaagctta ttaccgggta ctggttacca ccagtaataa
     1141 ttatctgaca gacaggaacg gaaaaagatt gccagttatt cctggcatga tagccagcgt
     1201 ggatataaaa accgggcgta aaacggtatt tcagtatctg attaaaccga taaccagaat
     1261 gaagcaggca cttcaggaaa gataataaaa aggtcttccg gggaaagtat tttaaatcaa
//
LOCUS       DAZUCC010000462         1309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZUCC010000462 DAZUCC010000000
VERSION     DAZUCC010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.465"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1309
                     /locus_tag="ACHY09_005267"
     CDS             <1..>1309
                     /locus_tag="ACHY09_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFS3888069.1"
                     /translation="VNMIHDNDCVILGGFIGAVVPECIEKGIEDRFLKTGKPRNLKII
                     FAAGQGDAGTKAVNHLSHEGLVTSAIGGHWGLIPKLQKLANEGKITGYNLPQGVICHL
                     FRDSAAGKLGTLTHVGLGTFVDPRVEGGKINDKTQEDIVSVTEINGVENLFYKKIDAN
                     IAILRGTTSDQSGNITMEDECLILENLAAAQLVHNNGGKVIVQVKQIVRDGTLDPLQV
                     RIPGIYVDAVVVSEDEYHMQTFAEYMNFSYVGKGVKEVKDKETRPLDIKKIIARRAAM
                     ELKKNAVVNYGIGAPEVIAEVTDEEGITSELIATVEPGAIGGTPAGGLSFGASSFPQA
                     IITQDQMFDFYDGGGLDQAFLGLAETDEKGDLNVSKFGTKIAGCGGFINITQNAKKVY
                     FCGSFTAGKNKIYVSNGKLHIKEDGSVKKFIKKVQQITFSSDTA"
BASE COUNT          427 a          191 c          314 g          377 t
ORIGIN      
        1 gtaaatatga ttcatgataa tgattgtgtt attcttggcg gatttattgg tgcagttgtt
       61 cctgagtgta tagaaaaagg catagaagat cgttttctga aaacgggaaa accaagaaat
      121 ctcaagatta tttttgctgc tggtcaaggg gatgcaggaa caaaagctgt caatcattta
      181 tctcatgagg ggcttgtcac tagtgccatt ggcgggcatt ggggactaat acctaaattg
      241 cagaagttag ctaatgaggg aaaaattacg ggttataatc ttcctcaagg ggttatttgc
      301 catttattcc gagatagtgc tgctgggaag ttagggacat taactcatgt tggtttaggt
      361 acatttgttg atccccgcgt agagggcggg aaaattaatg ataaaacaca agaggatatt
      421 gtgtccgtca cagaaattaa tggtgtggaa aacctatttt ataagaagat tgatgctaac
      481 atagcaatcc tcagaggtac tacatctgat cagagtggga atataacaat ggaggatgaa
      541 tgtttgattc ttgaaaacct ggctgctgcc cagttagtcc ataataatgg agggaaagtt
      601 attgttcagg taaaacaaat agtacgtgac ggtacattag atcctttaca agtcagaatt
      661 ccaggtattt atgttgatgc tgttgttgtt tctgaagatg aatatcatat gcaaactttt
      721 gcggagtata tgaacttcag ctatgtcggg aaaggtgtaa aagaggtaaa agacaaagaa
      781 acacgccctc ttgatataaa aaaaattata gctcgacgag ccgcaatgga actgaaaaaa
      841 aatgcagttg taaattatgg cataggggca cctgaagtta tagctgaagt aacagatgaa
      901 gaggggatta cctccgaact tatcgcaact gttgaaccag gtgcaattgg aggaacgcct
      961 gctggaggtc tgtcatttgg agcttcttcg ttccctcagg cgataattac tcaggatcag
     1021 atgtttgatt tctatgacgg tggtgggctt gatcaagcgt ttcttggatt agctgaaaca
     1081 gatgaaaaag gagatttaaa tgtttctaaa tttggaacaa aaattgcagg gtgcggagga
     1141 tttattaaca taactcaaaa tgctaaaaaa gtatatttct gcggttcttt cactgctgga
     1201 aaaaataaga tttatgtcag caatgggaaa ttgcatataa aagaggatgg gagtgtaaaa
     1261 aaattcatta agaaggttca acaaatcact ttctcgtcag atactgcaa
//
LOCUS       DAZUCC010000463         1249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZUCC010000463 DAZUCC010000000
VERSION     DAZUCC010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.466"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            301..494
                     /locus_tag="ACHY09_005268"
                     /pseudo
     CDS             301..494
                     /locus_tag="ACHY09_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            536..865
                     /locus_tag="ACHY09_005269"
     CDS             536..865
                     /locus_tag="ACHY09_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3888070.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            846..1127
                     /locus_tag="ACHY09_005270"
     CDS             846..1127
                     /locus_tag="ACHY09_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3888071.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
BASE COUNT          354 a          310 c          303 g          282 t
ORIGIN      
        1 cactttagtt acaacactca gatacaacca ccagaaaaac ccaagtcccg cgcagaactg
       61 aaaccacaaa gcccctcctc ataactgaaa agcggccccg ccccggccca aagggccgga
      121 acagagtcgc ttttaattat gaatgctgta actaatcatc ttcatcgctg tcagtcttct
      181 ggctggaagt cctcagtaca cgctcgtaag cggccctgac ggcccgctaa cgcggagata
      241 cgccccgact tcgggtaaac cctcgtcggg accactccga ccgcgcacat aagctctatc
      301 atagctgaca gcgggtatgg ctttgcaggg tggggatggg taaggtgaaa tctatcaatc
      361 agtaccggct tacgccgggc ttcggcggtt ttactccggt atcacatgta accacagcgt
      421 gccgccttac atgcgctgac gcgcataaac aacaagttgc tacattagct tacaggttaa
      481 ttcaccttgc ataagattag catataggtt aatgtttatc gaaatgggag gtcatatgtt
      541 tagcgttgtt tatcacccgg aagccaggga agaagccaca gcattaccca taaaaatcag
      601 ggtgaagttt gaccgtctca tcagcaaact ggaatatgac gcccgattac tgcgggaacc
      661 ggatacaaag cccctggggg atggactttt tgagatccgc acgatgggga cggacatagc
      721 cagaggtatc tgggtatacc acaaaggcaa taccatcatc atgctccggg ttttcatcaa
      781 gaagtctcag aaaacgccag caaaagaaat cgatctcgcc aaaaagcggt tagcggaggt
      841 acttaatgaa actggtaagc cataaagaac tgcataacga gtggatgcag gacgacgaat
      901 atcgcgctgc atggcaggaa gaagaacgta aggagaaact cagggagttg cttgcagaat
      961 ggcgtaagca tgacaatttg acaaaagcgc aggttgctga acgcatgggg gttactcctc
     1021 ctgttatttc ccgcctggaa aacaacatca ctaaagccag catcgacacc ctgactcgct
     1081 atgcccatgc gtgcggtatc aaaaaacctg ttattacgct gtactgaact ctgctctatg
     1141 gctgccagtt ctggagaaat cagtaactaa attttaatta gctacgttat ggtaaatctg
     1201 cctgatgtgt cgatttctct gaaagtaatt cggcttctgt gggctgcgt
//
LOCUS       DAZUCC010000464         1235 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZUCC010000464 DAZUCC010000000
VERSION     DAZUCC010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.467"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..859
                     /locus_tag="ACHY09_005271"
     CDS             101..859
                     /locus_tag="ACHY09_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFS3888072.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(912..1019)
                     /gene="yniD"
                     /locus_tag="ACHY09_005272"
     CDS             complement(912..1019)
                     /gene="yniD"
                     /locus_tag="ACHY09_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFS3888073.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT          311 a          279 c          295 g          350 t
ORIGIN      
        1 tttataattc gttacctttc cagggacgct aatctttgat ccttaagaaa gtttccggga
       61 ccgcgaaggt ctcttttttt gttgtacgga ctcttgataa atgaagcttt tgaagacagt
      121 tcctgccatc gttatgttgg cggggggcat gtttgcgtca ctgaatgcag ctgccgatga
      181 ttccgttttt actgtcatgg acgatcctgc cagcgctaaa aaacctttcg aaggtaatct
      241 gaacgcagga tatcttgcac aatcaggcaa tacgaaaagc tcctcactta cggctgacac
      301 caccatgacc tggtatggac aaaccactgc ctggtcgctg tgggggaacg ccagtaatac
      361 ttcttctaat gatgagcgtt cttcagaaaa atatgcggcg ggcgggcgta gccgttttaa
      421 tttaactgat tatgactatt tatttgggca ggcaagctgg ctgacagacc gttataacgg
      481 ctatcgagag cgcgatgtgt tgaccgcggg ttatggtcgc cagtttttga atggcccggt
      541 acacagtttc cgctttgaat tcggtccagg tgtgcgttac gacaaatata ccgataatgc
      601 cagtgaaacc cagccgctgg gttatgcctc cggggcttat gcatggcagt tgactgacaa
      661 cgctaaattt acgcagggtg tttccgtctt tggcgcggaa gacacaacgc tgaactctga
      721 aagcgcatta aatgttgcta ttaatgaaca ttttggactg aaggtggctt acaacgtaac
      781 atggaactct gaacctccag agtctgcacc ggaacatacc gatcgtcgga caaccttatc
      841 gctgggctac agcatgtaaa attatctgcc ggaccgtgtt atgcggtctg gctgtcttcg
      901 tttgtcactg ctcagcccca aatcattgct gcccaacgta gcaacaacat agcgccacag
      961 attgccagta gcaccaccac catcttccag tgttttttat caaagcgttt agtcggcata
     1021 ccagtgtcct gcatatctct tgtgcgtgat aacggagtgt aacaaaaggg acaggcatca
     1081 agggattttt tgttattaca gccgtcgggt ggcgttgtac acctgatcac tgtttttgcg
     1141 gtttatgaaa aaaataaaat tatgtatcga tatataaata tctggttata aatacgtctt
     1201 tagttcatca gcactaaaac tcaggctttg gctga
//
LOCUS       DAZUCC010000465         1231 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZUCC010000465 DAZUCC010000000
VERSION     DAZUCC010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.468"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1231
                     /locus_tag="ACHY09_005273"
     CDS             <1..>1231
                     /locus_tag="ACHY09_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFS3888074.1"
                     /translation="GFDGRTQRYHYDLTGKLTQSEDEGLVILWYYDESDRITHRTVNG
                     EPAEQWQYDGHGWLREISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHE
                     TKQAYNEQGLTNRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLLEYMRDRLHRETVRSF
                     GSMAGNTAAYELTSTYTPAGQLQSRHLNSLVFDCDYNWNDNGDLMRISGPRQTREYGY
                     SATGRLASVRTTTSDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAEDAHYVY
                     RHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYD
                     PLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDHLTTVQTDTTRIQTVYEPGSF
                     TPLIRIETDNGEREKTQR"
BASE COUNT          290 a          363 c          407 g          171 t
ORIGIN      
        1 gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa ctcacgcaga
       61 gtgaagatga ggggcttgtc atcctctggt actacgatga atcggaccgt atcactcacc
      121 gcacggtgaa cggcgaaccg gcagagcagt ggcagtatga tggccacggc tggctgagag
      181 aaatcagcca cctgagcgaa ggccaccgtg ttgccgtcca ctatggctat gacgataagg
      241 gccgtctgac cggggagcgc cagacggtgg agaacccgga gacgggggaa ctgctgtggc
      301 agcatgagac gaaacaggca tacaacgaac aggggctgac aaaccgcgtc acgccggaca
      361 gcctgccgcc ggtggagtgg ctgacctatg gcagcggtta tctggcgggc atgaagctgg
      421 gcgggacgcc gctgctggag tacatgcggg acagactgca ccgggagacg gtgcgcagct
      481 tcggcagcat ggcaggcaat acagccgcat atgaactgac cagcacttac acccccgcag
      541 gccagttaca gagccggcac ctgaacagcc tggtgtttga ttgtgactat aactggaatg
      601 acaacggcga cctgatgcgc atcagcggcc cgcgacagac gcgggaatac ggctacagtg
      661 ccacgggcag gctggcgagc gtgcgcacga ccacatcaga cctggatatc cgcatcccgt
      721 atgccaccga cccggcggga aaccgcctgc cggacccgga gctgcacccg gacagtacac
      781 tcacagtgtg gccggataac cgcatcgcgg aggatgcgca ctatgtctac cgccacgatg
      841 aatacggcag gctgacggag aagacggacc gcatcccggc gggtgtgata cggacggacg
      901 acgagcggac ccaccactac cactacgaca gccagcaccg cctggtgttc cacacgcgga
      961 tacagcatgg cgagccactg gtcgagagcc gctacctcta cgacccgctg ggacggcgaa
     1021 tggcgaaacg ggtatggcgg cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta
     1081 aaccggaggt gacgtggtac ggctgggacg gcgaccacct gacgacggtg cagaccgaca
     1141 ccacgcgtat ccagacggta tacgagccgg gaagcttcac accgctcatc cgcatcgaaa
     1201 cagacaacgg cgagcgggag aaaacgcagc g
//
LOCUS       DAZUCC010000466         1221 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZUCC010000466 DAZUCC010000000
VERSION     DAZUCC010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.469"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1052
                     /locus_tag="ACHY09_005274"
     CDS             <1..1052
                     /locus_tag="ACHY09_005274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888075.1"
                     /translation="DRIGTVIYTGNVNMTQGHGITTGSGQKVKIADDDLQKDSKSFLA
                     IKAHEANVGTNVYVISKNGANNVALLEMTEGDNIITTELSLGNLGVSRWVRPMVVDTG
                     AGDDTIVTGRDLGIGYGDLWTEVNTGIGDDILIVGFGRGGYDVVYGKDGSYRIEETAK
                     YQRLDGDIIIPDNDSGYPNNGGFIEKTTVTMGDGNDSLLLLGGNSLAALKSSTLDLGA
                     GNDVIDIVANIDNASNIKGGSGFDTLVIHQGNATSNTFSGFEKIELKDGAALNLLAEN
                     LNSASIEGNKLKVTGDGSSRVDLGNNGDNLSDNGYNWIKGSTESEGDITYNIYTHASV
                     PDVQVLIDERITNVV"
BASE COUNT          359 a          181 c          309 g          372 t
ORIGIN      
        1 tagatcggat tggaaccgtt atctatacag gcaatgtaaa catgacgcaa ggtcatggaa
       61 ttacaactgg ctcaggtcaa aaggtcaaaa tcgctgatga tgatttacaa aaagattcta
      121 aatcattcct ggccattaag gctcatgagg caaatgtagg gacaaatgtt tatgtaataa
      181 gtaagaatgg tgctaataac gtcgctctac tggaaatgac agagggggat aatataatta
      241 cgactgaact gtcactgggg aatctgggcg tttctcgctg ggtaagacca atggttgtcg
      301 atactggtgc aggtgacgat accatcgtga ctggtcgtga tttggggatt ggatatggtg
      361 atctatggac cgaggtaaat acaggtatcg gtgatgatat cctgatagtt ggttttggtc
      421 gaggcggtta tgatgtcgta tatgggaagg atggttctta ccgcattgaa gaaaccgcta
      481 agtatcaacg actggatgga gatatcataa tacctgataa tgattcggga tacccaaata
      541 atggtggttt tatagagaaa acgactgtta ctatgggaga tggaaatgac agcctgttat
      601 tgttaggagg taattctctt gctgcgctca aatcatcaac cttggattta ggtgctggta
      661 atgatgtaat cgatattgtg gctaatattg ataatgcttc aaatattaaa ggtggttctg
      721 gatttgatac cttggttatt catcagggta atgccacaag caacacattc tctggttttg
      781 agaagattga actgaaagat ggagctgcac tgaatttgtt ggctgagaat ttaaattctg
      841 cctctataga gggtaataaa ctcaaagtca caggtgatgg tagttctcgg gttgacctcg
      901 gtaataacgg ggataacctc agtgacaatg ggtataactg gattaaagga agcactgagt
      961 ctgaagggga tatcacttat aacatttata cacatgcttc ggttcctgat gtacaggttc
     1021 tgattgatga gagaatcact aatgtagtgt aacaatagta tcccctcaca gtcgttgctg
     1081 tggggggatc tttttcgttg taagttctac tccaggtatg ctcttttact gaaaaaattt
     1141 atctgagaat aatgcttttt gtttcttctt ggataaatga aggatattta aatgaattca
     1201 gaggagattt tatgggggtg t
//
LOCUS       DAZUCC010000467         1215 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZUCC010000467 DAZUCC010000000
VERSION     DAZUCC010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.470"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..734
                     /gene="ais"
                     /locus_tag="ACHY09_005275"
     CDS             132..734
                     /gene="ais"
                     /locus_tag="ACHY09_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFS3888076.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(773..1198)
                     /gene="nudI"
                     /locus_tag="ACHY09_005276"
     CDS             complement(773..1198)
                     /gene="nudI"
                     /locus_tag="ACHY09_005276"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFS3888077.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
BASE COUNT          318 a          279 c          265 g          353 t
ORIGIN      
        1 tggcaaaatt ttgaggcatt ttgccggcat cgtaggattt ttaatattac accaactgct
       61 aattatcgcc agaaatgtcg ctcaatttca cggtaattgt ctggttgcgc ttgtctatag
      121 gtggagttta cgtgttagct ttttgccgct cttcgttgaa gtcaaaaaaa tatttcatca
      181 ttttactggc gctcgctgca attgccggac tgggtactca tgccgcctgg agtagcaatg
      241 gtttgccacg tatcgacaat aaaacgctgg ccagactggc acagcagcat ccggttgtcg
      301 ttttgtttcg ccatgctgaa cgttgcgatc gttcaaccaa tcaatgcttg tcagataaaa
      361 caggtattac ggtcaaaggt acccaggatg cccgtgaact gggcaacgct tttagtgctg
      421 atatccctga tttcgatctc tattccagta ataccgtccg gaccattcag tcggcgacct
      481 ggttttcagc gggtaaaaag ctgacggtag ataaacgact tcttcaatgc ggtaatgaga
      541 tttatagtgc aattaaagac ttacaacgca aagcgcctga taaaaatatc gttattttta
      601 cccataatca ttgcctgaca tatattgcta aagataagcg tgatgcgaca tttaaacctg
      661 attatctgga tggtttagtc atgcatgtgg aaaaaggcaa agtttatctg gatggggaat
      721 tcgttaacca ctaaattttc aatctgacag ccagtgatgg ctgtcatcat tattacagaa
      781 gacctttcaa acgtaacgtt tttcgggtgg cgacattcaa atcatagtgc gccagatctt
      841 caggttttac ccacgcgtag tcttgaaact cttcgtttat tttcacttct cggttggcag
      901 aaacgcagtc aaaaatcagg taaatcatat aaatctcttc cttgcgacca tctgcatacg
      961 tcttggtgcg aatatcatcg ctgaaggtcc acggcgtgat ttctgtcaaa agcagctgtt
     1021 ctcccagctc ttcgcgaatt tcgcggcgta gtgcctcttc aattcgttcg ccgggctcca
     1081 cgccgccacc cgaaagcgcc cattgaccgg ggaaaacgcc gcgatcgtcg gccattttac
     1141 acagcaaata agcaccatca ttttgaatca aagggcatac aatagtccgt tgtcgcacag
     1201 gggtattcct tataa
//
LOCUS       DAZUCC010000468         1214 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZUCC010000468 DAZUCC010000000
VERSION     DAZUCC010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.471"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..390
                     /locus_tag="ACHY09_005277"
     CDS             <1..390
                     /locus_tag="ACHY09_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFS3888078.1"
                     /translation="MKNLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSE
                     KQLTVTSSEDEIIISTPETLTLNGGGSYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGV
                     DLPNEAPGFSLTDITQESKISSKSFND"
     gene            406..>1214
                     /locus_tag="ACHY09_005278"
     CDS             406..>1214
                     /locus_tag="ACHY09_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888079.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPVPFKGEQAIRCMADGEVIAYRICRDYLT
                     IAWESGPLNFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYESEAENQWVVQD
                     TLKAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWNPADSNLNATGSNKRKYGLVTL
                     KGLPEKNSSTLTPGQRYWVVVDNNNVKPAPGAGPGWWRHLLPPAKEAMVFD"
BASE COUNT          329 a          272 c          334 g          279 t
ORIGIN      
        1 atgaaaaatc tgtcggtaac ggtggaaaaa gcccttggtc tgtttgtgca taaagacggg
       61 gcgaagctgg tagccaacca gggagatatt gagattcagg ctcagcacaa cacgatggcg
      121 ctgttctctg aaaaacagct gacggtgacc agtagtgaag acgaaattat tatcagcacc
      181 ccggaaactc tgacgctgaa cggtggtggg tcttacctga gactgagtaa gaacgggata
      241 gagcacggct catcgggtga gtttattgtg aagacgtctg actatctggt gcctggttct
      301 ggcgtagatc tccccaacga agcacctggt ttcagcctga cggatatcac acaggaaagc
      361 aaaatcagca gcaagtcttt taacgactga cagggagtat gaatcgtgtc tgtattaccc
      421 aaaatcgtct ggccgatccc ctccagccgt cggggaagtg agttcaaaaa tcaggaagag
      481 atcctctctc atttgggggg agaatctacc gggctgtaca tgataggccg tagtggcatg
      541 tggcatggtg ggatccatat caccgaagcc acgacaccat ggtgtgcgct tagcggtaag
      601 tctccacttg aagcgataga ttttccggtt ccttttaaag gtgagcaggc aatccgatgc
      661 atggctgacg gagaagtgat tgcttaccgc atctgcaggg attatctgac gattgcatgg
      721 gaatccggtc cgctgaattt ttccggctca tttgttctgg tgaaacacta tatccagccg
      781 ggtgagaaag aaaccagtgg actgcatttc tatactctgt atatgcatct tgcgccgtat
      841 tcagcttatg aatctgaagc agaaaatcag tgggtcgttc aggacactct aaaagcatat
      901 tctgaaatgg actggctgac ggcgaaactg acttcagatg aaacttctcc acaaattgca
      961 gggtacatgc ctgaaggggc cagagtggag tggaacccgg cagacagcaa tcttaacgct
     1021 acaggaagta acaagagaaa atacggtctt gtgacgctaa agggtttacc agaaaaaaac
     1081 agcagtactc tcactcccgg tcagcgctac tgggttgttg tggataacaa caatgttaag
     1141 ccagcgccgg gggccgggcc cggctggtgg cgacacttgc tgcctcccgc aaaggaagcc
     1201 atggtattcg ataa
//
LOCUS       DAZUCC010000469         1212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZUCC010000469 DAZUCC010000000
VERSION     DAZUCC010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.472"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHY09_005279"
     CDS             complement(<1..1001)
                     /locus_tag="ACHY09_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFS3888080.1"
                     /translation="MRSFLTSKADSIVQQLSGLSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRIS
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPSRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYCVSGHSSSPYLTPGQVLEADG
                     "
     gene            complement(986..>1212)
                     /locus_tag="ACHY09_005280"
     CDS             complement(986..>1212)
                     /locus_tag="ACHY09_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888081.1"
                     /translation="YDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHMAAKQKP
                     QALTLAWSDEDGIVLVFDAQAEGESSCAVS"
BASE COUNT          274 a          364 c          315 g          259 t
ORIGIN      
        1 ccgtccgctt caagtacctg acccggcgtc agataaggac tggaggaatg cccgctgaca
       61 caatactgcg cgttcagaat gcgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgc cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgtccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgctc aagacggctg
      421 tccatttcat aacgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagctcc cgggatggat actcccggga gagccggaac
      541 tcaaaatcct gcccttccag accatgttta cgcaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcaat acggggaacg
      661 atcgtcagtg cataacgggt ctcatcattc gaggcagaaa tgcgacgaaa cgactcaacc
      721 acgccgaata ccgttctgag cggcagggcc ggtgttccgt cagacaccgg ggtctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga agagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgatagccc tgaaagctgc
      961 tgtacaatgc tgtcagcctt gctggtcagg aaactgcgca tgacgactct ccctctgcct
     1021 gtgcatcaaa taccagaaca atgccatcct cgtcactcca tgccagagtc agcgcctgcg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagttgct
     1141 ggttcagcag actctccaca ttccgggcgc ccgtatccgg cagcaggcag gcggatgtca
     1201 gggcatcgta ca
//
LOCUS       DAZUCC010000470         1212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZUCC010000470 DAZUCC010000000
VERSION     DAZUCC010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.473"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1001)
                     /locus_tag="ACHY09_005281"
     CDS             complement(<1..1001)
                     /locus_tag="ACHY09_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFS3888082.1"
                     /translation="MNRSLTSKAGSVVQQLSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQVPMSDGTPALPIRTVFGVVESFRRIF
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTTGDAESPETGAYFARLRHERILNAQYCVSGHSSSPYLTPGQVLETDG
                     "
     gene            complement(998..>1212)
                     /locus_tag="ACHY09_005282"
     CDS             complement(998..>1212)
                     /locus_tag="ACHY09_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888083.1"
                     /translation="YDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHMAAKQKP
                     QALTLTWSDEDGIVMVFDAQAEGEPS"
BASE COUNT          270 a          360 c          313 g          269 t
ORIGIN      
        1 ccgtccgttt caagtacctg acccggcgtc agataagggc tggaggaatg cccactgaca
       61 caatactgcg cgttcagaat gcgttcgtga cgcagacggg caaagtatgc cccggtctcc
      121 gggctttcag cgtcacctgt cgtcaggaag ggttcagcat aatggtacgt ttctccggtg
      181 gtgatccctt ccttactgaa aatactttcg gcactgtcct gaggtttcag cgcttcacgg
      241 tagttgtagt cccgggtgac cacacttccg ctcaccacgt tacaggtggt ctgaatatcc
      301 cagatgctct cctgcccgct gtcactcatc ccggcctgat tgcgcaatgg cagcgtgatg
      361 ccagattcat actgctgctg gctgtcctgg aaaatcacta tgtcctgttc aagacggctg
      421 tccatttcat agcgccagta aatccccacc tccgccagca ggcgccggat aaactcaagg
      481 tcagtttccc gccaccgggt gatgagttcc ctaggtggat actcccggga gagccggaat
      541 tcaaaatcct gcccttccag accatgttta cgtaatacct gctcaaccac ttcagtgact
      601 gactgattga ggtagatttc gctccctttc gtatagttca gaagggcgat acggggaaca
      661 atcgtcagtg cgtaacgggt ctcatcattc gaggcaaaga tacgacgaaa cgactcaacc
      721 acgccgaata ccgtcctgat cggcagggcc ggtgttccgt cagacatcgg gacctggaaa
      781 gtaaatgacg ccggcttcag cagtaacgcg tcacaggcaa tatccgcaga agagcaggtg
      841 acaaccacgt catagcgcca gggctggctg agggactcaa ccgcgctgta acgcaacaca
      901 tccagaaaat ggctgcattc atgcacatga acccggtacc gtgattgccc tgaaagctgc
      961 tgtacaacgc tgccagcctt gctggtcagg gaacggttca tgacggctct ccctctgcct
     1021 gtgcatcaaa taccatcaca ataccgtcct cgtcactcca tgtcagagtc agcgcctgtg
     1081 gtttctgctt cgcggccata tggctcagca actgccggct cagtaccggc agcagttgct
     1141 ggttcagcag actctccaca ttccgggcgc ccgtatccgg cagcaggcag gctgatgtca
     1201 gggcatcgta ca
//
LOCUS       DAZUCC010000471         1210 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZUCC010000471 DAZUCC010000000
VERSION     DAZUCC010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.474"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..274
                     /locus_tag="ACHY09_005283"
     CDS             <1..274
                     /locus_tag="ACHY09_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFS3888084.1"
                     /translation="NNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAW
                     LAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNPLPVPR"
     gene            274..549
                     /locus_tag="ACHY09_005284"
     CDS             274..549
                     /locus_tag="ACHY09_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3888085.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPVAARMVQSLIQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(815..1210)
                     /locus_tag="ACHY09_005285"
                     /pseudo
     CDS             complement(815..1210)
                     /locus_tag="ACHY09_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858194.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          278 a          334 c          290 g          308 t
ORIGIN      
        1 aaacaatacc gcacaggcaa caaaagtaat taccgcgcat gtgccattac ctatggctga
       61 taaagtcgac cagatggccg ccagactgga acgctcccgg ggctgggtta tcaaacaggc
      121 actttctgca tggcttgccc aggaggagga gcgtaatcgc ctgacgctgg aagccctgga
      181 cgatgtgata tccggacaga ttatcgacca tcaggctgta caggcctggg cagacagcct
      241 cagtactgac aatccgttac cggttccacg ctgatggaac tgaagtggac cagtaaggcg
      301 ctttctgatt tggcgcggtt atatgatttt ctggtgctgg ccagtaaacc tgtcgccgcc
      361 agaatggtac agtccctgat acaggcaccg gtcattctgt taactcatcc acgtatggga
      421 gaacagttgt ttcagtttga acccagggag gtcagacgga tttttgctgg cgagtacgaa
      481 atccgttacg aacttaatgg ccagactatt tatgtattgc gcctgtggca cacacgagaa
      541 aacaggtaga ttgcgtgtgg ggacaactta acatagcctg cagatattaa ttcaggaaca
      601 ttattatcag gccagagatt ttgcattcca ttaaataatc ttgacgtata ggaaattgaa
      661 aaactgagcg atacccctgt ccgcgcctta tgccacgtga gcttttctgc ttttcccacc
      721 actccggtgt tttctctcta tgtatggtcg gtttttatac cttaatgggc cgttttttat
      781 acttttcccg gattgcaggc gcacctctcc tgcatcagcc agggatatac ctcagcaggc
      841 ttatatcccg cgcgtttttc ttcagccctg atacattcag ccccgcaatg gcccggcggt
      901 ataccatccg gcagccacac agcacgtatt cgaacgggtc acgactcagg aactgcgctt
      961 caccatttgt gcatagcaca ctttcgccac tgcttccggt ttatccatcc ccagagcacg
     1021 gtacacctgc ggcagcttct ctccacacac ccggttggca aggaacccga agtacctcac
     1081 catcttaaaa aacttctccg ggatgtgctg tttcagcctc gcgaccagct cacgctgtgt
     1141 cagcgtttcc gtcgccgttt ctcccgtttt gtggtccagg taacggaagc tcaggctcgc
     1201 ccctccgttg
//
LOCUS       DAZUCC010000472         1209 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZUCC010000472 DAZUCC010000000
VERSION     DAZUCC010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.475"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1209
                     /locus_tag="ACHY09_005286"
     CDS             <1..>1209
                     /locus_tag="ACHY09_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS3888086.1"
                     /translation="KDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQRSI
                     TAPDRIGEGKTWVYQRDTMVERGDNRGVHQYGPAEHFTHIISDKPSPKDKYVAYAINI
                     PDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKP
                     GEAILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQ
                     PEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIR
                     AKISKDIESGIPGYWQGQVLIPFTIDPVTKERKQIHPEDIHLTAKDFETSKDNISKDE
                     WENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIE
                     RSITPPLTIY"
BASE COUNT          440 a          243 c          219 g          307 t
ORIGIN      
        1 aaaagacaga gaaattatat caacttttca cgcagaaaaa gtcattaatg atctgttaaa
       61 aaacgattat aaaatttccc tttctccaga tacaacttac cgagagctga ataaagctgc
      121 acagcgtagc attacagcgc cagacaggat aggagaaggg aaaacatggg tttatcaacg
      181 agatacaatg gttgaaagag gtgataacag aggcgttcat cagtatggtc cagctgaaca
      241 tttcactcac attatatctg acaaaccatc cccaaaagat aagtatgttg catatgctat
      301 taacattcct gactatgagc tagcagccga tgtatataat attaacgtga cgtcaccttc
      361 cggacagcaa gaaacattta aaatactaat caatccagaa catctacggc aaacacttga
      421 gcgtaaatct cttactgctg ttcagaaatc acaatgtgaa atcatcaccc ccaaaaaacc
      481 tggcgaagcg attcttcatg cttttaatgc cacctaccag caaatcaggg aaaatatgtc
      541 tgagtttgca cgttcccatt atgggtatat acaaattcct ccagtgacaa ctttccgcgc
      601 cgacggacca gaaactcccg aagaagaaaa aggttactgg tttcacgctt atcaacccga
      661 agatctttgt accatccata atccaatggg agatttgcag gattttatcg cattggttaa
      721 agatgctaaa aaatttggta tcgatatcat tcctgattat acctttaact ttatgggaat
      781 tgggggtagt ggtaaaaatg acctagatta tccctctgct gatatacgag cgaagatcag
      841 taaagatata gaaagtggta tccctggcta ttggcaaggt caggttttga ttccatttac
      901 tatagatcca gtaacaaaag aacgtaaaca aatccatcca gaagatatac atctcactgc
      961 aaaagacttt gaaacaagta aagataacat ctctaaggat gaatgggaaa acctccatgc
     1021 attaaaagaa aagcgtttaa atggaatgcc taaaacaacc cccaaaagtg accaggttat
     1081 tatgttgcaa aatcaatacg ttcgtgaaat gcgaaaatat ggcgtacgag gtttacgtta
     1141 cgatgcagca aaacactcaa aacatgaaca aatagaaaga tcaataactc caccgcttac
     1201 aatttataa
//
LOCUS       DAZUCC010000473         1209 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZUCC010000473 DAZUCC010000000
VERSION     DAZUCC010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.476"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..193
                     /locus_tag="ACHY09_005287"
     CDS             <1..193
                     /locus_tag="ACHY09_005287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888087.1"
                     /translation="DVLTRLPEWPEERLHDLLPFSAYRFAELTDVTVIPPNAGKRVME
                     VVSSTPMNDTASEVKIPTQ"
     gene            complement(345..>1209)
                     /locus_tag="ACHY09_005288"
     CDS             complement(345..>1209)
                     /locus_tag="ACHY09_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS3888088.1"
                     /translation="QLRPLYDELKHYVLMPGKVHADDTPVNVLEPGQGKTRTGRLWVY
                     VRDDRNAGSTMPAAVWFSYSPDRKGIHPQQHLADYRGILQADAYAGYNALYESGQVTE
                     AACMAHARRKIHDVHVRHPTTVTGEALRRIGALYAIESEIRGSPAEERLAVRKARTVP
                     LMQSLYEWLQGQMSTLSRHSDTAKAFTYLLKQWDALNEYCRNGWVEIDNNLCENALRV
                     VALGRRNYMFFGSDGGGESAAVMYSLIGSCKLNGIEPETWLRHVISVINTWPANRVKE
                     LLPWNVTLSVN"
BASE COUNT          280 a          345 c          310 g          274 t
ORIGIN      
        1 ggatgtcctg acacgtctgc ctgaatggcc ggaagagcgc ttgcacgact tactaccgtt
       61 ctcagcctac cgattcgcag aattaactga cgttacggtc ataccaccta atgcagggaa
      121 acgagtcatg gaggtcgtca gctctactcc catgaatgac acagcctctg aagtaaaaat
      181 accgacgcag tgaaaccagc cttaagcttt ccaaaacaca attacaacgt aagcgcacag
      241 cctgaaccgt ctggtcagaa tctgacgaat tagacaaagt ggtgtccacc aaataagtag
      301 tgggaaccaa aggtaagcgc accgtgaagg acgtggggta aaaattagtt tacagataga
      361 gtgacgttcc agggcaacaa ctctttcacg cggttggctg gccaggtgtt gatgacactg
      421 atcacgtggc gtagccacgt ttccggctcg attccgttaa gtttgcagct accgatcagg
      481 ctgtacatca ctgccgcact ctcgcctcct ccatcagagc cgaagaacat gtagttacgc
      541 cgccccagtg caactacccg gagggcgttt tcacacagat tattgtcgat ctccacccag
      601 ccattgcggc agtattcgtt cagggcgtcc cattgcttca gcagataggt gaacgctttc
      661 gccgtatcag agtgacgcga cagcgtgctc atctgcccct ggagccactc atacaacgac
      721 tgcattagcg gtaccgttct ggcttttctg accgccagtc gctcttctgc cggactgccg
      781 cggatctcag actcgatagc gtacagtgcc ccgatacgac ggagtgcttc tcccgttact
      841 gtcgttggat ggcggacatg tacatcgtgg atcttgcgtc gggcatgtgc catacaagcc
      901 gcttcggtta cctgaccgct ttcgtaaaga gcattgtaac ccgcatatgc atcggcctgc
      961 aggatacctc tgtagtccgc cagatgttgc tgcgggtgga tacctttgcg gtcgggagag
     1021 tatgagaacc acaccgctgc cggcatggtc gaaccggcgt tgcgatcgtc acgaacatag
     1081 acccacagcc gtccggtacg ggttttaccc tgacccggct ccagtacatt taccggcgtg
     1141 tcatcggcat gcactttacc cggcatcagc acatagtgct tcagttcatc atacagcggg
     1201 cgaagctgc
//
LOCUS       DAZUCC010000474         1202 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZUCC010000474 DAZUCC010000000
VERSION     DAZUCC010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.477"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(6..1142)
                     /locus_tag="ACHY09_005289"
     CDS             complement(6..1142)
                     /locus_tag="ACHY09_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFS3888089.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
BASE COUNT          356 a          291 c          213 g          342 t
ORIGIN      
        1 acctgttaaa actgatacat cataccaaca gcaagtatat tatcggttgc aactccagcc
       61 tttttggtaa attcgctttc atcaataagg ttaattttgt aatcaataag aagtgacatg
      121 tttttgttca gaaagtagtt tgcgccaaca tcaatatact caacaagaga ctgatcgcca
      181 tagtttacta ttccaccttt aacatccatc cctttggact gaacgtaagc cagtgaagga
      241 cgcaggccga aatcaaacat atactgagcc acaacttcaa tattccgtgc tttatctgcg
      301 atgcctgcgc taccaaatgg agtcatattt cgggtttcag agtagtcagc tgccagatac
      361 aggtcattat cattatactt gaccccaaca cctgcaaact ccgcatgttt tcccccggca
      421 atccagcgag gcgtaataac ttcttttcct tctgaatctt tcgatgtcat gaaacgttta
      481 cggccttctt tttcctgcag actggttctg tcggcattag tgtaagcggc aacgattcca
      541 aaaccttcat agtcatagct tacggagaaa ccatatccat caccattagc ttcaagtaaa
      601 ttatcacggt catttttacc ctgatattga gcagaaaaag taagtccatc aacaagcccg
      661 aagaaatctg tattgcgcca ggtcagaact ccggttgttc tgccggtcat atagttatca
      721 gagtaagtcc agctgtcacc accaaagtcc ggaagtacgt cagtcagagc ccccacatca
      781 taggcaacac catagtttcg cccataatca agtgaaccga gtatattgtg gcgaatacct
      841 gcaaatgcca gacgattttt atcgttgcgt acttcacttt ctgtcttaaa ccccaggaac
      901 tggtattccc actgaccaaa gccagtcagg ctctcattaa tttgtgtttc gcctttgaaa
      961 ccaagacgtg caaatgttct gtcgccatcc atagaaccat cagagatgta atgacgggct
     1021 ttaacgcgac cataaaaatc cagtttatta ccgtctttat tatagatttc tgctgcctgt
     1081 gttgcctcac acagcattaa tgcagaaacg acagagcaca caaccatact tagttttcgc
     1141 ataaaaaaat cccctgtgca catttttagt atggttttaa aaattaaact atggcgaaaa
     1201 aa
//
LOCUS       DAZUCC010000475         1202 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZUCC010000475 DAZUCC010000000
VERSION     DAZUCC010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.478"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..842
                     /gene="yeeO"
                     /locus_tag="ACHY09_005290"
     CDS             <1..842
                     /gene="yeeO"
                     /locus_tag="ACHY09_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFS3888090.1"
                     /translation="SWPGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSY
                     FKPLNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALI
                     NLPGSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMAS
                     FYTQDPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRV
                     VVGYVLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPV
                     VSE"
     gene            943..1018
                     /locus_tag="ACHY09_005291"
     tRNA            943..1018
                     /locus_tag="ACHY09_005291"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:976..978,aa:Asn,seq:gtt)
BASE COUNT          250 a          251 c          330 g          371 t
ORIGIN      
        1 tctcctggcc gggactggga tttgtcgggg ctgggttggg tttaaccatt tctcgttata
       61 ttggcgcagt tgcaattttg tgggtgctgg cgattggttt taatcctgcg ctaaggattt
      121 cgttaaagag ctattttaaa ccgctgaatt ttagcattat ctgggaagtc atggggattg
      181 gtattccagc gagtgtcgaa tcagtgttat ttaccagtgg tcggttatta actcaaatgt
      241 tcgttgccgg gatggggacc agcgttattg ccggaaattt tatcgcgttc tcaattgcgg
      301 ctcttatcaa cttacccgga agtgcgctcg gttctgcctc tacgatcatt acaggccgaa
      361 ggttgggagt agggcagata gcgcaagcag agattcagtt gcggcatgtg ttctggctgt
      421 ccactcttgg attaacggcc atcgcctggc taacggctcc ctttgccggg gttatggcat
      481 cgttttacac ccaggatcca caggttaaac acgtcgttgt gattctgatt tggctaaatg
      541 ctttatttat gcctatttgg tccgcctcat gggtgctacc cgctggattt aagggggctc
      601 gcgatgcacg ttacgccatg tgggtttcta tgttgagcat gtggggttgc cgggttgtag
      661 tcggttatgt gctgggaatc atgcttggct ggggtgtggt tggtgtctgg atgggaatgt
      721 ttgccgactg ggctgtgcgg gccgtgctgt tttactggcg aatggttact ggacgttggc
      781 tgtggaaata ccctcgaccc gagccgcaaa agtgtgaaaa aaagccagtt gtgtcggaat
      841 aaacgacaaa atgcagatta tttcagcaaa cgatttcaaa tttaaaaaac aggctttgac
      901 attgtggatg ggcatcgcta atattcgcct cgttctcacg attcctctgt agttcagtcg
      961 gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa
     1021 ttctaaaaat tcgcttttta gcgcaatgtc actgacctta gttgagcatt gctttttaac
     1081 ggatagcggg tttttgacat cttaagcgcc ctcgaccttt atggttgagg gcgttttgct
     1141 atgaacgcca tcaccatttt cccctcgatt acaaaacttg agtaattcag tagtctcccc
     1201 tc
//
LOCUS       DAZUCC010000476         1174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZUCC010000476 DAZUCC010000000
VERSION     DAZUCC010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.479"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..630)
                     /locus_tag="ACHY09_005292"
     CDS             complement(<1..630)
                     /locus_tag="ACHY09_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS3888091.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVKIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTK"
     gene            complement(656..>1174)
                     /gene="rfaY"
                     /locus_tag="ACHY09_005293"
     CDS             complement(656..>1174)
                     /gene="rfaY"
                     /locus_tag="ACHY09_005293"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFS3888092.1"
                     /translation="FKVFAPKTKRNERFFKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
BASE COUNT          361 a          245 c          213 g          355 t
ORIGIN      
        1 tttcgtgtgc tgcgggtcac ttgccagcat agcaatggct ttattcataa aatcattttt
       61 cgcccatgct tcaagctgca gatatactac accggcgttg aaataattac ctggaagtcc
      121 ctctatagcc agacgcttcg ctggtttttc ctgcataaaa ggcacatctt taataacagc
      181 tgcgaactta tcgccatcaa aattaatatc aagcaatcca gctaatgagc ctttacagat
      241 gacatcagca tcgatatata acagattttc aataacttca ctcagatact ggaacgcaac
      301 taaacggaag aatgttgcat aagaccactg tttcaggctg gggaagatat caataaaggc
      361 actggagact ttataaattt ttacttcaac gttagcattt ttgctggtaa aagcgttaac
      421 acgttgtaga taatcttcat caatgtagtc ggtaaagaga tgaaacttaa ccgcaaggtc
      481 cgggttatgc ataacaactg attgcataga tactgccgca ccaaataaaa aattacggtc
      541 aataccgtaa gcgatattta acacacgatc ggtgttagca atttccccag agacagcgac
      601 ttcagtaata ctctcagcta ataaatccat gttggttccg ttttgactgt gtggtttacg
      661 ctttgccttt taatttttta ataaatttgc gtagtttggt tctgtagatt actgaatagt
      721 agccataatc ttttatttcg ttggcaatac ccaggtgacg ttccatggcc agacgatcac
      781 gagctttacg ctctgccgta cagcttttac cggataggtc aattatcctg acaccatctt
      841 tactaacgat aaagttaccc cggtgagggt cgcccgatag catatttaag gcatgcaatt
      901 tttccattga agccttaatt tctgctttaa cattttctgg aataatcggc atatcattga
      961 gttctacgcc ctcgacatac tcgataagca tgataaaaac actggcatag ttgaaaattt
     1021 tacgctcagc cagaaaataa aaatcattag gaaaagtaag acctgcactt ctgacacgat
     1081 cggtttcaac gatcaaattc tgatagtagt caccttttac aaaagattta aaaaaacgtt
     1141 cattcctttt cgttttggga gcaaagactt taaa
//
LOCUS       DAZUCC010000477         1172 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZUCC010000477 DAZUCC010000000
VERSION     DAZUCC010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.480"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(57..1001)
                     /gene="leuO"
                     /locus_tag="ACHY09_005294"
     CDS             complement(57..1001)
                     /gene="leuO"
                     /locus_tag="ACHY09_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFS3888093.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          350 a          265 c          246 g          311 t
ORIGIN      
        1 tcaataaaaa atgcagaata aaccagacat taatgtctga cctattctgc attcagttag
       61 cgtttgcaaa ttgagactaa ttgctcttcc atccactgat ggcctttatc gcgcccggca
      121 gcttcatgcc aggagagata acaggttctg ctgttttgtt ttaacggcag cggtaatacc
      181 tgtaactcta aggattcagc gaactcttca gccagccaac gcggcgcaat agcgaccaga
      241 tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata cgcgatactg
      301 gcttgcttat ctaccgtgtc ataccaggtt tgactaaatg acgcgaaacg atcgagcgaa
      361 accgcagcat gttgttcgtt ataaacatca tgtttcagta acgggccttt aattgttgga
      421 tgatttttgc tggctaccaa caccatttca tctttaaata atggcacact ggtaaattca
      481 gggcgatgga agtcttcata actaatcaca aactccgttt cctgataacg cagctgatgt
      541 tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc aatctgctca
      601 atgtgattat aaatctgcga ggtcagaatg ctgtctaacg ggctgcaaac acaaagatga
      661 aatacacgtt cactgctcgc gggttcaaaa cctgaaccag gcaattcatt ttgtactagt
      721 tgcaatgcct gacgaactga gccaaaaagt tgaaatgcgc gagcagtcgg ttgaatacca
      781 cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc aacagcgtta
      841 ctgaccgcag gttgcgacat tcccagaaca tgagcggcac gggtgatgtt ttgctcctgc
      901 atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg tggcttgctt
      961 aactccgccg tctctggatg atctgtttga acctctggca tacttaactc cactgtcaca
     1021 cttaactccc tttcccttat tggaatgcag agaaataatc ctcgagaata tgatttatca
     1081 tgcatataaa ataagaaaaa ggaaaatgag taaaattagg aaaacataaa gatacgggtt
     1141 ctgataaaaa cagaatccat tgccgtggaa ag
//
LOCUS       DAZUCC010000478         1169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZUCC010000478 DAZUCC010000000
VERSION     DAZUCC010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.481"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1169
                     /locus_tag="ACHY09_005295"
     CDS             <1..>1169
                     /locus_tag="ACHY09_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFS3888094.1"
                     /translation="NKLTLSGGNGAGQNGVVISGVASGLEGKELSTLTNTDKEMKNAA
                     SVADLKTAIDGVSTSMQGAGFALKDSDDKEVKQSLGQAIKVSGDSNITTTAITNGDKG
                     LKISLNNDLSVGSNKAPGSIKVNGTDGKEAVSLNGQDGTIKLTSAPAANGKSASATIA
                     VNAGKGGLTDAVGTTKTRLTYTPDGKSPAEELATMNDGLSFEGDDGKIIHKKLNEKLT
                     IKGGAGDNEDVTDSNIRVDANEKGDGLLVRMTRNLRDLASAVFGTENGPTTRIDNAGI
                     TITTPAAGGGQGNTVSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDL
                     KNALRDSSAALVNTGFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVR"
BASE COUNT          334 a          243 c          345 g          247 t
ORIGIN      
        1 taataagctg acattgtcag gcggtaatgg tgccggacaa aatggtgttg ttatcagtgg
       61 tgtggcaagt ggactggaag gtaaggaact ttcgacgtta acgaatactg ataaagagat
      121 gaaaaatgcc gccagtgttg cagacctgaa aacggcaatt gatggtgtgt ccaccagtat
      181 gcagggtgca ggtttcgcac tgaaagactc tgatgataag gaagtgaaac agtctcttgg
      241 gcaggcgatt aaagtatccg gggacagcaa tatcaccacc acagcaatca ccaatgggga
      301 taaggggtta aaaatatccc tgaataacga cctgagcgtg gggagcaata aggctcccgg
      361 cagcatcaaa gttaacggga ctgatggtaa ggaggctgtg agtctgaatg gtcaggatgg
      421 cacgattaaa ctgacatccg cgcctgctgc gaacggaaag tctgcgtcag caaccattgc
      481 tgttaatgca ggcaaaggtg gattaacaga tgctgtcggt accacgaaga cacgtctgac
      541 ttataccccg gatggaaaat caccggcaga agagctggcg accatgaatg acggtctttc
      601 ttttgagggt gatgacggaa agattattca taaaaaactg aatgaaaaac tgaccatcaa
      661 aggtggtgct ggtgataatg aggatgtaac agacagtaac attcgtgttg atgcaaatga
      721 aaagggagat ggtcttctcg tccggatgac cagaaacctc cgtgatttgg ccagcgcggt
      781 ttttggtaca gaaaacggcc cgacaacccg gattgacaat gcaggcatca caatcacaac
      841 acctgctgcg ggcggtggtc aggggaatac ggtttccctt accggctccg gcctgaataa
      901 cggtggaaat cagataacca gtgtggccag tggtctgaac ggcactgcgc ttgctgacgc
      961 cggtggagat gttctgaata atgcggccaa tatcggtgac ctgaaaaatg ctctccgtga
     1021 cagcagtgcg gcactggtga ataccggctt tggtctgcag gcggatgacg gaagcaaggt
     1081 gaacacgtct ctcgggaagc atatcacggt tggtggtgac gggcagaata tcagcacgac
     1141 tgttcagaac gggagcctga cggtcagac
//
LOCUS       DAZUCC010000479         1168 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZUCC010000479 DAZUCC010000000
VERSION     DAZUCC010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.482"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1168
                     /locus_tag="ACHY09_005296"
     CDS             <1..>1168
                     /locus_tag="ACHY09_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFS3888095.1"
                     /translation="HHLKIANSIRKLFLAIDIVYDNEKSVIGQQITAAKDDLRGKGKL
                     DAKDLRVIHPSEKNYIVKEGEINTQRFEFHMYSRVSNHLENGVFYAAESEQNKRLEDD
                     LIERSYWHKNKSILIEKTGLERLITPITQTLNELENTFSDKLARITIGINSDANEFVK
                     KQPHSNRLQWSLASKKWKTSIDNPIYNQIKNIGIVDVMKYVNNETNFLSVFNAISSRK
                     NNLEADYNDLLACIFGNGANYGVHRMASASDRSIGILRDVNDKFIRPETTGMANTVIS
                     DAIANLSVYKYWTINEDSPFGSVDGQKHTCRINTFKARYSAKYFRKGKGVSAMTLVSN
                     HVPLSTVVISPNEYEGHFAFDLLYNNTSEIQPKSLATDNHGINNVNFAILDIFG"
BASE COUNT          411 a          203 c          235 g          319 t
ORIGIN      
        1 gccatcacct taagatcgcc aattcaatcc gaaagctttt tctggctata gatatagtat
       61 atgacaacga gaagtctgtt attggccagc aaattactgc tgccaaggat gatctcagag
      121 gaaaaggaaa gctggatgct aaggatctga gggtaattca tccatccgag aaaaattaca
      181 ttgtaaaaga aggagaaatt aacactcaac gattcgaatt tcatatgtac agcagggtat
      241 ctaaccatct ggaaaacggt gtcttttatg ctgcggagag tgaacaaaac aagagactgg
      301 aagatgatct catagaacga agttactggc ataagaataa atcgattttg atagagaaaa
      361 caggccttga acgacttatc acaccaataa cgcagactct taatgagctg gagaatacct
      421 ttagcgataa actggctcgt ataaccattg gaataaactc agatgctaat gagtttgtga
      481 aaaaacaacc acactccaac agattacagt ggagtctggc cagtaaaaaa tggaagacca
      541 gcatcgataa tccgatttac aatcagataa aaaatatcgg aatagttgac gtaatgaagt
      601 atgtaaacaa tgagacgaat tttttgtctg tgtttaatgc catatcatcc aggaaaaata
      661 atcttgaagc tgactataat gaccttcttg cctgtatatt tggtaatggc gcaaattatg
      721 gtgttcacag aatggcatct gcatcagacc gtagtattgg aatactacgt gatgtgaatg
      781 acaaattcat tcgtccagaa acaacaggaa tggccaacac agtaatatct gatgcaattg
      841 caaatctttc tgtatataaa tactggacga taaatgaaga ttctccattt ggaagtgttg
      901 atgggcagaa gcacacttgc agaataaata catttaaagc ccgttattct gccaagtatt
      961 tcagaaaggg gaaaggggtt tctgcgatga cactggtatc taatcatgtg ccattatcaa
     1021 ctgtggtaat ttctccaaat gaatatgaag gtcattttgc atttgacttg ttatataaca
     1081 acaccagtga aatacaacca aagtcattag cgactgacaa tcatggtata aacaacgtta
     1141 attttgcgat tcttgacatt tttggcta
//
LOCUS       DAZUCC010000480         1167 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZUCC010000480 DAZUCC010000000
VERSION     DAZUCC010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.483"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..159)
                     /locus_tag="ACHY09_005297"
     CDS             complement(<1..159)
                     /locus_tag="ACHY09_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888096.1"
                     /translation="MAPDTTTGRDPPQRREARFAKRRCALHPGGLLPGCQERRPRRDR
                     GGATGTVAA"
     gene            459..986
                     /locus_tag="ACHY09_005298"
     CDS             459..986
                     /locus_tag="ACHY09_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS3888097.1"
                     /translation="MAVRGINKVILVGRLGKDPDVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPEPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            1044..>1167
                     /locus_tag="ACHY09_005299"
     CDS             1044..>1167
                     /locus_tag="ACHY09_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFS3888098.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDNGEHF"
BASE COUNT          283 a          323 c          356 g          205 t
ORIGIN      
        1 tgcagcgacc gttccggtcg cgccgcctct gtcgcgccgt ggcctgcgct cctggcatcc
       61 cggcaaaagg ccgccgggat gtaaggcgca acgccgtttc gcaaaccgcg cttcacgtcg
      121 ttgcggcggg tccctgcccg ttgttgtgtc cggtgccatt cacctgttta agcccctgtc
      181 gccgggggct gatacagcca caacctgccg cactgactgt ccagggtaaa tgaacagccc
      241 acaaaatttt ttacccaaaa cccggtaaaa aattttgcgt tctgcccagg acaggtgcgt
      301 caggccgtgg cagtgatgcc ccttgcggcg aaggttctca aacaggtgat ggtgaatacc
      361 ggagggaaca aagagcaggt ccccgcaaaa cgtcaaaacc gcggctcccg aaactcaaca
      421 gatgatgggg gctgaaaaca tcaacagaag gagacatcat ggcagttcgt ggcattaaca
      481 aggtcattct cgtgggacgt ctgggaaaag atccggatgt ccgttacatc cccaacgggg
      541 gcgcagtggc aaatctgcag gtggccacgt cagaaagctg gcgtgacaaa cagacggggg
      601 agatgcggga gcagacagaa tggcaccgcg tggtgctgtt cggcaagctc gcggaagtgg
      661 caggtgaata tctgcgtaag ggcgcgcagg tctacatcga ggggcaactc cgtacccgta
      721 gctgggaaga taacggtatt acccgttacg tcactgaaat ccttgtgaaa accacgggca
      781 ccatgcagat gctggggagt gcaccacagc agaacgctca ggcgcaaccg gagcctcagc
      841 agaatgggca gccacagagt gctgacgcga cgaaaaaagg tggcgcgaaa acgaaaggcc
      901 gtggacgtaa ggccgcgcag cccgagcctc agcctcagcc gccggagggg gaggattacg
      961 gattttcaga cgatatcccg ttctgactgg cctgactgtg acaaccgccc cgtcctgtgc
     1021 ggggcatcac cggagagatg aggatgagcg aatatttcag aatacttcag ggactgccgg
     1081 acggtccttt tacccgaaaa catgccgaag ccgttgccgc acagtaccgg aacgtcttta
     1141 tcgaggatga caacggagaa cattttc
//
LOCUS       DAZUCC010000481         1164 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZUCC010000481 DAZUCC010000000
VERSION     DAZUCC010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.484"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            18..167
                     /locus_tag="ACHY09_005300"
     CDS             18..167
                     /locus_tag="ACHY09_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888099.1"
                     /translation="MEWRLMISGKLITLAKTICVIVGVSFSLMMVALFLSIAWMALTS
                     AGLVG"
     gene            218..286
                     /locus_tag="ACHY09_005301"
                     /pseudo
     CDS             218..286
                     /locus_tag="ACHY09_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            288..1055
                     /locus_tag="ACHY09_005302"
     CDS             288..1055
                     /locus_tag="ACHY09_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFS3888100.1"
                     /translation="MNNNSAKPFLPWLGGKRRLAKHILPLFPEHTLYVEPFCGGAALF
                     FMKKPSCAEIINDINSDIVNLYRVVKYHQEEFSRQFQWMLASREMWNEVINQHTEYMT
                     DIQRAVRFFFIQKCAYGGRAYSRAFGVKTHRPQIIPGNTEQLLQEARDRLARATIEHL
                     DWEVCIARYDRPTTLFYCDPPYWLDGGYGIDFSFCNYERMAELARTIKGKMIISLNDT
                     QEMRRLFEGMNIHHVAHRYTVPRGKTSSVSELIICNF"
     gene            1082..>1164
                     /locus_tag="ACHY09_005303"
     CDS             1082..>1164
                     /locus_tag="ACHY09_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS3888101.1"
                     /translation="MLSAFTVILLVVCGALSLGLNHYRDNA"
BASE COUNT          289 a          243 c          312 g          320 t
ORIGIN      
        1 atcacaaaga cggattgatg gagtggcgtc taatgatatc aggcaaactc ataacgctgg
       61 cgaagaccat ctgtgtgatt gtcggcgttt cattttcgct aatgatggtt gctctttttc
      121 tttccatagc ctggatggcg ttgacttcgg cagggctggt ggggtgacag tgactgatga
      181 cgacaattta tttaaacaga tgagtactga atttattcgg gcaacggcat ttgccattcg
      241 cattgtggca attgcggttc taatccggga aatccgctgg tggtgaaatg aataataact
      301 ctgcaaaacc gtttctcccg tggttaggag ggaagcgtcg tctggcaaaa catattctgc
      361 cgttatttcc tgagcacact ctgtatgtgg aaccgttctg tggcggtgcg gcactgttct
      421 ttatgaaaaa accgagctgc gctgaaatca tcaacgatat aaacagtgat atcgttaatc
      481 tctaccgtgt ggtgaagtac caccaggagg aattttcccg ccagtttcag tggatgctcg
      541 ccagtcgtga gatgtggaat gaggtgataa atcagcacac ggagtacatg acggatatcc
      601 agcgggctgt ccgtttcttt tttatccaga agtgcgcgta tggcggaaga gcatacagtc
      661 gggcatttgg agttaaaact cacagaccgc agataatccc cgggaataca gagcagttat
      721 tgcaggaagc ccgtgatcgg ctggcgcggg caactattga acaccttgac tgggaggtct
      781 gtatcgcacg gtatgaccgc ccgacaacgc tgttttactg cgatcctccg tattggttgg
      841 atggtggtta cggcattgat ttttcattct gcaattatga gcgcatggca gaacttgccc
      901 gcacaataaa aggcaaaatg attatatccc tgaacgatac gcaggaaatg agacgattgt
      961 ttgaggggat gaatattcat catgttgctc atcgttatac tgtaccacgt ggtaagacaa
     1021 gcagtgtcag cgagctgata atctgcaatt tctgacaggg ggcatggtga gcataaaccg
     1081 aattctttcc gcgtttactg ttattctgct ggtggtctgt ggtgcgctta gtctggggct
     1141 gaatcattac cgcgataacg ccat
//
LOCUS       DAZUCC010000482         1164 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZUCC010000482 DAZUCC010000000
VERSION     DAZUCC010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.485"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..768
                     /gene="ompT"
                     /locus_tag="ACHY09_005304"
     CDS             <1..768
                     /gene="ompT"
                     /locus_tag="ACHY09_005304"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFS3888102.1"
                     /translation="DWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDW
                     MDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWFLNEPNYRLGLMAGYQESRYSFTAR
                     GGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSG
                     WVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRVTNKK
                     GDTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1000..>1164
                     /locus_tag="ACHY09_005305"
     CDS             1000..>1164
                     /locus_tag="ACHY09_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFS3888103.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGN"
BASE COUNT          370 a          242 c          241 g          311 t
ORIGIN      
        1 gactggaaat tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc
       61 cagatatcta tcggggctgc tggctggaca acactcggca gccgaggtgg caatatggtc
      121 gatcaggact ggatggattc cagtaacccc ggaacctgga cggatgaaag tagacaccct
      181 gatacacaac tcaattatgc caacgaattt gatctgaata tcaaaggctg gttcctcaac
      241 gaacccaatt accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca
      301 gccagaggtg gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc
      361 ttcccgaatg gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc
      421 ttgactggaa gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc
      481 tgggtggaag catctgataa cgatgaacac tatgacccag gaaaaagaat cacttatcgc
      541 agtaaagtca aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaacg
      601 cctaatgcaa aagtttatat tgaaggcgca tggaatcggg ttacgaataa aaaaggtgat
      661 acttcacttt atgatcacaa tgataacact tctgactaca gcaaaaatgg tgcaggcata
      721 gaaaactata acttcatcac tactgctggt cttaagtaca ccttttaaca acgttaaata
      781 gattttctcc ccggtggtat tgtatagccc cggggaattt gtttaccgtt aaaagcaaac
      841 aatatcccct aatcttgtaa gcatttaacc tgcgtcctgg cgtgtaacct cttaccgcaa
      901 cacaagactc aatatattca aattcgttat gccgtggcat catttaaccc cttgtaattc
      961 atcgctataa ttgatttaat tcacaaataa aactataaca tggtgaaact aatgaaaaaa
     1021 aacacagatg atggggctaa aatttacaca ccacttaccc taaagcttta tgactggtgg
     1081 gttttgggtg tatcaaatcg gcttgcatgg ggatgtccta caaaggaaca ccttcttcca
     1141 cactttctgg aacatttagg taac
//
LOCUS       DAZUCC010000483         1159 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZUCC010000483 DAZUCC010000000
VERSION     DAZUCC010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.486"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1159)
                     /locus_tag="ACHY09_005306"
     CDS             complement(<1..>1159)
                     /locus_tag="ACHY09_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888104.1"
                     /translation="FYYHIYNFLQKSYCPCLDYSGTESDEAAVKRYLREYLAELGFNR
                     EDFRENGKMYELGKYQGTIRKGYGKRKSLMKQYIKACKNEYKKDYREKKLDKSELDRI
                     LNNIDKFYYAFYSLSILLDMQRKVKILDSIAYYLRVLIREGLWVHGLYGYAARYLYDF
                     NIFDTTPYARALLERFHEFESGPKGALTRYIVSLDDKSQEYIESLKDMVFNLSDKKSY
                     DDVYLENIINYFEQLQNARGYVTRCYMLLAVFIYLIRRNKLHKALRFYDESQKYELPF
                     GYLPGAFSVLRIALEIKVNREKIKHGSLFELLDYVKAYQDAFMDLRVVTDPAYNEDEI
                     QYDANNFTLMRVIKMYNSMLANISTKSDIQPPYITGLLDNVERALDKINILI"
BASE COUNT          362 a          250 c          171 g          376 t
ORIGIN      
        1 tatcagaata tttatcttat ctaacgccct ctctacatta tcgagaagac ctgtaatata
       61 cggaggctga atatctgact ttgtactaat atttgcaagc atcgaattat acattttaat
      121 gacacgcatc agagtaaaat tattcgcatc atattgtatt tcatcttcat tatacgcagg
      181 atccgtcacg acccttaagt ccataaatgc atcctgataa gcttttacat aatcaagtaa
      241 ttcgaacagt gaaccatgct ttattttctc cctgtttact ttgatctcca gcgctatcct
      301 gagaactgaa aaagcgccgg gaagataccc aaaaggtaac tcgtattttt gggattcatc
      361 ataaaaccga agagctttgt gaagtttgtt ccgccgaatc agataaataa aaacggccag
      421 taacatgtag cacctggtca cataaccacg ggcattctgc aactgttcaa aataattaat
      481 aatgttttcc agatatacat catcataact tttcttatca gaaagattaa acaccatatc
      541 ttttaaagat tcgatatatt cttgtgattt atcatcaagt gacacaatat aacgagttaa
      601 tgctcctttg gggccactct caaactcatg aaaccgttca agtaatgctc ttgcataagg
      661 cgttgtgtca aaaatattaa aatcataaag atagcgtgca gcgtaaccat acaacccatg
      721 tacccaaagc ccttctctta tcagcactct gagataatat gcaatactgt caagtatctt
      781 taccttccgt tgcatatcaa gcaaaatact taatgaataa aatgcataat agaacttgtc
      841 aatgttgttg agtattctgt ccagctcact cttatcaagc ttcttctctc tgtaatcctt
      901 cttgtattca ttcttacagg cctttatata ttgtttcata aggctcttcc ttttaccgta
      961 cccttttcga atagttccct ggtattttcc cagctcatac atttttccgt tttcccgaaa
     1021 atcttctctg ttaaatccca gttcagcaag atactcccgg agataacgtt taacggcagc
     1081 ttcatcgctt tctgtaccag agtaatccag gcagggacaa tatgattttt gcaaaaaatt
     1141 atagatatga taataaaac
//
LOCUS       DAZUCC010000484         1144 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZUCC010000484 DAZUCC010000000
VERSION     DAZUCC010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.487"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..983)
                     /locus_tag="ACHY09_005307"
     CDS             complement(<1..983)
                     /locus_tag="ACHY09_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS3888105.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQAIQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQR"
     gene            complement(988..>1144)
                     /locus_tag="ACHY09_005308"
     CDS             complement(988..>1144)
                     /locus_tag="ACHY09_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFS3888106.1"
                     /translation="ELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEK
                     VTGQKDR"
BASE COUNT          324 a          334 c          250 g          236 t
ORIGIN      
        1 cgctgcgatg attcccgcat ggaggcatta atatgacgcg aaagcggtcg ctgcagtaat
       61 attcctgcca gaatcaccag cggaaccagt gtaagaggaa caacagcaag ccagcccccc
      121 acaagcccga tgacaccgat gaaaagcagg ataaacggca aatccacaaa agccagcagc
      181 gtggcgctgg tcataaactc cctgacagac tcatattccc gcaaattgct ggcgtatgaa
      241 ccggaagacg ccggcctgtc cgacattttc actgccatca cccgccttaa cagtgctgag
      301 ctgataatca ggtctgcctt cttacccgca atatccgtca gatagctccg gattaacttt
      361 gcagcacact caaacagaat ggctgcaatc accccgatac tcagcatcca taacgtttcc
      421 caggagcgat tgggtatgac ccggtcataa acgttcatga catacaggga actgaccagc
      481 gcaagaaagt taatgataaa actcgcaaga atgacctgtc cgtaatatgg cctgaaccgc
      541 cacagtaccc gccagaacca tgccccggtc atgtcatact ccggcagttc cgaacggcga
      601 tcctgtattg cctgtggctg aacaaaccag cagtaacctg aataaacagc cattaattca
      661 tcgtgagaaa gtgtcttacg gagtcccccg ggctggagta catgatactg ccgggccagg
      721 ccggcaccct caatacgcac caccacaatg gcttcctgtg cattcagtaa aagaagcaca
      781 ggaaccgcaa gtgaaggaat atcttccgga gcccgccggg aaagatgatt atcaaaccct
      841 tcactgcgaa gaaactcgcc aacagaacga aaatcaatcc cggagacatc tgtccggact
      901 gtaccggcag ccagcgtctc agcagataca ggtgtaccca gtaatgccgt tacctgtgat
      961 aatccttcaa aaatactctt cacttattta tctgtccttc tgccctgtta ctttttcttc
     1021 tgcaccagaa agattcactc cgctccattt caggagcatt ccctgggaat acagataatc
     1081 cagcattccg tggagcatct gatcctccgt ctgaacccgg gaaagcccga cactgtacag
     1141 ttcg
//
LOCUS       DAZUCC010000485         1123 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZUCC010000485 DAZUCC010000000
VERSION     DAZUCC010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.488"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(552..627)
                     /locus_tag="ACHY09_005309"
     tRNA            complement(552..627)
                     /locus_tag="ACHY09_005309"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(592..594),aa:Ile,seq:cat)
BASE COUNT          344 a          229 c          195 g          355 t
ORIGIN      
        1 tattagtgga attcgttttt cgagtgttag aaatttatat ctaaatagcg ttaattaatg
       61 agcaaagcca gactcctgta acgctcataa gactcatctg cctgcggcgg gtgttctggt
      121 cagtagtaga tatttaaggc gtggcagaga catttcatcc ttactctacg gcattggtcc
      181 acatacaaat cgtggttgtg acacttcact tatcatcagt gagcgaacag agaatagttc
      241 agtgatttga gtaattaacc tgattaaatg aagaggcatg ataaatgata atactctggc
      301 tttatcgtta attacttaat tccacatgta agcaatttgc ccgcttggca tagcgggcat
      361 tttttccagg tacttttgaa tgagtactga tggataaata cattgcagtg gcgtgccacg
      421 taccaaaaca ccagccctca ttcgaaacca cccaccgcac ttcttccttt aaatggcgtt
      481 agtcatgaaa tatagaccgc catcgagtac cccttgtacc cttaactctt cttgatacat
      541 gaatgatgat ttggtggccc ctgctggact tgaaccagcg accgggcgat tatgagtcgc
      601 ctgctctaac cactgaacta aagggcctta agtatgcaat aacaatactt ataaaccacg
      661 caataaacat gatgatctta tgatgtaaat aacagatttt tatgcgttct catccatcct
      721 gggtcgtcaa tacatgcgac ataaatcccg acaccgcttc cattcgcaaa gtcgatactc
      781 gcagtcaaca agcatgttaa taataagcac tatctatagt tatcatcgat tcaatgatag
      841 tttgtaatga ttttgtatct aataatataa ctttattaca ttagctgaag agttttcgca
      901 tcatgatgat atctgttact tttcactcca taaaaataaa cttcatatag caatatattc
      961 ttttatagaa cttattctgc taatcattag tttcgtatga gcgatttttg acagttgcct
     1021 ctccagacca aatcgataat taaaaaaaca gatttaagca ttatcctttt ccatatgaat
     1081 attggataaa agtaggacat ctgtttgcaa ttactttcac aac
//
LOCUS       DAZUCC010000486         1122 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZUCC010000486 DAZUCC010000000
VERSION     DAZUCC010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.489"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..708)
                     /locus_tag="ACHY09_005310"
     CDS             complement(<1..708)
                     /locus_tag="ACHY09_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFS3888107.1"
                     /translation="MKRILSFCFLFSVHNVFASPLPALLRDALIHDPSVMEARAEATS
                     ARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYDSEDILPGIRGEMNIFASGAIEAD
                     VSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALREKQSIAVLEQSLSRHNAILNDLN
                     TISIHDTGRESEFVQAEARRLMVRQQINTRTRVLKTTLGKLSTWTTNPVTEADLENPF
                     SRMTESRLLTDFTQTPQK"
BASE COUNT          343 a          242 c          257 g          280 t
ORIGIN      
        1 tttctgaggc gtctgcgtaa aatcagttaa caatctggac tctgtcatcc gggaaaaagg
       61 attttcaaga tcggcttcgg ttaccggatt tgtggtccag gtcgaaagtt ttcccagcgt
      121 ggttttgagc actcgggtgc gggtatttat ctgctgccgg accatcagcc tgcgggcttc
      181 ggcctgaaca aattcagatt cccgcccggt atcgtgaata ctgatggtgt taagatcatt
      241 aagaattgcg ttatgccggg agagactctg ttcaagtacc gcaatggatt gcttttccct
      301 tagcgcatca agatataatg aaacaaagga gcgaattgtt tcctcgccag cctcatcaac
      361 tttataacgg taatactcgg cttccgactc actccggctg acatccgcct caatagctcc
      421 cgaagcgaat atattcattt caccgcggat accgggcaaa atatcttcag agtcgtagtc
      481 atagtcgtag cggtgactct gggaaaggag tttactgcct gttgctgtga cgaccggcca
      541 gtgtgctgaa cgagcctgct ccacacggga acgggcagag gtggcttctg cccttgcctc
      601 catcactgat ggatcatgaa tgagagcatc cctcaggagg gccggcagtg gagaagcgaa
      661 cacgttgtgg acagagaaaa gaaaacagaa agataatatt ctcttcaccc gaacctccgg
      721 gacacgcggg aaatataatc aggcatcatg atgcaaccgg gtaaaatatt gagtaaaatt
      781 gaaatgcaga gacaccggaa ttgatccaag ctaccgccat tatcttccgc gctgacagaa
      841 gataataaaa atgtgagata cttcaatttt attgaaaagc tgaaaaaccc ttacaatcct
      901 tgcagtttat gaagaaaatt ttcacaaagt aaatttaaaa acaaagaaat ttaatgtaat
      961 ataatcgggg gagagttagc catcgatgat tgccagaaca aaaaacaata taaaccaaca
     1021 agatagaaaa tcatcacaac aaagggaaag gtctgtagat aaagagtggg atggacttct
     1081 tacttcatat cacataattg atatttttta tcctgcaaac tc
//
LOCUS       DAZUCC010000487         1120 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZUCC010000487 DAZUCC010000000
VERSION     DAZUCC010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.490"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..637
                     /locus_tag="ACHY09_005311"
     CDS             <1..637
                     /locus_tag="ACHY09_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFS3888108.1"
                     /translation="AVSVAIPGWQFLTQPSPAEQQLRALLAPLPDMLSADQMAQLSRT
                     PSLLNHASDWITLSGQQITRLTELPLTYNLQRSAQLLQQLMVLFPDNPRVQEMVDNWQ
                     KSVRSRALPEEAMTGWNEGMTRLQQLAERLNRLDEQRGKYMTVSELKTEVFGIMQAFN
                     RHIPAEEQLRRYDEVRNQNGSESQQKLAQDALMEQLNRYWLLRHGDAGNPA"
     gene            619..843
                     /locus_tag="ACHY09_005312"
                     /pseudo
     CDS             619..843
                     /locus_tag="ACHY09_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            846..1120
                     /locus_tag="ACHY09_005313"
                     /pseudo
     CDS             846..1120
                     /locus_tag="ACHY09_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          305 a          247 c          315 g          253 t
ORIGIN      
        1 ggcggtcagc gtggccattc cgggatggca gtttctgaca cagccatcac cggcggagca
       61 gcaactcagg gcgctgctgg caccactacc ggatatgctt tcagcagacc agatggcaca
      121 gctcagcaga acaccctcac tgttaaatca tgcgtcagac tggataacac tttcaggtca
      181 gcagataacg cgactgacag aactaccgct gacatacaac ctgcagcgta gtgcgcagct
      241 gttgcagcaa ctgatggtac ttttccccga taatcctcgt gtacaggaaa tggtggataa
      301 ctggcagaag agcgtaagga gtcgtgcgct cccggaagag gcgatgacgg gctggaatga
      361 aggcatgacc cgcttacagc agctggctga acgcctgaac cgcctggatg aacagcgggg
      421 aaaatacatg acggtcagtg aactgaaaac ggaggtgttt ggcatcatgc aggcttttaa
      481 ccggcacata ccggcagaag agcagttacg tcgctatgat gaagtccgta atcagaatgg
      541 cagcgaatct cagcagaaac tggcgcagga tgctctgatg gaacagttga accgctactg
      601 gcttctgaga catggagatg ctgggaatcc ggcgtgactt gggttcgcca accaccggaa
      661 gacgccatta cggatatggc gtattttccc ttttaccggt atgccatggc ctttgaagga
      721 ttcattccgg cattttcatc ttggtttgca ggtaaccggt gtggagtggc aatactgacc
      781 ggtgtccggg cggatgaatc cctcaactgc tttatggggc tggtgtctca gcgcaaactg
      841 agagaattac gtgggtagga tcagatgatg gtattggaac tgaaggtgat atgggaaaat
      901 ggttgttatt attttgtctg ctgtagctca gtctgaatga caacatatac ttgaaagaac
      961 aaatgaaggc cgtctcgaag ccgaagtaaa gggtatttaa tttgggcgaa aaagaacggt
     1021 caacagggaa caagtacaaa ccttacgaag tctgggtatc ggcgctacag aaatttcgcg
     1081 aaagatgaaa tttacacgtt ctactgtgta taaaatatta
//
LOCUS       DAZUCC010000488         1116 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZUCC010000488 DAZUCC010000000
VERSION     DAZUCC010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.491"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(126..951)
                     /locus_tag="ACHY09_005314"
                     /pseudo
     CDS             complement(126..951)
                     /locus_tag="ACHY09_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          267 a          280 c          256 g          313 t
ORIGIN      
        1 tccagaggtt tcacattagt ctccaatttt tcagaagaat ctatttacct ggcaggaaaa
       61 gtcatctggt gtttcgccgg agtgaggggt acaggcaata gcggacataa ccatttgttc
      121 tttttctaag cagccatctg ataatatttt atcctgaagg ctgtcgggga gatattcccc
      181 agacgagagt gacgacgctg acgattgtag aaaatctcaa tgtattcccg tattactgag
      241 atggcctcat cccggctttt aaaacgatag tggctcagac tttcattttt cagcgttccc
      301 cagaagcttt ccataggagc attgtcgtaa cagttaactt tacgcgacat tgatattttc
      361 agaccaaact gctcctgtat ggccctgtaa tcgtatgcgc agtactgtga accacgatcg
      421 gagtggtgga ttagcccggt aggtgagcgc tggttcctga gcgccataaa cagggcttta
      481 cccgtcaact atttcgtcat gcgctctccc atggcgtaac cgacaatttt gctcgtataa
      541 acatctttga cgccagcgat atacagccat ccttccgggg ggcaacatac gttatatctg
      601 caaccaagac ctgattaggt gctgtaggcg taaagttctg attcaggaga ttcggcgcaa
      661 ccggcagatt gtgacaagag ttcgtgttcg ccctgaactt gcgtttctgc ttacagtgca
      721 gcctcagctc cttacgaaga cgtgccagtc tgtcacgacc gacgataatt ccgttatctg
      781 ccagctccgt ctggagcagc taggttccat atgtttcgcg tgtgcggata tgtgccacct
      841 taatctccag ttttagccgc tcatcacttt gttttctgtc tgagggtcat gctgtactca
      901 gtcgtaataa ccgatcctgt atacaccaag tacccgacac atcgcttcaa tacataatcg
      961 catctgaatc gttcaccgat tcgcggcatt tttcaaggta gttgtcgcca ttgttcgact
     1021 aactgatcga cattacagcc tagagcctag ctgggggctg tgcacaaaaa agccccggac
     1081 attccggggc tacactgcat attaacgaac gctctg
//
LOCUS       DAZUCC010000489         1113 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZUCC010000489 DAZUCC010000000
VERSION     DAZUCC010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.492"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1113
                     /locus_tag="ACHY09_005315"
     CDS             <1..>1113
                     /locus_tag="ACHY09_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFS3888109.1"
                     /translation="AERLRSQLVLQQAIARLQHREATGAPWYTRFGLNQDSDTLAALW
                     PLYAKNNAQLMRDATADSLRQQLNAFVQLPPASDARTQGTQHAYDILKSYLMLARPDK
                     ADAGWLAKNALKALPKRRNVPDGIWQNVAPKLLGFYAQNLPAHPEWKIKPDDELISTV
                     RQILLKQIGQRNAESGLYQEMLKRVASNWPDLTLADMTGDTDASTVFSTDEVVPGMFT
                     RQAWEEQVQDAINEVVRTRRDEIDWVLTDKTHQTGSDISPEALKSRLTERYFTDFGNA
                     WLNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSPLVALMNTLNYQGKTGQKGEALADSV
                     VDSAKKLLDKNKNGRQFIEQTQGPEGPLDGVFGPLTG"
BASE COUNT          285 a          287 c          317 g          224 t
ORIGIN      
        1 ggctgaacgc ctgcgcagcc agcttgtact ccagcaggcc attgcccgtc tgcaacatcg
       61 tgaggcaacc ggtgcgccct ggtacacacg atttggtctt aatcaggaca gcgacacgct
      121 ggccgcgctc tggccgctgt atgcgaaaaa taacgcacag ctgatgaggg atgccacagc
      181 agacagcctc aggcagcagc tgaatgcctt tgtgcaactc cctcctgcca gtgatgcccg
      241 tacgcagggc acacagcatg cttacgacat actcaaaagt tatctgatgc tggcgcgccc
      301 ggacaaagcg gacgccggct ggctggcaaa aaatgcgctg aaagccttac cgaaacgccg
      361 gaatgtgccg gatggcatat ggcagaacgt cgcaccgaaa ctgctggggt tttatgcgca
      421 aaaccttccg gctcatccgg aatggaaaat taagcctgac gatgaattaa tcagtacggt
      481 ccgtcagatt ctgcttaagc aaattggcca gcggaatgct gagtccgggt tatatcagga
      541 gatgcttaag cgcgtggcca gtaactggcc tgatttaaca cttgcggata tgaccggtga
      601 caccgatgcc tcaacggtat tcagcactga cgaagtcgtt ccgggaatgt ttacgcgtca
      661 ggcctgggaa gagcaggtac aggatgcgat taacgaagtt gtcagaacac gccgcgatga
      721 aattgactgg gtactgacgg ataaaacaca tcagacaggg agcgatatct ctccggaggc
      781 cctgaagagc cgcctgactg aacgctattt taccgatttt ggtaatgcct ggctgaacat
      841 gctcaacagc atacagtggc atgaacccac gtcactttcc ggagccattg cccagttgag
      901 tctgctcggt gacgtccgcc agtctccgtt agttgcactg atgaacacgc tgaactacca
      961 ggggaaaacg gggcagaagg gggaggcact ggctgattca gtcgtggatt cagcgaaaaa
     1021 gctgctcgat aagaataaaa atggcaggca gtttattgaa cagacacagg gccctgaagg
     1081 tccgctcgat ggggtatttg gaccgctgac agg
//
LOCUS       DAZUCC010000490         1105 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZUCC010000490 DAZUCC010000000
VERSION     DAZUCC010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.493"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            16..1068
                     /gene="ybhH"
                     /locus_tag="ACHY09_005316"
     CDS             16..1068
                     /gene="ybhH"
                     /locus_tag="ACHY09_005316"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFS3888110.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLASIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISCSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT          284 a          249 c          281 g          291 t
ORIGIN      
        1 attgagggca ttattatgaa aaaaataccc tgcgtgatga tgcgaggtgg aacctcgagg
       61 ggcgcgttcc tgttagcgga acatctaccc gaagatcaaa cgcagcgcga taaaatattg
      121 atggcaatta tgggttccgg taacgatctg gaaattgacg gtattggcgg cggtaatccg
      181 ctgaccagta aagtcgccat tattagccgc tccagcgatc cgcgtgcaga tgtcgattat
      241 ctgtttgctc aagttatcgt ccatgagcaa cgtgtcgata ccacgcctaa ctgcggcaat
      301 atgctgtctg gcgttggggc attcgccatt gaaaatggtt tgattgcagc gacttcgcca
      361 gttacccgcg tacgtatccg caacgtcaat acgggtacgt ttatcgaagc tgatgtgcaa
      421 acgccaaatg gtgttgtcga gtacgagggt agcgccagaa ttgacggcgt accgggtact
      481 gccgcaccgg ttgcgctcac tttcctgaat gccgccggaa cgaaaaccgg aaaagtcttc
      541 ccgacggata atcagattga ttattttgac gatgtcccgg tgacctgtat cgatatggcg
      601 atgccagttg tcattattcc tgctgaatat ctgggaaaaa caggctatga attaccggca
      661 gagctggatg ccgacaaagc attattagcc agcattgaat ctatccgtct acaagcgggt
      721 aaagcaatgg gcttaggcga tgtcagtaat atggttatcc ctaaacctgt acttatttct
      781 ccagcgcaga aaggcggggc aattaatgtg cgttatttta tgccgcattc ttgccatcgc
      841 gctttggcga taaccggtgc tattgctatt tcctgtagtt gtgcattgga aggcaccgtc
      901 acccgacaaa tcgtcccttc tgtaggatac ggcaatatca atattgaaca ccccagtggt
      961 gcgctcgacg ttcatttaag taatgaaggt caggatgcca cgacgttacg cgcatctgtt
     1021 attcggacga caagaaaaat attttccggt gaagtttatc ttccctgaaa aattttgttg
     1081 tcgtgataat gacaatcaat aaagg
//
LOCUS       DAZUCC010000491         1099 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZUCC010000491 DAZUCC010000000
VERSION     DAZUCC010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.494"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1099
                     /locus_tag="ACHY09_005317"
     CDS             <1..>1099
                     /locus_tag="ACHY09_005317"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFS3888111.1"
                     /translation="VTLSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEIKDN
                     NGNAVGTLDVPMIDFSSVSRRGSLTLLSQGYGVSAKHGDLGDVNNASFGYDKNNYTVV
                     KNNKHSGLDFSLHRFSKLIAEATPADINISGQLSDSSQYTAFYRAGAGTQYIKERSGK
                     QTHIPGTFLTGGTVGTPWYSGNDLISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDS
                     LSEKWSLAGVTLHNYGVNGYRNNWLLLPEDYIRNIIVADFDPIISFNKNSKEHMSWTY
                     DAAKGVGRIQQDDQQFVMHGNLNGNLNAGKNLYFTGENGIIDLKDNVNQGAGYLQFAD
                     DYTVTTSNDSSWSGGGIIVNYGTTVKWGINGV"
BASE COUNT          339 a          197 c          254 g          309 t
ORIGIN      
        1 gtgactcttt ctgctttacc gggtaaagca tcaacggtca gtgcagaaat accatatcag
       61 acttttcgtg actttgctga aaataaaggt gtgtttaccc ccggagtgac aggaattgaa
      121 ataaaagata ataatggaaa tgcagttggt acgcttgatg tgccaatgat tgacttcagc
      181 agcgtgtcac ggcggggctc actgaccctg ttgtcgcagg gatatggtgt atcagcaaaa
      241 cacggagacc ttggtgatgt aaacaatgcc agttttggct atgataaaaa taattatact
      301 gtagtaaaga acaacaaaca ttcgggcctt gatttttctc tgcatcggtt cagcaaactc
      361 atcgcagagg ctacacctgc tgatataaat atatctggac aacttagtga ttcgtcacag
      421 tatactgctt tttacagagc aggcgctggc acacagtata ttaaagaacg ctcaggtaaa
      481 cagactcata tcccgggaac atttttaact ggtggaacag taggcactcc atggtattca
      541 ggaaatgatt taatatcaag ctctccaggt gacacctata ataaatcaca gggaccatta
      601 gccagttatg ggcagatggg tgacagcggg tcgccattat ttgcttatga ttctctcagt
      661 gaaaaatggt ctctggctgg agtcaccctg cataactatg gggtgaacgg ttacagaaat
      721 aactggttgt tattacctga ggattatatc agaaatatta ttgtagccga tttcgatccc
      781 attatttcat ttaataaaaa cagtaaagaa catatgtcat ggacatatga tgcagccaaa
      841 ggcgtgggcc gtattcagca ggatgaccaa cagtttgtga tgcatggtaa cctgaatggt
      901 aatctgaatg caggtaaaaa tctttatttt acaggcgaaa atggcattat tgatttaaaa
      961 gataatgtta atcagggtgc cggatatctt cagtttgctg atgattatac agtaacaacg
     1021 agcaatgaca gttcatggtc cggaggaggc attattgtca attatggtac aactgtaaaa
     1081 tggggtataa acggggttt
//
LOCUS       DAZUCC010000492         1098 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZUCC010000492 DAZUCC010000000
VERSION     DAZUCC010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.495"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..>1098
                     /locus_tag="ACHY09_005318"
     CDS             17..>1098
                     /locus_tag="ACHY09_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS3888112.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLTGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFG"
BASE COUNT          230 a          238 c          295 g          335 t
ORIGIN      
        1 gtaagctggg ggcgatatgg atttctttcg ttttttgatg agcgatgtac tttccgaacc
       61 cgcagtgctg gtcggtttaa tcgccctgat tggcctgatt gcacagaaaa aaccggtaac
      121 cgaatgcatt aaaggtaccg tcaagaccat tatggggttt gtgattttag gcgcaggggc
      181 gggtttagtt gtgtcgtcac tgggtgactt cgcaaacatt ttccagcatg cctttggtat
      241 tcagggtgta gtgccgaaca atgaagccat tgtttccgta gcgcagaaaa gcttcgggaa
      301 agaaatggcg atgattatgt tctttgcgat ggttatcaac attatgattg cccgtttcac
      361 accgtggaaa tttatcttcc tgaccggtca tcacacgttg tttatgtcga tgatggtagc
      421 ggtaattctg gcaacagcag gcatgaccgg cattacgctt atagccgttg gctctttggt
      481 tgttggcgtg gcaatggtct ttttcccggc tattgcgcat ccgtacatga agaaagtgac
      541 cggttctgat gacgttgcca tcggccactt ctcaacatta tcttatgtat tggccggttt
      601 catcggcagc aagtttggta ataaagagca ctcgactgaa gacatgaatg tgccgaaaag
      661 tctgctgttc ttgcgcgaca cgccggttgc tatctctttt accatgagca taattttcct
      721 ggtgacgtgc ctgtttgcgg gtgcagatgc ggtgaaagag cttagcggtg gtaaaaactg
      781 gttcatgttc tctatcatgc aatccattac cttcgcagct ggcgtgtaca tcattctgca
      841 gggtgtgcgc atggtgattg cggagattgt cccggcattc aaaggtattt cagacaagct
      901 ggttccgaat gccagacctg ctctcgattg cccggtcgtc ttcccttacg cacctaacgc
      961 tgtactggtt ggtttcctga gtagctttgc tgcaggttta accggtatgt tcacgttgta
     1021 tctgctgaac atgatcgtga ttattcctgg tgtggtgccg cacttcttcg taggtgcagc
     1081 tgcaggggta ttcggtaa
//
LOCUS       DAZUCC010000493         1094 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZUCC010000493 DAZUCC010000000
VERSION     DAZUCC010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.496"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            287..1094
                     /locus_tag="ACHY09_005319"
                     /pseudo
     CDS             287..1094
                     /locus_tag="ACHY09_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          313 a          252 c          276 g          253 t
ORIGIN      
        1 gttttgaagt aagtctgtgg tttaggaagc ccctatcctt gcgttgtgtt acatttagga
       61 atttcctagt agaaagagcc tcacaactca aggatactgt acaattcaga taaatcactt
      121 aacttgatgc atcaatcacc cagcgaccag ttatgctcat cataatcatt attcaggtgt
      181 gagcataatg agcataaccc agcgatccag acaggttccc tgaatctcca gacaaccaat
      241 atcaattaaa taagtgatag tctaaaaact agtcattaga gaacagatga ttgatgtctt
      301 agggccggag aaacgcggac ggcgtaccac acaggaaaag atcgctattg ttcagcagaa
      361 ctttgaaccg ggaataactg tctccctcgt tgcacggcaa cacggtgtga cagccagtta
      421 tttctctggc gtaagcaata ccaggaagga aatctcactg ctgtggctgc tggagaacag
      481 gttgttctgg cctctgagct tgctgccgcc atgaagcaca ttaaagaact ccagcgcctg
      541 cccggcaaga aaacgatgga aaattaactc attagagaag ccgttgaata ttggcgtgca
      601 aaaaagtgga tagcgcacgc ccaatattgc ccgggaatgg ggagtaagtt tcgtcagccg
      661 ttatctccgt gtgttgcgtg cgcagttgca cgtcattctc agacgaaccg atgactggaa
      721 ggatggccgc cgcatgatac ggatgtttat ctccgtaata acatggtgta caccatgtta
      781 tcggagagct gcccacgtat ggttatcgtc ggctgtgaaa cagtacagga cgtcatgctg
      841 ggagcggtag aacaccgctt cggcagcgac cttcctgcgt ctccagtgga gtgcctgacg
      901 gataatagtt catgctatca ggctaatgaa acacgccagt tcgcccggat gttggaactt
      961 gaaccgaaga acacggcggt gcggtatccg gagagtaacg gcatagcaga gagcttcgtg
     1021 aaaacgataa agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca
     1081 aagaaccttg caga
//
LOCUS       DAZUCC010000494         1094 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZUCC010000494 DAZUCC010000000
VERSION     DAZUCC010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.497"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..764)
                     /locus_tag="ACHY09_005320"
     CDS             complement(<1..764)
                     /locus_tag="ACHY09_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3888113.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSFSGTVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPSLENSHPTKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNISYQKLRWARKNEIVTVYNPGPRYVTLYNLHVDGKVMDGGMIAPFSHRQQSWCK
                     AQGICEITWQTLDIYNNVLPVWKVNMNPLQMDVSGS"
     gene            complement(730..>1094)
                     /locus_tag="ACHY09_005321"
     CDS             complement(730..>1094)
                     /locus_tag="ACHY09_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3888114.1"
                     /translation="PDVPSSSVRSAGDVTEKVYFSITLTRCGSDVGNAYIKFTGNTVS
                     EDASLYKLEDGSVDGLALTIFDKNKGSISNDVKSIGFSLTSSVDNILHFFAAYKALKN
                     NVQPGDANASVSFIVTYD"
BASE COUNT          332 a          232 c          198 g          332 t
ORIGIN      
        1 tgtgagcctg acacatccat ttgcaacgga ttcatattta ctttccatac aggcaataca
       61 ttattataaa tgtccaatgt ctgccatgtt atttcacaaa tcccttgcgc tttacaccat
      121 gattgttgac gatggctaaa tggtgcgatc ataccaccat ccataacttt accatcaaca
      181 tgaaggttat agagtgtcac atatctgggg cctggattat aaacggtaac aatttcattt
      241 tttcgtgccc agcgtaattt ttgatatgaa atattcgggt cttcagacaa atttgatggc
      301 cgatagaata ttttcatacg attgcggacc gctatttgca attgattatc agttttggtt
      361 ggatgactat tttccaacga tggtaatgca gcaacattaa gtataaataa actttcccgg
      421 tcggcaggta aactgccacc aacacgatta attcttattt ttgtattttt gtcaggcttc
      481 aaaacaaaca gtggcggagt tgcgataaat accgttccag aaaatgaggg caaagattca
      541 accgttcccg tcaactgacg agcatcatca ggggagactg caaccttgac taacatatct
      601 ctgtctgaag tgtttttgag ttcaacagat attgaggact gattttccgg atacgcaaaa
      661 cgcgttccac cgatcactaa tcctgcttgt gctgtagaga ataaaaggga aaataatact
      721 atgactacct taatcatagg tgacaataaa cgatactgac gcattggcat cccctggttg
      781 gacattattt tttaatgctt tgtaagccgc aaaaaaatgc aatatattat caactgatga
      841 tgtaagtgaa aaacctatgc ttttaacatc attactaata ctgcctttgt tcttatcaaa
      901 aatcgtgagt gcaagcccat ctaccgagcc atcttccagc ttatataaac tggcatcttc
      961 agaaactgta ttgccggtaa actttatata cgcgttgcca acatcactac cacagcgggt
     1021 taacgttatg gaaaaataaa ccttttcagt aacatcccct gcactcctta ccgaactact
     1081 gggtacgtct ggca
//
LOCUS       DAZUCC010000495         1084 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZUCC010000495 DAZUCC010000000
VERSION     DAZUCC010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.498"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            68..>1084
                     /locus_tag="ACHY09_005322"
     CDS             68..>1084
                     /locus_tag="ACHY09_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFS3888115.1"
                     /translation="MKIRYAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDA
                     KQQPFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNIS
                     SDKESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSID
                     ADGRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVMALNDHQLLVIERSGRNVSAGFN
                     DWDYSVRVYMVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGV
                     TFGPL"
BASE COUNT          324 a          226 c          232 g          302 t
ORIGIN      
        1 accatcggag gttaatcttt ttctttcaca acacgactaa aatcatatat atacaaaggt
       61 aaatatcatg aaaataagat atgccgctat ttttctggca ttaccgctgt tatgcacaac
      121 agcgcaggca aacagttttc ttcattatgc tggggagttt aacttccgga cgggagaaaa
      181 gcagaacaat gtcacaatcg cggggctatc agccatcaca tttgatgcaa aaaaaaatat
      241 tttctatgct attaacgata gcagaaacaa taataaagaa ggagatgcca gtctttatac
      301 actaaaaata caggtatcat ccaggggcat tgagcaagtg aattttctca accagcgtcc
      361 actgcttgat gcaaaacagc aacctttcgt gaccaatacg gtggatgccg aaggtctggc
      421 cctgacacac aatggtaaat cattactctg gagttcggag ttaggtgctc cactgcgtct
      481 gagcactctc gatggcgtaa tggagaaaga ttttacgtct ttatttcctg ctcgctttaa
      541 tattagttct gacaaagaga gcagcaatgg tattcgcagc ggcaacgcct gggagggcct
      601 gacagttaca ccagatggaa aatcactttt tatcgccgtt gaaagcagcc ttaaacagga
      661 tgggcctatt gcaagtccaa taaattcagg cacatcacgc ttactgcaat tttctataga
      721 tgcagacggt cgaccttcaa agcaattgca tgaatatctc tatattactg atcctgttcc
      781 gcaagtgagt aagttcggta ttaatgataa tggcgtatcc gaggtcatgg cgctgaatga
      841 tcaccaattg ttggtcatcg aacgttccgg cagaaacgtc agtgctgggt tcaatgactg
      901 ggattactct gtcagagttt atatggtcga tttaactgct gccagcgata tcaagaatat
      961 tgacagtctg caagattggt ccaataaatc cacgttacaa cccgtcagta aaaaattatt
     1021 aattgatttt gctgattaca cttcctctgc ggattgtatt gaaggggtaa ccttcggtcc
     1081 tctg
//
LOCUS       DAZUCC010000496         1074 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZUCC010000496 DAZUCC010000000
VERSION     DAZUCC010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.499"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            682..>1074
                     /locus_tag="ACHY09_005323"
     CDS             682..>1074
                     /locus_tag="ACHY09_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3888116.1"
                     /translation="MKRSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPAAHLL
                     VLLGIFGYIMHRTMPGISFPVFLLNGLIPFFIFNSISNRSVEAIDANQGLFNYRPVKP
                     MDTIIARTLLETLIYIIVYVLLMLIVWMS"
BASE COUNT          317 a          186 c          235 g          336 t
ORIGIN      
        1 taagagtaag ctattagatg taaattactt tggtcaggtc cttcacagca gaaacagaat
       61 gtcatgaaag ataaatttac gcagtcactt gatcaactgc ttagttttgc gtcttaaagg
      121 attgtaacta tggcaactgt ggagcgaaaa atcattgggc acgggaaaag tgcggtcata
      181 tagaaaagtg ctctctattc agcatctcat ggtttatcag cgaagacaga aaaaaattat
      241 aacgatgcgc aggaatttgt ggaggtttgc taatatagag cgattcgagc atccctcgtt
      301 aaatctctcg aaccgcagta tattcagaca tttttaaaat acgccagatg attacgacag
      361 aaagaacaca acatataatt catgttcgct gtcctcttgc tgaatacgga attgtcttca
      421 ataaaggtgc tacacattat ggtgagagct accacggttt cttgaggatg cagaaaatgg
      481 atttaccgat atcgtgcaat tttgtatcgg tagtatgtcc gactcatgtc tcatggataa
      541 tgaaccatat attataactg cgggtagaaa aatgcgcatc taacgacttt gcctggacgt
      601 gtcgattaac tgttcctggg tttgctactc ttgtcagtca gacgataaaa gtgagcataa
      661 atatatagga ttacatatca agtgaaaaga agtggatttg aagttcagaa ggtgacagta
      721 gaggcattat ttctacgaga aatacgaaca cgttttggta agtttcgtct gggatatctg
      781 tgggcaatcc tggaacccgc tgctcatttg ctggtgctgt taggtatttt tggctatatt
      841 atgcatcgta cgatgccagg tatctcgttc ccggtatttt tacttaatgg tttgattccc
      901 ttttttatct ttaatagtat cagtaatcgg tctgtggaag ctattgatgc aaaccagggg
      961 ctttttaatt atcggccagt aaagcccatg gatacaataa tagccagaac cctgcttgag
     1021 acactaattt acattatagt ttatgtgttg cttatgctta ttgtctggat gtca
//
LOCUS       DAZUCC010000497         1073 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZUCC010000497 DAZUCC010000000
VERSION     DAZUCC010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.500"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..299)
                     /locus_tag="ACHY09_005324"
     CDS             complement(<1..299)
                     /locus_tag="ACHY09_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS3888117.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTN"
     gene            complement(380..922)
                     /locus_tag="ACHY09_005325"
     CDS             complement(380..922)
                     /locus_tag="ACHY09_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3888118.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDISAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          320 a          226 c          195 g          332 t
ORIGIN      
        1 ttattagtgg gtagtttatt cggcataatt ttaattttta cttgctgccc ggaatttgcc
       61 cctatttttg ccactggtgg cgttaacatg aaaggaacct gaattttttc tggtggtaat
      121 gacgtatcgc catcatcaat ccacgcctgc agcagcgaag aacggtttcc ctggttcatc
      181 aactgcacca tcacttcttt attttctgcc gggtaaataa tgcgcgtccc gtagataaca
      241 ataccggcat gtgcaggaag gaccatagaa gaaatgagta aagctaataa tcctttcatc
      301 ttaatgcctc ttaaattaaa aggccgccat ggggatagcg gccatgttta ttacctggag
      361 aaacttgcaa ttttatttat taatcctgcg ccacacgcat aaccagagaa gataacacat
      421 cacctgattg cactgaatta tctgtcagct tcaggtattt tgctttaaac ggaatagcat
      481 attgaccttc cgctgtttta taactctcaa tgttaaaagg tgcagaaata tccaggacat
      541 cactgttatc acctaaatag agatagtaac ccacggtatc gttgctactg gctatcgcct
      601 tatcgttgcc ggttattttg tcgtttgtga tcaacgagag ttttacacca gcaacttctt
      661 tcgggcagct aatttttaac atgctgctgg ttacaccgac tgcatcatca taggtagaat
      721 atttagcttt accttcaaag acagtggttg gtacagttgg catattaata cttactgaat
      781 cgccacctgc ttcgaattta cacggagatt ctgcaatatt ccctttaatg gttaatgttc
      841 cggtatcaac gtctgcggca aataccccag cgctcataaa aaaagaagaa aaaacagcgg
      901 cagcaataat tgaacgtttc ataatatgct ccttaataaa atctattttt ggttgttaag
      961 agtctgtatc tcttggaaac aaatttctca cacccaaaaa gttgccgcaa tgtaaacaca
     1021 ttgttttatt gatttaaagc aaattaacca ttgcaatata aacacaacaa ata
//
LOCUS       DAZUCC010000498         1062 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZUCC010000498 DAZUCC010000000
VERSION     DAZUCC010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.501"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1062
                     /locus_tag="ACHY09_005326"
     rRNA            <1..>1062
                     /locus_tag="ACHY09_005326"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          290 a          227 c          336 g          209 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 aagtgttgtt gggtagggga gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct
      661 ggaggtatca gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct
      721 cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa
      781 ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac
      841 tgcgaagggg ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt
      901 gtaggctggt tttccaggca aatccggaaa atcaaggctg aggcgtgatg acgaggcact
      961 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
     1021 caaatcgtac cccaaaccga cacaggtggt caggtagaga at
//
LOCUS       DAZUCC010000499         1054 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZUCC010000499 DAZUCC010000000
VERSION     DAZUCC010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.502"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1054)
                     /locus_tag="ACHY09_005327"
     CDS             complement(<1..>1054)
                     /locus_tag="ACHY09_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFS3888119.1"
                     /translation="AVKPDVLRFRGREALSEPFRWDIGFTSRNANIPPEEVLMKYASL
                     RMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLALLEYTRQCAVFQNQSVPEVVEQ
                     VLRKHGLEGADFEFRLERAYPSREIITQWQETDLQFIQRILSEVGIFWRTEMDDVRGL
                     DTYIFADSQLNYQFDVCLPYSEPSGLFDGAAESVWDVRTWHNVAIGTVSTRDYNYRTA
                     SMPMDATVSVRNDAVTTGEHYRDAPAYREAGDDTSPEPETESGAFYARLHHERELNRS
                     ARIHLFSNAAHLSPGQVLEPQGDVIADLKEGIVLTLVTYRGARDSRLHVSAWGMPYTE
                     RYCFRPAEIPRPVIPGT"
BASE COUNT          227 a          322 c          291 g          214 t
ORIGIN      
        1 gcgtacccgg gatgaccgga cgcgggattt ctgccggacg gaaacagtaa cgttcggtgt
       61 aaggcattcc ccatgctgac acatgcagcc gggaatcccg ggccccccgg taagtcacca
      121 gtgtgagaac aatcccttcc ttcaggtccg caatgacatc cccctgcggc tccagcacct
      181 gcccggggga gaggtgtgcg gcgttgctga acaggtgaat gcgggccgac ctgttgagtt
      241 cccgctcgtg gtgaagccgg gcgtaaaacg caccagattc ggtttccggc tccgggctgg
      301 tgtcatcccc cgcttcacga taagccggtg catcgcggta atgctccccg gtggtgaccg
      361 catcattacg cacgctgacc gtcgcatcca tcggcatgga ggcagtccgg tagttgtaat
      421 cacgcgtgga gaccgtgccg atggcgacgt tgtgccatgt ccgtacatcc cagaccgatt
      481 ctgcagcacc gtcaaaaagg cccgacggct cgctgtacgg cagacacaca tcaaactggt
      541 agttaagctg gctgtcggca aaaatgtacg tatccagtcc gcgcacatca tccatctccg
      601 tgcgccagaa aatccccacc tcagacagaa tgcgctgaat gaactgcaaa tccgtttcct
      661 gccactgggt gataatttca cgcgacgggt aagcgcgttc cagtctgaat tcaaaatcag
      721 cgccctccag cccgtgtttg cgcagcacct gctccaccac ctcagggacg gactggttct
      781 gaaatacggc acactgccgg gtgtactcca gcagtgccag acgggagctg agtgtaagcc
      841 ggtagtggga ctggtctttc gatgtggaca gccattccag acgggtcaca atgccatgga
      901 catttttgcc gttacgcatc cgcaacgacg catatttcat cagcacctct tccggaggaa
      961 tgttcgcatt ccgggaggtg aacccgatgt cccatcggaa cggctcactc agtgcttccc
     1021 tgccacgaaa acgtaaaaca tcaggcttta ctgc
//
LOCUS       DAZUCC010000500         1053 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZUCC010000500 DAZUCC010000000
VERSION     DAZUCC010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.503"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(378..>1053)
                     /locus_tag="ACHY09_005328"
     CDS             complement(378..>1053)
                     /locus_tag="ACHY09_005328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888120.1"
                     /translation="LLVSGLADSRTRGLADSRTRGLADSRTRGLISYELTPQEYNAIE
                     LLNSNNLMWGTYNLKKLFGPAIRGKRLKNADRIPGSLPFVTAGEGNEGISAFISNDVV
                     VFPENTITIDMFGSAKYRNYEYGADDHVAVVHTEFFDRNAVIFITASIHKSSCTGKFN
                     YGRNFYAKDADELMVSLPSKDNNPDYSFMETLILAVHKLVIKDVVQYTDKKLVAYRQI
                     TSEKVV"
BASE COUNT          292 a          235 c          216 g          310 t
ORIGIN      
        1 gctgctttga cgctacggac ataaaaaagc cagccactgg gggaggctgg caaactcgta
       61 gagcaaaatg ctgttacgca aacttcgtta cagggttatc ctgcaatact taaaatatac
      121 aatatttaga aaactaatag tgccatatgc gatttttaag attttgttat taattgcggt
      181 cgcaccttcc tttctgtgta ctttccgtat aacttacagg attctggata caaaaaaccc
      241 gcgcatcggc gggttaagca gcgtggcaat gtaaccactt ttgtcatgat atgatgattt
      301 ttacgattgt aaactgtttt tctgcatcag ggcacgataa cctgaccatt ccccaccctg
      361 tcaggtgggg atttgcacta aacaaccttc tcagatgtaa tctgcctgta cgctaccagc
      421 tttttatcag tgtactggac aacatctttg atgaccagtt tatgtactgc tagaattagc
      481 gtctccatga aagaataatc agggttatta tcttttgacg gcaaagatac cattaactcg
      541 tcggcatctt ttgcatagaa atttctcccg tagttgaact ttccggtaca agaagactta
      601 tgaattgatg ctgttatgaa aataacggca tttctgtcaa agaattctgt atgaacaact
      661 gccacatggt catctgcacc atactcataa tttctgtatt ttgcggagcc aaacatatct
      721 atcgtaatgg tattttcagg gaatacaaca acatcattac tgataaatgc agaaatacct
      781 tcattgcctt cacccgcagt aacaaaaggc aaggagccgg gtatacgatc tgcgtttttc
      841 agacgcttac ctcttattgc ggggccaaat aactttttga ggttataagt gccccacata
      901 agattattgc tatttaatag ctcaatcgcg ttatattcct gtggtgtaag ctcatagctt
      961 atcagtccgc gagtccgcga gtccgcgagt ccgcgagtcc gcgagtccgc gagtccgcga
     1021 gtccgcgagt ccgcgagtcc gctaaccagt aga
//
LOCUS       DAZUCC010000501         1052 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZUCC010000501 DAZUCC010000000
VERSION     DAZUCC010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.504"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..>1052
                     /locus_tag="ACHY09_005329"
     CDS             5..>1052
                     /locus_tag="ACHY09_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFS3888121.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQ
                     VQGLNGTADVLDPESGKKVGEKTVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSET
                     QNMTTFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVK
                     YIDVGATYYFNKNMS"
BASE COUNT          275 a          197 c          287 g          293 t
ORIGIN      
        1 aatcatgaaa aaaatgacag tggcactttc tgctgtagca gtggcagtga tgtttgctgc
       61 gggggcgcag gcagcagaag tttataataa agatggtaat aaactggatc tttacgggcg
      121 tgcaaccgct ctgcattact tctcggatga taaaggtaat gacggagacc agacttatgc
      181 tcgtctcggc tttaaaggcg aaacgcagat taatgatcaa ctgaccggat ttggtcagtg
      241 ggaataccag ttctctggta acaaaacgga atctgaaggt tccgcgggaa ataaaacccg
      301 tctggcattt gcaggtctga gatttgcaga tgttggtacc attgattacg gacgtaacta
      361 tggtattgct tacgatgtcg gatcatatac tgacgtactg cctgagtttg gtggtgatgg
      421 ctggacgcag accgataact ttatgacggc ccgaacttcc ggtgtcctga cttaccgtaa
      481 tacagacttc ttcggactgg ttgatggtct gaattttgcg gcgcagtatc agggcaaaaa
      541 tgagcgtgat gaccttcaga aggctaatgg cgacgggtat ggtttctcgg ccagctatga
      601 gtttgatggt tttggttttg tcgctgcgta taccaagtca gatcgtaccg ataagcaggt
      661 tcaggggttg aacggtactg cagatgttct tgatcctgaa tcaggtaaaa aggttggaga
      721 aaaaacagtt gactcaggta gtgtggcaaa aggtaaacat gctgaattct ggggaactgg
      781 tcttaaatat gatgccaata acctgtatct ggcagcagtg tattctgaaa cccagaatat
      841 gacgaccttt ggtgatcaag gtgttgcgga taaagctcag aatatcgaag ccgttgttca
      901 gtatcagttt gattttggtc tgcgcccgtc ccttgcctac cttcagtccc gcggacagga
      961 tgttatggtt ggtggtgtga accatggcga tcaggatctg gttaaatata ttgatgtcgg
     1021 tgcgacttat tactttaaca agaatatgtc ca
//
LOCUS       DAZUCC010000502         1050 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZUCC010000502 DAZUCC010000000
VERSION     DAZUCC010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.505"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..485
                     /locus_tag="ACHY09_005330"
     CDS             204..485
                     /locus_tag="ACHY09_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFS3888122.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTDFIIAAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
     gene            491..979
                     /locus_tag="ACHY09_005331"
     CDS             491..979
                     /locus_tag="ACHY09_005331"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3888123.1"
                     /translation="MFQLTPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHHIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
BASE COUNT          304 a          239 c          230 g          277 t
ORIGIN      
        1 actccggatg tcactgctgg taaataatct gagcttttat ctgtatgcaa tggtcaaaga
       61 tcgtggcgtt aacatccccc ggatagtttt gtctgaatct gatttttctt ttcaggcaga
      121 atgtctgtct gccgtcccgg tgtcagagtg taatttgcac aatacggcaa tttgccgtac
      181 agtgtattca ggaggtacaa atcatgccat ctgcaacaca aactgcccga ctggaagcaa
      241 ggatcagcac agaactacat gccatgctga agcgcgctgc agaaatccag gggcgcacta
      301 tgaccgattt tatcattgct gccgttcagg atgcggccca acgcgctatc gaacaaaccg
      361 atataatccg actgtcccgg acagatcagg aaatttttgc tcaggcatta ctgtcaccaa
      421 cagagccgaa tgatgcgctt aaacgggcct ttaaacgccg taataatatg ctgtacacaa
      481 aataaatata atgtttcaac tgacaccatt taacagcaca tatgacagaa cctcattttt
      541 atgtggctct gatgctctgg accgttacat aaaagagcat gtcacacagg atattcgacg
      601 acgcatcgcg gcctgttttg tcgctgtaga taacgatcat catatcatcg gatactatac
      661 gttatcagcg gccagtatat tattaactga cttacctgag catttgagaa aaaagcttcc
      721 gcgatatccc actattccgg ctattcgtat gggacgactg gcagtaagtc tgtcatcaaa
      781 aggaacgggg cttgggggag cacttttagc tgatgcgcta tatcgtgcat acacatctga
      841 aattgcagca tatgcgctta ttgttgatgc aaaagatgac acggcagtcg cattttacaa
      901 acattatggc tttatggcgt tcccggagtc cggaagaact ttattcatgc cattggcatc
      961 attatccaca ataaaataaa cagaataaat cagggaaata cggtgcttgt ggcaacgtca
     1021 gcccacgaag ttaaatgtta acgggcaaca
//
LOCUS       DAZUCC010000503         1044 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZUCC010000503 DAZUCC010000000
VERSION     DAZUCC010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.506"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..295)
                     /locus_tag="ACHY09_005332"
     CDS             complement(<1..295)
                     /locus_tag="ACHY09_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFS3888124.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTVSWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLN"
     gene            complement(350..655)
                     /locus_tag="ACHY09_005333"
     CDS             complement(350..655)
                     /locus_tag="ACHY09_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888125.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            complement(708..>1044)
                     /gene="tssE"
                     /locus_tag="ACHY09_005334"
     CDS             complement(708..>1044)
                     /gene="tssE"
                     /locus_tag="ACHY09_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFS3888126.1"
                     /translation="RILNTRAGSLKHLPDYGLPDMTSILQGMPGTAHQLMRVLSDVLL
                     KYEPRIKRVGVTMQEQTQPGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQF
                     VDNVMTFRG"
BASE COUNT          274 a          283 c          233 g          254 t
ORIGIN      
        1 tattcagcgg ccacatcact gaccagtgat gccgggtcag cgccacaatc agagccagcc
       61 cttcattcag accacttaac cctgctgtat gcatccgggc aatggtgtac caggataccg
      121 tctgtaattc cacgccattg tgttcataca gacgcaggca gagcgtttcc acgtaacgcc
      181 agtccacatc cggacgtgcc gggtgggtca gtttgctgat ttcatcacgc agggcagcat
      241 aatccggtaa tgaccggggg tcaccaccgg ttttcagttt cacttcagaa gtcatcggca
      301 gattcggtgt tgctgtatta aatcatgatt aacagttacg gaaaggacat cagtgtgata
      361 ccggtacccg ggaaaggtat tccaccgcac ggatgattgc cggtgtcaca tcccaccgcc
      421 gcttcagaac cgctaataac tcactgagtg ttctgatata acgttcagtg aacagtcgct
      481 gcccgtccct ggttaaatga aaaccggact gattacaggt aatggtaata tggcatgcat
      541 cattaccaat atatacggct tcctgtggcc tgaatctgac attccagtgc caggaatacg
      601 gagcatgatt cagataaaaa aggcgcatct ggatgtcatt aaataacggg ttcatcattc
      661 agccctcgtt tatgtctgtt aagcacacgg ataaggggaa ccataaatca ccccctgaaa
      721 gtcatcacat tatccacaaa ctgctgttgc ttcagatatc gcagtaacac cctgccctcc
      781 ggcataaaag ttgttccgta acgaaccagt cctgcatctt taagttctgc atcaatgaca
      841 taatgcaact caccaggctg agtctgctcc tgcatcgtca ccccgacacg cttaatacgt
      901 ggctcatact tcagcagaac atctgaaagc acacgcatca gttgatgcgc cgttcccggc
      961 attccctgca gaatgcttgt catatccggc agaccataat ccggaagatg tttaaggctt
     1021 ccagcccggg tattcagaat acgc
//
LOCUS       DAZUCC010000504         1041 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZUCC010000504 DAZUCC010000000
VERSION     DAZUCC010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.507"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..>1041
                     /locus_tag="ACHY09_005335"
     CDS             2..>1041
                     /locus_tag="ACHY09_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFS3888127.1"
                     /translation="MTAGARHTVKAQAGEQYGLVDEVTGLVPDGVEADRSGDDLILRK
                     KEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQDGPVLENFTAGQSGT
                     LSDDDRGGFIWLGGLAFGGGLAAMALAAGGGGGSKHRHENHEADTTAPSAPELTAGDN
                     GSVSVELPGDANKGDTVEITFEDENGDEQKVTLEKGDNGWTSSNPALIPDSQGDTATI
                     APDTVKDNSEVTAVAKDPAGNESTPVTVTSKTDGVADAPVLTIPEVADSVANAAELKD
                     GLQAEVKLPAGTVEGAVITLTVTHPDQSTENVTHNVTGDEVTAGKVSMDIPEDAVVDG
                     QNSVSVSLTQGS"
BASE COUNT          284 a          207 c          321 g          229 t
ORIGIN      
        1 actgacagcc ggagcccgtc atactgtaaa ggcacaggcc ggggaacagt atggactggt
       61 tgatgaggtg acaggtctgg tcccggatgg tgtggaagcg gaccgttctg gtgatgattt
      121 aattctgcgt aaaaaggaag atgatacaga aatccggatt gagggattct gggaagaatg
      181 ccagccgggt gaaacacagt gtacagcagt atttaatgtt gtgggtgaaa atggtcaggt
      241 aactgaagct gtcctgaccc aggatggccc tgtgctggag aatttcactg caggacaatc
      301 cgggaccttg tcagatgatg atcgtggtgg ttttatatgg ttgggcggtc tggcatttgg
      361 tggggggctt gctgctatgg ctttggctgc aggtggtggt ggcggcagca agcacagaca
      421 tgaaaaccat gaggcagata caacagcacc gtcagcaccg gaattaacgg ccggagataa
      481 tggttcagtt tctgttgaac tgccgggtga tgcaaataaa ggcgatactg ttgaaatcac
      541 ctttgaagat gaaaatggtg atgaacagaa agtcacactg gagaaagggg ataacggctg
      601 gacatccagc aatcctgcac tgatcccgga cagccagggt gatacagcca caatcgcccc
      661 agacactgtt aaagataaca gtgaagtcac agcagttgct aaagatccgg ctggtaatga
      721 aagtactccg gtcactgtga cgtcaaaaac tgatggtgtg gcagatgctc cggtcctgac
      781 cattccagaa gtggctgata gtgtggccaa tgcggctgag ctgaaagatg gtttacaggc
      841 agaagtcaag ttgccggcag gaacagtgga aggtgcggtc attacactga cagtgactca
      901 tccggatcaa agtaccgaaa atgtgacaca taacgtcacc ggagatgagg ttactgccgg
      961 taaggtatcc atggatattc ctgaggatgc tgttgttgac ggtcagaaca gtgtgagtgt
     1021 cagcctgacc cagggcagta a
//
LOCUS       DAZUCC010000505         1039 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZUCC010000505 DAZUCC010000000
VERSION     DAZUCC010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.508"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1039)
                     /gene="ompC"
                     /locus_tag="ACHY09_005336"
     CDS             complement(<1..>1039)
                     /gene="ompC"
                     /locus_tag="ACHY09_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFS3888128.1"
                     /translation="ASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDT
                     TYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSID
                     YGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFA
                     AQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQVIYGNNSLNASG
                     QNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQYQFDFGLRP
                     SVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKSDFTKASGV
                     ATDDIVAVGMVY"
BASE COUNT          284 a          240 c          222 g          293 t
ORIGIN      
        1 ggtaaaccat acctacagca acgatatcat cggtagcaac gccagatgct ttcgtgaaat
       61 cgctcttatc aatcaggttg attttgtaat caacaaaagt ggacatattt ttgttgaagt
      121 aataggttgc acctacatca atatattcaa ccaggtcctg atcaccccac gcacccaagt
      181 cttttccttt agattgcagg taagcaacgg acggacgcag accgaagtcg aactgatatt
      241 gtgcaactac ttcgaagttt tgtgctttgt tggcaatatg gttattacca aaaacagtca
      301 tgttctgggt ttcagaatag gtggtagcca gatagatgtt gttcgcatca tatttcagac
      361 cagctgccca tacttcagca ttttgaccag atgcattcag gctgttgtta ccgtagataa
      421 cctgattatt agtgcggtca gatttagcat aggttgcacc tacaccgaat ccttcatact
      481 cataagtagt ggagaaaccg aaaccatcac cattagcttc agttacgtca gtgcggtcat
      541 ttttaccctg atactgagca gcaaagttca gaccatcaac cagaccaaag aagtcgttgt
      601 tacgataagt tgcaacacca gtggtgcgac cagtcatgaa cacatctgtt tgggtccagg
      661 tatcgccacc gaattctggc agaacgtcag tccacgcacc gatgtcatat gctacaccgt
      721 agttacggcc gtaatcgatt gagccgtagt ccccgaattt caggcctgca aatgcaagac
      781 gggttttgtc tttggaggag ccttgagatt cagcgcggtt gcctttgaat tcatattccc
      841 actgaccgaa accagtcagt tgatcgttga tttgggtttc acctttgaag ccaagacggg
      901 cataagtagt atcaccatca tctgcatcat tagaggagaa gtagtgcttg gcattaactt
      961 tcccatatag atccagcttg ttactgtctt tattataaat ttcagctgcc tgagcagaca
     1021 tcgccatcag tactgatgc
//
LOCUS       DAZUCC010000506         1036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZUCC010000506 DAZUCC010000000
VERSION     DAZUCC010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.509"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..587)
                     /locus_tag="ACHY09_005337"
     CDS             complement(<1..587)
                     /locus_tag="ACHY09_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFS3888129.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITGLLTAEK
                     ARGMKGDEINAWRNAMEAANYMFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVR"
     gene            complement(629..>1036)
                     /locus_tag="ACHY09_005338"
     CDS             complement(629..>1036)
                     /locus_tag="ACHY09_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3888130.1"
                     /translation="DPGAQGPKGETGATGPAGPRGAQGPKGEKGDPGPRGERGETGPM
                     GPPGASDGKSRVVGIRLGNEQTYRAEESSGFLSISLSIGGVITGLKSRGADGVISVSY
                     RPLQVTFDGSQWRTVSVGEYVSSGASGFEYFPL"
BASE COUNT          199 a          366 c          206 g          265 t
ORIGIN      
        1 cggacgtgga tttccatccc tttggtgaac atcgcctgct ctgccgcttc actcagtgct
       61 ataagctctt ctgctgtcac ctccacatca ttgttttccg catccgtcca gaaaaacgcc
      121 tccggcagtt tccccgcttt cgctgccgcc accgacggct caagacgcgt ctgcgttgac
      181 ttcccgtagt cccatttacg cccattgtgc tcaaacatgt agttcgccgc ttccatcgca
      241 ttacgccagg cgttaatttc atcgcccttc atccctcgcg ctttctccgc ggtcagcagg
      301 cccgtgattt tctccccgtc aaaaccccag cgccccgaga tgtcagcttt gcggttttct
      361 tccgtatccg ccacctccac cacactctgg cccactggcc acagaactga tgccaccctg
      421 ctgatgccgg taatgacccc ggcactgtta taaaccacct tcagcgtctc tgctgaaaat
      481 aacgactgac actcatacca gtcctgacca tcctctgatt tcaggtacat cgcacccggc
      541 acatccggat tttccggcgt gtattgtctg aaattcttta tgtgcatctg taagtcctcc
      601 cggttacatg tctgaaagat gatgcgcttt ataatggaaa atactcaaac cccgaagctc
      661 ctgacgacac atactcaccc acgctgactg tacgccactg agaaccatca aatgtcacct
      721 gaagcggcct gtaagaaacc gatatcacac catctgctcc tctacttttc agaccagtaa
      781 tcacacctcc tatactcagg cttattgaca gaaaaccaga gctctcttct gctctgtaag
      841 tctgttcatt acccagcctg atacccacaa cccggctctt cccgtctgat gccccgggcg
      901 gccccatcgg cccggtttct ccacgctcac ctctgggacc ggggtcgcct ttctcccctt
      961 tcggtccctg cgctccccgc ggaccagcgg ggcctgttgc tcccgtttca cctttcggac
     1021 cctgtgctcc ggggtc
//
LOCUS       DAZUCC010000507         1034 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZUCC010000507 DAZUCC010000000
VERSION     DAZUCC010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.510"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..942
                     /locus_tag="ACHY09_005339"
     CDS             <1..942
                     /locus_tag="ACHY09_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS3888131.1"
                     /translation="QQEGSEDGHGVVFPAELVRMLDRLEGEIRADRVSSESRQWLAQC
                     GLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALISTEGATEWRGEYDEWGNLLNEE
                     NPHHLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLEGGWNLYNYPLNP
                     IIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEM
                     LSDICIYATACGHAGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYV
                     VDVDNPESSTESVGIGAGVDASVMTLACRTWQECWVN"
BASE COUNT          275 a          186 c          307 g          266 t
ORIGIN      
        1 cagcaggaag gaagcgagga cggccacggt gtggtgttcc cggcagaact tgtgaggatg
       61 ctggacaggc tggaggggga aatccgggca gaccgcgtga gcagtgaaag ccgccagtgg
      121 ctggcacagt gcgggctgac ggtggagcgg ctggcggcac agatagagcc ggtttattta
      181 cctgagcgaa aaattcatct ttatcactgt gaccaccggg ggctgccgct ggcgctcatc
      241 agtaccgaag gagcaacgga atggcgtggg gaatatgatg aatggggcaa cctgctgaat
      301 gaggagaacc cgcatcacct gcaccagcca taccgtctgc cggggcagca gcatgatgag
      361 gaatcaggac tgtactataa ccggaaccgg tactatgacc cgctacaggg gaggtatatt
      421 acacaagacc ccattgggct ggagggggga tggaacctgt ataattaccc actgaatccg
      481 ataataagga tggatccttt gggtttgtat aatttatatc aattattata tgatgtttgg
      541 catgatgatt catatggaac atcatcaatt gatattactg gcagtggaga tctaatatca
      601 ttaggtggtc atgcaggact tggcgttgcg tttgctaaaa agaaaggtga aatgttatct
      661 gatatttgta tttatgctac agcatgtgga catgcaggaa ttggtggtgg gataaatgcg
      721 gctatcacat attctgagac caagtcttta cctacatcgg gagtcagtaa ttcagtaggt
      781 gtaacggttg gcggcggagt tggggggcat tttgcgtata cttatgtagt ggatgttgat
      841 aatccagaat catcgacaga atctgttggt atcggtgcag gtgttgacgc ttcagttatg
      901 actctggctt gtagaacgtg gcaagaatgc tgggtcaatt aacaggagga aatatgaaaa
      961 aatatatatt tttagtttta tttttattgt gtggttgttc tccagtggac atagagccag
     1021 ctagacaaga agtt
//
LOCUS       DAZUCC010000508         1033 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZUCC010000508 DAZUCC010000000
VERSION     DAZUCC010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.511"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..386
                     /locus_tag="ACHY09_005340"
     CDS             132..386
                     /locus_tag="ACHY09_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFS3888132.1"
                     /translation="MKITTLSSRELNQDVTRAKRATNYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
     gene            386..802
                     /locus_tag="ACHY09_005341"
     CDS             386..802
                     /locus_tag="ACHY09_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFS3888133.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCSRLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
BASE COUNT          299 a          216 c          223 g          295 t
ORIGIN      
        1 gcatacatta gcattaatat ctgacagcaa caagcggcga agactacccc taatcaagta
       61 catttcttgc attgtactat ttcatgccct acaatgtagt aacgtaccta tatacatcaa
      121 aaggcaacag catgaagatt acaaccctat ccagtcggga acttaatcaa gatgtaactc
      181 gagccaaacg cgccacgaat tatggtccag tatttattac tgatcggggt aaaaccgcac
      241 atgtacttct tagtatcgaa gaataccgaa aattgacgca acaacgacga actctcattg
      301 acattctggc gatgcccgat gctgatgcag ttgaatttga accgcctcgt attacggtta
      361 gtttaaaacc tgcggagttt ttatagtgta tattcttgat actaatgtta tctctgagct
      421 acggaaggct cggacaggac gtatagataa taatgttgca aaatgggcat tgcatacaga
      481 tgcggcagat ttctttattt ctgttatttc cataatggaa cttgaacagg gaatattaca
      541 agttgaacgt cgagatatgc gacaagggat tatgctacgt tcttggatgg atcagcatgt
      601 gttaccagcg ttttctgata agatacttcc tgtagatact gcggttgcat tacgttgttc
      661 tcgtttacat gttcccgaca ggcgcagtga acgagacgca ttgatcgcag caacggcgct
      721 ggtacatggg atgacggttg taaccagaaa tgttggtgat tttgaattaa ccggagtatc
      781 aattattaat ccctgggctt gatatcgctt cccttcttct aggggtccgc ttggaaaacg
      841 gaaattttcc cacgctaaga gaattgagtt gaacgagtgg ttacatccaa gccagctaac
      901 tcactgaacg taaaggctgt acgaaatgtt ccgaccgtcc ggattcctga cagtcatttg
      961 gctaaaaaat agtcttaacg tggtttattt tccgaattcc aggctgaccc cataaaagca
     1021 tccttcagtt cag
//
LOCUS       DAZUCC010000509         1030 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZUCC010000509 DAZUCC010000000
VERSION     DAZUCC010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.512"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1030)
                     /locus_tag="ACHY09_005342"
     CDS             complement(<1..>1030)
                     /locus_tag="ACHY09_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFS3888134.1"
                     /translation="IKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTLAG
                     LSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGTDV
                     SGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQESDI
                     PAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDNPL
                     TRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTDPY
                     PFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVRTL
                     PSSDVNGSS"
BASE COUNT          188 a          356 c          232 g          254 t
ORIGIN      
        1 tactgctgcc gttcacgtct gatgacggca gcgtacgcac tgtcccgtcg gcaaaaacaa
       61 acgtgatgct gttcacctgc ccacggacac aggagagcgt ccagtcacca ctcgcagtac
      121 cggacaccac tgcaccttcc acatccggca gctctattcc gtttgccgtc aggttatcct
      181 tacccaccag gatcttgaac ggatagggat ccgtcacggt tccgttcacc ggaacccgtc
      241 ccagaagcgc tgtcatcgcc cggctgcctg ccagcgtcga gttctccggc agggtataca
      301 ctgcccgggt gctcgccttc tgctgttcat tgctgtatcc cttcacctgc tgttcataag
      361 ccgctttctg acgggtcagt ggattgtcat ccagaaaaga gactggaaaa cgcggtccgc
      421 tcttatcccc caccgacgac gcttcatcct gcacctgatc ggccggtgtc acccacatca
      481 gaccatccgt tcccggtgag gctgccgatc ctgtcacgct ccccagtccg tcaaggccca
      541 gacctgctgg aatatcgctc tcctgtcctc cggcgccggc atcagcactc tcctgctgac
      601 gcttcagttg ctctgtcagt gactgaacct gtgtcattaa cgcgttctgt tcattccgga
      661 actggcgctg cgactgctca taatttttct gcaatcctgc cactgcctca ctgacctgac
      721 cggaaacatc cgtgccccgc ttgcgcagca tttcattttc tttcagcaac ttttcattct
      781 gtttgtccag taccagttgc cgctccctca cctgattcat acgcccaatc agtgtgcgca
      841 gggtatcttc aggtgtgtct ccttccacgc ccagggcctt cagttcatcc ggtgacagtc
      901 cggccagcgt tcctgagctt tccgcagacg agggctcccc ggtatctcct ccacagcttt
      961 ttaatcccgt caggatcccc gccaccacca cacagggcac cacatacttc acgatcgtat
     1021 tggatttaat
//
LOCUS       DAZUCC010000510         1019 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZUCC010000510 DAZUCC010000000
VERSION     DAZUCC010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.513"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..364)
                     /locus_tag="ACHY09_005343"
     CDS             complement(<1..364)
                     /locus_tag="ACHY09_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888135.1"
                     /translation="MYQNRALNTAERLKAKPLPHVKNRVTKPLSAFLASFLLLCRYTC
                     CQIRAQSSLINLRLSFDRSRWYFLTTGAHENARREPSSAPSDMLGIKNPCAAQPALHK
                     IPLCGEYCLIKNPRSSSGK"
BASE COUNT          246 a          232 c          277 g          264 t
ORIGIN      
        1 tttttccgga agaactgcgg ggatttttga ttaaacaata ctcaccgcag agcggaattt
       61 tatgcaacgc cggctgtgcg gcacagggat ttttaatccc cagcatgtca gagggtgcgg
      121 atgacggttc ccgtctggcg ttttcatggg cgccggttgt caaaaaatac cagcgtgatc
      181 ggtcgaagga taagcgcaga ttaatcagtg atgattgagc gcgaatctga caacaagtat
      241 agcgacacag caacaaaaaa gacgccagaa acgcgctcag aggctttgta acgcgatttt
      301 ttacatgagg caatggtttc gctttgaggc gttcagccgt gttcagggcg cgattctggt
      361 acatacctga ctgtaatcat ccctacgaac tactctttgc accagaaacg cgcaggagtg
      421 ttttagcgtg tctccggcgt gatgtcgagt tgtgcggtaa tgagtgagcg aaaaaaatcc
      481 cctggattac agggggaacc catgatcaga ctttgaagtt tatttgttat ttgatggaat
      541 gagcacgaat cccggagatg acgttcaggt ggcaggaaac gcgctcagaa gctttctaac
      601 gcactttttt gcatctggca atgggtttca tatgttttgg tgggttcagg ctgtcagaga
      661 cgttttgagc gcgtttctgg cgcttgctgc tactgccaaa ccatccccat ggccagttga
      721 tgccagaaac gcgcaggaat tttgtagcgc gtctccggtc cctgatcaat gaattccctg
      781 tcattaacat gcgtttaccc ggaaacgcgc cctgatgttc tatcgtatcg cgggaacggt
      841 gtcgagggtg tcggggcaac gccctgacca ggtggtaatc gtatagccgt gcgtgcgcgg
      901 ttatacgatt acacatcctg tcccggaatc aggattgttt ttcgcgcaca actgcggcaa
      961 attcatcccg ttgcggttgc aacgatgggt atatcaaaac tacgaaaatt attcaggca
//
LOCUS       DAZUCC010000511         1016 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZUCC010000511 DAZUCC010000000
VERSION     DAZUCC010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.514"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(204..752)
                     /locus_tag="ACHY09_005344"
     CDS             complement(204..752)
                     /locus_tag="ACHY09_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS3888136.1"
                     /translation="MRRYLRFCTPLSGMAVSPSSLLLQIRPQMSVLAECLIRQLITDE
                     PSSVYFRDAVAFSSLALFEEHPDFHALLTGCIRSCTVRVKNIISADLSRPWMLGEVAS
                     LLYISESLLKKKLQEELTTFSELLLKERMQYAAQLLSAGENRISRVAELCGYASASYF
                     ISVFRRYYGITPNQWLPVMQPD"
     gene            792..>1016
                     /locus_tag="ACHY09_005345"
     CDS             792..>1016
                     /locus_tag="ACHY09_005345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888137.1"
                     /translation="MSLKSRAQMPGNQKHCAGRYDRVPVLQTKHCLFESHNDSAGTGH
                     RISGKYADLLKRQTCPVYKIPCHRVLKKTAP"
BASE COUNT          310 a          262 c          234 g          210 t
ORIGIN      
        1 taaattgttg taccggcaga tgcagccacc ggtagtatca ggaaggaata cagtaacagt
       61 cggtacatga tgcgactccg tcagacaact ggtatctgac agcgtgcgca aaggcaaagg
      121 gacaatcagc aataaattca aaaccggatg ataaaaatta agcctcttcc tctgaataac
      181 gcacactctg catctgaatc aaattaatcc ggttgcatca caggaagcca ctgattcggg
      241 gtaataccat aataacgcct gaacacagaa ataaaataag aagcactggc atagccacac
      301 aactctgcca cccgggaaat acggttttct cccgcagaca aaagctgagc ggcatactgc
      361 attctctctt tcagaagaag ctcactgaag gtcgtcagct cttcctgaag ctttttcttc
      421 agtaaactct cacttatata taacagactg gccacttcac ccagcatcca tggacgggac
      481 aaatcagcac tgataatatt cttcacccgg acagtgcagc tccggataca ccccgtgagc
      541 agggcgtgga aatccggatg ttcctcaaac agcgccagac tggaaaaagc caccgcatcc
      601 ctgaagtaca ccgacgatgg ttcatctgta ataagttgtc tgatcagaca ttccgccagc
      661 acggacatct gtggccggat ctgtaaaagc aaagacgacg ggctgactgc cataccggat
      721 aatggcgtgc agaaccgcag ataccgcctc accacctcct cactgaggtc agcgataagg
      781 aaacgtccgg tatgagcctg aaaagcaggg cgcagatgcc cggcaatcag aagcattgtg
      841 ccggcaggta tgacagagtt cctgtgctcc agacaaagca ttgtctgttt gagagccata
      901 atgacagcgc aggtacaggg catcggatat ccggtaaata tgctgattta ttaaagagac
      961 agacatgccc cgtatataaa attccgtgtc accgggtgct gaaaaaaact gcaccg
//
LOCUS       DAZUCC010000512         1013 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZUCC010000512 DAZUCC010000000
VERSION     DAZUCC010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.515"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..>1013
                     /locus_tag="ACHY09_005346"
     CDS             6..>1013
                     /locus_tag="ACHY09_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFS3888138.1"
                     /translation="MSQVSAQADTNKLTVEVDETILGHDVYGDDWKSIEIGTTKFTAK
                     DESKGINFDHKSPVAEGRNSIAIGTSAEAGGDISTKEGLYTGESIAIGAKTKATKEQS
                     IAIGGDTKATGWGAIVIGGDDLTPLHGKTYGRGKIENGYLASEAQGDGSIALGSRTFA
                     KGELSVSLGSVSSAEGMASNAIGATAEAKGDMATAVGFNALANAGNALAVGSQSSANK
                     NDALAVGSEANAAQNETVALGHGATVTVAKGNVSIGADSRDKQAQKVTEAKVRTKRGA
                     EVTYSGFAGNNAASVVSVGDAGKERQIVNVGAGAISDTSTDAINGSQLYAIAKTLKDD
                     LD"
BASE COUNT          293 a          185 c          302 g          233 t
ORIGIN      
        1 catccatatc acaggtcagt gctcaggcag atacaaataa gttaactgtt gaagttgatg
       61 aaaccatcct tggccatgat gtctatggcg atgactggaa gagcattgaa ataggtacaa
      121 cgaaatttac agcaaaagat gaatcaaagg gaattaattt cgaccataaa tcaccggtgg
      181 ctgaaggacg taactctatc gcgattggta ccagtgcaga ggctggaggt gacattagca
      241 cgaaggaagg tttgtacaca ggcgagtcta ttgcgatagg agcgaaaacg aaagcaacta
      301 aagaacagtc aattgcaata ggtggcgata ccaaagctac cggatggggg gctattgtta
      361 ttggtgggga tgatttaact cctttgcatg gtaagactta tggtcgggga aaaatagaga
      421 atggctatct tgcatcggaa gcgcaggggg atggcagtat tgcgctgggc agcagaactt
      481 ttgctaaagg cgaattgtca gtatcgcttg gttcggtgag ttctgctgag gggatggcct
      541 cgaatgcaat tggagccacg gcggaagcta aaggggatat ggcgactgcg gttggtttta
      601 atgcgctggc taatgccggt aatgctctgg ccgttggtag tcagtcctct gcaaataaaa
      661 acgatgctct tgctgttggt tcagaggcaa atgccgctca gaatgaaacg gttgccctgg
      721 ggcatggagc aaccgttact gtcgcaaagg ggaacgtgtc catcggcgct gactccaggg
      781 ataagcaggc acaaaaagta acagaggcta aggtcagaac taaaagaggc gcggaagtca
      841 catatagtgg ctttgccgga aataacgcag catcagtagt atctgttggt gatgcgggta
      901 aagagcgtca gattgttaac gtgggggcgg gtgcgatttc tgacacaagt acagatgcca
      961 ttaacggtag tcagttatat gcgatagcga agacactgaa agatgacctt gat
//
LOCUS       DAZUCC010000513         1001 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZUCC010000513 DAZUCC010000000
VERSION     DAZUCC010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.516"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..594
                     /locus_tag="ACHY09_005347"
     CDS             <1..594
                     /locus_tag="ACHY09_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS3888139.1"
                     /translation="YYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMG
                     LASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRAL
                     SPYDYTLYCRKWVKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEP
                     YLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            591..>1001
                     /locus_tag="ACHY09_005348"
     CDS             591..>1001
                     /locus_tag="ACHY09_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888140.1"
                     /translation="MKRILFFLLMIFVSFGVIADCETQAKDQDCFTIFTKGTIFSAFP
                     VLNNKTIWRWYQNEDIGEYYWQTELGICKNNKFTPSGARLLIRVGSLRLNENHATKGT
                     LQELLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPA"
BASE COUNT          334 a          198 c          216 g          253 t
ORIGIN      
        1 tattacaacc gccaccgcta ttatgacccg ctgcaggggc ggtatatcac tcaggatcct
       61 attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt tcagtatata
      121 gattcaatgg gactggcatc aaaatatgga cacttaaata atggcggata tggagcgaga
      181 cccaacaaac cgcctacgcc cgatccaagt aaattgccag acatagcgaa acaattaaga
      241 ctgccatatc ctattgacca ggccagtagt gcgcctaatg ttttcaaaac attcttcaga
      301 gcattaagcc cttacgacta cacactgtat tgcaggaagt gggtaaaacc aaatctgact
      361 tgtacgccac aggatgatcc ccagtatcca gggatggata caaagacagc aagtgattac
      421 ctgccacaga caaattggcc aacaactcaa ttaccaccag gatatacttg tgcagaaccc
      481 tatttattcc cagacattaa taaacccgat gggccagcaa cagcagggat agatgatttg
      541 ggtgaaattt tagctaaaat gaaacagaga acatcgagag gaataagaaa atgaaaagaa
      601 ttttgttctt tttgctgatg atatttgtta gttttggtgt tatagctgat tgcgaaacac
      661 aagctaaaga tcaagattgc tttaccattt tcactaaggg tactattttt tccgcgtttc
      721 ctgtcttaaa taataaaacc atatggagat ggtatcagaa tgaggacata ggtgagtatt
      781 attggcaaac agaacttggt atatgtaaaa ataacaaatt cacaccaagc ggggcgagac
      841 tcttaattcg cgtcggatca ctacgcctta atgaaaatca tgcaaccaaa ggaacgttgc
      901 aggagttgct aaatacagca gaaaaaacgg cgtttctcgg cgatcggttt agaagttata
      961 taagagcggg tatctatcaa aaaaagagca gtgatccagc t
//
LOCUS       DAZUCC010000514          999 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZUCC010000514 DAZUCC010000000
VERSION     DAZUCC010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.517"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(96..473)
                     /locus_tag="ACHY09_005349"
     CDS             complement(96..473)
                     /locus_tag="ACHY09_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS3888141.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            complement(478..>999)
                     /locus_tag="ACHY09_005350"
     CDS             complement(478..>999)
                     /locus_tag="ACHY09_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFS3888142.1"
                     /translation="ERDQYLGRDILFVVDEAHIVTVNPLLSPYMTKVVKMWRKLGAWL
                     WLATQNLKDYPDVSEKMLNMAEWWVCLTMPPEEVNNIARFKSLTEEQKAVLLSASKLS
                     GCYTEGVVLAKRTEALFRVVPPSLYLALGMTEKEEKAERRALMKVHQCSELEAAFHVA
                     RKLDGLRGLAGRE"
BASE COUNT          231 a          352 c          211 g          205 t
ORIGIN      
        1 ccacagctcc cgccagaaac agcacaggtt cactggcaat ttgatctgac agcgatggat
       61 acagtggtcc cggaaaatcg atatcgctcc tcatatcacc cctctcccag ccacaacagc
      121 agatagaaca aatccgccgc atcagtcact gatatatacc agcggggatg aatatactcc
      181 tcctccgacg gccagctctc atgcccactc acagtaagca gaatatgcac atactgaccc
      241 tgctgacctt ccagcatcac cgccacactg ctgttcagac ccaccctctg tggctggcaa
      301 cggatacact ccacccgccc cagttgcgcc gccagatgcc cgcgaagacg ttcaagatta
      361 tcccgtgtgg tgcaacttat ccccggcgca ccctgtggcg gcagaacaaa cacacttccg
      421 gcggggtgaa cagcagacac cacttccggt tccggatact tcagacacag catagtctca
      481 ctccctgcca gccagaccac gtagcccgtc cagctttcgt gccacatgaa acgctgcttc
      541 cagttcgctg cactggtgca ccttcatcag ggcgcggcgt tcagccttct cttctttctc
      601 cgtcatcccc agagccagat acagactcgg tggcacaacc cggaacaacg cttctgtgcg
      661 cttcgccagt accaccccct ccgtataaca gccggacagt ttactggctg acagcagtac
      721 cgccttctgc tcctccgtca gggatttaaa acgggcaata ttgttcactt cctccggcgg
      781 catggtcagg cacacccacc actctgccat attcagcatc ttctccgaga catccggata
      841 atccttcagg ttctgcgtgg ccagccacag ccaggccccc agcttgcgcc acattttcac
      901 cactttggtc atatacgggg acaaaagcgg gtttaccgtc acgatatgcg cctcatccac
      961 cacaaacaga atatcccgtc ccagatactg atcgcgctc
//
LOCUS       DAZUCC010000515          993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZUCC010000515 DAZUCC010000000
VERSION     DAZUCC010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.518"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            186..>993
                     /locus_tag="ACHY09_005351"
     CDS             186..>993
                     /locus_tag="ACHY09_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888143.1"
                     /translation="MSIDFTPCMINTYHGDIYSRTTDTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYTSDQYDLAQYQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAEYRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFITEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNV
                     TGQDLSGFVLTLSRFYFEDLLNINFTDANLGDTIFSHKEHPTPKLYKDEQY"
BASE COUNT          376 a          191 c          155 g          271 t
ORIGIN      
        1 gatactatca ctgccctttt tttatacaca aatattaccg ggtggaaatg aaagggaagt
       61 taaaggtaat tatatcagga agatatgacg cattgtgttt atttccgcgt agaagaaaat
      121 ccattcctgc tctaattctc cgatttacaa aaatttagcg aaatagatga acaataagga
      181 atgttatgtc aattgacttt accccatgta tgataaatac atatcacggc gatatttata
      241 gccgcacaac agataccgac aatgttaaaa caccagacac accgaagtgg ccttgcgata
      301 attgggaaga acaacaaccc attaattcca cattctctgg agaaggatat acctctgatc
      361 aatatgattt agcgcaatac caacttcaac aaattaatgc gtgccacacg aacactacat
      421 atacaaatgc agactactca aaagttgtgg ctcaacttgt aagtcttatt actaacattg
      481 aaacgataag ttcgacacaa cttacgcaac agacacaatc aatacttaat cagattaata
      541 acattagata cgaaaaaaac aaaagtgctg aatatcgaat cattgttgtc gctaatccta
      601 aacccgacaa ggcaatcata actaaaatta gcgtagaaga gggaatacca gtaagattca
      661 gtgtccaaac catgttttca gatactaatt ttattactga acaacgagct gacttaccga
      721 ctaatataaa agatattcaa agcctgtatc aaaaaatgac gaagttatat attgaacaca
      781 gtgaaaataa aaacaggatg aaagtctttg ctggaaccaa ctttattgat ttcaatgtga
      841 caggacagga cctttccggg ttcgttctaa ccttgtcaag gttttatttc gaagatctac
      901 ttaatataaa tttcactgac gcaaatttag gtgacactat tttttcacat aaagaacacc
      961 ctaccccaaa attatataaa gacgaacaat atc
//
LOCUS       DAZUCC010000516          985 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZUCC010000516 DAZUCC010000000
VERSION     DAZUCC010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.519"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..189)
                     /locus_tag="ACHY09_005352"
     CDS             complement(<1..189)
                     /locus_tag="ACHY09_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HFS3888144.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTI"
     gene            complement(235..>985)
                     /locus_tag="ACHY09_005353"
     CDS             complement(235..>985)
                     /locus_tag="ACHY09_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3888145.1"
                     /translation="NSLFENTDDGIIYTGIVPLITVVEPVVSNQHSVLIIPGGGYRRI
                     VYEREGLDVGRWIAGQGYTAFILSYRMPSVNGYYSFADAKNALNFISYWAGMSERTFK
                     SVGCMGFSAGGHLAGYITHLHYEEETSVRPDWMALIYPVISLDRSIAHQGCRKRLLDA
                     GESEYSASLENSVTEYMPPVFIVHATDDKDVPVIHSLRYFNSIRDKGHHAEAHFFDKG
                     GHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH"
BASE COUNT          311 a          241 c          157 g          276 t
ORIGIN      
        1 aattgtattc caggtatttg actcaaaact tgcccaccaa ccattattcc acccctgact
       61 aacctgaatc aacgtttcat aagcgtgact ggcgcttctg tatcctccac gtagattaag
      121 agttaatgcc tgagagcaca aaggtaaacc aagaagaatc acagttaata gtggataata
      181 ttttttcata aatgcctcat cattaaatat atttatcact tgtaatctga tttctcaatg
      241 aaacttatgt tccgcaagcc attccagaag caggctttgc catttttcta tcggatatcc
      301 attttttccg gatacaccaa atccatgccc tcccttatca aaaaaatgag cttctgcatg
      361 atgtccctta tcccggatag agttaaaata tctcagagag tgaataactg gaacatcctt
      421 atcatctgtt gcatgcacaa taaagactgg tggcatatac tctgtaacac tattttcaag
      481 actggctgaa tattcacttt cacctgcatc cagaagacgt ttccggcatc cctgatgagc
      541 aatactcctg tcaagagata taactggata tatcagagcc atccagtcag gacgcacaga
      601 agtctcttcc tcataatgga gatgagtaat atatccagca agatgaccac ctgccgaaaa
      661 ccccatacat ccaacactct taaatgttct ctccgacatt ccggcccagt aagaaataaa
      721 gttcagggca ttcttcgcat ccgcaaagga ataataacca ttaacggagg gcattctgta
      781 agaaagaata aatgccgtat atccctgacc tgctatccat ctgcctacat caagtccctc
      841 tctttcataa acaatcctcc tgtatccacc tccaggtata atcagcacac tatgttgatt
      901 agatacaaca ggctcaacaa ctgtaataag gggcacaata cctgtatata ttatgccatc
      961 gtcagtattc tcaaatagcg aattg
//
LOCUS       DAZUCC010000517          982 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZUCC010000517 DAZUCC010000000
VERSION     DAZUCC010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.520"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(144..407)
                     /locus_tag="ACHY09_005354"
     CDS             complement(144..407)
                     /locus_tag="ACHY09_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS3888146.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
     gene            597..836
                     /locus_tag="ACHY09_005355"
     CDS             597..836
                     /locus_tag="ACHY09_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888147.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPDFTPAQDKPQ
                     NMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
BASE COUNT          286 a          233 c          188 g          275 t
ORIGIN      
        1 tggcatccac atagtgcgct gggttatcgc tctccacggg aatatctgcg gcggcgggca
       61 gtaatgggtt aagtgatacc agatgtctgg aaatatagga gcaaatccac cacgcatgga
      121 gcctgttaag actctcactc tcatcattct cccttctgag tcccaacgaa acgaccaaaa
      181 cgctgagtaa agcgggcaac attgccttct gatgccaccg ttctgagttt accggtatag
      241 aacggatgcg atttggagga aacgtcaatc gtcacatacg gatacgtcac accatccagt
      301 tcaatctcgc gatctgtttt gatggtagaa cctattttaa agtactcatc cacactcgtg
      361 tcatggaaca ctactgtgcg gtattcagga tggatatttg gcttcatctc tcttcactca
      421 tgttataaca taacaataag atacattctc agctagatca tttcaacaga atccccgagc
      481 ccaagggtaa tgtggcatcg cagcctctca tcaaatggaa gacatgtgat tgcacattta
      541 tcatacaaga cgtatgataa cactgtgtta tttatcctgc tgctgagggt atagatatga
      601 ataacgaatc tgaaaaagca atgtggcgtt gtaagcctgt ggataatcca tggaccttca
      661 cttccctgct gaaaaaagca gtctgtgccg ttttaccaga tttcacacct gctcaagata
      721 aaccccaaaa tatgttttca ctaagtaatg aaaaagatac tgattattca gtaccaatga
      781 gaccctttgc attatatgac ccgtggaaac atcaccatga caatgctgat ttctaagaaa
      841 agcaagggtt tccccaatta tcccagctaa ggaaacccta agaattttac ttattagcta
      901 aatctccact ttttgctgct gccttagata cattacttgt tcctttgtta attgcatccc
      961 ccataaccgt tccgacactt ac
//
LOCUS       DAZUCC010000518          980 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZUCC010000518 DAZUCC010000000
VERSION     DAZUCC010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.521"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..573
                     /locus_tag="ACHY09_005356"
     CDS             <1..573
                     /locus_tag="ACHY09_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS3888148.1"
                     /translation="MLRLRRAGQITGQHVPEIILLNSHDGTSSYQMLPGYFRAICTNG
                     LVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQ
                     AALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTHAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            628..>980
                     /locus_tag="ACHY09_005357"
     CDS             628..>980
                     /locus_tag="ACHY09_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS3888149.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQSQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIV"
BASE COUNT          226 a          267 c          269 g          218 t
ORIGIN      
        1 atgctgcgtc tgcggcgggc cggacagata accggtcagc atgtgcctga aattattctg
       61 ctcaactccc atgacggtac ctccagctac cagatgttac ccggatattt tcgtgccatt
      121 tgtaccaatg gcctggtctg cggtcagtcg ctgggagaag tccgggtgcc acaccgggga
      181 aacgtggtgg acagggtcat agaaggcgct tacgaagtgg tgggcgtgtt tgaccggatt
      241 gaggaaaagc gtgatgccat gcagtcgctg gtcctgccgc caccggcacg ccaggcgctg
      301 gcacaggcgg cactgactta ccgttatggt gacgaacatc agcccgtcac caccgccgac
      361 attctgacgc cacgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc
      421 attcaggaga atatgctgaa aggtggaatt tcaggtcgca gcgctaaagg aaaacgtatc
      481 catacccatg ccattcacag cattgacact gacattaagc tcaaccgcgc attgtgggtg
      541 atggcggaaa cgctgctgga gagcctgcgc tgattcagcc attaccctga aaaccacgca
      601 tttatttgtc ctttaaggag tcatttcatg aaaaccattt cacataattc caccacatct
      661 tctgtttccg taaccgctgc atcagggaat gaccagtctc agctggttgc cactcccgtc
      721 cctgatgaac agcgcatcag cttctggccg cagcattttg gcctcattcc acagtgggtg
      781 accctggagc cccgtatctt cggctggatg gaccgtcttt gcgaagacta ctgcggtggt
      841 atctggaatc tgtacacact gaacaacggt ggcgcattta tggcccccga accagatgac
      901 gatgatggcg aaaactgggt actgttcaat gccatgaacg gtaaccgcgc tgaaatgagc
      961 ccggaagccg ccggcattgt
//
LOCUS       DAZUCC010000519          975 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZUCC010000519 DAZUCC010000000
VERSION     DAZUCC010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.522"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(111..743)
                     /locus_tag="ACHY09_005358"
     CDS             complement(111..743)
                     /locus_tag="ACHY09_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFS3888150.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          245 a          223 c          195 g          312 t
ORIGIN      
        1 caccgcgagc gccaaataat tttatggcca caacctgtcg tattgtggcc ataaaaaatt
       61 gtcggctgga gggagtaaaa tcctttacct caatatattg cctgtcagct ttagttcaca
      121 aacagtgctt cgctatcgac gatgattcca tcttccgcac ctttaagcgc caggtttgcc
      181 tcgtagtatt gctttttatc aagcagtttc tgcgcatcag caagtgcatt acgagtctgt
      241 ttgagcggca tcagatattg gttttccatt acaccaacac cggccaggcg aagctcttcc
      301 attgcgccct ttttatcccc tttcgccatt ttttcgttgg caatttttat cgcggcttct
      361 ttttcgggtg tcgctacata actctcagaa attccgacag atgcattaat gacaatatat
      421 tggtcatcat tgagattggt ctttttacct ggcttagcga actttgccca ttcggtactg
      481 tcatctgaca acaaagctga agcttcattg gtcagttctt ttgccttttc cggatcgcca
      541 tgaaacagag ccaggcgcgc tacctgcacg tcacgcatcg catataaacc ttgttcagag
      601 atacgctctg ctgctgccgt tttctgggtt tgctgaactt gctgagtttg ctgttgtgcc
      661 gctaccggtg cattatcagc agcccataca gttgaagatg ccagaattgc tgtgaccatc
      721 gtagccataa ttaaacgttt catacttacc tcattcttta acagttttag aaaacgccgc
      781 catcatattg ggtgagcatg aaatgagaaa tcagagagca acggtcattt tgaaagggtt
      841 atcttacagt tgtagttatt atcgcaagaa gtattttagt cttacaattg taggaatatg
      901 cttcctgtca cagagaacta ttcttatata ccacaaagag atgtcattta ttatatcgat
      961 gtaaatactg attaa
//
LOCUS       DAZUCC010000520          971 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZUCC010000520 DAZUCC010000000
VERSION     DAZUCC010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.523"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            89..>971
                     /gene="tdcA"
                     /locus_tag="ACHY09_005359"
     CDS             89..>971
                     /gene="tdcA"
                     /locus_tag="ACHY09_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFS3888151.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELA"
BASE COUNT          265 a          188 c          223 g          295 t
ORIGIN      
        1 aagttaattt gtgagtggtc gcacatatcc tgttcatttc attttgatac acttcatgcc
       61 gtcaatgagg taattaacgt aggtcgttat gagcactatt cttcttccga aaacgcagca
      121 cctggtagtc tttcaggaag ttattagaag tggttctatc ggctcggctg caaaagagtt
      181 agggttaact caaccggccg tcagtaaaat cattaacgat attgaagatt attttggtgt
      241 ggagttagtg gtgcggaaaa ataccggtgt aacattaaca cctgccggtc aattgttact
      301 ctcccgttcc gaatccatta cccgtgaaat gaaaaatatg gttaatgaga taagcggtat
      361 gtcttctgag gcggtggtgg aagtctcatt tggttttcct tcattgattg gttttacttt
      421 tatgtcaggg atgatcaaca agttcaaaga ggtgttcccg aaagcgcagg tttctatgta
      481 tgaagcgcaa ctgtcttcgt tcttaccggc aatccgcgac ggtcgactgg attttgcgat
      541 tggtacgtta agcgcagaaa tgaagcttca ggatttacat gttgagccgc tgttcgagtc
      601 cgagtttgtg ctggtagcca gtaagtcccg aacatgcacc ggcaccacca cgctggagtc
      661 gttgaagaac gaacagtggg tgttgccaca aaccaatatg gggtactaca gcgaactact
      721 tactacgtta caaagaaatg gcatcagtat tgaaaacatc gttaaaaccg actcagtcgt
      781 gacaatttat aatcttgttc tcaatgctga tttcttaact gtaattcctt gtgatatgac
      841 atcacctttt ggttctaatc aatttattac tattccggtt gaagaaacat tacctgtggc
      901 acaatatgcc gcggtatggt cgaaaaatta tcgtattaaa aaagcagcat cggttttggt
      961 ggaattagcc a
//
LOCUS       DAZUCC010000521          970 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZUCC010000521 DAZUCC010000000
VERSION     DAZUCC010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.524"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..>970)
                     /locus_tag="ACHY09_005360"
     CDS             complement(89..>970)
                     /locus_tag="ACHY09_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3888152.1"
                     /translation="GIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYVNLDPVIQPGQN
                     LVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVTYPFPLTTNT
                     NVLDIGDKTPMPLPLKLYITPVGVAGGVVIKAGEVIARIHMYKIATLGSGNPRNFTWN
                     IISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSA
                     RQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNK
                     VSAGTVQSVIGVTFIYE"
BASE COUNT          271 a          222 c          201 g          276 t
ORIGIN      
        1 cttacaggtg taggataata gccttcacaa aataattatc cgcgcaattt tcgcgaatgg
       61 agcgcataga ctaaggtcaa aatcacaatt attcatagat aaaagtgaca ccaatgactg
      121 actgaactgt tccggctgaa actttgtttc ctgtttgtcc ataggttgcg cttaaaccca
      181 gaggtacttt gcttttattt actgtcccca gggagacgtt ttctcccgta gccagtattt
      241 ttccattacg catcagagag acacccactc ccgatgcttt tgttgcatct ggtgctgtgt
      301 tagcaaacac ttgtctggca ctgtcagtgg tagcacccga aagataaaat gagagctttt
      361 gttcacttga gcaatatacg ccaaggggta tttctgcgct tccaggaaag tccgggagat
      421 tcacgttgac attacgacta tcaacggtgc agccgcctgt tggcataaca acactgttat
      481 ttgaaataat attccacgta aaattacgcg gatttccgct tcctaacgtg gcaattttat
      541 acatatgaat acgggcaata acttcccctg cttttatgac aactccaccg gccactccga
      601 ctggggtaat atagagtttt aacggtaatg gcatcggtgt cttatcacca atatccaata
      661 cgttggtatt tgtcgtcaac gggaaggggt aggtcacatt attccagtac agtgatcctt
      721 tatatgactg tagggagcct gcgaaagctg atccttgtac caggtttata tgatcagtgt
      781 cgtaccagcc gccgtaatca ttccagcaac ttatatgctg agacaagtct acaaccagat
      841 tctggcccgg ttgtattaca ggatcgagat taacataaac agaggttgta cctgctccaa
      901 tacttgaccc gccatcgaca ttacaactga aggcaaaaac tttgcctgcc attaatagaa
      961 gataaatacc
//
LOCUS       DAZUCC010000522          968 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZUCC010000522 DAZUCC010000000
VERSION     DAZUCC010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.525"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..586)
                     /locus_tag="ACHY09_005361"
     CDS             complement(<1..586)
                     /locus_tag="ACHY09_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFS3888153.1"
                     /translation="MNVTTQGRRAIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTT
                     YRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNAASQLVTQRAGF
                     FPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDYGRETRREYRLDRNGQVTAVTASG
                     TGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETD"
     gene            complement(617..778)
                     /locus_tag="ACHY09_005362"
                     /pseudo
     CDS             complement(617..778)
                     /locus_tag="ACHY09_005362"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
BASE COUNT          168 a          297 c          260 g          243 t
ORIGIN      
        1 ggtcagtctc ttcacttatc cggtgcctgc ccgcagactg cgccttcagg tagccacaac
       61 tgtcatagcc ataggactca tcgccttcgc catagcccag cccggtgcct gaggctgtca
      121 ccgccgtgac ctgaccgttc cggtccagcc ggtattcccg ccgtgtttcc cgtccgtaat
      181 cttcccggct ggtgacagcc gacacattgc ccgcgctgtc gtaacggtac tcccgtacca
      241 gtgaggggag cagcccgccc catgtggttt cctccgggaa aaatccggcg cgctgggtga
      301 caagctggct ggctgcattc cagccctgct cacaggcaaa accccggtta ctgtgccgga
      361 gcacttcacg tcctgcctca tcacggctga acgtcagcgt ctcatccccc ggcaggataa
      421 cctccgtcag ttctccggcg gcgttgtagc ggtaggtggt tttcagcagg gattcaccgg
      481 gatgtctgtc gcgttcatct tccggtggca ggatacagcg gatgacggtg tgttcatcgg
      541 ggtagtggta ttcaatggcc cgtcggccct gagtggtgac attcacccgc ccgcgttcgt
      601 catagccggt gcgctgtgat gtgccgtccg ggctgaagat acccgtcagc ctgccggatt
      661 tgtcatacgc atactgcgtt ttgccttccg gcgtgatgac ttctgccagg cggcctgcgg
      721 cgtcccggct gtagaggatg gtttcctcca gaccgttaac cctggcggta cacaggccat
      781 cggcgtcata gcggtaacgc caggctactc cgttgtaccc agctcataga atttatctag
      841 caatttgtat taagggggtt tcacccacat gttttaataa actcattaac cagatggcct
      901 cttctctcgt tccggagtat attaccttcg attcatttct aataattcga agtgcttctt
      961 ttgtatca
//
LOCUS       DAZUCC010000523          963 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZUCC010000523 DAZUCC010000000
VERSION     DAZUCC010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.526"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>963
                     /locus_tag="ACHY09_005363"
     CDS             <1..>963
                     /locus_tag="ACHY09_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFS3888154.1"
                     /translation="LAKLGFRTEIAEPTSFDYLDGKKQPTGFFRSLINSLYEAANDDT
                     RTSHALVKHNYQRLLDKIDSGSDRYSPMEYWRALHNPDYRDVLQKTIVKHPSDWYFKK
                     DDAIWQPFLNALKKDAPEWKKYSEDFLDKMAWMQDVTTEKLGPSLWHMHPIMFLAFLI
                     QPNEDIRILRLRAFLRMIRIGEGTIQEDGYRTMFTGVKFTDFSKHPNIRHEANGVVST
                     AAGAYQFLYGTWRNLQRRYSFPDFSPSNQDLGCIALIAGRKALDVVMQGKISEAIHLC
                     RIEWASLPGSPHGQPTANKKMIMEKYELYLAEEKEGKTSLHATT"
BASE COUNT          308 a          192 c          212 g          251 t
ORIGIN      
        1 tctggcaaag ctggggttca gaactgaaat cgcagagcct accagctttg attacctgga
       61 tggaaaaaaa cagcccactg gattcttccg cagtcttatc aactcactct atgaggctgc
      121 gaacgatgat acgcgaacca gtcatgcgct ggtgaaacat aattatcagc gcctgctgga
      181 taaaattgat agtggaagcg accgttactc tccaatggaa tactggagag cacttcataa
      241 ccccgactat cgtgatgttc ttcagaaaac aatcgttaaa catcccagcg actggtactt
      301 caaaaaagac gatgcaatct ggcaaccatt tctgaatgcg ctgaaaaaag atgctccgga
      361 atggaagaaa tacagcgaag attttcttga taagatggcc tggatgcagg atgtgaccac
      421 ggagaaactg ggcccttcgt tgtggcatat gcatccaata atgtttttag ccttcctaat
      481 tcaacctaat gaagatatta gaattcttcg tttacgtgca tttctacgaa tgatccgcat
      541 cggtgaaggg acaattcagg aagacggata cagaacgatg tttactggtg taaaatttac
      601 agattttagt aaacacccca atatcaggca tgaagctaat ggtgttgttt cgactgctgc
      661 tggagcttat caatttttat acggaacatg gagaaatttg caacgtagat attcatttcc
      721 agatttcagt ccgagcaatc aagatctcgg ttgtatagcg ctcattgcag gtcgaaaagc
      781 attagatgta gtaatgcagg gtaagattag tgaagccata catttatgtc gtattgagtg
      841 ggccagttta cccggttcac cgcatggtca accaactgca aataaaaaaa tgattatgga
      901 aaaatatgaa ctctacttag ctgaagaaaa agaaggtaaa acaagtttac atgccaccac
      961 aga
//
LOCUS       DAZUCC010000524          948 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZUCC010000524 DAZUCC010000000
VERSION     DAZUCC010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.527"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            154..354
                     /locus_tag="ACHY09_005364"
                     /pseudo
     CDS             154..354
                     /locus_tag="ACHY09_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
     gene            877..>948
                     /gene="ykgR"
                     /locus_tag="ACHY09_005365"
     CDS             877..>948
                     /gene="ykgR"
                     /locus_tag="ACHY09_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFS3888155.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVE"
BASE COUNT          323 a          197 c          163 g          265 t
ORIGIN      
        1 ctatttttac tcttttctcc accataaaat caataaaagc actaacagta attataaata
       61 aaaacaaagc caaaaatctt ggttccaaag ctggtaacaa ctaaccagtt taaatataca
      121 catcaaaatc agataaacat ttgaggtatt gtcatgacaa ataaacacct atcactgttt
      181 tctcctttta tgctgacaga aaaaataaag ccatgtaatc gtatcgttat ggctcctatg
      241 acgacctggt cagctaatcc tgatggaacc atctctgagc aagaactcga atattataaa
      301 cgtcgctcac aaaatattgg tttagtaatt acaggatata cctatgtcac gccaaggaaa
      361 ttcagacatc gaaaggaact ggctggtttc cgttaatgga ttaaatatcg attactctcc
      421 cgagagtaaa actatcaaat caatgagtaa ccgatacagc ctcgcaaatt ctttcagata
      481 attaacccgt cactgcggca cgttctctcg taaggcgtgc ctgcagtgta atccagtatg
      541 tatccggcat agttgacggt ttaatagact caattcctct ccctggttac ctacaacgat
      601 atcttgtata acaccccagg agatacgacg ttacgatgag atgcaatatg ctcctcagcg
      661 ccaatcaacg ccaggactgg gccagtcttg agatcagact cattcccgta actgatttca
      721 ggcgacatat atcaatgacg ttggggcaat aaaagatagc cgatgtaata catctattca
      781 aatttgtgat acatgtcaaa aaattggttg accaaactcg ttatttatat aagggcactt
      841 acgaagtgca ctctttttta aagcgaggaa gtaccaatga aagagaataa agtacagcaa
      901 atcagtcata aactgattaa tatcgttgtt tttgtcgcaa ttgtagaa
//
LOCUS       DAZUCC010000525          936 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZUCC010000525 DAZUCC010000000
VERSION     DAZUCC010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.528"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            833..>936
                     /locus_tag="ACHY09_005366"
     CDS             833..>936
                     /locus_tag="ACHY09_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS3888156.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSA"
BASE COUNT          226 a          217 c          245 g          248 t
ORIGIN      
        1 tcccctgtaa cgaagtttgc gtaacagcat tttgctctac gagtttgcca gcctccccca
       61 gtggctggct ttttttgtat ccgttcaaca ggaatgttac atacctcaca attaagtcag
      121 ttgaaggttg tctgcccgga tgagaatttg ttagaaaatt tctgcatggt gaatccccct
      181 gagcggcggg gcatatcagc gtcacaggtg tttctgtttt acctctatcc tttctgtgcg
      241 ggttcaggtg ctgatactga actcaccggg aggcacccgg caccatgcaa tgaacggtac
      301 atagcgctac tctccagccc ctctccggag gggcttttta atgaggggaa gacactgggc
      361 agtgaaatgt taaatcactc acaattcagg tagttgacgg ttgtctgctt ggctgagaat
      421 ttgttaaaaa aatcctgcat ggtgaatccc cctgtgcgga ggggcgactg gtgaatggta
      481 tgatctcttt gatgatcgta agcgagaata cgcgggtttg gtggcaccag gccgaactca
      541 ccgggaggca cccggcacca tgcagtatac agagattagg catataccag ggcccctcat
      601 agcaggggcc tttttacatg caaaaaaaag ccgccccggg aagagcggtt ggcaagaaga
      661 aaagcaatat gaacaattaa ttaacgctgc gaataatacc ttacagtaat caccctgcgc
      721 aactgtgagg agtgtatttc ttttttgcgg gtggttgtct ttttcccttg tgtttcggga
      781 cttccgttca ctccttatct tattcagtac attatcccgg ccgggaggat tcatggcatt
      841 taaacactat gacgtggtca gggcggcgtc gccgtcagac cttgcggaac gaataactca
      901 aaaactgaag gaagggtggc agccttatgg tagtgc
//
LOCUS       DAZUCC010000526          930 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZUCC010000526 DAZUCC010000000
VERSION     DAZUCC010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.529"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(143..814)
                     /gene="queE"
                     /locus_tag="ACHY09_005367"
     CDS             complement(143..814)
                     /gene="queE"
                     /locus_tag="ACHY09_005367"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFS3888157.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          248 a          226 c          209 g          247 t
ORIGIN      
        1 attaacgcca cagaatttgt agagaatgat aatgattagt ccgggatggg caagatggat
       61 gagttttcag aggcgacaaa caatataaat gagtagaagt ctttacaggt tccacccatt
      121 tataacgctt ataaatgttt aatcaggcaa tatttagata tttatgtgtt tgcatcgaca
      181 aacgccaatt acgcgcaatg caggtttcaa tgcacaaacg tgtggcatca tccttttggc
      241 taatcggctg cagtgcaatg actcgcggtt tatcatcggt cagcgttgcc agtagttcat
      301 ccagtgcttc aatatcacgt acgcgcccca ctggatgctt gatttcgttg gctcgctcca
      361 gtgcctgtga caacacttca tagccgccgc gcatgttcag ctttggtgat acggtaaccc
      421 aggtattcgg tgtgcagcgt acctcatgag taccactggt ttcaatctgg cagctaaaac
      481 cattcttttc gagcagatca gtcagtggta gcaaatcatg aatgcaaggc tcaccacccg
      541 taatcaccac atgccgcgcg gtgtatccct ggcgaccaat gacagccagc aagtcttcac
      601 tgctcgccgc cccccactta tcactctctt tggtcttcgc cagaatgctg aaaagggaga
      661 cttcccgatc ctcaagcttt tcccaggtgt gtttggtgtc gcaccaggca cagccaaccg
      721 ggcatccctg taaacgaata aaaatggcgg ggacgccggt aaagtaaccc tcaccttgca
      781 gggtctggaa catctcgtta atcgggtact gcatagcatt ctctgtgaag tggataattg
      841 ttaattattg cagatcctgc cacaacaatc atgtcttatt aacattctgt tacaggcagg
      901 tttaaagaaa aatgagaaac agatcgttaa
//
LOCUS       DAZUCC010000527          930 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZUCC010000527 DAZUCC010000000
VERSION     DAZUCC010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.530"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            159..443
                     /locus_tag="ACHY09_005368"
     CDS             159..443
                     /locus_tag="ACHY09_005368"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3888158.1"
                     /translation="MEQKTLSAEPRRSFSNNFKLQMVKLALQPRVSVARIARKHDIND
                     NLLFKWLRLSLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPAFYAR"
     gene            complement(660..>930)
                     /locus_tag="ACHY09_005369"
     CDS             complement(660..>930)
                     /locus_tag="ACHY09_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS3888159.1"
                     /translation="GMFTLYLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGA
                     FAQGLLITFLPVFLLPVLGNIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT          279 a          229 c          203 g          219 t
ORIGIN      
        1 gacgtcagta acactttgct tcgctgctgc ctgacggaaa gacgctctga ctccggtaat
       61 ccccgtattt cgtaagcgta aaccgaccgc cgtatgtagc cattaagcca gtattggtaa
      121 cgtaggtgcc caccttttca aactagtgga cgcctgttat ggagcaaaaa acattatccg
      181 cagaaccccg cagatcattt tcaaataatt ttaaacttca aatggttaaa ctggctttac
      241 aaccaagggt ttctgttgcc cgtattgccc ggaagcatga tatcaatgat aatcttctgt
      301 tcaaatggct caggctctcg ctgatcaaag ggcgtatatc acggcgtctt ccggtaagga
      361 actcttctgg cattggcgtt gaattattac ctgtggagat gacgctggat gagcggcctt
      421 accccgcttt ttacgcgagg tgattatgaa gatcaatgac tagccgtcga accgtgtgca
      481 tgaactgctg cactgaaact tctcagctgt aaaataatcc ttacgctacg tacttcccgg
      541 agcgcttacg attaatcgga tgcagtcctg actggaagaa agaaaaatgg gaaaaaataa
      601 gaggaattaa tatcctccac gttcctgaaa tcctggagga tattaatgtc atcaaaatat
      661 tagcgaacga taatccctaa caaaataccc agtgcgccaa agtcagcatc actgaaggtg
      721 gtgttagcaa aaccaatgtt acccagcaca ggcaataaga atactggcag gaaggtaata
      781 agcaagccct gagcgaaggc acccaaaatc gcaccacgac gtccacccgt tgcattaccg
      841 aatacccctg cagctgcacc tacgaagaag tgcggcacca caccaggaat aatcacgatc
      901 atgttcagca gatacaacgt gaacataccg
//
LOCUS       DAZUCC010000528          921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZUCC010000528 DAZUCC010000000
VERSION     DAZUCC010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.531"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..500)
                     /locus_tag="ACHY09_005370"
     CDS             complement(<1..500)
                     /locus_tag="ACHY09_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFS3888160.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSTPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLCEYLMATVYVTVVTLIRV
                     TILVLSV"
     gene            complement(493..>921)
                     /locus_tag="ACHY09_005371"
     CDS             complement(493..>921)
                     /locus_tag="ACHY09_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative coupling factor TraD, PFGI-1 class"
                     /protein_id="HFS3888161.1"
                     /translation="GNFNNMIMLRVRENRTAELLTTQLSQVEIYTKTLVSGHQDTADV
                     TTGQDFTSSTQDRVGTVKVPLLEPADIVTLPKGQAFALLEGGQLWKIRMPLPATDADD
                     ALMPESIAKIAEAMRRNYHTGEGWWNETPSVNMPEGEQNG"
BASE COUNT          210 a          294 c          215 g          202 t
ORIGIN      
        1 acagacaaca ccaggatcgt cacccgtatc agtgtgacca ccgtgacata tactgtggcc
       61 atcaggtact cacacagata atggctcagc cagcgcccca catcctcact gtactgtgac
      121 acactgttac ccaccacggc accggcattt tgctgcttca gccacgacag aaaacctgtt
      181 tccacaaaca gccactgata cgccgttgct atccattctg tcaccgttac cgatggtgtc
      241 gacagcagca gactgcgggt gaattccgaa gacagccacc ccagttctgt cttcatcact
      301 gcctcactgt gtgctgcccc ctgctccggc cagaaaaaag caatcccgat atactcaacc
      361 agcaggctca gcagcaggga ggaaaacagg atcccgacca gcatccacgg ccagccccac
      421 aacagattcc agagcagtcc gtgctgacgt ggcggagagg gttgtgctgc tctgttcacc
      481 tcacgacggg gttcagccat tctgctctcc ttccggcatg ttcacggagg gagtctcatt
      541 ccaccatccc tctccggtgt ggtaattacg ccgcatggcc tctgcaattt ttgcaatact
      601 ctccggcatc agcgcgtcat ctgcatcagt tgcaggcagg ggcatacgga ttttccacaa
      661 ctgtccccct tccagcagag caaaggcctg tcctttcggc agggtcacga tatctgccgg
      721 ctccagcaaa ggcaccttca cagtaccaac acgatcctgg gtactggatg tgaagtcctg
      781 gccggtcgtg acatctgcgg tatcctgatg accggaaacc agcgttttgg tataaatttc
      841 cacctgggac aactgggtcg tcagtaactc ggccgtccgg ttctccctga cccgcagcat
      901 aatcatgtta ttaaagttac c
//
LOCUS       DAZUCC010000529          919 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZUCC010000529 DAZUCC010000000
VERSION     DAZUCC010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.532"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(374..>919)
                     /gene="hdeD"
                     /locus_tag="ACHY09_005372"
     CDS             complement(374..>919)
                     /gene="hdeD"
                     /locus_tag="ACHY09_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFS3888162.1"
                     /translation="LKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILSTVV
                     GALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIAAFI
                     AGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVSTLVG
                     IELIFSAASLFSFASLFVKKQ"
BASE COUNT          317 a          209 c          187 g          206 t
ORIGIN      
        1 ggtttttata tagcgtctgg agacacggat atttatgcaa tgtaatcaac gccagttgca
       61 aaaaaatgaa cccggattac ttatttaacc gcccatagca tcaacgctta cggaactctt
      121 tgttcacttt cccacagcac ttttaaaaca ttaaacctga ccacggaaaa tatcagccat
      181 gataatgttt gaaatggcta atttgccatg gaacgaaaaa aatcagatta aattcagtag
      241 gttgaaataa tcaacagcag gcaattgttt caatgatagt gcgtaattga actgaaacac
      301 tgtatagcta agagagtatt aactggatca tagtcactta atgtgactat gatccaggtg
      361 gaaataggga taattattgc tttttaacga acaaactggc gaagctgaac agactggcgg
      421 cgctaaatat cagttcaatt cccaccagtg tggaaaccag cgttaccgac accatcggcg
      481 tagcaccaag gaatatccag gcaatgacga tatccagcac accaataacg agctgtagcc
      541 aactgccttt cattgaacgc tgacgatacc aactcatcag gcgaataacc cctgccacac
      601 agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc cagctctggt gcgcggatga
      661 agaaatagcc gatcaataaa taggcgactg cgacgaggaa accggataat accggccaga
      721 aattatgact gcggttgctg aatagcccga caataagcgc aatgcccgag cagattaata
      781 atgcacccac tactgtgctt aaaatgtcgc cagagacgaa cggaaaactg atacacagca
      841 acccgacgat aaacagcagc acggcaataa actggattgc tctgcgatgt tttttaagca
      901 tctccagatc aaacttcaa
//
LOCUS       DAZUCC010000530          915 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZUCC010000530 DAZUCC010000000
VERSION     DAZUCC010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.533"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..915
                     /gene="ybdO"
                     /locus_tag="ACHY09_005373"
     CDS             13..915
                     /gene="ybdO"
                     /locus_tag="ACHY09_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3888163.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FINRKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
BASE COUNT          290 a          192 c          146 g          287 t
ORIGIN      
        1 acggagtatg ttatggctaa tctttatgat ctaaaaaaat ttgacctcaa tttactggtc
       61 atatttgaat gtatttatca acatctcagc attagtaaag cggcagaatc attatatata
      121 acgccctctg ctgttagtca gtcgttacaa cgtttacgtg cgcaatttaa tgacccgtta
      181 ttcattcgct cagggaaagg tatcgcgccg actacaacag ggttaaacct gcatcaccat
      241 cttgagaaaa atctcagagg ccttgaacaa acgatcaata tcgttaataa gtctgaacta
      301 aagaaaaatt ttattatcta cggtcctcag cttatttctt gtactaataa caacatgcta
      361 atccgctgtc ttagacaaga tacttcaatc gatatagaat gccatgacat cctgctttca
      421 gctgaaaatg ccgaagaact gttggttcaa cgcaaaactg acctggcaat tacactgcaa
      481 ccagtaatta gtcgctctgt catctgcatg ccattacaca ctattcgcaa ttcccttata
      541 tgtagcaaca aacatcctcg tattactgat accagtaact atgaacaaat tatcgccgaa
      601 gaattcaccc tgttgatatc taaatcagca gggattgacg aaattcaaat ggacatagat
      661 gaacgtttta taaaccgtaa agtttcattt cgcagttctt ctttgttgac tataattaat
      721 agcatttctg ttactgactt attaggcatt gtgcccaccg aactgtacga tttacatcgt
      781 gattttttga agttaaaaga aataaaacta gaacaacctc ttcctgcggt taaactttat
      841 atttcctata acaaggcttc gttgaacaac cttgtcttct cccgattcat agatcgttta
      901 aatgatagct tctga
//
LOCUS       DAZUCC010000531          905 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZUCC010000531 DAZUCC010000000
VERSION     DAZUCC010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.534"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..350
                     /locus_tag="ACHY09_005374"
     CDS             <1..350
                     /locus_tag="ACHY09_005374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888164.1"
                     /translation="KINIKNSAKFNIRSTSRLTYDIISQLHLPQNGYFMERSNFLVAI
                     IMLILRSLAIIPAWFVSLGHEIVTFPFILEIWGNAGDAFSSTSVGNYGFGHYDLNLPQ
                     SQLFAGRYGKQTK"
BASE COUNT          282 a          187 c          166 g          270 t
ORIGIN      
        1 gtaaaatcaa cataaaaaat tcagcaaaat ttaatattcg ttcaactagc cgtctcacat
       61 atgacattat tagtcaactg catttgccac aaaatggcta tttcatggaa cgcagtaatt
      121 tcttagtggc tattatcatg ctaattctta gatccctagc gattattcct gcctggttcg
      181 tcagcttagg ccatgagatt gttacattcc cttttatcct ggagatatgg gggaacgcag
      241 gcgacgcttt ttcatctaca tccgttggta actatggctt tggtcactac gacttaaacc
      301 ttcctcaatc acagctattc gccgggcgat acggcaaaca aactaagtga aacatcatcc
      361 tgaaaacacc aacatcaaca agcctctcca gatcgacatc aggagtgacc agttacaagc
      421 cacaaaccaa gaaacatatt tcatgatgag aattatgctc agcctgatgg cgggtaaaca
      481 cttattttca ccaagctcac tgagttagct tgcaaagctt tctttgttta gattctaaat
      541 aaatttttta taaaacagac agttaaaata aaatctttaa tttttgaaga ccaccgcaag
      601 tgttcgtctg gcttcacatg gcatctgcct cttagaaaaa gatcgacata ttttgtgaca
      661 cgaattgcaa atctcgtttt gttgtatgga ttgcgtgatt tttgatctgg tataacaggt
      721 ataaaggtgc accgcgatag tcaatgagac agggcatctc gcaatctatg gcaaacatca
      781 cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag tctgaaagga atatgaaaat
      841 gagatcaaca gacactctat tttatgactc tgggtaaaat ggattgagta agtgatatag
      901 cttac
//
LOCUS       DAZUCC010000532          903 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZUCC010000532 DAZUCC010000000
VERSION     DAZUCC010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.535"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..297)
                     /locus_tag="ACHY09_005375"
     CDS             complement(<1..297)
                     /locus_tag="ACHY09_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFS3888165.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQ"
     gene            complement(304..645)
                     /locus_tag="ACHY09_005376"
     CDS             complement(304..645)
                     /locus_tag="ACHY09_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888166.1"
                     /translation="MSRIYGRMKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            complement(650..>903)
                     /locus_tag="ACHY09_005377"
     CDS             complement(650..>903)
                     /locus_tag="ACHY09_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFS3888167.1"
                     /translation="TGMAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLAR
                     NSVLTIIERDNERVGNNPMIQQADNADSRIHSLDAPDKE"
BASE COUNT          228 a          290 c          189 g          196 t
ORIGIN      
        1 ctgtgccctg gatttatcga atgacagcgt atccagcgtc acctgtacca gaggaacaca
       61 gcaggatatc atcacaaaaa aagcaccata gagaacatgc tcaatacgcg gcagggaaag
      121 catccctttg tttccctcat ccgcaccttc ttccctgact ttcagccaga tcccgatgat
      181 cctgaatgcc agtggcaggg caaacagtcc tgtgctgatc aacacattcc acagaccgtt
      241 attcaccacc cagcccagca gagtcagaaa atattccaga tagttatttg tggtcatgat
      301 tgctcaacgc cccggctcag caaaaatggc gtattcacac agcaggataa acagaacact
      361 gactgtggcc atacgcagaa aagaagcccg gtattctgcc ctgaaccccg gcgcatgcca
      421 gatcctccac cccagccatc cgatcgcact gtaaagacac agacgccatc ccagccagcc
      481 ataacgtgtc tcatacatcc actcccgcag ggccgcaaat ccttctgcgt gacgtatacc
      541 ggtatcggcc agccagaggg aaaacacggt aatccctgtc agcaacagcg tatacagggc
      601 gaataccttc atcctctttt tcatccggcc ataaattctg ctcatcctct cactccttat
      661 ccggggcatc gagagaatgg atacggctgt cagcattgtc agcctgctgg atcatcgggt
      721 tattccccac acgctcgtta tcccgttcaa tgatggtcag aacactgtta cgcgccagat
      781 cccgcttcag ctccatctcg tttttcagcg ccataatttc ccgatccagc gcctcaatac
      841 ggcgttcccc ctcttccagt gcagcaggct gagctgcagc atacggctcg gccatccccg
      901 tga
//
LOCUS       DAZUCC010000533          892 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZUCC010000533 DAZUCC010000000
VERSION     DAZUCC010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.536"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..843
                     /locus_tag="ACHY09_005378"
     CDS             43..843
                     /locus_tag="ACHY09_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888168.1"
                     /translation="MNSKKVCCICVLFSLLAGCASQSSIDEKKKKAQVTQSNINKNIP
                     PQLTDKDLFGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YHDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKMSNKELSNDHVTEVQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          281 a          167 c          208 g          236 t
ORIGIN      
        1 taaatattct aatcattatg acaggcgagg gagtgtccga ttatgaattc aaaaaaggtt
       61 tgttgcatat gtgtattatt ctcgctgctt gcaggatgtg cctctcaatc ttctattgat
      121 gaaaaaaaga aaaaagctca agttacacaa agtaatatta ataaaaacat tcccccgcaa
      181 ctgacagaca aagatttatt cggtaatgaa accactctgg ctgtatccga ggaggatatt
      241 caggctgcgc ttgaaggaga tgagtttcgt gtgcctctca attctcccgt aatacttgtc
      301 cagtctggca gtcgcgcacc ggaaaccatt atgcaggagg agatgagaaa atattatact
      361 gtttccacat tctctggtat cccggacagg caaaagcctc tgacttgtaa caaaaataaa
      421 gataaaaatg agaacgagga tgttgccagt gctgaaaata tgaactggat gcaggcattg
      481 cgttttgtgg ctgcgaaagg gcatcagaaa gcgattattg tttaccatga tacgttgcag
      541 acaggaaaat atgactctgc gctgaaatca acagtatggt ccgactataa aaatgaaaaa
      601 ctcactgacg ctatctccct gcgctacctg gtacgtttca cgctggtgga tgtggcaaca
      661 ggtgagtggg ctacctggtc gccggtgaat tatgaatcga gagtaatttt tccgccggct
      721 ggaataacaa aaatgagcaa taaagaacta tcaaatgacc atgttacgga agtgcaactg
      781 tttaatttga aacaaaaaac atatgtatcg atggtgaaag acttggtgag tcgattccag
      841 taatcgttat tgttatgctg ccgcctgtag tttagggcgg gcagcaaaca gg
//
LOCUS       DAZUCC010000534          888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZUCC010000534 DAZUCC010000000
VERSION     DAZUCC010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.537"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..460
                     /locus_tag="ACHY09_005379"
     CDS             <1..460
                     /locus_tag="ACHY09_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFS3888169.1"
                     /translation="VPESSLISLMDLNEASLNAASTRAGESKIKHKINHDVFEPYPAA
                     LHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGIVHNSFGQ
                     KLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
     gene            826..>888
                     /locus_tag="ACHY09_005380"
     CDS             826..>888
                     /locus_tag="ACHY09_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888170.1"
                     /translation="MNSILIITSLLIIFSIFSHAL"
BASE COUNT          271 a          199 c          152 g          266 t
ORIGIN      
        1 cgtacctgag agtagtctga tatctttaat ggatttgaac gaagctagcc tgaacgcggc
       61 atctacaagg gctggggaat caaaaattaa acataaaatt aaccatgatg tttttgaacc
      121 ttatcccgcg gcgttacatg gtcaatttga ttccatttcc atgttttacc ttcttcactg
      181 cctgcctgga aatatatcta caaaaagctg tgtaatacgc aatgcggcgc aggccttaac
      241 tgacgatgga actctatacg gagccacaat tcttggcgat ggaattgtgc acaatagctt
      301 cggtcaaaaa ctgatgcgca tttacaatca gaaaggcatc ttttcaaaca caaaagattc
      361 cgaagaaggc ttaacacata tactctcaga gcatttcgag aatgttaaaa ccaaggttca
      421 aggtactgta gtaatgtttt ccgcttcagg gaaaaaatag catccaaccg cagcacgttc
      481 ttgcataagg cgtgtctgcg gtttttcaac tattcagata catcactccc atcacattca
      541 ttcctccgca tcataggcat ataggctata tcaccttgat atttttcttc ttcagataaa
      601 aactgttatc tatgtatact tttaaaccca atccgtgtag agtctctgca taagatagtt
      661 tgcagttgac acttcagctt gcgccataaa ccgcctgatt tttgccgcca cctgttagca
      721 ttcctgtata cctgaaacga caatgtttat ctacgaactt taagaacacc caggataaaa
      781 attgtcaact atatcatata taacacatta ctaattcgag gctatatgaa cagcatactg
      841 ataatcactt cgctccttat catattcagc atttttagtc atgctcta
//
LOCUS       DAZUCC010000535          888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZUCC010000535 DAZUCC010000000
VERSION     DAZUCC010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.538"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(544..>888)
                     /locus_tag="ACHY09_005381"
     CDS             complement(544..>888)
                     /locus_tag="ACHY09_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS3888171.1"
                     /translation="INSVALKVRYIICSSPSRAWRVKDISNSLYMSESLLKRKLQEEK
                     VSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISVFREYFGITPHQMMIN
                     QSGFIESRIAIK"
BASE COUNT          268 a          178 c          176 g          266 t
ORIGIN      
        1 gcaagttatg acagaattac tgaaaagcgt tatctgtagt aattgtagtt tctgatatat
       61 gctcatccat ccggaaatac gacatggtcc tgactggcgt tcacttcaaa gtgtaattta
      121 ttattcacac actgagactg aaaaacagat ggcgtggatg agtacacagt ctgaagagta
      181 taaaaggaac agctccggat tgttacccgg gattactata gttcaccaca caaccgtaaa
      241 cctggttgtg tggtgaaaca gtaacatatt aagaatattg tgttaccacc tgttttctca
      301 ggaaacggat tgcaggagag gtatactcat tcatcttccc ggagttttgt taatcactgc
      361 ataatcaaac ggaccaatac attctttgct cgcatccgta aaaatgtcgc cacaatatca
      421 tcagtcagtc aactcattac catctgtttt ccggggccgt agttactaca gtgctccgga
      481 gtgataaaca gaaactgata atgaagcata agaatacttt tattatgcgc tgactgacat
      541 actttactta attgcgattc tgctctcaat aaatccggac tgattaatca tcatctgatg
      601 aggtgttatg ccaaaatatt ctctgaaaac gctgataaaa tacggtgtgc tggcataacc
      661 gcaccgtatt gcgatttgat tcactgacag tttactggaa ataagttgtc gggcgttatg
      721 catgcgtata tccagcagga tttgggagaa agaaactttt tcctcctgaa gttttctttt
      781 cagtaaactc tcgctcatgt acagagaatt actgatatct ttaactctcc atgcccgtga
      841 tggactggaa cagataatat acctgacttt aagcgcaaca gagtttat
//
LOCUS       DAZUCC010000536          885 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZUCC010000536 DAZUCC010000000
VERSION     DAZUCC010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.539"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            241..624
                     /locus_tag="ACHY09_005382"
     CDS             241..624
                     /locus_tag="ACHY09_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888172.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            693..>885
                     /locus_tag="ACHY09_005383"
     CDS             693..>885
                     /locus_tag="ACHY09_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS3888173.1"
                     /translation="MFFRGHALMKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVP
                     PWKRPWRSARNGVGLPEMMP"
BASE COUNT          190 a          204 c          254 g          237 t
ORIGIN      
        1 ttgacagctt actgctgcag tttttgtccg gtcaggggcg acagttatcc ccggatgact
       61 gggcagcact ggaagaggcc gaatatcagc tttactggct gcgggctctt gccggtgggc
      121 ggtaacagaa cgccgtagtt tctttatccg gaataacgtc atcacgtatt ccgtcattat
      181 atcacccggc agggacatat ccctgtgggg agagtcccgg ctttattcag gagaattgtt
      241 atgggactcg atatttactt tctggctcgt gataaccatc acagtggtgc cgttgtggga
      301 acacaggaac atcaggaggt gggggctttc cgtaaggtga aaccgcttct gacatggttt
      361 cgtgaacata ccggtgcttc agatgagcag catgctttcc ctgtaacccg cagttcactg
      421 gctgcattat tgcgcgacct cagtatgctg acaccgggga actgccacag gctgtttcct
      481 tatgtcacag gctcgtgttt acggggggaa gattatgccg gtgattactg gtatgacgtg
      541 gatgacgtaa aagcgtgggt cagcaatacg ctgaagacgt ttgattttga gcgtcgttca
      601 ctggtgatgc tggcgttgtg gtaattctgg ccagtctgat tttcgttatt aatttaactg
      661 tacggtgcac tctcaccgta cagggagcgt gcatcttttt cagggggcat gctcttatga
      721 aaacgaaaaa acatacttca cgtaccgcga ctaaaactcc ggaacgtgac ctgtatcagc
      781 ttgtgaccga cagggttgtt acggccattg aaaatggtgt gccaccctgg aaacgtccct
      841 ggcgctctgc gcgtaatggc gtggggcttc cggagatgat gccgg
//
LOCUS       DAZUCC010000537          882 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZUCC010000537 DAZUCC010000000
VERSION     DAZUCC010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.540"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>882
                     /locus_tag="ACHY09_005384"
     CDS             <1..>882
                     /locus_tag="ACHY09_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFS3888174.1"
                     /translation="ASQSTGGSASAVPWICGDTTYFWKQRHAEGYASVYGGYKGKESQ
                     NIFFVPLMTDENGANVQTNDPAEDPDLEAVGYYGSKWRNDQKTWTSADRASHFSSWAR
                     RGIISDRLATAILVHAGRTAEFITGKQPDTVKPTGPSGEGPEREPEAPVSNRTLMSLL
                     ASGEDLASQGWRYYHKPASGDSVDKNIAEAVVSDEGATGGKALQLNKPESHIWFLEHD
                     AAGQGAELLKKGGRVSVRFKVPGALVPNQFALGIYWQLSSLPEGVTLSEEGNDMLMSF
                     FLQTDATNLNAMYHKKQ"
BASE COUNT          217 a          211 c          287 g          167 t
ORIGIN      
        1 ggcatcacag agcacaggag gcagcgcatc agcggtaccg tggatttgcg gggacacaac
       61 gtatttctgg aagcagcgtc atgcggaagg gtatgcatca gtatacggag ggtataaggg
      121 taaggagtcg cagaacattt tctttgtacc gttaatgacg gatgagaatg gtgcgaatgt
      181 gcagaccaat gacccggcag aagacccgga cctggaagcc gtcggttatt acggttcaaa
      241 gtggcgtaat gaccagaaga cctggacatc agcagacagg gccagccatt tcagttcatg
      301 ggctcgccgt gggattattt ccgaccgtct ggcaacggcg attctggtgc atgccgggag
      361 aaccgctgaa ttcattaccg gaaaacagcc tgatacggtg aagcccaccg gaccttccgg
      421 tgaaggcccg gagagagagc cggaagcccc ggtcagtaac cgaaccctga tgagtctgct
      481 ggcgtccggt gaagacctgg catcacaggg ctggcgctat tatcacaaac cggcgagcgg
      541 agacagtgtt gataaaaaca tcgctgaggc tgtggtcagt gatgagggtg ccaccggtgg
      601 taaggccctg caactgaaca aaccggaaag ccacatctgg tttctggagc atgatgccgc
      661 cgggcagggg gcagagttgc tgaagaaagg cggacgtgtg agcgtgcggt ttaaggtgcc
      721 gggcgcactg gtgccgaatc agtttgccct gggtatttac tggcagttgt cgtccctgcc
      781 ggagggagtg acgctgtcag aggaaggcaa cgacatgctg atgtccttct tcctgcagac
      841 ggatgcgacg aacctgaacg cgatgtacca caagaagcag aa
//
LOCUS       DAZUCC010000538          877 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZUCC010000538 DAZUCC010000000
VERSION     DAZUCC010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.541"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..564)
                     /locus_tag="ACHY09_005385"
     CDS             complement(<1..564)
                     /locus_tag="ACHY09_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3888175.1"
                     /translation="MIKIENLTKSYRTSTGRHYVFKDLNIELPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDRGKIITNKTISWPIGLAGGFQGSLTGRENVKFVARLYAKPEELK
                     DKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAHYFKERHKESSFLMVSHSLNSLKE"
     gene            complement(561..>877)
                     /locus_tag="ACHY09_005386"
     CDS             complement(561..>877)
                     /locus_tag="ACHY09_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3888176.1"
                     /translation="CSVGLIFMVVGKTFPEMQKILPIFLKPLYFISCIMFPLHSIPQQ
                     YWTYLLWNPLVHVVELSREAVMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAML
                     TS"
BASE COUNT          262 a          199 c          163 g          253 t
ORIGIN      
        1 ctctttcaaa gaatttaaac tatgagaaac cattaaaaaa ctagactctt tatgcctttc
       61 tttaaagtaa tgtgcacatt tttctttaaa cctggcatca ccgactgccg ttacttcatc
      121 gacaatataa taatcaaact taaatgccat gcttaaacca aaacccaggc gagatcgcat
      181 accagatgag taagttttga tcggcatatc aaagtactta ccgagttcgg caaattcttc
      241 gacaaactca attttatctt tcagttcttc tggcttcgca tataaccgtg caacaaattt
      301 tacgttttct cgcccggtta aactgccctg gaatccaccg gcgagcccaa tgggccatga
      361 tatcgtttta ttggtgatga tctttcctct atcggggcgg tcaataccgc caatcattct
      421 cagtaaagtt gatttacctg caccattacg accaataaag gcaacacttt ttcctgaagg
      481 gagttcgata tttaaatcct taaacacata gtgtcgacct gttgacgtgc gatatgactt
      541 cgtcagattc tcaatcttaa tcatgatgtc agcattgcct cttcacgcgt tcggtatagc
      601 gccagaccga tgaacagggt gaccagagtg aacattgcca gatagttcaa actcacgcct
      661 tcgctgatat agcccggcat aactgcctca cgacttaact ctacgacatg gactaatggg
      721 ttccagagta gatatgtcca gtattgctgt gggatcgaat gtagagggaa catgatacag
      781 gaaatgaaat acagcggttt aagaaatatc ggtagaatct tttgcatttc agggaaagtt
      841 ttaccaacga ccataaatat taatccgaca ctacatg
//
LOCUS       DAZUCC010000539          871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZUCC010000539 DAZUCC010000000
VERSION     DAZUCC010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.542"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>871
                     /locus_tag="ACHY09_005387"
     rRNA            <1..>871
                     /locus_tag="ACHY09_005387"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          221 a          197 c          285 g          168 t
ORIGIN      
        1 gagtaatgtc tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata
       61 ccgcataacg tcgcaagacc aaagaggggg accttcgggc ctcttgccat cggatgtgcc
      121 cagatgggat tagctagtag gtggggtaac ggctcaccta ggcgacgatc cctagctggt
      181 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
      241 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      301 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      361 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      421 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      481 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      541 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      601 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      661 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      721 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      781 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      841 tttaattcga tgcaacgcga agaaccttac c
//
LOCUS       DAZUCC010000540          860 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZUCC010000540 DAZUCC010000000
VERSION     DAZUCC010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.543"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..589
                     /locus_tag="ACHY09_005388"
     CDS             29..589
                     /locus_tag="ACHY09_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3888177.1"
                     /translation="MIEENGNFSFDAPGKESFKARLLSLIGTRSVRAAAKDWGVPVST
                     LNNYIHKGTEPSFKMACLISNKEHVSLNWLAFGSDENTPSTPCCSTVDAGNLDNANQT
                     ITAILSSLSTDEQLELARLLGRKGADFLTILLDDNIQELHALSGFRRELALRLKDMSD
                     SEAREIYEECEAKVNHLNVTQKKASA"
BASE COUNT          271 a          152 c          181 g          256 t
ORIGIN      
        1 tttcaatgtg ttatgaggaa aagaaaaaat gattgaagag aatggaaatt tttcttttga
       61 tgcaccagga aaagaaagtt ttaaagcccg cttactaagc ctgatcggga cgcggagtgt
      121 aagggcagca gcaaaggatt ggggggttcc tgtatcaacc ttaaataact acatacacaa
      181 aggcacagaa ccctctttta aaatggcttg ccttatatca aataaagagc atgtgagttt
      241 aaattggtta gcttttggtt ctgatgaaaa taccccaagc actccatgtt gttcaactgt
      301 cgatgcaggt aatttagata atgcaaatca aacaattacg gcaattctgt cctctctcag
      361 caccgatgag caactagaat tagcccgatt gcttggcaga aagggggctg attttctaac
      421 gatcttattg gatgataata ttcaggaatt acatgcactt agcggttttc gccgagagct
      481 tgctttaagg ttaaaggata tgtcagattc tgaagcaaga gagatttatg aagaatgtga
      541 agctaaggtt aatcatttaa atgtaaccca gaaaaaggct agtgcgtaag ggagtggtgc
      601 aaattttgtg ctgtggtaca ctcctttaaa gacaatctgg agaaaagcgt tttaagcctt
      661 tttaaaacca ttttaagcgt caatccgtag tatcaaaagt gttgccgatt ttagaataaa
      721 cgactaatta ttgcacaatc ggtatcaaat agactttccg cgctcgcatt ggtatcacct
      781 gttttatttc agtatcttac aggtgatttc acctcctttc actaaaaccc tcgttggtat
      841 caaatgattc acctggttac
//
LOCUS       DAZUCC010000541          857 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZUCC010000541 DAZUCC010000000
VERSION     DAZUCC010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.544"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..93
                     /locus_tag="ACHY09_005389"
     CDS             <1..93
                     /locus_tag="ACHY09_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS3888178.1"
                     /translation="RDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            367..>857
                     /locus_tag="ACHY09_005390"
     CDS             367..>857
                     /locus_tag="ACHY09_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFS3888179.1"
                     /translation="MNMITVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPGIRDKVIQYQEEC
                     DDVLYEYWTKGFVVNPRQMSVMEELNQACADMKRDKNIASLFGTGLNEWKLVKAAHVS
                     KIRT"
BASE COUNT          246 a          189 c          221 g          201 t
ORIGIN      
        1 agagactgcc gtattcgctc aaataattgc tacggtcagg tatcccgtcg cgaccaggag
       61 agtgcgctgg catgctgggg tatcgacagg tagcagaata ttttgctgaa aaatgacgtt
      121 gaccaacgcg gacggataac acgaaatcct gcgaactggc aaaacctaag tgaataaagt
      181 caggaacatt gtttcacgca gaggcaccgt aatggtgcct ttggcatttc tgcgcttcgc
      241 acaagcgtaa ataaaccaaa gaacctttca ggatgagccc tggtggataa ccggcagtgg
      301 tctggttaac cctctttggg ctggttattc ctgtgcgcag ggttcatcac taaaaggaaa
      361 taaccgatga atatgataac cgtgccgttt cacggcgatt ctctttatgt ggttaaccat
      421 aacggcgaac catacgttcc catgaaacct gtcgttgcgg ggatggggct ggcctggcaa
      481 tcacagttgg ctaagttaag acagcgtttt gcgtcaacta taacggaaat cgttatggtt
      541 gctgaggacg ggaaacgacg caatatggtg tccctgccac ttcgaaaact tgcaggctgg
      601 ttacaaacca tcaatcccaa caaagtaaaa cccggaatcc gcgacaaggt tatccagtat
      661 caggaagagt gcgacgatgt tctctatgaa tactggacga agggttttgt cgttaatcct
      721 cgtcaaatga gcgtgatgga agaactcaac caggcatgcg ctgacatgaa acgggataaa
      781 aacattgcca gtctgtttgg tacagggtta aacgaatgga aactggttaa agccgcgcat
      841 gtatcaaaaa ttcgcac
//
LOCUS       DAZUCC010000542          856 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZUCC010000542 DAZUCC010000000
VERSION     DAZUCC010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.545"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..166
                     /gene="cdiI"
                     /locus_tag="ACHY09_005391"
     CDS             <1..166
                     /gene="cdiI"
                     /locus_tag="ACHY09_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease toxin immunity protein CdiI"
                     /protein_id="HFS3888180.1"
                     /translation="GVEFAVGYPPDEDDIVIVSEETCFEYVRLACEKYLQLHPEDTEK
                     VNKLLSKIPS"
BASE COUNT          261 a          202 c          161 g          232 t
ORIGIN      
        1 aggtgttgaa tttgctgtcg gctatccccc agatgaagat gatatagtga tcgtaagcga
       61 agaaacctgt tttgaatatg ttcgtctggc ctgtgaaaaa tatcttcagt tacaccctga
      121 agatactgaa aaagtaaaca aattactgtc taaaatccca tcctgagtaa agattccggc
      181 cactgcgccg ggatctttct ttaaccgtca ggctcatcca caatgtccat ccccacgctt
      241 aatcaccggc acatctctaa tgtcgttaag cgcaccgggg aaggcgttaa accacgtgag
      301 catctgcggc ctgttgacat acgttgtgtc gttacgggga agtcatcttc acagcgattt
      361 tcagtcagtt agcattaccg acattcattc atcaaattac cggagataat tgatgtcaaa
      421 accgtaagcg taaactgacc gccgtatgta gccatcagac gagaattggt aacttagacg
      481 cccatctgat atagacggac atctaagtat ggaattacag gactggcgaa aagaacctcg
      541 taaaaactat tcgaatgaat tcaaacttcg tatggtggaa ctggcatcac aacctggagg
      601 tttatcccaa gaatattgcc tttgatatgc tggcacatac aatccttgac cattctcaca
      661 gatatactcc cacacacttt tgggttgcgc cccgaaaaca tctttgattt ccatataacc
      721 ccttgattta ataccattta tcaaaagttt aacatttcgg aattttacca actatggaag
      781 acttagcaat aaccatcaaa caatactaac taatcatccc ttagcaaggg ctaactctac
      841 gaaacgctat gctgct
//
LOCUS       DAZUCC010000543          856 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZUCC010000543 DAZUCC010000000
VERSION     DAZUCC010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.546"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..577
                     /gene="slp"
                     /locus_tag="ACHY09_005392"
     CDS             11..577
                     /gene="slp"
                     /locus_tag="ACHY09_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFS3888181.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            730..>856
                     /locus_tag="ACHY09_005393"
     CDS             730..>856
                     /locus_tag="ACHY09_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3888182.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAF"
BASE COUNT          248 a          188 c          188 g          232 t
ORIGIN      
        1 ggatagtaac atgaacatga caaaaggtgc actcatcctc agcctttcat ttttgcttgc
       61 cgcatgtagt tcaattccgc aaaatatcaa aggcaataac caacctgata ttcaaaaaag
      121 ttttgttgct gttcataacc agccggggtt atatgttggt caacaagcgc gctttggcgg
      181 gaaggttatc aacgttatca atggcaaaac ggatacgttg ttagaaatcg ctgtattacc
      241 gttggatagc tatgcgaagc ctgatattga agccaactat cagggccgac tgctcgccag
      301 acaaagcggc ttccttgatc cagtgaacta tcgtaatcac tttgttacca tcctcggtac
      361 cattcagggt gaacaacctg gctttatcaa taaagtcccg tataacttcc tggaagtgaa
      421 tatgcagggc atccaggtgt ggcatttgag agaagtggtt aataccacct ataacctgtg
      481 ggattacggc tatggtgcat tctggccgga accgggctgg ggcgcgcctt actacaccaa
      541 tgcggtgagt caagtaacac ctgagctggt caaataacac caccgagaga tgcagatgct
      601 ttctcagcat ctgcatcatg cattacatca aattaataca cagccagcta actattatta
      661 taagccctgt cctgttaatt acctttggca aactgattat aaagttaatg tccgcaccag
      721 gagtcggtta tgtttcttat aattaccagg gatacgatgt tcttcactgc gatgaaaaac
      781 attctgagta aaggtaatgt cgttcatata cagaacgaag aagagatcga cgtaatgttg
      841 catcagcatg ccttcg
//
LOCUS       DAZUCC010000544          856 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZUCC010000544 DAZUCC010000000
VERSION     DAZUCC010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.547"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            189..791
                     /gene="fimB"
                     /locus_tag="ACHY09_005394"
     CDS             189..791
                     /gene="fimB"
                     /locus_tag="ACHY09_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFS3888183.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          252 a          162 c          188 g          254 t
ORIGIN      
        1 tggcgtttgt atggcaaagt tattataagt aactatcgct actccattta agttcgctca
       61 gaagaactgg tccacttacg ttagttatta agcaaacgtt cgcttttata aacataatca
      121 ggataaaaat gttggattat tgctaaccca gcacagctag tgcgcgtctg taattataag
      181 ggaaaacgat gaagaataag gctgataaca aaaaaaggaa cttcctgacc catagtgaaa
      241 tcgaatcact ccttaaagca gcaaataccg ggcctcatgc agcacgtaat tattgtctga
      301 ctttgctttg ttttattcat ggtttccggg cgagtgaaat ttgccgattg aggatttcgg
      361 atattgatct taaggcaaag tgtatatata tccatcgatt aaaaaaaggc ttttcaacaa
      421 cgcacccgct attgaacaaa gaaattcagg ctttaaaaaa ctggttgagt atccgtactt
      481 cttatccgca tgctgagagc gagtgggtat tcttatcacg taaggggaat ccactttctc
      541 ggcaacagtt ttaccatatt atctcgactt ccggtggtaa tgccgggttg tcactggaga
      601 ttcatccgca catgttacgc cattcgtgtg gttttgcttt ggcgaatatg ggaatagata
      661 cgcgacttat ccaggattat cttgggcatc gcaatattcg tcatactgtc tggtataccg
      721 ccagcaatgc agggcgtttt tacggcatct gggatagagc cagagggaga cagcgtcacg
      781 ctgttttata gagatagttg agataccagg gatggtgtta aaaactacat tattcttacg
      841 atatatcaaa aatgat
//
LOCUS       DAZUCC010000545          854 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZUCC010000545 DAZUCC010000000
VERSION     DAZUCC010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.548"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>854
                     /locus_tag="ACHY09_005395"
     CDS             <1..>854
                     /locus_tag="ACHY09_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFS3888184.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGPDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNLHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDMQGHEMRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TQISHLSEG"
BASE COUNT          216 a          249 c          244 g          145 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacaccggat ggcagggcat cggcatttta ctataaccac
       61 cacagccagt taacgtcagc caccgggcct gacgggctgg aattgcgccg ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatctac acagtgactt accctgcgca acggaagatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacat gcagggccat
      421 gaaatgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggcttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac acaaatcagc
      841 catctcagcg aagg
//
LOCUS       DAZUCC010000546          854 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZUCC010000546 DAZUCC010000000
VERSION     DAZUCC010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.549"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>854
                     /locus_tag="ACHY09_005396"
     CDS             <1..>854
                     /locus_tag="ACHY09_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFS3888185.1"
                     /translation="GLITRITTPDGRASAFYYNHHSQLTSATGTDGLELRREYDESGR
                     LIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLTVTDCSGYVTRY
                     DHDRFGQMTAAHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDG
                     SRNGTQYDTWGKAIRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQET
                     GFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRITHRTVKGETAEQWRYDERGWL
                     TGISHLSEG"
BASE COUNT          212 a          250 c          249 g          143 t
ORIGIN      
        1 ggactcatca cgcgcatcac cacgccggat ggcagggcat cggcgtttta ctataaccac
       61 cacagccagt taacgtcagc caccgggact gacgggctgg agttgcgcag ggaatatgat
      121 gaatccggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg ctaccgttat
      181 gataatccac acagtgactt accctgcgca acggacgatg ccaccggcag ccggaaaacc
      241 atgacatgga gccgttacgg tcagttgctg accgtcactg actgttccgg ttatgtaacc
      301 cgttatgacc atgaccgttt tggtcagatg acggcggcgc accgcgagga agggctgagt
      361 cagtatcgcg catacgacag ccgtggacag ttaattgcgg tgaaagacac gcagggccat
      421 gaaacgcggt atgaatacaa cgccgccggt gacctgaccg ccgtcattgc cccggacggc
      481 agcagaaacg ggacacagta cgatacgtgg ggaaaggcca tccgtaccac gcagggtggt
      541 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      601 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggtttt
      661 gacggccgca cacagcgtta tcatcacgac ctgaccggta aacttatccg cagcgaggat
      721 gaggggctgg tcacccactg gcactatgac gaagcagacc gcatcacgca ccgcaccgtg
      781 aagggtgaaa ccgcagaaca gtggcggtac gacgaacgcg gctggctgac aggcatcagc
      841 catctcagcg aagg
//
LOCUS       DAZUCC010000547          848 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZUCC010000547 DAZUCC010000000
VERSION     DAZUCC010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.550"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHY09_005397"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHY09_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3888186.1"
                     /translation="MKIYRPLWSEGVLLSPQQFQQQTEWESFRSAGISALASPFPWGV
                     EKVELNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADHDAVTVV
                     IALPHMQPGVINVEQ"
     gene            complement(369..>848)
                     /locus_tag="ACHY09_005398"
     CDS             complement(369..>848)
                     /locus_tag="ACHY09_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFS3888187.1"
                     /translation="PETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYDTPDA
                     TANSRINARLPYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNNWIRGLVTEMTDP
                     GDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
BASE COUNT          209 a          238 c          215 g          186 t
ORIGIN      
        1 tcctgctcaa cattaatcac gccaggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgtggt cagccagctg accggcatct aactctcgcg gtgccggagg caaatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggatg acagaagact gtcattaagc tcaacctttt ccactcccca gggaaacggg
      241 gatgccagcg cagaaatacc ggcactgcga aaagactccc attcagtctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggtaaatctt catttcccga
      361 cgcctgcctt acgcttttgc cttcggcatc tgcgaaacca gggacagact gacatccata
      421 ccttccacct ggaaatgcgg tacagcgtag agtttcacac ggaaaaatcc cgggttgtct
      481 tcgatatctt ccaccaccac ttttccgtca cgcagcggat gcgaggcctg caactcatca
      541 cccggatcgg tcatctccgt caccagaccc cggatccagt tattcagctc cagctcaagc
      601 agacggcgat ctttggtggt accgatattt tcccgctgaa tcatcttcag gtaatgcgca
      661 atgcgggaca gcaggaagat atagggaaga cgggcattaa tacggctgtt cgccgttgca
      721 tccggcgtat cgtacagtgc cggtttctgg gtggagttag cggagaagaa gcaggcataa
      781 tcccggttct tgtagtaaga gagcggaata aagcccagat tagcgaattc aaactcccgc
      841 gtttcagg
//
LOCUS       DAZUCC010000548          845 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZUCC010000548 DAZUCC010000000
VERSION     DAZUCC010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.551"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..224
                     /locus_tag="ACHY09_005399"
     CDS             33..224
                     /locus_tag="ACHY09_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888188.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            238..539
                     /locus_tag="ACHY09_005400"
                     /pseudo
     CDS             238..539
                     /locus_tag="ACHY09_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(539..634)
                     /locus_tag="ACHY09_005401"
                     /pseudo
     CDS             complement(539..634)
                     /locus_tag="ACHY09_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          161 a          260 c          261 g          163 t
ORIGIN      
        1 atgcgtcagg cgctggacgg ggagaacggg taatgaatat cagcacggaa acccgcgaaa
       61 ttctgcgtaa ttacaaagct gtgattaatg cgcggcgtcg tgaaatgggg cagaaaccgc
      121 tcaccaccgc gcagattgtt gatgaaatct gtgattttgt ggcgaatcag caggcggttt
      181 tcctcggtgg tcactatatc cttcagggca gcagaaacag gtgatacagg gcaggcggtg
      241 gaggccgcct gtcagcccgc gcgaaaaccc ctggcaggct ggcgcctgcc ggaagggaag
      301 cgggccacgc tggcgcgtcg cccgttaccc ttcctgttgg tgacaggcgt tgtttcagga
      361 agtacctgtt acagacctgt cgttaaagaa ccttcttaca catgcgcccc ttcggctccc
      421 ggctgcctgg cggcatccgg tcgtcggccc gactacgaca ggcagctgct ggcgcacccg
      481 ccagtctccg ccgaacccgc tggcgcggtt tcgggctgtc gctacggtcc gatgcgtgaa
      541 aacccgggct tgcagcgacc ggaacggtcg cgccgccttt gtcgcgccgt ggcctgcgct
      601 cctggcatcc cggcaaaagg ccgccgggat gtaaggaaac cgcgcctgat ggcgcggcag
      661 tctcctcttc tgccgccacc tctgcggcct gtttcccgct gtggactttc cggggggcag
      721 acgtaaccac ctcccgttcc atcagtccag gggaagatat accggccaca aaatttttag
      781 ccatgaaccc ggcaaaaaat tttgcgtccg cccccggaca gctgtcccgt gaggcggtaa
      841 acggg
//
LOCUS       DAZUCC010000549          844 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZUCC010000549 DAZUCC010000000
VERSION     DAZUCC010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.552"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..268
                     /locus_tag="ACHY09_005402"
     CDS             <1..268
                     /locus_tag="ACHY09_005402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888189.1"
                     /translation="TEKRRTGMEEMQDKMAWMQNVITEKLGPSLWHMHPIAFPGALIK
                     KNTGWAHSKFANLLGSVELENDYSAYNVTKTGKSVPHYNINLTK"
     gene            439..>844
                     /locus_tag="ACHY09_005403"
     CDS             439..>844
                     /locus_tag="ACHY09_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888190.1"
                     /translation="MGCNKKSNKFLLFVSCMLLSFVTYSQDSSNPQFSDYLVPVSNGP
                     FEKNIYFNKEQENYSQHWKNAVQEELKKPVNFAGHFRIYTAFGGHGKECLRDNWVCGW
                     VIDKLSGEVVATLPSDNNGSYNYADVSDNGTPV"
BASE COUNT          277 a          137 c          194 g          236 t
ORIGIN      
        1 cactgaaaaa agacgtaccg gaatggaaga aatgcaggat aagatggcct ggatgcagaa
       61 tgtgatcacg gagaaactgg gcccttcgtt gtggcatatg catccgattg cctttcctgg
      121 agcattaata aagaaaaata ctgggtgggc gcatagcaag tttgcaaacc ttttgggaag
      181 tgtcgagtta gaaaatgatt attcggcata taatgttact aagacaggta aatctgtccc
      241 gcactataat attaatctca caaaatgaca ttatctgagg taattgatgc cccaagagaa
      301 cgaaaaatgg taaaaagaaa tacaggttag tacctagggt ggcagaagtt gagggggagt
      361 cgtactattc tggtgatgga ttgaataaag ctcacctatt accagatacg atgattaaag
      421 ctcttgagga ttctaaaaat gggatgtaat aaaaaatcaa acaaattttt gctttttgtg
      481 tcatgcatgc tcttgtcgtt tgtaacgtac tcacaagaca gttctaatcc tcagttttct
      541 gactacctgg ttcctgtaag caacggacca tttgaaaaga atatttactt taataaagag
      601 caggaaaatt actcgcaaca ctggaagaat gcagtccaag aggaactaaa aaagcctgtt
      661 aattttgcag ggcactttag aatctataca gcctttggcg gtcatggtaa agaatgcctt
      721 cgtgataatt gggtatgtgg ttgggttatt gataaactat caggggaagt tgtagcaaca
      781 cttccatcag ataataatgg aagttataac tatgctgatg tgtctgataa tggtacccct
      841 gttg
//
LOCUS       DAZUCC010000550          843 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZUCC010000550 DAZUCC010000000
VERSION     DAZUCC010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.553"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..229
                     /locus_tag="ACHY09_005404"
     CDS             <1..229
                     /locus_tag="ACHY09_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFS3888191.1"
                     /translation="PLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLA
                     KKSSAWGGGRKTFIREAHGWSLRRTRSRGKK"
     gene            226..>843
                     /locus_tag="ACHY09_005405"
     CDS             226..>843
                     /locus_tag="ACHY09_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFS3888192.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQR"
BASE COUNT          170 a          209 c          276 g          188 t
ORIGIN      
        1 tcctctactt ggctgggtga ttatcccgac agggatgttg ctgactcccc tgctgctggt
       61 ctggttcggt ggcgtctgga tcagccggct gaagcgggga aaacctgaca actggctctg
      121 gcagcatctg gcgaagaaaa gcagtgcatg gggtggtggc cggaaaacat ttatccggga
      181 ggcgcatggc tggtccctgc gccgtacccg tagcaggggg aaaaaatgag tcgttttcgt
      241 catgctctgc gggatcgcga ccagcatatc ctgacactgc gtattttcag cggtgtgctg
      301 gccggaattc tgatgctggt ctgtatcggg tggatgacgg caccggacag actgacaatt
      361 cacaatccgc cggatctgcg ttccggcagt acccgtccct ggtgggaagt cccgccgtca
      421 acggtttatt cctttgcctt ttatatcttt cagcaactga atgcctggcc gaagaacgga
      481 gaggtggact acccggcgaa aattcatgcg ctctccgcct acctgacgcc atcctgtcgg
      541 gagattctgg agagtgatgc ccgccgtcgc agtgatgcgg gggagctgct ggagcgtgtg
      601 cgcgtggtgt atgaaattcc ggaacatggc tacgggggca ggtacaacaa aaatccggtg
      661 acggtggaag ggcgggataa ctggcaggta cagctggatc tggtggcaga tgagtatttt
      721 catgctgagc cggtaaaacg ggcacttgtc cgttatccga taaaggtggt gcgcatggag
      781 ggggatcctg aacgtaatcc gttcgggctg gcgctggact gctacagcag tacaccacag
      841 cgg
//
LOCUS       DAZUCC010000551          840 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZUCC010000551 DAZUCC010000000
VERSION     DAZUCC010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.554"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>840
                     /locus_tag="ACHY09_005406"
     CDS             <1..>840
                     /locus_tag="ACHY09_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS3888193.1"
                     /translation="QQEGSEDGHGVVFPAELVRLLDRLEEEIRTDRVSSESRAWLAQC
                     GLTVEQLEKQVEPEYTPARKVHLYHCDHRGLPLALISEDGNTVWSAEYDEWGNLLNEE
                     NPHHVYQSYRLPGQQHDEESGLYYNRNRYYNPLLGRYITQDPIGLAGGWSLYAYPLNP
                     VNGIDPLGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKRLNLGGTDQFFHCMAFCR
                     VSKLNDAGVSRSAKGLGYEKEIRDYGLNMFGMYGRKVKLSHSEMIEDNKKDLAVNEHG
                     LTCP"
BASE COUNT          237 a          155 c          258 g          190 t
ORIGIN      
        1 cagcaggaag ggagcgagga cggacacggc gtggtgttcc cggctgaact ggtgcggctg
       61 ctggacaggc tggaggaaga aatccggaca gaccgcgtga gcagtgaaag ccgggcgtgg
      121 cttgcgcagt gcgggctgac ggtggagcaa ctggaaaaac aggtggagcc ggaatataca
      181 ccggcgcgaa aagttcatct ttaccactgc gaccaccggg gcctgccgct ggcgcttatc
      241 agcgaagacg gcaatacggt gtggagcgcg gagtatgatg aatggggaaa cctgcttaat
      301 gaggagaacc cgcatcatgt gtatcagtca taccgtctgc cggggcagca gcatgatgag
      361 gagtcggggc tgtactacaa ccggaaccgg tactacaatc cgttgctggg acgatatatt
      421 actcaggacc cgattgggct ggcgggggga tggagtctgt atgcgtatcc gctgaatccg
      481 gtgaatggta ttgatccatt aggattaagt cctgcagatg tagcgttaat gcgaaaaaaa
      541 gaacaattaa accatcaaag agcatgggat atattatctg atacttatga tgatatgaag
      601 agattaaatt taggtgggac tgatcaattt ttccattgta tggcattttg tcgagtgtct
      661 aaattaaatg acgctggtgt tagccgatca gcgaaagggc tgggttatga aaaagagatt
      721 agagattacg ggttaaatat gttcggtatg tacggcagaa aagtaaagtt atcccattct
      781 gagatgattg aagataataa aaaagacttg gctgtaaatg aacatgggtt gacatgtcca
//
LOCUS       DAZUCC010000552          834 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZUCC010000552 DAZUCC010000000
VERSION     DAZUCC010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.555"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            351..>834
                     /locus_tag="ACHY09_005407"
     CDS             351..>834
                     /locus_tag="ACHY09_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888194.1"
                     /translation="MNISVEDLFPESFQRIKEEEEANRPDPNWFAHLPPEKINAYMSK
                     YPFKSFDDIPLDNSGLQYPSLQELEFYFPPAALAANCHQLPERMRQKPVVTMINGLTL
                     LRSLGVQAFNIDPRRWHKIKTYLSQGTIEYPQMSEDGKVIIDGRHRILLIMQIYKVTD
                     V"
BASE COUNT          244 a          166 c          189 g          235 t
ORIGIN      
        1 gctgtgtttt ctctgtcaag actgtggatt tcaatttttg tgaaaacgat caaaaaacag
       61 gcattcacac cacgtgctat tctcgcctga taccacaaaa aacggcacag gcataactgt
      121 tctctggcct cagcatgttt taatgaaact atgatatgag acacctgggg ggatactgtc
      181 actttatggc atggtacaca tcgtgatatg tgtacaggcc ttgttagtta ccgtaccggt
      241 caggacggac gacgggagtt tgaaaccagt gagttgttct gtactggcgt gttttataat
      301 gaaatcctta tcggtggcga tgagcaatgc tctcaaggga ggatattgtg atgaacataa
      361 gtgtggaaga tttgtttccg gagtcatttc aacgaattaa agaagaagaa gaagcgaaca
      421 ggcctgatcc taactggttt gcacatttac cgcctgaaaa aatcaatgcg tatatgtcca
      481 agtatccttt caaatccttt gatgatattc ctctggacaa cagtgggttg caataccctt
      541 cactacagga actggagttt tattttcccc cggcggccct ggctgctaat tgccatcaac
      601 ttcctgagag aatgagacaa aagcctgtgg tcacgatgat taatgggtta accctgttaa
      661 ggtcgcttgg cgttcaggct ttcaatatcg atccacgccg atggcacaaa attaaaacct
      721 atttaagtca aggaacgatt gaatatccac aaatgtccga agacgggaaa gtaattatag
      781 atggcaggca tagaatacta cttattatgc agatttataa agtaactgat gttc
//
LOCUS       DAZUCC010000553          834 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZUCC010000553 DAZUCC010000000
VERSION     DAZUCC010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.556"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..683
                     /gene="ybcL"
                     /locus_tag="ACHY09_005408"
     CDS             132..683
                     /gene="ybcL"
                     /locus_tag="ACHY09_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFS3888195.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATVTYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            693..>834
                     /locus_tag="ACHY09_005409"
     CDS             693..>834
                     /locus_tag="ACHY09_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS3888196.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETH"
BASE COUNT          256 a          142 c          198 g          238 t
ORIGIN      
        1 tgtatattgt ggcaaataat tgggttgggg gtacaattgt gattgctttt gcatgaacat
       61 tgcgccttta tgcataatga gataaaggaa tatcaaataa aataacgata ggtcataaca
      121 aagaggtttt tatgaaaaca cttatcgttt caactgtatt ggcattcata acattttctg
      181 cgcaggctgc agcatttcag gtcactagta atgaaataaa aacaggagag caacttacaa
      241 cgtctcatgt cttttctgga tttgggtgtg aaggtggtaa tacatcgccc tcattaacct
      301 ggtctggtgt tcctgaaggt accaaaagct ttgccgtaac tgtatatgat ccagatgcac
      361 ctacaggcag tggttggtgg cattggactg ttgttaatat tccagcaaca gtaacatatt
      421 tgcccgttga tgcagggaga cgtgatggaa caaaactgcc gactggtgct gttcaaggcc
      481 gaaatgattt tggctatgct gggtttggtg gcgcatgtcc tcctaaagga gataaaccac
      541 atcattacca gtttaaagta tgggctctaa aaactgaaaa gattcctgta gattctaact
      601 ccagcggagc gttagttggt tatatgctta atgctaataa aatcgcaacc gctgagataa
      661 caccagttta tgagataaag tagggtgaga gtatgctggc aagaggtaag actaacttaa
      721 agatcgaaga aatacggatg cataaacatc atgagattca tagggttaag cctcttatgc
      781 cagctttgtg tcgtatccgt cagggaaaga aagttatcaa ttgggagacg cata
//
LOCUS       DAZUCC010000554          832 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZUCC010000554 DAZUCC010000000
VERSION     DAZUCC010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.557"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..607
                     /locus_tag="ACHY09_005410"
     CDS             5..607
                     /locus_tag="ACHY09_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFS3888197.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFPDDVTRGEKMRKWLLTICLMFI
                     NVTCKAGDCFDLAGRDYKIDPDLLRAISWKESRNRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTADPCLNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYSGIKNSKGIQLRASEKPLSKMNSSQKN"
BASE COUNT          243 a          178 c          205 g          206 t
ORIGIN      
        1 gggtgtggtg cttttggtgg tgagaaccac caacctgttg agcctttttg tggagtgggt
       61 taaattattt cctgatgacg tcaccagagg tgagaaaatg agaaaatggc ttctgacgat
      121 ctgcctgatg tttataaatg tgacctgcaa ggctggtgat tgctttgatc tggctggcag
      181 agattataaa attgaccctg acttgctgag agcaatatca tggaaagaat cccgtaaccg
      241 ggttaatgcc atcggtatta atccggttac gggatatggc agtggactta tgcaggtaga
      301 ttcccagcat tttaacgaac tggcccgcta cggaattaaa ccggaacatc tgacagcaga
      361 tccctgtctg aacatttata ccggtgctta ttatctggct atagccttta aaaaatgggg
      421 agtctcctgg gaggctgttg gtgcatacaa tgccggattc aggaagaccg aacgccagaa
      481 ccagagacgt cttgcctacg catcagaggt ttaccggatt tacagcggga taaaaaacag
      541 caaggggatc cagcttcgtg catctgaaaa accgctttca aaaatgaaca gctcgcagaa
      601 gaattaattt gatatacatt ttaaaaaacc ggttatgccg caaacgcggc atggaaggcc
      661 acacacaaaa atatcatatg agtgataatg ctgactggcc gaagccccgc aaagcgggaa
      721 cccatcttat atatatccgg taactgcgca gcctcctgcc agaccatttt tcgcgggcac
      781 aatatttaca gactaccgaa aagccagttg atgaaattga ggcatctcag ac
//
LOCUS       DAZUCC010000555          828 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZUCC010000555 DAZUCC010000000
VERSION     DAZUCC010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.558"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..737
                     /gene="ydeO"
                     /locus_tag="ACHY09_005411"
     CDS             3..737
                     /gene="ydeO"
                     /locus_tag="ACHY09_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFS3888198.1"
                     /translation="MHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGF
                     NENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLL
                     FSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKK
                     KLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSP
                     KRVSKEYRCQRHTGMNTDNTMSALAI"
BASE COUNT          253 a          165 c          167 g          243 t
ORIGIN      
        1 ttattcatca tgccttcaac gctaacattt tagataaaga ttacgccttc tctgacggcg
       61 agatcctgat ggtagataac gctgttcgta cgcattttga accttatgag cggcatttca
      121 aagaaatcgg atttaatgaa aataccatta aaaaatatct acaatgcact aacatccaga
      181 cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc aaatgtaccg actggattgc
      241 ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa tcatcataat ttttcagaac
      301 ttttgctttt ttcttgcctg tctatttttg ccacatgcaa aggtttcatt acactattaa
      361 ctaacggtgt gctatccgtt tccgggaaag tgagaaatat tgtcaacatg aagctggcgc
      421 acccatggaa gctgaaagat atttgtgact gcctgtacat tagtgaaagc ctgttgaaga
      481 aaaaactaaa gcaagagcaa acgacattct cacagattct tttagatgcc agaatgcagc
      541 acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa caatgtggtt
      601 atgcgagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac agtccgaaga
      661 gagtttctaa ggagtaccgt tgtcaaaggc acacgggtat gaacacggat aacacgatga
      721 gtgctttagc tatttgatta tttgctaacg agtagtcaac cacgcacgtt acgtagaaat
      781 taaaatgggg cagccattcc ctgccccgcg ttgtttttag gcgatata
//
LOCUS       DAZUCC010000556          825 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZUCC010000556 DAZUCC010000000
VERSION     DAZUCC010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.559"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            8..>825
                     /locus_tag="ACHY09_005412"
     CDS             8..>825
                     /locus_tag="ACHY09_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFS3888199.1"
                     /translation="MPCIGNAQEIKIDESWLHQSLNVIGRTDSRFGPRLTNDLYPEYT
                     VAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKLTGLNLAFG
                     PFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQWQNYGAA
                     NENEWDGYRFKIKYSIPLTHLFGGRLVYNSFTNFDFGSDLADKSHNNKRTSNAIASSH
                     ILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGTYTTIGYQF"
BASE COUNT          252 a          159 c          170 g          244 t
ORIGIN      
        1 tctattaata ccttgcattg gaaacgctca ggaaattaaa attgatgaaa gctggttaca
       61 tcaaagcttg aatgtcattg gtcgcacaga ctctcgcttt ggcccaagac tgactaacga
      121 cctctaccct gaatatactg tagcaggaag aaaagactgg tttgattttt atggttatgt
      181 tgatctacca aaattctttg gcgtcggcag tcactatgat gttgggatct gggatgaggg
      241 ctcaccacta tttacggaaa tagaacctcg gttttccatt gacaaattga ccggattaaa
      301 tcttgcgttc ggccctttta aagaatggtt cattgcaaac aactatgtct atgatatggg
      361 tgacaaccag tcatcccggc aaagtacatg gtatatgggg cttggtacag atatcgacac
      421 gggtctacca attaagcttt ctgccaatat atacgccaag tatcagtggc aaaactatgg
      481 tgccgctaat gaaaatgaat gggacggata tcgattcaaa ataaaatata gcattcctct
      541 tacccattta ttcggagggc gattggtata caatagtttt actaactttg attttggctc
      601 cgatcttgcg gacaaatcac acaataataa acgaaccagt aatgctattg cttcaagcca
      661 tatcctttcc ctgctatatg aacactggaa atttgcattt acactacgtt attttcacaa
      721 cggtggacaa tggaatgctg gggagaaagt taacttcgga gatggtccat ttgaattaaa
      781 aaacacagga tggggaacct atactactat tggttatcaa tttta
//
LOCUS       DAZUCC010000557          818 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZUCC010000557 DAZUCC010000000
VERSION     DAZUCC010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.560"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>818
                     /locus_tag="ACHY09_005413"
     CDS             <1..>818
                     /locus_tag="ACHY09_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HFS3888200.1"
                     /translation="RDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLRGYVK
                     PHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRWIGKN
                     NHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDRDILY
                     RMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEKLRTP
                     PEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAVG"
BASE COUNT          180 a          198 c          286 g          154 t
ORIGIN      
        1 cgggatacgg tggaggatgc gctgcaggac agttttgatg aatatgactc acatccctgg
       61 gtggtgcagt tcttctgtca ggatgaaaac gacgtggaca cgtatgttga ccagctcagg
      121 gggtacgtga aacctcatgc agaaggctcc tcatttacgg aggcctggct gagggaaatg
      181 gagcgccatc tgaaggggat tgcccgaccg gaaggtctgt tcagggatac gctggtcaca
      241 ggtcagccat ggcgtggtca gcagcggcgc acgaggatgg tgatttaccg gtggatcggg
      301 aaaaacaacc acgatccgat gccgccggtg gcaatgctga accaggtctg ttcgcgggtc
      361 gtgggggcgc tgggtggcgc aggcgtccgc tgtacacgga tgaacgggca gcaggtgcat
      421 ggctggctgc tgaggctgtt caatccccgg ccggaatggg tggacaggga tattctgtac
      481 cgtatggcat cccgtgcaga accgcaggag acaccggagg gaatgatgcc ggtgatgacc
      541 gattttgcag aaagcctctg gttcacccca ccggtatctg atccggaaaa cggcgtgtgg
      601 tggctggacg ggcttccgca tgccgctgtt gtggtggaga aactgcgcac accaccggag
      661 ccgggaacca tcacagggga acaggcccgg ggcgaaaaga cagtgaatgc cctgatggac
      721 acctttccgg agggaacggt gctgtgtatg acaattgtgg tccagccaca ggacacactg
      781 gaagagcgct tcacgaggtt gtcgaaaaat gcggtcgg
//
LOCUS       DAZUCC010000558          817 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZUCC010000558 DAZUCC010000000
VERSION     DAZUCC010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.561"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..>817
                     /gene="reiD"
                     /locus_tag="ACHY09_005414"
     CDS             76..>817
                     /gene="reiD"
                     /locus_tag="ACHY09_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator ReiD"
                     /protein_id="HFS3888201.1"
                     /translation="MAYVSQVSESNGPFVRFMHMHPRTVELILITEGKGEYFIGDRIY
                     PVRKGDLVIYNSEVVHDEYLESGRPVGTICCGINNIVCPGLRENALIPDDTVPVIPLY
                     HHYATIEKLMSSIFNVINQHTVEGPTMAQLLTRVLLKYVEGNVLMRTGNDITRRHKNL
                     LDSIKAYIDHNFYEPIRLDALATKFNVSPYYVSHEFKRRYGYSPMDYLIKRRLGEAQS
                     LLTTDEGGCEKITSIAYRVGFSNLSHFQN"
BASE COUNT          232 a          150 c          196 g          239 t
ORIGIN      
        1 gattcggata atggaggaaa aatcatgcgt ttttgcttct gtccttcaga acaagataat
       61 aagtcccggg ctcagttggc gtatgtcagt caggttagcg aaagcaatgg cccgtttgta
      121 cggttcatgc atatgcatcc caggaccgtg gagctaatcc ttatcaccga gggaaagggg
      181 gagtatttca tcggtgatcg catttaccca gtcagaaaag gtgatttggt tatttataac
      241 agcgaagtag tgcatgatga gtatcttgaa agcggacggc ctgtgggtac tatttgctgt
      301 gggataaaca atattgtgtg cccgggtctg cgggagaatg ctctgattcc agatgatact
      361 gtgcccgtaa ttccactata tcatcattat gcgacgatcg agaaattgat gagttcaata
      421 tttaatgtta taaatcagca tacagtagaa ggaccaacaa tggcgcagtt gctgaccagg
      481 gtgctgctta aatacgtcga gggaaatgta ttaatgcgca caggtaatga tataacacgg
      541 cgccataaaa atttactgga ttcaataaaa gcttatattg accataattt ctatgaaccc
      601 atccgtctgg atgctctggc gactaagttt aatgtcagcc cttactatgt ttctcatgaa
      661 tttaaacggc gctatggata ttcgccaatg gactatttga ttaaacgtcg gttaggggag
      721 gcacaatctt tacttaccac tgatgaaggt gggtgtgaaa aaattacctc aattgcctat
      781 cgtgttggtt tcagtaattt gagtcatttt cagaact
//
LOCUS       DAZUCC010000559          817 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZUCC010000559 DAZUCC010000000
VERSION     DAZUCC010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.562"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..>817
                     /locus_tag="ACHY09_005415"
     CDS             81..>817
                     /locus_tag="ACHY09_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3888202.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRP"
BASE COUNT          207 a          154 c          270 g          186 t
ORIGIN      
        1 gtcagaacac ccggggtaat ttctccgtcg gtgccggtgt gggctggcag tggtaacagg
       61 gaaaagcaag gagaaatgta atgttattta gtcgaatgat gatgacgggt gtgctgtccc
      121 tgatgcttgc aggatgtgga aatctgagtc atgtcacgga tgaaggaacg acggaagaag
      181 tggtatggcc ttctccggag aaggtgatgt ttaatcatga cggaacacag aaaggttcgt
      241 ggccactgcc agaaaagtta cgtcagataa aggcgggtat gaataaggac cagatttatc
      301 agctggcggg gagaccacac tttgctgaag ggatgtctgg tgtaagggaa tgggattacc
      361 tgttcaactt taccggaccg gagggggatt atacctgcca gtacaagata ctgtttgacc
      421 ggaacatgga tgcccgctct ttctactgga agccggaaaa ctgtctgccg gaggcagaga
      481 cagcagaata cagggagtca gtccccggtg atatattatt tgattttgat tcatggactc
      541 tgacagagag ggggcgtgat gcgctttccc gtgtggcaga gaagtttcag gcgggtcatg
      601 ttgtcggggt gaagatagaa ggatttacgg accatcttgg cgggcaggcg tataaccggc
      661 tcctgtcaga gaaacgggca gaatcagtca gggggtggtt tattgcccgg ggattcaggg
      721 ctgaaaggat acatgcaacg ggccgtggat atgatgcacc agtggtgttc tgtccggatg
      781 tggagggcga agcactgaag gaatgtctgc gccccaa
//
LOCUS       DAZUCC010000560          815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZUCC010000560 DAZUCC010000000
VERSION     DAZUCC010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.563"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..804
                     /locus_tag="ACHY09_005416"
     CDS             <1..804
                     /locus_tag="ACHY09_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS3888203.1"
                     /translation="FGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERYTYIPTI
                     NIINKLREEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIILLNSHDG
                     SSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVPGVFDKVTENM
                     EAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRLEDKQNDLWTTWQRV
                     QENMIKGGLSGRSASGKNTRTRGITGIDGDIRINKALWMIAEQFREWKS"
BASE COUNT          241 a          145 c          211 g          218 t
ORIGIN      
        1 tttggtcggt ataattccat tcgccgtgag cgtcctttaa cggatgatga attaatgcag
       61 tttgtgcctt cggtattttc cggtgataaa catgaatccc ggagtgagcg ttatacatat
      121 attccaacaa tcaatatcat caataagtta cgtgaagaag gtttccagcc gttctttgca
      181 tgtcagagtc gggttcggga tttgggacgc cgcgaataca gtaaacatat gttacgtctt
      241 cgcagggaag ggcatattaa cgggcaggaa gttcctgaaa ttatcctgct taattcacat
      301 gatggttcat ccagttatca gatgatccca ggaatttttc gttttgtctg cacaaatggt
      361 ctggtgtgcg gaaataattt tggcgaaatc cgcgttccac ataaaggcga tattgtcggg
      421 caggttattg agggagcgta tgaagtgccc ggtgtctttg ataaggtcac tgaaaatatg
      481 gaggcgatga aagaaataca tcttaacagt gacgagcaac atttatttgg cagagctgca
      541 ctgatggtca ggtatgaaga tgaaaataaa acaccagtga cgcctgaaca aataattact
      601 ccccgtcgtt tggaggataa acagaacgat ctctggacaa cctggcagcg ggttcaggag
      661 aatatgataa aaggtggact gtcggggcga agtgcctccg gtaaaaatac caggacaaga
      721 ggcattacgg gtattgatgg tgatatccga atcaacaaag cattatggat gattgccgaa
      781 cagttcaggg agtggaagtc atgataatat aagta
//
LOCUS       DAZUCC010000561          811 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZUCC010000561 DAZUCC010000000
VERSION     DAZUCC010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.564"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..291)
                     /locus_tag="ACHY09_005417"
     CDS             complement(<1..291)
                     /locus_tag="ACHY09_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888204.1"
                     /translation="MHHYKFFALIAMLLCGASFASDVYFLCNTEKGTIKLDENKGVLR
                     YTLSKDHKTEFNYESKGSDYSGFKYNHYSRFQTDYFNVSFVNSEYKYTIFSNY"
     gene            complement(278..>811)
                     /locus_tag="ACHY09_005418"
     CDS             complement(278..>811)
                     /locus_tag="ACHY09_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase family protein"
                     /protein_id="HFS3888205.1"
                     /translation="AAKELGCEVAAIKAVAKTETGSYGSYFKFEDNDDYVPAILFERH
                     HFHKYTNGKYDQFEDISNPVAGGYGATSIQYAKLVKAYTLDKKAALKSASWGKFQILA
                     SNYATAGYASPEDFVFALSKSEKNQLKAFVSFIKADRVLLRSIRTKDWLSFAQRYNGP
                     RQKGYDLKMERNYNASL"
BASE COUNT          240 a          167 c          125 g          279 t
ORIGIN      
        1 ataattacta aatatcgtgt acttatactc tgagtttaca aacgatacat tgaaataatc
       61 ggtttgaaat cgtgagtaat ggttgtattt aaaccctgaa taatcactac ctttcgattc
      121 ataattaaat tcagttttgt ggtctttcga taaggtgtac cgaagtacac ctttgttttc
      181 atctagtttg attgttcctt tttccgtgtt gcataaaaaa tacacatcac ttgcaaacga
      241 tgccccacac agaagcattg caatcaacgc aaagaactta taatgatgca ttgtaatttc
      301 tctccatttt caaatcatat cctttctgcc ttggtccgtt atatctttga gcaaatgaaa
      361 gccaatcttt agtccgaata gagcgcagta atactctatc agccttaatg aagctaacga
      421 atgcttttaa ctgatttttc tcagacttac ttaatgcaaa aacgaaatcc tcagggctcg
      481 cgtagcctgc tgtggcgtag ttcgacgcta aaatctgaaa cttaccccag gatgctgatt
      541 ttaaagctgc tttcttgtct aaagtatacg ctttaactag ctttgcatac tgtattgatg
      601 ttgcaccata cccacccgca acagggtttg atatatcttc aaactgatca tacttcccat
      661 ttgtatattt atgaaaatga tgcctttcaa ataatatcgc tggaacataa tcatcgttat
      721 cttcaaattt aaaataggag ccataagaac cagtttctgt ttttgctaca gctttaattg
      781 ctgcaacttc gcatcctaat tcttttgctg c
//
LOCUS       DAZUCC010000562          800 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZUCC010000562 DAZUCC010000000
VERSION     DAZUCC010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.565"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>800
                     /locus_tag="ACHY09_005419"
     CDS             <1..>800
                     /locus_tag="ACHY09_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS3888206.1"
                     /translation="LDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICF
                     STAVQQLNTVLLVILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRF
                     QQARLAMQGVNDIVRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSL
                     CIRPGERVGIIGSIGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLL
                     EQNPRLFLGTLRENLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGL"
BASE COUNT          173 a          196 c          225 g          206 t
ORIGIN      
        1 ctggacggaa tagaaaccct gaagctgaat aatgcagtta actgggtcca gcgccgctgg
       61 gatggatata cagccagtgt cgcccgctcg tccatacggg taaaaaatac cagtaatgtc
      121 ataatctgct tttccacagc agttcagcaa ctgaatactg ttctgctggt cattctgggg
      181 acgtacctta ttcacagtga tgacccacat aaccgaatca ccatgggggc actgattgcc
      241 gcagttattc tttccggtcg tgcgctggca ccgcttggtc agatagcatc actggccacc
      301 cgttttcagc aggcccgcct ggccatgcag ggggtgaatg acattgtccg ccgtccggtg
      361 gagcgggaaa gtcagacaca ttatctgaca gtgaaggata tccagggaga gtttcgtttc
      421 agtgatgtct ccttttctta taagcccgga ttacccgacg cattgtgcgg tctgtcgctc
      481 tgtatccggc ccggagagcg ggtggggatt attggctcaa taggttgtgg taaatcgacg
      541 ctgctgaaaa tgatggtggg gctgtaccgt cagaacaggg gacaaattac tcttgacggg
      601 gttgcgatgc agcaactgga cccctgtttt gtccgggata aagtgtttct tctggaacag
      661 aatccccggc tttttctggg aactctgcgt gaaaatctgc agctttcgcg tctgccggga
      721 agcctgtcgg atgtggatat gattaatgca gccggacgtt tcgggtttga ccgttttatc
      781 cggcatcatc cccgcggact
//
LOCUS       DAZUCC010000563          799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZUCC010000563 DAZUCC010000000
VERSION     DAZUCC010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.566"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..633
                     /locus_tag="ACHY09_005420"
     CDS             <1..633
                     /locus_tag="ACHY09_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HFS3888207.1"
                     /translation="KSIGSKHMQFIELLETGTPNIDFSGHTENTFRIIDFSVPPTAYG
                     KFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYE
                     CDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRITK
                     VNNETISYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(599..721)
                     /locus_tag="ACHY09_005421"
     CDS             complement(599..721)
                     /locus_tag="ACHY09_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888208.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
BASE COUNT          241 a          160 c          157 g          241 t
ORIGIN      
        1 aaatctatcg gcagtaaaca tatgcaattt atcgaattgc tcgaaaccgg gacgccgaat
       61 attgatttca gtggtcatac tgagaataca ttccgtatca ttgatttttc tgtgcctccc
      121 acggcttatg gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt
      181 gaaattttca tccgtcagtt tgaaagcttt gtcagccgtt ttttggggaa tgggcatacc
      241 agttgtattt tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa tggagacatt
      301 tacgaatgcg accattttgt ctatccacag tacaaaattg gaaacattaa taaatcggaa
      361 ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc agcgaaatgt
      421 cagcaatgtg tatataaacc tatctgcaat ggtggttgcc ctaagcatcg tattactaaa
      481 gtaaacaatg agactatttc ctatttttgc gaaggttata aaatcctttt ttcaaccatg
      541 gtaccttata tgaacgccat ggtggagtta gccaagaaca gagtaccgct ttaccacatt
      601 atggatgttg caaaacaaat ggagaataat taataacgct ccttctttgc tccagtctta
      661 gcctccgtct tatgacctta acaaagcacc cgacataaat tcctgttggg tgtttcgtca
      721 tcatcatctt cccttgcgaa aattctggct tacgcggcat gcgtcagggc attagcaata
      781 cccttgatta tcaatagca
//
LOCUS       DAZUCC010000564          795 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZUCC010000564 DAZUCC010000000
VERSION     DAZUCC010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.567"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            327..>795
                     /gene="dolP"
                     /locus_tag="ACHY09_005422"
     CDS             327..>795
                     /gene="dolP"
                     /locus_tag="ACHY09_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFS3888209.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEV"
BASE COUNT          212 a          174 c          204 g          205 t
ORIGIN      
        1 agccgtgttt tctctgtttt atgaaaaagg caccgggggg ccacggtgcc ttttagcctg
       61 tgtataacga aaaggagatc ctgctcagca aaccaggtag ttaaaaaaca gaatctttac
      121 tacgctatca acgttttcgt gcaaaaacat gaacaatatt aaatttcagt acatccatat
      181 ccgtcataaa catttccgtc tggcagatac agatatgtca cgtaacgtat ccgctgtttc
      241 cggctgtgcc ggaagagaat aacagtccgt agcttgtttt gtttcactga aggtaaactt
      301 tttgtacgat aaattccggg ggagatatgc gtttattata ttctgctgtt gtcctgattt
      361 cagccttatt gttacaggga tgcgttgctg caactgtggt gggtacagcc gctgtcggga
      421 caaaggcagc aaccgaccca cgcagtgtcg gaacgcaggt ggatgatgaa gtgctggagc
      481 tgagggtcag cagtgcactt tcaaaagatg aacaaatcag aaaagaagca cgcatcagtg
      541 agattgcata tcagggacgc gtgctgctga tcggtcagtc tccggaccgt gaattatctg
      601 cccgtgcccg tcagatagca ctcggtgtcg atggtgttca ggaggtctat aatgaaatcc
      661 ggcaggggac tcccgtcagt ccggtcataa tggcaggtga tgcctggctc acgacaaaaa
      721 ttcgttctca gttactcaca agtgcccgtg tcaaatcatc cagtgtcaag gtggtgacag
      781 aaaacagcga agtct
//
LOCUS       DAZUCC010000565          795 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZUCC010000565 DAZUCC010000000
VERSION     DAZUCC010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.568"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..614
                     /locus_tag="ACHY09_005423"
     CDS             87..614
                     /locus_tag="ACHY09_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS3888210.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     KTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPLQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            672..>795
                     /locus_tag="ACHY09_005424"
     CDS             672..>795
                     /locus_tag="ACHY09_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFS3888211.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDNGEHF"
BASE COUNT          190 a          216 c          246 g          143 t
ORIGIN      
        1 aggagctttc gctcctctga aggggcacat gaacgccccg tctccggtat ccggtttatc
       61 tcacatcttt cagtcaggag gttctcatga gtgcacgtgg tatcaacaag gtcatcctcg
      121 tcgggcgtct gggcaatgat ccggaggtcc gttacatccc caacgggggc gcagtggcaa
      181 acctgcaggt ggccacgtca gaaagctggc gcgacaaaaa gacgggggag atgcgggagc
      241 agacggaatg gcaccgcgtg gtgctgttcg gcaagctcgc ggaagtggct ggtgaatatc
      301 tgcgcaaggg cgcgcaggtc tatatcgagg ggcaactccg aacccgtagc tgggaagata
      361 acggtatcac ccgttacgtc actgaaattc ttgttaagac cacgggcacc atgcagatgc
      421 tggggagtgc accactgcag aacgctcagg cacaaccgca gcctcagcag aatggtcagc
      481 cacagagtgc tgacgcgacg aaaaaaggtg gcgcgaaaac gaaaggccgt ggacgtaagg
      541 ccgcgcagcc cgagcctcag ccgcaaccgc cggaggggga ggattacggg ttttcagacg
      601 atatcccgtt ctgactggcc tgactgtgat aaccgccccg ccctgcgcgg ggcatcaccg
      661 gagatatgag gatgagcgaa tatttcagaa tacttcaggg actgcctgac ggtcctttta
      721 cccgcaaaca ggccgaagcc gttgccgcac agtaccggaa cgtctttatc gaggatgaca
      781 acggagaaca ttttc
//
LOCUS       DAZUCC010000566          787 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZUCC010000566 DAZUCC010000000
VERSION     DAZUCC010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.569"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            152..712
                     /gene="pagP"
                     /locus_tag="ACHY09_005425"
     CDS             152..712
                     /gene="pagP"
                     /locus_tag="ACHY09_005425"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFS3888212.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYDP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          228 a          142 c          180 g          237 t
ORIGIN      
        1 taatcgatgc gcggaaatat ttaacttaaa caagcggaaa taaatagagc agctattcag
       61 attattcttt atgttggggc tattaaggtt atgttaattg tagctttgct atgctagtag
      121 tagatttttg ataaatgttt tatggtcaca aatgaacgtg agtaaatatg tcgctatctt
      181 ttcctttgtt tttattcagt taatcagcgt tggtaaagtt tttgctaacg cagatgagtg
      241 gatgacaacg tttagagaaa atattgcaca aacctggcaa cagcctgaac attatgattt
      301 atatattcct gccatcacct ggcatgcacg tttcgcttac gacaaagaaa aaaccgatcg
      361 ctataacgag cgaccgtggg gtggcggttt tggtcagtcg cgttgggatg aaaaaggaaa
      421 ctggcatggc ctgtatgcca tggcatttaa ggactcgtgg aacaaatggg aaccgattgc
      481 cggatacgga tgggaaagta cctggcgacc gctggcggat gaaaattttc atttaggact
      541 gggattcacc gctggcgtaa cggcacgcga taactggaat tacatccctc tcccggttct
      601 actgccattg gcctctgtgg gttatgatcc agtgactttt cagatgacct acattccggg
      661 tacctataac aatggcaatg tgtactttgc ctggatgcgc tttcagtttt gagacaaatg
      721 aaattttagt aaactcttta aaatcaatgg gtaaaataat taacatcaaa aataacgcga
      781 cttttat
//
LOCUS       DAZUCC010000567          786 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZUCC010000567 DAZUCC010000000
VERSION     DAZUCC010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.570"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>786
                     /locus_tag="ACHY09_005426"
     CDS             <1..>786
                     /locus_tag="ACHY09_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFS3888213.1"
                     /translation="CWFENKRIIAFCDETQTFSFEKGTNYADLQRIINPQFSFNLSVV
                     LRCPRAVTDYLIAVSPASYQITSPRNNEPDTLKELVVTDPLSTLLNEILQLKESSVLP
                     DEITVLVPTDLVKNSLQNDMRTLGVNIETVSRFRGMESSVVITLYSESMEDAQLFCAY
                     SRATTAFIAIYDSEKLAWREKNKFLHKLVNRKEVQRAINEAKHNSSSSVLMAGFFREG
                     ELKLSTVKLAWSKHFSSWLVEFDERNSPEEMWIDYLYSYLPYP"
BASE COUNT          245 a          132 c          151 g          258 t
ORIGIN      
        1 cttgttggtt cgaaaataaa aggattatcg cattttgtga tgaaactcaa acattttcct
       61 ttgaaaaagg aacaaattac gctgatcttc agcgaataat aaatccccaa ttttctttta
      121 acttatctgt tgtgttaaga tgtcctagag ccgttactga ttatttaata gctgtcagcc
      181 ctgcctcata ccaaattact tcccctagga ataacgaacc ggatacgcta aaagaattag
      241 ttgttacaga tccactctcc acattgctta atgaaatttt acaattgaaa gaaagttctg
      301 tactgcctga tgagattact gtccttgttc ctacggattt agtgaaaaat tccctccaga
      361 atgatatgag aactttgggg gtgaatattg aaacagtatc tcgttttcga ggtatggaat
      421 catctgtcgt gattacattg tattccgaaa gcatggagga tgcacagtta ttttgtgctt
      481 attcaagagc tacaactgct tttattgcaa tttatgattc ggagaaatta gcatggagag
      541 aaaaaaataa atttttgcat aaactggtta acagaaagga ggttcagaga gcaattaatg
      601 aagcaaagca taattcttcc tccagtgttc ttatggccgg ttttttccgc gaaggagagt
      661 taaaacttag tactgtaaaa ttagcatggt cgaagcactt ttcttcgtgg cttgttgaat
      721 ttgatgaaag aaattctcca gaagaaatgt ggatagatta tttgtattcc tatttgcctt
      781 acccga
//
LOCUS       DAZUCC010000568          786 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZUCC010000568 DAZUCC010000000
VERSION     DAZUCC010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.571"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..768
                     /gene="ompT"
                     /locus_tag="ACHY09_005427"
     CDS             <1..768
                     /gene="ompT"
                     /locus_tag="ACHY09_005427"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFS3888214.1"
                     /translation="DWKFNSAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDW
                     MDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTAR
                     GGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSG
                     WVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKK
                     GNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          253 a          162 c          175 g          196 t
ORIGIN      
        1 gactggaaat tcaatagcgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc
       61 cagatatcta tcggggctgc tggctggaca actctcggca gccgaggtgg caatatggtc
      121 gatcaggact ggatggattc cagtaacccc ggaacctgga cggatgaaag tagacaccct
      181 gatacacaac tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac
      241 gaacccaatt accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca
      301 gccagaggtg gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc
      361 ttcccgaatg gagaaagggc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc
      421 ttgactggaa gttatcgtta tgaagatttt gaactcggtg gcacatttaa atacagcggc
      481 tgggtggaat catctgataa cgatgaacac tatgacccgg gaaaaagaat cacttatcgc
      541 agtaaggtca aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtcaca
      601 cctaacgcaa aagtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat
      661 acttcacttt atgatcacaa taataacact tcagactaca gcaaaaatgg agcaggtata
      721 gaaaactata acttcatcac tactgctggt cttaagtaca cattttaaga acgccaacta
      781 aaattt
//
LOCUS       DAZUCC010000569          785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZUCC010000569 DAZUCC010000000
VERSION     DAZUCC010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.572"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(47..673)
                     /gene="upp"
                     /locus_tag="ACHY09_005428"
     CDS             complement(47..673)
                     /gene="upp"
                     /locus_tag="ACHY09_005428"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS3888215.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
BASE COUNT          175 a          210 c          181 g          219 t
ORIGIN      
        1 caaaaaaaag ccgactctta aagtcggctt taattatttt tattctttat ttcgtaccaa
       61 agattttgtc accggcatcg ccgaggcccg gaataatgta tccgtgctcg ttcagtccct
      121 ggtcaatcga tgcagtatac agttcgacgt ccgggtgcgc tttttccagc gcagcgatac
      181 cttctggcgc agcgaccagc accagaactt tgatgctgct gcagcccgct tttttcagca
      241 ggtcgatggt cgcgataacg gaaccaccgg ttgccagcat tgggtcaacg atcagcgcca
      301 tacgctcatc gatgttagaa accagtttct ggaagtacgg taccggctcc agcgtttctt
      361 cattacggta cataccgacg acactgatgc gcgcactcgg aacgttttcc agcacaccgt
      421 ccatcatccc aagacccgca cgcagaattg gcacaacggt aattttctta cctttgatct
      481 ggtcgatttc taccgggccg ttccagcctt cgatagtgac tttttccgtt tcgaggtcgg
      541 cggtcgcttc gtaagtcagc aggctaccca cttcggaagc gagttcgcga aagcgcttgg
      601 tgctgatatc ttgctcacgc atcagtccca gcttgtgttt gacgagtggg tgtttgactt
      661 ccacgatctt catactcttt ctcctttgag gggcagccac aaaaaaaatc gacggattat
      721 acctcctttc ttcaaggcgg caatattatt ttcgttgact ttagtcaaaa tgataacggt
      781 ttgag
//
LOCUS       DAZUCC010000570          783 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZUCC010000570 DAZUCC010000000
VERSION     DAZUCC010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.573"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>783)
                     /locus_tag="ACHY09_005429"
     CDS             complement(<1..>783)
                     /locus_tag="ACHY09_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS3888216.1"
                     /translation="ALIFQGHPVIHAGQTVSASQSDWENREFSLNNLNLNNADFSLSR
                     NAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAAGTSSYTGHITLEQ
                     KSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLNIHKGGALTAQGGLFTS
                     GSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSSVLKARDQASVVGDIIS
                     DKQATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAIH"
BASE COUNT          216 a          191 c          166 g          210 t
ORIGIN      
        1 atgaattgca ccgcggtatg ccgtattaaa accactcagt aatcctaccg cgagtccccg
       61 ggatgccttc tcagacaata tgccctcttt acctgattca gttccgaagc tgattgtggc
      121 ctgtttatca gaaataatat caccaacaac agatgcctgg tctctggctt tcagtactga
      181 tgaatcagac atcagtttaa atccgccatc agccatattg atggtcggta aaaaagcagc
      241 atcatctgaa ttcactggtg taccggtaag cagaagggaa gcgtcaccaa tatcaatgct
      301 tccacttgta aacagccctc cctgagcggt cagcgcacct cctttatgaa tattcaggga
      361 gctgtttata aatgacgatg catctgagaa caggactgaa gatgaagaaa cattgatatg
      421 actgtcttca gacgtaaccc cgccacgaaa attatcgcgg atatccagtg ctgatttttg
      481 ctccagagtg atatgccctg tataggagga tgttcctgcg gcagcagatt tcccttcaac
      541 actgatgaca tcattacctg ttccgtcatt tttatcagta aagactgtat ctccgccgat
      601 aataacagtg ctctggttaa cggccctgat attcccgttc ataaatgcat tacgggacag
      661 actgaagtcc gcattattaa gattcagatt gttgagtgag aactccctgt tctcccagtc
      721 actctgcgat gcagaaacag tttgccctgc atgaataacc gggtgtccct ggaagataag
      781 agc
//
LOCUS       DAZUCC010000571          781 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZUCC010000571 DAZUCC010000000
VERSION     DAZUCC010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.574"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..757
                     /locus_tag="ACHY09_005430"
     CDS             41..757
                     /locus_tag="ACHY09_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS3888217.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          261 a          134 c          167 g          219 t
ORIGIN      
        1 ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa
       61 tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg
      121 tgtagaaatc tatgctgctg ccaaacaagg tgaacccgat ccagaattaa acacatcttt
      181 aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc
      241 aattgataaa gccaaaggcg gcggagatga aacgttcgta cagggacgtt atgaaggctt
      301 tggtcctaat ggctcaatga ttattgctga gacattgact tcaaatgtta accgtacgat
      361 tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt
      421 cagctatatg tttgacaata cgggtgtgat tgtatttaaa ggaacagacc ctgaccatat
      481 ttttgaaatt ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa
      541 cattgttatt tatactgaac ctaccgacct tcataaagga atcgcggctc taaaagcagc
      601 tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct
      661 ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga
      721 tgtacaaaaa gtttatcata acgtcgcaaa tctctaatga tattttaaag aaatctgtct
      781 t
//
LOCUS       DAZUCC010000572          780 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZUCC010000572 DAZUCC010000000
VERSION     DAZUCC010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.575"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(25..699)
                     /gene="tnpA"
                     /locus_tag="ACHY09_005431"
     CDS             complement(25..699)
                     /gene="tnpA"
                     /locus_tag="ACHY09_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFS3888218.1"
                     /translation="MNSQTAKDITGFRSYLPDALRLRFDEQLSVRAIALQLGLSHSTI
                     HNLFQRFNASGITWPLPESLSVAQLDAILYANRKKELPDDPQISESTWRKERRTSYSR
                     EFKIRLVKQALQPGAVVARIAREHNINDNLLFNWKRLYEEGLLNDEDLQECQAVPVAI
                     TETLDPVKPVTNPFWRNKSDESQNDTTVGSPQCELHLKSGVVKLFSPLTPELLRVLIR
                     EMKGGI"
BASE COUNT          196 a          175 c          194 g          215 t
ORIGIN      
        1 tggtaccggt cggcagcgtt atcatcagat cccccctttc atttcgcgaa tgagcaccct
       61 aagaagttcc ggagtgaggg gggaaaacag ttttaccaca cctgatttaa gatgcagctc
      121 acactgtggc gagcctacgg tagtgtcatt ctggctttca tcagacttgt ttcgccagaa
      181 aggatttgtc accggtttga cggggtcaag agtctcagtg attgccaccg gaacggcctg
      241 gcattcctgg agatcctcat cattcagtaa gccctcttca tacagtcttt tccagttaaa
      301 caacagattg tcattaatat tgtgctctct ggcgatccgt gcaacgactg caccaggctg
      361 taatgcctgt tttaccagcc tgattttaaa ttcacgactg taggaggttc gtcgttcttt
      421 tcgccatgtg ctttcgctga tttgaggatc atcaggtaat tccttctttc tattggcata
      481 aagtatggca tcgagttggg cgactgaaag tgattcaggt aatggccaag taataccgga
      541 ggcattaaat cgttgaaaga gattatgaat ggtggaatga ctgagaccta actgcagggc
      601 gatagcccgg acagataatt gctcgtcgaa tcttagacgt aatgcgtctg gcaaataaga
      661 gcgaaagccg gtgatatctt ttgctgtctg agaattcatc gttcgtgtcc atcaatatag
      721 atggacgcga ttgttgccag acaggacaac catcacaaga cgtcacaggc ggagcgctta
//
LOCUS       DAZUCC010000573          774 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZUCC010000573 DAZUCC010000000
VERSION     DAZUCC010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.576"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..161
                     /locus_tag="ACHY09_005432"
     CDS             30..161
                     /locus_tag="ACHY09_005432"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFS3888219.1"
                     /translation="MTYENNKKTHQQHIEILTAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(197..>774)
                     /locus_tag="ACHY09_005433"
     CDS             complement(197..>774)
                     /locus_tag="ACHY09_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3888220.1"
                     /translation="AEEENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFSAGGH
                     LASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPDSRKQLLGDNST
                     SENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKG
                     GHGFSVQWATDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT          271 a          164 c          126 g          213 t
ORIGIN      
        1 taatggccaa acctctggaa aagaataaaa tgacatatga aaataacaaa aaaacccatc
       61 agcagcatat tgaaatactt acggccataa aagagcatga ttgtgataaa gctgaacagc
      121 taatgtataa tcacctttat aaacgaattg aagcatcata gttactcgac gcttcaccag
      181 gtagtcacca taaatattat ttaaatccat gagaatacat ccagtccata aacagtttta
      241 tccatcctcc aacaggaaga tctgtggccc actgaacact aaagccatgc cctcctttct
      301 catagagatg catttcagca gaaacgcctt tctctattag gccacggtac atgagcagac
      361 tgttttcaat tgacacaaga tcatcatcac aacaatggag taaaaacaca ggaggaaaac
      421 atgatgtgac attaagatcc gcactatatt gtattatatt ttctgaggta ctattgtccc
      481 ccagtaattg cttcctggag tctggatgtg tcacatcatc tttcattgat attacaggat
      541 aaattaaccc gacaaaatcg ggaatagcga aaatctcttg ttctttactg tcagaagaac
      601 gtttgaaatc acaaatctga ttcacaaacg ttgaggcgag atgcccccca gcagaaaatc
      661 ctaatacacc aatacgatga cgctgtagat gccatttatc agcattctta cgaatataaa
      721 gcattgcaga tattgcatca ataaaagttt tgtttatatt ttcttcctca gctg
//
LOCUS       DAZUCC010000574          774 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZUCC010000574 DAZUCC010000000
VERSION     DAZUCC010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.577"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            197..>774
                     /locus_tag="ACHY09_005434"
     CDS             197..>774
                     /locus_tag="ACHY09_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888221.1"
                     /translation="MKMIFTGKVSGEKTVLAAGDRHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLAEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKSTAEGE
                     QKPVQPEGGQEAEKTEVSSTGSIEPGSGSSSD"
BASE COUNT          227 a          131 c          234 g          182 t
ORIGIN      
        1 tgtatgtgat ttgaggctga agataataca aatgttatat ctgtcagggg aaaataaatc
       61 cgaaaaaacg gaatgattaa ggaggggcag ccagagaaaa taaattatct gtaatctgat
      121 tcgtttttat ttaatgactc cctttagcgg ataaaacata agagcatact ggttttatta
      181 acctgaggat acttatatga aaatgatttt caccggcaag gtcagtggag aaaaaacagt
      241 actggcagcc ggagaccgtc atactgtaaa ggcacaggcc ggggaacagt atggactggt
      301 tgatgaggtg acaggtctgg tcccggatgg tgtggaagcg gaccgttcag gcgatgattt
      361 aattctgcgt aaaaaggaag atgatacaga aatccggatt gagggattct gggaagaatg
      421 ccagccgggt gaaacacagt gtacagccgt gtttaatgtt gtgggtgaaa atggccaagt
      481 aactgaagct gtcctgaccc aggatggccc tgtactggag aattttactg cagggcaaag
      541 tggcacactg gctgaaggta ttaataacgg gattatctgg ggggggattg cttttggtgc
      601 agggctggct gctattgcac tggctgccgg aggcggtggt ggaagcagtc gtcataagag
      661 tacagctgag ggtgagcaga agccggttca gcctgaaggc ggtcaggagg ctgaaaaaac
      721 agaagtatcc tctacaggct caattgagcc gggaagtgga agctcttccg acca
//
LOCUS       DAZUCC010000575          772 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZUCC010000575 DAZUCC010000000
VERSION     DAZUCC010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.578"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>772
                     /gene="csa"
                     /locus_tag="ACHY09_005435"
     CDS             <1..>772
                     /gene="csa"
                     /locus_tag="ACHY09_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="HFS3888222.1"
                     /translation="GPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSGNGGGEHG
                     SQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPEAGGNSDVQGGYIPEN
                     PNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEKAKAGI
                     MEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQFHYLV
                     VLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPE"
BASE COUNT          232 a          111 c          225 g          204 t
ORIGIN      
        1 ggcccaactg ctggtgagtc tatgggggga acaggggcga atctgaatca gcaaggtgga
       61 aataataata gtaatagtgg ggttcactgg ggtggtggtt caggctctgg taatggaggt
      121 ggtgaacatg gaagtcagac ggggtgggga tggagtaaaa ccaataatcc ggatgtgccg
      181 ccttatgttg atgataatgg gcaggtgcgc attaccatta caaatggtct ggtaaaaacg
      241 cctgtatatg gagttcctga ggctggcgga aatagtgatg ttcagggggg ctatattcct
      301 gaaaatccta atgatgaagt tgctcgtaag tgggataaaa acaacttacc gcgagagatt
      361 gatgtgagta tcgatggttt taaatatcgt gtaacgctta atgataatgg tagagctatc
      421 ggtattcttc gaacaggtgt ccgtccttac gtagggagtg aaaaagctaa ggcgggtatt
      481 atggaaaaaa tcaatcataa aacacctgag gagatatatg aggcgttagg ttttaataaa
      541 gatgaatctc agcggcagga aaaagcaaaa caacaggctg aagatgcgtg ggatcgtttg
      601 cctcctaatg tgagaaaatt tgatgttgat gttgaacagt tccactattt agtggtgctt
      661 gatgattatg gtaatgtttt atctgtaact agaacagctg ttcgtcctta tgtagggagt
      721 gaaaaagcta aggctggtat tatggataaa gtcgaccata agaccccgga ag
//
LOCUS       DAZUCC010000576          768 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZUCC010000576 DAZUCC010000000
VERSION     DAZUCC010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.579"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            202..>768
                     /locus_tag="ACHY09_005436"
     CDS             202..>768
                     /locus_tag="ACHY09_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888223.1"
                     /translation="MSPDIISSAVNRFSGWPSNDPLKTDKESMTSPMTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKT"
BASE COUNT          243 a          153 c          143 g          229 t
ORIGIN      
        1 aagcccatta tgttattgtt tcattagtaa taatcactta aatgtatcga tgaaacaggt
       61 atgacttagc gggagtgatt gtttctgagt gaacagagac cactttcgcc tgataaatga
      121 aaaatgagcg atttttgcaa gttagttttt ctatagccga gtacgctcag cgcctcgctt
      181 gattacaatg aggttgctgc tatgagtcct gatattatca gttctgctgt caatcgcttc
      241 tcaggttggc catctaatga tcctctaaaa acagataaag aatcaatgac atcacccatg
      301 actgtgcagg taataaagat gattgccgcc atccttgacc cacatcggat agtcgttgga
      361 atcgatacac tttatacaac gcagcgcgaa acatttacct cagagagtca acagctcatt
      421 gaatcagcat tgcaaacact tcatgtgatg aatttaaata acctggaaac aaatctcgat
      481 gaggaaggtc actatctggc ttttggatat aacgttgaca ctcctgacag aataaccgtt
      541 tctgcttata atgaaaaaaa gcctggctgc tatggccaat gtcttttaat acaggatgac
      601 gaaacagact tatttaacct gataaaagtt aaaatttttg aagccaggca taattatgtc
      661 gctcaattga tgagcgatcc ggaatttatg ttcaaattta gttatagcgc tcaacaaaat
      721 agattagaac ctcatcttct ggttcccact tatttccctc taaaaaca
//
LOCUS       DAZUCC010000577          762 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZUCC010000577 DAZUCC010000000
VERSION     DAZUCC010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.580"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..721
                     /locus_tag="ACHY09_005437"
     CDS             <1..721
                     /locus_tag="ACHY09_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS3888224.1"
                     /translation="DLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKP
                     LTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPY
                     LLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQT
                     ITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKH
                     LFAHGEYIKGITSLIKYKLKK"
BASE COUNT          244 a          145 c          145 g          228 t
ORIGIN      
        1 tgatctgccg acgtcacagt tttggtcgta cgcaacatac ttcagggtat tgtcttttga
       61 atatctgagt gaaagtattt ctacactgct gtatctggat gccgatgttg tttgtaaagg
      121 aagcctgaaa cctctcacag aaattatatt taaagatgag tttgctgcgg tcattcctga
      181 caatgatagt actcaggcgg catgtgcaaa acgcctcaac attcccgaaa tgaatggacg
      241 ttatttcaat gcaggcgtta tctatgtcaa tcttaaaaaa tggcatgaag caaatttgac
      301 accgtattta ctcacgcttt tacgagggga aactaaatat ggctctctta aatatttaga
      361 tcaggatgcg ttgaatatcg catttaatat gaataatatc tacctcgcga aggattttga
      421 tactatttat accctgaaaa acgaacttca tgatcgtagt catcgaaagt atcagcaaac
      481 cattaccgat aaaacagtat tgattcacta tacagggata actaaaccat ggcatagctg
      541 ggctggatat ccatctgcat catactttaa tatcgcgcgt gaacaatctc cctggaagaa
      601 atatcctctt aaagaggcgc ggactgttgc agaaatgcag aaacaatata agcatctgtt
      661 tgcccatggt gagtatatta aaggtataac ttcattaatt aagtacaagc ttaagaaata
      721 aattactctt atgattacaa gtatacgcta tcgcggcttc tc
//
LOCUS       DAZUCC010000578          760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZUCC010000578 DAZUCC010000000
VERSION     DAZUCC010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.581"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            170..616
                     /locus_tag="ACHY09_005438"
     CDS             170..616
                     /locus_tag="ACHY09_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888225.1"
                     /translation="MSEGTRVNDFAYVKQALLAVFHRMNTRPLTRLCEKDDIQRGIAD
                     IQWMLHHHYYPSPGQVGFIIFLLRDEKFRVVREDGRQSFLAVEIQSLIDVLKDIRKYL
                     QFVTRYDCDGCIIRLHASAERKYLWIFLECVIVFILCAVLFFLIIC"
     gene            672..760
                     /locus_tag="ACHY09_005439"
                     /pseudo
     CDS             672..760
                     /locus_tag="ACHY09_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="traU family protein"
BASE COUNT          220 a          136 c          195 g          209 t
ORIGIN      
        1 acaaaataaa gccagccggg aatggtcttc tcagaactct gaaaaagaga ctgttattta
       61 tgaagcgaaa acgaagcagg accgttaaca acagcgataa gaatatcagt attatgacca
      121 tttgcactcc gaaactgata catgaacaga tgtttaaggg tgatattaaa tgagtgaagg
      181 gacaagagta aatgattttg cttatgtcaa acaggccctt ttggcggtgt ttcatcggat
      241 gaatacccgg ccgctcacca ggctatgtga aaaagatgat attcagcgtg gaattgcaga
      301 tattcaatgg atgttacatc atcactatta tccatcaccc ggacaggttg gttttataat
      361 atttttattg cgggatgaga agttccgggt agtgcgggaa gatgggcgtc agtcattcct
      421 tgcagttgaa attcagtcgt taattgatgt tctgaaagat atacggaaat acctgcagtt
      481 tgtgacacgg tatgactgtg atggttgtat tatcaggctg catgccagtg ctgaacgtaa
      541 atacctgtgg attttccttg agtgtgtaat tgtgtttatt ttgtgtgctg tgctgttttt
      601 tctgattatc tgttaaaaac acctggtggt gaacatgtac ccggacagcg gacagcggac
      661 agcggacagc ggacagcgga cagtgccatc cgttcgggcg cagtgtgatg cgccgggaga
      721 cggaaaaaaa tccgcctgac acaaagaaaa acttcggtta
//
LOCUS       DAZUCC010000579          760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZUCC010000579 DAZUCC010000000
VERSION     DAZUCC010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.582"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..676
                     /locus_tag="ACHY09_005440"
     CDS             <1..676
                     /locus_tag="ACHY09_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888226.1"
                     /translation="PWSLLEPKSFLTEKKHDSIDKNDASVSVKKVGLSEGDALKNTGV
                     DKQEDDYSRHVGTEESKSKVSGTEQNKNKGKTPPKVDRNVLVPSYVKFNFRGHKKCSR
                     IFNELKSHLTFDNTPNAISILLRIFIDLSVSTFVEENKLQFKEPHRTPGLHDKVKMCA
                     NFLRENKKMTGSQCTAVITFSSQITKHDGSLQQYVHNPHLIPSKEAVNTEWDNFELLL
                     SLIWSE"
BASE COUNT          267 a          114 c          160 g          219 t
ORIGIN      
        1 gccgtggagt ttactggagc ctaaatcttt tcttacagag aaaaaacatg attctattga
       61 caaaaatgat gcatctgtga gtgtaaagaa agtaggtctt tcagaaggtg atgccttaaa
      121 gaatacaggt gtggataaac aggaagatga ttattctaga catgtaggca cagaagagag
      181 taaatctaaa gtttcgggta cggaacagaa taaaaacaag ggaaaaactc ctcctaaagt
      241 agatcgtaat gtgttggtgc catcttatgt gaaatttaat tttagagggc ataagaaatg
      301 ctctagaata tttaatgagc ttaagtcgca tctgacattt gataacaccc ctaatgctat
      361 ctcaatattg ttgagaatat ttatagatct ctctgtaagc acttttgttg aagaaaacaa
      421 gttacaattt aaggaaccac atagaactcc agggcttcat gataaggtta aaatgtgtgc
      481 taatttcctt cgggaaaata aaaagatgac ggggagtcaa tgtacggctg taataacttt
      541 ctcgagccaa ataactaaac atgatggttc attgcagcag tatgtacata acccacactt
      601 aattccctca aaagaggctg tcaacactga gtgggataac tttgagttgt tattaagcct
      661 tatttggagt gagtgagatt tgtctacatc tggacatgga tgtccagaaa ctgataaact
      721 acaaaatcag gaatgagagg gatttatgaa attttatact
//
LOCUS       DAZUCC010000580          760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZUCC010000580 DAZUCC010000000
VERSION     DAZUCC010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.583"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          212 a          188 c          158 g          202 t
ORIGIN      
        1 gtggaatatc gggaaaacag gaagttacgt taaaagccga aggaaaaact cacaatccct
       61 tcttcaatca gcctctgaaa atcggctttt cagcatgtct tcccccattg ttcgtatacc
      121 ctgttcgatg gattattatt gatccatatg atcgcttcac gtaaacacga ttctggctcc
      181 acaccattct ggacgcgatt gttaccaaaa aagacaacca tcacaagacg tcacagacgg
      241 tgcgtgcgct tactcctgta tatctggcgc aacagaatct accagtgtaa actctctatg
      301 cttttgcgca gaagcagtgg gcctgggttc gcttatgtcc tgcatctaca agcgaatgac
      361 tgaccagaga ctcccatatc tggtcagtcg cccattttta ttttccctat acactcagac
      421 tacaatagcc gtaacaaaca gcataccccc agtcatagtg gcctctttat cttcttatgg
      481 cttgtctgtg gtactggaga tagagcaact gtgggagtac tgttatggcc cccggtaaga
      541 aaaactcttg cttaatgtac agcagaactg ttcccagaaa gaagcctgtg catattttca
      601 tagaaggaga tcacccataa cggcgttttt cacggtaata cacgccaggt aaagaatgct
      661 ggattatcaa tcaaagctcc gcacaggaag cgcttatgcc gtagagcgcc ctctggagaa
      721 aagtgctact tcttgtcgat tcagactagt tcaggaaatt
//
LOCUS       DAZUCC010000581          755 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZUCC010000581 DAZUCC010000000
VERSION     DAZUCC010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.584"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>755
                     /locus_tag="ACHY09_005441"
     CDS             <1..>755
                     /locus_tag="ACHY09_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3888227.1"
                     /translation="NVTFIGRPVGADTAGIPSGDLPPVLQRALDKFTRDITASAREGK
                     IDPVSGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRP
                     VTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGG
                     ADAANLLKPALARGELRTIAATTWSEYKQYLERDAALERRFQMVRVDEPDDETACLML
                     RSLKSRYAEHHNVHITDEAVRAAVTLSRRYLTER"
BASE COUNT          155 a          212 c          238 g          150 t
ORIGIN      
        1 aatgtgacct ttatcgggcg tcccgtcggt gcggatacgg cgggtatacc gtcaggtgac
       61 ctgccgcctg tgttacagcg tgctctggat aaattcaccc gggacatcac ggccagcgcg
      121 agagagggga aaattgaccc ggtatcggga cgcgatacgg aaatccgtca gatggtggat
      181 attctctccc gccgccgcaa gaacaacccg attctggtgg gggaccccgg tgtggggaaa
      241 acggctctgg tggaagggct cgccctgcgt attgcggaag gtaacgtacc ggaatcactc
      301 agacccgtca ccctgcgcac ccttgacctc ggcctgctgc aggccggtgc cggcgtgaag
      361 ggtgaatttg aacagcgcct gaaaaacgtg attgatgccg tgcagcagtc accggctcct
      421 gtactgttgt ttatagatga agcccatacc cttatcgggg ccggtaatca ggccggcggc
      481 gcggatgcgg ccaacctgct gaagcctgcg ctggcgcggg gtgaactgcg taccattgcc
      541 gccaccacct ggtctgagta caaacaatac ctggaacgtg acgcggctct ggagcggcgt
      601 tttcagatgg tcagagtgga cgagccggat gatgagacgg cctgtctgat gctgcgctcc
      661 ctgaaatccc gttatgcgga acatcataac gtgcatatca ccgatgaggc ggtacgtgct
      721 gccgtcacac tgtcgcgccg ttatctgaca gaacg
//
LOCUS       DAZUCC010000582          755 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZUCC010000582 DAZUCC010000000
VERSION     DAZUCC010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.585"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>755
                     /locus_tag="ACHY09_005442"
     CDS             <1..>755
                     /locus_tag="ACHY09_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3888228.1"
                     /translation="NVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGK
                     IDPVSGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLSP
                     VTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSSAPVLLFIDEAHTLIGAGNQAGG
                     ADAANLLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVRVDEPDDETACLML
                     RSLKSRYAEHHNVHITDEAVRAAVTLSRRYLTER"
BASE COUNT          154 a          208 c          239 g          154 t
ORIGIN      
        1 aatgtgacct ttatcgggcg tcccgtgggt gcggatacgg cgggtatacc gtcaggtgac
       61 ctgccgcctg tgttacagag tgctctggat aaattcaccc gggacatcac ggccagcgcg
      121 agagagggga aaattgaccc ggtatcggga cgcgatacgg aaatccgtca gatggtggat
      181 attctctccc gccgccgcaa gaacaacccg attctggtgg gggaccctgg tgtggggaaa
      241 acggctctgg tggaagggct cgccctgcgt attgcggaag gtaacgtacc ggaatcactc
      301 agccccgtca ccctgcgcac ccttgacctc ggcctgctgc aggccggtgc cggcgtgaag
      361 ggtgaatttg aacagcgcct gaaaaacgtg attgatgccg tgcagcagtc atcggctcct
      421 gtactgttgt ttatagatga agcccatacc cttatcgggg ccggtaatca ggccggcggc
      481 gcggatgcgg ccaacctgct gaagcctgcg ctggcgcggg gtgaactgcg taccattgcc
      541 gccaccacct ggtctgagta caaacaatac tttgaacgtg acgcggctct ggagcggcgt
      601 tttcagatgg tcagagtgga cgagccggat gatgagacgg cctgtctgat gctgcgctcc
      661 ctgaaatccc gttatgcgga acatcataac gtgcatatca ccgatgaggc ggtacgtgct
      721 gccgtcacgc tgtcgcgccg ttatctgaca gaacg
//
LOCUS       DAZUCC010000583          755 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZUCC010000583 DAZUCC010000000
VERSION     DAZUCC010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.586"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>755
                     /locus_tag="ACHY09_005443"
     CDS             <1..>755
                     /locus_tag="ACHY09_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3888229.1"
                     /translation="ARECELTGELLESRSDISRQSETLHLQQALHDIQQNQPLLSVDV
                     DVRTVAGVVADWTGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQRLRAAKT
                     GLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLK
                     GSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGR
                     EIDFRNTVILMTSNLGSDLLMQQLSEKPETTES"
BASE COUNT          170 a          190 c          243 g          152 t
ORIGIN      
        1 acgcccggga gtgcgaactc accggtgagt tacttgaaag tcgcagcgat atttcccgtc
       61 agagtgagac actgcatctg cagcaggcac tgcacgacat tcagcagaat cagcccctgc
      121 tcagtgtgga tgttgacgta cgcaccgttg ccggtgtggt cgcggactgg accggggtgc
      181 cgttatcttc cctgatgaag gacgaacaga cggaactgct gcatctggaa aaggatatcg
      241 gcagacgggt ggtcggacag gacgtggcac tggaatccat tgcgcagcgt ctgcgtgcgg
      301 cgaaaacggg tctgacatcc ggtaacggcc cccagggggt gttcctgctg gttgggccca
      361 gtggtgtggg aaaaaccgaa acggcactgg cgctggcaga cgtgatgtac ggcggcgaaa
      421 aatcactgat taccatcaat ctgtcggaat accaggagcc ccatacggtt tcccagctta
      481 agggttcacc gcccgggtac gtgggatacg gtcagggcgg tattctgacg gaagctgtgc
      541 gtaaacgccc ttacagcgtg gtgctgctgg atgaagtgga aaaggcccac cgggatgtgc
      601 tgaatctgtt ctaccaggtg tttgaccggg gctttatgcg cgacggcgag ggacgtgaaa
      661 ttgacttccg taatacggtc attctgatga cctccaacct gggtagtgac cttctgatgc
      721 agcaactgag cgaaaagccg gagacaacgg aatcg
//
LOCUS       DAZUCC010000584          753 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZUCC010000584 DAZUCC010000000
VERSION     DAZUCC010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.587"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..434)
                     /locus_tag="ACHY09_005444"
     CDS             complement(60..434)
                     /locus_tag="ACHY09_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFS3888230.1"
                     /translation="MLRKSAPVSQPEANPKSPLLLNNPFQRENPMSEVKPLVTADAVK
                     DALRSEDVRGALKQKVRQTFEAQIEADVDAILDTILGEQPGEQPEPEPQPEDITSENG
                     DIQPESPVVDVTEAQPEPGTML"
     gene            complement(370..552)
                     /locus_tag="ACHY09_005445"
     CDS             complement(370..552)
                     /locus_tag="ACHY09_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFS3888231.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPITP"
BASE COUNT          163 a          207 c          165 g          218 t
ORIGIN      
        1 acggaagctc acaacatgaa aaaggccagc agtactttac tgatggccct gactcaccct
       61 tacagcatgg tgcccggttc gggttgtgct tcagtcacat caaccaccgg tgattccggc
      121 tgaatatcgc cgttttctga ggtgatatct tccggttgcg gttccggctc tggctgttcg
      181 cctggctgct ctcccagtat cgtatccaga atcgcatcca catctgcttc tatctgcgct
      241 tcaaatgtct ggcggacttt ctgtttcagt gctccacgca catcttctga gcgcagcgcg
      301 tctttcactg catcagcagt gaccagaggt tttacttctg acataggatt ttctcgttga
      361 aaggggttat taaggagtaa tgggctcttc gggtttgctt ccggctgact gactggcgct
      421 gattttctca gcagcccttt tgtctatctg tctgcgccag aagtcacgca tggccctgta
      481 cccacccgaa aggagataca gcacacagac cgccgtacag aagtacaaca tcacctgatg
      541 aataaatgtc ataatttctt accgttatgg ttgacaatga gaactgtttt cattaaaaaa
      601 aacaaccgat gtacgaaagc atcttttctt tacattctcc attgggatta cctccgccag
      661 cttccattcc tgccgctggc ggcttttttt atcatgccgc gacgtccgcg ttgttcactt
      721 ccaccttcac actgtcaatc agcagcgtat atg
//
LOCUS       DAZUCC010000585          752 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZUCC010000585 DAZUCC010000000
VERSION     DAZUCC010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.588"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..236)
                     /locus_tag="ACHY09_005446"
     CDS             complement(<1..236)
                     /locus_tag="ACHY09_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HFS3888232.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKV"
     gene            complement(304..708)
                     /locus_tag="ACHY09_005447"
     CDS             complement(304..708)
                     /locus_tag="ACHY09_005447"
                     /inference="COORDINATES: protein motif:HMM:NF019154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HFS3888233.1"
                     /translation="MEKTAEFSLTEDSRVESEFIRWLKEGLREGSLSVNSVDSNVHVV
                     SGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFFQARLYSDAEHS
                     GRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
BASE COUNT          200 a          227 c          128 g          197 t
ORIGIN      
        1 accttgttat tccaggtcac ctccctgcgc ttctgctctt tcaggacata acgccggtat
       61 cgctgaacat cacggacagt cacctccccc ggcaaaatat caccgataaa ttccctgaat
      121 accctgacaa ctttgcagta gctccactct gtatccggcc gaagattctt tgaaaaaaag
      181 tactcttcca gcagttgttc ccaggtgatt tttgtgacgg aatcgtcatt ctgcattata
      241 tattcccctt gtttactgtg ctcccgcact ctccggtcaa attctgcaca tcatcgcctt
      301 tactcacccg aaaacaataa gcgggctgtc tccgggaacc gtaatattcc cgtacagaaa
      361 cctgccctta accagatatc ccgaaaccat tttgtaacgt ccggaatgtt ctgcgtcact
      421 ataaagacgg gcctgaaaaa aacgctctcc tgaacgtacc ttatggatac ctgcccgttc
      481 aaagagcgac tgaacataac gacgatcctg cccggaacca ctctccttca gaaaacgata
      541 aaaaatcccc ggggcacaca gaaaaacata tccggaaaca acatgaacat ttgaatcaac
      601 gctgttaacc gaaagtgaac cctccctcag cccctctttt aaccatctga taaattcact
      661 ctctaccctg ctatcctctg tcagggaaaa ctctgccgtt ttttccactg tttccgggat
      721 acggtttccc tgattttctg ttacacatcc gc
//
LOCUS       DAZUCC010000586          748 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZUCC010000586 DAZUCC010000000
VERSION     DAZUCC010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.589"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..114)
                     /gene="lfpB"
                     /locus_tag="ACHY09_005448"
                     /gene_synonym="stgB"
     CDS             complement(<1..114)
                     /gene="lfpB"
                     /locus_tag="ACHY09_005448"
                     /gene_synonym="stgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone protein"
                     /protein_id="HFS3888234.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAG"
     gene            complement(162..734)
                     /gene="lpfA-O113"
                     /locus_tag="ACHY09_005449"
     CDS             complement(162..734)
                     /gene="lpfA-O113"
                     /locus_tag="ACHY09_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFS3888235.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQTVPLGKVSKNAFSGVGSLASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          204 a          182 c          164 g          198 t
ORIGIN      
        1 gcctgcatag gttaatgctg gttggactaa aaaaacactg agtataatcg cggtaaataa
       61 cttacatgaa acggttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
      121 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
      181 gtaaattgtg aatcggcgtt cgccgtaccc gccgttacgg ttgcattggt tgccacatag
      241 cgtgcaataa acttcatttc agccttgcct tcagcgtcga tagcggtata ggcagaatcg
      301 ttataaagtt taacctgcga gttatctgac tggttaaaaa tacgaatacc gacacccgtt
      361 gccgcaatcg cttgtcctgt accggtaaaa tcgccagggt tgcccgctgt aagcggcgta
      421 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgcc
      481 tgagtgtagg ttgccgggca cttttcaagt ttaatactga actgctgtgg tgacgccaga
      541 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacggt ttgatcggct
      601 gtatcggagg taacttcaca agtcgtgtcg acaatttcac cgacgaagtg aacaacacca
      661 tcttctgcca gtgcatgtcc ggccagcatt aaagatgcca gagtgaatgc accgcctata
      721 atattacgct tcataaaaac tccattga
//
LOCUS       DAZUCC010000587          747 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZUCC010000587 DAZUCC010000000
VERSION     DAZUCC010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.590"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..260)
                     /locus_tag="ACHY09_005450"
     CDS             complement(<1..260)
                     /locus_tag="ACHY09_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888236.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTI"
     gene            complement(342..719)
                     /locus_tag="ACHY09_005451"
     CDS             complement(342..719)
                     /locus_tag="ACHY09_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888237.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
BASE COUNT          192 a          226 c          180 g          149 t
ORIGIN      
        1 tcaatagtga cattcaccga ccacagcccg tcggcagacc ggcagtaaaa cgccagcgtg
       61 tccgttaccg gaaacaactc tgacgcccgg gtcagggagc cgaaatacgc attctgcggc
      121 cgtaacgcct ccggcaactg ccagaaggtt tcagtgacag ccagaacaaa agcatgccac
      181 tgcttttccg gtattgtgga aacctgctca agggtatata ccaccggctc acgggccgga
      241 cgtgaagata actcagacat ggaacattcc tcatggttaa aacgaaacaa cggggaatgt
      301 ctcccggacg gagaactccc ccgtcagggt caggtcatga attacatcat gtcgtgccgg
      361 gcaaaatatt catccgctcc ggccaccatc tttccggtac tgatactgtc agcccagatt
      421 tcatgattaa tttcacgccc cagagccatc agactgtcga gtgcctgacg ggcctgaatt
      481 cccggtgcgg aaatctcctc ctgctccagg tcatacaaca gccagcccag cgcctcgccg
      541 gtatcccaca gatacacgta taccagtgaa ggctccgtct catcatccgg ctccggggta
      601 atacccagcc gttgccaggc acgtaaccgc atcagctcgc cttccatctg actgatatga
      661 gccagcagag cagactgcgt ctgaagcgct gaataatgag caattaaagg tgaatacatg
      721 atgacctccg tctcttaaag gaagtcc
//
LOCUS       DAZUCC010000588          740 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZUCC010000588 DAZUCC010000000
VERSION     DAZUCC010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.591"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..675)
                     /locus_tag="ACHY09_005452"
     CDS             complement(<1..675)
                     /locus_tag="ACHY09_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFS3888238.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSGNGAAAVIDSCQGYDIKANCQ
                     ASRQSLSGNMQDWSIADGQWLIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTI"
BASE COUNT          207 a          172 c          142 g          219 t
ORIGIN      
        1 tatcgttttt cctgaaagat gtgtgttatc cgtactatta tccgtaacat caatagcgcc
       61 gccatcccca tcactataac ttgttgatgt atatgcctgg ttattattaa aagcatcatc
      121 acttaaatag acgtcattat tgatggtata aattgcaccg cctttgccgt cattagcgat
      181 gttattgtga aatacggcgt tagtgacacg taaatcggcg gcaccggtat cattggtacc
      241 ggcagaatag attgctccgc catagcctcc tgcaacatta ctgtcgaaaa taacattcgc
      301 aagatttatc gttgagtttt ctttagcaaa gatagcccca ccattattat attcgccact
      361 aaccgagtta tcagcaaaca gggtcatccc tgtttcattt tgtggtgaaa tcgtaaattc
      421 agcactttgc tgcaaaaata ctgcgccacc gctggcatta ttggccatac ccgaaaaaat
      481 caaccattgc ccatccgcga tgctccagtc ctgcatattg cccgaaaggc tttgcctgct
      541 ggcctgacag ttcgctttga tatcatatcc ctggcatgaa tcgatgaccg ctgcggcacc
      601 gttaccagag aaaagagatg caatcattga cgggagtaaa gataaatact ccttgcgtag
      661 aaagataatc cgcatattca tctaaccatc attttctata agatggcgta tttaatcgca
      721 ttatgcgata tagggtattt
//
LOCUS       DAZUCC010000589          732 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZUCC010000589 DAZUCC010000000
VERSION     DAZUCC010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.592"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..694
                     /gene="fimE"
                     /locus_tag="ACHY09_005453"
     CDS             98..694
                     /gene="fimE"
                     /locus_tag="ACHY09_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFS3888239.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          198 a          140 c          178 g          216 t
ORIGIN      
        1 tgtgtggtta tctttttatc tattgggcta attttgaccg attgaggttt cctataggta
       61 ttcattcaaa tatatctcag ttaggagtac tactattgtg agtaaacgtc gttatcttac
      121 cggtaaagaa gttcaggcca tgatgcaggc ggtttgttac ggggcaacgg gagccagaga
      181 ttattgtctt attctgttgg catatcggca tgggatgcgt attagtgaac tgcttgatct
      241 gcattatcag gatcttgacc ttaatgaagg tcgaataaat attcgccgac tgaagaacgg
      301 attttctacc gttcacccgt tacgttttga tgagcgtgaa gccgtggaac gctggaccca
      361 ggaacgtgct aactggaaag gcgctgaccg gaccgacgct atatttattt ctcgccgcgg
      421 gagtcggctt tctcgccagc aggcctatcg cattattcgc gatgcaggta ttgaagctgg
      481 aaccgtaacg cagactcatc ctcatatgtt aaggcatgct tgcggttatg aactggcgga
      541 gcgtggtgcc gatacccgtt taattcagga ttatctcggg catcgaaata ttcgccatac
      601 tgtacgttat accgccagta atgctgctcg ttttgccgga ttatgggaaa gaaataatct
      661 cataaacgaa aaattaaaaa gagaagaggt ttgatttaac tcattgataa taaagttaaa
      721 aaacaaataa at
//
LOCUS       DAZUCC010000590          726 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZUCC010000590 DAZUCC010000000
VERSION     DAZUCC010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.593"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..701
                     /locus_tag="ACHY09_005454"
     CDS             93..701
                     /locus_tag="ACHY09_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFS3888240.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
BASE COUNT          170 a          159 c          248 g          149 t
ORIGIN      
        1 gggtatgagg ccgggaaaaa tgcctcactg catcatggtg tgggggagag tgcccgacag
       61 gtggtgccgg gtctgatgat acgggaggcg tcatgaataa cagcttgtct caggcaacca
      121 acggtctgct gatgcaactg gtgatggacc tcaagagcgg ctacctgcgt cgctgcgaat
      181 ctctggggct ggcccgggag gaaatgcaga tgctgcaggg actgaccatt gaggaaattc
      241 attacctgtc aaactctgaa gtgtcggtta tccggctgga cattaatcat gaaaacctgc
      301 tgcgtatgct gcagcaggcc cggacggagc agaaacgtct gcagcgcatt gaccgggcac
      361 tggcgctggg aggctccatt gaactgatgg cgttttactt tggactttcc agtgtggatg
      421 tggcggcgcg ccgtcgcatt gccggtattg atgttcgccc gggacgtggc atcacactga
      481 gtgatgagga aagtgcagag ctgtggcgtt tatggcagaa gagcggcatc acggatgtgg
      541 agagtgcgga cggactggat gtcatgatgc tggcagctga gcaaatgaat atcccgctga
      601 cggcggtatg gcatgccgtg aggggatggc atcagggaac tgcggggttt aatgatgtat
      661 cggccagtcg ccggaaactg aaccgggaaa aagcagaatg acaaggacaa atgacatggc
      721 cgaaat
//
LOCUS       DAZUCC010000591          725 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZUCC010000591 DAZUCC010000000
VERSION     DAZUCC010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.594"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>725
                     /locus_tag="ACHY09_005455"
     CDS             <1..>725
                     /locus_tag="ACHY09_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFS3888241.1"
                     /translation="HLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKA
                     SSGNNDFRARGWGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFG
                     HGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSS
                     RGDMRVGTSTAGSGMTFSPSQNGT"
BASE COUNT          143 a          207 c          230 g          145 t
ORIGIN      
        1 catctcggtc acgataacaa cggcggtatt gcccgtgggg ccacgccgga aagcagcggc
       61 agctatggct tcgtccgtct ggagggtgac ctgctcagaa cagaggtcgc cggtatgtca
      121 ctgacgacag gagtgtatgg tgctgcaggc cattcttccg ttgatgttaa ggatgatgac
      181 ggctcccgcg ccggcacggt ccgggatgat gccggcagcc tgggcggata tatgaacctg
      241 acacacacct cctccggcct gtgggctgac attgtggcac agggaacccg ccacagtatg
      301 aaagcgtcat cgggcaataa cgacttccgc gcacggggct ggggctggct gggctcactg
      361 gaaaccggtc tgcccttcag tatcactgac aacctgatgc tggagccacg gctgcagtac
      421 acctggcagg ggctttcgct ggatgacggt aaggacaacg ccggttatgt gaagttcggg
      481 catggcagtg cacagcatgt gcgtgccggt ttccgtctgg gcagccacaa cgatatgacc
      541 tttggtgaag gcacctcatc ccgtgccccc ctgcgtgaca gtgcaaaaca cagtgtgcgt
      601 gaactgccgg tgaactggtg ggtacagcct tccgttatcc gcaccttcag ctcccgggga
      661 gatatgcgtg tggggacttc cactgcaggc agcgggatga cgttctctcc ctcacagaat
      721 ggcac
//
LOCUS       DAZUCC010000592          725 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZUCC010000592 DAZUCC010000000
VERSION     DAZUCC010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.595"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>725
                     /locus_tag="ACHY09_005456"
     CDS             <1..>725
                     /locus_tag="ACHY09_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFS3888242.1"
                     /translation="HLGHDNNGGIARGATPESSGSYGFVRLEGDLLITEVAGMSLTTG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKA
                     SSGNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFG
                     HGSAQHVRAGFRLGSHNDMNFGKGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSS
                     RGDMRVGTSTAGSGMTFSPSQNGT"
BASE COUNT          146 a          211 c          225 g          143 t
ORIGIN      
        1 catctcggtc acgataacaa cggcggtatt gcccgtgggg ccacgccgga aagcagcggc
       61 agctatggct tcgtccgtct ggagggtgac ctgctcataa cagaggtcgc cggtatgtca
      121 ctgacgactg gggtgtatgg tgctgcaggc cattcttccg ttgatgttaa ggatgatgac
      181 ggctcccgcg ccggcacggt ccgggatgat gccggcagcc tgggcggata tatgaacctg
      241 acacacacct cctccggcct gtgggctgac atagtggcac agggaacccg ccacagtatg
      301 aaagcgtcat cgggcaataa cgacttccgc gcacggggct ggggctggct gggctcactg
      361 gaaaccggtc tgcccttcag tatcactgac aacctgatgc tggagccaca actgcagtac
      421 acctggcagg gactttccct ggatgacggc caggataacg ccggttatgt gaagttcggg
      481 catggcagtg cacaacatgt gcgtgccggc ttccgtctgg gcagccacaa cgatatgaac
      541 tttggtaaag gcacctcatc ccgtgccccc ctgcgtgaca gtgcaaaaca cagtgtgcgt
      601 gaactgccgg tgaactggtg ggtacagcct tccgttatcc gcaccttcag ctcccggggt
      661 gatatgcgtg tggggacctc caccgcaggc agcgggatga cgttctcacc gtcccagaat
      721 ggcac
//
LOCUS       DAZUCC010000593          725 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZUCC010000593 DAZUCC010000000
VERSION     DAZUCC010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.596"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>725)
                     /locus_tag="ACHY09_005457"
     CDS             complement(<1..>725)
                     /locus_tag="ACHY09_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFS3888243.1"
                     /translation="FGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINW
                     MRDCHSSDDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNE
                     ITAIHELLNMLDIKGKIITTDAMGCQKDTAEKIQKQGGDYLFAVKGNQGRLNKAFEEK
                     FPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFMFEWKGLKKLCVAVSFR
                     SIIAEQKKEPEMTVRYYISSADLT"
BASE COUNT          195 a          163 c          131 g          236 t
ORIGIN      
        1 gttaaatcag cagaactgat ataatatctg accgtcattt cgggctcttt cttttgttct
       61 gctattattg accgaaagga gactgccacg cataatttct tcagaccttt ccattcaaac
      121 atgaaatcaa taagttcatc agggacatcg caaacaatgt gaagacggat ttcttctctg
      181 ccgtgacttt tttcactaat tgcgtaactg tcatgctctg gattatttaa ttctttcagt
      241 gggaattttt cctcaaaggc tttattaagc cgcccctgat ttccttttac agcgaataaa
      301 taatcaccgc cctgtttttg tatcttctct gcggtatctt tctggcaacc catcgcatca
      361 gttgtgatga tttttccttt aatatccagc atgttaagaa gttcatggat agctgtgatc
      421 tcattggatt tctcatccgt cctgatctgt ccgatgacca gactgtgcat tgttgagaac
      481 gcactaatga catgaatcgc tcccctgcgg cgactcttgt cataagaatg ccggagcgtt
      541 tttccatcaa ttgcaataac gtctttatca tctgaagaat ggcagtcacg catccagtta
      601 ataaagcact cgtgaaattt tgcaggactg atacaggata caactctggc aatggtatcg
      661 tgaacaggaa taccattttc aaaatcacca tattgcttca aaaaatcgag atgtgtttcc
      721 ccaaa
//
LOCUS       DAZUCC010000594          721 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZUCC010000594 DAZUCC010000000
VERSION     DAZUCC010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.597"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>721
                     /gene="ehxD"
                     /locus_tag="ACHY09_005458"
     CDS             <1..>721
                     /gene="ehxD"
                     /locus_tag="ACHY09_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFS3888244.1"
                     /translation="SLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNE
                     HAVWLAQVSQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRK
                     ASSFIKAPVSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQAG
                     QEVVIKVDAYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGEIKKMP
                     VTAGMTVMAEIKTGVRSVISYL"
BASE COUNT          254 a          111 c          159 g          197 t
ORIGIN      
        1 ttagcctgta tgagcatcag gtatcacagg aaggaagaaa actcaatgat tttaagtact
       61 tgttgaataa aaaagctgtt tctcaacatt cagttatgga gcaggaaaat agctatattc
      121 aggcaaaaaa tgaacatgca gtctggcttg cacaggtttc tcaacttgaa aaagaaatag
      181 aacttgtgcg ggaagaactg gcactggaga cgaatatctt cagaagtgaa attatcgaga
      241 agcacagaaa atcaacagat aacattgtgt tgctggagca tgagcttgaa aaaaacaggc
      301 agagaaaagc atcatctttt attaaagctc ctgtgagtgg tactgttcag gagttaaata
      361 tacatacaga aggtggtgtt gtcacaacag cagaaacgct gatgattatt gtccctgata
      421 atgatattct cgaagtaaca gcatctgtac ttaacaagga tatcggtttt atacaagctg
      481 gacaggaggt cgttattaaa gtagatgcat atccatatac acgtcatggt taccttacag
      541 ggaaagtaaa aaatataact gcagattctg tttctgttcc cgatacagga cttgtattta
      601 acgtgattat atctgttgat cggaatgata ttcagggaga aataaaaaaa atgcctgtta
      661 cagctggaat gactgttatg gcagaaataa aaaccggagt gcgtagtgtt atcagctatc
      721 t
//
LOCUS       DAZUCC010000595          719 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZUCC010000595 DAZUCC010000000
VERSION     DAZUCC010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.598"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..381
                     /locus_tag="ACHY09_005459"
                     /pseudo
     CDS             26..381
                     /locus_tag="ACHY09_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(614..718)
                     /locus_tag="ACHY09_005460"
                     /pseudo
     CDS             complement(614..718)
                     /locus_tag="ACHY09_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
BASE COUNT          201 a          178 c          168 g          172 t
ORIGIN      
        1 gttatcttca atatgaaatc accgttaccg ctggcgcatc ctgacccaac ttaaccgggg
       61 agaaagtcgc catgccgttg ccagagctat ctgtcacggt cagaaaggtg agataagaaa
      121 gcgatatacc gatggtcaag aagatcaact gggcgcactg gggctggtga ctaacgccgt
      181 cgtattatgg aacactattt atatgcaggc tgccctggac catttcaggg cacaggggga
      241 aatcgtgaat gatgaagata tcgcacgcct ctcaccactt tgccacggac acatcaatat
      301 gctcggccat attccttcac gctggcagaa ctggtgacta aagggcaaaa agaggcatcg
      361 gaggaagaaa acattgctta acgtgagttt tcgttccact gagcgccagc cccctaattc
      421 tctatatcca aactgacccc aataggtgac gggttatgtg acgaaatctg atgtagaaat
      481 cgttgtttca gtgacagtca ctcaatcggt catttatgtg acaccccacg ccgttactgg
      541 tcgcggaaaa atccagcgga aaaatccagg tttccggctt gggaccccat ctcaagagat
      601 cctttgatct tttctactga accgcgatcc ccgtcagttc agaagacgaa gatgacgcaa
      661 cggttcctca ttgtacatca cgaatatagt ttgcttgaca tcctgacaag aataatatt
//
LOCUS       DAZUCC010000596          715 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZUCC010000596 DAZUCC010000000
VERSION     DAZUCC010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.599"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..206)
                     /locus_tag="ACHY09_005461"
     CDS             complement(<1..206)
                     /locus_tag="ACHY09_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888245.1"
                     /translation="MQYAEFQAEATANGIRTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVA"
     gene            complement(212..394)
                     /locus_tag="ACHY09_005462"
     CDS             complement(212..394)
                     /locus_tag="ACHY09_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888246.1"
                     /translation="MHQKTAEHEQTRVLLTIKNGKVIFIRHVHDDELVGSFSTFLFMV
                     KQAGYEVTPPADEDEE"
BASE COUNT          184 a          155 c          139 g          237 t
ORIGIN      
        1 gcaaccttca tctcaaccgg aagttttccc agtaatcctg cttcgtcagc ttgcgcgaga
       61 cactgaagga tacgtaaacc tcgcacatta ggagtatcga attctccagc atccagacgg
      121 cggattgcgt cgtgataatc aatagtcata ctgcctgttc gtataccatt ggctgttgct
      181 tcagcctgga attcagcgta ttgcatgaaa tttattcctc atcttcatca gctggtggag
      241 taacctcata tcctgcttgc tttaccatga acaggaatgt tgaaaaactt cctacaagtt
      301 catcgtcatg aacatggcga ataaatatta ctttcccgtt tttgatggtc agcaatactc
      361 tggtttgttc gtgttctgct gttttctgat gcattattat ctcccgtatg ctttacgcag
      421 aaacaagcat gcaatatgca tgtaattttc accgtattgt gcaataaggc aggcggtctt
      481 gtgtgatgcc atattcttta taaaagtcat aataaaacct cctgtggatt aagggtgtga
      541 taatccccgg cgattaagcc gtaatatatt attcggatat gacttgtttt atttaactgg
      601 atagttcttt ttcagctgct gcttcagcat agcaacgtgc aaattcaaga acttcatcac
      661 ctgttctttt tattgcgtcg ttgtctgaca tttgtaatac gacaactgcg cataa
//
LOCUS       DAZUCC010000597          712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZUCC010000597 DAZUCC010000000
VERSION     DAZUCC010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.600"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..189
                     /locus_tag="ACHY09_005463"
     CDS             <1..189
                     /locus_tag="ACHY09_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFS3888247.1"
                     /translation="NETAEWVKQRYGQTFDAIYVPPGQTLAVHITRQLAIDYEDQGRR
                     VRYDFTLPGEVHGTGGLD"
     gene            225..617
                     /locus_tag="ACHY09_005464"
     CDS             225..617
                     /locus_tag="ACHY09_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFS3888248.1"
                     /translation="MTVMVAVFILSGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
BASE COUNT          168 a          174 c          221 g          149 t
ORIGIN      
        1 aatgaaaccg cggaatgggt gaaacagcga tacgggcaga cgtttgatgc catttatgtt
       61 cccccggggc agacgctggc ggtgcatatc acgcgtcaac tggccattga ttatgaagat
      121 caggggcgcc gggtacgtta cgacttcact ctgccggggg aagtccacgg taccggtggt
      181 ctggactgat gtaaggagat cccgtgaaac gacagttatc tgtaatgacc gtcatggtgg
      241 cggtatttat tctgtccggc tgctcgacat cgaaagagga gcttctgcct ccgggtgaac
      301 agagtatgct ggagatctgg caggggaaag gaagcggagg acattcgcgg cacatgacag
      361 cggccaggga caccctgcgg cgtccgctga caatgccgga gcagcaggca gatgcgcacg
      421 gagccataag ctacagccgg acacaggaaa gtgagatcag tcagcagttt ccgcggctgc
      481 ccaatcctga catggtgatg tatgtttttc cgcatctggc ttccggtacg tcaccggttc
      541 cggggtacag cacagtattt cctttttaca gccggacgca gtatgccatg ccgggtgaac
      601 gaacggaggc gttgtgatgc gattttccct gtttacagac acaaaaaaac gggtttcttc
      661 cccggtgtcg cagcatgacg gagaagcggt atctccggat atgaaagaaa ga
//
LOCUS       DAZUCC010000598          710 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZUCC010000598 DAZUCC010000000
VERSION     DAZUCC010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.601"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            263..457
                     /locus_tag="ACHY09_005465"
     CDS             263..457
                     /locus_tag="ACHY09_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS3888249.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
BASE COUNT          229 a          185 c          147 g          149 t
ORIGIN      
        1 aacggaaacg ccttaaaccg gaaaattttc ataaatagcg aaaacccgcg aggtcgccgc
       61 cccgtaacct gtcggatcgc cggaaaggac ccgcaaaaat gataataatt atcatctaca
      121 tgtcacaacg tgcatctacg ccatcaaacc acgtcaaata atcaattatg acgcaggtat
      181 cgtattaatt gatctgcatc aacttaacgt aaaaacaact tcagacaata caaatcagcg
      241 acactgaata cggggcaacc tcatgtcaac gaagaacaga acccgcagaa caacaacccg
      301 caacatccgc tttcctaacc aaatgattga acaaattaac atcgctcttg agcaaaaagg
      361 gtctgggaat ttctcagcct gggtcattga agcctgccgt cggagactaa cgtcagaaaa
      421 gagagcatat acatcaatcc aaagtgatga tggatgaaca tcccggtttc ttccaccatc
      481 gcaccggaaa agcgactatg agggtaaccc tgcgtctgtc agcacagtaa aacccggtgt
      541 gcatcgtttt tgattattcc cgcacactca cgcagaagga attccccgtc gggctacggt
      601 catggttaat gcgggaatac ggcgacgata cagcgcagct aaaagggtaa tggacagata
      661 gagcggttta tttcattcca caggattctg agtgcccccc ctcctccaat
//
LOCUS       DAZUCC010000599          710 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZUCC010000599 DAZUCC010000000
VERSION     DAZUCC010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.602"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          217 a          170 c          133 g          190 t
ORIGIN      
        1 tgttgcccgt aaacatttaa gttcgcaggc taacattatt gccacaagcc ccgtatttcc
       61 ctggtgaatc ctcaaagcga gtccctcacc atccagacaa aacacagtat cgtcgattaa
      121 taatcactaa aagcgttcgt cgtagcatca taccccctga atgaacacat ccctgtttgc
      181 aaggattctg ttgttctgat atcgacgagt agctgcaact ctgtatccgc acttattgca
      241 atcagtgcag tctggccacg atgcgattcg ctgacctgag ccttattctc caggcggact
      301 ttatgcaatg ctcccctgaa atcttccatt attttacatg tacaggcatc atattaaata
      361 tggaaataat aacgaacgga aaagggacac aatgaatatc aaagcaccag tataaaccag
      421 gatgttttga gcaatttctt actctcctga atacacactg taaatcatta gaaactgcac
      481 tagccatgct aagtacactc tgctacgaac tgatgaggaa gtgcggtatc aattacctgg
      541 acagcctgat caacggagag gtatatctat tatctatgta attacagggg gactgatatc
      601 agcgaaaaag caggtcagca tattcactgc ccctggtctg aactgaaaca acgtcagcca
      661 gaaataatcc tataatgcct catataagtg gataactttt catccgtcgt
//
LOCUS       DAZUCC010000600          708 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZUCC010000600 DAZUCC010000000
VERSION     DAZUCC010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.603"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>708
                     /locus_tag="ACHY09_005466"
     rRNA            <1..>708
                     /locus_tag="ACHY09_005466"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          160 a          155 c          230 g          163 t
ORIGIN      
        1 cccgtgaacc tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg
       61 ggaggctttg aagtgtggac gccagtctgc atggagccga ccttgaaata ccacccttta
      121 atgtttgatg ttctaacgtt ggcccctgat cggggttgcg gacagtgtct ggtgggtagt
      181 ttgactgggg cggtctcctc ctaaagagta acggaggagc acgaaggttg gctaatcctg
      241 gtcggacatc aggaggttag tgcaatggca taagccagct tgactgcgag cgtgacggcg
      301 cgagcaggtg cgaaagcagg tcatagtgat ccggtggttc tgaatggaag ggccatcgct
      361 caacggataa aaggtactcc ggggataaca ggctgatacc gcccaagagt tcatatcgac
      421 ggcggtgttt ggcacctcga tgtcggctca tcacatcctg gggctgaagt aggtcccaag
      481 ggtatggctg ttcgccattt aaagtggtac gcgagctggg tttagaacgt cgtgagacag
      541 ttcggtccct atctgccgtg ggcgctggag aactgagggg ggctgctcct agtacgagag
      601 gaccggagtg gacgcatcac tggtgttcgg gttgtcatgc caatggcact gcccggtagc
      661 taaatgcgga agagataagt gctgaaagca tctaagcacg aaacttgc
//
LOCUS       DAZUCC010000601          698 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZUCC010000601 DAZUCC010000000
VERSION     DAZUCC010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.604"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..266)
                     /locus_tag="ACHY09_005467"
     CDS             complement(<1..266)
                     /locus_tag="ACHY09_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFS3888250.1"
                     /translation="MARLLTIVLFFSISANAVTVNQFQRELDLADKTILWALISASTQ
                     EGRKACSLSYFSCKAAEAELGLAYMAANDNKAFLTSLSRIMMYK"
BASE COUNT          192 a          144 c          116 g          246 t
ORIGIN      
        1 attttgtaca tcattattct agataaggat gtgagaaatg ccttgttatc attagcagcc
       61 atatatgcca gtccgagttc agcctctgcg gctttacatg aaaaatagct aagtgaacac
      121 gcttttcgtc cctcttgggt actggctgat attagtgccc ataatattgt cttgtctgcc
      181 aaatccaact ctctctgaaa ttggtttaca gtaactgcat tcgcagaaat agagaaaaaa
      241 agcacaattg ttaataacct tgccatattt ctagtccgtc actgttaatg tgctatctgg
      301 ctctgaaaat attagctcat ttaatttttg catgtcatta gatgaaccag ttatacaacc
      361 ctcggaacag aatcgaggtc ctaaagatgg accaaaagcg tttaaacaac tatgtgttaa
      421 aaatccatca cgattatacg tttctgtatt tgctgacggt actaaacgta taccattagg
      481 ttttctattg gttgctccag gactgttaag caagcctcgt ccggtttgct tcattgagtt
      541 ctccacttta gactattgga ctatctttca acatggcatc gccttagtat accgctggtt
      601 ttccgctcat tgttaatttt ctctttcgtt catttcactt aattttcagt tttttccaaa
      661 agtcaatatt cccttctaat aaaatttttt ctatggaa
//
LOCUS       DAZUCC010000602          687 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZUCC010000602 DAZUCC010000000
VERSION     DAZUCC010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.605"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..625)
                     /locus_tag="ACHY09_005468"
     CDS             complement(<1..625)
                     /locus_tag="ACHY09_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFS3888251.1"
                     /translation="MNIRNNHLPGTETLFSETETANSKQLAVLKTNFPQCFDKNGAFI
                     QEKLLEIIRASNVEISKESYCLNWLGKSYARLLANLPPTTLLSEDKDHNRREENKNSQ
                     NLLIKGDNLEVLKHMVNAYSEKVKMIYIDPPYNTGSDGFAYNDDRKFTPEQLSDLAGI
                     DLDEAKRILEFTTNGSSSHSAWLTFIYPRLYIARELLREDGVIFISID"
BASE COUNT          199 a          136 c          141 g          211 t
ORIGIN      
        1 cgtcaatcga aataaatatc acaccatctt cacgtagaag ctcacgagca atatataaac
       61 gaggataaat gaacgttaac catgcgctat ggctgcttga accattcgtg gtaaattcaa
      121 gtatacgttt agcctcatca agatcgatac ctgccagatc agaaagctgt tctggtgtaa
      181 atttgcgatc atcattatag gcgaacccat cagaacccgt gttatatggc ggatcgatat
      241 aaatcatttt aaccttttca gaataagcat taaccatatg ctttaatacc tcaaggttat
      301 cccctttgat taacagattt tgactgttct tgttctcttc acgtctgtta tggtctttat
      361 cttctgacag caatgtcgtt ggtggtaggt tagccagcag gcgagcatag gatttgccaa
      421 gccagtttaa gcagtatgac tctttagaga tttcaacatt tgatgctctg atgatctcaa
      481 gcagcttttc ctgaatgaaa gctccgtttt tatcgaaaca ctgtggaaag ttagttttca
      541 aaacagcaag ttgtttactg ttcgcagttt cagtttcaga aaaaagagtt tctgttcccg
      601 gtagatgatt gttgcgtata ttcatacagc acctccagaa agcacggagg tagaggtact
      661 tgacggagag agttcaggcg agcatat
//
LOCUS       DAZUCC010000603          687 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZUCC010000603 DAZUCC010000000
VERSION     DAZUCC010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.606"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>687
                     /locus_tag="ACHY09_005469"
     CDS             <1..>687
                     /locus_tag="ACHY09_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RTX toxin hemolysin A"
                     /protein_id="HFS3888252.1"
                     /translation="IISELGDKDDIVYSGAGSSEVFAGEGHDTVSYNKTDVGKLTIDA
                     TGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQYRDFEFRTGGIPYDVID
                     NLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGNDRLYGDDGDDYISGGQG
                     DDQLFGGSGNDKLSGGDGNNYLTGGRGNDELQAHGAYNILSGGTGDDKLYGGGGIDLL
                     DGGEGNDYLN"
BASE COUNT          220 a           81 c          194 g          192 t
ORIGIN      
        1 gaataatttc tgagctcgga gataaagacg atatagttta ttctggagca ggctcatcag
       61 aagtatttgc tggtgaaggt catgataccg tatcttataa taagacggat gttggtaaac
      121 taacaattga tgcaacagga gcatcaaaac ctggtgaata tatagtttca aaaaatatgt
      181 atggtgacgt gaaggtattg caggaagtcg ttaaggaaca ggaggtgtca gtagggaaga
      241 gaacagagaa aatacaatat cgtgattttg aattcagaac cggtggaatt ccttatgatg
      301 tgatagataa tcttcattct gttgaagaac tcattggcgg aaaacatgat gatgaattca
      361 aaggcggtaa gtttaatgat atattccatg gtgcagatgg gaacgattat atcgaaggta
      421 attatggtaa tgatcgacta tacggcgatg atggggatga ttatatatcc ggtggacagg
      481 gagacgacca gttatttggt ggtagtggaa acgataaatt gagtggaggg gatggtaata
      541 attatctgac aggaggacgc ggtaatgatg agcttcaggc acacggagct tataatattc
      601 tgtcaggtgg tactggtgat gataaacttt atggtggtgg tggtattgac cttctggatg
      661 gaggggaagg taatgactat ctgaatg
//
LOCUS       DAZUCC010000604          686 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZUCC010000604 DAZUCC010000000
VERSION     DAZUCC010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.607"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          235 a          124 c          139 g          188 t
ORIGIN      
        1 aaagttacca aatcttagca cctcagtcag gagagtcagg tcatcgataa agaatattcc
       61 agtagagtgg cgctcatgat caaagataac agcaaagttg atgacagcgc tgaccgactg
      121 tcatttacag aaaccctgtt tgtagcacat tccatcttac ctgacccaga caaagacaaa
      181 gacaaaagag aatgattgtt atttactttt caaaaaaaag tgtcatattg tccatataaa
      241 gtcaagaggt attttacgtt atgtcactga tgcaattgtt agtcaggatc tcatggttgt
      301 cgctcatgta taacctgaga cttgaacgca cactaatagc actgacggtc atacatcggg
      361 aaatgtgagg aggcataaga atgaattgtg ataataatca cagaaatgaa gaattcattg
      421 ttacctttga taaaggcaac aagcaagaca attcaagacg aaaacacgat aattttccta
      481 tagaggtaga atcctccgta gagcaggaga cacactgtat cacaaataat aagtcggctt
      541 ccggtatagt aacacatgac tatgatgccg attatatttg tggttgtggt gaaattatgt
      601 gtcctggttg cggtcatgac ctataacaaa catttttgca aactgatacc tgatcaggta
      661 caagcctgtt gattattatg ctgaat
//
LOCUS       DAZUCC010000605          675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZUCC010000605 DAZUCC010000000
VERSION     DAZUCC010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.608"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>675
                     /locus_tag="ACHY09_005470"
     CDS             <1..>675
                     /locus_tag="ACHY09_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFS3888253.1"
                     /translation="FNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRVKQLAR
                     RKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIAKKLL
                     PYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVARVKV
                     ALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVGNSSE
                     RVPWLCG"
BASE COUNT          189 a          126 c          176 g          184 t
ORIGIN      
        1 ttttaatgaa aaaatagatt tctggtatgt gatagccctc gcaggacagt ctaatggtat
       61 ggcctacggt gaaggtattc ccctgccaga tacactcgac aaacccgagt cccgtgtaaa
      121 acaactggct cgtcgaaaaa caatcacacc aggaggaaaa gagtgtaaat tcaatgagat
      181 tattccggca gatcactgtc tgcatgatgt tcaggatatg agtggatatc accatccggc
      241 tgctgatttg cataaagggg aatatggttg tgtcggacag ggattgcata tagctaaaaa
      301 gttactgcca tacatacctg agcaagcagg gatattactg gttccttgtt gcagaggagg
      361 tgccgccttt actgttggag ctgaaggtat gtatgtacct gatacagggg caacagctga
      421 tgcaatgcgg tggggcaccg gaacagcact ctatgaggac ttagtagccc gggttaaggt
      481 agctctggaa tataacagaa agaataaact gctatctgtc tgctggatgc agggggagtt
      541 tgatcttatg tcaccagatt atgaaaagca tcctgacttg ttttatcaga tggtaacttc
      601 tttcaggtct gagttatctg aatactcatc tcagtgtgtt ggtaattcgt cagaaagagt
      661 tccatggttg tgtgg
//
LOCUS       DAZUCC010000606          673 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZUCC010000606 DAZUCC010000000
VERSION     DAZUCC010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.609"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..280)
                     /locus_tag="ACHY09_005471"
     CDS             complement(<1..280)
                     /locus_tag="ACHY09_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888254.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRV"
     gene            complement(363..>673)
                     /locus_tag="ACHY09_005472"
     CDS             complement(363..>673)
                     /locus_tag="ACHY09_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888255.1"
                     /translation="RNNLSYYSRWYTNPAGNLSVRVCMFRRGGVWTPCLYYRQGMYRN
                     EYTGWYQPLPREVIYLNRFTGRISRRRTWHAACRMAEQLTTLRYRFGLQGDGIVAQPC
                     "
BASE COUNT          183 a          188 c          163 g          139 t
ORIGIN      
        1 tcacacgtat atgccacaga aacggccgga aaagtcctga tttcagttgg tcaccaccgc
       61 ttttgtagcc cagatgacgt gcaatacttt cagtatattc aaatgcatca ttctgcattc
      121 cagccgcctt agccagcgag ctgtacgcat cacgaagtat cgtcatacct tccagttgat
      181 actcacacca cgcacggaca gccgacactg gtgcgattac ccgaagaatg gtgcaggcac
      241 gccatacaac aacgtaaagg agtattaaca gactatgcat gtgttcacct cagatacaaa
      301 aaataacggg aacacacctg ccctgcggga agcgtgttcc cgacagggta ataaatgata
      361 aatcagcagg gctgtgcgac gattccatca ccctgcagac caaaccggta acgcagggtg
      421 gtcagttgct ccgccatacg gcaggcagca tgccatgtcc tgcgacggga tatccgtccg
      481 gtaaaccggt tcagataaat gacctcacgc ggtaacggct gataccatcc tgtgtactcg
      541 ttgcgataca ttccctgccg gtaatacagg cagggtgtcc agacaccgcc acgacggaac
      601 atacagaccc gcacgctcag attgccggcc ggattagtgt accagcgact gtaatatgac
      661 aggttattgc ggg
//
LOCUS       DAZUCC010000607          671 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZUCC010000607 DAZUCC010000000
VERSION     DAZUCC010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.610"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHY09_005473"
     CDS             complement(89..547)
                     /gene="tnpA"
                     /locus_tag="ACHY09_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFS3888256.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          157 a          187 c          125 g          202 t
ORIGIN      
        1 ccggaggttt ttcaaatgcg cctataaggc tctcttacca gccgcgccct aacaggcgca
       61 cacgatctga catttgcatc caacttcgtt acttacggcc cgtaaacggg ctgcccggat
      121 agggaatcga taactgctct cccattttat cctcttcaag ctggtgcttt atgtaatcct
      181 gtatcttcgc cgtgttctta cccaccgtat cgacgtagta ccctctgcac cagaactctc
      241 tgttcctgta tttgaatttc aaatcaccaa actgctcgta aagcatcaga ctgcttttcc
      301 ctttcagata tcccataaag ccggatacac tcattttggg cgggatctcc acaagcatat
      361 ggatatgatc tgcacagcat tcagcttcaa gaatccgtac gcttttccac tcacacagct
      421 ttctcaaaat actgcctatt gctctacgct tctctctgta gaacacttgt cttcggtatt
      481 ttggcgcaaa aactatgtga tatttacagt tccatcgggt gtgcgctaag ctcttttcgt
      541 tccccattgg gacccccttt tgatttcttg tttgacactt gcagttgcca gaccgcaagg
      601 tgttttaaca aatcaaaagg ggttttaata actggctcaa agctgaaagc tttccggaac
      661 ccccagccta g
//
LOCUS       DAZUCC010000608          662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZUCC010000608 DAZUCC010000000
VERSION     DAZUCC010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.611"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..189)
                     /locus_tag="ACHY09_005474"
     CDS             complement(<1..189)
                     /locus_tag="ACHY09_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888257.1"
                     /translation="MFGDEFKTTIECPCCGKICSIAIGQTSHNGILAWYESIICHNCG
                     LRSESDDIGFPPEKIRLQL"
     gene            complement(189..>662)
                     /locus_tag="ACHY09_005475"
     CDS             complement(189..>662)
                     /locus_tag="ACHY09_005475"
                     /inference="COORDINATES: protein motif:HMM:NF026977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888258.1"
                     /translation="PLGLLGGEQTYRYVPNPCGYVDPLGLAITSIINKTVEFSRGPQT
                     YVSDLGHVTGSTARARNKAITAILKEDFAYLKLTYIPQYNPFMRTGIAKLWEGTQFGK
                     NSFSSRAALRDVIIHEELHHRWWKRGLNDHHPKGTEMEQKFYETIRRYKRMRGWL"
BASE COUNT          194 a          159 c          106 g          203 t
ORIGIN      
        1 caattgtaga cgaatttttt ctgggggaaa gcctatatca tctgattcac tacgtaatcc
       61 gcaattatgg catataattg attcatacca tgcgagtata ccattgtgac tcgtctgccc
      121 tatagctata gaacatattt ttccacagca aggacactca atagtcgttt taaattcatc
      181 accaaacact ataaccaccc tctcatccgc ttatatcttc gaatcgtctc gtaaaatttc
      241 tgttccatct cagtaccttt gggatgatga tcattcaatc ctcgcttcca ccacctatga
      301 tgtaattctt catgaataat aacatctctt aatgctgcac gagacgaaaa actattttta
      361 ccaaactgag ttccttccca aagtttagca atccctgttc gcataaacgg attgtattga
      421 gggatatatg ttagtttaag atatgcaaaa tcttctttaa gtatggctgt aattgcctta
      481 tttctggctc tggccgtaga acctgtaaca tgaccgagat cagaaacata agtctgtggc
      541 cctctagaaa actcgactgt tttattaatt atactagtta tagctaatcc cagcggatca
      601 acatacccac acggattagg cacataccgg taagtctgct caccccccag caaccccagc
      661 gg
//
LOCUS       DAZUCC010000609          655 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZUCC010000609 DAZUCC010000000
VERSION     DAZUCC010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.612"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          195 a          151 c          129 g          180 t
ORIGIN      
        1 tgaaactttc agaggaaatg agcgcggccc cccctaataa tcttaagctg ggtacaggga
       61 agtatcgacc attacgcagt gtcgatatcg aatcgtacaa aaaacagtct tggcgtggga
      121 tatttacaca taattattag ctcacaatct ccctatacct gtcgtgtttt ctggggattc
      181 ctcagggagt gaccatagat tccgttttcc aggtgccccc ctttttctcc aaaaattgaa
      241 aaataactct gtagatgccg ctaacaatca attcgagttg atattttaaa gcgttctgtg
      301 cagaaagaaa taaatttcaa aaatgaatca ggggagaaaa gtgcgctttt taacctgttc
      361 agtgtccaga acacatgaaa ccgatcgcca ttcagtttca tgcatagtca tgcaacgcac
      421 tgaaaatgat tctgacgcat ttttccggtt tcccccgagg aaaacatccc tttttgcgtc
      481 agaatcgcgc tcagcgcgtt ttagcggcac gtacaattac aggattataa atcgtcacca
      541 gcgactggcg taaaaaagcc cctctctgca ggcgagattt agatgtttta ttaatcacac
      601 cccaaagctg aatatttatc acaagccatt tttgccgaca aatcactaag gcgat
//
LOCUS       DAZUCC010000610          654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZUCC010000610 DAZUCC010000000
VERSION     DAZUCC010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.613"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..637
                     /locus_tag="ACHY09_005476"
     CDS             <1..637
                     /locus_tag="ACHY09_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFS3888259.1"
                     /translation="AYDVFYSFSMNRDRIQSDVLNKAAELISDMGNKIGDYLGESYKS
                     LAREIADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADRDALVNAWRSRDAQDI
                     ANKLGNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVESWVLSGMAASVAMT
                     LLSAIIGSFTLPALALTAINIAGIMGISYLASYIDASVAEKINNKLVHFTH"
BASE COUNT          209 a           95 c          160 g          190 t
ORIGIN      
        1 tgcttatgat gtattttatt ctttcagtat gaaccgtgac cggattcaga gtgatgtgct
       61 aaataaagca gctgaactta tatctgatat gggtaacaag atcggtgatt atcttggtga
      121 atcttataag tcgttagcaa gagaaattgc tgatgatatc aaaaatttcc aggggaaaac
      181 catccgtagc tacgatgatg caatggagtc attaaataaa attttgtcta atcctagttt
      241 taaagttaac agagcagacc gtgatgcact ggtgaacgcc tggcgaagtc gtgatgctca
      301 ggatatagca aataaactgg ggaatatcag taaggctttc aaagctgccg atgtaattat
      361 gaagattgag aaggtcaagg agaaaagcat cgagggatat gaaactggaa actgggggcc
      421 gctgatgttg gaggttgagt cttgggttct cagtggtatg gccgcaagcg ttgccatgac
      481 attgcttagt gctataattg gatcattcac actaccggcg ttagcattaa ctgcaataaa
      541 tattgcaggt attatgggaa ttagttacct tgcatcatat attgatgcaa gtgtggctga
      601 aaaaataaat aataaattgg ttcattttac tcattgatag aaaaagagag gtga
//
LOCUS       DAZUCC010000611          654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZUCC010000611 DAZUCC010000000
VERSION     DAZUCC010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.614"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..607)
                     /locus_tag="ACHY09_005477"
     CDS             complement(<1..607)
                     /locus_tag="ACHY09_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFS3888260.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCLMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAEN"
BASE COUNT          211 a          126 c          150 g          167 t
ORIGIN      
        1 tgttttccgc caaatatcga gttgcagaaa gtcggcaaat aattaacact ttgtctcctt
       61 tagtgaaaat ttttccttct tcaggctcca gtagtaaata atgtatttgg ccaaattgat
      121 cggtgagttt tccttcgcag ggattacctg aagtggcttg atgaccggta attaacgcca
      181 tgctaccaat atattcttct tctgtaatag ctgagctgtg atcgcgtgga atccagggcg
      241 caactatttt accggtataa tgcaccgcaa taatcgtaaa cagtaaactg acaggaacca
      301 caaacaggtt cgagagtggt gattgccaga ccatgaggca ggtatgctgc aacaggatgc
      361 caataagacc aaaaaaacca gccagtaagc agagaacgac gagagctggc aatcttccga
      421 tattgagata atgaagtgcc tggctaatat gacctgtggt tatggaatca taatggtcaa
      481 gatgtgcatc aagagcaccg gagagcatgt gaccgcagat aagcgcaaaa atttctaaaa
      541 ggccgatcaa cagtacaaag gaaatagcga aaagataagg ggtgttatag tcggcgaata
      601 aaatcatatg tcactccgat gtccatttag tgacgcaata tttactttag gata
//
LOCUS       DAZUCC010000612          638 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZUCC010000612 DAZUCC010000000
VERSION     DAZUCC010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.615"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>638
                     /locus_tag="ACHY09_005478"
     CDS             <1..>638
                     /locus_tag="ACHY09_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS3888261.1"
                     /translation="EPYSLIKAILIAQRANEESEIKSSRVKLSWKKKRQDALESGTIM
                     TASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLNDHAVKNFSGK
                     ESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDLFYAVQEIRLA
                     PFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMRRLHR"
BASE COUNT          209 a          101 c          150 g          178 t
ORIGIN      
        1 tgagccatat tcattaataa aagccatact tatagcacaa agggcaaatg aagaaagcga
       61 gataaagtca agtcgggtta aattatcatg gaagaaaaaa cggcaggatg cactggaatc
      121 aggtacgatt atgacggcgt cttgtccgag atggctctcc ttagatgaca aaagaacggc
      181 ttttgttcca gaccccgaca gggtgaaaac tattgagcta atttttaaac tcaggatgga
      241 aaggcgctca ttgaatgcaa tagccaagta tttaaatgat catgctgtaa agaatttctc
      301 aggaaaagaa agtgcatggg gaccttctgt aattgaaaaa ttattagcga ataaagctct
      361 gataggtata tgcgtacctt catatcgtgc aagagggaaa gggataagtg aaatcgctgg
      421 ctattatccc agagtcatat cagatgattt gttttacgct gtacaggaaa ttcggttggc
      481 accttttggt attagcaata gtagcaagaa tcctatgcta ataaatctac ttcgaacagt
      541 tatgaagtgt gaggcttgtg gtaataccat gattgttcat gcggtatctg gaagtttgca
      601 tggctattat gtttgtccga tgagaagatt acatcgat
//
LOCUS       DAZUCC010000613          633 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZUCC010000613 DAZUCC010000000
VERSION     DAZUCC010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.616"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(84..308)
                     /locus_tag="ACHY09_005479"
     CDS             complement(84..308)
                     /locus_tag="ACHY09_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888262.1"
                     /translation="MKKLRVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(305..>633)
                     /locus_tag="ACHY09_005480"
     CDS             complement(305..>633)
                     /locus_tag="ACHY09_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS3888263.1"
                     /translation="RQRDVAALDAKYSRELADARAENETLRDDVAAGRKRLRINATCP
                     GTVREATGTSGVDNATGPRLADTAERDYFNLRERLMTMQKQLEGAQEYIRTQCTKLAF
                     YYPEDT"
BASE COUNT          146 a          174 c          159 g          154 t
ORIGIN      
        1 gcagaggagt ctccctgtcg ggctgcggtc tctgttaata cgggaatacg gcgacaatac
       61 cgcgccatgg ataataaggt cgctcaacac actggctgta atgcagcgga taccatgcgg
      121 catttagcgg cattcatcgt acactcaacg gttagctctt cattcgtggc attcacctga
      181 aaggtccggg agtgtaattg cgtacattta ccactgaacg aaccttcaac aagaacacga
      241 ccacgctgca aaatacggaa cggaattgtt ccctgaaaag gttctacggt tacccgtaat
      301 ttcttcatgt atcctccgga taataaaaag ccagcttagt gcactgagtg cgtatatatt
      361 cctgcgcccc ttccagctgc ttctgcattg tcatcaaccg ttctctgagg ttgaaataat
      421 cccgttcagc ggtgtctgcc agtcgggggc cggttgcatt atccacgccg gaggtgccgg
      481 tggcttcacg cacggtaccg gggcaggtgg cgttgatccg caggcgctta cgaccagcgg
      541 caacatcatc acgcagagtt tcattttcag ctctcgcatc ggctaattcc ctcgagtatt
      601 ttgcatcgag cgcagcaaca tcacgctggc gca
//
LOCUS       DAZUCC010000614          625 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZUCC010000614 DAZUCC010000000
VERSION     DAZUCC010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.617"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            285..>625
                     /locus_tag="ACHY09_005481"
     CDS             285..>625
                     /locus_tag="ACHY09_005481"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFS3888264.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSD"
BASE COUNT          206 a          129 c          108 g          182 t
ORIGIN      
        1 atttgtgcat agaacatatt tttaatatgc aattaattga caaactaaat cacatttaaa
       61 tacaatatat aaaacaaaat aaccatacaa accaaacaca caaacactta acatatgaac
      121 tacataaaga ttatacagtc ttatccccgt acctggcagc cccttgtttt tcagctatca
      181 gctattttta acacttaaaa attagcatct gtctgcgtca gaggttacgt ctctgcgggc
      241 gtcgttgtgc ttgtagtata ataaataaaa tggagtgtca taaagtggat aaggttttag
      301 attcagcgat cctttcttcg gcaaataaaa gaaagggtat ccttgccatt ggcgcgcatc
      361 ctgacgatat agaattaggc tgtggcgcat cgctagctcg ccttgcgcaa aaaggaattt
      421 atattgctac cgtggtgatg actaccggta attctggcgt agatggaata atcgatcgcc
      481 acgaagaatc acgcaacgct ctaaagatat tagggtgcca ccaaactatt catcttaatt
      541 ttgctgacac ccgcgctcat ttacagctca atgatatgat ttctgccctg gaaaatatca
      601 taaaaaatca aattccctct gatat
//
LOCUS       DAZUCC010000615          619 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZUCC010000615 DAZUCC010000000
VERSION     DAZUCC010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.618"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            158..>619
                     /locus_tag="ACHY09_005482"
     CDS             158..>619
                     /locus_tag="ACHY09_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS3888265.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALEL"
BASE COUNT          199 a          116 c          160 g          144 t
ORIGIN      
        1 tacatttacc aaaatgctaa atatttgtta aatatggcta gtatcaaata ttaagatatc
       61 tgatacctgt ctaaaacagc ataaaagcag gtttcaaaaa tcgtgttttg tacattatga
      121 taccatgcag tgtcaaaaac gataagtgag actaaaaatg gcgttattgg ggtatgcacg
      181 ggtatcaacc agtcatcaga agctaacaca tcaaatctcg gagctaaaaa atgccggggt
      241 acgggaagat cgtattttta cggatatgat gactggcgca actgatgaac gggaaggact
      301 acagcggttg ttggcccgtg cagagaaaga cgacgtaatc atctgtacaa aaatggaccg
      361 actggggcgt aacactgctg acatgattcg tgtggttgat agctgttaca aaaaggggat
      421 cgccatccgg ttcctggaga acggtctcag tacagaaggg acaatgggca aaatggttat
      481 ccaaatcctt gctgctgtag ctgaagcgga acgtgagcgt attctggagc ggacaaacga
      541 cggaagaata gcggcacttg ccgcaggcgt aaaatttggc aggaagaagc atccacgaac
      601 gcctacagcg ttggagctt
//
LOCUS       DAZUCC010000616          618 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZUCC010000616 DAZUCC010000000
VERSION     DAZUCC010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.619"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..248
                     /locus_tag="ACHY09_005483"
     CDS             <1..248
                     /locus_tag="ACHY09_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888266.1"
                     /translation="GLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKE
                     GRVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(266..613)
                     /locus_tag="ACHY09_005484"
     CDS             complement(266..613)
                     /locus_tag="ACHY09_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFS3888267.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
BASE COUNT          155 a          172 c          173 g          118 t
ORIGIN      
        1 caggtctgcc cgcagacggc acttctgcca ctgatctggc ggtggagctg aacggcataa
       61 cgtatcaggc ctgccgtggg gattttgtgg tgcgtctcga cggcagcacc tgtctgcagt
      121 tatggaataa agaaggcaga gtggtacgcc gggagggcga tccgctggaa gtggcgcagt
      181 ggctgcaggc ctgtcacgat gcaggaatgg aggtccgtgt gcaggttaat gaaagcatca
      241 ccccgtaaaa acatgcctgc acggtttaca catcatccca tggtggcagc cggttaactt
      301 tcctgcgcca ccagtggaaa gaggccgtga gcagtccgaa caaaaaacca cccgtgatac
      361 ttataataat ggcctcacct gccaccattc ctgacggccc ccagtagata aaccacattg
      421 cacatcccca ggaaatgccc cataagctcc ccatcaacag cgtgacctgc cagaacggca
      481 taaagggtaa tggcggaagc cggataccca gccgccagag aatacgcaga agaggagggg
      541 cataattgct ccgccacatc tttttgctat ccatcagggc aatggcccgg gcttttttct
      601 gctcaaaggt cacaggag
//
LOCUS       DAZUCC010000617          616 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZUCC010000617 DAZUCC010000000
VERSION     DAZUCC010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.620"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>616
                     /locus_tag="ACHY09_005485"
     CDS             <1..>616
                     /locus_tag="ACHY09_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS3888268.1"
                     /translation="PKNDKGIGNIHEYILAYAKNNEISKEFLMRKDGIDDVYQFIEKL
                     KKEKVPLDKAEQQLKKFYSSNGYDRGITLYNGLNEDYRPWGKINMSWPNADSFGPTYE
                     VLHPLTNNPVKIPDRGWRWKEGTFNHIAKRIDGKYADIKKLHDGSVICGGIWFARTEK
                     TQPSSVKFLDEVEEFLLRSIISTKSDGGVEVENLFGGKGYFSYP"
BASE COUNT          217 a           88 c          134 g          177 t
ORIGIN      
        1 tccctaaaaa tgataagggt attggtaata tccatgagta catccttgct tatgcgaaga
       61 ataacgaaat aagtaaagaa tttttaatgc ggaaagatgg tattgatgat gtatatcagt
      121 ttattgaaaa gttaaaaaag gaaaaggtgc cattagataa agcagagcag caactcaaaa
      181 aattctacag ctccaacgga tatgatcgtg gaattactct ttacaacggt ttgaacgagg
      241 attataggcc atggggtaaa attaatatga gttggcctaa tgcagattca tttggaccca
      301 cgtatgaagt attgcaccct ttgactaata accctgtcaa aatcccagac agaggctggc
      361 gttggaaaga aggcactttt aatcatattg caaaaagaat agatggaaag tatgcagata
      421 tcaaaaaact acatgatggt agcgttattt gtggtggaat ttggtttgcc cgtacagaaa
      481 aaacccaacc cagttcggtt aaatttttag atgaagttga agagttttta ctaagatcaa
      541 ttatttcaac taaaagtgat ggtggtgttg aggttgaaaa tctttttggt gggaaaggat
      601 atttctccta cccgaa
//
LOCUS       DAZUCC010000618          615 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZUCC010000618 DAZUCC010000000
VERSION     DAZUCC010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.621"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>615
                     /locus_tag="ACHY09_005486"
     CDS             <1..>615
                     /locus_tag="ACHY09_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3888269.1"
                     /translation="FGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQ
                     TRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVRE
                     KHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVEL
                     RRSLLSGWVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLL"
BASE COUNT          121 a          184 c          191 g          119 t
ORIGIN      
        1 acttcggcag tcgttccgaa aaagtctccc gccgtatcgc acaaatggaa gccgatctga
       61 accggcttca gaaagagagc gatacgctga ctggtagggt gtatgacccg gcagtacagc
      121 gtccgttgcg tcagacccgc acccgtaagc cgttccctga atcactaccc cgtgacgaaa
      181 agcgactgtt gcctgcggcg ccgtgctgcc cgaactgcgg cggttcactg agctatctgg
      241 gcgaggatac cgccgaacag ctggagttga tgcgtagcgc cttccgggtt atccggacgg
      301 tacgggaaaa acatgcctgt actcagtgcg atgccatcgt gcaggcacct gcaccttcgc
      361 ggcccatcga gcggggtatc gccggaccgg ggctgctggc ccgcgtgctg acctcgaagt
      421 atgcagagca caccccgctg tatcgccagt cagaaatata cggccggcaa ggtgtggagc
      481 tgaggcgttc actgctgtcg ggctgggtgg atgcatgctg ccggctgctg tctccgctgg
      541 aagaggcgct tcatggctat gtcatgactg acggcaaact ccatgccgat gataccccgg
      601 tccaggtact gctgc
//
LOCUS       DAZUCC010000619          611 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZUCC010000619 DAZUCC010000000
VERSION     DAZUCC010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.622"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..189
                     /locus_tag="ACHY09_005487"
     CDS             <1..189
                     /locus_tag="ACHY09_005487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888270.1"
                     /translation="PENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLF
                     VEQVKEWRAISIQAHEKN"
     gene            362..526
                     /locus_tag="ACHY09_005488"
     CDS             362..526
                     /locus_tag="ACHY09_005488"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3888271.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
BASE COUNT          169 a          130 c          166 g          146 t
ORIGIN      
        1 cctgaaaatg aaaagattac ctccgttgat gttgaagact ggacaccgga gcgtcgattc
       61 tcagttgttc ttgagacggc tacgctgtca gaaatggagc ttgctgatta ttaccgacgc
      121 atgggattgt ttgttgaaca ggttaaagaa tggcgggcta tctcaattca ggcgcatgaa
      181 aaaaattgac ggaaaatatt cgaacagaca gggcattgcg tgacgcaaaa aataagttac
      241 gagaactgta gaaagagctt aagcgtaaag ataaagctct ggcggaagct gcggccctgc
      301 ttatattgag ggaaaagttc aatgccctct gggacaacag cgaggaagac tgacccctct
      361 cctggagcgg cgtaaaatta ttgcccttgt ccgcgaggcc gtctccaacg gcgcccggaa
      421 aagtcaggct tgtaggtgta tcggtatttc aattcggaca cttcagcgct ggactgatgg
      481 caacgttatt accacggatg taagcgtcaa cggagcaccg tattgacgct tatttattgg
      541 tgagtactac gttccatggc aggagttcat caactcggtt ggaaggccat tccggcagta
      601 cgctcaggat a
//
LOCUS       DAZUCC010000620          608 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZUCC010000620 DAZUCC010000000
VERSION     DAZUCC010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.623"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>608
                     /locus_tag="ACHY09_005489"
     CDS             <1..>608
                     /locus_tag="ACHY09_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFS3888272.1"
                     /translation="LKDDKGGSATINGGKDNKNGNTLTVKGDGDIAVTVNDKGMTIDG
                     TTLKNEIKNDAIKIKGGDGKTLTKSLGDNVEITGKGPIKTQVAGDKLEISAETASLPV
                     NGGNGKITVNNGDGDKLVTAKNIADAINQSGWKIKADKDTGGVIEGNGQGTETLIKPG
                     DMVTLKAGKNLSVKQSGGDFTFSVIDTPSFSSVQLNGKGNNG"
BASE COUNT          217 a           87 c          162 g          142 t
ORIGIN      
        1 gctaaaggat gataagggag gttctgcgac tattaatggt gggaaggata ataagaatgg
       61 taacacttta actgttaaag gtgatggaga tattgcagtt actgttaatg ataaaggaat
      121 gacgattgac ggaacaacgc tgaaaaatga aataaaaaat gatgccatca aaattaaagg
      181 tggagatggt aagacgctga cgaagagtct tggtgacaat gtcgaaatca ctggcaaagg
      241 cccaataaaa acccaggtgg ctggagataa actggagatt tcagcagaga cagcttcgtt
      301 gcctgtaaat ggtggtaacg ggaagattac agtaaacaat ggtgatggcg ataaacttgt
      361 tactgcgaaa aacattgctg atgccataaa ccagtctggc tggaaaatta aagcggataa
      421 agataccggt ggtgttatcg agggaaatgg tcagggaaca gaaacactga taaaacccgg
      481 tgatatggtt acgctgaaag ccggtaaaaa tctcagcgtg aaacaaagtg gtggtgattt
      541 tacattctct gttattgata ctccgtcatt cagcagtgtt cagctgaatg gaaaaggaaa
      601 taacggaa
//
LOCUS       DAZUCC010000621          605 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZUCC010000621 DAZUCC010000000
VERSION     DAZUCC010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.624"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(81..>605)
                     /locus_tag="ACHY09_005490"
     CDS             complement(81..>605)
                     /locus_tag="ACHY09_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HFS3888273.1"
                     /translation="VSIGPGVALIISESLTYGAKKAITTSIYIRFGEIVVMLVTLFSI
                     ALAINLEKHLDTIKTCGGGYLIFLGITRLIKRKGSKGNKANIPFLIPMLNPKAFLFFA
                     AFMPSFIKNNSNFNTDFLLLCIIFIIVSLLTDLIYICICLTIRGRINSSFSKYVNFIS
                     SLFILLTGAYLVFL"
BASE COUNT          219 a           98 c          110 g          178 t
ORIGIN      
        1 aagtaaggga cgcagagggc gacgaccagc gacgtctgga agagggcgtg atatgtaagg
       61 aagcacaccc tgacggcata tcataaaaaa accagatatg cgccagttaa aagtataaat
      121 aaagagctga taaaattaac atattttgaa aatgatgaat ttatacgccc tcttatagtt
      181 aaacatatac atatatatat taagtcagtg agtaatgaca caattataaa aatgatacaa
      241 agtaacaaaa agtcagtatt aaagttactg ttgtttttta tgaatgatgg cataaatgca
      301 gcaaagaaca aaaaggcctt tggattgagc atcggtatta aaaaagggat atttgctttg
      361 tttcctttag atccttttct cttgatgaga cgagtaatac caagaaaaat cagatatcca
      421 ccaccacaag tttttattgt gtcaagatgt ttttctaaat tgattgccag tgctatagaa
      481 aatagtgtga ctagcatgac aacaatttcg ccaaatctta tatatatact tgtcgttatt
      541 gctttttttg caccatatgt cagactttca gatatgatta atgccacccc cggaccaatc
      601 gatac
//
LOCUS       DAZUCC010000622          600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZUCC010000622 DAZUCC010000000
VERSION     DAZUCC010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.625"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(187..339)
                     /locus_tag="ACHY09_005491"
                     /pseudo
     CDS             complement(187..339)
                     /locus_tag="ACHY09_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
BASE COUNT          193 a          135 c          123 g          149 t
ORIGIN      
        1 tcgcagcaaa aaaacctgca tgtaaagaca gaacagataa aattgctgaa atgctgaaaa
       61 taccattctc tgtcgtcaaa gaatttattg atgaacaggc tttattgaac aaatcaaaca
      121 ttcagggaac tggcagttga acaaaacgcc agtccgaaga ctggcgcagt gataaaacat
      181 tctgcatcat accacattct gctcctgaag atgatgcaga cgatgctccc agtttcccgg
      241 gtaatacccg ggcgtcagca gtacgcggcg tcgctcatta cagagcgagt taccaatcac
      301 tacttctccg tccacgccac acagcgacag ttgtatatag gggtttgggg agcaatgaaa
      361 cagtaaacgc cattaagagg ttaataaaaa tagccaatta tttattaatc aataaattat
      421 atctattcat tgaacgctct cagctccccc ttactggggt ccgcctggaa ttcagaaaat
      481 aaaccacgtt aagactgttt tttagccaaa tgactttcag ggatcgcctg aatggcggga
      541 caacagcggt tttggtgtcc gaaatcgtac aattctgtac atctacacaa gggaattatc
//
LOCUS       DAZUCC010000623          594 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZUCC010000623 DAZUCC010000000
VERSION     DAZUCC010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.626"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..436
                     /locus_tag="ACHY09_005492"
     CDS             <1..436
                     /locus_tag="ACHY09_005492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888274.1"
                     /translation="IKNLINQERDAIALWIKDGMRLPDIYRLLKEKHPELTFSQNGFV
                     FSLRRDASDLHENALFNRSTVAVFMQKQHSEMTLMVNSGCLLKNVHEALFSHISYSVF
                     LRYIVKLYPDLHYQAKLNRKGAVCGELHHEENASVYQNDEVC"
BASE COUNT          175 a           96 c          130 g          193 t
ORIGIN      
        1 gataaaaaac ttaatcaacc aggagcgtga cgccattgca ctctggatta aggatggaat
       61 gagactgcca gatatttaca gattactgaa ggaaaaacac ccggaactga cgttttcaca
      121 gaatggtttt gtattttcac tgagacgtga tgcaagtgat ttacatgaaa atgcgctttt
      181 taatcgcagt actgttgctg tatttatgca gaaacaacat tctgagatga ctctcatggt
      241 taattctggt tgtttgctga aaaatgtgca tgaggcgtta ttcagtcata tatcttactc
      301 cgtgtttctt cgatatattg tgaagttata tccagattta cactatcagg caaaattaaa
      361 caggaaaggg gctgtctgtg gtgaattaca tcatgaggag aatgcttctg tttatcagaa
      421 tgacgaggtt tgttaaaggt gttttataaa aatagtgggg tagtgctggc acagagatgt
      481 cttacaggca ttctcccgca aaaaatgaac ccgttgcttg ttatagtgcc ttgcttaata
      541 cattgcctga atccggagtt actttttaat aattctgtta atggttatta gctc
//
LOCUS       DAZUCC010000624          591 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZUCC010000624 DAZUCC010000000
VERSION     DAZUCC010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.627"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>591)
                     /locus_tag="ACHY09_005493"
     CDS             complement(<1..>591)
                     /locus_tag="ACHY09_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFS3888275.1"
                     /translation="KARQIHEEQLGRTVSLRELADLLTHEGLPIHNSTISRMEDALKY
                     LYPWIPDLMESGLGRPQVTALLALRQDAERVWGQFAVAADTDAEFDRVFGESCRKFNS
                     PELWSLEMFRDELIGDLLQALPHPSLDYDRWLLELDPKERNRRHHFGEPEPVVVPPSV
                     RGSSTVQPGAPVSGIRSEVPECQPDTDTEAMPESTVS"
BASE COUNT          126 a          179 c          150 g          136 t
ORIGIN      
        1 ggagactgtg ctttccggca tcgcctctgt atccgtatcc ggttgacact ccggtacttc
       61 agaccggatc ccggataccg gtgccccggg ttgtaccgta ctgctgccac ggactgaagg
      121 aggcaccacc accggttccg gctcaccaaa atgatgacga cggtttcgct ctttcggatc
      181 cagttccagc aaccaccggt catagtccag ggagggatgt ggcagggcct gcaacaaatc
      241 tccgataagc tcatcgcgga acatctccag tgaccacagt tccggcgaat taaacttccg
      301 gcagctttcc ccgaacacgc ggtcaaattc cgcatccgta tcggctgcaa cagcaaactg
      361 cccccataca cgttctgcgt cctgacgaag tgcgaggagt gctgtaacct gtggacgccc
      421 cagtcctgac tccatcaggt cggggatcca ggggtacaga tacttcaggg catcttccat
      481 tctgctgatc gtggagttat gtatcggcaa cccttcatgg gttaacaaat cagccagttc
      541 acgaagagac actgttctgc ccagttgttc ctcatggatt tgtcttgcct t
//
LOCUS       DAZUCC010000625          588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZUCC010000625 DAZUCC010000000
VERSION     DAZUCC010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.628"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          181 a          125 c          107 g          175 t
ORIGIN      
        1 tcctgtgatc gccaaaatgc ctcttcagtg atccccatcc tgcttatttc tgagcaacct
       61 gaccctgctg atgtatctgg tagtatgaca gagtgattca tttttcttgc cgagtgtacg
      121 aaaacgtctt ccttgccacc gactatcaac cgtcactcaa aaaaacctct ctttttttca
      181 agctatcaag aataagttgc attttatctg caccggacac cagccatcca tatatggctt
      241 ttacctgcta caaagatgaa gaagtgcata aatgtgatgt taaaacaatc aaagaaaaat
      301 tacatgtgca ataatttcac tataacatta taatccatgg tgaaaataac actgcacaag
      361 gattgtcatc atcaaactca tcggaactat tttttctgtc gtcattggtg taatactgat
      421 tgtaagtatc ctttcaggag attatttttg ccgtgtgggg catggaactt gtaaagcagc
      481 caaaagcgct aggcatttaa ttgaaagtaa agagaaaaac agaagtagtc gcgtcaaagt
      541 ctttagcacg catattaatt tgagtcatcc agcggcatcc tggctagc
//
LOCUS       DAZUCC010000626          581 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZUCC010000626 DAZUCC010000000
VERSION     DAZUCC010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.629"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>581
                     /locus_tag="ACHY09_005494"
     CDS             <1..>581
                     /locus_tag="ACHY09_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFS3888276.1"
                     /translation="DGTYSDTTGASESSTRWGVDKPLYKDLIGRTKAALAKNPKNVLL
                     AVVWMQGEFDFSGTPANHTAQFGALVDKYRADLADMVGQCVGGSADGVPWICGDTTYF
                     WKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENGANVPTNKPEEDPDIPGIGYYGSK
                     WRTDRSTWTSQDRASHFSSWARRGIISDRLATA"
BASE COUNT          155 a          132 c          174 g          120 t
ORIGIN      
        1 gatggcacat acagtgacac tactggcgcc tcagagagtt ccacccgctg gggggtggac
       61 aagccgctgt ataaggacct cattggtcgt acaaaagccg cgctggcgaa gaatccgaaa
      121 aatgtgctgc ttgccgttgt gtggatgcag ggggagtttg atttcagcgg cacgccagcg
      181 aatcacacgg ctcagtttgg cgcactggtg gataaatacc gtgcggacct tgcagatatg
      241 gtgggacagt gtgttggtgg ttctgctgac ggtgttccct ggatttgtgg agacacaaca
      301 tatttctgga agcagaagaa cgaaacggcg taccagacgg tgtacggtag ttacaaaaac
      361 aaaacggaaa agaatatcca ttttgtgccg ttcatgaccg atgagaacgg ggcaaacgtc
      421 ccgacgaaca aaccggaaga agacccggat attccgggta tcgggtatta cggttcaaaa
      481 tggcgaacgg acagaagcac ctggacatct caggacaggg ccagccattt cagttcatgg
      541 gcacgacgtg gaattatttc agaccgtctg gcaacggcca t
//
LOCUS       DAZUCC010000627          581 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZUCC010000627 DAZUCC010000000
VERSION     DAZUCC010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.630"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>581
                     /locus_tag="ACHY09_005495"
     CDS             <1..>581
                     /locus_tag="ACHY09_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFS3888277.1"
                     /translation="DGTYSDTTGASESSTRWGVGRPLYKDLIGRTKAALAKNPKNVLL
                     AVVWMQGEFDFNGTPANHAARFTEVVDQYRADLADMAGQCVGGSADSVPWICGDTTYF
                     WKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPDIGYYGSK
                     WRTDRSTWTSQDRASHFSSWARRGIISDRLATA"
BASE COUNT          156 a          127 c          176 g          122 t
ORIGIN      
        1 gatggcacat acagtgacac tactggcgcc tcagagagtt ccacccgctg gggggtgggc
       61 aggccgctgt ataaggacct cattggtcgt acaaaagccg cgctggcgaa gaatccgaaa
      121 aatgtgctgc ttgccgtggt atggatgcaa ggggaatttg attttaacgg aacgccagcg
      181 aatcatgcag cccgttttac agaagtggtg gatcaatacc gtgcggacct tgcagatatg
      241 gcggggcagt gtgtgggtgg ctcagctgac agtgttccct ggatttgtgg agacacaaca
      301 tatttctgga agcagaagaa cgaaacggcg taccagacgg tgtatggtag ttacaaaaat
      361 aaaacggaaa agaatatcca tttcgtgccg ttcatgaccg atgagaacgg ggtgaatgtg
      421 ccgacgaaca aaccggaaga agacccggat attccggata tcgggtatta cggttcaaaa
      481 tggcgaacgg acagaagcac ctggacatct caggacaggg ccagtcattt cagttcatgg
      541 gctcgccgtg ggattatttc cgaccgtctg gcaacggcga t
//
LOCUS       DAZUCC010000628          576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZUCC010000628 DAZUCC010000000
VERSION     DAZUCC010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.631"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..537
                     /locus_tag="ACHY09_005496"
     CDS             <1..537
                     /locus_tag="ACHY09_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS3888278.1"
                     /translation="YYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVSGFDPLG
                     LKVSFQGDESTQKTLKEAYKAVAETKFGHKITEELESSEHEYIFRGLRKGINQTCYDD
                     TEYSFYIDIDNDHSSCVYQGKNKACAMKPTLLSVVLAHEMGHAKGMKDDGTDSMANVD
                     KYENPFRKELGLPARMKY"
BASE COUNT          201 a           87 c          128 g          160 t
ORIGIN      
        1 tattacaacc gccaccgcta ttatgacccg ctgcaggggc ggtatatcac tcaggatccg
       61 attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt atctggtttc
      121 gatccattag ggttaaaagt gtcttttcag ggagatgaaa gtacacaaaa aactttaaaa
      181 gaagcatata aagctgtagc agaaacaaag tttggacata aaataacaga agaattagag
      241 tcatcagagc atgaatatat atttagagga ttaagaaaag gcataaacca aacatgctac
      301 gatgatactg aatatagttt ttacattgat attgataacg accatagctc atgtgtatat
      361 caaggaaaaa acaaagcatg tgccatgaaa cccactctgt tgagtgtggt tcttgcgcat
      421 gaaatggggc atgcaaaggg aatgaaagac gatggaactg actcgatggc taatgttgat
      481 aaatatgaaa acccatttcg taaagagttg ggcttgccag caagaatgaa atattaaaca
      541 tttttatatt gcttttgggt atgtttttaa tatatc
//
LOCUS       DAZUCC010000629          576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZUCC010000629 DAZUCC010000000
VERSION     DAZUCC010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.632"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>576
                     /locus_tag="ACHY09_005497"
     CDS             <1..>576
                     /locus_tag="ACHY09_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888279.1"
                     /translation="YMEYLVTCQLDEEQMSSLLYERDDLSAIDFSLLMKTIKAFSFGG
                     DLQTLASKPGSTISSIPSKRRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAAL
                     PFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAG
                     IWSAFEGVFATKNVLAFSRGDSVNINHSPHDG"
BASE COUNT          175 a          115 c          118 g          168 t
ORIGIN      
        1 ttacatggaa tacctggtaa cttgtcagtt ggatgaagaa caaatgtcat cgctgcttta
       61 tgaaagagat gatttaagcg ccattgattt ttcattgctc atgaagacga taaaagcctt
      121 ttcatttggt ggcgatctcc aaacccttgc atcaaaaccg ggttcaacaa tctcaagcat
      181 cccgtcaaaa agacggatat tgattaacat taaccacgat tttcctaaca atggcaatct
      241 tttcaatgac tttctattta accatcaaca agatgaacaa ttagcaatgg catatatggc
      301 cgctctcccg ttcagcaggc ctttagttta ctgggatggc caagtattaa aatcaacgac
      361 tgaaattaaa aattatgatg ggtcgacgcg tgtcggcggt gaggcgtggc ttaataaagg
      421 ttgctctacc tatcagcagc tctacaatga attccacgca ttatatatag ataaagcagg
      481 aatatggagc gcatttgagg gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg
      541 ggattctgtg aacattaatc actctcctca tgatgg
//
LOCUS       DAZUCC010000630          571 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZUCC010000630 DAZUCC010000000
VERSION     DAZUCC010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.633"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /locus_tag="ACHY09_005498"
     CDS             <1..285
                     /locus_tag="ACHY09_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HFS3888280.1"
                     /translation="AKKSIFKKRVAHGVLVSSLTSAVLGMKLPGEGTIYLRQENNFRK
                     PVFIGDTVKATCEVVDINKEKNIVKLSTITTNQNDEVVIEGIAVVMAPKS"
     gene            311..>571
                     /locus_tag="ACHY09_005499"
     CDS             311..>571
                     /locus_tag="ACHY09_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADH-specific enoyl-ACP
                     reductase/trans-2-enoyl-CoA reductase"
                     /protein_id="HFS3888281.1"
                     /translation="MIIKPVIKGVVSKSAHPEGCRHAIMEQIKYCQSHLQQINNGPKR
                     VLILGASSGFGLAARISLTFGGAKCDSIGVSFEKGPSEKSIGT"
BASE COUNT          184 a          100 c          117 g          170 t
ORIGIN      
        1 gcaaagaaga gtattttcaa gaaacgagta gctcatggtg ttcttgtatc atctctaaca
       61 tcagcagtat taggtatgaa actcccaggt gaaggcacaa tatatcttcg tcaggaaaac
      121 aattttcgca aacctgtttt tattggtgac actgttaaag caacttgcga ggtagttgat
      181 attaataaag aaaaaaatat tgtaaagctt tcaacaatca caacaaatca aaatgatgag
      241 gttgtaatcg aaggcattgc agtagtcatg gctccaaaat cttaatttat ttaactatca
      301 aggaccttat atgattataa aacctgtaat taaaggtgtt gtctcgaaaa gtgctcatcc
      361 agagggatgt cgtcatgcga taatggaaca gattaagtat tgccagtccc atcttcagca
      421 aattaataat ggcccgaaac gagttctgat tctcggggca tcttcaggat ttggtcttgc
      481 tgcaaggatt tctctcactt ttggcggcgc aaagtgcgat tctatcggag tttcgtttga
      541 aaaaggtccc tctgagaaat ctataggaac t
//
LOCUS       DAZUCC010000631          568 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZUCC010000631 DAZUCC010000000
VERSION     DAZUCC010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.634"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>568
                     /locus_tag="ACHY09_005500"
     CDS             <1..>568
                     /locus_tag="ACHY09_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFS3888282.1"
                     /translation="RAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEYYFNPRF
                     LSNDLAESVDLSAFTKGHEAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIPCLST
                     DLLVSLGIKKSALLDNKEHSAEKHVSDNSACTPLRDRLADASSEFNVGQQHLSLSVPQ
                     IYVGRMARGYVSPDLWEEGINAGLLNYSF"
BASE COUNT          147 a          125 c          123 g          173 t
ORIGIN      
        1 cgtgccgcat ttaaggcata tcgtatgcat caggtgttac tactgcctcg ctttgcacgg
       61 cttaccattg cccttggctt agcaactgcg gtttttcccg ttgacgcaga gtattatttt
      121 aatccacggt ttttaagcaa tgatcttgct gaatctgtcg atttatccgc ctttactaag
      181 ggacacgagg caccacctgg tacatatcgg gtcgacattt atttgaacga cgaatttatg
      241 actagtcggg atattacttt tattgcagat gataataacg cagatctcat cccatgcctg
      301 agtacagatc ttcttgtcag tcttggcata aaaaaatctg cattattaga caataaggaa
      361 cattcggcag aaaaacatgt gtcggacaac agcgcatgca caccacttcg ggatcgtttg
      421 gctgacgcat catctgagtt taatgttggt cagcaacatc tatcactctc cgttccgcaa
      481 atttatgttg gcagaatggc tcgcggctat gtttctccgg atctgtggga agaaggaata
      541 aatgctgggc tactaaacta tagtttta
//
LOCUS       DAZUCC010000632          562 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZUCC010000632 DAZUCC010000000
VERSION     DAZUCC010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.635"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..390
                     /locus_tag="ACHY09_005501"
     CDS             <1..390
                     /locus_tag="ACHY09_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFS3888283.1"
                     /translation="GDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGR
                     LIDRDNLKSAMDYYLNTLRRYGELKGGDETAHSFRYSYAASAMKYHIMNGYSYKEALA
                     LTSIDLGHGDGRGRYIEQVYCQLLGYE"
BASE COUNT          179 a           80 c          140 g          163 t
ORIGIN      
        1 ggagatgaaa aaataagagt gatttttggt acgaaagggg gacgggaaag aatgacaacg
       61 gttgttgacc gggataacgt tcttgatgct gttaattatg cactcaaata tgccagtgaa
      121 cataatggac gtcttataga cagagataat cttaagtcgg caatggatta ttatcttaat
      181 acattacgtc gttatggaga actgaaaggc ggagatgaga ctgcacacag ttttcggtac
      241 agttacgctg caagtgccat gaaatatcat attatgaacg gttacagtta taaggaggca
      301 cttgcactga catcaattga tcttggtcat ggtgatggca gaggacgata tattgaacag
      361 gtttattgtc agttgttagg ctatgagtaa taactcatgg cgatttcgta cgggtaaata
      421 ttatggattg tactattttg ctgaaaataa tctctgtaaa agagattaca aaaaattcgc
      481 ctttgtggtc acactatctg gcgcgatatg ttgattccgg atatcaggcc gagtttgaat
      541 gtgaaggaac agcagataaa tg
//
LOCUS       DAZUCC010000633          560 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZUCC010000633 DAZUCC010000000
VERSION     DAZUCC010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.636"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..182
                     /locus_tag="ACHY09_005502"
     CDS             <1..182
                     /locus_tag="ACHY09_005502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888284.1"
                     /translation="MELTVDLLKSLNNIRQNATIYVHAYHYTDDEKCVMVPRISLPSL
                     KLTPLQSEIIILTTS"
BASE COUNT          193 a          106 c          105 g          156 t
ORIGIN      
        1 cgatggagtt gactgtagac ctgctgaaaa gccttaacaa catcaggcaa aatgccacca
       61 tatatgttca cgcataccac tatactgatg atgaaaaatg cgttatggtg ccacggatat
      121 ctttaccatc actgaaatta actccgttgc aatcagaaat aattattctg acaacttctt
      181 aatggagtca attatataag gcacgacggg gatgttcagg gatatgaaga aaaaaatgtg
      241 gcaactttca caatacagga ggagggacac catcgtttca aagcagactt atcacaaaat
      301 gggaaaagtg atatagccag caatgtacta aatcactggc tacatcgtat acataaaaca
      361 gatatcattg ttctgtctgt tgttcttaca gaataaatcc tgttacctcc tgaatgttac
      421 aaggttttat atttatccta tgagaatacg cacgtgaaaa ctaataaaaa aggcacaaca
      481 tggcacattt tttaccgtga aaattcaggc gcggaagttt tgcttgaaat accatcattt
      541 cgggaatgtc tttcagtatc
//
LOCUS       DAZUCC010000634          556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZUCC010000634 DAZUCC010000000
VERSION     DAZUCC010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.637"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>556
                     /locus_tag="ACHY09_005503"
     CDS             <1..>556
                     /locus_tag="ACHY09_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFS3888285.1"
                     /translation="SGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQ
                     LRFEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSAT
                     FMQGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEV
                     SDHYWLRLSAAMKMIGSSQVDYKE"
BASE COUNT          136 a          106 c          190 g          124 t
ORIGIN      
        1 acagtggggt gatcagcagt ataggggatc agactgctgc tgccccggta tggtgggtgg
       61 tggttcataa attgtcgaaa ggtgtcaccc aggcggcagt ggcttcgatt ccgtgccggc
      121 ctgatttgcg ccagttgcgt tttgagatcc agcgaacgtt tatagaaaac agggcacttg
      181 ctgatgagct gcaggacttt acgaacgact gttattcact ggcactgtat atgtggaaac
      241 agcgtgatca ggggcagagt aaagataaaa aagtgcttga tgatatctcc tggataggga
      301 gcgccacatt catgcagggg gattacggga cgctgcagtc aaagatgccg cgggctgcgt
      361 tcccgtggaa tacagcacgt gacagcggac gaccggatac ggggcgggga ggctatccga
      421 catgccggga gtggtggagt gacagtagtg tggggctgaa aaaacgggtc atggaggagg
      481 tcagtgatca ttactggtta cgtctgtcag ctgcaatgaa gatgattggc agtagtcagg
      541 tggattataa ggagga
//
LOCUS       DAZUCC010000635          556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZUCC010000635 DAZUCC010000000
VERSION     DAZUCC010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.638"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..93
                     /locus_tag="ACHY09_005504"
     CDS             <1..93
                     /locus_tag="ACHY09_005504"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M10"
                     /protein_id="HFS3888286.1"
                     /translation="QYSQANAEKSISLGASARAIENNSTALGAK"
     gene            182..>556
                     /locus_tag="ACHY09_005505"
     CDS             182..>556
                     /locus_tag="ACHY09_005505"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3888287.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEAPEILSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELK"
BASE COUNT          165 a          141 c          128 g          122 t
ORIGIN      
        1 caatattcac aggcaaatgc agaaaaaagt atttctttag gtgcatcagc aagagctatt
       61 gaaaataact ctaccgcgtt aggtgcaaag taagcgtaca gcctgaaccg tctggtcaga
      121 atctgacgaa ttagacaaag tggtgtccac caaataagta gtgggaacca aagtatcaga
      181 tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc cctaattatc ctcccgaatt
      241 taaacagcag ctcgttgctg cctcctgtga acccgggata tccatctcaa aacttgctct
      301 tgaaaatggc attaacgcca atctgttgtt caaatggcga caacaatggc gcgagggaaa
      361 actgctatta ccttcttcag agagccccca gctacttcct gtgactctcg atgccgctgc
      421 cgaacagcca gaatcgctcg cagaggcccc ggaaatcctc agtatcagct gtgaggtaac
      481 gttccggcac gggacgctcc gcttcaatgg caatgtcagc gaaaagctcc tgactctgct
      541 gatacaggaa ctgaag
//
LOCUS       DAZUCC010000636          556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZUCC010000636 DAZUCC010000000
VERSION     DAZUCC010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.639"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     repeat_region   127..460
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=127..155
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          137 a          128 c          143 g          148 t
ORIGIN      
        1 ttagcttttt aatcaatgga ttaagtgctc tttaacataa tggatgtgtt gtttgtgtga
       61 tactgtaaag ttggtagatt gtgactggct taaaaaatca ttaattaata ataggttatg
      121 tttagagagt tccccgcgcc agcggggata aaccgagatt tcagaggcag ccgccaggcc
      181 gttgatcgag ttccccgcgc cagcggggat aaaccgccat ttcggtagcg ccatcaaaat
      241 ggttacgcga gttccccgcg ccagcgggga taaaccgcgt cgttagcggt ctggtgacgt
      301 taaaggggtg agttccccgc gccagcgggg ataaaccgtt gatcgccgtg gcggtgagat
      361 tagaaacgcg gagttccccg cgccagcggg gataaaccgg aagtggcaaa gctgccgttt
      421 cttttctcta tgagttcccc gcgcctgcgg ggataaacca ccatataacc cgttatctct
      481 ttctcaagtt tttatattag ctgtacttgt aataagcaac atatccacgt aacacctcat
      541 gttcaaaata gttctc
//
LOCUS       DAZUCC010000637          555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZUCC010000637 DAZUCC010000000
VERSION     DAZUCC010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.640"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>555
                     /locus_tag="ACHY09_005506"
     CDS             <1..>555
                     /locus_tag="ACHY09_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3888288.1"
                     /translation="RDSIAVGQGAVSRGQNSIAMGNGAEVTRQAAAGSVAIGSHARAG
                     RANTGTYALNGQAVAGRTGSDTAVVSFGQPGQERQLQSVAPGVLSANSTDAVNGSQLH
                     ATNRQVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAAAMSGIPQAYLPGKSLM
                     GLGVGGYGGESAIAIGVSRISDNGK"
BASE COUNT          116 a          132 c          184 g          123 t
ORIGIN      
        1 cgtgacagta ttgctgtcgg acagggggcc gtttcccggg gacaaaacag cattgccatg
       61 ggtaatggtg ctgaagtcac gcgtcaggct gctgccggct ctgttgcaat tggcagccat
      121 gcccgtgctg gccgcgcgaa taccgggact tatgccctga acggtcaggc tgttgcggga
      181 cgtaccggaa gtgatactgc ggtggtttct tttggtcagc cgggacagga gcgtcaactg
      241 cagtcagtgg caccaggtgt gttgtctgcg aactcaacgg atgccgtcaa tggcagtcag
      301 ttgcatgcaa ctaaccgtca ggttgccgga aatacacagg cgataaacac tctcgggaat
      361 aaattcagcc agctgagcta ccgggtggaa gagcttaacc gggatatccg tggtgttggt
      421 gcatcggctg cggccatgag cgggatacct caggcctatc tgccgggtaa aagtctgatg
      481 gggctgggtg tcggtggata tggtggtgaa agcgccattg caataggtgt gtcccgtatc
      541 tccgataacg gtaag
//
LOCUS       DAZUCC010000638          555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZUCC010000638 DAZUCC010000000
VERSION     DAZUCC010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.641"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>555
                     /locus_tag="ACHY09_005507"
     CDS             <1..>555
                     /locus_tag="ACHY09_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3888289.1"
                     /translation="RDSIAVGQGAVSRGQNSIAMGNGAEVTRQAAAGSVAVGSHARAG
                     RANTGTYALNGQAVAGRTGSDTAVVSFGQPGQERQLQSVAPGVLSANSTDAVNGSQLH
                     ATNRQVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAAAMSGIPQAYLPGKSLM
                     GLGVGGYGGESAIAIGVSRISDNGK"
BASE COUNT          114 a          131 c          186 g          124 t
ORIGIN      
        1 cgtgacagta ttgctgtcgg acagggggcc gtttcccggg gacaaaacag cattgccatg
       61 ggtaatggtg ctgaagtcac gcgtcaggct gctgccggct ctgttgcagt tggcagccat
      121 gcccgtgctg gccgcgcgaa taccgggact tatgccctga acggtcaggc tgttgcggga
      181 cgtaccggaa gtgatactgc ggtggtttct tttggtcagc cgggacagga gcgtcagctg
      241 cagtcagtgg caccgggtgt gttgtctgcg aattcaacgg atgccgtcaa tggcagtcag
      301 ttgcatgcaa ctaaccgtca ggttgccgga aatacacagg cgataaacac tctcgggaat
      361 aaattcagtc agctgagcta ccgggtggaa gagcttaacc gggatatccg tggtgttggc
      421 gcatcggctg cggccatgag cgggatacct caggcctatc ttccgggtaa aagtctgatg
      481 gggctgggtg tcggtggata tggtggtgaa agcgccattg caataggtgt gtcccgaatc
      541 tccgataacg gtaag
//
LOCUS       DAZUCC010000639          554 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZUCC010000639 DAZUCC010000000
VERSION     DAZUCC010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.642"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..522
                     /gene="dolP"
                     /locus_tag="ACHY09_005508"
     CDS             <1..522
                     /gene="dolP"
                     /locus_tag="ACHY09_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFS3888290.1"
                     /translation="CVAAAVVGTAAVGTKAATDPRSVGAQVDDEVLELRVSSALSKDE
                     QIRKEARISEIAYQGRVLLIGQSPGRELSARARQIALGVDGVQEVYNEIRQGTPVSPV
                     IMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLGLVTEQEGRAAADVASRVS
                     GVKRVTTAFSYIR"
BASE COUNT          135 a          124 c          175 g          120 t
ORIGIN      
        1 tgcgtggctg ccgctgtggt gggtacagct gctgtcggga caaaggcagc aaccgaccca
       61 cgcagtgtcg gagcgcaggt ggatgatgaa gtgctggagc tgagggtcag cagtgcactt
      121 tcaaaagatg aacaaatcag aaaagaagca cgcatcagtg agattgcata tcagggacgc
      181 gtgctgctga tcggtcagtc tccgggccgt gaattatctg cccgtgcccg tcagatagca
      241 ctcggtgtcg atggtgttca ggaggtctat aatgaaatcc ggcaggggac acccgtcagt
      301 ccggtcataa tggcaggtga tgcctggctt acgacaaaaa ttcgttctca gttactcaca
      361 agtgcccgtg tcaaatcatc cagtgtcaag gtggtgacag aaaacagcga agtcttttta
      421 ctgggcctgg ttacagagca ggaagggcga gcggcagcag atgttgccag tagggtgagc
      481 ggagtgaagc gggtcacgac ggccttctcc tatatccggt gaaggcagta tcagagttgc
      541 ctttagtgta aatt
//
LOCUS       DAZUCC010000640          546 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZUCC010000640 DAZUCC010000000
VERSION     DAZUCC010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.643"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..402
                     /locus_tag="ACHY09_005509"
                     /pseudo
     CDS             1..402
                     /locus_tag="ACHY09_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          150 a          129 c          155 g          112 t
ORIGIN      
        1 ccgtcggtgg ccccgcagtg ggtgacgcgg acgaatcatg cggtgagcga cctgacgggc
       61 cgtccgctga tgctctttaa cagtgaaggt aaaaccgtgt ggcgaccgtg acaaaccagc
      121 ctgtgggggc tggcactcag tctgcccgca gacaccggct acccggaccc gcgcggggag
      181 ctggacccgg aagccgaccc cggcctgctg tatgcaggac agtggcagga tacggaatcc
      241 gggctgtgct ataaccggtt ccggtattac gagccggaaa cagggatgta cctggtgagt
      301 gacccgatag ggctgagagg cgggataaac aattatgcat atgttcataa tccccttggt
      361 tatattgatc cattaggatt aaccatttgc gatatcgcaa aaacaaggga tcttgcaaca
      421 acaataagtg ataagtttaa acagcttttt caatgcgata aatttgccgc agcgttaaaa
      481 agtaaaatgc aacaagctgg ggtaaatggt gagcatctca tactcgaaac taaaaataaa
      541 caaggt
//
LOCUS       DAZUCC010000641          543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZUCC010000641 DAZUCC010000000
VERSION     DAZUCC010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.644"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..539)
                     /locus_tag="ACHY09_005510"
     CDS             complement(<1..539)
                     /locus_tag="ACHY09_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFS3888291.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGHWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMP"
BASE COUNT          127 a          177 c          125 g          114 t
ORIGIN      
        1 ggcatcttcc tgaaccggaa ccggtatacg ccctccggta cagatatccc cggctgccag
       61 catctgccgt cggcctgttc gccttccagt tccgccagca acggcaggtg ataaaacaga
      121 gccgaccaga acaccaccat gttccacaca ctcccctgcg cggcctgctc ttctgctgtc
      181 gctcccggtg gaaacattct gcctctggcc agccggacag catacgtcgt gaactgcagg
      241 gtcaggtcac caaaaccacc ggtgtaaccc cagtgacctt ctgctgtggc cggcagattc
      301 tgactgacag acaacaactg atgcacagga tcaagacaaa ggcgctgata catatccacc
      361 ggcatggaac tgttttccca taactgtttc agacaccttt tcctgaacgg tgtggatacc
      421 agtacatcag ccggcaatgg tgtaaaatac ccctccggca ccgtttcccc gggaatatcc
      481 gtccggacat cagagcattc tgtcttcaca ggaaataacc ttttcaggcg ttcaagcatg
      541 ccc
//
LOCUS       DAZUCC010000642          530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZUCC010000642 DAZUCC010000000
VERSION     DAZUCC010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.645"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>530)
                     /locus_tag="ACHY09_005511"
     CDS             complement(<1..>530)
                     /locus_tag="ACHY09_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFS3888292.1"
                     /translation="AYAEPYAGGAGVALELLLEEYVRKIYINDADFAVYSFWSSVVSD
                     TDELCRLISRAKIDMDEWSFHRYVISNPSEFTKLEVGFAAFFLNRTNRSGILKAGVIG
                     GKSQNGKWKMDVRFNRADLISRIEKIANYNQRIVVTNLDALDFLDALNFMDNMGKVDK
                     NKILLYLDPPYYIKGQG"
BASE COUNT          183 a          124 c           67 g          156 t
ORIGIN      
        1 ccttgccctt taatatagta aggaggatcc aaatacaaaa gaattttatt tttatccacc
       61 tttcccatat tatccataaa attcaaggca tcaagaaaat ccaaagcatc cagattagtt
      121 actactattc tttgattata atttgctatc ttctcaattc ttgaaattaa atccgccctg
      181 ttaaagcgaa catccatttt ccattttcca ttttgtgatt tcccgcctat aacccccgcc
      241 ttgagaatac ctgaacgatt tgttctattt aagaaaaacg cagcaaagcc aacctcaagc
      301 ttagtaaact cagaaggatt cgaaataaca tagcgatgaa aagaccattc atccatatct
      361 atctttgctc ggctaataag tcggcaaagc tcatcagtat cactaacaac agatgaccaa
      421 aaagaataaa cagcaaaatc tgcatcattt atatagattt tacggacata ctcttccaat
      481 aataattcta aagccacccc tgcaccacca gcgtaaggtt ctgcatatgc
//
LOCUS       DAZUCC010000643          525 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZUCC010000643 DAZUCC010000000
VERSION     DAZUCC010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.646"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            108..229
                     /locus_tag="ACHY09_005512"
                     /pseudo
     CDS             108..229
                     /locus_tag="ACHY09_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558227.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            257..525
                     /locus_tag="ACHY09_005513"
                     /pseudo
     CDS             257..525
                     /locus_tag="ACHY09_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
BASE COUNT          125 a          114 c          151 g          135 t
ORIGIN      
        1 aggtctttga atttgttagc cagatttctg ttctaatttt ggatagcaga atatgcaatg
       61 ttatttttcg aatcctccat aactgatata tgtgctgtta gtggagtaca agaataagct
      121 cgcgttagca gtctgtcagt actgcgacag cgttgtctgg gagggcttta cccggtcaaa
      181 acagcattat tgtgcacccc atgtgacgga tgaactgtgt gctcaggggg cattttactg
      241 taaaaaccgt gctgcggtca gccgtaaccg tcaggtgggt gaacgcaaca gtcgcgccat
      301 tgcttttcag gcacgtaccc tgcaggcgca tgagcgtcgt atgaacagcc agcagcgtca
      361 gattaatgag aatcatgagg aaatgaagcg ggcagcagcc cagtcagctg cgctggccgg
      421 tcttttccag ccatacagtg tgggtaaatt taacgccaca gcggctgtgg gcgggtacag
      481 tgacaggcag gctgttgcgg tgggcatggg atatcgcttt aacga
//
LOCUS       DAZUCC010000644          524 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZUCC010000644 DAZUCC010000000
VERSION     DAZUCC010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.647"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>524)
                     /locus_tag="ACHY09_005514"
     CDS             complement(<1..>524)
                     /locus_tag="ACHY09_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFS3888293.1"
                     /translation="SDDDNFAKLNTMSYREFLDNLSQTIFAKHDTLHKVFCDIKDTIN
                     ITDYLNIQTIRKIKSGFSKYLLNNSFNKFSLGYNVISGSIHPTKFTNADGNHLHEVLS
                     GDLGVLQDNTKAPLDSYLFEEVFYDSELERLNITDGEIHSVAVFTKIPKNSIKIPVAG
                     GYTYSPDFAYVVKT"
BASE COUNT          163 a           86 c           96 g          179 t
ORIGIN      
        1 tgtttttaca acataagcaa agtcaggaga atatgtgtat ccacctgcca ccggaatttt
       61 aattgagttt ttcggtatct ttgtaaaaac agcaacagag tggatctctc cgtctgttat
      121 attgagtctt tcaagctctg aatcataaaa aacttcttca aaaagataag agtccagggg
      181 ggcttttgta ttatcctgta atactccaag atcacctgat aatacctcat gcaggtgatt
      241 accgtctgca ttagtgaatt ttgtaggatg aattgagcct gaaatcacat tatatccaag
      301 gctgaatttg ttaaatgaat tgttcaacaa atacttgcta aacccagatt taatctttct
      361 gattgtctga atgttcaggt agtcggtgat attgatagtg tcttttatat cacaaaaaac
      421 cttatgtaag gtatcgtgtt tagcaaaaat agtttgtgat aagttatcaa gaaattcccg
      481 atagctcatt gtgtttagtt tagcaaagtt atcatcgtca ctga
//
LOCUS       DAZUCC010000645          523 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZUCC010000645 DAZUCC010000000
VERSION     DAZUCC010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.648"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>523
                     /locus_tag="ACHY09_005515"
     CDS             <1..>523
                     /locus_tag="ACHY09_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004089166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS3888294.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILAD"
BASE COUNT          173 a          107 c          110 g          133 t
ORIGIN      
        1 atatgtccga caacaaaaaa accgtgttac ctgggctaac aacgaccaga aagcccattg
       61 ttacaaaaaa actttcagaa atcgttgccg aagagcttga aagaatgatc cgccaggagg
      121 agataaggga aggtgaatac ctgccttctg aacgtgaact tatggcattc tttggcgttg
      181 gaagaccatc tgtcagagag gctttatcat ctctcagaca taaagggcta atccggtata
      241 acaagggaga aaaagccagt gtctgccgtc catcaccaga aaccatcatt aatgggttat
      301 caggcattgt taaagatttt ttatccacag ctgatggcat caaggatttt gaaagactac
      361 gcatcttttt tgaaaccaac cttgtaagat atgcagcaaa aaatgcaacg cctgaggata
      421 ttcacaggct tgagaaggtg ctcagtatcc atcataactc actgagaacc cccgaagtgt
      481 atgctcaata tgatattgaa tttcatagta tacttgctga tat
//
LOCUS       DAZUCC010000646          523 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZUCC010000646 DAZUCC010000000
VERSION     DAZUCC010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.649"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>523)
                     /locus_tag="ACHY09_005516"
     CDS             complement(<1..>523)
                     /locus_tag="ACHY09_005516"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS3888295.1"
                     /translation="ISEALKNKIIKYITGNNLNLIYDDVHIQNKPMLNYANSARLRKF
                     IIIGTLCEGKGQKIAIEAMHNLIREGYLCHLKIIGNNRVPYASYLNKIVADYNLSDYV
                     EFMGFRHDLDQIRLDNDVCLIPSLSEAFGRVTIESMAAGMIVVASDSGASKEIINDGI
                     NGFLFSSGSVSDLT"
BASE COUNT          179 a          104 c           66 g          174 t
ORIGIN      
        1 gtaagatcac ttaccgaacc tgaagaaaac aaaaaaccat ttataccatc attgatgatc
       61 tctttactag ccccagaatc actagcaaca actatcatac ctgcagccat tgattcaatg
      121 gtaactctac caaaagcttc agaaagagag ggaattaagc aaacatcgtt gtcaagcctt
      181 atttgatcaa gatcatgcct aaatcccata aactctacat aatcactcaa attataatca
      241 gcaacaattt tattaagata acttgcatat ggaactctat tatttccaat tatttttaag
      301 tgacataaat atccttcacg aatcagatta tgcatagcct ctatggctat tttttgacct
      361 ttgccctcac acaatgtgcc aataataatg aacttccgca gcctagccga attagcataa
      421 tttagcattg gtttgttttg gatatggaca tcatcatata ttagattaag attatttcct
      481 gtaatgtatt ttattatttt attttttaac gcttctgata taa
//
LOCUS       DAZUCC010000647          522 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZUCC010000647 DAZUCC010000000
VERSION     DAZUCC010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.650"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..499
                     /locus_tag="ACHY09_005517"
     rRNA            <1..499
                     /locus_tag="ACHY09_005517"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          130 a          127 c          153 g          112 t
ORIGIN      
        1 acaggtgctg catggctgtc gtcagctcgt gttgtgaaat gttgggttaa gtcccgcaac
       61 gagcgcaacc cttatccttt gttgccagcg gtccggccgg gaactcaaag gagactgcca
      121 gtgataaact ggaggaaggt ggggatgacg tcaagtcatc atggccctta cgaccagggc
      181 tacacacgtg ctacaatggc gcatacaaag agaagcgacc tcgcgagagc aagcggacct
      241 cataaagtgc gtcgtagtcc ggattggagt ctgcaactcg actccatgaa gtcggaatcg
      301 ctagtaatcg tggatcagaa tgccacggtg aatacgttcc cgggccttgt acacaccgcc
      361 cgtcacacca tgggagtggg ttgcaaaaga agtaggtagc ttaaccttcg ggagggcgct
      421 taccactttg tgattcatga ctggggtgaa gtcgtaacaa ggtaaccgta ggggaacctg
      481 cggttggatc acctccttac cttaaagaag cgtactttgc ag
//
LOCUS       DAZUCC010000648          520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZUCC010000648 DAZUCC010000000
VERSION     DAZUCC010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.651"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            59..>520
                     /locus_tag="ACHY09_005518"
     CDS             59..>520
                     /locus_tag="ACHY09_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFS3888296.1"
                     /translation="MLWTENDAANTSQWNGYPLQIGRFRKDKAMPALISGEKSAALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSVIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASM"
BASE COUNT          199 a          106 c           90 g          125 t
ORIGIN      
        1 cattcatttg tccagcactc catactttca gccgttggat tggtatataa taaaagccat
       61 gctttggact gaaaacgatg cggcaaatac ttctcagtgg aatggttatc cattacaaat
      121 tggtcgtttt cggaaagata aagcgatgcc tgctttaatc tctggagaaa aatccgcagc
      181 ccttgttacc ccaccacaat ggagaaacaa agcattcaat gggttaaaag atccagaaag
      241 aaattactgg gcaaaagaac aaataactgg ctctccagaa gagaatatca aagcagcaat
      301 tacttactta atgatgaaac tttctaatac aaaggaagaa tcaactatcg atcaatatga
      361 ctcaactcta tatagcgtca tcgtccaaaa aggcgatttg gctgacaaca tcagaaaaga
      421 gagaaaaacg accataccta acttaacgaa aaacaaccct ggaaaaaatc tcgataaaat
      481 acacccggga gatattttat attatcaaaa agcatccatg
//
LOCUS       DAZUCC010000649          520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZUCC010000649 DAZUCC010000000
VERSION     DAZUCC010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.652"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>520)
                     /locus_tag="ACHY09_005519"
     CDS             complement(<1..>520)
                     /locus_tag="ACHY09_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFS3888297.1"
                     /translation="QGRYRVKMDFDREETEPGFGYLWLRMAKPCAGETLGWHTPLIDG
                     TEVAIAYSNGDIDLPYIAYALHDAEHPDLVSRDNHTRNILRTPANNKLRMEDRRNEEH
                     IKLATEYGKTQLNSGHLVDSQGQLRGKGTELRTDEWGTLRAGKGLFVAADAQAKAQGE
                     ALDMDAALKEID"
BASE COUNT           80 a          172 c          132 g          136 t
ORIGIN      
        1 ctgtcgattt ccttcagcgc ggcatccatg tccagtgcct cgccctgtgc tttcgcctgc
       61 gcatccgcag caacaaaaag tcccttccct gctctcaggg ttccccactc atcggtacgc
      121 agctctgtgc ctttaccccg cagttgcccc tggctgtcca ccagatgtcc gctgttaagc
      181 tgtgtcttgc cgtattccgt agcgagtttg atgtgctcct cattgcgtct gtcttccatc
      241 cgcagcttgt tgttcgcagg cgttctgagg atgttccggg tgtgattgtc ccggctcacg
      301 aggtcaggat gctcagcgtc gtgcagtgca tacgcgatgt agggcaggtc aatatcgcca
      361 ttgctgtatg caatcgccac ttcggtgcca tcaatgagcg gcgtatgcca gcccagcgtt
      421 tcacctgcac agggttttgc catccgcagc cacaggtagc cgaatccggg ttccgtctcc
      481 tcccggtcaa agtccatctt cacccggtaa cgcccctgcc
//
LOCUS       DAZUCC010000650          520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZUCC010000650 DAZUCC010000000
VERSION     DAZUCC010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.653"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..>520
                     /locus_tag="ACHY09_005520"
     CDS             101..>520
                     /locus_tag="ACHY09_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888298.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAVTKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAISQNGHMWDAMPGSYYKMGSDWDGGEF"
BASE COUNT          151 a           87 c          150 g          132 t
ORIGIN      
        1 gctgaatgaa taaaatcttc atctggtgtt tttgttgcgc gtaaatgcag gtgtaactgt
       61 ggatgttatc cactgaagag ctgctgatga ggatgaagac atgctgacta aaaaaatgtt
      121 actcgtttgt gcttcgtgtg ttgcgctgag tgcctgttct gcacaggaaa ttgcagagct
      181 gaacaggcag ataagtgacg gtgcatttgc tgtgacaaaa gtactgaacg taggtgtaaa
      241 ccaggcagat gatgatctca aatatctgtc tcaggcaccc cgggcaaaaa gagagaaacg
      301 tgatcatgtc atagaagtcc ctgttgatgt ggatacagct gcgggaagac tgaaacggta
      361 ctataagttt tattcaaatg aagaagtcga tgcgattcgt aataacggac atgcgaacgg
      421 gaagtgggcc gcagcagcaa taagtcagaa cgggcatatg tgggatgcca tgcccggtag
      481 ttattataaa atgggcagtg actgggatgg tggcgagttt
//
LOCUS       DAZUCC010000651          519 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZUCC010000651 DAZUCC010000000
VERSION     DAZUCC010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.654"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>519
                     /locus_tag="ACHY09_005521"
     CDS             <1..>519
                     /locus_tag="ACHY09_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS3888299.1"
                     /translation="GFGIKMKLLPQTENPLVEASVASTEKKKKTLSDNQMLRVYLVVQ
                     RFAEAEEQNMVNSRRRNAIYPAWYWLTVLDVLRYTGMRFNQLKHIRVRDVCPDESIID
                     LKLEGSKTHREWGVPLVAPLKVSMEKLLERARQCGAGDDDYLFDVCRFSEPISADRYQ
                     YCPVRAHQTVRSF"
BASE COUNT          134 a          107 c          151 g          127 t
ORIGIN      
        1 ggattcggga tcaagatgaa actgctgcct cagacagaaa acccgttggt ggaagcatca
       61 gttgcgagta ctgaaaaaaa gaaaaagaca ctgagtgaca atcagatgct cagggtttac
      121 ctggttgtgc agaggtttgc agaagcggaa gagcagaaca tggtgaatag tcgtcgacgc
      181 aatgctatat accctgcctg gtactggctg acagttctgg atgttctgcg ttataccgga
      241 atgcgcttta atcaactgaa gcatatccgt gtgagggatg tctgtccgga tgaaagcata
      301 attgatctta agctggaggg aagtaaaact cacagggaat ggggcgttcc tctggtggct
      361 cccctgaagg tatcgatgga aaaactgctg gagcgggccc gtcagtgcgg ggcaggtgat
      421 gatgactatc tttttgacgt ctgtcgtttc agtgagccga tctctgccga cagatatcag
      481 tactgtcccg ttcgggcgca tcagaccgta cgttcattt
//
LOCUS       DAZUCC010000652          515 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZUCC010000652 DAZUCC010000000
VERSION     DAZUCC010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.655"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..276)
                     /locus_tag="ACHY09_005522"
     CDS             complement(<1..276)
                     /locus_tag="ACHY09_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS3888300.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGIHII"
     gene            complement(273..497)
                     /locus_tag="ACHY09_005523"
     CDS             complement(273..497)
                     /locus_tag="ACHY09_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS3888301.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
BASE COUNT          153 a          137 c           98 g          127 t
ORIGIN      
        1 aataatatgt ataccattcc gttttaaaaa aacctgtgca acaaacaagg ctgttcgctt
       61 atttgcatcg ttgaaaatgt gaccacgagc aatagcaact aaatacacgg cagccagatc
      121 cataatatct gtcacacctt cataatgata catgttcaaa acacgatgaa tgatcgcgtc
      181 agcgcgaccg ggttctggca tacccgccac gccaccatca cgcgaaatca agcggtcatg
      241 aaactcaatc atttcctgtg aactaacgaa tttcatcgct tagaaagctc cgtataggcc
      301 gtcccatgac tttgcatgat ctcagcaaac tcagcatcaa gagccgcttt acggtatcgt
      361 tcaaaagcgt ctttatcaac cagtacagcc gcgccacctt tacggcgctg aatctcaaca
      421 ggctgatttt catcaaccgc cagctcgagc aacgaggcca ggttatcgcg agcatggcta
      481 tagttacatg taaacatagt gcttcctctt tgagt
//
LOCUS       DAZUCC010000653          513 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZUCC010000653 DAZUCC010000000
VERSION     DAZUCC010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.656"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..186)
                     /locus_tag="ACHY09_005524"
     CDS             complement(<1..186)
                     /locus_tag="ACHY09_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFS3888302.1"
                     /translation="MKTSDNESTKYAITGQAVLQILRMKINFSLQTLIKQLLVMKSVE
                     ENAFRRDLIDSIIRDFSN"
     gene            complement(229..465)
                     /gene="ycgZ"
                     /locus_tag="ACHY09_005525"
     CDS             complement(229..465)
                     /gene="ycgZ"
                     /locus_tag="ACHY09_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFS3888303.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRIL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          150 a          109 c           90 g          164 t
ORIGIN      
        1 gttgctaaaa tcacgaatta tgctgtcaat taaatctcgt cggaaagcat tttcctcaac
       61 agatttcatt acgagtaatt gcttaataag agtctgcaac gaaaaattta tcttcatgcg
      121 cagtatctgg agcaccgcct ggccagtaat tgcatatttt gtactttcat tatcagatgt
      181 cttcataacg acttcatagc taacctgtta aataatcagg ctgttgcatt attcaaaaag
      241 caacccaatt agtgcattat aatgtttctg ttcctcttcc cctgtcgctt gttccagtcg
      301 gcatagaagt ttggcacaaa gcgactttcg actcagaata cgtccatctt tcaaaatctc
      361 agtcacaatt tccccgagag tttcctgctg agtaggcaag ttagcttttg caaagtaacg
      421 agtgattgct cccgcagaat ctaaagtcac tgaattttga tgcatgctac gcctctgtta
      481 aaaatgttaa tcaatgttaa gtgaggtaac aat
//
LOCUS       DAZUCC010000654          511 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZUCC010000654 DAZUCC010000000
VERSION     DAZUCC010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.657"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>511
                     /locus_tag="ACHY09_005526"
     CDS             <1..>511
                     /locus_tag="ACHY09_005526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888304.1"
                     /translation="GNYVNSIAENDITAGSGDDRIEIGTNLDGKNKIDLGDGDNTIQV
                     GGYITNSHTITGNSGDDIIYVATNIDGSGSFNLGEGNNNFIVGGYIQGKNTIEMGSGD
                     DTVSVSTRIADNVKIQLNAGDDSVYAGGLLNKAIVDLGDGDDVVTLSGISDNGKRNNM
                     EELVSTNAML"
BASE COUNT          164 a           67 c          130 g          150 t
ORIGIN      
        1 tggtaactat gtaaatagta ttgctgaaaa tgatattacg gctggtagtg gtgatgacag
       61 aatagaaatt ggtacaaatc tggatggtaa aaacaaaatt gatcttgggg atggtgacaa
      121 cactattcag gttggaggat acataaccaa ctcacatacc attactggga atagtggtga
      181 tgatattatc tatgtcgcta caaatataga tggttctggc tcatttaatt taggtgaagg
      241 taataacaac tttatagtcg gaggatatat ccaggggaaa aacacaattg agatgggttc
      301 cggtgatgat acggtttctg tttccactcg tattgcagat aatgttaaaa tccaacttaa
      361 tgccggggat gacagtgtgt atgcaggtgg attgttaaac aaggcaattg ttgacctcgg
      421 ggatggggat gatgtcgtca cattgagtgg aatatcagat aatggtaagc gtaataatat
      481 ggaggagtta gtatccacta atgcaatgct t
//
LOCUS       DAZUCC010000655          508 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZUCC010000655 DAZUCC010000000
VERSION     DAZUCC010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.658"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>508
                     /locus_tag="ACHY09_005527"
     CDS             <1..>508
                     /locus_tag="ACHY09_005527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888305.1"
                     /translation="KNIKVVVDDTGKYEINLNGWDIKDADGAEVKIVDSAGNVKSGNL
                     YNIARLYVDMNTNKAITLDSAYNIIGSQKEGTDPAKALIMSDDNDWVYIGGGISPHIG
                     DQNPGSDNNIDMAGGDDILSSVGAVLDGANINMGDGDDKLYTQDGFASSGTRNIIMGD
                     GNDVISVDN"
BASE COUNT          162 a           71 c          125 g          150 t
ORIGIN      
        1 aaaaatataa aagtagttgt tgatgacaca ggaaagtatg aaataaatct aaatggctgg
       61 gacatcaagg atgcagatgg cgctgaagta aaaattgttg attctgctgg taacgttaaa
      121 tcaggaaact tatataatat cgcgcgtttg tatgtggata tgaatacaaa caaagctata
      181 acgctggatt cggcttataa tataattggc tctcagaaag aaggaaccga tcctgctaaa
      241 gctcttatca tgtcagatga taatgactgg gtgtatattg gtggagggat atctccccat
      301 ataggggatc agaatccagg ctctgacaac aacattgata tggctggcgg tgatgatatt
      361 ctgtcatctg ttggtgcagt tttggatgga gccaatatta atatgggtga tggagatgat
      421 aaactatata cgcaggatgg tttcgcttct tcaggtaccc gaaatattat tatgggagac
      481 gggaatgacg ttatttctgt tgataatt
//
LOCUS       DAZUCC010000656          508 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZUCC010000656 DAZUCC010000000
VERSION     DAZUCC010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.659"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>508)
                     /locus_tag="ACHY09_005528"
     CDS             complement(<1..>508)
                     /locus_tag="ACHY09_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFS3888306.1"
                     /translation="VNAETIRHQYNTHTQDFGVTEWLLAAKSIGLKAKYVEKHFSRLS
                     IISLPALIWRDDGKHYILSRVTKDSSRYLVYDPEQHQSLTFSRDEFEKLYQGKVILVT
                     SRATVVGELAKFDFSWFIPSVVKYRRILLEVLTVSAFIQFLALITPLFFQVVMDKVLV
                     HRGFSTLNV"
BASE COUNT          175 a          106 c           85 g          142 t
ORIGIN      
        1 acatttaacg ttgaaaaccc ccggtgaact aaaaccttat ccattacaac ctgaaaaaaa
       61 agaggtgtta ttaacgcaag aaactgaata aaagcagaaa cagttaacac ctcaagtaaa
      121 atcctcctgt atttcacaac agaggggata aaccaggaaa aatcaaattt agctaactct
      181 ccgactactg ttgctcttga cgtaaccaga atgacttttc cctgatacag tttttcgaac
      241 tcatcccgac taaaagttag tgactgatgc tgttctggat cataaacaag atagcgtgat
      301 gaatctttag taacacgaga caatatataa tgcttaccgt catcccgcca tatcaacgca
      361 ggtaaagaaa ttattgacaa tctggaaaaa tgtttttcta catatttggc ttttaagcca
      421 atggatttcg ctgccagtaa ccattcagtc accccaaaat cttgtgtgtg ggtattatac
      481 tgatgcctta tagtttcagc attgacag
//
LOCUS       DAZUCC010000657          504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZUCC010000657 DAZUCC010000000
VERSION     DAZUCC010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.660"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..88
                     /locus_tag="ACHY09_005529"
     CDS             <1..88
                     /locus_tag="ACHY09_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFS3888307.1"
                     /translation="AMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            152..>504
                     /locus_tag="ACHY09_005530"
     CDS             152..>504
                     /locus_tag="ACHY09_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFS3888308.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEDVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKI"
BASE COUNT          116 a          130 c          152 g          106 t
ORIGIN      
        1 tgcaatggtc tggcgtacct ggaattttga ggacggtgcc gggtactgga tgaaccatgt
       61 catcagggat ttcggcattc ttaagtaaga gaggtgccgg acgggtaggc gtccggcaga
      121 catacaacca ccccggtcaa aggagaaata tatgtcagtt acagagtcta aggcaaaaac
      181 agagcgcaaa tccagccgta aacctgcaaa aacgcaggaa actgtcctgt ctgccctgct
      241 ggcgcagacg gaggatgtga gcgttccgct ggcttcactg attaaatcac cgctgaatgt
      301 gcgcacggtg ccgtattctg cggagtccgt cagcgaactg gctgagtcca ttaagggcgt
      361 cggcctgctg cagaatctgg ttgttcatgc cctgccaggt gaccgccacg gtgtcgccgc
      421 aggtggtcgc cgactggcag cactcaacat gctggcagag cgtggcatca ttccggctga
      481 ctggcctgtc cgcgtgaaaa ttat
//
LOCUS       DAZUCC010000658          501 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZUCC010000658 DAZUCC010000000
VERSION     DAZUCC010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.661"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>501
                     /locus_tag="ACHY09_005531"
     CDS             <1..>501
                     /locus_tag="ACHY09_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFS3888309.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRL
                     TTIQNDRTRIQTVYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQIQNQMDPVYTPARKIHLYHCDH
                     RGLPLAL"
BASE COUNT          104 a          149 c          173 g           75 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtatggcg gcgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccggaag tgacctggta cggctgggac
      121 ggcgaccggc tgaccacgat acagaacgac agaacccgca tccagacggt ttatcagccg
      181 gggagcttca cgccactcat cagggtcgaa accgccaccg gagagctggc gaaaacgcag
      241 cgccgcagcc tggcggatgc ccttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatacaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct t
//
LOCUS       DAZUCC010000659          498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZUCC010000659 DAZUCC010000000
VERSION     DAZUCC010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.662"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>498
                     /locus_tag="ACHY09_005532"
     CDS             <1..>498
                     /locus_tag="ACHY09_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFS3888310.1"
                     /translation="SREKIEKKIARAETRITELQNRLGEAQLQLTSMAGETAPKTSDS
                     PVRKAFPATLPRDRQVISPAETECPVCSGKLKPLGESISEQLDIINTAFRVIETVRPK
                     LACSRCDCIVQAPQPPKPIERSYASPALLARIIMAKFAEHLPLYRQSEIYARQGVELH
                     RNTMG"
BASE COUNT          132 a          153 c          133 g           80 t
ORIGIN      
        1 aaagccgcga gaagatagaa aagaagatcg cacgggcaga aacgcgtata accgagctcc
       61 agaacaggct tggtgaggcg cagttgcaac tcacctcaat ggccggagag acagcgccga
      121 aaacatcaga ctctcccgtc cgcaaagcat ttccggcaac acttccccgt gacaggcagg
      181 ttatctcccc ggcagaaacc gaatgccccg tctgcagcgg caaactgaaa ccgctgggag
      241 aaagcatctc tgaacaactg gatatcatca acaccgcgtt cagggtaatc gaaacggttc
      301 gcccaaaact ggcctgcagc cggtgcgact gtatagttca ggctccgcag ccaccaaaac
      361 ccatcgagcg cagttacgcc agtccggctc tgctggcccg cataatcatg gctaagttcg
      421 ccgagcatct gccgctgtac cgtcagtcgg aaatctatgc ccgccagggc gtggagctgc
      481 accgcaatac gatggggc
//
LOCUS       DAZUCC010000660          495 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZUCC010000660 DAZUCC010000000
VERSION     DAZUCC010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.663"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          125 a          105 c          128 g          137 t
ORIGIN      
        1 tctttaaaaa atagatctat agcgtatcgt tccggcgaac tcatcctgat gcgttttcgg
       61 ggggccatcg gtgaaacata cgaatgatcg ccctctgacg atggatgatt tattttgttg
      121 cacagaagcg atcgctgggc aggtaccgga ggttggctct gaagctctaa ttgcgtcagg
      181 ttgcgaagtg ttgacacggg tattggtgag aactgccccg ggaacgactc tgggctgatt
      241 ctggttctga ttcgctgtca gcccgtgtgg ttactggcct ggctgatttc ctgtcgtcag
      301 gcgtttaact cccgactgca ggtatcactt accaagagaa ggtaaatcct tttagacgta
      361 cagtgaaagt tgtctggtca taatctgaag catctgacaa agttgtgtcc accaaataat
      421 aagtagtggg aaccaaagtg tcatatatac agaaaaatat aactcaccga tttccttcac
      481 gttaatacgc agggg
//
LOCUS       DAZUCC010000661          494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZUCC010000661 DAZUCC010000000
VERSION     DAZUCC010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.664"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>494
                     /locus_tag="ACHY09_005533"
     CDS             <1..>494
                     /locus_tag="ACHY09_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS3888311.1"
                     /translation="KGQKVYWVGGIEGYRTGELEDLYWFQVDMPERMHSDRLRRDYRN
                     FEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLELLRQYTVTNEQDADITVSTAHRSK
                     GLEWPVVMLDEDFQDITDPLMTEAERHDETCLLYVAVTRALRTLVLNDLMHQLAEEQQ
                     KTEG"
BASE COUNT          136 a           99 c          151 g          108 t
ORIGIN      
        1 gaaagggcaa aaggtttact gggtgggggg aatcgaaggt tacaggaccg gggaacttga
       61 ggatctctac tggttccagg ttgatatgcc ggaacggatg cattcagaca ggctgcgccg
      121 ggactacagg aattttgaag aatataaata tattgcaaaa tccacgaagg atgtggagat
      181 gaatcagtcc cttcggcttc tggagttgtg ttttccgtta ccgaaaaaac tggaacttct
      241 ccggcagtat acggtaacga atgagcagga tgcagatatc acggtatcaa cggcacaccg
      301 gagcaagggg ctggagtggc cggttgtaat gctggatgag gatttccagg atatcacgga
      361 tccgctgatg acggaggccg agcgtcatga tgaaacctgt ctgttatatg tggctgtcac
      421 ccgggccctc aggacgctgg tactgaatga cctgatgcac caactggcag aagaacagca
      481 gaaaactgaa ggtt
//
LOCUS       DAZUCC010000662          493 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZUCC010000662 DAZUCC010000000
VERSION     DAZUCC010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.665"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            209..>493
                     /locus_tag="ACHY09_005534"
     CDS             209..>493
                     /locus_tag="ACHY09_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888312.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIR"
BASE COUNT          153 a          117 c          102 g          121 t
ORIGIN      
        1 tttccgtaat ctgatgcatc aggggtatgc ccaccacaaa taactctgta cagcaacaga
       61 aaaatttccc tggtcatatg atgaattaat catcgccatc acaccggatg cttctcatcc
      121 tgtcctcaga aaggcaggtg gtctcctgtc tgccgcgctt atccaggata ccgggacctg
      181 aaaggtcata atctggcagg aaaaaataat gtccgaagta aaaaaacacg tttcttattc
      241 attaacagtt gcagctatgc tgtcagccgc catcgtctgt cagacagcac atgctgatgt
      301 caacacaccg aacatcaccg gagaaaaaaa caatacaaca acacgattct ccggaaatat
      361 ttacggcagt gaaaatactg tcacatcaga gtcaaacagt ctggttgtgg gtaacagtaa
      421 ttccgtggag ggagggtata acaacattgt tattggtaat tcctccacgg tcaaagcttc
      481 cggtttgatc aga
//
LOCUS       DAZUCC010000663          488 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZUCC010000663 DAZUCC010000000
VERSION     DAZUCC010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.666"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..352
                     /locus_tag="ACHY09_005535"
     CDS             <1..352
                     /locus_tag="ACHY09_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3888313.1"
                     /translation="SLTLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRL
                     NAGIAGVTAISSIPYVAENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDS
                     SHNTAIGAGVAGGW"
BASE COUNT          148 a          125 c          112 g          103 t
ORIGIN      
        1 ttcactgaca ctgaaacagg caacagccta cagcgatatc agtacccgcc gggcactgtc
       61 agaggccaat cgttataccg acaacaaatt cagaaacctg agcaataaaa ttgaacgggc
      121 agaaaaaaga cttaatgcag gcatcgccgg agtgacagca atatcctcca tcccctatgt
      181 ggctgaaaat tctttttcat acgggatcgg cgtgggtaac tatcagaacg ctaatgctgt
      241 ggccgcaggc attcagtata aaacatcacg taataccaat atacgcctca acatctcctg
      301 ggactcatct cataacaccg cgattggtgc aggggttgcc ggcggctggt aaataatctt
      361 gcgtacatac ggcattatgg tattccgtaa tcacattaat tcagtacgca acacagcccc
      421 atccggaaca gaccactgga ctgagcggga taatcaagga aaaagggcgc gcagtgtggt
      481 cagccttc
//
LOCUS       DAZUCC010000664          487 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZUCC010000664 DAZUCC010000000
VERSION     DAZUCC010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.667"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>487
                     /locus_tag="ACHY09_005536"
     CDS             <1..>487
                     /locus_tag="ACHY09_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS3888314.1"
                     /translation="PLLRRRLDDKFLRNAENQAFLVETVTAINTIKSMAVSPQMIATW
                     DKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVISLWMGAHLVISGEISIGQLIAFN
                     MLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNTPVEKKSGRNILPEIQGDIEFKN
                     VR"
BASE COUNT          146 a           85 c          124 g          132 t
ORIGIN      
        1 acccttatta cgtcgacgtc ttgatgataa gtttctcagg aatgcagaaa atcaagcttt
       61 tcttgtcgaa acggtaacag caataaatac aattaaatcc atggcagtat cgccacagat
      121 gattgcgaca tgggataaac aactggccgg gtatgttgct tccagtttca gggtaaatct
      181 ggttgcaatg acagggcagc aggggataca gctgatacag aaaagtgtaa tggtaattag
      241 tctatggatg ggagcacatc ttgtcatatc gggagagata agcatcgggc agttaatcgc
      301 gtttaatatg cttgcaggtc aggtcattgc tcctgttatc agactggctc acctttggca
      361 ggattttcag caggttggta tttctgttga gcgccttggg gatgtattaa atacaccggt
      421 agagaaaaag tcaggcagaa atatactgcc agaaattcag ggggatatcg aatttaaaaa
      481 tgtcaga
//
LOCUS       DAZUCC010000665          487 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZUCC010000665 DAZUCC010000000
VERSION     DAZUCC010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.668"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..182
                     /locus_tag="ACHY09_005537"
                     /pseudo
     CDS             1..182
                     /locus_tag="ACHY09_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293042.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            218..>487
                     /locus_tag="ACHY09_005538"
     CDS             218..>487
                     /locus_tag="ACHY09_005538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS3888315.1"
                     /translation="MDGIRIEWKTLGEVAVIGTGNHDTQDAIEHGKYIFYARGREPLK
                     LNVFDFDETAIITAGDGAGVGKVFHYAKGKYALHQRAYRIVPNAFM"
BASE COUNT          149 a           90 c          121 g          127 t
ORIGIN      
        1 ctgtaccaat cccctgcccg gataatccgg aaaaatccct tgccatccag tctgaaatcg
       61 tgcgaattct ggacaaattc agcgccctga cagcagagct gacagcagag ctgacagcag
      121 agcttaacat gcgtaaaaaa caatacaact actatcgaga taagttgttg aatttcaaag
      181 atattgcggg gggggtaatt acatccttcc ggacgccatg gatggtattc ggattgagtg
      241 gaagactttg ggggaagtgg ctgttatagg aacaggtaac catgatacgc aagatgctat
      301 agaacatggc aaatatattt tttatgcacg tggacgtgaa ccattaaagt taaatgtatt
      361 tgattttgat gaaacagcaa tcattacggc tggagatggt gccggggtgg ggaaggtatt
      421 tcattacgct aaaggtaaat atgctttgca tcagagagca tatcgtattg tcccaaatgc
      481 atttatg
//
LOCUS       DAZUCC010000666          486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZUCC010000666 DAZUCC010000000
VERSION     DAZUCC010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.669"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>486
                     /locus_tag="ACHY09_005539"
     CDS             <1..>486
                     /locus_tag="ACHY09_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888316.1"
                     /translation="SLLMLSGCATEALKNEQAETVITAGNKAVAANASFIKQYYASRE
                     NFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFEKEKLLASME
                     VINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVSHSKDLAQSAAAVKD
                     LV"
BASE COUNT          158 a           96 c          107 g          125 t
ORIGIN      
        1 tcgctgttaa tgctttcagg ttgtgcaact gaagcattga aaaacgaaca agctgaaact
       61 gttattactg cgggtaacaa agcagtagcg gcgaatgcaa gtttcattaa acaatactat
      121 gcttctcgtg aaaacttcct tgttaatttc tacgcgacta atccggattg tgccctgcga
      181 cctgattata ccattgatat aggcggtgat gggaatggcg gaaaattatg ccagcgttca
      241 gggccaaata caaaacaagt acaattagtc cctttcgaga aagaaaaatt actggcctca
      301 atggaagtca ttaatagcat gagcgcttat gtggaacttc tgacaaagga tatggcaact
      361 acggaaagtc agcaagtggc attacttaaa caggctctgg atcacgcaaa caacgcacaa
      421 acactacttg gtgtttcaca tagcaaagat ttagctcaat ctgcagcagc ggttaaagat
      481 ttggta
//
LOCUS       DAZUCC010000667          485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZUCC010000667 DAZUCC010000000
VERSION     DAZUCC010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.670"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>485)
                     /locus_tag="ACHY09_005540"
     CDS             complement(<1..>485)
                     /locus_tag="ACHY09_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFS3888317.1"
                     /translation="VAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWVLGWC
                     ERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAGGEFSGEITGVTDGAGRHFRL
                     VLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPDN
                     LP"
BASE COUNT           81 a          176 c          148 g           80 t
ORIGIN      
        1 ggcaggttgt ccggatattc cgggtcgtgc gtcagccaca cggcagacag acggatgccg
       61 ttgtcccggc catattcggt gtaacccggc agggtatcag gaaaagcgga cggttccgtc
      121 ccgccggaaa tggcctgctg ccgggcctct tctgcccgct gcgcctgcgt ggtcagtacc
      181 agccggaagt gacgcccggc accatccgtc acgccggtga tttcgccgct gaattcgccg
      241 ccagcctcac ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc
      301 cggtacggcg gcagcggcgc aggcagcacc tcatccgcct ccggcacccg ctcacaccag
      361 ccgagtaccc accacggccc ctgcggactg ttcgtcgcca gataacgatg cggactcagg
      421 cgaagctctt ccggcagcgc ctgccagagt gcggccagcc gatgaccttc atccagtttc
      481 gccac
//
LOCUS       DAZUCC010000668          483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZUCC010000668 DAZUCC010000000
VERSION     DAZUCC010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.671"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..100
                     /locus_tag="ACHY09_005541"
     tRNA            24..100
                     /locus_tag="ACHY09_005541"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHY09_005542"
     tRNA            143..218
                     /locus_tag="ACHY09_005542"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
     gene            402..>483
                     /locus_tag="ACHY09_005543"
     rRNA            402..>483
                     /locus_tag="ACHY09_005543"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          136 a          100 c          127 g          120 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
      361 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      421 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      481 ggt
//
LOCUS       DAZUCC010000669          483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZUCC010000669 DAZUCC010000000
VERSION     DAZUCC010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.672"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            65..>483
                     /locus_tag="ACHY09_005544"
     CDS             65..>483
                     /locus_tag="ACHY09_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888318.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGFLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLV"
BASE COUNT          139 a           95 c          135 g          114 t
ORIGIN      
        1 tcgtgtgctc cgggaaatca ggagaatgag cactactgtc attcacatca aaggggaatg
       61 agatatggct attgcagaga gaacgggtat ctcctcgcgc ggcgatactt acgaaatctg
      121 gttgggagca aactgttatc actaccgctc tgatggttat gcagtgcttc tgaaagaagg
      181 caggaatcgt gcttatccat gtcgggtgac gagtgaaacg attaaatctg cgctggcagc
      241 aattgttgaa ttgcaacaga aattaactgc ggcagagaag aaaaaggagt gtgagaacca
      301 cgggtttctt ttgccagctg agatgttaat tcgctgtctg gaagatcaaa cagggggacg
      361 gtggatccgt gcacatgaac cagtaatgct gatgaagcga ttaatcagta acctgaaaaa
      421 agccagacca gaatgtaccg ttcctgatga agcgctggca tatctccggc gtaactgtct
      481 ggt
//
LOCUS       DAZUCC010000670          480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZUCC010000670 DAZUCC010000000
VERSION     DAZUCC010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.673"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(295..>480)
                     /locus_tag="ACHY09_005545"
     CDS             complement(295..>480)
                     /locus_tag="ACHY09_005545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888319.1"
                     /translation="PPYSKNTQAFRTLYQPHLDCFPFPNTAARGVIKVRNFQQYGVLL
                     FTAVMHQPVLAIVVITV"
BASE COUNT          142 a           99 c          110 g          129 t
ORIGIN      
        1 ctctacatat cgacgaaaaa accacttgat ttgacaggaa cacattcaaa aaaatttctt
       61 tattcatttc aggagcgatt ttcatctgtt acttgaagta tctgtgagat taagtgactc
      121 cccctaacgt cgtactaaat agtaattact gtccataccg ggagcagtat tcggcgctcc
      181 ccacatgcag cagaataacc gcatcagcga agatgtattg tatttttact atcacaatgt
      241 gtacggacgg tttatggaaa agaatgaaaa ttagatacgc gatggggcgg caggttacac
      301 ggttatcact acgatagcca atactggctg gtgcattacc gccgtgaaca ataggacgcc
      361 gtactgctgg aaatttctta cctttatgac tccccgcgcc gccgtattgg gaaacgggaa
      421 gcaatcaaga tgaggctgat ataatgtgcg gaatgcctga gtatttttag aatagggggg
//
LOCUS       DAZUCC010000671          475 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZUCC010000671 DAZUCC010000000
VERSION     DAZUCC010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.674"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..460
                     /locus_tag="ACHY09_005546"
     CDS             98..460
                     /locus_tag="ACHY09_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888320.1"
                     /translation="MKKYILVSLMGLSLNAFAGGSGWNPDRVDPSRCVMATGSGPTSA
                     GYMVYRTTDSVCMQGINEGKVKGVHIYDGHHEFNDGTVDTFSGNVTPISPLTLKTDVQ
                     KVNKVGIKRWWYKASWIK"
BASE COUNT          151 a           74 c          119 g          131 t
ORIGIN      
        1 aagagcttgc ataagtaagt gactgaggga gcgtaagtgc agtacatgca acttgttgca
       61 tgacaaatat attatctatt aacaataaga ggttataatg aagaaatata ttttagtatc
      121 tctgatggga ttaagtttga acgcatttgc gggtggcagc ggctggaacc cagacagggt
      181 agacccgagc cgttgtgtta tggcgacggg gtcaggtcct acctctgcgg gttatatggt
      241 ataccgaacg actgactcag tgtgtatgca gggtataaat gaaggaaaag tgaaaggtgt
      301 ccatatttat gatgggcatc atgagttcaa tgatggtact gttgacacgt ttagcggtaa
      361 tgttacccca atatcacctt tgacattaaa aactgatgtt caaaaagtta ataaagttgg
      421 catcaaacgc tggtggtata aagctagctg gattaaataa aaaatacctt gccaa
//
LOCUS       DAZUCC010000672          473 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZUCC010000672 DAZUCC010000000
VERSION     DAZUCC010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.675"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>473
                     /locus_tag="ACHY09_005547"
     CDS             <1..>473
                     /locus_tag="ACHY09_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888321.1"
                     /translation="GNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGS
                     DTLKRRITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITET
                     RARTEGDAAALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQAR"
BASE COUNT          150 a          124 c          116 g           83 t
ORIGIN      
        1 tcggtaatgc caccagaatt aacggtgatt catctgttgc cataggtctg acatcgtccg
       61 taaccgggaa cgactccgtg gcactgggca gtcattccag tgctaacgga aacaatatcg
      121 tatccgtcgg ctctgacaca ctgaaacgcc gcatcacaaa tctggcacag gggaaagatg
      181 accatgatgc ggtaaatctg atgcagatga ccagtgctgt taaccgggaa gctggagaaa
      241 gaataaaatc aacaaacaac ctgcagaatc agcttaatac cctctctggt caggtcataa
      301 ctgaaaccag ggcccgaaca gaaggtgatg cagcagcgct ggctgcagcc agagcccaca
      361 gcgatgaaaa cgacaaaaga acactggtca gtgcgaatac ctatgctgac cagaaagaaa
      421 ccgccatcaa taaccgtact gacggattac tgacgaccga acgccaggca cgc
//
LOCUS       DAZUCC010000673          473 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZUCC010000673 DAZUCC010000000
VERSION     DAZUCC010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.676"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHY09_005548"
     CDS             complement(165..>473)
                     /gene="hcp"
                     /locus_tag="ACHY09_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFS3888322.1"
                     /translation="TKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYFNTK
                     LENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNERTTA
                     "
BASE COUNT          112 a          128 c          108 g          125 t
ORIGIN      
        1 ttcactcact gtcagaagac cttcagacac gctaccgccc ttcgccataa cccgggcgca
       61 tcaggtctgt gttcaggatt gagaattctt tcttcagcac ccgccaacag atactgaaga
      121 aaaaagaaaa atgacggaca ggataccctg cccgtccggg agaattacgc agtggtacgt
      181 tcattccacg cgtcagaatg aatgatgtta ccgtccttat aagtccaggt gattttctcg
      241 tagcgcagct ccacctgctc aaggtggtta tgcttctcgt aggcgggatt cttgatgtcg
      301 tgcatgaccg ggttaacctt caccaccttc acgttctcaa gcttcgtatt gaaatactcc
      361 acttcctgac cggcatcatt gattctgtac cacttaaatt cagcagactt cagggtctgc
      421 cccgtggtca ccgccttgta aaggtagggg ctggaggaat caatttcctt ggt
//
LOCUS       DAZUCC010000674          471 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZUCC010000674 DAZUCC010000000
VERSION     DAZUCC010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.677"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..460)
                     /locus_tag="ACHY09_005549"
     CDS             complement(<1..460)
                     /locus_tag="ACHY09_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS3888323.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIE"
BASE COUNT          132 a           99 c           97 g          143 t
ORIGIN      
        1 actctatttg atatgatttc agacgctcga tggctgctat cacttttgca aaagtaccgt
       61 tacctgaatt actgcgtctg taacggtcat gtagctcttg ggggccatcg atcgagatac
      121 caaccagaaa ctcatgttct ttgagaaagg cacaccattc attattcaat aaaatgccat
      181 tcgtttgtaa tgcattaaaa atacgttttt ggcctgcata gcgttgttga tagtgaataa
      241 ctttacggaa aaaatccagg ccagccagag tgggttcacc gccttgccag gtaaaataga
      301 cctgattgcc agacgctgcg atatattgtt tgatgaactc tttcagagtg ctgtcatcca
      361 tccatttttc atgagtaaac tgcgactctt tttcaaggta aaaacagtaa tcacatttga
      421 gattacattg aaaactggag ggcttggctg taacgtgcat cgctatctcg c
//
LOCUS       DAZUCC010000675          469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZUCC010000675 DAZUCC010000000
VERSION     DAZUCC010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.678"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>469)
                     /locus_tag="ACHY09_005550"
     CDS             complement(<1..>469)
                     /locus_tag="ACHY09_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS3888324.1"
                     /translation="YYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPITNIDPLG
                     LETLKCIKPLHSMGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDG
                     LWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPIN"
BASE COUNT          122 a          122 c           84 g          141 t
ORIGIN      
        1 aatttatagg tgtgaatata tcaggcaata cagaataacg cggccttacc tctttaaact
       61 tcccttttaa acagttctcc acacaactat tatcggtttc aacgaggtca caacggccag
      121 cagcttcttt tgtatcatta cttgctttac ctggccccca tagaccatct ccttttgatt
      181 ctccccgttg gtcttggcca ccacaagtat agtccccttt accatctggg acacaaagat
      241 attgatgata gaacggattc ccccatatat ctggaccgct tctttcacca gttccgccca
      301 ttgaatgcag tggcttaatg cattttagtg tttctaatcc taaaggatct atattagtga
      361 tcggattcaa cggatactga taaaaattcc atcccccctt aagcccaatc ggatcctgag
      421 tgatataccg cccctgcagc gggtcataat agcggtggcg gttgtaata
//
LOCUS       DAZUCC010000676          465 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZUCC010000676 DAZUCC010000000
VERSION     DAZUCC010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.679"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..311
                     /locus_tag="ACHY09_005551"
     CDS             <1..311
                     /locus_tag="ACHY09_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888325.1"
                     /translation="NIALEQVVTWDSDNIFTPNIFEHNGRCFMKKNETWMLLNGDATP
                     NIRTMSNKKIECLEKSEVEWASRVCDSITDKERENLKALICSKPAKSVNTGKRPALGY
                     "
BASE COUNT          175 a           86 c          104 g          100 t
ORIGIN      
        1 ataatatagc cctcgagcaa gtcgtaacat gggattctga taatatattc acacctaata
       61 tctttgaaca taatggaaga tgttttatga agaaaaatga aacatggatg ttattaaatg
      121 gagatgcaac accaaacatt agaacaatga gcaataagaa aatagaatgc ctggagaaga
      181 gtgaagtaga atgggctagt agggtttgcg acagtatcac agacaaggaa agggaaaatc
      241 tgaaggcatt aatatgtagc aaaccagcta aatcagttaa tacaggcaaa agaccagcgt
      301 taggatattg aaaccggcac tgcactctat cactattaca atattctgag tgactagagc
      361 aactgctccg ccatccgggc tagcagtatg acgacgaatc cgaactgcac tacaactggc
      421 accggtacta caatccggga cagggactag ggaaagatca cgcaa
//
LOCUS       DAZUCC010000677          460 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZUCC010000677 DAZUCC010000000
VERSION     DAZUCC010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.680"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>460
                     /locus_tag="ACHY09_005552"
     CDS             <1..>460
                     /locus_tag="ACHY09_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS3888326.1"
                     /translation="KMMRDFRFLMPESKEEQEKVVRIIQKIDELKRLNNAQNQTLEQM
                     FQALFKSWFVDFDPVIDNALDAGNPIPETLQSRAELRQNVRNSTDFKPLPAEIRSLFP
                     SEFEETELGWVPKGWESETFDSFCDLIQSGGTPSRKETSFWDGGTIKWLSS"
BASE COUNT          147 a           83 c          107 g          123 t
ORIGIN      
        1 taaaaatgat gcgtgatttc aggtttttaa tgcctgaatc aaaagaggag caggaaaagg
       61 ttgttcgtat aatacaaaaa attgatgaac taaaaagatt aaataatgct caaaatcaaa
      121 ccctggaaca aatgttccaa gccctgttca aatcctggtt tgtagatttt gatccggtaa
      181 ttgataacgc tctggatgca ggaaatccaa tcccggaaac cctgcaatct cgtgccgagt
      241 tgcgtcaaaa tgtacgtaat agtacagatt tcaaaccgct tccggcggaa attcgctcgc
      301 ttttcccaag tgaatttgaa gaaacggagt tgggttgggt gccaaagggt tgggaaagtg
      361 aaactttcga ttcattctgc gacttaattc agagtggggg aacgccttca aggaaggaaa
      421 cttcattctg ggatggcggt acaataaaat ggctttcatc
//
LOCUS       DAZUCC010000678          457 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZUCC010000678 DAZUCC010000000
VERSION     DAZUCC010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.681"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..>457
                     /gene="adiY"
                     /locus_tag="ACHY09_005553"
     CDS             52..>457
                     /gene="adiY"
                     /locus_tag="ACHY09_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFS3888327.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKF"
BASE COUNT          130 a          106 c           89 g          132 t
ORIGIN      
        1 cgctttgctt tagcgagaac tggtctttta ttcgcactca ggagtacatg tatgaggatt
       61 tgcagcgacc aaccttgtat tgttttattg actgaaaaag atgtctggat aagggtgaat
      121 gggaaagaac ctattagcct taaagctaac catatggcgt tattaaattg tgaaaataat
      181 attatcgacg tctcctctct taataacact ttggttgctc atattagtca cgacatcatc
      241 aaagattacc tccggtttct gaataaagat ctctcgcaaa taccagtatg gcaacgtagc
      301 gctacgccca tcctcaccct tccatgcctg acgccagacg tctttcgcgt tgccgcgcaa
      361 catagcatga tgcccgctga aactgagtca gaaaaggaac gaacacgtgc attattattc
      421 actgtgctat cccgttttct cgacagtaaa aaattcc
//
LOCUS       DAZUCC010000679          456 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZUCC010000679 DAZUCC010000000
VERSION     DAZUCC010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.682"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(27..128)
                     /locus_tag="ACHY09_005554"
                     /pseudo
     CDS             complement(27..128)
                     /locus_tag="ACHY09_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(384..>456)
                     /locus_tag="ACHY09_005555"
     CDS             complement(384..>456)
                     /locus_tag="ACHY09_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFS3888328.1"
                     /translation="EQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          132 a           94 c           92 g          138 t
ORIGIN      
        1 aatcatttgg acgttataac ctaaattttt accgtccaat aatcaatatt tctgttgtga
       61 ttgttaacca ctgcgtgcat tggcgggatt gaagggtgaa aaatatgtgc aattgggaag
      121 cctttcattg atgcctgcct tgtcatcatg acaaacaata atagatgctt attttcctcg
      181 cagggttctt tcgcactatc aaaaaatgct catttacccg gcattacagt caacgaagca
      241 acattctatg gcctacgctg atgtaaagca cagtgagaat cccccttgtt gtcttcgtct
      301 gaccttatcg cggcgaccag caattattcg ttttgtgatc ctcaacacaa aatgctattc
      361 gtcgcctatg aggggagagg aatttatgga aaacgattgc attcacaaaa tgacgtcctg
      421 tcagaagaga aaaagttgac gttaatcaat tgttcg
//
LOCUS       DAZUCC010000680          455 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZUCC010000680 DAZUCC010000000
VERSION     DAZUCC010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.683"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..221)
                     /locus_tag="ACHY09_005556"
     CDS             complement(<1..221)
                     /locus_tag="ACHY09_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888329.1"
                     /translation="MIRIIVFLLFFLSGCTSQKEYSNIVRIENRCSSDIDVFIQGYVS
                     GSTLIYQHNIHLPVGDSDVLVYFSTADID"
     gene            complement(214..>455)
                     /locus_tag="ACHY09_005557"
     CDS             complement(214..>455)
                     /locus_tag="ACHY09_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFS3888330.1"
                     /translation="SAHEMGHKVMDKYGSESWTHKGTSTRGQSTIPNTPMPSSGEIDI
                     MKYADSYAPDRYKRLVAADEDVQALIWLSRVSFND"
BASE COUNT          148 a          106 c           65 g          136 t
ORIGIN      
        1 tcatcaatat ctgccgtgga aaaatatact aatacatcac tatcaccaac tggcaagtgt
       61 atattatgct gataaatcag cgtagaaccg gaaacatatc cctgaataaa gacatcgata
      121 tctgaggagc atctattctc aatcctgact atattggaat actctttttg tgaagtacac
      181 ccactcaaaa aaaacaataa aaaaacaatt attctaatca ttaaaactca ccctgctcaa
      241 ccagatcaat gcctgaacat cttcatctgc cgcgaccaat ctcttatatc tatcaggtgc
      301 atagctatct gcatatttca ttatatcaat ctctcctgaa gatggcattg gagtattagg
      361 gatggtactt tgaccccgcg tggatgtccc cttatgtgtc catgattcag aaccatattt
      421 atccataact ttatgtccca tctcatgagc actat
//
LOCUS       DAZUCC010000681          453 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZUCC010000681 DAZUCC010000000
VERSION     DAZUCC010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.684"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>453)
                     /locus_tag="ACHY09_005558"
     CDS             complement(<1..>453)
                     /locus_tag="ACHY09_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3888331.1"
                     /translation="MFPDAVLSLLAPFASTVQGGQRTRRLILLIWLCVLTALGISALN
                     NRDLIRQVSTDLQRWNAVPKGHYRPEAESLAALKQDALLLEQWQRQGVPLRYSLGYYP
                     GQRLWLALQQAIDTRVPPPSPEPEPVPEIVRLDSLALFDAGKWTLKPG"
BASE COUNT          104 a          139 c          126 g           84 t
ORIGIN      
        1 cgcccggttt cagcgtccat ttaccggcat caaacagtgc caggctatcg agtcgtacaa
       61 tttccggcac cggttctggt tcaggggatg gtggtggaac ccgggtatcg atagcctgct
      121 gcagggccag ccacagacgt tgtcccgggt aatagcccag gctgtaacgt aaaggcactc
      181 cctgacgctg ccactgctca agcagaagcg catcctgttt cagtgcggcc agcgattccg
      241 cctcaggacg gtagtgcccc ttcgggacag cattccagcg ctgtaaatcg gtgcttacct
      301 ggcggatcag atcacgattg ttaagcgcag aaatccccag cgcagtcagg acacacagcc
      361 atatcagcaa aatcagacga cgggtacgct ggcctccctg taccgtcgac gcaaaaggtg
      421 ccagcagcga cagaaccgca tccgggaaca tcc
//
LOCUS       DAZUCC010000682          451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZUCC010000682 DAZUCC010000000
VERSION     DAZUCC010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.685"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(219..>451)
                     /locus_tag="ACHY09_005559"
     CDS             complement(219..>451)
                     /locus_tag="ACHY09_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888332.1"
                     /translation="LYRSALAFIQALIALVPSKKILDLDKITDALRRRYVHLEQLEYA
                     ELRNALACALSTCYNRKYLIKVAKGKYLPVDD"
BASE COUNT          128 a           96 c           82 g          145 t
ORIGIN      
        1 tcatatctcc attttgatac aactgaggca ctgatcttta gcttacgggc tatctgacga
       61 tgtgaccagc agtatggtgg taaaagccat atcagtcctt tctctttgag ctcacttggt
      121 attggttgtc ctggcattgt tatctcctta aaaaattgag gcgacaacta tgctgacaca
      181 gggggttact caccgatctc cttcacggta atacgcacct agtcgtcaac aggtaaatat
      241 tttccttttg caactttaat gagatatttt ctattataac aagttgacaa tgcacaagct
      301 aaagcatttc ttaattcggc atattctaat tgctcaagat gaacataccg cctacgcaga
      361 gcatctgtta tcttatccaa gtccagtatt ttctttgatg ggactaatgc aatcaaggcc
      421 tgtataaaag ctaatgcact tctgtaaaga a
//
LOCUS       DAZUCC010000683          450 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZUCC010000683 DAZUCC010000000
VERSION     DAZUCC010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.686"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(116..322)
                     /locus_tag="ACHY09_005560"
     CDS             complement(116..322)
                     /locus_tag="ACHY09_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFS3888333.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          122 a          108 c           90 g          130 t
ORIGIN      
        1 atatatgttt cgattggtat aacggtgtct aaatattcct ctgaacatct tcctgataca
       61 aaaaaaaccg ccagcgtttc cgctggcggt tttcaatgta ttcagaactc agcaatcagt
      121 aaaaatcacc agtcgccttt tcggcctgat ccatccacac cggtttatcg ctggttttcg
      181 accagacgcg gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag
      241 gcagcgccag aacgcccgct gcaaccgcat aagtacggcg taaaaagata agccatgcag
      301 gatattctct gtagagttcc atacttttct cccccttttg tatctacccg gtgaatagca
      361 ccggaaaaat gaatttgttt atctgatgaa aatagtaccg ccttttgtgt aattttacta
      421 ctcatccgac cacttatttt tgcttattga
//
LOCUS       DAZUCC010000684          448 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZUCC010000684 DAZUCC010000000
VERSION     DAZUCC010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.687"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>448)
                     /locus_tag="ACHY09_005561"
     CDS             complement(<1..>448)
                     /locus_tag="ACHY09_005561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888334.1"
                     /translation="KSDIKKTLTLGATESLLPIFNTSTQVGYGGVIKVLPVFTLIQTT
                     IFSIPGVALFPAVIGTAILSGIVGSTSGGVGLVMVTFGQDLLELSQAQNISPGLMHRI
                     IVFSASTLDTLPHSGFIITLLGVCGLSHKQSYKELFIVTCVFPAFAV"
BASE COUNT          164 a           88 c           77 g          119 t
ORIGIN      
        1 accgcaaatg cgggaaacac acaagtaaca ataaaaagct ctttataact ttgcttatga
       61 gataagccac aaactcctag caatgtgata ataaagccag aatgaggaag cgtatccaac
      121 gttgatgcac taaaaacaat aatacgatgc attaaacctg gcgaaatatt ctgagcttga
      181 gatagttcta ataagtcttg accaaacgtt accataacta atcccacccc tcctgatgtt
      241 gagcccacta ttcccgaaag aattgctgta cctataactg caggaaatag tgctactcca
      301 ggaatagaaa aaattgttgt ttgaataagg gtaaacacag gaaggacttt aattacccca
      361 ccataaccaa cttgggtaga tgtattaaat attggtaaaa gagactctgt agcacctaat
      421 gtaagtgttt tctttatatc acttttaa
//
LOCUS       DAZUCC010000685          448 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZUCC010000685 DAZUCC010000000
VERSION     DAZUCC010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.688"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..>448
                     /locus_tag="ACHY09_005562"
     CDS             23..>448
                     /locus_tag="ACHY09_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS3888335.1"
                     /translation="MLKNLLNTKVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILS"
BASE COUNT          121 a          105 c          110 g          112 t
ORIGIN      
        1 atcatcggga gttatcagtc gcatgttaaa aaatctatta aatacaaagg tcgttcaggt
       61 cgtggaacag gtcaaagact ggcgggaagc cgtggcgatt tcatgccgac cgttgattga
      121 aaacggctct atcgaaccgc gttatgttga tgcgatctat cactctcacg acactatcgg
      181 tccgtactat gtggttggcc ctggtattgc gatgcctcat gcgcgcccgg aagagggggc
      241 taacaagctg tcattagcac tgaccctgat cccttcaggt gtgaattttg atgccgatga
      301 aaacgatcca gtgaaattac tgattgtgct ggctgcaacc gacagtacca gccacattga
      361 agcgatttct caactcgcca agttattcga taacgaaaaa gacatacagg ccattttgac
      421 ggccaaaacg acgcaggaca ttttgtct
//
LOCUS       DAZUCC010000686          441 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZUCC010000686 DAZUCC010000000
VERSION     DAZUCC010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.689"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /locus_tag="ACHY09_005563"
     CDS             <1..285
                     /locus_tag="ACHY09_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFS3888336.1"
                     /translation="RVVLLDAPEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQA
                     GLAEHYRALAHAWSLGLKKYPAVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            282..>441
                     /locus_tag="ACHY09_005564"
     CDS             282..>441
                     /locus_tag="ACHY09_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFS3888337.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWL"
BASE COUNT           90 a          110 c          137 g          104 t
ORIGIN      
        1 cgtgttgtcc tgcttgatgc tcctgagcag acgcaggata catttttcgg ggtacttccc
       61 gcagagcctg cagaagcatc tgcccgtgtc atggaacgga tgcagtcgcc tgaatggcag
      121 tcgtttcagg cagggcttgc tgaacactac cgtgcattag cacatgcctg gtcccttgga
      181 ctgaaaaaat atccggctgt ggtttttgat gacagtgaag tggtctacgg aaccacagac
      241 gtggtgctgg cagaacaact gcgtacaggg ggagggcagc catgatttcc tgcacatcac
      301 gtcatcgcgc cggtgttctg ctgttaagta tggcggtatt gccggtggca gaggcatcgc
      361 tgaacaccgc gcagatagtg gccagtgcag cctcacagag ctgtatcagc tggcgggtca
      421 gcgggatttg ctactggctg t
//
LOCUS       DAZUCC010000687          440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZUCC010000687 DAZUCC010000000
VERSION     DAZUCC010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.690"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>440)
                     /locus_tag="ACHY09_005565"
     CDS             complement(<1..>440)
                     /locus_tag="ACHY09_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888338.1"
                     /translation="SCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFPSPEYII
                     FSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGMAEGAMA
                     RTISQLSKGQVLSLTTSPTKENEPIFFSHGSHIHSYFICSPFFL"
BASE COUNT          135 a           71 c           88 g          146 t
ORIGIN      
        1 aagaaagaat ggggagcaaa taaagtaaga gtgaatatga gaaccatgac taaagaatat
       61 aggttcattt tcttttgttg ggctggttgt taatgataaa acctgtcctt ttgatagttg
      121 agaaattgtt ctggccatgg ctccttctgc cattccaaga caccataagc tcaatggctt
      181 atttcgatgg cttgcatatt tcataatagt cacacccagc ctgcattctt cttctttgct
      241 gtatggaata ctgcaaaagt agtggttaac aaatggacca ctgtttttcg taagatagtc
      301 agaaaaaatt atatactcag gagatgggaa aagcctgtta tcaattttta catgcttcaa
      361 aggtttatgt tttactccag atgctgttga tgagaaaaaa tcagccattt tgttaattct
      421 tggtgctgca taacatgatt
//
LOCUS       DAZUCC010000688          435 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZUCC010000688 DAZUCC010000000
VERSION     DAZUCC010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.691"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>435
                     /locus_tag="ACHY09_005566"
     CDS             <1..>435
                     /locus_tag="ACHY09_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFS3888339.1"
                     /translation="HPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFE
                     DAPEIIQSGLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHM
                     PFIGSVGPAFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRE"
BASE COUNT           96 a           91 c          132 g          116 t
ORIGIN      
        1 atcatccgga atttcagaaa gtggagtccc tgtggcgggg cgtgaagtct ctggtggata
       61 aaacggactt ccgccgtaat gtgaaaattg aactgctgga tctgtccaaa gacgatctgc
      121 gtcaggattt tgaggatgcg ccggaaatta ttcagagtgg tctttatctg cagacttatg
      181 tggcggagta cgatacgccg ggtggtgaac ccattgcagc cctgatttct gcctgggagt
      241 ttgatgcgtc tgcgcaggat gtggcgctgc tgaaaaatat ttcccgggtg gctgcgtccg
      301 cgcatatgcc atttattggt tcagttggcc cggctttctt ccggaaggat acgatggaag
      361 aggtggccgc cattaaggat attggcaatc actttgagcg tgccgagtac atcaagtgga
      421 atgctttccg tgaaa
//
LOCUS       DAZUCC010000689          435 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZUCC010000689 DAZUCC010000000
VERSION     DAZUCC010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.692"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>435
                     /locus_tag="ACHY09_005567"
     CDS             <1..>435
                     /locus_tag="ACHY09_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888340.1"
                     /translation="GAEFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQ
                     SNGMSYGEGLPLPDTFDSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMT
                     GLNHPKADLSKGQYGCVGQGLHIAKKLLPFIPANAGILLVPCC"
BASE COUNT          100 a          108 c          121 g          106 t
ORIGIN      
        1 ggtgcggagt tcatccagcc agttgtgagt gaggggagca tctcatcacc agaggagcca
       61 ggcaaccgtc cgacgacctc agcgccttct gttgcgccag aatattacta tgtgatcgcg
      121 cttgctggtc agtccaatgg tatgtcatac ggtgagggac tgccattacc ggatacattc
      181 gacagccctg atccacgtat taaacagtta gcgcgtcgca gtacggtgac accgggaggt
      241 gctgcatgta tctataacga tattatcccg gcagatcact gtctccatga tgtggaggat
      301 atgacagggc ttaatcatcc caaagcggat ttatcgaaag gtcagtacgg ctgtgtggga
      361 caggggctgc atatcgccaa aaagcttctg ccatttatac cggcgaatgc gggcattctt
      421 cttgttccgt gctgc
//
LOCUS       DAZUCC010000690          434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZUCC010000690 DAZUCC010000000
VERSION     DAZUCC010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.693"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(38..316)
                     /locus_tag="ACHY09_005568"
     CDS             complement(38..316)
                     /locus_tag="ACHY09_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888341.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPEKGAEISVTEE"
BASE COUNT          104 a          132 c           80 g          118 t
ORIGIN      
        1 accgggtgct gaaaaaaact gcaccgacgg cgctctttta ctcctccgtt acggatattt
       61 cagccccctt ttcaggagcg gtatccttca cctcgctgag cccggactga gccgcttcag
      121 aaacctctgc cacctttccg gcagcctgca ttttcacttc ctccgcggca tccttcacat
      181 cactgagttg agtctgcgca gcttccttta tatctgccgc cttcttactt acattctcct
      241 ttgtcgcatc atcacagccc gcaaccagaa atggtgcgga aatacataac gccagaattg
      301 ttttttcagc atacaatctc tcattattaa gtgttaacgc agaacagctt atatttcgtc
      361 cccgggaaat ggtcaaaaga caaccagaac atcacagtgt cagggcacac tcccggtata
      421 tgcttttata acca
//
LOCUS       DAZUCC010000691          434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZUCC010000691 DAZUCC010000000
VERSION     DAZUCC010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.695"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(57..293)
                     /locus_tag="ACHY09_005569"
                     /pseudo
     CDS             complement(57..293)
                     /locus_tag="ACHY09_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          119 a           95 c           81 g          139 t
ORIGIN      
        1 tccagatcac tggcggaacc tttggtcata tccagtgcac cattttgcaa caactacata
       61 gtttctcgcg cattacccat ctggctgctg gggtaaatac ggatcaccat attacctttt
      121 gacagctgtt ttacttcttt cgccagctct tcaaaactct gatgtacaac atgacttctt
      181 tccaggttgt gtgctaactt taacgtcact ttttctgcgg cgtttacgcc agtgctaacc
      241 agagccagta aggatgcgca gactaatgag cgggtaaatg acctcatttt cataaaaaaa
      301 ctccctgttg aagaatggaa attggttgat atctaattgt gcttatcagt gctttgtgcg
      361 acaaattggt taacctgtct gacaacttgt ctatgtttat cacaacttga aataaagtca
      421 gaaattaaat atta
//
LOCUS       DAZUCC010000692          428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZUCC010000692 DAZUCC010000000
VERSION     DAZUCC010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.696"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>428
                     /locus_tag="ACHY09_005570"
     CDS             <1..>428
                     /locus_tag="ACHY09_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFS3888342.1"
                     /translation="LKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVS
                     GDITDITTPDGRETKFYYNDGNQLTAVVYPDGLESSRAYDELDRLVSETSRSGETVRY
                     RYDDVHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGY"
BASE COUNT          101 a          112 c          157 g           58 t
ORIGIN      
        1 gctgaagcgg gtggtgaaaa aagaactggc ggacggcagc gtcacgcaca gcgggtatga
       61 cgcggcagga aggctcacgg cacagacgga cgcggcggga cggcggacag agtacggcct
      121 gaatgtggtg tccggcgata tcacggatat caccacgccg gacgggcggg agacgaaatt
      181 ttactataac gacgggaacc agttaacggc ggtggtgtac ccggacgggc tggagagcag
      241 ccgggcatat gatgagctgg acagactggt atcggagaca tcgcgcagcg gggagacagt
      301 acgctaccgc tacgatgacg tgcacagtga gttaccggcg acgacaacgg atgcgacggg
      361 cagcacccgg cagatgacct ggagccgcta cgggcagttg ctggccttca ccgactgctc
      421 gggctacc
//
LOCUS       DAZUCC010000693          425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZUCC010000693 DAZUCC010000000
VERSION     DAZUCC010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.697"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..424)
                     /locus_tag="ACHY09_005571"
     CDS             complement(<1..424)
                     /locus_tag="ACHY09_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFS3888343.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARSAEH"
BASE COUNT           94 a          137 c           91 g          103 t
ORIGIN      
        1 tgtgttctgc agagcgtgca gacgccgctg tgccagcctg actgaccgta ctgacgcttc
       61 cctcatacac aatccgtacg ggctctgtgg gccctttttc tgctgctgtc acatccagca
      121 gaataatttc tccgctctcc acatcctgca gttgcagacg cgtctgcgga aaaacactct
      181 ctgctttcag atagactgcc ccaccggtac tctgtaccct caactttccg ttcagtgctg
      241 gcgggaagcc cacccgcaca tttttatctg caaaaataac ccgctcctgt cctgccatca
      301 gggtaacggg taagggaatg cgctcccatt tcatcagctc atcagcccgt gcgctcagtg
      361 gtaaaaccag cagtgacagg accatcacaa tactacggta taaacaacgt tcagctccgt
      421 tcatc
//
LOCUS       DAZUCC010000694          421 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZUCC010000694 DAZUCC010000000
VERSION     DAZUCC010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.698"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          135 a           66 c           93 g          127 t
ORIGIN      
        1 ttatctgtag gacaaatatg gagcgcttga tgagtcagag ctaaaacagc aattgataaa
       61 taaacatgtt gatttattca agaaaatgga tgtatttcaa atattgattc ttaatataac
      121 ctaatcaata gattatcacg tgagagctgc acattattgg aggggcaggg gaaaacacca
      181 ttttgggaac aatgaatcag gtactcttac cactgaagca agatcatccg ggcaccaggt
      241 ggtggtgcag tgctttggaa atggtcccct cgggggagtt gtcattattt tctatgctat
      301 ttgcttatga tattcatcag gtgaatttaa taaattccta attttaaata tccactatta
      361 agccagtgtt taacggcgtt aagatggcaa tgtgcagatc atgttacagg gaaagatcac
      421 g
//
LOCUS       DAZUCC010000695          416 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZUCC010000695 DAZUCC010000000
VERSION     DAZUCC010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.699"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>416
                     /locus_tag="ACHY09_005572"
     CDS             <1..>416
                     /locus_tag="ACHY09_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3888344.1"
                     /translation="PQNPELIYCWEQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHVNH
                     LLLSYSKQSLISRCYNLLLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILD
                     DVRLNNALSAIQTTVKPISEIARENGYKCPSRFTER"
BASE COUNT          128 a           80 c           83 g          125 t
ORIGIN      
        1 tacctcagaa tcctgagtta atatattgtt gggagcaact aaaaacatct gtttcccgag
       61 gcttctcaac taaaattcag gagcacttag caatgggcgt tctactttcg ttaggagtga
      121 atcatgttaa tcatttactt ttatcatata gtaaacaatc attgataagt cgttgttata
      181 acctgctgct atccgaaccc ggcacaaaat ggacagcaaa caaggttgct cgatatctct
      241 acatttctgt ttctacatta catcgccgtc tagcaagcga gggggtaagt ttccaaagta
      301 tactggacga tgtgaggtta aataatgcgt tgtctgctat acaaacgacg gtaaaaccta
      361 taagcgagat tgccagagaa aatggttata agtgtccttc tcgttttact gaaaga
//
LOCUS       DAZUCC010000696          409 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZUCC010000696 DAZUCC010000000
VERSION     DAZUCC010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.700"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..161)
                     /locus_tag="ACHY09_005573"
     CDS             complement(<1..161)
                     /locus_tag="ACHY09_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFS3888345.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTR"
     gene            complement(167..>409)
                     /locus_tag="ACHY09_005574"
     CDS             complement(167..>409)
                     /locus_tag="ACHY09_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3888346.1"
                     /translation="LSLKRAEAVRDWMRDTGDIPQSCFAVQGYGESRPVAPNDSAEGR
                     ARNRRVEISLVPQADACRLPDASPAPEEGASENITE"
BASE COUNT           86 a          135 c          103 g           85 t
ORIGIN      
        1 cgggtaccgg tcagcttacc ggtattgtta tccgtcggaa tgtaaaggca gtgctcctga
       61 gccaccacct caatgctgcc ctcacggtcc ttcacatcca cggagccttt aatatccgct
      121 ccgccatcat cctgcagcca cagataaaca ggaattgcca taaaaatcac tccgttatgt
      181 tttctgatgc gccctcttcc ggcgcagggg aagcgtccgg cagacgacag gcatcggcct
      241 gcggtaccag actgatttca acacggcggt tacgcgcgcg gccttccgcc gagtcattcg
      301 gggccacagg gcggctttca ccgtatccct gcacggcaaa acagctttga gggatatcgc
      361 ccgtatcccg catccagtca cgaaccgcct ctgcacgctt cagggacag
//
LOCUS       DAZUCC010000697          407 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZUCC010000697 DAZUCC010000000
VERSION     DAZUCC010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.701"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..>407
                     /locus_tag="ACHY09_005575"
     CDS             56..>407
                     /locus_tag="ACHY09_005575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HFS3888347.1"
                     /translation="MIFAEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVAS
                     PEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDW
                     KKVGSLPEGLAYGVS"
BASE COUNT          116 a           75 c          111 g          105 t
ORIGIN      
        1 aaaccagtgg aagaacctgg gaacgactcc atttccggga actgctggtt catcggtgat
       61 ttttgcagaa cagcaaattt atatactggg aggggagaga aaaccgggcc ttcgttctgt
      121 tagatcctgg acgggggagc tttcgcatga tcgaattaaa tggtctgaac ttccaccagt
      181 agcttcaccc gagggggtgt caggagcata tgcctctgtt attgatggaa atatctttct
      241 ggccggtggt gcatatttcc caggggcggc agaaaagtat agcaatggtg aatactggtc
      301 tcataaagga ctggataaag cctattcaaa agagatatat cagctaataa aaaatgactg
      361 gaaaaaagtt ggttctttgc cggaaggact tgcttatggt gtaagcc
//
LOCUS       DAZUCC010000698          407 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZUCC010000698 DAZUCC010000000
VERSION     DAZUCC010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.702"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>407
                     /locus_tag="ACHY09_005576"
     CDS             <1..>407
                     /locus_tag="ACHY09_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFS3888348.1"
                     /translation="PDRPYIAYALHDSEHPDHVTSDNHTRNVWRTPANNKLRMEDKRQ
                     EEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTDEHGAVRAAKGLFLTADAQARA
                     QGPVLEMAAAISQINQANSQMQALNSAAEAAGAL"
BASE COUNT          106 a          112 c          127 g           62 t
ORIGIN      
        1 ccggatcgtc cctacatcgc ttatgccctg catgattcag agcatccgga tcatgtcacc
       61 agtgacaatc atacgcggaa cgtgtggcgc acgccggcga acaataagct gcggatggag
      121 gataagcgcc aggaagagca tatcaagctc gcaacggaat atggcaaaac acagctgaac
      181 atgggacacc tggtcaacag ccggcgtgaa aagcgaggtg ccggttttga actgcgaacg
      241 gatgagcatg gggctgtacg tgcagcaaaa gggctgttcc tgacggcaga tgcccaggcc
      301 agagcccagg ggccggtgct ggaaatggcg gccgcgataa gtcagattaa ccaggctaac
      361 agccagatgc aggccctgaa cagcgctgcc gaagccgccg gcgccct
//
LOCUS       DAZUCC010000699          404 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZUCC010000699 DAZUCC010000000
VERSION     DAZUCC010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.703"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>404
                     /locus_tag="ACHY09_005577"
     CDS             <1..>404
                     /locus_tag="ACHY09_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFS3888349.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQI
                     GTFAINFSRPAGQVIAQYYEFLRLGREGYTKV"
BASE COUNT           76 a          121 c          115 g           92 t
ORIGIN      
        1 ttcggcgtga cctacaccgg taactatgag ttcccgcagc cgctgcacga tgcgctggat
       61 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
      121 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      181 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      241 tggcgtgatg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggcggt
      301 cagattggta ctttcgccat caacttctcc cgcccggcgg gtcaggtgat tgcacagtac
      361 tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag taca
//
LOCUS       DAZUCC010000700          402 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZUCC010000700 DAZUCC010000000
VERSION     DAZUCC010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.704"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>402
                     /locus_tag="ACHY09_005578"
     CDS             <1..>402
                     /locus_tag="ACHY09_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888350.1"
                     /translation="DDGSVSVGGTRMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGV
                     NDQDAVNVSQLNREISQATAATTWALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKD
                     DKGNYSYEIDVTGIPVEYTDSQGNPLVNIGGR"
BASE COUNT          103 a           90 c          126 g           83 t
ORIGIN      
        1 agatgacggc agtgtttctg tcggcggaac ccggatggat aaaaacggac tgaccatcaa
       61 ggatggcccg tcagtgactg tgaacggtat tgatgcggct ggccgggtta tttccggcgt
      121 gaaagacggg gtgaatgacc aggacgccgt taacgtttca caactgaacc gtgaaatcag
      181 tcaggcaacg gcagcaacga cctgggcact gaagactgaa tccggtgatg aggctgttgc
      241 tgtttcttcc cagacagttg aagtccgcca cggacagaat acccgggttt cagccatcag
      301 taaggatgac aagggaaatt acagctatga gattgatgtg acggggattc cggtggagta
      361 caccgacagc caggggaacc cgcttgtcaa tattggtggt cg
//
LOCUS       DAZUCC010000701          394 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZUCC010000701 DAZUCC010000000
VERSION     DAZUCC010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.705"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>394
                     /locus_tag="ACHY09_005579"
     CDS             <1..>394
                     /locus_tag="ACHY09_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFS3888351.1"
                     /translation="QPVKYAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGK
                     ITTLYPSEPLTVTPLAAWAAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRW
                     LGAAGTPEDTTTLYVVTAERPEQAFIAEP"
BASE COUNT           84 a          116 c          115 g           79 t
ORIGIN      
        1 tcagccggtg aagtatgcag agcctttacc cgttctgttg acccgctatg cggcacagtc
       61 gctgtacgca ccgttacgga cgctggaagc ggtaccgggt atccggccgg ttaatacaca
      121 tctgcccggg aaaatcacca cactctatcc gtcagagcca ctgacagtca cgccactggc
      181 agcctgggct gcgggtgggc gtgcagttgt tgcactgaaa ctgacgagta cggtaagccg
      241 caaaattatc cttgatccgc gggcacttca gggaaaattc attgccgcga ctttccagca
      301 ccgctggctg ggtgcagcag gtacccctga ggataccacc acgctctatg tggtgacggc
      361 agaacggccg gaacaggcgt ttatcgcgga gcct
//
LOCUS       DAZUCC010000702          387 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZUCC010000702 DAZUCC010000000
VERSION     DAZUCC010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.706"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>387
                     /locus_tag="ACHY09_005580"
     CDS             <1..>387
                     /locus_tag="ACHY09_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888352.1"
                     /translation="FFLGGRCYTAENLEKDYLAEVANYSDDRWEAPQRAARLAAAVKR
                     YKTSEMLRFIFATTAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHRSA
                     DSNPEMVEKMAARYRHAAELHWSATL"
BASE COUNT           94 a           94 c          112 g           87 t
ORIGIN      
        1 ggtttttttt aggcggcagg tgttacacgg ctgaaaacct tgaaaaggat tatctggctg
       61 aagttgctaa ttacagtgac gaccgctggg aagcacccca acgcgccgca cgtctggcag
      121 ctgccgtaaa gcgttacaag acatcagaaa tgctgcgctt tatttttgct accactgcct
      181 acgatcccga tccagattta accccgctga ctgtgagacg tttgtgcaag gcattgttcg
      241 gcagaacagg aagccagtgg ctggttgttg aagtttttgg ggagaagggg cgtcagcacc
      301 gcagcgcgga cagcaatccg gagatggtgg aaaaaatggc tgccagatat cgtcatgccg
      361 ccgaactaca ctggtccgca acgctgg
//
LOCUS       DAZUCC010000703          386 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZUCC010000703 DAZUCC010000000
VERSION     DAZUCC010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.707"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..309
                     /locus_tag="ACHY09_005581"
     CDS             106..309
                     /locus_tag="ACHY09_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm52 family immunity protein"
                     /protein_id="HFS3888353.1"
                     /translation="MPHILLPELIPEAAKIVPVFEGEKQKGTIVVSTEDVFDGNNKEH
                     IGKANDIEIRLLDLGLLPLLTEL"
BASE COUNT          125 a           72 c           77 g          112 t
ORIGIN      
        1 ctaaaaaact aaatcttatc atagatattg atcagagagt tgccaatact tcggatatga
       61 ttgattcctt gcttctgttg gctgaatgtg ggatggatgt tatatatgcc ccacatactc
      121 ttacctgaat taatccctga agccgcaaaa atagtaccgg ttttcgaggg cgagaagcaa
      181 aagggaacta ttgttgtttc aaccgaagac gtatttgacg gaaataataa ggaacacatc
      241 ggaaaagcta atgatattga aataagactt ctggatcttg gactgctacc tttgctaaca
      301 gagctttaat ccaaccttat cactcatggg tagatgacgg gacagttaca cccgttaatg
      361 gccttatttt taacacatca gataaa
//
LOCUS       DAZUCC010000704          386 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZUCC010000704 DAZUCC010000000
VERSION     DAZUCC010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.708"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>386
                     /locus_tag="ACHY09_005582"
     CDS             <1..>386
                     /locus_tag="ACHY09_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3888354.1"
                     /translation="AWLKEKGAFDQPYWMMKASLPAVLNKVITDVGPGKLNLGGCVIE
                     VMGTYNAAIVRVTIGEYGATGFINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRD
                     ASTTQKGVVQLSSDTGSNDETKAATP"
BASE COUNT          109 a           86 c          116 g           75 t
ORIGIN      
        1 gcctggctga aagaaaaagg ggcatttgac cagccttact ggatgatgaa ggcatcactg
       61 cctgcggtac tcaataaagt catcacggat gtcggaccgg gaaaactcaa tctgggtggc
      121 tgtgtcattg aggtgatggg gacgtataac gcagccatag tccgggtcac cattggcgaa
      181 tacggtgcaa cggggtttat taatggcacg gtctgtacct gtacggttta cggcgacaca
      241 cagtatttcc actggcgggt ggattacagc acaaaaaaca aaccggatac ggtcagccag
      301 cgggatgcca gcacgacgca gaaaggtgtg gtgcagttaa gcagtgatac tggcagtaat
      361 gacgaaacaa aggcagccac gccgaa
//
LOCUS       DAZUCC010000705          385 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZUCC010000705 DAZUCC010000000
VERSION     DAZUCC010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.709"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>385
                     /locus_tag="ACHY09_005583"
     CDS             <1..>385
                     /locus_tag="ACHY09_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFS3888355.1"
                     /translation="NKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRP
                     WSMASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLVVDKIHVDLNWRYLNEY
                     LDHISANDEVLFLKQGHEIIAKNQLA"
BASE COUNT          115 a           74 c           84 g          112 t
ORIGIN      
        1 ataataaata ttatctcttt tcgcggaggg cgaatgataa aatcgttgtt tatcatcagg
       61 aacaagtaaa tggaccgctt gtcgacgagt cagggcgggt tatttttgcc gattttaacc
      121 catcgaaacg accgtggtcg atggcttcag atgactctaa caacagctgg aatccggcat
      181 acaattgctt tgatcgtccg ggtaaaaaat gtatctcttt tacgctacgc atcaacggca
      241 aagatcacga tttgttagtg gtggataaaa ttcatgtcga tttaaactgg cgatatctga
      301 acgagtatct tgatcacatc agcgctaatg atgaagttct atttttaaag caaggtcatg
      361 agatcattgc caagaatcaa ctcgc
//
LOCUS       DAZUCC010000706          382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZUCC010000706 DAZUCC010000000
VERSION     DAZUCC010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.710"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            62..>382
                     /locus_tag="ACHY09_005584"
     CDS             62..>382
                     /locus_tag="ACHY09_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888356.1"
                     /translation="MTQSRQPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVV"
BASE COUNT           72 a          123 c          130 g           57 t
ORIGIN      
        1 agctcattga cgccgacttt cagcgcctca gaaacctcat ctggccggag gatgacaggt
       61 aatgacccag agccgccaac cgtcgccact gcagcggaga gtgctgattg tgctggctgc
      121 ccttgatgaa aaacggccag gccccgtgct gacgcgggac atagaacggg tgctggagcg
      181 aagcggggaa gccccggtct acgggccaaa cctgcgcgcc tcctgccgtc gtctggagga
      241 tgccggctgg ctgcgtaccc tgcgcgctcc gaacctgcag ctggcagtgg agctgacgga
      301 cgccggccgg gccgtggcac agcctctgct gctggcagaa caggaccgcc tccgggcaga
      361 acagcgtgcg gcagaagtcg tc
//
LOCUS       DAZUCC010000707          382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZUCC010000707 DAZUCC010000000
VERSION     DAZUCC010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.711"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          137 a           73 c           82 g           90 t
ORIGIN      
        1 gataattaat gtcactctgt atggatgaaa aaactgacag cagaagtctc cgctgacaaa
       61 gacaggaatg atgattaatg tcaacttggc cagtgagcca gccccttaaa acgccagaca
      121 gtggatgtat attaaaataa gagataggct gggaaaatat aaagagtaac tgcgctttct
      181 gagtaaaaaa acaacagcag caggtacttc aaaaccgtga tgcactgtca gttgcaaaga
      241 cgaatgtaca gttacctttg ttaccagagg acaagaagtg ccccgggtat taccgtgaaa
      301 gaaatcagtg aacatttatt gaccataacc acattgcttc tccgtagact gttgatttga
      361 aagagttaac atccccgtaa aa
//
LOCUS       DAZUCC010000708          378 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZUCC010000708 DAZUCC010000000
VERSION     DAZUCC010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.712"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>378
                     /locus_tag="ACHY09_005585"
     CDS             <1..>378
                     /locus_tag="ACHY09_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFS3888357.1"
                     /translation="GWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIR
                     YRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEGGLKRVVKKEHA
                     DGSVTQSQFDAVGRLKAQTDAAGR"
BASE COUNT           93 a          103 c          131 g           51 t
ORIGIN      
        1 ggctggacgc cgcgcggcga actggcggcg gtgtatgacc gcagcaatac acaggtgcgc
       61 agctttactt acgatgataa ataccggggc cggatggtgg cgcaccgtca cacgggccgg
      121 ccggaaatcc gttaccgtta cgacagtgac gggcgggtga cagaacagct aaacccggca
      181 ggcttaagct acacgtatca gtatgagaaa gaccacatca ccatcaccga cagcctggac
      241 cgccgtgaag tgctgcacac gcagggcgaa ggcgggctga agcgggtggt gaaaaaggaa
      301 cacgcggacg gcagcgtcac gcagagtcag tttgacgcgg tgggcaggct taaggcacag
      361 acggatgccg caggccgg
//
LOCUS       DAZUCC010000709          374 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZUCC010000709 DAZUCC010000000
VERSION     DAZUCC010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.713"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..>374
                     /locus_tag="ACHY09_005586"
     CDS             38..>374
                     /locus_tag="ACHY09_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFS3888358.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     NAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGAD"
BASE COUNT          116 a           79 c           91 g           88 t
ORIGIN      
        1 gaaaacaatc cgctatgctt tgaaaaagga gaaagaaatg atgaaaaagt ttatcgcccc
       61 cttgttggct ttactggtta gcggatgtca gattgatcct tatactcacg cgccaacctt
      121 gaccagcacc gactggtatg atgttggtat ggaagatgcg atatcgggca acgccataaa
      181 agatgacgat gcatttagcg attcacaggc ggatcgcggt ctatacctta aaggatatgc
      241 cgaaggacaa aagaaaactt gccagaccga ttttacttat gcccgaggac tttccggtaa
      301 aagctttcct gccagctgca acaatgttga aaatgccagc caactgcatg aagtatggca
      361 aaaaggggct gatg
//
LOCUS       DAZUCC010000710          373 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZUCC010000710 DAZUCC010000000
VERSION     DAZUCC010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.714"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>373
                     /locus_tag="ACHY09_005587"
     CDS             <1..>373
                     /locus_tag="ACHY09_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFS3888359.1"
                     /translation="VKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAGL
                     QPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGHP
                     GFSFFNRGGDVLTFDPLNKLDR"
BASE COUNT           78 a           90 c          128 g           77 t
ORIGIN      
        1 gtgaagaaat atctgggaaa ccggcacaag ctctaccgtg ccgggatcac gtttctgctg
       61 cgggcagagg atatggagag tctgaagcgc agacgggttg aactgacgac ggtgctgctg
      121 ggcgcgggct tacagccagt gcgcccggag tttgatgtcg gaccgctgaa cagctggctg
      181 cgcgcgctgc ccatgtgttt tgatcctgat acggataaaa aacagtggta cacgcgtctg
      241 atgtgggtac agcatctggc agggctcctg ccggtgacgg gaagggaaac cggtacgggg
      301 catcccggat tcagtttctt taaccgtggc ggggatgtgc tgacctttga tccgctgaac
      361 aagctggacc gga
//
LOCUS       DAZUCC010000711          369 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZUCC010000711 DAZUCC010000000
VERSION     DAZUCC010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.715"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            37..>369
                     /locus_tag="ACHY09_005588"
     CDS             37..>369
                     /locus_tag="ACHY09_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS3888360.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGN"
BASE COUNT           76 a           76 c           82 g          135 t
ORIGIN      
        1 tagactttcg tcacttttaa ttaaagggat gtttttatgc agtggtattt agccgtatta
       61 aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag agtactggat gtttactctg
      121 attaacgcca tcgtcggcgc tattatcaat gtcattcaat tgattttagg tttagagttt
      181 ccattccttt ctctcattta cctggcagca acaatcattc cggtcattgc tctgtgcgtt
      241 cgtcgtctgc acgatactga ccgttcaggt gcgtgggcgc ttctctatct ggttccgatc
      301 attggctggt tagttctgtt tgtatttgcc tgtttagaag gcaactctgg cagcaatcgt
      361 tacggaaac
//
LOCUS       DAZUCC010000712          367 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZUCC010000712 DAZUCC010000000
VERSION     DAZUCC010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.716"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..286)
                     /gene="fumD"
                     /locus_tag="ACHY09_005589"
     CDS             complement(77..286)
                     /gene="fumD"
                     /locus_tag="ACHY09_005589"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFS3888361.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          105 a           94 c           57 g          111 t
ORIGIN      
        1 gttatttaga atgattaact ggaccgcaac tgaagcaaga atatcaggat gaagcttacc
       61 gcctcatcct gaatggtcat tttaccagtg cgttaatgac ggtttccatc gcctgctttt
      121 ccagctcact gcgctgaatg cgtttattgg ctaatgctgt acgtaatacg cctgcgataa
      181 caatatgctt tggttcctgc cccagatcgc gcatttccag cacaacttta ccgacaactc
      241 tgcacatttc tcggtacagt tcatcctctt ttgtccgatt tcccattctt tatacctatt
      301 tatcatgcca actatcagca tatatcaatc taaccaatta aacaaaaatc aaacaaaatc
      361 agacaaa
//
LOCUS       DAZUCC010000713          366 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZUCC010000713 DAZUCC010000000
VERSION     DAZUCC010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.717"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..229
                     /locus_tag="ACHY09_005590"
     CDS             <1..229
                     /locus_tag="ACHY09_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3888362.1"
                     /translation="KRADAGIAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAGFH
                     FNTSENSAVKVNAAWDTENGAGVSAGMALGW"
BASE COUNT           91 a           82 c          110 g           83 t
ORIGIN      
        1 aaaacgtgca gatgcaggta ttgcaggtgc gatggcaatg acggggatac cgtccgtccc
       61 cgggaaaaag gtctcatttg gtatggccat ggccggatac cggagtgaag gtgctgttgc
      121 tgccggcttc cattttaata caagtgaaaa cagtgctgtt aaagtgaatg cagcctggga
      181 cactgaaaat ggagctggcg tttcagccgg gatggctctg ggatggtaat gcttccgggc
      241 cagaagccgt tcgcagatat tgttatccag gggacactgc accaccattc aggaagcgat
      301 tcagtttatc gcatcggctc aggatgtaat taatacggac aatctggctc ctgcaccgga
      361 gccaga
//
LOCUS       DAZUCC010000714          361 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZUCC010000714 DAZUCC010000000
VERSION     DAZUCC010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.719"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..>361
                     /locus_tag="ACHY09_005591"
     CDS             3..>361
                     /locus_tag="ACHY09_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS3888363.1"
                     /translation="MAAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGATRVDG
                     KPVIPGMKLSKEKCDQVNAIERDKALAWVAKNIRVPLTEPQKAGIASFCPYNIGPGKC
                     FPSTFYKRINAGDRRGA"
BASE COUNT           89 a           88 c          110 g           74 t
ORIGIN      
        1 tgattgccgc aggggcttct gcgcctgaaa tcctcgacca gtttctggat gaaaaggaag
       61 gcaaccacac cacggcatac cgtgatggtg cgggtatctg gaccatctgc cgtggagcca
      121 cccgggtgga tggtaagcct gtgattcctg gcatgaagct gtcgaaggaa aaatgcgacc
      181 aggttaacgc cattgaacgg gataaggcgc tggcatgggt ggcgaaaaac atcagagtgc
      241 cactgaccga acctcagaaa gcggggatcg cgtcattctg tccttacaac attggccccg
      301 gtaagtgttt cccgtcgacg ttttataaac gaattaatgc aggcgatcgc aggggagcgt
      361 g
//
LOCUS       DAZUCC010000715          358 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZUCC010000715 DAZUCC010000000
VERSION     DAZUCC010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.720"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>358
                     /locus_tag="ACHY09_005592"
     CDS             <1..>358
                     /locus_tag="ACHY09_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFS3888364.1"
                     /translation="DDKWALIPGIQTQWMSAGSQYNPYLKISYQATPDLNLALRYRYD
                     WKAFRQTDLDGNRARNDQHQFDGYVTYKVNDSWLFAWQTTVYTKVNNFKYGNHKKTAT
                     ENAFVLQYKMSPVFTPY"
BASE COUNT          117 a           67 c           77 g           97 t
ORIGIN      
        1 agatgacaaa tgggctctaa tcccagggat tcagacacaa tggatgtcag caggaagtca
       61 gtataaccct tatctgaaaa tttcgtatca ggcaacgcca gatcttaatc tggccttacg
      121 ctatcgctat gactggaaag ctttccgaca gacggattta gatggcaaca gggcgcgtaa
      181 tgatcagcat cagtttgatg gttatgtgac atacaaagtt aatgattcat ggttgtttgc
      241 atggcagacc acggtatata cgaaggttaa taatttcaaa tatgggaatc ataaaaaaac
      301 agcaactgaa aatgcatttg ttctgcaata taaaatgtca ccggtgttta ccccatat
//
LOCUS       DAZUCC010000716          356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZUCC010000716 DAZUCC010000000
VERSION     DAZUCC010000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.721"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..>356
                     /locus_tag="ACHY09_005593"
     CDS             20..>356
                     /locus_tag="ACHY09_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS3888365.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVI"
BASE COUNT          121 a           51 c           78 g          106 t
ORIGIN      
        1 tacgttagca ggtgtcacca tgaaaaaagc catagcatat atgcgatttt catcaccagg
       61 tcagatgtct ggcgactcat taaaccgaca gagaagactt attgctgaat ggttaaaggt
      121 aaatagtgat tattatcttg ataccataac atatgaagat ttaggattaa gtgcattcaa
      181 aggaaagcat gcacaatcag gagctttttc ggaattttta gatgctatag agcatggtta
      241 tatattgcca ggaactacat tgttagttga aagtctggac agactttcaa gagaaaaagt
      301 cggtgaagcg attgaacgtc tgaaattgat tttgaatcac ggtattgatg ttataa
//
LOCUS       DAZUCC010000717          353 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZUCC010000717 DAZUCC010000000
VERSION     DAZUCC010000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.722"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..300)
                     /gene="tssD"
                     /locus_tag="ACHY09_005594"
     CDS             complement(<1..300)
                     /gene="tssD"
                     /locus_tag="ACHY09_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFS3888366.1"
                     /translation="MSDIVYLRIIGEKQGYISSGCGTYASVGNCWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWER"
BASE COUNT          104 a           84 c           58 g          107 t
ORIGIN      
        1 ccgttcccag cgcccgcttt tatttatacg atacaaatcg atttcaataa ataaccgctc
       61 attctgattg atggcattag acagtagcgg tgagctttta tcaatgagtt tgcaaaattg
      121 tagcccctgt agattaacgc ctttaccagt actggtaatg gcgttggtga gtgcaaaggc
      181 aaaaatttca tcttcatggc caacctgcca gcaattacca accgacgcat acgtcccaca
      241 gcctgaagaa atatagccct gcttctcacc gattattctc aggtaaacaa tatcgctcac
      301 tttatttcat tccagtttat ccttaactta tttgcattaa atagaaaact taa
//
LOCUS       DAZUCC010000718          349 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZUCC010000718 DAZUCC010000000
VERSION     DAZUCC010000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.723"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..299)
                     /locus_tag="ACHY09_005595"
     CDS             complement(<1..299)
                     /locus_tag="ACHY09_005595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888367.1"
                     /translation="MTTCSELKERARFDGMHDASLEVAWQLLMTKSVSTITTEIISGA
                     DMSNFSVIIRERYKHAIAKSLLNHLSPDVVSDIIGLSHDEFNQQYKGNLTRQQHL"
BASE COUNT          103 a           59 c           72 g          115 t
ORIGIN      
        1 ctcaggtgtt gttgtctggt taaattacct ttatattgtt gattgaactc atcatgagat
       61 aaaccgatga tgtcagaaac aacatcggga gataaatgat tgagaagaga ttttgctatt
      121 gcatgtttat atcgttccct gataatcact gaaaaattgg acatgtctgc accggatatt
      181 atctcagtag tgatagtgga tacacttttc gtcataagta attgccatgc cacttcaaga
      241 gaagcatcat gcataccatc aaaacgggct ctttccttca gttcagaaca tgttgtcatt
      301 gaactcacct gcgaattttg tgatgtgtgt ataattataa agtgggggt
//
LOCUS       DAZUCC010000719          347 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZUCC010000719 DAZUCC010000000
VERSION     DAZUCC010000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.724"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(144..285)
                     /locus_tag="ACHY09_005596"
                     /pseudo
     CDS             complement(144..285)
                     /locus_tag="ACHY09_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT           91 a           82 c           86 g           88 t
ORIGIN      
        1 gagattattc ttacggggcc gggcgaatgc tatctgaatc gtacggttca tagaaataaa
       61 tatttaccga catagccgga gggttttcag atgcaatccc tgagtcactg cgaaatcgca
      121 tcgtatccga tcgcggactc gccgattctc agaacaggca gtccctgaga atcgcagaac
      181 gctagtgcac gatcgttgaa cacatcagct acggtgatcg atgttttact cgtattatag
      241 tttgcagtgt gccactttcg gggatgtagc tacgaacgtc tgctggcttc ccgatatgtc
      301 agtaaaaaat acccgccgta ttacagcaac ggcaggtaat ggcacat
//
LOCUS       DAZUCC010000720          345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZUCC010000720 DAZUCC010000000
VERSION     DAZUCC010000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.725"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>345
                     /locus_tag="ACHY09_005597"
     CDS             <1..>345
                     /locus_tag="ACHY09_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFS3888368.1"
                     /translation="VGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQTVTEK
                     ADAALDKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAEEHKLLI
                     SENSKQISTNTLG"
BASE COUNT          130 a           66 c           80 g           69 t
ORIGIN      
        1 gtaggtgttg gtgcaacgac aacaggacga catgctgtta caagacagat aactagcgtt
       61 gcagcaggtt ctgtcgatac ggatgctgtt aacgttgcac agctcaggga tttgcagact
      121 gtgacagaaa aggcagatgc agcgcttgat aaacgcataa caaacaacac cagtgcgata
      181 actaaaaatc tgcaggtggt taacagtaat aaagaggaaa tcagtaacat taataaaacg
      241 acaaaaattc tggatgaaag agtgacagcc aatacacaga cagcagaaga acataagctg
      301 cttatttcgg agaacagcaa acaaatcagt accaacactc ttggt
//
LOCUS       DAZUCC010000721          345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZUCC010000721 DAZUCC010000000
VERSION     DAZUCC010000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.726"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            157..>345
                     /locus_tag="ACHY09_005598"
     CDS             157..>345
                     /locus_tag="ACHY09_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS3888369.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAG"
BASE COUNT          103 a           71 c           93 g           78 t
ORIGIN      
        1 tagcgctatc atttctctgc cgccatgctt tggctatatt cggttcctgc atatacaatg
       61 caacaggata tttgctaccg tgggcaaaaa ctggaaagag taatggtaaa ataaaagtaa
      121 tgaaaacagc tggttgtaat tatccatgga gtcgaaatgg aaaaaacacg taagcgccgt
      181 ggtaccggac gggtgacgct gcaggaggtc gctaactttg caggtgtcgg aaccatgacc
      241 gtttcccgcg cgctgagaac accggaacag gtttctgaca agctgaggga aaagatagaa
      301 caggccgtag aagagttggg ttatattccc aacagaactg ctggc
//
LOCUS       DAZUCC010000722          345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZUCC010000722 DAZUCC010000000
VERSION     DAZUCC010000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.727"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>345
                     /locus_tag="ACHY09_005599"
     CDS             <1..>345
                     /locus_tag="ACHY09_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3888370.1"
                     /translation="TRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQT
                     MQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMV
                     MRMLMTLQGASP"
BASE COUNT           99 a           67 c          107 g           72 t
ORIGIN      
        1 tgacgcgcaa aggaaaagtc tgccatctgc tgacgtcgat gacggacgcc atgcgcttcc
       61 ccggaggaga aatggcggat ctgtacagtc atcgctggga aatcgaactg ggatacaggg
      121 agataaaaca gacgatgcaa ctgagcaggc tgacgctgag aagtaaaaag ccggagcttg
      181 tggagcaaga gctgtggggt gtcttactgg cttataatct ggtgagatat cagatgatta
      241 aaatggcgga acatctgaaa ggttactggc cgaatcaact gagtttctca gaatcatgcg
      301 gaatggtgat gagaatgctg atgacattgc agggcgcttc accgg
//
LOCUS       DAZUCC010000723          344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZUCC010000723 DAZUCC010000000
VERSION     DAZUCC010000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.728"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>344)
                     /locus_tag="ACHY09_005600"
     CDS             complement(<1..>344)
                     /locus_tag="ACHY09_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888371.1"
                     /translation="QRAEEARTSSLSSSDRPRPLSVSAFPDTLPGTEYGPDRGIRLSA
                     VWLMHDPAYPESLPGAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRMVAHRY
                     AGRPEMRYRYDD"
BASE COUNT           60 a          108 c          117 g           59 t
ORIGIN      
        1 catcgtcgta gcggtagcgc atctccggcc ttcccgcgta acggtgcgcc accatccggc
       61 ccgggtgctg cgcgtcatac gtgaaagcgc gcacctgcgt attgctgcgg tcatataccg
      121 ccagcagttc accggcttcc gtatacgtgt accgcaccag tggcgcaccg ggcaggctct
      181 ccgggtatgc cgggtcgtgc atcagccaca ccgccgaaag gcggataccc ctgtcggggc
      241 cgtattcggt accgggcagt gtgtcgggga acgctgagac tgagagaggg cggggacggt
      301 cagaagaaga tagcgaagag gtgcgggcct cttccgcacg ctgc
//
LOCUS       DAZUCC010000724          342 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZUCC010000724 DAZUCC010000000
VERSION     DAZUCC010000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.729"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>342
                     /locus_tag="ACHY09_005601"
     CDS             <1..>342
                     /locus_tag="ACHY09_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3888372.1"
                     /translation="LREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIA
                     ENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWP
                     VNRVSELLPWR"
BASE COUNT           77 a           88 c          107 g           70 t
ORIGIN      
        1 ggttgcgtga aaagatgaag accctgtcgc gacactcaga gttggcgaag gcgttcgcgt
       61 acgcacttaa ccagtggccg gcactgacgt actatgcgaa cgatggctgg gtggaaatcg
      121 acaacaacat cgctgaaaat gccctgcggg cggtcagtct gggtcgtaaa aacttcctgt
      181 tcttcggctc tgaccatggt ggtgagcggg gagcgctact gtacagcctg atcgggacgt
      241 gcaaactgaa tgacgtggat ccagaaagct accttcgcca tgtgcttggc gtcatagcag
      301 actggccggt caaccgggtc agcgaactgc ttccgtggcg ca
//
LOCUS       DAZUCC010000725          339 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZUCC010000725 DAZUCC010000000
VERSION     DAZUCC010000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.730"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>339
                     /locus_tag="ACHY09_005602"
     CDS             <1..>339
                     /locus_tag="ACHY09_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFS3888373.1"
                     /translation="KAALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYG
                     VSEKAAQIAAATGRLDSVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYR
                     LQFRVARKTL"
BASE COUNT           88 a           77 c          104 g           70 t
ORIGIN      
        1 aaaaagcggc gctgaaagcg caggagcttg cccggtatcc ccgggtggat ttaacgggat
       61 cagtgacccg ggatgacagg cagataggtg tcagtctgtc ctgggacctg ttcaaccgta
      121 atgccagtta tggtgtcagt gaaaaggccg cgcagatagc tgcagctacc ggacgactgg
      181 actctgttgc ccgaatgatt gatgaaaccg gacgattatc gctgataacg gtcagacaaa
      241 gtcggatgga aatggaaacg ctcagacgtc aggagcaggc ctctgccaga gttgtggact
      301 tttatcgtct tcagttccgg gtggcaagaa aaacactga
//
LOCUS       DAZUCC010000726          338 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.731, whole genome shotgun sequence.
ACCESSION   DAZUCC010000726 DAZUCC010000000
VERSION     DAZUCC010000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.731"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..>338)
                     /locus_tag="ACHY09_005603"
     CDS             complement(37..>338)
                     /locus_tag="ACHY09_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS3888374.1"
                     /translation="RQRDVAALDAKYSRELADARAENETLRADIAAGRKRLRINATCP
                     GTVREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAKEYIRTQCIN"
BASE COUNT           70 a           95 c           85 g           88 t
ORIGIN      
        1 gtaatttctt catgcattct ccggataaca aaaatactag ttaatacact gagtgcggat
       61 atattccttc gccccttcca gctgcttctg cattgtcatc aaccgttctc tgaggatgaa
      121 ataatcccgt tcagcggtgt ctgccagtcg ggggccggtt gcattatcca caccggaggt
      181 gccggtggct tcacgcacgg taccggggca ggtggcgttg atccgcaggc gcttacgacc
      241 agcggcaata tcagcacgca gagtttcatt ttcagctctc gcatcggcta attccctcga
      301 gtattttgca tcgagcgcag cgacatcacg ctggcgct
//
LOCUS       DAZUCC010000727          338 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZUCC010000727 DAZUCC010000000
VERSION     DAZUCC010000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.732"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>338)
                     /locus_tag="ACHY09_005604"
     CDS             complement(<1..>338)
                     /locus_tag="ACHY09_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFS3888375.1"
                     /translation="MKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESPVTPFV
                     PYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQTDDDK
                     AAAVVAQRVA"
BASE COUNT           75 a          103 c           84 g           76 t
ORIGIN      
        1 tcagccaccc gctgggccac caccgcagca gctttatcat catctgtctg ggtcacgaac
       61 ccggagcggg gatacacatt gccccacata ttctcttctt cctggctgcc tatttcgcgt
      121 ttaccgggga tcagggcctc cgggtacagt gactccggta gtcctgtacg ccaggcaata
      181 ctgtcaagcg tactgagaaa ataaggaaca aaaggtgtca ccggtgactc acaggaatat
      241 cccggcacca tccctccgat aagcgtggtg gccgggtgcc ctgttgcatc ggcatattta
      301 aaacggattg acgaacgacg ttccccctgt accttcat
//
LOCUS       DAZUCC010000728          337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZUCC010000728 DAZUCC010000000
VERSION     DAZUCC010000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.733"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..308
                     /locus_tag="ACHY09_005605"
     CDS             87..308
                     /locus_tag="ACHY09_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888376.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT           81 a           79 c           93 g           84 t
ORIGIN      
        1 ataacctcca gtgataaaat ggcattcagc tcgttaataa gagagttaac ttattaagcg
       61 ttagcgttta ttactgaggt aacaccatga aaagattaat tcctgtggca ttgctcaccg
      121 cattgctggc gggctgtgcg cacgattcgc cctgcgttcc ggtttatgac gatcagggtc
      181 gtctggttca caccaatacc tgtatgaaag gcacgactca ggataactgg gaaacggctg
      241 gggctattgc cggcggggca gcagctgttg ctggcctgac gatggggatc atcgctctgt
      301 cgaaataact aaacagggcg cggagctccg cgctctt
//
LOCUS       DAZUCC010000729          336 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.734, whole genome shotgun sequence.
ACCESSION   DAZUCC010000729 DAZUCC010000000
VERSION     DAZUCC010000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.734"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>336
                     /locus_tag="ACHY09_005606"
     CDS             <1..>336
                     /locus_tag="ACHY09_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888377.1"
                     /translation="KQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGY
                     CSAQGEQWEYRYDASGRRTEKRCDRKKIRFTYLWDGDNIAEIREYRDDKLYSVRHLVF
                     NGFELISQQ"
BASE COUNT           88 a           89 c          109 g           50 t
ORIGIN      
        1 gaaacaggcc ggaaacacgc agtatgacta tgacgccgca ggccggatgg tcagccgcac
       61 cagacaccgt gacggctacc gcccggaaac agagcggttc cggtgggaca gccgtgacca
      121 gctgaccggg tattgcagcg cacagggtga gcagtgggaa taccgctacg acgccagcgg
      181 caggcggacg gaaaaacgct gcgaccggaa gaaaatccgt tttacgtacc tgtgggacgg
      241 cgacaatatt gcggaaatcc gggaataccg cgatgataaa ctgtacagcg tacggcacct
      301 ggtgtttaac ggctttgagc tgataagcca gcagtg
//
LOCUS       DAZUCC010000730          333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.735, whole genome shotgun sequence.
ACCESSION   DAZUCC010000730 DAZUCC010000000
VERSION     DAZUCC010000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.735"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..63
                     /locus_tag="ACHY09_005607"
     CDS             <1..63
                     /locus_tag="ACHY09_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888378.1"
                     /translation="RKAAMDRNYLASVLAGSGLS"
     gene            73..333
                     /locus_tag="ACHY09_005608"
                     /pseudo
     CDS             73..333
                     /locus_tag="ACHY09_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           83 a           72 c           93 g           85 t
ORIGIN      
        1 cgaaaagcag ccatggacag aaactacctg gcgtcagtcc ttgctgggag cgggctttcg
       61 taatctttcc ctgggcggta tatcatccag gactcgattg ggctgaaggg gggatggaat
      121 ctgtaccaat atccgctgaa tccagtgcaa cacgttgatc cattagggct tagtactatg
      181 ataataggta acggccccgt tcctgataat ccttttgggc atgctgcggc ggcaaatcga
      241 tatggattaa tgagttcggg aactggggat gagatgggag cctctgtctc tgattatttt
      301 aaaaagatgc aaccccgcag agatacctgg att
//
LOCUS       DAZUCC010000731          330 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.736, whole genome shotgun sequence.
ACCESSION   DAZUCC010000731 DAZUCC010000000
VERSION     DAZUCC010000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.736"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..193
                     /locus_tag="ACHY09_005609"
     tRNA            99..193
                     /locus_tag="ACHY09_005609"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:133..135,aa:Sec,seq:tca)
BASE COUNT           72 a           88 c           84 g           86 t
ORIGIN      
        1 acgctgcgtg tattaggcgg aaaaaactga tctgggggat gtagaaactc aaggaagtag
       61 ctataatgcg ccccgcctcc atgtagcaat cgaggcgcgg aagatcgtcg tctccggtga
      121 ggcggctgga cttcaaatcc agttggggcc gccagcggtc ccgggcaggt tcgactcctg
      181 tgatcttccg ccaattaaca tcttctgaac tctcccgatg tcaatccccc ccttctaatc
      241 ccttgtatat caggattcct ttgtatccag aagtcaaccg acttcacccc gtatcaagtt
      301 gcttttgggg gtactttagg gggtattatt
//
LOCUS       DAZUCC010000732          326 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.737, whole genome shotgun sequence.
ACCESSION   DAZUCC010000732 DAZUCC010000000
VERSION     DAZUCC010000732.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.737"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..257)
                     /locus_tag="ACHY09_005610"
     CDS             complement(<1..257)
                     /locus_tag="ACHY09_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFS3888379.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWI"
BASE COUNT           95 a           67 c           70 g           94 t
ORIGIN      
        1 tcgatccagt ttttattgat cgacaaatcc atcaatttat ggacgttttc gtcgtcccag
       61 gtctggcaga aagtggccag gttctgacga gcgttgccat caagatataa ttcatcattg
      121 ataatctgaa atgcaacatc atcgcgcatt tcgtgcagcg gaaatcgttt tgactccgcg
      181 atagtagaaa tggcctttgc accaaaacgt gaatcgagta gttctgagcg gaaatccgtt
      241 aacagcttct ggtccatttc gaactcctta aatttatttg aaggcaataa aaaagtagga
      301 tttatccgca atggaagcaa ggcatt
//
LOCUS       DAZUCC010000733          324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.738, whole genome shotgun sequence.
ACCESSION   DAZUCC010000733 DAZUCC010000000
VERSION     DAZUCC010000733.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.738"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            27..>324
                     /locus_tag="ACHY09_005611"
     CDS             27..>324
                     /locus_tag="ACHY09_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS3888380.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLENMDAGELFLWQVGSAAM
                     LSWFVLAISRKKLPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITAVIFSL"
BASE COUNT           95 a           53 c           65 g          111 t
ORIGIN      
        1 tctttatact gaggagataa ctaaatatga ttaagggaat tatgtttgca tttcttgctg
       61 ctttttcatg gggagcagca atagtgatga gcaaaaaagg tctcgaaaac atggatgccg
      121 gagagttgtt tttatggcaa gttggtagtg ctgcaatgtt atcatggttt gttctcgcaa
      181 ttagcagaaa aaaactcccg gtaacaaaaa aatcaatcct tgcctattca acaggaattt
      241 ttgagccatt tctagcgtat acattcactc tctatggcct aaaatttatt tctgctggta
      301 taacagctgt tattttttca cttg
//
LOCUS       DAZUCC010000734          322 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.739, whole genome shotgun sequence.
ACCESSION   DAZUCC010000734 DAZUCC010000000
VERSION     DAZUCC010000734.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.739"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            159..322
                     /locus_tag="ACHY09_005612"
                     /pseudo
     CDS             159..322
                     /locus_tag="ACHY09_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT           98 a           64 c           78 g           82 t
ORIGIN      
        1 tttcaccatg ttatagtttt atttgtgaat taaatcaatt atggcaatga attacaaggg
       61 gttaaatgct gccgcagcat agcgatattg aaatagcctg gtatgcttca atacagcagg
      121 agccgaatag ctggaagacc gtcaccacac agttctacat ccaggaattc agtgagtata
      181 ttgcgccact gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa agatcgttgc
      241 tggaagcctg aaaaaagtat cgggtgctgc taaaccgtgt ggacacttcc gtagcaccag
      301 atatcgagtg gcttattcaa cc
//
LOCUS       DAZUCC010000735          317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.740, whole genome shotgun sequence.
ACCESSION   DAZUCC010000735 DAZUCC010000000
VERSION     DAZUCC010000735.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.740"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>317
                     /locus_tag="ACHY09_005613"
     CDS             <1..>317
                     /locus_tag="ACHY09_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFS3888381.1"
                     /translation="EGIVITTVRSRGSRKSRFTLTFEGIPYSETVCWRPALLSRPVIS
                     GSLPARIESHEKNDIYAHLDDQGRYRVKLDFDRNSTERGYAYLWLRLAKPCAGDTYGF
                     HSPL"
BASE COUNT           62 a           88 c           94 g           73 t
ORIGIN      
        1 gaaggcatcg ttatcaccac ggtgcgttcc cgcggttcac gcaaaagtcg ctttacgctg
       61 acgtttgagg ggatccccta cagtgaaacg gtctgctggc gtccggcact gctcagtcgc
      121 ccggtgatat ccggctcgct tccggcgcgg attgagagtc acgagaagaa cgatatctac
      181 gctcatctgg acgatcaggg gcgttaccgg gtgaagctgg actttgaccg caacagtaca
      241 gaacggggtt acgcttatct gtggttgcgt ctggctaaac cctgtgccgg tgatacgtac
      301 ggtttccact caccgct
//
LOCUS       DAZUCC010000736          317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.741, whole genome shotgun sequence.
ACCESSION   DAZUCC010000736 DAZUCC010000000
VERSION     DAZUCC010000736.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.741"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>317
                     /locus_tag="ACHY09_005614"
     CDS             <1..>317
                     /locus_tag="ACHY09_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFS3888382.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVY"
BASE COUNT           80 a           71 c           90 g           76 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacat
//
LOCUS       DAZUCC010000737          314 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.742, whole genome shotgun sequence.
ACCESSION   DAZUCC010000737 DAZUCC010000000
VERSION     DAZUCC010000737.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.742"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>314
                     /locus_tag="ACHY09_005615"
     CDS             <1..>314
                     /locus_tag="ACHY09_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS3888383.1"
                     /translation="PVLPDVREFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTA
                     LARQTLAELTGIINAGRYSKVLPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSA
                     RC"
BASE COUNT           72 a           88 c           85 g           69 t
ORIGIN      
        1 ccggttctgc cggatgtgcg tgagttcatc cgcggcacgg tcaatgtcat ggaaaacctt
       61 atgccctacc gtgaactggg tattcccctg ccgaaagtcc gcaccctggt taactgcatg
      121 gattatacgg cgctggccag acagacactg gcagagctga caggcattat caacgccggt
      181 cgctacagca aagtgctgcc tgaaggtgcg gtcacccttc tttcaacaca gatttacgac
      241 ctgaatattt ataaacaggg gcacgcagcc ggtcagccgg tgcatcgtct ggaaaaagtg
      301 tctgcccgtt gcag
//
LOCUS       DAZUCC010000738          313 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.743, whole genome shotgun sequence.
ACCESSION   DAZUCC010000738 DAZUCC010000000
VERSION     DAZUCC010000738.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.743"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..>313
                     /locus_tag="ACHY09_005616"
     CDS             54..>313
                     /locus_tag="ACHY09_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFS3888384.1"
                     /translation="MKPFSVALGAGSVADRGAGIEAYMPETKRWNPGDVVKSRMSDKQ
                     LNRLMEIDKLISAYNSQETLSAEETKQLEEVKKERADMFATY"
BASE COUNT           95 a           67 c           80 g           71 t
ORIGIN      
        1 catccccgtc aatacggccc tcgctgtacg cttacggtgc aaacgcaaca acattgaaac
       61 cattctctgt cgctctgggc gcaggaagtg ttgctgatcg tggcgctggt attgaagcat
      121 atatgccaga gacaaagcgc tggaatccag gggatgtagt aaaatccagg atgtctgaca
      181 agcagcttaa caggcttatg gaaatcgata agctaatatc cgcttataat tcacaagaga
      241 cgctgtctgc tgaagagact aaacaactgg aggaagtgaa aaaagagcgg gctgatatgt
      301 ttgcgacata tat
//
LOCUS       DAZUCC010000739          312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.744, whole genome shotgun sequence.
ACCESSION   DAZUCC010000739 DAZUCC010000000
VERSION     DAZUCC010000739.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.744"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>312
                     /locus_tag="ACHY09_005617"
     CDS             <1..>312
                     /locus_tag="ACHY09_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888385.1"
                     /translation="KDGAINPDEHTELTAKNQAVTDAKNPAEAAVNALPEGDVKAELK
                     GRLDAVDGISVPEVNDQNSNGKPDAQDAKDAKDAEDAAKALVEAAEAKQAEAEGALTE
                     AN"
BASE COUNT           90 a           69 c          117 g           36 t
ORIGIN      
        1 aaagacggag ccattaatcc ggatgagcat accgaactga cggcgaagaa ccaggcggtg
       61 acggatgcga agaacccggc tgaagcagcg gtgaatgccc tgccggaagg tgacgtgaag
      121 gctgagctga aaggccgact ggatgcggtt gacgggatca gcgtaccgga agtgaacgat
      181 cagaacagca acggcaagcc ggatgcacag gatgcgaagg atgcgaagga tgcagaagat
      241 gccgcgaagg cgctggttga ggcagccgaa gcgaagcagg ctgaagcgga aggtgcgctg
      301 acggaggcca at
//
LOCUS       DAZUCC010000740          312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.745, whole genome shotgun sequence.
ACCESSION   DAZUCC010000740 DAZUCC010000000
VERSION     DAZUCC010000740.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.745"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>312
                     /locus_tag="ACHY09_005618"
     CDS             <1..>312
                     /locus_tag="ACHY09_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFS3888386.1"
                     /translation="LSSRTLMNEIFDAEIIDDNENFKDSDAYSRLKAKYPAAFPAGVK
                     PSKIKKATDGKKRTKMRIGKFSELKELWDLINQKVVIEYKINSENEFLSIFKSFMIEE
                     T"
BASE COUNT          123 a           47 c           59 g           83 t
ORIGIN      
        1 agctttcatc cagaacactc atgaatgaga tctttgatgc tgaaattatt gatgataatg
       61 aaaacttcaa agattccgat gcctatagcc gtttaaaagc aaaatatccg gctgcattcc
      121 ctgccggggt gaaaccaagc aaaataaaaa aagccacgga tggaaaaaag cgtactaaaa
      181 tgcgtattgg taagttcagt gagttgaaag aactatggga tttgatcaat caaaaagtag
      241 taattgagta taaaataaat agtgaaaatg aattcttatc cattttcaaa tcattcatga
      301 ttgaagaaac ag
//
LOCUS       DAZUCC010000741          307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.747, whole genome shotgun sequence.
ACCESSION   DAZUCC010000741 DAZUCC010000000
VERSION     DAZUCC010000741.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.747"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>307
                     /locus_tag="ACHY09_005619"
     CDS             <1..>307
                     /locus_tag="ACHY09_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888387.1"
                     /translation="VTSKTDGVADAPALTIPEVADSVANAAELKDGLQAEVKLPAGTV
                     EGAVITLTVTHPDKTTRTETHTVSKDEAADGTVSMVVPKGSVVDGQNSVSVSLTQGS"
BASE COUNT           83 a           63 c           95 g           66 t
ORIGIN      
        1 ctgtgacgtc aaaaactgat ggtgtggcag atgctccggc cctgaccatt ccagaagtgg
       61 ctgatagtgt ggccaatgcg gctgagctga aagatggttt acaggcagaa gtcaagttgc
      121 cggcaggaac agtggaaggt gctgtcatta cactgacagt gactcatccg gataaaacca
      181 ccagaaccga gacgcatact gttagcaaag atgaggctgc agacggtact gtgtcaatgg
      241 ttgttccgaa gggaagtgtt gttgacggtc agaacagtgt gagtgtcagc ctgactcagg
      301 gcagtaa
//
LOCUS       DAZUCC010000742          307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.748, whole genome shotgun sequence.
ACCESSION   DAZUCC010000742 DAZUCC010000000
VERSION     DAZUCC010000742.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.748"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>307
                     /locus_tag="ACHY09_005620"
     CDS             <1..>307
                     /locus_tag="ACHY09_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFS3888388.1"
                     /translation="TGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAYAM
                     RTDLKNADKLVVNQKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQA
                     "
BASE COUNT          101 a           59 c           80 g           67 t
ORIGIN      
        1 tgacaggaga ctcctcactt cgctcgttaa aaaataccgg aagcatgaca tattttacag
       61 gaagtgcagc gaataaagca ttccatacac tgacggttga tgagctgacg acgaatggca
      121 ctgcgtatgc catgcgtacg gacctgaaaa atgcggataa gctggtagta aaccaaaagc
      181 tgtcaggtaa ggacaatatt ctgctggttg attttctgaa caaacccacc ggagaaaaac
      241 tggatattga actggtgagt gcaccgggga acagcagtaa ggatgttttt aaaggaagtg
      301 aacaggc
//
LOCUS       DAZUCC010000743          304 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.749, whole genome shotgun sequence.
ACCESSION   DAZUCC010000743 DAZUCC010000000
VERSION     DAZUCC010000743.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.749"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..304)
                     /locus_tag="ACHY09_005621"
                     /pseudo
     CDS             complement(1..304)
                     /locus_tag="ACHY09_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           72 a           79 c           78 g           75 t
ORIGIN      
        1 agaatgccgg gagcctgcag gggcgcttcg tgaatttcac ggatacattc tgcgtcatga
       61 aaattttctc tgtaccgggt tcattccggc agtatgtggt gcatatggag cattctgttc
      121 cgtgcggcgc tgagctcatc aatatagcgc ccccggaaaa cgggaccgtc atgcatatag
      181 cacagaggga acacttccac attgtcacaa tattcccgcc cccactcttt tgtgacggag
      241 gcgtcatcgg tgtaaaacca tgtgtcgggc atgccgttag ccagaaagca aatgttccat
      301 acat
//
LOCUS       DAZUCC010000744          296 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.750, whole genome shotgun sequence.
ACCESSION   DAZUCC010000744 DAZUCC010000000
VERSION     DAZUCC010000744.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.750"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>296
                     /locus_tag="ACHY09_005622"
     CDS             <1..>296
                     /locus_tag="ACHY09_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888389.1"
                     /translation="SEQPLVLTNVAAGNVSPVSTDAVNGSQLASAARIAGGDNVVWKD
                     GKAALAPDTFSELTTAGGGKTTVNGGVHKTPDNVADAVNMLNREGTKYFKAASSG"
BASE COUNT           76 a           63 c           92 g           65 t
ORIGIN      
        1 agtgagcagc ctcttgttct gacaaatgtg gcggccggaa atgtgtcacc ggtatccacg
       61 gatgcggtta acgggagcca gctggcatct gcagccagaa ttgctggcgg tgataatgtt
      121 gtctggaaag acggaaaagc agcgctggct cctgacacct tcagtgagct gacaactgca
      181 ggtggcggta agacaacggt gaatggtggt gtacataaaa caccggataa tgtggctgat
      241 gcagtgaata tgctgaaccg tgaaggcacg aagtatttta aagctgcctc ttccgg
//
LOCUS       DAZUCC010000745          296 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.751, whole genome shotgun sequence.
ACCESSION   DAZUCC010000745 DAZUCC010000000
VERSION     DAZUCC010000745.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.751"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..273
                     /locus_tag="ACHY09_005623"
     CDS             <1..273
                     /locus_tag="ACHY09_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFS3888390.1"
                     /translation="WLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGTMFLVL
                     PAVWLGALSWAGVHIGDGISRGLPNGISEAKGAASSAGSIANRGIK"
BASE COUNT           78 a           49 c           93 g           76 t
ORIGIN      
        1 tggcttgaca gctggatgct gacggcgctg tacagttcgg atacacatac ccggttcaat
       61 atgatgggat tccagaacac atcggatgat ctgataatga atctggtgat ggggacgatg
      121 tttctcgtct tgccggcagt gtggctggga gcgttgtcct gggctggtgt tcatattggc
      181 gatggaatca gtcgaggatt accgaatggt atttcggaag cgaaaggtgc agcttcgagt
      241 gcaggaagta ttgctaatcg aggtataaaa taggaagtaa aaaagttaca ggagaa
//
LOCUS       DAZUCC010000746          294 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.752, whole genome shotgun sequence.
ACCESSION   DAZUCC010000746 DAZUCC010000000
VERSION     DAZUCC010000746.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.752"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(7..>294)
                     /locus_tag="ACHY09_005624"
     CDS             complement(7..>294)
                     /locus_tag="ACHY09_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888391.1"
                     /translation="MSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFP
                     DESASLVLYFSNNDIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
BASE COUNT           96 a           49 c           65 g           84 t
ORIGIN      
        1 taaaaattaa agtatttgat tggcatcggt taactgatca ataaccagtg ccagattggt
       61 aaacattttg aaaaagcgat actggctcgg aatatctctt tcaaagacga aacattcaat
      121 gtcattgttt gaaaaataaa ggactagtga tgctgattcg tcaggaaaaa tatggaaata
      181 tatctggtta ccagaaggag gatagtccag aagaagggta tctttgcccg tcacatacct
      241 gttgtggtcg tggatgctgc gctcacactt catcagaggt gtcatgatac tcat
//
LOCUS       DAZUCC010000747          294 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.753, whole genome shotgun sequence.
ACCESSION   DAZUCC010000747 DAZUCC010000000
VERSION     DAZUCC010000747.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.753"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           89 a           57 c           51 g           97 t
ORIGIN      
        1 tttattatgt tatgttttat ggtacgcttt aaaaagcgta atcataccct tacttgtaca
       61 tcaccggcgc ttcttacagt tgtaagaata acatcacaaa tgtcgcggcc ctctctgatt
      121 accgcgaacc aatatttatc atattcttct tttcgtcctc actacttacc atgcagaaag
      181 gaattggaag tggcgatgaa atgattatca gcacttgata caggaattta aaaatatcga
      241 gtgcagctac gacgatattt tgcggattat ttatagagag cattatgcct taaa
//
LOCUS       DAZUCC010000748          291 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.754, whole genome shotgun sequence.
ACCESSION   DAZUCC010000748 DAZUCC010000000
VERSION     DAZUCC010000748.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.754"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           74 a           77 c           60 g           80 t
ORIGIN      
        1 gcaccttacc cttacactgc ctgtcgacac tggtttacgt ttgttgagtc gccatgttgc
       61 actccggatc agagcactac ctgcggcacc acccgtccgg tagccaggac ttgaaatggg
      121 ttcgatccgg cgcaacttcc caacagtcag gattataaat ctcattatct taattgcaac
      181 acggtccagc ggtggttata attcgttcgt tgagatgcca tgttgcacta tcagtaactt
      241 aacatcaaag tagtaaataa aagaacctgc ctcaccagca ggttctttta t
//
LOCUS       DAZUCC010000749          291 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.755, whole genome shotgun sequence.
ACCESSION   DAZUCC010000749 DAZUCC010000000
VERSION     DAZUCC010000749.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.755"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>291)
                     /locus_tag="ACHY09_005625"
     CDS             complement(<1..>291)
                     /locus_tag="ACHY09_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888392.1"
                     /translation="ENKTSFGSMGIQIRTAAITLGLDPSELTTRNQLELATCLMEDDF
                     TFKIAATHLRDLALFDYPSSATLYMTNEQYIMAGIRYNRGVERDLGFFIYLI"
BASE COUNT           99 a           55 c           49 g           88 t
ORIGIN      
        1 ttaattaaat aaataaaaaa acccaaatct cgttcaacac cacgattata tctaatacca
       61 gccataatat actgctcatt cgtcatatac agtgttgccg aagagggata atcaaaaagg
      121 gcaagatctc ttaaatgagt agcagcaatt ttaaaggtaa aatcatcttc catcaggcat
      181 gtcgctaact ctaactgatt tctggtagtt aattctgaag ggtcaaggcc tagagtaatc
      241 gctgcggttc gtatttgtat acccattgag ccaaatgatg ttttattttc a
//
LOCUS       DAZUCC010000750          287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.756, whole genome shotgun sequence.
ACCESSION   DAZUCC010000750 DAZUCC010000000
VERSION     DAZUCC010000750.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.756"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>287)
                     /locus_tag="ACHY09_005626"
     CDS             complement(<1..>287)
                     /locus_tag="ACHY09_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888393.1"
                     /translation="ANVGIPADKVTQDGEYTVKAEITDPAGNTSGQGKATQFGVDTVA
                     PSEPALKAENDGSVSATLPDGANKGDKVEVTFTDEEGNEQKVTLEKGDGNW"
BASE COUNT           52 a           95 c           55 g           85 t
ORIGIN      
        1 ctccagttgc catccccttt ctccagcgtg actttctgct cattaccctc ttcatccgta
       61 aaggtcactt ccaccttgtc acctttgttc gcaccatctg gcagagtcgc tgatacagaa
      121 ccatcattct cagccttcag tgctggttct gacggagcta cagtatccac gccaaactgg
      181 gtagctttac cctgaccact ggtgttgcct gccgggtctg tgatttcagc cttaactgtg
      241 tattccccat cctgcgtcac tttatccgca ggaatgccca cattcgc
//
LOCUS       DAZUCC010000751          287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.757, whole genome shotgun sequence.
ACCESSION   DAZUCC010000751 DAZUCC010000000
VERSION     DAZUCC010000751.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.757"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..>287
                     /locus_tag="ACHY09_005627"
     CDS             11..>287
                     /locus_tag="ACHY09_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFS3888394.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYY"
BASE COUNT           66 a           65 c           94 g           62 t
ORIGIN      
        1 gatgagaatg atgagtgata tgtcgcatca ggaacgtctg atcaggcggg tcaggaaact
       61 gctggagctg agccgtaata acagcaatgc ccatgaggcc gggctggcgc ttgcaagggc
      121 gcaacagctg atggcgaaac acggtatcac ggagcaggat gccggacttt cttcggtgca
      181 gacctcttca tcacaggggg caccttctga tgctgaaaaa gttccggcgt ggatgtttcg
      241 tctggtcatg gtggtgacac agacatttgg ctgccgggcg tattacg
//
LOCUS       DAZUCC010000752          287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.758, whole genome shotgun sequence.
ACCESSION   DAZUCC010000752 DAZUCC010000000
VERSION     DAZUCC010000752.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.758"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(143..>287)
                     /locus_tag="ACHY09_005628"
     CDS             complement(143..>287)
                     /locus_tag="ACHY09_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFS3888395.1"
                     /translation="LFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGS
                     GLS"
BASE COUNT           81 a           63 c           57 g           86 t
ORIGIN      
        1 ttacctcttg aaattcaaat gggaatccag tgaaaggacc tttaggcata atatatcttt
       61 gatatccata aagctcaccg agcacttgta ggcttatagg cgcatttgta ttcatagagg
      121 ggttgttaaa cccagggaaa gattacgaaa gcccgctccc tgcaaggact gacgccaggt
      181 agtttctgtc catggctgct tttcgcatct tacgtcttaa ccctgccttg aataccttat
      241 cattcgttaa aatattaata gcgatgtgcc gtatccctga aaataat
//
LOCUS       DAZUCC010000753          286 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.759, whole genome shotgun sequence.
ACCESSION   DAZUCC010000753 DAZUCC010000000
VERSION     DAZUCC010000753.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.759"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>286)
                     /gene="tnpB"
                     /locus_tag="ACHY09_005629"
     CDS             complement(<1..>286)
                     /gene="tnpB"
                     /locus_tag="ACHY09_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS3888396.1"
                     /translation="TKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVFIFRGRSG
                     SQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW"
BASE COUNT           61 a           91 c           77 g           57 t
ORIGIN      
        1 ccagtcgata ccttccagca gcatcgccag ctgcgcctgc gtaaggaaca ctttgccatc
       61 acgggctgac ggccaggcga agcgcccacg ctccagccgt ttggtcagga ggcacagtcc
      121 gtcaccggtg gaccacagca gtttaacctg actgccgctg cggccccgga aaatgaaaac
      181 atggccggac atgggatcgt ctttcagcgc cgtttgtact ttcgcagcca ggccgttgaa
      241 gccatttctc atatcggtga taccggcaac cagccaaatt ttggtc
//
LOCUS       DAZUCC010000754          286 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.760, whole genome shotgun sequence.
ACCESSION   DAZUCC010000754 DAZUCC010000000
VERSION     DAZUCC010000754.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.760"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           74 a           83 c           58 g           71 t
ORIGIN      
        1 ttgcctggtt tgattattgt cgcagttatt ttatatcgct ttcccatact ggctctcatc
       61 tcaacaacaa aatcaccggc tgagccatca ctccggtaat gcatgcgaaa aaaagtgagc
      121 gtctcagcaa tggctcatgc ggtgattctg tacggtaccc gcacaaaata cgggcaccaa
      181 cagcgtcgca gaatacaacg aaggcatcag ggcagcacct ctctcccgct atttttcaga
      241 gttctttacc ctcctcctga cacgggagta caaccctgac agcatc
//
LOCUS       DAZUCC010000755          284 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.761, whole genome shotgun sequence.
ACCESSION   DAZUCC010000755 DAZUCC010000000
VERSION     DAZUCC010000755.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.761"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>284)
                     /locus_tag="ACHY09_005630"
     CDS             complement(<1..>284)
                     /locus_tag="ACHY09_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HFS3888397.1"
                     /translation="LGQKCRVVLVGDCYQQIYRFRGANNALSHPALKNADRLWLTQSF
                     RFGPAVARMANLLLQREGETREVKGCGGDDEVLLKCHAREHLQGHYTVLS"
BASE COUNT           76 a           93 c           56 g           59 t
ORIGIN      
        1 gactgagtac cgtgtaatgc ccctgcagat gttcacgcgc atggcatttc agcaatacct
       61 catcatcacc accacaacct ttcacctctc gtgtttcacc ttcacgctgc agaagcagat
      121 ttgccatacg agccaccgcc ggaccaaaac ggaaactctg tgtcagccac aaccggtccg
      181 cattcttcag tgcaggatgg gaaagcgcat tattcgcacc ccgaaagcgg taaatctgtt
      241 gataacaatc tccaacaagg accacccgac acttctgccc caga
//
LOCUS       DAZUCC010000756          283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.762, whole genome shotgun sequence.
ACCESSION   DAZUCC010000756 DAZUCC010000000
VERSION     DAZUCC010000756.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.762"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>283)
                     /locus_tag="ACHY09_005631"
     CDS             complement(<1..>283)
                     /locus_tag="ACHY09_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888398.1"
                     /translation="INKLLLDVDNPRFPTSAENQRDAIAKMLDLQYDRIYRLAKDIAS
                     KGLDPSENILVYPSEEESGFFVVAEGNRRVTALKLLHSPRLAPTEKARKA"
BASE COUNT           85 a           59 c           59 g           80 t
ORIGIN      
        1 aagctttccg tgctttttct gttggtgcaa gtctaggaga gtgtaaaagt ttaagggctg
       61 taactcgacg attaccttca gcaacgacga aaaacccaga ttcctcttca ctaggataaa
      121 ctaatatgtt ttcagacgga tctaaacctt tcgaagcaat atctttagcc aagcgataaa
      181 tgcggtcgta ctgcaagtca agcatcttgg caatagcatc acgctgattt tctgctgacg
      241 tcgggaaacg agggttatca acatccaaaa gtaacttatt gat
//
LOCUS       DAZUCC010000757          282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.763, whole genome shotgun sequence.
ACCESSION   DAZUCC010000757 DAZUCC010000000
VERSION     DAZUCC010000757.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.763"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>282)
                     /locus_tag="ACHY09_005632"
     CDS             complement(<1..>282)
                     /locus_tag="ACHY09_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFS3888399.1"
                     /translation="QGGEVYRGYGGNADMTTMNAVARIAGTAGSAVAALGAFPALDAM
                     RQALPMVQSIILMAIYIMIPLILAFAAYELSTLITLTFVVFAVHFLTFWW"
BASE COUNT           71 a           97 c           58 g           56 t
ORIGIN      
        1 ccaccagaac gtcagaaagt gtaccgcaaa caccacaaac gtcagtgtga ttaacgtcga
       61 cagttcatat gccgcaaacg ccagaatcag cggtatcatg atgtaaatcg ccatcagtat
      121 tatcgactgt accatcggca gtgcctgccg cattgcatcc agtgccggaa acgcacccag
      181 tgctgctacc gcactgcccg ccgttcccgc aatccgggcc accgcattca tggtcgtcat
      241 atctgcattg ccaccatagc cacgatacac ctctcccccc tg
//
LOCUS       DAZUCC010000758          282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.764, whole genome shotgun sequence.
ACCESSION   DAZUCC010000758 DAZUCC010000000
VERSION     DAZUCC010000758.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.764"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>282)
                     /locus_tag="ACHY09_005633"
     CDS             complement(<1..>282)
                     /locus_tag="ACHY09_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888400.1"
                     /translation="LYSRIYGVEWPSGNNVRHARDEMSSLVLLIDTPWYPPSGELVGE
                     ISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDLQAEWPGRETLPQPR"
BASE COUNT           58 a           92 c           68 g           64 t
ORIGIN      
        1 tcggggctgt ggaagtgttt ccctgcccgg ccattcggcc tgcagatcac tgtccgtatg
       61 ctctccgcca tcgtaacagt tgtaaccctg tataccgtgt accggctcgc tgtaccagtg
      121 tcggacctcg cagtcaaaca ccttcgagat ctcaccaacc agttcgcctg aaggcggata
      181 ccagggcgtg tcgatcagca gcaccagact gctcatctca tcacgcgcat ggcgaacatt
      241 atttcctgac ggccactcca caccgtatat ccggctgtaa ag
//
LOCUS       DAZUCC010000759          275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.765, whole genome shotgun sequence.
ACCESSION   DAZUCC010000759 DAZUCC010000000
VERSION     DAZUCC010000759.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.765"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            178..>275
                     /locus_tag="ACHY09_005634"
     CDS             178..>275
                     /locus_tag="ACHY09_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS3888401.1"
                     /translation="MSEDVPLPKANQRYRDDHGALVTVTSVEETRVV"
BASE COUNT          109 a           48 c           63 g           55 t
ORIGIN      
        1 tatcagaaaa agagaaggac atgaaagagg agagcaataa aagacacgga aaataaagaa
       61 gagagagaga aaatatttct taaaaacaga agattaacaa aacacatcag gaggcagaga
      121 ctcctgattc agcaatatca cacgaaattt tagttaactg ctaccgttct gaaatctatg
      181 tctgaagatg ttccattacc gaaagccaat cagcgataca gagacgatca tggtgcgctt
      241 gtcaccgtga ccagtgttga ggaaacccgg gtggt
//
LOCUS       DAZUCC010000760          271 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.766, whole genome shotgun sequence.
ACCESSION   DAZUCC010000760 DAZUCC010000000
VERSION     DAZUCC010000760.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.766"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>271
                     /locus_tag="ACHY09_005635"
     CDS             <1..>271
                     /locus_tag="ACHY09_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888402.1"
                     /translation="ITNGDKGLKISLNNDLTVGDKAPGSIKVNGADGKEAVSLNGQDG
                     TIKLTSSPAANGKSASATIAVNAGKGGLTDAAGTTKTRLTYAPDGT"
BASE COUNT           79 a           57 c           77 g           58 t
ORIGIN      
        1 atcaccaatg gggataaagg gttaaaaata tccctgaata atgacctgac tgtgggggat
       61 aaggctcccg gcagcatcaa agttaacggg gctgatggta aggaggctgt gagtctgaat
      121 ggtcaggatg gcacgattaa actgacatcc tcgcctgctg cgaacggaaa gtctgcgtca
      181 gcaaccattg ctgttaatgc aggcaaaggt ggattaacag atgctgccgg taccacgaag
      241 acacgtctga cttatgctcc agatggaaca t
//
LOCUS       DAZUCC010000761          270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.767, whole genome shotgun sequence.
ACCESSION   DAZUCC010000761 DAZUCC010000000
VERSION     DAZUCC010000761.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.767"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..165
                     /gene="ymgF"
                     /locus_tag="ACHY09_005636"
     CDS             <1..165
                     /gene="ymgF"
                     /locus_tag="ACHY09_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFS3888403.1"
                     /translation="AFMLITLLVILIAKPSTGLGEVLVTINLLNALVWLAINLINRLR
                     ERLVSHRDQH"
BASE COUNT           69 a           55 c           67 g           79 t
ORIGIN      
        1 gcatttatgc tgatcactct cctggtcatc cttattgcaa aacccagtac cgggctggga
       61 gaagtgcttg tgacgataaa tttgcttaat gcccttgttt ggctggcgat caatctgatt
      121 aatcgattaa gggaaagact cgtaagccac agggatcagc actaatcttt cagtttctca
      181 ctgtcagtat gcggctgaat gggttgctgg cagtgaacgc ctggatcatt gaagaaagga
      241 attattgcgc aaatagttgt caactctggt
//
LOCUS       DAZUCC010000762          261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.768, whole genome shotgun sequence.
ACCESSION   DAZUCC010000762 DAZUCC010000000
VERSION     DAZUCC010000762.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.768"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>261
                     /locus_tag="ACHY09_005637"
     CDS             <1..>261
                     /locus_tag="ACHY09_005637"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888404.1"
                     /translation="PVSVMAKAQKGSVINSITFTDSLTDETDDKHDFTNTGDLKGSTP
                     SIMTFPYGESDDRYTTNYVGNVQSSKTKFINLATGLTNDTTPT"
BASE COUNT           89 a           49 c           50 g           73 t
ORIGIN      
        1 cccagtcagt gtgatggcaa aagcacagaa aggtagcgtc attaattcca ttaccttcac
       61 tgatagttta actgatgaaa ccgatgataa acatgatttt actaatacag gtgatctgaa
      121 gggcagtacg ccttctatca tgacatttcc gtatggtgaa tcagatgata ggtatacgac
      181 taactatgtt ggtaatgttc agtcctcaaa aacaaaattt ataaatcttg caacagggtt
      241 aacgaatgat actacgccaa c
//
LOCUS       DAZUCC010000763          254 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.769, whole genome shotgun sequence.
ACCESSION   DAZUCC010000763 DAZUCC010000000
VERSION     DAZUCC010000763.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.769"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>254
                     /locus_tag="ACHY09_005638"
     CDS             <1..>254
                     /locus_tag="ACHY09_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3888405.1"
                     /translation="DNSDKDDKIEQEINEILHDKELLLSLENPRRFIFSKWTLREGWD
                     NPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNEYMCRVKNR"
BASE COUNT           77 a           46 c           59 g           72 t
ORIGIN      
        1 gataatagtg ataaagatga taaaattgag caggaaatca acgaaatcct tcatgataaa
       61 gagcttctat tgtctttaga aaatcctcgc cgtttcattt tctctaaatg gacgttgcgt
      121 gagggatggg ataacccaaa tgtctttcag atttgtaagc tccgttcgag cggtagcacc
      181 acatctaaac ttcaggaggt agggcgtggt ttacgtcttc cagtgaatga atacatgtgc
      241 cgtgtgaaaa accg
//
LOCUS       DAZUCC010000764          249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.770, whole genome shotgun sequence.
ACCESSION   DAZUCC010000764 DAZUCC010000000
VERSION     DAZUCC010000764.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.770"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..204
                     /locus_tag="ACHY09_005639"
     CDS             <1..204
                     /locus_tag="ACHY09_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFS3888406.1"
                     /translation="EGPFSRGQAVAVTTAYRNVFTEDDQGTYFRLVIRNAEGQLRWRC
                     WNFESDAGKQLNVWLSSEGLLRQ"
BASE COUNT           56 a           65 c           67 g           61 t
ORIGIN      
        1 gaaggccctt tctcacgcgg acaggctgtt gccgtcacaa cagcttaccg caatgtgttt
       61 actgaagatg accagggaac atatttccgg ctggttatcc gtaatgccga agggcagcta
      121 cgctggcggt gctggaattt tgagtctgac gccggaaaac agcttaatgt gtggctctcc
      181 agtgaagggc ttctcaggca ataaacgtct tcatttcatc catcaggccg catcttctcc
      241 gggagacgc
//
LOCUS       DAZUCC010000765          247 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.771, whole genome shotgun sequence.
ACCESSION   DAZUCC010000765 DAZUCC010000000
VERSION     DAZUCC010000765.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.771"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>247
                     /locus_tag="ACHY09_005640"
     CDS             <1..>247
                     /locus_tag="ACHY09_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3888407.1"
                     /translation="KNGTLSKKSRLGEAFSYVLNQWDALCYYSDDGLAEADNNAAERA
                     LRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGI"
BASE COUNT           59 a           52 c           78 g           58 t
ORIGIN      
        1 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
       61 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga taataatgct
      121 gcggaaagag cgcttcgtgc agtctgtctc ggaaagaaaa actttatgtt ctttggcagc
      181 gatcacggcg gcgagcgtgg agcactgttg tacgggctga tcggcacctg ccgtctgaac
      241 ggtatcg
//
LOCUS       DAZUCC010000766          243 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.772, whole genome shotgun sequence.
ACCESSION   DAZUCC010000766 DAZUCC010000000
VERSION     DAZUCC010000766.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.772"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>243
                     /locus_tag="ACHY09_005641"
     CDS             <1..>243
                     /locus_tag="ACHY09_005641"
                     /inference="COORDINATES: protein motif:HMM:NF015790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFS3888408.1"
                     /translation="YMPDMRLFKSKNTKLELRIRKPLFARGFRYRLHNSGLPGRPDMY
                     FPKYNAVIFIHGCFWHGHDNGCPISHTPKNNESYWQ"
BASE COUNT           74 a           44 c           48 g           77 t
ORIGIN      
        1 ttatatgcct gatatgaggt tatttaaatc aaaaaacacc aaacttgagt taagaatcag
       61 gaagccgttg tttgccagag ggtttcgtta tcgattgcat aatagtggtt tgcctggtag
      121 accagatatg tatttcccta aatacaatgc agtcattttt atccatggct gtttttggca
      181 tgggcatgat aacggctgcc ctatatctca cactcctaaa aataatgaat cgtactggca
      241 aaa
//
LOCUS       DAZUCC010000767          242 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.denovo.773, whole genome shotgun sequence.
ACCESSION   DAZUCC010000767 DAZUCC010000000
VERSION     DAZUCC010000767.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.denovo.773"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>242)
                     /locus_tag="ACHY09_005642"
     CDS             complement(<1..>242)
                     /locus_tag="ACHY09_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001541714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HFS3888409.1"
                     /translation="YDLEQATEKSFETKSIKPIGAEAIRQIGGWGGAVAGAKIGTVIG
                     ASLGVTTGPGAVATGIIGGIVFGAAGYFGADWVADY"
BASE COUNT           61 a           77 c           34 g           70 t
ORIGIN      
        1 ataatccgct acccaatctg caccaaaata accagctgca ccaaaaacaa tcccgccaat
       61 aatcccggta gccactgctc ctggcccagt agtaacaccc agggacgcgc ctatcactgt
      121 tccaatttta gcaccagcta ctgctcctcc ccatcctcca atttgcctta ttgcttctgc
      181 acctataggt tttatgcttt ttgtttcaaa cgatttttca gttgcctgtt ctaaatcata
      241 tg
//
LOCUS       DAZUCC010000768        15184 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.guided.122, whole genome shotgun sequence.
ACCESSION   DAZUCC010000768 DAZUCC010000000
VERSION     DAZUCC010000768.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.guided.122"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(262..1239)
                     /gene="epmA"
                     /locus_tag="ACHY09_005643"
     CDS             complement(262..1239)
                     /gene="epmA"
                     /locus_tag="ACHY09_005643"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFS3888410.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1564..3372
                     /gene="frdA"
                     /locus_tag="ACHY09_005644"
     CDS             1564..3372
                     /gene="frdA"
                     /locus_tag="ACHY09_005644"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFS3888411.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAVIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3365..4099
                     /gene="frdB"
                     /locus_tag="ACHY09_005645"
     CDS             3365..4099
                     /gene="frdB"
                     /locus_tag="ACHY09_005645"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFS3888412.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4110..4505
                     /gene="frdC"
                     /locus_tag="ACHY09_005646"
     CDS             4110..4505
                     /gene="frdC"
                     /locus_tag="ACHY09_005646"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFS3888413.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4516..4875
                     /gene="frdD"
                     /locus_tag="ACHY09_005647"
     CDS             4516..4875
                     /gene="frdD"
                     /locus_tag="ACHY09_005647"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFS3888414.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            4938..6071
                     /locus_tag="ACHY09_005648"
     CDS             4938..6071
                     /locus_tag="ACHY09_005648"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFS3888415.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6160..6693
                     /gene="blc"
                     /locus_tag="ACHY09_005649"
     CDS             6160..6693
                     /gene="blc"
                     /locus_tag="ACHY09_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFS3888416.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6694..7011)
                     /gene="sugE"
                     /locus_tag="ACHY09_005650"
     CDS             complement(6694..7011)
                     /gene="sugE"
                     /locus_tag="ACHY09_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFS3888417.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7186..7332)
                     /gene="ecnB"
                     /locus_tag="ACHY09_005651"
     CDS             complement(7186..7332)
                     /gene="ecnB"
                     /locus_tag="ACHY09_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFS3888418.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7443..7568)
                     /gene="ecnA"
                     /locus_tag="ACHY09_005652"
     CDS             complement(7443..7568)
                     /gene="ecnA"
                     /locus_tag="ACHY09_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFS3888419.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7620..8186)
                     /gene="efp"
                     /locus_tag="ACHY09_005653"
     CDS             complement(7620..8186)
                     /gene="efp"
                     /locus_tag="ACHY09_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS3888420.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8228..9256
                     /gene="epmB"
                     /locus_tag="ACHY09_005654"
     CDS             8228..9256
                     /gene="epmB"
                     /locus_tag="ACHY09_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFS3888421.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9491..10360
                     /locus_tag="ACHY09_005655"
     CDS             9491..10360
                     /locus_tag="ACHY09_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFS3888422.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMVENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10410..10763)
                     /locus_tag="ACHY09_005656"
     CDS             complement(10410..10763)
                     /locus_tag="ACHY09_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFS3888423.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(10901..12547)
                     /gene="groL"
                     /locus_tag="ACHY09_005657"
     CDS             complement(10901..12547)
                     /gene="groL"
                     /locus_tag="ACHY09_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS3888424.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(12591..12884)
                     /locus_tag="ACHY09_005658"
     CDS             complement(12591..12884)
                     /locus_tag="ACHY09_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS3888425.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13160..14416
                     /gene="yjeH"
                     /locus_tag="ACHY09_005659"
     CDS             13160..14416
                     /gene="yjeH"
                     /locus_tag="ACHY09_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFS3888426.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLSRLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14432..14908)
                     /locus_tag="ACHY09_005660"
     CDS             complement(14432..14908)
                     /locus_tag="ACHY09_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFS3888427.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
BASE COUNT         3740 a         3919 c         3792 g         3733 t
ORIGIN      
        1 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaaaca tccttcattt
       61 cagcgcgtgt ataaaacgcg cccactgaac tgcactgccc aacattgagc gggcagcgta
      121 aaaaccaata cagaattgat acctggatga tatcaaatat ccagtcttaa attgtggcct
      181 ttagcggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgcggt
      241 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      301 tgtctccgcg cccagcgcca acatcaccag acgatcaacg cctaatgcca cgccggaaca
      361 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      421 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      481 cgtcaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      541 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      601 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      661 ttgtagaagc gtgtcgcggt cttcttcagt atcggcaacg ttgctcaaat ccagtttcgc
      721 tgccacttcc cgcagttgcg ttttgtcggc agagagtggg tcaatttcca gataacgcaa
      781 gaaagcttgt tgataagaaa ggctttctgc tgccggacag tccagcacct gttgcaagag
      841 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
      901 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
      961 gaataccggc ccacaaccag caaccagcag gcgtttcata tggtattccg ggctggtcat
     1021 taaccagaga ttcatcccct gcgaatgccc ggggcctacg aaacgtgtct caaacgggac
     1081 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1141 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg
     1201 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1261 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1321 aagggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1381 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1441 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1501 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1561 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1621 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1681 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1741 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1801 caggatgtcg tggattattt cgtccaccac tgccccaccg aaatgaccca actggaactg
     1861 tggggatgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     1921 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     1981 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtactg
     2041 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2101 acgctggtgc agatccgtgc taacgcggtc gttatggcta ccggcggtgc gggtcgcgtt
     2161 tatcgttaca ataccaatgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2221 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2281 tccggtatcc tgatgaccga aggctgccgc ggtgaaggcg gtattctggt caacaaaaat
     2341 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2401 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2461 cgtaaaggca acactatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2521 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2581 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2641 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2701 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gttctaactc cctggcggaa
     2761 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2821 aatggcaacg aagcggtaat tgaagcgcag gcagctggcg ttgaacaacg tttgaaagat
     2881 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggct
     2941 atggaagaag gctgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3001 ctggcagagc tgcaggaacg cttcaaacgc gtgcgcatca ccgacacttc cagcgtgttc
     3061 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3121 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3181 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3241 ggcacgactc gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3301 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3361 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3421 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3481 tgcgctgggc tacatcaaag acaacctggc accggacctg agctaccgct ggtcctgccg
     3541 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3601 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3661 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3721 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3781 gatggccaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3841 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     3901 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     3961 gaacggcgta tggagctgta ctttcgtggg ctactgctcc gaagtctgcc cgaaacacgt
     4021 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4081 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4141 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttctacatg ctgcgcgaag
     4201 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4261 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4321 tcattaacct gatcactctg gcggcagccc tgctgcacac caaaacctgg tttgagctgg
     4381 caccaaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4441 aaagcctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4501 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4561 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4621 gttggtattc tgctgccact ggggctgttt ccgggtgacg cactgagcta cgagcgcgtt
     4681 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4741 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat ccacgtacct
     4801 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4861 gtcgttacaa tctaacgcat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     4921 tatggaaacc agaccctatg ttcaaaatga cgctctgcgc cttattaatt accgcctctt
     4981 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5041 ttatagagca acaaaagatc cccggtatag cggtggcggt aatttatcag ggtaaacctt
     5101 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5161 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5221 ctcgagggga aatcaagtta agcgatcccg cgacaaaata ctggcctgaa cttaccgcta
     5281 aacaatggaa tgggatcaca ctattacatc tcgcgaccta caccgctggc ggcctgccat
     5341 tgcaggtgcc ggatgacgtt aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5401 aacctgcatg ggctccagga acacaacgtc tgtatgccaa cgccagtatt gggttgttcg
     5461 gtgcactggc ggtgaaacca tccggtttga gctttgaaca ggcgatgcaa actcgtgttt
     5521 tccagccact caaactcact catacgtgga ttaatgtgcc gtccgcagaa gaaaagaatt
     5581 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5641 aggcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5701 aaccccttga tatcactgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5761 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5821 atcctgacat catcattaac ggcagtgaca ataaaattgc actggcagca cgccccgtaa
     5881 aagcgattac gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     5941 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6001 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6061 ctctacagta aaatttcatc gggtccgaat ttttggacct tttctccgct tttccttgct
     6121 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6181 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccccgtggc
     6241 gtgaccgtag tgaataattt cgatgctaaa cgctatctcg gtacctggta tgagattgcc
     6301 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt
     6361 gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagaga aatgtggcag
     6421 cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca
     6481 ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat
     6541 gcgctggttt gcggtccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6601 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6661 aagtttattt gggtacagca gcccggtagt tagttagtga gtgctgagtt tcagaccaat
     6721 aatccccaat acgattaacg ccagactcgc caggcgcatc gggttagcgg actcgccgag
     6781 cagcacaatg ccggtgatgg ccgcgccgac tgcgccaata cccgtccaca cggcataagc
     6841 cgtccctact ggtaacgatt tcatcgccca ggcaagtagc gccatactga caatcatcgc
     6901 cgtcacagta ataacacttg gcgtcaaacg actaaagccg tgggtatatt tcaggccaac
     6961 ggcccatacc acttccagca ggccagcaat aactaagata atccaggaca tatcaggctc
     7021 cggagaattg gggccgtccc cggtgaaaag aggcgtttgc aggtcgtcct gcaaagctga
     7081 tggttgaacg gatattttgc caggtggcag gggaatttca actattacct ttgtgactat
     7141 cgcaaaaaaa caaacggcac gacacagctg tcgtaccgtt attgcttact gctgcgcttt
     7201 cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt
     7261 ggtgttgcaa gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt
     7321 cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt
     7381 aataatagac aacatcgcca aaaatgatgg aaatctgatg atttttcgga ggagaagaaa
     7441 aattagctgg cagcgcggga gatggagttg ccgagatgtt taatgtcttc accaaaaccg
     7501 cgagcggtgt tacagcccgt gagcagcgca ctggcaagca aaacaagaac gataaggcgt
     7561 ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat
     7621 tacttcacgc gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatc
     7681 tgtacgaaca gcggaacttt aaccacagcg ccagtagaca gggtagctgg tttgccgcca
     7741 gtacctgcgg tatcaccttt caggcccgga tcggtatcaa cgatttccag ctcaacgaag
     7801 ttcggcggag taacggagat cggctgacca ttccacagag ttacgataca ctctgcctga
     7861 tccagcaacc atttggcgtt gtcgccaatt gcttttgcat cagcagacag ctgctcgaaa
     7921 gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt caggttcata
     7981 tcgacaacat cagcaccttc agcggaatca gtagatttga aggttttttc tacgcgagta
     8041 ccggtcagca gacgacgcag tttaacgcga gcaaatgcct ggcctttacc cggttttacg
     8101 aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag accagcacga
     8161 aaatcgttgc tatagtacgt tgccataagg ccctctgaaa tttgttaatt ggtagctaag
     8221 ccacaaaatg gcgcatattg taaccctaaa taccccatcc agagaagatt ggttaacgca
     8281 acttgccgat gttgtgaccg atcctgatga acttctgcgt cttttgaata tagacgcgga
     8341 cgaaaaactg ttggccgggc gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc
     8401 atttatcgat cgcatggaga aaggcaatcc gaacgatccg cttttgcgtc aggtacttac
     8461 ctcgcaagat gagtttgttg tcgcgcccgg attctccacc gacccgctgg aagaacagca
     8521 cagcgtagtg ccaggtttgt tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg
     8581 cggctgcgcg gtaaattgcc gctattgctt ccgtcgccac ttcccctatg ccgaaaatca
     8641 gggcaacaag cgtaactggc agactgcact tgagtatgtt gctgcgcatc cggaactgga
     8701 cgagatgatt ttctccggcg gcgatccgct gatggcgaaa gatcacgagc tggactggtt
     8761 gctcacacaa ctggaagcca tcccgcatat caaacgtctg cggattcaca gccgtctgcc
     8821 gattgtgatc ccggcacgca tcacagatgc gctggttgaa cgcttttctc attctacgct
     8881 gcaaatcttg ctggtgaatc acattaacca tgccaatgag gtagatgaaa cattccgtca
     8941 ggcgatggct aagttgcgcc gggtaggcgt tactttgctc aaccagagcg ttctgttacg
     9001 tggtgtgaac gataacgcgc aaacgctggc aaacctgagt aatgcgttgt tcgatgcggg
     9061 cgtgatgccg tattacctgc atgtgctcga taaagtacag ggcgcggcgc attttatggt
     9121 gagtgatgac gaagcacggc agattatgcg tgagttgctg acactggtgt cgggatatct
     9181 ggtgcctaaa ctggcgcgag aaattggcgg cgaacccagc aaaacgccgc tggatctcca
     9241 gctacgccag cagtaactga ttaaacaggg aagtttaatc tttgcacgtt gcataacctt
     9301 taattattca tatacattgc tgttattact ttttatataa ccaacaccaa taattaacct
     9361 ttcttttctg tttatataga gtgagcacgg ctatttatgt tattttaaca actattacta
     9421 ccaagcccat ttatctggct tataaattaa aattaattat ttaattgaga aatggttagg
     9481 gagaacctac atggccataa gcatcaaggg tgttaatacc ggtgttattc gcaagagcaa
     9541 taatttcatt gcactggcgc tgaaaattaa agagcctcgc aataaagaat cgttgttctt
     9601 tctgtctgtc atggaactgc gcgaccttct gattgcgctg gaaagccgtc tgcatcaaaa
     9661 gcataaactg gatgctgctg cacgccttca gtatgagcaa gctcgcgata aagtcattaa
     9721 gaaaatggta gaaaatatcc cagaaattct ggttgatgag cttaaaaatg ccgatattaa
     9781 tcgtcgggtt aacacgctgg aacttaccga taatcagggc gaaaatctta ccttcgtatt
     9841 aacactacat gacggtagca aatgtgagtt agtcatcaat gaattacaga ttgaaatgct
     9901 ggcgcgagca atcattcatg caattaataa tgcggaaatg cgtgaactgg cattacgtat
     9961 tacttcactg ttagatttcc tgccgttata cgacgttgac tgccaggaca atggcaatct
    10021 ggaatatgac acctattccc aaccggaatg gaaacataac ttgttcaacc attatctggc
    10081 ggtgctttac cgctttaaag atgaaagtgg caaagaacaa tttagcggcg cagtggtgaa
    10141 aacgcgtgaa gcaacgccgg gtaaagaagt ggaggcgatc actcgccgaa tgcttgattt
    10201 cagcccccga ctgaaaaaac tcgcgggtgt gccatgtcag gtctatgttc gcaccgtagc
    10261 ggcaaataac gcccaaccgc taacccagga tcaatgctta cgcgcgctgc atcacttacg
    10321 tgttcaatcc accagcaaaa ctgcaccaca ggcgaaataa tctcatctgt tttactacga
    10381 ggctggaagt atccagcctc tggatcacat cagttcgggc acttataaac ctgaccgata
    10441 atctggctat ccgtcgggac aaaactggac aacattccct gcgacgggct gctgatgcca
    10501 taaatcacgt taccgcccat tgcagccgcc tggttgcgca gatcgtttgc tgcgccgcgc
    10561 atagaaccgc cctcttctcc gtgttgcccg gaaagccagt tgctttgctt acctgtcgca
    10621 gtaccaatca gctggcactc tgcgcctggc tgctcgtcca caatgcgtac actctgaccg
    10681 gcagcactca attcgttgct ggagctacaa cccgccatca gtagcgcggc accgacaatc
    10741 cctgctaagt attttacgtg catgttattc cccataatca atgagctgga cgccactcgc
    10801 gtcgtccgtg tctgaatctt atactagaaa gatgaccaaa agaaaaaccc ccagacattt
    10861 ctgcccgggg gtttgtttat ttctgcgagg tgcagggcaa ttacatcatg ccgcccatgc
    10921 cacccatgcc gcccataccg ccagcagcgc ctaagtcagc tgcatcgttt ttcggcaggt
    10981 cggtaaccat gcattcggtg gtgatcatca ggccagccac agaagctgcg tactgcagag
    11041 cagaacgagt tactttggtt ggatccagga tacccatgtc gatcatgttg ccgtattctt
    11101 cggttgctgc gttgtaaccg tagttgccgt cgccgccttt aacggtgtta gcaacaacag
    11161 acggttcttc gccgcagttc aatacgatct gacgcagcgg agcttccatt gcacgcagtg
    11221 caactttgat acccacgttc tggtcttcgt tctgaccacg caggtcagcc agtttagacg
    11281 ctacgcggat cagcgcaaca ccaccaccag caaccacgcc ttcttctacc gcagcacggg
    11341 tcgcgtgcag ggcatcttca acgcgtgctt ttttctcttt catttcaact tcggtagcag
    11401 cacccacttt gataactgca acgccgcctg ccagtttcgc tacgcgctcc tgcagttttt
    11461 cacggtcgta gtcagaagtt gcttcttcaa tctgctgacg gatctgagca acacggccct
    11521 ggattgcagc ttcttcaccc acgccatcaa tgatggtggt ggtgtctttg ttgatcacaa
    11581 cacgtttagc ctgacccagg tcttccaggg ttgctttttc cagctccata ccgatctctt
    11641 cagagattac ggtaccgcca gtcagggttg cgatatcctg cagcatagct ttacgacgat
    11701 cgccgaagcc cggagcttta accgcagcaa ctttcacgat gccacgcatg gtgttaacaa
    11761 ccagagttgc cagcgcttcg ccttctacat cttcagcgat gatcagcagc ggtttgcctg
    11821 ctttggcaac agcttccaga accggcagca tttcgcggat gttggagatt ttcttgtcag
    11881 ccagcaggat gaacgggctt tccagttcta ctgcgccagt ttccggcttg ttgataaagt
    11941 aaggagacag gtagccacgg tcgaactgca taccttcaac cacgtccagt tcgtcctgca
    12001 gaccggtacc gtcttcaacg gtgataacgc cttctttacc gactttgtcc atcgcttcag
    12061 cgatcagttt acctacggtt tcgtcggagt tagcggagat ggtaccaacc tgagcaatcg
    12121 ctttagagtc agagcatggt acggacagcg ctttcagttc ttcaactgca gcggtaaccg
    12181 ctttgtcgat accacgtttc aggtccatcg ggttcatgcc cgcagcaaca gctttcagac
    12241 cttcagtgat gatagcctga gccagtacgg ttgcagtggt ggtaccgtcg cctgcagcgt
    12301 cgttcgcttt ggaggcaact tctttcacca tctgcgcacc catgttttcg aacttgtctt
    12361 ccagttcgat ttcacgagca acggaaacac catctttggt gatggtcggt gcaccgaaag
    12421 atttatccag aactacgtta cggcctttcg gaccgagggt aactttcact gcatctgcca
    12481 gtacgtttac gccgcgcagc attttcacac gagcgtcgtt accgaatttt acgtctttag
    12541 ctgccattat ctttattcct taaattcgta tgttcagtgt cgtgcgcgga ttacgcttca
    12601 acaattgcca gaatgtcgct ttcggacatg atcaacactt cttcattgtc gatcttctcg
    12661 gatttcacac cgtagccatc gttgaaaata acgatgtcgc caactttcac gtccagcggc
    12721 ttcacttcgc cattttcaag gatacggcca ttgccgacag ccagcacttc gccgcgggtg
    12781 gatttagccg ctgcagagcc ggtcagaacg atgccgccag cagatttagt ttcaacttct
    12841 ttacgcttga cgatcacgcg atcatgcaat ggacgaatat tcattgataa ctctcctttg
    12901 agaaagtccg tatctgttat gggtgacgcc ggagcttacg tggtttcccg gctggtgacc
    12961 agagaaatgg ggatgaggct tcgccccttc aagggggaaa aagaaaaaaa attctgatca
    13021 gcacaaaatc gggtgaaaac cctgattcac ctcacatttc atcgcaattt cttcatcgcc
    13081 gtcataagcg aatctgattg tgctaccatc gaaaatctac gcagttgccc aaaatttggg
    13141 cgcaatcgga catcaaccca tgagtggact caaacaagaa ctggggctgg cccagggcat
    13201 tggcctgcta tcgacgtcat tattaggcac tggcgtgttt gccgttcctg cgttagctgc
    13261 actggtagcg ggcaataaca gcctgtgggc gtggcccgtt ttgattatct tagtgttccc
    13321 gattgcgatt gtgtttgcga ttctgggtcg ccactatccc agcgcaggcg gcgtcgcgca
    13381 cttcgtcggt atggcgtttg gttcgcggct tgagcgagtc accggctggc tgtttttatc
    13441 ggtcattccc gtgggtttgc ctgccgcact acaaattgcc gccgggttcg gccaggcaat
    13501 gtttggctgg catagctggc aactgttgtt ggcagaactc ggtacgctgg cgttggtgtg
    13561 gtatatcggt actcgcggtg ccagttccag tgcaaatcta caaaccgtta ttgccggact
    13621 tatcgtcgcg ctgattgtcg ctatctggtg ggcgggcgat atcaaacctg cgaatatccc
    13681 cttcccggca cctggtaata tcgaacttac cgggttattt gctgcgttat cagtgatgtt
    13741 ctggtgtttt gtcggtctgg aggcatttgc ccatctcgcc tcggaattta aaaatccaga
    13801 gcgtgatttt cctcgtgctt tgatgattgg tctgctgctg gcaggattag tctactgggg
    13861 atgtacggta gtcgtcttac acttcgacgc ctatggtgaa aaaatggcgg cggcagcatc
    13921 gcttccaaaa attgtagtgc agttgttcgg tgtaggagcg ttatggattg cctgcgtgat
    13981 tggctatctg gcctgctttg ccagtctcaa catttatata cagagcttcg cccgcctggt
    14041 ctggtcgcag gcgcaacata atcctgacca ctacctgtca cgcctctctt ctcgccatat
    14101 cccgaataat gccctcaatg cggtgctcgg ctgctgcgtg gtgagcactt tggtgattca
    14161 tgctttagag atcaatctgg acgctcttat tatttatgcc aatggcatct ttattatgat
    14221 ttatctgtta tgcatgctgg caggctgtaa attattgcaa ggacgttatc gactactggc
    14281 ggtggttggc gggctgttat gcgttctgtt actgacaatg gtcggctgga aaagtctcta
    14341 tgcgctgatc atgctggcgg ggttatggct gtttctgcca aaacgaaaaa cgccggaaaa
    14401 tggcataacc acataatccg gcgtttcgac attaatcctg gcgatcgtct ttatgatcaa
    14461 ggcggtcgcg gtcatcatcc tttcgctggt actcaccatc aaaagtatta ccgccaccgg
    14521 tcccggcgct aaaaccgccg cctggcatgc gagaaaagcg caaatgcggc atcaacttca
    14581 ctgtcagatg cttttgcacc ggcggcaaca aaagcagaag accgaggaag tcggtgaaaa
    14641 agcccggtaa taaaagcagc aaaccggcaa tgatcagcga aacactttta atcatctccg
    14701 ccgctgggtt ttcacccgcc gccatttttt gctgcatcag cacgaaattc ttaaagccct
    14761 ggttacgtac cagtgacata ccgataactg acgtgaatat aaccagcacg agagtcagca
    14821 atacccccaa tacatgggca acctgaataa agattgaaat ctcaatatag acataaagga
    14881 aaatggcaat aaaaggtaac cagcgcaaag gtttctcctg taatagcagc cggttaaacc
    14941 cggctacctg aatgggttgc gaatcgcgtt cagcttatat tgtggtcatt agcaaaattt
    15001 caagatgttt gcgcaactat ttttggtagt aatcccaaag cggtgatcta tttcacaaat
    15061 taataattaa ggggtaaaaa ccgacactta aagtgatcca gattacggta gaaatcctca
    15121 agcagcatat gatctcgggt attcggtcga tgcaggggat aatcgtcggt cgaaaaacat
    15181 tcga
//
LOCUS       DAZUCC010000769         5513 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.guided.267, whole genome shotgun sequence.
ACCESSION   DAZUCC010000769 DAZUCC010000000
VERSION     DAZUCC010000769.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.guided.267"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..326
                     /locus_tag="ACHY09_005661"
                     /pseudo
     CDS             120..326
                     /locus_tag="ACHY09_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            322..674
                     /gene="prfH"
                     /locus_tag="ACHY09_005662"
                     /pseudo
     CDS             322..674
                     /gene="prfH"
                     /locus_tag="ACHY09_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHY09_005663"
     CDS             complement(731..2188)
                     /gene="pepD"
                     /locus_tag="ACHY09_005663"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFS3888428.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2449..2907
                     /gene="gpt"
                     /locus_tag="ACHY09_005664"
     CDS             2449..2907
                     /gene="gpt"
                     /locus_tag="ACHY09_005664"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS3888429.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            2999..4243
                     /gene="frsA"
                     /locus_tag="ACHY09_005665"
     CDS             2999..4243
                     /gene="frsA"
                     /locus_tag="ACHY09_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFS3888430.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRYWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4301..4702
                     /gene="crl"
                     /locus_tag="ACHY09_005666"
     CDS             4301..4702
                     /gene="crl"
                     /locus_tag="ACHY09_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFS3888431.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(4741..>5513)
                     /locus_tag="ACHY09_005667"
     CDS             complement(4741..>5513)
                     /locus_tag="ACHY09_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFS3888432.1"
                     /translation="RLAFAGLKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQ
                     TDNFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDF
                     GGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMT
                     PITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYY
                     FNKNMSAFVDYKINQLESDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1346 a         1354 c         1423 g         1390 t
ORIGIN      
        1 tgcaggaagg cgacaagcga gtgaatcccc aggagcttac taaagtaagt gactggggtg
       61 aacgaacgcg gtcgcagtac atgcaacttg aagtatgacg agtataaatg gaatgataca
      121 tgggcaaata tattcgtccc ttatccgatg cggtatttac catcgcatct gatgacctgt
      181 ggatcgagag tttagcgatc caacaattac acaccacggc aaatttaccc aacatgcagc
      241 gcgttgtggg gatgccagat ttacatcccg gacgcggcta cccgattggc gcagcgttct
      301 tctctgttgg tcgtttttac ccggcaagac gtcgcggtaa cggtgctgga aacagaaacg
      361 ggccgctact ctgacacgct gcgttcggcg ctgatttctc tggatggcga taatgcatgg
      421 gcattaagcg aaagttggtg cggcactatt cagtgtattt gtccgagtcc gtatcggcct
      481 catcatgggc gcaaaaactg gtttctgggc atgctaacaa gcggctggcg cgattgctga
      541 ttgcctggaa gctggaacaa cagcaacagg aaaatagcgc ggcgctgaaa tcgcagcggc
      601 gaatgttcca tcaccagatt gaacgtggca acccgcgacg aacgttcaca gggatggctt
      661 ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca tccggcagca
      721 aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagtagt gtccagtaat
      781 gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg atggttggcc
      841 cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat tccaggcccg
      901 cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt tcacgtacca
      961 gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct ttcgcttcgg
     1021 ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag tctttaccgc
     1081 tgtcgatcag agaacggatc aggcagtgaa tttctacgtt attgtcagtc atggtcacca
     1141 caccgacgtt cagggaggtt tcgaccacac cttttgccac gtcggagttg cggatcacac
     1201 cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca atcagggcag
     1261 ctttatcgtt cgcaacagag tccagcaaca aggccagatt tttctctttc tctgccagct
     1321 cgtttttcag gatctcctga taggtattca ccagagattt caaagcgtcg actttatcag
     1381 ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt gtgccgccgt
     1441 tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc cgccaggaag cgcaccagca
     1501 gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg cctttcaggc
     1561 cttttaaggt taacttgaag gtttcaaagc cagctggaac cgcttcacga tctaaatgca
     1621 ggttggaggt gaagtcgata cctcccgcac aacccatgta gatttcacct tcttcttcgg
     1681 agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagcca aaagcaccgt
     1741 ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt
     1801 cgtcagccag aaccgccagt gcagaggcca taccaatgcc gttatccgca cccagcgtgg
     1861 tgccgcgtgc tttaacccat tcgccatcaa tataaggctg gataggatct ttggtgaagt
     1921 catgcacggt gtcgttattt ttttgcggca ccatatcgag gtgggcctgc aagacgaccg
     1981 gtttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta cctacctgat
     2041 cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct
     2101 cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct
     2161 gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca
     2221 gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata
     2281 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
     2341 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
     2401 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
     2461 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
     2521 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt accgggtgcg
     2581 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
     2641 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
     2701 atcgttattg atgacctggt agataccggt ggtactgcgg ttgcgattcg tgaaatgtat
     2761 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgat
     2821 tatgttgttg atatcccgca aaatacctgg atcgaacagc cgtgggatat gggcgtcgta
     2881 ttcgtgccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     2941 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     3001 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     3061 gacgctagtc cgccgcttta gtcacggcgc acaactgcct gtgcagtcgg cccttgatgg
     3121 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     3181 tgacccccgt gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg ccgaacgtac
     3241 cgacgatgat ttatacgata cggtgattgg ctaccgcggc ggcaactgga tttatgagtg
     3301 ggctacccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag
     3361 cggtcgatac tggctgcatg cggctacgtt gtacaacatt gctgcctatc ctcatctgaa
     3421 aggagatgac ctggccgagc aagcgcaggc tttgtcaaac cgcgcctatg aagaggccgc
     3481 tcagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg gtgcgcccat
     3541 caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg
     3601 tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt ttgcgccgcg
     3661 cggcattgct atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct
     3721 cacccaggac tccagcctgt tgcatcagca cgttttaaag gcgctgccta acgtaccgtg
     3781 ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg
     3841 tctggcatac cttgaatcgc cgcgtctgaa agtggttgcc tgtcttggtc cggtagttca
     3901 taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc ttgacgttct
     3961 ggccagtcgt ttagggatgc atgatgcttc cgatgaagcg ttgcgagtgg agttgaatcg
     4021 ctattcatta aaagtgcaag gattgctggg acgtcgctgc ccaacgccaa tgttatcagg
     4081 ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca cgtcgtcatc
     4141 tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt ttgacaaagg
     4201 ccttcaggaa atcacagact ggatcgaaaa acgtttgtgt taaaaatttg ctaaatttta
     4261 ccaatttggt aaaacagttg catcacaaca ggagattgca atgacgttac cgagtggaca
     4321 cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa
     4381 gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg tgaaaccggc
     4441 accggaagtg cgtgagttct ggggctggtg gatggaactg gaagcgcagg aatcccgttt
     4501 tacctacagt taccagtttg gtctgttcga taaagcaggc gactggacga gtgttcagat
     4561 aaaagatgcc gaagtggttg aacgactgga gcacaccctg cgtgaatttc acgagaagct
     4621 gcgtgagctg ctggcgacgc tgaatctgaa gctggaaccg gcagatgatt ttcgtgatga
     4681 accggtgaag ttaacggcgt gaatgaaatg tgccggatgc atcacatccg gcaatattca
     4741 ttaaaactga tacgtcatcc caaccgcgac aatatcatca ttattaatat ttaatttgtt
     4801 atcgctttcc agttggttga ttttataatc aacaaacgct gacatatttt tgttgaaata
     4861 atacgtagca ccaacatcga cataattgac cagatcttca tcaccgatgc cctcaatatc
     4921 tttccctttc gataagacat aacccagcga tggacgcaga ccaaagtcaa actggtattg
     4981 agcgaccgct tcaaagttct gcgtcttatt ggcaaagccg ccagttattg gcgtcatttt
     5041 gcgtgtttca gaatagaaag ttgccagata aatattattg gcatcgtatt tcagacctgt
     5101 tgcccatgct tctgcacgct tgcctgtgcc acggctttgc aggttctgct cgttggtgcg
     5161 atcagagttg gtataggctc cactgatggc gaaatcgctg ccaccaaagt catatgtcaa
     5221 tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg cggttttcgt ttttcccttg
     5281 atattgcagg gttaagttca ggccatcgat aacgccgaag aagtcggtgt tccgatacgt
     5341 cgccagaccg ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg agtcgccacc
     5401 aaattccggg aacatatcgg tccaggcttc cacgtcatac aacgcgccca ggttacgacc
     5461 ataatcgaaa gaacccaaat ctttatattt caacccggca aaagcgagac gtg
//
LOCUS       DAZUCC010000770         1499 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802H72877
            SAMN43251503-rid23076113.guided.449, whole genome shotgun sequence.
ACCESSION   DAZUCC010000770 DAZUCC010000000
VERSION     DAZUCC010000770.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251503
            Sequence Read Archive: SRR30292948
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349799.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349799.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:00:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,669
            CDSs (total)                      :: 5,586
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 83
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 80 of 178
            Pseudo Genes (incomplete)         :: 123 of 178
            Pseudo Genes (internal stop)      :: 45 of 178
            Pseudo Genes (multiple problems)  :: 56 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251503-rid23076113.guided.449"
                     /serotype="O146:H28"
                     /isolate="1802H72877"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHY09_005668"
     CDS             132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHY09_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFS3888433.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHY09_005669"
     CDS             1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHY09_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFS3888434.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          412 a          304 c          358 g          425 t
ORIGIN      
        1 aagcaatcgg tcactggttc gaatccagta caacgcgcca tacttatttt ttctggctcg
       61 cttttgcggg ccttttttat atctgcgccg ggtctggtgc tgattacttc agccaaaagg
      121 aatacctgta tatgaagtgt atattgttaa aatgggtact gtgcctgtta ctgggctttt
      181 cttcggtatc ctattcccgg gaatttacga tagacttttc gacccaacaa agttatgtct
      241 cttcgttaaa tagtatacgg acagaaatat cgaccccact tgaacatata tctcagggga
      301 ccacatcggt gtctgttatt aaccacaccc caccgggcag ttattttgct gtggatatac
      361 gagggcttga tgtctatcag gcgcgttttg accatcttcg tctgattatt gagcaaaata
      421 atttatatgt ggccggattc gttaatacgg caacaaatac tttctacagg ttttcagatt
      481 ttgcacatat atcagtgccc ggtgtgacaa ctgtttccat gacaacggac agcagttata
      541 ccactctgca gcgtgtcgca gcgctggaac gttccggaat gcaaatcagt cgtcactcac
      601 tggtttcatc atatctggcg ttaatggagt ttagtggaaa tgccatgacc agagatgcat
      661 ccagagcagt tctgcgtttt gtcactgtca cagcagaagc cttacggttc aggcaaatac
      721 agagagaatt tcgtctggca ctgtctgaaa ctgctcctgt ttatacgatg acaccggaag
      781 aagtggacct cacactgaac tgggggagaa tcagcaatgt gcttccggag tttcggggag
      841 agggtggtgt cagagtgggg cgaatatcct ttaataatat atcagcgata ctgggcacag
      901 tggcggttat actgaattgc catcatcagg gggcgcgttc cgttcgcgcc gtgaatgaag
      961 agatacaacc agaatgtcag ataactggcg acaggccagt tataaggata aacaatacgt
     1021 tatgggaaag taataccgca gctgcttttc tgaatcgcag ggctcactct ttaaatacat
     1081 ccggagaata acaggagtta aatatgaaga agatatttgt agcggcttta tttgcttttg
     1141 tttctgttaa tgcaatggca gctgattgtg caaaaggtaa aattgagttc tctaagtata
     1201 atgagaatga tacattcaca gtaaaagtgg ccgggaaaga gtactggact aaccgctgga
     1261 atctgcaacc gctactgcaa agcgcacagt taacaggaat gacggtaaca atcaaatcaa
     1321 atacctgtgc gtcaggttca ggatttgctg aagtgcagtt taattaatat cagaagcatt
     1381 gctggtttcg tggtgtgcag caatgtagtt acagtgtaat caatgtcaca attcagtcag
     1441 ttgaaggttg tctgcccgac tgagaatttg ttaaaaaaat cctgcatggt gaatccccc
//
